LOCUS       DASQKS010000001         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_766, whole genome shotgun sequence.
ACCESSION   DASQKS010000001 DASQKS010000000
VERSION     DASQKS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VE170_00005"
     CDS             complement(<1..365)
                     /locus_tag="VE170_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYJ13840.1"
                     /translation="MSSTDNVQSLPVLPLKNAVLFPFMLMPLTIGRANSIAATEAALA
                     SEGKDLVVVAQRDASMETPALTDLYSIGTRAVIKQMARKSDGSLEVVLLGVERVVLLK
                     LEQDGAHQTARIKPLPLPQ"
     gene            complement(362..742)
                     /locus_tag="VE170_00010"
     CDS             complement(362..742)
                     /locus_tag="VE170_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYJ13841.1"
                     /translation="MDGMTRDNLKRMAVLFSAAVHSSGEPYWQPAADIYKTRTGWLIK
                     YDLAGVRSEDIEVTILGSRITVRGMRRDWQLEDGASRYSMEISYNRFERTLELSCDVS
                     GAEVSFKGYEGILLLRLTCQGDMP"
     gene            complement(816..1244)
                     /locus_tag="VE170_00015"
     CDS             complement(816..1244)
                     /locus_tag="VE170_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ13842.1"
                     /translation="MSAPVTFRRILCAVDFSRDSLEAFHAAADMARLRSGFLHVLHVI
                     ETQPPGSGEAIIELTKKANAALAELIASAPSNGLALSSEVSSGDTAIEIVDRAKDWRA
                     DLIVLGARGVILIEDAIVGGTAEAVTRDAPCSVLVVRQRN"
     gene            complement(1264..1659)
                     /locus_tag="VE170_00020"
     CDS             complement(1264..1659)
                     /locus_tag="VE170_00020"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HYJ13843.1"
                     /translation="MQEEKTMTGEIARENWTRFFDDFSKQHQNWIVILEVLGRDIGEQ
                     LESTRLPLIGISADVKTAPPTIAIILGGRPDAHVTHIIHNPKRVFAKPPEEPAHEAIE
                     IEGDDGKITLVHFRHLHPEQAEFLLPPRL"
     gene            complement(1694..>2454)
                     /gene="pgk"
                     /locus_tag="VE170_00025"
     CDS             complement(1694..>2454)
                     /gene="pgk"
                     /locus_tag="VE170_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYJ13844.1"
                     /translation="FFWHGPLGIWEIEPFAAGTRAVAQALAEETPERRHRSVVCGDSL
                     ARALRSFDLPFERIRHLSSGGASALAMLAGKPLPAVAALDNEVDLLAPVEKRPGKILL
                     AVDGSAPSIEAARKLSTLVAAEGAEIALVYVQKPAERDGKIPLMDSATQRRREFERRL
                     EAERVMAAGQAPLARQGLVVHHQIIASGDPADEILKVADELGVHLIAMGSHGRSGILG
                     IFLGSVSREVLDRARCPVLIVRPPGLAADLDGDG"
BASE COUNT          471 a          764 c          737 g          482 t
ORIGIN      
        1 tcctgcggca acggcagcgg ctttatgcgc gccgtctgat gagcgccgtc ttgctccaat
       61 ttcaggagca cgacgcgctc tacgccgagg agaaccacct ccagcgaccc gtccgacttg
      121 cgtgccatct gtttgatgac ggctcgcgtg ccgatggaat agagatcggt cagagccggg
      181 gtctccatgg aagcgtcgcg ttgagcgacc accacgagat cttttccttc gcttgccagg
      241 gccgcttcag tggccgcgat ggagttggcc cggccgatgg tcaaaggcat gagcatgaag
      301 ggaaacaaga cggcattctt gagcggcaag acgggcaaac tttgcacatt atcggtactg
      361 ctcacggcat atctccttgg caagtcaaac gaagcaacag aattccctcg tatcccttga
      421 acgaaacttc ggcgccggaa acatcgcagg acaactcgag agttcgctca aagcggttgt
      481 aagatatctc catcgaataa cggctggcgc cgtcttccaa ttgccagtcg cggcgcatgc
      541 cgcggacggt gatgcgcgag cccagaatcg tcacttcgat atcctctgat cggactcccg
      601 ccagatcgta cttgatcagc cagcccgtgc gcgtcttata gatgtcggcg gcgggttgcc
      661 agtagggctc accgctgcta tgaacggccg cgctaaaaag caccgccatc cgctttagat
      721 tgtctcgcgt cattccatcc atcggtgctc atccattcca gtccgcgggc gcctctcgac
      781 cgacgtttcc acatacgcgc caattcatac ccgcctcaat tcctctgcct tacgaccaag
      841 acggaacacg gcgcgtccct cgtcactgcc tcggcggtgc cgccgacgat agcatcctcg
      901 atcaagatca cgccgcgggc gcccaaaacg atcaagtcag cgcgccaatc cttggcccgg
      961 tcgacgatct caatagccgt atcgccgctg gagacttcgc tgctgagagc caaaccgttt
     1021 gacggcgccg aggcgatcag ctccgccagc gcagcgttgg ctttcttggt cagttcgatg
     1081 atcgcttcgc cggagccggg aggctgggtt tctatgacgt gcaagacgtg caagaagccg
     1141 gagcgcaggc gcgccatgtc ggcggcggcg tggaaagctt ccagcgagtc gcgcgagaag
     1201 tcgacggcgc agaggattcg tctgaacgtt acgggcgcac tcatggtaac tcgtcgcccc
     1261 ttctcagagc cgcggcggca gcaggaactc ggcctgttcg gggtgcaagt ggcgaaaatg
     1321 caccagggtg atcttgccgt cgtcgccttc gatctcaatc gcctcatggg ccggttcttc
     1381 cggtggcttg gcgaagacgc gcttgggatt gtggatgatg tgcgtgacgt gcgcatcggg
     1441 tcggccgccg aggatgatgg caatggtcgg cggcgcggtt ttcacatcgg cgctgatgcc
     1501 gatgagcggc agccgtgtcg attccagttg ctcgccaatg tctcggccga gcacttccag
     1561 gatcacgatc cagttttggt gttgcttgct gaaatcatca aaaaaacgag tccagttttc
     1621 gcgcgcaatc tcaccagtca tggttttttc ctcctgcata gggtttcatt tttcccagcg
     1681 atgtgagacc gccttaccca tcgccatcta ggtcagccgc caagccgggt gggcgcacga
     1741 tcaataccgg acaacgggcg cggtcgagca cctcgcgcga cacgctgccg agaaatatcc
     1801 cgagaatgcc gctgcggcca tgggatccca tggcgatcaa atgtacgccc aactcatcgg
     1861 ccaccttgag aatctcgtcg gcgggatcgc cgctggcgat gatctggtga tggaccacca
     1921 atccctgacg cgcaagcggc gcttggcccg cggccatcac gcgttcggct tcaagccggc
     1981 gttcgaactc gcgccggcgt tgtgtcgcgc tatccatcaa gggaattttt ccgtcccgct
     2041 ccgccggttt ttgcacgtaa acgagcgcga tctcggcgcc ctcggcggcg accagagtgc
     2101 tgagcttacg cgccgcttcg atcgagggcg ccgaaccatc gacggcgaga agaatcttgc
     2161 cgggccgttt ttcaaccggc gcgagcaaat cgacttcatt atcgagggcc gccaccgcgg
     2221 gtagcggttt tccggcgagc atggccaacg cacttgcgcc gccgctggag aggtggcgga
     2281 tgcgctcgaa gggcaggtca aaactgcgca aggcgcgggc taggctgtcg ccgcaaacca
     2341 cgctgcggtg gcggcgctcc ggcgtctctt cagccaacgc ctgggcgacg gcacgggtgc
     2401 cggcggcaaa cggttcgatt tcccagatgc ccaacggccc atgccaaaaa aaag
//
LOCUS       DASQKS010000002         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1681012, whole genome shotgun sequence.
ACCESSION   DASQKS010000002 DASQKS010000000
VERSION     DASQKS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681012"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VE170_00030"
     CDS             <1..1008
                     /locus_tag="VE170_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HYJ13845.1"
                     /translation="IEFAKTMGSVNNLKMLYLLTFADVKAVGPDVWNPWKGSLLGELY
                     VKTLNVLEDAEKGEFQREDLRAVLERIQDRVRRQLLEMPQPEAKVDDFIDAMPDRYFF
                     STSEAEIPKHFALMEKFRGKGALSYVEHFPERNCSSVVICTQDRPGLFASITGVLTSL
                     SLDILNARIFTASDGRILDVFRISHRGRPEVVMADAKWAKFKTVLDNVLEGKSDVVRL
                     VEAAKPSLYLLKRAPKVSTVINIDNHASDDFSIVEIFTDDRIGVLFTIAHNLHQLGLS
                     IHVAKISTNVDQVADVFYVTDSSGKQIAGSERVEEVRNYLFQSLAPENVAHERLAESV
                     H"
     gene            971..1891
                     /gene="xerD"
                     /locus_tag="VE170_00035"
     CDS             971..1891
                     /gene="xerD"
                     /locus_tag="VE170_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYJ13846.1"
                     /translation="MLLTKDSLSPYIDSFLSMLTVEKGLAKNTLDAYGRDLAGLSGFL
                     IEQKIADWREVDTHHIRAYLGVLRAKGLSPKSIARHLVTLRRLLRFLQSENLLKENPM
                     PTLLPTPGQRKLPQTLSAADIQSLLNQPDANEPLGARDQAMFELLYATGLRVSELVLL
                     QTQQVNFQGNYVIVKGKGSKVRAVPFGNYAREKLNAFMTDGRRRLLKGKSSPFVFINR
                     SGKPLSRQGFWKLIRRYALTAGIDKRVTPHTLRHSFATHLLEGGADLRSVQTMLGHAD
                     ISTTQIYTHVDGARLKKVHRQFHPRERNGS"
     gene            1991..2644
                     /locus_tag="VE170_00040"
     CDS             1991..2644
                     /locus_tag="VE170_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYJ13847.1"
                     /translation="MEDTIQKIAIWAIPVLVAVVFHEVSHGWVANQLGDSTAKNLGRL
                     TLNPIAHIDLFGTILVPAMLILVSSPFVFGYAKPVPVNFYNLRNPKRDMVWVALAGPV
                     TNLLLALAFVVIWRLFLPQSRADLGNSLVLTTLVLMVQAGLLINISLAVFNMLPLPPL
                     DGGRVLVGLLPEPLSSKVAGIERFGMLILIGLLYTRVIDRFIDAPTKFLLKLYLGIF"
     gene            2655..3632
                     /gene="trpS"
                     /locus_tag="VE170_00045"
     CDS             2655..3632
                     /gene="trpS"
                     /locus_tag="VE170_00045"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYJ13848.1"
                     /translation="MDTIVSGMRPTGRLHLGHYFGALTNWLKLQEQYRCYFFVADWHA
                     LTTDYATPQGINQNTIEMVMDWLSVGLDPAKAVVFRQSRVKEHAELFLLYSMIVPVPW
                     LERNPTYKDQIKELSGKDLSTYGFLGYPVLQAADITMYKANKVPVGVDQAPHVELTRE
                     IVRRFNQVYTPIFPEPEVLLTDTQKLPGLDGRKMSKSYGNAIMLSDSPKEIDQKLSKM
                     VTDPARVRRSDPGEPEKCPAFQFHKIYSTPEEIAEVSHGCRTAGIGCLECKKIMIKHV
                     VAQLAPITERRAALEQRPQDVEDILLAGNHVAEQKAGETMTEVREAMGL"
     gene            3629..>3949
                     /locus_tag="VE170_00050"
     CDS             3629..>3949
                     /locus_tag="VE170_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HYJ13849.1"
                     /translation="MSTRVELEIFEGPLDLLLHLIKKNEVSITDIPIATITEQYLATL
                     DLLQTLSLDVAGEFLVMAATLIHIKSRMLLPAGDDGGDDEDDGLDPRDELVRRLREYQ
                     RYKEA"
BASE COUNT          842 a         1058 c         1115 g          934 t
ORIGIN      
        1 attgagttcg cgaaaaccat gggtagcgtc aacaatttga aaatgctcta tctcctgacc
       61 ttcgccgatg tcaaagcggt ggggccggat gtttggaatc cttggaaggg ttcgcttctg
      121 ggcgagctct acgtcaagac attgaacgtc ctcgaagacg ccgaaaaggg cgagttccag
      181 cgcgaagatt tgcgcgcggt gctagaacgc atccaagacc gcgtccggcg ccaattgctc
      241 gagatgccgc aacccgaggc caaggtcgat gatttcatcg acgccatgcc cgatcggtat
      301 tttttttcca cttccgaggc cgaaattccc aagcacttcg ctttgatgga aaagtttcgc
      361 ggtaaaggcg cgctctcgta tgtcgagcat ttccccgaac gtaactgtag ttcggtggtg
      421 atctgtaccc aggatcggcc gggactgttc gcttccatca ccggcgtctt gacctcgctt
      481 agtctcgata ttctcaacgc gcggattttt accgccagcg acggacgcat cctcgatgtc
      541 tttcgcattt cgcatcgggg ccggccggaa gtggtcatgg ccgacgcgaa atgggccaag
      601 ttcaaaaccg tcttggacaa tgtgttggaa ggtaaaagcg acgttgttcg tttggtcgag
      661 gcggccaagc cgtcgctcta tctgctcaag cgcgcgccca aagtatccac ggtaatcaat
      721 atcgacaacc atgcatcgga cgacttttcc atcgtcgaga tttttaccga tgatcggatc
      781 ggcgtgctgt ttaccatcgc tcataacttg catcagttgg gactgtcgat ccatgtcgca
      841 aagatctcga ccaacgtcga tcaagtggcg gacgtgtttt atgtcaccga tagctccggt
      901 aaacaaatcg ccgggtccga aagagtcgag gaagtcagga actatctatt tcaaagtctg
      961 gcgccggaga atgttgctca cgaaagactc gctgagtccg tacattgact cattcctgtc
     1021 gatgctcacc gtcgagaagg gcttggcaaa aaataccctc gatgcctatg gccgcgatct
     1081 cgccggcctt tcaggctttc ttatcgagca aaagatcgcc gactggcgcg aagtcgacac
     1141 ccatcatatt cgcgcctacc ttggcgtgct gcgcgccaag ggactgagtc caaagagcat
     1201 cgcccgtcac ctggtgacgt tgcgccgact gttgcgcttt cttcaaagcg aaaacctgct
     1261 caaagaaaat cccatgccga cgctcttgcc gacgccggga cagagaaagt tgccgcagac
     1321 gctctcggcg gcggacattc aaagcctgct caaccagccg gacgccaatg aaccactggg
     1381 cgcccgcgac caggcgatgt tcgagctgct ctacgccacc ggcttgcggg tgtcggagtt
     1441 ggttcttttg cagacccagc aggttaattt ccaaggcaac tacgtcatcg tcaaaggcaa
     1501 gggctcgaaa gtgcgcgccg tgccgttcgg caattacgcg cgcgaaaagt tgaacgcctt
     1561 catgaccgat ggccggcggc gcttgcttaa ggggaaatcg agtccttttg ttttcatcaa
     1621 tcgttccggg aaaccgctga gccggcaagg tttctggaag ctgatccgcc gttacgctct
     1681 gaccgccggt atcgacaaac gcgtcacgcc gcacacgctg cgccactcgt tcgccaccca
     1741 tctcctcgaa ggcggcgccg acctgcgttc ggtgcaaacc atgctcggcc acgccgatat
     1801 ttcgacaaca cagatttata cccatgtgga cggcgcgcgc ttgaagaagg tgcatcgtca
     1861 gtttcatccg cgcgagcgga atggctcata gtttggatcg cgcatctgtt gatcgatcca
     1921 atcgctgctc cgccttgtgt tttcgcgctt cataccgtat ttctatgaga gtagtccccg
     1981 cgagaaagta ttggaagaca ccatccagaa aattgccatt tgggcgattc cggttcttgt
     2041 agctgtagtt tttcatgagg tttcccatgg ctgggtggca aaccagctgg gcgattcgac
     2101 ggctaaaaat ttgggccgac tgacgctcaa tccgatcgcg cacatcgatt tgtttgggac
     2161 gattctcgtt ccggcgatgc tgatcttggt cagttccccg tttgtcttcg gttacgccaa
     2221 gccggtgccg gtgaattttt acaacctgcg caatcccaag cgcgatatgg tgtgggttgc
     2281 cttggctggg ccggtgacga atttgctgct ggcgttggct ttcgtggtaa tctggcgact
     2341 gtttctgcct caaagcaggg cggacttggg aaactcactc gttttgacca ctctggtgct
     2401 gatggtccag gccggtttat tgatcaatat ctcgttggcg gtattcaata tgttaccgtt
     2461 gccgccgctg gacggtggcc gggtgctggt tgggcttttg ccggaaccgt tgtcatcgaa
     2521 agttgccggt atcgagcgct ttggaatgtt gattctgatc ggtcttctat atactcgcgt
     2581 gatcgatcga ttcatcgatg cgcccacgaa gtttttgtta aaattgtatt tgggaatttt
     2641 ttaattgagg agaaatggac accatcgtta gcggcatgcg gcccaccgga cgacttcatc
     2701 ttggccatta ttttggcgcg ctgaccaatt ggctcaagct tcaagagcag tatcgttgtt
     2761 actttttcgt tgccgattgg catgcgctga ccaccgatta tgcgacgccg caaggaatca
     2821 atcagaacac catcgagatg gtcatggact ggttgagcgt cggcctcgat ccggcgaagg
     2881 cggtggtgtt tcgccagtcg cgggtgaagg agcatgccga gttgtttctg ctctattcga
     2941 tgatcgtgcc ggtgccttgg ctcgagcgga atccgactta taaggatcag atcaaggaat
     3001 tgtccggtaa ggatctgtcg acctatggtt ttctcggtta tccggtgctt caggcggccg
     3061 atattacgat gtataaggcc aacaaagttc cagtaggcgt agatcaggcg ccccatgtgg
     3121 agctgactcg cgagatcgtg cgtcgtttca accaggttta cacgccgatc tttcctgagc
     3181 cggaggttct gctgaccgat acccagaagc ttcccgggct cgatgggcgt aagatgagca
     3241 agagctatgg caatgcaata atgctctcgg attcgcccaa ggagatcgat cagaagctga
     3301 gcaagatggt caccgatcct gcccgtgttc ggcgcagcga tcccggcgag ccggagaagt
     3361 gcccggcgtt tcaatttcat aaaatctatt cgacaccgga agagatcgcc gaggtttcgc
     3421 atggctgccg cacggcagga atcggctgtc tcgagtgtaa aaaaatcatg atcaaacatg
     3481 tcgtggcgca gttggcgccc atcacggaac ggcgcgcggc cttggagcag cggccgcagg
     3541 atgtcgaaga catcttgctc gccggcaacc atgtggcaga gcagaaagcc ggcgaaacca
     3601 tgactgaagt cagagaggcc atgggactgt gagcacccgc gtcgaattgg aaatattcga
     3661 gggtcctctg gacctgctgc ttcacttgat caagaagaac gaagtcagca ttaccgacat
     3721 tccgatcgcg acgatcaccg agcagtattt ggctaccctc gacctgttgc agactttgag
     3781 cctagatgtc gccggagaat ttttggtcat ggccgcgacg ttgatccaca tcaagtcgcg
     3841 catgctcctg cccgcggggg acgacggcgg ggacgatgag gatgacggac tcgacccgcg
     3901 cgatgaattg gtgcgccgtc tgcgcgaata ccagcgctac aaggaagcg
//
LOCUS       DASQKS010000003        17768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2101145, whole genome shotgun sequence.
ACCESSION   DASQKS010000003 DASQKS010000000
VERSION     DASQKS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17768
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101145"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..565
                     /locus_tag="VE170_00055"
     CDS             5..565
                     /locus_tag="VE170_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYJ13850.1"
                     /translation="MVLWDTAGIRDTDDQIEKLGVELSRQHLQKADALVMVIDGSVEL
                     TPEDCALLRDIGAKKAVIAVNKNDLPGVVDLHSILEKPEIKIIGTSAKSGAGIESLKI
                     ILRELILDCANESPVTVTNLRHRSELVRSADGLTRAIATLDQGLAPEFAAIDLHDARE
                     ALEEIIGVVHNEDILERIFSDFCIGK"
     gene            628..2511
                     /gene="mnmG"
                     /locus_tag="VE170_00060"
     CDS             628..2511
                     /gene="mnmG"
                     /locus_tag="VE170_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HYJ13851.1"
                     /translation="MNSKVIFDVIVVGAGHAGIEACLASARMGCTTLMMTLNLDHIGQ
                     MSCNPAIGGIGKGHLVKEIDALGGEMARAIDETGIQFRILNTKKGPAVRASRAQADKA
                     LYRMRMKRVLESCPGLTLRQGSVEALIVEDGQIKGVETQIGERFYGRKVILTTGTFLK
                     GLIHIGDRNYAAGRAGDFAVQGLSESLVGLGFKAGRLKTGTCPRLDSRTIDYSELTIQ
                     DGDHPPQPFSFSSSGVTQRQVPCHITYTNSRTHEIIRNAMHRSPMYSGVIKSRGPRYC
                     PSIEDKIHRFADKDRHQIFLEPEGLDTVEVYPNGLSTSLPLDVQVEMVRSIKGLEHAE
                     VMRHGYAIEYDYFEPTQLFPSLETKLVKGLYHAGQINGTTGYEEAAAQGIMAGINAVL
                     SLRGEAPLVFSRDQAYIGVLIDDLVTKGTDSEPYRMFTSRAEYRLLLREDNADLRLSE
                     TGHRIGLADEAAFQRFTEKKLAIDDLVGYLDSNQLMPDSETNSRLEAIGKPPLKNLTK
                     LSQILRRPEISLADIRVIFPDMTNYSRDVDSQVEVQIKYEGYVNRQNDMVGRFQKMED
                     VRLPEDIDYSQISGISREVCEKLSRIQPRSLGQASRISGITPAAMTLLSFYLKKRKSV
                     ESY"
     gene            2687..3481
                     /locus_tag="VE170_00065"
     CDS             2687..3481
                     /locus_tag="VE170_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ13852.1"
                     /translation="MSKIIAIANQKGGVGKTTTAINLAACLALEARRTLLVDIDPQGN
                     STTGLGVDRAQVAASLYHVLEGTRFVGEAMCQTEVPELLMIPATKDLVGAEVELVNTE
                     RREYRLKDALASVVAQYDYVLLDCPPSLGLLTLNALTAADSLLIPIQSEYYALEGLSA
                     LLETMNLVQKGLNPSLHLEGIVITMFDSRNRLSHQVVEEIRAHFPDKMFNAIVRRNVR
                     LSESPSYGKPICLYDPTSTGALDYRELAKELISRGERNANAEKGVG"
     gene            3453..4340
                     /locus_tag="VE170_00070"
     CDS             3453..4340
                     /locus_tag="VE170_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYJ13853.1"
                     /translation="MPMQKKGLGRGLSALIPGAPESATVETPSGSSPRQVAVDRITTS
                     PFQPRRAFDETKIDELASSIRNQGIIQPLIVRPKNDGFELIAGERRWRAAMRAGLSHV
                     PVVVRDASDHEAMQIALVENLQREDLNSIEEANGYRRLQEEFHWSQEEMAEKVGKSRP
                     AIANSMRLLSLPSEVQQEIAAGNLPAGQARALLGLHTEPLIVSTCREVIAKGLSTRDT
                     EKMVRSLLIGRRRRRSVPLLDPDLKTIVEELQRSLGTRVRLLPKARTAKGKVEIEYYT
                     AADLERIFETITKKSIVST"
     gene            complement(4463..6520)
                     /locus_tag="VE170_00075"
     CDS             complement(4463..6520)
                     /locus_tag="VE170_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYJ13854.1"
                     /translation="MAQQNTNRLIDETSPYLLQHAHNPVDWYGWGEEAFQKAKAEDKP
                     ILLSIGYSACHWCHVMERESFENEEIARLMNELFVNIKVDREERPDLDEIYMSAVQML
                     TGRGGWPMTLFLTPDRKPFYGGTYFPPEDRGGMPGFPRVLMGVNQAYRERPLDVEKSV
                     TQILGALQRMAESAATAKSFSKTVIADAAAKVAQAYDADNGGLGQAPKFPNAGVYELF
                     LRYYHHSKSSRYLEMVTHTLTKMARGGIYDHLGGGFHRYSVDAKWLVPHFEKMLYDNG
                     QLVRIYAHAFKATQEPLFKTVVDETLTYLLREMLHPEGGFYSTQDADSEGVEGKFFVW
                     TEAEVMQILGEADGAIFCRIYDVTEGGNFEEKNIIHPILTLEQASKYFRMELGEIERV
                     IADAKQKLFIEREKRIKPFRDEKIITAWNGLMLSGLAEAIKISPQPVYIDAVNRTVEF
                     IFAKLFRDGFLLHTYKDGRAKLLGYLDDYAFTAVGLLDLYEALFDRSALSRAIELAEI
                     MLREFWDEQDGGFFSTGNSHEKLISRAKPIFDASIPSGNALATQLMLRLSHITGEERY
                     FRYAEKVLRSYYDAMESQPFGFAHLLCALDFYLEKPKEVVIVGEREDRRVEELLTKIH
                     SMYQPNMTLQLARPDESLEKLSPLLQGKSQVGGNPTAYVCHNSTCSAPVTTWDGLKPL
                     LES"
     gene            complement(6534..6737)
                     /locus_tag="VE170_00080"
     CDS             complement(6534..6737)
                     /locus_tag="VE170_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYJ13855.1"
                     /translation="MTGVIKKIMRDKGFGFIKPDDSSEDVFFHRSSLAPRVQIEDINE
                     GDTVQFQTRRGDKGPVAFDLKVR"
     gene            6836..7918
                     /locus_tag="VE170_00085"
     CDS             6836..7918
                     /locus_tag="VE170_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HYJ13856.1"
                     /translation="MSSILFYITGHGFGHAIRSQQVVRALAAIRPEQRIYIRTTAPEW
                     IFYDTTVPIHYSHQAIDTGIVQTDSLHMDLTKTLAACRAIYSNQHKLIEQELAFIKAN
                     AIDLVVGDTPPLAFEIAARAGVPSVSITNFTWDVIYRAYVKSFPEFAPLIDTMTQYYG
                     KATAALTLPYPCDTAMFPRQQAIPWITRTSDLTREQARQTFNLPQAATIVLLAFGGLG
                     LDRLPWKQLARQRRYFFVTTGTSHRQERNLLTLPATQTLYQDLICMANAIVTKPGYGI
                     VADVLAQRVPVLYTDRGDFAEYPQLVAALSDCATAEFIPQQELLGGNWSPYLSRLLAK
                     PPHWPEIELNGAQVAARQIFTLLDGS"
     gene            7982..8920
                     /gene="ispH"
                     /locus_tag="VE170_00090"
     CDS             7982..8920
                     /gene="ispH"
                     /locus_tag="VE170_00090"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYJ13857.1"
                     /translation="MAIEKVILAKPRGFCAGVEMAIESVDRALAQHGAPLYVFHEIVH
                     NQNVVGNFKKKGVVFVNSIAEVPAGSNVIFSAHGVSPAIWQQAKEKHLNWVIDATCPL
                     VDKVHREVRKFVAQDYWIILVGHRDHDEITGTSGEAPQRTLVVENVAEAKAVMPPNPQ
                     KLVVLTQTTLSVDDTKEIIDVLKQRFPKMITPAKEDICYATQNRQDAVKQLALQVDLV
                     LVIGSKNSENSNQLVKVARALGKPAYLIDDYSAIDPQWLHGIATIGITSGASVPEQLV
                     QQAVEFFQRQGATVEQVGLVDENIHFALPSEVTGLQ"
     gene            8964..9356
                     /locus_tag="VE170_00095"
     CDS             8964..9356
                     /locus_tag="VE170_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ13858.1"
                     /translation="MARIVHIAVKVDDLAKATEFYEKAFGFVEVQTGQVRDHTSRHLS
                     DGAIDLALIKYDSEDSAESRAAGLGPCIHHFGIEVDDMKMSAAELAKYGCEIISAPDV
                     IPIKFRAPGGTVAEISPKARYKQTKDNS"
     gene            9375..9845
                     /locus_tag="VE170_00100"
     CDS             9375..9845
                     /locus_tag="VE170_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ13859.1"
                     /translation="MALHCKAEDIPNRQVVRSGEQGVGSMVVKRAYGQSCSLMHAVRA
                     PGYHTTPHAHAAEQLNHVLQGEIWFFVENEGFHCKAGDFQRVPSNKIHWAWNRSNADA
                     IVVEAHSPALVAGPQSEGSIGLFADGEIAAAKEPSQNNFVPFEWQNVERQIFGG"
     gene            10220..10660
                     /locus_tag="VE170_00105"
     CDS             10220..10660
                     /locus_tag="VE170_00105"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ13860.1"
                     /translation="MVAALCLALFGAYVTYVGSNLAYVSDVGPGPGFFPFWIGAGLLF
                     FACYQAIQSMSAPRRQNQSAQPSWRGSGRALAGWLAMALSIFLFGWIGFAASFVLLTI
                     FFIVVIEGRPILPALGIGVALTVIFHVLFVITLGVSLPAGPWGF"
     gene            10684..12195
                     /locus_tag="VE170_00110"
     CDS             10684..12195
                     /locus_tag="VE170_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ13861.1"
                     /translation="MDALGNLLLGFSVALAPVNLFWCFVGVVLGTIVGVLPGLGPAAT
                     IAMLLPLTLKMDHTTAIITLAGIFYGARYGGSTTSILLNMPGESSSVATCLDGYQMAR
                     QGRAGAALGISAIASFIAGTVGVLGLMLIAPPLARFALRFAPPEYFALMVLGLAMVVF
                     LAGDSMVKALLALLVGLWLATIGTDLFSAESRFTFGQAKLLGGVDFIAATVGIFAIGE
                     VLVNLQSRAGGEIVKLPRGIRNLLPTLQDLKDCRFAFVNGSLIGFFVGALPGAGATVA
                     SFLSYGLEKAVSRHPEKFGTGVIEGVAAPEGANNADAGGALVPLLTLGIPGGNTTAIL
                     LGGLILWGYKPGPLLIQEHPQLFWGLVASMYVGNVLLLVLNLPLVTLFAQILRLPSHV
                     LHPFIVGISVVGVYSVNNSLFDVWLMALFGLLGYLMRKLDFPAAALILGLVLGDVMER
                     ALRQSLMMSQGDLAIFVNRPISATLLAIAAVLLCLPALRWVRAWRCEAVAGEG"
     gene            12231..13214
                     /locus_tag="VE170_00115"
     CDS             12231..13214
                     /locus_tag="VE170_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYJ13862.1"
                     /translation="MKRFRYSLPVALAGVALLLPHPAASQEFKPSKQIETVVHTGPGG
                     GSDLFARAIAEMLQKEKLISQRMQVVNKSGGGPAVAMAYLAEKKGDSHTIGFYTGVWV
                     TNPLTTVEATVTLKDLTPVTRLVLEPAVIAVKADSPYKTVKDFIEAAKKNPKQLNQSG
                     GTVLSRDNLMRLLLQKATGAQWNFISFPSGGERLSNLLGGHVQMMVIEPQEAGEQIRA
                     GNLRVIATLTEKRLASLPNVPTIKEQGIDVPLIPQARGMLLPPAAPKEVIAYWEGVFD
                     AFVKTPSWKQYVEQNQFEDAYLKGAALNKFIDALKDQMREVLKEAGAKVIR"
     gene            13224..14591
                     /locus_tag="VE170_00120"
     CDS             13224..14591
                     /locus_tag="VE170_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ13863.1"
                     /translation="MNPTQTLARYALKLRYRDIPSEVIERAKACILDTLAVSLYGSTK
                     PWSKTVSEFVRSAGSKGNATVFGGKQKASPERAALANGTMAHAFELDNVRQPGAGVHP
                     GATAFLPAFAVAESEKTDGRALLTAFVAGCEVMSRIGVAAGNSLERRGFHAPGLTGTF
                     GAAVAAGRLLELNEKQLVNALGIAGSYSGGLIEFSRCQEGAMIKRLHLGKAAEGGVTA
                     ALLASRGFAGPESVLEGKFGFCQSFSDSPKLEYLTHRLGQEWESMNICIKRCACHINA
                     HAPIEALQNLRDELGFKPEDIRAITIGGIEKLVSHHASYQPTDLMMGQYSIPFCVALS
                     SYFDPTDPASFDAGKLKNKKIAALMAKIRLEVDHEIEEKGWDRAARLTIELADRRRES
                     KLVIHFKGTPGNPLSDAEVEAKARKLTRGIIAERQLAKLVGMVQQLEKVTDVSQIGDM
                     LRGGK"
     gene            15141..16361
                     /locus_tag="VE170_00125"
                     /pseudo
     CDS             15141..16361
                     /locus_tag="VE170_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            16459..>17768
                     /gene="glnA"
                     /locus_tag="VE170_00130"
     CDS             16459..>17768
                     /gene="glnA"
                     /locus_tag="VE170_00130"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HYJ13864.1"
                     /translation="MSVKDVLEMAKKNKVEIVDFKFIDVPGLWQHFSIPATDFDADKF
                     EQGLGFDGSSIRGFQSINESDMILVPDPDTAMMDPFTEHPTMSFVCNVLDAITRQPYS
                     RDPRYITQKAEKYLKSTGLADISYWGPEIEFFIFDSIRFDQNHHEGYYHIDSSEGFWN
                     SGNGDGPNLGNKIRYKEGYYPVPPMDQYQDLRSQMVINLEKCGIKIEVHHHEVATGGQ
                     TEIDMRFDTMTKMADKVLLYKYVVKNTAKQRGKVVTCMPKPLFQDNGSGMHCHQSLWK
                     SGKNIFYDKKGYGLISETARHYIGGLLKHAHALCAFIAPTTNSYRRLVPGYEAPINLV
                     YSARNRSACVRIPMYSTSEKAKRLEFRTPDPSANPYLAFPAMLMAGLDGIRNKIEPPK
                     PIDKNLYELEPEEAAKVKSMPGSLDQALDALEKDHDFLLKGDVFT"
BASE COUNT         3967 a         5073 c         4809 g         3919 t
ORIGIN      
        1 accgatcgta ctatgggata ccgccgggat ccgagacacc gatgatcaaa ttgaaaagct
       61 cggcgtcgaa ctatcgcgcc agcacttgca gaaagccgat gctttagtga tggtcatcga
      121 tggttccgtc gagctgacgc cggaagattg cgcgttgctg cgggacatcg gtgctaaaaa
      181 ggccgtgatt gccgtaaaca aaaatgattt gcctggggtt gtcgaccttc attcgatatt
      241 ggagaaaccg gaaattaaga taatcggcac ctcggccaag tcaggcgctg gaattgaatc
      301 gctaaagatt attttgcgtg aactaatcct cgactgtgct aatgaatctc cggtaaccgt
      361 aaccaacctg cgccatcgca gtgagttggt tcgcagtgcc gacggcttaa ctcgcgctat
      421 agcaaccctt gatcaaggtt tggccccgga attcgccgcc atagatttgc acgatgcccg
      481 cgaggccctc gaagagatca ttggcgtggt tcacaatgaa gatattttag aacgtatatt
      541 tagcgatttt tgtattggga aatagactat taaaacattt ttataagaaa ctttttaaat
      601 ttgcggcgcc ttgtttcacg tgaaactatg aactccaaag taatattcga tgtaattgtc
      661 gttggtgccg gccacgccgg catcgaagcc tgtctggctt cggctcgaat gggttgcacg
      721 acgctgatga tgaccttgaa tttggatcat atcggtcaga tgtcatgtaa tccggcaatc
      781 ggcggtatcg gcaagggcca cttggttaag gaaatcgatg cactgggcgg cgaaatggct
      841 cgggctatcg atgagacggg gatccagttc cgcattctca acaccaaaaa aggaccggca
      901 gtgcgcgctt cccgggccca agccgataaa gcgctctatc gtatgcgcat gaagcgagtg
      961 ctggaaagct gccccgggct gactcttcgg caaggcagcg ttgaagcgtt gatcgtcgaa
     1021 gacgggcaaa tcaagggagt cgaaactcag attggcgagc gcttctatgg ccggaaggta
     1081 attctgacaa ccggaacatt tttgaaggga ctcatccata ttggcgatcg aaactatgcg
     1141 gccgggcggg caggtgattt cgccgtccag ggactgagcg agtctttggt tggcctcgga
     1201 ttcaaagccg gcaggctcaa gaccggtacc tgtccacggc tcgatagtcg gaccatcgat
     1261 tattctgaac taaccatcca agacggggat cacccgcccc aaccgttctc attttcgagt
     1321 agcggcgtta cccagcgtca ggtgccctgt cacattacct acaccaacag ccgcactcac
     1381 gagatcattc gcaatgcgat gcatcgttcg ccgatgtatt ctggtgtgat caaaagccgc
     1441 ggaccaagat actgcccgtc gatcgaagac aaaatccatc gcttcgccga caaggatcgc
     1501 caccagatat ttctcgaacc ggagggattg gacacggttg aagtctatcc gaatgggctg
     1561 tcgaccagct tgccattgga tgtccaagtc gagatggtcc gttcgattaa agggctggag
     1621 cacgccgagg tcatgcgcca tggctacgcc attgagtatg actattttga gccaacccag
     1681 cttttcccca gtttggaaac taaactagtc aaggggctct atcacgccgg tcaaattaac
     1741 ggcaccaccg gttatgaaga agcggccgcc caagggatta tggcaggaat caatgcagtg
     1801 ttgagcctgc gtggcgaggc ccctttggtt ttctctcgtg accaagccta cattggcgta
     1861 ctgattgacg acttggttac caaaggaacc gatagcgagc cgtatcgcat gttcacttcg
     1921 cgggccgaat accggttgtt actccgcgaa gacaacgccg atttgaggct tagcgaaacc
     1981 ggccatagga ttggattagc ggacgaagct gcattccaga ggttcacaga aaaaaaatta
     2041 gccatcgacg atctagtcgg ttacttggat agcaaccagt tgatgccgga cagcgaaacc
     2101 aattcgcgtc ttgaagcgat tggtaaaccc ccgctgaaaa atctcacaaa gcttagccaa
     2161 attttacgca gaccagaaat ttccctggca gatatcagag tgatatttcc ggacatgact
     2221 aattactccc gcgacgtaga ttcccaggtg gaagttcaaa tcaaatacga aggctacgtc
     2281 aaccggcaga acgacatggt cggcagattc cagaaaatgg aagatgtcag gctacccgag
     2341 gacatcgatt attcccagat cagcgggatt tcacgagaag tgtgtgagaa attgagtcgc
     2401 atccaaccgc gctccctggg ccaagcatcg agaatttccg ggataacccc agcggccatg
     2461 acattgctgt ccttttatct gaagaagcgt aagagcgtcg agtcttatta gcaattacct
     2521 ctcgaataga ctagcttttt accatagccg ctctcggagc acactctccg ttgctttttc
     2581 tgtttcacgt gaaacagaga taatgctttt catccataaa taattgtggt agtacttggc
     2641 tatgctcgtt aatattttgt aagctattga tttacaatgg atttttgtga gtaaaataat
     2701 tgcaatagcg aaccaaaaag gcggcgtcgg caaaaccaca accgcgataa acttagcggc
     2761 ttgcttggcg ctcgaggctc gccggacgtt gttggtcgac atcgacccgc aggggaacag
     2821 cactaccggt ctgggcgtcg accgcgctca agtagcggct agcttgtatc acgtcctgga
     2881 agggacgcgc ttcgtcggcg aggcgatgtg ccaaactgag gtgccggagc tgctgatgat
     2941 tcccgcgacc aaggacttgg tcggtgccga agtcgagctg gtcaacaccg aacgccgcga
     3001 gtatcgcctc aaagacgcct tggcctcggt agtggcgcaa tatgactatg tgctgctcga
     3061 ctgcccgcct tctcttggtt tgctcaccct caatgcattg acggcggcag actccctgtt
     3121 gattccgatt caaagcgagt attacgccct ggagggtttg agcgcgctgc tggagactat
     3181 gaatctcgtg cagaaaggtt tgaatccgtc tctgcacctt gaaggtatcg tcatcaccat
     3241 gttcgatagc cgtaatcgcc tatcgcatca ggttgtcgag gaaattcgcg cgcactttcc
     3301 cgacaaaatg ttcaatgcca tcgtgcgccg caatgttcgc ctcagcgaga gcccaagcta
     3361 cggcaaacct atttgtttgt atgatccgac ctcaaccgga gcgttggact accgagaatt
     3421 agcaaaggag ttaattagtc gtggagaacg caatgccaat gcagaaaaag gggttgggta
     3481 gaggacttag cgcgctcatt ccaggtgcgc cggaatctgc cacagtcgaa acgccgtccg
     3541 gcagttcgcc cagacaagtt gccgtcgacc ggattaccac cagtccgttt cagccgcgac
     3601 gcgctttcga cgaaaccaag atcgacgaac tcgcatcgtc aattcgcaat caggggatta
     3661 ttcagccgtt gatcgttcgc cccaagaatg acggcttcga gttgattgcc ggcgaacgcc
     3721 gctggcgcgc cgccatgcgc gccggcttga gtcatgtgcc ggtggtggtg cgtgacgcca
     3781 gcgatcatga ggcgatgcag atcgctctag tggaaaatct ccagcgcgaa gatctcaatt
     3841 ccatcgagga agccaacggc tatcggcgct tgcaggagga atttcattgg agccaggaag
     3901 agatggccga gaaagttggc aagagccggc cggcaatcgc caactccatg cgcctgctct
     3961 cactgccgag cgaagtgcag caagagatcg cggcgggaaa tttgccggcc ggccaagccc
     4021 gcgcgttgtt gggcttgcac accgaaccgt tgatagtctc gacatgccgc gaggtaattg
     4081 ccaaaggcct atcgacccgt gacaccgaaa agatggtgcg ctccctatta attgggcggc
     4141 ggcgccggcg atcggtgccg ctactcgatc ccgatctcaa aacgatcgtc gaagagttgc
     4201 agcgatcctt gggaactcgc gttcgcctgc tgcctaaggc aaggaccgcc aaaggaaaag
     4261 ttgagatcga atattatacc gcggcggatc tcgaacggat cttcgagacg atcactaaaa
     4321 aatctattgt ttctacttaa attatcgttt taatattaag aaagatttat catccgtggc
     4381 cgcatcaaag ggaagctgtc taatccgatc agccctcctg ccacgctcgg ctcgacggct
     4441 gttctaccgc gaataatccg ggctagcttt caagcagtgg tttcaaacca tcccaggtcg
     4501 tcaccggcgc cgaacaggta gaattatggc aaacgtaggc agtcggattt ccaccgactt
     4561 gggatttccc ctgcaacaag ggcgagagtt tctccagcga ttcatcggga cgcgccagct
     4621 gaagcgtcat attcggttgg tacatggaat ggatcttcgt cagcaattcc tcgactcttc
     4681 gatcctctcg ttcgccgaca atcacgacct ctttgggttt ctccaagtag aaatccaacg
     4741 cgcataacaa atgcgcgaac ccgaacggct ggctctccat ggcgtcatag taagagcgca
     4801 gcactttttc cgcgtatcga aaatagcgct cctcgccggt gatgtgggac aagcgcagca
     4861 tcagctgcgt cgccaaggcg ttgcccgagg gaatcgacgc atcgaagatc ggtttggccc
     4921 ggctgatcag tttctcgtgg gaattgccgg tggaaaaaaa cccgccgtcc tgttcgtccc
     4981 aaaactcccg cagcataatt tcagccaatt caatcgcccg cgacaatgcc gaacggtcaa
     5041 acagcgcctc atagagatcg agcaaaccga ccgccgtgaa tgcgtagtca tcgaggtagc
     5101 ccaacagctt cgcccggccg tctttgtagg tgtgcaaaag aaaaccgtcg cgaaataatt
     5161 tggcaaagat aaactcgacg gtgcggttca ccgcatctat atatacgggc tgcggagaaa
     5221 tcttgatcgc ctccgccagg ccggacagca tcagaccgtt ccatgcggtg ataattttct
     5281 cgtcgcgaaa cggcttgata cgtttttcgc gctcgatgaa cagtttttgc ttcgcgtcgg
     5341 cgatcacccg ttcgatctca cccagctcca tgcgaaagta cttgctcgcc tgctctaagg
     5401 taagaatcgg atggataata tttttctcct cgaagtttcc gccctctgtg acatcgtaaa
     5461 tccggcaaaa gatcgcgccg tcggcttcac cgagaatttg catgacctcg gcttcggtcc
     5521 agacgaagaa ttttccttcc acgccctcgc tgtcggcgtc ctgggtcgaa taaaaccctc
     5581 cttcagggtg gagcatctcg cgcagcaaat aagtcaaagt ttcgtcgacg acggttttga
     5641 acaacggttc ctgggtcgcc ttgaacgcgt gagcataaat gcgcaccagc tggccattgt
     5701 cgtagagcat tttttcaaaa tgcggcacca accatttggc atcgaccgaa tagcgatgaa
     5761 agccgccgcc gagatggtcg tagatcccgc cacgagccat tttcgtcagc gtatgggtaa
     5821 ccatttccag atagcgcgaa ctcttcgaat gatggtaata gcgcaagaac agttcataga
     5881 ctccggcatt ggggaacttt ggcgcttggc caagcccgcc gttatccgcg tcgtaagcct
     5941 gggcaacttt cgccgccgcg tcggcgatga ccgtcttgga aaatgatttc gcggttgccg
     6001 cggactccgc catgcgttgc aatgcgccga gaatctgcgt gacactcttt tcaacatcca
     6061 gcgggcgttc gcgataggcc tgattcactc ccatgagcac ccgcggaaag cccggcatgc
     6121 cgccacggtc ttccggcggg aaataggtac cgccgtagaa cggcttgcga tccggtgtca
     6181 gaaacagcgt catcggccaa ccgccgcggc cggtgagcat ttgcaccgcg ctcatataaa
     6241 tctcatcgag atccggccgc tcctcccggt cgactttaat attgacgaac agctcgttca
     6301 tcaagcgcgc aatttcttcg ttttcgaaag actcgcgctc catcacatga caccagtgac
     6361 aggcggaata gccgatgctc agcagtatcg gtttatcttc cgccttggct ttttggaacg
     6421 cctcctcacc ccaaccatac caatccaccg gattgtgcgc gtgttgcagc aaatacgggc
     6481 tggtctcgtc gatcaggcga ttggtatttt gctgtgccat cgaaatcccg ggctcagcgc
     6541 actttcaagt cgaacgccac cggacccttg tcgcccctgc gggtttggaa ctgcaccgtg
     6601 tcgccttcgt tgatatcctc gatctgcacc cgcggcgcta aactactgcg gtgaaagaaa
     6661 acatcttcgc tgctatcgtc cggcttgata aatccgaaac ctttatcgcg catgattttc
     6721 ttgattaccc cggtcatcgt atcacctttt ccctgcgcgt cagcgcagcg ctggcttgac
     6781 aatccctgag ccactcccta gtttgagctt gcagtttcat acaactaaaa gccgcttgag
     6841 ttcaattctc ttctatataa ccggtcacgg cttcggtcac gccatccgtt cgcaacaagt
     6901 cgtgcgggcc ctggcggcga ttcgcccgga gcagcgtatt tacatccgta ccaccgcgcc
     6961 ggaatggatt ttttacgaca ccaccgtgcc gatccactat tcgcaccagg ccatcgatac
     7021 cggcatcgtc caaaccgaca gcctgcacat ggatttgact aaaactttag ccgcctgtcg
     7081 cgcgatttac agcaaccagc ataaacttat cgaacaggaa ctggcattca taaaagccaa
     7141 cgccatcgat ctagtcgtcg gcgacacacc gccgctcgct tttgagatcg ccgcccgagc
     7201 gggtgtgccg tctgtctcca tcaccaattt tacctgggat gtgatctacc gcgcttacgt
     7261 aaaatcattc cccgaattcg cgccgttgat cgacaccatg acgcaatatt acggcaaagc
     7321 caccgctgcg cttaccctac catatccctg cgataccgcc atgtttccgc gccagcaggc
     7381 gattccatgg atcacccgaa catccgattt aacccgggaa caggcacgcc agacattcaa
     7441 cctgccacag gcagcaacca tcgtgttgct cgccttcggc ggcctgggtc tcgatcgttt
     7501 accatggaaa caactggcac ggcagcggcg atactttttc gtcaccaccg gaacaagcca
     7561 tcgacaggag agaaatcttc tcacattgcc ggcaacccaa actctctacc aagacctaat
     7621 atgcatggcc aacgccatcg tcactaaacc cggctacggc atcgtcgccg atgttttggc
     7681 gcagcgcgtg ccggtcctct acaccgaccg cggcgacttc gccgaatatc cgcagctggt
     7741 agcggccttg agcgactgcg ctacggcgga attcattcca cagcaggagc tcctgggcgg
     7801 caattggtcg ccttatctga gccgtttgct ggccaaaccg ccgcattggc cagagatcga
     7861 attgaacggc gctcaggttg ctgcgcggca gattttcacg ctgttggatg ggtcgtgatt
     7921 atccggtgcg cgctacgagt tgacagtgcc ggccgactca gccagacttg aaaacaactt
     7981 tatggccatt gagaaagtga ttctcgccaa gccgcgcggc ttttgtgccg gcgtcgaaat
     8041 ggcaatcgag agcgtcgatc gcgccttggc acaacatggc gcgccgctct acgtgtttca
     8101 tgagatcgtc cacaatcaga atgtcgtcgg taacttcaaa aaaaaaggcg tcgtgttcgt
     8161 caactcgatt gccgaagtac cggcgggatc caatgttatc ttcagcgccc acggcgtctc
     8221 gccggcaatt tggcagcagg ccaaagaaaa acacttgaac tgggttatcg atgccacctg
     8281 tcctttggtc gacaaagtcc accgcgaggt gcgcaaattt gtcgcccaag actattggat
     8341 aatcctggtc ggccaccgcg accatgacga aataaccggc accagcggcg aagctcccca
     8401 gcgcaccctg gtcgtcgaga acgtcgccga agccaaggcg gtcatgccgc cgaatccgca
     8461 gaaactcgtc gtcctgacgc aaacgacatt gagcgtcgat gataccaaag aaatcattga
     8521 cgtcctcaaa cagcgctttc ccaagatgat cactccggcg aaggaggaca tatgttacgc
     8581 cacgcaaaac cgccaggacg cggtcaagca attggcgctg caagtcgacc tggttctggt
     8641 catcggttcg aagaatagtg aaaactcaaa tcagctggta aaagtcgctc gcgccctggg
     8701 caagccggca tacctcatcg acgattacag cgctatcgac ccgcaatggt tgcatggcat
     8761 tgccacaatc ggcatcactt ccggcgcgtc ggtgccggaa caactggtcc aacaggccgt
     8821 ggagtttttc caacgccaag gcgcaactgt ggaacaagtt ggcttggtcg acgaaaatat
     8881 ccattttgcc ctacccagcg aggttaccgg actacagtaa ccgataaccg gcgggcgatt
     8941 tccatcgtaa tcaaggagtc cccatggcac gcatcgttca catcgccgtc aaagtcgacg
     9001 atttagcaaa ggccacggag ttttacgaaa aggcttttgg tttcgtcgaa gtgcaaaccg
     9061 gccaagttcg cgaccatacg tcgcgccacc taagtgacgg cgccattgac ctcgcgctga
     9121 tcaaatacga cagcgaagat tcggccgagt cccgcgcggc cggtcttggc ccgtgcatcc
     9181 atcacttcgg catcgaagtc gacgacatga aaatgtccgc cgccgaactg gcaaaatacg
     9241 gttgtgaaat tatcagcgca ccggacgtga tcccgatcaa attccgcgcc cccggcggca
     9301 ccgtcgcgga aatctcgccc aaagcgcgct acaagcaaac aaaagacaat tcttaaaacc
     9361 aaggagcatc gacaatggcg cttcattgca aggccgaaga tattcccaat cgccaggtcg
     9421 ttcgcagcgg cgaacagggc gtcggatcga tggtggtcaa acgggcctat ggccagtcat
     9481 gcagcctgat gcatgccgtg cgggcacccg gctatcacac cacgccccat gcccatgccg
     9541 ccgagcagct caatcatgtg ctccaaggcg agatttggtt cttcgtggaa aacgaagggt
     9601 ttcattgcaa ggccggcgac ttccagcggg tgccgtcgaa caagattcac tgggcgtgga
     9661 accgttccaa cgccgacgcc atcgttgtcg aggcccattc gccggcgttg gtcgccgggc
     9721 cgcagagcga aggctccatc ggtttgttcg ctgacggcga aatcgccgca gccaaagagc
     9781 cgagccaaaa caacttcgtt cccttcgagt ggcaaaacgt cgagcggcaa atattcggcg
     9841 gctagtcatt cccagagcgc caagcctcgc tatattttaa accggaattc cggatatcgc
     9901 ctaagcatcc cacccggcgc ggagagcgtt tggagcgtga agcgccaaac tgcacgggcg
     9961 caaagtcaga ctttactcgc atggcaaact tactcctatt tcccgcgctg accggacgca
    10021 cctaagtcac ataaaccctc agagataggg acccatcgcc ttcatcgcag agggcccgct
    10081 tctcgccgcc tcctcgctgc caactaggaa tatcagcgaa agcgattatc gatagacaaa
    10141 ttcccttgtg aaaaacctcg ttcagggata ataaccgcac tcacagccga tgcgccccgg
    10201 gattcgagat agaagtggtt tggttgccgc gctctgttta gcgttattcg gcgcctatgt
    10261 cacctacgtc ggctcgaacc tcgcctatgt atcggatgtc ggtcccggtc ccggtttctt
    10321 tccgttctgg atcggcgccg gcctgctatt ctttgcctgc taccaagcga ttcaatccat
    10381 gtcggcgccg cgacggcaaa accagtcagc ccagccaagt tggcgcggct ccggtcgcgc
    10441 cctcgccggc tggctcgcga tggcgctctc gatttttctt ttcggctgga tcggcttcgc
    10501 cgccagtttc gtgctgctga cgattttttt tatcgtcgtg atcgaaggcc ggcctatctt
    10561 gccggcgctt ggcatcggcg tagctctaac tgtcatcttt catgttttgt ttgtcatcac
    10621 cctcggggtt tcattgcccg ccggcccctg gggtttctga gttgctaaac gactagcgca
    10681 atcatggacg cactgggcaa tctcctgctt ggcttttcgg tggcgctggc accggtcaat
    10741 ctattctggt gttttgtcgg cgtggtgctt ggcaccatcg tcggtgtcct gcccggcttg
    10801 ggaccagccg ccaccatcgc catgctgctg ccgctcaccc tcaagatgga tcacaccacg
    10861 gcgatcatca ccttggcggg gattttttac ggcgctcgct acggcggctc gacgacgtcg
    10921 attttactca acatgccggg cgagtcgtcg tcggtggcga cctgtctcga cggctatcag
    10981 atggcgcgtc agggccgggc cggcgccgcg ttgggcatct ccgccatcgc ttcgtttatc
    11041 gccggcaccg tcggggtgct cggattgatg ttgatcgcgc cgccgctggc acgcttcgct
    11101 ttacgtttcg ccccgccgga atattttgcc ctgatggtcc tgggcctcgc catggtggta
    11161 tttcttgccg gcgattccat ggtcaaggct ttgctggccc tgctcgtcgg tttgtggctc
    11221 gctaccatcg gcaccgactt gttcagcgcc gaatcgcgct tcaccttcgg tcaggcaaag
    11281 ctgcttggcg gcgtcgattt tatcgccgcc accgtcggca ttttcgccat tggcgaagta
    11341 ctggtgaatt tacaatcccg tgccggcggc gagatcgtca aattaccgag gggaatcaga
    11401 aacctgctgc cgacgctgca ggatctcaag gactgccgct tcgccttcgt caacggttct
    11461 ctgatcggct tttttgtcgg cgcgctaccc ggcgccggcg ccaccgtcgc gtcattctta
    11521 tcctacggtc tggaaaaagc cgtatcgcgc catccggaaa aattcggcac cggcgtcatc
    11581 gaaggggtcg ccgctcccga aggcgccaac aacgccgacg ccggcggtgc gctggtaccg
    11641 ttactcacgt tgggaatacc cggcggcaac accacggcga ttctgctcgg cggcctgatc
    11701 ctctggggct ataaacctgg cccgctgcta atccaggaac atccgcaact gttttgggga
    11761 ttggtggcga gcatgtatgt cggcaacgtc ctgctcttgg tcctcaatct gccgctggtg
    11821 acgttgttcg cgcagatttt acgtctgccc agccatgtgc tccatccgtt catcgtcggc
    11881 atctcggtcg tcggcgtcta cagcgtcaac aacagcctgt tcgatgtttg gctgatggcg
    11941 ctgttcggcc tcctgggcta tttaatgcgc aagctcgatt ttcccgccgc ggcattgatc
    12001 ttaggcctgg ttttgggcga tgtaatggaa cgggcgctgc gccagtcttt gatgatgtcc
    12061 cagggcgatc tggcgatttt tgtaaatcgg ccgatctcgg cgaccttgtt ggcgattgct
    12121 gcagtgctat tgtgtcttcc cgcgctccgc tgggtccgcg cttggcgttg cgaggcggtc
    12181 gcaggagaag gttaaaaaaa ctgacatccg aaccggaagg agtcccaccg atgaaacgat
    12241 tccgttattc attgcccgtc gcactggctg gcgtcgcttt acttttaccc catcccgccg
    12301 caagccaaga attcaaaccg agcaagcaga ttgaaaccgt ggtccacacc ggccctggcg
    12361 gcggctcgga tcttttcgcc cgcgccatcg ccgagatgtt gcaaaaggag aaactgattt
    12421 cccagcgcat gcaggtggtc aacaaatccg gcggcgggcc ggccgtggcg atggcgtacc
    12481 tcgccgagaa aaaaggcgat agccacacga ttggtttcta cacgggggta tgggtgacca
    12541 atccattgac gacggttgaa gcgaccgtaa cgttgaaaga tttgacgccg gttacccgtt
    12601 tggttttgga gccggcagtg atcgcggtca aggccgactc gccctataag accgtgaaag
    12661 atttcatcga agcggcgaaa aagaatccta aacagctcaa tcaatccggc ggcacggtcc
    12721 tttcacgcga caatctcatg cgcctgctcc tgcaaaaagc caccggcgcc cagtggaact
    12781 ttatttcctt cccgagcggc ggtgaacggt tgtccaattt actcggcggc catgtccaga
    12841 tgatggtcat cgaaccgcag gaggccggcg aacagattcg cgccggcaac ctgcgcgtaa
    12901 tcgcgacgct cacggaaaag cgtctggcgt cgctgccgaa tgtgccgacc atcaaagagc
    12961 agggcatcga tgtgccgttg attccccagg cacgcggcat gcttttgccg ccggccgcgc
    13021 caaaagaagt tatcgcctat tgggaaggcg tgttcgacgc tttcgtgaaa accccgagct
    13081 ggaaacagta tgtcgaacag aaccagttcg aagatgccta cctcaaaggc gcagcgctca
    13141 acaaattcat cgacgccctc aaagatcaaa tgcgcgaagt gctcaaagaa gcgggcgcca
    13201 aagtgattcg ctaaggcaag gccatgaatc caacccagac cctggcgcgt tacgcgctca
    13261 aactgcgcta ccgcgacatt cccagcgaag tgatcgagcg cgccaaggcg tgcatcctcg
    13321 atacgctggc cgtctcgctc tatggctcga ccaaaccgtg gagcaagacc gtcagcgaat
    13381 ttgtccgtag cgccggctcg aaaggtaacg ccaccgtctt cggcggcaag caaaaagcgt
    13441 caccggaacg tgccgccctc gccaatggca cgatggcgca tgccttcgaa ctcgacaatg
    13501 tccgccaacc tggcgcgggg gttcatcccg gcgccacggc ttttttaccg gcctttgccg
    13561 tggcggaaag cgaaaaaacc gatggcaggg cgttacttac agcattcgtc gccggctgcg
    13621 aagtgatgtc gcgcatcggc gtcgccgccg ggaattcgct tgagcggcgc ggttttcatg
    13681 cgcccggctt gaccggcacc ttcggcgcgg ctgtcgccgc cggccggctg ttagagctga
    13741 atgagaaaca gctggtcaac gccttgggca tcgccggttc ttattccggc ggcttgatcg
    13801 aattctcgcg ctgtcaggaa ggcgccatga tcaagcggct ccatcttggc aaggccgccg
    13861 agggcggtgt caccgcggcg ctgttggcca gccgcggctt cgccggtcct gaaagcgtgc
    13921 tcgaaggaaa gttcggtttc tgccagagct tttccgattc tcctaagctc gaatatctaa
    13981 ctcaccgctt ggggcaggaa tgggagagca tgaacatctg catcaagcgt tgcgcctgtc
    14041 atatcaacgc ccacgcgccg atcgaagcgc tgcaaaatct gcgcgatgaa ctcggattca
    14101 agccagaaga cattcgcgcc atcaccatcg gcggcatcga gaaactggtg agccatcacg
    14161 ccagctacca acccaccgat ctgatgatgg gccagtatag cattcccttt tgcgtcgcct
    14221 tgagcagcta tttcgatccc accgatccgg cgagcttcga cgccggcaaa ctcaagaata
    14281 aaaagatcgc cgcgctgatg gctaagattc gccttgaagt ggatcacgaa atcgaagaaa
    14341 aaggctggga ccgcgccgcg cggctgacca tcgaactcgc cgaccggcga cgtgaatcga
    14401 agttggtcat ccatttcaaa ggcacgccgg gtaatccact cagcgacgca gaggtagaag
    14461 ccaaagcgcg caaacttacg cgcgggatta tcgccgagcg gcaattggca aagctcgttg
    14521 gcatggtcca acagctggaa aaagtcactg acgtttccca gatcggcgac atgttacgcg
    14581 gcggaaaata aaaatcgcca ccatcgaaag taagcgctga attgtaccgg taaaagaaat
    14641 ttccggcggt gcgccggtaa tcttgaccct tgcaaatctg tcatctccaa caatggcgtc
    14701 gtgacctcgc cgaggcccat gaaaaatctc aagatgcggt catggcgcgg tggtgattgt
    14761 cctagtcttc tgtgcatgca taaagtttaa gcagctgctt gaaatttagg cagtagcttg
    14821 gtcgtcgagc atccgtattc cagtttaagt atctgattta tttgtgaaaa tttttttgcc
    14881 ttggtggcat aacggttgct cctcaaaaga aatatcggat tgtttggctt agcgcctaat
    14941 attcttgcaa caaaccagga gggtcggtcg tgcagcagcc aaataaattc caatcactat
    15001 tatttgtcct acctgtgctt ctggcattgt ggtggacaac cacgacgcaa gctcaagaga
    15061 ctccgtccgc gcccgtcccg gcagtaagcg cgccggctcc aaccccacct cctacaccaa
    15121 gcgcaccggc gccagcgcca aaggtcgaca agggcgacaa cgcctggatg ctgacatgcg
    15181 ccgccctggt tttgatgatg acgatccccg gcctatttct tttttacggc gggctggtgc
    15241 gcagcaaaaa tgttctcggc acgatcatgc acagtttcat cttggtcggg gtggtgacga
    15301 tccagtggat actctggggt tattcagtcg ctttcggccc tgacgttggc ggtctatacg
    15361 gttctctcgc ctgggtcgga ttgaacgggg tcggggtcga gcccaacgcg gattatgccg
    15421 ccaccatccc gcacatgact ttcatggttt atcagatgat gttcgccatc atcactccag
    15481 cattgatcac cggtgccttc gccgagcgcg ctaaatttag caccttccta gtcttcatga
    15541 ttttatggac gactatcgtc tacgatcctc tggcccattg ggtctggggc gtcaaaggct
    15601 ggatgcgtga gatgggcgct ttggattttg ccggcggcac ggtggtacat attagctcgg
    15661 gggcatcggc cttggtggca gcgatcatgt tcggccgccg cattggctac ggccaggagg
    15721 cgatgccgcc acataatctg ccgttcagtg ttataggagc tggacttctc tgggtcggct
    15781 ggtttggttt taatgccggc agtgcgctgg ccgccgatgg tttggcgaca tccgcttttg
    15841 tcgcgaccca tgccgccgcc gccgccgctg ccctggcctg gctggtgatg gactggttgc
    15901 gcggcaaacc gactatgctc ggtgccgcga gcggtgcggt cgcgggacta gtcgcaatca
    15961 caccggcggc agggttcgtt agcccgctgt cggcgctggt gattggcgcc ggcggcggcg
    16021 tcttgtgctc cattgcttgc agcctgaaac caaaatttgg ttacgacgat tcactcgatg
    16081 tcgttggcgt gcatggggtc ggcggcacct ggggagcctt agcgaccggg ctattcgctt
    16141 ctaaagcggt caacgccgcc ggcaatgacg gtcttttctt cggcaatccc gggcagcttt
    16201 ggacgcagtt cattagcgtc gtcgtaacca tcgtattcgc catggtcgtt acggcgatta
    16261 ttctagcgat cctaaaagcg accatgggcc tgcgcgtcgc cgacgatgaa gagcgcatgg
    16321 gtttggattt gactcagcat aatgagcgcg cttacgaata aaccgagcgc aacgggacta
    16381 gttctcgctg gaagaatatg ataactggtg tgcgttttcc aaaacaaaat ctaacattcg
    16441 gatgagagga gcagatagat gtccgtaaaa gacgtcctgg agatggcgaa gaagaacaag
    16501 gtggagatag tcgatttcaa gtttatcgat gtgcccgggc tgtggcagca tttttcgata
    16561 cccgcaaccg atttcgacgc cgataagttc gaacaagggt tgggctttga cggttcgagc
    16621 attcgcggtt ttcagagcat caatgaatcg gacatgatcc tggtgcccga tcccgacacc
    16681 gccatgatgg atccgttcac cgaacacccc accatgagct ttgtctgtaa cgtgttggat
    16741 gcgatcaccc gccagcccta ttcccgcgat ccccgctata tcacccagaa agccgagaag
    16801 tatttaaagt ccaccggctt ggccgacatc agctactggg gtcccgagat cgaattcttt
    16861 atcttcgaca gtatccgctt cgaccagaac caccatgaag gctactatca catcgactcc
    16921 agcgaagggt tctggaactc cggcaacggc gacggcccca atctgggcaa caagattcgt
    16981 tacaaagaag gttactaccc ggtgccgccg atggatcagt atcaagacct gcgctcccaa
    17041 atggtgatca acttagagaa gtgcggcatc aagatcgaag ttcatcacca cgaagtcgcc
    17101 accggcggcc agaccgagat cgatatgcgc tttgacacca tgaccaagat ggccgacaag
    17161 gtgctgctgt acaagtatgt ggttaagaac accgccaagc aaagaggcaa agtggtcacc
    17221 tgtatgccca agccgctgtt tcaggacaac ggttccggca tgcactgtca ccagagcttg
    17281 tggaagtctg ggaagaatat tttctacgac aagaagggct atgggctgat cagcgagacc
    17341 gcccgacact acattggcgg cctgctaaag catgcccacg cgctgtgcgc cttcatcgcc
    17401 ccgacgacca actcctaccg cagactggtg cccggctacg aagcgccgat caatttagtc
    17461 tactcggcga gaaaccgcag cgcctgtgtg cgtattccca tgtactcgac cagcgagaag
    17521 gccaagcggc tggagtttcg cacccctgac ccgagcgcca atccctatct cgcctttccg
    17581 gccatgctga tggcggggtt ggacgggatt agaaacaaga tcgagccgcc gaaaccgatc
    17641 gacaagaatt tatacgaact ggagcccgaa gaggccgcca aggtgaagtc gatgccgggg
    17701 tcgcttgacc aggcgctcga tgccttggag aaggatcatg atttcctgct caagggcgat
    17761 gtcttcac
//
LOCUS       DASQKS010000004         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2101489, whole genome shotgun sequence.
ACCESSION   DASQKS010000004 DASQKS010000000
VERSION     DASQKS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101489"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VE170_00135"
     CDS             complement(<1..1261)
                     /locus_tag="VE170_00135"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ13865.1"
                     /translation="MTTDAAPEAAGKLAALGYRDFRLFWSGQLISNIGTWMQMTATSW
                     LLYQLTGSAVLLGLNGIFRAVPALTLGLISGTFADRCDRRWMLLWTQVILGSLSLAIG
                     VLDDVGHIQPWHIYSFTFLSAAVGAFDGPARQAMFPALVPRSALPNAVALNSLLWKGS
                     ALIGPSLGGVAISLMGTAGAFYANALSFLVVVITLLMLRVTAKAAAKRQRHFMAETQE
                     GFKYIVARPIILGITIMEAVASVFGLDHTMLTIYASDVLRVGADGFGLLQSARGLGAV
                     IGSGLFLAVGQKPYQGNILIVTAILYGIFFGLFGLAPTFAIALALMTLIGLTDTVWGA
                     ARGTIMQMITPDNFRGRVMGVFQLSNRGLHPLGQVESGLLIPLIGVRETTLFGGILVT
                     VVTLLTAWKVPAIAQFRWDDAKYKSLAE"
     gene            complement(1469..2494)
                     /locus_tag="VE170_00140"
     CDS             complement(1469..2494)
                     /locus_tag="VE170_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ13866.1"
                     /translation="MRPIRAIGPILLALALFASNATAAEPANFYQGKTVRIVVGLAAG
                     GGYDLYARTLARHLGKHIPGNPAVVVENMTGAGSIIAANYLYKIAKPDGLTIGHYLGG
                     IALQQLLGKSGIEFDALRFKYIGVPAQDSFIIGVHKTTGITDVHAWIASKQLIKFGGI
                     GPGAGSDDIPKILAATINLPAQVVAGYKGTAETRLAFNNGEVQATSNAWESTKSTWRN
                     ELESGTLKVVLQATLKSHPELKQIPVAYELAKTDEAKKLMATVLRANSPTVRPFMAPP
                     ATPNDRVQLLRKAFMDTWKDPELVAEAKKARLDIDPADGAELEQNIKEIFKLEPSQIA
                     KLKEILK"
     gene            complement(2535..3560)
                     /locus_tag="VE170_00145"
     CDS             complement(2535..3560)
                     /locus_tag="VE170_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ13867.1"
                     /translation="MLRIAILVATFLFTFTLGSGDRATAQSNFYGGKTIRFVVGFTAG
                     GGYDAYTRAIARHMGKHIPGNPTMVVDNMPGAGSMISANYTFKVAKPDGLTIGHFIGG
                     LFLQQLLGKPGIEFDSAKFEYVGVPAQDNFIFGVHKSTGITDVNGWLASKQVVKFGGV
                     ANGSGSDDIPNILKATLGLPIQLVSGYKGTADVRLAFNSGEVQGISNSWESTKSTWRK
                     EIDSGDMRIILQATLKSHPDFAKLPVAYEMAKGDEAKRLIATVLRVHGPTVRPYALPP
                     RTPKERVQILRKALMDTMKDPEFLAEAKKANLDINPEDGATLESNVKEILKLEPVLIA
                     KLKEILK"
     gene            complement(3594..>4079)
                     /locus_tag="VE170_00150"
     CDS             complement(3594..>4079)
                     /locus_tag="VE170_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13868.1"
                     /translation="LVTGYKGTAEIRLAAESGELAGGCWTWDSIRSTWSKAIQSGEAI
                     VVLQAVAKPHPDLPNVPLAQSLAKTDEARQLLQAGVQDPADFYRPYVAPPRTPKQRVD
                     ALRQAFAATMKDGEFLADAKKANLDIEPITGQEMAKLVAGIFKLNPALAAKLNNILNP
                     S"
BASE COUNT          795 a         1115 c         1291 g          878 t
ORIGIN      
        1 attctgctag ggatttgtat tttgcatcat cccaacggaa ctgggctatc gccggcactt
       61 tccaggcggt taagagcgtc accacggtga ctaagatacc gccgaacagg gtagtctcgc
      121 gcacaccgat gaggggaatc agcaagcctg attcgacttg acccaatggg tgcaggccgc
      181 ggttgctcaa ttgaaacacg cccatcaccc ggccgcgaaa attatccggt gtgatcatct
      241 gcatgatggt gccgcgcgcc gcgccccaga cggtatcggt gagaccgatc aaagtcatca
      301 gcgccagggc gattgcgaag gttggcgcca agccgaacag cccaaagaat atgccgtaca
      361 ggatcgccgt gacgataagt atattcccct ggtagggttt ttggccgacg gcgagaaaca
      421 gcccggagcc gatcaccgcg ccgaggccgc gcgccgattg caacaaaccg aacccatcgg
      481 cgccgacgcg cagcacgtcg ctggcgtaga tcgtcagcat ggtgtggtcc aggccgaaca
      541 ccgacgccac cgcctccatg atcgtgatgc cgaggatgat cggccgggcg acaatgtatt
      601 taaatccttc ctgggtctcg gccatgaaat ggcgctggcg cttggctgcc gctttcgccg
      661 tgacgcgcag catcagcagc gtgatgacga cgaccaagaa gctcagcgcg ttggcgtaaa
      721 acgcgccggc ggtgcccatc aggctgatgg cgacgccgcc gagggaaggg ccgatcagag
      781 cggagccttt ccagagaagc gagttgaggg cgacggcatt gggcaatgcc gagcgtggca
      841 ccagcgccgg gaacatcgcc tggcgcgccg gcccgtcgaa ggcgcccacc gctgcgctca
      901 agaatgtgaa gctgtagata tgccacggct ggatatggcc gacatcgtct aggactccaa
      961 tggcgaggga aagcgagccg agaattacct gagtccacaa aagcatccag cgccgatcgc
     1021 agcggtcggc aaaggtgccg ctgatcagcc cgagagtgag cgcggggacc gcgcgaaaga
     1081 tgccgtttaa gccaagcaac accgccgagc cggtgagttg gtagagcaac cagctggtcg
     1141 ccgtcatctg catccaggtg ccgatgttgg agatcaattg gcccgaccag aacaggcgga
     1201 agtcgcggta gccgagggcg gcgagtttgc ctgcggcttc aggcgcggcg tcggtggtca
     1261 tgggtgtatt gccaagatcg tagagtccgc ggagtctctg gcgcgaattc cttcctctca
     1321 ataccgcgcc tgaaaaatag cgacgaggct cagcctatcg accctggtgt aagaaatcga
     1381 gtgggcgatg aaccggggcg actttgaaaa gcggaattat tccacgggcg gaagatggag
     1441 tggcgcggcg acgaggaacg cgcgactgtc acttgaggat ttctttgagt ttggcaatct
     1501 gtgacggctc aagcttgaaa atttctttga tgttttgttc gagctcggcg ccgtcggcgg
     1561 gatcgatatc gaggcgggcc tttttggctt cggcgactaa ctcgggatcc ttccaggtgt
     1621 ccatgaacgc tttgcgcagg agctgcacgc ggtcgttggg tgtggccggc ggcgccatga
     1681 agggacggac cgtcgggctg ttggcgcgca gcaccgtcgc catcagtttt ttcgcttcat
     1741 cggttttcgc caactcgtag gcgacgggaa tctgtttcaa ttcgggatgg gacttgaggg
     1801 ttgcttgcag cactactttc aaggttcccg actcgagttc gttacgccag gtcgatttag
     1861 tcgactccca agcgttgctg gtcgcttgga cttcgccgtt gttgaaggcg agccgggttt
     1921 ccgccgtgcc cttgtagccg gcgaccactt gcgccggcag gttgatggtg gcggcgagga
     1981 tcttggggat atcgtcgctg ccggcgccgg gaccgatgcc gccgaacttg atcaactgtt
     2041 tcgaagcgat ccatgcatgg acatcggtga tgccggtggt tttgtgcacg ccaatgatga
     2101 aactatcctg cgccggcacg ccgatatact tgaatctcag cgcgtcgaat tcgattcccg
     2161 atttgcccaa caactgttgc agcgcaatgc cgcccaagta atggccgatg gtgagaccgt
     2221 cgggcttggc gatcttgtaa agatagttgg cggcgatgat cgagccggcg ccggtcatgt
     2281 tctcgacgac caccgccggg ttcccaggga tatgtttgcc gaggtgacgc gccagtgtgc
     2341 gggcgtagag atcgtagccg ccgccggcgg cgaggccgac gacgatgcgg acggttttgc
     2401 cttggtagaa gttggctggc tcagccgcgg tagcgttgct ggcgaaaagc gccagcgcta
     2461 acaagatcgg accgatcgca cgaattggcc tcatattttc tctcttctct agattctcgt
     2521 tatgaacagc aagcctactt caaaatctcc ttcagcttgg cgattaatac cggctcgagc
     2581 ttcaagattt ctttgacgtt actttcgagc gtagcgccgt cttccgggtt gatatcgaga
     2641 ttggctttct tggcctcggc gaggaactcg ggatctttca tcgtgtccat gagcgctttg
     2701 cgcaggatct gcacgcgctc cttgggagtg cgcggcggca gtgcataggg gcgcactgtc
     2761 gggccgtgga ctcgcaatac cgtggcgatc agccgtttgg cttcgtcgcc tttcgccatt
     2821 tcgtaggcga ctggaagttt ggcaaagtcc gggtgggatt tgagcgtagc ctggagaatg
     2881 attctcatgt cgccgctgtc gatttccttg cgccaggtgg atttcgtcga ctcccaggag
     2941 ttgctgatac cctgcacttc gccgctgttg aacgccaagc gaacatcggc ggtgccttta
     3001 tagccggaga ctaattgaat cggcagaccc aatgtcgctt tgagaatatt cgggatatcg
     3061 tcactgccgg agccgttggc gacaccgccg aattttacca cttgcttgct ggcgagccag
     3121 ccgtttacgt cggtgatgcc ggtggattta tgcacgccga atatgaaatt atcctgcgct
     3181 ggcacgccca cgtactcgaa ttttgccgaa tcgaattcga tgcccggttt gccgagcagc
     3241 tgttgcagga acaatccgcc gataaagtgg ccgatggtga gaccgtcggg cttggcgact
     3301 ttgaaggtgt agttggccga aatcatgctg ccggcgcccg gcatgttgtc gacgaccatg
     3361 gtcgggttgc cgggaatatg tttgcccata tggcgggcga tggcgcgggt ataagcgtcg
     3421 tagccgccgc cggcggtaaa gccgaccacg aagcgaatgg tcttgccgcc gtaaaagttg
     3481 ctttgagcgg tggcgcggtc gccagagcca agcgtaaagg tgaataaaaa cgtggcaacc
     3541 aaaattgcga ttcttagcat gatggccttt ctgattttat tgacgatatc taattaactg
     3601 ggattcaaaa tattattcag tttcgccgcg agagccgggt tgagcttgaa aattcccgcg
     3661 acgagttttg ccatttcctg gccggtgatc ggctcgatgt ccaggttggc tttcttggcg
     3721 tcggcaagaa actctccatc cttcatcgtc gcggcgaatg cttggcgcag cgcgtcgacc
     3781 cgttgtttgg gggtgcgcgg cggggcgacg tagggacggt agaagtcggc gggatcttgg
     3841 acgccggcct gaagcagttg gcgcgcttca tccgtcttgg ccaggctttg cgccagcggg
     3901 acgttgggca ggtccggatg cggtttggcg acggcctgga gcacgacgat ggcctcaccg
     3961 gattgaatcg ctttactcca cgtcgagcga atcgagtccc aagtccagca gccgccggcc
     4021 aactcgccgc tttccgccgc caggcgaatc tccgcggtgc ccttgtagcc ggtgaccaa
//
LOCUS       DASQKS010000005         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5881893, whole genome shotgun sequence.
ACCESSION   DASQKS010000005 DASQKS010000000
VERSION     DASQKS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5881893"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VE170_00155"
     CDS             <1..1036
                     /locus_tag="VE170_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ13869.1"
                     /translation="KDMKRLLLLAVLLLVPGAEASAQTPYYQGKTINFIVGSGTGTAY
                     DIYSRLLANHIGKHIPGNPNVIMQNMPAAGGIVAANYVYSVAKPDGLTIASINPAHYF
                     NQLQGNKEIKFDWTKFTWLASPDKSEHLLYMRSDAPYKSMQDIRNAKEPPKCGATGTG
                     TSGHYIPRLLEELLGTKFTIVTGYAGGNEIDIATERNEVVCRSFTTQAYFSREPYHTW
                     RKTNLVRILMQTGEKKDPQLPDTPLLKDLMNEYKTPELSRRVAVVMLGSGELGRPMVT
                     HPATPPDRTKLLRDGFAKAMLDPALLDEAKQKKLDITPVSGEQLAKIAKEVIEQPAEV
                     VERIKKILAQ"
     gene            1090..1764
                     /locus_tag="VE170_00160"
     CDS             1090..1764
                     /locus_tag="VE170_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13870.1"
                     /translation="MKRAISTYGIISSGLILGLLVSTTAPVTAAANPWDDATNVETKE
                     RFIPVELWTGAEWDGKQELKMPKVDGNYRHNKAAYSIKGPTDWKHPATGQNHAVYERI
                     NPQRGGAKWQLFVINEERTGLGRLYDGRPNQVTRTSSGGLKFPLGLWKEGETKKHGYK
                     IYEGSRDSERVESVTIKKIDFILGNEPHCLEFYWSVTDRSEKKRYDHHTYTYCPGKSM
                     VHEIQH"
     gene            1792..2769
                     /locus_tag="VE170_00165"
     CDS             1792..2769
                     /locus_tag="VE170_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13871.1"
                     /translation="MCDFLDSDKLRVVTLLLKRSVVSGLLDLRQAMAVLEQTFREQAA
                     GQVQQVAPLRFMNRGMRMVVGGLQAQDRNGLRVSVTGGGALALLFEISSGKLLSLMGY
                     PFSELRISATVGLAIERFTDAKAKTVAMIGSGRLALPILEPACALRPIERVLVYSRSA
                     ENREGFARTASALLKTSVIAVASPQAAIAQADFVLVSTNSPAPALHGQWLRPGLAVFG
                     IGRPNEFDDDVYLMADLVSVPSKTHELNYYDTKLDQPLIRLSQQGKLSWDDVAEFCDV
                     VTDKVAVPDLARSLRIFRDSQGGYGDLTLAVYAFEEAKRRGLGEEISTE"
BASE COUNT          686 a          784 c          887 g          607 t
ORIGIN      
        1 taaggatatg aaacgactgc ttttgttggc ggtattgctg ttagtcccgg gcgcggaggc
       61 gagcgcgcaa acaccctatt atcagggcaa gactatcaat tttattgtcg gatcggggac
      121 cggcacggcc tacgatattt attcgcgcct attggcgaac catattggca aacatattcc
      181 tggcaatccg aatgtgatca tgcaaaacat gccggcggcc ggcggcatcg tcgcggccaa
      241 ttatgtttac agcgtcgcca agccggacgg cttgaccatc gcgtcgatca atccggcgca
      301 ctatttcaat cagctccagg gtaacaaaga aatcaaattc gactggacga agttcacctg
      361 gctcgccagt ccggataagt cggagcatct gctctatatg cgcagcgatg cgccgtataa
      421 gagcatgcag gatattcgca acgccaagga gccgcccaag tgcggcgcca ccggcaccgg
      481 caccagcggc cattacattc cgcgcctgct cgaagagctg ctcgggacca agttcaccat
      541 cgtcaccggc tatgccggcg gcaatgaaat cgacatcgcc acggagcgca acgaagtcgt
      601 ctgccggtca ttcaccaccc aggcatattt ttcccgcgag ccctatcaca cctggcgcaa
      661 aaccaacctg gtgcggatac tcatgcaaac cggcgagaag aaagacccgc agttgcccga
      721 tacgccgctg ctcaaagacc tcatgaacga atacaaaacc ccggagttga gccgccgtgt
      781 cgccgtcgtc atgctcggct ccggtgagct tggccggccg atggtgaccc atccggcgac
      841 gccgccggat agaacgaagc tattgcgtga cggatttgca aaagcaatgc tcgatccagc
      901 gctgctcgat gaggcgaagc aaaagaaact tgatattaca ccggttagcg gcgagcaact
      961 cgccaagatc gcgaaagaag tcatcgaaca gccggcagaa gtggtcgagc ggatcaaaaa
     1021 gatattggcg caatgaaatt atttaagaca acttcaaatg caacagcgta accattgtgg
     1081 cgatgcggta tgaaacgcgc gattagcacg tacgggatta tttcgagtgg tttgatcctg
     1141 ggcctgttgg tttcgactac cgcgcccgtc acggcggcag cgaatccatg ggacgacgcg
     1201 acgaatgtgg aaacaaaaga acggttcatt ccggtggagc tttggaccgg cgccgagtgg
     1261 gatggcaagc aggaacttaa aatgccgaag gtcgacggta actatcggca caataaagcc
     1321 gcttactcga tcaaagggcc taccgactgg aaacatccag cgaccgggca gaaccatgcc
     1381 gtctatgagc gaattaatcc tcagaggggc ggtgcaaaat ggcaactgtt tgtcattaac
     1441 gaagaacgca ccggtctcgg ccggctttac gatggacggc ccaatcaagt cactcggaca
     1501 tcgtcgggcg gtcttaaatt tcccctcggc ttgtggaaag aaggcgaaac caaaaagcat
     1561 ggctataaga tttatgaggg atcgagggat tctgaaaggg tggaatcggt cacgatcaag
     1621 aagatcgact tcatattggg taatgagccc cattgtctgg aattctactg gagcgtgacc
     1681 gacaggtccg agaaaaagcg ctacgaccat cacacctata cgtattgccc tggaaaaagc
     1741 atggttcatg agattcagca ctgacggcgc agggcgccgt ttgtcgatag gttgtgtgat
     1801 ttcctcgaca gcgataagtt gcgcgtcgtg acgctgctgc taaaacgatc tgtagtttcg
     1861 ggtttgttgg acctgcgcca agcgatggcg gtgctcgaac aaacatttcg cgaacaagcg
     1921 gccggtcagg tgcagcaagt ggcgccgctg cggtttatga atcgcggcat gcgtatggtc
     1981 gtcggcggct tgcaggcgca ggatagaaac ggtttgcggg tcagcgttac cggcggcggc
     2041 gcgttggctt tgttgtttga gatcagctcg ggcaagctgc tgtcgttaat gggctacccg
     2101 tttagcgagt tgcgtatcag cgccactgtc ggcctggcca tcgagcgctt caccgatgcc
     2161 aaagcgaaaa ccgtggcaat gatcggcagc ggccggctgg cgttgccgat tctcgagccg
     2221 gcctgcgcgt tgcgtcccat cgagcgcgtg ttggtttaca gccgcagcgc ggagaatcgc
     2281 gagggcttcg cgcgtacggc cagtgcgctg ctaaaaactt cggtgatcgc agttgcctcg
     2341 ccgcaagcgg ccatcgcgca agccgatttt gtcctagtga gcactaactc gccggcgccg
     2401 gccctgcatg gccaatggct tcggcctggg ctagcggtgt tcggcatcgg ccggcccaat
     2461 gagtttgacg atgacgttta tctcatggct gacctggtga gcgtccccag caaaactcat
     2521 gaactcaact attacgacac caagctcgat cagccactga ttcgcttgtc gcagcagggc
     2581 aagctttcct gggacgacgt cgcggaattt tgcgatgtcg tgaccgataa agtggcggtg
     2641 ccggatttgg cgcgcagctt gagaatcttt cgcgactccc agggcggtta tggcgatcta
     2701 actttggcgg tgtacgcctt cgaggaggct aagcggcgcg gcttgggcga ggagatcagc
     2761 actgagtagc gagcaagaag tcaggagtaa gcagatgaag attcggatat ggatcttgct
     2821 gtcggttgcg ctggtcagtc tattattcgg ccaggccgct aaggcggcgc aggcgcgctg
     2881 ggacgatgtc gtgcgggcgg cggaaaagga aggcgatgtc agcgtctatg cgaccaactc
     2941 cgtcggcgac cttcaggtga tttg
//
LOCUS       DASQKS010000006         5720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_842552, whole genome shotgun sequence.
ACCESSION   DASQKS010000006 DASQKS010000000
VERSION     DASQKS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842552"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4280)
                     /gene="gltB"
                     /locus_tag="VE170_00170"
     CDS             complement(<1..4280)
                     /gene="gltB"
                     /locus_tag="VE170_00170"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HYJ13872.1"
                     /translation="MTAAGLPEKQGLYDPRFEHDACGVGFVVNIKGEKSHEIVEQALT
                     VLENLDHRGACGCEENTGDGAGILMQVPHVFLKEACSGLGFQLPEPGDYGVGMIFFPD
                     HRQQRARFEKIIEAIVKEAGQHFLGWRKVPTDNLYLGDGAKAAEPFVRQMFIGRSADL
                     ADDLAFERKLFVIRRRAENAIRYAGLPGGEFFYVPSLSSRTLIYKGMLTSRQVSTFYP
                     DLTDPQLESAIALVHSRFSTNTFPSWGRAHPYRYLIHNGEINTLRGNENWMHARQGML
                     ASEMFGDDLTKVFPIIQQDGSDSTKFDNCLEFLALSGRSLPHAMMMMIPEPWENHESM
                     DAKKRAFYDFHSCLMEPWDGPASIAFTDGTVVGAVLDRNGLRPSRYYVTKDDLVIMAS
                     EVGVLDVPPERVLEKRRLQPGRMFLVDTSEGRIISDEEIKQEMAAAQPYGQWLKDNLV
                     RFNELSEVKDQEPPLDHAATLQRLQAFGYNFEDLRVNIGPMAQNSIQPVGSMGTDTPL
                     AVLSDKPQLLYNYFKELFAQVTNPPIDPIREELITSTTLTIGSEGNLVNPGPQSCRQL
                     RLAEPILKNADMEKLRQLSMPGIKSVTLPILFNVGEGKAGLERALDELFKAADAAIDD
                     GATIVILSDKGVDRTHAPIPALLATAGLHHHLIRAGTRTRVGLVLESGEPREVHHFCL
                     LIGYGAQAINPYLVYECLSDMIHQGLLPNISYHDAVKGYDKACYKGVVKVMAKMGIST
                     IKSYCGAQIFEAVGLGEELVNKYFTWTPSRIGGIGLEEIAREAQQQHGRAYPTYPLNG
                     HTLEVYGQYQYRKDGELHLFNPRTIHLLQKAARSNDYRTFKEYTRLIDDQSERIATLR
                     GLMVLKYGDKPIPIEEVEPVDAIVKRFKTGAMSYGSISKEAHEALAIAMNRIGGKSNT
                     GEGGEDPARYVLETNGDSKNSAIKQVASGRFGVTSYYLTQARELQIKMAQGAKPGEGG
                     ELPGRKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANHFARISVKL
                     VSEVGVGTIAAGVAKGHADVVLISGHDGGTGASPQTSIKHAGLPWELGLAETHQTLVL
                     NNLRSRIAVETDGQLKTGRDVVIAALLGAEEFGFATTALVTLGCIMMRVCHLDTCPVG
                     VATQNPELRAKFAGDPEYVVNFMRFIAQEMREHMAELGFRTINEMVGRADRIEMRRAV
                     AHLKAQGMDYSAILYQPKAGPEVGRYCQIPQNHGLENALDNQVLLDLAAPALERGEKV
                     KAKLDIRNTNRVVGTILGSEVTRRYGPQGLPEDTIHFHFQGSAGQSFGAFIPPGVTLE
                     LEGDANDYFGKGLSGGKVVLYPPEGSTFVPEENIIVGNVAFYGATNGEAYIRGMAGER
                     FCVRNSGVRAVVEGVGDHACEYMTGGRLVVIGKTGRNFAAGMSGGIAYV"
     gene            complement(4341..4838)
                     /locus_tag="VE170_00175"
     CDS             complement(4341..4838)
                     /locus_tag="VE170_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYJ13873.1"
                     /translation="MIDVIDLKILYILQNSGRKRLADIADEVELSAPAVMERVKKLEA
                     SGVIRGYQALIDSKKVGKDITAFIGVSIGNQSGIDLFAQQMLRYGDVLECHHVTGDES
                     FILKVKSANTASLEKLLGEIRSVDGVTRTVTNVVLSTAKESQALDLSAGLVENSSAKK
                     RNSAQ"
BASE COUNT         1202 a         1679 c         1563 g         1276 t
ORIGIN      
        1 acataggcta tgccgccgga catgccggcg gcaaagttgc ggccggtctt accaatcacg
       61 acaagccgcc ctcctgtcat atactcgcaa gcatggtcgc cgaccccttc gaccaccgcg
      121 cgcacgccgc tattgcgtac gcagaaacgc tccccggcca tgccgcgaat gtaggcctcg
      181 ccattggtcg cgccgtagaa ggcgacgttg ccgacgatga tgttttcctc gggcacgaag
      241 gtcgaacctt ccggcggata aagcacgacc ttgccgccgg acaagccttt gccgaaataa
      301 tcgttggcgt ccccttccaa ctcaagggtg acgcccggcg ggataaacgc gccaaagctc
      361 tgccctgccg atccttgaaa atgaaaatga atggtgtcct caggcaaacc ctggggaccg
      421 taacggcgcg ttacttcgct gccgagaatc gtcccgacga cccggttggt gttgcgaata
      481 tcgagcttgg ccttgacttt ttcgccgcgc tccaatgccg gcgccgcaag atcgagcagc
      541 acttgattgt ccagcgcgtt ttccaagccg tggttctgcg ggatctgaca gtagcggccg
      601 acctcgggac ctgccttcgg ctgataaagt atcgccgagt agtccatgcc ttgcgctttc
      661 aaatgggcga cggcacggcg catctcgatg cgatcggcgc ggccgaccat ttcattgatc
      721 gtgcgaaagc ccagctccgc catatgctcg cgcatttctt gagcgatgaa gcgcatgaag
      781 ttgacgacat actcgggatc gccggcgaac ttggcgcgca gctcgggatt ttgcgtcgcc
      841 acaccgaccg gacaagtatc gagatggcac acccgcatca tgatgcaacc cagtgtcacc
      901 agcgccgtgg tggcaaaacc aaactcctcg gcgccgagca gtgcggcgat cacgacatca
      961 cggccggttt tcaattggcc gtcggtttcc acggcaatcc gactgcgcag gttattaagc
     1021 accaaggtct ggtgggtttc cgctaaaccg agttcccatg gcaaacctgc atgtttgatg
     1081 ctcgtctgtg gcgaagcccc agtgccgccg tcatggccgc tgatcaagac cacatcggca
     1141 tgccctttag cgacaccggc ggcgatcgtg cctacgccga cttccgaaac cagcttgacg
     1201 ctgatccggg caaaatgatt ggcgttcttg agatcgtgaa tcagctgcgc caaatcttcg
     1261 atggaataaa tatcatggtg cggcggcggc gagatcagac ccacgcccgg cgtcgaatgg
     1321 cgcaccttgg cgatccatgg ataaactttt ctccccggta attcaccgcc ttctcccggc
     1381 ttggcgcctt gggccatctt gatctgcaac tctctggctt gggtaagata gtaactggtg
     1441 acaccgaagc ggccggaagc cacttgcttg atcgcgctat ttttcgagtc gccgttggtc
     1501 tcgagcacgt agcgcgccgg atcctcgccg ccctcgccgg tattgctttt gccgccgatg
     1561 cggttcattg cgatcgccaa ggcttcatgg gcttctttgc taatcgagcc ataggacatg
     1621 gcgccggtct taaatcgttt gacgatcgcg tccaccggct cgacctcctc gatggggatc
     1681 ggtttgtcgc cgtacttgag caccattaga ccgcgcaagg tcgcgatccg ctccgactga
     1741 tcatcgatca gccgggtgta ttctttgaac gtccggtaat cgttgctgcg cgcggctttt
     1801 tgcaacagat gaatcgtccg cgggttaaac agatggagct cgccatcctt gcggtattgg
     1861 tactggccgt agacttccaa cgtgtggccg ttcagcggat aagtcggata cgcccgccca
     1921 tgctgttgct gcgcttcacg cgcgatttct tcgagaccga tgccgccgat tcgcgacggg
     1981 gtccaggtga aatacttatt caccagttct tcgcccaacc ccaccgcctc gaaaatctgc
     2041 gcgccgcagt aggacttgat ggtcgagatg cccatcttag ccatcacttt aaccacgccc
     2101 ttgtagcacg ctttatcgta acctttgacg gcatcatggt agctgatgtt tggcagcagc
     2161 ccttgatgaa tcatgtcgct caagcattcg taaaccagat agggattgat cgcttgagca
     2221 ccgtaaccga tcagtaaaca gaaatggtgc acttcgcgcg gctcgccgga ttccagcacc
     2281 aagccgacgc gggtgcgcgt gccggcgcgg atcagatgat gatgcaagcc ggccgtggca
     2341 agcagcgcag ggatcggcgc atgggtgcga tcgacgcctt tgtccgacaa aataacaatt
     2401 gtcgcaccgt cgtcgatcgc cgcgtctgcg gctttgaata actcatcgag cgcgcgctcc
     2461 aagcccgcct tgccctcgcc gacattgaat aagatcggca gggtaacgga ttttatcccc
     2521 ggcatgctaa gctgccggag tttttccata tcggcgtttt ttaaaatcgg ttcggccaag
     2581 cgcagctgcc ggcaactctg cggccccggg ttgacgagat tgccctcgct gccgatggtc
     2641 aatgtcgtcg aggtgatcaa ttcttcccgg atcggatcga tgggcggatt ggttacctgg
     2701 gcgaacagct ctttgaaata gttataaagc aactgcggct tatcggacaa aaccgccagc
     2761 ggcgtatcgg tgcccatgga gcctaccggc tggatgctgt tttgcgccat cggaccgata
     2821 ttgacgcgca gatcttcgaa gttgtaaccg aaggcttgca aacgctgcag cgtcgctgcg
     2881 tgatccagtg gcggttcttg atctttcact tccgacaact cgttgaaacg caccaaattg
     2941 tctttcagcc attggccata gggctgtgcc gctgccatct cctgcttgat ctcttcgtcg
     3001 ctgatgatac ggccctcgct ggtgtcgacc aaaaacatcc ggccgggttg cagccggcgt
     3061 ttttccaaca cccgctccgg cggcacgtca agcacgccca cctccgacgc catgatgacc
     3121 agatcgtctt tggtcacgta gtagcgagac ggccgcagac cgttgcgatc gagcacggca
     3181 ccgaccacgg tgccgtcggt aaaggcgatc gacgccgggc cgtcccaggg ttccatcaga
     3241 cagctatgga aatcgtagaa ggcgcgcttt ttggcatcca tgctctcgtg attctcccag
     3301 ggctctggga tcatcatcat catggcatgg ggcaatgagc gaccgctcaa tgcgagaaac
     3361 tccagacagt tgtcgaattt ggtcgaatcg ctgccatctt gctgaatgat cggaaagact
     3421 ttcgtgagat cgtcaccgaa catttccgag gccaacatcc cctggcgcgc atgcatccag
     3481 ttctcattgc cgcgcagggt attgatctcg ccgttgtgaa tcagataacg atagggatgc
     3541 gcccgccccc aactcggaaa cgtgttggtg ctgaagcgcg aatgcaccag ggcgatggcc
     3601 gactcgagct gtgggtcggt taggtcagga taaaacgtcg acacttggcg cgacgtcaac
     3661 atgcccttat aaatcaacgt ccggctcgac aggctcggca cgtagaaaaa ttcgccgccc
     3721 ggcagtccag cgtaacgaat cgcattctcg gcgcgacggc ggatcacgaa caatttgcgc
     3781 tcgaacgcca ggtcgtccgc aagatcggcg ctgcgaccga taaacatttg gcgcacgaac
     3841 ggttcggccg ccttggcgcc atcacccagg taaagattgt ccgtcggcac cttgcgccag
     3901 ccgagaaaat gctggcctgc ttctttcacg atcgcctcga tgattttttc gaagcgcgcg
     3961 cgttgctgac ggtgatccgg gaagaagatc atgccaacgc cgtagtcgcc cggttccggc
     4021 aactgaaacc ccaaaccgga gcaggcctct ttaagaaaaa cgtgcggcac ttgcatcagg
     4081 atgccggcgc cgtcaccggt gttttcttcg cagccgcagg cgccgcggtg atcgaggttc
     4141 tctaataccg tgagcgcttg ctcgacgatc tcgtgggatt tttcgccttt gatattaacc
     4201 acgaaaccaa cgccgcaggc atcatgctca aagcgcggat cgtagagacc ttgtttttca
     4261 ggcaatccag cagcagtcat gtgattgatc ctttaccttc gctatcctgc tatacttttt
     4321 aggctgttat catcgagcgc tcactgagcg ctatttcttt ttttcgcact ggagttttcg
     4381 accaggcccg cgctcaagtc gagcgcttgg ctctccttgg ctgtcgatag gacgacgttg
     4441 gtcacggtgc gcgtcacccc gtccacagag cgaatctcac ccaatagttt ttccagcgaa
     4501 gcggtattgg cggacttcac tttgagaata aaactctcgt caccggtcac gtgatgacac
     4561 tccaaaacat cgccataacg aagcatctgc tgggcgaaga gatcgatgcc gctttggttg
     4621 ccgatcgaca caccgatgaa ggcggtaata tccttaccga cctttttgct atcgatcagc
     4681 gcctggtaac ccctaataac cccgctcgct tcgagcttct tcacccgctc cataaccgcc
     4741 ggcgccgaca actccacctc gtcggcgata tcggccagac gcttacgccc gctattttgc
     4801 agaatataaa ggatttttaa atctataacg tcaatcaagt taaaattatt aagccttttc
     4861 ccaaattagc ttaaactttt tatgccatcg tcaaaaaata ctgtcaagtg aattgcacag
     4921 aaaacttaca attttgcctg gaatggggtc tgacttgggg tcaaaaccac cgtcccacag
     4981 gaacacagga ctagccaaag gaacgacgag cggcgtctag tcggagacta atctgccggt
     5041 ggactcgatt gtaattgccc cggttgccgc gcacacggga atgccgcgcg agaaactaat
     5101 ctggaaacta gccccgacgc cgttggagtt agccggcaat ggcgcatcgc cgtgaatgat
     5161 cagcatcacg gtgcgtgaac gcgagagatt ttaggtactc gataggtcat ttggccaggc
     5221 tcggcgagcg aaaggaccca acaacctgca aggcctgcga gctgagccac agatcggtat
     5281 tgtcgggatc gatgagcaga tgcttcagga taggtatcgg tcttcttcat cgtcgagaaa
     5341 cgttcgctgc cgtcgattgc tacgggtcaa tatatggtgg aacgggcgcg ctcatactcg
     5401 agccgggggt tggtgttatg gaacgaaagc tgtcggaaag aagaaatcga tccgcggcct
     5461 gtgtcgccat ggcgggagat aagccgagtt gcgctcgcgg cgacgccgcg ctgtaacctt
     5521 cgtcatgaaa attcttatac cgtggcgccg atcatgttgt atctgtcggt cgttggtcta
     5581 tggcttcgac gatcgcttga tagatatcct ccgccgcccg ctccttgttc aggcagacat
     5641 acgcgccggc atctttcatc gccccttgga ccgacgagtc gctggcgaac gataacccga
     5701 tgacgatcat gcccggctgc
//
LOCUS       DASQKS010000007         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4202734, whole genome shotgun sequence.
ACCESSION   DASQKS010000007 DASQKS010000000
VERSION     DASQKS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4202734"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1555
                     /locus_tag="VE170_00180"
     CDS             74..1555
                     /locus_tag="VE170_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HYJ13874.1"
                     /translation="MNPAHPRGKVYFVDTTLRDGQLSLWASNMRTGMMLPIAERLDRA
                     GFEAIEIMSSAFYKKCVRDLQDDPWERIRLLAQRIRKTPLRSIRSRSMLAFQLTAPAI
                     TDLWLERLAANGIEELRTSDPSNTPRYWREAVDAAKRVGLKTILNIIYSISPKHTDEY
                     FVQRTREAAKLDVERICFKDPGGLLTPEATRRLVPLIQREANGKPVEFHTHCNTGLGA
                     LCCLEAIQLGITSINTAIPPLADGSSNPSLFNVAMNARALGYQTMVDEQVVKPVRDHF
                     MMVAKRENLPIGRPLAYDALHPLHQVPGGMISNFRFQLGNLAKLDQLPAVLEEVARVR
                     AEFGYPIMVTPYSQFFGVQAAINVMVGARYQEVTDEVLLYALGYWGEEEAQSIDANLR
                     DRLVGSARARELAAIKVPDIGLAEFRMKFAGNSDDDKLLNFFAGESALAALKAAGAPQ
                     DYASTATPLVALIEQLTRRQDSKQVYIKREGFSLRMARRGSIH"
     gene            1635..2309
                     /locus_tag="VE170_00185"
     CDS             1635..2309
                     /locus_tag="VE170_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HYJ13875.1"
                     /translation="MLWLRQLLNLVTLGVAAMLLCFADGAAYSQAITSWACTESESTC
                     KSVSIVHNSWHAAIVLRNSDLAIRNMPELADFPSAQFIEFSWGDKDYFPDPQSGIFGA
                     IKAALWSGGSVIHLVGFTDTLEHFYRGATITELRLNPAAYERMVDYIDATFRREPSSG
                     RAHAAPGLFSFSRFYPASRSFSLLRTCNTWIAEALEHAGLSISPTFVVTAGNLQSQLS
                     AAKPMP"
     gene            2500..>2938
                     /locus_tag="VE170_00190"
     CDS             2500..>2938
                     /locus_tag="VE170_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13876.1"
                     /translation="MKYRHIVPAGIAIALLASACQTPAPLPMPQPPPELAQEAHKPPA
                     PAAPVATATPQEKPEEVVGKARIDRSLPESLIAAPDKDRAPRNIDYGTMLSSRAANTR
                     FTNSWQYRRTRQGQIVGFEFSNHGGNSILTPRRDATKNQFFTRD"
BASE COUNT          602 a          945 c          807 g          584 t
ORIGIN      
        1 gtaatggaat gttgcttact ccgaacccca ttactccaac actccactac tccatcactc
       61 caatcccact gccatgaacc cagcgcatcc ccgcggtaaa gtctatttcg tcgacaccac
      121 cctgcgcgac ggccagttga gcctttgggc ttccaacatg cgcaccggta tgatgttacc
      181 catcgccgag cggctcgacc gcgccggctt cgaagccatc gagatcatgt cgtcggcgtt
      241 ctacaaaaaa tgcgtgcgcg atttgcaaga cgacccgtgg gagcgcatcc ggctgttggc
      301 ccagcgcatc agaaaaaccc cgctgcgctc aattcgcagc cgctctatgc tggcgtttca
      361 gttgaccgcg ccggcgatca ccgatctctg gttggaacga ttggccgcca acggtattga
      421 ggaactgcgc acctccgatc cgtcgaacac accgagatat tggcgcgaag cggtcgacgc
      481 cgccaagcgc gtcggcctca agacgatctt gaatattatt tattcgatct cgcccaagca
      541 caccgacgaa tattttgtcc aacgtacccg cgaagcggcc aagctcgacg tcgaacggat
      601 ctgcttcaaa gatcccggcg gcctgctgac cccggaagcg acccgccgtc tggtgcctct
      661 gatccagcgc gaagcgaacg gcaaaccggt ggaatttcac acccactgca acaccggcct
      721 cggcgcgctt tgttgcctcg aagcgatcca gctcggcatc acgtcgatca acacggcgat
      781 cccgccgctc gccgacgggt cctcgaatcc atcgctcttc aacgtcgcca tgaatgcccg
      841 tgcgctgggc tatcagacca tggtcgatga gcaggttgtc aaaccggtac gcgaccattt
      901 catgatggta gcgaagcggg agaatctgcc gatagggcgg ccgttggcat acgacgcgct
      961 gcacccattg caccaggtgc ccggcggcat gatttccaat tttcgttttc agctgggcaa
     1021 tttggccaag ctcgaccagc tgccggcggt gctcgaagaa gtcgcacgcg tccgtgccga
     1081 gttcggctat ccgatcatgg tgacgcccta ttcgcagttc ttcggcgtcc aagccgccat
     1141 caacgtcatg gtcggtgcgc gctaccagga agtcaccgac gaagtgctgc tttacgccct
     1201 gggctattgg ggcgaagagg aagcgcagtc catcgacgcc aatttgcgcg atcgcttggt
     1261 cggcagtgcg cgcgccagag aattggccgc aatcaaagtc cccgacatcg gcttggcaga
     1321 attccgaatg aaatttgccg gcaacagcga cgacgacaag ctactaaact tctttgccgg
     1381 tgagtctgcg ctggcggcgc taaaagccgc cggcgcgcct caagattatg ccagcacggc
     1441 aacaccgctg gtcgcactga tcgagcagtt gacccggcgc caggactcaa agcaggtcta
     1501 tatcaagcgt gagggcttta gtttgcgcat ggcgcgacgg ggttccattc attaagttac
     1561 ggcgcgcgtg ttacgcgctc gcagccgcgc ctatgcggtt gcttaagccg gcaatccgcg
     1621 gctagcatga ggccatgctc tggttgcggc aactattgaa cttggtgacc ttgggcgtag
     1681 cggcaatgct gctctgcttc gctgatggag ccgcctacag ccaggccatt acttcttggg
     1741 cttgcaccga aagtgaatcg acttgtaaat ccgtgtcgat cgttcacaac tcctggcacg
     1801 ccgccatcgt cctgcgcaac agcgacctcg caataagaaa catgcccgag ctcgcggatt
     1861 ttccctcggc gcaatttatc gaattcagct ggggcgacaa agattatttc cccgatcccc
     1921 agtcgggaat cttcggcgcc atcaaggcgg ccctgtggtc cggtggcagc gtcattcacc
     1981 tggtcggatt tacggacaca cttgaacatt tctaccgcgg cgcgaccatc accgaactgc
     2041 gcttgaaccc ggccgcctat gaacgcatgg tcgattatat cgacgcaact ttccgccgcg
     2101 aaccatcgtc cggccgtgcc cacgccgctc ctgggctgtt ctccttcagc cgcttttatc
     2161 cggcaagccg cagttttagc ctgctgcgca cctgcaatac ttggatcgct gaggcgctcg
     2221 aacacgcggg gctgtcgatc tcgccaactt ttgtcgtcac cgcgggtaat ttgcaaagcc
     2281 agctgtcagc ggccaagccg atgccttgag cgccgcggtg tgtcttcagc atgtcaggac
     2341 aagataaata ttgcccatgc gacggccaac aggcacaccg gcgccgacat cgacctccgt
     2401 tgtccccgcg gcggcgaccc ctcaactgct gggcatttgt cgcacagccg gcgcaagcat
     2461 cgcagcctcg ggtagatagt gctaaactgg cggcttgcta tgaagtatcg ccatattgta
     2521 ccagccggca tcgccatagc gctgctcgcc agcgcgtgcc aaacaccggc accgctaccg
     2581 atgccgcagc cgccgccgga attggctcaa gaagctcaca agccgccggc gcccgctgcg
     2641 ccggtggcaa ccgcgacacc gcaggaaaaa cctgaagagg ttgtcggcaa agcgcggatc
     2701 gatcgcagtt tgccggagtc gctcatcgcc gctccggaca aggaccgcgc gccgcgtaac
     2761 attgactacg gcacgatgct gtcgtcccgc gcggcgaaca cgagattcac taacagctgg
     2821 cagtatcggc gcacgcgtca aggccaaatt gtcggcttcg aattttccaa tcatggcggc
     2881 aactcaattc tcacgccgcg tcgcgatgcg accaagaatc aatttttcac ccgcgatt
//
LOCUS       DASQKS010000008         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5882502, whole genome shotgun sequence.
ACCESSION   DASQKS010000008 DASQKS010000000
VERSION     DASQKS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5882502"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /locus_tag="VE170_00195"
     CDS             <1..2477
                     /locus_tag="VE170_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13877.1"
                     /translation="LVAALSATGIPAVGHGQSRELALEKILNPMPDFDPFETPASAPQ
                     FFPDAVDKRARELLIDTLTYRDQALNDHLMFLREEDARLQREQRTSTGLTERAQDLVN
                     NTIQDREKNLAAYREALRKASSPERKKYLESVLNRDDHFQSEQLMRQGSTNFWGGLVN
                     RMLSSIDLVGVASGNYIGAAAETAISQVYALMDRDMPMEERRALARDLDHLKRYPDDP
                     KNAQVRKRVEALDKKKRHLLAAKQLAKAKEALSKGDDSRALFHTQVAAFFDPQSQDAA
                     KLSQQTAQLLSARQTARQQSLKARKDENLSAEQQADAEKLLTALSLRDANLIQRVAID
                     TEKKHPDKALADAARDAEAVALEMKGWHEAARKAVEQNARKAATPVAKERANVLLQSP
                     GYNRLATFQDARTERQLESVKYVLLGEDFLRKNMLFAAGAMAAAGPGGAATLGMANAL
                     MWSRNLFQVMTNNPISAQPVIDAGVAYVRNHPKSENASDVYRVLADAYEERGLIAPAL
                     TYHELAGSPKEKLDALNDKAARGLLDAASKSSSRGRQEDFLKAVLDYHPHSSAAAEAT
                     KKLAVLAKEENQGMRMSKQFLLEHREIWGPRGLALKATLFDGNLRNMEIAERGAILLN
                     DNELMVFYQTPWGVRGQNHRLTKAATERFYVALREKNQQVAAADANQRGKDSVGGIRN
                     VPEAIVRGERDRRGQSSEDREDTTFTLIRDAGGSSYRRVLDAELITENERNPGSQYQL
                     PPIQGSISASRFNMTGALPTGFGGSQLAIGADHRAPYGGVQFPIPLLQGFIPVDFMVQ
                     GRPGGVSIYPRIRTGVDSGSDPELYR"
     gene            2632..2787
                     /locus_tag="VE170_00200"
     CDS             2632..2787
                     /locus_tag="VE170_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13878.1"
                     /translation="MAIFAGYLMALDPWCDEIKESHYKSKKISAKSDFVDSSMIEEGQ
                     KLAGFKP"
     gene            2826..3833
                     /locus_tag="VE170_00205"
     CDS             2826..3833
                     /locus_tag="VE170_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13879.1"
                     /translation="MKIVTRLQLICLFLTIALAGEVAAQTPPLKKIRIGYPSLSFRQS
                     NVWVAREVGLFTKYGLEVEPIFLRGGQMATQALVAGDPPIVNIGTVVQASLQGYNLVL
                     IAAVETKYDQIVFARKGINKLEELKGKRFGISGYGSATHNAANILFQHLNLEVNKDIA
                     LVPAGPDAERMGAMMGGRIDATFFTSSAAAPARKNGFNELFSVADLGVEVQGNGFATS
                     RSYIASNRDTVKNALKGFVEAIYFVYANKKEAQRVFAKYMRTNDPEMLEDSYNGYIKW
                     IPKKPYPTLKGIQYMLDLVAPQLPQAKTAKPEQFVDLSFLQELEKEGFFNEMAKRYPS
                     K"
BASE COUNT          938 a         1204 c         1085 g          714 t
ORIGIN      
        1 ccttagttgc cgctttgagc gcaaccggga ttcccgccgt aggccacggc caatcacgcg
       61 agctggcgtt ggaaaaaatc ctcaacccga tgcctgactt tgacccgttt gaaacaccgg
      121 ccagcgcgcc ccagtttttc cccgacgcgg tcgacaaacg ggctcgggaa ttgctgatcg
      181 acacattgac ctatcgcgac caagccttga acgatcattt aatgtttttg cgcgaagagg
      241 atgcccggct gcaacgggaa cagcgcacca gcaccggtct gaccgagcgg gctcaagatc
      301 tggtcaacaa tacgattcag gaccgcgaga agaatctggc ggcgtaccgt gaggcgctgc
      361 gcaaggcgtc gtcaccggaa cgtaaaaaat atctcgagtc ggtgctcaac cgcgacgatc
      421 actttcagtc cgagcagctc atgcgccagg gctcgaccaa tttttggggc ggcttggtca
      481 atcgcatgtt gagctccatc gatctagtcg gcgtcgcatc gggcaattac atcggcgccg
      541 ccgccgaaac cgccatcagc caggtgtatg ccctaatgga tcgggacatg ccgatggaag
      601 agcggcgcgc cctggcccgc gacctcgatc acttgaagcg ctatcccgac gatcccaaga
      661 acgcccaggt gcgcaagcgc gtggaagccc tcgacaagaa aaaacgccat ctcctggccg
      721 ccaagcagct cgccaaagcc aaagaagcct tgagcaaggg cgacgacagc agggcgctgt
      781 ttcataccca agtggcagct ttcttcgatc ctcagtcgca ggatgccgcg aagttgagcc
      841 agcaaaccgc gcaactcttg agcgcacgcc aaaccgcccg ccagcaaagc ctcaaggcgc
      901 gcaaagacga aaacttgagc gcggagcagc aagccgacgc agaaaaactt ttgacagcat
      961 tatctctgcg cgatgccaac ttgatccaac gggtcgccat cgacacggag aaaaaacatc
     1021 ccgacaaggc gctcgccgac gccgcccgcg acgccgaagc cgtcgccttg gagatgaagg
     1081 gatggcatga agcggcgcgc aaggcggtcg aacaaaacgc ccgtaaggcg gcgactccgg
     1141 ttgccaaaga gcgcgccaac gtcctactgc aaagccccgg atacaatagg ctggcgacct
     1201 ttcaagatgc ccgcaccgag cgccagctgg agtcggtaaa atatgttctg cttggcgaag
     1261 acttcctgcg caagaatatg ctgttcgccg ccggcgccat ggccgccgcc ggtcccggcg
     1321 gcgccgcgac cttaggcatg gccaacgctt tgatgtggag cagaaatctt tttcaagtca
     1381 tgaccaacaa tccgatctcg gcgcagccgg tgatcgatgc cggggtcgct tatgtgcgca
     1441 atcatccgaa gtcagagaac gccagcgacg tttaccgggt gcttgccgac gcctacgaag
     1501 aacgcggcct gatcgcaccc gccctcacgt atcacgagct tgccggctca cccaaggaga
     1561 agctcgacgc cctcaatgac aaagccgcgc gcggcctact agacgccgcc agcaagtcga
     1621 gttcgcgcgg caggcaggaa gattttttaa aggcagtgct cgattatcat ccgcattcca
     1681 gcgccgccgc cgaagcaacc aaaaagctcg ccgtcctcgc caaagaagaa aaccagggca
     1741 tgcgcatgag caagcaattc ctactcgagc atcgggagat ctgggggccg agaggattgg
     1801 ccctcaaggc gaccctcttc gacggcaacc tgcgcaacat ggaaatcgcc gaacggggcg
     1861 cgatcttgct caacgacaac gagctgatgg ttttctacca gaccccctgg ggcgtgcgcg
     1921 gccaaaacca tcgtctgacc aaagcggcca ccgaacgttt ctacgtcgcc ctgcgggaaa
     1981 aaaaccagca agtcgccgcc gccgatgcca accagcgcgg caaagacagc gtcggcggta
     2041 tccgcaacgt acccgaggcg attgtccgcg gcgaacgcga ccgccgcggc cagagctccg
     2101 aagaccgcga agacacgacc ttcacgttga tccgcgacgc cggcggttcg tcctatcgcc
     2161 gggtgctcga cgccgaattg atcacggaga acgaacgcaa tcccggcagc caatatcaac
     2221 tgccgccgat ccagggcagc atctcggcga gccgcttcaa catgaccggc gctctgccca
     2281 ccggctttgg cggcagccag ctggcgatag gtgccgatca tcgcgcccct tatgggggtg
     2341 tacagtttcc cataccgctg ctgcaaggct tcattcccgt cgacttcatg gtccaaggcc
     2401 gccccggcgg cgtttcgatt tatccgcgca tccgtaccgg cgtcgatagc ggctcggacc
     2461 cggaacttta tcgttgatca cccatgcgcg aatttcgccg cccggtttgt attgcccggc
     2521 gaacgagtgt aagagtgcgc agatcgctct cggcgccgtc gttaaccgat ctccatcttg
     2581 catgaatcca tgaggttttt cccggttcga aaagaaaact tccgcggcgg catggccatt
     2641 ttcgctggtt acttgatggc cctggacccc tggtgcgacg agatcaagga aagccattac
     2701 aaaagcaaga aaatttccgc caagagcgac ttcgtcgact cctcgatgat cgaggaaggg
     2761 caaaagctcg ccggcttcaa accatagttg acaatcatca gaaaggactc gttaacgagc
     2821 taaccatgaa gatcgtaacc agactgcagc tcatttgcct atttttgacg atagcgttgg
     2881 cgggcgaggt agccgcacaa accccgccac tcaagaaaat tcgtatcggt tatccgtctt
     2941 tgagcttccg ccagagcaac gtttgggtcg ctcgggaagt cggcctgttc accaagtacg
     3001 gcctggaggt tgagccaatt tttttacgcg gcggccagat ggcgacccag gcgctggtgg
     3061 ccggggatcc gccgatcgtc aatatcggca ccgtcgtgca agccagcttg caaggctaca
     3121 acctggtgct gatcgccgcc gtcgaaacca agtacgatca gattgttttc gcgcgcaaag
     3181 gcatcaacaa actcgaagag ctcaaaggca agagattcgg catcagcggc tacggctcgg
     3241 caacccataa cgctgccaac atcctattcc agcacctcaa ccttgaggtc aataaagaca
     3301 tcgcgctggt tcccgcaggg ccggatgcgg aaagaatggg agcgatgatg ggaggacgaa
     3361 tcgatgcgac cttcttcacc tcttcagcgg cagcgccggc acgcaagaat ggcttcaacg
     3421 agctattttc ggttgctgat ttgggagtcg aagtccaagg caacggcttt gcaacgtcgc
     3481 gcagttatat tgcgagcaac cgcgacacag tgaaaaacgc gctcaagggt ttcgtcgaag
     3541 cgatttactt tgtctacgcc aacaagaagg aggcgcaacg ggttttcgcc aaatacatgc
     3601 gcaccaacga tccagagatg ctcgaagatt cttacaatgg ctacatcaaa tggattccca
     3661 agaaacccta tcccacgctc aagggcatcc aatacatgct cgacctggtc gcgccgcaac
     3721 taccccaggc taagacagcc aagcccgaac agttcgtcga tctgagcttc ttgcaggaac
     3781 tcgaaaagga gggcttcttc aatgaaatgg cgaaacgcta tccgagtaag taggatcatt
     3841 gaactaatga aaaattgcga ccacttcagt atttcaatac tgccatccgt gacggccacg
     3901 ctggtggctt gcttcgccgc cagtctgctt atcgtcggca c
//
LOCUS       DASQKS010000009         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3363178, whole genome shotgun sequence.
ACCESSION   DASQKS010000009 DASQKS010000000
VERSION     DASQKS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3363178"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /gene="guaA"
                     /locus_tag="VE170_00210"
     CDS             complement(<1..1531)
                     /gene="guaA"
                     /locus_tag="VE170_00210"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYJ13880.1"
                     /translation="MIVVLDFGSQYTQLIARRIRESKVYCEIHPFNVALEKITALDPK
                     GIILSGGPASVYQDDAPKVSAELLALPVPILGICYGMGVLNLHFGGAAARADRREYGP
                     AQLVIDNDSDLFHGFSRRDTTQVWMSHGDKMTSFPKGWEILAHSDNSPIASFADPTRR
                     FFGLQFHPEVAHSARGRQVLDNFVFRICGDRPDWTMEHFIESSVRKIRQQVGDGRVLC
                     ALSGGVDSSVAATLVHRAIGARLICVFVNNGLLRKNEAEQVCGLFAERFGASFRYADA
                     AESFLAELQGVEDPEVKRKKIGYKFIQVFEEEARKLGDIEFLAQGTLYPDVIESVSVL
                     GPSATIKSHHNVGGLPTDMKFKLIEPLRELFKDEVRVLGKELGLPPEMIQRQPFPGPG
                     LAIRVLGEVTEARLKVLRDADVIVLDEIKAAGLYDKVWQSFAVLLPVKTVGVVGDERS
                     YENVVALRVVDSQDGMTANWVALPFDLLGRISNRILNEVRGVNRVVYDISSKPPATIE
                     WE"
     gene            complement(1528..2991)
                     /gene="guaB"
                     /locus_tag="VE170_00215"
     CDS             complement(1528..2991)
                     /gene="guaB"
                     /locus_tag="VE170_00215"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYJ13881.1"
                     /translation="MLEQEVLPVGLTFDDVLLIPAESSVLPKDIDVSSRLTDRIRLSI
                     PLVSAAMDTVTESKTAIGMAQEGGLGIIHRNLSVWAQASEVEKVKKFESGMISDPITI
                     APEQKISEAQGIMTQYGISGLPVTKNGKLVGILTNRDLRFERRLDRKVSEVMTKDNLV
                     TVRPGVDLETAKELLHRHRIEKLLVVDDNFELKGLITVKDIQKTIQYPGACKDQRGRL
                     RVGAAVGVSDWEPRVEGLVRAGVDLIAVDTAHGHSKNVLDVVGQIRRRYPDLDIAAGN
                     VATGEGTSALIQAGVNVVKVGVGPGSICTTRIVSGVGVPQITAIGSCAKAAAKHNVAI
                     IADGGIKFSGDITKALAAGAHSVMIGSLFAGTEESPGETILYQGRTYKAYRGMGSIGA
                     MGQGSRDRYGQADVTDSNKLVPEGIEGRVPYKGSLSSNILQLVGGLRAGMGYLGCKEI
                     GALHSKAKFMQITAAALREGHVHDVFITKEAPNYRVE"
     gene            3162..3395
                     /locus_tag="VE170_00220"
     CDS             3162..3395
                     /locus_tag="VE170_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYJ13882.1"
                     /translation="MPLYEYECTQGHRFEALQKVSDKPLKKCDQCRAVAKRAVSQTIL
                     LHNHGVHVFDRVTGDDVLRGRPSSLKSFKKDKF"
     gene            3522..>4818
                     /locus_tag="VE170_00225"
     CDS             3522..>4818
                     /locus_tag="VE170_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HYJ13883.1"
                     /translation="MLAKVNSAALYGIDALRVEVEIDLASGLPQLATVGLPEGAVKES
                     KDRIRAAVKNCGYTFPAKRITINLAPADIKKEGSAYDLPMAIGILAAEGTLDKTLLDS
                     YFLIGELSLDGSVKPVRGALSIAIAAKKEKFRGLILPEQNASEAAVVSDLEVLGVRDL
                     PQVVEFLNGNRTIIPTGANLDDLFSQHSQYNADFNDVKGQQHVKRALEVAAAGGHNVI
                     LVGPPGSGKTMLAKRLPTILPDLTLAEALETTKIHSVMGTLGGQAIIATRPFRSPHHT
                     ISDAGLVGGGTVPRPGEVSLAHNGVLFLDELPEFRRNVLEVMRQPLEDGSITVSRAMG
                     SINYPASFMLVAAMNPCVCGFFTDPQKECICTPLQIQRYRSKVSGPLLDRIDIQIEVP
                     GLRYQELASKDSGESSTVIRERVNHARKIQLRRFEKTKIH"
BASE COUNT         1023 a         1502 c         1330 g          963 t
ORIGIN      
        1 attcccactc aatcgtggcc ggcggcttgg atgaaatatc gtagaccacg cggttgacgc
       61 cgcgcacttc gttgagaatg cggttggaga tccggccgag taaatcgaag ggcagcgcca
      121 cccagttggc ggtcatgccg tcctggctgt cgacgacccg cagcgccacg acgttttcat
      181 agctgcgctc atcgccgacc acaccgaccg tctttaccgg caatagcacg gcaaaagatt
      241 gccaaacttt gtcgtaaagc ccagcggctt tgatctcgtc gaggacgatg acatcggcgt
      301 cacggagcac tttcaaacgc gcttcggtca cctcgccgag cacgcgaatc gccagtccag
      361 gcccggggaa cggctggcgc tggatcatct cagggggcag gccgagctct ttgccgagca
      421 cgcgcacttc gtctttgaac agttcgcgca agggctcgat caatttgaac ttcatgtcgg
      481 tgggcaagcc gccgacatta tggtgcgact tgatggtcgc cgacgggccc agcaccgaca
      541 ccgattcgat gacatccgga tagagcgtgc cctgcgccaa aaactcaata tcgccgagct
      601 tgcgcgcttc ttcttcgaaa acttgaatga acttgtagcc gatcttcttg cgtttcactt
      661 cagggtcttc gacgccttgc aattccgcga gaaatgactc ggcggcatcg gcgtagcgaa
      721 agctggcgcc gaaccgttcg gcaaacaagc cgcacacctg ctcggcttcg ttcttacgca
      781 acagtccgtt gttgacgaac acgcagatca ggcgcgcgcc aatcgcacgg tgcaccaaag
      841 tcgccgccac cgaagagtcg acgccgccgc tcagtgcgca aagcacccgg ccatcgccca
      901 cttgctggcg aattttccgc accgagcttt caatgaaatg ctccatggtc caatcgggcc
      961 ggtcgccgca gatgcgaaaa acaaaattat cgagcacctg gcggccgcgc gcggaatgag
     1021 cgacctcggg atgaaactgc aaaccgaaaa agcgccgcgt cggatcggcg aaggaagcga
     1081 tcggcgagtt gtcgctatgg gctaggatct cccagccctt gggaaagctc gtcatcttgt
     1141 cgccatggct catccacacc tgagtcgtgt cacgtctgga aaaaccgtgg aacaaatcgc
     1201 tgtcgttgtc gatgaccagt tgcgccggtc cgtattcgcg ccggtcggcg cgcgctgcgg
     1261 caccgccgaa gtgcaggttc aaaacaccca tgccataaca gataccgaga atcggcaccg
     1321 gcagcgccaa cagctcagcg gaaacctttg gcgcgtcgtc ttgatagacg ctcgccggtc
     1381 cgcctgaaag gatgatccct ttgggatcga gcgcggttat tttctcgagg gcgacgttga
     1441 acggatggat ctcgcagtaa accttggact cgcggatacg gcgggcgatc agctgggtgt
     1501 actgagaacc gaaatcgagg acgacaatca ttctactctg tagttcggcg cttccttggt
     1561 gataaacaca tcgtgcacat gaccttcgcg cagcgctgcc gctgtgatct gcatgaattt
     1621 cgctttgcta tggagcgcgc cgatctcttt gcagccgaga tagcccatgc ccgcgcgcag
     1681 gccgcccacc agttgtaaaa tattcgacga caacgagcct ttgtacggca cccggccttc
     1741 gatgccttcc ggcaccaatt tgttggagtc cgtcacatcg gcctggccat aacggtcccg
     1801 gctgccctgc cccatggcgc cgatggagcc catgccacgg taggctttgt aggtgcggcc
     1861 ttgatagaga atggtttcgc caggactctc ttcagtccct gcgaacaggc tgccgatcat
     1921 caccgagtgc gcgccggcgg ctaatgcctt ggtaatatcg ccggaaaatt taatcccgcc
     1981 gtcggcgata atggcaacgt tatgcttggc cgccgccttg gcacaactgc caatggcggt
     2041 gatctgtggc acgcccaccc ccgagacaat ccgcgtggtg caaatcgatc cgggaccgac
     2101 gccgaccttg accacgttga cgccggcctg gatcaaggcg ctcgtgccct cgccggtggc
     2161 gacgttgccc gcagcgatgt cgagatcagg ataacgccgg cgaatctggc cgacgacatc
     2221 gaggacattt ttcgaatggc catgggccgt gtccaccgcg atcaaatcta cccccgcccg
     2281 caccagccct tccacccgcg gttcccaatc gctgacgccc accgccgcgc ctacgcgcag
     2341 cctgccgcgt tggtccttac acgcgccggg atattgaatg gtcttctgaa tgtctttgac
     2401 ggtgatcagc cccttgagct cgaagttgtc gtcgaccacc agtagtttct cgatgcgatg
     2461 gcggtgcagc aattctttgg cggtctcgag atctactccc ggccgcaccg tcaccaaatt
     2521 atctttggtc atcacctcgg aaactttgcg gtcgaggcgg cgctcaaagc ggagatctcg
     2581 attggtgaga atgccgacca gcttgccgtt cttagtcacc gggagaccgg agatgccgta
     2641 ctgggtcata attccctggg cttcggaaat tttttgctcc ggcgcgatgg tgatcggatc
     2701 gctgatcatg ccgctctcga attttttgac tttttcgacc tcggacgcct gggcccagac
     2761 cgaaaggttg cgatgaatga taccgagtcc gccctcttga gccatgccga tggcggtctt
     2821 ggattcggtc accgtatcca tcgcggcgct gaccagcggg atactgaggc ggatgcgatc
     2881 ggtcagccgg ctcgagacat ctatatcctt cggcagcacc gacgactcgg cggggatcag
     2941 caagacatcg tcgaaagtta gaccgacggg caggacttcc tgctctaaca taggtcacct
     3001 ccgcgccgaa ataaaaaaaa acccagacct gggcctgggt tggagacgca cacggaacgt
     3061 ttcaaagcaa acatgaagtc ttataccaag aaatattttt cacggcaaga acaggccaga
     3121 ttgagccact gctaacgata tggtaacaac taacaccgaa catgccgctt tacgaatacg
     3181 aatgcaccca aggccatcgc ttcgaagctc tgcaaaaggt ctctgataaa ccgctcaaga
     3241 aatgcgatca gtgccgcgcc gtcgccaaac gagcggtgtc gcagaccatc ttgctgcaca
     3301 atcacggcgt tcatgtcttc gaccgggtca ccggcgatga tgtgctgcgc ggccggccgt
     3361 cctccttgaa gtccttcaag aaagacaagt tttagcagtc tttccggcaa gattccgaca
     3421 gctgccgccg gcagacgcca gcatcgatgt cgatcctcgg tcccagtccg gttgtgctcc
     3481 cgagccgcct ttgttagcgt acggcacctt tacaaccgga tatgctcgcc aaggtcaata
     3541 gcgctgctct ttatggtatc gacgcactcc gcgtcgaagt cgagatcgat ctcgcctccg
     3601 gcttgccgca actagcgacg gtcggtttac ccgaaggcgc ggtcaaggaa agcaaggacc
     3661 gcatccgcgc ggctgtgaag aattgcggct acacctttcc cgccaagcgc atcaccatca
     3721 acctcgcgcc ggcggacatc aaaaaagaag gctcagccta cgacctgccg atggccatag
     3781 gcatactcgc cgccgagggc acgttagaca aaactctcct cgactcgtat tttctcatcg
     3841 gtgagctgtc cctcgacggc agcgtcaaac cggtgcgcgg cgcgctgtcg atcgccatcg
     3901 ccgccaaaaa agaaaaattt cgcgggctga tactccccga gcaaaacgcc tcggaagccg
     3961 cggtggtcag cgatctcgaa gtgctgggcg tgcgcgattt gccgcaggtg gtcgaatttt
     4021 tgaatggcaa ccggaccatc attcccaccg gcgccaacct ggacgatctg ttcagccagc
     4081 attctcaata caacgccgac ttcaacgacg tcaaaggcca gcagcacgtc aaacgcgccc
     4141 tggaagtcgc agccgccggg ggtcacaacg tcatcctggt gggtccgcct ggctcgggca
     4201 aaaccatgct ggccaaacgg ctgccgacca ttcttcccga tctgacattg gccgaagctc
     4261 tcgagaccac caagattcac agcgtcatgg gcaccctcgg cggccaagcg atcattgcca
     4321 cccggccgtt ccgttcaccg caccacacga tctccgatgc gggtctcgtc ggtggcggca
     4381 cggtgccgag accgggggaa gtctcgctcg cccacaacgg cgtcctgttc ctcgatgaac
     4441 tgccggaatt tcgccgcaac gtcctcgaag tcatgcgcca gccgttggaa gacggtagca
     4501 tcaccgtctc ccgcgccatg ggttcgatca actatcccgc gagcttcatg ctggtcgcgg
     4561 cgatgaaccc ctgcgtgtgt ggattcttca ccgacccgca gaaggaatgc atttgcacgc
     4621 cgctgcaaat tcagcgttac cgttcgaaag tctccggccc gctgctcgac cgcatcgaca
     4681 ttcagatcga agttcccggc ctgcgctatc aagaactcgc cagcaaagac tccggcgaga
     4741 gttcgactgt cattcgcgag cgcgtcaatc atgcgcgtaa aattcagttg cggcgttttg
     4801 aaaaaaccaa gattcacg
//
LOCUS       DASQKS010000010         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3469, whole genome shotgun sequence.
ACCESSION   DASQKS010000010 DASQKS010000000
VERSION     DASQKS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3469"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..904
                     /locus_tag="VE170_00230"
     CDS             98..904
                     /locus_tag="VE170_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYJ13884.1"
                     /translation="MSKFAIAQVQSSTDKAANLKQALRLIDEAKSQAAEIIAFPEFLM
                     AFSPGSQSAEELTLMAEAVDGPFITALRDRAKAAKLGVLAAIYESSPLANRVYDTAVW
                     IDAAGDIASVYRKLHLYDAFGFKESDKFHPGDDIAPLVTVGAAHCATMICYDLRFPEL
                     ARMLTLAGANVLFAPSGWVQGDLKIDHWRTMIKARALENGCFVFAPNQVGNIYTGHSM
                     AVDPLGRTLIDLQEKEELAVIEIDLALVNDVRDKLPLLHNRRTDVYAKHR"
     gene            complement(921..2216)
                     /locus_tag="VE170_00235"
     CDS             complement(921..2216)
                     /locus_tag="VE170_00235"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYJ13885.1"
                     /translation="MILKLLRTFIAEMFAGLPIGTAFCAAALALVFAFVPALNPSDGD
                     ERAAALPRTEHRLEERIAVKLGRNETLQELLNRFGLRPGSAQELAQKIYSSVDLRRMP
                     RDQAISLVVDRQDGNIRAVEFVMQEHLVHASLGSLGWSVERQELAHVEGLNIVRVRIT
                     ESFVQSAARAGISAAQIAGLQRVFRADLDLQADLGAGDEVLLIVPQKLYLNGYVAMAP
                     MAAVRVVHGGRFIDAFGLRGDDGALQYYDSDGHLLPRPFLAAPLKFDRISSTFDLARP
                     DPATGALRPHEAVDYQAAYGTPVVAIGAGMVEFAGVRPGNGLMVEIKHADGYTSSYSH
                     LSRLGERVEEGRRVNAGEVIGAVGQTGYATGPHLHFEFALDGEKLDYLSVKVPGAQSL
                     SGYQLTQFQSERAKWLAALRGSAVRIIQLPNSHWQGTSG"
     gene            complement(2399..2932)
                     /locus_tag="VE170_00240"
     CDS             complement(2399..2932)
                     /locus_tag="VE170_00240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HYJ13886.1"
                     /translation="MSAQPSETTAELLIRAERGADVTTIGKVVCAAFASVARSNQKEH
                     LLVDRLRESGALAVTLVAEVEGRVVGHVACSPVTIAGRFCDWYGLAPLAVLPSYQRTG
                     IGSRLVIAGLESLCALQAQGCVLLGEPEYYGRFGFSIRENLLLKKVPPEYFLARSFSN
                     DYPHGKVAYHPAFALCS"
     gene            complement(2929..>3129)
                     /locus_tag="VE170_00245"
     CDS             complement(2929..>3129)
                     /locus_tag="VE170_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HYJ13887.1"
                     /translation="HGLRANASTKSHKELDEATSLDAGDPIDLAKRYRECQTRLSQLN
                     VFGGCCGTDHRHIDAICRAILS"
BASE COUNT          758 a          929 c          806 g          636 t
ORIGIN      
        1 actcctgaaa atgaaccagg acccgtgtac cgattgacct agctaaccta cttggatagc
       61 ttgactggca acgataatcc aggctgaaag ataaaccatg agcaagttcg ccatcgccca
      121 agttcagtcc tccaccgaca aggccgccaa tctgaaacag gcgttgcggc ttatcgatga
      181 agccaaaagc caggccgccg aaatcatcgc ctttccggaa ttccttatgg cgttttcccc
      241 tggaagccag agcgcggaag aattaacact gatggcggag gccgtcgacg gcccttttat
      301 aaccgcgcta cgcgaccgtg ccaaagcggc caagctcggc gtgctggcgg cgatctatga
      361 atcgagccct ttagccaatc gcgtttacga caccgcggtc tggatcgatg ccgccggcga
      421 tatcgcgtcg gtttatcgca agctccatct ctacgacgct ttcggcttca aagagtcgga
      481 caaatttcac cccggcgacg atatcgcgcc gttggtcaca gtcggcgctg cacattgcgc
      541 cacgatgatc tgctacgatc tgcgattccc tgagctggcg cgcatgctca cgcttgcagg
      601 cgccaatgtt ttattcgccc catccggctg ggtccagggc gatctcaaaa tcgaccactg
      661 gcggacgatg attaaagcgc gcgccctgga aaacggctgc ttcgtgttcg cgcccaatca
      721 ggtcggtaac atttacaccg gccacagcat ggcggtggac ccgctcggac ggacattgat
      781 cgatctgcag gaaaaagaag aattagcggt aatagaaatc gaccttgcct tggtaaatga
      841 cgtgcgcgac aaactaccgc tattgcacaa ccgtcgcacc gacgtatatg cgaaacatcg
      901 ctagacgtcg gtgaagattt ttatccggaa gttccctgcc agtgtgagtt cggcaattga
      961 ataatcctaa cggcggagcc gcgcagtgcc gcaagccact tagcccgctc gctttgaaac
     1021 tgcgtcagtt ggtatcccga taacgattga gcgccgggga cttttacgga caaatagtcg
     1081 agcttctcac catcgagggc gaactcaaag tgcagatggg ggccggttgc atagccggtc
     1141 tggccgactg cgccgatgac ttcacccgca ttgacccgcc ggccctcttc gactctctcg
     1201 ccgaggcgcg acaaatgcga gtaactcgaa gtataaccgt cggcatgttt gatctccacc
     1261 atgagaccgt taccaggacg cacaccggca aactcgacca tgcctgcgcc gatcgctaca
     1321 accggggttc cataagcagc ctggtagtcg accgcttcat ggggacgcag cgcacccgtc
     1381 gccggatccg ggcgcgccag atcgaacgtc gaagagattc gatcgaattt taacggcgcc
     1441 gccagaaacg gccgcggcaa caagtgaccg tcgctatcgt agtattgcaa cgcaccatcg
     1501 tcgccgcgca aaccgaaggc atcgataaac cggccgccat ggaccacgcg caccgccgcc
     1561 atcggcgcca tcgccacata tccattgagg tataattttt gcggcacaat taacaaaacc
     1621 tcatcgccgg cacctaagtc agcctgcaaa tcgagatcgg ccctaaatac ccgctgcaat
     1681 ccggcgatct gcgccgccga aataccagcc cgcgccgcgc tttggacgaa actttcggtt
     1741 atccgaaccc gcacgatatt taatccttct acatgagcca gctcctggcg ctccaccgac
     1801 cagccgagcg agccgagact ggcgtgcacg agatgttcct gcatgacgaa ctcaacggcg
     1861 cgaatatttc catcctgtcg gtcgacgacc aaactgatgg cctggtctcg cggcatacgc
     1921 cgtaaatcga ccgaggaata aattttctgc gccaactcct gcgccgaccc cggacgaaga
     1981 ccgaaacgat tcagcagttc ttggagagtt tcattgcggc caagttttac cgcgatccgt
     2041 tcttccaaac ggtgttcagt ccggggtaac gcagctgctc tctcatcacc atcggatgga
     2101 ttcagtgccg ggacaaaagc gaagactaat gccagcgccg cggcacaaaa tgccgttccg
     2161 atcggcaggc cggcgaacat ctcggctata aaagtacgga gtagtttgag aatcatgagg
     2221 gagtagtcga aaggcgtttt gcaattttgt atgcgaaact ctcccagaga gcaagcgtgg
     2281 tctgtctaaa attgatctat tacttaacac gaaaacaatt aaggttttaa tttaacgcag
     2341 atacgaaatc gaaatccgcg aaataatttt tcaccacggc gactattttt tgaatcggtc
     2401 agctacacag cgcgaaggct ggatgataag caaccttgcc atgcggatag tcgttagaaa
     2461 aagagcgcgc taaaaaatat tccggcggaa ccttttttag cagtaaattt tcccgaatac
     2521 taaacccgaa ccgcccataa tattcgggct cgcctagtaa cacacagcct tgcgcctgca
     2581 gcgcgcataa agactccaac ccggcgatta ccaaacgcga gccgatgccg gttctctgat
     2641 aactcgggag taccgccagc ggcgccaatc cgtaccaatc acagaaacgc ccggcgatcg
     2701 tcaccggcga gcaagcgaca tgaccgacaa cccgaccttc gacttcggca accagcgtaa
     2761 ccgccagagc accgctttca cgcaatcggt cgaccagcag gtgttctttt tgattgctcc
     2821 gggccacgct ggcgaatgcc gcacaaacaa ccttgccgat cgtggtaacg tcagcgcctc
     2881 gttcggcgcg aatcaacaac tcagccgttg tctcgctggg ttgggcgctc atgagagaat
     2941 cgctcggcaa atcgcgtcga tatgccggtg gtcggtgccg caacaaccac cgaacacatt
     3001 gagttgactc aacctggttt gacactcgcg gtagcgcttg gctagatcga taggatcgcc
     3061 ggcatccaaa gatgtcgctt catcgagttc cttgtgactc ttggtcgatg cattggctcg
     3121 gaggccgtg
//
LOCUS       DASQKS010000011         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3784245, whole genome shotgun sequence.
ACCESSION   DASQKS010000011 DASQKS010000000
VERSION     DASQKS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784245"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..991)
                     /locus_tag="VE170_00250"
     CDS             complement(263..991)
                     /locus_tag="VE170_00250"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13888.1"
                     /translation="MASGTWRGIVGVVKPTYGSGSLVEFIRLLPEGVGCIPMYAGIKE
                     HSERGYLGAMENYHTKVAELAEIGVDVMHPEGGPPFMVRGHKAEQEIVSAWEAKYKVP
                     IFTSGMSQAQALRALGIKKFVGCTYFRDQKMNDVFTRYFTDCGFEVLGMEGMDTSPDE
                     ADGISNETIYRHLKASFNKHPTAQGIYLLGSGAWGVKDIIAAENDLGVPVVHPVAARV
                     WYVQKLLKIKNPLQGASRLLEELP"
     gene            1198..2004
                     /locus_tag="VE170_00255"
     CDS             1198..2004
                     /locus_tag="VE170_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYJ13889.1"
                     /translation="MTNRPTDTLTQEYGNLVPTGRLLDGKPRSILFDATKCIGCRHCV
                     QACKDWNDHDRTSAYELSSTNWITMEPPVLEGMSPIWARNSCMHCDFAACAAVCPVEA
                     ITKYEEGPVVINKDVCIGCEYCIHACPWEVITKNEITGKASKCTMCSDRISEDKQPFC
                     VQACPVNALDFGFTEEIADKAKKQAEAVGGYTYGDKEAGGGSVVYVLKESKDKYGVRE
                     IAAGRFPKHKIPLGLMLKDLFTPRCGVAGKMRALWLALAHPKRLLYRYFS"
     gene            2188..3162
                     /locus_tag="VE170_00260"
     CDS             2188..3162
                     /locus_tag="VE170_00260"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13890.1"
                     /translation="MAIFLAAIMFWSPTIAHGEKIRSAIPQANLNYLSIHVADARGFF
                     KDEGLENETVVISGPLATAALLSGDVDFSGGGGSGMRAALKGAPVKGIYFQTEKVTFY
                     LVADPSIRTAADLKGKRIAVGSPGDTQDRMMTMFAERGGVASREMIRISMGSDTNTRI
                     TAIKTGAAHAAIVDPGGLVFAQKEGLTSLGFLGDLFPMPFQGFVTTDRKLRENPAQVK
                     RWLRAAIRALMFVRDRPEDAVDLGMKKLQLGRASKAMIVEGTKNYVRALPQGVPGMPS
                     AEGIKNFLEYDIKIPLGFKEDIAPERLLHLQLVKEVKEELEAAQRRSK"
     gene            3163..>3855
                     /locus_tag="VE170_00265"
     CDS             3163..>3855
                     /locus_tag="VE170_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ13891.1"
                     /translation="MATEQQLREQLATCTRIFAMQGLIGLFGHVSVYQPETKTIFVTP
                     GSGSDKAHFTASDMIPIDLTGKPTTGTIPPPVEWPIHTALHGARPDALAVAHLHTPFA
                     TLFAIAKREFHPVTLQGTVFGEGVPLYKEAHLVTTPQRGDAMLKTIGDRRALLMRGHG
                     IVVAGKSLAEVMFASLILEDDAKKTMQAATLGSVEHFSADECKTFNAEIDFERRANRA
                     WEFFSRIESQWDK"
BASE COUNT          888 a         1169 c         1037 g          761 t
ORIGIN      
        1 ctgcccggca tgatctggga ttactacaaa caatccggcc ggcacgagcg cacgggcaga
       61 cccttcaggg acacgctgga aaaactcggc ctgcaagagt ttcaggaatg gagccaactc
      121 gattaaaaaa atctagcatg ctggacggtt ggagcgaagc aagacatcgt tcatgcgcat
      181 cggcaaggct agcgcggcag gcaaatcttc gttacttcgt ccgaattacg tcgctcgatc
      241 cccgcccttg gaccacgctt ccctacggca gttcttccaa cagccgactc gcgccctgta
      301 acggattttt aatcttcaat aatttctgca cgtaccatac ccgcgcggca actggatgaa
      361 caaccggaac gcccagatca ttttctgctg cgatgatgtc tttcacgccc caggccccgg
      421 agccgagcaa ataaattccc tgcgccgtcg gatgtttgtt gaacgacgct ttgagatgcc
      481 ggtaaatcgt ttcgttggaa attccgtccg cttcatccgg tgaagtgtcc atgccctcca
      541 tgcccaacac ctcgaacccg cagtcggtga agtaccgcgt aaaaacatcg ttcattttct
      601 gatcgcggaa ataggtgcag ccgacaaact ttttgatgcc caaggcgcgc agcgcttggg
      661 cctgtgacat cccagatgta aagatcggca ctttatactt ggcctcccaa gcgctgacga
      721 tctcctgctc ggccttgtgg ccgcgcacca tgaacggcgg cccgccctcg ggatgcatca
      781 catcgacgcc gatctccgcc agctccgcga cctttgtgtg ataattctcc atggcgccga
      841 gatagccgcg ttccgaatgt tccttgatgc cggcgtacat cggaatgcaa ccgacacctt
      901 ccggcagaag tctaataaac tccaccaacg atcccgagcc ataagtcggt ttcaccacac
      961 cgacgatgcc gcgccatgtt ccggaagcca tgtgttatct cccatccagt taaaatcgaa
     1021 tattaatggc atgctagtcg ggacacgcaa caaaaaacaa gagcgatccg cgcaccagca
     1081 ttattccatt cttcattctc gaccctcgag cctcgacccc aatacacagc ccttcagttg
     1141 acggcgccaa aaggtaagtg atacacacac tctaaaagta agcgcgaaag gatcagaatg
     1201 acgaaccgac ccacggacac actcacacaa gaatacggca acctggtgcc caccggccgg
     1261 ctgctcgacg gcaagccgcg cagtatctta ttcgacgcaa ctaagtgtat cggctgccgc
     1321 cactgcgtcc aggcgtgcaa agactggaac gatcacgacc gtaccagtgc ctacgaactc
     1381 tccagcacca attggatcac catggaaccg ccggtgctcg aggggatgtc gccaatctgg
     1441 gcgcgtaaca gctgcatgca ctgcgacttc gccgcctgcg ccgcggtttg tccggtcgag
     1501 gcgattacca agtacgagga aggaccggtg gtcattaaca aagatgtctg catcggctgc
     1561 gaatattgca tccacgcctg tccctgggaa gttattacca agaacgagat caccggcaag
     1621 gccagcaagt gcaccatgtg cagcgaccgc atcagcgagg acaagcagcc gttctgcgtc
     1681 caggcgtgcc cggtgaacgc gttggatttt ggcttcaccg aggagatcgc cgacaaagcc
     1741 aagaagcagg ctgaagcggt gggcggctac acttacggcg acaaagaagc cggcggcggc
     1801 agcgtcgtct atgtcctgaa agaaagtaaa gacaaatacg gcgtgcgtga gatcgccgcc
     1861 ggcagatttc ccaaacacaa aattccgcta ggcttgatgt tgaaggacct gttcacgccg
     1921 cgctgcggcg tcgccggcaa aatgcgcgct ctttggctgg cgctggctca cccgaagcgg
     1981 ctgctctacc ggtatttctc ttaacctcga ttacaactgc gcaatgataa ctcaaatcga
     2041 ccgcttcctt gcttcggcat tgttgcggag gccggaatgc cgctccttct gcgcccggat
     2101 tctcggtaga gatagctatc gccagcgcgc ccggtttgac gacggtaata aactatgaag
     2161 tgttttgtca tttccggttg tttcatcatg gcaattttcc tcgcggcaat tatgttctgg
     2221 tcgcccacca tcgcccatgg cgaaaaaatt cgcagcgcga ttcctcaggc aaatctaaac
     2281 tatctgtcca tccacgttgc cgacgcccgc ggctttttca aagacgaggg cttggagaac
     2341 gaaaccgtgg tcatttccgg tcccctcgcc accgccgcat tgcttagcgg cgacgtcgat
     2401 tttagcggcg gcggcggcag cggcatgcgc gccgcgctga aaggcgcgcc ggtgaagggc
     2461 atctattttc agacagagaa ggtcaccttc tacctggtcg ccgacccgag catcaggacc
     2521 gccgccgatt tgaagggcaa aagaatcgcc gtcggcagcc ccggcgacac ccaggaccgc
     2581 atgatgacga tgttcgccga acgcggcggc gtggcatcgc gcgagatgat tcgcatctcg
     2641 atgggctcgg atacaaatac gaggatcacc gcaatcaaaa ccggcgcggc ccacgccgcc
     2701 atcgtcgatc ccggcggcct cgtcttcgca caaaaagaag ggctcacttc gctgggtttt
     2761 cttggcgact tgtttccgat gcccttccag ggcttcgtca ccaccgaccg caagttgcgc
     2821 gagaatcccg cccaggtgaa acgttggctg cgcgcggcca tccgggcgtt gatgttcgtg
     2881 cgcgaccggc ccgaagacgc ggttgatttg ggcatgaaga aattgcaact gggccgggcg
     2941 tcgaaagcga tgatcgtcga aggcacgaag aactatgtgc gcgccttgcc ccaaggcgtg
     3001 ccaggcatgc cgtcggccga agggatcaag aactttctcg aatacgatat aaaaatccca
     3061 ctcggcttca aagaagatat agcgccggaa cggttgctcc atttgcagct ggtcaaggaa
     3121 gtcaaagaag aactggaagc ggcacaaagg agatccaagt agatggcaac ggaacagcag
     3181 ctacgcgagc aactcgcgac ttgtacgaga atattcgcca tgcaggggct gatcggcctg
     3241 ttcggccatg tcagcgtcta tcagcccgaa actaaaacta tcttcgtcac tcccggttcg
     3301 ggctcggaca aggcgcactt cacagcatcg gacatgatcc ccattgatct gaccggcaag
     3361 ccgaccaccg gcacgatacc gccgccagtc gaatggccga ttcacacggc gctccacggc
     3421 gcacgcccgg atgcgctggc cgtcgcccat ctgcacactc ccttcgcgac tctatttgcc
     3481 atcgccaagc gcgaatttca cccggtgacc ttgcagggaa cggtgttcgg cgagggcgtg
     3541 ccgctctaca aggaagccca tctggtcacc acgccgcagc gcggcgacgc catgctgaaa
     3601 accatcggcg atcgccgcgc cctgctcatg cgcggccatg gcatcgtcgt cgccggcaag
     3661 agtttggcgg aagtcatgtt tgcgtcattg atcttggaag acgacgccaa aaagaccatg
     3721 caagctgcaa cgctcggttc ggttgaacat ttttccgccg atgagtgcaa aactttcaac
     3781 gccgagatcg atttcgaacg acgcgccaac cgggcttggg aattcttctc gcgcatcgaa
     3841 tcgcagtggg ataaa
//
LOCUS       DASQKS010000012         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3365829, whole genome shotgun sequence.
ACCESSION   DASQKS010000012 DASQKS010000000
VERSION     DASQKS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3365829"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VE170_00270"
     CDS             complement(<1..984)
                     /locus_tag="VE170_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13892.1"
                     /translation="MNLIERMAILMVTVLALLQWHPSEAGQAKAMIKVVAGYGSTDGA
                     IAPLWFAKETRLFEKRGLDVVLVGMGTGAVSLRALIAKDLEIASLSGSGLVQAALQGA
                     DTVILSAALNGFVFKVFGAPEMTNPAQLKGKKLGVSRYGATSDFAIRLALKKWGLNPE
                     RDVSILQIGTTQDTVRAMQTKMIDAGVLSGTASLVARKAGFRELGDLAELGLHYPMAP
                     IGTTRAFLQKNEAVVKEFMLAYIEAIHDFKRNKETALAVLKKYSRNDDREILEDSYNN
                     YANKYLPLPIPTIDGIKTILAELGSTMPAAKNADPEQFVANKIAREIEASGF"
     gene            complement(995..1750)
                     /locus_tag="VE170_00275"
     CDS             complement(995..1750)
                     /locus_tag="VE170_00275"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13893.1"
                     /translation="MTLVTDKSYGWRGRLGNISPTACTEIFPYEFYRVAPDGVTLVLS
                     NLVIRDARESSQVESSWLGFEDAIASLRATRVDHITLAGAPLVLAKGVERYRELLSWL
                     RQQSPVPATTSPQAFADGLKHLGATKLAVATSFIPAHNQLVNEFLSAEGFTVLAVEGL
                     DTGMTSIEKAMLSPRQVFDHVRSVGRRNPTADAMLITSSAWPTLAVIQALEDELGRPV
                     VSSATGQIWAPFRDLGIKTAIRGYGKLLGTLAA"
     gene            1844..2824
                     /locus_tag="VE170_00280"
     CDS             1844..2824
                     /locus_tag="VE170_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13894.1"
                     /translation="MTVLRKSYSPIAVLLTFGWLLTPGATWLFAQEKKIVIGYTARDL
                     NNFPLFAAQARGFFKDAGKEVELVQIRSNIGLSGLLGGSVDYYTSFSSAVTLAAQQGS
                     PIVGIFSMIERPSLYMIARPEIRAMAELKGKTVGLGSIGGITVTITRRMLANYGVSAS
                     EFTIVSSGDLPVRLAAVKSGAIHATLAGPPAPVQAKELGLNVLASAADTVDFPLAGLS
                     TAVAKIRANRAEVVAVLTAMLRGLLFVRNQRSDAVGLIQKVFKMERELAEAGYDQSLP
                     AYSKDGTASARGIESVLEVARQGGAAKSVGVNDVVDFTPLREAQTLVKLR"
     gene            2897..>3483
                     /locus_tag="VE170_00285"
     CDS             2897..>3483
                     /locus_tag="VE170_00285"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYJ13895.1"
                     /translation="MSAMSKVQSLEAAIATHVRDGMSIAMGCGLESLIPFAASHEIIR
                     QGRRELTLIGPISDVQFDQLIGAGSVRKIQASWVGNVAAGLGHNFRRAAEHGQPLPLE
                     IEEHSNFTIGLALQAAAMGLPFLPTRSIKGSDIAHEPHFASVHCPFTGEELIAVRAIR
                     PDLAIVHVQRADDQGNAHLWGNYGVIREAAMAAAKV"
BASE COUNT          737 a         1025 c          999 g          722 t
ORIGIN      
        1 aaacccgctc gcctcgatct cacgggcgat tttgttagcc acgaattgct caggatcggc
       61 gtttttggct gccggcatgg tgctgccgag ttccgcaaga atagtcttaa tgccgtcgat
      121 tgtcggtatc ggcaggggca aatatttatt cgcgtagtta ttgtagctat cttccaaaat
      181 ttcccggtcg tcattacggc tgtatttttt cagcaccgcc aacgcggttt ctttgttgcg
      241 cttgaaatca tggatcgcct cgatgtaggc gagcatgaac tccttcacca ccgcctcgtt
      301 tttttgcaaa aacgctctgg tcgtgccgat cggcgccatc gggtagtgca agcctaactc
      361 ggccagatcg cccaactcgc gaaaaccggc tttgcgcgcg accagcgacg ccgtgccgct
      421 cagcacaccg gcatcgatca ttttcgtttg catggcacgg acggtgtcct gggtcgtgcc
      481 gatctgcagg atgcttacgt cacgttcagg attgagcccc cacttcttga gcgccagtcg
      541 gatggcgaag tcggacgtcg cgccatagcg gctgacgcct aatttcttgc ccttgagttg
      601 cgccgggttg gtcatttccg gcgcgccgaa aaccttgaaa acaaatccat tgagagcagc
      661 cgagagaatc accgtgtctg cgccttgcaa ggctgcttga accaatccgg aaccggacag
      721 tgaagcgatt tcgagatcct tggcgatcag cgcgcggagc gaaaccgccc cggtgcccat
      781 accgaccaac accacatcca agccgcgctt ttcaaataac ctggtctcct tggcaaacca
      841 caatggtgcg atggcgccgt cggtggaacc atagccggcg acgactttga tcattgcctt
      901 ggcctgcccc gcctcgctcg ggtgccattg cagcaaggcg agaaccgtga ccatcaggat
      961 agccatccgc tcaatcagat tcatttccgc cctcctaggc agctagggta ccgagcagtt
     1021 tgccgtagcc gcgaatcgcc gtcttaatac cgaggtcgcg aaacggcgcc cagatttgcc
     1081 ctgtcgcact gctcaccacc ggtctgccaa gctcatcttc caaggcctgg ataactgcca
     1141 gcgtaggcca agccgacgag gtgatcagca tggcatcggc ggtagggtta cgccgcccaa
     1201 cgctgcgcac atggtcgaac acttggcgcg gcgacaacat ggctttttca attgaggtca
     1261 taccggtatc gagtccttcg acagcgagga cagtaaaacc ttccgcgctg agaaactcat
     1321 tgaccaattg gttgtgagcc ggaatgaaag aagtcgccac cgcgagctta gtggcgccaa
     1381 gatgcttcaa gccgtcggcg aacgcctgcg ggctggtggt ggcgggcacc ggcgattgct
     1441 ggcgcaacca gcttaacaat tcccggtagc gctccacacc tttcgccaat accaagggtg
     1501 cgccggccag ggtaatgtga tcgacgcggg tcgcgcgcag gctggcgatg gcgtcctcga
     1561 atcccagcca agaagattca acctggctcg actcgcgagc gtcgcgaatg accaaattgc
     1621 tcaataccaa cgtaacgccg tccggcgcca cccgatagaa ctcgtaagga aatatctccg
     1681 tgcaggcggt cggtgagata ttgccgagcc ggccgcgcca gccataactc ttatcggtga
     1741 ccaaagtcat ggtcttataa gtgccccaaa agaccatacc aatgcaaagg gttttatgtc
     1801 ttatatcagt cgcaggccct aaccaaccaa ggagatgcga tcaatgactg ttctgcgaaa
     1861 atcctactcg ccaatcgccg tgctgctaac gtttggatgg ctgctcactc cgggcgccac
     1921 ttggttattt gcccaggaga aaaaaattgt catcggctac accgcgcggg atttgaataa
     1981 cttcccgctg tttgccgctc aagcgagagg atttttcaaa gacgcgggga aagaagtcga
     2041 gctcgtgcag attcgctcga acatcggact gtcgggatta ctcggcggtt cggtggacta
     2101 ttatacgtca tttagcagtg cggtgacctt ggcggcgcag cagggttcgc ctattgtcgg
     2161 catcttcagc atgatcgagc ggccgagcct ctacatgatc gctcgcccgg agattcgcgc
     2221 catggccgag ctcaaaggca aaacggtagg cctcggctca atcggcggta tcaccgtgac
     2281 catcacccga agaatgttgg ccaactatgg tgttagcgcc agtgaattta ccatcgtgtc
     2341 atcgggcgat ttgccggtgc gcttggctgc cgtaaaatcc ggcgcgattc acgcaacgct
     2401 ggccggcccg ccagcgccgg tgcaagctaa ggagctagga ctcaatgtgc tggcctcggc
     2461 cgcggatacc gtggactttc cattggcagg gctgtcgact gcggtggcga aaattcgcgc
     2521 caaccgcgcc gaagtggtcg ccgtgctcac ggcgatgctg cgcggcttgt tgttcgtgcg
     2581 caatcaacgc agcgatgccg taggcttgat ccagaaagtc ttcaagatgg aacgcgagct
     2641 cgccgaagcc ggctacgacc agtcgctgcc ggcttacagc aaagacggca cagcttcagc
     2701 gcggggcatc gaaagtgtac tcgaagtggc gcgccaaggc ggcgcggcaa aatccgttgg
     2761 ggtcaacgac gtggtcgatt tcacgccgct tcgggaagcg cagacgttag tgaaattgcg
     2821 ctgatacaaa attgcgcata gctccgtggc cgcttgagcc aaaccgcgtt gaagattagg
     2881 cagtgaaacg gtacagatgt cggcgatgtc aaaagttcag agcctggaag ccgctattgc
     2941 cacccatgtt cgtgacggca tgtcgatcgc gatgggttgc ggtctggaat cgctgatccc
     3001 cttcgccgcg agccatgaaa tcatccgcca agggcgcagg gaattaactt tgatcggccc
     3061 gatttcggac gttcaattcg atcaactcat cggtgccggt agcgttagga aaatccaagc
     3121 gtcgtgggtg ggcaatgtcg ccgccggcct tggccacaat tttcgccgag cggccgagca
     3181 cggacaaccg ctcccgctgg aaatcgaaga gcactcgaat tttaccatcg gcctggcgct
     3241 ccaggctgcg gcgatgggcc tgcccttcct gccgacgcgg tcgatcaagg gcagcgatat
     3301 cgcccacgaa ccccatttcg cgagcgtgca ctgtccattt accggcgaag agctcatcgc
     3361 cgtgcgcgcg attcgacccg acctcgcgat cgttcatgtg caacgcgccg acgatcaggg
     3421 caacgcgcat ctttggggca actacggggt catccgcgag gccgcaatgg ccgccgccaa
     3481 ggt
//
LOCUS       DASQKS010000013         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5885730, whole genome shotgun sequence.
ACCESSION   DASQKS010000013 DASQKS010000000
VERSION     DASQKS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5885730"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="topA"
                     /locus_tag="VE170_00290"
     CDS             complement(<1..1385)
                     /gene="topA"
                     /locus_tag="VE170_00290"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HYJ13896.1"
                     /translation="MPAKNLVIVESPAKAKTLEKYLGRDFQVKASVGHLIDLPKSKLG
                     VDIKKDFAPDFTVIQGKKKVIDDLKKAAKGKENIYLASDPDREGEAIAWHIRSQVVKS
                     HKHVHRVLINEITKKAVQAAIANPLTLDRNKFDAQVARRVLDRLVGYKISPILWTKVR
                     RGLSAGRVQSVAVRLVCEREREVRAFEPVEYWSLTALLEGRLPPPFKAKLVQWKNQKV
                     DNKKFRLENDATVQSIVKSLEGAAWVIGEVEKKERRRFPAPPFVTSKLQQEASRKLGF
                     QPKRTMQLAQQLYEGVELGSEGSVGLITYMRTDSTRISDDAIGAVRGFIQGQYGKNYV
                     PGKPNTFRSKKGAQDAHEAIRPTSTEYTPDKVRRYLRRDMFQLYSLIWDRFVASQMVP
                     AVYDQTAFEIPVNEAVFRATGQQIKFDGFMKVYIEGRDERANQNNDDDEDEAQDLDGV
                     LPDLQKGDALTL"
     gene            complement(1431..2585)
                     /gene="dprA"
                     /locus_tag="VE170_00295"
     CDS             complement(1431..2585)
                     /gene="dprA"
                     /locus_tag="VE170_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYJ13897.1"
                     /translation="MIQPSLSFAEEVSSTVMSGDYAPWLALARVKGLGCVSFKKLVAH
                     FGDPTRALSVARSELAAIDGLQRDAIDGIVGFSQWAEVDIDLKRIANAGITVVRYTDA
                     VYPARLRTIADPPPVLYVKGALTEADDKAVAIVGSRSASDYGRRVARDLARGLASFGF
                     TVVSGMARGIDGMAHETALNCHGRTIAVLGSGVERAYPPEHHKLYQRIAEQGAVISEF
                     PMATKPMAFNFPARNRLISGLSLGVVVVEATEKSGSLITASLAVDQGREVFAVPGEVG
                     SSRSRGAHRLIRQGAKLVETVDDIIEEIAPQLARSSGSTGKTEARQLPHNSGEAVQKI
                     FALLQARSLQIDEIIESSGLGSAQVLGILLDLELQGYLRQLPGKVYTIER"
     gene            complement(2778..>3453)
                     /locus_tag="VE170_00300"
     CDS             complement(2778..>3453)
                     /locus_tag="VE170_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYJ13898.1"
                     /translation="GIRRKARIDDRIERFSVSRSLNAVERGDLIIHIIDGPENVTDQD
                     AQILSYAVQRGKAILLVVNKWDMVRETGGGTDKYRDEINYRLAFLEYVPIAFISAATG
                     FGVRKMLETAARLVQVYRRKVSTSSLNQALQDIVQAHTVPLFRNHPVKFYYGTQTGVA
                     PPTFTFFVNKPDGILESYQKYLVNQLRQQLGFNNAPIRLHFRARREPSTIKRSGNSPK
                     RSRSAR"
BASE COUNT          724 a         1016 c          938 g          775 t
ORIGIN      
        1 agcgtgagcg catcgccctt ttgcaggtcc ggcagcacgc catcgaggtc ctgcgcttca
       61 tcctcgtcgt cgtcattatt ctggttggcg cgctcatcgc gtccctcgat gtagactttc
      121 atgaagccgt caaacttgat ctgctggcca gtggcgcgga acaccgcttc gttgaccgga
      181 atctcgaagg cggtctgatc gtacactgcc ggcaccatct gactggcgac gaaacggtcc
      241 caaatcaagg aatagagctg aaacatgtcg cggcgcaggt aacgccgcac tttatccggc
      301 gtatattccg ttgacgtcgg ccggatcgcc tcatgggcat cttgcgcgcc tttcttggag
      361 cgaaaggtat tgggctttcc cggcacgtag tttttgccgt actggccttg gatgaaaccg
      421 cggacggcgc cgatggcatc gtcggagatt cgcgtcgaat cggtacgcat gtaggtgatc
      481 aaccccaccg atccttcgct gccgagttcg acgccctcgt aaagctgctg cgccaactgc
      541 atggtgcgct tgggttgaaa ccctaactta cgcgacgctt cctgttgcag tttgctggtg
      601 acgaacggcg gcgcggggaa acggcggcgc tctttctttt cgacctcgcc gatgacccac
      661 gccgcgcctt ccaaagactt gacgatcgat tgcacggtcg cgtcattttc cagacgaaac
      721 tttttgttat cgaccttttg gttcttccat tggaccagtt tggctttgaa cggcggcggc
      781 aaccggcctt ccaggagcgc ggtgagcgac caatactcga ccggttcgaa ggcacgcact
      841 tctctctcgc gctcgcaaac caatcgtact gccactgact ggacccggcc ggcgcttaga
      901 ccgcgccgga ccttggtcca caagatcgga ctgatcttat aaccgaccag acggtcgagc
      961 acccggcgcg ccacctgggc gtcaaattta ttgcgatcca acgtcaacgg attggcaatc
     1021 gccgcctgca ccgctttctt ggtgatttcg ttgatcagca cgcgatgtac atgcttgtga
     1081 cttttcacca cttggctgcg aatatgccag gcgatcgctt cgccttcgcg atccggatcg
     1141 gatgccaggt aaatattctc tttgcccttg gcggcttttt tcaaatcgtc gatgactttt
     1201 ttcttgcctt gaatgaccgt gaagtcgggc gcgaaatctt ttttgatatc gacgccgagc
     1261 ttgctcttgg gcagatcgat caaatggccc accgacgcct tgacttggaa atcccggccc
     1321 aaatattttt ccaaggtctt ggctttggca ggcgattcga cgatgacgag gttctttgct
     1381 ggcataatcg ttaactcaca atgggaactg acgacgctta cggtctggcg ttatctttcg
     1441 atcgtataga ctttgcccgg taattgcctc agatatccct gcaactccag gtccaataga
     1501 attccaagca cttgcgcgga tcccagcccg ctgctctcaa taatttcatc gatctgcagc
     1561 gaacgcgctt gcagtaaggc gaaaattttc tgcacggcct caccgctgtt atgcggcagc
     1621 tgtcgcgctt cagttttgcc ggtggagccg gacgagcgcg ccaactgggg cgcgatctct
     1681 tctataatat cgtcaacggt ctcgaccagt ttcgcccctt ggcggatcag ccggtgcgcg
     1741 ccgcggctgc ggctagagcc gacttcaccg ggcacggcga acacttcgcg cccctgatcg
     1801 acagcgagcg atgcggtaat caaggaacca cttttttccg tcgcctcgac caccaccacg
     1861 cctaacgaca agccactgat caagcgattg cgcgcgggaa aattaaacgc cattggcttg
     1921 gtggccattg gaaattcgga aatcaccgcg ccttgttcgg cgatgcgctg ataaagctta
     1981 tggtgctccg gcggataggc gcgctcgaca ccggagccca acaccgcgat ggtgcgccca
     2041 tggcaattca atgcggtttc atgggccatg ccatcgatac cgcgcgccat gccactgaca
     2101 acagtgaaac caaacgacgc caagccgcgc gccaaatcgc gcgcaacgcg ccggccatag
     2161 tcgctggcgc tgcgcgagcc gacaatcgcc accgccttgt cgtcggcctc ggtcaatgct
     2221 cctttgacgt aaagcaccgg cggcggatcg gcgatggtgc gcagccgcgc cggatagacg
     2281 gcgtcggtat aacgcaccac ggtaatacct gcgttagcaa tgcgcttgag atcaatgtca
     2341 acttcggccc actggctaaa gccgacaatg ccgtcgatgg catcgcgctg caggccgtca
     2401 atggcagcga gctcgctccg cgccaccgat aaagctcggg tcggatcgcc gaagtgggcg
     2461 acaagctttt tgaagctcac acagcccagc cctttgacgc gcgctaacgc aagccacggc
     2521 gcataatcgc ccgacatcac tgtcgatgag acttcttcag caaatgataa cgacggttga
     2581 atcatggtcg tccgcgctct atcgaaccct gacctcggcc aacgagtcac gaatgataaa
     2641 ataaatgtcc gacgaaggct aggcagtaga cgaaaatgat ccgccaatca agtaaaagcc
     2701 gttgttttta cgaatctttc tagtgcgtgg cgcagtcgat ggttatgcgc caagcggcat
     2761 cgacgagctg caatcgacta tcttgccgag cgcgatcgct tgggcgaatt accgcttcgt
     2821 ttaatcgttg acggctcgcg ccgggcgcga aagtgcagcc gaatcggcgc attgttaaag
     2881 cccaactgct gacgaagctg gttgaccaga tatttttggt agctttccag gataccgtcc
     2941 ggcttgttga cgaaaaacgt aaatgttggc ggagcgacgc cggtctgtgt gccgtaataa
     3001 aacttcactg gatggttgcg aaaaagcggc acagtgtgag cctggacgat atcttgaagc
     3061 gcctggttta gcgaagatgt cgagaccttg cgccggtaga cctgcaccaa ccgcgccgcg
     3121 gtttcgagca ttttgcgcac accgaaaccg gtcgccgccg aaatgaacgc gatcggcaca
     3181 tattcgagaa acgccaagcg gtaatttatt tcatcacgat atttatcggt gccgccgccg
     3241 gtttcgcgaa ccatatccca tttattgacc accaataaaa ttgccttgcc gcgctgcacg
     3301 gcataactca gaatttgcgc atcctggtcg gtgacgttct ccgggccgtc gatgatatga
     3361 ataattaaat cgccgcgctc gacggcattg agcgaacggc tgacgctgaa acgttcgatc
     3421 cgatcgtcga tgcgcgcttt gcgccgaatc ccg
//
LOCUS       DASQKS010000014         6861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5887342, whole genome shotgun sequence.
ACCESSION   DASQKS010000014 DASQKS010000000
VERSION     DASQKS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5887342"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VE170_00305"
     CDS             <1..349
                     /locus_tag="VE170_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ13899.1"
                     /translation="KERDFVTAAKALGARDAYVLFHHVVPNTLASVTVIATFGMAIVI
                     ITEASLSFLGLGVPVHIPTWGSMLAEGRSYINRAPWLTIFPGLAIFITVFGINLLGDG
                     LRDIFDPKLRRQR"
     gene            381..1919
                     /locus_tag="VE170_00310"
     CDS             381..1919
                     /locus_tag="VE170_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13900.1"
                     /translation="MLKKRSALSRRQFLTRSAAMAVGAAVGPYFLRNAGAASRDRVTI
                     FHSSVADSIHPYNHSSSPIYGNWQHVIEPLIEYDVGRKDFIGVLAESWQFQGNKWVFK
                     LRKGVKFHNGATFTSKDVVFSVDKMRDEKGGSLQAPNFKDVSEIQTPDDLTVVFVTKQ
                     PNAVFLDRLDNRFMLSKEAGDKFGDKIYENLVGTGPYKFVSYQRGGNMVFTRNDDYWG
                     GKAAIKEVVFKKVTEDAARLAALESGQADFINNVPDHEVARLKNHPRVRIDQVEGLRM
                     FFLAINFAFKPWDNKLVRQAANYSVDAPAIVRNIFDGIGYPCNGPVGSTVLGVDPKHK
                     RYPYNPQKAKELLTQAGFPNGCDIQLYYSSGRYPKDREVCQVIAAQMVKGGFRVELIS
                     QEWALFWDKQGVNGGRLPFYYIGRGSLADADTLYDQYFRTGTTKRTNYSNAELDKLIE
                     EQQRTPDTKKRVQILQRAGKFIMDEALFIPLYNLADIYGVVRNLDWKKRADEKVKAWD
                     MKIK"
     gene            1972..3498
                     /locus_tag="VE170_00315"
     CDS             1972..3498
                     /locus_tag="VE170_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13901.1"
                     /translation="MSNGKINRREFLKHSGVAGCAALLGGAVVKLSYAASRERLTALS
                     SIGLDTLHPYAHSSSPQYGIWHNMIEPLVEVDYAKRTYYGVLAESWEFQGKRWAIKLR
                     KNIRFHDSSPFTAKDVVYSINRMKNDKLSLQRENFRDFTEVQAADDYTVVITTEQPVA
                     VMLDRLQNRFILSKTGMEAQGDQAELKPIGTGPYKFQSWQRDGNLVMVRNDSYWGAKP
                     SIREIVLRRVREDSGRVAGLLAGQGDVTNNVPVEELSRFDNHPRIRAEKVEGVRMYFL
                     AMNVTHKPFDNKLVRQAINYAVDPAVIIKHIYEGNGYIMNGPMGSNVIGFDPKIKRYP
                     LDLNKAKELLTKAGYPNGLDVKLYFSPDRYPKAKEVCQVIADQLSKVGIKAELVSQEF
                     VVFWGKEGVNGGKLGFYYVGRPAADADTVYDQYYRSGVSPRIQYKNPEFDKLVDEEQK
                     TGDAKKRLAMLHQLGRILMEDVPFCPLYTLAEIYGVARNINWKARPDEKLLYAEMKIR
                     "
     gene            3533..5068
                     /locus_tag="VE170_00320"
     CDS             3533..5068
                     /locus_tag="VE170_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13902.1"
                     /translation="MKRTSNDIDRRRFLKYGALAGVGALAGAAWPGSSWSASKERLTI
                     LSSIALDNLHPYAYSSGPQYGIWYNMIEPLVDVDFAKRSYFGVLAESWEFQGKKWIFK
                     LRKNVRFHDGSRFTAADVIYSFNRVKNDRLSLQKENFRDLVEMQALDDHTLVFVTEVP
                     NAVFLDRLENRFIISKSATEAQGGEPGEHKPVGTGPYRFVSWQREGNLVMTRHDNYWG
                     TKPAVKEIVIRRVREDAGRVAGLLAGQGDVINNVPVEELSRFDKHAKVRAEKVEGQRM
                     YFLAMNVTHKPFDNKLVRQAINYAVDATTIVKHIYEGNGYVMNGPMGSKVIGFDPNIK
                     RYPVDVKKGKELLDKAGHPNGVDVKLYFSPDRYPKAKEVCQVIADQLAKIGVRVELVS
                     QEFAVFWGKEWVNGGKSPFYYVGRPATDADTVYDQYYRSGVSPRIQYKNPEFDKIIDE
                     EQRTGDAKKRIALLHQAGRILMEDVPFVPLYTLAEIYGTARNVGWNARPDEKILAGGM
                     KIL"
     gene            5277..5822
                     /locus_tag="VE170_00325"
     CDS             5277..5822
                     /locus_tag="VE170_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13903.1"
                     /translation="MATGILNAVPPAAARMRYASTLRCFGQFLEGTELKAVEIKTHGD
                     DFILQAWNKGQSMAMDLDKLFTPLDVQKLEADGHAKRKPFAGPPNMLSLPQVLRLAGN
                     YVDRQHGRLLRVSWQDQSDKIQSITVQWEASPAARESSDAQLSVIEELCIHIYKQRKK
                     INLASERQSHRPFVSVGKTDR"
     gene            complement(5947..>6861)
                     /locus_tag="VE170_00330"
     CDS             complement(5947..>6861)
                     /locus_tag="VE170_00330"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ13904.1"
                     /translation="EPVRAYQELYKEGRMSARVSILPRVIESMIESKSLTELGLITGF
                     GNEWLRIGGVKMSIDGGITGRNACFHEPYENDEHNHGIIRIQQDELNHTVQVCHDAGL
                     RCCVHAIGDKAFDMALDAYESAIEHSPRKDHRHRIEHMGNWLATPKLIGRMVRSGIVA
                     VPNIAFAYYIGDAILDCVGERRLTKAFPFHTLLKNGVIMSGGSDSPGYWPVDPLRDIA
                     TAASRKMRWGEVWIPEEKITVAEAFAMHTTTAAWVGFEENDKGTLEAGKLADIAVLAE
                     DPFGVPPEKIRELKVEMTLVGGEVKYQA"
BASE COUNT         1558 a         1876 c         1985 g         1442 t
ORIGIN      
        1 gaaggagcgc gatttcgtca ctgcggctaa ggccttgggc gcccgcgacg cttacgtcct
       61 atttcaccac gtggttccca ataccctggc gagcgtcacg gtaatcgcca cttttggcat
      121 ggccatcgtc atcatcaccg aagcgagcct gagcttccta ggcctcggtg tgccggtgca
      181 tattccgacc tggggctcga tgctcgcgga gggacgcagt tacatcaatc gtgcgccctg
      241 gttgacgatc tttcctggct tggcgatttt catcacggta ttcggcatca atcttttggg
      301 cgatggcttg cgcgatattt tcgatcccaa gctgcggcga cagagataac cggcatcgat
      361 aaagacacag gagggactct atgcttaaga aacgatccgc gcttagtcgg cggcaatttc
      421 tcacacggag cgccgcgatg gccgtgggag cggcagttgg gccgtatttc ttgcgcaatg
      481 cgggtgccgc ttcacgcgac cgggtgacca tctttcattc gagcgtggcc gattcgattc
      541 atccttataa ccatagctcg agtccgattt acggcaattg gcagcatgtc atcgagcctt
      601 tgatcgagta cgatgtcggg cgcaaggatt tcatcggcgt gcttgccgag tcgtggcaat
      661 tccagggtaa caagtgggtt ttcaagctgc gcaagggcgt caagttccac aacggcgcga
      721 cctttacgtc caaggatgtc gtcttttccg tcgacaagat gcgcgacgaa aagggcggca
      781 gcttgcaagc gcccaacttc aaggatgtca gcgagattca gacgccggac gaccttaccg
      841 tggtgtttgt caccaagcag cccaacgcgg tgttcctgga tcggctcgac aaccgcttta
      901 tgttgagtaa ggaggcgggc gataagtttg gcgacaagat ttatgagaat ctcgtcggca
      961 ccgggcccta taaatttgtc agctaccaac gtggcggcaa tatggttttc acccgcaacg
     1021 acgattactg gggcggcaag gcggcgatca aagaagttgt tttcaagaaa gtcaccgagg
     1081 atgccgcgcg cttggcggcg ttggagtcgg gccaagcgga tttcatcaat aacgtgcccg
     1141 atcatgaagt ggcgcgactg aaaaatcatc cgcgcgtgcg catcgatcaa gtcgaagggc
     1201 tgcggatgtt tttcctggcg attaatttcg ccttcaaacc ttgggataat aaactggtgc
     1261 gccaggcggc caactacagc gtcgacgcgc cggcgatcgt tcgcaatatc ttcgacggta
     1321 tcggttatcc ctgcaacggt ccggtgggct ccaccgttct cggggtcgac cccaaacata
     1381 agcgttaccc atacaatccg cagaaggcca aggaactgct gacccaggcg ggctttccca
     1441 acggctgcga cattcagctg tattattcat caggacgtta tccgaaagac cgcgaagtct
     1501 gccaggtgat cgcggcgcag atggtcaagg gcggctttcg cgtcgaactg atttctcagg
     1561 aatgggcgct tttctgggac aagcaggggg tcaacggcgg ccggttgccg ttctattaca
     1621 ttggccgcgg cagcttggcc gatgccgaca cgctctacga tcaatacttt cgcaccggca
     1681 cgaccaagcg caccaactac agcaatgccg aactcgacaa gctgatcgag gaacagcagc
     1741 gcacaccgga caccaaaaaa cgcgtgcaga tcttacagcg ggcgggtaaa ttcatcatgg
     1801 acgaggcgct gtttatcccg ctctacaatc tcgctgatat ctacggggtg gtaaggaatc
     1861 tcgattggaa gaagcgcgcc gatgaaaaag tcaaggcgtg ggacatgaag atcaagtagc
     1921 attgcttgcc agtaagatcg acaatatttt tgcgacggca atgggggagc gatgagcaac
     1981 ggaaaaatca atcgacgaga atttctcaaa cacagcggag tagctggttg cgccgcactg
     2041 ctgggcggcg ccgtcgtcaa actgagctat gccgcgagcc gcgaacgctt gacggctttg
     2101 agcagcatcg gtctcgatac cctccatccc tacgcccata gctcgagccc ccagtatggt
     2161 atctggcata atatgatcga accgttggtg gaagtcgatt acgccaagcg cacttactat
     2221 ggcgttctcg ccgagtcctg ggagtttcag ggcaagcgct gggcgatcaa gctgcgcaag
     2281 aacatccgtt tccatgacag cagcccgttt accgccaagg atgtcgtcta ctcgatcaac
     2341 cggatgaaaa acgacaagct gagcctgcag agggaaaatt tccgcgactt taccgaggtc
     2401 caggcggccg acgattacac cgtcgtcatt accaccgagc agccggtggc ggtgatgctc
     2461 gaccgcttgc agaaccgatt cattctcagc aagaccggca tggaagccca gggtgatcag
     2521 gcagagctga aaccgattgg caccggtcca tacaaatttc aaagctggca gcgcgacggt
     2581 aacctggtga tggtgcgcaa cgatagctac tggggcgcta aaccgtcgat ccgcgaaata
     2641 gttttgcgcc gcgtgcgcga ggactcggga cgtgttgccg gcctcttggc cggccagggc
     2701 gatgtcacca acaacgtgcc ggtggaagag ctgtcgcgct tcgacaatca cccgcgcatt
     2761 cgcgccgaga aggtcgaagg cgtgcgcatg tatttcctcg cgatgaacgt gacgcacaag
     2821 cctttcgata ataaattagt ccgccaggcg attaattacg cggtcgatcc ggcggtgatc
     2881 atcaaacata tttacgaagg caacggctac atcatgaacg ggccgatggg gtccaacgtg
     2941 atcggcttcg atcccaagat caaacgttat ccgctggatc tcaataaggc taaggagctg
     3001 ttgacaaaag ccggttatcc caacggcctc gatgtgaagc tgtatttttc gccggatcgg
     3061 tatccgaaag caaaagaggt gtgccaagtg atcgccgacc agttgtccaa ggtcggcatc
     3121 aaggccgagt tggtgtccca agagtttgtc gtcttctggg gcaaggaagg cgtcaacggc
     3181 ggcaaactgg gtttctacta cgtcggtcgt ccggcagccg acgcggatac ggtctacgac
     3241 cagtactatc gttccggcgt gagcccgcgc atacaataca aaaatccgga gttcgacaag
     3301 ctggtcgacg aagagcaaaa aaccggcgat gccaaaaagc gcctcgccat gctgcatcag
     3361 ctgggccgca tcctgatgga agacgtgccg ttctgcccgc tgtacacgct ggcggaaatc
     3421 tatggcgtgg cgcgcaacat caactggaag gctcggccgg acgaaaaatt actctatgcg
     3481 gagatgaaga ttcgttaacc atgccgacgt ctcggaacga ggagaagcaa cgatgaagag
     3541 aacttcaaat gatatcgatc gccgccgatt tctaaagtat ggcgccctcg ccggcgtcgg
     3601 cgcattggcc ggagcggctt ggcccggttc aagctggtcg gcgtcgaagg agcgcttgac
     3661 gattctcagc agcatcgccc tcgataattt acatccctac gcttacagct ccgggccgca
     3721 gtacggcatc tggtacaaca tgatcgagcc cctagtcgat gtcgattttg ccaagcgtag
     3781 ttactttggc gtgctcgccg agtcctggga gtttcaggga aaaaaatgga tcttcaaact
     3841 gcgcaagaat gtccgcttcc atgacggcag ccggtttacc gccgccgacg tgatctattc
     3901 atttaaccgc gtgaaaaacg acaggctaag cctgcaaaaa gaaaactttc gcgatctagt
     3961 tgagatgcag gcgctcgacg atcatacgct ggtctttgtc accgaagtgc ccaacgccgt
     4021 gtttctcgac cggctagaga atcgtttcat catcagcaaa agcgccaccg aagcgcaagg
     4081 cggcgagccc ggcgagcaca agccggtggg caccggacct tatcgattcg tcagttggca
     4141 gcgcgaaggt aatctggtga tgacgcgtca tgataattat tggggcacga aacccgccgt
     4201 caaagagatc gtcattcgcc gggtgcggga agacgctggc cgggttgccg gattgctcgc
     4261 cggccaaggc gacgtgatca acaacgtgcc ggtggaggaa ctgtcgcgct tcgataaaca
     4321 tgcaaaagtc agagcggaaa aggtcgaagg ccagcgcatg tatttcctgg ccatgaacgt
     4381 gacgcacaag ccgttcgaca acaagttggt ccgccaagcg atcaactacg cggtcgatgc
     4441 gacgacgatc gtcaagcata tctacgaagg caacggctat gtcatgaatg gacccatggg
     4501 ttcgaaggtg atcggcttcg atcccaacat caaacgctat ccggtagacg ttaagaaggg
     4561 taaggagctg ctcgacaagg cgggacatcc caacggtgtg gatgtgaaac tatacttttc
     4621 gccggatcgc tatcccaaag ccaaggaagt ctgccaggtg atcgccgacc agctggctaa
     4681 gatcggcgtc agggttgaac tggtgtcgca ggaattcgcg gtgttttggg gcaaggagtg
     4741 ggtcaatggc ggcaagtcgc ctttttatta cgtcggccgc ccagccaccg acgccgacac
     4801 cgtctacgat cagtattatc gctcgggtgt cagcccgcgc attcaatata aaaatcccga
     4861 gttcgataag attatcgacg aagagcaacg caccggcgac gctaagaagc gcatcgcgct
     4921 gctgcatcaa gccgggcgca tcctgatgga agacgttccc ttcgtgccgc tgtacacctt
     4981 ggcggagatt tatggcaccg cgcgcaatgt cggctggaac gccaggccgg acgagaagat
     5041 cctcgccggc ggcatgaaga tcttatagtt acttgcgccc ctggcggcac gatgggttac
     5101 gctggtccgg gcaggcaagt gaaatttggt acattgtcgt aagttctgta cgcgagggtt
     5161 cacgctcgct tgatctgtca ccgggttaac tctgcacttc gacaaccaat agtcgtcaaa
     5221 agtttgtaaa actttcgcaa ctttccttgg catgcccgtt gctccctata gctgccatgg
     5281 caacagggat tcttaatgcg gtaccgccgg cggcggcaag gatgcgctat gcttcgaccc
     5341 tgcgctgttt cggccagttt ctcgaaggca cggagctcaa ggcggtcgaa atcaaaaccc
     5401 atggcgacga cttcatcctc caggcatgga acaaggggca atcaatggcg atggaccttg
     5461 acaagctttt cacgcctctg gacgtgcaaa agctcgaagc cgacggccac gccaagcgca
     5521 agccgtttgc cggtccgccc aacatgttga gtctgcccca ggtactacgg ctggccggca
     5581 actacgtcga tcgccagcat ggacggttgc tgcgggtgtc gtggcaggac cagtccgaca
     5641 agatccagtc gataacggta cagtgggaag caagcccggc agctagagaa tcaagcgacg
     5701 cacaattgtc ggtgatcgaa gagttgtgca ttcacatcta caagcagcgc aagaaaatta
     5761 atttggcatc cgaacggcaa agccaccggc cctttgtcag cgtcggcaag acggatcgtt
     5821 aaaagttact agcgcactga ttcgcgtgct aaaacaaagg gcgaccctgc ggtcgccctt
     5881 tgttttagtt caacccctcg cccgtctggc tacggcaatg gccgggttgg gttcaccgta
     5941 ggctctttag gcctgatact tcacttcacc gccgaccaac gtcatttcga ctttcagttc
     6001 gcgaatcttt tcgggtggta cgccgaaagg gtcttcggcc aacaccgcga tatcggcgag
     6061 tttgccggct tctagcgtcc ctttgtcgtt ttcctcgaag ccgacccatg ccgctgtcgt
     6121 cgtatgcatg gcaaaggctt cggcaacggt aatcttttct tcgggaatcc acacttcacc
     6181 ccagcgcatt ttgcgcgaag cagcggtggc aatgtcgcgc aaggggtcca ctggccagta
     6241 acccggcgag tcggaaccgc cggacatgat gacgccgttt ttcagcagcg tatgaaaggg
     6301 aaaggccttg gtcaggcgcc gttcgccgac gcaatcgaga atcgcatcgc cgatgtaata
     6361 ggcgaaggcg atatttggca cggcgacgat gcctgagcgg accatgcgac caatcagttt
     6421 cggagtcgcg agccagttgc ccatatgttc gatgcgatgg cgatgatcct tgcgcggcga
     6481 atgctcgatg gcgctttcat aggcgtcgag ggccatgtcg aaggctttgt cgccgatggc
     6541 atggacgcag cagcgcaggc cggcgtcatg gcagacttgc acggtgtgat tcaattcatc
     6601 ctgctggatt ctgataatgc catgattatg ctcgtcgttt tcatagggct cgtggaagca
     6661 ggcgttgcga ccggtgatgc cgccgtcgat gctcatcttg acgccgccga tgcgcagcca
     6721 ctcgttgccg aagccggtga tgagaccgag ctcggtcaag cttttacttt cgatcatgga
     6781 ttcgataacc cgcggtagga tgctgacgcg cgcgctcatg cgcccttcct tgtagagttc
     6841 ctggtaggcg cgcaccggtt c
//
LOCUS       DASQKS010000015         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_428499, whole genome shotgun sequence.
ACCESSION   DASQKS010000015 DASQKS010000000
VERSION     DASQKS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428499"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VE170_00335"
     CDS             <1..754
                     /locus_tag="VE170_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13905.1"
                     /translation="DGLTIGHFIGGLFLQQILGKPGIEFDARRFEYIGAPAQDNFIMG
                     LHKNTGVTSLPQWIASKTVVKFGGAGFGAGTDDLTALARDVMGLPIQLVAGYKGTADI
                     RLAFNNGEILGITNSWQSTRSTWRNELDSGLLHILFQLATKSHPELTKYPLPGDLIKT
                     DDDRKLFQAVAQVHGASVRPFVLPPNTPKERVQLLRKGFTDTLADADFVTEAKKGNIE
                     INPVNGEDLAKSVQSVLQLEPGLVARLKEILK"
     gene            complement(892..1971)
                     /locus_tag="VE170_00340"
     CDS             complement(892..1971)
                     /locus_tag="VE170_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HYJ13906.1"
                     /translation="MATPSVRREAKYVALTKQQFRERFFARFYDPAFDTVKSELEKVF
                     EKAWDGYIDYRKSPRKKPAGRGFADGKFELPIEWLDTRANIHAAEKRQKDAKSLSRVL
                     LINGSTRSEHSCPGEISKTRRLAQQAQKTIESVAGFEVDFLDISTLSDEPYKTIFPCK
                     ACVSTAQPLCHWPCSCYPNHAMGQVNDWMNEIYPRWVAAHGVMILCPVHWYHAPASLK
                     LMIDRLVCADGGNPDPTTTKGKNPLLAKQLELEGWSYPKHLAGRAFSVIAHGDAAGPE
                     NLRRMLTDWLTDMEMIPAGPTALIDTLVGWYQPYATSHDELDKDKALFAEVSNAALAL
                     ANMVKLIRTGKYHAPNEHLHDPRKK"
     gene            2327..3202
                     /locus_tag="VE170_00345"
     CDS             2327..3202
                     /locus_tag="VE170_00345"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ13907.1"
                     /translation="MNQKSQTDLRITGDHRLADEWELVLLAQGLSPSVRQTSDGVVVS
                     VPEAEVGRALVSLSAYDWENPQKPLERVEPIGSGSWVSGIAVGLTLGLFYSITVQWLP
                     ALSWFARGAADAERILQGELWRTVTALTLHADVAHVLSNAIAVGLFLSAVNSMLGAGI
                     GGALVLLAGAGGNLVNAMLHGSPHVSVGASTAVFGAVGLLGSLGLSRRRRNALSGWRA
                     WLPVAAALALLGMLGSSGQRVDIWAHLFGLLAGAVIGLPLGLVKPRATELRLQWTCGM
                     VSLALLVYCWILAFR"
     gene            complement(3436..>3634)
                     /locus_tag="VE170_00350"
     CDS             complement(3436..>3634)
                     /locus_tag="VE170_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13908.1"
                     /translation="SVPANYKVLSDTKYHKVVADLEAILLDISTDGVGINTPDGKISA
                     WTLKSWAQSLRNALETLSKAQ"
BASE COUNT          734 a         1003 c         1070 g          827 t
ORIGIN      
        1 tgacggtttg accatcggtc acttcatcgg tggcctgttc ttgcagcaga tcctcggcaa
       61 gccggggatc gaattcgacg cccgcaggtt cgaatatatt ggcgcgccgg cccaggacaa
      121 tttcatcatg ggtttacaca agaacaccgg cgtcaccagt ctaccgcagt ggattgcgtc
      181 aaaaaccgtg gtcaagttcg gcggcgccgg ttttggagcg ggaacggacg atctcactgc
      241 gctagcgcgg gatgtcatgg gtctgccgat ccaattggtt gccgggtata aaggcaccgc
      301 ggatatccgt ttggcattca acaacggcga aattctcggt atcaccaact cctggcagtc
      361 gacccgctcg acctggcgca acgaactcga tagcggcctg ctgcatatac tttttcaact
      421 ggcgaccaag tcccacccgg agctgacgaa atatccgttg cccggcgacc tgatcaaaac
      481 cgacgacgat cgcaagctct tccaagccgt ggctcaagtc cacggcgctt cggtccggcc
      541 ttttgtcttg ccgcccaaca caccaaaaga gcgcgtgcaa ctcctgcgca agggatttac
      601 agacactctc gcggacgccg actttgtcac cgaagctaaa aaaggcaaca tcgaaatcaa
      661 tccggtcaat ggcgaagatc tggcgaagag cgtgcagagc gtgttgcagc tcgagccggg
      721 attggtggcg cggttgaagg aaatcctgaa ataattccgg caccatcgag cacggacgag
      781 ctagatgacg ccgtgcggag catcaagacc gtttagaagg tacatccatc tcgaagtcgt
      841 tccgtttcca ggactccccg ctcaatctga tctaatccta gaggccgctg tctatttctt
      901 tcggggatcg tgcaagtgct cgttcggcgc gtgatacttg cccgtgcgaa tgagtttcac
      961 catgttggcg agcgccagcg cggcgttgct cacttcggcg aaaagggcct tatctttgtc
     1021 gagttcgtca tggctggtgg cgtagggttg gtaccagccg accaaggtgt cgatcagcgc
     1081 cgtcggaccc gcgggaatca tttccatatc cgtgagccag tcggtcaaca tgcggcggag
     1141 attttccggg cccgcggcat cgccatgggc gatcaccgag aacgcccggc cggcgagatg
     1201 cttggggtag ctccagcctt cgagttccag ctgcttcgcc aatagcggat tttttccctt
     1261 cgttgtcgtc ggatcaggat tgccgccgtc agcgcagacc agacgatcaa tcataagttt
     1321 taaactcgcc ggcgcatgat accaatgcac ggggcaaagg atcatcacac cgtgggcggc
     1381 cacccagcgc ggataaatct cattcatcca gtcgttgacc tggcccatgg cgtgattggg
     1441 ataacaagag catggccaat gacagagtgg ctgcgcggtt gaaacgcacg ccttacaggg
     1501 gaaaatggtt ttgtacggtt cgtcggagag cgtcgagatg tcgagaaagt cgacttcgaa
     1561 gccggctaca gattcgatgg tcttttgcgc ctgctgcgcc aaacggcggg ttttggaaat
     1621 ctcgcccgga cagctgtgct cgctgcgggt cgagccgttg atgagcagga ctcgagacaa
     1681 tgacttggca tctttctgac gcttctcggc cgcgtgaatg ttggcgcggg tgtcgagcca
     1741 ttcgatcggc agctcgaatt tcccatcggc gaatcctcga cccgccggtt tcttacgcgg
     1801 tgacttgcga taatcgatgt agccgtccca agccttctcg aaaactttct ccagctccga
     1861 ttttaccgta tcgaaggcag ggtcgtagaa gcgcgcaaag aaacgctcgc gaaactgctg
     1921 cttggtcaaa gccacatact ttgcctcgcg ccggacgctt ggggtagcca cgacgatcct
     1981 ccgttagaaa cttgatggga gtcagagcgg cttggttggc agaattgata agcgcatcaa
     2041 gccatttcaa gcgaactcct gacttcaaga tctgtatatc gctagtaggc aagttagtcg
     2101 atgacgatat ccgccgggca tggtccgagc ctggccgcaa tttatcgacc agcggtacag
     2161 gcatctcagc ttactcttgc cgctggaccc cgagttcact gcaacaaaga gccaaacgtt
     2221 tgttcgcggc tggtcatcca cagtgataag taaatagcat tggacatctg ccgcacatga
     2281 ggtcgcacgc tgcctcgaac aatttgctga ttttgccagc cagtcaatga accaaaaatc
     2341 gcaaaccgac ctgcgcatca ctggcgatca ccgattagct gacgagtggg agcttgtgct
     2401 cctggctcaa gggctatcac cgagcgtacg ccaaacatcg gacggcgtcg ttgtcagcgt
     2461 gccggaagca gaggtggggc gagcgctggt cagtctttcg gcttacgatt gggagaaccc
     2521 gcaaaaacca ctggagcgag tcgagccaat cgggtcgggg agctgggtta gtggcattgc
     2581 ggtggggctg acgcttggat tattttattc catcacggtt caatggctgc cggcattgtc
     2641 ttggtttgcg cgcggtgccg ccgacgccga gcggatactt caaggtgaac tttggcggac
     2701 ggtgaccgcg ctcacgctcc atgccgatgt cgcccatgta ttgagcaacg ctatcgccgt
     2761 tgggctattt ctgagcgcgg ttaacagcat gttgggtgcg ggtatcggtg gcgctctcgt
     2821 tctgctggcc ggcgccggtg gcaatcttgt caatgccatg ttgcacggtt cgccgcacgt
     2881 ttcggtcggt gcctccacgg cggtttttgg cgcggtcggt ttgctcggta gcctcgggct
     2941 gagtcggcgg cgccggaacg ctttaagcgg gtggcgcgct tggctgccgg tggccgcggc
     3001 gcttgccttg ctcggaatgc tcggctctag cggtcagcgc gttgacatct gggctcatct
     3061 cttcggtctg ctggctggcg ccgtaatcgg gttacccctg ggcttagtta agccgcgtgc
     3121 aaccgaactt cgactccaat ggacctgtgg catggtgtct ctcgccctgc tcgtttattg
     3181 ctggatactc gcgttccggt aatggcggat tgccggaact gatttgcaat cttaccggcg
     3241 cgcttgcctt ggactacttg gtaatggcga tcacaggacg cggcgatgcc acggcaacgt
     3301 cgatattttg acggatctcc agacagtgaa ggttccctgt gcaagatgaa tacgccaccg
     3361 gctgccgtcg aggcgggcta tcagccagta gcagagatgg ttgggagtcc gataatttct
     3421 caaaagcgcg ctggtttact gggccttgga taaggtctcc agcgcatttc tcagagactg
     3481 ggcccaactt ttgagggtcc acgcgctaat tttaccgtcc ggggtgttaa tgccgacgcc
     3541 gtcggtgctg atatccaaca aaatggcttc caagtcggca accaccttat ggtatttagt
     3601 atcggataat actttgtagt ttgccggcac cgac
//
LOCUS       DASQKS010000016         6582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_849032, whole genome shotgun sequence.
ACCESSION   DASQKS010000016 DASQKS010000000
VERSION     DASQKS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849032"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VE170_00355"
     CDS             complement(<1..380)
                     /locus_tag="VE170_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13909.1"
                     /translation="MATLSRNYRIVLITLAACQLLIGGTVFGQTAPFYKDKTIRIIVG
                     FTPGGLYDQYARLLARHMGKYIPGNPTIIVQNMPGAGSLSATNYIYGVAKPDGLTLGM
                     IGSGIYLDQLLGRPEARFDVGKFA"
     gene            679..906
                     /gene="tcuB"
                     /locus_tag="VE170_00360"
                     /pseudo
     CDS             679..906
                     /gene="tcuB"
                     /locus_tag="VE170_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456145.1"
                     /note="with CitA (TcuA) catalyzes the oxidation of
                     tricarballylate to cis-aconitate; originally thought to be
                     involved in citrate utilization; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization protein TcuB"
     gene            903..1604
                     /locus_tag="VE170_00365"
     CDS             903..1604
                     /locus_tag="VE170_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HYJ13910.1"
                     /translation="MKALPEIVRIPEGFFLMGCETGQDNERPVHRVWLDCFGLAKFPV
                     THAEYDLFLDATGGAPPRFWSEPMFADPRNPVVGVTWDEAVAYCRWLGGREGLQYRLP
                     TEAEWERAARGGRDGALYPWGDEAPSMRTAIGFNAATGGPAPVGVNPPNDFGLHDMSE
                     GVHEWCSDYYDYNYYSYSPEHNPQGAVSGERRVSRGGSWRHQIKFSRSAARSSLPPSF
                     CYADYGFRVAMTITR"
     gene            1795..2781
                     /locus_tag="VE170_00370"
     CDS             1795..2781
                     /locus_tag="VE170_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13911.1"
                     /translation="MTRWKITYGFFICSVVSLFIVTPSRAIDNIRVACPSLSSTSVFA
                     LVVAQKEGYFREEGLNVELLSIRGEIAIRTVLAGDVDFFTNAGSALAAGVRGIPVRIV
                     TVFQDKPSWDLIAQPEVKSIAQLRGKNIAVMSPEGSLAVVAREMLRKNGLDPTKDVNL
                     VTMGGDEVRFPALQTRSIQATLFNTAMSIKAQKDGFIKLGNASEYANLIEGGLATSQD
                     KIQQQPEKIHRFVRGALKGLIFYVSKREAAVKYMMETLRMKDQELVSSIYDILTPLAL
                     RAGFSADKVLQTMIDEMKRTTKVQRDIKAPDIFDLSFVKKANDEIKASGWKP"
     gene            complement(2875..3846)
                     /locus_tag="VE170_00375"
     CDS             complement(2875..3846)
                     /locus_tag="VE170_00375"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13912.1"
                     /translation="MIRSIAKFILIAMCGFVAASAPALGADKVHMSSTNITAFDLDQI
                     VALAKGFFAKENIEFEPTYMQPDLVIKALLAGTVDLAKSGTHFGMIAATRGGELKVIG
                     GSTYGYAYQIIGQPQFKTIADLKGQKIAGAGVASITTTIFKDVMQRQGIPPSAYTILS
                     IGGSAERFQAVASGQVAASLAEAPPYNFRSIDSGKRVLLNYSDEIKSIQYGSYFASNK
                     SLAQMRPVLMRFMRAIGQSMRWLNDPVNEKEAVRIMMDRLKIDETIAARTFKFMVPEN
                     HSFRGESMVDPVGLNEMIRLLAMDNLIPERKPWETFVDAGFLPVAAK"
     gene            complement(3876..4964)
                     /locus_tag="VE170_00380"
     CDS             complement(3876..4964)
                     /locus_tag="VE170_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007260895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYJ13913.1"
                     /translation="MKITNVKASIHRVPVEVPLLKEKIVTPIVFTSVETDMGVTGYGL
                     TRGGQRFGMKEFINREVAPFLRGKNPLETERVWNDLLKQFNARVQTGMWSSAVSTIDI
                     ALWDIKGKHYKEPVWRLLGGAQNPVQAYVTFGLKQYKKEQLVEVAKQLIAQGEKRLKM
                     VVAVDPENPKVDAERVKAVCKAVGDDVEIMIDANYLFSYNRALELCKMVEPLNITWFE
                     EPVYQNDALLLADLRRHTSIPIAAGQNEGHRFRHRELIANRAVDIAQPNVCSVGGYTE
                     AVKVAALAQAFNLPIANGGGWPHHNMHLQAAMANGWRVEFHFEMWGVGDKIYQEPPAP
                     VNGWVTLAETPGLGLEPRFDALDEFEEK"
     gene            5158..6105
                     /locus_tag="VE170_00385"
     CDS             5158..6105
                     /locus_tag="VE170_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13914.1"
                     /translation="MWRTLVTSVIMICGSFTVAHAQPVRGAFPSPSVQILPMMVAQER
                     GFFKREGLDLELIFVRGASTAVQALIANQIHFIFSIGPQMPAVWEGHDIMLLAQQVGR
                     PTFSLVVTPDIHKIADLKGKKIGVTFGGSTAAGTKALLELNKLNPDKDVEYIGLPGNE
                     PKIAAMKQGIISAALLAPPADYLAMKSGLKRLVSLADIFKDTAFTGLAAHSKTIKENP
                     QMVKRMVRAIVRSVLHTRDNPEDALQVLIKRLGLERDAAQDAYQMIKDALVPVPTEKG
                     VELMAQWQAIALNIKPKRKPAEYMDLRFVNEAMAELAQK"
     gene            6315..>6582
                     /locus_tag="VE170_00390"
     CDS             6315..>6582
                     /locus_tag="VE170_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13915.1"
                     /translation="MKTIKSLAQVWVIVLLAGCAAFSTGSQVQSGRRALLVNDPELAL
                     PYFLQAAQADPNYIYQSVNFREGIWTYVGRTQYATKRYAEARQSL"
BASE COUNT         1364 a         1770 c         1974 g         1474 t
ORIGIN      
        1 cacgcgaatt tccccacgtc gaacctggcc tccgggcggc cgagcagttg atcgagatat
       61 atgccgctgc cgatcatgcc gagggtgagg ccgtccggtt tggcaacgcc gtagatataa
      121 ttggtcgcgc tgagcgatcc cgcgcccggc atgttttgca cgatgatggt cggattgccg
      181 ggaatatatt tacccatgtg gcgcgccagc aagcgcgcgt attgatcgta caggccaccg
      241 ggagtgaagc caacgatgat gcgaatagtc ttatctttgt agaaaggggc ggtctggccg
      301 aagacagtcc cgccgattaa caactgacac gctgctaggg tgatcagtac aatgcgatag
      361 ttgcggctta gagtcgccat gtaatgctcc tttgttcggt tcagcaacag tttataggga
      421 gtttgccgag cttctgcaaa tccgcaagaa agtatcatga gatgtgtatt gcgatatttt
      481 tccgatttgg cttataaatg tcgatcatta cacgtgcgtg aggaatctat gcgaataata
      541 agttggctgt caggctgcgc taggcgaacc gagtggatcg cctgcttgtg cttggttctg
      601 atcgccgctc tatttcgtcc cggctttggg caagaggcat cgaagaagct tctggtcgcc
      661 tactcgggac tgatcaacat ggacgtggcg ttcttgctgc tgttgtttct caccagtttt
      721 accggactcc tactgccggt gttcagagag accatggcga tgggaacgtt attggtcatt
      781 catctcagcg tggtagccgg acttttcatc acgatgccgt acggcaaatt cgcccatata
      841 gtttaccgct atgcggcctt ggtgcgattt tccatcgaac agcgccgcgt cgaaggcaac
      901 ggttgaaggc acttcctgaa atcgtccgca tccctgaagg attttttctg atgggctgtg
      961 aaaccggtca ggacaacgag cggccggttc atcgggtgtg gctggattgc tttggtttgg
     1021 caaagtttcc agtcacccac gcggagtatg atttatttct cgacgccacc ggtggcgcgc
     1081 cgccgcgatt ttggtccgaa ccaatgtttg ccgatccgcg gaacccggtg gtaggcgtga
     1141 cctgggacga agcggttgcg tattgccgct ggctcggtgg ccgcgaaggg ctgcagtatc
     1201 gtttgccgac cgaagctgaa tgggaacgcg ctgcgcgtgg cgggcgagac ggtgcgcttt
     1261 atccttgggg tgatgaagcg cccagcatgc gcacggctat tggcttcaat gcggccacgg
     1321 gtggcccggc accagtcggc gttaacccgc ccaacgactt cgggcttcac gacatgagcg
     1381 aaggcgtaca cgagtggtgc agcgactatt acgactacaa ctactatagc tactctcccg
     1441 agcacaatcc ccagggcgcg gtttcaggcg agcgccgggt ttcacgcggc ggctcgtggc
     1501 ggcaccagat caagtttagc cgctcggccg cgcgcagttc gctgccgccg tcgttttgtt
     1561 acgccgatta tggttttcgc gtggcaatga caattacacg atagcttgcc gacgcgttcg
     1621 aagagttccc gccatgcaag cgcggcttgc atggcgaaga gtccggggcg ggcgggctgt
     1681 tttctgccga aatgttttgt ttgaccgaag tcatcgataa agataaatag cgaagaccga
     1741 agcgcgcgcg ccgagagcaa cgcaaattag tctggacgag gaaggagctt tagcatgaca
     1801 cgctggaaaa taacctatgg gtttttcatt tgctcggtcg tttctctctt tatcgtcacg
     1861 ccgtcgcgcg ctattgacaa tatccgggtg gcttgtcctt cgctgagtag tacttcggta
     1921 tttgccctgg tcgttgcgca aaaggaaggt tactttcgag aagaaggtct taacgtcgag
     1981 ctgctcagca tccgcggcga gatcgcgatc cgcacggtgc tggccgggga cgtcgatttt
     2041 tttaccaatg ccggcagcgc gttggcggcg ggggtcagag gcattcccgt caggattgtt
     2101 acggtttttc aggataagcc cagttgggat ttgatcgcgc aacccgaggt caagtcgata
     2161 gcccagttgc gcggtaagaa catcgcggtg atgtctccgg aagggtcgct tgcggtggtc
     2221 gcgcgcgaaa tgttgcgtaa aaacggcttg gacccaacga aggatgtcaa tcttgtgacg
     2281 atgggaggtg acgaggtccg ctttccagcg ctccagaccc gatccataca ggcgacgttg
     2341 ttcaacacgg ccatgagcat caaggcgcaa aaggatggct ttatcaagct cggaaacgcg
     2401 agcgagtacg ccaacttgat cgaaggcgga ttggcgactt cgcaagacaa gatccagcaa
     2461 cagccggaaa aaatccatcg cttcgtccgc ggcgcgctga aagggctgat attttacgtc
     2521 agcaaacggg aagcggcggt caaatacatg atggagacgt tgcgcatgaa ggaccaggag
     2581 ttggtcagtt cgatctatga cattctcacg ccgctcgcgc tccgggcggg attcagcgcc
     2641 gacaaggtgc tgcaaaccat gatcgacgag atgaaacgaa ccaccaaagt acagcgcgac
     2701 atcaaagccc ccgacatttt cgatctgagc ttcgtaaaaa aagccaacga cgagatcaaa
     2761 gcgagcggct ggaagccgtg acgctgagcc gccgctagca ggttgttcaa atcgttcaaa
     2821 tagctcaagc cgttcaaaac gtacgaatcg gaggccgacg actttctcgc cagatcattt
     2881 tgccgcgacc gggagaaatc ctgcgtcgac gaatgtctcc cagggtttgc gttcggggat
     2941 tagattatcc atcgcgagca ggcggatcat ttcattcaat ccaaccgggt cgaccatgct
     3001 ttcgccgcgg aaggagtggt tttccgggac catgaatttg aacgtccgcg cggcgatggt
     3061 ttcgtcgatc ttcaagcggt ccatcataat ccgcaccgct tccttttcgt tgaccggatc
     3121 gttcagccag cgcatcgact ggccgatggc gcgcatgaag cgcatcagca ccggacgcat
     3181 ctgcgccagc gatttgttcg aggcaaagta ggagccgtat tggatgctct taatctcgtc
     3241 ggaatagttg agcagcaccc gtttgccaga gtcgatggag cgaaagttat agggcggcgc
     3301 ttcggcgagg gatgctgcga cttgaccgga cgccaccgct tgaaaccttt ccgcgctgcc
     3361 gccgatggat aggatcgtgt atgccgacgg cgggatgccc tggcgctgca tgacgtcttt
     3421 gaagatcgtc gtcgtgatgc tggccacgcc ggcgccggcg attttctggc ctttcagatc
     3481 ggcaatagtc ttgaactgtg gctggccgat gatttgatag gcgtagccgt aggtcgaccc
     3541 gccgatgact ttcaactcgc cgccgcgcgt cgccgcgatc ataccgaagt gggtgccgga
     3601 tttcgccaag tcgacggtgc cggcgagcaa agccttgatc accaggtctg gctgcatgta
     3661 ggtcggctcg aattcgatat tctccttggc gaagaaacct ttcgctagag caacgatctg
     3721 atcgaggtcg aaggccgtga tattggtcga actcatgtgg actttgtcgg cgccgagagc
     3781 gggcgccgag gcggcgacaa agccgcacat cgcgattaag atgaacttag ctatggatcg
     3841 tatcattttt cgtcccctgt ttagccatgc ccttttcact tctcctcgaa ctcatcgagc
     3901 gcatcgaagc gcggttcgag tcccagtccc ggcgtctccg ccaaggtcac ccagccgttc
     3961 accggcgccg ggggttcctg gtagatcttg tcgccgacac cccacatctc gaagtgaaac
     4021 tcgacgcgcc agccgttggc catggcggct tgcagatgca tgttatgatg cggccagccg
     4081 ccgccgttgg cgatcggcag gttgaaggct tgcgccagcg cggcgacttt taccgcttcg
     4141 gtgtagccgc cgaccgagca gacattgggc tgggcgatat cgacggcgcg gttggcgatt
     4201 agttctctat ggcgaaaacg gtgcccttcg ttctggccgg cggcaatggg aatcgacgta
     4261 tggcgccgca agtcggcgag caacagggcg tcgttttgat ataccggctc ttcgaaccaa
     4321 gtaatgttca ggggctcgac catcttgcac aattccaggg cgcgattgta agaaaataaa
     4381 taattggcat cgatcattat ttcgacgtcg tcgccgacgg ctttgcacac cgctttgacc
     4441 cgctcggcgt cgactttggg attctccgga tcgacggcga cgaccatttt caaacgcttc
     4501 tcgccttggg cgatcagctg cttggcgacc tcgaccagct gctctttctt gtattgtttt
     4561 aacccgaagg tgacataggc ctgcaccggg ttttgcgcgc cgccgagcag gcgccagacc
     4621 ggttctttgt aatgcttgcc cttgatatcc cacagggcga tatcgatggt gctgaccgcc
     4681 gagctccaca tgccggtttg cacgcgggcg ttaaattgtt tcaacaagtc attccagact
     4741 cgctcggtct ccagcgggtt cttgccgcgc aagaacggcg cgacttcacg gttgatgaat
     4801 tccttcatgc cgaaccgctg tccgccgcga gtcagaccgt agccggtgac gcccatgtcg
     4861 gtttcgacgc tggtgaaaac gatcggtgtg acgatttttt ccttgagcag cggcacctcc
     4921 accggcaccc ggtggatcga tgcttttaca ttggtgattt tcaagcaata cctccagatt
     4981 cggctgacag ttatcctgga acggggcgga actgcaagta gaaaggatta gaattaagca
     5041 gcgataggtt atgccatttc gagcgcagcg cgatcaccat cgagccattg gcaaatagtt
     5101 cttgacggcc gctcgtaagc gctttaagag aacaaagcac tgttggaggg caatcctatg
     5161 tggcgaacat tggttacttc ggtgatcatg atctgcggca gtttcaccgt ggctcatgcc
     5221 cagccggtgc gcggcgcgtt tccatcgccg agcgtgcaga tcctgccgat gatggtggcc
     5281 caggagcgcg gcttttttaa gcgcgagggg ttggatcttg aactgatttt tgtccgcggc
     5341 gcgtcgaccg cggtgcaggc gctgatcgcc aatcagatcc attttatttt ttccatcggg
     5401 ccgcaaatgc cggcggtgtg ggagggccac gatatcatgc tgctcgccca gcaggtcggc
     5461 cggccgacat tttcactggt ggtgacgccc gatattcaca agatcgccga tttgaagggg
     5521 aagaagatcg gcgtcacctt cggcggctcg acggcggcgg gaaccaaagc gttgctcgaa
     5581 ctcaacaagc tcaatcccga caaagatgta gagtatatcg gcttgccggg caacgagccc
     5641 aagatcgcgg cgatgaagca gggaatcatc tcggcggcgt tgctggcgcc gccggcggat
     5701 tatttggcga tgaaatcggg gctcaagcgg ctggtgagct tggcggatat tttcaaagac
     5761 acggcgttca ccggcctggc ggcacatagc aagactatta aagagaaccc gcagatggtt
     5821 aaacgcatgg tccgcgccat cgtccgctcg gtgctccaca cccgcgacaa cccggaagat
     5881 gcgctgcaag tcttgatcaa gcgcctcggc ctcgagcgcg acgcggcgca ggatgcctac
     5941 cagatgatca aagacgcgct ggtgccggtg ccgacggaaa agggcgtgga gctgatggcg
     6001 cagtggcaag cgatcgcatt gaacatcaaa ccgaagcgca agccggcgga gtacatggac
     6061 ctgcgcttcg tcaatgaagc gatggccgaa ctggcgcaga agtgaatgaa gcgccggtca
     6121 gttgaagtag catagtctca tgtaatgtat gggattctga ggcgatcgaa cgaatggccg
     6181 ttattacggc ggaggcagaa atcgaggcga ggctgtgtcg cgccggcttt gtgcatggcc
     6241 gctgggcttt ccgatggctt agaccgccgc gccgtcattg acattggttg tcgagggacc
     6301 tagtttttga tcgtatgaag acaataaaaa gcctcgcgca ggtctgggtg attgttctcc
     6361 tcgccggctg tgccgctttc agcaccggga gccaggttca atccggccgg cgtgccttgt
     6421 tggtgaacga tcccgagctc gcgctgcctt actttctcca agcggcgcag gccgatccca
     6481 actacatcta tcaatcggtc aattttcgcg aaggtatctg gacctacgtc ggacgtacgc
     6541 aatacgcaac caagcgctat gccgaagcgc gccagtcact gg
//
LOCUS       DASQKS010000017         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2949220, whole genome shotgun sequence.
ACCESSION   DASQKS010000017 DASQKS010000000
VERSION     DASQKS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2949220"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1468
                     /locus_tag="VE170_00395"
     CDS             278..1468
                     /locus_tag="VE170_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ13916.1"
                     /translation="MTSAAPTLTAMPTAAAKDTAKIAVSLRGLWKTYTGSSKPAVQNL
                     SLDIHDGEILTLLGPSGCGKTTTLRMVAGLEHPDAGDILFGEQAVVMTANRLFMPPDK
                     RNVGMVFQSYAIWPHMTVAENVAFPLHARHFPKSEIKGRVLKALDLVGLAGFEDRPGP
                     LLSGGQQQRVAFARALVTEPRVLLLDEPFSNLDAKLREQMRIGVKLLQKRLNIAMLFV
                     THDQIEALSLSNRIALMNFGVVQQEGDPHLLYEKPANEFVRDFVGRTLLFKGKVQSGN
                     PAGQLAIAIAGAPDCVVFGRTYNPDGIKTGAAVYVGVRPEDVEILPASGTTAPPGMIG
                     GVAQAALFIGERIEYQVEVPGQGELLVYGQRHQPVDEGNKVWLKLRADGHSAWPSDWS
                     LKND"
     gene            1486..2661
                     /locus_tag="VE170_00400"
     CDS             1486..2661
                     /locus_tag="VE170_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ13917.1"
                     /translation="MNTLRKPVTQYLPRWFGSVLFFFLGVGTAFGAAATANFELEWPK
                     LITAAQQEGTLSIASGGAPSRQYRPVVDAFSKKFNIKVEVSTGNATDTVNRVLAERKA
                     GKHTVDVALISSRENQQRLVPSDSLVPIGPLLFHPDVIDMSKWYRGRHMYADKFSKFT
                     LIYHAGLEDQYEIWYNTDKIKEAEIATLVKQTDVFDPRWKGKIAGQGMGDPSGIRQMI
                     DSYFEPDRGPEWVRRYMVNTGVTFSDDRRILETWLVNGRFPLQFVATGTEEYVALARK
                     GLPIKARFLVKKAGVLRATGSGCCISAFASAPHANAAKLFINWFLTKEGQTLTHTLIP
                     NLDRSSLRTDVPFGEVSPQQRRGYGKEYAFPDADPAMGEKQEEVQKWVFKLWESRQK"
     gene            2777..>4110
                     /locus_tag="VE170_00405"
     CDS             2777..>4110
                     /locus_tag="VE170_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ13918.1"
                     /translation="METSPTIDQLAMPRALPAPRLTRGHFLMAVVLVSLGYFLIWPVL
                     LLLINSFNAASDWFVEPRRWGLTHWFNAFQRPGLLRSLMNSLLIWSLTVGISFPIGVG
                     IAWLLARTKIPCSRTLEFLFWVSYMVPSLPTTIAWITLLDPDIGMLNVGLKNLFGLEQ
                     GPFNIFSVPGIVWANLMGHGISIKVMLMTPAFRNMDATLEEAARVGGASNLRTLFKVT
                     LPLMVSPMIMVFALQLLRVFQSFETEYLLGVPFGFYVYSTKVYSLVRSAVPNYGEATV
                     LASITLLMIVLIIPLQRWILERRRYTTITGSFRPGLIDLGKGNFIAFAFLATLLSLLT
                     FGPMLILVLGSFMQRIGYFVLGFTLEHWRLVLSDPVFVKALRTTLTLALTAAVLSPLI
                     FSVLAYLLVRTQLPGRATLDLMIWGAGAIPGILAGLGLLWVFIGTPGLNALFG"
BASE COUNT          842 a         1276 c         1110 g          882 t
ORIGIN      
        1 cccgcctgga gctggcacca tcatgagaac agcaacggcg acgacacgat ccttttctcc
       61 atcgacgact ggccggcgat gcaaaagctt ggcttctata tgaaggcgga agcccagggg
      121 ttctgaaagc cggcggagtg caacctgtcg gctcgatctg gaatttagtc gccagccatc
      181 aattgtcccg ccgtttgatt cgcagcggcg aacattaatc cgcccgccga atccccccta
      241 gagtaccgcg caactttcgt gtagaagaat cagcaccatg acgagtgccg cgccaacttt
      301 aaccgccatg ccaacggcag cggccaaaga tactgccaaa atcgccgtca gcctgcgcgg
      361 cctgtggaaa acctacaccg gcagcagcaa accggcagta caaaatctct ccctcgacat
      421 ccacgacggc gagatcctca ccctgctcgg ccccagcggc tgcggcaaga ccaccacgtt
      481 aagaatggtc gccggcttgg aacatcccga cgcgggcgac attctcttcg gcgaacaggc
      541 cgtggtcatg accgctaatc ggctattcat gccgcctgac aagcgcaatg tcggcatggt
      601 gtttcagtcc tacgccatct ggccgcatat gacggtggcc gaaaacgtcg cgtttccgct
      661 ccacgcgcgc cactttccca agagcgaaat caaaggacgc gtgttgaaag ccctcgacct
      721 ggtcggcttg gccggctttg aagatcggcc ggggccgctc ctgagcggcg gccagcaaca
      781 gcgtgtcgcc ttcgcccgcg cactggtcac cgaaccgcgc gtgctgctgc tcgacgagcc
      841 gttcagcaac ctcgacgcga aattgcgcga acagatgcgc atcggcgtca agctgctgca
      901 aaaaagactg aacatcgcca tgctgttcgt cacccatgat cagatcgaag cgctcagtct
      961 atccaatcgt attgcgttga tgaatttcgg cgtggtccag caagaaggcg atccccattt
     1021 actgtatgaa aagccggcca atgaattcgt ccgcgacttc gtcggccgca cgctgctctt
     1081 caaaggcaaa gtgcagagcg gcaacccagc gggccaactg gccatcgcca tcgcaggggc
     1141 gccggactgc gtggtcttcg gccggaccta caatccagac ggcataaaaa ccggcgccgc
     1201 tgtctatgtc ggcgtacgtc ccgaagatgt cgagatccta ccggcgagcg ggaccacagc
     1261 gccaccaggc atgatcggcg gagtcgccca agcagcgctg ttcatcggcg agcgcatcga
     1321 ataccaggta gaagttcccg gccaaggcga gttattggtt tacggccaac gacatcaacc
     1381 cgttgacgag ggcaacaaag tctggcttaa gctgcgcgcc gatggccata gcgcctggcc
     1441 gtccgactgg tcgcttaaaa acgattgagc aatatggaga actaaatgaa cactcttcgc
     1501 aaaccagtaa cccagtatct gccgcgatgg ttcggatccg tcttattctt tttcctcggc
     1561 gtaggcaccg ctttcggtgc cgccgcgacg gccaatttcg agctagaatg gcccaaactc
     1621 atcaccgcgg cacagcagga aggcacgctt tcgatcgcgt ccggcggcgc gccgtcgcgc
     1681 caatatcgtc cggtggtcga cgccttcagc aagaaattca acatcaaggt cgaggtctcc
     1741 accggcaacg ccaccgacac cgtcaaccgc gtcttggccg agcgcaaagc cggcaaacac
     1801 actgttgatg tggcgttgat cagctcccgc gaaaatcagc aacgcttggt gccatccgac
     1861 tcgttggtac cgattggccc gctgctgttt catcccgacg tcatcgacat gtcgaagtgg
     1921 taccgcgggc gccacatgta cgccgataag ttcagcaagt tcactctgat ctaccacgcc
     1981 ggtttagaag accagtatga gatctggtac aacaccgaca aaatcaaaga agcggagatc
     2041 gctacgctgg tcaaacagac cgacgtcttc gatcccagat ggaaaggtaa aatcgccggc
     2101 caaggcatgg gcgacccgtc cggaattcgc caaatgatcg acagctattt cgagccggat
     2161 cgcggccccg aatgggtaag gagatacatg gtcaacaccg gcgtcacctt cagcgacgac
     2221 cggcgcattc tcgaaacctg gctcgtaaat gggcgctttc cgctgcaatt tgtcgccacc
     2281 ggcaccgagg agtatgtcgc cctcgcgcgc aaaggcttgc cgatcaaggc gcgcttcctg
     2341 gtcaagaaag cgggcgtcct gcgcgcgact ggttcgggct gctgcatctc agcatttgcc
     2401 agcgcacccc atgccaacgc cgccaaatta tttatcaatt ggtttttgac caaagaaggc
     2461 cagactctaa cgcacacgtt gatccccaat ctcgatcgct cctcgttgcg caccgacgtc
     2521 ccgtttgggg aagtgtcgcc gcagcagcgc cgcggttacg gtaaagagta cgcctttccc
     2581 gacgccgacc ccgccatggg cgaaaaacaa gaggaagtgc agaagtgggt ttttaagctg
     2641 tgggagagtc ggcagaagta aggttcaaaa cgttccaatc gttcaagccg ttcaaatcgt
     2701 tttcgcatcg ctcggttcga acctccacac caacggcttc aatgatttga acagcttgaa
     2761 tctccccagc taaaccatgg aaacatcgcc aaccatcgat cagttagcca tgccgcgggc
     2821 gctgcccgcg ccccgtctca cccgcggcca cttcctgatg gcggtggtgc tcgtttctct
     2881 tggatatttt cttatatggc cggtgctcct gctgctgatc aacagcttca atgccgccag
     2941 cgattggttt gtcgagccgc gccgctgggg ccttacccac tggttcaatg cctttcagcg
     3001 ccctggactg cttcgttcgc taatgaattc gctgctcatc tggtcgttga cggtgggcat
     3061 cagttttccc atcggagtgg gcattgcctg gctgctcgcc cgcaccaaga ttccatgcag
     3121 ccggacgttg gaattcctat tctgggtttc ctatatggtg cccagcttgc cgacgaccat
     3181 cgcctggatc acattgctcg accccgatat tggcatgctc aacgtcggct tgaaaaatct
     3241 cttcggtttg gagcagggac cattcaatat ttttagcgtg cccggcatcg tctgggccaa
     3301 cctcatgggc catggcatct cgatcaaagt catgctcatg acgcccgcct ttcgcaacat
     3361 ggacgcgacc ctggaagagg cggcccgggt cggcggcgcg agcaatttac gaactctgtt
     3421 caaagttacg ttgccgctga tggtctcgcc gatgatcatg gtgttcgcgc tgcagttgct
     3481 gcgggttttt cagtccttcg agaccgagta cttgctcggc gtaccgtttg gcttctacgt
     3541 ttattcgacc aaagtttatt ctctggtgcg cagcgctgtc cccaactacg gcgaagccac
     3601 cgtgctcgcg agcatcaccc tcttgatgat cgttttgatc attccactgc agcgctggat
     3661 cctggagcgg cgccgttaca cgaccatcac cggcagtttt cgccccggcc tcatcgatct
     3721 cggcaagggc aatttcattg cctttgcttt cttggccacg ctcttgtccc tcttgacctt
     3781 cggccccatg ttgattctcg tactcggcag tttcatgcag cgcatcggct actttgtttt
     3841 gggctttacc cttgaacatt ggcgcttggt gctctccgac ccggtgttcg tgaaagcgct
     3901 gcgcacgact ctgaccttgg cactgacggc ggcggtcttg agcccgctga tattttccgt
     3961 gctcgcctac ctgctcgtgc gcacacagct ccccggacgc gcgaccttgg atctgatgat
     4021 atggggcgct ggcgcgatac ccggtatcct cgccggcctt gggcttcttt gggtgtttat
     4081 cggcacgcct ggtttgaacg cgctcttcgg
//
LOCUS       DASQKS010000018         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2950130, whole genome shotgun sequence.
ACCESSION   DASQKS010000018 DASQKS010000000
VERSION     DASQKS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950130"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VE170_00410"
     CDS             <1..1280
                     /locus_tag="VE170_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ13919.1"
                     /translation="IQMRIDHHKLFEPLTKATLACRHANVGAAFAQAIAVATQEPPGP
                     VHLDLPEDVAQAAAVENRGAPAASAPLPDLSNEVAATIRHALQRSKRPLLIGGLSFTR
                     CAAGGNLLQFVERQNIPFVTTLHGKGFLPESHANSAGVIGRARRSDVRKFTDRADLII
                     AVGYDPIEINYEDWVTSGTPIVHVSTEAAENGNDLNLLWNKACNLDAAVGAISHISAA
                     ANDWSISDFSAHRDVWDKALRPASANFAPFQALDILRAALPADGILAYDVGAHTHQIA
                     SQWRTDLARTLLATNGWSSMGYGMPAAYAAKLVHPDRAVVCVVGDGGFQMTAGELALA
                     RRLNLAVPVIVLNDGWLGLMKVKQEKRNYELSGVRLGAPPESPAHYFGVPCRAAKTPD
                     EFRDALEWAMALGGPSVIEAFVDAASYSTTVFD"
     gene            1295..2515
                     /locus_tag="VE170_00415"
     CDS             1295..2515
                     /locus_tag="VE170_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ13920.1"
                     /translation="MPADSSVKRHPGRWMILALISVSHIIGAAAQYGVNTLAPFYKDE
                     LGLSRTQVGLFFSSFYLAMTGASFGTGWLADRFGVRKTTLLGHVLLGLCTMMAAFSHS
                     FEFGCVSFFLAGFGYSFLNPASTIGVMSWFHRDERATAMGAKQTGVPGGGVLAAIVAP
                     SLVLLIGWRGSLAALGVLNVIFGFIFSWLWREPPAGTAVAETNPGVTSTDGPLNVWGL
                     LPISCATAMYLIAQMALITYVPLYLKDAMEFSPYWASQALALTQAGAMIGRIGWGVVS
                     DRIFKGRRKIVLILIGILAMILFTGLSLMGPNSSYFILMAILFLSGVCIVGYQGVSYA
                     LIGEITGKAKTGAGLGMMITINAGAATLGTPIFGYIVDRTGSYSIGWQVLAAAIAIGT
                     FAMAILLNDTARKY"
BASE COUNT          483 a          797 c          818 g          620 t
ORIGIN      
        1 gtattcagat gcgcatcgat catcacaaac tattcgagcc gctgaccaag gcgaccttgg
       61 cttgccgcca cgccaatgtc ggcgcagcct tcgctcaggc gattgccgtc gcgacgcaag
      121 aaccgcccgg gccggtgcat ctcgacttac cggaggatgt cgcccaggcc gccgccgtgg
      181 aaaaccgcgg cgcaccggcg gcgtccgcgc cgctgccgga tctatcgaac gaagttgccg
      241 cgacgatccg ccacgcgctg cagcgttcca agcggccgtt gttgattggc ggtttgtcgt
      301 tcacccgctg cgcggcgggt gggaatttac tacagtttgt cgaacggcag aatattccat
      361 tcgtcacgac cttgcatggc aaggggtttt tgccggagag ccatgcaaac tcggccggcg
      421 tcatcggccg ggcgcgccgc agcgatgtca gaaagttcac cgatcgcgct gatctcatca
      481 ttgccgttgg ctacgatccc atcgagatca actacgaaga ctgggtgact tccggcacgc
      541 cgatcgttca cgtcagcacc gaggcggctg agaacggcaa cgatttgaat ctgctctgga
      601 ataaggcgtg caacttggac gcggcagttg gcgctatcag ccacatttcg gccgcggcca
      661 atgactggtc gataagcgat ttctcggcgc accgcgatgt ttgggacaaa gccctccggc
      721 cagccagcgc gaactttgcg ccgtttcagg cattggatat tttacgcgca gcactgccgg
      781 ccgacggcat actggcttac gacgtcggcg cccacaccca tcaaatcgca tcccagtggc
      841 gcaccgatct ggccaggact ctgcttgcca ccaacggatg gtcgtcgatg ggctatggca
      901 tgccggcggc ctacgcggcg aaattggttc atcccgatcg cgcggttgtt tgcgtggtcg
      961 gcgacggcgg cttccaaatg accgccggtg aattggcgct ggcgcggcgg ctaaacctag
     1021 cagtaccggt gattgtgctc aacgatggct ggcttggtct gatgaaggtc aaacaggaaa
     1081 aacgcaacta tgagctttcc ggcgtgcgtc tcggcgcgcc gccggaatca ccggcgcatt
     1141 atttcggcgt cccttgccgc gccgctaaga cgccggatga atttcgtgat gccctcgagt
     1201 gggctatggc ccttggcggt ccgagcgtca ttgaagcttt tgtcgacgcc gccagctata
     1261 gcacaacggt cttcgactaa cgtccatcca ttccatgcct gccgacagct cagtaaagcg
     1321 ccatcctggc cgctggatga tcctcgcgtt gatcagcgtg tcgcatatca ttggcgccgc
     1381 ggcgcagtat ggcgtgaaca ctttggcgcc gttctacaag gatgaactcg gattatcgcg
     1441 cacccaggtg ggattgtttt tctcgtcatt ctatttagcc atgaccggtg cctcgtttgg
     1501 caccggttgg ctggcggatc gcttcggcgt gcgcaaaacc acgctgctcg gccatgtgct
     1561 cctgggtcta tgcaccatga tggcggcatt ttcgcattcc ttcgagtttg gctgcgtcag
     1621 ctttttcttg gccggatttg gctacagctt tctcaatccg gcgtcgacca tcggtgtgat
     1681 gtcgtggttt caccgcgacg agcgcgccac ggccatgggc gccaagcaga ccggcgtgcc
     1741 ggggggcggt gtgcttgcgg caatcgtcgc gccgtcgctg gtgttattga tcggttggcg
     1801 tggctcgtta gcagctttgg gcgtgctcaa tgtcattttc ggcttcatct tctcatggct
     1861 gtggcgcgaa cctcccgccg gtaccgcggt cgccgaaacc aaccctggcg ttacaagcac
     1921 cgacggcccg ctcaatgttt gggggctatt gccgattagt tgtgcgacgg cgatgtattt
     1981 gattgcccag atggcattga tcacttatgt gccgttgtat ctgaaagacg ccatggagtt
     2041 ttcgccttac tgggcaagcc aggcgctcgc cctcacccaa gcgggggcga tgatcggacg
     2101 gatcggttgg ggcgtggtga gcgaccgcat cttcaagggc cgccgtaaga tcgtcctgat
     2161 cttgatcggc atactcgcga tgattctgtt tactgggtta agtttgatgg gaccaaactc
     2221 gtcctatttt atcctcatgg caatcttgtt cctctcaggt gtctgcattg tcggttacca
     2281 gggcgtttcc tacgcgctca tcggcgagat taccggtaag gccaagaccg gcgccggcct
     2341 cggcatgatg atcacgataa atgccggcgc ggcgacgttg ggcacgccga ttttcggtta
     2401 catcgtcgat cgcaccggct cctattcgat cgggtggcaa gtcctcgcag cggcaatcgc
     2461 catcggcacg ttcgctatgg cgatcctgct caatgacact gcgcgcaagt attagcacgg
     2521 aggggggtca aggcggttgc tcgaatcgat gccggcggta ttcaattcat ctcagctcgt
     2581 catgccgagg ctggacggca gcgtcgtgag gtcggcctgc acattcttga tgccgggaat
     2641 cgctttgatg tgggtgagga cttgccgttc ggtaatccat tccggcacag ttccactgac
     2701 aaaaatatcg ccaccatt
//
LOCUS       DASQKS010000019         9674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4210233, whole genome shotgun sequence.
ACCESSION   DASQKS010000019 DASQKS010000000
VERSION     DASQKS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4210233"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3219)
                     /locus_tag="VE170_00420"
     CDS             complement(<1..3219)
                     /locus_tag="VE170_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2644..2646),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYJ13921.1"
                     /translation="MEVSRRDFLRLSVAAGTGTALSGLIGSGVNLGPAVARAQELRIK
                     NAKVTPSICPYCSVGCGTLVHTINGQIVNIEGDPRSPHNEGTLCPKGAAIFQLHVNPN
                     RPTQVLHRNAGATDWEVWDLERAMNRIAELVKKTRDETFIEKLPNGKLVNMTPAIFAL
                     GGATLEVEFNHVHQKLMRGLGIVAIENQARIUHSSSVPGLGTRFGRGAATLYPRSMYD
                     SDCIIVMGSNMAENHPVAFRWPMKAKVEHGAKIIHVDPRFTRTSAVADIYAPVRAGSD
                     IAFLGGIIKYVLDSEKWNTDPFFKKFVVNYTNAATIINEGFKDTEDLDGVFSGLMQSK
                     GGVAEWPFNGITNQYDGATWQYASTKVGAQGEMAAATAQSGETKVTTGPDGTKAAAKI
                     ELKPGEGFAPLVASLVRPAPMKDETLQHPRCVFQIVKRHFARYTPEMVENATGCPKET
                     FLQVAETILANSGAERTTSFAYAVAWTQHTYGVQIIGACALLQLLLGNIGRPGAGVMA
                     LRGHASIQGSTDIPTLYHSIQGYLAAPTALKNHDTLQDYIAAETIPTGYWANMPKFMI
                     SYLKSVYGDAATKDNQFGYDWHPKILGDHSHMAMFAAMDAGRVRGMICIGQNPATSIN
                     AKLERSGLRKLEWLVVKDNWLTETATFWRNAPEIKNGEVKTADIKTEVFFLPATQVAE
                     LEGTFTNTQRLLQFHHKAAEAPGDCRSDTLFSYDLGKRLKKLYADSTAARDEGFKNLV
                     WDFEHENEAERKKGEPSALKILKEINGFFTDDPARHCVGFGDLKDDGSTTCASWIYSG
                     VYPAVGKLLADRRTPDPPGKPGAQLEWGWAWPANRRVMYNRASADPEGKPWSEKKKWV
                     WWDGEAKKWVGYDVPDYIAAKPPTDKAKPGAAGMDALPGTAPFIMRPDGLGWLFVPSG
                     LVDGPLPTHYEPVESPVANSLYKQQTSPVYKHWQADGNVLAKMGDPKFPYVLTTYRLT
                     EHYLAGGMSRWLPWLAELQPELFVEISPELAKEKGIKNLDWCKISSPRTQIRAKALVT
                     RRMRPFKIDGKVIHQVGMPWHWGYEGLSTGDVVNELTSLIADP"
     gene            complement(3426..4517)
                     /locus_tag="VE170_00425"
     CDS             complement(3426..4517)
                     /locus_tag="VE170_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ13922.1"
                     /translation="MAEALLTLRDILVRYGPTTILEFSSLSVHAGENLAVIGPNGAGK
                     STLLRLMAQLQQPTQGKIYFHDREALPSNGLAMRRRMASVFQEPLLLNASVYDNAALG
                     LRLRGLDRRSIEGRVQPWLERLGIEHLRRRRARSLSGGEAQRTSLARALALDPEMLFL
                     DEPFSPLDPPTHEALLLDLQRILRETGITTVFVTHDRDEAFMFGDRTAVLIGGKLLQT
                     GPTMEVFAHPENLEVAQFIGFDTKIPGVVESANEQSAQVRFNGGYAQVFGDFQAGEEL
                     FLCLRPEDIAVSLPGVEGPKSSAGNQLRGKALKVTPWGAHYRVAAACGENRLVALITK
                     PAFLGRKIAEGDEIVAAFEASAVHVIKRN"
     gene            complement(4499..5212)
                     /locus_tag="VE170_00430"
     CDS             complement(4499..5212)
                     /locus_tag="VE170_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ13923.1"
                     /translation="MELIWEGIKHAGLLLLSRDAEVLGITLFSLQVSSAATGLSLVVG
                     IPLGMLLAMKRFPGHSLAVSLINTGMGLPPVVVGLFVTIFLWRSGPLGFMELLYTPTA
                     MIIAQVIIAFPIVAGLTMAAFQSLNPNLPLQLLGIGASTPQLLWLLCKEARLPLLAAI
                     MAGFGGVISEVGASMMVGGNIRGQTRVLTTATVLETGKGNFEIAIALGLILLVLTFAV
                     NLLLTHIQQREQIRWPKPS"
     gene            complement(5214..6083)
                     /locus_tag="VE170_00435"
     CDS             complement(5214..6083)
                     /locus_tag="VE170_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYJ13924.1"
                     /translation="MPYKLSFPIVLAVLALVLAVPSNLPAGEAASKNVILSTTTSTQD
                     SGLLDVLVPLFEKKSGYSVKTVSVGTGQALELAAKGEADVALVHAPSLEKKYVADGKL
                     LNRRLVMYNDFILLGPENDPAQIKSTKSSPKSSIDAMKRIAASANRFVSRGDNSGTHN
                     LEKSLWKQAGIEPKGAWYIESGQGMGATLGIANDRNGYTITDRATYLAFRKRVTLAIL
                     LEGDRPLLNIYSVMEVNPANGPRINAAGGKIFADFMVAPETQAVIKSFGVEKFGQPLF
                     IPVAGMREEQVGE"
     gene            complement(6099..6998)
                     /locus_tag="VE170_00440"
     CDS             complement(6099..6998)
                     /locus_tag="VE170_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ13925.1"
                     /translation="MNEFFNTKELAKYIGINEKKVYFMAKAGQLPCTRVTGKWVFPKR
                     LIDEWIEENSRGRTSSKKTEQREFLLAAGSDDPSLGILRELYTDQKTAASIFFATSGS
                     TDGLVALRDGVADFALSHLHDPETGEYNLPFVRTTIPSGAALVPLFHRELGLLLGAGN
                     PLGVRTLADLGRTGVRMINRQIGSGTRHYTDQQFSQLGIDTKRIEGYGMAVATHLEVG
                     LKILRAQADVGIASGTAAQMLGLDFIPLTRERFDIVIPKARFFSPGIQRLLEIVGSRD
                     FRGRVEALGGYDTSDSGRMIASN"
     gene            complement(7179..7358)
                     /locus_tag="VE170_00445"
     CDS             complement(7179..7358)
                     /locus_tag="VE170_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13926.1"
                     /translation="MPTDETRRVLKVFGVAVTAFEDAVDKEAAPEELEKADAELKLRL
                     AQVTAFIERLRARLH"
     gene            complement(7382..9229)
                     /locus_tag="VE170_00450"
     CDS             complement(7382..9229)
                     /locus_tag="VE170_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HYJ13927.1"
                     /translation="MHGFHGRLLHIDLTCGQISWRALEESRLRAFLGGIGLGTSLLYE
                     FAPPGVDPYAPANPLIFTSAPLVGTGLTTTAKFAVVTKSPLTGFIADSLSSSHFALEL
                     KRVGVDALVITGQASSMVYVLIENDRVEIRPADDLRGHSPSETESMIRSQLSSRATRV
                     AAIGQAGEKLVRFATISNEGRHAGRGGVGAVMGSKHLKAIALSGDREISVADPSGVEA
                     IAGSLRRRSLGSLTDKYRSIGTVANLAVFNRLGSLPTRNFQQSTFDQAEALSGETLTV
                     NNFSRRHGCASCTIQCERLFKALDGGEQRLEYETLFALGPLCGIKDPEVVLRAAQLCD
                     LYGLDTISTGGTLSWAMECSEKGLLPDARSLRFGEANALLDAIPMIAQRQGLGALLAE
                     GSRRAALALGNDSSYWAMHVKGLELPGYEPRSLKTMALGLTVSPRGACHNRSGAYEAD
                     FSGAVDRLESDSGRGSIVAASEDYAAVLDSLIVCKFLRKCFSDFYLEGAALLGKVTGW
                     DYSSDELKHTGERIHNLKKLFNQREGWRTEDDWLPQRLLSEVLPTGVAQGVGLSADDL
                     REMIAGYYQARGWDENGFIRQSKLTELGIEAPESSLEFPANRETRNSNL"
     gene            complement(9233..>9674)
                     /locus_tag="VE170_00455"
     CDS             complement(9233..>9674)
                     /locus_tag="VE170_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYJ13928.1"
                     /translation="DKVCTRKVTESEMHPHDPESLSFLNMNTPEDYQAALTSWNEGRR
                     AEIDAAPTQSTPVTVELFGVARLLANTKEVSLSLPQAATLSQVCQALALEHPVLLGRV
                     INPDRSSLSSGYACNINGLEFVKNPNARINPGDKVFILSADAGG"
BASE COUNT         1910 a         2771 c         2928 g         2065 t
ORIGIN      
        1 cggatcggct atcagtgacg ttagctcatt gacgacgtct cccgtcgata ggccttcata
       61 gccccagtgc cagggcatgc ccacctggtg aatgactttg ccgtcgattt tgaaggggcg
      121 catgcggcgc gtaaccagcg ccttggcgcg gatctgggtg cgcgggctgg agatcttgca
      181 ccagtcgagg ttcttgatgc ctttttcctt ggccagctcg ggactgatct cgacgaaaag
      241 ctcgggctgc agctcagcga gccagggcag ccatcggctc attccgcccg ccagatagtg
      301 ctcggtcaac cgataggtgg tcagaacata gggaaacttg gggtcgccca tcttcgccaa
      361 gacattgccg tcagcttgcc aatgtttgta taccgggctg gtctgctgtt tgtagagcga
      421 attggcgacc ggcgattcca ccggctcgta gtgggtcggc agcggcccgt ccaccagtcc
      481 cgaaggcacg aacagccatc ccaatccgtc cggccgcatg ataaatggcg ccgtgccggg
      541 gagcgcgtcc atgcccgcag cgccgggttt cgctttatcg gtaggcggct tggccgcgat
      601 atagtccggc acgtcgtagc cgacccattt cttcgcctcg ccgtcccacc agacccactt
      661 tttcttctcg ctccacggct tgccctcggg gtcggcagag gcccggttgt acatcacccg
      721 ccggttcgcc ggccatgccc atccccactc gagttgcgcg ccgggtttgc cgggcgggtc
      781 gggtgtgcgt ctgtcggcga ggagcttacc gacggccgga tagacgcccg agtagatcca
      841 cgacgcgcag gtcgtcgaac catcgtcctt gagatcgccg aaaccgacgc agtggcgcgc
      901 cggatcgtcg gtaaaaaagc cgttgatctc ctttaaaatc tttaaggccg aaggctcacc
      961 ttttttgcgc tcggcttcgt tctcgtgctc gaaatcccag acgagatttt tgaagccctc
     1021 gtcgcgcgcc gcggtcgagt cggcgtaaag ctttttcagc cgcttgccga gatcgtaact
     1081 gaacaatgtg tccgatcggc aatccccggg cgcctcggcc gctttatgat ggaactgcag
     1141 caggcgctgg gtgttggtga aggtgccttc gagctcggcg acctgggtgg ccggcaagaa
     1201 gaaaacttcg gtttttatat ccgctgtctt gacctcgccg ttcttgattt ccggcgcgtt
     1261 cctccagaag gtcgccgtct cggtaagcca gttatccttg acgaccagcc attcgagctt
     1321 tctaaggccg ctccgctcga gttttgcgtt gatcgacgtg gcagggttct ggccgatgca
     1381 gatcatgccc ctcaccctgc cggcatccat cgccgcgaac atggccatgt gggagtggtc
     1441 gccgaggatc ttcggatgcc agtcgtaacc gaactgattg tctttggtcg cggcgtcgcc
     1501 gtacaccgac ttgagataag aaatcataaa cttcggcata ttcgcccaat agccggtcgg
     1561 tattgtctcg gctgcaatgt aatcctgcaa cgtgtcgtga ttctttagcg ccgtcggtgc
     1621 cgccagataa ccctggatcg agtgatagag cgtgggaatg tccgtagacc cctgaattga
     1681 ggcgtggccg cgtagcgcca ttacgcccgc ccccgggcgg ccgatgttgc cgagcaaaag
     1741 ttgtagcagc gcgcaggccc cgatgatctg gacgccgtag gtatgctgcg tccaggcgac
     1801 cgcgtaggca aacgaagtcg tccgttccgc gcccgaatta gccagaatgg tctctgcgac
     1861 ctggagaaaa gtttctttgg ggcagccggt ggcgttctcg accatctccg gcgtgtagcg
     1921 ggcgaaatgg cgcttgacga tttgaaaaac gcagcgcgga tgctgcagcg tctcgtcttt
     1981 catcggcgcc ggccgtacca acgacgctac cagcggcgcg aatccttccc ccggtttgag
     2041 ttcaatcttt gccgccgcct tcgtcccgtc gggtccggtg gtgactttgg tctcgcccga
     2101 ctgcgccgtc gccgcggcca tctctccctg ggcgccgact ttggtgctgg catattgcca
     2161 cgtcgcgccg tcgtattgat ttgttatacc attgaacggc cattcggcga ccccgccttt
     2221 ggattgcata aggccggaga acacaccgtc taaatcttcg gtatccttaa agccctcgtt
     2281 gatgattgtc gctgcattgg tgtagttgac gacaaatttc ttgaagaatg ggtcggtgtt
     2341 ccacttctcg ctatcgagaa cgtatttgat gatgccgccg agaaaggcga tgtccgatcc
     2401 ggcgcggacc ggcgcgtaaa tgtcggcgac ggcgctggtg cgggtgaagc gcgggtcgac
     2461 gtgaatgatc ttggcgccat gctcaacttt ggctttcatc ggccagcgga aggcgacggg
     2521 atggttctcc gccatattgg agcccatgac gatgatgcag tcggagtcgt acatgctgcg
     2581 cgggtagagg gtggccgcgc cgcggccgaa tcgggtcccc agaccgggga cgctagagct
     2641 gtgtcatatc cgcgcttgat tctcgatggc gactatcccg agtccgcgca tgagtttttg
     2701 gtgcacatgg ttgaactcga cctcgagggt ggcgccgccg agggcgaaga ttgcgggcgt
     2761 catgttgacg agtttgccgt tgggcagttt ctcgatgaag gtttcgtcgc gggtcttttt
     2821 caccagctcg gcgatgcggt tcatggcacg ctcgaggtcc catacctccc aatcggtggc
     2881 gccagcatta cgatgaagaa cttgggttgg acgattggga ttgacgtgga gttgaaagat
     2941 cgccgcgccc ttggggcaga gggtgccctc gttgtgcgga ctgcgcggat cgccctcgat
     3001 gttgacgatc tgcccgttga tcgtgtgaac cagtgtgcca cagcccaccg agcaataggg
     3061 gcagatgctg ggagtgacct tggcgttctt gatcctgagt tcctgcgccc gcgcgaccgc
     3121 cggccccaaa ttaacgcccg acccgatcag gccactgagc gctgtgccgg tgcccgcagc
     3181 gacagacagg cggagaaaat ctcgtcgtga aacttccatg ttttgactcc tgtttctgaa
     3241 gcgaaaggat ggagtgaaaa agagcagcca gggggaagcg acatgatttc acaatgggtc
     3301 agccgacaaa tttattgccg gaccaagctc catgggcggc aacggtgaaa cggctggcat
     3361 cctatcgctg ggaaaatcaa tgtcaaggca taatagagtc ggtgtcgagg caatacttcg
     3421 acacttcaat ttcgtttaat cacatgaacc gctgatgctt cgaacgccgc aacgatctcg
     3481 tcgccctctg ctattttacg tccgaggaat gccggtttgg tgataagggc gaccagacgg
     3541 ttttctccgc atgcagccgc cacccggtag tgagcgcccc aaggagtgac tttcagtgct
     3601 ttgccgcgaa gctgattacc agcgctcgat ttgggcccct ccacccccgg cagactcaca
     3661 gcgatatcct ccggtcgaag acaaagaaac agctcttcgc cggcttgaaa atcaccgaac
     3721 acctgggcat agccgccgtt aaagcggact tgggccgatt gttcgtttgc gctctccacg
     3781 actccaggga ttttggtgtc gaagccgatg aactgagcga cctccaggtt ctccggatga
     3841 gcaaagactt ccatcgtcgg cccggtctgc agcagcttgc cgcctatgag caccgcagtt
     3901 cggtcgccga acatgaaagc ctcatcacga tcgtgggtga cgaatacggt ggtgataccc
     3961 gtttcgcgca gtatgcgctg tagatcaagc agcagggcct cgtgcgtggg cggatcaagg
     4021 ggagaaaagg gctcgtctaa aaagagcatc tcgggatcga gcgccagggc gcgagccaag
     4081 ctggtgcgct gggcctcgcc gcccgagagg ctgcgagcgc ggcggcgcct gagatgctcg
     4141 atgccgaggc gctcgagcca gggttgaacc cgcccttcga tggagcggcg gtccaaaccg
     4201 cggagtctta agcccaatgc cgcgttgtca tagaccgagg catttaacag cagcggctct
     4261 tgaaaaacgc tcgccatgcg gcgccgcatc gcgaggccat tggacgggag tgcttcgcgg
     4321 tcatgaaaat agatcttacc ctgggttggc tgctgcagtt gtgccatcag gcggagcaag
     4381 gtcgattttc ccgcgccatt gggtccgatg acggcgagat tttcaccggc gtgaacggac
     4441 agcgaagaaa attcaaggat ggtggttggg ccgtagcgga ccagaatgtc ccgaagagtt
     4501 aggagggctt cggccatcgg atctgctcgc gctgctgaat gtgagtaagg agaagattca
     4561 ctgcaaacgt gagaaccagg agaattaaac cgagggcgat ggcgatttca aagttgcctt
     4621 tgccggtctc caaaactgtc gcggtagtca gtacgcgggt ctgaccgcgg atgtttccgc
     4681 cgaccatcat cgacgcgccg acttcggaaa tgactccgcc aaacccggcc atgatcgccg
     4741 cgagcaaagg cagccgggct tccttgcaga gcagccagag gagttgcggg gtcgatgcgc
     4801 cgattcccag taactgaagc ggcaaattgg ggttgaggct ctggaatgca gccatggtca
     4861 ggccggcgac gatgggaaat gcgattatca cctgagcgat gatcatcgcc gtcggggtgt
     4921 agagcaattc cataaagccg agcgggccgc tgcgccagag aaagatcgtg acgaacaaac
     4981 cgacgaccac gggcgggagt cccatgccgg tattgatgag gctgacggcg aggctgtggc
     5041 ctgggaaacg tttcatggcg agtaacattc ccagcggaat tccgacgacg agggatagtc
     5101 cggtggcggc gctggaaacc tgcaacgaga aaagggtaat gccgagcacc tcggcatcgc
     5161 ggctgagcaa gagcaaaccg gcatgcttga tcccttccca gatgagttcc acgctattct
     5221 cccacctgtt cttcgcgcat tcccgcgacc ggaataaaca gcggctggcc gaacttttcc
     5281 acgccgaagc tcttgatcac ggcctgggtc tccggcgcaa ccatgaaatc ggcaaagatc
     5341 tttccaccgg cggcgttgat tcgcgggccg ttggcgggat tcacttccat caccgaatag
     5401 atgttcaaga gcggcctgtc gccttccagc aggatcgcca gagttacccg cttacgaaag
     5461 gccagatagg tcgcgcggtc ggtgatcgtg tagccgttcc ggtcattagc gatgcctagc
     5521 gtcgccccca ttccttggcc tgactcgatg taccaagcgc ccttgggctc aattcccgct
     5581 tgtttccaaa gcgacttttc aaggttgtgg gtgccggagt tgtctccacg agagacgaat
     5641 ctgttcgcgc tcgccgcgat ccgcttcatc gcgtcaatgc tgctcttggg gctgcttttg
     5701 gtcgatttga tctgggcggg atcgttctcc ggaccgagca agataaagtc gttgtacatg
     5761 acgaggcggc gattgagcag cttgccgtcg gcaacatatt ttttctccag gctcggcgca
     5821 tggaccagcg ccacatctgc ctctcccttg gccgccaact cgagggcttg gccggtgccg
     5881 accgagaccg tctttacgct gtagccgctc tttttctcga acagcggcac cagaacatcc
     5941 agcagccccg agtcctgggt gctggtggtg gtcgacagga tgacgttttt gctggccgct
     6001 tctccggcgg gaagattcga tggaaccgcc agaaccaaag ccaggacagc gagcacgatt
     6061 gggaaagaaa gtttatatgg cataaccact tccgcgattt agtttgacgc gatcatccgg
     6121 ccggagtcag aggtatcata gccgcccaac gcttcgacgc ggccgcggaa atcccgcgat
     6181 ccgacgatct cgagcaagcg ctgaatgccc ggggagaaga atcgcgcttt gggaatcacg
     6241 atatcgaatc gctcccgagt gagaggaatg aagtcgaggc cgagcatctg agcggccgta
     6301 cccgaggcga ttcccacgtc ggcctgcgcg cgtagaattt tcaacccgac ttcgaggtgg
     6361 gtggcgacgg ccatgccgta tccctcgatc cttttcgtat cgatgccgag ttgggaaaat
     6421 tgttgatccg tgtagtgacg cgtgccagag cctatctgcc gattgatcat ccggacaccg
     6481 gttcgtccaa ggtcggcaag cgttcgcaca ccgagcggat ttcccgctcc gagcaatagg
     6541 ccgagttcac ggtggaataa cgggactagc gcggcacctg aaggaatcgt tgtgcggacg
     6601 aagggcaggt tatactcgcc ggtttcagga tcgtgaagat gagatagggc aaagtcagcg
     6661 acgccgtctc ggagcgcgac caatccgtca gtgcttccgg aagtcgcgaa aaagatcgat
     6721 gcggccgtct tttgatccgt gtagagttca cgcagaatcc cgaggctcgg gtcatcgctc
     6781 cccgctgcca gaagaaattc tctttgctcc gtttttttgc tgctggtgcg gccccggcta
     6841 ttttcctcga tccattcatc gatcagccgc tttgggaaaa cccactttcc ggtgacccgg
     6901 gtacagggga gctggccggc cttcgccatg aaatagacct ttttctcatt gatgccgatg
     6961 tactttgcca actccttggt attgaaaaat tcgttcaatt agaccccctg agaagcgcgg
     7021 ccatttgttt attgctgttc gagataaatt gcaactaaat ataacaaaaa ataacttatc
     7081 ctaacaaaaa ctaacccgca aatgggttga aatggctgct ttgtccgcgt tctgaaacac
     7141 caaggagggt aaaactgtgg tcggtggaca gatctggatt aatgcaagcg cgcgcgcagg
     7201 cgctcgatga aggcggtaac ttgtgcgagg cgaagcttca gctcggcgtc agccttttcc
     7261 agctcctcgg gcgccgcctc tttatcgacc gcatcctcga atgcggtcac ggcaacgccg
     7321 aacactttca ggacgcgccg ggtttcatcg gtgggcatac cattcccttt catcatacgg
     7381 gttaaagatt cgagttccga gtttcgcgat tggccggaaa ctccagactc gattccggag
     7441 cttcaatgcc taattccgtt agcttgctct gccgaatgaa cccattctca tcccagcctc
     7501 ttgcttggta atagccggca atcatttcgc ggagatcgtc ggcgctgagg cccacgccct
     7561 gagccacgcc ggtgggaagg acttcgctga gcaaccgctg gggcagccaa tcgtcttcgg
     7621 tgcgccagcc ctcgcgctgg ttgaatagtt tttttaagtt atggatgcgc tcgccggtgt
     7681 gtttgagctc gtcgctggaa taatcccagc cggtgacttt gccgagcagc gcggcaccct
     7741 cgaggtaaaa atcgctgaag cacttgcgca gaaatttgca aacaatcagc gagtcgagca
     7801 cggcagcgta atcctcggat gcggcgacga tcgatccgcg gccggagtcg gattccagac
     7861 gatccaccgc cccggaaaaa tcggcctcgt aggcgcccga gcgattgtgg cacgcgccgc
     7921 gcgggctcac cgtcagacct agggccatgg ttttcaaact gcgcggttca tagccgggaa
     7981 gctccaggcc tttgacgtgc atggcccaat aggaactgtc attgccgagc gcaagcgccg
     8041 cccgccttga gccttcggcc aacagcgcgc ctaagccttg ccgttgagcg atcatcggga
     8101 tggcgtcgag cagagcgttt gcttcgccga agcgcagcga cctcgcgtcc ggcagcaagc
     8161 ctttctcgct gcactccatc gcccaggaca aagtgccgcc agtgctgatt gtgtcgagac
     8221 cgtagagatc gcagagctgc gcggcgcgca ggaccacctc gggatctttg atgccgcaga
     8281 gcgggccgag cgcgaataag gtttcgtatt cgagccgctg ctcgccgccg tcgagagctt
     8341 tgaacaggcg ctcacattgg atcgtacagg atgcgcatcc atgccggcga ctgaagttgt
     8401 tgacggtgag agtttccccg cttagcgcct cagcctggtc aaaggttgat tgctggaaat
     8461 tacgcgtcgg caaagatccc aatcggttga aaaccgctag attggccacg gtgccgatgc
     8521 tgcggtattt gtcggtgagc gagccgagac tgcggcggcg cagggagccg gcgatggcct
     8581 cgacaccgct gggatcggcc actgaaatct cccggtcgcc ggacagagcg atggctttga
     8641 gatgcttcga acccatcacc gcgccgaccc cgccgcgccc ggcgtgacgt ccttcgttgc
     8701 tgatcgtcgc aaagcgcaca agcttttcgc cggcttgacc gatggccgcc acccgcgttg
     8761 ccctagagct cagctgcgag cgaatcatcg attcggtttc gctcgggctg tgtcctctca
     8821 ggtcgtcggc tggtctaatc tcgactcggt cattttcgat caggacgtac accatcgacg
     8881 atgcctggcc ggtgattacc agagcgtcga cgccgacacg tttgagctcg agggcgaagt
     8941 ggctcgacga cagcgagtcg gcgataaagc cggtgagcgg cgacttcgtg accacggcga
     9001 atttcgcagt ggtcgtcagt ccagtgccga ccaacggtgc gctggtgaaa atcagcgggt
     9061 tagccggcgc gtaggggtcg actccaggcg gggcaaattc atagagtagg cttgtgccga
     9121 ggccgatgcc accgaggaaa gcgcgcaggc gggactcttc aagcgcgcgc catgagatct
     9181 ggccgcaggt cagatcgatg tggagcagtc gtccatgaaa gccatgcatg gctcagcctc
     9241 cggcatccgc agaaagaata aagactttat ctcccggatt gatcctggcg ttgggattct
     9301 tgacaaactc cagcccatta atgttgcacg cgtaaccgct cgacaggcta ctccggtctg
     9361 gattgatgac tcggccgagc aggaccgggt gctcgagcgc aagcgcctga cagacttgcg
     9421 acagcgtggc cgcttgcggt aacgacaaag aaacttcttt ggtattcgct aatagccggg
     9481 ccacgccgaa aagctccacg gtgacggggg tggattgagt tggcgcggcg tcgatctcgg
     9541 ctctgcgtcc ttcgttccag cttgttaggg cggcttggta gtcctccggc gtattcatgt
     9601 tgagaaagct caggctctcc ggatcatggg gatgcatttc agattcggta actttgcggg
     9661 tgcagacttt gtca
//
LOCUS       DASQKS010000020         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_10049, whole genome shotgun sequence.
ACCESSION   DASQKS010000020 DASQKS010000000
VERSION     DASQKS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10049"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VE170_00460"
     CDS             <1..586
                     /locus_tag="VE170_00460"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ13929.1"
                     /translation="LWFSDLRNYTRISDSAEPEQIIPLLNEYADAILSAINECGGDIL
                     KLMGDGTLAIFTADARASACHAALAAADTARAAVALVNQRRSADNLPITDMYLGLHIG
                     EVYYGNIGSRERLDFTVVGPAVNEVSRIAGLCRSVDQPLLVSSAFASAIGEPKTLLVS
                     VGRYALRGVGKPQDLYTLDRQREKSAVLDDDSTQ"
     gene            complement(850..1347)
                     /locus_tag="VE170_00465"
     CDS             complement(850..1347)
                     /locus_tag="VE170_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HYJ13930.1"
                     /translation="MHRFGRCWAFFLPVYFLAAAALDPSEVTNGEYLKFVLATRHAAP
                     EYWVNGCFPSGKDAEPVVLVNFHDVSGYCRFVGRRLPTFDEWKSTCEAGKLKKRGDIW
                     EWTSTDVDISGQTYRALCSPANSCDCSHRYLPEWKNQVKGFRCVQDQMPVTWLPEWFR
                     GAVVL"
     gene            complement(1477..>2272)
                     /locus_tag="VE170_00470"
     CDS             complement(1477..>2272)
                     /locus_tag="VE170_00470"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13931.1"
                     /translation="FFREQNLDVKLLLTRTEVDRAALASGNLHYTLRAGSTFVSAARG
                     LPVRIVFLGTTKPFWGLVVRPEIQSVSELKGKSMGVPGLLGSQQISAKFIVKQGGLDP
                     DRDIIYRVVDTGTRIGGMLSGSIDSAMMDYGEAFRAKKMGLKLLINAADYHGLLAGGL
                     GVNSGRLRDHPDQVRRVIRAMLQALRYLRENSEGTQQIMMSWLKLDREMATDIYQMAI
                     NNYTRNGMVEEAMLHSLVTTMLAEAGIKGIAPSQLTDFTLLQQALK"
BASE COUNT          464 a          694 c          611 g          503 t
ORIGIN      
        1 tctgtggttc agcgacctgc gcaattacac cagaattagc gactcagccg agcctgagca
       61 gattattccg ctgctgaatg aatacgccga cgcgatcctg tcggcgatca acgaatgcgg
      121 cggcgacatt ctcaagctga tgggcgacgg cacactggcg atttttaccg cggacgcccg
      181 cgccagcgct tgccacgccg cattggccgc cgccgacacc gcccgggccg cggtggcttt
      241 ggttaatcaa cggcgcagcg ccgacaattt accgatcacc gacatgtatc tcggtctgca
      301 cattggcgaa gtttactacg gcaatatcgg cagccgcgag cgcctcgatt tcaccgtcgt
      361 cggccccgcc gtcaacgaag tgagtcgcat cgccggccta tgccgctccg tcgatcaacc
      421 cctgctagtc tcatccgctt tcgcttcggc catcggcgag ccaaaaaccc ttctcgtttc
      481 ggtcggccgc tacgccctgc gcggcgtcgg caaaccccag gatctctaca cgctggatcg
      541 ccagcgcgaa aagtcagctg tcctcgacga cgacagcacg cagtaagctt catcgctttc
      601 ccgccgatcg agaagaggcc gctacactgg tggtaatcgg aacgccggtt gtccctttat
      661 gcttcggaca atggcggagg ttcccttcga cgttcaaaac cgaaatgtca ttttgtacga
      721 agcgggtcaa attcatgcgc tccgtcccaa gctattacgt cgccttcggc gctacttggt
      781 ggatggcggt ctacttcgaa cgagtggata tcctctgtca tgggatatcc ccgctatgtc
      841 aaaccttatc tacaagacta ctgcccctcg aaaccactcg ggcaaccaag tcaccggcat
      901 ctgatcctgg acgcagcgaa agcctttcac ttggttcttc cactccggca agtaacgatg
      961 cgagcagtcg catgaattcg ctggactgca caaggctctg taagtttgcc cgctaatgtc
     1021 gacgtcggtt gaagtccatt cccagatatc gccacgcttt ttgagcttgc cggcttcgca
     1081 agtcgatttc cattcatcga acgtcggcaa tcgccgtcct acgaagcggc aatagccact
     1141 cacgtcatgg aagttgacga gcacaacagg ttcggcgtct ttgccggaag gaaaacagcc
     1201 gttgacccaa tactccggcg cagcgtggcg ggtcgccagg acgaacttta gatactcgcc
     1261 gttggtgact tcgctcgggt ccaacgctgc cgcggcgaga aaatagaccg gcaagaagaa
     1321 cgcccagcaa cgaccgaaac ggtgcaagag tttcagctta ggatttggca attttatccg
     1381 tgcgatgttt gcgaatcaaa gggcgaccgc cggtcgccct tactttcctt ttgtgttctt
     1441 tgcgttcttt gcggccattc ttcttttcgg ttgcgtctac ttgagcgctt gctgcagcag
     1501 tgtgaaatcg gtgagctgcg acggcgcaat gcctttgatg ccggcttccg ccagcatggt
     1561 tgtaaccagg gaatgcaaca tggcttcttc gaccatgccg tttctcgtat agttgttgat
     1621 cgccatctga taaatatcgg tcgccatctc gcgatcaagc ttcagccaac tcatcatgat
     1681 ctgctgcgtg ccctcagaat tttcccgcag gtagcgcagc gcttggagca ttgcccggat
     1741 cacccgccgc acttgatcgg gatgatcgcg cagcctgccg ctattcacac ctagcccgcc
     1801 ggcgagcagg ccgtgatagt cggcggcatt gatcaaaagc ttgaggccca tcttcttggc
     1861 tcgaaatgct tcgccgtaat ccatcatcgc gctgtcgatg gagccggaga gcatgccgcc
     1921 gatgcgcgtg ccggtgtcga cgacgcgata aatgatgtcg cgatcgggat ccaggccccc
     1981 ttgcttgaca ataaacttgg ccgaaatctg ttgcgagccg agcagtccag gcacgcccat
     2041 cgattttccc ttgagctcgc ttaccgactg aatttcggga cgcaccacca gcccccaaaa
     2101 tggtttggtc gtgccgagaa acacgatgcg caccggcaat cctctggccg ccgatacgaa
     2161 cgtcgaaccg gctctgagcg tataatgtag gttgccgctg gccagcgcgg cgcgatccac
     2221 ctcggtgcgg gtgagcagca gcttaacgtc gaggttctgc tcgcgaaaaa ag
//
LOCUS       DASQKS010000021         4662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1691515, whole genome shotgun sequence.
ACCESSION   DASQKS010000021 DASQKS010000000
VERSION     DASQKS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691515"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VE170_00475"
     CDS             complement(<1..522)
                     /locus_tag="VE170_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYJ13932.1"
                     /translation="MFKKMVEIDASDLYLTVARPPMCRVEGKVRPIGEKNFTPDDLHA
                     LADAMMTEKQREEFARTFEMNLAQSLPGVSRFRVNILMQRGSRAMVVRRIKGDVLSLD
                     EMGLPSILKDITLAKRGLVLVVGATGSGKSTTLAAMVDYRNANEAGHIVTIEDPIEFV
                     HRHKQSIVTQREVG"
     gene            complement(568..1638)
                     /locus_tag="VE170_00480"
     CDS             complement(568..1638)
                     /locus_tag="VE170_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYJ13933.1"
                     /translation="MDITQLLAFGVEQKASDCHLSAGEPPMIRIDGDLKKLDFPALTK
                     EEVHALVYDIMSDGQRKAFEETHECDFSFALAETARFRVNVFMQRKGEAAVFRTIPTK
                     ILTLEDLGLPQIFKQLCEKEKGLILVTGPTGSGKSTTLAAMVDYLNSTYEGHILTVED
                     PIEFVHTSKKCLVNQRELGPHTHSFSNALRAALREDPDIILVGEMRDLETIQLALTAA
                     ETGHIVFGTLHTSSAPKTVDRVIDVFPPAQQAQVRSQFAESIEAVITQTLLKKKSGGR
                     TAAHEIMIGSTAVRNLIREGKIHQIPGTMQVSQKDGMQTMDMALQTLVTRGLVSREEA
                     QSKSMNPNLFGAAPAAASGGRG"
     gene            1843..2640
                     /locus_tag="VE170_00485"
     CDS             1843..2640
                     /locus_tag="VE170_00485"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ13934.1"
                     /translation="MTTESHKALLEGKVALVSGGGTGIGRAIADQLAKNGAKVAIASR
                     NRDQLKHAATELTELGLMVQSVPMNLRDQTDVERAVAQVVGQWGAIHILVNNAGISGL
                     SRMDDDDDSKWYDIVDTNLHGMYRLTKAVLRHIPDQAGGRVINISSVLGKFGVPGYTA
                     YCTTKHGMIGFSRALALEVVNRGITVNTICPGWVDTEMAAQGIAETAKSLGITPEAFK
                     AQAVAAVPIRRFLAAEEIAQLVAYVASDAARGITGQAMNICGGQTMV"
     gene            2750..4276
                     /locus_tag="VE170_00490"
     CDS             2750..4276
                     /locus_tag="VE170_00490"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HYJ13935.1"
                     /translation="MSANIQVRNPATLELIQELPVASPAEVALAITRARQAQLSWQQT
                     NFAERAKILYRLRDLLLDEGDRLADILTSETGRPRAEVYGNELFYLCNAIGAWAKHSE
                     RYLRRQKITPSFPLVKAKKVYFTYAPRGVIGIISPWNFPLTLTLGEALPALMAGNAVV
                     IKPSELTPLSARFGAELAAKAGFPKDLLQIVVGFGDTGAALIDHADMIAFTGSVETGK
                     QVMRRAADRLIPVSLELGGKDPMIVLKDADMERAAGACVWGALMNCGQTCTSIERVYV
                     EAPIYQAFVDNVATRVKAIRQGPSAEAVDIGAMTSAAQLDKVAAQVDEARAKGARIVT
                     GGRRNPTLPGYYYEPTVLVDVDHSMSIMTDETFGPVIPIMQVSSPAEAVRMANDSRYG
                     LSASIFSHDRPAAARLADQLDSGAICVNDSLVNFIIPEAPMGGRKDSGFGYRHGAAGI
                     RKFCQQKTIVVDRFGLKEEFPWYPASEKKARQVRHLLNLLCHSGWAHKLRALKGLIRS
                     "
     gene            4352..>4662
                     /locus_tag="VE170_00495"
     CDS             4352..>4662
                     /locus_tag="VE170_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13936.1"
                     /translation="MAKDLPSFIAKEGATREGAVIREKNHIDANNYETVAYLKHLDMR
                     NEPKMVLFENLPALNGEKSAFPMFYNPWVSRQYIADSLDMGDIKSPMEVSLEVARLEQ
                     Q"
BASE COUNT         1046 a         1339 c         1280 g          997 t
ORIGIN      
        1 gccgacctcg cgttgggtga cgatcgattg tttatgccga tggacgaact cgatggggtc
       61 ttcgatggta acgatgtgtc cggcttcgtt ggcgttacgg taatcgacca tcgccgccag
      121 agtggtcgac ttaccggacc cggtcgcgcc taccaccaat accagaccac gtttcgccag
      181 ggtgatgtct ttgaggatcg acggcagacc catctcatcg agcgacagca catcgccctt
      241 aatacgccgc acgaccatcg cccgggagcc tctttgcatg agtatattga cacgaaagcg
      301 cgacacaccc ggcaacgatt gggcgagatt catttcaaac gttcgggcaa actcctcacg
      361 ctgcttttcc gtcatcatgg catcggccag agcgtgcagg tcgtccgggg taaagttttt
      421 ctcaccgatc gggcgcacct tgccttcaac gcgacacatc ggcggccgcg ccaccgtcag
      481 atagagatcg gaagcgtcga tctcgaccat ttttttgaac atttctttga agtccaagaa
      541 tccccccaag ctaatccggc cggccgatta gccccggcca cccgacgccg ccgccggcgc
      601 ggcgccgaat agattcggat tcatactttt cgattgtgcc tcttcgcggc tcaccaggcc
      661 ccgggtaacc agtgtctgta gcgccatgtc catggtctgc atgccatctt tttggctcac
      721 ctgcatggtg ccggggatct gatggatctt gccttcgcga atcaggttgc gcaccgcggt
      781 gctcccgatc atgatttcat gagcggcggt gcgcccgccg cttttcttct tgagcagggt
      841 ttgcgtgatc acggcttcga tggactcggc gaactgggag cgaacctggg cttgttgggc
      901 cggcggaaaa acgtcgataa cacgatccac cgtcttcggc gcgctcgaag tatgcaacgt
      961 gccaaagacg atgtgaccgg tctccgcggc cgtgagggcg agctgaatgg tttcaagatc
     1021 gcgcatttcg ccaaccagga taatatccgg gtcttcacgc agtgccgcgc gcagagcgtt
     1081 ggaaaacgaa tgagtatgcg ggcctaactc gcgttggtta accaagcact tcttcgatgt
     1141 gtggacgaac tcgatgggat cttcgacggt gagaatatgc ccttcataag ttgaattgag
     1201 ataatcgacc atcgccgcca gcgtcgtcga cttacccgac cctgtgggcc cggtgacgag
     1261 aatcagcccc ttttcttttt cacaaagttg cttgaagatt tgcggcaatc ccaaatcctc
     1321 cagggttaaa atctttgtcg gaatcgtccg aaagaccgcg gcttcacctt tgcgttgcat
     1381 gaaaacgttg acgcggaaac gagcggtctc cgcgagggcg aaagaaaaat cgcactcatg
     1441 ggtttcttca aaggctttgc gctgaccgtc gctcatgatg tcataaacca gcgcatgcac
     1501 ttcttccttg gtcaacgccg ggaagtcgag cttcttcagg tcgccgtcga tgcggatcat
     1561 cggcggctcg ccggcgctca aatgacaatc cgatgccttc tgttcgacac caaaggccaa
     1621 caattgggta atatccatga atcacacctc taaatcggct aaagcttgag agagattttg
     1681 ctatgtcggt gaaagttata aaaaaacttg gtcgtacttg tctggttttg atcagcaagt
     1741 tcaataccat taggtggttt gagataatcg ggccttttac cggcacggga tagatattag
     1801 cgaggcattg aaattcgctg taacaccagc tacttaagtt aaatgactac ggaatcgcac
     1861 aaagccctgc tcgaaggtaa agttgccctt gtcagcggcg gcggtacggg aattggtcgg
     1921 gcgatcgccg accaattggc caaaaatggc gccaaggtcg ccatcgcctc gcgtaatcgg
     1981 gatcagctca agcacgccgc gacggagcta accgagctcg gcttgatggt tcagtccgtg
     2041 cctatgaatc ttcgggacca aaccgatgtt gaacgcgccg tcgctcaagt tgtcggccaa
     2101 tggggcgcga tccatatctt agtgaacaat gccggcatct cggggctgag tcgcatggac
     2161 gatgacgacg actccaagtg gtacgacatc gtcgatacca atttgcatgg catgtatcgg
     2221 ctcaccaaag cggtgctgcg ccacattccg gaccaagccg gcggccgcgt gatcaatatc
     2281 tcatcggtgt tgggaaaatt cggcgtccca ggttacaccg cctattgcac gaccaaacat
     2341 ggcatgatcg gcttcagccg cgcgttggct ctcgaagtcg tgaaccgcgg catcacagtc
     2401 aacaccattt gtcctggctg ggtcgacacc gaaatggccg ctcagggaat cgcggaaacg
     2461 gcgaagtctt tgggcattac gccggaagcg ttcaaagcac aggcggttgc cgcggtgccg
     2521 atccgaaggt tcttggcagc cgaggagatc gcccaactgg tcgcctatgt cgcctcggat
     2581 gccgctcgcg gcattaccgg acaggcgatg aatatatgtg gcggtcagac catggtgtag
     2641 atttgccgtt gctcgcggta gccgcagacg ccgtgcgaac gtagcaaaca acgcagaagt
     2701 ctcgcgacaa ctaaaggctt tttccagccc tgaacttttg gctaatcaaa tgagcgcaaa
     2761 cattcaagtt cgcaatcccg ctaccttgga gttgatccaa gaactaccgg tggcatcgcc
     2821 agccgaagtc gccctggcaa tcacccgcgc ccggcaggca cagttgagtt ggcaacaaac
     2881 aaattttgcc gagcgggcaa aaattctcta ccgactgcgt gacttactgc tcgatgaagg
     2941 cgatagactc gccgacattt tgaccagcga aaccggccgg ccgcgcgcgg aggtctatgg
     3001 taacgagcta ttctacttgt gtaatgccat tggcgcctgg gccaagcact ctgagcgcta
     3061 ccttcggcga caaaaaataa cgccgagctt tcctttggtg aaggccaaga aagtttactt
     3121 cacctacgcg ccgcgcggag taattggcat catcagccca tggaactttc ccctgactct
     3181 gaccttgggc gaggccctgc cggcgctgat ggccggcaat gccgtggtga tcaaaccttc
     3241 ggagctgact cctttatccg cgcgctttgg cgccgaattg gccgccaagg ccggctttcc
     3301 caaagatctg ctgcaaatcg tcgtcggctt cggcgacact ggcgccgccc tcatcgatca
     3361 tgccgatatg atcgccttta ccggcagcgt cgaaaccggc aaacaggtca tgcgccgcgc
     3421 cgccgaccgg ctgattcccg tctcattgga gttgggtggc aaagatccca tgatcgtcct
     3481 aaaggacgca gacatggaac gagcggcggg cgcctgcgtc tggggtgcat tgatgaactg
     3541 tggccaaacc tgcacctcga ttgagcgcgt ctatgtcgaa gcgccaattt atcaagcgtt
     3601 cgtcgacaac gtcgccaccc gggtcaaagc gatccgtcag ggacccagcg ccgaggccgt
     3661 cgatatcggc gccatgacat cggcggcgca gcttgataaa gtggccgcac aagtcgacga
     3721 agcgcgcgcc aaaggcgcca ggatcgtcac cggcggtcgg cgcaatccca ctttgccggg
     3781 ctattactac gagccgacgg tactcgtcga tgtcgatcat tccatgagca ttatgaccga
     3841 tgaaaccttc ggcccggtaa ttccgatcat gcaggttagt agtccggcag aagccgttcg
     3901 aatggccaac gattcgcgtt acggcctgag cgccagcatc ttttcgcacg accggccggc
     3961 ggcggcaagg cttgccgatc aacttgacag cggcgcgatt tgcgttaacg acagtctggt
     4021 caactttatc atccccgaag cgcccatggg cggacgcaaa gacagcggct tcggctatcg
     4081 ccacggcgcc gccggaattc gcaaattctg ccagcaaaaa accatcgtcg tcgaccgttt
     4141 cggccttaag gaagaattcc cttggtatcc cgcgagcgag aaaaaagccc gccaagtgcg
     4201 ccatctgctc aacctacttt gccactcggg ttgggcgcac aaactgcgcg cgctcaaggg
     4261 gcttattcgg agctagctcg cttgcactca cgcggcgatt catctagtac aattgatatt
     4321 cccaggtaaa taaattcaca ggagaatttt catggccaaa gatctgccga gttttatcgc
     4381 caaagaaggc gccacccgcg aaggcgcggt gattcgcgaa aaaaatcata tcgacgcaaa
     4441 taattatgaa accgtcgcat acttgaaaca cctggacatg cgcaacgaac cgaaaatggt
     4501 gttgttcgag aatctgccgg cgctgaatgg cgagaagtcc gccttcccga tgttctataa
     4561 tccctgggta tcacgccagt acatcgccga ctccctcgac atgggcgata tcaaatcacc
     4621 catggaagtc agtctcgagg tcgccagatt ggaacagcag ca
//
LOCUS       DASQKS010000022         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_432429, whole genome shotgun sequence.
ACCESSION   DASQKS010000022 DASQKS010000000
VERSION     DASQKS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432429"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VE170_00500"
     CDS             <1..412
                     /locus_tag="VE170_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HYJ13937.1"
                     /translation="QRLYFYKRLASLKNAVELDELKAEIEDRFGPYSEVVDNLFLVMN
                     LRRVLKEFLVQQISFSDGRIFLLFHRDSPVKVDKLLELIHNQKHRFRLAPDGRLSFTP
                     KRSDWQPLAGEVIELLHGLREIPAPKKAQWIPAG"
     gene            592..1896
                     /locus_tag="VE170_00505"
     CDS             592..1896
                     /locus_tag="VE170_00505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ13938.1"
                     /translation="MLIRAKWVGLWLVALVVMPQGLSAAVVEQLIAVIDGEPYTLTNL
                     TTFAKSRMATTFPTGSLQEINAADREVLEQFITDKLMEAEVREAGIRIMDDDVNRYIE
                     QVKKSNRLSDEDFKASLAREGLSLASYKISVKTEMEKAEIVDRQVKRKVNITDEDVER
                     YYKLNSKNYRANERARIRHILLSLPEKAPADQVTAVSAKAKELHKRIIGGEDFAAVAR
                     EYSEGAGRADGGDIGWVNKGTLIAGLEEVAFEKLSPGKISEPFRTSMGMHIVKLEARE
                     AGSVLPLSAVAAKIKQELLAKAMEERFAKWLKSDLRRKHRVDVKLAGVTFKPEESKEE
                     TVNALMARSNRPARRESRSALSYLNPLSYIVNETPFEDEDPKSPLSNKSVVSIFGMPL
                     FVTENADDTPDIFAPPAPEPEKSKGIFSSIVDSLNPFSSKKP"
     gene            1963..2985
                     /gene="pdxA"
                     /locus_tag="VE170_00510"
     CDS             1963..2985
                     /gene="pdxA"
                     /locus_tag="VE170_00510"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYJ13939.1"
                     /translation="MPYKPIIAVTMGDPSGIGPEIILKALADPIIKRVARPLILGDWG
                     VLQRTHVRRKRPKLLCWQPGQRLLPMLDDPKAFTVCSLSALSARDSRPGVAAKAAGHG
                     TFSYIRVAAKLALSKVADAIATAPLSKNILIDAGYDYPGHTELFAELGGTPECRMMLI
                     GAKLRVVPVTGHIPFAKVARSLNGENLQTTFALTHASLKKSFGIARPRIAVAALNPHG
                     GEKGNFGAEEIDIIAPAVKAARKHGIPVQGPFPADSLFHHAARGDYDAVVCMYHDQGL
                     IPLKLHHFYGGVALTLGPPFIRTSVDHGTAYDIAGKDQADETSMKEAILLAVRLARQN
                     HRPKKT"
     gene            2982..4337
                     /locus_tag="VE170_00515"
     CDS             2982..4337
                     /locus_tag="VE170_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYJ13940.1"
                     /translation="MNPAVFREYDIRGLAEIDFDKDFALLLGKVHGTLIAETGGTRAT
                     VGRDCRATSDAYAEAVIAGLVSAGLQVYDLGVCPSPLLYFSLFYLDVDGGIQITASHN
                     PSEYNGFKICLGKDSQYGEQIQELRQKMERGEFRQRPGGQVASYDILPAYQQRLLTDV
                     PKLARPLKIVVDAGNGVGGPVAPPIFRHLGCQVWEIGCVPDGNFPLHHPDPTVPENLA
                     MLIAKVRQEKADLGVAYDGDADRIGAVDEQGNIFWGDELLILFARDMLKRNPGAVVIS
                     EVKCSQRLFDDIAKHGGKPIMWKAGHSLLKAKMKETGALLAGEMTGHMFFKERYYGYD
                     DAIYASLRLLEILADAGKPLSTLLADLPNSVSTPELRVECPDDKKFIIADRATAYFRR
                     HYDVIDIDGVRVQFAEGWGLIRASNTQPALVLRFEARSAAKLKEYRTLIEGKLREFEL
                     Q"
     gene            4344..>4663
                     /locus_tag="VE170_00520"
     CDS             4344..>4663
                     /locus_tag="VE170_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ13941.1"
                     /translation="MAGQIVIQGARVHNLKNIDLEIPRDQLVVITGVSGSGKSSLAFD
                     TLYAEGQRRYLESLGADTRQRLKQLAKPDVDRIDGLSPAIAIQQKAAAHNPRSTVGTL
                     SDIY"
BASE COUNT         1049 a         1305 c         1336 g          973 t
ORIGIN      
        1 ccagcggctg tatttttaca agcgcttggc gagcctgaag aacgccgtcg agctcgacga
       61 gttgaaagca gagatcgaag accgctttgg tccctacagc gaagttgtcg acaatttgtt
      121 tctggtgatg aatctacgcc gagtgctcaa agagtttctc gttcagcaga tcagcttctc
      181 cgacggcagg atattcttgc tcttccatcg tgattcgccg gtcaaagtcg acaagctgct
      241 cgaactcatc cataatcaaa aacatcgttt tcgcctggcg cccgacggcc gcttatcgtt
      301 cacgccaaag cgatccgact ggcagccgtt ggccggtgaa gtgatcgagt tgctccacgg
      361 gctccgtgaa atcccggctc ccaagaaagc ccagtggata ccggcggggt gaaaaaaaga
      421 gcgtgcggtg ccggctccgc agcgtttagc ggcgcaactt cggcaaatat taataaggat
      481 tgaccgcggc ggggtctaag tgctgtaaag tatcgacact cctggtgttt gccggtttgg
      541 aattcaaaat cgtctatgtt acctgttagg tagtttggct gggggtttgc gatgttgata
      601 cgagcgaaat gggtcgggtt atggctggtg gcgctagtcg ttatgccgca aggcctatca
      661 gcggccgtcg tcgagcagtt aatcgctgtg atcgacggcg aaccctacac ccttaccaat
      721 ctcactacct ttgccaagag ccgcatggcg acaacgttcc cgacagggag tttgcaggag
      781 atcaatgccg ccgaccgcga agtcttggag cagttcatca ctgacaagct catggaagcg
      841 gaagtgcgcg aagccggaat tcgtatcatg gacgatgatg tcaatcgcta tatcgaacag
      901 gtcaagaaaa gcaatcgact ttccgatgag gatttcaagg cgagtcttgc ccgcgaaggg
      961 ctgagtctgg cgagctacaa aatctcggtt aagaccgaga tggaaaaagc cgaaatcgtc
     1021 gaccggcaag tgaagcgcaa ggtcaatatc accgacgaag acgtcgaacg ctactacaag
     1081 ctcaactcga agaactaccg ggccaatgaa cgggcgcgga ttcggcatat cttgttgtcc
     1141 ctgccggaaa aagctcccgc cgaccaagtc accgccgtgt ctgccaaggc taaggagcta
     1201 cataaaagaa ttatcggagg cgaggacttc gccgccgtgg cgcgggaata ttccgagggc
     1261 gccggtcgcg ccgatggcgg cgacatcggc tgggtgaaca agggtacatt gattgccggg
     1321 ctcgaagaag tcgcctttga aaaactcagt cccggtaaaa tcagcgagcc gttccgcacc
     1381 agcatgggaa tgcatatcgt gaaactggaa gcgcgcgaag ccggtagcgt cttgccgctc
     1441 agtgctgtag ccgcgaaaat caaacaagag ctgctcgcca aagccatgga agagcgcttc
     1501 gcgaagtggc tcaaatcgga tctgcgccgc aaacaccgcg tcgacgtcaa gctcgccgga
     1561 gtgacgttca aaccggaaga atcgaaggaa gaaaccgtca acgcattgat ggcgcgttca
     1621 aaccggccgg cgcgccgtga atcgcgctcg gctttgagct atctgaatcc gctttcctac
     1681 atcgtcaacg agacgccatt tgaggacgaa gatcccaaga gtccgttgtc gaacaaaagc
     1741 gtcgttagta tctttggtat gccgctattc gtcactgaaa acgccgatga tacgccggat
     1801 atcttcgcgc cgccggcgcc ggaaccggag aagtccaagg ggatattctc gtcgatcgtc
     1861 gattcgttga atccattctc gtcaaaaaag ccttaatctt tgcctcatcg ttcgtaagtg
     1921 gagatactcg attaactatc tcgcccgacg ttgcgccacc gcatgcccta taaacccatt
     1981 atcgccgtta ccatgggcga tccgtcggga atcggccccg aaatcattct caaggcgctc
     2041 gccgacccta ttatcaagag agttgcccgg ccgctgattt tgggcgactg gggcgtattg
     2101 caacgaaccc atgtgcgccg caaacggccg aaactgctct gctggcagcc tggccagcgg
     2161 ctgttgccga tgcttgacga tcccaaagcg ttcacggttt gttcgctgtc ggcgctgagc
     2221 gcaagagact cccgacccgg tgtcgccgcc aaagccgccg gccatggcac cttcagctat
     2281 atccgggtgg cagccaagct ggcgctttca aaagtcgccg atgccatcgc cacggcgccg
     2341 ctgagcaaga atatcctgat cgatgccggt tacgattatc ccggccacac cgaattgttt
     2401 gccgaactcg gcggcacgcc cgaatgccgt atgatgctga tcggcgctaa gctgcgcgta
     2461 gtgccggtga ccggccatat tccattcgct aaagtagcgc ggagtttgaa cggggaaaat
     2521 ttgcaaacga cttttgcatt gacccatgca agcttaaaaa aatccttcgg catcgcccgg
     2581 ccgcgaatcg cggtggcggc cctcaatcct cacggcggtg aaaaaggcaa cttcggcgcc
     2641 gaggagatcg acatcatcgc gccggcggtc aaggcggcgc gcaagcatgg gattccagtt
     2701 cagggaccgt ttcccgcgga tagtttgttt catcatgccg cgcgcggcga ttacgacgcg
     2761 gtggtgtgca tgtatcacga tcaaggttta atcccgctca aattgcacca tttctacggc
     2821 ggtgtggcac tgacgctggg accgccattt atccgcacct cggtggacca tggcaccgcc
     2881 tacgatatcg ccggcaagga ccaagccgat gaaaccagca tgaaggaagc gattctcttg
     2941 gcggtgcggc tcgcgcggca aaatcatcgg ccaaaaaaaa catgaaccca gcggtgtttc
     3001 gcgaatacga cattcgcggt ctagcggaaa tcgatttcga caaagatttc gcgctgctgc
     3061 tcggcaaggt gcatggaacc ttgatcgcgg aaacgggcgg cacacgcgcc actgtggggc
     3121 gagactgccg ggcgacttcc gatgcttatg ccgaagctgt gatcgcaggc ctggtgtcgg
     3181 ctggcctgca agtctacgat ctcggcgtgt gtccgtcgcc actattgtat ttctctctgt
     3241 tttacttgga cgtcgatggc ggtattcaaa tcacggccag ccacaatccg tctgagtaca
     3301 acggctttaa aatttgcctg ggcaaagaca gccagtatgg cgagcagatt caagagctgc
     3361 gccagaagat ggagcgcggc gaatttcggc agcggccggg cggtcaagtg gcgagttatg
     3421 acatcctgcc ggcctatcag cagcgtcttt taaccgacgt gccgaagctg gcacgcccgt
     3481 taaaaattgt cgtcgatgcc ggtaatggcg tcggcggtcc ggtggcgccg ccgatttttc
     3541 gccacttggg ttgccaggtg tgggagatcg gttgcgtgcc ggatggtaat tttccgttgc
     3601 accatcctga tccgacggtg ccggaaaatc tggccatgtt gattgccaaa gtcagacaag
     3661 agaaagccga tctcggcgtc gcctacgatg gcgacgccga ccgtatcggc gccgtcgatg
     3721 agcagggcaa tattttctgg ggcgacgagc tactgatcct gtttgcccgg gatatgctca
     3781 agcgcaatcc cggcgcggtg gtgatctcgg aggtgaaatg ttcgcagcgg ctgtttgacg
     3841 acattgccaa gcatggcggc aagccgatta tgtggaaggc cggccattcc ttgctcaagg
     3901 cgaagatgaa agagaccggc gcgctgttgg cgggcgagat gaccggccat atgtttttca
     3961 aagaacgcta ctatggctat gacgacgcca tctatgcctc gctccgcctg ttggaaatac
     4021 tcgccgatgc cggcaagccc ctgtcgacac tgctcgccga tctaccgaat agcgtgtcga
     4081 cgccggaatt acgcgtcgag tgccccgatg acaaaaagtt tatcatcgcc gacagggcca
     4141 cagcatattt tcgccggcat tatgatgtca tcgatatcga cggcgtgcgg gtgcaattcg
     4201 cggaaggctg gggtctgatc cgcgcttcca atactcaacc ggccctggtg cttcgtttcg
     4261 aagcgcgttc ggccgccaag ctcaaggaat atcgcacgct gatcgaaggc aaactccgag
     4321 aattcgagtt gcagtagttt cagatggcgg gccaaatcgt catccagggc gcgcgtgtcc
     4381 acaatctcaa gaatatcgat ctggagattc cgcgcgacca gttggtcgtc ataaccggtg
     4441 tttccggctc cggtaagtcc tcgctcgcct tcgataccct ttatgccgaa ggacaacggc
     4501 gctatctcga atctctcggc gccgacaccc gccaacgcct caagcaactg gccaagcccg
     4561 atgtcgatcg catcgatggg ctgtcgccgg ccatcgcgat acaacaaaaa gccgccgcgc
     4621 acaatccccg ctccaccgtc ggcacgctca gcgatattta tga
//
LOCUS       DASQKS010000023         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2532404, whole genome shotgun sequence.
ACCESSION   DASQKS010000023 DASQKS010000000
VERSION     DASQKS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532404"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VE170_00525"
     CDS             <1..933
                     /locus_tag="VE170_00525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ13942.1"
                     /translation="GSYPQLLVARVAIDAGSSPQHPLGASILSRYYPKARGWALTFHH
                     SAGSFGSFIGPGLASIALLYMDWRTAFVVFGVASLVMGLTLFLVSEHSAAADGVAGGK
                     DRASNFKANLAAYLICLKDRNIMLTSLVLMVGAAGRGTGINLTYLVPFFMERFGVSAP
                     MGGLFLTVLQGAGLVGPLAIAWVSDRVGRRALITQVTLFLSAIMTVCLAYQTSLGALF
                     YLNLILYGSVVEARSSLTQTMISEYAKDGLTDAAFSIYYFVGCISGPIWTLIVGYIMQ
                     QHGFTTAFLVAASTYLAGMLLLTFVKEEPKTAKA"
     gene            1200..2066
                     /locus_tag="VE170_00530"
     CDS             1200..2066
                     /locus_tag="VE170_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ13943.1"
                     /translation="MTVQIIALSAAITYALSFILSKRGFKNSTPITITFVSLLIQSVT
                     LLTIALTVTGIPKVTPFVFLLFAIAGVLQAVVRMLTYIGIEKIGAARSGPIRASVPIW
                     SAAIAIFFLGETVNAAIGLGTILVVGGILLISWRADEHVKDFRPWFIIAPLLAAILGG
                     VVYPIRRYALRFSDEPIFFGAVVGSVGLFCTAAYLALPTTKEKLVWNRASIGYFIAGG
                     AFESLGLLLVLYALSFGPVVMVTPLTATLPLWVVLLSKMFLGDVEKITSRIVVGAGLV
                     VAGTIAISLAKT"
     gene            2439..>2798
                     /locus_tag="VE170_00535"
     CDS             2439..>2798
                     /locus_tag="VE170_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13944.1"
                     /translation="MRRIILTVFIALLAITAAMDTFAAAPRPLRIAYLFTSGTMASMW
                     MAKESGGFAKEGLDVEMISMSSVLALPALIANEVDVIQVSAVPVITASLRGFDVVFIS
                     GMLNTMVWDLYARPEIKT"
BASE COUNT          507 a          846 c          752 g          693 t
ORIGIN      
        1 ggcagttatc cgcagctttt ggtcgcccgg gttgccatcg atgccggatc gagcccgcag
       61 catcccctcg gcgcgagcat tttatcgcgc tactatccca aggcgcgcgg ttgggcattg
      121 acgtttcatc attcggctgg aagtttcggt tctttcatcg gcccgggcct cgcgtcgatc
      181 gccttgcttt acatggattg gcgaaccgcg ttcgtcgttt tcggcgtcgc cagcctggtc
      241 atgggactta cgctcttttt ggtcagtgag cattcggcgg cggccgacgg tgtcgccggc
      301 ggcaaagacc gcgcgagcaa cttcaaagca aacctcgcag cctacctgat ctgcctcaaa
      361 gaccgtaaca tcatgctcac ctctctggtt ttaatggtag gtgcggcggg ccggggcacc
      421 ggcatcaacc tgacttatct cgtgccattt ttcatggaac gcttcggcgt ttcggcgcca
      481 atgggcggat tattcctgac cgtactgcaa ggcgctgggc tggtgggccc cctggccatc
      541 gcctgggttt ccgaccgggt gggacggcgc gctttgatta cccaagtaac gttgttcctg
      601 tcagcgatta tgaccgtttg tctcgcatat cagacttctc tcggtgcgct cttctattta
      661 aacctgatac tctacggtag cgtcgtcgag gcgcgcagct cactcactca aaccatgatc
      721 agcgaatacg ccaaagacgg cctcaccgac gcggcttttt cgatctatta tttcgtcggc
      781 tgtatctcgg gtccgatctg gaccctgatc gtcggctaca tcatgcaaca gcatggcttc
      841 accacggcgt tcttggtcgc agcatcgact tacttggccg gcatgttgtt gttaacgttc
      901 gtcaaggaag aacccaagac cgccaaagcc tgatggaagt ttggaatttc cccgcagggg
      961 cgctgcgcgc gtagagacca ttgattggct ttcagattcg gaggtccctc gcgcaaagac
     1021 gtaagacgct acgtgacggg ccgcagcctg tcgtacccaa cgaacgtgag cgatcgagaa
     1081 ttctcgctcc ggttgaaatg acatctccca attgcggctt agcgtcgttt cggaagatct
     1141 tctgattttg tgtcctttgc gttctttgcg gccaatcctc ccatttcgaa ccactcttca
     1201 tgaccgtcca gatcatcgct ttgtcggcag ccatcaccta cgccttgagc tttattctct
     1261 ccaagcgcgg gtttaagaat tccacgccga tcaccattac gtttgtctcc ttgttgattc
     1321 agagcgtcac gttattgacc atcgcgctca ccgtcaccgg cattcccaag gtaactccgt
     1381 ttgtgttcct gcttttcgcc atcgccggcg tcttgcaagc ggtggtgcgc atgctgacct
     1441 acatcggcat cgaaaaaatc ggcgccgccc gcagtgggcc gatccgcgcc tccgtgccga
     1501 tctggagcgc ggcgattgca attttcttcc tcggcgagac cgtcaacgcc gccatcggtc
     1561 tcggcactat tctcgttgtc ggcggaatct tactgatctc ctggcgcgcc gacgagcatg
     1621 tcaaagactt ccgtccgtgg ttcatcatcg caccactgct ggcggcgatc ctcggcggcg
     1681 tggtctaccc gatccgccgc tatgcgctgc ggttcagcga cgagccgatt tttttcggcg
     1741 ccgtggtcgg cagcgtcggc ttattctgca cggccgcgta cctcgccctg cctacgacca
     1801 aggagaaact cgtctggaac cgtgcatcga tcggctactt catcgccggc ggcgccttcg
     1861 aatccttagg gcttctgctc gtgctctacg ccctcagctt cggccccgtc gtcatggtca
     1921 cgccgctcac cgcgaccttg cccctatggg tcgtcctgct cagcaagatg tttctcggcg
     1981 acgtcgagaa aatcaccagc cgcatcgtcg tcggcgccgg gttagtagtc gcgggaacga
     2041 ttgccatctc acttgcgaag acctaacaac tgcgacaact tccttctggc agcttcggca
     2101 acctaccttt acctggcaac cggcaccgta tacggagcgc gcggagcgat tcgaaccgtc
     2161 aaggctttaa ctcttgtcct ggccgtcgcc gctatcttat tgggccatcg atttcgtatt
     2221 atttctgctg actttgtata cgacctagca atgggagtga aattagcggc tccgggtcag
     2281 cgcgctcgag ctgagacgtg aaagagttga cctatcgagg caagctggca gatattacga
     2341 ctatcaagat ttcagcgcga gctagagcag taacctgtcg tgcccggatt gtttgctttg
     2401 gccgattggt taccttgaca cggaacggga ggcacaattt gcgacgtata atcttaacgg
     2461 tattcatagc ccttctcgcc attacggcag cgatggacac gttcgccgcc gccccgcgcc
     2521 cgctacgaat cgcctatcta ttcacttcgg gcacgatggc gtcgatgtgg atggccaagg
     2581 aaagcggcgg cttcgctaaa gaaggccttg acgttgagat gatttccatg tcctcggttc
     2641 tcgccctccc cgccttgatc gccaacgagg tcgacgtcat tcaagtatcg gcagtgccgg
     2701 tgatcaccgc ttcgctgcgc ggcttcgatg tggtgtttat ttccggcatg ctcaacacca
     2761 tggtctggga tttatacgca aggccggaaa tcaaaacg
//
LOCUS       DASQKS010000024         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_853161, whole genome shotgun sequence.
ACCESSION   DASQKS010000024 DASQKS010000000
VERSION     DASQKS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853161"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VE170_00540"
     CDS             <1..894
                     /locus_tag="VE170_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ13945.1"
                     /translation="VVRGLRTLATLAPIADEVIVYPNRPREPDEHDYAIAFAIPMNTK
                     GLKILCRDLYAEHADPERQPLTTRFDEVDAALIFDDVVVPWERMFVYKDPQLLSGIMY
                     IHTWGQYSTMLRLVTKLEAFLGVAQILTRYAKRNSSPPSQILLASLMQDIEILRSCIQ
                     VGESKGYRAGGGTWAPLLSSAYRVHSIEASDRAERTMEGLLTSTLMLSGGASDLNSAD
                     LGPFVERYFRGGAPDTKEHLRLMAVAADMVMTPFGKRSQLYERLQSGEVDRMRQRLYG
                     SYQDPAPAERMMQFVKAMDKD"
     gene            924..1394
                     /locus_tag="VE170_00545"
     CDS             924..1394
                     /locus_tag="VE170_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HYJ13946.1"
                     /translation="MNKTKTKKALRRIVVRRSPIHGRGVFAVRPIPKGTRIIEYKGKL
                     ITDKEADRRFGRLHENSSHTMLFSVDDGLVIDATRGGNSARWINHSCAPNCEIEEENH
                     RVFIEARRDIRPGEELAYDYNLQIGEKHTKKAKREHGCLCGARRCRGTMLGEDE"
     gene            1682..2656
                     /locus_tag="VE170_00550"
     CDS             1682..2656
                     /locus_tag="VE170_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HYJ13947.1"
                     /translation="MKITVIDDYQDAFRQLPCFAQLAGHDVEIFNDTLKDPARLAERL
                     RDAECVVLTQQRTWFRRPLLEALPNLRLISQTGRITAHIDLKACTEGGVMVCAGGRGR
                     PHSTAELTWALILAALRHVPQEVYGLRQGRWQSTLGVDIFGKTLGVYALGGIGSIVAT
                     IGQAFGARVLCWGRGASLERARLAGYEVAASRAEFFAESDILTLHLPMVRETRGIVTR
                     ADLARMKPTALLVNTSRAKLIEDGALVDGLKARRPGFAAVDVYEDEPIFDADHPLLKL
                     DNAICTPHLGFVTAETYEIHYGLAVQQILAYLAGEPIHVANPEVLPRR"
BASE COUNT          541 a          760 c          823 g          538 t
ORIGIN      
        1 gttgtgcgcg gcttgcgcac gctggcgacc ttggcgccga tcgccgacga ggtcatagtt
       61 tatccgaacc ggccgcggga accggacgag catgattacg ccatcgcctt cgcgatcccg
      121 atgaacacca agggattaaa aattctctgc cgcgatcttt acgccgaaca tgccgatccc
      181 gagcgccagc cgctgaccac gcgcttcgac gaagtcgatg cggcgctgat cttcgacgac
      241 gtcgtcgtgc cctgggagcg catgttcgtc tacaaagatc cgcagctgct ttccggcatc
      301 atgtacattc acacctgggg gcagtactcg acgatgttgc gtttggtgac caagttggag
      361 gcttttcttg gcgtggcgca gatcctgaca cggtatgcca agcgcaacag cagcccgccg
      421 tcgcaaatct tgctggcgag tctaatgcag gacatcgaaa tcctgcgctc gtgcattcaa
      481 gtgggcgagt ccaaaggcta ccgcgccggc ggcggcacct gggcgccgct gttgagctcg
      541 gcgtaccggg ttcacagcat cgaagcgtcg gatcgcgccg agcggaccat ggaaggtttg
      601 ctgaccagca cgctgatgtt gagcggcggc gcgtcggact tgaacagcgc cgacctcggg
      661 ccgttcgtcg agcgctactt tcgcggcggc gcgcccgata ctaaagagca tttgcgtctg
      721 atggcggtgg cggcggacat ggtaatgacg ccgttcggca aacgcagcca gctctacgag
      781 cgtttgcaga gcggcgaagt cgaccgcatg cggcagcggc tttatggctc gtatcaggac
      841 ccggcgccgg ccgagcggat gatgcaattc gtcaaagcaa tggataagga ttaagcgcaa
      901 ggcacacgca aaattcggga cctatgaaca agactaagac taagaaggct ctccggcgca
      961 tcgtcgtgcg ccgctcaccg attcacggcc gcggcgtgtt cgccgtgcgg ccgattccca
     1021 agggcacgcg gattatcgag tacaaaggca agttgatcac cgacaaggaa gccgaccggc
     1081 gctttggccg gctgcatgag aattcctccc acaccatgct gttctcggtt gacgatgggt
     1141 tggtgatcga cgccacccgc ggcggcaatt cggcgcgctg gatcaatcat tcttgcgcgc
     1201 ccaactgcga gatcgaggaa gaaaatcatc gcgtgtttat cgaagctcga cgcgatattc
     1261 gccccggcga agaactcgcc tatgattaca atctacagat cggcgaaaag cacaccaaga
     1321 aggccaagcg tgaacacggc tgcctgtgcg gcgcgcggcg ctgccgcggc acgatgttgg
     1381 gcgaggacga gtgatcgaaa agttttggcc acaaaagagc acaaaggtca caaaggtcac
     1441 aaaaaatcgg attcggacga atggccgcaa agaacgcaat tggcactaag ttaattccag
     1501 aagccatccg aatgtagggg cgtatggcac acgccccggg gcggaggagt gttggtcgcg
     1561 gtcttgaatg tgcgtaattc ggaattcatt tgcgttaatc gtatcgtaat tatgacgttt
     1621 ggtatttcct ccgtgccctc agcgtctctg cggtgaaaat cagtcaacca attccttgcc
     1681 aatgaagatc accgttatcg acgattacca ggatgcgttc cggcagctgc cctgctttgc
     1741 ccagcttgcc gggcatgatg tcgaaatttt taacgacacg ctcaaggatc cggcgcggct
     1801 agccgaacgg ctgcgcgatg ccgaatgcgt tgtcttgaca cagcagcgca cctggttccg
     1861 ccgtcctttg cttgaggcct tgcccaactt gcgactgatc agccagacgg ggcggataac
     1921 cgcgcatatc gatctcaaag cgtgcaccga aggtggcgtc atggtgtgcg ccggcggcag
     1981 aggtcggccg cattccaccg cggagctgac ctgggcactg atactcgcgg cgctgcgcca
     2041 tgttcctcaa gaagtctacg gactacgcca gggacgctgg cagtcgacct tgggtgtcga
     2101 tatcttcggc aagacgttgg gcgtgtacgc tctcggcggt attggctcca tcgttgcgac
     2161 tatcggccag gctttcggcg cgcgggtgct ttgctgggga cgcggcgcgt cgttggagcg
     2221 ggcgcggctc gccggttatg aagttgccgc gagccgcgca gagtttttcg cggagtcgga
     2281 tattttgacc ttgcacttgc cgatggtgcg cgagactcgc ggcatcgtca cgcgggcgga
     2341 tttggcgcgt atgaagccga cggcgctgct ggttaatacc agccgggcga aattgatcga
     2401 agacggtgct ttggtcgacg gcttaaaagc gcggcggccg ggattcgccg ccgtcgatgt
     2461 ctacgaagat gaaccgatct tcgatgccga ccatccgttg ctcaaactcg acaacgcgat
     2521 ctgcacgccg catctcggtt tcgtcaccgc cgaaacctac gagattcact atggcttggc
     2581 cgtgcagcag atcttggcgt atctcgccgg cgagccgatt catgtggcta atccggaagt
     2641 gttgccgcgc cggtgattca ta
//
LOCUS       DASQKS010000025         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5473869, whole genome shotgun sequence.
ACCESSION   DASQKS010000025 DASQKS010000000
VERSION     DASQKS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5473869"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..913
                     /locus_tag="VE170_00555"
     CDS             107..913
                     /locus_tag="VE170_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ13948.1"
                     /translation="MYAFVAPTALTRCRHRVSAMKFRIVTFNLEQNHKKWSQRRRLIV
                     EQLAQLNPDMLALNEIHIPEQTGRWLRAAAGEQLDRPYRLLQQSKAGAEGRVQGEGLL
                     TRYCPIETANLDYNSHDCIAMAARCEIGDRLIDVYVTHLIAARIDESARQYQVEHLLQ
                     WIAGRDDADGAIVCGDFNATPDQPSTRLMSGTFQATQFAPTAFTPLRDSTGKPTHPEW
                     PRFDRCIDYIWFKGALRLCASGRCFDKPADDDSDLWPSDHLGVWADFETC"
     gene            1241..2239
                     /locus_tag="VE170_00560"
     CDS             1241..2239
                     /locus_tag="VE170_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13949.1"
                     /translation="MKPCRALQIVAILAGIILNRHNALALDKVIIGFSSLSAVHGATW
                     VAEEKGLFKKHAIEPQIVIMPGTAVGISALMAGDVDFLNAGGNIVIGANLKGADLIML
                     GAYINKGVQSVMARSDIKTPGDLKGKTVGVTRLFDGGHRLLERALSIWKINPADVRLL
                     ALGSSPAMLARLEKGGIDAAVLTIPSVFVAEDKGFRVLANFSDMGLYSLSGMLSTRRA
                     FLRANRDLATRFMKGFVEGIAYFNRNKKESVAVLRKKLRTGAEGEKYLERAYDLLANK
                     QYERVPTPSPLGLKTVMEIVALDDPKAKTVDPTAFIDGSIIKELDDSGFIKSLYER"
     gene            2278..3357
                     /locus_tag="VE170_00565"
     CDS             2278..3357
                     /locus_tag="VE170_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ13950.1"
                     /translation="MSSASNGSQKANEVLISSDSHVIEPAELLKQRVATAFRDRAPLF
                     KHQALGGGFQGHPGGGDPNHRIKEMETDGLSAEVLYPTYILDLFAMDDAQLQAACFRA
                     YNDWLMEYCQVAPQRLFGIAAISVYDIEDAVQELERCKKAGMKGSIIWQVPHPKLPFS
                     SDHYDKFWAASQDLDMPVSLHILTGYGYHMSEAHLTTVERYRGSVITKYQEIAGALFE
                     LMFYGAFERYPGLKFVTVENEVGWIPFTLQQWDYYYGRHYKMTPSPIKSQVPSEVFGR
                     HVFSTFFRDQVGGHNFEWWGSDNCMWSTDYPHGNSTWPNSREIMERNLTRLSPEQKYK
                     LVCGNVAKLYDIAVPQSAGKIAAGN"
     gene            3417..4481
                     /locus_tag="VE170_00570"
     CDS             3417..4481
                     /locus_tag="VE170_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ13951.1"
                     /translation="MKKRSLAGAYFIMLLVALNVASISTGHAQSESFYKGKTLKVLVG
                     TTAGSFYDIWARTVAAHLPRHLPGNPDALVQNMPGAGHKIATNYMYNVAKPDGLTIVG
                     SILPGVYMDQLAGRPEVKYDWAKFVWIGSPVKSESQMTMRADAPYKSIDDIRKAKEPP
                     RCGSTGTSGTDYMLARLLEEIMPPLKIHSVLGYPGGPELDLGIERGEIQCRTFTIETF
                     FAREPYITWKKKGFVRNLYQTGRNRDPRMADVPTLYEIMDGYKIPESGRRLATVILAN
                     GTLGRPIFTSPGTPTDRVKMLREAFGKMVKDPAFLEDAKKKNFELDPISGEELEVIVK
                     EVMTQPAETIARLKTILTQP"
     gene            complement(4515..4727)
                     /locus_tag="VE170_00575"
     CDS             complement(4515..4727)
                     /locus_tag="VE170_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13952.1"
                     /translation="MKPAISAFLIVLFFSFMGCTAAPDRILMKEQGEDNRCHMKIETY
                     GDPLMPTEREVVDFYGPCDERPGPRR"
BASE COUNT         1147 a         1321 c         1321 g         1045 t
ORIGIN      
        1 cgataaacca aaaataaaag tccgcgctga acagcgaatg acctacgaaa aactataggt
       61 gccgcgatgc ataccgcgaa tttgctgtcg gcttagccga cctcgcgtgt atgcattcgt
      121 cgcgcctact gccttaacca ggtgtaggca ccgtgtgagc gcgatgaagt ttcgcatcgt
      181 aacgtttaat ctggaacaaa accataaaaa gtggagtcag cggcgccggt tgatcgttga
      241 gcagttggcg caattgaatc ctgacatgct agcgctcaac gagattcaca ttccggagca
      301 aaccggccgc tggctgcggg cggcggcggg cgaacaactg gatcggccat accgactgtt
      361 gcagcagtca aaagctggag cagagggccg cgttcagggt gaagggctac tgacccgtta
      421 ctgtcccatt gaaacggcca atctcgacta taattctcac gactgcatag cgatggcagc
      481 ccgttgtgaa atcggtgatc gcctgattga tgtgtatgtc acccatctga tcgccgcgcg
      541 gatcgacgag tcggcgcgcc aataccaagt cgagcatcta ctacagtgga tcgccggccg
      601 tgatgacgcc gatggcgcca tcgtctgcgg cgatttcaac gccacgccgg atcaaccgtc
      661 gactcgactg atgtccggaa cttttcaggc gacccaattc gccccgacgg cttttacgcc
      721 tctgcgcgat tcaacaggca aacccacgca tcccgagtgg ccacgattcg atcgttgtat
      781 agactacatt tggtttaaag gggctttaag actttgcgcc tcagggcggt gcttcgacaa
      841 gccggccgac gacgattcag atctttggcc gtcggaccat ctcggtgtct gggcggactt
      901 cgagacctgc tgattccatc caaaaaattc ctccgcggtt atattcagcc tgagctagcg
      961 cgtttacaat cgccggtgat tgcggttcgt atctgcatac ggattcagga cagcggcgct
     1021 cgcgtttgcg aataagccgt cgcccacttt tggctaagct catcatccca cagaacttcg
     1081 aaacgctctc gaacacgagg cgtggatttc aaccctccta gtttctctga tgcttgacac
     1141 tttgttggac caaggactac aagtgaattt cgaagaaatc tcagatttag atctcaagac
     1201 gtgcgcgcat taccagcatg cggtgagcaa aggacccggc atgaaaccat gtagggcgct
     1261 gcaaatagta gcaatactcg cgggtataat tcttaatcgc cacaacgcgc tcgctttaga
     1321 taaggtgatt atcggttttt cttcgttaag tgcagtgcat ggcgcaacct gggttgctga
     1381 agaaaaaggg ttattcaaaa aacacgctat cgaaccacag atagtcatca tgccgggcac
     1441 tgccgtcggt atcagcgctc tgatggcagg agatgtggat ttcctcaacg ccggcggcaa
     1501 tatcgttatc ggcgccaatc ttaagggcgc cgacttgatt atgctcggtg cctacatcaa
     1561 caaaggcgtg caaagcgtca tggcgcgcag cgatatcaag acccctggcg acctcaaagg
     1621 taagaccgtc ggggtcacgc gcctgtttga cggcggtcac cggctcttgg agcgcgcctt
     1681 gagcatctgg aaaatcaatc cggcggacgt ccgcttgcta gcattggggt cgtcgccggc
     1741 gatgctcgcg aggctggaga aaggcggcat cgatgcggcg gtgctgacga taccgtcggt
     1801 ctttgtcgcc gaagacaagg gctttcgagt tctggcaaat ttttccgaca tgggccttta
     1861 ttccctcagc ggcatgctgt caacgcggcg ggcgtttctg cgcgctaacc gcgatctggc
     1921 aacccgcttc atgaaaggtt ttgtcgaagg catcgcctac ttcaatcgaa acaaaaaaga
     1981 gagcgtggcg gttttacgaa aaaaactccg gaccggtgcc gaaggtgaaa aatatctcga
     2041 acgcgcctac gatcttctcg ccaacaagca gtacgaaagg gtgccgactc cttccccatt
     2101 ggggttgaaa accgtcatgg agattgtcgc gttggacgat cctaaagcta agaccgtcga
     2161 tcccactgcc tttatcgatg gctcgattat caaggagttg gacgacagcg gatttatcaa
     2221 gagtctatat gaaagataag cataggcctc tgcgcctgcg gccaaggaga acccggtatg
     2281 tcaagtgcaa gtaatggaag tcagaaagcc aacgaggttc taatttcctc ggactcccac
     2341 gtcatcgaac ccgccgagct cttgaagcaa cgggttgcga cggccttccg cgaccgcgcg
     2401 ccgctgttca aacatcaggc cctcggcggt ggctttcagg gtcatcccgg cggtggcgat
     2461 cctaatcacc gcatcaaaga aatggagacc gacggcctga gcgccgaagt gctttacccc
     2521 acttatatcc tcgacctgtt cgccatggac gatgctcaac ttcaagccgc ctgctttcgc
     2581 gcctacaacg actggctgat ggagtattgc caagtggcgc cgcaacgttt gttcggtatc
     2641 gcggcaattt cagtctatga catcgaggac gcggtgcaag aactggagcg ctgcaaaaaa
     2701 gccggcatga aaggctcgat catctggcag gtgccgcacc cgaagctgcc gtttagctcg
     2761 gatcattacg ataaattctg ggccgcctca caggatctcg acatgccagt cagcctgcac
     2821 attcttaccg gatatggtta tcatatgagc gaggcacacc tgaccaccgt cgagcgttat
     2881 cgcggcagcg ttatcaccaa ataccaagag attgccggcg cgcttttcga attgatgttt
     2941 tatggcgcct tcgagcgtta tccgggcttg aaattcgtca cggtggaaaa cgaagttggc
     3001 tggattcctt tcacgttgca gcaatgggat tactattacg gccggcacta taaaatgacg
     3061 ccgtcgccga tcaagagcca agttcccagc gaagtttttg gccgtcacgt cttctcgact
     3121 tttttccgtg accaggtcgg cggtcacaac ttcgaatggt ggggctcgga taattgcatg
     3181 tggtcgacgg attatccgca cggcaactcg acctggccca attcccgcga aatcatggaa
     3241 cgcaacctga cgcgtctttc gcccgagcaa aaatacaaac tcgtgtgcgg caacgtggcc
     3301 aagctttatg atatcgcagt gccccagagc gcgggtaaaa tcgccgcagg caattaagca
     3361 tggtgcgata catggacact tgacggtgag tggatacgga cagagcagga ggcggaatga
     3421 aaaagcggtc tctcgccggc gcgtatttta tcatgctcct ggttgctcta aacgtcgctt
     3481 cgatttccac gggccacgcg caaagcgagt ccttctataa gggaaagacc ctcaaagttc
     3541 tggtcggaac cactgcgggc tctttttatg atatttgggc gcgcacggtt gccgcccatt
     3601 taccgaggca cctcccaggc aatccggatg cgctcgttca gaacatgcct ggagccggcc
     3661 acaagattgc caccaattac atgtataacg tggccaagcc cgacggcctg acgatcgtcg
     3721 gatccatttt accgggagtg tacatggacc aactcgccgg gcggccggag gtcaaatatg
     3781 actgggcaaa gtttgtctgg attggctcgc cggtcaagag tgaatcccag atgaccatgc
     3841 gcgcggacgc gccctacaaa tccatcgacg acatacgcaa ggcgaaagag ccaccgcgtt
     3901 gcggcagcac cggcacttcc ggcacggact acatgctggc gcggcttctc gaagaaatta
     3961 tgccgcctct caagatccac tccgtgctgg gatatccggg cgggccggag ctcgatctcg
     4021 gcattgagcg aggcgaaata cagtgccgga cctttacgat cgaaacgttt tttgcgcgcg
     4081 agccttatat tacctggaaa aagaagggct tcgttcgcaa cctctatcaa acaggacgga
     4141 accgcgatcc gcgcatggcc gatgtgccga cgctgtatga gatcatggac ggttataaga
     4201 tacccgagag cggccggcgc ctggcaaccg tcatcctcgc caacggcacg ctgggcagac
     4261 cgatattcac ttcgcccggg acgccgacgg atcgggtaaa gatgctgcgc gaagcctttg
     4321 gaaagatggt caaggacccg gcttttctgg aagacgccaa gaaaaaaaac ttcgagcttg
     4381 acccgatcag cggcgaagag ctggaagtca tcgtcaaaga ggtcatgaca caaccggcgg
     4441 agaccatcgc ccgcttaaaa acaattctga cgcaaccgta agattgattg cgcggaatag
     4501 ctacggcgtc acgcttaacg ccgggggcca ggccgttcat cacagggtcc gtaaaaatcg
     4561 acaacctcgc gttcggtcgg cattaatgga tcgccatagg tctcgatctt catatggcag
     4621 cgattatcct cgccctgttc tttcatgaga atgcggtcgg gtgcggcggt gcatcccatg
     4681 aacgaaaaga ataacacaat aagaaacgcg gatatggccg gtttcatttt aacttccttc
     4741 cctggttcac tgacctagta ggcttacagt cccttcctat tcaacgtaac gctttagggc
     4801 gccaagggct agaggaaatc gatcgaggta attg
//
LOCUS       DASQKS010000026         5735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4214285, whole genome shotgun sequence.
ACCESSION   DASQKS010000026 DASQKS010000000
VERSION     DASQKS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214285"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VE170_00580"
     CDS             complement(<1..428)
                     /locus_tag="VE170_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13953.1"
                     /translation="MTLRSWKLSQGIAALLLLVCCDITWVAAQASFFDGKTIRLLVGY
                     PAGTTHDIWVRTVTPYLKKYLPGNPDFIVQSMAGAGSMVAVNYLNGVAKPDGLTLATF
                     NAALYFEQLIGRKEVLFDWPKLAWIGSSTPATRLFYIRAD"
     gene            complement(513..1496)
                     /locus_tag="VE170_00585"
     CDS             complement(513..1496)
                     /locus_tag="VE170_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ13954.1"
                     /translation="MKTKSLILTAIALFCAAELALAADKLRVAYVSPSVSQSLPWIAK
                     ELGILAKHDLAVENLLITGSPRLVQALIAGDVDVIFAGVTAMTRARMRGADVAILGAS
                     ANLSSQKLMVSRTSKIRRLEEVKGAVIGVSQYGSEADTFARNALAKVGLKPDKDVTII
                     QLGGHPQVAAALAAGKLEAGILGGLAILTAERSGARFITSAADLKILSPSGTFATTRG
                     YIQRKRSVVERLTRSFVEAIHYLKTNRSGAIVLLQRYLGGLSADEASYLYDEQVEFLE
                     RIPGPNEKALAAVLERESDPKVKSLAIGDFIDPSFFREIEKSGLIEQLYRK"
     gene            complement(1515..2522)
                     /locus_tag="VE170_00590"
     CDS             complement(1515..2522)
                     /locus_tag="VE170_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ13955.1"
                     /translation="MIPCVIALVSLIAVSLPASAQTLAQIVEGAKTEKELLTYATTQA
                     PVMNRLVQGFNKKYPFVKVEYYRAGAEKIAQRFDTEAAAGRHVVDVVNLSGSEMYWTA
                     KKGNFAVYDSPERKAIRPSYKDKQGLWTAQYSTIAVIGYNRKLVAAADVPKSYDDLLL
                     AKWKGHIGLDQTDDDWYMVLVHVMGEKKAAEFIRALAKQNVEFRRGRQLAAELLAAGE
                     WAMAPTMRIAAGEEIRRKGAPVDWVAMEPLFPNPPNALALARRAPHPNAGKLWIDYIL
                     SKEVQDYYANEVGVSVGRADITLANERLRKLRYGEIDWPVYLEKLQHYQDEWRKVMKP
                     Y"
     gene            2708..2872
                     /locus_tag="VE170_00595"
     CDS             2708..2872
                     /locus_tag="VE170_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13956.1"
                     /translation="MAIEEFAHAAQTITDVTTERVEGIYKLILARAMRQDGDLISAKL
                     LKLATQNRRL"
     gene            2951..4156
                     /locus_tag="VE170_00600"
     CDS             2951..4156
                     /locus_tag="VE170_00600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ13957.1"
                     /translation="MATPTGPLTGYRVLDLGGPLSLHCTKLLADMGADVIKIEAPGGD
                     ESRRVPPFKDDQAHPEKSLYFLHFNTNKRGITLDVEKPDGRAILLELCKKADVIIETY
                     RPSRAKELALTYQDLSAVNPALIVASITPFGQNGPWKDYKANDMAGIALGNLLYLAGE
                     PGEPPLQPPGEIAYGMASTYGAFGIAVALYHRLDSGKGQHIDVSMHECGGHIAGYFIP
                     NYGYTGDKPARASRKGEETDLYDPYKTKNGYARIFIIPVEQWRRLVEWMGSPPSISGP
                     EFEKMAYRRKHPEIVVGAVAEFCARYTKEELYEEGQKRRIAVTPINTAGEFVEMEQTK
                     ARGLFVDMEHPVVGKYKQFGPVPLLMETPGGIFRPAPLLGEHNRDIYIGDPGMTTDDL
                     VALQAEGVI"
     gene            4268..5476
                     /locus_tag="VE170_00605"
     CDS             4268..5476
                     /locus_tag="VE170_00605"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ13958.1"
                     /translation="MSQLPLEGIRVIELTTGAAGPTVARVLCEFGAEVIRCETRLRGD
                     GHRGEDPKLWNKKPDFMKLQRGKKSFTVNMQTAKGRDLVKELVKKSDVLVENFGLGIL
                     EKWGLNWPDLQMINPRMILIRVKGMGCSGPHATDLTYGPNVGNTMATTYLWNYPGATT
                     ATAEPRTQHPDFMGGVTGAFGVVLALMERKKTGRGQWIDNAQQEIGAALLGPRYLEYT
                     VNKREPQPEGNRSLVASPYGPYQCKGNDRWCVIAAYTQDEWERFAGLVEKSGVKRSAN
                     FATHWQRVRHKDELDKWVTDWTLQHDPYEVMELVQGVDVCAAVVQDVEDQFKNDKQYA
                     ATGFLVNMTEPEAGDVVTENVPMRLSLTPGKVRGVAPLMGEHTHEIARNLLGLSDDQI
                     KKLDEEKVLY"
BASE COUNT         1310 a         1718 c         1594 g         1113 t
ORIGIN      
        1 tgatcggcgc ggatatagaa aagccgcgta gccggcgtcg aactgccgat ccaggcgagc
       61 ttgggccagt cgaacagtac ttccttgcgg ccgatcaact gttcgaaata gagcgcggcg
      121 ttaaaagtcg ccagtgtcag gccatccggc ttagcgacgc cgttgagata attcaccgcc
      181 accatggagc ccgcgccggc catcgactga acgatgaaat cgggattgcc ggggagatat
      241 tttttcaggt agggtgtaac cgttcggacc cagatgtcat gggtggtgcc ggcgggataa
      301 ccgacgagca atcggatcgt cttgccgtcg aagaacgacg cttgtgcggc gacccaggta
      361 atgtcgcagc agaccagcag aagcaaggcc gcaatcccct gcgaaagttt ccagctgcgt
      421 agagtcataa agcatcgact cccttcggta atgcgacggc ttgtcctgtg tccctgtgcc
      481 gacagacccg caacttctcc agatcgacaa acctacttcc gatacagttg ctcgatcaat
      541 ccgctctttt caatctcgcg aaaaaaactt gggtcgataa aatccccgat ggccaggctc
      601 ttgaccttgg gatcgctctc ccgttcgagc accgcggcga gcgctttctc attggggccg
      661 ggaatgcgct ccaaaaactc gacctgttcg tcatagagat aactcgcctc gtcggcgctc
      721 aagccgccga gatatcgttg cagtaagaca atcgcgccgc tgcgattggt cttcaaatag
      781 tgaatcgcct cgacaaagga gcgggtcagc cgctcgacga cgctgcgctt gcgctgaata
      841 tagccgcgcg tggtggcgaa ggtgcccgag ggcgagagga ttttcagatc ggcggcgctg
      901 gtgatgaagc gcgcgcccga cctctcagcg gtgagaattg ccaaaccgcc gagtattccc
      961 gcctcgagtt tccccgccgc caaggcggcg gcgacctggg ggtggccgcc gagctgaatg
     1021 atcgtcacgt ctttgtccgg cttcaagcct actttggcca gagcattgcg cgcgaaggtg
     1081 tccgcctcgg aaccgtactg cgagacgcct atgaccgcgc ccttaacttc ttcgaggcgg
     1141 cgaattttcg atgtgcggct caccatcagt ttttgactgg aaagattcgc cgaagcgccg
     1201 agtatcgcca cgtccgcgcc gcgcatgcgc gctcgggtca tcgccgtcac gccggcgaaa
     1261 atcacatcga catcgccggc gatcagcgcc tggaccaaac gcgggctgcc ggtgatcaac
     1321 agattttcca cggccaaatc gtgcttggcg agaatgccca gctccttggc aatccaaggc
     1381 agggactgcg agacgctcgg actgacgtag gcgacccgca gcttatcggc ggcaagcgcc
     1441 aattccgcgg cgcaaaataa tgcaatagcg gtcaatatca aactttttgt tttcatcgtg
     1501 gatgatcgac taggttaata aggcttcatg actttgcgcc actcgtcctg ataatgctgc
     1561 agcttttcca aatacaccgg ccagtcgatc tcgccgtagc gcagcttgcg cagccgttcg
     1621 ttggccagag ttatgtcggc gcggccgacg ctgacgccga cttcgttggc gtaataatcc
     1681 tgaacttctt ttgaaagaat gtaatcgatc cacagcttgc cggcgttggg atggggcgcc
     1741 cggcgcgcca gcgccagcgc gttgggcgga ttggggaaaa gcggttccat ggcgacccaa
     1801 tccaccggcg cgcctttccg gcgaatctct tcgccggcgg cgatgcgcat ggtcggcgcc
     1861 atcgcccact cgccggcggc gagcagctcc gccgccagct ggcggccgcg gcgaaattcg
     1921 acattttgct tggccaaagc gcggatgaac tccgccgctt tcttctcgcc catcacatgc
     1981 accagcacca tgtaccaatc gtcgtcggtt tgatccaacc cgatgtggcc tttccatttt
     2041 gccaggagca gatcgtcata ggatttcggc acgtcggccg ccgccaccag cttgcggttg
     2101 tagccgatca ccgcgatggt cgagtactgc gccgtccaca aaccctgctt gtctttataa
     2161 cttgggcgaa tcgcttttct ttccggcgaa tcgtaaaccg cgaagttgcc tttcttggcc
     2221 gtccagtaca tctccgagcc gctcaagttg acgacatcga caacgtggcg tcccgcggca
     2281 gcctcggtgt cgaaacgctg ggcaatcttt tccgcgccgg cgcgatagta ctcgactttg
     2341 acaaacggat attttttatt gaacccctgc accaagcgat tcatcaccgg cgcctgggtg
     2401 gtcgcgtagg tgagcagctc cttttccgtt ttggcccctt cgacgatctg cgccagcgtc
     2461 tgggccgaag ccggcaatga aactgcaatc aacgacacca aagcgatgac acacgggatc
     2521 atggttcact ctccatgatt gcgatactaa tcaagccggc gacgatttgt aaacctaaaa
     2581 acatttcacc gcgaagaaca gaacctgccg aagccgcgac tgaatagaag aatggccgca
     2641 aagaacgcaa ggggtgcaag aaacattcgg cagttgaagg atcgggtttg aaaccgtcat
     2701 ctcggcggtg gccatcgagg aatttgccca cgccgcgcaa acgatcacgg atgttactac
     2761 cgagcgcgtc gaggggattt acaaattaat tcttgcgcgc gccatgcggc aagatggaga
     2821 tttaatttca gcaaaattgc taaaacttgc aacgcaaaac cggagacttt gaggtcgccg
     2881 ccttgacacc ctactttcat ccaagtaaga atctcggaaa atcaagttta atttcgctcc
     2941 gaggaatttc atggcaacac caacaggacc gttgaccggt taccgcgtgc tcgacctagg
     3001 cgggccgctc agtctgcact gcaccaagct cttggccgac atgggcgccg atgtgatcaa
     3061 aatcgaagcg cctggcggcg acgaatcgcg ccgcgtgccg ccgttcaaag acgatcaggc
     3121 gcatccggaa aagagtctgt actttcttca cttcaatacc aacaaacgcg gcattaccct
     3181 cgacgtcgaa aaacccgacg gccgggcgat actgctggaa ctgtgcaaaa aagccgatgt
     3241 gatcatcgaa acctatcggc cgtcgcgcgc caaggaattg gcgctgactt atcaggatct
     3301 gagcgcggtc aatccggcat tgatcgtcgc atcgatcacg cccttcggcc aaaacggccc
     3361 atggaaagac tacaaagcca acgacatggc cggcatcgcg ctgggcaact tgctctatct
     3421 cgccggcgaa ccgggagagc cgccgctgca gccgccgggt gagatcgctt acggcatggc
     3481 gtcgacctat ggcgccttcg gcatcgccgt cgcgctgtac caccgcctcg atagcggcaa
     3541 gggccagcat atcgacgtat cgatgcatga atgcggcggc catatcgccg gctattttat
     3601 tcccaactac ggttacaccg gcgacaaacc ggcgcgcgcg tcgcgcaagg gcgaagagac
     3661 cgacctctac gatccttaca aaaccaagaa cggctacgcg cgcattttta tcatacccgt
     3721 cgaacaatgg cgccgcctgg tcgagtggat gggcagcccg ccgtcgatct ccggcccgga
     3781 attcgaaaag atggcgtacc ggcgtaaaca cccggaaatc gtcgtcggcg ccgtcgccga
     3841 gttctgcgcg cgctatacca aggaagaact ttacgaagaa ggccagaagc gccgcatcgc
     3901 cgtcaccccg atcaatactg ccggcgaatt cgtcgagatg gaacagacca aggcgcgcgg
     3961 cttgtttgtc gacatggagc atccggtagt cggcaaatat aaacaattcg gcccggtgcc
     4021 gctgctgatg gaaacgcccg gcggcatctt ccgccccgcg ccgctgttgg gagagcataa
     4081 ccgggacatc tacatcggcg atcccggcat gaccacggat gacttggtcg ccctgcaagc
     4141 cgagggagtg atctgaaaaa gaattggaga gctggaggac tgaagtatcg gcgtgatcgg
     4201 ttccaattcc gaccaacact ctatttactc catcactcca accatttaac cagcaggtga
     4261 atattttatg agccaattac cccttgaagg cattcgtgtg atcgaactta ccaccggcgc
     4321 cgccggcccc accgtcgcgc gcgtgctctg tgaatttggc gcggaggtga tccgctgcga
     4381 gacccgtctg cgcggcgatg gccatcgcgg cgaagatccc aagctgtgga acaagaagcc
     4441 cgacttcatg aagctgcagc ggggcaagaa atctttcacg gtcaacatgc agactgccaa
     4501 gggccgcgat ctggtcaaag agttggtgaa aaagtccgac gtgctggtgg aaaacttcgg
     4561 gctgggcatt ttggaaaagt ggggcctcaa ttggcccgac cttcaaatga tcaatccgcg
     4621 catgatcttg atccgcgtca aaggcatggg ctgctccggg ccccacgcca ccgatctcac
     4681 ctacggtccc aacgtcggca acaccatggc gacgacttat ttgtggaact accccggcgc
     4741 gaccacggcc accgccgaac cgcgcaccca gcatcccgac tttatgggcg gcgtcaccgg
     4801 cgccttcggc gtcgtcctag ccctgatgga aagaaaaaag accggccgcg gccagtggat
     4861 cgacaacgcc cagcaggaga tcggcgccgc cctgctcgga ccgcgttatc tcgaatacac
     4921 cgtcaacaag cgcgagcccc agcccgaagg caaccgcagt ctcgtcgcct cgccctacgg
     4981 accctaccaa tgcaaaggca atgatcgctg gtgcgtgatc gccgcctata cgcaggacga
     5041 gtgggaacgc tttgccggcc tagtggaaaa atcaggggtc aagcgcagcg ccaatttcgc
     5101 cacccactgg caacgggtgc gccacaaaga cgagctcgac aagtgggtga ccgactggac
     5161 gctgcagcac gatccctacg aagtaatgga actggtccaa ggcgtcgacg tctgtgccgc
     5221 cgtggtgcag gatgtcgaag accagttcaa gaacgacaag caatacgccg ccaccggttt
     5281 tctcgtcaac atgaccgagc ccgaggccgg cgatgtcgtc actgaaaacg tcccgatgcg
     5341 cctgtcgttg acgccgggaa aagtccgcgg tgtcgccccg ctcatgggcg agcataccca
     5401 cgagatcgct cgtaacttgc tcggtctcag cgacgaccaa atcaaaaaat tggacgaaga
     5461 gaaggtgctc tattaacaat tgaaaattaa aaattgagaa ttgaaaacga atccgcttaa
     5521 gcttagcacc gctccgacat tttcaatttt ccattatcaa ttttccactt ccaagaggag
     5581 gaacacatgg ccgaagcagg acccctatgt atttacgaga agcgcgatcc ccatatcgcg
     5641 atcatcaagc tcaatcgccc ggagaaaaaa aatgccatca gccgcgacct ttactgggcg
     5701 ctggatgaat gttggcagaa agctaaagcc gacga
//
LOCUS       DASQKS010000027         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3793835, whole genome shotgun sequence.
ACCESSION   DASQKS010000027 DASQKS010000000
VERSION     DASQKS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3793835"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VE170_00610"
     CDS             complement(<1..1022)
                     /locus_tag="VE170_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HYJ13959.1"
                     /translation="MTDKPLEQTGLRDLRGIMRQRAPAEAWKHFNGAAETKATFHRNP
                     RAFQQYLFRQKIFHEVSEPDISIELFGKTLPIPAITAPVGSFSLIGKDAEREVAEGTD
                     RAGAMMLVSQAAHFNLHDWRAADKSPLVFMAYMNRGREEIEDYAKTAQDLGFAAVGIT
                     MDTVKPVKIGDEVPLSTKDGKPRKGHKSTPKDIEWMKQMVKLPVAVKGIMGADDAKIA
                     VNSGADAVVVSNHGGRILDFNRSALEALPEVVDAVGSKVPVLLDSGIRSGGDIVKAMA
                     LGAKAVLIGRPVAWGVGAFGARGVERVFNIFAEEMQRVLVMTGVGRVRDVTQEILVRD
                     DWSKAR"
     gene            complement(1120..1971)
                     /locus_tag="VE170_00615"
     CDS             complement(1120..1971)
                     /locus_tag="VE170_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ13960.1"
                     /translation="MIQTTALAATHMECRNLQESLKVLTGLLAFEKISERPGEATLKH
                     PNTHWQLVVHEAGPNAPAKPMHNHWGVRVVSPEEVDRAYDYLLAHKAEYKLGAIGKPT
                     WSHGSYSCYFVEPGSNGWEIECYEAFNRKQATAERFGAVKMAHWDQLLPEERFPGRGY
                     LPQGFTHGTLVSTDLEASKNFYTDVLGLDAHRFSDNVIYIKHPTTKTFIVCALRQNAP
                     VFSANFRNTLTVASKDAVKAAYREFSESGRALGVSELFALEETDTSASFCFRDPGTNC
                     WEITMAH"
     gene            2229..>2758
                     /locus_tag="VE170_00620"
     CDS             2229..>2758
                     /locus_tag="VE170_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13961.1"
                     /translation="MKLPIALVTAALLTRAILPGNAAAQENFYQGKTIRMIVGTSAGG
                     GFDTYTRTLARHFGKHVPGQPTIVVENMAGAGHLIAANHMYRVAKPDGLTLGHFHGGW
                     FLYQLFKRPGIEFDATKYEFLGSPIKESRACALTKASGITSVEKWLASKAPVKIGGIG
                     GGAPDDMARMLAATTAL"
BASE COUNT          579 a          879 c          726 g          574 t
ORIGIN      
        1 taacgcgcct tgctccagtc gtcgcgcacg agaatctcct gggtcacgtc acgcacccgg
       61 ccgaccccgg tcatcaccag cacccgctgc atctcttcgg cgaaaatatt aaacacccgc
      121 tcgacgccac gcgcgccaaa agcgccgacg ccccaagcca ccggccggcc aatgagcacc
      181 gctttggcgc ccaacgccat cgccttgacg atgtcgccgc cgctccgaat gccactgtcg
      241 agaagaacgg ggactttaga gccgacggca tcgacgactt ccggcaacgc ttccaacgcc
      301 gaacgattga agtcgaggat ccgcccgcca tggttggaga ccaccacggc atcggcgccg
      361 gaattgacgg caatcttggc gtcatccgcg cccatgatgc ctttgaccgc caccggcagt
      421 ttaaccatct gcttcatcca ctcgatgtct ttgggcgtcg acttatggcc cttgcgcggt
      481 ttaccgtcct tggtcgacaa cggcacctca tcgccgatct tcaccggctt gacggtgtcc
      541 atcgtgatgc cgacagcggc gaatcccaaa tcctgtgccg tcttggcata gtcttcgatc
      601 tcttccctac cgcggttcat gtaggccatg aataccagcg gcgacttgtc ggcagcgcgc
      661 cagtcgtgca ggttgaaatg cgctgcctga ctgaccaaca tcatcgcgcc cgcgcgatcc
      721 gttccctcag ccacttcgcg ctcggcatct ttgccaatca agctaaaact ccccactggc
      781 gccgtgatcg ccgggatcgg caatgtctta ccgaacagct cgatggaaat atccggctcg
      841 ctgacttcgt gaaaaatttt ctggcgaaat aaatactgct gaaatgcccg cggattgcgg
      901 tgaaacgtcg ccttggtttc cgccgcgccg ttgaaatgct tccatgcctc cgccggcgcc
      961 cgctgacgca tgatcccgcg cagatcgcgc agccccgtct gttccagtgg tttgtcagtc
     1021 ataatttatc cgcctatctg tgcttttgtt gcagcaaccg caagggcgac cggcaccctc
     1081 acaaccatat taaagggaga gaaggttcgc gtcggcaaat caatgcgcca tagtaatttc
     1141 ccaacagttt gtccccggat cgcggaaaca aaaggacgcg gaggtatcgg tctcttcaag
     1201 cgcgaataac tcgctcacgc ctaaagccct gccggattcg ctgaattccc gataggcagc
     1261 tttcaccgca tccttggacg cgacagttaa ggtattgcga aaattcgccg agaacaccgg
     1321 ggcattttgg cgcagggcgc agacgataaa agttttggtc gtcggatgct tgatgtaaat
     1381 cacattatcg ctgaagcggt gcgcgtccag gcccaagaca tcggtgtaaa agttcttgct
     1441 cgcctccagg tcagtggaaa ccaaagtgcc atgggtaaac ccctggggca ggtagccgcg
     1501 ccctggaaag cgctcctccg gcaacaattg atcccagtgc gccatcttca ccgcgccgaa
     1561 gcgctcggcg gtcgcctgct tacggttgaa agcctcgtag cactcgatct cccagccatt
     1621 gctgccgggt tcgacgaaat agcaggaata agagccatga ctccaggtcg gcttgccgat
     1681 ggcgccgagt ttgtattccg ctttgtgcgc cagtaaataa tcataagccc ggtcgacttc
     1741 ctccggactg accacgcgca ccccccaatg attgtgcatc ggctttgccg gcgcgttcgg
     1801 cccggcttca tgaaccacca actgccagtg agtgttagga tgcttgagcg tcgcctcccc
     1861 cggtctctcg gatatttttt caaaggccaa cagcccggta agcactttca acgactcttg
     1921 caagttgcgg cactccatat gtgttgccgc aagtgctgta gtttgaatca tcttgaatct
     1981 cctaacgatg actggatcat tgccggaaaa tttacagccg ctgcaccagg gtgtcaaact
     2041 gacccatgga ggtagctttc cgtgtggatg acggagcctt gaggtctgtg atctcgagcg
     2101 cagcgcgaac tctctcccaa tcgcagcacc ggaccaacat gaaacccgat cagcggcaat
     2161 ccgccttgac acaaacttgc cttttccctt acattcgccg agccttagac ggaaggagcg
     2221 gactctcgat gaaattgcca atcgcgcttg tcacggcggc gctgttaacc cgcgccatcc
     2281 ttcctggcaa tgctgccgcc caggaaaatt tttaccaggg caagaccatc cgcatgatcg
     2341 tcggcacctc ggccggcggc ggcttcgata cctacacgcg caccttggcg cgccacttcg
     2401 gcaagcatgt gccggggcag ccgaccatcg tcgtcgagaa catggctggc gccggccatt
     2461 tgatcgccgc caatcatatg tacagagtcg ccaagcccga cggcctgacc cttggccatt
     2521 tccacggcgg ctggtttctc tatcaactgt tcaaacgacc gggcatcgaa ttcgacgcga
     2581 ccaagtacga attcttgggc tcgccgatca aagaaagccg cgcctgtgcg ttaaccaaag
     2641 ccagtggtat caccagtgtc gaaaaatggt tggcttcaaa ggcgcctgta aagatcggcg
     2701 gcatcggcgg cggcgcgccg gacgacatgg cgcgcatgct cgccgccacc acggcgct
//
LOCUS       DASQKS010000028         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1275571, whole genome shotgun sequence.
ACCESSION   DASQKS010000028 DASQKS010000000
VERSION     DASQKS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275571"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VE170_00625"
     CDS             complement(<1..428)
                     /locus_tag="VE170_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HYJ13962.1"
                     /translation="MNLSTDDTARATLSANIKAAAQKLGFALAGISPVRLPPHGESFA
                     RWLRDGFAGELGYMSRTEALRRDPHELVPWARSVISVGMNYYTPSARPETVDNSEGWI
                     SRYAWGDDYHDIIMARLEQLLETIRALCGESIEGRAFVDSG"
     gene            complement(675..1118)
                     /locus_tag="VE170_00630"
     CDS             complement(675..1118)
                     /locus_tag="VE170_00630"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HYJ13963.1"
                     /translation="MDVNFPRLFTVNEANGLLPVLRPLVEQILENIRRLKGVSETVIR
                     REQLDPDAPNLMERLREDDEVLKLVTQIKDWVTTINGHGCVCKGVEQGLIDFPCMLGA
                     EVVFLCWQIGEANVAHYHRIEDGFAGRKTLLDGGNAEPDSKISYH"
     gene            complement(1174..2469)
                     /gene="lpxC"
                     /locus_tag="VE170_00635"
     CDS             complement(1174..2469)
                     /gene="lpxC"
                     /locus_tag="VE170_00635"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HYJ13964.1"
                     /translation="MEELAVNKTILVVDDEDDVRESVREVLRDEGYRVIDTGDGTKVL
                     DLIRQERPELVLLDIWMPQVDGIGLLKEIKCQEPDLNVVMISGHGNIHTAVTATKFGA
                     FDFLEKPVSLDGLLLTVQRALGESSNTLVQQRRAKKKTAAAKGGEGPSAATASSNVKQ
                     RTLKKSVVLSGQGLHSGVKTGLILHPLPPNSGIRFTGISGEVTVPAHLDYVGSTGYAT
                     SLRTHGFAVGTVEHLLAVLHCYGIGNLLIKVQGEVPILDGSALEFCQAIDEAGMQQED
                     EETAEIVIDRAYRVEAKGGESIVIEPADSFSVRYDLRYPSPVGEQQYVYYHRGPASFR
                     EEIAPARTFGFLKDIAKLQTMGLANGGRLSNFILIDDEKIVNTELRFADELARHKILD
                     ILGDFFLLGRPFRGAIHGRMTGHSDNIALLRKIREGMKL"
     gene            complement(2482..>3568)
                     /locus_tag="VE170_00640"
     CDS             complement(2482..>3568)
                     /locus_tag="VE170_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ13965.1"
                     /translation="GDLKQITLELERRGKVVQTLLANIAAGVVSVDPAGNITTWNKAA
                     EQMLGVKTDQALAKNYREVLQPESLRVVAEIIDSVKDLEKIERNIKIPLSDRQLTAVV
                     TAATLRDDDGSVLGVMVFLEDITQIQKVERMEAWREVARRIAHEIKNPLTPIQLSAER
                     LRKRYASLLGGDGGILDKCTTTIIQQVEALKSLVNEFSQFARLPSAKLVTQDLNEIVS
                     ESLFLFKEGHKGIQFNFRSGVIPTLDLDRDQIKRVLINLLDNAVAAVQDDGEINLSTL
                     YDRGRDVVTLEVVDNGTGLAPEMRSKIFEPYFSTKENGTGLGLTIVSQIIEDHRGYIR
                     ALPNEPRGTRFSIEFPASEVAKRAVHS"
BASE COUNT          731 a         1077 c          979 g          781 t
ORIGIN      
        1 ccggaatcga caaaggcgcg cccttctatg gattcgccgc acagcgcgcg aatggtttcg
       61 agcagctgct ccaatcgcgc catgataatg tcgtgataat catcgcccca ggcgtagcgt
      121 gaaatccagc cctcggagtt gtccaccgtc tccggccggg cagacggcgt atagtaattc
      181 atgccgacgg aaataaccga ccgtgcccaa ggcaccagct cgtgcgggtc gcggcgcagc
      241 gcttcggttc ggctcatata gcctagctcg ccggcaaagc cgtcacgcag ccagcgcgcg
      301 aaggattcgc catggggggg caaccgaacc ggtgagatgc cggcgagcgc gaaccccagc
      361 ttctgcgccg ccgctttgat attagcggac agcgttgcac gcgcagtgtc gtcggtcgac
      421 aagttcatga atgggtcagc aatgagtgcg gccttgttgg taacgaccat cgcctcgggg
      481 gccgcagccg gaaccgcaac tcgagtttat cgtccgcggc tactccaagg ggcagtcgtc
      541 ccggacgaac gggaagaaac taggaagatt tctgcctacg gtcgaaatga cattccctcg
      601 aacttgctac cgtcgcacga tattcgcgcc atccagaaat tcgctgcacc tgtaaatcat
      661 acggcaggta gcgctcagtg gtacgatatt ttgctatccg gctccgcgtt gccgccgtcg
      721 agcaacgtct tgcgcccggc aaagccgtct tcgattcgat gatagtgcgc cacgttggcc
      781 tcgccgatct gccagcagag aaacaccact tcggcgccga gcatacaggg aaagtcgatc
      841 aacccctgct cgacgccttt gcatacgcag ccatggccgt taatcgtcgt cacccaatcc
      901 tttatctggg tgacgagttt caagacctcg tcatcctcgc gcagccgttc catcaaatta
      961 ggcgcatcgg ggtcgagctg ctcgcgccgg atcaccgttt cgctgacgcc cttcaagcgc
     1021 cggatatttt ccaggatctg ctccaccagc ggccgcagca ccggcaagag tccgttggct
     1081 tcattgacgg tgaagagtct gggaaaattg acatccatgt ttggcttctt tcaaattgtc
     1141 gttgggctgg gagagataat aactccccgg cgcttacagc ttcatgccct cgcgaatttt
     1201 gcgcagcagc gcaatgttgt ccgagtggcc ggtcatgcgc ccgtgaatcg cgccgcgaaa
     1261 aggccggccg agcaaaaaga aatcgccgag gatatcgagg atcttatggc gcgccaattc
     1321 atcggcgaag cgcagttccg tgttaacgat tttctcgtcg tcgatcagga tgaaattgct
     1381 caatcgcccg ccgttggcca gtcccatggt ctgcagcttg gcaatgtctt tgagaaagcc
     1441 gaaggtccgc gccggtgcga tctcctcgcg aaacgacgcc gggccgcgat gataatagac
     1501 atattgctgt tcgccgaccg gactgggata gcgcaggtcg tatcgcaccg agaagctatc
     1561 cgccggttcg atcacgatgg actcgccgcc cttggcttca acccgatagg cgcggtcgat
     1621 gacgatttcg gcggtttctt cgtcttcctg ctgcatgccg gcttcgtcga tcgcctggca
     1681 aaactccagc gctgagccgt cgaggatcgg cacttcgccc tgaactttga tgagcagatt
     1741 accgatccca tagcagtgta ataccgcgag caagtgctcg acggtgccga cggcgaaacc
     1801 gtgagtccgc aacgaggtcg cgtaaccggt ggagccgaca tagtccaaat gcgccggcac
     1861 cgtgacttcg ccggaaatac cggtaaagcg aataccgctg ttgggcggca acggatgcaa
     1921 aatcaagccg gtcttcacac ccgaatgaag cccttggccg gatagcacga cgcttttttt
     1981 caaggtccgt tgcttgacgt tgctcgacgc cgtggctgcc gagggccctt cgccgccctt
     2041 agccgcggca gtttttttct tggctcggcg ctgctggacc agggtattcg aggactcacc
     2101 caaagcgcgc tgcacggtca agagcagacc gtccaaggaa accggctttt cgaggaaatc
     2161 gaaggcgccg aatttggtcg cggtcaccgc tgtgtgaata ttgccatgac cggaaatcat
     2221 tacaacattg aggtccggct cctgacactt gatttctttg agcaagccga tgccgtcgac
     2281 ctgcggcatc cagatgtcca ggagaaccag ctctggccgc tcttgccgaa tcaagtcgag
     2341 cactttggtc ccgtcgccgg tatcgatgac gcggtaccct tcgtcgcgca gcacttcgcg
     2401 caccgactcg cgtacatcgt cttcgtcgtc gacgactaag attgttttgt taaccgccag
     2461 ctcttccact catcctccaa gttacgaatg tacggcccgt ttagcaactt ccgaggcggg
     2521 gaattcaatg ctaaagcgcg ttcccctcgg ttcgttgggt aacgcccgaa tatatccccg
     2581 atgatcctcg ataatttgac tgacgatggt caggcccagc ccggtgccat tttctttggt
     2641 ggaaaaatat ggctcgaata tttttgaccg catctccggc gccaaaccag tgccgttatc
     2701 gacgacttcc aacgtcacca cgtcccgacc gcgatcgtaa agagtcgata ggttgatctc
     2761 accgtcatct tgcaccgcgg ccaccgcgtt gtcgagcaga ttgatcagca ctcgcttgat
     2821 ctggtcgcgg tccaaatcta aagtcggaat cactccggag cgaaaattaa attgaatacc
     2881 tttgtgtcct tctttgaaaa gaaacagcga ttcgctgacg atctcgttaa gatcctgggt
     2941 cacgagctta gccgacggca gccgggcgaa ttgggaaaac tcattgacca ggcttttgag
     3001 ggcctcgacc tgctgaatga tcgtcgtcgt gcatttgtct aaaataccgc cgtcgccgcc
     3061 gagcagactg gcgtaacgct tgcgcagccg ctcggccgac agctggatcg gtgtcagggg
     3121 attcttgatc tcatgggcga tgcgccgcgc cacttcgcgc caggcttcca tgcgctccac
     3181 tttttgaatt tgcgtaatgt cttcgagaaa gaccatgact cccaacaccg agccgtcgtc
     3241 gtcacgcagg gtcgccgccg tcaccaccgc cgtcaattgc cggtcgctca gcgggatctt
     3301 gatattacgc tcgatcttct ccagatcctt gacgctgtcg atgatctcag ccaccacccg
     3361 gagcgattcc ggctgcaaga cctctcgata attcttcgcc aaggcctggt cggttttgac
     3421 cccaagcatt tgttcggccg ccttgttcca agtggtgata tttcccgccg ggtccaccga
     3481 cacgacaccg gcggcgatat tggccagcaa ggtttgcaca accttgccgc gccgttcgag
     3541 ttccaaggtg atctgcttca agtcgccc
//
LOCUS       DASQKS010000029         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4215665, whole genome shotgun sequence.
ACCESSION   DASQKS010000029 DASQKS010000000
VERSION     DASQKS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4215665"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VE170_00645"
     CDS             complement(<1..251)
                     /locus_tag="VE170_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13966.1"
                     /translation="MGQVIVRRALSGISILKATNPPCSSTCGISTRPKNRRPRFYRDI
                     EVSCRNLTTLFPLIKNDPTDVPAQAIFQSGYAMLLSENLL"
     gene            complement(485..664)
                     /locus_tag="VE170_00650"
     CDS             complement(485..664)
                     /locus_tag="VE170_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13967.1"
                     /translation="MNPWELIFRQSKVNLGGYAGDQLPPQNWHWPRGTWFQTRLHHLR
                     TDGGYFRALAHEQWQ"
     gene            complement(731..1129)
                     /locus_tag="VE170_00655"
     CDS             complement(731..1129)
                     /locus_tag="VE170_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13968.1"
                     /translation="MERTNTNTADAGNISVHRGEERINALFQPDTLLPAQYLDTMQRT
                     TFLEPEKRLMLAVLDDAVNCYRDNLFSQRGKDRRLFDEAEEWILTPGGDRAFSFDNVC
                     ESLGLNPEYLRRGLQRWKETHHKTELPSAA"
     gene            complement(1142..1480)
                     /gene="trxA"
                     /locus_tag="VE170_00660"
     CDS             complement(1142..1480)
                     /gene="trxA"
                     /locus_tag="VE170_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYJ13969.1"
                     /translation="MSEFVQNANDGNFTDAVLNTNKPVLVDFWAPWCAPCRALTPIVD
                     ALADQYAGAVQFMKVNVDDNPQAAQRYGVQAIPTLILFRGGEEKQRMVGVSRKEVIAQ
                     AIDLQVDQAN"
     gene            complement(1477..1752)
                     /locus_tag="VE170_00665"
     CDS             complement(1477..1752)
                     /locus_tag="VE170_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13970.1"
                     /translation="MLKITIDTEGEHANLELEGRLAGPWVQELRECWQRVANEHRQID
                     VVLKQVTFIDGAGRQLLAEMCRQGAVLAAEGCMTKAIVEEISGGEKP"
     gene            complement(1771..2178)
                     /locus_tag="VE170_00670"
     CDS             complement(1771..2178)
                     /locus_tag="VE170_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13971.1"
                     /translation="MSLEPKNYPRAAVRVIGGIILLSCIAAAGAQADDGGAQPPHARL
                     EAELAQVPFTLVSIAPLLGNGQTVGAIAIYDDRSTPRPVDYLEVFDGGGALVAVNWFD
                     RFGIERLIVDRALVEGRAELEGVFVAVVDGEVI"
     gene            complement(2885..3292)
                     /locus_tag="VE170_00675"
     CDS             complement(2885..3292)
                     /locus_tag="VE170_00675"
                     /inference="COORDINATES: protein motif:HMM:NF044909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ13972.1"
                     /translation="MAGDEQMVTQVFRDYVESFRSLKPDGVVPYCDAPCLFISAQGTR
                     MMKDTGEVTAFIAQLMEGLKARGFTRSELSELKVNQMSAHIAVVSVKRIRYKADGQEL
                     ERLGETYVLRKNAESWKIVMAMTHDADRVLQLA"
     gene            3267..3512
                     /locus_tag="VE170_00680"
     CDS             3267..3512
                     /locus_tag="VE170_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13973.1"
                     /translation="MTICSSPAIAKLLAKFAPSEADSTFAVSVISLERTVADAARVWV
                     LGIDPSKGSVTSNKQQNINAQLLALAYVKEIARLRAS"
     gene            complement(3686..>4347)
                     /locus_tag="VE170_00685"
     CDS             complement(3686..>4347)
                     /locus_tag="VE170_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYJ13974.1"
                     /translation="HRGTLFLDEVGDISLELQPKLLRVLQEQEFERLGSSRTLHVDVR
                     LVAATNADLTQKVTDNQFRADLYYRLNVFPVTIPPLRERREDIPLLVRYFAQKYARRM
                     KRPIDTIPVKSMTALTEYHWPGNVRELENFIERAVILSRGAELQIPLAELKQRAAGPS
                     IAASNGLATLENAEREHIVRALGETKWVIGGATGAAIRLGMKRTTLQSRMRKLGITRP
                     I"
BASE COUNT          933 a         1252 c         1176 g          986 t
ORIGIN      
        1 agcaaatttt cactaagcag catggcatac ccgctttgaa aaatcgcctg cgccggaacg
       61 tcggtaggat cgttttttat taatgggaat agcgtggtga gattgcgaca gctgacttcg
      121 atgtctcgat aaaagcgcgg ccgacggttc ttcgggcgcg tacttatgcc gcatgtgctc
      181 gaacagggcg ggttggtggc ctttaggata ctgatgccgc ttaaagcacg acgaacgata
      241 acttggccca atgcatttca agcagggcca tgccgcgatt aagaggggac gctataccta
      301 tctctatcgt tgtgcacctg ttgccgccgg aaagattcac tccagattct ttgtacagct
      361 tgtcttgcgc cgtaatcgac acggcggcgc agccgggcaa actcgtgtcg ccgctcagca
      421 actcggtgcc ggtcatcacc tgaacgggat ctggggcatg attttactct gttcgagggg
      481 aaaattattg ccactgctcg tgcgccagcg ctcggaagta gccgccgtca gtgcgcaaat
      541 ggtgcagacg cgtctgaaac caggtacccc ggggccagtg ccaattctgt gggggtagct
      601 gatcgccggc gtagccgccg aggtttacct tactttggcg aaagattagt tcccatggat
      661 tcatgtcggc tctccttttt gcggttatac ccggcgaagg cttaggtcag ctggccagcc
      721 tatcctagtg ttaggccgcg ctcggcagct cggttttatg atgggtctcc ttccatcgct
      781 gcagtccgcg gcgcaaatat tctggattta agccgaggga ttcacaaaca ttgtcaaaag
      841 aaaaagcccg atcgccgccc ggcgtaagga tccactcttc ggcttcgtcg aaaagccgcc
      901 gatctttccc acgctgggaa aacaaattat cccggtagca attgaccgca tcgtcgagga
      961 ccgccagcat gaggcgcttt tccggttcca gaaacgtggt tcgctgcatc gtgtcaaggt
     1021 actgtgccgg cagcagtgta tcgggttgaa aaagcgcatt gattctttcc tccccccggt
     1081 ggacggatat attgcccgcg tccgctgtat tggtgttcgt ccgttccatg atcctctcct
     1141 cctaattggc ctggtctacc tgaaggtcga tggcctgcgc gatgacttct ttgcgggata
     1201 cgccgaccat gcgctgtttt tcttcgccgc cgcgaaacag tatcagcgtc ggtatcgctt
     1261 gcaccccata gcgctgggca gcctgggggt tgtcatcgac gttaactttc atgaattgca
     1321 ccgcgccggc gtactgatcg gcgagtgcat cgacgatcgg cgtcagggcg cggcacggcg
     1381 cgcaccaggg cgcccaaaaa tccaccagca ccggtttatt cgtgttcagc accgcatcgg
     1441 taaaattccc atcgttggcg ttttgaacaa attcactcat ggtttttctc ctccgctaat
     1501 ctcctcgaca atcgctttgg tcatgcagcc ctcggccgcc agcacagctc cttgccggca
     1561 catctcggcc aacagttgtc ggcccgcgcc gtcgataaac gtcacctgct tcaacaccac
     1621 gtcgatctga cgatgctcgt tcgccacccg ctgccaacat tcgcgcagtt cctgcaccca
     1681 gggaccggcg agccgtccct cgagttccag gttggcgtgc tcgccttcag tatcgatggt
     1741 gattttaagc atgatcctgg acagcgcgct ttatataacc tcgccgtcta cgactgcgac
     1801 gaatacacct tcgagctctg ctctgccctc caccagcgcc cggtcgacaa tcaacctctc
     1861 gatgccgaaa cgatcgaacc agttaaccgc gacaagcgcg ccgccgccgt cgaacacttc
     1921 gagataatcc accggcctgg gcgtcgaccg atcgtcgtag atcgctatgg ctcccacggt
     1981 ctggccattg cccaacagcg gcgcaatgga gaccagagta aagggaactt gtgccagttc
     2041 tgcctccagc cttgcgtgtg gcggttgcgc cccgccgtca tcggcctgcg ccccggcggc
     2101 ggcgatgcaa ctcaacaaaa tgattccgcc gataactctt acggcggccc gcggatagtt
     2161 tttaggttcc aatgacataa tcgagatcct cttaagtctc agctcgccat atctcggcgc
     2221 ggcggttggc ggtttatcga tgcgagcgaa gaattttgcc cgcttctctg attcgcctcg
     2281 ccgaccactc gattgtcgtc gcaactagcg ctgtttcgat tccctggctt agtattctcc
     2341 ccaatagagt tgtcccgccc gccagcaaca ccgcgacaaa catactttcg agcttcatcg
     2401 gcactaccgc cttcctcttt cgctgcaatt gatactggca aggcccatgc cagcaacagc
     2461 cactgcccgc ctgaattgag taagtgatta taattacgag agataagtgc ctaatggatt
     2521 tcggcggtgg ggcttggcgg cgaaattaaa gtgacgaccg gtcggcacgc gccgaaatga
     2581 tggcttgatc aatcgtcgtt tcgtcgccaa ccattggctg acgatcatga gagataactt
     2641 ccattagcca gaaaatgatg tcgatgaacc ggatcgccag attgaaaatc agcgttagtt
     2701 tccgctcgct gcgatggcgg accgcaatct ggaggcgcgc ttaaggttgg atccgatcgc
     2761 cgaaattggc gcaggcttga aggaagttag actgagtatt gtagtcgatg gactaactga
     2821 agacggtgga gacgctttgg atgaatcaca atgcggcgag tgccctccgg ccgctcaaga
     2881 gccgttaagc gagctgcaat acccggtcgg catcgtgggt cattgccatg acaatcttcc
     2941 agctctcggc gtttttgcgc aacacatagg tctcgccaag ccgttcaagc tcctggccgt
     3001 ccgctttgta gcgaatccgt ttcacgctca ctacggcgat gtgggcgctc atctgattga
     3061 ctttcagctc tgacaattcg ctgcgcgtaa atccgcgcgc cttcagacct tccatgagct
     3121 gggcgataaa cgccgtcact tctccggtat ccttcatcat gcgcgtgccc tgcgcagaga
     3181 tgaacaaaca cggagcgtca caataaggaa ccactccgtc cggctttaga ctccgaaagg
     3241 actctacgta atcgcgaaat acctgagtga ccatctgttc atcgcctgcc atagcaaaac
     3301 tcctggcgaa gttcgcgcca agcgaagctg actccacctt tgcggtgtcg gtgatttcac
     3361 ttgaaagaac ggtcgcggac gccgcgcgcg tctgggtgct tgggatcgat ccaagcaagg
     3421 gctctgtcac atcgaacaaa cagcaaaaca tcaatgcgca attacttgca ctggcttacg
     3481 ttaaggagat tgcccgcctc cgcgcaagtt agactgggtc ctataacatc tagtttgaac
     3541 aagaattgtg ccaagaacgg caggcgctca ggccgaccaa gcaaatgctt cacgtcgaag
     3601 cacttgcacc ttcgttgggc gaaggcagca acagatgtga cgaaaaattt aagacgagtt
     3661 atcggcaggc gccaaaataa aggcgtcaga tcggacgagt gatgccgagt tttcgcatgc
     3721 gcgattggag cgtggtgcgc ttcatgccca accgaatggc ggcgccggtg gcaccgccga
     3781 ttacccattt agtttcaccg agagcgcgaa cgatatgttc gcgctcggca ttctcgaggg
     3841 tagctaaacc attcgaagca gctatcgaag ggccggctgc tcgttgtttc agttcggcaa
     3901 gcgggatttg cagctccgcc ccgcgcgaaa gaataaccgc gcgttcgata aaattctcca
     3961 actcgcggac gttgccgggc caatggtact cggtcagcgc ggtcatcgat tttaccggga
     4021 tagtatcgat gggccgcttc atgcgccggg cgtacttttg cgcaaagtaa cgcaccagca
     4081 ggggaatatc ctcgcgccgc tcgcgtagcg gcgggatcgt caccgggaaa acattcagac
     4141 gataataaag gtcagcgcga aactgattgt ccgtcacttt ttgcgtgagg tccgcatttg
     4201 tagcggccac caggcgaaca tccacatgaa gggtgcgcga gctgcccaac cgttcgaatt
     4261 cttgctcctg caaaactcgt aggagcttgg gctgcaattc gagagaaata tcgccgacct
     4321 cgtcgagaaa cagcgtgcct cgatggg
//
LOCUS       DASQKS010000030         5104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1696548, whole genome shotgun sequence.
ACCESSION   DASQKS010000030 DASQKS010000000
VERSION     DASQKS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696548"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VE170_00690"
     CDS             <1..555
                     /locus_tag="VE170_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HYJ13975.1"
                     /translation="NRQLPENSPEARELGDMIVAALERNPLFITATLPLRVFPPLFNR
                     YEDGESFGAHLDNSIRQIPGTPLRVRTDISATLFLSAPEDYDGGELVVDDVYGAHAVK
                     LGAGDMVVYPASSLHHVKPVTRGVRLASFFWVQSMVRDDGTRTLLFDLDMAINKVNQA
                     LPNHGAVVELTNCYHNLLRRFAEV"
     gene            557..1087
                     /locus_tag="VE170_00695"
     CDS             557..1087
                     /locus_tag="VE170_00695"
                     /inference="COORDINATES: protein motif:HMM:NF022893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HYJ13976.1"
                     /translation="MNRRSRTISAIALVLVAGCAAAAPVEESAARISVAFIDPEKFTD
                     ARRAELEPTSAGVVGELQKFIITTGARYMPENMKMNIRVTDIDLAGDFELFRGPTADQ
                     VRIVKGLYPPHIVLEFEIVDSAATVIKSGKRDLTDINYQLRSVYPREDHLRYEKDILR
                     DWLRAELGVLKTAKTN"
     gene            1104..2951
                     /locus_tag="VE170_00700"
     CDS             1104..2951
                     /locus_tag="VE170_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HYJ13977.1"
                     /translation="MKLLHKIIFWSHLLAGVIGGTVIFIMSATGVILMYERQLVEYAE
                     QDVRDVVPPGANAQRISLDELVAKARAQNPKAQPTGVVLRNEPTASVAVGFGRAGATY
                     VNPYTGVILGSESKLHDWFHDVVDWHRWLGTEDEGRVAARAITGACNLAFFWLALTGV
                     YLWWPRSWHWRGLKPSLLFNFHLRGKARDWNWHNVIGFWSSSVLIILTLTAAVMSYPW
                     ANDLLYTLTGSEPPPRAQAPGPAGQAQQRRGAGAETRERKPMASFDAFLARADQQVPG
                     WIMMSMRLPPRGDGPVTVLIQEPTAPHIFARSQLALNRSSADVIKWEPYAAASAGRKL
                     RVWVRGLHTGEALGFAGQTVAGLASLGGCFLVWTGFAMAWRRFRYRKRDAEDATTVEY
                     VTGPEASPAGVQTAPQTNEVSNDATRMEATRMEMEETNSSATNGHAHNGIAPNGLGNT
                     RPDWTGAADNGDSVLILYGTVTGNAELLANKLSATLSHAGVTARVRDIAHCQPSVLTQ
                     SNCVLVVISTYGDGEPPDDAATFWETVVQGNSLDLRGVKFSVLALGNTTYDHFCKCGR
                     DFDAALERHGGIRIHPRVDCDVDYEAPAKHWLDGVLAHLEKSERTANSA"
     gene            2971..3651
                     /locus_tag="VE170_00705"
     CDS             2971..3651
                     /locus_tag="VE170_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HYJ13978.1"
                     /translation="MAKLIATFTILATLTLISAAHAHFLWLEREGDGPARAYFGEWIA
                     DVREKTGGLLDRFKAPRVTVGANSEPLQVKRNDNNLEFAAKGAGDVRFVDSSVPARED
                     NDKGGTTKTIYYAKHGRAETAAKLDLELVPAEANGKTFVLIFSGSPLPKAELTIIGPS
                     KWEKPLTTDEQGRITLPMPWAGRYVLEVTHFDDKAGGSGGEKFNRTRHISSLSFVQQD
                     GIRWTEKP"
     gene            3672..3965
                     /locus_tag="VE170_00710"
     CDS             3672..3965
                     /locus_tag="VE170_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13979.1"
                     /translation="MMDTLVQKAWELYQHTALFPVETILTATVWLEQKYRKTPGEKIA
                     LAIALHYLILALRHDAGMGSATAEEYCARAARWQARACESNAVAPTAKGTEEN"
     gene            4193..4756
                     /locus_tag="VE170_00715"
     CDS             4193..4756
                     /locus_tag="VE170_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYJ13980.1"
                     /translation="MIQIGQKIDDFEFEVYQNQQIKKLKLSDYRAKWLVLLFYPADFT
                     FICPTELEEAAEYYGEFKKEGAEILSVSTDTVFVHKAWQNNSPSIKEITYPMAADPTA
                     QLCRYFGTYIDAAGLSLRGTFIVDPDGVLKAMDIHDNSIGRSAEEILRKLQAASFVRQ
                     HEGEVCPASWKPGKQTLKPGLDLVGKI"
     gene            4886..5071
                     /locus_tag="VE170_00720"
     CDS             4886..5071
                     /locus_tag="VE170_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13981.1"
                     /translation="MNGGNAEKKWEVTLGPEPLVHVHYATASPEVISDGFEGLARELR
                     DVADQLDAIARRINRVL"
BASE COUNT         1121 a         1545 c         1485 g          953 t
ORIGIN      
        1 aaccgccagc taccagagaa tagcccggag gcgcgggagc ttggcgatat gatcgtggca
       61 gccctggagc gcaacccgct gtttatcacg gcgacgttgc cgctccgggt cttcccgcct
      121 ctattcaacc gctacgaaga cggcgaatct ttcggcgcgc atctggacaa ctccatccgg
      181 cagattcccg gcacgccgct gcgcgtgcgt acggatattt ccgcgacgct gtttctcagc
      241 gcgccggagg actacgacgg cggcgagctg gtcgtcgacg acgtttacgg cgcccatgcg
      301 gtcaagcttg gcgccggtga catggtggtt tatccggcga gcagcctgca ccacgtcaag
      361 ccggtgacgc ggggagttcg tctcgcgtcg tttttctggg tgcagagcat ggtgcgcgac
      421 gacggcactc gaactctgct tttcgatctc gatatggcga tcaacaaagt caaccaagct
      481 ctgcctaatc acggcgccgt ggtcgagcta accaattgtt atcacaattt actgcgccgc
      541 ttcgcggagg tctgacatga ataggcgatc aagaacgatc tcggccatcg ccctcgtgct
      601 ggttgccggc tgcgccgcgg ccgcgccagt ggaggaatcc gccgcgcgga tctccgtcgc
      661 cttcatcgat ccggagaaat tcaccgacgc gcgccgcgcc gagctggagc cgacatcggc
      721 gggcgtcgtc ggcgaactac aaaagtttat catcacgacc ggcgcgcgat atatgcccga
      781 gaatatgaaa atgaatatcc gggtcaccga catcgacctc gccggcgact tcgaattgtt
      841 tcgcggtccg acagcggacc aggtgcgcat cgtcaaaggt ctctacccgc cccacatcgt
      901 gcttgagttc gagatcgtcg atagcgccgc gaccgtgatc aagtccggca aacgcgactt
      961 gaccgatatc aattaccaac tccgcagcgt ttacccgaga gaagaccatc tacgttacga
     1021 aaaggatatc ctacgcgact ggctgcgcgc cgagcttggg gttctgaaga ccgccaagac
     1081 caactagcac ccgaggagac ggcatgaagc tactgcacaa aatcattttt tggtctcacc
     1141 tgctcgccgg cgttattggc ggcacggtca tcttcatcat gtcggcaacc ggcgtcatcc
     1201 tcatgtacga gcgccaactg gtcgaatacg ccgaacagga cgtgcgcgac gttgtgccgc
     1261 cgggtgccaa tgcgcagcgc ataagcctgg atgaacttgt agccaaggcg cgcgcgcaaa
     1321 atcccaaagc ccagcccacg ggcgtggtcc tgcgcaacga accgaccgca tccgtcgccg
     1381 tcggcttcgg tcgtgcgggc gcaacctatg tgaatccata taccggagtg atcctcggct
     1441 cggaatcgaa actgcatgac tggtttcacg acgtcgtcga ttggcaccgc tggctcggca
     1501 cggaggacga aggccgtgtc gccgcgcgcg ccatcaccgg tgcttgcaac ctcgcgtttt
     1561 tctggctggc gcttaccgga gtctatctct ggtggccgcg cagttggcat tggcggggat
     1621 tgaagccgag cctgcttttc aatttccacc tccgcggcaa agcgcgcgac tggaactggc
     1681 acaacgtcat cggtttttgg tcgtcgtcgg tgctcatcat tctcaccctc actgcggcgg
     1741 tgatgtctta tccttgggcc aacgacctgc tctacacgtt gacgggcagc gagccgccgc
     1801 caagagcgca agcccccggc ccggcgggtc aagcgcagca gcggcgcggc gcgggcgcag
     1861 agacccgcga gcgcaagccg atggcaagct tcgatgcctt cctggcgcgc gccgatcagc
     1921 aagtgcccgg ttggatcatg atgtccatgc gcctgccgcc gcgcggcgat ggccccgtca
     1981 cggtcctgat tcaagaaccg acagcgccgc atatttttgc gcgctcgcaa ctggcgctca
     2041 atcgctccag cgccgacgtc ataaaatggg agccctacgc cgccgccagt gcgggacgaa
     2101 agctgcgagt ctgggtgcgc ggccttcaca ccggcgaggc tctcggtttc gccggccaaa
     2161 ccgtcgccgg cctggcgtcg ctcggcggct gtttcctggt ttggaccgga ttcgccatgg
     2221 cctggcggcg cttccgctat cggaaacgcg atgccgaaga tgcaaccacc gtggaatatg
     2281 taaccgggcc ggaagccagt cccgcaggcg tgcaaaccgc gccgcagacc aatgaagtat
     2341 caaatgacgc aaccagaatg gaggcaacga gaatggagat ggaagaaacg aactctagcg
     2401 caaccaacgg ccatgctcac aacggcattg ctcccaacgg ccttggaaat acccgccccg
     2461 actggaccgg cgcggccgac aacggcgatt cagtactcat tctctacggc accgtaaccg
     2521 gcaacgccga gttgctggcg aataagttgt cggcgacgct aagccatgcc ggcgtcacgg
     2581 ctcgcgtgcg agacatcgcc cactgccagc ccagcgtgtt gacgcagtcc aattgcgtat
     2641 tggtggtgat cagcacctat ggcgacggcg agccgcctga cgatgccgcg acgttctggg
     2701 aaacggtggt gcaaggcaat agcctcgatt tgcgtggcgt caagttttcc gtgctggcgc
     2761 tcggcaatac tacctacgat catttctgca agtgtgggcg cgacttcgac gccgctttgg
     2821 agcgccacgg cggcatacgc atccatccgc gcgtcgattg cgacgtcgat tacgaggcgc
     2881 cggccaagca ttggctcgac ggcgtgctgg cgcatctcga aaaatctgaa cgaacggcaa
     2941 actctgcgta gaaatcagga tgatacctcc atggccaagt tgatcgccac cttcactatc
     3001 cttgcgacgt taacgctaat ttccgcggct catgctcatt tcctctggct cgaacgcgaa
     3061 ggcgacggcc cggcgcgcgc gtacttcggc gaatggatcg ccgacgtccg cgaaaaaacc
     3121 ggcgggctgc tcgaccgatt caaagcgccg cgcgtgactg tcggcgcgaa tagcgaaccg
     3181 cttcaggtca aacgcaatga caataacctc gagttcgcag ccaagggagc gggcgacgtg
     3241 cgcttcgtgg acagcagcgt gccggcccgc gaggacaacg ataaaggcgg aacaaccaag
     3301 acaatctatt acgccaagca tgggcgtgcg gaaaccgcgg cgaagctcga tttggagttg
     3361 gtgcctgccg aagccaacgg taaaaccttt gtgctaattt tttccggttc accgcttcct
     3421 aaagccgaac tcacgatcat cggcccatcc aagtgggaaa aacctctaac caccgacgaa
     3481 caaggccgta tcactctgcc gatgccgtgg gcgggtcgtt acgtgttgga agtgacccac
     3541 ttcgatgaca aggccggcgg cagcggtggc gaaaaattca accggacgcg ccacatatcg
     3601 tcgctttcct tcgtgcagca ggacggcatt cgttggacgg aaaaaccgta atctcctaga
     3661 tcggcaggtg aatgatggac accctggtgc aaaaagcatg ggagctttat caacatacgg
     3721 cgctatttcc ggtggaaacg atcctcacgg ccactgtctg gctcgaacaa aagtaccgta
     3781 aaacgccggg cgaaaaaatc gccctggcaa tcgcgctgca ctatctgatt ctcgcgctgc
     3841 gccacgacgc cggcatgggc tccgcaaccg cggaggaata ctgcgctcgg gcagcgcgct
     3901 ggcaggcgag agcgtgtgaa tcgaacgccg tcgcgccgac ggcaaaagga acggaggaga
     3961 actgaccaag attcgacccg atcgcggcgc caattcgagc gccaaatata gagtttcacc
     4021 caaaaactaa atcacgccag ccatccggcc ttccggccca gacagccagc cacctgcaga
     4081 gtagtaatcg agatcgccga gatctcatga aacaccgatc gtcggcgttt gcggagctat
     4141 gaaagatcaa cggtatttat cgaccgctat cacaacagaa ggagccatca ccatgattca
     4201 gatcggccaa aagatcgacg acttcgagtt cgaagtttat caaaaccagc agatcaaaaa
     4261 acttaaattg tccgactacc gagccaagtg gctcgtgctg ctcttctacc ctgctgattt
     4321 cacgtttatt tgcccgacgg aattggaaga agccgcggaa tactacggcg agttcaagaa
     4381 ggaaggcgcc gagatattga gcgtcagcac ggataccgtt ttcgtccaca aggcgtggca
     4441 gaacaactcg ccgtcgatta aggaaatcac ctacccgatg gccgctgatc cgaccgcgca
     4501 gctttgccgg tacttcggca cctacatcga tgcggccggt ctgtccttgc gcggtacttt
     4561 catcgtcgat cccgacggcg tgctcaaggc catggatatc cacgacaata gcatcgggcg
     4621 cagcgccgaa gaaatattgc gcaagctgca ggccgcgagt ttcgtgcgcc agcatgaggg
     4681 agaagtctgt cccgcaagct ggaagccggg taagcaaacc ctgaagccag gattggatct
     4741 agtcggcaag atttagtgat ctcggttaga gaacaaccca aggtcgtgtc gggatgaatc
     4801 gacaagaacg gcaatcggtc aggagctgac tcatacgctc ctgtccggaa tcaccgcgcg
     4861 gtaaagcggt acaaaggagc aaaccatgaa cggtggtaac gcggaaaaaa aatgggaggt
     4921 cactttgggt ccggagcccc tggtccatgt gcattacgcc acggcatcac ccgaagtgat
     4981 cagcgacggg tttgaaggat tggcacggga gctgcgcgac gtcgccgatc agctcgacgc
     5041 gatcgcgcgg cgcatcaatc gcgtgctcta atttttttac gaaatccccg ccctttttgt
     5101 ctgt
//
LOCUS       DASQKS010000031         9218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5896540, whole genome shotgun sequence.
ACCESSION   DASQKS010000031 DASQKS010000000
VERSION     DASQKS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5896540"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..561
                     /locus_tag="VE170_00725"
     CDS             286..561
                     /locus_tag="VE170_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13982.1"
                     /translation="MNPHIIDAAGWLPAIIFPIATLIQLIALLRTNNLQGISPTTWTM
                     FGIANISVYIYTEKYLAVQTIVGFLGTALLDFMIAAMAIKGRAAEKT"
     gene            complement(635..2398)
                     /locus_tag="VE170_00730"
     CDS             complement(635..2398)
                     /locus_tag="VE170_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ13983.1"
                     /translation="MATTRSLPLGRSVSLRRLVSPLVDFKSAGSILVLIVLTVLVIVP
                     LLIMLLASVRPAGVMPFASSVFTFQGYAQAYGGGDLLPVIRNTLIYAGLSVVFALPIA
                     FGFAFLTERTDMPLRNAMYVLMFIPMSTPVFATALGWILLLGPRGGTINVYLRALIGS
                     DAAEGPFNIFSLPGLIFVHVLGMVPTMWLFLTAVLRRMDPVLEEAASAAGAGRWRTLR
                     TVTAPLMRPGVAAVAIYFFLSGLESLELPLALGPTAGIEVLATKIFFSLAPTADLGVN
                     YGIPAAFGLLGLLMGVAGTILYHHLVRQASDFAVVTGKGYRPRIIELGGWKYLALGVI
                     GLFVIVKVVLPFAVLLYASFLRFYVPPIAEYLPDIKWTLIHYERLFDYRFFGRFFVNT
                     LIVAVLAATLTMLLVSFIGWLVVRHPSPLSKAINLVAFMPLAIPGVISTLALFLMFVG
                     TPLHGTLALIMLAFLARYLAFGTRLMHSAVLQIHKELEEVSHTSGAGSFQTFSRITLR
                     LLFPAFMNGWLWVFVHAAKDFSVALMLASAGSVVVANVIYESFVGGHFNSSAAMLVVL
                     IAFNLLFVIAGRKWIGATLAQ"
     gene            complement(2455..3618)
                     /locus_tag="VE170_00735"
     CDS             complement(2455..3618)
                     /locus_tag="VE170_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13984.1"
                     /translation="MNASKFLTVWLAWLFAFIAPMAFAAQGAPGELANLKELIAKAKA
                     SSRTVTLALEATEPDVVRGKEKAFEKKFGFPVKLESQPGHHRDMPVKVIESAKSGRGV
                     IDSWDGGTPLILGMFRAGNTRRPPWEAIYEGWPLAKKLRSVVPQIGGGPDGAVLSDHC
                     MHAQMGNWSLVYNTRRVKAAELKGIKLEDLTGERWRNRVLWDAQALGLFALPFAPGWD
                     VERMKTFAHNLGANGTKMVAGGSMNLIQSLIQGEGDVSMVSMNWVLPQKKLGAPLDIA
                     FAEFVLGNSTVTCLIKPPVNDAHMTALYWAWDAFDGTYTEAQISGAGAFRLFAEEEKF
                     LPLARLARQNGVTKMEQVVGPKTEEDAEKASEYRKIAIQALKDGISSKKKIAK"
     gene            complement(3665..4906)
                     /locus_tag="VE170_00740"
     CDS             complement(3665..4906)
                     /locus_tag="VE170_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ13985.1"
                     /translation="MARHYKCISADSHLDIRPERWTPRVPKRWRERAPRTVVLANGNE
                     GILVENRPPRSPGATTITGLPYEKHGLQKYWFEGPGTGAPEQRLWEQDQDGVDAEVLY
                     SHGSYAEFWRGIADDDAYKAMIRAYNEWLAEEYCAYNPHRLLGMGVLPDTGVDDAIAE
                     MTYCATAGLKGVNLHRFPSGKGYVTAADDEFWSAALDLGICVTSHTNGGTTRLGAKND
                     GVFQYPRALVKSGGGERDPVSLLFRFAGEQPMAPLQMAMAGVFDRFPRLKIYWAESQC
                     GWLPYSFDQIDDNYERNRYWGERDFAFPGPQRRPSEYLKEHCLWGFMRDAWGIKCRHE
                     IGVDILLWGSDFAHATGDWPNSQRLLDETFVGVAPLERQMMVLDNAVRYFHLDASADL
                     DLYGQAMQGMSIEQFDARLSG"
     gene            complement(5023..6165)
                     /locus_tag="VE170_00745"
     CDS             complement(5023..6165)
                     /locus_tag="VE170_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ13986.1"
                     /translation="MARQYKIISGDSHLDLPPDRWTHRVPAKWKDRAPRRVRMADGEE
                     AIAMEGRPIAPIGLTRSVGVAHDQLHLQVPTFDNSAGTGQPEQRLREQDQDGIDAEIL
                     FSRINIIRGIKEDEGYVALNHAYNEYLAEEYARAAPQRLFPMGIIPTTGIGDAVKELD
                     YCAKAGLKGVQLDMFPNGKQFPTPADDRFWAAALDQGIAVTSHTRAGTTRFAAKGPFF
                     QYPKAGYKGDPIEQIFRFCGDAAFAVLQMAFAGVYERFPTLQLYWAETQIGWLPYALW
                     QVDDHYQRYRHLAKGMFGLDFLKRKPSEYICQHSLWGFLYDKIGVGTRQAIGSDKVIW
                     GSDFAHAASDWPNSKKSIASSFAGVPEDERYAMLAGNVIRFFRLDG"
     gene            complement(6226..8415)
                     /locus_tag="VE170_00750"
     CDS             complement(6226..8415)
                     /locus_tag="VE170_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ13987.1"
                     /translation="MSEIISPAVELAPLREMLGFYCHALTERRVELQDLKQLIDKNIG
                     WSKSDVATSDGAAIFLPAVVERFDARADNYDYLKVMLTQQAGHLEFGSFEFKFERPST
                     QFTDLRPQFKAPAEYHDHDHGHEGHHEQAAVTELTRFFRLFPNKRLALDIFSLVESAR
                     IEARIMLEYRGIAVAYSAMRGRTLALRQESIYLPAREALLEFMVRLSLGRTDGIKVPK
                     KQAAVARDIRALMQLVAGKTATVEDSAEATLRIYARLARVKNDYLAEDQFVFLELADR
                     SSSRSKRSSRSNRWEDREEVAVLPELNGREREYLAPQGVDYRGEFRPELAQLLTQAQS
                     NSREQRKSLTPEQLADLLRSQRAPKPRDGEDDGDDQDPQTSQMVQNLLQELDQRDPRM
                     QSVTRRPSPNNDDDSGPLSATQPNTFIYDEWDAAARQYRQRWCKVYEKNLASGDLTFY
                     RETLLSHAGLSQQIRREFEQMVPELYHKEKRLPEGADHDLDLAIEALTDFRIGISPSE
                     KIFWRHHKIERDIAVAFLLDMSGSTGEAIKTVGDKPLPGSGAERSQRRIIDVEKEAIV
                     LMMDSLEAIGDRYGIYGFSGHGRDNVEFYVIKDLDEAFSMDIAKRFGRIGPLHATRMG
                     PAIRHTMAKLRKQQSHSKFLFLISDGRPQDRGYSQEGAEKAHAVQDTRMAMIEARREG
                     IHPFCLTVDKDGNDYLRTMMDDFSYEVLADVSLLPRRLPQLYKKLTT"
     gene            complement(8605..>9218)
                     /locus_tag="VE170_00755"
     CDS             complement(8605..>9218)
                     /locus_tag="VE170_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HYJ13988.1"
                     /translation="CHEDLTASDLVGRYLLDAAGTRWIDGPLTRAVRVGGICYLDEIV
                     EARKDTTVIIHPLTDHRRLLTIDKLGEVVEAADSFLLVVSYNPGYQNVLKDLKHSTRQ
                     RFVALEFQFPSTEREAKIIQHESGVDEPSAVQLAKLGHKVRNLREHGLKEGASTRVLI
                     YAGRLIKEGIAPRRACQVAVTWGITDDPQVQRSLEEVVNTIFP"
BASE COUNT         1953 a         2823 c         2572 g         1870 t
ORIGIN      
        1 cctgttttca ttcaccgaca ggccgctgct ggagatgacg ggattggata gggaggagaa
       61 tggctaggca gcctaccgaa ctctgattct tcaaagtgcg gccgcttggc acacgcgaac
      121 ttcatgtatt gaaatcggcg ctagtccagc cgtgtcggct ttcgattctc cttcatcggc
      181 cacgcgacct tgaaactgtt ttcttgcctc gacgggcatg acattgacgc atccgctact
      241 tcgctctaac cgtactaagg cctgccaaag atttgaaatg gcaaaatgaa tccgcacatt
      301 atcgacgccg ccggctggct cccagcgatc atcttcccga tcgcaacatt gatccagctc
      361 atcgccctgc tgcgaactaa caatcttcaa gggataagtc cgacgacctg gacaatgttc
      421 ggcatcgcga acatctcggt ctacatctac accgagaaat atctcgcggt tcaaacgatt
      481 gtcggctttt tgggcacggc gctgttagat ttcatgatcg ccgcgatggc aattaaagga
      541 cgcgctgcag agaagacatg attcgctgag tcagggtgcg aacgatttga actttttgaa
      601 cggcttgaac gttttgaacg ctcccatttt cgcgctattg cgccagggtc gcgccgatcc
      661 acttccgccc ggcgatgacg aatagcaaat tgaatgcgat caaaaccacc agcatggcag
      721 cggaggaatt gaagtgtccg ccgacaaagg actcgtagat gacattggca acgacgacgc
      781 tgccggccga agccaacatc aaggccaccg aaaagtcttt ggccgcgtgc acgaataccc
      841 atagccagcc gttcatgaag gcgggaaata gcaagcgcag cgtgatccgc gagaaggtct
      901 gaaaacttcc agcgccgctg gtgtgggata cttcttccag ctctttatga atctgtaaaa
      961 ccgctgagtg catcagacgg gtgccgaaag ccaggtagcg cgccaggaac gccagcatga
     1021 tcagcgccag ggtgccatgc aacggcgtgc cgacgaacat caggaacaac gccagggtcg
     1081 agatcacccc cggaatcgct agcggcatga aggccacgag attgatcgcc ttgctcaacg
     1141 gcgacggatg gcgcaccacc agccagccga tgaaactcac cagcagcatg gtcaacgtcg
     1201 ccgccagcac ggcgacgatc aatgtgttga cgaaaaacct gccaaaaaaa cgatagtcga
     1261 acagccgctc gtaatgaatc aaggtccact tgatgtcggg caaatattcg gcgatgggcg
     1321 gcacatagaa gcgcagaaag ctggcgtaga gcagcaccgc gaagggcaag acgactttga
     1381 caataacgaa cagaccgatg acccccagcg ccaaatactt ccaaccgcca agctcgatga
     1441 tccgcggccg ataccccttg ccggttacca ccgcgaaatc cgatgcttga cggaccagat
     1501 ggtgataaag aattgtcccg gccacgccca tcaatagacc gaggagtccg aatgctgccg
     1561 gaatgccgta gttgacgccc aagtctgcag tcggcgccag actgaaaaat attttcgttg
     1621 ccaacacttc gatgccagcg gtcggtccta aagcgagcgg caactccaga ctctcgagac
     1681 cgctcaaaaa aaagtagatc gccaccgccg cgacgccggg acgcatgagc ggcgcggtga
     1741 cggtacgtaa agtacgccag cgcccggcac cggcggccga cgccgcctct tcgagcaccg
     1801 ggtccatgcg ccgcaaaaca gcggtcaaaa acagccacat ggtcggcacc atgccgagga
     1861 cgtgaacaaa gatcagcccc ggcaggctga aaatattgaa cggcccctcg gcagcgtcgc
     1921 tgccgatcaa cgcccgcaga taaacgttga tggtgccacc gcgcgggccc aacaataaaa
     1981 tccaacccaa ggcggtggcg aacaccggcg tgctcatggg aatgaacatg agcacgtaca
     2041 tggcgttgcg caacggcatg tcggtgcgct cggtgagaaa agcgaaaccg aacgcgatcg
     2101 gcagcgcgaa tacgacgctc agcccggcgt agatcaaagt gttgcggatg accggcagca
     2161 gatcgccgcc gccgtatgcc tgcgcgtaac cttgaaaagt aaacacgctc gacgcaaacg
     2221 gcatcacccc agcgggacga acgctggcga gcaacatgat taacagcgga acgatgacga
     2281 gaacggtgag gacgatgagg accaagatcg aaccggcgga tttgaaatca accaacggcg
     2341 agaccaaccg acgcaaactg acggagcgac caaggggcaa agatcgcgtc gttgccatcg
     2401 tggatccgaa aaaaaactgc ttgaactact tgaacgattg aacggcttga acgtctattt
     2461 tgcaattttc ttcttactgc taatgccatc tttcaacgcc tggatggcga tcttgcgata
     2521 ttcgcttgcc ttttccgcgt cttcttccgt cttgggccca accacctgct ccatcttggt
     2581 gacgccgttc tggcgcgcca gacgcgccag tgggagaaac ttctcttcct cggcaaacaa
     2641 ccgaaacgca ccggcgccgg agatctgcgc ttccgtgtag gtgccgtcga acgcgtccca
     2701 agcccagtac agcgccgtca tgtgagcatc attcaccggc ggcttgatca aacaagtgac
     2761 cgtcgaattg cccaggacga attcggcaaa cgcgatgtcc agcggcgcgc caagcttttt
     2821 ctgcggcagc acccagttca tcgacaccat gctgacatca ccctcgccct ggatcagcga
     2881 ctggatcaaa ttcatgctgc cgccggcgac catcttggtg ccgttggcgc ccaagttgtg
     2941 cgcgaatgtt ttcatgcgct cgacatccca ccccggcgcg aagggcaaag cgaacaaccc
     3001 caacgcctgg gcgtcccata gcacgcgatt gcgccaacgt tcgccggtca gatcttccaa
     3061 cttaatgccc ttgagctctg ccgctttgac ccgccgtgta ttgtaaacca gtgaccagtt
     3121 tcccatttgc gcgtgcatgc aatgatcgct caacaccgcg ccgtccggtc cgccgccgat
     3181 ctgcggcacc acagaacgca attttttcgc cagcggccat ccctcgtaaa tcgcttccca
     3241 gggcggccgg cgcgtgttgc cggcgcgaaa catgccaagg atcagcggcg tcccaccgtc
     3301 ccacgagtcg atcacaccgc gccccgattt cgccgactcg atgactttca ccggcatgtc
     3361 gcgatgatgg ccgggctgcg attcgagttt taccggaaag ccgaatttct tctcgaaggc
     3421 tttctccttg ccgcgaacca cgtccggctc ggtcgcctcg agcgccagcg tcaccgtcct
     3481 gctcgacgcc ttggccttgg caatcagctc tttcaaattc gccagctcgc caggcgcgcc
     3541 ctgggcggca aatgccattg gcgctataaa tgcgaatagc cacgcgagcc acaccgtgag
     3601 aaatttgctg gcattcattg atagtatcct ccgcttgacc cgaaatccga gcttcttcga
     3661 ccaattatcc cgacagccgc gcatcgaact gttcgatgct cattccttgc atcgcttggc
     3721 catagaggtc gagatcggcg ctggcgtcga ggtgaaaata tctaaccgcg ttgtcgagca
     3781 ccatcatttg tcgttcgagc ggggccaccc cgacgaaggt ttcatcgagc aaccgttgcg
     3841 agttgggcca gtcgccggtg gcgtgggcga agtcgctgcc ccaaagcaaa atatcgacgc
     3901 cgatctcgtg tcggcatttg atgccccagg catcgcgcat aaaaccccac aggcagtgct
     3961 ccttcaaata ctcgctcggc cgccgctgcg gcccggggaa agcgaaatcg cgctcacccc
     4021 aatagcggtt gcgctcgtag ttgtcgtcga tctgatcgaa gctgtagggc agccagccgc
     4081 actggctctc ggcccagtag attttcaggc gaggaaagcg gtcgaagacg cccgccatcg
     4141 ccatctgcaa cggcgccatg ggctgctcgc cggcgaaacg aaacaacagc gacaccggat
     4201 cgcgctcgcc gccgccggac ttgaccaatg cgcgcgggta ctgaaagaca ccgtcgtttt
     4261 tcgccccgag ccgggtcgtg ccgccgttgg tatgggaagt cacgcagatg ccgaggtcga
     4321 gggcagcgga ccagaactcg tcgtcggcgg ctgtgacgta acctttgccg cttgggaagc
     4381 ggtggagatt gacgcccttc aagcccgccg tggcgcagta agtcatctcg gcgatggcgt
     4441 cgtcgacgcc ggtgtcgggg agcacgccca tgcccagcaa gcgatgggga ttgtaggcgc
     4501 agtattcttc ggcaagccat tcgttatagg cgcggatcat cgccttgtag gcgtcatcgt
     4561 cggcaatgcc acgccaaaac tccgcataac tgccgtgcga gtagagaacc tccgcatcga
     4621 caccgtcttg atcctgctcc cacaaccgtt gctctggtgc gcccgtaccg ggtccctcga
     4681 accaatattt ttgcaggccg tgtttttcgt agggcagtcc ggtgatggtg gtcgctcccg
     4741 gactgcgcgg cggccggttc tcgacgagaa tcccctcatt gccgttagca agtaccaccg
     4801 tgcgtggcgc ccgttcgcgc cagcgcttcg gcacccgcgg tgtccagcgc tcggggcgaa
     4861 tatccaaatg ggaatcagcc gaaatgcatt tgtaatgacg cgccatggca ttctcctcgg
     4921 ggatactcaa gcatggaagt tttaaacccc ttcctacagg tcgggttttt tctttgcgac
     4981 tctgcggcca accattggca actctctgtc gttaagattt cgtcaaccgt ccaagcgaaa
     5041 aaatcttatc acgttgccag ccagcatcgc atagcgttcg tcctccggca caccggcaaa
     5101 gctggaagcg atgctcttct tcgagttggg ccaatcgctc gccgcgtggg cgaagtcgct
     5161 gccccagatc actttgtcgc taccgatcgc ctggcgcgtg ccgacgccga tcttgtcgta
     5221 aagaaaaccc cagagcgagt gctggcaaat gtattcgctc ggtttacgtt tgaggaagtc
     5281 gagaccgaac atcccttttg ccaagtggcg gtagcgctgg tagtgatcgt cgacctgcca
     5341 gagcgcgtag ggcagccagc cgatttgagt ctccgcccag tacagctgta acgttgggaa
     5401 acgttcgtaa acgccggcaa acgccatctg taaaactgcg aacgccgcgt cgccgcagaa
     5461 gcgaaaaatc tgttcgatcg gatcgccctt gtagccggcc ttgggatact gaaaaaacgg
     5521 ccccttggca gcgaagcgcg tcgtgccggc ccgggtatgc gatgtgaccg cgatgccttg
     5581 atccagcgcc gccgcccaga aacgatcgtc ggcaggcgtc ggaaactgct tgccgttggg
     5641 aaacatatcg agctgcacgc ccttgagtcc cgccttggca cagtagtcca actctttcac
     5701 cgcgtcgccg ataccggtgg tcggaatgat tcccatggga aacaaccgct gcggcgccgc
     5761 cctggcatac tcctccgcta agtattcatt gtaagcgtga ttgagcgcga catacccctc
     5821 gtcttctttg atgccacgta tgatattgat acgcgaaaac aggatctcgg cgtcgatgcc
     5881 gtcttgatcc tgctcgcgta agcgctgctc cggctggccc gttcccgcgc tgttgtcgaa
     5941 ggtgggaact tgtaaatgca actgatcgtg tgccacgccg acgctgcgtg tgagcccgat
     6001 tggcgcgatc ggccgccctt ccatcgcgat cgcttcttcg ccatcggcca tccgcacccg
     6061 ccgcggcgcc cgatccttcc actttgccgg cacccgatgg gtccagcgat ccggcggcaa
     6121 atccaaatgc gaatcgccgg aaataatctt atactgcctc gccattactt tatcccctca
     6181 actctggaac ggctggaaca tttggaacga ttagaaccac gacctttagg tcgtgagctt
     6241 cttatacaac tgcggcaatc gccgcggcaa caaactcacg tcggccaata cctcgtaact
     6301 gaaatcatcc atcatggtgc gcaaataatc gttgccgtcc ttatcaacgg tcagacaaaa
     6361 tggatggatg ccctcacgcc gcgcctcgat catcgccatg cgcgtatcct gcaccgcgtg
     6421 cgccttctcg gcgccctctt gcgaatagcc gcgatcctgg ggccggccgt cgctgatcaa
     6481 gaacagaaat ttcgagtggc tctgctgttt gcgcagcttc gccatggtat ggcggattgc
     6541 cggacccatg cgcgtcgcgt ggagcggacc gatgcggccg aagcgtttgg cgatgtccat
     6601 ggaaaacgcc tcgtccaaat ccttgatgac gtagaactcg acgttgtcgc ggccgtggcc
     6661 ggagaacccg taaattccgt agcggtcgcc gatggcttcg agggaatcca tcatcaaaac
     6721 aatcgcctct ttctcgacgt cgatgatgcg ccgttgtgaa cgctccgcgc cgctgccggg
     6781 caacggtttg tcgccgacgg tcttgatcgc ttcacccgtc gagccgctca tgtcgagcaa
     6841 gaatgccacc gcgatatcgc gctcgatctt gtggtggcgc caaaagattt tttccgacgg
     6901 actgataccg atacggaagt cggtcaaggc ctcgatggcc aggtcgagat cgtgatccgc
     6961 gccttccggc aagcgtttct ccttgtgata gagttccggc accatctgct cgaactcgcg
     7021 gcgaatctgc tgtgacaaac cggcgtggct caatagggtc tcgcggtaga aggtcagatc
     7081 gccgctggcc aggtttttct catagacctt gcaccagcgt tgccggtatt gacgcgccgc
     7141 ggcatcccac tcgtcgtaga taaaagtatt cggctgagtc gccgacaacg gcccggagtc
     7201 atcgtcgtta ttgggcgatg gtcggcgcgt cacgctctgc attcgcggat cgcgctgatc
     7261 gagttcctgc aaaaggttct gcaccatctg gctggtctgg ggatcttggt cgtcgccatc
     7321 atcttcgccg tcgcgcggtt tgggcgcgcg ctggctgcgc agcaagtccg ccagctgttc
     7381 cggcgtcagc gatttacgct gctcgcgcga gttggactga gcttgggtca gcaactgcgc
     7441 caactccggg cgaaactcgc cgcggtaatc gaccccctgc ggcgccaggt actcgcgctc
     7501 ccgcccattc aattccggca gcaccgcgac ttcttctcga tcctcccaac ggtttgaacg
     7561 actcgaacgt tttgaacggc tagagctcct atctgccagc tccaaaaaga cgaactgatc
     7621 ttcagccaaa tagtcgtttt taacccgggc caagcgcgca tagatccgta atgtcgcttc
     7681 cgcagagtct tcgaccgtgg cggttttgcc ggcaacgagc tgcatcagcg cgcgaatatc
     7741 gcgggcgacg gccgcctgct tcttggggac tttgatgcca tcggtgcggc cgaggctgag
     7801 ccgaaccatg aattccagca gcgcttcgcg ggccggcaga tagatcgatt cctggcgcag
     7861 cgccagcgtc cgcccgcgca tggcgctgta agcgaccgcg atgccgcggt attccagcat
     7921 aatgcgcgct tcgatccgcg cgctctcgac gagggagaaa atgtccagcg ccagccgctt
     7981 gttcggaaac agccgaaaaa atcgcgtcag ctcggtgacc gcggcctgct cgtgatgccc
     8041 ttcatggcca tgatcgtggt cgtgatattc cgccggcgcc ttgaattgcg gccgcagatc
     8101 ggtgaattgc gtcgacggtc gctcgaactt gaactcgaaa ctgccgaact cgagatggcc
     8161 ggcctgctgg gtgagcatga ctttgagata atcataattg tccgcccgcg cgtcgaagcg
     8221 ctcgacgaca gccggcagaa aaatcgccgc cccatcgctg gtggcgacat cgctcttcga
     8281 ccaaccgatg tttttgtcga tgagttgctt gagatcctgc agctcgacgc gccgctcggt
     8341 caacgcgtgg caataaaacc ccaacatctc acgcagcggc gccagctcaa cggcaggtga
     8401 aatgatctca gacatgggaa gtttggccgc agagaacgca aaggtcgcaa attaaaaatt
     8461 gccaacggct ttcgcgctaa atcttttcct cgcgtccttc atattgaatg ttaggctccg
     8521 tagaccctta actgcggttt gccgcatcaa gatccttatt ttgcgagctt tgcgttcttt
     8581 gcggccattc tttctttggt attctcacgg gaatattgta ttgacgacct cttccaaact
     8641 ccgctgcact tgcgggtcgt cggtgatgcc ccaagtgacc gccacctggc aggcgcggcg
     8701 cggcgcgatg ccttccttga tcaagcggcc ggcgtaaatc aacacgcgcg tgctcgcgcc
     8761 ctctttcaag ccatgctcgc gcagattgcg caccttgtgg ccgagcttgg cgagttgcac
     8821 ggctgacggc tcgtcgacgc cagattcatg ctgaataatt ttcgcctcgc gctcggtgct
     8881 cggaaactga aactcgagag cgacgaaacg ctggcgcgtg ctgtgcttta aatctttcag
     8941 cacgttctga tagccggggt tgtacgacac caccagcaaa aaactatccg ctgcctcgac
     9001 cacttcgccg agcttgtcga tggttaggag ccggcggtga tcggtcagcg ggtgaatgat
     9061 cacggtggta tctttgcgag cctcgacgat ttcgtcgagg tagcagatgc cgccgacgcg
     9121 aactgcccgc gtcagcggcc cgtcgatcca gcgcgtgccg gcggcgtcga gcaaataacg
     9181 cccgaccaga tcgctggcgg tcaagtcttc gtggcagg
//
LOCUS       DASQKS010000032         6528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3797148, whole genome shotgun sequence.
ACCESSION   DASQKS010000032 DASQKS010000000
VERSION     DASQKS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3797148"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VE170_00760"
     CDS             complement(<1..547)
                     /locus_tag="VE170_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HYJ13989.1"
                     /translation="MAKKSAAEAFRPPQPIPEFLRSLSYDDYRDIRFDTKRSLWRDSG
                     NFQIQYIHPGLFYGHAVKMNTVEGGVVRPTRFSPEQFTYGKNKFTDKIPADLGFAGFR
                     VTYPFHAKNEFNHVVVFAGASYFRAVAKGQVFGLSARGLAIDTGLPTGEEFPYFKEFW
                     LERPARDARQTTLYALLDSVSV"
     gene            complement(1808..2902)
                     /locus_tag="VE170_00765"
     CDS             complement(1808..2902)
                     /locus_tag="VE170_00765"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HYJ13990.1"
                     /translation="MNEFTHLRQQLGGILTALAVGIPAGFLFAWLHTPIPWMLGPMIA
                     VASLNLMGVTMHSPPYARQLGQVILGSAVSLYFTPPVVAALAGNLPAVLAATVAVFIV
                     GIFGALTLSKASGVDWKSTFFASIPGGAMAMAVLADRYGAQMSPVAVAHSLRVSVVVI
                     VVPFVLTYSGIPLEAAAYKPDLPLLPTILVVWLLGSGVLGEISERFHFHNGCLLVPIF
                     FGAALTMGGVELSAVPHSLTDFAQLMFGLVLGARYERAFFARHRLFIPFALLNSLFIL
                     IASAIAGVALALAFDLPIATMIIATSPGGLAEMTITAQALHISVPLVVAFHLFRVVVV
                     NMGTQHIFVAVSHLVGRAPTDGDPAPSRDR"
     gene            3081..4163
                     /locus_tag="VE170_00770"
     CDS             3081..4163
                     /locus_tag="VE170_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ13991.1"
                     /translation="MDYKSAVIDADAHVVESERVWDYLEDSEKKFRPTLMPSPENPKR
                     QFWVLDGENLGPKFPSPDEHESEAHLKRFGREVGTPVAARELSNVQQRLKHMDELGID
                     VQVLFNSLWITPLTSRADAEIALSWAWNRWLADVWRLGEDRLRWTCVVPALSPTEAVA
                     QIRFAKQNGAVGVCMRPFERDRMMTDRFYYPIYQEADRLDIPITVHLANGSPELFKLM
                     TNEAGGGFSTFRVPTVTSCYALIMSEIPQVFPKLRWGFIEVSSQWVPWVIHEAMRRSL
                     GSARPISRDCLREYNIYVSTQSDDDFPYILSYAGEGNLVIGTDYGHGDTSSELNAISR
                     FKAMTELSPEVKKKILFDNPKALYGI"
     gene            4187..4606
                     /locus_tag="VE170_00775"
     CDS             4187..4606
                     /locus_tag="VE170_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HYJ13992.1"
                     /translation="MATLISVEKEKQLAERMLALGVREADIDEQFVRSSGAGGQNVNK
                     VASCVVLHHRPTGLRVKCQQERSQALNRFLARRLLLDKIDTRQRGAASAEMQRIEKIR
                     RQKRKRSRRAKMRMLDDKHHQSEKKGRRSTVRPGRDD"
     gene            4852..4980
                     /locus_tag="VE170_00780"
     CDS             4852..4980
                     /locus_tag="VE170_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ13993.1"
                     /translation="MMNDLILLVLVVVLVPLAAIAILRMLGKTKRTKRPIRDFRVR"
     gene            complement(5701..6447)
                     /locus_tag="VE170_00785"
     CDS             complement(5701..6447)
                     /locus_tag="VE170_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYJ13994.1"
                     /translation="MRKLLVCQHVPHEILGTLNPMLKHAGFRIRYVNFGRHPDAQPNL
                     DGYAGLIVLGGPMSAYDDQRLPHLAVEMKLIESAMKRNLPVLGICLGGQLVARTLGAR
                     VYANPIKEIGWYDVAPSAHADDDPLLNDFKERETLFQWHGDTFDIPHGGIHLASSRHC
                     ENQAFRFGANVYAFQFHLEVDEPMILRWLKVPENRREIASLHGDIDPDRIHTETVRHI
                     GRLHELSDRVFGKFIENFGLEKSRRRLLSR"
BASE COUNT         1510 a         1742 c         1848 g         1428 t
ORIGIN      
        1 taacgctcac actatcgagc aacgcgtaca gggtcgtttg gcgcgcgtcg cgcgctggcc
       61 gttccagcca gaactccttg aaatagggaa actcttctcc tgtcggcaag cccgtatcga
      121 tggccaagcc gcgcgccgat aaaccgaaca cttggccttt tgccaccgct ctgaagtagc
      181 ttgcaccggc gaagacgacg acgtgattga attcattctt cgcatgaaac ggataggtga
      241 cacgaaaacc ggcaaagcct agatcggcgg ggatcttgtc cgtgaattta tttttaccgt
      301 aggtaaattg ttcaggcgaa aatctcgtcg gccgcaccac cccgccttcg acggtattca
      361 ttttcaccgc atgaccgtaa aacaggcccg gatgtatgta ttgaatttga aagttaccgc
      421 tgtcacgcca caatgaccgc ttggtgtcga agcgaatgtc gcgataatca tcataggaca
      481 gcgatcgcaa aaattccgga ataggttgcg gcggacgaaa cgcttccgcg gcgctcttct
      541 ttgccaaggc cgcgacatcg tcaaattgga accggccgtt tgcttgagcg agggttggta
      601 ataagcaaat aaatgttgtc accaggcgca acatgcattg cagtgcttgc cgcatgctgc
      661 agcaatcttt ccgtgatctg gagaattgcg ctgcagtccg cacgttaaaa ttcaaaattg
      721 ccacaatagt atccggaata ttttaacctt gttcgcgcag atcatagcaa tgaatatgcc
      781 acgatcgcat agaccaactt cggtttcatg ctgaaccgcg ccagagaaaa ttctttttcg
      841 acgtagcgtt cgatccgcat gagttatatc tggaattttg atttcagtga tgctggcttg
      901 ggttcaatgc caacgaaact tgcatactaa accgcaacgc cgagttgaaa actattgtcg
      961 attcagaagg caaatccctt caaccaacaa gtcaatatca gacgccgctg atgcgtgata
     1021 cgttttctct acttaataaa tcgatgactg cggcattaca gttggtgctc aaagatgaga
     1081 aacaacaccg gcccgcagtt tctttacggc atgcctaaca aaacctgacg atgatctgct
     1141 atggcaattg aatctaaacg gactgctgcg attcaccgcg tggcgactgc aaaagcaaac
     1201 tgctaaagaa aagcacaagg gccgcatgct ccttgccctc ggacatcaga cctgtctgag
     1261 cttcactgga aatgctgatc gaacaagctg tcgggaaaag cgtaaatccc tggtcgaccg
     1321 aatcaattgc ggcgcttgga actgactagc atgacatgcg agccgttgtt ccgatcgcgc
     1381 gctgtgccca cgggcgattc ttgcggcggc ttttttcgtt gatttgagtc ttggaaactt
     1441 agttaagtag agtatgtcca gatcgcaaca tgcaacgcaa catcgccccc agttattttg
     1501 aagaattcat gcgacgtttc aggatggtcg cagcaatttt ccctatcgat ttttattgtc
     1561 gtccgagctg cctcaaatag cggcacgcag acgatgaact tatgagcagt gacaagatcc
     1621 gagcactaga actacgctgc ttgcagttgg atgtattcct caacaatttt ggtggtttat
     1681 tgacccggca acctccggtt cgaaaacgct ggattatttc cgattttgct taagacgttg
     1741 tcgctaaccg gtttgtgttc tgcctagttc actgacaata gaatcttgcg cgaacttcgg
     1801 gaagagttca ccggtcgcgg cttggtgcgg gatcgccgtc ggttggcgcg cggccgacta
     1861 ggtgcgacac cgcaacgaaa atatgttgcg tgcccatgtt gacgaccacc acgcgaaata
     1921 gatgaaaagc cacgaccaac ggcacgctga tatgcagcgc ctgggcggta atcgtcatct
     1981 cggctaggcc gccgggtgaa gtggcgatga tcatggtcgc gatcggcagg tcgaatgcca
     2041 aggctagggc gacgccggca atcgccgagg cgatcagaat gaacaatgaa tttagcaggg
     2101 cgaagggaat aaacagcctg tgacgcgcga aaaaagcgcg ttcgtagcgc gcaccgagga
     2161 ccaagccgaa catcagctgg gcgaagtcgg tgagggaatg cggcacggcc gatagttcaa
     2221 caccgcccat agtcaatgcc gcgccgaaaa atatcggcac caacaggcaa ccattgtgaa
     2281 aatgaaatct ttcggaaatc tcgcctaaga cgccactgcc taataaccac accaccaaga
     2341 tcgttggcag caacggtaag tccggcttgt aagcggcggc ttccaaggga atgccgctgt
     2401 aggttaggac gaacggaacg acgatgacga ccaccgagac gcgcaagctg tgcgccaccg
     2461 ccaccggcga catctgcgcg ccatagcggt cggcgagcac cgccatggcc atggcgccgc
     2521 ctgggatcga ggcgaagaag gtcgatttcc agtcgacgcc ggaggctttg ctcaaggtca
     2581 gcgcgccgaa gatgccgacg ataaataccg cgacggtggc ggccaatacc gccgggagat
     2641 tgccggcgag agcggcgacc accggcggcg tgaagtagag cgataccgcc gagcccagta
     2701 tgacctggcc caactggcgg gcatagggcg gtgagtgcat ggtaacgccc atgagattta
     2761 agctcgccac cgcgatcatc ggtcccaaca tccagggtat cggcgtgtgc agccaggcga
     2821 agagaaagcc ggccgggatg ccgactgcca gggcagtcaa gatgccgccg agctgttgtc
     2881 gaaggtgggt aaactcgttc atggtaatcg gatcgatcgg cgctgccgaa tgccgggcgg
     2941 ggaacgcgcg atgagctaga gactcttata gccggtgcgc ggtgggatta cgacctgccg
     3001 gccatttttg ccgctcggtt agcggtggac aggatagtct cagtcgggta aatattcaca
     3061 cctaggagtg agcgtaatag atggactaca aatccgcggt gatcgatgcc gatgcccacg
     3121 tggttgaaag cgaacgggtg tgggactacc tcgaagacag cgagaaaaaa tttcgcccga
     3181 cgttgatgcc gtcgccggaa aatcccaagc ggcaattctg ggtgcttgac ggcgaaaacc
     3241 tcggccccaa gtttccctcc cccgatgagc acgaatcaga agcccatctg aaacgttttg
     3301 gcagggaagt cgggacgccg gtggcagcac gcgagctgtc caacgtgcag caacggctca
     3361 agcacatgga tgagctcggc atcgacgtcc aggtgctgtt caattcgctg tggatcacgc
     3421 cgctcaccag ccgggccgac gccgagattg ctctcagctg ggcttggaat cgctggttgg
     3481 cggatgtctg gcggctcggc gaggatcgct tgcgctggac ctgcgtggtg ccggcgttgt
     3541 cgcccacgga agcggttgca cagattcgct tcgccaaaca gaatggcgcg gtcggcgtgt
     3601 gcatgcgccc attcgaacgg gatcggatga tgaccgatcg gttttactac ccgatctacc
     3661 aagaggcgga tcgtctcgat atcccgatca cggtgcatct ggccaacggt agcccggagc
     3721 ttttcaagct tatgaccaac gaagcaggcg gcggcttttc gaccttccgc gtgcccacgg
     3781 tcacgtcatg ttacgcgctg atcatgagtg aaatcccgca agtgtttccc aaactacgct
     3841 ggggcttcat cgaagtgtcg agccagtggg tgccctgggt gatccatgag gcgatgcgcc
     3901 gttccctagg tagcgcgcgg ccgatctcgc gcgattgttt gcgcgagtac aatatttatg
     3961 tctcgaccca gagcgacgac gattttccct atatactctc ctatgccggc gagggaaacc
     4021 tggtcatcgg cacagactac ggccatggtg acacttccag cgaactcaat gccatctcga
     4081 gattcaaggc gatgacggag ctgagcccgg aggtaaagaa aaagattctc ttcgacaatc
     4141 ccaaagcgct ttacgggatc taggattttc tccggacggg gccgccatgg cgacattaat
     4201 ttcagtcgaa aaagaaaaac agcttgccga acgcatgctc gcactcggcg tgcgcgaagc
     4261 cgatatcgac gagcagttcg tgcgctcttc cggagccggc ggtcagaacg tcaataaggt
     4321 tgcttcttgt gtggtgctcc accacaggcc cacagggctc cgcgtcaaat gccaacagga
     4381 gcgctcccaa gcgctcaatc gctttttggc ccgccgcctc ttactcgata agatcgacac
     4441 ccggcagcgt ggcgcggcga gtgcagagat gcaacgcatc gagaagatcc gccggcagaa
     4501 gcgcaagcgc tcacggcgcg ccaaaatgcg catgctcgac gacaaacacc accagtcgga
     4561 aaaaaaaggt cggcgctcga cggttcggcc ggggcgcgac gactaggttg ctccagcaga
     4621 gtgttgccat cgccatcaag ctgcatccgg cattgtcggg tgccgggaaa attatcagcc
     4681 ttagcgccac ttacttcatc ggagctatcg ccgttcgatt gtcattcttg tcacttttcc
     4741 ccactattgg cactgtcagt gttaccgtcc cgcgagctaa atagttgaaa aataaaagct
     4801 gcggattggc cttagggttg ctgataggca gacctcgagg agcaacacga catgatgaac
     4861 gacctgatct tattggttct ggtggtggtg ctagtgccgc tggcagcgat cgctattttg
     4921 cgcatgctcg gcaagaccaa aagaaccaag cgaccgattc gtgattttcg cgtgcgttga
     4981 agtgaccgct agatttttcc aatcctcgag tttctctcct tgtaatcgac ccgttgccat
     5041 ttagctattc gctcttcttc agctgatcgc gcagtgcctg gatcggcttg aaatccatcc
     5101 attcaggctg gttgataaat aggtatgtct tattgttgtg ccggcgcgag cggcggcaga
     5161 attttctgag gtagattttc gcgcatggag ccaactctca gttcatcgac gttggcttgg
     5221 cggaacgcga tctgaccttc gaacgacaaa aaccgattga gcaagacttt tgcggcgttt
     5281 ggatgggggg tggcgattca tcaggacgat ggctcccgat atgttacatg cggatcatgg
     5341 aaaaattcct ggcgacataa acggcggtta tttcacgcag ttaccgctgg cattactgtt
     5401 gctgaaggac ttgccgggag aaacgctgcg atgatgtatc acatgttcag aaggtcgctg
     5461 atggttgttg ccatgacgtt cggttccttg ttcgcagcgt tgggactaga aaagaaaaga
     5521 aaaaacctgc tggcagttag caggcaccga ccggagcctt gaggcgaagg agctttggct
     5581 aaattatgcc gggcaaacat cattgcctag gtttctcgcc cacttattcg ctctcgttcg
     5641 attgtccaat cctgttattt cttgcactca catcgttcag ttatttgcag cgcggccgtg
     5701 ctacctcgac agcagacggc ggcggctctt ctcgagaccg aagttctcga tgaacttgcc
     5761 gaagacgcgg tcgctaagtt catggagccg gccgatatgg cgcacggtct cggtgtggat
     5821 ccgatcggga tcgatgtcgc cgtggaggct ggcgatctcg cggcggttct ccggtacctt
     5881 gagccaacgc agtatcatcg gttcgtcgac ttcgagatgg aattgaaagg catagacgtt
     5941 ggcaccaaaa cgaaacgctt gattctcgca gtggcgcgac gatgccagat ggatcccccc
     6001 gtggggaatg tcgaaggtgt caccgtgcca ttgaaagagc gtttcccgtt ccttgaaatc
     6061 attgagcagc gggtcatcat cggcgtgggc tgacggcgcg acatcgtacc agccgatttc
     6121 cttgatcggg ttggcgtaga cccgcgcgcc cagggtgcgg gcgaccagct ggccacccaa
     6181 gcaaataccc aacaccggca ggttgcgctt catcgcgctc tcgatcagct tcatctcaac
     6241 agctagatgg ggtagccgct ggtcgtcata ggcggacatg gggcctccta gtacgatcag
     6301 gccagcgtag ccgtccaggt tcggctgggc gtcgggatgg cggccaaaat tcacatagcg
     6361 gatgcgaaag ccggcgtgct ttagcatggg attgagcgtg cccaggatct cgtggggcac
     6421 atgttggcag actaagagtt ttctcatttt cgctgacttt ccagacgaaa atgattttac
     6481 cataaccgat tctcttgacc caaatcggcg cggttaattt tcccgtaa
//
LOCUS       DASQKS010000033         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5898139, whole genome shotgun sequence.
ACCESSION   DASQKS010000033 DASQKS010000000
VERSION     DASQKS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5898139"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VE170_00790"
     CDS             <1..1060
                     /locus_tag="VE170_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ13995.1"
                     /translation="VACTHELSPGQALPIRILSEDFTLYRGAGGTAHLVGNRCAHRGT
                     QLSAGWVEDDCIRCFYHGWKYNGAGQCVEQPAEGESFAAKVKIASYPVREYIGLVFAY
                     LGEGEAPSLPRYPRFESPEISLDVAGLKRICNYFNNVDNSLDNAHVRFVHRRHRDAAE
                     DHVVLGDPIISVEESDWGIRRYVKYPDGKDLTFFFGMPNINFINGQVVDPVIKRADVM
                     VFKVPVDDENHVHFEVRAIALSGERGRAWIEERRALRAKVEEDRPELVRAILAGKLRL
                     SDIDPQRIDFVMLEDEIAQTGQGRIAVRSNEHLGRSDRGVFLLRKIWERELRNLAEGR
                     PLKSWTYRPDMVPTYPEA"
     gene            complement(1148..2134)
                     /locus_tag="VE170_00795"
     CDS             complement(1148..2134)
                     /locus_tag="VE170_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HYJ13996.1"
                     /translation="MAEKKAKKKATGARKRRKLPGKSTGLGAKEVLSAAPPNSVAALQ
                     DAIDQDGGTVLTTYREPFGDHWLIIAALPIDQVEPTPYQRNLSDPHARKLESVIAKVG
                     RFLDPIVTVRKEVKDSPTRYWTPNGNHRLQAMKTLGAKTVIAIVVPEAATAYQILALN
                     TEKAHNLRERALEVIRMYQELATLDDASEESYALEFEEAALITLGLCYLENGRFSGGA
                     YHPILRRSDQFLKKLLGSALEIRRERAKALMDLDKTVIEKVEALKAKGLTSPYLKSFV
                     VARINPIRFRPKDAAPLGFDEVLERMTNAAEKMNVDKIKVEDLAKSGGAADD"
     gene            complement(2300..>2985)
                     /locus_tag="VE170_00800"
     CDS             complement(2300..>2985)
                     /locus_tag="VE170_00800"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ13997.1"
                     /translation="CFLASAVAIGLWIGIHLAGKPADSNSRGLLKDMTEGLSFIRQNE
                     IYWVFIGMTFFNSVFGMSYVIMMPVFARNVLHVGSQGFGFLQSAGGGGALAGVLLAAY
                     LSHFPGKGLQAINGAVVFGVALILFAMSSWFPLSLVAAFALGMAGQFYMTTIHAILQM
                     NLPNELRGRVMGIHGLAWELMPIGGLIAGAIAEFASAPTAVAFGGLMVGGMAFVVAVA
                     MPTIRRLQQ"
BASE COUNT          590 a          842 c          914 g          639 t
ORIGIN      
        1 ggtcgcctgc actcatgagc ttagccccgg tcaggcgttg ccgattcgca tcttgagcga
       61 ggattttacc ctctaccgcg gcgccggcgg caccgctcat ctggtcggca atcgctgcgc
      121 ccatcgcggt acccagctgt cggcgggctg ggtcgaggac gactgcattc gttgtttcta
      181 tcacggttgg aaatacaatg gcgcgggaca atgcgtcgag cagccggcag agggcgaaag
      241 ctttgcggcg aaggtaaaaa ttgcaagtta tccggttaga gaatacatcg gcctggtctt
      301 cgcttatctg ggcgagggcg aagcgccctc gctgccgcgc tatcccaggt tcgaatctcc
      361 cgaaatttcc ctcgacgtcg ccggtctcaa acgtatctgc aactacttca acaacgtcga
      421 caactctctc gacaacgctc atgtgcgttt cgtgcaccgg cgccaccggg acgcggccga
      481 ggaccatgtc gtgctgggcg atccgattat ctcagtcgaa gaatcggact ggggcatccg
      541 gcgctacgtc aaatatcccg acggcaagga tctgactttt ttcttcggca tgcccaacat
      601 taattttatc aacggccagg tggtcgatcc ggtgatcaag cgggctgacg tgatggtttt
      661 caaagtcccg gtggatgacg aaaaccatgt tcacttcgaa gtgcgcgcca tcgcgctttc
      721 tggcgaacgg ggccgggcct ggatcgagga gcggcgcgcg cttcgcgcca aggtggaaga
      781 agaccgcccg gaactggtgc gggcgattct cgccggaaaa ctgcgcctaa gcgatatcga
      841 cccacagcgc atcgatttcg tcatgttgga agatgagata gcccagaccg gccagggtcg
      901 gatcgccgtg cgcagcaacg aacacttggg ccgcagcgac cgcggcgtgt ttctcctgcg
      961 caaaatctgg gagcgcgagt tgcgcaatct agcggaaggc cggccgctca aatcttggac
     1021 ttatcggccg gacatggtgc cgacgtatcc cgaggcgtaa cggggaatta ggtgtaagtt
     1081 gccgacgcga gtctcaagtt cgcgttcctt agctatcatc tggccccgag agaccgctgt
     1141 cgcacagtta gtcatcggcc gctcccccag acttcgccaa atcctcgact ttgatcttgt
     1201 cgacgttcat cttctcggcg gcgttggtca tccgttccaa tacttcatcg aaacccagcg
     1261 gcgccgcgtc cttggggcgg aagcggatcg ggttgatgcg cgcgaccacg aagcttttta
     1321 gataggggct ggtgagccct ttggctttga gcgcttcgac tttttcgatg acagtcttgt
     1381 cgaggtccat caaggctttg gcgcgctcac ggcgaatctc cagggcggag ccgaggagct
     1441 tttttaggaa ctgatcagaa cgtcgcagga tcggatgata ggcgccgccg ctgaagcggc
     1501 cgttttcaag atagcacagg ccgagggtaa tcagcgcggc ttcctcgaac tccagcgcgt
     1561 agctttcttc gctggcgtcg tccaaggtcg ccagctcctg gtacatgcgg atgacttcca
     1621 gggcgcgctc gcgcagattg tgcgccttct cggtattcaa cgcgagaatc tgataggcgg
     1681 tcgccgcctc ggggacgacg atggcgatga cggtcttggc accgagggtt ttcatcgcct
     1741 gcaagcgatg attgccgttg ggcgtccagt agcgcgttgg gctgtcttta acttctttac
     1801 gcacggtgac gatggggtcg agaaagcggc cgaccttggc gatgacgctt tcgagtttgc
     1861 gcgcgtgggg atcggacagg ttgcgctggt agggcgtcgg ttcgacctga tcgatgggca
     1921 gtgcggcgat aatgagccag tgatcgccga acggttcgcg gtaagtggtg agaacggtgc
     1981 cgccgtcctg gtcgatggca tcctgcaatg cggcgacgct attgggcggc gcggcggata
     2041 gaacttcttt ggcacctagc ccggtcgact tgccggggag cttgcgccgc ttgcgcgctc
     2101 cggtggcttt cttcttggct tttttttcag ccattggtgc aagttacagt tttcaattgt
     2161 tcagtttttt taggaacggc agtcttttaa catagtccct acgaatccga ttctatgccc
     2221 tttgggttgt ttgcggccat cgatcttttc ggttgcagcc ataccgcggg agcttcttcg
     2281 tgttggcgat attttcaaac tactgctgta agcggcggat ggtcggcatc gctacggcga
     2341 caacaaatgc catgccgccg accatcaaac cgccgaaggc caccgcggtc ggcgcgctgg
     2401 cgaattcggc gatggcgccg gcgatcaaac caccgatggg catgagctcc caggccaatc
     2461 catgaatacc catgacccga ccgcgcaatt cgttgggtaa gttcatctgc aagatggcgt
     2521 ggatcgtcgt catgtaaaac tgcccggcca tgccgagggc aaaggcggcg acgagcgaga
     2581 gcggaaacca actcgacata gcgaataaga tcaaggcgac accgaacacc accgcgccat
     2641 tgatcgcctg caagcctttg cccgggaagt ggcttaagta ggcggcgagc aggactccgg
     2701 ccagagcgcc gccgccgccg gcactttgca ggaagccgaa gccctgggag ccgacatgga
     2761 gcacgttgcg ggcaaacacc ggcatcatga tgacgtagga catgccgaat acgctgttaa
     2821 aaaacgtcat gccgatgaac acccaataga tttcattttg acgaatgaag ctcagtcctt
     2881 cggtcatatc tttgagcagg ccgcgtgagt ttgaatccgc cggcttgccg gcgagatgga
     2941 tgccgatcca cagcccgatg gccacggccg aagcgaggaa acaga
//
LOCUS       DASQKS010000034         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5898219, whole genome shotgun sequence.
ACCESSION   DASQKS010000034 DASQKS010000000
VERSION     DASQKS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5898219"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1907
                     /locus_tag="VE170_00805"
     CDS             252..1907
                     /locus_tag="VE170_00805"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ13998.1"
                     /translation="MLGGHAVAQCLRNEGVKHIFSVPGESYIAVVDGLVNYPEIQLIT
                     NRQEGCAAFMAEGYAKATRTPGVCIVTRGPGATNASIAVHSAKYDSAPMVLLVGQVSR
                     NARGREAGQEIDYGRFFGSIAKWVIEINDPKQIPRIMSRAFHIARSGRPGPVVVALPR
                     DVTETDTDIAMTEPLPAIKPNADPAAVETLIGRINAARKPVLLIGSGTQYSNAWQEVI
                     DFAEKFQLPVLTSYKRQDAFPNNHPNYAGNLLTSNKQAQNLAYEESDLLIVLGSRLNQ
                     QTTAGFSLPRPGRPFVHIDADEENIGQNSRPEVGIVADAKQLLLAALKHPAPRPNESR
                     ASWIAEQHAAQKQFCTPKERPTPKVSMERVMLDLKTAMPADAITTTDAGSFGQWQQRY
                     LEFDHPDSYISPTLGCMGPGVPSAVAAKLAHPQRMVIAHIGDGGFLMTGQEMATAKQY
                     NAPIIAIVYNNQGYNSIRMHQEAQFPGRQHGVALENPDFALMGESYGALGLKVTRDEE
                     FLPAFKKALAANRSALIEVQTDYEYVTPNARLSELAGKKLAGD"
BASE COUNT          471 a          639 c          595 g          377 t
ORIGIN      
        1 actgtttccg cagttaccac gttggcgaca atagtggcaa gcaccaagtg ccattgactg
       61 gaaacttgca ttgacagccg cgcgccaatc ggactatacg gcaagtaatc attgcggcgg
      121 cagcaaccga aaaatcgtaa gagcggacct gccaccacga aggatggcct gacctttgct
      181 gaatttcgcg tccttggtga cgaaacaaac gagacctggc acagaatcaa ccttgatagg
      241 agatcccatc catgcttggc ggccacgcgg tagctcaatg tctgcgcaac gaaggggtta
      301 aacatatttt cagcgtgccg ggcgaaagtt acatcgccgt cgtcgacggg ctggtgaatt
      361 acccggagat tcaactcatc accaaccgcc aggaaggctg cgccgcgttt atggccgaag
      421 gctatgccaa agcgacgcgc acgcccggtg tctgcatcgt tacccgcggt cccggcgcga
      481 ccaacgccag catcgccgtc cacagcgcga aatacgattc ggcgccgatg gtactgctgg
      541 tcggccaggt cagccgcaat gcgcgcggcc gcgaagccgg ccaggagatc gattacggcc
      601 gatttttcgg cagcatcgcc aagtgggtca tcgagatcaa cgatcccaaa cagatcccgc
      661 gcatcatgag ccgggcattt catatcgcgc gcagcggccg gccggggccg gtggtggtgg
      721 ccctgccgcg cgatgtaacg gaaaccgaca ccgacatcgc gatgaccgag ccgctgccgg
      781 cgatcaagcc caacgccgat ccggcggccg tcgaaacctt gatcgggcgc atcaatgcgg
      841 cgcgtaagcc ggtgctcttg atcggttcgg gaacccagta ttcaaatgcc tggcaagagg
      901 tgatcgactt cgctgaaaag tttcagctcc ccgtgctgac atcgtataag cgccaagacg
      961 cgttccccaa caatcatccc aactacgctg gcaatctatt gacgtcgaat aagcaggcgc
     1021 aaaatctcgc ctatgaggaa agcgacctat tgatcgttct cggcagccgg ttgaaccagc
     1081 agaccaccgc cggcttttcc ttgccgcggc cgggccgtcc tttcgtgcac atcgacgccg
     1141 acgaagaaaa catcggccaa aactcgcgcc ccgaggtcgg catagtcgcc gacgctaaac
     1201 agctgctgct cgcggccttg aaacatcccg cgccgcgccc gaacgagagc cgcgcgtcat
     1261 ggatcgccga gcagcacgcg gcgcaaaagc agttttgcac gcccaaagag cggccgacgc
     1321 cgaaagtgtc aatggaacgg gtaatgcttg atctcaaaac tgccatgccc gccgacgcga
     1381 taaccaccac cgatgccggc agcttcggcc aatggcagca acgctatctc gagttcgatc
     1441 atccggactc atatatttct cccaccctcg gctgcatggg accgggcgtg ccgtcagcgg
     1501 tggcggccaa actggctcat ccccagcgca tggtcatcgc ccatatcggc gacggcggtt
     1561 ttctcatgac cggccaggag atggcgacgg cgaagcagta taacgcgccg atcatcgcga
     1621 tcgtttacaa caaccaaggt tacaactcga tccgcatgca tcaggaagcg cagttccccg
     1681 gccgccaaca tggcgtcgcc ctggagaatc ccgacttcgc cctcatgggt gaatcctacg
     1741 gcgcactcgg attgaaggtg acgcgcgacg aagaattttt gccggcattc aaaaaagcgc
     1801 tggccgccaa tcgttcggcg ctgatcgaag tgcaaaccga ttacgagtac gtgaccccca
     1861 atgcccgttt gtccgagttg gcggggaaaa agttggccgg cgactgacat cggcgggggc
     1921 gaaaaatatt tttcgctctg cccgaatctt cggggcctgc ttaggggcga attgtcggcg
     1981 tgagcagccg gtggtctttc tcaacctatc caagggtgag gacgtcaaag cgtgacataa
     2041 ccgaaagcga ggctctatgc gattcgcctt tcgagtgtcg at
//
LOCUS       DASQKS010000035         8988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_18712, whole genome shotgun sequence.
ACCESSION   DASQKS010000035 DASQKS010000000
VERSION     DASQKS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18712"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VE170_00810"
     CDS             <1..268
                     /locus_tag="VE170_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ13999.1"
                     /translation="PNDGFYIDSLGWVYYQKGDYPRAVEHLERAVELTVDDPTIIEHL
                     GDAYEKAGRVDRAVLRYRESLKHSKEAEQTKRVREKIQRLEKKI"
     gene            265..1086
                     /locus_tag="VE170_00815"
     CDS             265..1086
                     /locus_tag="VE170_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HYJ14000.1"
                     /translation="MTVLPRRSGIVREFAVFVPFALLVFIVAGCATSAKPPALPQPPV
                     PQWESSKLIESLSQRRQQFRSMRALARVNYAGPEGKHGFEEAVLVQRPDRLRLETLTF
                     LGAILIVTANDKEIIGYHPREGVLVRGRSSKDNLLRLTQIPLELEEITALLIGLPPVE
                     VSSPWRQEGNSLIFSVNGQAKDILTFDSQLPVPTRWQLFNGSGMLELTVFFSDYMATP
                     AGLFPSKINVEAPQKGKKLEIRFQEPEFNATIPVESFSQQKPANVQEVPIEMIGS"
     gene            1090..2865
                     /locus_tag="VE170_00820"
     CDS             1090..2865
                     /locus_tag="VE170_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HYJ14001.1"
                     /translation="MFPKSWRFILPVLFIASCGDKTEVKVADVNPAVSKQEAGKPVTG
                     DWLVIHSLSDPEQLNPFTSSDAGSAEVNQYIFESLLTRDPRTLEFKPYIAEARPEISA
                     NKLAYTFKIRRDARFQDGRPVTGADVLFSLKVIKCPLVNAPFLRVYFNSIIDAEMLDP
                     YTIRFVTKEPYFLNESVLGQEIKILPRHYYDPDNLLGNVAIRDLLQEPAKLPEGIKQF
                     ADNFNKNYNRSPLGTGPFKFQRWKTGQEIELVRDVNYWGNGKPGIDQPYLDRLKFRVV
                     NNMDAALVRLKSGGLDYMEVLQPVQHVRGSNSARFNQEFKKYEYYAPAYSYIGWNNVH
                     PIFSDKRVRQAMTYLTDRKQIVKSVLFGLGEVVDSHIYLFRPEYDKSLKSYPFDPKKA
                     SELLHQAGWRDSDGDGILDKEIDGKKTPLRFEIKVNSGNAVRKSIALILLDELKKHGI
                     DVRVRELDWTIFLNDVKNHHFDAVVLGWGMSTTEPDAYQVWHSSQAANKGSNAISYKN
                     PRVDEILEQYRREFDPQKRIQIYKEFQQILHEEQPYTFLYVSKRVSALHRRFQNVEIF
                     PDGLRPIDWWVPARSQKFATTIAAQ"
     gene            2875..3906
                     /locus_tag="VE170_00825"
     CDS             2875..3906
                     /locus_tag="VE170_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14002.1"
                     /translation="MTRYLFQRLLLIIPTFVGITLIVFLIVRLAPGDPAELRAAGGLG
                     AAAGGGISVEKRGAVDEAMAQWRAQYGLDKPLYVQYGVWVKNIFTLSFGISFKDSQPV
                     WDKITERIPVTLKLNFWSLLVVYLVAIPLGIYSATHQNSFIDQATTLIAFVLFAVPLV
                     WAATMALVFICGGDFYYFFPPGGVESLDAAVNWSWWDKVTDQAWHLFLPVVLLSYDGF
                     AGLSRYMRSSMLEVLRQDYVQVARAKGLDEGVVVFKHVLRNSLIPQVTLLASILPGLI
                     GGSVIIETIFSIPGLGQLGFESVLSRDYPVVLALLAVTSILTLIGILISDLLLAVVDP
                     RIAFGRRLQ"
     gene            3914..4966
                     /locus_tag="VE170_00830"
     CDS             3914..4966
                     /locus_tag="VE170_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14003.1"
                     /translation="MASTNLTTQDMVTEITEAGGLWSDVWQRFKRNPVALTGFFVVCF
                     FVFMALFADFIANDKPYYIEYKGKSYFPIFRSYLVGMKLGKWPPELLNVEFKKLEDAK
                     AYFPPIPYRPSNINLLEPLEPPSGRHWFGTDKLGRDVMAGMVHGSRVSLSIGFVAVGI
                     AVMIGVVLGAIAGYFGSWTDLVISRLFEVMLSIPTFFLLITIAALLPPSIFLTMFIIG
                     ATSWVSIARFTRNEFLRIRNLDYVTSAIALGGTDRKVMFKHILPNALAPVIVSVVLGI
                     AGAILVESSLSFLGIGVPAELVTWGSILHEASTTTYAWWLAVFPGFAIFITVLAYYLV
                     GEGLREVLDPRLRGLR"
     gene            5121..6248
                     /locus_tag="VE170_00835"
     CDS             5121..6248
                     /locus_tag="VE170_00835"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ14004.1"
                     /translation="MAERPLEAIKADIAHRAGRINPFERVKREDVEQVLKHLTSLDAE
                     LWGSEWGKFGAKYEALGDDQEKQGNKNNAGESFYLAYEYYRIGRYPVPSSPEKMACYH
                     GALRTFLKAAPYMDPPLERCEIPFEGKKVVGYLQVPKSIDRPPVVMHWGGVDGWKEDR
                     RTNNDVLLKVGIACFTIDMPGAGENPCSGQEPNAERTFSAAMDYLATRSDIDGRRIAC
                     MGGSFGGYWAAKLAHTEPQRLRGAVNWGAGVHRTFQEEWLRPALTLTASQYLMGPASL
                     LDARSYIFGAKSLEEVLKIAPSLSLVTQGLIEQPCAPLLCINGKDDDQHPISDLYLLA
                     EHGSPKDLRIIAGAGHMGRNKGQANDEVVAIVTRWLKEKLT"
     gene            6397..6945
                     /locus_tag="VE170_00840"
     CDS             6397..6945
                     /locus_tag="VE170_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYJ14005.1"
                     /translation="MARITTIDKKEDLKAEDQKIFDAIAQSRGAVGGPWRALLHSPEM
                     AYRTMHLGSYVRFESSLDHKVIEFAALVSARELDCKHEWAAHVNHAQQAGIALDTIRA
                     VYRKQGVETFSSEDAQLVSFVREMIHDHRVSEPTFQAIHGRFGDKGMVELSATIGYYA
                     MLACTLNTFDVYTVTPPDDLKI"
     gene            7030..8724
                     /locus_tag="VE170_00845"
     CDS             7030..8724
                     /locus_tag="VE170_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14006.1"
                     /translation="MGKAAPGDPIGHKIRPARDRLMAKKREPRRQAAPVAAAGELRPA
                     GAFFQVCLTVALLAAYAGFLAHPLELTTTDLGRYLKNGELFFQRGLIVDSNLFAYTTP
                     DYPFVNHSWGSGAIYYLVERSLGFSGLSWFFLLVSVTTLWIFLRLAVRNGSFALGALC
                     AVIVMPILITRHEIRPEMFSYLMSGLFLNLLWAYRQGHRDARWLMILPLLQLVWVNLH
                     IYFFIGMLLVGIFLLQSLIDLAAQSSSGASSQWHPSKVLTVVALATFAASCINPAGLR
                     GALYPLFIFQGYGFPVIENYSVPAILQAGFQFLPLTFFLIIVGVLFLSWLYVIIQDRS
                     RFSLSNFVLSLFFAALAWWMIRNFVIFALFALPLTAANFSTMARAQSWRWLNSPLGTL
                     ATVSGVAVLLVLINPAFFFAGGRGTFGIGLKEGNLAALKFFRSEHLKGPIFNTFDVGS
                     YLTYGLYPRERVYVDNRPEAYPATFFSEEYFPLLVNEEKWRSHLFTNKFNIIVVNPSG
                     HSTAAENFVIRRMLDPDWAPVFFDKEVLILARRFGANQPVIAKHELPREAVLQKTE"
     gene            8827..>8988
                     /locus_tag="VE170_00850"
     CDS             8827..>8988
                     /locus_tag="VE170_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14007.1"
                     /translation="MKLKDQVALITGAGRNIGKSMAKLFAAEGAKIVVAEMHEGRGQA
                     VVDEISKSGG"
BASE COUNT         1862 a         2450 c         2540 g         2136 t
ORIGIN      
        1 gcccaatgac ggtttctaca tcgacagctt gggttgggtt tactatcaaa agggcgatta
       61 cccgcgcgcc gttgaacacc tcgaacgcgc cgtcgagctc acggtggacg atccgacgat
      121 catcgagcat ttgggcgacg cttatgagaa ggcgggccgg gtcgaccgcg cggtgttgcg
      181 ctaccgcgag tcgttgaagc attccaaaga agccgaacag accaagcgcg tgcgcgaaaa
      241 aatccagcgg ctggagaaaa agatttgacc gttctgcccc gccgctccgg catcgttcgt
      301 gagtttgccg tcttcgttcc attcgcgctt cttgtgttca tcgtcgccgg ttgtgccacg
      361 agcgcgaagc cgcctgcatt acctcagcca cccgtgcccc agtgggagtc gagcaagctg
      421 atcgagtcgc tcagccaacg gcgccagcag tttcgctcca tgcgcgcgct ggcgcgggtg
      481 aattatgccg gcccggaggg caaacacggt ttcgaagaag cggtattggt gcagcggccg
      541 gatcgattgc gcttggagac gctcaccttc ctgggcgcga tcctcatcgt caccgccaac
      601 gataaagaaa tcatcggcta ccatccgcgc gaaggcgtgc tggtgcgcgg ccgcagctcc
      661 aaggacaatc tgctgcgtct tacccagatc ccgctggagc ttgaggaaat cacggcgcta
      721 ttaattggct tgccgccggt ggaggtctcg tcgccgtggc gccaggaagg caactcgctg
      781 attttttcag tcaacggcca ggccaaagac atcctgacat tcgattccca attgccggtg
      841 ccgacccgtt ggcagctctt caatggcagc ggcatgctcg aattgaccgt gtttttctcg
      901 gactacatgg cgactccggc cggcttgttt ccatccaaga tcaatgtcga ggcaccccag
      961 aaaggcaaga agctggagat ccgcttccaa gagccggaat tcaacgcgac catccctgtc
     1021 gaatcgtttt ctcagcaaaa acccgccaac gtgcaagaag ttcccatcga gatgatcggt
     1081 agttgagcga tgtttcccaa aagctggcga ttcattcttc ccgtgctttt tatcgcgagc
     1141 tgcggcgaca agaccgaggt caaagtcgcc gatgtcaatc cggcggtgag caaacaagaa
     1201 gcgggcaaac cggtgacggg ggattggctg gtgattcata gcctgagcga ccctgaacag
     1261 ctcaatccgt ttacctcgag cgatgcagga tccgctgagg tgaatcaata tatatttgag
     1321 agtctactga cgcgcgatcc gcgcacgctg gagtttaaac cctatatcgc cgaggcgcgc
     1381 cctgagattt cagcgaacaa actcgcctac acattcaaga ttcgacgcga tgcgcgcttt
     1441 caggatggcc gccccgtgac cggcgcggac gtcttgttca gcctcaaagt gatcaagtgc
     1501 ccgctggtca acgcgccttt cctccgggtt tatttcaatt cgatcattga tgcggagatg
     1561 cttgaccctt ataccattcg tttcgtcacc aaggagcctt actttctcaa cgaaagcgtt
     1621 ctcggccagg agatcaagat cctgccgcgc cattattacg accccgacaa tttactgggg
     1681 aatgttgcaa tccgcgattt attgcaagag ccggccaagt tgcccgaagg cattaagcag
     1741 ttcgccgaca atttcaataa gaattacaac cgaagtcctc tcggcaccgg gccgttcaag
     1801 tttcaacgtt ggaaaactgg ccaggaaatc gagctggtgc gcgacgtgaa ttattggggc
     1861 aacggcaagc caggcatcga tcagccctac ttagatcgtc tcaagttccg ggtcgtgaat
     1921 aatatggacg cggcattagt gcgcttgaaa agcggcggcc tcgactacat ggaggtgctg
     1981 cagccggtgc agcatgtgcg aggcagcaac agcgccagat tcaatcaaga atttaagaag
     2041 tacgaatatt acgcgccggc ttacagctat atcggttgga acaacgttca tccgatcttt
     2101 tctgacaagc gggtccggca ggcgatgacc tacctcacgg accgtaagca aatcgtcaaa
     2161 agcgtcctgt tcggtctcgg tgaagtggtc gatagccata tttacctgtt tcgtcctgaa
     2221 tacgacaaaa gcctgaagag ttatcctttc gatccaaaga aggcttcaga gttgctgcac
     2281 caagccggtt ggcgtgatag tgacggcgac ggcatactcg ataaggaaat tgacgggaag
     2341 aagacgccgc tgcgatttga aatcaaagtg aattccggta atgcggtccg taaaagcatc
     2401 gccctgatac tcttagacga gttaaagaag catggaatag acgtccgtgt gcgcgaactc
     2461 gactggacga tcttcctcaa cgacgtgaag aatcatcatt ttgacgccgt ggtgttaggc
     2521 tggggaatgt cgacgacgga gccggacgcg tatcaggtct ggcattcatc gcaggctgct
     2581 aacaaagggt ccaatgccat cagctacaag aatccgcgcg tcgatgagat actggaacaa
     2641 taccggcgcg agttcgatcc gcaaaaacga attcaaatat acaaagaatt tcagcagatc
     2701 ttgcacgaag agcagccgta cacttttcta tacgtgagca aacgggtctc cgcgttgcat
     2761 cgccgttttc aaaacgtcga gatttttccc gacggtcttc ggcccatcga ctggtgggtc
     2821 ccggcgcgaa gtcaaaagtt tgcgactacg atagcagccc aataagtttt gctcatgacc
     2881 cgatatttgt ttcagagact gctgctgata attccgacct ttgtcggaat caccttgata
     2941 gtttttctaa tcgtccgcct ggcaccaggt gatccggccg agctgcgcgc cgctggcggc
     3001 ctcggcgccg ccgccggtgg cgggatctct gtggaaaagc gcggcgcggt ggatgaagca
     3061 atggctcagt ggcgcgccca gtacggtctc gataagcctc tctacgtgca atacggcgtt
     3121 tgggtgaaga atattttcac tttgagcttt ggcatctctt tcaaagacag ccaaccggta
     3181 tgggacaaaa ttaccgagcg tattccggtg accctcaagc taaatttttg gtcgctcttg
     3241 gtggtttatt tagtggcgat tcctttgggt atttattcgg ctacgcatca gaattccttt
     3301 atcgaccagg cgaccacttt gatcgccttc gtgttgttcg cagtgccgct ggtctgggcg
     3361 gcgaccatgg cgctggtgtt tatctgcggc ggtgatttct attatttctt cccacctggc
     3421 ggcgtggagt ctttggatgc ggcggtcaat tggtcctggt gggataaggt caccgatcag
     3481 gcgtggcatc tgttcttacc cgtggtgcta ctctcctacg acggcttcgc tggattgtcg
     3541 cgttacatgc gctcgagcat gctcgaagtc ttgcgccagg actacgtcca agtggccaga
     3601 gccaaaggcc tcgacgaagg cgtggtcgtc tttaagcatg ttctccgtaa ttcgcttata
     3661 ccgcaggtga ctttgctcgc ttccatcttg ccaggattga ttggcggcag cgtgattatt
     3721 gaaacgattt tttccattcc cggtcttggg caattgggat ttgaatctgt tttatcccgc
     3781 gactatccgg tcgtgctggc tttgctcgcc gtcacatcca tcctaaccct gatcggtatt
     3841 ttgatttcgg atctactatt ggctgttgtc gatccgcgca tcgccttcgg ccgccggttg
     3901 caatgagcca atcatggcat cgacgaattt gacaacccag gacatggtga cggagataac
     3961 cgaagctggc gggctttggt ccgatgtgtg gcagcgcttc aaacgcaatc cggtggcact
     4021 taccggcttt ttcgttgtct gtttttttgt cttcatggca ctgttcgccg atttcatcgc
     4081 caatgacaaa ccttactaca tcgagtataa aggcaagtcg tattttccaa tttttcggag
     4141 ctacctggtg ggcatgaagc ttggcaaatg gccgccggaa ctgctcaacg tcgagttcaa
     4201 gaaacttgaa gacgctaagg cgtactttcc gccaatcccg taccgcccca gtaatatcaa
     4261 tcttctcgag ccgctcgagc cgccgtctgg gcgtcattgg tttggcaccg acaagttggg
     4321 gcgggacgtc atggcgggca tggtccacgg atcgagggtt tcgctatcga ttggttttgt
     4381 tgccgttggt atcgcggtca tgatcggtgt cgtgctcggc gccattgccg gatactttgg
     4441 ctcgtggacg gatttggtca tttcccgttt gttcgaggtc atgttgtcta taccgacgtt
     4501 ttttctattg atcaccatcg cggcgctgct gccgccaagt atttttctca cgatgtttat
     4561 catcggcgcg accagttggg tcagcatcgc gaggtttacc cgcaatgaat ttctgaggat
     4621 tcgtaatctc gattacgtta cctcggcgat tgcgttgggg ggcaccgatc gcaaggtgat
     4681 gttcaaacat attttgccca acgcattggc gccggtcatc gtttccgtgg ttcttggcat
     4741 cgccggggct atcctcgtcg aatcgagttt gagttttctt ggaatcggcg tgccggccga
     4801 gctcgtgact tggggttcga tccttcacga agccagtacc acgacctacg cttggtggct
     4861 ggcggttttt cccggttttg ccattttcat caccgtactg gcttactatc tcgtcggcga
     4921 aggattgcgc gaagtgctcg atccacgcct gcgggggttg cgctagccgc gctctttctg
     4981 ttaagtcggt cttaattctt tcagacgttc ccataggagc acgtccacgt ggcgcgtgat
     5041 ccgcgtagct tcctcggcgt cggcgcgaga tggatccgtg gactcatcga accgtcgagc
     5101 caagctcgag gagcaacctc atggcggaaa ggcctctcga agcaatcaaa gcggatatcg
     5161 cgcatcgcgc cggacggatc aatccgttcg agcgagtgaa gcgagaagac gtcgagcaag
     5221 ttctgaagca tctaaccagt ctggatgccg agctttgggg aagcgaatgg ggcaagttcg
     5281 gcgccaagta cgaagcgttg ggcgatgacc aagaaaaaca gggtaataaa aataacgccg
     5341 gcgaaagttt ctatcttgcc tacgagtatt atcggattgg ccgatacccg gtgccgagca
     5401 gcccggagaa gatggcctgc taccacggcg cgctgcggac ttttctcaag gccgcgccct
     5461 atatggaccc gcccctggag cgttgtgaga ttcccttcga aggcaaaaaa gtcgtcggct
     5521 atctgcaagt gcccaagagt atcgatcgac cgccagtcgt catgcactgg ggcggcgtcg
     5581 acggctggaa agaagaccgc cggaccaaca atgacgtatt gctcaaggtc ggcatcgcct
     5641 gctttaccat cgacatgcct ggtgccggcg agaatccctg ctcggggcag gagccaaacg
     5701 ccgagcggac cttttcggcg gcgatggatt atctggccac ccgcagtgac attgacggca
     5761 ggcgcattgc ctgcatgggc ggcagcttcg gcggctactg ggcggccaag ctcgcgcaca
     5821 ccgagcctca gcggctgcgc ggcgcggtca actggggcgc cggcgtgcac cggacctttc
     5881 aggaagagtg gctgcgcccg gcgctgactt tgaccgcgtc gcaatacttg atggggccgg
     5941 cgagcttgct cgatgcacgg tcgtatattt tcggcgcaaa gagcttggaa gaagtgctaa
     6001 aaatcgcgcc gagcttgtcg ctggtgaccc agggcttgat cgagcaaccc tgcgcgccgc
     6061 ttctgtgcat taacggcaag gacgacgatc agcatccgat cagcgactta tacctactgg
     6121 cggagcatgg cagcccgaag gacctacgca tcatcgctgg cgcgggccat atgggacgca
     6181 ataaaggtca ggcgaacgac gaggtagtcg ctatcgtgac ccggtggctc aaagaaaaat
     6241 tgacttaggg caatttgctg ttgaccacgc tgcgcgcgag tgagaaccag cgacagctgg
     6301 gattcccaat aaagcgacag cccgcaatgg cgaatttggc ctaattattt tgaaatcggc
     6361 aatctgatat ctgtaattct gaaacggagt gagaatatgg cacgcatcac aacgattgat
     6421 aaaaaagaag accttaaagc cgaggatcaa aagattttcg acgccattgc gcagagccgc
     6481 ggcgcggtgg gcggtccgtg gcgagcgctc ctgcacagcc cggagatggc gtaccggacc
     6541 atgcatttgg gcagctatgt gcgtttcgag tcgagcttgg atcacaaggt catcgagttc
     6601 gccgcattgg tttcggcgcg ggagctcgac tgcaagcatg aatgggccgc ccatgtgaac
     6661 cacgcgcaac aagcgggcat cgccctcgac accattcgcg cggtctaccg aaagcagggc
     6721 gtcgagacgt tttcctcgga agatgcccag ctcgtcagtt ttgtccgcga gatgatacac
     6781 gatcatcgag tgagcgagcc gacttttcag gcgatccacg gccgcttcgg cgacaaaggc
     6841 atggtcgagt tgtccgcgac catcggttat tacgccatgc tcgcctgcac gctcaacacc
     6901 ttcgatgtct acaccgtcac gccgcccgac gatttgaaga tctgagcgga tgcccgccgc
     6961 ggggtcgtcg tcgttcaaga tcagcttaat tatctgcggc gacgacggcg gctctcgagc
     7021 cgctgaagcg tgggaaaagc agcgcccggc gacccgatcg gacataaaat ccgccctgcg
     7081 cgtgatcgat tgatggccaa gaagcgtgaa ccgaggcggc aagccgcgcc agtggccgcc
     7141 gcgggtgaac tccgtccagc gggcgcgttc tttcaagttt gtctgaccgt tgctttgctc
     7201 gcggcctacg ccggctttct cgcgcacccg ctcgagctga ccaccaccga cctgggccgc
     7261 tatctgaaga acggcgaact tttctttcag cgcggtctta tagtcgacag caatcttttt
     7321 gcctacacga cgccggatta tccgttcgtc aatcattcct ggggcagcgg tgcgatatac
     7381 tatctcgtcg agcgttcgtt gggcttttcc gggctatcgt ggttttttct tcttgtcagc
     7441 gtgacgaccc tttggatttt cttgcgcctg gccgtgcgca acggcagctt cgctctcggc
     7501 gccctatgcg ccgttatcgt catgccgatt ctaatcaccc gccacgaaat ccgaccggaa
     7561 atgttcagtt atctcatgag cggcctgttt ctcaatctgc tgtgggctta ccggcaaggc
     7621 caccgtgacg cgcgttggct gatgattcta ccgctgttgc agcttgtttg ggtcaatttg
     7681 cacatttact ttttcatcgg catgttgctc gtcggtatct ttctcttgca gtcgctcatc
     7741 gatctcgccg cccaaagctc gtctggcgcc agttcccaat ggcatccctc gaaagttctc
     7801 accgtggtag cgctggcgac ttttgccgcc agttgtatta acccggcggg cttgcgcggc
     7861 gcactctatc cgctgtttat tttccagggc tatggattcc cggtcatcga aaactattcg
     7921 gtgccggcaa ttcttcaggc aggcttccag tttcttccgc tcactttttt cttgatcatc
     7981 gtcggggtgc tctttttaag ttggctttac gttatcattc aagaccgttc tcgtttctcg
     8041 ctgagcaact ttgttctatc gttgtttttc gccgcgctgg catggtggat gatccgtaat
     8101 ttcgttatct ttgccttgtt cgccttgccg ctgacggcgg ctaactttag caccatggcg
     8161 cgagcgcaat cgtggcgctg gctaaattcg cctctcggca ctctggctac ggtgagcggt
     8221 gtggcggtgc tactcgtgct gatcaatcct gcattttttt ttgccggcgg tcgcggcact
     8281 tttggcatcg gcctcaaaga gggaaatctg gcggcactga agtttttccg cagcgaacat
     8341 ttaaaggggc cgatcttcaa caccttcgac gtcggcagtt atctgaccta tgggctttat
     8401 ccccgcgagc gcgtgtatgt cgacaaccgc cctgaagcct atccggcgac atttttctcc
     8461 gaggaatatt ttccactcct ggtgaacgag gaaaagtggc gcagtcatct gtttaccaat
     8521 aaattcaaca tcatcgtagt caatcccagt gggcactcca cggcggcgga aaattttgtt
     8581 attcggcgca tgctcgatcc ggattgggcg ccggtgtttt tcgacaagga agttctgatt
     8641 ctcgcccgcc ggttcggcgc caaccagccg gtcattgcca agcacgaatt gccgcgcgaa
     8701 gcggtgctgc aaaaaaccga gtgagggaaa gtttgagcgt tagttgacac actgtcaggg
     8761 cgtcggatag gctcggtgcg attgcgcggc atattacgcg cgagaatatt taaaagggga
     8821 atgtaaatga aactgaagga ccaagtggcg ctgattaccg gggcgggccg aaacatcggc
     8881 aagtctatgg cgaagctgtt cgcggccgaa ggcgcgaaga ttgtcgttgc cgagatgcat
     8941 gaggggcgcg gccaagccgt ggtcgacgag atcagcaaat ccggcggc
//
LOCUS       DASQKS010000036         5134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1279371, whole genome shotgun sequence.
ACCESSION   DASQKS010000036 DASQKS010000000
VERSION     DASQKS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279371"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2205
                     /locus_tag="VE170_00855"
     CDS             217..2205
                     /locus_tag="VE170_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ14008.1"
                     /translation="MTQQLRLSSAPLRQAICGFLALLLTGFANVARAQTVAPVANDIG
                     ALKNLPFEELFNIAITTVSKRPERLVNAASAIQVITQEEIRRSGASSLAEALRLASNL
                     QVAQVDSRQWAISARGFNSTTANKLLVLIDGRTVYTPLFSGVFWDVQNVMLEDIDRIE
                     VISGPGATLWGANAVNGVINVITKTARETQGALVSAGAGSMLRGFGNARYGDKVGSDG
                     YFRVYGMGFDRDNTFLRSGREGINNWYIGQGGFRGDWMPANGAKVSIQGNFYGGEIAQ
                     PVPGDLTVDGQNLLGRWTRTLANNSEISLQAYWDRTSRRIPFSIAEVLNTYDVEFQHR
                     LPIGERNNFIWGGGYRFMHDDVNNNAAIAFRPTRRDLQLVSGFLQDDISLIRDQLILT
                     VGSKFEHNAFSGFEFQPSARIAWLPVTNHTLWAAVSRAVRSPSRIDTEVFAPSAPPFL
                     LRGGGNRFDSETVIAYELGYRTELANRLGLALSTFFNDYDEIRSVEPVAGAPGQFIIL
                     NGLQAKTYGAELSLAWQATDWWRLRGGYTYFRKRIHFGDSQDINRGRGEGNDPHHQLL
                     LQSMINLPGNLELDSVLRYVDNLNQVGPTVPSYVSLDLRLGWRPTPNWEFAIVGQNLL
                     DKKHAEFGAPATRQEIPRSVFGKLTWKFWTGGNDAKIQ"
     gene            2186..2827
                     /locus_tag="VE170_00860"
     CDS             2186..2827
                     /locus_tag="VE170_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYJ14009.1"
                     /translation="MTRKFNNDDFACEDSPAAQPGFVNLSVRVAATGNFRQRVFWAVF
                     VFAYFVLTAPAVAQVSKEYQLKAVFLWRLAQFTQWPSDVFETSDSPIVICVLGDNPFD
                     GALNAAVAGETAHGRKFVVQNHRAVEQLKTCHILYMAAAGPRQAKEITAALARKSVLT
                     VRESDIASSYQTIVQFLTEQNKIRLQINLKAAAAARLVLDPRLLRSAEIIETE"
     gene            2824..4641
                     /locus_tag="VE170_00865"
     CDS             2824..4641
                     /locus_tag="VE170_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ14010.1"
                     /translation="MTPGSEPKKSLQKRVRAVILLASVIVLFVTAGAFVTYEAVSFQY
                     RLVRNLSTLARVIADNSAAPLAFDNKAVADEILAALRAEPDIEAAAIYDGRGNVFAKY
                     PPALQPAALPARPGRPGYEFKNNGLVLFEPITQEVKQIGTLYLQSSFRGLYEQLWRYG
                     LIVLAVLCGAFIAALILSTLLQKRISGPILALTHAAETITQRGDYSVRAPKLTDDELG
                     TLTDAFNRMLSETQDNQDRLAEQARLLDLSTDAIIVRDLQGTIRFWNRGAEELYGWSQ
                     SEALGKQKHQLLQTEFSEPLEQITDRLQREGRWAGELVQRRRDGGRIHVSTRWVLDSG
                     MAGRLPRVLITDNDITLRKQAEELMRSEAKRLDALVAQRTARLQETVGELEAFSYSIS
                     HDMRAPLRAMQGYANALLSDYGARLDADARHYLDRIFRSANRLDLLIQDVLSYSRVAK
                     GQIDLQPVDFERLLNDVISTTPEFQEPQARIIVERPLHRVRGHDAYLTQCITNLLANA
                     VKFVPERVVPEIRIRSERFNGQVRVWFEDNGIGIDPLHNERIFQIFGQVYPEKKYGGT
                     GIGLAIVSKAVQRMNGEVGVISGLDKGSRFWLLLNHGDT"
     gene            4638..5078
                     /locus_tag="VE170_00870"
     CDS             4638..5078
                     /locus_tag="VE170_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ14011.1"
                     /translation="MTTHRAILLVEDNEDDVFLMKRALKAARVMNPLYVVEDGQEAVD
                     YLGGAGKFANRESYPLPAVVFLDLKLPLISGHDVLAWIRRQYALESVVVIVLTSSNEA
                     SDLSRSYALGANSYLVKPPTPEQLEELAKAFKWYWLEYNQFAND"
BASE COUNT         1099 a         1447 c         1498 g         1090 t
ORIGIN      
        1 ctgagcatct gcgtccacgc caggtcctgc gctttatcac ggccgagcat ttgtcgatca
       61 gcattctccc ggcacaacag ctaagcctgt aattgacaaa agaccgcggc gttcgctatt
      121 tggaatcagt taaattaaaa ctgcagcatc tgccgtcgcg gtgcttcgta aagatgcgca
      181 ttttcgcagc ggcattagcg ggccagaggt aaaactatga cgcagcagct tcgactgtcc
      241 tctgctccac tgcggcaagc aatctgcggc tttttggcgc tgctattgac gggctttgcc
      301 aacgtagcaa gggcgcagac cgtggcgccg gttgccaacg atatcggggc gctaaaaaac
      361 ctgccatttg aagagttatt caacatagcc atcaccaccg tatcaaaacg cccggagcgt
      421 cttgtcaatg ctgcttcggc tattcaagtc atcactcagg aagaaatccg gcgctccggc
      481 gcgtcgagtc tggccgaagc actgcgtttg gcgtccaatc ttcaagtggc gcaagttgat
      541 tcgcgccagt gggcgattag cgcccgcggt ttcaacagca cgacagccaa taagctgctt
      601 gtcctaatcg acgggcgcac cgtctacacg ccgctttttt ccggagtctt ctgggacgtc
      661 cagaacgtca tgctcgagga catcgaccgc atcgaagtga tcagcggtcc gggagcgacg
      721 ctgtggggcg ccaacgcggt caatggtgtc atcaacgtca taaccaagac ggcgcgcgaa
      781 actcaaggcg ccctggtgag cgcgggcgca gggtcgatgc tcagaggctt cggcaacgcg
      841 cgctacggcg acaaggttgg cagcgacggt tacttccgcg tttacggcat gggctttgat
      901 cgcgacaaca cgtttttgcg tagcgggcgc gaagggatta acaactggta tatcggacaa
      961 ggcggctttc gcggcgactg gatgcccgcg aatggcgcga aggtcagtat tcaggggaat
     1021 ttctacggcg gcgaaattgc ccagcccgtg ccgggcgatt tgacggttga cgggcaaaat
     1081 ctgcttggcc gctggacgcg cactctcgcc aataattccg agatctcgct gcaggcatat
     1141 tgggacagaa ccagccgccg gattccattc tcgatcgcgg aggtgctgaa cacctacgac
     1201 gtcgaattcc agcaccgcct gccgatcggc gagcgcaata attttatatg gggcggcggc
     1261 taccggttca tgcacgacga cgtgaataat aatgccgcga ttgcctttcg gccgacgcgt
     1321 cgcgatttgc agctcgtcag cggcttcttg caggacgata tttccctgat tcgagatcaa
     1381 ctgattctga ccgttggttc gaagttcgag cacaacgcct tttcagggtt tgagttccag
     1441 ccgagcgcgc gcatcgcttg gctgccggtc accaaccata cgctgtgggc cgcggtgtca
     1501 cgcgccgtgc gatcaccgag ccgcatcgac accgaagtgt ttgccccgag cgcgccgcct
     1561 tttttgctgc gcggcggcgg caatcgcttc gattcggaga ccgtgatcgc ctacgaactc
     1621 ggctaccgga cggaactggc aaatagactg ggtctggcgc tttcgacttt tttcaacgac
     1681 tacgatgaga ttcgcagtgt cgagccggtc gccggcgcgc ccgggcaatt tattatcctg
     1741 aacgggttgc aggcgaaaac ctatggggcg gaattgtcgc tcgcctggca ggccacggat
     1801 tggtggcgac tgcgcggcgg ttacacctat ttccgaaaac gcattcactt cggcgatagt
     1861 caggacatca atcgcggccg cggcgagggc aacgatccgc atcatcagtt gctgctacag
     1921 tcgatgatca atctgccggg taatctggaa ttggactccg tgctgcgtta tgtcgacaac
     1981 ctcaatcagg tcggcccgac ggtgccgagt tacgtctcgc tggacctgcg cttgggctgg
     2041 cggccgactc ccaactggga attcgccatc gtcgggcaaa accttttgga caaaaaacat
     2101 gctgaattcg gcgcgccggc cacccgccag gaaatcccgc gcagcgtttt cggcaagctg
     2161 acatggaagt tttggaccgg cggcaatgac gcgaaaattc aataacgacg acttcgcttg
     2221 cgaagactcg cctgcggcgc agcccggttt cgtcaacctg agcgtgcggg tcgcggcaac
     2281 tggcaatttt cgtcagcgcg tgttctgggc agtttttgtt ttcgcctatt ttgttttgac
     2341 tgcgcccgct gttgcgcaag tctccaaaga atatcaattg aaggcggttt tcttgtggcg
     2401 cttggcccaa tttacccagt ggccgagcga tgtttttgaa acttcagaca gccctattgt
     2461 catttgtgtt ttaggcgaca acccgttcga tggcgcgttg aatgcggcgg tagcggggga
     2521 aacggcgcat ggccggaaat tcgtcgtaca aaatcaccgc gcggtggagc aactcaagac
     2581 ctgccatatc ctttacatgg ccgccgccgg tccgcgtcaa gccaaggaaa tcaccgccgc
     2641 gctggcgcgc aaaagcgtgc ttaccgtgag agaaagcgac atagcgagtt cgtatcagac
     2701 gatcgttcaa ttcctaaccg aacaaaataa gatcaggctg cagatcaatc tcaaggctgc
     2761 cgcggccgcg cggctggtgc tcgacccgcg gctgctgcgt tcagcggaaa taattgagac
     2821 cgaatgacgc ccggatccga gccgaagaaa tcactgcaaa aacgcgtgcg cgcggtaatc
     2881 ctgctcgcca gcgtcatcgt tttatttgtc accgccggcg ctttcgtgac ctacgaagct
     2941 gtcagctttc aataccgcct ggtccgcaat ctttccacac tggcacgggt gatcgcggac
     3001 aacagtgccg ccccgctggc cttcgacaac aaagcggtgg ccgacgagat cttggccgcg
     3061 cttcgagccg agcccgatat cgaggctgcg gctatttacg atgggcgcgg caatgttttt
     3121 gcaaaatatc cgccggcgct gcaaccggct gcgctgccgg cgcgccctgg tagaccggga
     3181 tacgaattca aaaataacgg gttggttctt ttcgagccaa taactcaaga agtaaaacag
     3241 atcggcacgt tgtatctgca aagttctttt cgcggcctgt acgaacaact gtggcgttac
     3301 ggcctgattg tcctcgccgt tctatgcggc gcttttatcg ccgccttaat cctctcgacg
     3361 ttgctacaga agcggatttc cggtcccatt ttggcgctca ctcatgccgc ggagacgatc
     3421 acacagcgcg gcgattactc ggtgcgcgcg cccaaactga ccgacgatga gctcggcact
     3481 ttgaccgacg ctttcaaccg aatgctttct gaaactcagg acaaccagga ccgcctcgcc
     3541 gagcaggcgc ggctgctgga tctaagcacc gatgcgatta tcgtgcgcga cttgcagggc
     3601 accatacggt tttggaatcg cggcgcagag gagctttacg gctggtccca atcagaagcc
     3661 cttggcaagc aaaaacatca attacttcaa acggaatttt ccgagccgct ggagcaaatc
     3721 acggacaggc tgcagcgaga ggggcgctgg gccggcgaac tagttcagag gaggcgcgac
     3781 ggcggccgga ttcacgtcag cacccgctgg gtcctggact caggaatggc gggccgtttg
     3841 ccaagagtgt tgatcaccga caacgacatc acgctgagaa agcaagccga ggaactgatg
     3901 agatcggaag ccaaacggct cgatgccttg gtagcgcagc gcaccgcccg gctgcaagaa
     3961 accgttggcg agttggaagc gttctcgtat agcatctcgc acgacatgcg cgcgccgttg
     4021 cgcgcgatgc aaggttatgc aaacgcgttg ttgagcgatt acggtgcaag gctggatgcg
     4081 gacgccaggc attatcttga cagaatcttt cgctccgcca atcgattgga tctgctgatc
     4141 caggatgtgc tcagctacag cagggtcgcc aaaggccaga tcgatctaca gcccgtggat
     4201 tttgagcggc tcctcaacga cgtgatttcg acgactccgg agtttcagga gcctcaggca
     4261 cgcatcatcg tcgagcggcc gctgcaccgg gtgcgtggac atgacgctta cttgactcaa
     4321 tgcatcacca acttgctcgc caatgcggtc aaattcgtac ccgagcgagt ggtgccggaa
     4381 atccgcattc gcagcgagcg gttcaatggc caagttcgcg tgtggttcga agacaatgga
     4441 atcggcatcg atcccttgca caatgagcgc atttttcaga tattcgggca agtctatccg
     4501 gagaaaaaat acggcgggac aggtatcgga ctggcgattg tgagcaaagc cgtgcagcgc
     4561 atgaacggcg aagtgggagt gatttcgggg ttggacaagg gcagcaggtt ttggctgctg
     4621 ctgaaccatg gtgacacatg acgacgcatc gggcgatctt actggttgaa gacaacgaag
     4681 acgatgtgtt tctaatgaag cgcgcgctca aggccgcgcg cgtcatgaac cctctctacg
     4741 tcgtcgagga cggccaggaa gcggtcgatt acctcggcgg cgcggggaaa ttcgccaatc
     4801 gcgaaagcta tccgttgccg gccgtggttt ttctcgacct caaattgccg ctcatttcgg
     4861 gacacgacgt gctcgcgtgg atccgccggc aatatgcttt ggagtcggta gtcgtgatcg
     4921 tgctcacctc ttccaacgaa gccagcgact tgagccgatc atacgcactc ggcgcgaact
     4981 cctatctggt caagccgccg acgccggaac aactggaaga gctggccaag gcgttcaagt
     5041 ggtattggct cgaatacaat caatttgcca acgattgacg atgcgcagtt tcattgaggg
     5101 tgttcgttga ccgagatctc ccgcagacac tttt
//
LOCUS       DASQKS010000037        12609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2119162, whole genome shotgun sequence.
ACCESSION   DASQKS010000037 DASQKS010000000
VERSION     DASQKS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119162"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VE170_00875"
     CDS             <1..1101
                     /locus_tag="VE170_00875"
                     /inference="COORDINATES: protein motif:HMM:NF036694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HYJ14012.1"
                     /translation="ILPLKGKILNVEKARFDKMLSSQEIRLMITALGTGVKEDYDQAK
                     LRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPELVEKGYLFIAQPPLYKIKKGKQERY
                     LKDEAMLEDHLIDLGTEGVRVRAGQNGNGNGNGLVGQPLQSFVKKIARWEKLLISIGR
                     KRKNRTVVEALLLAEGFGEEMLKDRQALQDLESRLATYVGLAAPEQAPLSCALEEDAE
                     HNCFKLVVTTRANGTGYQTVIDREFLISAEFREIKKLFGDLSAIGVPPYAIEDGDAKA
                     QLKSFSAILSYVMEGGKRGQYIQRYKGLGEMNPEQLWETTMNPETRVLLQVRVEDAVE
                     ADLIFSTLMGDEVEPRRKFIEDNALSVRNLDI"
     gene            1182..3641
                     /gene="gyrA"
                     /locus_tag="VE170_00880"
     CDS             1182..3641
                     /gene="gyrA"
                     /locus_tag="VE170_00880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HYJ14013.1"
                     /translation="MEATIKPQKVPVYIEEEMRESFMAYAMSVIISRALPDVRDGLKP
                     VHRRVLYAMYDASNTSDKPYKKSARLVGDIMGKYHPHGDTAIYDTVVRMAQDFNLRYP
                     LVDGQGNFGSIDGDNPAAMRYTEIRMTLLAEEMLADIEKDTVDFIANYDDSLKEPSVL
                     PSRIPNLLINGSAGIAVGMATNIPPHNLSEVIDALVALVDNPELTIKQLMRHIPGPDF
                     PTGGFIHGRDPIVRAYHEGKGIVQMRGKAFTETVKRTGKEQIIIREIPYQVNKKRLIE
                     QIAELVNDKKIEGISDLRDESDRDGMRIVIELKRDAVAEIIINQLHKHTQLQESFGMN
                     MLAIVDGKPKLLTLKEALKCFLDHRKEVITRRTAYDLRKAEERLHILDGFRIALDNLD
                     AVIALIRKSADGKEAKEGLMSNFALSELQAQAILDLRLQRLTGLERDKIMAEHRETVE
                     LIAKLRAILADEKEIYKIIVGELKEIKKQFGDERRTQIVDHSEDISIEDMIVDEDMAV
                     TISHEGYIKRNPVTLYRAQRRGGKGKIGTTTKEEDFVESLFIASMHSYILFFTTVGKV
                     YWIKVHELPQASRAARGKPIVNLLNIEPDEKVSAFLSVREFQEGRFIIFATKNGLIKK
                     TELMAYAHPRPSGIRAIGLEDHDEVIGVRLTDGQQELILSTAEGQSIRFKEEQVRATG
                     RGTFGVVGMRLDEGDHVVSMEILTRGFAVLTVSEGGYGKRTEMDEYRLQSRGGKGIIT
                     MKTSDRTGRVVGVQQVTEEDQLMLVSNIGKIIRLRIKDIRIIGRNTQGVRLIELEEGE
                     RLVSLARVAEKEEEDDGGGDA"
     gene            3850..4857
                     /locus_tag="VE170_00885"
     CDS             3850..4857
                     /locus_tag="VE170_00885"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYJ14014.1"
                     /translation="MAREIGVIGAGGWGTALAKVLADKGEQVTLWCHGAASFCELTEH
                     RQNFTYLPGVALPEKLAVTQVLKNAVAGKSLVICAVPSHAVREVFTDAAAYLDGQTHL
                     LCGTKGLEETTLHTMAELFADIFGTASKGRHGFLSGPTFASEVAQGLPAAVTVASTSE
                     SVARSIQGILSTASFRVYTSLDVVGVQIGGVVKNVIAIAAGISDGLGLGQNARAALIT
                     RGLAEIIRLAVRMGADPATLAGLPGLGDLVLTCAGDLSRNRAVGLQIAAGKSLSEITA
                     STRTVAEGVRNTRALHALAQRLAVEMPIVEQMYQVLYAAKRPDEAARDLMQRSLKPEA
                     G"
     gene            4935..5099
                     /gene="rpmH"
                     /locus_tag="VE170_00890"
     CDS             4935..5099
                     /gene="rpmH"
                     /locus_tag="VE170_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYJ14015.1"
                     /translation="MSKRTYQPSNIRRKRTHGFLARMATPGGRNVIKRRRAKGRKKLT
                     VSVPPKRART"
     gene            5115..5474
                     /gene="rnpA"
                     /locus_tag="VE170_00895"
     CDS             5115..5474
                     /gene="rnpA"
                     /locus_tag="VE170_00895"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYJ14016.1"
                     /translation="MKDNQAFPKSARLRKRREFLRLSRSGYKIHSANFVVISKTSDHA
                     ESRVGITVSGKVGNSVIRNRIKRQVREFFRRRRAALPQAVDFLVIARKSAVNLPSSMV
                     DVELAESFSPPRGRRMV"
     gene            5479..5691
                     /gene="yidD"
                     /locus_tag="VE170_00900"
     CDS             5479..5691
                     /gene="yidD"
                     /locus_tag="VE170_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYJ14017.1"
                     /translation="MIRWLLILPIGAYRLLVSPWLPPACRFYPSCSDYAQTAIHRHGA
                     VKGLLYTLWRFCRCHPFSAGGYDPVS"
     gene            5701..7344
                     /gene="yidC"
                     /locus_tag="VE170_00905"
     CDS             5701..7344
                     /gene="yidC"
                     /locus_tag="VE170_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HYJ14018.1"
                     /translation="MDKRALIGIALSVLVLVGYQEFITRYYGPPPTVPAPAAEKTESA
                     PPANTVSPSGTTPAAPSAMPAQVAPAIVPPSQAARNVQIETDNYIAVFTSHGARLKSF
                     QFKHYRASAAPNSPLQEMITTAKDVPLPLGVRWQAPAPFDDAGLAYSVQGADLKLTGE
                     AKGTLVFQGQTADGVTITKTLGFTGNLYPIQFDVTVQSAAGNAPVPEILLTDKADHTV
                     PHPGAPFEGFVALVDNKIKREATVDASSGYEFSGDVAWAGLGYTYFFVALMPENGAGH
                     KVSVRLNGAALIATVSGPAATDRYRLFIGPKELELLKSVGKDIDRAIDHGYFGFISVP
                     LLYVLHFFHRFTGSYGVDIIILTVLIKLIMWPLTHKSFSSMKSMQKLGPQMEKLKERY
                     ANDKEKLNKEIMDLYKRNGVNPLGGCLPMALQFPVFIGLYNALSTPIELRHAPFVWIK
                     DLSRPDWEALQFAVPVWWPSFLPSGIPILTIVMGATMFIQQWMTPSAGDPNQRRMMML
                     MPLMFTFMFVSFPAGLTVYWLVNNVLSIAQQYWINRSDK"
     gene            7361..8488
                     /gene="jag"
                     /locus_tag="VE170_00910"
     CDS             7361..8488
                     /gene="jag"
                     /locus_tag="VE170_00910"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HYJ14019.1"
                     /translation="MDFVETEGDNIDAAIENALKALGVGREKITIDIISEGRKGILGF
                     GAQKAKVRASLRKLNLEADPAPAKPQAAPVQQAPARAAEPRPRPPERRPAEPRQAQPS
                     RSEPRNRAPDPRPRAPEARPRSEPRPTPVPTPRPPEEPQAADAPPPLTPAEVMAMVVK
                     AKTALVEILNLMGMNATVTEKAGIDSEETILEVKSENSGLIIGRKGQTLEALQYLVTR
                     IAGERPGNDAPHVVIDIESYHERRRQTLQDMALRLGEKAKRQRKTVTIDALSAADRKV
                     VHAALQDDPWLTTRSLGQGAYRRLLIIPEGDRKIRDEGPAPAQEREGGRPQRSSRSNS
                     PPGDSRSPMRQFGGQERPGGDNPEDPEIAGDKDDSESKGGT"
     gene            8610..9707
                     /gene="ispG"
                     /locus_tag="VE170_00915"
                     /gene_synonym="gcpE"
     CDS             8610..9707
                     /gene="ispG"
                     /locus_tag="VE170_00915"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYJ14020.1"
                     /translation="MIERRKTKQIELGKVKVGGDAPITVQSMTKTDTRDVRATVQQIW
                     SLEAAGCEIVRCAVPVREAAEQLGEIKKKIRIPLVADIHFNYKLALIAIEQGVDGLRL
                     NPGNIGARKYVEEVVKAASERKIPIRIGVNAGSLEKDLLQKYNGPTAAGMVESGLRHI
                     HILEDVGYSEIKISLKASDPLMMIEAYRMLADQVNYAFHLGVTEAGTPGIGTIKSAVG
                     LGTLLAEGIGDTIRVSLAADPVEEVRVGLEILKALKLRKDGMTFVACPSCGRADVDLV
                     ALAKAVEERMLPYSNHNIHVAVMGCEVNGPGEARAADLGVAGGKGIGLIFKRGEVIRK
                     VPEAQIVDALMEEVVKFIAEQPAAAATAAPS"
     gene            9753..11480
                     /locus_tag="VE170_00920"
     CDS             9753..11480
                     /locus_tag="VE170_00920"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HYJ14021.1"
                     /translation="MRWNKTLIPTLKEDPADAEVISHKLLVRAGYIRQISRGIYDYFP
                     LALKVIRKIETIVRQEMDRAGAQELLLPISAPAELWQESGRWDLYGKELLRFKDRHER
                     DFCLGPTHEELITDLVRRSVRSYRELPVNLYQLQTKFRDEVRPRFGLMRGREFIMKDA
                     YSFHTDVEDCRREYANMYETYKRIFGSCGLSFRPVEADTGAIGGSLSHEFQVLAASGE
                     DAIVSCDSCEYAANVEKAEVKGKLPAARDISQESPKLNKIATPGKKTVLEVAELLKLT
                     ADRFIKTMVYKTDGGDLIAALVRGDHEINELKLRTVLGCREVTLADETEVTAATGVVP
                     GYLGPIGLTLRIIADLSVHGMRGAVTGANEANFHYIDIDQERDFTPSAFADLRLAVAG
                     DACPRCESGKLQTHRGIEVGQVFYLGTKYSEKMGATYLDAEGREQSIEMGCYGIGISR
                     MVAAAVEQNHDANGIIWPMSIAPFQVLVLPINYKEEKLRNAAESLYLDLQRAGVEVML
                     DDRDERAGVKFKDADLIGIPLRVTIGAKALDKGCIELRNRRDGKTAEVAVGDAVKIIL
                     EKIAQAVQG"
     gene            11484..12239
                     /gene="pyrF"
                     /locus_tag="VE170_00925"
     CDS             11484..12239
                     /gene="pyrF"
                     /locus_tag="VE170_00925"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYJ14022.1"
                     /translation="MTSKLELVRKHGSMRERLIVALDVDDLEQATEFVRQLSGEVGMF
                     KIGKQLFTHAGPQAVRQIQELGGEIFLDLKFHDIPNTVAKAAIEATRLGVKMFNVHAS
                     GSLEMMRLTAKEVARVCRQQKLRKPIMLAVTVLTSLNQDDLERVGVERKVADQVVRLA
                     LLTQEAAMDGVVASPHEVADIRAACGRRFVIVTPGIRPSDNKRNDQQRVMTPQDAIRG
                     GVDYIVVGRPIIEAKDPVAAARAIVADMQLGRN"
     gene            complement(12314..>12609)
                     /locus_tag="VE170_00930"
     CDS             complement(12314..>12609)
                     /locus_tag="VE170_00930"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HYJ14023.1"
                     /translation="GAKAVLIKGGHLKGSAVDLYYDGKKFVALNAPRIRSKNTHGTGC
                     TLSAAIAAGLAQGQKLETAVTNAKRYITDALRHGYRIGSGHSPVHHFYRYWKP"
BASE COUNT         2806 a         3554 c         3750 g         2499 t
ORIGIN      
        1 attctgccat tgaaagggaa aattcttaac gtcgaaaagg cgcgtttcga caagatgctg
       61 tcgtcccagg agatccgcct aatgatcacc gccttgggca ccggcgtcaa agaagactac
      121 gatcaagcca aactgcgcta tcattccatc atcatcatga ccgatgccga tgtcgacggc
      181 tcgcatatcc gtacgctgct gttaacgttc ttttaccgcc agatgcccga gcttgtggaa
      241 aagggttatc tctttatcgc ccaaccgccg ctctacaaaa tcaaaaaagg caaacaagag
      301 cgctatctaa aggatgaggc gatgcttgaa gatcatctca tcgacctcgg cactgagggt
      361 gtgcgggtgc gcgcagggca aaacggcaat ggcaatggca acggtttggt cggccagccg
      421 ttgcagtcgt tcgtcaagaa aatcgcgcgc tgggaaaagc tgttgatctc cattggccgt
      481 aagcgaaaaa accgtacggt ggtggaagcg ctgttattgg cagaaggttt cggcgaggaa
      541 atgttaaagg atcgtcaagc gctgcaagat ttggagagcc gtttggcgac ttatgtcggg
      601 ttggcggcgc ctgagcaggc gccgttgtca tgcgccctcg aggaagatgc cgagcataat
      661 tgctttaagc tggtcgttac tacccgtgcc aacggcacag gctatcaaac cgtcatcgac
      721 cgcgaattct tgatcagcgc ggaattcaga gaaattaaga aattgttcgg cgatctatcc
      781 gccatcggcg tgccccccta tgccatcgaa gacggcgacg ccaaggcaca gctgaaatcc
      841 tttagcgcga tactttccta cgtcatggaa ggcggcaagc gcgggcagta tatccagcgc
      901 tataagggat tgggcgagat gaaccccgaa cagctttggg aaaccaccat gaatcccgag
      961 actcgcgtgc ttttgcaggt gcgtgtcgag gatgcggtgg aggccgatct gattttttca
     1021 acgttgatgg gcgatgaagt cgaaccccgg cgtaagttca tcgaggacaa tgccctgtcg
     1081 gtgcgaaacc tagacattta gtgtgcggtt ggcggcgctg gcagcaactt gcccggcgcc
     1141 gtagtcagag ataattcatc tagcagattg gcgagcgatt catggaagct actatcaagc
     1201 cgcaaaaggt tccggtctac atcgaagaag agatgcgcga gtcgttcatg gcctacgcca
     1261 tgagcgtgat catcagccgg gcgctgccgg atgtgcgcga cggcttgaag ccggtgcatc
     1321 ggcgcgtgct ctacgccatg tacgacgcca gcaacacttc ggataagccg tataagaaat
     1381 cagcccgcct ggtcggcgat atcatgggta agtatcatcc ccatggcgac acggcgattt
     1441 atgacaccgt ggtgcgcatg gcgcaggatt tcaacctgcg ctatccgctg gttgacggcc
     1501 agggcaattt tggttccatc gacggcgata atccggcggc catgcgttat accgaaattc
     1561 gcatgactct gttggccgag gaaatgctcg ccgacatcga aaaagacacc gtcgatttta
     1621 tcgccaatta cgacgattcg ctcaaggaac cgtcggtgct gccgtcgcgg attcctaatt
     1681 tactcatcaa tggctccgcc ggcatcgccg tcggcatggc gaccaatatt ccaccgcata
     1741 atctgagcga agtgatcgat gccttggtgg cgctggtgga caatcctgag ctgacgatca
     1801 agcagttgat gcgccatatc ccgggaccgg attttcccac cggcggattt attcatggcc
     1861 gcgatccgat tgttcgcgcc taccatgagg gcaaaggtat tgtgcaaatg cgcggcaagg
     1921 cctttaccga aacggtcaag cgcaccggca aagagcagat catcatccgg gaaatccctt
     1981 accaggtaaa taaaaagcga ctgatcgaac agatcgccga gctggtaaac gacaaaaaga
     2041 tcgaaggcat cagtgacctg cgcgatgaat cggaccgcga cggcatgcgt atcgtcatcg
     2101 agctcaagcg cgacgcggtg gctgaaatta tcatcaacca gctgcacaaa cacacccaat
     2161 tgcaagaatc cttcggtatg aacatgctcg ccatcgtcga tggcaagccc aagctgctga
     2221 cgctcaaaga ggcgctgaaa tgctttctcg atcaccgaaa ggaagtgatc acgcggcgca
     2281 ccgcctacga tctgcgcaag gccgaagagc ggttgcatat cttggacggg tttcgtatcg
     2341 cgctggacaa cctcgacgcg gtgatcgcgc tgattcgcaa gtcagccgac ggtaaagaag
     2401 ccaaagaagg cttgatgagc aatttcgcgt tgagcgagtt gcaggctcag gcgatccttg
     2461 acctgcgctt gcagcgcctc accggcttgg aacgcgacaa gatcatggcg gagcaccgcg
     2521 agaccgtcga gctgatcgcc aagctgcggg caattctcgc cgatgaaaaa gaaatctaca
     2581 agatcatcgt cggcgagctc aaggagatca agaagcagtt tggcgacgag cgccgcaccc
     2641 agatcgtcga tcattcggaa gatatttcca tcgaggacat gatcgtcgac gaagatatgg
     2701 cggtaacgat ctcccacgag ggctatatca aacgcaaccc ggtgactctc taccgggcgc
     2761 agcgccgcgg cggcaagggc aagatcggca cgacgaccaa ggaagaggac ttcgtcgagt
     2821 cgttgttcat cgcctcgatg cactcctata ttcttttctt taccaccgtc ggcaaggtct
     2881 attggatcaa ggtccatgaa ctgccccaag cgagccgtgc ggcgcgcggc aagccgatcg
     2941 tcaatctgct caatatcgag cctgacgaaa aggtttcggc atttctctcg gtacgggaat
     3001 ttcaagaagg acggttcatc attttcgcca ccaagaatgg tctgatcaag aagaccgagc
     3061 tcatggccta cgcccatccc cggccgagcg gcattcgcgc cattggcctc gaagaccacg
     3121 acgaagtcat cggtgtgcgc ctgacagacg gccagcagga gctgattctt tcaaccgccg
     3181 agggtcagtc gattcgtttc aaggaagaac aggtgcgcgc caccggccgc ggcaccttcg
     3241 gtgtcgtcgg catgcgcttg gacgaaggcg atcacgtcgt tagcatggaa attctcaccc
     3301 gcggtttcgc cgttttgacg gtttccgagg gcggctacgg caagcgcacc gagatggatg
     3361 aataccggct gcaatcgcgc ggcggcaagg gcattatcac gatgaagacc agcgaccgca
     3421 ccggccgcgt tgtcggcgtg cagcaagtca ccgaggagga ccagctcatg ctggtctcta
     3481 atatcggtaa gatcattcgc ctgcggatca aggatattcg catcatcggc cgcaacaccc
     3541 aaggcgtacg cttgatcgaa ctcgaagaag gggagcggct ggtatcgctg gcgcgggtgg
     3601 cggaaaaaga ggaagaggac gacggcggag gcgacgccta ggcacgccgc tcttagcccg
     3661 gcaacgccaa actagcttta gttgtacttg aatgttttga cgtagagctt gtcgtgagcg
     3721 gactcgcagg agctagccgc aaaaaaacca caaaagtatt gcaggcagaa tcatatcaat
     3781 cgcccatagg cggcggagcg gcgctttccg cgttccaaaa taacgactgt tttcttgtgt
     3841 gaatcgacca tggctagaga aattggcgtc atcggcgccg gcggttgggg cacggcgctg
     3901 gccaaggtgc tggccgacaa aggcgagcag gttactctgt ggtgtcatgg agccgcgagt
     3961 ttttgcgaac tgacggaaca ccggcagaat ttcacctatc tccctggcgt tgcgctgccc
     4021 gaaaaacttg ccgtaactca ggtgctaaaa aatgccgtgg ccggtaaatc gctggtgatc
     4081 tgcgccgtgc cctcccatgc ggtgcgcgaa gttttcaccg atgcggcggc gtacctcgat
     4141 ggccaaacgc atttgctgtg cggcacaaag ggattggaag agacgacctt gcataccatg
     4201 gctgagctgt tcgcggatat tttcggcacc gccagcaagg ggcgccatgg ctttctttcc
     4261 gggcctacct ttgcgagcga agttgcccag ggtttgccgg ccgcggtgac agtcgcttcc
     4321 acttcagaaa gcgtggcgcg ctcgattcaa gggattctca gcacggctag ttttcgggtt
     4381 tatacctcgc tcgatgtggt cggagtgcaa atcggcggcg tggtgaaaaa tgtcatcgcc
     4441 atcgccgcgg gcattagcga cggcctcggc ctcggccaga acgccagggc tgcgttgatc
     4501 acccgcggtt tggcagaaat catccgtttg gccgtgcgca tgggtgccga tccggcgact
     4561 ttggcgggat tgcctggttt gggcgattta gtgttgacct gtgccggtga cttgagccgc
     4621 aaccgggcag tgggcttgca gatcgccgcc ggcaagagtc tgtcggagat caccgcatcg
     4681 actcgcaccg tggctgaagg ggtgcgcaat acccgcgcgt tgcacgcttt ggctcagcgg
     4741 ttggcggtgg aaatgccgat cgtcgagcag atgtaccagg tgctctatgc agcgaagcgg
     4801 ccggacgagg cggcgcgcga tttgatgcag cgctcgctga aacccgaagc cggttaaaat
     4861 tgacttcacc gggacggtat gattgatata gtcacttggc tgtgcaggcg gcctgaaggg
     4921 gaagaagtag tcttatgagc aaaagaacct atcaaccgag caacatccgc agaaaacgca
     4981 cacacggctt tctcgcgcgc atggcgactc cgggcgggcg caatgtcatc aaacgtcgcc
     5041 gcgccaaggg acgcaaaaaa ttgactgtgt cggtgccacc taagcgggcg cgcacgtaac
     5101 tacgggggaa ggcgctgaag gataatcaag cgttcccgaa atcggcccgc ctgagaaaac
     5161 gacgcgaatt tttgcgatta tcgcgatccg gttacaagat ccattctgca aactttgtcg
     5221 tcatcagcaa gactagcgat catgccgagt cccgggtggg tatcacggtt agcggtaaag
     5281 ttggcaattc ggtgatccgc aatcggatca aacggcaagt acgggagttt ttccggcgcc
     5341 gccgcgcggc attgccgcaa gcggtggatt tcctcgtcat cgcgcgtaaa agcgcggtga
     5401 acctgccttc cagtatggtc gatgtcgaat tggccgaaag tttctcgccg ccacgcggac
     5461 gccggatggt atgaccgcat gatccgctgg ttattgatct tgccgattgg cgcatatcgc
     5521 ctgttggtct cgccctggtt gccgccggct tgccggtttt accccagttg ctcggactac
     5581 gctcaaacgg caattcaccg tcacggcgcg gtcaaaggtc tgctgtacac tctatggcgt
     5641 ttctgccgct gccacccttt tagcgccggc ggatacgatc cggtcagttg aacttaatca
     5701 atggataaac gcgcactcat cggcatcgca ttatcggtcc tggtcttggt cggttaccaa
     5761 gagtttatta cccgctatta tggcccgccg ccgacagttc cggcgccggc cgcggaaaaa
     5821 acagagtccg ctccaccggc caacactgtg tcgccctctg gcactactcc cgccgcgccc
     5881 agtgcaatgc cggcgcaagt tgcgccggcg atcgtaccgc ctagccaagc ggcgcgaaat
     5941 gtgcagatcg aaaccgataa ctacatcgcc gttttcacca gccatggcgc gcgtttgaaa
     6001 agctttcagt tcaaacatta tcgcgccagt gcggctccga atagtcctct ccaagaaatg
     6061 atcacgacgg cgaaagatgt gccactgcct ctcggcgtgc gctggcaggc gccggcgcca
     6121 ttcgatgacg ccgggttggc ttactccgtg cagggcgccg atcttaaact cacgggcgaa
     6181 gccaagggca ccttggtatt tcaaggtcag accgccgatg gtgtgacgat taccaagacc
     6241 ttgggtttta ccggcaatct ttatccgatt cagttcgatg ttacggtcca gtccgccgcg
     6301 ggcaatgcgc cagtgccgga aattttgttg accgataaag ccgatcatac cgtgccccat
     6361 cccggcgcgc cgttcgaggg ctttgtcgcc ttagtcgata acaagatcaa gcgcgaggcg
     6421 acggtggacg cgagcagtgg atatgagttt agcggcgacg tcgcgtgggc cggtttaggc
     6481 tatacctatt ttttcgttgc gttgatgccg gaaaatggcg ccgggcacaa ggtttcggtg
     6541 cgcctaaatg gcgccgcgct aatcgctacc gtgagcggac cggcggccac cgaccgctat
     6601 cgtctgttca tcggacccaa agaactcgaa ctcctaaaat cggtgggcaa ggacatcgac
     6661 cgtgccatcg accatgggta tttcggcttc atttcggtgc cgctgctcta tgtgctccat
     6721 ttctttcacc gtttcaccgg cagttatggc gtcgatatca ttatactgac cgtgctgatc
     6781 aagctaatca tgtggccgct gacgcataag agctttagct ccatgaagtc gatgcagaag
     6841 ctcgggccgc aaatggaaaa gctcaaagaa cgctatgcca acgacaagga gaaactcaac
     6901 aaggagatca tggatctgta caagcgcaac ggtgtcaatc cgttgggcgg ctgcttgccg
     6961 atggcgctgc agtttccggt tttcatcggt ctttacaacg cgctcagcac gcccatcgag
     7021 ctgcgccatg cgccgtttgt gtggattaaa gatttgtcgc gaccggactg ggaggcgctg
     7081 cagtttgccg tgcccgtctg gtggccttcg ttcttgccgt ccggcattcc gattctcacc
     7141 atcgtcatgg gcgccaccat gtttatccaa caatggatga caccgtcagc gggcgatccc
     7201 aaccagcgaa gaatgatgat gctgatgccg cttatgttta cattcatgtt cgtatcgttt
     7261 cctgccggtt tgaccgtgta ctggctcgtg aacaacgtgc tcagcatcgc gcagcagtac
     7321 tggatcaacc gcagcgacaa gtagccgagg aagggcagtt atggatttcg tcgaaaccga
     7381 aggcgataac atcgacgcag ccatcgaaaa cgcgctgaag gcgctcggcg tcgggcgcga
     7441 aaagatcacc atcgatatta tttccgaagg acgcaaagga atactcgggt ttggcgcgca
     7501 gaaagcgaag gttcgcgcct cgttgcgcaa gctcaatctc gaagccgacc cggcgccagc
     7561 caagccccag gccgcgccag tccagcaggc accggcacga gcagcggagc ccagaccccg
     7621 gccccccgag cggcgcccgg ccgaaccgcg ccaggcacaa ccatcaagat ccgagccaag
     7681 aaacagggct ccggatccca ggccgcgagc gcctgaggcg cggcctcgat ccgagccccg
     7741 cccgactcct gttcctacgc cccgtccgcc ggaggagccg caagctgccg atgcgccgcc
     7801 gccgcttaca ccagcggagg tcatggcgat ggtggtaaag gcgaagacgg ccctggttga
     7861 gattctcaat ctaatgggca tgaacgcgac tgtgacggaa aaagccggca tcgacagcga
     7921 ggaaacgatt ctcgaagtca aaagcgagaa cagcggcttg attatcggcc gcaagggcca
     7981 gacattagag gcgttgcaat acctagtcac gcggatcgcc ggcgaacggc ccggtaatga
     8041 cgcgccgcat gtcgtcatcg atatcgagag ctatcacgag cgtcgccgcc agactttgca
     8101 agacatggcg ctgcgcttgg gtgagaaggc caagcgccag cgcaagaccg tcaccatcga
     8161 cgcgctcagc gcggcggacc gcaaggtcgt tcatgcagcg ctgcaagacg atccttggtt
     8221 gaccacgcgc agtctcggcc aaggcgccta tcgacgtttg ctaatcattc ccgagggcga
     8281 tcgtaaaatt agagatgaag gtccggcacc ggcgcaagag cgcgagggtg gacggccgca
     8341 acgaagcagt cggtctaatt cgccgcctgg tgacagccgg tcgccaatgc gccaattcgg
     8401 aggtcaagaa agaccgggtg gtgacaaccc agaggacccc gaaatcgccg gggacaagga
     8461 cgactcggaa tcgaagggtg gaacgtaaga gtcctactcg cgatagggtc ggcgacaatg
     8521 gccaatcggc caaggacacg agcagagttg tcgcttcagg gtaaaatggt taagttgacc
     8581 ctcgggaatc ggttctctat agttgggcca tgattgaacg gcgcaaaacc aagcagattg
     8641 aactgggcaa agtgaaagtc ggcggtgacg cgccgatcac cgtccaatcg atgaccaaga
     8701 ccgacacccg cgatgtgcgt gccacggtgc agcagatttg gtcattggaa gcggccggtt
     8761 gcgaaatcgt tcgttgtgcg gtgccggtgc gggaagcggc ggagcaactc ggcgagatca
     8821 agaaaaaaat ccgcattcct ctggttgccg atatccattt caattacaaa ctggcgttga
     8881 tcgcgatcga gcagggcgtc gacggtctgc gcctcaatcc cggcaacatc ggtgcgcgca
     8941 agtatgtcga agaagtggtc aaggccgcgt cggagcgcaa gattcccatc cgcatcggcg
     9001 tcaatgccgg ttcgctggaa aaagatttac tgcaaaagta taacggccca accgctgcgg
     9061 gcatggtcga gagcgggctg cgccatattc atattctcga agacgtcggc tattcggaaa
     9121 tcaagatttc gctcaaagcg tcggatccgt tgatgatgat cgaagcctat cgcatgctcg
     9181 ccgaccaggt gaattatgct tttcatctcg gcgtcacgga agccggcaca ccgggcatcg
     9241 gcacgatcaa atcggcggtg gggttgggaa cgctcttggc cgaaggcatc ggcgatacca
     9301 ttcgcgtatc attggcggcc gacccggtgg aagaggtgcg ggtcggcttg gagatcttga
     9361 aagcgctcaa gttgcgcaag gacggtatga cctttgtcgc ctgtccgtcc tgcggccgcg
     9421 cggatgtcga tctcgtagcg ctggcgaagg cggtggaaga gcgcatgctg ccatatagca
     9481 atcataacat ccacgtcgcc gtgatgggct gtgaagtcaa cggcccgggc gaagcgcggg
     9541 cagcggacct cggcgtcgcc ggcggtaagg gcatcggtct gatcttcaag cgcggtgaag
     9601 tcattcgcaa agttcccgaa gcgcagattg ttgacgcgct gatggaagaa gtcgtcaagt
     9661 tcatcgccga acaacccgct gcagccgcga cggccgcacc cagctgagct tctctgtccg
     9721 gttcgagatt accaattcta aattgagtca ctatgcgttg gaataagaca ctcatcccga
     9781 cgttgaaaga agacccggcc gacgccgagg tcatcagtca caagctcctg gtgcgcgccg
     9841 gctacattcg ccagatcagc cgcggcatct acgattactt tcccttggcg ctcaaagtca
     9901 ttcgcaagat cgaaaccatt gtgcgccaag aaatggaccg cgccggcgcg caggaacttt
     9961 tgctgccgat ttccgccccc gccgagctgt ggcaggaaag cggccgttgg gatttatacg
    10021 gtaaggaact actgcgcttc aaggaccgcc acgagcgcga tttttgtctc ggtccgaccc
    10081 atgaagagtt gatcacggac ctggtccggc gttcggtgcg ctcttaccgc gagctgccgg
    10141 tcaatttgta ccagctgcaa actaagtttc gcgatgaagt gcggccgcgc ttcggcttga
    10201 tgcgcggccg tgagttcatc atgaaggacg cctacagttt tcacaccgat gtcgaggatt
    10261 gccggcgcga atacgccaat atgtacgaga cctacaagcg catcttcggc agctgcggtc
    10321 tgagctttcg accggtggaa gcggacaccg gcgccatcgg cggcagcctg tcccatgagt
    10381 ttcaagtgct ggccgcgtcc ggcgaagacg ccatcgttag ctgtgatagt tgcgaatacg
    10441 ccgccaacgt cgaaaaggcc gaagtcaagg gcaagctgcc ggcggcgcgg gacatttccc
    10501 aggaatcccc caagctgaac aagatcgcca caccgggtaa aaagaccgtg ctcgaagtcg
    10561 ccgagttgct caaattaact gccgatcgat tcatcaagac gatggtttac aaaaccgatg
    10621 gcggcgattt gatcgcggcg ctggtgcgcg gcgatcatga aatcaacgaa ttgaaactgc
    10681 gcacggtgct gggttgccgc gaagtaaccc tcgccgacga aaccgaagtg acggcggcga
    10741 caggcgttgt gcccggctat ttggggccga tagggctgac gttgcgcatc atcgcggatc
    10801 tgtcggtgca cggcatgcgc ggcgcggtca ccggcgccaa cgaagccaat ttccattaca
    10861 tcgatatcga tcaggagcgc gactttacgc cgagcgcctt cgccgacttg cgcctggcag
    10921 tggcgggcga tgcctgtccg cgctgtgagt cgggcaaact gcagacccat cgcggcatcg
    10981 aagtcggcca agttttttat ctcggcacca agtacagcga gaagatgggt gcgacctatc
    11041 tcgacgccga gggccgcgag cagtccatcg agatgggctg ttatggtatc ggcattagcc
    11101 gcatggttgc cgccgcggtc gaacagaacc acgacgccaa cggcatcatc tggccaatgt
    11161 ccatcgcgcc cttccaggtg ctggtcttgc cgatcaacta caaagaagaa aaactgcgca
    11221 atgccgccga gtcactctat ctggatttgc agcgcgccgg cgttgaagtc atgcttgacg
    11281 accgcgacga gcgcgccggc gtcaagttta aagacgccga cctgatcggc atcccgctgc
    11341 gcgtcaccat cggcgccaag gccctggaca aaggctgtat cgagctgcgt aaccggcgcg
    11401 acggcaagac cgccgaagtg gcggttggcg acgcggtgaa aatcatcctt gaaaaaatcg
    11461 cccaggcggt gcagggataa accatgacct caaagctcga gctggtacgt aaacatggtt
    11521 ccatgcgcga gcgattgatc gttgccctcg atgtcgacga cttggaacaa gccaccgagt
    11581 ttgtccgcca gctctccggc gaagtcggca tgttcaagat cggcaagcag cttttcaccc
    11641 atgcggggcc gcaggcggtg cggcagattc aagagctcgg cggcgagatc tttctcgacc
    11701 tcaagtttca cgacattccc aacacagtag cgaaagcggc catcgaagct acccggctcg
    11761 gcgtgaaaat gttcaatgtc cacgcctcgg gcagcttgga gatgatgcgc ctgaccgcca
    11821 aggaagtagc cagggtgtgc cgccagcaaa agctgcgcaa gccgatcatg ctggcggtca
    11881 ccgtgctgac gagcctaaac caagacgatc tcgagcgcgt cggcgtcgaa cgcaaggtcg
    11941 ccgaccaggt agtgcgtttg gctctgctga cccaagaagc cgccatggac ggtgtcgtcg
    12001 cctcgcccca tgaagtcgcg gatattcgcg cagcatgcgg ccggcgcttc gtcatcgtca
    12061 ccccggggat tcgtccgtca gataacaagc gcaacgatca acagcgcgtc atgacgccgc
    12121 aggatgccat tcgcggcggc gtcgattaca tcgtcgtcgg ccggccgatt atcgaagcca
    12181 aagatccggt tgccgccgcc cgcgccattg tcgccgacat gcaactggga cgtaactagc
    12241 tcatagctgc gccggcgcga acattgccca tggcaaattc cacatggcaa attttgcgcc
    12301 ggcgcgaatt cgcctagggt ttccaatatc gatagaaatg atgcaccggg ctgtggcctg
    12361 agccgatgcg gtagccgtgg cgcagcgcat cggtgatgta gcgtttggca ttggtcaccg
    12421 ccgtttcgag cttctggccc tgggccaggc ccgccgcaat ggcggcggag agagtgcagc
    12481 cggtgccgtg ggtgtttttg ctacggatgc gtggcgcgtt cagtgccacg aattttttac
    12541 cgtcgtaata aagatccacg gccgatcctt tgagatggcc gcctttgatg agcacggctt
    12601 ttgcgccca
//
LOCUS       DASQKS010000038         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5479988, whole genome shotgun sequence.
ACCESSION   DASQKS010000038 DASQKS010000000
VERSION     DASQKS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5479988"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VE170_00935"
     CDS             complement(<1..584)
                     /locus_tag="VE170_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ14024.1"
                     /translation="MFDFLFPNHPFGGHTLRLVAQAQQGGADVFDIARAMKNVEAGDK
                     DAWEREWLALAEQTAARAHSALAAGHQGTARQNYLHASNYYRMSDVLLTIADESKRGE
                     RFRQSQAMFRAAAELGPAKIEVISVRCGDEQYDGYFCHPVNPKPGKWPAVLFLGGADA
                     YAEEIYFGGKQMLERGWAMLLVDTPGRGSSIYLKG"
     gene            1012..2084
                     /locus_tag="VE170_00940"
                     /pseudo
     CDS             1012..2084
                     /locus_tag="VE170_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
     gene            2388..2801
                     /locus_tag="VE170_00945"
     CDS             2388..2801
                     /locus_tag="VE170_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYJ14025.1"
                     /translation="MDSILPLLDAPKLWGVEDAQVTLVGWGSTEGVVQEAIQKLAAEE
                     GIAANLLQIKWLVPLHGEAIISTLAKNKKTIVVENNYSGQFARYLRSETGIVADGHIR
                     KYDGEPFMPHHIVDGVKAILAGGTKQYVPVHEIMV"
     gene            2927..>3155
                     /locus_tag="VE170_00950"
     CDS             2927..>3155
                     /locus_tag="VE170_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14026.1"
                     /translation="MTLSTVELEAKSSVPPPANLPLTVKDFKGKAEPDWCPGCGDFGV
                     LVALKQALAELGIRPHQAMVISGIGCSSNLPG"
BASE COUNT          676 a          877 c          967 g          635 t
ORIGIN      
        1 cctttcagat atatggagga gccgcgtccc ggcgtgtcga ccaagagcat cgcccagccg
       61 cgctcgagca tctgcttacc gccgaaatag atctcttccg cgtaggcgtc ggcgccgccg
      121 agaaacagca ccgccggcca tttgcccggt ttggggttca ccggatggca gaagtaaccg
      181 tcgtactgtt catcaccgca gcgcactgaa atcacttcga tcttggccgg gccgagctct
      241 gcggcggcgc gaaacattgc ctgggattgg cgaaagcgtt cgccgcgttt gctttcatcg
      301 gcgatggtca ggagcacgtc ggacattcga taataattgc tggcatgtaa atagttctgc
      361 cgcgccgtgc cttgatggcc ggcggccaat gcgctgtggg cgcgggctgc agtctgctca
      421 gccaacgcca accattcgcg ctcccaggcg tctttgtcgc cggcttcgac gttcttcatc
      481 gccctggcga tatcgaagac atcggcgccg ccttgctggg cttgggcgac caggcgcagc
      541 gtgtgaccgc cgaatggatg attgggaaaa agaaaatcga acatgctgcc tccggaatgg
      601 ccgctgaatg aacgctctct actgtttgcg gaaaaagccc agaaataatc cgaatcgaag
      661 caaaagtaca aggcctattc agcgcaggac aaaagattcc cttgacagcc gcaatgaccg
      721 ttggtagcgt ttcgatcaca aacagatttc ctcaacattg atagactctc gcgattcacg
      781 gcaacggtgc aaggagcgcg gtgtgatatt tagaggggaa ttgagtggcg gaaagccggt
      841 tgcttttgcg acatgtcgcg ggagcaaacc ggcttttttt tcgccgagtg cgcgcttgcc
      901 gggcgacagt tttcatggct aagggcatgg ttcgacttac cgccgactag ggtttcggaa
      961 gggactgcaa ataaggagcg gcgtggcacg acgatacagg aggaatggca aatgacgaga
     1021 gtcgatcttt ctatcgcaat cggcggcgca gccggccagg gcatcgccac gcccggcgac
     1081 atcctggcgc gcatcttcgt gcgccgcggg ttgcatctaa acgcctacaa tgcgtatcag
     1141 tcgatcgtgc gtggcggtca tatttttctc accttgcgca cgagcgacgc gccggtgctg
     1201 agcttcggtg acaagctcga tgttttaatc gctctcaatc aggatacgat gaatcgccac
     1261 ctccgcttga tgacgccggg ctcttcagtg ctttataacc aggacaagat caaacctgac
     1321 ggcgctcgac cgggcgtgca gctttgcggt ttttcggtca aagaactggc gccggcggtg
     1381 cgcggcgact tggtgcagaa taccatcgcg ttggcggcgg tgttgcagct gatcggcgtc
     1441 gaattcaaaa ttctcgaaga gatactcaac gtgcagttca agagaaaagg cgaggcggtg
     1501 atcgccgaaa acgtcggcgt ggcgcgcgcc ggttacgact acgcggcgaa gcagttcaaa
     1561 gcgctgcccg tctcactacc ggataccggc aggaaactgg cgttcttcga aggcaaccaa
     1621 gcgttggcca tgggcggtgc cgcggcggga gtgcgctttt actgcgccta tccgatgagc
     1681 ccgtcgtccg gcgtgcttca ttggatggcg cgccatgggc gccaattgga cattatggtg
     1741 cgccaagtgg aggacgagat cggcgtcatc aatatggcca tcggcgcggc ccacgccggc
     1801 tgccgcgcca tgtgcgcgac ttcgggtggc ggattcgcgt tgatgaccga gggactgggt
     1861 gccgcgcgat gatggaaata cccgtcgtct gtatcaacgt ccagcgtgcc ggcccggcca
     1921 ccggagtgcc gaccaaaacc gaacaaggcg atctatggca ggcgatgggc gcgggacagg
     1981 gtgactaccc gcgcatcatc gtggcgccga cgacgatccc ggattgtttt catacggtgc
     2041 cgcaactgtt caatctggtc gacaaatttc aatgtcccgg catcatcctt tcagatttat
     2101 tgctgtcgga gggccgatcg agcatcgacc cggctgagtt ggacttcaac gtcgccatcg
     2161 aacgcggcga ggtgatcggc ttaaatggcg ccacgccgcc gctcaacggc ggctataagc
     2221 gctacacgat caatgacagc gggatctcgc cgcgggcgct gcccggcacg cagggattcg
     2281 aacatgtggt cgccaccgac gatcacgatg aagacggcgt gctgatcagc gacgaatata
     2341 ccaaccctca taagcgccaa gcgatgcacg aaaaacgcat gcgcaagatg gattcaatct
     2401 tgccgctgct cgacgcgcca aagctctggg gcgtcgagga cgcgcaggtc accctggtcg
     2461 ggtggggctc gaccgaagga gtcgtccaag aggcgatcca gaaactagcc gccgaggaag
     2521 gcatcgccgc caatttgctg cagattaaat ggctcgtgcc gttacatggc gaagcgatca
     2581 tttcaactct cgcgaaaaac aagaaaacca tcgtcgtcga aaacaattat agcggtcagt
     2641 tcgcgcgtta tttacggtct gaaaccggca ttgtcgccga cgggcacatt cgcaaatacg
     2701 acggcgagcc gttcatgccg catcatatcg tcgatggcgt gaaagcgatc ctggccggcg
     2761 gcacgaagca atatgtgccg gtgcatgaaa ttatggttta gcaatcgttt cttgtgggtc
     2821 gggtgcggag ggggattcac cgtacggtca cacagagcgg gaagcagcgg gcgcaaagtt
     2881 gtgcgctcct gcaactcata acgcgaaaca agttaaagga gcgctgatga cattatctac
     2941 ggtcgaactt gaagcaaaat cttcggtgcc gccgccggcg aatctgccgt tgacggtcaa
     3001 ggattttaaa ggcaaggccg aaccggattg gtgccccggc tgcggtgact ttggcgtgct
     3061 cgtcgcattg aaacaggcgc tcgcggaact gggtattcgc ccgcatcagg cgatggtgat
     3121 cagcggcatc ggttgttctt ccaatcttcc cggct
//
LOCUS       DASQKS010000039         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1700848, whole genome shotgun sequence.
ACCESSION   DASQKS010000039 DASQKS010000000
VERSION     DASQKS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700848"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VE170_00955"
     CDS             <1..735
                     /locus_tag="VE170_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14027.1"
                     /translation="DPQLLSEFYQKHFALEEVKIFPSGSRMVIDGLFNLAFLKRREQV
                     SAVIGTHRSDGAELERQPGIHHYGFMVDDLPSAVAKLPAWLDRATSPQASDGVGGVEG
                     ARPAEMRFIDPWGNNVDLSSRGFLGREEKKLPGVRLVVIQVPDVEAAREFYQGQFDLV
                     LVGHDADGSVRLSDGTLTIKLTREQSQAKSGIQYFGIEVTRLAPVKHSLGTAGVAFAE
                     DSAGQIRLRDPEGNAVVVSEQSWAKG"
     gene            772..1074
                     /locus_tag="VE170_00960"
     CDS             772..1074
                     /locus_tag="VE170_00960"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYJ14028.1"
                     /translation="MIVVSNRLQVAKGHEADFEKRFEGRARLVEHMAGFIRLEILRPI
                     KSEYYIVLTHWQDEASFRAWTDSAEFKEAHNKRPPAEIFAGPNVFEMHEVIQQAGN"
     gene            1222..1611
                     /locus_tag="VE170_00965"
     CDS             1222..1611
                     /locus_tag="VE170_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14029.1"
                     /translation="MPKLRHLAIRTEDTGKLAKFYMDVFEMQILHKERDEGGVIYLTD
                     GYFNLAILPNKEQNSPNGLYHFGFEVESGEKIVERLKKINPNKLPKQRPNDRPYAETR
                     CSDIDGNFFDISEHGFLDYELVGDRKK"
     gene            1727..2722
                     /locus_tag="VE170_00970"
     CDS             1727..2722
                     /locus_tag="VE170_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14030.1"
                     /translation="MRRGLLSLLLALTFVPAELSAQVRSNFASSITSESMASVWVARQ
                     LGLFKKYGLETQYILMPRSPLAVAALLANEIDVAVIGPGHLVNAGASGADVIGIANFV
                     QKLDYRVNVRPDIKKKEDLRGKKIAISGPGATSHLVTLLALQHMAIDPNQAKITFLTI
                     PGTEINRRLALESGSIDATSLNGSVGDTYANKGYPIIYNFKGSGVTMPQTMLVTTRRI
                     AAAKPQVIEGYSKGLVEAIAYMLDPGNKENVTRIMATNLRLSNTSEADEAYQAVVNAY
                     ERLPLPTVEGMKRLHGLLLTINPKLPEVKVETLIDDSFIHRLEASGFVQNVSKKR"
     gene            3021..>3724
                     /locus_tag="VE170_00975"
     CDS             3021..>3724
                     /locus_tag="VE170_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ14031.1"
                     /translation="MKEEQVSVVVVGGGSAAFEAAVAAKQSGAGQVVMLEKAPEPEFG
                     GNARFSHTGFRFAYSGAAEIRGFMPNMSAAEFDRLHLPGYTPADFTADLMRVTRGRIN
                     KKLADVLVNESNVAVHWMDQLGMQWEPAGHVEIDGKFYFEPGIVIHPTGGIGGGISQL
                     NQWRDIATRMGVTIRYESRVRELLGNDRHVEGVRVSDPREEYEIRAGATILCAGGFQA
                     NPEMRARYLGANADLM"
BASE COUNT          873 a          979 c         1090 g          782 t
ORIGIN      
        1 gatccgcagt tgctcagcga gttttaccag aagcatttcg ctctggaaga ggtcaagata
       61 tttcccagcg gctcgcgcat ggtgatcgac ggcttgttta atcttgcttt cttaaagcgg
      121 cgggaacaag tctcggctgt aatcggtacc caccgcagcg acggagcgga attggaacgg
      181 cagccgggca ttcatcacta tggcttcatg gtcgacgatc tgccgagcgc ggtggcaaag
      241 ctgccggcct ggctcgatcg cgccaccagc ccgcaggcgt ccgacggcgt gggcggcgtg
      301 gaaggggcgc ggccagcgga aatgcgcttc atcgatccct ggggcaacaa cgtcgatctg
      361 tcgtcacgcg gttttctcgg ccgagaagag aaaaagcttc cgggagtgcg cctggtggtg
      421 atccaggtgc cggacgtgga agcggcacgc gagttctatc agggacaatt cgatctggta
      481 ctcgtcggcc atgacgccga tggttcggtt cgattgagcg acggcacgct taccatcaag
      541 ttgacccgcg aacagagcca ggcgaagagt ggtattcaat attttggcat cgaggtgacg
      601 cgcctcgctc ctgtgaagca ctcacttggt actgccggcg tcgcgtttgc cgaagattcc
      661 gccggacaga ttagacttag agatcccgaa ggcaatgcag tggtcgtgtc ggaacagagt
      721 tgggcaaagg gatagccttt tgccgatgct ttcaacgaaa ggaagtggag cgtgatcgtc
      781 gtatccaatc gccttcaagt agccaagggt catgaagcgg attttgaaaa gcgcttcgag
      841 ggccgcgccc ggctggtgga gcacatggcg ggatttatcc gcttggaaat tctgcggccg
      901 ataaaaagcg aatactacat tgtcctgact cactggcaag acgaagcttc gtttcgcgcc
      961 tggaccgaca gcgcggagtt taaagaagcc cataacaagc gcccaccggc ggaaatcttt
     1021 gccggcccga atgtgtttga gatgcacgaa gtgattcagc aagcgggaaa ttagcggcaa
     1081 aaagatcgat ttcatcggcg ataattttgc ccgcgagcgt tttggccggg gaacgttgcg
     1141 ccaacgagca aaacgccttc acttgacatc gccctgcctg gggcattact ggtggagtta
     1201 atcgattcta aggaggtact catgcctaag ctacgccatt tggcgattcg aacagaagat
     1261 accgggaagt tggcgaaatt ttacatggat gtttttgaga tgcagatact gcacaaagag
     1321 cgagacgaag gcggcgtgat ttatttgacg gacggttatt tcaatctcgc tattctgccc
     1381 aacaaggagc agaactcgcc caacgggctc tatcactttg gcttcgaggt cgagagcggc
     1441 gagaagatcg tcgagcgatt gaaaaagatc aatcccaata aattgcccaa acagcgcccg
     1501 aacgaccgtc cctacgcgga aacccgttgc tccgatatcg acggcaattt tttcgatatc
     1561 tccgagcatg gttttctcga ttacgaattg gtcggcgacc ggaagaagta aaaagctagt
     1621 tcaaaccgtt gcaccgctgc attccgttca aaccattgga acttgccaaa acggtttgaa
     1681 acgtttgcgc caccttcatg gatcgaagtt accggaggac tgagtaatgc gccgtggctt
     1741 gttgtctctg ctcctagcac tcacgtttgt cccagccgag ctgtcggccc aagttcgttc
     1801 taacttcgcc tccagcatta ccagcgagag catggcctcc gtctgggtcg ccagacaatt
     1861 gggcttgttc aaaaaatatg gccttgagac ccagtatatt ctcatgccgc gctcgccgct
     1921 ggcggtggcg gcgttgctgg ccaacgaaat cgatgtggcg gtgatcgggc cgggccattt
     1981 ggttaacgcg ggtgcgagcg gcgccgatgt gatcggtatt gcgaacttcg ttcagaaact
     2041 ggactaccgg gttaacgtgc gtcccgacat caagaagaaa gaagatctgc gcggcaagaa
     2101 gatcgctatc agcggccccg gcgcgacctc gcacctggtc acgctgttag cattgcagca
     2161 tatggcgatc gatccgaatc aagcgaagat tacgtttctg acgattcccg gcaccgagat
     2221 caaccgtcgt cttgcgttgg aaagcggtag tatcgacgcc acctcgctga acggttcggt
     2281 gggggacacg tacgcgaaca aaggctatcc gattatctat aatttcaaag gttcaggcgt
     2341 gaccatgccc caaaccatgc tggtcacgac ccgccggatt gcggcagcca agccacaggt
     2401 tatcgaaggg tactcaaaag gactcgtcga ggcgatcgct tacatgctcg atccgggtaa
     2461 caaggaaaac gtgacgcgca tcatggctac gaacttacgg ctgtccaata cgagcgaggc
     2521 cgacgaagcc tatcaggcgg tggtcaatgc ttacgagcgg ctgcccctgc cgacggtgga
     2581 aggcatgaag cgactgcatg gcttgctcct gacgattaat ccaaaacttc ccgaggtcaa
     2641 agtcgaaact ttgatcgacg acagcttcat tcataggctt gaagcttccg gttttgtgca
     2701 gaacgtcagt aaaaaacgtt aaggagtgat cagccagagg atcaccgaaa cctgcctgta
     2761 gatcactcga cagtatcttt gcaaagcaaa caaaacactc agaacaagat ccgcagctta
     2821 gggaagcgat tcattgcggc ggctgtggtg ccatatcctg aacttgcgca aaacgcagga
     2881 aactcagcga tcacccaatc gagccttcgg tacgggagaa attggcggca aagtatgcaa
     2941 agcctacaat aaacaaactt cgctatgaac aacggcggtc aacccgccgt ggtgatttgt
     3001 gcgggaggcg ggagcaaacg atgaaagaag aacaggtttc agtcgtagtt gtcggcggcg
     3061 ggagcgccgc ctttgaagcg gcggtggcag ccaagcaaag cggtgcaggt caagtcgtta
     3121 tgttggaaaa agcgcccgag ccggagttcg gtggcaacgc gcgtttctcg cacaccggat
     3181 ttcgcttcgc ctattccggg gcagcggaaa ttcgcggctt tatgcccaat atgagcgctg
     3241 ccgagtttga ccgcttgcac ttgcctggct atacgccggc ggatttcacc gccgatctca
     3301 tgcgtgtgac gcgcggccgg atcaacaaga aactcgctga tgtcctggtt aacgaatcca
     3361 acgtcgccgt ccattggatg gatcaactcg gcatgcagtg ggagcctgcc ggccacgtgg
     3421 agatcgacgg caagttttat tttgagcctg ggatcgtcat ccatcccacc ggcggcattg
     3481 gcggcggcat aagccagctc aatcagtggc gcgatatcgc cacacgcatg ggggtcacga
     3541 ttcgctacga gtcgcgggtg cgcgagctgc ttggcaatga ccggcatgtc gaaggcgtgc
     3601 gtgtatccga tcctagagag gaatatgaga tccgcgccgg cgcgacgatt ctctgcgccg
     3661 gcgggttcca ggccaatccg gaaatgcgcg cgcgctatct cggcgccaac gccgatttga
     3721 tgaa
//
LOCUS       DASQKS010000040         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3380484, whole genome shotgun sequence.
ACCESSION   DASQKS010000040 DASQKS010000000
VERSION     DASQKS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3380484"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VE170_00980"
     CDS             complement(<1..482)
                     /locus_tag="VE170_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14032.1"
                     /translation="MPTKIQHAAIISENFVREARFFEAAFGMKRSKPGSEEEQKAIRT
                     NYAVSVSDGYVGMTIIGRKPGYVPGLHHFGLDVDDVDEAIKRIRKDYPEVAVLKRPSN
                     RPFATFGAHDPEGNYFDLTQEGMSNRRDVYTEQQREQPRRMHHIKLRVMNAPAMAAFY
                     R"
     gene            complement(862..1938)
                     /locus_tag="VE170_00985"
     CDS             complement(862..1938)
                     /locus_tag="VE170_00985"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14033.1"
                     /translation="MANLRRTFSLTRSRFMGEGTGLCGRALIGAQLLLVALAVPFAVA
                     AAELRTVMVLSYPDRPAKLPLWLAQDAGLFTKYGLNVEIKTPNSGEAIADGARNHAAD
                     IYVATANWMVSAIGDGADLVFFANTGYSVLKLLSQPSITRPEQLRGKRVGTGEAGSSQ
                     DRITRETLRRLGLDADKDVTLVPFGSRSVLRVNALLKGEIDATTSNEDNIFDLERRGE
                     IDKVRVLADNDSLKLYIGAGVDFAATRKLLAGSRDAVKRFTQALGEATALARRDRGAA
                     NGIYTGYLKVSDPGLLEFMYRTYVQHAIPEKPYPKIDNVALGITEFAAKPGLKGKSAE
                     TLTDSSLVRELDQEGFFARLNPGR"
     gene            complement(2406..3842)
                     /locus_tag="VE170_00990"
     CDS             complement(2406..3842)
                     /locus_tag="VE170_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14034.1"
                     /translation="MAMTGALFRCALLVLSLLFSACSMVGSSRPIVIQGGTLIDGTGR
                     GAIADSVIVIRDGRFAAVGKRGDVAMPQDAEVIDARGKTLLPGLIDGHCHYRDWMGEL
                     YLAYGVVTCPNISNNPVEWITAQREGVKNGSVRGPRVWASANIIDGPPPQGTGTLRRQ
                     RTSIIVDSEEEARKAVRGLVAQGVDGIKLFERLKPHVAKAAADEAHKLGRPVFGHSLD
                     IFTAAANGFQSVEHSWSVVYTSIQDPKKKNDLDIGRMLGKIDTAEVHAHMEPAMFDKI
                     IKVMIDKNVHWSTTWATWFRPLSSYAESMKKRELTLLRNPQLKYLPPYILKDTESFFA
                     KYEKMAPAKRQEVVAGFKMLQDFVRRFAGAGGKIHSGSDPNHVVPGYALHAELEMLVE
                     AGLTPVQAIRTAALNVAEAWGKEKDFGSVESGKVADLVMVGGDPLKNISDTQNVEMVF
                     SAGTKVDTSFHADYKNPMPRPIEDRPEP"
BASE COUNT          777 a         1235 c         1084 g          864 t
ORIGIN      
        1 cgataaaatg ccgccatcgc cggcgcgttc atgacgcgca gcttaatatg gtgcatgcgc
       61 cgcggctgct cgcgctgctg ttcggtataa acgtcgcgac ggttagacat gccttcttga
      121 gtcaggtcga agtagtttcc ttcgggatca tgagcgccga aggtggcaaa gggccggttc
      181 gacggccgct tgagcacggc gacctcggga tagtctttcc tgattctctt gatcgcttcg
      241 tcgacatcgt cgacgtccaa gccgaagtga tgcaaacccg gcacgtagcc gggcttgcgg
      301 ccgatgatgg tcatgccgac ataaccgtcg ctcacgctca cggcgtagtt ggttctgatc
      361 gccttctgct cttcctccga accgggcttg gagcgcttca tgccgaaggc cgcttcgaaa
      421 aaccgcgctt cgcgcacgaa gttctcactg ataatcgccg cgtgttggat cttggttggc
      481 atcgggcctc ctttggtcgg cttgctcaat gttcaagttt gcagttcgct gtggacgatt
      541 tcatcaatcg cggctcaatc gtccgctcgt cgatttccag tttaacacgg tcttaatatt
      601 cgactcccga ctctcgatgc catcaagcgc aggtttccca aggcctgtct aacttgttcg
      661 gccgggtctc ccgcggcgac cagctcgccc ctcctgtcca ccgggccgat tcggggaaaa
      721 acgtacacgg tctccagcgc attgcggaaa acttgaatgt cgcccgccgc cgcctggcca
      781 tgcgcgagtg tcgacgcggc tgcgagcatt aacagcaaag ccgcgccaag ccaagcataa
      841 agcataaccg ttcgattcac ctcaacgccc cggattgagc cgggcaaaga aaccttcctg
      901 gtcgagttcg cgcaccaagc tcgagtcggt cagtgtttca gccgattttc cctttagtcc
      961 cggcttggcc gcgaactctg tgatgcctag cgcgacgtta tcgattttcg gataaggctt
     1021 ttccgggatc gcgtgctgca cataagtccg gtacatgaat tccagcaacc ccggatcgct
     1081 cactttcaaa taaccggtgt aaataccatt cgccgcccca cggtcgcgcc gcgccaaggc
     1141 ggtcgcttcg ccgagcgctt gggtgaatcg cttcaccgcg tcccgcgagc cggcgagtaa
     1201 cttgcgcgtc gctgcgaagt cgacacccgc gccgatgtag agtttcagac tgtcgttgtc
     1261 ggccaagacg cgaaccttgt cgatctcgcc gcgccgttcc aaatcgaaga tattgtcttc
     1321 attgctggtg gtcgcgtcga tctcgccttt gagcagcgcg ttgacccgca gcacgctgcg
     1381 gctgccgaac ggcaccaggg taacgtcttt gtcggcgtcc aagccaagcc ggcgcaaagt
     1441 ctcgcgcgtg atgcggtctt gcgagctgcc cgcttcgccg gtgccgaccc gcttgccgcg
     1501 caattgctcc ggccgagtga tcgatggctg cgacaacagc ttgagcacgc tgtaaccggt
     1561 attggcgaag aaaaccaaat ccgcgccgtc gccgatggcc gacaccatcc agttggcggt
     1621 ggcgacataa atatccgccg catgattgcg cgcgccgtcg gcgatcgcct cgcccgagtt
     1681 cggcgttttg atttcgacgt tcaatccata cttcgtgaaa agcccggcgt cctgggccag
     1741 ccacagcggc agcttcgccg gccggtcggg ataggaaagc accatgacag ttcgcaactc
     1801 cgcggcagcc acggcaaaag gaacagcaag cgcaacaagc agcaattgtg cgccgatcaa
     1861 ggctcgcccg cataatccgg ttccctcgcc cataaaacgg gagcgggtga ggctgaaggt
     1921 tcttcgaaga ttagccatcc tcaccttctt tttttccttc tctggaggga gagaacaaaa
     1981 gcgaacaaac cgttttgaat ccgccgttgg tcgtcataaa gctactccca ggtctaccgt
     2041 gaagttgcac ccaccacaaa gtgtccatga gcttgcgctc tccttagagc atgaaaatgg
     2101 tcggcattcc caaaacatgc tcccctcctc gattcgtcat tctcgcgcac gcgggaatcc
     2161 aggtttgttc tgcgcccaat tagcctggat acccgctgtc acgggtatga cggaaccccg
     2221 gaggctcttc gttgtgccta acatcccttc acaggtagtt tcgaagaaga gcacgaagac
     2281 ggcggaggtt tcggagaatg aacattctta acttcgtgat cttcgtgtct tcgtggtgca
     2341 catcccaact gaacgttgca cattattcac tcaagaacct gaaaaatccc attcaatcac
     2401 tcatctcatg gttccggccg gtcttcgatg ggccgcggca ttggattttt ataatcggcg
     2461 tgaaacgaag tgtcgacctt cgttccggcg ctgaatacca tttcgacatt ttgcgtgtcc
     2521 gagatattct tcagcggatc gccgccgacc atgaccagat ccgccacctt gccgctctcg
     2581 acgctgccaa agtctttttc cttgccccac gcctcggcaa cgttgagcgc cgcggtgcga
     2641 atcgcttgca ccggcgtcaa gccggcttcg acgagcatct ccagctcggc gtgcagcgcg
     2701 taaccgggca cgacgtgatt gggatcggag cctgaatgaa tcttgccgcc ggcgccggca
     2761 aaccggcgca cgaaatcttg cagcattttg aagcccgcca ccacttcctg gcgctttgcc
     2821 ggcgccatct tctcgtactt ggcgaagaag ctttcggtgt ctttgagaat atacggcggc
     2881 agatatttca gctgcgggtt tctcagcaag gttaactcgc gtttcttcat gctctccgcg
     2941 taactcgaca gcggccgaaa ccaagtcgcc caggtggtgc tccaatgaac gtttttgtcg
     3001 atcatcacct tgatgatctt gtcgaacatc gccggttcca tgtgagcgtg cacttcggcg
     3061 gtgtcgattt tgccgagcat gcggccgata tcgagatcgt ttttcttctt cggatcttga
     3121 atcgacgtgt agaccaccga ccaggaatgc tccaccgact gaaacccatt ggccgccgcg
     3181 gtgaaaatat cgagagagtg gccgaacacc ggccggccga gtttgtgcgc ctcgtccgcc
     3241 gccgccttgg cgacatgcgg tttgagccgc tcgaatagtt tgatgccatc gacgccctga
     3301 gcgaccaggc cgcgcaccgc tttgcgcgcc tcttcctcgc tgtcgacgat aatgctggtg
     3361 cgctggcgcc ggagcgtgcc ggtgccctgc ggcggcgggc cgtcgatgat gttcgccgac
     3421 gcccacaccc gcggcccgcg cacgctgccg ttcttcacgc cctcgcgctg cgccgtgatc
     3481 cattccactg ggttattgga aatattcggg caggtgacca ctccgtaggc gagataaagt
     3541 tcgcccatcc aatcgcggta gtggcaatgg ccgtcgatca agcccggcag cagcgtcttg
     3601 cccctggcgt cgatcacttc ggcatcctgc ggcatcgcca cgtcgccgcg cttgccgacc
     3661 gcggcaaacc tgccatcgcg gatcacaatc accgaatctg caatcgcccc gcggcccgtg
     3721 ccatcgatca gcgtgccgcc ttggatgacg ataggccggc tcgagccgac catggaacag
     3781 gccgagaaca gcaggcttag caccagcaat gcgcagcgaa ataaagcgcc agtcatagcc
     3841 ataagtgttg gctcctccaa attgacgtga agtggaattc accgctgagg cggacagttc
     3901 gcatggttcg acgagctcac caccctcgat acataagaaa acaacgaccc aatatctgct
//
LOCUS       DASQKS010000041         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1701214, whole genome shotgun sequence.
ACCESSION   DASQKS010000041 DASQKS010000000
VERSION     DASQKS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701214"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(835..1116)
                     /locus_tag="VE170_00995"
     CDS             complement(835..1116)
                     /locus_tag="VE170_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14035.1"
                     /translation="MYVHFSGERYHDMGGRSTLVEIGVAKTLAEAEAIRDKEDEFENI
                     VSEKLDLAFTELRARLKERGFYKEIDDQTLLNGMGTWLLAKRNYLFDIN"
     gene            1105..1368
                     /locus_tag="VE170_01000"
     CDS             1105..1368
                     /locus_tag="VE170_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14036.1"
                     /translation="MDIHIELSQNDVDYLLHHLPLGTALRQKLGYSYLTPGFPVERPV
                     EFSNEIDCSEGDALALLSIAREYCPEAVQKIQDGLRLSGITSY"
     gene            1695..1970
                     /locus_tag="VE170_01005"
     CDS             1695..1970
                     /locus_tag="VE170_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14037.1"
                     /translation="MSGWISQNSDTFERCLDSLFPGLNPRMEQVDKFWRLWHTFATTM
                     IECNKNPLWIANQMGTSLEMLFSTYAVYFQKRGWSQKWSQGVIAGGK"
     gene            complement(1999..2073)
                     /locus_tag="VE170_01010"
     tRNA            complement(1999..2073)
                     /locus_tag="VE170_01010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2038..2040),aa:Arg,seq:ccg)
     gene            2412..3275
                     /gene="ispE"
                     /locus_tag="VE170_01015"
     CDS             2412..3275
                     /gene="ispE"
                     /locus_tag="VE170_01015"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYJ14038.1"
                     /translation="MKIRAPAKINLNLRVIGRRADGYHLLDTIMVPVSLYDEIDIQQR
                     AITRRAAPLAPIEIRCNNPQVPLGRDNIAYRAAELLMQKHKIKRRVCVRIQKKIPIGA
                     GLGGGSTDAAAVLVGLNRMWKLRLSVRQLERLALQLGADVPFFIRAKPARARGIGEKL
                     SLLPKLRRRWLVLAYPGFPVATAWVYRNLPVKLTKVGVNTSIATPLERLDTLEKLLVN
                     DLERVAIRRYPEIGRVKTMLSLAGAVAVLMSGSGSSVFGVFRSKQWAEKAYRRMQREE
                     GVQAFLVHVLN"
     gene            3344..3631
                     /gene="spoVG"
                     /locus_tag="VE170_01020"
     CDS             3344..3631
                     /gene="spoVG"
                     /locus_tag="VE170_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HYJ14039.1"
                     /translation="MQVTEVRVFPVEEDKLRAYVTITLDHCFVIRDLKIIHGATGLFV
                     SMPSKKRKDGTYKDIAHPINNETRRMIEEKIIAEYQKVLADSEAPTAIHDD"
     gene            3680..3753
                     /locus_tag="VE170_01025"
     tRNA            3680..3753
                     /locus_tag="VE170_01025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3712..3714,aa:Gln,seq:ttg)
     gene            3906..>4825
                     /locus_tag="VE170_01030"
     CDS             3906..>4825
                     /locus_tag="VE170_01030"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYJ14040.1"
                     /translation="MGRTADLKIFAGNSNPPLATEISEYLQVPLGNATVGRFSDGESK
                     IEIKENVRGADVFVVQSTSAPGNDNLMELLLMLDAFKRASAQRITAVMPYYGYARQDR
                     KVVPRVPISAKLVADLITRSGASRMLTMDLHSGQIQGFFDIPVDNVYATPVLLDYLRK
                     QVDHTEVTIVSPDAGGVERARAIATRLDASLAIIDKRRVGPNIVAEMNIIGEVKDRIA
                     ILVDDMVDTAGTLTMAAEALKKAGARKIIGCCSHAVLSGPAIKRLEECPLEQLIVTNT
                     IPLGPQAQACGKIKILSVAHLIGEAIRRTH"
BASE COUNT         1120 a         1245 c         1309 g         1151 t
ORIGIN      
        1 gtcatcaaag agcacaaact cgccgcttac ctagcacaga tcgaatgaga gccgtcttct
       61 agaggggtcg taacacagat ataacatact ggtgctaatt cgaggagata gtgaaacact
      121 tctccgattg gatcatcgtc gtgttttatg ggcgctgtcg cagtaggttt atttgtgtga
      181 tgacccgatg atcgaccgat attaagctat tcgcaaccgg acgtagagga cggaaatcag
      241 tgggaactgg gagaagtcac gccggagcgc gcgttcaaat aactacattc ttgatcaatc
      301 caatttctcc ctgattcaaa gccgcacgct tccgcgtctt tttcatccgc gaaaacctga
      361 cgagtggcta tttttattag gatgtggccg ccctctgggt gaacgtctac cacaggcgta
      421 aataattctc cgttatacga gtcccggatg atgtgaacgt caatgtaatg attcctgtga
      481 ataaaatgag taagcatctg gtactcctct cagtgttatg aaaagtatgc cgtaattggt
      541 cgtgatttca gttcggtatt agactggtgg gcgctgtctc gtattggaca aatctccgcg
      601 acagtcagtg aatcagctta aacttgccgg cgcgttcaat tcgtattctg tccgtcctcg
      661 ctttacgacg cagcagatcg ccggtgagac gatcgagtat catcctgcga aattacccac
      721 gatgggtaat ttgccctccc ccaagcgcaa aaggcttagg cgatcccaaa tttctaaccg
      781 ttggaaattt gctatccgcg gcgccgagcc caaagacccg gacgccggcc gcgatcagtt
      841 gatgtcgaac agatagttcc tctttgcaag aagccaagtt cccatcccat tgaggagtgt
      901 ctgatcatca atctctttgt aaaatcctct ctcttttaac cgggcccgaa gttcagtgaa
      961 tgccaaatca agtttttcgc tcacaatgtt ctcgaactcg tcttctttgt cgcgtatggc
     1021 ttccgcttcc gccagggttt ttgccacgcc gatttcaaca agcgtgctcc ttcctcccat
     1081 atcgtggtat ctttcaccac taaaatggac atacatattg aactatctca gaacgatgtt
     1141 gactacctgc tccatcattt accgctcggt acggctctca ggcaaaaact tggctactcg
     1201 tatttgactc ccgggttccc cgttgaacga cctgttgaat tctccaatga aattgattgc
     1261 agcgaaggcg atgcactcgc gttattgtct atcgctcgtg agtactgccc tgaagcggtt
     1321 caaaaaatcc aagacggcct gagactgtcc ggcataacat cttattgata ggccccacca
     1381 gaacaaactc gcgggcgccc gagcccgtag atccggcgcc gcagtttacg cccgccgctg
     1441 attctgggat ttgcacaggc ccttacaacg gtgaaaatcc tttaggcggt atagacggaa
     1501 gagcaacccg cggcagaccg atcttgatct ccgcgagttg cccattattt accaaagcgt
     1561 gtcgaccaag tattgagcca tctttctcat tttctcaggt ggatagccgg cgagccgcgc
     1621 gccgagaagc atgccgagtt tgaggccgac ttcttggcgt agatacttca gatagtggaa
     1681 tcatccacga gatcttgagt ggctggatct cccaaaacag tgatactttc gagcgctgcc
     1741 tcgattcgct gttccctggg cttaatccac ggatggagca ggtcgacaaa ttctggcgcc
     1801 tctggcacac gttcgcgaca acgatgatcg aatgcaataa aaatccgctc tggatagcga
     1861 accaaatggg cacaagcttg gaaatgctgt tcagtacgta cgcggtttac tttcagaagc
     1921 gcggatggtc acaaaaatgg tcacaagggg ttattgcagg tggcaagtaa tttgatggat
     1981 tcgcttttaa tatcagggtg gtgcgcccga caggaattga acctgtactt agggtttcgg
     2041 agaccctcgt gatatccatt tcactacggg cgcgtggcag attttctatc attgggtcgg
     2101 cgatcagtca acgtactttg atctgtatcg cggcagcgat tctaccgggt tgctcatgta
     2161 gcagagggtt gtaaaagcgc ggctagccca gggcggagtt tgttgtgccg tcatgccgat
     2221 caacgatcca ccaatacgaa cttttcgata cggcggcgtt aatcgctact cttgagttcc
     2281 tgcaagttgg caaggtaatc tcgctcgcgc atcggccgct tgccacgcgg ctcaaagaag
     2341 tccgcttaag tctggtgtcg tggcttgtca aactttcgca atcatccagc ggctatgtta
     2401 gctagtggcg tgtgaagatt agagcgccgg caaaaatcaa tttgaacttg cgggtgatcg
     2461 gcagacgcgc cgatggctat cacctgctcg acacaatcat ggtgccggta tcgctctatg
     2521 atgaaattga cattcagcag cgggcgatca cgcggcgcgc cgccccgctt gcgccgatag
     2581 aaattcgctg caacaacccg caggtccctt tgggtcggga caacatcgcc tatcgcgccg
     2641 cagagttatt gatgcaaaaa cacaaaatta agcggcgtgt ttgcgttcgc attcaaaaaa
     2701 aaattcccat cggtgcggga ctgggtggcg gtagtaccga cgccgccgca gtattggtcg
     2761 gtcttaatcg catgtggaaa ttgcgtttgt cggtgcgcca attggagcgg ctggcgctgc
     2821 aacttggcgc cgacgtgcca ttctttattc gcgccaagcc ggcacgggcg cgtggaatcg
     2881 gcgaaaaact tagcttgttg ccgaagctgc gccgccgctg gctggtgcta gcctacccgg
     2941 gattcccagt ggcgactgca tgggtatacc gaaatctccc tgtaaagttg acaaaagttg
     3001 gcgtaaatac tagcattgcg acgccgctgg agcgcctgga tacgctcgag aaattactcg
     3061 tcaacgatct cgaacgggtc gccataagga gatatcctga aatcggtcga gtgaaaacga
     3121 tgctgtctct ggcaggcgcc gtggctgttc tgatgtcggg aagcggctcc tcggtatttg
     3181 gcgttttccg gtcgaaacaa tgggctgaga aagcctatcg tcggatgcag cgtgaggagg
     3241 gggtccaagc gtttctggta catgtcttga attaaggtta gtcaaaagcc cataaaaaga
     3301 actctaccgg cgcaacatct ggctcgtcca gaagggggtc cctatgcaag ttaccgaggt
     3361 tagggtcttt cctgtcgagg aagataagct cagggcttat gtcactatca ccctcgatca
     3421 ctgtttcgtg attcgggact taaaaatcat acatggcgcc acgggtctgt ttgtctcgat
     3481 gcccagcaag aagcgtaagg acggaaccta taaagatatc gcgcatccga tcaacaatga
     3541 gacgcgccgg atgatcgagg aaaagataat cgcagaatat caaaaagtgc tcgccgacag
     3601 cgaggcaccg acagcgatcc atgacgattg atcggatgtt ctgcgcgacc ggatgagctt
     3661 cccagtggga gttcacggat gggcggtcgc caagcggtaa ggccccggcc tttggagccg
     3721 gtatcgaagg ttcgaatcct tcccgcccag ccagtttctt ctgcaagatt gtccggctgg
     3781 cctttgcgat attgatttag tagcttgagg ggcgccgttc acggagggac ggcgctgctg
     3841 tttttggagg cagaggtgac tggggttacg aactgaagac ttgcactcga gggtagtggt
     3901 aaacgatggg tcgcacagcc gatttgaaaa tattcgccgg gaactccaat ccgccgctgg
     3961 ccaccgagat cagcgagtat ctccaagtac cgctgggtaa cgccacggtt gggaggttta
     4021 gcgacggcga gagcaagatc gaaatcaaag agaacgtccg cggcgcggat gtgtttgttg
     4081 tgcagtccac ctcggcgccc ggcaacgaca acttgatgga gctcttgctg atgctcgatg
     4141 ccttcaagcg cgcatctgca caacgcatca cagcggtcat gccttactat ggctatgccc
     4201 gccaggaccg taaagttgtg cctcgggtgc caatcagcgc caagttagtc gcggatttga
     4261 ttacccgatc cggcgcgtcg cgcatgttga ccatggatct gcactcggga cagatccaag
     4321 gattcttcga tattcccgtc gataatgttt atgccacccc ggtgctcttg gattatctgc
     4381 gcaagcaagt cgatcatacc gaagttacca tcgtctcgcc ggacgccggc ggcgtggagc
     4441 gggcgcgggc gatagccacg cgtctggatg catcgttggc gattatcgat aaacgccggg
     4501 tcggtccgaa tatcgtcgcc gaaatgaata ttattggcga agtcaaagac cggatagcca
     4561 tattggtcga cgatatggtc gataccgccg gcaccttgac catggctgct gaagcgttga
     4621 aaaaagccgg cgcgaggaaa attatcggtt gttgctcgca cgcggtattg tcaggcccgg
     4681 cgatcaaacg cctcgaggag tgcccgctcg aacaattgat tgtcaccaac acgattccgc
     4741 ttgggccgca ggcccaggca tgcggcaaga tcaaaatatt gtcggtcgct cacttgatcg
     4801 gcgaggcgat ccgccgcacc catga
//
LOCUS       DASQKS010000042        10166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2123276, whole genome shotgun sequence.
ACCESSION   DASQKS010000042 DASQKS010000000
VERSION     DASQKS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123276"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VE170_01035"
     CDS             <1..822
                     /locus_tag="VE170_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYJ14041.1"
                     /translation="FTVSYVRNNLLFEALWLLPLGVFVGTVGTLIGAGGGFILVPVLL
                     LLYPNEAPATITTISLIVVFFNAVSGSLAYARTKKIAYKTGVIFSLAAIPGSILGAFA
                     TAAVKRQQFDLLFGGILSLLSMLLLIRPARKSSVGQSQTSLAENPPLGPAKLILGTLT
                     STGLGFLSSFLGIGAGFIYVPVFVYLLDFPILTATATSLFILSLMSLTGAATHILAGM
                     LDHGMNLALVLSPGVICGAQIGARLSRRIHADWILRGLGLALGLVGMRFVIAAWR"
     gene            complement(875..2074)
                     /locus_tag="VE170_01040"
     CDS             complement(875..2074)
                     /locus_tag="VE170_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14042.1"
                     /translation="MKLSTFKLLVPIVAVTLQSNSSSTVIPPYLDHMHVPVALIGTLI
                     SLGPVFALMARLPIGMLYNRGRARGLVSAAIMAMGVTNYCYSFAVSSWSFAVVHCLNG
                     FAYSAVTTLYMAFYVDSLAPDENRNHAMGYYVGCLAVGYSTGNFLGGLIADHWGYSWT
                     FQLGALLSFVAVALLWFLHGPTGDAASQGKRSEPAKLSVRESCKALLEPELATVVLVA
                     LFLNLLHQMGGVFISLYGLGVGMSLTQIGLIRAAYAGCNAVTRPISGHVVNKVGHKGL
                     SYFGLPLQSLILMLVPLFTGFGTILVVYVASGFMRAIVVVANAVGLVQDVPESRVRRG
                     LASGVYNAAGDVGNILGPSIGGFIAHATGIASVFVVGSLGSTALFLLGVVLVRRLKQD
                     QTPTPDS"
     gene            2294..3178
                     /locus_tag="VE170_01045"
     CDS             2294..3178
                     /locus_tag="VE170_01045"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HYJ14043.1"
                     /translation="MDLIRSWMFVPGHRQKMIDKALGLQADAIMLDIEDGVAPNEKDS
                     ARQNIGASLGRAKAPGSPARYVRTNSIGHPWMDADLAAVVRPGLEGLVCPKVDTVDDV
                     RKVDALLDDMEPKNQLARGSVRLLIAIESPKGLFNAPAIAAASSRIVGIIFGTEDFGR
                     EIGLPAIREGEARDLIYARSAIVVAAAAAHVQAVDGVWVDLNDGAGLHSFAKQSRQLG
                     FSAMSCIHPSQIEAINTIFSPTVAEIDYCQKVLQAFAEANARGDGSIAFGGQLIDRPI
                     VERARRTVDMAKSLGMIQ"
     gene            3211..4143
                     /locus_tag="VE170_01050"
     CDS             3211..4143
                     /locus_tag="VE170_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ14044.1"
                     /translation="MKLKIPLTLLALGCLTMGAAAPVSMTPIKIADNVYMLQHSQGSG
                     NSTVIFTNEGVVVFDSHIDNSDQTLAFIRKTTDKKIKYLITSHSAGDHASGSWHFRED
                     KPVWIATKHQIHDLQMQELKEFNLRRDSNDPRYAVYKGKEQGLPDIGIDGPMTLYFGG
                     LTFQIIPEGRGHSTGDLTVYIPQKRIMLMGDLLDTEIHPGQGESGEIFYSNVKGWIQI
                     LDSIMARSLPVETYIPGHGPAHIGRGVKDLEEQKRYFVVMRDEVAKMIAAGKTLEQIE
                     KEFKVPQEFAHYTRPERLRSFYKLFYHQLMETGY"
     gene            4156..5202
                     /locus_tag="VE170_01055"
     CDS             4156..5202
                     /locus_tag="VE170_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14045.1"
                     /translation="MKPIAGALLIALSIPGSTWAQGKMSTEQLAAYNKPDREKMLYAG
                     AKAEAKVTWYTSLAGESYKKLAAAYEARYPGVSVESYRATRQEMSARILAESQAQRYI
                     ADTIETTIPLLKLLKDNNWLVPYYFSAMAKYPDPVKEKAPRGLVFWAIDRESHIGLAY
                     NKNLIPAKSVPRNYEGLLRPELKDKISFAGSDTGVTVTGALLKFKGEEFIRKLKTLNP
                     SVHNVSGRALLDMVISGEVGVSPSTFRNHVEVSLKAGAPIEWIPMDVVPANSGSTAVL
                     AKAPHPHAALLLADFILGPGGQKVLEEYEYGNPTKDYGFKRWYPEQGLTAEQIDALEG
                     KWRNTMRDMTRRSF"
     gene            5441..6910
                     /locus_tag="VE170_01060"
     CDS             5441..6910
                     /locus_tag="VE170_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ14046.1"
                     /translation="MNTGSYVNGKWYRPAANEIGRNVNPADTDDVIGEFPYATKTDVG
                     KAIDAAQAAFPAWRKTPGPERGRVIWRAAEIARRRADEIARTLTREEGKILKEAKGEV
                     MKGISLLEYYAGEGFRLHGKTLPSEARDTFTYTMRQPLGVVGLIAPWNFPWAIPVWKS
                     APALVAGNTVIFKPSELTPATAALLTEIYQEAGLLPGVFNMLVGNGPEVGEAMVNAPE
                     LRAISFTGSNEVGAALYVKVAQRGAKVTCEMGGKNAVIVMPDADLDKAALAIHGGAFG
                     STGQRCTATSRVIAHPDIKNLLLERLLEGAKKIKIGAGLDETIDMGPLVDNQQWTSVL
                     NYIRIGQQEGGKLVLGGKRPEKLSKGYFVEPTIFDNVTPAMRIFKEEIFGPVLSIATA
                     KNLDEALRFANGVEYGLTTAIFTENIDTIMKFVDEVETGMVHVNEPTIGGEAQLPFGG
                     IKSTGVGDREMAEEGLNFFTELKTVFINYSGKAERSMTR"
     gene            complement(6996..7364)
                     /locus_tag="VE170_01065"
     CDS             complement(6996..7364)
                     /locus_tag="VE170_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14047.1"
                     /translation="MAKIRHIAYRAVDVDAMAKFFVDAMGMQMIQKRKNNAIDLSDGS
                     VNITVLPLAAGTGGQAGIDHIGFSTDNDEEQFKSLEAAGAEKAGAVNLGTAYYEDKFR
                     GPEGIIVDVGHWQGAAPVDK"
     gene            complement(7444..7947)
                     /locus_tag="VE170_01070"
     CDS             complement(7444..7947)
                     /locus_tag="VE170_01070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYJ14048.1"
                     /translation="MDADAKKTALRMIPYGIYVLTAESKDGKVAAATVNWVTQTAFAP
                     PLVVVGVKADSGAHAIIKESKVFALNMLGKDQTKQAFTFFKSLERDGNTIGGEAFRKG
                     SLGAPILENAPAFVECQLVDTIEKGDHSIFVGEVKDAGVAVAPAGRPDDAILAMRDLG
                     EKVFYGG"
     gene            complement(8067..8627)
                     /locus_tag="VE170_01075"
     CDS             complement(8067..8627)
                     /locus_tag="VE170_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14049.1"
                     /translation="MDSIVFRRAVATDYSAIVQLNAANFIANLSEAERRDGFLSALFT
                     MEQTAAMAEQLGTTVAVADGQVAGFLCAFRNDFDHGSPVVAKMIESYDRLSFQDRLLN
                     TYRSYAYGPVCIGRAYRRKGLLRGLFEEQKRQLAGRFEVGVALIARTNQHSLDAHIAG
                     LGMTDVGSFEVEGNVFATVAFRVRPN"
     gene            complement(8636..9154)
                     /locus_tag="VE170_01080"
     CDS             complement(8636..9154)
                     /locus_tag="VE170_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14050.1"
                     /translation="MQYDPHRTGSPPKPIEPTGFFTGIQIRPIITGAVVDFIATFALV
                     AFYYRFYVAQGSGVTEGFEEEMAKYWLSADGLLASLLLGSLGTLIGGFYAAYKAGTLE
                     MKHGALVGLCSIIVGLLMQPGGSENQAPEWFLALSLAAAMPAGALGGFFAELFSGVTG
                     KKLSTTRLSTDR"
     gene            9295..9756
                     /locus_tag="VE170_01085"
     CDS             9295..9756
                     /locus_tag="VE170_01085"
                     /inference="COORDINATES: protein motif:HMM:NF021192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HYJ14051.1"
                     /translation="MFHRYDYESEYYPSLSRLPLDLRRKLDISGIKLSLKDWLAISLE
                     ERMVLCHLPCDYDEERQAFATYLDFLANKYLGTPAEKTAPMNIRSWSESAVPGAVAKR
                     SAATGQAVTPIEWRRWQSHHRYALYKTATSNNQPEAFERVLRQLRQLATHY"
     gene            9917..>10166
                     /locus_tag="VE170_01090"
     CDS             9917..>10166
                     /locus_tag="VE170_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HYJ14052.1"
                     /translation="MSLIVFIAMVATAALVGIQFGPGPWYLALQKPSWTPPNWIFGPV
                     WTALYIAIAVAGWAVWRSKAVSITKPILLWLLQLILNGL"
BASE COUNT         2326 a         3033 c         2730 g         2077 t
ORIGIN      
        1 ttcactgtct catacgtgcg gaataatttg ttattcgagg cgctttggct gctgcccctc
       61 ggcgtgtttg tcggcaccgt tggcactctg attggcgcgg gcggcggctt tatcttggta
      121 cccgtccttt tgttgcttta ccccaatgaa gcgccggcga ccatcacgac gatttcgctg
      181 atcgtggttt tcttcaatgc cgtttctgga tcgttggctt acgcacggac caaaaaaatc
      241 gcctataaaa ccggcgtgat tttttcgctt gcggcgatcc caggctcgat ccttggcgcc
      301 tttgccactg ccgccgtcaa gcgtcaacaa ttcgatcttt tattcggtgg tattttgagt
      361 cttctctcaa tgctcttact gattcggccg gcacgaaaga gcagcgttgg acaatcccag
      421 acatcgctcg cggagaatcc tcctttaggc cctgctaaac tcatcctggg aaccttaaca
      481 agcaccggct taggcttttt gtcgagcttt ttgggaatcg gcgccggatt catttacgtt
      541 cccgtctttg tctatcttct cgattttccc atcctcaccg ccaccgccac ctcgcttttt
      601 attctttcat tgatgtcttt aactggagcc gcgacacaca tattagcggg aatgctcgat
      661 cacggaatga atctggcttt ggtgctcagt cccggagtca tttgcggcgc acagattggc
      721 gcacgcttgt ctcgtcgaat acatgccgac tggatactcc gtgggctcgg cttggcgctg
      781 ggcttggtgg gaatgcgttt tgtgattgct gcctggagat agccgggtcg ggattcgcag
      841 catcaacgac cttcacaaac gctgccaggt ggacttaact atctggcgtc ggcgtttggt
      901 cctgtttcag acgacggacc aaaaccacgc cgaggagaaa cagcgccgtc gaccccaatg
      961 aaccgacgac aaacacgcta gctatcccag tggcgtgagc gatgaagcct ccgatcgaag
     1021 gaccaaggat gttacccacg tcgccggcgg cgttgtacac ccccgaggca agaccgcggc
     1081 ggacccttga ctcaggcacg tcttggacca agccgacggc gttggcgacg acgacgatgg
     1141 cgcgcatgaa tccggacgcg acatagacaa ctaaaatagt gccaaagccg gtgaacagcg
     1201 gcaccagcat caagatcaaa gactgcagcg gcaagccgaa ataggacagc cctttgtgcc
     1261 ccaccttatt caccacatgg ccgctgatcg gccgggttac ggcattgcaa ccggcgtagg
     1321 cggcgcgaat gaggccgatc tgggtcaagc tcatacccac gcccaagcca tagagcgaga
     1381 tgaacacgcc gcccatctga tgcagcaggt tcaagaacag cgcgaccaag accacggtcg
     1441 ccaattccgg ttcgagcaaa gccttgcaag attcccgcac actgagtttt gccggttcgc
     1501 tgcgtttacc ttgggacgcg gcatcgccag tcgggccgtg caaaaaccac aacagagcga
     1561 cagcgacgaa ggacagcagc gcgcccaact gaaaggtcca ggaataaccc caatggtcgg
     1621 cgatcagtcc gcccaggaaa tttcccgtgg agtagcccac cgccaggcag ccgacgtagt
     1681 aacccatggc atgattgcgg ttttcatcgg gcgcaagcga gtcgacgtag aaggccatgt
     1741 aaagcgtggt caccgcgctg tacgcgaaac cgttgagaca atgcaccacc gcgaaggacc
     1801 aactactcac ggcaaagcta taacagtaat tagtcactcc catcgccatg atcgcggcgg
     1861 acactagccc gcgggcgcgc ccgcggttgt agagcatgcc gatgggcaag cgcgccatca
     1921 gcgcgaagac cggcccaagc gatatcaaag tgccgatcaa cgccaccggc acatgcatgt
     1981 ggtctagata aggcggaatc acggtcgacg acgagttgct ctggagagtc actgcgacga
     2041 tgggcaccag gagcttgaac gtgctgagtt tcatatcgca tcagttattc gctaaaagtt
     2101 ttccggcttc aaccgaaccg ccggaaagat ttctcccggg ggtcgaaatg acgcctgacg
     2161 atggtccgcc ggctgtcatc tcgaacgcag tgagagatct cgtctgcaaa ttaccaaccc
     2221 gtccatacac cagtccagtc attagacatt cgccgcccct tcaggtaaaa attcccgcca
     2281 aggagatttt cccatggatt taatccgctc atggatgttc gtgccgggac accggcaaaa
     2341 gatgatcgac aaagcgctcg gcttgcaggc cgacgcgatc atgctggata tcgaagacgg
     2401 cgtcgcgccc aatgaaaagg actccgcgcg ccagaatatc ggcgcgtctt tgggccgcgc
     2461 caaagcgccc ggctccccgg cccgttacgt gcgcaccaat tccatcggcc acccgtggat
     2521 ggacgccgac ctggcggcag tcgtccgccc cggcttggaa ggtttggtct gcccgaaagt
     2581 cgacaccgtc gacgatgtac ggaaagtcga cgccttgctt gacgacatgg aaccgaagaa
     2641 tcaattggcc aggggtagcg tccgcctgct gatcgccatc gaaagcccca aaggactgtt
     2701 caatgctccg gccatcgccg ccgcgtcatc gcgcatcgtc gggattattt ttggcacgga
     2761 agacttcggc cgcgagatcg gactccccgc gattcgcgag ggtgaagcgc gggatttgat
     2821 ctacgcccgt tccgcgatcg tcgtcgccgc cgccgcggcc catgtgcaag cggtcgacgg
     2881 ggtatgggtc gatctcaacg acggcgcagg attgcacagc ttcgccaaac aatcgcgcca
     2941 gctcggcttc tccgccatgt cttgcattca tccctcgcaa atcgaagcga tcaataccat
     3001 cttcagtcca acggtggcgg aaattgacta ctgccaaaag gtcctgcaag cgtttgccga
     3061 agccaacgcc cgcggcgatg gctccatcgc cttcggtggc caactcatcg accgtcccat
     3121 cgtcgaacgc gcccggcgca ccgtcgacat ggccaagtct ttgggcatga tccaataatc
     3181 cgactgcacg acgtcaaagg atatgagccg atgaaattga agattccatt aacattgctt
     3241 gccctcggct gcctgaccat gggcgccgcc gcgccagtga gcatgactcc gatcaagatc
     3301 gctgacaatg tttatatgtt gcagcattcc caaggttcgg gaaattccac cgtgattttc
     3361 accaacgagg gcgtcgtcgt cttcgattct cacatcgaca actccgacca gaccctcgcc
     3421 ttcatccgca aaaccaccga taagaaaatc aagtatctga tcacttccca tagcgccggc
     3481 gatcacgcct cgggttcgtg gcactttcgc gaagacaagc cggtctggat cgccactaag
     3541 caccagatcc acgatttgca gatgcaggaa ctgaaagagt tcaacctgcg gcgcgactcc
     3601 aacgacccgc gctacgccgt ctacaagggc aaagagcagg gcttgccgga tattggcata
     3661 gacgggccga tgacgctcta tttcggcggc ctgacatttc aaatcattcc cgaagggcgc
     3721 ggccacagca ccggagattt gacggtttat attccgcaga agcggatcat gctcatgggc
     3781 gatcttctcg acacggaaat tcatcccggc caaggcgagt cgggcgaaat tttttattcc
     3841 aacgtgaaag gttggatcca aatcctcgat agcatcatgg cgcgcagcct gcccgtcgaa
     3901 acttacattc ccggacatgg gccggcacac atcggcagag gcgtgaaaga tttggaagag
     3961 caaaaacgct acttcgtcgt catgcgtgac gaagtcgcga aaatgatcgc cgccggcaaa
     4021 actttggaac agatcgaaaa agaattcaaa gtgccccagg aattcgccca ctacacccgg
     4081 ccggagcggc tgcgctcgtt ttacaaactt ttctaccatc aactcatgga aaccggttat
     4141 taaaggagat caccaatgaa acctatcgcc ggagcgcttc tcatcgctct atccattcca
     4201 ggttcgacct gggcgcaggg caaaatgtcc accgagcagc tggccgccta caacaaaccc
     4261 gaccgcgaaa agatgcttta cgccggcgcc aaggccgaag ccaaagtcac ttggtatacc
     4321 tcgctggcag gggagtcata taaaaagctc gccgcggcat atgaggcgag atatcccggg
     4381 gtttctgttg aatcctaccg cgccacccgc caggaaatga gcgcgcgcat cttggccgag
     4441 tcgcaggcac agcgttacat cgccgatacc atagagacga ccatcccgct gttgaaattg
     4501 ttgaaagaca acaactggct cgtgccttat tacttttcgg cgatggcgaa gtatcccgac
     4561 cctgtcaaag aaaaggcgcc caggggtttg gtcttctggg ccatcgaccg tgagtcccac
     4621 atcggtctcg cctacaacaa aaatttgatt cctgcgaaat cggtgccgcg caactatgaa
     4681 gggcttttgc gccccgagct gaaagacaaa atctcctttg ccggcagcga caccggcgtc
     4741 accgtgaccg gcgccttgct caaattcaaa ggcgaagagt ttatccgcaa actcaaaacc
     4801 ctgaatcctt cggtgcataa cgtttcaggc cgcgccttgc tcgacatggt gatatccggc
     4861 gaagtcggcg tatcaccctc gacgtttcgc aaccacgtcg aagtctcgct caaagccggc
     4921 gccccgatcg aatggattcc catggacgtg gtgccggcca attccggctc caccgcggtg
     4981 ttagccaagg cacctcatcc ccatgccgcg ttgctcctcg ccgatttcat tcttggaccg
     5041 ggcggtcaga aggtgctcga agaatatgaa tacggcaatc cgacgaaaga ctacggcttc
     5101 aaacgctggt accccgagca gggcttgacc gccgagcaga tcgatgcgct cgaaggcaaa
     5161 tggcgcaata ccatgcgcga tatgacgaga aggtcttttt aagttttctc acgactgcgc
     5221 ggcacttgcg tgacagagga tcaatcggcc tcaccatccg tcctgccggc aaacgcggaa
     5281 agcaggttta ttacggcacg gtgtcgagaa gcttaaattc tcgttttcac gctattgacg
     5341 actcatgctg tcagcgaagc atcttccggc agttgagaaa aaaggcagct aaaactatcc
     5401 cgcacggcat gtccgaattg tccttaaaac cgaggtcgcc atgaacaccg gctcctacgt
     5461 caacggcaag tggtatcgac ccgctgccaa tgagatcggt cgcaacgtca atcccgccga
     5521 caccgacgat gtgatcgggg aattccccta cgccacaaag acggacgtgg gcaaagccat
     5581 cgatgcggca caggctgcgt ttcccgcttg gcgcaagact cccgggccgg aacggggccg
     5641 ggtcatctgg cgcgccgccg agatcgcccg gcgacgcgcc gacgagatcg cccgcacgct
     5701 cacccgcgag gaaggcaaga tcctcaagga agctaaaggc gaagtgatga aaggcatctc
     5761 gctgttggag tattacgccg gcgagggctt tcgcctgcac ggcaaaacct tgccgtccga
     5821 agctcgcgat acattcactt acaccatgcg ccagcccctc ggcgtcgttg gattgatcgc
     5881 gccgtggaat ttcccctggg ccattcctgt ttggaaaagc gcgccggcgt tggtcgccgg
     5941 caacacggta atcttcaaac cctcggagct aacccccgcg acggccgcat tgctgactga
     6001 aatttatcag gaagccggct tgcttcccgg cgttttcaac atgctcgtcg gcaacggtcc
     6061 cgaagtcggc gaagctatgg tcaacgcccc cgagctgcgc gccatctcct tcaccggatc
     6121 caacgaagtc ggtgccgcac tttacgtcaa ggtggcgcag cgaggcgcca aagtcacctg
     6181 cgagatgggc ggcaaaaacg ccgtcatcgt catgcccgac gctgacttgg acaaagcggc
     6241 attggcgatt cacggcggcg cgttcggctc caccggacaa cgctgcaccg cgacatcgcg
     6301 ggttatcgcc catcctgaca tcaagaatct cttgcttgaa cgtttgctgg agggcgccaa
     6361 gaaaataaaa atcggcgccg gcctggacga aaccatcgac atgggaccgc tggtcgataa
     6421 ccaacagtgg acgtcggtgt tgaactacat caggattggc cagcaagaag gcggcaagct
     6481 cgttctcggc ggcaagcggc ccgagaagtt gtcgaaaggc tacttcgtcg aaccgacgat
     6541 cttcgacaac gtcacgccgg ccatgcgcat ttttaaagaa gaaatcttcg gcccggtgct
     6601 gtccatcgcc acagcgaaaa acttggatga ggccctgcgc tttgccaatg gcgttgagta
     6661 cggcttaacg actgcgatct ttacggagaa tatcgacacg atcatgaagt ttgtagacga
     6721 agtcgagacc ggcatggtcc atgtcaacga gccgaccatc ggcggagaag cgcagttgcc
     6781 ctttggcggc attaaatcga cgggggtcgg tgatcgagag atggcggaag aaggtttgaa
     6841 tttttttacc gaactgaaaa ccgtcttcat caactattcc ggcaaagccg aacgatcgat
     6901 gacgagatag cccctggcgg cgcgcagttc aacgttcaac gggtcaacgt cagaggcagg
     6961 gccttgacct tttaaacctt gaacgtctga acgatttatt tatccaccgg cgccgcgccc
     7021 tgccagtgac cgacgtcgac aatgatgcct tctgggccgc gaaacttgtc ttcgtaataa
     7081 gccgtgccga gattcaccgc accggctttt tccgcgccgg cggcttccag cgacttaaac
     7141 tgctcctcat cgttatcggt ggaaaagccg atatgatcga tgccagcctg accgcccgtg
     7201 ccagcggcga gcggcagtac cgtgatattg accgagccat cgcttagatc gatggcgtta
     7261 ttcttacgct tctggatcat ctgcatgccc atcgcgtcga cgaaaaattt cgccatggcg
     7321 tccacatcca cggcccgata agcgatatgc cggatcttag ccattggtta tctccttctg
     7381 ggtgtcaaac cttaaacttt tgaacccttg aacctttgta ccgcgcgcgg cacagcggcg
     7441 cgtttatccg ccgtaaaaaa ccttctcgcc caaatcgcgc atcgccaaga ttgcgtcgtc
     7501 cggtcttccg gcgggtgcaa cggcaacgcc ggcatccttc acttcgccga caaagatcga
     7561 gtgatcgccc ttctcgatgg tgtcgaccaa ctggcattcg acgaatgccg gcgcattttc
     7621 caagatcggc gcgccgagag accccttgcg aaaggcttcg ccgccgatgg tgttgccgtc
     7681 gcgctcgagc gacttgaaga aagtaaaggc ctgctttgtt tgatctttgc ctagcatgtt
     7741 gagggcaaac accttggatt ctttgataat cgcgtgggcg ccggaatctg ctttcacacc
     7801 gacgacaacc agcggcggcg cgaacgccgt ctgggttacc caattgacgg ttgcagcggc
     7861 cactttgcca tccttgcttt ccgccgtgag aacgtaaatc ccatagggaa tcatccggag
     7921 ggcggtcttc tttgcatcag catccatgag taaaaattcc tttcaaaaat gatttagcgg
     7981 ttccgcggtc gatccgtgaa atcgagccga ttctatggga gccaaacgaa ttgcgcaagc
     8041 cgcggttcga tcgaaccccg cccatctcag tttggcctta cgcgaaacgc cactgtggca
     8101 aacacattgc cctcgacctc aaagctgccg acgtcggtca tgccgagccc cgcgatatga
     8161 gcatcgagcg agtgctggtt agtccgcgcg atcaaagcca cgccaacctc gaaccgtccc
     8221 gccaattgtc ttttttgctc ctcgaacaag ccgcgtaaca agcccttgcg ccggtaagcg
     8281 cggcctatac acacgggacc gtaggcgtaa gaccggtagg tattgagcag tctatcttgg
     8341 aagctcaagc gatcgtagga ttcgatcatc ttagccacca ccggcgaacc atgatcgaaa
     8401 tcattgcgaa acgcgcagag aaaaccagcg acttgaccat cggcgaccgc cacggtagtg
     8461 cccagttgtt cagccatggc cgccgtctgc tccatcgtga acagcgcaga aagaaatccg
     8521 tcacggcgct ctgcctccga aagattggcg ataaagttgg cggcgtttaa ctgaacgata
     8581 gccgagtagt cagtggcgac cgctcgccgg aaaacaatgg agtccactgc gccccctacc
     8641 gatccgtcga taatcgagtg gtcgaaagct ttttgccggt cacaccggag aacagctctg
     8701 caaagaagcc gccaagcgca cccgccggca tcgcggccgc aagagataac gcgagaaacc
     8761 actccggcgc ttgattctca gagccacccg gttgcatcaa caatccgact attatcgagc
     8821 ataggccgac caacgcgcca tgcttcatct ccaaagttcc ggctttgtag gcggcgtaga
     8881 acccgccaat caacgtgccg agcgaaccga gcaagagact cgcgagtaag ccgtctgcgg
     8941 aaagccaata tttcgccatt tcctcctcga aaccctcggt gacgccagag ccctgggcta
     9001 cgtaaaagcg ataataaaat gccaccagcg caaacgtcgc gatgaagtcg acaaccgccc
     9061 ctgtgatgat cggccggatt tgaataccgg taaaaaaacc cgtcggctcg atgggtttgg
     9121 gcggtgaacc ggttcggtgt ggatcgtatt gcatcggtat gttatctgct aatgtcggcc
     9181 cagcgggcta cagttggcat cggacgattc tgcttttacg ggtgcgcgtt gacttggtcc
     9241 cgactgtcgg caaaataact ttaacccggg gccaaaggca accgatcggc gaaaatgttt
     9301 caccgctacg attatgaatc cgagtattat ccgagcttga gccgcttgcc tctcgacctg
     9361 cgccgcaagc tcgatattag cgggattaaa ctgtccctca aggactggct tgcgatcagc
     9421 ttggaagaac ggatggtgct ttgccactta ccctgcgact acgacgagga gcgccaggca
     9481 ttcgccactt acttagattt cctcgccaac aaatacctcg gtacaccggc ggaaaagacc
     9541 gcacccatga acatccggtc gtggagcgag tcggcagtgc caggcgccgt agcaaagcga
     9601 agtgcagcga cgggccaggc ggtgacgccg atcgaatggc gccgctggca atcgcaccat
     9661 cgctatgcgc tctacaagac ggcaacctcc aacaatcaac cggaagcctt tgagcgtgtt
     9721 ttgcggcaac tgcgccagct ggcgactcac tactaggagc ggtgatgaaa gcaacgacac
     9781 ttggcttgcc ggcgcggtac acttcggctt cagcggcaga cttgcgagaa ttatcgccga
     9841 aaacttagct gcgcctttta ggcacgctca tggccacatc ctaatcttaa aatacttgta
     9901 aattgacaaa gccatcatgt cactgatcgt cttcatcgcc atggtcgcga ccgcggctct
     9961 cgttggcatc caattcggcc ccggcccttg gtacctcgcc ctacagaagc cgtcatggac
    10021 gccacccaat tggatcttcg gaccggtgtg gaccgcgcta tatatcgcca tcgcggtcgc
    10081 cggttgggca gtatggcgat ccaaagctgt tagcattacc aagccgatcc tgctttggct
    10141 gcttcagtta attctgaacg gactct
//
LOCUS       DASQKS010000043         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2543812, whole genome shotgun sequence.
ACCESSION   DASQKS010000043 DASQKS010000000
VERSION     DASQKS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543812"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VE170_01095"
     CDS             complement(<1..610)
                     /locus_tag="VE170_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HYJ14053.1"
                     /translation="MANHTDSLGFYAAEVLQRTAARQPDKTAIIAKEEELTFAALNQR
                     VQALAGQLIAEGIGQGDRVGVLLPNSAAIPLAYYSTQKIGAVTVILDARLKGKELEGV
                     LQDADLKLLVVHQTLYPEVEEIFKSIAPIPVWIAGGTDERSFEQRFRAIAAVDNLPNL
                     KPDDDALILYTSGTTGEPKGVVLTYRNLLQYPMVMGAMNVCDA"
     gene            complement(586..1581)
                     /locus_tag="VE170_01100"
     CDS             complement(586..1581)
                     /locus_tag="VE170_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ14054.1"
                     /translation="MAKIDSEIIGWGKLLFERRLISGWGGNLSCRMGKNDFLITGQHA
                     PLGFLTANDLVHIRADGTPVKKSQRASSETPMHLAIYAGTDARAIVHVHPPMVLAFSL
                     SHDSFSPVSYEEKYTIGEVPVIPQDTPTVTKPEALVEALKYSPVAIIKGHGTVAIGKT
                     FQDAFLYTDLLEEAVHCQIFKNGVGAKSEVAASLMAPVTASGGVGKDYEIFSREHMTA
                     LVDRANGDSDFRQFGGDTSLTTSLTLLMENNHMAWTVQFVAGEITGLSEQSDGDFLIS
                     GEAAWWRAVFSNKIDAFLATQQGKLKLQRGELAQLSRWYKPFQRAFALWQTIPIR"
     gene            complement(1594..3111)
                     /locus_tag="VE170_01105"
     CDS             complement(1594..3111)
                     /locus_tag="VE170_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14055.1"
                     /translation="MSVVQSPLFEPVLWHDEGFRILDETQVPEKLVYIEVAEVAQALD
                     AVREMKTRAYGQVLTFLYSNALVAQQYQGNDPVPLRARLTQLTQQFCDVRPTFDFRGL
                     GSFVAEFFDKLPDNGAVGAAIARRAREFGGEITRARSARAKRTAAILADPARVLTHCN
                     ISGELVAVAAYCKQLGKDLSVIATETRPYLQGARLTAWELAQAGVKVSLIPDCAVGQV
                     MAQGEVNAVVVGADRVAQNGDVINKVGTYAIARMAKVYGVPFHALVQDPRSLARGADV
                     AIEERSGEELLIYHGRSLVAGEHADLKVRYPAFDLTPAEFVTNYVGLDGLYTAEAFRR
                     QYQVDATVDARVSRANAKYLLLYGVPPPSQYSFLLSALKAERAESLLVPEMRPELWGA
                     RVVVAELLKRKAPTTVIADNMMGTLFAQGEIGKLCFFYDSLVAAGAQGICGSLLAVQL
                     ARLHGVPIELFSGDCHKESTTDSDVTTFLGEPICPAGVTVRAVGSEVVPWALLKN"
     gene            complement(3138..3872)
                     /locus_tag="VE170_01110"
     CDS             complement(3138..3872)
                     /locus_tag="VE170_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14056.1"
                     /translation="MVKEPHFTPQRMLGMLGLKANSLGPYALIPGPKERSVMMVKLLD
                     APQKSFTFLDYEMHTGGLDGKRVSIGNGGRYAPDTAITTEILCGAGAESLIRVGSCGS
                     LQDHVKVGDLVIVTGALRGDGTSRYYVAENFSTVADPSVVAALKQAAEDLKVHCHLGW
                     IFTTDALFQETPELIHQLNQQNVSSIDMVTSTFLTIAQVRGKKAGAVLAVSDECLYGK
                     FGFRDPVFFEAEQKTIEVARQALKYL"
     gene            complement(3884..>4205)
                     /locus_tag="VE170_01115"
     CDS             complement(3884..>4205)
                     /locus_tag="VE170_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HYJ14057.1"
                     /translation="HDSCHLGRGCGVYDPPRQILRAIPGLNLVEMERNQRWAWCCGGG
                     GGVPETDSALAQWNAADRMREARASGAELMLTSSALCQRSFGDLPDGGLPTEDLLDFI
                     LAAL"
BASE COUNT          901 a         1289 c         1151 g          864 t
ORIGIN      
        1 aggcatcaca gacgttcatc gcgcccatca ccatcggata ctgcagcaaa ttgcgatagg
       61 ttagcaccac gcctttgggc tcgccggtgg tgcccgaggt ataaagaatc aaggcatcgt
      121 cgtcaggctt taagttgggc aaattgtcta ccgcagcaat ggcgcggaaa cgctgctcaa
      181 agctgcgctc gtccgtaccg ccggcgatcc agaccgggat cggcgcgatg gatttaaaaa
      241 tctcttcaac ttccggataa agcgtttgat gcaccaccaa aagttttaaa tcggcgtctt
      301 gcaacacgcc ttccagctct ttgcccttca ggcgcgcgtc gaggatcacg gtcacggcgc
      361 cgatcttctg ggttgaataa taagcgagcg gtatcgccgc agagttgggc aagagtacgc
      421 cgacccgatc gccttggccg atgccctctg cgatcaattg gccggccaac gcctgcacgc
      481 gctgattgag cgcagcgaaa gttagctcct cttctttggc gatgatcgcg gtcttgtccg
      541 gttgccgggc cgccgtccgc tgcaagactt ccgccgcgta aaaacctaac gaatcggtat
      601 ggtttgccat aacgcaaatg ccctctgaaa tggtttgtac cagcgcgaca actgcgccag
      661 ctcgccacgt tggagtttca acttaccctg ctgcgtcgcc aaaaaagcat cgatcttatt
      721 actgaacacc gcgcgccacc acgcggcctc gccgctaatg agaaaatcgc cgtccgattg
      781 ttcgctcaac ccggtaatct cgccagcgac gaactgcacc gtccaagcca tgtggttatt
      841 ttccatgagc aaggttagcg acgtagtcag actcgtgtcg ccgccaaact ggcgaaagtc
      901 gctgtcgccg ttggccctgt cgaccagcgc agtcatatgt tcgcgtgaaa aaatctcgta
      961 gtccttcccc accccgccgc tggccgtgac gggcgccatc aagctcgcag caacctcact
     1021 cttagcgccg acgccgtttt tgaaaatttg acaatgcacc gcttcctcga gcaggtcggt
     1081 gtagagaaac gcatcctgaa acgttttacc gatcgccacc gtgccgtggc ctttgatgat
     1141 cgcgaccgga gaatatttta gcgcctcgac cagcgcttcc ggtttggtta cagtcggcgt
     1201 gtcctgggga atcaccggca cctcgccgat ggtatatttt tcctcgtaac tcaccggtga
     1261 aaagctgtcg tgggacaacg agaacgccag caccatcggc gggtgcacat ggacgattgc
     1321 ccgagcgtcg gtgccagcgt atatcgccag gtgcattggc gtttcactcg aagcccgctg
     1381 gcttttcttg accggcgtgc cgtcggcgcg gatatgcacc aggtcattgg cggtcaaaaa
     1441 acccaacggc gcatgttggc cagtgatgag aaaatcgttt ttgcccatac ggcaactcaa
     1501 attgccgccc cagccactga tcagccggcg ctcgaagagc agcttgcccc agccgattat
     1561 ttcactgtca atcttagcca caggtaagcc tagctaattt ttcaataacg cccagggcac
     1621 gacttccgac ccgactgcgc ggaccgtgac gccggccgga caaatcggct caccgagaaa
     1681 agtcgtcaca tcgctatcag tggtcgattc tttgtgacaa tcgccgctaa acagctcgat
     1741 tgggacacca tgtaaacgcg ccaattgcac cgccagtagc gaaccgcaaa tcccttgcgc
     1801 tcctgcggcg accaagctgt catagaaaaa gcatagcttg ccaatctcac cctgggcaaa
     1861 caaagtgccc atcatgttgt cggcaatcac cgtggtcggc gccttgcgct tgagcaattc
     1921 tgctacgacc acgcgcgcgc cccatagctc cgggcgcatt tccggaacca acaggctttc
     1981 agctctttcc gccttcaatg cgctgagcaa aaaagaatat tggctcggtg gcggcacacc
     2041 gtaaagcagc agatattttg cgttggcgcg ggaaaccctc gcatcgaccg tggcatccac
     2101 ttggtattgc cgccgaaagg cctccgcggt gtagagaccg tccaagccga catagttggt
     2161 cacgaactcc gccggcgtca gatcgaacgc tgggtaacgc accttaagat cggcgtgctc
     2221 accggcgacc aacgagcggc catgatagat taatagttcc tcgcccgacc gctcttcgat
     2281 ggccacgtcg gcaccgcgcg cgagggaacg cggatcttgc accagcgcgt gaaacggcac
     2341 gccgtatact ttggccatgc gtgctatggc gtaggttccg actttgttga tcacatcgcc
     2401 gttttgcgcc acgcggtcgg cgccgacgac taccgcattg acttcgccct gcgccatcac
     2461 ctgcccgacc gcgcagtcgg ggatcaggga caccttcacg ccggcttgag ccaactccca
     2521 ggcggtcaac cgcgcaccct gcaagtaggg ccgggtctct gtggcgatga cggataaatc
     2581 tttgcccagc tgtttgcagt aagctgccac cgcgaccaac tcgccgctga tgttacaatg
     2641 ggtcaacact cgcgccggat cggctaggat cgcagcggtg cgtttagcgc gagcgctgcg
     2701 tgcccgggta atttccccgc cgaactcgcg cgcccggcgc gcaatagcag cgccaacggc
     2761 gccgttatcc ggtaacttat caaaaaactc agcgacgaag ctgcctaggc cgcgaaaatc
     2821 gaaggtcgga cggacgtcgc aaaattgttg ggttaactgc gtcaaccgcg cgcgcagcgg
     2881 caccggatca ttgccctggt attgctgcgc caccagcgcg ttgctgtaga gaaaggtcag
     2941 cacttggccg taggcgcggg tcttcatttc gcgcaccgca tcgagcgcct gggccacctc
     3001 agctacttca atgtacacga gcttttctgg aacctgagtc tcgtcaagaa tccggaagcc
     3061 ctcatcgtgc cagagcaccg gttcaaacag cggactttgg acgacgctca ttttcttagc
     3121 tcaacgaaaa gcaacggcta caaatatttg agggcttggc gcgctacttc gatggtcttc
     3181 tgctcggctt cgaaaaacac cggatcgcga aagccgaact tgccataaag acattcatcc
     3241 gacaccgcca gtaccgcgcc ggcttttttg ccgcgcacct gtgcgatggt caagaaagtt
     3301 gaggtcacca tgtcgatgct cgaaacgttt tgctggttca gttggtggat cagttccggc
     3361 gtctcttgaa acaatgcatc ggtggtgaat atccagccaa gatgacagtg aacttttaaa
     3421 tcctccgctg cctgcttcaa ggccgcgacg acgctagggt cggctacggt ggagaaattc
     3481 tcggcgacgt agtagcgcga ggtgccgtcg ccgcgcaggg caccggtgac gatcaccaga
     3541 tcgccgacct tgacgtgatc ctgcagtgag ccgcagctgc cgacacggat caaagattcc
     3601 gcgccggcgc cgcaaagaat ttccgtggta atcgccgtat ccggtgcgta acggccaccg
     3661 ttgccgatgc tgacccgctt gccgtcgagc ccgccggtgt gcatttcgta gtcgaggaaa
     3721 gtaaaacttt tttgcggcgc gtccaagagt ttcaccatca tcacactgcg ttccttcgga
     3781 ccgggtatca gtgcgtaggg accgagtgag ttcgccttga gtccgagcat gccgagcatc
     3841 cgttgcggtg tgaagtgcgg ttctttgacc ataaatcaaa acattaaagc gccgctaaaa
     3901 taaaatcgag cagatcttcc gtcggcaatc cgccatcggg caagtcgccg aaggaacgtt
     3961 ggcacagcgc gctcgaagtc aacatcagtt ctgcgccgct ggcgcgcgcc tcgcgcatgc
     4021 ggtcggctgc gttccactgc gctaacgcgg aatcggtctc gggcacgccg ccacctccgc
     4081 cgcaacacca agcccagcgt tggttgcgct ccatctcgac caaattcaga ccaggaattg
     4141 cccggagaat ttgccgcggc ggatcgtaga cgccgcagcc gcgaccaaga tggcaagaat
     4201 cgtgg
//
LOCUS       DASQKS010000044         9546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2544055, whole genome shotgun sequence.
ACCESSION   DASQKS010000044 DASQKS010000000
VERSION     DASQKS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544055"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VE170_01120"
     CDS             complement(<1..239)
                     /locus_tag="VE170_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYJ14058.1"
                     /translation="MIRKRSALSRRQFLTRGAAMAVGAAVGPYFLRNASAASRDRVTI
                     FHSSVADSIHPYNHSSSPIYGNWQHVIEPLIEYDVG"
     gene            complement(271..1110)
                     /locus_tag="VE170_01125"
     CDS             complement(271..1110)
                     /locus_tag="VE170_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010140748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14059.1"
                     /translation="MLSFRYLKIWVGGAILALLVVCGVAAPLIAPRDPQAQTLEDRLR
                     PPRWNENGLQAYLLGTDNLGRDMLSRIVYGSRISLMVGAATVIFAGLVGCTLGAIAGY
                     FGSTADEIVSKVSEIFLAFPFLLIAIAIMAFLGQGVGNLIMALMLSRWVQYCRVVRGE
                     VLSLKERDFVTAAKALGARDAYVIFHHVVPNTLASVTVIATFGMAIVIITEASLSFLG
                     LGVPVHIPTWGSMLAEGRSYINRAPWLTIFPGLAIFITVFGINLLGDGLRDIFDPKLR
                     RQR"
     gene            complement(1110..2108)
                     /locus_tag="VE170_01130"
     CDS             complement(1110..2108)
                     /locus_tag="VE170_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14060.1"
                     /translation="MNKLIRRLLGAIPVLLAISFLIFLLMHIAPGDPITLMLGDDATP
                     EDVARLRNELGLDKPIMVQYWDFLSRAVRGDFGRSLKFGEPVTKLVAERLPATLELAL
                     ASLFVAISIAVPLGVYSAIRHNSLVDHAGMSVALIGVSLPNFWLGIMLIYFLGGQLNL
                     LPVAGRIEYGIEIQPITGLYLLDCLITGNFAAMGSVAKHLLMPAFTLGTALAAIVTRI
                     SRSSVLEVMRQDYVTTARAKGLSEKTVIWRHILRNALITIVTILGLQLGALLSGSVVT
                     ETVFSFPGIGDLLIQSISARDYKLTQILILMFALTYFVVNLLVDFLYSWIDPRIKF"
     gene            complement(2167..3453)
                     /locus_tag="VE170_01135"
     CDS             complement(2167..3453)
                     /locus_tag="VE170_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYJ14061.1"
                     /translation="MDKKDILDKQKKYLFSCVATYYDEPLVIDHAKDHSVFDADGKEY
                     LDFFGGILTVSVGHCNDKVTKAIDGQSHKVQHMSTLYANEPQVNLAEKLAQITPGRLE
                     KSFFTNSGTEANETAVLLAQIYTKCQDVITLRHSYSGRSYLALSLSAQSSWRLMPNVV
                     PGVHHIANAYCYRCPFGLTYPSCDLKCAKDAEEAIQTMTSQGRVAAFLAEPIQGVGGF
                     IVPPKEYFKEIVGIIRKYGGLFIADEVQTGWGRTGGKMFGIEHWGVDPDIMTFAKGMA
                     NGVPIGATIATPEVADSMQGNTISTFGGNPVTCTAAHATINVIEEENLVENARVMGNR
                     LREGLDGLKAKYPVIGDVRGMGLMQGMELVGEQKKPDVNSTKRLMELTKNNGLLLGKG
                     GTYSNVIRVAPSLNVNQDEVDHALRVLDQSFGQLGQ"
     gene            complement(3486..4337)
                     /locus_tag="VE170_01140"
     CDS             complement(3486..4337)
                     /locus_tag="VE170_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ14062.1"
                     /translation="MEQLKPVKSGKTKVDDVNLYYEIYGQGDPLVFVAGTGISCAPWR
                     VSQVPEFAKHYQVVIYDHRGLGRSDKPDMPYSTELFAKDCAGLMDKLEIPRAHLLGHS
                     MGARVLQWVALKYPEKVRSLVLSGPGSGKYDESLDYPRGVPMDAALEMIEKGYEKYQS
                     DHWGPGFMFSESFMKEHPDVVKQYQDLIVDEVPPLKCYLRHVVARQCHETTHLIHNIK
                     APTLVIVGSKDTHEGGTGDHVASARVLAAKIPGAELHLVDGGRHGYLREMPEKGHPPI
                     LDFLKRH"
     gene            4515..5585
                     /locus_tag="VE170_01145"
     CDS             4515..5585
                     /locus_tag="VE170_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14063.1"
                     /translation="MKSYFTGLFVVAMLIVTGPALYAGAAPESAGQLLARLNKLPAEA
                     RHKQLVEKARAEGEVSFYSSLQAQQIEPFIRVFQKRYPFIKVNPYRVSGNRQVIKIQT
                     EMNGGNHLFDVTNGSAEQASAIKKIGAIDPYFSPQRDFYNPPNRDKDGFFASLYVIPI
                     VLGYNTNMVKRQDAPKTYEELLQPKWKSNMFLDDEAYEWSAVLQKHMGRDKALQYMRA
                     LAKQDLRLMRGRTAQSQLLGAGERPIAIVLSGHTVLDMKARGAPVDQVILDPYFAQAN
                     KLMLARHAPHPHAAALFYDWALSEEGQGIITGFGRVVARKGVKQRFPELVEKESFLVD
                     VDFIGPIMDQTGKEFSQIFLGR"
     gene            5611..6684
                     /locus_tag="VE170_01150"
     CDS             5611..6684
                     /locus_tag="VE170_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14064.1"
                     /translation="MLKKLFLVLVTLLAIASHQTAVLAGAAPETAGQILARLSKFAPE
                     QRQAVLVEKAKVEGEVNFYSTLQAQQIEPFLQVFRKRYPFVKPVPTRVSGNRQLVRIQ
                     SEFNSGAHIVDVANGSPELAAGLRKLGIIDPYHSPQRDFYVAPSKDKDGYFTSFYVMP
                     IVLGYNSQQVKRADAPKNYEVLTQPKWQGNIMLDDEALEWFAVMLRHLGREKGLQYMR
                     ALAKQDLRMVRGRTAQSQLLAAGERAIAINVSGPTTLDLKARGAPVDQVIVDPYFSQP
                     NKMVLARHAPHPHAAALFLDWALSEEGQSTVTTFGRVSARKGVKQRFPELMEKEIFHA
                     DVEFISPIMEQTAKEFGQIFLGR"
     gene            complement(6692..7384)
                     /locus_tag="VE170_01155"
     CDS             complement(6692..7384)
                     /locus_tag="VE170_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008555403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HYJ14065.1"
                     /translation="MILEVAVRWSEILMGAAFLQQSAEHLTAPANERRLHILRMVLAF
                     LLIAGVQSAIIEALLVLLGIATLYRFQGPYNGGSDRMSLLLLFCLLAARLAPTRQWQE
                     IALGYLAVQILLSYAISGWVKLTNPDWRTGQALQDIFQFSVYPVSERLRSLAMAPTLL
                     LALSWTFMLFELLFPLALLDARYLKTALVIAGLFHLVNGCVFGLNRFLWIWVAAYPCL
                     LWFQQRVVPSAF"
     gene            complement(7381..7848)
                     /locus_tag="VE170_01160"
     CDS             complement(7381..7848)
                     /locus_tag="VE170_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14066.1"
                     /translation="MKAVRLLFSYLLPALIPSWRFFDYIAPSPRIEFAVAAAADDPAM
                     RWREFRPRPVHLPFAAMLRRLLWNPLWNESLFMASCAEKLLDEPSARRETELFTRIVA
                     AIARGETGDQITASTYLRFRIVVLKREGEEISRRVGFVSPAQRVDASGRIGVP"
     gene            complement(7845..8798)
                     /locus_tag="VE170_01165"
     CDS             complement(7845..8798)
                     /locus_tag="VE170_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HYJ14067.1"
                     /translation="MAGTSLLALIDDIASILDDVAAMSKVAVKKTAGVLGDDLALNAH
                     QVAGVRTDRELPVVWAVAKGSLKNKVILVPAALAISILIPWAITPLLMIGGAYLCYEG
                     FEKIAHKFIHDPSTEAHQKEHLQALINPAVDLVAVEREKIQGAIRTDFILSAEIVVIA
                     LGTVATAAFGKQVAVLCAIAVFMTVGVYGLVAGIVKLDDAGLHLSRRSGKGPWREFQR
                     GAGHLLVQLAPYLLKSLSIVGTIAMFMVGGGILTHGIPGAHEWIEHVAHGAGGIPAVG
                     AVLETILPYLMDIMCGVLAGFLVLLGVSAATRVFRMFKGAR"
     gene            complement(8914..>9546)
                     /locus_tag="VE170_01170"
     CDS             complement(8914..>9546)
                     /locus_tag="VE170_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14068.1"
                     /translation="VSFHKNWARVFVEQVIKADNQKYAGKSVADVAVLRKQDGLDAFL
                     DLALEEDLGTTFETTNRGFNPEAMSTIMKSPYVVIGTSDAGAHVQFGADFGYCTTLLG
                     MWVRDRQMIGLEQAIHKLTFHVASIWGIEGRGLLRPGFHADVTVFDAKTIKACEPEWA
                     EDYPAATKRLIQRAEGVHYTIVNGKVIYHDGKLSGEMAGYVLRSAAYAEQ"
BASE COUNT         2171 a         2855 c         2638 g         1882 t
ORIGIN      
        1 ccgacatcgt actcgatcaa aggctcgatg acatgctgcc aattgccgta aatcggactc
       61 gagctatggt tataaggatg aatcgaatcg gccacgctcg aatgaaagat cgtcacgcgg
      121 tcgcgtgagg cggcagacgc attgcgcaag aaatacggcc cgacggccgc gcccaccgcc
      181 atcgcggcgc cccgtgtgag aaattgccgc cgactgagcg ctgatcgttt cctaatcata
      241 aactctctcc tgtgactgta tcggtatcgt ttatctctgt cgtcgtagct tgggatcgaa
      301 aatatcgcgt aagccatcgc ccaaaagatt gatgccgaat accgtaatga aaatcgccaa
      361 gccgggaaag atcgtcagcc agggcgcacg attgatgtaa ctgcgtccct ccgcaagcat
      421 cgaaccccag gtcggaatgt gcacgggcac accgaggcca aggaagctca ggctcgcttc
      481 ggttatgatg acgatggcca tgccaaaagt ggcgattact gtgacgctcg ccagggtatt
      541 gggaaccaca tggtgaaaaa tcacgtaagc gtctcgggcg cccaaggcct tagccgcagt
      601 gacgaaatcg cgctccttca acgacaacac ttcgccgcgc acgacccggc aatattgcac
      661 ccagcggctg agcatcagcg ccatgatgag attaccaacg ccttggccaa ggaaggccat
      721 tatagctata gcgatcaaga gaaacggaaa ggcgagaaag atttccgata ccttgctgac
      781 gatttcatca gcggtgctgc caaaatatcc ggcaatggcg ccgagcgtgc agccgaccag
      841 tccggcgaaa atcactgtcg ccgcgccgac catcagcgag atccgcgatc cgtaaacaat
      901 ccggctcaac atatcgcggc cgagattgtc ggtgccgagc agataggcct gcaacccgtt
      961 ttcgttccag cgcggcgggc gcagccggtc ctccaaggtt tgagcctgcg gatcgcgcgg
     1021 cgcgatcaac ggcgcggcga cgccgcacac caccagcagt gccaagatcg ctccgcccac
     1081 ccaaatctta agataacgaa aagacaacat cagaacttga tccttggatc gatccaactg
     1141 tagagaaagt cgaccaacag gttaaccacg aagtaagtta gcgcgaacat cagaatcaat
     1201 atttgggtaa gtttatagtc gcgcgccgaa atcgactgta tcaacagatc gccaatgccg
     1261 ggaaatgaaa ataccgtttc ggtgaccacc gaaccgctga gtaaagcgcc caactgaaga
     1321 ccgagaatcg ttacgatggt gatcaatgca ttgcgcagga tgtgcctcca gatcacggtc
     1381 ttttcgctca gccccttggc gcgcgcggtg gtcacgtagt cctggcgcat aacttcgagg
     1441 acgcttgaac gggaaatccg tgtgacgatg gccgcgagcg cggtgccgag cgtaaacgcc
     1501 ggcatcagca aatgcttggc cacactaccc atcgccgcaa aatttccggt tattaaacaa
     1561 tcgaggagat agagcccggt aatcggttgg atctcgatgc cgtactcgat gcggccggcc
     1621 accggcaaaa gattgagctg gccgccgaga aagtaaatca gcatgatgcc caaccagaag
     1681 ttcggcaaag atacgccgat caacgccacg ctcatgccgg catggtcgac cagcgaattg
     1741 tggcgaatcg ccgagtagac ccccagtggc acggcgatgc taatcgccac gaataaactc
     1801 gcaagggcga gttccaaggt cgccggcagc cgctcggcga ccaacttggt caccggctcg
     1861 ccaaacttga gcgaccggcc aaagtcgccg cgcacggcgc gggaaagaaa atcccagtac
     1921 tggaccatga tcggcttatc caagccgagc tcattgcgca gcctggcgac atcttcgggc
     1981 gtggcatcgt cgccgagcat caaggtgatc ggatctcccg gcgcgatatg cattaagaga
     2041 aaaatcagaa aagagatggc cagcagaact ggaatcgcac cgagcaatct gcgaatgagt
     2101 ttgttcatgt ccgacgcggg ctagaaataa gtcgatttag cgacaacatg gcataagcca
     2161 ataccattac tgccccagct ggccaaacga ctgatccaaa acccgcaggg cgtgatcgac
     2221 ttcgtcctgg ttgacgttca atgacggcgc gacgcgaatg acattgctgt aggtgccgcc
     2281 cttgccaagc aaaaggccat tgtttttggt cagttccatc aagcgtttgg tgctgttgac
     2341 atcgggcttc ttctgttcgc cgaccagctc cataccttgc atcaagccca tgccgcggac
     2401 atcgccgatc accggatact tggccttcag cccgtccaat ccctcgcgca accggttgcc
     2461 catcacccgc gcgttctcga ccaggttttc ctcttcgatg acgttgatcg tcgcatgcgc
     2521 cgcggtgcag gtcaccggat tgccgccgaa agtcgatatc gtattgccct gcatgctgtc
     2581 ggccacttcc ggtgtcgcaa tggtagcgcc gatgggcacg ccgttggcca tgcccttcgc
     2641 aaaagtcatg atgtcggggt cgacgcccca atgctcgatg ccgaacatct tgccgccggt
     2701 tcgtccccaa ccggtctgca cttcgtcggc gatgaacagg ccgccatatt ttcgaatgat
     2761 gccgacgatc tctttaaagt attccttcgg cggtacgatg aaaccgccga cgccctgaat
     2821 cggttcggcg agaaaagccg ccactctgcc ctgggaagtc atggtttgaa tcgcctcttc
     2881 ggcgtccttg gcgcacttca aatcgcagct cggataagtc agtccgaacg gacaacgata
     2941 gcagtaagcg ttggcgatat gatgcacacc ggggacgaca ttgggcatca agcgccagct
     3001 cgactgcgcc gacagactca aggccaaata ggagcggccg ctgtaactgt ggcgcagggt
     3061 aattacgtcc tggcacttgg tatagatctg cgccagcagc accgcggtct cgttggcttc
     3121 ggtgccgctg ttggtgaaaa aggatttttc taaccggccc ggcgtaatct gtgccaactt
     3181 ctcggccaag ttgacctggg gctcgttggc atagagcgtc gacatatgtt ggaccttgtg
     3241 actctgcccg tcgatcgcct tggttacttt gtcattgcaa tggccgacgc tgaccgtgag
     3301 gatgccgccg aagaaatcca gatactcttt gccgtccgca tcgaacaccg agtgatcctt
     3361 ggcgtggtcg atgaccaagg gttcgtcgta gtaagtcgcg acgcaggaga atagatattt
     3421 tttctgcttg tcgagaatgt cttttttatc catagaagcc tgtcagtacc gtgtgaatcc
     3481 cagacttaat gacgtttcag aaaatccagg atcggcggat ggcccttttc cggcatctcc
     3541 cgcaggtaac catgtcggcc gccgtcgacc aggtggagtt cggcgccggg aatcttcgcg
     3601 gccagcaccc gtgctgacgc cacatgatcg ccggtgccgc cttcgtgggt atctttgctg
     3661 ccgacgatga ccaaagtcgg cgctttaatg ttgtgaatca agtgggtggt ctcatggcac
     3721 tggcgcgcca ccacatggcg cagatagcat tttaacggcg gcacttcgtc gacgatcaaa
     3781 tcctggtact gcttgaccac atccgggtgc tctttcatga aactctcact gaacatgaat
     3841 ccgggtcccc agtgatcgct ctgatacttt tcgtagcctt tttcgatcat ctccagcgcc
     3901 gcatccatcg gcacgccgcg cggatagtcg agcgactcgt catatttccc cgagcccggc
     3961 ccggaaagaa ccaggctgcg gactttttcc ggatacttca gcgcgaccca ctgcaacacc
     4021 cgcgcaccca tggagtggcc caatagatgt gctcgcggga tttcgagctt atccatcaat
     4081 ccggcgcagt ccttggcgaa cagttcggtg gagtatggca tatccggctt gtcactgcgg
     4141 cccaggccgc gatgatcgta aatcaccact tgatagtgct tggcgaactc cggcacctgc
     4201 gatacccgcc agggcgcgca actgatgccg gtaccggcga caaacaccaa ggggtcgccc
     4261 tggccataaa tctcgtagta gagattgaca tcgtcgacct tggtcttgcc actcttgacc
     4321 ggtttcaact gttccatcgg gcctccaacc tgccgccagc taatcgccgt gacgctatcg
     4381 aatcgttaaa ttagagtcaa tatgagtttg ccccggacag cgccatgata atcctcgctt
     4441 gacagcttca cggcgcgctg ggataaaagt ggcggactgc aatgggtacg ccggaatagc
     4501 atggaaggtg agcgatgaaa agctatttta cgggtttgtt cgtcgtcgcg atgctaatcg
     4561 tcactgggcc agcgctctat gccggcgccg ccccggaaag cgccggccag ctcctcgccc
     4621 gcttgaataa gctaccggct gaggcgcgcc acaagcagct ggttgagaaa gccagagccg
     4681 aaggtgaagt gtctttctat agctcgctgc aagcgcagca gatcgagccg tttatccgag
     4741 tgtttcaaaa gcgctatccg ttcatcaagg tcaatcccta tcgggtctcc ggcaaccgcc
     4801 aagtaatcaa gatccagacc gagatgaacg gcggcaacca tttgttcgac gtcaccaacg
     4861 ggtccgccga gcaggcctcg gcgatcaaga agatcggcgc catcgatccc tacttctcgc
     4921 cgcagcgcga tttctacaac ccgccgaatc gcgacaaaga tggtttcttc gcttcgctgt
     4981 acgtcattcc catcgtgctc ggctacaaca ccaacatggt caagcgccag gatgcaccga
     5041 aaacctacga agagctgctc cagcccaagt ggaaaagcaa catgtttctc gacgacgagg
     5101 cctatgagtg gtccgccgtg ttgcaaaagc atatgggccg ggacaaggcg ctgcaataca
     5161 tgcgcgcgct ggccaagcag gatttgcgat tgatgcgcgg acgcaccgcg cagagtcagc
     5221 tcctgggcgc cggcgaacgc cccatcgcca tcgtgctgtc gggtcacacg gtgctcgata
     5281 tgaaagcgcg cggcgccccc gtcgatcaag tcattctcga tccctacttc gcccaggcca
     5341 ataaactaat gctggcgcgc cacgcgcccc atccccatgc cgctgcgctg ttctacgact
     5401 gggcgctgtc ggaagaaggc caaggaatta ttaccggctt cggcagggtc gtggcgcgca
     5461 aaggcgtcaa acaaagattt cccgagttag tcgagaaaga atcgtttctc gtcgacgtcg
     5521 acttcatcgg cccgatcatg gatcagaccg gcaaggagtt cagccagatt ttcttgggcc
     5581 gatgaattcc ctcaagctta ggagcggtcg atgttaaaga aactgtttct agtcttagtc
     5641 acgctgctag cgatcgcatc ccaccagaca gcagtcttgg cgggcgccgc acccgagacc
     5701 gccggccaaa tcctcgcccg gttgagcaaa tttgcgccgg agcaacggca agcggtgctg
     5761 gtagagaaag ctaaggtcga aggcgaagta aatttctaca gcaccttgca agcccaacag
     5821 atcgagccgt tcttgcaggt cttccgcaag cgctatccgt tcgtgaaacc cgtgcccacg
     5881 cgggtttccg gcaaccgcca gctggtgcga attcagagtg aattcaattc cggcgcgcat
     5941 atcgtcgatg tcgcaaacgg ctcgcccgaa ttggcggcgg gactgagaaa actcggcatc
     6001 atcgatccgt accattcgcc acagcgcgac ttttatgtcg cgccgagcaa ggacaaggac
     6061 ggctacttca cgtcgtttta tgtcatgccc atcgtcctcg gttacaattc ccagcaggtc
     6121 aagcgcgccg acgcgccgaa gaattacgag gtgctgacgc aaccgaaatg gcaaggcaat
     6181 atcatgctcg acgacgaggc cctggaatgg ttcgccgtca tgctcagaca tttgggccgg
     6241 gaaaaaggcc ttcagtatat gcgcgccctg gccaaacagg atttacgcat ggtgcgcggc
     6301 cggacggcgc agagccaact gctcgccgcc ggcgaacgtg ccatcgccat caacgtctcg
     6361 ggcccgacca ccttggatct caaagcgcgc ggcgcgccgg tggatcaggt gatcgtcgac
     6421 ccctattttt cccaacccaa caaaatggtc ttggcccgcc acgcgcctca tcctcacgcc
     6481 gccgccttgt tcctcgactg ggcgctgtcc gaagaaggcc aatccacggt caccactttc
     6541 ggccgcgtct ccgcccgcaa aggcgtcaaa cagcgctttc ccgagttgat ggaaaaggaa
     6601 atcttccacg ccgatgtcga gttcatcagt ccgatcatgg agcaaacggc gaaagagttt
     6661 gggcaaatct tcttgggacg gtgaaaagcg atcagaaagc gctcggaaca acccgctgct
     6721 gaaaccacag cagacaagga taagccgcaa cccagatcca caaaaaacga ttgaggccga
     6781 agacgcagcc attgaccagg tgaaataacc cggcaatgac taaagccgtt ttcaagtacc
     6841 tagcatcgag tagcgcgagc gggaagagta attcaaacag catgaaagtc catgacagcg
     6901 caagcagcag agtcggtgcc atcgccaggg agcggagacg ctcactgacc ggatacaccg
     6961 agaattggaa aatgtcttgc aacgcttgcc cggtgcgcca atccgggttg gtcaatttga
     7021 cccaacccga gatcgcatag gacaacaaga tctgcaccgc cagatatccc agggcgattt
     7081 cttgccactg ccgggtcggc gcgagacgcg ccgccaatag acagaacagc agcaacaggc
     7141 tcatgcggtc gctgccgccg ttatacggcc cctgaaagcg atacagggtg gcaatgccta
     7201 gaagcacaag caaggcttcg atgatagccg attggacgcc ggcgatcagc aaaaacgcca
     7261 ggaccattcg caggatgtgc agccggcgtt cgttcgccgg agcggttaga tgctcggcgc
     7321 tttgctggag aaacgccgcg cccatgagga tttcgctcca gcgcactgca acttctaaaa
     7381 tcatggaacg ccgatcctgc cgctggcgtc gacccgctgg gccggggaga caaagccgac
     7441 ccgcctggaa atctcctcgc cttcacgctt tagcactacg atacggaacc tcagataagt
     7501 cgaagccgta atctggtcgc cggtttcacc gcgtgcaatg gcggcgacga tgcgggtaaa
     7561 cagctcggtt tcgcgccggg ccgacggttc atcgagcagc ttctccgcac agctcgccat
     7621 gaatagggat tcattccaga gcggattcca cagcaaccgg cgcagcatcg cggcgaacgg
     7681 caaatggacc ggccgcgggc gaaactctcg ccagcgcatc gccggatcgt ccgctgccgc
     7741 ggcgacggcg aactcgatgc gcggcgacgg cgcgatgtag tcaaagaacc gccaggatgg
     7801 gatcaacgcc gggagcaggt aactgaatag cagccgaacg gctttcaacg ggctcctttg
     7861 aacatgcgaa atactcttgt cgcggcggat accccaagca aaactaaaaa gccggcaagc
     7921 acgccgcaca taatatccat cagatacggc agaatagttt caagtactgc gcctacggcc
     7981 ggaatcccgc cggcgccatg ggctacgtgt tcgatccact catgggcgcc ggggatgcca
     8041 tgggtcagga tgccgccgcc gaccatgaac atggcgatgg tgccgacgat cgagagactc
     8101 ttcagcaggt aaggcgctag ctgcaccaat agatggccgg cgccgcgctg gaattctcgc
     8161 caagggcctt tgccgctcct ccggctcaag tgcagcccgg cgtcgtcaag cttgacgata
     8221 ccggcgacga ggccatagac cccgacggtc atgaagacgg cgatggcgca aagcaccgcg
     8281 acctgcttgc cgaacgctgc ggtcgctacg gtgcccaacg cgatcacgac gatctcagcg
     8341 gacagaataa agtcggtgcg gatcgcgccc tggatttttt ctctctcgac ggcgaccaaa
     8401 tcgacggctg ggttgatcag cgcttgcaaa tgttctttct gatgggcttc tgtcgacggg
     8461 tcatgaataa acttgtgggc gatcttttcg aatccttcgt agcaaagata ggcgccgccg
     8521 atcatcaata gcggtgtgat cgcccagggt atcaatatgc tgatcgccag tgccgccggc
     8581 acgagaatca ccttattctt gagcgatcct ttggccaccg cccagacaac gggcagttcg
     8641 cggtcggtgc gcacgccggc cacttgatgg gcattgagcg ccagatcgtc gcccaacacc
     8701 ccggcggtct tcttcacggc gactttgctc atcgccgcga cgtcgtcgag gatcgaggcg
     8761 atatcgtcga tcagcgctaa taaactagtt cccgccacgc gtaactccca gaccgtttac
     8821 cggcacataa aaagagggcg ggtttcaaac ccgcccttag atatttcaat tttttttcgg
     8881 ccttggcgtt gtatgcgggc aatccacgct cgttcactgc tcggcatacg ctgcactgcg
     8941 cagcacgtaa cccgccatct cgccgctcaa cttgccgtcg tggtaaatca ccttgccgtt
     9001 gacgatggtg tagtgcacac cttcggcgcg ttggatcaac cgcttggtcg ccgctggata
     9061 atcctcggcc cattccggct cgcaggcttt gatcgtcttg gcatcgaaga ccgtcacgtc
     9121 ggcgtggaat cccggccgca gtaaaccgcg gccttcgata ccccaaatcg acgccacatg
     9181 gaatgtcagc ttgtggatcg cctgttcgag cccgatcatc tgccggtcgc gaacccacat
     9241 gccgagcagc gtcgtacagt aaccgaagtc ggcgccgaac tggacgtggg ccccggcatc
     9301 cgaggtgccg ataaccacgt agggactctt catgatcgtg ctcatggcct cggggttgaa
     9361 accgcgattg gtggtctcga aggtcgtgcc caaatcttct tccagcgcca aatcgagaaa
     9421 cgcatcgagc ccatcctgct tgcgcagcac cgcgacatca gcgacgcttt tgccggcgta
     9481 cttttgatta tccgctttga tcacctgctc gacaaacacc cgtgcccagt ttttatgaaa
     9541 cgacac
//
LOCUS       DASQKS010000045         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1284556, whole genome shotgun sequence.
ACCESSION   DASQKS010000045 DASQKS010000000
VERSION     DASQKS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284556"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VE170_01175"
     CDS             complement(<1..1105)
                     /locus_tag="VE170_01175"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ14069.1"
                     /translation="MAFESMGDFITAAERVGEVLHVEGADLEMDVGGLTELTAEKNGP
                     MVVFDKFAGYPAGFRVAANANRTLRRFALAMELPLDIHPLELLRRWRDKRADSKPIAA
                     RVVKDGPVLANVQQGDAVNIEKFPAPRWHPGDGGRYIGTQDMVIVRDPEQGWVNMGCY
                     RAMVQRRDRVSLWINPQKHGRILAQKYWRDGKAAPVAVVFGCEPVTWMTASMSPPFGT
                     SEYELAGAYRGSPVDVVELPLTKLPVPAHAEIVIEGEIPPESEETAVEGPFGEWPGYY
                     THQGPEAVVRIKRIYYRDNPIIAGAPPLRPINWSNFTNYVHLWEHLERSGITDINGVW
                     GFYNGLLTVVSLKQRYAGHAKQALITAAGFRHGD"
     gene            complement(1137..1859)
                     /locus_tag="VE170_01180"
     CDS             complement(1137..1859)
                     /locus_tag="VE170_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14070.1"
                     /translation="MIRCTDIGKVYQQGESRITALAGVSLEIPKGAFAAVMGQSGSGK
                     STLLHLIGGLDRPSSGELLVDGRLIAQMADDQVTLFRRSQIGFIFQFFNLLPTLTAAE
                     NIALPFVLDGRSKAEADARAMALLAKVGLQHRQQHLPEALSGGEIQRVAIARALAFNP
                     PILLADEPTGNLDSQTGSAILDLLRQVNRHDGCTIVMVTHSEEAAGYGDRRIYLRDGR
                     VERVVENHTKEPAQGITSPLHS"
     gene            complement(1872..2237)
                     /locus_tag="VE170_01185"
     CDS             complement(1872..2237)
                     /locus_tag="VE170_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HYJ14071.1"
                     /translation="MELQDDPRFIKGIEEFNNGLYFECHETLEEIWLEEHGEERKFYQ
                     GIIQIAAGYYKLEQGVPVGALKLWRMGIEKLQAYSPVYLGVNVDALVAAVGANLAALE
                     KDQGQTAKALGVPTIQLFC"
     gene            2408..>3104
                     /locus_tag="VE170_01190"
     CDS             2408..>3104
                     /locus_tag="VE170_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HYJ14072.1"
                     /translation="MITEDSSQPIAKTELELDIDRLSYGPYGIGRSGGKAVMIPNSAP
                     GDRVIARLIDSKERYAIGAIVNIVAPSPLRQTPPCPYVGDCGGCTWQHIRYREQLKAK
                     EQSVADALRRIGKLDGFELRPIIAAPGAEHYRRRIRLQVNAVKRLGFYSAASHHVVEV
                     DACLIAAQPLNAAIAPLRLWLDSLAANLEHIEIISGDEPGQTVVSATATEPLIPNDEE
                     RCEQLVGSTDAIDG"
BASE COUNT          608 a          974 c          880 g          642 t
ORIGIN      
        1 tgtcgccgtg gcgaaagccc gcggcggtga tcaaggcttg cttggcatgg ccggcgtagc
       61 gttgcttcaa cgacaccacg gtgagcagac cgttgtaaaa accccagacg ccgttgatgt
      121 cggtgatgcc ggagcgttcg agatgttccc acaaatggac gtagttggtg aagttgctcc
      181 agttgatcgg ccgcagcggc ggcgccccgg cgatgatcgg attgtcacga tagtaaatcc
      241 gcttgatgcg caccaccgct tccggtccct gatgggtgta gtagcccggc cactcgccga
      301 acggtccttc caccgcggtc tcttcgctct ccggcggtat ttcgccttcg atgacgatct
      361 cggcgtgcgc cggcacgggc agcttggtca gaggcagctc gacgacgtcc accgggctgc
      421 cgcggtaagc gccggccagt tcgtattccg aggtaccgaa gggcggcgac atcgacgccg
      481 tcatccaagt caccggctcg cagccgaaca ccaccgccac cggcgcggcc ttgccatcgc
      541 gccaatattt ttgcgccaga atacgcccat gcttttgcgg attgatccac aggctgaccc
      601 ggtcgcggcg ctgcaccata gcgcgataac agcccatgtt gacccagcct tgttcggggt
      661 cgcggacaat caccatatct tgggtgccga tgtagcggcc gccgtcgccg ggatgccagc
      721 gcggcgcggg gaacttttca atattaacgg cgtcgccttg ctgcacattg gccagcaccg
      781 gtccgtcttt gaccacccgc gcagcaatcg gttttgagtc cgcccgcttg tcgcgccagc
      841 gccgtagcaa ttccaacgga tgaatgtcga gaggcaactc catggccaaa gcaaaacggc
      901 gcagggtgcg gttggcgttg gcggcaacgc gaaagcccgc gggatagccg gcgaacttgt
      961 cgaacaccac catcggcccg tttttttccg ccgtcaattc ggtcaaccca ccgacatcca
     1021 tctccaggtc cgcgccttcg acgtgcagta cctcgccgac ccgttccgcc gcggtgataa
     1081 aatcacccat cgattcaaat gccatgtttc ctccagaaag tcggaattgt tgggaattaa
     1141 gagtggagcg ggcttgttat accttgcgcc ggctccttcg tgtgattttc tacgacccgc
     1201 tccacccgcc catcgcgcaa ataaatcctg cgatcgccgt atcccgcggc ttcttcgcta
     1261 tgggtcacca tgacgatggt gcaaccgtcg tggcgattga cctggcgcaa taggtcgagg
     1321 atagcgctgc cggtttgcga atcgaggttg ccggtgggtt cgtcggcgag caggatcggc
     1381 gggttaaacg ccagcgcccg ggcgatcgcc acccgctgga tctcgccgcc ggaaagcgcc
     1441 tcagggaggt gctgctggcg atgctgcaaa ccgactttgg ccagcagcgc catggcgcgc
     1501 gcgtccgcct ccgccttgct ccggccgtcg agcacaaagg gcagcgcgat gttttccgcc
     1561 gcggttaacg tcggcagtag attaaaaaat tgaaagatga aaccgatttg gctgcggcga
     1621 aacagcgtca cttgatcgtc cgccatctgt gcgatcaacc ggccatcgac gagcaactca
     1681 ccgctgcttg gccggtccaa gccgccgatc aaatgcagca acgtgctttt ccccgaaccg
     1741 ctctggccca tcaccgccgc gaacgcgccc ttgggaatct ccagcgacac cccggcaagg
     1801 gcggtgatgc gactctcgcc ctgctgatag actttgccga tatcggtgca gcgaatcatg
     1861 aggcggcaga gttaacagaa cagctggata gtcggcacgc ccaaagcctt ggcggtttgc
     1921 ccttgatctt tttccaaagc ggcaagattg gcgccgaccg cagccaccag cgcgtcgacg
     1981 ttgacgccaa gataaaccgg actgtaggcc tggagttttt cgatacccat acgccagagc
     2041 ttgagcgcgc ccacggggac gccctgttcg agcttgtaat aaccggcggc gatttgaatg
     2101 atgccttgat aaaatttgcg ctcctcgcca tgctcctcga gccagatctc ttcaagggtc
     2161 tcgtgacact caaaatacag tccattgttg aactcttcga tgccttttat aaacctcgga
     2221 tcgtcttgta gttccataat tgctgctatg tggaataaac taaggggtcg gctttaaaaa
     2281 tcgccccggt aaatcgcgat tctttttttg tgacctttgc gttctttgcg gcggattttc
     2341 cggttccgag atcatcttgg ttttcgccat tgccgcccca gcccctttat gttttagttg
     2401 ccacactttg attaccgaag acagctcaca acctatcgcg aaaaccgaac tagaactaga
     2461 tatcgacagg ctttcctacg gcccctacgg catcggccga agcggcggca aagcggtcat
     2521 gattcccaac agcgctcccg gcgatcgcgt gatcgcccgg ctcatcgact ccaaagagcg
     2581 ctacgccatc ggtgcgatcg taaatatcgt cgcgccatcg cctttgcgcc agactccgcc
     2641 ttgtccctac gtcggcgatt gcggcgggtg cacctggcaa catatccgtt accgggagca
     2701 actcaaggcc aaagagcaga gcgtcgcgga cgccctgcgg cgcatcggca agcttgacgg
     2761 ttttgaactg cgccccatta tcgccgcgcc cggcgccgag cattatcgcc ggcgcattcg
     2821 cttgcaagtc aacgcggtaa aacgtttggg gttttacagc gccgcctcgc accatgtggt
     2881 cgaagtcgat gcttgtctga tcgccgcgca gccgttgaac gcagccatcg cgccgctgcg
     2941 cctgtggctc gacagtctcg ctgccaacct cgagcatatc gagatcatca gcggcgacga
     3001 acccggccaa actgtcgtca gcgcaaccgc caccgagcca ttgataccga atgatgagga
     3061 acgctgcgag caactggtcg gtagtacgga tgccatcgat ggcg
//
LOCUS       DASQKS010000046         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2544718, whole genome shotgun sequence.
ACCESSION   DASQKS010000046 DASQKS010000000
VERSION     DASQKS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544718"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1589
                     /locus_tag="VE170_01195"
     CDS             48..1589
                     /locus_tag="VE170_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ14073.1"
                     /translation="MDSSLFDAAIKGFVNILHLKVFFAMAMGVGIGTFTAVAPQGLGM
                     PLVYAIALPVVIKWEPVTGIAFLIGASSVSAICAAYLPILFGIPGGSGSQATVLDGYP
                     MGKKGEGRRALGASFMAGGMGALIGTLTLMVAIPAAKPLIYLMGSPELFVIMLWGLSM
                     VAVLAGSRPLKGLIATALGLMLATVGQQAQSGVMRFVYDQPYLLDGMSISIMALALFG
                     VPSALDLALTKLGVEQQPVPLKGSLFDGVRDALREWWLVLRCSFVGVWVGIVPGLGSQ
                     VVDWLAYGHAAQTCKGGRETFGTGDVRGVIAPESANDAKDGGDLVTTLLLGFPQGVVT
                     ALFIVALLAWGFVPGPEMIKKNPEVIYSVVWLQGISGIVGTLVGFCLASQLAKLAEVR
                     YTFMVPIMFMFILMGAFSVNRDPLDLVVVVGFGILGYFMRRAGYPRPAMILGLVLGDL
                     MEKYLYRSVASYGFSWMTRPAVIVIFIIAAVSLFFTLRGKLKASKKDSTATFSGAIAR
                     SDNED"
     gene            1579..2046
                     /locus_tag="VE170_01200"
     CDS             1579..2046
                     /locus_tag="VE170_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ14074.1"
                     /translation="MKTNFALFLLIIFSVAIASGWGWPDIAKIMPVYVAAIPGLLMVM
                     VQIFREATGWETRRITTSIDMDESGQNQLDEATARKRTLAFFAWFIAGALGIWLIGIV
                     YALPVLVFLYALLQGKEKILTSLVIGGCAYLLIWGLFEYMLETRWPAGLLFGY"
     gene            2201..>3174
                     /locus_tag="VE170_01205"
     CDS             2201..>3174
                     /locus_tag="VE170_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14075.1"
                     /translation="MLKCLTLVTCILFSLTTASAQTPYFQGKTIKIIRGGEPGGTGDM
                     QARAVIPFLKKHIPGNPTIIVENMPGAAGRKAANHIYSTAKPDGLSIGAVGAGLVAGQ
                     ILELAGSQYDIDKLIYLGSTESGDPYVFVGRKDAGLDTLDKLRAVSGIRIGAQAVGHP
                     VYVSGRLFAYLLGLKEPRMVLGYGGPEMDIALEKGEIDVRANGADTVLQRNKDQLEKG
                     FYQFHASITIPKGRYAVGLPKAPELESFAKTDNERQLINLFRTFLYPRWPYLLPPATP
                     KDIAAILRGAMTKALRDPEFHKEFKKLMATDATPLTGEELEAAIKELPR"
BASE COUNT          591 a          964 c          918 g          701 t
ORIGIN      
        1 cgccggatcg ctaaccgcag caatttcgtc acaaggaatc atcgttcatg gacagttctc
       61 tgttcgacgc cgccatcaaa ggcttcgtaa acattctgca cctcaaggtg ttctttgcca
      121 tggccatggg cgttggcatc ggcaccttca ccgccgtcgc gccgcaaggc ctgggcatgc
      181 cgctggttta tgccatcgcc ttgccggtgg tgatcaagtg ggagccggtg accggcatcg
      241 cgtttttgat cggcgccagc tcggtaagcg ccatctgcgc cgcctatctg ccaatcctgt
      301 tcggcattcc cggcggctcg ggctcccaag ccaccgtgct cgatggctat ccgatgggta
      361 aaaaaggcga aggccggcgc gctctcggcg cctccttcat ggccggcggc atgggcgcgt
      421 tgatcggcac attgactctg atggtggcga ttcccgccgc caaaccgtta atttatttaa
      481 tgggttcgcc ggaactgttc gtcatcatgc tgtggggctt gagcatggtc gccgtgctcg
      541 ccggcagccg gccgctcaaa ggactcatcg ccaccgcctt aggcctgatg ctcgccaccg
      601 tcggtcagca agcgcagagc ggcgtaatgc gttttgtcta cgaccaaccc tacctgcttg
      661 acggtatgtc gatcagcatc atggccctgg ctttgttcgg cgtgccttcc gccctcgatc
      721 tcgctttgac caagttgggt gtcgagcagc agccggtgcc gctcaaaggc agtttgttcg
      781 acggcgtcag agatgcattg cgcgagtggt ggttggtgtt gcgctgtagt ttcgtcggcg
      841 tctgggtcgg gatcgttccc ggcttgggtt cgcaggtggt cgactggctc gcctatggcc
      901 atgccgcgca aacctgcaaa ggcggccgag aaactttcgg caccggcgat gtccgcggcg
      961 tgatcgcgcc ggagagcgcc aacgacgcca aggacggcgg cgatttggtg acgaccctgc
     1021 tcttgggctt tccccaaggc gttgtcacgg cgctgttcat cgtcgcgctg ctcgcctggg
     1081 gattcgtccc cggtccggag atgatcaaaa aaaatcccga ggtcatttac agcgtcgtct
     1141 ggcttcaggg aatctccggc atcgtcggca ccctggttgg cttttgcctg gccagccagc
     1201 tcgccaagct cgccgaggtg cgctacacct tcatggttcc gatcatgttc atgttcatcc
     1261 tgatgggtgc cttcagcgtc aaccgcgatc cgctcgatct cgtcgtggtc gtcggcttcg
     1321 gcatcctcgg ttacttcatg cgccgcgccg gctacccgcg cccggcgatg atcctggggc
     1381 tggtgctggg cgatctgatg gaaaaatatc tttaccgctc ggtggcgagc tacggtttta
     1441 gctggatgac ccggccggca gtgatcgtta tttttataat cgctgcggtc tcgctcttct
     1501 ttaccctgcg cggcaaactg aaagcgagca agaaagattc cacggcaacc ttcagcggcg
     1561 ccatcgcgag gtctgacaat gaagactaat ttcgcgctat tcttactcat tatcttcagc
     1621 gtcgccatag cgtccggttg ggggtggccc gacatcgcca agattatgcc ggtctatgtc
     1681 gccgccatcc cgggcctcct aatggtcatg gtgcagattt ttcgcgaggc caccggctgg
     1741 gaaacccgcc gcatcaccac cagcatcgac atggacgaga gcggtcaaaa tcagttggac
     1801 gaagccaccg cacgcaagcg cacgctggcg ttctttgctt ggttcattgc cggcgcattg
     1861 ggaatctggc tgatcggcat cgtctacgct ctgccggtat tggtttttct ctacgccctg
     1921 ctccaaggca aggaaaagat tctcacatcg ctggttatcg gcggctgcgc ctatttactg
     1981 atctggggtt tgtttgaata tatgctcgaa acccgctggc cggccggact actgttcggc
     2041 tattgatact gggggtttaa acgttccagc cgttcaaccg ctgcgctccg ctcaatcccc
     2101 tacctctttc ctcccccgcg acgcggggga ggacgcagga gggggcttgg acgatttgaa
     2161 cggcttgagc gttttaaacc tccttaagga gtagtagtta atgctcaagt gtctaacgct
     2221 tgttacttgc atcctgtttt cgctgaccac cgcgtccgcc cagacccctt actttcaggg
     2281 gaagacgatc aaaataattc gcggcggcga gcccggcggc accggtgaca tgcaagcgcg
     2341 cgcagtcatt cctttcttga aaaaacatat tcccggcaac ccgacgatca tcgtcgaaaa
     2401 catgccgggc gcggcgggac gcaaagccgc caaccacatt tactcgacgg cgaaacccga
     2461 cgggctgtcc atcggcgcgg tgggcgccgg gctggtcgcc ggtcagattc ttgaactggc
     2521 cggctcgcaa tacgatatcg ataaactaat ctatttgggc tccaccgaaa gcggcgatcc
     2581 ctacgtcttc gtcggccgca aggacgccgg tctcgatact ctcgacaaac tgcgcgccgt
     2641 ttcggggatc aggatcggcg cccaagccgt gggccatccg gtctatgtca gcggcagact
     2701 gttcgcctac ctgctcggcc tgaaagaacc gcgaatggtg ctcggctacg gcggtccgga
     2761 aatggacatt gcgctggaga agggagaaat cgatgtgcgg gccaatggcg cggatacggt
     2821 gctgcagcgc aacaaagacc agctcgaaaa aggcttctac caatttcacg cgtcgatcac
     2881 gatacccaag ggacgctatg ccgtcggcct gccgaaagcg ccggagttgg agagctttgc
     2941 caagaccgat aatgagcgcc agctgatcaa tctgtttcgc acttttctct acccgcgctg
     3001 gccctactta ttgccgccgg ccactccaaa agacatcgcc gccatcctgc gcggcgccat
     3061 gacgaaggcg ctacgtgatc ccgagttcca caaagaattt aaaaaactca tggccaccga
     3121 cgccacgccg ctcaccggcg aggaacttga agctgccatc aaagagctgc cgcg
//
LOCUS       DASQKS010000047         6025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5485378, whole genome shotgun sequence.
ACCESSION   DASQKS010000047 DASQKS010000000
VERSION     DASQKS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5485378"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VE170_01210"
     CDS             <1..267
                     /locus_tag="VE170_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYJ14076.1"
                     /translation="FALLTEERKVGATTAVVTSVHLRQIVNEDIPMTEHDIPLNAIVT
                     ADEVIETSSVYARPEGIYWQMLMPEKIAEIPVLQAKRRFRHWDR"
     gene            335..718
                     /gene="hisI"
                     /locus_tag="VE170_01215"
     CDS             335..718
                     /gene="hisI"
                     /locus_tag="VE170_01215"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HYJ14077.1"
                     /translation="MSDVKIDFAKGNGLVPVIVQDHESREVLMVAYMNEEAWQKTQET
                     GKAHYYSRSRKGPWFKGEESGNFQEVKDAYIDCDTDTILIQVRQVGGAACHEGYRSCF
                     FRQRAGADWRVIGERVFDPKAVYKK"
     gene            733..1632
                     /gene="hisG"
                     /locus_tag="VE170_01220"
     CDS             733..1632
                     /gene="hisG"
                     /locus_tag="VE170_01220"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYJ14078.1"
                     /translation="MAGNKELKLGIPKGSLEGMTIELFKKSGWKISSSSRSYFPTIDD
                     SEIRCSLARPQEMARYVEMGTLDVAITGKDWTIECNSDVHVVCEMVYSKTSFRPTRWV
                     LAVPLDSPIKKIEDLKGKRISTEMVEFTKRYFAERNISVDVEFSWGATEAKAAEGLVD
                     AIVEVTETGTTIRAAGLHIIHELMESCPQLIANKTAWQDPWKRKKIEQIKMLLLGALS
                     AENKVGIKLNVPESDLEKVIALIPSITAPTVATLYPSPSLKGIKWFSIESVIDERQVR
                     DLIPQLIQNGAVGIIEYPLNKII"
     gene            complement(1687..3060)
                     /locus_tag="VE170_01225"
     CDS             complement(1687..3060)
                     /locus_tag="VE170_01225"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HYJ14079.1"
                     /translation="MAGKIEIMETVLRDAHQCLVATRMPTKDMLPIAAALDDVGYWSL
                     ETWGGATFDATLRYLKEDPWERLRQLRAAMPKTRFQMLLRGQNVVGYKNYPDDIVQAF
                     VQRAAANGIDVFRIFDAMNDVRNMKTAIDAALKTGKLVEGSVCYTISPVHSPDYFLRV
                     AEKLADMGVQIICLKDMGGLLAPYAAYEIIKKIKSRIPLPLHLHSHCTAGLAQMTYMM
                     AIEAGVDILDAAISPFSQGTSQPATEQLVAALMGTPHDTGLDLAKLAKIADYFYRIRP
                     NYREFESPVNNQIKPDVLISQIPGGMLSNLVAQLRQQKAEDKLEAVLAEMPSVRKDLG
                     YPPLVTPTSQMVGAQAALNVMTGKRYSVVAMETKNYVMGLYGEAPGEIGEEIKAKVLG
                     KKEPITCRPADLLKPGLEKARIDAGSMAKSEEDVLTYALFPDIAKDYFEHRDNLAANP
                     QAAGVSP"
     gene            complement(3086..3631)
                     /locus_tag="VE170_01230"
     CDS             complement(3086..3631)
                     /locus_tag="VE170_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14080.1"
                     /translation="MAEAKKRKGTALLAVMDENCSSCAGSPICEAHCPVDDCINLLYE
                     ELPGGGLKPYRVFVDNDKCIGCQMCYSDDLTKVQQHKETGEIFYEYANRFYDANRKPV
                     PADAVPKRFQLQLIGTESEDRLDKKICPWDAIKMYEFDDGLAKAEYFYDQSKLKQVNG
                     MYVIDAKEKDRLEDKQSDLYD"
     gene            complement(3624..4469)
                     /locus_tag="VE170_01235"
     CDS             complement(3624..4469)
                     /locus_tag="VE170_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ14081.1"
                     /translation="MVHKWLALLSAALAFASALSLSLAAEFTAPFANDQRWEQARKDL
                     AQGRAAEAKAGFDELLRQYPNDADLHLFRGMSLLRLRDPQAAILSIKRAIAINGAHVD
                     ARTLLGYVELEVRGDIPAAINEYRKVIELRPDSADAHSNLAVAQKKHGDLDAAVISLN
                     RALELKPDHVGALTTRGSTFAELGKWSDARRDFEQALKLNPRDDGALYGLSQALREAR
                     EYGGAQSALSQLISRSGNFIYWLEWGRIVMIRYWWALLLIAISWALKDKLRGRFMKAR
                     TQANG"
     gene            complement(4470..4655)
                     /locus_tag="VE170_01240"
     CDS             complement(4470..4655)
                     /locus_tag="VE170_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14082.1"
                     /translation="MEEMSGLHNFLEIVTKPDNIPIVGMLILVVFFTWLGLREALKND
                     KLVEEGREDEIPNEMWK"
     gene            complement(4815..>6025)
                     /locus_tag="VE170_01245"
     CDS             complement(4815..>6025)
                     /locus_tag="VE170_01245"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HYJ14083.1"
                     /translation="IVTGEAVKKKNAEAIAALFSAEAGKFICATAGHNLESILAAYGS
                     GAVHMTYHEGGEFTVMNVDIGGGTSKVAIVKEGRVVDTCAVEVGARLVAMDENGVITR
                     LEDTALKFAKMAGIDGLKLGATMSEEQKEKFSQKLADSLFEVLERGKLSPEVANLLLT
                     PNIEYKEPIHAVMFSGGVSEFVYGYEKRNLGDLGAYLGKKVRAKANQLAGGKIPLRPA
                     EVRIRATVIGASQYTVQVSGNTIYLSHPEILPLRNLQVVTPIFEQKETITVTEVKEAV
                     ERALQRFDADDIDRSVALAIRWELGPSYPLIRTLAEGLVQAMKGHIDNGQPLVLVFDA
                     DIAKLMGNIIERELIPGAGIISIDGIDLKDFDFIDIGQELVDAKAVPVVIKSLIFRNT
                     EHGRGHHGQH"
BASE COUNT         1278 a         1754 c         1675 g         1318 t
ORIGIN      
        1 ttcgccctgt tgacggagga aagaaaggtc ggcgccacca cggccgtcgt cacctcggta
       61 catctccggc aaatcgtcaa cgaagatatc ccgatgactg agcatgatat cccactcaac
      121 gccatcgtta cggccgatga agtgattgag acgagttcag tctatgcacg gccggaggga
      181 atctattggc agatgttaat gccggaaaaa atcgctgaga ttcccgtgct ccaagcaaaa
      241 cgacgctttc gtcattggga tcgctgaaac gtcacgacac catgttgaag ccccccacca
      301 tctatgttag ctaaacagtt tcggagaaaa tcctatgtcg gacgtaaaaa tcgattttgc
      361 caagggtaac ggcctggtac cggtgatcgt gcaggaccac gaaagccgcg aagtgctgat
      421 ggtcgcttac atgaacgagg aagcatggca aaaaactcag gagaccggca aggcccatta
      481 ctacagccgc tcgcgcaaag gcccatggtt caagggtgaa gagtcgggca actttcaaga
      541 ggtcaaagac gcttatatcg attgcgacac cgacacgatc ttgattcaag tgcgccaggt
      601 cggcggcgcc gcctgccatg agggctatag gagctgcttt ttccgccagc gcgcgggcgc
      661 cgactggcgc gtcatcggcg aacgcgtctt cgatcccaag gccgtttata aaaagtaaac
      721 caaggcttcg tcatggcagg caacaaagaa ctcaaactgg gcatccccaa aggcagcctc
      781 gaaggcatga ccatcgagct gttcaaaaaa tccggttgga agatttcgtc gtcgagcagg
      841 agttactttc cgacgatcga tgactcggag atccgttgca gcctggcgcg cccgcaggaa
      901 atggcgcgct acgtcgagat gggcaccctc gacgtcgcca tcaccggcaa ggactggacc
      961 atcgaatgca actccgacgt ccacgtcgtc tgcgagatgg tttattcaaa aaccagtttt
     1021 cgccccaccc gttgggtctt ggcggtaccg ctcgactcgc cgatcaagaa aatcgaagac
     1081 ctcaaaggca agcgcatttc caccgagatg gtggagttca ccaagcgcta ttttgccgag
     1141 cgaaatattt ccgtcgatgt cgagttttcc tggggcgcca ccgaagcgaa agccgccgag
     1201 ggactggtcg acgcgatcgt cgaagtcacc gaaaccggta cgaccatccg cgccgcgggg
     1261 ctgcacatca ttcatgagtt gatggagtcc tgtccgcagc tgatcgccaa caagaccgcc
     1321 tggcaggatc catggaagcg gaaaaagatc gagcagatca agatgctgtt actcggcgcg
     1381 ctgtccgccg agaacaaggt cggcatcaaa ctcaatgtcc cggaaagcga cttggaaaaa
     1441 gtcatcgcgc tgattcccag catcaccgcg ccgacggtgg cgacgcttta cccatcgccg
     1501 agcctcaaag gcatcaagtg gttttccatc gaaagcgtca tcgacgaacg ccaagtgcgc
     1561 gatctgattc cccagctgat acagaacggc gccgtgggaa ttatcgaata tccgctcaac
     1621 aagattatct aaaaattgcc gcgcggttgt cccgcgcagg ctagtcattc cgccgccgcg
     1681 ggtttactag gggctgacgc cggctgcctg tgggtttgct gcaaggttgt cccggtgctc
     1741 gaagtaatcc ttggcaatgt cgggaaacag cgcataggtg agcacgtctt cttcgctctt
     1801 ggccatgctg ccggcatcta tgcgggcttt ttccagtccg ggtttcagta aatccgccgg
     1861 acgacaagtg atcggctctt ttttgccgag aactttcgct ttgatctctt cgccgatctc
     1921 gcccggggct tcgccgtaga gtcccatgac gtagtttttc gtctccattg ccaccacgga
     1981 gtagcgcttg cccgtcatga cgttgagcgc cgcttgcgcg ccgaccatct ggctggtcgg
     2041 cgttaccagc ggtgggtaac ccaaatcctt gcgcaccgag ggcatctcgg cgagcaccgc
     2101 ctccagcttg tcctcggcct tttgctggcg caactgggcg accagattgg atagcatgcc
     2161 gccggggatc tgcgagatca gcacatcggg cttgatttgg ttgtttaccg ggctttcgaa
     2221 ctcgcggtaa ttcggccgga tgcggtaaaa atagtcggcg atcttggcga gcttggcaag
     2281 atccaacccg gtgtcatggg gcgtgcccat gagtgcggcg acgagctgtt ccgtcgccgg
     2341 ctgcgacgtg ccttgggaaa atggcgaaat cgccgcatcg agaatatcca caccggcttc
     2401 gatcgccatc atgtaggtca tctgcgccaa accggccgtg caatgcgagt gcaaatgcag
     2461 cggcagcggg atgcgcgatt tgattttttt aatgatctcg taggcggcgt agggcgcgag
     2521 cagcccgccc atatccttca agcaaatgat ctgcacgccc atgtcggcga gcttttccgc
     2581 cacacggagg aagtaatccg gcgaatgcac cgggctaatg gtgtaacaga ccgagccttc
     2641 gaccagcttg ccggtcttaa gcgccgcgtc aatggcggtc ttcatgttgc gcacatcgtt
     2701 catggcatca aagatgcgga acacgtcgat gccgttggcg gcggcgcgtt gcacgaaggc
     2761 ctgaacgata tcgtcgggat agttcttgta gccgaccacg ttctgtccgc gcaagagcat
     2821 ctggaagcgc gtcttaggca tcgccgcgcg cagttggcgc aagcgctccc agggatcttc
     2881 tttgagatag cgcaatgtcg catcgaaggt cgcgccgccc caggtctcca gcgaccaata
     2941 gccgacgtcg tcaagtgccg ccgcgatcgg cagcatatct ttggtcggca tgcgcgtcgc
     3001 caccaagcac tggtgcgcgt cgcgcagcac ggtctccatg atttcgattt tacccgccat
     3061 atgcgtcggc tccctgttgg gctgctcaat cgtagagatc gctctgtttg tcttccaaac
     3121 gatccttctc tttggcatcg atgacgtaca tgccgttgac ctgcttcaac ttgctctggt
     3181 cgtaaaagta ctccgccttg gccaaaccgt cgtcgaactc gtacatcttg atggcgtccc
     3241 agggacagat tttcttgtcc agacggtctt ccgactcggt gccgatgagt tgcagttgaa
     3301 agcgcttggg cacggcatcg gctgggacgg gcttgcgatt ggcgtcgtaa aaacggttgg
     3361 cgtattcgta aaaaatctcg ccggtttcct tatgctgctg cactttggtc agatcatcgc
     3421 tatagcacat ttggcagccg atgcacttat cattgtcgac gaaaacccgg tagggcttca
     3481 aaccgcctcc cggcagctcc tcatagagca gattgatgca gtcatccacc ggacagtggg
     3541 cttcgcagat gggcgagccg gcacagctcg aacagttctc gtccatcacc gccaatagcg
     3601 ccgtaccttt gcgctttttt gcctcagcca ttcgcttgcg tcctcgcttt catgaacctg
     3661 ccgcgtaatt tgtccttcag cgcccagcta atcgcgatca gcaacagcgc ccaccaataa
     3721 cgaatcatca cgatgcggcc ccattcgagc caataaatga aatttcccga gcgcgaaatc
     3781 aattggctca aggcgctctg agcgccgccg tactccctgg cttcgcgcaa cgcttgcgac
     3841 agaccataga gcgcgccgtc gtcgcgcgga ttgagcttga gcgcctgctc gaaatcgcgc
     3901 cgcgcgtcgc tccacttgcc gagctcggcg aaagtgctgc cgcgggtcgt caacgccccg
     3961 acatggtcgg gcttgagttc cagcgctcgg ttcaaactga tcaccgcggc gtccaaatcc
     4021 ccgtgctttt tctgtgccac cgccagattg ctatgggcgt ccgcggagtc gggccgcagt
     4081 tcgatgactt tgcggtactc attgatcgcc gctggaatat cgccgcggac ttctaactcg
     4141 acatagccca gcagtgtgcg ggcgtcgaca tgggccccgt taatggcgat cgcccgcttg
     4201 atcgacaaga tcgccgcctg ggggtcgcgc aaccgcaata gcgacatgcc ccggaacaga
     4261 tgcaagtcgg cgtcattggg atattgcctg aggagctcat cgaacccggc tttagcttca
     4321 gcggctctac cctgggcgag gtccttacgc gcctgttccc agcgttgatc gttagcgaaa
     4381 ggagcggtga attcggcggc gagggagaga ctaagcgctg aagcgaatgc cagggcggcg
     4441 ctcagcagcg ccagccactt gtgcaccatt tatttccaca tttcgttagg gatctcatct
     4501 tctctgcctt cttcaaccag cttgtcgttt ttcaatgctt cccgcaagcc gagccaggta
     4561 aagaaaacca ccaagatcaa catcccaacg atcggaatat tgtcgggctt ggtgacgatc
     4621 tcgagaaaat tgtgcagtcc gctcatttct tccataaaag ttaaacactc gttttttaag
     4681 gattttaggg aaaaatatta gaaatcccgg aggaagtcaa tgtaaccact gacggtgtct
     4741 gcgcggtctt gaagtagcga cgatgaagaa tcgtaaagac tgccgaatcc gccatcatcc
     4801 atcgacgaat tttactagtg ttgcccatga tggccgcggc catgctcggt gttgcggaaa
     4861 atcaacgact tgatcaccac cggcaccgct ttggcatcga ccagctcttg accgatgtcg
     4921 atgaaatcga aatcctttag atcgatgccg tcgatggaaa taatccccgc gccgggaatc
     4981 agctcgcgct cgataatatt gcccattaac ttggcgatat cggcgtcgaa gactaatacc
     5041 agcggctggc cattgtcgat atggcccttc atggcctgga ccaagccctc ggccaaagtg
     5101 cgaatcaatg gataggacgg tcccagttcc cagcggatcg ccagggccac cgaacggtcg
     5161 atgtcatcgg cgtcaaaccg ctgcagcgcc cgctcgacgg cttctttgac ctcggtcaca
     5221 gtgatggttt ctttttgctc gaagatcggt gtgaccactt ggaggttgcg caagggcagg
     5281 atttcaggat gagacaggta gatcgtatta ccgctcacct gcacggtata ttgcgacgcg
     5341 ccgatgaccg tggcgcggat ccgtacttcg gccgggcgca aggggatctt gccgcccgcc
     5401 agttggttcg ccttggcgcg cacctttttg ccaagatagg cgccgaggtc gcctaaattg
     5461 cgcttctcat agccgtagac aaactcggaa acgccgccgg aaaacatcac cgcgtggatc
     5521 ggttccttgt actcgatgtt gggcgtgagc aagaggttgg ctacttcggg cgaaagcttc
     5581 ccgcgctcca acacttcgaa tagcgaatcg gccagcttct gtgagaattt ttctttttgt
     5641 tcttcgctca tggtggcgcc gagcttgagg ccgtcgatgc ccgccatctt ggcgaacttg
     5701 agcgcggtgt cttcaagacg ggtgatcacg ccgttctcat ccatggcgac caaccgggcg
     5761 cccacttcga cggcgcaggt atcgaccacc cggccctctt tgacgatggc aaccttgctc
     5821 gtgccaccgc cgatgtcgac gttcatcacc gtgaactctc caccctcgtg ataggtcata
     5881 tgcaccgcgc cggagccgta ggcggccaag atcgattcca gattgtgacc cgccgtggcg
     5941 caaatgaact ttcccgcctc cgcggaaaac agcgcggcga tggcttcggc gttttttttc
     6001 ttcaccgctt cgccggtgac gatta
//
LOCUS       DASQKS010000048         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5906570, whole genome shotgun sequence.
ACCESSION   DASQKS010000048 DASQKS010000000
VERSION     DASQKS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5906570"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            541..1431
                     /locus_tag="VE170_01250"
     CDS             541..1431
                     /locus_tag="VE170_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14084.1"
                     /translation="MGYLAAVSASADAPRLEAFRQGLRDFGYIEGKNIFVDYRHQSGS
                     FERLPDLAAELIALKIDVLVAVTTNAALAAKKATTTVPIVFMGVTDPITAGLVESLAR
                     PGGNSTGVTNMAAILTGKRLELLKEAIPKVRRVAVLWDPQAPGSTPQWNESQEPARAL
                     GLQLHSMQVSSADKYEAAFKEAVKARSTAVWITLNPLANSNQKLLAALAVSHRLPSIC
                     ARADYAENGCLIAYGPGYTTEGKDGARYVDKILKGVKPHEIPVEQPMKFELLVNLKTA
                     KQIGVTVPPLVLYRADKLLK"
     gene            1607..2830
                     /locus_tag="VE170_01255"
     CDS             1607..2830
                     /locus_tag="VE170_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14085.1"
                     /translation="MHSTGPRKEFRDNHLMAHQRLDTIWTRTFILLCAAQLLSYAQHF
                     MLAPVLPLFLTQLGAPPVVVGIVLACFAVTSVIVRPVVGHWADRWSEAGVMIAGSLFQ
                     AAAIFLCFMPFISATALANGLRGIGWAGLNTGGYSLLALISPEKKRGAASGLYSGVQS
                     SAQIFFPPIALWLLYASFGGYRLVFVITAIFSVAGAIAGWAMASSVPHPAKANRTDET
                     TDWWREIFHFVEPEILLPSLLLLWLNLALPAITNFSILYAKELGIGNFAPYFVVIGAT
                     SMLGRPLLGRLSDRIGRARSIAAGFALQTVALVLITAMTNLLGMIVCGAIYMLGNAIG
                     SSTTLALAVERADPQRRGKQMATFSVAYPLSYGVGSLITGSAIDIAGYIGMFLLLAIT
                     QAGGLLFIVGRRAKL"
     gene            2978..>3938
                     /locus_tag="VE170_01260"
     CDS             2978..>3938
                     /locus_tag="VE170_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14086.1"
                     /translation="MSVASTNRSLWTRGFVLLCVAEFLCYAQHFVLQPALPLYITHLG
                     GSPFIVGLVIAAFGITSVISRPIIGYWVDRWSAPGMMVLGMIGQGLSILFCLLPINAA
                     VIVSNALRGIGWSSMAASGYTLLATSAPPQRRGEASGYFGGVQSSATIIFPAIALWII
                     DSPLGGFRAVFLLAMTFVFAGAIAAWALARIAPRHSQESKMDAGEPWWREIINVVDRH
                     ILTAALLIFTLNMSLPCFSSFIVLYARDLGVSNFGWYYVAVGVTSALGRPLLGRFSDK
                     FGAGRSLLIAFSLETVALLIMPLAGSLPGLILGGALWYTGAAIG"
BASE COUNT          711 a         1200 c         1116 g          911 t
ORIGIN      
        1 ccgggcgcaa ctgaaatgat ctaaggctag tggatttgag catgcggtgc cgccggcgtt
       61 atcccgagac ctgccagttg catcgcccgc cgcaagcgcg tcgatactca ccctcgtaac
      121 cgccgttgaa ataagttatg aaaatcaaaa cacttgcgcg ctgcacgttg acgtgtcgca
      181 aacactttcg ctattacttt ctcagaggtt ggccgccggc gtttgcctgc tggacctgcc
      241 gtggcccgcg gcattcctag cggcagagcc gccaacgcag gaaagaagta gtctaagccc
      301 atgagaatac tattgcgtta accacttcat cgtctcgtcg aaagatcgcc ggggcgcggc
      361 agacgcggca agtttacttt ccaggctttc tcgatcatcc agcttgaaaa gttcttggat
      421 tggccgtcag gataactcag aaatccgatg agtagaaaaa ctctggccgc ttgtctttgc
      481 gctctactct tgaccgcaag cgttccggtc caggcacagc agccaaagag gatcccgcga
      541 gtggggtatc tcgcggccgt ttctgcttcc gctgacgcgc ctcggctgga ggcgtttcgc
      601 caagggctgc gtgactttgg ctatattgag ggcaagaaca tttttgttga ctaccgacat
      661 caaagcggca gcttcgagcg ccttcccgac cttgccgccg aattgatcgc tctgaaaatc
      721 gacgtgctcg tcgcagtcac gacgaatgcg gctctcgctg ccaagaaggc taccacgacc
      781 gttcccatcg tttttatggg agtgaccgat ccgattaccg ctgggttggt cgagagtctg
      841 gcgcgacccg gcggaaacag tactggggtt accaacatgg cggcgatttt gaccggtaaa
      901 cgactggagt tattgaagga ggccattccc aaggtgcgtc gtgttgccgt cctgtgggac
      961 cctcaggccc cgggttcgac accacaatgg aacgaaagcc aggagccggc ccgggcgctg
     1021 ggcctgcaac ttcattcaat gcaggtaagc agcgccgaca aatacgaggc cgcttttaaa
     1081 gaagccgtca aggcacgcag caccgctgtc tggatcacac tgaacccttt ggccaattct
     1141 aaccaaaaat tgctcgcggc gctggccgtc agtcatcgct tgccatcgat atgcgcgcgc
     1201 gcagattacg cggaaaatgg ttgtctaatc gcctatggac ccggttacac taccgagggt
     1261 aaagacggag cgcgctacgt cgacaagatc ttgaagggcg tgaagcctca cgagattcca
     1321 gtggagcagc cgatgaaatt cgagctcctg gttaatttga agaccgccaa gcagatcggc
     1381 gtcacggttc cgcccttggt gctttatcgg gccgataagc tgctgaagta acaaagcgat
     1441 cggatattgc tcaccgcctg gttttgccca tcagccaggc gcagacgcga gcggctgaag
     1501 agcgtcgcag atcggttacc agcctcgctt ccgtagtttt tccatcaact cgattccaat
     1561 ctcggttcgt gttagcgtgg gcgcgctttg ccgcctacgt gtcgtcttgc attcgacagg
     1621 cccgcgcaaa gaatttcgcg acaatcattt gatggcacac cagcggctcg ataccatctg
     1681 gacgcgcacg tttattcttt tatgtgccgc tcagttgctt agctacgcgc agcatttcat
     1741 gctcgcgccg gtgctaccgc ttttcctcac ccaactggga gcgccgccgg tcgtcgtcgg
     1801 catcgtgctc gcctgcttcg ccgtcaccag cgtcatcgtc cgtccggtgg tcggtcactg
     1861 ggccgaccgc tggagcgaag ccggcgtaat gatcgccggc tcgctgtttc aagcggcggc
     1921 tatttttctc tgcttcatgc cgtttatctc cgccacggca ttggccaacg gcctgcgcgg
     1981 catcggctgg gccggattga acaccggcgg ctactcgctc ctagcgttga tctcgccgga
     2041 gaaaaaacgc ggcgccgcct cgggtctcta cagcggcgtg caaagctcgg cgcaaatctt
     2101 ttttccgccc atcgccctct ggctgctcta cgcctccttc ggcggctacc gcctggtatt
     2161 cgtcatcacc gcgatctttt ccgtcgccgg agcaatcgcc ggctgggcga tggcgtcgtc
     2221 cgtgccgcat ccggcgaagg cgaatcgcac cgacgagaca accgattggt ggcgcgaaat
     2281 atttcatttc gtcgaaccgg agatcttact gccgtcgctg cttttgctct ggctcaacct
     2341 ggcgctgccg gcgatcacca acttcagcat tctctacgcc aaagaactcg gcatcggcaa
     2401 tttcgcgccc tacttcgttg tcatcggcgc caccagcatg ctcggccggc cgctgctcgg
     2461 acgcctgtcc gatcgcattg gccgcgctcg ttccatcgcc gccggattcg cgctgcagac
     2521 ggttgctctg gtgctcatta ccgccatgac gaatttactg ggtatgattg tctgcggggc
     2581 gatttacatg ctcggcaacg ccatcggcag ctcgacgacg cttgcgctgg ccgtcgaacg
     2641 cgccgatccc caacgccggg gtaaacagat ggcgactttc tccgtcgctt atccgttaag
     2701 ctatggcgtc ggctcgctca tcaccggcag cgccatcgac atcgccggct acatcggcat
     2761 gttcttactg ctggcaatca cgcaagcagg cggactgtta ttcatcgtcg ggcgacgcgc
     2821 caaactgtag agaatcggag tgatggagta ttggaatggt ggaatgaagg agcggtgggc
     2881 gttgcgaacc cccaatactc catactccaa cactccatca ctccaatctt ccccgccgcc
     2941 gcatgagttt ttgcaggcgc catgctagac cacaacgatg tccgttgcat cgacgaaccg
     3001 atccctctgg acgcgagggt ttgtgctgct ctgcgtggcg gaatttcttt gctatgcgca
     3061 gcacttcgtt ttgcagccgg cgctgcccct ttatatcacc cacctgggtg gctcgccgtt
     3121 catcgtcggt ctggttatcg ccgctttcgg cattaccagc gtcatctcgc gaccgatcat
     3181 cggctattgg gtcgatcgct ggagcgctcc cgggatgatg gttctcggca tgatcggcca
     3241 ggggctgagc attttgtttt gcttgctccc gatcaacgcc gccgtgatcg tttccaacgc
     3301 gttgcgcggt attggctggt cgagcatggc ggcatcgggt tacacgttgc tggcgacgtc
     3361 cgcaccgccg caacgtcggg gagaagcatc cggctatttc ggcggcgtcc aatccagcgc
     3421 aacgattatt tttccggcca ttgcgctttg gatcatcgac tcacccctgg gcggtttccg
     3481 cgcggtattt ctgttggcca tgactttcgt tttcgccggc gcaatcgccg catgggctct
     3541 ggcccgtatc gcaccgcggc acagccagga aagtaaaatg gacgccgggg aaccttggtg
     3601 gcgcgagatt atcaatgtcg tcgaccgcca cattctcacc gcggcgttgc tgatttttac
     3661 tttgaacatg tcgctgccct gcttctccag cttcattgtg ctttacgccc gcgatctcgg
     3721 cgtcagtaac ttcgggtggt attacgttgc cgtcggcgtg accagtgcgt tggggcgccc
     3781 gctgcttggc cgtttttccg acaagttcgg cgccggccgg tcattgctga tcgctttcag
     3841 tctagagact gtcgccttgc tgatcatgcc gctcgccggc agcctcccgg gcttgatctt
     3901 gggcggtgct ctttggtaca cgggcgcggc catcggcg
//
LOCUS       DASQKS010000049         7882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2965836, whole genome shotgun sequence.
ACCESSION   DASQKS010000049 DASQKS010000000
VERSION     DASQKS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965836"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VE170_01265"
     CDS             complement(<1..1099)
                     /locus_tag="VE170_01265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ14087.1"
                     /translation="MDFNLTMEQQQIREEIKKVCQEFPDAYWRKVDSEKAYPDAFVKK
                     LGDLGWLAALIPEEFGGTGLGITEASIILEEINRSGGVATACHAQMYTMGTLLRHGNA
                     EQKQRYLPKIATGELRLQAFAVTEPNAGSEATRLQTSAVKKGDRYLINGEKIFISRVL
                     QSDLMLLLARTTPYDELPDKTRGLSVFIVDLTANQGKLEVKPLDLMINHHTNALFFDN
                     VEVPVENLIGDEGMGFRYIIDGWNAERILVAAEAVGDGRWFVERAAKYASERNVFGKP
                     IGANQGVQFPIAKAYANIEAADLVRYQAATKFDRKEKCGAEANLAKYLASEAAWEAAN
                     ACLTTHGGYGFAAEYDVERKFRETRLLTVAPV"
     gene            1164..1673
                     /locus_tag="VE170_01270"
     CDS             1164..1673
                     /locus_tag="VE170_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HYJ14088.1"
                     /translation="MAVKPGWEGRYFEDFEIGDIYRCRLGRTVTEADNIWFTLLTNNT
                     NQIHFNNEYGKRTEFGKCLINSALTLAIVAGMGVADVSENGFALGWDGITLPNPLFAG
                     DTLYSESEVLETRESKSKPQWGILKVRTRGVQQEGKVVIDYARSVMVWKKAFAPKNDL
                     FPQIKEEKK"
     gene            1749..2948
                     /locus_tag="VE170_01275"
     CDS             1749..2948
                     /locus_tag="VE170_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYJ14089.1"
                     /translation="MASNSKPSRALDGVKILAFEQVLSGPFATCLLADMGAEVIKVER
                     PGVGDVIRSWDSVVKGLSSGYVWLNRNKRSLTVDVKKEKGREILQGLAKRSDIFFENY
                     APGVAGRLGLGYEKLSDLNRRLIYCSLSGYGQDGPYRDVKAYDLLIQGEGGIIATTGY
                     PDKPARAGIAIADIASGMYAAIGILLALYQREKTGEGQLVDVSMLDSIVSWLGYFPHH
                     YWHAGEEPGRVGMRHHYVCPYGPYLAGDGEYVNLAVASPADWDVFCKVVIEKPEMLTD
                     ARFASVQDRRKNRAVLEETIEEIFAARDHRHWLAQLKKADLPHGIVRGIAQVLAHPQV
                     AARKLIREADSPVGKVPVIANALKMSKSDARYERIPGLGENNAAILAELGYDAAAIEA
                     FRRDKII"
     gene            3066..3638
                     /locus_tag="VE170_01280"
     CDS             3066..3638
                     /locus_tag="VE170_01280"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14090.1"
                     /translation="MRKPVVGVMGASANDGLSLAESERLRQLAESLGTAIAQADCFLV
                     TGATTGLPGMVARAFRAQGGFALGISPAENRAEHVQRYNLPEDGADVIVYTGFGYKGR
                     NVINIRSSDIVIIFGGATGTLNEFTIAYDEGKIIGVLEGSGGVADHIKSIVAACQKPS
                     RGKLFFDSSPKALIQTCMDAIAALDHLGNR"
     gene            complement(3620..4183)
                     /locus_tag="VE170_01285"
     CDS             complement(3620..4183)
                     /locus_tag="VE170_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14091.1"
                     /translation="MKIYFIGALVASLAAGAPVETPPRRSELPPFGQLDRKTKVNVPV
                     ILCIDDKPTVGGQPSGEAYANAAANGFRSVMTLRSPQDGVNTLRERLLVENHRMRYFN
                     IPTSNPWPSVKQIDEFLKTARDPANHPMLINCAFAERVAPYMLIFRMQDEGWTEEKAL
                     EETVQLGLPRDDMRKFARGYLSQRLPK"
     gene            complement(4180..5178)
                     /locus_tag="VE170_01290"
     CDS             complement(4180..5178)
                     /locus_tag="VE170_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14092.1"
                     /translation="MASAELIVDVHHHYMPALLFDRLAKQAGGKRIVTNEISLTLHPS
                     RKDLEAHIKAMDDSGVTYSILTDQVQVMGAEVARALNDGVAEVEKNHPKRFRSAIHMA
                     IHEPESAQRELERGIHELGLRSVALLACHLDIQLDNPLMFPLYEIIQKNNLPIILHPQ
                     SKPTGSETTYSLDRCVFRPLETTQAIVRIMNAVLPRFPELRFIIPHLGGAASSLKGRM
                     MAFFETDDAPIPAEMRGYLKTQNEQKKFGVTERFEKLFQRLYFDTAGTGAWGPSMAAA
                     LNITTADRIMFGSDYPLECKTAANIVESIDMVRQAPCSSADKTAILGNTAAGVFNL"
     gene            complement(5298..6002)
                     /locus_tag="VE170_01295"
     CDS             complement(5298..6002)
                     /locus_tag="VE170_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14093.1"
                     /translation="MAAYLNRYFSFLLILTLLAPLWLSVFNTSEPQPAGALHTALIWS
                     GLLMGGWSVLRFVRRYHVRGIWRSPTRQESSIYVRLLLAVLLIDFGSKALFFRWDRPE
                     QVEILPNFGLHSVFHATEFETFHIYLLLYFSYWFLLGPWYFRFSNPTLDRLWLGSTPF
                     ALAGALALSFERLLFGGVHNSFYFAGPLMWLCPPCASPAFKSYAWTPADFFVHAAILP
                     IIILLLSYYPTTSRRI"
     gene            complement(5987..6682)
                     /locus_tag="VE170_01300"
     CDS             complement(5987..6682)
                     /locus_tag="VE170_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYJ14094.1"
                     /translation="MITTVIFDLDGLLADTEELHCRAYRMALLEDNIELAEAEYCDHW
                     VRLGKGIDDWVAHQGLELDPAALRARKAAHYQTLLQSSLRPMTGATALLARLQGNFRI
                     GLASSSYRDAVDGVLLGLGIADCFETVVSGSDVSRVKPAPEIFLKAAERLGVVPTECV
                     VLEDAEKGVLAAQAAGMRCIAIPNGYTRGHDFSRATLVCGSLHEVTVELLRTLGTNSK
                     PVFRTATAPWPHT"
     gene            complement(6815..7318)
                     /locus_tag="VE170_01305"
     CDS             complement(6815..7318)
                     /locus_tag="VE170_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14095.1"
                     /translation="MNKVSSLIFGGAALATVALVSAPMAAAPEGGGRRVERGAHMAGG
                     FAGGAPLISIALKHKSELSLSNDQVAKLEQIKSNFQTQVTPLHQQMRTIEKEIAGLRE
                     QSPADLVKIKSKIQETEKYRSELRYLQYEALDHGQAVLSVQQRDQLKTLVAARHENFR
                     KMPRQQS"
     gene            7653..>7882
                     /locus_tag="VE170_01310"
     CDS             7653..>7882
                     /locus_tag="VE170_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14096.1"
                     /translation="MENQVVGGNLSGPRLAYLHHVSLPCRDLAESKRFYIEVLGGELY
                     HDTSGFAEVMLADMIVGMSEQAGGWTGWEAEYP"
BASE COUNT         1684 a         2049 c         2364 g         1785 t
ORIGIN      
        1 tcaccggcgc caccgtcagt aaacgggtct cgcgaaactt tcgctcgacg tcatattcgg
       61 cggcaaagcc gtagcctccg tgggtcgtca ggcaagcgtt ggcggcctcc caggcggctt
      121 ccgacgcgag gtatttggcg agatttgctt ccgcgccaca tttctctttt cggtcaaact
      181 ttgtcgcggc ttgatagcgc accagatcgg cggcttcgat gttcgcgtaa gccttcgcaa
      241 tgggaaactg cacgccttga ttggcgccga tcggcttgcc gaacacatta cgttcgctgg
      301 cgtacttggc ggcgcgctcg acaaaccaac ggccgtcgcc gaccgcttcg gcggcgacga
      361 ggattctttc ggcgttccag ccatcgatga tgtagcgaaa gcccatgcct tcgtcgccga
      421 tgagattttc aaccggcact tcaacattat cgaaaaacaa ggcattggta tgatgattga
      481 tcatcaagtc aaggggctta acttcaagtt ttccttgatt ggctgtcaag tcgacgatga
      541 acaccgatag gccccgagtt ttgtccggca attcgtcgta gggtgtggtt ctcgccagca
      601 gcagcatcaa gtccgactgc agcacgcgtg agataaatat tttttcgccg ttgatcaaat
      661 agcggtcgcc ctttttaacg gcgctggttt gcaggcgtgt cgcctcagag ccggcgttgg
      721 gttcggtgac ggcaaaggct tgcaggcgca gttcgccggt ggcgattttc ggcagatagc
      781 gctgtttttg ctcggcgttg ccgtgacgca agagcgtgcc catggtgtac atttgcgcgt
      841 ggcaggcggt ggcgacgccg ccggagcggt tgatctcttc caagatgatg ctcgcttcgg
      901 tgatgccgag gccggtgccg ccaaattctt ccggaatgag tgcggccagc cagccgaggt
      961 caccgagttt ttttacaaaa gcatcggggt aggctttttc gctgtcgacc ttgcgccagt
     1021 aggcatcggg aaattcttga cagacttttt tgatctcttc gcgaatctgt tgctgctcca
     1081 tcgtcaggtt gaaatccatc gatgcctcct atagttcgta gactataacc taatgttttt
     1141 agtgcctaaa gaggaggaac gtcatggccg tcaagcccgg ctgggaaggc cgctattttg
     1201 aagacttcga gatcggcgat atttatcgtt gccgtctcgg tcgcaccgtc accgaagccg
     1261 ataatatctg gtttaccttg ctgaccaaca ataccaacca gattcacttc aacaacgagt
     1321 atggtaaacg cactgagttc ggcaaatgtc tgatcaacag cgccttgacc ctcgctatcg
     1381 tcgccggcat gggtgtggcc gatgtcagtg aaaacggttt tgccctggga tgggatggga
     1441 tcaccttacc gaatccttta tttgccggag atacgctgta ttccgaatcc gaagtgttgg
     1501 agacgcgtga gtccaagtcc aaaccgcagt ggggaattct caaagtgcgc actcgcggtg
     1561 tgcaacaaga gggcaaggtt gtcatcgact acgcgcgcag cgtgatggtg tggaagaaag
     1621 ccttcgcgcc gaagaacgat ttgtttccgc agatcaaaga agagaagaaa taattgccgt
     1681 catgacgatc aagatgttca aaacgttcag gccgtttgag accaaatgaa acaagttaag
     1741 aggtgagtgt ggcaagcaat tcgaaaccat cgcgagcgct ggatggggtc aaaattttgg
     1801 ctttcgagca ggtgctctcc ggcccgtttg cgacctgctt gctcgctgac atgggcgcgg
     1861 aggtgatcaa agtagagcgc cctggtgtcg gcgatgtgat tcgctcctgg gattcggtgg
     1921 tcaaggggct gtcgtcgggt tatgtttggt tgaatcgcaa caagcgcagc ttgacggtcg
     1981 acgttaagaa ggaaaagggt cgagagattt tacaagggtt ggccaagcgc tcggatattt
     2041 tcttcgagaa ctatgctccg ggtgtcgcgg gacggcttgg gctcggctat gaaaaacttt
     2101 cggatctcaa ccggcggctg atctattgtt cgctttccgg ttacggtcaa gacggtcctt
     2161 atcgcgatgt caaggcatac gatctcttga ttcaaggcga gggcggcatc atcgccacca
     2221 ccgggtatcc cgacaagccg gcgcgcgccg gcatcgccat cgccgacatc gcttccggca
     2281 tgtatgccgc aattggtatt ctcctggcgc tctaccagcg ggaaaagacc ggtgaagggc
     2341 agctcgtcga tgtgtcgatg ttggattcga tcgtctcatg gctcggctac tttccacatc
     2401 attattggca tgccggcgaa gagccggggc gagtgggcat gcgtcaccac tatgtttgtc
     2461 cctacggccc ttacttggcc ggtgatggtg agtatgtcaa tctggcggtc gcgagtccgg
     2521 cggattggga tgtgttttgc aaagtcgtta tcgaaaagcc ggagatgctt acggatgcgc
     2581 gcttcgcatc ggtacaggat cggcgcaaga atcgcgccgt gctggaagaa accatcgagg
     2641 aaatttttgc cgcgcgggac caccggcact ggctggcgca attgaaaaaa gccgatctgc
     2701 cccatggcat cgtccgcggc atcgcccagg tgctcgccca tccccaggta gcggcgcgca
     2761 agctgatacg cgaagccgac tcgccggtgg gcaaggttcc ggtgattgcc aacgccctca
     2821 agatgtcgaa gagcgatgcg cgttacgagc gtattccggg tctgggtgag aacaatgcgg
     2881 cgatcttggc ggaacttggt tacgatgccg ctgcgatcga agcgttccgg cgcgataaaa
     2941 tcatttgatc gacgcgggtt tcaccaacaa ccgatcgaac actttgcaat ctagccgctg
     3001 gcggcatcga tctgcgccgt gccgtaatac cgtgctgact tacgactccg cttcggagac
     3061 tggctatgcg gaaacctgtt gtcggtgtca tgggtgcgtc ggccaacgac ggcctcagtc
     3121 tcgcagagtc ggagcgactc cggcaacttg ctgaaagctt gggaacggcg atcgcccaag
     3181 ccgactgttt cctggtcacc ggcgcgacca ctgggctacc tgggatggtc gcgcgggcgt
     3241 ttcgcgcgca gggtgggttt gccttgggga tttccccggc cgagaaccgc gccgagcatg
     3301 tgcagcgcta taatttaccg gaagatggcg cggatgtgat cgtctatacc gggttcggtt
     3361 acaaaggtcg taacgtcatc aatatccgct cgtcggatat tgtcatcatt ttcggcggcg
     3421 ccaccggaac gctgaatgaa ttcacgatcg cttacgatga aggcaagatt atcggcgttc
     3481 tggaaggcag cggcggggtg gcggatcata tcaagagtat cgtcgcagct tgtcagaaac
     3541 ctagccgggg caagctgttc ttcgattcgt ctcctaaagc gcttattcag acttgcatgg
     3601 acgcgatcgc tgcattggat cacttgggta accgctgact caaatagccg cgggcaaatt
     3661 tgcgcatgtc gtcgcgcggc agtcccaatt gcacggtttc ttctaatgcc ttttcttcag
     3721 tccagccttc gtcctgcatg cggaagatca gcatgtaggg ggcgactcgc tcggcgaagg
     3781 cgcagttgat caacatcgga tggttggcgg gatctctcgc ggttttgaga aattcgtcga
     3841 tctgcttgac gctgggccat gggtttgagg tcgggatatt aaagtaacgc atccggtgat
     3901 tttcgaccaa aagacgttca cgcaatgtgt tgacaccatc ttgcggcgat cgcagggtca
     3961 tgaccgagcg aaagccattc gccgcggcat tggcgtaggc ttcgcccgaa ggttggccgc
     4021 cgacggtggg tttatcgtcg atgcagagga taaccggaac gttgactttg gttttgcgat
     4081 cgagctggcc gaatggcggc agttcactgc gacgcggggg ggtctccacc ggcgcgccag
     4141 ccgccagcga tgcgaccagc gcgccgatga aataaatctt cataggttga aaacgccggc
     4201 cgcggtattg ccgaggatcg cggttttgtc cgcgctcgag cagggcgctt ggcgtaccat
     4261 atcgatcgac tcgacgatgt tcgccgcggt tttacactcg agcggatagt cgctgccaaa
     4321 catgattcgg tcggcggtgg taatgtttaa agccgcggcc atcgacggtc cccaggcgcc
     4381 ggttcccgcg gtgtcgaagt acagccgttg aaagagcttt tcaaaccgtt ctgtgactcc
     4441 gaattttttc tgttcatttt gggttttcaa atagccgcgc atttccgccg gaatcggcgc
     4501 gtcatcagtt tcgaaaaacg ccatcatgcg tcccttgagc gacgacgccg cgccgccgag
     4561 gtggggaatg atgaagcgga gctcgggaaa gcgcggcagg acggcattca tgatgcgcac
     4621 gatcgcttgg gtggtctcga gcggacgaaa cacgcaacgg tccaaactgt aggtcgtttc
     4681 cgagccagtc ggtttggatt gcggatggag gatgatcggc aaattattct tctggatgat
     4741 ctcatagagc ggaaacatca gcggattgtc gagctggata tcgaggtggc aggcgagcag
     4801 ggcgacggag cgcaggccga gctcatgaat gccgcgttcg agctcgcgct gcgcgctctc
     4861 gggctcatga attgccatat ggatggcgct gcgaaaacgt ttcgggtggt ttttttcaac
     4921 ctcggcgacg ccgtcgttga gagcgcgcgc gacttcggca cccatgacct gcacttggtc
     4981 ggtgagaatg gagtaggtca ctccactgtc gtccatcgct ttgatatggg cttcgaggtc
     5041 cttgcgcgaa ggatgcagag ttaaactgat ttcgttggtg acgatgcgtt tgccgcccgc
     5101 ctgtttggcg agacggtcga atagcaaagc cggcatataa tggtggtgca cgtcgacaat
     5161 caattcggct gaggccataa taaatttcca actccttgtc tagtattctt ggtgcatcaa
     5221 actacgatag gccggtggtg actgcaagtg aactctgcgg cgcagtgatc gaggacggat
     5281 gtatattcaa tgaagggtta aattctccga ctggtagttg gataatagga cagcaacagg
     5341 atgataatcg gcaagatggc ggcgtggacg aaaaaatccg ctggagtcca ggcgtagctt
     5401 ttgaacgccg gcgatgcgca gggcgggcaa agccacatga gtggcccggc gaagtaaaat
     5461 gaattgtgca cgccgccgaa gagaaggcgc tcgaagctca gtgccagggc gccggccagc
     5521 gcgaaagggg tcgaaccaag ccagagccgg tccagagtgg gattggaaaa gcgaaaatac
     5581 cacggtccga gcaaaaacca ataagagaaa tagagcagga gataaatgtg aaaggtttca
     5641 aactcggttg cgtgaaacac cgagtgcaat ccaaaattcg gcaatatctc gacttgttcc
     5701 ggccgatccc agcgaaaaaa taacgctttg gacccgaaat cgatgagcag cacggccaac
     5761 aacaggcgaa cataaattga cgattcctgc cgcgttgggc tgcgccaaat gccgcgcaca
     5821 tggtagcgcc tgacgaagcg gagaactgac cagccgccca tgagcaggcc gctccaaatc
     5881 agcgcggtat gaagcgcccc ggcgggttgc ggctctgacg tattaaagac gctgagccaa
     5941 agcggcgcga gcaacgttaa tatcagcagg aaagaaaagt atcgattcag gtatgcggcc
     6001 atggcgcggt agctgtccga aatactggtt tggaatttgt cccaagggtg cgtaacagtt
     6061 cgaccgtaac ttcatgcagc gagccgcata caagcgtagc tcgggaaaaa tcatgacctc
     6121 gggtgtagcc gttggggatg gcaatgcagc gcatgccggc ggcttgggcc gccagcacgc
     6181 ctttctcggc gtcttcgagg acgacgcact cggttggaac gacgccaagg cgctcggcgg
     6241 ctttgagaaa aatctccggc gccggtttaa cccgggagac gtccgagccg ctgacgacgg
     6301 tttcaaaaca gtcggcgatg cccaagccaa gaaggacgcc gtcaacggca tccctgtatg
     6361 aagaagaggc gagaccgata cgaaaatttc cctgtaatct tgccaggagt gcggtcgctc
     6421 ccgtcattgg tctcaacgac gattgtagca aggtttgata atgcgccgcc ttgcgcgccc
     6481 ggagcgccgc cgggtcgagc tcgagtcctt ggtgcgccac ccagtcgtcg atccccttac
     6541 caagccgcac ccagtgatcg caatattccg cttcagcgag ctcgatgttg tcttcgagca
     6601 gcgccatccg ataggcccgg caatggagtt cttcagtgtc ggccagtagg ccgtcgaggt
     6661 caaaaataac cgtggtgatc atcgttatta tcggtgattt tactgaactt tcgccgacat
     6721 tgcatcagct tgatgttgcc tccgttgggc gaccaaaaaa aaacggccaa gctgcgcgag
     6781 cttggccgtt tgtctataaa ttattgcccg aggtctaaga ctgctgcctg ggcattttgc
     6841 ggaagttttc gtggcgcgcc gccaccaagg ttttgagttg gtcgcgctgc tggacggaca
     6901 gcaccgcctg gccatggtcc aaggcttcgt actgtaagta gcgcagttcc gaacggtatt
     6961 tttccgtttc ctggatcttc gacttgatct taaccagatc agccggcgat tgctccctca
     7021 atccggcgat ttctttttcg atggtgcgca tctgttggtg cagcggagtt acctgagtct
     7081 gaaaattgct cttgatctgc tccagtttag cgacctgatc gttactcagg cttagctcgc
     7141 tcttatgctt gagcgcgatg ctgattaacg gcgcaccgcc agcaaatccg ccggccatat
     7201 gggcgccacg ctcaacgcgc ctgccgccgc cctctggcgc ggccgccatc ggcgcagaga
     7261 cgagcgcgac tgtcgccaga gccgcgccac cgaagattaa cgaagaaacc ttgttcataa
     7321 accctccttc agggtgtaag taatttgatg gttagacggc gccaaagcaa aagggtttac
     7381 cttcggtcgc caattacttg ttgagctaca tgtccgaccg cgctgggggg gggaattgtc
     7441 aaggcgtttg cacactcagc gcacgatcca atagccggtc gaacatgacc tgaggcttga
     7501 agaccttagc tcctcgctta ccaacggcga tcgcgctggc gctattagaa tgcgacgggc
     7561 tcgattgtcg ctgcttggtc aagctgaacg gccttgttgg attaataatt atggtgtaat
     7621 agtccggcca gctatttttg cggagaaggt ttatggaaaa tcaggtcgtc ggtggaaacc
     7681 tctcgggacc gcggttggca tatctgcatc atgtgagcct gccttgccgc gatttggcag
     7741 agtcgaagcg tttttatatc gaagtcttgg gcggcgaact ttatcacgac acatctggtt
     7801 tcgccgaagt gatgcttgcc gacatgatcg ttgggatgtc ggaacaagcg gggggttgga
     7861 cggggtggga agccgaatat cc
//
LOCUS       DASQKS010000050         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2965974, whole genome shotgun sequence.
ACCESSION   DASQKS010000050 DASQKS010000000
VERSION     DASQKS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965974"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..468)
                     /locus_tag="VE170_01315"
     CDS             complement(115..468)
                     /locus_tag="VE170_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14097.1"
                     /translation="MSEPAISNIASAKIAVVEVLNVGGKKARFATELLAGLRRHNVAK
                     EEMDRALVELEAEGTVVVRENFCADPHLAEVDLRVIGLVDTGRSDNAHAAALQQIDLA
                     WNKWLGEYLANHRCG"
     gene            complement(565..1545)
                     /locus_tag="VE170_01320"
     CDS             complement(565..1545)
                     /locus_tag="VE170_01320"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14098.1"
                     /translation="MKTTRSWIEMALLLLVILASTAVMAQDKLTRVRIAYPSGGICCL
                     ALYGGIQWKIFEENGLHVEIVQIRSQIGNMALMANEIQYFSGVGPASVSATLRGMPSR
                     GVWFASDQLIYSLMARSEIQSLRDLRGKKIGITGLGGTSHVALQIALEAAGENPKNFA
                     YVVLGAPQQLPALETGTVDAAIFNPPFDFYAKRKKFREVLDVGAKVKMPLGGLTTMLS
                     TIRNRPDEVRRVLRSMQLAKQEVLRSKEKATAMILKYLQVDKEAADDTFEALKRTVSG
                     SGVPTRDGMDLIIKSLQAAGQFTDRKVAFEEIADDRIAKEVAKELGYKVQ"
     gene            complement(1624..2619)
                     /locus_tag="VE170_01325"
     CDS             complement(1624..2619)
                     /locus_tag="VE170_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14099.1"
                     /translation="MKPYLALPIGLLLTGGFVMPASAQPLLKIRVGYPSISSRQAQLW
                     IAKDEGLFKKYGLDVELIFLRGGQVAIQALTGGDPPLMTVGNVIVANLQGHDIVLVGA
                     VENSYDQSVMARPGTMTRMEQLKGKRFGISGFGSATHNAALILFKKFNLEPNKDVIFV
                     PTGTGPERLAAMSAGRIEATFFNPSEVPQSLKAGFVEIIQMADLDFEVQGSGLATSRS
                     YIKSHRDTVKSALKAYIEGIYFVFANKAAAVKSLAKYMRTNDAEVLDYSYQHYLKRTP
                     KKPYPTMKGIQNLVDMSVPQVPQAKTAKPEQFVDLSFLQELENEKFFDEMAKRYR"
BASE COUNT          602 a          808 c          705 g          625 t
ORIGIN      
        1 aatcgttcca ggcgctaaaa agttcaaatc gccggacaac aagtaaaacc ttagaaagcg
       61 gattaaacta cctaaatact ttgaactatt tgaacgcgcg gcgccgatgc cgcgctagcc
      121 gcaccggtga ttcgccagat attcgcccag ccacttgttc caggcgagat cgatttgttg
      181 caacgccgcg gcatgagcat tgtcgctgcg gccagtgtca accaaaccga tgacccgtag
      241 atcgacttcg gcgagatgcg ggtcggcgca aaagttttcc cgcaccacca ccgtaccttc
      301 agcttccaac tcgaccaaag cgcgatccat ttcctccttg gcaacgttgt ggcgccgcaa
      361 cccggctagc agctcggtcg caaagcgcgc cttcttacca ccgacattga gtacctcgac
      421 gactgcaatc ttcgcactgg cgatgttaga aatcgctggt tcactcatgg ttcaattcca
      481 actgccacac gtcatattcc aaattgccgt caagagcaga acttacgtgc agccaatttg
      541 gaattgtatt atggaatttg tgattcactg caccttgtac ccaagctctt tggccacttc
      601 cttagcgatg cgatcgtcgg caatctcttc gaaggcgacc ttgcggtcag tgaactggcc
      661 ggctgcttgc agcgatttaa taataagatc cataccgtcg cgggtcggca cgccgctgcc
      721 gctgacggtc cttttcagcg cctcgaaggt gtcatcggcg gcctccttgt cgacttgcaa
      781 gtacttcaaa atcatcgccg tggctttttc cttggagcgc aacacttctt gcttggccag
      841 ctgcatcgag cgcagcacgc gccgcacttc atcgggtcgg ttgcgaatcg tcgagagcat
      901 cgtcgtcaaa ccgcccagcg gcattttcac tttggcgccg acatcgagca cttcacggaa
      961 ttttttgcgc ttggcgtaga aatcgaacgg cgggttgaat atcgccgcgt cgaccgtgcc
     1021 ggtctccagc gctggcaact gctgcggcgc acccaaaacc acataggcaa agtttttggg
     1081 attctcaccc gccgcttcaa gggcaatttg cagggccacg tgagaagtgc cgcccagacc
     1141 ggtaatccca atcttcttgc cgcgcagatc acgcagcgat tgaatctccg accgcgccat
     1201 taaagaatag atcaactgat ccgacgcgaa ccagacgccg cgcgacggca tgccgcgcaa
     1261 agttgcgctc accgacgccg ggccgacgcc ggaaaagtat tgaatctcat tagccatcaa
     1321 cgccatgttg ccgatctgtg agcggatctg cacgatctcc acgtgcaggc cgttctcctc
     1381 gaagatcttc cattgaatgc cgccgtacaa cgccagacag caaatgccgc cgctcggata
     1441 ggcgatgcgc acgcgggtga gcttatcctg cgccatcacc gccgtactcg ccaagataac
     1501 gagcagcaac aaagccattt cgatccaact tctggtggtc ttcatagcgg tgtccaattt
     1561 cgcgttggca aaagccatcc gtttccgacg aaattctcca ttatccattt tccattccct
     1621 ccgtcaccga taccgtttgg ccatctcgtc gaaaaatttc tcgttctcca attcctgcaa
     1681 aaagctcaag tcgacgaact gttccggctt ggcggttttc gcctggggca cctgcggcac
     1741 ggacatgtcg acgagatttt gaatcccttt catggtcgga taaggtttct tcggcgtgcg
     1801 cttcaaataa tgctgatagg aatagtcgag cacctcggca tcgttggtgc gcatgtactt
     1861 ggccagactt ttcaccgccg ctgctttgtt ggcgaaaaca aaataaatcc cttcgatata
     1921 ggctttgagg gcggacttga cggtatcgcg atggcttttt atgtagctgc gcgaggtcgc
     1981 caagccgctg ccctgcactt cgaaatcgag gtcggccatt tgaatgattt caacaaagcc
     2041 cgcctttaga gattgcggga cctctgatgg gttgaagaag gtcgcttcga ttcttccggc
     2101 gctcatcgcg gcgagccgtt ccggcccggt gccggtcggg acgaagatta cgtccttgtt
     2161 gggttccaga ttgaatttct taaacagaat cagcgcggca ttatgcgtcg ccgagccgaa
     2221 gccgctgatg ccgaaacgtt tgcccttgag ctgctccatg cgtgtcatcg ttcccggacg
     2281 cgccatcacg ctctgatcgt aggagttttc caccgcgccg acgagcacga tgtcgtggcc
     2341 ctgaaggtta gcgacgatga cgttgccgac ggtcatgagc ggcggatcgc cgcccgtcaa
     2401 cgcctggatc gccacttgtc cgccgcgcag aaaaatcagc tcgacatcga ggccatattt
     2461 cttgaacaat ccctcgtcct tggcgatcca aagttgcgcc tggcgcgagc tgatggatgg
     2521 atagccgacg cggatcttta gcagcggctg cgccgaggca ggcatcacga acccgcccgt
     2581 aagcaacaat ccgatcggca gcgccaagta aggtttcata aggctatcct ctgatgtatg
     2641 tcatcgatgc cgaaccgtca aatgagcgca gcctggcatg gcacaacgtc aaccaaataa
     2701 taagggctca tcgcgtaatc gagtgggtcg attttaataa
//
LOCUS       DASQKS010000051         6441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_446664, whole genome shotgun sequence.
ACCESSION   DASQKS010000051 DASQKS010000000
VERSION     DASQKS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446664"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..238
                     /locus_tag="VE170_01330"
                     /pseudo
     CDS             18..238
                     /locus_tag="VE170_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540917.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
     gene            759..1154
                     /locus_tag="VE170_01335"
                     /pseudo
     CDS             759..1154
                     /locus_tag="VE170_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            1291..1755
                     /locus_tag="VE170_01340"
     CDS             1291..1755
                     /locus_tag="VE170_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14100.1"
                     /translation="MAGKVDPIPDDYRGATPYLCVNDGVGAIEFYKKAFGAREVMRMP
                     MPDGKLGHAELRIGAAPIMLADEHPEMNFRSPHSIGGTPVNIVVYVNDVDALVKQAEA
                     AGAKILRPPENQFYGDRMATLEDPFGHSWSFATHIEDVSPEEMQKRAAAPHS"
     gene            2202..3842
                     /locus_tag="VE170_01345"
     CDS             2202..3842
                     /locus_tag="VE170_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14101.1"
                     /translation="MHLMIVFFVLTLSLSMAAAQHQHGNQPAKVSELHPGLGNYHHPI
                     TTKSAEAQSYFDQGLTLLYGFNHDEAARYFQRAAELDPEAAMPYWGIALATGPNYNDT
                     AVDDNRAKSTHAAVRQALQRAPKASARERDYIRALQKRYPSANPKSDWKKLHRDYSDA
                     MRAVVKKYPDDLDAATLFAESLMMLRPWQLWTDGKPAPGTLELVAVLESVLKRNPHHP
                     GANHFYIHAVEASANLERAIPSAERLMTLVPGAGHLVHMPGHIYLQTGDYELAAQANV
                     KASDADKAFVERTGATGMYPLMYYTHNIHFISYARAQQGRYDEAKQAALQMVQNVGNA
                     DAQMQMLEGFHLYPLMIDLRFQRWDDILKAEDPGPERKLHRSFRHYARAMALAGQGKI
                     AEAAAERRLFETTRKGVPPESQFLINNKAADILALAAATLDARLAWADGQKDKSIEAW
                     RRAVKLESKVQYDEPPAWFYPVRQSLAAALLRSGRAKEAEAVFRAAMDKQPRDGRLLF
                     GLWQSLMAQKRDSEATLVEQQFVAAWKDATVKLNIEDL"
     gene            4050..4457
                     /locus_tag="VE170_01350"
                     /pseudo
     CDS             4050..4457
                     /locus_tag="VE170_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
     gene            4500..4928
                     /locus_tag="VE170_01355"
     CDS             4500..4928
                     /locus_tag="VE170_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HYJ14102.1"
                     /translation="MAVAGLKRSYRFEDRSKIPLQWESFAPSIGKVPGQVGKVAYGVC
                     LNYRAGHGFDYLSGVEVNDGAKLPPEFAAVRLPAQEYAVFTYPHHVSAIATTIDKIWN
                     SWAPQCGLRIKQSPWFERYTAEFDPQSGMGGMEICIPIEE"
     gene            5118..>6441
                     /locus_tag="VE170_01360"
     CDS             5118..>6441
                     /locus_tag="VE170_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYJ14103.1"
                     /translation="MDLLHSQYQALLEISEAIASHRELEPLFRDLAPRLHRVVQFDFA
                     NLLLYEPDRKALKSHVLETPDPAYTCPAGDCPMEAPGGWVWQTQKPWVVSRLADDSKF
                     PELSSWLGGRGVQSLCIVPATTALRNLGALAFGSTHEAAYAEADVIFLQQVARQVAVA
                     IDNAHNFAEAQSVHKELEAERDRLSLLLEVNNSVVSVLDLHDLLKAVSDSLRRLVPHE
                     YASLSLYDAESQSLQIHALDFPVSKGLLQEGLSIPLVGSPTGRALQTRQPVLITRSDI
                     EQFGSDIARRILAEGLKSAYCLPLISHDRPLGTLVVASLTDDVFPEKDARLLQHVANQ
                     IAIAVENSLAFRRIVERANKLSEEKLYLQDEIRTKYNFEEIIGESAALKRVLDQIQTV
                     APTDSTILIQGETGTGKELIARAIHHLSERRERTLVKVNCASIPTGLLE"
BASE COUNT         1465 a         1825 c         1831 g         1320 t
ORIGIN      
        1 ttccatcgga ggatgatatg gccgtggctt gtgaacatct gacgggattg acccccgcgg
       61 actttccgcc gcaaaagact gccaacgctt gtgcggaatg tctgagggag ggcacttttt
      121 gggtcgcgct gcgcgagtgc caaacctgcg gccacgttgg ctgttgcgat tcttcgacgg
      181 gcagcatgcc accagacact ttaatgagac ccagcatccg gtcatgcgcg ccgtcccgcc
      241 cgcagctaaa aagctcggca aggtcacttc ctatattttt tatctaagga tgattcaagc
      301 gcggcaagtc gatgtcttgc gtttcactct acagggagtc ggatcgatcg gtacggtgct
      361 gtcggaattt gccggattca tgttttttag ccttatctgt ttgtcactgg ccgtaaaagc
      421 gctcgatcgc gcccgctagt aaacagctgg cgggtgaaag cctgaaaaac aagattagga
      481 tcgaagcaga taacagaccg cagcgcgcga tcgcgtgtca cacaatcgca acacaaaatt
      541 taacttgctg tctctctttt gagagcaacg ttgatgtcaa ccttcgtcat tgggtttttg
      601 ggtttttcga tgagtatttc tctgtgtcga ggggacatta tttgcgtatt ttcttgatgt
      661 aaaatgattt cctgctaaac ggttatttgt gcccccagat cgtggggtgg gcctcgcttc
      721 caagtgacag atttatcaca agagaaagga gcatgatcat ggctattcag ctgaaccaca
      781 ccatcgttgc cgcgcgcgat aaggccgcct cggcaatgtt ctttacggag attttcgctc
      841 ttcccccggc cgttaatgtc gggccctttg cagtgatcca ggtgggcgat acctcgctgg
      901 attatatgaa tgtcgaggag taccggcccg gcgcccagga cgaaatcact tcgcagcact
      961 atgctttttt ggtgagcgaa gcggaattcg acgagatctt cgcgcggatt cgtgaccggc
     1021 gcctgacata ctgggcggac cccggacgca acaagcgtga ccagatcaac acctgggacg
     1081 gcgggcgcgg cgtctacttt aacgacccca acggccacct gttggaaatt atcacccggc
     1141 cctacggcag cggcggttcg acggcatcgc tagttcatcc tttgttcgcg gccgctgacg
     1201 atcacgaggg tcgcggcggc tcacggtgat aacggtctct atccgagcga taaccaaaac
     1261 tttaaaaacc aataagaggg agatattttt atggcaggaa aagtagaccc gatcccggac
     1321 gactaccgcg gtgccacgcc atacctatgt gtaaacgacg gcgtgggcgc tatcgagttc
     1381 tacaaaaagg cgttcggcgc gcgcgaggta atgcgcatgc cgatgccgga cggcaaactc
     1441 ggtcacgccg agcttcgaat cggcgcagct ccgattatgc tcgccgacga gcatccggag
     1501 atgaactttc gcagtccaca ttccataggc ggaacgccgg tgaatatcgt tgtttacgtc
     1561 aacgacgtgg atgcgctggt gaaacaggcc gaagccgcgg gcgcgaagat actaagaccg
     1621 cccgagaatc agttctacgg cgaccgcatg gcgacgttag aagacccgtt cggccattcg
     1681 tggtccttcg caacccacat tgaagacgtc tcgccagagg aaatgcagaa gcgcgccgcg
     1741 gccccgcata gttagtcgat aaagcgttac tgaataattg ccgcactgtc ttgaatcttt
     1801 gtggacaccg atctcgaccg acgaaagaac gggttcaagt cctggggtgg ggagcattca
     1861 attgaatgga tatcgacggc tcgctgattt gcacaggtaa gacgcgatca atcaccagat
     1921 cgccgacaaa cccgacgcag ggcatgaggc ataagccgga tattctgggc gatcctcgaa
     1981 tagcgcgacc cggatcaagt ctaatcaaag aatttgaatg ttgggcgccg gcattgcggc
     2041 tcaaaaatcg agagaaaccg tcattgtgca aaggcggagc ggttttcatt tcgcatgaag
     2101 tgagaaatcc ttctttgcgt ccgcgcattc gcccgtgatg agggcgctgt atccggatga
     2161 agtagaacgt cgacgctacc agaaaaataa ggagggaaga aatgcatttg atgatagtct
     2221 ttttcgttct gactctttcg ctttccatgg cggcagcgca gcaccagcat ggcaatcagc
     2281 cggcaaaagt ctctgagctg cacccgggat tgggcaacta ccatcatccg atcacgacca
     2341 aaagcgccga agcgcaaagt tacttcgacc agggactgac gcttctctac gggttcaatc
     2401 acgacgaagc cgcgcgctac ttccagcgcg ccgctgagct cgatcctgaa gcggcaatgc
     2461 cttactgggg tatcgccctc gccaccggac cgaactacaa cgatactgcg gtggacgata
     2521 accgcgccaa atcgacccac gccgcggtac ggcaagcgct ccaacgcgcg cccaaggcga
     2581 gcgcccgcga gcgcgactac attcgcgccc tgcaaaagcg ctacccttcg gcaaatccga
     2641 agtcggactg gaagaaacta caccgcgact acagtgacgc catgcgcgcc gtggtcaaaa
     2701 agtatccgga cgatctcgat gcggccaccc tgtttgccga gagcctgatg atgctcaggc
     2761 cctggcagct ctggaccgac ggcaagccgg cgcccggcac tctcgagctc gtcgcggtgc
     2821 tcgagtccgt gctgaaacgc aatccgcacc acccgggcgc caatcacttc tacattcatg
     2881 cggtggaagc ttcggcaaat ctcgaacgag cgattcccag cgcggagcgg ttgatgacct
     2941 tggttcccgg cgccggccat ctggttcata tgcccggcca tatctatctc cagactggcg
     3001 attatgaact ggcggcacag gccaacgtca aggcgtcgga tgcggacaag gcgtttgtcg
     3061 agcgcaccgg cgccaccggc atgtatccgt tgatgtacta tacccacaac attcacttca
     3121 tctcgtacgc gcgcgcgcaa cagggacgct acgacgaagc caagcaggcg gcgctgcaaa
     3181 tggtccaaaa tgtcggcaat gccgatgccc agatgcagat gctcgagggt tttcacctgt
     3241 acccattgat gatcgacctg cgcttccagc gctgggacga tattttaaaa gctgaggatc
     3301 cgggtcccga gcgcaagctc catcgctctt tccgtcatta tgcgcgtgcg atggcgctgg
     3361 ctggacaggg caagatcgcc gaagcggcgg cggagcgcag gctgtttgaa accacgcgca
     3421 aaggggtgcc gccggaatcg caatttctca tcaataataa agcggcggat attttagccc
     3481 tggccgcggc caccctcgac gcccggcttg cttgggccga cggccaaaaa gacaagtcca
     3541 ttgaagcatg gcgccgggcg gtcaaacttg aatccaaagt ccaatacgac gagccgccgg
     3601 catggttcta ccccgtgcgc caatccttag ccgccgcgtt actgcgcagc ggtcgcgcta
     3661 aagaagcgga agcggttttt cgggcagcca tggataagca accgcgtgac ggccggctgc
     3721 tgtttggcct gtggcaaagt ttgatggcgc aaaaacgcga cagcgaggcc accttggtcg
     3781 agcagcagtt tgtcgctgcc tggaaggacg ccaccgtgaa actgaacatc gaagatctgt
     3841 aaaaataact cgcgaaaacg ctggtaatct ccgacttaaa cggaacgctg gacggcgcgc
     3901 tggaaagtta atggccggct aaattgcagt gattgcagtt aaattacctt tgtcgatttg
     3961 gcaactcggt aaacgactac tcgatgtaac caccggtagt ctggctgaat cctggcgaac
     4021 cggaaactta accaataggg agaacgtcaa tgcgctacat gatcttgatt aaagccagca
     4081 aagattccga agcgggtgtg atgcccagcg agccactgct gactgcaatg ggcaacttca
     4141 acgaagaact ggtgaaagcc ggaattatgc aggccggcga gggccttcac cccagctcga
     4201 acggcgcgcg cgtgcgcttt tccggaaaca ccaggacagt gatcgatgga ccctttgccg
     4261 agactaaaga actgatcgcc ggattctgga tctggcaagt aaagtccaag gaagaggcga
     4321 tcgaatgggt caagcgctgt cccaaccccc atgaggaaga ttacgaagtc gagattcgcc
     4381 gggtgttcga ggccgaggac tttggcgcgg agtttactcc cgaactcaga aggcaggaag
     4441 ccgggattcg cgcccaagcg ctgggcctgg gcgtgcttcg cttcgagccg gggcgggaaa
     4501 tggcggtggc gggattgaag cgcagttata ggttcgaaga ccgcagcaag attcccctgc
     4561 aatgggaaag tttcgccccg tccatcggca aagttcccgg acaggtgggc aaggtcgctt
     4621 acggcgtctg tttgaactac cgagccggcc atggattcga ctatctttcc ggcgttgaag
     4681 tgaacgacgg cgccaagctg ccgccggagt tcgccgccgt acgcctcccg gcccaggaat
     4741 atgcggtctt cacctacccg catcatgtat cggcgattgc cacgaccatc gacaaaatat
     4801 ggaatagctg ggcgccccaa tgcgggctaa ggatcaaaca atcgccgtgg ttcgaacgct
     4861 acaccgctga gtttgatccc caaagcggta tgggcggaat ggagatctgc attcctatcg
     4921 aggagtgaac cgttcgtcgg gcgtgaaatc aacccgttaa agtgggcaag ttccatgaat
     4981 ttgccgatgg cggtgagacc cacatggcgc caaccaggcc cttctaaccg taactgtttt
     5041 ctgtttggcg tcttgatagg caacccatcg gcatataatc ttactgcctt gagaaacgat
     5101 ttatcgagcg cccagcgatg gatcttctgc acagccagta tcaagcgctt ctagagattt
     5161 ccgaagcgat cgcatcgcac cgggaactgg agccgctgtt tcgcgacctc gcgccgcgtc
     5221 tgcatcgcgt cgtgcagttc gattttgcca atttgcttct ctacgagccc gatcgcaagg
     5281 cgttgaagtc ccatgtgctc gagacccccg atccggctta cacatgccct gccggagact
     5341 gtccgatgga ggcaccaggg ggttgggttt ggcagacaca aaagccgtgg gttgtttccc
     5401 gtttagcaga cgacagcaaa ttccccgagc tatcaagctg gctaggcggc cggggcgtgc
     5461 aatcgctttg catcgttccg gcgaccacgg cgctgagaaa tttaggggcg ctcgcctttg
     5521 gcagcacgca tgaggccgcc tacgccgaag ccgacgtcat ctttctgcag caggtggccc
     5581 ggcaagtcgc cgtggcgata gacaatgcgc ataattttgc cgaagcccaa tcggttcaca
     5641 aggagctcga agcggagcgc gatcgcttga gcctgctgct cgaggtcaac aacagcgtcg
     5701 tttcggtgct cgatttacac gacctgctca aggcggtttc cgattcctta cggcgcctgg
     5761 tgccgcacga atatgccagc ctctccctct acgacgcgga atcccagtct ttgcaaattc
     5821 atgcgttgga ttttccagtt agcaagggat tactccagga aggtctctcc attcccctcg
     5881 tcgggtcgcc cacgggacgc gcgctacaaa cccgccagcc ggtcttgatc acgcgcagcg
     5941 acatcgagca gttcggttcg gatatcgctc gacgcatact cgccgaggga ctcaagtcgg
     6001 cctactgcct gccgctgatt tcccatgatc ggcccttggg aaccctggtg gtggcgagcc
     6061 tgacggatga cgtctttccg gaaaaggacg ctcggctgct gcaacatgtc gccaaccaga
     6121 tcgccatcgc cgtggaaaac tccctggcgt ttcgccgcat cgtcgagcgc gccaacaaat
     6181 tgagcgaaga gaagctctat ttgcaagacg agatccgcac caaatacaac ttcgaagaga
     6241 tcatcggcga gagtgccgcg ttgaaaagag ttctggatca aattcagacc gtcgccccga
     6301 ccgattcgac gatcctgatt caaggcgaaa ccggcacggg aaaagaattg atcgcgcgcg
     6361 cgattcatca cctcagcgaa cgccgcgagc gcaccctggt caaggtgaac tgcgcctcga
     6421 tcccgaccgg tcttttggag a
//
LOCUS       DASQKS010000052         5457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_866804, whole genome shotgun sequence.
ACCESSION   DASQKS010000052 DASQKS010000000
VERSION     DASQKS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866804"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VE170_01365"
     CDS             <1..219
                     /locus_tag="VE170_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HYJ14104.1"
                     /translation="LIDELKAHVSKKIGPIARPDEILFTAELPKTRSGKIMRRLLRDI
                     ADGRAMGDTTTLADPTVVAKLKEQYEED"
     gene            267..1259
                     /locus_tag="VE170_01370"
     CDS             267..1259
                     /locus_tag="VE170_01370"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14105.1"
                     /translation="MGRNRILLMAFLLFLPQLVTAQTREKVRVALGSISVNTSVIPVG
                     QQYGLYAKYGVDLEPIYMGGGMNSLAAVTSNSVQFLSAGSTATISARLGGMDITMLTV
                     QSSKLEYSIFAAPDIKTPQDLKGKIVTGTRPGASADSALRLILRKWGLEPGKDVIFIS
                     VGESQQGRMNALQRGSVSATALTPPFSGMARQLGFRELIDMRKTDIEYAGSAIAGMVG
                     YIKSHPQLVENFLKGYIESLHFFRTQKEKTVAGIMKFMKMNDRSRAEEGYEYYLDLMP
                     VMPYASAAGVKAVLQFLAAGQPKAATANPEDFYDHTILKKIEDSGFAKPFAIRR"
     gene            1294..2292
                     /locus_tag="VE170_01375"
     CDS             1294..2292
                     /locus_tag="VE170_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14106.1"
                     /translation="MQHKTILRVLLAGFCILTAARELPAQGLKKIRWGQTSIGASQWI
                     PWIAKDMKFYEKNGLDMEIILLRGSGQTSQAMIGGSIFASPVTTATLMTANLSGADLT
                     TVAHTVSVVQGRMLVKPEIRKAEDLKGKKVASSGLGSLGDFMYRVIMRKYGLNPDKDI
                     NWITVGTPPERLQALAAGSVDAADLSYPSDVRGEKMGFRVLWDARAEVPYPSMSVVTR
                     KKTIQEDRDSVMRMMRAHVEGIHFLKTQKEASLKILAKYLRTDDRELLDGSYDIYSKD
                     FIPTPYPIVTGLKLAYEQVAQRRPEIYNHKPEEFVDASFISELDKSGFIKKLYAQR"
     gene            2337..3344
                     /locus_tag="VE170_01380"
     CDS             2337..3344
                     /locus_tag="VE170_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14107.1"
                     /translation="MKWIAQSAFTSLLICLAAFASPGLGQDKPLKKIAWGQTSISSSQ
                     WIPWIAKDAKLYEKNGLDVDIVYLRGGSGDTSKAIIAGSIFAAPVTTATLMTANLGGA
                     DLTTIAHTVKAVQTKLIARPELKRPEDIKGKRLAMTGLGSLGDFLHRVVMKRHGINPE
                     RDVTWLTIGAPPDRIQALEAKLVEAADLSYPFDVQAEKKGYRVLWDARTEVPYPSMSV
                     VTRKRTVQEDRDTVARMMRAHVEGIHFLKTQKESSLKILAKYLRTTDKELLEGSYEIY
                     KEDFLATPYPITQGLATTYEYVALRRPEIYNHKPEEFTDPSFIAELDKSGFIKKLYAS
                     K"
     gene            3382..4386
                     /locus_tag="VE170_01385"
     CDS             3382..4386
                     /locus_tag="VE170_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14108.1"
                     /translation="MKVRAKNTLLALLLPALVFQSIAQAQDRPLKKINWGVTSLSAGN
                     WIPWLAKEAKIYEKHGLDVQLVLLRGSGQTSAALLGGSIFAAPVVLPTLMLADLSGAD
                     LINVAHTVSAVNSKLVVKPEIKRVEDMRGKRIANSSLGSLGDFLFKHILRKYGLDPIR
                     EVTWLAIGTPPERLQALFSGVVDAIEASYPIDVQAERKGFRVLLDARKEVVYPSMSIV
                     TRRKSIVEDRDTVMRMVRAHVEGIAYFKQNKEFSLRVLSKQLKIADRELLESSYDTYT
                     QDFISVPYPITKGLEATYDFVAQSRPEIRNRKPDEFVDTSFIAELEKSGFIKKLYEQK
                     "
     gene            4447..>5457
                     /locus_tag="VE170_01390"
     CDS             4447..>5457
                     /locus_tag="VE170_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14109.1"
                     /translation="MRKRRIQKVFSGNLKSKSGPADKNLKWLGSVTLFIALVIFGGAL
                     EAQQPTKIPRIIYLSGSPLSANAARYEAFRQGLRELGYMEGKNIVIEYRYAEGKPDSL
                     SDLAAELARLKVDIIVSAGAQATGAAKEATNTIPIVMTNEGDPVGTGFVASLAQPGGN
                     ITGLSTLAPEISGKRLELLKEIVANLSRVAVFGTSIHPGNAQSLKETELAGAALNVQL
                     QFLDVREPHDIETAFRAASKGRAGAVLVLAGAVLNSQRKQMAELAVKHRLPAIYPWPE
                     SVQDGGLISYGVNRNDLDRRAATYVDKILKGRKPADLPVEQPIKFEFITNLKAAKQIG
                     LTI"
BASE COUNT         1289 a         1472 c         1581 g         1115 t
ORIGIN      
        1 ttgatcgatg agctgaaagc tcatgtgtcg aagaagattg gaccgattgc ccggcccgac
       61 gaaattcttt tcacggcaga gttgccgaag actcgcagcg gcaagatcat gcgccgcttg
      121 ctgcgcgata tcgccgatgg acgggcgatg ggcgacacga cgaccttggc cgacccgacg
      181 gtggtggcca agttgaagga gcagtacgaa gaggattagg cgaggcagcg ggcattagac
      241 aataatatta gatcagaagg atacccatgg gtcgcaatag aatactgcta atggctttct
      301 tgctattttt gccgcagctg gttacggcgc agacgcggga gaaggttcgc gtcgcgctcg
      361 gctcgatttc cgtcaatacc agcgtcattc ccgtgggtca acaatacggg ctctacgcca
      421 agtacggcgt cgatctcgag ccgatctaca tgggcggcgg gatgaactcg ctggcggcgg
      481 ttacttcgaa ttccgtgcag tttctttccg ctggctcgac ggccacgatc agcgcgcggc
      541 tgggcggcat ggatatcacc atgctgaccg tgcagtcgag caaactcgag tacagcatct
      601 tcgccgcgcc ggatattaaa accccccagg atttgaaggg aaagatcgtc accggcacgc
      661 ggccgggagc gtcggccgat agcgccttgc gtttgatttt gcgcaagtgg ggtttggagc
      721 cgggcaagga tgttatcttt atttccgtag gagaaagcca gcaggggcgc atgaatgcgc
      781 tgcagcgcgg ctcggtttcg gccaccgctc tgacgccgcc attcagcggc atggcaaggc
      841 agctcggttt ccgcgagctt atcgacatgc gcaagaccga catcgagtat gccggttcag
      901 ccatcgccgg gatggtgggt tacatcaaaa gccacccgca gctggtcgaa aattttctta
      961 aaggttacat cgagtcgctg catttctttc gcacccaaaa agaaaaaacc gtcgcgggca
     1021 tcatgaagtt tatgaaaatg aacgaccggt cacgggccga agagggctat gagtattacc
     1081 tcgatctcat gccagtcatg ccctatgcca gcgccgccgg tgtcaaagcg gtcttgcagt
     1141 ttttggccgc cggccagccc aaagcggcca cggcaaatcc ggaagacttt tacgatcata
     1201 ctattctcaa gaagatcgaa gatagcggct ttgccaaacc gttcgcgatt cggcggtgag
     1261 gaaaattacg agaaaatttg gagtcataga ttgatgcagc acaaaacgat actgcgagtg
     1321 ctcttggcag gattttgcat cctcacggcg gcgcgggaat tacctgccca gggactgaaa
     1381 aagattcgct gggggcagac ttccatcggc gcatcgcaat ggattccctg gatcgccaag
     1441 gacatgaagt tttatgagaa gaacggcttg gatatggaga tcatcttgct ccggggttcg
     1501 ggacaaacct cccaggcgat gatcggcggc agcattttcg cttcgccggt gaccacggcg
     1561 acgttgatga cggcgaatct cagcggcgcc gatctgacga cggtggcgca tacggtctcg
     1621 gttgtccagg gtcgcatgtt ggtgaagccg gagattcgca aggccgagga tctcaagggt
     1681 aagaaggtcg ccagctccgg tttaggctcc ctgggtgatt ttatgtatcg ggtgatcatg
     1741 cgcaaatacg gtctcaatcc ggataaagac ataaactgga ttaccgtcgg cacgccgccg
     1801 gaacggctcc aggcattggc ggccggctcc gtggacgccg ccgatctgtc ctatccatcg
     1861 gatgtgcggg gcgaaaagat gggctttcgc gtgctctggg acgcgcgcgc cgaagtgccg
     1921 tatccgtcga tgtcggtggt gacacgcaaa aaaaccattc aagaagaccg cgacagcgtg
     1981 atgcggatga tgcgcgccca cgtcgaaggc attcactttt taaaaaccca aaaggaagcg
     2041 agcctgaaaa tcctggcgaa atacctgcgc accgacgacc gggaattact cgacggttcc
     2101 tacgatattt acagcaagga ttttattccg acaccttacc cgatcgttac cggtctcaag
     2161 ttggcgtacg agcaagtcgc ccagcgccgg ccggaaatct acaatcataa gccggaagag
     2221 tttgtcgatg cgagctttat ttccgaactc gacaagagcg gttttatcaa gaagctttac
     2281 gctcaaaggt gagatagccg cgcggcatga gcgcgaggcg cgaggagaat ccgatcatga
     2341 aatggatagc gcaatcggcc ttcacgagct tgctcatttg cctggcggct ttcgcttcac
     2401 ccggcttggg ccaggataaa ccactcaaaa agatcgcttg gggccagact tcgatttctt
     2461 catcccagtg gattccttgg atcgccaagg acgccaagct ctacgaaaag aacggcctcg
     2521 acgtcgacat cgtttatctg cgcggcggct cgggcgatac ctcgaaggcg attattgccg
     2581 ggagtatttt tgccgctccc gtcaccacgg cgactttgat gaccgccaat ctcggcggcg
     2641 ccgatttgac caccatcgct catacggtga aggcggtgca gaccaaactg atcgcgcggc
     2701 ccgagctcaa gcgacccgaa gacatcaaag gcaaacggct ggcaatgacg ggtttgggct
     2761 ccctcggcga ctttctccac agggtcgtga tgaaaaggca tggaatcaat cccgagcgcg
     2821 acgtgacctg gctcaccatc ggcgcgccgc cggaccggat tcaagcactc gaagcgaaac
     2881 tggtcgaagc ggcggatctc tcctatccgt tcgatgtgca ggccgagaaa aaaggttacc
     2941 gtgtattgtg ggacgcgcgc accgaagtgc cgtatccgtc gatgtcggtg gtgacgcgca
     3001 agaggacggt gcaggaagac cgcgacaccg tggcgcggat gatgcgcgcc catgtcgaag
     3061 gcattcactt tctcaagacc cagaaggaat cgagcctaaa gattctcgcc aaatatctcc
     3121 gcaccaccga caaggaactg ctcgagggtt cctacgaaat ctacaaagaa gattttcttg
     3181 cgacgcctta tccgattacc cagggcctgg cgacgactta tgaatacgtc gcgctgcgcc
     3241 gcccggagat ttacaaccac aagcctgaag agttcaccga cccgagtttc atcgccgagc
     3301 tcgacaagag cggtttcatc aagaagctgt acgcatcgaa gtagcaggcg aaaggcgcgg
     3361 atcgcgggga ggaaaccgat catgaaagtt cgcgcaaaaa atacactgtt ggcgctcctg
     3421 ttgcccgctc tggtgtttca atccatcgcc caggcacaag accggccact taagaaaatt
     3481 aattggggcg tcacctcgct gtcggcgggc aattggattc cctggctcgc caaggaggca
     3541 aagatttacg agaagcatgg cctcgacgtt cagctggtct tgctgcgcgg ctcggggcag
     3601 acttccgcgg cgcttctcgg cggcagcatc ttcgccgcgc cggtggtgct gccgacgctc
     3661 atgctcgccg acttgagcgg cgccgatctg atcaacgtcg ctcatacggt ctccgccgtg
     3721 aacagcaagc tggtggtcaa gccggaaatc aaacgagtcg aggacatgcg cggcaagcgc
     3781 atcgccaact cgagcttggg atctttgggc gattttctct tcaaacatat cctgcgcaaa
     3841 tacggcctcg atccgattcg cgaagtgacc tggcttgcca tcggcacgcc gccggaacgt
     3901 ttgcaggcgt tgttctccgg cgtggtcgat gccatcgaag catcctatcc catcgacgtg
     3961 caggccgaac ggaaagggtt tcgcgtattg ttggacgcgc gcaaggaagt cgtctatccg
     4021 tccatgtcga tagtgacgcg gcgcaaaagc attgtcgagg accgcgacac cgtgatgcgc
     4081 atggtcagag cgcatgtcga gggcatcgct tatttcaagc agaacaaaga gttcagcctg
     4141 cgcgtcttga gcaagcagtt gaaaatcgcc gaccgggaat tgttggagag ttcctacgac
     4201 acctacacgc aggattttat ctccgtgccg tacccgatta ccaaagggct ggaagccacc
     4261 tacgattttg tcgcccagtc gcgtccggaa atccgcaacc gcaagccgga cgagttcgtc
     4321 gacacgagct tcatcgccga gttggagaag agcgggttta tcaagaagct ctatgaacag
     4381 aaatgaagaa cgtaacaggc aatagtaata attttggatt ttcgattttg attgaggaca
     4441 caaaggatgc gcaaaaggag aattcagaag gtgttttccg gcaatctaaa atcgaaaagc
     4501 ggacccgcag acaaaaatct aaaatggttg gggtctgtca ctctctttat cgcattagtg
     4561 atctttgggg gtgcgctaga ggcgcagcag ccaacgaaga tcccgcggat tatttacctt
     4621 tcgggttctc ccctttctgc taatgcggcc cgctacgagg cattccggca aggtctgcgc
     4681 gagcttggtt acatggaagg gaaaaacatt gtcattgagt atcgatatgc agagggaaaa
     4741 ccagatagcc tgagtgacct tgcggccgaa ctagctcgtc tcaaggtcga catcatcgtc
     4801 tcggctggtg cgcaagcaac cggtgccgct aaggaagcga caaatacgat tccaattgtc
     4861 atgaccaatg aaggcgatcc agttggcacc gggttcgtcg ccagcctcgc gcaacccggt
     4921 ggaaacatca ctggactatc aacgcttgcc ccggaaataa gcggaaaacg actggagctt
     4981 ctgaaggaga ttgttgccaa tctctcccgc gtcgccgtct tcgggacttc gatccacccg
     5041 ggcaacgcac aatcgttaaa agagacggaa cttgccgggg cggcgctcaa cgtgcaactt
     5101 caattcttag acgtacgaga gcctcatgat attgagactg cattccgagc cgcaagcaag
     5161 gggcgtgctg gcgcagtcct tgtgctggcg ggcgctgtac tcaattctca gcgcaaacag
     5221 atggcggaac ttgcggtaaa gcaccggttg ccggcgatat acccctggcc ggaatctgtg
     5281 caagacgggg ggcttattag ctacggcgtg aacagaaacg acttggaccg gcgcgccgcc
     5341 acctatgtgg acaagattct gaaaggcaga aaacccgcag acctgcctgt agagcagccg
     5401 atcaagttcg agttcatcac caatctgaaa gcggccaagc agatcggcct gacgatt
//
LOCUS       DASQKS010000053         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1707359, whole genome shotgun sequence.
ACCESSION   DASQKS010000053 DASQKS010000000
VERSION     DASQKS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707359"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..731
                     /locus_tag="VE170_01395"
     CDS             24..731
                     /locus_tag="VE170_01395"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14110.1"
                     /translation="MKLIGLISPNSSEDTLADVYKIFPKDIRIEARTLHVGKYTDDEF
                     HRAELSFQGLVRDLVKQPLDFLMVTGELFLSYKGPGSDREILELVKQITPTPASTVLT
                     AVTRGCRALGLKCVVMASPFPEDQDERLVKFLAHDGIEVVAFRGLGCPNAEVIWQLAP
                     ETGYDLSMALLREHPEADGVYMPCNKWRTVSVIERIEKDSGKAVVTNTQAWTWEALHG
                     MGMGEPITGFGRLLRHQ"
     gene            complement(762..1628)
                     /locus_tag="VE170_01400"
     CDS             complement(762..1628)
                     /locus_tag="VE170_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14111.1"
                     /translation="MPKNGSGLASPTHATSATGRLPCCSKSLRRKFTSGASASHSPLL
                     DRAALSRVTLARSCLGVGHRRVVAIIFDPFNHSLAPWREIIRFSFASSAPSRSSRGGA
                     VDILTQTILAQHGVTANKDATLIVIGSPEELVTALRTGRIAACLLTQPRQLILYRDGF
                     TKLAYSGDYLSSYPTGGVGTTEEKIKNHPAEVLGFVRASLKGQQYFAQHRSESIEIIS
                     KYLAIKDRSLATEVYDLQLSRLGGLSYLDDAWMRGAIEFTKKSMNLTKEVAPSQVFDF
                     SFAAKALGRSKN"
     gene            complement(1543..1740)
                     /locus_tag="VE170_01405"
     CDS             complement(1543..1740)
                     /locus_tag="VE170_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14112.1"
                     /translation="MDDKAHVMEAYGTTIQLMFRVFFESFTAAKGNTEDERNAEERFR
                     AGVAHARHVRDRAVALLQQEP"
     gene            2044..3042
                     /locus_tag="VE170_01410"
     CDS             2044..3042
                     /locus_tag="VE170_01410"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14113.1"
                     /translation="MTKRALIVLLLLSLRADFSAAYGQATKLIVAYASPAATFCPGWI
                     ARREGIFAKYNLDVEMVLMQGASAYMPAMANGNIQILYGGGTAVSRAIATRDFDVMVI
                     ATETRYVPLRFMVNASIRSPADLKGKRIGAGLAGLDEYATLLYLEKLGLVVGKDVQMI
                     YLAGGIPSRVAAMKQGLIDGVAVNPPNEYELEKAGYRELVNFLDLKMPYAGVPHTVTK
                     TFRDRNRRVLEDYMTAHVAGMQLFRTNKQLAFKAIVELTRQKDPVILERTYDTYSKQY
                     EAIDGLPLPWQAGIESMITGFHERFNPQGIKNRDASAALDASFVQKAAERLMLGKK"
     gene            3159..>4203
                     /locus_tag="VE170_01415"
     CDS             3159..>4203
                     /locus_tag="VE170_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14114.1"
                     /translation="MQQVIKAVFFPAALWLCGATAAAVVVILCAIVPVDAASPLTRAV
                     ISYPNANPRVAPLWIAQDLDFFSKYGIKGEVVFVRNNQSLVAGIAAGDIDVGYTGGAT
                     VIGAAAGGLDLKMVAGFVSRGRGYLVVRPEISTPADLRGKRFGVISIGGAQWMYAMLA
                     LEQFGLNAVKDRVQFLIIGDPTALARALEANLIDATVFTTPSYNAKLKQQGLTMLAEL
                     TPPLATTGIVASRSFTLKNPETLENIMKALIEGLVFVLAPGNKSQVIKTLMNRFKISD
                     ASLVEPEYADALKDLEPKPYPTAEGVRNMQRLMQLQNPRLANINPASLIDSTIVRKLD
                     ESGFIRQLQARYGE"
BASE COUNT          905 a         1164 c         1220 g          914 t
ORIGIN      
        1 catgaacata tgaggagcaa accatgaaac tgatcggact gatttcgcct aactcgagcg
       61 aagatacgtt ggcggacgtt tacaaaatat tccccaagga cattcgcatc gaagcgcgga
      121 cgttgcatgt cggcaagtac accgacgatg aatttcaccg cgccgagttg agcttccaag
      181 gactggtccg cgatttggtc aagcagccgt tggattttct catggtgacc ggcgaactgt
      241 ttctgtctta caaagggccg ggctcggatc gggaaatcct cgagctcgtc aaacagatca
      301 caccgacgcc ggcttcgacg gtattgaccg cggtgacgcg cggttgccgc gcgctgggac
      361 tcaagtgcgt ggtgatggct tcgccattcc ccgaggacca agacgagcgg ctggtgaaat
      421 ttctcgccca cgacggcatc gaggtcgtgg cctttcgcgg cttgggctgc cccaacgccg
      481 aagtgatttg gcaactggcg ccggaaaccg gttacgattt atcgatggcg ctgttgcgcg
      541 aacatcctga ggccgacggt gtctacatgc cgtgcaacaa atggcgcacg gtttcggtca
      601 tcgaacggat cgaaaaagac agcggcaaag cggtggtgac caatactcag gcgtggacct
      661 gggaggcgct gcacgggatg ggcatgggcg agccgattac aggcttcggc cggctgttgc
      721 ggcatcagta agatggtggc tcgcgcgatg aacctagaag ctcaattctt gctgcgcccc
      781 aaggctttcg ccgcgaaact aaaatcgaac acttggctcg gcgcgacttc tttggtcagg
      841 ttcatgcttt tcttggtgaa ctcgatggcg ccgcgcatcc acgcgtcgtc gagatagctc
      901 aagccgccga gacggctgag ttggagatcg taaacttccg ttgccaagga acggtccttg
      961 atggcgagat acttggaaat gatttcgatg gattcgctgc ggtgctgcgc gaagtactgt
     1021 tgccctttca agctggcgcg gacaaagccc aagacttcag cgggatggtt tttgattttt
     1081 tcttccgtcg tgccgacgcc gccggtgggg tagctgctca aataatcgcc cgagtacgcc
     1141 agcttggtga aaccgtcgcg gtacaggatc aattgtcgcg gttgggtgag cagacaggcg
     1201 gcgatgcggc cggtgcgcag cgcggtcacc aactcctcgg ggctgccgat gacgatcaat
     1261 gtcgcatcct tgttcgcggt cacgccatgc tgggcaagaa tcgtctgtgt caggatgtcc
     1321 accgcgccgc cgcgtgatga gcgcgaaggc gcagaggacg caaaagaaaa tcggattatc
     1381 tcccgccagg gcgccaagga atgattaaac ggatcgaaaa tgatagccac aacccgacgg
     1441 tgacccactc ccaggcatga cctggcaagc gttacacggg atagggctgc gcgatctagt
     1501 agcgggctgt ggctggcgga tgcgccagaa gtaaacttgc gtctaaggct cttgctgcag
     1561 cagggcaacc gcccggtcgc ggacgtggcg tgcgtgggcg acgccagccc tgaaccgttc
     1621 ttcggcattg cgctcgtctt ctgtgttgcc cttagccgcg gtgaatgatt cgaagaacac
     1681 cctgaacatg agttgtatgg ttgtcccgta ggcttccata acatgtgctt tgtcatccat
     1741 ttttttgacc tccttctccg gaaattgatt tgccagcttt gagacggccg atttaatagt
     1801 taggacgcct gttattctga ctcgcgcttc tctaacgcca acaggtgatt gtaaagccgc
     1861 agaccgttcc gcattttatt taaaacgcga ggcgcgaact tgtctacagt cgaacgatcc
     1921 catcccgtgt ccgcgaatga atactggaca tattcattgt gcttggacta agttgtttcc
     1981 ggtgctgcag tgcgtgaacg cttgtcgatc aattaatcga atcgcaattt gaggccatag
     2041 tctatgacca aaagagcgct catcgttttg cttttacttt cgctccgggc cgatttcagt
     2101 gccgcctacg gccaggcgac caagctcatc gttgcttacg cttccccggc ggcgactttc
     2161 tgtcccggtt ggatcgccag gcgcgagggc atctttgcca agtacaatct cgatgtcgag
     2221 atggtcttga tgcagggcgc gtcggcctat atgccggcga tggccaacgg caacattcag
     2281 attctttatg gcggcgggac ggccgtgtca cgggcgattg cgaccaggga tttcgatgtc
     2341 atggtgatcg ccaccgagac gcgctacgta ccgctgcgct ttatggtcaa tgcctcgatc
     2401 cgcagcccgg ccgatttgaa aggcaagcga atcggcgccg gcctcgccgg tctcgacgag
     2461 tacgcgaccc tgctctattt ggaaaaactc ggcctcgtcg tcgggaaaga tgtccagatg
     2521 atctatctcg ccggtgggat tcccagccgg gtggcggcaa tgaagcaggg gctgatcgat
     2581 ggcgtcgcgg tgaatccgcc caacgaatac gagctcgaaa aggctggtta tcgcgagctg
     2641 gtaaactttc tcgacttgaa gatgccctac gccggcgtgc cgcacacggt cactaagact
     2701 ttccgcgacc gcaatcgccg cgtgctggaa gactacatga cagcccatgt cgccggcatg
     2761 cagttgtttc gcactaataa acaattagct ttcaaagcga tcgtcgagtt aacccggcaa
     2821 aaagatccag tgattctcga gcggacctat gacacctatt ctaaacagta tgaagccatc
     2881 gacggcctgc cgctgccttg gcaagccggt atcgagagca tgattaccgg cttccacgaa
     2941 cgattcaatc cgcagggcat caaaaatcgc gatgcttccg ctgctctcga tgcctccttt
     3001 gtgcaaaagg ccgccgagcg gctaatgctc ggcaaaaaat aagcgatagt gggtaagagt
     3061 cgtgggcaag acgggcaaac attgcacgtt agaagggcaa agcgcgtaaa aaaatccaga
     3121 attgttggcg acggctacgg tatttgcagg agtggtgcat gcagcaggtt atcaaggcgg
     3181 tgttttttcc cgccgcgctt tggctttgtg gcgcaaccgc cgccgcggtg gtcgtgattc
     3241 tctgcgctat cgttcccgtt gatgcggcct cgccgctcac gagagctgtc ataagttatc
     3301 ccaacgccaa ccctcgggtc gcgccgcttt ggatcgccca ggatctggat ttcttcagca
     3361 agtacgggat caagggcgag gtggtatttg tccgcaacaa ccagtccctc gtcgccggga
     3421 tagccgctgg cgatatcgat gtcggataca cgggaggcgc cactgtcatt ggtgcggcgg
     3481 caggcggtct cgatctgaaa atggttgcgg gtttcgtcag ccgcggccgg ggctacttgg
     3541 tcgtgcggcc ggagatttcc accccggccg atttgcgcgg taaacggttc ggcgtgatca
     3601 gcatcggcgg cgctcagtgg atgtacgcca tgctcgccct cgagcagttt ggtttaaatg
     3661 ccgtcaagga ccgagttcaa ttcctcatca tcggcgatcc gacggcgttg gctcgcgctt
     3721 tggaagcaaa cctgatcgat gcaacggttt tcacgacgcc gagctacaat gcaaaactca
     3781 aacaacaagg cttgactatg ctcgcggaac tgacgccgcc gctggccacg accggcatcg
     3841 tcgcgagcag gagtttcacc ctgaagaatc ctgaaaccct tgagaacatc atgaaggcat
     3901 tgatcgaggg tctggtcttc gtcctggctc ccggcaacaa atcacaggta atcaaaacgc
     3961 tcatgaaccg attcaagata tccgatgcgt cgctcgtcga gcccgaatac gccgatgccc
     4021 tgaaagacct ggagcccaaa ccttatccca cggcggaggg tgtcagaaat atgcaacgac
     4081 tcatgcaact gcaaaatccc cggctcgcca atatcaaccc cgcgagcctc atcgatagca
     4141 ccatcgttcg caaactggat gaaagcggct tcatccgcca gcttcaagca cgctacgggg
     4201 agc
//
LOCUS       DASQKS010000054         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5488342, whole genome shotgun sequence.
ACCESSION   DASQKS010000054 DASQKS010000000
VERSION     DASQKS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5488342"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VE170_01420"
     CDS             complement(<1..1560)
                     /locus_tag="VE170_01420"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ14115.1"
                     /translation="MPYFDLHQHLKNLEAHGLLRRITRPINKDTELHPLVRWQYRGLA
                     EEERRAWLFENVTDAKGRKYDMPVVVGALAGKPEIYYLGMGCSSGEEMDAMWKKALAE
                     PINPIAVTKAKCQEVVIQGDALDREGNGLDRFPVPISTPGFDNGPYTTCSHWITADLD
                     TGIQNIGNYRGEIKAGKRVGVFPSGLGQDIYLHWQKAVEKGVHLPAALVIGAPPIVSY
                     VSVQKIPYGVDEMGVAGALAGEPIPMVKCKTHDIMVPADAEIVIEGYLNTEFIEPEGP
                     FGESHGYMHPRQLNPFMDVTAITYRKDAIYTSWLSQLTPSESSVIRKIGYDRLMLVHL
                     RDECRITSVLRVEMHEPLTNTYKLVIIQMRKPNEAEVWRALHAACSFHPGVGKIVVAV
                     DEDIDPSNEAAVFWAMCYRMKPHKDVHIVPGMEKGHAPPFLYEHEVKGTDVVSYHLPA
                     DESAMLCNAILKEPFPPVSLPKREYMENARKIWEELELPKLRPQMPWFGYSLGQWSDE
                     LDHEAQLAVEGR"
     gene            complement(1895..2869)
                     /locus_tag="VE170_01425"
     CDS             complement(1895..2869)
                     /locus_tag="VE170_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14116.1"
                     /translation="MTICLFVAGKPWLRAAEPTKVLIAHGAISNNVEPLWIAKEQGIF
                     RKYGIDAELVFIIAGRAMQAMLAGQVPVGLVGGTHVVNANTGGGDFTMILGLENRLDY
                     LFLAKSTIKSGEDLKGKKVGIGTPAGSASLAAYVSMDHLGLVPRRDNIVLLGVGGVPE
                     RMGALRSGSIDATTLSPEIGQQVLGEGFRVLLDLGKENVQFQSSGLVFSRSWMKSNPQ
                     LVENIARATVEGTAYIHKPANKEIVLKSLGRNLRLTKPDQMEKAYQGLIGALPKRPCP
                     SIEGMNAVVKLMVQHGINTKAAQLKAEEQLDMSFCKRFEDSGFFKSLY"
BASE COUNT          644 a          902 c          788 g          680 t
ORIGIN      
        1 gcggccttcg acggccagct gcgcttcgtg atcgagctca tcgctccact ggccgagcga
       61 gtaaccaaac catggcatct gtggacgcag cttgggcagt tccagctctt cccatatctt
      121 gcgcgcgttc tccatatact cgcgcttggg cagcgacacc ggagggaacg gttccttcaa
      181 gatcgcattg cacagcatcg ccgactcatc cgccggcaaa tggtacgaca ccacgtcggt
      241 acccttgacc tcatgctcgt acagaaacgg cggcgcatgg cccttttcca tgccgggaac
      301 gatgtgaaca tccttgtgcg gtttcatgcg gtagcacatc gcccagaaaa ccgcggcttc
      361 gttggacgga tcaatgtcct cgtcgacggc gacgacgatc ttgccgacgc cgggatggaa
      421 tgaacaggcg gcgtggagcg cgcgccacac ttccgcttca ttgggcttgc gcatttgaat
      481 gatcaccagc ttgtaggtgt tggtcagcgg ttcgtgcatt tcgacccgca agaccgaagt
      541 aatgcggcac tcatcgcgca ggtgcaccag catcaagcgg tcgtaaccga tttttcgaat
      601 caccgatgat tcgctcggcg tcaattgact caaccacgaa gtatagatcg cgtcctttcg
      661 ataagtaatc gcggtcacgt ccatgaacgg attcaactgc cgcggatgca tgtagccgtg
      721 cgattcgccg aacggccctt cgggctctat gaactcggta ttcaaataac cttcgatgac
      781 gatctcggca tccgccggca ccatgatgtc gtgggtttta catttgacca tcgggattgg
      841 ttcgccggcg agagcgccgg ccacgcccat ctcatcgacg ccgtagggga tcttctgcac
      901 cgaaacgtac gacacgatgg gcggcgcgcc gatgaccagc gccgccggca aatgcacgcc
      961 cttctcgacc gccttctgcc aatgcagata aatatcctgg cccaaacccg aggggaagac
     1021 accgacgcgc ttgccggctt tgatctcgcc gcggtagttg ccgatgtttt gaatcccggt
     1081 gtcgagatcg gcagtgatcc agtgcgagca agtcgtgtag ggtccgttgt cgaatcccgg
     1141 cgtcgagatc ggcacgggaa agcggtcgag accgttgccc tctctgtcga gcgcatcgcc
     1201 ttggatgacg acttcctggc acttggcttt ggtcacggcg atgggattga tcggctcggc
     1261 gagagctttt ttccacatcg catccatttc ttcgccgctg ctgcagccca taccgagata
     1321 atagatctcc ggctttcccg ccaaagcgcc gaccaccacg ggcatgtcgt acttgcgtcc
     1381 cttggcatcg gtgacgttct caaagagcca ggcccggcgc tcttcctccg ccaatccacg
     1441 gtactgccaa cgcacgagcg gatgaagttc cgtgtccttg ttgatcggcc gcgtaatgcg
     1501 cctgagcagt ccatgcgctt cgagattctt cagatgctga tgtaaatcga aatacggcat
     1561 acggtgaatg tctcctcttc aaacaaacaa tctgaatgga atatccgatc gatctcggca
     1621 ggtcaacaat cagagagcaa gggatgaatc gcaagaaata gggagatgcg gttccctaac
     1681 cccgaatatc ttacttatta ctgcttacta tgacttgcct tacttgccgg cgagttggtt
     1741 cgaggactcg tcgcgcggta gcgctcactg aggaggattt atcgacgagt cgacggcgcg
     1801 cttgccgcga aactttttgt aagacagcaa atcgtcactg ataaaatgtc gtcgaatggg
     1861 acgatgcacg actgcattac acgagtcgcc ccgtttagta caaacttttg aaaaatccgc
     1921 tgtcttcgaa tcttttacaa aagctcatat ccaactgctc ctcggctttc agctgcgccg
     1981 ctttggtgtt gatgccatgc tgcaccatca gcttcaccac cgcgttcatg ccttcgatgg
     2041 atggacaagg acgtttcggc aatgcgccga tcaaaccctg gtaagccttt tccatttgat
     2101 cgggcttggt caagcgcaga ttacgcccga ggcttttaag cacgatctct ttattggcgg
     2161 gcttgtgaat atacgcagtt ccttcaaccg tcgccctggc aatattttcg accaattgcg
     2221 ggttactctt catccagctg cgggaaaaga ccagtcccga ggattggaac tgcacgttct
     2281 ctttccccaa atcgagcaac acacggaaac cttcgcccaa cacctgttgg ccgatctccg
     2341 gagatagggt ggtcgcgtcg atactgccgg aacgtaacgc acccatgcgc tcgggaactc
     2401 cgccgacgcc gagcagcaca atgttgtcgc gccgcggcac caggccgaga tgatccatag
     2461 aaacatacgc cgctaaagac gccgaacccg ccggcgtgcc gatgccgact tttttacctt
     2521 tgagatcttc gccgctcttg atggttgatt tcgccagaaa caaataatca agccggttct
     2581 ccaagccgag aatcatcgtg aaatcgccac cgccggtgtt ggcgttgacg acatgagtgc
     2641 cgccgacgag tccgacggga acctgtccgg ccaacatcgc ctgcatcgcc cggccggcga
     2701 tgataaacac cagctctgca tcgatcccgt acttgcgaaa aatcccctgc tccttggcga
     2761 tccacagcgg ctccacgtta ttgctgatcg ccccatgggc gattaaaact ttcgtcggct
     2821 cggctgcgcg caaccaaggt tttcccgcga cgaacaaaca aatggtcaaa aacattatcc
     2881 cggctcggtt tctcatctcg cctcgcttgt ttcctgccta cttaacggta tcggccgcgc
     2941 caacacagtc gccggtatta tcagaccgag ccgcttggcc gctggcaaat tcttagcgaa
     3001 ttcgaacttg atcg
//
LOCUS       DASQKS010000055         7502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2968189, whole genome shotgun sequence.
ACCESSION   DASQKS010000055 DASQKS010000000
VERSION     DASQKS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968189"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VE170_01430"
     CDS             complement(<1..306)
                     /locus_tag="VE170_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HYJ14117.1"
                     /translation="MATDATGMTRINHEKLIRFVSRSFEKLGVPPSDAEIAANVLVAS
                     DLRGVDTHGVIRFNPNAWYVKWLRDGAMTAKPNIRVVTETASNALLDADNGMGMVAGY
                     "
     gene            454..1488
                     /locus_tag="VE170_01435"
     CDS             454..1488
                     /locus_tag="VE170_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14118.1"
                     /translation="MMKFFDLPSRLILVAVCFARIAVVGVADIGAAELPLVRIAHGAF
                     SEKVVALWLGAEQGLFRKHGVNVEVINIRSGPQTMAALVSGDIQVAYTIPGSVVSAAT
                     SGLDVAFFAGIVNKADGDFIAGTSIRSAEDLKGKRIGVQSIGGGVWSMAMLAIEHLGL
                     EPNRDKMTIMVIGDSPVLAQSLESGGIEATYLNYTHSRALKEKGLPVLLDLGKAPIPY
                     QGLAAATRRGYLKQNPQIVDALMRGFVETVAFIHRPGNKEIVIKSLVKNLRLKSPQDA
                     EIGYGALQWLYNLDIKPTLPGIQNMARFLALSNPKVKSVKSEDVVDDAPWQRLEKSPF
                     YREVLAAAKK"
     gene            complement(1534..2535)
                     /locus_tag="VE170_01440"
     CDS             complement(1534..2535)
                     /locus_tag="VE170_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14119.1"
                     /translation="MKVKTLLLLAIFLPTIASAQSPELVNQAKKEGGEVILYTTMTVG
                     DFEHFSKAFKEKYPGLNLRHVYLSSSRQTARVMQEFRAGRVQGDVLGNSPEPLLYLKQ
                     QGVLGQYRSAETKNLLSGAWDNDGFWSGITTDLLVTGFNPRLLAKSAVPKNYDDYLRP
                     QYKSQIAVNRGVPYPLTGMVSLRGDEQGTAYFKKLSQQDLRLVEGYSHTINMMAAGEY
                     PLTGFMQVSKLEAMKRKEAPVDWLPATQTLATISTIAMVKNPLHPAAAQILIDFYLSP
                     EGQRALATAGKIPLRKGIRSQSKDIDQLMESGTAHVIRAEGSYDKYMKVYNEYLGIR"
     gene            complement(2555..3547)
                     /locus_tag="VE170_01445"
     CDS             complement(2555..3547)
                     /locus_tag="VE170_01445"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14120.1"
                     /translation="MARVGKQHRFLPAIIALSLSFWLNPLGAQEHSLRAVYNAISGVM
                     APIWVAADAGLFTKHGINVDVKYLAATSAVQAMLGGGEEVGFVGNQGIDAKLEGADLV
                     YIASGLPTFVFQLYSRPEIKTIADLKGKVIAVTQPAASTDYATRMVLRKNGLDPEKDV
                     KILYAQDSINVINTVITGNAAAGINSHPNALKLKAAGMKMLVDITELKIPFLFTGILS
                     SPKVVREKNEALTRFLRGYIEAMAVIRRDKEITVKSMGKFLKLSDREALESTYDEYKS
                     VFPVTPLMTAAEVQAVLDVSKSPKAKSMKPQEFFDNSIVQKIAASGFIEQVNRR"
     gene            complement(3626..5467)
                     /locus_tag="VE170_01450"
     CDS             complement(3626..5467)
                     /locus_tag="VE170_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HYJ14121.1"
                     /translation="MSEVKPVAGGPAQDVYSPAVVMLSGGMFLLVCAVVAAILLWIGR
                     SFALPASLLAAEVFATILGLFFFGSFKYQIHKNALTYGMLLIIVATFCGLSSSVWHID
                     ISSNGWWAWIAQNLLSFHGLDDLIHADTMLFILGLTFFVSVIAQTRLLEGLTFYLLRK
                     FRGAVLPTVIAVTAVVAFASGILDGVSMIGLTIRTLVIIMLLAAAPIAEVRYSVMVCT
                     TVTTVCGIWLAYGEPPNLIMKANLYPHIDNAFFLRYCAPAAVACYLVIAWNFSKRLRG
                     DQIELDKMDVIDANAEDVRFLQAMRHGKVATSLELVEDYENELGDKADVVRERLREGE
                     SLGIALLHAGVPDALRKKLLGHFVSDELAETLDRHYMLDAAGDHHGALEAERAVDDEL
                     AATGRQRQVAQKFGALALVPFIGMLIWHGIDHAVPLFLASFAGFGVALLGIIKLTKMR
                     NLALRDAKHEFAEYYFLFPLFVSITLLTKAGFFDHLQSLIQQGIQTLGIAHLAFVKFL
                     GVTFLSAILDNNVVADFASRTLHNLDISILHLFAMAQIAGYALGGCWTHIGSAQSVVA
                     FAFIQRDIDDRYTPVQWIKEITPVILEMMVVITVILYLESALLDWLN"
     gene            complement(5544..6089)
                     /gene="msrA"
                     /locus_tag="VE170_01455"
     CDS             complement(5544..6089)
                     /gene="msrA"
                     /locus_tag="VE170_01455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004957772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYJ14122.1"
                     /translation="MSNQVAVFGGGCFWCTEAVFDELRGVMSVVSGYAGGRNKNPSYE
                     QVCMGNTGHAEVIKIEFDPAQVSFRDLMTVFFATHDPTTLNRQGNDVGTQYRSAVFYA
                     DDKQKEEAAAFIKELDETKTFKNPIVTTLEPLTEFYPAEDYHQKFYANNPSQGYCQYM
                     IPPKITKLHKQFKELLKSQAS"
     gene            complement(6086..7048)
                     /locus_tag="VE170_01460"
     CDS             complement(6086..7048)
                     /locus_tag="VE170_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14123.1"
                     /translation="MVKFTLLSIALLGLLAPELTWAQAPPQKVVITYSSRSIASIDLF
                     VAQERGFFREEGLDAQLVQVRATAAIAALVSGEVHALGSVGSVIRAIPRGAPVKVLAI
                     SLRRPVFWLVSRPELKTFAELKGKVMGTTTLGGSQHTAGIRMLRRAGLNPDKDVTVVL
                     GGDVPTQLQALVNGSIQVGILSPPIVIVARDKYKMNILASAIDEFSSLQNGLAVLDKN
                     VRDPLMKKILRARAKGNRYFHQNERGTAEILAKYLNVDINTALETHRLSRGAFTTNGI
                     PGDAEIAEHLKGDAEILGLTAPIAPAKIFDFTLQREVNQELGGK"
BASE COUNT         1700 a         2190 c         1983 g         1629 t
ORIGIN      
        1 atagccggcg accatgccca tgccgttgtc ggcatcgagc aatgcattgg aagccgtctc
       61 ggtaacgacg cggatattcg gcttcgccgt catcgcgcca tcgcgcagcc acttcacata
      121 ccaagcgttg gggttgaagc gaatcacgcc gtgggtatcg acaccgcgca aatcggacgc
      181 caccaggaca ttggcggcaa tctcggcgtc actcggcggc accccaagtt tttcgaatga
      241 gcggctgaca aagcgaatca gcttctcatg attgatacgg gtcatgccgg ttgcatcagt
      301 ggccatgaat cctccaggag attacttgcg cacacctatc tttgatcttg ttcctctgtc
      361 aaccccgctt gacgctccta cgcccattcc ctatgttacg caaagccgaa aggtttcgac
      421 tgggtcacgc tcccacaccc gaggagattt ctgatgatga aatttttcga tttgccatcg
      481 cgtctgattc tcgtcgcggt ctgcttcgct cggatcgccg tcgtaggcgt cgccgatatc
      541 ggcgcggcgg aactgccgct ggtgcgcatc gcccacggcg cgtttagcga aaaagtcgtc
      601 gcactatggc tgggcgccga acaagggcta tttcgcaagc atggcgtcaa cgtcgaagtc
      661 atcaacatcc gcagcggccc gcagaccatg gcggcgctgg tttccggcga catccaggtc
      721 gcttacacga ttcctggatc ggtcgtcagc gcggccacca gcggtctaga cgtcgcgttc
      781 ttcgccggca tcgtcaataa agccgacggc gatttcattg ccggcacgag cattcgcagt
      841 gccgaggatt taaagggaaa acgtatcggt gtgcagagca tcggcggcgg ggtctggtcg
      901 atggcgatgc tcgccatcga acatctcggt ttggaaccga atcgagataa aatgacgatc
      961 atggttatcg gcgactcgcc ggtgttggcg cagtcgctgg aatccggcgg catcgaagca
     1021 acctatctca attacaccca tagccgggca ctgaaagaga aagggctgcc ggttctgctc
     1081 gacctgggca aggcgccgat accctatcaa ggactcgccg cggcgacccg gcgcggctat
     1141 ctcaagcaaa atccccagat cgtcgatgcc ctgatgcgcg gctttgtcga gaccgtggcc
     1201 tttattcacc ggccgggaaa caaggaaatc gttatcaaat cactggtcaa aaatctccgc
     1261 ttgaaaagcc cccaggacgc cgagatcggc tatggggcgc tgcaatggtt gtacaatctc
     1321 gatatcaagc caaccctccc cggcattcaa aatatggcgc gctttctcgc cctgtccaat
     1381 cccaaagtaa aatctgtgaa aagcgaagac gtggtcgacg acgcaccgtg gcaacgtttg
     1441 gaaaaaagcc cgttctaccg ggaagtgctg gccgctgcca aaaaatagcg cgtcgtcgta
     1501 gccgacctat agacagaact aactattgcg ggtctatctg atccctagat actcgttgta
     1561 aaccttcatg tacttgtcgt agctgccctc agcgcggatg acatgtgcgg tgccgctttc
     1621 catgagctga tcgatatcct tggactggct tctgatccct tttctgaggg gaatttttcc
     1681 ggccgttgcc aatgcgcgtt gcccttcagg ggagagataa aaatcaatca ggatctgcgc
     1741 cgccgcagga tgcaggggat ttttcaccat ggctatcgtg gaaatagtcg ccagggtctg
     1801 ggtcgcgggt aaccaatcca ccggcgcctc tttgcgtttc atcgcttcga gtttggagac
     1861 ctgcatgaac ccggtgagcg ggtattcgcc cgcagccatc atgttgatcg tgtggctgta
     1921 gccttcgact aaccttaagt cttgctggct gagttttttg aaataagccg tgccctgctc
     1981 gtcaccgcgc agcgatacca taccggtcag tgggtagggc acaccgcgat taacggcgat
     2041 ttgactcttg tactgcggcc gcaagtaatc gtcatagttt tttggcaccg cggatttcgc
     2101 gagtaatcgg ggattgaagc cggtgacgag caaatcggtg gttataccgg accagaagcc
     2161 gtcattatcc cacgcgcctg acagcagatt cttggtttcc gccgagcgat actggcccag
     2221 caccccttgt tgcttaaggt aaagcagcgg ctcggggcta ttgcccaaaa catcgccctg
     2281 aacgcggccg gcgcgaaact cttgcatcac ccgggcggtt tgccgcgatg aactgagata
     2341 aacatgacgg agatttaggc cgggatactt ttccttgaag gctttgctga aatgctcgaa
     2401 gtcgcccacg gtcattgtcg tgtagagaat gacctcgccg ccttccttct tcgcctggtt
     2461 caccagctcg ggcgactgcg ccgaagcgat agtcggcaag aaaatggcca gcaacaacag
     2521 agttttcact ttcatcacga cgcccctttc ggcgctaacg cctattcacc tgttcgataa
     2581 aacccgacgc cgcgattttc tggacgatgg aattatcgaa aaactcctgc ggcttcatgg
     2641 atttcgcttt cgggctcttg gagacatcga gcaccgcttg cacttctgcc gcggtcatca
     2701 aaggcgtcac cggaaagacg ctcttatact cgtcgtaggt cgattccagc gcctcgcggt
     2761 ccgacaactt gagaaattta cccatcgact tcaccgtgat ttccttgtcg cgccggatca
     2821 ccgccatcgc ttcgatgtac ccgcgcagaa accgggtcaa cgcttcgttc ttctcgcgca
     2881 cgactttggg cgatgacaat attccggtga ataaaaaggg aatttttaac tcggtgatgt
     2941 cgaccagcat cttcatcccc gcggccttga gcttcaacgc attgggatga gagttaatgc
     3001 cggctgcggc attgccggtg atcacggtgt tgatgacatt gatgctgtct tgagcgtaga
     3061 gtattttcac gtctttttct gggtccaggc cgttttttct aagcaccatg cgtgtggcgt
     3121 agtccgtcga cgcggccggc tgggtgaccg cgatcacttt gcccttgaga tcggcgatgg
     3181 ttttaatctc cgggcgggaa tagagctgaa acacgaacgt cggcagaccg ctggcgatat
     3241 agaccaggtc ggcgccttct agttttgcat cgatgccctg attgccgacg aagccgactt
     3301 cctcgccgcc gccgagcatt gcctgcaccg ctgaggtcgc tgcaagatat ttcacgtcga
     3361 cgttgatccc atgcttggtg aacagtccag cgtccgccgc cacccagatc ggcgccatga
     3421 cgccgctgat ggcgttgtag accgcgcgca gcgaatgctc ctgggcgccc agcgggttca
     3481 gccaaaacga caatgacaag gcaatgatcg ccggtaaaaa ccggtgctgc tttccgaccc
     3541 gagccataag cacgctccct gcatttagtc ccgccaactt accaatgcta aacaagaatt
     3601 tctagtttat atttggattg cggaactagt tcagccagtc cagcagcgcg ctttcaagat
     3661 agagtatgac cgttatcacc accatcattt ccaggatcac cggggtaatc tccttgatcc
     3721 actgcaccgg cgtatagcgg tcatcgatat cgcgctgaat aaacgcgaaa gcgaccaccg
     3781 actgcgccga gccgatatgc gtccaacagc cgcccaaggc atatccagcg atctgcgcca
     3841 tggcgaacag gtgcaaaatt gaaatatcca aattgtgcag cgttcgcgag gcaaagtcgg
     3901 cgacgacgtt attgtccaag atcgcggaaa gaaaggtgac gcccaggaat tttacaaaag
     3961 cgagatgcgc aattccgagt gtttggatcc cttgctggat taaactttgc agatgatcga
     4021 agaaacccgc cttggtcaac aaggtaatcg acacaaacag cggaaacaga aaatagtatt
     4081 cggcaaattc gtgcttcgcg tcacgcagcg ccaagttgcg catcttggtt aacttgataa
     4141 tccccagtag cgccacaccg aacccggcaa aagatgccaa aaacagcggc acggcatgat
     4201 cgatgccatg ccagatgagc ataccgatga acggtaccag agccaaagcg ccaaacttct
     4261 gcgccacttg gcgttggcgc cccgtggcgg caagctcgtc gtcaacggct ctttcggctt
     4321 cgagcgcgcc gtgatggtcg ccggcggcgt caagcatata gtggcgatcc aatgtttcgg
     4381 ctaactcgtc gctgacaaaa tgaccgagca gtttcttgcg cagcgcatca ggaacccccg
     4441 cgtggagcag cgcgatgccc agagactcac cctcgcgtaa gcgctcccgc acgacatccg
     4501 ccttgtcccc cagctcgttc tcgtaatctt cgacaagttc gagggaagta gccactttgc
     4561 cgtggcgcat cgcttggaga aagcgcacgt cttcggcgtt tgcatcgatg acatccatct
     4621 tgtccagttc aatctggtcg ccgcgcagtc tcttgctaaa attccaggca attaccagat
     4681 aacaggcgac cgccgccggc gcgcagtagc gcagaaaaaa agcgttgtcg atgtgcgggt
     4741 agagattggc cttcatgatc agattgggcg gctcgccgta ggccagccaa ataccgcaaa
     4801 ccgtcgtcac cgtggtgcag accatgaccg aatagcgcac ctccgctatc ggcgccgcgg
     4861 ccagcagcat gatgatgacc agggtgcgaa tggtcagacc gatcatcgac accccgtcga
     4921 ggatacccga ggcgaacgcc accaccgcgg tcaccgcgat cacggtcggc agcaccgcgc
     4981 cgcgaaactt gcgcagcaaa taaaatgtca gaccttcaag caaacgggtc tgcgcaatga
     5041 ccgagacgaa gaacgtcaag ccgagaataa agagcatggt atcggcatgg atgagatcgt
     5101 ctaaaccgtg gaacgatagt aaattctgcg cgatccaggc ccaccaacca ttcgaactga
     5161 tatcgatatg ccagacggaa ctgctcaagc cgcaaaacgt cgcgacgatg atcagcagca
     5221 tgccgtaggt taaagcattc ttgtgaatct gatatttgaa cgaaccgaag aaaaacaaac
     5281 cgaggatcgt cgcgaaaact tccgcagcca acagcgaggc cggcagtgca aatgaccggc
     5341 cgatccataa aaggatcgcc gcaaccacag cacaaaccaa caagaacatc ccgcccgaga
     5401 gcatgacgac agccgggcta tagacatctt gggccgggcc gcccgccaca ggtttcactt
     5461 cactcataaa gcgatatttc gaatcgaaag cgacggctga tgaatgcacc cgtcgcaagt
     5521 tcgccgccgc tgcattccgt ttctcacgag gcctgcgatt ttagcagctc tttgaactgc
     5581 ttgtgcagct tggttatttt cggcgggatc atgtactggc aatagccctg cgacggattg
     5641 ttggcgtaaa atttctggtg atagtcctcg gcgggataga attccgtgag cggctccaag
     5701 gtggtcacga tgggattctt gaacgtcttg gtctcgtcca gctctttgat aaacgccgcg
     5761 gcttcttcct tctgtttatc gtcggcgtag aaaaccgccg atcggtattg ggtgccgaca
     5821 tcgttgccct ggcggtttaa cgtcgttgga tcgtgggtgg cgaagaacac cgtcatcaaa
     5881 tcgcgaaaag aaacctgtgc cggatcgaac tcgatcttga tcacctcggc atggccggtg
     5941 ttacccatgc acacctgctc ataactgggg ttcttattcc tgccgccggc atagcccgag
     6001 actacggaca taacgccgcg cagctcgtcg aacaccgctt cggtgcacca gaaacagccg
     6061 ccgccaaaga ctgctacttg attactcatt ttccccccag ttcttggtta acctcacgct
     6121 gcaaggtgaa gtcgaatatc tttgccggcg cgatcggcgc cgtcaaaccg agaatttccg
     6181 cgtcgccttt aaggtgttcg gcaatctccg catcgccggg aatgccgttg gtcgtaaacg
     6241 ccccacgcga caacctatgg gtttccagcg cggtgttgat atccacgttc aaatatttcg
     6301 ccaaaatctc cgcagtgcct cgctcgttct gatgaaagta acggtttcct ttggcccgtg
     6361 cccggagaat cttcttcatc aatggatcgc ggacgttttt atccagcacc gccaggccat
     6421 tttgcagact gctgaattcg tcgatggcgc tggccaagat gttcatctta tatttatcac
     6481 gcgcgacgat gacaatcggc ggcgaaagaa taccgacctg aatcgagcca ttgaccaacg
     6541 cctgcaattg cgtcggcacg tcgcccccca acaccacggt gacatccttg tccggattga
     6601 gcccggcgcg ccgcagcatg cgaatccctg ccgtatgctg gctcccgccc agggtagtcg
     6661 ttcccatcac tttgcccttg agctccgcaa aggtcttaag ttcgggccgg ctcaccagcc
     6721 aaaataccgg ccggcgtaaa ctgatcgcca gcactttgac cggcgcacca cgcggaatcg
     6781 cccggatcac gctgcccacc gagcccagcg catgcacctc gccggagacc agcgcagcga
     6841 tcgccgccgt tgctcgcacc tgaaccagct gcgcatcgag cccctcttcg cgaaaaaaac
     6901 ctcgttcctg ggcaacaaat agatcgatgg acgcgatgct gcggctagaa taggtgatca
     6961 ccaccttttg tggcggtgcc tgtgcccagg tgagctccgg cgccaataag cccaagagcg
     7021 caatcgataa taaggtgaat ttcaccatgc ggtgatattc gcttgcggcc cgccgaagtt
     7081 caagcctatt ggcgaatata cacagctcga gctccgcaac tacaaataaa ccatggacac
     7141 ttggccggac ttcccggatg agtgccgacc aaggaacccc cgtcggcttc ggcaaaccag
     7201 cgatctcctt gccgcattgg cagcatttta tctttccctg cacatcacgc cgtttttccg
     7261 acatcttgat ataaagtcat ggtccgtcgg cgccgctgaa gtcccaaagc aatatttttg
     7321 tgattcgcgg ccgactttgt cgattgccag gtgccccgct gcgaattgtt gacttcaccc
     7381 tatgaacatg atttttctct aggttctgac agaagctcta ggaggatccg tatgggacaa
     7441 atgctcatag ccagcgagtt agtcgcctca gaaagcaaag aaaccactac catcaagaac
     7501 cc
//
LOCUS       DASQKS010000056         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4648219, whole genome shotgun sequence.
ACCESSION   DASQKS010000056 DASQKS010000000
VERSION     DASQKS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4648219"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VE170_01465"
     CDS             <1..911
                     /locus_tag="VE170_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14124.1"
                     /translation="NSRMAPLWLAEEQGYFAKYGMESQAVFLRSATVLVTGLGSGDIH
                     VGTGGGSAALAAVSSGYDVKVIGSFSGRNSYDFVARPNIKRAEDLRGKKVGVTSIGGG
                     SWMGALLWLEQLGLDPQRDQITLLAVGDQTVQSQALENGAVDATVLDGVFSRRLRQKG
                     LMILGEYSDLKQPMINQSVMVSSVLLQQKPEIAENFLRAMIESMAFTFAPKNRPAAVK
                     TIMRRLKIDQFSADEGYDDLLRGVEKKPFPSVDGLRNAQRLMKLRTPKIGELKVDTIV
                     DGRIMRKLEDSGFIDRVYAAHGAKAN"
     gene            965..1960
                     /locus_tag="VE170_01470"
     CDS             965..1960
                     /locus_tag="VE170_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14125.1"
                     /translation="MKPTIIFATMLCATLAFAKGGGAQQKLEKLHVGVAGLSGALAHA
                     FIPKDTGLYEKFGLDVDLVFFQGGTQGVQSLIAGGVQFIVSSGSEIIAARVAGGEITI
                     VAGYMNTVPYSIVADKGIRRFEDLKGSKAAVSRFGTTSDLALRYAFEQNGMSPGKDIT
                     IVQLGDQGARFAALTGGTVQSTVISPPFDLTAKKLGYRILADMADLGLHYQHEALATT
                     ERTLATRPDTASRFLKAFIAGIHVWMTDEKKTKEILARRLRIKDNEILDETYSAYKKL
                     TEKKPYPTVPGLEFQIADLALRNAKARGARADTFINLGLLKEIDKSGFIDALYKK"
     gene            2014..2589
                     /locus_tag="VE170_01475"
     CDS             2014..2589
                     /locus_tag="VE170_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ14126.1"
                     /translation="MAQVTKILVPIDFSPESGQALRDAAALARETWAQLIALHVIDEN
                     LERDMLLTSIVPFDSLPFPANGSATVPLDVLMRERALDLWNFVEREAEPPNRNRIKKV
                     VRLGKLTTEISNFMRDEQIDLLVLKLRRRRLFPDLTTLKLVTIAGRLSCPVLLEPPVP
                     RQRPDPRAGLLIFNLLAQVRLLCERPVITQS"
     gene            2828..>3055
                     /gene="nirA"
                     /locus_tag="VE170_01480"
     CDS             2828..>3055
                     /gene="nirA"
                     /locus_tag="VE170_01480"
                     /EC_number="1.7.7.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="ferredoxin-dependent assimilatory nitrite
                     reductase; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HYJ14127.1"
                     /translation="MNKIEEYKKEKAGLDILDDIPRYADAGWESITDGDKERLKWAGI
                     FFRRQTPGRFMMRLRFANGFINAAQLRTIGEI"
BASE COUNT          701 a          823 c          880 g          651 t
ORIGIN      
        1 tgaactcgcg tatggcgccg ctctggttgg ccgaggagca gggctacttt gccaagtacg
       61 gcatggagtc ccaggcagtg tttctgcgca gcgcgacggt gctcgtgacc gggctgggtt
      121 cgggagatat tcacgttggc acgggcggcg gcagcgccgc tttggcggcg gtgagcagcg
      181 gctatgacgt caaggtcatc ggcagtttct ccggccgcaa ttcctacgac ttcgtggcgc
      241 ggcccaacat caaacgtgct gaagatctgc gcggtaagaa agtcggcgtg accagcattg
      301 gcgggggctc atggatgggt gcgctgctct ggctggagca gcttggactc gatccccaac
      361 gcgaccaaat taccttactg gcggtgggcg accaaaccgt gcaaagccag gcgctcgaaa
      421 acggcgctgt cgatgccacg gtactcgacg gagtcttcag ccgccgtctg cgccagaaag
      481 ggttgatgat ccttggcgag tacagcgatc tcaaacagcc aatgatcaat cagtcggtga
      541 tggtatcgag cgtgttgctg caacaaaaac cggagatcgc cgagaacttt ctccgagcca
      601 tgatcgaaag catggccttc acgttcgcgc ccaagaatag acccgctgcg gtcaagacca
      661 tcatgcgccg actcaagatt gatcaattct ctgccgacga gggttatgac gacttgttgc
      721 gcggggtcga gaagaaacct tttccatccg tagatggatt gcgcaatgcg cagcgcttga
      781 tgaagctgcg tacgccgaaa atcggcgagc ttaaggtcga taccatcgtc gatggccgga
      841 tcatgcgcaa gcttgaagac agcggtttca tcgacagagt ttacgcggcg cacggagcca
      901 aagctaactg aattattcta tcgggcggga ggcgcgccaa cattgcgcag ttgaaaggat
      961 aacgatgaaa ccaacaataa tttttgcgac gatgctgtgc gcgacactgg ctttcgccaa
     1021 gggcggtggc gcacagcaaa aattggaaaa acttcatgtc ggcgtcgctg ggttgagcgg
     1081 cgcgctcgcc catgcgttca tccccaaaga caccggcctg tacgaaaaat tcggcctgga
     1141 tgtagattta gttttctttc agggcggcac ccagggggtg caatctctaa ttgccggcgg
     1201 cgtgcagttt atcgtgtcgt caggatcgga aatcattgcc gcccgggttg ccggcgggga
     1261 gatcacaatc gtcgccggct acatgaacac ggttccctat agcattgtcg cggacaaggg
     1321 gatccgccgg ttcgaagact taaagggcag caaggcggca gtcagccggt tcggcaccac
     1381 ctcagatctc gcgttacgtt atgccttcga acagaacgga atgtccccgg gcaaagatat
     1441 aaccatagtc cagttgggcg atcagggcgc tcgtttcgcc gcactcaccg gcggcacggt
     1501 gcaatcgacg gtgatctcgc cacccttcga tctcaccgcc aagaaactag gatatcggat
     1561 tctcgcggac atggcggatc tcggcttgca ctatcaacat gaagccttgg ccaccaccga
     1621 acgcacgctt gcgaccaggc ccgataccgc aagccggttt ctcaaggcgt ttatcgccgg
     1681 cattcatgtc tggatgaccg atgagaaaaa aaccaaagaa atactggcca gacggttacg
     1741 catcaaggat aacgagattc tcgatgaaac ttattcggcc tacaagaaac tgactgaaaa
     1801 aaaaccatat ccgactgtgc cgggcctgga attccaaatc gctgatttgg cgctgcgaaa
     1861 cgccaaagcc aggggcgcca gggccgatac gttcatcaat ctcggcttgc tcaaggagat
     1921 cgacaagagc ggttttatcg acgctctata caaaaaatag ccgtggttct gaccacgcgc
     1981 gccgatattc ctattttgct gaaagagatg aaaatggccc aagtaacaaa gattctagtc
     2041 cccatcgatt tttccccaga gtccgggcaa gccttgcgcg atgctgcagc actggcgcgg
     2101 gaaacttggg cgcagctgat cgcgttgcat gtcatcgatg aaaacctcga gcgggacatg
     2161 ttgctgacct cgatcgtgcc gttcgacagt ttgccgtttc ccgcaaatgg ttcggcaacg
     2221 gttccacttg acgttctgat gcgcgagcgg gcgctcgatc tgtggaactt tgtcgagcgt
     2281 gaggccgagc cgcctaatcg gaacaggatc aagaaagtgg tcagactcgg caagctcacc
     2341 acggaaataa gcaattttat gcgcgatgag caaatcgatc tgctcgtgct caaactacgc
     2401 cggaggcggc tgtttcccga tttaactaca ctaaaacttg tcaccatcgc cgggcggttg
     2461 tcatgtccgg ttttgttgga gccacctgtg cctcgacagc gccccgatcc gagagcggga
     2521 ttgctcattt tcaatctgct ggcgcaggta agacttttat gtgagcggcc ggtcataact
     2581 cagtcctaac aagcgattcg aagcagatgc gtttttccgg cgctcttcac tagttggatt
     2641 ggatcggctt ggcggtcccg aaacctccgc tgcatttgcg tcgtcatggc atgtgcctat
     2701 ttcgtaggcg gcaggcgcat gaatcgtaag cactatgatc ccggtgcggc attgagatcg
     2761 ttgaattcgt tggaccgtta gcgattcccg cggtggcata tccgttgctc atactgcttc
     2821 gtgggcaatg aataagatcg aagaatacaa aaaggagaaa gctggcctcg atattctcga
     2881 cgacattccg cgctacgccg acgcagggtg ggagagtatc accgacggtg acaaggagcg
     2941 tttaaaatgg gccggcattt tcttccgacg gcaaacgccg ggccgcttca tgatgcggct
     3001 gcgcttcgcc aacggcttta tcaacgcggc gcaactgcgc accatcggtg aaatc
//
LOCUS       DASQKS010000057         5237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_28462, whole genome shotgun sequence.
ACCESSION   DASQKS010000057 DASQKS010000000
VERSION     DASQKS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28462"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..483)
                     /locus_tag="VE170_01485"
     CDS             complement(226..483)
                     /locus_tag="VE170_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYJ14128.1"
                     /translation="MEFIGWIFFGLVVGVIGKLLMPGRDPGGFVVTILLGIAGALLGG
                     FIGRSLGLYREGDPVGFIMAVIGSIILLMIYRFAVGRRHTV"
     gene            complement(540..1004)
                     /locus_tag="VE170_01490"
     CDS             complement(540..1004)
                     /locus_tag="VE170_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HYJ14129.1"
                     /translation="MGATIAKHPIHPMLVVFPIGLWIFSLVCDLIYLFGAGNPLWRDM
                     AFYTMAGGWIGALAAAVPGLIDYFSMSPGATKRIATTHMTLNLIVVTLYAVNLWIRWS
                     ADGAGGGPIGLSVLAVLLMVIAGWLGGELVYVHGAEEKPQAARPQRDRQRVA"
     gene            complement(1030..3591)
                     /gene="ligD"
                     /locus_tag="VE170_01495"
     CDS             complement(1030..3591)
                     /gene="ligD"
                     /locus_tag="VE170_01495"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HYJ14130.1"
                     /translation="MTELAMALEIYRDKRNFRRTPEPRGVAGKSRGTSFVIHKHAARR
                     LHYDFRLEHDGVLISWAVPKGPSLDPGERRLAVKVEDHPLDYGAFEGNIPEGEYGAGA
                     VIIWDQGRWLPEGDPAAGLRKGHLRFRLEGKKLAGEWSLVRMGKAAKAGKENWLLIKG
                     RDRSATKKDILAGKPKSAVSGKTIEQIRGSGPAELKPANPEVRPARRAARSKNRKPKA
                     KTKSSASAKLPDFVELQLATLVDEVPNGDDWLHEVKLDGYRMLCRIDNGKVSFLTRSG
                     LDWTGRVSSLSQAAAGLPCQSALLDGEVVVLDDSGVSDFGALQDALSRHDTSRLIYFA
                     FDLLHLDGADLTAMPLTERKQRLSAVVKPDGSADGPIRYSHDVRGQGERLLGKACELG
                     VEGIVSKRAGGLYRPGRGREWLKIKCLARQEFVIIGFTDPAGSRAGFGALLLGYYSKD
                     KKLLYAGKVGTGFNADALTKLRRRLGELEQDDTPLAELPARGERRGAHWVKPALVAEV
                     AFTGWTRDGRLRHPAFAGLREDKAAVEIFREEPASTAVVAAQPNPLADVSGDTIAGIR
                     LTHPERVLYPEQGITKRDLALYYLEIADWVLPHLKDRPLTLVRCPESYDQECFYQRHA
                     NDMLDESVLRLRIKEKGRGHDYIAVDSLKGVIALVQAGVLELHTWGARRDRLDRPDRI
                     TFDLDPDPALPWSAVADAARQLRAHLADFGLGAFLKTTGGKGLHVVVPIARTIGWDET
                     KAFAKAMAHGMAADEPKKYLAVMSKAKRQGKIFIDYLRNGWAATAVAAFSTRARAGAP
                     VSAPLFWQELDSCSGDTFNLKNIGARLARLKSDPWAEYEAARRPLTAALRKTIVT"
     gene            complement(3588..4613)
                     /locus_tag="VE170_01500"
     CDS             complement(3588..4613)
                     /locus_tag="VE170_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYJ14131.1"
                     /translation="MSEARNKTASRYAFYADPLVQPWYTLCDYILGQLLDDPYDKGVS
                     MAKSATKKKPPRKTRRKTRERPSTRPIWKGAISFGLVNIPVAMFSAENEEKLSFRLLD
                     RRDLAPVRYRRVNEKTGKEVPWNEIVKGYEHEKDEYVVLNDSDFERANVEASQTIAIT
                     EFVNAKEISPVYFDKPYYLAPLKHGEKGYALLREVMRRTGKAGIARMVLRSREYLAAV
                     LVHGEVLVVNLLRFAHELRGSQPLDVPGKDLKKLKITDREVTMAKDLVETLEESWRPE
                     QFHEQYYDDLMKIIEAKIKSGKSKTIEQEHERPAAPKSSKVVDITKLLKRSLEQAQQP
                     ARRRKAG"
     gene            5042..>5237
                     /locus_tag="VE170_01505"
     CDS             5042..>5237
                     /locus_tag="VE170_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HYJ14132.1"
                     /translation="MAQIFHRSTNTLSRLSIFGAVIFAAGLLWLIAAVNRSAYVTESH
                     VARAQPVPFSHEHHVAGLGID"
BASE COUNT         1045 a         1579 c         1427 g         1186 t
ORIGIN      
        1 ttcacgcatg ttgattgagc cattatcgat ttcgaatgtt cttcctaatt cttcactcgg
       61 caggttgtgg agcaagcaga tcattttctt atcgcccgcg ttgtcgaaag tttttacacc
      121 gataattttt ggcggaccgg acacgcgctc cctatggatc cgcgtgtccg gccctatttg
      181 ccgcggcaat tcccggcgac tagccgccgg ggtttcatct tgcgactaaa ccgtatggcg
      241 tcgcccaacg gcgaaccggt agatcatcag taaaatgatc gaaccgatta ccgccatgat
      301 gaatcccacc ggatcacctt ctcggtacag gcctaacgac cgaccgataa acccgcctag
      361 taatgcgccc gcgataccga gcagaatcgt gactacgaat ccgccgggat cgcggcccgg
      421 catcagcagt tttccgatga caccgacgac caacccgaaa aatatccagc cgataaattc
      481 catgttccgt cctcctttat gaattataga gttgaaccca ttggcgtgca gcgctcagat
      541 tatgccaccc gttgccgatc gcgctgcggc cgggccgctt gaggcttttc ttcggccccg
      601 tggacgtaga ctagctcgcc gccgagccag ccggcgatca ccatcagcaa caccgccagc
      661 accgataacc caattggccc gccgccggcg ccgtccgctg accaacgaat ccacagattg
      721 acggcataga gcgtgacgac aatcaggttg agcgtcatgt gggtcgtagc aatgcgcttg
      781 gttgcgcccg ggctcatgct gaaatagtcg attaagccgg gcaccgccgc ggccagtgcg
      841 ccgatccagc cgcccgccat ggtgtagaac gccatgtcac gccacaaggg attaccggca
      901 ccgaagagat aaatcagatc gcacaccagc gagaaaatcc acagtccgat gggaaacacc
      961 acgagcatcg gatggatcgg atgtttggcg attgtcgctc ccataatgtt tcctccttgt
     1021 gttagagttt cacgtaacta ttgtttttcg cagcgccgcc gtcagcggcc ggcgcgccgc
     1081 ttcatattcg gcccaaggat cgcttttcaa tcgcgccagc cgcgcgccga tatttttcag
     1141 attgaaggta tcgccgctgc aactgtcgag ttcctgccag aataatggcg ccgacaccgg
     1201 cgcgccggcg cgcgcgcgcg tcgaaaaagc cgccaccgcg gtggcggccc agccattgcg
     1261 caaataatcg atgaaaattt ttccctgccg cttagccttt gacatcaccg ccagatattt
     1321 ttttggctca tcggctgcca tgccgtgggc catcgctttg gcgaatgctt ttgtttcgtc
     1381 ccagccgata gtgcgcgcga tcggcaccac cacgtgtagt cccttgccgc cggtggtttt
     1441 caaaaaagcg ccaagcccga aatccgccag gtgcgcgcgt agctgacgcg ccgcatcggc
     1501 caccgcgctc caaggcagcg ccggatcggg atcgagatcg aaggttatgc gatcgggacg
     1561 atcgagacgg tcgcgccgcg cgccccaggt gtgcagctcg agcacacccg cctgcaccag
     1621 cgcgatcacg cctttcaacg aatcgacggc gatatagtcg tgcccccgcc ctttttcctt
     1681 gatgcgcagc cggagcacag attcatccaa catgtcgttg gcatggcgct gatagaaaca
     1741 ttcctgatcg taactctccg gacagcgcac caaggtcaaa ggtcgatctt tcaaatgggg
     1801 caaaacccaa tcggcaattt ctaggtagta gagcgccaga tcgcgcttgg tgatcccttg
     1861 ttcgggatag agcacccgtt ccgggtgagt aaggcgaatg cccgcgatcg tgtcgccgct
     1921 cacatcggca agcgggttcg gttgcgcagc gacgaccgcc gtcgacgccg gctcttcgcg
     1981 aaaaatttcg accgccgctt tgtcctcgcg caatccggca aacgccggat ggcgcagccg
     2041 gccgtcgcgc gtccagccgg taaaggctac ttctgcgaca agcgcgggtt ttacccagtg
     2101 ggcgccgcgc cgttcgccac gcgccggcag ctcggccaac ggcgtgtcgt cttgctcgag
     2161 ctctcctaag cggcgccgca atttggtcag cgcatcggcg ttgaagccgg tgccgacttt
     2221 gccggcgtaa agcaatttct tgtccttgct ataatagccc aaaagcagcg cgccgaaacc
     2281 ggcgcggctg ccggcggggt cggtgaaacc gataatgacg aattcctggc gcgcgagaca
     2341 ttttatttta agccactcgc ggccgcgccc cggacggtat aaaccgccgg cccgtttcga
     2401 gacgatgcct tcgacgccga gctcacaggc tttcccgagc agccgttcgc cttggccgcg
     2461 cacatcatgg ctatagcgta tcggcccgtc ggcgctgccg tcgggcttaa caacggcgct
     2521 taagcgttgc ttgcgttcgg ttaatggcat ggctgtcaaa tctgccccat cgagatgcaa
     2581 tagatcgaaa gcaaaataga tcaagcggct cgtatcgtgg cggctgagcg cgtcttggag
     2641 cgcgccgaaa tcgctgacgc cggagtcatc gaggacgacc acctcgccgt caagcagagc
     2701 gctctggcac ggcaggccgg cggcggcttg gctcaaagac gaaacacgcc cggtccaatc
     2761 aaggccgctg cgggtgagaa atgaaacttt accgttgtcg atgcggcaca gcatgcgata
     2821 accgtccagc tttacttcat gcagccagtc atcgccattt ggcacttcgt cgaccagcgt
     2881 ggccaactgc aactcgacga agtcgggcag tttggcggag gccgagcttt ttgttttagc
     2941 tttgggcttg cggtttttcg accgcgcagc gcgccgcgcc gggcgaactt ctgggttcgc
     3001 aggctttagc tccgccgggc cactgccgcg aatttgttca atagtttttc ctgatactgc
     3061 gctcttcggt ttgccagcga gaatatcctt cttcgtggca ctacgatcgc ggcccttgat
     3121 caacagccaa ttttctttgc cggctttagc ggctttgccc atgcgcacga gcgaccactc
     3181 gccggctaac tttttccctt ccagacggaa gcgcaaatgg cccttgcgca agccggcggc
     3241 cgggtcgcct tcgggaagcc aacggccctg gtcccagata attaccgcgc cggcgccgta
     3301 ttcgccctcg gggatgttac cttcgaaggc gccgtaatcg agaggatggt cttcgacttt
     3361 gactgccaat cggcgctccc cgggatccag gcttgggcct ttggggaccg cccaactgat
     3421 gagcacgccg tcgtgctcca aacgaaaatc gtagtgcagc cggcgcgccg catgtttgtg
     3481 tatgacgaag ctcgtgccgc gactttttcc ggccacaccg cgcggctcgg gagtgcggcg
     3541 aaaattacgt ttgtcgcggt agatttcaag cgccatggcg agttcggtca cccggcttta
     3601 cgccgccgcg ccggctgttg cgcctgttct aagctgcgct tcaacagttt ggtgatatcg
     3661 accaccttgc tcgactttgg cgctgcggga cgttcgtgtt cctgctcgat cgtcttgctc
     3721 ttaccgcttt tgatttttgc ctcgatgatc ttcatcaaat cgtcgtaata ttgttcgtgg
     3781 aattgctcgg gacgccagga ttcttcgagc gtctcgacta gatccttagc catagtcact
     3841 tcgcggtcgg tgatttttag tttttttaga tctttgccgg gcacgtcgag gggttgcgac
     3901 ccgcgcagct catgggcgaa gcgcaaaaga ttcaccacta aaacttcgcc atgaaccaaa
     3961 acggcggcga gatattcgcg cgagcgtaac accattcgag cgatgccggc tttgcccgtg
     4021 cggcgcatga cttcccgcaa taacgcgtaa cctttttctc catgtttcaa tggcgcaaga
     4081 taataaggtt tatcaaaata aaccgggctg atttccttgg cgttgacaaa ctcggtgatg
     4141 gcaatggttt ggcttgcctc aacgttggcg cgctcaaaat cgctatcgtt gagcacgaca
     4201 tattcatctt tctcgtgctc ataacccttg acgatttcgt tccagggcac ttccttaccg
     4261 gtcttttcgt taacccgccg gtagcgcaca ggcgcaagat cgcgccggtc aagaagccga
     4321 aaactcagtt tttcttcgtt ttcagcggaa aacatcgcga caggaatatt aaccaggccg
     4381 aagctgatgg cgcctttcca gatcggccgc gtcgacggcc gctcgcgggt ctttcttcgt
     4441 gtctttcttg gtggcttttt tttggtagcg gatttcgcca tgctcactcc cttgtcgtac
     4501 gggtcgtcca atagctgacc taagatataa tcgcaaagcg tgtaccacgg ctgcacaagc
     4561 ggatcggcgt aaaaagcgta tcggctcgcc gttttgttgc gagcctcgga caatggctat
     4621 agtccccgtc tcaccgacgg gatatttatc gttcaacgcg gagcgagcga ctcaatgccg
     4681 tgtcccagga gcgaaacaaa aaattttgtt tttctcagtg ttgctaagag acaggaaaat
     4741 aaatttaccg cgatcgatgt cgatgaacac gatccgatag cgctgcgcaa tcgtgcaatg
     4801 attaaataat caagcctgca atcgccggct cgattgcaac atattgaaga tctacatttt
     4861 ttcggatgcc gacccggcat tttatttagc gagcacttcc agtggcgctg gcgcgtctag
     4921 tctaggttcc cccgaccggt acatcgttcc tcactggcac atacgttgct gacctcacct
     4981 aaggcgcggc gtttttcatg cgcgctagac ggaagtttgc ccgcaagttt gcaccgcttc
     5041 gatggctcaa atatttcatc gcagcaccaa taccctttcg cgcctgagta ttttcggcgc
     5101 ggtgattttt gccgcaggcc tactgtggtt gatcgccgcc gttaaccgat cagcctatgt
     5161 gaccgagagc cacgtcgccc gcgcccagcc ggtacccttc agccatgagc atcacgtcgc
     5221 cgggctgggc atcgact
//
LOCUS       DASQKS010000058         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5071854, whole genome shotgun sequence.
ACCESSION   DASQKS010000058 DASQKS010000000
VERSION     DASQKS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5071854"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VE170_01510"
     CDS             <1..613
                     /locus_tag="VE170_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14133.1"
                     /translation="NTLGVRAKGDLTHFYGVDRKSVTWITPGKEVVDVELPGDLKIET
                     RDSMAEIEKEFVDGKIQAMFVSRLPKPFREGNKNLARLFADPQAEEERYLREEGYFPI
                     MHVLAYKKDLAEKHPEAPRALFDIFEQARGRAMQRWVDPNWSMVMWGRKELERQIGLC
                     SFDPWKNGLEANRKNIERFALYSHEQGLTKRRLTPEELFVGIE"
     gene            complement(641..1933)
                     /locus_tag="VE170_01515"
     CDS             complement(641..1933)
                     /locus_tag="VE170_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14134.1"
                     /translation="MTEPAESRRPPRFFYGWYIVAVGILVNIAGTFAFSSTLSIFLKP
                     ITEELGVTRGAFSLIRTFEIGVAALIVPMLGSWIDRHGGRGVLVLGVLMEGAGLLLSS
                     LVQSFWQFVVVRCSLVIAGEALLGSLVINVTIAQWFVRKRGRAMGIANLGTGVAKLGI
                     PIAAASLFVLVGWRYTWAIFGIIAPLLVVAPALIFVRRQPEDLGLRPDGDAPLAPASS
                     RQSTDESPKESAQSSNETVWTRAQAIRLPTFWVLVITFGIASVGIAGLNLHIFSYVTD
                     IGHSPLVAASFMSTIAITQLGSTLVWGMLADRFDIRKVSCIQFLIQGVGLITAITSRQ
                     IYVTYIGFFLYGTGLAGSFVLREVIWANFFGRNSLGTVRGLSMLFSHLFAASGAPFFG
                     FLHDRTGSYDLSFTLFSCALFTSAFLVLLAKKPHSLLS"
     gene            complement(2094..>2625)
                     /locus_tag="VE170_01520"
     CDS             complement(2094..>2625)
                     /locus_tag="VE170_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14135.1"
                     /translation="GSYSMFFVEPGGNYWEIESYEHAVEHGMGKTTNPHWTKPLPKEA
                     FAGKGYVPQALTHGTLENDDLAASERFYRDALGLEVVKLWPSSCYVKHADTPWYIVCI
                     QAQNPNRPRLSRYQRFTVALESPTAVHDAHREFTANRESWQLRELETIRESSNAASFL
                     FADLNGNWWEISHAIA"
BASE COUNT          623 a          679 c          804 g          519 t
ORIGIN      
        1 gaatactcta ggcgtgcgcg ccaagggcga cctgacgcac ttttacggcg tcgatcgaaa
       61 atcggttact tggatcacgc cgggaaaaga ggtggtggat gtcgaattgc ccggcgattt
      121 aaaaattgaa acccgcgaca gcatggccga gatcgagaag gagtttgtcg acggaaagat
      181 tcaagcgatg ttcgtgtcgc gcctgcccaa gcccttccgc gaaggcaaca agaacttggc
      241 gcggttgttc gccgatccgc aagccgaaga ggagcgctat ttgcgcgagg aaggttactt
      301 cccgatcatg catgtgctgg cttacaaaaa agacttggcc gagaagcatc ccgaggcgcc
      361 gcgcgcgctt ttcgatattt tcgaacaagc ccgcggccgc gctatgcagc gctgggtcga
      421 tcccaactgg tccatggtga tgtggggcag gaaagagctg gagcggcaga tagggttgtg
      481 tagcttcgat ccgtggaaga acggcctaga agccaatcgc aagaacatcg aacgcttcgc
      541 gctctatagc catgagcagg gactgaccaa gcggcggctc acgccggagg aattatttgt
      601 cgggatcgaa tagctctatt gcggttttcc tgtgcgagtt ttaagacagc aggctatgcg
      661 gctttttcgc caacaaaacc aaaaatgccg aggtaaaaag ggcgcaggaa aagagcgtga
      721 acgagagatc gtaactgccg gtgcggtcat gcaggaaacc aaagaaaggc gcgccgctgg
      781 cggcgaataa atgcgaaaac aacatcgata gtccgcgcac ggtgcccaag gaattccgac
      841 cgaagaaatt agcccaaatc acttcgcgca atacaaagct ccccgccagg ccggtgccat
      901 aaagaaaaaa gccaatatag gtaacataaa tttgccggct agtgatggcg gtgatcaggc
      961 cgacgccctg aatcaggaac tggatgcaag acacctttcg gatgtcgaat cggtccgcga
     1021 gcatgcccca gaccagtgtg gaacctagtt gggttattgc gatggtgctc ataaacgacg
     1081 cggcgacgag cggggagtgg ccgatgtcgg tgacgtaaga gaatatatga agattcaatc
     1141 cggcgatgcc gacgctggcg atgccgaagg tgatgaccag tacccaaaac gtcggcaagc
     1201 gtatcgcttg agcgcgggtc cacacggttt cattcgagct ttgcgctgat tccttgggac
     1261 tttcatcggt gctctgccgg gacgaagcgg gcgccagcgg tgcatcgcca tctggtcgga
     1321 gacctaaatc ttcgggttgg cggcgcacga agatcagcgc gggggcgacc actagcagcg
     1381 gcgcgatgat gccgaagatc gcccaagtgt agcgccaacc gaccagcacg aacagcgacg
     1441 cggcggcgat cggaatgccc agcttggcga caccggtgcc gaggttggcg atgcccatgg
     1501 cgcggccgcg cttgcgcacg aaccattggg cgatggtgac gttgatcacc agcgaaccaa
     1561 gcagcgcttc gccggcgatg accaatgaac atcgcacgac gacgaactgc caaaaactct
     1621 gcaccaagct cgaaagcaat agccccgcgc cttccatgag cacgccaagg acgagcacgc
     1681 cgcgcccgcc atggcgatcg atccaagaac cgagcatggg cacgatcagc gcggcgacgc
     1741 cgatctcgaa tgtacgaatc agtgaaaacg ccccgcgcgt tacacccagt tcttcggtaa
     1801 tcggcttgag aaaaatgctc aacgtgctgg aaaaggcgaa agtgccggcg atgttgacga
     1861 gaatgccgac ggcgacgatg taccagccgt agaaaaaacg cggtggcctg cggctttccg
     1921 ctggttcagt caaattgtgc atgcgggtga aaacaattgt ttcgggcgag gatgtgtcgg
     1981 ttaattgtcc actgattagc agattaaata gctggagtgg gattttttaa tgtgcgagga
     2041 atgaatcgcc tcgatccccc ttttcaaacg ggatgcttaa ggtacatgcg tgactaggca
     2101 atggcgtgac tgatctccca ccagttgccg tttaagtcgg caaacaaaaa cgacgctgca
     2161 ttactcgatt ctcgtatcgt ttcaagttcg cgcagttgcc aactttcgcg attagcggtg
     2221 aattcacgat gagcgtcgtg caccgctgtc ggcgactcta atgcgacggt gaagcgttgg
     2281 tagcgcgaga gtctcggacg gttgggattt tgcgcttgga tgcagacgat gtaccagggc
     2341 gtgtcggcat gcttgacgta gcaggagctg ggccacagtt tgactacttc caaaccgagg
     2401 gcgtctctgt agaagcgttc ggatgcggcg aggtcgtcgt tctctaacgt tccgtgggtc
     2461 agcgcttggg gcacgtagcc tttgccggcg aatgcttctt tgggcagcgg ttttgtccag
     2521 tgcgggttgg tggttttgcc catgccgtgc tcgacggcgt gttcgtagga ctcgatttcc
     2581 caatagttgc cgcccggctc tacgaaaaac atggagtagg acccg
//
LOCUS       DASQKS010000059         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4652706, whole genome shotgun sequence.
ACCESSION   DASQKS010000059 DASQKS010000000
VERSION     DASQKS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4652706"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1330)
                     /locus_tag="VE170_01525"
     CDS             complement(35..1330)
                     /locus_tag="VE170_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14136.1"
                     /translation="MESTAELTRNPVKPKIFYGWYIVAVGFLANVASSFALASTMSIF
                     LKPLTADLGISRGVFSLLRSGEGIVAACIAPLIGTLVDRYGGRWLMVVGTGIVAVGYF
                     LLSHIDTFAQFTMIRLTLVTLGDSMMGYMVVNVIISQWFVRRRGRALAISSMGVGFAK
                     VCMPVVAASLILWLGWRQTWFVFGLMTLGLLVVPALLIIRRSPESMGLLPDGATEPAA
                     SAAAASQSAARVAETGAQDAIWTRAQATRTTAFWLLVITFGISSIGVTGLNLHVYSYV
                     TDSGYSAVVAATVMSIIASMQLASPLGWGFLAEKIGARYTATLRFVIQGIGLAVAILS
                     GNLLCLYGGFLLYGIGLGGNMVLPDMLWADYFGRQSLGKIRGLGLLISHFLAAIGPPF
                     FGFLFDITGGYGLSFAIFGAVLAISALLSLMLKPPRRNP"
     gene            complement(1507..2289)
                     /locus_tag="VE170_01530"
     CDS             complement(1507..2289)
                     /locus_tag="VE170_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ14137.1"
                     /translation="MKSFIGIFLLLLFTGCATQRDKLVWRVKDVVLSPYSEIMLEGPH
                     GEIALAVPTRTIQEMFLAHTRIIRTANVQSELYIAAGDDPNAFAGPDATGRNIIGINV
                     GMVKLVGADVSVYAALIGHEAAHLAKGHGASGKLRSNSIDLVATLVGAGLGMAGVPGG
                     GLISGFAADVIDSVYSRDQEREADALGVDYLLANRYDPQGAIRLHELLIKSSSGFRIP
                     FLASHPSSDERVENLRALIEAKKPKDESAAPIPPLDGAAAKQ"
     gene            2398..3120
                     /locus_tag="VE170_01535"
     CDS             2398..3120
                     /locus_tag="VE170_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYJ14138.1"
                     /translation="MKQLSIHIIILLALASAALAQQSPERRPRPPSISVNGEGLIAAE
                     PDQAQIDIGVTNQARNAPDASRENAERISRVLGEVKKLLGKGDEVKTSGYSLTPQYRY
                     PQGGKPEIVGFTANNIVRIKTAKLDEVGKLIDVAMQAGANNINRLAFTLKDEEGARLD
                     ALKQAAAKAKTKAEAMAASLGLKIVRIASVTEGERSFQPVFRAMPMARSEIAAAQAPT
                     PIEQGTVDVRSTVTLVAEVSER"
     gene            complement(3236..4681)
                     /locus_tag="VE170_01540"
     CDS             complement(3236..4681)
                     /locus_tag="VE170_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYJ14139.1"
                     /translation="MDIGIEKKFVFDTVEHNGAAIAQLCDNIFYFAELGMQEFETSGL
                     MMELLEKAGFAIERNLSGMPTGFVATYGSGKPVVALHTEYDATPGCSQQAGIIEPTPI
                     VDGAPGHTEGHNVNGAVMIGAAIAIKQAMDRFGLTGTLKVFGAPGEEQLVSRPYFVRD
                     GHFKDVDVALHDHIGSGFGTVYGLRQYALISAEFTFKGQSAHAATAPWNAKDALDAVV
                     LMDIGWDKLREHLEPTQRSHRVITNGGDQPNVIPNKAAIWWFFREATAEKTRALFEKA
                     KRIAEGAAIMTDTSYSVNVLSAVWPTRANRTLAEVIQANVEQIGMPQWAEGEQKLVCD
                     LQAKINKKAAGMPTQIMALKECVQSVSANDSGEVSWVVPTGLITFPSNIPGVSYHHWS
                     AGVSLATTIAHKGAVAGAKAMAASALDLFMDPKLVAKAKKTFNEEIGDTEYRPLLPPD
                     QKPPLELNRDLMERHRPAMRAHYLKEKPVFS"
     gene            complement(4787..>5124)
                     /locus_tag="VE170_01545"
     CDS             complement(4787..>5124)
                     /locus_tag="VE170_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HYJ14140.1"
                     /translation="MRVKSGRMVVVPYELGVNDIRVFQRENHTPEQYYRMVCDHFDTL
                     YKDSVSGGRVLCLPLHPFVIGMPFRIKYLEMALEYMCSHEGVWRTTGGEIAEWYYKNY
                     YKDPGRFDG"
BASE COUNT         1157 a         1560 c         1428 g          979 t
ORIGIN      
        1 aacggcttga actgcttgaa cgatttgaac gtgttcaagg gtttcgccgc ggcggcttca
       61 acatgagact gagcagcgct gaaatcgcca gcacggcgcc aaagatcgca aacgacagcc
      121 cgtaaccgcc ggtgatgtcg aagagaaagc cgaagaacgg cggaccgata gcggcgagaa
      181 aatgcgagat cagcaatcct aaaccgcgaa ttttgccgag agactggcgc ccaaaataat
      241 cggcccatag catgtccggc aacaccatgt tgccgcccag accgatgccg taaagcagaa
      301 agccgccgta taggcagagc aggttcccgc tcagtatcgc caccgccagt ccaatgccct
      361 ggatgacaaa gcgcaaggtc gcggtgtagc gggcgccgat cttctcggcg agaaatcccc
      421 agcccaacgg cgaagccagc tgcatcgatg cgatgatgct catcaccgtg gccgcgacca
      481 ctgccgagta acccgagtcg gtgacatagg aatagacatg cagattgagt ccggtgacac
      541 cgatgctgga aataccgaag gtgatcacca agagccagaa cgccgtcgtc cgcgtggctt
      601 gggcacgcgt ccaaatcgca tcctgcgcgc cggtctcagc cacgcgcgcg gcggactgag
      661 acgcagccgc agcactggcc gccggttcgg tcgcgccgtc gggtaacagt cccatcgatt
      721 ccggcgagcg ccggatgatt aagagcgcgg gcaccaccaa aagtcccaac gtcatcaagc
      781 cgaaaacgaa ccaggtctga cgccagccaa gccagagaat tagcgaagcc gccactaccg
      841 gcatgcaaac tttggcaaaa ccgacgccca tgctcgagat cgccagcgct cggccgcgcc
      901 ggcgcacgaa ccattgagag atgatgacat tgaccaccat gtatcccatc atcgagtcgc
      961 ccagggtcac caacgtcaag cggatcatcg tgaactgggc aaaggtatcg atatgactca
     1021 agagaaaata gcccaccgcg acgatgccgg tgccgacgac catcaaccac cgcccgccgt
     1081 agcgatcgac caacgtgccg attagcggcg cgatacatgc ggcaacgatg ccttcgccgg
     1141 agcgcagtaa ggaaaaaact ccgcgagaaa ttcccagatc ggcggtcagc ggcttgagaa
     1201 agatgctcat ggtgctcgcc agcgcaaagg aggaagcgac gttggcgaga aagccgacgg
     1261 caacgatgta ccaaccgtaa aaaatttttg gcttgacggg gttgcgggtt aactcagcag
     1321 tggattccac attcttggtt taaaggaaaa gcgccgcact agcaattccg gcctgcggcg
     1381 gaaagcgctg aattatcttt tgtggtcaat caaagattcg atggtctgtc atctcgaacg
     1441 aagtgagaga tctagcgttg agacgagatt cctcgccggc gctcggaatg acagaacgtt
     1501 gcgacgctat tgtttagcag cggcaccatc gagaggtgga atcggcgccg ccgactcgtc
     1561 tttcggtttc ttcgcttcga tcaatgcgcg gagattttcc acccgctcgt cgctgcttgg
     1621 gtggctggca agaaacggta tgcgaaagcc gctgctggac ttgatcagca actcatgcaa
     1681 acgaatcgcg ccctgaggat cgtatcgatt ggcgagcaga tagtcgacgc ccaacgcatc
     1741 cgcctcgcgt tcctgatcgc ggctgtacac cgaatcgatg acatcggcgg caaatccgct
     1801 gatcaagccg ccgccgggca cgccggccat gcccaaaccg gcgccgacaa gcgtagcgac
     1861 gaggtcgatg gagttggaac ggagctttcc cgacgcccca tgccccttgg ccagatgtgc
     1921 cgcttcatga ccgatcaacg ccgcgtatac gctgacatcg gcgccgacca acttcaccat
     1981 gccgacgttg atgcctatga tgttgcgccc ggtcgcatcc ggacctgcga acgcgttcgg
     2041 gtcatcgccg gcggcgatat agagttcgct ctgtacgttg gccgtccgaa tgatgcgcgt
     2101 atgggccaaa aacatctcct gtatcgttcg agtcggcacg gcaagggcaa tctccccgtg
     2161 cggaccttcc aacatgatct cagaataggg cgataggaca acgtccttta cccgccatac
     2221 gagcttatct cgttgggtag cgcagccggt aaagagtaaa agcaagaata ttccaatgaa
     2281 cgatttcatc gccgcctcgt ctaatttttt gaccatatcg cagtcgcggt tcaaagatag
     2341 cggccaggat actatcgcgg taggatcgaa tcatcgccca cggcgaaagg attgcacatg
     2401 aagcaactgt ctattcatat tatcatacta ctcgctttgg cgtccgcggc gttagcccag
     2461 caaagcccag agcggcgccc ccggccgccg tcgatatcgg tgaacggcga aggcctcatc
     2521 gccgccgaac ccgaccaggc acaaatcgat atcggcgtca ctaaccaagc gcgcaacgcg
     2581 ccggacgcat cgcgagaaaa cgccgagagg atttcccgcg tcctcggcga agtaaaaaag
     2641 cttctgggca aaggggacga agtgaaaacc agcggctact cgctcacgcc gcaataccgc
     2701 tatccccaag gcggcaagcc ggaaatcgtc ggcttcacgg cgaataatat cgtgcgcatc
     2761 aagaccgcca aactcgacga ggtcggcaag ctgatcgatg tcgccatgca ggccggcgcc
     2821 aataacatca accgactggc attcacatta aaggatgaag aaggcgcccg tcttgacgcc
     2881 ctcaagcaag ccgccgccaa agcgaaaacg aaagccgaag ccatggccgc gtcattggga
     2941 ctgaagatcg tcagaatcgc ttcggtgacc gaaggcgaac gttcgtttca accggttttt
     3001 cgagcgatgc ccatggcacg atccgaaata gcggctgccc aagcaccgac accgatcgag
     3061 cagggtactg tcgacgtcag atcgacggtg actttggtgg cggaagtaag tgagcggtga
     3121 cgctaggaca gtcttcttta gcgttaggcg tttccagact acagactcgg ctggccaacc
     3181 ttttggccgt catgctgcat agtgcaaagg ataacatcgc ttgaccaatc acccgctagc
     3241 taaacaccgg cttctctttt agataatgcg cgcgcatcgc cggccgatgg cgctccatta
     3301 aatcacggtt caattccaac ggcggtttct gatccggcgg cagcagcgga cggtattcgg
     3361 tgtcgccgat ctcttcatta aatgtctttt ttgccttggc gaccagtttg ggatccatga
     3421 atagatcgag ggccgacgcc gccatcgcct tggcgccggc gaccgcgccc ttatgcgcga
     3481 tagtcgtcgc caacgacacg ccggcggacc agtgatgata ggagacaccg ggaatgttcg
     3541 acgggaacgt aatcaagcct gtcggaacga cccaggatac ttcgcccgag tcattggcgg
     3601 agaccgactg cacgcattct ttcagcgcca ttatttgcgt cggcataccg gcggctttct
     3661 tgttgatctt ggcttgcaag tcgcaaacta gcttctgctc gccttcggcc cattgcggca
     3721 tgccgatctg ctcgacgttg gcttgaatca cctccgccag cgtgcgattc gcccgcgtcg
     3781 gccagacggc actcaggacg ttaaccgaat acgacgtgtc ggtcatgatc gcggcgccct
     3841 cggcgatgcg cttggctttc tcaaacagcg cacgggtttt ctccgccgtc gcttcgcgga
     3901 agaaccacca gatcgccgcc ttattgggaa tcacgttggg ctggtcgccg ccgttggtga
     3961 tcacccggtg gctgcgctgg gtcggctcca agtgctcgcg cagcttgtcc caaccgatgt
     4021 ccattaacac caccgcatcg agcgcgtctt tcgcattcca cggcgcagtc gccgcatggg
     4081 cggactgtcc cttgaaagta aactcggcgg agatcagcgc gtactggcgc aggccgtaga
     4141 ccgtgccgaa gccgctgccg atatgatcgt gcaatgccac gtcgacatcc ttgaaatggc
     4201 cgtcgcgcac gaagtagggc cggctcacca gttgctcttc gccgggcgcg ccgaacacct
     4261 tcagtgtgcc cgtaagcccg aaccgatcca tcgcctgctt gatcgcgata gcggctccga
     4321 tcatcaccgc gccgttgacg ttgtggcctt cggtatggcc cggcgcgccg tcgacgatcg
     4381 gcgtcggctc gatgatgcct gcctgctgcg agcaccccgg cgtggcgtcg tactccgtgt
     4441 gcaacgccac caccggtttg cccgacccgt aggtcgcgac gaacccggtc ggcatgccgg
     4501 atagatttcg ttcgatggcg aagcccgcct tctccaacag ctccatcatc aatccggaag
     4561 tctcaaactc ctgcatgccg agctcggcga aataaaaaat attgtcgcag agctgcgcga
     4621 tcgccgcgcc gttatgctcg acggtatcga agacaaactt cttttctatg ccaatatcca
     4681 taaacaacct ccgaagttac tctttgctgg agtattggag tggtggagta ttgagtttct
     4741 cggaatccga cccatcactc cagtactcct tcactccaac tcgtcttcac ccgtcaaacc
     4801 gccccggatc cttatagtag ttcttgtagt accactcggc gatctcccct ccggtggtgc
     4861 gccacacgcc ttcgtgcgaa cacatgtact ccagcgccat ttccaaatac ttgatccgga
     4921 acggcatgcc gatgacaaag gggtggagcg gtaagcacag cacgcggccg ccagacacgc
     4981 tgtctttgta cagcgtgtcg aagtgatcgc agaccatccg ataatattgc tccggggtgt
     5041 gattctcgcg ctggaaaacc cgaatgtcgt tgacgccaag ctcgtacggc accacgacca
     5101 tgcggccgct cttcacccgc atcg
//
LOCUS       DASQKS010000060         7896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4652860, whole genome shotgun sequence.
ACCESSION   DASQKS010000060 DASQKS010000000
VERSION     DASQKS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4652860"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VE170_01550"
     CDS             <1..199
                     /locus_tag="VE170_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14141.1"
                     /translation="LSGEACGFPFEQSKEYLVYVFAEPRDLQTGICTGTKSIAEAAQE
                     MEQLDEIVAAAKKSGATNVYR"
     gene            186..722
                     /locus_tag="VE170_01555"
     CDS             186..722
                     /locus_tag="VE170_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HYJ14142.1"
                     /translation="MFTDKQRGVIRGVVPAATATVVGFGGGSLLIPMSALAADEPAAR
                     IAWTLQWAVLPIFSLMIFIMRVANHRFSTPEDIDGSGLTSGTSRILVLRAILQNTLEQ
                     AVLAVAVYMIWAAVMPHSWLRAIPMAALLFVVGRILFASGYERGAAGRAMGFGLTAYP
                     TFGMLTTVATFLAWRVVR"
     gene            760..1653
                     /locus_tag="VE170_01560"
     CDS             760..1653
                     /locus_tag="VE170_01560"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ14143.1"
                     /translation="MATAGFHGVFPYLISPIDSAGAVKSDVLARLCDDLIDAGVHGLT
                     PLGSTGEFAYLNWAQRRRVVEVVVGAARGRVPVVAGVASTTIADATAQAREFAQMGAS
                     GILAILEAYFPLSDAGVFDYFKAIADAVSLPVVLYTNPTFQRSDLSLPVIDRLSHVAN
                     IGYIKDASNNTGRLLSILNQVQGRMKVFAASAHIPAAVMLIGGVGWMAGPACVAPRQS
                     VALYETCRCGNWTGAMELQRPLWALNQAFAKYNLAACIKGALELQGYAVGAPLPPQAP
                     LTAEALADVKQTLIALGALQH"
     gene            1741..2742
                     /locus_tag="VE170_01565"
     CDS             1741..2742
                     /locus_tag="VE170_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14144.1"
                     /translation="MMYRNRVHFAAGAMAAIWLVGCLGIALAATPAPHKRVRILFADF
                     SERMGLFFVAKDQRFFEEQGLDADLVQVNTGPVAVAAMAANEAEFYTVSATGSALGAI
                     SSGLDLVWVAGMINKLDGYFYVSPKVQKPEDLKGKTLGVQSIGGGIWMFVMMTLDHWG
                     LSPERDKIQMRVIGDQSVLVQSLNAGLIDGSVWGYAYNAALQRSGGRLLADLTALNIP
                     YQGTGLVARRAFIASSPDVVEKTLRGFVKANNFVRDKSNQAAVVRSIRKWLRLSPDGN
                     ADDLYERMRLLYDRRIAPTREGMQNALRVLGKVEPKYSKLKVEDLVDDRIARKLETP"
     gene            2791..4131
                     /locus_tag="VE170_01570"
     CDS             2791..4131
                     /locus_tag="VE170_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3850..3852,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HYJ14145.1"
                     /translation="MIRIVHILNQFFAGIGGEDKADIPVAVADGAAGAARGLQSQLAE
                     RGQIVATVYFGDNYFHEHKDDALAAITAELEKQKPDVVVAGPAFNAGRYGLACVEICQ
                     AVSEKLGLRCVTGMEPENPGVGVYRDNHNDKVFLLPTAETASGMARALAALATFACRL
                     ANGEEIGPAADEGYLPRGIRRLVRVDKSGADRAIAMLLAKIDGKPFTTEIPMEVWDQV
                     EPAAALADTAAANLAVICTGGVVPWGNPDGFKTYRNTFWRKYNFSEVKSLEAGKWEAV
                     HGGYNVAFMNQNPHYGMPLDALRSLEADGRIGRGKLYPAYYVIPGNQGSPAVMRRVGQ
                     EIAADLKKDGVEAVLLVATUGTCSRSGAVISKELDRAKLPVAMISAMYPVAEQVGASR
                     IVKGVSIPHPCGDPSLPAELDAQLRREIVSTALKALEAKVESSTVFTPKIASSA"
     gene            4304..5608
                     /locus_tag="VE170_01575"
     CDS             4304..5608
                     /locus_tag="VE170_01575"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYJ14146.1"
                     /translation="MRLELAEFPVAGIELADRFHYAVGQLTVHQSAIEQAVLQESRIE
                     AAWLDVVSPGDKVRITGIRDVVEPRVKVSGSGQVFPGVLGPVEPVGGGRTHCLSGMAV
                     FVTAEYDGMVRAGLGVQRSAILDMWGDGAAASPFSRLNGLVLNLRLAKGLSELTAHTI
                     IQRAALETAKRLAEVTIGLTAEQIETFELGGADPNLPKVLLIQGCLTDAGNPHSGVGY
                     FGLPIRDSLATTVHPNEFIDGAFTMYATRCLAYFPVTWDWQNHPLLLGLYREHRDKVN
                     FLGVILERIAFDTFHGKEVIAQNTAQLATMLGADAALVSWIGSGNAFVDVMLTLRACE
                     ERGIKTNLITYEYGGKDGVDSPLLFYTPAASAMTSTGSRDRWIELPAAEKIVGPYEEI
                     KVLTYPGAPLTSARGPFTLDARDMILGGVDYWGRGGWNCAAY"
     gene            5625..6638
                     /locus_tag="VE170_01580"
     CDS             5625..6638
                     /locus_tag="VE170_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14147.1"
                     /translation="MKRLATVIAVQLIALLATMEFAGAAAAPSKIVIAYASLNERGAG
                     ALLVARDQGFFRKYDLDVNLIYMRNAAVALSALSAGEAQFYSGSANGSVLGAIANGLD
                     LAFIAGLANKLNGTMVASAAIASPADLRGKNIGIQSIGGGIWMITQLVLEHWGLDPKR
                     DGINLRIVGDETVMVQSLMLGTVDATYAGYAYATMLKKQGFRVMADLTKLGVPFQSTA
                     ILARRSYLNSNSATAEKVIRALVEAIAFIQRPANKAAVVATLTKNLQIVRPEEAEEGY
                     EVIRNVYERRAFPNPEGINNAIRLLGLGNEKIRQLKAANIVDDSIVRKLDKEGLLSEI
                     VPR"
     gene            6705..>7896
                     /locus_tag="VE170_01585"
     CDS             6705..>7896
                     /locus_tag="VE170_01585"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYJ14148.1"
                     /translation="MQLILAEFPVRQIRLGDVTRYHDELLEVDREGLTRLVRQDPRIE
                     TAQIDAVAPGEPVRVTGIRDVIEPRIKFDNDAQVFPGVIGPVVTVGDGVTHRLSGMAV
                     LASAAYEGTIRAGTGVQRSAILDMWGAGAAASRFSGLRHLVLSLEIRAGLGELEAHQA
                     IQQAAYQVACKLAETTEGLAAARTEIFPSPINRGGRPRVVLIQGCITQAQQPHAGVSY
                     CGLPIRESLPTALNPNELFDGAMTMNTTRGIGYYPTTWDWQNHPLALALYREQAADNI
                     DFAGVIIERISYETHHAKEVVAHNTARLAKSLGADAVLLTWLGSGNAFVEMMLTIRAC
                     ERLGIKTVLVTYEYGGKDGVDSPLLFYAAEADAAVSTGSRDRWIELPAAEKVIGAYQD
                     IRVLT"
BASE COUNT         1497 a         2275 c         2554 g         1570 t
ORIGIN      
        1 cctgagcggg gaggcttgcg gttttccgtt tgagcagtcg aaagagtatc tcgtctacgt
       61 ctttgccgag ccccgagatc tccaaaccgg gatctgcacc gggacgaaga gcatcgccga
      121 ggcggcgcaa gagatggagc aactagacga gatcgtcgcc gctgccaaga aatcaggagc
      181 gacgaatgtt taccgataaa cagcggggtg tcatccgcgg cgtggttcct gccgcgacag
      241 ccacggtcgt cggtttcggc ggtgggtcgt tgttaattcc catgagcgcg cttgcggcgg
      301 acgaaccggc ggcgcgaatc gcctggactt tgcagtgggc tgtgttgcca attttctctc
      361 tgatgatttt catcatgcgg gttgccaacc atcgattttc cacgccggaa gatatcgacg
      421 gcagcgggtt gaccagcggc acctcacgca tattggtgct gcgggcaatt ttgcagaata
      481 ccctggagca ggccgtgctg gcggtcgccg tttatatgat ttgggccgcg gtcatgccgc
      541 atagctggct gcgggcgatt ccgatggccg cgctattgtt tgtcgtgggc cgcatcctat
      601 tcgccagcgg ttacgaacgc ggcgccgccg gacgggcgat gggattcggc ctgacggcgt
      661 acccgacctt cggcatgttg acgacagtgg cgacgttttt ggcgtggcgg gtggtgcgtt
      721 agaacgccgc ctatcaacgt cattggttcg aaagcaatta tggcaaccgc cggcttccat
      781 ggtgtttttc cctatctgat atccccgatc gattccgccg gtgcggtcaa gagcgatgtt
      841 ctggcgcgcc tgtgcgacga ccttatcgat gccggcgtcc atggcctgac gccgctcggt
      901 tcgaccggtg agtttgctta tctaaactgg gcgcagcggc gccgggtggt cgaagtcgtc
      961 gttggggcgg cgcgggggcg agtgccggtg gtggcgggag tggcatcgac gacgatcgcg
     1021 gatgcgacgg cgcaggcgcg ggagttcgcg cagatgggcg cgagcggcat tctcgccatt
     1081 ctcgaagcct atttcccgct ctcggacgcc ggcgtgttcg attacttcaa ggcgattgcc
     1141 gatgcggttt cgctccccgt ggtgctctat accaatccca cgtttcaacg ctcagatttg
     1201 agcctgccgg tgattgaccg gctcagtcac gttgccaaca tcggttatat caaagacgct
     1261 tcgaacaata ccggccggct gctgtcgatt ctaaatcaag tccaggggcg gatgaaagta
     1321 tttgccgcgt cggcgcatat tcccgccgcg gtcatgttga tcggcggtgt cggctggatg
     1381 gcggggccgg cctgtgtggc gccgcgccaa agtgtggcgc tttacgaaac ctgccggtgc
     1441 ggcaattgga cgggagcgat ggagttgcag cggccgctgt gggcgctcaa ccaggccttc
     1501 gccaaataca atctcgccgc ctgcatcaaa ggcgccctcg aacttcaggg ttacgcggtg
     1561 ggtgcgccat tgccgccgca agcgccgctg acggcggagg cgcttgcaga cgtgaagcag
     1621 acgctaattg ctctcggcgc gctgcaacat tgattgcaat gttacaccgg ctggagttat
     1681 gcaaagaagc gcgctacatg gtcatgaggg gtcgatgaat cggccggaaa ggagccagcg
     1741 atgatgtaca ggaaccgggt tcatttcgcc gccggcgcga tggcggcgat atggctggtt
     1801 ggttgccttg gcattgcgct ggcggcgacg ccggcgccgc acaagcgcgt gcgcatactc
     1861 tttgccgact tcagcgagcg catgggcttg ttctttgtcg ccaaggatca acgtttcttc
     1921 gaagagcagg gcttggacgc agacctcgtg caggtcaaca ccgggccggt ggcggtggcg
     1981 gcgatggccg ccaacgaagc cgaattttac accgtgtcgg ccaccggttc cgctctcggc
     2041 gcgatctcaa gcggcctcga tctggtctgg gtggcgggaa tgatcaataa actcgatggt
     2101 tatttttacg tctcgccaaa agtccaaaag cccgaggatc tcaaaggcaa gacgctcggc
     2161 gtgcaaagca tcggcggcgg catctggatg ttcgtcatga tgacgttgga tcattggggc
     2221 ttgagcccgg agcgcgataa gatccagatg cgggtaatcg gcgaccagtc ggtgctggtg
     2281 caatcgttaa atgccgggtt gatcgatggc tcggtgtggg gctacgctta caatgctgcc
     2341 ctgcaacgca gcggcggccg gctgttggcg gatttgacgg cgctcaacat tccctatcag
     2401 ggaaccgggc tggtggcgcg gcgggcgttt atcgccagct cgcccgacgt ggtagaaaag
     2461 actttgcgcg gttttgtcaa agccaacaat ttcgtccgcg acaaaagcaa tcaagcggcg
     2521 gtggtgcgca gcattcgcaa atggctgcgg ctttcgccgg atggtaacgc cgatgatctg
     2581 tacgaacgca tgcggctgct ctacgaccgg cgcatcgcgc caacccgcga gggcatgcaa
     2641 aacgccttgc gggtgctcgg caaggtcgag cccaagtaca gcaagctaaa agtggaagat
     2701 ctggttgacg accgcatcgc ccgcaagttg gagacgccat gagtttgccg cggttgcgtt
     2761 gagtcagctg tcgatgaaaa gagatacctc atgatccgaa tcgtccatat cctaaatcag
     2821 ttttttgccg gcatcggcgg tgaggacaag gccgatatcc cggttgccgt cgccgacggc
     2881 gcggcgggtg cggcgcgcgg tctgcagagc cagctcgccg agcgcggcca gatcgtcgcg
     2941 acggtttact ttggcgacaa ttattttcat gaacacaaag acgacgcgct ggcggcgatt
     3001 accgccgaat tagaaaagca aaaacccgac gtggtggtcg ccggtccggc gtttaacgcc
     3061 gggcgttatg gcctcgcctg cgtggagatt tgccaggcgg tgagcgaaaa gcttggtctg
     3121 cgctgcgtca ccggcatgga gccggaaaat cccggcgtcg gtgtctaccg cgacaatcac
     3181 aacgacaagg tatttttgct gccgacggcg gaaaccgcct cgggcatggc gcgcgcgctg
     3241 gcggcgctgg ccaccttcgc ctgccgtttg gccaatggtg aagagatcgg accggcggcg
     3301 gacgagggct atttgccgcg cggcattcgc cggctggtgc gggtcgacaa gagcggcgcg
     3361 gatcgcgcca tcgccatgtt gctcgccaag atcgacggca agccgttcac caccgagatc
     3421 ccgatggaag tctgggatca ggtggagccg gcggcagcgc tggccgacac ggcggcggcc
     3481 aacctggcgg tgatttgcac cggcggcgtc gtgccctggg gcaatcccga cggcttcaag
     3541 acctaccgca atactttctg gcgtaaatat aacttctccg aggtgaaatc gttggaggcc
     3601 ggcaagtggg aagcggtgca tggcggttac aacgtcgctt tcatgaatca aaatccccac
     3661 tacggcatgc ccctcgacgc gctgcggtct ctggaagccg acggccgcat cggccgcggc
     3721 aagctctatc cggcttatta cgtgatcccc ggcaaccagg gctcgccggc ggtgatgcgc
     3781 cgggtcggcc aggagattgc cgccgattta aaaaaagacg gggtcgaagc ggtattgctg
     3841 gtggccacgt gaggcacctg tagtcgcagc ggcgctgtga ttagcaaaga actggaccga
     3901 gcgaaactcc cggtggcgat gatcagtgcg atgtatccgg tggcggaaca ggtgggcgcc
     3961 agccggatcg tcaaaggcgt gtcgatcccg cacccgtgcg gcgacccgag cttgcccgcc
     4021 gagctggacg cgcaattgcg ccgcgagatt gtctcgacgg cgctgaaggc gctcgaggcg
     4081 aaggtcgaat cgtcgacagt gttcacgccg aagatcgcaa gcagcgcgtg aggcaagtcg
     4141 tgatcgtgac cgcggttcgg ggttcgttca tcccgtaatg attatgagtt catcccccgt
     4201 taaaaagaga aggggagatt ttcgcatgct cgcaaacttc aaatccctct cgatccccct
     4261 ttttcaaagg gggatgctat gagtattcgt cctggaaatt tccatgcgtc ttgagttggc
     4321 ggagtttccg gtggcaggca tcgagctcgc cgaccggttt cactacgccg tcgggcagtt
     4381 gaccgttcac cagtcggcca tcgagcaagc ggttttgcag gagagccgta tcgaggcggc
     4441 ctggctcgat gtcgtctcac ccggcgataa agtacgcatc accggcatcc gcgacgtcgt
     4501 cgagccgcgg gtcaaagtga gcggctcggg gcaagtgttt cccggcgtgc tcgggccggt
     4561 ggaaccggtc ggcggaggcc ggacccattg cctctccggc atggccgtgt tcgtcaccgc
     4621 cgagtacgac ggcatggtgc gcgccgggct cggcgtgcag cgcagcgcga tcctcgacat
     4681 gtggggcgac ggcgcggcgg cttcgccctt cagcaggctt aacggtttgg ttctcaatct
     4741 gcgtttggcg aaggggttgt cggaactcac cgcgcacaca atcattcagc gtgccgcttt
     4801 ggaaaccgcc aagcgcttgg cagaggtgac gattggcttg actgcggagc aaatcgaaac
     4861 ttttgaattg ggcggcgccg atccgaacct gcccaaggtc ttgctcattc aaggctgttt
     4921 gaccgacgcc ggcaatccgc attccggcgt cggctacttc ggactgccga tccgcgattc
     4981 gctggcaacg acagtgcacc ccaacgagtt tatcgacggc gcgttcacca tgtacgcgac
     5041 ccgttgcctg gcctatttcc cggtcacctg ggattggcag aatcatccgc tgctgctcgg
     5101 cctctaccgc gagcaccgcg acaaggttaa ttttcttggt gtcattctcg aacgcatcgc
     5161 cttcgatacc tttcacggca aggaagtgat cgcccagaat accgcgcagc tggcgaccat
     5221 gctgggcgcc gacgcagcgc tggtgtcatg gatcggctcg ggcaacgcct ttgtcgatgt
     5281 catgctgacc ttgcgcgcct gcgaggagcg cggcatcaag accaatttga tcacctacga
     5341 atacggcggc aaagacggcg tcgattcgcc gctgctattt tacacgccgg cggcgagcgc
     5401 catgaccagc accggcagcc gcgatcgttg gatcgaacta ccggcggcgg aaaaaattgt
     5461 cggtccctac gaggaaatca aagtgttgac atatccgggc gcgccgctga cttcggcgcg
     5521 cggcccgttc accctcgacg cccgcgacat gattctcggc ggcgtagatt attgggggcg
     5581 cggcggctgg aactgcgccg cttattgatg ccaacggagc gaaaatgaag agactggcaa
     5641 cggttattgc ggtgcaatta attgctttac tcgcgacgat ggagttcgcc ggcgccgccg
     5701 cggcgccgag taaaatcgtc atcgcctatg cctcgctcaa cgagcgcggc gccggtgctt
     5761 tgctggtggc gcgggatcag ggcttctttc gcaagtacga tctcgatgtc aatctgatct
     5821 acatgcgcaa tgccgcggtg gcgttgtcgg cgctatcggc gggggaggcg cagttctatt
     5881 cgggttcggc caatggttcg gtgctcggcg ccatcgccaa cggcctcgac ctggcattca
     5941 tcgccggctt ggccaacaaa ctcaacggca ccatggtggc gtcggcggcg atcgcgtcgc
     6001 cggccgattt gcgcggcaaa aacatcggca tccagagcat cggcggcggc atctggatga
     6061 tcacccagct ggtgctcgaa cattggggac tcgatccgaa acgggacggc atcaacctgc
     6121 gcatcgtcgg cgacgaaacc gtcatggtgc agtcgttgat gctcggcaca gtggatgcga
     6181 cctacgccgg ctatgcctac gcgaccatgc tcaagaagca gggctttcgg gtgatggccg
     6241 acctgaccaa actcggcgtg ccgtttcaga gcacggcgat tttggcgcgc cgaagttatt
     6301 tgaactcgaa ctccgccacc gcggagaaag tgatccgtgc tctggtcgaa gcgatcgctt
     6361 tcattcaacg gccggccaat aaggctgcag tggtggcgac cctgacaaaa aatttgcaga
     6421 tcgtccggcc agaggaagcg gaagagggct acgaagtgat tcgcaacgtc tacgagcggc
     6481 gcgcgttccc gaatcccgag gggatcaata acgcgattcg tttgctcggc ctcggcaatg
     6541 aaaaaattcg ccagctcaag gccgctaata tcgtcgacga ttcgatcgtg cggaaattgg
     6601 ataaggaagg gttgctaagc gagatcgttc cacggtgaat aattggtgcc gtctgccgta
     6661 ggggcgaaaa atctttcgcc cctaccatcg tcaacccatt gccaatgcag cttatattag
     6721 cagaatttcc ggtgcggcag atccgcctcg gtgacgtaac tcgatatcat gatgaactgt
     6781 tggaagttga ccgggagggg ctgactcggc tcgtgcgcca agacccgcgc atcgagacgg
     6841 cgcaaataga cgcggtagcg ccgggcgaac cggtgcgggt caccggcatt cgcgacgtca
     6901 tcgagccgcg gatcaagttc gacaatgacg cgcaagtatt tcccggggtg atcggtccgg
     6961 tggtgacggt gggcgatggc gtgacccatc ggctttccgg catggccgtg ctcgccagcg
     7021 cggcgtacga gggcacgatt cgcgccggca ccggcgtgca gcgcagcgcg atcctcgaca
     7081 tgtggggcgc gggcgcggcg gcttcgcgct ttagcggcct gcgccatttg gtgttgagcc
     7141 tggaaattcg cgccggcttg ggcgaactgg aagcgcacca ggcgatccag caggcggctt
     7201 atcaagtcgc atgcaagctc gccgaaacca ccgaaggact ggccgcggcg cgcacggaga
     7261 ttttcccttc gccgataaat cgcggcgggc ggccgcgggt agtattgatt caaggctgca
     7321 tcacccaggc gcagcagccc cacgccggcg tgagttactg tggcctgccg atccgcgagt
     7381 cgttgccgac ggcgctgaat cccaatgaat tgttcgacgg cgcgatgacc atgaatacga
     7441 cccgcggcat cggttattat ccgaccacct gggattggca aaaccatccg ttggcgctgg
     7501 ctttgtaccg cgagcaagcg gcggacaata tcgattttgc cggcgtcatc attgagcgca
     7561 tttcctacga gacccatcac gccaaggaag tcgtcgccca taacacggct cgactggcaa
     7621 aaagtttggg cgccgacgcc gtgctgctga cctggctcgg ctcgggcaat gccttcgtcg
     7681 agatgatgtt gacgatccgc gcctgcgagc ggctcggcat caaaaccgtt ctcgtcacct
     7741 acgagtacgg cggcaaagac ggtgtcgact cgccgctgct attctatgcc gccgaagcgg
     7801 acgccgcggt cagcacaggc agccgcgacc gctggatcga attaccggcg gcggagaaag
     7861 tcatcggcgc ctatcaggat attcgcgtct tgacct
//
LOCUS       DASQKS010000061         6499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1294636, whole genome shotgun sequence.
ACCESSION   DASQKS010000061 DASQKS010000000
VERSION     DASQKS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294636"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VE170_01590"
     CDS             complement(<1..870)
                     /locus_tag="VE170_01590"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14149.1"
                     /translation="MTQWRFVGSVLSILSFYALVLSSDAAFAADAPAKTKIRIATAAP
                     SLSYFPIYAAVQKGFFARRGFDVEMIQMAPSLTAAALLNRSVDYTIIPSTIATAAARG
                     APAKVIMFASVKLQHSLVVRPDINAVTELAGKKIAGSGFGNLVAYEIQYLIERYKLGA
                     NTTIINAPSSIDRLIAVQRGLAEAAIIAAPADIKGEEMGLKRLVQMGTLLQIPQAGLA
                     ATDEKIKANRAEVIEVLKAAIEGLEYTANQREDATVLIGKWMALTPIQASKAYDSVRD
                     TYSRDGVPTAEQSK"
     gene            complement(867..1901)
                     /locus_tag="VE170_01595"
     CDS             complement(867..1901)
                     /locus_tag="VE170_01595"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14150.1"
                     /translation="MMILASTPLLKHSKFVFVFVLVLQTLMVNGGELRAQALIKVPFP
                     YSPINSSSLPWMIAKDAKLFEKHGLDVDLVYFGASSLILQTMLSGSANYGGFAGPAII
                     SNVLKGGDVITIGALTPMSISLLTRASIEKGEELRGKKIGISRLGAVPHLAIQLILDK
                     FAIKDATILQMGGQPEAAAALRRGIVDGAMVSAPLSFQLVKEGFRELIGTPEYQRLGI
                     NFTTQGIAARRSFAARNRDVTVRLIKGTMEGVKTMWANESLAKKVLAKYTRQSDAELL
                     DRTYKEALATVARDPTSSKDSVVSTTRLMADLGLADRAAVNSTPVEAFYDNSYIDEIK
                     QSGFLKELWR"
     gene            complement(1904..2899)
                     /locus_tag="VE170_01600"
     CDS             complement(1904..2899)
                     /locus_tag="VE170_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14151.1"
                     /translation="MKLSLIISLLLLLTNLAFAQDAKLIEAAKKDGKLIVYGTMQSDI
                     FELLQKSFQKKTGIAIDYWRTSATKVMERALSEARAGKALFDLVMSTEDTQRIMLKEG
                     ILTKYESPMVKDFPKDSIDPQLGPRARNHIIGVMYNKNVITPADAPKTLEDLVKPQYR
                     GKLVMADPTLHTTTAQWLANLHKLMGKDRADKFIRDLAAMKPILVESFAPSADRVTTG
                     ETPIGITYVYYVFLNGQKGAPVDYVRGDYRLLGDASYMSIFHKAPHPNAARAFVDFFL
                     DDESMNIMAKAGEFVNRKGVYPPVPEADKIKFVEMDDLGESFYAEKRKEFQKLFR"
     gene            complement(3036..4085)
                     /locus_tag="VE170_01605"
     CDS             complement(3036..4085)
                     /locus_tag="VE170_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14152.1"
                     /translation="MIESKVAKVIDADGHIYENHEEIEEYFEGHYRGMRRARAYPLFP
                     SLDGWPRGLGAGRPDKVTETKPEDVIKFVDNMGLESTVLYPTAALAIGLVQEPAWACA
                     VSRAYNNWFHHRYIKRDSRLKGVAVLPIQDPQKAAEELRYAVEKLDMVGAFLPSALVV
                     SKAFGHADFAPIFRTAEQLGVPLGVHGAPSRGFGFDFFEDQGQVHALEHPFPLMIQFT
                     SIICDGVLEKFPKLKIAFLEAGCGWLPYMMDRLDYEYETRGARSFPNMKKKPSDYVKD
                     CPVYVSCEPEEHSLAYVSQVIGADRIMFPSDYPHERVYGEFLKDIPELLKRPELSDDA
                     KQKILYDNAKEFYRI"
     gene            complement(4364..4972)
                     /locus_tag="VE170_01610"
     CDS             complement(4364..4972)
                     /locus_tag="VE170_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14153.1"
                     /translation="MISEDWRATPLTASGLDIDLYRHRESKRKVVIARNVYGDDAEIV
                     LRTLYGKGIRQAIYLGAAGAVNDYRIGDVVIPNEFVDRNMVSTPFPHNSAQAYLKELP
                     GDVTVHKPFKHGWAQTLFEETKPLLLKWKALGVGAVDIEGLYLGKFAQRHKDLKIAAL
                     FIISDETLGENTIEDSNANRGAIDATVTKLVAHLYPKIIESR"
     gene            complement(5046..>6499)
                     /locus_tag="VE170_01615"
     CDS             complement(5046..>6499)
                     /locus_tag="VE170_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14154.1"
                     /translation="SFVFVLFSFGQNSQPLLTMLARVLFLIGVTLLDYRAAAGESVAL
                     YSASFDPPTRSQLRMLRCVLGDAELPQECREPAAQISRLIIWVDAAPNVEAHASAKER
                     VLMLNRSLQAHRPRLEISAHAPSAIEEKRRALLADETIDKIFLLIDGDTNRRPSSTQP
                     RSPKLVQLLFPLVHEARLSADLAAFDRKVDPQAAAIIDQLGLYREVSGDLAELQRELF
                     DEGWRAYLSDLVIACPLAIDRNTCTSLAASWRGIPTVANPPRNISAGESSLAQLAYAG
                     TQSESRWAEKFTETSMTAVREPADRDRLKAVAEDLAERTLRDLPRGKLPHLHRLVTPR
                     RAAKTKGLRVSRSPVTCAAPNESYSADMDRYLADRFPQAFSRFLKHDYRRYSDQAVEL
                     YLHDHPLEQAYDLHRRDGFSDFYFVQTRRGQLHRDISSRGESTAARLPRCSHQCARQG
                     PARKCPLPAQPHGYLRHAPLGSSITAATSVRAQ"
BASE COUNT         1300 a         1733 c         1970 g         1496 t
ORIGIN      
        1 tttgctttgc tccgctgtcg gcacgccgtc gcgggagtag gtgtctctca cgctatcgta
       61 cgctttactc gcttggatgg gtgtgagcgc catccatttg ccgatcagca cggtggcgtc
      121 ttcccgttga tttgccgtgt attccaagcc ttcgatggcc gctttcagaa cttcgatgac
      181 ttcagcgcgg ttggctttaa ttttttcgtc ggtggcggcc aggcccgcct gagggatttg
      241 tagcagcgtg cccatctgca ccaagcgctt gaggcccatc tcttcgcctt tgatatcggc
      301 aggggcggcg atgatcgcgg cctcggctag gcctctttgc accgcgatca agcggtcgat
      361 cgaggacggc gcattgatga tggtggtatt ggcgccaagc ttgtagcgct cgatgagata
      421 ctggatttcg taggcgacca gattgccgaa gccgctgccg gcgatttttt tgccggccaa
      481 ttccgtgacc gcgttgatgt ccgggcggac gaccagggag tgttgcagtt tcaccgaggc
      541 aaacatgatg actttcgccg gcgcgccgcg ggcggcggcg gtggcgatgg tgctggggat
      601 gatcgtgtaa tccaccgagc ggttgagcag cgcggccgcg gttaaactag gggccatctg
      661 gatcatctcg acgtcgaagc cgcgccgggc gaagaagcct ttctgcaccg cggcgtagat
      721 cggaaagtag ctgagcgacg gcgccgcggt ggcgattctg atcttggttt tcgccggggc
      781 gtcggcggcg aaagcggcat cgctcgatag taccagcgcg tagaaagata gaatggatag
      841 gacagatccg acaaagcgcc attgggtcat cgccacagct ccttaagaaa gcccgattgt
      901 ttgatctcat cgatgtagct gttgtcgtag aacgcttcta ctggcgtgct gttgaccgca
      961 gcgcggtcgg cgaggccgag gtcggccatc agccgcgttg tcgaaacaac ggaatctttc
     1021 gatgaggtcg gatcgcgggc aacggttgcg agcgcctctt tgtaggtacg gtcgagcaat
     1081 tcggcgtcgc tttggcgcgt gtatttggcg agtacttttt tggccaggct ctcgttcgcc
     1141 cacatggttt tgacgccttc catggtgcct ttgatcaggc gcaccgtcac gtcgcggttc
     1201 ctcgcggcaa aggaccggcg cgcggcgatg ccctgggtgg taaaattgat tcctaacctc
     1261 tggtactccg gtgtgccgat caactcgcga aatccctctt tgaccagttg aaacgatagc
     1321 ggcgccgaaa ccatagcacc gtcgacgatg ccccttcgga gcgccgctgc cgcttcgggc
     1381 tggccgccca tttgtaaaat ggtcgcgtct ttgatcgcga atttatccag gatcaactga
     1441 atcgctaagt gcggcacagc gccgaggcgg ctgatgccga tcttcttgcc gcgcagctct
     1501 tcgccttttt cgatggatgc ccgggtgagc agcgaaatgc tcatcggcgt gagagcgccg
     1561 atggtaatca cgtcgccgcc tttgagtacg ttggagatga tcgccggtcc ggcaaagccg
     1621 ccatagttgg ccgaacctga cagcatcgtc tggagaatca gcgacgaagc gccgaagtag
     1681 accaagtcga catcgagccc atgtttctca aacagcttgg catccttggc gatcatccaa
     1741 ggtaaactgc ttgagttgat aggcgagtag gggaagggaa ccttgatcag ggcttgcgcg
     1801 cgtaattcac caccattcac catcaaagtc tggagcacaa gcacgaagac aaacacgaat
     1861 ttagagtgtt tgagtaatgg ggtgctggct agaatcatca taattagcga aacagcttct
     1921 gaaactcttt gcgtttctcg gcgtagaaag actcgccgag atcgtccatc tcgacgaact
     1981 tgatcttatc tgcttcgggc accggcgggt agaccccttt acggtttacg aattcgccgg
     2041 ccttggccat gatgttcatg ctctcgtcgt cgaggaaaaa atcgacgaag gctctggcgg
     2101 cgttgggatg gggcgccttg tgaaagatcg acatgtagct ggcgtcgccc aagagccggt
     2161 agtcgccgcg gacgtagtcg actggcgcgc ctttttggcc gttcaaaaat acataataga
     2221 cgtaagtgat gccgatgggc gtctcaccgg tggtgacgcg gtcggcggac ggggcgaagg
     2281 attccactag gatcggcttc atcgcggcga gatcgcggat aaatttgtcg gcgcggtctt
     2341 tgcccatgag tttatgcaag ttggctagcc attgcgccgt cgtcgtgtgc aaggtcggat
     2401 cggccatgac cagcttgccg cggtattgcg gcttgaccag gtcctcgaga gtcttgggcg
     2461 catcggcggg cgtgatgaca ttcttgttgt acatgacgcc gataatatga ttgcgcgctc
     2521 gcggacccaa ttgcggatcg atagaatctt tggggaaatc cttcaccatc ggcgattcgt
     2581 atttcgtcag gatgccttct ttgagcatga tccgctgggt gtcttcggtg gacatgacca
     2641 gatcgaacag cgccttgccg gcgcgcgctt cgctgagcgc gcgctccatt accttggtcg
     2701 ccgaagtgcg ccagtagtcg atggcgatgc cggttttctt ttgaaacgac ttttgcagca
     2761 gctcgaaaat atcggattgc atggtgccat agacgatcag cttgccatct ttttttgccg
     2821 cttcgatgag cttggcgtcc tgggcgaagg caaggttcgt taaaagcagc agtagcgaga
     2881 tgattaaaga tagcttcata gtgatatgaa cccctgagct ggaaaggcca gagccaagag
     2941 catcgagcaa aaggctcgcc gagagattgt tttgtgcatg agtttcaccc tcaccctcgt
     3001 cctttcccac ttcgtgggcg aggaaatatg agggatcaga tgcggtaaaa ctctttggcg
     3061 ttgtcgtata gtattttttg tttagcgtcg tcggagagtt ccgggcgctt gagcagttct
     3121 gggatgtcct tcaagaattc gccgtagact cgttcatggg gatagtctga gggaaacatg
     3181 atgcggtcgg cgccgatcac ttggctgaca taggcgaggg agtgttcttc cggttcacag
     3241 ctgacgtaca cgggacagtc tttgacgtag tcgctcggct tctttttcat gttggggaac
     3301 gagcgggcgc cgcgggtttc gtactcgtaa tccaaacgat ccatcatata cggcagccag
     3361 ccgcagccgg cttcgaggaa ggcgattttg agtttgggaa acttctccaa gacgccgtcg
     3421 caaatgatgc tggtgaattg aatcatcagc gggaacggat gttcgagggc gtggacttgg
     3481 ccctggtctt cgaaaaagtc gaaaccgaag ccgcggctgg gcgcgccatg gacacccaaa
     3541 ggcacgccca actgctcggc cgtgcgaaag atcggcgcaa agtcggcatg gccgaaggct
     3601 ttgctgacga ccaatgccga gggcaggaaa gcgccgacca tgtcgagctt ctcgactgcg
     3661 taacgcagct cttctgccgc tttttgcgga tcttgaatcg gcagcacggc gacccctttc
     3721 aggcgcgagt ctcttttgat gtagcggtgg tgaaaccaat tgttataggc ccgcgagacc
     3781 gcgcaggccc aggcgggctc ttgcaccaac ccgatggcga gggcggcggt gggatagagc
     3841 accgtgcttt ccaaccccat gttgtcgacg aacttgatga catcttccgg tttggtctcc
     3901 gtgactttgt cagggcgccc ggcgcccagg ccgcgcggcc agccgtcgag ggatgggaaa
     3961 agcggatagg cgcgcgcccg gcgcatgccg cggtagtggc cctcgaagta ttcttctatt
     4021 tcttcgtgat tctcgtaaat atggccgtcg gcgtcgatta ctttggcgac tttagattca
     4081 atcatggttc tgcctttcta ttttttgccc atccggaata gtaagccgcg gcgctgccaa
     4141 ctatagagaa cgatctccac cgggtgtatc gcggcatgaa ataagaaatt acaaatttcc
     4201 tatgttagcc gtacggtgag attcagtact ccagctggtt tgccgatctc tcactgcatc
     4261 agccagctct atccgttttt tcgaaatatg gctaggtgta tcgaactaac cctcggaagc
     4321 gttgaagcgt attctatttg gttgttgcat catcggtcgc gggtcagcgt gattcaataa
     4381 ttttcgggta gagatgcgcc accagtttgg tcaccgtggc gtcgatcgcg ccgcgattgg
     4441 cgttgctgtc ttcaatggtg ttttcaccga gagtctcgtc ggagataata aacagcgctg
     4501 cgatcttaag atccttgtgc cgttgagcga atttgccaag gtataagcct tcgatgtcga
     4561 cagcgcccac gccgagggct ttccacttca acaagagcgg cttggtctcc tcgaacagag
     4621 tttgtgccca accgtgtttg aatggtttat gcacggttac gtcgccgggc aattctttca
     4681 ggtacgcttg cgccgagttg tgcgggaatg gagtcgaaac catattgcga tcgacaaatt
     4741 cgttggggat gaccacgtcg ccgatgcgat agtcgtttac cgcccccgca gcgccaaggt
     4801 aaatcgcctg gcgtatgcct ttgccgtaaa gcgtcctgag aacaatttcg gcgtcgtcgc
     4861 cataaacgtt gcgcgcgatg acgacctttc gcttgctttc gcggtgacga taaagatcga
     4921 tgtctagccc ggacgcggtc agcggcgtcg cgcgccagtc ctcgctgatc aatttgcgcc
     4981 gccattcacc tctgaagccg aataatagcc agtcgcccgc gttgagtttg agcgattttc
     5041 cctcactatt gagctcgaac agaggttgcg gccgtgatgc ttgaacccaa cggtgcgtgg
     5101 cgaagatacc cgtgcggttg agctggcaga ggacatttgc gcgccggtcc ctgccgcgca
     5161 cactggtgag aacaacgcgg taagcgcgcg gctgtgcttt caccgcgaga tgatatgtcg
     5221 cgatgcagtt ggccgcggcg ggtctggaca aaataaaaat cgctgaagcc atcgcggcgg
     5281 tgcagatcgt aagcttgctc aaggggatga tcgtgcaggt aaagctcgac cgcttggtcc
     5341 gagtacctac ggtaatcatg tttgaggaaa cgcgagaaag cctgggggaa tcgatcggct
     5401 aagtaccggt ccatgtcagc gctgtaagac tcgttcggcg cggcgcacgt gaccggggag
     5461 cggctgacgc gcaacccctt ggttttcgca gcacgtctcg gcgtaaccag cctgtgcaaa
     5521 tggggcagct tgcctcgcgg taggtcgcgt agggtccgtt cggcgaggtc ttcggcgacg
     5581 gcttttaacc ggtcacgatc cgccggctcg cgcactgcgg tcatcgaggt ttcagtgaat
     5641 ttctccgccc aacgactttc agactgagtg cccgcgtaag ccagttgggc cagggacgat
     5701 tcgccggctg atatgtttcg cggcggattg gccaccgtcg gaatccctct ccacgaggct
     5761 gctaacgacg tgcaagtgtt acgatcgatc gccagcgggc aggcgattac cagatcgctt
     5821 aggtaagcgc gccagccctc gtcgaaaagc tcccgttgga gttctgccag atctccgctc
     5881 acttcccggt aaagtcccag ttggtcgata atcgccgcgg cttgcggatc gaccttgcga
     5941 tcgaacgcgg ccaagtctgc gctcagtcgc gcctcgtgca caagtggaaa tagtaattga
     6001 accagcttcg gtgatcgagg ctgcgtcgac gacggccgcc gattggtatc gccatcgatt
     6061 agcaaaaaga tcttatcgat ggtttcgtcg gcaagcagcg ctcgtcgttt ctcttcgatc
     6121 gccgacggcg cgtgggcgga aatctccaag cgcgggcggt gtgcctgcaa ggagcggttg
     6181 agcatcagca cccgctcctt ggctgaagca tgcgcttcga cattcggcgc ggcatcgacc
     6241 caaatgatca ggcgggaaat ttgggcggca ggttctcgac actcctgtgg caattcggcg
     6301 tctccgagga cacaacgcag catgcgtagt tgcgatcggg tcggcggatc gaagctggcc
     6361 gaatacaaag cgacggattc gcccgcggcg gcgcggtaat ccagcaatgt gacgccgatc
     6421 aggaataaaa cccgcgcaag catcgttaaa agaggctgac tgttctgacc gaacgagaat
     6481 aggacaaaaa caaacgaga
//
LOCUS       DASQKS010000062         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4655084, whole genome shotgun sequence.
ACCESSION   DASQKS010000062 DASQKS010000000
VERSION     DASQKS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4655084"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VE170_01620"
     CDS             <1..284
                     /locus_tag="VE170_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYJ14155.1"
                     /translation="SGTSVGANYRAACRVKSTADVIAKLRIVEEEADESAYWMELLIE
                     SGLLPASRLSELLQESNEIVAMTVASIRTLQKRTGSSPINRQSKIENLK"
     gene            293..1267
                     /locus_tag="VE170_01625"
     CDS             293..1267
                     /locus_tag="VE170_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14156.1"
                     /translation="MKQIIGLLVVIVILTACRATVGAQPAKPERIGVIHLGEVFATVV
                     DGLRAGLKELGMQDGKQFVLDVHDLKGDLKSAQPVAELLERDKVKLIFTNTTPVTASA
                     FKATKNISIVFAVGSDPVASGYAKSFGQPGGRLTGIQYLARDLTGKRLEILKEILPKL
                     RQVVAFYDPGNTIAVEGLKLGREEAQRVNVKLAERQVKSIEELNTGLDALKAGEFDAY
                     LYMPDPMVVSQAPRIVEVARAKRLPTMFHDQVVVARGALASYGQSYFEIGRRSAKYVQ
                     RVLNGTPPAELRIDTVDDIELAFNLVTAKQLGVTIPPNVLARAQKVIR"
     gene            complement(1404..2285)
                     /locus_tag="VE170_01630"
     CDS             complement(1404..2285)
                     /locus_tag="VE170_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HYJ14157.1"
                     /translation="MTARQTLKQLLKRNKLLVAPGCFDGLSARLVQEAGFEAAYLSGG
                     AVARSMGIPDIGLVTMSESIDRAVQVVSAINIPVIADADTGYGNAVNLVRSVREFERA
                     GVAAIHIEDQITPKRCGHLDGKEVISLSEMAKKLEAALATRSDADFCIIARTDARGVN
                     GFDDAVKRANAFAKLGVDAIFFEAPQSEDELAEIPQRVPNIPLLVNVFKGGKTPMLPM
                     ERLEKMGYRIAIYPSETQRAAIQAMRTALSTLKREGTTESIDASLTTFKDRDKVVGLD
                     DWNKIEREFMALETGKD"
BASE COUNT          533 a          671 c          790 g          557 t
ORIGIN      
        1 gatctggcac ttcggttgga gctaactatc gagccgcttg tcgcgttaag tccacggccg
       61 atgtgatcgc caagcttcgg atagttgaag aagaggcaga tgaatccgct tactggatgg
      121 aactactgat tgaatcggga ctgttgcccg cgagtcggct gtcggaattg ttgcaagaat
      181 ccaacgagat cgttgcgatg acagtagcat cgattaggac actacagaaa cgcaccggaa
      241 gttctccgat caatcggcaa tctaaaatcg aaaatctaaa ataggtaggg gtatgaaaca
      301 gatcatcgga ctacttgtag ttattgtgat tctgacagct tgtagggcga cggtcggggc
      361 tcagccggcg aagcctgagc gcatcggagt gattcacttg ggcgaagtct ttgcgacggt
      421 ggtggatgga ttgcgcgccg gcttgaaaga gttgggcatg caagatggca aacagttcgt
      481 tctcgatgtc cacgatctca agggcgactt gaagtcggcg caaccggttg ccgagctctt
      541 ggagcgcgac aaggtcaagc tgatatttac caacacgact ccggttaccg ctagcgcgtt
      601 caaagcgacc aaaaatattt ccatcgtctt tgccgtgggc agcgatccgg tcgccagtgg
      661 ttacgctaaa tccttcggcc aaccgggagg gcggctcacc ggcatccaat atctagcgcg
      721 cgaccttacc ggcaaacgtt tggagattct caaagagatt ctgcccaagc tgcgccaggt
      781 cgtggccttt tacgatcccg gaaacactat cgccgtcgaa gggctcaagt tggggcgcga
      841 ggaggcgcag cgggtgaatg tcaagctggc tgaacggcag gtaaaatcga tcgaggaact
      901 caacaccggc ctcgatgcgc tcaaggccgg cgagttcgac gcctacctgt acatgcccga
      961 tcccatggtg gtgagccaag caccgcgcat cgtcgaagtg gcgcgggcga agcgattgcc
     1021 gacgatgttt cacgatcagg tggtggtggc gcgcggagca ttggcgagct acggccagag
     1081 ctatttcgag atcggccggc gctcggccaa gtatgtccag cgcgtgctca atggcacgcc
     1141 gccagccgag ctgcgaatcg acaccgtcga tgacattgag ctagcgttca atctcgtcac
     1201 cgccaaacaa ctcggtgtga ccatcccgcc gaatgtgctg gcgcgggcac aaaaggtgat
     1261 tcgctaaagg caacagtaaa aagggcaaaa gacaaaacga acggaggcac attgaaggaa
     1321 agaattgctt tgtgcctgcg ggtgattgtc ggtctgggga acagttggag attacatttg
     1381 aagccgcacg gcaatggtga ttgttaatct ttaccggtct ccagcgccat aaattcccgc
     1441 tcgatcttgt tccaatcgtc caggccgacg actttgtcgc ggtctttgaa tgtcgtcagg
     1501 gatgcgtcga tggactccgt cgtgccttcg cgtttgaggg tgctcagcgc tgtgcgcatg
     1561 gcttggatgg cggcgcgttg ggtttccgac ggatagatgg cgatgcggta gcccattttt
     1621 tccagccgtt ccatcggcag catcggtgtc ttgccgccct tgaagacgtt gaccaagagc
     1681 gggatgttgg gcacgcgctg gggtatttcg gcgagttcat cttcagactg cggcgcttcg
     1741 aagaagatgg catcgacacc cagtttggca aacgcattgg cgcgtttgac cgcgtcgtcg
     1801 aagccgttga cgccgcgggc gtcggtgcgg gcgatgatgc aaaaatcggc gtcgctgcgc
     1861 gtcgccagcg ccgcttcgag ctttttggcc atctcggaca gcgaaattac ttctttgccg
     1921 tcgaggtggc cgcagcgctt gggcgtgatc tgatcttcga tatggattgc cgcgacgccg
     1981 gcgcgttcga actcgcgcac gctgcgcacc agattgaccg cgttgccgta gccggtgtcg
     2041 gcatcggcga tgaccggaat gttgatggcg gagacgactt gcacggcgcg gtcgatggac
     2101 tccgacatcg tcaccaggcc gatatcggga atgcccatgc tgcgggcgac ggcgccgccg
     2161 ctcaagtagg cggcttcaaa gccggcttcc tggacgagcc gggcggatag gccgtcgaaa
     2221 cagccgggcg cgacgagcaa tttgttgcgc ttgagtagtt gtttgagagt ttgtctcgca
     2281 gtcatggcaa tcctccgcag tgttttttcg tcctcttcct gatgcgcttg caaagacgtg
     2341 gcggcgacgg caactccccg cgcccttgga agatcctaag ctgatacggg taatgcatcc
     2401 atcgattatt gcgctgatgc catcgcgaac atatcggtga atttgtctat cggcgtggga
     2461 tcgagcgatt gcggttcgct gcacaatttc atgatcgctt cataccgggc gggggcaaag
     2521 cgcgacgcca ggttcgcctc gaatttcttc a
//
LOCUS       DASQKS010000063         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_34953, whole genome shotgun sequence.
ACCESSION   DASQKS010000063 DASQKS010000000
VERSION     DASQKS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34953"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VE170_01635"
     CDS             complement(<1..1596)
                     /locus_tag="VE170_01635"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYJ14158.1"
                     /translation="MAATKTKYIFVTGGVVSSLGKGLAAASIGALLESRGLKVTILKM
                     DPYINVDPGTMSPYQHGEVFVTDDGAETDMDLGHYERYVSTPMGRKNNCTTGQIYETV
                     IGKERRGDYLGGTVQVIPHITDEIKRRIFTAAEGFDIAIGEIGGTVGDIEGLPFLEAV
                     RQVAWDLGRENVLYIHLTLVPYITGGGELKTKPTQHSVKELTGLGIQPDILLCRTDRI
                     LDKKIKNKIAHFCNVAENAVITAKDVEWIYEVPLVFHEEGLDERVVEKLNIWTGAPHL
                     ERWQKVVDVLRNPKDSIRIAVVGKYMSLKESYKSLFESLVHGGIANEVKVEIECIEAE
                     QIEQEAAEILLKNVDGVLIPGGFGDRGSEGKIAAIRYARENGIPFFGICLGMQMAVVE
                     FARNVCGLERANSTEFDLQTTHPVIHLMEEQKAIDTKGGTMRLGSYPCVLQNETLAAK
                     LYGREKISERHRHRYEFNNNYRAEFAAKGMVLSGMSPDGALVEIVELRDHPWFLGCQF
                     HPEFKSRPMDSHPLFRGFVEAALQ"
     gene            complement(1606..2379)
                     /gene="kdsB"
                     /locus_tag="VE170_01640"
     CDS             complement(1606..2379)
                     /gene="kdsB"
                     /locus_tag="VE170_01640"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYJ14159.1"
                     /translation="MSVVAIIPARFRSTRLPGKPLAQIAGRPMIQHVYESAVRAQVLD
                     RVIVATDDRRIEAAVKEFGGEVMMTSPDHASGTDRLAEVARKIKADLLVNVQGDLPFI
                     SAKTIVQAVQPLRRNRDIPMGTVCTAIYDASEWRNPNTVKVLMDKGRMALFFTRAPIP
                     YNRNDRVDPTGNKVDGREQRRLWGYRHLGLYVYRRDFLLKFARLRPTALEQIESLEQL
                     RALENGYRIYVAQVDERSVEVDTPADLLRAENYLKRRSN"
BASE COUNT          597 a          806 c          705 g          602 t
ORIGIN      
        1 ttgcagcgct gcctcgacaa agccgcggaa cagcgggtga ctgtccatgg gccgcgattt
       61 gaactccgga tgaaactggc agccgagaaa ccagggatgg tcgcgcagtt cgacaatctc
      121 caccagcgcc ccatccggcg acatcccgct taacaccatc cctttggcgg cgaattcggc
      181 ccggtagtta ttgttgaact cgtagcgatg gcggtgccgc tcggaaatct tttcccgacc
      241 atacaacttg gcggcgagag tttcattctg cagcacacaa ggatagctgc ccaagcgcat
      301 ggtgccgcct ttggtgtcga tggctttctg ctcttccatg agatgaatca ccggatgggt
      361 cgtctgcagg tcgaattccg tcgagttggc gcgctctaaa ccgcaaacgt tgcgggcaaa
      421 ctccaccacc gccatttgca ttcccaggca aatgccgaaa aatgggatgc cattttcgcg
      481 ggcgtaacgg atcgcggcga ttttcccttc gctgccacgg tcgccgaaac cgccgggaat
      541 gagcacaccg tcgacgtttt tcagtaagat ctcggccgct tcctgctcga tctgttctgc
      601 ttcgatacat tcgatctcga ctttcacctc attggcgata ccgccatgaa ccagcgattc
      661 aaaaagactc ttgtacgact ccttcaaact catgtacttg ccgacaacgg cgatgcgaat
      721 cgagtccttg ggatttctca gcacgtcgac gactttttgc cagcgctcca ggtgcggcgc
      781 gccggtccag atgttgagct tttcgacgac ccgctcgtcg agcccttcct cgtgaaacac
      841 caacggcact tcgtaaatcc actcaacatc cttcgccgtg atgacagcat tttcggcgac
      901 gttacaaaaa tgggcgatct tatttttgat ttttttgtca aggatacgat cggtgcgaca
      961 gagcagtata tcgggttgaa tccccaaacc ggtcagctct ttgacgctat gctgggtcgg
     1021 cttggttttg agctcgccgc cgccggtgat gtaaggcacc agagtcaggt gaatatagag
     1081 aacattctcc cggcccaagt cccaggccac ttgacgcacc gcttccaaga acggtaggcc
     1141 ctcgatatcg ccgaccgtgc cgccaatttc accgatggcg atatcgaagc cttcggcggc
     1201 ggtaaagatg cgccgtttga tctcgtcggt aatatgcgga atgacctgga cagtgccgcc
     1261 gagatagtcg ccgcgccgtt ctttgccgat aaccgtttcg taaatctgcc cggtcgtgca
     1321 gttgtttttc cgccccatgg gggtggatac ataacgttcg tagtggccga gatccatgtc
     1381 ggtctcggcg ccgtcatcgg tgacgaacac ctcgccgtgt tgatacgggc tcatggtgcc
     1441 cggatcgacg ttgatgtacg gatccatctt caagatcgtc accttgagac cgcggctttc
     1501 caaaagcgcg ccgatggaag ccgccgccaa ccctttgccc aaagatgaca ccacaccgcc
     1561 cgtgacgaaa atgtatttag tttttgtcgc cgccatcgcc acctctcagt tcgaacgtcg
     1621 cttcaaataa ttctccgccc gcaggagatc ggccggcgta tccacctcca ccgatcgctc
     1681 gtcaacctgc gcgacgtaga tccgataacc attttccaaa gcccgtaatt gttccaaact
     1741 ttcaatctgc tccaacgccg tcggccgcag ccgggcgaat ttcaataaga aatcccgccg
     1801 ataaacatat aagccgagat gacgatagcc ccaaagccgg cgctgttcgc gcccgtcaac
     1861 cttgttaccg gtcgggtcga cacggtcatt gcgattataa ggtatcggcg cacgggtaaa
     1921 aaacaacgcc attctgccct tgtccatgag tactttgacg gtattcggat tgcgccattc
     1981 gcttgcatca taaatcgccg tgcagactgt gcccatgggg atgtcgcgat tacgccgcaa
     2041 cggctgcacc gcctggacga tcgtcttagc gctaataaac ggcaagtccc cttgcacgtt
     2101 gactagcaag tcggccttga tcttacgcgc cacctcggcc aatcgatcgg tgccgctggc
     2161 atgatcgggc gaggtcatca tgacttcgcc gccgaattct ttaaccgccg cctcaatgcg
     2221 ccgatcatcg gtggcgacga tcacccggtc cagcacttgg gcccggaccg cgctctcgta
     2281 gacatgttgg atcattggcc gtccagcgat ttgggctaac ggctttcccg gcagccgggt
     2341 cgatctaaag cgggccggga taatcgccac cacgctcatg gatcacgaat tcacggatgc
     2401 aatgacaatc gaatgaatgg ttaatgtaga catggcgtcg ctaaaaacaa aaaaccgcaa
     2461 tggctttacg aaaaagcgca tcacggaatt caatactacg ccggcgcata aaagctgtca
     2521 atgcgaattg cccgatcatg cgccccgagg gaaaactttt gccgcgccaa agcctcgatc
     2581 cttcagcgct tgactggtcg cggcacagtc atgataggaa tgaggcaagg tctgtagaaa
     2641 ggacattgcg catggcagaa ttaatcaaaa tcaaagcggg cttcgccacc gtcggccagg
     2701 gcgccgggcc
//
LOCUS       DASQKS010000064         5652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_457319, whole genome shotgun sequence.
ACCESSION   DASQKS010000064 DASQKS010000000
VERSION     DASQKS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457319"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1075)
                     /locus_tag="VE170_01645"
     CDS             complement(158..1075)
                     /locus_tag="VE170_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14160.1"
                     /translation="MNLPETIKLLQFRAAYNLPVHAGVESGIFARHGLRIESAYTPGS
                     LFLSEALRAGKCDIGHTGADDVIADVESTDGSDLFIFMGLHPGLFSLVGAPDHPTVDS
                     LRGKSIGVDAKTSGLVMVLIKLLQDRGISRDNYALIEVGGWQSRYESLIDGRISATLL
                     TEPFVENALNLGGHLLARDFEMIATYQGTCGAASRSWAERNPDRMIRYIRAYVEATEW
                     CFASENRLACLDMLGRHNDIQGSVAEHTLDALLDPRHGLYPRAAVNLPGVLATIELRA
                     DLGYMASPIPPVEKYIGLSYYRSALAIGG"
     gene            1197..1583
                     /locus_tag="VE170_01650"
     CDS             1197..1583
                     /locus_tag="VE170_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14161.1"
                     /translation="MPLKVMRIEDAPIKLMRWDRGKALRLVDEADGAKNVDVHINLIN
                     VDSGPGPTHYHAKAENIYVVLEGNVEAIVEGKKYRLGPGEVAFIPPGVRHQAGNCGDK
                     IARVLEIYAPAGEDFHIVDDSENQPR"
     gene            1600..2556
                     /locus_tag="VE170_01655"
     CDS             1600..2556
                     /locus_tag="VE170_01655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYJ14162.1"
                     /translation="MQLGVLLPHFGKTASPARIIDGGKMCEELGFDSLWVRDHLIWNP
                     HGMERAGLRFVEPFVTLSAVAAVTRKITLGTAVLVPVRWPLKLSQELASLSYLAGGRV
                     IAGIGLGFNPEELATVGMRLEDRVDIMRETVEIARLIWNEDNVSYKGKVFSFDNVTIE
                     PKPVAPIPIFGGGSTRAAIRRVAEYCDGWIPGRVPLATFDDRVKYLRQITDRKIVLGN
                     IPITRIEKDRRKARDSVDVAALAESSEGSSHWIKPRSGKFQTIEDLEGLLIVGNPDDC
                     AAEIEKYRARGVEHLVFDLRLQYDRFEESLELISKEILPRFR"
     gene            complement(2821..3294)
                     /locus_tag="VE170_01660"
     CDS             complement(2821..3294)
                     /locus_tag="VE170_01660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYJ14163.1"
                     /translation="MRFENRGKKNYLDDLMTIHVTNITSYRVIYGDTDQMGVVYYANY
                     LRWFERGRSEFLRQIGLPYGNIEAAGYHFPVTDVHCRYVQSARYDDVVEIATTLVELS
                     RVYLSFEYKISRQADQLTLATGSTKHACIDREGQLKRIPQMLADAIELAKQNLSG"
     gene            3404..3883
                     /locus_tag="VE170_01665"
     CDS             3404..3883
                     /locus_tag="VE170_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14164.1"
                     /translation="MNKLVQRVSWSGGAMLAGLIMFACSSVDDKPAAPAAKPAAPAVA
                     PKPAPAPAPAPAKADGAKTEAVKATKTKAVEVEGVISAVGQRTITFQMERKGKVREEV
                     VGVDEKTKIEKAGANVKLSDLAATDKGMINYEPDAYTPALGIKITGKGEIQKVGGDD"
     gene            complement(3880..4782)
                     /locus_tag="VE170_01670"
     CDS             complement(3880..4782)
                     /locus_tag="VE170_01670"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HYJ14165.1"
                     /translation="MNGHIGLIHSIVPATVDLQVTIPESHPSARNLGPERVGSGTIID
                     PSGYILTVHYVTIGAASIIVTLPDGEEYPGQVVGQDQESGLSLVKIPGHDLPFLKMAA
                     ADSLALGQAVVMVSSSGKVGRRVSGGYVSSLAGYDGEWEYMIDKSIRVTAFNPGFGGG
                     TLANFKGELAGIVSLNLNEVGKFTMAIPVEYYQTYEQELKQHGQVRSRPRRPWLGIYT
                     QGVAGLVVIGNVAGNGPAAKYGLKAGDVILSVENNKIRSRQELYHEMWKKHPGERILF
                     RVIRDDEAVDVVVVSGDRADRYRT"
     gene            5138..>5652
                     /locus_tag="VE170_01675"
     CDS             5138..>5652
                     /locus_tag="VE170_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HYJ14166.1"
                     /translation="MKISIDEIPQSPKEIAFSESVEGLNKIYSQTSVREFGFPTSLEV
                     ELRYYRTAADLFFDGRLSGQLRGVCGRCAEEYKFDLDQPFEFVLIPAAAKAGRKAEEL
                     HRDDLALSYYSGDEVDLSPLIAEQVILALPTRPLCDDSCRGLCGVCGINLNLEACSCA
                     SPTGDPRMAVFR"
BASE COUNT         1270 a         1517 c         1626 g         1239 t
ORIGIN      
        1 gacgcgttgc agcactgttt gcaaatcgaa accgacgacg cggtgtatgc cgagatcgaa
       61 gcggcgctaa ctcgattgag cgagcctgct gggagagcgc agctcgagta aagtgtattt
      121 gtttgttgcg cttcctagca aacctgtccc tattgtgtca tccgccgatc gccagggcac
      181 tgcgataata ggagaggccg atatacttct ccaccggcgg aatgggtgat gccatatacc
      241 ctaggtcggc tcgtaactcg atcgtggcca gaactcccgg taaattgact gccgctctgg
      301 ggtacagccc gtgccgtgga tcgagcagcg catcgagtgt gtgttcggct acgcttccct
      361 gtatatcgtt gtggcgccca agcatgtcca ggcaagccag gcgattctcg ctcgcgaaac
      421 accactcggt tgcttccacg taggcgcgga tgtaacgaat cattcgatcg gggttgcgct
      481 cagcccaact ccggctcgcc gccccgcagg tgccttggta agtggcgatc atctcgaaat
      541 ctcgcgccag cagatgacca ccgagattca aagcattctc gacgaaaggc tcggttaaca
      601 gagtcgcact tattcttccg tcgattaagg actcatagcg gctttgccag ccgccaacct
      661 cgatcaatgc ataattgtct ctcgaaatgc ctcgatcctg gagcaatttt atcaacacca
      721 tgactaggcc gctggtcttg gcatcgacgc caatggactt gccgcgcagt gaatcgacgg
      781 ttgggtggtc cggcgcgccg accaagctga aaagacccgg atgaagcccc atgaagatga
      841 acaagtcgga gccgtctgtg ctttcgacgt cggcgatcac gtcgtcggcg ccggtgtggc
      901 cgatgtcgca tttgccggct ctcaacgctt cgctaagaaa cagagagcca ggcgtgtagg
      961 cggattcaat gcgaagcccg tggcgggcga aaattccgct ctcgaccccc gcatggaccg
     1021 gcaggttgta agcagcgcga aactgcaaaa gtttgatggt ctcgggtaaa ttcatagtgc
     1081 cgcgtctcga tctgctccta tcacggcgcg cggtaacgtt aaacccatga gcgcgttcaa
     1141 cttgacaaag tgcggggcat cccagtagtg atcaataaac aattggaggt gaactcatgc
     1201 cgttgaaagt gatgcgaatc gaagatgcac cgatcaaact gatgcgctgg gaccgcggca
     1261 aggcgctccg tctggtggac gaagcggacg gcgcgaagaa cgtcgacgtg catatcaatc
     1321 tgatcaatgt cgactcgggg cctggcccga cgcactatca cgccaaagcg gaaaatattt
     1381 acgtcgtcct ggaaggtaac gtcgaggcga tcgttgaggg gaaaaagtat cgcctcggtc
     1441 ccggcgaggt cgcctttatc ccgcctggcg tacgccacca ggccggcaat tgtggcgaca
     1501 agattgcccg agtgctggag atttatgctc cggccgggga ggattttcat atcgtcgatg
     1561 attcggagaa tcaaccgcgc tgagtgacta accgcgaaaa tgcaattggg cgttttattg
     1621 ccgcacttcg gcaaaacagc ttcgccggca cgtattatcg acggcggtaa gatgtgcgag
     1681 gaattgggat tcgattcgct ctgggtgcgc gatcacttga tctggaaccc gcacggcatg
     1741 gagcgggccg gcttgaggtt cgtcgaaccc ttcgtcacac tttcggcggt cgcggccgtg
     1801 acgagaaaaa taaccttggg caccgcggtg ctggtgccgg tgcgctggcc gctcaaactc
     1861 agccaagagc tcgccagtct ctcctatctc gcgggcggcc gggtgatcgc cgggatcggt
     1921 ttgggattca atcccgagga actcgccact gtcggaatgc gccttgagga tcgtgttgac
     1981 atcatgcgcg agaccgtcga aatcgcgcgc ttaatctgga acgaagataa cgtgtcctat
     2041 aagggcaagg tgttttcgtt cgataacgtc accatcgaac ccaagccggt tgcacccatc
     2101 ccgatcttcg gtggcggcag cacgcgcgcc gcgatcagac gagtggccga atactgcgac
     2161 gggtggattc ccggccgcgt gccgttagcg actttcgacg accgggtaaa atatttgcgg
     2221 cagatcaccg atcggaaaat tgttctgggc aatattccca tcacccgtat cgagaaggat
     2281 cgtaggaaag cgcgcgatag cgtcgatgtc gccgccctcg ccgagagttc ggaaggaagc
     2341 agccattgga tcaaacctcg gtcgggaaaa tttcaaacca tcgaagatct ggaaggtttg
     2401 ttgatcgtcg gcaaccccga tgactgcgcc gccgagatcg aaaaataccg cgcgcgaggc
     2461 gtcgagcatt tggttttcga cttaagactg caatacgatc gcttcgaaga gagtttggag
     2521 ttgatcagca aagaaattct gccgcggttt cgttaaagac tggcgctgcg atgaaatcca
     2581 atgagaaaga ataacacgtg tttccccctt ctcccggctc gccgaaactc ctggccgacg
     2641 ttgttgtcag catcctgatt taatgaggtg ctggagcctg tgccgcatcg cgtgacacgc
     2701 catgatggtt agctccatta tcgacttgcc ccgaggtctt tcatcgtaga acaagacccc
     2761 accttgccga aggggatgcg ggaaactcgc ttttgcccgt gggcagagcg aagctgcacc
     2821 ctacccagat aggttttgtt tcgccagctc gatcgcgtcg gcgagcattt gggggattcg
     2881 tttcaactgg ccctcacggt cgatacacgc atgtttggtc gatccggtgg ccagggtgag
     2941 ttgatcggct tggcgggaaa ttttgtactc aaacgacaga taaacccggc ttagctcgac
     3001 caaggtcgtg gcgatttcga cgacatcgtc gtaacgcgcc gactggacat agcggcagtg
     3061 aacgtcggtg acgggaaaat ggtagccggc ggcttcgata ttgccgtagg gcaaaccgat
     3121 ctggcgcagg aactccgatc tgccgcgttc aaaccaacgc agataattgg cgtaatagac
     3181 aacgcccatt tggtcggtgt cgccgtagat cactcgatag ctcgtgatgt tcgtcacgtg
     3241 aatcgtcatg agatcatcaa ggtagttctt tttgccgcga ttctcaaagc gcattggcct
     3301 gaccgccttg acaatctaaa cggaagtatg ttttagctga ggaaattgcg gcaatttata
     3361 ttgcgcgata ttttagattt tttgggcatc aggaggaaag ggaatgaata agctagttca
     3421 gcgtgtatct tggtccggcg gcgcgatgct cgcaggattg ataatgtttg cttgcagctc
     3481 cgtggacgac aaacccgctg cgccggcggc gaaaccggcg gcacctgcgg ttgcacctaa
     3541 accagctccg gctccagcgc cggctcccgc caaagccgat ggtgcaaaga ccgaggcggt
     3601 gaaggcgacc aagaccaaag ctgtcgaagt tgaaggcgta atcagcgccg tcggtcagcg
     3661 gacgattact ttccaaatgg aacgcaaggg taaagtgcgc gaagaggtcg tcggcgtcga
     3721 tgaaaaaact aaaatcgaaa aagccggcgc gaacgtcaag ctgagcgatc tcgctgccac
     3781 cgacaaaggg atgatcaatt atgagcccga cgcgtatacc ccggcgctcg gaatcaagat
     3841 taccggcaag ggtgagatcc agaaagtcgg cggcgacgac taggtgcgat agcgatcggc
     3901 gcggtcgccg ctgaccacga cgacatcgac tgcttcatca tcacgaataa cccgaaacaa
     3961 gatccgttct cccggatgtt ttttccacat ctcgtggtaa agctcctggc gggaacggat
     4021 cttgttgttt tccaccgata agatcacatc gccggccttg aggccgtatt tcgccgccgg
     4081 tccgttgccg gcgacattgc cgatgaccac cagaccggcg acgccttgag tataaattcc
     4141 cagccaaggt cggcgcggtc ggctacgcac ctggccatgt tgtttaagct cctgttcata
     4201 ggtctggtag tattccacgg ggattgccat ggtgaatttg cccacctcgt tcaagttgag
     4261 cgagacgatg cccgccagct cacctttgaa gttggccaaa gtgccgccgc caaaaccggg
     4321 attgaacgct gtcacccgga tggatttatc gatcatgtat tcccattcgc catcgtagcc
     4381 ggctaaagaa ctcacatagc cgccgctgac ccgccggccg accttgccgc tgctggagac
     4441 catcactacc gcttgtccga gcgccagcga atccgctgcc gccattttga gaaagggcaa
     4501 atcgtgtccg ggaattttaa ctaacgacag gccagactct tggtcttgcc cgacaacttg
     4561 tcctgggtat tcctcaccgt cgggtagggt aacaatgatc gaggcggcgc cgatggtgac
     4621 gtaatgaacc gtgagaatgt aaccgctggg atcgatgatg gttccggatc cgacccgttc
     4681 cgggccgaga ttgcgcgccg aggggtgact ctcgggaata gttacttgca gatcgaccgt
     4741 tgccggtacg atcgaatgaa tcaatccaat atggccgttc aaagtactgc tccttcactg
     4801 attacatagg ctaggctatt ccgaaactcg ggctttgtat agagatccac cgctgcgcag
     4861 caaacaatta aaaaaatcgt cgcgctcgcc acgccgaagc tcgtgaaggt tgggataatt
     4921 catgggccgg acaaaaaagg gttaacaaaa tggcgatgaa ttcacaagcc gattctgcgc
     4981 cctgcgagtt ttgcggcgag gcctggcgcg actccatagt tatcgtggaa gtgctgaata
     5041 aatcatgagc ttttgcgtca acgggatgat cactcggctt gacatcgcaa gcgattgccg
     5101 ttatttaata ttaattaatt ggcccgcatt atcgccgatg aaaatctcca tcgacgagat
     5161 tcctcaaagc cccaaagaga tcgcattctc cgaaagtgtt gagggactca ataaaattta
     5221 tagccaaacc agcgtccgtg aatttggctt tcctacgtcc ttggaggtcg agcttcgtta
     5281 ttaccgtacg gcggccgatt tgttttttga cggacggttg agtggccaat tgcgcggcgt
     5341 gtgcggacgt tgcgccgaag aatataagtt tgacttggat cagccctttg agttcgtgct
     5401 gatcccggct gcagctaaag ccgggcgtaa agccgaagag ctgcatcgcg acgatttggc
     5461 attgagctat tattcgggcg acgaggtcga tttatcgccg ctgatcgccg agcaggtgat
     5521 actggcgctg ccgacgcggc cgttgtgcga cgatagctgc cgcggcctgt gcggtgtttg
     5581 tggtatcaat ctcaaccttg aggcctgctc ctgcgcgtcg cccaccggag atccgcgcat
     5641 ggcggtgttc cg
//
LOCUS       DASQKS010000065         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4235739, whole genome shotgun sequence.
ACCESSION   DASQKS010000065 DASQKS010000000
VERSION     DASQKS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235739"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VE170_01680"
     CDS             complement(<1..251)
                     /locus_tag="VE170_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ14167.1"
                     /translation="MGFTEKIARFIVDTDYADIPRDAVDKAKRSALDCVGAALAGVAE
                     PVSQTITGYVTKLGGPPQASVFGAGLKAAVADAALANGT"
     gene            complement(295..564)
                     /locus_tag="VE170_01685"
     CDS             complement(295..564)
                     /locus_tag="VE170_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HYJ14168.1"
                     /translation="MLPSVTKAKYVKDYLIEVKFNDGTKKVIDFEPWLSGPIFRPLKS
                     KVYFKKFFIDGPTIAWSNGADIAPETLYGAPAVKLNNELKLRRAG"
     gene            complement(573..833)
                     /locus_tag="VE170_01690"
     CDS             complement(573..833)
                     /locus_tag="VE170_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HYJ14169.1"
                     /translation="MPTICVFFGIIIRMFYRDHNPPHFHAEYQGQNATFDFDGKLLEG
                     DIESRTAKDLIRKWARRHKTELNKNWEHMRQKRPFTRIEPLK"
     gene            complement(1087..>2504)
                     /locus_tag="VE170_01695"
     CDS             complement(1087..>2504)
                     /locus_tag="VE170_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14170.1"
                     /translation="DRDTILKSFERVEAITLPALRAGVKWGFTTFGQYLDAMRGNLGV
                     NVASLVGHCALRQYVMGEASIERAATPAEIHQMKEVLKASVRAGAAGFSTNQNPVHMY
                     ADGTPIQSRFATDEEIIELACALGEINQGAVQISRGSLGVSVPMRESVKLFEEISSRS
                     GRPVIWQSITHRWDKPNEWRELLDLAKETIDRGVQSYPLCNARLFNNRLTMKNAQVFD
                     DLPTWKTILFLPLEERIKAIKDPETRKKMRYEAVEETKSSRFSRRWDLVYLINAVKPE
                     NKHLEKKSVAEIAKIRNQDIIDAFLDLSLEEGLDTEFQTSSTNGDEKAVAEIIKSPYV
                     LVGQSDAGAHLIYDAGFGYATRLLGYWVREKQIMTVEEAVRKLTFMVASIFGLPDRGL
                     LRRGMAADLCLFDPATVRECDAEMVQDLPGNEKRFIQKAIGIEMTVVNGQVLVEKESH
                     TGALPGTVLGGGNGAALQAAA"
BASE COUNT          484 a          707 c          686 g          627 t
ORIGIN      
        1 atggtgccat tggccagggc agcgtctgca accgcagctt tgagaccagc gccaaatacc
       61 gatgcctgag gcggcccgcc gagtttggtt acataacctg tgatggtttg actgaccggc
      121 tcggcaacgc cggccagggc ggcgcccacg cagtcgagcg cagagcgctt ggccttatct
      181 acggcatcgc gcggtatgtc cgcgtaatcg gtgtcaacga tgaagcgggc aattttttca
      241 gtaaaaccca tgattaacct ctttgagaaa tgcaatggga actgcgtggg tgatctatcc
      301 cgcacgccta agtttcaatt cgttgtttaa cttgactgcc ggcgcaccat aaagcgtttc
      361 cggggcgatg tcggcgccgt ttgaccacgc gattgtcggg ccgtcgataa aaaatttttt
      421 gaaataaacc ttgctcttca agggtctgaa tatcggtccg gatagccacg gttcaaagtc
      481 gatcaccttc ttcgttccgt cattgaactt cacttcgatc aggtagtctt taacgtattt
      541 agcttttgtt actgaaggca gcatctgtgt tcctatttca atggttcgat tctcgtgaac
      601 ggtctcttct gccgcatgtg ttcccagttc ttgttaagtt ctgttttatg gcggcgtgcc
      661 cacttcctga taagatcctt agcagttcga gactcaatgt ctccttcgag cagcttcccg
      721 tcgaaatcga atgttgcgtt ttgtccttgg tattccgcat ggaagtgcgg cgggttatgg
      781 tcccggtaga acatgcgaat tattatacca aagaatacgc atatggttgg catacacaga
      841 gagcgagaga ggaatttaga attattcgcg cagtaaaact ctccggtgag ttaggtgctt
      901 gctgtcagcg gctgcgaacc atctcaactg aatttcagcc gacatgcagt tgggacgact
      961 atcggtcgcc ccaactgttt tgcatatttt ttgcgcgacg caagtttctt tgctcagaac
     1021 aagctttgcg gctaatttcc cttccctttg cccatgtgac ctttgtgcgc tttgtggtta
     1081 aattctttaa gccgccgcct gcagcgctgc gccgttgccg ccaccgagca cagttccggg
     1141 caacgccccc gtgtgacttt ccttctccac cagcacctgg ccgttgacga cggtcatctc
     1201 gatgccgatc gctttttgga taaagcgctt ttcgttgccc ggcaagtctt gcaccatctc
     1261 ggcgtcgcat tcccggacgg tcgccgggtc gaacaaacac aggtccgccg ccatgccccg
     1321 gcgcagtaag ccgcggtccg gcaaaccgaa gatcgaggcg accatgaagg tcaacttgcg
     1381 cacggcttct tccaccgtca tgatttgctt ctcacgcacc cagtaaccca agagccgtgt
     1441 cgcgtagcca aaaccggcat cgtaaatcaa atgcgcgccg gcgtcggact ggccgaccag
     1501 tacgtacggg cttttgatga tttccgccac cgctttctcg tcgccgttgg tgctcgaagt
     1561 ttggaactcg gtgtccaagc cttcttccaa agataaatcg aggaaggcgt cgatgatgtc
     1621 ttgattacgg atcttggcga tctcggcgac gctctttttc tccaggtgct tgttttccgg
     1681 tttgacggca ttgattagat agaccagatc ccagcgccgc gagaagcgcg acgacttggt
     1741 ttcttcgacc gcttcgtagc gcattttctt gcgcgtctcg gggtctttga tcgctttgat
     1801 gcgctcttcc aacggcagaa acaggatcgt cttccaggtc ggcagatcgt cgaacacttg
     1861 cgcgttcttc atggtcagcc ggttgttgaa caggcgcgcg ttgcacagcg gataagactg
     1921 cacgccccgg tcgatggtct ctttggccag gtccaatagt tcgcgccatt cgttgggctt
     1981 gtcccagcga tgggtgatgc tctgccagat caccggccgg ccggagcggc tggaaatttc
     2041 ctcgaacaat ttcacgctct cgcgcatcgg caccgacacg ccgagggaac cgcgggaaat
     2101 ctgcaccgcg ccctgattga tttcgccgag cgcgcaggcc agttcgataa tttcttcgtc
     2161 ggtggcaaag cggctctgga tcggcgtgcc gtcggcgtac atatgcaccg gattttgatt
     2221 ggtcgagaaa ccggcggcgc cggcgcgcac gctggctttg agcacttcct tcatctgatg
     2281 gatttccgcc ggcgtggcgg cgcgctcgat ggacgcttcg cccatgacgt attggcgcag
     2341 cgcgcagtgg cccaccaacg aggcgacatt aacgccgaga ttgccgcgca tggcgtcgag
     2401 gtactggccg aaggtggtaa agccccactt cactccggcc cgcaaagccg gcagcgtgat
     2461 cgcttcgacg cgctcgaagc ttttgagaat ggtatcgcga tccg
//
LOCUS       DASQKS010000066         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1716322, whole genome shotgun sequence.
ACCESSION   DASQKS010000066 DASQKS010000000
VERSION     DASQKS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716322"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VE170_01700"
     CDS             complement(<1..900)
                     /locus_tag="VE170_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYJ14171.1"
                     /translation="MKLGMVRLALLAGVIVFGAQNVKAAEDKPLGPGWLSLDSSVGRL
                     DGAIADGKGGLEKALGIGISGFIDTGYTWSSNHPGNGGNISGRYFDKDNNQFNVNNFH
                     LALEKSEKDWGVGFKVSGDFGRYGELLHEATQWNKTGARFCCGPSAELREAYLNTTIP
                     VGEGIGVKGGLFTTLLGTEILNNPGAYNDNISRSFAFNFGVPLRHLGMLFSYPFHKTV
                     TATLGVVTGWDNPHDNNSAPSGIVGVNFMPSDAFGLASNLIVGPEQFHNTGRQRATWS
                     NVATIKPLDPLTILLEYTLGGESS"
     gene            complement(1197..3356)
                     /locus_tag="VE170_01705"
     CDS             complement(1197..3356)
                     /locus_tag="VE170_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HYJ14172.1"
                     /translation="MTTDHKKSPGGGIERWIKSYCPYCGVGCGLLAGIRDGAVAKIKG
                     DPAHPSSRGDLCLKAVYLPEILRTADRLLYPQIRSCQDGPFKRASWDQTMAYLVKRFR
                     DIIDEHGPDAVAFYGSGQLTTEEYYIANKLAKGFIGTNNFDTNSRLCMASAVAGYKTS
                     FGADGPPNSYADIDEANCIFLIGTNTADCHPVLFKRIKQRKQSAPNDVVVICVDPRRT
                     ETADFADLHLPIRPGTDIALLNAMLHIVIDENLIDREFVARHTNGFAELLNNVKQYSA
                     KTVEAICGIPECLIVEAALIFGKSANALSLWSMGINQSTVGVHKNNAINNLHLVTGKI
                     GKPGCGPFSLTGQPNAMGGREAGGLSHMLPGYRSVENDAQRGAVEKFWRRPPGTISTQ
                     PGLPALEQFEALAAGKLKAIWILCTNPAASAPDLDLIEKALRQAELVIVQDAYHPTAT
                     SRLAHVILPAAQWSEKEGAMTNSERRVTYMPKLVDAPGDALPDWQIIARFAAALGFGA
                     DFAYTSSQDIFAEFAALTENTPCDCSGMTYRKLNASPLQWPHPKRSPCDTTHLYADGI
                     FPSSDGRAHLIAVEHAEPAEVTDTDYPLVLTTGRSKYHWHTMTRTGKNAALRKSAPDP
                     ILDLHRADARRYNIHDADFVEVTSRRGKVVVQCRVTEEIVAGTCFLPFHWSRDEGFFK
                     AANNLTNSARDPVSRQPELKACAVRVRQIFDFPLEDN"
     gene            complement(3386..>4047)
                     /locus_tag="VE170_01710"
     CDS             complement(3386..>4047)
                     /locus_tag="VE170_01710"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14173.1"
                     /translation="QVSDRSTHKARKELADNFLNAVGLAGAADSYPDELSAGMRQRVG
                     IARALALEPKVLLLDEPFSLLDVVTRMELQDQLMDLCAQEHKTVLMVTHDVDEAILLA
                     DRIVMMTNGPAATVGEIVTVPFERPRDRLGVAEDPHYHSIRSQLLGFLEERAHGSATT
                     EPPVECQAVVSQNPKGVSQELLTIDGNSGFNRFRAKFRKQPPAADAATSSNRAAAKAR
                     S"
BASE COUNT          883 a         1138 c         1119 g          907 t
ORIGIN      
        1 cgacgattcg ccgccgaggg tatactcaag caaaatcgtc aaaggatcga ggggtttgat
       61 cgttgctacg ttactccagg tcgccctttg gcgtccggta ttatgaaatt gttcgggccc
      121 gacgatgaga ttgctcgcca agccaaaggc atcgctgggc ataaagttca cgccgacgat
      181 accagacggg gcgctgttgt tgtcatgcgg attatcccaa ccggttacca cgcctaaagt
      241 ggccgtgacc gttttgtgaa acggatagct gaacaacatg cctagatggc gcaatggcac
      301 accaaagtta aatgcgaaag aacgagaaat attgtcgtta tatgccccgg ggttgttgag
      361 aatttccgtg cccaagagag tcgtgaacaa gccgcctttg acaccgatgc cttcgcccac
      421 gggaattgtg gtgttaagat acgcctcacg caactcggcc gacggtccgc agcaaaacct
      481 ggcaccagtc ttattccact gtgtggcttc gtggagcagc tcgccatatc gaccaaaatc
      541 gccggaaacc ttgaaaccaa cgccccaatc cttttccgac ttttctagtg cgagatgaaa
      601 attattaaca ttgaactgat tattatcttt atcgaaatag cggccggaga tattaccccc
      661 atttccggga tgattcgagc tccatgtgta gcccgtatcg ataaatccgc tgatgccgat
      721 gcccaaagct ttttccaatc cgcctttgcc gtcggcgatg gcgccatcga gcctgccgac
      781 actactatcc agacttaacc agcctgggcc gagcggctta tcctcggccg ccttgacgtt
      841 ttgcgcccca aacacgatga ctccagccaa gagcgcgagt cgtaccattc caagtttcat
      901 taccgttcct cctcgaaaat cgaattccgt caacctatac aaatccttca ctaaacccaa
      961 ccgcctattc accagattta cacaaatttt acgaatatta tttgcgaaga aacagagcaa
     1021 aacgaatgcc ataaaaaaat ccttccgtca gtgactcgat ggtagatctg acgctacttt
     1081 ttatagattt aaataccacg gattaaaaat tgcctattta ttgacgctgg tgcttaaaat
     1141 ttaatcaagc atgttggctg gcgagaaaag tcctgccgat cacgtcgcgg ccttggttag
     1201 ttatcttcca gaggaaaatc gaaaatttgg cgcacgcgca cggcgcatgc tttgagttcg
     1261 ggctggcgcg acaccgggtc ccgtgccgag ttcgtcaagt tattagccgc cttgaagaat
     1321 ccctcgtctc ggctccagtg aaacggcaaa aaacaggtgc cggcgacgat ctcttcggtg
     1381 acccggcatt ggaccaccac cttgccgcgg cgggatgtaa cttcgacaaa gtcggcgtca
     1441 tgaatattgt aacggcgcgc atctgcgcgg tgcaggtcga gaatcggatc gggggcgctc
     1501 ttgcgcaacg cggcgttttt gccagtgcgc gtcatggtgt gccagtggta tttcgatcgt
     1561 ccggtggtca gcactaatgg ataatcagtg tcggtgactt cagccggttc ggcatgctcg
     1621 acggcaatca agtgggccct gccgtcgctc gacggaaata ttccatcggc atataaatga
     1681 gtcgtatcgc aagggcttcg tttcggatgg ggccactgca aagggctggc gttcagcttg
     1741 cggtaggtca tgcccgagca atcgcagggg gtattctcgg ttagggcagc gaactcggca
     1801 aagatatctt gcgacgaagt gtaagcgaaa tcggcgccga aacccaaggc cgccgcgaac
     1861 cgcgcgatga tttgccagtc cggcagcgcg tcgccgggcg catcgactaa cttcggcatg
     1921 taagttacac gccgttcgga gttggtcatt gcgccttctt tttcactcca ctgggcagcc
     1981 ggtaggatca catgagccaa acggctggtg gccgtcggat gatacgcgtc ctgaacaatc
     2041 accagctcgg cttgccgcaa cgctttctcg atcaagtcga gatcgggcgc cgatgctgcc
     2101 ggattggtgc aaagaatcca gatcgctttt aactttcctg ctgccaacgc ttcgaactgc
     2161 tcgagagcgg ggagaccggg ctgcgtcgag atggtacccg gtggccggcg ccagaatttt
     2221 tcgaccgcgc cgcgctgcgc gtcgttctcg acgctgcggt acccgggcag catgtgcgac
     2281 aggccgccgg cttctcggcc ccccatggcg ttgggctggc cggtgagcga gaacggccca
     2341 cagccaggct tgcctatctt accggtgacc agatgcaaat tattgatcgc gttgttcttg
     2401 tgcacaccga cagtgctctg attgatcccc atgctccaga gcgacagcgc gttggccgac
     2461 ttgccgaaga tcagcgccgc ctcgacgatc aaacactcgg ggatcccgca gatcgcttcg
     2521 acggttttcg ctgagtattg cttaacgttg ttgagtaact cggcaaaacc gtttgtgtgg
     2581 cgggcgacaa actcgcggtc gatgagattc tcatcgatca caatatgaag catagcgttg
     2641 agcagcgcga tgtccgtccc cggacgaatc ggtaaatgca aatctgcgaa gtctgcggtc
     2701 tcggtgcgcc gcggatcgac gcagataacc acaacatcgt tgggcgcgct ctgcttgcgt
     2761 tgcttgattc gcttgaaaag caccggatgg cagtccgcgg tgttggtgcc gataagaaag
     2821 atgcaattgg cttcgtcgat atcggcatat gagttgggcg ggccatcggc gccgaacgaa
     2881 gttttgtaac cggccaccgc tgaggccatg cacagacgag aattggtatc gaagttgtta
     2941 gtgccgatga atcctttagc caacttgtta gcgatgtaat actcttctgt agtcaattgc
     3001 cccgagccgt aaaaagccac agcatccggc ccgtgctcgt caataatgtc gcgaaaacgt
     3061 ttgaccaaat aggccatagt ctgatcccag gacgcccgct tgaacggacc gtcttggcag
     3121 gaacgaattt gcggatacag caagcgatcc gcagtgcgca agatctcggg caggtagacc
     3181 gctttgaggc aaagatcgcc acggctcgac ggatgcgccg gatcgccttt gatctttgcc
     3241 accgcgccat cgcgaattcc ggccaagagc ccgcagccga cgccgcaata cggacagtag
     3301 cttttaatcc agcgttcgat gccgccgccc ggcgattttt tgtggtcggt agtcatgccg
     3361 catgactcgg ctcattctga tcggatcatg accgcgcctt ggctgcagcg cggtttgaac
     3421 tcgtggccgc atccgccgcc ggcggttgct tgcggaattt ggcgcggaag cgattgaacc
     3481 cggaatttcc atcgatcgtc agcagctctt gcgagacccc tttcggattt tggctgacga
     3541 ccgcttggca ctcaactggc ggctcggttg tcgcgctgcc atgcgctcgc tcctcgagaa
     3601 accccagcaa ttggcttcgt attgaatgat aatgcggatc ttcggcgacg ccgagccggt
     3661 cgcgcggccg ttcgaacggt accgtgacaa tctcgcccac cgtggccgcc ggcccattgg
     3721 tcatcatgac aatgcggtcg gcgagcaaaa tcgcttcgtc gacgtcgtgg gtcaccatga
     3781 gcaccgtctt atgttcttga gcgcacaggt ccatcaattg gtcttgcagc tccatgcgcg
     3841 tgaccacgtc gagcaagcta aacggctcgt cgagcaacaa cacctttggc tccaatgcca
     3901 aggcgcgcgc gatgccgacg cgctggcgca tgccggcgga aagttcgtcg ggatagctgt
     3961 cggcggcgcc agctaaacca acggcgttaa gaaaattatc ggcgagctct ttgcgcgcct
     4021 tatgcgtgga ccgatcgctc acctggt
//
LOCUS       DASQKS010000067         9993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5922768, whole genome shotgun sequence.
ACCESSION   DASQKS010000067 DASQKS010000000
VERSION     DASQKS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5922768"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VE170_01715"
     CDS             complement(<1..782)
                     /locus_tag="VE170_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14174.1"
                     /translation="MLHLFRFVVAIVIGSLSLIAPVAAQPLEKLRLVYSAIGGSQASF
                     WVPYEAGIFRKHGLDVELLYVAGGGRAAQVVQSGEVPIGVFTGGAVINSNLAGGDLVV
                     IASSMNVMTFVVMARPEIRRVEDLKGKKIGVSRFGSATDFGLRYAEEQWPIKRQRDFA
                     VLQMGGVTDVYNALRAGVLDAAVINAELAILGRREGFRELADIAKMGVHFPTSSVITT
                     RSYIKRNENTVRKFIRGFVEGVHYGKTQRAPTIKIMMKYLRS"
     gene            complement(1131..1802)
                     /locus_tag="VE170_01720"
     CDS             complement(1131..1802)
                     /locus_tag="VE170_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYJ14175.1"
                     /translation="MIKALIFDFDGIIIDSESPELLAWQEVFIAHGRELDLEVWADLV
                     GRPSNHFDLYGYFNEHIDPTADIATLRLNRRARVIALTEQQPVLPGVQEYLRGAGDLG
                     LKLGIASSSSANHVRGHLQRLDLLRYFDTTKCFEDTTTHKPDPAPYRAVLDTLGIAPH
                     EAVAFEDSPNGVTAARAAGIFCVAVPNPVTCRLVLDHADHRLASLADEALEQVLMRAL
                     AKTTS"
     gene            complement(1980..2378)
                     /locus_tag="VE170_01725"
     CDS             complement(1980..2378)
                     /locus_tag="VE170_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14176.1"
                     /translation="MLSAVFLVLSAILILVSLRRRVVVRRRAKGTDWALETAFIRLDF
                     PAAHRAVAQQLATGLAEVVGMKIKQLTPDHTLAQIADWADDRIHAKDLITLFVVAFSV
                     RCDSNTSFRDLVEKVAEKKAADGNDGRVMN"
     gene            complement(2454..3092)
                     /locus_tag="VE170_01730"
     CDS             complement(2454..3092)
                     /locus_tag="VE170_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HYJ14177.1"
                     /translation="MYLPGHFEEKRIEVLHQLMREHPLATLVTLGADGISANHLPIEV
                     DAAPAPNGTLRCHVARANPVWRDHSRETEALVIFHGAQAYISPSWYETKKQTGEVVPT
                     YNYAVVHASGALRIIEDRAWLRGLVTRLTARFESASAAPWHVSDAPPDFIDKQLGAIV
                     GIEIAVTRLAGKWKVSQNRPAQDRAGVAAALGDSVDADSLAMAQMIKERSSS"
     gene            3267..4319
                     /locus_tag="VE170_01735"
     CDS             3267..4319
                     /locus_tag="VE170_01735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14178.1"
                     /translation="MTISRKPTIWAGVLVTLTLLWPSPRLHAQESFFKGKQIRVIVGL
                     STGGGYDRAARLISRYMGKYLPGNPDFAVQNMPGAGSVTAANYLWSVAKPDGLTLLAP
                     HNNFYLAQLSGQKEVKFDLTKFQWIGSLENDDMIMFARADAPFRSVADIIRSKEPPKC
                     GSTGVGSSDYVMSKILEETVGMKVGHVTGYPGSSEIALAMERGEVACMGLTIATYYGR
                     EPFLSWLKTKFVRFLAQSGHKRSPRVADAPTIYELMDEYKTAPTKRRVAEAMLQGGEW
                     ARPLIAPPATPPDRVKILRDGYERASKDPELLAEAKKLSIEIAPLRGEQLQKTASDII
                     KQPAEVTEQIKKLFVQ"
     gene            complement(4407..5615)
                     /locus_tag="VE170_01740"
     CDS             complement(4407..5615)
                     /locus_tag="VE170_01740"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ14179.1"
                     /translation="MLFAKLGLSDKVLEGVKAAGYTDPTPIQLRAIPLALEGRDIIGS
                     AQTGTGKTAAFALPIISRLGQHGKTRALILEPTRELAAQVETAFHDFSRFTDLRTVVL
                     FGGTGYGKQDLALRRGTDVVVATPGRLLDQMQRQMLRLNDIEFLVLDEADRMLDMGFM
                     PDVRKIVDRVPKARQTMLFSATIPPEIDALVRWAMKNPETIQIGQRRSPAETITHALY
                     PVDVAQKQELLTELLERTEYDQVLIFCRTKNGADRVARRLTEKGHAVAVLHSNRTQRE
                     REQALNGFRNGRYEVMVATDIAARGIDVEQISHVINYDVPHHPEDYVHRIGRTGRALS
                     VGDAFTLMTAEDLQEVAAIEHFIGQKIPRLKLENFNYAFTRLLDPNPKPTFGARAGAK
                     RAYGPGRGRW"
     gene            complement(5660..6283)
                     /locus_tag="VE170_01745"
     CDS             complement(5660..6283)
                     /locus_tag="VE170_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HYJ14180.1"
                     /translation="MLAARYLGDRPAVKVERYAAKLARYFPYDPEAPRAAARLIDAIL
                     DYRSTLYVDHIGSTAVPGCGGKGIIDLLVTYPAGAFFIARDALDQLGFQPQSGPEPFP
                     ESRPMRVGVTTYRGKAYRVHAHVIRSGDPEAQNLIKFRDCLRADAELVQAYQVEKLSI
                     LSSGITASTEYSKAKSDFIRAVLGERVRSPRSELTTRAPASRLFTAR"
     gene            complement(6513..6829)
                     /locus_tag="VE170_01750"
                     /pseudo
     CDS             complement(6513..6829)
                     /locus_tag="VE170_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
     gene            complement(6857..8071)
                     /locus_tag="VE170_01755"
     CDS             complement(6857..8071)
                     /locus_tag="VE170_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14181.1"
                     /translation="MNPSSKDNSWRYITLAAAAMLMITMGARQAQGLFVFPISGSTGV
                     SIVAISFAMAVGQFMWGVAQPIAGACADRFGFTPVLIGGVLLLAVGTALTPFFTSSFG
                     LVVTIGILSAIGSGAGSFSVMIGALAQRLAPRERATASGLITGGGSFGQFLFAPITQK
                     LISTWGWMGAMWSLGAIALLALPLVKTVAGTERHVGAFAERDGGMKATLRTAFGNSSY
                     LLLHGSFLTCGFHIAFLVTHLPGEVDLCGLPASVASWTLAIIGLANIAGSFTAGWMLN
                     HFRGKYILFGMYGSRTILILIYLAAPKTEWTFYLLAVGLGFSWLGTAPPTAGVVGKLF
                     GTRYLATLYGMTMLSHQTGAFFGAWLGGLAFAHYGNYLSMWYADALFAAVAALLNLPI
                     REPRIERTAVPA"
     gene            complement(8227..9285)
                     /locus_tag="VE170_01760"
     CDS             complement(8227..9285)
                     /locus_tag="VE170_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HYJ14182.1"
                     /translation="MLSYDVVEWGKPLERIERITPMPQGTEVLVKLKYCGVCHSDVHI
                     RDGYFDLGGGRRLHMSERGMVPPVTLGHEPYGTVIAAGADAGDVAIGEDRLIFPWIGC
                     GACVRCREGLDNYCMAPNMIGIQRPGGYADHLIVPHRRYLIDASGIDPLWAATLSCSG
                     LSTYSAVAKLKPIPRNEWVAVVGAGGLGLSAIGMLRALGHERIVSIDIDDGKLSAAAA
                     AGAAATLHSRRSDAPENLKDITQGALYGAVDFVGSAETAELALDCLRKGGRLILVGLF
                     GGEIKLSIARTILRAVTVQGSHLGKVEELNAVVALARKGQIKPIPIEPRPLSEVSRTL
                     DELKLGGVVGRVVAEVSW"
     gene            complement(9383..>9993)
                     /locus_tag="VE170_01765"
     CDS             complement(9383..>9993)
                     /locus_tag="VE170_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYJ14183.1"
                     /translation="RGRPAGFRTHALVCLASALLMLVTVYQWDWLGAVPQETIRTDPT
                     RMAQGIMTGIGFLGAGTIMRDGLSVRGLTTAASIWITAAIGILIGIGFYVPAVVATFL
                     TLGTLSVFRWVESRIPTQLYSQFVVRFARAKAMPEAQMRQLLTDYGFTVANLNYRLDV
                     EADFIEYQMIIRTIRADNMSKLTQALNKIDAVKEYRVSPTGD"
BASE COUNT         2213 a         3040 c         2827 g         1913 t
ORIGIN      
        1 ctgctgcgca ggtatttcat catgattttg atcgtcggcg cgcgctgggt cttgccgtag
       61 tgaacgccct cgacgaagcc cctgatgaac ttgcgcaccg tgttttcgtt gcgtttgatg
      121 tacgaacggg tggtgatcac ggaggacgtg gggaaatgaa ctcccatctt ggcgatgtcg
      181 gcgagctcgc gaaaaccttc gcggcggccg aggatcgcca gctcggcatt gatcaccgcg
      241 gcgtcgagca cgccggcgcg cagtgcgttg taaacatcgg tgacgccgcc catctggagc
      301 acggcaaaat cgcgctggcg cttgatcggc cactgctctt cggcgtagcg cagaccgaag
      361 tcggtggccg agccgaagcg cgacacaccg atcttcttgc cttttagatc ctcgacgcgg
      421 cgaatctccg ggcgtgccat gacgacgaac gtcatgacgt tcatcgagct ggcgatgacg
      481 acgagatcgc cgccggcaag attggagtta atcaccgcgc cgccggtaaa gacaccgatc
      541 ggcacttcac ccgactgcac cacttgcgcc gcgcgcccgc cgccggcgac atagagtagt
      601 tcgacatcga ggccgtgctt gcggaagatg ccggcttcgt agggaaccca gaacgacgct
      661 tgcgatccgc cgatggcgct gtagaccaag cgcagttttt ccaaaggctg cgccgccacg
      721 ggagcaatga gcgaaagaga accgataaca atcgcaacga cgaacctgaa caggtggagc
      781 atgacagttc cttttctatg acttgcaaaa cggcccagca tcaaccggca acaaccagat
      841 cgaattcggc gtaaatcaac ggtaccaata tggcatgaag ttgccggtca aagtagcatg
      901 agcccagagg tcccaaccgg cgtcaagcgc ctagccaacc gccaccatga atgcagaatg
      961 gcgccaaccg acaaagcgac cacgctgaac aacagcgctc ctgcgcattc aacccagaat
     1021 gaccgccaac cgccgcgcaa cagcaaccct cccggatcga tcagtccgcg gggattagag
     1081 cgagaagccg attgtgaaga gtgcgccgca accgacccag gtcacgccgt tcacgaggta
     1141 gtcttcgcca gcgcccgcat caggacttgt tccagagctt catcggcgag tgaagctaag
     1201 cgatgatccg catgatccag caccaagcga caggtcaccg gattgggcac ggcaacgcaa
     1261 aagatgcccg ccgcccgcgc tgcggtgacg ccgttgggcg aatcctcgaa cgccaccgct
     1321 tcatgtggag cgatgccgag cgtgtcgagc accgcacggt agggagccgg atcaggctta
     1381 tgcgttgtgg tgtcctcgaa acacttggtg gtgtcgaagt agcgaagtag gtcgaggcgc
     1441 tgcaaatgac cgcgcacatg gttcgccgac gagctcgacg caatgccgag cttcaatccc
     1501 aggtcgccgg cgccgcgtaa gtattcttgc accccgggca acaccggttg ctgctcggtg
     1561 agtgcaatca cccgcgcccg ccgattaaga cgcaatgtgg cgatgtccgc tgtgggatcg
     1621 atatgttcat taaaataacc gtagagatcg aaatggttgc tcggccggcc gaccagatcg
     1681 gcccagacct ccaaatcaag ctcccgccca tgagcaatga agacttcctg ccaggccaaa
     1741 agctccggac tttcactatc gatgatgatg ccgtcgaaat cgaagatcaa cgctttaatc
     1801 ataggtgaga taaatcaaca aactctgctc aatcgcgaat tgccgctgca catcggcgat
     1861 ggtgcctctt ctcgttggtg ctcgtcaaca tcactgtgcc aaatgcaaac aatgctgcca
     1921 tccaaccttt gcggcaactc atggctcggc cctcccgcca catgataaat atttaaccat
     1981 caattcatta ctctaccgtc attgccgtcc gccgctttct tctcggcaac cttctcgacc
     2041 aggtcgcgaa agctggtgtt cgaatcgcac cggacgctaa aggcgacaac gaacaatgtg
     2101 atcaaatcct tggcatggat ccggtcatcg gcccaatcgg cgatttgagc gagagtatga
     2161 tccggtgtca actgcttgat cttcatgccg acgacttccg ccaggccggt ggcgagttgc
     2221 tgcgccacgg ctctatgtgc agccgggaaa tcgaggcgaa tgaatgccgt ttcgagggcc
     2281 cagtcagtgc ctttggcgcg gcggcgcact accactcggc ggcgcaagct gacaagtatt
     2341 aagattgcgc taaggactag aaaaaccgca gagagcatta tgacctgttt agcgggaact
     2401 ttagcgcgct ctcttcagcg ctgcaaaagc attgtagttg aactctggcg gctttacgat
     2461 gacgaccgct ctttaatcat ttgcgccatc gccaaactat ccgcgtcgac gctatccccc
     2521 agagccgcag cgacgccggc gcgatcctgg gctgggcgat tttggctgac cttccatttg
     2581 cccgccaacc ttgtaaccgc gatctcgatg ccgacgatgg cgcccagctg tttgtcgata
     2641 aaatccggcg gcgcgtcgct cacgtgccag ggggccgcgc tggcgctttc gaagcgcgcc
     2701 gtcaggcggg tcaccaagcc gcgcagccag gcgcgatctt caataattcg caacgcacca
     2761 gaggcatgga cgacggcata attgtaggtc ggcacgactt cgccggtctg tttcttggtc
     2821 tcataccaag aaggcgaaat gtaagcctgc gccccgtgaa aaatcaccaa cgcttcggtt
     2881 tcgcgggaat ggtcgcgcca gaccgggttg gcccgcgcca cgtgacagcg caaggtgccg
     2941 ttgggcgccg gcgcggcgtc gacttcgata ggcagatgat tggcgctgat tccgtcggcc
     3001 cccaacgtca ccaaggtcgc cagtggatgc tcgcgcatga gctggtgcaa gacttctatg
     3061 cgcttctctt caaagtgacc gggtagatac attgggatct ttccaactcg ctcgacttcg
     3121 ggcgtcgcgg cgccaagctt gagcgtgtac gaatatcccc ggcaatgatt ccactgtcaa
     3181 gcagcgcttc actccgtgct tgcattgctc gctgcacgct ttatatttgc tgcgcgagct
     3241 accttgtgaa cggtgcggag gaatcaatga cgatatcacg taaaccaaca atctgggccg
     3301 gtgtcttggt cacgctcacg ctattatggc cgtcaccgcg gctgcatgcc caggaatcct
     3361 tcttcaaggg caaacagatc cgcgtcatcg ttggcttgag caccggcggc ggctacgacc
     3421 gcgcggcgcg gctgatctcc cgatacatgg gcaagtacct gcccggcaat cctgatttcg
     3481 cggtgcaaaa catgccgggc gccggttcgg taaccgccgc caactatctc tggagcgtgg
     3541 caaaaccgga cggcctgacc ttgctggcac cgcataataa tttttatctc gcccagcttt
     3601 ccggccagaa agaagtgaag ttcgacctga ccaaatttca atggatcggc tccctggaga
     3661 acgacgacat gatcatgttt gcccgcgccg acgcgccgtt cagatccgtc gctgacatca
     3721 ttcgctcgaa ggagccgccc aaatgcggct ccaccggcgt cggcagttcg gattacgtca
     3781 tgtcgaagat cctcgaagag accgtcggca tgaaggtggg ccatgtcacc ggctatcccg
     3841 gcagcagcga gatcgccctg gccatggagc gcggcgaggt cgcctgcatg ggattgacca
     3901 tcgccacata ctacggccgc gagccatttt tgagctggct caagaccaag ttcgtccgct
     3961 tcctcgccca gagcggccac aagcgttcgc cgcgcgtagc cgatgcgccg acgatctacg
     4021 aattgatgga cgagtacaaa accgcgccga ccaagcggcg cgtcgccgag gcgatgctcc
     4081 aaggcggcga atgggcaagg ccgttgatcg cgccgccggc aaccccgccc gaccgggtca
     4141 aaatcttgcg cgacggctat gaaagagctt cgaaagatcc ggaattactc gcggaggcga
     4201 aaaagctttc catcgaaatc gcgcccctgc gcggcgagca attgcagaaa actgccagtg
     4261 acatcatcaa gcagccggcg gaagtaaccg aacagatcaa gaaactgttc gttcaataat
     4321 ctagcgagga gctccaggcc agactgtcca tgggacggtc tctggcggcg cactaacgag
     4381 cacccggcag tgaaagaact tagcctttac cagcggccgc ggcccggccc ataggcgcgt
     4441 ttggcgccgg cccgggcgcc gaaagtcggt ttagggttgg gatcgagcaa acgggtgaat
     4501 gcgtaattga aattttccaa ttttaatcgg ggaatttttt ggccgatgaa atgctcgatg
     4561 gcggcgacct cctggagatc ctctgcggtc atcaaggtaa aggcatcgcc tacgcttagc
     4621 gcccggccgg tgcggccgat gcgatggacg taatcttccg gatgatgcgg cacatcgtaa
     4681 ttgatcacat ggctgatctg ctcgacgtcg ataccgcggg cggcgatgtc ggtggcgacc
     4741 atgacttcgt agcggccgtt gcggaatcca ttgagcgcct gctcgcgctc gcgctgggtg
     4801 cgattcgaat gcagcacggc caccgcatgg cccttctccg tgagccggcg cgccacccgg
     4861 tcggcgccat tcttggtgcg gcagaagatc aacacctgat cgtattcggt tcgctccaat
     4921 aactctgtta gcaattcttg tttctgcgcg acatcgacgg gataaagcgc gtgggtgatg
     4981 gtttcggcgg gcgaacggcg ctggccgatc tgaattgttt cggggttttt catcgcccag
     5041 cgcaccagcg cgtcgatctc aggcgggatc gtcgccgaga acaacatggt ctgccgggct
     5101 ttgggcacgc ggtcgacgat tttgcgcacg tccggcataa agcccatatc gagcattcgg
     5161 tcggcctcgt ccagcactaa aaactcgatg tcgttcaagc gcagcatctg gcgttgcatt
     5221 tgatcgagca accggcccgg cgtcgcgacc acgacatcgg taccgcggcg cagtgctaga
     5281 tcttgcttgc cgtagcccgt gccgccgaac aagacgacgg tgcgcaggtc ggtaaagcgc
     5341 gagaaatcat gaaaggcggt ctcgacctgc gccgccaact ctcgcgtcgg ctcgagtatc
     5401 aacgcgcggg ttttaccatg ctggccgaga cgagaaatga tcggcaaggc aaacgcggcg
     5461 gtcttccccg tgccggtctg tgccgaaccg ataatatccc ggccttcgag cgccagcgga
     5521 atggcgcgca gttgaatcgg tgttgggtcg gtgtaaccgg ccgccttgac cccttcgagt
     5581 accttgtctg acaaaccgag cttcgcaaat agcataccgt tactataggc acgaccacgc
     5641 gtgcttctca atggcagggt tagcgggcgg taaataaccg cgatgccggc gcgcgggtcg
     5701 tcagttcact gcgcggcgat ctaacgcgct caccgagcac agcacggatg aaatcactct
     5761 ttgccttgga atactctgtg ctcgcggtga taccgctgga gagaatcgac aatttctcaa
     5821 cttggtaggc ttgcactagc tcggcatcgg cacgcaagca atcgcgaaac ttgatgaggt
     5881 tttgcgcctc agggtcgccg ctgcggatca catgagcatg aacccggtag gccttgccgc
     5941 gataggtcgt caccccgaca cgcatcggcc gcgattcggg aaatggttca gggccgctct
     6001 gcggttggaa gcccagctga tcgagggcat ccctggcgat gaaaaacgcg cctgccggat
     6061 aagttaccaa caaatcgatg atgcccttgc cgccgcagcc gggtaccgcc gtgctgccaa
     6121 tgtgatcgac ataaagggtc gaccgataat cgagaatcgc atcgatgagc cgcgcggcgg
     6181 ctcgtggcgc ttcaggatcg tagggaaaat agcgcgccag tttggccgcg tagcgctcaa
     6241 cttttaccgc cggacgatcg cccaagtatc ttgctgccaa caatttcgca tcctcgcttt
     6301 caagacttct gccaactaga cgagcaagcg gaatgccagc aggtgcaacc tatgtaacta
     6361 aaggataata gcgacgaatg gggtcgcggc cgctacgacg gaacgcggca ggcagcaacc
     6421 gcagattaca ccagcggcaa ccacggttgc agggattcat gagcgggtca aaacaatgca
     6481 accgcgcctg cgaatgatcc tcaacgggaa atctacgagc cgatgaaaga gcagcaataa
     6541 tcggtcaacg ttttgacccc catgcgaaat ttaccatttg ccgagacttt gaaagtctct
     6601 cccgccttga tcgctttgac gccagcgcca ccggcgagcg caacatccaa ctcgccgctt
     6661 attatctcca tgagctcttt gtcaccggtg ttgaattcgt attcacccgg ctgcatgatc
     6721 cccaaggttt ttttccaacc atcggcaaag accaccgtgc ggctgacgac tttacctcga
     6781 agtagacatt ggcttttttc atgactgtga cattggtgaa ttccgaaatc gggaaatcct
     6841 cctgaccgaa ttgaatttat gccggcaccg ccgtgcgttc gatgcgcggt tcgcggatcg
     6901 gtagattcaa aagcgccgcg accgccgcga acaaagcatc ggcataccac atcgacaaat
     6961 agttgccgta gtgggcgaac gcgagtccgc ccagccaagc gccgaaaaac gcgccggtct
     7021 gatgcgatag catagtcatg ccatagagtg tcgccaggta gcgggtgccg aataacttcc
     7081 ccaccacgcc ggcggtgggc ggcgccgtgc cgagccagga gaagcccaag ccaaccgcca
     7141 agagataaaa cgtccactcg gttttaggcg cggcgagata gattaagatc aagatcgtgc
     7201 gcgagccgta catgccgaac aaaatatact tgccgcggaa atgattgagc atccaaccgg
     7261 cggtaaagct gccggcaatg ttggcaaggc cgatgatcgc caaggtccaa ctcgccaccg
     7321 aggcgggcaa accgcaaagg tctacttctc ccggcagatg cgtgaccaga aaagcgatgt
     7381 gaaaaccgca ggtgagaaaa ctaccgtgga gcaacaggta actggagttg ccaaaagctg
     7441 tccggagtgt tgccttcatg ccgccatcgc gctcggcgaa cgcaccgaca tggcgctctg
     7501 tcccggcaac ggttttgacc agaggcaatg ccaacagcgc aatcgcgccg agggaccaca
     7561 tagcgcccat ccagccccag gtcgagatta atttctgggt aatcggcgca aagagaaact
     7621 ggccgaacga tccgccgccg gtgatcagcc ccgacgctgt tgctcgctct cttggcgcca
     7681 agcgctgcgc caacgcgccg atcatgaccg aaaaactccc cgccccggag cctatggcag
     7741 aaaggattcc aatcgtaacc accaggccga aactgctggt aaaaaacggc gtcagcgccg
     7801 tacccacggc aagcagaaga actccgccaa tcaacaccgg cgtgaaaccg aagcgatccg
     7861 cgcacgctcc cgctatcggc tgcgccaccc cccacataaa ctgccccacc gccatggcga
     7921 aactgatcgc aacgatactc acgcccgtcg atccactgat cggaaagaca aacagaccct
     7981 gcgcctggcg cgcccccatg gtaatcatca gcatcgccgc cgcggcgagg gtgatgtagc
     8041 gccaggaatt gtctttgcta gaaggattca ttatggcgaa ccaatgcagt cattctacga
     8101 tgcacaatcc gcggatcggc cacgcctgcc attgatcgcc gatctttccc agccgatgga
     8161 ttgtaacgta aagtatgaca tctgttttgt catgccggcg cgggcgtatc cagtctgccc
     8221 ccgccgctac caggaaactt ctgccacgac tctgccgacg acgccaccaa gcttcaactc
     8281 gtccaatgtg cggctcactt cagacaatgg ccggggttcg atcgggatcg gttttatttg
     8341 tcccttccgc gccagcgcaa ccaccgcgtt caactcttcg accttgccaa gatgggagcc
     8401 ttgtaccgta acagcgcgca gtatggtacg agcgatggat agtttgatct caccgccaaa
     8461 caaaccgacg agaatcaacc tgccgccttt gcgcaagcag tccaacgcca actccgcggt
     8521 ctcggcgctg ccgacaaaat ccaccgcgcc gtacaaagcg ccttgcgtga tatctttgag
     8581 attttccggc gcgtcactcc tccggctgtg gagcgtcgca gctgcgccgg ctgccgctgc
     8641 cgcggacagt ttgccatcgt cgatatcgat tgaaacgatg cgctcatggc cgagcgcgcg
     8701 cagcatgccg atggccgaca aacctagtcc acccgcaccg acgacagcga cccattcgtt
     8761 gcgaggaatc ggcttaagtt tcgcaaccgc ggaataagtt gacagccccg agcacgacaa
     8821 cgtcgccgcc cacagcggat cgatgccgct ggcatcgatc aaatagcgcc gatgcggcac
     8881 gattaaatga tcggcgtaac cgccgggtcg ctggatgccg atcatgttcg gcgccatgca
     8941 gtaattatcc agcccctcgc ggcagcgcac acaggcgcca caaccgatcc aaggaaaaat
     9001 caagcggtct tcaccgatgg caacgtcgcc ggcgtccgct ccagcagcga tgactgttcc
     9061 gtacggctca tggcccaagg ttaccggcgg caccatgccg cgctcgctca tgtgcaagcg
     9121 cctgccgccg ccgagatcga aataaccgtc gcggatatgc acgtcgctgt gacacacgcc
     9181 gcagtatttc agcttgacga ggacttccgt accctgtggc atcggcgtga tgcgctcgat
     9241 tcgttcgagc ggcttgcccc attcgacaac atcgtagctc agcatatccg gtactcgaac
     9301 tggaccgcag ttgatatacg acccgccgcc ttggcccgaa gcggcgcttg gcattttcaa
     9361 ttatcgattc ggcgccgacg cttcaatcgc cggtgggcga gacgcgatat tctttcaccg
     9421 cgtcaatctt gttgagcgct tgcgtcagct tactcatgtt gtccgctcgg atggtgcgga
     9481 taatcatctg atactctatg aaatcggctt cgacgtccag ccgatagtta agattagcga
     9541 ccgtaaaacc gtaatcggtt aacaactggc gcatttgcgc ctccggcatc gctttagcgc
     9601 gcgcgaagcg cacaacgaat tgagaataga gctgggttgg aatccggctc tcgacccatc
     9661 taaacaccga cagcgtgccc agcgtcaaaa aggtcgcgac cacagcgggc acataaaacc
     9721 cgatgccgat tagaataccg atggcggcgg tgatccagat cgatgccgcg gtggtgagac
     9781 cgcgcaccga aagcccgtct ctcatgatcg tacctgcgcc gagaaagccg ataccggtca
     9841 tgatgccttg cgccatgcgc gtcggatcgg tccggatggt ttcctgcggc accgcgccga
     9901 gccaatccca ttgatagacg gtgaccaaca tcaggagtgc tgaggccaga caaacgagtg
     9961 cgtgcgtacg aaatcccgcg ggccggccgc gga
//
LOCUS       DASQKS010000068         5601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5496993, whole genome shotgun sequence.
ACCESSION   DASQKS010000068 DASQKS010000000
VERSION     DASQKS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5496993"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VE170_01770"
     CDS             complement(<1..502)
                     /locus_tag="VE170_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14184.1"
                     /translation="MKRSTERILTTHVGSLPRPVDLRAMWAKQSSAAEDEAALKIRLR
                     SAIGDVVLAQQSAGIDIPNDGEFGKPMRAATDRGAWGNYIFDRLSGFTATSPQTLAPD
                     TAAPGAPMRVVGVRWEQREFAEFYADTGLGAPSTAAMRPTCTGPLGYTAQIFLREQLA
                     TLKKAID"
     gene            complement(1312..1908)
                     /locus_tag="VE170_01775"
     CDS             complement(1312..1908)
                     /locus_tag="VE170_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HYJ14185.1"
                     /translation="MATTTERPLADALTAIKSRRSVKEYLPTEIPREWIEELLDAAHW
                     APNHKLTHPWRFHVFSGEGRERLVAARQASVRWAAEKKGEQASEEALKFARDKCFSSP
                     VLIIVSMVGDDKKITDQENYAACWCAIENLLVAANARGLGSYPSTGDWIDQNFVGPIL
                     GLSEKERPVACIFLGYSEQATMAKRLPVDRHTTWYTDA"
     gene            complement(2124..3107)
                     /locus_tag="VE170_01780"
     CDS             complement(2124..3107)
                     /locus_tag="VE170_01780"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14186.1"
                     /translation="MMSRIAFAAFPKFFILTLLFSQTWAQPVKLTVSYTGVGPVNLPV
                     VLAKEAGIFARNGLDVTVVRAQAAVSTMALVSGEVGIIQAAAPAVLQSNLGGSGAVYI
                     AGGYTGLDYWLIGHPSIKSTEQLKGAIIGAAGLSGGSYTATQLAVRKLGLNPAKDVTI
                     IAIGGTPERLAALRAGRVQATSLNPPTIFAAEKEGFKILADVSALPFQNVAPVTTRRF
                     IKENPDIVRRYLKSQVEAVHMMKRDRKTGLRVFTQLMAGFKDTAVLEKSYDVSVTDDK
                     LPRSQYPSLEGIKAVLDTLGDRGKDARPTDFVELRFIKELDDSGFIQALYK"
     gene            3645..4448
                     /locus_tag="VE170_01785"
     CDS             3645..4448
                     /locus_tag="VE170_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14187.1"
                     /translation="MALRINDAEFYAIDAHSHLGRRKTPLGHGVASFLGEDLVRNLDE
                     AGLDCAVAFPLGASYTDYSEANQIMAEEMAKYPKRIISFCRINPNFGPEATAKALDHC
                     LGTLKLRGIKLHPEIEFFDPNEAELMEPIYDAARRYHVPIIFHTGMSSKASPGVIAEL
                     AAQNKDVPVILGHMGVSEYVKLAVAVARQNENIFLETSVVGWMPLLLEAFRGVGTSKI
                     LFGSDHPYNPLAMEVEKIAKHVTKAAKLKQDDLKKVFADNFLSILQHDR"
     gene            4541..5545
                     /locus_tag="VE170_01790"
     CDS             4541..5545
                     /locus_tag="VE170_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14188.1"
                     /translation="MRPSEKSIRFTLLSVLVGVIFVLATGSSRARAAEMPSVRANYAA
                     FSGAFAPLWIAADRNLFTKYGLNVDLRYIAPATATQALIGKNLDIINPGGEIVEAGLN
                     SEPVVYIAGIMNRAVMSIYAKPEIASLADLKGKVLAVTVPGATTDFAARVLLQQARLT
                     PGKDVKLIYLKGMVEILTGINQGNADAGIFTSPTTLKAQHAGLKELVNVTEQNIPMIH
                     AALASTKEYVKTRRDDARRFLQAYLEGIKISRTDPEYTKQIIGKYTKTTDVADLENSY
                     QTFLPAWERLPLVPAAAVQTMLNFASHPGAKTAKPEAFIDNSILTEIDKSGFIDKLYK
                     "
BASE COUNT         1241 a         1571 c         1541 g         1248 t
ORIGIN      
        1 catcgattgc cttctttaag gtcgccagtt gctcgcgcaa aaaaatttgc gctgtgtagc
       61 cgaggggccc ggtgcaggtt ggccgcatgg cggcggtact cggtgcgccc agtccggtgt
      121 cggcgtagaa ttcagcgaac tcgcgctgtt cccagcgcac gccgacgacg cgcatcggcg
      181 cgccaggcgc ggcggtatcg ggcgcgaggg tttgcggcga agttgccgtg aagccggaga
      241 ggcggtcgaa gatatagttg ccccaagcgc ctcgatcggt ggccgcgcgc atgggcttgc
      301 cgaactcgcc gtcgttggga atatcgatgc cggcgctttg ctgcgccagc acgacgtcgc
      361 cgatggcgga gcgcagccgt attttcaggg cggcttcgtc ctcagctgcg ctggactgct
      421 ttgcccacat ggcgcgcagg tcaaccggcc gcggcagact gccgacgtga gtagtgagaa
      481 ttcgttccgt gcttcgtttc ataggcgctg cctcgcgttg gttttgctgc gaactggcga
      541 atgatgtcgg ccggagacga aacggttatg tccaagaacc caaccctggc gcaatccgga
      601 ccgcttttcg aaatctacct atcctattga cgcaaagttg cttgacggtc aacaagcggc
      661 agcgccaatt acaatgagca cagcgtgtat tacatccctg aaataacgat acgagcgcaa
      721 tccggtcaag gcccggtcgg aactaacttc acggtgaaga tataggaaga cacggcggta
      781 tccgagctgg tgttggtatt cgggagcatg ctttggacgc ccctgacaat gtgagccggt
      841 gaacggaatc actggaaact cggcgtccat cagaaattgg gcgaacacgc cgtcgcggtc
      901 gcgcttgatt ggcgtgactt gattgatgaa cggcaattgc gcgtcggtct ggaatatgtc
      961 cgccccaagg tcgcccagaa tgccaatccg ccgagcccga ccaacactcg tctcataagt
     1021 gtgtaccctt ttctctccgt gcaggcgcga gccggctgcc cggcgatggc ttcccagctc
     1081 cttcgtagtg tcctgtcgac ggttcttact tcaggcgaga gattgaggga tcaggtccgc
     1141 cattcagata tcgcgttcgg ctctttatag cagagagact cgggggcggg actagaatct
     1201 tgaagttaca aactcaacac aatcacccaa ttgcccgaaa gagggtcgag gacacgaccc
     1261 tctattcggt gcaattcatg aaagctgcgg ctgcgtttgg ttcgagcaat ttcaagcgtc
     1321 ggtgtaccac gtagtgtggc gatccacggg caggcgtttg gccatggtcg cctgctcgga
     1381 gtaccccaag aaaatgcacg ccaccggccg ctctttttct gaaagtccga ggatcgggcc
     1441 aacgaagttc tggtcgatcc agtcgcccgt gctgggatag ctgcccaggc cgcgcgcgtt
     1501 ggcggcgacc agcagattct caatggcgca ccagcaggcc gcgtaattct cctgatcggt
     1561 gattttttta tcatcgccga ccatcgaaac gataatcagg actggggacg aaaaacattt
     1621 atcccgggcg aatttgagag cctcttcgct agcctgttcg ccctttttct ctgccgccca
     1681 acgtaccgac gcttgacggg ctgcgaccaa tcgctcgcgc ccctcgccgg agaacacgtg
     1741 gaaacgccag ggatgggtca atttgtggtt tggcgcccag tgggcggcgt cgagcagctc
     1801 ctcgatccat tcccgaggaa tctcggtggg aaggtactcc ttcaccgagc ggcgcgattt
     1861 gattgcggtg agcgcgtcag cgagaggacg ctccgttgtg gttgccatga ttctacctcc
     1921 tggttttgtg ccgattctat gtatagggaa cttaaaccat tttcctggcc cgagcagtat
     1981 cgctggaccg ggaagttgtc caattagggt ctagcagttt tataacctga catcttgcaa
     2041 atcaagttgc agcgtccggt cttccagctt gccgttattc tgcatatcaa cgcgccgtac
     2101 cagaaatgaa aaatgccctc gtactattta tacaacgctt ggatgaagcc gctgtcgtcg
     2161 agttccttga tgaaccgcaa ctcgacaaag tccgttgggc gcgcgtcttt gccgcggtcg
     2221 ccgagggtgt ccaaaaccgc cttgatgccc tccaatgaag gatactggct gcgcggcaac
     2281 ttgtcgtcgg taaccgatac atcgtagctt ttctcgagca cagcggtgtc cttaaagccg
     2341 gccatgagct gcgtgaaaac tctcaagccg gtttttctgt cacgtttcat catatgcacc
     2401 gcttctactt gtgacttcag gtagcggcgc acgatatcgg gattttcttt gatgaatctt
     2461 ctagtggtca ccggcgccac gttttgaaat ggcaatgcgc tcacgtccgc taaaattttg
     2521 aagccttctt tttccgcggc aaagatcgtc ggcgggttga gcgacgtcgc ttgcactcgc
     2581 ccggcgcgca aagccgccaa gcgctccggc gttccgccga ttgcaataat cgtaacgtct
     2641 ttcgcgggat tgagcccgag ctttctgacg gccaattggg tcgccgtgta cgagccgcct
     2701 gatagacccg ccgccccaat aatcgcgcct ttgagctgtt cggttgactt aatgctcgga
     2761 tgaccgatta accagtaatc gaggccggtg tagccgccgg cgatgtaaac cgcgcccgac
     2821 ccgccaagat tgctctgtag gacggccggc gccgccgctt gaataatgcc cacttcaccg
     2881 gacacgagcg ccatcgtcga taccgcggct tgagcgcgaa caactgtgac gtcgaggccg
     2941 ttcctggcaa atatcccggc ttccttcgct aaaacaaccg gcagattgac cggaccgaca
     3001 ccggtatagc tcacggtcag tttcaccggc tgtgcccagg tttgcgaaaa caacagcgta
     3061 agaatgaaga atttgggaaa cgccgcgaac gcgatcctgg acatcattct caaccccctg
     3121 cgcaacttgc aattcatcat ccgttgccgt ccgtccttct aacactgtct ttggggaagt
     3181 cttgtgtggg agcgcgcgcc taggtatcga gtgatttgcg ttctcccgtc ttggtcggaa
     3241 ggccagtcac cggtacttaa gcctataccc tttgctcgaa cttagatcag agaagagcgt
     3301 ctgcgggacc gaccatcgaa tcgtcgttgt tatctcacaa tcgagtctta gcaagtaaat
     3361 cgaccggcgc atcgcgctgc aaaatgatcc gtacttcccg ggcttgaagg tcgatttggg
     3421 aagcgtaccg gcagtctcac tcgtttttct catgcgcgta aagtaggcaa agccagggcg
     3481 ttcgtcaatg tgaaaatggt ctgtgcacct tttccgatag ttgccatgct ttacattgtt
     3541 gcccagagtg gcccgaacaa attcccgatt acttgagcga cgctattgcg gatgtaaatc
     3601 gaacgaaata aacattggct caaaccaatc ccaggagaac atacatggcc ttacgcatca
     3661 atgacgcgga attctacgcg atcgacgctc actctcattt gggacgccgc aagacgccgc
     3721 taggacatgg cgtagcgagt ttccttggcg aagacttggt gcgcaatttg gacgaagccg
     3781 gcttagactg cgccgtggca tttcccttgg gagcttccta caccgactat tcggaagcga
     3841 accagatcat ggccgaggaa atggccaagt atcccaaacg gatcatcagc ttttgccgta
     3901 tcaatccgaa cttcgggccc gaagccacgg cgaaagctct cgatcattgt ctcggcacct
     3961 tgaaactacg gggcatcaaa cttcatccgg agatcgagtt cttcgatccc aatgaggccg
     4021 agctcatgga gccgatttac gacgcggcgc gccgctatca tgtgccgatt atttttcaca
     4081 ccgggatgtc gagcaaggca tccccgggcg tgatcgccga gcttgcggct cagaataaag
     4141 acgtgccggt tatcctcggc cacatgggcg tatcggaata tgtcaaactt gcggtggccg
     4201 tcgcccggca gaacgaaaat atattcttgg aaacttcggt ggtcggttgg atgccgctgc
     4261 tgcttgaagc gtttcgcggc gtcggcacga gcaagattct cttcggctcc gaccacccct
     4321 ataatcccct ggccatggaa gtggagaaaa tcgccaagca tgtgacgaaa gccgccaagc
     4381 tcaaacagga cgacttgaag aaggtgtttg ccgataattt tttatcgatt ttgcagcatg
     4441 accgctaggt cgggtatgcg aattcttgag tcagcgtgaa gagcagcttt cgctacccgc
     4501 cattacacgg cacttaaaaa cggcaaggat aggagtttag atgagaccct ctgagaaatc
     4561 gattcgattc actttattgt cggttttagt aggcgtaatt ttcgtcctcg ccaccggctc
     4621 atcccgagca cgcgcggccg agatgccctc ggtacgggcg aactatgcgg cctttagcgg
     4681 cgccttcgcg ccactgtgga tcgcggcgga tagaaatctg ttcaccaagt acggtttgaa
     4741 cgtcgacctc cgctacattg cccctgccac ggcgactcag gcgctcatcg gcaagaacct
     4801 cgacatcata aatcccggcg gcgagatcgt cgaagccgga ctgaatagcg aaccggtcgt
     4861 ctacatagcc ggcattatga atcgcgcggt gatgtcaatt tatgcgaaac cggaaatcgc
     4921 ttcactagcc gacctcaagg gcaaggtgct ggccgtgact gtgccggggg ccaccaccga
     4981 ctttgccgcg cgggttttgc tccagcaggc gcgcttgacg ccgggtaaag acgtcaaact
     5041 tatctattta aaaggcatgg tggaaatctt gaccggcatc aaccagggca acgcggatgc
     5101 aggtatcttc acatcgccga cgactttgaa agcccaacac gccggattga aggaattggt
     5161 caacgtcacc gagcagaata ttccgatgat tcacgccgct ctggccagca ccaaagaata
     5221 tgtcaaaaca cgccgcgacg atgcgcggcg gtttttacaa gcttatctgg aaggcatcaa
     5281 aatctcccgc accgatcccg aatacaccaa acaaatcatc ggcaagtata ccaagaccac
     5341 cgatgtcgcc gacttagaga attcctatca gacattctta ccggcatggg aacgtttacc
     5401 gttagtcccg gccgcggccg tgcagacgat gcttaatttt gccagccatc ccggcgcaaa
     5461 aacagcaaag ccagaagcgt tcatcgacaa ttctatcctc accgaaatcg acaagtcggg
     5521 attcatcgat aaactgtaca aataagacaa aatctccgtg tgagattggc ggtctgaaaa
     5581 aggagaccta agggaattgg g
//
LOCUS       DASQKS010000069         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5076751, whole genome shotgun sequence.
ACCESSION   DASQKS010000069 DASQKS010000000
VERSION     DASQKS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5076751"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VE170_01795"
     CDS             <1..319
                     /locus_tag="VE170_01795"
                     /inference="COORDINATES: protein motif:HMM:NF021205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HYJ14189.1"
                     /translation="STTANLIVDTGAGQTVLSKRAARDLGLMTVDTQARLGIGGAVKV
                     DVGVVESVKVGAAELKNLPISIHDFSPDPRIEGLLGFDFLGRFQMSLDQNKQVIVLTP
                     KKD"
     gene            348..1340
                     /locus_tag="VE170_01800"
     CDS             348..1340
                     /locus_tag="VE170_01800"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14190.1"
                     /translation="MKRKQLTRVCRSFALIGSAILIFLTAGFVSAQERIKFPVGVGTK
                     TLGTGLIWLATKKGFFEEAGLDVQPVLLRGTPVAVQALVGESLFMTLGSADAMVSAAV
                     GGADLVSVAGVINGLTQAIVAGKKYKSFKELRGTTIGVQSLASGATTVLKRIFKKHGL
                     DYPADYKLLAVGGGNFNLAALSSGQVAAAFLVVPLVFTAEEQGMNVLGYYKDYYPNYQ
                     LTVAAVKRGWAEKNRALLVRFLRAGLRANRWLYANKEAASDFFAKEIQMSPELARKGW
                     EYYTANRIWHPNLELNLDGMKLALEILAEETKQVPQDAVKFIDGRYLQQALKDL"
     gene            1348..1719
                     /locus_tag="VE170_01805"
     CDS             1348..1719
                     /locus_tag="VE170_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYJ14191.1"
                     /translation="MKDDLKPGLTHTQTYETTERMRATQLTADVFSTPAMIGLMEGTC
                     VRLTAPYLDEGENSVGMHVDVHHLAPTKIGQRVTVTAEILEIDGNKIRYAVSAVNDAG
                     VKIGAGKHRRAVIRPKKLSDG"
     gene            1941..4010
                     /locus_tag="VE170_01810"
     CDS             1941..4010
                     /locus_tag="VE170_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYJ14192.1"
                     /translation="MSSSQVILPTAGQADEDPYLWLEDVGGERALTWVRAHNAASTGE
                     LEGVAGFEGLRRRLLAIYESNERIANVTKHGAYFYNFWRDEQQVRGVWRRTTLAEYRK
                     AEPAWEIVLDLDRLAASEKENWVWKGADILQPGHDRALLFISRGGADAAVMREFDLIK
                     KEFVVDGFVLPEAKSRVAWRHRDALYVGTDFGSGSLTKSGYPRIVKEWLRGTALQVAK
                     TVFEGQTDDVSVAASVVHDHGRIYEIISRGLTFFTGELLLRRGDDWIRIDKPADARVE
                     TYTDQILLRLRSEWRVDGKTYRAGSLLAADFAAYLRGEREFAVLFEPSHRKALASTSQ
                     TKNFLILNELDNVRSKVYALKRADGQWQRQQLATPAFGSVRVRGVDAHADDASDDYFM
                     SITDFLTPSSLVLGRVGAPTEEKLKSLPAFFRADGLAIDQHEAVSKDGTKIPYFQVSR
                     KDLPTDGKHPTLLYGYGGFNVSQLPFYSGTLGAAWLERGGVYVLANIRGGGEFGPNWH
                     YAARKENRQRAYDDFIAVAEDLIARKVTSPQHLGIQGGSNGGLLVGAALTQRPDLFKA
                     VVCQVPLLDMRRYSKLLAGASWIDEYGDPDKAEDWAYIAKYSPYHNVVAEKKYPRVLF
                     TTSTRDDRVHPGHARKMFAKMKAQGHDVLYYENIEGGHGGAADNRQLAYMSALTYAFL
                     VKELFGQ"
     gene            complement(4092..>4289)
                     /locus_tag="VE170_01815"
     CDS             complement(4092..>4289)
                     /locus_tag="VE170_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14193.1"
                     /translation="SPDTVVRQIKSIANQAGGDTFLGEFTFGELTQKQALNSLRLFTE
                     QVMPELRTFEVGALNFPRADA"
BASE COUNT          908 a         1180 c         1323 g          878 t
ORIGIN      
        1 ctcgaccacg gcgaatctga ttgtcgatac cggcgccggc cagacggtgc tatcgaagcg
       61 agccgcccgc gatctcggct tgatgacggt cgatactcaa gcgcgattag gtatcggcgg
      121 tgccgtgaaa gtcgacgtcg gcgtcgtcga atcggtgaag gtcggcgccg ctgaactgaa
      181 aaatctgccg ataagcattc atgatttttc gcccgatccc cggatcgaag gtctcctggg
      241 tttcgatttt ctcggccgct ttcaaatgtc gctggatcaa aacaagcaag taatcgtgtt
      301 aactccgaaa aaagactagc tcatggctaa gtgggtggtg gcccaaggtg aagcggaagc
      361 agttaacgcg ggtctgtcgg tctttcgccc tgatcggcag cgcgatttta atatttctca
      421 cggcgggatt tgtcagcgct caggagcgga tcaagttccc cgttggcgtc ggcaccaaaa
      481 ccctcggcac cgggctgatt tggctggcca ctaagaaagg cttttttgaa gaagccggcc
      541 tcgatgttca gccggtgcta ttgcgcggca cgccggtggc ggtgcaggcg ctggtcggcg
      601 aatcgctttt catgaccctg ggttccgccg acgcgatggt cagcgcggcg gtgggcggtg
      661 ccgatctcgt gagtgtcgcc ggtgtcatca atggtttgac ccaagccatt gtcgccggca
      721 agaaatataa gagttttaaa gaactgcgcg gcacgaccat tggcgtgcag tcgctggcat
      781 cgggcgcgac cacggtgctc aagcgtatct tcaaaaagca cggcctggat tatcccgccg
      841 attacaaact cttggcggtg ggcggtggca acttcaatct ggcggcgctc agttcaggtc
      901 aggtggccgc ggcatttctc gtcgttccat tggtgttcac cgccgaagag cagggcatga
      961 acgtgctcgg ctactataaa gactactatc ccaactatca gttgacggtg gcggcggtga
     1021 agcgcggctg ggcggagaaa aaccgcgcgc tgttggtgcg cttcctgcgc gctggcttgc
     1081 gtgccaaccg ttggctgtac gccaacaaag aggcggcgag cgatttcttc gccaaggaaa
     1141 tacaaatgtc accggagctg gcgcgcaagg gttgggagta ttatacggct aaccgaattt
     1201 ggcatccgaa cctcgagctt aacctcgacg gcatgaagct ggcattggaa atccttgccg
     1261 aagagactaa acaagtaccc caggacgccg tgaagtttat cgatggtcgt tatttacagc
     1321 aggcactgaa ggacttgtga gacggttatg aaagacgatc tcaaaccggg actgacacat
     1381 acacaaacct acgaaaccac cgagcgcatg cgcgctacgc aattgaccgc cgatgttttc
     1441 tcgacgccgg cgatgatcgg cctgatggaa ggaacttgcg tgcggctgac cgcgccctat
     1501 ttggacgaag gcgaaaatag cgtcggcatg catgtcgacg tgcaccactt ggcgccgacc
     1561 aagatcggcc aaagagtaac cgtcacggcg gaaatcttgg agatcgacgg caacaagatt
     1621 cgctatgcgg tgtccgcagt caacgatgcc ggcgtaaaaa tcggcgcggg caagcaccgg
     1681 cgcgcggtga ttcggccgaa gaaactatcc gacggctgat cggcgcgcca tggtgttgac
     1741 cgatgaaacg gcaatgaccg cgtgctgtgg taagatcaga cgccgttttt gaactccgcg
     1801 gaggacttcc agcgccatcc gcaggttacc gcttgctgcc catcgactag ctatcgttat
     1861 caattatgcg gagcgtggtg cgtcatgtcg gagttttgct cgttagcgtc gcgattctct
     1921 atgccctggc gaaaacaaca atgagttcat cccaagttat cttgccaacc gccggccaag
     1981 cggacgaaga tccatatctg tggctcgaag atgtcggcgg cgagcgcgcc ctaacctggg
     2041 tgcgcgcgca caacgcggcg agcacgggcg aactggaagg cgtcgcaggt ttcgagggcc
     2101 tgcgccggcg tttattggcg atctacgaat ccaacgagcg catcgccaat gttaccaagc
     2161 atggcgcata tttttacaac ttctggcgcg atgagcagca ggtgcgcggg gtttggcggc
     2221 gaacaacgtt ggcggagtat cgcaaggccg aacccgcctg ggagatcgtc ctcgatctcg
     2281 atcggctcgc ggcgtcagag aaagagaact gggtctggaa gggcgccgat atcttgcagc
     2341 ccggccatga ccgcgcgctg ctgtttattt cgcgcggcgg cgccgatgcc gcggtcatgc
     2401 gcgagttcga tctaatcaaa aaagagtttg tcgtcgatgg attcgttcta ccggaggcga
     2461 agagccgggt ggcttggcgc catcgcgatg cgctttacgt cggtaccgac ttcggctccg
     2521 gctcgctgac caaatccggt tatccgcgca tcgttaagga atggctgcgt ggtacggcgc
     2581 tgcaagtggc caagactgtt ttcgaagggc aaactgacga cgtcagcgtg gcggcatcgg
     2641 tggtgcatga tcacgggcgt atatacgaaa ttatttcccg tggcctgaca tttttcaccg
     2701 gtgaattgtt actccggcgc ggcgacgatt ggattcgaat cgacaaaccg gcggacgccc
     2761 gggtcgagac ctacacggat cagattcttt tgcggctgcg ctcggagtgg cgcgtcgacg
     2821 ggaaaaccta tagggccgga tcgctgctcg ccgccgattt cgcagcctat ctgcgcggcg
     2881 agcgcgagtt cgccgtgctg tttgaaccga gccaccgcaa agcgttggcg agcacgagcc
     2941 agacgaagaa tttcttgatt ctcaacgagc tcgacaatgt gcgcagcaaa gtttacgccc
     3001 tcaagcgcgc tgacgggcaa tggcagcgcc agcaattggc gaccccggct ttcggctcgg
     3061 tgcgcgtgcg cggcgtcgat gcccatgccg atgacgcctc ggatgattac ttcatgagca
     3121 taaccgactt cttgacgccg tcgagtctgg ttcttggcag agtcggcgcg ccgacagagg
     3181 aaaagctaaa aagcttgccg gcatttttcc gcgccgatgg tttggcgatt gaccagcatg
     3241 aagcggtatc gaaagacggc accaagattc cctactttca ggtgagccga aaggacctac
     3301 cgaccgacgg caagcatccg acactgctct acggctatgg cggcttcaac gtgtcgcagc
     3361 tgccgtttta cagcggcact ctcggcgcgg cctggctgga gcgcggcggc gtctacgtgc
     3421 tggcgaacat tcgcggcggc ggcgaattcg ggcccaactg gcactacgcg gcgcgcaagg
     3481 aaaaccgcca gcgcgcctac gacgacttca tcgccgtggc cgaggacttg atcgctcgta
     3541 aagtcacctc gccgcagcat ctcggcatcc agggcggttc caatggcggt ttgctggtgg
     3601 gagcggcgct gacgcagcgg ccggatttat tcaaagcggt ggtatgccag gtgccgctgc
     3661 tcgacatgcg ccgctacagc aagttgttgg ccggcgcgag ttggatcgat gagtatggcg
     3721 atcccgacaa agcagaagac tgggcgtata tcgcaaaata ttcgccctat cataatgtcg
     3781 ttgccgagaa gaaatatccg cgcgtgcttt tcaccacctc gacgcgcgac gaccgggtcc
     3841 atccgggaca cgcgcgtaaa atgttcgcca agatgaaggc gcagggccat gatgtgcttt
     3901 actatgaaaa tatcgaaggc ggtcacggcg gtgcggccga caatcgtcag ctcgcttaca
     3961 tgtcggcgct gacctatgcg tttctggtga aagagttgtt tggccagtga cgggatcgga
     4021 atattctcac gcaaagcgcg caaagaacgc caagggagga gaccaagggc agagcgagct
     4081 gtcggtcgcc cctacgcatc cgctcttgga aaattcaggg caccgacttc aaacgttctt
     4141 agctccggca taacctgctc ggtgaataag cgcagcgaat tgagcgcttg cttctgagtt
     4201 agctctccga aagtaaattc cccgagaaag gtgtcgccac cggcttggtt ggcgatggac
     4261 ttgatttgcc gcaccacggt gtccggcga
//
LOCUS       DASQKS010000070         8846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1298871, whole genome shotgun sequence.
ACCESSION   DASQKS010000070 DASQKS010000000
VERSION     DASQKS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298871"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="gltX"
                     /locus_tag="VE170_01820"
     CDS             complement(<1..1290)
                     /gene="gltX"
                     /locus_tag="VE170_01820"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYJ14194.1"
                     /translation="MAQVRTRFAPSPTGYLHIGGARTALFNFLFARQHGGKFILRIDD
                     TDRERSTPEAIQAILDAMEWLELDYDEGPFFQSERYPLYRQKVQELWAKGKAYPCTCS
                     AAELDSKRQAALKEKRKPSYDGTCRPAEGVIAALPEDKPYTIRFRTPREGTTVVNDLI
                     KGDVVFENRELDDLIIARTDGTPTYNFCVVVDDIDMEISHIIRGDDHLSNTPRQIHFY
                     QALGSPLPQFAHVPLILGTDKARLSKRHGATSVTAYRDMGFFAEAVVNYLVRLAWSHG
                     DQEIFSRQELLDMFSLEHVGKSAGVFNPEKFLWVNFHYLKSKPLSQLAEEVVPYIQAQ
                     GYPVPQDRQWLEKMIATLRERAKTLVELVEMARYYLDDQIVYEEKAATKFLNAAAADS
                     MTTLAGKLEGLAEFTEANIEQAFASTLEEHKLKMSELA"
     gene            1395..3098
                     /locus_tag="VE170_01825"
     CDS             1395..3098
                     /locus_tag="VE170_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HYJ14195.1"
                     /translation="MAEGDSSKGSSVNDSTPSHFIRNIVVEDIRQGKNQGRVYTRFPP
                     EPNGYLHIGHAKSICLNFGLAAEFGGKCNLRFDDTNPSKENVEYVDSIKEDVRWLGFA
                     WDGEYYASDYFERLYQFAIQLIHAGAAFVCDLNAEQMRQYRGTLTEPGKHSPYRDRSV
                     DENLDLFQHMRAGEVPDGGRTLRAKIDMASPNINLRDPVMYRVLRATHHRTGDNWCIY
                     PTYDFAHGQSDSIEGITHSICTLEFEDHRPLYDWFLDRLAVHHPQQIEFARLNLSHTI
                     LSKRKLLQLVQQKLVAGWDDPRMPTLAGIRRRGYTPEAIRNFCERIGVAKAYSTVDIA
                     MLEHSLREDLNKRAPRVMAVLRPIKVVLENYPEGQVEELEAVNNPEDPNAGTRKVPFS
                     RELYIEQDDFREVPPKGFFRLSPGTEVRLRYAYIIKCNQVVKDPSTGEVRELRCSYDT
                     ETKSGSSRSKRKVKATIHWVSASYAVEAEVRLYDHLFVVEDPDDVPEGADWLANINPN
                     SLERLTGCRVEPRLADAKVGERYQFERLGYFCVDADSTELRKVFNRSVTLRDAWAKAR
                     G"
     gene            complement(3161..4468)
                     /locus_tag="VE170_01830"
     CDS             complement(3161..4468)
                     /locus_tag="VE170_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HYJ14196.1"
                     /translation="MLKCSLTVVICIVSAIIVLCRAESTFGGGFTIPHQTARGLGLSN
                     ALTAGVNDPSAVYYNPAALGEVQGNVLLANGSYINVINDVENSGRNSVNKHDDNFLAT
                     LFGNYHIPGTDFTAGIGTYTPFGLATTYDSAITRFAAQRTELRTMYVTPAIAWHPSNQ
                     FSIGGGLSFVHASGLFTRALCFDSAACTAIVGSPAEAHLRLTDTQNAFTYNLGVLIKP
                     TDTLKLGLSYRARADIRFKDADVKLGGVGFAANPNAKADVKPLPLPPVINAGAFWQIT
                     PAWGAELVYEFTRWSEFKNFKANFAPIPLFFGLAPVPGFNLPQNWKDTSSVRFGTSYA
                     VNKTWEIRGGLSYDGGPTPNRTLNPSIPGGDSITLNTGIGYKWDRFGIDAGYMAVLYK
                     TRRVNNSELEGTPATGIPFNGAPGKDKYETFNNFLSLSASYRF"
     gene            4560..4751
                     /locus_tag="VE170_01835"
     CDS             4560..4751
                     /locus_tag="VE170_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ14197.1"
                     /translation="MQENLLTAEQLAYYLKVDKFTIYRLVARKKIPAFKVGNQWRFKQ
                     TLVDEWLMKNSNIESEDHR"
     gene            4801..6066
                     /locus_tag="VE170_01840"
     CDS             4801..6066
                     /locus_tag="VE170_01840"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYJ14198.1"
                     /translation="MSETLDPGQNRRVVITGLGVLSSIGIGKELFWDSLKNGKSGIKK
                     ITSFDTTSYECRIAGEISEFDPVDFMPSQTARRIDRFAQLAVAAARLAVTDAKLSIQS
                     ESPLRVGVILGTSIATLCYAEQQIALFHEKGVKRINPFFATSVIPSSGVTQITLDLGI
                     HGPSRTVTTACASGTASIGEAFRCVKSGDLDVILAGGAEAPITPLVISTLSSIQLLSV
                     ANDTPKSSYRPFSGDAAGFVLGEGASILVIEELGHALKRNASIYAEVVGFGSTSDAFH
                     VMEFSPHKEEPVMAIRQALAESHIEPSDLGYINPHGIAVPDNDRGEIEIFRKALGEAI
                     AHIPLSATKPMTGHVLGGGGALELAGCCLMLENDYLHPTINFSGSPASKGLDFIANVG
                     RATKVDAMLSVSFGFGGYNAACVIRRFCE"
     gene            6086..8251
                     /locus_tag="VE170_01845"
     CDS             6086..8251
                     /locus_tag="VE170_01845"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ14199.1"
                     /translation="MSFYSIPTFFTALLSLGLGVFILAKTANSKINGIFACWCLLTCY
                     WQICWTILFNVEDQGRAELIARLGYSGIIFIPIVFFHFVAEFVGSPGARRFAFLFYAL
                     GSLFLLAIWSTNTFVSGTYRYPWGFYPKGGPIHVGYLVFLVITLISGLTILHRFKQRS
                     VDQPTRLNQTKYIIVSTVIYSIAGVDFLANYGVGYYPMGLAFTNVHASIIAYSIIQYR
                     LLDVSVVIKRSLIYALLLLGLLLPCYFVVIQAQLYAFGRINYQFSLITLVLLTAAAFV
                     FPKFRFKTEDALERVLFKKRSDYRETLLRFSQDMVTIVDLKALSVKLVDTIGKALRIE
                     KVSLLLINEGKGSYTLEASVGLEFEHPERILLSRDGPLIKILQSRKEPIVKEELEWVP
                     VGPETLQTIETMGKLCAEISLPIILKEKLIGILNLGHKADRSLYSDEDLELLSTLGNQ
                     AAIAIENARLYENLKQSQVTLRRADRLSSLGLLTAGLAHEIRNPLVAIRTFTQLLPER
                     YNDAEFRDGFQGLALKEVDRICGLINDLLSFARPSKPNIAPENINDVVDNIARILEAQ
                     AKEKGVVIVRDFAVEVPKAWIDREQMKQVFMNLILNAIQAVDGEGSITISTREITRRG
                     TAAIGQFVQVEVRDTGVGIPDENLEHIFDPFFTSGKEEGSGLGLAVSHQIVEEHGGFL
                     TVESKVGQGTGFWVHLPVGAPARAGASGMDHTKEANLSH"
     gene            8621..>8846
                     /locus_tag="VE170_01850"
     CDS             8621..>8846
                     /locus_tag="VE170_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ14200.1"
                     /translation="MSTEKKTDASQPLILVVDDEFGVRQSLQMVFNKIYRILEASSAD
                     EAIQKVSEEKPDAVILDVMMPGTDGLGVLKQ"
BASE COUNT         2111 a         2175 c         2359 g         2201 t
ORIGIN      
        1 cgctagttcg ctcatcttta atttatgttc ttcgagcgta ctcgcaaagg cctgctcgat
       61 gttggcttcg gtgaattcag cgagcccctc tagtttaccg gccagggtgg tcatgctgtc
      121 ggctgctgcc gcgttcaaga actttgtcgc ggccttttct tcgtaaacaa tttgatcgtc
      181 gagatagtag cgcgccatct ccaccagctc gactaaagtt ttggcgcgct cgcgcaaagt
      241 cgcaatcatc ttttcgagcc actggcggtc ctgaggtacc ggatagccct gcgcttggat
      301 ataaggaacg acttcttctg cgagctgaga gagcggcttg gacttcaaat aatgaaaatt
      361 cacccagaga aatttttcgg gattgaaaac tccggcagac ttgccgacat gttccaagct
      421 aaacatgtcg agcaattcct ggcgcgaaaa tatctcctga tcaccatggg accaggcgag
      481 gcggaccaga tagttgacca cagcttcggc aaagaaaccc atgtcgcgat aagcggtgac
      541 cgaagtggcg ccatggcgct tgctcagcct agctttgtcg gtgcctaaaa taagcggcac
      601 atgagcgaac tgcggcagag ggctgcccag agcttggtaa aaatgaattt gccgcggcgt
      661 gtttgacaga tggtcgtcgc cgcggatgat atggctgatt tccatgtcga tgtcgtcgac
      721 gacgacacag aaattatacg tcggtgtgcc atctgttcgg gcgatgatga gatcgtcgag
      781 ctcgcggttt tcgaagacga catcaccttt gattaggtca ttgactaccg ttgtgccctc
      841 gcgcggtgtg cggaagcgga ttgtatacgg tttgtcttcc ggcagtgccg caataacgcc
      901 ttccgccggc cggcaggtgc cgtcatagga cggtttgcgc ttttctttca aagcggcttg
      961 gcgtttgctg tcgagctccg cggcgctgca agtgcagggg taggctttgc ccttggccca
     1021 gagttcttga accttttgcc gatacaacgg gtagcgctcg ctttgaaaaa acggcccttc
     1081 atcatagtcg agttcgagcc actccatcgc gtcgagaatc gcctggatcg cctcgggggt
     1141 cgaccgttcg cggtcggtgt catcgatgcg caggataaat ttgccgccat gttgacgggc
     1201 gaagaggaaa ttaaacagtg cggtgcgcgc gccgccgatg tggagatagc cggtcggact
     1261 gggcgcgaat cgggttctga cttgtgccat ttagagaagc taacaggggc atgaaatggc
     1321 gtcaacggcg gattatgaaa atgctgcaga tatgctagta tccgcggaat tacggcaatc
     1381 ctggggtagc taacatggca gagggagaca gtagcaaagg tagctcggtg aatgactcga
     1441 cgccttcgca ttttattcgc aacatcgttg tcgaggatat caggcaaggc aagaatcaag
     1501 gccgcgtcta tacccgattt ccgccggagc cgaacggcta cttgcacatc ggtcacgcca
     1561 aatcgatctg tctcaatttc ggcttggctg ccgagtttgg cggcaagtgt aacctgcgct
     1621 tcgacgacac caatccgagc aaggaaaacg tcgagtacgt cgattcgatc aaagaagacg
     1681 tccgctggtt agggtttgct tgggacggag aatattacgc ctcggattat ttcgagcggc
     1741 tctatcagtt cgccattcaa ttaattcacg ccggggcggc gtttgtttgc gatctcaacg
     1801 ctgagcagat gcgccagtat cgtggcacac tcaccgagcc aggcaagcac agcccctatc
     1861 gcgatcgttc ggttgacgag aatctagacc tctttcagca catgcgtgcc ggcgaagtgc
     1921 cggacggggg ccgcacgttg cgtgccaaga tcgacatggc atcgccgaat atcaatctgc
     1981 gcgatccggt tatgtaccgg gtgctacggg cgacccatca tcgcaccggg gataactggt
     2041 gcatttatcc gacctacgac ttcgcccacg gccaatctga ctccatcgaa ggcatcacgc
     2101 attcgatttg tacgctggag ttcgaggacc accggccgct ttacgattgg ttcttggatc
     2161 gcttggccgt gcatcatccg cagcagatcg aattcgcccg cctcaatctg agccatacga
     2221 tactaagcaa gcgtaagctc ttacagctag tccagcaaaa acttgtggcc ggctgggatg
     2281 atccgcgcat gccgacgttg gccggcatac gccggcgcgg ttatacgccg gaggccatcc
     2341 gtaatttctg cgaacggatc ggcgtggcca aagcctacag caccgtcgat atcgcgatgc
     2401 ttgagcatag tctgcgcgag gatctcaaca agcgcgcgcc gcgtgtcatg gcggtgttgc
     2461 ggccgatcaa agtcgttttg gaaaattatc ctgaaggtca ggtggaagag ttagaagcgg
     2521 tcaacaatcc tgaagacccg aacgcaggca cacgcaaagt cccgttctca cgcgagcttt
     2581 acatcgagca ggacgatttc cgtgaagtgc cgcccaaagg atttttccgg ctctcaccag
     2641 gcacagaggt ccgtctgcgt tacgcttaca tcatcaagtg caaccaagtc gtaaaagatc
     2701 cgagcaccgg tgaagtgcgc gagttgcgct gcagctacga cacggagacg aaaagcggct
     2761 cgtcgcgaag caagcgcaaa gtaaaagcaa caatccattg ggtatccgca tcttatgccg
     2821 tcgaagccga agtgcgactc tatgaccatc tgttcgtagt ggaagacccc gatgacgtac
     2881 cggaaggggc cgattggctt gctaacatca atcccaactc cctggaacgt ctcaccgggt
     2941 gtcgggtcga gcctcgtctc gctgatgcta aagtcggcga gcgctatcag tttgagcggc
     3001 tgggatattt ctgcgtcgat gccgattcaa cggaactgcg aaaagtgttc aatcggtccg
     3061 tgacactgag ggatgcgtgg gcgaaagcga ggggataagg tgaaagtttg ctgcggtctt
     3121 gggctaaggg ctgtgtctat acagagacgg tgaaatattt ttagaaacga tagcttgccg
     3181 atagcgacag gaagttattg aaggtttcat atttgtcttt tccgggtgct ccgttaaaag
     3241 ggattccggt cgcaggggtg ccttccaact cactgttatt gactcgtcta gtcttgtaaa
     3301 ggacggccat gtacccggca tcgatcccaa acctgtccca tttatagccg ataccggtgt
     3361 tgagagtaat cgaatctcca ccgggaattg acgggttcaa agtgcgattt ggcgtcggtc
     3421 cgccgtcata agaaagaccg ccacgaattt cccaggtttt gttaacagca taagaggtgc
     3481 caaatcttac gctactcgta tccttccaat tttgaggcaa attgaacccc ggaacgggtg
     3541 ccaaaccgaa aaataatggt attggtgcaa agtttgcctt gaaattttta aactcgctcc
     3601 aacgagtaaa ttcgtaaacc agttcagcgc cccaggctgg agtgatctgc cagaaggcac
     3661 cagcatttat aacaggcggg agcggcagcg gcttgacatc ggcttttgca ttagggtttg
     3721 ccgcgaatcc gacacctccg agtttaacgt ccgcgtcttt gaaccgaata tcggctcgtg
     3781 cacggtaact aagccccaat tttaaggtgt cggttggctt tatgagaacg ccgagattgt
     3841 aagtaaaggc attttgcgtg tcggttaatc gtaaatgcgc ttctgctggg ctaccaacaa
     3901 tcgcggtaca tgcagctgaa tcgaagcaaa gagcccgtgt gaaaagtcca gatgcatgaa
     3961 caaaactcaa accgccgccg atcgaaaatt ggttcgacgg atgccacgct attgcgggtg
     4021 ttacatacat cgttcgcaac tcggttcttt gggcggcaaa ccgggttatg gcgctgtcgt
     4081 atgtcgttgc cagtccgaat ggagtatagg ttccaatgcc cgcggtaaaa tcagtgcctg
     4141 ggatatgata gttgccaaac agtgtcgcga gaaaattatc atcgtgctta ttcaccgagt
     4201 ttctgccgct gttttcgacg tcattaatga cattaatgta gcttccgttc gcgagcaaaa
     4261 catttccttg aacttcacct aacgcagctg gattgtagta aacggctgaa ggatcgttaa
     4321 cgcctgccgt cagggcattt gatagcccca gacctcgtgc ggtttgatgg ggtatcgtaa
     4381 atccaccccc aaatgtcgac tcggctctgc acaggactat aatagcggac acgatacata
     4441 tcacgaccgt tagcgagcat ttgagcattt acgtctccac ggatcatcct tgacgataat
     4501 taccaaacct tatataccaa tgctaacaga aaacaactgc tgtcataagt gaggcatgaa
     4561 tgcaagaaaa tctgttaact gcagagcaat tggcgtatta cttaaaagtc gataagttca
     4621 caatttatcg actggtcgct cggaaaaaaa ttcccgcttt caaagtgggc aatcagtggc
     4681 gatttaagca aaccttagtc gacgagtggt tgatgaaaaa ctcaaatatt gagtcagaag
     4741 atcaccgcta gatatccttc aagcatcaaa ctctagagag gcgcgaaagg ttcgctaata
     4801 atgtccgaaa cgcttgaccc cggacaaaac cgccgtgtgg tgataacggg gctaggtgtt
     4861 ctatcgtcga ttggaattgg taaggaactc ttttgggatt cgctcaaaaa tggcaagtcg
     4921 ggtattaaga aaattacatc cttcgataca acttcatatg aatgcagaat agcgggggag
     4981 atttccgagt ttgacccagt cgatttcatg ccaagtcaga ccgcccggcg catcgatcga
     5041 tttgcccaat tagcggtggc tgccgcacgt ttggcggtta ctgatgcaaa actttcgatc
     5101 caaagtgaaa gtcctctgag agttggcgtg atcttaggta cctccatcgc gacgctatgt
     5161 tatgccgaac agcaaatcgc actttttcac gaaaagggtg tgaagagaat aaacccattt
     5221 ttcgccacgt ctgtgattcc gagcagtggc gtcacgcaaa tcactttaga cttagggatc
     5281 catggtccgt cccgcaccgt tacgactgct tgtgcctcgg gaacggcttc gatcggtgaa
     5341 gcgttccgtt gtgtcaagtc aggagacctc gacgtcatac ttgcaggtgg tgcggaagcg
     5401 ccgattacac ctctcgtgat atccacgtta tcgtcgattc aacttttgtc agtcgcgaac
     5461 gatactccta agtcttctta tcggccattt agcggcgatg ccgctggctt tgtgttggga
     5521 gaaggtgcga gcattctggt cattgaagaa ttaggccatg ccttgaagcg taatgcatcg
     5581 atttatgccg aggtcgtcgg ttttggctca acttcggatg ctttccatgt gatggagttt
     5641 tctccacata aggaagagcc ggttatggca atccgccaag ctctagcgga atctcacatc
     5701 gaaccgagtg atctgggata tatcaatcca catggaatcg ctgtgccaga taacgatcgt
     5761 ggcgaaatcg agatatttag aaaggcttta ggtgaggcga tcgcacatat tccattgagc
     5821 gcaacaaaac cgatgacggg ccatgtgctt gggggcggtg gggctttgga gctcgcaggt
     5881 tgttgcctca tgctggagaa tgactatctc catccgacga taaacttttc aggctccccg
     5941 gcaagcaaag gactcgattt catcgcgaat gtcgggcgcg cgacgaaggt tgacgccatg
     6001 ctatcggtat ccttcggctt cggcggttat aacgctgcat gtgtgatccg gcgattttgt
     6061 gagtaatttt aacgaggtat aaccgttgag cttctactcg attcctacct ttttcacggc
     6121 gctgctctca ctcggactcg gagtttttat cctcgctaag accgctaatt ctaagatcaa
     6181 tgggattttt gcatgttggt gcctgcttac ttgctattgg caaatctgct ggacgattct
     6241 ttttaacgtc gaggatcagg gacgcgcaga acttatcgcc agactagggt attccggtat
     6301 cattttcatt ccgatcgtct tttttcattt cgttgctgaa tttgttggaa gtcccggcgc
     6361 aagacgcttt gcctttttgt tttatgccct gggatcattg ttcctactag cgatctggag
     6421 cacgaacacc tttgtctccg gaacgtaccg atatccttgg gggttctatc cgaagggtgg
     6481 tccgattcat gtaggctacc ttgtgtttct ggttattacg ttaatctctg ggctcacgat
     6541 attgcatcgt ttcaagcagc gatctgtaga ccagcccacc aggcttaatc aaactaagta
     6601 catcattgtg tctacagtca tctactcgat agccggggtg gattttctcg ccaactatgg
     6661 tgtcggatac taccctatgg gcctggcttt cactaacgtt cacgccagta tcattgccta
     6721 ttcaatcatt cagtaccgac tgctggacgt cagtgtggtc atcaagagaa gcctgattta
     6781 tgccctactt ctcttaggct tattactccc gtgttacttt gtcgtgatcc aggcgcaatt
     6841 gtatgctttt ggtcgaatta attatcagtt ctccttgata actctcgttc tattgacggc
     6901 cgcggcattt gtcttcccaa aatttcgttt caagactgag gacgcgcttg aacgagtgct
     6961 gttcaaaaaa cgctccgatt accgcgaaac ccttctgcgc ttcagccaag acatggtcac
     7021 aatcgtcgat ctcaaagcgt tatccgtaaa actcgttgac actatcggca aggcactgcg
     7081 aattgaaaaa gtttcgcttt tgctcatcaa cgaaggaaag ggcagctaca ctttggaagc
     7141 gagcgttggt cttgagttcg aacatccgga acgaatactt ttgtcacgcg atggaccctt
     7201 gatcaaaatc ttgcagagcc gcaaagagcc gatcgtcaag gaagagctag agtgggtgcc
     7261 ggtggggccc gagacgctgc agacgattga gacgatgggc aagctttgcg cggaaatcag
     7321 tttgccgatc atattgaagg aaaagctcat tggtattttg aatttaggcc acaaagcgga
     7381 tagatctctc tactcggatg aagatttgga attgctttcg acccttggca atcaggcagc
     7441 gatcgccatc gaaaatgccc gcctttacga aaatctcaag caatcccaag tgacgttgcg
     7501 acgagccgat aggctttcgt cgttaggttt gctgacagcg gggttggccc atgagattcg
     7561 caatccctta gtggcgattc gcacctttac gcaactcttg cccgaacgct ataacgacgc
     7621 ggaattccgc gatggatttc agggactggc gctgaaagag gttgatcgga tttgcggact
     7681 tatcaacgac ttgctcagtt tcgcgcgccc gtccaagccc aatatcgcgc cggaaaatat
     7741 caacgatgtg gtcgacaaca tcgcgcgtat tttggaggct caggccaagg aaaagggtgt
     7801 tgtcatcgtt cgcgactttg ccgttgaggt tcccaaagcc tggattgata gagagcagat
     7861 gaagcaggta tttatgaacc tcatactcaa cgcgatccaa gcggtggacg gcgagggatc
     7921 cattacaatc tcgactcggg aaatcacgcg ccgcggtacg gccgcgattg ggcaatttgt
     7981 tcaggtggaa gtgcgcgata ctggtgtggg tattccggac gaaaacctcg agcacatttt
     8041 cgatccgttc tttactagtg gtaaggaaga aggcagcggc ttgggactgg ctgtgtcgca
     8101 tcagatcgtc gaggaacatg gcggttttct caccgtggag agtaaggtag gacagggcac
     8161 cggtttttgg gtacatttgc cggttggcgc tccggcacgg gctggagcga gcggtatgga
     8221 tcacaccaaa gaagcgaatc tcagtcattg agacgctcgc gctcgtcccg tggcaaatat
     8281 accactacca gccaagctca cgcccaatag gcttaacggc agacccgttt cgttcggatt
     8341 aataacagct gtcgcaccgt aaacaatcac cgttatattg tcattcttgt cactttttgc
     8401 ccatcgttga cagtgtcaac ggttattgtc ccgtaagcta actattggaa aagtaaaagc
     8461 tgccgattgg cctataggtt gctcatcatc ccgctggggg aatcgcagca catgttgagc
     8521 tagcacggcg cactgggcta tcacttgccg ccttgaaaga acccctaatc tggaaccgtc
     8581 gaggcgggaa aagatatgga tagaacggag ttaaggtttg ttgagcacgg aaaagaagac
     8641 tgacgccagt cagcctttaa tattagtggt ggacgacgaa ttcggcgtgc gccaatcgct
     8701 gcaaatggtc ttcaacaaga tttaccgaat cttggaagcc tcatctgccg atgaagctat
     8761 tcaaaaggtt tctgaggaaa aacccgacgc tgtgatttta gatgtcatga tgcctggcac
     8821 cgatggtctc ggtgtgctca agcaaa
//
LOCUS       DASQKS010000071         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4659217, whole genome shotgun sequence.
ACCESSION   DASQKS010000071 DASQKS010000000
VERSION     DASQKS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659217"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VE170_01855"
     CDS             complement(<1..702)
                     /locus_tag="VE170_01855"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ14201.1"
                     /translation="MNLLPTTAKFCILGAGSSGLAVAKNFRARGVPFDCLEREAEIGG
                     NWNFGKPNSSIYRSTRLISSKRLTEYVDYPMPKEWPEHPPHELVFQYLRDYAQHFGLY
                     ENIEFNTAVERIERAVASEPGAAGWDITLADGRRRHYAGIIIANGHNWDPRWPEFGGS
                     FAGEVIHSANYKEPVIARGRRVLVVGGGNSGFDIATDVAAHADMVFHSLRRGYHVLPR
                     FQRGTPIDQCGEWMLR"
     gene            1198..2301
                     /locus_tag="VE170_01860"
     CDS             1198..2301
                     /locus_tag="VE170_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14202.1"
                     /translation="MARKAISNDRYRLVGTYERLRKSLLEHPDDGRLDKSLSYWVLPT
                     DRRLPIAFLDRRLRDLLALPLDELMSTPGVGQKKILGFFDLLKRASKASSADLPFGMN
                     DGDAAKRASKPTPEASGFDSSVVSESLWSSWCETVVHFGMGAEKLGRLAPSLQALPTV
                     IWHSRLEEYSDRSLAEIRSLKTHGEKRVHAILEVFCTVHEALATATLQENIDVILVPR
                     FIPPLNRWLLDRVGLEELPSVDELHKNVVKPMLAQIRIDLGDQVAGLAAARLSLDDNA
                     PSVKQQADTIGVTRARVYQLLEDCAKVMDMRWPEGRWLLAPLATRFGTSDPDTIGLVH
                     GIMDLFYPVEKPPADLAGERQIAALSSFNPVED"
BASE COUNT          558 a          736 c          701 g          579 t
ORIGIN      
        1 gcgtagcatc cattcgccac actggtcgat cggcgtgccg cgttgaaatc gcggcagaac
       61 gtgatagcct cgccgaaggc tatgaaacac catgtcagcg tgcgcagcca cgtcggtcgc
      121 aatgtcaaaa cccgagttgc cgcccccgac aaccagcacc ctacgcccgc gcgctatgac
      181 tggctcctta taattcgccg aatgaattac ctcgcccgca aaacttccgc caaactccgg
      241 ccaacgcgga tcccaattgt gcccgttcgc aatgatgata ccggcgtagt gccggcgtct
      301 gccgtcagcc aaggtaatgt cccaaccagc ggcgcctggc tcgctggcga ccgcgcgttc
      361 gattcgctcg actgccgtgt tgaactcaat attttcgtaa agtccaaaat gctgtgcgta
      421 gtcccgcaag tactggaaca ccaactcatg cggcggatgc tcaggccatt cctttggcat
      481 aggatagtcg acgtactccg tcaatcgctt tgatgatatc aagcgtgttg aacgatagat
      541 gctgctattc ggtttgccaa agttccaatt gccgccaatt tccgcttcac gctcgaggca
      601 atcaaacgga actccccgcg cgcggaaatt cttcgccacg gccaggccgg aggagccggc
      661 gccgagaatg caaaatttcg ctgttgttgg cagcagattc attggtgttg acgcccagcc
      721 gtcgcgccga tgcggcatgt cctaaatggc gctgattgcg gacactttca cttaagcgtc
      781 atcgtgttgt cgacatgtcc aacgggcaac gcgcccgctc aaacaaaaaa tccgactttt
      841 ttccaatggt gaaagtctgc attaccgaag gtaaccgaag cgagacattc ggcagtgaaa
      901 acttgcaaaa acacatattt tcacagaatt ttcgaagaag gttgccagat taggcgggtg
      961 ctagtggcgc cgactccgca agttttccgc ggcaatccct gcttgacagt ttgacgtggc
     1021 agacaagaat tttatccaga tttgaacccc gcccgcgaga ggcggggttt tacccatgtg
     1081 tagaagcatc ggcaggaaat cgatgccagc accgtgggcg agtcgggcaa tccttggggc
     1141 gacacccgtt tgattcttta agcaggcgag tttctttgac tatggagcat aaccgcaatg
     1201 gccaggaaag ccatttctaa cgaccgttat cgactagtag gcacttacga aaggcttcgt
     1261 aagagtctgt tggaacatcc tgacgatggg cgcctcgaca agtcattgtc gtattgggtt
     1321 ctgcccaccg atcggcggtt gccgatcgcg tttctcgatc ggcgattgcg cgatttgtta
     1381 gcgctgccgc tggatgaatt gatgtcgacg cccggcgttg gccaaaagaa aattttgggc
     1441 ttcttcgatt tgctaaaacg ggcgtccaag gccagctcgg ccgacctacc gttcggaatg
     1501 aacgatgggg atgcagccaa gcgggcctcc aaaccaaccc ctgaggccag cggctttgat
     1561 tcatcggtcg tgtccgaatc tttgtggtca agctggtgcg aaaccgtcgt ccatttcggc
     1621 atgggcgcgg aaaagctcgg acgcttggcg ccctcgttgc aagcgttgcc caccgtgatc
     1681 tggcactcgc gcctcgaaga atattccgat cgctcgctgg ctgaaattcg ttcgctcaaa
     1741 acgcacggag aaaagcgcgt ccatgcgatt ttagaagtct tctgcaccgt ccatgaagcg
     1801 ctcgccacgg ccacactgca agaaaacatc gatgtaattc tagtgccgcg attcattccg
     1861 ccattgaacc gctggctgct cgatcgagtc ggcttggagg agctgccttc cgtcgatgag
     1921 cttcacaaaa acgtcgtcaa gccgatgctc gcgcagattc gcatcgattt gggcgatcaa
     1981 gtggccggcc tggctgcggc ccgactaagt ctcgacgata acgcgcccag cgtcaaacaa
     2041 caggccgaca cgatcggcgt aacacgggct cgcgtttatc aactgttgga agactgcgcg
     2101 aaggtgatgg acatgcgctg gccggaaggt cgttggctgc tcgcgccgct cgccacgcgc
     2161 ttcggcacgt ccgatccgga tacgatcggc ctggttcatg gcatcatgga cctgttctac
     2221 cctgtagaaa agccgccggc agatttagcc ggcgaacgcc aaattgcggc actaagctcc
     2281 ttcaatcccg tcgaagatta gccgctgctt ttattttaat tgacgaatcg acgcgatacg
     2341 gaagctttac tctggctttc tcctcgcgca ccttggtatc tgtgtctttt tctcggccga
     2401 aacttttttc tggttaatcg ccatagcggc ctgcggtctt ctggttaggg acacaattcc
     2461 tttccaggag atcgaaacat gttgcgagag cacttgagct cggggatcgc gcacgtgggg
     2521 ctcgcccttc gcgatcccga agactttgcg gccgcgtgga accggggcga aacc
//
LOCUS       DASQKS010000072         6351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1299651, whole genome shotgun sequence.
ACCESSION   DASQKS010000072 DASQKS010000000
VERSION     DASQKS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299651"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1651)
                     /locus_tag="VE170_01865"
     CDS             complement(227..1651)
                     /locus_tag="VE170_01865"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ14203.1"
                     /translation="MDRAPRIAQEIREEVLRRWVTPLTSRLVETSENARAPLRNLEAL
                     IKARPALSDRLVLLAHLATGSAPRLSGFAQATRTLGPDDVKPLILALLAYEPRPLGPS
                     QSKALFDPDPTTLRELWEHALASAMIAAKIATKLANVSPMQAFTAGLIHDIGRVLLWR
                     YSNVEFAAAVALAHQQQLPIGQAETSAIGIDHTTMGDAWCGNCKIAAPLAEMVRRHHE
                     PITVVDATVTAGVPSLIAIVQAAESLSETAPLGVNDEGLPASADGWLSLGLNKAEWAE
                     TVQSFTAQIETLREAFGFRRFDSAKPNCFRREISPPPPAAPLPASTSSLAGRGRVIPF
                     PMSAAAARRFEARTPAEKLVLLVIEDHGTLCEMVSLYLMRCGYHVRTASNGVMALEIL
                     AREEIHMVLLDLMLPRIDGFTVLRQIHNSRREMLPYIIVVSAGVSEHDRRRVFDLGAN
                     EYLPKPFQLTRLLERIQAVEKYLL"
     gene            1786..2643
                     /gene="motA"
                     /locus_tag="VE170_01870"
     CDS             1786..2643
                     /gene="motA"
                     /locus_tag="VE170_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HYJ14204.1"
                     /translation="MFAIIGYAIVFGSVLGGFVIEGGAVGILVQPVEFLIICGAALGA
                     LIIGTPIKTIKAMIASIMNAIMKNGYSKADYMELFAMMFEVFSVMRKSGEMAMEKDVD
                     DPGNSEIFKKYPKFMSNHGAKSMLLDSLRLVISGSANAEELDNLMDEEIATHEEEGKR
                     PIEVLTKTADAMPGLGIVAAVLGVVIAMGVMDQGPEVLGHKIAVALVGTFIGILVSYA
                     MLQPLCQKMELLLVEEGKYLQCIKSGILAYLHGAAPIIAVEHARRGVFTTERPGAQEL
                     EDACRGKKE"
     gene            2649..3584
                     /locus_tag="VE170_01875"
     CDS             2649..3584
                     /locus_tag="VE170_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HYJ14205.1"
                     /translation="MAAAKGGKAETAKHGKDDKPRVIAKKCRLPHGGGHHGGAWKVAY
                     ADFVTAMMALFLVLWLLGASNSKEKAVIAHYFREPGVFSGSRGIIPNGGQDKLGEGII
                     QAPSMETLHANIQEGLSALKESLNVTDQVPITITADGLLIEMIDKEKQAFFEVNSANV
                     KPIMRKILSLIAKEIKAAPNLLIVAGHTDARGFHDDSFYSNWELSSARALNTRRDLEE
                     LGIASQRFEQVVGYADRKLRLPNDPLAAENRRISILIKNVDKSDASKTEWKATTKAPD
                     QPHVEKSEMKSTEKARAEKPATKPAEKDHAAKAAH"
     gene            complement(3863..4171)
                     /gene="fliN"
                     /locus_tag="VE170_01880"
     CDS             complement(3863..4171)
                     /gene="fliN"
                     /locus_tag="VE170_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HYJ14206.1"
                     /translation="MDEVSNDKNSGRAANDQFDLAMLMDVPLKVTVELGRTRMTIENL
                     LKLNQGSVVELSQMIGEPLDILVNNKLMAHGEAVVIKDKFAVRILDVVSPERRIENLG
                     "
     gene            complement(4209..5219)
                     /locus_tag="VE170_01885"
     CDS             complement(4209..5219)
                     /locus_tag="VE170_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HYJ14207.1"
                     /translation="MAQVLSQDEIDALLGGIATQQIATSDTAATYGQTEPESATPPAR
                     SSEFKTYDFSRSDISNRGRLPGLEVILADFSRRLQSLFASQLGKSVDVSYDGMEVVAY
                     GTLIQSLPLPGSIHVVRLEPLRGMGMMVMEARIAFAMIELFFGGTGTKTMKVEGRDFT
                     TIENSFLGKFVAHMLKGMEESWQSVIALKTRYVRAELNPYLLNVASTADPMILATYTV
                     NMSPISGSIIFALPLAAIEELRNQLKVSVQLDDNPDNLGLFKRARQPLLGVEVEVRAV
                     VDVINMSLGEIMSLHAGDVIQLNVAGLDDVELWVEGKRKFIGKGAQRNGNKVFVNARA
                     CE"
     gene            complement(5507..>6351)
                     /locus_tag="VE170_01890"
     CDS             complement(5507..>6351)
                     /locus_tag="VE170_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ14208.1"
                     /translation="RVLVVDDLVVNRKILKHQLESQGMSMVGAASAMDALEILTTKEN
                     FDVAILDMHMPEMDGIKLATKISALAHRRLLPLIMLSSMGQRDNANELFTAVLTKPAK
                     EALLFDALSALFDAGSNAKARTAAIDTTLGSRHPLRILLAEDNVINQKVALKILERMG
                     YQADVAHNGKEAVDAVARQTYDVVLMDVQMPEMDGVQATAQIRAQFGDNRPWIVALTA
                     NALDGDRERYIGVGMDDYISKPINVDELAKALEQAMLRTLPAPRAGLNLPDTMTKTDT
                     LRIL"
BASE COUNT         1415 a         1792 c         1748 g         1396 t
ORIGIN      
        1 acgactatga tgatgatttt tttcatggcg ttgtctcgct gccgataaca gtgactcagc
       61 aacgcacatg ccatgggcta gaccctgaaa atgattgaaa tttttgtttt atgtcgaggg
      121 tggccaggcg gtcggcgtca acaacaccga tggccgcgtc agagttttga cgcagaccat
      181 aaatcggcca ttgggatgcg ggctgtcgcg aatgcgccgc ggcgcatcag agcagatact
      241 tttccaccgc ctgtatgcgt tcgagcaaac gcgtcaactg aaacggctta ggcagatatt
      301 cgttggcgcc aagatcgaaa actcttctgc ggtcgtgttc cgatacaccg gcggatacca
      361 cgataatgta cggcagcatt tctcgacggc tgttatgaat ctgccgcaac acagtaaaac
      421 cgtcgatccg cggcagcatc aaatcgagca gcaccatgtg aatctcttcc ctggccaaga
      481 tttccaaggc catgacgccg ttgctggccg tgcgcacatg ataaccgcag cgcatgagat
      541 ataaactgac catttcgcac agcgtcccat ggtcttcgat caccagcaaa acaagctttt
      601 cggctggggt gcgcgcttcg aagcggcgcg ctgctgcagc cgacatggga aacggaatca
      661 ctcggccgcg gccagccaag ctggaggtag atgcgggcag cggcgcagcc ggaggcggcg
      721 gagatatctc gcggcgaaag caattcggct tggcgctgtc gaaacggcga aagccgaagg
      781 cctcgcgcag ggtttcgatc tgcgccgtaa agctctgtac ggtctcggcc cattccgctt
      841 tgttgaggcc aagcgacaac caaccgtcgg cggacgctgg caagccttcg tcattgacgc
      901 cgaggggcgc tgtttcgctt agcgattcgg cggcctgaac gatggcgatc aagctcggca
      961 cgccggcggt gacagtagcg tcgaccaccg tgatcggctc atggtgccgg cgcaccattt
     1021 cggccagggg cgcggcgatt ttgcagttgc cacaccaagc gtcgcccatc gtagtatggt
     1081 cgatgccgat ggcgctggtc tccgcttgac cgataggcag ctgctgctgg tgcgctaacg
     1141 ccaccgcggc agcgaactct acattggaat agcgccacag caataccctg ccgatgtcgt
     1201 gaatcaagcc ggcggtgaag gcttgcatgg gcgaaacatt ggcgagcttg gtggcgatct
     1261 tggccgcgat catggcgctg gccaaggcat gttcccacaa ttcacgaagc gtcgtcggat
     1321 ctggatcgaa aagcgcttta ctctgggacg gtccaagcgg tcttggttca taggcgagca
     1381 gcgcgaggat caacggctta acatcgtccg gaccgagggt gcgggtcgct tgcgcgaatc
     1441 cgcttaatcg gggcgccgaa ccggtggcga gatgggccag gagcaccagc cggtccgaga
     1501 gagccggtcg cgcttttatc aaagcttcca agttgcgcaa aggcgcgcgc gcgttttcgc
     1561 tcgtctcgac gagtcgtgac gtcagcggcg tgacccagcg gcgcaagacc tcctcgcgta
     1621 tttcctgggc gattctaggt gctctatcca tccctatttc gcatcggtcc aaagcacgaa
     1681 aatctttagt tttgttaaag aatcgccgaa cttggacgat tgattcaaca tggcggaatc
     1741 gagctagcgg cctcgaccca tgtctgagag aggggttacg aacctgtgtt tgccatcatt
     1801 ggatatgcaa tcgtcttcgg ttcggtgctc ggtggctttg tcatcgaggg tggcgccgtc
     1861 ggcatcctcg tgcaacccgt tgagtttcta attatctgcg gcgccgcctt gggtgcattg
     1921 atcatcggca cgccgatcaa gaccatcaag gcgatgattg ccagtatcat gaacgccatt
     1981 atgaagaacg gctacagcaa agccgactat atggaactgt tcgctatgat gtttgaggtg
     2041 ttttcggtga tgcgtaaaag cggcgaaatg gcgatggaaa aggacgttga tgatccgggc
     2101 aatagcgaaa tcttcaagaa atatccaaaa tttatgtcca atcacggcgc gaaaagcatg
     2161 ttgctcgatt ccttgcgcct ggttatcagc ggctcggcca acgccgagga actcgataat
     2221 ctaatggatg aggagatcgc gacccacgaa gaagaaggca agcggcccat cgaagttttg
     2281 accaaaaccg ccgacgcgat gcccggcctc ggcattgtcg ctgccgtctt aggtgtcgtc
     2341 atcgctatgg gagtcatgga ccaaggaccg gaagtgctcg gtcacaagat cgccgtcgct
     2401 ctggtcggca cgtttatcgg tattttggtc tcctatgcca tgctccagcc gctctgtcaa
     2461 aaaatggagc ttctgctcgt cgaagaaggg aagtatctcc agtgcatcaa gtctggcatt
     2521 ctcgcctatc tgcatggcgc ggcgccgatt attgcagtcg aacacgcccg gcgcggggtt
     2581 ttcaccactg agcgtcccgg cgcccaagag ctcgaagacg cctgtcgcgg taagaaagag
     2641 taatccatat ggcagcagcc aagggcggca aagccgaaac ggcgaagcat ggcaaagacg
     2701 acaagccgag agtcatcgca aaaaaatgcc gcctgcccca cggaggtggc catcacggtg
     2761 gcgcctggaa agtcgcttac gccgacttcg tcacggccat gatggcgttg tttctcgtgc
     2821 tctggctcct aggagcgtct aacagcaaag aaaaagcagt catcgcccac tacttccgtg
     2881 agcctggcgt attcagcggt tcacgcggca ttattcccaa cggcggccaa gacaaactcg
     2941 gcgaaggcat cattcaggcg ccgtccatgg aaacgctcca cgccaatatc caagaaggtt
     3001 tgtcagcctt gaaggagagc ctcaacgtca ccgatcaagt gccgatcacg atcaccgccg
     3061 atggcctgtt gattgaaatg attgacaaag agaagcaggc attcttcgag gtcaacagcg
     3121 ccaacgtcaa accgatcatg cgcaagatct tgagcttgat cgccaaagag atcaaagctg
     3181 cgcccaattt gttgatcgtc gccggtcata ccgacgcgcg cggctttcac gacgattcgt
     3241 tttattccaa ctgggagttg tcgtcggcgc gggcacttaa tacccgacgc gatctcgagg
     3301 agcttggcat cgcgtcacag cgtttcgagc aagtcgtcgg ttacgccgat cgtaagttgc
     3361 gcctgcccaa cgatccacta gctgccgaga atcgccgcat cagcattctc atcaaaaatg
     3421 tggacaagag tgacgcgagc aaaactgaat ggaaagcgac aactaaagcg cctgaccagc
     3481 cccacgttga gaagtctgag atgaaatcca ccgagaaagc gcgcgcagaa aagcccgcaa
     3541 cgaaacccgc tgaaaaagac cacgccgcca aggctgcgca ttgataaggc agcgaaactg
     3601 atttgtctgt cgagatcgat tgaccgacca ctaaaccgca gcactgcaac ggttggcgcc
     3661 ctcggcatat atggcgcatt tttcaatcaa ctgaagatgg cggacggcgg atttgacttg
     3721 ttggagatgc tcggtggtaa acggcgatat tcccgcgctg cagacgacca agggttcatc
     3781 caaatttttt tcgaagacca gtttctactg attcgttacc gacgcagcgg aacgacatta
     3841 agttgattca gctgcgttga acttatccaa gattttcgat gcgccgctcg gggctgacca
     3901 cgtcgaggat gcgcaccgcg aacttgtctt tgatcaccac ggcttcaccg tgggccatca
     3961 atttattgtt aaccagtatg tcgaggggct cgccgatcat ttggctcaac tccaccaccg
     4021 aaccctgatt gagcttgagc aaattttcga ttgtcatgcg ggtacgcccg agttccactg
     4081 tcaccttgag cggcacgtcc attagcatcg ctaggtcgaa ctggtcgttg gccgcccggc
     4141 cgctattttt atcgttgctc acttcgtcca ttgggtgaat ccttctccgt cctactacct
     4201 tgccgatgct actcgcacgc gcgcgcgttg acgaacacct tgttaccgtt tcgttgcgcc
     4261 cctttgccga taaatttacg cttgccttcg acccacagct ccacatcatc gagtccggcg
     4321 acattcaatt gaatgacatc gccggcatgc aaactcatga tctcgcccag gctcatattg
     4381 atcacgtcga ccaccgctcg gacctcgacc tcgacgccga gcaagggctg gcgcgcgcgt
     4441 ttgaacaatc cgagattatc cggattatcg tccagttgga cgctcacttt cagctggttg
     4501 cgcagctctt caatggccgc cagcggcagt gcgaatataa tcgacccgct tatcggcgac
     4561 atattgaccg tgtacgtagc caagatcatc ggatcagccg tgctggcgac gtttaagaga
     4621 tagggattta gttccgcccg gacataccgg gtcttgaggg caatgaccga ttgccacgac
     4681 tcctccatgc ccttgagcat atgcgcgacg aatttgccga gaaaggaatt ttcaatggtg
     4741 gtaaaatcac gcccctctac cttcatcgtt ttggtgccgg taccgccgaa aaacaattcg
     4801 atcatggcaa aagcgatgcg cgcttccatg accatcatgc ccatcccgcg caacggctcg
     4861 agacgcacga catggatgct ccctggcagg ggcaagcttt ggatcagagt cccataggcg
     4921 accacttcca tcccgtcata actaacgtct acggatttgc ccaactgcga agcgaacaag
     4981 ctttgcagcc gacgggaaaa atcggcgagg atgacctcga gaccaggcag gcgtccacga
     5041 ttggaaatat cactgcggga aaaatcatag gttttgaact cactgctccg cgctggtgga
     5101 gtggcgcttt cgggctcagt ctgcccatac gtcgcggcgg tgtcggacgt ggctatctgc
     5161 tgggtagcga taccaccaag cagtgcgtcg atttcgtctt gggacagaac ttgggccata
     5221 ggtactaacg actccactga ggcttgcgcc ggcgccaacg ttttgacgct acattgtcaa
     5281 ctcttaagcg ggaaaagccc gtttactcta aaatgtcagc ttccgggatt catgagcaag
     5341 tgaagtgcca gagcgccgga actttctggc cgtgtcggtt cagcacctgg aggaacttct
     5401 tagacgtccg acggatcttg ttgagattag ccggctgtag gtgtagccag attagcctat
     5461 ccgtgtatcg tccgctcatc cataattatc tcatcgcaac atccagctaa agtatcctca
     5521 aggtatcggt tttagtcatg gtgtcgggca ggttgagtcc ggcacgcggc gccggcaaag
     5581 tcctgagcat cgcttgttcc aatgccttag ctaactcgtc cacgttgatc ggcttgctga
     5641 tatagtcgtc catgccgacg ccaatatagc gttcgcggtc gccgtctaat gcgttggccg
     5701 taagagcgac gatccacgga cggttgtcgc caaactgggc tctaatctgg gccgtcgctt
     5761 gcactccgtc catctccggc atctgcacgt ccatcaacac cacatcataa gtctgccgtg
     5821 ccaccgcatc gaccgcttcc ttgccgttgt gcgcgacatc ggcctggtaa cccatccgtt
     5881 cgaggatctt aagcgccacc ttctggttta tgacgttgtc ttcggcaagc aaaatacgca
     5941 gcggatggcg tgacccgaga gtggtgtcga tcgctgcggt tctggctttc gcattcgacc
     6001 ccgcgtcaaa tagcgccgat aaagcgtcga agagcagcgc ctccttggcc ggcttggtaa
     6061 gcactgcggt aaatagctcg ttggcattgt cccgctgccc cattgaactc agcatgatca
     6121 gcggaagcaa ccggcgatgt gccagcgcgc taatcttagt cgccagcttg atgccgtcca
     6181 tttccggcat atgcatgtcg aggatcgcca cgtcaaagtt ttctttcgtc gttaagattt
     6241 ccagagcatc cattgcagac gctgccccaa ccatcgacat tccctgggat tccagttggt
     6301 gcttgagaat cttgcggttg accaccaaat cgtcgaccac gagcacgcgc t
//
LOCUS       DASQKS010000073         4511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1720584, whole genome shotgun sequence.
ACCESSION   DASQKS010000073 DASQKS010000000
VERSION     DASQKS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720584"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VE170_01895"
     CDS             <1..1059
                     /locus_tag="VE170_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14209.1"
                     /translation="KLKNRDTRLDVFVMDVIWPAEFASAGWALPLDRYFTSDEQKKFL
                     SAPLAANQYGGQIFGVPLFIDAGLLYYRKDLLAKHKLAPPRTWLDLVESAKIVLAREH
                     DPQLVGFSAQFKQYEGLVCNMMEFILGNGGALWDPVRMVSTLDQPAALTAMGFVRDHI
                     VGEISHRGVLAYEEPESLALFTQGLAIFHRNWPYAWAIANDPAKSQVAGRVAMSPLPG
                     LSPGKGVAALGGWQFGISRFSNKPDLAWRFAAHMTGAAMQKRIALATGRAPTRVALYD
                     DPEIKRQIQQLPALLDTFKQAVPRPATPVYAPLSNIMQRYFSSVLALPNTDINKRAAL
                     AARDMNRALDLLRDRRSP"
     gene            1056..1943
                     /locus_tag="VE170_01900"
     CDS             1056..1943
                     /locus_tag="VE170_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYJ14210.1"
                     /translation="MKGSQRLGAIFLLPSFLFVAIFSIFPIVESFRLSFHRLILTLPW
                     LGQKMVGWENYSDLASDPVALAATVTTIIFIGVTVSLELLFGLAIALILNEAFHGRSL
                     LRAVVLIPWAIPTVVSSQMWRFIFNDRYGLANLMLFGSDTSRYLAPLADSYFAPIAIM
                     IAEVWKTTPFAALIILAGLQTIADELYEAASIDGATWWQKFRLITLPLLKPALLLALL
                     FRTIDALRVFDLVFVMTQGGPADATNVLQFYGYKKTFAEGMIGYGSAIAVAVFSMSLV
                     LSLAYLHAFRTNRLNGTRP"
     gene            1940..2755
                     /locus_tag="VE170_01905"
     CDS             1940..2755
                     /locus_tag="VE170_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYJ14211.1"
                     /translation="MKRSIALVFFSVGIALYCLVPFLWFVLTSLKSSAELTAIPPILW
                     PSFHWSFYQSALQDYGLLHYVLNSAIVAGSATLVTVTVGSLAGYAIARFHLTWTNFYL
                     LFLLAVSMFPQIAIAGPIWTILDRLQWLNTFQGLVVAYVALSLPLAIWILTTFFREVP
                     REIEEAALIDGCTRLQALYKIVLPLAAPGILTAALLVFIHAWNEFFFALIIMTDPAVQ
                     TLPVGIALFPGEYTMPWGEIAAASTIATIPLILLTLIFQRGIVRGLSAGAVKG"
     gene            2739..3854
                     /gene="ugpC"
                     /locus_tag="VE170_01910"
     CDS             2739..3854
                     /gene="ugpC"
                     /locus_tag="VE170_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYJ14212.1"
                     /translation="MRSKVDTYGPGGFIAGLSVKQISKRFGAAEVLQDVSFDVADGAF
                     CILVGPSGCGKSTLLRLIAGLDQPSGGEIFIGAERVDALAPRDRDVAFVFQNYALYPH
                     LTVFENLAFSLRLRGVAKIEIGQKVKEAARLLEIEDLLERRPQALSGGQRQRVALGRA
                     IVRQPRIFLFDEPLSNLDAALRAGMRVELARLHHRLGTTIVYVTHDQAEALTLGEQII
                     VLDRGIIQQIGSAASVYHTPANVMVAGFVGSPQMNLLAGDVDTDGAVFQCANLRLDLT
                     HVLKKSRAQRLPRSLTIGIRAEDFAIAEPAAAWITGDIDLIEDLGSDRYLRVKCDRIE
                     LIVRAGRELKVQRGDRIGLNVSVDRMHFFADGKRYEL"
     gene            3964..>4511
                     /locus_tag="VE170_01915"
     CDS             3964..>4511
                     /locus_tag="VE170_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ14213.1"
                     /translation="MKMINRDLSADKDGVPGYLAHPERKDRGPGVLIVHHHYGVTGNM
                     KVNVCNFAKLGYTTVVPDLYKMLGESDGVHDAQKKTTDGQFIEILNRSWNYLTSRPEV
                     DAKRCAVVGYCMGGRIGIHFVAATPSVRAFVGYYPSVRDEEPSKLRPRHPNDAVKEFN
                     CPSQIFLGGKDHVASISVQQKL"
BASE COUNT          872 a         1348 c         1242 g         1049 t
ORIGIN      
        1 aaattgaaaa atcgggatac ccgactcgac gtttttgtca tggatgtcat ctggccggcg
       61 gagttcgcat cggccggttg ggccttaccg ctggatcgct atttcacctc ggatgaacag
      121 aaaaagtttc ttagcgcgcc actggcggcc aatcaatacg gcggacaaat cttcggtgtg
      181 ccgctgttta tcgacgctgg gctgctgtac taccgcaaag atctcttggc aaaacataag
      241 ctggcgccgc cgcgcacttg gctcgatctc gtcgaaagcg ccaaaatcgt cctcgcccgc
      301 gagcacgacc cgcaactcgt cggtttctcc gctcagttca agcaatacga agggctcgtc
      361 tgcaacatga tggaatttat tctcggcaat ggcggcgccc tatgggatcc agtccgcatg
      421 gtgagcaccc tcgatcaacc ggctgcgctg accgccatgg gtttcgtccg cgaccatatc
      481 gtcggagaga tttcccaccg cggcgtcctt gcatacgagg agccggaatc gcttgcgctg
      541 tttacccagg ggctggcaat ttttcaccgc aactggccct acgcttgggc catcgctaat
      601 gacccggcga aatcacaggt cgcaggcagg gtcgcgatga gcccgctgcc gggactttct
      661 cccggcaagg gagtcgccgc gctgggtgga tggcaattcg gcatcagccg attctcgaac
      721 aaacctgacc tggcctggcg gttcgcggcc catatgaccg gcgcagcaat gcaaaagcgc
      781 atcgcgctgg cgaccggtcg ggcgccgacc cgtgtggccc tttacgatga cccggaaatc
      841 aagaggcaga tccaacagtt gccagcgctg ctggacacgt tcaagcaagc cgtgccgcgt
      901 ccggcgacac cggtctacgc cccgttgtcg aatatcatgc agcgctattt cagctcagtg
      961 ctggcgctgc cgaataccga catcaacaag cgtgccgcgc tcgctgcgcg cgacatgaat
     1021 cgcgccctgg atcttctgcg cgacaggcgg tctccatgaa aggttcgcag cgccttggag
     1081 cgatctttct gttgccaagc tttttgtttg tcgcgatctt ctccatcttc ccgatcgtcg
     1141 aatcgtttcg cctgagtttc catcggctga tcctgactct accttggctc ggtcagaaaa
     1201 tggttggctg ggagaactac agcgatttag cgagcgatcc cgtggcgctc gcggcgaccg
     1261 tgacgaccat catattcatc ggcgtgactg tttctttgga attgcttttc ggtttggcca
     1321 tcgctttgat tttaaacgaa gcctttcacg gccggagcct tttgcgggca gtggttctca
     1381 ttccatgggc aattccgaca gtggtttctt cgcaaatgtg gcgtttcata tttaacgacc
     1441 gttacggcct cgccaatcta atgcttttcg gcagcgacac atcgcgctat cttgccccct
     1501 tggccgattc gtattttgca ccgatagcga tcatgattgc cgaggtttgg aagaccacgc
     1561 ccttcgcggc gctgattatt ttggccggcc tacaaaccat tgccgacgaa ctttacgagg
     1621 cggcaagcat cgacggcgcc acgtggtggc aaaagtttcg cctgatcacc ttgccgctgc
     1681 tcaagcctgc gctgcttctg gcactgttgt ttcgcacaat cgatgcccta cgggtgttcg
     1741 atctggtctt tgtcatgacc cagggcggac cggctgacgc gaccaatgta ctgcaattct
     1801 acggctacaa aaaaaccttc gcggaaggga tgattggcta cggttcggcc atcgccgtgg
     1861 cggtgttttc gatgtcgttg gttttgtcgc ttgcctatct gcacgcattc aggacaaacc
     1921 gtttgaacgg gacaaggccg tgaaacgttc catcgctctc gttttttttt ccgtcggcat
     1981 tgcgctctac tgcctggtgc cgtttctctg gttcgttttg acttcgctca agagtagcgc
     2041 ggagctgacc gcgattccgc ccatcttatg gccctcgttt cactggagct tctatcaatc
     2101 ggcgttgcag gattatggat tgctccatta cgtcctcaat agcgccatcg tcgccggctc
     2161 ggccacactg gtcaccgtca ccgtcggttc gctggcgggt tatgccatcg cccgctttca
     2221 tttgacttgg acgaactttt atctgctctt cctgctcgcc gtttccatgt ttccccagat
     2281 cgccatcgcc ggtccgatct ggaccatcct cgaccggctt caatggctca acacctttca
     2341 aggcctggtg gtagcgtacg tcgccctgtc attgcccctg gcgatctgga ttctgacgac
     2401 gtttttccgt gaagtgccga gggaaatcga agaggccgcg ttgattgacg gctgcacccg
     2461 gctgcaagcc ctgtacaaga tcgtgttgcc gctcgcggcg ccgggcattc tgaccgccgc
     2521 gctgctggtc ttcattcatg catggaacga gtttttcttc gcgttgatta tcatgaccga
     2581 cccggcggtc cagactctgc cggtcggcat cgcgctcttt cccggagaat ataccatgcc
     2641 gtggggtgag atcgccgcgg cgtcgaccat cgcaaccatt ccgttaattc tgctcacttt
     2701 gatttttcaa cgcggcatcg tgcgcggctt gtctgctggt gcggtcaaag gttgatacct
     2761 atggccctgg aggatttatc gctggactct cagtaaagca aatttccaag cgcttcggcg
     2821 ccgcggaagt cttgcaagat gtttctttcg atgtcgccga cggtgcattt tgcatcctgg
     2881 tcggtccttc gggttgcggc aaatcgaccc tgttgcgttt gatcgccggc ctcgatcagc
     2941 cgagcggcgg tgaaattttc atcggcgccg agcgcgtcga cgccttggcc ccgcgcgacc
     3001 gggatgttgc cttcgtattt caaaactacg cgctttatcc gcatctgacc gtatttgaaa
     3061 atctcgcgtt ttcgctgcgc ctgcgcggcg tagcaaaaat cgaaatcggt cagaaggtca
     3121 aagaggccgc gcggctgttg gaaatcgagg acttgttgga acgccggcca caggcactga
     3181 gcggcggcca gagacagcga gtggcgctgg gcagggccat cgtccgccag ccgaggattt
     3241 ttctcttcga tgagccgctc tcgaatctcg acgcggcgct gcgcgccggc atgcgcgttg
     3301 agttggcgcg tttgcatcac cgcctgggaa ccacgatcgt ctatgtgacc cacgaccagg
     3361 cggaagccct aacacttggc gagcagatca ttgtcctgga ccgaggaata atccagcaga
     3421 tcggctctgc ggcatcggtg tatcatacac cggccaatgt catggttgcc ggttttgtcg
     3481 gcagcccgca aatgaatcta cttgcaggcg atgtcgacac cgacggcgcc gtgtttcagt
     3541 gcgcgaatct gcgcttggat ctcacccatg tgctgaaaaa atcacgcgcg caacgattac
     3601 cccggtcact gaccatcggt atccgcgccg aagatttcgc catcgccgaa ccggcggccg
     3661 catggatcac cggcgatata gatttaatcg aggatctcgg ttccgaccgc taccttcggg
     3721 tcaaatgcga ccgaatcgag ctgatcgtca gggctggcag agagctaaaa gttcagcgcg
     3781 gcgatcgcat cggcctcaac gtcagtgtcg atcgaatgca tttctttgca gacggcaaac
     3841 gctatgaact ttaaccttcc tacgatgcgc ctttgcagcg cagcggcccg ccaacattgg
     3901 catttgagtt ttgccggcaa tcgtattaac accaggcaaa ttagcgatca cggagggcgg
     3961 catatgaaaa tgatcaaccg agacctttca gcggacaagg acggcgtgcc cggttatctc
     4021 gcccatccag agcgcaaaga tcgcggcccc ggcgtgctca tcgtgcacca tcactacggc
     4081 gtcaccggca acatgaaggt caacgtctgc aacttcgcca agctcggcta cacaacggtc
     4141 gttcccgact tatataaaat gctcggcgaa tccgacggcg ttcacgatgc gcaaaaaaaa
     4201 accaccgacg gtcagttcat cgagatcctc aaccgctcct ggaattatct caccagcaga
     4261 ccggaggtgg acgccaagcg ctgtgccgtg gtcgggtact gcatgggcgg ccgcatcggc
     4321 attcacttcg tcgccgcaac gccgagcgtg cgcgccttcg tcggctacta tccatcggtg
     4381 cgcgatgaag agccgagcaa actccgaccg cgacatccca acgacgccgt caaagagttc
     4441 aattgccctt cgcaaatctt cctcggcggc aaggaccatg tcgccagcat ttcggtgcag
     4501 caaaaactga t
//
LOCUS       DASQKS010000074         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_462894, whole genome shotgun sequence.
ACCESSION   DASQKS010000074 DASQKS010000000
VERSION     DASQKS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462894"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2406
                     /gene="dnaE"
                     /locus_tag="VE170_01920"
     CDS             <1..2406
                     /gene="dnaE"
                     /locus_tag="VE170_01920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYJ14214.1"
                     /translation="GRGSSAGSLVAYAMRITDLDPIRHVLLFERFLNPERKSMPDIDV
                     DFCIRGRAQVIQYVKDKYGADRVAQIATFGTLKAKAAIKDVGRALGFSFAEADVIAKL
                     IPAPKQGFDFPLTEAIKMEPRLGELMKNDVRVKTLMDHALRLEGLVRHASTHAAGVVL
                     SNLPLVEHCPLFVDKEGGIVTQYEMTWVEKIGLVKFDFLGLKTLTLIHDCLKAIEAGR
                     GVKIDIDRLPLDDKKTYKTLCQGNTTGIFQLESTGIREMTVRIRPNCFEDLVAILALY
                     RPGPLDSGMAEDFINRKSGKEKIKYLHPWLETILKDTYGVIVYQEQVMQTAQILAGYS
                     MGDADLLRRAMGKKDPEEMAAQRERFVSGAVSKKIEEKRATEIFDQMETFARYGFNKS
                     HSAAYALVSYQTAYLKTHYPVEFMAALLTSEMGDTDKVIKNLADCRDKDIVVLAPDVN
                     ESGANFTPVGAKIRFGLAGVKNVGEKAVDVIIDSRTKDGPFESLFDFCRRVDLTAVNR
                     RVIESLIKCGAFDGTQVSRARMSGALDDAMKGGQAHQRDQASNQIDIFAMLGAPTRSG
                     RKAGETYPPATEWSPQEALVFEKEALGFYITGHPLDKYERVLKKISSGTVAALKERAQ
                     SGEVRLGGVVSGLRLRNTKKGDRYGSFNLEDKTGFIEVLIWPEAYKKCADLLSADDPI
                     YVKGKMEVGEERIQVIGNEVTLLAEAAKNPKNNVPSAVAEKVDLYVREDAVSAEELER
                     LRDMLLDYPGRHTVYLHLRAPAAGETVIELPEQVRIAPSRELENLVGERFGMRVSFHS
                     LNT"
     gene            2412..>3337
                     /gene="hflX"
                     /locus_tag="VE170_01925"
     CDS             2412..>3337
                     /gene="hflX"
                     /locus_tag="VE170_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYJ14215.1"
                     /translation="MISTREPQERAVLVGVGLPSRDHKLPIDYSLEELERLAETAGAL
                     VVHKYSQQVRSVTPATLIGRGKVDDICAGLQEHHANLVIVDEDLTPAQQRNLENAFKV
                     RVVDRSQLILDIFAKRARSNEGKLQVELAQLEYLMPRLTRQWTHLSRLGGGIGTRGPG
                     ETQLEVDRRRVRERIGHLKRRLTTVERTRSLQRRERDEVPFATVALCGYTNAGKSTLM
                     NLLTRAGVFVEDKLFATLDPTTRALRLPNGDKVMIVDTVGFINKIPHSLIEAFKSTLE
                     EVSRADLLLHLVDLANPLYEEQIQIIEKVLAE"
BASE COUNT          749 a          970 c          988 g          630 t
ORIGIN      
        1 ggacggggct cctcggccgg cagcttggtt gcctatgcca tgcgcatcac cgacctcgac
       61 ccgattcgtc acgtcttgct gttcgaacgt tttctcaatc ctgaacgcaa gtcgatgccg
      121 gatatcgatg tcgatttttg cattcgcggc cgggcccaag tgattcaata tgtcaaggac
      181 aaatacggcg ccgaccgggt ggcgcagatc gccaccttcg gtaccttaaa ggccaaagcg
      241 gcgatcaaag atgtcggccg cgccctgggc ttctccttcg ctgaagccga tgtcatcgct
      301 aaactgattc ccgcacccaa gcaaggcttc gattttccat tgaccgaagc gataaagatg
      361 gaaccgcggc tcggcgagct gatgaaaaac gacgtgcggg tgaaaacctt gatggaccat
      421 gcgctgcggt tggaaggact ggtgcgccac gcctcgaccc atgccgccgg cgtcgtcctg
      481 tccaatctgc cgttggtcga gcactgtccg ctgtttgtcg ataaagaagg gggtatcgtc
      541 acccagtatg aaatgacctg ggtggaaaag atcggcctgg tgaaattcga ctttctcggg
      601 ctcaagaccc tgacgttgat tcacgactgt ctgaaggcga tcgaggcagg acgcggcgtc
      661 aaaatcgata tcgaccgtct accgcttgac gacaagaaaa cttacaagac cctctgccag
      721 ggcaacacca ccgggatctt ccagctggaa agcaccggta tccgcgagat gaccgtgcgc
      781 atacggccta actgtttcga ggatctggtg gcgattctag cgctttaccg gcccggtccc
      841 ctcgatagcg gcatggccga agattttatc aaccgcaaaa gcggcaaaga aaaaatcaaa
      901 tatctccatc cgtggctcga aaccattctc aaagacacct acggcgtcat cgtctaccaa
      961 gaacaagtca tgcaaacggc gcaaatcctc gccggctact cgatgggcga cgccgacctg
     1021 ctgcgccgcg ccatgggaaa aaaagatccc gaagagatgg cggctcagcg cgagcgcttc
     1081 gtcagcggcg cggtgtccaa gaaaatcgaa gagaagcgcg ccaccgagat cttcgaccag
     1141 atggaaacct tcgcgcggta cggattcaat aaatcccact cggcggccta tgccctggtt
     1201 tcctatcaga ccgcctatct caaaacccat tacccggtgg agttcatggc cgcgctgttg
     1261 acctcggaaa tgggcgatac cgataaggtc atcaaaaatc ttgccgactg ccgcgacaaa
     1321 gatatcgtcg tgctcgcgcc cgacgtcaac gagagcggcg caaattttac tccggtgggg
     1381 gcaaagattc gtttcggctt ggccggtgtc aaaaatgtcg gtgagaaagc cgtcgacgtg
     1441 attatcgaca gccggaccaa agacggtccc tttgaatccc tgttcgattt ttgccggcgg
     1501 gtcgacttga ccgcggtgaa ccggcgcgtg atcgagagtt tgatcaagtg cggcgccttc
     1561 gacgggacac aggtatcgcg ggcgcgcatg agcggcgcat tggacgacgc catgaagggc
     1621 ggtcaagcgc accagcgcga ccaggcaagt aaccagatcg atattttcgc catgctcggt
     1681 gcgccgacca ggagcggtag gaaggccggg gaaacgtatc cgccggcgac cgaatggtcg
     1741 ccacaagagg cgctggtgtt cgagaaggaa gcgctgggat tctacatcac cggccatccc
     1801 ctcgacaaat acgagcgggt gctcaagaaa atcagcagcg gcaccgtcgc cgcgctcaag
     1861 gagcgcgccc agtccggcga ggtcaggctc ggcggtgtcg tctcggggtt gcgcctccgc
     1921 aacaccaaga agggcgatcg ctacggcagt ttcaacctcg aggataaaac cggcttcatc
     1981 gaagtgttaa tctggccgga agcgtataag aagtgcgccg acttgctcag cgccgacgac
     2041 ccgatctatg tcaagggcaa gatggaagtc ggcgaggaac ggattcaagt gatcggcaac
     2101 gaagtgacgt tattggccga agcggcgaag aatcccaaga ataatgttcc cagcgccgtc
     2161 gccgagaaag tcgatcttta tgtccgtgag gacgccgtgt ccgccgaaga attggaacgt
     2221 ttgcgcgata tgctgctgga ttatcccgga aggcacaccg tctatctgca cctgcgggcg
     2281 ccggccgccg gcgagaccgt catcgagctg cccgaacagg tgcgcatcgc gccgtcgcgc
     2341 gaactcgaaa acctggtcgg cgaacggttc ggcatgcggg ttagttttca ttccctaaac
     2401 acttgaagcc attgatttcc accagggagc cacaggaaag ggccgttctg gtcggcgtcg
     2461 gcttgccgtc gcgcgaccac aagctgccga tcgattatag tttggaagag cttgagcggc
     2521 tcgccgagac tgccggcgcg ttggtggtgc acaagtattc ccagcaagtg cgcagcgtca
     2581 cgccggcgac tttgattggc cgtggcaagg tcgatgatat ttgcgccggt ctgcaggagc
     2641 atcacgccaa cctcgtcatt gttgatgagg atctgacgcc ggcgcagcag cgcaatttgg
     2701 aaaacgcctt caaagtacgc gtcgtcgacc gcagccagtt gatcctcgac atcttcgcca
     2761 agcgcgcgcg cagcaacgaa ggcaagctgc aagtggagct ggcgcagttg gaatatctca
     2821 tgccgcgatt gacccggcag tggacccact tgtcacgcct gggcggcggc atcggcaccc
     2881 gcggaccggg cgaaacccaa ttggaagtcg accgccggcg cgtgcgcgag cgcatcggcc
     2941 acctgaaacg ccggctgacc accgtcgagc gcacgcgcag cctgcaacga cgcgagcgcg
     3001 atgaagtgcc cttcgccacg gtcgccctgt gcggctacac caacgccggc aagtcgacgt
     3061 tgatgaattt gctcacccgc gccggcgtgt ttgtcgagga taaattattc gcgactctgg
     3121 acccgacgac gcgagcgctg cgcctgccca acggcgataa agtcatgatc gtcgataccg
     3181 tcggcttcat taataagatc ccgcattcgt tgatcgaggc gttcaagagc acgctggaag
     3241 aggtgagccg ggccgatttg cttttgcatc tcgtcgacct ggccaatccg ctctacgaag
     3301 agcagattca gatcatcgaa aaggtcttgg ccgaaat
//
LOCUS       DASQKS010000075         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_883770, whole genome shotgun sequence.
ACCESSION   DASQKS010000075 DASQKS010000000
VERSION     DASQKS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883770"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VE170_01930"
     CDS             <1..177
                     /locus_tag="VE170_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYJ14216.1"
                     /translation="FVVNTKRPRKGRNPQTGDEIIIDGRKVLTFKASQIMKKSLNTMA
                     SSPAVSAPDAPGSE"
     gene            187..540
                     /locus_tag="VE170_01935"
     CDS             187..540
                     /locus_tag="VE170_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYJ14217.1"
                     /translation="MTPALPEKIYFKIGEVSEIVGVEPYVLRYWETEFDLLKPSKAPS
                     KHRLYKKRDVELLLDIKRLLYTEGFTIEGARKKLREVKKDEKDQLKLPLADRKYKNAL
                     IKVKKDLESLRKLLT"
     gene            654..730
                     /locus_tag="VE170_01940"
     tRNA            654..730
                     /locus_tag="VE170_01940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Pro,seq:ggg)
     gene            1242..1856
                     /locus_tag="VE170_01945"
     CDS             1242..1856
                     /locus_tag="VE170_01945"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYJ14218.1"
                     /translation="MVQEQIIARGVTDARVVAALRKIPRHVFVDPGIVNRAYDDCALP
                     IGEKQTLSQPYMAARMSEALALSGGEKVLEIGTGSGFQTALLAELCFNVFSVEKLRAL
                     SRRARALLDQLDYHNIALHVGDGTIGWSEHAPYDAIIVTAGAPQAPRPLLDQLAVGGR
                     LVIPIGDEQSQTLTRLVRTRTSFKKEQLGECKFVKLLGKYGWRE"
     gene            1904..2668
                     /locus_tag="VE170_01950"
     CDS             1904..2668
                     /locus_tag="VE170_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HYJ14219.1"
                     /translation="MACFMNFRLRWLWAGLALLALAGCVTFKSSPPAPPSRPGSNIFH
                     VVKPGENLFRIGKAYDVSIDELAKANRLKDANQIYVGQKLLIPGANRQLPVDTIAPLE
                     SVPVPRALPPPAEIGDDPLVWPVSGKINSNYGPRGSSFHDGIDIAAPEGTPILAVERG
                     EVIYSDQLRGYGNIVILRHQGGLVSVYAHNQVNLVHEGQQVIKGEVIAKVGSTGRVSG
                     PHLHLEIRKNNTAQDPLRYLPRLCCLSASDTVSPKD"
     gene            2679..3827
                     /locus_tag="VE170_01955"
     CDS             2679..3827
                     /locus_tag="VE170_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYJ14220.1"
                     /translation="MKELATVRNDVVGSLLRPPRLKTARQQYDEGGLTLEQLRRIEDQ
                     AIGAAVKLQEEVGLSVVTDGEYRRLNFQDSFGESVTGYDAGKPSLKLYEKRVEGGSPL
                     RRWEIPDQGEQKGTAVAQRRPVLAKLGLARNVPAEEFRFLKQITGKPGKVALIGPDRI
                     AQRFDWQHSTTVYSGLDEFVGDVVKVEREIIGGLVEAGCRYVQIDAPGYTAYVDPPSL
                     AAMRARGEDPQENFSRSLKADNAVLAGFEDITFGIHLCRGNQRSMWHREGTYDEIAER
                     LLNELHHDRFLFEYDTPRAGGFEPLRFLPKGKVIVLGLVSTKVAELESIDDLKRRIDA
                     AAKYAPLEQIAVSPQCGFSSDVVGNLISDDDQKRKLALVVETARQVWG"
     gene            3914..4438
                     /locus_tag="VE170_01960"
     CDS             3914..4438
                     /locus_tag="VE170_01960"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HYJ14221.1"
                     /translation="MASIRGAIRDIPDFPKPGVVFKDITPLLSDGRLFAKTIDLIAAR
                     YEGQKVDTVLGIESRGFIFGSALAYKLGAGFSVVRKPGKLPYNTHSASYELEYGKDTL
                     EIHVDAIAPNARVVIADDLIATGGTAAATAALVSRLGAIVVECAFVIELRFLNGRERL
                     NPYPVYSVLQYDSE"
     gene            4588..5157
                     /locus_tag="VE170_01965"
     CDS             4588..5157
                     /locus_tag="VE170_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HYJ14222.1"
                     /translation="MKRIEQMSLWERLYFPEVFRGLMVTGYRFWRNLAVHTLQIFGLA
                     KNASASITVQYPEEKWLYPDTYRGRHRLTLKMDDTVQCTACYLCATACPAECIYIEAG
                     EYADKSVEKFPIRYEIDTLRCVYCGYCVEACPCDAIRMDTGTHPGNLGFSRKDFVETK
                     EVLMQRSKDLADKGKVGLYEEYVKPYRHV"
     gene            5195..>6220
                     /locus_tag="VE170_01970"
     CDS             5195..>6220
                     /locus_tag="VE170_01970"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HYJ14223.1"
                     /translation="MANRQTPNGTKPTGLTTQPLPASHKVYVHSHQRPEISVAMRAVM
                     LSEASNGHGKVSGNAHADHANAPHANAPHAKAPLMIYDTSGPYTDPEAQTDIRQGLKP
                     MRLDWIRGRGDVEELAGSSYHAPIKKNGKASDPQTERFPDSARRPILRAKAGCNVSQM
                     HYARKGIVTPEMEYVAIRENLGRQQAFESNGSGRTHAGYSFGAAIPKFVTPEFVRDEV
                     ARGRAIIPSNINHPESEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMIWATRWGGD
                     TVMDLSTGMDIHETREWILRNSPVPIGTVPIYQALEKVDGKAEDLTWEIFRDTLIEQA
                     EQGVSYFT"
BASE COUNT         1389 a         1734 c         1793 g         1304 t
ORIGIN      
        1 tttgtcgtca acaccaaacg tccgcgtaaa ggtagaaatc cccaaaccgg agatgaaatc
       61 ataatcgacg gccgcaaagt tttgaccttc aaagccagcc agatcatgaa aaaaagcttg
      121 aacacgatgg cgtcttcgcc ggcggtgtct gctcccgatg cgccagggtc tgagtaggcc
      181 aagttcatga cgcctgcgtt accggagaag atctatttta agatcggcga ggtgagtgag
      241 atcgtcggcg tcgagcccta cgtgttgcgt tactgggaaa ccgagttcga cctgctcaag
      301 ccgtctaagg caccgtccaa acaccggctc tataaaaagc gcgatgtcga gctcctcctc
      361 gatatcaaac gattgctcta caccgagggc ttcactatcg aaggcgcgcg caagaaactc
      421 agagaagtga aaaaggacga gaaagatcag cttaaactgc cgcttgccga tcggaagtat
      481 aaaaacgcat tgatcaaagt caaaaaagat ctcgagtctc tgcgcaaact gctaacctaa
      541 aattttctgt ggcgaatcgt tggcgccact gatcatttcc cttgcccacc gcttccgatg
      601 aaattgattt agcccaatca gagtgctaaa ttccggctgc aataaatcct cgtcggggcg
      661 tggcgcagcc tggtagcgcg cacgcttggg gtgcgtgagg tcgccggttc aaatccggcc
      721 gccccgacca ttcccgataa ttgctcggga tgaatatttt gccgcgggcg cgatgcggtt
      781 aatgtcccgg caatgaatgg gcgccagatc gacggccaac tgccggcaaa attattcgtc
      841 gagcattgtt gtaaagactg catgggatcg ctcacgccct tcagcctggc ggcgatccga
      901 gcgccccgac catgtcaccg ggcatgtagg cggctgaaac acttctccgg tctgcgccag
      961 ctaaaggatt tgcgacaaac tctcacaaaa cagctgatta aacccgttag cgcgccaata
     1021 aactagaaaa tcgccccgcg cctgtgctag attcctgaat tgaataaatg cagcttgagc
     1081 ttttttacag tatcgagtta accgacgatc cagcgcattt ctatcaggcg aatgttgcag
     1141 tgcagcagcg tcggtttgtg gcataccgga aacccttgat cccaacctaa gtgaagaatc
     1201 ccgaaccgat cgcgcatgat ttcgcatggg cccgcgaccg catggtccag gagcaaatta
     1261 tcgcccgtgg cgtgaccgac gcccgcgtgg tcgctgcttt gcgtaagatc ccgcgccatg
     1321 tcttcgtcga tccgggaatc gtcaatcgcg cctatgacga ttgtgccttg ccgatcggcg
     1381 agaaacagac tctgtcgcag ccctacatgg cggcgcgcat gagcgaggcg ttggctctgt
     1441 ccggaggcga aaaagtgctc gaaatcggca ccggatcggg ttttcaaacc gcgctcctgg
     1501 ccgagctttg cttcaatgtt ttttccgtcg aaaagttgcg tgcgctgtcg cgccgggcac
     1561 gcgcgctatt ggatcagctc gactatcaca acatcgcgct ccatgtcggc gatggcacga
     1621 tcggttggtc cgagcatgcg ccatacgacg cgattatcgt gacagccgga gcgccgcaag
     1681 cgccccggcc gctgctcgat caactcgccg tcggcggccg gttggtgatt ccgatcggcg
     1741 atgagcagtc ccagaccctg acccgtttgg ttcgaacccg tacgagtttc aaaaaagagc
     1801 agctcggcga atgcaagttc gtcaagctgt tgggcaagta cggttggcgt gagtagcgcc
     1861 gttcggttat ttcgttaggt ttgccagcgg cggcagtgtg tccatggcat gcttcatgaa
     1921 ttttcggtta cgatggctct gggcgggcct ggccttgctc gctctggccg gttgcgtgac
     1981 gttcaagagt agcccgccgg caccgccgtc gagaccggga tcaaatatat ttcatgtggt
     2041 caaaccgggt gagaatcttt tccgcatcgg caaagcgtac gatgtttcca tcgacgagtt
     2101 ggccaaggcc aatcggctga aggatgccaa tcaaatctac gtcggccaaa aactcttgat
     2161 ccccggtgcc aaccggcaac taccggtgga taccatcgct ccactggagt cggtgccggt
     2221 gccgcgcgcg ctgccgccgc ccgccgaaat cggcgacgac ccgttagttt ggccggtgag
     2281 tggcaagatc aattccaact acggcccgcg tggctcaagt tttcacgacg gcatcgatat
     2341 cgccgctccg gaagggactc cgattcttgc ggtcgagcgc ggcgaagtta tttatagcga
     2401 tcagctgcgc ggctacggca acatcgtcat cctgcgccat cagggcgggt tggtttcggt
     2461 gtacgcccat aaccaagtca atctcgtgca cgaagggcag caggtgatta agggtgaggt
     2521 gattgccaag gtcggcagca cgggacgagt cagcgggccc catctgcatt tggaaattcg
     2581 caagaataat acggcacaag acccgctgcg ctacctgccg cggttgtgct gtttatcggc
     2641 atccgatact gtgtcgccga aagattagag gttccatcat gaaagagttg gcgacagtta
     2701 gaaacgatgt ggtcggtagc ctgctgcggc caccgcgact taaaaccgcg cggcagcaat
     2761 acgacgaagg cgggttaaca ctcgagcagt tgcgccggat cgaagatcag gcgattggcg
     2821 cggcggtcaa gctgcaagaa gaagtcggcc tgtcggtagt caccgacggc gaataccgcc
     2881 ggctcaattt tcaagatagt tttggcgagt cggtgaccgg ctatgatgcc ggcaagccca
     2941 gcctaaaact ttacgaaaag cgggtggagg gtggcagtcc gctgcgccgt tgggagatcc
     3001 ctgaccaggg cgaacagaag ggcaccgccg tggcacagcg tcggccggtg ctggccaagc
     3061 tcggattggc gcgcaatgtg ccggcggaag aatttcgctt cttaaagcaa atcaccggca
     3121 agccgggcaa ggtcgctttg atcggcccgg accggatcgc gcagcgattc gattggcagc
     3181 attctacaac tgtctattcg ggcctcgatg agttcgttgg cgacgtcgtc aaagtagagc
     3241 gcgaaatcat cggtggcttg gtcgaagccg gttgccgcta cgtccagatc gacgcgcctg
     3301 gctatacggc ctatgtcgat ccaccatcgc tcgccgccat gcgcgcgcgc ggcgaagacc
     3361 cgcaggagaa tttcagccgc tcgctaaaag ccgataacgc cgtgctcgcc ggcttcgagg
     3421 atattacatt cggcattcac ctctgccggg gcaatcagcg cagcatgtgg caccgcgaag
     3481 gcacctacga cgaaatcgcc gagcggctgc tcaatgagtt gcatcatgac cggtttcttt
     3541 ttgaatacga cacgccgcgc gccggcggct tcgagccctt gcgcttttta cccaagggta
     3601 aggtcatcgt tttgggcttg gtgagcacca aggtggccga gcttgagagc atcgacgatc
     3661 tcaaacggcg catcgatgct gcggccaagt acgcgcctct cgaacagatc gcggttagtc
     3721 cgcagtgcgg cttttcctcc gatgtcgtcg gtaatctaat tagcgacgac gatcagaagc
     3781 gcaagctcgc attggtggtg gagaccgcac ggcaggtttg gggctagccc tcgcagcacg
     3841 agggcccggc aactttcaga aatcaaattc gaaattctag agtttcgaaa ggtggctcgg
     3901 tatgggtgac gacattgcca gtatccgcgg cgcgatccgc gacatccctg attttcccaa
     3961 gcccggagtt gtcttcaagg acatcacgcc gcttctaagc gacgggcgat tattcgctaa
     4021 aaccatcgac ttgatcgcgg cacgctatga ggggcaaaag gtcgacacgg tgttgggcat
     4081 cgagtcccgt ggatttatct tcggttccgc gctggcgtat aagttgggcg ccggatttag
     4141 cgtcgtgcgc aagcccggca aattgcccta taacacccat agcgccagct acgaactcga
     4201 atacggtaaa gatacgctgg agatccatgt cgacgccatc gcgccgaacg cccgggtggt
     4261 gatcgccgac gatttgatcg ccaccggtgg caccgccgcg gcgaccgctg cgctagtttc
     4321 acgcctgggc gcgatcgtgg tcgagtgtgc cttcgtgatc gaactgaggt ttctcaacgg
     4381 ccgcgaaaga ctcaatcctt atccggtata ttcggtgctc caatacgatt cggagtaacg
     4441 gcgcaatagc tccgttttcg tgacgtttct attccttgac ttcatccgcc atcgtctata
     4501 acttgagatc aacttgaccg actaacctag tgaaaagttt aggcttcgtt gctagatgcc
     4561 gacgctgtag caaattagga tcgatggatg aaacgcattg aacagatgtc gctgtgggaa
     4621 cggctgtatt tccctgaagt ctttcgcggc ttgatggtga ccggctatcg tttctggcgc
     4681 aatctagcgg ttcatacgct gcaaatattc gggctcgcga agaatgccag cgcatcgatc
     4741 acggtccaat atcctgaaga gaagtggctg tatcccgata cctatcgcgg ccgccaccgg
     4801 ctcactttga aaatggacga taccgtgcaa tgcacggctt gttatctctg cgccaccgcc
     4861 tgcccggcag agtgcatcta catagaagcc ggtgagtatg ctgacaagtc ggtggagaaa
     4921 tttcccatac gttacgagat cgataccttg cgctgcgtct attgtggtta ctgcgtcgaa
     4981 gcctgcccct gcgatgcgat ccgcatggat acgggaaccc atccgggcaa tttgggtttt
     5041 agccgcaaag atttcgtcga gaccaaagaa gtgctgatgc aacgatcgaa ggatttggcc
     5101 gacaagggca aagtcgggct ttacgaagaa tacgtcaaac cctatcgcca cgtctgacca
     5161 cggcggcgat ttcccagccg aaggaggcaa catcatggca aataggcaaa ccccgaatgg
     5221 caccaagccc actgggctta ctacgcagcc gctaccggca tcgcacaaag tttacgtcca
     5281 tagtcatcag cgtcctgaga tcagcgtggc gatgcgcgcc gtcatgttgt ccgaagccag
     5341 caatggccat ggcaaggtta gcggcaacgc ccacgccgat catgccaatg cgccacatgc
     5401 caatgcgcca cacgccaaag cgccattgat gatctacgac acctctgggc cttacaccga
     5461 tccagaggcg cagaccgaca tccgccaggg tttgaagccg atgcgtctcg attggatccg
     5521 cgggcgcggc gatgtcgaag aactggcagg ttcgagctat cacgcgccga ttaagaaaaa
     5581 cggcaaggcg agtgatccgc agacggaacg gtttcccgac agtgcgcggc ggccgatcct
     5641 gcgcgctaag gcgggttgca atgtttcgca aatgcactac gcccggaagg gcattgtcac
     5701 gcctgagatg gagtacgtcg cgattcggga aaatctcgga cggcagcaag cttttgagag
     5761 caacggtagc ggtcgcaccc atgccggtta tagtttcgga gcggcgatcc ccaagttcgt
     5821 cacgccggaa ttcgtgcgcg acgaagtcgc ccgcggccgc gcgatcattc cgtccaatat
     5881 caatcatccg gagagcgagc cgatgatcat cggccgcaac tttttggtca agattaacgc
     5941 taacatcggc aactcggcag tggcgtcatc catcgaagag gaagtcgaaa aaatgatttg
     6001 ggcgactcga tggggcggcg acacggtgat ggatctttcc accggcatgg atattcacga
     6061 gacgcgcgag tggattttgc gcaattcacc ggtgcccatc ggcacggtgc cgatctacca
     6121 ggctttggaa aaggttgacg gcaaggccga ggatctcact tgggaaattt ttcgcgacac
     6181 cttgatcgag caggcagagc agggcgtgtc ttattttacc
//
LOCUS       DASQKS010000076         7557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5502360, whole genome shotgun sequence.
ACCESSION   DASQKS010000076 DASQKS010000000
VERSION     DASQKS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5502360"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /gene="xseA"
                     /locus_tag="VE170_01975"
     CDS             <1..564
                     /gene="xseA"
                     /locus_tag="VE170_01975"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HYJ14224.1"
                     /translation="WAFNEEAVARAIFASQVPVISAVGHEIDFTIADFVADARAPTPT
                     AAAQMVVPCKRDLVEQVEYAAARLANGVDFKIEQLREIFQGLVKRLSDPSRKLRENQQ
                     RVDELSIDLTRRLRQRLRQLKDRLAERSGRLGALSPLAVLERGYSITHKLPEERIVKS
                     AAELQVGDALRIRFAYGKALCRVEEKE"
     gene            566..1486
                     /locus_tag="VE170_01980"
     CDS             566..1486
                     /locus_tag="VE170_01980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYJ14225.1"
                     /translation="MTVRPFCAFLWLMILAVPSSFAADAVKIHWPKPLEASQGDLFEV
                     SVGGEDLLEVDGLLGKEKVYFYPTDKGVYAAVVGVDVEAKPATVKLMLKATTAAGERR
                     QREIPVKIRAKAFHQESFNVAPSFDQMNPETLTEIRREQQAFARVFAAPAAERFWNLP
                     FVRPVSEEASASSFGSRRIINGVPRAPHTGTDLSAPASTEVVASNHGRVVLVGNFYFA
                     GGSVVIDHGGSLYSMYFHLAEFKVEEGAMVRRGDVIALSGASGRVTGPHLHWGVRLNN
                     ARVDPLDLLRKFSGLMDSTDSRKAAAKAEK"
     gene            1488..1745
                     /locus_tag="VE170_01985"
     CDS             1488..1745
                     /locus_tag="VE170_01985"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HYJ14226.1"
                     /translation="MAKKETPNKNFEGALEELEQVVELLESGELPLDDSLAAFEKGVG
                     LVKFCNQKLNEVEKKVELLVKDKAGNLQLKSLADLPEEDTD"
     gene            complement(1771..2484)
                     /locus_tag="VE170_01990"
     CDS             complement(1771..2484)
                     /locus_tag="VE170_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ14227.1"
                     /translation="MNAIADFAIDQAMHIIARILTGNRLRTVARGLENVPPDGPAIIA
                     ARHYHHLFDGLAFFATVERRFHFVVTADWAQSRRSKFLMGTLNRLARWPMVLREDALR
                     NGATHQRTLFEPRDLRRYHLAALRQSAQLLKEKRLLIMFPEGYPNIDPNYTPKTSADE
                     FLPFKSGFAVIAETAERITNESIPIIPAGIAYQIGATWLAQLNFGPAVYRRDFSSRRQ
                     LTEHVADAVKQLCAVQKNI"
     gene            complement(2509..3663)
                     /locus_tag="VE170_01995"
     CDS             complement(2509..3663)
                     /locus_tag="VE170_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ14228.1"
                     /translation="MKPCYSPANRKTGNGKERQSLFGLSRTKLHINLATVPSHERVTV
                     LLPVLNEAGRIDACLAALCQQPEEVQEVLVIDGGSKDGTQGIVQRHRAVDARVRLIDA
                     SPVDPRWTGKVWGLQRGLLSSSPKSDWILCVDADVKVQPQLVRSLLHHAQRSAVSIFS
                     VATAQRLSGNLDALIHPAMLTTLVYRFGSPGTATSNRHRVQANGQCFFARREILLRTE
                     AFRTAQGSLCEDITIVRRLAECGEQIGFYESDGLVEVKMYGSWRETWNNWPRSLPMRD
                     QYFGWRESIGLVGVLVFQALPMPTLLIGALIRLPLALLILPGILSIIRLGVHFGVARA
                     YPNRPWAYWLSPIADLPVVLRIIQCALRRRHRWRGRIYRRGKGGVFEPVG"
     gene            complement(3632..4336)
                     /locus_tag="VE170_02000"
     CDS             complement(3632..4336)
                     /locus_tag="VE170_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14229.1"
                     /translation="MLANSIHAQEEPLLVPGTYRLEMIMASTTRVPVFGTSKSASKSV
                     SLVEIKRDGTALIQSHKTCDFRVLDDSKFIKMIFPEKFIGALAKHSYPLQLEKDAQGW
                     RYRADLGVERIGYKSTANETGLPDKIDDPAVYDWDGDGKPAATIKISIPLLPNGEFYI
                     VQRGHSILNGRIVQPGRIEGSIETPLFEQRVLGAWPGFLNQSAEVEPDPKESRFILSL
                     APGGSSCETLLFAGKS"
     gene            4713..6104
                     /locus_tag="VE170_02005"
     CDS             4713..6104
                     /locus_tag="VE170_02005"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HYJ14230.1"
                     /translation="MKTLVAILSDPRLTVRRAGEPDPDGRCVVYWMQRAQRAVDNPAL
                     TAAINAGNCLGKPVVVFFQLVPHAHHANLRHYQFLVEGLAEIAAALQKRKVGFVLRRY
                     PDHGLLRFCSEVRPCLVIADENPLKEAERTKTSIAEKIVLPFWTVDSDVVVPAKLLTK
                     EHYAARTIRPKIRALLPQFMGKIENPVAKVRWRNPTHVLSLVSDPALLDNFPIDRGVA
                     PSAYFHGGSNEAKRRLQRFLRSRLGGYATRRNKPDLDGTSQLSPYLHFGQIGVQAVAL
                     AVQQADAPAQDRSAFLEELIVRRELAVNFVRFNRDYENFDACEPWADVTHRKHSRDPR
                     LHLYSEKQLEDAETHDPLWNAAQMQMVLTGWMHGYLRMYWAKKILEWTESPQAAYQIA
                     VRLNDRYELDGRDPNGYAGIAWAIVGKHDRAWGPERPIYGKIRYMSFASTSRKFDSSG
                     YIGRIAQLEKARS"
     gene            complement(6120..6587)
                     /locus_tag="VE170_02010"
     CDS             complement(6120..6587)
                     /locus_tag="VE170_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ14231.1"
                     /translation="MKSYWLRKIIWLPQSPAEIFDFFSNPGNLDRLTPAWLNFEIVTP
                     ATSQIKEGALLDYRLRLRGMPIRWQSKIAVWEPPHRFVDRQVRGPYSRWIHEHIFEER
                     ANGTLVGDNVEYAVYGGTIVQKFLVAPDLERIFAYRHKILQELFNPAKLTPAQ"
     gene            complement(6622..>7557)
                     /locus_tag="VE170_02015"
     CDS             complement(6622..>7557)
                     /locus_tag="VE170_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HYJ14232.1"
                     /translation="KVAISACLLGHQVRFDGGHKRDLFLTELFGKFVDLVPICPEVEV
                     GMGVPRETVRLVGAPSHPRMIAEKSGKDWTDAMHRFAAKRVRDITEKHLSGYVLKKNS
                     PSCGMARVKVYNSKGMPERQGRGLFAAALMSQWPLLPVEEEGRLNDLKLRENFIERVF
                     AYHRWLRATSARRSPGALVRFHTSHKFLLLAHSERHYRQLGRLVAAAKQKSLPALYDD
                     YGRFFMEALAVHATEKTHANVLDHMMGYFSEQLTAGERQELVQLIDEFRRRLIPLIAP
                     ITLMRHYIEKYQVEYLQGQRYLEPNPKELALRNHI"
BASE COUNT         1652 a         2030 c         2198 g         1677 t
ORIGIN      
        1 tgggcgttca atgaagaagc ggtggcgcgg gcgatcttcg cctcgcaggt tccggtgatt
       61 tcggcagtgg gtcacgagat tgattttacc atcgccgatt tcgttgccga cgcccgcgca
      121 ccgacgccca cggcggcggc ccagatggtc gtgccatgca agcgcgatct ggttgagcaa
      181 gtcgagtatg ccgcagcacg tttagcaaac ggggtcgatt tcaaaatcga gcagttgcgt
      241 gaaatatttc agggcttggt taagcggttg tcggatccga gccggaagct gcgcgagaat
      301 cagcaacgtg tcgatgagtt gtccatcgat ctaactcgcc ggctgcgaca gaggttgcgc
      361 cagctcaaag accgattagc cgagcgctcc ggtcgtttgg gcgcgctcag cccactggcg
      421 gtgctcgagc gcggctatag cattacgcac aagttgccgg aggagcggat cgtcaagagt
      481 gcggcggaac ttcaagtcgg cgatgcgctg cgcatccgtt tcgcctatgg gaaagcgctg
      541 tgccgagtgg aggagaagga atgagatgac agtgcgccct ttctgcgcct ttctctggct
      601 catgattctc gcggtgccgt cgtcgttcgc tgccgacgcg gtgaagattc actggcccaa
      661 gcccttggag gcgtctcagg gcgacttgtt tgaagtttcg gtgggcggtg aagacttact
      721 tgaggtggac ggactgctgg gaaaagaaaa ggtttatttt taccccaccg ataagggggt
      781 gtatgcagct gtagtcggcg tcgacgtcga agccaagccg gcgacggtta aattaatgtt
      841 gaaggcgact accgccgccg gtgaacggcg tcaaagggaa atcccggtta aaataagagc
      901 caaggctttt caccaggaat ccttcaacgt cgcgccgagc ttcgaccaaa tgaacccgga
      961 gactctcact gagattcgcc gcgagcagca agcctttgcc cgcgtgttcg cggcccccgc
     1021 ggcggagcgt ttctggaatc tgccgttcgt gcggccggta tccgaagaag cgtcggcgtc
     1081 ttccttcggt tcacggcgta tcatcaatgg cgtgccgcgc gccccccaca cgggcaccga
     1141 tctgtcggcg ccagccagca ctgaagtggt tgccagtaat catggccggg tggttttagt
     1201 gggcaatttt tacttcgccg gcggcagtgt cgtcattgat catggcggta gtttatactc
     1261 gatgtatttt cacctggcgg agttcaaggt cgaagaaggt gcgatggtac gccgtggcga
     1321 tgtgattgcg ctctcgggtg ccagcgggcg ggtcacgggg ccgcatttgc actggggcgt
     1381 gcgtttgaat aacgcccgcg tcgatccgct ggatctactg cgtaaatttt ccggtctaat
     1441 ggattctacc gactcccgca aagcagcggc aaaagcggag aagtgacatg gcaaaaaaag
     1501 aaacccccaa caaaaatttt gaaggtgctt tggaagagtt ggaacaggtc gtcgaattgc
     1561 tcgaatccgg cgaactgccc ctcgacgatt ctctggccgc cttcgaaaaa ggcgtcggcc
     1621 tggtaaagtt ttgtaatcag aaactcaatg aagtggaaaa aaaggtcgaa cttctggtca
     1681 aggacaaagc gggcaacctg cagctcaagt cgcttgctga cctgcccgaa gaagacaccg
     1741 attaattttg cgcagccaat tgatgttaca ctaaatgttt ttctgtactg cgcaaagctg
     1801 tttcacggca tcggcgacat gttcagtcaa ctgccggcgc gatgagaaat cgcgccgata
     1861 gaccgctggg ccgaaattca attgcgccag ccaggttgcg ccgatttgat aggctatccc
     1921 cgcgggaatg atcggaatgg attcgtttgt gatgcgttcg gcggtttcgg caatgacggc
     1981 aaagccagac ttaaacggta gaaactcatc ggcgctagtc tttggcgtgt agttgggatc
     2041 gatgttcgga taaccctcgg gaaacataat cagcagacgt ttttccttga gaagttgggc
     2101 cgattggcgc aaagccgcca gatggtagcg ccgcaaatcc ctgggctcga agagcgttcg
     2161 ctggtgtgtc gcgccgtttc gcagcgcgtc ttcgcgcagc accatcggcc agcgggcgag
     2221 gcgattgagc gtgcccatta aaaatttgct acgccggctc tgcgcccaat cggccgtcac
     2281 gacgaaatga aaacgcctct ccaccgtggc aaaaaaagcc aggccgtcga acaggtggtg
     2341 gtaatgccgc gcggcgatga tcgccggccc atcgggtggt acattctcta acccccgcgc
     2401 aacggttcgc aagcgattac cggtaaggat gcgggcgatg atgtgcatcg cttgatcgat
     2461 ggcaaagtca gcgattgcgt tcactggacg tcggcgtggt tggttgatct atccgaccgg
     2521 ttcgaagacg ccacccttgc cgcggcgata gatgcggcca cgccagcgat gacgccgccg
     2581 cagcgcgcat tgaatgatgc gcaggacaac gggtaaatcg gctatgggcg atagccagta
     2641 agcccagggc cggttggggt aggcgcgggc gacgccgaaa tgaacgccca gtcggatgat
     2701 cgacagtatt cccggcaaaa ttagcagcgc caatggcaat cgaatcagcg cgccgataag
     2761 gagcgtcggc atgggcaagg cttgaaacac cagcacccca actaatccaa tcgattcgcg
     2821 ccagccgaaa tactgatcgc gcatgggcag ggagcgcggc caattattcc aggtctcacg
     2881 ccagctgccg tacatcttaa cttcgacgag tccatcggat tcgtaaaagc cgatctgctc
     2941 accgcattcg gcgagtcgcc gcacgatggt gatgtcttcg caaagcgagc cttgcgccgt
     3001 ccgaaatgct tccgtgcgta gtagtatctc gcgacgcgca aaaaaacatt gtccgttggc
     3061 ttgaacgcga tggcgattgc tcgttgccgt gccgggcgaa ccgaagcggt agactaatgt
     3121 ggtcaacatc gccggatgaa tgagcgcgtc gagattgccc gacaggcgct gtgcggtcgc
     3181 gacagaaaaa atactgaccg cgcttcgttg cgcatggtgc agcagcgagc gcaccagctg
     3241 cggttggact ttgacgtccg cgtcaacgca aagtatccaa tcgctcttag gtgaagaact
     3301 cagcagtccg cgctgcaatc cccagacttt tccagtccag cgcgggtcca cgggactggc
     3361 gtcgatcaat cgtacacgtg catcaacggc acgatggcgc tgcacgatcc cttgagtgcc
     3421 gtctttggac ccaccgtcga taaccaaaac ttcttgtact tcttcaggct gttggcacaa
     3481 agcggcgagg caagcgtcga ttcggccggc ctcgttcaat accggtaaca gcaccgtgac
     3541 acgttcatgg gaaggaaccg tggcaaggtt gatgtgcagt ttcgttctag ataggccgaa
     3601 caaggattgc cgttctttcc cgttgccggt cttacgattt gccggcgaat agcaaggttt
     3661 cacagctgct cccaccaggc gccaaactca aaatgaagcg gctctccttc gggtccggct
     3721 cgacctcggc cgattgattc aagaagcccg gccaggcgcc gagcactcgt tgttcaaaca
     3781 agggggtctc gatggagcct tcgattcttc ccggttggac gatgcgtcca ttgaggatcg
     3841 aatggccgcg ctgcacgata tagaattctc cattgggtaa cagcggtatt gagatcttga
     3901 ttgtcgcggc cggcttgccg tcgccgtccc aatcgtaaac cgcgggatcg tcgattttat
     3961 cgggcagacc ggtttcattg gcggtcgatt tgtagccgat ccgttcaacc ccgaggtcgg
     4021 cgcgatagcg ccaaccttgc gcatcttttt cgagttgtag aggatagcta tgttttgcca
     4081 aggctccgat aaacttttcg ggaaagatca ttttaatgaa cttggaatcg tcgagaacgc
     4141 gaaagtcgca ggtcttgtgg gattggatca gcgctgtgcc gtcgcgcttg atctcgacga
     4201 gggataccga tttcgatgcc gacttcgatg tgccgaagac cggcactcgg gttgtcgacg
     4261 ccatgatcat ttccaggcgg taggtgccgg gaacaagaag cggctcttcc tgcgcgtgga
     4321 ttgagttggc taacaacccg caaccaacga caagagcaga gactgcgcgc gttagaaatg
     4381 tcgtccgatt agggcgatta ttaaagcaat ttttgatcaa gtcaaaatta ggctggtgca
     4441 tttattgatg ccaaaattga aagatgaggc gtgacttagc gcagagcttg ttgtcgataa
     4501 aactatatcg cttagcgaac gcttcttctc acatctagca gtttttgtta ctgactacac
     4561 taggcgacac agacgagtct atcattgcct gggtttcggc gtctgaccag tcccggagat
     4621 tgcgttgcgt gcttctaagc tggaccgcgc ctaaacttgg gttaccggcg agtcattgcc
     4681 gcggtatgcc acaacgacaa agccaaagca gaatgaaaac actagttgct attctcagcg
     4741 atcccaggct aacagttcgc cgtgccggcg agcctgaccc ggacgggaga tgcgtagttt
     4801 attggatgca gcgcgcccaa agagcggtgg acaatccggc gcttacggcc gccattaacg
     4861 caggcaattg tctgggcaag ccggtggtgg tgttttttca actggttccg cacgcgcatc
     4921 acgccaatct ccgccactat caatttcttg ttgaagggct cgcagagatc gccgcggcgc
     4981 tgcaaaaacg aaaagtcggc ttcgttctgc ggcggtaccc cgatcatgga ttattgcgct
     5041 tttgttccga agtgcggcct tgtttggtca tcgcagatga aaatccattg aaggaagcag
     5101 agcgtaccaa aactagcatc gccgagaaaa ttgtcctgcc gttttggacg gtggattcgg
     5161 acgtggttgt gccggcaaag ctgcttacca aagagcacta cgcggcgcgg acgatccgtc
     5221 cgaagatccg cgccttgttg ccgcaattta tgggaaaaat agaaaatccg gttgcgaagg
     5281 ttcgatggcg caacccaacc cacgtgttgt ctctggtctc tgatccagcg ctgctcgata
     5341 atttccccat cgaccgtggc gttgcgccgt cggcctattt ccatggcggc tccaacgaag
     5401 ccaagcgccg tctccaacgg tttcttcgat cccggcttgg aggttacgcc acccggcgta
     5461 acaaacccga tctcgatggc acgagccagt tgtcgccata tcttcatttc ggccaaatcg
     5521 gcgtgcaagc agtagcgctc gcggtgcaac aggcggatgc cccagctcaa gaccgcagtg
     5581 catttttaga ggagctgatc gtccgccgcg aattggccgt taatttcgtt cgcttcaacc
     5641 gcgactacga gaacttcgat gcgtgcgaac cctgggcgga tgtcacgcac cgcaaacata
     5701 gtcgggaccc taggctgcat ctttacagtg aaaaacagtt ggaagacgcc gaaacccatg
     5761 atcccctatg gaatgctgcg caaatgcaga tggtgctgac cggttggatg cacggttact
     5821 tgcgcatgta ttgggccaag aagattcttg aatggacgga atcgccccag gccgcctacc
     5881 agatcgccgt tcggctcaac gaccgctacg agctggacgg gcgcgatccc aatggatatg
     5941 ccggtatcgc ctgggcgatc gtcggcaagc atgaccgggc ttgggggcca gaaaggccaa
     6001 tttacggaaa aatccgttac atgtcattcg ccagtacatc gcgtaagttc gacagctcag
     6061 gttatatcgg tcgcattgca caattagaaa aagcccgctc ttaggactta ggcgccagtt
     6121 cactgtgctg gcgtgagctt cgccggattg aacagttctt gtagaatttt gtgacgatag
     6181 gcaaaaatcc gctcgagatc gggtgcgact aagaatttct gtacgatggt accgccgtag
     6241 acggcgtatt cgacattgtc gccgactaaa gtgccgttgg cgcgttcctc gaagatgtgt
     6301 tcgtgaatcc accgcgagta agggcctctg acttgccggt cgacaaaccg gtgcggcggc
     6361 tcccagacgg caatcttgct ttgccagcga atcggcatgc cgcgcaggcg cagccggtag
     6421 tctaacagcg cgccctcctt aatttggctc gtcgccggtg tcacgatctc gaagttgagc
     6481 catgccggcg tgagacgatc aagattgccg gggtttgaaa agaagtcgaa tatttcggcg
     6541 ggtgattgcg gtagccaaat aatttttctg agccagtaac tcttcatgat agttgtttac
     6601 ccctgacgca atagcgatag cttagatatg gtttcgcagc gccagctctt taggattggg
     6661 ttccagatag cgctgtcctt gcagatattc aacttggtat ttctcaatgt agtggcgcat
     6721 caatgtgatc ggcgcgatca aaggaatcag ccggcgtcga aattcatcga ttaactgtac
     6781 cagttcttgt cgctcgccgg cggtcaactg ctcggagaag tagcccatca tgtgatccag
     6841 aacattggcg tgggtcttct cagtcgcgtg aacggctaga gcctccataa aaaaccggcc
     6901 gtagtcatcg tataacgcgg gaagcgattt ttgttttgct gccgccacca gacggccgag
     6961 ttggcggtag tgacgctcgc tgtgcgccag caataaaaat ttatgactgg tgtgaaagcg
     7021 caccagcgcc cccggtgacc ttcgcgccga agtcgctcgc agccagcgat gataggcgaa
     7081 gactctctcg atgaagtttt ctctaagctt tagatcgttc agccggccct cttcttcgac
     7141 cggcaagagc ggccactggc tcatcagcgc cgcggcgaac aggccgcggc cttgccgctc
     7201 cggcatgcct ttgctgttat agactttgac acgggccata ccgcagctcg gggagttttt
     7261 cttcaacaca tagccggata ggtgtttttc agtgatatcg cgcactcgtt ttgcggcgaa
     7321 gcgatgcatc gcgtcagtcc agtctttccc cgatttctcc gcaatcattc gtggatggct
     7381 cggcgcgccg actaaccgca ccgtttcacg cggcacgccc atgccgactt cgacttcggg
     7441 acagatcggt accaaatcga cgaatttgcc aaagagctcg gtgaggaaga gatcgcgttt
     7501 gtggcccccg tcgaagcgaa cctgatgccc gagaaggcaa gcgctgattg caacttt
//
LOCUS       DASQKS010000077         4993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_464056, whole genome shotgun sequence.
ACCESSION   DASQKS010000077 DASQKS010000000
VERSION     DASQKS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464056"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VE170_02020"
     CDS             complement(<1..507)
                     /locus_tag="VE170_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYJ14233.1"
                     /translation="MDFPRYRPRRLRRNEKLRAMVRETSLAPANLIYPLFVGPGRDKV
                     QPVASMPGVAQLSVDHAVRECHEAHSLGIPAVILFGLPEHKDALGSEAYGDDGVVQQA
                     IREIKSKLPELLVITDVCLCEYTDHGHCGVIKNGDVDNDSTLELLAKESLSHARAGAD
                     IVAPSDMMD"
     gene            complement(528..1337)
                     /locus_tag="VE170_02025"
     CDS             complement(528..1337)
                     /locus_tag="VE170_02025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYJ14234.1"
                     /translation="MTNVAGTVSKEKPFARRRIVVTRARKQAGGLAQRIEVLGGEVIE
                     FPTIEIQAPASFAGLDRAVQHIETYPWIIFTSVNTVAPFLERLKIAEKSVADLGNAQV
                     AAIGSETTKRLAAAGIAVAVIPNRYQAEGLLESLNGAELAGKRVLIPCAANARDVLPE
                     TLRRWGATVDVVEAYKTVAPAFDAAALRQRLQHRDIDMVTFTSSSTVSNFVQLCGGGS
                     LAAIAGTAALASIGPITGKTIEELGGNVTVMARQSTVDGLIDAMIEYFSVI"
     gene            complement(1342..2253)
                     /gene="hemC"
                     /locus_tag="VE170_02030"
     CDS             complement(1342..2253)
                     /gene="hemC"
                     /locus_tag="VE170_02030"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYJ14235.1"
                     /translation="MKPVRIGSRGSMLALAQTSWVKQQIESCYPDLQVEMSIIKTSGD
                     RVIDRPIVAVSGKGVFTKEIEDALLNQTIDIAVHSMKDLPTELPDGLAIVAVPVREDA
                     RDVLVTKRSLKLADLPRGARLATGSLRRQAQLLRHRGDLAITPIRGNVDTRLRKLDDG
                     EADGLIMAAAGLKRIGRADRIGEYLPDEICVSAVAQGALAIEARADGGLRELLGFLHD
                     SVAEAETVAERALLRRLSGGCHVPIGARARAAGALLSMIAIVASPDGATLCKAKLTGA
                     TSEAEQLGERLAEELLKQGADKILAAS"
     gene            complement(2250..3539)
                     /gene="hemA"
                     /locus_tag="VE170_02035"
     CDS             complement(2250..3539)
                     /gene="hemA"
                     /locus_tag="VE170_02035"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYJ14236.1"
                     /translation="MQKEIVIVGLNHRSASIEVRESVAFESDYLADALKRLHNYSSID
                     EGVILSTCNRVEVVAAALDCASAYHDIGSFLAEQGAHRTGVALAEHTYQYRGTEAVRH
                     LFRVAASLDSMVVGEPQILGQLKQHYDAAQQAGSVGTILHRLFHRSFSVAKRVRNETG
                     IGGGAVSVSSVAVDLAKRIFDRFDDKTVMLIGAGKMGDLMARHLQRHGVRSLMVTNRT
                     FDRAVELAARIQGSPIRFEDFPQYLKMADLVIGCTGALEVLVDAAQVEKVLRERKHKA
                     MFFIDVGDRRNFDGKINDLDNVYLYNIDDLKNVAEENLQGRSSEAARAETIVGEEVQS
                     FVRWIGSLEQVPTITALRRRFDDIRRGEIDKSLGGGLKNLSPEQRTAVEEMTTAMINK
                     LLHAPIAQLKRRNDENDDDDAALYVAALRKLFDLEGK"
     gene            complement(3624..4442)
                     /locus_tag="VE170_02040"
     CDS             complement(3624..4442)
                     /locus_tag="VE170_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HYJ14237.1"
                     /translation="MEVTLVRLTAVIYLLTAASYFYFVFRKDTASKLPALALASGLVF
                     HTAALLVRFANEGFAAVALMGEALLFKSWLMVAVYLLMQLKYRLTVLGGIVAPLAFLM
                     CVAALAFGKAGAEIPPGLESFWLPLHVTLAFLGNAVFALAFGVSLIYLLQERHLKHKK
                     MTALLKRFPPLESLDRLNLILLVWGFPLMTLGILTGSLWAGIHWGDYWSWEPRQISSG
                     LAWLFYGAILHGRITAGLRGKKAALLTMVGFCVVLGYFLWGDTFFPSRHGGRFQ"
     gene            complement(4694..>4993)
                     /locus_tag="VE170_02045"
     CDS             complement(4694..>4993)
                     /locus_tag="VE170_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14238.1"
                     /translation="LEAGTKIIPAGYRVNLPAEKIRPFVAAQARAAASPVKKSTGGVR
                     KVAAQPESAPRASKTPSNRPPKSVPAASSPGAKHRTKDRVIKDSTDRQSLKIAAR"
BASE COUNT         1049 a         1540 c         1390 g         1014 t
ORIGIN      
        1 gtccatcatg tccgacggcg cgacgatatc ggcgccggcg cgggcatggg acaacgactc
       61 tttcgccagt aattccaaag tgctgtcgtt atcgacatcg ccatttttga tcacgccgca
      121 atggccatga tcggtgtatt cgcacagaca aacatcggtg atgacgagca actcgggcag
      181 cttgcttttg atctcgcgaa tcgcctgctg aacgacgcca tcgtcgccgt aggcctcgct
      241 accgagggcg tccttgtgtt caggcagtcc aaatagaatc accgcgggga tgcccagcga
      301 atgagcctca tgacactcgc gcacggcatg gtccactgac aactgcgcca cccccggcat
      361 tgacgcaacc ggctgcactt tgtccctacc gggaccgacg aacaagggat agatcaaatt
      421 ggccggagcc agcgaagttt cacgcaccat tgcccggagt ttttcattac gccgcaaccg
      481 gcgcggccga taccttggaa agtccaatgg ggtgctccga ctcaatgcta gattacgcta
      541 aaatattcga tcatcgcgtc gatcaatccg tcgacagtgg actggcgcgc catcaccgtc
      601 acattcccgc ccaactcttc aatagttttg ccggtgatcg ggccaatgct ggcaagcgcc
      661 gcagtgccgg cgatcgccgc cagcgagccg ccgccgcaga gctgaacgaa attactgact
      721 gtgctggaac tggtaaatgt caccatgtct atgtcgcggt gctgcagccg ttggcgcagc
      781 gccgccgcgt cgaacgccgg ggcaacggtc ttataggctt cgaccacatc aaccgtagcg
      841 ccccaccggc gcaacgtttc gggcagaaca tcgcgcgcat tggcggcgca gggaatcaag
      901 acccgcttac cggctagttc cgcgccgttt aaggattcca gaagtccctc cgcttgatag
      961 cggtttggaa taaccgccac ggcaattccc gcagcggcaa gccgcttggt ggtctcggaa
     1021 ccgatcgccg ccacctgcgc gttgcccaaa tctgccaccg atttctcggc gatcttcaag
     1081 cgttcgagaa acggcgcgac ggtattgacg ctagtgaata taatccatgg gtatgtttcg
     1141 atgtgttgca ccgccctatc caacccagca aagcttgccg gcgcttgaat ctcgatggtc
     1201 gggaactcga ttacctcacc accgagcact tcgatgcgtt gagccagacc accggcttgt
     1261 ttgcgtgcgc gagtgacgac gatacgtcgt ctggcgaacg gcttttcctt cgatactgtc
     1321 ccggccacat tggtcatggg atcaactcgc agccaagatc ttatccgcac cctgcttcag
     1381 cagctcctcc gccagccgtt ctccaagctg ctcggcctcg ctggtagcgc cggtcagctt
     1441 ggctttacac aacgtcgcgc catcgggact ggcgacgatg gcgatcatgg ataaaagcgc
     1501 ccctgcggcg cgggcccgcg cgccgatcgg cacgtggcaa ccgccgctca aacggcgcaa
     1561 gagcgcgcgt tccgcgacgg tctccgcctc ggccaccgaa tcatggagga agcccagcaa
     1621 ctcgcgtagt ccgccgtcgg cgcgcgcttc gatggctaac gcgccttgag ccaccgcgct
     1681 gacacagatt tcatccggca aatattcgcc gatccggtcg gcgcggccga ttcgctttag
     1741 cccggcggcg gccatgatta aaccatcggc ttcgccgtcg tcgagcttac gcaagcgggt
     1801 atcgacgttg ccgcggatcg gtgtaatggc taaatcgccg cgatggcgta acagttgtgc
     1861 ttggcgccgc agactgccgg tggccaagcg ggcgccgcgc ggcagatcgg caagtttaag
     1921 acttctctta gtcaccagca catcccgggc atcttcccgc accggcacgg ccacgatcgc
     1981 taagccatcc ggcagttctg tcggcaaatc cttcatcgag tgcaccgcga tgtcaatagt
     2041 ctggttcagc agcgcgtcct cgatttcctt ggtgaacacg cctttaccgc tcaccgccac
     2101 gatcggccgg tcgattacac gatcgccgct ggtcttgatg atcgacattt cgacctgaag
     2161 atccggatag caagactcga tctgctgctt cacccagctg gtttgggcga gggcaagcat
     2221 gctgccccga ctgccgatgc ggaccggctt catttaccct ccaaatcgaa gagtttcctg
     2281 agggcggcga catacagcgc ggcgtcatcg tcgtcgttct catcattgcg gcgcttgagt
     2341 tgcgcgatcg gcgcatgcag caatttgttg atcatcgccg tggtcatctc ctctaccgcc
     2401 gtgcgctgtt caggggaaag gtttttcagc ccgccgccga gagacttgtc gatttcgccg
     2461 cggcgaatgt cgtcgaatcg tcggcgcaac gcagtaatcg tcggcacctg ttccaatgaa
     2521 ccgatccaac gcacaaaact ttgcacttct tcgccgacta tggtttccgc tctcgccgct
     2581 tcgctggagc gtccctgaag attttcctca gcgacatttt tgagatcgtc gatgttatag
     2641 agatagacgt tgtcgagatc gttaatctta ccgtcgaagt tgcgccggtc gccgacgtcg
     2701 ataaaaaaca tcgccttgtg cttacgctcg cgcaacacct tttcgacctg cgcggcgtcg
     2761 accaatacct ccagagcgcc ggtgcagccg atcaccagat cggccatttt caaatactgg
     2821 ggaaaatctt cgaaacgaat cgggctgccc tgaatcctgg cggccagctc cactgcccga
     2881 tcgaaggtgc ggttggtgac catcaggctg cgcacgccgt ggcgttggag atgacgcgcc
     2941 attagatcac ccatcttccc ggcgccgatc agcatcacgg tcttgtcgtc gaagcgatcg
     3001 aatattcgtt tcgccaggtc cacggccacc gaacttaccg acaccgcgcc gccgccgatg
     3061 ccggtttcgt tacgcacccg cttggctacc gagaacgaac gatgaaataa ccgatgcagt
     3121 atcgtcccca ccgagccggc ttgttgagca gcgtcgtagt gttgttttaa ttgtccgagg
     3181 atctgcggct cgccgaccac catggaatcg aggctggcgg cgacgcgaaa cagatggcgc
     3241 actgcctcag tgccgcggta ttggtaagta tgctccgcca acgccacgcc agtacgatgc
     3301 gccccctgtt cagcgagaaa gctaccgatg tcatggtacg cgctagcaca atccaatgcc
     3361 gcggcaacca cctcgacgcg attgcaagtg gacaggatca cgccttcgtc aatagacgaa
     3421 taattgtgca gacgcttcag agcatcggcc aaatagtcgc tttcgaacgc cacgctctca
     3481 cgcacctcga tggaagcgct gcgatggttc agcccgacga tgacgatttc tttttgcacg
     3541 gcttatctac aatcggccac gctgctcgca gcgggaaacc tggtacggtc taatatggga
     3601 gcgatcatcc tctttgacta gggttattga aaccggccgc catgccggct ggggaaaaac
     3661 gtatcgcccc agagaaaata tccaaggacg acgcagaacc cgaccatggt caagagcgcc
     3721 gctttcttgc cgcgcagacc ggcggtaatc cggccgtgca gaatcgcgcc gtaaaataac
     3781 cacgcgaggc cggaagaaat ctgccgcggt tcccacgacc agtaatctcc ccaatgaatt
     3841 ccggcccaaa gactgccggt gagaataccg agggtcatca aggggaaacc ccagaccagc
     3901 agtatcaggt tgagccgatc gagggactcc agcggtggaa agcgctttaa cagcgcggtc
     3961 attttcttgt gcttgagatg gcgctcttgc agcaggtaga taagactaac gccgaaggcg
     4021 agggcgaaca cggcgttgcc gagaaacgcc agcgtgacat gcaacggcaa ccagaaagac
     4081 tccaggccgg gcggaatttc cgcgcccgct ttgccgaacg ccagcgccgc cacgcacatc
     4141 agaaacgcca gcggcgcgac gatgccgccc aacaccgtga gtcgatactt gagttgcatc
     4201 agtagataaa cggcgaccat caaccaactc ttgaacaaca gcgcttcgcc cattaaagcc
     4261 accgcggcaa agccttcgtt ggcaaaacgc acgagcagcg cggcggtatg aaagaccaaa
     4321 ccactcgcca gcgccagcgc gggcagcttg gatgctgtgt ccttgcgaaa gacgaagtag
     4381 aaatagctgg ccgcagtgag cagatagatc accgcggtga gcctgaccaa ggttacttcc
     4441 atagggcaga aatcattata ggctaacccg tcctgccttc aagccagctg acgacggcaa
     4501 tataggcaac cgtgaagcta ctgaaatcga ctctatttcg gccggtcaaa acagcaattc
     4561 acgcttggct tttgtttttc tcccctagat gccccgtgcg cgggatacgg aaggtttctt
     4621 aagctgcggt tgatagcctt cacagttgcc gagtgaggaa caataacgtt tcaggaatgc
     4681 agttggcagc gcttcagcgt gctgcaattt tcaatgattg tcgatccgtc gagtctttga
     4741 tgacgcgatc cttcgtccgg tgtttggcac ccggagaact cgcggcggga acgctcttgg
     4801 gtgggcgatt agaaggcgtc ttgcttgcgc gtggcgctga ctctggttgc gccgccacct
     4861 tgcgcacgcc accggtcgat tttttcaccg gcgacgccgc ggcgcgagcc tgggccgcca
     4921 cgaacggccg gatcttttcc gccggtagat tcacccggta accggcgggg attattttcg
     4981 tgcccgcttc gag
//
LOCUS       DASQKS010000078         8364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_885606, whole genome shotgun sequence.
ACCESSION   DASQKS010000078 DASQKS010000000
VERSION     DASQKS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885606"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VE170_02050"
     CDS             complement(<1..405)
                     /locus_tag="VE170_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYJ14239.1"
                     /translation="MLALLFVSLVFLGSCTAVSLKAGFSDVGALVEDRGGLRIFWNNG
                     TDLDREACEKLQSLLSNKLKVDDAVQIALLNNRELQTIYSDLGVAQADLVQAGLLKNP
                     LFDTSVRWPMPGGGKPELDLSAVVSFLDIFYLP"
     gene            complement(427..888)
                     /locus_tag="VE170_02055"
     CDS             complement(427..888)
                     /locus_tag="VE170_02055"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HYJ14240.1"
                     /translation="MKRHILKILSSVLAFSACAAYQPQPLSQDHPAHGDAASAPRQAP
                     SLTLAYTRADMPSQQAARAKAGEQNVRAEAPAGAMVQQTVEGEGKVVATVASASQIVV
                     EHGEIKGFMEAMTMGYRVDPPALLEGIKAGDKVRFTIDVPSKVIIKIDRSK"
     gene            complement(905..1591)
                     /locus_tag="VE170_02060"
     CDS             complement(905..1591)
                     /locus_tag="VE170_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HYJ14241.1"
                     /translation="MSSKLSASGLAWALTLFASAAIGDAAERGSPRSQGAVQAAEPHH
                     PRNWRFTMPKGDAAKGRTVFEKFECYYCHRINGEAFPEPTDSAPELSQMGALHPLEYF
                     TESVMHPDGVVPRNLRNRDGSSPMSKDHLERMTLRELIDLSSYLAGLKPPTLARSVNG
                     VGKIVAVVPQSQEVVIDHEEIEGFMDAMTMGYKLASPTQLQGLKTGDVVNFSIDTGKR
                     VITKIDKSKK"
     gene            complement(1696..2091)
                     /locus_tag="VE170_02065"
     CDS             complement(1696..2091)
                     /locus_tag="VE170_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14242.1"
                     /translation="MKRVLAIAACLSILYAGALWAFAGCESLITASAGHDHGDLNSES
                     HHHGDASVPRHSDPGKIHCPNLFDEFLVGSRTSIEPNPRVSVSVGYESFRCSPLVEGL
                     SPCRFDPGPPGPIVSAHISRQVLLSVIRI"
     gene            complement(2109..2912)
                     /locus_tag="VE170_02070"
     CDS             complement(2109..2912)
                     /locus_tag="VE170_02070"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYJ14243.1"
                     /translation="MYPIIFQLGPLTIYSFGAFMALAALTAVWLVRAELKRRGYNPEL
                     ASTLVFAAAVGGLIGARLLFIVEEWQGFLAAPMDYILTGAGFTWYGGFFGGAAAVSWA
                     VRKHKIACFETADMAAPALALAYGIGRLGCHFAGDGDWGAVTDVPWGVAYTRAIVGWR
                     DPSTGIPYPPGVVVHPTPIYEFLQSVFVFAILWSLRKKGFAAGTIAWLYLILAGLMRF
                     IVEFWRINPPVGFGFSEAQWFSLLLMVVGIVMLITRPPRAHLESGAIAR"
     gene            complement(2959..3678)
                     /locus_tag="VE170_02075"
     CDS             complement(2959..3678)
                     /locus_tag="VE170_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYJ14244.1"
                     /translation="MIATGIIVFSAFSALLLEGTSPALAADTVAKGRVVGVVSIAGVL
                     EQPKPLPVFKSRSFCGARVPNETLLVTADGRLRNAAVILRPLSGGVVAPPKKLVLDNQ
                     HCAFTPHVQVATRGSELLLKNSDPILHTVHARLGSETLFNVGLPKWRQVSKRLDRTGV
                     IKIDCDVLHTWMSAAIIVTDSPYSFVSNDNGEFYFDEIPPGNYEIDIWHERLGRHSQR
                     VVVRDNGGTDVNVVFSAESKR"
     gene            3755..4192
                     /gene="dtd"
                     /locus_tag="VE170_02080"
     CDS             3755..4192
                     /gene="dtd"
                     /locus_tag="VE170_02080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYJ14245.1"
                     /translation="MRTVIQRVSEATVTVDGETISRIGAGLCILLGVGKEDTQENAAG
                     LADKVTNLRIFADDMGRMNRSALDIGGAILVISQFTLYGDCRRGNRPSFIDAAPPALA
                     QELYQAFTNRLRAAGLSVVTGQFQAHMKVALINDGPVTLFLQA"
     gene            4195..4647
                     /gene="trmL"
                     /locus_tag="VE170_02085"
     CDS             4195..4647
                     /gene="trmL"
                     /locus_tag="VE170_02085"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HYJ14246.1"
                     /translation="MQVVLFEPEIPPNTGSVARLCAATLTPLHLIEPLGFKIDDKHLK
                     RAGLDYWPFVDLHVHKDWQQFLDQVKPTRLLYFTKKAERAYTSIDYRQDDFLVFGPET
                     RGLPQEMWQDLAACALRIPMSGAGVRSLNLSNAVAIVVYEGLRQLGMT"
     gene            4724..5956
                     /locus_tag="VE170_02090"
     CDS             4724..5956
                     /locus_tag="VE170_02090"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HYJ14247.1"
                     /translation="MSVWAVRNRPAVLLGMLALVWLATVGLLSNGYAAEADTTPPPKE
                     TFNLILPFKNLTVGQGQEVTMDTEIVNRRKDPVEVAIALDGVPKGWDVNFNSRYPSFP
                     IRSVQVAGGEKSTTIEFKAKIPEATRPGVYQVKVNAKDTNGSTQYAETLNITVTSKKV
                     ETGGLKLTSQYPVLSTAAGQTLKFTVDLKNETNKPLTTSLSAQSPPGWTLRFKPQFGD
                     QQISSIQLKENGSETLSVEIDTPAKADAKEQPVSITARSGPFEASATVKVSLKGTQDL
                     KMGSLGGTLNTTVTAGKKTSVDFVVGNAGTAAVRNLSFVTKKPSDKWVVEFKPDKIDA
                     VEPGQVREIKMEVLAPDRTIAGDYLMTLTANSPEVTKSVEFRVTVSTPTVWGWIGFGI
                     VGLVVLGLAIVFFRLGRR"
     gene            5953..6885
                     /locus_tag="VE170_02095"
     CDS             5953..6885
                     /locus_tag="VE170_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14248.1"
                     /translation="MSTVVDTKSLTKRYREKLAVNALNLTIQEGEIFGFLGPNGAGKT
                     TTILMLLGLTEPTSGEVSVCGFNPTSESLEVKKRVGYLPESPGFYDDLSARENLLYMA
                     RLNRIPEEEAQRRTSEVLEQVGLSEDGRRLVKEFSRGMKQRLGIAEVLVKKPTAIILD
                     EPTLGIDPDGAIRILELIKNLNLQHGLTVMLSSHQLQQVQQICSRVGIIVKGKLIVQG
                     ETDTLGKTILKGRNWNFLLEVTGNVDGFDRELQSIVGVDEIEKRSHGWFLRCTQDVRG
                     EIVSAVTRRGLGLLQLRSEDPTLEEIYLKYFREA"
     gene            6889..7812
                     /locus_tag="VE170_02100"
     CDS             6889..7812
                     /locus_tag="VE170_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ14249.1"
                     /translation="MSVVFWKELADHFSSRRFMILMILIVFSGLWAAYATGQAIRANT
                     DTVPTQYVFLLLLTSSGDTLFSLATFLGFFGPLVGITLGFDSISGEYARGTLSRVLSQ
                     PIYRDSLINGKFFAGLTTVAVLWGSILLLVIGLGITLLGFPPNAEELWRMIIFTVVGV
                     FYVGFWLALAMLFSLLFQKTVTAALASIAVWLFLSLFVAVISGAVASMVVPDASTPEA
                     LSHKSDIENIVGRISPSTLFNESVGILLNPAARVFGMALQSQAEGILPTPLALDQSLR
                     LIWPHITTLFALVAVCFGISYIIFMRAEIRA"
     gene            7977..>8364
                     /locus_tag="VE170_02105"
     CDS             7977..>8364
                     /locus_tag="VE170_02105"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ14250.1"
                     /translation="MAYADLREYVAALEKHGKLKRVTKEVDKDWEIAAVCRQLFKKIP
                     PNKRPALVFENVKGFDMPVVAGVLGASREIYALGLQSETVEGINRKWDHALGNPIPPR
                     MISAKDAPCKENIRHGKEVDITKLPVP"
BASE COUNT         1911 a         2364 c         2269 g         1820 t
ORIGIN      
        1 cggcaaataa aaaatatcaa gaaaactcac caccgcgctc aaatctaact ccggcttgcc
       61 accgccgggc atcggccatc ttactgacgt gtcgaagagc ggatttttga gcagtcccgc
      121 ttgcaccaaa tctgcctggg cgacgccgag atccgaatag atagtttgga gctcgcgatt
      181 gttgagcaag gcgatctgca cggcgtcatc gacctttagc ttgttactca gtaacgattg
      241 aagcttctcg caagcttctc tatccaaatc ggtgccgtta ttccaaaata ttcggagtcc
      301 gccccgatct tcgaccagcg cgccgacgtc ggagaatccg gcttttaggc tgactgcagt
      361 gcaactgcct agaaagacca gagatacaaa aagcaatgcc agcatccggg cgcacctccc
      421 ctaactttat ttcgaccggt caatcttgat gatcacttta ctcggcacat cgatggtgaa
      481 tcgaacttta tcgccagcct ttattccttc cagaagggcg ggcgggtcca cccggtaacc
      541 cattgtcata gcttccatga atccctttat ttccccatgc tcgacgacga tctggctggc
      601 gcttgccacg gtcgcgacaa ctttaccctc gccctctacg gtctgctgta ccatcgctcc
      661 ggctggcgct tcagctcgga cgttctgctc acctgcctta gcccgtgcgg cttgttgcga
      721 cggcatgtcc gcccgggtgt atgccagcgt cagcgaaggc gcctggcgcg gcgctgacgc
      781 cgcgtcgcca tgagcgggat gatcttggct caacggttgc ggctgatacg ccgcacaggc
      841 tgaaaacgcc aagacactcg ataaaatttt taatatatgt ctcttcatga ctggcctcag
      901 aaattcattt tttagatttg tcgatcttgg taatcacccg tttgccggta tcgatggaga
      961 agttcacgac gtcgcccgtt ttcaatccct gtagttgcgt gggcgaggcc agtttgtatc
     1021 ccatggtcat cgcgtccatg aagccctcga tttcttcatg gtcgatgaca acttcctggg
     1081 actggggcac cacggccacg atcttgccga caccgttcac cgacctggcc aacgtcggcg
     1141 gcttgagccc cgccaaataa ctcgagagat cgatcaactc gcgcagcgtc atacgttcca
     1201 gatgatcctt ggacatcggc gagctaccgt cgcgattgcg cagattgcgc ggcaccacac
     1261 cgtcaggatg catcaccgac tcggtgaaat attccaacgg gtgcagcgcg cccatttggc
     1321 tcaactccgg cgcgctgtcc gtcggctccg gaaacgcctc gccgttgatc cggtggcagt
     1381 aatagcactc gaacttttca aacacggtgc gccctttggc cgcatcaccc ttgggcatgg
     1441 taaagcgcca attgcgcgga tgatgcggtt cggcggcttg cacggctcct tggctcctcg
     1501 gtgagccacg ctcagctgca tcgccgattg ccgcgctggc aaagagcgtg agcgcccagg
     1561 ccaagcccga cgccgataat ttgctcgaca tagtttcccc caaatcgata ggagaaacgg
     1621 ctgatcggac aagcccgatc ggtcgtgtga ctcaaaaagt gaacagtgaa tgaagaaatt
     1681 accgcgagcg cggtattaga tgcggattac ggaaagcaat acttgacgcg aaatatgggc
     1741 cgaaacaatc ggaccgggcg gtccgggatc gaatcgacaa gggctgagtc cctcgactaa
     1801 cggcgaacag cggaaggatt cgtaaccgac agatacactg acacgcgggt tgggttcgat
     1861 ggaggtacgc gacccgacga gaaattcatc gaaaagattt gggcagtgga ttttccctgg
     1921 atcggaatga cgcggaaccg aagcgtcgcc atgatgatga ctttcgctat tgaggtcccc
     1981 gtggtcatgc cccgccgacg ctgtgatcag actctcgcat ccggcaaacg cccacagcgc
     2041 tccagcatag agaatcgaca agcaggcagc gatagctagt acgcgtttca taaataactg
     2101 caaataagct agcgcgcaat cgcgccgctt tcaaggtgcg ctcgcggtgg gcgagtaatt
     2161 aacatgacga taccgacgac catcagtaaa agactgaacc attgcgcttc ggaaaagcca
     2221 aagccaaccg gcggattgat gcgccagaac tcgacaataa agcgcatcag accggcgaga
     2281 attaaataga gccaagcgat ggttcccgcc gcgaagcctt tttttcgtag cgaccaaaga
     2341 atcgcaaaca cgaaaacact ttggagaaat tcatagatgg gtgtcggatg aaccaccacg
     2401 ccgggcggat aaggaatgcc ggtgctggga tcgcgccagc cgacgatcgc tctagtgtag
     2461 gcgacccccc aaggaacatc ggtcacggca ccccaatcgc cgtcaccggc aaagtgacaa
     2521 ccgagccggc cgattccata ggccagagcc aacgcgggcg cggccatgtc ggcggtctcg
     2581 aaacaggcta tcttatgttt tctaaccgcc caactcaccg ccgcggcgcc gccaaagaat
     2641 ccgccatacc aggtaaatcc cgcgccggtg agaatgtaat ccattggcgc ggcgagaaag
     2701 ccttgccatt cttcgacgat aaacagcaaa cgcgcgccga tgagcccgcc gacagcagcg
     2761 gcgaagacga gtgtcgaggc gagttcgggg ttgtatccgc gacgcttgag ttcggccctg
     2821 accagccaaa cggcggtcaa tgccgccagc gccatgaatg cgccgaaact gtagatggtc
     2881 agcgggccga gctggaaaat aattggatac atgaacgcgc ctcggtaaaa ctagcagatc
     2941 gtttaaacaa aaacagcact agcgtttact ttccgctgaa aaaaccacat tgacatcggt
     3001 gccgccgttg tcacggacga cgacgcgttg ggagtgccgc cccaaccgct catgccagat
     3061 gtcaatttcg taattacccg gcggtatctc gtcgaaataa aactctccgt tatcgttgct
     3121 gacgaaggag taaggactgt ccgtgacgat gatcgccgcg ctcatccagg tatgaagcac
     3181 gtcgcaatcg atctttataa ctccggtgcg gtcgaggcgt ttgcttacct ggcgccattt
     3241 gggcaaaccg acgttgaaca gggtttccga gcccaagcgc gcatgcaccg tgtgcaggat
     3301 cggatcgcta ttcttgagta agagttcgct gccccgggtc gcgacttgga catggggcgt
     3361 aaaggcacaa tgttgattgt cgagaacaag ttttttcggc ggcgccacaa cgccaccact
     3421 gagcggccgg agaatcaccg cggcgttacg caagcgcccg tcggcagtga ccagcaaagt
     3481 ttcattgggt accctcgcgc cgcagaacga ccggctctta aacactggca gcggcttagg
     3541 ttgctccaat actccggcaa tgctgacgac gccgacaacc ctacctttgg ctacggtatc
     3601 cgccgccagg gcaggcgatg tgccttctaa gagtagcgcg gagaacgcgc taaagacaat
     3661 aattccagtg gcgatcattg atctaattat tgggacctgt acgcttcatt gttaacaatt
     3721 gcgatgctac gtcaatactt gttctaaagc caaagtgcgc acggttatac aaagagtcag
     3781 tgaagcgacg gtcaccgtag acggcgaaac catcagccgg atcggcgccg gcttgtgcat
     3841 tcttcttggc gtcggcaaag aggataccca ggaaaacgcc gcgggcctag ccgataaggt
     3901 gacaaatttg cggattttcg cagacgacat gggcagaatg aatcgctccg cgttggatat
     3961 agggggcgca attctggtga tatcgcagtt cactttgtac ggcgactgtc gcagaggcaa
     4021 tcggccctcg tttatcgacg cagcgccgcc ggcactggcg caggaactct atcaagcatt
     4081 caccaaccgt ctgcgcgctg ccggcctgag cgtcgtcacc ggccagtttc aagcgcatat
     4141 gaaagtggcg ctgatcaatg acgggccggt gactttattt ttgcaagctt aaccatgcaa
     4201 gtcgtgcttt tcgaaccaga aattccgccc aacaccggca gcgtcgcgcg cctatgtgcc
     4261 gctaccctga cgccgctgca cttgatcgaa ccgctgggat ttaagatcga tgacaagcat
     4321 ctcaaacgcg ccggcctcga ttattggccg tttgtcgacc ttcatgtgca taaggactgg
     4381 cagcagtttc tcgaccaggt gaaaccaaca agactgcttt attttaccaa gaaagcggag
     4441 cgggcttata cctctatcga ttatcggcaa gatgactttc tcgtcttcgg tccggagact
     4501 cgtggcttgc cgcaggaaat gtggcaggac ctggctgcat gcgcgctacg tattcccatg
     4561 agcggcgcag gagtgcgtag tttgaattta tccaatgcgg tggccatcgt cgtttatgaa
     4621 ggtttgcgtc aattgggcat gacttaatta ccacgaggcc ttgacggtga aacttataac
     4681 ggttaattat cggcaacaaa tttgttctga gggggttgat tatatgagcg tttgggctgt
     4741 aagaaatcgc ccggcggtat tgctcggtat gctcgcgctg gtttggctcg ccacggtggg
     4801 tttgctttcc aacggttacg ccgctgaagc ggacacgact cccccgccga aagaaacttt
     4861 caatttgatc ttgcccttta aaaatctcac ggtcggccaa ggccaagaag tgacgatgga
     4921 taccgagatc gtcaaccggc gcaaggaccc tgtcgaagtc gctatcgcct tggatggggt
     4981 gccgaaaggc tgggatgtta attttaattc ccgctacccg agctttccga tccgctcggt
     5041 gcaagtcgca ggcggcgaaa aatcgacgac cattgaattc aaggcgaaga tccctgaagc
     5101 aactcgtccc ggagtctacc aagttaaggt taatgccaaa gacaccaatg gttcgaccca
     5161 gtatgccgaa accctcaata tcaccgtcac gtcaaaaaaa gttgagaccg gcggtctcaa
     5221 gctgaccagc cagtatccag tgttgagcac cgccgcaggc cagactctca agttcaccgt
     5281 cgatctcaaa aacgaaacca ataaaccgct gaccacctcg ctgtccgctc aatcccctcc
     5341 cggttggacg ctccgtttca agccgcaatt cggcgatcaa cagatctcgt cgatccagct
     5401 caaagaaaac ggctcggaga ctcttagcgt cgagatcgat acaccggcaa aggccgacgc
     5461 caaagaacag ccggtgtcaa ttaccgcgcg ctccggcccg tttgaggcat cggctactgt
     5521 caaagtcagc ctcaagggca cccaagatct taagatgggc tccctcggcg gcaccttgaa
     5581 cacgacggtt accgccggca agaaaacgtc ggtggatttc gtcgttggca acgccggcac
     5641 cgcggcggtc cgcaatttga gcttcgtcac caaaaagccc agcgacaagt gggtcgtcga
     5701 gttcaagccc gacaaaatcg atgccgtcga gcccggccag gtgcgagaga tcaagatgga
     5761 agttctcgca ccggatcgca ctatcgccgg cgactacctg atgaccttga ccgccaattc
     5821 gcccgaagtc accaagtccg tggaattccg tgtgactgtg tcgacgccga cagtttgggg
     5881 ctggatcggc ttcggcattg tcggtttagt ggtgcttgga cttgcgatcg tatttttccg
     5941 cttaggtcgt cgatgagcac ggttgtcgac accaaaagtc tgactaaacg ctaccgtgaa
     6001 aaactcgcgg tgaacgcgct taatttgacg attcaagagg gcgagatttt tggttttctc
     6061 ggtcccaatg gcgcgggcaa gaccacgacc atcttaatgc tcctgggctt gactgaaccg
     6121 acttccggcg aagtttcggt gtgcggcttc aatccgacca gcgagtctct cgaggtcaaa
     6181 aaacgggtcg gctacctgcc ggagagtccc ggcttctacg acgacctaag cgcgcgcgag
     6241 aacttgctct acatggctcg gctcaaccgc atcccggaag aggaagcaca gcggcgcacc
     6301 tccgaggtgc tcgagcaagt cggtctcagc gaggatggcc ggcgcctggt caaagaattt
     6361 tcccgcggta tgaagcagcg ccttggcata gccgaggtat tggtcaagaa acccacggcg
     6421 atcattctcg acgaaccgac cctcggcatc gaccccgacg gcgccatccg cattttagaa
     6481 ttgatcaaga acttgaatct acagcatggt ctgacggtca tgctgtcgtc gcatcaattg
     6541 cagcaggtgc agcagatttg cagccgggtc ggtatcatcg tcaaaggcaa gctgatcgtt
     6601 cagggcgaaa ccgatacctt gggcaaaacg atcttgaaag ggcgcaattg gaattttctt
     6661 ctcgaagtaa ccggcaacgt cgatggtttc gatcgagaac tgcagagcat cgtcggcgtc
     6721 gatgaaatcg agaaacgtag ccatggctgg tttctccgct gtacacagga cgtgcgcggt
     6781 gaaatcgtga gcgcggtgac tcgccgcggc ctgggcctgc tacagttgcg ttccgaagac
     6841 ccgacccttg aagagattta tttgaagtac tttcgcgagg cgtaaaaaat gagcgtggtg
     6901 ttctggaaag agcttgccga tcactttagc agccgccgtt tcatgatcct gatgatcctg
     6961 atcgtcttct ccgggctttg ggcggcttac gccaccggac aggcgattcg cgccaacacc
     7021 gataccgtgc cgacgcagta tgtcttctta cttctgctca cctccagcgg cgacacgtta
     7081 ttttccctgg caaccttcct aggcttcttc ggtcccttgg tcggcatcac tctgggcttc
     7141 gattcgatta gcggcgagta tgcacgcggc acccttagcc gcgtgctgtc gcagcctatc
     7201 taccgcgaca gtctgatcaa cgggaagttt ttcgccggcc tgaccaccgt cgctgtatta
     7261 tgggggtcga ttctgcttct ggtgatcggt ctgggtatta ccctcttagg gtttccgcct
     7321 aatgccgaag agctctggcg catgattatc ttcaccgtgg tcggagtgtt ttacgtcggc
     7381 ttttggctgg cgctggcgat gctcttttcg ctgctgtttc agaaaaccgt taccgcggct
     7441 ctcgcctcga tcgcggtttg gttatttctt tcgctgttcg tggctgtgat cagcggcgcc
     7501 gtcgccagca tggtcgttcc cgatgccagc acgccggaag cactgtcgca caaatcagat
     7561 attgaaaaca tcgtcgggcg tatttcgccc agtactctgt tcaatgaaag cgtcggcatt
     7621 ctgctcaatc cagcagcccg ggtgttcggc atggctctgc aaagccaagc tgaggggata
     7681 ctgccgacgc cgttagcact cgatcaaagc ctaaggttga tttggcccca tatcaccacg
     7741 ctgttcgcgc tggtggctgt ctgtttcggc atttcgtaca taatcttcat gcgcgcggag
     7801 attcgcgcct agacaaacct cctatatccc cgcgattttt tttcggcgta ggagcgcaag
     7861 cccaagcgag tgcgcttctt cttcgccaaa tcatttcata ttggaatcgt tgatagttaa
     7921 acgattttct ggctagtata caaaaacttt taccctttgg ctgagacgga ggatttatgg
     7981 cctatgccga tctacgcgaa tatgtcgcgg cgctggagaa acatggcaag ttgaagcgcg
     8041 tcactaagga agtcgataaa gactgggaaa tcgccgcggt gtgccgccag ctattcaaga
     8101 aaatcccacc gaataagcgc cccgcgttgg tcttcgaaaa cgtcaaaggc ttcgacatgc
     8161 ctgtcgtcgc cggcgtgctc ggcgcgtcgc gcgagatcta tgctctgggc ctgcaatccg
     8221 aaacggtcga aggcatcaac cgtaaatggg accatgccct gggcaatccc attccaccgc
     8281 gcatgatttc agccaaggat gcgccctgca aagaaaacat ccggcacggc aaagaagtcg
     8341 acatcaccaa actgccggtg ccga
//
LOCUS       DASQKS010000079         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4663099, whole genome shotgun sequence.
ACCESSION   DASQKS010000079 DASQKS010000000
VERSION     DASQKS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4663099"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            501..1313
                     /locus_tag="VE170_02110"
     CDS             501..1313
                     /locus_tag="VE170_02110"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HYJ14251.1"
                     /translation="MATMIADECINCGACEPECPNNAITQGDPIFVIDPKLCTECVGF
                     HDYEACAAVCPVDVCITDPNNVESEEALIQRARELHPETNFGDSFESRFRKGEGKAAA
                     AADTAQTPGTPARPSPVSAPVATGAVSAFAEADVNYVELPAIDSWNIPLRCYKCNGVH
                     EQNVKNFTIGNVIFCPHCHKSTVVRDNLNYHIRTLLKDSYDQWENEQREFVERREREL
                     AEFVARRAKDASAFEGHQKRERERIRAQLDTIGDTYDAPGKPYKKGTRFSWD"
     gene            1326..1799
                     /locus_tag="VE170_02115"
     CDS             1326..1799
                     /locus_tag="VE170_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14252.1"
                     /translation="MFYSWLHLIALVVYLGAVVGFWLMLLPAVGVLEKHDERVRYFVR
                     GLKFYNPLQVGSLGIILFTGAFQLTELKAAYRETFIEYLAYNLGLKLLFAFLMVMFSV
                     YQALGIGHRFVRRIEGGDDVTPEQLRSIISRMTGANWFILLFAAITLWLGLRLQR"
     gene            1874..2809
                     /locus_tag="VE170_02120"
     CDS             1874..2809
                     /locus_tag="VE170_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14253.1"
                     /translation="MANKKLRVIYRSNSHAPLWLVADKSGSWQKNGLEVDTSPQLIRE
                     KAVESLKNGTVDLISGNHHNLYVRNAKNGEDFVHLAQPTNNWTENKLIVRPGIRSVQD
                     LKGKKIVADRLTSHAGLNIWLFLKQEGLDVDRGDIELCELRGSSEERWQRVLSGEYDA
                     TFVTIPHDSRAAKAGAHVITVRAIPMIRGVTLTTTSSFVKSHPEEIRLLTRGLIDAIH
                     FFITRKPETLEILKEHATPILKLQTDQEVETLYEEWAHSLEKKPYPSLEGIANVFQLA
                     VRRNPEIAGYNPLALWNTHFVRELDDSGYIDKLYS"
     gene            complement(3057..3848)
                     /locus_tag="VE170_02125"
     CDS             complement(3057..3848)
                     /locus_tag="VE170_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ14254.1"
                     /translation="MKSTASKYFIGAGVSAVLLMGQGCLATRDWVRENVNDPLSGRIT
                     QHDGRLDGHDGQIGTLGTRMTGVEGKLGQFEGRLGQIEGRVGQVDAKADKALSSIANL
                     RLERKIVIDMKDGANFAFNSANLPPQAQREIDGFLSDLKGDVAGADGTIFLVAGHTDS
                     TGNEEYNYELGKKRADAVSRYLITKKSMDPMKVIPVSYGQSAPVAENNNNQGRSKNRR
                     VEILVYREGITSQAAAAPASQQQQQPAAPRSSQTTQPDGDRITQR"
BASE COUNT          945 a         1213 c         1061 g          960 t
ORIGIN      
        1 tggtatttga acaaacctgc gtcgacaccg atgtacgcca ccgccgtgcc gacttcccaa
       61 cccgcgtagg ccaaacgcag tttcttcgga ctttccgcag cgagggaaac agtagcgatg
      121 cagacaagca atgcgaacgc gaatcccatc ggaaatatcc gaggaaagtt gaacagccgg
      181 aagataatca tgcgggcatc atagcccacc aaaaccgacg cgccaagagg gtggcgctcg
      241 cgccgccgtg gctgcgactt tttattggcg ccatccatga gtggcgtcgg agcacatgtg
      301 aaatacccca agccgggacg tagccgcagc taaagtagaa tctcatttca ctggctgccg
      361 aatcgaccca cagcagcaca tcggcgagtc agaaatgcct ctgatattcc agtcttaggg
      421 aacttgaaga tttgcaacgc caattctttt catttacgat gaggctaatt ccacgctagt
      481 cattttggag gtagaatttc atggcaacga tgatcgccga cgaatgtatc aactgcggtg
      541 cttgcgagcc cgagtgcccc aacaatgcga taacgcaagg cgatccgatt ttcgtcatcg
      601 atccaaagct atgcactgaa tgcgtcggct ttcacgacta cgaggcctgt gccgcggtct
      661 gtccggtgga cgtctgcatc accgatccga acaatgttga atccgaagaa gctttgatcc
      721 aaagagcccg cgagcttcat ccggaaacca actttggcga cagtttcgaa tcgcgcttcc
      781 gtaagggtga aggtaaggcg gcagcggcag ccgatacagc gcaaaccccc ggcacgcccg
      841 cccgcccaag cccggtttcc gcgccggtcg cgactggagc ggtctcggca ttcgccgaag
      901 ccgatgtgaa ctatgtcgaa ctgccggcca tcgattcctg gaatattccg ctgcgctgct
      961 acaagtgcaa tggcgtccat gaacaaaacg tcaagaactt taccatcggc aatgtgatct
     1021 tttgccccca ctgccacaag tctacggtag tccgggacaa tttgaactat catatccgca
     1081 ccctgctcaa agactcctac gaccaatggg aaaatgagca acgcgaattt gtcgaacggc
     1141 gcgagcgtga gctggcggaa ttcgtcgcac ggcgcgctaa ggacgctagc gcgttcgagg
     1201 gccaccagaa acgcgagcgc gagcgcatcc gcgcacagct ggacaccatc ggcgacacct
     1261 atgatgcccc cggcaaacct tacaagaagg gcacgcgttt tagctgggac tgatcgcggc
     1321 agccgatgtt ctactcctgg ctccatctga tcgccctggt ggtctacctg ggcgcggtcg
     1381 tcggtttttg gctgatgctg ctacccgcag tcggcgtgct ggaaaagcat gacgagcgcg
     1441 tgcgttattt cgtccgcggg ctcaagtttt acaatccgct ccaggtcggt tccctgggaa
     1501 tcattttatt taccggagcg tttcagctca ccgaactgaa agcggcctac cgagaaacgt
     1561 tcatcgaata tctcgcctac aatcttggcc tgaagttgct gtttgcattt ctgatggtca
     1621 tgttcagcgt ttaccaggcc ctcggcatcg gccatcgttt cgtccggcgg atcgaaggcg
     1681 gcgacgatgt gacacccgag caactgcgct cgatcatttc ccgcatgacc ggtgccaact
     1741 ggtttattct gcttttcgcc gccattaccc tctggctcgg cctccgcctg cagcgctagt
     1801 ccggcgctgg caaaaccact ctcactgatg ctatgacccg attagcaagg tttacatttg
     1861 agaggaaatt ttcatggcca acaagaaact ccgggtcatt taccgttcta attcacatgc
     1921 gccgctttgg ctggtggccg acaaatctgg gtcctggcaa aagaatggcc ttgaagtcga
     1981 cacttcgccg cagctgatcc gggaaaaggc cgtcgagtcg ctcaaaaacg gcaccgtcga
     2041 tctgatctcg ggcaatcatc acaacctcta tgtgcgcaac gccaaaaatg gcgaagactt
     2101 cgttcacttg gcccagccga cgaataactg gacggaaaac aagcttatcg tcaggcccgg
     2161 tattcgctcg gtgcaggacc tcaaaggcaa aaaaatcgtc gccgacaggc ttacctccca
     2221 cgccggcctc aacatctggc tgtttctgaa gcaagagggt ctggatgtcg accgcggcga
     2281 tattgaactc tgcgagttac gtggttcgtc cgaagagcgc tggcaacggg tcctaagcgg
     2341 ggaatacgac gccaccttcg ttaccatccc ccatgacagc cgcgccgcca aagccggcgc
     2401 ccatgtcatt accgtgcggg cgattccgat gattcgcggc gtcaccctga ccactacgtc
     2461 gagctttgtg aaaagtcatc ccgaagagat tcgtttattg acccgggggc tgatcgacgc
     2521 catccacttt tttattaccc gcaagccgga gaccttggag attctcaagg aacatgccac
     2581 gcccatcctc aagctccaaa ccgaccagga ggtcgaaacg ctctatgaag agtgggcgca
     2641 ctctttggag aaaaaaccct atccgtccct cgaaggaatc gccaacgtct ttcagctcgc
     2701 cgtgcgccga aatcccgaga ttgccggtta taaccctctg gcgctgtgga acactcactt
     2761 cgtgcgcgag ctggacgaca gcggctacat cgataagttg tatagttaaa tcgtatgccg
     2821 cagttttaaa gcaagtcgtg gtcaaatcgg cgtctgccat tacatagcgc cgagcgcttt
     2881 cgccttgtac gatcgatcgc gactaattaa aaggttactg gcaaccgccg ccggttgacg
     2941 ccttcttcgt agggccctct cacctgcgca atgctgcatt taagcacgca ccaataaaaa
     3001 aaggcggagt cgatgactcc gccttttttg tctgcccgcg ttttcttata aagaaattag
     3061 cgctgggtga ttctgtcacc gtccggctgg gtcgtctgcg acgagcgcgg tgcggctggc
     3121 tgctgctgtt gctgcgacgc cggagctgcc gccgcttgcg aggtgatacc ttcgcggtaa
     3181 actaggattt caacccggcg atttttagat ctgccttgat tgttgttatt ttccgcgacc
     3241 ggagcgctct ggccgtacga aaccgggatt accttcatcg gatccatgct ctttttggta
     3301 atcaggtaac ggcttactgc atcggcgcgt tttttgccga gctcataatt gtactcttca
     3361 ttacccgtgc tgtcggtgtg gccggcaacc aggaagatcg tcccgtccgc accggcaaca
     3421 tcgcctttga gatcgctcaa aaagccatcg atttcacgct gtgcttgcgg cggcaaattg
     3481 gccgagttga acgcgaagtt cgcgccgtct ttcatatcga tgacgatctt gcgttccaag
     3541 cgtagattgg cgatcgagct tagcgctttg tcggctttcg catcgacctg acctacccga
     3601 ccttcgatct ggccaagacg gccttcgaat tgcccgagct tgccttcgac accggtcatg
     3661 cgagtgccga gagtgccgat ctggccatca tggccatcca aacgtccatc gtgctgggtg
     3721 attcgcccag acagcggatc attgacgttt tcacgaaccc aatcgcgagt cgccaaacac
     3781 ccttgaccca tcagcaatac cgcactcaca ccggcgccta taaaatattt actagcggta
     3841 cttttcatgc aatcctcctc tttgttgttg gttaagaata tttattcccc ggtaatcact
     3901 ttgcaaattc agaatctatc cctatggtaa ctactatcaa ttttcgtacc acgaacctaa
     3961 aaatcgtaag gaactaatat tacactactt tctttagaat taagtcgccc cgtaggcaag
     4021 ctcgcaggag tttccataca gcgctgaagg tttattgcac gctatcacct ctcgacgttt
     4081 gaaatcctcg ccttttcgca aacttggcat gtagtcatta atcgtaggta cgcccaagtc
     4141 gcgctcaccc actcaagcgt cgatgggtag tctctcata
//
LOCUS       DASQKS010000080         8328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2143216, whole genome shotgun sequence.
ACCESSION   DASQKS010000080 DASQKS010000000
VERSION     DASQKS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143216"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1547
                     /locus_tag="VE170_02130"
     CDS             90..1547
                     /locus_tag="VE170_02130"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ14255.1"
                     /translation="MGELAYTDFRGFIEEAKKISDFRIIEGADWDAEIGALVESTAEL
                     VAQPPMLIFDKIKGYPAGFRVCSLPYAAYRRVALALGLPTDKTKLETLRLAARKVRSA
                     EPIAPKEVGTSPLFENKLTGADVDMLKFPALRFHENDGGRYFGPGGGLINQDPDNGYV
                     NTGTYRMQLHDKNTLGIWMSPGQNGRQICAKYWEKGKSCPVAASYGQHPMMFMPSYTK
                     FPYGRSEMEIAGGLMGRPLEVLRGPITGLPIPASSEIAIEGEIPPPSEEARAEGPFGE
                     WPGYYSGGTGGTGENQPIIRIKAIYHRNDPILEDEAPLWPGAVKMDLHISAGVLWDQL
                     ESAGIQDIAGVYNHTSYMTVVSIRQKYAGHAKQAGQAALACAAANRNGRYVVVVDEDI
                     DPTNMKEVLWAMMTRVDPPTNIDIIDGCWSTPLDPRMPPEKRDNKDHTNGRAIFYAVR
                     PYHWKDKFPKVSRSSRELREKTIAKFKGVIPFPGS"
     gene            1620..2900
                     /locus_tag="VE170_02135"
     CDS             1620..2900
                     /locus_tag="VE170_02135"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ14256.1"
                     /translation="MISREENQLLTQTNAGTPCGELLRRYWQPVALSEELKTGGAPVA
                     VTLMGEELVIFRDDQGRVGVLDAHCCHRGADLTYGRLEDGGLRCIYHGWLYDVHGKIL
                     EQPGEPNGGEHRDAYRQPSFPCREIGGVILAYLGPGEPPLLPNYEFLQVPDAQRFVSK
                     IHHSCNYLQSNEGNIDPVHLSFLHRNIELTETDKKRRVPGSASSPNSLFGADIAPRID
                     VELTDFGLRIFSIRNIEDDKIYFRTSNFIMPNFSTFPGQTAAEGYSVNWHVPIDDHSH
                     WKFVIVFSRKRALDRSMMVRGRDELQPDYRMIRNKANRYMQDRESMHDKSFSGLGYNF
                     QAQDACVIEGAGTIQDRTKEHLVTSDKAIVAARKLLLKGIQDVKEGRDPLHVVRDPRK
                     NDFAHLAVISEVAPKTADIRSIVQQKIAEQSLSA"
     gene            2992..4179
                     /locus_tag="VE170_02140"
     CDS             2992..4179
                     /locus_tag="VE170_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ14257.1"
                     /translation="MSEILRLGMAGLGVASTQILPPIAKLPYIKITAAADMRADAVAK
                     FREQYNAEGYTRVEELCASANVDAVYIATPNSLHAEHAIIAAQHKKHIIVEKPMAMNL
                     AECDAMNEAADKYGVKLLCGHTHSFDPPIRKIREIVKSGELGKLCMINSWNYNEFMYR
                     PRMKHELAMTRGVVLNQGPHHVDVVRLIGGGMVRSVRAMTGIWDPAREWEGSYTCYLE
                     FADGTPATLVYSGYGFFDTAELFDWVGEGGQHRPPATNLTVREKLREVRTAEEEEELK
                     EGMRFGGKREGEFSHVSDGERKQPFFGHTVVSCEKGDIRQTPDGLRIYGETEQREIKL
                     PPGSRGREAEVEELYDAVVKGRPVFHGGRWGAATLEVCLAMLESAQQRKEIMLSHQVP
                     SPE"
     gene            4241..5371
                     /locus_tag="VE170_02145"
     CDS             4241..5371
                     /locus_tag="VE170_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14258.1"
                     /translation="MRHRLAWYGIILCLWSSGFSQASESKPAWQAEWERTVKAAEEEG
                     QLTVYIAGYGALIDSGVFQKAYPKIKVTSVTGSGTQLAPRIVAERRGEKYLADVYSGG
                     GGSIYQNLYLGKMLDPLKPALILPEVIDPTKWWEGKQRYADREGRYIIVYEANVAAGA
                     SPAYNSNLINPKDYKSLWDFLNPKLKGKIVSPDLRRVRGSGPSFQYLYYHADLGPEFL
                     RRFYVEMDVTLTADGRQAMDWLAGGKFALSMPTQGTYTVKAKAQGLPVDEFDPHHFKE
                     GASISAAFGQLALMNRAPHPNAAKVFVNWLLSREGQSAFQRIISTPGEAKNSRRIDVP
                     KDHIPAPERRLDGGKYFDPDEVGTRDVTPVTKLMDEVLAGRK"
     gene            5444..6613
                     /locus_tag="VE170_02150"
     CDS             5444..6613
                     /locus_tag="VE170_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ14259.1"
                     /translation="MLTAEENEMMTKVGPGTPAGELLRRYWHPVAIAADLTAENPTKF
                     LRILGEDLVLFQTTGGEVGLLHDRCAHRGVSLSYGRVEERGLACAYHGWLYDIKGNCL
                     AMPAETADSSFCQTVKQKAYPVQKLAGLYWAYFGPLPAPVIPRYDVWARTDGWHWLRA
                     LPQLDCNWLQAMENSVDTAHLHVLHQELVTDGFKVESTTRGTIDNLAKFEVEEFVHGV
                     IKRRVFKDGKVEEHPLLFPTVLRQANRTQIRVPIDDTHTWIVYVHFVPDSAEPHANKD
                     EVPVSYRKPFKNPLGARHPDTKFRLDEVDAQDFMAWETQGPVLDRTRERLAASDRGVV
                     VYREMLAREIKKVAQGLEPMNVFRDPNHPIIDTKMDHSLEVGKAGSVTYHVPPKT"
     gene            6835..8022
                     /locus_tag="VE170_02155"
     CDS             6835..8022
                     /locus_tag="VE170_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14260.1"
                     /translation="MAQGMLAERRADKSKQKKQEAKIMLRRLLPFVGVALLIACGSSI
                     AAQAKPAWQAEWEKTLEAAKKEGQLSIYISGYEENLPDFQKEYPEIKVAPITGRGSQI
                     GQRLLAERRADKFLADLVSSGGVTTYQQLFPAKVFDPIKPALLLPEVNDLTKWYQGRH
                     HYADPENQYIISYVGTATYGSVSYNTKLVDVKDFKSYWDLLNPKWKGKIISRDIRVAG
                     PGSGNARLFYYLPDVGPSFIRKLYSEMDVTLFRDYRQSADWLAVGKAAICFFCEVDVS
                     KQQGLPVDTFGPGVFKEGAGLVQQFGTLGLVNRAPHPNAAKVFINWLLSRRGQIALQK
                     NMLKTENPTDSLRIDIPKDEIPILQRRFEGVKYLDTSRPEWQEMKPILDVMNEALKAA
                     GKS"
     gene            8131..>8328
                     /locus_tag="VE170_02160"
     CDS             8131..>8328
                     /locus_tag="VE170_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14261.1"
                     /translation="MAQPKLVWQQEWEKTLEAAKKEGQVVVYIGGYEAVLPDFEKEFP
                     EIKLVAVPARGAQIVQRLTSER"
BASE COUNT         1850 a         2242 c         2578 g         1658 t
ORIGIN      
        1 tgtaggggct tattacgata cgctcggtgg tgggaagcgg cggtctgcgt aacgaaatga
       61 atgacgttaa tttgttagga gactctaaaa tgggcgagct tgcttatacg gactttcgcg
      121 gcttcattga agaagcgaaa aagatcagcg actttcggat catcgagggc gccgactggg
      181 acgctgaaat cggcgcattg gtggagtcca ccgccgagct ggtagcgcag ccgccgatgt
      241 tgatcttcga caagatcaaa ggttatccgg cgggattccg ggtgtgcagt ttgccctacg
      301 cggcctaccg gcgcgtggcc ttggcgctgg gattgcccac cgacaagact aaattggaaa
      361 ccttgcgtct ggcggcgcgc aaggtgcgca gcgccgagcc gattgcgccg aaggaagtcg
      421 gcacctcgcc gctgttcgag aacaaactca ccggcgccga tgttgatatg ttgaaattcc
      481 cggcgctgcg ttttcacgag aacgacggcg ggcgctactt cgggcccggc ggcggtttga
      541 ttaaccagga tcctgataac ggctatgtca acaccggcac ctaccggatg cagctgcacg
      601 acaaaaacac cctcggcatc tggatgagtc ccggacagaa cggccggcag atttgcgcca
      661 agtattggga gaagggcaag agctgtccgg tggcggcgag ttacggccag catcccatga
      721 tgttcatgcc gtcgtatacg aagttcccgt acggccgctc ggagatggaa attgccggcg
      781 gcttgatggg ccggccgctg gaagtcttgc gcgggccgat taccggtttg ccgattccgg
      841 cgtcctcgga gatcgccatc gaaggcgaga tcccaccgcc ttcggaagag gcacgcgcgg
      901 aggggccgtt tggcgaatgg ccgggatatt attccggcgg caccggcggc accggcgaga
      961 accagccgat tattcgcatc aaggcgattt atcaccgcaa cgacccgatc ctcgaagacg
     1021 aggcgccgct ctggccgggc gcggtgaaga tggatctgca tatttccgcc ggcgtgctgt
     1081 gggatcaact ggaaagcgcc ggcattcaag atatcgccgg ggtgtacaat catacttcct
     1141 acatgaccgt ggtatcgatt cggcagaaat acgccggcca tgccaagcag gcagggcaag
     1201 cggcgctcgc ctgcgcggcg gccaatcgta acgggcgtta cgtggtcgtg gtcgatgagg
     1261 atattgatcc gaccaacatg aaggaagtgt tgtgggcgat gatgacgcgg gtcgatccgc
     1321 cgaccaatat cgatattatc gacggctgct ggagcacgcc gcttgatccg cgcatgccgc
     1381 cggaaaagcg cgataacaag gatcacacca acggccgggc catcttctat gccgtgcggc
     1441 cgtatcattg gaaagataag tttcccaaag tcagccggtc gagccgggag ctgcgtgaga
     1501 agacgatcgc caagttcaaa ggcgtgatcc catttccggg gtcgtgaggg tggccggccg
     1561 gtccccaata gccctcgccc cggtcgccga ggcggtcagt gattcgagga attttagata
     1621 tgatcagtcg ggaggaaaat caacttttaa ctcaaaccaa cgccggcacg ccgtgcggcg
     1681 aactcctgcg ccgctactgg cagccggtgg cattgtcgga agagctgaaa accggcggcg
     1741 cgccggtggc ggtaacattg atgggcgagg agttggtgat tttccgcgac gaccagggac
     1801 gggtcggtgt gctcgatgcg cactgttgtc atcgtggcgc cgacttgacc tacggtcggt
     1861 tggaagacgg cgggctgcgt tgcatttatc acggctggct ttacgacgtt cacggtaaga
     1921 ttctcgaaca gccgggcgag cccaacggcg gcgagcatcg cgatgcctac cgccaaccgt
     1981 cttttccttg ccgcgagatt ggcggcgtga ttctagccta cctggggccg ggcgagccgc
     2041 cgcttttgcc gaattacgag tttcttcaag ttcccgacgc tcagcgcttc gtctcgaaga
     2101 ttcatcattc gtgcaattac ctgcaatcga atgaaggcaa tattgatccg gtgcatctgt
     2161 cgttcttgca ccgcaatatc gaactcaccg agaccgacaa gaagcggcgc gtgcctggca
     2221 gcgcaagctc gcccaattcg ctgttcggcg ccgatatcgc gccgcgcatc gacgtcgagc
     2281 tgaccgactt cggcttgcgg attttttcca ttcgcaacat cgaagacgac aagatttatt
     2341 ttcgcacgtc gaatttcatc atgcccaact tcagcacctt ccccggtcag accgcggcgg
     2401 agggctattc cgtcaattgg catgtgccga tcgacgacca cagtcactgg aagttcgtca
     2461 tcgtcttcag ccgcaagcgc gcgctcgacc gctccatgat ggtgcgcggg cgcgacgagt
     2521 tgcagcccga ttatcgtatg atccggaaca aggcgaaccg ttacatgcaa gaccgggagt
     2581 cgatgcatga caaatctttc agcggtctcg gctacaactt ccaggcgcaa gatgcctgcg
     2641 ttattgaagg cgccgggacg atccaagacc gcaccaagga acatctggtc acatcggaca
     2701 aggcgatcgt cgcggcgcgc aagctgttgc tcaagggtat tcaggatgtc aaggaaggcc
     2761 gcgatccgct gcatgtcgtg cgcgatccgc gtaagaatga ctttgcgcac ctcgccgtca
     2821 tttcggaggt cgctccaaag accgcggata ttcggtccat cgtgcaacaa aaaattgccg
     2881 agcaaagttt gtccgcgtag tgaatttaac cattcgtaca aaatgcgctc tcacctctgt
     2941 ccctcccaga gggagaggag ggaagaaagc ccttcagcga aggaacaata aatgagtgag
     3001 attcttagat tgggcatggc tggtttgggc gtggcgagca cgcagatctt gccgcccatc
     3061 gccaagttgc cgtacatcaa aatcaccgcc gcggcggaca tgcgcgcgga tgcggtggcg
     3121 aagtttcgcg agcagtacaa cgccgaaggt tacaccaggg tcgaagagtt atgcgccagc
     3181 gccaacgtcg acgctgtcta catcgctacg cccaactcgc ttcatgccga acatgcgatc
     3241 atcgccgccc agcacaaaaa gcatatcatc gtcgaaaaac ccatggcgat gaatctggcg
     3301 gaatgcgacg ccatgaacga ggcggcggat aaatacggcg ttaaattgct gtgcggccac
     3361 acccatagct tcgatccgcc gatccgcaag attcgcgaga tcgtcaaaag cggcgagctc
     3421 ggcaagctgt gcatgatcaa cagctggaac tacaacgagt tcatgtaccg gccgcgcatg
     3481 aagcatgagc tcgccatgac ccgcggcgtg gtgctcaatc agggaccgca ccatgtcgac
     3541 gtggtgcgct tgatcggcgg cggcatggtg cgtagcgtgc gcgccatgac cggtatctgg
     3601 gacccggcgc gggagtggga agggagctat acctgttatt tggaatttgc cgacggcacg
     3661 ccggcgacac tggtttatag cggttacggt ttcttcgaca ccgccgaact gttcgattgg
     3721 gtcggcgagg gcggccagca tcggccgccg gcgactaatt tgacagtgcg agaaaaatta
     3781 cgcgaggtgc gcacggcgga agaagaagag gagttaaaag aaggcatgcg ctttggcggc
     3841 aagcgtgagg gcgagtttag ccatgtctcg gacggtgagc gcaagcagcc gtttttcgga
     3901 cacacggtgg tgagctgcga gaagggcgat attcgccaga cgccggatgg tttgaggatc
     3961 tacggtgaaa ccgagcagcg ggaaatcaag ctgccgccgg gcagccgcgg ccgcgaggcg
     4021 gaagtcgaag aattatatga cgcggtggtc aaagggcggc cagtatttca cggtggccgc
     4081 tggggcgcgg caaccttgga agtttgctta gccatgttgg agtcggcgca acagcgtaaa
     4141 gaaatcatgc tatcgcatca ggtgccgagt ccggagtagg atggggacag ttcaagctcc
     4201 aagcgtacaa tgttcaaggt cggagtcgga gagattcgac atgcggcatc gcttagcgtg
     4261 gtatggaatc attctgtgtt tgtggtcgag cggcttctct caagcctccg agtccaagcc
     4321 cgcatggcaa gcagaatggg agcggacggt caaggctgcg gaggaagaag ggcagttgac
     4381 ggtctacatc gccggctatg gcgcgttgat cgattcgggg gtgtttcaga aggcttatcc
     4441 caaaatcaag gttaccagcg tcaccggctc aggcacccag cttgcgccgc gcatcgtcgc
     4501 cgagcggcgc ggcgaaaaat atctcgccga tgtttacagt ggcggcggcg gctcgatcta
     4561 tcagaatctt tatctcggca agatgctcga tccgctcaag ccggcgctga tcttgcccga
     4621 agtgatcgat ccgaccaagt ggtgggaggg caagcaaaga tatgccgacc gcgaagggcg
     4681 ctacattatt gtctatgaag ccaacgtggc ggcgggggcg agtccggctt acaacagcaa
     4741 tctgatcaat cccaaggact acaagtcctt atgggacttt ctcaacccca aactcaaagg
     4801 caagatcgtc tctcccgatc ttcggcgggt gcgcggttcc gggccgtcgt tccaatatct
     4861 ttattaccac gccgatctcg gcccggagtt tctgcgccgt ttttacgtcg agatggacgt
     4921 taccttgacc gccgacgggc gtcaggcgat ggattggctg gcgggaggta agttcgcctt
     4981 gtccatgccg acgcaaggta cttacacggt caaagccaag gcgcaggggc tgccggtgga
     5041 cgaattcgat ccgcatcatt tcaaagaggg cgcgagcatc tcggcagcct tcggccaact
     5101 tgccttgatg aaccgcgcgc cccatcccaa tgcggccaag gtttttgtca attggctgct
     5161 ctcgcgcgaa gggcaaagcg ccttccagcg gattatcagc acgccgggag aggcaaagaa
     5221 ctcacggcgc atcgatgtgc ccaaggatca cattccggcg ccggagcggc gccttgacgg
     5281 cggtaagtat ttcgatcccg acgaggtcgg cacgcgggat gttacgccgg tgaccaagtt
     5341 gatggatgag gttttagccg gtaggaagta atcgtttggg tcgatgatgt gatcgaggat
     5401 cgaggatagc gcgattatag ggcgatgttt aaggagaaga ggaatgttga ctgctgaaga
     5461 aaatgaaatg atgaccaaag tcggtcccgg cacgccggcg ggggaattgc tgcggcgcta
     5521 ctggcatccg gtggcgatcg cggcggactt gacggcggag aatccgacca agtttctgcg
     5581 catcctcggc gaggatttgg tcttgttcca aaccaccggc ggcgaggtcg ggcttttgca
     5641 tgatcgctgt gcccatcgcg gtgtgtcgct atcctacggg cgggtcgagg agcgcggcct
     5701 tgcatgcgca taccatggct ggctttacga tataaagggc aactgtctgg cgatgccggc
     5761 ggagacggcg gacagcagct tctgccaaac ggtcaaacaa aaagcctatc cggtgcagaa
     5821 acttgccgga ctctactggg cctatttcgg cccgctgccg gcgccggtga tccccagata
     5881 cgatgtctgg gcgcgcaccg acggttggca ttggttgcgc gccttgccgc agctcgactg
     5941 caactggctg caagcgatgg agaattccgt ggacacggct catctgcatg tgctgcatca
     6001 agagctggta accgacgggt tcaaagtcga gagtacgacc cggggcacca tcgataattt
     6061 agcaaagttc gaggtcgagg agtttgttca cggtgtgatc aaacggcgcg tgtttaaaga
     6121 tggcaaagtc gaggagcatc ctctgttatt cccgacggtg ctgcgccagg ccaaccgcac
     6181 gcagattcgc gtgcccatcg acgacacgca cacttggatc gtttatgttc acttcgtccc
     6241 ggactccgcc gagccgcacg cgaacaaaga cgaagtaccg gtcagttacc gcaagccgtt
     6301 caagaacccg ctcggcgccc gccatcccga caccaagttt cgcctcgacg aagtcgacgc
     6361 tcaagatttc atggcttggg aaacccaggg gccggtgctc gaccgcacgc gcgagcggct
     6421 cgccgcatcg gatcgcggcg tggtcgtcta tcgggaaatg ttggcgcgcg aaatcaagaa
     6481 agtagcgcaa ggcttggagc cgatgaacgt cttccgcgat cccaatcatc cgatcatcga
     6541 caccaaaatg gatcattccc tggaagtggg taaggcgggt tcggtgactt atcacgtgcc
     6601 gcccaaaacg tagaggtgtt ccaatcgttc caatcgttca agcggttcaa gtcgtcgacg
     6661 gaagaagaat cggagcgtgg ccgcaaagca tgtcctgagc ttggtcgaga aaatgcaacg
     6721 gggggaaacc agaatccacg agcacttgcg ggcgtagggg cgcgatgcat cgccgcttgt
     6781 gcgccagaga acagaaggac aagccacgga caagtcaggg caaagttaac ggagatggcg
     6841 cagggcatgc tcgccgagcg gcgcgccgat aaatctaagc aaaaaaaaca ggaagccaaa
     6901 attatgttga gaagattgtt gcctttcgtc ggtgtcgcgc tgttaatcgc gtgcggcagt
     6961 tccatcgccg ctcaagccaa gcctgcctgg caagcggagt gggagaagac gctcgaagcg
     7021 gccaagaagg aaggccagct gtcgatttac atcagcggct acgaagagaa tctgccggac
     7081 ttccaaaagg agtatccgga aataaaggtt gcgccgatca ccggccgcgg cagtcagatc
     7141 ggccagcgtt tgctcgccga gcggcgcgcc gataagtttc ttgccgacct ggtgagctcc
     7201 ggcggcgtta ctacctacca acagttgttt ccggcgaaag ttttcgaccc gatcaagccg
     7261 gcgctactgt tgcccgaggt caacgacctg accaagtggt accaaggcag gcaccactat
     7321 gccgatccgg agaaccaata cattatcagt tacgtcggta ccgcgaccta cggctcggtg
     7381 agctacaaca ctaagttggt cgacgtgaaa gactttaaat cctattggga tctgcttaat
     7441 cccaaatgga aaggaaaaat catttcacgg gatattcgag tggccggccc gggttccggc
     7501 aacgcgcggt tattctatta cctgccggat gtcggcccat cttttatccg caagctttat
     7561 tccgagatgg acgtgacgtt gtttcgcgac tatcggcaga gtgccgactg gctggcggtc
     7621 ggcaaagcgg cgatctgctt tttctgcgag gtcgatgtgt ccaagcagca ggggctgccg
     7681 gtggacacct tcggcccggg ggtttttaag gaaggcgccg gcctggtgca gcaattcggt
     7741 actctcggat tagtaaaccg cgcgccccat cccaacgctg cgaaagtgtt tatcaactgg
     7801 ttgttgtcgc gccgaggtca aatcgccctg cagaaaaata tgctcaaaac ggagaatccc
     7861 acagattccc tgcgtatcga cattcccaaa gatgaaatac cgattttgca acgccgcttc
     7921 gaaggtgtga agtacctgga caccagccgg cctgagtggc aggagatgaa gccgatcctg
     7981 gacgtcatga acgaagcgct caaggcggcg ggaaagagtt aagttaatag gtaattgaaa
     8041 ctggagaatt gacgattgag acgacgagcg gccgcgttaa gtagttcttt ttcggcgata
     8101 ggtttctttg gcttggcgat ggttgctttg gtggctcagc caaagcttgt ttggcagcag
     8161 gaatgggaaa aaactctcga agccgccaaa aaagagggcc aggtcgttgt ctacatcggc
     8221 ggttacgagg cggtgctgcc ggattttgaa aaagagtttc cggaaatcaa gctcgtggcc
     8281 gttccggcgc gcggcgcgca aattgtccag cggttgacta gcgagcgc
//
LOCUS       DASQKS010000081         6716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_887258, whole genome shotgun sequence.
ACCESSION   DASQKS010000081 DASQKS010000000
VERSION     DASQKS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887258"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VE170_02165"
     CDS             complement(<1..1070)
                     /locus_tag="VE170_02165"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HYJ14262.1"
                     /translation="MSKATNDALEYLAGAAQRFEVLNKLRKQLKSENEITLARRMIAK
                     LRKEEALLDELPLSQKADLCRQQAELTSKDPELSVNSRHDIALKILGDMFALDSVALN
                     GDAETLGIAGGICKRRWIDLGQYEDLQRAAEYYRRGALGPVGEDGYAQINAAFMQDLL
                     AHASGTESSIRQEANKIREKILNELPALDKVKADNQWWNAATRAEALFGLGRYSEAAA
                     AIKACPKRPETWCLQSAAQQFAVLAQLRDKRPLQNPELRAVFDALLPGATQAVSSSLI
                     GKVGLALSGGGFRASFYHLGVLARLAELDVLRHVDVLSCVSGGSIVGACYWMMLRNRL
                     LKAAPMTQGDYMSLVQDLIEHFT"
     gene            complement(1354..2376)
                     /locus_tag="VE170_02170"
     CDS             complement(1354..2376)
                     /locus_tag="VE170_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ14263.1"
                     /translation="MNALSPARIMDVGLGFWPSKVLLSAIELGLFTELGAKSMTGSEL
                     QEALGLHARAIPDFFDTLVALRFLERDGDGPQARYRNTGETALFLDRNSPQFMAGFLE
                     MANARLYRFWGDLTDGLRTGSAQNEVKHTGAPMFAELYSKPERLELFMDAMSGISAAN
                     FQALAEKFDFSCYRTLCDVGGATGQLSMLVARKHPHLRCISADLPAATAIAERKIAAA
                     GLADQVTAKAIDFFADPLPKADVITMGMILHDWNLEKKLHLIRAAYDALPPGGAFIAV
                     ENIIDDARRENAFGLMMSLNMLIEFGDAFDFTGADFSRWCREVGFSRTEVIPLAGPAS
                     AGVAYK"
     gene            complement(2422..3132)
                     /locus_tag="VE170_02175"
     CDS             complement(2422..3132)
                     /locus_tag="VE170_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ14264.1"
                     /translation="MGTNVHEVAEGIYRINTPVTMAGGPGGFSFNQFLIADEAPLLFH
                     TGPRKMFPLVREAVASVLPVERLRYIGFSHVEADECGSLNEWLEVAAESAPLCGKVAA
                     LVSVDDIADRPARALADGETLALGKHTVRWFDTPHLPHGWECGFLMEEQARTLLCGDL
                     FTQGGAEHPAVTEADILGPSEAFRQMMDYHSHTPHTRAMLERLAAAAPTTLACMHGST
                     WRGDGGKLLIALADALAA"
     gene            3312..3815
                     /locus_tag="VE170_02180"
     CDS             3312..3815
                     /locus_tag="VE170_02180"
                     /inference="COORDINATES: protein motif:HMM:NF017830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HYJ14265.1"
                     /translation="MPIDLTRVIEEVKAEGKTRRVLWQDSESIAFLSSGRKERWDFHI
                     DPADEVTLQVTGVQHLVYRDEAGNEHTADIKAGQILLCPAGVPHSPRLEADSWFIVFE
                     RKRKADEIDYFKWFCNQCGEKVYETSAAVGDYRSDPVGQVYQTFSSDEKLRTCKSCGT
                     IIPAPPT"
     gene            4079..4858
                     /locus_tag="VE170_02185"
     CDS             4079..4858
                     /locus_tag="VE170_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14266.1"
                     /translation="MTDYVKQLTRLAKAYADAERDVIAAFFKKPRKRTGHLAWLKAQG
                     FKEYSAIKPILDALTALYPKIDRGVDRHDYAELTDKLADETKHARLVLDLLEEISGKK
                     IGPQDLTWLPEDRKLAKVRAKYSKSYATLLHGRGKVSSKEIKRRDEDLERAAITLTEG
                     GGGALYLVCSKLKKTGIEAKIAQAFQEILLDEVEHKDSGARSLDGQIKNAADYRRAAE
                     IICAVCGQRLRMRNEQFGYPLKDSQVMALNRSAQAALYGAK"
     gene            4855..5721
                     /locus_tag="VE170_02190"
     CDS             4855..5721
                     /locus_tag="VE170_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYJ14267.1"
                     /translation="MTAVVLALLLQFAFVANATALDLPPLKTTISDFAGMFPPASLEE
                     LEHRLHRFATETANHVAILTVKTLDDTDIDALARQAFNRLPLSDTERNKTVLLVIARA
                     EHEVGFHAGESLQHLLPPPATRDKLREQVGLYINGLRPDLGIHGAVHYLFRALRGDVR
                     VGSQSADNELEEISLSGRGAGAIFALFIAPFLAFFVGGLWGVYATQFGVQRWPRLAMG
                     AIFGGGTAKVVITITAFVGGASDSLWYFIMALAIPLGVFGSCTEFWMSGDWRGIPREK
                     GPRRKPEDNMGI"
     gene            6367..>6716
                     /locus_tag="VE170_02195"
     CDS             6367..>6716
                     /locus_tag="VE170_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ14268.1"
                     /translation="MLRKPIPRSGKLLPVVGLGTWQTFDVGAASPERASLKEVLRKLV
                     QRGGSVIDSSPMYGRSETVVGDLATDLGIHKQLFVATKVWTSGREAGVRQMEESFRRL
                     RVRKIDLMQVHNLL"
BASE COUNT         1483 a         2012 c         1886 g         1335 t
ORIGIN      
        1 gtgaaatgtt cgatcaaatc ctgcactaga gacatataat caccctgggt catgggcgcg
       61 gccttgagca gccgattgcg cagcatcatc cagtagcaag caccgacgat gctgccgccg
      121 gaaacgcagg acagcacatc gacgtggcgc agcacatcca attcggcgag ccgcgccaag
      181 acgcccaaat gataaaacga cgccctaaat ccgccgccgg aaagcgctaa gccaacttta
      241 ccgattaaag aggaactcac cgcctgagtc gccccgggca acaacgcatc gaatacggcg
      301 cgcagctcgg ggttctgcag cgggcgcttg tcgcgcaact gagcaagcac ggcgaactgc
      361 tgcgcggcgc tctgcaagca ccaagtctcg ggccgcttgg gacacgcttt gatcgcagcc
      421 gccgcttcgc tataacgtcc caggccgaac aaggcttcag cccgcgtcgc cgcgttccac
      481 cattgattgt ctgctttgac cttatctaac gctggcagct cattgagaat cttctcgcgg
      541 atcttattcg cttcctgccg gattgaactt tccgttccag atgcatgggc gagcaaatcc
      601 tgcatgaaag ccgcattgat ctgtgcataa ccgtcctcgc cgaccggccc tagcgctcca
      661 cgacggtaat actccgccgc ccgttgcaag tcctcatatt ggcccaagtc aatccaacga
      721 cgtttgcaga tgccgccggc gatccccaag gtttccgcat cgccgttcaa tgccacgctg
      781 tcgagagcaa acatgtcgcc aagaatttta agcgcgatat catgacgcga gttgacgctc
      841 agttccggat ctttactggt cagttcagcc tgctgccggc acaggtcggc cttttggctc
      901 agcggtagct catccagcaa agcttcctct ttgcgcagct tggcaatcat acggcgcgcg
      961 agggtaattt cattttctga tttgagttgt tttctgagtt tgttcagaac ttcgaagcgc
     1021 tgcgcggcgc cggccaaata ctccaacgca tcattagtcg ccttcgacat ggagcctcct
     1081 atcgcgccca aatggaactc acagtaagac cgccggcttg gctcgtcaga aaaaaataat
     1141 tcaataattt ttgtgactta tacattgcca ttatggagaa aatcaaagtt aaaatggctg
     1201 ctcaatcaag tcggccgcta acattcattc attttgcctc gggcggtttc gaagctccta
     1261 agccgctcgc ggagaaactg ctttcgatcg gcgcatggag ggaagcgatg aggtatcaac
     1321 gctaacgcga gagccgcggt cgcagtcatg cggctacttg taagctacgc cggcactggc
     1381 cggcccagcc aatggaatca cctcggtcct gctgaaacct acttcgcggc accagcgaga
     1441 gaaatcggcg ccggtgaagt cgaacgcgtc gccaaattcg atcagcatgt tgagcgacat
     1501 catcagtccg aaggcgttct cccgcctggc gtcgtcgata atgttctcaa cggcgatgaa
     1561 cgcgccgccg ggaggcaagg catcgtaagc cgcccggatg agatgcagct tcttctcgag
     1621 attccagtcg tgcaggatca tgcccatcgt aatgacatcg gcctttggca acggatcggc
     1681 gaaaaagtcg atagccttgg cggtgacctg atcggcaagg ccggcggcgg caatcttgcg
     1741 ctcggcgatc gcggtggcgg cggggagatc cgcggagatg cagcgcaggt gcggatgctt
     1801 cctggcgacg agcatcgaca gttgaccggt ggcgccgccc acgtcacaga gcgtgcgata
     1861 acaggaaaaa tcaaacttct cggccagcgc ctgaaaattg gccgcggaga tgccgctcat
     1921 cgcgtccatg aacaattcga gccgctcggg tttgctgtaa agctcggcga acatcggcgc
     1981 gccagtgtgt ttaacctcgt tctgggcgct gccggtgcgc agcccatcgg tgagatcgcc
     2041 ccagaatcga tatagccgtg cgttggccat ttctaagaaa ccggccatga actggggact
     2101 gttccggtcg aggaacaatg ccgtttcacc ggtattgcga tagcgcgctt gcgggccatc
     2161 gccgtcgcgt tcgaggaaac gcagcgccac cagtgtgtcg aagaaatcgg gaatcgctct
     2221 cgcatggagc ccgagcgcct cttgcagttc gctgccggtc atcgatttcg cgccaagttc
     2281 ggtgaaaagg ccgagctcga tcgccgatag cagaacctta gacggccaaa agcccaagcc
     2341 cacgtccatg attcgagctg gagataaagc attcatcgcc gatattcctt tcctggcaaa
     2401 ttcacgttgt agcgcggcgc attatgccgc cagcgcatcg gcaagcgcaa ttaaaagctt
     2461 gccgccgtcg ccccgccatg tgctgccgtg catacacgcg agcgtggttg gcgcagccgc
     2521 ggcaagtctt tcaagcatcg cccgtgtatg cggtgtatgc gagtggtagt ccatcatctg
     2581 acggaacgcc tcgctcggcc cgagaatatc cgcctcggtg acggctgggt gttcggcgcc
     2641 gccctgggtg aacagatcgc cgcacagtaa agtccgcgct tgttcttcca tcaggaaacc
     2701 gcattcccaa ccgtggggca ggtgcggcgt gtcgaaccag cgcacggtat gttttcccag
     2761 tgccagcgtt tcgccatccg ccagtgcgcg cgccggccgg tcggcgatat cgtcgaccga
     2821 caccagcgcc gcgactttgc cgcacagcgg cgcggactca gcggccacct caagccattc
     2881 gttgagcgag ccgcactcat ccgcctcgac gtgcgaaaag ccaatatagc gtagccgttc
     2941 cacgggcaac acactcgcca ccgcctcgcg caccagcgga aacattttgc gcgggccggt
     3001 gtggaagagc aacggcgctt catcggcgat gagaaactga ttgaacgaaa acccgccggg
     3061 cccgcccgcc atagtcaccg gcgtattgat gcggtaaatc ccctccgcga cttcatggac
     3121 attcgtcccc actgtttatt agtgataagc attttcattc tcctcggata ttaactatct
     3181 cagcacccgg ctttttcaat atcgccgcat ctaatcgact ggccttgcat cttaaatgcc
     3241 gacgaggcgt gaggcaacgc ctgttgcccc gccgctttcg aaagcgctat agtcgtccca
     3301 tggaaaagct catgcctatc gatctcacaa gggtcatcga agaggtgaag gccgaaggta
     3361 aaacccgccg cgtgctgtgg caggattccg agtcgattgc attcttgagc agcgggcgga
     3421 aagagcgctg ggattttcac atcgatccgg cggacgaggt gaccctgcaa gtgaccggcg
     3481 tgcagcatct agtttatcgc gacgaggccg gcaacgaaca caccgctgac atcaaagccg
     3541 ggcagatcct gctctgcccc gccggcgtgc cgcattcacc ccgacttgaa gccgactcct
     3601 ggttcattgt cttcgaacgc aagcgcaaag ctgacgaaat cgattacttc aaatggttct
     3661 gcaaccaatg cggagaaaag gtttacgaga cctccgccgc agtcggcgac taccgttccg
     3721 atccggtggg tcaggtttat caaacgttct ccagcgacga gaaactgcgc acatgcaaaa
     3781 gctgcggcac gataatcccg gcgccgccga cgtgagacaa gcccgttcca gtcgtttcaa
     3841 acgttcaagc cgtttaagtc gtttgaagaa agagattcgg actcagcgaa ttggcacgcc
     3901 gaacttatag ttcttgatgg gggaattaac cgagatacac ttgaagtccc aagattattt
     3961 tccaaacctt ggtggactcc gtggtggaac cattgattgc atattgacgc caaagtcaga
     4021 cagcaggaac tgacttgctt gccaagaaaa ctcgcgcctc aacgctcact cctatctaat
     4081 gactgactat gttaaacagc tcacgcgact cgccaaagcg tacgccgacg cggaacggga
     4141 cgtgatcgcg gcgtttttca agaagccacg caagcgaacc ggccatttgg cttggctcaa
     4201 agcgcaagga tttaaagaat actcggcgat caaaccgatc ctcgatgcgc tcaccgcgct
     4261 ctatccgaaa attgaccgcg gcgtcgaccg gcatgactat gccgagctga ccgataaact
     4321 cgcggacgag accaagcacg cgcgcttagt gttggatttg ctggaagaga tcagcggcaa
     4381 aaaaatcggg ccgcaagatc tcacctggct gcccgaggat cgtaagctcg ccaaggtgcg
     4441 cgccaagtat tccaaaagct acgcgacact gttgcacggc agaggcaagg tcagctcaaa
     4501 agaaatcaaa cgccgtgacg aagatctcga gcgcgccgcc atcacgctca ccgagggcgg
     4561 cggcggcgcg ctctatttgg tttgcagcaa acttaaaaag accggcatcg aagccaagat
     4621 cgcccaggca ttccaagaga tcctgctcga tgaggtcgaa cataaagata gcggagcccg
     4681 ctctctcgac gggcaaatca aaaacgccgc cgactaccga cgcgcagccg agattatttg
     4741 tgcggtgtgt ggccagcggc tgcgcatgcg caacgagcaa ttcggctacc cgttaaaaga
     4801 tagtcaggta atggcattaa accggagcgc ccaggccgcg ctctacggag cgaaatgacc
     4861 gcggttgttc tggcgttgct cctgcagttt gcttttgtcg caaacgcgac agccctcgac
     4921 ctgccgccgc tcaaaaccac gatcagcgat ttcgccggta tgtttccgcc ggcgagtctc
     4981 gaagaactcg aacatcgcct gcaccggttc gccaccgaga ccgcgaacca tgtcgcgatt
     5041 ttgacggtca aaaccctcga tgacaccgac atcgacgcgc tggcgcggca agccttcaac
     5101 cgactgccat tgagcgacac cgagcgcaat aagaccgtgc tgttggtcat tgcgcgagcc
     5161 gagcacgagg tcggttttca tgccggcgag tcgttgcagc atctgctgcc accaccggcg
     5221 acccgcgata aactgcgcga gcaggtgggg ctctacatca acggtctccg accagacctc
     5281 ggcatccacg gcgcggtgca ttatctattc cgcgcgctgc gcggcgacgt gcgtgtcggc
     5341 agtcagagcg ccgataacga gctcgaagaa atatccctga gcggccgcgg cgctggagcg
     5401 atctttgcgc tgtttatcgc accattcttg gctttcttcg tcggcgggct ctggggcgtc
     5461 tatgcgactc agttcggcgt gcagcgctgg cctcggctgg ccatgggcgc gatttttggc
     5521 ggtggcacgg ccaaagtcgt cataaccatt actgcgttcg tcggcggcgc cagcgactcg
     5581 ctctggtatt tcatcatggc gctagcgatt cccctcggcg tgttcggcag ctgcacggag
     5641 ttctggatgt ccggcgactg gcgcggcatc ccgcgggaaa aaggaccgcg gcgaaaacct
     5701 gaagataaca tgggcatatg accgtcagcg agataggatc gcccccggct ttttcgccgc
     5761 attcattgac ggcgatcctg ttcggcttgt tctcgaactt cgtgactcac attgtttcat
     5821 tctagcaaac cgctcctaga ggaacggccc aggtctgatc cgaacactgg acacaacgac
     5881 cgagtcccgg gctcatcgcc agcgcggtaa tctcgtggcc gaacttcaag tacgcaagtc
     5941 gccacgaaac ggcccccaaa gctcgccgcc gccagccgtt gcgcctcagg ccgcgccaac
     6001 accggcagtc gagccagcag acgccgcggc aattccgccg acggcgacac gtgaaggcga
     6061 tgtcgaacgc ttattaggct aagccataca aagcgatgat agccatgctc gcgcggcaat
     6121 gcgcccgtgg agacacagca gaaaagccac gttcgggact accagttgaa ttaacacttc
     6181 tatttctgaa gaataaacgt atggcaatct tgcggcgcaa cttgcgatcc gagaacaatc
     6241 ggtgcggcgg cgatggcccg ggataattca aatgggcaaa ttttgctgtc gcgccgccag
     6301 gtattgcgcg ccggagctgg cgcaagtgcg ttggcactga tgcgaggtcg cgctatggcg
     6361 caatcgatgt tacgtaaacc gatcccacgc agcggtaagt tgttgccggt cgtcggcctc
     6421 ggcacctggc aaaccttcga cgtcggggca gcttcgccgg agcgtgcgtc tttgaaagaa
     6481 gtcctccgca agcttgttca gcgcggtggc agcgtcatcg actcttcacc gatgtatggt
     6541 aggtcggaaa ccgtcgtcgg cgatctcgcc acagaccttg gcattcacaa gcaactcttc
     6601 gtcgcgacca aagtttggac cagcgggcgc gaggcgggtg ttcgccagat ggaggaatcg
     6661 tttcgccggt tgcgcgtgcg caaaatcgac ctcatgcaag tgcacaatct gctcga
//
LOCUS       DASQKS010000082         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2144308, whole genome shotgun sequence.
ACCESSION   DASQKS010000082 DASQKS010000000
VERSION     DASQKS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144308"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VE170_02200"
     CDS             <1..505
                     /locus_tag="VE170_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYJ14269.1"
                     /translation="DLKGKMGIALGGASGNRAIGAMIKAKGEEFVRKLKEQQIKLYSL
                     DAPALVNVIASGEVVASPAIFQSHTWLAASKGAPVEWLPMDFVPNNVGSAAIALKPPN
                     PHGAVLMADFLLSPDGQKVLEKFHYGSATKEQPFKRWRPEHGVATEKYEKDVEHWEKL
                     LKQIGNK"
     gene            complement(884..1738)
                     /locus_tag="VE170_02205"
     CDS             complement(884..1738)
                     /locus_tag="VE170_02205"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYJ14270.1"
                     /translation="MPLNTQALTNKFGVAVTGVDLSKELDEKLFNEIVDIFVKKQVLV
                     FRDQHIEPHHQVAFSRRFGPFEMLFDEDQRVPGFPEVAILSNEKVDGKFIGVVAAGDF
                     WHSDQSYRRETSLATLLYAHKLPKHGGDTEFADMHGAYESLPDDIKKRIDGRMGIHQR
                     SKLGNPRVAVTREGGEEYYKRQAVKNQLHPIVRTHPVSGRKGLYVSPRFTVGIQDMDD
                     AEAQPLLDTLFAYQTAAENVYRHKWTLGDFVMWDNRSVNHQACGGYAMDDIRLLHRTS
                     VKGDQPFA"
     gene            complement(1971..2365)
                     /locus_tag="VE170_02210"
                     /pseudo
     CDS             complement(1971..2365)
                     /locus_tag="VE170_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
     gene            complement(2487..2975)
                     /locus_tag="VE170_02215"
     CDS             complement(2487..2975)
                     /locus_tag="VE170_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HYJ14271.1"
                     /translation="MKRPAVMALLNFFVVLCLFVPPAFSAEATQSKEAKGIAAVVDKA
                     ATLTESKGKTAFSEFKKKGSEWLRGDNYVFIADMNGVILMHPANPELETKSILDLKDA
                     NGKAFMREFVETAKTKGSGWVEYMWPKPGEKTPSKKLSYIKRAKLPEGETVIVGAGIY
                     LK"
BASE COUNT          748 a          937 c          890 g          769 t
ORIGIN      
        1 ggatctcaaa ggcaagatgg gcattgcgtt gggaggagcg tcgggcaaca gggccatcgg
       61 cgcgatgatc aaggccaaag gcgaagagtt cgtcaggaaa ctgaaggagc agcaaatcaa
      121 gctttacagt ctcgatgcgc cagcgttagt caacgtgatt gcctccggcg aagtcgttgc
      181 ctcgccggcg atctttcaga gccatacatg gctcgcggcg tcgaaaggtg cgccggtcga
      241 atggttgcca atggattttg tccccaacaa cgtcggcagc gcggccatcg cgctcaagcc
      301 ccccaatccc catggtgcag ttctcatggc tgattttctc ctaagtccgg acggccaaaa
      361 ggtcctggaa aagtttcact atggcagcgc aacgaaagag cagccgttta aacgctggcg
      421 gccggagcac ggggtcgcca cggagaagta cgaaaaggac gtcgagcact gggagaagct
      481 gctaaagcag atcggcaaca aatagtcatt gggaattctt gtgacactgc cgaggtgaga
      541 ccatctccgg gcctctccta tcgtgagctg gtttgtttga tcgcgcggtc tgcgggtgag
      601 gaagagagcg acgggaagac gaagcgcgat gggcggatcg cgcaagccgt ataccatcac
      661 ggatagaaaa cgcggcgaag ctcctcacga tttaccgaac cttgagactt gtgggcgtgg
      721 cttacgcttc gccgccggga acgcgggtgg acaggtcatg attctccatg acgctttggg
      781 ccttcgtaaa tttcagcatt gtacacatta aaatgtctga ccccggcgcc cgctcattca
      841 tgcctggcat cccttgccga ttattctccc ggcggtcacc gagtcaggcg aatggctggt
      901 ccccttttac cgacgtgcga tgaagcaagc gaatgtcgtc catcgcataa ccgccgcagg
      961 cctggtgatt gacgctacga ttgtcccaca tcacgaaatc gcccagggtc catttatggc
     1021 gatacacgtt ttccgccgcc gtctgataag caaacaacgt gtcgagcagc ggctgggcct
     1081 cagcgtcgtc catgtcctgt ataccgacgg taaatcgcgg cgagacgtaa agacccttgc
     1141 ggcccgatac cggatgagtg cggacaatcg gatggagctg attcttaacg gcctgcctct
     1201 tgtaatattc ttcgccaccc tctcgagtca ccgcgacccg tggattaccc agcttgctgc
     1261 gctggtggat acccatccgc ccgtcgatgc gctttttgat atcgtcaggc agactttcat
     1321 aggcgccatg catgtcggcg aattctgtat cgccgccatg cttaggcagt ttatgggcgt
     1381 agagcagagt cgcgagcgac gtctcgcgtc tgtatgactg atccgaatgc cagaaatcgc
     1441 cggccgcgac cacgccaatg aatttaccgt cgaccttctc attggagagg atggcaactt
     1501 ccggaaagcc cggtacccgt tgatcctcgt caaacaacat ttcgaaaggg ccaaaccttc
     1561 ggctgaaggc aacttgatga tgaggctcga tatgttgatc acgaaaaacc aaaacctgct
     1621 tcttcacgaa gatatcgaca atctcgttga agagcttttc atcgagctct tttgacaagt
     1681 ctacgcccgt aacggcgaca ccgaacttgt tcgtcaaagc ctgggtgttc aacggcatcg
     1741 atcagccctc cccgtttatc tcgatagcaa ataggataat gccgtcaagt cgcggttgaa
     1801 cttattagtt gaggttccga cccaaggccc ccacgacctt cgccgctcca cgccccacgc
     1861 gcattgctat aaatccaggc tgccccgcga gcaagaattt gtcaccgagc agaagttgcg
     1921 cgagtccacc tgcgccaact tactcaggtt gggtgttttg cgttgcctgc ctaatcgagc
     1981 aggtccttag gcacattgcc gccgttggcg gcgatttgcg cgagcacggt cttgtgcagc
     2041 cagatattca tcttcgccga atccccatca gatccgccgg acagccgagc tccgtagcca
     2101 gctccttgcg cgccgcaaag ctgctatcga tctcgagcag cttcagcagg tcgacaatgg
     2161 aagtgcgcca attcagcttc tgcgggttag ctgccgcgcg ctgctccagc tgtgcgacga
     2221 cgtcgacgac cgcgaccggc ttgggcggcg gcggtgcggt cggagccgca gacgggcttg
     2281 ccgccggggc cggaggagga gtagtagcgg tggcgtcctt gccgccgatg cccagtttct
     2341 cgagtatatt gctgaagaat cccatggcga tctccttatt attttgtcct gtttagaagc
     2401 ttagacaata aaattgagta tagttagccc gcacttcacc gacagtcaaa ttagtcgacc
     2461 ctggcctcgc gcaaaggttt attctcttac ttgagataaa tccctgctcc tacgatcacc
     2521 gtttctccct ccggcaattt agctctcttg atatagctca gcttcttgga aggcgttttt
     2581 tcaccgggtt tgggccacat atattccacc caccctgagc ccttggtttt ggccgtctct
     2641 acgaactccc gcatgaacgc cttgccattg gcatccttca agtcgaggat actttttgtc
     2701 tcaagttccg ggttggccgg atgcataagg atgacaccgt tcatatcggc aatgaagacg
     2761 taattgtccc ctctgagcca ttcgctccct ttctttttga attccgagaa tgcggttttt
     2821 cctttgctct cggttaaggt tgccgccttg tcgacgacag cggcaattcc cttggcctcc
     2881 ttcgactgcg tagcttcggc agaaaaggct gggggaacaa ataggcatag aactacaaaa
     2941 aaatttagaa gcgccattac ggctggtctt ttcattagat gctccattcc ccttttatgc
     3001 agaacgttga ttcaccatcg agcgacttcg agccagtcgg ccgatgcgcg agtaaagttg
     3061 ttttcgtcac tctagcaaaa tagttcgccg accccagact taacattcta tacaaaaaaa
     3121 ggcattgcaa tcatcatcaa caattaacta aggagccgaa aggcgaaagc atgccgcgtc
     3181 gtacacgtgt gccgcggctc caaagctcag ccggattaag cagaacgacg cctgttctga
     3241 aaatagatca ccgctcgatg aggtaattcc gaagggcgag acatcttcag ctaatcgtgg
     3301 cgccggggtt ttgtatatcc gcgcattcta ccggagcaga gaaa
//
LOCUS       DASQKS010000083         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3825119, whole genome shotgun sequence.
ACCESSION   DASQKS010000083 DASQKS010000000
VERSION     DASQKS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825119"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="pgk"
                     /locus_tag="VE170_02220"
     CDS             complement(<1..385)
                     /gene="pgk"
                     /locus_tag="VE170_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYJ14272.1"
                     /translation="MKLASIDRFEPTELAGLPVLVRVDAADDAKLRNALPTLAHLAEA
                     RARIVIATHRDAAPDGAPAADRIAASLSEMLGRPVNSLDDWKGEAGLRAVSRLGDGGI
                     LMIENLAREPGESAGDDGLADALGRL"
     gene            complement(410..1543)
                     /locus_tag="VE170_02225"
     CDS             complement(410..1543)
                     /locus_tag="VE170_02225"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14273.1"
                     /translation="MISKADLEFLTERKAVENSPVLSVYLDTDQSKAVNLQRRFEASL
                     RDMLRSIEKRLDKPQLKNFAADAERVGEYVSGLEPRAKACLCFADDSEKFFWARTINA
                     PVHNQARWSDTPYVVPLLEIFDEHERYGVVLVDKAHARLVSVHIGEIEEHFDALAPLP
                     MRRVRATGTDHILSERRFQSKAQTHVHWHLKHVAESLDKLVDQYGIDRLLLAGPVEAT
                     GELQQLLSKRLRGRVVSSLSLPTKASLGEILEAVVKVEHGVERRLEKETVENLISGDG
                     RHPSVLGLERTVYALYEERVWRLIYAAGFNPPGGRCGHCGMLFTNTAGSCDYCGGTVN
                     RIDDLLEQMVERVLDQDAKVEQVTGDAALRLQQVGNIGAVLRF"
     gene            complement(1647..2096)
                     /gene="trxA"
                     /locus_tag="VE170_02230"
     CDS             complement(1647..2096)
                     /gene="trxA"
                     /locus_tag="VE170_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYJ14274.1"
                     /translation="MAEPLLIRCSHCGATNRVPPEKLALGLQPVCSRCKSPLPIGESK
                     PITVNDANFSTEVEGSPLPVLLDLWAPWCAPCRMVAPVLEELAKEMAGRVRVAKLNVD
                     ENPATANRFQARSIPTLLVLKGGKEIERIVGAQPKTEIVRVLQRAMA"
     gene            complement(2110..>2808)
                     /locus_tag="VE170_02235"
     CDS             complement(2110..>2808)
                     /locus_tag="VE170_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ14275.1"
                     /translation="TPQSEQNNRAVFFKSAIGQLDRLYEFVRHQLAYLESVGDLMPGE
                     LSAEDVADAAMLRASREFAKQPAEREVGSWLIDLAQQQLEREVKKLKAERNRTVHIEK
                     PIPETPPAEEVVTLGDEILDFYEPDQALKLEDVFPDVDVSTPEDFVAAKEELLQCINA
                     ALAAMPKEWSRALHLHHSGGLTLKELGEVLQKNQPEIERILEYARLHLRQSLIESGCT
                     FIVNGSQNKSRAGA"
BASE COUNT          519 a          847 c          834 g          608 t
ORIGIN      
        1 cgaggcggcc gagggcatcc gctaagccat catcgccggc agattcgccg ggttcacggg
       61 cgagattttc gatcatcaaa atgccgccgt cgccaagacg gctgacggcg cgcaggccgg
      121 cttcaccttt ccagtcgtcc aggctgttga ccggccgacc gagcatttca ctcaagctcg
      181 cggcaatacg gtccgccgca ggcgcgccat cgggcgccgc gtctcgatgg gtggcgatga
      241 caatacgcgc gcgcgcttct gccaaatgcg ccagcgtcgg cagcgcgttg cgaagtttgg
      301 cgtcgtctgc ggcatcgacg cggaccaaca ccggcaagcc ggccaattct gttggttcaa
      361 acctatcgat gcttgcaagt ttcatcgccg tccccaattg aatttatcgt caaaatcgca
      421 ataccgcgcc gatgttgccg acctgttgca gccggagggc ggcgtcgccg gtaacttgct
      481 cgaccttggc gtcttggtcg aggacccgtt cgaccatctg ttcgagcaaa tcatcgatgc
      541 ggttgaccgt tccgccgcag tagtcgcagg agcccgcggt gtttgtaaac aacatgccgc
      601 aatgaccaca gcggccaccc ggcgggttaa accccgccgc atagatcagc cgccaaaccc
      661 gttcttcgta aagcgcgtat accgtgcgct caaggccaag gacgctcggg tggcggccat
      721 cgccgctgat cagattttcg accgtttctt tttccaggcg ccgttcgacg ccatgttcga
      781 ctttgacaac ggcttcgaga atttcgccga ggctcgcctt ggtcggtaac gataggctgc
      841 tcacaacgcg gccgcgcaaa cgcttggaca agagctgctg gagctcgccg gtggcctcca
      901 ccggtccggc cagcaacaag cggtcaatgc catattgatc gaccagtttg tcgagggact
      961 cggcgacatg tttcagatgc caatgcacat gggtttgcgc tttgctctga aaccggcgtt
     1021 ccgacagaat atgatcggtg cccgtggctc tgacgcggcg catgggaagg ggcgccaggg
     1081 cgtcgaaatg ttcttcaatc tcgccgatat gaacgctcac cagccgggcg tgggctttat
     1141 cgacgagcac gactccgtaa cgctcatgct cgtcaaaaat ttccagcaac ggcacgacat
     1201 agggcgtgtc gctccagcgt gcctgattgt gcaccggggc gttgatcgtg cgcgcccaga
     1261 aaaacttctc cgaatcgtcg gcaaagcaaa ggcatgcttt cgcccgtggc tccaatcccg
     1321 aaacatattc gcccacgcgt tcggcgtcgg cagcgaagtt tttcagctgc ggtttgtcca
     1381 gtcgcttttc gatcgatcga agcatgtctc tgagggaagc ttcgaagcgg cgctggagat
     1441 tgacggcttt gctctggtcg gtgtcgaggt agacgctcag caccggactg ttctctactg
     1501 ctttgcgctc ggtcagaaat tccaggtcgg ctttggagat catgttatgg cgtttatcct
     1561 tttcaagccg ttcccctgcg atcgctggcg cggccgattg cacatcgggc ctcaggtgaa
     1621 aactgcgcga tgccgccgcg tcagggttac gccatggcgc gctgcaaaac gcgcacgatc
     1681 tcggttttcg gttgcgcgcc gacgatgcgt tcgatctcct tgccgccttt gagcacgagt
     1741 aacgtgggaa tgctgcgcgc ctggaagcgg ttcgcagtgg caggattttc gtcaacgttt
     1801 aatttggcca cgcgcacacg tccggccatt tctttggcca actcctcgag caccggcgcg
     1861 accatccggc agggggcgca ccagggcgcc cagagatcga gcagcaccgg cagcggcgag
     1921 ccctcgacct cggtggaaaa attcgcatcg ttgaccgtta tcggtttgct ttcgccgatg
     1981 ggcagaggcg atttgcagcg gctgcacacc ggttgcaagc cgagggcgag cttttccggc
     2041 ggcacgcggt tggtggcgcc gcaatgggaa cagcgaatca atagaggttc cgccatttta
     2101 tttccgccct tacgcgccag cgcgcgattt attttgactg ccgttgacga tgaacgtaca
     2161 acccgattca atcaggctct ggcgcagatg caggcgcgca tattcgagaa tgcgttcgat
     2221 ctccggctgg tttttctgca acacctcgcc aagctctttc agggttaacc cgccgctatg
     2281 atgcagatgc aaggctcggc tccattcctt cggcatcgcc gccagcgccg cgttgatgca
     2341 ctgcagcaat tcttctttgg cggcaacgaa atcctccgga gtcgaaacgt cgacatccgg
     2401 gaacacatct tcgagcttga gcgcctggtc gggctcgtag aaatcgagaa tctcgtcgcc
     2461 gagagtcacc acctcttcgg ctggcggcgt ctcgggaatg ggcttttcga tgtggacggt
     2521 gcggttgcgc tcagccttca attttttgac ttcgcgctcg agctgctgtt gggcgagatc
     2581 gatcagccaa ctgccgacct cgcgctcggc gggttgtttg gcgaattcgc gggaggcccg
     2641 cagcatcgcc gcgtccgcca cgtcttccgc gctgagctcg ccgggcatca gatctcccac
     2701 ggattccaga taggcgagct ggtggcggac gaattcatag agccggtcca gttgcccgat
     2761 agccgactta aaaaatactg cgcgattatt ttgctctgac tgaggggt
//
LOCUS       DASQKS010000084         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_889324, whole genome shotgun sequence.
ACCESSION   DASQKS010000084 DASQKS010000000
VERSION     DASQKS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889324"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VE170_02240"
     CDS             complement(<1..1139)
                     /locus_tag="VE170_02240"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HYJ14276.1"
                     /translation="MSNVTGTVPANSHIQNWVKGVASMCQPDNVYWCDGSEQEREQLT
                     KIAVQCGDLLPLNQKEEPGCYLHRSALNDVARTENLTFVCTDQQEEAGPNNNWMAPAE
                     AYDKLGKIFAGSMRGRTLYVIPFLMGPAGSSFSKVGVQVTDSVYVVLNMRLMTRMGKI
                     ALDHLGNSDDFTRCLHSKADLDIERRFICHFPQDNTVWSVGSGYGGNALLAKKCLSLR
                     IASKLGQQEGWMAEHMLIVGVESPDGEITYVCGAFPSACGKTNMAMLVPPPSMKGWKI
                     HTVGDDISWLRVGSDGRLWAINPEAGFFGVAPGTGSNTNPNAVKTVKKDTIFTNVALR
                     DDGTVWWEGHDDPVPANAQDWRGKPWDPEGGSPAAHPNARFTVSI"
     gene            complement(1302..2204)
                     /gene="truB"
                     /locus_tag="VE170_02245"
     CDS             complement(1302..2204)
                     /gene="truB"
                     /locus_tag="VE170_02245"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYJ14277.1"
                     /translation="MTFTRSGLLLIDKPTGPSSAHVVHRVKKILGAKRVGHLGTLDPF
                     ASGLLPIGINEGTKIADIFLGGAKSYRAVMVLGIATDSQDATGKIIDERPVPALVDEE
                     LRRLEAKFSGAQTQVPPMFSALKKDGVRLYELARQGQEIERAPRSIQIDRLELRRRSD
                     NEIEFEVTCSRGTYVRTLAADMGAALGCGAHLLQLRRLSCDHLSLTQATNVDELEGTV
                     AGGNVPLMTLANALSHFPAVTWDNRSIARLRQGQQEILLQLKLPADGASLLSILDQAG
                     ELVALVQWAVDPSGGRWRLYRVFH"
     gene            complement(2290..2640)
                     /gene="rbfA"
                     /locus_tag="VE170_02250"
     CDS             complement(2290..2640)
                     /gene="rbfA"
                     /locus_tag="VE170_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYJ14278.1"
                     /translation="MEYQRSERVGDLLLEVTAELLRREIRDPRLRDVALTAVKMSKDL
                     RHARIYFNLLGGSANHDDATAGLKSASGFIRTKAAKQLNLRFVPNIEFTYDESADEAQ
                     RIDALLKQASKTEE"
     gene            complement(2792..3079)
                     /locus_tag="VE170_02255"
     CDS             complement(2792..3079)
                     /locus_tag="VE170_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HYJ14279.1"
                     /translation="MVVGILKLQLAIPENHSLKGKRGVVKRIQARVTQRFNVSVTECG
                     DQDLWQSSLLGFAIAGTSRPIVEASLRKIVDFVDALGLAEVGEAGVEFFHC"
     gene            complement(3124..>3729)
                     /locus_tag="VE170_02260"
     CDS             complement(3124..>3729)
                     /locus_tag="VE170_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HYJ14280.1"
                     /translation="SVEAVSEALTRMSTGDVKLKVLHGSVGGITESDILLAAASNAIV
                     IGFNVRPESKGAALAAKEGVDVRLYTIIYEVVADVRAAMEGLLEPTFREQVFGRVEIR
                     QIFNIAGIGTIAGCSVTEGKIARGNLVRLLRDQVVVHEGKLASLKRFKDDVREVASGY
                     ECGLSLEGFQDLKQGDVVEAFERIPVIRRIAPVQTGREARV"
BASE COUNT          793 a         1119 c         1034 g          783 t
ORIGIN      
        1 ttgatcgaca cggtgaagcg cgcgttggga tgtgccgccg ggctgccacc ttcgggatcc
       61 cagggcttgc cgcgccaatc ctgagcgttg gctggcaccg gatcgtcgtg gccttcccac
      121 cacaccgtgc cgtcatcgcg cagcgctacg ttggtaaaga tggtgtcttt cttaaccgtc
      181 ttcacggcgt tgggattggt attcgatccc gtgccagggg caacgccgaa aaaacccgct
      241 tccggattga tcgcccagag tctgccgtcg gagccaacgc gcaaccagga gatgtcgtcg
      301 ccgacggtgt gaattttcca acccttcatg ctcggcggcg gcaccaacat cgccatattg
      361 gtcttaccgc aggcgctcgg aaaagcgccg cagacatagg taatctcgcc gtcggggctt
      421 tccacgccga cgatcagcat atgctcggcc atccagcctt cttgttggcc taatttgctg
      481 gcgatgcgca gcgacaaaca ttttttcgcc agtaaggcgt taccgccgta tcccgagccg
      541 acgctccaaa ccgtgttgtc ttgaggaaaa tggcaaatga aacggcgctc gatatcgaga
      601 tccgctttgg aatggaggca gcgggtaaag tcgtccgagt tgccgagatg atccaaagca
      661 attttgccca tgcgcgtcat cagccgcatg ttgagcacca catagacgct gtcggtcacc
      721 tgaacgccga ctttactaaa gctcgatccc gccggtccca tgaggaaagg gatgacgtaa
      781 agcgtccgcc cgcgcatcga gccggcgaag attttgccga gcttgtcgta ggcctccgcc
      841 ggcgccatcc aattattgtt aggaccggcc tcttcctgtt ggtcagtaca aacgaaagtc
      901 aggttttcag tccgcgccac atcgttgagc gcgctgcggt gcagataaca gcccggttct
      961 tctttctgat tgagcggcaa cagatcaccg cactgcacgg cgattttggt cagctgctcg
     1021 cgctcttgtt ccgaaccatc gcaccaatag acgttatcgg gctgacacat cgatgcgacg
     1081 cccttaaccc aattttgaat atgtgaattt gccggcaccg ttcctgtaac gttactcatg
     1141 agtgatagat gcttcctttc ctaaaacact gcgaggttgt gaatcagcat cccacggcca
     1201 tccaagatgg tgatatcgtt aacgttcgat aggactaata agttatctga ttggcccggt
     1261 tgatgtcaat aaaccggatt cgtggcgcgc gcgccagaac tttaatgaaa aacccggtac
     1321 aggcgccaac ggccgccgga cgggtctacc gcccattgca ccagtgcaac cagctcgccg
     1381 gcttgatcga gaatcgacag tagagatgcg ccgtcggctg gcaactttaa ttgcaataat
     1441 atttcctgct gcccctgacg caaccgcgcg atcgaccggt tgtcccaagt gaccgcgggg
     1501 aaatggctta aggcgttggc gagtgtcatc aacggcacgt taccgccagc caccgtgcct
     1561 tccagctcgt caacgttggt cgcctgggtc aaagacagat ggtcgcagct caagcgccgg
     1621 agctggagca aatgggcgcc gcaacccaac gccgcgccca tatccgcggc caaagtccgc
     1681 acgtaggtgc ctcgcgaaca agtcacctcg aactcgattt cgttatcgct gcgacggcgc
     1741 aactcgagcc gatcgatctg tatcgagcgc ggcgcgcgct ctatttcttg cccttgtctg
     1801 gcgagttcat acaaacgcac gccgtctttt ttcaacgctg agaacatcgg cggcacttgg
     1861 gtttgcgcgc cggagaattt cgcttcgagc cggcgcaact cttcatccac cagcgccggc
     1921 accggccgct catcgattat tttaccggtg gcgtcttggc tgtccgtggc tatccccaag
     1981 accattaccg cgcggtagct cttggcgccg ccgagaaaaa tatctgcaat cttggtgccc
     2041 tcgttgatgc cgatcggcag caagcccgaa gcgaacggat caagagtacc caaatggccg
     2101 acccgtttag cgccgagaat ttttttgacg cgatggacga cgtgggccga tgaaggaccc
     2161 gtcggcttat cgatcaacaa cagaccgctt cgggtaaaag tcataagctc gcggcgaccg
     2221 gctgtaacaa gaaatttcgc ggcaaaagcg cgcccggcag cgcagactaa ttattgcccg
     2281 ggcgcgaaac tactcttcag tcttgctggc ctgcttcaac agcgcgtcga tacgctgcgc
     2341 ttcgtcggcg gactcgtcgt aggtaaactc gatgttcgga acgaaacgga gattcagttg
     2401 cttggctgct ttggtccgaa tgaagccgct ggcgcttttg agccccgctg tcgcatcgtc
     2461 atgatttgcc gaaccgccga gcagattgaa gtagatgcgc gcatggcgca gatccttcga
     2521 catcttgacc gccgtcaaag cgacgtcgcg gaggcgcggg tcacggatct cgcgccgcaa
     2581 gagttccgcc gtcacctcca acagcagatc cccgacccgc tctgaacgtt ggtattccat
     2641 ggttttggtg tgaaaaaaac tattttcacc gtgctagcaa ggcgggcaca atggatcagg
     2701 agaaatgatt atcaactttg cactcttgac acctgcgccg cagacgaatc tttctgtatt
     2761 gccgcgaatc gactcatgat cgggaactat ctcaacagtg aaagaattcg acaccggctt
     2821 cgccgacttc ggctaatccc agcgcatcga cgaagtcgac gatcttccgc aatgaggctt
     2881 cgacgatggg ccgactggtg ccggctatgg cgaagccaag aagcgagctc tgccagagat
     2941 cctgatcgcc gcattcggtg accgacacgt tgaagcgctg ggtgacccgc gcctggatac
     3001 gtttcacgac gccgcgcttg cccttcaaag aatgattttc agggatcgcc agctgcaact
     3061 tcaggattcc cacgaccatg aaagaacagc ggcggcacta acggcgaatg cggttaggac
     3121 cgcttaaacc cgcgcctcgc gaccggtttg caccggagcg atgcgtcgga ttaccggaat
     3181 ccgttcgaac gcctcgacca catcgccctg cttgagatcc tgaaaccctt caagggaaag
     3241 accgcactca tagcccgagg cgacttcgcg gacatcgtct ttgaagcgct tgagactagc
     3301 gagtttacct tcgtgcacca ccacttggtc gcgcagcagc cggacgagat tgccacgggc
     3361 gatcttgccc tcggtgaccg agcaaccggc aatggtgccg atgccggcaa tgttgaagat
     3421 ctggcggatt tcgacgcggc cgaaaacctg ctcccggaac gtcggctcca acaaaccttc
     3481 catggcggcg cgcacatccg ccaccacttc gtagatgatt gtgtagaggc gcacgtcgac
     3541 gccttctttg gccgccaacg ccgcgccttt ggattccggc cgcacgttga agccgatgac
     3601 gatagcgttg gaagcggcag cgaggaggat atcgctttcg gtaatcccgc ccaccgagcc
     3661 gtgcaggact ttcagtttga cgtcgccggt ggacatgcgc gtgagcgctt cagacaccgc
     3721 ctcgacgga
//
LOCUS       DASQKS010000085         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2985358, whole genome shotgun sequence.
ACCESSION   DASQKS010000085 DASQKS010000000
VERSION     DASQKS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985358"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VE170_02265"
     CDS             complement(<1..878)
                     /locus_tag="VE170_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14281.1"
                     /translation="MRAADALVDILRTEGVKFISTLPGDDILPIFDALHENGRVKLIL
                     TRHEQAAVYIADGYARSTGEVGVAMVTKGPGRCNAFTAIVNAFTDCVPLVVIFGHSSR
                     QQLAKGMLQEVPYLDTFQTMAKWTFSVPSAERIPEVFRRAFTLARSGRPGPVIIEVPQ
                     DISMAQAEIPPYYKTRRVRFGPDPDDVDKLIELLRGAERPLIYVGRGALWSGATDALI
                     QVAESYALPVMATLPAKGAMPEDHPLCLGLGGYPRAVYSTGQSRKFAEEADLVIALGC
                     SFRQHATSNWLPKPAHT"
     gene            complement(891..1430)
                     /locus_tag="VE170_02270"
     CDS             complement(891..1430)
                     /locus_tag="VE170_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14282.1"
                     /translation="MNRIVTHILLWIVIWSLVVMAQPLGAAQPKKLRLAFSAFAYAIP
                     PFWIAHELKLFEKYGGYDTELVYVGGSRPIQAMLGGSTTWEAIYSYQTRPELTVPVVA
                     KYMRVAKDDPAVVEAQRTIGQMLNQNLPPCLDGVRFVLDHYAERQPSLVHKNLADFVD
                     MRFVRKLEEEGFFKKFASK"
     gene            complement(1640..2488)
                     /locus_tag="VE170_02275"
     CDS             complement(1640..2488)
                     /locus_tag="VE170_02275"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYJ14283.1"
                     /translation="MESPSRGSLQGLTVVCFESRRAKEMAELIRRYDGAPIVAPSMRE
                     VALSENRAALNLLPQIEAGGFEMLILMTGVGTKTLNEILLSQFPQERIIAALKKVQLI
                     ARGPKPIGALKDLGLQASLTVPEPNTWREILATLDTAIELRGKRIAIQEYGIANPELS
                     AALEQRGAMVTAIPIYRWALPEDLAPLRDAIVKILHGEVDVALFTNGAQVDHIFQIGG
                     FDKATEDLRQGFNKIVIGSVGPVCTQVLRRFGIEPDIEPVHPKMGSLIAEGAARARDV
                     LAKKSP"
     gene            complement(2496..>3001)
                     /locus_tag="VE170_02280"
     CDS             complement(2496..>3001)
                     /locus_tag="VE170_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14284.1"
                     /translation="TDTIAGWVKDRAVGLIPRNYRQGDMGDFSIAFVATDDGAVNAQA
                     FSDALRLGVWMNAADDPAHCDFILPSVLRRGDLTVAVSSGGTSPALSRTIREELELYF
                     TEEYALLVKLAAEARDELHERGLSVPFETWRRALSGDVRQFLMRGEIARAKVLLLREL
                     GVEVYVE"
BASE COUNT          629 a          907 c          873 g          592 t
ORIGIN      
        1 gtatgggccg gcttgggcag ccagttggaa gtcgcgtgct ggcggaaact gcagccgagc
       61 gcgatgacga gatcggcctc ttcggcgaac ttacgcgact gcccggtgct gtagaccgcg
      121 cgcggatacc cgccgaggcc gagacagagg ggatgatctt ccggcatcgc gcccttcgcc
      181 ggcaaggttg ccatcaccgg taaggcatag ctctcggcga cttgaatcaa agcatcggtg
      241 gcgcccgacc atagcgcgcc gcggccgacg tagatcagcg gccgctcggc gccccgcaac
      301 agctcgatca gcttatcgac atcgtcggga tcgggaccga agcgcacgcg ccgggtcttg
      361 taatagggcg ggatttccgc ctgggccatg ctgatatcct gcggcacttc gatgatcacc
      421 ggtcccggcc tgccggaacg cgccagcgtg aacgcccggc gaaacacttc gggaattctc
      481 tccgccgacg gcacgctgaa cgtccacttc gccatggtct gaaaggtgtc gaggtacggc
      541 acttcctgca gcatgccttt ggccaattgc tggcgcgaac tatgaccgaa gatgaccacc
      601 aacggcacgc agtcggtgaa tgcattaacg atggcggtaa acgcattgca gcggcccggt
      661 cccttggtca ccatcgccac gccgacctcg ccggtgctgc gcgcgtagcc gtcggcaata
      721 tagaccgccg cctgctcatg ccgggtcaga atcaatttca cccggccgtt ttcgtggagc
      781 gcgtcgaaga ttggcaaaat gtcatcgccg ggcaacgtcg aaatgaactt caccccttcg
      841 gttctgagaa tatcgaccag tgcatcggca gcgcgcatgt tttgatcttt ctatttgctg
      901 gcaaattttt tgaagaagcc ctcttcttcg agcttgcgaa cgaaccgcat atcgacgaaa
      961 tcggcaagat ttttatggac taaactgggc tggcgctcgg cataatggtc gagcacgaag
     1021 cgcacgccgt cgaggcatgg cggcagattt tggttcaaca tttggccgat ggtgcgctgc
     1081 gcttcgacca ccgccggatc gtctttggca acgcgcatat acttggccac caccggcacg
     1141 gtcagctctg gccgggtctg atacgaataa atcgcttccc acgtcgtgga gccaccgagc
     1201 atcgcctgga tcggccgcga accgccgaca taaaccaatt cggtgtcata gccgccatat
     1261 ttctcgaata gttttagttc atgagcgatc cagaacggcg gaatggcgta agcgaaagcg
     1321 ctaaaggcga ggcgcagctt ctttggctgc gcggcgccga gcggctgcgc catgacaacc
     1381 agcgaccaaa tcactatcca tagaaggatg tgagtaacga ttcgattcat tgaatgcgcc
     1441 gctcggatga agcctgagac atagtggcaa acgattcggc agtcaaggcg aagttcccac
     1501 actgaacccg tcgtgagccg caaagcagat tgccttgcca tccgggcgat ccaagatccg
     1561 atcgtcccca ggttgtcgca ggtgaaaacc gattgcacga ttcgaccggg cggtaacgca
     1621 cagaatggaa agagaatgtt tacggtgatt tttttgccag cacgtcccgc gccctagccg
     1681 cgccctcggc gatcaacgaa cccatcttcg gatgcaccgg ctcgatgtcg ggttcgatgc
     1741 cgaatcggcg caggacctga gtgcacaccg ggccaacgga gccgatgacg attttattaa
     1801 agccttggcg caaatcctcg gtggctttat cgaagccgcc gatctgaaaa atatgatcga
     1861 cctgggcgcc gttggtgaac agcgccacat cgacttcacc atgaagaatc ttcacaatgg
     1921 cgtcgcgcag cggcgctaag tcctctggca atgcccagcg atagatcgga atcgcagtga
     1981 ccatggcgcc gcgttgctcc aacgcggcgc tgagctcggg attggcgatg ccgtattctt
     2041 gtatcgcgat acgcttgccg cgcaattcga tagcagtgtc taaggtcgcg agaatttccc
     2101 tccaggtatt gggctcgggc acggtcagcg acgcttgcag tcccaaatcc tttaacgcgc
     2161 cgatcggctt gggaccgcgc gcgatcaatt ggactttctt caaggcggca ataattcgct
     2221 cctggggaaa ctggctgagg agaatctcat tgagcgtctt ggtgccgacg ccggtcatga
     2281 ggataagcat ctcgaagccg cccgcttcga tttgcggcag taaattgagt gcggcgcgat
     2341 tttcgctgag cgccacttcc cgcatggacg gcgcgacaat cggcgcgccg tcataacggc
     2401 ggatgagttc cgccatctct ttggcgcgcc ggctctcgaa gcagacgacc gtgagccctt
     2461 ggagactccc ccggcttggt gattccatag aacgcttatt ccacgtacac ttctacgccc
     2521 aattcgcgca gcaacaatac tttagcgcgc gcgatttcac cgcgcatcaa aaattggcgc
     2581 acgtcgccgc tcaaggcgcg ccgccaggtt tcgaagggca cgcttagccc gcgctcatgc
     2641 agctcgtccc gcgcctcagc cgccaatttt accaataagg catattcctc ggtgaagtag
     2701 agttcgagtt cttcccgaat ggtgcgcgac agcgccgggc tggtaccgcc gctcgacacc
     2761 gcgacggtga gatcgccgcg gcgcaacacc gacggcaaga tgaaatcaca atgcgccgga
     2821 tcgtctgccg cgttcatcca cacgcccaac cgcagagcgt cactgaacgc ttgcgcgttg
     2881 acggcgccgt cgtcggtggc gacaaaggca atggaaaaat cgcccatgtc gccctgccgg
     2941 tagttgcggg gaatcaggcc gacggcacga tctttcaccc agcccgcaat ggtatcggtg
     3001 a
//
LOCUS       DASQKS010000086         9214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5506534, whole genome shotgun sequence.
ACCESSION   DASQKS010000086 DASQKS010000000
VERSION     DASQKS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5506534"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VE170_02285"
     CDS             <1..441
                     /locus_tag="VE170_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ14285.1"
                     /translation="GGKLVFGGKRPTGDEFDKGFYLQPTIVADVDPASRMIQEEVFGP
                     ALPIVRVKTLDQAIEHANGSIFGLGSSIWTRDINRAMYGAENIEAGYTWVNSAQIIYD
                     ELPFGGVKQSGLGKEHGDEAIEYYTDSKSVVIATDVGGEIIGGE"
     gene            520..2064
                     /locus_tag="VE170_02290"
     CDS             520..2064
                     /locus_tag="VE170_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HYJ14286.1"
                     /translation="MNVELPEIYNAATTFVDENITQGRGGKVAIYYQDRHITYQEVFE
                     NVNRTGSALRDLGIEIEQRVLVILPDSPEFAYSFFGAIKIGAVAVPTNPWMFAKDYEY
                     LINDSRARAIIVHESTLPEIEKIWDNTPFLKHIIVVGTPNGKALSYENLTAQASSKLD
                     AEKTSRDDVCFWCYTSGSTGNPKGAVHLQHDMVTISDLFVAPVLQMKETDLCFSASKM
                     FFSYGLGNSLYFPFRFGASTVLWPDKPEPEKILQVIEKYKPTFFFSVPTLFARFLRID
                     KKYDVSSLRTCLSSGEPLPPALFHQWKERTGLELLDVVGSTEATHDFLANRPGRAKAG
                     SSGEVTPAFEAKIVDDEGRDLPIGEIGNLMVKGDATAPFYWNKHEQTKRMMQGEWLKT
                     GDTYYRDAEGYYWYCGRSDDMMKVGGLWVSPIEIENTLMEHPAVLESGVVCAQDADGL
                     LKPKAYILLKNEYKASDALKVELQNHVKSKLQPYKYPRWVEFVDDLPKTVTGKIQRFR
                     LRANEN"
     gene            2122..2832
                     /locus_tag="VE170_02295"
     CDS             2122..2832
                     /locus_tag="VE170_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ14287.1"
                     /translation="MAVNIKELKENLAYSCNILANEGHWDNILGHVSVRVPGQNKILM
                     KPHSFGFEEIRPQHIIVVDIASGKKTEGKYERHSEVFIHTEIMKARKDVNCVVHSHPP
                     YATAFGALGQKLRPISHEGNIFYEGLPTFDYTTALIRTPELGVEVAKSLGNCRALLMK
                     NHGSTVVGPTIEVATLYAVFLEKAARIQLLATASGDPSWTSDAEAAVKYEQIYTPHRL
                     DSMWEYYVRRAKKFRKSS"
     gene            complement(2943..3935)
                     /locus_tag="VE170_02300"
     CDS             complement(2943..3935)
                     /locus_tag="VE170_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14288.1"
                     /translation="MLKGFLKIAVLLWSLTLSTQAGAQVPVRINWTAVTGAQSGMFMA
                     QQEGLFKKNGLDVELLHISSSSRGIQAILAGEIAFSYMDGSNQVQANLKGANLAFVAG
                     ATNRQVFSLMAKPEIKRISDLRGKKIGITRIGSSTHTSALYALNTAGLKQNDYQILPL
                     VEVPNILTALIAGQVDAGVVSPPTNSRARKAGLNELMNLAKEGPEFVSVAIGTSRSYI
                     KANEDTVRRVVRSYAEGVQMFKANKAAAQRMMQKYLRVKEQDILDDAYNQFREYLEIP
                     PYVTRKAMENVIADVAVSDATAKSSKPDDFMDMRFVAELDKQGFFKATGSKGSK"
     gene            4044..5009
                     /locus_tag="VE170_02305"
     CDS             4044..5009
                     /locus_tag="VE170_02305"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14289.1"
                     /translation="MSKKVHLTLAVGDYESVRALKEGTVKADGIELTVITDMTSSMRH
                     WRMLRNREFDCAELSMSNYLMAKYTDLPFVAIPVFLHRRFRHGFVFLNASKGITSPSD
                     LIGRKVGLRNFQATANLWIRGILEHEYGVPHRSLIWLKQDDEEVDWTPPADLKIQRIP
                     AGKNIDKMLVDGEIDALIHPELIQPILDKDPRVSRLFPDYRDLELHYFKRTGIFPIMH
                     TTAIAQKIVDKYPWVPLNLYQALEASKKAAYKRMENPRIVPLAWFRSFLEEQEELLGP
                     DPWVNGLGDVNRKALETLMQYSQEQGLIGRKMTLDELFISCEAHS"
     gene            5306..5668
                     /locus_tag="VE170_02310"
     CDS             5306..5668
                     /locus_tag="VE170_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14290.1"
                     /translation="MFGKGVFQAEENLLREIVRSIDKKIDYSAREGEGSKFTLHLKFK
                     NHEGDVVLDLEDLKNAKQDQIRRHQIRQKIKARCDHLDTSRYGADILGLRSARILKTA
                     PKLVPLAQRSFNRNAPRR"
     gene            5723..6586
                     /locus_tag="VE170_02315"
     CDS             5723..6586
                     /locus_tag="VE170_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HYJ14291.1"
                     /translation="MAKAELIRQALATRGQLIMPGVYDALSAKIAARAGFEVTFITGY
                     SLSATLLGEPDFGLLTQTEVVGAAQRICSVIDIPVIVDADTGYGNAINVTRTVQDLIR
                     AGAGGMFLEDQVWPKRCGHMRGKQVIPLEEQLNKLRAAVEAKGQNDFFIVARTDSRQA
                     HGLDEAITRGIAFSAAGADAVFIEAPESKEEMREIAKHVKGHLVANMLERGVTPLMRP
                     NELKELGFDLVVWPLAPLYSVAKSLTDVYTTLRKEGSTLSILDRLMPFDEFNDIVGLN
                     EKYLLDAKFKT"
     gene            complement(6623..7729)
                     /gene="dnaN"
                     /locus_tag="VE170_02320"
     CDS             complement(6623..7729)
                     /gene="dnaN"
                     /locus_tag="VE170_02320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYJ14292.1"
                     /translation="MEIKAKRGDLLATLYWTQSIVERRNTMPILANVMIEALRSGIRI
                     TATDLEVGVRGGVDGEIIKEGSVTVNAKKLYEIVREVPNDLVQLKRLDNDWVEIRSGK
                     SVFKIFGMDAKEFPQFPQFDSSGLSTTPAATVREMIERTIFSVSTDETRYSLNGVYIE
                     QSEGGKVRMVATDGHRLAFEEKHVGSLGLTKGVILPRKGLSELKKLLESGEDGAVSLG
                     FKENMGLVSKDKVELFMRLIDGDFPDYTKVIPVGNPNIAKLDHEELLRALRRVSILSS
                     ERYKGVKMEFANGKLSISANNPDLGEAVEEVEADYKGKTIAIGFNARYLLDVLAVLGG
                     EGDIEFELKDELSPSVIRKAGVDGYLYVLMPMRL"
     gene            complement(7957..9174)
                     /gene="dnaA"
                     /locus_tag="VE170_02325"
     CDS             complement(7957..9174)
                     /gene="dnaA"
                     /locus_tag="VE170_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYJ14293.1"
                     /translation="MAVANKFFRDWVGQNYLADLESAVSAVAKHDLKVVIEVERNGDQ
                     SNPQEELQRKDDKERERQPRQCYLARKYTFDNFVIGTSNQFAHAACMAVANQPGEHYN
                     PLFIYGGVGLGKTHLVNALGHQAAAKRPNLKVVYLSSESFMNELISSLRRDKMDEFKK
                     KFRNVDILIIDDIQFIAGKERTQEEFFHTFNSLYESHKQIVITSDKFPKDIPAIEDRL
                     RNRFEWGLIADIQPPDMETRVAILQKKAEFEGVPLPGDVAIFLASNISSNVRELEGSL
                     TRLGAFYSLTKAAITIDFAKDVLRNNLKSYEREITVEGIQKSICDHYNLKISDLKAHR
                     RTQNLAFPRQVAMYLCRKHTQTSFPGIGDKFGGRDHSTVIHASKTIERRIRDDPNMQA
                     TIAMIERNLNIHK"
BASE COUNT         2240 a         2452 c         2309 g         2213 t
ORIGIN      
        1 ggtggcaaac tggtcttcgg cggcaaacgt cccaccggcg atgagtttga caaaggtttt
       61 tatctacagc cgaccatcgt cgccgatgtc gatccggcct cgcgcatgat ccaggaagaa
      121 gtcttcggcc cggctctgcc gatcgtacgg gtaaaaactc ttgaccaagc catcgagcac
      181 gccaacggct cgatcttcgg tctcggttcg tcgatttgga cgcgcgatat caatcgcgcc
      241 atgtacggtg ccgaaaatat cgaagccggg tacacctggg tcaactcggc gcagattatc
      301 tacgacgaac tgccttttgg tggcgtcaaa cagagcggtc tgggcaaaga acatggcgat
      361 gaagcgatcg agtactacac tgattcgaaa tcagtcgtca tcgccaccga tgttggcggg
      421 gagattatcg gcggcgaata ggccgctacg cggcattcca gattaaatat tccagatgcc
      481 aaatctggaa tatgaaattt ggaatttgga attgcctcca tgaacgtgga actgcccgaa
      541 atctacaacg ccgccacgac cttcgtcgat gaaaacatca cgcaaggccg cggcggcaag
      601 gttgcgatct actatcaaga tcgccacatc acctaccaag aagtttttga aaacgtcaat
      661 cgcaccggca gcgcattgag agatctcggc atcgagatcg agcagcgcgt gctggtgatc
      721 ttgccggact cgccggagtt cgcctacagc tttttcggcg cgattaagat cggcgccgtt
      781 gcggtaccga ccaatccttg gatgtttgcc aaggattacg aatatttgat caacgacagc
      841 cgcgcccgcg ccatcatcgt ccatgaatcg accctacccg agatcgaaaa gatctgggac
      901 aacacgcctt tcttgaaaca cattattgtc gtcggcacgc ccaacggaaa agcgctgtcc
      961 tatgaaaatt tgaccgccca agcatcgagt aaactcgatg ccgagaaaac ttccagagac
     1021 gatgtctgct tctggtgcta cacctcgggc agcaccggca acccaaaggg cgccgtccat
     1081 ttgcagcatg acatggtcac gatcagcgat ctgtttgtcg cgccggtgct gcagatgaag
     1141 gaaaccgacc tgtgcttttc ggcgtcaaaa atgttttttt cctacggcct gggcaattca
     1201 ctgtatttcc ccttccgttt tggcgcgtcc accgtgctct ggccggacaa gccggaaccg
     1261 gaaaaaattc tccaagtcat cgaaaagtat aagccgacgt tctttttctc cgtgccgacg
     1321 ctgttcgcgc gcttcctgcg gatcgataag aaatacgatg tgagttcatt gcgtacatgc
     1381 ctgtcttccg gcgaaccatt gccgccggcg ctgtttcatc aatggaaaga gcgcaccggc
     1441 ctggaattgc tcgacgtcgt cggctccacc gaggcgactc atgatttttt ggccaatcgg
     1501 cctgggcggg cgaaagccgg cagcagcggc gaggtaacgc cggctttcga agccaaaatt
     1561 gtcgacgacg aaggacgaga tctgcccatc ggtgagattg gcaacttgat ggtcaaaggc
     1621 gacgccaccg caccgttcta ctggaacaaa catgagcaga ccaaaagaat gatgcagggc
     1681 gagtggctca aaaccggcga cacctactac cgcgacgccg aaggttacta ctggtactgc
     1741 ggccgctccg acgacatgat gaaagtcggc ggcctgtggg tttcaccgat tgaaattgaa
     1801 aatactctga tggaacaccc ggcggtgctc gaatccggtg tggtctgcgc tcaagatgcc
     1861 gacggtttac tcaagcccaa agcctatatt ctgctcaaaa acgagtataa ggccagcgat
     1921 gcactcaaag tcgagttaca gaatcacgtc aagagtaaac tgcagcccta caaatatccc
     1981 cgctgggtgg aattcgtcga cgacctgccg aaaaccgtga caggcaagat tcaaagattc
     2041 cgcttgcgag ctaacgaaaa ctgaaactaa gtccgcgatt cttctacctc ggtcctcgac
     2101 gatctctgcc aaggagtgaa aatggccgtt aatatcaaag agctcaaaga aaatcttgct
     2161 tattcttgca acatcctcgc caacgaaggc cactgggata atatcctcgg ccatgtctcc
     2221 gtaagagtgc cggggcaaaa taagattctc atgaagcccc atagcttcgg tttcgaagag
     2281 atccgtcccc agcatatcat cgtcgtcgac attgcgtccg gtaagaagac cgagggcaaa
     2341 tacgagcgcc actccgaagt gtttattcac accgaaatca tgaaggcgcg caaggatgtc
     2401 aattgcgtgg tgcattccca tccgccctac gcgacggcat tcggcgctct cggccaaaaa
     2461 ctccggccga tcagccatga aggtaatatt ttctacgaag ggttgccgac cttcgactac
     2521 accacagcct taatccggac tccggagttg ggcgtggaag tagcgaagag cctcggcaac
     2581 tgccgcgcct tgctgatgaa aaaccacggc tcgacggtgg ttggcccgac gattgaagta
     2641 gcgaccctct acgccgtctt tttggaaaaa gccgcgcgca ttcaactctt ggccaccgct
     2701 tccggcgatc cgtcttggac tagcgacgcc gaagcagcgg tcaaatacga gcagatctat
     2761 acgccgcacc ggctggattc catgtgggaa tactacgtgc gccgggctaa aaaattccgc
     2821 aagtcgagct agttcgatta cctgacatcg aagaggatat cgtcacttcg ttcacggtga
     2881 caaaggcata ttttccttct gagcgcgcga agaaccgcgt cctagctttc gctcaacgct
     2941 acctacttgc tacccttgct accggtcgcc ttgaagaagc cttgcttgtc caactccgcg
     3001 acgaaacgca tatccatgaa atcgtccggc ttggagctct tcgccgtcgc atcgctgact
     3061 gcgacatcgg cgatgacatt ttccatcgct ttgcgcgtaa catagggcgg aatttccaaa
     3121 tattcgcgga actgattgta ggcatcgtcc aaaatgtctt gctcttttac ccgcagatac
     3181 ttctgcatca tgcgttgcgc cgcggccttg ttagccttaa acatctgcac tccttcggca
     3241 taggagcgaa ccacccggcg caccgtgtct tcattggctt tgatatagct gcggctggtg
     3301 ccgatagcca ccgagacaaa ttccggcccc tctttggcta agttcatgag ttcgttaagc
     3361 ccggctttac gcgcccggct attggtcggc ggcgaaacca cgcccgcgtc gacctgtcca
     3421 gcgatcaatg cggtgagaat attcggcacc tccaccaacg gcaggatctg atagtcattc
     3481 tgcttcaaac cagcggtatt caatgcatac aaagccgacg tatgggtcga cgaaccaatc
     3541 cgggtgatgc cgatcttctt gccgcgcaga tcgctaattc ttttgatttc cggtttggcc
     3601 atcagcgaaa aaacctgccg attggtcgcg ccggcgacga aagcgagatt ggcgcctttc
     3661 aggttcgctt ggacctggtt cgacccatcc atataggaaa aggcgatctc gccagcgagg
     3721 atcgcttgga tgccgcgcga gctcgacgat atatgcagca attcgacatc caagccattt
     3781 ttcttgaata gcccttcctg ctgcgccatg aacatgccgc tttgagcccc ggtaaccgct
     3841 gtccaattga tccgcaccgg tacttgcgcc ccggcctgag tcgataaggt caaactccac
     3901 agcagcacag caatctttaa aaaccctttt agcatcgcgt ctccttgttc ttctcagacc
     3961 gataaaagtg ctatcacggt agcaattcaa cctgttaatt caaccgattg gcatgaccgg
     4021 tgaagcatgt tagataaccg cccatgagca agaaagtgca tctcacgcta gccgtgggcg
     4081 actatgaaag tgtccgcgcg ctcaaagagg gcacggtcaa agccgatggt atcgagctta
     4141 ccgtaatcac cgatatgaca tcgagcatgc gccattggcg catgctgcgc aatcgcgagt
     4201 tcgattgcgc cgagctatcc atgtcgaatt atttaatggc caaatacacc gatttgccgt
     4261 tcgtcgccat tccggtcttc ctgcaccgcc gctttcgtca cggcttcgtg ttcttgaatg
     4321 cgagcaaagg cattactagc cccagcgatt tgattggccg caaagtcggc ctgcgcaatt
     4381 ttcaagcaac ggctaatctt tggattcgtg gaattttaga gcatgaatat ggcgtgcccc
     4441 atcgcagctt gatctggctc aaacaagacg acgaagaagt tgattggacg ccgccggcgg
     4501 atttgaaaat ccagcgcatc ccggccggca aaaacatcga caagatgctg gtcgacggcg
     4561 agatcgacgc tttgatccat cctgagctga ttcagccgat cctcgacaag gatccgcgtg
     4621 ttagccgatt gtttcccgac tatcgcgatc tggaattgca ctacttcaag cgcaccggaa
     4681 ttttcccgat catgcacacc accgcaatcg cgcaaaaaat cgtcgataag tatccctggg
     4741 tgccgcttaa cctgtatcaa gccctggaag catctaagaa agccgcttac aaacgtatgg
     4801 aaaacccgcg cattgtgccg ctcgcttggt tccgttcgtt tttggaagaa caagaagaac
     4861 tgctcggccc agatccttgg gttaatggtc tcggtgatgt caaccgcaaa gcgctcgaaa
     4921 cgttgatgca gtattcccaa gaacagggtt taatcgggcg taagatgact ttagacgaac
     4981 tgtttataag ctgtgaggct cactcctagt catggaaacc gcgcttttcc gtatataatc
     5041 gccgacggtc aaatctaagc gtcgaagctg cgcgccggag tatcgaatcg cgaaaggata
     5101 cggtaatttc gcccaatcgc actgaagatg cgtatgttga aacttcgagt ctaatatctg
     5161 ctcatctacc gatggccatg aactgcgcag ctcgctcttg gccgctaaaa catcggattg
     5221 aacttacgcc gctattggcg taaattttcg ccacttaacg attgcgccgg gattgatcgg
     5281 taataaatcc ggcgaaggag gcaacatgtt tggcaaaggc gtatttcaag cggaagagaa
     5341 tctcctacgc gagatcgtcc gtagtatcga caagaaaatc gattactccg cccgcgaagg
     5401 cgaagggagc aaattcactc tgcaccttaa gttcaagaac catgaaggcg atgtcgtact
     5461 cgacctcgaa gacttgaaga acgccaagca agatcagatt cgccgccacc agattcgcca
     5521 gaaaataaaa gcacgctgcg atcacctgga cacctcccgc tatggtgccg acatcctcgg
     5581 cctgcgatcg gcgcgaatat tgaaaaccgc gcctaagctc gtaccgctgg cgcagcgcag
     5641 ctttaaccgc aacgcaccgc gacggtaatc gtaagatatc gcaattcgtt ttactttcgg
     5701 gcggttaaaa agtcgaggcg atatggcaaa agcagaatta atacgtcagg cgttagccac
     5761 ccggggccag ttgatcatgc ctggggtata cgatgcgttg agcgccaaaa ttgccgctcg
     5821 tgccggtttc gaagttacgt ttataaccgg ttatagttta tcggcaactc ttttgggcga
     5881 acctgacttc ggtttattga cgcaaaccga agtcgttggt gccgctcagc gcatttgttc
     5941 ggtaatcgat ataccggtga tcgtcgacgc cgacaccggt tatggtaatg ccatcaatgt
     6001 caccagaacc gttcaagatt tgatccgcgc cggcgccggc gggatgtttt tagaagacca
     6061 agtctggccc aaacgctgcg gtcatatgcg tggtaaacaa gttattcctt tagaggaaca
     6121 gctcaacaaa ttgcgcgcgg cagtcgaagc aaaaggtcag aatgactttt tcatagtcgc
     6181 ccgcactgac tcccgccagg ctcatggcct tgacgaagcg atcacgcggg gtatcgcttt
     6241 tagtgctgcc ggcgccgatg cggtttttat cgaagcgccg gaatccaaag aagaaatgcg
     6301 cgagatcgcc aaacatgtca aaggtcatct ggtggccaac atgttggagc gcggggtcac
     6361 gccgttgatg cgacccaatg agctaaagga acttggcttt gacttggtcg tttggccatt
     6421 ggcgccactt tattcagtgg cgaaatcgct cactgatgtc tatacaacgc tacgcaagga
     6481 aggatcgacg ctgtcgattc tcgatcgatt gatgccattt gacgagttta acgacattgt
     6541 cggcttgaat gagaaatatt tgctcgatgc caaattcaaa acttagcctg agaattagcc
     6601 tgaaccgaaa ttgagttaat tcctagagtc gcatcggcat gagaacgtaa agatagccat
     6661 cgacaccggc cttacggata acgctggggc tgagttcgtc tttcaattcg aactcgatat
     6721 ctccctcacc gcctaaaacc gctaaaacat ctaataagta acgagcatta aaaccaatgg
     6781 cgatggtttt gcctttatag tcagcctcga cttcttccac cgcttcgcca agatctgggt
     6841 tattcgctga aatcgacagt ttgccattgg caaactccat cttgacgccc ttgtaacgtt
     6901 cgctggaaag aatcgaaacc cggcgtaaag ctcgcaatag ctcttcatgg tcgagtttcg
     6961 cgatatttgg attaccgacg gggatgacct tagtatagtc ggggaaatcg ccatcgatta
     7021 ggcgcatgaa aagctcgacc ttgtccttgc tgacgagccc catgttttct ttgaaaccca
     7081 aagacacggc gccgtcttcg ccgctttcca atagcttctt tagctcggac aatccctttc
     7141 tcggtaaaat cacccctttg gtcaggccaa gtgagcccac atgcttctct tcgaaagcca
     7201 ggcgatgacc gtcggtggcg accatgcgta ccttgccgcc ctcactctgt tcgatgtaaa
     7261 ccccattaag actataacga gtctcgtccg tcgataccga aaagatcgtg cgttcgatca
     7321 tttcgcgcac agttgccgcc ggcgtcgtcg atagtccact gctatcgaat tgaggaaact
     7381 gaggaaactc cttggcatcc atgccgaata ttttaaaaac cgatttacca ctgcgtatct
     7441 cgacccagtc gttgtccaag cgcttcaact ggaccagatc gttgggcact tccctgacta
     7501 tttcataaag ctttttggcg ttgaccgtta cactaccttc ttttatgatc tcgccgtcga
     7561 cacccccgcg gacacccact tcgagatccg tggccgtgat gcgtatgccg cttctaagcg
     7621 cttcgatcat gacattagcg aggatcggca tggtattacg ccgctcgaca atgctttgag
     7681 tccaataaag cgttgcgagg agatctcccc gcttggcttt aatttccata cttcctcccg
     7741 gcgatttctt ctgcgcttat atatatataa aaataagtag taatagtagg ggctgttgga
     7801 attgtgggca aacgcattta agaagataat tcggtaagat agcttgtggt taacttgtgg
     7861 cgattatacc gctcatccac acctcatgag gaagtgtagc ggtcccggca aagttatgga
     7921 cagattgaaa gggtattgag aacaggctat ccacaactac ttgtggatat ttaaattgcg
     7981 ctcgatcatc gcaatggtcg cttgcatatt ggggtcatct ctgatgcgtc gctcgatagt
     8041 tttcgaagcg tgaataacag tagaatgatc tctgccgcca aatttgtcgc cgataccagg
     8101 gaatgaggtc tgggtatgtt tgcggcacaa gtacattgcg acttggcgtg gaaaggcgag
     8161 attttgggtg cgtctgtgtg ctttcaagtc gcttatttta agattgtaat ggtcgcagat
     8221 gcttttctgg ataccctcga cggtgatttc cctctcgtaa ctttttaagt tattgcgcag
     8281 gacatccttg gcaaagtcga tcgtaatggc agccttggtg agcgaataga aagcacccaa
     8341 gcgggtcagt gagccttcca actcacgtac gttggagctg atgttcgatg ccagaaagat
     8401 cgcgacatca cctggcagcg gtacgccctc aaattcagcc tttttttgca gaattgcgac
     8461 tctggtctcc atgtccggcg gctggatatc cgcgatcagg ccccattcga aacggtttct
     8521 caggcgatcc tctatggcgg gaatgtcttt gggaaattta tctgaggtaa tgacaatctg
     8581 cttatgggac tcgtaaagag aattgaaagt atgaaaaaac tcttcttgag tgcgctcttt
     8641 gccggcgatg aactgaatgt cgtcaataat aaggatgtcg acatttctga attttttctt
     8701 aaattcgtcc atcttatcgc ggcgcagcga actgatcagc tcgttcatga acgattccga
     8761 actaagatat acgactttta aattcggcct tttagccgcc gcttgatggc ctaaggcatt
     8821 aactaggtga gttttaccga gcccgacccc gccatagata aacagcgggt tgtaatgttc
     8881 tccaggttgg ttggcgacag ccatgcaggc cgcgtgggca aactgattgc ttgtacctat
     8941 aacaaagtta tcgaatgtgt acttccgcgc gagataacat tggcgtggct gacgttcgcg
     9001 ctctttgtcg tctttgcgct ggagctcttc ctgcggattg gattggtcgc cgtttcgctc
     9061 aacttcgata acaaccttca ggtcgtgttt cgcaaccgcg ctgacggctg attcgaggtc
     9121 ggcgagatag ttttgtccga cccagtcgcg aaaaaactta ttggctaccg ccactatcaa
     9181 ttcgttttta ttcctcgata caaaccggat tggc
//
LOCUS       DASQKS010000087         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5085778, whole genome shotgun sequence.
ACCESSION   DASQKS010000087 DASQKS010000000
VERSION     DASQKS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5085778"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VE170_02330"
     CDS             complement(<1..440)
                     /locus_tag="VE170_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYJ14294.1"
                     /translation="MAKYLITGGAGFIGSHLCEYFLNQGHQVLCMDNYSTGANENIGA
                     FLKNPRFRFIDHNVSRYIEVPEPLDFVLHFASPASPVDYLELPIPTLKVGALGTHNSL
                     GLAKEKGATYLLASTSEVYGDPLVRPQSEEYWGNVNPIGPRGVY"
     gene            complement(433..1737)
                     /locus_tag="VE170_02335"
     CDS             complement(433..1737)
                     /locus_tag="VE170_02335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYJ14295.1"
                     /translation="MKLAVVGTGYVGLVAGTCLAESGNDVICVDIDAQKIDMLKGGKV
                     PIYEPGLEEMVRRNAEDQRLSFTTDLSSAVQKSDVIFIAVGTPQGPTGHANLEYVKAT
                     AKGIAKALDSFKIIVTKSTVPVGTADQIKQWIGEETGQPFAVASNPEFMKEGAAVEDF
                     MKPDRVVLGGDHAEALATLRELYEPFVRTGNPILVMDSRSAEMSKYAANAMLATKISF
                     INEVARLCEEMNADIGEVRRAISLDRRIGPHFIFPGAGYGGSCFPKDTRAMIGMGGDR
                     QEMVLLKAVEQVNERQKRLMFDKVRRHFGDKLAGMTFAVWGLAFKPRTDDMRDAPAIT
                     IIEALLGAGARVQAFDPEAMEEARKIFGDRIQYTHRNYDALHGAAALLIVTEWNEFRR
                     PDLTRVKNLLTHPVIFDGRNVYEPAELRKLGFTYYSIGRSDG"
     gene            complement(1734..2687)
                     /locus_tag="VE170_02340"
     CDS             complement(1734..2687)
                     /locus_tag="VE170_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ14296.1"
                     /translation="MELSIVIPVYNEAENVEPLIGEIEVALLALGKSYEVVVVDDGSC
                     DGTFFKLSQMRQCLPQLKVIGLKRNFGQTAALAAGLAHAQGKVVVLMDGDGQNDPADI
                     PALLAKLDEGNDLVAGWRHNRQDPFLNRRLPSMIANRLISLTTHVKLHDYGCTLKALR
                     QDVAKSLRLYGEMHRFIPAIAYERGARIAEMKVNHRPRLRGQSKYGMTRILRVVLDLM
                     TVKFLSSYSTRPSHVFGPIGIGSGLAGFGIGLYLTVQKVFYGQDIGARPLLLLGILLI
                     FIGFQFVTMGLLGEMLARTYHESQDRPIYVVGEILEGRTGA"
     gene            complement(2692..3597)
                     /locus_tag="VE170_02345"
     CDS             complement(2692..3597)
                     /locus_tag="VE170_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYJ14297.1"
                     /translation="MSVLVVGTVAFDSIEAPAGCAERILGGSASYFALGASYFAPVRM
                     VGVIGQDFPQDHLDLFTQRGIDIAGIKREQGDTFHWRGRYHEDINQRDTIELHLNVLA
                     GFKPKLPDSYRDAEFVFLGNIDPVIQMDVVDQIRHMKLLVCDTMDYWIRESNADLKKL
                     LQRVEMLVLNDSEARLLSGYDNIVKAARTILRMGPKMVLIKRGEYGVLQFSDSAIFAT
                     PAYPLEEVFDPTGAGDSFAGGLMGQLARTGDLSQGGLRRAIVYGSVVASFTVEDFGVK
                     RLIDISLPVIEERYRRFVELTDFHS"
     gene            complement(3594..4454)
                     /gene="mtnP"
                     /locus_tag="VE170_02350"
     CDS             complement(3594..4454)
                     /gene="mtnP"
                     /locus_tag="VE170_02350"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HYJ14298.1"
                     /translation="MNPPTVGVIGGSGLYQIEGLTGVRQVAVSTPFGKPSDKLARGKL
                     GNVDIVFLPRHGKGHRWLPTEINFRANIFAMKKLGVERIISVSAVGSLRAEIAPGHLV
                     VPDQFIDRTSQRPNTFFGKGLAAHVSLADPVCRELTAVLAQSAEQAGATVHRGGIYLC
                     IEGPQFSTRAESQLYRSWGAHVIGMTNLQEAKLAREAEICFATLALATDYDCWNEQAG
                     DVEIERVLAVLHNNVALARRTIGSAVAALPLQRTCGCASALKDAIITDRRAIPKKLRQ
                     DLRPIIGKYL"
     gene            complement(4451..5539)
                     /gene="rlmN"
                     /locus_tag="VE170_02355"
     CDS             complement(4451..5539)
                     /gene="rlmN"
                     /locus_tag="VE170_02355"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYJ14299.1"
                     /translation="MKSDEKPNIKDLRESEFAEFLAAARQPGYRAKQIYEWLFREHAT
                     DFAAMTNLPAALREQLNESFTIGRLAILRRHDSADGTVKFLFGLGDGQSIESVLIPEK
                     RRLTLCISTQVGCAYGCAFCATALLGFKRNLTCGEIVEQILEANRTLSDHRRISNIVV
                     MGMGEPLANYAQTLRALEIMVDAAWGLGIAPRKITLSTVGLVPQIQKLMDQTNVTLAV
                     SLHAATQELRGRLMPVNRKYSLEELIDCCRNLPLPRRKRITFEYVLLGDVNDSVADAR
                     ALCRLLSGVPSKVNLIPFNPHPGSEFHRPSDEAVKRFQQVLFDHKLQVNVRRPRGDDI
                     AAACGQLQGQVQRELPSSVAPDAADLNP"
     gene            complement(5566..>5854)
                     /locus_tag="VE170_02360"
     CDS             complement(5566..>5854)
                     /locus_tag="VE170_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYJ14300.1"
                     /translation="EQAQAFYTVHKARPFFQGLCDYMSSGPIFVSVLEGDNAIAKNRE
                     VMGATDPAKAAAGTIRQEFGQDIEKNAVHGSDAPETAAAEIAFYFKPDELF"
BASE COUNT         1292 a         1752 c         1600 g         1210 t
ORIGIN      
        1 tcgtaaacgc cgcgcggccc gatgggattg acattgcccc agtactcttc gctctgcggc
       61 cgcaccagtg ggtcgccata aacctccgac gtcgaagcga gcagataggt tgcccctttc
      121 tctttcgcca ggccgagaga gttgtgggtg cccaaggcgc caaccttcaa agtcggtatc
      181 ggcaattcta aatagtccac cggacttgcc ggcgaggcga agtgcaggac aaagtccaag
      241 ggctccggca cttcgatgta gcggctgacg ttgtgatcga tgaagcgaaa gcggggattt
      301 ttcagaaacg cgccgatgtt ttcattggca ccggtggaat agttatccat gcacaagact
      361 tggtgccctt gattgagaaa gtactcgcac aggtgggagc cgatgaaccc ggcgccgccg
      421 gtgattaaat atttagccat cacttctccc gatggaataa taggtaaatc ccaatttgcg
      481 caattccgcc ggctcgtaga cattgcggcc atcgaaaata accggatggg tgagcaggtt
      541 tttgacccgg gttaagtccg gccggcgaaa ctcgttccac tcggtgacga tcagcaacgc
      601 cgccgcgcca tgcagcgcat cgtagttgcg atgggtgtat tgaatacggt cgccgaaaat
      661 ttttcgcgct tcttccatcg cttcgggatc gaaggcctgc acccgggcgc cggcgcccaa
      721 caacgcttcg atgatggtga tcgccggcgc gtcgcgcatg tcgtcggtgc gcggcttgaa
      781 cgccaacccc cacacggcaa acgtcatccc ggcgagctta tcgccgaaat ggcgtcggac
      841 tttgtcgaac attaatcgct tctgccgctc gttgacctgt tccaccgctt tgagcaggac
      901 catctcttgc cggtcaccgc ccatgccgat catggcgcgg gtatccttgg gaaaacagga
      961 accgccataa ccggccccgg ggaagataaa atgcggcccg atgcgccggt cgagactgat
     1021 cgcccgccgc acctcgccga tatcggcgtt catctcttcg cacagccggg cgacctcatt
     1081 gataaatgag atcttggtcg ccagcatggc attggcggcg tacttactca tctccgcgct
     1141 gcggctgtcc atgaccagga tgggattgcc ggtgcggaca aaaggctcgt aaagctctcg
     1201 cagtgtcgcc aaggcctcag catgatcgcc gcccaaaacc acccggtcgg gcttcatgaa
     1261 gtcttccacc gccgcgccct ccttcatgaa ctcggggttg gaagcgacgg caaagggttg
     1321 accggtctct tcaccgatcc actgcttgat ctgatccgcc gttcccactg ggacggtgct
     1381 tttagtaacg atgattttga atgaatcaag cgctttagcg atccctttgg cagtcgcttt
     1441 gacgtactcc aggttggcat ggccggtcgg gccctgaggc gtgcccacgg cgatgaaaat
     1501 aacatccgat ttctgcaccg cgctggacag atccgtggta aagctcaagc gttgatcttc
     1561 ggcgttgcgc cgcaccatct cttccaagcc tggctcatag atgggcacct tgccgccctt
     1621 cagcatgtca attttttgtg cgtcgatatc gacgcagatc acatcgttac cactttcagc
     1681 gaggcaagtc cccgccacta gtccgacata accagttccg acaactgcca atttcatgcg
     1741 ccagttctcc cttcaagaat ttcgccaacc acataaatcg gccggtcctg ggactcatga
     1801 taagtccgcg ccagcatttc acccaataaa cccatggtga cgaattgaaa gccaataaag
     1861 attagcaata tgccgagcaa caacaacgga cgcgcgccaa tatcctggcc atagaaaacc
     1921 ttttgcacgg tgagataaag gccgatgcca aagcccgcca ggccgctgcc tatgccgatg
     1981 ggcccaaaca catgggacgg ccgggtcgaa tagctgctca agaactttac cgtcatgaga
     2041 tccaacacca cgcgcaagat gcgggtcatc ccgtacttcg actggccgcg caggcggggc
     2101 cgatggttga ctttcatttc ggcgatgcgc gcgccgcgct cataggcgat ggctggaata
     2161 aaccggtgca tttcgccgta caggcgcaaa cttttcgcga catcttgacg gagcgctttc
     2221 aaggtacagc cgtagtcatg cagcttcacg tgggtggtca acgaaatcag ccggttggcg
     2281 atcatcgacg gcaaccgccg gttgagaaac gggtcttgtc ggttatgccg ccagcccgcc
     2341 accagatcat taccttcgtc gagcttggcc aacaacgcgg gaatatcggc cggatcattc
     2401 tgcccgtcgc catccatgag gactacaacc ttgccctggg cgtgggccaa accggccgcc
     2461 aacgccgcag tttggccgaa gttgcgcttg agaccaatga cctttagctg cggcaagcac
     2521 tggcgcatct ggctaagctt gaaaaaggtt ccgtcgcagc tgccgtcgtc gacaacgacg
     2581 acttcataac tttttcccag tgccaacaag gcaacttcga tctcgccaat cagcggctcg
     2641 acgttctctg cttcgttgta aaccggaatg acgatcgaaa gctccatcgg ctcaagaatg
     2701 aaaatcggta agctcgacaa aacgtcggta gcgttcctca attacgggca gagagatatc
     2761 gatgagccgt ttgacgccaa agtcctcgac ggtaaacgac gccaccaccg agccgtagac
     2821 gattgcccgg cgcaggccgc cctggctcag atcgccggtg cgggccagtt ggcccatcag
     2881 gccgccggca aaggaatcgc cggcaccggt gggatcgaag acttcttcca aagggtacgc
     2941 cggcgtcgcg aaaatcgccg aatcggaaaa ttgcaaaacc ccatactcgc cgcgcttgat
     3001 taaaaccatc ttggggccca tgcgcagaat cgtccgcgct gccttgacga tattatcgta
     3061 gccgctcagc agacgcgctt cggaatcgtt tagcaccaac atctcaaccc gctgcaggag
     3121 cttttttaga tctgcgttgc tctcgcggat ccagtaatcc atggtgtcgc acacgaggag
     3181 cttcatgtgg cggatctggt cgacgacatc catctgtatg actggatcga tattgccgag
     3241 aaatacaaac tccgcgtcgc gataactgtc cggcagtttc ggcttgaaac cggctagaac
     3301 attcaagtgc aattcaatgg tatcgcgctg attgatgtcc tcatgatagc ggccgcgcca
     3361 atgaaatgta tcgccctgct cgcgcttgat gcccgcgata tcgatgccgc gctgggtaaa
     3421 caggtccaag tgatcctggg ggaaatcctg gccgatgacg ccaaccatgc gtaccggcgc
     3481 gaaataactc gcgccgaggg cgaaatagga cgccgatccg ccgagaattc gctcggcgca
     3541 acccgccgga gcctcaatcg aatcgaacgc aaccgtgccg acaaccaata cactcatagg
     3601 tattttccta ttatgggccg caaatcttgc cgtagttttt taggaatcgc ccgtcgatcg
     3661 gtaataatag catcctttag cgcgctcgca cagccgcaag ttcgctgcag cggcaaagca
     3721 gccaccgccg agccgatggt gcgccgcgcg agtgcaacat tgttgtgcaa gaccgccaag
     3781 accctttcga tttcaacatc gccggcctgc tcgttccaac aatcgtagtc ggtagccaag
     3841 gccaatgtgg caaaacagat ctccgcttcg cgggccagtt tggcttcctg caaattggtc
     3901 ataccgatca cgtgcgcacc ccaactacgg tacagctggg actctgccct ggtggagaat
     3961 tgcggcccct cgatacacag gtaaattccg ccgcgatgca ccgtggcgcc cgcctgctcg
     4021 gcggattggg cgagaaccgc ggtgagctct cggcaaaccg gatcggccag gctgacatgg
     4081 gcggccagtc ccttgccaaa aaatgtattc ggccgctggc tggtgcgatc gatgaattgg
     4141 tcgggcacga ctaaatggcc gggagcgatt tccgcgcgca gactacccac cgcgctgacc
     4201 gagataattc gctcgacgcc caacttcttc atggcgaaaa tattcgcgcg aaaattaatt
     4261 tccgtgggca gccagcgatg gcctttgccg tggcgcggca aaaacacaat atcgacattg
     4321 ccgagtttgc cgcgcgcgag cttgtcagag ggtttgccaa acggcgtcga caccgccact
     4381 tgccttaccc ctgtgagacc ttcgatctgg tacagcccac tgccgccgat cacgccgacc
     4441 gttggtggat tcatgggttt aagtctgccg cgtccggcgc cacggagctg ggcaattccc
     4501 gctggacctg cccctggagt tggccgcacg ccgccgcgat atcgtcgccg cgcggacggc
     4561 gcacgttgac ttgcaactta tgatcgaaaa gaacttgctg aaaacgcttg acagcctcat
     4621 cgctgggccg atgaaattca ctgccgggat ggggattgaa cggaatcaag ttgaccttgc
     4681 tcggcacacc gcttaagagc cggcatagcg cgcgggcatc cgccaccgaa tcgttcacgt
     4741 caccgagcaa aacatactcg aaggtgatcc gtttgcgtcg cggcagcggc aaattacggc
     4801 aacagtcgat caactcctca agcgaatact tgcggttgac cggcatcaag cggccgcgca
     4861 actcctgggt ggcggcgtgc aacgacaccg ccaatgtgac attggtttgg tccattaact
     4921 tttggatttg cggcaccaac ccgacagtcg acaaggtgat ttttcgcggc gcgattccca
     4981 atccccaggc ggcatcgacc atgatttcga gggcacgcaa cgtttgcgca tagttggcca
     5041 acggttcgcc cattcccatg accacgatat tggatatccg ccggtggtca gacagcgtgc
     5101 ggttggcttc aagaatctgc tcgactatct cgccgcaagt gagatttcgc ttaaaaccca
     5161 aaagcgcggt ggcacaaaaa gcacagccgt aagcacaacc cacctgggtg gaaatacata
     5221 gggttaaacg gcgcttttct ggaattaaga cgctctcgat actctggccg tcgccgagac
     5281 caaagagaaa ttttaccgtc ccgtcagcgg aatcgtgccg gcgcaggatc gccaaccggc
     5341 cgatggtgaa agactcgttc aattgctcgc gcaatgccgc cggcaggttg gtcatggcgg
     5401 caaaatcggt ggcgtgctca cgaaacaacc actcgtagat ttgtttggcg cgatagccgg
     5461 gctgcctggc cgcggcgaga aattccgcga actcgctttc gcgcaaatcc ttgatattgg
     5521 gcttctcgtc tgacttcacg ttaagccacc caatatggcg caagcctaaa acaattcgtc
     5581 gggtttgaag tagaaagcga tctctgcggc ggcggtctcc ggtgcatctg agccatgcac
     5641 cgcattcttc tcgatgtcct ggccaaactc ctggcgaatc gtgccagcgg cggctttcgc
     5701 cgggtccgtg gcgcccatca cttcgcgatt cttggcgatc gcgttatcgc cttcgagcac
     5761 cgacacgaag atcggtccgg acgacatgta gtcacacaag ccctgaaaaa aaggccgcgc
     5821 tttatgcacg gtataaaacg cctgcgcttg ctcc
//
LOCUS       DASQKS010000088         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1727306, whole genome shotgun sequence.
ACCESSION   DASQKS010000088 DASQKS010000000
VERSION     DASQKS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727306"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VE170_02365"
     CDS             complement(<1..647)
                     /locus_tag="VE170_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HYJ14301.1"
                     /translation="MLIICLLIFAALPLYTVLSGQAPQGRDFRSADRSSAGIAPSAES
                     TPEAVVQVYFARALNWRGIFGVHTWVATKPANATEYTVHQVIGWRIYRDLPAVSSTTG
                     IPDARWFGNAPTLIADLRGDAAAKAIPKVIEAIASYPYANQYAVWPGPNSNTFVAFIG
                     RAVPELRMDLPSTAIGKDYPINGSMIDRTPSGTGYQLSMLGVLGVTMAREEGFEL"
     gene            852..1334
                     /gene="ispF"
                     /locus_tag="VE170_02370"
     CDS             852..1334
                     /gene="ispF"
                     /locus_tag="VE170_02370"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HYJ14302.1"
                     /translation="MAKQFRIGHGFDVHRFRRGRKLILGGVEIPSPVGLLGHSDADVV
                     LHALINALLGAMGEGDIGSHFPDTDRRYKGIASAKLLAQVLAIMRRKRFNIVNVDVTL
                     VAQRPKLSLHYPAMRASVAGLLKLAVSDVSIKAATTEKLGWLGKGQGMAGTAVILLCR
                     "
     gene            1401..1751
                     /locus_tag="VE170_02375"
     CDS             1401..1751
                     /locus_tag="VE170_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14303.1"
                     /translation="MTSDTPNLAPEAREQEIERLAQELGRLIQGADPERRHDLKELAI
                     ALMREEFIHKAEPAPGAGATPAPFNPLGTGVLLFFLGGGLSFVFGPVGLLLMLGGITF
                     VVWGALISWLKKAK"
     gene            1754..2272
                     /locus_tag="VE170_02380"
     CDS             1754..2272
                     /locus_tag="VE170_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HYJ14304.1"
                     /translation="MAEAEKKRGGIWSRRDFFTRLGWGGFGVFGGLSLLGFLRSAFPR
                     VLFQPPATFKAGFPSDYAVGEVSEKYKQDQRVWIVREETGVYAIFAKCTHLGCTPRWL
                     AAESKFRCPCHGSGFYKSGLNFEGPAPRPLDRFLLTVGDDGQLVVDKNKIFKGTAGAD
                     PDEQYPESILKA"
     gene            2284..3054
                     /locus_tag="VE170_02385"
     CDS             2284..3054
                     /locus_tag="VE170_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HYJ14305.1"
                     /translation="MSPLEKIKTDIVESQVWKAIFRHGYDDTPRNRVMMVTSNVFLHL
                     HPAKVRRHAVRMRFTWCMGGLTFLMFLITAITGIYLMFYYRPVAEYAYADMKYLEFDM
                     PFGMLMRNMHRWAAHAMIVFVWLHMFRVFLTGSYKPPREFNWIVGVLLLVLTLLLSFT
                     GYLLPWDQLAIWAVTVGSNMARATPLLGYEGPFAEFVGANPIYDARAFLFGGGEIGPH
                     TLLRFYILHCIFIPLVASILMAVHFWRIRRDGFSGPSL"
     gene            3079..>3380
                     /locus_tag="VE170_02390"
     CDS             3079..>3380
                     /locus_tag="VE170_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HYJ14306.1"
                     /translation="MAEAAQERKLPVDDKIHTWPHLVRMEFLVMLFVMIGLTIWSLTI
                     DAPLEEAANPTKTPNPSKAPWYFLGLQEMLVYFDPWHAGVVLPTLIIVGLMVIPFI"
BASE COUNT          675 a          902 c         1014 g          789 t
ORIGIN      
        1 ttcagttcaa atccttcttc ccgagccatg gtcacaccga gaacgccgag catggagagc
       61 tggtagccag tgccgctcgg ggtgcggtcg atcatggagc cgttgatcgg atagtccttg
      121 ccgatggcgg tggagggcag atccatgcgc agttccggga ccgcgcggcc gatgaaagcg
      181 acgaaggtat tgctattggg cccgggccag actgcgtatt ggttggcata aggatagctg
      241 gcgattgctt cgatgacctt ggggatcgct ttggcggcgg cgtctccgcg cagatcggcg
      301 atcaacgtcg gagcgttacc gaaccaacgg gcgtcgggga tcccggttgt cgaggagact
      361 gcgggtagat cgcgataaat gcgccagcca atgacctgat gcaccgtata ctctgtggcg
      421 tttgccggtt tggtggcgac ccaggtgtgg acgccgaaaa tgccgcgcca attgagcgcc
      481 cgggcgaaat aaacctgcac caccgcttcc ggtgtcgatt cggcgctagg cgcaatgcca
      541 gcgctggagc ggtccgcgct gcgaaagtca cgcccctgcg gcgcttgacc ggagagcacg
      601 gtataaagcg gcagggcggc aaaaataagc aagcagatga ttagcattcg catgggtctt
      661 gaacttggcg cgaagtatag cggatagcgg tattgaacac cataactcct ataacgttgt
      721 gtgcatcgct gcgatgggtg attcccttgg gtcggaataa gccaatttcg gacggaattt
      781 ttacttcgaa acggcaatcc ggttgaatgt cgcggtggtt cgggggatga tgttttctcg
      841 acagaaaagc gatggcaaaa cagtttcgta tcggtcatgg tttcgacgtg caccgctttc
      901 gtcgcggccg gaaattgatc cttggtggcg tcgagattcc gtcgccggtc ggtctgctcg
      961 gccattccga cgccgacgtg gttttacacg cgctgatcaa cgcgttgctg ggcgccatgg
     1021 gcgaagggga tatcggcagc catttccccg ataccgatcg tcgttacaag gggattgcca
     1081 gcgccaagtt acttgcccaa gtgctggcga tcatgcggcg caaacgtttt aacattgtca
     1141 atgtcgacgt gactttggtg gcgcagcggc ccaagctgtc gctccactat ccggcgatgc
     1201 gcgcgagcgt tgccggtctg ctcaaacttg ccgtaagcga cgtcagcatc aaggccgcca
     1261 ccacggagaa gctcggctgg ctcggcaaag ggcagggcat ggcggggact gcagtgattt
     1321 tgctctgtcg ttaacggtag agaagggcca aattgttgac aaagaatatt ttctataata
     1381 atcaaccatt ctaatctcat gtgacgtccg atacacccaa tctagcgccg gaagcgcgcg
     1441 aacaagagat cgaaaggttg gctcaagagc ttggccgtct gattcagggc gccgaccctg
     1501 agcggcgcca tgatctcaag gagttggcca tcgcgctgat gcgcgaggag ttcatccaca
     1561 aggctgaacc ggcgccgggt gcgggagcga caccggcgcc tttcaatcca ctgggaaccg
     1621 gagtcctgtt gttcttttta ggcggcggct tgtcgttcgt attcggcccg gtagggctgc
     1681 tgctgatgtt aggcggcata acttttgtag tttggggagc cttgatcagc tggcttaaga
     1741 aagcgaaata gttatggcag aagcggaaaa gaaaagaggc ggcatctggt cacggcgaga
     1801 tttcttcacg cgtttgggct ggggcggttt tggcgtattc ggcggcttga gtcttctcgg
     1861 gtttctccgg tcggcgttcc cccgggtgct gtttcaaccg ccggcaactt ttaaagccgg
     1921 gtttccttcg gactacgcgg ttggcgaagt cagcgagaag tataaacaag accagcgggt
     1981 ttggatcgtc cgcgaagaga ccggcgttta tgcgattttt gccaagtgca cgcacctggg
     2041 ttgcacgccg cgctggctcg ccgcggagag caaattcaga tgtccctgtc acggcagcgg
     2101 cttttataag tcgggcttga atttcgaagg acccgcgcca cgcccgctcg atcgttttct
     2161 gctaactgtc ggcgacgatg gccagctggt ggtcgataag aacaagattt tcaaaggcac
     2221 ggcgggggcc gatccagacg agcagtatcc cgaaagcatc ctcaaagcct agcggaaaaa
     2281 atcatgagcc cattagaaaa gatcaaaacc gatatcgtcg aaagtcaagt ttggaaggcg
     2341 atcttccgtc atggttatga cgatacgccg cgtaaccggg tgatgatggt gacttccaac
     2401 gtgttccttc atctccatcc cgccaaggtt cggcgccatg ccgtgcgaat gcgctttacc
     2461 tggtgcatgg gcggactcac atttctcatg tttctcatca ccgcgattac cggcatttat
     2521 ctgatgttct actatcggcc cgtggcggag tacgcctacg ccgacatgaa gtatctcgag
     2581 ttcgatatgc ccttcggcat gctcatgcgc aacatgcacc ggtgggcggc ccacgccatg
     2641 atcgtatttg tctggctgca tatgttccgc gttttcctca ccggctccta caaaccgccg
     2701 cgcgagttca attggatcgt cggcgtgctg ctgctcgtgc tcacgctgct gttaagtttc
     2761 accggctact tgctgccctg ggatcaactc gcgatctggg cggttaccgt cggttccaac
     2821 atggcccggg cgacgccgct gttgggttat gaaggcccct tcgccgagtt tgtcggcgct
     2881 aatccgatct atgatgctcg tgcgtttcta ttcggcggcg gcgagatcgg cccgcatacg
     2941 cttttacgct tctatattct ccattgcatt tttatcccgc tggtcgccag catcctgatg
     3001 gcggtgcatt tctggcgcat acgccgcgac ggtttttccg gtccatcttt atagtcgctg
     3061 cgaaaacgag gcttagctat ggcagaggcc gctcaagaac gaaaacttcc ggtcgacgat
     3121 aagatccaca cctggcccca tctggtgcgc atggagtttc tggtgatgct attcgtgatg
     3181 atcggtctga cgatttggtc gctgaccatc gatgcgccgc tcgaagaagc ggctaatccg
     3241 accaaaacgc ccaacccgtc gaaggcgcct tggtattttt tgggtttgca agagatgctg
     3301 gtctatttcg acccctggca tgccggcgtg gtgctgccga ccttaatcat agtcggtcta
     3361 atggtgatcc cattcatcga
//
LOCUS       DASQKS010000089         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5938970, whole genome shotgun sequence.
ACCESSION   DASQKS010000089 DASQKS010000000
VERSION     DASQKS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5938970"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VE170_02395"
     CDS             complement(<1..550)
                     /locus_tag="VE170_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14307.1"
                     /translation="MNIDIHAHFVAADSLRLASEIGKRHGMKLEKNERGREIVSRAGK
                     PYLTQPKAEFSDLDLRLSIMDSQGVDMQAISPASTYFFYWMDAEESLAYAQWLNERLA
                     EAVATHPTRLVALGSVPMHDSAKAVVELDRAVTKLGLRGVEIASNINGRYFDDAAFNP
                     FWETAQALDALIFVHPNQVVGAD"
     gene            739..936
                     /locus_tag="VE170_02400"
     CDS             739..936
                     /locus_tag="VE170_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14308.1"
                     /translation="MREGDRILINKGIAKKVMIIARTEIPHSMERIFVTKSGGKYTIG
                     LKVWRSDYAWSDKNQMWVPKH"
     gene            1018..2379
                     /gene="holA"
                     /locus_tag="VE170_02405"
     CDS             1018..2379
                     /gene="holA"
                     /locus_tag="VE170_02405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HYJ14309.1"
                     /translation="MKSDLPTILADLRSAKGPQLLLLFGDDLQVQETCKQVVDALVPQ
                     DQRGFNFERFDGRTVSWDQVEGSLMTPPFFPGKKVLWVENAPYFFSSAQKGEFGEKIL
                     DLWREGRRDDAVKLLADLLIVEAWTQETWDRLDVAASNQLIALLDVDGSAAREDVGAL
                     LAYAKSRDLDLSKRKGADAHRLGELLDGKMPEWAFLLLSAMQVDRRTRLFKRFEELGA
                     ALFLGLTRDRYGKVDHDSLISFISQRLRQAGKNIAPQARDMIVARAGDDLRTLQQELD
                     KLLLYTAERPVIEPHDVEAMVADHGEGWIFDLTRAIGDRDALGALGQLSRLMGQGDHP
                     LKILGTIAGEVRRLIGARQLLDGDLRPHWKRGISYQQFQQIAAKQGVAALTRSPYADY
                     MCFQRAERFAMPELVGHMESLFDADLRLKSSGGQPRLVMEKLILGMCLAARRNQQSVV
                     PVR"
     gene            2440..2916
                     /locus_tag="VE170_02410"
     CDS             2440..2916
                     /locus_tag="VE170_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HYJ14310.1"
                     /translation="MAEMVVQACFSPQGKCAAHILREIDQAKKELLVAVYAFTNDELA
                     GALVQAKKRGVAVQIVIDRDFDTRMENSKGKYFDAHKIPLRRLSGTKAKSSERDAGLM
                     HQKFAVIDRKTVFTGSYNWTHSAENANDENLLLFRDAGPLAEDYRQAFLRLWERKP"
     gene            2916..3455
                     /locus_tag="VE170_02415"
     CDS             2916..3455
                     /locus_tag="VE170_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14311.1"
                     /translation="MSRTAGTGLLIVLAFAAGLFTHGLYLRWNPPAGDTPPASGSLAN
                     LPGTATAVPGQHRVTYMPLGEPVLQAEQAAEIPSLGAREVDKIRALTGKLARVRGRVF
                     RVGHSAKSNTYFLNFGPSREALTAVIFASALELFEKNQMAPKNFENKEVEITGQIKDH
                     PQYGLEIILDNPKQIKILS"
     gene            3490..3669
                     /locus_tag="VE170_02420"
     CDS             3490..3669
                     /locus_tag="VE170_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14312.1"
                     /translation="MSEAAKKCMVCAKPSDALKGGICQPCQERIRREALGEQAGVADR
                     AEREISRQGIAPTKK"
BASE COUNT          899 a         1106 c         1183 g          868 t
ORIGIN      
        1 gatcggcgcc gaccacctga ttggggtgga cgaaaattag ggcgtccaga gcctgagccg
       61 tttcccaaaa tggattaaat gccgcatcgt cgaagtagcg accgttgata tttgacgcga
      121 tctcgacgcc gcgcaagccg agttttgtca ccgcccgatc tagttcgacc accgccttgg
      181 cgctgtcgtg catcggcact gacccgagag ccaccaatcg ggtagggtga gtagccaccg
      241 cttcagccaa gcgctcgttg agccactggg catatgccaa cgattcttcg gcgtccatcc
      301 aataaaaaaa ataggtgctg gccggagata tcgcctgcat gtcgacacct tggctatcca
      361 tgatcgatag acgcaggtcg aggtcggaga actcggcttt aggttgggtc aaatagggct
      421 tgccagcgcg gctgacgatc tcccggccac gttcgttctt ctccagcttc atgccgtgac
      481 gctttccgat ctcgctggcg agtcgaaggc tgtcggcggc gacgaaatgt gcgtggatgt
      541 cgatattcat tgggtaccgc gtgactaact ccccggacag ctatcggagg gtcggctgat
      601 tgtcaagttg acaatccgtc gctagcgctc catcggcaaa tggaattggc ttggtgtaac
      661 gaaatcattg cctgtggaaa ttaccggtga tagctttggc ccatggccag caggcgcaga
      721 agagaaaacc agataaccat gcgcgagggc gatcgcatcc tgatcaacaa gggcatcgca
      781 aaaaaggtga tgatcatcgc gcgcaccgag attccccatt cgatggaacg aatcttcgtc
      841 accaaatcag gtggcaaata taccatcggg ttgaaggtct ggcgctcgga ttacgcctgg
      901 agtgataaaa accagatgtg ggtcccgaag cattaagcgt cgtgctcgcg aattgagtgc
      961 tgggcagtgt gtccagttga gccccatccc gttgacactc gaattacccg tacttccgtg
     1021 aaaagcgatt tacccactat cctcgccgat cttaggagcg ccaaagggcc acaactgttg
     1081 ttgctgttcg gcgacgatct gcaagtacaa gagacgtgca agcaagtggt cgatgcgttg
     1141 gtgccgcaag accaaagagg gtttaacttc gaacgcttcg atggccggac ggtgagctgg
     1201 gatcaagtgg aaggctcgct gatgacgccg ccgtttttcc ccggcaagaa agtcctgtgg
     1261 gtagaaaacg cgccgtactt tttctcgtct gcgcaaaaag gcgagtttgg tgaaaagatc
     1321 ctcgatcttt ggcgcgaggg ccgacgcgat gatgccgtca aactgctggc ggatctctta
     1381 atagtcgaag cctggaccca ggaaacctgg gatcggctgg acgtagcggc gtcaaatcag
     1441 ctcatagcgt tgctcgacgt cgacggcagc gcggcgcggg aagacgtcgg cgcgctgctg
     1501 gcttatgcca agagccgtga tctcgatctc agcaaacgca aaggggccga cgcccatcgc
     1561 ttgggcgaat tgttggatgg caaaatgccg gaatgggcat ttttattgct ctccgcgatg
     1621 caagtcgacc ggcgcacaag attgttcaaa cgtttcgaag aactgggcgc ggcactgttt
     1681 ctcggcttga cacgcgaccg gtacggtaaa gtcgatcacg acagtctaat cagcttcatc
     1741 tcccaacggc tgcgccaggc gggtaagaat atcgcgcccc aggcgcgcga catgatcgtc
     1801 gcccgcgccg gtgacgatct tcgtactttg caacaggagc tcgacaagct gctgctttat
     1861 acggccgaac ggccagtcat tgagcctcat gatgtcgagg cgatggtcgc tgatcatggt
     1921 gaaggctgga ttttcgatct cacccgcgcc atcggtgatc gcgacgccct gggcgcgctt
     1981 gggcaactgt cccggttgat gggccaaggc gaccatccat taaaaatctt gggtaccatc
     2041 gccggcgaag tgcgccgtct gatcggcgcc cggcagcttt tggacggcga cctgcggccc
     2101 cattggaagc gcggtatcag ctaccagcag tttcagcaga ttgccgccaa gcagggagtc
     2161 gcggcgctga cccgcagtcc ttacgccgac tacatgtgct ttcaacgtgc cgaacggttc
     2221 gccatgccgg agcttgttgg ccatatggaa tcgttgttcg acgccgatct gcggctcaaa
     2281 tcgagcggcg gccaaccgcg cttggtgatg gaaaagctga ttctcggcat gtgcctggcc
     2341 gcccgccgca atcagcaaag tgtcgttccg gttcggtaac ttatgcttcg cctgcttacc
     2401 gcacagcttc tttggctcac cctgattccc tcagtctgca tggctgaaat ggtcgtacag
     2461 gcttgtttca gcccccaggg caaatgcgcg gcgcatatcc tgcgcgagat cgaccaggcc
     2521 aaaaaagagt tgctagtggc ggtttacgcg tttaccaacg atgagctggc cggcgcccta
     2581 gtgcaggcaa aaaaacgcgg cgtcgcggtg cagattgtga tcgaccgcga cttcgacacg
     2641 cgcatggaaa attccaaggg gaagtatttc gatgcgcata aaataccgct gcgccggctg
     2701 tccggcacga aggcgaaaag ctcggagcgc gacgccggct tgatgcacca aaagttcgcc
     2761 gtcatcgacc gtaaaaccgt tttcaccggg tcttacaact ggacccattc ggcggaaaac
     2821 gccaacgatg aaaatctgtt gctttttcgt gacgctggac ccctcgccga ggactatcgc
     2881 caagctttct tgcgcctgtg ggagaggaag ccgtaatgag tcgcacggcg ggaaccggtt
     2941 tgctcattgt gctggctttc gccgccggac tgtttactca tggcttgtac ctacgctgga
     3001 atccgccggc cggcgacaca ccgccggcaa gcggttcact ggcaaatttg cctggcacag
     3061 cgaccgcggt gccgggtcaa caccgtgtga cctacatgcc gctgggcgag cccgtactgc
     3121 aagcggagca ggcggccgaa attccatcat taggggccag ggaggtggat aaaatccgcg
     3181 cgctcactgg caagctggca cgggtgcgcg gccgggtatt tcgtgtcggc cattcagcca
     3241 agagcaatac ttattttctt aacttcggtc catcgcggga ggcgctaacc gcggtgattt
     3301 ttgcttcggc gctggagctg ttcgaaaaga atcagatggc ccctaagaat tttgagaata
     3361 aagaagttga aatcaccggt cagattaaag accacccgca gtatggcttg gagataattc
     3421 tcgacaatcc gaagcaaatc aagatcctga gttagtaccc atgttcgcca atcaaggaaa
     3481 ggtgccgtca tgagtgaagc cgcgaaaaaa tgtatggtct gcgcaaaacc ctcagacgca
     3541 ttaaagggcg gcatttgcca gccgtgccaa gagcgaattc gccgcgaggc tctgggcgag
     3601 caggccgggg ttgccgaccg cgccgaacgg gaaatctcgc gccagggaat cgcgccgact
     3661 aaaaagtaaa atgaccgagc tgcttgccaa cagtacggaa ctggggattc ggctggtaaa
     3721 aatttatcgc gaggctgggc gggcctgctg ccgtgaagct aattgcccgt gctgttaccg
     3781 cagcttgaat gcggtgcgcc agtaaagcgc cgtttccacc tggggaattt tcgcgcaatt
     3841 gcggcacctc ccgtcacgaa cggatagcag ccagtgaaaa aactggtttt taattgtcct
     3901 cgttaccaag gacatgttat aaaacaatat tatcagattg gtagccgaga tccttcttgg
     3961 ctttgtatct acaccgattg agagggtaaa ggttctgcgc tgccgtcaac tattccccgc
     4021 cgcgttcagc ttggctattt tggttgtttt cgcttc
//
LOCUS       DASQKS010000090         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2988507, whole genome shotgun sequence.
ACCESSION   DASQKS010000090 DASQKS010000000
VERSION     DASQKS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2988507"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..1499)
                     /locus_tag="VE170_02425"
     CDS             complement(420..1499)
                     /locus_tag="VE170_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14313.1"
                     /translation="MKHWTCVSRLAQVFPFTAAAIALICSAVAPVRAQAPFFQGKTVT
                     IIQGRDPGGSGDLRTKALVPFLQKYLPGNPTVIMEYMPGAGGRMAANHLYRTAKADGL
                     TIGNPSIGMISSAVLGEAGVQYDIDKFSYLGSPYSTYHAVFLSRQQAGFSSVEKLQAA
                     SGVRIGAQSVGFVTYNEGRLFAYLLGLKEPRFIAAYSGAELDPALIRGEIDARATGAD
                     SITQRNKEWLEKGSPVDFHAILEVPKGNKHPQFAQVREIESFAKSDLDRKLANMARAF
                     RIAGTPFVLPPGTPRDRVEIIQAAFRKTFQDPDFSREYKKMSGDEATPLLPENHEKAI
                     KEIPRDREVIELFKKLVGAGPLPTR"
     gene            complement(1581..1973)
                     /locus_tag="VE170_02430"
     CDS             complement(1581..1973)
                     /locus_tag="VE170_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14314.1"
                     /translation="MLKTQGMLHFSLAVTDLDRSRKFYEGVLNLKVVEQSPRMVFLQT
                     GDDYLILAKGDEPLKYDTNKSTPVHHAFKVKPDEFQSSLEHLRQHGVEVFNIEDRNTG
                     VFWGPQAYFLDPDGNKLEIYAGPGAKKS"
     gene            complement(2024..3064)
                     /locus_tag="VE170_02435"
     CDS             complement(2024..3064)
                     /locus_tag="VE170_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14315.1"
                     /translation="MLLPFREHLDFGPTPYDLLLEKEGIPIVRGNVVADVTTLELGNW
                     ERMGVLGCYLNLSNQQRTDAYVCEIPPSRQTLPQRHLFEEIVYVAKGRGATTVWQDEK
                     SKVTFEWGPGAIFAIPLNATYIHFNGSGSEPIRLFVGTTCPAMINIFHNEDFIFRNPF
                     NFRDRFHEGDEFLRYNKHVTDRYWETNLVPDVNHFPLDDFPMKGKNVKHMRYTLADTT
                     YGCHVSEFPPGCRSTFHRHGPGAVIIITQGEGYVVLWRDGEERQRHDFKVGTVYSPND
                     LMWHGHFNTGTGSMRHFAMRGDSPKYSHDRFRNPAWTMIPMADEDPAIQREYVEILKR
                     NGVAAAVQVVED"
     gene            3178..3576
                     /locus_tag="VE170_02440"
     CDS             3178..3576
                     /locus_tag="VE170_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HYJ14316.1"
                     /translation="MKDSLSPGLTRSATYQTTIDMRARQLKADVFSTPSMISLMERTC
                     TELTEPFLDENEQTVGIHIDVRHMASTRIGQSVTMNAAIIEVKGRKIRYSVDAFNDAG
                     VKIGAGTQWRAVIDTQRFAGGIEPEVDHGH"
     gene            3566..4648
                     /locus_tag="VE170_02445"
     CDS             3566..4648
                     /locus_tag="VE170_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14317.1"
                     /translation="MGIRISAIRLVVALVLWTIMPARLSAQSEEKLLDEINQLPEAER
                     QTRLVAGAKKEGTVNWYVAMNRAYANDLVNGIQAEYPFLKVNTLTGSGGSLLNRVLTE
                     SRARSHQFDVFNTRSMTVNTLKKAGAIMRYRSPYRSALRDGFADKEGFLNGIFATPLV
                     FLFNTKMIPRKEAPSTIEDLLRPNWTGKMAIDDESYDWLAALIDHYGEAKGLELAARL
                     GQQKLQVRRGPSLITQLVAAGEFSLQIDSHHQEVINKKKAGAPIDYIFPQPFVPAKSL
                     IPIYMSARPPHPHAAALLADFLMSRKGQAIMHGHGRWVGHKSIATKGPDDVGERKVII
                     PSAEKWGDRYNELIGWYNKLLLRQGA"
BASE COUNT         1125 a         1436 c         1361 g         1049 t
ORIGIN      
        1 tcaccggcac agtcaagaac ggcaatgtca gcacgccgat caacggcaag ctgaacggtg
       61 aagaaatcag tttcagcggc ggcggcgtcc aatacacggg acacgttcaa ggcaacagca
      121 tggaagtcaa tggaggcgga gccaagctaa ccgcgacccg cgcaggcaag taagccagaa
      181 aacgctttaa tcaaaaacgc gcactcccaa agagtgcgcg ttttttttaa gatagactga
      241 ggtggtacgc cgatctccac ttattcgtca tgccggtgaa aaccgacatt ccagcttggc
      301 cacagcttcg gtagaggccg gaacgctgtg gactagctag gtggaactgc gggacggaag
      361 gatcttggtc aagccagccc cacctagtca acagcttccg aagtttcccg tagtcagtgt
      421 caccgcgtcg gcagcggtcc ggcgccgacc agtttcttga acagctcgat cacctcacgg
      481 tcgcgcggaa tttctttgat cgccttttca tggttttccg gcagcagcgg ggttgcctca
      541 tcaccgctca tttttttata ttcgcgcgag aaatccggat cctgaaaggt cttgcggaag
      601 gcggcctgga tgatttcgac acgatccctc ggtgttcccg gcggcagcac gaacggcgtg
      661 ccggcgattc taaaagcacg cgccatattg gcgagcttgc gatctaaatc cgacttggca
      721 aagctctcga tctcccgcac ctgggcaaat tgcggatgtt tgttgccttt gggaacttcg
      781 aggatagcat ggaagtccac cggcgaacct ttttcgagcc actccttgtt acgttgagta
      841 atcgagtcgg cgccggtggc gcgcgcgtcg atctcgccgc gaatcagcgc tggatcgagc
      901 tcggcgccgc tataagcagc aatgaacctt ggttccttta gacccagtaa gtaagcgaac
      961 aggcggcctt cgttgtaagt gacgaaacca acggactgag cgccgatgcg cacccccgat
     1021 gccgcttgca gcttctcgac gctgctaaaa cccgcctgtt gtctgctgag aaagaccgcg
     1081 tgataagtgc tgtagggtga acccagatag gaaaatttat cgatatcgta ctgcactccg
     1141 gcctcgccga gaacggcgga agaaatcatg ccgatactcg gattgccgat ggtgagcccg
     1201 tcagccttcg cggtccggta aagatgattg gcggccattc gcccacccgc gccgggcatg
     1261 tattccatga tgacggtggg attccccgga agatattttt gcagaaacgg cactaaggcc
     1321 ttggtgcgca aatctcccga cccgccggga tcgcgcccct gaatgatcgt aacggtcttt
     1381 ccttgaaaga acggcgcttg cgcacggacc ggtgccactg ccgagcatat taaggcgatt
     1441 gccgcagcag tgaaggggaa aacctgcgca agtcgcgaca cgcaagtcca gtgcttcatg
     1501 gctcctccag gtttaacttt ttacttttga aacgttaccc agtgaaaacc ctcgccgatc
     1561 ctgtcgaaat gtttgtcgct ttaactcttc ttcgcccccg gcccggcgta aatttccaac
     1621 ttattgccgt cgggatcgag gaagtaggcc tgcggtcccc aaaatacgcc ggtgttgcgg
     1681 tcttctatgt tgaatacttc gacaccgtgc tggcgcaaat gttcgagaga cgactggaac
     1741 tcatccggct tgaccttgaa ggcatgatgc accggcgtgc tcttgttggt gtcgtatttg
     1801 agcggttcat cgcctttggc gaggatcaaa tagtcatcgc cggtctgcag aaacaccatg
     1861 cgcggcgact gctcgaccac tttcaagttg agcacgcctt cgtagaattt gcggctgcga
     1921 tccagatcgg tgaccgccag gctaaagtgc aacatgcctt gagtctttaa catttgccgt
     1981 cctccattgt ttgatggcta tcgcccatta tcggtcactc tttctagtct tccaccacct
     2041 gcaccgccgc ggcaacgccg ttgcgcttga gaatctcgac atactcgcgc tggatcgccg
     2101 gatcttcgtc ggccatcggt atcatcgtcc aggcgggatt acgaaagcgg tcatggctgt
     2161 atttggggct atcgccgcgc atggcgaagt ggcgcatgct gcctgtgccg gtattgaagt
     2221 ggccatgcca catgagatcg ttcggcgagt agaccgtgcc gactttaaaa tcatggcgct
     2281 ggcgctcctc gccgtccctc cacagcacca cgtagccctc gccctgggtg atgatgatca
     2341 ccgcccccgg cccatggcga tggaacgtgc tgcggcaccc cggcggaaac tccgagacgt
     2401 gacagccata agtcgtatca gccagagtgt agcgcatatg cttgacgttc ttgcccttca
     2461 tcgggaaatc gtccaaggga aagtgattga cgtcggggac taaattggtc tcccagtaac
     2521 gatcggtgac atgtttgttg taacgcagaa actcatcgcc ttcgtgaaag cggtcgcgga
     2581 agttgaacgg attgcgaaaa ataaaatctt cattgtgaaa aatattgatc atggccgggc
     2641 aggtcgtgcc cacgaacagc cggatcggct cgctgccgga accgttgaaa tggatgtagg
     2701 tggcattcaa tgggatggcg aagatcgccc ccggccccca ctcgaaggtg actttcgact
     2761 tctcgtcctg ccagaccgtc gtcgcgccgc gccctttggc gacataaacg atttcctcga
     2821 acagatggcg ctgcggcagg gtttgacggc tcggcgggat ctcgcaaacg tatgcatcgg
     2881 tgcgctgctg gttggagaga ttgagatagc agccgagcac ccccatgcgc tcccaattgc
     2941 ccagctccag agtggtcacg tcggctacga cattgccccg gacgatgggt atcccctctt
     3001 tttccagtaa aagatcatac ggcgtcggcc caaagtctaa atgttcgcga aacggtaaaa
     3061 gcattggctc actcctaaac agtttcatct tccatagcac gaacgcgcac tacgatcacg
     3121 tgctaatcca actattgctt gttgcctaat acctgcgccc tcccctatat tcagcgcatg
     3181 aaagacagtc tgagcccggg tttgaccaga tcggcaacct atcaaaccac catcgacatg
     3241 cgcgcgcgcc agctcaaagc cgacgtgttt tcgacgccgt cgatgattag cctgatggag
     3301 cgcacctgca ccgagctcac cgaacctttt ttggacgaaa acgaacagac cgtcggtatt
     3361 cacatcgacg tgcgccatat ggcatcgacc cgcatcggcc aaagcgtcac catgaacgcg
     3421 gcgattatcg aagtgaaagg ccgaaaaatt cgctattcag tggatgcctt caacgacgcg
     3481 ggagtaaaga tcggcgcggg cacacaatgg cgcgcggtga tcgacaccca acgcttcgcc
     3541 ggtggcatcg agccggaggt tgatcatggg cattaggatt tcggctatcc gactggtcgt
     3601 cgctttggtg ctttggacca tcatgccggc acgcctctcc gcccaaagcg aagaaaagct
     3661 cctcgacgag atcaaccaat tacccgaggc cgagcgtcaa actaggctgg tcgccggcgc
     3721 caagaaagaa ggcacagtaa actggtatgt ggcgatgaat cgtgcctacg ccaacgatct
     3781 tgtcaatggc attcaagccg agtacccgtt tctcaaggtc aatactttga ccggcagcgg
     3841 cggatcgctg ctcaatcgag tgctcacgga aagccgcgcg cgctcgcacc aattcgacgt
     3901 cttcaacacc cgcagcatga ccgtcaacac gctaaaaaag gccggagcca tcatgcgcta
     3961 ccggtcgccg tatcgtagcg ccctgcgcga cggcttcgcg gacaaggagg gctttttgaa
     4021 tggcatcttt gccacaccgt tggtctttct gttcaatacc aagatgattc cccgcaaaga
     4081 ggcgccgagc accatcgagg atttattacg ccccaattgg accggcaaga tggccatcga
     4141 cgacgaatcc tacgactggc tggcggcatt gatcgaccac tatggcgaag ccaaaggctt
     4201 ggagctggcc gcccggctcg gccaacaaaa gctgcaagtg cgacgcggcc catcgctgat
     4261 cacccaactg gtcgccgccg gcgaattttc cttgcagatc gatagccatc accaggaagt
     4321 catcaataag aagaaagccg gcgcccctat cgactatatt ttcccccaac ctttcgtgcc
     4381 ggcgaaatct ctaataccga tctatatgtc ggcccgtccg ccccatcccc acgcggcggc
     4441 gttgctcgcc gattttctca tgtcgcgcaa aggccaggcg atcatgcacg gccatggccg
     4501 ctgggtcggc cacaagagta tagccaccaa gggtccggac gatgtcggcg agcgtaaagt
     4561 catcattcca tcggcggaaa aatggggcga ccgatacaac gaactgatcg gctggtacaa
     4621 caaattgcta ttgcgccaag gcgcctaagc ggcgtaaata aggaactatc tcgcggcccg
     4681 gcgggcgaat ccggtcggag caattaggat tgacgtatta ttgtagggtg ggtcgaggcg
     4741 cctagcgccg atacccaccg tgggcagaat gatgggtctc gctgctgctc aagccatcct
     4801 acgcgggaat cgcaccgcgc ccgagcttcg agtatcagat tgtggtaaat agcgaaactc
     4861 aatcgaggag gtcttcatga aattatttac gtaccggcct tgaccctatg cccacaggac
     4921 gaggatcgtc ctggcggaaa agaatctgcc ctacgaaacc atcgagtgcg a
//
LOCUS       DASQKS010000091         9036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4249616, whole genome shotgun sequence.
ACCESSION   DASQKS010000091 DASQKS010000000
VERSION     DASQKS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4249616"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1304)
                     /gene="metK"
                     /locus_tag="VE170_02450"
     CDS             complement(126..1304)
                     /gene="metK"
                     /locus_tag="VE170_02450"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYJ14318.1"
                     /translation="MAGKNFLFTSESVSEGHPDKLCDQVSDAILDAHLAGDPDSRVAC
                     ESLAKTGMVVVAGEITSKTKVPYVDIVREVVRDIGYTDSSMGFDGNTCAILTAIEQQS
                     PDISQGVTEGQGLHKEQGAGDQGMMFGYACDETPELMPLPIQMAHSLVHYLAKIRKGG
                     EAYFLRPDSKSQVTVEYRDGKPVKVKNVVISTQHSADVEYAKLRETIIDGVIKAVIPG
                     EYLDKDTIFHVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSAAYMARYVAKNIVAAELAQRCEVQLAYVIGVAQPVSVLVDTFGTAAIPEEKIAKIV
                     QDSFDLSPKGIILALDLKRPIYRATAAYGHFGRAPEEGLFTWEKTDRAADLKKAAGRV
                     "
     gene            complement(1382..1657)
                     /locus_tag="VE170_02455"
     CDS             complement(1382..1657)
                     /locus_tag="VE170_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HYJ14319.1"
                     /translation="MSKVVKKVEIKNKLGVHARAAALLVQTVNKFSAQINFSADGQTT
                     DGRSILGVMSLGVPQGSKIQIEATGEDAEKAVKALEKLIDARFNESE"
     gene            complement(1661..2527)
                     /gene="rapZ"
                     /locus_tag="VE170_02460"
     CDS             complement(1661..2527)
                     /gene="rapZ"
                     /locus_tag="VE170_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HYJ14320.1"
                     /translation="MANGLEIFIITGLSGSGKSFAIRALEDNGFFCVDNLPAPLIPKF
                     VELCQGYQEDIMRIALGVDLRGSQFLRALPEVLTDVRGGGHHVQVLFFDASDDVLLRR
                     FSETRRPHPSAAGGSVQDGIARERTALESIRALADKVIDTSDFNVHELKREMEQQFCD
                     APSARRMTLFLTSFGYKFGIPHDTDVILDVRFLPNPYFVNELRGKSGLDREVEQYVLA
                     NPETRAFLDRLYALLEFTLPLYEREGKSSLTLGLGCTGGRHRSVALVEELKKRFGGGS
                     LRIHVKHRDIDK"
     gene            complement(2547..3008)
                     /locus_tag="VE170_02465"
     CDS             complement(2547..3008)
                     /locus_tag="VE170_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HYJ14321.1"
                     /translation="MKITDFLSVDTVIPALVNRDKGSALEELAAGLASKHPNLEQKQV
                     LTVLQERERISSTAIGDGVAIPHGKLPGLDKIYGMFARSPAGVDFNSLDGGPTHLFFV
                     LIAPENAAADHLKALARISRLLKDESFRKHLMTACTDQELFQLISAADDKF"
     gene            complement(3131..4606)
                     /gene="rpoN"
                     /locus_tag="VE170_02470"
     CDS             complement(3131..4606)
                     /gene="rpoN"
                     /locus_tag="VE170_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYJ14322.1"
                     /translation="MAGLNLHQVQKLAQTLVMTPQLQQAIKLLQLSRVELEEMVLKEL
                     QENPALEEGATDEPEVAEPSRAETKSEPTEEFAELVAPREPNTVDKIGTLDWQEYIDS
                     HSNGVHSSLTAEAMSDDGDSPPSWENSLTKKTSLEDHLIWQLRLSKISERESNIGFYI
                     IQNISENGYLALTIDEVCSAVECTPAEAEGVLKRIQFFDPVGVAARDLRECLLVQLEN
                     LKLKDSLAARIADGYLNYLESKRYEKLAKDLGVSVDEIADAAHLIASLEPKPSRGFEQ
                     DDVRTVLPDVFVEKISGEWVIYLNDDGVPRLRVSSLYRRMAGQEGAEEEQARQYLQEK
                     VRAATWLIKSIQQRQATLMKVTQSIFKFQEEFLDHGVSHLKPMVLRQVADDISMHEST
                     VSRATANKYVHTPQGLFELKFFFQSGLMSRGGDDVASESVKEKIRAIVAGEDPRKPYS
                     DQHIAAMLSDDSIEIARRTVAKYREAMGVLPSSKRRHFIRK"
     gene            complement(4609..5331)
                     /gene="lptB"
                     /locus_tag="VE170_02475"
     CDS             complement(4609..5331)
                     /gene="lptB"
                     /locus_tag="VE170_02475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HYJ14323.1"
                     /translation="MSELKAVSVAKAYGGRRVVDGISLAVHGGEVVGLLGPNGAGKTT
                     TFHMMVGLVQPDEGQVLLNEDDLTDEPIYQRARAGISYLPQESSVFRRLTVEQNLLAI
                     LQTLNLTGVDQKERAGGLMKMLGVSHLATHKAFTLSGGERRRVEIARALVLSPFFLLL
                     DEPFTGIDPIAVAEIQKIVRNLVTSGIGILITDHNVRETLGICDRAYIVNQGTILEEG
                     SPQFIAASPKARKFYLGEGFKF"
     gene            complement(5328..5882)
                     /gene="lptA"
                     /locus_tag="VE170_02480"
     CDS             complement(5328..5882)
                     /gene="lptA"
                     /locus_tag="VE170_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HYJ14324.1"
                     /translation="MIWKILPAIVLGAFLTGSFAATRAEAAAAEGKKSVNAFEFNKKD
                     PIYITADWMEVDQAKSTIVYKGRVVAVQSDMTMRSDALIAYYDPEMKQMKQIVLDGKV
                     NATQGTRVATGDRAVFDDKAKTVTLTGNPVMRQGNNQVSGAKVVYFIEQDRAMVESDG
                     KTRVSATIFPEELKAREQGDAKSQ"
     gene            complement(5884..6645)
                     /gene="lptC"
                     /locus_tag="VE170_02485"
     CDS             complement(5884..6645)
                     /gene="lptC"
                     /locus_tag="VE170_02485"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HYJ14325.1"
                     /translation="MVALISDWGKITNQTRSLSNDEANIVPNPLQEFLRVVICIYFGE
                     LIMRKTRRAILLAVILLSLGGVAFKVAEVVQKMNIGEVKDPVAVLNALPDTVLQIKDF
                     HRAKIENGRKVWELFGDEANYLKDKREAVIKRPRFLYYDKKDETAETSAEAAHIFLNE
                     KDLERLELKGAVRVAMKGYVLTSELANYVPAKEQILLPSRTQVVGEGISIEGASMEVE
                     LEDKKIRMVRDVKTKIEPDKLAKKKSKTASPKIGG"
     gene            complement(6633..7148)
                     /locus_tag="VE170_02490"
     CDS             complement(6633..7148)
                     /locus_tag="VE170_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HYJ14326.1"
                     /translation="MKVFPVSLRRKAANIKLLLLDVDGVLTDGRIIIDDRGVESKHFH
                     VRDGHGIVLLKRSGIEVGFITGRSSPVVGQRAKELQVTLLFQGVQDKLRVYERIKKKR
                     GLSDGQIAYVGDDIVDMPVLRRVGLAVMVSDGSAELKPIADYVTVARGGMGAVREVAE
                     LILMAHNKWSH"
     gene            complement(7158..8084)
                     /locus_tag="VE170_02495"
     CDS             complement(7158..8084)
                     /locus_tag="VE170_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYJ14327.1"
                     /translation="MEAHGLLSVIDQLDENFIRAVEMMQVCKGKVVVTGVGKSGLICA
                     KIAATLSSTGTPAFYLHSGDAVHGDLGMVMPDDIVVAVSKSGETDEILKLLPHFKHHG
                     LKLIVITGNPESTLAKAGDLVLNVRVKEEACPLGLAPTTSTTVALAMGDALAVVLLEQ
                     KGFNQADFAMRHPGGILGRKLLLRVEDLMFRDARLPLVRETTSIKNALFEITAKRLGV
                     TGVTNEKDQLIGVLTDGDLRRGLEKKGEILSLPAKDLMTRNPKTIPADTLASEAVAVM
                     ENFSITSLFVLEHETKKPVGIVHLHDLIKAGL"
     gene            complement(8134..8961)
                     /gene="kdsA"
                     /locus_tag="VE170_02500"
     CDS             complement(8134..8961)
                     /gene="kdsA"
                     /locus_tag="VE170_02500"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HYJ14328.1"
                     /translation="MPTKLIQVGNVSIGGNGPLAVIAGPCVIESKESALRHAAALKQC
                     ADRVGVPYIFKASYDKANRSSADSFRGLGLEKGLAILAEVKAKIGVPVLTDVHEAAQV
                     AAVKEVADILQIPAFLCRQTDFVAAVAQSGRVVNVKKGQFLAPWDMRNVIDKILATGN
                     QQILVTERGASFGYNNLVSDMRSLVVMREMGYPVVFDATHSLQLPGGLGTASGGERKY
                     IPALARAGVATGIDALFMEVHENPDQALSDGPNSLALVNFEALISKVKEIDALVKGL"
BASE COUNT         1962 a         2612 c         2372 g         2090 t
ORIGIN      
        1 tcagtttgtc gttataatgg gttttcaaca attgctggca acacaaagag tgcgcgtggc
       61 gcaccctgct agcatattgt ggtccggaat atgaaatgat cgtcagagcg ccaagcgtgc
      121 agtcgctaca cccgcccagc cgctttcttg agatcggccg cacgatcggt tttttcccag
      181 gtaaacaagc cttcttccgg agcccggccg aagtggccat aggccgccgt agcacgatag
      241 atcggccgtt ttagatcgag agctagaatg atgcccttgg gactgagatc gaaactgtct
      301 tggacgattt tggcgatctt ctcttctgga atcgccgcgg tgccgaaggt atcgacgagc
      361 accgaaaccg gctgcgccac accgatgaca taagccagct gtacttcgca gcgttgggcc
      421 aactcggcag cgacgatgtt cttcgcgacg tagcgcgcca tataagcggc gctgcggtcg
      481 accttcgacg ggtccttacc cgaaaaggcg ccgccgccat ggcggcccat gccgccatag
      541 gtgtcgacga taattttgcg accggttaga ccgcaatcgc cctgcgggcc gccgacaacg
      601 aaccggcccg tcgggttgac gtgaaaaatt gtgtctttgt ccagatactc gcctggaatc
      661 acggctttga tgacgccgtc gatgatcgtc tcgcgcagtt tggcgtactc gacatcggcg
      721 ctgtgctggg tcgagattac gacgtttttc actttgaccg gcttgccgtc acgatactcg
      781 acggtaacct gggatttgct atcgggacgg agaaaataag cttcaccgcc tttccgaatt
      841 ttggcaagat aatgaaccag gctatgcgcc atctgaatcg gcaacggcat cagctccggc
      901 gtctcgtcgc aggcataacc gaacatcatg ccttgatcgc cagcgccctg ttccttgtgc
      961 agaccttggc cttcggttac cccctgagaa atatccggcg actgttgctc gatggcggtg
     1021 agaatcgcac aggtgttgcc atcgaaaccc atcgacgaat ccgtgtagcc gatatcgcgc
     1081 actacttcgc ggacgatatc gacataagga actttggtct tactggtgat ttcaccggca
     1141 acgacaacca tgccagtctt ggctaagctc tcgcaggcga cgcggctatc ggggtccccg
     1201 gcaaggtgag cgtcgagtat ggcatcggat acttgatcgc ataacttatc cggatgccct
     1261 tccgaaacgg actccgaagt gaataagaaa tttttgccgg ccatcagcga gacctccaat
     1321 gttctaggaa tcgaaaatta caatctgaga atattatcgg atcgtgggta gcgatacaac
     1381 cttattcgct ctcgttgaag cgcgcatcga tgagcttttc gagcgctttt accgcctttt
     1441 cggcatcttc gccggtggcc tcaatttgaa tcttgctgcc ctgcggaacg cccaaggaca
     1501 tcacccccaa aatgctccgt ccatcggtag tttgcccgtc agcggaaaaa ttgatttgag
     1561 cggaaaattt gtttacggtt tggaccagca gagcggctgc gcgcgcatgc actcccaatt
     1621 tattcttgat ttcgactttc ttgacaactt tgctcataag ttatttatcg atgtcgcggt
     1681 gtttgacatg aatgcgcaag ctgccgccac cgaaacgctt cttcagttct tccaccagcg
     1741 ccacggaacg gtggcggccg ccggtgcatc cgagccccag agtcaagctg cttttgccct
     1801 cacgttcgta cagcggcaag gtaaactcca agagcgcata aagacggtcg agaaaggcgc
     1861 gagtttccgg attggccagg acatactgct ccacttcccg gtcgaggccg ctcttaccgc
     1921 gcaactcgtt gacgaaatac ggattgggca ggaagcgcac gtcgaggatc acatcggtgt
     1981 catggggaat cccgaactta tatccgaaag aagttagaaa caacgtcatg cggcgcgccg
     2041 atggggcatc gcaaaattgc tgctccattt cgcgcttgag ctcatgcaca ttgaaatcgc
     2101 tggtatcgat cactttgtca gccaaagcac gaatcgactc caaggcggtg cgctcccgcg
     2161 caataccgtc ctgaacggaa ccaccggcgg cggaggggtg aggccgccgg gtctcgctga
     2221 aacggcgcaa caacacgtca tcggaagcgt cgaaaaacag cacttgaacg tgatgaccgc
     2281 cgccgcgcac atcggtcaac acctccggca atgctcgcag aaattgactg ccgcgcaaat
     2341 cgacccccaa ggcgatgcgc atgatgtctt cctggtaacc ttggcaaagc tcgacgaatt
     2401 tcgggattag cggcgccggt agattatcga cgcaaaaaaa gccgttgtct tcgagagcgc
     2461 gtatcgcaaa gctcttaccg gaaccggaaa gaccggtgat aataaagatc tctaagccgt
     2521 tggccatgct agaatttgaa ttagaattag aatttgtcgt ctgccgcgct aatcagttgg
     2581 aacagctcct ggtctgtgca agcagtcatt aaatgcttgc ggaaggattc gtccttcaac
     2641 aagcgtgaaa tgcgtgccag cgcttttaag tgatcggcgg cggcattctc cggagcgatc
     2701 aggacaaaaa acaggtgagt tgggccgcca tcgagagaat tgaagtcgac gcccgccgga
     2761 ctgcgcgcaa acatgccata gatcttatct aagccaggta gtttgccatg gggtatcgcc
     2821 acgccatccc cgatcgccgt cgaactgatc cgttcccgct cctgaagcac ggtgagaact
     2881 tgtttttgct ctaagttcgg gtgcttggag gctaacccag cggcgagttc ttccaacgcc
     2941 gagcctttgt cgcggttgac gagcgcgggg atgacggtat ccacactgag aaaatcggta
     3001 atcttcacaa acttttcctt ttgtcgccga gccgcggtgg ctctgattat tgcacagcca
     3061 gaatcggctc ggcttggcga aatgcaacag gacttgaatg ctggtcatcc ccggctcaat
     3121 tcctatccgg ctacttgcgg ataaaatggc gccgcttgga cgacggcagg acgcccatgg
     3181 cttcgcggta cttagcgacg gtgcggcggg cgatttcgat ggaatcgtcg gatagcatcg
     3241 cggcaatatg ctggtcgctg taaggcttgc gcggatcttc gccggcaacg atcgcgcgaa
     3301 ttttttcctt gacgctctcg ctggcgacat cgtcgccgcc ccggctcatt aagccgctct
     3361 gaaagaaaaa cttgagctcg aacaatcctt ggggagtatg gacgtattta ttcgccgtcg
     3421 cccggctcac cgttgattcg tgcatagaaa tatcatcggc gacctggcgc aacaccatgg
     3481 gttttaaatg gctaacgcca tgatcgagga actcttcctg aaatttgaat atgctctggg
     3541 tgaccttcat cagcgtcgcc tggcgctgtt gaatgctctt aatcagccag gtcgccgccc
     3601 gaaccttttc ctgcaggtac tgccgcgctt gctcttcttc ggccccttct tgcccggcca
     3661 tgcggcgata gagcgagctc acgcgcagcc gcggcacgcc gtcgtcgttt agataaatca
     3721 cccattcgcc gctgattttc tcgacgaaaa catcgggcag cacggtgcgc acatcgtctt
     3781 gctcgaaacc acgggacggc ttcggttcca gcgaagcgat caaatgcgcc gcgtcggcga
     3841 tctcgtcgac ggagacgccg agatctttcg cgagcttttc atagcgcttc gactcgagat
     3901 aattcaggta accgtcggcg atgcgcgccg ccaggctatc cttgagcttt aggttttcca
     3961 gttgcaccag aagacactcg cgcaggtccc gcgcggcgac ccccaccgga tcgaaaaatt
     4021 ggatgcgttt gagcacccct tccgcttccg cgggagtaca ttccacagca ctgcaaactt
     4081 catcgatggt tagagcgaga taaccattct cactgatatt ctggatgatg tagaagccta
     4141 tattcgattc ccgttcgctg attttcgaca aacgcaactg ccaaatcaga tgatcttcca
     4201 aggaagtttt cttagttagg ctattttccc acgacggcgg agaatcgcca tcgtcggaca
     4261 tcgcctcggc ggtgagcgag ctgtgcacgc cgttggaatg gctatcgata tactcttgcc
     4321 aatccaacgt gccgatctta tccaccgtgt tgggctcgcg gggcgccacc aattcggcga
     4381 actcttctgt cggttcgctc ttggtttcgg cgcgcgatgg ctcggcgact tcgggctcgt
     4441 ccgtggcgcc ctcttcgaga gctggatttt cttgcagctc cttcagaacc atctcttcga
     4501 gctcgacgcg ggacaactgc aataacttga tcgcctgctg taactgcggt gtcatcacca
     4561 gcgtctgagc cagcttctga acttgatgta gatttagacc tgccatggtt agaacttaaa
     4621 gccttctccc aaataaaatt ttcgtgcttt ggggctggca gcaatgaatt gcggcgagcc
     4681 ctcctccaag atcgtccctt ggttgacgat gtaagccctg tcacagatac ccaacgtctc
     4741 tctgacgtta tgatcggtga tcaaaatgcc gatgccgctg gtaacgaggt tgcgaacgat
     4801 tttttggatc tccgccacgg cgatgggatc gatgccggta aaaggttcgt cgagcaggag
     4861 aaaaaatggc gagagcacca acgctctggc aatctcgacg cgccgccgtt cgccaccgga
     4921 taaggtaaac gccttatgcg tcgcgaggtg actcacgccg agcatcttca tcagcccgcc
     4981 ggcccgttct ttttggtcga caccggtcaa attgagagtt tgcaggatcg ccagcaaatt
     5041 ctgctcgacc gtgagacggc gaaataccga ggattcctgc ggcaggtagc ttatcccggc
     5101 gcgggctcgc tggtagatcg gttcgtcggt gagatcgtct tcgttcaata acacttgccc
     5161 ctcatcgggc tgaactaaac ccaccatcat atgaaacgta gttgtcttac cggccccgtt
     5221 ggggcctaac aaaccaacca cttcgccgcc gtgcaccgcc aagctgatgc catcgaccac
     5281 ccgtcggccc ccgtaggctt tggcaaccga cactgctttg agctcgctca ttgggacttg
     5341 gcatctccct gctcccgggc cttcaactct tctgggaata ttgtcgccga gactctcgtc
     5401 ttgccgtcgc tttcgaccat ggcgcgatct tgctcgataa aatagactac cttcgcacca
     5461 ctgacctgat tattgccttg gcgcatcacc gggttgccgg taagggtgac cgtcttggct
     5521 ttatcgtcga aaacagctcg atcgccggtg gcaacacgcg tgccttgagt tgcattgact
     5581 ttgccatcca ggacgatttg tttcatctgc ttcatctcag gatcgtagta agcgatgagc
     5641 gcgtcactgc gcatggtcat gtcggactgc acggcaacaa cccggccttt gtaaacgata
     5701 gtgctcttcg cctgatcgac ttccatccag tcggcggtga tgtagatcgg atccttttta
     5761 ttgaattcga aggcgttgac cgactttttc ccctccgccg ccgccgcctc ggctcgcgtg
     5821 gcagcaaagg aacccgtcaa gaatgctccc aatacgatcg ccgggaggat tttccagatc
     5881 atgtcatccc ccgatctttg ggctggcggt tttggatttt ttcttggcaa gtttatccgg
     5941 ctcgatctta gtcttgacgt cacgcaccat acgaatcttc ttatcctcca attccacttc
     6001 catgctcgca ccttcaattg aaatgccttc gccaacaacc tgagttcggc tcggtaacaa
     6061 gatttgctcc ttggcgggca cgtagttggc caattccgaa gtcagcacgt aacccttcat
     6121 agcgacccgg accgcgccct tgagttccag cctctcgaga tccttctcat tcaggaaaat
     6181 atgcgccgct tcggctgagg tttccgccgt ctcatccttt ttatcgtagt agagaaagcg
     6241 cggccgcttg atcaccgctt ctcgcttgtc tttgaggtaa ttcgcctcat cgccgaaaag
     6301 ttcccatacc ttgcggccat tttcgatctt ggcgcgatga aaatccttga tttgcaaaac
     6361 ggtatccggc aaagcattga gcacagcaac gggatctttt acttcaccta tgttcatctt
     6421 ctggactact tcggcgacct tgaaagccac cccccctaga gagagcaaaa tcaccgcaag
     6481 taagatcgcc cgtcgagttt tacgcataat cagttcacca aagtagatgc aaattaccac
     6541 tcttaggaat tcctgtaaag gattaggcac gatatttgct tcgtcattgg ataagcttct
     6601 ggtctgattg gtaattttgc cccaatcaga aattagtgcg accatttatt atgggccatt
     6661 agaatcaact cggcgacttc gcgtaccgcg cccataccgc cccgagccac ggtaacgtag
     6721 tcagcaatcg gcttaagttc tgccgaacca tcgctaacca ttaccgccaa acccaccctc
     6781 cgcagcaccg gcatatcgac gatatcgtcg cccacatagg caatctgccc gtcggacaga
     6841 ccgcgcttct ttttgatccg ttcatagacc cgcagcttat cttgcacacc ctggaatagc
     6901 agcgtgacct gcaactcctt ggcgcgctga ccaacgacgg gagacgagcg tccagtgata
     6961 aaacccacct caatgccgct gcgttttaaa agcacgattc catgaccatc gcgaacatga
     7021 aaatgtttgc tctcgacacc gcggtcatcg atgatgatcc gcccgtcggt gagcacaccg
     7081 tcgacgtcca acagcagcaa ttttatgttc gccgcctttc ggcgtagcga gacgggaaag
     7141 actttcacgg tgagacgcta gaggccggct ttgattagat cgtgcaaatg aacgataccg
     7201 accggtttct tggtctcatg ctcgagcaca aataatgaag ttatcgaaaa attttccatg
     7261 accgcaactg cctcgctggc taaagtgtcc gcgggaatag ttttgggatt gcgcgtcatt
     7321 aaatcctttg ccggcaagga gagaatttcc cccttcttct ccaaccctcg ccgtaaatca
     7381 ccgtcggtta gaacaccgat gagctgatct ttttcgttag ttactccggt gacaccgagg
     7441 cgcttcgcgg tgatctcaaa aagcgcgttt tttatgctgg tggtctcccg aaccaacggt
     7501 aagcgcgcgt cgcgaaacat caaatcttca acccgcaaga gcagcttgcg gccgaggata
     7561 ccgccgggat ggcgcatggc gaagtccgct tgattaaagc ctttctgctc gagtaaaact
     7621 accgcgaggg cgtcgcccat ggccagcgcc accgtggtgc tggtggtcgg cgccaagccg
     7681 aggggacagg cctcctcctt aacgcgaacg ttgagcacca agtcacccgc tttcgcgaga
     7741 gtcgactcgg gattgccggt gatcacgatc agcttaaggc catggtgttt gaaatggggc
     7801 agcagcttga ggatctcgtc ggtctcgccg cttttggaaa ccgcaaccac gatatcgtcg
     7861 ggcatcacca tgccgagatc gccgtgcaca gcgtcgccgg aatggagata aaacgccggc
     7921 gtgccggtgc tcgacaaggt cgcagcgatt ttggcgcaga tcaggcccga tttgccgaca
     7981 ccggtcacga caaccttgcc cttgcaaact tgcatcatct cgaccgcgcg gatgaaattt
     8041 tcatccagct gatcgatgac ggacaacagc ccgtgagcct ccatccgcaa aacctcggcg
     8101 gcacgactta gaacggtttg cttgttcacg gtatcaaagc cctttgacca gagcgtcgat
     8161 ctccttaacc ttgctgatca aggcttcaaa gtttaccaac gccaaagaat tgggcccatc
     8221 gctcaacgcc tgatcgggat tttcatgcac ttccatgaat agcgcatcga tgccggtggc
     8281 cacaccggcg cgcgccaggg cggggatata ctttcgctcg ccgccggaag cggtgcccaa
     8341 tccgcccggc aactgcaaac tgtgtgtcgc atcgaagacc acgggatagc ccatctcgcg
     8401 catcaccacg agggagcgca tgtcggaaac taagttatta taaccaaatg acgcgccgcg
     8461 ctcggtgaca aggatctgct ggttaccggt ggcgagaatc ttgtcgatga cgttgcgcat
     8521 gtcccagggc gcgagaaatt gccccttctt gacgttgacg actctgccgg actgggccac
     8581 agcggcaacg aaatcggtct ggcggcacag gaacgccggg atttgcagga tgtcggcgac
     8641 ttctttgacc gccgccactt gcgcggcttc atggacatcg gtgagcaccg gcacgccgat
     8701 ctttgctttg acctcggcca gaatcgccaa gcccttttca agaccaagcc cacggaacga
     8761 atcggccgag ctgcgattgg ccttatcgta ggaggctttg aaaatatacg gcacgccgac
     8821 gcggtcggcg cattgtttga gcgccgcagc gtggcgcagc gccgattcct tgctctcgat
     8881 cacgcacggt ccggcaatga ccgccagcgg gccgttcccg ccgatcgaaa catttccaac
     8941 ttgtatcagc tttgtgggca tcggttcggt caagtgccga ctaagtgcgg acaatcttca
     9001 tgcgcggcgc atccgccagt gagcgccggt tttgca
//
LOCUS       DASQKS010000092         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5509971, whole genome shotgun sequence.
ACCESSION   DASQKS010000092 DASQKS010000000
VERSION     DASQKS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5509971"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VE170_02505"
     CDS             complement(<1..817)
                     /locus_tag="VE170_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14329.1"
                     /translation="MANPKLRFYFREPTIDTNIPITDGTVKIDGFDWEFVPTEEECDA
                     WDCGFAARVRAYAKGEAHISIPAFPNRKFRLAYIYVNSKAGIESAKELEGRRVGIMQW
                     DNTAGVWARGALQHYYGVDLKKINWCSPRAKKMALADGIRIEQIDSNDDPDLLLDNML
                     LDGKIDAVIGPNVLPSITARDPRMRRLFRDFRKEEQEYYRQTKIFPTSHIVTLKQAFV
                     EKHPNAPVALLKAFRKSRDVAFDRLVGPDPQVIAYSWIAAAVAEQRELMGDDYW"
     gene            1048..2202
                     /locus_tag="VE170_02510"
     CDS             1048..2202
                     /locus_tag="VE170_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14330.1"
                     /translation="MARQYKCISGDGHIDLNPDIWRDRVQAKYRERAPKRVKMPNGSD
                     AVVIDGGKPNTIGITRSVRVAHTDLAKQVPTFETCAGTGSPQQRLAEQEQDGIDCEIL
                     FSQITFVLRQTKDDEFYRDCIRAYNEFLGEEYSAPAPDRLVCMGTLPTTGVDDAIKEM
                     EHCAKLGMKGVKLDRYPSGKSYPTATDDKFWAAALDLRMALTNHNTGNMGSGKGEPDY
                     LYAKDPGHDVHQKDAFKFFTRFTNDAMTAPLQMAFAGVWDRFPKLELYWAETMVGWFE
                     YGLWQVDDHYRRYIGMIHDNWGLPKLERMPSEYLKDRNYWGFLHDPVGVKRRHSIGVD
                     KIMWGTDFAHAASEYPNSQKHLEEDFAGVPENERRAILVDNCVRYFHLND"
     gene            2319..3341
                     /locus_tag="VE170_02515"
     CDS             2319..3341
                     /locus_tag="VE170_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14331.1"
                     /translation="MTDVGTTHEDLEHFLGDHYLIGAGLKRDPRVKPRSGKTAAHWQW
                     DGIYRGLQRSGEIVTVGPDGMTGMRSVVGIEAKNFPIWMNAQILMPGERTQCHRNLRS
                     ETRLVCEAPKDAVFVCEYEAYPMERGDVVISPAWTYHDHWNKDKTPAIWIDGYDNGYN
                     PNVNINERFPKDNPYEEVKKPAGYGQRTLGLARAITSEAPFPLPPMRYPWADTHAALM
                     ALRENNESDPYDGVMIMLASPVDGGPTLPTIAWQAQLISKKQKTMAHRHNSTTFYFAF
                     EGAGAVIIDGERLEYNKGDIFAVPAWSWHHHENSNGDDTILFSIDDWPAMRKLGFYMK
                     AEAQGF"
BASE COUNT          800 a         1066 c          937 g          668 t
ORIGIN      
        1 cccaatagtc gtcacccatc agctcacgtt gctcggcgac cgcagcggcg atccaggaat
       61 aggcgatcac ttgcgggtcg gggccgacga gacggtcgaa ggcgacgtca cgcgacttgc
      121 ggaacgcctt gagcagcgcc accggcgcat tgggatgttt ctcgacgaat gcttgtttaa
      181 gcgtcacgat atggctcgtc gggaatattt tcgtctggcg gtaatactcc tgctcttcct
      241 tgcggaagtc gcgaaacagc cggcgcatgc gcggatcccg cgccgtgatc gacggcagca
      301 cgttggggcc gatgaccgcg tcgatcttgc cgtcgagcag catattgtcg agcaacagat
      361 ccggatcgtc gttcgaatcg atctgctcga tgcgaatgcc gtcggccaga gccatcttct
      421 tcgcccgcgg cgagcaccag ttgatctttt tcaaatcgac gccgtagtaa tgttgcagcg
      481 cgccgcgcgc ccacacgccg gcggtattgt cccactgcat gatgccgacg cgccgtcctt
      541 ccagctcctt cgccgattcg atgccggctt tgctgttgac gtagatgtac gccagccgaa
      601 acttgcggtt gggaaaagcg gggatcgaaa tatgcgcctc gcccttcgcg taagcccgca
      661 cccgggcagc aaagccgcag tcccaggcgt cgcattcctc ttccgtcggc acgaactccc
      721 agtcgaaacc gtcgatcttc accgtgccgt cggtgatcgg aatattggta tcgatggtcg
      781 gttcgcgaaa ataaaagcgc agttttggat tggccatggc ggctcctttc gtaactggcg
      841 caacataacg cgacatagcg ctgcggttca atcgcagagt ggcaaaattg gagtaatgaa
      901 gcactggggc ggtgcaatga tagttccgag attcctgaac ccgtcacttc aatacttcct
      961 tctgctccat ttccacttgc ttgacaccct ttgctaaact gcatagttaa tctaagcaca
     1021 aactacgcac tcgaaaggac tcgccccatg gcaaggcaat acaaatgcat ttccggcgac
     1081 ggccacatcg atttgaatcc cgacatctgg cgcgatcgcg ttcaagcgaa gtatcgtgag
     1141 cgcgcgccca agcgcgtcaa gatgcccaac ggctccgatg ccgtggtcat cgacggcggc
     1201 aaacccaaca ccatcggcat cacccgcagc gtgcgcgtcg cccatacgga tctcgctaag
     1261 caagtgccga ccttcgaaac ctgcgccggc accgggtcgc cacagcaacg gttggccgaa
     1321 caagaacagg acggtataga ttgcgaaatt ctcttctcac agatcacctt cgtcctgcgt
     1381 caaaccaagg acgacgaatt ttaccgcgac tgcattcgcg cctataacga attcctcggc
     1441 gaagaataca gcgcgccggc acccgaccgt ttggtttgca tgggcaccct tcccaccacc
     1501 ggcgtcgacg acgcgattaa agaaatggag cactgtgcca agctcggcat gaaaggcgtc
     1561 aagctcgatc gctatccgag cggtaagagt tatcccaccg ccaccgacga caaattctgg
     1621 gccgccgccc tggacctgcg catggcgctg accaatcaca acaccggcaa catgggctcc
     1681 ggcaaaggcg agcccgatta tctctatgcc aaggatcccg gccacgacgt ccaccaaaaa
     1741 gacgcattta agttctttac ccgcttcacc aacgacgcca tgaccgcgcc actgcagatg
     1801 gcctttgccg gcgtctggga tcgctttccc aaattggaac tttactgggc tgaaaccatg
     1861 gtcggctggt tcgagtatgg cctgtggcag gtcgacgatc attatcgccg ttacatcggc
     1921 atgattcacg acaactgggg actgccgaag ctcgaacgca tgcccagcga atacctgaaa
     1981 gaccgcaact actggggctt tctacacgac ccggtgggcg tcaaacggcg tcatagcatt
     2041 ggcgtcgaca agattatgtg gggcaccgac ttcgcccacg ctgccagcga gtatccaaac
     2101 tctcagaaac acctcgaaga agatttcgcc ggcgtgccgg aaaacgagcg gcgcgcgatc
     2161 ttggtggata attgcgtgcg gtattttcac ctaaacgatt gagcgatccc gtttggaacg
     2221 attggaaccg ctggaactac tggaacacgt tgcgaaaatt ccaaaagttc caatcgttcc
     2281 aataggcttt aaaagccccg ctaagattag gagataagat gactgacgtc ggcaccaccc
     2341 acgaggacct cgaacacttt ctcggcgacc attatttgat cggcgcgggg ctgaaacgcg
     2401 acccgcgtgt caagccccgg tcaggcaaaa cggcggccca ttggcaatgg gacggcattt
     2461 accgcggtct tcaacgttcc ggcgagatcg tcacggtcgg gccggatggc atgaccggca
     2521 tgcgttcggt ggtcggcatc gaagcgaaaa acttcccgat ctggatgaac gcgcaaattc
     2581 tcatgcctgg cgagcgcacc caatgccacc gcaacctgcg cagcgaaact cggttggtct
     2641 gcgaagcgcc caaggacgcg gtatttgtct gcgaatacga ggcctatccg atggagcgcg
     2701 gcgacgtcgt catcagtccg gcgtggacct accatgatca ctggaacaaa gacaaaacac
     2761 cggcgatctg gatcgacggt tacgacaacg gttacaaccc caacgtcaac atcaacgagc
     2821 gctttcctaa agacaatccc tacgaagaag tgaaaaaacc cgcgggctac ggccagcgca
     2881 ccctcggctt ggcgcgcgcg atcaccagtg aagcaccttt tccgctaccg cccatgcgtt
     2941 atccctgggc cgacacccac gcggcactga tggcgctgcg cgaaaacaac gagagcgatc
     3001 cctacgacgg cgtgatgatc atgctcgcca gcccggtgga cggcgggcct accttgccga
     3061 ccatcgcttg gcaagcgcaa ttgatatcga aaaaacaaaa aaccatggcg caccgccaca
     3121 acagcaccac cttctacttt gccttcgaag gcgccggcgc ggtgatcatc gacggcgaaa
     3181 ggctggaata caacaaaggc gatatcttcg ccgtgcccgc ctggagctgg caccatcatg
     3241 agaacagcaa cggcgacgac acgatccttt tctcgatcga cgactggccg gcgatgcgaa
     3301 agcttggctt ctacatgaaa gcggaagccc aggggttctg aaagccggcg ggttgcaacc
     3361 cgccggctca atttggattt caatcgccag ctatcaattg tcccgccgtt tattcgcagc
     3421 ggcgaacatt aatccgcccg ccgaatcccc cctagagtac cgcgcaactt t
//
LOCUS       DASQKS010000093        11957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5509986, whole genome shotgun sequence.
ACCESSION   DASQKS010000093 DASQKS010000000
VERSION     DASQKS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5509986"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VE170_02520"
     CDS             <1..601
                     /locus_tag="VE170_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ14332.1"
                     /translation="DMSKPENIKKFVDDAAKHFGRVDVVVNNAGGPPPGEFMKFTDED
                     WQNAFNLSFMSTMRMTREAVPHLRKVGGGKVINITSYSVKEPIVGLVLSNAVRSAVIG
                     LAKTLSRELARDNILFNNVCPGRIDTDRAQKLNKARADRLSRPVEEINKEMAAEVPLG
                     RYGTAEEAGDLIVFLGSDRASYITGTTIAIDGGLVRATQ"
     gene            614..937
                     /locus_tag="VE170_02525"
     CDS             614..937
                     /locus_tag="VE170_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14333.1"
                     /translation="MVHFVIQWDLPDQKANLTVYANKAKNDWLPITLGHKGVIEVRNY
                     RNPLEVTPHVAITIDFDTLDNWRDFIDSADYIRIMRELRVLGCRNFTVNIWAPSKYFP
                     ESMKP"
     gene            complement(1121..2353)
                     /locus_tag="VE170_02530"
     CDS             complement(1121..2353)
                     /locus_tag="VE170_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14334.1"
                     /translation="MAQAVPKGYFAKNIDALGYHDLNGKPAFKLAMQEKNRRWYLYVA
                     HLWHRGWSILDVTHPEAPQFCTYVAGPENTWTLQIQLADGKMIAGLERIPPGWGGAEG
                     QPCAEGFVIFDVSEPTEAKQIGHWETGSTGTHRNFYDGGNLVHAAAGASGLTGKIYRS
                     VEISDPSNPREIGRFSLPEQETGAETMGLKFSLHGPAHIEAERAYLSYGDGGAVILDV
                     SDLAQPKLVSRLAFRGITATQGIHTYLPLPRRKLALINDEAIKENGDENLNLAGIVDL
                     RDEKRPRMLSLFPLPEPPSESELKNFYEKAGRFGPHNHHHPNHQTCLEDRDDVAYLTY
                     FNAGLRVYDIRDARQPREVGYFVPADPTLRIGTKPSRPVAQSEDVLVDRRGFIYLSDK
                     THGIHILRVNQIWPYQNG"
     gene            complement(2356..3012)
                     /gene="nth"
                     /locus_tag="VE170_02535"
     CDS             complement(2356..3012)
                     /gene="nth"
                     /locus_tag="VE170_02535"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYJ14335.1"
                     /translation="MTEREMHTAVRILRREVPKWETPVVTLMAETYQSPFRVLISCIL
                     SLRTQDATTAKASHRLFAIADTPEAMVKLTAKKIEKLIYPVGFYKTKARQILEICRTI
                     IDRHDGKVPDEIDELLKFKGVGRKTANLVVTLGYNKPGICVDTHVHRISNRWGYIQTA
                     TPEKTEVALRAKLPKQYWIEYNNLLVNFGQHRCRPISPVCSDCPVKKYCPQLGVGVRR
                     "
     gene            3093..3668
                     /locus_tag="VE170_02540"
     CDS             3093..3668
                     /locus_tag="VE170_02540"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYJ14336.1"
                     /translation="MVADKLVSALISRTPEIIAGDGLRPAAVLILIQERADGDYIVLT
                     KRGDNMPTHKGQIAFPGGRAHPGDADLVATALRESHEEIGVVPEHVRVLGRLDEFTAG
                     YGMVVTPVIGVIPPQYEFLIDLAETSAVASVPICSLMEPSTYVKNAHLSPGGHPSYHF
                     YINNGWDVWGVTARILVQFLELAYDFESEKK"
     gene            3649..4110
                     /locus_tag="VE170_02545"
     CDS             3649..4110
                     /locus_tag="VE170_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14337.1"
                     /translation="MSLKKNSLGLMLAGALGLFSGCAAGPHFYVQYGDEYREISLEKL
                     LADNPLAPGENIRVANLGRSASASQHIVQIRDREILHVHKLHDGTVIILRGHGYLVLA
                     GQRIVLKAGDVIHIPRGVAHQYINGAGEPTVVLAVYAPAFDGKDTHPVAVP"
     gene            4138..5652
                     /gene="lnt"
                     /locus_tag="VE170_02550"
     CDS             4138..5652
                     /gene="lnt"
                     /locus_tag="VE170_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYJ14338.1"
                     /translation="MKLTIILLPMLAAALLAAPFLEPWLFPLAWVAFVPLFWAIDRAE
                     SSRRAILYGWLMGLTAHLIGFHWLTYTISVFGGLPFAVSCVVFLLYAALQGIQLALFA
                     LLVRRLGVGPLLLFPALFWVSLEFLFPLLFPWYIANSQVAFPWVIQTADLVGPYGTGF
                     IVIWFSAALYRALFTSAEPSKRILPLAYACLAIVVALAYGFQRLQSVGDEMASARKLS
                     VGTVQGNVDIDMKWNPVLAQKNLDQHRQLTTELDAVPLVVWPESAIEAIIPETLQALP
                     LSVMPAFKSERAYFIFGAKSFRGNPGQPGGKAFNTAFFTDAKGRILARYHKQVLLAFG
                     EYLPFAKLLSWIPALPMADGFTPGPGPVVFHLPRAVRVAPLICYEDLLPDVVRRFVGQ
                     TGANVLINLTNDAWYGRSVGPWQHLRLAQSRAIETRRSLLRVTNTGVTSLVNAKGELV
                     KTLPMFTAAVMHAEVDVLNETTYYVRFGDWFAWVMTIATFALILLHAKRSWLRR"
     gene            complement(5858..7078)
                     /locus_tag="VE170_02555"
     CDS             complement(5858..7078)
                     /locus_tag="VE170_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYJ14339.1"
                     /translation="MSELYPYPRFSLAERDRRWNAVRVKMAEQHIDVIVTPQNTGHSM
                     DFQANTRYLTHCGGGGDADIAAVFPLNGAVTAIATSANPRWTTTQDWTTDVREARREY
                     GKVIVERLKELTLDHGRIGITGLGEVEGTRTPEGTILHGTWQRIRDAFPNAQLVDATP
                     ILVEVRYLKSAEEIQVLTHSMAIVEEGYKAEIEAARPGVRDWDVWAAAQYGMMRNGSE
                     MPVHCNWISGKNPVRTLTRPSMRLLEHGDLIINELEANWVGYRAQGVQPVFVGAVNPV
                     HQELIKVQREIFNRVMEHLKPGVTVKELAELTIRTAESVAPKSGPAAGARGDLNMHGR
                     GAGDDGPIITAHAKSPRQLGVTLQENMVYIFKPSAITADGQSLSTWGDTIVVTANGGK
                     RLGKRPHDLAVSGG"
     gene            complement(7369..8559)
                     /locus_tag="VE170_02560"
     CDS             complement(7369..8559)
                     /locus_tag="VE170_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYJ14340.1"
                     /translation="MAFEKPIIPTFSMKERDRRWSLLRAAMKQADFDALIALPHEGHW
                     DQFGCDTRYVTQIGGTQTEVGCVVPLEADVTAVVRGENEIEWWGLAQDWVTDIRPSRR
                     SYGEPVIDRLKEVRAVRVGVVALSGMVRAPEGVVPWGTFEKIRTALPNVKFANATDVM
                     QEVRSVKSAEEVSFIEKAAEIIGHANEVLIQHAKVGVGEHELIAEMLREIVRRGGEPI
                     TMMLFGTGGPEVPWAQRVFTTRKLKAGDLINTEVEGKYAGYIAQALQPISLGPKPKEL
                     EKIFDATKVIFDRMVKFLKPGISFGAVAKFYQDEVAGAGYEPDGALMHGRGLGEDAPM
                     LWGARKDFPEKDQLLKEGHVFILKPACKRGFMRDSIRAGDTVAIEANGARRLGKRDLT
                     FVTV"
     gene            complement(8697..9593)
                     /locus_tag="VE170_02565"
     CDS             complement(8697..9593)
                     /locus_tag="VE170_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14341.1"
                     /translation="MFRAETLDHVAFPVRDLPRSEKFYLDTVGLTFLTQRKNADGSPR
                     HTYVKAGENIVGLTLPGVAVPASASGAPRYALAMPDESSFKAAVERIRASGVKCFDVE
                     SHPTDSPFVETFQFIDPDDNQIELCVRRLPLAAKDGISHVIFETANLARATRFYTEGL
                     GLKPVGTVRGETLFEFQNGQMVGIKEVKELSERTKKKGRACHVAFNVSQEDYDVMLER
                     IPKLEGRSLGDFRASDGLRPPGERSVYFFDPDTNYLQITALGEEDWSMISDEEKWQKT
                     IENRHKQGRGISSFDKGMKIQK"
     gene            complement(9606..10700)
                     /locus_tag="VE170_02570"
     CDS             complement(9606..10700)
                     /locus_tag="VE170_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14342.1"
                     /translation="MRFPLKLAVATAILAACFAGVTASAQTSEDLWRGLEKLPAADRE
                     KKLLEGAKREGEMVWYTNSGIENATRYIQGFKKKYPSINANFWRSKTRQVTQRIVAEA
                     NAGKHLVDVIKPSTDLLPILLERNLIGKYDTPLRAIYPAHAKSPFYTNMNYAFRVFSF
                     NPRKVNAKDAPKSWDDLLQPKWKNEILFDESSLEEVMAMLAVWGKERTVNYLNKLTQQ
                     NLMIRVGRDTTTQMMMAGEGNLAVTTYAYNNEGLRATNAPVDWVAEDLIPALIYPLTL
                     ARNAPHPHAAALFYDYLISEEGQRLIAREGRVVAHPKVEPIYPRMKELQNLLGTPRVQ
                     LNTLEQNAKLLKDGVQILDEVVLKRKSSGH"
     gene            complement(10981..11913)
                     /locus_tag="VE170_02575"
     CDS             complement(10981..11913)
                     /locus_tag="VE170_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14343.1"
                     /translation="MKIMVVGSSGYIGRHVVKNLANQGAEVVAFDLVAAPPQWSVPGV
                     PFVQGNMTNLEEVMAVVLDNGIQRMVALGYYMTPLLAPECRDLLNAARVNIIGVTNLF
                     EAARLAKMQRIIFASTVGIFGYQDGYGSEPIDEHTEPRAPKSLYGLMKLVNNGLAERY
                     SKTYGVQIVKVHSAAVIGPGNTAFSKRMIQDPALGNPGFANWPSNGPRNIVGVGDIAQ
                     LYTKLALAEEVKQETYMGTGPSPTGNEVAGIVKKYLPNAEITFDEKARVPAWNFDNSK
                     AVNEFGWKIQSVEKMVLDEINGTREAAGLSRVGI"
BASE COUNT         2241 a         3343 c         3554 g         2819 t
ORIGIN      
        1 ggatatgagc aagccggaga atatcaagaa gttcgtcgac gatgcagcga aacattttgg
       61 ccgggtcgat gtggtggtca acaacgccgg cgggccccct cccggcgagt tcatgaagtt
      121 taccgacgag gactggcaaa acgctttcaa tctcagtttc atgagcacca tgcgcatgac
      181 ccgcgaagcg gtgccgcatc tgcgcaaagt cggcggcggc aaagtgatca atattacttc
      241 ttactcggtc aaagagccga tcgtcgggtt ggttctgtcc aatgcagtgc gtagcgcggt
      301 gattggcttg gccaagacgc tgtcacggga actggcccgc gacaatattc ttttcaataa
      361 cgtttgcccg ggccgcatcg ataccgatcg cgcgcagaag ttgaacaagg cgcgggccga
      421 ccgcctgagc cgcccggtag aagagatcaa caaggagatg gcggcggagg tgccgttggg
      481 ccgctacggc acggccgagg aggccggtga tttgatcgtg tttctcggat ctgaccgtgc
      541 cagctacatc accgggacga ccatcgccat cgatggcggc ctggtgcgcg cgactcagta
      601 ggaggacatg ccaatggtac acttcgttat tcagtgggat ctgccagatc agaaggccaa
      661 cctgacggtt tacgccaaca aggcgaagaa cgattggttg ccgatcaccc tgggccacaa
      721 gggcgtgatc gaagtgcgta actaccgcaa cccgctggaa gtgacgcccc atgtggcgat
      781 caccatcgat ttcgacacgc tggataattg gcgcgatttt atcgattcgg cggactatat
      841 ccggatcatg cgcgagctgc gcgtgttggg ttgccggaac ttcaccgtca acatttgggc
      901 gccgtcaaaa tattttcccg agtcgatgaa accgtaggaa gaaaactacc gttaaaatcg
      961 tccaagccgt tcaatcgctg cgctccgttc aaaacgtcga acggactcgc gctttatagt
     1021 gttagtaccg taggtccgat tagcaccgcg tacgctacac ttccaaatcg aacatctgtt
     1081 gggttaagct tcgctgaccc aacctacgaa gatgctagtt ttacccgttt tgatagggcc
     1141 agatttggtt tacccgcagg atatgaatcc catgggtctt atcgcttaga taaatgaagc
     1201 cgcggcggtc gaccaggacg tcttcggact gcgcgactgg tcggctcggc ttggtgccta
     1261 ttcttagcgt tggatcggcg gggacgaaat agcccacctc cctaggttgg cgcgcatcgc
     1321 ggatgtcgta gacccgtaag ccggcgttga agtaggtcaa gtaggccacg tcgtcgcggt
     1381 cttcgaggca ggtttggtga ttcgggtgat gatgattgtg cggtccgaag cggccggctt
     1441 tctcatagaa atttttcaat tcactctccg aaggcggctc cggcaacgga aagagcgaca
     1501 gcatgcgcgg gcgcttttca tccctgaggt cgacgatgcc ggcgagattt aaattctcgt
     1561 cgccgttctc tttaatcgcc tcatcattaa tcaacgcaag cttgcgccgc ggcagcggca
     1621 ggtaggtatg aatgccctga gtcgcggtga tgccgcggaa tgccaatcgg ctcaccaact
     1681 tcggctgtgc gagatcgctg acatcgagga taacggcgcc gccgtcgccg taggaaagat
     1741 aggcgcgctc agcttcgatg tgggccgggc cgtgcaagga aaattttaac cccatggttt
     1801 cggcgcccgt ctcctgttca ggtaaagaga agcggccgat ctcgcgtgga ttactcggat
     1861 cgcttatctc gacgctgcga taaatttttc ctgtcagtcc cgacgcgccg gcggcggcgt
     1921 ggacgaggtt gccgccatcg tagaagttgc ggtgcgtgcc ggtgctgccg gtttcccaat
     1981 ggccgatctg tttggcctcg gtgggctcgc tcacatcaaa aataacaaaa ccttcggcgc
     2041 agggctgacc ctcggcgccg ccccagccgg gggggatgcg ttcgaggccg gcgatcattt
     2101 taccgtcggc aagctgaatc tgcagggtcc aggtattttc cggcccggcg acgtaagtgc
     2161 aaaattgcgg cgcttccgga tgggtgacat cgagtatgct ccagccgcgg tgccaaagat
     2221 gggcgacgta gagataccag cggcgattct tctcctgcat ggccaactta aaggccggct
     2281 tgccgttcaa atcgtggtag cccaaggcgt cgatattttt cgcgaagtag ccttttggaa
     2341 ccgcttgcgc catggttatc gcctcacgcc gacgcccaac tgcgggcaat attttttcac
     2401 cggacagtcg ctgcacaccg gcgagatcgg ccggcagcga tgctggccga agttcaccag
     2461 caagttgttg tactcgatcc agtattgctt cggtagtttg gcgcgcagag caacttcggt
     2521 tttctccggt gtggcggttt gaatatagcc ccagcggttg gaaatccggt gcacatgggt
     2581 gtcgacgcag atgccgggct tgttgtagcc caaggtgacg accaagttgg cggtcttgcg
     2641 cccgacgcct ttgaatttga gcagctcgtc gatctcgtcg gggactttgc cgtcgtggcg
     2701 atcgatgatc gtccggcata tttcaagaat ttgccgcgcc ttggttttat aaaagcccac
     2761 cggatagatc agcttttcaa ttttcttcgc cgtcagtttg accatcgctt ccggcgtatc
     2821 ggcgatggcg aacagtcggt gcgacgcttt cgcggtggtg gcgtcctggg tacgcaaact
     2881 cagaatgcac gagatcaaca cgcgaaacgg gctttggtag gtctccgcca tcagggttac
     2941 cacgggggtc tcccacttgg gcacttcgcg gcgcaggatg cgcacggctg tatgcatttc
     3001 gcgttcggtc acgggctagt gttttgcctt tcgattggct aaattgcaac ggtatctatc
     3061 acgatcgcgc acccggcaaa agttatctat tcatggttgc tgacaagctt gtttccgctt
     3121 tgatatcgcg cacgcccgag atcatcgcgg gtgatgggct gcggccggcg gcggtgttaa
     3181 tcctgatcca agagcgcgcc gacggcgact atatcgtgct gaccaagcgc ggcgacaaca
     3241 tgccgacgca caaagggcag atcgcttttc ccggcggccg ggcgcacccg ggggacgccg
     3301 atttggtcgc caccgctttg cgcgaaagtc atgaagagat cggcgtcgtt ccggaacatg
     3361 tcagggtgct cggccggctg gacgaattca ccgccggcta cggcatggtg gtgacgccgg
     3421 tgatcggcgt aattccgcca caatacgagt ttctcatcga tctggcggag acaagcgcgg
     3481 tggcctcggt gccgatttgc tcgctgatgg agcccagcac gtatgtgaaa aatgcccacc
     3541 tctcgcccgg cggtcatccg agttaccatt tctacatcaa caatggctgg gatgtttggg
     3601 gcgtcaccgc gcgcatcctc gtgcagtttc tcgaactggc ttatgacttt gagtctgaaa
     3661 aaaaatagcc tggggctgat gctcgccggc gcgctgggac ttttttccgg ctgtgctgcc
     3721 gggccgcatt tttatgtgca gtatggggac gaataccggg agatcagtct ggaaaaactc
     3781 ctcgctgaca atccgctggc gcccggggaa aatattagag tcgccaatct cggccgttcg
     3841 gcaagcgcaa gccagcatat cgtgcaaatc cgcgaccgcg agatcttgca tgtgcataaa
     3901 ctccatgacg gcacggtgat cattctgcgc ggccacggtt atttagtgtt ggccggccaa
     3961 cgcatcgttt tgaaagccgg cgacgtcatt cacattccgc gcggtgtcgc gcatcaatat
     4021 atcaacggcg ccggtgagcc gaccgttgtc ttggcggtgt acgcgccggc cttcgacggc
     4081 aaggacaccc atccggtcgc cgtgccgtaa acaccgtaac gagtccgatc caattccgtg
     4141 aaattgacta tcatcctgtt gccgatgctc gccgcggcgt tgcttgccgc gccgtttctc
     4201 gaaccgtggt tgttcccgct cgcttgggtc gccttcgtgc cgctgttttg ggccatcgat
     4261 cgcgccgaga gttcacggcg cgctattctc tatggttggc tgatggggct gacggcgcat
     4321 ttgatcggct tccattggct cacctatacg atcagtgtct tcggtggctt gccgttcgcc
     4381 gtcagctgcg tcgtgttttt gctttatgcc gcgctacagg gaatccagct ggcgctcttt
     4441 gccctgctgg tgcgtcgtct cggagtcggc ccgttgctgc tgtttcccgc gctgttttgg
     4501 gtgtctctcg aatttctctt tccgcttttg ttcccgtggt acatcgccaa ctcccaagtc
     4561 gcgtttccct gggtgattca aaccgccgac ctggtcggtc cctacggtac tggtttcatc
     4621 gtcatatggt ttagcgcggc gctctatcgc gcgctgttca ccagcgcgga accgagcaag
     4681 agaattctgc cattggccta cgcttgtttg gcgatcgtcg ttgccctggc ttacggcttc
     4741 cagcgcttgc agagtgtcgg cgacgaaatg gccagcgcgc gcaaattatc ggtgggcacg
     4801 gtccagggaa atgtcgacat tgacatgaaa tggaatccgg tgctggcgca aaaaaatctc
     4861 gaccagcacc gccaactcac caccgagctc gatgccgtgc ccctggtggt ttggccggag
     4921 tcggcgatcg aggcgatcat tccagagacc ctgcaggcgc tgccgttgag cgtgatgccg
     4981 gcgttcaagt cggagcgcgc ctactttatc tttggcgcga aaagttttcg cggcaatccg
     5041 gggcagcccg gcggcaaagc cttcaacacc gcttttttta ccgacgccaa gggacggatc
     5101 cttgcccgct atcataaaca ggtgttactc gctttcggcg agtatctgcc cttcgccaaa
     5161 cttctttcct ggatcccggc gctacccatg gccgatggct tcacgccagg tccgggcccg
     5221 gtggtgtttc atttaccgcg ggcggtgcgg gtggcgccgt tgatttgtta cgaagatctg
     5281 ttgcccgacg tggtgcgccg cttcgtcggg cagaccggcg ccaacgttct gatcaatctc
     5341 accaatgatg cctggtatgg ccgatcggtg ggaccttggc agcatctgcg cttggcgcaa
     5401 tcgcgggcca tcgaaacccg gcgcagtttg ctgcgtgtca ctaacacggg cgtcacttcg
     5461 ttggtcaacg ccaaaggcga attggtcaag actttgccga tgtttaccgc ggcggtcatg
     5521 cacgccgaag tcgacgtctt gaatgaaacg acttactacg tacgtttcgg cgactggttt
     5581 gcttgggtga tgacgatcgc tacctttgcg ttgattctgc tgcacgcgaa gcgtagctgg
     5641 ttgagaagat aattggcttg gcgccatggt cgatgctttc agacctcagt gttggtttag
     5701 cacttcatat gcaagggaat gttaaccacg aaggacacga agcgcacgaa gttcggaaac
     5761 aatattattt cctaaacctt cgtgtcattc gtggcgaatc ttctcgtttc ggttgcggct
     5821 gtaccgcggg gcccttcgtg gtgaagccca aaattcacta accgcccgac accgccaaat
     5881 catggggacg tttgccgagg cgtttaccgc cgttggcggt gacgacgatg gtgtcgcccc
     5941 aggtgctgag cgattggccg tcggcggtga ttgccgacgg tttgaagatg tagaccatgt
     6001 tttcttgcag cgtgacgccg agttggcgcg ggctcttggc atgggcggtg atgatcgggc
     6061 cgtcgtcgcc ggcgccgcgg ccatgcatat tcaaatcgcc gcgcgctccc gccgccggac
     6121 ctgatttcgg cgcgacgctt tcggcggtcc tgatcgtcag ttcggccagt tccttcactg
     6181 tgacgccggg tttcaaatgt tccatgacgc gattgaaaat ttcgcgttgg actttgatca
     6241 gttcttgatg caccggattg accgctccga caaaaaccgg ctgcacgcct tgggcgcggt
     6301 agccgaccca gttcgcttcc agttcattga taatcaagtc gccgtgctcg agcaggcgca
     6361 tgcttgggcg ggtgagggtg cgcacgggat tcttgccgga gatccagttg caatgcaccg
     6421 gcatttccga gccgttgcgc atcatgccgt actgcgccgc ggcccaaaca tcccagtcgc
     6481 ggacaccggg tctggccgct tcaatctccg ccttgtaacc ctcttcgact atcgccatcg
     6541 aatgcgtcaa tacctggatt tcttcggcgc tcttgaggta acgcacctcg accaggatcg
     6601 gtgttgcgtc gacgagttgg gcattaggaa aggcgtcgcg gattctttgc caggtgccgt
     6661 gcagaatcgt tccttcggga gtgcgcgtac cttcgacctc ccccagtccg gtaatgccta
     6721 tacggccgtg atcgagagtt aactctttca agcgttcgac gatgacctta ccgtactcgc
     6781 gccgcgcttc gcgcacatcg gtcgtccaat cctgggtggt ggtccagcgc ggattggctg
     6841 aggtggcgat ggcggtgacg gcgccattca acggaaatac cgcggcgata tcggcgtcgc
     6901 cgccaccgcc gcagtgggtc aagtaacggg tgttagcttg aaaatccatc gaatggccgg
     6961 tgttttgcgg tgtgacgatg acgtcgatgt gctgctcggc catcttcacg cgcacggcgt
     7021 tccagcggcg gtcgcgttca gcgagggaga agcgagggta ggggtatagc tcggacatgg
     7081 aaactccgtt caaggcgttc aataagttca aatcgttcaa accgtcggac cgaactaacc
     7141 acgaaataca cgaaggtcac gaaggaattt tccgaatttc atgtggttgg tacttttcgc
     7201 ggtttattta cggtcgctgc tcagtgcggt tttcggcgcg cgcgcatgcg cgtgaccgaa
     7261 gcttggaaca atcgttccgc ttcatcggct agctctttcg acggcgcgtc agggttgata
     7321 ttttcatcgc tcatgggaat cctccgtcag gaattatcgc atctattttt acaccgttac
     7381 gaacgtcagg tcgcgcttgc cgagccgccg ggcgccgttc gcttcaatcg ccacggtgtc
     7441 gccggcgcgg atggagtcgc gcatgaagcc ccgtttgcag gcgggcttca aaataaacac
     7501 gtggccttct ttcaatagct gatctttctc ggggaaatcc ttgcgcgcgc cccagagcat
     7561 cggtgcgtct tcgcccaagc cgcggccgtg catgagggcg ccgtccggtt cgtagccggc
     7621 gccggcgact tcatcctgat aaaacttcgc gaccgcgccg aatgaaatgc cgggctttaa
     7681 gaatttgacc atgcggtcga agatgacttt agtagcgtcg aatattttct ccaattcctt
     7741 cggtttcggt ccgagggaga tgggttgcaa tgcctgggcg atgtagccgg cgtatttgcc
     7801 ttcgacttcg gtgttgatca aatcgccggc tttgagtttg cgagtggtaa aaacccgctg
     7861 cgcccagggc acttccggtc cgccggtgcc gaaaagcatc atggtgatcg gctcaccgcc
     7921 gcggcggaca atctcgcgca gcatttcggc aatcaattca tgttcgccaa ctccgacctt
     7981 ggcatgttgg atcaatactt cattggcatg gccgatgatc tcggcggcct tttcgataaa
     8041 actaacctct tcggcgcttt tcaccgaacg cacctcttgc atgacatcgg tggcattggc
     8101 gaacttgacg ttcggcagcg ccgtgcgaat tttttcaaag gttccccagg gcacgacgcc
     8161 ttccggggcg cgcaccatgc ccgagagagc gacgacgcca acacgcacgg cgcgcacttc
     8221 tttcaagcgg tcgatcaccg gttcgccgta ggaacggcgc gatggacgaa tgtcggttac
     8281 ccagtcttgc gccaggcccc accattcgat ttcgttttcg ccgcgcacca ccgctgtgac
     8341 gtcggcttcc aagggaacca cgcaaccgac ttcagtctgg gtgccgccga tctgggtgac
     8401 gtaccgtgtg tcgcaaccga actggtccca atggccttca tggggcaagg cgatgagggc
     8461 gtcgaaatcg gcctgcttca tcgcggcgcg cagcagcgac cagcgccggt cgcgttcttt
     8521 cattgagaag gttggaatga tcggtttttc aaacgccacg ggtcttcctt atgccgagaa
     8581 aaattgcacg ttgacggtcc gaccccttct ccctgtggga gaaggttggg atgagggcgc
     8641 attgcaccga tgccctcacc ctcgccctct cccacaggga gagggaatcg gaaggcttat
     8701 ttctggatct tcatgccttt atcgaagctc gagatgcccc tgccctgctt gtggcggttt
     8761 tcgatggttt tctgccattt ctcttcgtcg gagatcatcg accagtcttc ttcgccgagg
     8821 gcggtgattt gcaaatagtt ggtgtcggga tcgaagaagt aaacgctccg ttcgccggga
     8881 ggtctcagtc cgtcgctggc gcggaaatcg ccgagtgatc taccttccaa tttggggatg
     8941 cgttcgagca tcacgtcgta atcttcctgg ctgacgttga aggcgacatg gcaggcgcgg
     9001 cctttctttt tggtccgttc cgaaagttct ttgacttcct tgataccgac catttggccg
     9061 ttttgaaatt cgaatagcgt ctcgccgcgc actgtgccga ccggtttgag tcccaggcct
     9121 tcggtataga agcgcgtggc gcgggcgagg ttggcggttt caaaaatcac gtggctgatg
     9181 ccgtccttgg ccgccagcgg caggcgacgc acgcagagct cgatctggtt gtcgtcggga
     9241 tcgatgaact gaaacgtctc gacgaacggg gaatccgttg ggtggctctc gacatcgaaa
     9301 catttcacgc cgctggcgcg aattctctcg accgcggcct taaagctcga ctcatccggc
     9361 atggccaaag cgtaacgcgg cgccccgctg gccgatgccg gcacggcgac gcccggcaaa
     9421 gtcaggccaa cgatgttttc gcccgccttc acgtaggtgt ggcgcggtga gccgtcggcg
     9481 ttcttacgct gggtcaagaa cgtcagaccg acggtatcta aatagaactt ctccgaacgc
     9541 ggcagatcgc gcaccggaaa ggcgacatgg tcgagtgtct cggctcgaaa cataaacttc
     9601 tccgtttagt gccctgaaga ttttcgcttc aaaacgactt cgtcgaggat ctgcacgccg
     9661 tccttcaaca gcttggcatt ttgctccagt gtgttgagct gcacgcgcgg tgtgccgagt
     9721 aagttttgca gctccttcat gcgcggatag atcggctcga ctttgggatg ggcgacgacg
     9781 cggccttccc tggcgatcaa acgctggcct tcttccgaga tcagatagtc gtaaaaaagc
     9841 gcagcggcgt ggggatgcgg ggcattgcgc gccaaagtga ggggatagat caaagcggga
     9901 atcaaatcct cggcgaccca gtcgacgggt gcgttggtcg cgcgcagccc ttcgttgttg
     9961 taggcgtaag tcgtcaccgc gaggttgccc tcgccggcca tcatcatttg cgtcgtggta
    10021 tcgcggccga cgcggatcat gagattttgc tgggtcaact tgttcaggta attcaccgtt
    10081 cgttctttgc cccagacggc cagcatcgcc atgacttctt cgagcgacga ttcgtcaaag
    10141 agaatttcat ttttccactt gggttgcagt aagtcgtccc aactctttgg cgcgtccttg
    10201 gcgttgacct tgcgcggatt gaaggagaac actcggaagg catagttcat gttggtgtaa
    10261 aacggcgatt tcgcgtgggc gggatagatc gcccgcagcg gcgtgtcgta tttaccgatt
    10321 aagttgcgtt cgagtaaaat cggcaggagg tcggtagacg gtttgatgac gtcgaccaaa
    10381 tgtttgccgg cgttggcttc ggcaacaatg cgctgggtca cctgacgtgt ctttgagcgc
    10441 cagaagttgg cattgatgga cgggtatttt ttcttgaaac cttggatgta gcgggtcgcg
    10501 ttctcgatgc ccgagttggt gtaccacacc atctcgcctt ctcttttggc gccttcgagg
    10561 agtttcttct cgcgatcggc ggcgggcagt ttctccaagc ctcgccaaag gtcctcggat
    10621 gtctgagcgc tcgcggtaac accggcgaaa caagctgcca atatcgccgt ggcgacggcg
    10681 agtttgagcg gaaatcgcat ggcatcagcc tccttgtctc acgcttagag tctatttcac
    10741 ggcatctatg gctgcaacca atctccgcag cagatcaatt cggccggttc gtcccaggcc
    10801 ggttgatttc gtcgaggatg ctgtgatcga ttacttgtct gtaggtaagc tgcaccggct
    10861 gctccatact tttttccagc agctcaagtg atatcttgaa ctgccgcggt gaagtacgga
    10921 tagctcagcg tgaggcaatc cttaagaatg tcggcaggcg aggcgccccg caccattctc
    10981 ctaaatgccg actcgtgata accccgccgc ttcccttgtg ccgttgattt catccagcac
    11041 cattttttcc acggactgta ttttccaacc gaactcgttc accgctttgg agttgtcgaa
    11101 gttccatgct ggtacgcggg ccttttcgtc gaaggtgatt tcggcgttgg gcagatattt
    11161 tttgacgata ccggcgactt cgttgccggt ggggcttggg ccggtgccca tgtaggtttc
    11221 ttgtttcact tcctcggcga gcgccagctt ggtgtagagc tgggcgatgt cgccgacgcc
    11281 gacgatattg cgcgggccgt tggacggcca gttggcgaag ccggggttgc cgagggcggg
    11341 gtcctggatc atgcgcttcg agaacgcggt attgcctggg ccgatgacgg cggcggagtg
    11401 gactttgacg atctgaacgc cgtaggtttt gctgtagcgc tcggctaaac cgttgttgac
    11461 cagcttcatc aagccgtaaa ggcttttcgg cgcgcgcggc tcggtgtgct catcgatcgg
    11521 ttcggagccg tagccgtctt ggtagccaaa gataccgacg gtgctggcga agataatacg
    11581 ttgcattttc gccaagcgcg cggcttcgaa cagattggtc acgccgataa tgttgacacg
    11641 ggcggcgttc aaaagatccc ggcattccgg cgcgagcaat ggggtcatgt aatagcccag
    11701 ggcaaccatg cgctggatgc cgttgtccag tactaccgcc atgacttctt ccaggttggt
    11761 catgttgcct tgaacgaacg gcacgccggg aaccgaccat tggggcggcg cggcgaccag
    11821 gtcgaaggcg acgacttccg caccttgatt cgctaagttt ttcacgacat gccggccgat
    11881 gtagccgctg ctgccgacga ccatgatttt cattgaagaa cctccccaat gtgttctcgg
    11941 cttctatacg ataggcg
//
LOCUS       DASQKS010000094         6707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5092954, whole genome shotgun sequence.
ACCESSION   DASQKS010000094 DASQKS010000000
VERSION     DASQKS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5092954"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="VE170_02580"
     CDS             <1..1591
                     /locus_tag="VE170_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HYJ14344.1"
                     /translation="VRPGLLQNNPVLTNIYLIMFADLTCGALSELESGRSWNSREIAM
                     RVAACAAGLRARGLQRGDRLFILYGNNLEFFVDLLAAWSVGASVVPVDNRLTPFEVEN
                     LARTVTPKIALTDATVPMALGEALVALGVTLFGSGNLTANPATSVPSAGTLCAARLDD
                     EALILFTSGTTGVPKGVVHTHRSLRARWLSLRQSLGIDCFRRTLCLLPTHFGHGLICN
                     SLFPWLFGQDLIIAPPFSPYLLMRLGSIIDQQKITFLSSVPSMWGLSLKGSKPPQGKT
                     LQRIHCGSAPLSAHLWGQIQQWSGIRAVYNAYGITETGSWVAGTSIEEFEPEDGLVGV
                     PWGAVIKILKTADPQTPFADGAECKGAETGYVWINTPALMQGYYGQPELTDQVVSGGW
                     FVTGDMGLIDDRGRLYLKGRERDEINKGGAKVYPADVDAVVERFEPATAVCTFAIADP
                     AYGQNVAMAVVLSRQDDATLRQLHGWLKEHLAEFKIPQRWYVVDALPQNARGKVSRAT
                     VHELCATLKPVDLVGLLRRGS"
     gene            1643..1903
                     /locus_tag="VE170_02585"
     CDS             1643..1903
                     /locus_tag="VE170_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYJ14345.1"
                     /translation="MTERERSRQELIDFLRTIQRADYAPAAIDEESGLVESGLIDSLA
                     LLQIVTYLEQTYGVDFTERGIDPEDLRSVNSILDLIAREAAS"
     gene            1900..3297
                     /locus_tag="VE170_02590"
     CDS             1900..3297
                     /locus_tag="VE170_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14346.1"
                     /translation="MNPITLGTAYRNISQFHENARLIAWLSGQPWVMWLTPERRKSVL
                     FYGAIIAGAVGVFSRNAKWRDYRALTPWLEHGLAFLILLGILFALYRATVGFKQLPTI
                     VRRRPQICFHLIFWLLLALLWFFAASEGFVIAVLSVILLSFPYLIWRAGYMLLSGQRG
                     KAQNSRFRDHLFYIWPIWDGTNTPPGKGHDYLSRTMAQTPDSYARTILGGLKLLLLVA
                     LWKIVLELIGVLIYGDPKSPFSALLHDYTFGLPRVKQILSRQSAFPLIQVWFSLYLEL
                     VWETLSLAAKGHVWVGVIRLFGFNIFRNTYKPLLAQTVVEFWNRYYYYFKELMMESFF
                     LPIYARYFRNSPMLRLVAAVFAAAFVGNMYYHLLQHKEQLVDGDWHKLWSLLGARLIY
                     CALLAAGIAISMLRQQRQRSRLPAAQIGSASKLRSLRRIACVWTFFAVINFWNVVAPL
                     TIAERTRLFFTLFGL"
     gene            3323..4795
                     /locus_tag="VE170_02595"
     CDS             3323..4795
                     /locus_tag="VE170_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYJ14347.1"
                     /translation="MFQLPRTTFAVALCLVGALILTEFRLWLYWERRADVKALTRRQK
                     LKLFWIGIRLDAVIVSRGCLPVVVAALALPDALFVSSRIFFDLYLALLYFTLFFAEIA
                     GIYFFRFYDFRPNYLVFEHGVDREVLKTVVKAYPLVRIFLLSAIGAWLAVIALQFLAD
                     NTATSDHLWLADRVLTLLWLLFVGFASRGTLDHRPLNPSLASFTTHRIANEIAGCGIF
                     NLLYEWSQRAKHQFAALKSITQLPAADAAIERCRKVFARDGQFTDDGPNPLVRQITNP
                     VRATPLNVVLVVMESFTGRLTGCLGGVPALSPELDRIAQEGLLLEHCYATGERTIQGL
                     EAAVCSFPPLPGEGVVKRPQARQNFFTLANVLQALLVLELGGNDGLRGLPPETTRQNL
                     QAIIERTQKKYPQAKIILAGMKVPPNMGADYANKFGVIYPDLAKKNKALLVPFILENV
                     GGVRELNLPDGIHPTAKGHELVAANVWKVLEPVLQGLVGK"
     gene            5071..5361
                     /locus_tag="VE170_02600"
     CDS             5071..5361
                     /locus_tag="VE170_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYJ14348.1"
                     /translation="MAVRLIVTITAATGKGSELGKLYKARCEDIVNEPGCEQFEVFQS
                     VVNPDHLVILERWTDQAALDFHSKVNATRAPLPAELRVGTTQREDYTYNRTR"
     gene            5557..6414
                     /locus_tag="VE170_02605"
     CDS             5557..6414
                     /locus_tag="VE170_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ14349.1"
                     /translation="MAESLESFKTFIGKSETAADVVTASTMVKFAATLGLENPPLEKG
                     APIPPGWYGGLFPASHRPDKMRTDGQASGGGFMPAIPLPRRRIGGTRVAFHEPLRIGD
                     DVTRKTEIADLQVDDGASGAQVTIIERNSLSTSRGLCVVEERDMVMLSETRADAAPKT
                     SPAIPSEAKWNQTVEPNAPLLFRFSAIRFNSHRIHYDRDYVTKVEKLPGLVVQSSLIS
                     QLLMEMCRRELPAKRMTNFDFQNLRSVYDVDGKVTLSGKPSNDGREATLWALDGKGNL
                     AMLVAAKFA"
BASE COUNT         1367 a         1895 c         1887 g         1558 t
ORIGIN      
        1 ggttcggccc gggttgctac aaaataatcc agtattgacg aatatttatc taattatgtt
       61 tgccgatttg acatgcggtg ctctgagcga gctggagtcg ggtcgcagct ggaacagccg
      121 agaaatcgcc atgcgcgtgg cggcctgcgc ggccgggctg cgcgcccgtg gattacagcg
      181 tggcgatcgc ctgttcatac tttacggcaa caatctcgaa ttctttgtcg acctgttggc
      241 cgcgtggagc gtcggcgcca gcgttgtgcc ggtggacaat cggttaacgc cattcgaagt
      301 tgaaaactta gcacggacgg tgacgcccaa gatcgcgctc accgatgcga cggtgcccat
      361 ggctcttggt gaagcgctgg tagcgctggg ggttacgctg ttcggctccg gcaacttgac
      421 ggcgaatccg gcaacatcgg taccgtcggc cggcacactt tgcgccgcgc ggctcgacga
      481 tgaggcgttg attctgttta cctccggcac taccggagtg cccaaaggtg tggttcacac
      541 ccatcggtca ctgcgcgcgc gttggctatc tttacgccag agtctcggta tcgattgctt
      601 tcgccgcacc ctatgtttgt tgccgaccca tttcggtcat ggcttgatct gcaatagttt
      661 atttccctgg ctcttcggcc aggatttgat cattgcgccg ccattcagcc cttatttgtt
      721 aatgcgcctt gggtcgatca tcgatcagca gaagattacg tttctctcct ccgtgccatc
      781 catgtggggg ttgagcttga aaggcagtaa gccgccccag ggcaaaaccc tccagcgtat
      841 tcattgcggc tcggcgccct tatcagcgca cttgtggggc cagattcaac aatggtcggg
      901 aatccgcgcg gtgtataacg cttatggcat tactgaaacc ggcagttggg tggccggcac
      961 gagtattgaa gaattcgaac cggaagacgg cctagtcggt gtgccttggg gtgcggtgat
     1021 caagattctc aaaactgccg atccccagac gccgtttgcc gacggcgccg agtgcaaagg
     1081 cgctgaaacc ggctatgtct ggatcaatac gccggcgctg atgcaaggct attacggtca
     1141 gcccgaactc accgatcagg tggtttcggg cggctggttc gtcaccggcg acatggggct
     1201 catcgacgat cgcggcagat tgtatctcaa agggcgcgag cgcgacgaaa tcaataaagg
     1261 cggcgccaaa gtctaccccg ccgatgtcga tgctgtggtc gaacggttcg agcccgccac
     1321 tgcggtatgt acttttgcca tcgccgatcc ggcctacggt cagaatgtcg ccatggcggt
     1381 ggtgttgagc cgtcaggatg acgcgaccct gcgccagctt catggctggc tcaaggagca
     1441 tttagcggaa ttcaaaatcc cccagcgctg gtatgttgtc gacgcgctgc cgcaaaacgc
     1501 gcggggcaag gtgagccgtg cgacggtgca tgaactgtgc gcgacattga aacctgtgga
     1561 cttggtcgga ctgttacggc ggggttcgtg acgcgccagc agcgacgccg ctgttttaac
     1621 aaacaaatat tggaaattta cgatgaccga gcgggaacgc agccggcaag agctgatcga
     1681 ttttttgcgg accatccaaa gagcagatta tgcgcccgcg gccattgacg aagagtccgg
     1741 cttagttgag tctggtttga tcgattcgtt ggcgctgctc cagatcgtca cctatttgga
     1801 acagacctat ggcgtcgatt tcacggagcg cggcatcgat ccggaggatt tgcgctcggt
     1861 caattccatc cttgatttga ttgcccgtga agcggcgtca tgaatcccat caccctgggc
     1921 acggcttacc gaaatatttc gcaattccat gaaaacgccc ggttgatcgc ctggctgagc
     1981 ggccaaccct gggtgatgtg gctgacaccg gaacggcgca agtcggtgct tttttacggc
     2041 gcgataatcg ccggggccgt tggggtcttc agtcgcaacg cgaagtggcg cgattatcgc
     2101 gcactgacgc catggttgga gcatgggctg gcgtttctta tcctgctcgg aattcttttt
     2161 gcgctctacc gcgccacggt tggtttcaag cagttgccga cgatcgtgcg tcgccggcca
     2221 cagatctgtt ttcatttaat attctggctg ttgctggcgt tgctatggtt tttcgcggcg
     2281 agcgaaggat ttgtcattgc ggttctatcg gtgattttgc tgtcgtttcc ctatttgatc
     2341 tggcgcgcgg gctatatgct tttgtcgggt caacgcggca aggcgcagaa cagccgtttt
     2401 cgcgatcact tattttatat ttggccaatt tgggatggca cgaatacgcc tccaggaaag
     2461 ggtcatgatt atttgtcgcg caccatggca caaacgccgg acagctatgc gcggacgata
     2521 ctcggcgggc ttaagctgct attgctggtg gccctgtgga agatagtttt ggaactgatc
     2581 ggggtcttga tatacggcga tccgaaaagt ccgttcagcg cgctgttaca tgattacact
     2641 ttcggtttac cacgggtgaa gcaaatcctc agcagacaat cggcatttcc gctgatccag
     2701 gtatggttca gcctttatct ggagctggtc tgggagacgc tttcgctggc ggccaagggg
     2761 catgtctggg tcggcgtgat ccggctgttt ggcttcaata tttttcgcaa tacttacaag
     2821 ccgctactgg cgcagaccgt cgtagagttc tggaaccgct attattatta tttcaaagaa
     2881 ttgatgatgg aaagcttctt tctgccgatc tacgcgcgtt actttcgtaa ctcgccgatg
     2941 ttgcggctag tcgccgcggt ttttgccgcg gccttcgtcg gcaacatgta ttatcacctc
     3001 ttgcagcaca aagagcagtt ggttgacggc gactggcata aactctggtc gctactcggg
     3061 gcacgcttga tctattgcgc tttgcttgct gccggcatcg ccatctccat gctacgccaa
     3121 cagcgccaac gcagccggct gccggcagcg caaatcgggt cagcgagcaa attacgcagc
     3181 ctgcgacgca tcgcctgcgt ttggactttt ttcgccgtca tcaatttttg gaatgttgtc
     3241 gctcctctca ctattgccga acggacacga ttgtttttca cgctgtttgg tctgtaatta
     3301 gctcggagcc cgcaccagcc ttatgtttca actgccgcgt acgacgttcg ctgttgccct
     3361 ttgccttgtc ggcgcattga tcttaacgga gtttcgcctc tggctctatt gggaacggcg
     3421 cgccgacgtc aaggcgctga cccgccggca aaagctaaaa ctattttgga tcggtattcg
     3481 gttggacgct gtgatcgtca gccgaggttg cctgccggtg gtggttgcgg cgctggcgct
     3541 gcccgatgct ttatttgttt ccagtcggat tttctttgat ctttacctcg cgctacttta
     3601 tttcacgcta ttcttcgccg agatcgccgg catttacttc tttcgcttct atgatttccg
     3661 acccaactac ctggtctttg aacacggcgt cgaccgcgag gtgctcaaga ccgttgtcaa
     3721 agcctatccg ctcgtccgca ttttcttgtt aagcgcgatc ggcgcttggc tggccgtgat
     3781 tgccctgcaa tttctcgcgg acaacacagc cacaagcgat cacctgtggt tggcggatcg
     3841 cgtgctgaca ttgctctggc tgctattcgt cggtttcgcc agccgcggga ccttggacca
     3901 ccggccgctc aatccgagcc tagcttcgtt taccacccat cgaatcgcca acgagatcgc
     3961 cggttgcggg attttcaacc tgctctacga atggagtcag cgggcgaaac atcaatttgc
     4021 cgcgcttaaa agtatcaccc aactgccggc cgctgatgcg gcgatcgaac gttgccgcaa
     4081 agttttcgct cgagacggtc aattcactga cgatggcccg aatcctctgg tgcggcaaat
     4141 aaccaacccc gtcagggcga cgccgctcaa cgtggtgtta gtcgtgatgg aaagctttac
     4201 cggccggttg accggatgtc ttggcggtgt gccggcgctt tcgccggagt tggatagaat
     4261 agcgcaagaa ggtctgttgc tggaacattg ctacgccacc ggagagagga ccatccaggg
     4321 cctcgaagcc gcggtctgct cgttcccgcc gcttcccggc gaaggagtgg tcaaacggcc
     4381 gcaggcgcgg caaaattttt ttaccttagc caatgtttta caagcgctac ttgtcttaga
     4441 actcggcggc aacgacggcc tgcgcggctt gccgccggaa actacacgcc agaacctcca
     4501 agcgattatc gaacggacgc aaaaaaaata tccccaggcc aaaattatcc tcgccggcat
     4561 gaaagtgccg cccaacatgg gcgccgacta cgccaataaa ttcggggtca tctacccgga
     4621 ccttgcgaaa aagaacaaag ctctactcgt ccccttcatc cttgaaaacg tcggcggcgt
     4681 gcgtgaactg aatctgcccg acggcatcca ccccaccgcc aagggccacg aacttgtcgc
     4741 cgccaatgtc tggaaagtct tagaaccggt gctgcaaggt ttggttggca aatgattccc
     4801 gtagggcagc tcgctatctc gcgggcgccg tgctggaaat gatgacattg cgatcgaagt
     4861 gacaatcgta gcggattcag cgatgccgca aaacgacggc tatcggcctg tgcttgcgat
     4921 cttggcgtct ctgacgaaca atttgcgcgc cagacaaatt tgtgagtgtt ggcgctgcct
     4981 gcatcttgac accgtcgtcc gacctgaacg atagttcgca ctgttcttaa ccaaaccacg
     5041 cgtcaaataa tggcgcacca aggagaacct atggcagtcc gactcatcgt caccatcacc
     5101 gcggcaaccg gcaaaggcag cgaacttgga aaactttaca aagcgcgttg cgaagacatc
     5161 gtcaacgaac ccggctgtga acagtttgaa gttttccaga gcgtcgtcaa ccccgaccat
     5221 ctagtcatcc tcgaacgctg gaccgaccag gcggccctcg acttccactc caaagtaaat
     5281 gccacccgcg cgccgctgcc ggcagagctg cgcgtcggca caactcaacg cgaggactac
     5341 acttataatc ggacccgcta gaatgagttc ttgaagtttg cggacaatat aagtttgccg
     5401 gccccgaccc gatgcaaata tcgtctaccg cagcgacatc ggttccttgg cagcgttttc
     5461 gtattggttc gttctaggta tagctcattt tccttgacat gacctttgcc gccgaactac
     5521 gctccgccga gacactaacg gaaggagatg aagttcatgg ctgaatcatt ggaaagtttc
     5581 aaaaccttca tcggtaagag cgaaactgcc gccgatgtcg taaccgcatc gacgatggtg
     5641 aagttcgcgg caaccctagg actggaaaat ccgccgctag agaagggcgc gccgatcccg
     5701 ccgggatggt atggcgggtt attcccggca tcgcaccggc ccgacaagat gcgtaccgac
     5761 ggccaggcat caggaggcgg tttcatgccg gcgataccgc tgccgcgccg ccgtatcggc
     5821 ggcacgcgcg tcgcgttcca cgaaccgctg aggattggcg acgatgtcac gcgcaagacc
     5881 gagattgccg atcttcaggt cgacgatgga gccagcggcg ctcaagtcac aatcatcgaa
     5941 cgtaacagcc tgtcgacctc gcgcggctta tgcgtcgtcg aagagcgcga catggtcatg
     6001 ttgagcgaaa ctcgcgccga cgccgccccc aagacttctc cggctattcc cagcgaagcg
     6061 aaatggaacc agacggtcga acccaatgcc ccgttgctat tccgcttctc ggctattcgc
     6121 ttcaatagcc atcgcatcca ctatgaccgc gattatgtta cgaaggtgga aaagctgccg
     6181 ggcctggtcg tgcaaagctc gctgatctcg cagctgctga tggaaatgtg ccggcgcgag
     6241 ttgccggcca agcgcatgac taacttcgat tttcaaaacc tgcgctcggt ttatgatgtc
     6301 gatggcaaag ttacgctgtc cggcaaaccc agcaatgatg gccgcgaagc cacgctgtgg
     6361 gcgctggatg gcaaaggtaa tctggcaatg ttagtggcgg cgaagttcgc ctaagaaaat
     6421 aatctaaccg ccaaagcacg aaagacacga aggtttcgta ggggcgggta ttaaacccgc
     6481 cccttctttt tgccttttgc gctctttgcg gctaatttcc gaacctattc agccaatcgg
     6541 atggcgcacc aagaagtcct gcaacacttt gagcgcgcca tcgccatccc aaaacgtcat
     6601 atgcctgacg ccgggcacca acgccaattc agcgccgggg attaacggcg ccagtcgccg
     6661 cgcggtcgct gccggggtgc tgccacggcg ctcgacgccg tcgtccg
//
LOCUS       DASQKS010000095         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_474899, whole genome shotgun sequence.
ACCESSION   DASQKS010000095 DASQKS010000000
VERSION     DASQKS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474899"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..915)
                     /locus_tag="VE170_02610"
     CDS             complement(205..915)
                     /locus_tag="VE170_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ14350.1"
                     /translation="MSNAFHDQLVATRDKKHSKNHPFFELWAQGKLTKEQTAFYCTQH
                     YHFVGEFLNWLAYMASQIPERDVKNYLYENLGDEENPDDRHLDMLKDYVAASGVDRNS
                     VEGSIVLGGTEQLQNWGWMMVYRKPWPVAAAGLFIGLESQFLDICKKIVPALHQHYGY
                     KARARDIRFFEEHIYADEIHGSKGFAIVEKYCATPELQAAAIKQVEDATIRRWRYMNN
                     IYWYALHGKEDDTPKLPS"
     gene            complement(1044..>2757)
                     /locus_tag="VE170_02615"
     CDS             complement(1044..>2757)
                     /locus_tag="VE170_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14351.1"
                     /translation="GAQSMLRVLGKMGVEKIFASPGSEWSPLWEALAEPGVEGMPQYY
                     STRHEEIAVGMASGYAKSTGKLPAVVIHTTVGALHATMAMRAALHEQVPMVVFAGESI
                     GFGEDEGPDVGGQWQGHLGDMGGPAKLVDHTVKWSFGVNTKSIVPSTVQRACRLAMAG
                     PRGPVFVSMPMEYMFDTMTKDVPSDLGSSPLATADPQGIEDLANLLAGAQHPVIVTED
                     AGKTLKSVNLLVEIAELLGCPVVETRSTGVINIPRTHPLHSGYDAKEGVQGADVIFLL
                     AVIAPWHPASITPSKGAKVAMLDENPLRIEIPYYGYQSDLCLTGEIESSLEHLLAALK
                     KKVSKGDSARTRRADELKATNDARRKKWRDEAIALGSQKPMDTRWVAHEISQVLPKDA
                     MVVEETITHRLAVHRYLDNLTPGSFFNGCIGGLGTGLATALGVKAANPKRPVICLIGD
                     GSFNYDPAPAAFGAAQEHNLPFLTIMFNNQGYLSQKSGIPKYYPGGWAVKSGNFSGLH
                     ITPCPEYSQMIKAWDGYGEKVEEPGEVRKAVERGLRAVAGGQSALLDIRLKTVESIVE
                     RGEG"
BASE COUNT          563 a          833 c          771 g          590 t
ORIGIN      
        1 cgaaccatta ggaataattt cgtggccggt gccggaccag ccgacgacga ccccgccatc
       61 tatgcgaata ctcatgctaa cttccttccc gtgatttcct actgctaggg gtttgggaca
      121 aaaccgtggt aagagcctgg cgatcggccg gccctccgac aacaaacctc gcgtttctag
      181 tggacgaatg aaataataaa ccgcttacga cggcagcttc ggcgtgtcgt cttccttgcc
      241 gtgcagcgcg taccagtaaa tattattcat ataacgccag cgccggatgg tggcgtcctc
      301 gacctgcttg atcgccgcgg cttgcagctc tggggtggcg caatactttt cgacgatggc
      361 gaagcctttc gaaccatgga tctcgtcggc gtagatatgc tcttcgaaaa agcggatgtc
      421 ccgggcgcga gccttataac cgtagtgttg gtgcaaggcg ggaacgatct tcttgcaaat
      481 atccaagaac tgcgattcca gaccgatgaa cagcccggcg gcggcgaccg gccagggctt
      541 ccggtagacc atcatccaac cccagttttg caattgctcg gtgccgccga gaacaatcga
      601 gccctcgaca ctgttacgat cgacgccgct cgccgcgacg tagtctttca acatgtcgag
      661 gtggcgatca tcgggatttt cctcgtcgcc aagattttca tacaagtagt tcttcacatc
      721 gcgctcggga atctgcgacg ccatataggc cagccaattc aaaaattcac cgacgaaatg
      781 ataatgctgg gtacagtaga aagccgtctg ctccttggtc agtttgccct gcgcccagag
      841 ttcgaaaaac ggatggttct tgctatgctt tttgtcccgg gtcgcgacta attgatcgtg
      901 aaaagcattg ctcataactc acctcccgca aatatggatc aataacgctg cgccggtaat
      961 ttgcccttgg taaaaataac gacgccggcc gaatatcggc atacgatacc cggccggcgt
     1021 cgacccgtta aaaccgctac gagctagcct tcgccgcgct cgacgattga ctccaccgtt
     1081 ttgagccgaa tatccaacag cgctgactgg ccgccggcga ccgcgcgcag gccgcgctcg
     1141 acggctttgc ggacttcgcc cggttcttcg actttctcgc cgtaaccatc ccaggctttt
     1201 atcatttgcg aatattccgg gcagggggtt atgtgcaggc ccgaaaaatt tcccgatttg
     1261 accgcccaac ctccaggata gtatttagga atacctgatt tctgggatag atagccttga
     1321 ttattaaaca taattgtcag aaacggcagg ttgtgctcct gggccgcgcc gaaggccgcc
     1381 ggcgccggat cgtagttaaa cgaaccgtcg ccgatcaagc agataaccgg acgcttggga
     1441 ttggccgctt tgacgccgag ggcggtcgcc aaaccggtgc cgaggccgcc gatgcaaccg
     1501 ttgaagaacg atcctggcgt taggttgtct agataacgat gcaccgccaa gcggtgggtg
     1561 atggtctctt cgacaaccat cgcgtccttg ggcaatacct ggctgatctc atgggcgacc
     1621 caacgggtat ccatcggttt ttgactgccc agagcgatcg cctcatcgcg ccatttttta
     1681 cgccgggcgt cgttggtggc cttgagttca tcagcgcggc gcgtgcgcgc cgaatcgcct
     1741 ttggaaactt ttttcttcaa ggcggcgagc aaatgctcca gcgacgactc gatctcgccg
     1801 gttaggcaga gatccgattg gtagccatag tagggaatct cgatgcgcag cggattctca
     1861 tcgagcatgg caaccttggc gcctttcgaa ggcgtgatcg acgccggatg ccagggggcg
     1921 atcactgcca ggagaaaaat cacatcggcg ccctgcacgc cttctttggc gtcatagccg
     1981 ctatgcagcg gatgggtgcg cggaatgttg atcaccccgg tgctgcgggt ttccaccacc
     2041 ggacagccga gcagctcggc gatctcgacc aacagattga cgctcttcaa agtcttgccg
     2101 gcgtcttcgg taacgatcac cggatgctgc gcgccggcga gcaaattggc aagatcttcg
     2161 atgccctggg gatcggcggt ggccagcggc gaactgccga gatccgacgg cacatccttg
     2221 gtcatggtgt cgaacatata ttccatcggc atcgatacga acaccggccc gcgcggtccg
     2281 gccatggcca gacggcaggc gcgctgcacg gtggacggaa cgatcgactt ggtattgacg
     2341 ccgaagctcc atttgaccgt gtgatcgacc agcttagccg gtccgcccat gtcgcccaga
     2401 tgtccctgcc actgaccgcc gacatccggt ccttcgtctt cgccgaagcc gatggattcg
     2461 ccggcgaaca ccaccatggg cacctgctcg tgcagcgcgg cgcgcatcgc catggtggcg
     2521 tgcagcgcgc ccaccgtggt gtgaatcacc accgcgggca gcttgcccgt ggacttggca
     2581 taaccgctcg ccatgccgac ggcgatctct tcgtggcgcg tgctgtagta ctgcggcatg
     2641 ccttcgacgc ccggctccgc cagtgcttcc cataacggcg accattccga tcccggcgac
     2701 gcgaagattt tttcaacgcc catctttccc aggacacgca acatcgactg cgcgccg
//
LOCUS       DASQKS010000096         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_903380, whole genome shotgun sequence.
ACCESSION   DASQKS010000096 DASQKS010000000
VERSION     DASQKS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903380"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..1540
                     /locus_tag="VE170_02620"
     CDS             533..1540
                     /locus_tag="VE170_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ14352.1"
                     /translation="MIWFIACGGVANEPIAGRPAHHMAGGFRNPNADFQRPSTWTRWR
                     FVVRRLWTTSFSPRGFVAPRVANDGAVLRAGSVNPSITWIGHATLLVQINGVNVLTDP
                     QWSERASPVSWGGPRRLNPPGLAFEDLPRIDAVLISHDHYDHLDLNTVKRLAAVHDPL
                     FLVPLGLKAWFAENGMTRVEEMDWWQEREFRAVKFVCVPAQHFSQRTLSDANQRLWAS
                     WAMVSPERRLYFSGDTGYFAGFKEAGQRLGPFDIAAIAIGAYQPPEIMKAVHLTPEEA
                     VQAFIDLKARLLLGIHWGTFDLAEEPIEEPPQRMLADFHRRNIDASRAWIFKLGETRP
                     W"
     gene            1665..>2098
                     /locus_tag="VE170_02625"
     CDS             1665..>2098
                     /locus_tag="VE170_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HYJ14353.1"
                     /translation="MKRSGTADLPLHGGRVPLWLAERMTKLGTAITEAIIYDYGSSAF
                     LSRLSDPFWFQALGAVMGMDWHSSGITTSVMGALKRGLATRADELGVYICGGRGRFSR
                     NTPQELRSIADRRGFDGEALVRASRLTARVDNNAIADGFQIYL"
BASE COUNT          416 a          634 c          596 g          452 t
ORIGIN      
        1 cttgtcgcgc agcgagcgta accccgaggt cataataaaa agctgctgcg cacgatggct
       61 taccagcgcg agtttaccct tgctgagccg ctcgccgccc ttgacgatct gggtatcggc
      121 cagagctact aacccctgct ccactttaat ccctacgcaa aatgtcatgg ttgccaatcg
      181 aagagtgccg atcagtcgcg aagcagatgc ctttcactac cggcaagctt atttgataaa
      241 tctaatattg catatttttt tcgattctaa agccccgcac ttttttaagt gaccaggctc
      301 cggcggcggt gcaccagcct ttttcgcaat acaagacgcg acccccatca gactttccga
      361 tcttggcaga gtttcaaacc cgggccttca gcgccgatct atcgggaagc ttgcgaaata
      421 aacgagccaa gtcagcgtca gggtttctaa atatttcgac tagcgtttcg cccactagaa
      481 atgtttcaat agcggctgct ctatgcgccg cggttactct gcactttttt ttgtgatctg
      541 gttcatcgcc tgtgggggtg tcgcgaacga accgatcgcc gggcgaccgg cgcaccacat
      601 ggcgggcggt tttcgtaatc ccaatgcgga tttccagcgc ccgtcgacct ggacgcgctg
      661 gcgcttcgtg gtgcgccgat tgtggacgac ttccttctca ccgcgggggt tcgttgcgcc
      721 gcgcgtcgcc aacgacggcg ccgtcctgcg cgccggtagt gtcaatccga gcattacctg
      781 gatcggtcac gcgaccctgt tggtacaaat caacggcgta aatgtcctca ccgacccgca
      841 gtggagcgaa cgagcgagtc cggtgtcctg gggcggcccg cgccggctga atcctcccgg
      901 gctcgcattc gaagatttgc ctcggatcga tgcggtgttg atctctcacg accactacga
      961 ccatctcgac ttaaacaccg tgaagcggct ggccgcggtg cacgatccgc tctttttggt
     1021 tccactcggt ctcaaggcat ggtttgccga gaacggaatg acccgggtcg aagagatgga
     1081 ctggtggcag gaacgcgagt ttcgcgccgt gaaatttgtc tgcgtgccgg ctcagcattt
     1141 ttcccaacgc acattgtccg atgcgaacca acgactgtgg gcgtcatggg ccatggtgag
     1201 ccccgagcgg cggctttatt tctcgggtga taccggttat ttcgcgggat tcaaagaagc
     1261 cggccagcgg ctcggtccgt tcgacatcgc ggcgatcgcc atcggcgcct atcaacctcc
     1321 agaaatcatg aaggccgtgc atctgacacc ggaagaagcg gtgcaggcct tcatcgacct
     1381 caaagcccgc ctgctgctcg gcatccactg gggcaccttc gatctcgcgg aagagccgat
     1441 cgaggaacca ccgcaacgga tgctagccga tttccaccgc cgcaacatcg atgcgtcacg
     1501 cgcctggatc ttcaagctcg gcgaaactcg tccgtggtaa aatcagttcg aagagtcaac
     1561 caacagcgcg gtaatcaggc tgtgaaaaaa agctcgcgtc cgctgtgctt tgccgattcg
     1621 ctcgattgat ttggccgccc aacgggatac cgtagaaaca ggatatgaaa cgctccggaa
     1681 ccgcagatct gccgctgcac ggcggccgcg tgccgctgtg gctcgctgaa cgcatgacta
     1741 aactcggcac tgcgatcacc gaggcgatca tttatgatta cggttcctcg gctttcctgt
     1801 cgcgactgag cgatcccttt tggttccaag cgctgggcgc ggtgatgggc atggactggc
     1861 actcttcggg catcacgaca tccgtcatgg gcgcgctgaa gcgcggtctg gcaacgcgcg
     1921 cagacgagct cggcgtctac atatgcggcg gccgcggcag attttcgcgc aacacgccgc
     1981 aggaattgcg ctcgattgcc gaccgtcgcg gattcgacgg cgaagcattg gtgcgcgcga
     2041 gccggctaac cgcgcgggtc gacaacaatg ccattgccga cggctttcag atctacct
//
LOCUS       DASQKS010000097         6324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5945879, whole genome shotgun sequence.
ACCESSION   DASQKS010000097 DASQKS010000000
VERSION     DASQKS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5945879"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VE170_02630"
     CDS             <1..727
                     /locus_tag="VE170_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYJ14354.1"
                     /translation="VISRNIDVGQTVAASLSAPTLFTIANDLTKMEVHTNVDEADVGN
                     ITDGQEVSFTVDAHAQRRFRGKVNQVRNAPINVQGVVTYNSVVRINNKELLLKPGMTA
                     NVQFLVAQKEDALAIPNVALRFRPPEEKNEAQELLRQEQGRVGGRIAARRTSRGGGSG
                     VVGGEGRRVRQVKVYVFKDGKALAVDVQVGITDGSKTEIIAGLNENDPVIIGMSTAAT
                     APPQGGVASPFQPQPVPGGFRGR"
     gene            724..1437
                     /locus_tag="VE170_02635"
     CDS             724..1437
                     /locus_tag="VE170_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14355.1"
                     /translation="MNEVIRVQDLQKIYRMGDVEVPALRGINLTIERGEFVAVMGSSG
                     SGKSTFMNIIGCLDRPTGGNYWLEGEAVAALSADDWAHIRNKRIGFVFQGFNLLSRTT
                     ALENVELPMMYNGFAGKERHRRAVEVLDLVGLGERHDHTPNRLSGGQQQRVAIARALV
                     NKPSLILADEPTGNLDSATSSEIMGLFQHLNSDQGITIVLVTHEADIAEHAKRQIIFR
                     DGAVISDKPNNQVLVKLEA"
     gene            1450..2655
                     /locus_tag="VE170_02640"
     CDS             1450..2655
                     /locus_tag="VE170_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14356.1"
                     /translation="MTFRIALRALARNKLRAFLTMLGIIIGVSAVIAMVAIGEGAKST
                     IRSQISALGTNVLIVLPGSNVQGGVRAGTGNVNTLVDTDARAMTRELRSVAYASPVMR
                     RQEQLIAGNLNWGTLAQGVAPEFEQIRDWQIEAGRFLHEGDMDSAAKVAVIGQTVARQ
                     LFGADDPIDSVIRIRNIPFRIVGVLAGKGQTGQGQDQDDTVMIPYTTMQKRLMRITFV
                     QSIVVRAVSAEKVQEAQEQITLLLRQRHRIAEGREDDFTVRNLSDIAEAAQSTARVMA
                     ILLGSVAGISLLVGGIGIMNIMLVSVTERTREIGIRMAVGARSRDIMLQFLVEAVVMA
                     ATGGIIGIFLGIGSSELLKTWAQWPTLIDPIVVAVAFLFSGAVGVFFGFYPAKKAAGL
                     DPIDALRYE"
     gene            2978..4228
                     /locus_tag="VE170_02645"
     CDS             2978..4228
                     /locus_tag="VE170_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14357.1"
                     /translation="MRNLSASLVLIGALLLAASAPAQVIVLSRATVIDGSGAPPQKDI
                     TIVMENGRIRDMGPASKTPAPAGATVLDLTGKFIVPGIINAHGHVGAKTEPQLRQYAL
                     YGVTTATSMQTDPDEVVQVREAQKKGELRGARVSTVKYRFAPDPEVATPEQARAKVDQ
                     IVAAGADYIKVWVDSGFGTRAKLSPEFCSAVLEQARKHGKLTLAHAYELADARMLVER
                     GVNILAHNVRDREVDGDFIALLKQRNVTLNPTLIRDEFLFAYGDAPAWIDDPFFLKFV
                     PPERLAVLKTKIRDEQAKHPQRALFKAGFEMNKINLKKLSDAGVRIALGTDSGGSADR
                     FFIQGFSEHREMELMVQSGLTPMQVIQSFSKGASESLGIAKDFGTLAKGKAADLLVLE
                     RNPLDDIANMRSMHTIYLGGKKFE"
     gene            4513..5157
                     /locus_tag="VE170_02650"
     CDS             4513..5157
                     /locus_tag="VE170_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14358.1"
                     /translation="MNIELTLISIIQGVAFSFLADNSSRVLVNRQFIFWPYAITGLLI
                     ILLFWSRSLIHTLTVIRWPLKFGHNFMYVACTLVETIAFTQLTNPLHWYALNSVFALL
                     AGILFALDLRLIRDRIADSAGQIGCRLYAIVEREQLLNIRLVIPTTLIFNLLAMLAAY
                     YWALPFVAKGGHVIVGMVQLGATIGYLVYVTRFFTRIIPLIVQTRQEWRDDVRA"
     gene            5365..>6324
                     /locus_tag="VE170_02655"
     CDS             5365..>6324
                     /locus_tag="VE170_02655"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14359.1"
                     /translation="MVRQNVWLCGFGSAHAVSCCRSSRRISTQQRERLTRLRGVKMEP
                     VGFVGLGTIGGVVARNILQAGYAMVVYDIAPEAARKLTEGGARFAESAAAVARDCRVV
                     FTSLPGPREVEAVALGEHGLVHGVRDRSIYVDLSSSRADLIRRIGAEFATRGGWVMDA
                     PLIVGRKGIANRSVQVLASGSAEAYGEVKPLFDAFSDTVVYTGDLGTGTVIKLAHNLV
                     RRGIGLAIGEGLVLGAKAGVDPELLWDCMHWGLDVQLNQLKNHFSDTVFTGNYEHPAS
                     FGIGLSRKDVGLATDLGRQQNVPMPIAALVEQFMIHAISRGWSE"
BASE COUNT         1326 a         1792 c         1877 g         1329 t
ORIGIN      
        1 tgtgatttcg cgcaacatcg atgtcggcca gactgtcgcg gcatcgctgt cggcgccgac
       61 cctgtttacc atcgctaacg atctaaccaa gatggaagtg catacgaatg tcgacgaggc
      121 ggatgtcggc aatattactg acggtcaaga ggtgagcttt accgtcgatg ctcatgcgca
      181 gcggcgcttc cgcggcaagg tgaatcaagt gcgcaacgcg ccgatcaatg tccagggcgt
      241 ggtgacctat aattcggtgg tgcgcattaa caacaaagag ctcttgctca agcccggcat
      301 gaccgccaac gtgcagtttc tcgtcgcgca aaaagaagac gcgctggcga ttcccaatgt
      361 cgcgttgcgg ttccgcccgc cggaggagaa aaacgaagcc caagagttgc tgcgccagga
      421 acagggacgg gtgggcggac ggattgccgc gcggcgcacc agccgtggcg gcggcagcgg
      481 tgtcgtcggc ggcgagggcc ggcgcgtgcg ccaagtcaaa gtttatgtgt tcaaagatgg
      541 gaaggccctg gcggtcgacg tccaggtggg catcaccgac ggctccaaaa ccgagattat
      601 cgccggcctc aatgagaatg atccggtgat catcggcatg tcgactgccg ccaccgcgcc
      661 cccccagggc ggtgtggcaa gcccatttca accccaaccc gtcccaggcg gatttagagg
      721 acgatgaacg aggtcattcg cgtccaagat ttgcaaaaga tctaccgcat gggcgatgtc
      781 gaagtgccgg ccctgcgcgg cattaaccta accatcgagc gcggcgaatt cgtcgcggtg
      841 atgggctcct cgggttccgg caagtcgacg ttcatgaata tcatcggctg cctcgaccgt
      901 ccaaccggcg gcaactactg gctcgaaggg gaagcagtcg cggcgctcag cgccgacgac
      961 tgggcgcata tccgcaacaa gcgcatcggc ttcgtatttc aaggatttaa cttgctgtcg
     1021 cgcaccacgg cactggagaa tgtcgagctg ccgatgatgt acaacggctt cgccggcaaa
     1081 gagcgccatc gccgcgcggt ggaagtttta gatctcgtcg gtttgggcga gcgtcacgac
     1141 cacactccca atcgtctttc cggcggccag cagcagcggg tcgccatcgc ccgcgcgttg
     1201 gttaacaagc cgtcattgat tctcgccgac gagcccaccg gtaatctcga ttccgccact
     1261 agcagcgaga tcatgggctt gtttcagcac ttgaatagcg atcaaggtat taccattgtt
     1321 ctcgtcaccc acgaggcgga catcgccgag catgccaagc ggcaaatcat ttttcgcgac
     1381 ggcgcagtga tttccgacaa gcccaataac caagtgttgg tgaaactgga ggcgtgaatg
     1441 cgctggttga tgacatttcg gatcgcgctg cgcgccctgg cgcgcaacaa gctgcgtgcg
     1501 ttcctgacca tgctcggcat catcatcggt gtcagcgcgg tgatcgccat ggtcgccatc
     1561 ggtgagggtg cgaaatcaac aattcgctcg cagatctctg ctttgggaac caatgtcttg
     1621 atcgttttgc ctggcagcaa cgtccaaggc ggcgtgcgcg ccggcaccgg caacgtcaac
     1681 acgctggtcg ataccgacgc ccgcgccatg acccgcgaac tgcgatcggt cgcctacgct
     1741 tcgccggtca tgcgccgcca ggaacaactg atcgccggca acttgaactg gggaacttta
     1801 gcccaaggcg tggcgcctga gtttgagcaa atccgcgact ggcagatcga ggccggacgc
     1861 tttctgcacg agggcgatat ggactccgcc gccaaggtcg cggtcatcgg ccagaccgtg
     1921 gcgcgccagt tgttcggcgc cgatgacccc atcgattccg tgatccgcat tcgtaacatt
     1981 ccatttcgca tcgtcggcgt tctcgccggc aaaggccaga ccggccaagg ccaggatcaa
     2041 gacgataccg tgatgatccc ttacacaact atgcagaaac ggctcatgcg catcaccttt
     2101 gtgcagagca tcgtcgtccg tgccgtcagc gccgaaaagg tccaggaagc ccaggagcag
     2161 atcacattgc tgttgcgcca gcgtcatcgc atcgccgaag ggcgcgagga cgatttcaca
     2221 gtgcgtaacc tttccgacat cgccgaagcg gcacagagca cggcgcgggt gatggcaatt
     2281 ctcttgggca gcgtcgccgg gatatcgctg ctagtcggcg gcatcggcat catgaacatc
     2341 atgctggtgt cggttaccga acgcacccgc gagatcggca tccgcatggc cgtcggcgcg
     2401 cgcagtagag acatcatgct gcaattcctc gtcgaggccg tcgtcatggc cgccaccggc
     2461 ggcatcattg gcatcttcct tggcattggc agttccgaac tcctcaagac ctgggcccag
     2521 tggccgaccc tgatcgatcc aattgtggtt gccgttgcct ttctcttctc cggcgccgtc
     2581 ggcgtcttct tcggttttta tcccgccaag aaagcggcgg gtttggatcc tatcgacgcg
     2641 ctgagatacg agtagcggcg caaagccctg gtttgtttct tacccgggag ccgatgagat
     2701 ggccaagcgt ggcaatgtca aggtcaaagg acccgacggc gtgacgatcg acctgtccga
     2761 catggttggc gcggcaccaa ataattagcc atcgcgcgca gggcttccac gcgcgtccag
     2821 atttttgcgg cctccatcgg cgcagcaaac ctaagcaaca cattcatagt gcaagcgctt
     2881 aaatatgcgc cgatacgcga tgcgagttag gggaatctca atggcaagca gctatcgatg
     2941 tcgaaatgag atgctggcta agtaaggagt aggacctatg agaaatctaa gcgcttcgct
     3001 tgttctaatt ggcgcactgc tgctcgcggc cagcgcgccg gcgcaagtta tcgtgctcag
     3061 ccgtgccacg gtgattgacg gcagcggcgc gccgccgcag aaggacatca ccatcgtgat
     3121 ggagaatggc cgcatccgcg atatggggcc agcgtctaag actccagccc cagccggcgc
     3181 caccgtgctc gatctcaccg gcaagttcat cgtgcctggc atcatcaatg cccatggcca
     3241 cgtcggtgcg aaaaccgagc cgcagttgcg ccagtacgca ttgtatggcg tcaccaccgc
     3301 gacgagtatg cagaccgatc cagacgaagt cgttcaggtg cgtgaagcac agaagaaggg
     3361 cgagttgcgc ggcgcaaggg tttccaccgt gaagtaccgc ttcgcgcctg atcccgaagt
     3421 cgcgacaccc gagcaggcgc gcgctaaggt cgaccagatc gtcgccgcgg gcgccgatta
     3481 catcaaagtg tgggtggata gtggattcgg cacccgggcc aagctcagcc ccgagttttg
     3541 cagcgccgtc ctcgaacagg cgcgcaagca tggcaaactc acattggcac atgcgtatga
     3601 actcgccgac gcgaggatgc tcgtggagcg cggcgtgaat attctagccc acaatgtccg
     3661 cgaccgtgag gtggatggcg acttcatcgc gctgctcaag cagcgcaatg tgacgctcaa
     3721 tccgaccttg atacgcgacg agttcctgtt cgcctacggc gacgccccgg cctggatcga
     3781 cgatcctttc ttcctcaagt tcgtgccgcc ggagcgcctg gcggtgctga aaaccaagat
     3841 acgcgacgag caggcgaaac acccgcagcg cgccttgttc aaggcgggct tcgagatgaa
     3901 caagatcaat ctaaagaagc tctcggacgc cggggtgcgc atcgccttag gcaccgatag
     3961 cggcgggtcc gccgaccgct ttttcatcca gggtttctcc gagcaccggg agatggagct
     4021 gatggtgcag tcgggcctga caccgatgca ggtgatccag tccttctcga aaggcgcctc
     4081 ggagagtctg ggcatcgcca aagacttcgg cacgctagcc aaaggtaaag ccgccgacct
     4141 acttgtgctg gagagaaatc cgctcgacga tatcgccaac atgcgctcca tgcacaccat
     4201 ttatcttggc ggcaaaaagt tcgagtaaaa gacattgaga gacgatgagc gacgggcgcc
     4261 cagctgataa cgacgcggat tggcggtttt tcaagtttcg cgtgagccgt tatgacgata
     4321 atcgatcgat gggtcaggag atccatagag agaccgagca aaatcagtgc aagcattagt
     4381 cgggtgaaat aaaccgatag aaaaatgatt gaatgatgcg tccggtgtgg atcgttgaga
     4441 acaatggccg atcaaatttc caccaataaa gaacgatctt ctcccagcca aacacagttg
     4501 gacgggctag tggtgaacat cgagctgacg ttgatcagca tcattcaggg tgttgcgttt
     4561 tcttttctcg ccgataattc cagccgtgtg ctggttaata ggcaatttat attctggccc
     4621 tatgccatca ccggactgct gatcattcta cttttttggt cccgatcact gatccacacc
     4681 ctgactgtga tccgctggcc gctaaagttc ggtcacaact tcatgtacgt cgcttgtact
     4741 ctagtcgaga caattgcgtt cacacagctg actaatccgt tacactggta cgccttgaac
     4801 tcagtgttcg cgctcctggc cgggatacta tttgctttag atttgcgttt gatccgcgat
     4861 cgcatcgctg atagcgccgg ccagataggc tgcagactgt acgccatagt cgagcgggaa
     4921 cagctgctaa atatacggtt ggtcatcccg acaaccttga ttttcaatct tctggcaatg
     4981 ctggcggcgt actattgggc gttacccttt gtcgcaaaag gcggtcatgt cattgtcggc
     5041 atggtgcagc tcggggcaac catcggttat ctggtttacg tcacccgctt ttttacccgg
     5101 atcattccgc tgatcgtaca aacccgccaa gagtggcgcg atgatgtacg ggcctgaacg
     5161 acgcactgtc ataagggagc ggtttgagcc ggcgaatttc gttgccgttt acgaaagcgc
     5221 gctggcttcg tctgtattta ttcaggatag ctgtgcgcca ccttctggct atcgatgtac
     5281 cgtccacatg agaatcttga tttggagaac tgcctgaatt gttgttgacg attccacgcg
     5341 ccggcgatat cgcttcttgt ggacatggtc cgacaaaatg tttggctgtg cggtttcggt
     5401 tctgctcacg cggtcagctg ttgtcgttca tccaggcgaa ttagcactca gcaacgtgaa
     5461 aggttaaccc gactacgagg tgtcaaaatg gaaccagtgg gttttgttgg gcttggaact
     5521 attggcggag tggttgccag aaatattctg caggccggct atgcgatggt ggtttatgat
     5581 atcgcgccgg aagcagcgcg gaagctaaca gagggtggtg cgcggttcgc tgaatcggct
     5641 gcggctgttg cgcgggattg ccgggtggtt tttacctcac tgccggggcc gcgagaggtt
     5701 gaggcggttg cgcttggcga gcacgggctg gttcacggag ttcgcgaccg cagcatctat
     5761 gtcgaccttt ccagcagccg tgccgacctc atccggcgta tcggcgccga gtttgccacc
     5821 cgcggcggtt gggtgatgga tgcgccgctg atcgttgggc gcaaaggcat tgccaatcgc
     5881 agcgttcagg tattggcgtc gggcagcgcc gaagcctacg gcgaagtgaa gccacttttc
     5941 gacgccttca gcgacacggt tgtttacact ggcgacttgg gcaccggtac ggttattaaa
     6001 ctagcgcaca acttggtgcg ccgcggcatc ggcttggcga tcggcgaagg cctcgttttg
     6061 ggtgcaaagg ccggggtgga cccggagctg ctctgggact gcatgcattg gggcctagat
     6121 gtccagctca atcaacttaa aaatcatttc tccgatacgg tgtttacagg aaactatgaa
     6181 catccggcga gtttcggcat tggactgtcg cgaaaggacg tcggcctagc caccgatttg
     6241 ggacgtcagc aaaatgttcc catgcccatc gcggcactag tcgagcaatt tatgattcac
     6301 gccatcagcc gcggctggtc ggag
//
LOCUS       DASQKS010000098         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4674737, whole genome shotgun sequence.
ACCESSION   DASQKS010000098 DASQKS010000000
VERSION     DASQKS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4674737"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2303
                     /locus_tag="VE170_02660"
     CDS             <1..2303
                     /locus_tag="VE170_02660"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ14360.1"
                     /translation="DKETIVMAKAEFKVVGTSPARMGGIDRVIGKGVYGIDLMLKDQL
                     QGAILRSQYAHAKIISIDTSEAKKIPGVHAVVTAEDAPNVRYGRSYIDRHILARGKVH
                     FMGDPVAAVAADTLAIAKQALKKIKVAYEPLPVALDAEEATKPDAPVVHEDMPLPKTT
                     PAGMKNVCGHAVVHVGDPDKAMAEADLVVEEVYETKMIHPQYLEPRIAAAQMEPNGRL
                     TVWANAQAPFPVRQETAKMLGLRLNDVRVISAGLGGGFGGKGSGITSGAAVEPICGLL
                     AIKAKRAVMIVLDKAEETISTTIRSGARMYIKTGVKKDGTILARTGKVFYDAGGYSGF
                     GVQAGGRCTNMLGGWYLMPNCHIEGFTVYTNKQVCGPVRGPGGPQAAFAVESHMDSIA
                     AKLGMDPTEFRLKNTPKAGDKIVGVAKLRDVSLGETIKIAKEKIGWGTVKLEKNQGIG
                     LATGSWIESAGPGGGSIVKVNEDGSVTVHIGKIDMGTVPGFGIPMIVAEELGIPVSDV
                     TVVNVDTDASPWDAGTVGSRGILVSGGASRLAAIDARTQLFKMAAGKLEASPDDLEIV
                     DKQIRVKGTPAKTIALATVATDAHNVIGEVIGRGYCDNLALVAEEKAHGSSQPFTTHA
                     CIVEVDTMTGNVKILKYVAVHDIGQPIHLAAVEGQIEGATAMSIGQALCEQVVFDKEG
                     KTLNPSFVDYLMPTINMMPRIETTLVHGYPGAGPYGAKGAGEIACVPPMACIANAIYN
                     ATGVRVTKLPLSPENVLRALKEAGKA"
     gene            2414..2677
                     /locus_tag="VE170_02665"
     CDS             2414..2677
                     /locus_tag="VE170_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14361.1"
                     /translation="MKTTFAITALALELVTSPVDAQNVKITPVGSHPGELCANDRATI
                     FEDLTGVRILYDVAYNLTGTDDPRLGEIHLILLSHLHGDHMGD"
     gene            complement(2674..2937)
                     /locus_tag="VE170_02670"
     CDS             complement(2674..2937)
                     /locus_tag="VE170_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14362.1"
                     /translation="MKTPPRSVLERHTSATGTLIAPSTCGQKVIGLPPRIFWILAAIK
                     ALMFRVITSAAFFAAATSAVMEFGPAGTLSEFAHVPAPSAFSF"
BASE COUNT          712 a          951 c          910 g          527 t
ORIGIN      
        1 ccgataagga gaccatcgtc atggcaaaag ctgaattcaa agtcgtcggc accagcccgg
       61 ccagaatggg cggcatcgac cgcgtcatcg gcaaaggcgt ctatggcatc gacctgatgc
      121 tcaaagacca gctgcagggc gcgatcctgc gcagccaata cgcccacgcg aaaattatca
      181 gcatcgacac cagcgaagcc aagaaaatcc ccggcgttca tgccgtcgtc accgccgagg
      241 atgcgccgaa tgtgcgctat ggccgcagct atatcgaccg ccacatcctg gcgcgcggca
      301 aagtgcattt catgggcgac ccggtggccg ccgtcgccgc cgatactctg gccattgcca
      361 agcaggcgct caaaaaaatc aaagtcgcct acgagccgct gccggtggcg ctcgacgccg
      421 aggaagcgac caaaccagac gcaccggtgg tgcacgaaga tatgccgctg ccgaaaacca
      481 ctccggctgg gatgaagaac gtatgcggcc acgctgtcgt ccatgtcggc gaccccgaca
      541 aggcgatggc cgaagccgat ctcgtggtcg aggaagttta cgaaaccaag atgatccatc
      601 cgcagtattt ggagccgcgc attgccgcgg cgcagatgga acccaacgga cggctgacgg
      661 tttgggccaa cgcccaagcg ccgttccccg tacgccagga aacggcgaag atgctgggcc
      721 tgcgattgaa tgacgtgcgc gtcatctccg ccggcttggg cggcggattt ggcggcaaag
      781 gcagcggcat cacttcaggc gccgccgtcg agccgatctg cggcctattg gcgatcaaag
      841 ccaagcgtgc cgtaatgatt gtcctcgaca aggccgagga aacgatctcg acaacgattc
      901 gctccggcgc gcgcatgtac atcaaaaccg gcgtgaaaaa agacggcacg attctcgccc
      961 gcaccggcaa agttttctac gacgccggcg gctactccgg attcggcgtt caggccggcg
     1021 gccgctgcac caacatgctc ggcggctggt acctgatgcc caactgccac atcgaaggct
     1081 ttacggtcta caccaacaaa caggtttgcg gcccggtgcg cggccccggc ggcccgcagg
     1141 ccgcgtttgc ggtcgagtcc cacatggact ccatcgccgc caagctcggc atggacccga
     1201 cggaatttag actgaaaaat acgccaaaag ccggcgacaa gatcgtcggc gtggccaagc
     1261 tgcgcgatgt atccctcggc gagacgatca agattgccaa ggaaaaaatc ggctggggca
     1321 cggtcaaact ggaaaaaaac cagggcatcg gtctcgccac cggctcgtgg atcgagagcg
     1381 ctggtcccgg cggcggttcg atcgtaaaag tgaatgaaga cggctccgtg acggtacaca
     1441 tcggcaagat cgacatgggc accgtccctg gtttcggtat tcccatgatt gttgccgagg
     1501 agctcggtat cccggtgagc gatgtcactg tcgtcaatgt cgataccgac gcaagtccgt
     1561 gggacgccgg cacggtcggc agccggggca tcctggtttc cggcggcgcg tcgcgcctcg
     1621 ccgccatcga tgcgcgcact cagctgttta aaatggccgc gggcaaactg gaagcgagcc
     1681 cggacgatct ggagatcgtc gacaagcaga tccgggtcaa aggcacgccg gcgaaaacca
     1741 tagcgctggc gacggtcgcc accgacgccc acaacgtcat cggcgaagtg atcggccgcg
     1801 gctactgcga taacctggca ttggtcgccg aagaaaaggc ccacggcagc tcgcagccat
     1861 tcaccaccca tgcctgcatc gtcgaagtcg acaccatgac cggcaacgtg aaaatcttga
     1921 aatacgtcgc cgtccacgac atcggccagc cgattcattt ggccgccgtc gaaggccaga
     1981 tcgaaggcgc gacagcgatg agcatcggcc aggcgctgtg cgaacaagtc gtcttcgaca
     2041 aagaaggcaa gacactgaac ccaagcttcg tcgactatct gatgccgacc atcaacatga
     2101 tgccgcgcat cgagacgact ttggtccacg gctaccccgg cgccggcccc tacggcgcca
     2161 aaggcgccgg cgaaatcgcc tgcgtcccgc caatggcctg catcgccaac gcgatctaca
     2221 acgcgaccgg cgtgcgggtt acaaaactac cgctcagtcc ggagaatgtg ctgcgggcgt
     2281 tgaaggaggc ggggaaggcg tagagcaatt ataaaacttt taaagggaaa aagggcaggt
     2341 ctcagacctg cccttttatt tctacttcaa ttttggattt cggaattgca gctatgcaat
     2401 caaggaagac aaaatgaaga ccaccttcgc aatcacagcg ctcgccctcg agttggtaac
     2461 gtctccggtc gacgctcaga acgtaaaaat cacgccggtg ggctcgcacc ccggcgagct
     2521 ctgcgcgaac gatcgcgcga ccatattcga agatctgacc ggcgttcgca tcctctacga
     2581 cgtagcctac aatctcaccg gcaccgacga cccgcggctg ggcgagatac atctgattct
     2641 gttgagccat cttcacgggg accacatggg cgactagaaa ctgaaggcgc tgggcgccgg
     2701 cacgtgcgcg aactcggaca gggtgccggc aggtccgaat tccataacag ccgaagtcgc
     2761 cgcagcgaaa aacgcagcgc tggtgatgac ccgaaacatc agtgctttga tcgccgcgag
     2821 aatccagaat atccgcggcg gcaagccgat aaccttctgt ccgcaagttg atggtgcgat
     2881 caatgtcccg gtcgccgagg tgtgccgctc gagcacggat ctcggcggtg tcttcatcgc
     2941 gaaggctcca accgcatcac aaggtgtgga aattacgggg acacgcctga gttttggctt
     3001 attacaccgg tagtcgactt cgccaattcg gagcagatca tagggtcatt tttacttttg
     3061 atcattgtcc atcttgaacc agcggctcgc acctgaaacc
//
LOCUS       DASQKS010000099         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1735970, whole genome shotgun sequence.
ACCESSION   DASQKS010000099 DASQKS010000000
VERSION     DASQKS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735970"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VE170_02675"
     CDS             complement(<1..1652)
                     /locus_tag="VE170_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYJ14363.1"
                     /translation="MEPLADTILGVTIFSGLSREDVAKILGKLEERSFTAGETIIRQG
                     DQGDAFYLIQTGAVQVVVDGGAGNSEIIAILGPRDWVGEMALFSGEPRSATIVAVKDS
                     TLWRLSRQEWDDLIEKHPSLLLQCCATLSKRLNFVDRQYSTGREAFKSLAEEFYAGRS
                     PRQQQFFRQASLMSVMDGATVEQLFQSKTVTRVLAELTKSQSPLVRHLDADRIEFHSF
                     FKDFLHDKLLAIDGGEVKQQLHGTFAKRYAQMGDWQQAIHHAAEVPDWPEVIKLLKAQ
                     TTESLKAAAQLVRNTLERLPREYFIADTALVNLKSDILVHLGDLPGALRAYQEVLAQR
                     AAAGEAVASYRRIAETLARRKEYGQAIGQLRSALSLIERDMTLPEHYSDTAHEPVALT
                     FDGEGSSAVASRRWRFSAAALKSATLSKWFGALLGLGVGGYLWFATPEIGLEPSGTKL
                     LGLIALTLIFWVFRTLPDYGVALMFAMIVILTKLESATTVMGGFASTTWFMTLGVLGL
                     GAAITGSGLFYRLSLHLVRLFPLSFHWQIIATGIMGVVVMAL"
     gene            complement(1675..2667)
                     /locus_tag="VE170_02680"
     CDS             complement(1675..2667)
                     /locus_tag="VE170_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14364.1"
                     /translation="MKRPSKSLWPAVQLFALLFFLSAAAEVQSQINTRIGVLLPELGR
                     AQSQSLKGLHEELQRLGYIDRQNLIVETRNAKGDRGALRPAADDLVARKVAVIFTTGT
                     RATTAAAGASAEIPVVFVHPGDPVAAAMVKNLNEPARNLTGVAGYAVEKTEKRLELLQ
                     ELIPELREVHIFFDSNNNFSRDNFALAQAAAKKLGLSVAPHGVKAADELKTTLSAMRI
                     APGAALFHVPDDLVEGEAEFIFTTARQKRLPSMFNEESWAIQGATAAYGPSYLEMGRR
                     AGAIIGQLLKNPKAALPAIQRADKFDFIVNYRTANFMGMKIAPTVLKKADKVIR"
     gene            complement(2664..>3267)
                     /locus_tag="VE170_02685"
     CDS             complement(2664..>3267)
                     /locus_tag="VE170_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ14365.1"
                     /translation="RSSGALWRTLQQLTQSNLFPVTFDELKGKTEIHSLSGGEVLRFS
                     APGARLMLRKTPSLRRNDDGQLSITAHKSPAHPREGVLLYRVEYKGKTLVYATDVEEQ
                     PGGYADVIEFARGADLLIHDAQYLDGEYHSKTKSKKGWGHSTVEMAAAVAVKAQVKQL
                     VLFHHEPQHDDRVMRRIERLGKKLFAKSIVAYQGLELILP"
BASE COUNT          647 a          942 c          976 g          702 t
ORIGIN      
        1 atcagtgcca tgacgaccac gcccataatg ccggtggcga tgatttgcca atgaaaactt
       61 agcggaaaga gccgaaccag gtggagcgat aggcgataga atagaccgct gccggtgatt
      121 gccgcgccca gtcccaggac gccgagggtc ataaaccaag tcgtgctggc gaagccgccc
      181 atcaccgtgg ttgccgattc caattttgtc agaatgacga tcatggcgaa cattagcgca
      241 accccgtaat ccggcagggt gcgaaagacc caaaagatca gtgtcagcgc gatgagtcct
      301 aggagcttgg tgccagaggg ctccaaacca atctccggcg tggcaaacca gagatagccg
      361 ccaaccccga gacccaacaa ggcgccgaac catttgctta aggttgctga ctttaacgcc
      421 gcggcggaaa accgccagcg gcgtgacgcg accgcgctgc taccctcacc atcgaacgtc
      481 agcgccaccg gttcgtgcgc cgtgtcgctg tagtgctccg gtagggtcat gtcgcgctcg
      541 atcaagctca gagcgctgcg cagttggccg atcgcttgtc cgtattcttt gcgccgtgcc
      601 aaggtttcgg cgatgcggcg gtaactggcg accgcctcgc cggcggcggc gcgctgcgcc
      661 aaaacctcct gataggcgcg caatgcgccg ggcagatcgc cgaggtggac taaaatatcg
      721 ctcttcaaat ttaccaaggc ggtgtcggcg atgaagtatt cgcgcggcag gcgctctaaa
      781 gtgtttctaa ccagttgcgc cgccgctttg aggctttctg tagtctgagc tttaagcagc
      841 ttgatcacct cgggccaatc cggcacctcg gcagcatgat gaatcgcctg ctgccagtcg
      901 cccatttgcg cataacgttt cgcaaacgtg ccgtggagct gctgtttaac ttcaccgccg
      961 tcgatggcga gcagcttgtc gtgtaaaaag tctttgaaga aactgtgaaa ctcgatgcgg
     1021 tcagcgtcga gatgacgcac taggggcgac tggctcttag tgagctcggc gagcacacgg
     1081 gtgaccgttt tcgattgaaa taactgctcg acggtagcgc cgtccatgac gctcattagc
     1141 gaagcctggc ggaagaactg ttgttgtctg ggcgatctgc cggcgtagaa ctcttcggcg
     1201 agcgatttaa acgcttcacg cccggtggag tactgccggt cgacaaaatt taagcgtttg
     1261 ctgagggtgg cgcagcattg caacagcaag ctcggatgtt tctcgatcaa gtcatcccac
     1321 tcttggcggc tcaggcgcca tagggtggag tctttgaccg cgacgatagt cgccgagcgc
     1381 ggctcgcccg agaacaaggc catctcgccg acccagtccc ggggtcctaa aatcgcgata
     1441 atctcggaat tgccggcgcc gccgtcgacc accacctgca ccgcaccggt ctgaatcaag
     1501 taaaacgcat cgccttgatc gccctggcga attattgtct cgcccgcggt aaaactgcgc
     1561 tcctccagtt tgccgaggat cttggcgaca tcctcgcgcg agaggccgga gaagatcgtt
     1621 acgcccagta tggtatcggc gaggggttcc atggtctggt ttacatggtc tggtttagcg
     1681 gatgacctta tcggcttttt tcagcaccgt cggcgctatc ttcatgccca taaagtttgc
     1741 ggtccggtag ttgacgatga aatcgaattt gtcggcgcgt tggatcgccg gcaacgctgc
     1801 tttggggttc ttcaagagtt gtccgatgat cgcgccggcg cgccgcccca tctcgagata
     1861 actcgggcca taggccgcgg tggcgccctg gatcgcccag gattcttcgt tgaacatgct
     1921 cggcaacctt ttttgccgcg cggtggtaaa gatgaactcc gcctcgccct cgaccaaatc
     1981 gtcgggcaca tgaaatagcg cggcgcccgg cgcgatgcgc atggcgctca gagtggtctt
     2041 caactcgtcg gcggctttga cgccatgggg ggctaccgac aggccgagct ttttcgccgc
     2101 ggcctgggcc aaagcgaagt tgtcgcggga aaaattattg ttgctgtcga aaaaaatatg
     2161 cacctcgcgg agttcgggga tgagttcttg taataactcc aagcgtttct cggtcttttc
     2221 cacggcgtat ccggcgactc cggtgagatt ccgcgccggc tcattcaggt tttttaccat
     2281 tgccgcggcc accggatcgc cgggatggac gaagacgacg gggatttcgg cgctggcgcc
     2341 agccgccgcc gtggtcgcgc gcgtgccggt agtgaaaatc accgcgactt tgcgcgccac
     2401 caagtcgtcg gcggcgggac gcagcgcgcc gcggtcgcct ttggcattgc gggtttcgac
     2461 gatcaggttc tggcgatcta tatagcccaa acgttggagt tcttcgtgca accctttgag
     2521 ggactgggac tgggctcggc cgagctccgg caataaaaca ccgatgcggg tattgatctg
     2581 ggattggacc tctgcggcgg cgctgaggaa aaacagcagc gcaaagagct gcactgccgg
     2641 ccaaagtgat ttcgacgggc gtttcacggc aggatcaact ccaatccttg ataagcgacg
     2701 attgatttcg cgaacagctt tttgccaagc cgttcgatgc ggcgcattac ccggtcgtca
     2761 tgctgcggtt catgatgaaa cagcaccagt tgtttaacct gcgctttgac ggcgactgcc
     2821 gcagccatct ccacggtgct gtgtccccac cccttcttcg acttggtttt agaatggtat
     2881 tcgccatcca agtactgggc gtcatgaatc aataggtcgg cgccgcgggc aaattcgatg
     2941 acatcggcgt agccgcccgg ctgctcttcg acatcggtgg catagacgag agttttaccc
     3001 ttgtattcga cgcgataaag caagacaccc tcgcgcggat gggccgggct cttatgcgcc
     3061 gtgatcgata gttggccatc gtcattgcgc cgcagcgatg gcgtttttcg cagcatcagc
     3121 cgcgccccgg gcgcgctgaa gcgcaggact tcgccgccgg acagcgaatg gatttcggtt
     3181 ttgcctttga gctcgtcgaa cgtaaccgga aatagattgc tttgggtgag ctgttgcagc
     3241 gttcgccata acgccccgct cgaccgg
//
LOCUS       DASQKS010000100         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3834879, whole genome shotgun sequence.
ACCESSION   DASQKS010000100 DASQKS010000000
VERSION     DASQKS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834879"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VE170_02690"
     CDS             complement(<1..526)
                     /locus_tag="VE170_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14366.1"
                     /translation="MGVVLGSGDFRFELIEGFGKLPDGWTFHEVAAVAVDNKDQLYCF
                     TRGKHPVIVFDRDGNFLRSWGDGVFKRAHGATIGPDQTIFLTDDGDHTVRQCALDGKV
                     LMTIGVPGKASTYQGGEPFNRCTHVAFSPRGDIYVSDGYGNSRVHKYSPDGKLLFSWG
                     ESGADPGQFNIVHNI"
     gene            complement(622..936)
                     /gene="glpE"
                     /locus_tag="VE170_02695"
     CDS             complement(622..936)
                     /gene="glpE"
                     /locus_tag="VE170_02695"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HYJ14367.1"
                     /translation="MEIRQIQIHEAKKKLDDKNCVFVDIRDPGSYQQAHIPGAIHLHD
                     GNAQEFLQNTPKDLDVIVYCYHGNSSMGATGYFLENGFINVASMAGGFEAWRQVYEHE
                     AA"
     gene            1201..>2142
                     /locus_tag="VE170_02700"
     CDS             1201..>2142
                     /locus_tag="VE170_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14368.1"
                     /translation="MKIKPLQLYSLAAIFQIAGSSGAAMLAPFFMKDHGYSIAAASVP
                     LVVNGIGRVCSDLTSGVMASYISSGVLIVAASAASIVIAASAHFFIESPVVFLGAFTL
                     LGITEAMFALSIRKIAFEQSEPGQQGRGQGQVAAALGIGFSLGPLAAGFVGKRFGAQV
                     LFAFYALPQVIGLILMLSVGAHRSRAITQEAKAQLWREGKTLLTRASFLASLLAVFQS
                     FVMLVGVTRIAFPFLAVNQRGYALDWVGTVVAISRLTDTFGRYTGGWLCDRITAPRVI
                     LLGVAIGIPMLLIQPYGSGVLSLVMPLSIMTMGFGYSN"
BASE COUNT          411 a          652 c          564 g          515 t
ORIGIN      
        1 cgatattgtg aacgatattg aattgcccgg gatcggcgcc cgactccccc caagagaaga
       61 gtaatttccc gtccggcgaa tacttgtgca cccgcgagtt accgtagccg tcggagacgt
      121 aaatatcgcc ccgcggcgaa aacgcgacat gagtgcagcg gttgaacggt tcgccgcctt
      181 gataggtcga ggcttttccc ggcacgccga tggtcataag cactttgcca tccaatgcgc
      241 attgccgcac tgtatgatcc ccgtcgtcgg ttaaaaaaat tgtctgatcg gggccgatgg
      301 tggcgccatg agcgcgcttg aacacgccgt cgccccagga gcgcagaaag ttgccgtcgc
      361 ggtcgaatac aatcaccgga tgcttgccgc gggtaaagca atagagctgg tctttattat
      421 caaccgccac ggccgcgact tcgtgaaacg tccagccatc gggcagtttg ccaaaacctt
      481 cgatcagttc gaaacgaaaa tcaccgctgc caagaaccac gcccataaaa ccctcctgat
      541 caaatcaacg tgctgcccgc ggcaaaccgc gggcagtgat tagattcaat cccagatctc
      601 gactttccat cctcgatgct ctcacgccgc ctcatgctcg tatacctggc gccaagcctc
      661 gaaaccgccc gccatgctgg cgacgttgat aaatccattt tcgagaaaat aaccggtggc
      721 gcccatgctg ctattgccgt gatagcagta gacgatcacg tctagatcct ttggggtgtt
      781 ctgcaaaaat tcttgagcat tgccgtcgtg taaatgaatt gcgccgggaa tatgcgcctg
      841 ctggtacgag ccgggatcgc ggatgtcgac gaacacacag ttcttgtcat cgagtttctt
      901 tttcgcctca tgaatttgaa tctgccggat ttccatcgcg tcctccttct ggattacgat
      961 aggctcaaga ttgccaagct ctcgccaact ttctccaatt actccgcatg cgcaatcgca
     1021 gagggcaggt ttcttatatg cgtctctgcg ttagttgcgg ctatgtctct tatcttcctg
     1081 cttgccgcca ccgcgctgga atgctgccgt tctatcaagc tcgtttcttg ctgtccacag
     1141 tggaaaaacc gaaagccatc gcgtataaac gcgctgactt gacaacttcg atgcgccgcc
     1201 atgaaaatca aaccattgca gctctattct ctggccgcca ttttccagat cgccggctcc
     1261 tcaggcgcgg ccatgctggc gccatttttc atgaaagatc atggctattc gatcgctgcg
     1321 gcgagcgtgc cgctggtggt caacggcatt ggccgggtct gctccgatct cacctccggc
     1381 gtgatggcga gctacattag ctcgggagtg ttgatcgtcg cggcatcggc ggcgtccatc
     1441 gtgatcgcgg catcggcgca tttttttatt gagtcgccgg tcgtattcct tggcgcgttc
     1501 acgcttctcg gtatcaccga agcgatgttt gccctctcca tccgcaaaat tgcatttgaa
     1561 caatccgaac ccggccaaca gggccgcggc caaggtcaag tcgcggccgc cctcggcatc
     1621 ggcttttccc tcggaccgct ggcggcggga ttcgtcggta aacgcttcgg cgcgcaggtg
     1681 ttgttcgctt tctacgcgct gccccaagtc atcggcctca tattgatgct ttcggtcggt
     1741 gcccatcgct cgcgtgccat cacccaagaa gccaaggcgc aactctggcg tgaaggaaaa
     1801 accttattga cccgcgcttc gttcctcgcc tcgttgttgg cggtgttcca gtctttcgtc
     1861 atgctggtcg gcgtcacccg catcgcgttt ccatttttag cggtgaatca gcgcggctat
     1921 gccttggact gggtgggtac cgtggtcgcg atctcgcgcc tgaccgacac cttcggccgc
     1981 tacaccggcg gctggctatg cgaccgcatc accgcgccgc gggtgatctt gctgggcgtc
     2041 gccatcggca tcccgatgct cctcatccaa ccctatggct ccggcgttct gtcgctggta
     2101 atgccgctat cgatcatgac catgggcttc ggctattcta at
//
LOCUS       DASQKS010000101         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_906694, whole genome shotgun sequence.
ACCESSION   DASQKS010000101 DASQKS010000000
VERSION     DASQKS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906694"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="asnS"
                     /locus_tag="VE170_02705"
     CDS             <1..837
                     /gene="asnS"
                     /locus_tag="VE170_02705"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HYJ14369.1"
                     /translation="RKAGLPKKSFCQSILPSSVRGGCCMFNVEAFCLALSKVYTFGPT
                     FRAENSNTPRHLAEFWMVEPEMAFYELEDNMRLAEEFLKYTIRYVLEHCREDLEFFNQ
                     FIEKTVLATLEHVAQSDFGHITYTDAVAELKKSGKTWEFPVEWGCDLQTEHERFLSEE
                     IFKKPVIVTDYPKDIKAFYMRVNDDDKTVRAMDVLVPRVGEIIGGSQREERHDVLTER
                     LRASGLDEKPYWWYLDLRRYGSVPHAGFGLGLERMMMYLTGLKNIRDVIPFPRTPGNA
                     EF"
     gene            975..2231
                     /locus_tag="VE170_02710"
     CDS             975..2231
                     /locus_tag="VE170_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14370.1"
                     /translation="MLAKLSGLYYGWRMVAVASALRVLGAGLHSFGFTVFFLPLSQDL
                     NINRTATSLAFSLARAEGAIEGPIVGHLLDRYGPKPIMLTAALLMGVGYLILSQVDSY
                     ASFLVVYLGIISLVHAGGFMHAPMILINTWFIRQRARAITLNSAAFGLGGVLIAPLLS
                     LVVQAWGWRWGAAVAGITFLVIGIPICSAIRRSPESMGLLPDGDAPPDAAGDGGQRRI
                     VEVEVTLAEALRSFAFWGLVLANGVRNASYHAVSVHFIPLMVWKGLSQYEAAWLLSAF
                     AFLGMASTLVFGWYADHAHKPRLCAMILFGTAIAMLLPLYTGSFWLMALFTMLFATVE
                     ATYTVGWAIAGDLFGRKHFAKIRGYMGLFYTWGGVVGPVMAGAVFDRWQTYQPLLWGL
                     VGVFAGGGIFFASLSHSWQRATGRKQ"
     gene            2409..3632
                     /locus_tag="VE170_02715"
     CDS             2409..3632
                     /locus_tag="VE170_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14371.1"
                     /translation="MPLAPTTRALITLYTSTLMAGMWAMVVPTIPVLAKHFAITPGTA
                     AQLITALSVGRFAGMPISGFVLDRFGARTALIAGPLIACLAAILAAFMPWFTLILIPV
                     FFIGIADSIWVIAREIAGVDLVRADQRGRVLSGFHGINYLGLAFGPLIGGLLTEQIGF
                     RSVFIAYAACAGISTLIGWRLEASYHPIPPMHARPAPIEWSIKAFGQRLRGIRDLFYE
                     IRPELRVTYIVLVLATFTSFAHRVTAQSIVPVFASEVLRLTPKEIGLLFTISGLSVFA
                     MILPAGFVIDKVGRKWATVPSTGIPAIAFLLIPFADSFFQLAVLLSFLGIANGLSLGS
                     LATSTFDVVPLHARGRLQALRRTLAEIGGVSAPLIGGYLVDKFSPGVPFLAYAPLLIL
                     AALLLAVMARETLEK"
BASE COUNT          697 a         1225 c         1091 g          916 t
ORIGIN      
        1 agaaaagccg gcttgccaaa gaagtccttc tgccaatcta ttttgccgtc ttcggtgcgc
       61 ggcggctgct gcatgttcaa cgtcgaggcc ttctgcctgg cgctgagcaa ggtctacacc
      121 ttcggcccga ccttccgcgc cgagaacagc aacacgccgc gccacctagc cgagttctgg
      181 atggtcgagc cggagatggc cttttacgag ctcgaagaca acatgcggct cgccgaagag
      241 ttcttgaagt acaccatccg ttacgtcctc gaacattgcc gagaggatct ggaattcttc
      301 aaccagttca tcgagaaaac agtcctggcg accctcgagc atgtcgccca gtccgacttc
      361 ggccatatca cttataccga cgccgtcgcc gaacttaaaa aatcgggcaa gacttgggag
      421 tttcccgtcg agtggggctg cgatttacaa accgagcatg aaaggttcct ctcggaagag
      481 attttcaaga aaccggtgat cgtcaccgat tacccgaaag acatcaaagc tttttacatg
      541 cgcgtcaacg acgatgacaa aaccgtgcgc gccatggacg tgctcgtgcc gcgggtcggc
      601 gagataatcg gcggcagcca gcgcgaagag cgccacgatg tcttgaccga acggctgcgc
      661 gctagcggtt tggacgaaaa accctactgg tggtacctgg acctgcgccg ttacggctcg
      721 gtgccccacg ccggattcgg tctcggcctc gaacgcatga tgatgtacct gaccggcctg
      781 aaaaacattc gcgacgtcat ccccttcccg cgcaccccgg gcaacgcgga gttttaattc
      841 cgcacggttc aaagttcact gtctgagtcg gatttccgac gttccacccc gcacggttct
      901 tcccttttta gacaacctcg tgctaagtca gaagagattc gtgatttgcc aatacgccag
      961 acaggacaat tttcttgctc gctaaactct ccgggcttta ctacggctgg cgcatggtcg
     1021 ctgttgcctc ggccttgcgt gtgctcggcg ccgggctgca tagtttcggc ttcacggttt
     1081 tctttttgcc gctcagccaa gacttgaaca tcaatcggac cgcgacctcc ctggcatttt
     1141 ccctggcccg cgccgagggt gccatcgagg ggccgatcgt cggtcatctg ctcgaccgct
     1201 acggtcccaa gccgatcatg ttgacggcgg cgttgctcat gggtgtcggt tacttgatcc
     1261 tctcccaagt cgacagctac gccagttttt tagtggtcta tctgggcatc atatcattgg
     1321 tccatgccgg cggcttcatg cacgcgccga tgatactcat caacacttgg ttcattcgcc
     1381 agcgggcccg ggcgattacc ttgaatagcg ccgctttcgg cctgggcggc gtgctcatcg
     1441 caccgctctt gagtctcgtg gttcaagcct ggggctggcg ctggggcgcc gcggtggcgg
     1501 gcatcacgtt cctcgtcatc ggcataccga tctgttcggc catccgccgc tcgcccgaaa
     1561 gcatgggatt attacccgat ggcgacgcgc caccggacgc agcgggagac ggcggtcaaa
     1621 gaagaatcgt tgaagtcgaa gtcaccctcg ctgaagcatt gcgctcgttt gccttctggg
     1681 gcttggtgct cgccaacggc gtgcgcaacg cctcttatca tgcagtgtcc gtgcatttca
     1741 ttccgctgat ggtttggaaa ggcttgagcc aatacgaagc agcctggttg ctcagcgctt
     1801 tcgcttttct cggcatggcc tcgacgttag ttttcggttg gtacgccgac catgcccaca
     1861 aaccgcggct gtgcgcgatg atcttattcg gcactgccat cgccatgctg ttgccgctct
     1921 ataccggctc cttctggctc atggcgcttt tcaccatgct attcgccacc gttgaagcca
     1981 cctacaccgt gggctgggcc atcgccggcg atctgttcgg ccgcaaacat ttcgccaaga
     2041 ttcgcggcta catgggactg ttttacacct ggggcggcgt cgtggggccg gtgatggccg
     2101 gcgccgtgtt cgatcgctgg caaacctacc aaccgctgct ctggggactg gtcggggtct
     2161 tcgccggcgg cgggattttc ttcgccagtt tgtcccattc ctggcagcgc gccaccgggc
     2221 gcaagcaatg acaatggcca tgctgccaca gcggtcgtgc cctcggcgga ttcgatcgcc
     2281 gcgaaagacc cgcagctttc gttgccctcg cgaataattt ttcgccgcta tttcgtgtct
     2341 cagtattgaa aagtgatgtc taccgaagca ctgcatttga taataaccca gtcctggcaa
     2401 cgaccgccat gccgctcgca ccgacgactc gcgcactgat cacgctttac accagcacgc
     2461 tgatggcggg catgtgggcg atggtggtgc cgacgattcc ggtgctcgcc aaacattttg
     2521 ccatcactcc cggcaccgcg gcgcaactga tcaccgccct ttccgtgggc cgcttcgccg
     2581 gcatgccgat cagcggcttt gttctcgacc gcttcggcgc gcgtacggcg ctgatcgccg
     2641 gaccgctgat cgcctgccta gcggcgatcc tcgccgcgtt catgccttgg ttcacgttaa
     2701 ttttaatacc ggtatttttc atcggcatcg ccgacagtat ttgggtgatc gcccgcgaaa
     2761 tcgccggggt cgatctggtg cgcgccgatc agcgcggccg agtcttgagc ggctttcacg
     2821 gcatcaacta tttgggcctt gccttcgggc cgctgatcgg tggtttgctc accgagcaga
     2881 tcggctttcg ctcggtgttt atcgcctacg ccgcctgcgc cggaatatcg accctgatcg
     2941 gttggcggct cgaagccagt tatcatccga ttccgccgat gcatgcgcgg ccggcaccaa
     3001 tcgagtggag tatcaaagcg ttcggccaac ggctgcgcgg catcagagat ctgttctacg
     3061 agattcgtcc cgagctgcgc gtcacctaca tcgtcctcgt gctcgcgacc ttcaccagtt
     3121 tcgcccaccg ggtgacggct caaagcatcg ttccggtgtt cgccagcgag gtccttcgct
     3181 tgacacccaa ggaaatcggc ctgttgttta ccatctccgg tctgtcggtc ttcgcaatga
     3241 tcttgcccgc cggtttcgtc atcgacaaag tcggccgcaa gtgggccacg gtgccgagta
     3301 cggggatacc tgctatcgcg tttctattga ttcccttcgc cgatagtttt tttcagctcg
     3361 ccgttctgtt gtcgttcctc ggtatcgcca acgggctatc gctcggttcg ttagccactt
     3421 cgaccttcga cgtcgtgccg ctccatgccc gcggccgctt acaggcgctg cgccggactc
     3481 tggccgagat cggcggcgtc agcgcgccgc tgatcggcgg ctatttggtc gataagttca
     3541 gtccaggcgt gcccttcctc gcctacgccc cgttattgat tctcgcggcg ctgctgctcg
     3601 ccgtcatggc gcgcgagacc cttgaaaagt aacctgcgcc ggcagtggtt cgatttggcg
     3661 agatccctct ctccgttcgg gatgacagcg gtcttagtct gtcatttcga gcgcagcgag
     3721 aaatctctct tgctcgagtc accggatcac caacgaaccc tcactgccga ttccgccgat
     3781 agatttccga agatttttgg ctataatgtc gtgatcgaat cttgagacgt atcgccatga
     3841 aatatcaaac cgacgatctc cgaatcaaag agatcaaaga actacttccg ccgggacggc
     3901 tgctggccga catcccgatc ggcgacaaa
//
LOCUS       DASQKS010000102         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3420236, whole genome shotgun sequence.
ACCESSION   DASQKS010000102 DASQKS010000000
VERSION     DASQKS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420236"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VE170_02720"
     CDS             complement(<1..530)
                     /locus_tag="VE170_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14372.1"
                     /translation="MLDGKLRIAPTCYHVLHQVPVMTAHELNFFYDEGLKTADGSLGY
                     EILTDAMVPFGLEKLGISQAMKEKSVDIALDVQSRTVFYQRARGADLYVISGWRNQHT
                     NVWVAPPHIKSLKELKGKRVGISDFNSIRHWAIQIQLKKAGLDLERDIEWVRLGVNAR
                     FHVEAIRGGKVECAPVP"
     gene            complement(560..943)
                     /locus_tag="VE170_02725"
     CDS             complement(560..943)
                     /locus_tag="VE170_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14373.1"
                     /translation="MGKATERAKTGNVTIATVRRSDEARQVAARLEAAGIEWGIVEER
                     RTVLASGGGYRASGIKVQVDRNDAKRAVELLRERRDAAPSPEDRTFHVGAKRQVRVVR
                     WWRTALEAGAILALASLLALGFFVD"
     gene            complement(959..1987)
                     /locus_tag="VE170_02730"
     CDS             complement(959..1987)
                     /locus_tag="VE170_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14374.1"
                     /translation="MGYLKLRCLLALTLSLISFENYCQAQASDPKIVEAAKKEGGEIE
                     AYVTLRTDTAQYIWKMFEAKYPFVKVKQYKADSDKMLQRLATEYRAKKYLVDVLNFGG
                     GFHTQVLIEHGIAGAYLSPETKHFAPAFKEKNGLWTTLYYNPLTIVYNTKILAPQERP
                     KEWFDLLHPRFKGRLAIEQEQVTWYAGMLKRYGVEKGKQFMQGLAKHDPRPEQSSRGN
                     ALLAAGEFAAYIGRGHVAEMFKKKGAPVDWIKNPDPLVVQPATVQIAKHAPHPNSAKL
                     LIDFMLSEQVGELLAKEQRLPGRSNAAGLDPAFKEINADKILPLSMEEVQANFRKYLE
                     EYRTYFGK"
     gene            complement(2058..2921)
                     /locus_tag="VE170_02735"
     CDS             complement(2058..2921)
                     /locus_tag="VE170_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ14375.1"
                     /translation="MANFETILFEKQRCGVLITFNRPALRNALSQRMKDELRAALELA
                     RDDEAIRAVVLTGAGGVFSSGDDMNESVAGKPAWPYAIAEGSALADVYNRLRDRARQE
                     KIDEQLYRWQFPKPIIAAVSGWCLGSASWLALSSHLTIAADDAVFGQPQVRHSDGTDF
                     IWTVLGKFKNALRYGLTGDTVDAKEALRLRLVNKVVAKDRLLDECFQLVERIALVPPE
                     TVKINLQKATSGYEMMGLGKAWSLNAELSAMAQLSRREEFYQPLEAAREHGGWKAFFA
                     TRDAPFRDQKN"
     gene            complement(3037..>3731)
                     /locus_tag="VE170_02740"
     CDS             complement(3037..>3731)
                     /locus_tag="VE170_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ14376.1"
                     /translation="STGMDQGGGAAHRRDMDWPYGVRAGENAADFIDNYRGHEAIFMR
                     IFECSKPVIGAINGWAMGVGSWMSLFTHITIASEQAVFAQPEVRHGSNTGFIWTLLAG
                     FKDGLRYSLTGDHIDAQEALRIGLVNQVVPGDRLLDECFAIVERIAMVPPETVKLNLQ
                     LATMGMQMMGFKDAWTMDGQLSAAAHTLLREELRRPLDEKRKSEGTKAYLQMRDGPFQ
                     PEPFGPRAKRRE"
BASE COUNT          744 a         1164 c         1000 g          823 t
ORIGIN      
        1 ggcaccggcg cacattcgac tttgccaccg cgaatggctt ccacgtgaaa tcgcgcattc
       61 actccgagcc gcacccattc gatgtcgcgc tccagatcca aacctgcttt tttcaactga
      121 atctgaatcg cccagtggcg aatgctgttg aaatcgctga tgccaacccg cttgcccttc
      181 aattccttga gggacttaat atgcggcggc gcgacccaga cgttggtatg ctggttgcgc
      241 cagccggaga tgacgtagag atccgccccc cgggcgcgct ggtaaaacac cgtgcgcgac
      301 tgcacgtcga gggcgatgtc caccgatttt tccttcatcg cctgggagat gccgagcttt
      361 tccaagccga acggcaccat cgcgtcggtg aggatttcgt agccgaggga accgtcggcg
      421 gtcttaaggc cctcgtcgta gaaaaagttc aattcatggg ccgtcatcac cggcacttga
      481 tgcagcacgt ggtaacaggt cggcgcaatg cgcagctttc catcgagcat tgaatctctc
      541 tcttcctata aataaaaatt taatcgacaa aaaaccctag cgccaacaac gacgccaagg
      601 cgagaattgc gcccgcttcg agagccgtgc gccaccatct aaccacccgc acttgacgct
      661 tggcgccgac atggaacgtg cgatcctccg gcgatggcgc cgcgtcgcgc cgttcacgca
      721 acagctcgac ggcccgcttg gcgtcgttgc ggtcgacctg cactttgatg ccgctggcgc
      781 ggtacccgcc gccgctggcg agcaccgtcc gccgttcttc gacgatgccc cattcgatac
      841 ccgcggcttc cagccgcgcc gcgacctggc gcgcttcgtc ggaccgtcgc accgtggcga
      901 tcgtcacatt accggttttt gctctttccg ttgccttgcc catttttcac ccgctcggtt
      961 acttcccgaa atatgttcta tactcttcca aatattttcg aaagttcgcc tgcacctctt
     1021 ccatcgataa aggcaaaatt ttatcggcgt tgatctcttt aaaagccgga tcgaggccgg
     1081 cggcattgct gcgccccggc aaacgctgct ctttggccaa gagttcgcca acttgttccg
     1141 ataacatgaa gtcgatcaac agtttggctg agtttggatg gggcgcgtgc ttggcgatct
     1201 gcaccgtcgc cggctggacg accagcggat cgggattttt gatccagtcc accggcgcgc
     1261 cttttttctt gaacatctcc gccacatggc cgcggccgat gtaggcggca aactcgccgg
     1321 cggcgagcag cgcattaccg cgcgacgact gctcgggacg cggatcgtgt ttggccagtc
     1381 cctgcatgaa ctgtttgccc ttctccacgc cgtagcgctt caacataccg gcgtaccaag
     1441 tgacctgctc ctgctcgatg gccaagcggc ctttgaagcg cggatgcaag agatcgaacc
     1501 actctttggg ccgttcttgc ggcgcgagaa ttttggtatt gtaaacgatc gtcagcgggt
     1561 tgtaatagag cgtcgtccac agaccgttct tctctttgaa cgccggcgca aagtgtttcg
     1621 tctccggcga caggtaagcc ccggcgatgc catgctcgat caatacctga gtgtgaaagc
     1681 cgccgccaaa attgagcaca tcgacgagat acttctttgc gcgatattcc gtggccaagc
     1741 gttgcagcat cttgtcgctg tcagccttgt actgcttcac tttcacgaag ggatatttgg
     1801 cttcgaacat cttccaaata tattgcgccg tatcggtgcg cagggtgacg taagcctcga
     1861 tctcgccgcc ttccttcttc gccgcctcga cgatcttggg atcgctcgcc tgcgcctgac
     1921 aatagttctc aaaggagatg agcgacagag taagcgccag caggcaacgt aacttcaagt
     1981 atcccatcag catctccttt ctcgatgcga aacgatctta actttttcaa atatttgacg
     2041 gctgtaacta atcctgtcta attcttttga tcgcgaaacg gcgcatcgcg agtggcaaaa
     2101 aatgctttcc aaccgccgtg ctcgcgcgcc gcttccaagg gctgataaaa ctcctcccgg
     2161 cgcgacagct gtgccatcgc cgacagttcg gcattgagcg accaagcctt gccaagaccc
     2221 atcatctcgt acccggaagt ggctttttgc aaattgatct ttacggtctc cggcggcacc
     2281 agggcaatac gctcgaccag ctggaaacat tcgtcgagca gccggtcttt cgccacgact
     2341 ttgttgacca ggcgtaagcg caacgcttcc ttggcgtcga cggtatcacc ggtgaggcca
     2401 tagcgcagag cgttcttgaa cttgcccaaa acggtccaga taaaatccgt gccatcgcta
     2461 tggcgcacct gcggctgacc gaagacggcg tcatcggcgg caatcgtcag atgagacgac
     2521 aacgctagcc acgaagccga tccgagacac caaccgctga ccgccgcgat aatcggcttg
     2581 ggaaactgcc agcgataaag ctgttcgtcg atcttttcct gccgggcccg gtcgcgcagc
     2641 cgattataaa catccgccag ggcgctgccc tcggcgatgg catagggcca cgccggcttg
     2701 ccggctactg actcgttcat atcgtcgccg ctggaaaaca ccccgcccgc gccggtcagc
     2761 accacggcac gaatcgcttc atcatcgcgc gccagttcca gcgccgctcg caactcgtct
     2821 ttcatgcgct ggctgagcgc attgcgcagc gccggccggt tgaaggtgat caatacgccg
     2881 cagcgctgtt tttcgaacag aatcgtttcg aagttggcca ctatttttct ttcaatttct
     2941 caggggttca agccttcaaa actacgcgaa tgacacctcc gaaaacgact tgaacgcctt
     3001 gaacgttttg aacggctgga actccgcttt cgatatttat tcccgccgct tggcccgtgg
     3061 cccaaacggc tctggttgaa acggcccatc gcgcatctgc aagtaagctt tggttccttc
     3121 ggactttcgt ttttcatcga gcggacggcg caactcttcg cgcagcagcg tgtgcgccgc
     3181 cgccgacaat tgtccatcca tcgtccaggc gtctttgaag cccatcatct gcatgcccat
     3241 ggtcgccagt tggagattga gcttgaccgt ttccggcggc accatggcga tccgttcgac
     3301 aatcgcaaaa cactcgtcga gcagccggtc gccgggcacg acttggttga ccaggccgat
     3361 gcgcaacgcc tcctgggcgt cgatatggtc gccggtgagg gagtaacgca ggccgtcttt
     3421 gaagccggcg agcaatgtcc agataaatcc agtgttggag ccgtggcgca cttccggctg
     3481 ggcgaacacc gcctgctccg aggcgatggt gatatgggtg aacaacgaca tccatgaacc
     3541 cacgcccatg gcccaaccgt tgatcgcacc aatgaccggc ttggagcatt cgaagatgcg
     3601 catgaaaatc gcctcatggc cgcggtagtt gtcgataaag tccgccgcgt tctcgccggc
     3661 gcgcacgccg tagggccagt ccatgtcgcg ccggtgcgcc gcgccgccgc cctggtccat
     3721 gccggtggaa a
//
LOCUS       DASQKS010000103         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2156315, whole genome shotgun sequence.
ACCESSION   DASQKS010000103 DASQKS010000000
VERSION     DASQKS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156315"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VE170_02745"
     CDS             <1..788
                     /locus_tag="VE170_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HYJ14377.1"
                     /translation="DISHVFNYDLPSEVEVYVHRIGRTGRASKTGIAVSLIAPAEHAH
                     LRRIEAFTRQTFLRATLPSVEDIMKRRSEQLTEQVSLWLKRGRINRERTIVETLVAQG
                     HDPLDVAAAALKLARGDEKQRAIAPVAEVEERREAPNHRFQHEPRRGNYRSEQRGRAA
                     AVPAYSREPAKRTAAQRGGKDSHEAGMVRLTLNVGTEHGIGPSDLVGTIAYHADIPGN
                     AIGKIHIEGQKSFVDVPGALVPLVMKQVGKYKIRKQPVKVSLG"
     gene            complement(838..1677)
                     /locus_tag="VE170_02750"
     CDS             complement(838..1677)
                     /locus_tag="VE170_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ14378.1"
                     /translation="MADEKVRFAAALVKARADLDDALFELEKLPAVSQSAVNFATHTL
                     GNFLAVTGVTAELLQLELADHPEPQVHKWIDALLLATEHMTHTVSQLMNAAAGTEAQL
                     RFEKLDLRKLARQSCSAYQRAADRKQIQIIIATNAHAAIPQVWADPVALASVLDNLLS
                     NAVKYSPTGKHVWLSISADEAGVICRVRDEGPGLSLEDQSRLFQRGAKLTPRPTAGEV
                     STGQGLAVAKEWIEKLAGTIWCESQRGRGTCFCFRVPAYRQDLHEPDHVVRSAYWDSA
                     RSA"
     gene            complement(1754..3430)
                     /locus_tag="VE170_02755"
     CDS             complement(1754..3430)
                     /locus_tag="VE170_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14379.1"
                     /translation="MAYDLVIKNGKVIDGSGLPGFHGDVAVSGGRIVEVGKVTGAARQ
                     TLNADGLVVAPGIIDNHTHYDAQVTWDPLCTYSCYHGITTVVMGNCSLAMAPAHREDR
                     AMLANVLSHVEAIPLEAIEAGVNWSWETIPEYLNALDQKLGINVAALIGHSAVRRYVM
                     GEASQDRHATDVEVAAMKAIVREGLEAGAVGISFERNLRHFDWNGRLAPTNLASEEEI
                     FAVAGVADETGRGVIQFGGDRNISTRLAKAISRPVFYGNITQQAVAPDKWRKQLTEAE
                     NMMRQGHRAYQFVMPRPGDLRYTLKTAQHFDAMATWKNVMLLPLEQRKEVFRDPATRA
                     KLHFEAVESPLDPDRAGDFTRRWDLQFVFKPTLAKNQHLIGKSVAEIAQEQNKDVLDA
                     FLDLALEENLETEFERREVNSDQAAMTALLTSPYTIIGQSDGGAHVVFRTDYSYSTYL
                     LSHWVRDKEIMSLEEAIRKLTFVPASLFGFSDRGLVRPGMAADLMVFDPTTISPLEPG
                     VAQDLPGGAKRRKQLARGIEWTLVNGQVLIEHGQHTGAYPGKVARSGTVQ"
BASE COUNT          845 a         1267 c         1207 g          894 t
ORIGIN      
        1 acgatatctc ccatgtgttt aattacgacc tgcccagcga agtggaggtc tacgtccatc
       61 gcatcggccg caccggccgc gccagcaaga ccggcatcgc cgtctctctg atcgcgccgg
      121 ccgagcacgc ccatctgcgc cgcatcgaag cctttacccg gcagacattt ctgcgcgcca
      181 ctttaccgag cgtcgaagac attatgaaac ggcgcagcga acagctgacc gagcaagtgt
      241 cgctctggtt gaagcgcggc cgcatcaacc gtgaaagaac tatcgtcgag acgttggtgg
      301 cccaggggca tgacccgttg gacgtcgccg ctgccgcgct gaagctcgcg cgcggcgacg
      361 agaagcagcg cgccatcgct ccggtagccg aagtcgaaga gcgccgggaa gcgccgaacc
      421 atcgttttca acacgagccc cgccgcggca attaccgcag cgagcagcgc ggccgggcgg
      481 cggctgtgcc ggcttactcg cgcgagccgg ccaagcgcac agcggcgcag cgcggcggca
      541 aggattctca cgaagctggg atggtaagat tgaccctgaa tgtcggcacg gaacatggca
      601 tcggcccgag tgatctggtc ggcaccatcg cttatcatgc cgatatacca ggcaacgcta
      661 tcggcaagat tcacatcgaa gggcaaaaat ccttcgtcga tgtccccggc gccctagtgc
      721 cgctggtgat gaagcaggtg ggcaaataca agattcgcaa gcaaccggta aaagtatccc
      781 tcgggtaaag cagagagcaa tgcttaagag cactgctcga tgtgtgcggc ggttagctta
      841 cgccgatcgg gcactgtccc agtaggcgct tcgaaccacg tgatccggtt catgcaagtc
      901 ttgccggtag gccggcacgc gaaaacagaa acaggttcct cgtccccgtt ggctctcaca
      961 ccagatagtt ccggctaatt tttcgatcca ctccttggcg accgccaggc cttgacccgt
     1021 cgaaacctcg ccggcggtgg gacggggggt taacttggcg ccgcgctgaa atagcctgga
     1081 ttggtcttcg agactcaagc ccggtccctc atcgcgcacg cggcagatga ccccagcctc
     1141 atcggcggaa atgctcagcc agacatgttt gccggttggc gaatacttga cagcgttgga
     1201 aagcagattg tccaataccg aggcgagcgc taccggatct gcccaaacct gtggaatcgc
     1261 ggcatgagca ttggtagcga taattatttg gatttgtttg cggtcggccg ctcgttgata
     1321 agcgctgcac gactggcgcg ccaatttacg caagtccaat ttctcgaagc gcagttgagc
     1381 ttcggtgccg gcggcggcgt tcatgagctg gctcacggtg tgcgtcatgt gttccgtggc
     1441 gagcagcagg gcgtcgatcc acttgtgaac ctgcggctcc gggtgatccg ccaattccag
     1501 ctgcaatagt tcggcggtga cgcccgtgac cgcaaggaag ttgcccaacg tgtgggttgc
     1561 gaaattaacc gcgctctgac tgaccgccgg taatttctcc agctcgaaca acgcatcgtc
     1621 cagatccgct ctggccttta ccagcgccgc agcgaatcgt actttttcat ccgccatcgg
     1681 tcgactccga cttattaccg gtcacggcag aatcggcgct gggatatcgc tgccgatctg
     1741 ccacctgcgc cattcattgc accgtgccgc tgcgagcgac cttgccggga taggcaccgg
     1801 tatgctggcc atgctcaatc agcacttggc cgttgaccaa ggtccactcg atacccctgg
     1861 cgagttgttt gcgccgcttg gctccgccgg gcaggtcctg ggccacgccc ggttccaacg
     1921 gactgatggt cgtcggatcg aacaccatca gatccgcggc catgccgggg cgcacgagcc
     1981 cgcggtcgga aaatccgaag agcgaagccg gcacgaaggt gagcttgcgg atcgcctcct
     2041 ccagagacat gatttccttg tcgcgcaccc agtgcgacag caagtaagtc gagtagctgt
     2101 aatcagtgcg aaaaaccacg tgagcgccgc cgtccgactg gccgatgatg gtgtacggac
     2161 tggtaagcag cgcggtcatg gccgcttgat cgctgttgac ttcgcgccgt tcgaattcgg
     2221 tttcgagatt ttcttcgagc gccaaatcga ggaaggcatc gagcacgtct ttattctgct
     2281 cctgggcgat ttcggcgacg cttttgccga ttaggtgctg gtttttcgcc agcgtcggtt
     2341 tgaagacgaa ttgtaagtcc cagcggcgcg taaagtcgcc ggcgcgatcc ggatccagcg
     2401 gcgactcgac cgcctcgaaa tgcagcttgg cgcgggtcgc cggatcgcgg aaaacttctt
     2461 tgcgttgttc gaggggcagc agcatgacgt ttttccaggt cgccatggcg tcgaagtgtt
     2521 gcgcggtctt cagcgtataa cgcagatcgc cgggccgagg catgacgaat tggtaagcgc
     2581 gatggccctg gcgcatcata ttttccgctt cggtcaattg cttgcgccat ttgtccggcg
     2641 ccaccgcctg ctgcgtgatg ttgccgtaga aaaccggccg gctgattgcc ttggcgagtc
     2701 tcgtgctgat attgcggtcg ccgccaaact ggattacccc acggccggtc tcatccgcca
     2761 cccccgccac ggcaaaaatc tcttcctcgg aagcgagatt ggtcggcgcc aggcggccgt
     2821 tccaatcgaa gtggcgcaga ttgcgctcga aggaaatgcc gacagcgccg gcttccaatc
     2881 cctcgcgcac gatcgccttc atcgccgcca cttcaacatc ggtggcgtgg cgatcctgcg
     2941 acgcctcgcc catcacgtag cggcgcaccg ccgagtggcc gatcagggcg gcgacgttga
     3001 tgccgagctt ttgatccaga gcattgagat actccgggat ggtttcccag gaccagttaa
     3061 ccccggcttc gatcgcttca agcggaatcg cctcgacatg ggacaggaca ttcgccagca
     3121 tggcgcggtc ttcccgatgc gccggcgcca tcgccagcga acaattgccc atcaccacgg
     3181 tggtaatgcc gtggtaacaa gaataggtgc acagcggatc ccaggtgact tgagcgtcat
     3241 agtgggtgtg gttgtcgatg ataccgggcg ccaccaccaa gccgtcggcg ttgagggtct
     3301 ggcgcgccgc gccagtcact ttgccgacct cgacgatccg cccgccgctc acggcgacat
     3361 cgccatgaaa gcccggcagc ccggaaccat cgatcacctt accgttcttg ataaccagat
     3421 cgtatgccat cgttcctcct cgggccgccg gcccggcgtt tcgttgcccg tgcgcggttt
     3481 tcggtcagtc ccgtagcgaa cctatgcgat gcctcagttg ctgtcaatca atatgacagc
     3541 gcccatcgcg ttgttcaatt acaaagattt ggacttgaac ccgccttgtt aaaaatacaa
     3601 ggcgacggtt acgttgatag cactgcactt ttcgcagcac gccgacttct cctttaggtg
     3661 gtgattcctt tggttcctga taccattcgg tggcgggccg ggaaatgcaa ctttgaatta
     3721 cgaaaccagt catccggcgc tggcaaaagc atcttaggtg ccagagcaag tattcgcgtt
     3781 ggaccgacaa gcttccgagc agctcaggca cgcttgccac cactgcctct tgcacccgcc
     3841 gttaggagag atcgttctgc gcggtttctt tgagatgaaa gaacttgttg cccacagcgc
     3901 cggggtaaaa tgcaaatatc ccgctgcatc gcacagctct catcgtgatc gaattaaaat
     3961 tatacaccga cgacagataa gtcgcgccat tccctaaagg tctataatct agacaccagt
     4021 cctgactaaa cgctttattt tgcacattac gcacagagac attctgcctc ggcgtttaat
     4081 tttacggaga accgggtttt ccatcatttg ccgttcctat tgtagatatg gaatggcttt
     4141 ttcgattaac cataacctgt ctcgcgttgc ttcaagactg tggtttatca tgcttcaatt
     4201 cgatgttgac ctt
//
LOCUS       DASQKS010000104         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2585722, whole genome shotgun sequence.
ACCESSION   DASQKS010000104 DASQKS010000000
VERSION     DASQKS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585722"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VE170_02760"
     CDS             <1..858
                     /locus_tag="VE170_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ14380.1"
                     /translation="TLNNKLKAVGAKTPAQIKPLAEKAKAAGDPLTFAMTFPPGTHAM
                     WVRYWLASGGIHPDKDVNLITIPPAQMVANMKVDKMDGFCVGEPWNARAIADGIGYTA
                     VTTQQIWKDHPEKVLAFTEEFAEKNPKTVKAILRAMTESSQYIDKLENRPHMAEVVSK
                     PQYINTQKEVILGRMLGDYDYGDGRKEKDPNYMTFFDRNANFPWKSHGIWWLTQFRRW
                     GMVKGAPDYTGIVNKVMRPDIYREVAKEMSVIAPKEDIKKETLFDKVEFDPAKPEEYV
                     KKFTVSNLA"
     gene            873..1646
                     /gene="ntrB"
                     /locus_tag="VE170_02765"
     CDS             873..1646
                     /gene="ntrB"
                     /locus_tag="VE170_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HYJ14381.1"
                     /translation="MEKRFDAAAFILPLMGISLMLFIWTVISLKIATDLPSPLKTWQE
                     SQQYILDPFFKDGEMNQGILRFAFLSLVRVAKGFFLAIVLGTPLGFILGLSPKFNQTF
                     DPVIQVLRPISPLAWLPLGLVIFKQSEPAALFTIAICAMWPTVINTAMGVRSIATEYL
                     NVGRVLKLSPMKTLTKIMIPATLPYMFTGYRLSLGMAWLVIVAAEMLTGSPGLGGFLW
                     QEYNSLVYSHILLSVLAIGLIGFVLDRLMGVIERRFKSA"
     gene            1654..2493
                     /locus_tag="VE170_02770"
     CDS             1654..2493
                     /locus_tag="VE170_02770"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14382.1"
                     /translation="MAILELKGVSKSFPRHDRGKSQSGKVEVLRDINLSIRENEFVCI
                     VGRSGAGKTTLVSLIAGLIEADTGTILLEGRPIEGPGPDRGVIFQNYSLLPWLSVFEN
                     VYLAVDAVASHLSPAEKRQRTESYIKLVNLGAAMNKVPRELSGGMRQRVAVARGLAME
                     PRVLLLDEPFSALDALTRGTLQEELARIWTETRKTVVMITNDIDEAILLADTIYTLTS
                     APGATLGPAIIVDAVHPRGRMQLTGFAGCQKARRETLKALTPSKGEHGRASAEDPVPA
                     ITH"
BASE COUNT          565 a          691 c          730 g          513 t
ORIGIN      
        1 accctcaaca acaagttgaa ggcggtcggc gcgaagacgc cagcccagat caagccgctc
       61 gccgagaagg ccaaggccgc gggagatccc ctcaccttcg cgatgacgtt cccgccgggg
      121 acgcacgcca tgtgggtccg ctactggctg gcctccggcg gcatccaccc ggacaaggac
      181 gtcaacctga tcaccatccc gccggcgcag atggtggcga acatgaaggt cgacaagatg
      241 gacggcttct gcgtgggcga gccctggaac gcccgcgcga tcgccgatgg tatcggttat
      301 acggcagtga cgacgcagca aatttggaaa gatcatccgg aaaaagtcct ggcgttcacc
      361 gaggaatttg ccgagaaaaa tccgaaaaca gtgaaagcaa tactgcgcgc catgacggag
      421 tctagccagt acattgacaa gttggagaat cgcccgcata tggccgaagt ggtatcgaaa
      481 ccgcagtaca tcaacacgca gaaagaggtc attctcggcc gcatgttggg agattacgat
      541 tacggcgatg gacgcaagga gaaagacccc aactacatga ccttttttga tcgcaacgcc
      601 aattttccgt ggaaatcgca tggtatctgg tggctgacgc aattccgccg ctggggcatg
      661 gtgaaaggtg cgccggacta caccggcatc gtcaataagg tgatgcgtcc ggacatctat
      721 cgcgaagtgg ccaaagagat gagcgtcatc gcacccaagg aagatatcaa gaaagaaacc
      781 ctcttcgaca aggtcgagtt cgatccggca aagcccgaag agtatgtgaa aaaattcacc
      841 gtcagcaatc tggcttagag gctataggcg ccatggaaaa acgtttcgac gcggcggcat
      901 ttattttacc gctcatgggt atctctctca tgctgtttat atggacggtg atcagtctta
      961 aaatagccac cgacctgccg tcacccctca agacttggca ggaaagccaa caatatattc
     1021 tcgatccatt ttttaaagac ggcgagatga atcaggggat attgcgcttc gcatttctaa
     1081 gcctcgtgcg ggtcgccaag ggatttttcc tggcgatcgt cctcggcacg ccgctcggat
     1141 ttatcctggg cctttcgcca aagttcaatc aaacctttga cccggtgatt caagtgctgc
     1201 ggccgatctc gccgctggcg tggttgccgc ttggcttggt tatcttcaag cagtccgagc
     1261 cggcggcttt atttaccatc gccatatgcg ccatgtggcc gactgtgatc aacacggcga
     1321 tgggcgtgcg cagcattgcc acggagtatt taaacgtcgg ccgcgtgctc aaactgtcgc
     1381 cgatgaaaac gctcacgaag attatgatcc ccgcgacgct tccctacatg ttcaccggct
     1441 atcgtcttag tctcggcatg gcctggctgg tgatcgttgc cgccgagatg ctcaccggtt
     1501 cgccaggtct cggcggcttt ctctggcagg agtataacag tttggtctac tcgcatattc
     1561 ttctatccgt actggccatc ggattgatcg gcttcgtact cgaccggctg atgggcgtaa
     1621 tcgagcgccg ttttaaatcg gcttaggcgc aacatggcaa tccttgaact caagggcgtg
     1681 agcaagagtt tcccgcgtca tgaccggggg aagtcgcaga gcggcaaggt cgaagtgctg
     1741 cgcgacatca acctgtcgat tcgtgaaaat gagttcgttt gcatcgtcgg ccgatcaggc
     1801 gcgggcaaga ctacgttggt ctcgctgata gccggcctca tcgaagccga cacaggaacg
     1861 atcctgctcg aaggcagacc gatcgaagga ccgggaccgg accgcggcgt gatatttcag
     1921 aattattctt tgctgccttg gctcagcgtg ttcgagaacg tttatcttgc ggtggacgcg
     1981 gtggcgtcgc acttatcgcc cgccgagaaa cggcagcgca cggagagtta tatcaagtta
     2041 gtcaacctcg gcgctgccat gaacaaggtg ccgcgggagc tttcgggcgg catgcgccag
     2101 cgcgttgcgg tggcgcgcgg attggcgatg gagccgagag tcttgctctt agacgagcca
     2161 ttcagtgccc tcgatgcact gacgcgcggc acgttgcaag aagagctggc gcgcatctgg
     2221 accgagacgc gcaagacggt ggtgatgatc accaacgaca tcgacgaagc gattttgctt
     2281 gccgatacga tttacacttt gacgagcgca ccgggagcga cgcttggacc ggcaatcata
     2341 gtagacgcag tccatccgcg cggtcggatg caattgactg gctttgccgg ctgtcaaaaa
     2401 gcccggcgcg aaaccctcaa agctctcact ccgagcaagg gcgagcatgg ccgggcgagc
     2461 gctgaggacc cggtgccggc gattacccac taggcaggc
//
LOCUS       DASQKS010000105         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_479102, whole genome shotgun sequence.
ACCESSION   DASQKS010000105 DASQKS010000000
VERSION     DASQKS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479102"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1016
                     /locus_tag="VE170_02775"
     CDS             27..1016
                     /locus_tag="VE170_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYJ14383.1"
                     /translation="MDFVRTLKTLADPLRLRMLAAVAEEELTVGEVQEVVASMQSSVS
                     RNLAMLREAGFVRDRKEGTSVYFSVRADMPEPACELFKSLQTKLGEIPEVKKDHARLE
                     ECRRRRLHRSQNYFESVAGDWERIRRSYFDDRVTSLAIEKLLPRKLMLADIGCGTGSL
                     TFELARFARKVIGVDLSSEMLRRARAIARERELANVEFRQGDVLKLPLESHSLDAAFC
                     VMVLHFLPDPARAIAGLCRVVRPGGSVILVDLVEHKQDWMREQMAHQWLGFDRGSVED
                     WFRAAGAPSLDYELTGSFAGEKIARNGNRPVEIFVARATLPGKPASSRSRKHS"
     gene            1016..>4204
                     /gene="metH"
                     /locus_tag="VE170_02780"
     CDS             1016..>4204
                     /gene="metH"
                     /locus_tag="VE170_02780"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYJ14384.1"
                     /translation="MNQAEEILKKLLSQRIVILDGAMGTMIQSHKLSEQEFRGERFAG
                     HRKDLKGCNDLLCLTQADLIADIHAEYLEAGADIVETNTFNSTSISMADYQLESVVYE
                     INVAGAQAAKKAAERVMAKDPARVCFVAGAIGPTNRTCSISTDVNSASTRGVTFEELV
                     DAYYEQARGLIDGGADILLVETIFDTLNSKAAFFAIAKLFDDRKISLPLMASVTFIQP
                     GSNRGVTGQTVEGFWNSISHVPLLSVGMNCALGPKEMRPLMEELAHIAPIYISTYPNA
                     GLPDPLLPTGFPETPESLAPQLREWAANGWLNIVGGCCGTTPAHIRQIAESVNGLAPR
                     PLPQVEPFLRLSGLESLTVRPESNFVNIGERTNVTGSPVFAKLVLAGDYEKAVSVARQ
                     QVEGGAQIIDVNMDEGMLDSKAAMEKFLRLIAGESDIARVPVMVDSSKWEVIEAGLRC
                     LQGKGIVNSISLKEGEEKFIAQARLIRRYGAAVIVMAFDERGQADTYERKIEVCARTY
                     KILTEQVGFPPQEIIFDPNILTVATGMEEHNNYAVNFIEATRWIKDNLPLAKVSGGVS
                     NISFSFRGNNPVREAMHSAFLYHAIKAGMDMGIVNPGMLSVYEQIPADLLELVEDVLL
                     NRRADGTERLVTFADTVKEKGKVAVKDDSWRDGTVEERLSHALVKGLTDFIDHDVEEA
                     RQKYGKPLHVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLTPYLEAEK
                     LRAQDTSKRTKLVFATVKGDVHDIGKNIVGVVLSCNNYDVVDLGVMVPAEKIIDTAIE
                     EKADIIGLSGLITPSLDEMTHVASEMTRRGLTIPLLIGGATTSKRHTAVKIAPSYQHE
                     TIHVIDASRAVPVVGALMKPETRQALDLQNRKEQADIREQFENRAGTQLLSYDEALRH
                     KLHTEWDDHHIAVPEFTGRRVIKDFPLSELVPFIDWSPFFHTWEMRGRYPDLLSDPAR
                     GPAARELFANAQELLKKIVDEKLFTAHGVYGFYPANSDGDDIVLYSDVTRSKQLTRFH
                     TLRQQKDNQRGKPQLALADFIAPHDSGHVDYLG"
BASE COUNT          908 a         1200 c         1247 g          849 t
ORIGIN      
        1 tataatcata gatgttgtat tgagctatgg atttcgtacg cacactcaaa accttggccg
       61 atcccttgcg cttgcgcatg ctcgcggctg ttgccgagga agaattgacc gtcggcgagg
      121 tgcaagaggt ggtggcatcg atgcagtcgt cggtatcgcg caacttggcg atgctgcgcg
      181 aggcgggctt cgtgcgggat cgcaaggaag ggaccagtgt ttatttttcg gtccgcgccg
      241 acatgccgga gccggcgtgc gaactattca agtctctcca aaccaagctt ggtgaaatcc
      301 ccgaggtcaa aaaagatcac gcccgcttgg aagaatgccg tcgccgccgc ctgcaccgtt
      361 cgcaaaatta tttcgaatcg gtggccggcg actgggagcg tatccgccgg agttacttcg
      421 acgatcgcgt aacctcgctg gcgatcgaga aattgctgcc gcgcaagctt atgttggccg
      481 atatcggttg tggcaccggc agtttgacct ttgagctggc gcgtttcgcg cgcaaagtca
      541 tcggtgtcga tctatccagc gaaatgctgc ggcgcgctcg cgccatcgcc agggagcgag
      601 agttggccaa cgtcgaattt cgccagggcg acgtgctcaa gctgccgtta gagtcgcaca
      661 gtctcgatgc cgcgttctgc gtgatggtgc tgcattttct gcccgatccg gcgcgggcga
      721 ttgccggtct ttgtcgcgtc gtccggcccg gcggctcggt gattctcgtc gatctggtcg
      781 aacacaagca ggattggatg cgtgagcaga tggcacacca gtggctcggc tttgatcgcg
      841 gttccgtcga ggattggttt cgcgccgcgg gggcgccgtc gctagattac gagctaaccg
      901 gttcttttgc cggcgagaaa atcgcccgca acggcaaccg gccggtggaa atcttcgtcg
      961 ctagagcgac cttgccaggc aagccggcat caagtcgaag caggaagcat agctaatgaa
     1021 ccaggccgaa gagatattaa agaaactatt gtcccaacga atcgtcattc tcgacggcgc
     1081 catggggacg atgatccagt cccataagtt gagcgaacaa gaatttcgcg gcgagcgctt
     1141 tgccgggcac cgcaaagatc tcaaggggtg caacgatctt ttgtgtttga cccaagccga
     1201 tttgatcgcc gacatccacg ccgagtacct cgaagccggc gctgacatcg tcgagaccaa
     1261 cactttcaat tcgacttcga tctcgatggc ggactaccag ttggagtcgg tggtctatga
     1321 aatcaacgtc gccggcgcgc aggcggcgaa aaaagccgcc gagcgggtca tggccaagga
     1381 tccggcgcgt gtttgtttcg tcgccggcgc catcggcccg accaatcgca cctgctcgat
     1441 ctccacggat gttaatagcg cgtcaacccg cggcgtgacc ttcgaagaat tggtcgacgc
     1501 ctattacgag caggcgcgcg gcctcatcga tggcggcgcc gacattctac tcgtcgaaac
     1561 cattttcgat acgcttaact ccaaggcggc gttttttgct atcgccaagc tatttgatga
     1621 tcgcaagatt tcacttccgc tgatggcatc ggtgactttc atccagcccg gaagcaaccg
     1681 tggcgtcacc ggccagaccg tagaggggtt ttggaattca atttcccatg tgccgctgct
     1741 cagcgtcggc atgaactgcg cgctcgggcc gaaggaaatg cgcccgctga tggaagaact
     1801 ggcgcacatc gcgccgattt atataagcac ttatcccaat gccggattgc ccgatccgct
     1861 gttgcccacc ggttttcccg aaacccccga atcgctggcg ccacaattgc gcgagtgggc
     1921 cgccaacggc tggctcaaca tcgtcggcgg ctgttgcggc acgacgccgg cgcatattcg
     1981 ccagatcgcc gagtcggtaa atggtttggc gccgcggccg ctgccgcaag tcgaaccttt
     2041 cctgcgcttg agcggtctcg aaagcttgac cgtgcggccc gagtcgaatt tcgtcaacat
     2101 cggcgagcgc accaatgtca ccggctcgcc ggtcttcgcc aagctggtgc tcgccggcga
     2161 ttacgaaaag gcggtcagcg tggcacgcca gcaggtcgag ggcggcgcac agattatcga
     2221 tgtcaacatg gacgaaggca tgctcgattc gaaagcggcg atggagaagt ttctcaggct
     2281 aattgccggc gagtccgaca tcgcccgggt gccggtgatg gtcgatagct cgaaatggga
     2341 agtcatcgag gccggtctgc gctgcttgca gggcaaaggc atcgtcaatt cgatcagctt
     2401 gaaagaaggc gaagagaagt ttatcgccca agcaagactg atccgccgct acggcgccgc
     2461 ggtcatcgtc atggccttcg acgagcgcgg tcaggcggat acctacgaac gaaagatcga
     2521 ggtgtgcgcg cgcacctaca agattctaac cgagcaagtc ggattcccgc cccaggaaat
     2581 tattttcgat ccgaatattc tcaccgtcgc caccggcatg gaagagcata acaattatgc
     2641 cgtcaatttc attgaagcga cgcgttggat taaagacaac ctgccgctgg caaaggtctc
     2701 cggcggtgtc agcaatattt cgttttcgtt tcgcggcaac aatccggtgc gcgaagcgat
     2761 gcactcggcg tttctctatc acgcgatcaa agccggcatg gacatgggaa tcgtcaatcc
     2821 cggcatgctc agtgtctacg aacagattcc cgccgacctg ctcgaactgg tcgaggatgt
     2881 cctgctcaac cggcgcgccg acggcaccga gcgactggtc acgtttgcag acaccgtcaa
     2941 ggaaaaaggc aaagtcgcgg tcaaagacga ctcctggcgc gacggcacgg tggaggagcg
     3001 tttatcccac gccctggtca aaggattgac ggattttatc gatcacgatg tcgaggaagc
     3061 ccggcaaaaa tacggcaagc cgctgcacgt catcgaagga ccgctgatgg acggcatgaa
     3121 cgtggtcggc gacctgttcg gttccggtaa aatgtttctg ccccaggtgg tcaagagcgc
     3181 gcgggtgatg aaaaaagccg tggcctattt gacgccctat ctggaagcgg agaagttgcg
     3241 agctcaggat acctcaaagc gcaccaagct ggtgttcgct acggtcaaag gagatgttca
     3301 cgatatcggc aaaaacatcg tcggcgtcgt gcttagctgc aacaactacg atgtcgtcga
     3361 cctcggtgtc atggtgccgg cggaaaagat catcgacacg gcgatcgaag aaaaggccga
     3421 tatcatcggc ttgagcggtt tgattacgcc gtcgttggac gaaatgaccc atgtcgctag
     3481 cgagatgacc cgccgtggtt tgactattcc gctgctgatc ggcggcgcga ccaccagcaa
     3541 gcgccatacg gcggtaaaga tcgcgccgtc ataccagcat gaaaccattc acgtgattga
     3601 cgcctcgcgc gccgtgccgg tggtgggcgc gttgatgaag ccggaaacgc gccaggccct
     3661 cgacctacag aaccgcaagg agcaggcgga tatacgcgag caattcgaga accgcgccgg
     3721 cacgcagctg ctcagttacg atgaagcgct gcgccataaa cttcacaccg aatgggacga
     3781 ccaccacatc gccgtgcccg aattcaccgg acggcgcgtg atcaaggatt tcccgctatc
     3841 agaattggtg ccgttcatag attggtcgcc gttttttcac acctgggaaa tgcgcggccg
     3901 ctatccggat ctgctcagcg atccggcgcg cggcccggcg gcgcgtgaat tgttcgccaa
     3961 cgcccaggag ttgttgaaaa agatcgtcga tgagaaactg ttcaccgccc atggagtcta
     4021 cggcttctat ccggccaaca gcgacggcga cgatatcgtg ctctattcgg atgtcactcg
     4081 aagcaagcaa ctgacgcgtt ttcatacgct gcgtcagcaa aaagacaatc agcgcggcaa
     4141 gccgcagttg gcgttggccg atttcatcgc accgcacgac agcggccatg tcgattacct
     4201 cggc
//
LOCUS       DASQKS010000106         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_479335, whole genome shotgun sequence.
ACCESSION   DASQKS010000106 DASQKS010000000
VERSION     DASQKS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479335"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /gene="gcvT"
                     /locus_tag="VE170_02785"
     CDS             <1..802
                     /gene="gcvT"
                     /locus_tag="VE170_02785"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYJ14385.1"
                     /translation="VDDVIIYRVEPEAYFICVNASNIDKDFAWLSSRAGDGIEVENHS
                     ALYAQLALQGPVAENILQPLIAQPLAALKSFHFVFADVAAIRCMVARTGYTGEPGFEL
                     YCHSDAALGLWDRLMTAGEPHGLIAVGLGARDTLRLEKAYPLYGHELDDSTTPLEAGL
                     EWVVKLAKPAFLGRDRLLQQQQQGVERKLVGIEALGPGIPRGGYGVYKDGVKIGAITS
                     GTKSPSLGKAIALAYVASPEAWIDNRLEVEIRGRQIPVKIVSLPFYRR"
     gene            838..1215
                     /gene="gcvH"
                     /locus_tag="VE170_02790"
                     /pseudo
     CDS             838..1215
                     /gene="gcvH"
                     /locus_tag="VE170_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
     gene            1306..2655
                     /gene="gcvPA"
                     /locus_tag="VE170_02795"
     CDS             1306..2655
                     /gene="gcvPA"
                     /locus_tag="VE170_02795"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HYJ14386.1"
                     /translation="MRYTPHTEADKQRMLREIGCASVEDLYRHVPQSLRDQAAIGLPP
                     GLTELEVRRRMTALAARNNSAADWSFFLGGGIYHHFIPSAVDATISRAEFSTSYTPYQ
                     PEVSQGTLQALFEYQTLICQLTGMEVANAAVYDGASAVAEAVLMSRRIQPSKKRRVLM
                     SRALHPQYRAVVSTYLKNLDDVILEEVPFDAGRGATDFAQLQQLLDERTMCVLLGYPN
                     FFGVIEDPAPIRAACDSAGAHLVTVTSEPLSLALLKPPGAFGADIAVGEGQSLGVPMS
                     LGGPAFGFFACQKKFVRNIPGRLVGETVDSDGRRGFVLTLATREQHIRREKATSNICT
                     SQTLCALAATVYMALLGKSGLRRIAEINLARAHDLQARLSQQAGLEPLFPGPFFNEFV
                     LQSDNLPKLFEDCAAQKIVPGIALEQWYPELKNAFLVCATEMNEREEIDRLVRTSAG"
     gene            2633..4138
                     /gene="gcvPB"
                     /locus_tag="VE170_02800"
     CDS             2633..4138
                     /gene="gcvPB"
                     /locus_tag="VE170_02800"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYJ14387.1"
                     /translation="MSEPAQDKKRSQVAPPKLLFESGSSGRSAIDWAAEGNRVEDAIP
                     AALLRRDIDGFPELGELEVLRHFTGLSQRNFSIESQFYPLGSCTMKYNPKINEVLARL
                     PGFAQLHPMASPEHLQGALALLFELEQMLAEVSGMEYVSLQPSAGAQGELTGLMLIRA
                     CLSKRGDPRKNIIVPDTAHGTNPASSTLCGYDVIQISSNAKGVIDAAAVEKVMDGDVA
                     AIMITNPNTLGLFETNIEAIGQIVHAKGGMVYLDGANLNALMGVAKPGHMGVDVLHMN
                     LHKTFSTPHGGGGPGAGPVGVKAQLRDYLPVPRIIKSGDKFVLEASCPDSVGRVRSFL
                     GNFGVLLRAYTYIISLGGDGLADVSRMALLNANYIRKKLEKSYQLAYNEPCMHECILT
                     DRIQHKNGVSTLDIAKRLLDYGYHPPTIYFPLVVSGALMIEPTETESPETLDGFCDAM
                     LAIAEEAKDDPELVTSAPHSTPVRRLDEARAARKPVLRWVSSRAKNGLPEE"
     gene            complement(4167..5006)
                     /locus_tag="VE170_02805"
     CDS             complement(4167..5006)
                     /locus_tag="VE170_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ14388.1"
                     /translation="MARLNITLTRFFGLSLAIHAAIFCYFILMQKQAWKSRAEPPEIP
                     VTLLPPGERDSPTPPAMMPRAPASAGKKVPATVNRRELSPRANRVRERVRRTTAEKPA
                     AAIETASRGESIRDEQAIGLPVPTSPDTQREPPIEKSVVAERRLPTVKELLPPLTYSS
                     TGGRGGAPVSLNTNDPAYMSYFNRIKHAINQKWEYPELAKRYGLQGTLLLEFAVGASG
                     QIEQLRLVRSSGSQLLDDEALRAIKAAAPFPPIPAWITSTPLPISATMEYHDSRLNYP
                     FTR"
     gene            5098..5283
                     /locus_tag="VE170_02810"
     CDS             5098..5283
                     /locus_tag="VE170_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYJ14389.1"
                     /translation="MFGFGIGELLVVLVIVLILFSNRLPDLGEGIGKSIRKFRKSVNQ
                     ADEVDITPKKKDSDDLP"
     gene            complement(5293..>5903)
                     /locus_tag="VE170_02815"
     CDS             complement(5293..>5903)
                     /locus_tag="VE170_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14390.1"
                     /translation="FAGPELKQKQENNANILAMSSYADQRASIRPYLSLFGLLLIGFS
                     LLAAMTHAKPTAMENLVKNIPFVGATMLKNNHLKDGVLLQSLRTGYQNIQGDREVLLL
                     TGVAFNQNPVVIREVKVTGKVFNLEGKELEKQTIWIGNTISPKIIRGMTAEDIPHLQD
                     LKPLKSFELPPGDSIAFTMVFLKPPKQVKDFSLEVVLADGAI"
BASE COUNT         1258 a         1631 c         1759 g         1255 t
ORIGIN      
        1 ggtcgacgac gtgattatct atcgggtcga gcccgaagct tattttatct gtgtcaacgc
       61 ttcgaacatc gacaaggatt ttgcctggct gtcgagccgc gccggcgacg gtatcgaggt
      121 ggaaaatcac agcgccctgt acgcccagtt ggccttgcag ggacccgtgg cggagaatat
      181 tcttcagccg ctgatcgctc agccgctggc ggcgctcaag tcgtttcact tcgtgtttgc
      241 cgatgtcgcg gcgatacgct gcatggtggc gcgcaccggt tataccggcg agccgggatt
      301 cgagctttat tgtcacagcg acgccgcgct cggcttgtgg gataggttaa tgaccgccgg
      361 ggagccgcac ggtttgatcg ccgtgggact gggcgcgcgc gatacgctgc ggctggaaaa
      421 ggcctatccg ctttatggcc atgagttgga tgacagtacg acgccgttgg aagcgggctt
      481 ggaatgggtg gtgaaacttg ccaaacccgc gtttctcggc cgcgatcgct tactccaaca
      541 acaacagcaa ggcgtggagc gtaagttggt cggcatcgaa gccctgggac ccggcattcc
      601 acgcggcggt tatggcgtgt acaaagacgg cgtcaaaatt ggcgcgatca ccagcggcac
      661 caagtcgccg agtttgggca aggccattgc gttggcgtat gtcgccagcc cggaggcgtg
      721 gatcgataat cgcctcgagg tagaaattcg tggccggcag attccggtaa aaatagtttc
      781 attgccattt tatcgccgtt agcggcgcag ccagacggct catcaaggag gtttgcgatg
      841 gagtttcccg aaggactaaa atattccaaa gaacatgaat gggtgctggt cgaaggcacc
      901 agcgcgacga tcggtatcac cgagtacgcc caagaagagc tgggcgacat tgtttttgtc
      961 gagttgccgg aagtgggtga aaagatcgtc aaggacgatc ccttcggcgc ggtggaatcg
     1021 gtcaaggcgg tgtcggatat ttacgccccg gtgagcggcg aggtgatgga gaccaatgag
     1081 atcctgccgg ataacccgga gacgatcaac gacgatccct acggcgatgg ctggatggtg
     1141 cgcgtggaac tctccgatat cgacgatctc aaggatctga tggacgccga cgaatatgcc
     1201 gagtacgtcg cccagcaaaa agaagacgat gacgacgacg aagaagaaga cgacgaggaa
     1261 gatgacgaag aagaggacga agacaaagac aagtagcccc cgctgatgcg atataccccc
     1321 catactgaag ccgacaaaca gcgcatgctg cgggaaatcg gctgcgcctc ggtggaagat
     1381 ctctatcgcc atgtgccgca gtcgttgcgc gatcaggcgg cgatcggcct tcccccagga
     1441 ttgaccgagt tggaagtgcg ccggcgcatg accgctctgg cggcgcgcaa taactcagcg
     1501 gccgactgga gtttttttct cggcggcggc atctaccatc atttcattcc tagcgcggtg
     1561 gatgcgacca tttctcgcgc ggaattttcg acttcgtaca cgccctatca gcccgaggtc
     1621 agccagggca cgctgcaggc gttgttcgag taccagacgc tgatttgcca actcaccggt
     1681 atggaggttg ccaatgcggc ggtctatgac ggcgcctccg cagtcgccga ggcggttttg
     1741 atgtcgcggc gaattcagcc gtccaagaag cgccgggtgc tgatgtcgcg ggcgctgcac
     1801 ccgcagtacc gtgccgtggt gtcgacctat ttgaaaaatc tagacgacgt cattctcgaa
     1861 gaagtgcctt tcgacgccgg ccgcggcgcc acggatttcg cccaactgca gcagttgctc
     1921 gatgagcgca ccatgtgcgt gctcttgggc tatccgaatt ttttcggcgt gatcgaagat
     1981 ccggcaccga ttcgggcggc atgcgacagc gccggcgccc acctggtgac ggtcacgagc
     2041 gagcccttgt cgctcgcact attaaaaccg ccaggcgcat ttggcgccga tatcgcggtg
     2101 ggagagggac agagcttggg cgtgccgatg agtctcggcg ggccggcttt cggatttttc
     2161 gcctgccaaa aaaagttcgt cagaaatatc ccggggcgat tggtcggtga aaccgtcgac
     2221 agcgacgggc ggcgcggttt tgttttgact cttgccaccc gcgagcaaca tatccggcgt
     2281 gaaaaggcga cgtccaatat ttgcaccagt cagaccttgt gcgccctggc ggccaccgtt
     2341 tacatggcat tgctgggcaa atcgggattg cgccgcatcg ccgagatcaa tctggcccgc
     2401 gcccatgacc ttcaagcgcg attatcccag caggcggggc ttgagccgct ctttcccgga
     2461 cctttcttta acgaatttgt gctccagtcg gacaatctcc caaagctgtt cgaagattgc
     2521 gcggcgcaaa agatcgtccc cggcatcgcc ttggagcaat ggtatcctga gctgaaaaac
     2581 gccttcttgg tatgtgccac cgaaatgaac gagcgagagg aaatcgaccg acttgtccga
     2641 accagcgcag gataaaaaac gctcccaggt ggcgccgccg aaattactct tcgaaagcgg
     2701 ctcatcgggg cgcagcgcca tcgactgggc ggcagagggt aatagggtcg aagacgcgat
     2761 tcccgcagcg ttgctgcggc gagacataga cggcttcccc gagctgggcg agcttgaggt
     2821 gctgcgtcac ttcaccggtt tatcgcaacg caacttctcc atcgagagcc agttctaccc
     2881 gttgggctct tgcacgatga aatacaatcc caagatcaac gaagtcctgg cgcgtctgcc
     2941 cggcttcgcc cagcttcatc caatggcgtc gcccgaacat ctacagggcg cgttggcgct
     3001 gctcttcgag ctcgagcaaa tgcttgccga agtgagcggc atggagtacg tgagtttgca
     3061 gccttccgcc ggcgctcaag gcgaactgac cggtttgatg ctcattcgcg cctgtctgag
     3121 taagcgcggc gatccgcgaa aaaacattat cgttcccgac actgcccacg gcaccaaccc
     3181 ggcgagttca actttatgcg gctacgacgt gatccagatc tcctccaacg caaagggcgt
     3241 gatcgacgct gctgcggtgg aaaaagtcat ggacggcgat gtcgcggcga tcatgatcac
     3301 caatcccaat accctgggac tgttcgagac caatatcgaa gcgatcggcc aaatagtcca
     3361 cgccaaaggc ggtatggttt acttggacgg cgctaactta aacgcgctta tgggcgtggc
     3421 caagccgggc catatgggtg tcgacgtttt acacatgaac ctgcacaaga ccttctccac
     3481 gccccacggc ggcggcggtc cgggagccgg gccggtgggg gtcaaagcgc aattgcgtga
     3541 ctatctgcct gtgccacgca tcatcaagtc cggcgacaaa tttgttttgg aagcgagctg
     3601 tcctgattcg gttggccgag tgcgctcgtt tctcggtaac ttcggcgtgc tgttacgggc
     3661 ctacacctat attatttcgc ttggcggcga cggcctcgca gacgtgagcc gcatggcgtt
     3721 actcaacgcc aactacattc ggaagaaact cgaaaagagc taccaacttg cgtacaacga
     3781 accgtgcatg cacgagtgca ttctgaccga tcgtatccag cacaaaaatg gcgtgagcac
     3841 tctcgatata gccaagcggc tgctcgacta cggctatcat ccgccgacga tttatttccc
     3901 gctggtggtt tccggcgcct tgatgatcga gccgaccgaa accgaatcgc cggaaacctt
     3961 ggatggattt tgcgacgcca tgttagccat cgccgaggaa gccaaagacg accctgagct
     4021 agttacgagc gcgccccatt ccactccggt gcggaggctg gacgaggcgc gagcggcgcg
     4081 caagccggtg ctgcgttggg tgagcagtcg ggcaaagaac ggattgccgg aggaatagtc
     4141 catcgcggct cgagcgtaag cttggctcaa cgggtgaagg gataatttag gcgactgtcg
     4201 tggtactcca tcgtcgccga aattggcagc ggcgtgctcg taatccacgc cggaatcggc
     4261 ggaaacggcg cggccgcttt gatcgcgcgc aacgcttcgt catcgagcaa ttgggaaccg
     4321 gaagagcgca cgagtcttaa ctgctcgatt tgaccgctag cgccgacggc gaactccaac
     4381 agcagcgttc cttgcagacc gtatcgtttc gccagctctg gatactccca cttctggttg
     4441 atggcgtgct tgatccgatt gaaataactc atgtaggcag ggtcattggt gttcaagctc
     4501 accggtgcgc cgccgcgccc gcccgtgctc gaataggtaa gcggcggtaa gagttctttg
     4561 accgttggca gccggcgctc ggcaaccacg ctcttttcaa tcggcggctc gcgttgcgtg
     4621 tcaggcgatg tcggcaccgg caatccgatc gcctgttcgt cgcggatact ttcgccacgc
     4681 gaggccgttt caattgcggc ggcgggtttt tcagcggtgg ttctgcgaac gcgctctcga
     4741 acgcggttgg ctcgtggcga caattctcgc cggttgacgg tcgctggaac ttttttgccg
     4801 gcgcttgccg gcgctcgcgg catcatggcc ggaggcgtgg gtgagtctct ctcaccgggc
     4861 ggcagtagtg taaccggaat ctctggcggc tcggcgcggc tcttccacgc ctgtttttgc
     4921 atgaggataa agtagcagaa aatcgcggca tgaatcgcca gtgaaagccc gaagaagcga
     4981 gtcagcgtga tgtttaatcg tgccacgatg gtataatcta gacaaaatcg gcaaggaaga
     5041 ttcgtcgata atcaattctt accacggcgc gagcgccgtt gggcgtctgg aggaccgatg
     5101 tttgggtttg gcataggcga attattagtg gtgctggtta tcgttctaat attgttcagc
     5161 aaccgtctgc cggacctcgg tgagggcatc ggcaagagca ttcgcaagtt tcgtaaatcg
     5221 gtcaaccaag ccgatgaggt cgatattact ccgaaaaaaa aagattcaga cgacttgccc
     5281 tgacatcgcc catcaaattg cgccgtcggc gagcacgact tccaagctga agtccttgac
     5341 ctgttttggc ggtttcaaaa acaccatggt aaaagctatc gaatcccccg gcggcagctc
     5401 aaaactcttg agcggtttca agtcctgcaa gtggggaatg tcttccgcgg tcataccgcg
     5461 gataatctta ggtgaaatcg tattgccgat ccagatggtt tgcttctcaa gctccttgcc
     5521 ttcgagattg aacaccttgc cggtgacttt cacctcccga atcaccaccg gattctgatt
     5581 gaaggcgacg ccagtgagca gtaatacctc acgatcccct tgaatattct gataacccgt
     5641 gcgcaaggat tgcagcagga cgccgtcctt caagtgatta ttttttaaca ttgtggcgcc
     5701 gacgaagggg atatttttga ccagattctc catcgccgta ggcttagcat gggtcatcgc
     5761 cgccaacagt gaaaagccga tcaataatag tccaaacaag ctcaaatacg ggcggatcga
     5821 ggcgcgttga tcggcgtagg atgacatggc cagtatgttg gcattatttt cctgtttttg
     5881 ctttagctca ggcccggcaa atg
//
LOCUS       DASQKS010000107         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5097877, whole genome shotgun sequence.
ACCESSION   DASQKS010000107 DASQKS010000000
VERSION     DASQKS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097877"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VE170_02820"
     CDS             <1..802
                     /locus_tag="VE170_02820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ14391.1"
                     /translation="PVILAGVDADLARPVMRAAQKAFKEIRLNTKVLKMATAGKQIKV
                     TIEMDKQPRDEIYDRVLVSVGRIPNLADLGLENTKVTKDDKGFIHCNSQQQTEDPNIY
                     AIGDVNGGALLAHRATREGRIAVEAILGEPSAFENIVIPAVVYTDPEVAWCGLTEAEA
                     KEKNIGIKVAKFLWGASGRALTLDHPDGLTKLIIEPETERILGVGVVGAGAGELISEG
                     VLAVEMAATARDIAESVHPHPTLSETFMEAAESFYGTATHTLSRRNES"
     gene            799..1686
                     /locus_tag="VE170_02825"
     CDS             799..1686
                     /locus_tag="VE170_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYJ14392.1"
                     /translation="MTEQPPRLFLIDGSSYIFRAFFALPPTLKTAAGLPTNAIIGFTN
                     LLLKFLKQYRPVYIAVALDAGRMTFRNEMYTDYKGNRPEAPADLIAQFPYFRKVLDAL
                     NLPLLELPGYEADDIIATLCERLANQGLELVVVSSDKDLMQLITNGTKLLDSAKDRWI
                     GENEVMEKFGVAAAQVIQVMGLMGDTVDNIPGVKGIGGKTAGALIQHFLTLENLYDHL
                     DELEQLKLRGTARVRELLKKHKGNAFLSRDLATAKRDVPIDIGLPELLFTGFNYPKIR
                     ALFTELEFPSLIKFIEYGG"
     gene            1827..2990
                     /locus_tag="VE170_02830"
     CDS             1827..2990
                     /locus_tag="VE170_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ14393.1"
                     /translation="MINRQRLQNFLIELIKIDSLSRREYDVAMRLKREMEELGAAVSI
                     DDAGAKVGGNVGNLLAKFPGNAAASQPLLLSAHMDTVVPGEGIVPILEGDILRTDGTT
                     VLGGDDKSGVAIICEVMRVIKENGLPCSAVDVVFTICEEAGLIGAKCLDITSLRARTG
                     LVLDSDSVGFLFTRAPAANRMEFRVHGLEAHAGVCPEKGINAIKVAAEGIAQMQLGRI
                     DHETTANIGVVEGGMAVNIVPNQVVLKGEARSHDSDKLKRQTEHMQRCLEEAAARHWL
                     EFDGKRHQARVEAKIERDYDRMDVPENAPIVQLVHAAARNLHLDVKTHATGGGCDANV
                     LNQKGLVVANLGTGMREIHTVNEWLDLKDLYLSAQMVLEVVKLNATGSLRFAG"
     gene            2994..4439
                     /locus_tag="VE170_02835"
     CDS             2994..4439
                     /locus_tag="VE170_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ14394.1"
                     /translation="MKTEQIDLLLKQERGQFLEFISAFEYRRSSGQKKREEELSRELV
                     RVLSAMANADGGTVLVGVEPDRSVTGIPHDDDELQTLIQAPQNLLRPQLTPACEKIRL
                     GSQQLLKFDVASSMDIHRIAGGRSFYRIAAETPALAAEQIHSLKEAKRNVFYERQQTF
                     NATWFDLDTDLVNQFVNKLHPGEDPQAVLSQTYHLLDNSRAIPCPNMAALLLFAKDPT
                     RWHPRAGIDFVKYDGNERQHGTSLNVVKRMRFEAPLVRLIDEAVGRIKEQVRGRTILH
                     DLFFRERLEYPTFAWQEALVNAVAHRDYSLTGASVEVWMFDDHICVRSPGLPPAPVTL
                     EQLRLQKSIHFSRNPLIVRALADLGFARDMGEGVPRIYQEMETYGLHPPEFAFEGFVF
                     TVTLRNAPVYDEATLRWLSQFANNQVNLRQRRVLAYAYCHGKTFSTTEYERIAEVDRD
                     TAYRDIRLMVKNGIVAPLKPKSRSYRIVEKL"
     gene            4452..5441
                     /locus_tag="VE170_02840"
     CDS             4452..5441
                     /locus_tag="VE170_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ14395.1"
                     /translation="MDFSQLMGRASGHAEARIIQTAVELTIFDALEAADLDTAAVAQN
                     LKLDEQATELLLNALSALGLLEKRGRSFSLTGVSRRHLLRRSPQYVGGMISFDAMLWH
                     DWEKLPEAIRTGLPTRPPNMYQSDARETAVFINAMDSLVKARGDAEILATVLDWSKVE
                     SILDVGSGPATYPIALCENFSQLRATIVDLPGTLEITDRFVRGANLSARIELIAGDYR
                     IDPITGSYDAVFLSNIIHGENYGCNTTLMRKLAANLKPGGRLIVKDHILDDSRSQPHV
                     GAIFSLLMLLTTDGGRCYSLTEIESWMQQAGMNRIEHIELPAPMTSSLVIAWK"
BASE COUNT         1277 a         1665 c         1539 g         1125 t
ORIGIN      
        1 gccggtgatt cttgccggcg tcgatgccga cctcgcccgc ccggtgatgc gcgcggcgca
       61 gaaggcgttt aaagaaatcc gcctcaatac caaagttttg aagatggcca ccgccggcaa
      121 acagatcaaa gtcacgattg aaatggataa gcagccgcgc gacgagattt acgaccgcgt
      181 cctggtgagc gtcggccgca tacccaatct cgccgacctc ggtctagaaa acaccaaggt
      241 caccaaggac gataaagggt ttattcattg caattcccag cagcagaccg aagatcccaa
      301 catctacgcc atcggcgatg tcaacggcgg cgcgctgttg gcgcaccggg cgacccgcga
      361 aggcaggatc gccgtcgaag cgatactcgg cgaacccagc gcttttgaaa atattgtcat
      421 cccagcggtc gtctataccg acccggaggt ggcatggtgc ggtctcaccg aagccgaagc
      481 caaggagaaa aacatcggca tcaaggtcgc taaattcttg tggggcgcct cggggcgagc
      541 gttgactctc gatcaccccg atggcttgac caaactgatt atcgaaccgg agaccgaacg
      601 catcctcggt gtcggcgtgg tcggcgctgg cgccggcgag ttgatcagcg agggggtctt
      661 ggccgtggag atggccgcca ccgcgcgcga tatcgccgaa tcagtgcatc cgcacccaac
      721 gctatcggaa accttcatgg aagccgcaga gtctttctat ggtacggcga cacacaccct
      781 gtcgcggcgc aatgagtcat gaccgagcaa ccgccgcggt tgttcctgat tgacggcagc
      841 tcttatattt ttcgcgcgtt cttcgcctta ccccctactc tgaaaaccgc tgctggattg
      901 ccgaccaacg ctattatcgg tttcaccaac ctcctgctga agtttttgaa gcagtatcgg
      961 ccggtataca tcgcggtcgc attggatgcc ggccgcatga cgtttcgcaa tgaaatgtac
     1021 accgactata aaggcaatcg tcccgaagca ccggccgatt tgattgccca gtttccctac
     1081 ttccgcaaag ttctcgatgc cttgaacctg ccgctgctcg aattaccggg ttacgaggcc
     1141 gacgacatca tcgcgacatt gtgcgaacgg ctggcgaacc aaggtttaga attagtcgtg
     1201 gtttcgagcg acaaggattt gatgcaactg ataaccaacg gaaccaaact tttagacagc
     1261 gccaaagacc gatggatcgg cgagaacgaa gtcatggaaa agtttggcgt tgccgcagca
     1321 caggttatcc aagtcatggg cctgatgggc gacacggtgg acaatatccc tggggtcaag
     1381 ggcatcggtg ggaaaaccgc gggcgcgcta attcaacatt ttttaacttt ggagaatctt
     1441 tatgaccatc tcgatgagtt ggagcaattg aaactacgcg gcacggcccg cgtccgcgag
     1501 ctcctcaaaa agcacaaagg caacgctttc cttagccgcg atctcgccac ggcgaagcgc
     1561 gatgtaccca tcgacatcgg cttgcctgaa ctgctgttta ccggtttcaa ttacccgaaa
     1621 atccgtgcgc tgtttaccga attggagttt cccagcctca tcaaattcat cgaatacggc
     1681 ggctaagccc ggctaccatt ttcgcaaata gcattgccgg cagcttggct ttgaacgctg
     1741 aactgtggca aattgagcca gtcagcgaga atgacccgcg aaatttaaac ggaaactgtc
     1801 cccgccaccg atgcgaggag tccattgtga taaatcgaca acgattgcag aattttctca
     1861 tcgagctgat caagatcgac agcttgtcgc ggcgcgaata cgacgtcgcc atgcgcctta
     1921 aacgggagat ggaagaactt ggcgcggcgg tttcgatcga tgacgccggc gcgaaggtcg
     1981 gcggcaacgt cggcaatttg ctcgccaagt ttcccggtaa cgcggcggcg agccagccgc
     2041 tgctgctgtc cgcgcatatg gacacagtgg tccccggcga aggcatcgta ccgatcctcg
     2101 aaggtgacat cttgcgcacc gacggtacca ccgtgctcgg cggcgacgac aaaagcggcg
     2161 tcgcgatcat ttgcgaagtc atgcgagtta tcaaggagaa cggcctgccc tgctccgccg
     2221 tcgacgtcgt cttcacgatt tgcgaagagg ccgggctgat cggcgccaag tgtttggata
     2281 tcacaagcct gcgcgcccgc accggcctgg tgctggacag cgactcggtc ggtttcctgt
     2341 ttacccgagc cccggcggcc aaccgcatgg agtttcgcgt ccacggcctg gaagctcatg
     2401 cgggggtttg ccccgagaaa ggcatcaacg cgatcaaagt cgccgccgag ggcattgcgc
     2461 agatgcagct cggccgcatc gatcacgaga ccacggcaaa catcggcgtg gtcgaaggcg
     2521 gcatggcggt caatatcgtg cccaatcaag tcgtcctcaa aggcgaggcg cgcagccatg
     2581 actcggacaa actcaaacgc cagaccgaac atatgcagcg ttgcctcgaa gaagccgccg
     2641 ccagacattg gttagagttt gacggcaagc gccatcaagc gcgggtcgaa gccaagatcg
     2701 aacgcgacta cgaccgcatg gatgtacctg agaacgctcc catcgtccag ttggttcacg
     2761 ccgccgccag aaacttgcat ctcgacgtga aaacccatgc caccggcggt ggctgcgacg
     2821 ccaatgtatt gaatcaaaaa gggctggtag tcgccaacct cggcaccggt atgcgcgaga
     2881 ttcatacggt caacgaatgg ctcgatctga aagacctcta tctgtcggcg cagatggtgc
     2941 tggaagtcgt caaactcaat gccaccggct cgttacgctt tgctggctaa agaatgaaaa
     3001 cggaacaaat cgacctgtta ctcaaacagg aacgcggtca attcctcgaa ttcatcagcg
     3061 ccttcgagta ccggcgcagc tccggacaga agaaacgcga ggaagagctt tcccgcgaac
     3121 tggtgcgggt cctgtcggcc atggccaatg ccgacggcgg cacggttttg gtcggcgtcg
     3181 aacccgacag gagcgtcacc ggcatccccc atgatgacga cgaactacaa accttgatac
     3241 aggcgccgca gaacctgttg cgcccccaac ttacgccagc ctgtgaaaag attcgcttgg
     3301 gcagccagca actgctcaag ttcgacgtcg cttcgagcat ggacattcat cgcatcgccg
     3361 gcggccgctc gttttatcgc atcgccgcgg aaacaccggc cctggcagcc gagcagattc
     3421 actcgctgaa agaagccaag cgcaatgttt tttacgagcg ccagcaaacc ttcaacgcca
     3481 catggttcga cctcgacacc gatctggtga accaattcgt caacaaactt catcccggtg
     3541 aggatcccca ggcggtgtta agccagacct atcatttgct cgacaactcg cgcgccatcc
     3601 cctgccccaa catggcggct ctgctgctgt tcgccaaaga tccgacacgc tggcatccac
     3661 gcgccggcat cgacttcgtc aagtatgacg gcaatgagcg gcagcatggt acgtcactca
     3721 acgtcgttaa acggatgcgc tttgaagcgc cgcttgtgcg cttgatcgac gaagcggtgg
     3781 gccggatcaa agaacaggtt cgcgggcgca ccatccttca cgacttattt tttcgcgaac
     3841 gcttggagta tccgaccttt gcctggcagg aagcgctggt caacgcggtg gcccaccgcg
     3901 attattcgct caccggcgcg tcggtggaag tatggatgtt cgacgatcac atctgcgtgc
     3961 gcagccccgg cttgcctccg gcgccggtga cgctcgaaca gttgcgcctg cagaaaagca
     4021 ttcatttctc gcgtaatcca ttgatcgtcc gcgccctcgc cgatctggga tttgcccgcg
     4081 acatgggcga aggcgtgccg cgcatctatc aagaaatgga aacttacggt cttcatccac
     4141 cggagtttgc cttcgaagga ttcgttttta ccgtgaccct gcgtaacgcg ccggtctacg
     4201 acgaggccac gctgcgctgg ctgagccagt tcgccaacaa tcaagtcaat ttgcgccaac
     4261 gccgcgtgct ggcttacgcc tattgccatg gcaagacctt ttcgaccacc gagtatgagc
     4321 gcatcgccga ggtcgaccgt gacaccgcct accgcgacat tcgcctgatg gtcaaaaacg
     4381 gcatcgtcgc gccgctcaaa ccgaaaagcc gcagctatcg catcgtcgaa aagctctaag
     4441 cgaatcagcc gatggacttc tctcaactca tgggtcgggc ctccggccat gccgaggcgc
     4501 gcatcattca gaccgccgtt gagctcacca tcttcgacgc ccttgaggct gcggatcttg
     4561 acacagccgc tgtggcacag aacttaaaac tagacgaaca agcaaccgag ttattgctca
     4621 acgcgctgag cgcgctcggc ttgctggaaa aacgtggcag gtcgttttca ttgaccgggg
     4681 ttagccggcg ccatctactg cgccgttcgc cgcagtacgt cggcggcatg attagtttcg
     4741 atgccatgtt atggcacgac tgggaaaaac ttcccgaagc gatccgtacc ggtttgccaa
     4801 cccgtccgcc caacatgtat cagtccgatg cgcgggaaac cgctgttttc atcaacgcca
     4861 tggactcttt ggtcaaagcc cggggtgacg cggaaatttt ggccacggtt cttgactggt
     4921 ccaaggtcga gtcgattctc gacgtcggct ccggacccgc cacgtatccg atcgccttat
     4981 gcgaaaattt ttcccaactg cgcgcgacca tcgtcgatct gccgggcaca ttggaaatca
     5041 ccgatcgctt cgtgagaggt gcgaatcttt cggcgcgtat cgaactgatc gccggcgatt
     5101 atcgcatcga tccgatcact ggcagctacg acgcagtctt tctatcgaac atcattcatg
     5161 gtgagaacta cggctgcaac actacgctga tgcgcaagtt ggcggccaat ctaaagcccg
     5221 gcgggcgctt gatcgttaaa gaccatattc tcgacgatag ccgctcccaa ccacatgtag
     5281 gcgcgatctt tagtttgctc atgctcctga ctacagacgg cggccgttgt tatagcctca
     5341 cagagattga atcttggatg cagcaggcgg gaatgaaccg gatcgaacat atcgaactgc
     5401 cggcaccgat gacttcgtcc ctggtcatcg cgtggaagta gtagtcctgc gccaatttta
     5461 tgttagactg gctttattaa gggcaaaggc aggaacgcga ccatcgacga gcaagaacta
     5521 aaagcccggc agcaagcggt catcgacaac ttcaaacccg cctgcatctg caacaaaatc
     5581 cgccgcggca cagttgccaa agcgat
//
LOCUS       DASQKS010000108         6966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_480057, whole genome shotgun sequence.
ACCESSION   DASQKS010000108 DASQKS010000000
VERSION     DASQKS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480057"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..759
                     /locus_tag="VE170_02845"
     CDS             409..759
                     /locus_tag="VE170_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14396.1"
                     /translation="MKIVRLYSGSDEKSHFEDIEMQFGGNQPMLSTDMRAATGAVFRS
                     APVGLFLERHPAPRRQYLVTLSGSWEIEASNGVKRVFKTGDVMLADDTTGEGHTSRVL
                     GGEPHVFMTVPLAD"
     gene            902..1834
                     /locus_tag="VE170_02850"
     CDS             902..1834
                     /locus_tag="VE170_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14397.1"
                     /translation="MLRIAVPDLVSNSYFPIIAAVEMGFFKEEGFDAKVDLLFPIPKT
                     FEALRDGALDFVVGSAHATLLAFPEWQGAKLLAACGQHTYWFLVIRSDLKPQRGDLTI
                     VKGLRIGAAPGVNLSLQRMLVEAGIDPDKNNVQIMPIPGAAGPNVSFGLSAAKALEDG
                     TLDGFWANGMGCEVAVRRGVGTMVLDVRRGDGPTAARHYTFSALVATDKTIKDTPAAG
                     RAAVRALMKAHKALKQDPERATAVGQKRFPASEAALIAELVRRDLPFYDPTIPEEKVA
                     SMNRFAQDIGLLKAPATYDQVVAGQYRSLWNEAV"
     gene            2057..2635
                     /locus_tag="VE170_02855"
     CDS             2057..2635
                     /locus_tag="VE170_02855"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ14398.1"
                     /translation="MPPDSITDAEYVQKLQHGQTDAFEALIRRHEKTIFNLVYRMLGD
                     YDEAAETAQEVFLSAYRAIGQFRGDANFSTWLYRIALNHATTRRKSMNTRHQRQVSID
                     STEPMSDSRPGPAESMEKKEIRETVQRALNSLSADDASVILLRDLQDVPYDEVARVLE
                     VPVGTVKSRLHRARQALKSELASYFYAGRKAV"
     gene            2797..3807
                     /locus_tag="VE170_02860"
     CDS             2797..3807
                     /locus_tag="VE170_02860"
                     /inference="COORDINATES: protein motif:HMM:NF021550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HYJ14399.1"
                     /translation="MSPPLGFAQRVMAHVRDSEPKPSLWQRLLAPLISRVPVQAAAMA
                     VIAVFAVVLYQKAPALKPNNEDDPALNFSAQMASKETPLSAPTALSPARQASETGGKS
                     VTPGKAPADVAKLNAGEKSPRALSAVPAPPQSLQTASAGPSNAIESAGEESRDVAPRR
                     PPLRVQEVNMARDSGLMFGDQYGFNYPIPSSFGVMRQTPARTVPLVMEQPIALGDRVA
                     DFEFIVRRRSPQRRDTIENLSSSKASQESTEPDGSPANPPHPATPSNGRARIESIAEI
                     RFYNVAPEHFDIFKKELAAEANIESEPRITAREVEAAKQGDRQLLVKVTILPSDRSPT
                     SR"
     gene            complement(3890..4378)
                     /locus_tag="VE170_02865"
     CDS             complement(3890..4378)
                     /locus_tag="VE170_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYJ14400.1"
                     /translation="MAIPERYNTGYNIGVGGAVVRDDKLLFVRRASRHGRGNWQLPGG
                     FIEPDETIEQAVVREVHEETGVTAEVEAVLGLRSRYDPDSGNGVYVILLLRAIVGEPC
                     PDGHETDHAEFFSLEQIVQLNPLPPVNWEIAQRVLAPDRRLLQPKPLVNLNGAKFTLF
                     VG"
     gene            complement(4407..5588)
                     /locus_tag="VE170_02870"
     CDS             complement(4407..5588)
                     /locus_tag="VE170_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14401.1"
                     /translation="MIIDCDTHLMPRDAFNGVGGELETIKPTLKFDDQELFFDVDFPG
                     YPTEMTGTSPLPAPGSGAMFRSLWDPIARMQDYDGRLGIEQHVVLPQFSGWWSYLIEP
                     ELACPMARSHNQSLLRLMTEYAGRIFGAALVPLQDVTAAIGEMEWARDHGFCSVVLDK
                     VFPVKAHPYGEPLGSHRELWPFFKRAEELAMPIYLHNVQHGHRMSNLPIFQRDGLYIF
                     APQEGQMSLVSLITSGLLDEFPQLQFIFTEAGTSFIKPLVQWLDRVLDRPPVDYADQE
                     DPLNTRGALTKVGERLRRARAIAPAQVFLEKNAKPASYYFKNNFYFTIETEEPELPEA
                     IEFLGAERFLFATDYPHDDPGGRMKFEDVRLLREHKKIAEDAKDLIRFKNAQRLFHLG
                     V"
     gene            complement(5746..6249)
                     /locus_tag="VE170_02875"
     CDS             complement(5746..6249)
                     /locus_tag="VE170_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14402.1"
                     /translation="MTKTNLSLFAAGVVSLLAFTTPDLASADARSRQRDLDRNRARGQ
                     SSQWNELHRDRAELRRDQTELERDRADLERLQRSGASRQQISRKREEIRRDLEELAQD
                     HREISENLETLSSSRDRGYGRDTNVWQRDRLGRNDSLGWGWGRRDDRGRWGRNDTNGW
                     GWRNGRN"
     gene            6476..>6966
                     /locus_tag="VE170_02880"
     CDS             6476..>6966
                     /locus_tag="VE170_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYJ14403.1"
                     /translation="MANNPAYDPTDPRYFDISDLRSEVERVFSLCADCRMCVKYCGSF
                     PKLFDAVDDYCVEGKYADVNTKNLKSADVDKVVDLCFQCKLCYIKCPYTPGDHEWAID
                     FPRLMTRAKAQGVKKNGVALVDKVLGNPDRVGKLGTSIAPLANWGNENPLNRQLMESV
                     VGI"
BASE COUNT         1645 a         2042 c         1893 g         1386 t
ORIGIN      
        1 tcagcggcaa ggccgagatc gtttcgctac ccgaagccct cgacacgctg gtctatttgc
       61 agcgccaagc ctatggcgag cacaagagct ggccggaatt tcaccagcgc atggcgcgcg
      121 agcagcgcgt gatcattcgc atcaccatcg aaaacgtcgg ccccaaggta cgcggctaac
      181 gattactaaa tattcctggc cacgtgttcg acgagtcatt gaacttggcc aaagagccaa
      241 aaaaaaatcg cgccgcgcca tggacaagtg acagatacct ctgtgataaa agagcgaatg
      301 ggaaacccga cattgccaag ccagtatccc taagctgaaa aatgacgccg acagccaaat
      361 cgcgggtgtg gaaggcacaa tccaattgat catcgaaccg gaggatccat gaagatcgtc
      421 agactctaca gtggcagcga cgagaaatct cattttgaag acattgaaat gcaattcggc
      481 ggtaaccaac cgatgctcag caccgacatg cgcgccgcca cgggcgcggt gtttcgctcc
      541 gctcccgtcg gactattctt ggaacgccat ccggcaccgc ggcggcaata cctcgttacc
      601 ctgtccggat cctgggaaat cgaagcgagc aacggcgtca agcgggtttt taaaaccggc
      661 gacgtcatgc ttgccgacga caccacgggg gaaggtcaca cctcccgcgt gctcggcggc
      721 gagccccatg tgttcatgac cgtgccgttg gcggactgaa taattgcaag agccgcgagc
      781 tcatgatccg tccttgaacc ttttaacctt gaacttttga acgaaaaaag ccgttcaagg
      841 gttcaaaagt tcaaggttca aggtcgatca agaaaagtcg tcagtccaac atggagatca
      901 tatgctgcgt atcgccgtac ccgatctagt atcgaattct tattttccga tcatcgctgc
      961 cgtcgaaatg ggttttttca aggaagaagg cttcgacgcg aaggttgatc tattatttcc
     1021 cattccaaaa acctttgaag ctctgcgcga tggcgcgctc gacttcgtcg tcggctcggc
     1081 ccacgcgaca ttgctcgcct tccccgagtg gcagggcgcc aagctgctgg cggcctgcgg
     1141 ccagcatact tactggtttc tggtcatccg tagcgacctc aaaccgcagc gcggcgattt
     1201 aactatagtc aaaggcttgc gcattggcgc ggcgccgggc gtcaatttga gcttacagcg
     1261 catgttggtc gaagccggca tcgatcccga taaaaataat gttcagatca tgcccattcc
     1321 cggcgccgca ggccccaacg tttcctttgg cctatcggca gctaaagcgc ttgaagacgg
     1381 cacactggac ggtttctggg ccaatggcat gggatgcgaa gtggccgtgc ggcgcggcgt
     1441 cggcaccatg gtcctcgatg tccgccgcgg cgacggccca acggcggcac gccattacac
     1501 gttttctgcg ctggtggcga ccgacaagac gattaaggac accccggcag caggccgtgc
     1561 cgcggtgcgc gcactgatga aagcccacaa ggcactcaag caagacccgg agcgcgccac
     1621 cgcggtgggc caaaagcgct ttccagcctc ggaagcagcg ctcatcgccg aactggtgcg
     1681 gcgcgatctg cccttttacg acccgacgat tccagaggaa aaggtcgcga gcatgaaccg
     1741 tttcgcccag gacatcggcc tgctcaaggc accggcaacc tatgaccagg tggttgccgg
     1801 ccaataccgc agcctttgga acgaagccgt ctaagatcgc ctgctcggca atcaatcatc
     1861 caatggctgc attggattag aggctcactg cgccaaggct caaaaacgct gcgcaattga
     1921 tattaagcag ctaacttgta acaatttagg gactttacct agcccgaaaa caccgataga
     1981 ctgcctataa gacccgccaa tttaaaatta tcaacggaac ttttcggtga ataccaagtc
     2041 taaaccctca gaaacgatgc cgccagactc gatcaccgac gccgagtatg ttcaaaaact
     2101 ccagcacggg cagaccgacg cgtttgaggc attgatccgg cgccatgaga agaccatttt
     2161 caacctcgtc tatcgcatgt taggcgacta cgatgaagcc gcagagacgg ctcaggaagt
     2221 gtttctttcg gcgtaccggg ccatcggcca gtttcgcggc gacgccaatt tttccacatg
     2281 gctctatcgc atcgcgctca accatgcgac gacgcgccgc aagagcatga acacccgcca
     2341 tcaacgccag gtctccattg atagcaccga acccatgagc gattctcgac ccggtccagc
     2401 ggaatccatg gaaaaaaaag agattcgtga aaccgtccaa agagcgttaa acagtttatc
     2461 tgccgacgac gccagcgtga tccttctgcg cgacctccaa gacgtgccct atgacgaagt
     2521 cgcccgggtg ctcgaagtgc cggtgggaac cgttaagtca cgcttgcatc gcgcccggca
     2581 agcgcttaaa tccgaattag catcctactt ctacgccgga aggaaggcag tatgaactgc
     2641 gaagaggtcg acaagcacct aacggaatat ctcgacgaaa gcctcgacct ggcgatgacc
     2701 acgcgcgtcg caacccatgt gatttcttgc gcgcactgtc gcgccggatc caacgaatta
     2761 gccgactgca tccaacaagt ggcaagcttg ccggcgatgt cgccgccgct cggcttcgcc
     2821 cagcgggtga tggctcatgt gcgcgacagc gaaccaaaac cgagcctgtg gcagcgactg
     2881 cttgcgccgc taatcagtcg ggttccggtc caggcggccg ccatggcggt gatcgcagtt
     2941 tttgctgtag ttctctatca aaaagcaccg gcgttaaaac caaataacga agatgatccg
     3001 gcattaaatt tttcggccca aatggcgagt aaagaaactc cgctatccgc gcccacagcc
     3061 ctaagcccgg cacgccaagc ttcagagact ggcgggaagt cagttactcc gggaaaagct
     3121 ccggccgatg tcgccaagct caacgccggg gaaaaatcgc cgcgcgcgtt atcagccgtt
     3181 ccggcgccgc cccagtcgct tcaaactgcg tcggcggggc cgtcaaacgc tatagaaagt
     3241 gccggggaag aaagtcgcga cgttgctcca aggcggccgc cgctgagggt acaagaagtg
     3301 aatatggcac gagactccgg gctaatgttc ggcgatcagt acggcttcaa ctacccaatc
     3361 ccaagttctt tcggcgtcat gcgccaaacg ccagcgcgaa ccgtaccact ggtgatggag
     3421 cagccgattg cgctcggcga tcgcgtcgcc gactttgagt tcatcgtgcg ccgccgctcg
     3481 ccccagcgcc gcgataccat cgaaaattta agcagctcca aagcttcaca agaatccacc
     3541 gaaccagacg gatccccggc caatccaccc cacccagcta caccatcgaa cgggcgggcg
     3601 agaatcgaat ccattgcgga aatacgattt tacaacgtcg cgcccgagca ctttgatatt
     3661 ttcaagaaag aactagccgc agaagcgaac attgaatctg aaccgagaat cacagcgagg
     3721 gaagtcgaag ccgccaagca aggcgaccgt cagttattag tcaaagtgac gattttgccg
     3781 tcagatcgaa gcccaacctc tcgttaacct atccagaatt atccggttgt tgcctgcaag
     3841 gcgagtgagc cctgcgtgat tacatcatgc tggccatcag cagcgggatt caaccgacga
     3901 acagcgtaaa cttagctccg ttcaagttga ccagcggttt cggctgcaac aggcgacggt
     3961 ctggggcaag gacgcgttgg gcgatctccc aattcaccgg cggcagcggg ttgagctgga
     4021 cgatctgttc cagtgaaaaa aattctgcat gatcggtctc atggccgtcg ggacagggct
     4081 caccgacgat cgcgcgcaaa agcagaatca cgtaaacgcc attgccgctg tcgggatcgt
     4141 aacggcttcg caagccgagc accgcttcga cttcagccgt cacccctgtt tcttcgtgaa
     4201 cttcacggac cacggcctgt tcgatggtct cgtcgggttc gataaagccg ccgggcagct
     4261 gccaatttcc ccggccatgg cgcgacgccc gacgcacgaa cagtagctta tcgtcacgca
     4321 cgacggcgcc gccgacaccg atgttgtagc cggtattgta acgttcagga attgccactg
     4381 ggataaagcc tttccagcga aacgatctaa acccctaggt gaaacagcct ctgggcattc
     4441 ttgaagcgaa tcaaatcttt cgcgtcctcg gcgatctttt tgtgctcgcg caaaagccga
     4501 acgtcttcga atttcattct gccgccggga tcgtcatgag gataatcggt ggcgaaaaga
     4561 aaccgctcgg cgccgagaaa ttcgatcgct tccggaagct ccggttcttc ggtttcgatg
     4621 gtaaaataaa aattgttctt aaaataatag ctggccggct tggcattttt ttcgagaaag
     4681 acttgtgccg gagcgatcgc ccgtgcccgc cgcagccgct cgccgacctt ggtcaaagca
     4741 ccacgggtat tgagcgggtc ttcctggtcg gcataatcga ccggcggacg gtcaagcact
     4801 cgatcaagcc attgcaccaa cggtttgatg aacgaagtgc cggcttcggt aaagatgaac
     4861 tgcagctggg gaaactcatc gagcaagccg ctggtgatca gcgacaccaa gctcatctgg
     4921 ccttcttggg gggcaaaaat atacaacccg tcgcgctgga agatcggtaa attgctcatc
     4981 cggtggccgt gctggacatt gtgcagatag atcggcatcg ccagctcttc agcgcgcttg
     5041 aagaacggcc aaagctcgcg gtggctaccg aggggctcac cgtaaggatg cgccttgacg
     5101 ggaaatactt tatcgaggac caccgagcaa aagccatggt cgcgagccca ttccatttcg
     5161 ccaatggctg cggtcacatc ttgcagcggc accagagccg cgccgaaaat cctgccggcg
     5221 tattccgtca tcaagcgcag cagagactgg ttgtgtgaac gcgccatagg gcaggccagc
     5281 tccggctcga tcaaataaga ccaccagccg gaaaactgcg gcagcacgac atgttgctcg
     5341 atacccaggc gaccgtcgta gtcctgcatg cgcgcgatcg gatcccaaag gctgcgaaac
     5401 atcgcgcctg agccgggtgc cggcagcggg ctcgtgcccg tcatttccgt tggataaccg
     5461 ggaaagtcga cgtcaaagaa gagttcttgg tcgtcgaact ttagcgtcgg ttttatggtt
     5521 tcaagttcac cgccaacgcc gttgaaggcg tcccgcggca taaggtgggt atcgcaatcg
     5581 atgatcaatt tggagcctcc gaagcatatg tatgcatcgg ttataaggct ctcccgcgcg
     5641 ctatgtcaat tagccacgat cttgagggcg gaccggccgg tccgccctca aggaaaaatt
     5701 gctttgctgc gcgcctgacg cggcgcttag ataggctaga agggatcagt ttcgaccgtt
     5761 gcgccagccc caaccgttgg tgtcattacg cccccaacgg ccgcggtcgt cccggcggcc
     5821 ccaaccccaa ccgagggaat cattacggcc caaacggtcg cgttgccaaa cgttagtatc
     5881 ccgaccatac ccgcggtccc gactcgagct caacgtctca agattttcgc tgatttcacg
     5941 atgatcttga gccagttctt caaggtcgcg gcgaatctct tcacgcttac gagagatttg
     6001 ctgacggctc gcgccgctgc gctggagacg ctccaaatca gcgcgatcgc gctcaagctc
     6061 ggtttggtcc cggcgcagtt cggcacggtc ccgatgtaac tcattccatt gagagctttg
     6121 acctcgtgcc ctattccggt ccaaatccct ttgccggctg cgcgcatcgg cgctcgctaa
     6181 atcgggggtc gtgaaagcca gcagggatac cacccccgcg gcaaataatg aaagattagt
     6241 ttttgtcatg ttgagatcct ccttggccat tagacgccgt ggctgcgcta acgttaacga
     6301 acggctgttg ccaccatcat acataaccgg caagattgcg ctattgtctc ggccagcaag
     6361 cgcggccaac agaggattga gaagctcggg aagaagatag cgctatagtt tatttctttt
     6421 gccatcgcgc tagggtaagg tcacagcaag acacgacgag taagggagtg atgcgatggc
     6481 aaacaatccg gcctatgacc ccacggatcc gcgatacttc gatatctcag atttgcggtc
     6541 cgaagttgaa cgcgtcttca gcctatgcgc cgattgtcgc atgtgcgtca agtattgtgg
     6601 cagcttcccg aaactgttcg acgccgtcga cgattactgc gtcgaaggca agtacgccga
     6661 cgtcaatacg aaaaatctca agagtgcaga cgtcgacaag gtcgtcgacc tctgtttcca
     6721 gtgcaagctc tgctatatca aatgccctta cacacccggc gaccatgaat gggccatcga
     6781 ctttcctcgt ttgatgacca gggccaaggc ccagggggtc aagaaaaatg gcgtagcgct
     6841 ggtcgacaaa gtgcttggca atccggatcg ggtcggcaaa ttgggcacca gcatcgcgcc
     6901 gctggccaac tggggcaatg aaaatccgct caatcgccaa ctcatggaaa gcgtcgtcgg
     6961 tatcca
//
LOCUS       DASQKS010000109         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2587504, whole genome shotgun sequence.
ACCESSION   DASQKS010000109 DASQKS010000000
VERSION     DASQKS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2587504"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1232
                     /locus_tag="VE170_02885"
     CDS             222..1232
                     /locus_tag="VE170_02885"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HYJ14404.1"
                     /translation="MSENATTMARFVKSYEIRRAYQGGVEYPPAISGVQGAIDIHCHA
                     DAAHQDALSVAKLASTNGMRGLLYKSIAGKNRAESVQKVRNGLQRWAEAEKVEPILCW
                     AGSNVANRSAAPSVAKVREQLDGGINAMWMPVAMHANTLSKIGGRKSWWDPNAGRGEL
                     TPAMSWEEALKVGHYALDDKGRLKPEIKDIIGLLKEREVPLFFGHATHPEIFAFAEEC
                     QRIGHSRAVVDHPFSPFVNLSIAQMTQLGQAGVNINFTYDEISPLLGVDPFDMYKAIR
                     SIGVEHVTLSSDCGEPLFPNSVEALRQMRAYMEAFGLNKAELKTVCCDNPAKILGLPL
                     LS"
     gene            complement(1274..>2123)
                     /locus_tag="VE170_02890"
     CDS             complement(1274..>2123)
                     /locus_tag="VE170_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14405.1"
                     /translation="PYVVANEKGFFDREGLKVQLITFRGTNLMLTALLAGELDYVTIL
                     PFLTGAAARGLPVKIVAAVTKSSSYVMISRPEIDCVKALRGKRLGINSFGSSADYAAY
                     AAVGRSGMDPNKDVTILPIGGGTSDRMAALVSGTVDATVVTSPAEYQAEKQGLRVLMS
                     ATEMGALVRIPITGAGVTLKKIDKDGDEIVRVLRALRLSTLYLLQNPEYTHGLYQRVM
                     RVDASLAERLFKLYRDQYNPELTLPDGIVDDLLAVGTFRLKEKPKAALNHQTVRDWSF
                     AEKAKR"
BASE COUNT          482 a          636 c          590 g          415 t
ORIGIN      
        1 gcctgcgcat aatcgtatcc aggcaatcgg atcgaacgcc gttggcgttt tgcggagttg
       61 tggttcatcg gcgtaatacc taatcgcaat gatccaattc cgcaacagta actctccgac
      121 cgtaggaggc cggtttgaaa cccgcccgcc gatttgacac gctggggcgc cggtgttacg
      181 cctcgacagt gaaattttga acatcaagaa agcgagaccc tatgagcgaa aacgccacga
      241 ctatggctcg attcgtcaag tcctacgaga tccgccgagc ctaccaaggc ggcgtggagt
      301 atccgccggc gatcagcggc gtgcaaggcg ccatcgacat tcactgccac gccgacgcgg
      361 cgcatcaaga tgcgctctcg gtggcaaaac tcgcatcgac gaacggcatg cgcggcctgc
      421 tctacaaatc catcgccggc aagaaccgcg ccgaatcggt gcaaaaggtc cgtaacggat
      481 tgcaacgctg ggccgaggcg gaaaaggtcg aaccgatcct ctgctgggcc ggctccaatg
      541 tcgccaaccg ctccgcagcg ccgtcggtgg caaaggtccg cgagcagctc gatggcggca
      601 tcaacgccat gtggatgccg gtagcgatgc atgccaatac gctaagcaag atcggtggac
      661 ggaaatcctg gtgggacccg aatgcggggc gcggcgagct aacgccggca atgagctggg
      721 aagaagcact taaagtcggc cactatgcgc tcgacgacaa gggccggctc aagccagaaa
      781 tcaaagacat catcggattg ttgaaggaac gcgaggtgcc gctgttcttc ggccatgcga
      841 cccacccgga aatttttgcg ttcgccgagg aatgccagcg gatcggccac agccgcgccg
      901 tcgtcgatca tccgtttagt cccttcgtca acctgagcat cgctcagatg acccagttgg
      961 gccaggccgg cgtcaacatc aactttacct acgacgaaat ttcaccactg ctcggcgtcg
     1021 atcccttcga catgtacaaa gcaattcgca gcatcggcgt cgagcatgtc accttgtcga
     1081 gcgactgcgg cgagccgctg ttccccaact cggtggaagc gctgcgccaa atgcgcgcct
     1141 acatggaagc gttcggattg aataaggcgg aactgaaaac cgtctgctgc gacaatccgg
     1201 cgaaaatttt aggactgccg ctgctttctt aacggcctct cgcatactcg gcggatattc
     1261 actatgaata gcgctatcgc ttggcctttt cagcgaaact ccagtcccgc acagtctggt
     1321 ggttcagtgc tgctttgggt ttctctttta agcgaaacgt gccgacagcg agcaagtcgt
     1381 cgacgatgcc gtcgggcaga gtcaactccg gattgtactg atcgcggtaa agcttgaaca
     1441 accgctcggc gagcgacgcg tcgacgcgca ttactctttg atacaagcca tgcgtgtatt
     1501 ccggattctg caatagatat agcgtcgaca gccgcaacgc gcgcagcaca cggacgatct
     1561 catcgccgtc tttatcaatt tttttcaagg tcacaccggc tccggtgatt ggaatacgca
     1621 ccaaagcgcc catctcggtt gccgacatga gtacgcgcaa accctgtttc tcggcctgat
     1681 actccgccgg actggtcaca accgtcgcgt cgacggtgcc gctgaccagc gccgccatgc
     1741 gatcggaagt accgccgccg atcggcaaaa tcgtcacatc tttgttggga tccatgccgc
     1801 tacgccccac cgcagcatat gcagcatagt cagccgagct gccgaaggaa ttgatcccaa
     1861 gcctcttgcc acgcaatgct ttgacacagt cgatctcggg ccgcgaaatc atgacgtagg
     1921 agctgctctt ggtaactgcg gcaacgatct tgaccggtag accacgcgcc gccgcgccgg
     1981 tcaaaaacgg caagatagtc acgtaatcca attcgccggc gagcaatgcc gtgagcatca
     2041 gattggtgcc gcgaaaagtg atcagctgga ccttgagtcc ctcgcgatcg aagaagcctt
     2101 tttcattggc aacgacatag ggc
//
LOCUS       DASQKS010000110         6379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3837969, whole genome shotgun sequence.
ACCESSION   DASQKS010000110 DASQKS010000000
VERSION     DASQKS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837969"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2473
                     /gene="acnA"
                     /locus_tag="VE170_02895"
     CDS             <1..2473
                     /gene="acnA"
                     /locus_tag="VE170_02895"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HYJ14406.1"
                     /translation="KVKSGKEIAFMPARVLMQDLTGVPAVVDLAAMREAMQRLGGDPK
                     KINPLAPVDLVIDHSVQVDYFGSVDAFQKNSEIEFERNVERYAFLRWGQMAFDNFRLI
                     PPDVGICHQVNLEYLAPVVFRAKKDGAHFAYPDTVVGTDSHTPMINGLGVVGWGVGGI
                     EAEAALLGQPIIMLIPEVIGFKLHGKLPAGATATDLVLTVVQMLRMKGVVEKFVEFYG
                     SGLSSLSLADRATIGNMGPEYGATVGYFPIDDETLRYLKLTARDPHLIKLVETYAKEQ
                     GMFRTDATPDPMFTDSLELDLASVVPSISGPRRPQDRIALTDSKKAFAEALPSLMKTK
                     NADKKVAVQSNGDKFELKHGSVVISAITSCTNTSNPSVLIGAGLVAKKAGEKGLTTKP
                     WVKTSLAPGSRVVTEYLKDSGLLPHLENLGFHIVGYGCTTCIGNSGPLPESVANGIQE
                     GDLVAAAVLSGNRNFEGRIHANVRANYLASPPLVVAYALAGSMDVDFYHDSLGNDRTG
                     QPVFLKDIWPTPQEVQDVMNKSVRQEMFKKEYSRATEGDDRWRDMPVPEGELFQWDMQ
                     STYVREAPYFEGMGKTPNPIKPITGARALVMVGDSVTTDHISPAGSIEKNGPAARYLS
                     NNDVLPKDYNQYGARRGNHEVMMRGTFANIRLKNMLAPGTEGGVSVHLPDKKPMSIYD
                     AAMQYQKEGTPLIVIAGKEYGSGSSRDWAAKGPRLLGIRAAIAESYERIHRSNLIGMG
                     IIPLVFKPGENLATHGLTGFEKFDILGIGNDLKLRQEVTVKATADDGKTTEFHVICRI
                     DTPAELEYYRHGGILEYVLRQLLAT"
     gene            2509..3426
                     /locus_tag="VE170_02900"
     CDS             2509..3426
                     /locus_tag="VE170_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ14407.1"
                     /translation="MITRREALATLGKTSLALFFAPSFLPSGAAAAEGNSMATPDFIG
                     KFTANNPGLMTLQGTNQYIVGKESGLVIDVALSADSNMDGIIEQAEAMGVKKIAKILL
                     THIHSDHTGGALALRKRCGAKLGIHRSRKGYLGGEDFQYDDNDLIDFGGGELHVLHTP
                     GHESGHCCFYESGDKVLFSGDNILGYGTAVIHPPDGNMSDYMRTLERLLGFEMSVILP
                     GHGPLIGKPEAKVREYIQHRLKREQQVIAALRADRNTIGDITETIYTEVSPALKRVAE
                     FSVQAHLEKLERDGKVKKESGRYLLRSDN"
     gene            complement(3630..4283)
                     /locus_tag="VE170_02905"
     CDS             complement(3630..4283)
                     /locus_tag="VE170_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYJ14408.1"
                     /translation="MIHRYLFFVALALANACLIPPSRVHIPAPPKTDTRVSQTGIASW
                     YGPGFHGKATASGEIYDQTDMTAAHQTLPLGTKVMVTNMENGRSAEVSINDRGPFAKD
                     RIIDLSHTAALSIDMVGPGTALVRIDVIDSPVPLRSIRTALDYTLQLGSFSRLENAQQ
                     LRDRIVNNFDGVTIAPLRSKDTTYYRVQLGTFSNRRAADDMAKQLSQAGYTVIVMEK"
     gene            4450..5196
                     /locus_tag="VE170_02910"
     CDS             4450..5196
                     /locus_tag="VE170_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ14409.1"
                     /translation="MSGHSKWSTIKHKKAAKDAKRGKIFTKLIKEITVAARMGGGDIN
                     ANPRLRTAVLTARGSSMPSDNIERAIKKGTGELEGVTYEEIQYEGYGPGGVAIIAQVL
                     TDNKNRTVSEIRRMFSKHGGNMGETGCVGWMFDKKGILTVDKSQIDEDRLMDIALDAG
                     AEDVRDEGEVFEVIAPPEDFEKVKERLDSEKIVIASAQVSLVPKNTVDVDAKNVEQVL
                     KLSEELEDHDDVQNVAANFHIPDELMDKVS"
     gene            5201..5770
                     /gene="ruvC"
                     /locus_tag="VE170_02915"
     CDS             5201..5770
                     /gene="ruvC"
                     /locus_tag="VE170_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYJ14410.1"
                     /translation="MTDPGNKPLPRIILGIDPGTMATGWGVVQVIGSALRHMGHGTIN
                     SSSAEPQASRLRRIYIELAKVVREFHPDGVSLEKVFFAQNVQSALKLGQARGVALLAA
                     AEQDVEVHEYSAVEIKSAVVGYGHATKQQVQMMIGSLLKVSGPVPADAADALAAAICH
                     LNRQAFATRVAEVIAKTPTPLRPRGIRGR"
     gene            5813..>6379
                     /gene="ruvA"
                     /locus_tag="VE170_02920"
     CDS             5813..>6379
                     /gene="ruvA"
                     /locus_tag="VE170_02920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYJ14411.1"
                     /translation="MIAQISGKLAQKQPGEVVIDVGGVGYLIFIPLNVFYRLPDLGAP
                     ISLQIHTHVREDALQLFGFHDLGEKQVFLLLTGVSGIGPRLALNILSGIPAEELVRAL
                     RNGDQLRLVSIPGVGKKTAERMIVELKDKFATFTPAAIDSTAKPEASSQKMHDAVSAL
                     INLGYKKPEIEKTVRDVLKNDALSLEDVL"
BASE COUNT         1415 a         1769 c         1924 g         1271 t
ORIGIN      
        1 caaggtgaaa tcgggaaagg aaatcgcctt catgccggcg cgggtgctga tgcaagacct
       61 caccggtgtt cccgccgtgg tcgatctcgc ggccatgcgc gaggcgatgc agcgactggg
      121 cggcgatccc aagaagatca atcccttggc gccggtggat ctggtcatcg atcactcggt
      181 gcaggtcgac tatttcggct ccgtcgatgc gtttcaaaag aactccgaaa tcgaattcga
      241 gcgcaacgtc gagcgctatg cgttcctgcg ctggggccag atggcgttcg acaattttcg
      301 tctgattcca cccgatgtcg gtatctgtca tcaggtcaat cttgaatatc tcgcgccggt
      361 ggtcttccgc gcgaaaaaag acggcgcgca cttcgcttat cccgacaccg tcgtcggcac
      421 cgattcgcac acaccgatga tcaacggtct cggtgtcgtc ggctggggcg tcggcggtat
      481 cgaggcggaa gccgcgcttc tcggtcagcc gatcatcatg ctgattcccg aggtcatcgg
      541 ctttaagctg cacggcaagc tgcctgccgg cgcgacggcc accgatctgg ttttgacggt
      601 ggtgcagatg ttgcgcatga agggcgtggt tgaaaagttc gtcgagttct acggctcggg
      661 actgtcgagt ttgagcctcg ccgatcgggc gaccatcggc aacatgggac cggagtacgg
      721 cgccaccgtc ggctatttcc ccatcgacga tgagactctg cgctacctga agctcaccgc
      781 acgtgatccg catctgatca aactggtcga aacctatgcc aaagagcagg gaatgttccg
      841 cactgatgcg acgcccgatc cgatgttcac cgattctctg gaattggatc ttgccagcgt
      901 ggtgccgagc atctccggtc cgcgccggcc gcaggatcgc atcgccctga cggacagcaa
      961 gaaagccttt gccgaagctt tgccttcgct gatgaaaacc aagaatgcgg acaagaaagt
     1021 tgccgtccag tcgaacggcg acaagttcga gctcaaacac ggctctgtgg tgatctcggc
     1081 gatcaccagc tgtaccaaca cttccaatcc atcggtattg atcggcgccg ggttggtggc
     1141 gaagaaagcc ggtgaaaagg gcctcacgac caagccctgg gtaaaaacca gcttggcgcc
     1201 gggctcgcgg gtggtcacgg aatacttgaa agacagcggg ctgctgccgc atttagaaaa
     1261 tctcggtttt catatcgtcg gctacggctg caccacgtgc atcggcaaca gcgggccatt
     1321 gccggagtct gtcgccaacg gtatccaaga gggcgatctg gttgccgccg cggtgctgtc
     1381 gggcaatcgt aacttcgagg gacggattca tgccaatgtg cgggcgaact atttagcctc
     1441 gccgccatta gtggttgcct acgctctagc cggcagcatg gacgtggatt tttaccacga
     1501 ttctctgggc aacgaccgaa ccggccagcc ggtatttctg aaagacatct ggccgacgcc
     1561 gcaggaagtt caggatgtga tgaacaaatc ggtgcgccag gaaatgttta aaaaggaata
     1621 cagccgggcg acagaaggcg atgaccgctg gcgcgacatg ccggtgcccg agggcgaatt
     1681 gttccagtgg gacatgcagt cgacctatgt gcgtgaagcg ccgtacttcg aaggcatggg
     1741 caagacgccc aatccgatca aaccgatcac tggcgcgcgg gcgttggtga tggtcggtga
     1801 ttcggtgacc accgatcata tttcaccggc cggttcgatt gagaagaacg gcccggcggc
     1861 gcgctatctg agcaacaacg acgtgctgcc caaagattac aatcaatacg gcgcgcggcg
     1921 cggcaatcat gaagtgatga tgcgcggcac ctttgccaat atccggttga agaatatgtt
     1981 ggctccgggc accgagggcg gcgtgtcggt gcatcttccc gacaagaagc cgatgtcgat
     2041 ctacgacgcg gcgatgcaat atcagaaaga aggcacgccg ctaatcgtca tcgccggcaa
     2101 ggaatacggc tccggctcgt cgcgcgactg ggccgctaag gggccacgct tgctgggcat
     2161 ccgcgccgcc atcgccgaga gctacgagcg catccatcgc agcaacttga tcggcatggg
     2221 catcatcccg ttggttttca agccggggga aaacttagcg acccatggac ttaccggttt
     2281 cgaaaaattc gatatcctcg gcatcggcaa cgatttgaaa ttacgccagg aggtgacggt
     2341 caaagcgacc gcggacgacg gcaagacgac cgagttccac gtcatctgcc gcatcgatac
     2401 gccggcggaa ttggaatact atcgccatgg cggcattcta gaatacgtgc tgcggcagtt
     2461 gctggcgact tgaaaaacag cactccgccg cgtagtgcaa gaaaatttgt gatcactcga
     2521 agagaagcgc tggcgacgtt agggaaaacc tcgctggcgc ttttttttgc cccgtcgttt
     2581 ttgcccagcg gcgccgccgc cgcggaagga aattctatgg ctacgcctga cttcataggc
     2641 aaattcaccg ccaacaatcc cggtcttatg accttgcaag ggaccaatca atatatcgtc
     2701 ggcaaggagt cgggcttggt catcgacgtc gcgctcagcg ccgattcgaa catggacggc
     2761 atcatcgagc aggccgaggc catgggcgta aaaaaaatcg ccaagatttt gctcacgcat
     2821 attcatagcg atcacaccgg cggcgccctg gcgctgcgca aacgttgcgg cgccaaactc
     2881 ggcatccatc gctcgcgcaa aggctatctc ggcggcgaag attttcaata cgacgacaac
     2941 gacctgatcg attttggcgg cggtgaactg catgtgctgc atacgccggg ccacgagtcg
     3001 ggtcattgtt gcttttatga aagcggcgat aaggttttgt tcagcggtga taatatcctc
     3061 ggctacggca cggcggtgat tcatccgccg gacggcaaca tgagcgatta catgcgcacc
     3121 ctcgagcgat tgctgggctt cgagatgagc gtgatcctgc ccggtcacgg accgctgatc
     3181 ggcaaaccgg aagccaaggt gcgagaatac atccaacacc gtttgaagcg tgagcagcag
     3241 gtaatcgcgg cattgcgcgc cgatcgcaat actatcggcg acattaccga gacgatttac
     3301 accgaggtct cgccggcgct caagcgggtc gccgagttct ccgtgcaggc gcatctggaa
     3361 aagctcgagc gcgatggcaa ggtcaaaaag gaatccggcc gttatctttt gcgcagcgac
     3421 aattgagctg ggccaccggc ggagtcgaaa cgctaaccct tcggcacgta ttaatcgtaa
     3481 atcctgtcat gccgcggcag cggcggccaa ccgatcgggg ttattgatga gagcgaagca
     3541 tgagttgtct tgctgcgcga gctcgaagcc gtcgcgatcc tgggcatcgg gattaagcca
     3601 tcgtcgcctt gggcgggcgg cgtcatttac tacttttcca tgacgatcac cgtgtagccg
     3661 gcttggctca attgcttggc catgtcgtca gccgccctgc ggttggagaa ggtgccgagt
     3721 tgcacccgat agtaagtggt gtctttcgat cgcaacggcg cgatcgtgac gccgtcgaag
     3781 ttgttgacga tgcggtcgcg cagctgttgg gcattttcca accgagagaa cgaacccaat
     3841 tgcagcgtgt aatcgagcgc tgtgcggatc gagcgtagcg gcaccgggct gtcgataacg
     3901 tcgatccgta ccagcgcggt gccgggtccg accatgtcga tggatagggc ggcggtgtga
     3961 gagaggtcga taatccggtc tttagcaaac gggccgcggt cgttgatcga cacttcagcg
     4021 ctgcggccgt tttccatgtt ggtcaccatc actttggttc ccagcggcaa ggtctggtgc
     4081 gccgcggtca tgtcggtttg atcgtaaatc tcgccgctgg cggtggcctt gccgtgaaaa
     4141 ccggggccgt accaagacgc gatgccggtt tggctaaccc gcgtgtcggt ttttggtggc
     4201 gccggaatgt ggaccctgga cggcggtatc aagcaggcgt tggccagagc taaggcgaca
     4261 aaaaataaat aacggtggat catgccatgg agttcttagc gggcgattat attgcaaaga
     4321 ccagtaaaaa aaaataaccc gacgcggatc tagccggttc attgcgcccc ctcgtgcagt
     4381 cttgatcgag tttacccggt ctgggctttg tgttagcttg tcggcaactt accagggagc
     4441 gattgaagta tgtctggcca ttccaaatgg agcacgatca agcataagaa agccgccaag
     4501 gatgccaaac gcggcaaaat atttaccaag ctgattaagg aaatcaccgt ggcggcgcgc
     4561 atgggtggcg gcgatatcaa tgccaatccc cggctgcgca cggcggttct caccgcgcgc
     4621 ggcagcagca tgccgagcga taatatcgag cgcgcgatca aaaaaggcac cggcgaactc
     4681 gaaggggtaa cctacgaaga gattcaatac gaaggctacg gtccaggcgg cgtggcgatc
     4741 atcgcccagg tgttgaccga caacaagaat cgtacggtgt cggaaattcg ccggatgttt
     4801 tccaagcatg gcggtaacat gggggaaacc ggctgcgtcg gctggatgtt cgacaagaag
     4861 ggcatcctca ccgtcgacaa gtcgcagatc gacgaggacc gtttgatgga tatcgcgctc
     4921 gatgccggcg ccgaggatgt ccgcgacgag ggcgaggtct tcgaagtgat cgccccgccg
     4981 gaggatttcg aaaaagtcaa agaacggctg gacagcgaaa agatcgtcat cgccagcgct
     5041 caggttagcc tggtgcccaa gaataccgtc gacgtcgacg ccaaaaatgt cgagcaagtc
     5101 ttaaaactga gcgaagaact ggaagatcat gacgacgtgc aaaatgtcgc cgctaatttt
     5161 catattcccg atgagctgat ggacaaggtg agctaaacgc gtgaccgacc ctggcaacaa
     5221 accactgccg cgcatcatcc tgggtatcga cccgggcacc atggcgacgg gctggggcgt
     5281 cgtccaggtt atcggcagcg ccttgcgcca tatgggtcat ggcacaataa attcgtctag
     5341 cgccgagccc caagcgagcc gcttgcggcg gatctatatc gaactggcga aggtcgtgcg
     5401 cgagtttcac cccgacggcg tcagcctgga aaaagttttc tttgcgcaaa atgtccaaag
     5461 cgccttgaaa ctcggccaag cgcgtggcgt cgcactcctg gcggcggctg agcaagatgt
     5521 cgaagttcac gaatatagcg cggtggagat caagtcggcg gtggtgggct atggccacgc
     5581 caccaagcag caagtgcaga tgatgatcgg atcgttatta aaagtatccg gaccggtgcc
     5641 ggcggatgcc gcggatgctc ttgccgccgc gatctgccat cttaaccggc aagcattcgc
     5701 cacccgggtt gccgaggtga tcgccaaaac gccgacgccg ttgcggccgc gcggtatccg
     5761 ggggcgttga acggccaatg gaccattcgt taaccctgcc cgtgggctcg acgtgatcgc
     5821 gcaaatcagc ggcaaactcg cgcaaaaaca gcccggcgaa gtggtcatcg acgttggcgg
     5881 cgtcggctat ctcattttta ttccgctcaa tgttttttac cgcttgcccg acctcggcgc
     5941 gccaataagc ttgcagattc atacccatgt gcgcgaagac gcgctgcagt tgttcggctt
     6001 tcacgatctc ggcgagaagc aggtgtttct cctgctcacc ggggtgtcgg gtatcggccc
     6061 cagattagcg ctcaatattc tatcgggcat tcccgcagag gaattggtcc gggcgttacg
     6121 caatggcgac cagctgcgct tggtatcgat cccgggggtg ggtaagaaaa cggccgagcg
     6181 catgattgtc gaattgaaag ataaattcgc gacgttcacg ccggccgcca tcgacagcac
     6241 ggctaaaccc gaagcatcgt cgcaaaaaat gcacgacgcg gtatccgcct tgatcaacct
     6301 gggatataaa aaacccgaga tcgaaaaaac cgtccgtgac gtgctcaaga acgacgcgct
     6361 ctcactggaa gacgtcctg
//
LOCUS       DASQKS010000111         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_480578, whole genome shotgun sequence.
ACCESSION   DASQKS010000111 DASQKS010000000
VERSION     DASQKS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480578"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VE170_02925"
     CDS             <1..610
                     /locus_tag="VE170_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ14412.1"
                     /translation="EFNIPDDGRIMLFVGTGFRRKGLDRLLRLWRHDAPPHCYLLVVG
                     NDAHLASYRKVWRGNKQVVFAGARPNVEEFYAAADLLILPSVQEAFGNVVLEALASGL
                     PVLTVAGVGALDKAGEKLREGILDNPDDIAEIRAKILRLSNPLRWPSLSRQARAVAEK
                     YSWDAYLDQVEALLFDFVKLSPQAAHTTSAVMPPLSATQKMP"
     gene            617..1525
                     /locus_tag="VE170_02930"
     CDS             617..1525
                     /locus_tag="VE170_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ14413.1"
                     /translation="MTPQVSILIPTQGRMRELRELLSSLAQMDERDRIPHEIIVANNA
                     PDELTAQMVEKLVGEYGAHEPGRWRHVREPLPGKSLALNKIIPLAQAEILGFIDDDVT
                     VEKAWLRVTAAFFSQYPYDVKQGSILVPSQFSGDANLHNLLNRYRTICYYQKPGAEVR
                     EIRSLNGANMALRREFLQRCGLFDERIGPGRSGTSMDVEFGERVMRVGGRIGYAPSSI
                     VYHQVDWDRLTESYFRRRHEMQGRSRLIYKQCSALSIAGNLLRSLLEFGVYSVAHNER
                     RKYRAKGRIYHYRAMANDKIRSRSAV"
     gene            1592..2626
                     /locus_tag="VE170_02935"
     CDS             1592..2626
                     /locus_tag="VE170_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ14414.1"
                     /translation="MSETYIKTEIEAVQESCDVRIVSTKPANLPAKNHAPYEQLTDLA
                     AIREVIEEFQPDVLHSHWLHSVKILGKLSRLTGVPFTVRAHSFDSIWPEHYSVVQRVP
                     FFGAFRNPSHIRRALDLINSELCLGVLNFPFTRSRLERAGIRPDKLFDCYPVVNYRLF
                     HNEEPNGDAIMNVGACIPKKKMEDFLDLALAAPKLRFDLYALGYRIDELRETAQSRKS
                     PVHFNPPLELEEMPAAYKQHRWLIYTAAPQGNVGWPLSIAEAQASGVGVCMANIRGDL
                     RDYVGEAGFLYDTLDEAIKITSQPFSEELRQLGFAHAKKSDAFEHRALLFDLWAKARS
                     KVGGSVSQAN"
     gene            complement(2628..3701)
                     /locus_tag="VE170_02940"
     CDS             complement(2628..3701)
                     /locus_tag="VE170_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14415.1"
                     /translation="MQKALVTLLISIPFLTLVLATPPSAAQETSGQILAKLEKLSAEN
                     RHKVIVERARSEKEVTFYSSLQTVDADAYVKAFNKRYPFIKVNIYRISGQKQVLMIQT
                     EFNAGKHGFDITNASAAQAFGIKKTGALDPYQSPQRQYFSPAHKDKEGFFTPTYIVPV
                     VLGYNTNMVKRGGAPKRYEDLLESKWKANMILDIEEFPWFAVLLKHYGREKGLEYMRR
                     LARQEILMGRGRTTQAQLISAGERAIGIALNSSSVLEFKTRGAPIDWTLLDPYYAKPN
                     MLMLARHAPHPHAAALLIDWTLSEEGQSLLASLGYVVARKGVKQSVPALLEKESFLAD
                     PEFIGPILEETGRDFRSVFTGGR"
     gene            complement(3945..4322)
                     /locus_tag="VE170_02945"
     CDS             complement(3945..4322)
                     /locus_tag="VE170_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HYJ14416.1"
                     /translation="MSAKKPRSGNGELENILSEARQLRARRGVYRDRALKILPWICAW
                     CGRDFTSKKLHELTVHHKDRDHNNNPPDGSNWELLCMYCHDSEHSRSLDREASSDTAV
                     HDDEDSPATYNPFAVLKELKPKK"
BASE COUNT          900 a         1251 c         1258 g         1004 t
ORIGIN      
        1 ggagttcaac attccagacg acggtcggat catgctgttc gtcggcaccg gttttcggcg
       61 caagggtcta gatcgcctac tgcgtttgtg gcgtcacgat gcgccgccgc attgctacct
      121 gttggtagtc ggcaacgacg cccatttggc ctcctatcga aaagtctggc gcggcaataa
      181 gcaagtcgta tttgccggcg ccaggccgaa tgtggaagaa ttctacgccg ccgcggatct
      241 cctgatattg ccctcggtgc aagaagcttt cggcaacgtc gtattggaag ctttggcctc
      301 cggcttgccg gtgctgaccg tggccggggt cggcgccctc gataaagccg gcgaaaaatt
      361 gcgcgaagga attcttgata accccgatga catcgcggaa atcagggcaa aaatcttgcg
      421 cctgtcgaat ccgttgcgct ggccctcgct gtcgcgccag gcacgcgccg tggcggagaa
      481 gtacagctgg gacgcttatc tggaccaggt tgaagcgctg cttttcgatt tcgtcaagct
      541 ttcgccgcaa gccgcccaca ccacgtccgc ggtcatgccg ccattatcgg cgactcagaa
      601 aatgccctga gtcgccatga cgccgcaagt cagcattctg attcccaccc agggccgtat
      661 gcgcgagttg cgcgagctcc tgtcgagctt agcgcaaatg gacgagcgcg accgtatccc
      721 ccacgaaatc atcgtcgcca ataatgctcc ggatgagctt acggcccaaa tggtcgagaa
      781 gcttgtcggc gagtacggcg cccatgaacc cggccggtgg cgccatgtgc gcgaaccgct
      841 gccggggaaa tctctcgcat tgaacaagat cattcccctt gctcaggccg agatactcgg
      901 gtttatcgac gacgacgtta ccgtggaaaa ggcctggctg cgggttaccg cggcgttctt
      961 cagccaatac ccctatgacg tcaaacaggg atcgatactg gtgccatcgc agtttagcgg
     1021 cgacgcaaac ctccacaact tactcaatcg ttatcggacg atttgttatt atcaaaaacc
     1081 cggtgcggag gtcagagaga tccgcagcct gaacggcgcg aatatggcgt tgcggcgcga
     1141 gttcctgcaa cgctgcggcc tttttgacga acggatcggc ccggggcgat cgggcacgtc
     1201 aatggacgtc gagtttggcg aaagagtgat gcgtgtcggc ggtcgtatag gttatgcgcc
     1261 gagctcgatc gtctatcatc aggtcgattg ggaccgactc accgaaagtt attttcgccg
     1321 gcgccacgaa atgcaggggc gcagccggct tatttacaag caatgcagcg cgttgagcat
     1381 cgccggcaac ctgctgcgtt cgctgctcga attcggcgtc tactcggtgg cgcataacga
     1441 acgaagaaaa tatcgtgcta aaggccgcat ctatcattat cgcgccatgg ccaatgacaa
     1501 aatccgcagt cgatctgccg tttaattcgc gcttcaccgc attgccatga aaccttgccg
     1561 ggtgctttac gtgctaaaaa atttcccgca gatgtcggaa acttatatca agaccgagat
     1621 cgaagcggtg caggaatcct gtgatgtcag gattgtctcg accaaaccgg cgaatttgcc
     1681 ggcaaaaaat catgcaccct acgagcagtt gacggatctg gcggcgattc gcgaagtgat
     1741 tgaagagttc cagcccgatg tcctgcacag ccactggctc cattcggtta agatcctcgg
     1801 taaattgtcg cgcctgaccg gggtgccgtt caccgtgcgc gcccactcct tcgactcgat
     1861 ttggccggag cattactcgg tggtgcaacg cgtgcccttt ttcggcgctt ttcgcaatcc
     1921 gtcccatatt cgccgcgccc tggatttgat taacagtgaa ctttgtctcg gggttttgaa
     1981 ctttcccttc acccgttccc ggctcgagcg cgcgggcatt cgtccggaca agctcttcga
     2041 ctgttatcct gtggtgaatt atcggctgtt ccataacgaa gagcccaacg gcgatgcgat
     2101 catgaacgtc ggcgcctgca tcccaaaaaa aaaaatggaa gactttctcg atcttgcctt
     2161 ggccgcgccg aagctgcgct ttgatctgta cgccctcggc tatcggatcg acgagttgcg
     2221 cgagactgcc caaagccgca agagtccggt gcatttcaac ccgcccttgg agcttgaaga
     2281 gatgcctgcg gcctacaaac agcatcgctg gctgatctat accgcggcgc cccagggcaa
     2341 tgtcggttgg cccctgtcca tcgccgaagc gcaggcctcc ggtgttgggg tatgcatggc
     2401 gaatatccgg ggcgacctgc gcgattatgt cggcgaagca gggtttctct acgacacgtt
     2461 ggacgaagcg atcaaaatta cctcgcagcc tttttcagaa gagttgcgtc agctcggttt
     2521 tgcccacgcg aagaagtccg acgcattcga gcatcgtgcc ttgctgttcg atctctgggc
     2581 aaaggctcgg tcaaaggttg gcgggtccgt ctcacaagct aattgattta gcgcccgccg
     2641 gtgaacacgc tgcgaaaatc tctgccggtt tcttccagga tcggcccgat gaattccggg
     2701 tcggcgagaa aactctcctt ttccaacagc gccgggacgc tttgtttcac gcccttgcgc
     2761 gcgaccacgt aacctaagct cgccagcagc gattgtccct cctccgacag cgtccagtcg
     2821 atcaacaacg ccgcggcatg gggatggggc gcgtggcgcg ccagcatgag catgttgggt
     2881 ttggcgtagt acggatcgag cagggtccag tcgatcggcg cgccgcgagt tttgaactca
     2941 agcaccgaag agctgttgag cgcgatgccg atggcgcgct cgccggcgct gatcagttgt
     3001 gcctgggtag tgcggccgcg tcccatcagg atctcctggc gggcaagacg cctcatgtac
     3061 tccagtcctt tttcccggcc ataatgtttg agcaggacgg caaaccaggg aaactcctcg
     3121 atgtccaaaa tcatgttggc tttccacttg gactcgagca gatcttcata gcgcttcggc
     3181 gcgccaccgc gcttgaccat gttggtgttg tagccgagca ccacgggaac gatataggtc
     3241 ggcgtgaaga agccttcttt gtctttatgg gcgggcgaga aatactgtcg ctgcgggctc
     3301 tggtacggat ccaacgcgcc ggtttttttg atgccgaaag cttgcgccgc cgaggcattg
     3361 gtgatgtcaa agccatgctt gccggcgttg aattcggtct ggatcatcag cacctgcttc
     3421 tgccccgaga tgcggtagat gttgaccttg atgaacggat agcgcttgtt gaacgccttg
     3481 acgtaggcgt ccgcgtcgac ggtctgaagc gagctgtaaa aggtcacttc tttctcgctc
     3541 ctggcgcgct cgacgataac cttatggcgg ttttccgccg agagcttttc aagcttcgcg
     3601 agaatttgac cggaggtctc ctgcgcggcg cttggcggcg ttgccagcac caatgtgaga
     3661 aacggtattg aaatcagcaa agtgacaagc gctttttgca taaccatcgc ctcaacgatc
     3721 tgccaagaag tgaacgcgaa tgacgaggat taaatcgacc ctcgccgccg ggaccagcga
     3781 atcattcacc ggtcgaagaa gattttttat cggcctccgc ataaagactc ccgtccttta
     3841 gcacaaaatc aacggtaatg tcgcccgccg ggccgggact gacagcgaat tcaacggttg
     3901 ccaatttgca gtcggtgcac tctgctcgcg gcagtaacta cgggttattt tttcggtttc
     3961 agttctttta ggacggcgaa tggattgtag gttgccgggc tgtcttcatc gtcgtgcacg
     4021 gcggtgtcgc tgcttgcctc tcgatcgagg gaccgtgagt gctcgctgtc gtggcagtac
     4081 atgcacagca gttcccagtt gctgccgtcg ggcggattat tattatggtc gcggtctttg
     4141 tggtgcaccg tgagctcgtg cagtttctta ctggtaaaat cccggccgca ccaggcgcat
     4201 atccacggca agatcttgag cgctcgatcg cggtaaacgc cccggcgcgc gcgcaactgc
     4261 cgcgcctcgg agagaatgtt ctccagctcg ccgttacccg atcggggttt ctttgctgac
     4321 attactggaa gtttatgact gctcacgact tacagcaaga ctgttcgaag ttattagtga
     4381 aaccccggaa ataatatccg ataagatatt atc
//
LOCUS       DASQKS010000112         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2998474, whole genome shotgun sequence.
ACCESSION   DASQKS010000112 DASQKS010000000
VERSION     DASQKS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998474"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VE170_02950"
     CDS             <1..1035
                     /locus_tag="VE170_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14417.1"
                     /translation="ARLCRIVGESQRGLVQFTHGAFATPEHVAHISEWYDTILQETRR
                     PLIGESIRHRWSEPELWRKQLHDVEARARQGYAAYAMTSTRRSVRRWTLKDGARLDII
                     PAWKKLSGLPFAERMERLRDTAVRAALAKETVDGKPIDFSRRWDLIGVEKVARRENHS
                     LQGKSVAELADAQHKAPIDAMLDLALAEDLETVFEDSTTQGDEQAVKEIFCNPNVLLG
                     QSDAGAHVASGNPGFGYATLMLAYWVRERKLMTLEDAIKKLTFLPASIFGIHDRGLLR
                     RGLAADLFVFDPDRIDLAPPAKVNDLPEGAPRFVQGAKGIHSTIVNGKVLMDKGAHTG
                     VYPGRVLRSV"
     gene            complement(1090..2091)
                     /locus_tag="VE170_02955"
     CDS             complement(1090..2091)
                     /locus_tag="VE170_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14418.1"
                     /translation="MKAGKFLAAVTLFMCGAISVHAQTIDEVYKRALTEGGTLNFYAT
                     LAQINAEIILPAFEKRFPGIKVNHVDATADQLATRIIAESRGGRIVADVFQTLLDGVM
                     RIQGQGLFLERLYPEATAYPDELKGTTWLATDLIYIVPAWNTSKVKKDEEPKQFEDFA
                     DPRWKNRLIAEPRDFQLLLGLAKHKYKNDDKAVALLKRIAANNPEFHKGHSQLAELLV
                     AGQAAACLTCYSHHYPVRKRKGAPVDYMLTEGTGDIDATAIMKTAPHPNTAQLFARWS
                     ASEEGQKIYAAGGRLPAHPKVEPTEKTRPAKVYAVGEDDVKDYPKYEKMWKEIFGLR"
     gene            complement(2338..>2964)
                     /locus_tag="VE170_02960"
     CDS             complement(2338..>2964)
                     /locus_tag="VE170_02960"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14419.1"
                     /translation="GEAPGYLGPQRYVRCVEYEVRAINYRKGAMYQSVTTPEGDKPWM
                     DLPREGAYLRRCREAVPGVTAVCKLGRQANYNIFIAMKKMSEGDPGRAAAAALTFDHA
                     KNVFVFDDDIDVFNPTDVLWAIATRVQPHLQISITPPIFRGNMLDPSLDDEIKTSCMI
                     VDATKPLDRPFSPVSKCPDEAMARIKLADYVPGEVLQHIPMDRTTYWA"
BASE COUNT          602 a          901 c          841 g          620 t
ORIGIN      
        1 gcccggctgt gccggatcgt cggcgagtcc cagcgcggct tggtgcaatt cacccacggc
       61 gccttcgcca cgcccgaaca tgtcgcgcat atcagtgaat ggtacgacac gattttacag
      121 gaaacccgcc ggccgttaat cggtgaaagc attcgccacc gttggagcga accggagcta
      181 tggcgcaaac agctgcacga cgtcgaagcg cgtgcccgcc agggctacgc cgcctacgcc
      241 atgaccagca cgcgccgatc cgtgcgccgc tggacgctca aagacggcgc tcgtctcgat
      301 atcattccgg cttggaagaa actttcgggg ttgccgttcg ccgaacgaat ggagagactg
      361 cgcgacaccg ccgtgcgcgc ggcactggcg aaagaaaccg tcgacggaaa accgatcgac
      421 ttctcgcgcc gctgggatct gatcggtgtc gagaaagtag cgcgccggga aaatcacagt
      481 ctccaaggca agagcgtcgc cgagctggcc gacgcccagc acaaagcgcc gatcgacgcc
      541 atgctcgacc tcgccctggc ggaagatttg gaaaccgttt tcgaagactc gacgacccaa
      601 ggcgacgagc aagcggtcaa ggaaatcttc tgtaacccca acgtcctgct tgggcagtcc
      661 gacgccggcg cccatgtcgc cagcggcaat cccggttttg gctacgcgac gttaatgctc
      721 gcctattggg tgcgcgagcg aaagctcatg accctcgaag acgccatcaa gaaactcacg
      781 ttcttgccgg cgtcgatctt tggcattcac gaccgcggcc tgctgcgccg cggcctggcc
      841 gccgacttgt ttgtcttcga tcccgacagg atagacttag ccccgccggc aaaagtcaac
      901 gatctccccg aaggcgcgcc gcgcttcgtc caaggggcaa aaggaattca ctccacgatt
      961 gttaacggca aagtgctcat ggataaaggc gcccacaccg gcgtttatcc gggaagagtg
     1021 ctgcgaagcg tgtaaagccg ggaatccccc catcccttcg gtaggagatt ctcgactatt
     1081 ggcttcagct cagcgcagcc caaaaatctc tttccacatc ttttcgtact tcgggtaatc
     1141 tttgacgtcg tcttcgccaa ccgcgtaaac tttcgccggc cgggtttttt ccgtcggttc
     1201 gactttcgga tgggccggca atctgccgcc ggcagcgtaa attttctggc cttcttcgct
     1261 cgccgaccag cgggcgaaca actgcgccgt attgggatgc ggcgcagttt tcatgatcgc
     1321 cgtggcgtcg atgtccccgg tgccttcggt caacatgtag tccaccggcg cgcctttgcg
     1381 tttacgcacc ggataatggt gtgaatagca ggtcaaacag gcggcggcct gtccggcgac
     1441 caacagctcg gccaactgcg aatgaccctt gtgaaattcc ggattgttgg cggcgatgcg
     1501 cttgagcagc gccacggctt tgtcgtcgtt tttgtatttg tgcttggcca ggccgagcag
     1561 caattggaaa tccctcggct cggcgatcaa gcgatttttc caacgcggat cggcgaaatc
     1621 ttcgaactgc ttaggctctt cgtctttttt taccttcgag gtattccacg ccggcacgat
     1681 ataaatcaag tcggtggcaa gccatgtcgt gcctttgagt tcgtccggat aggcagtcgc
     1741 ctccgggtag agtcgttcga gaaacaaccc ttggccctgg atccgcatga cgccatccag
     1801 caaggtttga aacacgtcgg cgactatcct tccgccgcgc gactcggcga tgatccgcgt
     1861 cgccagctgg tccgccgtcg cgtccacatg attcaccttg atgccgggaa aacgcttctc
     1921 gaatgccggc aagatgattt cggcattgat ctgcgccaag gtggcgtaga aattgagcgt
     1981 gccgccttcg gtgagcgcgc gtttataaac ttcgtcgata gtttgcgcgt gaacggagat
     2041 cgcgccgcac atgaacagcg ttacggctgc gagaaatttt ccggctttca tcacgatcct
     2101 cctgttaact catcccgtgt tagattgaca gtggccgcac tccatgagag ctgacttata
     2161 aagccgctgt agcaggtgca gtcatcgatc gtcaagctgg caggatccct ggctatcact
     2221 cgcgtacacc atggactcgt tcaaaagttc aaggttcaga ggttcaatcg gaggactgtg
     2281 tgtgcctccg gcgctccgac gttgaactat tgaacccttg aaccttgaac gaataactca
     2341 cgcccaataa gtcgttcgat ccatggggat atgctgcaag acttctcccg gcacgtagtc
     2401 tgccagcttg atccgcgcca tcgcctcgtc gggacacttc gacaccggcg agaacggccg
     2461 gtcaagcggc ttggtggcgt cgacgatcat gcaggaagtc ttgatctcgt cgtcgaggga
     2521 cggatcgagc atgttgccgc ggaaaatcgg cggcgtgatc gaaatctgca gatgcggctg
     2581 tacccgggtg gcgatcgccc acaagacatc ggtcggatta aaaacgtcga tatcgtcgtc
     2641 gaagacaaaa acatttttcg cgtgatcgaa ggtcagcgcc gctgccgcgg cccggcccgg
     2701 atcgccttcg gacatttttt tcatggcaat gaaaatattg tagttcgcct ggcggccgag
     2761 cttgcacacc gccgtgacgc ccggcaccgc ctcgcggcag cgccgcaggt acgcgccctc
     2821 gcgcggcaga tccatccacg gcttgtcgcc ctccggcgta gtgactgact ggtacatggc
     2881 gcccttgcga taattaatcg cccgaacttc gtactccaca caacggacat agcgctgcgg
     2941 cccaagataa ccgggcgctt cgcc
//
LOCUS       DASQKS010000113         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5951717, whole genome shotgun sequence.
ACCESSION   DASQKS010000113 DASQKS010000000
VERSION     DASQKS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5951717"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VE170_02965"
     CDS             complement(<1..379)
                     /locus_tag="VE170_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HYJ14420.1"
                     /translation="MEKQRTWKEKLGDKIPTDLGREIDIFEQQMELRRTGKIDEKVFA
                     ETRLRRGIYGQRYDNGQRHDGNRVQKLRLSAELVKGPDTLWDAPGMMRIKIPFGGVTP
                     EQFETLADLAEEYSDGVAHVTTRQ"
     gene            complement(484..918)
                     /locus_tag="VE170_02970"
     CDS             complement(484..918)
                     /locus_tag="VE170_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYJ14421.1"
                     /translation="MKVGSKAYYGFLALVELASNYKIRRTTQVKEIARDQDIPSEYLG
                     QIMVLLNRARLVHGSRGPGGGYILARPPETITVREVIEILEGPFATLEMRARKKGGTG
                     ALATERLIQTWAKGVAAMEAVLDDTTLADLCKPEQESYMYYI"
     gene            1298..2323
                     /locus_tag="VE170_02975"
     CDS             1298..2323
                     /locus_tag="VE170_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14422.1"
                     /translation="MKKLSRFALLIIAVILPPIEDSIAAPGEAIISHASMSTSSIPLW
                     VTQRRNFFGKYGVNSKVVWVRGNPAQIATLVSGDTQIAYGGATTALAAAVGGRDMKII
                     ASLSSRESLDLVARPNIKSAKDLAGKRFGVQSVGGGVWKTATVWLEHFGMDERRDNIQ
                     MIVIGDVTVLAQSLEAGLIDVTVVPPFLSRRLADKGFTVLGRCEQTKLPSVGMTVLVE
                     RPYLQQNTETLQGVMMALIEGMAFIGHPRNKPAVLETLTKQFRMTDGAAAEGAYQDVA
                     TLVRLEEGRKPYVSLDGMRILQRLLAPQNPKIGSIKVENLIDSSIMKRIDDSGFFDKM
                     AVEYAGK"
     gene            2388..3443
                     /locus_tag="VE170_02980"
     CDS             2388..3443
                     /locus_tag="VE170_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14423.1"
                     /translation="MNRHETKFCWLFLLAVVLTEHPAFAQDNFYRGKTIRFVVAYEAG
                     GGFDVYTRTIARHFGKHVPGNPTTLVENMTGAVGFIAANHIYNQAKPDGLTVGHFVGT
                     LVLQQVLGNEAARFDGRKFGWLGVPVADHGVCALTKASGISSLEGWLASKELVKMGGH
                     GPGGVTSDTARILREAIALPVRVVDGYKCTGPIRLAAETGEIAGGCWAWESMKPTWSS
                     GFETGQVKVLLQTSLKGHPELKDVPIAINHAKTKDARQLLEILDHAFGTSFRSYSTPP
                     GIPKDRLAILQKAFMETMRDPEFLADAKKARLDLAPIDGPTTARTIAGFYDLDKAIIA
                     KLKKILLGDVSGRDTPL"
     gene            3440..>3626
                     /locus_tag="VE170_02985"
     CDS             3440..>3626
                     /locus_tag="VE170_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14424.1"
                     /translation="MTIETRLLSFKTNDNETLHGLLFTPRERKSEVALLLVHGVAMNF
                     YLPPLATFGQALAERGHH"
BASE COUNT          781 a         1083 c          975 g          787 t
ORIGIN      
        1 cctggcgggt cgtcacatgg gcgacgccgt cggaatactc ttcggcaaga tcggccaagg
       61 tctcgaattg ctccggcgtc acgccgccga agggaatctt gatgcgcatc atgccgggcg
      121 catcccacag cgtgtccggt cccttgacca actcggcgga gagtctgagc ttttgcaccc
      181 ggttcccgtc gtgacgctgg ccattgtcgt agcgctggcc gtagatgccg cggcgcaacc
      241 ttgtttcggc gaaaactttc tcgtcaatct tgcccgttct gcgcagctcc atttgctgct
      301 cgaagatatc gatctcgcgg ccgagatcgg tgggaatttt gtcgccaagt ttttctttcc
      361 aggtccgctg tttttccatg attcgcctcc ggcgattatt tctcgattgg ctttcagacc
      421 atgaactaaa agtaagttga ttggtttagt cagttattcc aacaaaaaat ttttgcgcca
      481 aacctagata taatacatgt aggattcctg ctccggctta cagagatcgg cgagcgtcgt
      541 atcgtccaaa accgcctcca tagccgccac gcccttcgcc caagtctgga tcaagcgctc
      601 ggttgccaaa gcgccagtgc cgcctttttt acgcgcgcgc atttccagcg tcgcgaacgg
      661 cccttccaag atttcgatga cttctctgac tgtgattgtc tccggcggcc gcgccaaaat
      721 atagccgccg ccgggcccgc gcgagccatg aaccaagcgc gcgcggttga gcaaaaccat
      781 gatctggccg agatattccg aaggaatgtc ttgatccctg gcgatctcct tcacctgggt
      841 ggtgcggcgg atcttataat tgctcgccaa ctcgaccaaa gcgaggaagc catagtatgc
      901 ttttgagccg accttcattg agtccaaccc tcaggaaact taaactaagt aattcagtta
      961 acaatgtcaa gaatagagtt ggtaggtttc cgcaagggtt tttcccgtat gagccgatgc
     1021 cattttgccg cccttagcgt tttgtgggct gcgccgaacg gccgctagaa acttttctaa
     1081 aatttctcgc attcactcga gacaacagcc gcggactctc gcgtggagcc tcgctgccct
     1141 tgcgggaaat aatttaattt cttcttttgc gtcctctgcg gccatttcgt cttctttcct
     1201 ttgctgtttt caatttgcgt ggttccatcg attgaagcta tatttcggca gactcggcga
     1261 cgctgctcgg tgttcaactc actcggaggc aggttcgatg aaaaagctta gtcggttcgc
     1321 tttactcatc atcgcggtca ttttgccgcc aatcgaggat tcaatcgccg ctcccggcga
     1381 ggcgatcatc tcccacgcca gcatgagcac ctcgtcgata ccgctctggg tcactcaacg
     1441 gcgaaacttc ttcggcaaat acggtgtcaa ttcaaaagtc gtctgggtgc gcggcaatcc
     1501 ggcgcagatc gctacgttag tttccggcga tacccaaatc gcctacggcg gcgcaaccac
     1561 ggctttagcg gcggcggtcg gcggccgcga catgaagatc atcgccagtt tgagcagccg
     1621 ggaaagcctc gatctggtcg cccgccccaa tatcaaaagc gccaaagatc tcgccggcaa
     1681 gcgattcggc gtacagagcg ttggcggcgg cgtttggaag accgcgacgg tttggctcga
     1741 acatttcggc atggacgagc ggcgcgacaa tattcagatg atcgtcatcg gcgatgtcac
     1801 cgtcctggcg caatccctcg aagccggtct cattgacgtg acagtggtcc cgccttttct
     1861 cagccgccgc ctggcggaca aaggtttcac agtattgggc cgatgcgagc aaaccaaact
     1921 gccgtcggtc gggatgaccg tgctcgtcga aaggccatac ttgcagcaaa acaccgagac
     1981 cctgcagggc gtcatgatgg cgctcatcga aggcatggct tttataggcc atccgcgcaa
     2041 caaacccgcc gtgctggaaa ccctaacgaa gcaattcaga atgaccgacg gcgctgccgc
     2101 cgaaggcgcc taccaggacg ttgccacgtt ggtgcgtctg gaagaaggcc gcaagcccta
     2161 tgtttccctc gacggtatgc gcatcctcca gcgcctcttg gcaccgcaaa atcccaagat
     2221 cggcagcatc aaagtggaaa acttgatcga ctcgagcatc atgaaacgca tcgacgacag
     2281 tggatttttc gacaaaatgg cggtcgaata cgccggcaag tagcgaaaat aaacaactgc
     2341 ggcccagggc aaatggccgt gcttgtttat tacggagggc aatcatcgtg aaccgtcacg
     2401 aaactaaatt ctgctggtta ttcttgctcg ctgttgtctt gactgagcat ccggccttcg
     2461 ctcaagacaa cttttaccga ggcaaaacca ttcgcttcgt cgtcgcttac gaagcgggcg
     2521 gtgggttcga tgtttacacg cgcaccatcg cacgccactt cggtaaacac gtccccggca
     2581 atccgaccac cctggtcgaa aacatgaccg gcgccgtcgg cttcatcgcg gcgaaccata
     2641 tttacaatca ggccaaaccc gacggcctca ccgtcggtca tttcgtcggc acgctggtct
     2701 tgcaacaagt gctcggcaac gaagccgcgc gcttcgacgg ccgcaagttc ggctggctcg
     2761 gcgtgcccgt cgccgatcat ggcgtctgcg cgctgaccaa agcaagcggc atcagtagcc
     2821 tcgaaggctg gcttgccagc aaagagctgg tgaagatggg cggtcatggc ccgggcggcg
     2881 tgacttccga caccgcgaga attctgcgcg aagcgatcgc tctaccggtg cgcgtggtcg
     2941 acggctacaa atgtaccggg ccgattcgtc ttgccgccga gaccggagaa atcgccggcg
     3001 gctgctgggc ctgggagtcg atgaaaccga cttggtcgag cggcttcgag accggccaag
     3061 tgaaagtgct gctgcaaacc tcgctcaagg gccatccgga gttgaaagac gtgcccatcg
     3121 ccatcaacca tgctaagacc aaagacgcgc gccagttgtt ggagatcctc gaccacgcgt
     3181 tcggcacgag ctttcgcagc tactcgacgc cgccaggcat accgaaggac cggctggcga
     3241 ttttacaaaa agctttcatg gagaccatga gagaccctga gttcctcgcc gacgccaaga
     3301 aagctcgcct cgacctcgcg cccatcgacg ggccaaccac ggccaggacg attgccggat
     3361 tctacgatct cgataaagca ataatcgcca aactgaaaaa aattctcctc ggcgatgttt
     3421 ccggaaggga tacgccgcta tgactataga aacccgatta ctaagcttca aaaccaacga
     3481 caacgaaact ctgcacggcc tgctgttcac gccgcgcgag cgcaagtccg aggtcgcgct
     3541 tctgctcgtc catggcgtgg cgatgaattt ttatctgccg ccgctcgcca ccttcggcca
     3601 agccttggcc gaacggggcc atcact
//
LOCUS       DASQKS010000114         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5100005, whole genome shotgun sequence.
ACCESSION   DASQKS010000114 DASQKS010000000
VERSION     DASQKS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5100005"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VE170_02990"
     CDS             complement(<1..514)
                     /locus_tag="VE170_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ14425.1"
                     /translation="MDLGLKGKVVLIAGASQGMGKAAAAGFAREGAKVAICARGQTAL
                     NETAEQIRKQTGGEVLAIVADMSKPEDIKKFVDDAAKHFGRVDVVVNNAGGPPPGTKL
                     PRFGFLGLTDDDWREMFEFNLLSAVRACRAALPLLVERDAGAIVNVSSVHGRQPSAVN
                     VDYGAAKAAMI"
     gene            complement(621..866)
                     /locus_tag="VE170_02995"
     CDS             complement(621..866)
                     /locus_tag="VE170_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14426.1"
                     /translation="MSCYDLSRLLFDLKMNEAVYQKASADFENVMTGYDLSNEEKNAL
                     RAGDPRKLRQLGVHGMLCLYIKRLNPQFRENIYWAQK"
     gene            complement(894..1760)
                     /locus_tag="VE170_03000"
     CDS             complement(894..1760)
                     /locus_tag="VE170_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14427.1"
                     /translation="MPLVSALAASHAPNILLEPGREWEDFMDLHYSMAPQGSVNRPTL
                     EQQKKLRQDAETAFATLRADLEQAKPDVLIVVANDQFVNFFWNNIPTFFVTVAERVQG
                     QFTRHKFDYQNHKELGKAIIRAGMEQGIDFSYGEHVELQHTQNVPLYFLLPQPTIPIL
                     PIYVNTWVDPAPSPRRCYQVGELIREVAHNAKERVAILATGGLSHFPGSPRIGEIDES
                     FDHKLLEVMRQGEGRSLVNYSVQDLLHAGDTEFLNWMVVIGAVGDAKATFTAYMPDFV
                     ATGWGFVSWKLV"
     gene            2659..>2884
                     /locus_tag="VE170_03005"
     CDS             2659..>2884
                     /locus_tag="VE170_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14428.1"
                     /translation="MKLFKRNSTGDSQRPLAPRCDHPLAVIVDPSERELSEFETMLAR
                     CPNCSQWQPGQGPIMAVLTKRQDSSRNNSDH"
BASE COUNT          664 a          846 c          759 g          615 t
ORIGIN      
        1 tgatcatcgc cgccttcgcc gctccgtagt cgacattgac ggcagatggc tgccgcccgt
       61 ggacggacga gacattcacg atggctccgg catcgcgctc gacgagcagc gggagagccg
      121 cccggcatgc ccgcaccgcg gacaggaggt tgaactcgaa catctcccgc cagtcgtcgt
      181 cggtgaggcc gaggaacccg aaccgcggga gcttggtgcc gggaggcggc ccaccggcgt
      241 tgttgaccac cacatcgacc cggccaaaat gtttcgccgc atcgtcgacg aatttcttga
      301 tatcctccgg cttgctcata tccgcgacga tcgccaagac ctcgccgccg gtctgcttac
      361 gaatctgttc cgcggtttcg ttcaatgccg tttggccgcg ggcgcagatt gccaccttgg
      421 cgccttcgcg cgcaaaacca gccgccgctg ctttgcccat gccctggctc gcgccagcga
      481 tcaaaaccac tttacctttg agtccgagat ccatgttttc gtttccccct ggtcgttttg
      541 aagttgccgg atactaacaa acacaccgcc gtgggtcaaa atcaccgtga atttgtcagt
      601 actgaatctc ttggacgatt ttacttttgc gcccaataaa tattttcgcg aaattgcgga
      661 ttgagccgct tgatgtagag acagagcatg ccgtgcacgc ccagctggcg cagcttgcgc
      721 ggatcgccgg cgcgcagggc gtttttctct tcgttcgaca gatcataacc tgtcataacg
      781 ttttcgaagt cggccgacgc cttctggtag accgcttcgt tcattttgag atcgaacaac
      841 aaacggctta agtcataaca gctcacaaat cctccgtgac gccgagcccc cggctaaacg
      901 agtttccagc tgacaaagcc ccaaccggtg gcgacgaagt ccggcatgta ggcggtaaag
      961 gtcgccttgg cgtcgcccac cgcgccgatc accaccatcc agttgagaaa ctcggtatcg
     1021 ccggcatgca agagatcttg tactgaataa ttgaccaagc tgcgcccttc cccctggcgc
     1081 atgacttcga gcagcttgtg atcgaaactc tcatcgatct cgccgatacg cggcgagcca
     1141 gggaaatggg acaaaccgcc ggtggcgagg atcgccaccc gctcttttgc attatgcgcg
     1201 acttcgcgga tcaactcgcc gacctgatag cagcgccgtg gcgatggcgc cggatcgacc
     1261 caggtgttga cgtagatcgg taagatggga attgtcggtt gcggcaggag aaaatacagc
     1321 ggcacattct gcgtatgttg caactcgaca tgctcaccgt aggaaaagtc gatgccctgt
     1381 tccatgccgg cgcgaatgat cgccttgccc agttccttgt gattttgata atcgaactta
     1441 tgacgggtga actgcccctg cacgcgctcg gcgacggtga caaaaaaagt cggtatatta
     1501 ttccaaaaaa aattgacgaa ttgatcgttg gcgacgacga tcagcacatc cggtttggct
     1561 tgctccagat cggcgcgcag cgtggcgaat gcagtttccg catcctggcg caattttttt
     1621 tgctgctcga gagtcggccg gttgaccgat ccctgcggcg ccatgctgta gtgcaagtcc
     1681 ataaaatctt cccattccct gccgggctcg agaagaatgt tcggcgcatg gctcgcggct
     1741 aaggcggaaa ctaggggcat agctcaaact cctccgtgca gtgagcgaaa tcctattctc
     1801 cgacctgaat aattgcaact atggaatggg actatggagg gacgccatcg ggaggggact
     1861 atgagacgag accggcgtgg ctgagcgggc ggttcttgct ccggctaacc gccagcccaa
     1921 tgaccaatcg cttaaagcat ccgataattg cgcacggaca cccagaccgc aacaaagaca
     1981 acaacgagcg aaacaatcgg atgattgatg acccacgcga gcgacggttc aagagcatgg
     2041 cgcgaaacca aatcgaagta ccgtgataga atatccattt gtgcctctgt ctcgttgatt
     2101 tctggttatt ctacagcaac cgccgttatc cgaataccac aaagtggcca acacgaatcg
     2161 ctcgcgctat catggtcgcc accccacgac cttacagaga gataactgtg gcaatcttgg
     2221 atagttaatg caggtttcta tcaggttccg tacaatcttg gcttagcggc ggacaattct
     2281 gaaagctcgc cgctgccttt cacccgccat ggtccaggcc agccgtccgt aaatgacgag
     2341 attcgcgagg atttagccgg tccgacgagg tttcgagagt ctggcaccat ggttgctctc
     2401 tcgccggtat atcaacgcaa acgtaatccc atccgatgaa acttttactc gtaaccatgt
     2461 tcaggaaagt gcccgtgcgc aggaccaagc agcgtgccac gctatgcaaa cagtgcggca
     2521 caagaattta ttcattgacg tttctcaagg cccatctcga gtcccatcaa ggaattcgca
     2581 cttaactcga aatggagaca tggccgacgc cactttgact aataacaatc aagcgacgtg
     2641 acggccgctc aacaaaacat gaaactattc aaacgcaact ccaccggcga tagtcagcga
     2701 ccgctcgcac cgcgctgcga tcatccgctc gcagtgatcg tcgatcccag cgaacgggaa
     2761 ttgtccgaat tcgaaaccat gctcgcgcgt tgtcccaact gcagtcaatg gcagcctggg
     2821 caaggtccga tcatggcggt gttaaccaag cgccaagact ctagccggaa taactccgac
     2881 catt
//
LOCUS       DASQKS010000115         4748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5100074, whole genome shotgun sequence.
ACCESSION   DASQKS010000115 DASQKS010000000
VERSION     DASQKS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5100074"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..557
                     /locus_tag="VE170_03010"
     CDS             135..557
                     /locus_tag="VE170_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ14429.1"
                     /translation="MLKKILAPTDFSELSGEGVRYACLLANEVGAQVIVLNVVVLDET
                     NTVADGEIEAHKERLTAFVGKAVPHHGADVKLREVVLAGQPYSAIVDCAENEKIDLIV
                     MSSHGRSGLSRMLIGSVTDRLLRAAKCPVMVVPSLGKH"
     gene            982..1989
                     /locus_tag="VE170_03015"
     CDS             982..1989
                     /locus_tag="VE170_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14430.1"
                     /translation="MKSPRTATAWIVIALVVMLYGLAFTQERPSLLVGHGTLSGSILP
                     LWVGAEAKLFDKHGIQVKPIYLPRAAGRPALLAGEIQIYFSAGPPLVQMRLSGGDVAI
                     TSCAAHKLTSKIMVVSAIQKAADLRGKVFAVANPGSASDFAAKLFLARQGLKPGQDVS
                     LIYSGSTSAAFAALVNGRVQAIFTTAPNDLQAAAAGFKSLLELGDLNIPYIGNCSAAM
                     RPYIAKHPGRMRAFVAGITEAIAYIHRRPNEAKAVLEKYTRVSDPTILQHTYDSDTRF
                     MEPVPRPSIEGTRSVLEHLGVSGRPADQFVIDFIDDRFMKQIVDEGLLKQLYPGGIPA
                     R"
     gene            complement(2139..3653)
                     /gene="gndA"
                     /locus_tag="VE170_03020"
     CDS             complement(2139..3653)
                     /gene="gndA"
                     /locus_tag="VE170_03020"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HYJ14431.1"
                     /translation="MAEQNNSDVGLIGLAVMGQNLALNMADHGFKVAVFNRTTKVTHE
                     FVDANPDTPGGLIGCETLADFLAAVKTPRKIVILVKAGPAVDIVTTQLIEAGLAKEDI
                     VVDCGNSQWTDTIRREKEYSDRCRFFGSGVSGGEIGARFGPSLMPGGDAQAWKLLEPI
                     WTAIAAKVDAATGKPLETATPGHPVTGGIPCSAYIGENGAGHYVKMIHNGIEYIDMQL
                     ISEAYFLLRNLLQISAEELSRIFASWNEGDLDSYLIEITADILKQRDPERPDAFLVDS
                     VLDSAEQKGTGRWTSINALDLGIPTNSIAEAVFARCLSALKSERETASRQLAGPVYRY
                     DGDRNELIGSVHDALYCSKICAYAQGFQLMRAAQSEYGWKLDFASIASIWRGGCIIRA
                     RFLQKITDAYDGNARLVNLLLDPYFKEQLQNNQTRWRSVVAIAAQAGIAAPAFMSALA
                     YYDGYRSAKLPANLLQAQRDYFGAHTYERIDQPRGVHFHLEWSKPDRPQIKITH"
     gene            3880..>4748
                     /gene="corA"
                     /locus_tag="VE170_03025"
     CDS             3880..>4748
                     /gene="corA"
                     /locus_tag="VE170_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HYJ14432.1"
                     /translation="MAELLQGGTNLWFHFDAPTEAELRFLQDDLKIHHLTLEDVVNQN
                     QRPKIESFEDYVYLAIHPLRHTKKWQIEPLELDLLLGRHWIASVHYGPLPGIIENGHI
                     HERLANALGRGPDFLLYTLVDLVVDSYFPILDEIEDEIDSLEERLLAQPKSADMNRLL
                     AFKRSLVHVRRAVGPQREVFNQLTRHEFPFIRAENLVYFRDVYDHLLRITEELDSLRD
                     ILSSALEIHLASASNQLNVTMKRLTAWGTIFVVITAIAGIYGMNFKFMPELEWRYGYL
                     AAISGMILISLGL"
BASE COUNT         1031 a         1309 c         1283 g         1125 t
ORIGIN      
        1 cacagctgct actgctccgc tcgccgccgc gtcgattggc gattgcggta gagctgctcg
       61 gcgtgagcga ttttcgcgca atctcgcgca gtgactcggc ggttgacggg ctgtagaaag
      121 gcttcgctgg ggccatgcta aagaagattc tcgcgcccac cgatttctcc gagctctcgg
      181 gcgaaggagt acgctatgcc tgccttttgg ctaatgaagt cggggctcag gtcatcgtcc
      241 tgaatgtcgt tgtcctcgat gaaaccaata ccgtcgccga tggtgagatc gaagcgcaca
      301 aagaacggct caccgccttt gtcggcaagg ctgttcccca tcacggtgcc gacgtgaaac
      361 ttcgtgaagt ggtccttgcc ggtcagccct acagcgctat cgtcgattgc gccgagaacg
      421 agaagatcga tctgatcgtc atgtcgtccc atggacgcag cggcctgtca cgcatgctga
      481 tcggcagcgt tacggatagg ttgctgcgcg cggcgaaatg tccggtgatg gtggtgccgt
      541 cgcttggtaa gcactaagtg tgactgatta ggacctttgc ctcgtgaaac gttgacctct
      601 cgctatgggg cggggtaaag tggatttccg cataaatcca tcgcatgacc gccctcgcgt
      661 tgacgcttgc cgcggctttt tcttagggga catggttttt atactgctcc cgccaaccat
      721 tttattaaga agaattagga gcaagttcaa atctcttatc taaggcgtaa gcccgattct
      781 ccgacaaagc cagcctgact cgctgattcc cgccagcatt cgcgggctgc gcccagcgtg
      841 tctcccaaac ggttacgccc atatccaatg agctgcgatt gaaactatct ttctcgatag
      901 gctatctgtt gtcgcaaatt agctcgaccc aaccgacggt gcgagtttcc tgagacgatc
      961 aagcatatcg gaggccgacc catgaaatcc ccgcgaactg ctacggcatg gattgttatc
     1021 gcgttggtcg ttatgctcta tggccttgct tttacccagg agcggccgag tctgcttgtc
     1081 ggtcatggaa cattatcggg ttcgatcttg ccgctgtggg tcggcgctga ggccaagctg
     1141 tttgacaagc atggcattca ggtcaagcca atctatttgc cgcgtgccgc gggccggccg
     1201 gcactgctcg caggcgagat tcagatttat ttctctgccg gtccgccgct ggtgcagatg
     1261 cgtttgagcg gcggcgacgt cgcgattact tcttgcgcgg cgcataagct gacttcgaag
     1321 atcatggtcg tttcggcgat tcaaaaagcc gctgatctgc gcggcaaagt atttgccgtt
     1381 gccaaccccg ggtcggccag cgattttgcc gccaagttgt tcctcgcccg ccagggactc
     1441 aaacccggtc aggatgtttc gctaatttac agcggatcga ctagcgcagc ctttgcggcg
     1501 ttggtcaacg gccgagtaca ggcgattttc acgaccgcgc cgaacgattt gcaagcggcg
     1561 gcggcaggtt tcaaatcgct gctcgaattg ggcgacttga atatccccta catcggcaat
     1621 tgcagcgcgg cgatgcggcc gtatatcgct aagcaccccg ggcgtatgcg cgctttcgtt
     1681 gccggcatca ccgaagcgat cgcctatatt catcgacgtc ccaatgaggc gaaggcggtg
     1741 ttggaaaaat acactcgagt ttccgacccg accattttgc aacacaccta tgactccgac
     1801 acccgattta tggagccggt gccccgtccg agtatcgaag gcaccaggag cgttctcgaa
     1861 cacctgggag tcagcggccg gcctgccgat cagtttgtca ttgatttcat cgacgaccga
     1921 ttcatgaaac agatcgtcga cgaaggtttg ttaaaacagc tctacccagg cggtatccca
     1981 gcgcgataac cttcgcttcg cccgatgcag caagcgctaa agagcgaaag tcgatggcgg
     2041 tcaaacgtag aaatgttacc aacgccggtt gccacctgtt cgtatcgcga gggcgttgaa
     2101 cgcgaatcat gccgatgagt aggagtattg ccaaaagctt aatgagtaat tttgatctga
     2161 ggccggtccg gtttggacca ttcaaggtgg aaatgcacac ctcgcggttg atcgatacgc
     2221 tcgtaggtgt gggcgccgaa ataatcccgt tgagcttgaa gaagatttgc cggaagtttt
     2281 gccgatcgat aaccgtcata ataggccagg gccgacatga acgcaggtgc ggcgatgccc
     2341 gcctgagccg cgatcgccac gacagagcgc cagcgcgtct gattgttttg tagctgttct
     2401 ttaaagtagg gatccaggag cagattcacc agccgcgcat tgccgtcgta agcgtcggta
     2461 attttttgta gaaagcgtgc ccggatgatg catccgccgc gccaaatgct cgcgatcgag
     2521 gcaaaatcca gtttccatcc atattcgctt tgagccgccc gcatgagctg aaagccctgc
     2581 gcgtaggcgc aaatttttga acaatataag gcgtcgtgca cggatccaat tagttcattc
     2641 cgatcgccgt cgtaccgata cacggggccg gcgagctgtc gggaagccgt ctcacgttct
     2701 gatttcaacg cgctgagaca gcgggcaaag accgcttcgg caatcgagtt tgtcggtatt
     2761 ccgagatcga gcgcattaat gctcgtccac cgtccggtgc ctttttgctc cgcactatct
     2821 aagacagagt cgacaagaaa agcgtcaggc cgttccgggt cccgctgctt aagaatgtca
     2881 gcggtaattt caatcagata ggaatccaaa tctccttcgt tccagcttgc gaagatgcgc
     2941 gaaagttcct cggcgcttat ttgtaagaga ttgcgaagca ggaaataggc ttcactgatc
     3001 agctgcatat cgatgtattc aatgccgtta tgaatcatct tgacgtaatg tcccgcgccg
     3061 ttttcgccaa tgtaagccga gcagggtatg ccgcctgtta ctgggtgtcc cggcgtcgcg
     3121 gtttcgagcg gtttgccggt ggcggcatcg accttggcgg cgatggcggt ccaaatgggc
     3181 tccaaaagtt tccatgcctg ggcatcgccg ccgggcatga gtgacggccc gaaacgagcg
     3241 ccgatctcgc cgccggagac tccggaaccg aaaaacctgc atcggtcgga atattccttt
     3301 tcacgccgga tcgtatcggt ccattgactg ttgccacaat caaccacgat atcctctttt
     3361 gccaggccgg cctcgatcag ctgggtcgtg actatgtcca cggcgggtcc agccttcacc
     3421 aatataacga tcttgcgcgg ggtcttcact gcggctagga aatccgcgag agtctcgcat
     3481 ccgataagcc ctccgggcgt atcaggattg gcgtcgacaa actcatgggt caccttggtt
     3541 gtgcgattga ataccgccac cttaaatccg tgatcagcca tattgagcgc cagattttgg
     3601 cccatcaccg ccaaaccaat aagcccgaca tccgagttat tttgttccgc catatgcttt
     3661 tccttccggt tcaactaaag cgattgatac attacagaaa ctttctgaca tcgtaacgaa
     3721 aaattagcga ctccgtaagc taagtagctt ggaggagcgg atactgcggc gcgaacaatc
     3781 gcttcggcaa caagttacgt tcatgctata tacaattcac ccatgctaac catccgccgc
     3841 gcaaccggaa atggccttga gacggtcaat caaaatgacg tggcggaact tttacaaggc
     3901 ggcacaaatc tctggtttca tttcgatgcg ccgacagaag cggagctgcg ctttctccaa
     3961 gatgacttga agatccatca cctgactctt gaagatgtcg tcaatcagaa ccaacggcca
     4021 aaaatagaat catttgagga ttatgtttac ctggcgattc atccgttgag gcacaccaag
     4081 aagtggcaga tcgagcctct cgagctcgat cttttgctcg gccgccactg gatcgcctcg
     4141 gtccattacg gccctctgcc gggcatcatc gagaatggcc atatccatga gagactggcc
     4201 aatgccctcg gcaggggccc tgacttcctt ctctatactc tcgtcgatct ggtcgtcgac
     4261 agttacttcc cgatcctgga tgaaattgaa gacgaaatcg attctcttga ggagcggctg
     4321 ctggcgcagc cgaagtcagc agatatgaat cggcttttgg cattcaagcg ttcattggtt
     4381 catgtccgtc gcgccgtcgg gccgcagcgg gaagttttca accagctaac gcgccacgaa
     4441 tttcccttca tccgcgcgga aaatctggtt tatttccggg acgtttatga tcatttgctg
     4501 cgcatcaccg aagaactcga cagtctcaga gatattcttt ccagcgcgct agaaatccat
     4561 ctcgcgtccg cgtcgaacca gctcaatgtg acgatgaaaa gactcaccgc ctggggtacg
     4621 atattcgtcg tgatcaccgc catcgcgggc atctatggca tgaatttcaa attcatgccc
     4681 gaactcgagt ggcgctatgg ttatctggcg gcgatctccg gtatgatttt gatctcgctg
     4741 ggtttgta
//
LOCUS       DASQKS010000116         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1320239, whole genome shotgun sequence.
ACCESSION   DASQKS010000116 DASQKS010000000
VERSION     DASQKS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320239"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VE170_03030"
     CDS             complement(<1..1126)
                     /locus_tag="VE170_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14433.1"
                     /translation="MAEENIVSRRKFLGAVAAVGAVGLSACATAGQSSSKLWTADRSS
                     GQLPPRGEFVVRNAYVVTMDPKLGDIPNGDVHVRNGALVAVGANLNAPGAEVIDGRQR
                     IACPGFIDTHFHLWGSFARGVVADGDFDYFPVMSKLGPVMTPEDAYASARLGITEAVY
                     SGLTTIHDWSHNIIDGRYADADLRALRDCGIRARFSYGYSRNLQLRPKETADFNDIAR
                     VKREWFGASNDGLTTMGFASRGAGDTPPEVYRKEWENARSLGLPITQHAGRSLAEIKR
                     FRRIEMLYNDKLLGPDVQLIHTYSASERERAMMAETKVHNSIAPYTASRLASGLPYLG
                     DLLKRGVQCSLSVDTTTVGGNADMFSIMRLMLQLNHLGSMD"
     gene            1382..>3387
                     /locus_tag="VE170_03035"
     CDS             1382..>3387
                     /locus_tag="VE170_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ14434.1"
                     /translation="MGCLVLALSLALVQLAPAADGAPRTRLIVGSELDFPPFALVREG
                     GRADGFTAELWQAVAREAHLDATITTGPFHQLLDQFKSGEIDVLINLAQSDERRRFAD
                     FSVPHVKMAGAVFTRAGDSRIKSEHDLARLSLIIINADLAYEYARRRGWRNLTLVDTA
                     ASGMKLLETSTKHDAMLVGRLVGLNTIRELKLANIEPLRLKVGYQQDFAFAVRKGNAE
                     LLARINDGLANVRASGAYDTIYGKWFGRLEPVPLTMESILKYLIPAVGLILLLLLAYL
                     HERRLRVRWKTTAAALDATITERIQAETALRVSEERFKAFMDRSPAIAFIKDETGRYV
                     YVNKTWEDLYTIDWFGKTDAELWPRADADMFTASDRRALAAGHTIETFETVLDKKGRP
                     QDWWVLKFPIVDADGIRLLGGIAMDIGERKAAEQALLASETKLRRITQTIPGAVYQYQ
                     LSADGTQKFISISDGIENLIGHPAAAVANDFGLFWNGVPAEDQEAWTTSILRSAQTML
                     PWEFEGRIRLTTGALKWFRGHSVPGPRQPDGSIVWIGLLTDVTRRKRNEGERARLAAI
                     VDNSGDAIISRDLDFKILTFNAAAERLLGYAAVEVIGKLSDIFIPQDNFAAVAQRRSL
                     IHQGLQPAAADSVWLRRDGRRIDVSVTQSPIKDASGKAIGVSLSV"
BASE COUNT          661 a          965 c         1060 g          701 t
ORIGIN      
        1 cgtccatcga gcccaggtga ttgagctgaa gcatcaatcg catgatgctg aacatgtcgg
       61 cgttgccgcc caccgtcgta gtgtccaccg acaagctgca ttgcacgccg cgttttagca
      121 aatcgccgag gtagggcagt cccgaggcca agcgcgaggc ggtgtacggc gcgatgctgt
      181 tgtgcacttt ggtctccgcc atcatggcgc gttctcgctc cgacgcgctg taagtatgaa
      241 tgagctggac gtcggggccg agcaatttgt cgttgtagag catctcgatg cggcggaagc
      301 gtttgatttc cgccagagag cgtccggcgt gctgggtgat tggcaagccg aggctgcggg
      361 cattctccca ttctttacgg taaacctccg gcggcgtgtc gccggcgccg cgggaggcga
      421 agcccatagt ggttaggccg tcgttggacg cgccgaacca ttcgcgcttc actcgggcga
      481 tatcattgaa gtcggcggtc tctttgggcc gcaactgcag gttgcggctg tagccgtaag
      541 aaaagcgcgc gcggataccg cagtcgcgca gcgcgcgcag gtcggcgtcg gcgtaccggc
      601 catcgataat gttatgcgac caatcgtgga tcgtcgtcag tccggaataa acagcttcgg
      661 taatccccag ccgtgcgctg gcgtaggcgt cctccggcgt catcaccggc ccgagcttgc
      721 tcatgaccgg gaagtaatcg aagtcgccgt cggcgaccac gccgcgggcg aagctgcccc
      781 agagatggaa gtgggtgtcg atgaacccgg ggcaggcgat gcgttgccgt ccgtcgatga
      841 cctcggcgcc cggcgcattc aaattcgcgc cgaccgccac cagcgcgccg ttgcgcacat
      901 ggacatcgcc gttggggata tcgcccaact tggggtccat agtgactaca taggcattgc
      961 ggacgacgaa ctcgccgcgc ggcggcagtt gtccggagct gcggtctgct gtccacagtt
     1021 tggatgacga ttgaccggcg gtggcgcaag ccgacaaccc gactgcgccc accgcggcaa
     1081 cggcgccgag aaatttgcgg cggctgacga tgttttcttc ggccatgatg gttccctttc
     1141 ggctcaggaa aaatcttctt gagtttttta gtcgataaca atccgcaccg tcaagaaaat
     1201 aacgatgcga gaatcagcaa taattcagaa tttttttgtt gcgaaccctg gcatgcctcg
     1261 atacattgat cgcctcaaag tgaattctga cgagcgcctg atgtttttaa cacacaagtt
     1321 tgtcatgcat tgctctggga tcgtttccag tgttgcgatg tcatggccgc ggcggcgaag
     1381 catgggttgc ttggtcttgg cgcttagtct ggcgctagtc caactggcgc cggccgccga
     1441 tggcgcgccc aggacgcgtt tgatcgtggg cagcgaactt gattttccac cctttgccct
     1501 ggtgcgcgaa ggcggccgcg ccgatggctt tacggcggag ttgtggcagg cggtcgcccg
     1561 cgaggcacac ctggacgcga cgattacgac cggtccattt caccaactac tcgaccagtt
     1621 taagagcggg gaaatcgatg tgctgattaa cctcgcgcag tccgatgagc gccggcgctt
     1681 cgccgatttt tccgtgcccc atgtcaagat ggccggcgcg gtctttaccc gcgcaggtga
     1741 cagccggatc aagtccgagc atgacctggc gcggttgtcg ctgatcatca taaacgcgga
     1801 tctggcctac gagtatgcgc gccggcgcgg ttggcgcaat ctaacgctgg tcgatacggc
     1861 ggccagcggc atgaagttgc tcgagacctc gactaagcac gacgcgatgt tggttggccg
     1921 gctggttggc ctcaatacca tccgggaact gaaactcgcc aatatcgagc cgctccggct
     1981 caaagtgggc taccagcagg atttcgcatt tgccgtgcgc aagggcaacg ccgagctctt
     2041 ggcccggatc aacgacggcc tggccaacgt ccgcgctagc ggcgcctacg acacgatcta
     2101 cggcaagtgg ttcggccgct tggagccagt gccgctaacg atggagagta tattaaaata
     2161 cctgattccg gccgtcggac tgatcttgtt gcttcttttg gcctacctgc atgagcgtcg
     2221 tttgcgcgtc cgctggaaga ccaccgccgc cgcactggac gcgaccatca cggagcgcat
     2281 acaagcggaa acggcattgc gcgtaagcga agagcgcttc aaggcgttca tggaccggag
     2341 ccccgccatt gcgttcatca aagatgagac tggccgctat gtgtatgtga ataaaacctg
     2401 ggaggatctc tatactattg actggttcgg taagactgat gccgaactct ggccgcgcgc
     2461 agacgccgat atgtttaccg cgagcgaccg acgtgctctc gctgccggtc acaccatcga
     2521 gacctttgag acggtattgg acaaaaaggg gcgaccccag gactggtggg tgctgaagtt
     2581 tcccatcgtc gatgccgacg gcatccgatt gctcggcggc atcgccatgg acatcggcga
     2641 gcgcaaagca gccgagcaag ctttattggc cagcgaaaca aaattgcgcc ggatcaccca
     2701 aaccattccc ggagcggtat accagtacca gctgagcgcc gacggaactc agaagtttat
     2761 ttcgattagt gacggtattg agaatcttat cggacacccg gcggcggcgg tggccaatga
     2821 ctttggttta ttttggaatg gtgtgccggc cgaggatcaa gaggcgtgga ccacgtctat
     2881 cctgcgttcg gcgcagacga tgctgccgtg ggagttcgag gggcgcatcc gtttaaccac
     2941 cggcgcgttg aaatggttca ggggccactc ggtgcccggg ccgcggcagc cggatggcag
     3001 catagtgtgg attggactcc tcaccgatgt tactcggcgc aagcgcaacg aaggcgagcg
     3061 cgcccggctg gcggcgatcg tcgacaattc gggggacgcg atcatcagcc gggatctcga
     3121 ttttaaaatt cttaccttta atgccgccgc cgaaaggttg cttggctatg cggcggtgga
     3181 ggtcattggc aagctctccg atattttcat acctcaagat aactttgccg cggtggcgca
     3241 aaggcgcagc ttaatccacc aaggcttaca gccagccgcc gccgattcgg tctggttgcg
     3301 gcgagacggt cggcggatcg atgtatcggt gacgcagtcg ccgatcaagg acgccagcgg
     3361 caaagcgatc ggggtttcgt tgtcggt
//
LOCUS       DASQKS010000117         7933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1741780, whole genome shotgun sequence.
ACCESSION   DASQKS010000117 DASQKS010000000
VERSION     DASQKS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741780"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VE170_03040"
     CDS             complement(<1..908)
                     /locus_tag="VE170_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ14435.1"
                     /translation="MAKIIDSLVEYMLAARQVLLPAQVVQKGKSHLLDSLAAVVSGST
                     LKPGKLGLQHAREQGGREECSVLGTNFKTTPIMAAFANGMSGHADETDDSNSQLHPGC
                     AIVPAALALAESQDRGGEALLRAVILGYDIGFRFHQAFAPRSTTFGATFGCAAAAGTL
                     AALDAMQMGYLISYAAQQASGSRAWVGDDDHIEKAFDYAAMPARNGVTAALLIKSGFT
                     GNRDVLEGDQGIIKTYAPCDPAKILDELGQRFTITSCLVKKYPVGSPMMETVDATLAM
                     LAKQSVNADDISKVVVRIPESGARTV"
     gene            1072..2607
                     /locus_tag="VE170_03045"
     CDS             1072..2607
                     /locus_tag="VE170_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14436.1"
                     /translation="MHRSSWCSSRGKFFHRIFVVIAVFLAIGASARLSSAQSRSRVIV
                     AHGVGIYSLNPYAVNTSPLVAAWGSLMESLIEPDYEKRGYRGVLAESWQLKGTRLEFK
                     LRKGVRFHDGSPFTAADVIASFKRILTDKNSLQVSNLENVKDMEAPDPHTVIINLKRL
                     DANILEDINNRAIMKQATAEKMGEADDRPIGTGPFKFTSWERSGQFVIRRNENYWGRP
                     AKIDEVVYKAIQEDAARIAALESGQVDVISNIPPHEVGRLKANPRLRVQQVQGLRPLF
                     LVLSPHYKPLDNPKLRRAMSHAIDRERIIKHILEGNAYPLSGMLSPQVFGYDAGAKAF
                     AYDPEKAKQLLVEAGFPNGFEVDYFSPTGRYPKDKEVALVIVEQLARVGIKANLKTPE
                     WAIFNTDYKKGKYPMYLTGRGSLVDADALFQQYFRTGVTPRVLGYSNPKLDEILDLEQ
                     QTFDSKKREKLLQEAHRIILDDAPAIPLWNAMDIYAHRADLIWTAPPDEKVQLKQASF
                     KTK"
     gene            complement(2629..3054)
                     /locus_tag="VE170_03050"
     CDS             complement(2629..3054)
                     /locus_tag="VE170_03050"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HYJ14437.1"
                     /translation="MPCQAHLGEVRQRHGEFGRRGVAVAVVSFANPEQLAQYQQRHRW
                     PFIMLADPERQAYNAFALKRLSWFQVFSPAALKLYWKLLRGGMGQEAYRGDDIYQSGG
                     DFLLDREGNILFAHRSQNPADRPAPAQLLMVIDRIQRAP"
     gene            complement(3407..3757)
                     /locus_tag="VE170_03055"
     CDS             complement(3407..3757)
                     /locus_tag="VE170_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ14438.1"
                     /translation="MSSKIYVGGLPYATTDAQLQDLFSTHGAVESARVITDKFTGRSR
                     GFGFVEMANSDEAQKAIQALNGTDFEGRNLTVNEARPQERRPGGGGGGFGDRGGERRG
                     GGGGGGGGGGRNRW"
     gene            4094..4849
                     /locus_tag="VE170_03060"
     CDS             4094..4849
                     /locus_tag="VE170_03060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ14439.1"
                     /translation="MYQTNEYEGMIAETVTMQGHKGETINAYMARPLGAGPFPGMVLV
                     HHAPGWDEWYRECTRRFAHHGYATISPNLYFRDGHGTAEDVGAKVRAAGGIPDEQVLG
                     DLDGANKLLRSMPYLNGKVGIFGTCSGGRHSFLAACRTKGIDALVECWGGGVVMKADE
                     LTPKKPVAPIDYTKDLSCPMIGIFGNDDKSPSPEQVNAHEAELKKQGKKYEFHRYDGA
                     GHGFFYYDRPAYRQEQAVDGWKKVMAFCEKNLK"
     gene            4867..5157
                     /locus_tag="VE170_03065"
     CDS             4867..5157
                     /locus_tag="VE170_03065"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HYJ14440.1"
                     /translation="MCTSITHQAKIYGRGKSGQEWIELKEANVSYDHPYGMPLEHALN
                     IDFVDESKGPGARVAVELSVDAARRLVQTIQAVLAQAEHQGVLEDKHQAAVG"
     gene            5279..6529
                     /locus_tag="VE170_03070"
     CDS             5279..6529
                     /locus_tag="VE170_03070"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14441.1"
                     /translation="MAAAEEITKSAEAAPTRPWASLAIRDYRLMWIASVCNAVAIQVR
                     NVAGLYQVYEISGSALQLGLTGFFQAFPFVIFGLFAGAVADAFDRKKLLVVTVAIQLI
                     PSLLLGFLTMAGTIQVWHIFALGFVGALVEVFNWPARSALIPRLVPPSYMMNAVTLNT
                     IIIQTSFLLGPALGGLLIDHSGLVFTYFLTIAMFIPSGIAIFSMRTSGVPEGERRRVN
                     LKSIFEGIEFIWVQRIILSLFLLDFGVTLVGFYKPILPIFAADVFKTGASGLGILYGA
                     PSLGAIIGSIVLLMIGNVNRMGVLVVCAALIFAGSLTLLGLSNSFWLAVGATLILGFT
                     DSVSVAIRRTVVQMMAPDGMLGRASSLITAFAQATNGLGAVLAGAAAQQVGAPNALLL
                     GSALCFVMILAICLAIPQLWRYRS"
     gene            complement(6834..>7933)
                     /locus_tag="VE170_03075"
     CDS             complement(6834..>7933)
                     /locus_tag="VE170_03075"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ14442.1"
                     /translation="VIKIEHPKYGDGQRKLEPIMDGIPLWWKSVARNKRCITLDLGKP
                     EGADIFKQLVKGTDVIVENYRPGTFEKWGIGYDAIRAIDPRIIMLRISGFGQTGPYKN
                     RAGFGRVAEAMSGLTNLIGEPDGPPMSPGYPLGDLIAGIFGSLSVMMALYHRDLRGGE
                     GQMIDLALYEAVFRFLDFDPIQYDQMKIVHKRTGNRVAYVAPSSMFKTKDGKYLTLAA
                     STQSVWLRLAEAIGRKDMTTDPKFLDNSARVENSIECNGVVGAWIEQHSRDEVIEHFD
                     KHGVAYSAVFDMEDAFRDLQYRAREAMVRVPDPDLGEAIVQNVVPKFSATPGSVDFLG
                     HKLGEDNDAILGGELGLSKEKLQELKDAGII"
BASE COUNT         1661 a         2377 c         2205 g         1690 t
ORIGIN      
        1 ttgaccgtgc gcgcgccgct ttcgggaatg cgcacgacca ccttgctgat atcatcggca
       61 ttaaccgatt gcttggcgag catcgccaga gtcgcatcga cggtttccat catcggcgaa
      121 ccgaccgggt atttcttgac caggcagctc gtgatggtga agcgttggcc gagttcgtcg
      181 agtattttcg ccggatcgca gggagcatag gtcttgatga tgccctgatc gccctcgagc
      241 acgtcacgat tgccggtgaa gcctgattta atcaaaagcg ccgccgtgac accgttgcgc
      301 gcgggcatgg ccgcgtaatc gaaggctttt tcgatatgat cgtcatcacc gacccaggcc
      361 cgtgatcccg acgcctgttg cgccgcatag gaaattaaat aacccatctg catggcgtcc
      421 aaagcagcca gcgtgccggc agcggcggcg cagccgaacg tcgcgccaaa tgtcgtcgat
      481 cgcggcgcaa aagcttgatg gaagcgaaag ccgatgtcgt acccgagtat aaccgcgcgc
      541 agcaaagctt cgccgccgcg gtcctgcgac tctgccaacg ccagtgcggc gggcacgata
      601 gcacagccgg gatggagctg actgttggaa tcgtcggtct cgtcggcgtg gcccgacatg
      661 ccattggcaa aagccgccat gatcggcgtc gttttgaaat tcgtacccag cacgctgcac
      721 tcttctcggc cgccctgctc gcgcgcgtgt tgcaggccaa gcttgccggg cttcaatgtc
      781 gagccggaga ccaccgctgc cagcgaatcc aacagatggc ttttgccctt ttgcacgacc
      841 tgcgccggca gtagaacctg tcgcgccgcc aacatgtatt cgaccaagga atcgatgatt
      901 tttgccatgg cgcctcctgg aagtgaaccc atactggact actgaagtgt cgcccggctg
      961 tcaacataaa gttgacagcg atccatcctg tgggttagcg tggccgaaca ttcagtggaa
     1021 taagcagccg tcgaactagc actgcagtca tcgttgcaaa cggaggtttc gatgcacaga
     1081 agctcctggt gctctagccg cggaaagttt ttccatcgta tcttcgtcgt catcgcggtt
     1141 tttctcgcga tcggcgcgtc tgcacgcctc tcatcagccc agtcacgcag ccgagtcatc
     1201 gtcgcccatg gggtcggcat ttattccttg aatccttacg cggtcaacac ctcaccgctg
     1261 gtggccgcct gggggtcgct gatggagtct ctgatcgagc cggactatga gaaacgcggc
     1321 taccgcggtg tgttggccga atcctggcag ttaaaaggaa cccggcttga gttcaaattg
     1381 cgcaagggcg tgcgcttcca cgacggctcg ccgtttaccg ccgccgacgt catcgcctcg
     1441 ttcaaacgta tcctcaccga caagaacagt ttgcaagtgt ccaatcttga gaacgtcaaa
     1501 gacatggagg cgcccgaccc ccatacggtc atcattaact tgaagaggct cgatgccaac
     1561 attctcgagg acatcaacaa ccgggccatc atgaagcaag cgacagcgga aaaaatgggc
     1621 gaggctgacg atcggcccat cggcaccggc ccctttaaat tcaccagttg ggagcgcagc
     1681 ggccaattcg taatccgccg caacgaaaat tattgggggc ggccggcaaa aatcgacgaa
     1741 gtggtttaca aggccataca ggaagacgcg gcgcgcatcg ccgccctcga atccggtcag
     1801 gtcgatgtca tcagcaacat cccgccgcac gaagtcggcc gtttgaaagc caatccgcgg
     1861 ctgcgggtgc aacaagtgca gggactgcgg ccgctctttc tcgtgctcag cccgcactac
     1921 aaacccctcg ataatccaaa actgcggcgc gccatgagcc atgcgatcga tcgcgaacgg
     1981 attattaaac atatactcga aggcaacgcc tatccgctga gcggcatgct cagtcctcag
     2041 gtgttcggct acgatgccgg cgccaaagct tttgcatacg acccggaaaa ggccaaacaa
     2101 ctgctcgtcg aagccggctt tcctaacggt ttcgaggtcg actatttcag tcccaccggg
     2161 agatatccta aagacaagga agtggccctg gtgattgtcg agcagcttgc ccgcgtcggc
     2221 atcaaagcca atctcaagac gccggaatgg gcgattttca acaccgatta taagaaaggt
     2281 aaatatccca tgtacctgac cggccgcggc agtctggtcg acgccgacgc gctttttcaa
     2341 caatacttca gaaccggcgt cactccccgc gtgctcggct acagcaatcc caaactcgac
     2401 gagattctcg atcttgagca acaaaccttc gacagcaaga agcgcgagaa actgctgcaa
     2461 gaggcgcatc ggataattct cgacgacgct ccggcgattc cattatggaa cgccatggat
     2521 atttacgccc accgcgccga tctcatttgg accgcgccgc cggacgaaaa ggtgcaatta
     2581 aagcaggcga gcttcaaaac gaaataaccc gcgccgcgtc ttagtctttc agggcgcacg
     2641 ctgaatgcga tcgatcacca tcagcaactg tgccggcgcc ggccggtccg ctggattttg
     2701 gctgcgatgg gcgaagagaa tattgccttc gcgatcgagt aagaagtcgc cgccggactg
     2761 ataaatatca tcgcccctgt aagcctcctg ccccatgccg ccgcgcaaaa gtttccagta
     2821 caacttcaat gcggcaggcg agaatacttg aaaccaggac aaccgcttta acgcgaaagc
     2881 gttatacgcc tgacgctcgg ggtcggcgag catgatgaac ggccaccgat ggcgctgttg
     2941 gtattgcgcc agctgctccg ggttggcaaa cgacaccacc gcgacggcga cgccgcgccg
     3001 gccgaactca ccatgtcgtt gccgcacctc gccgagatgc gcttggcaag gcagtcaagc
     3061 caggtgacgc aggaaaatca gcaggagata ttggtcgaat tgtccggcga gactggccgg
     3121 ctggccgtcg agggtcagca gcggcaattg tgcgaggtcg atcttttcca cgtcgtacga
     3181 caaaatttgc ctggcttatc catgttgtga ttcctgaagg cgaccgccgg ggctatcgtc
     3241 tacagttcgg attccctttt gtgaacttgt gtggcccggt tccttcgcat ccgctcagga
     3301 caagctttgc ggccaatcat ccgaagcctt ttcatcttgg tgcaataaca aaaggggcgc
     3361 cccgacctag cgcccctttt cgttgaatgg aaatagtgac taaaaactac caacgattgc
     3421 ggccaccgcc gccaccgccc ccaccaccgc caccgcgacg ctcgccgccg cgatcgccga
     3481 aaccaccgcc gcccccaccg ggacgtcttt cctgtggacg agcctcgttg accgtcaagt
     3541 tgcgcccttc gaagtcggtg ccgttgagcg cctggattgc tttctgcgct tcgtcggagt
     3601 tggccatctc gacgaagccg aacccgcgcg aacgtccggt gaacttatcc gtgatcaccc
     3661 gggccgactc cactgcgccg tgggtcgaaa acagatcctg tagttgtgca tccgtggtcg
     3721 cgtacggcaa accacccacg taaattttac tgctcatgct ctctcctttt aaacatcggt
     3781 tatggtcttt gccctgagca ctgatcaccg ctggcagcag aacatggagg atagacacgc
     3841 tctggtcgga ttgtccgcgt ggcaccgaac tgcaaggtaa gacgccctag accttcaact
     3901 ttacttttat ttgtcactat cagaaggccc catactgatg tgtaaaaaaa gcttaactta
     3961 atcggcccgc cgcgtgcaag ggtaaaaagg ccgaaatttt cgctacttta aaggcctgga
     4021 catcggttgc agtagcggtg attttcgtat taatgaggtc tgatcttagg aattttttaa
     4081 ggaggagtag acgatgtatc aaaccaacga gtacgagggc atgatcgccg aaaccgtgac
     4141 catgcaaggg cataaaggcg aaaccatcaa tgcttacatg gcccgcccat taggcgccgg
     4201 accgtttccc ggaatggtcc tagtccatca tgcgcccggc tgggatgaat ggtatcgcga
     4261 atgcacccgc cgcttcgccc atcatggcta tgcgacgatc tcgcccaacc tgtacttccg
     4321 cgatggccac ggcacggcgg aagacgtcgg cgccaaggtg cgcgccgccg gcggcatccc
     4381 cgacgagcag gtgctcggcg atctcgatgg cgccaacaaa ctgctgcgct ccatgcccta
     4441 tctcaacggc aaggttggca tcttcggtac ctgctccggc gggcgtcata gctttctcgc
     4501 cgcatgccgg accaagggca tcgacgcgct ggtcgaatgt tggggcggcg gcgtggtcat
     4561 gaaggcggac gaactgacgc cgaaaaaacc tgtggcgccg atcgactata ccaaagatct
     4621 ttcctgcccg atgatcggca tattcggcaa cgacgataaa tcgccgtcgc cggaacaagt
     4681 caatgcgcac gaggccgaac tgaagaaaca gggcaagaaa tacgagttcc accgctatga
     4741 cggcgccggc cacggctttt tctattacga tcgtccggct tatcgtcagg aacaggcggt
     4801 cgatggctgg aaaaaggtca tggccttctg cgaaaaaaac ttgaagtagg caacggagga
     4861 gcgattatgt gcacaagcat tacccatcaa gcaaaaatct atggccgcgg taaaagcggc
     4921 caagagtgga ttgagctcaa agaagccaac gtctcctacg atcatcccta tggcatgccg
     4981 ctcgaacacg cgctgaacat tgattttgtc gatgagtcca aaggacccgg cgcccgtgtc
     5041 gccgtcgaat tgagcgtcga cgcggcgcgt cgactggttc agaccatcca agccgtgctc
     5101 gcacaagccg agcatcaagg cgtcttggaa gacaaacacc aggcggccgt cggttaaccg
     5161 gcaaactaac tggggcggcg aagcccatgc ccgcttcgcc gcctcaacca ccttcgcaat
     5221 gcttctctac tcgcgctata gctgtcgata aattttccga ttcatcgatc tcgtcccatt
     5281 ggctgctgca gaagaaataa ctaaaagcgc cgaggccgcg ccgacgcgcc cctgggcttc
     5341 gctggcgatc cgggattacc gcctgatgtg gatcgccagc gtctgtaacg ccgtggcgat
     5401 ccaggtgcgc aatgtcgccg ggctctatca ggtctacgag atttccggat cggcactgca
     5461 attgggtctc accggctttt tccaagcgtt ccccttcgtt atcttcgggt tgttcgccgg
     5521 ggctgtcgcc gacgctttcg accgtaaaaa acttctcgtc gtcaccgtgg cgatccagct
     5581 gattccgagt ctattgctcg gcttcctgac catggccggg acgatccagg tctggcatat
     5641 tttcgctctg ggatttgtcg gcgcgctggt cgaagtgttc aattggccgg cccgttcggc
     5701 cttgatcccg cgcctggtgc cgccgtccta catgatgaac gcagtgaccc tcaacacgat
     5761 catcattcaa accagctttc tcttgggccc ggcgctgggc ggcctgctta tcgatcatag
     5821 cggcttggtc ttcacctatt ttctcaccat cgccatgttc atcccctccg gcatcgcgat
     5881 cttttccatg cgcacttccg gcgttcctga aggcgaacgg cgccgggtta acctgaaaag
     5941 catcttcgaa ggcatcgagt ttatctgggt gcaacgaata atcttatcgc tgtttctgct
     6001 cgacttcggc gtcaccttgg tgggatttta taaaccgatc ctgccgatct tcgccgccga
     6061 tgtatttaaa accggcgcct cgggcttggg catcctctac ggcgcgccgt cgctcggcgc
     6121 catcatcggc tcgatcgttt tattgatgat cggtaacgta aatcggatgg gagtcttggt
     6181 agtgtgcgcg gcgctgatct ttgccggcag cctgaccttg ctcggactgt ccaattcgtt
     6241 ttggctggcg gtgggcgcga cgttgatctt gggcttcacc gactcggtga gcgtcgccat
     6301 ccggcgcacg gtcgtgcaga tgatggcccc cgacggcatg ttgggccggg cgtcgagttt
     6361 gatcaccgcc tttgcccaag ccacgaatgg actcggcgcc gtcctcgccg gggccgccgc
     6421 ccagcaagtc ggcgcgccca acgcgctcct gctcggcagc gcgctgtgct tcgtcatgat
     6481 cctggccatc tgcctggcga tcccgcaact gtggcgctac cggtcttaag aagagcttgg
     6541 tcgcaaagct tgtcctaagc gcatgcgaag gaactgcgtc cgcatagcac tcaaagaacg
     6601 attgccggat tgaaggggcg gtttttaacc cgccgcgaca attctgcatt tcttttttat
     6661 gaactcttag gaaaatgccg actagaggca aaaatccccc ggtccttcgg cccgccccct
     6721 ttggcaaagg gggccattgg gggattctcc gtcgttgctt taaacgtggc gcagctcatt
     6781 tcatttgctg tgttgtaccg attggtacat gaattacttt gcgtcggttg tggctaaata
     6841 attcccgcgt ctttgagctc ttgcagcttc tctttggaca gtcccaactc accaccgaga
     6901 atcgcatcgt tgtcttcgcc cagtttgtgg ccaaggaagt ccaccgagcc tggggtggcg
     6961 gagaacttgg gcacgacgtt ttgcacgatc gcttcgccca aatccggatc gggcacgcgc
     7021 accatcgctt cgcgggcgcg atactggagg tcgcggaagg catcttccat gtcgaataca
     7081 gcagaatagg caacgccatg cttgtcgaaa tgctcgatga cttcgtctcg gctatgctgc
     7141 tcgatccagg cgccgacgac accgttgcac tcgatggagt tttccacccg cgccgaattg
     7201 tcgaggaact tgggatcggt ggtcatatct ttgcgaccga tcgcttcggc caggcgcaac
     7261 cagacgcttt gcgtgctcgc cgccagggtc agatatttgc catccttggt tttgaacatc
     7321 gagctcggcg ccacgtaggc gacgcggtta ccggtgcgct tatggacgat cttcatctga
     7381 tcgtactgga tcgggtcgaa gtcgaggaaa cggaacaccg cttcatagag cgccaagtcg
     7441 atcatctggc cttcgccgcc gcgcaggtca cggtggtaga gcgccatcat caccgacaac
     7501 gagccgaaga ttccggcgat caagtcgccc agtggatagc ccggcgacat cggcggcccg
     7561 tccggctcgc cgatcagatt ggtcaaaccg ctcatcgcct cggcgacccg gccaaagccg
     7621 gcgcgatttt tatacggccc ggtctggccg aaaccggaaa tgcgcagcat gatgatgcgc
     7681 ggatcgatgg cgcggatggc gtcgtagccg ataccccact tctcaaaggt gccggggcga
     7741 tagttctcga cgatgacatc ggtgcctttg accagctgtt tgaagatgtc cgcgccttcc
     7801 ggcttgccga gatcgagagt gatgcagcgc ttgttgcgcg ccaccgactt ccaccacagc
     7861 gggatgccat ccatgatcgg ctccaacttg cgctggccgt cgccgtattt cggatgttcg
     7921 attttaatca cat
//
LOCUS       DASQKS010000118         5814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_914686, whole genome shotgun sequence.
ACCESSION   DASQKS010000118 DASQKS010000000
VERSION     DASQKS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914686"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VE170_03080"
     CDS             <1..1637
                     /locus_tag="VE170_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ14443.1"
                     /translation="PSEATWAHLISTVLPEYTANTLVLLVLVAAGVVCCGVLSAWLVT
                     AYQFPGRGALEWALVLPMAIPAYVMAYAYTDWLQTAGPVQSMLRELTGWRVREYWFPE
                     IRSLPGAATMLSFALYPYVYLLARSAFLEQSRSSYEAAQLAGYGAWGRFWHVSLPLAR
                     TGIVAGVALALMETLADFGTVSYFAVNTFTAGIYRAWLSLGDSVAAGQLATCLLVFVL
                     AMLALERIHRGGARYTSNRAPISSQPLGGARALGAFTLCATPVAFGFVVPAAILLELA
                     LTDPQVQFGTRITGLITNTFILAGVAAMVAVIVAMLLAYAARTAKSPLVQTANRLAVL
                     GYALPGAVIAVGILLPLGKLDNALIAWLHEQFGVRTGLILTGSVTALIYAYLVRFLAV
                     AFQTVEAGLTRVTSSMDDAARSLGLSPGRALVRVHMPIMRSSVATAALLVFVDVLKEL
                     PATFAMRPFNFDTLAVEAYNLAKDERIAEAALPALLIVAIALLPLILLSRQIANSSRR
                     RRNDALGDPATALEPNRIRSHEVLFIAAPGKRTEAS"
     gene            1692..3665
                     /locus_tag="VE170_03085"
     CDS             1692..3665
                     /locus_tag="VE170_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HYJ14444.1"
                     /translation="MTSSCPIRSTPATFARLFLFGLIMLTATGALAAGDEAKRILALI
                     DYIGGDYRNAVQGGKVVSDAEYTEMSEFSARSLELFSQLKSAEGDKAGIEKDLRALAS
                     HIQQKTGEDAVPQLAQQIKDRLIKTYNIVTYPKALPNYADAKLIYLQNCAQCHGESGR
                     GDGPGRESMNPKTPPPANFTDADFMAGLSPFKVYNTTTFGIDNTAMASFAALPDQQRW
                     QVAFYVMALRFSAETAAAGESWLKDKPLPVTLAGVATLATSSDSELSDKIEKNFPNAP
                     TSDILAYLRRGLLEKKSSDPLLIARTLLGEAAEIYSKGDKEKAYQKAIEAYLDGFEMA
                     EPALFAKDAALGRSLEAQFTQFRNSIRQGVSAEEIQKQHGEIEAKLDQAAQTLASSDD
                     FSGYYAFLNSALIILREGLEAALILAAIIAMLRVMGAKESIRYIHLGWILAIAAGFLT
                     WLAAQTVLTFSGQHRESMEGFISIFAAAALFYVGYWLHTRTEAKRWQAFIQDKVQNVL
                     SSKSIFGLVSISFFAVYREAFEVVLFYQALWLQNENSHQAILGGFAAGVVALLAATIA
                     IFKLGLRIPLKYFFGVTGTLLYIMAFIFAGTGINQLQAAGWVPATPLDFPPAVPLLGI
                     YPTMETLAAQGLLVCIFIATSLWMAQERKKAMA"
     gene            4095..4307
                     /locus_tag="VE170_03090"
     CDS             4095..4307
                     /locus_tag="VE170_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14445.1"
                     /translation="MYGIKSSHGRWSLSLCNCSQDTAHFHYGNVVLHIARDDLRELGL
                     AMQSVAEDSEATDSENHIELKKGLVQ"
     gene            4310..4918
                     /locus_tag="VE170_03095"
     CDS             4310..4918
                     /locus_tag="VE170_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ14446.1"
                     /translation="MSAFIAIIIKGGIVMIPLLACSLISLALTIERFMFWAKLKTKDV
                     LDQILSLVEKGEFEQALNLGKTSSQPIARVLAAGIAHRNPAPAKAMEALAQAEIPMLK
                     SRLGVLDTIVTLAPLLGLLGTVVGMIGSFDVMSEAGMGQPHAITGGVAEALIATATGL
                     LIAILTLISYNYFTAKVEREMDRMEHFSSSLELLLHERAGKH"
     gene            4918..5331
                     /locus_tag="VE170_03100"
     CDS             4918..5331
                     /locus_tag="VE170_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ14447.1"
                     /translation="MRIQRRSIKKARIEIIPMIDTIFFLLVFFMISTLSMTQFKGMPV
                     NLPKAASGQQAPAESAAITIDKDGRLFLNQQAIDKAALGEALSQQLAKNAEMLVVINA
                     DDGVQHGQVVEVMDIARGANVAKMAIAVKPKEQKK"
     gene            5328..>5814
                     /locus_tag="VE170_03105"
     CDS             5328..>5814
                     /locus_tag="VE170_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14448.1"
                     /translation="MNDRWLVSSFTASSLIHLGIIPLAALVMHAKPIKPAMVPIELVD
                     VPRIEEPKKVEVPPPPPPPAPKPRLQKITAPKLISKPDFETSPLPPTGNTKEEIKEKP
                     VEQPLASLPDNSGAAKSGWNLGSKAVEAEGSAAGAGNLFGKGDVGVVGGSGVTGGGGG
                     TG"
BASE COUNT         1232 a         1680 c         1629 g         1273 t
ORIGIN      
        1 tgcccagtga ggctacctgg gcgcacctga tatcgacggt cctgcctgag tacaccgcca
       61 acactcttgt gctgctggtg ttggtcgctg cgggagtggt ttgctgtggc gtactctccg
      121 cttggttggt taccgcctac caattccctg gccgaggcgc actggaatgg gcgctggttt
      181 tgccgatggc gattccagcc tacgtcatgg cctatgctta caccgattgg ctgcaaaccg
      241 cagggccggt gcaaagtatg ctgcgcgaat tgaccggctg gcgcgtgcgc gagtactggt
      301 ttccggagat ccgttcattg cctggcgccg caaccatgct gtcgttcgcg ctctatccct
      361 atgtctacct gctggcgcgc agcgcgttcc tcgaacagtc gcgcagcagc tatgaggccg
      421 cccaactggc cggctatggc gcgtggggta gattttggca tgtgtcattg cccttggcgc
      481 gaaccggtat cgtcgccggc gtcgccttgg cactgatgga gaccctcgcc gatttcggca
      541 ccgtttccta ttttgccgtg aatactttta ccgccggcat ctaccgtgcc tggctgtccc
      601 taggcgattc agtcgcagcg ggccagctcg caacttgcct gctcgtgttc gtcctcgcaa
      661 tgctcgcgct cgagcgcata caccgtggcg gagcgcgtta taccagcaat cgcgcaccga
      721 taagctccca accgcttggc ggcgccagag cattgggcgc attcacgtta tgcgcgacgc
      781 cggtcgcatt cggtttcgtg gtaccggcgg cgatcctgct tgagttggct ctcaccgacc
      841 cgcaggttca atttggcacc cgcatcaccg gactgattac caacaccttt attttagccg
      901 gcgtcgccgc gatggttgcc gtcatcgtcg ccatgctgct ggcttatgcg gcacggaccg
      961 cgaagagtcc cctggtacaa acagcgaatc gcttggccgt gctcggctat gccctgcctg
     1021 gcgcggtgat cgcggtaggc attcttctgc ccctgggaaa gctcgataat gcgttaattg
     1081 cctggcttca cgaacagttt ggcgtgcgca ccgggctgat tctcaccggc tcggtcaccg
     1141 cgctcatcta tgcttatctg gtaaggtttc tcgccgtggc gtttcaaaca gtcgaggccg
     1201 gcctgacacg cgtgacatcg tctatggatg atgccgcacg cagcctgggt ctgtcacccg
     1261 gtcgcgcgtt ggtgcgcgtg cacatgccga tcatgcgcag cagcgttgcc actgcggcat
     1321 tactggtatt cgtcgacgta ttaaaagaat tgccggcgac ctttgccatg cggccattta
     1381 acttcgacac cctagccgtc gaggcctaca acctggccaa agacgagcgc atcgctgaag
     1441 ccgcactgcc ggctttgctc atagtcgcga tcgcgctttt accgctgatc ctattgtcgc
     1501 gccagatcgc caattccagc cggcggcggc gtaacgacgc gcttggggat ccggctacgg
     1561 cgcttgagcc gaaccgtatt cgcagccacg aggtactctt tatagcggct ccgggcaagc
     1621 gcacggaagc gagctaatac gttattggat agtattttga ctgagtaatt cgccttaaga
     1681 ataccagcac catgacttca tcctgcccca tcagatcaac accggcgacg ttcgctcggc
     1741 tcttcctctt cggtctgatc atgctaacgg cgaccggcgc attggccgcc ggtgatgaag
     1801 ccaaacgtat cctagcgctc atcgattaca tcggcggcga ctatcgcaac gccgtgcaag
     1861 gcgggaaagt cgtcagtgac gccgagtaca cggagatgag cgaattttcg gcacgcagcc
     1921 tggagctgtt cagccaattg aaatccgccg aaggcgataa agcgggtatc gaaaaagatc
     1981 tgcgcgcgct ggcaagccac atacagcaga aaaccggcga ggatgcagtg ccgcagttgg
     2041 cccagcagat caaggaccgc ttgatcaaaa cctataacat cgtcacctac cccaaggcac
     2101 tgcccaatta tgccgacgcg aaactgatct acttgcagaa ttgcgctcag tgccatggcg
     2161 agagcggcag aggcgacggt cccggccgcg agtcgatgaa tccgaagaca ccgccgccgg
     2221 ccaattttac cgacgcagac ttcatggccg gactctcgcc cttcaaagtc tacaatacga
     2281 ccacctttgg catcgacaac acggcgatgg cctccttcgc cgcgctgccg gaccagcaac
     2341 gctggcaagt ggcgttctat gtcatggcgc tgcgtttttc cgctgaaacc gcggctgcgg
     2401 gcgagtcctg gctgaaagac aagccgctgc cggtcaccct cgcgggtgtc gccaccttgg
     2461 caacttcgtc ggacagcgag ctatccgata aaatcgagaa gaattttcct aacgcgccga
     2521 ccagcgacat tctcgcctac ctcaggcgcg ggcttctcga gaaaaaatcg agcgatccgt
     2581 tgttgatcgc tcgcaccctg ctcggcgaag ccgctgagat atacagcaaa ggcgacaaag
     2641 aaaaagctta ccagaaagcc atcgaggcct accttgacgg cttcgagatg gccgagccgg
     2701 cgctgttcgc caaggatgcg gccttgggca ggtctctcga ggcccagttc acccagtttc
     2761 gcaattcgat tcggcaaggc gtttccgccg aggagattca aaaacaacac ggcgagatcg
     2821 aagccaagct cgaccaggcg gcacagacgc tggccagcag cgatgatttc tcaggttatt
     2881 acgcattctt gaattcggcg ctgatcattc tacgggaggg actagaggcg gcgctgattc
     2941 tcgccgcgat catcgccatg ctgcgggtga tgggcgcaaa agaatcgatt cgttatatcc
     3001 atttgggctg gatactggca attgccgccg gtttcctcac ctggctggcc gcccagacgg
     3061 tgctgacttt cagcggccag caccgtgaaa gcatggaagg attcatctcg atcttcgccg
     3121 cggcggcgct gttctacgtc ggctattggc tgcacacgcg gaccgaggcg aaaaggtggc
     3181 aggcgtttat tcaagacaaa gtccaaaacg ttctttcgag caaaagtatc tttgggctcg
     3241 tgagcatctc ttttttcgct gtgtatcgtg aggccttcga ggtcgtgctc ttctaccagg
     3301 cgctctggtt acagaatgaa aatagccacc aagccatcct tggcggattc gccgcaggcg
     3361 tagtcgcgct gctcgcggct accattgcta ttttcaagct cggtctgcgc ataccgctaa
     3421 agtatttctt cggcgtcacc ggcacactgc tctacatcat ggccttcatc ttcgccggca
     3481 ccggcatcaa tcaattgcag gcagccggct gggttccggc gacgccgctc gattttccgc
     3541 cggcggtgcc gttgttgggt atctatccga ccatggaaac tctggcagcc caggggctat
     3601 tggtctgcat atttatagct acctcgctat ggatggcgca ggaacgaaaa aaggcaatgg
     3661 cttaaatgcg tgtccaaggg caaggcgaac aggaaacggt ggcaacagtg actcaggatg
     3721 gcgatgcgct tcgttgccat tgcggcagct tggttgcccg actggtgccg aatggtgtgg
     3781 aaatcaaatg ccgacgctgc aaacggcgaa ttataattcc acttttgggc gttgctaaag
     3841 attaaataac gggaattcgt cgacgaatga tcgagctctg atagtttttc gaatattcgt
     3901 tatgtttaat tatccggtta cttaaagagc tattttagga ttgactaagt tttttattta
     3961 gtattgacat agtttttgga tttcgatata cgaacgtaac cagaggccag cgatccagaa
     4021 tccagagccc ggatttcgcc gaagatgtat cgacgagatt gcgggcattt ttatttttac
     4081 ggaggtcaat ctcaatgtac ggaatcaaat catcccacgg cagatggtcg ctctcgcttt
     4141 gcaactgtag tcaggacacc gcccattttc actacggcaa cgtcgtcctg catattgcgc
     4201 gcgacgacct gcgcgagctt ggtctcgcca tgcaaagcgt cgccgaagat agcgaagcta
     4261 ctgatagcga aaatcacatc gaactaaaga aaggtctggt ccaatagtca tgtcggcatt
     4321 tatcgcaatc atcatcaaag gcggcatcgt catgatcccg ctgctcgcgt gctccctgat
     4381 ctcactcgcg ctcaccatcg aacggtttat gttttgggcc aaactgaaaa ccaaagatgt
     4441 cttggatcaa atcttgtcat tggtcgaaaa gggcgaattc gaacaggctt taaaccttgg
     4501 caaaacttcc agccagccga tcgcgcgggt tttggcggca ggtatcgccc accgcaatcc
     4561 cgcgccggcg aaggcaatgg aagctctggc gcaggccgag atcccgatgc tcaaaagccg
     4621 ccttggagtt ctcgacacga tcgtgaccct tgcccctctg ctcggattgc tcggcaccgt
     4681 ggtcggcatg atcggttcgt ttgatgtcat gtcggaagcg ggcatggggc aaccccacgc
     4741 cattaccggc ggggtcgcgg aagcgctcat cgcgacggcc actggtttgt tgatcgcgat
     4801 cttgaccctg atctcctaca actacttcac ggccaaagtc gagcgcgaaa tggatcgcat
     4861 ggagcacttc agctcttcgc tggaattgtt gctgcatgaa cgtgcgggaa agcattaatg
     4921 cgcattcaac gccgcagtat aaaaaaagct cgtatcgaga tcattccgat gatcgacacg
     4981 attttttttc tactggtatt tttcatgatt tcgaccttgt cgatgaccca gttcaaaggc
     5041 atgccggtca atctgcccaa ggcggcgtcc ggccagcagg cgcctgccga gagcgcggcg
     5101 atcacgatcg ataaagacgg ccggttgttt ctcaaccagc aagcgatcga caaggcggct
     5161 ctgggtgaag ctttgagcca gcaacttgcg aagaatgccg aaatgttggt cgtcatcaac
     5221 gccgacgacg gcgttcaaca cggccaggtg gtggaagtca tggacatcgc ccgcggcgcc
     5281 aacgtggcaa agatggccat cgcagtaaaa ccgaaggagc agaaaaaatg aacgatcgct
     5341 ggctggtatc ttcattcact gcgtcgtcgt tgattcattt gggcataatt ccgttggctg
     5401 cgttggtgat gcacgccaag ccgattaaac ccgccatggt acccatcgag ctggtggatg
     5461 tgccgcgcat cgaagaaccg aagaaagtcg aagtcccacc ccctccgcct cccccggcgc
     5521 ccaaacccag gctgcaaaaa atcaccgcgc cgaagcttat atccaaaccg gatttcgaaa
     5581 ccagcccgct gccgccaacc ggcaacacca aggaagagat taaagagaaa ccggtcgaac
     5641 agccactcgc ttcattgccg gataactccg gcgccgccaa gtccggctgg aacctcgggt
     5701 ccaaggcggt cgaagccgaa ggcagcgctg ccggtgccgg caatcttttc ggcaagggcg
     5761 acgtgggcgt ggtcggtggt agcggcgtga ccggcggtgg cggcggcaca ggca
//
LOCUS       DASQKS010000119         7038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1321252, whole genome shotgun sequence.
ACCESSION   DASQKS010000119 DASQKS010000000
VERSION     DASQKS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321252"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VE170_03110"
     CDS             complement(<1..1073)
                     /locus_tag="VE170_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ14449.1"
                     /translation="MSYQVIGKSIPRVDNIGKVTGDARYTSDVWLPGTLWAKTLRSPH
                     AHARILSIDTTRAAKAPGVRAILTGNDVRGILYGRRYRDISVLAQDKVRFIGERVAAV
                     AADTLEQAEAALEAIEVQYEELPGVFDVVAAAQDGAPLVHPDMNSYKGLPKQFDKPTN
                     AFVQDVFTRGDVAVGFAQADVIVENTYTVPRVHQAFIEPHCCLVWIDEQERIQIWSPC
                     KVPQGLKSSMAAAFDLPKEKIRVNPVTIGGDFGGKGAPIDEPICYLLALRSGRPVKMV
                     MEYREEFIAGAPRHAGVMRLKTGTKRDGTIVAHEMEAFLDAGAYGGFRPGAVIGGVAH
                     AGGCYRAAHARIAVSRVYTNNIPG"
     gene            complement(1161..2141)
                     /locus_tag="VE170_03115"
     CDS             complement(1161..2141)
                     /locus_tag="VE170_03115"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ14450.1"
                     /translation="MMTDLAPPQIRGITGLELTAGLFSKCSDLLATQLKISLTSGKKD
                     AGPLPFSIGINGDVYGGMRAPLEVGMRRVDIAYVNPSAMVAMAYRGKGYYKQKMELRV
                     LGCFPSWDRIALVVSKDLAVKSIADIVRRKIPLHVSTRLSGVDNGTYFTIATILGFYG
                     FSFEQIKKWGGRVQECARPFAQERMRAIKQRSLTAIFDEGVSTEGGWLDLALANGYEI
                     IPIEPAIVKKLEALGYSRAMLPKKRYKQLKEDALTIDYSGWALVTHKWLPNRVAYAAI
                     ETIDDRKKVIPVDDDRPLNMKNLCHSTEKCPLRVPLHPGAVRYYKTKGYL"
     gene            complement(2156..3106)
                     /locus_tag="VE170_03120"
     CDS             complement(2156..3106)
                     /locus_tag="VE170_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14451.1"
                     /translation="MALATPFIVSAEKIRTVVPQSSVNYLSVYVAESRGLFHQEGFEH
                     ETLVLAGPVAAAALISGSVDFAGGGGSAMRAAAGGAPVKGIFFQTDKVTFYLVTDGSI
                     AKAADLKGKKIGVGGVGSSQDRLITAYVEQAGLSASDITRIAMGADAGRRILAIKSGT
                     INATMLDPGTVIYAEKEGLRTLAFLGDLFPYPFQAFAATDRKLRDNPEQVKRWLRAMV
                     GAVMFIRDNPEDAAEIALKRLRIGNVAKAALIDGIKRYNRAVPAGIPGVPSAEGLKNV
                     IDYEIKIPLKIDGELAQDKVLNLKLMQQVKIELESKKVSR"
     gene            3367..3642
                     /locus_tag="VE170_03125"
     CDS             3367..3642
                     /locus_tag="VE170_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14452.1"
                     /translation="MGTAAVLVGAKAPPLSTVQKQKAIHDKSRYKFAVLCEVIADLGF
                     DQETIGKITEVPRRIINEIASRRGYRAFTAEINELRESYRLHLRNFF"
     gene            4202..4696
                     /locus_tag="VE170_03130"
     CDS             4202..4696
                     /locus_tag="VE170_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14453.1"
                     /translation="MWNNFTSEALRTVGEDLDRRGIKTVGLRFEANLIVVEAGYQSPP
                     AATPVTLHYGPKDIEDFDRKARERNDYLSATLNFIYLSKILSSIGTYVGDKAARLFSL
                     SNIASTKTMPVIDIEYETVQGNRTFERLTDSDVYALCVRDHKRRGKRSALNDNRYSLF
                     SSLQ"
     gene            5594..6841
                     /locus_tag="VE170_03135"
     CDS             5594..6841
                     /locus_tag="VE170_03135"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HYJ14454.1"
                     /translation="MTTLTMKDEKRLDVIQRVYRSELTVGQAALVMGLSERQCYRIKA
                     RIGKAGAKGVLHGNRGRPCKRRLKDITVRRVLELARGKYQGFNDHHLTEKLKEQESIE
                     LSREKVRRILRAEGIVSPKKRRGLKHRSRRERRAAEGMMLQVDGSPHDWLQGRGPPMC
                     LIGAIDDATSKVTGGFFAQAESSWGYFTLFSDTFREYGLPHSIYTDCHSVFWTDREPT
                     LQEQLINHKPTTEVGRALEELGVTLILAHSPQAKGRIERLWNTFQDRLVSELRLAKAK
                     TLEQAQAVLERYIPVHNRKFAKPARAEPAWRKLSALQIQRALCFKQHRTVARDNTISF
                     EGTVLQIPKTSPFRSYANRRVEVHVLLDGAVEIFYKTEKIAAFDSKTTHTIGLYRSHG
                     KKEGFRYGPISMLTNLTQQTDLT"
BASE COUNT         1653 a         1954 c         1876 g         1555 t
ORIGIN      
        1 ccggggatat tgttggtgta gacccgcgaa accgcaatcc gggcatgggc ggcgcgatag
       61 cagccacccg catgagcgac gccgccaatc accgccccgg ggcgaaaacc gccataagcg
      121 ccggcatcga gaaacgcttc catctcgtgg gcgacgatgg tcccgtcgcg cttggtgccg
      181 gttttcagac gcatcacgcc ggcgtggcgc ggcgctccgg cgataaactc ttcgcgatat
      241 tccatcacca tcttcaccgg ccggccgctg cgcaaggcga gcagatagca aatcggctcg
      301 tcaataggcg cgcccttgcc gccaaaatcg ccgccgatgg tgaccgggtt gacgcgaatt
      361 ttttccttgg gaaggtcgaa ggccgccgcc atgctcgact tcaagccctg cggcaccttg
      421 cacggtgacc aaatctgaat gcgttcctgc tcgtcgatcc agacgaggca acaatggggc
      481 tcgataaatg cctgatgcac ccgcggcacg gtgtaggtat tttcgacgat gacatcggcc
      541 tgggcgaaac caaccgcgac gtcgccgcgg gtaaacacat cctggacaaa agcgttggtt
      601 ggtttgtcga actgtttggg caaccccttg tagctattca tatcggggtg gaccagcggc
      661 gcaccatcct gcgccgccgc gacaacatcg aacactcccg gcagctcttc gtactgcacc
      721 tcaatcgctt ccaatgccgc ttcggcctgt tccagagtat ccgccgccac tgccgccact
      781 cgctcaccga taaagcgcac cttgtcctgg gctaaaacgg aaatgtcgcg atagcgccgg
      841 ccatagagaa taccgcggac atcgttgccg gtcagaatcg cgcgaacccc cggcgccttt
      901 gcagcgcgcg tcgtgtcgat gctcaggata cgcgcatggg cgtggggact gcgcagtgtc
      961 ttcgcccata gggttcccgg caaccacaca tccgaggtat agcgcgcatc gccggtgact
     1021 tttccgatat tatcgacgcg tggaatggat tttccaatga cttgatagct cacgacggat
     1081 ctcctcctgg aatacgtaat cgagggatgg agtattggtt tcgggaaact cgatcactcc
     1141 cctgcgcgat catgccaatc ttacagatat cccttagtct tgtaatagcg caccgctccc
     1201 ggatggagcg gcacgcgcag cggacatttt tccgtgctat ggcagagatt tttcatgttg
     1261 agcggccggt cgtcgtcaac cggaatgacc ttttttcggt cgtcgatggt ttcgatggcc
     1321 gcgtaggcga cgcggttagg caaccatttg tgcgtcacca gcgcccagcc gctgtaatcg
     1381 atggtcaggg cgtcttcttt taattgcttg taacgcttct tcggcagcat ggcacggctg
     1441 tagcccaaag cttcgagctt tttgacgatc gccggctcga tgggaatgat ctcgtatccg
     1501 ttggccaacg ccagatccaa ccagccgcct tcggtgctga cgccttcgtc gaagatcgcc
     1561 gtcagcgagc gctgcttgat cgctctcatc cgctcctggg cgaatggccg ggcacattct
     1621 tggacgcgtc cgccccattt cttgatctgc tcgaaagaga atccgtagaa accgaggatc
     1681 gtcgcgatgg taaaataagt cccgttatcg acgcccgaca agcgggtcga aacgtgcaac
     1741 ggaatttttc gacggacgat gtcggcaatg gacttgacgg ccagatcctt ggacaccact
     1801 agagcgatgc ggtcccagga cggaaaacag cccagcacgc gcagctccat cttttgtttg
     1861 taatagcctt tgccgcgata ggccatcgcc accatggccg acgggttgac gtaagcaatg
     1921 tcgacccggc gcatgccgac ttccagcggc gcgcgcatgc cgccatagac atcgccgtta
     1981 atgccgatgc taaaaggcaa cgggccggca tctttcttac ccgaggttag cgaaatcttc
     2041 agctgcgtcg ccaacagatc ggaacatttg ctgaacaggc ctgcggtaag ctcaaggccg
     2101 gtaatgccgc gtatctgtgg cggcgccaga tcggtcatca taggaattca ccctttcatc
     2161 gcgagacttt tttactttcc agttcgattt tcacctgttg catgagtttc aaattcaata
     2221 ctttgtcttg ggccaattcg ccatcaatct tgagaggtat ttttatctcg taatcaatga
     2281 cgttcttgag accctcggca ctcggcactc cgggaattcc cgccggcacg gcgcgattgt
     2341 agcgtttgat gccatcgatc aaagccgcct tggcaacgtt gccgatgcgc agccgtttta
     2401 gcgcgatctc ggcggcatcc tccggattat cgcgaatgaa cattacagcg cccaccatgg
     2461 cgcgcagcca tcttttgact tgctccgggt tgtcgcgcaa cttgcgatcg gtggcggcga
     2521 aggcctgaaa cggataggga aacagatcgc cgagaaacgc cagggtgcgc aacccttcct
     2581 tttctgcgta aatcaccgtg cccggatcga gcatcgtcgc gttgatcgta ccgctcttga
     2641 tcgccagaat gcgccgtccg gcatccgcgc ccatagcgat gcgcgtgata tcgctggcgc
     2701 ttaaaccggc ttgttcgaca taagccgtga tgagccggtc ttggctcgat cccaccccgc
     2761 cgacgccgat ctttttgcct ttcagatccg ccgctttggc gatgctgcca tcggtgacta
     2821 aataaaaggt caccttgtcg gtctgaaaga aaatcccttt caccggcgca ccgcccgccg
     2881 ccgcgcgcat ggcgctgccg ccgccgccgg caaagtcgac actgccgcta atcaaggcgg
     2941 cagccgcgac cgggccggcc agcaccaagg tttcatgctc aaacccttct tgatgaaaca
     3001 ggccgcggct ttcggcaaca tataccgaaa gataattgac gctcgattgc ggcaccacgg
     3061 tgcgaatctt ttccgccgaa acaataaatg gagtggctaa cgccactaga agcgctaaga
     3121 ccggcaacgc ctgagaagaa atccatgcgc ggcacgcacg aagcagaaca atctttagta
     3181 aatgcctgtt gcatccctct tcgaaaaaga gcgacttagg gggaattaaa atctgacacg
     3241 acattcgcca agcattatca cacgaaagct aacgaggcga attgcaagaa ttctacggcc
     3301 ggtgtgtgca cttcggtgcc aatagcttcc aatggttttg gcgcagcgac gggacagttt
     3361 gggcacatgg gcacagccgc ggtactcgtg ggcgcgaagg ccccgcctct aagcacggtg
     3421 caaaaacaaa aagcgattca tgacaaaagc cgatacaaat tcgccgttct ctgtgaagtc
     3481 atagccgacc tgggctttga tcaagaaacc atcggcaaga ttaccgaagt accgagacgg
     3541 atcatcaacg agattgccag tcggcgcggc tatagagcgt ttacagccga gattaacgag
     3601 ctccgagagt cgtacaggct tcatctgcga aatttcttct agacgacgcc atggccctta
     3661 accttatggt catgaaccga tttgatgaat tgatattcgg ggcggacaaa tgacggcgat
     3721 aatcatcaac aatgtcatca tgaaggtaat gtcgatgacc cgtctggcgg aagccattta
     3781 gccatcgcgt cggcggcttc aaagtcaagg ttttcactct ggcgactagg ccaacttggc
     3841 cgcccatctc acaagtggcc cgagcccatt tcggctgcta tgtcacagtc acgtttttgc
     3901 atcgacatag actcgaactg tgagacttcg tgggctgctc ctcgtcgatc gcgccaacta
     3961 gctggtgctc ttacccgaat cgaattgatc ataccggtgt ccgatcggtc gaacctgtca
     4021 tgagtgacat ggccgtcagc acatggtgcg ggtcatatcg agtggcgaaa ttgaaaattg
     4081 ttaacttcag ggcacatctc tctgcgttcg cggcccaaaa accggccgac ccacccagta
     4141 aaggttggca ccacatctgc ttatgccaag cgtgtcagac tatcggttgg cgaggcgaca
     4201 tatgtggaac aattttactt cagaagctct acgcacggtc ggggaagacc tggaccgtcg
     4261 cggcatcaaa acggttggac tgagatttga agctaacctc atcgtcgtcg aagcaggtta
     4321 tcaatcgccc ccggcggcca ctccggtcac actgcattac ggtccgaagg atatcgaaga
     4381 ctttgatcga aaggcacgag agcgaaatga ttatctgtcc gcaacactaa actttatata
     4441 cttgtccaaa atattgtcgt cgataggaac ctatgtaggc gataaggcgg cgcgcttatt
     4501 tagtctctcc aatattgctt ctaccaaaac aatgcccgtc atcgatattg aatacgagac
     4561 ggttcaaggc aaccgaacgt ttgagcgtct cacggactcc gacgtttatg cactctgcgt
     4621 tagagaccac aagcgtagag gaaaaagatc agccttaaat gacaatcgat atagcctttt
     4681 cagctctttg caataaaggc ctcagtcttt gctgactgga tccccagagg aaaccgccaa
     4741 gccgtaaggc cgacaattct cattaacatc gataaggcga tagcgaaatt ttcggttcaa
     4801 ttagtccgct gcgggatatc cgaacactcc ttgtaattct ttttggctta gtttttcgcg
     4861 ctcttttcat tgcctttttc ctgatcggtc cgtaaaaaca aagctcagag ttctgccata
     4921 aacggcagtt ctcgagatcc ctacaaagga cacaaaagcc gcgctgaaga cacctcaatc
     4981 cgctgtagat agctgccgct ctgacgagat agatacgccg gaaatccatg aagtggccct
     5041 tcgatcaatt tcgctaggcg ctaaaggaaa agacccgtac aaattggaga gtcgagaaac
     5101 gctgccaaat atttggcgct taaatgttgg gccggcgcgg cgggtatggg acgagttttc
     5161 gtcgaaaggc caaaggaccg gcgaaggaat gcagcgcagt gtcggaagtg agtacctacc
     5221 cgtgagttag acccgaacag atggtctcat cgcgggtgcc gaatcatgct ggcggatacc
     5281 gcctggaact caatctgttt cctcatacaa agttttatat ctctccggta ataaagactc
     5341 atacagagcc gcctcaggaa tggagcggct tttttcaagg ggtcttcact tcaacttttt
     5401 ccgcgctatt ctaatgtcag tagttggcga ttctgctttt ggattgccac ccatttagca
     5461 gattagtagg tttgcctccc taccaccgac tcgaaaggcg cctcgatgac tccggggcgc
     5521 ctttctttat ggtgtagtag caacatgatt ctgacagggg ttaacggcaa cgtgattatg
     5581 tcagggtgga aggatgacaa ccctgacaat gaaagacgag aaacgactcg atgtaatcca
     5641 acgagtgtat cgcagcgaac tgacggtggg acaagccgcg ctggtgatgg gactaagcga
     5701 acggcaatgt taccggatca aagcccggat cggtaaggcc ggagccaagg gagtgcttca
     5761 cggtaaccga gggcggccgt gtaaacgcag gctcaaagac attaccgtaa gacgagtctt
     5821 ggaattagcc cgagggaaat accaaggatt taacgatcac catctgaccg agaagctcaa
     5881 agagcaagaa tcgatcgagc tgagccggga gaaagtccgg cgtatccttc gagctgaggg
     5941 gatcgtttca cctaagaagc ggcgcgggct caaacaccga agccgcagag aaagacgagc
     6001 cgctgaaggc atgatgctcc aggtcgatgg ttctccccat gattggctcc aagggcgggg
     6061 gccgccgatg tgtctgatcg gagccatcga cgatgctacc agcaaggtga cgggcggatt
     6121 ctttgcccaa gctgaaagct catggggtta cttcacgctg ttctctgaca cctttcggga
     6181 atacggctta ccccattcga tctataccga ctgtcactcg gtattctgga ccgatcggga
     6241 accgacgcta caggagcagt tgatcaatca taagcccact accgaagtgg gcagggcctt
     6301 ggaagaactc ggagtgactt tgatcctggc ccattcccct caagccaagg gtaggatcga
     6361 aagactgtgg aacacatttc aagaccggtt ggtcagtgag ttgcgattgg ccaaggccaa
     6421 gaccctggaa caggcccaag cggtcttgga gcgttacatc ccggtgcata accgtaagtt
     6481 cgccaagccg gctcgggctg agcctgcctg gagaaagctc agtgcgctgc agatccaacg
     6541 agctttgtgt ttcaagcaac accgtactgt ggcccgggac aacacgatta gttttgaagg
     6601 gacggtctta caaatcccca agacttctcc ctttcggtct tacgcaaacc gcagagtcga
     6661 ggtgcacgtg ttactcgatg gtgcagtgga gattttctac aagaccgaga agatcgcagc
     6721 cttcgactcc aaaacgaccc acaccattgg cttgtatcga tcgcacggta agaaggaggg
     6781 gttccgctat ggcccaattt ctatgctaac gaacttaacc caacagaccg acctgacata
     6841 atcgcgttgc tgttaacatg acatattgac gttgctacga cagcgatttc tctattcgaa
     6901 tcatcgtagc cacctccctg attttgaact gatgggcggg agtaccgcga aaccggccaa
     6961 actgtcaagc ggcaatttcg cgccatggtt ttttcattca aattgacacc cggcgggccc
     7021 tttgttattc gatggact
//
LOCUS       DASQKS010000120         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_60963, whole genome shotgun sequence.
ACCESSION   DASQKS010000120 DASQKS010000000
VERSION     DASQKS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60963"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VE170_03140"
     CDS             complement(<1..245)
                     /locus_tag="VE170_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14455.1"
                     /translation="MAPRIDLKDKVTGRAEYIEDLPDLPNTVYGATLLSPYSHARIIR
                     IDCAAALEMPGVLGVVDRDHLDGVNPRLKLAPHEHLK"
     gene            complement(464..2755)
                     /locus_tag="VE170_03145"
     CDS             complement(464..2755)
                     /locus_tag="VE170_03145"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ14456.1"
                     /translation="MFSVGSNVQRIDGIEKVAGKAVYTGDMRLPGMGYAKVLRSPVPH
                     ARIRRIDASRASGLPGVLAVLTRDTIKVAANSFGAYVRDQQIIATDKVRYAGDMMAAV
                     AATQPSIAAQALTLIDVDYEELPAVFSIDDALKAGAPLVHEKLERRDPGYGRGGSHIV
                     HDDSNICLHFRHQRGDIAAGFKDADAVFEDTFYFPSAQHYPMEPHVCVAHFEGETLTV
                     WSATQSPFPVRQELARIFALPFSGVRVIVPAVGGGYGAKSGIKTEGIAACLSRMCGRP
                     VRLAFGAEETFKTICQPRAKMTLKTGVKKDGTFVARHCEIFLDGGAYANSGPSVTEKA
                     GYRAHGPYRIPHVLTNAYSVYTNTVPGGAFRGFGGPQVAYAYETHTDMIAQRMAIDPF
                     DLRMKNLLDQGESFSPGDTPIDCDLKAGLKNIAQAIGWDSHGSQISYGPKRRGVGIAT
                     AMKDGGGTNKPANAAVKIFNDGSVLLSTGSVEVGQGMRTAFLQVVAEELSVPPAQVRV
                     AALDTHSTPFDKGTNASSATSIMGQAVLKAARDARRQLLEAAAAVLKAPAGDVRLENG
                     MALCGEERLSFREVMQRHFRESEGEIWGRGYFKIEKDQHVPLGFPSPFWEIGFGAAEV
                     EVDVETGAVKLLRYISLTDAGKMINPMQCEAQDEGAAVFGIGQALFEDLAYQDGQLLN
                     GTLIDYCLPRFTDLPAEFTTTIMEGGGGPGPYGAKGMGEGGILPVAPAIGNAVFAATG
                     TRIQSVPLTPDLVWRAMVKNGEG"
     gene            2907..3644
                     /locus_tag="VE170_03150"
     CDS             2907..3644
                     /locus_tag="VE170_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14457.1"
                     /translation="MADHIKMTPQEAKQKSEAIRKIILDTYHNEVKPSPFFTDLRAGT
                     LSQARLQGWIKNWYSFALEVNTGMATSYHQFVGFFKRHPDIENRIAQKIGEEFTTPAI
                     GGHARMLPVLGKALGVTPEQMIECELIPEARGLVDFFVRSWIDIPLAECYAVHMEEEI
                     FGFISRDFRQSLPKYGITNEDMKYFRVHEEADLDEDHGGTNQAILEVALQEGYGNERA
                     GWPLEYCGKIAVQMIGNFYDGVYRRYP"
     gene            3809..>4071
                     /locus_tag="VE170_03155"
     CDS             3809..>4071
                     /locus_tag="VE170_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ14458.1"
                     /translation="MTKFGVFLPVSGRAASRNTLRQAAQEAEALGYDSVWAADRLVIP
                     WKIGTTYPYSKEATFIVPPDRPFFDTMTCLAFLAGCTETIQLG"
BASE COUNT          826 a         1229 c         1142 g          874 t
ORIGIN      
        1 atttttaaat gctcatgggg tgccagcttc agacgcgggt tgaccccatc aagatggtcc
       61 cgatcgacga cgcccaacac tccgggcatt tcaagcgctg cggcacaatc gatacggata
      121 attcgcgcat gggaatatgg actcaatagc gtcgcgccgt atacggtgtt gggcaaatcg
      181 ggcagatctt cgatgtattc cgcccggcca gtgactttgt ctttcaagtc gatgcggggc
      241 gccaagggag tttccgattt tactccgcgg cgcggttttt cgattttaga ttccatcggg
      301 cgattcttta gatgttttgc tttgctatcc tttaaaatct gcgacggctg ccaattattg
      361 ccgccgggtg accacctctc caccacccag ggcgaatcat gattcgcccc tacatcggat
      421 tattttgcgc gcttctgcgt tctttgtggc taatctgccg gtctcacccc tcaccgttct
      481 taaccatcgc tcgccacact aaatccggcg tcagcggcac ggactgtata cgagtgccgg
      541 tcgcggcgaa gaccgcgttg ccgatggccg gtgccacagg aagaatgccg ccttcgccca
      601 ttccttttgc gccgtagggt cccggcccgc cgccgccttc catgatcgtg gtggtgaatt
      661 cggcgggcag atcggtgaaa cgcggcaggc aataatcgat cagggtgccg ttgagcagtt
      721 ggccatcttg ataggcgaga tcttcaaaca acgcttggcc gatgccgaac accgccgcgc
      781 cttcgtcctg ggcctcgcat tgcatcggat tgatcatctt gccggcgtcg gtgagcgaga
      841 tgtagcgcaa caacttcacg gcgccagtct caacgtccac ttcgacttcc gctgcgccaa
      901 aaccgatctc ccaaaacggc gacgggaaac cgaggggcac gtgctggtcc ttttcgatct
      961 tgaaataacc ccgcccccaa atctcccctt ccgattctct gaagtgacgc tgcatcactt
     1021 cgcgaaagct caaacgctct tcgccgcaca gcgccatgcc gttttccagt cgcacgtcgc
     1081 cggccggcgc cttcaatacg gcggccgcgg cttcgagcaa ttgccgccgc gcatcgcgcg
     1141 cggccttgag gaccgcttgg cccatgatcg atgtcgcgct gctggcgttg gtgcctttgt
     1201 cgaagggtgt ggagtgggta tccaacgcgg cgacgcgcac ctgggctggc ggcacggaca
     1261 actcttcggc gacgacttgc aggaacgccg tgcgcatccc ctgccccact tcgaccgaac
     1321 cggtcgacaa cagcacactg ccgtcgttga agatttttac cgccgcattg gccggcttgt
     1381 tggtcccgcc gccatctttc atcgccgtgg cgatgccgac gccgcgccgt ttgggtccat
     1441 aacttatttg actgccatgg gaatcccaac cgatcgcctg ggcgatgttt ttcaatccgg
     1501 ccttgagatc gcagtcgatg ggcgtatcgc cgggagaaaa gctttcgccc tggtccaaaa
     1561 gatttttcat gcgcaggtcg aagggatcga tcgccatgcg ctgggcgatc atgtcggtat
     1621 gggtttcata ggcataggct acctgcggtc cgccgaagcc gcgaaatgcg ccgccgggga
     1681 cggtgttggt atatacggag taggcattcg tcagcacatg gggaatccga tagggcccat
     1741 gggcgcggta gccggctttt tcggtcaccg atggaccgga gttggcgtag gcgccgccat
     1801 cgagaaaaat ctcacagtgg cgcgcgacga atgtgccgtc cttcttgacg ccggtcttga
     1861 gcgtcatctt ggcgcgcggc tggcaaatgg ttttgaaagt ttcctcggca ccgaacgcca
     1921 agcgtaccgg ccggccgcac atgcgcgaca agcaggcggc aatgccttcg gtcttgatgc
     1981 cgctcttggc gccgtagccg ccgcccaccg cgggcacgat gacgcgcacg ccgctaaatg
     2041 gcagagcgaa gattcgcgcc aactcttggc gcactggaaa ggggctctgg gttgccgacc
     2101 acaccgtcag cgtctcgccc tcaaaatggg cgacgcagac atggggttcc atcggataat
     2161 gctgcgcgct gggaaagtaa aaggtatctt caaagactgc atcggcgtct ttgaatccgg
     2221 cggcgatatc gccgcgctgg tggcgaaaat gcagacagat gttgctgtcg tcgtggacga
     2281 tatgcgaacc gccgcggcca tagccagggt ctcggcgctc gagcttttca tgcaccaacg
     2341 gcgcgcccgc cttgagcgcg tcgtcgatgc tgaataccgc cggcaactct tcataatcga
     2401 cgtcgataag tgttaatgcc tgtgccgcga tgctcggttg cgtcgccgcc accgccgcca
     2461 tcatgtcgcc ggcatagcgc actttgtcgg tggcgataat ctgctgatcg cgcacataag
     2521 cgccgaaaga attggccgcg accttgatgg tatcgcgcgt gagcacggcg agcacgcccg
     2581 gcaacccgct cgcccggctg gcatcgatcc tccgaatgcg cgcgtgggga accgggctgc
     2641 gcagcacttt ggcatatccc atccccggca gccgcatgtc gccggtgtag actgcttttc
     2701 cggcaacctt ttcaataccg tcgatgcgtt ggacgttgct tcccacggag aacatgacca
     2761 ggttccttat cttattaact tagcgttcca ccgttcaacc aactttcgct agggcgatcc
     2821 cagccgacct tgacgttgac aacaatcttg accttcggtt aagtggcctt gacgaatgaa
     2881 gtttcatgac gggaaaggaa ttaaccgtgg ctgaccatat caaaatgact ccgcaggaag
     2941 caaaacaaaa atccgaggcg atccgcaaaa tcatcctcga cacctatcac aatgaagtga
     3001 agccttcgcc gttcttcacc gacctgcgcg ccggtacgtt gtcgcaagct cggctccagg
     3061 gttggatcaa aaactggtat tccttcgcac tcgaggtcaa cacaggaatg gctacgtctt
     3121 accaccagtt cgtcggcttt tttaaacgcc atcccgatat agaaaatcgc atcgcccaga
     3181 agatcggcga agaattcact acccccgcca tcggcggcca cgcgcgcatg ctgccggtcc
     3241 tcggcaaagc cctgggcgtc acgccggaac aaatgatcga atgcgaactt atacccgaag
     3301 cgcgcggctt ggtagacttc ttcgtgcgtt cctggatcga tatcccgtta gccgaatgct
     3361 acgcggtgca tatggaagaa gagatcttcg gcttcatctc ccgcgacttc cgccagagtc
     3421 tgcccaagta cggcattacc aacgaggaca tgaagtattt tcgcgttcac gaagaagccg
     3481 accttgacga agatcacggc ggcaccaacc aagcgatcct cgaagtcgcc ctgcaagaag
     3541 gctacggtaa cgagcgcgcc ggctggccgc tggagtattg cggcaaaatc gccgtgcaga
     3601 tgatcggcaa cttttacgat ggcgtgtatc ggcgctatcc gtaggcgaga cggcagttcg
     3661 atccaatcaa ccgctgcgct cggttcaagc cgttcaaatc gtaattccga accgatccac
     3721 tccgacgtct tgaacgactt gaacgattcg accggttgca acgagatcga accgccttag
     3781 ctgtcaacga aatttgttcg gaggaattgt gaccaagttc ggagtatttc tgccagtctc
     3841 cggccgcgcc gccagccgca atacgctgcg acaagcggcg caagaagctg aagcgctggg
     3901 ctacgattct gtttgggccg cggatcgttt ggttattccg tggaaaatag gcacaaccta
     3961 tccctacagc aaggaagcga cgttcatcgt gccgccggac cggccgtttt tcgataccat
     4021 gacttgcctg gccttcctgg ccggctgcac agaaactatt caattgggca t
//
LOCUS       DASQKS010000121         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4681540, whole genome shotgun sequence.
ACCESSION   DASQKS010000121 DASQKS010000000
VERSION     DASQKS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4681540"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VE170_03160"
     CDS             complement(<1..775)
                     /locus_tag="VE170_03160"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14459.1"
                     /translation="MGFSFDTVADRLRNWFSQPGLILSSAIFCLSFFALDAGAQTAPQ
                     KITVSMSSTGMPSIQLFIAREKGFFREEGFDPQLIRMSANAAIAAGVAGDLDALGSVG
                     SAVRGIMRGVPLRVVAVDLRRPIFWLVSRPEFRNAKELKGKALGIATINGSQHSAARK
                     MLAIHGVDSDKELTFSQVGDEATQLQAMISNSIQVAGLTPPYVFIARDKFKMNVLESS
                     IDKFASIQQGIGVQTKYVQESPDWLRRLLRVKAKAARFFH"
     gene            complement(875..1660)
                     /locus_tag="VE170_03165"
     CDS             complement(875..1660)
                     /locus_tag="VE170_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HYJ14460.1"
                     /translation="MLAKLTLQLLLSLAKRTQGATLADLAADAGCTVAEVEAALSVLA
                     GVNLCENDPVFAHRYRLAERAAELGMSGFNIALRTRAWPYLQRLHDLTRQKTGLAVIE
                     GDFHRFIDECPHRPQSVLAALPPLRYAEPARLRAGSTAKAILASFPEPLAEQILARNA
                     DVVDPSNEAYVDVDAWQRAAPSRDELARVRRAGYASSRGERGRDTGTVSLSAPVVDRT
                     GSVCGVIWVGGTAPEREAPVAAALVQAAKELSAELAQVSSVSR"
     gene            1895..2860
                     /locus_tag="VE170_03170"
     CDS             1895..2860
                     /locus_tag="VE170_03170"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14461.1"
                     /translation="MCFAIVSGCICAPIAHGAGAVPDKVRAAYSSPSPTQGVLWVAQL
                     GGLFLRNGLSVEIIFTRAAIETLVAGEVEFAQMTGALMSSAKLQGADPVMIAGIQDQL
                     DDRLVARPTIKSMEDLKGKRIGIFRFGSASHLRVLNILPRYGLSERDVAFLQIGDNPA
                     RLIALLGGAIDATLLPPPDHLEALRGNMKLLLNLRELNVSYQGTGLVTTQRLLAKRRD
                     LARRFMKSYVEAIHLVKTNPEMSKRAFAKYRQTRDEKQLEDAYQTLREVVKPKPYPSL
                     EGFNTIIKDAIERIPAAKSANAKDFIDASLLEELDRSGYIDGLYR"
BASE COUNT          600 a          860 c          848 g          607 t
ORIGIN      
        1 cgtggaagaa gcgcgccgcc ttggccttga cccgcaataa ccggcgcaac caatcgggcg
       61 actcctggac gtacttggtt tgcaccccaa tgccctgctg gatactggcg aatttgtcga
      121 tggaactttc caagacgttc atcttgaact tgtcgcgggc gataaacacg tagggcggcg
      181 tcaagccggc gacctggatc gagttgctta tcatggcttg cagctgggtc gcttcatcgc
      241 ctacttgaga aaaggtcagc tctttgtccg agtcgacgcc atggatcgcg agcatttttc
      301 gtgctgctga gtgttggctg ccgttgatgg tcgcaatgcc aagcgccttg cctttcaatt
      361 ctttggcatt gcgaaactcc ggacgcgaca ccagccagaa aatcggccgg cgcagatcga
      421 cggcgacgac gcgcaagggc acgccgcgca tgatgccgcg caccgcgctg ccgaccgaac
      481 cgagggcgtc gagatcgccg gcgacgccgg cggcgatcgc cgcgttggcg ctcatgcgga
      541 tcaattgcgg atcgaagcct tcctcgcgaa agaagccttt ctcccgcgcg ataaaaagct
      601 ggatcgacgg catcccggtg ctcgacatgc tgacggtgat tttctgcggc gcagtctggg
      661 caccggcatc gagcgcgaaa aacgataggc aaaaaattgc cgagctaagg atcaatccgg
      721 gctgtgaaaa ccaatttctc agccgatccg ctacagtgtc gaaggaaaaa cccatggtga
      781 actttcctct ttgtcgtttg ggccgtgttg attctgaatc ttcataaaac cgatttctgc
      841 gctaattttc agtacgtgtc gtttcggcaa ccggttagcg cgaaaccgag gaaacctgcg
      901 ccaactccgc tgatagttct ttcgcggcct gcactagcgc ggccgccacc ggcgcctctc
      961 gctccggcgc ggttccgccg acccatatga cgccgcacac gctgccggtg cgatcgacga
     1021 ccggcgcaga caatgaaacc gtcccggtgt ctcggccccg ttcgccgcga ctcgaggcat
     1081 aacctgcccg gcggacgcgc gccagttcat cgcggctcgg cgccgctcgc tgccaggcgt
     1141 caacatcgac ataagcttca ttggacggat cgacgacatc agcattgcgg gccaagatct
     1201 gctcggccaa cggttccggg aaagaagcca gaatcgcttt ggcggtagag ccggcgcgca
     1261 atcgggccgg ctcggcgtag cgcagcggcg gcagcgctgc cagcacgctc tgcggccgat
     1321 gcggacattc gtcgatgaat cgatgaaagt ccccttcgat cacagccaaa ccggtctttt
     1381 gtcgggtcag atcatgcagt cgttgcaggt agggccaagc gcgcgtgcgc aaagcgatgt
     1441 tgaaaccgct catgcccagc tccgctgccc gctcagccaa tcggtagcga tgggcaaaga
     1501 ccggatcgtt ttcgcagaga ttgacaccgg caagaacact tagcgcggcc tcgacttccg
     1561 ccacggtgca accggcgtca gcggcgagat ccgcgagtgt tgctccctgg gtgcgcttgg
     1621 ccaggctgag caacagctgc aaggtcaatt tggcgagcaa gctatcggct tggctgttca
     1681 gacttcacct ctgaagcaat tatttatcgg cgcttaattg ctgcggtcgc cggcacgagc
     1741 attgggcata cccatcgcga catttggcag gagactatgc atgtcgaatc atcctgtcaa
     1801 ctggagtttg ctctggaaat ttggttgacg aggactgcgt atttgcctat tttccgacca
     1861 gatgaaatgg tttcgaccac cggtttggac cccgttgtgc tttgcgatcg tcagtggctg
     1921 tatttgtgca ccgatcgcgc atggtgccgg cgcggttccg gacaaagtcc gagccgctta
     1981 ttcctcgccg agcccgaccc aaggcgtgct ctgggtggcg cagttgggcg gactatttct
     2041 tcgcaacggc ctcagcgttg aaatcatctt cacacgcgcc gccatcgaaa cattggtcgc
     2101 cggggaagtc gaattcgccc aaatgaccgg cgcactgatg tcctctgcca agctgcaagg
     2161 cgccgatccc gtcatgatcg ccggcatcca ggatcagttg gacgatcggc tggtcgctcg
     2221 ccctacgata aaatcgatgg aagacctcaa gggcaaacgc atcggcattt ttcgctttgg
     2281 ctccgcgtcg catttgcgcg tgctgaatat tttgccgcgc tacggtttga gcgagcggga
     2341 cgtggccttt ttgcagatcg gcgacaatcc ggcgcgtctg atcgccttgc tgggcggtgc
     2401 catcgacgca acgctcttgc cgccgccgga ccatctggaa gcgttgcgcg gtaatatgaa
     2461 gctcttgctc aacctgcgcg aactgaacgt ttcttaccag ggcaccggtc tggtcaccac
     2521 ccagcgttta ctggccaagc gccgcgatct ggcccggcgc ttcatgaagt catacgtcga
     2581 ggcgatccat ctggtaaaaa ccaatccgga aatgtcgaaa cgcgcttttg ccaagtaccg
     2641 gcaaaccagg gatgaaaaac aattggagga cgcctatcag acactacggg aagtcgtgaa
     2701 gccaaagccg tacccgtctt tggagggatt caacactatc atcaaggatg ccatcgagag
     2761 aattccggcg gctaagagcg ccaatgcaaa agacttcatc gacgcgagcc tgctcgaaga
     2821 gttggatcgt tcaggatata tcgacggcct gtatcgttga aaggtctggc ggggccgccc
     2881 caatcaagtg cggattgagt ttgtcaccac caatt
//
LOCUS       DASQKS010000122         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3002262, whole genome shotgun sequence.
ACCESSION   DASQKS010000122 DASQKS010000000
VERSION     DASQKS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3002262"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VE170_03175"
     CDS             complement(<1..310)
                     /locus_tag="VE170_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14462.1"
                     /translation="MAAAESAKIIVNGLKDAGISIIVTLPDINLSDLLREVEETKDVI
                     HVPLCREEEGMGICAGGYLVGKKCAAIMQNGGFFNSNNAIVSTLLQYQIPVLLLIYYA
                     G"
     gene            457..1059
                     /locus_tag="VE170_03180"
     CDS             457..1059
                     /locus_tag="VE170_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ14463.1"
                     /translation="MIKLYDFKSSPNCQRVKVVLAEKNLPYEIVPVDLTKKEQKNADY
                     LKMNPYGKVPVLTDDNTVLYESLIINEYLEEKYPATSLLPKEPAKKAKARILTDYGMA
                     HFDSPYQKLRMELTKEAKEQSQPTIDGAKAELKKLLQRFEEELGDQQYLLGDFSLVDA
                     NLIPRFTRLEGFGILPDPSLPRLGKYMERMKARPSVKALL"
     gene            1094..3511
                     /locus_tag="VE170_03185"
     CDS             1094..3511
                     /locus_tag="VE170_03185"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYJ14464.1"
                     /translation="MEAPKSGLFATVFSALVLPVLNFFDKSSLPRYHGAVNLTGLRRE
                     VEVKFDRYAVPHVHAANQEDLFFAQGYLHAQERLWQMDMSRRFLSGRMAELFGDFPLP
                     WREFSTQFRGHKTSDLDFFIRLLGIRQAALQSMKLLSDGDLTRLNAYSQGINRYIEQC
                     GKKLPWEFRLLRSAPEPWTAEDSLTIGKGLAFLLSTALYTRLNLIPLAAKLQHDPEKL
                     RSLLPTYPADAPAISRAIVRQSTELCRFTNGMLADTDWHGAGHGSNNWVVGPEKSKSG
                     AAILSNDPHLRMTLPGMFYLMHLAAGEKSAADSYETWGGSMPGLPCIQLGQNRRIAWG
                     ITAALCDDAELYRERIHRIEGERYLAGHDWCKFGERRELIRIRGGKVIERIVRASRHG
                     PIMSDFANTDASGEVLALRWIASESSREIHSVYRLNQARDWREFLTALEFHSAPSLNL
                     LYADRSGNIGYALAGKLPKRGVEPSLLPLAGWDERNDWQGYIPFDELPRLYNPPGALI
                     ATANNKIVDSAYPNYLSNFFEPPQRIRRISQLLQSQERFSARDMAKLQLDQVSLHAVE
                     LIRTLSDDLRALPGDGRLLDDAVARLLAWDGNCAAAQVPAAIFHVFHQRLLANLLSDE
                     LGGELFAAYTEILNQCIVPTDKIFGNERSCWFARRPRRALVAQSLREACAELRAAFGP
                     DCERWHWGKLHQLSLNHGLGRLAVLRRAINIGPMPSGGDGMTVNVGFYRHASPYSQTV
                     GAALRYVIDFDDPEYSGYVLTSGQSGHPGSRHYRDQFAVWQSGAKIPLSADCGASDDH
                     CLVMKPC"
BASE COUNT          827 a         1066 c          940 g          794 t
ORIGIN      
        1 cgccggcata atagatcaac agcaacaccg gaatttgata ctgcaataac gtgctgacga
       61 tggcgttgtt gctgttgaaa aagccgccgt tctgcatgat cgcggcgcat tttttgccga
      121 ccaaatagcc gccggcacaa atacccatcc cctcttcttc ccggcagagc ggcacatgaa
      181 tcacgtcttt ggtttcttcc acttcgcgca ataggtcgga tagattgata tcgggcaagg
      241 taacgatgat gctgatgccc gcgtccttta agccgttgac gattattttt gccgattccg
      301 cagcagccat cgagaaaaac tcctgtattg cgtcgcgccg ttctccgctt aacatcaatg
      361 gtagagtact tcaagtaaac gcgctggctg agacactttt cctttggccg gtttcctagt
      421 ataagtgcca cgtgcccata cgaaaggagc cttaccatga tcaagcttta cgatttcaaa
      481 tcgtcgccca actgccaacg ggtcaaagtc gttttggcgg agaaaaatct gccctacgaa
      541 attgtcccgg tggatctgac gaaaaaagaa cagaaaaacg ccgactatct taaaatgaac
      601 ccctacggca aagtgccggt gctcaccgac gataacacgg tgctgtatga gtcgctgatc
      661 atcaacgaat acttggagga aaaatatcct gcgacctcgc tcttgcccaa ggagccagcc
      721 aagaaagcca aggcacgcat tctcaccgac tacggcatgg cccatttcga tagcccttat
      781 caaaaattgc gcatggagct aaccaaagaa gccaaagaac agagccaacc gaccatcgac
      841 ggcgccaagg cggaattgaa aaaacttttg cagcgtttcg aagaggagct gggcgaccaa
      901 caatacctgc ttggcgactt ctccctggtc gatgccaact tgatcccgcg cttcacccgc
      961 ctggaaggtt ttggcatcct gcccgatccg tcgctgccgc gcctaggaaa atacatggaa
     1021 cgaatgaaag cgcgtccttc ggtcaaagca ctgctgtgaa aacgagaaga ttgcccaaga
     1081 tctagaacgc cacatggaag cgccgaaaag cggtttgttc gcgaccgtct tttcggcgct
     1141 tgtgcttcca gtcctcaatt ttttcgacaa gtcttccctg ccacgctacc acggcgcggt
     1201 caatctcacc ggactgcgtc gagaagttga agtcaaattc gatcgctatg cggtgccgca
     1261 cgtccacgcc gccaaccaag aagatttgtt cttcgcccaa ggttacctgc atgcccaaga
     1321 gcgcctctgg caaatggaca tgagccggcg ttttctcagc ggacggatgg ctgagctatt
     1381 cggcgatttc ccgttgccct ggcgtgagtt ctccactcaa tttcgcggtc acaagactag
     1441 cgatctcgat ttcttcatcc gcctgttggg gattcgtcaa gcggcgctgc aatccatgaa
     1501 gctactttca gacggcgacc tgacccggct taacgcctat agccaaggaa tcaatcgtta
     1561 catcgagcaa tgcggtaaaa aacttccctg ggaatttcgc ctgttgcgct ctgctcccga
     1621 gccatggacc gccgaagaca gtttgaccat cggtaaggga ttggcgttcc tgctttccac
     1681 cgcgctctac acgaggctca acctgattcc gctggcggca aagctgcagc atgatccgga
     1741 gaaactacgg tcgttactgc caacctatcc cgcagacgcg ccggccatca gtcgcgcaat
     1801 agttcggcag agcaccgagc tttgtcgctt taccaatggc atgctcgccg acaccgattg
     1861 gcatggcgcc ggtcatggaa gcaacaactg ggttgtcggt ccagagaaat cgaaatccgg
     1921 cgctgccatc ctatccaacg atccgcattt gcgcatgaca ctgcctggga tgttttatct
     1981 catgcacctt gcggccgggg aaaaaagcgc agcagatagc tacgaaacct ggggcgggtc
     2041 tatgcctggg ctgccttgca ttcaattggg gcagaatcgc cgaatcgcct ggggcatcac
     2101 cgcggcgctg tgtgacgatg ccgagcttta tcgtgagcgg attcatcgaa tcgagggtga
     2161 gcgttatctt gccgggcatg attggtgcaa gttcggcgaa cggcgtgagt tgatacggat
     2221 ccgcggcggc aaagtcatcg aacggattgt ccgtgcaagc cgtcacggac ccatcatgag
     2281 cgatttcgcc aacaccgacg cgtcgggaga agtattggca ctgcgctgga tcgccagtga
     2341 atcaagccgg gaaattcatt cggtctatcg gctcaatcaa gcgcgtgact ggcgggaatt
     2401 cttgaccgcc ctggaattcc acagcgcgcc cagcttgaat ctcctctacg ctgaccgtag
     2461 cggcaatatc ggttatgccc tcgctggaaa acttcccaaa cgcggcgtcg agccctcgtt
     2521 actgccgctc gccggttggg atgaacgcaa cgactggcag ggttatattc ctttcgacga
     2581 actgccgcgg ctgtacaatc ctccgggcgc attaatcgcc accgccaaca acaaaatcgt
     2641 cgattcggct taccccaatt atctttccaa ctttttcgaa ccgccgcaac gcattcgccg
     2701 gatcagccaa ctcttgcagt ctcaggagcg gtttagcgct agggatatgg ccaagctcca
     2761 gcttgaccag gtttcactcc atgccgtgga gttgattcga acgctcagtg acgacctgcg
     2821 ggcgcttccc ggcgacggcc gtttactcga cgacgccgtg gccaggttgc tcgcctggga
     2881 tggcaactgc gccgcggcgc aggtcccggc agcgatcttt catgtgttcc atcagcgcct
     2941 gcttgccaat ctgttaagcg acgaactcgg cggcgaatta ttcgccgcct acaccgagat
     3001 tctcaatcag tgcatcgtgc ccaccgacaa gatcttcggc aatgagaggt cttgctggtt
     3061 tgccaggcgc ccgcgtagag ccttggttgc ccaatcatta cgcgaagcct gcgccgaact
     3121 acgtgctgcc ttcggcccag attgtgaacg ctggcactgg ggcaagctcc atcaactatc
     3181 gcttaaccac ggcttgggac gcctagccgt gttgcggcgg gcaatcaaca tcggtccaat
     3241 gccctcgggc ggcgacggca tgaccgtcaa cgttggtttc taccgccacg ccagtcccta
     3301 ttctcagacc gtcggcgccg cactgcgtta tgtcatcgac tttgacgatc cagaatactc
     3361 aggctacgtc ctaacatccg ggcaatccgg tcaccccggg tcgcgccatt accgcgatca
     3421 atttgccgtt tggcaaagcg gcgcaaagat cccgctttca gcggactgcg gcgcatccga
     3481 cgaccattgt ttagtaatga agccatgcta aggtatgcgg catcgaatga atcaatcaac
     3541 tgcgccatca agccgaaccg ccaactcagg caagcatatg ccgatcaatc tattgatcgc
     3601 cgatgacgaa agactatttc gtcagag
//
LOCUS       DASQKS010000123         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2594980, whole genome shotgun sequence.
ACCESSION   DASQKS010000123 DASQKS010000000
VERSION     DASQKS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594980"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VE170_03190"
     CDS             <1..348
                     /locus_tag="VE170_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HYJ14465.1"
                     /translation="HINRAPVAGRVNDVRYQSGKFFAAYKEDASRVNEQNALRLVDER
                     ERNWGVVQIAGAVARRIVCRAKAGDMLGRGERFGLIMFGSRTDIYLPQGCRIEAVEGQ
                     RVKGGVTIIGRFS"
     gene            345..1205
                     /gene="pssA"
                     /locus_tag="VE170_03195"
     CDS             345..1205
                     /gene="pssA"
                     /locus_tag="VE170_03195"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HYJ14466.1"
                     /translation="MSAMNAQEPEAPQEKDKLRLLRRRRDRPRMTLRQRMPAEKLRKG
                     VYLIPSLFTAGNLMCGFFSIIATFRGEYVNAALLILLANAFDGVDGYIARLTRTTSQF
                     GVEFDSLADVVAFGVAPAVLVYIWALVPWQNWGWLAAAFYVVCGAARLSRFNVQAQGP
                     AKNHFVGLPIPAAAQMISSSVLLYYHIGFEGAPTRHVTFLLVIFGLAILMVSSIPFFS
                     LKNNDMRKRHPEWMFLSGVILITLIIAEWHIMFFAIVLLYTLSGPLLWCLTKLKRRRE
                     SRRGTAQALP"
     gene            1306..2871
                     /locus_tag="VE170_03200"
     CDS             1306..2871
                     /locus_tag="VE170_03200"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYJ14467.1"
                     /translation="MDQMANDNIVQIFDTTLRDGEQSPGASMTVEQKVVIARQLEKLG
                     VDVIEAGFAASSEGDFESVRRVAQEVSQPRVVSLARAQEGDINRALKAVEGAKKPGIH
                     TFIATSDIHLKHKLRMTREEVVEAAVKAVSYAKQFTDYVEFSAEDASRSDPEFLLQIF
                     REVIRAGAKTINVPDTTGYAIPSEFGALIANLIERTAGGDRVIWSAHCHNDLGLAVAN
                     SLAAVHNGARQIECTINGIGERAGNTSLEEVVMALRTRKNYLNLDTNIRSEQLFPSSR
                     LLSQVTGIAIPINKPIVGDNAFAHEAGIHQDGVLKHKQTYEIMTPESIGIPGNKLVMG
                     KHSGRHAFDERLKHLGFNLSKEDMNRAFVRFKELSDKKKNVYDEDIEAVVAEEVLRMP
                     GRPDRFELLYMNVNSSTDGVPTATVKMRVGEAEKMDHAAGDGVVDACYKVITKITDSQ
                     SQLVRYSVNALTGGADAQGEVACLIEDAGIRVSGQGSHTDIIMASALAYINALNKLEE
                     RKRYRRVVEKEGP"
     gene            2868..3962
                     /gene="leuB"
                     /locus_tag="VE170_03205"
     CDS             2868..3962
                     /gene="leuB"
                     /locus_tag="VE170_03205"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HYJ14468.1"
                     /translation="MNYKIAVLPGDGIGPEVMGEGTQVLNQVAQLYGFTATLEPGIVG
                     GASIDAHGKPLTDGVLNLAKQSDAVLLGAMGGPKWDGLDYAIRPERALLALRQELGLF
                     ANLRPVKLFSALASASTLKREVVEGTDMLVVRELTGGIYFGEPKGVTMLPDGTERGVN
                     TEVYTTPEIERIAHVAFQAALKRRKKVTSVDKANVLEVTELWRKVVTRIHKKEEGYGG
                     IELEHILVDNCAMQLIRNPRQFDVVVTTNMFGDILSDEAAMLTGSIGMLPSASIGGKV
                     GMYEPVHGSAPDIMGQDKANPLATILSVAMMLRYSFDQGAAAARIEKAVEEVLDEGYR
                     TADIQEPGCKLLGCKQMGRLVRDKIAAAKG"
     gene            4029..>4390
                     /locus_tag="VE170_03210"
     CDS             4029..>4390
                     /locus_tag="VE170_03210"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162781.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYJ14469.1"
                     /translation="MREVLDERQFPVGEVRLLASERSVGQFLPFQSRQLAVEVLKADS
                     FKDIDIALFSAGGSVSSEFAPMAVAAGAVVIDNTSVFRMEPDIPLVVPEVNAKEIANY
                     KSRGIIANPNCSTIQMVVA"
BASE COUNT          933 a         1169 c         1367 g          921 t
ORIGIN      
        1 catatcaatc gggctccggt tgccgggcgg gttaacgacg tgcgctatca aagcggcaaa
       61 tttttcgcgg cctataaaga agacgcctcc cgcgtcaacg agcagaacgc gctgcgtctc
      121 gtcgacgaac gggagcgcaa ctggggcgtg gttcaaatcg ccggcgcggt cgcccggcgc
      181 attgtctgcc gcgcgaaagc cggggatatg ttgggccgtg gcgaacgctt cggtttgatc
      241 atgttcggct cgcgcaccga tatttattta cctcaagggt gccgtatcga agcggtggaa
      301 ggacagcggg ttaaaggcgg agtgacgatt atcggaagat tctcatgagc gcaatgaatg
      361 ctcaagagcc cgaggcgccg caggaaaagg acaagctgcg cttgctacgc cgtcggcgcg
      421 accggccgcg catgaccttg cgtcaaagaa tgccggcgga gaaactgcgc aagggcgttt
      481 atctgatccc cagtctgttt accgcgggca atttgatgtg cgggtttttt tcgatcatcg
      541 cgacttttcg gggtgagtat gtcaacgcgg ctttactgat tcttctcgcc aacgcgttcg
      601 atggcgtcga cggctatatc gcgcgcttga cccgaaccac cagccaattt ggcgtcgaat
      661 tcgattccct cgccgacgtt gtcgctttcg gtgtcgcccc ggcggtcttg gtatacatct
      721 gggcactggt gccatggcaa aattggggtt ggctggcggc ggcattctac gtcgtgtgtg
      781 gcgcggcgcg cctgtcgcgc ttcaatgtcc aggcccaagg accggcgaag aaccatttcg
      841 tcggtctgcc gattcccgcc gccgcgcaaa tgattagctc atcggttctt ttgtattatc
      901 atatcggttt cgagggggcg ccgacgcgcc atgtgacgtt tctcttggtg atttttggct
      961 tggcgatttt gatggttagc agtattccat tcttcagttt gaagaataac gacatgagaa
     1021 aacgccaccc ggaatggatg tttctttccg gcgtgatttt gattacactg atcattgccg
     1081 agtggcatat tatgtttttc gccatagtct tactgtacac gttatccggg cccttattat
     1141 ggtgcttaac caagcttaag cgtcgacggg agtcgagacg ggggacggcg caagcattgc
     1201 cgtgaattcc ggctcgagta aaggcgaaac aaactcccgt tggccctgcc gcgggagttt
     1261 tcttttcggg gtgggccagc ggacaacggt agaggagaca ttcaaatgga ccagatggcc
     1321 aacgataata tagtgcaaat tttcgatacg acgctgcgcg acggcgagca atcgccgggg
     1381 gcgagcatga ccgtggaaca aaaggttgtg attgcgcgcc agttggagaa gctcggcgtc
     1441 gatgttattg aagcgggctt cgcggcatcc tcggagggcg acttcgaatc ggtgcggcga
     1501 gtcgcgcagg aggtcagcca gccacgggtg gttagtttgg cgcgcgccca ggagggcgat
     1561 attaatcgag ctctcaaagc ggtggagggg gccaagaagc cgggaattca tactttcata
     1621 gcgacctccg atattcatct caagcataag ctgcgcatga ctcgtgaaga ggtggttgaa
     1681 gcggcggtca aggcggtgag ttacgccaag cagtttaccg attatgtcga gttctcggcc
     1741 gaggacgcgt cgcgtagcga cccggagttt ttgctgcaaa tcttccgcga ggtgatccgc
     1801 gccggcgcca agacgatcaa tgtcccggat accaccgggt atgcgattcc gtcggaattc
     1861 ggcgctttga ttgccaacct gatcgagcgt accgccggcg gcgaccgggt gatctggagc
     1921 gcccactgtc ataacgattt gggcctggcg gtggccaatt ctctggccgc ggtgcacaac
     1981 ggcgcgcgac agatcgaatg cacgatcaac ggtatcggcg agcgcgccgg caatacttct
     2041 ctcgaggaag tggtcatggc tctgcgcacg cgcaagaatt atctcaatct cgacaccaac
     2101 attcgcagtg agcagctttt tcccagctcc cgtttgctgt cgcaagtaac cggcattgcg
     2161 attccaatca acaaaccgat cgtcggcgat aatgccttcg cccatgaggc cggcattcat
     2221 caagacggcg tgctcaagca caagcagacc tacgagatca tgacgccgga gtccattggc
     2281 attcccggga ataagctggt catgggcaaa cattccgggc gtcatgcctt cgatgaacgg
     2341 ttgaagcatc tcggctttaa cttgagcaag gaagatatga atcgcgcctt cgtacgcttc
     2401 aaagagttgt ccgataagaa gaagaacgtt tatgacgagg atatcgaagc cgtcgttgcc
     2461 gaggaagttt tgcgcatgcc ggggcggccg gataggttcg agctgctcta catgaacgtc
     2521 aactcgagca ccgatggggt gccgacggcg acggtcaaga tgcgcgttgg cgaggcggaa
     2581 aagatggatc atgccgcggg cgacggcgtg gtcgacgcct gttacaaagt gatcaccaaa
     2641 atcaccgatt cgcagtcgca gttggtgcgc tactcggtca atgcgcttac cggcggcgcc
     2701 gatgcccagg gagaagtcgc ctgcttgata gaagacgccg gcatccgcgt ctcgggccag
     2761 ggctcgcaca ccgacatcat catggcgagc gcgctcgcgt atatcaacgc gctcaacaaa
     2821 ttggaagaac gcaaacgcta tcggcgggtg gtggaaaaag agggtccatg aactataaaa
     2881 tcgcggtttt acccggagac ggcatcggcc ctgaggtgat gggcgaaggc acgcaggtac
     2941 tcaatcaagt ggcgcagctt tacggcttca ccgccactct ggagcccggc atcgtcggcg
     3001 gcgcgtccat cgacgcccac ggcaagccgc tcaccgacgg ggtgttgaat ctcgccaagc
     3061 agagcgacgc ggttttgctt ggcgccatgg gcggccccaa atgggatggg ctcgactatg
     3121 cgatccgtcc ggaacgtgcg ctcttggcgt tgcgccaaga gttgggctta tttgctaatc
     3181 tacgcccggt taaattattt tccgcgctgg cgtcggcgtc gacgctcaag cgggaggtgg
     3241 tcgagggcac cgatatgctg gtggtgcgcg agctcaccgg cgggatttat tttggcgaac
     3301 ccaagggcgt gacgatgctg cccgacggca ccgagcgcgg cgtcaatacc gaagtctaca
     3361 cgacgccgga gatcgaacgc atcgcccatg tggcgtttca ggcggcgctc aagcgacgca
     3421 agaaagtgac gtcggtcgac aaggccaatg tgctcgaggt taccgaactt tggcgcaagg
     3481 tggtgacacg cattcacaaa aaagaggagg gctacggcgg tatcgagttg gaacatatcc
     3541 tggtcgacaa ctgcgccatg caattgatcc gcaatccccg gcagttcgat gtcgtcgtca
     3601 cgaccaatat gttcggcgat attttaagcg acgaagcggc gatgctcacc ggctccatcg
     3661 gtatgctgcc gtcggcaagc attggcggaa aagtcggtat gtacgaaccg gtgcatggca
     3721 gcgccccaga tatcatgggt caggacaaag ctaatccgct ggcgacgata ctcagcgtcg
     3781 cgatgatgtt gcgttactcg tttgaccagg gcgcggcggc ggcgcgcatc gaaaaggcgg
     3841 tcgaagaagt tctcgacgaa ggctaccgca cagcggatat tcaagagccc ggttgtaagc
     3901 tgttgggctg taagcagatg ggccggctgg tgcgcgacaa gatcgcggca gcgaagggat
     3961 aggtttttgt gaagaaagaa aagtacaacg tcgcgatcgt tggcgccacc ggcgccgtcg
     4021 gcgagcaaat gcgcgaagtc ctcgacgaac gccaatttcc ggttggggag gtgcgcttgt
     4081 tggcctccga gcgatccgtc gggcagttcc taccgtttca aagccggcag ttggccgtcg
     4141 aggtactcaa ggccgactcg ttcaaggaca tcgatatcgc cctgttctca gccggcggca
     4201 gtgtcagcag cgaattcgcg ccgatggcgg tggcagccgg cgcggtggtg atcgacaata
     4261 cttcggtgtt tcgcatggag ccggatattc ccttggtggt tccagaggtc aatgccaagg
     4321 aaattgccaa ctacaaatcc cgcggtatca tagccaatcc caactgctca accattcaaa
     4381 tggtcgtagc
//
LOCUS       DASQKS010000124         4556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5524355, whole genome shotgun sequence.
ACCESSION   DASQKS010000124 DASQKS010000000
VERSION     DASQKS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5524355"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VE170_03215"
     CDS             <1..1074
                     /locus_tag="VE170_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14470.1"
                     /translation="AGFAADARPAGQANWEQTVAAAKKEGKLNFYVGRYGSEPLLNEF
                     RKEFPEIKLVTVNGAGNSLGTRIITEIRAGHVVADLFSGGANTNYEVLYEGKALDSIK
                     SLLLLPEVLDETKWFEGRHRYTDPEQRHVFIYIANPSSSGFYYNTNMVNPKEIKSYWD
                     LVNPKWKGKYVSQDPLSTGLGASLQFYYYHPELGAEYIKKLFGDMQPVYGRDRRQITD
                     WLAQGRYAMCVGCRDTARAKSQGLPVDELDNIDWKEGVQLTSGGGSMSFIKGAPNPNA
                     AKVFVNWFLSRRGQIALQKHQDLYGEDAPNSRRIDIPKDVLVPVNRMQAGKRYFDVSD
                     PKYADMTPMFDLLKQIVKVKEGK"
     gene            1181..1471
                     /locus_tag="VE170_03220"
     CDS             1181..1471
                     /locus_tag="VE170_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14471.1"
                     /translation="MPTQIHIYTINRGSLDEFATEWRDKIRPLREKFGFQVSGGWKVK
                     ETNQFVWFLSRDSAEDWDTQNQGFYQSEERRLLQPDPARHIARVEQYFVESV"
     gene            complement(1609..3834)
                     /locus_tag="VE170_03225"
     CDS             complement(1609..3834)
                     /locus_tag="VE170_03225"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ14472.1"
                     /translation="MIYRSVNRAVPRIEGADKVSGNMRYAADLNFPAALAAKILRTSL
                     PHARILSIDTSKAAKLPGVRGVITGADVKGIMVGLRMKDMPLLALEKVRYAGEPVAAV
                     AADTDEIAEEAVNLIDVQYQELPFITDPVEATEAGAPVLHDDPMSYKNAPERDIDLPN
                     VQSYGKWTNGNVDAGFQQAARIFEHTFRTPLGFHGYIEPHACTVQIHDDGQIEIWASN
                     KAPFTLRTRFARDVGVEEAKVKVHILPVGGDFGGKTSVAEAPICYFLAKKTGKAVRMV
                     LEYTEEVTAISHRHPAVITLRTGVDGDNKICAMAIKAVFSGGGYAALKANAEVTVQGP
                     RRAASYYRFPAIQVETLCAYTNQVPCTQTRTPGSPQTTFAVESQIDIIARELKLDPVK
                     FRMDNLLNAGEPTPFGQKLQGIVVKETLTKALEASGWNKPKAKNVGRGVAVYERPSGA
                     GKSGAAITVEADGSLSVHLGVPDVGPGIHTVVQQIVSEVLDLPRDRVSVRVGDTDSSP
                     YDSGTGGSKSTNSVGTAAYQAVTEVKEKLIALTATKFGCKREAIAESNGRYNVPGQKP
                     MSCTDMMRHAVAQNGGALTHLSNYEPARAPITSFAAQVAEVEVDPVTGQVKVTKLTTV
                     HDSGTVLNHLSYTGQIDGGIINGLGFALMEDNSLVEGKMPTVNLGEFKMATIADVPKL
                     TTVLMESPAGPIPYQGKAIAEIPNVPTAAAIANAIHDACGARIFDLPITAEKVYAVLR
                     K"
     gene            complement(3890..4102)
                     /locus_tag="VE170_03230"
     CDS             complement(3890..4102)
                     /locus_tag="VE170_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14473.1"
                     /translation="MSDEKKDDYVVSFRGAKLSRFHANRVGFTIICGFVAAILLVPTI
                     GDDSPLLTKFLVIGIAILAFTLFRRK"
     gene            complement(4112..>4556)
                     /locus_tag="VE170_03235"
     CDS             complement(4112..>4556)
                     /locus_tag="VE170_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14474.1"
                     /translation="RRLEIIPANYQINNYLEEFVAMQLYMHSKIFQTFPKLKIVICHC
                     GGGLNRFIPNDHHVGQGDFSKNLFFDSCVYAVNYLEAGIKQRGVDQVLFATESPGSGG
                     AIRKDTGKPSDDMIPVIDGLGFLSAEDKLKIFQKNPLKVFTKVKI"
BASE COUNT          957 a         1230 c         1323 g         1046 t
ORIGIN      
        1 gcgggttttg ccgccgacgc caggccggcg gggcaagcca actgggagca gaccgtcgcc
       61 gcggcaaaga aggaaggcaa gttgaacttt tacgtcggcc gctacggcag tgaaccgttg
      121 ctgaacgagt ttcgtaaaga gtttcccgag atcaagctgg taaccgtcaa cggcgcaggc
      181 aattcgctgg gaaccaggat tattaccgag attcgcgccg gccatgtggt cgccgatctt
      241 ttcagcggcg gcgctaatac caactacgaa gtcctgtacg aaggtaaggc gctcgactcg
      301 atcaaatcat tgttgctcct tcccgaggtg ctcgatgaga ccaaatggtt tgaagggcgc
      361 catcgctaca ctgaccccga gcagcgccat gtgtttattt acatcgccaa cccgtcgtcg
      421 tcaggattct actacaacac caacatggtg aacccgaagg agatcaaatc ctactgggat
      481 ctggtcaatc caaaatggaa gggaaaatac gtttcccagg atccgctgag cacgggactc
      541 ggcgcttcgc tgcaattcta ttactaccac cccgaacttg gcgccgaata tattaaaaag
      601 ctatttggcg atatgcagcc ggtctatggg cgcgaccgcc ggcagatcac cgattggctg
      661 gcgcaagggc gctacgccat gtgcgtcggt tgccgagata cggcccgggc caagtcgcaa
      721 ggattgccgg tcgacgaact cgacaacatc gactggaaag agggcgtgca gctaacctcc
      781 ggcggcggct cgatgagttt tatcaagggc gcgcccaatc ccaacgcggc caaggtcttc
      841 gtcaactggt ttctctcgcg ccgcggccag atcgccctgc aaaaacacca ggatctctac
      901 ggcgaagatg cccccaactc gcgccgcatc gatattccca aagacgtctt ggtgccggtc
      961 aaccgcatgc aagccggcaa acggtatttc gacgtctccg acccaaagta tgccgatatg
     1021 acaccgatgt tcgatctgct caagcaaatt gtgaaggtaa aagagggaaa gtagcagact
     1081 cggattgttt ttagtttccg gactggaatc tgcgccgagt tagaggttcg ggctcgcgat
     1141 gaaggtgaca gcccacgcca ataggctttc tcataaactt atgccgacac agattcacat
     1201 ctacaccatc aatcgcggca gtctcgatga gtttgccacc gagtggcgag acaagatcag
     1261 accgttacga gagaagtttg gttttcaagt gagcggcggc tggaaggtga aagagaccaa
     1321 ccaattcgtc tggtttctga gccgcgatag cgcggaagat tgggatactc agaatcaagg
     1381 gttttatcag tccgaggaac ggcgtttatt gcagcccgat cctgcgcggc atattgcccg
     1441 ggtcgaacaa tattttgtcg aatcggtgta gctcggttgc aaaaaccaga agagcagctc
     1501 ccgccgtgaa ttgcaccgtt ggctaggttt aagagggcgg gttacaaaca tcgctcaaca
     1561 acgcgcattt ttttcgtgcg ttgtttgcgt ccttcacaga ttcccaagtt atttccgcag
     1621 caccgcgtaa accttttccg ccgttatcgg taaatcaaaa atgcgcgcgc cgcaggcgtc
     1681 gtggatcgcg ttggcaatgg ccgcggcagt tgggacgttg ggaatttccg ctatggcttt
     1741 gccttgatag ggaatcggtc cggccgggct ttccattagc accgtcgtaa gttttggcac
     1801 gtcagcgatg gtggccatct tgaattcgcc gaggttgact gtgggcatct tgccctcgac
     1861 tagggaatta tcttccatga gggcgaagcc caagccatta ataatgccgc cgtcgatctg
     1921 accggtgtag ctcaggtgat ttaacaccgt gccagagtca tgaaccgtcg tgagtttcgt
     1981 gactttaacc tgtccggtaa cgggatcgac ttcaacttcc gcgacctgcg cggcgaatga
     2041 ggtgatcggc gcgcgcgccg gttcgtagtt actcaaatgc gtcagcgcgc cgccgttctg
     2101 cgcgacggcg tggcgcatca tgtcggtgca agacatcggc ttttgcccgg gcacgttata
     2161 gcggccattc gattcagcga ttgcttcgcg cttacagcca aacttcgttg cggtgagagc
     2221 gatgagtttt tccttaactt ccgtgacggc ttgataagcg gcggtgccga cgctgttggt
     2281 cgacttgctg ccgccggtgc cggagtcgta gggcgagctg tcggtgtcgc cgacccgaac
     2341 gctgacgcga tcgcggggta ggtcgagcac ttcgctgacg atctgctgga ccaccgtatg
     2401 gatgcctgga cccacgtcgg gcacgcctag atggacgctc aggctgccgt cggcttcgac
     2461 ggtgatagcc gctccggatt ttcccgcgcc cgatggccgc tcatagacgg cgacaccgcg
     2521 tccgacattc ttcgcctttg gcttattcca gccggatgct tccagggctt tggtcagggt
     2581 ttctttgacg acgatgcctt gaagtttttg cccgaagggg gtcggctcgc cggcgttgag
     2641 cagattgtcc atgcgaaatt tcaccggatc aagtttcaac tcccgggcga taatgtcgat
     2701 ctgcgattcc actgcgaagg tagtctgtgg gctgcccggc gtgcgcgtct gggtgcaggg
     2761 aacttggttg gtgtaagcgc atagagtttc gacttggatc gctggaaagc gataatagct
     2821 cgccgcccgt cgagggccct ggacggtgac ttccgcgttg gcttttagcg cggcataacc
     2881 gccgccgcta aaaaccgcct tgatagccat agcgcaaatc ttgttgtcgc cgtcgacgcc
     2941 agtgcgcagc gtaatcaccg ccggatggcg atgtgagatt gcggtgactt cctcggtgta
     3001 ttccaagacc atgcgcaccg ctttgccggt tttcttagcg agaaaatagc agatcggcgc
     3061 ctcggctacg gatgtcttgc cgccgaaatc gccgccgacg ggaagaatgt gaaccttgac
     3121 tttcgcttcc tcgacgccga catcgcgggc aaaacgagta cgcagtgtga aaggagcttt
     3181 gttcgacgcc cagatctcga tctggccgtc atcgtgaatc tgcaccgtgc aggcgtgggg
     3241 ctcgatgtag ccgtgaaatc ccaacggcgt gcgaaaggta tgttcgaaaa tccgcgccgc
     3301 ctgctgaaag ccggcgtcga cgttgccgtt ggtccattta ccgtaggact gcacgttggg
     3361 caggtcgatg tcgcgctcgg gtgcgttctt gtaggacatc ggatcatcgt gcagcaccgg
     3421 ggctcctgct tcggttgctt ctaccggatc ggtgataaag ggcagttctt gatactgcac
     3481 gtcgatgaga ttcacagcct cttcagcgat ctcgtcagtg tcggccgcga ccgcagccac
     3541 cggctcgccg gcataacgta ccttctcaag ggcgagcagg ggcatgtctt tcatgcgcag
     3601 gccgaccatg atgcctttga catcggcgcc ggtgatgacg ccacgcaccc caggcagctt
     3661 cgccgctttg ctagtgtcta tgctaaggat gcgcgcgtgg ggcagggatg tacgtaatat
     3721 cttcgccgcc aacgcggccg gaaagtttag atccgccgcg tagcgcatgt tgccggagac
     3781 tttgtccgct ccctcgatgc ggggaactgc tctgttgaca ctcctataga tcattcatgt
     3841 ctccgtaatt ggtagtaggt ttagacgcgt tgtttggcta ctccgtgatt tacttccttc
     3901 ggaatagtgt aaaagcaaga atggcaatgc caatcaccag gaatttggtg agtaacggac
     3961 tatcgtcgcc gatcgtgggc acaagaagga ttgcggccac aaagccacag atgattgtga
     4021 agccaacccg gttagcgtgg aatcggctga gttttgcgcc cctgaacgag acaacgtagt
     4081 catctttctt ttcatcgctc atcgctatgc gtcatatttt cactttggta aacaccttca
     4141 acgggttctt ctggaatatt ttcaacttat cctcggcgct taaaaacccc aggccatcga
     4201 ttaccgggat catatcatcg gacggtttgc cggtgtcttt gcggatggcg ccgccggagc
     4261 cgggggattc ggtggcgaag agaacctggt cgacgccgcg ttgcttgatg ccggcttcca
     4321 gataattgac agcgtacacg caggagtcaa agaacagatt cttgctgaaa tcaccttggc
     4381 cgacatggtg gtcgttggga ataaagcggt tcaagccgcc gccgcagtgg cagatgacga
     4441 tcttcaattt cgggaaggtc tgaaaaatct tgctgtgcat gtagagctgc atggcgacga
     4501 actcttcgag atagttgtta atctgataat tcgcggggat gatttctaac cgccgg
//
LOCUS       DASQKS010000125         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3005742, whole genome shotgun sequence.
ACCESSION   DASQKS010000125 DASQKS010000000
VERSION     DASQKS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005742"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VE170_03240"
     CDS             complement(<1..594)
                     /locus_tag="VE170_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYJ14475.1"
                     /translation="MANNLDKLRELNDKAEAGGGADRARRQHEQGKLLARERLDILLD
                     SGSFIEIDRLRTHHSTDFGLDKQKFLGDAVVTGYGAIDGRLVYVYSQDFTVFGGSLSR
                     VVADKICKVMDMAIKNGAPLIGINDSGGARIQEGVDSLAGYGDIFQRNVMASGVVPQL
                     TAIMGPCAGGAVYSPAITDFIFMVKTNSYMFITGPDVI"
     gene            796..1989
                     /locus_tag="VE170_03245"
     CDS             796..1989
                     /locus_tag="VE170_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ14476.1"
                     /translation="MFEAKAKPQTLSSGSAPQPIQLKPNQAILLYAASEGDANMLYAT
                     GFFVPDPFIFFQHKNHRHIVMSDLEIDRAKKQAHADRVLSLSLYQKKLRKLGKSPAAV
                     IDIVDMIFRERAIRSLIVPANFSALLLDQLRAKGYSVQIKRDPFWPQRETKNNLEVKQ
                     ITESLRIARLGLEAGIRMLKRTKTKRDGYLYLHGTRLTSEMLKTAVNTTIMAQGWLPS
                     HTIISSGNQCVDPHHEGTGPIRANTSIIFDIFPRSQHNGYFGDLSRTVVRGRASEKLK
                     AIYATVQAGQQIGYAQIRDGVNGKEVHQNILDMFAARGFPTGRINGRMQGFFHGTGHG
                     LGLDIHEPPRIAPIDATLRTGHVVTVEPGLYYIGVGGVRLEDVVVVKPKGNRNLTDCP
                     QFLEI"
     gene            2021..2725
                     /locus_tag="VE170_03250"
     CDS             2021..2725
                     /locus_tag="VE170_03250"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYJ14477.1"
                     /translation="MATEPREIADLIAALDHPDKPTIRAAVDKLIALAETSETVRQAL
                     HNRLSEADHGNYWPVAYVLGHQAQPSAVVIRTLLGALDHREPDIRWANGLLLVRIAQQ
                     VGAVVPLLTELCATGSANQKRMALYCLRDLALNDEQSRAAMLKSLEDPEPTVRVAAAT
                     SLKVRTDVDAAVRQQLLATYLHDADDRVKNVVAVTLASLGSPSGEFMRALNEASRSGN
                     GQIKKSADAALKMLQK"
     gene            complement(3435..3821)
                     /locus_tag="VE170_03255"
     CDS             complement(3435..3821)
                     /locus_tag="VE170_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14478.1"
                     /translation="MHVSCVKPCELDALTYKRSDPLIIHWREAKPFQITIGVDPQSIP
                     DVAEGWHHPITREDAPIWDLPAISQLVNGQHPGRTREDAITCFCNNVGLGLQFAAVGS
                     EVLARAREARVGREIPTDWFLEDVHP"
     gene            complement(3855..4514)
                     /locus_tag="VE170_03260"
     CDS             complement(3855..4514)
                     /locus_tag="VE170_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14479.1"
                     /translation="MLILSNDEIEKILPVGACLDVLEDAYRDLGNGMAATVPRYDVFS
                     PTKSSNEFYEYKTMSGVLPNRGIAALRLNSSVVKWYEKAGGIRKDKLPVAGGDRYVGL
                     IMLFSTETGEPLAIFPDGYVQKLRVAGASAIAARYLARKNSTRMALLGSGWQASAHLV
                     TMAMVRDLKQVRVFSPTPESRRRFVDEWRGQVNAELIESASAAEAIKDADMVVCTTNS
                     I"
     gene            4664..5713
                     /locus_tag="VE170_03265"
     CDS             4664..5713
                     /locus_tag="VE170_03265"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14480.1"
                     /translation="MSLHLNAKTVRRFSFIGFLVAAIFAAPFARANPYLAKPGEAPVT
                     VYVATCAVTGGFIQLYAALDQNLFEKYGIAIKHVVIRGGTNINLAALGADEVQFLYCA
                     ADSTLPGMAVGRDAILVASPLVGLPYVIIARKEIRTVQDLKGKSIGVGSVAGLPYRLL
                     RIFVKKFGLNDTQIRPVGGSQPERYNAMMQGVIDSGPFTPPMDARGKKDGFNLIYHMN
                     DLGLPAIYSSLHTNAKSLRERRQTVQRFVAALAEAVAFVENNPEKAKASVAKILRVND
                     EEALQSSYDAYAKKHVNRRLIVPLDAVADSVEVARDAGAKVSKKANEIVDNSFAENLQ
                     KSGFLKELWGGKLPN"
     gene            5730..>6399
                     /locus_tag="VE170_03270"
     CDS             5730..>6399
                     /locus_tag="VE170_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14481.1"
                     /translation="MLQPSGLVSGHYECRSFAESLPILKEFLALSVVDEKADEKILQH
                     PNTGWQLVVHANGSAAPIKSMRNHYGVRVATNAEVDTATEYLESRKNQFGIKVIKPRE
                     NHNAYSVHFYEPGGNYWEIESYEDRHKAGLPFDVSFPWKTPLTEEQFPGRGYVPQAFT
                     HGTIECNDWASSVNFYKQGLGMDVITHIDTPKPHNIKHPAKPWYVVSLEVPEKNRKYL
                     GLMQR"
BASE COUNT         1473 a         1888 c         1724 g         1314 t
ORIGIN      
        1 gatcacgtcc ggaccggtga tgaacatgta actgttggtc ttgaccatga agataaaatc
       61 ggtgatcgcc ggcgaataga ccgcgccgcc ggcgcagggt cccatgatcg cggtgagctg
      121 cggcactact cccgacgcca tcacgttgcg ttgaaatata tcgccatagc cggcgaggct
      181 gtcgacgccc tcttgaatgc gcgcgccgcc ggagtcgttg atgccgatta gcggcgcgcc
      241 gttcttgatc gccatgtcca tgactttaca gattttgtcg gcaaccacgc ggctcaggct
      301 gccgccaaaa acagtgaagt cctgtgagta gacgtaaacg agtcggccgt cgatggcgcc
      361 gtagccggtg accacggcgt cgcccaaaaa tttttgttta tccaggccga agtcggtgct
      421 gtgatgcgtg cgcaggcggt cgatctcgat gaaactgccg ctgtcgagca gaatgtcgag
      481 acgctcgcgc gccaaaagtt tgccttgttc atgttggcgc cgggcgcgat cggcgccgcc
      541 gccagcttcg gctttgtcgt tgagttcgcg gagtttgtcg agattattag ccatcgtcgt
      601 cgagagaaat tggttaggac gcgaagtcga aaatagtttc gccagcgaat cgcggcatca
      661 taacaaaagc ccttggactg tcaaccgaat gaagcgaatt cacgaggaaa aacttgcagt
      721 tccagagcta agttgttagc gtgaacgaac tctcggaaaa ccataaacga cgcgaatcga
      781 ggcgatcaag caatcatgtt cgaagccaag gcaaagccac aaaccctatc gagcggctcg
      841 gcgccgcaac cgatccaact caaaccgaac caggcaatcc tgctttacgc cgccagcgaa
      901 ggcgacgcca acatgcttta cgccaccggt ttcttcgtcc ccgatccgtt tattttcttt
      961 cagcacaaaa accatcgcca catcgtcatg agcgatctgg agatcgaccg cgccaagaag
     1021 caagcccatg ccgatcgggt tctgtcattg tcgttgtatc aaaagaagct gcgcaagctc
     1081 ggcaagtcgc cggccgcggt catcgacatc gtcgacatga tatttcgcga acgcgccatt
     1141 cgctcgctga tcgtgccggc gaatttttcc gcgctcctgt tggatcagct gcgcgccaag
     1201 ggctacagcg tgcaaatcaa acgcgacccg ttctggcccc agcgcgagac taagaataat
     1261 ctcgaggtaa aacaaatcac cgaatccctg cgtatcgccc ggctgggctt ggaagcgggc
     1321 atccgcatgc tcaaacggac caagacaaaa cgcgacggct atctttatct ccacggcacg
     1381 cggctcacat ccgaaatgct caagaccgcg gtcaacacga cgatcatggc gcaaggttgg
     1441 ctgccgagcc acacgatcat ctcgtcgggg aaccaatgcg tcgatccgca ccacgaaggc
     1501 acgggcccga ttcgcgccaa cacctcgatc atcttcgaca tcttcccgcg ttcccagcac
     1561 aatggttatt tcggcgactt gagccgcacc gtagtgcgcg gccgggcgtc ggaaaaacta
     1621 aaggctatct acgccacggt ccaagccggg cagcagatcg gctacgcgca gatccgcgat
     1681 ggcgtcaacg gcaaggaagt ccaccaaaat attctcgaca tgttcgccgc ccgcggcttt
     1741 ccgactggga gaattaacgg ccgcatgcag ggctttttcc acggcaccgg ccatggtttg
     1801 ggtttggaca ttcacgagcc gccgcgcatc gcgccgatag acgcaactct gcgcaccggc
     1861 catgttgtca cggtcgaacc gggactctat tacatcggcg tcggcggcgt cagactggaa
     1921 gatgtcgtcg tggtcaagcc caagggtaac cgcaatttga ccgactgccc gcagttttta
     1981 gaaatctaaa ccggctatat tcccgcgttg caattcgctc atggcaaccg aaccacgcga
     2041 gattgccgat ctcatcgccg ccctcgatca tcccgacaag ccgacgatcc gcgccgccgt
     2101 cgacaaacta atcgcattgg cggagacttc cgaaactgtt agacaagcgc ttcataatcg
     2161 cctgagcgaa gcggatcacg gaaactattg gccggtcgcc tacgttctcg gccatcaagc
     2221 ccagccttcg gcagtggtga ttcgcacgct gctcggcgcc ctcgatcacc gcgaaccgga
     2281 tattcgctgg gctaacggcc ttctgcttgt ccgcatcgct caacaggtcg gcgccgtggt
     2341 ccctttgctg accgagctgt gtgcaaccgg cagcgccaac cagaaacgca tggcgcttta
     2401 ctgcctgcgt gatttagcgc tgaacgacga gcaaagccgc gccgccatgt tgaaatccct
     2461 ggaagatccg gaaccgacgg tgcgggtcgc cgccgcaacc agtctgaaag taagaaccga
     2521 tgtcgatgct gccgtccggc aacagctgct agcgacttat ttgcatgatg cagatgaccg
     2581 cgtgaagaat gtggtcgccg taaccttggc cagtttaggg tcgccaagcg gagaatttat
     2641 gcgggcgttg aatgaagcga gccggagcgg caatgggcag atcaaaaaaa gcgccgatgc
     2701 agcacttaag atgctgcaaa aataaaaagg cccgctacac tcggcagctt gcgcgaccgg
     2761 tgcagcgggc cactgcggtg gctgagggag attctcaggc tctaggcccg aatctccatt
     2821 cagagtcgcc gcctagagcc taagtctcag ccacctcaca gccagtactt aaatcaatca
     2881 tgtctggacc acctcctttc cctgctgaca attagtttac gaaagtgttt tttctctgtc
     2941 aataagataa tagagcgatg gaaaaaaata atttgattta acgttacact cccgtcgtgc
     3001 aaccgcgcac gcaggctgtg tgtgcatccg tcgaattatc aacggcttga agttgctcag
     3061 tcgaaaacgc cagtggaacc gaaagctcga ctgcgcccta gggaaagtct tgaaaattaa
     3121 aagacaatcg ccaatgcggc cgctcgatca tagagatcgt gaaacagctt gagattccat
     3181 gcacagtgtg actttttctg taaatttact ttacagtttc tgtgcggaat taacttagag
     3241 ttgagcgctt atctcggctc cgaaagagcc gcggcgcagc agctaatact tttttgttgt
     3301 gtgcagcttc gacaatcgtt aggtcaagtg tctgcatctc tggcaattag cttgcgcgga
     3361 aggatattcg cggtgcttga tcgttattaa atagatctgg cgttgaactt tgcggtttgc
     3421 atctcgacga aattctacgg atgcacatct tccaaaaacc aatccgtcgg aatctcccga
     3481 cctactcgcg cctcacgcgc gcgtgccaat acttcactgc cgacggcggc gaactgcaaa
     3541 ccaaggccga cgttattgca aaagcacgtg atggcatctt ctcgagtgcg tccggggtgc
     3601 tggccgttga ccaactgcga aatcgccggc aaatcccaga tcggcgcgtc ttcgcgggtg
     3661 atcggatggt gccaaccctc agcgacatcg ggaatcgact gcggatcgac gccgatggtg
     3721 atttgaaacg gtttcgcttc gcgccagtga ataatcagcg gatcggagcg cttgtaagtc
     3781 aaagcgtcga gctcgcacgg cttgacgcat gagacgtgca ttcccggctc caagatctcg
     3841 ccgggaaata atgcctagat cgagttggtt gtgcacacga ccatgtcggc gtctttgatc
     3901 gcttcggcgg cgctagccga ctcgatcaat tcggcattaa cctggccgcg ccattcatcg
     3961 acaaacctgc ggcgcgattc cggcgtcggg ctgaacactc ggacctgttt cagatctcga
     4021 accatcgcca tcgtgaccag atgcgcactc gcctgccagc cagaaccgag taaagccatc
     4081 ctggtggaat tcttgcgcgc cagatagcgc gctgcgatcg cgctggcgcc ggcgacacgc
     4141 aacttctgca catagccgtc gggaaaaatc gccagcggct cgccggtctc ggtgctgaac
     4201 agcatgatta gcccgacgta gcggtcgccg ccggccaccg gcagtttgtc cttgcgtata
     4261 ccgcccgcct tctcgtacca cttgaccacg ctggagttga gccgcagcgc ggcgatgccg
     4321 cgattgggca gcacgccgct catagtcttg tactcgtaga actcatttga tgacttcgtc
     4381 gggctgaaga cgtcatagcg cggcaccgtc gccgccatgc cgttgccgag atcgcgatag
     4441 gcgtcttcga gcacgtcgag gcaagcgccc accggcagaa ttttttcgat ctcatcgttg
     4501 gaaagaatca gcatgaccaa cctcctgagc ccagcgcgca gccattgtta gtgctgtccg
     4561 gtgcgctttt caaggagcct ctatgcccat caagcggttg acagcccagc agcgctgttg
     4621 tcttatcaga cttccaaaac tcgcaacttc agccaaggta atcatgtcat tacacctgaa
     4681 cgcaaaaacc gtaagacgtt tttcatttat cggcttcctc gtcgcggcta ttttcgccgc
     4741 gcccttcgcc cgcgccaacc cctatctagc aaagccgggc gaagcgccgg tcaccgtgta
     4801 tgtggccacc tgcgctgtta ccggcggctt tatccaactc tacgccgccc tcgaccagaa
     4861 cctgtttgaa aaatacggca tcgcgatcaa gcatgtcgtc atccgcggcg gcacgaatat
     4921 caatctcgcc gccctcggcg ccgacgaagt gcagttcctc tattgcgccg ccgactcgac
     4981 cctgccgggt atggccgtgg gccgcgacgc catcctggtc gcttcgccct tggtcggttt
     5041 gccctacgta atcatcgcgc gcaaggaaat ccgcacggtg caagatttga aaggaaagtc
     5101 catcggcgtc ggcagcgtcg ccggcctgcc ctaccgcctg ctaagaattt tcgtgaagaa
     5161 gttcgggctt aacgacacgc agatccgccc cgtaggcggt agccagccgg agcgctacaa
     5221 cgccatgatg cagggcgtta tcgattccgg gccattcaca ccgccaatgg acgcgcgcgg
     5281 caaaaaagat ggattcaatc tgatctacca tatgaatgac ttggggctgc cggcgatata
     5341 cagctcgctg cacaccaacg ccaaatcctt gcgagagcgc cgacagaccg tccagcgctt
     5401 cgtcgcggcg ctggcggaag ccgtcgcttt tgttgaaaac aatcccgaaa aggccaaggc
     5461 gtcggtggcg aaaatcctca gagtcaacga cgaagaggcg ttgcaatcct cttacgatgc
     5521 ctatgccaag aagcatgtga accggcgcct gatcgtgccg ctcgacgcag tggcggattc
     5581 cgtggaagtg gcgcgcgacg ccggcgccaa agtcagcaag aaagccaatg agatcgtcga
     5641 caacagtttc gctgaaaatc tgcagaagag cggatttttg aaggagctat ggggcggaaa
     5701 gctgcccaat taatttttgg agaagaccca tgctacaacc atccggtctg gtaagcggcc
     5761 attatgaatg tcgatcgttc gccgaatcct tgccgatcct caaggagttt ctcgccctga
     5821 gcgtggttga tgaaaaagcc gatgagaaaa ttcttcagca tccaaacacc ggctggcagc
     5881 tcgtagtcca cgccaacggc tccgccgcgc cgatcaaatc catgcgcaac cactatggtg
     5941 tgcgtgtcgc caccaacgcc gaagtggata ccgcgacgga atatttggag agccgcaaga
     6001 accagttcgg catcaaagtg atcaagccgc gtgagaacca caacgcctac tcggtccatt
     6061 tctacgagcc cggcggcaac tattgggaaa tcgaatcgta tgaagatcgc cacaaagcag
     6121 gcctgccgtt tgacgtctcg tttccgtgga aaacgcctct caccgaggag cagtttcccg
     6181 gcagaggcta cgtgcctcag gcattcacac atggcaccat tgaatgcaac gactgggcct
     6241 catccgtaaa tttctacaaa caaggtttgg ggatggatgt tatcacccac atcgacacgc
     6301 ccaagccgca caacattaag catcctgcca agccgtggta cgtagtaagc ctggaagttc
     6361 ctgaaaaaaa tcgaaaatac ttaggcctca tgcagcgct
//
LOCUS       DASQKS010000126         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2167891, whole genome shotgun sequence.
ACCESSION   DASQKS010000126 DASQKS010000000
VERSION     DASQKS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167891"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..598
                     /locus_tag="VE170_03275"
     CDS             134..598
                     /locus_tag="VE170_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ14482.1"
                     /translation="MKKTISLRINGRERSAEVEARTTLLDLVREQFGLTGAKLGCDIQ
                     VCGACTLLVDGKPVSACSMLAVDADGCAIESVEGLSSKDKLHPIQEAFMEFGALQCGY
                     CTSGFILTAKALLAECPQPSDQQIRDYLAGNFCRCGCYQEIMQAVKNVRGEV"
     gene            643..2964
                     /locus_tag="VE170_03280"
     CDS             643..2964
                     /locus_tag="VE170_03280"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ14483.1"
                     /translation="MASTEIGKSYRRLDYETKVTGRAQYLADMSVPGMLHGKILRSPY
                     PHARITRIDASKAEKVAGVMAVLTRGDILHDQGIEPFYGPVFKDQTIVATEKVRHVGD
                     PVAAVAALTVDAAEAALKLIEVDYEELPAVLNIDDALKPGAMLVHDSVKLPASGFADL
                     ADLKPIDGTNLCTHFKLNRGDIDAGFAAADHVFEDTFTLPATQHCFLETHACIASVEP
                     GGRITVWATTQNPFVVRTQLANIFKVPVAKVRVIVPYLGGGYGGKVYPKVEPITVALS
                     QKAGRPVRLVLSREEVFYTITKHAAVIRMKTGVKKDGTLIARECEIHLDTGAYAEIGP
                     RVAKKSGYTAAGPYKIANLKIDSYSVYTNKPPAGAFRGFGVSQSAWAVESQMDIIAAA
                     LKIDPVELRKRNGYDDGDKFVTEETLRAVGLKECIDEVARSIGWEKKTFKGSSVQGST
                     GGSLRRGKGLACMIKATITPSISCAVVKLNEDASLSIYSGTVEMGQGSETVLAQIAGT
                     ELGVPMPTIQVLGVDTDVVPYDLTTSSSRSTFHMGKAVQLAAQDIKRQLKQIVVKEYG
                     VPEDKIDFAGGKIRLPETQLDYAEVMFKRFGMQGGTLVGEGQVKTATKDEFGEKSTSA
                     FWFLAAGAAEVEVDIDTGKFRLIKYASAVDVGKALNPLGCQQQLAGAAITGMGQAMFE
                     EIAYDNGQLINPNLVDYILPSLGDMPLVVESIAVEMPDRNGPFGAKGIGESALIPVAP
                     AIANAIYDAVGVRIKDLPIKAEKIFLGLAELDA"
     gene            3005..3463
                     /locus_tag="VE170_03285"
     CDS             3005..3463
                     /locus_tag="VE170_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ14484.1"
                     /translation="MRFNAKHQSRFAGALALVLTLGGLSSCLTGYLEEDLKRQREQIE
                     QQNLELAHQRKELEAIAGGQKQQQDCSRAFRDYFDKAQLSANRDERISLYRQGLQLCP
                     DDDVAHYELGRDLVDAGQRGEAEQEFEAALKINPGFAEARRRLDNLRGSR"
     gene            3541..4311
                     /locus_tag="VE170_03290"
     CDS             3541..4311
                     /locus_tag="VE170_03290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYJ14485.1"
                     /translation="MNTLTALTLALIMASTVYALDDKKLVDLTYEFSAETHHWPTAKP
                     FHLEKVAEGRTPGGFWYSSYNYGGSEHVGTHLDAPFHFAEGKRTAEQIPLSQLIGPGV
                     IIDVSRLAGKDADYTLQLRDVGAWEKAHGRIPSGAIVLIRSGWGKFWGDRKRYFGSDT
                     PGDADNLHFPGLSKEVAEFLVKQRRIKAVGIDTPSIDHGPSKEFLAHQILGAANLPIF
                     ENVANLDRLPAKNTTIYAIPMKIKAGSGAPLRIFAILP"
     gene            4432..>4685
                     /locus_tag="VE170_03295"
     CDS             4432..>4685
                     /locus_tag="VE170_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14486.1"
                     /translation="MIGVSNFTTWLLACALTQVTALAAVAHAAEIQMQIQAAPGAQAP
                     LSPTGKLLTIEDAVRIGLENHPRIRQANERILSQQAVVGQ"
BASE COUNT         1051 a         1276 c         1434 g          924 t
ORIGIN      
        1 tgcggcgcag gagtgcagtc cgaccagcga cctgcgcggc tcggagaaat ataagcgtgc
       61 gattgtgggc acgctggtga agcgcgcggc gatgaaagcg aacgaacgtg ctgcacaaat
      121 gtgattcgaa atcgtgaaaa aaaccatttc acttagaatt aacggcaggg agcgcagcgc
      181 tgaagtcgag gcgcgcacga cgctgttgga cttggtgcgg gagcagtttg ggcttactgg
      241 cgccaagttg ggctgcgaca tacaggtttg cggcgcgtgc acgttgttgg tcgatggcaa
      301 gccggtcagc gcctgttcga tgttagctgt cgacgcggac ggttgcgcaa ttgagagcgt
      361 cgaaggcttg agtagcaagg acaagctgca tccaattcag gaagcgttta tggaatttgg
      421 cgcgctgcag tgcggctact gtacgtccgg cttcattctc acggccaaag cgctgctcgc
      481 cgaatgtccc cagccatcgg atcagcagat tcgcgattat ctcgccggca acttttgtcg
      541 ctgcggctgt tatcaggaga tcatgcaagc ggtgaagaac gttaggggtg aggtgtaagg
      601 acggcattca gcacacgagt attaaggttg tagagagcaa aaatggcatc cactgaaatc
      661 ggcaaatcct atcgccggct cgattacgaa acaaaagtga ccggacgggc gcagtatctg
      721 gcggacatga gcgtgccggg aatgctgcac gggaaaattc tccgcagccc gtatccgcat
      781 gcgcggatta cgcgcatcga cgcgagcaag gctgagaagg ttgccggtgt catggcggtg
      841 ctgacgcgcg gtgatatcct gcacgaccag ggcatagagc cgttttacgg gccggtgttc
      901 aaggaccaga ccattgttgc gacggagaag gtgcgccatg tcggcgatcc ggtggcggcg
      961 gtggcggcat tgaccgtcga tgccgccgaa gcggcgctca aactgatcga ggtcgattat
     1021 gaagagctgc cggcggtgct caacattgac gatgcgctca agcccggtgc gatgctggtt
     1081 cacgactcgg tcaagctgcc ggcgtccggt tttgcagatc tcgccgactt gaagcccatc
     1141 gacggcacta acctgtgcac ccatttcaag ctcaaccgcg gcgacatcga tgcagggttc
     1201 gccgcagcgg atcatgtttt cgaagatacc ttcaccttac cggcaacgca gcattgtttt
     1261 ctcgagaccc acgcatgcat cgcgtcggtc gagcccggcg gacgcatcac ggtctgggcg
     1321 acgacgcaaa atccttttgt cgtacgcacc cagctggcga atatattcaa agttccggtg
     1381 gcgaaggtgc gcgtgatcgt gccttacctc ggcggcggct acggcggcaa agtttatccc
     1441 aaggtcgaac cgattaccgt ggctctgtcg cagaaagccg gccggccggt gcgcttggtg
     1501 ctttcccgtg aagaagtttt ttacactatt accaagcatg cggcggtaat ccgcatgaag
     1561 accggcgtaa aaaaagacgg cacgctgatc gcgcgcgagt gcgagatcca tctcgacacc
     1621 ggcgcctacg ccgagatcgg cccgcgggtg gcgaaaaaat ccggctacac ggcggcggga
     1681 ccgtataaga tcgccaactt aaaaattgat tcctactcgg tctataccaa taagccgccg
     1741 gccggcgcgt tccgcggctt cggcgtatcg cagtcggcct gggcggtgga gtcgcagatg
     1801 gacatcatcg ccgccgcgct caagatcgac ccggtcgagc tgcgcaagcg caacggctac
     1861 gacgacggcg acaagtttgt caccgaagaa acgttgcgcg cggtgggatt gaaagagtgt
     1921 attgatgaag tggcgagatc tatcggatgg gagaagaaaa cgttcaaggg ttcaagtgtt
     1981 caaggttcaa ccggagggag cttgcgtcgc ggcaaagggt tggcctgcat gatcaaggcg
     2041 acgattacgc cgtcgatttc ctgcgcggtg gtgaagctca acgaggatgc cagcctatct
     2101 atatatagcg gcaccgtgga gatggggcag ggctcggaga ccgtgctggc gcagatcgcc
     2161 ggcacggagt tgggtgttcc gatgccgacc attcaagtgc tcggcgtcga taccgacgtg
     2221 gtgccgtacg atttgaccac atcgtcgagc cgttcgacct tccacatggg caaggctgtg
     2281 cagctcgccg cccaggatat caagcgccaa ctcaagcaga tcgtggtcaa ggaatacggc
     2341 gtgccggaag acaaaatcga tttcgccggc ggcaagattc gtttgccgga aacgcagctc
     2401 gactatgccg aggtgatgtt caagcgcttt ggcatgcagg gcggcacctt ggtcggcgaa
     2461 ggacaggtga agactgcgac caaagacgag ttcggcgaaa agagcacatc ggctttttgg
     2521 tttctcgccg ccggcgccgc cgaagtggaa gtcgacatcg acaccgggaa attcagactg
     2581 atcaaatacg ccagcgccgt cgacgttggc aaggcgctca atccgctcgg ctgccaacag
     2641 cagttggccg gcgcggcgat caccggcatg ggccaggcaa tgttcgagga gatcgcctac
     2701 gataacggcc aattgatcaa tccgaactta gtcgattata tcctgccttc ccttggcgac
     2761 atgcctttgg tagtagagtc gatcgcggtc gagatgccgg accgcaacgg gccctttggc
     2821 gccaagggca tcggcgaaag cgcgttgatt ccggtcgccc ccgccatcgc caacgcgatc
     2881 tacgacgccg tcggtgtgcg catcaaagat ctgccgatta aagcggaaaa gatttttctt
     2941 gggttggcgg agctcgatgc gtaagcaact gtttcggtaa agtcttggac aaagcggaga
     3001 gaagatgcga ttcaatgcca aacaccaatc caggtttgcc ggtgcgctcg cgctggtgtt
     3061 gaccctgggg ggattgtctt cctgcttgac tggatatctc gaggaagatc tcaaacggca
     3121 gcgggaacaa atcgaacaac aaaatcttga gttggcgcac caacgcaaag agctggaggc
     3181 gatcgccggc ggccagaagc aacagcagga ctgcagccgg gctttccgcg attacttcga
     3241 caaagcacag ttgtcggcca accgcgacga gcggatttcg ctgtaccgcc aaggcttgca
     3301 gctttgtccc gacgatgatg tggcacatta tgagcttggc cgcgacctag tcgatgccgg
     3361 ccagcgcggc gaagcggaac aggaattcga agcggcgctc aagatcaatc ctggttttgc
     3421 cgaagcgcgc cgccgcctcg ataacctgcg tggaagccgc taattcctcg gccaacctgc
     3481 accgggtcat gcccaagcag atcctcgcgc cagactcttc aaagttcgga ggtttcgttt
     3541 atgaatacgc taaccgccct gacgttggcg ctgataatgg caagtacggt atatgcgctc
     3601 gatgacaaaa aactcgtcga tctgacctac gagttcagcg ctgaaaccca ccattggccg
     3661 acggcgaaac cttttcacct cgaaaaagtc gcggaaggta gaaccccggg cggcttctgg
     3721 tattcgtcct ataactacgg cggctcggag catgtcggta cgcacttgga cgcgccgttt
     3781 catttcgccg agggcaaaag gaccgccgag caaattccac tctcgcaact gattggaccc
     3841 ggagtgatca tcgacgtcag ccgcctggct gggaaagacg ccgactacac tttgcagttg
     3901 cgcgacgtcg gcgcttggga gaaagcccac gggcgaattc ccagcggagc gatcgtattg
     3961 attcgttcgg gctggggcaa gttctggggc gaccgcaaac gttacttcgg tagcgacacg
     4021 ccgggcgacg ccgacaatct ccatttcccc ggcctgtcta aagaggtagc cgaatttctc
     4081 gtcaagcagc ggcggatcaa agccgtcggc atcgacaccc cgagtatcga tcacggaccg
     4141 tctaaagaat ttctagccca tcaaatactt ggcgcggcaa atctgccgat ttttgaaaac
     4201 gtcgctaacc tggatcgctt gccagcgaaa aatacgacga tttacgcgat tccgatgaaa
     4261 atcaaagccg gcagcggcgc gccgctgcga atcttcgcta tcctgcccta agttcgaagc
     4321 ggcggttgag gctgcgccgg accgcgtttt cggttctagc actttagcgc gattttgcat
     4381 aagaacggta aagccggtag aatttagcgc gcttatgtaa gggggaaaag tatgatcggg
     4441 gttagcaatt ttacaacatg gctgcttgcc tgtgccttga cacaagtgac ggcgctcgcc
     4501 gccgttgccc atgcggccga gattcagatg cagattcagg cggcaccggg agctcaagcg
     4561 ccgctatcac cgaccggaaa actgctgacg atcgaagacg cggtgcgcat cggtttggag
     4621 aatcatccgc gcatcagaca ggctaacgaa cggattttgt ctcagcaggc ggttgtcggc
     4681 cagca
//
LOCUS       DASQKS010000127         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5962649, whole genome shotgun sequence.
ACCESSION   DASQKS010000127 DASQKS010000000
VERSION     DASQKS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5962649"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VE170_03300"
     CDS             <1..683
                     /locus_tag="VE170_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14487.1"
                     /translation="DLGMYPDGVAPTLHSDAVSPAATPAAVEAERSWTRAEAVQTSAL
                     WLLVASVFLASLGTGGIAFHTVAYFTDVKISPAVAAGAVSVMALSGAFGNGIWGALAE
                     RLSPRSLSVTATALSAASVVVLMSVDSPPSAYLFGVLFGICARGGTAVLTQIMLAHYF
                     GRRSFGAISGVLEPFHKGGLGAGALVAGLAFDWSGNYRLALLVFLTSYLVSALLVFFA
                     RRPPLPSA"
     gene            759..2084
                     /locus_tag="VE170_03305"
     CDS             759..2084
                     /locus_tag="VE170_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYJ14488.1"
                     /translation="MVSSRPLGFLIVLFLLFPFAPAHAAQVQEFVLDNGLKVLLLEDH
                     KSPAVTFQVWYRVGGRDEKDGKSGLAHFLEHMLFKGTPTTQPEEYSRIIAKNGGRSNA
                     FTTSDMTVYFATMSRDKIHIQLELEADRMANALLGDTYFEPEKNVIQEERRLRTDDNP
                     GSALYEIAASVAYTIHPYRRPVVGWMQDIQSLTRQDLVDFYKLYYAPNNAVIVMVGDF
                     DSNEMLAKIKAAYGKIPRGSEPPKLTLVEPSQRGEKRVTLKKEAELPMLMLFHHAPNL
                     HSPDSFALDLLSIVLAGGKSSRLYQELVYQKRLVRGIDADYSAVSKDATGFSISAQLL
                     PGAEPANVEQQIDRSLDKVKTQLISERELQKAKNQVEAAFIFAQDSIFGQAMKIGYYE
                     MVGGWRLVDSYLDNMRKVTREDIRRVAQKYLQRDNRTVGTLIPIKAKTQ"
     gene            2081..>2959
                     /locus_tag="VE170_03310"
     CDS             2081..>2959
                     /locus_tag="VE170_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYJ14489.1"
                     /translation="MMRTLLGLFLFGLLAQALPAQGAMVPKRSVLSNGLVLLTSEQRA
                     LPTVAIELLIDAGARHESAEHTGLAHLTSQLLTDGTAKRSALQISEALDFIGASLDTG
                     CADDLATISLTILKKDLSVGLQLLAEILTGASFPPAEIERQKQAIIASIKAKEEDPGT
                     LAGDAFAAALLPGSAYGRPVEGTEASVKRLDRQRVQEFFKRYYRPNRTIISVVGDVSE
                     REISVALTEAFKNWPKGEATRAPTAPVQSDVAKILRVNKDLTQANIILGHGGVPRGHP
                     DYYAIQVMNYILGGGGF"
BASE COUNT          591 a          823 c          894 g          651 t
ORIGIN      
        1 aagatttggg catgtatccc gacggggtcg caccgactct acactccgac gctgtctcgc
       61 cggccgcgac gccggctgct gtcgaagccg agcgcagttg gacgcgcgcc gaggcggtgc
      121 agacgagtgc gctatggttg ctcgtggcga gcgtctttct cgcctccctc ggcactggcg
      181 gtatcgcttt tcacacggtc gcttatttta ccgatgtgaa gatttcaccg gcggtcgccg
      241 ccggcgcggt gagcgtgatg gcgctctccg gggcatttgg caatggcatc tggggagcct
      301 tggccgagcg gctttcgccg cgcagtttga gcgtgacggc gacggcgttg tcggcggcgt
      361 cggtggtggt gttgatgtct gtggattcgc cgccatcggc atatctcttc ggcgtgctct
      421 tcggcatctg cgcccgcggc ggcacagcgg tgttgacaca aatcatgctg gcgcactatt
      481 tcggccggcg ctccttcggc gccatcagcg gcgtgctcga accgtttcac aagggcggcc
      541 tcggcgccgg cgccttagtc gccggcctgg ccttcgattg gagcggtaat taccgcttag
      601 ctttgctggt ttttctcacc agctatctgg tgtccgcgct gttggtgttc tttgcgcgcc
      661 ggccgccctt gccatctgcg tagattcggc atccgcgggg cgaatgttgc ggcgctggcg
      721 atcttgactt tgaggcgtca tcctttagct tgatcaccat ggtttcgtcg cgtcctctcg
      781 gtttcttgat cgttttgttt ttgctgttcc cattcgcgcc cgcccatgcc gcgcaagtcc
      841 aagaattcgt cctcgacaat ggtctgaaag tgttgctctt ggaagatcat aaaagtccgg
      901 cggtgacgtt ccaggtgtgg taccgagtcg gtggccgtga tgagaaagac ggcaagagcg
      961 gcctggccca tttcctcgaa catatgttgt tcaagggcac gccgacgacg cagccggaag
     1021 agtactcgcg gattatcgcc aagaacggcg gccggtccaa cgcttttacg acatcggaca
     1081 tgacagtcta cttcgcgacc atgagccggg acaagattca tattcagctc gagctggaag
     1141 ctgaccggat ggccaatgcg ctgttgggcg acacttattt cgagccggaa aagaacgtga
     1201 tccaagaaga gcgccgcttg cgcaccgacg acaatcccgg ttcggcgctt tatgagatcg
     1261 ccgcatcagt ggcctacact attcatccat accgccgtcc cgtggtcggc tggatgcagg
     1321 atattcaaag cctgacgcgc caggacttgg tggattttta caaactttac tatgcgccca
     1381 acaacgcggt gatcgtcatg gttggcgatt tcgattcgaa cgaaatgctg gccaaaatca
     1441 aagccgccta cggcaagatt ccgcgcggct ccgaaccgcc aaagctgacg ctggtcgagc
     1501 cgtcccagcg cggtgaaaag cgcgtgacgc tgaaaaaaga agcagaactg ccgatgctga
     1561 tgctgtttca tcacgcgccc aatctgcaca gccccgacag ttttgcgctg gatctgctat
     1621 caatcgtgtt ggcgggcggc aagagttcgc gtttgtatca agagctggtg taccaaaagc
     1681 gtttggtgcg cggcatcgat gccgactata gtgcggtgtc gaaggacgcc acagggtttt
     1741 caatatccgc gcaactgctg cccggcgctg aaccggccaa tgtcgagcag caaattgacc
     1801 gctcgctcga caaggtcaaa acccaattga ttagcgagcg cgaattgcaa aaagccaaaa
     1861 atcaagttga agcggctttt atcttcgccc aagactcgat cttcggccag gcgatgaaga
     1921 tcggttacta cgaaatggtc ggtggctggc gcttggtcga tagctatctc gacaatatgc
     1981 gtaaagtcac tcgggaagat attcgccgtg tggcgcagaa atatctccaa cgggacaatc
     2041 gtacggtcgg caccttgatt ccgattaaag ctaaaactca atgatgcgta ctttactcgg
     2101 gcttttctta ttcggtttgt tggcgcaggc tctgccggca cagggcgcca tggtgccgaa
     2161 gcgaagcgtg ctcagcaatg gattggtgtt gctgacctcc gagcagcggg ctttaccgac
     2221 ggttgccatc gagttgttga tcgatgccgg cgcgcgtcat gagagcgcgg aacataccgg
     2281 cctggcgcat ctaacctcgc aacttttgac cgacggtacg gccaagcgaa gcgcactgca
     2341 aatcagcgaa gcgttggatt tcatcggcgc tagtctcgat accggctgcg ctgacgactt
     2401 ggcgacgatt agcttgacga tactcaagaa ggacctgagc gttggcttgc agttgctcgc
     2461 cgagatcctg accggcgcga gttttccgcc ggcggagatc gagcggcaaa agcaagcgat
     2521 catcgcctcg atcaaggcga aagaagaaga tcccggcacc ttggcgggcg atgcgtttgc
     2581 cgcagcgtta cttcccggca gcgcttatgg ccggccggtg gagggaacgg aagcttcggt
     2641 aaaacggttg gatcggcaac gcgtgcaaga attttttaag cgctactatc gtcccaatcg
     2701 gacgatcata tcggtggtcg gcgatgtttc tgagcgggaa attagcgtgg cgctcaccga
     2761 agcattcaag aactggccca aaggcgaagc gaccagagcg ccaacggctc cggtgcagag
     2821 cgatgtcgcg aaaatcctgc gggtcaacaa ggatctgacg caagccaata tcatcctcgg
     2881 tcatggcggg gtgccgcggg gccatcccga ctactacgca atccaggtga tgaactacat
     2941 tctcggcggc ggcgggttt
//
LOCUS       DASQKS010000128         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_493494, whole genome shotgun sequence.
ACCESSION   DASQKS010000128 DASQKS010000000
VERSION     DASQKS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493494"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..792
                     /locus_tag="VE170_03315"
     CDS             172..792
                     /locus_tag="VE170_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14490.1"
                     /translation="MLLCFIPPAWPSRHYTAKQMEALATRVGQTFWSQPVDGKGPSFA
                     TAPAANAKLVRIDTVESFRIEALVGQAAKNPYYKIKRENGQDGYISPEQFLEGFNLTI
                     VTTDPAAEEKRKSAEAAEADKKRIEWIRAQPWSPAVKEAAIKRQPVPGLTNSEIKQVL
                     GAPSRITKVQGPIKVAEEHWLYPDGSLLIFHNGLLSKIERPDNNKK"
     gene            complement(807..1307)
                     /locus_tag="VE170_03320"
     CDS             complement(807..1307)
                     /locus_tag="VE170_03320"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYJ14491.1"
                     /translation="MDLIIDGYNLLALDKRLDRGLEQSRNSLINKLARYRQAKPMNLT
                     VVFDGWRSGSASETRLAQDGIAIVYSRLGEKADAVVIRLAREKNNGAVVVSSDREIRS
                     AVERFGAVAIHAQEFSEILHELDDGGGADGFFNDWQDRPSDRGGANRLSKAERKRQDK
                     LKKLRL"
     gene            1428..2600
                     /locus_tag="VE170_03325"
     CDS             1428..2600
                     /locus_tag="VE170_03325"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HYJ14492.1"
                     /translation="MSLTIKKAKRINDLPPYLFAEIDRRKREALSRGVDLIDLGIGDP
                     DIPTPATVVEKLLEAASRPANHRYPNSSGMAEFRQAVSTWYKSRFGVALDPGKEVCSL
                     IGSKEGIGNMAVAFVDPGDIVLVSSPCYPVYHIGTAFNGGKNYFLPLKKENHFLPDLD
                     AIPADVAKQAKLLWINYPNNPTAAVADKDFYQRVVAFANKYNVIVCHDAAYTEMGYDG
                     YRPMSFLEVDGAREVGIEFHSLSKTFNMTGWRIGMAVGNPEIVGGLAQAKSNLDSGIF
                     QAVQEAGIEALRLGDSIVEPSRKIYQERRDILVDGLRAVGLECDKPKATFYVWVSCPA
                     GLSSTDFTSKLLDEAGVVTTPGNGFGDAGEGYVRFTVCVDKERLREVAERIRRVKL"
     gene            2624..3127
                     /gene="folK"
                     /locus_tag="VE170_03330"
     CDS             2624..3127
                     /gene="folK"
                     /locus_tag="VE170_03330"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYJ14493.1"
                     /translation="MASVITHQVYIGIGSNVGNKRENFFEAVERVGKLPDTRVIKESS
                     LYESEPLGDAKDWYVNGAIEIETKFKPEALLEKFKNIERVMGRKKVKKRWGARIIDLD
                     ILLFDLAVVKKKNLRVPHPEMASRKFVLIPLSEIAPQVIHPELGVTISELLINVKDDK
                     KIHLYHV"
     gene            3160..3387
                     /locus_tag="VE170_03335"
     CDS             3160..3387
                     /locus_tag="VE170_03335"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HYJ14494.1"
                     /translation="MTLLRLLFLGLVIYIVYAALKPLLRGGKKSPDPARLGEQMVLDP
                     QCQTYVPQSAAVARHGKFFCSEECAQRYLTG"
     gene            complement(3376..>4302)
                     /locus_tag="VE170_03340"
     CDS             complement(3376..>4302)
                     /locus_tag="VE170_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYJ14495.1"
                     /translation="VLEGDEYDTAFFDKGPKFLHYRPRHVILTSVEFDHADIYRDLDH
                     VKSAFAKLVEIIPADGSLLVCSDYPDAVALSRAAHAPTATYGESGDWTAQNVRFDHGR
                     SFFEPCFKGRSEGLIEAAVIGLHNVKNALAVYAMGRIMGIDGEKLRGGFSTFKGVKRR
                     QEVRGERRGVTVIDDFAHHPTAVRETIDAVRGAYAGRRLWAIFEPRSNTSKRNIFTKE
                     FAAALALADVTIVAGVYQPERVPENQRLSVPDLLGEINRIGGAGRALTIADSAAIADY
                     VARHSRAGDVLLVMSNGGFDGVHEKILQALAG"
BASE COUNT          981 a         1268 c         1198 g          855 t
ORIGIN      
        1 gtttcgcagt tggcggagca gtttccgctt gcgtcgccgt ccagagctgg taaattttat
       61 aaagcaaaga ttttccacgt gtagagtcaa ggtttccgac gaaacgatcg atcgatgaag
      121 cacccaacta agaaaaatcg cgtcagactt gccggatggc tatgcgcttt tatgctgctc
      181 tgtttcattc ctccggcttg gccctcgcgc cactacaccg ccaaacagat ggaagccctg
      241 gccacccggg tggggcaaac cttttggagc caaccggtgg acggcaaagg gccgagcttt
      301 gcgaccgcgc cggcagcgaa cgccaaattg gtgcggatcg acacggtgga atcctttcgg
      361 attgaggcgc tggtgggcca agcagccaag aacccgtact acaagatcaa gcgtgagaac
      421 ggccaagacg gttacatcag ccctgagcaa tttctcgaag ggttcaatct gacgattgtc
      481 acgaccgatc ccgctgctga agaaaagcga aaaagcgccg aagccgccga ggccgacaaa
      541 aaacggatcg aatggatccg cgcccaacca tggtcgccgg cggtcaagga ggccgcgata
      601 aaacgacagc cggtgccggg attgacgaac tccgaaatca aacaggtgtt gggggcgccc
      661 agccggataa ccaaagtgca gggtccgatc aaggtcgccg aggagcattg gctttaccct
      721 gacggctcgt tgctgatatt ccacaacggt ttattgagca agatcgagcg gccggacaac
      781 aataaaaaat aacctgcgcc ggcaatctag aggcggagct ttttgagttt atcctggcgc
      841 ttgcgctccg ccttcgacaa tcggttggcg ccgccgcgat cggatgggcg atcttgccaa
      901 tcattaaaaa acccatccgc gccaccgcca tcgtccaact cgtgtaagat ctcgctaaat
      961 tcctgggcgt gaatcgccac cgcgccgaag cgttccacgg cgctacgaat ttcacgatcc
     1021 gacgacacca ccaccgcgcc attatttttt tcccgcgcca gacggatgac cacggcgtcg
     1081 gccttttcgc ccaagcgcga atagacgatg gcgatgccgt cttgtgcgag acgggtttcg
     1141 ctggcggaac cggaacgcca gccatcgaac acgactgtca agttcattgg cttggcttgg
     1201 cggtagcgcg ctaatttatt gatcagcgaa ttacggctct gttccagccc gcgatccaag
     1261 cgcttgtcga gcgcgagcaa gttgtagcca tcgataatca ggtccatagt caacgcatcc
     1321 taaccacttc acccgacgat ggcaaaaacc ggaattaaac ggatggttgt caaatctgca
     1381 aaaaacaggt aaaaacaggt cgattttctc acggaggacc gcgagcattg tccctcacca
     1441 taaaaaaagc gaaacgaatc aacgatttac caccgtacct ctttgccgaa atcgaccgcc
     1501 gcaagcgtga agcgctgagc cgcggcgtcg acctcatcga tcttggcatc ggcgatcccg
     1561 atatcccgac gccggcgacc gtcgtcgaga aattgcttga agccgccagc cggccggcca
     1621 accatcgcta tcccaatagc tcgggaatgg cggaattccg ccaggcggta tcgacctggt
     1681 ataaatcccg tttcggcgtc gctctcgacc cgggcaaaga ggtttgttcg ttgatcggct
     1741 ccaaggaagg catcggcaat atggccgtgg ccttcgttga tcctggcgat atcgttctgg
     1801 tttcgagtcc ctgctatccc gtctaccata tcggcaccgc gttcaatggc ggcaagaatt
     1861 atttcctgcc gttgaagaaa gaaaatcatt ttctgccgga cctggacgca atcccagcgg
     1921 acgtggccaa gcaagccaaa ttgctctgga tcaattatcc gaacaacccc accgcggcgg
     1981 tggcggacaa agatttttac cagcgggtgg tcgccttcgc caacaagtat aatgtcatcg
     2041 tctgccatga tgctgcctat accgaaatgg gttacgacgg ttatcggccg atgagctttc
     2101 tcgaggtcga cggcgcccgc gaagtcggca tcgaatttca ttcgctatcg aaaaccttca
     2161 acatgaccgg ctggcgcatc ggcatggcgg tgggcaatcc ggaaatcgtc ggcggcttgg
     2221 cgcaagcgaa atccaatctc gactccggca tcttccaggc ggtgcaggaa gccggcatcg
     2281 aagcgctccg gctgggcgac tccatcgtcg agccatcgcg caagatttac caagagcgcc
     2341 gggatatttt agtcgacggc ctcagagccg tcggtctcga gtgcgacaag cccaaggcga
     2401 cattctacgt ctgggtgagc tgtcccgcag gactctcctc caccgacttc accagcaaac
     2461 tgctcgacga agccggcgtg gtcacgactc ccggcaatgg tttcggtgac gccggcgaag
     2521 gctatgtccg tttcacggta tgcgtcgata aagaacgact ccgggaggta gcggaaagga
     2581 ttcgccgcgt caagctctag cacggccttt cccggacggc accatggcga gcgtcatcac
     2641 ccatcaagtc tacatcggca tcggctccaa tgtcggcaac aaacgggaaa atttttttga
     2701 agccgtagag cgcgtcggca aactgcccga cactcgggtg atcaaggaat cctcgcttta
     2761 tgaaagcgag ccgctgggcg acgccaaaga ctggtacgtt aacggcgcca tagaaatcga
     2821 aaccaagttc aaaccggaag cgctgctcga gaagttcaaa aacatcgagc gcgtcatggg
     2881 ccgcaagaag gtgaaaaagc gctggggcgc gcgaatcatc gatctcgata tcttgctatt
     2941 cgacctggcg gtggtgaaaa aaaagaacct gcgcgtgccg cacccggaga tggccagccg
     3001 caagtttgtg ctgattccct tgagcgagat cgcgccgcag gtgatccatc ccgagctcgg
     3061 cgtaaccatt tccgaacttt tgatcaacgt caaagacgac aagaagattc atctctatca
     3121 tgtctgaggc ctttcccctg ccgccgagct aggagcgaca tgaccctgct gcgactgctg
     3181 tttcttggcc tcgtcatcta catcgtctat gcggcactca agccgctgct gcgcggcggc
     3241 aagaaatcgc cggatccagc acgactcggc gaacagatgg tcctcgaccc gcaatgccaa
     3301 acctatgtgc cgcaatcggc cgcggtggcg cgccacggaa agtttttttg cagcgaagaa
     3361 tgcgctcagc gctatttaac cggctagagc ctgcagaatc ttctcatgca cgccgtcgaa
     3421 accgccgttg gacatcacta gcagcacatc gcctgcccgg ctatggcgcg cgacatagtc
     3481 agcgatggcc gccgagtcgg caatcgtcag cgcccgcccg gcgccgccaa tgcgattaat
     3541 ttcgccgagc aagtctggca ccgataagcg ttgattttcc ggcactcgct cgggctgata
     3601 aacgccggca acgatggtaa cgtcggccag cgccaacgcc gcggcgaatt cttttgtgaa
     3661 aatattgcgt ttgctggtgt tgctgcgcgg ctcgaagatc gcccagagcc ggcgaccggc
     3721 gtaagcgccg cgcaccgcat cgatggtttc gcgcaccgcg gtgggatgat gggcgaagtc
     3781 gtcgatgacg gttacgccgc gcctttcgcc gcgcacttct tggcgccgct tgaccccctt
     3841 aaaagtgcta aagccgccgc gcagtttttc gccgtcgatt cccatgattc ggcccatggc
     3901 gtaaaccgcg agagcgtttt tgacgttatg aagaccgatg accgccgcct cgatcaaacc
     3961 ttcggatcgt cctttgaaac agggttcgaa gaagcttcga ccgtggtcga acctgacgtt
     4021 ttgcgcggtc caatcaccgg actcgccata agtcgccgtc ggtgcatgcg ccgcgcggct
     4081 cagcgccacc gcgtcaggat agtcgctgca aaccagcagc gacccatctg cgggaataat
     4141 ttcgacaagc ttggcaaagg cactcttgac atgatcgagg tcgcggtaaa tatcggcgtg
     4201 gtcgaactcg acgctggtca ggatgacatg gcgcggccgg tagtggagaa acttcggccc
     4261 cttgtcaaaa aatgccgtgt catactcgtc gccctcgagc ac
//
LOCUS       DASQKS010000129         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5532469, whole genome shotgun sequence.
ACCESSION   DASQKS010000129 DASQKS010000000
VERSION     DASQKS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5532469"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1435
                     /locus_tag="VE170_03345"
     CDS             128..1435
                     /locus_tag="VE170_03345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14496.1"
                     /translation="MSDETPDAPPAKAEDKVARPRGMLGYFGALRIFEALHYREYRLI
                     WFGQIFASMATWMDQVARGWLLYELTNSTLQLGLIRGVQAIPILLLSPLAGSAADRYS
                     RKNQILIAQIVDGLLYVVVAILIFAERIEPWHVFVTAFGMSIVQSFQQPSRAALVGDA
                     VPTEHITNAIGLNSIIFNVARSAGPAFAGFLIALVGTGGAYTAQAICYFLATIWTWQL
                     RVGQTPPKITQDKHGHDESVGQSIIEGWKFSWRNEAVRASLLIVICASIFIIPFMTLL
                     PVFARDVLHVGATGQGLLLSAMGIGALSSSVLIASFGDRVPRINIMLGGVTMYGIIIA
                     AFALSPWFKLSVLLMGLVGVVHVTSHALAQTVIQTYSPKEFRGRTMALYHMTHVILLL
                     GGILIGALAARIGAQWATAMLSLAGTLSMGIIYLALPRARLIR"
     gene            1579..2607
                     /locus_tag="VE170_03350"
     CDS             1579..2607
                     /locus_tag="VE170_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14497.1"
                     /translation="MNVSLRRLAVLAFLAASLLCTHPAGGANPKLVEAARKEGKLVAY
                     VSMLTENATALLAEFKKKYPFIDTSLYRANTQKLLPKIQLEARTQQHEADVISATFTI
                     WNELTRGKLVMKYDSPERAKYPETLKDADGYWNILHLGVQGMAYNTKLVSPDSAPKKY
                     EDLLHPRWRPKQIAMDYRDSSWMAVMLEIMGEAEGLAFMKKLAAKDLYMRENKNLLTQ
                     LLAAGEFPVLANTYLETFAKIQKAGAPIEWVPGRNPIPASTHLLGVYAHARHPNAAKL
                     FVDFLLSPEGQSLTANVIGSYPANPNVESDLRKKTAGYRLHPVNPKMMARFDEVNKQY
                     MEIFWKNP"
     gene            complement(2813..3379)
                     /locus_tag="VE170_03355"
     CDS             complement(2813..3379)
                     /locus_tag="VE170_03355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ14498.1"
                     /translation="MKLLRTAILAIVVCTTLQSPVDSQESIPYVPSTMPVVAKMLDLA
                     SVTKDDIVYDMGCGDGRILIMAAKKYGARGVGVDLDPVRIAEAKKNAEQEGVSHMVEF
                     RAEDGTTTDISKATVVTLYMFKWFNNAISPKLQKLRPGSRVVAHDFDIDDWKPTSVVN
                     LDAERNGHEGIERPHTLYLWLVGPEQKA"
BASE COUNT          717 a         1068 c          946 g          733 t
ORIGIN      
        1 tatcgccagt taacgcgggc gtaaatgacg aaaatcagcc atcggcggtg catggcgtga
       61 agcttggcgt agtcaataca gctggacggc ggtcgagcgg cagcgtagcc ggattacgca
      121 tcccggcatg agcgacgaaa cgccggatgc gccgccggcc aaagccgaag acaaagtcgc
      181 gcggccgcgc ggtatgcttg gctacttcgg cgctctgcgc atcttcgaag cgctgcatta
      241 ccgcgaatac cggttgatct ggttcggcca gatcttcgcg tcgatggcca cctggatgga
      301 tcaggtcgcg cgcggctggc tgctctacga actgaccaac tcgaccctcc aattggggct
      361 catccgcggc gtgcaagcga tcccgattct gctgctttcg cccctggccg ggagcgcggc
      421 ggaccgctac tcgcgcaaaa atcagatcct gatcgcccaa atcgtcgacg gcctgctcta
      481 tgtcgtcgtg gcgatcttaa tttttgccga gcgcatcgag ccctggcatg tttttgtcac
      541 agccttcggc atgtcgatcg tgcagtcgtt ccagcagccg tcgcgcgccg ccttggtcgg
      601 cgacgccgtg ccgacagaac atatcaccaa cgccatcggc ttgaattcga ttattttcaa
      661 cgttgcgcgc agcgccggcc cggcgttcgc gggctttctc atcgccctcg tcggcaccgg
      721 aggcgcctat acggcgcaag cgatttgtta ctttctcgcc acgatctgga catggcaact
      781 gcgggtcgga caaactccgc caaagatcac ccaggacaaa catggccacg acgaatccgt
      841 cgggcaaagc atcattgagg gatggaagtt cagttggcgc aacgaggcgg tgcgcgccag
      901 cctgttgatc gttatctgcg cgtcgatctt catcattccg ttcatgaccc tattgccggt
      961 cttcgcccgc gacgtactcc atgtcggcgc caccgggcaa ggactgctgc tcagcgccat
     1021 gggcatcggc gccctgtcca gctcggtgct gatcgcatcc ttcggcgatc gcgtgccgcg
     1081 gatcaatatc atgctgggcg gcgtgactat gtacggcatc atcatcgccg ccttcgcgct
     1141 ctcgccatgg ttcaaactat cggtgctgtt gatggggctg gtcggcgtcg ttcatgtcac
     1201 ctcccacgcc ctggcgcaaa ctgtcatcca gacctactcg cccaaggaat ttcgcggccg
     1261 caccatggcg ctctatcaca tgacccacgt gatcctgctc ctcggcggca tcctcatcgg
     1321 cgcgctggcc gcacggatcg gtgcccagtg ggccacggcg atgctcagcc tggccggcac
     1381 tctcagtatg gggatcattt acctcgctct gccgcgcgcc cgattgatcc gttagaacag
     1441 tccccatcgt tgaaaattaa tttatcgcct tgacagtgcc gcgccttcgg ctaaagaatt
     1501 accgcatgaa ctctcagggt cggccaatgg cttgatccat tcggccatgg aactcatagt
     1561 cctcgcgagg gcatgctcat gaatgtatcg ctacgccgcc ttgcagtttt ggcatttttg
     1621 gccgcttccc tgctgtgcac tcatccggcc ggtggggcca atccgaagct cgtcgaagcg
     1681 gcgagaaaag agggcaagct ggtcgcctac gtcagcatgc tgacggaaaa cgccaccgct
     1741 ctgctcgccg agttcaagaa gaaatatcca ttcatcgaca cttcgctcta tcgcgccaac
     1801 acgcagaaac ttctccccaa gattcaattg gaagcgcgca cacaacagca tgaagccgac
     1861 gtcatcagcg cgacgtttac gatctggaac gaactcaccc gcggcaagtt ggtcatgaaa
     1921 tacgattcgc ccgaacgcgc caaatatccc gagacgttaa aagatgccga cggctactgg
     1981 aacatcctgc acctgggggt ccagggcatg gcttacaata ctaaattagt ttcgccggac
     2041 agcgcgccga aaaagtatga agacctgctg catccgcgct ggcggcccaa gcagatcgcc
     2101 atggactacc gcgacagttc ctggatggcg gtgatgttgg aaatcatggg cgaagcggaa
     2161 ggtctggcgt tcatgaaaaa gctcgcagct aaggacctct acatgcgcga gaacaaaaat
     2221 ctcctcactc aactattggc cgccggtgag ttcccggtgc tggcgaacac ctatctggaa
     2281 accttcgcga aaatccaaaa agccggcgcg cccatagaat gggtgccggg gcgaaatccg
     2341 attcccgcgt cgacccactt gctcggggtc tacgcccatg cgcgtcatcc caacgccgcc
     2401 aagctgttcg tcgacttttt actatcaccg gagggccaga gcctaaccgc caatgtcatc
     2461 ggcagttacc cggccaaccc caacgtcgag tcggacttgc gcaaaaaaac cgccggctac
     2521 cgcctccatc cggtcaatcc aaaaatgatg gcgcgcttcg acgaggtcaa caaacagtac
     2581 atggaaatct tctggaaaaa cccttaagta cgccacgggc gaaggcgttt gtcagccgat
     2641 gtgaatttat cccggcggac tgcgtttgga tcgggcgggc gaaacgcggc ctttttgaag
     2701 ttctcgatcg gcaacgatga cgccttcgag acccatgcct gatcgtgatt tgcgagaacc
     2761 gttggtaaag tttgcgccgc cgaaggcatt gaacgattgt cttacgcggc ggctaggcct
     2821 tttgttcagg accgacaagc cataggtaaa gcgtatgcgg ccgctcgata ccctcatggc
     2881 cgtttctctc ggcgtcaagg tttaccaccg acgtcggttt ccaatcgtcg atatcgaagt
     2941 cgtgggcgac cacgcgcgaa cccggacgca acttttgcag cttggggctg atcgcgttgt
     3001 tgaaccattt gaacatgtag agcgtcacca ccgtcgcctt ggaaatatcg gtcgtggtgc
     3061 cgtcttcggc gcgaaactcg accatgtggc tgacgccctc ctgttcggcg ttcttcttcg
     3121 cttcggcgat acgcaccggg tcgagatcga cgccgacgcc gcgagcgccg tattttttcg
     3181 ccgccatgat caagatccgt ccatcgccgc aacccatatc gtacacgatg tcatccttgg
     3241 tcaccgacgc gagatcgagc atcttggcca ccaccggcat ggtgctgggc acgtaaggaa
     3301 tcgactcctg agaatcgact gggctctgaa gcgtcgtgca aactacaatc gccaatatcg
     3361 ccgtgcgtaa taatttcatc cgttcacctc gctgttgcta aagctagaac catttctcgc
     3421 tgccaaattg actatgtgca ttctcacatt tccccaagct tggc
//
LOCUS       DASQKS010000130         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4271257, whole genome shotgun sequence.
ACCESSION   DASQKS010000130 DASQKS010000000
VERSION     DASQKS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4271257"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..741
                     /locus_tag="VE170_03360"
     CDS             280..741
                     /locus_tag="VE170_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14499.1"
                     /translation="MAEFKAVGASVPRAEAGDKVSGQSIYAADVKLPGLLWAKILRSP
                     HPHARIVHVDSSKAAKVPGVKAILTGADVRGLLIGKQIRDLPVLCWDKARYVGDRVAA
                     VAAETVDAAEEAIQLIDVEYEILPAVFDPLEAMAPDAPQLHDNVAAYDGAP"
     gene            832..2511
                     /locus_tag="VE170_03365"
     CDS             832..2511
                     /locus_tag="VE170_03365"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ14500.1"
                     /translation="MFEHTFNIPARHQGYLEPHAATVAIEAGGRVQVWAGVKNPFGVR
                     SQLSKCLNLPEERIRMNVVNVGGEFGGKGDGIDLPILYFLAQKTGRPVKFIMTYAEEL
                     SASNPAHPTVITIKSGVMRDGRLVARKMRAVHASGAYGALKSNTSLATWHYAGGQYLI
                     EHADIEFMQIYTNTVPGGYYRSPGAVATAFAVDCHTDIIAKELGIDAAEIRLMNFLSD
                     GETDAVGQELKDVRFREVLQGALDAAGWKKRKKANHGRGIALSGRHISGGDTGVILSV
                     DADGYLIVSPSIDQGSGTHTILRQLVADRMKIGIDRVRVIIGDTDTAPHDGGMRASRM
                     TYVAGNAMITACDQLREKLLAEAARILESRKEEIEFDGAKFFVRQDPGQQVPMRRVVA
                     QTTEPLLITVYEDYRAPDEFSYICAQVAEVEVDPETGAVRLHSFVTAHDVGTIINPLT
                     HQGQIDGAAVMGVGQGLMEEIVIDQGKVTNSNLGDYKLPSIADLFELKTVLVPSGGGV
                     GPHNAKPIGEFANNAPPAAIANAIADAVGVRLFNLPVRAETIYRALRAHQH"
     gene            complement(2611..2946)
                     /locus_tag="VE170_03370"
     CDS             complement(2611..2946)
                     /locus_tag="VE170_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14501.1"
                     /translation="MNKMSYAAVVILTLLIIGCGSSNDGAFAARSGREAQQNERSAIV
                     QRLIPEEGALPENRLKDVLAAISMVDKGFEMENPTRERVRRHLLDREVTTLRRIETNV
                     RAIERKRRN"
     gene            3189..>3385
                     /locus_tag="VE170_03375"
     CDS             3189..>3385
                     /locus_tag="VE170_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14502.1"
                     /translation="MACLRYGITLLFILANLVGVGSAQTKKPGTLSELVAYTGADREK
                     ILIAGARAEGKVTWYTSLAGSS"
BASE COUNT          698 a          976 c         1017 g          694 t
ORIGIN      
        1 ccttggtact actgtcggtg atagcaaatt tctcgatgtg ttaaaggcaa gagggcgggt
       61 ttgaaacccg cccctacacc tgtcgaatct tttttgtgcc ctttgcattc tttccggccg
      121 acgttcttgt tcggtcgcgg ctccatgttc gcgtcactct gataaccaca gtcctctgcc
      181 gcaccattaa tgctttgcca ccttgacctt gggtgctccg gcgtactaat tacgctgaag
      241 gccgattagc cgttcaaata tattaaggaa acaaacacca tggctgaatt caaagcggtt
      301 ggagcgtcgg tgcctcgggc ggaggcgggt gacaaggtca gcggtcagtc gatatatgcc
      361 gccgatgtga agctgcccgg actgctctgg gcgaagatct tgcgcagtcc gcatccgcac
      421 gcgcggattg tccatgtcga tagcagcaag gcggccaagg ttcctggcgt gaaggcgatt
      481 ctcaccggcg cggatgtgcg cgggctgttg atcggcaagc agattcgcga cttgccggtg
      541 ctgtgctggg acaaagcgcg ctacgtcggc gaccgggtgg cggcggtggc ggcggagacc
      601 gtggatgccg ccgaggaagc cattcaatta atcgatgtcg aatacgaaat tcttcccgcg
      661 gtattcgatc cgctggaagc gatggcgccg gacgcgccgc agctccatga caacgtcgcc
      721 gcgtacgacg gcgcgccgta aaatattctc gctacctacc tgcgcaacgg tcttacccgg
      781 ttagcgtgga agaaaggcga catcgagcaa ggcttccgcg acgccgatcg gatattcgaa
      841 cacaccttca atataccggc gcgccatcaa ggttacctcg aaccgcacgc ggcgaccgtc
      901 gccatcgaag cgggcggccg cgtacaagtt tgggccggag tgaaaaatcc gttcggggtg
      961 cgcagccagt tatccaaatg cctgaatttg cccgaagagc gtatccgcat gaacgtggtc
     1021 aacgtcggcg gcgagttcgg cggcaaaggt gacggcatcg acttgccgat cctctatttt
     1081 cttgcgcaga aaaccggacg gccggtgaag ttcatcatga cctacgctga ggagctgtcg
     1141 gcaagcaatc ctgctcatcc gaccgtgatc acgatcaaaa gcggggttat gcgcgacggc
     1201 aggctggtag cgcggaaaat gcgcgcggtc catgccagcg gggcctacgg cgcgctcaag
     1261 tccaatacct cgttagcgac ctggcactac gccggcggcc agtatctcat cgagcacgcc
     1321 gacattgagt tcatgcagat ctataccaat accgtgcctg gcggttatta ccgaagtccc
     1381 ggcgcggtag cgacggcgtt cgcagtcgac tgccacactg acatcatcgc caaagaattg
     1441 ggcattgatg cggccgagat ccgcctaatg aactttctta gcgacggcga aaccgacgcg
     1501 gtcggtcaag aattgaaaga cgtccgcttt cgtgaagtgc tccaaggcgc actggacgct
     1561 gccggttgga agaaacggaa gaaagccaat catggccgcg gcattgcgct ctcgggccgc
     1621 catatcagcg gcggcgacac cggcgtgatt ctcagcgttg acgcggacgg ctacttgatc
     1681 gtcagtccga gcatcgatca aggctcgggc acgcacacga tcttgcgcca gttggtcgcc
     1741 gaccggatga agatcggcat cgatcgggtg cgcgtgatta tcggcgacac cgacacggcg
     1801 ccccacgatg gcggcatgcg ggcgagccgc atgacttacg tcgccggcaa tgcgatgatc
     1861 accgcctgcg atcagttgcg ggagaagctg ctcgccgagg cagcgcgcat cttggagagc
     1921 cgcaaggaag aaattgaatt cgacggcgct aagtttttcg tgcgccaaga tcccggccag
     1981 caggtgccga tgcgccgggt tgtcgctcaa acgacggaac cgctgctcat caccgtctat
     2041 gaagattatc gcgcacccga cgagttttcg tacatctgcg cccaggtcgc cgaggtcgaa
     2101 gtcgatcccg agaccggcgc ggtgagactc catagcttcg tcaccgcgca cgatgtcggc
     2161 acgatcatca atccgttgac acatcagggg cagatcgacg gcgcggcggt gatgggcgtc
     2221 ggccaggggc tcatggaaga gatcgtcatc gatcaaggca aggtgaccaa cagcaatctg
     2281 ggcgattaca aattgccgtc catcgccgat ctgttcgagt tgaaaaccgt tctcgtcccc
     2341 tccggcggcg gcgtcggccc gcataacgcc aagcccatcg gcgagttcgc caacaatgcg
     2401 ccgccggcgg ccatcgccaa cgcgatcgcc gatgcggttg gcgtgcgctt gtttaatctg
     2461 ccggtaagag ctgagacgat ttatcgcgcg ttgcgagccc atcaacacta aacagctggc
     2521 atgggtcgcg accggctagc gtgcattgtc agtcgacccg gcggagcgga atatggaaag
     2581 gggggcgcgg gatgtaaaat tcgcgtcggt ctagttcctg cgtttccttt cgatggctcg
     2641 gacgttggtc tcgatgcggc gcaacgtcgt cacctctcgg tctaacaggt gacggcgaac
     2701 tctttcccgt gtgggatttt ccatctcgaa ccccttgtcg accatggaaa tcgcggcaag
     2761 cacgtccttc aagcgatttt ccggcagcgc accctcctcg ggaatcaaac gctgcacgat
     2821 cgccgaccgt tcattttgtt gcgcttcccg cccgctgcgc gcggcgaaag ccccatcgtt
     2881 actggagcca cagccgatga tcagcagggt cagaatgacg acggctgcat aactcatttt
     2941 attcataggc gcctccgtga cagtattgtc ttagccgcga gtcgacgttg tttgctcttt
     3001 ttcgcttata cgatggctgg tcgattctca accgcgcaat agctcgtacg gattagacgg
     3061 aaatgaccgg agggcattca cggccaccgt gattttcccg cgccgcaatc gagaaagttg
     3121 atttgtagga accgcgcgtt tgcggtaggt tcaagaactt ttcatctaac cggaggttgt
     3181 aggacgccgt ggcatgctta cgatatggaa tcacgctttt attcatctta gccaatttag
     3241 ttggcgtggg atcggcgcaa accaagaaac ccggcacgct gtctgagctg gtggcctata
     3301 ccggcgcgga tcgggagaag attcttattg ccggcgccag ggccgagggg aaagtcacct
     3361 ggtacacctc gctggccggc agctc
//
LOCUS       DASQKS010000131         7608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2604647, whole genome shotgun sequence.
ACCESSION   DASQKS010000131 DASQKS010000000
VERSION     DASQKS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604647"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VE170_03380"
     CDS             complement(<1..699)
                     /locus_tag="VE170_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14503.1"
                     /translation="MLKTIFGVAVIMVLSVATHLTAQPIRMTIGQTGINPGTAPFIIA
                     QKENFFAKYGIEVKIIETQTTAAVQAMLGGSMQFTMGAGAAFTTATLEGAPPFVNVAS
                     WINVFPYYLVARKEFAKVADLKGKLGQVGGPFGAAPDVAMRFGLSKLGIDPEKDVKFV
                     QMGRPDWANVIVQLEKGDVQFSVLPPPFDRIAETRGIHKLYALPDLGIQWQQNGEWVL
                     KSYLASNRDAVVRIE"
     gene            1189..2217
                     /locus_tag="VE170_03385"
     CDS             1189..2217
                     /locus_tag="VE170_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14504.1"
                     /translation="MASFLWIFLLTETNFLSAQEGYFKGKTIRIIAGFPGGGGVDAEA
                     RMMARFLGRYIPGYPTMLVQNMPGAGGTVASNWFEQFAKPDGLTLHYTSTTSINQQAM
                     SVKEAKHDLTRWPAIGSVRRGTSVALIRKKSLDRLTGGGEPLKVGVRSGDESWNAIFL
                     WGAEFLKWNVRWVQGYPGGGEILLAFERNETDIYPTATLPTLQKLIGQGFTPFVQQGM
                     LGANGLFQRRSEFPDVPVFEDVAKEQRPSGVPWQAYTILAGTDSVGRPLHAAPRTPAN
                     LLHQLRDGFTRMKDDAEFKLELKRVSGDDAQMLSAAETEQLLRQLLVVSPSIQGYING
                     LMKKYLNR"
     gene            2489..3502
                     /locus_tag="VE170_03390"
     CDS             2489..3502
                     /locus_tag="VE170_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14505.1"
                     /translation="MLSFRTVLLVLLAIPGGVVAALAAVASNKMTIAYASIGPRSAPL
                     WAADEYGIFRKYGIEPQVIFVRGAPTLVAALTSGDMDIGYTGGTAVLGASVAGIDLKI
                     LAVLTNRVTYDLVARPGIKSAEDLRGKRFGVTSIGGTLWMGGVLALEKLGIDSSRDDI
                     RFLVLGDQVVLAQALEDNRIDATVLDMVFSKRLAQKGFPILAELHKTNLPITSTSIVA
                     RQGYIQKNSQLMENFMKAVMEGIAFVLGPVNKAATIKLLQRRLHVSEREAEEGFVDML
                     IGMDKKPFPSMEGLRNIQRLMKQRNPKMANIKVEDLVDDYILRRLDESGFIDKLFAAH
                     GVK"
     gene            3600..4208
                     /locus_tag="VE170_03395"
     CDS             3600..4208
                     /locus_tag="VE170_03395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HYJ14506.1"
                     /translation="MIELNPESTGLVIVDMQVEGCERHGPEMKPVIANIRALLDRFRA
                     IDGKIIHVQSVRTKDHPEFTVFGRPYGLILGSPGADFVEELRPLAGETVVQKTSHDCF
                     YKTSMETALDKLDLRACRDTVIVTGIGSSNCVYHAVIGFHIRNYYTIVPEDCIHGVAA
                     HSQPFALAQFRSNAYNFNVTVSRSDQLQLDAAPVPRRGIARA"
     gene            4310..5221
                     /locus_tag="VE170_03400"
     CDS             4310..5221
                     /locus_tag="VE170_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14507.1"
                     /translation="MKVPYAALSPTYAPLWIAEQAGYFKKYGLEIQPVYISAGSIIVP
                     ALLSGQVEIANMSSAPALTAWARGAELVAVGVTSNRLLHVVMARGAIKKPEELKGKRV
                     GSDRYGSLSDLVLRDALRHFNLTPDRDVAIMQTGGLPERLGALKAGAVDGAILTGDTA
                     FHAEKLGFHTVIDLSQLPIQYPGSTIVVSKALVQSKRDVLKRFLRGWIEGIKTARTDR
                     ELTVKVLQKFLKTSDREILDRTFEVYKDVQEKIPVPDAKVMAVALKQLAATVPQAGQL
                     KAEDFIDRSLIGELESEGFIAKLYGGR"
     gene            5314..6375
                     /locus_tag="VE170_03405"
     CDS             5314..6375
                     /locus_tag="VE170_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14508.1"
                     /translation="MTAKKNLAILFSAILFVPTVAPPAIYSQAPFFQGKTIKIINNDP
                     GGTAGLRVKVVMTHLRKYIPGNPTLLIEFVEGGGGRRAANQVFQNAKPDGLTVGALSS
                     TIIGLQVMKETGVMYDIDKFIYLGTPVSENHNVIYTRRELGLNSLDKLRAASGLRLGA
                     QEVGAVSYISARLFGYFLDLKAPKFVTGYSSLEADVALRNGEIDSRSNNVTSVLSRNR
                     DWIDKGVMDFHAVIAVPKDDKPAHPRLAQLPEIESFAKTDRERKLLTMWRSFRTATTP
                     FVLPPGTPKDRVEILQDAMRKVFKDPEFRAEFKKLANDDASPLMPEELAKVIQDMPRD
                     IEVVDLLKKFSGVEPLPVR"
     gene            6420..7505
                     /locus_tag="VE170_03410"
     CDS             6420..7505
                     /locus_tag="VE170_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14509.1"
                     /translation="MSNHSLRLCLFVVGVNVAWVAQSYAAEKPFYEGKTLTVLINYAA
                     GGPTDVEGRLLARHLGRHVAGQPNVIVQNISGAGGIIGTNFLGQVAKADGLTMGYFTG
                     ALFHQQVRNPALRTDLSKYAFISGIQGVTVSYIRSDVAPGIKKSGDLLKAQRFRAGGL
                     SNDSFKDVAFRLSFDLLGLQYDYVTGYNSSADARLAVQRNEVQYHDETLPAYRSQVEP
                     NMVKPGQVTALYHADLIDADGGVADSPDVPELPSFTKFYTQLLGKPPSGIKYEALKAA
                     NIAGTNMTRAILLPPGAPKEAVAALKQAAASLSRDEEYLADALKVMRFRPRFEMGASG
                     ERLYQRATGISPDLAKFIRQFIEQERK"
BASE COUNT         1665 a         2079 c         2249 g         1615 t
ORIGIN      
        1 ctcaatccgc accacggcat cgcgattact ggctaaatag gatttcaata cccactcgcc
       61 gttttgctgc cattgaatgc ccaaatcggg cagcgcgtaa agtttgtgaa tgccgcgcgt
      121 ctcggcgata cgatcgaagg gcggcggtag caccgagaac tggacatcgc ctttttcgag
      181 ttgaacgatc acgttggccc aatcgggcct ccccatctgc acgaacttca cgtctttctc
      241 gggatcgatg ccgagcttgg atagaccgaa acgcatggcg acatcgggcg cggcgccgaa
      301 cggtcctccg acctggccga gcttgccttt gagatcggcg accttggcaa actccttgcg
      361 cgcgaccaga taatagggaa agacgttgat ccacgatgcg acattgacga agggcggcgc
      421 accttccaag gtcgccgtgg taaatgccgc gccggcgccc atggtgaact gcatgctgcc
      481 gccgagcatc gcctgcaccg ccgcggtggt ttgcgtctcg atgatcttga cttcgatgcc
      541 gtacttggcg aagaagtttt ctttctgcgc aatgataaac ggcgcggtgc cgggattaat
      601 accggtctgg ccgatggtca tcctgatcgg ctgggcggtg agatgagttg ccaccgacag
      661 caccatgata acggctacgc cgaatatggt tttcaacatg ggtggtctcc ttcgattttt
      721 gccggatcat atttccgcgc gtgcgaagct gtcaaggaga agtaggtaag gataaacgct
      781 tacggatgac ggactacgga cgatgcgcac actgggttat ttacgccgtc atgcagcgtt
      841 atcgacacta ggaagaattt ttttatacac ctcgttgaat taaaaatctc gaccggttct
      901 cacggcgaat aatattcgga aagcagtctg caaggtgaag atcgattgga taggattctc
      961 acagcaacaa atgcgttcga gcagcttgcc tcgtctcacg gattcgggct aaggatagaa
     1021 tgacggttgc ccatgaatgt tggaggaaga gaaaagcctt ttgatcgaat caggcttcta
     1081 accccgcact ccccggcggg cttcgctgcg gcgcagacag agtttccgct aaagctcaag
     1141 tattcatagg gagaggattc atctatgcgg attaaaccta gctataggat ggcttcattc
     1201 ctatggattt tcttacttac cgagaccaat tttttgtccg cccaggaggg ctatttcaag
     1261 ggcaagacca ttcgcatcat cgccggtttt cccggcggcg gcggggtcga tgccgaggcg
     1321 cgcatgatgg cgcgtttcct gggacgttat attccaggct atccaacgat gctggtgcaa
     1381 aatatgcctg gggcgggtgg aacggtagcg agcaattggt ttgagcagtt cgccaagccc
     1441 gacggcttga cgttgcacta caccagcacg acgtcaatca atcagcaggc catgagcgtc
     1501 aaagaggcca agcatgattt gacccgctgg ccggcgatcg gcagcgtgcg gcgcggcacg
     1561 tcggtcgcgc tgatccgaaa aaaaagcctc gatcgtttga ccggcggggg cgagccgctc
     1621 aaagtcggtg tccgttcggg cgacgaaagt tggaacgcga tctttctttg gggcgcggaa
     1681 tttctcaaat ggaatgtccg gtgggtgcaa ggttatcctg gcggcggtga aattctgctt
     1741 gccttcgagc gcaatgaaac cgacatctat ccaaccgcga cactgccgac gctacagaag
     1801 ctcatcggcc agggcttcac gccgtttgtg caacagggca tgttgggcgc caacggtctc
     1861 ttccaaaggc gcagcgagtt cccggatgtg ccggtgttcg aagacgtggc caaggagcaa
     1921 cggccgagcg gtgtgccgtg gcaggcctat accatattgg ccggcaccga cagcgtcggc
     1981 cggccgctgc atgccgcgcc aaggaccccg gcgaacttgt tgcaccaact gcgcgacggc
     2041 tttacgcgca tgaaggatga cgccgagttc aaactggagc tgaaacgggt gagcggcgac
     2101 gatgcgcaaa tgctttcagc cgccgaaacg gagcaactgc tgcgccagct cctggtcgtg
     2161 tcgccgtcga tccaaggtta catcaacggc ctgatgaaga aatatttgaa taggtagaat
     2221 ttcgggtcgg cgggacgagc ggattattgg ccgcaaagaa cgcaaagaag tcagagaatt
     2281 gtcgggtgcg ctccgcgctc cggtcagtcg cgctttactt tcctcgtaag aatgcaaatt
     2341 cgcatttccg ttcttgcgcc ctctgcgact gtaacgcgtt cgagtgcggc aaaccttttc
     2401 gacgccgttg acagcaacaa atactctcga ctactgtccg caaaggcacg catcggcgca
     2461 ggaactgtcg tagcgaaagg agagagccat gcttagcttt cgaacggtgt tgctagttct
     2521 gttggcgatt ccgggcggcg tggtggcggc cctggcggcg gtcgcgtcca acaagatgac
     2581 gatcgcgtat gcttccatcg gcccgcgctc ggcgccgctc tgggctgccg acgagtatgg
     2641 catctttcgc aaatatggca tcgagccgca agtgattttt gtgcgcggcg caccgacgtt
     2701 ggtggcggcg ctgacttccg gcgacatgga tatcggttac accggcggca cggcggtact
     2761 cggcgcttcg gtggcgggca tcgacttaaa aattctcgcg gtcctgacca accgggtgac
     2821 ttacgacctg gtggcgcgac cgggcatcaa aagcgccgaa gacttacgtg gcaaacgctt
     2881 cggcgtcacc agcatcggcg gcacgttgtg gatgggcggc gtgctcgcct tggaaaagtt
     2941 gggaatcgac agcagccgcg acgatatccg tttcctcgtt ctcggcgacc aggtagtcct
     3001 cgctcaagcg ctggaagata atcgcatcga cgccactgtg ctcgatatgg tttttagcaa
     3061 gcggctcgcc caaaaggggt ttccgattct agccgagctg cacaagacca acctgccgat
     3121 caccagcacg agcatcgttg cgcgccaagg ttacattcag aaaaattccc agctcatgga
     3181 gaattttatg aaggcggtga tggagggcat cgccttcgtg cttggcccgg tgaacaaagc
     3241 ggccacgatc aagttgctgc agcgacgtct gcatgtgagc gaacgcgagg ccgaagaagg
     3301 ttttgtcgac atgctcatcg gcatggacaa gaagccgttt ccgtcgatgg aaggcttgcg
     3361 caatattcag cgcttgatga aacagcgcaa cccgaaaatg gccaacatca aggtggagga
     3421 cttagtcgac gattacatcc tgcgccgcct tgacgagagc gggtttatcg acaagttgtt
     3481 tgccgcgcat ggggtgaaat agcggttgcc gcaaccctga cattgacaag gcgaaagacg
     3541 ctatgaaata ttcggccgga ccgacaatcg gcctcggcag gaaattggag agcaatacca
     3601 tgattgaatt gaaccccgaa tcgactggat tggtgatcgt cgacatgcaa gtggagggct
     3661 gcgaacgcca tggccccgag atgaaaccgg tcatcgccaa catccgcgct ttactcgatc
     3721 gcttccgggc gattgacggc aagatcattc atgtgcaatc ggtgcggacc aaggatcatc
     3781 cggaattcac cgtattcggc cggccctatg gtttgatcct gggcagcccc ggcgccgatt
     3841 tcgtcgagga attaagaccg ctggcgggtg agacggtggt gcagaagact tcgcatgact
     3901 gcttctacaa gacgagcatg gaaacagcgc tcgacaagct tgacctgcgc gcctgccgtg
     3961 acacggtgat cgtcaccggc atcgggtcga gcaactgcgt ctatcacgcg gtgattggat
     4021 ttcacatccg caattattac acgatcgtgc cggaagattg cattcacggc gtcgccgcgc
     4081 actcccagcc tttcgcgttg gcgcagttcc gctccaacgc gtacaacttc aacgtgacgg
     4141 tgtcgcgatc cgaccagctg cagttagatg ccgcgccagt cccgcggcgt gggattgccc
     4201 gggcttgaaa gctcgaccga ttatacagtt gcgacgcaag ctcggattac tcactgttct
     4261 ggtttcagtc tgtttgctgc cgatgccgcc gtcgctcttc gcgcagagta tgaaagtgcc
     4321 ctatgcggcg ctgtcaccga cttacgcgcc gctgtggatt gccgagcagg ccggttattt
     4381 caaaaaatac ggcctggaaa tccagccggt ttatatctct gcggggtcga tcatcgtgcc
     4441 ggcgctgtta tccgggcagg tggagatcgc caacatgagt tcagcccccg ccctcaccgc
     4501 gtgggcgcgc ggcgccgaac tggtggcggt gggtgtgact tccaatcgtt tgcttcatgt
     4561 cgtcatggcc cggggcgcga tcaagaaacc ggaggagctg aaaggcaaac gggtaggtag
     4621 cgaccgctac ggatcgcttt ccgatttagt gctgcgggat gcgttgcgcc acttcaattt
     4681 aactcccgac cgcgatgtcg cgatcatgca gaccggcggt cttcccgaaa ggctcggcgc
     4741 gttgaaagca ggcgccgtgg acggagcgat tctcaccggc gacacggctt ttcacgcgga
     4801 aaagttaggc ttccatacgg tgatcgatct tagccagctg ccgattcaat atcccggcag
     4861 caccatcgtg gtgagcaaag cgttggttca gtccaagcgc gacgtgctga aaagatttct
     4921 gcgcggttgg atcgaaggca tcaagacggc gcgcaccgac agggagttga ccgtcaaagt
     4981 gttgcaaaaa tttcttaaga catccgaccg ggagatcctc gaccgcacct ttgaggttta
     5041 caaagacgtg caagaaaaga ttcccgtccc ggacgcaaag gtaatggcgg tggcgttgaa
     5101 acaactcgct gccacggtgc cgcaagccgg ccaactgaag gcggaagatt tcatcgaccg
     5161 gagtctaatc ggcgagctcg aaagcgaggg gtttatcgcc aagttgtacg gcggcaggta
     5221 ggaaccaatc gatcactcat cggagcaatc ggctttgtcc cgtcggctag tggaaactga
     5281 tggtcaggcg atgcttgaat cctggaggtg ctcatgacag cgaaaaaaaa ccttgcgata
     5341 ttattttccg ccatcttgtt tgtgcccacg gtcgcgccgc cggcgattta ttcccaagcg
     5401 ccattctttc agggcaagac gatcaagatc atcaacaacg atcccggcgg taccgcgggc
     5461 ttgcgggtca aggtggtgat gacccacctg cgcaaatata ttcctggtaa tccgaccttg
     5521 cttatcgaat ttgtcgaagg cggcgggggt cgccgggcgg ccaaccaggt ttttcaaaac
     5581 gccaagccgg acggcctcac cgttggcgcg ctcagcagca cgatcatcgg ccttcaggtg
     5641 atgaaagaga ccggcgtcat gtacgacatc gataagttca tctatctcgg cactccggta
     5701 agcgagaacc ataatgtcat ttacacgcgg cgggagttag gtctcaattc actcgacaaa
     5761 ctgcgcgcgg cgagcggcct tcgtctcggc gcgcaagagg tgggcgcggt gtcgtacatc
     5821 tcggcgcggc tgttcggtta ttttctcgat cttaaagcac ccaagttcgt caccggctac
     5881 agttcgttgg aagccgacgt ggcgttgcgc aatggcgaga tcgattcgcg ctcgaataac
     5941 gtgacttcgg tgttgagccg caatcgcgac tggatagaca agggcgtcat ggactttcat
     6001 gcggtcatcg ccgtgcccaa agacgacaag ccggcccacc cgcgcctggc gcaacttccg
     6061 gagatcgaga gcttcgccaa aaccgataga gaacgaaagc tgctgaccat gtggcgcagc
     6121 tttcgaacgg cgacgacgcc gttcgtgctg ccgccgggaa ccccgaagga tcgggtggag
     6181 atcctgcaag acgccatgcg caaggtgttc aaggatcccg aattccgcgc tgagtttaag
     6241 aagctcgcca atgacgatgc ttcaccgttg atgcctgaag aacttgccaa agtgattcag
     6301 gacatgccgc gcgatatcga ggtcgtcgat ttgttgaaga agttctccgg cgtcgagcct
     6361 ctgccggtgc gataaactgc gagcagcggg aaatcggacc cgtaaaaaag gagtagggaa
     6421 tgagtaatca ttcattacgt ctttgcttgt tcgttgtcgg cgttaatgtt gcgtgggttg
     6481 cgcaaagtta cgccgccgag aaacccttct acgagggcaa gaccctcact gttttgatca
     6541 attacgccgc cggcgggccc accgatgtcg aaggcaggct tttggcgcgc catctcggca
     6601 ggcatgtggc gggacaaccg aacgtcatcg tgcaaaatat ttccggcgcg ggcggcatca
     6661 ttggcaccaa ctttttgggc caagtcgcca aagccgacgg gttgacgatg ggctatttta
     6721 ccggcgcgct gtttcatcaa caggtgagaa atcccgcgtt acgcacagac ttgtccaagt
     6781 atgctttcat ctcgggaatc cagggcgtca ccgtgtctta tattcgctcc gatgtcgctc
     6841 ccggaatcaa gaagtccgga gatctcctca aggcccaacg gtttagagcc ggcggtctta
     6901 gcaacgatag tttcaaggat gttgcgttta gactgtcgtt cgatcttctc ggtttgcaat
     6961 acgactatgt gaccggctac aactccagcg ccgacgcccg cttggcggtg cagcgcaacg
     7021 aagtgcagta tcacgacgaa actctgccgg cgtaccggtc gcaggtggag cccaacatgg
     7081 taaaacctgg ccaagtgacg gcgctctacc acgcggactt gatcgatgct gacggcggtg
     7141 tcgccgatag tccggatgtt ccagagctgc cgtcgttcac gaagttttac acccaattat
     7201 tgggcaagcc gccttccggc atcaagtacg aagctttgaa ggcggccaac atcgccggca
     7261 ccaacatgac ccgggcgata ttactgccgc ccggcgcgcc caaggaggcg gtggccgcgc
     7321 tgaagcaggc cgctgcgtcg ctttctaggg atgaagaata tctcgccgac gcgctgaagg
     7381 tgatgcgctt tcgtccgcgc ttcgaaatgg gcgcgagcgg cgaacggctc tatcaacgag
     7441 ccacggggat ttctccggat ttggcaaaat tcatccggca atttatcgag caagagcgaa
     7501 agtagcctgc gtcagaaaaa ttcatggttc aggttcagtt cggtaagaaa gatttctcgc
     7561 ttcgctcaaa atgacagatc gcgagttgct gtcatgccga acccttcg
//
LOCUS       DASQKS010000132         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3435930, whole genome shotgun sequence.
ACCESSION   DASQKS010000132 DASQKS010000000
VERSION     DASQKS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435930"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VE170_03415"
     CDS             complement(<1..223)
                     /locus_tag="VE170_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ14510.1"
                     /translation="MKILVIGSGGREHALVWKIHQSPRVDKIFCAPGSAAIAELAECV
                     AINPEQIDRLADFAEKEQIDLTVVGPELPL"
     gene            complement(293..1867)
                     /gene="purH"
                     /locus_tag="VE170_03420"
     CDS             complement(293..1867)
                     /gene="purH"
                     /locus_tag="VE170_03420"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYJ14511.1"
                     /translation="MGKIETALISVSDKQGIVEFSRALAAMGVELVSTGGTAALLRDS
                     NIPVKDVAELTGFPEMMDGRVKTLHPKIHGGILALRDNPEHVAKMKEHGIKPIDLVVV
                     NLYPFEATVARGAAFEEIVENIDIGGPSMVRAAAKNHQHVGVVVDPADYDSIVAEMRA
                     NHGALLPATHFRLFCKAFQHTSHYDGAISNYFSSLDQDKKPQRWGQTVNIQVSKLQDM
                     RYGENPHQSAAFYGTTGDAGPSVSRAKQFQGKELSFNNILDADAALSTVLEFADIATV
                     AIKHNNPCGVALSNKSLVDSFRKAKACDPVSIFGGVLAFNRPVDEETALELKDIFLEI
                     VIAPSFTAEAKAVLSSAKRLLNIRLLELDMSQPQTGGCDLRRVRGGMLIQDWDTGKID
                     VRACKVVTERKPTDDEYRALDFAWRVCRHVKSNTIVFASPDQVLGVGAGQMSRIDSTK
                     IAVLRAATHGLDLKGSAVASDAFYPFRDGLDAAADGGAKSIIQPGGSIKDEEVIAAAN
                     EHGIAMIFTGMRHFRH"
     gene            complement(1882..3015)
                     /gene="alr"
                     /locus_tag="VE170_03425"
     CDS             complement(1882..3015)
                     /gene="alr"
                     /locus_tag="VE170_03425"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYJ14512.1"
                     /translation="MITTPTGRPTLCVIDHRALRWNFKQIRGLVGARVKILSMVKADG
                     YGHGAVAVAKTLAGAGSDAFGVATVEEGIELRRARIMQPILVLAGVYLEQLEQFLKNR
                     LTPVIHEVVTLKLLDKRLQRLKTTLPVEIEMDTGMGRIGFPTDATDAWLPVLAKLEAV
                     KLHGVFSHFSDAESANQRYSRKQLKSFRQVLDRLAAAKITHRASHMAKSAALITEPTA
                     HFSMVRPGLILYGMYPALKLKKQIELKPVLSWQTRIIQLKRVPAGTSIGYGRTFVTKR
                     NSLIATLPVGYADGYRRLFSNHAQVLVRGKRAPVVGRVSMDLTTIDVTDIRNIHQGDE
                     VVLLGAQGGAEIGADEIAGWANTISYEIFTSIGARVPRIHTNV"
     gene            complement(3044..3388)
                     /gene="metG"
                     /locus_tag="VE170_03430"
     CDS             complement(3044..3388)
                     /gene="metG"
                     /locus_tag="VE170_03430"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HYJ14513.1"
                     /translation="MEPEATPEKISIDDFKKIELKIATITAAEPHPNADKLLVLHVDL
                     GTEQRQICAGIRNHYTPEQLVGKQIVVVANLATAKLRGLESQGMLLAASSGEQVIFMT
                     PEKPALAGSTVK"
     gene            complement(3471..>3890)
                     /locus_tag="VE170_03435"
     CDS             complement(3471..>3890)
                     /locus_tag="VE170_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ14514.1"
                     /translation="DNLDRLRLTQQVQVMRADLFPEGRASLVVCNPPWLPARPASPID
                     SAVYDEDSRMLLGFLNGLAAHLVPSGEGWLILSDFAEHLGLRSRVDLLAAFANAGLKV
                     MGRIDAKPRHPKASDAGDPLHFARAAEVTSLWRLARQ"
BASE COUNT          762 a         1067 c         1203 g          858 t
ORIGIN      
        1 ttagcggcaa ctccgggccg acgacggtga ggtctatttg ttctttttcc gcgaagtcgg
       61 ccaagcgatc gatttgctcg gggttgatcg ccacgcattc ggctaattcg gcgatggccg
      121 cgctgcccgg cgcgcagaaa attttgtcca cgcgcggact ctgatgaatc ttccagacca
      181 gcgcgtgctc gcggccgccg ctgccgatga cgaggatttt cataattcga ttaggatgga
      241 ggatcgaaga ttgaggatgg ccatcatcca tcctcggtcc actattctcg gcctagtgtc
      301 taaaatgcct catgccggtg aagatcatgg cgatgccatg ttcattggcg gcagcgatga
      361 cttcttcgtc tttgatcgag ccgcccggtt ggataatcga cttggcgccg ccatcggcgg
      421 ctgcatcgag gccgtcgcgg aatgggtaaa aagcgtcgga agcgaccgcg gagcccttca
      481 ggtcgagacc gtgagtcgcc gcccgcagca cggcgatctt ggtcgagtcg atgcggctca
      541 tctggccggc gccgacgccg agcacttggt cgggcgacgc gaacacgatg gtgttggatt
      601 ttacatggcg gcaaacccgc caggcgaaat cgagagcgcg atattcgtcg tccgtcggtt
      661 tgcgttcggt gacgactttg caggcgcgca cgtcaatctt tccggtatcc caatcttgga
      721 tcagcatgcc gccgcgcacg cgccggagat cgcagccgcc ggtctgcggc tgactcatgt
      781 cgagttcgag caagcggata ttgagcagcc gtttcgctga tgagagcacg gctttagctt
      841 ccgccgtgaa gctcggcgcg atgacgatct cgagaaaaat atccttgagt tccaatgcgg
      901 tttcctcgtc cactggacga ttgaacgcca gcacgccgcc gaaaatcgat accgggtcgc
      961 aggccttggc tttgcggaag gagtcgacga gggatttatt cgacagggcg acgccgcaag
     1021 ggttgttgtg cttgatggcg acggtggcga tatcggcgaa ttccaacacc gtgctcaagg
     1081 ccgcgtcggc gtcgaggatg ttgttgaacg acaattcttt gccttgaaac tgtttcgccc
     1141 gcgagaccga tgggccggca tcccccgtgg tgccgtaaaa tgccgcgctc tgatgcggat
     1201 tctcgccgta gcgcatgtct tgaagtttgc tgacttgtat attgaccgtc tggccccagc
     1261 gttgcggctt tttgtcttgg tcaagtgaag aaaagtagtt ggagatcgcg ccgtcgtagt
     1321 gcgacgtgtg ctgaaacgcc ttacagaaaa gccggaaatg tgtcgcgggt aaaagagcgc
     1381 cgtgattagc ccgcatttcg gcgacgatcg aatcgtaatc cgccggatcg acgaccacgc
     1441 cgacatgctg atgatttttc gccgcggcgc gcaccatgct tgggccgccg atatcgatgt
     1501 tttcgacgat ctcctcgaag gcggcgcctc gggccaccgt ggcctcgaag gggtagagat
     1561 tcacgacgac tagatcgatg ggcttgatgc catgttcttt cattttggcg acatgttcgg
     1621 ggttgtcgcg cagcgccagg atgccgccgt gtatctttgg atggagagtt ttgacccggc
     1681 catccatcat ttccggaaag ccggtgagct cggcaacgtc tttaaccgga atattgctgt
     1741 cgcgcagcag cgccgcggtg ccgccggtgg aaaccagctc gacgcccatg gctgcgaggg
     1801 cacgggaaaa ttcgacaatc ccttgcttgt cggaaacgct gatgagggct gtttcaatct
     1861 tgcccactgc cggtgatcct cctaaacgtt ggtgtgaatg cgcggaacgc gagcgccgat
     1921 ggaggtgaaa atctcgtaag atatggtgtt ggcccaaccg gcgatctcgt cggcgccgat
     1981 ttccgcgccg ccttgagcgc cgagaagaac aacctcgtct ccctgatgga tatttctaat
     2041 atcggtaaca tcgattgtag tcaaatccat gctcactctg ccgaccaccg gcgcgcgttt
     2101 gccgcgtact aaaacttgcg catgattgga aaaaagccgt cgataaccat cggcatagcc
     2161 cactggcaac gtggcgatga ggctgtttcg ctttgtcaca aacgttcggc cgtagccgat
     2221 gctggtgccg gccgggacgc gcttcaactg aatgatgcgc gtctgccagc tcagcacggg
     2281 cttgagttcg atctgcttct tgagtttcag cgccggatac atgccgtaga gaatcagccc
     2341 tgggcggacc atggaaaagt gcgcggtggg ttcggtaata agcgccgcgc tcttggccat
     2401 gtggctggct cgatgcgtga tcttcgctgc tgccaatcgg tctaatactt ggcggaaact
     2461 cttgagttgt tttcgagagt atcgctggtt ggcgctctcg gcatcggaga agtgggaaaa
     2521 taccccgtgc agttttaccg cttcgagttt ggcgagcacc ggcagccaag cgtcggttgc
     2581 atcggtcggg aaaccgatgc ggcccatgcc ggtgtccatt tcgatttcca ctggcaaggt
     2641 cgttttgagg cgttgcagcc gtttgtccaa cagcttcaat gtcacgactt catgaatgac
     2701 cggggttagc cgatttttta aaaactgctc caactgttcg aggtaaacac cagcgagcac
     2761 caggattggc tgcatgatgc gcgcgcgcct gagctcgatg ccttcttcca ccgtggcaac
     2821 gccgaaggcg tcgctgccgg cgccggccaa ggtctttgcc acggcaactg cgccatggcc
     2881 gtatccgtcg gctttgacca tggacaggat tttgacccgt gcgccgacca ggccgcggat
     2941 ctgtttgaag ttccaacgca aggctcgatg gtcgatgacg cagagagtag gccgccctgt
     3001 cggggtggtg atcatgaagg cgaagattgt ccgggcgaat ttattacttg acggtggaac
     3061 cggcgagggc cggtttttcc ggggtcatga agatcacctg ctcgccactg gaggcggcga
     3121 gcaacatacc ttggctttcc aagccgcgca gtttggcggt ggccaggttg gcgacgacga
     3181 cgatttgctt gccgacaagt tgttccggtg tgtagtggtt tctgatgccg gcgcagatct
     3241 gccgctgctc ggtgccgaga tcgacatgca gcaccagcaa tttatcggca ttgggatggg
     3301 gctcagcggc ggtgatcgtc gcgatcttga gctcgatctt tttaaaatcg tcgatggaga
     3361 ttttttccgg ggtggcttca ggttccatgc cgtagaaata tcagaagtgg cgcggcaaaa
     3421 aaagctgcgc tcgtggggcg cagaaatccc atgtgtgctg gctatcacga tcattggcgg
     3481 gctaaccgcc agagcgaggt cacttccgct gcccgcgcga agtgcagggg atcgccggca
     3541 tccgacgcct tgggatgacg tggcttggca tcgattcgac ccatgacctt caaaccggcg
     3601 ttcgcaaacg cggctaatag atcaactcgt gagcgtaagc ccaagtgctc ggcaaagtca
     3661 gataggatga gccagccttc accgctcggc acgagatgag ccgccaagcc attcaggaag
     3721 ccaagcagca tgcggctgtc ctcgtcataa accgcagagt caatgggaga ggcgggccgg
     3781 gcgggcagcc aaggggggtt gcacaccacc aacgacgctc gaccttcggg gaaaagatcc
     3841 gcgcgcatga cctgcacttg ctgggtcagg cgcagccgat cgagattgtc
//
LOCUS       DASQKS010000133         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_73744, whole genome shotgun sequence.
ACCESSION   DASQKS010000133 DASQKS010000000
VERSION     DASQKS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73744"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VE170_03440"
     CDS             complement(<1..978)
                     /locus_tag="VE170_03440"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ14515.1"
                     /translation="MLSHEENDLLTRVGPGTAMGRLMRCYWIPALLSEEIPEPDCPPV
                     RVRLLGEDLVAFRDSSGRVGLLGEHCAHRGTSLFFGRNEDCGLRCIYHGWKYDVAGKV
                     VETPAEPSGSDFRNKLQHTAYPCQEIAGMIFTYMGAPENLPLMPRYEWANLPPRQLFP
                     VKSFLECNYLQGVEGDFDSSHTSFLHNNNLQNGERLKRDGAPTLAAEETGYGMRAVSI
                     RKFGAEQIYVRTSPYIMPSFSIVPGPPTAKFEEDDIRGFRFWVPIDDRSSWLYILNMR
                     QKPFDDTEQKSLRSWVENDYRRKRNLHNHYLIDRQLQRSHLYSGIQAVNP"
     gene            complement(1076..2080)
                     /locus_tag="VE170_03445"
     CDS             complement(1076..2080)
                     /locus_tag="VE170_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14516.1"
                     /translation="MIIDVHGHVSAPTELWAYKANILSARGSHGRGGVNVSDDEIRAA
                     ANRKENWAKGHLDYLKDHGTDLQLISPRPFQLMQSEKPAKLVHWFAEECNNIIARQMK
                     LFPGTFIGVCALPQAAGEPIQNVLGELERCVKLGFVGCLLNPDPFENGGQEAPALGDR
                     YWYPLYEKLCELDVPAHLHGTGSRSERSPYSVHFINEETIAVFGLVNSNVLKDFPKLK
                     IIVSHGGGAIPYQLGRFEAPSLRRDTGARFSDGLRQLYYDTVLYTEDALRLLIKTVGA
                     DRCLFGTECPGTGSSVDPATGRSMDNVKPMLENIETVSATDKQKIFCDNAKAVFNLKI
                     "
     gene            2243..2572
                     /locus_tag="VE170_03450"
     CDS             2243..2572
                     /locus_tag="VE170_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ14517.1"
                     /translation="MWYLVMSRSLPDREEEKERNYEDHRQWLDDQHKAGRLMFSGPTT
                     DGRYGVYVMLAANLEEAKSLAATDPHHQRGIREMEVLEWRAHRAFRLDKPTIADVEAM
                     ARGQPLS"
BASE COUNT          577 a          743 c          734 g          546 t
ORIGIN      
        1 agggttaacc gcttggatac cggagtaaag gtgactgcgc tggagctggc ggtcgatcaa
       61 gtagtggtta tgcaagttgc gcttgcgccg atagtcgttt tcaacccaac tgcgcaagct
      121 tttttgctcg gtgtcgtcga atggcttttg acgcatattc aaaatatata gccaactaga
      181 gcgatcatcg atcggcaccc agaaacgaaa gccgcgaatg tcatcctcct cgaacttggc
      241 cgtcggcggg ccgggcacga tgctgaacga cggcatgata tacgggctcg tgcgcacgta
      301 gatttgctcg gcgccgaatt tacggatcga cacggcacgc atgccgtagc cggtttcttc
      361 cgccgccagc gtcggcgcgc cgtcgcgttt caagcgttcg ccgttttgta gattattgtt
      421 atgcaggaat gacgtgtgcg atgaatcgaa gtcgccctcg acgccctgga gatagttgca
      481 ttcgagaaac gacttcactg gaaacaactg ccgcggcggt aaatttgccc actcgtagcg
      541 cggcatcagc ggcagattct ccggcgcgcc catgtaggta aaaatcatcc cggcaatttc
      601 ctgacaggga taggcggtgt gctgtagctt attacgaaag tcgctaccag acggctccgc
      661 cggggtctcc acgacttttc cggccacgtc gtatttccaa ccatgataaa tgcagcgcag
      721 gccgcaatct tcgttgcggc caaagaacaa cgacgtgccc cgatgggcgc aatgctcgcc
      781 aagcaagccg accctgccgg aggaatcgcg aaacgccacc aaatcttcgc ccaggaggcg
      841 cacccgcacc ggtggacaat cgggctccgg aatttcctcc gacaacaacg ccggaatcca
      901 gtaacaacgc atcaaccgac ccatcgccgt ccccggcccg acccgggtta gcagatcatt
      961 ttcttcatgg gacaacataa gttttcattg gctcgctgat ttgaccgctt cttcagccca
     1021 cttgaacgtt ttaaaacgtt ttgaacggct ttaacatatt gaactcttcg cgcttctata
     1081 tcttcaaatt gaacaccgcc ttggcgttgt cgcagaatat cttttgctta tcggtcgcac
     1141 tcacagtttc gatattctca agcatcggtt taacattgtc catcgatcgg ccggtggccg
     1201 gatcgacgga tgaaccggtg ccgggacact cggtgccgaa caggcaacgg tcggcgccga
     1261 cggttttgat cagcagccgc aaggcgtctt cggtgtaaag cacggtgtca taatacagct
     1321 ggcgcaggcc gtcggaaaag cgcgcgccgg tgtcccggcg cagcgacggc gcttcgaagc
     1381 ggccgagctg atagggaatc gccccgccgc cgtggctgac gatgattttc aatttgggaa
     1441 aatccttgag gacattggag ttgaccaagc cgaacacggc gatggtctct tcgttaataa
     1501 aatggaccga gtaagggctc cgctccgagc gcgacccggt gccatgcaga tgggccggaa
     1561 cgtccaactc gcaaagcttt tcgtacagcg gataccagta gcgatcgccc aacgccggcg
     1621 cctcttggcc gccgttctcg aaagggtccg ggttgagtaa acaaccgaca aatcccagct
     1681 taacgcagcg ctcaagctca cccaagacat tttgaattgg ctcgcccgcc gcctggggca
     1741 aagcgcaaac accgataaac gtacctggga acagtttcat ctgccgggcg atgatgttgt
     1801 tgcactcctc ggcgaaccag tgcaccagtt tcgccggctt ctccgattgc atcaactgga
     1861 atggccgcgg cgagatcaat tgcagatcgg tgccatggtc ttttagatag tcaaggtgac
     1921 ctttagccca gttctccttg cgattggccg ccgcgcggat ctcgtcgtcg gaaacattga
     1981 cgccgccgcg gccatgggag ccgcgggccg acaaaatatt cgccttgtag gcccacaact
     2041 cggtgggcgc cgatacatgt ccatggacgt cgataatcat tgcggtgcct cctcgatttt
     2101 ggcttcgtta gtgggcgtgc cgaagcacgc aacgtcgatg cgaggacatt tgtatccgca
     2161 gcaaattcgc ggtgcaagtg gcaattcggc cgagccatgg ctaagatgca ggtgattggc
     2221 tgtcaacaac aggaggatga ccatgtggta tcttgtcatg tcgcgctcgt tgccggaccg
     2281 ggaagaagaa aaggaacgca attatgaaga ccatcgccag tggctcgacg accaacataa
     2341 ggctggtcgg ctgatgtttt ccggcccaac caccgatggg cgctacggtg tctatgtcat
     2401 gctggcggcg aatctagaag aggcaaaatc cctggccgca accgacccgc accaccagcg
     2461 cggcatccgc gagatggaag tcttggaatg gcgcgctcac cgggcttttc gcttggacaa
     2521 accgactatt gccgatgtcg aagccatggc gcgggggcaa ccgctgagct gagcaatccg
     2581 gacgctgacg cgaccaaaag
//
LOCUS       DASQKS010000134         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3013553, whole genome shotgun sequence.
ACCESSION   DASQKS010000134 DASQKS010000000
VERSION     DASQKS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013553"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VE170_03455"
     CDS             <1..418
                     /locus_tag="VE170_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14518.1"
                     /translation="YAEARQSLERALSLDPNDQLARLYLGLTLARTDDRARGIKEIRS
                     AMRGLYDWLEFINRSRPFEAYWDPLREIRKAIEIHLDSGPGNDAVNSEQLIPDAEWLG
                     NKMEDEIERVRQDERRQYERNFDGRRGSSFGLGIGF"
     gene            complement(506..1267)
                     /locus_tag="VE170_03460"
     CDS             complement(506..1267)
                     /locus_tag="VE170_03460"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14519.1"
                     /translation="MADYISKLLPRFRYGVIQPRANEEVSRARSYQLYKLLPLDFIEI
                     ATGLGLENYTEEGVEKAIKNYWTCVDRLVKEKVDHIIFSGAPISAVLTRSRVLDLLKQ
                     TKERTGITADAPLEALIAALKHLGLTNIAVGSRWADKVNDAIIQYLKEGGIQVLGTTK
                     RNQWASDSFAMSLEEGLAMALEVGREAARTWPEAQAISIPGGAAMALHVIPAIEDEFG
                     KPAFTNMSTEVWNDLVRPGIIPPVQGWGKLLAGGK"
     gene            complement(1339..2322)
                     /locus_tag="VE170_03465"
     CDS             complement(1339..2322)
                     /locus_tag="VE170_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14520.1"
                     /translation="MNIDFHAHIYPEDYLKKLEASSGDVRIESDAKGQKWILSMGARA
                     GPITADFFDTDVRVERIKEHRVDMQILSSPHPGVDRFSPDESAEMSRIINDGIAKAVR
                     KYPKQFQGLAMLPLIDSKLALKELDRAVLDLGLKGMCMLTNVAGKMVDSDFLLPVYAR
                     AQELGVPIFIHPTTPLGAQVMQEWRLAIILGFGFDIVLSATRLAYAGVLDKFPELNFV
                     ISHLGAGIPFLAGRIDRGYHDPTCGIKTKKPTSEYLRDFYCDTVSFYQPALMMAYQFY
                     GAGRMVLGSDFPLIIGDLPAAVPSIEQMPIAQREKQKILGDNVMQLLKLKI"
     gene            complement(2439..3890)
                     /gene="hpaB"
                     /locus_tag="VE170_03470"
     CDS             complement(2439..3890)
                     /gene="hpaB"
                     /locus_tag="VE170_03470"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HYJ14521.1"
                     /translation="MAARTGKEYLAGLADSREIWFDGRRVDGVTAHPLLGRMARTLAE
                     IYDLQYDPALRSQLTYPSPSTGKPVSLSFIQPRSIDDLIRRREMFKHWADFSGGMLGR
                     TPDYLNAILAGCASAKSYFAQNGAVYGERIAAYYELCRERDLCATHTFVDPQINRARS
                     QSEQRDPSVPLHIVGESSDGLIVSGARMLATLAPYADELYVFPSPSRTQQSDAAKFAF
                     AFAVPVATPGLKFLCRESLDFGGAVADYPLTARFEEMDAVAVFHEVVVPWHRVFLKGD
                     IGLCNRLFRETPAFLHGIHQFTVKNLAKAEFILGVASLVAEAIGRTELPAYQQMLGEI
                     VDAVETERAFLRAAEADAITDENGFVHPNPNTLSTARNYFPKIYPRLIEILQLIGSSG
                     LMATPTSHDINSSELGDEIDRYYQSATLMGKERVQLFRLAWDLACSSFAGRQTLYERF
                     FAGDPFLLMASRYTAYDRSAAVARVKAMLARTR"
     gene            complement(4215..>4457)
                     /locus_tag="VE170_03475"
     CDS             complement(4215..>4457)
                     /locus_tag="VE170_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14522.1"
                     /translation="HVVQGQGQTIIDGQSFAWRKGDIIALPPWSVHEHINGSAKADAV
                     LFSIQDAPVLNALGLYYEEEVTDNGGHQKVTSAFGA"
BASE COUNT          891 a         1244 c         1378 g          944 t
ORIGIN      
        1 ttatgccgaa gcgcgccagt cattggagcg cgcactcagt ttggatccga acgatcaact
       61 ggcccggctt tatctcggtc ttacgctggc gcggaccgat gatcgtgcgc ggggaatcaa
      121 ggagattcgc agcgccatgc gcgggcttta tgattggctc gagtttatca atcgcagccg
      181 gccctttgag gcctattggg atccgctgcg ggaaattcgc aaagcgatcg agattcatct
      241 ggacagcggc ccgggcaacg atgcggttaa ttcggagcag ctcatcccgg acgccgagtg
      301 gctcggtaac aagatggaag acgagattga gcgcgtgcgc caggacgagc gccgccaata
      361 cgagcgcaac ttcgacgggc gccggggctc ctcgtttggt ttggggatcg ggttctaaat
      421 cgactccggt gggttgacct taagggtttt tcattcgctc ccaacgtacc agatgttcca
      481 atcgatccgg cacacgctcg cgctgttatt ttccacccgc gagtaatttc ccccaacctt
      541 gcaccggggg gatgatgccg gggcggacta aatcgttcca gacctccgtg ctcatgttgg
      601 tgaacgccgg tttgccgaat tcgtcctcga tggcggggat gacgtggagc gccatggcgg
      661 caccgccggg gattgagatg gcttgggcct cgggccaagt tctcgcggct tcacgtccga
      721 cttcgagggc cattgccaaa ccttcttcca ggctcatggc gaaggaatcc gatgcccact
      781 gatttctttt ggtggtgccg aggacttgga tgccgccttc tttcaagtac tgaatgatcg
      841 cgtcgttgac cttatccgcc cagcggctgc cgacggcgat gttcgtgagt ccgagatgct
      901 tgagcgccgc gattagcgct tccaacggcg cgtcggccgt gatgccggtc ctttctttgg
      961 tttgcttgag taaatccagc accctactcc gggtcaacac cgcggagatg ggcgcgccgc
     1021 tgaaaataat atgatcgact ttttctttga ccaggcggtc gacgcaggtc cagtaatttt
     1081 tgatcgcttt ctcgacccct tcttcggtgt agttctccag acccaggccg gtggcgattt
     1141 caatgaagtc gagcggcagc agtttataca actggtaact gcgcgcgcgc gaaacctcct
     1201 cattggcccg cggttggatg acgccgtagc ggaaccgggg tagtaatttg ctgatgtagt
     1261 cggccataaa tcacctcctg atatttgtcg tcaattgtta agaagagcga ccggcggccg
     1321 ctccggctgt cggcaaattt aaattttcag tttcaaaagt tgcatcacgt tgtcgccgag
     1381 gattttttgt ttttcccgtt gcgcgatcgg catctgctcg atgctcggca cggcggcggg
     1441 caggtcgccg atgatcagcg gaaaatcgct gccaagcacc atgcgtccgg cgccgtaaaa
     1501 ttgataggcc atcatcagag cgggctggta aaacgacacg gtgtcgcagt agaaatcgcg
     1561 caggtattcc gaggtcggtt tcttggtttt gatgccgcaa gtgggatcgt gatagccgcg
     1621 gtcgattctg ccggcgagaa atgggatgcc ggcgccgaga tgggagatca cgaagttgag
     1681 ttcggggaat ttgtcgagca ccccggcgta ggcgagccgg gtcgccgaca atacgatgtc
     1741 gaacccaaag ccgaggatga tcgccaggcg ccactcctgc atgacttgtg cgcccaacgg
     1801 cgtggtcggg tgaatgaaga tcggcacgcc aagctcttgc gcgcgcgcgt agaccggcag
     1861 gagaaaatcc gagtcgacca tcttgccggc gacgttggtg agcatgcaca tgcccttcaa
     1921 gccgagatcg aggacggcgc gatcgagttc ctttagcgcc aatttgctat cgatcaacgg
     1981 caacatggcg aggccctgga actgcttggg atattttctt acggctttgg cgatgccgtc
     2041 gttgattatc cggctcatct cggcgctttc gtccggtgag aaacggtcga cgcccggatg
     2101 cggcgacgat aagatctgca tatcgacccg gtgctctttg atgcgctcga ctcgcacgtc
     2161 ggtatcgaaa aaatcggcag tgatcggccc ggcccgggcg cccatgctca agatccattt
     2221 ctggccttta gcgtcgctct cgatgcgcac gtcgccgctg gaggcttcga gtttttttaa
     2281 ataatcttcc gggtatatgt gggcgtggaa gtcgatgttc atgattcgct cctgaatttg
     2341 ttcaaattgt tcaaaacgtt ctagccgttc aaatcattgg cgcccgtctc agattccttc
     2401 cccattccgt ggaaagtaag gatggccgca tgcaacaatc accttgtccg cgccagcatc
     2461 gctttgaccc gcgccacggc ggcgctgcgg tcgtaagcgg tgtaacggct cgccatcaac
     2521 aagaaagggt cgccggcgaa gaagcgctcg tacagggttt gccggccggc gaagctgctg
     2581 cacgctaggt cccaggcgag gcgaaaaagt tgcacccgct ctttgcccat cagcgtcgcc
     2641 gactgatagt agcggtcgat ttcatcgccg agctcgctcg agttgatatc gtggcttgtc
     2701 ggcgtcgcca tcaggccgct ggagccgatc agctgcaaga tttcgatcaa acgcgggtag
     2761 attttcggaa aataattgcg cgccgtcgat agagtgttcg gattcggatg gacaaagccg
     2821 ttttcgtcgg tgatcgcgtc ggcctcggcg gcgcgcaaga aggcgcgctc ggtttcgacg
     2881 gcgtcgacga tctctccgag catttgctgg taggccggca attccgttcg gccaatcgct
     2941 tcggcgacca gactggcgac gccgaggata aactccgcct tggcgagatt tttcacggtg
     3001 aactgatgga tgccgtggag aaacgccggg gtctcgcgga agagccggtt gcacaggccg
     3061 atatcgcctt tcaaaaacac ccgatgccat ggcacgacga cttcgtgaaa gaccgccacc
     3121 gcgtccatct cctcgaagcg cgcggtgagc gggtagtcgg cgacagcgcc gccgaagtcg
     3181 aggctctcgc ggcagaggaa tttcaaaccc ggtgtcgcca ccggcacggc gaaggcgaag
     3241 gcaaacttcg ccgcgtcgct ttgttgggtg cgcgacggcg acgggaagac atagagttca
     3301 tcggcatagg gcgccagggt ggcgagcatg cgcgcgccgc tgacgatcag tccgtcgcta
     3361 ctttcgccga cgatatgcag cggcacgctg ggatcgcgct gttctgactg gctgcgcgcg
     3421 cgattgattt gcgggtcgac gaaagtatgc gtcgcgcaca gatctctttc gcggcaaagt
     3481 tcgtaatagg ccgcaatgcg ctcgccgtag acggcgccgt tttgcgcgaa ataggatttc
     3541 gccgaagcgc aaccggcgag aatcgcgttc aaataatccg gcgtgcggcc aagcatgccg
     3601 ccgctgaaat cagcccaatg cttgaacatc tcgcggcgcc gaatcaaatc atcgatgctg
     3661 cgcggttgaa tgaaagacag gcttaccggc ttgccggtgg atggcgaagg ataggtcagc
     3721 tgcgagcgta gtgcaggatc atactgcaag tcgtaaatct cggcgagggt gcgcgccatg
     3781 cgccccaaga gcggatgggc ggtgacgcca tcgacacgtc tgccatcaaa ccagatctcg
     3841 cgcgagtcgg cgaggccggc gagatactct ttacccgtcc gtgcggccat tcgatgagct
     3901 ttcttatgat tgtcttgaac gtgggcggac agtagccgag gcggcggcgc gctgtcaaca
     3961 attgcggcgt gctaaggtca cagtgtagtg gaagctctca taacgaaaac gccctatcgc
     4021 acacccaggg cacggaggac gagcgcggcg aagccgcaac cgaaaggaca atggccgcaa
     4081 agaacgcaaa agtcgcaaaa tagaatcggg aggtgtaggc aatgctttga cgcgacctaa
     4141 cggcttcggc gcatcgataa attgcccaag ccgcacaacc taacgggcca cgggcgtcgc
     4201 gccgcgacga cggctcaagc gccgaatgct gaagtgactt tttggtggcc gccgttatcg
     4261 gtgacctctt cttcatagta gaggccgaga gcgttgagca cgggagcgtc ttggatcgag
     4321 aacagcaccg cgtcggcctt tgccgagcca ttgatatgtt cgtgcaccga ccagggcggc
     4381 agggcgatga tgtcgccttt gcgccaggcg aagctctggc cgtcgatgat cgtctgcccc
     4441 tggccctgga cgacatg
//
LOCUS       DASQKS010000135         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1755969, whole genome shotgun sequence.
ACCESSION   DASQKS010000135 DASQKS010000000
VERSION     DASQKS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755969"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="lpxB"
                     /locus_tag="VE170_03480"
     CDS             complement(<1..955)
                     /gene="lpxB"
                     /locus_tag="VE170_03480"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HYJ14523.1"
                     /translation="MLVVGEASGDVHGANLVQALHRQDSQLKIFGVAGEQLQKTAFET
                     LFNVSKLTGMGLVELAGNVKNIWRAYHLLRDALKQRRPDLLILIDFPDFNLRLARIAK
                     SLRIPVLYYISPQIWAWRRGRVRKIAKTVDRMAVVFPFEVDFYRRHGVNVEFVGHPLL
                     ETVTTNQSRDTVLAHLGLDPTKKTVALLPGSRDGEVSRHLPVMLAAALQMRTNDNVQF
                     FCLRASTIDAGELIAMIGDSKIPVVERDRYDAVGAADLVWTASGTATLETALLTRPMV
                     IIYRVSWLTYWIARLLVRVDHIGMVNLIAGKRLVPELLQGEV"
     gene            complement(972..1931)
                     /locus_tag="VE170_03485"
     CDS             complement(972..1931)
                     /locus_tag="VE170_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ14524.1"
                     /translation="MSSEILPVAVVGVGYLGKFHAEKYASSEKANLIGVVDVDGDRAR
                     EIGQLVGAPAFTDYHELFGRIRCASIAVPTRYHHQVARDFLDAGIDVLVEKPLTADLT
                     EANELVALAAAKGALLQVGHLERFNPAIRRLEGIIKDPKFVECHRLAPFIERGTDVDV
                     VLDLMIHDIDVITSLVRSPILRVESVGVPVLTEMPDIANARITFENGCIANVTASRVS
                     IKRERKVRFFQPDAYISIDYDQRRAQIYYKPKPGAGWLDIRAENIEIKDGDALADEID
                     SFLDCVRTRTPPLVGGTEGLRALEIAWTISAQLANLSNQPARG"
     gene            complement(1943..2767)
                     /gene="lpxI"
                     /locus_tag="VE170_03490"
     CDS             complement(1943..2767)
                     /gene="lpxI"
                     /locus_tag="VE170_03490"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707637.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HYJ14525.1"
                     /translation="MNNIGNIGKIGLIAGNGKFPMIFAEEAKRQGYSVVAVAHREETD
                     AALEKHVDEFTWIYVGQLGKIIRTFQKAGVTEAVMAGGIRKVRLLGNFRPDLRGARFL
                     TRIKSRDDDALLRGIADELASDGITIVDSTFCLSSIIPQEGVLTRRAPNAAQWQDVAM
                     GLRLAKEIGRLGIGQMVVVKNQVIVAVEAVEGTDAAIQRGGRLAKSGCVVVKVSKPQQ
                     DMRFDVPAVGVETINALSQVGGAVLAIEAGKAILIEKDELLRVADAAGIAVVAVAA"
BASE COUNT          661 a          939 c          848 g          630 t
ORIGIN      
        1 tgacctcgcc ttggagcagc tccggcacca ggcgcttccc ggcaatcaaa ttgaccatgc
       61 cgatatggtc cacccggacc aggagccggg caatccaata agtgagccac gacactctat
      121 agatgattac catgggccgg gtcaggagcg cggtttcgag agtcgccgtg cccgaagccg
      181 tccaaaccag gtcggccgcc ccgaccgcgt cgtagcgatc ccgttcgacc acgggaattt
      241 tgctatcgcc aatcatcgct atcaactcgc cggcatcgat ggtgctcgca cgcagacaaa
      301 agaattgcac attatcattg gtgcgcatct gcagcgcggc agccagcatc accggcaggt
      361 gacggctgac ctcgccatcg cggctgcccg gcaacagggc gacggttttt ttagtgggat
      421 cgaggccgag atgcgccagt accgtgtcac gactttgatt cgtggtgacc gtctctagca
      481 atggatggcc gacaaactcc acgttcacgc catggcgacg gtaaaaatca acttcaaagg
      541 gaaacaccac ggccatccgg tcgaccgttt tggcgatctt gcgcacccgc ccccggcgcc
      601 aagcccaaat ttgcggactg atgtaataga gcacgggaat tcgcagagat ttcgctattc
      661 gcgccaggcg cagattgaag tcgggaaaat cgatgagaat cagcagatcg ggccggcgct
      721 gtttgagcgc atctctcaac aggtgatagg cgcgccaaat attttttacg ttgccagcga
      781 gctccaccag ccccatgccg gttagtttcg atacgttaaa aagcgtctcg aatgcggttt
      841 tctgcaactg ctcgccggcg acaccaaaga tcttaagctg ggaatcctgt cgatgcaggg
      901 cttgcaccag gttggcgcca tgaacatcgc cggatgcttc gccaaccacc aacatgacct
      961 gcctcgtttg cttagccacg cgcaggttga ttcgaaagat tggccagctg agcgctgatc
     1021 gtccaggcga tttccaatgc ccgtaagcct tccgtgccac cgaccaaggg cggcgttcgc
     1081 gtgcgcacgc aatcgaggaa tgaatcgatc tcatcggcca gagcatcgcc atctttgatc
     1141 tcgatattct cagcgcggat atcgagccaa ccagcgcccg gcttgggctt ataataaatc
     1201 tgcgcgcgcc gttgatcgta gtcgatggat atataggcgt ccggttggaa aaaacgcacc
     1261 ttgcgttcgc gcttaatcga cacccggctg gcggtgacat tggcgatgca accgttctcg
     1321 aaggtaatac gcgcattggc aatgtcaggc atttcggtca acaccggcac gcccaccgac
     1381 tcgacccgca ggatcggcga gcgcaccaag ctggtgatca catcgatatc gtgaatcatc
     1441 agatccaaga ccacatcgac atccgtgccg cgctcgataa acggcgccaa gcggtggcat
     1501 tcgacgaact ttggatcctt gatgatgcct tcgagtcggc gaatcgccgg attgaaccgt
     1561 tccagatggc cgacttgcaa taacgcgccc ttggccgccg ccagcgcgac tagctcgtta
     1621 gcctcggtca aatcggcggt caaaggtttt tcaactagaa catcgatgcc ggcgtcgaga
     1681 aaatcgcggg cgacttgatg atgatagcgc gtcggcacgg cgatactggc gcagcgaatc
     1741 cggccaaaca gctcatgata gtcggtaaac gccggtgcgc cgaccaattg gccgatctcc
     1801 cgggcgcggt cgccgtcgac gtcgaccacg ccgatgaggt tagccttttc cgacgacgcg
     1861 tacttttcgg cgtggaactt gccgagatat ccgacgccga ccactgctac cggtaaaatt
     1921 tccgacgaca tattcagcgt tactaagcgg cgacagcgac gacggcgatg ccggcggcgt
     1981 ctgctacacg cagcagttca tccttttcga tcaatatcgc cttacccgcc tcgatggcaa
     2041 gcacggcgcc gccgacctga ctcagcgcat tgatcgtttc gacaccgacg gcgggaacgt
     2101 cgaagcgcat gtcctgctgc ggcttactga ccttgaccac gacacagccg gacttggcca
     2161 atcggccgcc ccgttgaatg gctgcgtcgg tgccctccac agcctccacg gcaacgatca
     2221 cttgattctt gaccactacc atttggccga tacccaagcg gccgatttcc ttcgccaaac
     2281 gcaggcccat ggcaacgtct tgccattgcg cggcattggg cgcgcggcgg gtcaggaccc
     2341 cttcttgtgg aatgatgctc gacaaacaga aagtcgaatc gacaatcgtt atgccgtcgc
     2401 tggccagttc atccgcgata ccgcgcagca gcgcgtcgtc gtcgcggctt ttaatcctgg
     2461 tgagaaaccg cgccccgcgc agatccgggc gaaagttgcc aagcagcctg accttgcgaa
     2521 taccgccggc cattaccgcc tcggtcacac cggctttttg aaacgtcctg atgatcttgc
     2581 ccagctggcc gacatagatc caggtaaatt cgtcgacgtg tttttccagc gctgcatcgg
     2641 tctcctcgcg atgcgccacg gccaccaccg agtagccctg acgtttagcc tcctcggcaa
     2701 agatcattgg gaatttaccg ttgccggcga tcaatcctat cttgcctata ttgccgatat
     2761 tgttcatcgc tgtggatcag tcagcggcgg cggcgcggtt ggcaaagcca gtctgaatta
     2821 tgcatgctgc cggaaacgac agagtggtta actaggtgga accctagctg aaaagtcgag
     2881 cgaaaacatc cgcgccaaaa gtttcacgtc atgggccggc gcttggcaag cgttttgact
     2941 ttcacaggtg gtttcacagc agatgaccgc gcttgcgtca tcaatgcaga gctgctctgc
     3001 cttcgctctg gaagggtttc gcctcgatcg ccgatcgcca tcacctggcg ggtgagcaat
     3061 ctaacggcac acgccgcg
//
LOCUS       DASQKS010000136         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3017304, whole genome shotgun sequence.
ACCESSION   DASQKS010000136 DASQKS010000000
VERSION     DASQKS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017304"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1119
                     /locus_tag="VE170_03495"
     CDS             244..1119
                     /locus_tag="VE170_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ14526.1"
                     /translation="MIGSIQVFLVCLTIVNAAGAFAQERFSFFQASTPESVERMLKLA
                     NLRDDDVIVDLGSGDGLIVLTAAKLNTKLRGWGVDIDQKLIAESNGVAKAQGVADRIK
                     FFHQNAFDADLREATVITLWLFPELMRLLRPIIFERARPGTRVLTSTWDFGTWPADTI
                     DAGNPSIHLWTVPARVAGNWSWELSLANQHVAYAAVLDQQFQAAEGVVRAGANREVLT
                     DVKLTGENISFNLAMTLEGLGLTRHEFTGKIRGEQIDGTAQLILPEGNRLELPWRASR
                     TPASDYFAPTGANIP"
     gene            complement(1215..1496)
                     /locus_tag="VE170_03500"
     CDS             complement(1215..1496)
                     /locus_tag="VE170_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14527.1"
                     /translation="MAFVRGTLKALQYSLKNRPEMIKIMPWYLGIKDASLIDQLYDLY
                     LTRQSVDGSVDENWMNGAIEFTQKTLGGPAKEVLPSQVFDFSFGQNSLR"
     gene            1682..2950
                     /locus_tag="VE170_03505"
     CDS             1682..2950
                     /locus_tag="VE170_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14528.1"
                     /translation="MAKKGFKVFDSDMHIMEPPDLWERYIDPKFRDQAPRGRTSENVR
                     DLGTIFPNVPKDTRRTGGTPHRGSNFNKNQEIYRSHAERGWTGHVQLEAMDTEGIDTA
                     VLFPTRGLGILTFPDQDPRFADAMARAYNDWLHDFCSADPNRLLGAGMISVYDLEDAL
                     RETRRVKEEYGFRSVFLRSNIINGKNWYDPHYEPLWDLLEELNLPLGFHEASASRSRQ
                     AAEHFEPNFGLRRVYAQPFEQMMGMGAFLGGGILERHPKLRVAFLEANCSWLPWLLWR
                     LDEGYEREGDVFMPDLKMTPTEYFKRQCWISIEPDESPARYTIEEFGCDQLVFSTDYP
                     HGDSKYPHAVESFLELTLSDDNKRKILWDNCARFYGAGEYEPRAAAAKPQQRKSNHKP
                     GGHHGNPNRKCHANNNEQRKRQGSFHSEDP"
     gene            2868..3506
                     /locus_tag="VE170_03510"
     CDS             2868..3506
                     /locus_tag="VE170_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14529.1"
                     /translation="METQTASATQTTTSSENARDRFIRKTRELFAKEADLDKRWNGLR
                     PILAELLADPDMIAASKKWPDCVPANGRAENLLFYEDPDYGFAINGLTKGQARQGVAA
                     RIHDHAHIYTLYGVIDGREKVVRYDRLDDRSKPNFAQIKESANVLCGPGDIDLVKPYE
                     IHTEVTVGERTVAVIVRSQKGGDFNQGRYNTETNEYFESLGPRQTPCEMMPT"
     gene            3541..4377
                     /locus_tag="VE170_03515"
     CDS             3541..4377
                     /locus_tag="VE170_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HYJ14530.1"
                     /translation="MSRAESVEEIAPAFEALLHYLADTTEAPESYGGISQSEADQKRK
                     GKYAEHSMPIYNGRAVAHGLSLERQGFILVDHNTQVKNFYDEAEVRSVYYKETEELVK
                     RITGAKRVLVFDHTLRAADSAIREKNQVSSPVRNAHNDYTEWSGPQRVRDLLPQEADD
                     LLSRRFAVVQTWRPINKPVQNEHLAIADARSIGTNELVPSARIYPNRRGEVYHSTYNP
                     EHRWYYFPNMQRNEAMVFKCYDSSKDGRARWTAHCAFDDPTAPTNVPPRESIEMRTLA
                     FF"
BASE COUNT         1017 a         1337 c         1183 g          853 t
ORIGIN      
        1 gctgacccca atgactataa cgagcccgat caatatccga agcatcatac tctcagctcc
       61 tacggtggtt ggcccgtgaa ctgcccgaga tgtatttggc cacgcagcac ttgtcaattg
      121 accgttaaga aagttaattg gttcatttcg ataataacga atcgtcaact tcggagcaga
      181 tccctggtca gctccctggg aagtcgacca cggggcacgc gccaatacag caatggagac
      241 aacatgattg gttcaattca ggtattccta gtttgtctca cgattgtaaa cgccgccggc
      301 gcattcgcgc aggagcgctt ttcgtttttt caagcgagta ctcccgagag cgtcgagcgc
      361 atgctcaagc ttgccaactt gcgcgacgac gacgtcatcg tcgacctcgg ctcaggcgac
      421 ggcctcatcg tcctcaccgc cgccaagctg aacacaaaac tgcgcggttg gggcgttgat
      481 atcgaccaga aactgatcgc cgaatccaac ggcgtcgcca aagcccaggg cgtcgccgac
      541 cggataaaat ttttccacca gaatgccttt gacgccgact tgcgcgaagc cacggtgatc
      601 accctgtggt tattcccgga gctgatgcgc ctgctcagac cgattatttt cgaacgagcg
      661 cgcccaggca cacgtgttct aactagcacc tgggacttcg gcacttggcc cgccgacacg
      721 atcgacgctg gcaatccatc gatccatctc tggaccgtgc cggcccgcgt cgccggcaac
      781 tggagctggg aattgtcctt agccaaccag cacgttgcct acgccgcggt gctggaccag
      841 caatttcaag ccgccgaagg cgtggtccgc gccggcgcca accgtgaagt gctcaccgat
      901 gtgaagctga ccggcgagaa catttccttc aatctggcca tgactcttga agggttgggc
      961 ctcacccgcc acgagttcac cggcaaaatc cgcggcgagc aaatcgacgg aaccgcgcag
     1021 ctgatcttac cggaaggaaa tcggctcgaa ctaccctggc gcgcgtcgcg cacaccagcc
     1081 tcagactact ttgcgccaac cggcgcgaat ataccgtgat aagctaaatg atataagttt
     1141 gagccaaatg gctttttcta ttaaggcggg ccgtggcgaa gatacccaac aatcaacctc
     1201 cagtgaccgg caatttaccg caagctgttt tggccgaaac tgaaatcaaa cacctggctc
     1261 ggcagaactt ccttggcagg accgccgaga gttttctgag tgaactcgat agccccattc
     1321 atccaatttt cgtccaccga gccgtccacc gattggcggg ttagatagag atcgtagagc
     1381 tggtcgatga gcgaagcgtc tttgatgccc aaataccacg gcataatttt tatcatctcg
     1441 gggcggtttt tcagcgaata ctggagcgct ttgagggtgc cgcgcacgaa tgccatgacc
     1501 tcggcaggat tggttttgat tttttcgtcg actggccaac atcttaatca cagcgccgat
     1561 tcggtggccc agtgaaatac atcatcatct gcgcaacgta tgcaaattca acgcggcgac
     1621 gcggcccttg cttgacatcc gccctcgctc ggcattacgc tcggaaatcg gaggggaaac
     1681 catggcgaaa aaaggtttta aagttttcga cagcgacatg catatcatgg agccgcccga
     1741 tctttgggag cgctacatcg atcccaagtt tcgcgaccag gcgccgcgcg gcagaacttc
     1801 ggaaaacgtg cgcgatctcg gcacgatatt tcccaacgtc ccgaaagaca cccggcgcac
     1861 cggcggcacg ccacaccgcg gcagtaattt taacaagaac caggaaatct atcgcagcca
     1921 cgccgagcgc ggttggaccg gacatgtgca attggaggcg atggacaccg aaggcatcga
     1981 taccgcggtg ctgttcccga cgcggggctt gggcatcctc acctttcccg accaagatcc
     2041 gcgcttcgcc gacgccatgg cgcgcgccta taacgattgg ctgcatgact tctgctccgc
     2101 cgatcccaat cgcttgctcg gcgcaggcat gatttccgtc tacgatctcg aagacgcttt
     2161 gcgcgaaacc cgccgggtga aggaagagta cggtttccgt tcggtgtttt tgcgttcgaa
     2221 catcatcaac ggtaaaaact ggtatgaccc gcattacgag ccgctgtggg atttgctcga
     2281 agaattgaat ctgccgctgg gctttcacga agcctcggca tcacgctcgc gccaggccgc
     2341 cgaacatttc gagccgaact tcgggctgcg ccgtgtctac gcccagccct tcgagcagat
     2401 gatgggcatg ggcgcctttt tgggcggcgg gattctcgaa cgtcatccca agttgcgtgt
     2461 cgctttcctc gaagccaact gttcctggct gccatggttg ctctggcgtc tggacgaagg
     2521 ctacgagcgc gagggcgatg tcttcatgcc ggatctgaaa atgacaccaa cggagtattt
     2581 caaacgccag tgctggatct ccatcgagcc ggatgagtcg ccggcgcgtt atacgatcga
     2641 ggaattcggc tgcgaccagt tggtgttttc caccgactat ccccatggcg actcgaagta
     2701 tccccatgcc gtcgaaagct ttcttgaact gacactttcc gacgacaaca agcgcaagat
     2761 cctatgggac aactgcgcgc gcttttacgg tgccggagaa tatgaaccgc gcgccgcagc
     2821 cgccaaacca cagcagcgga aatccaacca taaaccagga ggacatcatg gaaacccaaa
     2881 ccgcaagtgc cacgcaaaca acaacgagca gcgaaaacgc cagggatcgt ttcattcgga
     2941 agacccgtga gctattcgcc aaggaagccg atctcgacaa gcgttggaac ggtctgcggc
     3001 caatcttggc cgagctcttg gccgatcccg atatgatcgc cgcgtcgaaa aaatggcccg
     3061 attgtgtgcc cgcgaacggc cgcgccgaga atctcctgtt ctacgaggac cccgattacg
     3121 gcttcgccat caatggcctg accaaaggcc aggcgcgcca gggtgtcgcc gcccggattc
     3181 atgatcacgc ccacatctac acgctctacg gcgtcatcga tggccgagag aaagtcgtgc
     3241 gctacgaccg cctcgacgat cgctcgaaac cgaacttcgc gcagatcaaa gaatcggcca
     3301 acgtgctctg cggccccggc gacatcgatc tggtgaaacc ctacgaaatc cacaccgagg
     3361 ttaccgtcgg cgaacgcaca gtagccgtga tcgtccgcag ccagaaaggc ggggacttca
     3421 accagggccg gtataatact gagaccaacg aatatttcga aagtttaggc ccacggcaga
     3481 ccccctgtga gatgatgccg acgtaaggca aatattttcc gcagagttga ggagtcgctt
     3541 atgtcacgag ccgaatccgt cgaagaaatc gcacccgcct tcgaagcgct gctgcactat
     3601 ctcgccgaca ccaccgaagc cccggaaagc tacggcggta tcagccaatc ggaggcggat
     3661 caaaagcgca agggcaaata cgccgagcat tcgatgccga tctacaacgg ccgcgccgtc
     3721 gcccatgggc tgtccctgga acgccaagga tttatcctgg tcgatcacaa cacccaggtg
     3781 aagaattttt acgacgaggc ggaggtgcgt tcggtctact acaaagaaac cgaggagtta
     3841 gtgaagcgca ttaccggcgc gaaacgggtg ttggttttcg atcacacatt gcgcgccgcc
     3901 gacagcgcca tacgcgagaa aaatcaggtg agcagcccgg tgcgcaacgc ccacaacgac
     3961 tatacggaat ggtccgggcc gcagcgcgtg cgcgacctgc tgccgcagga agcggacgat
     4021 ctcctcagcc gccgcttcgc ggtcgtgcaa acctggcggc cgatcaataa gccggtgcaa
     4081 aacgagcatc tcgccatagc cgacgcccga tctatcggca cgaacgaatt ggtcccgagc
     4141 gcgcgcatct atccgaaccg ccgcggcgag gtttatcact ctacctacaa cccggagcac
     4201 cgctggtatt atttccccaa catgcagcgc aacgaagcaa tggtcttcaa gtgctatgat
     4261 tcatcgaaag acggccgcgc ccgctggacc gcgcactgcg cttttgacga ccccacggcc
     4321 cccaccaacg tgccaccacg cgagagcatc gaaatgcgca ccttagcttt cttctagatc
     4381 accgcggtga
//
LOCUS       DASQKS010000137         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4277080, whole genome shotgun sequence.
ACCESSION   DASQKS010000137 DASQKS010000000
VERSION     DASQKS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277080"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..916
                     /locus_tag="VE170_03520"
     CDS             185..916
                     /locus_tag="VE170_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14531.1"
                     /translation="MFNLKSLVSIILVLAAWCGSSLAQDDRWFFTAKEIADAYRYQQQ
                     FGARLHNPLAPEACWQGQMEFAASSQGRRFAAPCRFISETTRQLRELLESGAAKYLFP
                     LDVAYADLAVPADIHESKYQQLSSAEMLPALLREPALVAIYHTALHLSPESTKGAASV
                     WQEKRTVAGFFDGRPNQLLSRRLDGGDDDAAGLVRVGRFAVLSHFLGELTLVAGETVV
                     TFDLSFDNDRAAAAPDILTVSVTAR"
     gene            1257..1859
                     /locus_tag="VE170_03525"
     CDS             1257..1859
                     /locus_tag="VE170_03525"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14532.1"
                     /translation="MREILTGIFTWPWFSEPHGYNFNGHFIQHAQGNLCVDPVEPSDE
                     DLAELARQGVARILITNRNHSRAANKIRGRTGARTAIHPADAPHARSEGTVLDDELNP
                     SDKVGPFVVIDSPGKSLGEVVLHWPERKILLVGDAVVGAPPGKCKLLPDKVVDDPPRL
                     RDSARQLLALDFDILLVGDGEPILQSAKERLRDLVNTFPQ"
     gene            2041..>2271
                     /locus_tag="VE170_03530"
     CDS             2041..>2271
                     /locus_tag="VE170_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14533.1"
                     /translation="MASTFCRSLRFLAFLLTLGLPSATNAQPLPRSVTVASNPPGTVF
                     YALASGLSKVAGEGATFQMVVQPYSGTSTFLPL"
BASE COUNT          440 a          640 c          657 g          534 t
ORIGIN      
        1 ataagcgctt ttgccaatcg tttgcttgga ctttttcgag cgattgtctg gccaggcaca
       61 gtccttgcgc tggaaacgat gtagaagcac ttgaaagttc tagacgactt ccgctcatgg
      121 cttcggcgcc ggttcgccgg cgccttcggg ctttgatcgg gagcaaacca tcggtgaggt
      181 gtttatgttc aacctcaaat cacttgtgag catcatctta gtcctcgccg cttggtgcgg
      241 atcatccttg gcgcaagacg accggtggtt tttcacggcc aaggagatcg ccgatgccta
      301 tcgctatcag caacagttcg gcgcgcgcct gcataatccc ttggcgcccg aagcctgttg
      361 gcagggccag atggaattcg ccgcgtcgtc ccagggcagg cggtttgcgg cgccttgccg
      421 gttcatcagc gagaccactc gccagttaag ggagctgttg gaatcggggg ctgcgaagta
      481 tctcttcccg ctggatgtcg cttatgccga tttagcggtc ccggcagata tccacgagag
      541 caaataccaa caactatcga gcgctgagat gctgccggcg ctgctccgcg aaccggcgtt
      601 ggttgcgatc taccatacgg cgctgcatct atcgccagag tcaactaaag gagctgcgag
      661 cgtgtggcaa gaaaagcgca ctgtcgccgg cttcttcgat gggcggccga accaactcct
      721 gtctcgacga ctggatggcg gcgacgacga cgcggcaggg ttggttcggg ttggccgctt
      781 tgccgtcttg agccatttcc tgggcgagct aaccctggtc gcaggtgaaa ccgtcgtgac
      841 ctttgatttg tcgttcgaca acgatcgcgc ggcggcggcg ccggacatct tgaccgtgag
      901 cgttaccgca agataatccc cgctccgact ctgttttgga tcaacttttt taagttgtta
      961 gccctaacgg cagacaccgg cgcggcattt gacaaaccca tgcggtttgt tctgctcgtc
     1021 gaaaaatttg tcggacctaa ttatttgcct tagtttccgt ttcacttttg atcaggccat
     1081 caattcagcc cgtcaactag atctattgtc tgagcaagat taatttgtca tctgcgcgcg
     1141 atcgtctaag caatgccata gcggttttcg ctgccgcacg catcgtcttc aaagaaattg
     1201 tgctaggcta aacaataatt cgggcgagaa actgagatta acgtaaggga gttaatatgc
     1261 gcgaaatcct gacggggatt tttacctggc cgtggttttc ggagccgcat ggttacaatt
     1321 ttaacggcca cttcattcag cacgcccagg ggaatctttg cgtcgatccc gtggagccga
     1381 gtgacgaaga tctggcggag ctggcgcgcc agggcgtcgc gcgcattctc atcaccaacc
     1441 gcaaccattc gcgggcggcg aataaaattc gtgggcgcac cggcgcgcgc acggcgatcc
     1501 atccggcgga cgcgccccac gcgcgcagcg agggaacggt gctcgatgac gaattgaatc
     1561 caagcgacaa ggtcggcccg ttcgtggtga tcgactcgcc gggaaaatcc ctcggcgaag
     1621 ttgtccttca ctggcccgaa agaaaaatct tgctggttgg cgatgcggtc gtcggtgcac
     1681 cccccggaaa gtgcaagctc ttaccggaca aggttgtcga cgatccgccg cgtctgcgcg
     1741 atagcgcgcg acagctgctg gcgctcgact tcgatattct cttggttggc gacggcgagc
     1801 caattttaca gtctgcgaaa gagcggctgc gcgacctggt caatactttt ccccagtaat
     1861 aatcgccgcc aacggcgacg atcgacaagt ctccggctac gtgctgcaga taagaatgag
     1921 atcgacgcgg aagtctttcg tactgacacg gttttccctg atgcgccgcg ccgccgtgtt
     1981 ttcgccttga cagcgcgtct tggttctcta tacaagtcgg ccatcgaagg aggaaactct
     2041 atggcttcga ctttctgtcg gtctttgcgc ttccttgcct ttctgctcac gcttggactt
     2101 ccctcagcta caaatgcgca gccattgccg cgctcggtga cggtggcgtc caatccgccg
     2161 gggacggtgt tctatgcgct ggcgagcggg ctctccaagg tcgccggcga gggggcaacg
     2221 tttcagatgg tggtgcagcc ttattcgggc accagtactt ttctgccgtt g
//
LOCUS       DASQKS010000138         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1758435, whole genome shotgun sequence.
ACCESSION   DASQKS010000138 DASQKS010000000
VERSION     DASQKS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758435"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VE170_03535"
     CDS             complement(<1..655)
                     /locus_tag="VE170_03535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14534.1"
                     /translation="MIGRRVSFLLVLFCVAWLAASARAEKIRTAIPGANLNYLSVYSA
                     EERKFFRDEGLENETIAIGGPAGIAALVSGDVDFSGAGGSGLRGAVRGAPLKAIFYQT
                     EKPTWYLVVHPSISQGSDLKGKKIGVALIGDSEDRFITLYAERAGVSAKEITRISLGT
                     SIGDKILGLKSGNVAAVVLDPAGTVAAEREGLRKLVFMGDIFTLPFQGYVATNQKLAE
                     "
     gene            complement(839..2020)
                     /locus_tag="VE170_03540"
     CDS             complement(839..2020)
                     /locus_tag="VE170_03540"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HYJ14535.1"
                     /translation="MPNDPRTHGLHETCNQRGSHRPLFTKGGPPNYIEKSLAAAAEPF
                     KGITADGDVIADLFGLQQTGLSTKVIREAAEAFLATLSADQRARTLFPVDTDQWRKWS
                     NIHPTLLRHGMALFEMSDRQRDCAFALLRESLSQRGFEETLDLMHLNETVLELTGRLD
                     EYGEDLYWLSIMGAPSATEPWGWQWDGHHLIINYFVLGEQIVVTPTFLGAEPVHAVGG
                     KYAGVRAFKGDEDRGLALFRALTQSQRVKTVIAPETSGEDFTTAFRDNLVLDYQGIRA
                     GELSSAQHELLLGLVEYHVGRMRDGHAQVKMDEVKRHLNDTYFCWMGGGADDGVFYYR
                     VQSPVIIVEFDHQRGIAFRERAKPYKDHIHVIVRTPNGNDYGKDLLRLHYQQGHHASA
                     R"
     gene            complement(2270..3385)
                     /locus_tag="VE170_03545"
     CDS             complement(2270..3385)
                     /locus_tag="VE170_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14536.1"
                     /translation="MAIGLSHGGTNVYSAGERSTQLWVATQDGLALFERANDGWRETQ
                     RALRGQHISAIIFEQTSGTMFAGAFFGSIHASADGGKTWERRDNGMTIHDVYSLAAHV
                     VDGKVRVYAGTQPAHLFVSEDLGNHWSEIPSMREVPTTPQWSFPAPPHIAHTKFITFD
                     PHDAHTLYACVEQGAFLKSTDLGKSWRELNTVGLYKDKNRPVEHFYDVHRCLIDPRNP
                     ERIYVTGGAGLYVTHTGGGSWERWTADDWAKDVYPDGFVWNPKNPDTMFVAAAEHNPT
                     TWRKAGADARAEGKIYRSKDGGKNWQRLAGGLPASLKHEFGALCLEEANGDCAIFGGT
                     TGGEVYCSEDNGESWTLITDRLAPISKKGHERLLAAS"
BASE COUNT          670 a          991 c         1051 g          753 t
ORIGIN      
        1 tttcggcgag cttctgattg gtcgcgacgt agccctgaaa gggtagggtg aaaatatcgc
       61 ccatgaagac aagtttgcgc agcccttcac gttcggcggc gacggtgccg gcggggtcga
      121 gtacgacggc ggcaacgtta ccgcttttga ggccgaggat tttgtcgccg atgctcgtgc
      181 ctaatgagat tctagtgatc tccttggccg acacgccggc acgttcggca tacagcgtga
      241 tgaagcggtc ttcgctgtcg ccgatcaggg cgacgccgat tttcttgccc ttgaggtcgg
      301 atccctggct gatgcttgga tgaacgacta aataccacgt cggtttttcg gtttgataga
      361 agatcgcctt gagcggcgca ccgcgcacgg cgccgcgcaa gccgctgccg ccggcgccgc
      421 tgaagtcgac gtcgccgctg accaatgccg cgatgccggc cgggccgcca atggcgatgg
      481 tttcgttttc caagccttcg tcgcggaaga atttacgctc ctcggcgctg tacacgctca
      541 gataattgag attcgcgccg gggatggcgg tgcggatctt ttcggcgcgc gcgctggcgg
      601 cgagccaagc gacgcagaac aggacaagca agaaactaac acgtctgccg atcatggaac
      661 ctccaataag aagaatggcc gcaaagaacg caaagggcgc aaaaaagaat cgcgagccgt
      721 tgggggttga atcgcgcccc tgcgaaacat cgtgctcttg gtatccttcg tggtgaatac
      781 tctttcacac tacacctgga ggccccttac tttagaggcg agcagggttc gtcaggcatc
      841 atcgtgcgct cgcgtgatgc ccctgctggt agtgcaagcg cagcaggtcc ttgccgtagt
      901 cgttgccgtt gggtgtgcgc acgatgacat ggatatgatc tttgtacggc ttggcgcgtt
      961 cgcgaaaggc gatgccgcgt tgatggtcga actcgacgat gatcaccgga ctctggacgc
     1021 gataataaaa gacgccgtcg tcagcgccgc cgcccatcca gcaaaaatac gtatcgttca
     1081 aatggcgctt cacttcgtcc atcttgacct gggcatggcc gtcgcgcatg cgaccgacgt
     1141 gatattccac caagccgagc agtaactcat gttgtgcgct ggacaactcg ccggcgcgaa
     1201 tgccttggta atcaagcacc aggttgtcgc gaaacgcggt ggtaaagtct tcgccggaag
     1261 tctcgggcgc gattacagtt ttaacccgtt gcgattgtgt aagcgcgcgg aacaatgcca
     1321 gaccgcggtc ttcatcgccc ttgaatgcgc gcacgccggc gtatttgccg ccgacggcgt
     1381 gcaccggctc agcgcctagg aaagtcggcg tgacgacgat ctgctcgccg aggacgaaat
     1441 aattgatgat caaatgatgg ccatcccact gccagcccca gggttcagtc gccgacggcg
     1501 cgcccatgat gctcagccaa taaagatcct cgccgtattc atcgagccgg ccggtcaatt
     1561 ctaaaaccgt ttcattcaaa tgcatcagat cgagagtttc ttcgaagccc cgttggctca
     1621 agctctcgcg caacagcgca aaggcgcagt cgcgctggcg gtcgctcatc tcgaacagcg
     1681 ccatgccgtg gcgcaacaag gtcggatgga tgttgctcca tttgcgccac tggtctgtgt
     1741 ctacgggaaa gagcgtccga gcgcgttggt cggcgctcag cgtcgcgaga aacgcttcgg
     1801 ctgcttcgcg aatcaccttt gtcgacaggc cggtctgctg caggccgaag agatcggcaa
     1861 tgacatcgcc atcggcggta atccccttaa acggttcggc ggcggccgcc agcgatttct
     1921 cgatatagtt tggcggcccc cccttggtga agagcggtcg atgggaaccg cgttgattgc
     1981 aggtctcatg caaaccgtgg gtgcgtggat cgttcggcat agttggtctc cgaatcggtc
     2041 cgttttagtg cggacttgat ttagatgtca aggtagattt ggccaaccac catgggaaag
     2101 aaaaaccaga gcggcgccgg gtttgtccgt gggatcatct tggcggtttg ttcaccgggg
     2161 caattgcatt cggtcgttgc gtgctactct caagtcgatt tcttccttcc ttcgatggga
     2221 aagggagaag taatgctagc gcgagaagaa tcttttcagt ttttatatct caactcgccg
     2281 ccagcaaccg ctcgtgacct ttcttcgaga tcggcgccaa tcgatcggtg atcagcgtcc
     2341 agctctcgcc gttgtcttcg ctgcaataaa cctcgccgcc agtcgtcccg ccgaagatcg
     2401 cgcagtcgcc gttggcttct tcgagacaga gcgcgccgaa ctcatgcttc aagctggcgg
     2461 gcaggccgcc ggcaagccgc tgccagttct tgccgccgtc cttgctgcga taaatttttc
     2521 cttcggcgcg ggcatccgcg ccggctttac gccaggtcgt cggattatgc tcagcggcgg
     2581 cgacaaacat ggtgtcggga tttttcggat tccacacgaa gccgtcggga tagacatctt
     2641 tcgcccaatc gtcggccgtc caacgctccc agctgccgcc gccggtgtga gtgacgtata
     2701 acccggcgcc gccggtgaca tagattcttt cgggattgcg cggatcgatc aaacagcgat
     2761 ggacatcgta gaagtgttcc accggacggt ttttgtcttt gtacaggccc acggtgttga
     2821 gttcgcgcca gctcttgccc aaatcggtgc tcttcagaaa cgcgccttgc tcgacgcagg
     2881 cgtagagggt gtgcgcatcg tggggatcga aggtaatgaa tttcgtgtgg gcaatatggg
     2941 gcggcgccgg gaacgaccat tgtggtgtgg tcggcacttc gcgcatcgag ggtatttcac
     3001 tccaatggtt acccaaatct tcgctgacga acaaatgcgc cggttgggtg ccggcgtaga
     3061 cgcggacctt gccgtcgacg acgtgggcgg ccaggctata gacgtcgtgg atggtcatgc
     3121 cgttatcgcg gcgttcccag gttttgccgc catcggcgct ggcgtgaatc gaaccgaaga
     3181 acgcgccggc gaacatggtg ccggaggtct gctcgaagat gatcgcgctg atatgctggc
     3241 cgcgcagggc gcgctgggtt tcgcgccagc cgtcgttggc gcgctcgaat agcgcaagcc
     3301 catcctgggt ggcgacccaa agctgggtgg agcgttcgcc cgccgaataa acgttggtcc
     3361 cgccgtggga aagtccgatt gccatagatc acctccgcta tgcttgccgt aatttaccga
     3421 cccgcgatag gcgcactcta gcaaccgagg ttcttcgccg tcaat
//
LOCUS       DASQKS010000139         5473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3858635, whole genome shotgun sequence.
ACCESSION   DASQKS010000139 DASQKS010000000
VERSION     DASQKS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858635"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VE170_03550"
     CDS             <1..680
                     /locus_tag="VE170_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14537.1"
                     /translation="GGMLNSLAQTALKMTAPGVPDIFQGSELWDLSFVDPDNRRPVNY
                     SARANWLEELNAAASKDRLGLLEELLLHWHDGRIKLYLMSRLLDFRRAHAELFSAGEY
                     IPLNAVGAMDEQVCAFARRLGRVWAIAVVPRLMGRIAYHSGAPLGGELWGETTLDLPA
                     EAPRGWVDVISGESIEAAPTAALNRLSLASVFKHFPVALLCPENSIGSSPDVEEISRA
                     AIIQHNA"
     gene            673..2661
                     /locus_tag="VE170_03555"
     CDS             673..2661
                     /locus_tag="VE170_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HYJ14538.1"
                     /translation="MHEKSDRVVIEAVAPEIDGGRFPIKRVRGESVQVEADVLVDGHD
                     VISAILLFRRAQDAQWSETPMTPLVNDRWRSGFAVTEIGRYVYTLSAWVDRFKSWRRD
                     LRKKADAHTHTDLDMMTGALLVEEASRRAAGADAKKLAAWAKTLNDKNRGLAAKLADA
                     LSDELAALMENYPDRKFATTYDKELTVVVEREKARFSSWYEMFPRSCADQPGAHGSFK
                     DCERWLPYVASMGFDVLYLPPIHPIGRTQRKGKNNVVGSGADDPGSPWAIGAETGGHK
                     AIHPELGTVEDFKRLVKKAESLGIEIALDLAFQCSPDHPYVKEHPEWFRWRPDGTVQY
                     AENPPKRYQDIYPLDFDSEQWRELWQELRSVVQYWIEQGVNIFRVDNPHTKPLPFWQW
                     LIGDVKEHHPETIFLAEAFTRPKIMYNLAKLGFSQSYTYFTWRNTKPELMEYFTELSQ
                     TEVREYFRPNLWPNTPDILSEYLQFGGRPAFMIRCALAATLGANYGIYGPAFELLEAR
                     PRDPGGEEYLDSEKYQIQHWQRDSADSLKDFITRLNRIRHDNPALQSDWSLRFHETDN
                     EQIICYTKRAEDHDNIVAVVVNLDPHHVQSGWVKIPIESIDLNPLESYQAHDLLTGAR
                     FLWHGARNYVELDPRVVPAHIFRLRRRLRREQDFDYFM"
     gene            2667..>5473
                     /gene="treS"
                     /locus_tag="VE170_03560"
     CDS             2667..>5473
                     /gene="treS"
                     /locus_tag="VE170_03560"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HYJ14539.1"
                     /translation="MKVNHTRAQKSSLSDDPLWYKDAVIYELHVRAFADGNADGIGDF
                     EGLTEKLDYLHDLGVTALWLLPFYPSPLRDDGYDIASYTEIHPDYGTLKDFKLFLREA
                     HRRGLRVITELVLNHTSDQHPWFQRARRAAPGSKDRDFYVWSDSQEKYQDARIIFKDF
                     ETSNWAWDPIAKGYYWHRFYSHQPDLNFDHPDLKEAFFDVVDFWLGLGVDGLRLDAVP
                     YLFEREGTNCENLPETHQFLKELRQHVDQRFRDRMLLAEANQWPEEAVTYFGAGDECH
                     MAFHFPVMPRIFMAVHMEDRFPIVDILEQTPAIPDSSQWTLFLRNHDELTLEMVTDED
                     RDYMYKVYATDPKARLNLGIRRRLAPLLGNNRRKIELLNALLFSLPGTPVLYYGDEIG
                     MGDNIYLGDRNGVRTPMQWSADRNAGFSQANPQRLYLPIIIDAENHYEAVNVEAQQQN
                     LNSILWWTKRLIILRKRFHAFGRGTTEFLYPNNRRVLVFIRRLNDEVILTVANLSRFV
                     QCVEIDLAAFKGKSPVELFGQTEFPSIGDLPYFITVGPHGFYWFKIEAAQMAGTAEDR
                     QAPVIDIAGEWEQLITGGGRAALEKILPRYLRNRRWFGGKARRIHSTKITEAIPIPVD
                     STKAYFVMVEVGYVEGDPEIYILPLAYASGDRAAEICQHQADAVVAQFKATLPGPTGD
                     GILYDAVLDRKFLIALLEAVSRRRSYRGRSWQISAVPSKRFRGLRGAPASILEPVLTK
                     TEQSNTSIRYGDRLILKIFRRLHEGLNPDLEIGRFITERTTFPNVPPLAGSLEFGKGK
                     KAVATVGILQGWVANEGDAWNYTLDHLTDYFENCLARSATPIDLARPPGSVLDNLDQD
                     VPALALEMIGAFLTSASLLGQRTGELHMALASDTQDPNFATEPFTQFYRRSLYQSLRT
                     TTDRALTLLRERLPTLPEDTRA"
BASE COUNT         1169 a         1559 c         1569 g         1176 t
ORIGIN      
        1 gcggcggcat gctcaactca ttggcgcaaa ccgcgctgaa aatgaccgca ccgggggtgc
       61 cggacatctt tcaaggcagc gagctttggg acctaagttt cgtcgatccg gataatcgcc
      121 gtccggttaa ttacagcgcg cgcgcgaatt ggctggagga gctcaatgcc gccgcgtcca
      181 aggaccggct gggcttgctc gaggaattgc ttttgcattg gcatgacggt cgcatcaagc
      241 tctatttaat gtctaggctt ttggattttc gccgtgccca tgccgagctt tttagtgccg
      301 gcgagtacat tccgctcaac gccgtcggcg cgatggacga gcaagtgtgc gcctttgcca
      361 gacgcctcgg tcgggtctgg gcgattgcgg ttgtgccgcg cctgatgggg cggattgcct
      421 atcacagcgg cgcgccgcta ggcggggaac tatggggcga aacaacgctc gatctgcccg
      481 ccgaggcgcc gcgtggctgg gtcgacgtga tcagcggtga atcaatcgaa gcagcgccga
      541 cggcagcgct aaatcgtctc tcgctggcaa gtgtgttcaa acattttccg gtggcattac
      601 tttgtcctga aaattccatc gggtcgtcac cggatgtcga ggaaatctcg cgtgcagcca
      661 ttatccaaca taatgcatga gaaatccgat cgcgtggtta tcgaagccgt cgccccggaa
      721 attgacggcg gacggtttcc aatcaaacgc gtgcgcggcg agtcagtgca ggtcgaagcc
      781 gacgttctcg tcgacggcca tgacgtgatc tcggctattc tcctgtttcg ccgcgcccag
      841 gacgcacaat ggagcgaaac accgatgacg ccgctggtta acgaccgttg gcgcagtggg
      901 ttcgccgtca ccgagatcgg gcgctatgtt tatacgctga gcgcgtgggt cgatcgtttc
      961 aagtcgtggc gccgggatct gcgcaagaaa gccgacgccc acacgcacac cgacctggac
     1021 atgatgaccg gcgcattact ggtcgaggaa gcgagccggc gcgctgccgg cgcggacgcg
     1081 aaaaagctcg ccgcatgggc aaaaacttta aacgacaaga atcgaggact ggcggcaaag
     1141 ctcgccgatg ctctgagcga cgagctcgct gccctgatgg aaaattatcc cgatagaaaa
     1201 ttcgcgacca cttacgacaa ggaattgact gtcgtggttg aacgcgaaaa ggcgcgcttt
     1261 agctcgtggt acgagatgtt tccgcgctcg tgcgcggacc agcccggcgc gcacgggtcc
     1321 tttaaagact gtgaacggtg gcttccttac gtggcgtcga tgggcttcga tgtgctctat
     1381 ttgccgccga ttcatccgat cggacggacg cagcgcaagg ggaaaaataa cgtggttggc
     1441 agcggcgccg acgacccggg cagtccgtgg gcgatcggcg ccgagacggg cggtcacaaa
     1501 gccattcatc cggagctcgg cacggttgag gattttaaac ggttggtcaa aaaggccgag
     1561 tcgctaggca tcgagatcgc cttggatttg gcgtttcaat gcagccccga ccacccgtat
     1621 gtcaaggagc acccggagtg gtttcgttgg cgtcccgacg gcaccgtgca gtacgccgaa
     1681 aatccgccaa agaggtacca ggacatttac cccctcgatt ttgactcgga gcagtggcgc
     1741 gagctctggc aagaattacg aagcgtggtg cagtactgga tcgagcaagg ggttaatatc
     1801 tttcgggtcg acaatccgca taccaagccg ctgccgtttt ggcagtggct gatcggcgac
     1861 gtgaaggagc accatccgga gacaattttt ctcgccgagg cgttcacgcg tcccaagatc
     1921 atgtacaact tggctaagct cggtttcagc caatcctata cctatttcac ctggcgcaat
     1981 accaagcccg aattgatgga gtactttaca gagctcagcc agaccgaagt gcgggagtac
     2041 tttcgtccca acctatggcc gaatacgccc gatatcctga gcgagtatct ccagttcggc
     2101 ggccgcccgg cttttatgat ccgatgcgct ctcgccgcga ctctcggcgc taactacggc
     2161 atctatggtc cggcgttcga actgctcgaa gcgcgcccgc gcgaccccgg cggcgaggaa
     2221 tatctcgact cggagaaata ccagatccaa cattggcagc gagacagcgc cgacagcctt
     2281 aaggatttca tcacgcgact taaccggatc cgccacgaca acccagcgct acaaagcgat
     2341 tggagtctgc gcttccatga aaccgataac gagcagatta tttgttacac caaacgggca
     2401 gaagatcacg acaacatcgt cgccgtggtg gtgaacttgg atccgcacca cgtgcagtcc
     2461 ggttgggtga aaattcccat cgagtcgata gatctcaatc cgctggaatc ttatcaggcc
     2521 cacgatcttt taaccggcgc ccgctttctc tggcatggcg cgcgcaatta tgtcgagctc
     2581 gatccgcgcg tcgtgccggc gcatattttt cggctgcgcc ggcgcctccg gcgcgaacag
     2641 gattttgatt attttatgtg acgaccatga aagtgaacca cacccgcgcg cagaaatctt
     2701 ctctcagcga cgatccgctc tggtacaagg acgcggtgat ttacgagctg catgtccgcg
     2761 ccttcgccga cggcaacgcc gacggtatcg gtgattttga ggggctcacg gaaaagctcg
     2821 actatctgca cgatctcggc gtgacggcgc tttggttgtt gccgttttat ccttcgccac
     2881 tgcgggacga tggttacgat atcgcgagct acactgaaat tcatccggat tacggcacgc
     2941 tcaaagattt caaacttttc ttacgcgaag cgcaccggcg cgggctccgc gtgatcaccg
     3001 agttggtgct caatcacacc tccgatcagc acccttggtt tcagcgcgcc cggcgcgccg
     3061 cgcccggcag caaagatcgc gacttctatg tctggagcga ttcccaagaa aaataccagg
     3121 acgcgcgcat tatcttcaaa gacttcgaga cctccaactg ggcctgggat cccatcgcca
     3181 agggatatta ctggcatcgt ttttactcgc accagccgga tctcaacttc gaccatcccg
     3241 acctcaagga ggcgtttttc gacgtcgtcg acttttggtt gggattgggg gtcgacggtt
     3301 tacgactcga cgcggtgccg tacctgttcg agcgcgaagg caccaactgc gagaatcttc
     3361 cggaaaccca tcagttcttg aaggagttgc gccaacacgt cgatcagaga ttccgcgatc
     3421 gaatgctgct cgccgaggcg aaccagtggc ctgaggaggc ggtcacctac ttcggtgccg
     3481 gcgacgaatg ccacatggct tttcatttcc ccgtgatgcc gcggattttt atggccgtgc
     3541 atatggaaga ccgctttccg atcgtcgata tcctcgagca gaccccggca attccggaca
     3601 gcagccagtg gaccctgttc ctgcgcaacc atgacgagtt gacactcgaa atggtgacgg
     3661 atgaagaccg cgactatatg tacaaagtct acgccaccga tcccaaggcg cggctcaatc
     3721 tcggcattcg ccgccgtttg gcgccgctgc tgggtaataa tcggcgcaag atcgagttgc
     3781 tcaatgcgtt gctgttctcg ttgccgggca caccggtgct ctattacggc gacgaaatcg
     3841 gcatgggcga caatatctat ctcggcgacc gcaacggtgt gcgcacgccg atgcaatgga
     3901 gcgcggaccg caatgcgggc ttctctcaag ccaatccgca gcggctttat ctgccgatca
     3961 tcatcgatgc tgaaaaccat tatgaggcag tcaacgtcga agcgcagcag caaaacctga
     4021 actcgatcct atggtggacc aagcgcttga tcatcctgcg aaaacgcttt cacgcattcg
     4081 ggagaggtac aaccgaattt ctctacccaa acaatcgccg cgtcctggtt ttcatccgcc
     4141 gtctgaatga cgaagttatt ctaacggtgg caaatctgtc gcgcttcgtc cagtgcgttg
     4201 agatcgacct cgcggcgttt aaagggaaat cgcctgtgga gcttttcggc cagaccgagt
     4261 ttccatcgat tggcgatctg ccctacttta tcaccgtggg tccccatggt ttttattggt
     4321 tcaagatcga ggcagcgcag atggcgggca cggcagagga caggcaagcg ccggtcattg
     4381 atatcgccgg tgaatgggag caactgatca ccggtggagg cagggcggca ttggaaaaaa
     4441 tattgccgcg gtatctccgc aaccggcggt ggtttggtgg caaagcacga aggattcatt
     4501 caaccaagat cactgaagct attcctatac ccgtcgattc taccaaagcc tattttgtca
     4561 tggtcgaggt aggctacgtc gaaggcgatc ctgaaattta tattttgccg cttgcgtacg
     4621 ccagcggtga ccgcgccgcc gaaatctgcc aacatcaagc ggacgcagtc gtcgcgcagt
     4681 tcaaagcgac gctgccgggg ccgaccggtg acggcatact ctatgacgcg gtcctggatc
     4741 gcaagtttct catcgcgctg ctcgaagcgg tatcgcgccg gcgcagttat cgcggtcgtt
     4801 cgtggcagat ctcagcagtt ccatcgaaac gttttcgcgg cctcaggggg gcaccggcgt
     4861 cgattttgga acctgtcctg acaaaaactg agcagagcaa tacttcgatt agatacggcg
     4921 accggttaat tttgaaaatt tttcggcgat tgcacgaagg gctcaatccc gatcttgaga
     4981 tcggccgctt catcacggag cgaaccactt ttcccaatgt tccgccgctg gcaggtagtc
     5041 tggagttcgg taaaggcaag aaagcggtgg cgaccgtcgg gatattgcaa ggctgggtcg
     5101 ccaacgaagg tgatgcgtgg aattataccc tcgatcatct taccgactat ttcgaaaatt
     5161 gcctggcgcg tagcgcgacg cccattgact tggcgcgtcc gccgggcagt gtattggata
     5221 atctggatca ggacgttcca gccttggccc tggaaatgat cggcgctttt ctcacgtcgg
     5281 cgagtctttt aggtcagcgc accggcgagt tgcacatggc actcgcctcg gacacgcaag
     5341 atcccaattt tgccactgaa ccatttacgc agttctaccg ccggtccttg tatcaaagtt
     5401 tgcgcacgac aacggatcgc gcgttgacac tgcttcgcga gcgtctccca accttgcccg
     5461 aagatacgcg cgc
//
LOCUS       DASQKS010000140         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3859317, whole genome shotgun sequence.
ACCESSION   DASQKS010000140 DASQKS010000000
VERSION     DASQKS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859317"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VE170_03565"
     CDS             <1..728
                     /locus_tag="VE170_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14540.1"
                     /translation="AMTALLVIPPVLFFLPRDAKAGGPQVAAAKSVTDQSFDHDWTVK
                     QALTNSAFWLLFIARTLASLGNQVIVTHQIAHAVDVGYSKVFAASIFGFMGVVSIGGR
                     ILFGYLADVMNRQMVFTVVQIISAFGIFALLALSDASSPWLLYSYAMCYGLGQGSRAL
                     VLSAISADIFHGRHFGAIFGYFTFSIGLGGAIGAWLGGFLFDITHSYSIPFWVSLACL
                     IISVFVVWASSRVVNNQFAMERK"
     gene            732..1349
                     /locus_tag="VE170_03570"
     CDS             732..1349
                     /locus_tag="VE170_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ14541.1"
                     /translation="MIQLYTWGTPNGKKASIMLEEVALPYEVHAVNFGQNDQFKPEYL
                     AINPNNKIPAIIDPDGPGGKPLKLFESGAILMYLAEKSGKLWPADLAQRYTVIQWLMF
                     QMGGVGPMFGQANYFFRLEEKIPYAIERFHKEAKRLYNVLNQELGRHEYLAGEYSIAD
                     IATYPWVWRHDGHHVKLEEYPNVKRWYEQIGQRPAVQRGMEIPKK"
     gene            1409..3187
                     /locus_tag="VE170_03575"
     CDS             1409..3187
                     /locus_tag="VE170_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14542.1"
                     /translation="MAKQQKVEKPRAEWGSDVIVDAIKAFEIEYIAINPGATFRGLHD
                     SLVNYGGNHAPEIILCNHEEIAVALAHGYARAKAKPMAAAVHDVVGLQHASMAIYNAW
                     ADRLPVIVLGGTGPMDSANRRPWIDWVHTANVQGNLVRDFCKWDDQPASVEAVADSFI
                     RAYRLATTDPMGPVYICYDGDVQEKKLDSELTFPSLARYPAPLPLQAPEEGFAQTIEM
                     ILAAKRPVILADWVGRREAGFNALRELAELLAAPVLDQLGRFNFPVLHPLNLTGQADK
                     VIPQADLILALDVPDLEGATVRRAQELGNRKATPILPDDAKVINVALDDLLVRAWATD
                     FNKLRQADLMILADTAVFLPELLRRLKAEKKLGDLIDDIARRRADWGNIYEEKRAALD
                     KELTAKWDDKPVSMTRLYAEIAEALKGEDWVLTNGSSQGKENLYLPATKFNQILGKYK
                     GGGLGYGMPASLGAALAHKSSGKFCLNIQPDGDLLFTISSIWTAVHHQIPLLSIVCSN
                     RSYFNDEEHQERIAIQRKRPVENKTIGIRIEDPNVDFAGVARNYGCWGAGPITEPKDL
                     IKTLREAVKVVKAGKPALVDVVCQMR"
     gene            3370..>3746
                     /locus_tag="VE170_03580"
     CDS             3370..>3746
                     /locus_tag="VE170_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14543.1"
                     /translation="MGVPIGWFAGSPPTVEQSAISPAKATTYSRLSSVELKNQSAQLV
                     TAIRGLARSYYDEERRLRSLADENSGKTESQAERERIRTAWLADSAKLHDMFLDRYNT
                     EFWADAVLLRQAIVAKVGAAPGA"
BASE COUNT          788 a         1031 c         1109 g          818 t
ORIGIN      
        1 cggcaatgac cgcgttgctg gtgattccgc cggtgctgtt ttttttgccg cgcgatgcca
       61 aggctggcgg acctcaagtt gccgcggcga aatctgttac ggatcaatcg ttcgatcacg
      121 actggacggt caagcaggcg ctaaccaatt cggcgttttg gcttttattc attgcccgga
      181 cgctggcgag cctgggcaat caagtcatcg tcacgcacca aatcgcccac gctgtcgacg
      241 ttggctacag caaagtattc gcagcgtcga ttttcggttt catgggagtc gtcagcatcg
      301 gcgggcgcat tcttttcggt tatctcgccg acgtcatgaa tcgtcaaatg gtctttaccg
      361 tggtacagat tatttccgcc tttggcatct tcgcgctgct cgcgttatcc gacgcgtcgt
      421 cgccctggct gctttacagc tatgcgatgt gctacggact ggggcaaggt tcgcgcgcct
      481 tggttttgtc ggcgatttcc gccgacattt ttcacggtcg acattttggc gcgatcttcg
      541 gctactttac atttagcatc ggccttggcg gcgccattgg cgcctggctc ggcggctttc
      601 tcttcgacat cacgcatagc tattcgattc ccttttgggt ttcgcttgct tgcctgatca
      661 tttcggtatt tgtcgtttgg gccagcagtc gcgtcgtcaa taatcaattt gccatggaaa
      721 ggaaatgaat catgattcaa ctctacactt ggggcacacc caacggaaaa aaagcttcga
      781 tcatgctgga agaggtcgcc ttaccttacg aagttcacgc cgtcaatttc ggccagaacg
      841 atcagttcaa accggaatac ctggcgatca atcccaacaa caaaattccc gcgatcatcg
      901 atccggacgg ccccggcggc aagccgctca agcttttcga atcgggtgcg attctcatgt
      961 acctggcgga aaaatcaggc aagctctggc ccgccgacct ggcacagcgc tataccgtga
     1021 ttcaatggct gatgtttcag atgggcgggg tcggaccgat gtttggccag gccaattact
     1081 ttttccgact ggaagaaaaa attccctacg ccatcgagcg cttccataag gaagcgaagc
     1141 ggctttacaa cgtgctcaac caggaactgg gccggcatga atatctcgcc ggggaatact
     1201 cgattgccga catcgcgacg tacccttggg tgtggcggca tgacggccat catgtgaagt
     1261 tggaagagta tcccaatgtg aaacgctggt acgaacagat cggccaacga ccggcggtgc
     1321 agcggggcat ggaaattccg aaaaagtaga tagcctgatc atgttcgtgg cgggtgcttc
     1381 tgatggttgc acgtgaaagg gaaaagccat ggccaagcag cagaaagtcg aaaagccgcg
     1441 cgccgaatgg gggtcggatg tcatcgtcga tgcgatcaaa gccttcgaga tcgagtatat
     1501 cgcgatcaat ccgggggcaa ccttccgcgg cctgcacgat tcgctcgtca actacggcgg
     1561 taaccacgcg ccggagatca ttctctgcaa ccacgaagag atcgccgtgg cattggcgca
     1621 tggctatgct cgggccaaag ccaagccaat ggccgccgcg gtgcatgatg tcgtcggtct
     1681 gcagcatgcg tcgatggcga tctataacgc ctgggctgac cggctcccgg tcatcgtcct
     1741 gggaggaacc gggccgatgg attcggccaa ccgccgcccc tggatcgact gggtccatac
     1801 cgccaacgtt caaggcaatc tagttcgtga tttctgcaag tgggacgatc aaccggcgag
     1861 tgtcgaggcg gtggccgatt cctttatccg cgcctatcgg ctggcgacca ccgacccgat
     1921 ggggcccgtt tatatctgct acgacggcga tgtccaggag aaaaaattgg acagcgaatt
     1981 aacctttccg tcgttggctc gatatcccgc gccgctgccg ctgcaagcgc cggaagaagg
     2041 cttcgcacaa accattgaga tgatcctagc ggccaagcgg ccggtgatct tggcggactg
     2101 ggtggggcgg cgcgaggctg gtttcaacgc gcttcgagaa ttggccgagc tgctcgccgc
     2161 gcccgtgctg gatcagttgg gccggttcaa ttttcccgtt ttgcatccgc tcaatttgac
     2221 cggtcaagcg gacaaagtaa taccgcaggc ggatctgatt ttagctctgg atgtgccgga
     2281 ccttgaaggc gccaccgtgc gccgtgccca ggaactcggc aaccgcaagg cgacgccgat
     2341 cttgccggat gacgccaaag ttatcaacgt cgccctcgac gatctgctgg tgcgcgcctg
     2401 ggccaccgat ttcaataaat tgcgccaagc ggatctgatg attctcgccg acaccgcggt
     2461 ttttctgccg gaactcttgc gccggctcaa ggccgaaaag aagctcggcg atttaattga
     2521 cgacattgcc aggcgccgcg ccgattgggg caatatctat gaagagaagc gagcagcgct
     2581 cgataaagaa ttgacagcga agtgggatga caagccggtg tcgatgacac gcctctacgc
     2641 cgagatcgcc gaagcgctca aaggggagga ctgggtgctg accaatggca gctctcaggg
     2701 caaggagaat ctttatttgc cggcaaccaa gttcaatcag atcctgggca agtacaaagg
     2761 cggcggcttg ggctatggca tgccggcgtc gttgggcgcg gcgctggcgc ataaaagctc
     2821 gggcaagttt tgcctcaata ttcagcccga cggcgattta ttattcacca taagttctat
     2881 ttggaccgcg gtgcatcacc agattccctt actcagcatc gtctgcagta atcggtccta
     2941 tttcaacgac gaggagcacc aggagcgcat cgccatccag cgcaaacgtc cggtggagaa
     3001 caagaccatc ggcatccgca tcgaagaccc taatgtcgac ttcgccggtg tcgcgcgcaa
     3061 ctacggctgc tggggcgccg ggccgatcac cgagccaaag gatctgatca agacgctgcg
     3121 cgaagcggtg aaggtggtga aggcaggcaa gccggcactg gtcgatgtgg tgtgccagat
     3181 gcggtgattt aaccgttggg aaagcgagca gtactggcgc gcgttcgctg gatcgccatt
     3241 gcggctccat ttttctcacc gcgtctggcg cggatttttc aatcttgaac taaaatgttt
     3301 ggttccctgg gcaaattctg gagtgtagcg aaacggcatc ctctcgtggt gcttctggcg
     3361 ggtctggttg tgggggttcc gatcggctgg tttgccggct caccaccgac ggtcgagcaa
     3421 agcgcgatat cgccggccaa agcgacgact tattcaaggc tctccagcgt ggaacttaaa
     3481 aaccagtcag ctcaattggt gacggcgatt cgcggcctgg cgcgatccta ttacgatgaa
     3541 gagaggcggt tgcgaagctt ggcggatgag aactcgggca agaccgaatc gcaagccgag
     3601 cgggagcgaa ttcgcaccgc atggctcgct gacagtgcca agctgcatga catgtttctc
     3661 gaccgctata atactgagtt ttgggccgat gcggtgctgc tgcgccaagc catcgtcgcc
     3721 aaggtggggg cagcgccggg ggcgca
//
LOCUS       DASQKS010000141         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3019780, whole genome shotgun sequence.
ACCESSION   DASQKS010000141 DASQKS010000000
VERSION     DASQKS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3019780"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1329)
                     /locus_tag="VE170_03585"
     CDS             complement(247..1329)
                     /locus_tag="VE170_03585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14544.1"
                     /translation="MEIIDADGHVNDIADQEAIAKYMPAGNRSSSIFPVLDHLHFHYL
                     RPSGNRPGLENPGPQEWVQFLDETGISSSVLYPTFGLAVGRFVSAHWAIAACRAYNNW
                     LYEKFTSKNLRLKGMALLPIQDVDATVLELRRAVKEFGMLGGMLPSNGEGIKDHLGAK
                     MYWPIYEEAEKLGCALAVHVGSLHHLGMDGFSTYYPVHALGHPFGIAIQAAGLLAHGI
                     FERFPGIRVAFLEGGSTWVPFFLDRLDRSYHPGHLQVDFEDQPVGGPRPGEKASDYLR
                     RQMREGRIYVGFDVDDDGLGFAVQKAGRDAFVFGSDFPHEVFNAEKCRHEIDQLLARG
                     DLSSTDKAAVLGGNAKRFYGLGANGG"
     gene            1547..3616
                     /locus_tag="VE170_03590"
     CDS             1547..3616
                     /locus_tag="VE170_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYJ14545.1"
                     /translation="MSKYRVTVDTGGTFSDFVFFNEDSGEIAITKVPSTPKEPFQAVL
                     NGVKELIDQGVAAGDISFFCHGTTVGTNALLEEKGAKTGLMVTQGFRGIYEVMEQTRG
                     YGPATYDLFFEKPRLLAPPYLTEEIPERVDFKGNVLKAIDVEASRQAIRRLKKKGVQS
                     VAVCCLFSFLNPDHELKIKELFAQEFPEARLSLSCEVLPQIREFYRLSTTVINGYIQP
                     VMASYLGRLENRMREMGVVTPKLYIMQSNGGVSTFKGSAEKPVATVLSGPAGGVIASM
                     GTCERVGIDNIITFDMGGTSCDVALIHEGNPVITTQGKINLRPIALPMLDIHTVSAGG
                     GTIARIDAVGGLQVGPDSAGADPGPVCYDRGGENITVTDANMVCGVLDPDHFLGGRMK
                     LDKAKAQRVLEEKLAKPLKLDVLEAADGVLKIIDAKMEEAIKAVSSARGYDIRDFTLV
                     AFGGGGPMHAGRMALDLGIPSVLVPLTPGVHSALGLLMSDVKHDYVRSKLVGLEDLDL
                     TEINHMFHQLIDRAKADLVSEGFTDDEIKIEPYLDLRYAGQGYELTVPSPMPPYTQHD
                     LTVMRGRFDTMHEQNSGHKAESEPVELVSLRLISLGLVPQAKLSPGKITGRKVDQAKL
                     GERKVYFGKEHGVLTTQVYQRDLFEPGHQITGPAIVEQLDTTTVIQPEQEAVVDKYRN
                     IIIEKKS"
BASE COUNT          795 a         1088 c         1071 g          718 t
ORIGIN      
        1 gttccaatgg gccccggata tctctttgag ctcaccgccg gcgcggactt cgtcaataaa
       61 agtccgcagg tcgagagccg cttgcaggcg ctgttggtta ttcatcgtcg tggtattgct
      121 ccactctagg caagattgcg ctgcgcacgc tagcgaagtt taccagcact ctgcgcggcg
      181 cgcgcaaaat ttccggtgat ctggaaaact caaagaagag attgactccc gtcaaatgcc
      241 aacaaactat ccgccattgg cgcccaaccc ataaaatcgc tttgcgttgc cgccgagcac
      301 cgccgctttg tcggtactgc tgagatcgcc gcgcgcgagc agctggtcga tctcatgacg
      361 gcatttttcg gcgttgaaaa cctcgtgggg aaagtcactg ccgaagacaa acgcgtcgcg
      421 cccggctttt tgcacggcga agcccaagcc atcgtcgtcg acatcgaagc cgacatagat
      481 gcgcccctcg cgcatttgcc ggcgcaggta atcgctcgcc ttctcgcccg gccgcggccc
      541 gccgaccggt tgatcctcga agtctacttg cagatggccg ggatgatagg aacgatcgag
      601 gcgatcgagg aaaaatggca cccaggtcga gccgccttca agaaaggcaa cgcgaatgcc
      661 gggaaagcgc tcgaagatac cgtgggcgag caggccggcg gcttgaatgg cgatgccaaa
      721 gggatgaccg agggcgtgca ccggataata ggtgctaaaa ccgtccatgc cgagatggtg
      781 cagcgacccg acatgcaccg ccaaggcgca gccgagtttc tccgcctcct cgtagatcgg
      841 ccaatacatc ttggcgccga gatgatcctt gatgccctcg ccgttggacg gcagcatgcc
      901 gccaagcatg ccgaattctt tcaccgcgcg gcgcaattcg aggacggtgg cgtcgacatc
      961 ttggatcggc aatagcgcca tccctttgag ccggaggttt ttgctggtga acttttcgta
     1021 gagccagttg ttgtaggccc ggcaggcggc gatcgcccag tgcgctgaaa caaaccggcc
     1081 tacggcaagg ccgaatgtcg gatagagaac gctggacgag atcccagtct cgtcgagaaa
     1141 ctggacccac tcctgcggtc cgggattttc cagcccgggg cgattgccgg atggccttag
     1201 gtagtgaaag tgcaggtgat cgagcaccgg aaagatcgat gacgagcgat tgcccgccgg
     1261 catatatttg gcgatcgcct cctgatcggc gatatcgttg acatggccat cggcgtcgat
     1321 gatttccata gtctcccctc gttacgtgag cagcgctgat tgccggcgtg atggatgcgc
     1381 cggttatcta tcctcggcca ggacgatgac ccagctgtaa aacagactcg ttgtaagcac
     1441 aagagctggc gcagttgcgc gcgccggtct gccgcgcgtc acaacgacgt tacgcagcgg
     1501 tatcgagatt gaaggcgtga aaattacatg gtagacgaaa cggactatgt ccaagtaccg
     1561 agttaccgtc gacaccggcg gcaccttttc cgattttgtt ttcttcaacg aagatagcgg
     1621 cgagatcgcc attaccaaag ttccgtcgac gccgaaggag ccttttcaag cggtgttgaa
     1681 cggcgtcaaa gaactgatcg accagggcgt ggccgccggc gatatttcat ttttctgcca
     1741 tggcaccacc gtcggcacca atgcgttgct cgaagaaaaa ggcgccaaga ccggcttgat
     1801 ggtgacccag ggctttcgcg gcatttacga agtcatggag cagacccgcg gttatggccc
     1861 ggcgacctac gatctgtttt tcgagaagcc gcgcttgctc gcacctccct atttgaccga
     1921 agagattccc gagcgcgtcg atttcaaagg caacgtgctc aaagcgatcg acgtcgaagc
     1981 atcgcgccaa gcgatccgcc ggctcaagaa aaaaggcgtg cagtcggtgg cggtgtgctg
     2041 tttgttttcg tttttgaatc ccgaccatga gctcaagatc aaagagctgt tcgcccagga
     2101 gtttcccgaa gcgcggctgt ctttatcctg cgaagtgctg ccgcaaatcc gtgagttcta
     2161 ccgcctgagc accacggtga tcaacggcta tatccagccg gtcatggcga gctatttagg
     2221 ccgcctggag aaccgcatgc gggagatggg cgtggtcacg cccaagcttt acatcatgca
     2281 atccaacggc ggtgtttcga ccttcaaagg ctcggcggaa aaaccggtgg cgacggtgct
     2341 ttccggcccc gccggcggcg tcattgcgtc gatgggtact tgcgagcggg tcggcatcga
     2401 caacatcatc accttcgaca tgggcggcac cagctgcgat gtcgcgctga tccacgaagg
     2461 caacccggtg atcaccaccc aggggaaaat taacctgcgc cccatcgccc tgccgatgct
     2521 cgacattcac accgtcagcg ccggcggcgg cacgatcgcg cgcatcgacg cggtgggcgg
     2581 cttgcaggtg gggcccgaca gcgccggcgc cgatcccgga ccggtgtgct acgaccgcgg
     2641 cggcgaaaac atcaccgtca ccgatgccaa tatggtctgc ggcgtcctcg atcctgatca
     2701 tttccttggc gggcgcatga agctcgacaa ggcgaaggcg caacgggtcc tggaggaaaa
     2761 gttagccaag ccgctgaagc tcgatgtcct cgaagccgcc gacggcgtct tgaaaatcat
     2821 cgacgccaaa atggaagaag cgatcaaggc ggtgtcgtcg gcgcgcggct atgacatccg
     2881 cgactttacc ctggtcgcct tcggcggcgg cggaccgatg cacgcgggtc gcatggcgct
     2941 ggatttggga attccctccg tgctcgtgcc gctcaccccg ggtgtccatt cggcgctggg
     3001 attgctgatg tccgacgtca aacatgacta tgtgcgctcc aaactggtgg gcctggaaga
     3061 tttagacctc accgagatca accatatgtt tcatcaactg atcgatcgcg ccaaggccga
     3121 tctcgtgagc gaaggcttca ccgacgacga gatcaagatc gaaccctatc tcgatctgcg
     3181 ctacgccggc cagggctacg agctcaccgt cccctcgccg atgccgccat acacccagca
     3241 tgatctgacc gtcatgcgcg gccgtttcga caccatgcac gagcagaatt ccggccataa
     3301 ggccgagagt gagccggtgg agctggtgag tttgcgcttg atctctctgg gcttggtgcc
     3361 acaggccaaa ctatcacccg gtaaaatcac cggccgcaaa gtcgaccaag ccaagctcgg
     3421 tgaacgcaag gtttatttcg gcaaagagca tggcgtgctc accacccagg tataccaacg
     3481 cgacctgttc gagcccggcc accaaatcac cggcccagcc atcgtcgagc aattggacac
     3541 cacgacggta attcaacccg aacaagaagc tgtcgtggat aaatatcgga atatcatcat
     3601 tgaaaaaaaa tcatgatcga ctccggcgac ttgaacgttt tgaacgtttt gaccggctgg
     3661 aacctcagcg cg
//
LOCUS       DASQKS010000142         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5117432, whole genome shotgun sequence.
ACCESSION   DASQKS010000142 DASQKS010000000
VERSION     DASQKS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5117432"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VE170_03595"
     CDS             <1..247
                     /locus_tag="VE170_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14546.1"
                     /translation="KIKNARQEIVELLKATIEGLDYTWVQREGTIEIIAKWMKLNPIQ
                     AAKAYDSVRDTFSKIGVPTEEQTKAYIAMLSSTASLK"
     gene            606..1742
                     /locus_tag="VE170_03600"
     CDS             606..1742
                     /locus_tag="VE170_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14547.1"
                     /translation="MYRRPETKAAEEKVYKQQQIGEFMTTWKDFPRPGLAAWAERRKA
                     GLMGTAKSTHYTPRFRPFDMLSIPNQSLVILENEYHRIGAESVVGVQDSFHRYVDSDM
                     VYFQFCGGSKVETEFGVYEMAPGDVMLVPGGIAHRSIGRNDSLRYFCQSHEAVDYVMN
                     EDQYTSQTSFEVKRLGGPNWTAAQGTNAPGKGPVIEKMHFWDDGPEDQTVVERDYESL
                     VGIAGIGRGKPGSLVRKLRAFDHFTAIVGKGREDAGTQPLMESANMRIRTYNMQDEQF
                     AFHRALRSEEVRIQFRGDALDLSEFENVEVSPGEITIIPLGISHSVISIPPEDENFLR
                     LNFYSKVRWRVPIDPTKHYFDSKFEIATTVHKQAEWRKKLAAAR"
     gene            1894..2172
                     /locus_tag="VE170_03605"
     CDS             1894..2172
                     /locus_tag="VE170_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14548.1"
                     /translation="MKIYGVRWRDGSFSVTLAKNKLHALEEFDKIADANGLKIEEFDE
                     FLATFKLTESGDIVLEEIDSDTRGELGRVARNRKRPSQCSKKRIGARR"
     gene            2445..2624
                     /locus_tag="VE170_03610"
     CDS             2445..2624
                     /locus_tag="VE170_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14549.1"
                     /translation="MAKRSQPVSGSRIYPIISGKERLAIWEKARGMWKKRGSDPIQEL
                     KKMRAPWVTKRSFLK"
     gene            2625..2996
                     /locus_tag="VE170_03615"
     CDS             2625..2996
                     /locus_tag="VE170_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYJ14550.1"
                     /translation="MYTLDTNAILYYLKDDNDAVAILRGVFAQSSPVYVSAVTELELL
                     AFSNLTSDEELLIENLLATVAIIPVDSHIARLAALVRRRYRIKVPDSIIAATAMFTGS
                     ILLTRNTRDFRKISNLVIRKI"
     gene            3076..>3575
                     /locus_tag="VE170_03620"
     CDS             3076..>3575
                     /locus_tag="VE170_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14551.1"
                     /translation="MTISFRPRWYFFIALVSVTFIGPLSLHLFIPAMPAVKEAFGVST
                     GMAQLTMSLAMFSMAFFTVAYGGLSDRFGRKRVLLGGLVLFTCGAASCMAAANMPMLL
                     AGRILQGAGAGCGVVLARAIARDVYGQDRVAQVIAYLTAAYVLGPMLAPPIGGHLTVL
                     FGWRAL"
BASE COUNT          835 a          989 c         1020 g          731 t
ORIGIN      
        1 aaaaatcaaa aacgcgcgcc aggaaatcgt cgagctcctc aaggcgacga tcgaaggact
       61 cgattacact tgggtgcaac gcgagggaac catcgagatc atcgccaagt ggatgaagct
      121 caatccgatc caagcggcca aggcgtatga ctcggtgcgc gacactttct caaaaatcgg
      181 cgtgcccacc gaggagcaga cgaaagcgta catcgcaatg ctcagctcga cggcgagttt
      241 gaagtgagat gttgccgccg gatcgatctt cgatttctcg ctggccgcgg agacggccaa
      301 agaaatggcg cagaaaaaat agcgcgaaga ggatggcaat gattgtcaga gtcagagggg
      361 aaggtttgct atcgattacg cgccggcaag cgaaccaagc gcgctcctgc agcgccgatc
      421 gtcttcgcag ccggccatct cattcatcgc cgcattatag ccagcacgtt ttatctgatc
      481 ggtcggtttg tctcagggtt tgccggaagg tcgccggcgc aggacttcgc cttgacaatc
      541 cgccggacct tctgtagctc accttcataa ccatattaac gccagtcatt gcgcacggag
      601 gcatcatgta tcggcgaccg gaaaccaaag cggcggaaga aaaggtctac aaacagcagc
      661 aaatcggtga gttcatgacc acgtggaagg attttcccag accagggctc gctgcgtggg
      721 cggagcgccg gaaggccggc ttgatgggca cggccaaaag cacccactac acgccgcgct
      781 ttcgcccctt cgacatgctc agcattccaa accagtcgtt ggtgattctc gaaaacgaat
      841 atcatcgcat cggtgccgag agcgtcgtcg gtgtgcagga cagctttcac cgctacgtcg
      901 actcggacat ggtctatttc caattttgcg gcggcagcaa agttgaaacc gagttcggcg
      961 tctacgagat ggcacccggc gatgtcatgc tggttcccgg gggcatcgcc catcgctcga
     1021 ttggacgcaa cgacagcctg cgctatttct gccagagcca cgaggcggtg gactacgtca
     1081 tgaacgagga ccagtacacg agtcagacca gcttcgaggt caagcggctt ggcggtccca
     1141 attggacggc ggcgcagggg acgaatgcgc cgggcaaggg gccggtgatt gagaagatgc
     1201 atttctggga cgatggcccg gaggaccaga cggtggtgga gcgtgattac gaatcgctcg
     1261 tcggcatcgc cggaatagga cgcggcaagc cgggaagtct cgtgcgcaag ctgcgcgctt
     1321 tcgatcactt cacggcgatc gtcggcaagg ggcgcgaaga cgccggcacc cagccgctga
     1381 tggaatcggc caatatgcgg atacgcacct acaatatgca ggacgagcag ttcgcctttc
     1441 atcgggcgct gcgcagcgag gaagtgcgga ttcagtttcg cggcgacgcg ctcgatttgt
     1501 ccgaatttga aaacgtcgaa gtttcgcccg gcgagatcac catcatcccg ctcggcattt
     1561 ctcacagcgt gatcagcatt ccgccggaag acgagaattt tctgcggctc aacttttata
     1621 gcaaagtccg ctggcgcgtc cccatcgatc cgaccaagca ttatttcgac agcaagttcg
     1681 agattgccac gacggtgcac aagcaggccg aatggcggaa gaaacttgcg gcggcacggt
     1741 gagtccagaa aatcgtgaag taaaagtctg acgccagata atattctcga ttttgaactt
     1801 gatagcaaaa tttgcggcac gccctagaat aactccattg ctagtagcga ctactgtgta
     1861 taaaagggat cgctagccta aaggagacgc cagatgaaaa tttatggagt tcgatggcgt
     1921 gatggttcgt tttctgtgac ccttgcgaaa aataagctgc atgctctcga agaatttgac
     1981 aagatcgccg acgcaaacgg gttgaagata gaagaatttg atgaattttt ggctacattc
     2041 aagctaaccg agagtggcga tattgtcctg gaagaaatag actcggatac tcgtggagaa
     2101 ctgggcagag tcgctagaaa ccgaaagcga ccaagccaat gcagcaaaaa aagaattggc
     2161 gcgcgacgtt agaattgcga aaaaaatccc aattgacccc gaagttaggc gaatcgctaa
     2221 gtcgcttcgc atgcaccccg accttgatga acggtataca cgatccaaaa ataagccgcg
     2281 aaagaaaaag tagtctcatg cccgaaccaa tggccacgca ggccgagtgg cgggagacac
     2341 ttgcgatggc gcggtgagga tgcggattag cccctatgct gtccatcccc cgcccttcgg
     2401 gagtaatgct tttcattcag agcactctga gatagtattt ccatatggca aaacgttccc
     2461 agcccgtgtc aggttcgcgc atttatccga tcattagcgg caaggagcgg ctagcgatct
     2521 gggagaaggc ccgtggtatg tggaaaaagc ggggaagcga tcccattcag gagcttaaaa
     2581 aaatgcgcgc gccctgggta acgaaacgct catttctcaa gtaagtgtac accctcgata
     2641 ccaacgcgat tctttactac ctcaaagatg ataatgatgc cgtggccatc ttgcgcggcg
     2701 tcttcgcgca aagttcgcca gtctacgtct ccgccgttac cgagctagag ctcttggcat
     2761 tcagtaacct caccagcgac gaggaattgc taatcgaaaa tctgctggcg accgttgcca
     2821 tcataccggt cgattcacac atcgctcgac tcgctgccct cgtgagaagg cggtatcgaa
     2881 tcaaagtgcc cgacagcatc atcgcagcca ccgcgatgtt taccggtagc atattgctca
     2941 cccgtaatac gcgcgacttc cgaaagatct cgaacctcgt aatacgaaag atctaagggc
     3001 aatactttgg ttcttagctg tatcgcagac aagtcacgca ggacttcaag gtgagacccc
     3061 gttgacactt ctaacatgac gatttccttc cggccgcgct ggtatttctt catcgcgctg
     3121 gtctccgtca ccttcatcgg gccgctctcc ctccacctct tcatacccgc catgccggcg
     3181 gtgaaggaag cgttcggggt ctcgaccggt atggcgcagc tcaccatgtc gctggcgatg
     3241 ttctcaatgg cctttttcac ggttgcctac ggcggcttgt ccgaccgctt cgggcgcaag
     3301 cgcgtgctcc tgggcgggct ggtgttgttt acctgcggcg ccgccagctg catggccgcc
     3361 gccaacatgc cgatgctgct ggcggggcgc atactgcagg gagcgggtgc gggctgtggc
     3421 gtggtgctgg cgcgcgccat cgcccgcgac gtctacggcc aggaccgcgt tgcccaggtg
     3481 atcgcctatc tcaccgccgc ctacgtgctc gggccgatgc ttgcgccgcc gatcggcggc
     3541 cacctgactg tgctgttcgg ctggcgcgcg ctgtt
//
LOCUS       DASQKS010000143         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3443117, whole genome shotgun sequence.
ACCESSION   DASQKS010000143 DASQKS010000000
VERSION     DASQKS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3443117"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(585..1766)
                     /locus_tag="VE170_03625"
     CDS             complement(585..1766)
                     /locus_tag="VE170_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14552.1"
                     /translation="MSTPQSHTLWTRTFVLLCTAQFFGSSQHALLQPTFPLYITSLGG
                     TPFQVGLVLACFAVTSVVFRPLIGGWADQWSEAGVSCCGLSLLSAAMLLCFIPFAEAI
                     MFANALRGLGWAAMSAAGYSLLALSAPPQRRGEASGYYSGVQASGTILFPAVALWLID
                     APFGGFHVVFTAALVLAALGAGTSGILFRRLPKKSHDRAGAELGPWWREILNVLDREI
                     ILASVLSFLSHVTFPALASFLVLYAREIGIDNLGWFFVVSGATSLLCRPLLGKVSDRI
                     GRGRALLACFALESVALAALAFSSSLFALMVSGMLYMVGLAMSSSTTLAIAMEQTKPE
                     RRGRAMATFSVALPLSNGIGALICGSIVQGLGFFWMYLAGAAFAAVGLIITIVNRAQL
                     K"
     gene            complement(1947..3128)
                     /locus_tag="VE170_03630"
     CDS             complement(1947..3128)
                     /locus_tag="VE170_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14553.1"
                     /translation="MTPANSDTLWTRAFVLLCIVQFLGYAQHFVLQPTLPIYVTHLGA
                     TPFVVGLVMASFAATSMILRPLVGYWSDRWSEAGVMISGLLIQGASVLLCYLPFVATT
                     MIANSCRGVGWASVNSGGYALLAQSAPAARRGEASGYYSGAQGSATILLPALALWLID
                     APFGGFHVVFAVSMSIAVTGAALGLLLRDRAVSSARQQRNALAGPWWREIIRIPDKDI
                     LLAAGLLFALHVSLPAVAGFLVLYARQLGIEHFGWYYVASGATSLIARPLLGQLSDKI
                     GRGAAIGAGFILEAAAFCVVVNATTLGGMIVSGMLYMTGSSMGTAATLALALQHANPE
                     RRGQVIATYSMAYPLSAGVGSLLAGSAIQIAGYSWMFIFIALLNIPGLWITWKNRASL
                     Q"
     gene            complement(3154..3369)
                     /locus_tag="VE170_03635"
     CDS             complement(3154..3369)
                     /locus_tag="VE170_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14554.1"
                     /translation="MKTANYFAASGFCRLRHRLYADQRLFFSLALSTFVDFYIAAPGS
                     LAHPTLIVGSECKISPPAAGSITAKFV"
     gene            complement(3438..>3698)
                     /locus_tag="VE170_03640"
     CDS             complement(3438..>3698)
                     /locus_tag="VE170_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ14555.1"
                     /translation="KALSRGAMLAAQCSACHGPKGDGDSARVIPALRGQPKGFLQTQM
                     ALFAGDKRKLEIAEMDEAKKRSFKDLTEADLADLATYYATLR"
BASE COUNT          864 a         1044 c         1159 g          631 t
ORIGIN      
        1 agcagaaacg gccgcgagat accccactcg cgggatcctc tttggctgct gtgcctggac
       61 cggaacgctt gcggtcaaga gtagagcaca aagacaagcg gccagagttt ttctactcat
      121 cggatctctc ctgagttttc ctgaccgcca acccatgaac tatttaagat ggccgattga
      181 gaaagcatgg caaaaacctg ccgcgtctcg gtgatcttgt aacgaaacaa cgaagtgatt
      241 agcgcaagag cattctcatg gggttaaact acttcttcct ttgattgggc cctaccgcta
      301 ggagcgacgc gggccacggc agatccggcc ggtaaacgcc ggccgccaac ctctgagaaa
      361 gtaataacga aagtgtttgc gagacgtcaa cgtgcagcgc gcaagtgttt tgattttcat
      421 aacttatttt agccgctgcc ggttgcgagg gtgattatca aagcgccgcg gcgggcgcgg
      481 gcaacgggca gatcctcagg ataacgcggg taggcaatcg ccgagaacga gagcgaaaat
      541 atttgtcaga catcatgcgc aagatcatgc cgacttgcct tagatcattt cagttgcgcc
      601 cggttgacga tggtgatgat caagccgacc gcagcgaagg ccgcgccggc gagatacatc
      661 cagaaaaagc ccaacccttg aacgatgctg ccgcagatca gcgcgccgat gccgttgctc
      721 aagggaagcg caacggaaaa cgttgccatc gccctgccgc ggcgttccgg cttggtttgt
      781 tccatggcga tcgccaatgt ggttgaactc gacatcgcca agccgaccat gtagagcatg
      841 cccgaaacca tcaaggcaaa aagactggat gagaaagcga gcgccgctag ggcgacgctc
      901 tccaaagcga agcacgcgag cagcgcgcgc ccgcggccga tacggtcgga aactttgccg
      961 agcagcgggc ggcacagtaa gctggtggcg ccgctgacga cgaagaacca acccaggttg
     1021 tcgatgccga tttcacgcgc atagagcacc agaaagctcg ccagcgccgg gaacgtcacg
     1081 tgggatagaa acgacaagac cgaggcgaga ataatctcac gatcgaggac attgagaatc
     1141 tcccgccacc aaggcccgag ttccgcgccg gcgcgatcgt gagatttctt gggcaggcgc
     1201 cggaatagaa tcccgctggt gccggcgccc aaggcggcaa gcacaagcgc ggccgtgaaa
     1261 accacatgaa agccgccaaa cggcgcgtca atcaaccaga gggccaccgc cggaaataaa
     1321 atcgtcccgc tcgcttggac gccgctgtaa taacccgacg cttcgccgcg ccgttgcggc
     1381 ggcgcgctga gagcgagcaa agagtatccc gccgcgctca tcgccgccca accgagcccg
     1441 cgcagcgcat tggcgaacat gatcgcctct gcgaagggaa taaagcagag caacatggca
     1501 gcggataaca acgacaagcc gcaacacgag acgccggcct ccgaccattg atccgcccat
     1561 ccgccgatca gcgggcggaa caccacactg gtcactgcaa agcatgccag cacgaggccg
     1621 acttgaaacg gcgtgccgcc gagggacgta atgtagagcg gaaaggtcgg ctgcagcagc
     1681 gcatgctgcg acgagccgaa gaactgcgcg gtgcaaagca agacaaaggt gcgcgtccag
     1741 agtgtatgtg attgtggcgt ggacatggat taggatagtt caccacgaag gcacgaagga
     1801 cacgaagatt tttggattca gacgttcctc ccgcaaaggc gccaagttcg gaggggaaat
     1861 agaaaaatta ctaagaaagt ttttcaccgt ttttttttct ccgaactttg cgagcgtggc
     1921 ggctttgcgg gagtttccgg tgacgatcat tgcaagctcg cgcggttttt ccaggtgatc
     1981 cacaggccgg ggatattgag cagggcgatg aaaataaaca tccagctgta tccggcaatt
     2041 tggatggcgc tgccggcgag caatgagccg acgccggcgc tgagcggata ggccatcgaa
     2101 taagtcgcga tcacttggcc gcgccgttcc gggttggcgt gctgcagggc gagcgccaac
     2161 gtcgccgccg tgcccattga cgagccggtc atgtaaagca ttcccgagac gatcatgccg
     2221 cccaaggtgg tggcgtttac gacgacgcag aatgccgcgg cttcaagaat aaaaccggcg
     2281 ccgatagcgg cgccacggcc aatcttgtcg gagagctggc cgagcagcgg gcgggcaatt
     2341 agactcgtcg cgccgctggc aacgtagtac cagccgaaat gctcgatgcc caactggcgc
     2401 gcgtagagaa ccagaaagcc ggcgacggcg ggcagcgaaa catgcaaggc gaacagcaag
     2461 ccggcggcga gcaaaatatc tttgtcggga attctaatga tctcacgcca ccagggaccg
     2521 gcgagggcgt tgcgctgttg ccgcgcgctg cttacggcgc gatcgcgcag gagcagtccg
     2581 agagcggcgc cggtgacggc gatactcatg gaaaccgcga agacgacgtg aaagccgccg
     2641 aagggcgcat cgatcaacca gagcgccaag gccggcaaaa gaatcgtcgc gctgccctgg
     2701 gcgccgctgt agtaacccga cgcttcaccg cggcgggcgg cgggagcgct ctgggcgagc
     2761 agcgcgtagc cgccggaatt gacgctggcc cagccgacgc cgcggcagct gttggcgatc
     2821 atggtggtgg cgacaaacgg caggtagcaa agcaacacgc tggcgccttg gattagcagg
     2881 ccggagatca tcacgccggc ttcgctccag cggtcggacc aatagcccac cagcggccgc
     2941 aagatcatgc tggtggcggc gaaggaagcc atcaccaagc caacgacaaa tggagtggca
     3001 ccgagatgag tcacgtagat tggcaaggtc ggttgcagca cgaagtgctg cgcgtagcct
     3061 aagaactgga cgatgcaaag cagcacgaaa gcgcgggtcc agagggtgtc ggagtttgcc
     3121 ggagtcatga agctgaacga gttccgacgt ttcttataca aacttcgccg tgatgctgcc
     3181 agccgcaggc ggcgatattt tgcattcgga gccgacgatg agtgttggat gagcaaggct
     3241 gccgggcgca gcaatataga agtcgacgaa ggtgctcagg gcgagcgaaa aaaaaagccg
     3301 ctgatccgcg taaagccgat gtcgcaatcg gcaaaatcca cttgctgcga aataatttgc
     3361 cgttttcact gcgccagcac cgttgccacg gggtttcggc ggggcaccgg ccggtgcgtt
     3421 cttcccgtgt tcgggaatta tcttagcgtc gcataatacg ttgccaggtc ggcgaggtcg
     3481 gcctcggtga gatctttgaa gctgcgcttt ttcgcttcgt ccatttcagc gatttccaat
     3541 ttgcgcttat cgccggcgaa cagcgccatc tgagtctgga gaaaaccctt gggttgtccg
     3601 cgtagggcgg ggatgacacg tgcgctgtcg ccgtcgcctt tggggccatg gcaggcgctg
     3661 cactgggcgg caagcatggc gccgcggctg agtgcttt
//
LOCUS       DASQKS010000144         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4698470, whole genome shotgun sequence.
ACCESSION   DASQKS010000144 DASQKS010000000
VERSION     DASQKS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4698470"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VE170_03645"
     CDS             complement(<1..252)
                     /locus_tag="VE170_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ14556.1"
                     /translation="MERKTANDFDPQLLSLFHKYIHGGINRRQFLDGAAKFAVGGMTA
                     MGLWETLRPNYALAQQVPKDDKRIKAEYTFYSSPKGNAAD"
     gene            complement(424..1347)
                     /locus_tag="VE170_03650"
     CDS             complement(424..1347)
                     /locus_tag="VE170_03650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ14557.1"
                     /translation="MERKNASDFDPQLLSLFHRYVHGGINRREFLDGAAKFAVGGLTA
                     MGLWEMLRPNYALGQQVPKDDKRINAEYIFFTSPNGNASDGKMRGLLARPASGNKFPV
                     VLVIHENRGLNPHIEDVARRLAILGFMALAPDALWPFGGYPRVDKYGNEADEQAAVLF
                     RKLDDGKYGEDCVAAAEFLLKHPQSTGKLGAVGFCNGGTKVNMLAVRMPQLAAAAPFY
                     GRAPAAADVPKIKAAVLAHFADLENDANTTGTWAAYEAALKANGIKHEGYIYPKTFHG
                     FHNDTTPRYDEAAAKLAWQRTIDHFNKTLKA"
     gene            1480..1917
                     /locus_tag="VE170_03655"
     CDS             1480..1917
                     /locus_tag="VE170_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14558.1"
                     /translation="MDDKRSIARTPEREALRRPLLDLHDALLRLHKVLLESERAVYEK
                     TVEPIRSPNHFFQLLTGDPWFAWLRPISQLIVAIDETLDDKEGLTNAGVDAVMRQAVF
                     LLIPAQNGGEFGERYVAALQRDPRVVLAHAQVAKRIGSGTTII"
     gene            complement(2010..3629)
                     /locus_tag="VE170_03660"
     CDS             complement(2010..3629)
                     /locus_tag="VE170_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14559.1"
                     /translation="MTGPNLAETVVDYLIAQNVRCIFGVLAHTLFPFGDAIAKRPELR
                     FINAQHEGGAGNMALGYARVTKQPAVCLVSAGGGATNIVTAVAQAYKEAVPLFVISSD
                     IGTASFAKGHWASWHGMEHVRLFQPITKQSARLGRVEELGSVLDSLFRQTTRGRQGPV
                     FFCIPEDLQEAHLPEPISFAISAHPAAPAVDANTVNTAVDLLLGARAPVMLAGTGVDW
                     AQAQDEVKELAELLALPVASSYTAKGVFPEKHPLALGCLGNGGRMYARPFFQQADLVL
                     AVGTSFSEGTTLGFGHKVMPARARIIHIDTDPEQFGRIYPAELAIQADAKNALRAIID
                     NAKARSPRPSNDRASAIQQAKQSWRKEIDAQIDTGNLTYASVLKALDDLLTGNEILTT
                     SGMTGDTLRNIDSKVPIIHAGEFRAIGTALATAFGVKIGRPDARVICVTGDGSFMMEQ
                     QELATARYHNIPVMVLILHNNAYGGMKRDQQNNYGGRIIGTELFIPDLAKLADLYGAK
                     GYTVTRKEQLTQVFKEALAADEFVIVDVQMD"
     gene            3872..4246
                     /locus_tag="VE170_03665"
     CDS             3872..4246
                     /locus_tag="VE170_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYJ14560.1"
                     /translation="MESTIQTFANVVIVQVKGRVDHSTAPEFGSVLLPQVEGCAKEDK
                     KLLVDLSKINYMSSAGLRVLMIAAKGCRKQEGHVVVAGMQPNVREVFKIGRFDMVMDS
                     YPTVRDALAAISPAAAAIYFER"
     gene            4250..4702
                     /locus_tag="VE170_03670"
     CDS             4250..4702
                     /locus_tag="VE170_03670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ14561.1"
                     /translation="MPHSTSCERTEALTIDAVAHCTEFASAQAWQAGFAAARVDEVEL
                     VVEEVVANICHYSYGDRLGNVELVCRVADGPRLELEFADAGQPFDMLAMPEPDLSVDI
                     DQRGLGGVGVPMMRALVDQANYRRDGERNLLRLVFHSNCRLAGPSQVA"
     gene            4706..>5404
                     /locus_tag="VE170_03675"
     CDS             4706..>5404
                     /locus_tag="VE170_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ14562.1"
                     /translation="MRICFWGTRGSLPVAIEAPAIRDKIKRALLTAAGRHFADEAALD
                     LFIDTELEFPVRAGYGGNTSCVEIVGGDHYTLCDMGSGLRRFGQRVLAQQGSARPQVY
                     NFFMSHVHWDHIMGFPFFPPAYIPGNTIRIHGCHSRALLEEAFLRQQSDPCFPVLWGR
                     LRATISFVELAADRWYEIDGLRVRAKLQPHPGDSYGYRFEKAGRSMIYSTDGEHKLKL
                     EEETAAMVEFYRDAD"
BASE COUNT         1050 a         1501 c         1645 g         1208 t
ORIGIN      
        1 atccgcggcg ttgcccttcg gtgacgagta aaaagtgtac tcagccttga tccgcttgtc
       61 gtctttgggg acctgctgcg ccaaagcata gttgggccga agcgtctccc ataatcccat
      121 cgcggtcatg ccgccgacag cgaatttcgc cgcgccatct aagaactggc gccggttaat
      181 gccgccatgg atatatttgt gaaataggct gagcagctgc ggatcgaaat cattagccgt
      241 ttttctttcc atgagtagct cctttaaatt tgattaattc gcgaggaggc tagcgcaaaa
      301 ccgatacttg gtcaattaca cattctaaag tgtgtagcgg tttggtgctt actgattacg
      361 cgccaagaaa atgcgacctt caaccttcgc tggtagaagt ttttgacgtg ctgctccatt
      421 ggtctaggct tttagcgtct tgttaaaatg atcgattgtg cgctgccaag cgagcttggc
      481 ggcggcttcg tcgtaacgcg gcgttgtgtc gttgtggaat ccgtgaaaag tcttcggata
      541 gatataccct tcatgcttaa tgccattggc ctttaaagct gcttcgtagg cggcccaagt
      601 gccggtagta ttggcgtcgt tctccagatc ggcaaaatgc gccagcaccg cagctttgat
      661 cttcggcacg tcggcagccg caggcgctct cccgtaaaat ggcgcggccg ccgcgagctg
      721 cggcatacgg accgccaaca tgtttacctt agtaccgcca ttgcaaaagc ccacggcgcc
      781 aagtttgccg gtcgactgtg gatgcttgag caagaattcc gccgcagcga cgcaatcttc
      841 gccgtatttt ccatcgtcga gctttctaaa cagtactgcc gcttgttcat cggcttcgtt
      901 gccgtacttg tccaccctcg gatagccccc gaacggccag agcgcatccg gcgccagcgc
      961 cataaagccc aagatggcca aacgccttgc gacatcttcg atatgtgggt taaggccgcg
     1021 gttctcgtga attaccagaa cgacaggaaa tttattgccc gacgcgggcc gggccagcaa
     1081 gccgcgcatc ttgccatccg atgcattgcc gttaggcgag gtaaaaaata tgtattctgc
     1141 attaatgcgc ttgtcgtctt tgggtacttg ctggcccagc gcatagttcg gccgaagcat
     1201 ctcccataat cccatcgccg tcaggccgcc gacggcgaat ttcgccgcgc catctaaaaa
     1261 ctcgcgccgg ttgatgccgc catgtacata tctgtgaaac agacttagca gttgaggatc
     1321 gaaatcgctg gcgttttttc tttccacggg tagctccttt ggtttggaaa aatcgcgtgg
     1381 aggctagcgt aaattgaccc ataggtcaat tccaattttg ccatccgtgg cactgccgag
     1441 ctagctgatt gcgcgccaag gaaatgatat ccttgatgca tggatgacaa gagatcgatc
     1501 gcgagaacgc cggaacgaga agcgctgcgc cggccgttgt tggacctgca tgacgcactg
     1561 ctgcgcttgc acaaggtctt gctggaatcg gagcgagcgg tttatgaaaa gactgtcgaa
     1621 ccgattcgct cgccgaatca tttctttcag cttcttaccg gcgacccctg gttcgcctgg
     1681 ctgcggccga tttcccagtt gattgtcgcc atcgatgaaa ccctcgatga caaggaaggg
     1741 ctgacgaatg ccggcgtcga cgcggtgatg cgacaggcgg ttttcctact gatcccggcg
     1801 cagaatggcg gcgagtttgg cgagcgctac gttgccgcgt tacaacggga tccgcgcgtc
     1861 gtcctcgccc atgcccaggt ggccaagcga atcggctcgg ggacgacaat aatctgaccg
     1921 cggcgcggct cgggacgatg ggcatcggcc cttgcggctc cgccgtgcct tctgcgcggc
     1981 ggcgaaagtt ttccccttct gaaaagcctt caatccattt gcacatcgac gatgacgaac
     2041 tcgtcggcgg ctagcgcttc tttgaaaact tgtgtcagct gctctttcct ggtcaccgtg
     2101 tagcctttgg cgccgtagag gtcggcgagc ttggctaggt ccggaatgaa cagttcggtg
     2161 ccgatgatgc ggccgccgta gttgttttgc tggtcgcgct tcatgccgcc gtaggcgttg
     2221 ttgtgcagga tcaggaccat caccggaatg ttatgatagc gcgcggtggc gagctcttgt
     2281 tgttccatca tgaacgagcc gtcgccggtg acgcaaatga cacgagcgtc ggggcggccg
     2341 atcttgacgc cgaaggcggt tgccagcgcg gtgccgatgg cgcgaaactc gccggcgtgg
     2401 atgatcggca ccttggagtc aatgttgcgc agggtatcgc cggtcatgcc cgaggtggtg
     2461 aggatttcat tgccggtgag cagatcgtcg agggctttca gcaccgaggc gtaggttaga
     2521 ttgcccgtgt cgatctgcgc gtcgatttct ttacgccagg attgcttggc ttgctggatc
     2581 gcgctggcac ggtcgttcga cggcctaggc gagcgcgctt tggcattgtc gataatggcg
     2641 cgcagggcgt tcttggcgtc ggcctggatg gcgagctcgg ctggatagat gcggccgaac
     2701 tgttccgggt cggtgtcgat gtgaatgatt cgcgctcgcg cgggcatgac cttgtggccg
     2761 aatccgaggg tcgtgccttc gctgaaactg gtgccgacgg cgagcaccag atcggcttgc
     2821 tgaaaaaatg gccgggcata catgcggccg ccgttgccga ggcagccgag ggcgaggggg
     2881 tgtttttccg gaaagacgcc tttggcggtg taactgctgg ccaccggcag cgctaacagt
     2941 tccgccagtt ctttgacttc gtcctgcgcc tgagcccaat cgacgccagt gccggcgagc
     3001 atgaccggcg cgcgggcgcc aagcagtaga tcaaccgccg tgttcactgt gtttgcatcc
     3061 accgcaggcg cggcgggatg cgccgagatg gcaaacgaaa tcggttcggg caagtgggct
     3121 tcctgcaaat cttcgggaat gcaaaaaaat accggtccct ggcggccgcg cgtagtctga
     3181 cgaaacaacg agtcaagcac cgaacctagt tcttcaacgc gtcccaggcg cgcgctctgt
     3241 ttggtgatcg gctgaaacaa acgcacatgt tccatgccat gccatgaggc ccaatggcct
     3301 ttggcgaacg atgcggtgcc gatgtccgac gagatgacaa aaagcggcac ggcttctttg
     3361 tatgcttggg cgaccgcggt gacgatatta gtggcgccgc cgccggccga gaccaagcat
     3421 accgcaggct gtttggtcac cctcgcgtag cccagcgcca tattccctgc gccgccctca
     3481 tgctgggcgt tgataaagcg tagttccggc cgcttggcga tggcgtcgcc aaaagggaag
     3541 agcgtatggg cgagtacgcc gaaaatgcag cgcacgtttt gcgcaatcaa ataatcgaca
     3601 acggtttcgg caaggttggg tcctgtcatg ggctgtctcc tttttggcac taggctcggc
     3661 ttatttcagc tcttatgaaa aagcaatggt cgtcgaaccc tgagccgctc cgatgatgcg
     3721 cgagctacgg caacgcggga ttattttacg ttcacggatc tgctccgtca tgttattatg
     3781 ggagcaaatt tatcgcgccg ccgagcagcg gagggagttt cccaacggcg cctaaagcga
     3841 tcccgaaaag ggatgcagac gaggtacgtg aatggaatcg acgatacaaa cttttgccaa
     3901 tgttgtaatc gttcaggtga aaggccgtgt cgaccatagc acggcgccgg agttcggcag
     3961 cgtgctgcta ccccaagtcg aaggttgcgc gaaagaagac aaaaagctgc tggtcgatct
     4021 cagcaagatc aattatatga gcagcgccgg cctgcgcgtc cttatgatcg cggcgaaggg
     4081 ttgccgcaag caggaaggcc acgtggtggt ggcgggcatg cagcctaacg ttcgcgaggt
     4141 tttcaagatc ggccgcttcg acatggtgat ggatagctat ccgacagtgc gcgatgccct
     4201 cgcggcgatt tcgccggcgg ctgcggcgat ctatttcgag aggtagggta tgccgcattc
     4261 aacttcatgc gagcgcaccg aggcgctgac catcgacgca gtggcgcact gcaccgagtt
     4321 cgccagcgcg caagcgtggc aggccggatt tgccgcggcg cgggtggatg aagtcgaatt
     4381 ggtggtcgaa gaagtggtgg cgaatatttg ccattacagt tacggcgatc gcctcggcaa
     4441 tgtcgaactt gtttgccggg tcgcggatgg tccgcggctg gagttggaat tcgccgatgc
     4501 cggacagccc ttcgatatgc tcgccatgcc cgagcccgac ctgtccgtcg acatcgatca
     4561 gcgcggtttg ggcggcgtcg gcgtgccgat gatgcgcgcg ctggtcgacc aggcgaacta
     4621 tcggcgcgat ggcgagcgca acttgcttcg cctcgtgttt cattccaatt gtcgattggc
     4681 cggcccgtcg caagtggcgt gacttatgcg catctgtttt tggggcaccc gaggttcgct
     4741 gcccgtggcc atcgaagcgc cggcgatccg cgacaaaatc aagcgggctc ttctgacagc
     4801 cgccggccgt cattttgccg acgaagcggc gctcgacctg ttcatcgata cagaattgga
     4861 attccccgtg cgcgccggct acggcggcaa cacctcatgc gtcgagatcg ttggcggcga
     4921 tcattacact ctttgcgata tgggctcggg cttgcgccgc ttcggccagc gagtgctggc
     4981 gcaacaagga tcggcccggc cgcaggtcta taatttcttc atgtctcacg tgcattggga
     5041 ccacatcatg ggcttcccat tttttccgcc ggcctacatt ccgggcaaca caatccgcat
     5101 ccatggctgc cattcgcggg cgttgttgga agaggcgttt ctgcgccagc aatccgatcc
     5161 ctgtttcccg gtgctctggg gtcgattgcg cgcgaccatc agttttgttg agctggcagc
     5221 ggatcgttgg tacgagatcg acggtctgcg cgtcagggcg aagctgcaac cccatcccgg
     5281 cgattcatac ggttatcgct tcgaaaaggc tggccggagc atgatctatt cgaccgacgg
     5341 agagcacaaa ctcaagttgg aagaggagac cgcggcgatg gtggaatttt accgcgacgc
     5401 cgat
//
LOCUS       DASQKS010000145         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_937374, whole genome shotgun sequence.
ACCESSION   DASQKS010000145 DASQKS010000000
VERSION     DASQKS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937374"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VE170_03680"
     CDS             complement(<1..1387)
                     /locus_tag="VE170_03680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYJ14563.1"
                     /translation="MPLSRKAGNRVPAKACRPSKFTKHLLNFDTLMFAPNALYRLARL
                     YNLQSSYFDGLGQPRQASTDAILQVLQALGAPLRRLDEAEDAWRQRRQSVWQQVIEPV
                     NVIWQNQLAINKIRVPTQLAEAAISYRIVLDSGATLAGELRDHPAAQPITRDVEGSRY
                     VTRRLVGLGQIPLGYHRLYLQIGRLEVESDLVCAPPRAYAPLDNRKRWGLFCPLYALH
                     SEHSWGAGDFSDLSRLVDFTASLGGHGCATLPMLASFLDEPFNPSPYAPVSRLFWNEF
                     YLDIERITELPDCAEAKSLLAAPDFIEDLKRLRAAPRVQYREVAARKREILTALLRTL
                     EKRHTQRSDDFATFIASRPVAQDYAEFRAKTERERKVWGQWPADNRHGKIHRDDFDEF
                     AKLYHLYVQWQCAEQIAEVGANARRKNTALYLDFPIGVNRDGYDVWRHQSLFALGING
                     GAPPDGLFVKGQ"
     gene            complement(1437..2492)
                     /gene="selD"
                     /locus_tag="VE170_03685"
     CDS             complement(1437..2492)
                     /gene="selD"
                     /locus_tag="VE170_03685"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2433..2435),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYJ14564.1"
                     /translation="MKLGKTKAVRLTQEVKSAGUAAKLGPGDLAQVLHDLPKFNDPNM
                     LVGTETADDAGVYRLRPDLAIVNTVDFFPPVVDDPYLFGQISATNSLSDVYAMGGDPK
                     TCMNIVCFPKGKLDISILGEILKGGAEKVKEAGAVIIGGHSIIDDEIKYGMAVTGVVH
                     PDKIFRNVGVQAGDVLILTKPLGTGIITTALKRGKASPESVQEAVASMTTLNAAASFV
                     VRKHKVHAVSDVTGFGVLGHAQEMASGSGVTLILDSAKLPLLRSAVRLAEQGYITGGC
                     KRNKDYLDDKMVIEKNVGEGLAEVALDPQTSGGLLIAVGKQHAAKLVDDLHAAGVTAA
                     CAIGYATSQQKPWVRLV"
     gene            complement(2526..>4398)
                     /gene="selB"
                     /locus_tag="VE170_03690"
     CDS             complement(2526..>4398)
                     /gene="selB"
                     /locus_tag="VE170_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HYJ14565.1"
                     /translation="KALTGTDTDRLKEEKERGISIDLGFAHLDLPDGTSAGVVDVPGH
                     ERFIKNMLAGAHGIDLVLFTVAADDGVMPQTEEHLDIVHLLGIKMAIFVITKIDLAPT
                     RIADVEEEIDILTLGTILENSPKISVSTVTGQGLSELKQLISTTLKSANHAAPSGYFR
                     LPVDRAFVLQGHGVVVTGTSLSGEIKVGEQVRCLPGDNLFRVRSLQVHGQSVERAGWG
                     QRVAMNLTGTERASIERGQVICDEKLALTSQRFDALLEVRPAAARGIKNHQRLRIHMG
                     AAERLGKIVLLGDKAKAEPKESVYCQITLDQPLLVLRGDRFVARDETAQRTLGGGAVI
                     HPWPARHKRGEANLLERLKALHSGDFGQLMETFLNDSSEFALAIDAIHQFLNLREADA
                     RQKVDALKFLRALNAEGERVYTTELKWNRLKERLLKVLKEFHVAHPLVPGMDMEELRG
                     KLGYQLSPKIFRGVIEQFLNEKLIAKEENLLRLISHKVQLGGQEKILMDKIKKILGEQ
                     PLAPPDLKEIEKQAGVPRNRLTEVIRLLERDGSVVRITTDMYFLASSIAQLRATLVQW
                     LSEKGEMNAAAFRDLIGSSRKYTIPLLEYFDRIGLTIRIGDIRKLKAPIAAAKTPAR"
BASE COUNT          843 a         1237 c         1348 g          970 t
ORIGIN      
        1 tttgtccctt gacgaacaat ccatcgggcg gcgcgccgcc attgatgccg agggcgaata
       61 gactctgatg gcgccagaca tcgtaaccat cgcggttaac gccgatggga aagtcgaggt
      121 agagcgcggt gtttttacgg cgcgcgtttg cgccgacttc agcgatttgc tcggcgcact
      181 gccattggac gtagagatga tagagcttgg caaattcgtc gaaatcgtcg cggtgaattt
      241 tcccatgacg attgtcggcg ggccactggc cccaaacttt ccgttcgcgc tcggtcttgg
      301 cgcgaaattc ggcgtagtct tgtgccaccg ggcggctcgc gatgaaagtt gcaaagtcgt
      361 cgctgcgttg cgtatggcgt ttctccagcg tgcgcaacaa ggccgttagg atctcgcgtt
      421 tgcgcgccgc cacctcgcga tattggaccc gcggcgccgc gcgcaatcgc ttcaggtctt
      481 ctatgaaatc gggcgccgcg aggagactct ttgcttcggc gcaatccggc agctccgtaa
      541 tgcgctcgat gtcgagatag aactcattcc aaaacagccg gctcaccgga gcgtagggac
      601 tcggattgaa cggttcgtct aaaaagctcg ccagcatggg cagagtggcg cagccatgac
      661 cgccgaggct cgcggtaaaa tcgaccagcc ggctgagatc ggaaaaatct cccgcgcccc
      721 agctgtgctc ggaatgcagg gcataaagcg gacagaagag tccccagcgc tttctattgt
      781 ccaatggcgc gtatgcccgg ggtggcgcgc agacgagatc gctctcgact tcgagtctgc
      841 cgatctgcaa atagagccga tggtagccga gcgggatttg ccccaggcca accaggcggc
      901 gcgtgacata gcgcgagcct tcgacgtcgc gcgtgatcgg ctgcgccgcg ggatggtcgc
      961 gcagttcgcc tgctaacgtc gcgccactgt caaggacaat tcgatagctg atcgcggctt
     1021 ccgccagctg tgtgggaacg cgaatcttgt ttatggccaa ttggttttgc caaatcacgt
     1081 tcactggttc gatgacctgc tgccaaactg attggcggcg ctgccgccag gcgtcttcgg
     1141 cttcgtcaag ccggcgcaac ggcgcgccga gagcctgcaa tacctggagg atcgcatccg
     1201 ttgacgcctg ccgtggttgc ccaaggccgt cgaagtaact ggactgaagg ttataaagcc
     1261 gtgcgagtcg gtagagggcg ttgggcgcaa acatgagtgt atcgaagttt agcagatgct
     1321 tggtgaattt actcggtcgg caagccttcg ccggcacacg gttaccggcc ttgcggctta
     1381 atggcacggg gttaacggct tcgcgttaat gacggatgta ggcacctccg caagtatcaa
     1441 accaagcgca cccagggctt ctgctgggag gtggcgtaac cgatagcaca ggcggcggtg
     1501 acgccggcgg cgtgcaggtc gtcaacaagt ttggcggcat gttgcttgcc gacggcaatg
     1561 agcaggccgc cggaggtttg cgggtcgagg gcgacttcgg ccaaaccttc gccgacattt
     1621 ttctcgatga ccattttgtc gtcgagataa tctttattgc gtttgcagcc gccggtaatg
     1681 tagccctgtt cggcgagccg caccgcgctc ctgagcagcg gcagtttggc cgaatcaaga
     1741 atcaaggtga cgccgctgcc actggccatc tcttgagcgt ggccgaggac gccgaagccg
     1801 gtgacatcgg aaaccgcgtg gaccttatgt ttgcgtacga cgaaggaggc ggcggcgttg
     1861 agggtggtca tcgaagccac cgcttcctgg acgctttccg gggacgcttt gccgcgcttg
     1921 agggcggtgg taatgatgcc ggtgccgagt ggcttggtca ggatcagcac gtcgcctgct
     1981 tgaacgccga cgttgcgaaa aatcttatcc gggtgaacca cgccggtgac cgccatgccg
     2041 tatttgatct cgtcgtcgat aatgctgtgg cccccgatga tcaccgcgcc cgcttctttg
     2101 accttttcgg cgccgccttt gaggatctcg ccgagaatgg agatgtccaa cttacccttg
     2161 ggaaagcaaa cgatgttcat gcaggtcttg ggatcgccgc ccatggcgta gacatcgctc
     2221 agagagttgg tcgcggaaat ctggccgaac aaataggggt cgtcgaccac cgggggaaaa
     2281 aagtccactg tgttgacgat cgccaggtcc ggacgcagac ggtacacccc ggcgtcgtcg
     2341 gcggtctccg tgccaaccag catgttggga tcgttgaact tcggtaagtc gtgtagtact
     2401 tgcgcaaggt cgcccggacc gagctttgca gctcaacccg cgcttttgac ctcttgcgtc
     2461 aagcgcacgg ctttagtttt tcctagtttc ataagcagaa actttagatc gaatgtcgcg
     2521 tcttttcaac gcgcgggagt ttttgccgcg gcgatgggag ctttaagctt gcgtatgtcg
     2581 ccgatgcgaa tcgtcagtcc aatccggtcg aagtattcca gcaacgggat cgtgtacttg
     2641 cggctggagc cgatcagatc gcgaaaagcc gcggcgttca tctcgccttt ttcgctcaac
     2701 cattgcacca gtgtcgcgcg caattgggcg atgctcgaag cgaggaaata catgtcggtg
     2761 gtgatgcgta ctaccgaacc gtcgcgttcg agcaggcgga tgacttcggt caagcggttg
     2821 cgcggcacgc cggcttgttt ttcgatttcc ttcaaatccg gcggcgccaa aggctgctca
     2881 ccgagaattt tcttgatctt gtccatcaag attttttctt ggccgccgag ctgaacttta
     2941 tggctgatta aacgcagaag attttcttcc ttggcgatga gtttctcgtt taaaaattgc
     3001 tcgataacgc cgcggaagat tttcggcgaa agttggtagc caagcttgcc gcgcagctcc
     3061 tccatgtcca tgccgggaac caaggggtgg gcgacatgaa attctttgag caccttgagc
     3121 agccgctcct tcagccggtt ccatttcagc tctgtcgtat agactctttc gccctcggca
     3181 ttgagtgcgc gcaggaattt caaagcgtcg actttttgcc gcgcgtccgc ttcccgcagg
     3241 ttcaagaact ggtgaatcgc gtcgatggcc agtgcgaatt cgctgctgtc gttcaaaaag
     3301 gtttccatta actgtccgaa gtcgccgctg tggagcgctt tgagccgttc cagcaggttt
     3361 gcttcgccgc gtttgtgccg cgcgggccag gggtggatca cggcgccgcc accgagggtg
     3421 cgttgggcgg tttcgtcgcg ggcgacgaag cgatcgccgc gcaaaaccag caacggttgg
     3481 tccagagtga tctggcaata caccgactcc ttcggctcgg ctttcgcttt gtcacccaac
     3541 aaaacaatct ttcccaagcg ttcggctgcg cccatgtgga tacgcaagcg ctgatggttt
     3601 ttgatgcccc tggcggccgc cggacggact tccagaaggg cgtcgaagcg ctgcgaggtg
     3661 agcgcgagtt tttcgtcgca gatgacctgg ccacgttcaa ttgatgcgcg ctccgtgccg
     3721 gttaaattca tcgccacccg ctgaccccaa ccggcgcgct ccaccgattg gccatgcact
     3781 tgcaggctgc gcacgcgaaa taggttgtcg ccgggcaggc agcgcacctg ctcgccgact
     3841 ttgatctcgc cggagagcga cgtgccagtg acgacgacgc catggccttg caagacaaaa
     3901 gcccggtcca ccggcagacg aaaatagccg gagggcgcgg cgtgattggc ggatttcaat
     3961 gtcgtggaga tcagctgctt taactcgctc aggccctgcc cggtcacggt tgaaaccgag
     4021 atcttgggcg agttttcgag aatggtgccg agggtaagga tatcgatctc ttcttcgaca
     4081 tcggcgatgc gcgtcggcgc cagatcgatt ttggtgatga cgaagatcgc catcttgatg
     4141 ccgagcaaat gaacgatgtc gagatgttct tcggtctgcg gcatgacgcc gtcatcggcc
     4201 gccacggtga atagaacgag atcgatgcca tgggcgccgg cgagcatgtt cttgatgaaa
     4261 cgctcgtgac ccggcacgtc gacgaccccc gccgaagttc catccggcag atcgagatgg
     4321 gcgaagccta agtcgatcga gatgccgcgc tctttttctt ccttgagccg atcggtatcg
     4381 gtgccggtga gtgccttg
//
LOCUS       DASQKS010000146         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3443191, whole genome shotgun sequence.
ACCESSION   DASQKS010000146 DASQKS010000000
VERSION     DASQKS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3443191"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VE170_03695"
     CDS             <1..521
                     /locus_tag="VE170_03695"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HYJ14566.1"
                     /translation="AAMIGWYGTAMLCYVTPKEHLGLPDKKDVKDGVIAYKIAAHAAD
                     LAKGHPGAQERDNALSLARFEFRWEDQFNLSLDPETAREFHDETLPAQGAKVAHFCSM
                     CGPHFCSMKITQDVRDYAAELGVQEEAALAAGMKEKAEEFKKSGGKIYQEVGAAAASE
                     NAAVTMRAAAKD"
     gene            595..1176
                     /locus_tag="VE170_03700"
     CDS             595..1176
                     /locus_tag="VE170_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14567.1"
                     /translation="MPRRSRKQPVRVLLYGFGPYRKFTENITAKIIGSLAPRPGLGKI
                     VFPVRFQRRQFIEALKFPMPVMVVGLGQSSRPRIEVETRAINRRRSSKTSALRPITAG
                     GEAWLPATMPLSVTRPSAKSINAGDYVCNFSMYVMLEEVERQDLDSLFGFVHIPLDHD
                     LKAACRYVERIVDNCFALADRLERGRAATRLGG"
     gene            complement(1165..2034)
                     /locus_tag="VE170_03705"
     CDS             complement(1165..2034)
                     /locus_tag="VE170_03705"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYJ14568.1"
                     /translation="MSEITLSGLTLFNRGKVRDIYDLGDQLLLVASDRISAFDVVLPT
                     PIPGKGKILTQLSEFWFGRFADIVPHHLITTNVDKFPGACQPHRAALEGRSMLVKKSS
                     PAPVECIVRGYLAGSGWKDYQQTGAVCGIPLPLGLVEAARLDEPIFTPSTKAAVGDHD
                     LNITFDAMIEKVGAVKAIKMRDATVAIYRRARALAETKGIIIADTKFEFGMEGDDILL
                     IDEVLTPDSSRFWPMDGYRPGKTPESFDKQFVRDYLLNLKWDMKPPAPELPDEIVRKT
                     QEKYTEALRRLTA"
     gene            complement(2099..2437)
                     /locus_tag="VE170_03710"
     CDS             complement(2099..2437)
                     /locus_tag="VE170_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYJ14569.1"
                     /translation="MSDLTLHPMKEIRIIIEGEQLKFATDLLDHKATGYTIIHNISGK
                     GHHGFHSAHPMFNEMDSVVMLMTVVAAENVEPILAGLKPLLARHTGVMFVSDVAVSRV
                     EYFSPDAERK"
     gene            complement(2487..>3243)
                     /locus_tag="VE170_03715"
     CDS             complement(2487..>3243)
                     /locus_tag="VE170_03715"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HYJ14570.1"
                     /translation="FIAGQHNTTTDEVELFDLEDLPPTHRKDVLQLLRDLARASARNN
                     QERCARFPEIHRTLRASKARREVRRRSSDWSQVRPEWGLSGNAAFIIGRRALTRGVHL
                     DGRVFLHSYDYRGDPDGKLLEAIMTGPQVVAQWINMEHYFSTVDQEVYGSGSKIYHNV
                     VGRLGVMTGPQSDLRTGLAWQTVMNGQQPYHEAMRLCTLVEAPRDLIDRIVRSQRPLQ
                     RLYDNQWLYLFAIDPQVQLCYRYIARLGWSASL"
BASE COUNT          656 a          917 c          982 g          688 t
ORIGIN      
        1 gcgccgcgat gatcggctgg tacggcacgg cgatgctctg ttacgtgacg ccgaaagagc
       61 atcttggatt gccggataag aaagacgtca aggacggcgt cattgcctac aagatcgccg
      121 cccacgccgc cgacttggcc aagggccatc cgggcgccca ggagcgcgac aatgccctca
      181 gcctggcgcg cttcgagttc cgctgggaag atcagttcaa tctcagtctc gatcccgaga
      241 cggcgcgtga gttccacgat gaaaccttgc cggcgcaggg tgccaaggtg gcgcatttct
      301 gctccatgtg cgggccgcat ttctgttcca tgaaaatcac ccaggacgtg cgcgattacg
      361 ccgcggagtt gggcgttcag gaagaagccg ctctggccgc cggcatgaaa gaaaaagcgg
      421 aagaattcaa aaaatccggc ggcaagattt accaggaagt gggtgcggcg gcagcgagcg
      481 aaaacgccgc tgtcaccatg cgggctgcgg ccaaagactg atcgctcccg acgctatgct
      541 catgtgaact ggccgcccgc ctggcggcca gttccatttt gacctcgttt atccatgcct
      601 cgtcgcagcc gtaaacaacc ggtccgggtg ctgctctacg gctttgggcc gtaccgcaag
      661 ttcacagaaa acattacggc aaaaatcatc ggatctttgg cgccccggcc ggggcttggc
      721 aaaattgttt ttccggtacg cttccagcgc cggcagttca ttgaagcgct aaaattcccc
      781 atgcctgtga tggtcgtggg cttgggtcaa agttcgcggc cgagaatcga ggtcgagacc
      841 cgcgcgatca atcgccgccg ctccagcaaa accagtgcgc tgcggccgat caccgcaggc
      901 ggagaggctt ggctgccggc gaccatgccg ctctccgtga cccgtccctc cgcgaaatca
      961 atcaacgccg gcgattatgt ctgcaacttc tcgatgtatg tgatgcttga ggaggttgaa
     1021 cggcaagatc tcgatagcct tttcggcttt gtgcacattc ccctcgatca cgacctaaag
     1081 gcggcgtgcc ggtatgtcga gcggatcgtg gacaactgtt tcgccctggc tgataggtta
     1141 gagcgtggcc gcgccgccac acggctaggc ggttaggcgc cgcagcgctt cggtgtattt
     1201 ctcttgagtc ttcctgacga tctcatccgg caattccgga gccggtggtt tcatatccca
     1261 tttgaggttg agcagataat cacggacaaa ctgtttgtcg aagctttcgg gcgtctttcc
     1321 cggtcggtag ccatccatcg gccagaagcg cgatgaatcg ggagtcaaca cttcgtcgat
     1381 cagcaaaata tcgtcacctt ccatgccaaa ctcgaattta gtatcggcga tgatgatgcc
     1441 cttggtctcc gccagcgcgc gggcgcgccg gtagatcgcg acggtggcgt cgcgcatctt
     1501 gatggctttg accgcgccga ctttttcaat catagcgtca aaggtaatgt taagatcgtg
     1561 gtcgccgacg gcggctttgg tcgacggcgt gaatatcggt tcgtcaagac gcgccgcttc
     1621 gaccaagcct aggggcagcg gtataccgca aaccgcgccg gtctgttgat aatccttcca
     1681 accggagccg gcgagataac cgcgcacgat gcattccacc ggcgccggcg agcttttttt
     1741 gaccagcatg ctgcggcctt cgagagccgc gcgatggggc tggcaggcgc cggggaattt
     1801 gtcgacatta gtcgtgatca gatgatgcgg tacgatgtcg gcaaaccggc caaaccaaaa
     1861 ttccgaaagc tgcgtgagaa tcttgccttt gcctggaatc ggcgtcggta agacgacatc
     1921 gaaagcggaa atgcggtcgc tggcgaccag cagcagttgg tcgccgagat cgtaaatatc
     1981 acggaccttg ccgcgattga aaagagtcaa accggaaaga gtgatttcgc tcatctgata
     2041 tcctttagcg gaaaagctgt ctgtgtcagt gcaacgacgt catcggccgc tggcgaggtt
     2101 acttgcgctc tgcgtcagga gaaaaatatt ccacccgact gacggcgacg tcggagacga
     2161 acatgacgcc agtgtggcgc gcgagcagcg gtttcagtcc tgccaaaatc ggctcgacgt
     2221 tttccgccgc caccacggtc atgagcatga cgacgctgtc catctcgttg aacattggat
     2281 gggcgctgtg gaagccatgg tggcccttgc cggaaatgtt gtggatgatg gtgtagccgg
     2341 tggccttatg gtcgagcagg tcggtggcaa atttgagctg ctcgccttcg atgatgatcc
     2401 tgatttcctt catcggatga agcgttaggt cgctcatggt cggtctcctt tcatgggcct
     2461 gttttcggaa tgacaaggga cagtatttat aggctggcgc tccatcccag tctggcaata
     2521 tagcgataac atagctgcac ttggggatcg atggcaaaga gatacagcca ttggttgtcg
     2581 tagagccgct gcaaggggcg ttggctgcgc acgattcggt cgatcaagtc gcgcggcgcc
     2641 tcgaccaagg tacacagccg catggcttcg tgatacggct gctgcccgtt catgacggtc
     2701 tgccatgcca gaccggtgcg cagatcgctc tgggggccgg tcatgactcc caggcggccg
     2761 accacgttat gataaatctt gctgccgctg ccgtagacct cttgatcgac agtggaaaag
     2821 taatgctcca tgttgatcca ttgggcgacc acttgcggcc cggtcatgat cgcctcgagc
     2881 agtttgccgt ccgggtcgcc acgataatcg taggaatgca aaaatacccg cccgtcgagg
     2941 tgaacgccgc gggtcaacgc gcgcctgccg atgatgaaag cggcgttgcc cgacagtccc
     3001 cactcgggcc ggacctggct ccaatcgctg cttcggcggc gcacttcgcg gcgcgctttc
     3061 gacgcgcgta gggtgcggtg aatttccgga aaacgcgcgc agcgttcctg attgttgcgc
     3121 gcgctggcgc gcgcaagatc gcgtaacaat tgcagcacgt ccttgcgatg agtcggcggc
     3181 agatcttcaa gatcgaagag ctccacctca tcggtggtgg tgttatgttg gccggcaatg
     3241 aag
//
LOCUS       DASQKS010000147        11401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1760105, whole genome shotgun sequence.
ACCESSION   DASQKS010000147 DASQKS010000000
VERSION     DASQKS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760105"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..355
                     /locus_tag="VE170_03720"
     CDS             62..355
                     /locus_tag="VE170_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14571.1"
                     /translation="MAASFQEAVVDMLVNPTIRAATANGAARIVLSGGVAANSRLRAK
                     MTEIAAGEGMQVFCPAPKFCTDNGAMIALTGYHWLQRGRRDAYDLNADADLTL"
     gene            390..1253
                     /gene="rsmA"
                     /locus_tag="VE170_03725"
     CDS             390..1253
                     /gene="rsmA"
                     /locus_tag="VE170_03725"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYJ14572.1"
                     /translation="MATLYEEVRALLRESEFRPRKSRGQNFLIHEHVIEAILRLLDLQ
                     PDDQVVEIGPGLGSLTRRLVERAQAVWAVEIDELLAQRLRAGEIGRNSKFNLIYGDIL
                     KVALPELLPDGKMKLAGNLPYNISTEVLFRIFDWREHFTSLVLMVQKEVADRIASGPG
                     ATAYGTLSVWCQVHGRVSEKINVSPEAFYPHPKVRSTILKIELLPQARVCEADLLVLQ
                     GLVRAAFGQRRKTLSNAVGSWLLRERKAIDDLLTAHDVDPKRRGETLTVDEFIKLAQA
                     IRHSGLLSASA"
     gene            1344..2165
                     /gene="mutM"
                     /locus_tag="VE170_03730"
     CDS             1344..2165
                     /gene="mutM"
                     /locus_tag="VE170_03730"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HYJ14573.1"
                     /translation="MPELPEVETVCRSLRPHLLGRTIERIDVREGRLRVPVDRQRVEC
                     LAGKRIEAVDRVAKYILLHLTGATVWLFHLGMSGKLICAASNIPSQKHDHIVVDLDDG
                     GQLRYHDPRRFGLAIVTHQEELSALSQLRRLGVDPFDEALNGDFLFRFTRASERRIRD
                     LLLDQQIVAGLGNIYANEVLAMARVKPTTRAWRLTRKQVAMIAAAIPELLHDAIQWCG
                     TSFSDYRDADDKSGEFQNHLRVYDRQGEPCRMCPGTIKRVAIGNRSAFYCPTCQK"
     gene            2419..3663
                     /locus_tag="VE170_03735"
     CDS             2419..3663
                     /locus_tag="VE170_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14574.1"
                     /translation="MSNSTATASKLLSPLRHRDFRLFWIGLLLSSVGSQFTTVAMAWQ
                     IYELTNSPLQIGLIGLARAVPQVGMLLFGGLLADAMDRRRLMIATQTSLFVVSGTLAF
                     LTISGSVTSLTLYGVTVFLALFSSLEAPSRHAIVTNLVPAEDLPRAMAISSTQRQIAT
                     IGGPSIAGLILAFSGPALCYAIDAVSWVIMLAVLLLIRAELPQRGGWRTVSFSALGVG
                     FRFVWGHPVIFPFLMMDLGVNIFGTVRSLYPIYARDILAAGPQGLGVLYAASAAGALI
                     GALGLSTFGQIRQAGRWILFGVAVYGLCLLGFAYSRWFWLSALLLLGSGVGDTISAIL
                     RSTINQLSTPDELRGRMSSINSIFTNSGPQLGQFQAGALAAVIGAEMAAGAGAVIILL
                     IVGVLVAGFAHVRDYQITRGDG"
     gene            4781..4933
                     /locus_tag="VE170_03740"
     CDS             4781..4933
                     /locus_tag="VE170_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14575.1"
                     /translation="MGDSSALFSPTDIEGIMAISLPMRFAAAIHQRSKRQNRPYVPVN
                     MGGDLP"
     gene            5030..5416
                     /locus_tag="VE170_03745"
     CDS             5030..5416
                     /locus_tag="VE170_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYJ14576.1"
                     /translation="MTNDHTFTGSADMNASFHNNLRQGLLSRRNKIAAILENLEFREV
                     EMRVRTLGHEDKLELDRLSSLGSLDDWYHRELIDLDNALARLDKGDFGVCLGCGAQID
                     PNWLEVFPEGEFCRNCEELRKWMELG"
     gene            5939..6340
                     /locus_tag="VE170_03750"
     CDS             5939..6340
                     /locus_tag="VE170_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14577.1"
                     /translation="MKRQTHKVLIVCSQVSAAVKTQLDCNGWRANWARDAKNAIAKVR
                     RESFDLAVLISTGPEMDVTETLFNLRELREALPIAVVQTSDLADTPPSAAFLAKDNNL
                     IAVRGLDNLARLLHLHGAAVVNQPSRDRPQP"
     gene            6388..6681
                     /locus_tag="VE170_03755"
     CDS             6388..6681
                     /locus_tag="VE170_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYJ14578.1"
                     /translation="MITRMILVKVPSDKSAEAERLWREDCGALLIEQPGCKSEQFLRS
                     RENPGEFISLSSWDNQEAIDRYRRSDAHRTIQQHTRALMNVAKVEVKTYEVAG"
     gene            complement(6725..7717)
                     /locus_tag="VE170_03760"
     CDS             complement(6725..7717)
                     /locus_tag="VE170_03760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ14579.1"
                     /translation="MKFSIHSLHGGVKNIDEIHKRAVRAEELGWDGIFLGESHLSSID
                     SFQTLASCAMLTKHVLLGIAVTNIVFRHPTVIAGAAASLNEISKGRAIMGLGTGDGPV
                     YTQGLKAMSMKDFEAGIKTIRNLVRGNAETFSTGKVGIGFKINQPAPIYVSAEGPKGL
                     QLAGRSADGVILGTGFDLRVYHWAKQKIREGAREAGRQEGEIKVIAAGMLCVDEDGNK
                     ARKIVRNRLANRAHHNFRFTCETVPPNELDGIKQFMAGFDVMKPMEDRVDPDLVNQYL
                     IQRFSIAGSPKECIERIEELKAAGVEHLMLTPARTVYSETVDAFAKNVIPHFRD"
     gene            complement(7815..8630)
                     /locus_tag="VE170_03765"
     CDS             complement(7815..8630)
                     /locus_tag="VE170_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HYJ14580.1"
                     /translation="MPIVFACAASHAPGMTAWTEAAPKEQSDNFLGSYRRLGALLVAA
                     KPDAIVALTVEHWANFFLNSMPTFCIGRAGHYDGPIEEWLRLPKARIPGDAKLSAELI
                     DACLDSGFDPLFSDELLLDHATFLPLHFLNPDMALPVVPVIINALTPPMPTAKRCFAF
                     GEFLGTVLQRSEKRIALIATGGLSHWPGEAQHGRINIPFDKKFLDNLVTGDHHQLTAY
                     THDEINSEAGSGGHEIRTWIALAGAVQSWKAELVAYEPVVPWATGCGLVRFSQ"
     gene            complement(8633..8887)
                     /locus_tag="VE170_03770"
     CDS             complement(8633..8887)
                     /locus_tag="VE170_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14581.1"
                     /translation="MSLYQLQKMIYHVNRDAERRGHYRQDPAAFSKKYDLTEQESAAV
                     LNVDVRALYVMGVHSLLLRPFTLLNKVSNEDYAKALKGLV"
     gene            complement(8894..9985)
                     /locus_tag="VE170_03775"
     CDS             complement(8894..9985)
                     /locus_tag="VE170_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ14582.1"
                     /translation="MSAPRTIEEVKNDIRDRVGHRAPFLHADKDESEQALARMPDFQG
                     ETWAAAWNALGAKWQDQALAAEKAGDAAAAKSAYLKSYGYYGIARHPFPSTPGKHHGY
                     LKTREMFLAASKYFDIPVEKVTIPFQGKAIVGHLRLPKQLPAPMIMHWGGIDNWKEER
                     HSFGENFVKQGWGCFIIDSPGTGECPLLAGPDAHQVHLAVLEYLLKRPEVDANKIAAV
                     GASFGGHWSTKLAHLAPDRLRAAVNWGGGIHYFFQPEWQERSRHADSYLFDLIEARAN
                     LFGKKTFDELCAVMPALSLKDSGLLDQPCAPLLIVNGKEDKQVPLEDFYLLLEHGQPK
                     TIRLFPGGHMGAIPNIFKTVVDWLHVKLD"
     gene            10217..11077
                     /gene="folD"
                     /locus_tag="VE170_03780"
     CDS             10217..11077
                     /gene="folD"
                     /locus_tag="VE170_03780"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HYJ14583.1"
                     /translation="MATIIDGKAVAKEVQQRIKEDVEGLERRWNMAPGLAVVLVGDDT
                     ASHIYVRNKEKACKEVGIKSFEHFLPATISERELLALVHQLNKDKHVHGILVQLPLPP
                     HIHSERILNAVSPYKDVDGFHPLNLGNLVVGADGYRPCTPLGVMKLLESVNCDPKGKN
                     AVIVGRSNIVGKPVALMLLEKHATVTICHSRTASLRDEVARGDIVVVAIGKPGIVRGE
                     WIKPGAVVIDVGINRLPNGKLCGDIDFESAKERASAITPVPGGVGPMTICMLLFNTLK
                     AAKDSLQRER"
     gene            11102..>11401
                     /locus_tag="VE170_03785"
     CDS             11102..>11401
                     /locus_tag="VE170_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14584.1"
                     /translation="MVMALKKTPLYAAHRRAGAKMVEFAGWEMPVQYRGVIEEHMAVR
                     AQAGLFDVSHMGEIEVRGAGALEFCQRLTANDVARLQVSQAQYSFLLNEQGGVVDD"
BASE COUNT         2344 a         3151 c         3381 g         2525 t
ORIGIN      
        1 cgcggcgtgg cactacctca aagagcaggg gccggaaaag tagcagccgc gccaggccga
       61 tatcgctgcc agtttccagg aagcggttgt cgatatgttg gtcaatccga ccatcagagc
      121 cgccaccgcc aacggcgcag cgcgcatcgt tttgtccggc ggggttgccg ccaatagccg
      181 gttgcgcgca aaaatgacgg aaatagccgc tggcgaaggc atgcaagtct tctgtcccgc
      241 gcccaagttt tgcaccgaca acggcgcgat gatcgcgctc accggctatc actggctgca
      301 gcgcggccgg cgcgacgcct acgacttaaa cgccgacgct gatctgacgc tgtaatacct
      361 ccagcatcga gatttcgaaa gtatcgagga tggcgaccct ctatgaagaa gtgcgcgccc
      421 tgctgcgcga gtccgaattt cgtccgcgca agagccgggg acagaacttt ctgattcatg
      481 aacatgtcat cgaagcgatc ttgcgcttgc ttgacttgca gcccgacgac caagtcgtcg
      541 agatcggacc ggggttggga tctctcacgc ggcgcttggt ggagcgggcg caagccgtgt
      601 gggcagtgga gatcgatgag ctcttggcgc aacgcttgcg cgccggcgaa atcggccgta
      661 actccaagtt taatttgatc tacggcgata ttctcaaggt ggcattgccg gagttattgc
      721 ccgatgggaa aatgaagctc gccggcaacc tgccgtacaa tatttccacc gaggtgctgt
      781 tccgcatctt cgattggcgc gagcatttca cttcgctggt attgatggtg caaaaagaag
      841 tcgccgaccg catcgccagc ggcccgggtg cgacggctta cggcacactg tcggtgtggt
      901 gccaggtgca tggccgagtc agcgaaaaaa taaacgtctc gccggaggcc ttttatccac
      961 atcccaaggt gcgttcgacg atcctaaaaa tcgagctgtt gccgcaggcg cgggtgtgcg
     1021 aagcggatct cctggtgctc caaggtctgg tgcgtgccgc cttcggccag cgccgcaaga
     1081 cactgagcaa tgccgtgggc tcttggctct tgcgtgaacg caaggcaatc gatgaccttt
     1141 taacggccca cgacgtcgat cccaaacggc gcggtgaaac cctcactgtc gacgagttta
     1201 tcaaactagc gcaagcgatc cggcatagcg gattgctgtc cgccagcgct tgaagtgcta
     1261 ccggttgtta cgcgcatgat agttgcagtc gcagtacata ttcatcccga ggtttcataa
     1321 ctcaatccga gcgactcccc gccatgcccg aactccccga agttgaaacc gtctgccgca
     1381 gtttgcgtcc ccatctcctt ggccgaacca tcgagcgcat cgatgtgcgt gagggccgcc
     1441 tgcgggtgcc ggtggataga caacgagtcg agtgcctggc cggcaaacgg atcgaggcgg
     1501 ttgaccgagt cgccaaatat attttactgc atctgaccgg cgccaccgtg tggctgtttc
     1561 atctcggcat gtcgggaaaa ctgatctgcg ccgccagcaa catcccgagc cagaagcacg
     1621 accatatcgt cgtcgattta gacgacggcg gccagctgcg ctatcatgac ccgcgccgat
     1681 tcggacttgc catcgtgact catcaagaag agcttagcgc actgtcacaa ctgcgccggc
     1741 tcggtgtgga tcccttcgat gaggcgctca acggtgactt tctgttcagg ttcacgcgcg
     1801 cctccgagcg gcgcattcgt gatctcttgc tcgatcagca gatcgtcgcc ggactcggca
     1861 atatttacgc gaacgaagtg ctggcgatgg ctcgggtcaa accgacgacg cgcgcatggc
     1921 ggctgacgcg caagcaggtg gcgatgatcg ccgcggcgat accggaatta ctccatgatg
     1981 cgattcaatg gtgcggcacg tcgttctccg attatcgcga cgccgacgat aagtctggcg
     2041 aatttcaaaa tcatctgcgg gtctacgacc gccagggcga accctgcagg atgtgcccgg
     2101 gcacgatcaa gcgagtcgcc atcggcaacc gcagtgcttt ttattgtccg acatgtcaaa
     2161 aatgacttgt cgctatttaa ttccgcgctt gggatgttcg aaatcgatcg ccatcagtgc
     2221 ccagaaaatc cgttaaaccg gttaacacca gtagccagag ctccattagc cacttgacca
     2281 cagatgcacc ggcatgaggg cgcagcacta acgcgctaac attccgtgtc ggataacgtc
     2341 acagctctaa atgcggcgcc ccttggaggg gtggagagca accggaggcg cactgccggt
     2401 ccgtaggatg acgaacgcat gagtaattcc actgcaactg cgtcaaaact cttatcgccg
     2461 ctgcgccatc gcgattttcg attgttttgg atcggcctgt tgctctccag cgttggcagc
     2521 caatttacca ccgtggcgat ggcctggcag atctacgagc tgaccaactc gccgttgcag
     2581 atcggcttga tcggtctggc gcgcgcggta ccgcaggtgg ggatgctgct gttcggcggc
     2641 ttgctcgccg acgccatgga tcggcgcagg ttgatgatcg cgacccaaac tagtttgttt
     2701 gtggtttccg gaactctcgc gtttctcacg atttccggga gcgttacctc cctgacgctc
     2761 tacggcgtta cagtttttct cgcgttattt agctcactcg aagcgccgtc gcgccatgcc
     2821 atcgtgacca atctcgtgcc ggccgaagat ttgccacgcg ccatggcgat ctccagcacg
     2881 cagcgccaga tcgccaccat cggcggtcca tcgatcgccg gtctgatcct agccttctcc
     2941 ggcccggcac tctgttacgc tatcgatgcg gtttcctggg ttatcatgct tgccgttctg
     3001 ctattgatcc gcgccgaatt gccgcaacgc ggcggctggc gcacggtttc gttcagcgcc
     3061 ctcggcgtcg gtttccgttt cgtctggggg catccggtga tttttccgtt cttgatgatg
     3121 gatctgggcg tgaatatttt tggcacggtg cgctcgctct atccgatcta cgcccgcgat
     3181 attctcgccg ctgggccgca agggttgggt gtgctctacg ccgccagcgc cgccggggcg
     3241 ctgatcggcg ctctcggtct cagtacgttc ggccagattc gccaggccgg ccgctggatt
     3301 cttttcggtg tggcggtgta cggactgtgt cttctcggct ttgcgtattc gcgctggttc
     3361 tggttgtcgg cgttactgct gttaggctcc ggcgtcggcg acaccatcag cgcgattctc
     3421 cgctcgacga tcaaccagct gagcacaccg gacgaactgc gcggccgcat gtcgtcgatc
     3481 aacagcatct tcaccaacag cggcccgcaa ctcggccaat tccaagccgg cgcgttagcc
     3541 gccgtcattg gcgccgaaat ggccgccggc gccggcgcgg tgattattct attgattgtc
     3601 ggagttctgg ttgccggttt tgcgcatgtg cgcgattatc agatcacccg tggtgatggg
     3661 tagtgaacgc tgcgccgcga cgtaagagta attttctttt tcagccagga acgcctgacg
     3721 gattgtgaga tttgtcgtgg catcgagatt tccggattgc ggattaatga tttgctcatt
     3781 caaaggataa tctcgtgtcg ttcttgctcg ctccggtact gtaatgattt tgctcatttc
     3841 atctgttgct cagcacttcg tcgatgattg cgccgtcgac aaattacccg cccagggcaa
     3901 acgagaattc gatgattttc gcattcttcg ttgatggctc gacgtgatcg ccgctagagt
     3961 aacccaagga acggttattg acggcacaac ggttgctttt tcccttgcga ggaggacgaa
     4021 aatggaaagc gaaagaggat cgttcggaat tgccatggac agcgcttttt caagttttct
     4081 gtaacatggc gccggccggt ttagggactg ccgcagcgga agtctccctg cggatttcgc
     4141 tggcggctgg tcgtcgatta cggcggcgac actgagcgtc ggggcgattg taaaagcttc
     4201 atcggtaaaa atattttcct attgacaact tggccggcga atcctagcgt tgcgttagat
     4261 ccatcgacaa gggatttcgg gggctgatgg atcacagaca gtaaacgctc ccgatgtcgg
     4321 aaaggaggtt cggagtatgg agagcgatac cccacctatc ccgcatcgcc cgctgtctgc
     4381 gggcggtcac ttcgccgaat caccaaggag ttgttccgat aggcctcaac tttaattgcc
     4441 tttaggcaaa atcgtcgctg cggcgacaaa ctgaggtagg tcgtagccgg cataccactc
     4501 cgaagatctt caaggtgagt gcggttcagt cgaagtaaca gcgaatcgca ggcatgcggg
     4561 gctcggaaaa taattgtgct ctaagcgtca aaagcgctac cgagggcatg tttccccggg
     4621 aagcatgccc tttcgtcttt cgagcatatt tcgcgtaaac ggaacggcga cgaacttcgt
     4681 gcgcctgccg gcttgaacgc gctccaatgg ctcgagcggc cgcccgacga agctgacgat
     4741 ggttgccgga ttacccggca agtcgcaagc ggacccagct atgggcgact catccgcctt
     4801 attctcgccg acggacatcg aaggtatcat ggcgatcagc ttgccgatgc gctttgcggc
     4861 ggcgattcac caacgcagca agcgccaaaa ccgtccttac gtaccggtaa acatgggggg
     4921 cgatctccca tgagcttttc ggcagccgac ccgactgtat tgggagtcga gaaccaagga
     4981 gacggctttt cggttggaaa gaagcgcact gtgtcgacgg atcgttatca tgaccaacga
     5041 tcatactttt acggggagcg ccgatatgaa cgcgagtttt cacaataacc tgcgtcaagg
     5101 actgctatcg cgtcggaata agattgccgc gatcctcgag aacctagaat tccgggaggt
     5161 ggagatgcgc gtaagaaccc tgggacatga agacaaactc gaactcgacc gattgagcag
     5221 tctcggttcc ctcgacgatt ggtaccatcg ggaactgatc gacttggaca acgcgttggc
     5281 gcgcctagac aaaggcgatt ttggcgtgtg cttaggttgc ggcgcgcaga tcgatccgaa
     5341 ttggctcgag gtctttcccg aaggagaatt ttgccgcaac tgcgaggaac tgagaaaatg
     5401 gatggagctt ggctgaggcg tctggcgaga gcggctattc gaggtaagct atggaatcgt
     5461 tgaccgcgcc tagggggaat agcggttcgg tattggcgat gtcggtgcag ggtcaggcta
     5521 ccgacgcctt cgcaatgccg gcatataaag aaatccggga atttcgttct gacgccaccc
     5581 gatggcacac gcgccaccga ttggatgatt ttcaatcgaa tctttatcaa gagccggaga
     5641 agatgttgat gttcgccatt cttgtcgacg caatttcttg ctttgacaaa ttgcccgccg
     5701 gggagggaat ggcgcgaaag agacaatggc tcgaggcaag gaactggctg tgcagtaacc
     5761 ggcaagactg gccgttcgca tttcgtaacg tgtgtgaagt tctaggtttc gacccgaatt
     5821 acttgcgccg cgggcttctg ggccggggcc aaacgcgacg tccggacgac cggcgcggcg
     5881 acacgcacgg tctcccgcgt aaaaaaacct atcattgccg gcactaaagg ttttccctgt
     5941 gaagcgccaa acccataaag tcttgatcgt ctgttcacag gtgagtgctg ccgtgaaaac
     6001 acagttggat tgcaatggtt ggcgagcgaa ttgggcgcgc gacgccaaga acgccatcgc
     6061 caaagtgcgc cgggagagct tcgatcttgc ggtgctcatt tccaccggac ccgaaatgga
     6121 tgtcacggaa acccttttca atctgcgtga gctgcgcgag gcgctgccca tcgcggtggt
     6181 tcagactagc gatcttgcgg atacaccgcc aagcgcggcc ttcctggcga aggataataa
     6241 tttgattgcg gtgcgaggat tagacaactt ggcgcgctta ctgcatttac atggagctgc
     6301 ggtggtaaat cagccgtcac gagatcgtcc gcagccctag gggaccgcga cgcaagtaac
     6361 cacactagcg agcaaggagg aacgtcgatg attacccgaa tgattctcgt taaggtgccg
     6421 tccgataagt ccgccgaggc cgaacggcta tggcgggaag actgcggcgc gctcttgatc
     6481 gaacagccgg gctgcaaaag cgagcaattc ttgcggagcc gggaaaaccc gggcgaattc
     6541 atatcgctta gcagctggga caaccaagaa gcgatcgatc gctatcgtcg cagcgacgcc
     6601 catcggacga tccaacaaca tacccgtgcg ttgatgaacg tcgcgaaagt agaggtaaaa
     6661 acctatgagg tcgccggata gcagtgacag atagcgaagt tcgagcccgg tcgctgggct
     6721 gcgatcaatc gcggaaatgc gggatgacgt ttttggcgaa agcgtcaacc gtttcagagt
     6781 agacggtgcg cgccggtgtg agcatcaaat gttcgacacc ggcggctttt agttcttcga
     6841 tgcgctcgat gcattccttt gggcttcccg cgatggagaa gcgctgaatc aaatactggt
     6901 tcactagatc tgggtcgact cggtcttcca tcggcttcat gacatcgaag ccagccataa
     6961 actgcttgat gccgtcgagt tcattcggtg gtacggtttc gcaggtgaag cgaaaattgt
     7021 ggtgggcgcg gttggccagg cggttgcgga cgatcttgcg cgctttgttg ccgtcttcgt
     7081 cgacgcagag catgccggcg gcgatgactt tgatttctcc ctcttgccgt cccgcttctc
     7141 tggcgccttc gcggatcttc tgtttcgccc aatgatatac gcgcagatcg aagccggtgc
     7201 cgaggatcac accgtcggcg ctgcggccgg cgagctgcaa gcccttgggg ccttcagcgg
     7261 aaacataaat cggcgccggc tggttgatct taaagccgat gccgacctta ccggtggaaa
     7321 aggtttcggc attgccgcgc accagattcc gaatggtctt gatgccggct tcaaaatctt
     7381 tcatcgacat ggctttcaag ccttgcgtat agaccgggcc atcgccggtg ccaagaccca
     7441 taatggcgcg gcctttggaa atctcattca gtgacgcggc cgcgccggcg atcacggtgg
     7501 ggtggcggaa cacgatgttg gtcacagcga tgccgagtag cacatgcttg gtgagcatgg
     7561 cgcagctcgc cagggtctga aaagagtcga tggaactcag atggctctcg ccgagaaaga
     7621 tgccgtccca ccccaactcc tcggcgcgca cggcgcgctt atggatttcg tcgatattct
     7681 taacgccgcc gtgcaggctg tgaatgctga atttcatgtc ggtcgctcct gggctttacc
     7741 gtaagctgcc caccatggcg gagccccaaa atggcgtaaa aaacgcgcag cttgctttgc
     7801 cgggctcgga tcattcattg ggagaatcgc accagaccgc atcccgtcgc ccagggcacg
     7861 accggttcat aggccaccag ttccgccttc cagctctgca ccgctccggc tagggcgatc
     7921 caggttctaa tttcgtggcc gccagagccg gcttcgctgt tgatttcgtc gtgggtgtag
     7981 gcggtcaatt gatggtggtc gccggtgacg agattgtcca agaacttttt gtcgaaaggt
     8041 atattgatgc ggccatgctg cgcttcgccg ggccagtggg ataggccgcc ggtggcgatc
     8101 agggcgatgc gtttttcgct tcgttgcaga accgtgccga ggaattcgcc gaaggcgaag
     8161 cagcgtttgg ccgtcggcat gggtggggtc aaagcattga tgatcaccgg caccacgggc
     8221 agcgccatgt cggggttcaa aaaatgcagc ggtaaaaatg tcgcatggtc caacaagagt
     8281 tcgtcggaga acagcggatc gaagccgcta tcgaggcagg cgtcgatcaa ttcggctgaa
     8341 agcttggcat cgccggggat gcgggctttg ggtagccgca accattcttc aatcggtccg
     8401 tcgtagtggc cggcgcggcc gatgcaaaag gtcggcatgc tattgagaaa gaaatttgcc
     8461 cagtgctcaa ccgtcagtgc gacgatggcg tccggtttgg ccgcgaccaa tagtgctccc
     8521 aagcggcggt agctgccgag aaaattatcc gactgttctt tgggcgcggc ttcggtccag
     8581 gcggtcattc ctggtgcgtg gctggcggcg caggcgaata cgatcggcat ggctagacca
     8641 atcctttcag cgctttggca tagtcttcgt tggagacttt gttgagcagg gtaaacggtc
     8701 gtaagagcag cgaatggacg cccatgacgt aaagagcgcg cacgtcgacg ttgagaaccg
     8761 cagcgctttc ttgctcggtc aggtcgtatt ttttgctgaa cgctgccggg tcttggcgat
     8821 aatgcccgcg ccgctcggca tcgcggttga cgtgatagat catcttttgt aattgatata
     8881 ggctcatgga gattcaatcc agtttcacat gcagccagtc gaccacggtt ttaaaaatat
     8941 tgggaatcgc ccccatatgg ccgccgggaa acaaacgaat cgtcttcggc tggccatgtt
     9001 cgagcaataa ataaaaatcc tccagcggaa cctgcttgtc ttctttgccg ttgacgatca
     9061 gcagcggcgc gcagggttgg tcgagcaggc cggaatcctt gagcgacaag gccggcatca
     9121 ccgcgcatag ctcgtcgaaa gtttttttgc caaatagatt ggcgcgggct tcgatcagat
     9181 cgaaaagata agagtcggcg tggcgcgagc gctcctgcca ttcgggctga aagaagtaat
     9241 gaatgccgcc gccccagttg accgcggcgc gcagtctgtc cggcgcgaga tgggcaagct
     9301 tggtcgacca gtggccgccg aagcttgcgc cgacggcggc gatcttgttg gcatcgactt
     9361 cgggccgttt gagcagatac tccaacacgg ccaaatgcac ctgatgggcg tccggtccgg
     9421 ccagcaaggg acattcgcca gtaccggggc tatcgatgat gaaacaaccc cagccttgct
     9481 tgacgaaatt ctcgccgaag gagtggcgct cttctttcca gttgtcgatg ccgccccagt
     9541 gcatgatcat cggcgccggc agttgtttcg gcagacgcag atgaccgacg atggctttgc
     9601 cctggaaggg aatcgttact ttttccaccg gaatatcgaa atatttcgac gccgcgagga
     9661 acatctcgcg ggttttcaaa taaccatgat gcttccctgg cgtgctggga aagggatggc
     9721 gcgcgatgcc gtagtaaccg taggatttta aataggccga cttcgctgcg gcagcatcgc
     9781 cggctttctc ggcggccaag gcttggtctt gccattttgc tccaagcgca ttccacgccg
     9841 ccgcccaagt ctcgccttgg aagtccggca tcctggccag ggcttgttcc gactcgtctt
     9901 tatcggcgtg gagaaacggc gcccggtggc cgacccgatc gcgaatatca ttttttactt
     9961 cttcaatggt tctcggtgcg ctcataaaac ttcctttgac cctatctaat gcgcaaacgc
    10021 ggtcgaggtc aatcgtcttt tcgctctaac gctgcgcagt cttctatcca ggcctctttg
    10081 tgtgaatatt tgtccggcaa aatcgtccat cttactgttg acctttctaa gtctccatgt
    10141 catcccaatg tttcagttag tctttactgg gcttttcatt tggatatagt ttccccaggg
    10201 atcgcggggt aaattcatgg cgactatcat tgacggcaaa gccgtcgcca aagaagttca
    10261 gcaacgaatc aaggaagatg ttgaaggctt ggagcggcgc tggaacatgg cgccggggct
    10321 ggcggtcgtg ttagtcggcg atgacacggc gtcgcatatt tatgtgcgca ataaagagaa
    10381 agcctgcaag gaagtcggca tcaaatcctt cgagcatttc ctgccggcga cgatttccga
    10441 gcgggaattg ctggcgctgg tgcaccaact gaacaaggac aaacatgttc acggcatcct
    10501 ggtgcaatta ccgctgccgc cgcatattca ctccgagaga attctcaatg cggtatcgcc
    10561 ttacaaagat gtcgacggtt tccatccgct caatctaggt aatttggtcg tcggcgccga
    10621 cggttaccgg ccctgtacgc cgctcggcgt gatgaaactt ctcgaatcgg tgaactgcga
    10681 tcccaaagga aaaaacgccg tgatcgtcgg ccgcagcaat atcgttggca agccggtggc
    10741 gctcatgttg ctcgagaaac atgcgacggt gacgatctgt cattcacgca ccgcgagttt
    10801 gcgcgacgaa gtcgcccgcg gtgatattgt cgtcgttgcc attggcaaac ccggcatcgt
    10861 gcgcggcgag tggatcaagc ccggcgcggt ggtgatcgac gtcggtatca accgtttgcc
    10921 gaacggcaag ctgtgcggcg atatcgattt cgaaagcgcc aaggagcgcg cctcggcgat
    10981 caccccggta ccgggtggcg tcggcccgat gacgatttgc atgttgctct tcaatacttt
    11041 aaaggccgcg aaagattcgc tccagcgtga acgctaaaac cattgatggg cgaccccggc
    11101 gatggttatg gcgttgaaaa aaactccgct ctacgccgcc catcgacgcg ccggcgccaa
    11161 gatggtcgag ttcgccggct gggaaatgcc ggtgcaatat cgcggcgtca tcgaagagca
    11221 tatggcggtg cgcgctcaag ccggcttgtt cgatgtcagc cacatgggcg agatcgaggt
    11281 gcgcggcgcc ggggcgctgg agttttgcca gcggctgaca gccaacgatg tggcgcgctt
    11341 gcaagtttcg caagcgcagt acagtttcct gctcaacgag cagggcggcg tggtcgacga
    11401 c
//
LOCUS       DASQKS010000148         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5541000, whole genome shotgun sequence.
ACCESSION   DASQKS010000148 DASQKS010000000
VERSION     DASQKS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5541000"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..702
                     /locus_tag="VE170_03790"
     CDS             307..702
                     /locus_tag="VE170_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14585.1"
                     /translation="MGKLRHLGIRTEDTGKLASFYVDVFDMKVIHKSKEEGGTIFLSD
                     GYFNLAIIPNKDQNSPNGLYHFGFEVENGEAIVDKMKKINPNKLPKPRPNGRPYAETR
                     GSDPDGNYFDISEHGFLDVQPVGEKKPGQ"
     gene            856..1746
                     /locus_tag="VE170_03795"
     CDS             856..1746
                     /locus_tag="VE170_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14586.1"
                     /translation="MAALWVARDRGLFRKHGIDMQFILMPRNPLAVAALLAGEIDAAI
                     VGPGHVINAAGSGADLIGIANLKQKLDYRLDARPEIKKPEDLRGKRIAISGPGSTSHL
                     QALLALQDFNIDANQAKITFMTIPGTEMNRRLALETGGIDATTLTGSVGDLYGNKGYT
                     VLYNSRNTGATLPQTMLVTTRRIAAAKPQVIEGYLKALIEAIAVTLDPANKEVVLRLL
                     ASNLRLTNTADAEESYQAVINSYERVPHVTVEGMKRLHKLMTQINSKLADVRVENAVD
                     NSFMTKLEAAGFIQSVYKKH"
     gene            complement(1890..2246)
                     /locus_tag="VE170_03800"
     CDS             complement(1890..2246)
                     /locus_tag="VE170_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14587.1"
                     /translation="MQTGKTRDPQFADTPTIYELMKEAKTAEPARQLLTMILAATDFG
                     RPIIAPPGVAADKTKILRAAFTKAMSDPELLADAKAQNLEITPSSGDDLEALAKQVMA
                     SQNPEVIERVKKLLGE"
     gene            complement(2337..3344)
                     /locus_tag="VE170_03805"
     CDS             complement(2337..3344)
                     /locus_tag="VE170_03805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ14588.1"
                     /translation="MIFDVEINSAAHYPAAEVPGLIEVAEQAGFGAFWKGESNSTDPF
                     VMLSSVASRTKKIKLGTAIYHIYGRSPVTLGIQAATLQDLSGGRLLLGLGVANKAIAG
                     WHGGVFDRPLKRAREYIEVVRKVAAGERVEYEGEIYNTGKRFQLSWKPSFPNVPIYLA
                     GLGPQMTKLVGKISDGVFINMATPAKVKEIAARVREGAIEAGRDPGQIEIIAKSRVSL
                     NPDKNAARAKLRQVLTFYNIADHYSDMLRGMGFDKEVSAIHEAFQKGGFKAAMGALTD
                     EYMDKLPVVPASDIGEIKEKMKAFEEAGVTRMVIPYVPVTEPVVDDARRFFEAWGSAR
                     A"
     gene            complement(3467..4441)
                     /locus_tag="VE170_03810"
     CDS             complement(3467..4441)
                     /locus_tag="VE170_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYJ14589.1"
                     /translation="MAALGYIEILDGKGGVSERIAVDGFPITVGRAYGNQVILADPFV
                     CPRHLKIVQDEQGRLLASDLDSINGFYAASDKERIAQLQLESGSQFRIGHTTLRYVSV
                     DHPLAPTLVEGAVKVLPLASPFAAVISGVILVLLLCLDAYLSSIERVTVANIIGETWV
                     TVSMLATWAGMWALASRIVVSRFNFARHVTIACLALLAISALGVVAEWSEFFLPWIPI
                     LWIAGVFGYGLVLAGMVFGHLGAASFLRRRSRLWTAAAISLVVVGTSAISDFAARSKF
                     STVMEYTGVVKPIDAALVPANSVEDFVTGSEKLKKELDALTKKAKALQ"
     gene            complement(4429..>5227)
                     /locus_tag="VE170_03815"
     CDS             complement(4429..>5227)
                     /locus_tag="VE170_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYJ14590.1"
                     /translation="HTLYPKVHFTGSLNPGMSGGPTLTQDGKVVGINVATEGNQISFL
                     VPAERAVALLDKTAKGEMRPSASFLADVGRQIHDNQSRYLAGMFEAKTPRVALAPYDL
                     PTKPAEFFRCWADAVRRKELPYVSVTHDCSTDDYIFVSSDQSSGIVRFYHQLLSTGEL
                     NPARFFTLYSAQLQGGNAAMFGNEEEVTSFRCQTRNVATAHGKLKGVLCARQYIKLPA
                     LYDAVFRVATLGSRNAGLVSTLSLSGVSFDNVQALSRRYMESIKWQR"
BASE COUNT         1133 a         1537 c         1478 g         1079 t
ORIGIN      
        1 aatgaaagag atttgcccaa ggggcggcta ctgccgcacc ttgcttgtgc tctttgtggt
       61 aagtgattcg agaatcctcg ccgtttcttg cagcgccacc tgttgcctaa tgtctgttgc
      121 ctcgtgtggc atctggccga agtcgcagcc gaagatgtaa agaacccggc tgatgtggtt
      181 gccacgatgg atcgcgccgt gggtttgctg tctttgcctt gacatgtcaa tgaccgatct
      241 tgtagcgttc acgcaatttc cacaggcgac cgaaatcaac gtttacgaaa tcacaaggag
      301 gggattatgg ggaaacttcg gcatctaggt attcgcacgg aagacacggg caagctggcg
      361 tcgttttatg tggacgtctt cgatatgaag gtgatccaca agagcaaaga agaaggcgga
      421 accatcttcc tctcggacgg ttattttaat ctggccatca ttcccaataa ggatcaaaac
      481 tcgcccaacg gcctgtacca cttcggcttc gaggtcgaaa acggcgaggc catcgttgat
      541 aagatgaaaa agatcaatcc caacaaattg cccaagccgc gccccaacgg cagaccctat
      601 gcggagaccc gcgggtcgga tccggacggc aactatttcg acatttcgga gcatggcttt
      661 ctcgatgtcc aaccggttgg cgagaagaaa cccggccaat aaacggcgat gaaaacttgt
      721 gcagctaaaa attccgtccc ggtcatccgg gtcggagttt tagttcttct cgcgctcatg
      781 ttcggtcatg cgctgccggt tcggttagcc gcccagacgc ggaccaactt ggccggcagt
      841 gtcactagcg aaagcatggc cgccctctgg gtggcgcggg accggggcct gtttaggaag
      901 cacgggatcg acatgcagtt tatcctcatg ccgcgcaacc ctttagccgt tgcggcgctg
      961 ctcgccgggg aaatcgacgc agcaatcgtc ggcccgggcc acgtcattaa cgccgcaggc
     1021 agcggcgcgg atctcatcgg catcgccaac ttgaagcaga agcttgacta tcggctggat
     1081 gccagacctg aaattaagaa gccggaagac ttgcgcggca agcgcatcgc catcagcggt
     1141 ccgggctcta cgtcgcatct gcaggcactc ctggctcttc aggacttcaa catcgatgcg
     1201 aaccaggcga agatcacctt tatgacgatt ccgggaacag agatgaatcg gcgtttggcg
     1261 cttgaaacgg gtggaatcga tgcaacgacg ttgacaggct cggtcggcga cctctacggc
     1321 aacaaaggat ataccgtgct ttacaactcc aggaacaccg gcgcgacatt gcctcaaacc
     1381 atgctggtga cgacacggcg catcgcggca gctaaacctc aagtcatcga aggttattta
     1441 aaagcgctga tcgaggcgat cgcggttacc ctcgatccag ccaacaagga agtagtcctg
     1501 cggctgctgg catcgaacct gcggctaacc aatacggcag atgcggaaga gtcctatcaa
     1561 gcagtcatta attcctacga acgggtaccc catgtcaccg ttgaaggtat gaagaggctg
     1621 cacaagctaa tgacccaaat caattctaaa cttgccgacg ttcgggtcga aaatgccgtt
     1681 gataattcct ttatgactaa gctagaagcc gcgggattca tccaaagcgt ttacaagaag
     1741 cattaacggc gccgtcagcc ccctcacctc tctcattaga gaaattcaag agggatatct
     1801 ctaaacccaa gagaacgggt ttcaccgcgt cgcaacaatc cggaaatctt atttacgttc
     1861 tttgcggcaa gtttcttttc cggaccatcc tactcgccta gcaacttctt cactcgctcg
     1921 ataacctccg gattctgtga cgccatcacc tgcttcgcca gcgcctccag gtcgtccccg
     1981 ctgctcggcg tgatttcgag attttgcgct ttcgcgtcgg cgaggagttc cgggtcgctc
     2041 atcgctttgg tgaatgcggc acggaggatc ttggtcttgt cagccgccac gcccggcggc
     2101 gcgatgatcg gccggccaaa atcggtcgcc gcgaggatca tggtcaataa ctgacgcgcg
     2161 ggctcggccg tcttggcttc tttcatgagt tcgtaaatcg tcggcgtatc ggcgaactgc
     2221 gggtcgcggg tcttgccggt ctgcaccagg acccgggcaa agccggtctt acgccagatg
     2281 aagtagggct cgcattgcgt cctaacgttg aaccttgaac gttgaactgc gcgcgcttaa
     2341 gcgcgcgcgc ttccccacgc ttcaaagaat ctgcgcgcat catcgaccac cggttcggtc
     2401 accggcacgt aggggatcac catgcgggtg acgccggctt cttcgaaggc tttcatcttt
     2461 tctttgatct cgccgatatc gctggccggc accaccggca gtttgtccat atactcatcg
     2521 gtgagcgcgc ccatggcggc tttgaagccg cctttttgaa aggcttcgtg gatcgcgctc
     2581 acttctttgt cgaagcccat gccgcgcagc atatcactgt aatgatcggc gatgttgtaa
     2641 aacgtcagca cctggcgcag cttggcgcgc gccgcgtttt tgtcgggatt gagcgaaacc
     2701 cgcgacttgg caataatctc gatctggccc ggatcgcggc cggcttcgat ggcgccttcg
     2761 cgcacccgcg cggcgatctc tttcactttg gccggcgtcg ccatattgat aaataccccg
     2821 tcggaaatct tgccgaccag cttggtcatc tgcgggccga ggccggcgag atagatcggc
     2881 acgttgggaa agctcggttt ccaggagagc tgaaagcgct tgccggtgtt gtagatctcg
     2941 ccttcatatt cgacccgctc gccggcggcg actttgcgca ccacctcaat atattcgcgc
     3001 gcgcgcttga gcggccggtc gaagaccccg ccatgccagc cggcgatcgc cttgttagcg
     3061 acgccgaggc cgagcagcaa gcgcccgccg gacaaatcct gcagcgtcgc cgcttggatg
     3121 cccagggtca ccgggctgcg gccgtaaata tggtaaattg ccgtgccgag ttttattttt
     3181 ttggtccggc tcgccaccga cgacagcatg acgaacggat cggtgctatt cgactcgcct
     3241 ttccagaacg cgccgaagcc ggcttgctcg gccacctcga tcagcccggg cacttcggcc
     3301 gccggatagt gcgccgcgga attgatctca acgtcaaaaa tcatgagtag cgtccttctt
     3361 tgggttgtaa ccttagacta ttgaaccctt gaaccttgaa cgaaattacc ggctagacta
     3421 gccagatggc ttcgcgcatg tcaaatcttc ataccttccg tcaggttcac tgcagcgctt
     3481 tggctttctt cgtgagggcg tcgagttctt tcttgagttt ctctgaaccc gtgacaaaat
     3541 cctccaccga attcgccggc accaaggccg cgtcgatggg cttgacgaca ccggtatatt
     3601 ccatgacggt ggagaatttg ctgcgcgcgg cgaaatcact gatcgcgctg gtgccgacga
     3661 cgaccagact aatcgccgcg gcggtccaca ggcgcgagcg gcgccgcaaa aaagacgccg
     3721 cgccgagatg tccaaaaacc attcccgcca gcaccagtcc gtagccgaat actccggcga
     3781 tccacagtat gggaatccat ggcaagaaaa actccgacca ctcggcgacc acgccgaggg
     3841 cgctgatcgc cagcagcgcc aaacaggcga tcgtgacatg ccgggcaaaa ttgaaacggc
     3901 tgacgacgat tcggctggcc agcgcccaca tgccggccca ggtcgccaac atcgacaccg
     3961 tgacccaagt ttcgccgatg atatttgcca cggtcacccg ctctatactg gaaagatagg
     4021 catccaaaca gagcagcaac accaagatca cgcccgaaat caccgccgca aagggcgacg
     4081 ccaggggcaa aacttttact gcgccctcga ccaacgtcgg cgccagcggg tgatcgacgc
     4141 tgacgtagcg cagggtagtg tgaccgatac ggaactggct gccggattca agttgcaact
     4201 gggcgatgcg ctctttgtca gaggcggcat agaaaccgtt gatgctatcg agatcgctgg
     4261 cgagcaaacg cccttgttcg tcttggacaa tcttgagatg acgcggacag acgaacggat
     4321 cggcgagaat cacctgattg ccataggcac ggccgacggt gattggaaat ccatccacgg
     4381 caatccgctc gctgacgccg cccttgccgt cgagaatctc gatatagcct agcgctgcca
     4441 cttgatgctc tccatgtagc gccggctcaa cgcctgaaca ttgtcgaagc tcaccccgga
     4501 gagcgaaagc gtcgaaacca agccggcatt gcgcgagccg agggtggcga cgcgaaacac
     4561 cgcgtcgtag agcgccggca acttgatgta ctggcgcgcg cacagcactc ccttgagctt
     4621 gccgtgggcg gtggcaacgt tgcgcgtctg gcagcgaaac gacgtgactt cctcttcgtt
     4681 gccgaacatc gccgcgttgc cgccttgaag ctgtgcggag taaagcgtga agaagcgcgc
     4741 cggattgagc tcgccggtgg acaacagttg atgataaaaa cgcacgatgc cggaggactg
     4801 gtcgctggaa acgaaaatgt aatcgtccgt cgaacagtca tgggtcaccg aaacgtaagg
     4861 caattcctta cggcgtacgg cgtccgccca acagcgaaaa aactcggcgg gtttggtcgg
     4921 caagtcgtac ggcgccagcg ccacccgcgg cgttttggcc tcgaacatgc cggcgagata
     4981 gcgcgattga ttgtcgtgga tctgccggcc gacatcggcg aggaaactcg ccgacggccg
     5041 catttcgccc ttggcggtct tatccagcaa agccacggcc cgctccgccg ggacgagaaa
     5101 gctgatttga ttaccttcgg tggcgacgtt gatgcccacg actttgccgt cttgcgtcaa
     5161 cgtcggcccg ccgctcatgc cgggattgag cgagccggtg aaatgcactt tgggatagag
     5221 cgtatgt
//
LOCUS       DASQKS010000149         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5118711, whole genome shotgun sequence.
ACCESSION   DASQKS010000149 DASQKS010000000
VERSION     DASQKS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5118711"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VE170_03820"
     CDS             complement(<1..1074)
                     /locus_tag="VE170_03820"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYJ14591.1"
                     /translation="MQLTLAVHPIADIQFGNRLSLEGSALTVDGEELRSLVLEDAAID
                     AVAFDIARPGESCRAGPIFDVVEPRAKADGGSDFPGIIGAPTTAGIGTTHVLAGAAVS
                     VLAEMSPDPSRSPTGRVLEMSGSAAAGSAYSKLCHLLVLPQTKAGLPRSAVLKAYRVA
                     SIKVAVHLARAALAQAPAATQTFSPIGAEDKSREELSRVVYIGQIFSRQRKPAVDEPI
                     LYGTNTDGMLPVILHPDEWLDGALVPSLNSWFGGTETYFYQNHPVILDLYRRHQAREI
                     NFVGTIATVAGNDNVDRVRQCHAAANLAKWNLGADGAVMTKYGGGLPHADLSETARLL
                     EHMGIKTAVMVSDVARDRRVESAL"
     gene            1225..>2350
                     /locus_tag="VE170_03825"
     CDS             1225..>2350
                     /locus_tag="VE170_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HYJ14592.1"
                     /translation="MKSAKSGAELPGSFNTMLRFSPLPNRADLIPWQEWSGTAFRAAQ
                     AEDKPVMLYLSAFWCRFCQRMDEQAFSETENIALLNAYFIPLRVEYMQRPDVDARYNL
                     NGWPTIAFMTPAGQLLAAVNYLPTDQFKELLIDVYMSYQQRKDALRAAAQDAGESQAP
                     VRQFADSELAINLAKITESVMALADRTHGGYDRGQKFIHPQVNDFLLRRYEATRDRQY
                     LDQVCLTLERMRAGELYDHRGGAYFRTSSGADWSHPHREKLLLEEAGLLANCLSVFRI
                     TQRAEYRRMAGEIIEYLDTKLFDPASGTFFGCEDWLRYEPPADDGEEFFTIIDRCVYT
                     DANAVASAAYLDAAALLDQPAYRERGLAALEFLWRNCRGDG"
BASE COUNT          453 a          692 c          730 g          475 t
ORIGIN      
        1 gagcgccgat tcgacgcgcc ggtcgcgcgc cacgtcggaa accatcaccg cggttttgat
       61 gcccatatgt tccaacaacc gcgcggtctc ggacaggtcg gcgtggggca agccgccgcc
      121 gtatttggtc atcacggcgc cgtctgcgcc cagattccac ttggctaaat tcgccgcggc
      181 atgacattgc cttacgcggt cgacgttgtc gttgccggcg acggtggcaa tggtgccgac
      241 aaagtttatc tcgcgcgcct ggtggcgccg gtagagatcg agaatcaccg ggtgattctg
      301 ataaaaatag gtctccgtcc cgccgaacca tgaattgagc gacggcacca gcgcgccgtc
      361 gagccattcg tcgggatgta aaataaccgg cagcatgccg tcggtattcg ttccataaag
      421 aatcggctcg tcgaccgccg gtttgcgctg gcgcgaaaaa atctgcccga tataaacgac
      481 gcgggacagc tcttcacggc ttttgtcttc ggcgccgatg ggcgaaaatg tctgtgtcgc
      541 cgcaggtgct tgcgccagcg cggcgcgggc aagatgcacg gcgactttga tgctggcgac
      601 acggtaggct tttaacaccg ccgagcgcgg caggcccgct ttggtctgcg gcagcaccaa
      661 caggtggcag agcttcgaat aggcgctgcc ggcggcggca ctgccgctca tctccaacac
      721 ccggccggtg ggactgcgtg aagggtcggg cgacatttcg gcgaggaccg agaccgccgc
      781 gccggcaagc acatgagtcg tgccgattcc cgcggtggtc ggcgcgccga tgatgccggg
      841 gaaatcgctg ccgccgtccg ccttggcgcg gggctcgacg acatcgaaga tcggtccggc
      901 gcggcagctc tcgccgggac gggcgatatc gaaagctacg gcatcgatgg cggcatcttc
      961 caacactagg ctgcgcaatt cctcgccatc gaccgtaagc gcgctgccct caagggataa
     1021 tctattgcca aattgaatat cggcgattgg atggacggca agggtgagct gcatgcaacg
     1081 atctcccgat tgacagatca aacttcgact ccagacaatc tatgtcccgg cgcttgcgct
     1141 tgtcaactta taggcctgga tagcgaatcc ggcgggcgat tattttcggc agacaaatcc
     1201 gctttgtggc ctttcttcgc ggccgtgaaa agtgctaaaa gcggggcaga attgccgggt
     1261 tcgttcaata ccatgttacg cttctcgcca cttcccaacc gcgccgatct tattccttgg
     1321 caggaatgga gcggcacggc attccgcgcg gctcaagccg aggacaagcc ggtgatgctg
     1381 tacttatcgg cgttctggtg ccgattctgt cagcgcatgg atgagcaagc tttttccgag
     1441 actgaaaaca tcgcgcttct gaatgcttac tttatcccgc tgcgggtcga gtatatgcag
     1501 cggcccgacg tcgatgcgcg ttacaacctg aacggctggc cgaccatcgc gtttatgaca
     1561 cccgctgggc agttgctcgc tgccgtcaat tatttgccga cggatcagtt caaagaattg
     1621 ctcatcgacg tgtatatgag ctaccagcaa cgcaaagacg cgcttcgtgc cgcggcgcaa
     1681 gacgccgggg agtcgcaggc tccagtgcga cagtttgccg attcggagtt ggcgattaac
     1741 ctggcgaaga tcaccgagag cgtgatggcg ctggcggacc ggacccacgg cggctacgat
     1801 cgcggccaga agttcatcca tccgcaggtc aatgactttc tgctccgacg gtacgaagcg
     1861 actcgagacc ggcaatatct cgaccaagtc tgtctgactc ttgaacgcat gcgcgccggc
     1921 gagctctacg atcatcgagg cggcgcttac ttccggacgt cgtccggcgc ggattggagc
     1981 catccgcatc gggaaaaact tttattggag gaggccgggc tgctggccaa ttgcttgtcg
     2041 gtttttcgca tcactcagcg ggcggaatac cggcgcatgg ccggggagat catcgagtac
     2101 ttggatacta agcttttcga cccggcgagc gggacgttct tcggctgcga ggattggttg
     2161 cgctatgaac cgccggccga tgatggcgag gaattcttca cgatcatcga tcgctgcgtc
     2221 tataccgacg ccaacgcggt tgccagcgcg gcgtatctcg atgccgcggc gctattggac
     2281 cagccggctt accgcgagcg agggctggcc gcgctggaat ttctctggcg aaactgccgc
     2341 ggcgacggcg
//
LOCUS       DASQKS010000150         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4701702, whole genome shotgun sequence.
ACCESSION   DASQKS010000150 DASQKS010000000
VERSION     DASQKS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4701702"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /locus_tag="VE170_03830"
     CDS             <1..1859
                     /locus_tag="VE170_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ14593.1"
                     /translation="AVQSTATMLHPNLQSYRGLLHEITAPSNVFVDLTWTKGDAEAGF
                     RDADLIVENTFTTQPMHQAYIEPHACVVRAHSAGRADVWACSKVPFALREQVATALRR
                     PVGDFLMHPSYIGGCFGGKGDFMDVPVCYLLSLQSGRPVKMVMDYAEEFIAGNPRHGA
                     VIKVKTGVNKDGRLIAHRMDYVYDSGAYGAFKPQGYLVGPKEAAGPYKIPNVHINEKI
                     VYTNKIPCGHMRAPGEPQGFFANESQMDLVARRLAMDPIAFRRKNLMHDGDISPIGHA
                     LPHIKSDETLALALTTSGYLKPKRKYVGRGLALAQWLPLGGEGHAFVSVDIGGAVTVA
                     TAMVDQGAGTYTAMRQIVANELQVPVAQVGCQILDSGAAAADTGVGASRATRIFGNAT
                     HAAAIQVKAKLLQGASEILGVPTDELFFTGGGSIRAHSGKQVSYAAIVERLGGEVRCE
                     GSYNNFTNGPAAALVVQVAEVEVDPETGQVVIENFTTAHSTGTIINPLMHQGQIDGGV
                     VTAIGYALMEQLVIEQGKVITTNFGENKIPSIRDIPPLKTVIQEFPVGNGPYGGMSIG
                     EPPVLVAAAAIANAVHDAVGVRVYDLPITAEKVLTALKESSDDLTTKGTRIT"
     gene            2010..2516
                     /locus_tag="VE170_03835"
     CDS             2010..2516
                     /locus_tag="VE170_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14594.1"
                     /translation="MKDELAKRLVQSLKDAGINFVTYLPETRLSQIVPLIRDDPSMEL
                     IAVANESEAITIAAGATLGGKQAAVYMENTGIYTSCYALLTVGKQLGVPLLLVIAYLG
                     GVPDWRNSFLYATIGKHSIPVLDGLGVDHTLIEDGVKLEIKITDAVRAANALRAPYAL
                     LFAGEFSE"
     gene            2513..>2809
                     /locus_tag="VE170_03840"
     CDS             2513..>2809
                     /locus_tag="VE170_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ14595.1"
                     /translation="MIRYECLQLLAGKMTDQLVVTSQSGQRIEWSHLSRHEGNLLVGM
                     MGCAIGVATGLALALPQRKVIALDSDGSVLLSLFNLATLGNLQPKNLVVYVFDNG"
BASE COUNT          607 a          799 c          848 g          555 t
ORIGIN      
        1 aagcggtgca atcgaccgcg accatgctgc atcccaacct gcaaagctac cgaggcttgc
       61 tgcacgaaat cacggcgccg agtaatgtgt tcgttgatct gacctggacc aagggtgatg
      121 ccgaagcggg attccgcgac gccgatttga tcgttgaaaa taccttcacg acccaaccga
      181 tgcaccaggc ttacatcgag ccacatgctt gcgtcgtgcg ggcgcatagc gccggccgcg
      241 ccgatgtctg ggcctgttcg aaagtgccgt ttgctctgcg cgagcaagtc gctacggcgt
      301 tacgccggcc ggtgggcgat tttctcatgc accccagtta catcggcggc tgcttcggcg
      361 gcaagggcga tttcatggac gtgccggtct gttatttact gtcgctccag agcggccggc
      421 cggtgaagat ggtgatggat tatgcggagg aattcatcgc cggcaaccca cgccatggcg
      481 cggtaatcaa agtgaagacc ggcgtgaaca aagatggccg gctgatcgcg caccgcatgg
      541 actatgtcta cgacagcggc gcctatggcg cgttcaaacc ccagggttat ttagtcggtc
      601 ccaaggaagc ggccgggccg tacaagatcc ccaacgtcca tatcaacgag aaaatcgttt
      661 acaccaataa aattccctgc ggccatatgc gcgcgccggg agagccgcag ggcttttttg
      721 ccaacgaatc gcaaatggac ctggtggcgc gccggctggc catggatccg atcgcatttc
      781 gacggaaaaa tctcatgcac gacggcgata tttcgcccat cggtcacgcg ctgccgcata
      841 tcaaaagcga cgagaccctc gctctagctt tgacgacgtc aggatacctc aagccaaagc
      901 gcaaatacgt cggccgcggc ttggcattgg cgcaatggct gccgttgggc ggtgagggac
      961 atgcgttcgt gtcggttgat atcggcggtg cagtcacggt ggcgaccgcc atggtcgatc
     1021 agggagcggg cacctacacg gcgatgcggc agatcgtcgc caatgagctg caagtgccgg
     1081 tagcgcaagt tgggtgtcaa attctcgact ccggtgccgc cgctgcggac accggtgtcg
     1141 gcgcgagcag ggcgacgcgg attttcggta atgccaccca tgccgcagca atccaagtca
     1201 aagcaaagtt gttgcagggc gcgagcgaaa tcctcggagt gccgacggat gagctttttt
     1261 tcaccggcgg cggatcgatt cgcgcccaca gcggcaaaca ggtttcttac gccgcgattg
     1321 tcgaacggct cggtggcgag gttcgctgcg aaggctcgta caacaatttc accaacggtc
     1381 ccgcagccgc gctggtcgtg caagtggccg aagtcgaggt cgatcccgag accggccagg
     1441 tcgtgatcga gaatttcacc accgcgcata gcaccggcac catcatcaat ccgctgatgc
     1501 accaagggca gatcgatggc ggggtcgtaa ccgcaattgg ttatgcgctg atggagcagc
     1561 tggtcatcga acaaggcaag gtaatcacca ccaacttcgg tgagaataag attccgtcga
     1621 tccgcgacat cccaccgctg aaaacggtca tccaggaatt tcccgtcggc aacggcccct
     1681 atggcggcat gagcatcggc gaaccgccgg tgctggtggc ggcggcggcg atcgccaatg
     1741 ccgttcacga tgcggtgggg gtgcgggttt acgatctgcc gatcacggcg gagaaggtat
     1801 tgacggcgct caaagaatct tcggatgatt taaccacaaa gggcacaagg atcacataaa
     1861 aagatgattc ggattcggag tgttaaccgt aagaacgcaa agcgcacaat aaggattcgg
     1921 aatttggcgg ggcaatccgc cgtggttgcc ctgccggttc aattccgatt gcgaccggcg
     1981 atgttccctg ctaattcaat ctaccaagta tgaaagacga actcgccaaa agactggttc
     2041 aaagcctcaa agacgccggg atcaatttcg taacctattt gccggaaact cgcttgagcc
     2101 agatcgtgcc gctgattcgc gacgacccaa gcatggaatt gatcgcagtg gcgaacgagt
     2161 cagaggccat caccatcgcc gccggcgcca ccctcggcgg caaacaggcc gcggtatata
     2221 tggaaaatac cggcatctat acgtcctgtt acgccttgct gacggtgggg aagcaacttg
     2281 gcgtgccgtt actgttggtg atcgcctacc tgggcggtgt gccggactgg cgcaatagtt
     2341 ttctttacgc gaccatcggc aagcattcga tcccggtgct cgacggtttg ggtgtcgacc
     2401 ataccttgat cgaagatggc gtcaagctgg agatcaaaat cacagacgcg gtgcgcgcgg
     2461 caaacgcttt gcgcgctccc tacgcgctgc tgttcgccgg ggagttctcc gaatgattcg
     2521 ctatgaatgt ttgcaactgt tggccggtaa gatgaccgat caactggtcg tgacctcgca
     2581 aagcggccag cgcatcgagt ggtcgcatct atcgcgccat gagggcaacc tgctggtcgg
     2641 catgatgggc tgcgccatcg gcgtggccac cggattggcg ttggcgctgc cgcagcgaaa
     2701 agtaatcgcc ctcgactccg acggcagcgt gctgctttcc ctgttcaact tggcgacctt
     2761 gggcaacctg cagccgaaaa acctggtcgt ctatgtgttc gacaacggc
//
LOCUS       DASQKS010000151         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4282383, whole genome shotgun sequence.
ACCESSION   DASQKS010000151 DASQKS010000000
VERSION     DASQKS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4282383"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2561
                     /gene="ileS"
                     /locus_tag="VE170_03845"
     CDS             <1..2561
                     /gene="ileS"
                     /locus_tag="VE170_03845"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYJ14596.1"
                     /translation="VPGWDCHGLPIELQVEKNIGRTKKLAMSKAEIRKLCKEYAEKYI
                     AIQRDEFKRLGVLGDWDKPYRTLDPQYEAQEVRELGKFVASGALYRKKKPVYWCASCV
                     TALAEAEVEYEDHTSTSVYVKFAVIDGGTAMTADGNTYFVIWTTTPWTLPANQAIAVH
                     PKFTYRLVKTAQGALVINQELIPSVMQAIGLEAKDYDVVPGGWSGAELEGIVCRHPWL
                     DRESKIVLGDFVTQDQGTGSVHIAPGHGQEDYEVGMRYGLPVMAPVDPTGKFTAEAGD
                     LQGESVFKADGRIVQKLIDKNALLKEDKLSHSYPHCWRCKKPVIFRATEQWFISMETN
                     DLRNQALQAIDEVKWIPPWGRDRIRGMLEARPDWCISRQRSWGVPIPAVYCRKCNQAV
                     LTQAICNHVAAIFETDGSDAWFTRSVNELMPADLKCPNCGGEDFSREEDILDVWFDSG
                     VSYASVVEQDPRLGGRADLYLEGSDQHRGWFHTALLTSLATRDRAPYASVLTHGFTLD
                     GKGRKMSKSMGNTIAPQDIAKKFGAEILRLWVSAEDYREDVRISDEILNRLVEAYRRV
                     RNTARFLISNLYDFDPSKDCMPMVELDELDHWILQRTENLLSRCRDAYEKCEFHVVYH
                     SVNNFCAVELSSQYLDIIKDRLYCEGKSSTKRRAAQTALYRILDVLVHLTAPILSFTA
                     EEIWNFLPDDPERPKSVFLSQLPEANAEFADAELGERWERIFRERAEVLKALEQARSA
                     GIIGHPLDAKVVFVKRGSQPSAKLSEWVNTDRARLQDLLIVSQADNGAETAPVAKDTA
                     SYDAPLLNCVIQVSKAEGAKCERCWKYDVNVGADQNHPTVCPRCAAVLNAGAAA"
     gene            2558..3070
                     /gene="lspA"
                     /locus_tag="VE170_03850"
     CDS             2558..3070
                     /gene="lspA"
                     /locus_tag="VE170_03850"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYJ14597.1"
                     /translation="MSGKWQFVCGSLAAIIFLDQLTKIVVDRTMALYQSIPIVDGLFN
                     LTYVRNPGAAFGIFAGSAAYLRRPFLILVSILASGFIVTLLRRLDSRERGLITALTFI
                     LSGAIGNLIDRVIHGEVIDFLDVYWRNHHWPAFNVADSFITTGVALALVCLYRHKGGD
                     PFAGPAQADR"
     gene            complement(3082..>3295)
                     /locus_tag="VE170_03855"
     CDS             complement(3082..>3295)
                     /locus_tag="VE170_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYJ14598.1"
                     /translation="PKDIASVVDEYLAQCKPAGFHEVRLIHGKGKGVQRNIIRALLAK
                     HEGVESFHDAALEAGSWGATVVILKS"
BASE COUNT          714 a          961 c          930 g          690 t
ORIGIN      
        1 atgtgccggg ctgggactgc catggcctgc ccatcgaatt gcaggtcgag aagaacatcg
       61 gcaggacaaa aaaattggcc atgagcaagg ccgagattcg taaactttgc aaggaatacg
      121 ccgaaaagta tatcgcgatt caacgtgacg agttcaagcg gctcggtgtc ttgggcgatt
      181 gggataagcc ttatcgcaca ttagacccgc agtatgaagc tcaggaagtg cgcgagttgg
      241 ggaaattcgt cgcctccggc gccctctatc gaaaaaagaa accggtctat tggtgcgctt
      301 cctgcgtcac ggcgttggcg gaagccgagg tcgagtacga agaccatacg tcgacgtcgg
      361 tctacgtcaa attcgcggta atcgatggtg gcaccgccat gacggcggac ggcaacacgt
      421 atttcgtcat ttggaccacc accccgtgga ctctgccggc caaccaagca atcgccgtcc
      481 atcccaagtt tacctatcga ctggtgaaga ccgcgcaggg cgctttagtc atcaaccaag
      541 agctcatccc cagcgtgatg caagccatcg gcctggaagc caaggattat gacgttgttc
      601 cgggcggctg gagcggcgca gaactcgaag gcatcgtctg ccgccacccc tggctcgacc
      661 gcgagtcgaa aatcgtgctt ggcgacttcg tcacccagga tcaaggcacc ggctccgttc
      721 acatcgcccc cggccatggt caggaggatt atgaagtcgg catgcgttac ggcttgccgg
      781 tcatggcgcc ggtggacccg acggggaaat ttaccgcgga agccggggat ttgcagggcg
      841 agtcggtgtt taaggccgat gggcgcatcg tccaaaaatt aatcgacaag aatgcattac
      901 tcaaagaaga caagcttagc catagctacc cgcactgttg gcgctgcaaa aagccggtga
      961 tctttcgcgc caccgagcag tggtttatct ccatggagac aaacgatctg cgcaaccaag
     1021 cgctccaagc catcgacgag gtcaagtgga ttccgccgtg gggtcgcgac cggattcgcg
     1081 gcatgctcga agcgcgaccg gactggtgta tctcgcgcca gcgctcttgg ggcgtgccga
     1141 ttccggcggt ctactgcagg aaatgtaacc aagccgttct gacccaggcg atctgcaatc
     1201 atgtcgcggc gatctttgaa acagatggat cggatgcctg gttcacccgg tcggtgaacg
     1261 agctcatgcc cgccgatctc aagtgtccca attgcggcgg cgaggatttt tcccgcgaag
     1321 aagatattct cgatgtctgg ttcgattcag gggtcagcta tgcttcggtg gtcgaacaag
     1381 acccgcgtct aggcggcagg gcggatcttt atctcgaggg cagcgatcaa catcgcggct
     1441 ggtttcatac cgcgctgctg acttctctgg cgacccgcga ccgcgcgcca tacgccagcg
     1501 tgctcaccca tggtttcacc ttggacggca agggaagaaa aatgtccaag tcgatgggca
     1561 acaccatcgc gccgcaagat attgcaaaaa aattcggcgc cgagattctg cgcctgtggg
     1621 tctccgccga ggactatcgc gaagacgtcc gcatctccga tgaaattttg aatcggctgg
     1681 tcgaggccta ccggcgcgtg cgcaacaccg cgcgctttct gatcagcaac ctttatgatt
     1741 tcgatccgtc gaaggattgc atgccgatgg tcgagctcga cgagctcgac cactggatcc
     1801 tccagcgcac ggaaaatctg ctcagccgct gtcgcgacgc ttacgagaaa tgcgaattcc
     1861 acgtggttta tcacagcgtc aataacttct gcgccgtcga gctgagctca cagtatctgg
     1921 atatcatcaa agaccggctt tattgcgagg gaaagagttc gaccaaacgc cgcgcggcgc
     1981 agacggcttt atatagaatt cttgacgtgc tggtgcattt gaccgcgccg atcctatcgt
     2041 ttaccgccga agaaatctgg aacttcctgc cggatgaccc cgaacggccg aagagcgttt
     2101 ttctatcgca attgccggaa gcgaacgccg aattcgccga tgccgagctc ggtgaaagat
     2161 gggagcgcat cttccgcgag cgcgccgaag tgctcaaggc attggaacag gcgcgcagcg
     2221 ccggcatcat tggtcacccc ctcgatgcca aagtggtctt cgtcaaacgc ggcagccagc
     2281 catccgcaaa attgagcgaa tgggtgaaca ccgaccgcgc ccggctgcag gatctgttga
     2341 tcgtatcgca agccgataac ggcgctgaaa ccgctcccgt cgccaaggat actgcaagct
     2401 acgacgcgcc actgctcaac tgcgtgatcc aggtcagcaa agccgaaggc gccaagtgcg
     2461 aacgctgctg gaagtacgat gtcaatgtcg gcgccgacca gaaccatccg accgtttgcc
     2521 cgcgttgcgc ggcggtattg aacgccggag cggccgcttg agcggcaagt ggcaattcgt
     2581 ctgcggctcg ctcgctgcga ttatttttct cgatcagtta accaagatcg tcgtcgacag
     2641 gacgatggcg ctttaccagt cgattcccat cgtcgacggc ctgtttaacc taacctatgt
     2701 gcgcaacccc ggcgcggcat ttggcatctt cgccggtagc gcggcatacc tccgccggcc
     2761 gtttctgatc ttggtatcga ttcttgccag tggctttatc gtcaccctgc tgcgccgcct
     2821 tgacagtaga gaacgcggct taatcacagc gctaaccttt attcttagcg gcgctattgg
     2881 caatctcatc gatcgtgtta tccacggcga agtcatcgat ttcctcgacg tttactggcg
     2941 taaccaccac tggccggctt tcaacgtcgc cgacagtttc atcaccactg gcgtcgccct
     3001 tgcgcttgtc tgcctgtacc ggcacaaggg cggcgatccc tttgccgggc cggcacaggc
     3061 ggacagataa attttcggca actagctttt taaaattacc accgtcgcgc cccaactgcc
     3121 ggcctcgagc gcggcgtcgt gaaacgattc gacaccctcg tgctttgcca acaatgcgcg
     3181 aataatattg cgctgcacgc ctttgccttt gccgtgaatc aaacgtactt catgaaagcc
     3241 ggctggttta cactgggcga gatattcgtc aaccacactg gcgatgtctt tgggt
//
LOCUS       DASQKS010000152         8186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5543218, whole genome shotgun sequence.
ACCESSION   DASQKS010000152 DASQKS010000000
VERSION     DASQKS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5543218"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VE170_03860"
     CDS             <1..780
                     /locus_tag="VE170_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14599.1"
                     /translation="AKPDGLSLGAIYPALYMDQLVKRPEVKYDWNKFAWIGSPVSSNH
                     MMYMRADTPYKTIDDVRAAAQPPKCGTSGITSTGYYIPKLLEEAIGAKFDIVAGYQAG
                     LDVELAVERGEVVCRSFTITTFHSREPFFTWRKKNFVRVLYQTGTKRDARLKEVPTMY
                     EIIDRYKTAEPVRKLAKVILIASDLGRPIIAPPGLPADRLKILRDAFTKTVNDPAFLA
                     EAERRKLEIDPSSWEEIESLVKEAMATPPDVIERMKKVLGM"
     gene            907..1905
                     /locus_tag="VE170_03865"
     CDS             907..1905
                     /locus_tag="VE170_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14600.1"
                     /translation="MNLERVAAQTPPFYQGKTIRIIVGNLPGDTHDIFARAYSRHMGK
                     HIPGFPNILVQNMPGAGGMIAANHVYNVAKPDGLTLGSPSPALYYAQLIGSSQAKFDW
                     PKFNFIGAPERNGHLLFMRADSPYKTLDDIRKAKEPPKCAATGVGTSGHDVPKLFDEM
                     IGLKFNLITGYPGGAEMDLAIERGEAICRAITIAAFFAREPFVSWYKTGVVRVMLQTS
                     RQRNPRIPDVPTLFELMEQYKTPEIKRRQVLTYLGVGGFGAWPILATPGIPAERVAIL
                     REGFNKTMKDADFLGEAKKAGWEIRPTNGETLQALAKEVIEQPPEVIDWLKKLLNK"
     gene            1925..3022
                     /locus_tag="VE170_03870"
     CDS             1925..3022
                     /locus_tag="VE170_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14601.1"
                     /translation="MAPRLNEAFKSSKVQGFDRTLFGFLILNLLLLNLLNLDRASAQT
                     PYYLGKTIRVFVGYQPGDTHDLWARMYTRYMGKYLPGNPTFVVQNMPGAGGMIAANHV
                     FNLTKPDGLTLATVGGALYFNQLIGRAEVRFDWNRFTWIGTPEPAGLLVYMRTDAPYK
                     TLDDLRQTNDPPKCSATGLGSAGYDLPRLLEDVLGLKFKVISGYPGGADQDLAMERGE
                     VHCRAFTIEAFFGREPYFTWVKKNFVRVLLQTERQRHPKLPDTPTIYELMDQYKITAA
                     KQRMASVYLSSGRFGSRPIVSSPGLSAERAKMLRDAYAKTLKDPDFLDEIGKRSWSVD
                     PVYGEELQALAKEVTSQPPEVIEWLKKLLSK"
     gene            3139..4137
                     /locus_tag="VE170_03875"
     CDS             3139..4137
                     /locus_tag="VE170_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14602.1"
                     /translation="MNFEPASAQQTPYYQGKTIRIIVGYQSGDNHDQYARTYARFMGK
                     YLPGNPSFVIQNMPGAGSMIAANHVYNMTKPDGLTLGSFGGALIMAQLTGRKEAQFDW
                     PKYGWIGTPYRAGHLLFMRTDAPAKTIDQLRQGNDPPRCSATGVGSTGYDIPRLLEDA
                     LGMKFKIISGYPGGADQDLAMERNEVNCRAITIDGFFAREPFISWVKKGFVHVLLHTE
                     KQRHAKIPNVPTVFELMDQYKVTEAKRRLVNTYLGLWGFGSMPLVTTPGVPADRIKLL
                     REAYLKTFADPEFQEDMAKRGWEPRPVNGDELAALAKEVVNQPADVSDAIKRILSQ"
     gene            4506..6005
                     /locus_tag="VE170_03880"
     CDS             4506..6005
                     /locus_tag="VE170_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ14603.1"
                     /translation="MLEAFISGFWQTVAWPAFGYMLIGIAIGFFVGLLPGIGGLSTLA
                     LMLPFSYSIKEPISAFAFLLGMLAVTGTTGDLTSILFGIPGEGSSAALILDGYPMSKN
                     GEAGRALGAAIMSSVLGSVIGALSLALSIPIMRPLVLLFGSPELFIIAIMGITFIGTL
                     AGPSLIKGLLAGGIGLMLAAIGVDPQTGSLRYTFGTLYLWDRLDLIPVVVGLFAIPEI
                     VDLAVRGTSIASGKTEKLHGVMEGVKDAFRHFWLVVRCSLIGVYFGVLPVLGANVAQW
                     ISYAHAVQGLKDKSRAGKGAIEGLLGPAAANNSTRGGDLIPTIAFGIPGSGAMALLLG
                     AMLIVGLNPGPEMLKSRLDVTFSMVWVLILANLIVAALCFLILNHLAALTFIRGSVLI
                     PFLMFLVFIGSFTANNDLADIVVTLLFGCIGYCMVKYGWPRPPLILGLTLGKIAENYL
                     WISTAAYGAKWLLFPTVLGLIALTIFTIAYPTIKERLGKAQYERPEDAV"
     gene            6244..7908
                     /locus_tag="VE170_03885"
     CDS             6244..7908
                     /locus_tag="VE170_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14604.1"
                     /translation="MTHDVVIKNGNVIDGSGSPAFQADVAIDGDKITAIGKDLGGGKK
                     EIDAKGHVVAPGFVDSHTHMDAFVVQYPNGNPVVNYGVTSIVIGDCGASCSPVPPKPE
                     PRKVLVEYLRRVLDKYVDDKDWRWNTFGEYLSYLEGKIAINCLPLMPHSPVRLTVMGE
                     AAYQRAANPDELNAMKAMVREGMELGAIGFSTSPRGGPAIHAGTPSTFAEHDEIIELA
                     NVAAEYKGCFQFNGFQMVLQPESGVPEMLTKIKTLQIGNEFRVRPGAKAAGMESIKYM
                     EEAQKRGQDIYGVVIPYQHIRRFNLDDCFLFNGLPTWESIKNSSDLKAKLSDKETRRK
                     IEQERIACQGKLEFPEWHGWDRVVFEHLEKPELKKLEQKNMAEVARLTEKQPVDAFFD
                     LWIEDNFNSRILYHGLANAHPELLAEMIKSDTSLIGTDVGAHLDRFFWHGAPTKILGY
                     WRREKNLMSLEAAVHKITGYPAKKLRLNRGLVREGLPADITIFDADKIDDLVSAKLPD
                     QVDAQEVQRHPPGMKAVLVNGSTVVEDGQCLDVFPGKVRRQQLCIN"
BASE COUNT         1819 a         2457 c         2197 g         1713 t
ORIGIN      
        1 gccaagcccg acggcttgag ccttggagcg atctatcccg cgctttacat ggaccaactg
       61 gttaaacgac cggaggtcaa gtatgactgg aataagttcg cctggatcgg cagtccggtg
      121 agttccaacc acatgatgta catgcgcgcc gacacgccct acaaaaccat cgacgacgtg
      181 cgtgccgctg cgcaaccgcc gaaatgcggc accagcggca ttacctcgac cggctactac
      241 attcccaaac tcctggaaga agccatcggc gccaaattcg atatcgtcgc cggctatcaa
      301 gccggcctgg atgtcgagct cgccgtcgag cgcggcgaag tggtctgccg gtcattcacg
      361 attacaacct ttcactcccg cgagccgttt tttacctggc gcaaaaaaaa cttcgtgcgc
      421 gtgctctatc aaaccggcac caaacgcgac gcgcgtctca aagaagtgcc gaccatgtat
      481 gagatcatag accgctacaa gacggcggaa ccggtgcgca agcttgctaa agtgatctta
      541 atcgccagcg atctcggccg gccgatcatc gctccgcctg gactccccgc ggatcgtctc
      601 aaaattctcc gcgatgcttt caccaagacg gtcaacgacc cggctttctt agccgaggcg
      661 gaacgacgca agctcgaaat cgatccatcg tcttgggagg agatcgagtc gctggttaaa
      721 gaagctatgg ccacgccgcc cgatgtgatc gaacgcatga aaaaagtctt gggcatgtaa
      781 tggcaaccac gtgtgaggtt ttgcatgacg cgtctaacaa aatactggtt caaaagttca
      841 agggttcaag ggttcaatcg gactcttttc gggtttctta ttctgaccct tgtatttttg
      901 aacccgttga accttgaacg agtggcggcg cagacgccgc ctttctatca aggcaagact
      961 attcgtatca tcgtcggcaa tctgcccggc gatacccatg acatatttgc ccgcgcttac
     1021 tcgcgtcaca tgggcaaaca tattcccggc ttcccgaata tcctggtgca aaacatgccg
     1081 ggcgccggcg gtatgatcgc cgccaatcat gtctacaatg tcgccaagcc cgatgggctg
     1141 acgctcggtt caccgtcgcc ggcgctgtat tacgcccagc tgatcggcag cagccaagcc
     1201 aaattcgatt ggcctaaatt caatttcatc ggcgcgccgg aacgcaacgg ccatttgttg
     1261 ttcatgcgcg cggactcgcc ttacaagact ctcgacgata tccgcaaagc caaggagccg
     1321 ccaaaatgcg ccgccaccgg agtcggcacc agcggccacg acgtgcccaa gctgtttgac
     1381 gagatgatcg gtctgaagtt caacctgatc accggttatc ccggcggcgc ggaaatggat
     1441 ctagccatcg aacgcggcga agcgatttgc cgcgccatta ccatcgcagc cttcttcgcc
     1501 cgcgagccct ttgtcagctg gtacaaaacc ggcgtggtgc gcgtcatgtt gcaaacgtcg
     1561 cggcagcgca acccaaggat ccccgacgtg ccgacgttgt tcgagctgat ggagcagtac
     1621 aaaactccgg agatcaagcg ccgccaggtg ctaacctatc tcggcgtcgg cggtttcggc
     1681 gcctggccca ttctcgcgac gccgggaatt ccggccgagc gcgtggcgat cctccgcgag
     1741 ggattcaaca agactatgaa agatgccgac ttcctaggcg aggcgaaaaa agccggctgg
     1801 gaaatccgtc cgactaacgg cgaaaccttg caagcactcg ccaaagaagt catcgaacaa
     1861 ccgccggaag tgatcgattg gctgaaaaaa ctacttaaca aataatctaa caatgagaat
     1921 ctccatggca cctcgactaa acgaagcgtt caagagttca aaagttcaag ggttcgatcg
     1981 aactcttttc ggctttctta ttctgaacct tctactcttg aacctcttaa accttgatcg
     2041 ggcgtctgcc cagacgcctt attatttggg taaaaccatc cgcgtcttcg tcggttatca
     2101 gccgggggat acccatgatc tatgggcgcg catgtacacg cgctatatgg gcaagtactt
     2161 gcccggcaat ccgacctttg tcgtgcaaaa catgcccggt gccggcggca tgatcgccgc
     2221 caatcatgtt ttcaatctga ccaagcccga cggcctgacg cttgccaccg tcggcggcgc
     2281 gctttacttc aatcagttga tcggccgtgc ggaagttaga tttgactgga acaggttcac
     2341 ttggatcggc acgccggagc ccgccggcct gctggtctac atgcgcaccg acgcgccgta
     2401 caagaccttg gacgacctgc gccagaccaa tgatccgccg aaatgttccg ccaccggctt
     2461 gggcagcgcc ggctacgact tgccgcggtt gctcgaagat gtcttagggc ttaaattcaa
     2521 agtcatcagc ggctatcccg gcggcgccga ccaggatctc gccatggaac ggggcgaagt
     2581 ccactgccgg gcatttacca ttgaagcttt tttcggccgc gaaccctatt tcacctgggt
     2641 gaagaaaaac ttcgtgcgcg tgctgttgca aaccgaacgg cagcgccacc cgaagctgcc
     2701 cgacacaccg accatctacg aactgatgga ccagtacaag attaccgcgg ccaagcagcg
     2761 catggcgagc gtctatctca gcagcggccg gttcggctcc cggccgatcg tttcgtcgcc
     2821 ggggttatcg gccgaacgcg ccaaaatgct gcgtgacgcc tacgcgaaga cccttaaaga
     2881 tcccgacttt ctcgacgaga tcggcaaacg gagctggagc gtcgacccgg tatacggcga
     2941 agaattgcag gcgctcgcca aagaagtcac cagccagccg ccggaagtga tcgagtggtt
     3001 aaaaaaattg ttgtcgaaat aattcgcttc gaagcgaggt cggccatgaa aagattttcg
     3061 ttcaagagtt caagggttca atcgtttaat cggatcgttt tcggatttct tgttctgacc
     3121 cttgaacttt tgaacccttt gaactttgaa ccggcgtctg cgcagcagac gccctactat
     3181 caaggcaaaa ccatccgcat catcgtcggc taccagtcgg gcgacaatca tgatcaatat
     3241 gcgcgcacct acgcgcgttt catgggcaag tatcttcccg gcaatcccag ctttgtgatt
     3301 cagaacatgc cgggggccgg ttcgatgatc gccgcaaatc atgtctacaa catgaccaag
     3361 cccgacggac tgacgctggg ttccttcggc ggcgcgctga ttatggcgca gctgaccgga
     3421 cgcaaggaag cgcagttcga ctggcccaag tatggttgga tcggcacgcc ctaccgtgcc
     3481 ggccacctgc tcttcatgcg taccgacgcg cctgccaaaa cgatcgacca attgcgccag
     3541 ggaaacgatc cgccgcgctg ttcggccacc ggcgtcggca gcaccggcta tgacattccc
     3601 cgactgctcg aggatgccct cggcatgaaa tttaaaatca tcagcggtta tcccggcggc
     3661 gccgaccagg acctggcaat ggaacgaaac gaagtcaact gccgcgccat caccatcgac
     3721 ggcttcttcg cccgtgaacc gttcatctca tgggtcaaga aaggcttcgt gcacgtgctg
     3781 ctgcataccg aaaaacagcg ccacgccaag attcccaacg tgccaaccgt cttcgaactg
     3841 atggatcagt acaaagtcac cgaggccaaa cgccgcctgg tcaataccta cttgggactt
     3901 tggggcttcg gctccatgcc gctggttacc accccaggag tgcccgcgga tcggatcaag
     3961 ctgctgcgcg aagcgtattt gaaaacattt gccgatccgg aattccagga agacatggcg
     4021 aagcggggct gggaacctcg cccggtgaat ggtgacgaac ttgccgctct cgccaaagaa
     4081 gtggtcaacc agccggcgga cgtgagcgac gcgatcaaac gaattttaag ccagtaaata
     4141 agcagatttt acaaaaaggt aataccgggc caccttccca gatgcattcc ctgagctttt
     4201 cgacaaaccc cagggtcttt ggacatcttg gcgaacgctt cgcgcaataa cttcacgccc
     4261 tttggcggca tctcggtgtc gacagtatcg tgggcgaaga accgcaaaaa tttcgctaag
     4321 gaagtagcaa accagcagcc gaaggtcgct gctgcgttgg aacgaatcct gggtccatca
     4381 tgccggtaat gccttccatg aatgacctcg ggcaataaaa gttttgcggc aaatttggtt
     4441 gacggtaatc gtaagataag gtaatgagca tcagccgcta atcttaacga ccccgggaga
     4501 atttcatgct tgaggctttt atcagtgggt tttggcaaac tgtcgcatgg ccggccttcg
     4561 gttacatgct gatcggcatc gccatcggtt tctttgtcgg tctgttaccg ggcatcggcg
     4621 ggctatcgac gctggccttg atgctgcctt tttcctattc gatcaaagaa ccgatctccg
     4681 cttttgcctt tctcctcgga atgttggcgg tcaccggcac caccggcgat ctaacttcga
     4741 ttctttttgg gatccccggg gagggatcct cggccgcgct gattctcgac ggctacccga
     4801 tgtcgaaaaa cggcgaagcc ggccgcgccc ttggtgcggc gatcatgagc tccgtgcttg
     4861 gctccgtgat tggcgctctg tcgctggccc tgtcgatccc gatcatgcgc ccgctggtcc
     4921 tacttttcgg ctcgccggaa ttatttatca tcgcgatcat gggcatcacc ttcatcggca
     4981 ccttggctgg cccatcgctg attaaagggt tgctggccgg cggcatcggc ctcatgctcg
     5041 ccgccatcgg cgtcgatccg cagaccggct cgctgcgtta cacattcggc acgctgtatt
     5101 tgtgggaccg gctcgatctc atcccggtgg tcgtcggcct gttcgccatc cccgagatcg
     5161 tcgatctcgc tgtccgcggc accagtatcg ccagcggcaa aacggaaaag ctgcacggag
     5221 tcatggaagg cgttaaagat gcctttcgcc acttctggct ggtcgtccgt tgcagtttga
     5281 ttggcgtcta cttcggtgtc ctgccggtcc ttggcgccaa cgttgcccag tggatctctt
     5341 acgcccacgc cgtgcagggg ttgaaggata aatcccgcgc tggcaagggc gccatagaag
     5401 gtcttctcgg tcccgccgca gcgaacaatt cgacccgcgg cggcgatctg atcccgacca
     5461 ttgcattcgg catccctgga agcggcgcca tggctttgct gctcggagcc atgttgatcg
     5521 tcggcttgaa tcccggtccg gagatgctca agtcacgcct cgacgtgacc ttctcgatgg
     5581 tctgggtact gattctcgct aacctcatcg tcgctgccct gtgtttcttg attctcaacc
     5641 acctcgccgc gctgaccttc atccgcggtt cggtgctgat cccctttctc atgtttttag
     5701 tctttatcgg ttcatttacc gcaaataacg acctcgccga catcgtcgtc accttgctct
     5761 tcggctgtat cggctactgc atggtcaaat acggctggcc gcgcccgccg ctgatattag
     5821 gcctgaccct cggcaaaatc gccgaaaact atttgtggat ctccaccgcc gcctacggcg
     5881 ccaagtggct gctgttcccg accgtgctcg gactgatcgc gctgacgatt ttcaccatcg
     5941 cctacccgac catcaaagaa cggttgggca aagcgcaata cgaacggcct gaagacgcag
     6001 tttaaattta aactccgcac accattcgaa ttcgtcaata gtttagcgtc aagccgtcca
     6061 ggattcatcg gaacccgggc gcggcgctga gtcatgcgcg atggcggcag gaaactgtaa
     6121 cgctccattg cgctgctgaa gacgctggac aatagtcgcc aaattgaatt aggcagttga
     6181 cagccagtcg ctttcagtgg gataagcaat gcatctcaca gatccaaggg aggacaccca
     6241 attatgactc atgatgtagt cattaaaaac ggcaacgtta tcgacggcag cggcagcccg
     6301 gctttccaag cggatgtcgc catcgacggc gataagatca ccgccatcgg caaagatctc
     6361 ggcggcggca aaaaagaaat cgacgccaaa gggcatgtgg tcgcgcccgg gttcgtcgat
     6421 tcgcacactc acatggacgc ctttgtcgtc cagtatccca acggcaaccc ggtggtgaat
     6481 tatggcgtca ccagcatcgt catcggtgat tgcggcgcgt cctgctcgcc ggtcccgccc
     6541 aagcccgaac cgcgcaaagt cttggtcgaa tatctgcgcc gggtgctcga caagtatgtc
     6601 gacgacaagg attggcgctg gaataccttc ggcgaatacc tgagctatct cgaaggcaag
     6661 atcgccatca actgtttgcc gctcatgccc cactcgccgg tgcgtttgac cgtcatgggc
     6721 gaggccgcct accagcgtgc cgccaatccg gatgaactga acgccatgaa ggcgatggtg
     6781 cgcgaaggca tggaactggg cgccatcggt ttctcgacca gcccgcgtgg cggcccggcg
     6841 attcacgccg gcacgccgag cacttttgcc gaacatgacg agatcatcga actcgccaac
     6901 gttgccgccg aatacaaagg ctgctttcag ttcaatggct tccagatggt cctccagccc
     6961 gaatccggcg tgcccgaaat gttgaccaag atcaaaacgc tgcaaatcgg caatgaattc
     7021 cgtgtccgtc ccggcgctaa agccgccggc atggaatcga tcaaatacat ggaagaggcg
     7081 cagaaacgcg gccaggatat ttacggcgtg gtcattccct accagcatat tcgccgcttc
     7141 aatctggacg actgctttct attcaacggc ctgccaacct gggagtcgat caaaaattcc
     7201 agcgatctca aagccaagct cagcgacaaa gagacgcgcc gcaagatcga gcaggagcgc
     7261 atcgcctgcc agggcaaact cgaattcccc gaatggcatg gctgggaccg ggtcgtgttc
     7321 gagcatttgg aaaaaccgga gttaaagaaa ctcgaacaaa agaacatggc cgaagtcgcc
     7381 cgcctcaccg aaaaacagcc ggtggacgct ttcttcgacc tctggatcga ggacaacttc
     7441 aactcacgga tcctttatca cggcctcgcc aacgcccatc cggaactttt agccgagatg
     7501 atcaagtccg acaccagcct gatcggcacc gacgtgggcg cgcatctcga tcgcttcttc
     7561 tggcacggcg caccgaccaa gatcctcggc tactggcgcc gcgagaaaaa tctcatgagc
     7621 ttggaggccg cagtgcataa gatcaccggc tatccggcga aaaagctgcg cctcaaccgc
     7681 ggtctggtgc gcgaagggtt gccggcggac atcaccatct tcgatgccga taagatcgac
     7741 gatctggtca gcgcgaaact cccagatcag gtcgacgccc aggaagtgca gcgccatccg
     7801 cctggaatga aagccgtgct ggtcaacggc agcaccgtag tcgaggacgg ccagtgcctc
     7861 gatgtattcc ccggcaaagt gcgccgtcag caactctgta ttaattagca tcaccgcact
     7921 cgcggccggt ttgcgacctt ttccttcacc tcgccctctc ccctctcggg agagggcagg
     7981 ggcgagggaa ttttaattta attcttagaa ggaacatgac aacaatgaaa gcctgtgcca
     8041 tcgacattca tcaccactat gtcccggaga gtttactgga ggaggcgaaa aaacgcggcc
     8101 agcatctcgg cgtgacattg accgagaagg acggtaatcg ctcgctctcc ttcgccggtg
     8161 gaccgccatt cctactgcat ccggag
//
LOCUS       DASQKS010000153         8351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3862893, whole genome shotgun sequence.
ACCESSION   DASQKS010000153 DASQKS010000000
VERSION     DASQKS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3862893"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VE170_03890"
     CDS             <1..1097
                     /locus_tag="VE170_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14605.1"
                     /translation="GIVYSVAYLSGAVLTAVWGYLSDRYGRRNILMLLAVLTIISNGI
                     FVLFNGLGFILLAVIIANVGAGGSGGGGSGGGPFNPVEEALLAEKCHPENRNQIFALN
                     AFVGSVMGAIGALAAGLPQFLQERAGWDSVSSYKPLFVLTILFSVALLFVYRAIDERH
                     QPRQVEKTISHTTGVFVTKMSILAFVDNFGAGMAGGLVAYWFFLRFGVDLKSLGLLFF
                     ISNFLAALSFLSAPIIARYLGIVRTMAFSHGFASLIYLSIPFAPTFFLAATLLAARSF
                     FAYMDNPLRASFTMAMVRSSERGSAAGVTSLARAVPFGISPTIATYLMQNISLTLPLF
                     IGGGLQLVNDVAFYLMFRHVRPPEEMNPKP"
     gene            1228..1635
                     /locus_tag="VE170_03895"
     CDS             1228..1635
                     /locus_tag="VE170_03895"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HYJ14606.1"
                     /translation="MATRRPSRPTGAVGFIERLAEQRILEAQRKGEFDDLAGKGKPLD
                     LADHSDVPADLRMAYHVLKNANVLPPEAELMKDIRILEDLLKHVEDEGERKSLAKSLQ
                     WKMIRLDILKRRSMNLASVRDYSRKIVAKLSRR"
     gene            1736..2242
                     /locus_tag="VE170_03900"
     CDS             1736..2242
                     /locus_tag="VE170_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14607.1"
                     /translation="MSEKSPINIKKIGHIVYACSDIERTSKFWTEIMGFKFSDKNEGG
                     MVFFRNANDHHTIALVQATEKNQLPKRGEVGFDHMALEVATVSELFKIRDFLKAKGVT
                     IFYEGRRGPGGNPGLEFYDPDGYKIEIYAAMDQIGTDGKSRPANEWSRATTLEEAVNN
                     PLPGAEYN"
     gene            2330..3403
                     /locus_tag="VE170_03905"
     CDS             2330..3403
                     /locus_tag="VE170_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14608.1"
                     /translation="MISIRRLHKQFLVSDGVVAAIKSLDLAIAEGELFVIVGASGSGK
                     TTLLRCIAGLEVPDGGEIRIDGKIVSSDTPPTWIPPQQRRLGMVFQSYAVWPHLTVWQ
                     NIALPLAEGSQRIAKSEVAERVKEALGLVQLAEQADRPATLLSGGQQQRVALARAIAV
                     NSRLLLMDEPLSNLDARLREDVRGKIRDLAKQLGSTVLYVTHDQIEAMAIADKIAIMQ
                     AGELLQVGTPAQIYHKPISADVADFFGSVNWLDGEIVAAGVADSPIGKLAVEPNAKPG
                     QKVRLGFRPENLRALTGASQTTGFAGQIVSSTFLGDQFTFCADANGRQLFGKNRTLPD
                     SSNGLTRFIVDAADIMVFARRDP"
     gene            3434..4504
                     /locus_tag="VE170_03910"
     CDS             3434..4504
                     /locus_tag="VE170_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14609.1"
                     /translation="MIARLTAVVIALLWLAAQAGATTLNELIEGARKEGTLRGQWGQA
                     SFGGSEGFKEIVANMNRKYKLDLKGQYTPGPDMQALMLRIIQEAAAGQPASTDVYLGN
                     AQAMLDALNSKALKPMTFSQFIQDKPASEGKFNPIAADGTHVAVATAVVGVQYNTNAV
                     KGNDIPKRIEDVLNPKWKGKIASTPYAAGMREFATPDFLGREKMVDFTRKLSKQIAGL
                     MRCGETERITSGEFIMLAFTCGGNDVNVAKRTGAPVGHSVMEEGTVLHMRYAAVPKNS
                     RSPNAGALLIHYLMSPEGQELLWRIDGLDLHLFPGSQTKRELDNVRAAKGKIIINSPA
                     WLASFKDYAAIQKELEGILRQK"
     gene            4627..6366
                     /locus_tag="VE170_03915"
     CDS             4627..6366
                     /locus_tag="VE170_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ14610.1"
                     /translation="MTATASRLRSFFAGTQTSPALLSLAALPMAVILVLVIAMFWVSL
                     QKGALGTASAIYTLANYRDVFADPFIIGVAVNTLIFTVTATLVALLFGLPVAWLVERT
                     TLPGKTLLYAIMTLGLLIPGIYTAMGWTLIAHPRIGLVNRWLIDLFNLDAGPINIATP
                     IGMGFVQGLSLAALAFILTSQMFRAMNPSLEEAARVHGMSFSRIMRRITLPLARPGIL
                     AAVIYIFTIGIATFDIPAILGLGNRVYLLSTFIYLKVHPQGAGLPEYGITGVVGAFMI
                     IIAGLLTLWYSQVLRQGHRFEVVTGKGYKPTLIPLGRWSLAAWAFIFVYALFSKLLPL
                     LLIAYAALTPYFVPPSMEALRHLSFSHFHGMDWELVLRGLKNTALLVTVVPLAVLLLA
                     FALSWLIVRSRSRLRYVLEFGAFLPQALPEVILAIGALLLALFVIGTTIPLYGSVWII
                     AIVYTVARLAFATRALNGSLLQIHRELEEAAFVSGLSTARTAWRVLFPLIRPTLFSVW
                     IWTALLVYRELTAAVFLAVHDNITLPAVIWSYWYSGSINKASAVTLLMTLVLAPLIFV
                     AWWFGRRSQISLN"
     gene            6444..7091
                     /locus_tag="VE170_03920"
     CDS             6444..7091
                     /locus_tag="VE170_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYJ14611.1"
                     /translation="MDTEILLDIIRHRRSVRVYKTGKVSDQQLENILEAARWAPSGAN
                     TQPWEFVVTRDRKKMKKVREIYSQEWRDRKKEDPVHYKGLKKDYVGDVSVLVLACGEA
                     RTKQVYLTTRQAGDREKLFQASIANAVQQAMLAAASMNLGTVWVSVREEVEPELRELF
                     NVPEPLRLLWVMPIGHASSWPKAKPRRPIGAFTHYEVYDRKKLRNDTDIHAWPKN"
     gene            complement(7170..>8351)
                     /locus_tag="VE170_03925"
     CDS             complement(7170..>8351)
                     /locus_tag="VE170_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYJ14612.1"
                     /translation="SRGEEVLVQVAKDPLGTKGARVTSHISLPGRYMVFMPGNKHIGI
                     SRRIESDEERKRLKEIAQSLGTEEGGFILRTACEGLSKREIQHDLGFLQKLWKSIQKK
                     AESAPSPSLIHQDLDLIARAIRDFFTTDTEQVLIDSPKDHRRIVDFARHFMPRLKSKI
                     NLYSDKEPLFEQLGIEEKITKALDPRAWLRSGGYIIIERTEALTAVDVNTGRFVGKRS
                     QEETILKTNLEAVQEVVRQLRLRNAGGLIIIDFIDMEREANRKKVYDALKEALKKDKA
                     RTNILKISELGLVEMTRQRTRESLENQLLRSCPHCDGRGRIKSPVTVAYDVLRAIKRH
                     HTGMDNGRNIVVRLHPDVANFLYDEKNNSLENLERELNRRIIIKASQELHHEKYDISE
                     S"
BASE COUNT         1656 a         2544 c         2360 g         1791 t
ORIGIN      
        1 ttgggattgt ctactccgtc gcttatttga gcggcgcggt gctgaccgcg gtctggggat
       61 atctgtcgga tcgttacggg cgcagaaaca ttctcatgct gctggccgtt cttacaatca
      121 tctcgaatgg catttttgta ttgtttaacg ggttgggttt cattctcctc gcggtcatca
      181 tcgccaacgt cggcgccggt ggatcgggtg gcggcggctc gggcggcggc ccgttcaatc
      241 ccgtggagga agccctgcta gcggaaaaat gccatccgga gaatcgtaat cagattttcg
      301 cgctgaacgc ctttgtcggc tcagtgatgg gcgccatcgg cgccctggcc gccggtttgc
      361 cgcaatttct tcaggagcgc gccggctggg actccgtgtc ctcgtataaa ccgctctttg
      421 ttctgaccat tctattcagc gtcgccttgt tgttcgtcta tcgtgcgatc gacgaacggc
      481 accaaccgcg ccaagtcgaa aagacgattt cacacaccac cggcgtgttt gtcaccaaga
      541 tgtcgatcct agcgttcgtc gacaacttcg gcgccggcat ggcgggcgga ctggttgcgt
      601 actggttctt tctccgcttc ggcgtcgacc tcaaatctct cggtctgctt tttttcatct
      661 ccaattttct cgccgccctg tcatttttaa gcgcgccgat catcgcgcgc tacttgggca
      721 tcgtgagaac catggccttt tcccatggct tcgcttcgtt gatctatctt tcgattccct
      781 ttgcgccgac tttctttctg gcggcgactc ttctcgcggc acgctcgttc ttcgcctaca
      841 tggataaccc gctgcgcgct tcgttcacca tggcaatggt ccgatcgagc gagcgcggct
      901 cggccgccgg cgtcaccagc ttggcgcgag ccgtgccttt tggcatcagc ccgacgatcg
      961 cgacatattt aatgcaaaac atctctctca cgcttcccct attcatcggc ggcggtttac
     1021 aattggttaa cgacgtggcg ttttacctaa tgttccgcca cgtccggccg cctgaggaga
     1081 tgaatccgaa accgtaagga gttaaggtaa ggagtcaggt gcaaccccag gaaagaacga
     1141 cgaagctatc gggatgaatc cgacggcttg aacgatttga ccggagcgca gcgattgaac
     1201 ggctttaacc attgaccttt tttaaccatg gcaactagac gcccatcccg acccaccggc
     1261 gccgtcggtt tcatcgaacg gctcgccgag caacgcatcc tcgaggccca gcgcaaaggc
     1321 gagttcgacg atcttgccgg taaaggcaag ccgctggatc tggccgacca cagcgacgtg
     1381 ccggcagatt tacgcatggc ctaccatgtg ttgaaaaacg ccaacgtgct gccgccggaa
     1441 gccgagctga tgaaagacat tcgcatcctc gaagatctgc tcaagcatgt cgaagacgag
     1501 ggcgagcgga aatccctggc caagagtctc cagtggaaaa tgatccgcct ggatatcctc
     1561 aagcgccggt cgatgaatct cgccagcgtg cgcgattata gccgcaagat cgtcgccaag
     1621 ttgtcgcgcc gttagcaatt gaattagctt tgccttggcg aattttccgt ggtagaaatc
     1681 gcctacctta aataccggcg tccactcgcc gtttaccgat gaaaggagca cagttatgtc
     1741 agagaagtct cccatcaaca tcaagaagat cggccacatc gtctatgcgt gcagcgacat
     1801 cgagcgcacc tcgaagtttt ggaccgagat catgggcttt aagttttccg acaagaacga
     1861 gggcggcatg gtgtttttcc gcaacgccaa tgaccatcac acgatcgcct tggtgcaggc
     1921 gacggaaaaa aaccagttgc ccaagcgcgg cgaagtcggc ttcgatcaca tggcgttgga
     1981 agtcgccacc gtgtcggagc tgttcaagat ccgcgatttc ctgaaggcca agggggtgac
     2041 gattttctac gagggccggc gcggccccgg cggcaatccc ggcctcgagt tctacgatcc
     2101 cgatggctac aagatcgaaa tctacgcggc catggatcag atcggcaccg acggcaagag
     2161 ccgtcccgcc aatgaatgga gccgcgcgac caccttggaa gaagcggtca acaatccgct
     2221 gcccggcgcc gagtataact gaccctccag ccatcctatg ccggaagtcg gttgccggtt
     2281 ttcgccgctg gactggagac cggcaactga ccacggagaa cccacttaga tgatctccat
     2341 tcgccggctg cacaaacagt tcctcgtcag cgacggcgtg gttgcggcga tcaaatccct
     2401 cgatttggca atcgccgagg gtgaactgtt cgtcatcgtc ggcgccagcg gttcggggaa
     2461 aaccaccctg ctgcgctgca tcgccgggct ggaagtgcct gacggcggcg agatccgcat
     2521 cgacggtaaa atcgtttcct cggatacgcc gcccacttgg atcccaccgc agcagcgccg
     2581 gctcggcatg gtgtttcaat cctacgccgt ctggccgcat ttgacggtat ggcaaaatat
     2641 cgccctaccg ctggccgagg gcagccagcg catcgccaaa agcgaagtcg ccgagcgggt
     2701 caaagaagcg ctaggtctgg tccaattggc agaacaggcc gaccgtccgg cgactttgct
     2761 gagcggcgga cagcagcagc gcgtcgccct ggcgcgcgcc atcgcggtga actcgcgttt
     2821 gttgctcatg gacgagccgc tgagcaatct ggacgcccgc ctgcgcgaag acgtgcgcgg
     2881 caaaatccgc gatctcgcca aacagctggg ctccaccgtt ttgtatgtaa cccacgatca
     2941 aatcgaggcc atggcgatcg ctgacaagat cgccatcatg caggccggcg agctcttgca
     3001 agtgggcacg ccagcgcaaa tttatcataa gccgatcagc gccgacgtcg ccgacttctt
     3061 cggctcggtc aattggctcg atggcgagat cgtcgccgcc ggtgtcgccg actcgccgat
     3121 tggcaagctc gcagtcgagc ccaacgccaa gccggggcaa aaggtgcgcc tgggctttcg
     3181 cccggaaaat ctgcgcgccc ttaccggcgc cagtcaaacc accggcttcg ccggccaaat
     3241 cgtctcgagc acttttctcg gcgatcagtt tacattttgc gccgacgcca acggccgtca
     3301 gctgttcggc aagaaccgaa cacttcccga cagttcaaat ggattaaccc gattcatcgt
     3361 ggatgcggcg gatattatgg tttttgcccg ccgcgatccg taatcgtccg aggacaaccc
     3421 aggaggacag accatgatcg caagactcac ggcagtggta atcgccctgt tatggcttgc
     3481 cgcccaagcc ggcgccacga ctttaaatga attgatcgaa ggcgcgcgca aggaaggcac
     3541 cttgcgcggc caatggggtc aggcaagctt cggcggcagc gaaggtttca aagaaatcgt
     3601 cgccaacatg aaccggaaat ataaactcga cctgaagggc cagtacacgc cgggtcccga
     3661 tatgcaggcg ttgatgttgc ggattattca agaagccgcg gccggccagc cggcgagcac
     3721 cgatgtctat ctcggcaacg cgcaggcgat gctcgacgcc ttgaacagca aagcgctcaa
     3781 gccgatgacc ttcagtcaat tcatccagga caagccggcg agcgagggca agttcaaccc
     3841 gatcgccgcg gacggcaccc atgtggcggt cgccaccgcc gtggtcggag tgcaatacaa
     3901 caccaacgcg gtcaagggca atgacatacc caagcgcatc gaagacgtgc tcaatcccaa
     3961 gtggaaaggc aagatcgcgt cgacgcccta cgccgccggc atgcgcgagt tcgccacgcc
     4021 ggattttctc ggccgggaga aaatggtcga cttcacgcgc aagctgtcca aacagatcgc
     4081 tggcctgatg cgctgcggcg aaaccgaacg catcaccagc ggcgagttca tcatgctggc
     4141 gtttacctgc ggcggcaacg acgtcaacgt cgccaaacgc accggcgccc cggtgggcca
     4201 tagcgtcatg gaagaaggca cggtgctgca catgcgttat gccgccgtgc caaagaattc
     4261 gcgctcgccc aacgccggcg cgctgttgat tcattatctg atgagccccg aaggccaaga
     4321 attgctctgg cgcatcgacg gtttggattt gcatttgttc cccggctcgc aaaccaagcg
     4381 cgagctcgac aatgtccgcg ccgccaaagg caagatcatc atcaactccc cggcatggct
     4441 ggcgtcgttc aaagactacg cggcgatcca gaaagagctg gaaggcattc tgcgtcagaa
     4501 gtagggaagc gagctggagt gttggagtaa tggagtgatg gagtattggt tcggattccc
     4561 attccaaacc taacactcca gtactccaac actccgatac tccaccactg catcactcca
     4621 atcctcatga ccgctaccgc ctcacggctg agaagcttct tcgccggcac ccagacctcc
     4681 ccggcgctgc tgtcgctggc cgctctaccg atggcggtga tcctcgtgct cgtcatcgcc
     4741 atgttttggg tcagcctgca gaaaggcgcc ctcggcaccg ccagcgcgat ctacacactg
     4801 gccaactacc gcgacgtgtt tgccgatccg ttcatcatcg gcgtcgccgt caacactttg
     4861 atcttcacgg tcacggcgac cctggtcgcc ttgctgttcg gcttgccggt ggcctggctg
     4921 gtggagcgca ccaccctgcc gggaaagact ttactctacg ccatcatgac cttgggcttg
     4981 ctcataccgg gtatttacac ggccatgggc tggactttga tcgcccaccc gcgcatcggt
     5041 ttggtcaatc gctggctgat cgatctgttc aatctcgatg ccgggccgat caatatcgcc
     5101 acgcccatcg gcatgggctt cgttcaaggg ctgagcctcg ccgccctggc gttcatttta
     5161 acctcgcaaa tgtttcgcgc catgaatccg tcgctggaag aggcagcgcg ggtgcatggc
     5221 atgagcttta gccgaatcat gcgccgcatt accttgccgc tggcgcgacc gggtatcctg
     5281 gcggcggtga tttatatctt caccatcggc atcgccacct tcgatattcc ggcaatccta
     5341 ggtttaggca accgggttta cctgcttagc acttttattt atctcaaagt gcatccccag
     5401 ggcgccggcc tgcccgagta cggcatcacc ggtgtcgtcg gcgccttcat gatcatcatc
     5461 gccggtctcc tgacactctg gtacagccag gtgctgcgtc agggacaccg cttcgaagtc
     5521 gtcaccggca agggctacaa accgaccttg atcccactcg gccgttggtc gctggccgcc
     5581 tgggctttta tatttgttta cgcgctgttc tcgaaactat tgccgctgtt gctgatcgcc
     5641 tacgccgcgc tcacgccgta cttcgtgccg ccgtccatgg aagcgctgcg gcatctctcc
     5701 ttcagccatt ttcacggcat ggactgggag ctggtgctgc gcggtttgaa gaataccgcg
     5761 ctgttggtca ccgtggtgcc gctggcggtg ctcctgctcg ccttcgcgct ctcctggttg
     5821 atcgtgcgct cgcgcagccg gctgcgctat gtgctggagt tcggcgcctt cctgccccaa
     5881 gccctgcccg aagtgatcct tgccatcggc gctttgttac tcgcgctgtt tgtcatcggc
     5941 acgactatcc cgctctacgg ttcggtatgg atcatcgcga tcgtctacac ggtcgcccgc
     6001 ctggctttcg ccacccgcgc gctcaacggc tcgctcttgc aaatccatcg cgagctcgag
     6061 gaagcggctt ttgtctccgg cttgtcgacc gcccgtaccg cttggcgcgt attgtttcca
     6121 ttgatccggc cgacgctttt ttccgtgtgg atttggacgg cattgctggt ctaccgtgaa
     6181 ctcaccgcgg cggtctttct cgccgtccac gacaatatca ccttgcccgc ggtgatctgg
     6241 agctactggt acagcggcag cataaacaag gcttcggcgg tgactctact gatgacactg
     6301 gtcttggcgc cgcttatttt cgtcgcctgg tggttcggcc ggcgcagcca aatctcgctc
     6361 aattgacacg gacttcaggc gccgggatgg gtcaagtgaa caacctgaaa caaacacaat
     6421 cgatcgctat caaaaggaga aacatggaca cggagatttt gctcgacatc atccgccacc
     6481 gacgcagcgt gcgcgtctac aagaccggca aggttagcga ccaacagctg gagaatatcc
     6541 tggaagcggc gcgctgggcg ccatccggcg ccaacaccca gccgtgggaa ttcgtcgtca
     6601 cccgcgaccg caaaaaaatg aagaaggtgc gcgagatcta ttcccaggag tggcgcgacc
     6661 ggaaaaaaga agacccggtc cactacaagg gattgaaaaa ggattatgtc ggcgatgtca
     6721 gcgtgctcgt gctggcctgc ggcgaggcgc gcaccaagca agtctatttg accacgcgcc
     6781 aggccggcga ccgggaaaaa ttatttcagg cgagcatcgc caacgccgtg cagcaagcca
     6841 tgctcgccgc cgccagcatg aacttgggca ccgtgtgggt gtccgtgcgc gaagaagtgg
     6901 aaccggagtt aagagaacta ttcaatgtgc cggagccatt gcgcctgctc tgggtcatgc
     6961 ccatcggcca cgccagcagt tggcccaaag ccaaaccccg ccgcccgatc ggcgccttca
     7021 cccactacga agtctacgac cggaagaaat tgcgcaacga caccgacatc cacgcctggc
     7081 cgaaaaactg aaaaggcggg tggcataccc gcctacgtat cgtgatggtt ttgtgttgta
     7141 tgcgttcttt gcggccaagc tttttaatcc tacgattctg aaatatcgta tttctcgtga
     7201 tgcagctcct ggctcgcttt gatgatgatg cggcggttga gttcgcgctc gagattttcc
     7261 aggctgttgt ttttttcgtc gtaaagaaaa ttcgccacat cgggatgcag gcgcacgacg
     7321 atgttgcggc cgttatccat gccggtgtga tgccgtttga tcgcgcgcag gacgtcgtag
     7381 gccaccgtca ccggcgattt gatgcggccg cgcccgtcgc agtgcgggca ggagcgcagc
     7441 aactggtttt ccagactctc gcgggtgcgc tggcgggtca tctcgaccaa acccagctcg
     7501 gaaattttta agatattggt gcgcgctttg tcttttttca gcgcttcctt gagcgcgtcg
     7561 tagaccttct tgcggttggc ctcgcgctcc atgtcgatga agtcgatgat gatcaaaccg
     7621 ccggcgtttc tcaggcgcaa ctggcgcacc acttcctgca ccgcctcgag attggtcttg
     7681 agaatcgtct cttcttgact gcgcttgccg acgaaacggc cggtgttgac gtccaccgcg
     7741 gtcagcgctt cggtccgctc gatgatgata taaccgcccg agcgcagcca ggcgcgcggg
     7801 tcgagcgcct tggtgatctt ttcttcgatg ccgagctgct cgaacagcgg ctccttgtcg
     7861 gaatagagat tgatcttcga cttcaagcgc ggcatgaagt ggcgggcgaa atcgacgatg
     7921 cgccgatgat ccttgggcga gtcgatcaac acctgctcgg tatcggtggt gaaaaaatca
     7981 cgaattgccc gtgcgataag atcgagatcc tgatgaatca acgacggcga cggcgcgctt
     8041 tcggcttttt tctgaatgct tttccacagc ttctgcaaaa accctaggtc atgctgaatc
     8101 tcgcgcttgc tcagcccctc gcaggcggtg cgcaggataa agcccccttc ttctgtgccc
     8161 aaagactggg cgatttcctt gagccgtttg cgctcttcgt cgctctcgat acgccgcgag
     8221 atgccgatat gcttgttgcc cggcatgaag accatgtagc gtcccggcag ggaaatatgc
     8281 gaggtcaccc gcgcgccctt ggtgccgagg ggatccttgg cgacttgcac gagcacttct
     8341 tcaccgcgcg a
//
LOCUS       DASQKS010000154         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_504203, whole genome shotgun sequence.
ACCESSION   DASQKS010000154 DASQKS010000000
VERSION     DASQKS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504203"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VE170_03930"
     CDS             <1..609
                     /locus_tag="VE170_03930"
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HYJ14613.1"
                     /translation="ACQVLHCCATIETEEQLRQNSTTFVPFSVDALSLYDVKASHEQA
                     WCYAQLREHSVNNVLADLSILNSSGEVVAQLEGFRLRPITQQGLEALLQRATTHGDGN
                     WIPGSLPGPMDDIDKAALTVQQVVRYLQAQCAELSGYPESAICSDQGFMAMGLDSLVA
                     MILANRIRQDFNCAVSATQILLSVSIESLAQVICVRSSETGF"
     gene            complement(761..1750)
                     /locus_tag="VE170_03935"
     CDS             complement(761..1750)
                     /locus_tag="VE170_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14614.1"
                     /translation="MLIRKIVLVALLISIGGLTHPAFCATTQTSMRLLYPSFAGSWAT
                     AWIAKEAGYFSAEGLDVELVRVGGSSRMVAAMLGGSAPIIQAGAIAALTANVAGADVV
                     IIGSTGTVSPFRLIARPEIKQPSDLKGKKAGITTFGSTSDQVLRIALKQFNLDPSKDV
                     ALLTFGAQPEAFAALMNGIVQVAALSYPLYPKAIKSGMRELVNFADLGVEDINGTVMT
                     TRAFVAQQRDMALRFMRAFTRGMQRYRTDKEYSKKVLAKYGKINDEEILEGTWQDYAP
                     TLQKTPRPSLKAVQFMIENQFAGKKPLPKPEQFVDSSIVDQLEKSGFFDSFNK"
     gene            1828..3084
                     /locus_tag="VE170_03940"
     CDS             1828..3084
                     /locus_tag="VE170_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14615.1"
                     /translation="MQRDDTSKTMVNKSAGAEIAAPMAPSGASFGLQAAPVFFLTAIF
                     FLNFIARVILSPLLPTIEKELAIGHGQAGFFFFLISAGYLSGLLGSGFLSSRTSHRQT
                     ILLSGAGVGLALFLLSGSSSLWAMRLGLYALGLAAGLYMPSAIATITALVDQRHWGKA
                     IAVHELAPNLAFFTGPFVAELFLRHSTWRAALAVLAGASLLITMGYYRYGQGGRFAGE
                     SPASSAFGVLVRTSAFWIMLILFGMGVSSTVGIYAMLPLYLISDRQIEATWANTVTAL
                     SRSYGPVFGMLGGWASDRLGPKRTMVVSLIVTGVLTLFLGFVADNWISAVVIIQPALA
                     VWFFPAGFAALAVIAPPQARNLAVGFTIPFGYLIGGGAIPTFIGMMGDAGLFALGFAI
                     TGLLITGAGVLALSLKLPRTIQGGSH"
     gene            3129..3881
                     /locus_tag="VE170_03945"
     CDS             3129..3881
                     /locus_tag="VE170_03945"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HYJ14616.1"
                     /translation="MKLDEFAAKIRESDGIVVFTGAGISTESGIPDFRSPGGIWTRYR
                     PVTFQEYMTSESARIEAWKRRLDGWEQYKNAKPNVGHHFIQALSLKEKLIGLITQNVD
                     GLHSMAGLADDKIVELHGSNRKILCMGCAKEFAPEPIVNSLIGDFASPRCDSCGGILK
                     SATVSFGQAMPEQAMRIAQEWTEQAEVFIVMGSSLQVQPAASFPVVAKRNGALLAIVN
                     REEGPLDGLADFVHNGSIGEFCGKFGEMIADG"
BASE COUNT          792 a         1130 c         1199 g          935 t
ORIGIN      
        1 gcttgccagg tgctgcactg ttgcgccacc atcgagaccg aggagcaact gcgacaaaac
       61 tcgacaacct ttgtaccgtt ttcagtggac gcgctgtcgc tgtacgacgt caaagcgagc
      121 cacgagcagg cttggtgcta cgcccaactt cgcgagcata gcgtaaacaa cgtgcttgcc
      181 gatttgtcga ttttgaacag ctccggggaa gtggtcgcgc aattggaggg tttccgtctg
      241 cggccgatta cccagcaggg attagaagcc ttgctgcagc gagcaaccac gcatggcgat
      301 ggcaattgga tacccggctc tcttcccgga cccatggacg acatagataa agcggcgctg
      361 accgtgcaac aagtcgtgcg ctatctgcaa gcgcagtgtg cggaactttc aggctatccc
      421 gaatcggcga tatgcagtga tcaaggtttc atggcgatgg ggctggactc cctggttgcg
      481 atgattctag ccaaccgtat ccgtcaagac ttcaactgcg cggtttccgc cacgcagatt
      541 ttgctgtccg tgagtataga atcactcgct caagtcatct gcgtaagaag ctctgaaacg
      601 ggtttctgat aattcaaatg ctgcactgac tgcgccaatg gctgtgtggg ttgcaaacca
      661 acgcgcaaga ctgagcatca agctttcggt gtcgtccatg gttgacgatg gatcaaattc
      721 aaggggtggc ccgacgcatt gcgtcgcgct ctccggttat tcatttatta aatgaatcga
      781 aaaagccgct cttttccagt tgatcgacaa tcgaagagtc gacgaactgt tcgggttttg
      841 gcagcggctt ttttccggca aattggttct caatcataaa ctgaaccgct ttgagcgacg
      901 ggcgcggtgt tttctgcaac gtcggcgcat aatcttgcca ggtgccttcc aagatttcct
      961 cgtcattgat cttgccatac ttggccaaaa cttttttgct gtattcttta tcggtgcgat
     1021 agcgctgcat gccgcgcgtg aaggcgcgca tgaagcgtag tgccatgtcg cgttgctggg
     1081 cgacgaaagc gcgcgtcgtc atcacggtgc cgttgatgtc ctcaacaccg agatcggcga
     1141 aattgaccag ctcgcgcatg ccgcttttga tcgcctttgg atagagtgga tagctgaggg
     1201 cagcgacctg cacgatgcca ttcatcaacg cagcgaacgc ctccggttgc gcgccgaagg
     1261 tgagcagcgc gacatctttg ctcgggtcga gattgaactg tttgagcgcg atccgcagga
     1321 cttgatcgga agtcgagcca aaagtcgtga tgccggcttt tttgcctttc aagtcggacg
     1381 gctgcttgat ctcggggcgc gctatgagac ggaagggcga gacggtaccc gtcgaaccga
     1441 tgatcacgac gtcggcgccg gcgacattgg cggtgagcgc cgcaatggcg ccggcctgaa
     1501 tgatcggcgc gctgccgccg agcatggccg caaccattct gctgctgccg ccgacgcgga
     1561 cgagttcgac gtcgagccct tcggcactga agtatccggc ctccttggcg atccacgccg
     1621 tcgcccaact gccggcgaag ctcgggtaga gcaagcgcat ggatgtctga gtggtcgcgc
     1681 aaaatgccgg atgcgtcagg ccgccgatac ttatgagcag ggcgaccaga acaatttttc
     1741 taatcaacat ggtccctcct ggatttgtcg atacctggcg ttggcttctt cggccaaccc
     1801 attgtagaca ttgtctgatt tacgaagatg cagcgcgacg atacctcaaa gacgatggtg
     1861 aataaaagcg ccggcgcgga aattgccgcg ccgatggccc catccggcgc gtcgtttggg
     1921 ctgcaggcag cgccggtatt ctttttaacg gcgatttttt ttctcaactt catcgcccgg
     1981 gtgattctct cgccgctgtt accgacgatt gaaaaggagc tggcgatcgg tcacggccag
     2041 gcgggctttt ttttcttctt gatctccgct ggttatcttt cgggtttgct cggatcgggg
     2101 tttctctcgt cgcgcacaag ccatcgccag acgattctcc tctcgggcgc cggtgtcggt
     2161 ttggcgctgt tcctgctttc cgggtcgagc agcctgtggg cgatgcggct cgggttatat
     2221 gccttgggat tggccgccgg actctacatg ccctcggcga tcgcgacgat caccgcgttg
     2281 gtcgaccaac gccattgggg caaagcgatc gcggttcacg aactggcgcc caatctggcg
     2341 ttttttaccg gtccgttcgt tgctgaactt tttctccgcc actcgacttg gcgcgcggcc
     2401 ctggcggttt tggctggcgc ctcgctgctg atcaccatgg gctactatcg ttacggccag
     2461 ggcggccgct tcgccggcga atcgccggcg tcgagtgcct tcggcgtgct ggtgcgcaca
     2521 tcggcgtttt ggatcatgtt gattcttttc ggcatggggg tcagttcgac cgtcgggatt
     2581 tatgccatgt tgccgctcta tctgatcagc gatcgccaga tcgaggcgac ctgggcaaac
     2641 acggtaaccg cgttgtcgcg ctcctacggg ccggtgttcg gcatgctcgg cggctgggct
     2701 tcggaccgcc tcgggccgaa acggaccatg gtggtgagtt tgatcgtcac cggcgtgctg
     2761 actttgtttt tggggttcgt ggccgacaat tggatcagcg ctgtggtgat catccaaccc
     2821 gccctcgccg tatggttttt tccggccggc ttcgcggcgc tagcagtgat cgcaccgcca
     2881 caagcgcgca acctcgcggt tggctttacc attcccttcg gctatttgat tggcggcggg
     2941 gcgatcccga cgtttatcgg catgatgggc gacgccggac tttttgcgct gggtttcgcc
     3001 attaccggac tcttgatcac tggcgccggc gttctcgcgc tgtcgctcaa gctgccgcgc
     3061 acgatccaag gcggatccca ctagttttcg gttgtcagcg gcgcaccggc tgttctataa
     3121 accgtaatat gaagctggac gagttcgctg cgaagattcg cgagagcgat gggatcgtgg
     3181 tttttaccgg cgcagggatc agcaccgagt ccggcattcc cgacttccgc agtcccggcg
     3241 gcatctggac gcgttaccgt ccggtgacgt ttcaagaata catgaccagc gagtcggcgc
     3301 gcatcgaggc ctggaagcgc cgtctagacg gctgggaaca atataaaaat gctaaaccca
     3361 atgtcggcca tcattttatc caagcgctaa gcttgaaaga aaaattgatc ggcctgatca
     3421 cgcaaaatgt cgacggcctg cacagcatgg ccggcctcgc cgacgataag atcgtcgaac
     3481 tgcacggctc gaaccgaaaa atcctttgca tgggctgcgc caaggaattc gcgccggagc
     3541 cgatcgttaa cagtttaatc ggcgacttcg cctcgcccag gtgcgacagc tgcggcggca
     3601 tcctcaagtc ggctacggtc tccttcggcc aagcgatgcc cgagcaggcg atgcgtatcg
     3661 cacaggaatg gaccgagcaa gcggaagttt tcatcgtcat gggctcatcg ttgcaggtgc
     3721 agcccgccgc gtcctttccg gtagtggcga aacgcaatgg cgcgttgttg gcgattgtca
     3781 atcgcgagga aggtcccttg gatggcttgg cggacttcgt ccataacggt tcgatcggcg
     3841 aattctgcgg caaattcggc gagatgatcg ccgatggctg acgcggatgc ggagtgttgg
     3901 ccgcaaagaa cgcaaaaggc gcaaagttaa aacactaaaa ttctttacga aaaacacgaa
     3961 ggtcgaaaaa caattatccg aacccttcgt gcccttcacg gtgaaaacga ccgaaacttc
     4021 ctgacgacga aagggtaaat tcatggctga ctggaa
//
LOCUS       DASQKS010000155         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2618506, whole genome shotgun sequence.
ACCESSION   DASQKS010000155 DASQKS010000000
VERSION     DASQKS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618506"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..261)
                     /locus_tag="VE170_03950"
     CDS             complement(70..261)
                     /locus_tag="VE170_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14617.1"
                     /translation="MFIYLKVGLSFVLVLFGVKMMLVDVYKIPIGASLVATGGILLLS
                     VLASLSQRRTFDSSVDDAS"
     gene            complement(322..1599)
                     /locus_tag="VE170_03955"
     CDS             complement(322..1599)
                     /locus_tag="VE170_03955"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ14618.1"
                     /translation="MLPREENDMLTLVSPDKPMGQLMRRYWIPALLASEIPEPDCPPV
                     RVRLLGEDLVAFRDTQGRIGLLEEHCAHRGTSLFFGRNEECGLRCIYHGWKYDIDGKV
                     LDTPAEPAGSTLKDKVRQVAYPCREAGGMIWTYMGAPDKQPLLPNYEWMTLAPDNLYV
                     TRSIQDCSWLQGLEGECDSSHLSFLHRSFTGDRPRGGGDGNLYAADTAPTLEGIEMDY
                     GVRMLSCRKIGPDSIYLRVSNIVMPCHGFIPTGGIKGNPEGYTIHSHVPVDDTHSMRY
                     NIHFRRNRAIEPEERQHDDEIGPDFKKIRNLQNDYLIDRDKQRRENFTGMGPIFLNHD
                     ACATETRGPIYDRSQEHLGVSDMTVIAVRKFLLNAARAVAAGKEPPHIIRTAAQTDVR
                     HVACIATTIPSSRDPKTYVVEQLKKDKYWEAEN"
     gene            complement(1631..1855)
                     /locus_tag="VE170_03960"
                     /pseudo
     CDS             complement(1631..1855)
                     /locus_tag="VE170_03960"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
     gene            complement(1901..2785)
                     /locus_tag="VE170_03965"
     CDS             complement(1901..2785)
                     /locus_tag="VE170_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ14619.1"
                     /translation="MALTAQENFDLTRVGPGTPCGILMRRYWHPVGFSAELKGKPVRR
                     MLLGEDLVLFRDEQGRLGLIGARCPHRGTSLHLGYIEDGGLRCCYHGWLFDVSGRCLE
                     QPTEPPEANFKDKMRQPSYKVEELAGVIFAYLGPEPAPLLPRYDVLVREDGIRSLQGR
                     IASCNYLQMVENTVDQHHFKWLHRTPNTKKWTDPVLTSEYFDYGIRDTFARTGGGERY
                     QTLSYFIMPTMNKTGYHVPESHPCGMSANHPGYEALRWRVPADDTHTMHFTVYFAPVV
                     DGKPTGTIPPDNAAKGVV"
     gene            complement(2960..4024)
                     /locus_tag="VE170_03970"
     CDS             complement(2960..4024)
                     /locus_tag="VE170_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14620.1"
                     /translation="MISIRNLLKIYKSGSDAVHAVDDVSVDVGDQEFFVLLGLSGSGK
                     TTLLRCVAGLERPDGGEIAISNVLVSSAARNFFVAPEGRGIGMVFQSYAVWPHMTVYD
                     NIAFPLINGPLKLSRADIKKRVLHALELVQLGALADRPAPFLSGGQQQRVALARALAV
                     EPKVLLMDEPLSNLDARLREEVRDEIKNLSKKFGIAVLYVTHDQVEAMALADRVAVMS
                     KGKVLQCAPPRELYDRPSSREVCEFLGSTNLIDGVVGNGGHVDTDLGKLACAIGDKNL
                     RNVSVAIRPEELQMAAAPTGLENEFAAEVLASTFLGELTVCDVTVNGKALRFKTTQST
                     APAQRVYIRLPAEKLKLFAR"
BASE COUNT          738 a         1152 c         1280 g          866 t
ORIGIN      
        1 gcccgtttcg gctgaacttt gagctactac aattgtgttt gcaggggatt cataatgttg
       61 ctcgatggct taggacgcgt cgtcgacgct gctgtcgaat gtgcggcgct gcgacagtga
      121 cgccagcacc gagagcaata aaatcccgcc agtcgccacg agcgatgcgc cgatggggat
      181 tttgtagacg tcgaccagca tcattttaac cccaaataaa accagcacga aagaaagtcc
      241 gactttcaaa tagatgaaca tttcatcacg cccgcgagcg tgaaatagag cacgaaccga
      301 ggatggcgag cacgtttgcg gctaattttc cgcttcccag tacttatctt tcttcaactg
      361 ctcgacgaca taggtcttgg gatctcggct cgaggggata gtcgtggcga tgcaggcaac
      421 gtggcggacg tcggtttggg cggcggtacg gatgatgtgc gggggttcct tgccggcggc
      481 gacggcgcgg gcggcgttga gcaggaattt gcgcacggcg atgaccgtca tgtcgctgac
      541 acccaggtgc tcttgagaac ggtcgtagat cggtcccctg gtttcggtgg cgcaggcgtc
      601 gtggtttaga aagatcggtc ccatgccggt gaagttttcc cgccgctgct tgtcgcggtc
      661 gatcagatag tcgttttgca ggttgcgaat ttttttgaaa tcgggaccga tctcgtcgtc
      721 gtgttgtctt tcttccggct cgatcgcccg gttgcggcgg aagtggatgt tgtagcgcat
      781 gctgtgggtg tcatcgaccg gcacatggga gtggatcgtg taaccttccg ggttgccttt
      841 gatgccgccg gtggggatga agccgtggca cggcatgacg atgttcgaca cgcgcagata
      901 gatcgagtcc gggccgatct tgcggcagga caacatgcgc acgccgtagt ccatctcgat
      961 gccttccagc gtcggcgcgg tgtctgccgc gtaaagattg ccgtcgccgc cgccgcgcgg
     1021 ccggtcgccg gtaaaagatc ggtgcaggaa cgacaggtgc gacgagtcgc attccccttc
     1081 gagcccctgg agccaactac agtcctggat cgaccgggtg acgtagagat tgtccggcgc
     1141 cagggtcatc cactcgtagt tgggcaagag cggctgcttg tccggcgcgc ccatgtaggt
     1201 ccaaatcatg ccgccggctt cccggcacgg gtaggcgacc tggcgcacct tgtctttgag
     1261 ggtgctgccg gcgggctccg ccggcgtgtc gagcaccttg ccgtcgatgt catatttcca
     1321 gccatgatag atgcagcgca gaccgcactc ttcattcctg ccaaaaaaca gcgatgtgcc
     1381 gcgatgggcg cagtgttctt cgagcaggcc gatgcggcct tgagtatcgc gaaaggcgac
     1441 tagatcttcg ccgagcaagc gcacgcgcac gggcgggcag tcgggctcag ggatttctga
     1501 agcgagcagc gccggtatcc aatagcggcg catcagttgg cccatcggct tgtccgggct
     1561 caccagggta agcatgtcgt tttcttcgcg tggcagcatg gcctggtcct cttgattgcc
     1621 gagagtcgat tcagccgatt ttgaattctc ccggcgcgat ttcaacgatg cggtttttcg
     1681 ccgggtcgcg aatcgtgccg atgggatctt ggccggtggc gatggcgtcc atggagtctt
     1741 tgtaaagctt gcgcagcatg atcacgcctt tgtccgatac gcccaggtgt tcgatagtgc
     1801 ggtcgcaaat caccccttgg ctttcttggg cggcgcggtc ctggtcgccg cgcgcgagat
     1861 agccggtcgc ttcgtcgtag cggtactgcc ccggcgtagc ttagaccacg cctttggcgg
     1921 cgttatccgg cgggatagtg ccggtcggtt tgccgtcgac caccggtgcg aagtagacgg
     1981 tgaaatgcat cgtgtgggta tcgtcggcgg gcacgcgcca acgcaacgct tcgtaacccg
     2041 gatgattggc cgacatgccg cagggatggc tttcgggaac gtgatagccg gtcttgttca
     2101 ttgtcggcat gatgaagtag ctgagcgtct gatagcgttc accgccgccg gtgcgcgcga
     2161 aggtgtcacg aataccgtaa tcgaagtact ccgaggtcag caccggatcg gtccattttt
     2221 tggtattggg cgtgcggtgc agccacttga agtgatgttg atcgacggtg ttctcgacca
     2281 tctgtaaata attgcagctg gcgatgcgtc cttgcagcga acggatcccg tcctcgcgca
     2341 ccaacacgtc atagcgcggc aatagcggcg ccggctcggg acccaggtag gcaaagatga
     2401 cgccggcgag ctcttcgact ttgtacgaag gctggcgcat tttgtccttg aagttggctt
     2461 ccggcggttc cgtgggttgt tccaaacaac ggccgctcac atcgaacagc cagccatgat
     2521 aacagcagcg cagtccgccg tcttcgatgt aacccaggtg gagcgacgtg ccgcgatgcg
     2581 gacagcgggc gccgatcagg ccgaggcgcc cttgctcgtc acggaataaa accagatctt
     2641 cgccgagcag catgcgccgc accggtttgc ctttgagttc ggcgctaaaa ccgaccggat
     2701 gccagtaacg ccgcatcagg atgccacatg gcgtaccggg gccgacccgg gtcaggtcga
     2761 agttctcttg ggcggtgagt gccattttga cgttctccta cgctacgcga agttttgccg
     2821 ctatcgggaa aatacctttt acgacgaagg agaggaagag cacgaagctt tggtgatagg
     2881 acgccacaag accggccgcg gcctctgtgc attctttgcg gccaatcgcc gcttttctcc
     2941 agcgtgcctc gctcttcagc taccttgcga acagttttag tttttcagcc ggcagccgga
     3001 tgtaaactcg ctgcgccggc gcggtgctct gggtggtttt gaaccgtaag gccttgccgt
     3061 tcaccgtcac gtcgcacacc gtcagttcgc caagaaaggt cgacgccagt acttcggcgg
     3121 caaattcatt ttccaatcca gtgggcgcgg ccgccatctg cagctcttcc ggccggatgg
     3181 cgacgctgac gttgcgcaaa tttttatcgc cgatggcgca ggccagtttt cccaaatcgg
     3241 tgtcgacgtg accgccgttt ccgacaacgc cgtcaatcaa gttggtcgag ccgagaaact
     3301 cacagacttc gcggctcgac ggccggtcgt aaagctcccg cggcggcgcg cactgcagga
     3361 ccttgccctt gctcatgacc gcgacccgat cggccagcgc catggcttcg acctggtcgt
     3421 gggtgacgta gagcacggcg atgccgaatt ttttcgagag atttttgatc tcgtcgcgca
     3481 cttcttcgcg caggcgggcg tcgagattgc tgagcggctc gtccatcaac agcactttcg
     3541 gttcgacggc gagggcgcgg gcgagggcga cgcgctgctg ctggccgccg ctcaaaaacg
     3601 gcgcgggacg gtcggcgaga gcgccgagct gcaccaactc gagggcgtgc agcacgcgct
     3661 tcttgatgtc ggcgcgggat aacttgagcg gtccgttgat cagcgggaag gcgatattgt
     3721 cgtaaaccgt catatgcggc cagacggcgt aggactgaaa caccatgccg atgccgcgtc
     3781 cttcgggagc gacgaagaaa ttgcgagcgg cggagctgac caggacgttg ctaatggcga
     3841 tctcgccgcc gtcgggccgc tccaagccgg cgacgcagcg caacagcgtg gtcttacccg
     3901 agccgctgag cccgagcagg acgaagaact cctggtcgcc gacatcgacg ctgacgtcgt
     3961 cgacggcatg gaccgcgtcg ctgccggact tgtatatctt cagtaggttt cttatcgaaa
     4021 tcattggtcg cgctta
//
LOCUS       DASQKS010000156         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5544412, whole genome shotgun sequence.
ACCESSION   DASQKS010000156 DASQKS010000000
VERSION     DASQKS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5544412"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VE170_03975"
     CDS             <1..847
                     /locus_tag="VE170_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ14621.1"
                     /translation="NDALYGLMRVGFSAARVLPMHVPLRLLGGLAPHIFIRSARRARR
                     QLREILPDLDPVRVTRRMFVHFAESLWELSRLHRSVPELDASSRQVLDAVLAEGKGAM
                     VISGHIGNWELLGHAIAAAGYPIATIAKPSYDPRITQWLQQWRSSRGLQIVWSDEGNA
                     GKMILSVLRRNRLMAFLIDQNTKTAGNFVPFFSRPAFTSTTPAAIALRTGAPIIFCWH
                     HRRGKRHQMSFERISYAPTGDRTGDILKLTAIFNDRLEHAIRQAPEQWVWLHARWGRS
                     RGNLS"
     gene            complement(979..2850)
                     /gene="glsA"
                     /locus_tag="VE170_03980"
     CDS             complement(979..2850)
                     /gene="glsA"
                     /locus_tag="VE170_03980"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HYJ14622.1"
                     /translation="MQQVSATQIDIEATSPSPLMTALQAIHEQYADNFSGKVATYIPE
                     LAKANPKLFGISLVTADGYIYEVGDAGHLFSLQSISKPFVYGLALEDHGEDYVLTKVG
                     VEPTGEAFNSIVFDERHNRPFNPMVNAGAIATTALIKGESPERRLSRVLTKFNDLAGR
                     SLAIDHDVYLSESRSGHRNRAIGYLELNAGMIDAQVDEHLDLYFKQCSILASARDLAV
                     MAATLANDGINPLRGKRALQAGYVKNVLSVMHSCGMYDYTGEWTYRIGLPAKSGVSGG
                     IIAVLPGQFGIGIFSPLLDDQGNSCRGIQVCEELSARFKLHMFGARAAAGVGLRRHYR
                     ATTVRSIRQRGSIEQAILDRKGDAICVYELQGNLFFGALEQVFRKLTTELATLTYLVI
                     DVKRVLEIDQCAMTLIVQLNGWLAKQGKKLIFAHLAPRFADRLKRHPDYVWTDKSFFR
                     DTETALEWCENRVLAQEQPRRVADNLQVPLSAINILAGFDAQETALVESIVEEVHYAQ
                     GETIIREDDRADALFLLAAGTVTVCLRLTDGNRQRRLSTITPGLAFGELALLDGGKRS
                     ADVVADEPALCYVLPIAKFRELASNHPGIETKLIFNICRELSARLRRADAEIRSLAE"
     gene            complement(3099..3905)
                     /locus_tag="VE170_03985"
     CDS             complement(3099..3905)
                     /locus_tag="VE170_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYJ14623.1"
                     /translation="MKFIAAAVQMVATDDKAANLAEAEHWLREAVHQGARLAVLPEVF
                     IWRGSKKFERVFAEPIPGPTSDKLAGLARELKIHLVGGSILEEIPANPKAYNTSLLFD
                     PSGSLIAAYRKIHLFDVDLANGASLRESETRAHGGDVVVAQTELGALGLSVCYDLRFP
                     ELYRRLADRGAQLIFAPSAFTAFTGRAHWETLVRARAIENQAYVIAADQFGKSAKSFE
                     CHGHSMIVDPWGTILAELADGPGVVMAEIDLDRLANVRAELPALEHRKLL"
     gene            complement(3944..>4402)
                     /locus_tag="VE170_03990"
     CDS             complement(3944..>4402)
                     /locus_tag="VE170_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ14624.1"
                     /translation="GFTAGMLGSTAETIIRKCPRPIFVSTERFTSIANPLLAYDGSQR
                     ASSAMESAAEFCAVLKLPLTVLTIGREETAAQAISQQAKAYLGSYAIDVKYDIGRGYP
                     EQKIIEYLVNLKYDLLFIGAHGHRRIIKMVLGSTTEYVLRNAPCPVFLNR"
BASE COUNT          948 a         1264 c         1235 g          955 t
ORIGIN      
        1 aaatgacgcg ttatacggct taatgcgggt cggtttcagt gcggcgcgag tcttgccgat
       61 gcacgtcccc ttgcgtttgc tgggtggtct ggcgccccat atttttatcc gatctgcgcg
      121 gcgggcgcgt cgtcaactgc gtgagatttt gccggatctc gacccggtca gggtcacgcg
      181 gcgcatgttc gttcatttcg ccgaatctct ctgggagctt tcacgtttac atcgatcggt
      241 gccggagctg gacgccagct cccggcaagt ccttgacgcc gttctagccg aaggcaaagg
      301 cgccatggtg atttccggcc atattggcaa ttgggaactc ctcggccatg ccatcgccgc
      361 tgccggctat ccgatcgcaa ccatcgccaa gccatcgtac gatccacgga taacgcaatg
      421 gctgcaacaa tggcgcagca gccgaggcct gcagatcgtc tggagcgacg aaggcaacgc
      481 cgggaaaatg atcttgagcg ttctgcggcg caaccgtctg atggcatttt tgatcgacca
      541 gaacacaaaa acggccggca attttgtccc gttcttcagc cggccggctt tcacttcaac
      601 gacacccgcg gcgattgcct tgcgtaccgg cgcgccgatt atcttctgct ggcatcaccg
      661 tcgcggcaaa cgccatcaaa tgagttttga gcggatatcc tatgcgccga ccggcgaccg
      721 aaccggggat atattgaaat taaccgccat tttcaacgac cgtttggaac acgccattcg
      781 tcaagctccc gaacaatggg tgtggctcca cgcgcgctgg ggccgcagtc ggggcaacct
      841 tagctaacga ttgcctcacg gtgtcgcagt tacaacctgg gcgaccgcgc tgcgagatcg
      901 gcttgatcca gcgccatcac tggccgggca cgctctgtac tcatggctta tttctaaccg
      961 ccgttcggcc actccgtgct attccgccag tgagcgaatt tccgcgtccg cgcgccgcag
     1021 gcgggcggag agttcacggc agatattgaa aatgagtttg gtctcgatcc ccggatggtt
     1081 gctggcgagc tctctgaact tggcgatcgg cagcacgtag cacagcgccg gctcgtcggc
     1141 gaccacatcg gcggaacgct tgccaccgtc caataaggca agttcaccga aggctaagcc
     1201 cggcgtgatc gtgctcagcc gccgctgtcg attgccatcg gtgagtctaa ggcagacagt
     1261 taccgttcca gccgcgagga gaaaaagcgc gtctgcccga tcgtcttcac gaatgatcgt
     1321 ttcgccttgg gcatagtgaa cctcctcgac gatagattcg accaaggcgg tttcttgagc
     1381 gtcgaaaccg gccagaatat taatcgcgct gagcggtact tggagattgt cggccactct
     1441 gcgcggctgc tcttgcgcaa gcacgcgatt ttcgcaccac tccagagcgg tctcggtatc
     1501 gcgaaaaaaa cttttgtcgg tccagacata atcaggatgt cgtttaagcc tatcggcgaa
     1561 gcgcggcgcg agatgggcga agatgagctt cttgccttgc ttggccaacc agccgttcaa
     1621 ctggacgatc aatgtcatgg cgcattgatc gatttccaac acgcgtttaa cgtcgatgac
     1681 gaggtaggta agcgttgcca attccgtggt gagcttgcga aacacctgtt ccaaggcgcc
     1741 gaaaaataga tttccctgga gttcataaac acaaatcgcg tcccccttgc gatcgaggat
     1801 cgcctgctcg atgctgcccc tttggcggat cgatcgcacg gtggttgccc gataatgccg
     1861 gcgcaggcca accccggctg ccgcgcgggc gccgaacatg tgcagcttga agcgcgccga
     1921 aagctcttcg caaacctgaa tcccacggca actgttgcct tgatcgtcga gcaatggcga
     1981 gaatattcca atgccaaact gaccgggaag aacggcgatg atgccgccgc tgacaccgct
     2041 cttggccggc aaaccgatgc gataggtcca ctctccggta taatcgtaca tcccgcagct
     2101 gtgcattacg ctcaaaacgt tctttacata gcccgcttgc agcgcccgct tgccgcgcaa
     2161 cggattgatt ccgtcgttcg ccagagttgc cgccattacg gcgaggtccc gcgcgctggc
     2221 caaaatcgag cactgcttaa agtacaaatc gagatgctcg tcaacttgcg cgtcgatcat
     2281 gccggcgttg agttccagat agccgatggc gcggttacga tgtccgctcc gggattccga
     2341 gagataaacg tcgtgatcta ttgccaaaga acgaccggcc aagtcgttaa attttgtcag
     2401 taccctggac agacgacgct cgggagactc gcctttgatc aaagcagttg tggcaatggc
     2461 tccggcattg accatcggat tgaaagggcg gttgtggcgc tcatcgaaca cgatggaatt
     2521 aaaggcttcg cccgtcggct cgaccccgac cttggtcaga acgtaatcct caccgtgatc
     2581 ttctaacgct aaaccataga caaagggttt cgagatcgat tgaagcgaaa ataaatggcc
     2641 ggcatcgccg acttcgtaga tatagccatc ggcggtgacc agcgaaatgc caaacagttt
     2701 tgggttggcc ttggctaatt cagggatata ggtcgcgact ttaccgctaa aattatcggc
     2761 gtattgctca tgaatagctt gcaatgcagt catcagcggc gatggactgg tcgcctcgat
     2821 gtcgatttgc gtcgctgaaa cttgctgcat ctggaaatcg caatagtgct tttagccgac
     2881 gtggacaatt ggctacgttg aaccaaggta gcgctgaact agaaaatccg gccttcagga
     2941 ttttgcgtgc actctgcatg ccagcgggcc gcaacttttg tccataacta accgaccaat
     3001 ttcgtgggga ttccatccga tgcttcaggc gcgcaaccat caactgcgcg gtttgatgat
     3061 ttcttaagca caaccctgat gattatttag gcgtggcatc agagcagctt ccggtgctcc
     3121 aacgcgggta gctcggcgcg cacgttggcg aggcggtcga gatcgatctc ggccataacc
     3181 acgcccgggc catcggctaa ctcagcgagg atagtgcccc agggatcgac gatcattgag
     3241 tggccgtggc actcgaaact tttggcgctt ttgccgaatt gatcggcggc gatcacataa
     3301 gcttgatttt caatggcgcg ggctcgcacc agagtctccc agtgcgctcg accggtaaag
     3361 gccgtgaatg ccgagggagc gaagatcagc tgcgcgcccc ggtcggccaa tctacgatag
     3421 agctctggaa agcgtaaatc gtaacacacc gataagccca gcgccccaag ctcggtctgc
     3481 gcaaccacaa cgtcgccgcc atgcgcgcgg gtctccgact cgcgcagcga cgcaccgttg
     3541 gccaagtcga catcgaagag atgaatcttg cggtaggctg caatgaggct tcccgaagga
     3601 tcgaagagca ggctggtgtt gtaggctttt gggttagccg gaatttcttc aaggatcgaa
     3661 ccgccgacta aatgaatttt taattcacgc gccagcccgg caagtttatc ggaagtcggt
     3721 ccaggaatcg gctcggcaaa gacgcgctca aatttcttgc tgccgcgcca gataaagact
     3781 tccggcagga ccgccagccg cgcgccttga tgtactgcct cgcgcagcca atgctcggct
     3841 tcagccagat ttgccgcttt gtcatcggtc gccaccatct gcaccgccgc ggcaatgaac
     3901 ttcatagctc cgataccttg aacgcttata cacctgaaag ctgttatcga ttcagaaaaa
     3961 ccggacaagg cgcattgcgc aggacatact cagtggtgct gccgagcacc atctttataa
     4021 tccgccgatg accgtgagct ccaatgaaca gcaggtcgta tttgagattg accagatact
     4081 cgatgatttt ttgttccgga tagccgcgcc cgatgtcata tttaacgtcg atggcatagg
     4141 aaccgagata ggccttagcc tgttgcgaaa ttgcctgtgc cgccgtttct tcgcggccga
     4201 tggtcagcac ggtgagcggc agcttgagca cggcgcaaaa ttccgccgcc gattccatcg
     4261 ccgaactcgc tcgctggctg ccgtcatagg ccaatagcgg gttggcgatg ctggtgaagc
     4321 gctcggtcga aacaaagatc ggccgggggc atttgcggat gatggtctcc gccgtagaac
     4381 cgagcatgcc cgcggtgaag cc
//
LOCUS       DASQKS010000157         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5976653, whole genome shotgun sequence.
ACCESSION   DASQKS010000157 DASQKS010000000
VERSION     DASQKS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5976653"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..787)
                     /locus_tag="VE170_03995"
     CDS             complement(383..787)
                     /locus_tag="VE170_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ14625.1"
                     /translation="MVKGTDVWCVEPDATVFDALRRMAEKEVGALVVTEGAQVVGLIS
                     EREYARKVVLQGRTSPTTLVKEIMISPVVYIHRDQAIEECMSLITEKRTRHLPVIEDG
                     NLIGLISIGDIVKSIIADQRFLIEQLVRYVSG"
     gene            complement(848..1075)
                     /locus_tag="VE170_04000"
     CDS             complement(848..1075)
                     /locus_tag="VE170_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14626.1"
                     /translation="MKTKTNNSAEIFIGFTLLAKKFTPFDRAELECRTGAYPGRQTES
                     IPFSRIAFIPNSALAASYFAANMDLRAHLVV"
     gene            complement(1296..>2143)
                     /locus_tag="VE170_04005"
     CDS             complement(1296..>2143)
                     /locus_tag="VE170_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14627.1"
                     /translation="PGNPTIVVENMTGAGGFIQANFMFQRAKADGLTVGSNQGSFILQ
                     QILGARGIEFDSRRFEYLGVPTEFHPVCALTKASGVTSMERWFVAKEPVKLGGIGPGT
                     GPSDIARTLKAALPTLPVRVIDGYKGAADVRLAADAGELAGFCAAWEGIKLLWRKSID
                     SGEVSVVLQVAPKRHSELPNVPLAIDFAKSDEAKQILKYAVQDVAALQYLYFLPPATN
                     RDLVRMLRKSFLDTLKDPEFLTEMNKANLAVTPVGGETIEGIVAGLFKLDATMIAKLK
                     SVLVP"
BASE COUNT          432 a          592 c          600 g          519 t
ORIGIN      
        1 catcgactgc tggattccat tgataaaaac caacgtttct tctgcttcgc cgtacactcg
       61 gtaggggata gcaaaccgtt gaacttgcag ttttcctttg tttagcggcg ccactgctat
      121 gatcgagacc tttctgccgc cgtgatccta ggtgccatgg gatgttttgc tagattcact
      181 tcggtttggt tttattctat caatttgccg tgcgaccaac gatcggcgcg gcaattaata
      241 atcgtagcag caatttgacg gacaaaacaa ataaaatgca tctgccttga acacttcctc
      301 aatccttgca gtctggtcgt ctaaatccgc ttgccctatt ttagaatgcg caaattacgg
      361 ccgcccggcc cgctgtcgta tcctaaccgc tgacgtagcg gaccagttgc tcgatcagaa
      421 accgctggtc ggcgatgatc gacttcacga tatcgccgat cgagatgagg ccgatcaggt
      481 taccgtcctc gatgacggga agatggcgag tgcgcttttc ggttataaga gacatgcatt
      541 cttcaatcgc ttggtcgcgg tgaatatata cgaccgggct gatcatgatt tccttaacca
      601 gcgtggttgg agaagtacgg ccctgcagta cgactttgcg tgcgtattcg cgctctgata
      661 tcaggccgac gacctgcgcg ccttccgtga ccactagggc gccgacttcc ttttcggcca
      721 tgcggcggag cgcgtcgaaa accgtggcgt ccggctcgac gcaccaaaca tcggttcctt
      781 tgaccatgag gatgtctcgg attgtctgca tgggcgacct ccactcgacg cggtcggctc
      841 tgggaattta tacaaccaaa tgagcgcgca ggtccatatt cgccgcaaaa taacttgccg
      901 ccagtgcgct attcgggata aaagcaatcc ggctaaaagg aattgactct gtttggcggc
      961 ctggatatgc tccggttcgg cactctagtt cggcacgatc gaacggcgta aatttttttg
     1021 ccaagagggt gaagccaatg aagatttctg ccgagttgtt ggttttggtt ttcatcgttt
     1081 tactgtcgtc gagctgcgcg gcgcttagtg gttccggaga ttacactccc gagccaatcg
     1141 catggcacga ctataacgat tgctgattca acggattgtc cgtgcagttt ccgacggcag
     1201 cctggtcttc gttcctaaac aggtcgtcgt aatcccggcc tgaatgcatc acagcccacc
     1261 tagcatcgcg gcgccgaagg atcggtttcc ctcgtctacg gcaccagcac actctttagc
     1321 ttcgcgatca tagttgcgtc gagtttgaac agaccggcaa cgatgccttc gatggtctcg
     1381 ccgcccactg gcgtgaccgc gaggttggct ttgttcatct cggtgagaaa ttcgggatct
     1441 ttcagcgtgt cgagaaagct tttgcgcagc atgcgaacga ggtctcggtt ggtggcgggg
     1501 ggtaagaaat agagatactg caaagccgcc acgtcctgga cggcgtattt taagatttgt
     1561 ttggcttcat ccgatttggc gaagtcgatg gccaacggca cgttaggcag ctcagagtgt
     1621 cttttcggcg ccacctgcag aaccaccgag acctcgccgc tgtcaatcga cttgcgccat
     1681 agaagtttta ttccttccca tgccgcgcag aagccggcga gctcaccggc atcggctgcc
     1741 aggcgcacgt cggcggcgcc cttgtagccg tcgatgacgc ggacgggaag ggttggcagc
     1801 gctgccttca gcgtgcgcgc gatatcggat gggccggtgc cggggccgat gccgccgagc
     1861 ttcaccggtt ccttggcgac gaaccagcgc tccatgctgg tgacaccgct cgccttggtc
     1921 aacgcgcata ccggatgaaa ttccgtgggc acgcccaagt attcgaacct ccggctgtcg
     1981 aactcgatgc cgcgggcgcc caagatttgc tgcaagatga agctgccttg attgctaccg
     2041 acggtcaggc cgtcggcttt ggcgcgttga aacatgaagt tggcttggat gaagccgccg
     2101 gcaccggtca tgttctcgac gacgatggtc ggattacccg gca
//
LOCUS       DASQKS010000158         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5544522, whole genome shotgun sequence.
ACCESSION   DASQKS010000158 DASQKS010000000
VERSION     DASQKS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5544522"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..911
                     /locus_tag="VE170_04010"
     CDS             165..911
                     /locus_tag="VE170_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ14628.1"
                     /translation="MAVVSSSDFVKEIVQMVRAKHSKNHPIIGMIEDGKLDREALKGF
                     VGQFYLFFPKPFPKPIAAMLARCPDDPELEKMWIENVVEEGTGEITGTDSHKALFIRF
                     AKACGYASKEELDRVEPTPETQAFLDWRELLMSQRTWLELYACQGFCLEGTANERMTR
                     IVNGLTRHYGFNRESEDIRYWTLHMGVDEEHMKVGPLAVERYAVTEPQQVAVRAAVVK
                     TLDQFWLAFDGIVRAYVDKDPLYGRWRGGK"
     gene            927..1583
                     /locus_tag="VE170_04015"
     CDS             927..1583
                     /locus_tag="VE170_04015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYJ14629.1"
                     /translation="MEMIDLSRVIYDGMPKIPILPEVHVTKFLSLEKGHPLNVTELSM
                     PCHAGTHVDAPIHIMPNGKSIDELPVDAFVGAGAVISVKKNGGEEVTAKDLEASGVTV
                     NRGDILMLHTGWDEKFDSPDYNLHPYLSVDAAEWMVKKGIKMFGIDCITVDLPTPLRP
                     KGFDFPVHRTLLGNNVLIAENVTNLGAVVGKRTRIMALPLRVKGSDAGHARIVAEVLT
                     "
     gene            1586..2554
                     /locus_tag="VE170_04020"
     CDS             1586..2554
                     /locus_tag="VE170_04020"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ14630.1"
                     /translation="MATTKHISGIIGACLTPFDVNDRVDYKALEKEIEFLIPDCDAIT
                     VAAVEAAEYTMLSRDERKEVLKVATEMIGGRVPVILGCSSPAPREVIELAEYAATVNG
                     DFIQVLMPLRPWGGQPTIAELMEFYTQVASASPLPVTCYHNPGPGADPPQDAFVKISE
                     IDNIRYFKESSRDITKISRLIEQIELAGRGHYFTTMQPLLVTLMLGGSGATMPPPGTR
                     IGAQIVKAFRAGDLETARFWARCYALFPGKWGAYGLPPVMKSAMKHFGVDLGQPCRPY
                     APVSPRDHEQIGQFLRQVGLIGESGPSPQGLLAAGEKLRQEDTFLR"
     gene            2561..2806
                     /locus_tag="VE170_04025"
     CDS             2561..2806
                     /locus_tag="VE170_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14631.1"
                     /translation="MQLGSFGHTKPNGAAEIRTIMEWLKDLSDKLIIWFLRSSSGFGE
                     ELTADGRREQQLWREQRLRNLQGLAQRSKQRFDPQEK"
BASE COUNT          632 a          793 c          828 g          608 t
ORIGIN      
        1 tccaacccag gcgaattgcg tccacccgtc tttccaggtt ttttttcatg tggtaagtat
       61 ttctatctgt taaaccatgc agtctgtgat tgtacgcgca tgcgcatcgc gccgggttca
      121 tttaatttgt ttccgagttg ccgagaatcg aggagatagt tgccatggcg gtagtttcaa
      181 gcagcgattt cgtcaaagaa atcgtgcaaa tggtgcgggc caagcattcc aagaatcatc
      241 cgattatcgg catgatcgag gacggtaaac tcgaccgcga agcactcaaa ggcttcgtcg
      301 gccagttcta tttgtttttc cccaagccgt tcccgaaacc gatcgccgca atgttggcgc
      361 gttgcccgga cgatcccgag ctggagaaaa tgtggataga gaacgtcgtc gaagaaggca
      421 cgggagagat caccggcacg gatagccaca aagcgctgtt catccgtttc gccaaagcct
      481 gcggttacgc aagcaaggaa gagctcgacc gcgtcgaacc gacgccggag acccaggcgt
      541 ttctcgactg gcgcgagctg ttgatgtccc aacgtacatg gttggagctt tacgcttgcc
      601 agggattctg tctcgaaggc accgcaaacg agcgcatgac gcgcatcgtc aacggcctca
      661 cccgccatta tggctttaac cgcgagtcgg aagacatccg ttactggacc ctgcacatgg
      721 gcgtcgatga agaacatatg aaagtgggac cgctggcagt agaacgctac gccgtgactg
      781 aaccgcagca agtggcggtg cgcgccgcgg tggtaaaaac tttagaccag ttttggctgg
      841 cgttcgacgg gattgtccgt gcctacgtcg acaaggatcc gctctacggg cgctggcgcg
      901 gcggcaagta aacaaggagc tatgtcatgg aaatgatcga tttgtcacgg gtgatctacg
      961 acggcatgcc gaagattccg atcctgccgg aagtccatgt gacgaagttt ttgagcctgg
     1021 aaaagggcca tcccttgaac gtcactgagt tgtcgatgcc ttgtcatgcc ggcacccatg
     1081 tcgatgcgcc gatccacatc atgcccaacg gcaaatccat tgacgagttg ccggtggatg
     1141 ctttcgtcgg cgccggcgcg gtgatcagcg tcaagaagaa cggcggcgaa gaggtgacgg
     1201 caaaagattt agaagcatcc ggcgttacgg tcaatcgcgg cgatatcctt atgctccata
     1261 ccggctggga cgagaagttc gacagcccgg attataatct gcatccttat ctttccgtcg
     1321 acgccgccga gtggatggtc aaaaagggca tcaagatgtt cggcatcgac tgcatcaccg
     1381 tcgacctgcc gacgccgctg cggcccaaag ggttcgattt tcctgtgcat cgaaccttgc
     1441 tcggcaataa tgttctgatc gccgagaacg tcaccaactt gggtgcagtg gtcggcaagc
     1501 gcacgcgcat catggcactg ccgttgcggg tcaaaggcag cgacgccggc catgcgcgta
     1561 tcgttgcgga agtcttaacc tagttatggc gacgaccaaa cacatcagtg gcattatcgg
     1621 cgcctgtttg acgcccttcg acgtgaacga tcgcgtcgat tacaaggcgc tcgagaagga
     1681 aatcgaattt ctcattccag actgcgacgc gatcacggtg gcggcggtgg aagcggcgga
     1741 atacaccatg ctgtcgcgcg acgagcgcaa ggaagtgctc aaagtcgcca ccgaaatgat
     1801 cggcggtcgt gtgccggtga tcttgggctg ctccagtccg gcgccgcgcg aagttattga
     1861 actcgccgag tatgccgcca cggtgaacgg cgacttcatt caagtgctga tgccgttgcg
     1921 tccctggggc ggccagccga ccatcgccga acttatggag ttctatacgc aagtcgcttc
     1981 ggctagtcct ttgccggtta cgtgttatca caatcccggt ccgggcgccg atccaccaca
     2041 ggatgccttc gttaagatca gcgagatcga caacattcgt tatttcaaag aaagctcccg
     2101 tgacatcacc aagatctcgc gtttgatcga acagatcgag ctagccggcc gaggtcatta
     2161 ttttactacc atgcagccgc tgttggtcac gctcatgctc ggcggctccg gcgccaccat
     2221 gccgccgccg gggacgcgca tcggcgcgca gatcgtcaaa gcatttcgcg ccggtgatct
     2281 ggagacggct agattctggg cgcgctgcta tgccttgttt cccgggaaat ggggtgccta
     2341 cggtttgccc ccggtgatga agtcggcgat gaaacatttt ggtgtcgatc tcggccagcc
     2401 ttgccggcct tacgccccgg tgagcccgcg tgaccacgag cagatcggcc agtttttgcg
     2461 ccaagtcggt ctcatcggcg agtccgggcc gtcaccgcag ggtttgctcg ccgccggcga
     2521 aaagctccgg caagaagata ccttcttaag atagtcaacg atgcagctgg gcagtttcgg
     2581 acacaccaag ccgaacggcg cggcggagat tcgcacaatt atggaatggc ttaaagatct
     2641 ttccgacaaa ttgatcatct ggtttttgcg ttcgtcatcc ggtttcggcg aggaactcac
     2701 cgccgacggc aggcgcgagc aacaactctg gcgcgagcag cggctgagaa atcttcaggg
     2761 actggcgcag cgttcgaagc agcgctttga tcctcaagag aaataaattt ccgcaaaacc
     2821 aagtacccaa catgcaactc gtatcgcggc ggaaaaagat t
//
LOCUS       DASQKS010000159         6047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4286512, whole genome shotgun sequence.
ACCESSION   DASQKS010000159 DASQKS010000000
VERSION     DASQKS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286512"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VE170_04030"
     CDS             <1..686
                     /locus_tag="VE170_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14632.1"
                     /translation="AWIGSSTPATRLFYIRADHPFATIADLRRAGEPAKCGTAGRGNS
                     GYILPKLFEETLGLKLAIVTGYPGGGEVDLAIEKGEVQCNSTSLSVFFGREPFHSWRK
                     KNFVRVLIQTGNKRDPRLADVPTIYELMEQYKTPEASRRLTTVFLDAGRFGSWPIAGP
                     PGLPAERMKMLREAFVKALNDPALLAEAAKKQLDVETIRGEELESLAKEVMAQPREVV
                     ERLKDLLQN"
     gene            882..1865
                     /locus_tag="VE170_04035"
     CDS             882..1865
                     /locus_tag="VE170_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14633.1"
                     /translation="MRNTVLIIAFLVFVSFVTPAVAADKLRVAFVSPSVALSLPWVAK
                     ELGILAKHDLTAEILLITGSPRLVQSLIAGDVDVVFAGVTALTRARLRGAEVAILGAS
                     ANLSSQKLMVIRSSKVRRLEDLKGATIGVSQFGSEADTFTRNALATVGLRPERDVTIL
                     QLGGHQQVAAAMAAGKLEAGGLGGLAALTAERAGARLLSSAAELKILSPAGTFATTRG
                     YIQRKRSVVERLMRSFVEAIHYLKTNRSGSIALLQKYLGGLSAEEAAYLYDEQADLLE
                     RLPAPNEKALQAVLGRENDAKLKTMSPADFIDASFFREIEKSGLVEQLYRK"
     gene            2030..2392
                     /locus_tag="VE170_04040"
     CDS             2030..2392
                     /locus_tag="VE170_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14634.1"
                     /translation="MARIGHIALLTKDTEKLAEFYRTSFGLNEVARSGEASEHGRAIY
                     LSDGHINLAILPARNRREGIYHFGMQVEDVKGATQTALAAGATGGTANLPKDGRFAET
                     FVLDPVGTRVDLSPGWKV"
     gene            2509..2760
                     /locus_tag="VE170_04045"
     CDS             2509..2760
                     /locus_tag="VE170_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14635.1"
                     /translation="MRTNNFIKLCLLTALGLLVARAQLHAQPVLERINAIYASLAGDH
                     AALYVAQEMGLFRKYGLDVNLSYTAGAAQVIQTMMAGEL"
     gene            complement(2919..3209)
                     /locus_tag="VE170_04050"
     CDS             complement(2919..3209)
                     /locus_tag="VE170_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYJ14636.1"
                     /translation="MSIVRMIFVTVKPDQVSQAISLWKNHCAPLMIKQPGCLSEKLLE
                     CADAPGEFISYSEWDNQANIDKYRKSDAHTEIQKHSRGLQGGRATVKRYEVK"
     gene            3563..3820
                     /locus_tag="VE170_04055"
     CDS             3563..3820
                     /locus_tag="VE170_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14637.1"
                     /translation="MRSDIQNEQALYRSLVPTVIGYRLMVRYPRHLWRLLLGAMIPRD
                     CALARALTSQLDKVVTLLLLPTFFALTGMGTRIDLLYEPWQ"
     gene            complement(4065..4853)
                     /locus_tag="VE170_04060"
     CDS             complement(4065..4853)
                     /locus_tag="VE170_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14638.1"
                     /translation="MLRQLQSTSKTKNPLLKPLTRLKNEDNLQWGTRAVADMGTGGSD
                     GWTYVVLLGGNDTLSFRLRVAQSQLRSDMLPSYWSEAVLARLRGERVARARALHVPLL
                     QPAQGVFATRTNGVVDRPMRDFADPDHWPNIALIALPIAQTQILEKVETFKRSRGTLD
                     ALEHILRWLAFAWGAARTPNPLTDGIGLPSACMLEIACAAAKFDLTPGLESRASCPEA
                     IWVAAMRWHEYFENFIGQRPVGRYFTPHIYPIDEGEAANDSRRK"
     gene            complement(4873..>6047)
                     /locus_tag="VE170_04065"
     CDS             complement(4873..>6047)
                     /locus_tag="VE170_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYJ14639.1"
                     /translation="VPAKLDGTFNLLLASCYFQPEDSGGMLGNIVAQMPVRPHMTLLA
                     GDQVYLDLPLFEDLPESEPALSQFLGDKYRRNWLSETLGIAGLHALLTRAPALCIPDD
                     HEFWNNYPFKQAQLPGTWAEAGRTRWSAAAQSLYEDYQQGGPPGSAASSWRIDIDPLR
                     MLILDTRCRRGGDFAAAAGLMASEAKAALLAWETDLIAALAAGKPMIGLLAAGQALFV
                     DLPEDSAARWKDAELANYEEFDRVEGALERLATAGVPVVFLTGDVHWGRIAEAVHMPS
                     GRAMLYEVICSPSRLIDTPGTDQLALVGNSLRGIFGRAETWPRHPEPPKPPSRFGRGR
                     QFTPIKVFRRRGDQVALVQFSRAGRGLEMQVSYHAIHADPEVARPETAGPFPLLPY"
BASE COUNT         1224 a         1740 c         1833 g         1250 t
ORIGIN      
        1 tcgcctggat cggcagttcg acgccggcaa cacggctttt ctatatccgc gccgatcatc
       61 ccttcgccac catcgccgat ctgcgccggg ccggcgagcc ggcgaaatgc ggcaccgcgg
      121 ggcgcggcaa cagcggctat atcttgccca agctgttcga ggaaactctc ggcctcaagc
      181 tggcgatcgt taccggctat ccgggcggcg gcgaggtcga tctggcgatc gaaaagggcg
      241 aagtgcaatg caactcgacc tcgctgtcgg tcttttttgg ccgcgaaccg tttcacagct
      301 ggcgcaagaa aaatttcgtc cgcgtgttga ttcagaccgg caacaagcgc gacccgcgac
      361 tcgccgacgt gccgacgatt tacgaactca tggaacagta taagacgccg gaggcgagcc
      421 ggcgtttgac cacggtattt ctcgacgccg gacgctttgg ttcctggccc atcgccggac
      481 cgccggggct tccggccgaa cggatgaaaa tgctgcgcga ggcttttgtc aaagcgctga
      541 acgatccggc tctgctcgcg gaagccgcga agaaacaact ggacgtcgaa acgatacgcg
      601 gcgaggagct ggagagcctg gccaaggaag tgatggctca gccccgtgag gtggtcgagc
      661 gcttgaagga cttgctgcaa aactgacggg cgaagaattg gccgcaaaag cgcaaacggc
      721 gcaaaaagga attccaaatt gtcagggcgg gtttacaact gccatcttgt attgtccttg
      781 ctgaagattc agtaccactt gtgcaagcag ccgatctgtt tatggagggt gagtaaagcg
      841 cgaacgggta atttcttact gatgatttcg ggacctcgcc aatgagaaat acggtgttga
      901 ttattgcctt tcttgttttc gtttcatttg tcacacctgc ggttgccgcc gacaagctgc
      961 gcgtcgcctt cgtcagcccg agcgtcgccc tgtcgctgcc ttgggtggcc aaggagctgg
     1021 gaattttggc caagcatgat ttgaccgccg agattttgct gattaccggc agcccgcgcc
     1081 tggtccaatc gctgatcgcc ggcgacgtcg acgtcgtatt tgccggcgtc acggcgctta
     1141 ctcgggcccg tctgcgcggc gcggaggtgg cgatactcgg cgcttcggcc aacctttcga
     1201 gccagaagtt gatggtcatt cgcagctcga aggtgcgccg gctcgaagac ttgaaaggcg
     1261 cgaccatcgg cgtgtcgcag ttcggttccg aagccgacac ctttacgcgc aacgcgttgg
     1321 cgacggtggg actgcggccc gaacgcgatg tgacgatact gcagctcggc ggccatcagc
     1381 aggttgcggc cgccatggcg gcgggaaaat tggaagccgg cggtttgggc ggtctcgcgg
     1441 cgctcaccgc ggagcgcgca ggggcgcggc ttctcagcag tgctgcggaa ctaaagattc
     1501 tttcgcctgc cggcaccttc gccaccaccc gtggatatat tcagcgcaag cgcagcgtcg
     1561 tcgagcgctt gatgcgctcg ttcgtcgaag cgattcacta tctgaaaacc aatcgcagcg
     1621 gctccatcgc gttgctgcaa aagtatctgg gcggattgag cgccgaggag gcagcttatc
     1681 tttacgacga gcaggccgat ctgttggaac gtctgccggc gcccaacgaa aaggcgcttc
     1741 aggccgtgct cgggcgcgag aacgatgcta agttgaaaac catgtcgccg gcggatttca
     1801 tcgatgcgag cttttttaga gagattgaaa agagcggctt ggtcgagcag ttgtaccgga
     1861 aatgaaaaca agcgggtaat gcacagtttg tcgtcgctcc gggcaagccg gcgatagctg
     1921 gtgcgacccg gaatccagga gcggctggat tcccgaaaga cctcgaaatc gtcgggtgag
     1981 cattcgcgta agaacactaa ctgacttcaa accttgaagg gagaaaacca tggcacgcat
     2041 aggacatatc gcattactga cgaaggacac agaaaaactc gccgagtttt acaggacttc
     2101 tttcggtctc aacgaggtcg cgcgcagcgg cgaagccagc gaacatggca gagcgatcta
     2161 tctctcggac ggccatatca atttggcgat ccttcccgcg cgtaaccgcc gcgaggggat
     2221 ctatcatttc ggcatgcaag tcgaagacgt caagggcgcc acgcaaaccg cgctggccgc
     2281 cggcgccacc ggcggcacgg cgaatctccc caaggatggc cgtttcgccg aaaccttcgt
     2341 gctcgatccg gtgggcacgc gggtggattt gtcgccgggt tggaaagtgt gacctaactg
     2401 tacatagagt gttaggcttt tagccttcga ctgccgcagt tgattgaatg agcgatgtaa
     2461 ttcatcgttc gttagcttct cggtttgggt agaatgattg agcaggttat gcgaacgaac
     2521 aatttcatca agctctgttt gttgacagct ctcggtttat tggttgcgcg cgcacaactc
     2581 catgcacagc cggtgctcga gcgaatcaac gcgatttatg catcgctggc cggcgatcac
     2641 gccgcgcttt acgtggcgca ggagatgggc ttgttccgta agtacggtct cgatgtcaat
     2701 ctcagctaca ccgccggagc ggcgcaagtg atccagacca tgatggccgg cgaactatga
     2761 gcagcaatag ctacggaaat tcctgcgcca gtaacagcgc atgaatccca tctgaatatc
     2821 tcaacaaggc gactatcaga tcaggtcatg cgatcgcctc ttttcagagt gcgaccgcac
     2881 gacccgcctg ataaccgaac tcactcacga gctcagttct attttacttc gtagcgcttg
     2941 acggtggcgc gtccgccttg caaaccccgt gaatgtttct gaatttccgt atgggcgtcg
     3001 cttttgcgat acttgtcgat attcgcctga ttgtcccact cggaatagga gataaactca
     3061 ccgggtgcat cggcgcattc caatagtttc tcggacagac atcccggctg cttgatcatg
     3121 agcggagcgc agtggttttt ccaaagactg attgcctgcg aaacctgatc cggctttacg
     3181 gtaacgaaaa tcattctgac aatggacata ataacctcct tgttaaataa tcgaagcaaa
     3241 ctcgcatccg gtagatggtg ccgcgaaata tcaaactgct tctgtccctc ctttcgctgc
     3301 agttcgttga actacctcta gcatcagact agcaaataaa atttttcatt tcaccagcga
     3361 ttctgaagtt tcgctgggga atttttaccg gcgataattc gacgattcat aatcgttccg
     3421 caaccgcatc ggggttgaaa cgccgggcgt cgatcgatcc ggcacaacgc tggtgatgat
     3481 gacgagcgat aacttgattg cctgcagatt ttagggataa atcgctctgc gttcagtttg
     3541 cgacattcga aggtagtttt acgtgcgcag cgatattcaa aacgaacaag cactttaccg
     3601 gtcgctcgtt ccgactgtga tcggttaccg cttgatggtt cggtatccac gccatctttg
     3661 gcgcctttta ctcggggcga tgattccccg cgactgtgcc ctggcacgag ccctcaccag
     3721 ccagctcgat aaggtcgtca ccctcctgct tctcccgacg ttcttcgcat tgacgggcat
     3781 ggggacgcga atcgacttgc tgtacgaacc gtggcagtga tgaacacccg gggctgatgg
     3841 aattgatcgt tctaaacgtc gggctggact tgcaggtcat aacgcccacg ctctttgcaa
     3901 tgatggtatt gatggccctg gtaaccgcta tgatgacagc gccggtggtg cggttgctag
     3961 atccgcggcc cgccctcgaa gaggagccgg agacgataat ggaacatggg ctacgaaacc
     4021 tgcgataggt tggtgactag attcgcactt cctgtcagcg ttggctattt ccgccgggag
     4081 tcgttagccg cctcgccttc atcgatcggg tagatgtgcg gcgtaaagta gcgacccacg
     4141 ggccgctgcc cgatgaagtt ttcgaagtac tcgtgccagc gcatcgcagc gacccagatc
     4201 gcctccggac acgaggcgcg tgactcgagg ccgggcgtta gatcaaactt ggctgctgcg
     4261 caagcgatct cgagcatgca ggcgctcggc agcccaattc catcagtgag cggattggga
     4321 gttcgcgcgg ccccccaggc gaaagccagc cagcgcagta tgtgctcaag ggcgtcgagc
     4381 gtcccgcgcg agcgcttgaa ggtttcaact ttttccagga tctgtgtctg tgctatgggc
     4441 agagcgatca gcgcaatgtt gggccagtgg tccgggtcgg cgaagtcgcg catgggccgg
     4501 tcgacgacgc cgttggtgcg ggtggcaaac acgccctgcg ccggctgcag aagcggcacg
     4561 tgcagtgccc gggcgcgcgc cactctttcg ccgcgcaggc gggcgagaac ggcttccgac
     4621 cagtacgagg gcagcatgtc actacgcaac tgcgactgcg caacgcgcag gcggaaggac
     4681 agtgtatcgt ttccgccgag cagcacgacg taggtccaac cgtcggaccc gcccgtgccc
     4741 atatcggcga cagcgcgcgt tccccattgc aagttgtctt cgttcttgag ccgcgtcagc
     4801 ggcttgagca acgggttctt ggttttgctc gtagattgaa gttgacgcag cattggttgg
     4861 gtctccgtgg ggttaatagg gtaacagcgg gaacggtccg gcggtctccg gtcgcgcaac
     4921 ctccgggtcg gcgtgaatcg cgtgatagct cacctgcatt tcgagcccgc gcccggcgcg
     4981 gctgaactgc acaagggcca cctgatcgcc gcgacggcgg aacactttga tcggagtaaa
     5041 ctgccgcccg cgaccaaagc gactcggcgg tttcggcggt tcggggtgtc gcggccaagt
     5101 ctcagcgcgg ccaaaaatgc cgcgcaggct gttcccgact agcgctaatt gatccgtgcc
     5161 gggcgtgtcg atcaggcgcg atggcgaaca gatcacctcg tacagcattg cgcggccgct
     5221 gggcatgtgc acggcctcgg cgatgcggcc ccaatgcacg tcaccggtga gaaagaccac
     5281 cggcacgccc gcggtcgcca ggcgctcaag cgcgccctcc actcgatcga actcttcgta
     5341 gttggcaagc tccgcgtcct tccagcgagc cgcgctatcc tcgggcagat ccacaaacag
     5401 cgcctgccct gccgcaagca ggccgatcat aggcttgcct gccgcgagcg ccgcgatcag
     5461 atcggtttcc caagcgagga gcgccgcttt ggcttcggac gccatcagtc cagcggcggc
     5521 agcgaagtcg ccgcccctgc ggcagcgagt gtccagtatc agcatgcgca gcgggtcgat
     5581 gtcgatacgc cagctcgacg cagcgctgcc aggaggacct ccttgctggt agtcctcgta
     5641 cagcgactgg gcagccgcag accagcgggt gcgaccggcc tcggcccacg tgcccggtag
     5701 ctgtgcctgc ttgaacgggt aattgttcca gaactcgtgg tcgtcgggga tgcacagcgc
     5761 aggggctcgg gtgagcagcg catgcagccc tgcgatccca agtgtttccg acagccagtt
     5821 gcgccggtac ttgtcgccga gaaactgcga cagcgcgggt tcgctctcgg gcaggtcttc
     5881 gaagagcggc aggtccaggt aaacctgatc gccggccagc agcgtcatgt gcgggcgaac
     5941 tggcatttgc gcgacaatgt tgccgagcat gccgccgctg tcttcgggct gaaagtagca
     6001 cgacgcgagc aacagattga aagttccatc aagcttggcg ggcacag
//
LOCUS       DASQKS010000160         5237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2621518, whole genome shotgun sequence.
ACCESSION   DASQKS010000160 DASQKS010000000
VERSION     DASQKS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2621518"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1277
                     /gene="alaC"
                     /locus_tag="VE170_04070"
     CDS             99..1277
                     /gene="alaC"
                     /locus_tag="VE170_04070"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HYJ14640.1"
                     /translation="MEFQRIKRLPPYVFSIIDGMKMEARHRGEDIVDFGMGNPDLPTP
                     PHVVAKLIEAASKPANHRYSVSRGIYKLRVAIAKWYSRRYGVTIDPDSEAIVTIGAKE
                     GLSHLAWATIDPGDVVLCPSPTYPIHQYAVVLAGGDLRCIPLTTSEEFFGHLEEAIRQ
                     TWPKPKMLIISFPHNPTTQVVELDFFEKVVRAAKEHGFIVVHDLAYADLTFDGYQAPS
                     FLQAAGAKDVGVEFFSLSKSYNMPGWRVGFCVGNQEIVHALARIKSYLDYGIFQPIQI
                     AAIQALDGPQDCVEEICSMYRKRRDSLLDGLDRAGWHIPRPKGTMFVWAEIPEPAKKM
                     GSVEFCKYLLQEAQVAVSPGIGFGQYGDDHVRFALIENEHRTKQAVRKIKKALGKLLD
                     "
     gene            1411..2805
                     /locus_tag="VE170_04075"
     CDS             1411..2805
                     /locus_tag="VE170_04075"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYJ14641.1"
                     /translation="MATANSRRKVGVGLLGSGVVGEAIQDILFRDLKGQIGNDVELAI
                     RKIYTRNPRGKKWFGKRKELFTSRPEEVLDHPGIDIIIEALGSQSAAQLPQFRDYILR
                     AFRNGKSVVTSDKAVLARYGKEIWTAAKSYGRQLRFEACVGGGIPVIRSLSESFAVEQ
                     PEAVFGIVNGTCNYILSQMENSGKPYAEALREAQQKGYAETNPAADVNGSDAEAKLLL
                     LSMVAFGLQIEPGKIWRRGIEQIQAIDFRYAQRIGASTIKQLAVARRRGSAVQVFVSP
                     ALVSGQNFLASVDGATNAVCFAGKSSSGGRGARDCDYVLVGPGAGGGPTAVAVLGDVC
                     ELARGTRQFSGAPSLIPPGTLKLQREDEIDVPFYVRFVVKDRAGIVGDIGKTFGRMGV
                     NISEIWQLRHVEAELRSLMQSYRLKQKSGEILPFVMTLEPTTLRQMRRALDSIRNRDY
                     ILVDPVWFPIWSSN"
     gene            2843..4063
                     /locus_tag="VE170_04080"
     CDS             2843..4063
                     /locus_tag="VE170_04080"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HYJ14642.1"
                     /translation="MKYIILQGDGMADYPIEALGNRTPLEAARTPNMDWLAQHGVYGI
                     AHVIPKGFPPGSDVGNMSIMGYDPAVYHTGRSPLEAASMGVALGPQDIAFRCNLVTVA
                     GAGSDATMEDFTSGHISTAEAAEMIRDLGRELAGGGIEFFPGVSYRHLMVWRDGMEKM
                     ETTPPHDITDQKTAGYLPKGEGAERLLELMAASQTLLAQHPINVKRKAEGHRQATTIW
                     LWGQGRAPQLPPLTERFGITGGVISAVDIIHGLGVYAGLERIDVPGITGFLDTNYLGK
                     GEYGVRSLEKNDLVFIHVEATDEAGHMGDWKAKIQALEDFDAKVVGTVLKGMAQRRDY
                     RILLMPDHPTACALKTHVSDPVPFVLYSPQDERDNGAVGYNEKDAVKTGVVAEQAYRL
                     MESLIEGRPTWTET"
     gene            4048..4998
                     /gene="glpX"
                     /locus_tag="VE170_04085"
     CDS             4048..4998
                     /gene="glpX"
                     /locus_tag="VE170_04085"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HYJ14643.1"
                     /translation="MDRNLALEAVRVTEAAALNCARLTGRGDEKAADQAAVDAMRKAF
                     DGLGIDGTIVIGEGERDEAPMLYIGEKVGSGGPKVDIALDPLEGTTICATGGPNAIAV
                     LAMGDGGNLLHCPDTYMQKIACGPVGKGVIDIDKTPTQNLHALAEAKKCKVEDLTVII
                     LSRPRHDAIIQEVRKAGARIRLIGDGDVAAAIATTKAETGIDLLLGIGGAPEGVLAAA
                     ALRCVGGEFQGRLAPRNNEEIERAKKMGVGDIKKKFSITELAAGDVMFAATGVTDGDY
                     LRGVHFFPGGATTQSVVMRSKTKTIRVIEATHYFEHKPLY"
BASE COUNT         1144 a         1457 c         1581 g         1055 t
ORIGIN      
        1 tcacgagccc ttgcgatgcc atcgagttgc gcctctttca gtgatgcctt ataaatagga
       61 aaatactttt ccaactgcaa aagaatgagg tagcatcggt ggaatttcag cggatcaagc
      121 ggctgccgcc ctatgtcttc agcatcatcg atggcatgaa gatggaggcg cggcatcgag
      181 gtgaagatat cgtcgacttc ggcatgggca acccggatct gccgaccccg ccgcacgtcg
      241 tggccaagct catcgaagcc gcatccaagc cggccaacca ccgctattcg gtttcgcgcg
      301 gcatttataa gctccgcgta gcgatcgcca agtggtacag ccggcgctac ggcgtgacca
      361 tcgatccgga cagcgaggcg atcgttacca tcggcgctaa agaagggttg tcccatctcg
      421 cctgggcgac catcgatcct ggcgatgtcg tgctctgtcc cagtccgacc tatccgattc
      481 atcagtatgc cgtcgtgctc gccggcggcg atctgcgctg tattccgctg accaccagcg
      541 aagaattttt cggccatctc gaagaggcga tccgccagac ctggcccaag ccaaagatgc
      601 tgatcatcag ctttccgcat aatccgacga cgcaggtagt ggagctcgac ttttttgaga
      661 aagtcgtcag agcagccaaa gagcacggtt ttatcgtggt ccacgatctg gcctatgccg
      721 atcttacgtt cgatggctac caggcgccga gttttctcca agcggctggc gccaaggatg
      781 tcggcgtgga gtttttttca ttgtcgaaga gttacaacat gcccggctgg cgtgtcggtt
      841 tctgcgtcgg caaccaggag atcgtccacg cgttggcgcg tatcaagagt tatcttgact
      901 acgggatctt tcaaccgatt cagatcgcgg caattcaagc cctcgacggg ccgcaggatt
      961 gcgtcgaaga gatttgttcg atgtatcgaa aacgccgcga ctcgctgctc gacggcctcg
     1021 accgtgccgg atggcatatc ccgcggccca agggaaccat gttcgtctgg gctgaaattc
     1081 ccgagccggc aaaaaaaatg ggctctgtcg agttttgtaa atacctgctt caggaagccc
     1141 aggtggcggt gtcgccgggg atcggtttcg gccaatatgg cgacgaccat gtgcgcttcg
     1201 ccttgatcga gaatgagcac cggaccaagc aggccgtgcg caagatcaaa aaggccctcg
     1261 gcaagctgct cgattagctg ggcgaaatag cttcaaccgg gcaaaactct cgaatgaacc
     1321 gatcgtgtgc tgcgcgctcg atgggtagta cccaagtcga agctagcacg gtacccatag
     1381 tcaaatcagc agaactgaca atataggcat atggctacag cgaattcccg gcgcaaagtc
     1441 ggtgtcggac tgctcggctc cggggtggtc ggcgaggcga ttcaagatat tctgttccgc
     1501 gatctcaagg ggcaaatcgg taacgacgtc gaacttgcga tccgtaagat ttacacgcgt
     1561 aatccgcggg ggaaaaaatg gttcggcaaa cgcaaagagc tgtttaccag ccggcctgag
     1621 gaagtgctcg atcatccggg gatcgatatc atcatcgaag ctctgggctc ccaatcggcg
     1681 gcgcagttgc cgcagtttcg cgattatatt ttgcgcgctt tccgtaacgg taagtcggtg
     1741 gtaacgtccg acaaagccgt cctggcccgc tatggaaaag agatttggac ggcggcaaaa
     1801 agttacggac gccagttgcg cttcgaagcg tgcgtcggcg gcggcattcc ggtaatccgt
     1861 tcgctcagcg agagtttcgc cgtcgagcaa cccgaagcgg tgttcggcat cgtcaatggc
     1921 acctgcaatt atattctctc gcagatggag aatagtggga aaccctacgc cgaggcattg
     1981 cgcgaggcgc agcaaaaggg ctacgccgaa accaatccgg cagccgacgt caatggcagc
     2041 gacgccgaag ccaaactgct cctgctcagc atggtcgcct tcggcttgca gatcgagccc
     2101 ggcaagattt ggcgccgagg gattgaacaa atccaagcca tcgatttccg ctatgcccaa
     2161 cgcataggcg ccagcaccat caaacagctg gcggtggcgc gccggcgcgg ttcggctgtg
     2221 caagtgtttg tttcgccggc actagtctca ggacaaaact ttctcgccag cgtcgacggt
     2281 gcgaccaacg cggtgtgctt tgccggcaag agcagcagcg gcggacgcgg cgcgcgggac
     2341 tgcgactatg tgttggttgg cccgggcgcc ggcggtggac cgacggcagt ggcggtgctc
     2401 ggcgatgtct gcgagctggc caggggcacg cgtcaattca gcggcgcgcc gagtttgatt
     2461 ccaccgggca ccctgaagct gcaacgggaa gatgaaatcg acgtgccgtt ctatgttcgc
     2521 ttcgtggtca aggaccgcgc cggcatcgtc ggcgatatcg gcaagacctt cggccgcatg
     2581 ggcgtaaata tttccgagat ctggcagctg cgccatgtcg aagcggaact gcgcagcctg
     2641 atgcaaagct atcggctcaa acaaaagtcg ggagaaattt tgcccttcgt gatgaccctc
     2701 gaacctacaa ccttgcggca gatgcgccgg gcgctggact ctattcgcaa tcgcgactat
     2761 atcctagtcg atccggtatg gtttccgatc tggagttcga actagatact ggcaagcaga
     2821 aaagttaagg cggttttgcg cgatgaaata tatcattctc caaggcgacg gcatggccga
     2881 ctacccgatc gaagcgctgg gcaacaggac gcccttggaa gcggcgcgca cgcccaatat
     2941 ggactggttg gcgcagcatg gggtctatgg cattgcccat gtgattccta aaggctttcc
     3001 ccccggcagc gatgttggca acatgagcat catgggttat gacccagcgg tgtatcacac
     3061 cggccgctcg cccttggagg ccgccagcat gggtgtggcg ctggggcctc aagacatcgc
     3121 ctttcgttgc aatctggtca ccgtggcggg cgctggctcc gacgcgacca tggaagattt
     3181 tacctcgggc catatctcca ctgctgaagc cgcggagatg atccgcgacc tgggccgcga
     3241 gctcgctggc ggcggcatcg agttttttcc cggtgtgagt taccgccatc tgatggtttg
     3301 gcgcgacggc atggaaaaga tggaaaccac gccgccccac gacatcaccg accaaaagac
     3361 cgccggttat ctgcccaaag gcgagggcgc tgagcgcctg ctcgagctga tggcggcgtc
     3421 gcagactttg ttggcgcagc atccgataaa tgtaaagcgc aaggctgagg gacatcgcca
     3481 agcaacgacg atttggctgt ggggtcaggg cagggcgccg cagttgccgc cactgaccga
     3541 gcgcttcggc attaccggcg gcgtgatttc cgccgtcgat atcattcatg gtttgggcgt
     3601 atacgccggg ctggagcgca tcgatgtgcc ggggatcacc ggttttctcg atactaatta
     3661 tctcggcaag ggcgaatatg gcgtgcggtc gttggagaag aacgatttgg tgtttatcca
     3721 tgtcgaggcc accgacgagg ccggccatat gggcgactgg aaggcgaaga tccaggcgct
     3781 ggaggatttt gacgcaaagg tcgtcggcac tgtgttgaaa ggcatggcgc agcgccggga
     3841 ttatcgaatt ttactcatgc ccgaccatcc gaccgcctgc gcgctcaaga cccatgtttc
     3901 cgatccggtg cccttcgtgt tgtattcgcc tcaagacgag cgtgacaacg gcgccgttgg
     3961 ctataatgaa aaagacgcgg ttaaaaccgg cgtcgtggcg gaacaagcat accggctcat
     4021 ggagtcgctg attgaaggga gaccgacatg gacagaaact tagctcttga agcagtgcgt
     4081 gtgaccgaag cggcggcgct caattgtgcc cgcctgaccg ggcgcggcga tgagaaagcc
     4141 gccgatcaag cggcggtgga tgccatgcgc aaggccttcg atggtttggg tatagatggc
     4201 acgatcgtga tcggcgaggg cgaacgggac gaagcgccga tgctctatat cggcgaaaag
     4261 gtcggctccg gcggccccaa agtggatatc gccctcgatc ccctggaagg cacaacgatt
     4321 tgcgccaccg gcggccccaa tgcgatcgcg gtgctcgcca tgggcgatgg cggtaattta
     4381 cttcactgtc ccgacaccta catgcagaag atcgcctgcg gtccggtcgg caaaggcgtc
     4441 atcgatatcg acaaaacccc gacgcagaat ctccacgccc tggcggaagc gaagaagtgc
     4501 aaggtcgaag atctaaccgt tatcattcta tcgcggccgc gccatgacgc aatcattcaa
     4561 gaagtgcgca aggccggcgc gcgcattcgc ttgatcggcg acggcgacgt cgccgcggcg
     4621 atcgccacca ccaaggcgga gaccggcatc gatttgcttc ttggtatcgg cggcgcgccg
     4681 gaaggggtct tggccgccgc agcgttacga tgtgtcggcg gcgagttcca agggcgcctg
     4741 gcgccacgca acaacgaaga aattgaacgc gcaaaaaaaa tgggcgttgg cgatatcaag
     4801 aaaaaattct ccataaccga actggccgcc ggcgatgtca tgttcgccgc caccggagtg
     4861 accgacggcg attatctgcg cggcgtgcac tttttccccg gcggcgcgac cacacaatcg
     4921 gtggtcatgc gttcaaagac caaaacaatt agggtcatcg aggcgactca ttatttcgag
     4981 cacaagccgc tctattagta aacgttcccg gagatgagtg aatgcagtaa gaccgctagt
     5041 tgaagcgcgg tgtgctttga tccactagcg gtcgcaatca tggctcagaa aaaaaaagta
     5101 aaaaaagccg gctggctcaa aatagttctt atctatactt tctttccact cgtcatctgg
     5161 tcgctggcat tcttcgcgtg gttgtattgg gagagcatca ttgcgccatt ctcaacggat
     5221 aaagcggaag tgcgcaa
//
LOCUS       DASQKS010000161         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3026658, whole genome shotgun sequence.
ACCESSION   DASQKS010000161 DASQKS010000000
VERSION     DASQKS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026658"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VE170_04090"
     CDS             <1..1187
                     /locus_tag="VE170_04090"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYJ14644.1"
                     /translation="LWISLGGVLGGLFNALVAPQVFSSVLEYPLMMIAAAFARPVVSG
                     TDNIAAGKKMDWLLPVLFCVATLLLLELGKSVSALSRANLHLMIFGVSGIFCLFFADR
                     PVRFGLGLIALFLIAARFPQPYGQELFHDRSFFGAYRVTHDLSSKRRLLFHGTTIHGA
                     QSSEPAKHLIPLSYYHHSGPAGQVLLAGARGGATGKVAIVGLGTGALACHGGAAQQFT
                     FYEIDPMVEMIARDPRLFTYLRDCSPKIDVVIGDARLTLAHVPDHSHDIFVLDAFSSD
                     VIPTHLLTRQAIELYLRKTAADGILLIHISNRYMDLAPVLDRLAQELRLTALIQDDFE
                     LRPEDMAEGKTPSRWILLSRDSRGVQNFAASPRWQRLDGRLGGDLWTDEFSDVLKVLR
                     WR"
     gene            complement(1194..>2200)
                     /locus_tag="VE170_04095"
     CDS             complement(1194..>2200)
                     /locus_tag="VE170_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14645.1"
                     /translation="ALFAIVQPQRLNAASGDDFYRGKTIRVIVGFSAGGGFDQYARTI
                     ARHMPKHIAGNPAMIVDNMGGAGSRLAANYLYKASAPDGLTIGNFIGSLLLQQILGDQ
                     GVEFDGKRFEWLGAPVQDETVCALTKASGVTSLDQWFAAKKPVKLGGEAPGANDSDVP
                     RMLQATLKLPLQLIEGYKGTSFIRIAAEAGEVDGGCWTWASIRSTWKKGLDSGQVIPI
                     IQVNAKKAHDIPNVPNAYDHAKTAEGKALLEAGVHAPSAILRAYALPPGTPKDRLTIL
                     RDAFNATMKDKEFNAEIVKGNLELDPLTGAQVESLARKLYQVDQSLVARLREILVPRK
                     "
BASE COUNT          384 a          635 c          661 g          520 t
ORIGIN      
        1 atctctggat atcgttaggg ggtgtgcttg gtgggttgtt caatgccttg gtggcgccgc
       61 aagttttcag cagtgtcctt gaataccctc tgatgatgat tgcggctgct tttgcccgtc
      121 cagtcgttag cggtactgac aatatcgcgg cgggcaaaaa aatggattgg ctccttccgg
      181 tgcttttttg cgtggccacg ctgctgctac tcgaactagg caaatccgtg tcagctttgt
      241 cgcgtgccaa tcttcacttg atgattttcg gcgtcagcgg catcttttgc ttgtttttcg
      301 ccgatcggcc agtgcggttc ggactgggtt tgatcgccct cttcttgatc gctgcgaggt
      361 ttccacaacc ctacggtcag gagctattcc atgaccgtag ttttttcggc gcctaccggg
      421 tgactcacga cctctccagt aaacgccgac tgctgtttca tggcacgacg attcacggcg
      481 cccagagctc cgagcctgcg aagcatttga ttcccttgtc gtactatcat cacagcggtc
      541 ccgcagggca agtgctgtta gccggcgccc gcggcggcgc cacgggcaag gtcgccatcg
      601 ttggccttgg caccggcgcg ctagcctgtc acggcggcgc cgcgcaacaa ttcacgttct
      661 acgagattga tccgatggtg gaaatgatcg cgcgcgaccc gcgtttgttc acctatttgc
      721 gcgactgttc gccgaagatc gatgtcgtca tcggcgacgc gcgcctaacg ctagcccatg
      781 tgccggacca ctcgcacgat atttttgttc tggatgcttt tagctcggac gtgattccca
      841 cccatctatt gacacgccag gcgattgagc tctatctgcg taaaaccgct gccgatggca
      901 tactgctgat ccatatttca aaccgttata tggatctagc gccggtgctc gaccggctcg
      961 cccaggaatt gagattgacg gcgttgattc aagacgactt cgagctcaga cctgaagata
     1021 tggccgaggg taaaactccg tcgcgctgga ttctattgtc gcgtgacagt cgaggggtgc
     1081 aaaacttcgc tgccagtccg cgctggcagc ggctcgacgg acggctcggc ggcgatctct
     1141 ggaccgacga attctccgat gttctcaagg tgctgcgctg gcgctagcgc cggctatttc
     1201 ctcggcacca agatctcgcg cagcctggca accaagcttt gatccacttg ataaagtttg
     1261 cgggcgaggg attcgacctg ggcgccggtg agcgggtcca gctcgaggtt gcctttaacg
     1321 atctcggcgt tgaattcttt gtctttcatg gtcgcattga acgcgtcgcg caaaatcgtc
     1381 aatcggtctt tgggtgttcc cggcggcagc gcgtaggcgc gcaggatcgc cgacggcgcg
     1441 tgtacgccgg cttccagcag cgctttgcct tcggcggtct tggcgtgatc gtaggcgttg
     1501 gggacattgg ggatgtcgtg agccttcttg gcgttgacct gaatgatcgg aatgacttgc
     1561 cccgagtcga ggcccttttt ccaagtcgag cggatcgacg cccaagtcca gcagccgccg
     1621 tcgacttcgc cggcctcggc ggcgatgcga atgaacgaag tgcctttgta gccttcgatc
     1681 aattgcagcg gcagcttcaa ggtcgcttgc agcatgcgcg gcacgtcgga gtcgttggcg
     1741 ccgggcgctt cgccgccgag cttcaccggc ttcttggccg cgaaccattg gtcgaggctg
     1801 gtgacgccgc tcgccttggt cagcgcgcac accgtttcgt cctgcaccgg cgcacccaac
     1861 cactcgaagc gcttgccgtc aaattccaca ccctggtcgc cgaggatttg ttgcaaaagc
     1921 agcgagccga tgaagttgcc tatggtcaaa ccatcgggtg cgctggcttt atagagataa
     1981 ttggcggcaa gccggctgcc ggcgccgccc atgttgtcga cgatcatcgc cggattgccg
     2041 gcgatatgct tcggcatatg acgcgcgatg gtgcgagcat attgatcgaa gccgccgccg
     2101 gcggagaaac caacgatcac ccggatggtc ttgccgcggt agaagtcgtc gccagaggcg
     2161 gcattgagtc gttgcggttg aacgattgcg aagagtgcga
//
LOCUS       DASQKS010000162         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2623837, whole genome shotgun sequence.
ACCESSION   DASQKS010000162 DASQKS010000000
VERSION     DASQKS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2623837"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VE170_04100"
     CDS             complement(<1..818)
                     /locus_tag="VE170_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ14646.1"
                     /translation="MEEKISFPSDGLKIAGLLDTPADYTTGERRPAFITLHGFGGNKD
                     GQGQKVVAQQMSQWGYVALRLDFRGCGDSEGEKGRIICLDQVADTRNAITYLATRPEV
                     DPARIALIGSSFGAAVAVFTGGADSRVAAVISCGGWGDGERKFRGQHPGPEKWAKFTA
                     MLEEGKRHREQTGKSLMVPRYDIVPIPEKLRNRMSHGSIMEFPAETAQSMNDFRADDQ
                     VGNISPRPLLLIHSANDSVTPTYESIEMFKKARQPAELHLLSDVDHFTFNEENPR"
     gene            complement(864..1898)
                     /locus_tag="VE170_04105"
     CDS             complement(864..1898)
                     /locus_tag="VE170_04105"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14647.1"
                     /translation="MGALIKNSMRWLAIAALLCTAVAVSANPYLPKSGEALIPIRVGT
                     CGTSGGFIHFYAALAGGLFEKYGIKVEHVTLRGGAVTITALASDGIQFLYCSADPQIP
                     RMAAGAEAKIVSSLIVGLPWVLMGKTDIKRPADLRGRTIPVSRPGGLTDHLAKAVLKK
                     NNLTTQDVKLLHIGGTGQLEPYNALLQGLADAVLLTPPLDVRAKRDGFHLIFSLQDLD
                     VPSIYSSILTNLKTLKERPQLVQRFVAAMSETIYFVEKNPERASQSLGKALNINDPEI
                     LQSAYQAYARKLVNRRMTVPGKTVAGAVELAREEVAQIRRKPEELYDNSFTDNLVTSG
                     FLKELWGSLP"
     gene            complement(1995..2450)
                     /locus_tag="VE170_04110"
     CDS             complement(1995..2450)
                     /locus_tag="VE170_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14648.1"
                     /translation="MPLHQPVYFEDVHIGETIPERQHGPLTIVDTVRWAGLQENSQHL
                     HFDRDHVRQHNGLKTFIASGAYRQALLMRLLTDWIGPRGWLRKITVRQTYSTFEGDLM
                     RFGAKIVDKSADPADPWIDCELAAKNQDDKQILTGHCRLILPCRARSRN"
     gene            complement(2456..>3088)
                     /locus_tag="VE170_04115"
     CDS             complement(2456..>3088)
                     /locus_tag="VE170_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ14649.1"
                     /translation="AKKNGEGVIVTVSLREALESFAEQAMVEYTFSGNITERRGSKGA
                     ITAISGALPCRDGHWVISQIHRPGRWNKFVEWVNDPELAADPSLAEEANQIKRRDFIL
                     DRVLSWASQHAKNELVEEAQKRHFPASPVSTPLDLIDDPQLNARGFLKRIDHRQFGSI
                     PFPQGAIATMRKSELTAAPRLGEHTGEILSRLGYSAGDQQSFAAAGAVQG"
BASE COUNT          563 a          881 c          974 g          670 t
ORIGIN      
        1 cgcggattct cttcgttgaa ggtaaaatgg tcgacgtccg acagcaggtg cagctccgcc
       61 ggctgccggg ctttcttgaa catctcgatg gactcgtagg tcggcgtcac cgagtcgttg
      121 gcgctatgga tgagcaagag cggccgcggt gaaatgttgc cgacctgatc gtcggcgcgg
      181 aagtcgttca tgctctgggc ggtctcggcg gggaactcca tgatcgagcc atggctcatg
      241 cggttacgca gcttttccgg gatcggcacg atgtcgtagc gcggcaccat gagggatttg
      301 ccggtctgct cacggtggcg ttttccctct tcgagcatgg cggtgaactt cgcccacttc
      361 tccgggccgg gatgctggcc gcggaatttc ctttcgccgt cgccccagcc gccgcaggag
      421 atcaccgcgg cgacacgaga gtcggcgccg ccggtgaaga ccgccaccgc cgcgccgaaa
      481 ctgctgccga tgagggcgat gcgcgcgggg tcgacttccg gccgggtcgc cagatacgtg
      541 atagcgttgc gggtgtcggc gacttgatcg aggcagatga tccggccctt ttcgccttcg
      601 ctgtcgccgc agccgcggaa gtcgagccgc agcgcgacgt aaccccactg gctcatttgc
      661 tgggcgacga ccttttggcc ttggccatcc ttgttgccgc caaagccatg cagcgtgatg
      721 aatgccggcc ggcgctcgcc ggttgtgtag tcggcaggag tgtcgaggag accggcgatt
      781 ttcaaaccat ccgacggaaa actgattttc tcttccacgt tctgctcctt atttatatgc
      841 gcctgtgcga ttgcgtgcgc agtctacggc agactgcccc acaattcttt caggaaaccg
      901 ctcgtcacca ggttgtcggt gaaactgttg tcgtaaagct cttcgggttt gcggcggatt
      961 tgcgccactt cttcgcgcgc cagctcgacg gctccggcga cggttttgcc gggaaccgtc
     1021 atgcgccggt tgaccagttt gcgcgcgtaa gcttgatagg cggattgcaa tatttccggg
     1081 tcgttgatgt tgagcgcttt gcccaggctt tggctggcgc gctcgggatt cttctcgacg
     1141 aaataaattg tttcgctcat cgccgcgaca aagcgttgca cgagctgcgg ccgctcttta
     1201 agagttttca gattggtcag gatcgaactg tagatcgacg gcacgtccaa gtcctgcaag
     1261 ctaaagatca gatgaaaacc gtcgcgcttg gcgcgaacat cgaggggcgg cgttaatagg
     1321 acggcgtcgg ccaagccctg caagagggcg ttatacggtt ccaactggcc ggtgccgcca
     1381 atgtgtagga gcttaacatc ttgagtggtt aaattatttt tcttgagcac ggctttggcg
     1441 aggtgatcgg tcaatccccc cggccgcgaa accgggatgg tccggcccct gagatcggcc
     1501 ggccgtttga tgtcggtttt tcccattaac acccagggca aaccgacgat cagcgagctg
     1561 acgatcttgg cttcggcgcc agcggccatg cgcggaatct gcgggtcggc cgagcagtac
     1621 aaaaattgga taccgtccga tgccagcgca gtgatggtga cagcgccgcc gcgcaaggtc
     1681 acatgctcga ccttgatgcc gtacttttcg aacaggccgc cggccagcgc cgcgtagaaa
     1741 tgaatgaagc cgccggaggt gccgcaggtg ccgacgcgga tggggataag cgcttcacct
     1801 gatttcggca ggtagggatt ggcgctcacc gcaaccgccg tgcacaacag agcggcgatg
     1861 gcgagccagc gcatcgaatt tttgatgagc gcgcccatgc aagcctggtc tataacaaag
     1921 ccgggggttg gagaaaaggg ccaggctatc gtgagtcgat ggcgacggcg tcggcgccga
     1981 ccactcggtt cgtgctaatt tcgagagcga gcacggcacg ggagaattaa ccggcaatgg
     2041 ccggtgagga tttgcttatc gtcctgattt tttgctgcga gctcacagtc gatccatgga
     2101 tcggctgggt cggcggattt gtcgacgatc ttggcaccga agcgcatcag atcgccttcg
     2161 aaagttgaat aggtctgacg caccgttatt ttcctgagcc atcctcgcgg gccgatccaa
     2221 tcggtcaaca gtcgcatgag caacgcctgg cggtaggcgc cgctggcgat aaaagtcttc
     2281 agcccattgt gttggcgcac gtgatcgcgg tcgaaatgca ggtgttgcga gttttcctgc
     2341 aggccggccc aacgcaccgt gtcgacaatg gtcagcggac catgctggcg ctccgggatc
     2401 gtctcgccga tatgcacgtc ttcgaaatag accggttgat gcagcggcat gggttttatc
     2461 cttgaaccgc tccagcggcg gcaaaggatt gctgatcgcc ggctgagtag cccaaccgcg
     2521 aaagaatctc tccggtgtgt tcgcccagcc gcggcgccgc tgtgagttcg ctcttgcgca
     2581 tggtggcgat ggcgccctgg ggaaatggga ttgagccgaa ttgccgatga tcgatgcgct
     2641 tgagaaatcc gcgcgcgtta agttgcggat cgtcaatcaa atccaacggt gtcgagaccg
     2701 gcgaagccgg aaagtggcgt ttctgggctt cctcgaccag ctcgttcttg gcatgctggc
     2761 tggcccacga aagcacccgg tcgagaatga agtcacgccg tttgatttgg ttcgcttctt
     2821 cggcgaggga tggatcggcc gccagctcgg gatcgttcac ccattcgacg aacttattcc
     2881 accgccccgg tcgatgaatc tggctgatca cccaatggcc gtcgcgacag ggcagcgcgc
     2941 cggatatagc ggtgatggcg cccttgctgc cgcgccgttc ggtgatattg cctgagaatg
     3001 tgtactcgac catcgcttgt tcggcgaagc tctcgagcgc ttcgcgcagc gacacggtaa
     3061 cgatcacgcc ttcgccgttt ttcttggc
//
LOCUS       DASQKS010000163         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_946755, whole genome shotgun sequence.
ACCESSION   DASQKS010000163 DASQKS010000000
VERSION     DASQKS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946755"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VE170_04120"
     CDS             complement(<1..908)
                     /locus_tag="VE170_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14650.1"
                     /translation="MTPPTQISEVVHTKRASRLILLSVCLGQALVGLDQRALTVALPT
                     LTTTFNTAFTTIQWTILVYDLVLIGLIITMGRLGDMFGRRRFYSLGFLIFVTASAICG
                     MTQTSGQLIFFRAVQAIGGSMIAANGRAIVSVNLPAEERGRALGLTSTAFHIGFLIGP
                     ALGGFLIDGLGWRWIFYINMPFSLAGAYLAWKVVPETRMHGKTSVDFAGAILLLLTNG
                     LFIYAVDQFPRVGWRHPAFFLTLAMAAVALVFLLRAESRAKLPILVLSMFRARLFSAG
                     IASLFVLAATLSAINFLLPFYLQSLLG"
     gene            complement(921..2357)
                     /locus_tag="VE170_04125"
     CDS             complement(921..2357)
                     /locus_tag="VE170_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14651.1"
                     /translation="MADSTISDAQRRHSQLVLYQVSLGLAVVAINHRAIQVALPTLTH
                     VFHSDLSFIQWVLLVYDLCIIGLVLTLGRLGDLFGRKWIYVSGFLIFVMGSALCGLAQ
                     SSTQLILFRVFQGIGGAMIMANGRALVTINSPASERGKALGFTSMAFHVGYITGPTLG
                     GFIIDAVGWRWVFFLTVPIGLICAYFGWKILKERPVSEGQVQLDFAGAAYLLLTNICF
                     LYALNRLPHQGFQDTLVVVFFFVAAVTLFLFIRTEQKATTPILSLSLFRNRLFTAANL
                     SLFFLTSTQAAVSVLIPFYLQNLMGFTPTQMGWILIGGSVVIIVLAPVAGRLSDRFGS
                     RLLCTVGAAIMMVGQYLIGSLTLQSTVFQMVVPQVLIGLGWALFNSPNQSAIMGTVPR
                     DKVGAASGMTLTTARIGGAMGVAISGAIMTYALGANGLTTEQIESPASWTAEPQVFLK
                     SFSFTTHLINAFAIFAVIFAAMRGPRKD"
     gene            complement(2517..2681)
                     /locus_tag="VE170_04130"
     CDS             complement(2517..2681)
                     /locus_tag="VE170_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14652.1"
                     /translation="MSPSAKKIPIGLAKVRKQLPPPGKIMQSRRDLHRKRAKAELRRE
                     LKGQLSGGHS"
     gene            complement(2678..>4171)
                     /locus_tag="VE170_04135"
     CDS             complement(2678..>4171)
                     /locus_tag="VE170_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ14653.1"
                     /translation="YISDRFLPDKAIDLIDEAASRLRIEIDSMPIEIDEVERKIMQLE
                     IERQALKREEDKASMNRLAQLERDIQNLKETSSGLKTHWQNEKAAIQQLRGIKEKIEA
                     TKVEEQKAQREGNLNKAAELHYGTLTQLQKDSDAANRKLAELQTHQKMLKEEVDAEDI
                     AEVVAKWTGVPVSKMLEGEVQKLLKMEERLKLRVVGQENAIIAVSNAVRRARAGLQDS
                     NRPIGSFIFLGPTGVGKTELCRALAEFLFDDEQAIVRLDMSEYMEKHSVSRLIGAPPG
                     YVGYDEGGQLTEAVRRRPYSVVLFDEIEKAHPDVFNALLQLLEDGRMTDGQGRTVDFK
                     NTVIIMTSNLGSQYIQELAGKDRKQMEDYVGNALREAFKPEFLNRVDETIIFSSLGRD
                     EIKSIVDIQLKRLRHNLANRKMALEITEPAKALLADKGYDPVYGARPLKRTIQRLIQD
                     PLAVKILAGEFKEGDTVKVEVDDDDLSFTHGPAVVASDPTFEQRILH"
BASE COUNT          932 a         1143 c         1228 g          868 t
ORIGIN      
        1 ccgagcaagc tctgcaaata aaatggcaaa agaaaattaa tcgccgacag cgtggcggcg
       61 agcacgaaca agctggctat gccggcgctg aacagccggg cgcgaaacat cgacaaaacc
      121 agaatcggca gtttggcgcg gctttcggct cgtaggagga agaccagcgc aacggcggcc
      181 atagccaagg tcagaaaaaa cgccggatga cgccagccga cccgcggaaa ctggtcgacg
      241 gcgtagatga acaagccatt ggtcaatagc agcagaatgg cgccggcgaa atccaccgag
      301 gttttgccgt gcatgcgtgt ttcgggaacg actttccaag ctaaataagc gccggccagg
      361 ctaaagggca tgttgatata aaaaatccag cgccagccga ggccgtcgat caaaaatcca
      421 cccagtgcgg ggccgatgag aaagccgatg tgaaacgccg tcgacgtcag ccccaaggcg
      481 cggccccgct cttccgccgg caagtttacg gacacgatgg cgcggccgtt ggcggcgatc
      541 atcgagccgc cgatggcttg aaccgcgcga aaaaaaatta gctggccgct ggtttgagtc
      601 atgccgcaga tcgccgatgc ggtgacgaag atcaaaaacc ccaggctgta gaaacggcgc
      661 cggccgaaca tatcgccaag cctgcccatg gtgatgatca ggccgatcaa gactagatcg
      721 tagacgagta tggtccactg gatggtggtg aaagcggtat tgaacgtcgt cgtcagcgtc
      781 ggcaaggcga cggtgagggc gcgctggtcg aggccgacta aagcctggcc gaggcagacg
      841 ctcaataaaa tcaagcgcga ggcgcgcttg gtgtggacaa cctcgctgat ttgcgttggc
      901 ggcgtcattc ttgccgtcgc ctaatccttg cgcgggccgc gcatggccgc gaagatcacc
      961 gcaaaaatcg caaaggcgtt gatcaagtgg gtggtgaagc taaaggattt caaaaatacc
     1021 tgcggctctg ccgtccagct cgccggcgac tcgatctgct ccgtggtcag gccattggcg
     1081 cccaaggcgt aggtcatgat cgcgccggat attgctacgc ccatggcgcc gccaattctc
     1141 gccgtagtga gcgtcatgcc ggaggcggcg cccactttat cgcgcggtac ggtgcccatg
     1201 atcgcgctct gattgggtga gttaaacagc gcccaaccca agccaattaa aacttgtgga
     1261 acgaccattt ggaaaactgt cgattgcaaa gtcagcgagc cgatcaaata ttgaccgacc
     1321 atcatgatcg cggcgccgac cgtgcaaagc agccgcgaac caaagcggtc cgaaagacgg
     1381 ccagccaccg gggctaacac gatgatcacg accgagccgc cgatcaagat ccagcccatc
     1441 tgggtcggtg tgaagcccat caggttttgc aaataaaacg ggatcaagac gctcactgcg
     1501 gcctgcgttg aagtcaaaaa gaataggctc agattggccg cggtgaacag ccggttacga
     1561 aacaaggata aactcagaat cggcgtggtc gctttttgct cggtgcgaat aaacagaaac
     1621 agcgtcacgg cggcgacgaa gaaaaagacc acgaccaggg tatcttggaa gccctgatga
     1681 ggtagccggt tgagggcgta gaggaaacag atgttggtca ggagcagata tgcggcaccg
     1741 gcaaaatcca gctggacctg tccttcgctg acggggcgct ctttgagaat tttccagccg
     1801 aaataggcgc agatcagacc gatgggaacg gtgaggaaga aaacccaacg ccagcccacc
     1861 gcgtcgatga tgaaaccgcc caaggtcgga ccggtgatat agccgacatg aaacgccatg
     1921 gacgtgaatc cgagcgcttt gccgcgttca gacgcgggcg agttgatcgt aaccagggcg
     1981 cggccattgg ccatgatcat ggcgccgccg atgccttgga atacgcgaaa caagattaac
     2041 tgggtcgacg attgggccag gccgcaaagc gccgaaccca tgacgaagat cagaaagccg
     2101 gaaacataaa tccacttacg tccgaacagg tcgccgagcc tgcccagggt caggaccaag
     2161 ccgataatgc agagatcgta aaccagcaat acccactgaa tgaacgacaa atcgctgtga
     2221 aaaacatggg tgagggtggg caaagcgact tgaattgcgc gatggttgat cgccaccacg
     2281 gcgaggccga gggagacttg ataaagcacc agctgggagt ggcgtcgctg cgcgtcgctg
     2341 atagtcgaat cggccatgaa ttaaaattta tatttcttaa cggtataccg tttgtggaag
     2401 ggtgtcgcca caaaggttca gagtaggggc gaaagatttt tcgcccctac taaaatgagg
     2461 gggcaggccg atatcgcccg ccccctcttc tgattgtcgc gcttatcaaa cgaagattac
     2521 gaatggccgc cgcttaattg acctttgagc tcgcggcgta gttcggcttt ggcgcgcttg
     2581 cgatgcaggt ctctcctgct ctgcatgatt tttcccggcg gcggcagctg cttgcgtact
     2641 ttcgctaggc cgatggggat cttttttgcc gatggactca atggagtatc ctctgctcga
     2701 aggtcggatc gctcgctact accgccggac catgggtgaa gctgaggtca tcgtcgtcaa
     2761 cttccacctt cacggtgtcg ccctctttga attcgccagc aagaatctta accgcgagcg
     2821 ggtcctgaat caatcgctga atggtgcgtt tgagcgggcg ggcgccgtag accggatcgt
     2881 accccttgtc ggcgagcaag gctttggccg gctcggtgat ctcgagcgcc atcttgcggt
     2941 tcgccaaatt gtgccgcagg cgcttcagtt gaatatcgac gattgacttg atctcgtcgc
     3001 ggccgaggct gctgaaaatg atcgtctcgt cgactcggtt caaaaactcc ggcttgaagg
     3061 cttcgcgcaa ggcgttgccg acatagtctt ccatctgctt acggtccttg ccggcgagct
     3121 cttggatata ttggctgccg aggttagagg tcatgatgat gacggtgttt ttgaaatcga
     3181 cggtgcgccc ctgtccgtcg gtcatgcggc catcttcgag cagctgcaac agcgcgttga
     3241 acacgtcggg gtgagccttc tcgatttcat cgaacaacac cactgaatag ggccggcggc
     3301 gcaccgcttc ggtcagttgc ccgccttcat cgtagccgac gtagcccggc ggggcgccga
     3361 tcaaccgcga tacggaatgt ttttccatgt actccgacat gtcgaggcga acgatcgctt
     3421 gctcgtcatc gaagagaaac tcggccagcg cccggcagag ttcggtcttg ccgacgccgg
     3481 tggggccgag aaaaataaac gagccgatcg gccgattgct atcttgcaat ccggcgcggg
     3541 cgcggcgcac ggcgttggat acggcgataa tggcgttttc ctggccgacg acccggagct
     3601 ttagccgctc ttccattttg agtaactttt gcacttcccc ttcgagcatc ttcgacaccg
     3661 gaacaccggt ccatttggcg accacttcgg cgatgtcttc ggcgtcgact tcttccttga
     3721 gcatcttttg atgggtttgc agttcagcga gtttccgatt ggctgcatcg ctgtctttct
     3781 gcaactgcgt caaggtgccg tagtgcagct cagccgcctt gttgagattg ccctcgcgct
     3841 gggctttttg ttcttcgact ttagtcgctt cgatcttttc tttgattccg cgcagttgtt
     3901 ggatggcggc tttttcattc tgccaatggg ttttgagacc gctcgaggtt tccttcaaat
     3961 tttgaatatc ccgttcgagc tgcgccaaac ggttcattga cgctttgtcc tcttcgcgct
     4021 tgagtgcctg acgctcgatt tccaattgca tgatcttgcg ctcgacctcg tcgatctcga
     4081 tcggcatgct gtcgatctca atgcgcaggc gcgatgcggc ctcgtcgatc aagtcgatgg
     4141 ccttgtccgg caggaaacga tcgctgatgt a
//
LOCUS       DASQKS010000164         9864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5547848, whole genome shotgun sequence.
ACCESSION   DASQKS010000164 DASQKS010000000
VERSION     DASQKS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5547848"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..714
                     /locus_tag="VE170_04140"
     CDS             64..714
                     /locus_tag="VE170_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYJ14654.1"
                     /translation="MDTLEIQVEARTKQRKRDARRLLRAGKIPAILYGPKMAPVTLAL
                     DKKEFSRRVAGLQGSHLVRMKSAAAELAEKVALVKDMQFHPITDDVIHADFYEVDLSA
                     RIQVHVPLHFVGKAVGIVNGGILQPILREIEVECLPLDIPEFFDVDVSTLDIGDSVHI
                     EDLHMPEGVVSMAEDNLALVTVVPPTVEAEAATVAPAEGVVAAPADAKVDAKKDNP"
     gene            751..1335
                     /gene="pth"
                     /locus_tag="VE170_04145"
     CDS             751..1335
                     /gene="pth"
                     /locus_tag="VE170_04145"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYJ14655.1"
                     /translation="MKIVVGLGNPGSTYVGTRHNIGFRVIDRLARQQNIALDQQRCAA
                     LIGLGKIHEEPLLLAKPQTFMNRSGVAVAALLKEYGLTAADLVVIYDDLDLPLGRIRI
                     RTQGTAGGHLGVSSIIEHLGGVPFNRIRVGIGRPSEGNAVIDYVLAPFDEAEVGELSA
                     VIERSAAALGCLIREGAPAAMGVYNRAVDGSGTL"
     gene            1371..1817
                     /gene="rpsF"
                     /locus_tag="VE170_04150"
     CDS             1371..1817
                     /gene="rpsF"
                     /locus_tag="VE170_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYJ14656.1"
                     /translation="MEGINTVTLYETLFVVHPEKGPRNKEFIERFNKVVEGQEGTIVQ
                     VEEWGLRDLAYRIEKQGKGFYTLMRYKATGKAVAELERNLKLTDGILRYLTTRAVEDS
                     PMTSPSAPRTYSDEPPRRNYDDRRNYDDRRNYDDNRSRGGDREPSA"
     gene            1975..2268
                     /gene="rpsR"
                     /locus_tag="VE170_04155"
     CDS             1975..2268
                     /gene="rpsR"
                     /locus_tag="VE170_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HYJ14657.1"
                     /translation="MAQYERSSRPSRERSDEDKGGQRRRPMFRRKVCRFCADKSLRID
                     YKDLRTISSFVSERGKMTPSRITGNCAHHQRLLTTAVKRARSIALLPFTTANN"
     gene            2292..3245
                     /locus_tag="VE170_04160"
     CDS             2292..3245
                     /locus_tag="VE170_04160"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HYJ14658.1"
                     /translation="MQRLEVITRFILALALSVFLFMSSISLPPVGALLLPFVAQPVLL
                     FGVKYGIGFGLGVVLVALLLLAVFGGAELSLLYGVFAVLAAVLLALLGRLRKVEYLVV
                     GAATAMFAWALGVARYFFGSWAVLAEDFRTGLSQQFATATRMQEQLGFSRESLDLVKE
                     RMPQLIDMTLQLLPGLLFLTFAIVALINLLSLRRRFPEHRSAWFAIENFRDWKAPEPL
                     VWGLIASGFSLFFPVAAIARLIAMNLLLIVGAFYFAQGLAVIAFFFHKNNVPRFLRGI
                     TYILIVFQQIFTLMVIGLGLFDLWGDFRRLGKGNLTPSQAS"
     gene            3256..3708
                     /gene="rplI"
                     /locus_tag="VE170_04165"
     CDS             3256..3708
                     /gene="rplI"
                     /locus_tag="VE170_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYJ14659.1"
                     /translation="MEVILKEDIANVGKIGEVVRVRDGYARNFLLPRGLVLEANKKNL
                     KTFEHAKKIIADQKSKVLRQAQAVGDELAGVSLVIPMKVGEEGKLFGSVTNMQIEKAL
                     KAKGLVVDRRKIHLNETIKVAGDYDVPVRLSADLTVTLKVSVVSDSKD"
     gene            3852..4745
                     /locus_tag="VE170_04170"
     CDS             3852..4745
                     /locus_tag="VE170_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYJ14660.1"
                     /translation="MTAIAPPLPSLFGIERGRLLILAAAILWSLAGVFIKFLDIHPLA
                     IVVYRSLFAALVFTPFLQRRDFRCNGTIVVSVLSYTAAISAFVSANKLTSAANAIVLQ
                     YTAPIFVFLFSWLVRREKIANANLFALAVAMLGIAIISLDSAGEPEMAGVSLALVSGV
                     LFAAFMINLQRARDISPVYLTWINNSVCALLLVWIVRSQLVLTSHQLTILAVMGAVQL
                     GAPYFLFSKGLQTVSLPEAALIALIEPVLNPLWVALIVGELPSAATVWGGALILFGLG
                     VRYLWPLIGAPAEAEGDNAAN"
     gene            complement(4754..5173)
                     /locus_tag="VE170_04175"
     CDS             complement(4754..5173)
                     /locus_tag="VE170_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14661.1"
                     /translation="MPIEIIDMVKKAGEGKRQVIFNTKKFHAWVHVYPKPGDADDMHC
                     HNADQTFYVIDGECTMHFPDGGKAVMTPGKVATVTGGSFYQLENTGSGPMVLMGNRSG
                     PSEAIQHINYELRKDIKTLPKEEMDKIRHPGNVRISE"
     gene            5297..6112
                     /gene="lsrF"
                     /locus_tag="VE170_04180"
     CDS             5297..6112
                     /gene="lsrF"
                     /locus_tag="VE170_04180"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HYJ14662.1"
                     /translation="MEACMSMHWGMANRLGRIIKPDGRAVMLAVDHGYFLGPTSGLED
                     PRKTIEPLMPHADSIMLTRGVLRTSVDAKSDKPIVLRVSGGTSILKELSNEDITVSIE
                     DCIRLNVSAIALSIFVGSDFEKETLISLAKLVDEGEKYGIPVLAVTAVGKDMARDVRY
                     LGLSCRIAAEMGAHIVKTYYCDDFDELIGSCPIPVIVAGGKKTPEMEALELAHNAVSH
                     GAVGVDMGRNIFQSDSPVGMIKAIRAIVHENVSVKEAFKIYQSETSSNARKAS"
     gene            6165..7190
                     /locus_tag="VE170_04185"
     CDS             6165..7190
                     /locus_tag="VE170_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYJ14663.1"
                     /translation="MRVAMYYSNRDVRLEELPVPKIGAGELLLRTRASGICGSDLMEW
                     YRIKKVPLVLGHEITAEVAEVGESVEGFSVGDRVFSTHHVPCGKCRYCLAGHQSVCEL
                     LRTTHFEPGGFAEYIRVPKINVELGTLRLPDSMTFDEGSFIEPMACVVRAQRFAKLAA
                     GQTVLVIGSGISGLLHVQLAKARGAARVVATDISDYRLNAAKQFGADATIHGAEDVPA
                     RLKEFNDGRLADLVIVCTGAMPAIQQATKSIDRGGTLLFFAPTAAGVDAPIPLFDFWR
                     DEITVVTSYAGSGDDLTESLALIRDHKVRVADMVTHRLPLAETGVGFQLTASGLDSIK
                     VIIDPLL"
     gene            complement(7227..8216)
                     /locus_tag="VE170_04190"
     CDS             complement(7227..8216)
                     /locus_tag="VE170_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14664.1"
                     /translation="MCAVKFAPLTLILLFFAATLEPCAHAQTLKKVRMASSSTNVSFL
                     ALYTALHRGFFKDEGIDLEIIFMPANLASTAVLNGDVDFNGAVTGTIGAAVQGRPMKV
                     LLFTVSKPLLFLIGQKNIKDLKQLKGKKIAGSSPGGSATLLANQALKQIGLEPGKDVS
                     VLQMSGNAASRLAVLESGVVEASLISVPENIIAQEKGYNELLFLGDVIEFPQNGFGTS
                     DKRIRENPDEVYRMVRATLRGLQFVWDKNNQEAVTNILMKQWKVNDRKMAAEMSRQVG
                     RVLTKDAYVKPESVQVLIDLVRESAKVTKPVTVADVVDYSFLDKARKELGIVK"
     gene            complement(8239..>9864)
                     /locus_tag="VE170_04195"
     CDS             complement(8239..>9864)
                     /locus_tag="VE170_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ14665.1"
                     /translation="TKMCFVYLSPATKYTLGRGATSGYFGTSLKCAGYDGIIVTGAAQ
                     TPKYLYIGEDKVELRDAGRVWGKGIRETEDTLRNESGHQDARVGAIGPAGENLVRAAM
                     LVNDYNHTAAHGLGAVMGSKKLKALVAWGTKRPRLHDKTRLIDAGVRWSKAIASRKTT
                     VKKRLTSVGHGEDWGAITKLNWRSTIITDEAKGLEQNRVTLRPCFQCPRMCPWDVEIG
                     EGRFKGKVGHFNAGSEWMDTFYNLGFKGNDVLYLSERINDLGIECSHFACGAGLAFEA
                     WEKGVLGPDKTDGLKLEWGDMDAVESLLERCARRETWLGNILADGPKELAEELGGEAK
                     NWVVHTKGGTPAQHEWRPMLSQMLRELVASGGMKPQGAGGTEPPADLKYREKWGPLDA
                     SQPDGWTSSHLITEKVRQACGIMGGCWFALNDKPADGVQSMLDALNATTGWDVSLDEL
                     VDAGHRAIILQSVFGTQRGWIAEHDWQDVGPRFLEPIPDGKYKGFTIAKWLPELVYEY
                     YKISGRHEHTGRPYLDTLQKLGLEEFKEWSQLD"
BASE COUNT         1937 a         2661 c         2922 g         2344 t
ORIGIN      
        1 cgccgcaccc atgaagaaga gtcgatcagc tcgctgttcg tttaatcaca ggaggtagtt
       61 cccttggata ctctggagat acaggttgaa gcccgcacta agcaacgtaa acgtgacgcc
      121 agacgattgc tccgcgccgg caagatcccg gcaattctat acggtccgaa gatggcgccg
      181 gtcaccttgg cgctcgacaa gaaagaattc tctcggcgcg ttgcgggact gcaaggttcc
      241 cacctcgtgc ggatgaaatc ggcggcggcc gagctcgctg aaaaggttgc cctggtgaag
      301 gacatgcaat ttcatccgat caccgacgac gtgatccatg ccgatttcta cgaagttgat
      361 ctttccgcca ggattcaggt ccacgtgccg cttcacttcg tcggtaaggc ggtcggtatc
      421 gtcaatggcg gtattcttca gccgatcttg cgcgagatcg aggtcgagtg tttgccgctc
      481 gatattcccg aattcttcga cgttgacgtc tccacgctgg acatcggcga ttcggtgcat
      541 attgaagatc tgcacatgcc tgagggtgtc gtgtcgatgg ccgaagacaa cctggcgctg
      601 gttacagtcg tgccgcccac tgttgaagcc gaagcggcga cagtcgcacc cgccgaaggt
      661 gtcgtcgctg cgccggcgga cgctaaagtg gacgccaaaa aggataatcc ataagcaatt
      721 ggttatggcg ctgtcgtcga aattggccgt gtgaaaatcg tcgttggctt agggaatccg
      781 ggaagcacgt acgtgggcac acggcataat atcggattcc gagttatcga ccgcttggcg
      841 cggcagcaga acatcgcgct ggatcagcaa cgatgcgccg cgctgattgg actgggaaaa
      901 attcacgaag aaccgttgct cttggccaag ccgcagactt tcatgaaccg cagcggcgtg
      961 gcggtggctg cgctgcttaa agagtatgga ctaacagccg cggatttggt ggtcatctac
     1021 gatgaccttg atttgcctct gggccggata cggattcgta cccagggtac tgccggcggg
     1081 catctcggtg tcagctcgat catcgagcat ttaggcggcg tgccgtttaa tcgtatccgc
     1141 gtcggtattg gccgcccgtc cgaaggaaac gcggtgatcg actatgtgct cgcgcccttc
     1201 gatgaagctg aagtgggtga attgtctgct gttattgaac gctcagcggc cgccctgggt
     1261 tgcctgattc gtgagggcgc gccggcggcc atgggtgttt ataatcgagc ggttgacgga
     1321 agcggcacat tgtaaacgcc aagtgcttat gttagataga gaattttcct gtggagggta
     1381 ttaatacggt aacattatac gaaactcttt ttgtcgtgca tcctgaaaaa gggccgcgca
     1441 ataaagaatt tattgaacga ttcaacaagg tcgtcgaagg ccaagaaggc accatcgtgc
     1501 aggtcgaaga atggggactg cgcgatctcg cttatcgcat cgagaaacaa ggtaagggtt
     1561 tctatacctt gatgcgctac aaggccaccg gcaaggcggt tgcagaactc gagcgtaacc
     1621 tcaaactcac ggacggtatt cttcgctacc tcacgacgcg agccgtggaa gacagcccga
     1681 tgaccagccc gtcggcgccc cggacctata gcgacgaacc gccgcggcgc aactacgacg
     1741 atagaagaaa ctacgacgac agacggaact acgacgataa taggtcgcgt gggggcgatc
     1801 gcgaaccctc ggcctagatc ggcgtgcgcc atcccgcggt tcgcgcgagg ttggctttga
     1861 tttgtcgatc attttaattc gccgtagtcg cacggttctc caatggttgg aattccggtt
     1921 ctaagtacag atccgcgact aggaaataag acgaaataag aggtagcgta atttatggca
     1981 cagtacgaac ggagctcaag acctagccgc gagcggtccg atgaagacaa gggtggtcaa
     2041 cgtcgccggc cgatgtttcg gcgcaaagtt tgccgttttt gcgccgacaa aagcctgcgg
     2101 attgattata aggatctgcg gacgatttct tccttcgtca gcgaacgcgg caagatgacg
     2161 ccgagccgga tcaccggcaa ttgcgcgcac catcagcgat tgctgacgac cgcggtcaag
     2221 cgggcgcgca gcattgcctt gctgccgttt actaccgcca acaactaaat tcgtttctct
     2281 ggccaacacc catgcagcgg ctagaagtca ttacccggtt tatattggct ctggccctga
     2341 gcgtgttttt gttcatgagc agcataagtt tgccgcccgt cggcgcgcta ttgctgccgt
     2401 ttgtcgctca accagtgctg ttgtttggcg ttaaatacgg cattgggttt ggactcggcg
     2461 tggtgctcgt cgcgcttttg ctattggcgg tctttggcgg cgccgagttg agtctgctat
     2521 acggcgtatt cgcggtcctg gcggcggtgc tgttggcgtt gctcgggcgg ctgcgtaaag
     2581 ttgagtatct tgtcgtcgga gcggccaccg caatgtttgc ttgggcgctc ggcgtggccc
     2641 gttatttttt cggctcttgg gcggtgctgg cagaagattt ccgcacggga ttgagccaac
     2701 agttcgcgac cgcgacgcgc atgcaggaac agctaggctt ctccagagaa agcttggacc
     2761 tggtaaaaga aagaatgcca cagctgattg atatgacttt acagcttctc ccggggttgc
     2821 tgtttttgac ctttgcgatc gtcgccttga tcaatcttct ttcgctgagg cgccgtttcc
     2881 ccgagcaccg aagcgcatgg tttgcgatcg agaatttccg cgattggaag gcgccggaac
     2941 cgctggtctg gggtctaatc gcgagcggct ttagcttgtt tttcccggtt gccgcgatcg
     3001 cgcgtctgat tgctatgaat ctgttgttga tcgtcggggc tttttacttt gcccaggggt
     3061 tggcggtgat cgcgtttttt tttcacaaga acaatgtacc gcggttcctg cgcggcatta
     3121 cctatatctt gatcgtattt caacaaatct ttacgctgat ggttattggg ctggggctgt
     3181 tcgacctatg gggcgatttt cgccgcttgg gtaaaggcaa cctcacgcca agccaagcgt
     3241 cctagcggag gaaccatgga agttattctc aaagaagata tcgccaacgt cggcaagatc
     3301 ggcgaagtcg tgcgcgtgcg cgacggttat gcgcggaatt ttttgctgcc gcggggtttg
     3361 gtgctggaag ccaacaagaa aaatttgaag accttcgagc atgcgaaaaa aatcatcgcc
     3421 gatcagaaaa gcaaggtgct gcgccaggcc caagccgtgg gcgacgaatt ggccggggtg
     3481 tcgctggtca ttccgatgaa ggtgggcgag gaaggcaagc tcttcggttc ggtgaccaac
     3541 atgcagatcg aaaaggcgct caaggccaaa ggtctggttg tcgaccggcg caagattcat
     3601 ttgaacgaaa ccatcaaggt cgcgggggat tacgacgtgc ccgtccggtt gtccgcggat
     3661 cttacggtaa ccctcaaagt gtcggtggtg tccgacagca aagactaagc gattcggtaa
     3721 tacattccaa ttttttgatc ataatggggt ggcgcatccg agtgccgccc catttttctt
     3781 ggcgcgggtt ggttgcggca agcatgctgg aaatcgaatc cagccgaata ggctgcgctc
     3841 agtatccgaa aatgaccgcc atagcacccc cactgccatc cttattcggc atcgagcgcg
     3901 gccggctgct gatcctcgct gccgccattc tctggagtct cgccggggtt tttattaaat
     3961 tcctcgatat ccatccgcta gctatcgtag tttaccgcag tttgttcgct gcgctggtat
     4021 tcacgccgtt tttgcagcgg cgtgattttc gctgcaacgg cacgatagtg gtttcagtct
     4081 tgagttatac cgcggcgatc agcgcgtttg tctcggccaa taaactgacc agcgcggcga
     4141 atgccatcgt gcttcaatat acggccccga tctttgtgtt tttgttttcg tggctggttc
     4201 ggcgggaaaa aatcgccaat gccaatctat ttgccctggc ggtggcgatg ctcggtatcg
     4261 ccatcatatc actcgacagc gccggcgagc ccgagatggc gggggtctcg ctcgctctgg
     4321 tgagcggtgt gctgttcgct gctttcatga tcaatttgca gcgcgccaga gacatcagtc
     4381 cggtctattt gacctggatc aacaatagtg tttgcgcgct tttattggtc tggattgttc
     4441 gatcgcaact ggtgctgacg tcgcatcaat tgaccattct cgctgtcatg ggggcggtgc
     4501 agctcggtgc gccctatttt ctattttcca agggactgca gacggtgtca ttgccggaag
     4561 cagcgttgat cgcgctgatc gagccggtgc tcaatcctct atgggtcgca ctaatagtcg
     4621 gcgagttacc gtcagccgcc actgtttggg gcggcgcgtt gatcctgttc ggcctcggcg
     4681 tgcgctatct ttggccgttg atcggcgcgc ctgccgaggc cgaaggggac aatgcggcga
     4741 attgagggct ggttcattcg gagatgcgca cgttgccggg atgacggatt ttatccatct
     4801 cttctttcgg tagcgtcttg atgtccttgc gcagctcgta attgatatgc tggatcgctt
     4861 ccgatggccc ggagcggttg cccatgagca ccatcggacc gctgccggta ttctctagct
     4921 gatagaacga cccgccggtg actgtagcca ccttacccgg tgtcatgact gccttgccgc
     4981 cgtcgggaaa atgcatggtg cattcaccgt cgattacata gaacgtttgg tcggcgttgt
     5041 ggcagtgcat atcgtcggcg tcgccgggct tggggtaaac atgcacccag gcgtgaaact
     5101 tcttggtatt gaagatcact tggcgtttgc cttcgcccgc cttcttgacc atatcgatga
     5161 tttcaatcgg cataattcct ccctaggagt tgttcccgtc aagttatagc tgtgcctttg
     5221 cgctgtcaaa taccacagcg gcgaggattc acattcaaaa acgatttgtg atagcttttg
     5281 gccaatcaat cttgcaatgg aggcatgtat gtcgatgcac tggggaatgg cgaatcgatt
     5341 gggacgaatc atcaaaccgg atgggcgggc ggtgatgctt gccgtggatc acggctattt
     5401 tctcggaccg accagcggtt tggaagatcc gcgtaaaacc atcgaaccgt tgatgccgca
     5461 cgccgattcg atcatgctaa cgcgcggcgt cctgcgcacg tcggtggatg ccaagtcgga
     5521 caagccgatc gtgctgcgcg tctcgggtgg cacgagcatt ttgaaagaat tgtccaacga
     5581 agatattacc gtatcgatcg aggactgcat ccgcttgaac gtctcggcga tcgccctgtc
     5641 gatcttcgtc ggttccgact tcgaaaagga aaccctgatc agtctcgcca agctggtcga
     5701 cgaaggcgag aagtacggca tccccgtgct cgccgttacg gcggtcggca aagacatggc
     5761 gcgcgatgtg cgttatctgg gtctgtcctg ccgcatcgcc gcggaaatgg gcgcgcatat
     5821 cgtcaagact tactactgcg acgatttcga cgaactgatc ggcagctgcc ccatccccgt
     5881 gatcgttgcc ggcggcaaga agacgcccga gatggaagct ctcgagctcg ctcacaatgc
     5941 cgtcagccac ggcgccgtcg gcgtcgacat gggccgcaat atttttcagt ctgactctcc
     6001 cgtgggcatg atcaaggcca tccgcgccat tgtccatgaa aacgtttcgg taaaagaggc
     6061 gtttaaaatt tatcagagtg agacgagtag caacgcgcgc aaagcgtcct gaattttcca
     6121 ttcattgacg gcgagacctg aactcgattt ccaggacctc acagatgcgt gtcgccatgt
     6181 attacagcaa ccgtgacgtt cggttggaag agttgccagt gccgaaaatc ggcgccggcg
     6241 aactcttgct ccggacccgc gcgagcggga tttgcggcag cgatctgatg gaatggtacc
     6301 gaatcaagaa agtgccgctg gtcctgggcc atgaaattac tgccgaagtc gccgaggtcg
     6361 gtgaaagtgt ggaaggtttt tctgtcggcg atcgggtctt ttctacgcac catgtccctt
     6421 gcggcaaatg ccgctactgc ctagcgggtc atcaatcggt gtgtgagttg ctgcgcacga
     6481 ctcatttcga gcccggcggg tttgccgagt atattcgcgt gcccaagatc aacgtcgagt
     6541 tgggtacgtt acgccttccc gattcgatga ctttcgacga gggctcgttc atcgaaccta
     6601 tggcgtgcgt cgtgcgggct cagcgattcg ccaagctagc ggccggccag accgtgttgg
     6661 tgatcggcag cggtatttca ggcttgctgc acgttcagct cgccaaggcg cgcggcgcgg
     6721 ctcgcgttgt cgccaccgat atcagcgact atcgattgaa tgcggccaaa cagttcggcg
     6781 ccgatgccac gattcacggc gcggaagacg tgccggcgcg gctaaaagaa ttcaacgacg
     6841 gccgcctggc cgacctggtg atcgtttgca cgggcgccat gccggcgatt cagcaggcga
     6901 cgaaatcgat cgatcgcggc gggacgttgc ttttctttgc gcccaccgcg gcgggcgtcg
     6961 atgcgccaat tccgctgttc gatttctggc gcgatgaaat caccgtggtt acctcgtacg
     7021 ccggcagtgg cgacgatttg acagagtccc tggcattgat tcgcgaccat aaggtgcggg
     7081 ttgccgacat ggtgacgcac cgattgccgc tggcggagac aggtgtgggt tttcaactga
     7141 cggcgagtgg gttggattcg atcaaggtga ttatcgatcc gttgctttga ttactgctac
     7201 agcggacgca gcatgcgccg cagcggttat tttacgatgc ccaattcctt gcgcgcctta
     7261 tccagaaaac tgtagtcgac gacatcggca acggtcacgg gtttggtcac cttggcgctc
     7321 tcgcggacaa ggtcgatgag gacttggacc gattccggtt taacgtaggc gtctttggtc
     7381 agcacacgac cgacttggcg cgacatttcg gccgccatct tccggtcatt cactttccat
     7441 tgtttcatca aaatgttggt gaccgcttcc tggttgtttt tatcccagac gaattgcagg
     7501 ccgcgcaacg tggcgcgtac catgcggtaa acttcgtcag ggttttcgcg gatgcgtttg
     7561 tccgaagtgc cgaagccatt ctggggaaat tcgatgacgt cgccgagaaa aagtagttcg
     7621 ttataaccct tttcttgggc gatgatgttt tccggcacgg atatcagcga tgcctcaacg
     7681 accccggatt ccaatacggc gagccggctc gcggcgttgc cggacatttg caggaccgaa
     7741 acgtccttgc cgggctccag tccgatttgt ttgagcgcct gattggcgag tagcgttgcc
     7801 gagccgccgg gcgaactgcc ggcgattttc ttgcctttga gctgcttcaa gtccttgatg
     7861 tttttctggc cgattaaaaa tagcagtggt ttagacacgg tgaagagcaa gactttcatc
     7921 ggccggcctt ggactgcggc gccgatggtg ccggtgacgg cgccgttgaa gtcgacgtcg
     7981 ccgttcagta ccgccgtgct cgctaagttg gccggcataa aaatgatctc caggtcgatg
     8041 ccttcgtctt taaaaaagcc gcgatggagc gcagtataga gggcgaggaa tgagacgttg
     8101 gtcgacgagc tcgccatgcg gacttttttc aatgtctgcg catgggcgca cggttccaga
     8161 gttgcggcaa aaaataataa gatcaacgtt agaggtgcaa atttaactgc gcacatttta
     8221 gtctcctggt ggatcgagtt agtcaagctg gctccactct ttgaactctt cgagtcccaa
     8281 tttttgcagt gtgtccaggt atggccggcc ggtgtgttcg tggcggccgg agattttgta
     8341 atattcgtaa actaactccg gcagccattt ggcgatggtg aagcctttgt atttgccgtc
     8401 ggggattggt tccaaaaaac gcggtccgac gtcttgccag tcatgttcgg cgatccagcc
     8461 gcgctgggtg ccgaagacgc tctgtaaaat aatcgcacgg tggccggcgt cgacgagttc
     8521 gtccaaactg acgtcccaac cagtggtcgc gttgagtgcg tcgagcatgc tctgcacgcc
     8581 gtcggcaggt ttgtcgttta gcgcgaacca gcagccaccc atgatgccgc aggcctggcg
     8641 caccttctcg gtaattaaat gcgagctggt ccaaccgtca ggttgactgg catcgagcgg
     8701 accccatttc tcccggtatt tcaaatccgc gggcggttcg gtgccgccgg cgccttgagg
     8761 tttcatgccg ccgctggcaa ccaattcgcg gagcatctgg ctcaacatcg ggcgccattc
     8821 atgttgcgcc ggcgtgccgc ccttggtgtg caccacccag ttcttggctt cgccacccag
     8881 ttcttccgcc agctctttgg gaccgtcggc gagaatgttg ccgagccagg tctcgcgccg
     8941 ggcgcagcgt tccagcaagc tctcgaccgc gtccatatcg ccccactcca acttcaagcc
     9001 gtcggttttg tcggggccga gcacgccttt ctcccaggct tcgaaggcga ggccggcacc
     9061 gcaggcgaag tggctgcact cgatgcccaa gtcgttgatg cgctcggaga gatagagcac
     9121 gtcgttgcct ttgaagccga gattgtaaaa cgtatccatc cactcgctgc cggcgttgaa
     9181 atggccgacc ttgcccttga agcggccttc gccgatctcg acgtcccatg ggcacatgcg
     9241 cgggcactgg aagcaagggc gcaaggtcac gcgattttgc tccaaacctt tggcttcgtc
     9301 ggtgatgatg gtgctgcgcc agttcaactt ggtaatcgcg ccccagtcct caccatggcc
     9361 gacgctggtc aaccgtttct tcacggtggt cttgcgcgag gcgatcgctt tggaccagcg
     9421 aacgccggcg tcgatcaatc tggttttgtc atgtaaacgc ggccgtttgg ttccccaggc
     9481 gaccaaggct ttgagttttt tcgagcccat caccgcgccc aagccgtgcg ccgcagtgtg
     9541 attgtagtcg ttgaccagca tggcggcgcg caccaggttt tcaccggccg gaccaatggc
     9601 gccgacgcgc gcgtcttgat ggccggactc gttgcgcagc gtatcttcag tttcgcggat
     9661 gcctttgccc cagactctgc cggcgtcgcg cagctcgact ttgtcctcgc cgatatataa
     9721 atacttgggg gtttgcgccg cgccggtgac gatgatgccg tcgtagccgg cgcattttaa
     9781 cgacgtgccg aaatagccgc tggtggcgcc gcggccgagg gtgtacttgg tcgccgggct
     9841 caaatagacg aaacacatct tggt
//
LOCUS       DASQKS010000165         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4290281, whole genome shotgun sequence.
ACCESSION   DASQKS010000165 DASQKS010000000
VERSION     DASQKS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4290281"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..320
                     /locus_tag="VE170_04200"
                     /pseudo
     CDS             1..320
                     /locus_tag="VE170_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646606.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            523..1587
                     /locus_tag="VE170_04205"
     CDS             523..1587
                     /locus_tag="VE170_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14666.1"
                     /translation="MNLSSNLIEVEDNWEAVNDWFLKEKLSDGLPIVPPTAARVEKML
                     AATSRDPADVIGPIPPKWAPCSIEKIAINAVMAGCLPEYLPVIIAAVEAICEPRFNLY
                     GVQATTGYVGPALLLNGPIRTALNVNCDSGVFGPGFRSNATIGRAIRLILITVGGGYP
                     GDTDRSTFGWPGKYTMCFGENQEKSPWEPYHVEFGFKPEESAVTVFGINGFLPIHTAG
                     NRGEQALASLAEVIKMHHGVSHLDVGSFGGGTPLIALGIEDAEIMARDGITKKQVKDY
                     LWEHASLKFSEIPARRKGHKSDDELMRESINMTPDGVVHLSTKAEDIMVIVIGGKHRH
                     SVFLPMWTGRNTLCVIKQIG"
     gene            1611..2624
                     /locus_tag="VE170_04210"
     CDS             1611..2624
                     /locus_tag="VE170_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14667.1"
                     /translation="MGIRSKIENGRSKIGSALALLIVVILIGVPLAAQDKVRFPIGAS
                     SKTFSYGPLWLGQKLGYFEREGLDVQIVVMRGTPITIQALATESIYVANVGTDGVIGA
                     VDRGLDLAMVGSLLNNLSMSLVAAKPYKSFDDLRGKTIGSQTITTGTGFALRLVLRAH
                     GLEYPRDYQILNVGGVTDRYIALQSGQIAATPLSVPLDISAKQQGFNVLGYFADDLPN
                     YFLNPYTVKRSWAEKNRTLVVRYMKAIVQTHRWLFDNRDAACAYLSKEMSMTVESCRF
                     AWEYSVKTRVWDRNADLNLDGVRTMIKIVAEIAGQKEPLAQPAKYIDQSYLKQAFAEL
                     AKK"
BASE COUNT          606 a          762 c          854 g          625 t
ORIGIN      
        1 ggatttcgcc acgcgatggc gcttaattaa gacggacttc tcgaagcaga ttggcgccgg
       61 cgagccgttc accgagccgc gtaagcaaag gtgagcgggg aatttggcaa cgacggtttt
      121 gggagcacac gctgcgcgat gagaacgatt tcgcccgtca ttgcgattat attcatttca
      181 atccggtgaa acatcggcat gtgtcgcgtg tcagcgcatg gccacattcc tcgttccagc
      241 gatttgcgcg gcttggattg tatcaactcg attggggtag gtgtgctgcg aatgacggtg
      301 cggggtttgg cgagcgctga ttttcggacg atgggtatcg ctgcgctcca cccatcctac
      361 gggattcgga tgagggttga ggcgttatat gccgataccc actgattctt cgtttatagt
      421 cgttccttca gggtcacgcc ctgatcgaca gagtgccgcc atgtttgctc tttttttgca
      481 tgggaaatta gcatatcgat acgttaggag tgtgcagaag ctatgaacct gagctcaaac
      541 ttaatcgaag tcgaagataa ctgggaagcg gtcaatgact ggttcttgaa agagaaactt
      601 agcgatggct tgccgattgt gccgccgacg gcggcgcggg tggaaaagat gcttgccgcg
      661 accagccgcg atccggcaga cgtaatcggg ccgatcccgc ccaagtgggc gccctgttcc
      721 atcgagaaga tcgccatcaa cgccgtcatg gcaggttgtt tgccggaata tctgccggtg
      781 atcattgccg cggtggaagc gatttgcgaa ccgcgtttca atctttacgg cgtgcaggcg
      841 accaccggct acgtcgggcc ggcgctcctg ctcaatgggc cgatccgcac cgcgctcaac
      901 gtcaattgcg actcgggtgt gttcggtccg ggctttcgct ccaatgccac cattggccgg
      961 gcgattcgat tgatcctgat caccgtcggt ggcggttatc ccggcgacac cgaccgttcg
     1021 accttcggct ggccgggcaa gtacaccatg tgcttcggcg aaaatcagga gaaaagtccc
     1081 tgggagccgt atcacgtcga attcggattc aagccggaag agagcgcggt gacggtattc
     1141 ggtatcaacg gctttctacc gatccacacg gccggcaatc gcggcgagca agcgctcgct
     1201 tcgttagccg aagtgatcaa gatgcatcat ggcgtcagcc atctcgatgt cggcagtttc
     1261 ggcggcggca cgccgttgat tgcgttgggc atcgaagacg ccgagatcat ggcgcgcgac
     1321 ggtatcacca agaaacaggt caaggattat ctctgggaac atgcgtcgtt gaaattctcg
     1381 gagattcccg cgcgccgtaa gggccacaag tcggacgacg agttaatgcg cgaatcgatc
     1441 aacatgacac ccgacggcgt cgtgcatctg tccaccaagg ccgaggacat catggtgatc
     1501 gtcattggcg gcaagcaccg ccattcggtg tttttgccga tgtggacggg acgcaacact
     1561 ttgtgtgtga tcaagcagat aggatgattg gactgatgga gtagtgagtg atgggtattc
     1621 ggtcgaagat cgagaatgga agatcgaaga tcggatcggc gctcgcgctg ctcatcgtcg
     1681 tcattctaat cggtgtgcca cttgcggccc aggataaagt tcgctttcct atcggcgctt
     1741 cttcgaaaac cttcagctac gggccgctgt ggttggggca aaagctcggc tacttcgagc
     1801 gcgagggatt ggatgttcag atcgtcgtca tgcgcggcac gccgatcacg attcaagcct
     1861 tggccaccga gtcgatttat gtcgccaatg tcggcaccga tggcgtaatc ggcgccgtcg
     1921 acagaggtct cgacttggcg atggtcggca gcctgctcaa caatttgagc atgagcctgg
     1981 tggccgccaa gccgtacaag agcttcgacg atctgcgcgg caagaccatc ggctcgcaga
     2041 cgattacgac cggaaccggc tttgccttgc gtctggtgct gcgcgcccat ggccttgagt
     2101 atccgcgcga ttaccaaatt ctcaatgtcg gcggcgtgac cgatcgttac atcgcattgc
     2161 agtccggtca gatcgccgcc acgccgttga gcgtgccgct cgatatctcc gccaagcaac
     2221 aggggtttaa cgtgctcggc tatttcgccg acgatttgcc caactatttt ttgaacccgt
     2281 acaccgttaa gcgctcgtgg gctgaaaaaa accgcacctt ggtggtgcgc tacatgaagg
     2341 caatagtgca aacccaccgc tggctgttcg acaaccgcga tgctgcttgc gcctatctgt
     2401 ccaaggaaat gtcgatgacg gtggaaagct gccgcttcgc ctgggaatac agcgtcaaaa
     2461 cccgcgtgtg ggaccgcaac gccgatctga atctcgacgg cgtgcggacg atgatcaaga
     2521 tcgtcgccga aattgccggc cagaaagagc cgctggcgca gccggcgaaa tatatcgatc
     2581 agagttacct gaaacaggcg ttcgcggaat tggcgaagaa gtaacgtttg gccgtgcaag
     2641 ccctcgttcc gaggcaactc ggctggttgt gcgcgagcgt ttttgtaaag cggtcaaaac
     2701 actgccactg accacgacag gcctaccaag ggaggttttg ccgagaccgt tggacgcagt
     2761 gttctcagcg acaattaagt ctcgacggaa cgaaagttct gaggctaatt cggtaaagcg
     2821 gtattctcac ccaggcgaaa tcggcta
//
LOCUS       DASQKS010000166         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1767502, whole genome shotgun sequence.
ACCESSION   DASQKS010000166 DASQKS010000000
VERSION     DASQKS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767502"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VE170_04215"
     CDS             <1..917
                     /locus_tag="VE170_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14668.1"
                     /translation="RREAVMYFPLLPHHGWHRQAGRAGIGASFLIPTLADWQQALDLG
                     GLEAAVVYPTRFMHIGQVGMADFAVDLSRAYNDYLHDRFLRHEPRLKGIAILPMQDVP
                     AAVAELRRVVTDYGMVGAVLPADGLPRPLGHPEFHPIYDEANRLGCMLAVHSQNSLRN
                     NDLFMWRGEAATLAHVWPQMRQFTNFMFSGLLGRLPDLKIAFLEAGCGWVPYLMSKMG
                     NRMGEALPPAKLIERGQIYFQCGEEMTTGRDLELLGDECLFWASDFPHEGIVSMKKAV
                     DEFLDREDIPEAAKRKISYDNPKKLYAL"
     gene            complement(1032..1421)
                     /locus_tag="VE170_04220"
     CDS             complement(1032..1421)
                     /locus_tag="VE170_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14669.1"
                     /translation="MPIEIIDMVDFAHQEKKRKEIFNTPRFHAWMHYYKPGHKDEMHC
                     HNADQTFYVIDGECTMRFPDGGTAVLKPGMAATITGGSFYQLENASDQPMIMMGNRSG
                     PSENIQIIDYVTRKDVRQKVQEEFAKA"
     gene            complement(1612..1992)
                     /locus_tag="VE170_04225"
     CDS             complement(1612..1992)
                     /locus_tag="VE170_04225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ14670.1"
                     /translation="MAKRKSIHIPGMEHGAPIPNGVVIGNMVFSSAIGGKDAKTGTMS
                     DDPDEQAEAMFRNLRLFMETVGGSPDNIAYMKVYLKDEKYRDNVNGPWEKMFPDAHDR
                     PARHALKVDLRGKNLFQIEVTAVL"
     gene            complement(2182..2976)
                     /locus_tag="VE170_04230"
     CDS             complement(2182..2976)
                     /locus_tag="VE170_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYJ14671.1"
                     /translation="MPSQSTKVFVSYSHADVSLVAPVVQLLRVNKSLVFQDVDGIQPG
                     KRWRNEIANALSQSHLVVVFWCDHARRSDEVSKEWNAAIEQDKDLLPVLLDATPLPSS
                     LDEFQWIDFRGTVGANHSSMGSSDNDGNSSALSKQSRAPSKAARWLGGLATGFASAVA
                     LSLYILYPRQAPQSTPQPELPAPMLLPAFDFVFSILLLLGVVLVVTAGLAWLSRRRSK
                     HEAAVGKPSPQAGALERHIASEVEAEILRRTAMRRDGGMSPVARSP"
BASE COUNT          659 a          878 c          889 g          721 t
ORIGIN      
        1 cgcggcgtga ggcggtgatg tatttcccgt tgttgccgca tcatggctgg caccggcaag
       61 cgggccgggc ggggatcggc gcgtcattct tgatccccac cctggcggat tggcagcagg
      121 cgttggatct cggcgggctc gaagccgcgg tggtttatcc cacccgcttc atgcatatcg
      181 gccaggtcgg catggccgac ttcgcggtgg acctgagccg ggcctacaac gactatctgc
      241 acgatcgctt cctgcgccac gagccgcgct tgaaaggcat cgccatcttg ccgatgcaag
      301 atgtcccggc cgcggtcgca gagctgcgcc gtgtcgtcac ggactatggc atggtcggtg
      361 cggtgctgcc cgccgacggc ttgccgcggc ccttgggcca cccggagttt catccgatct
      421 atgacgaggc caaccgcttg ggctgcatgc tcgcggtgca ttcgcagaat tcattgcgca
      481 acaacgatct gtttatgtgg cgcggtgagg cggcgaccct ggcccatgtc tggccgcaga
      541 tgcgccagtt caccaacttc atgttttccg gattgctcgg ccggctgccg gatttgaaaa
      601 tagctttctt ggaagccggc tgcggctggg tgccctattt gatgagtaaa atgggtaacc
      661 gtatgggcga ggcgctgccg ccagcaaagc tgatcgagcg cggccagatt tattttcaat
      721 gcggcgaaga gatgaccacc ggccgggatt tagaattgct cggcgacgag tgtttattct
      781 gggcctcaga ctttccccac gaaggcatcg tcagcatgaa aaaagccgtc gatgagttct
      841 tagaccgcga agatattccc gaagccgcga aacgcaagat cagttacgac aatccgaaga
      901 agctctacgc tctctaagct caaactcgtt caaacagttt aaaatgttaa agccgttcaa
      961 gacgtcgtcg gaatcgaaac tccgaaaacg acttgagcgg cttgaactat tttaacgttt
     1021 tgaacgagtt cttatgcttt cgcgaactct tcttgaacct tctgccggac atctttgcgc
     1081 gtgacgtaat cgatgatctg gatattctcc gacgggccgg agcggttgcc catcatgatc
     1141 atcggttggt cgctggcatt ttccagctga tagaaggagc cgccggtgat ggtcgcggcc
     1201 atgccgggtt tgagcacggc tgtgccgccg tcggggaagc gcatcgtgca ctcgccgtcg
     1261 atgacgtaaa aagtttggtc ggcattgtgg cagtgcatct cgtccttgtg gccgggtttg
     1321 taatagtgca tccaggcgtg gaagcgcggc gtgttgaaaa tttctttgcg ctttttttcc
     1381 tgatgggcga aatcgaccat gtcgataatt tcgatgggca taacctatcc tcctaatgtt
     1441 tttagaactt gcaactcatt gtgttgtgcg aagcgcacta ccaatctaac ccttgttggt
     1501 tcaaacgttc aagcagttca agtcgtccaa ctcgcctgac aatctcccga ttccgatatc
     1561 gttttgaacg tgttgaacga ttggaacaac ttgaacggcg cgcgcggcgc gctaaagcac
     1621 cgcggttact tcgatttgaa acaaattctt gccgcgcagg tcgactttga gcgcgtggcg
     1681 ggccgggcgg tcgtgggcat cgggaaacat tttctcccag ggaccgttga cgttgtcgcg
     1741 atatttttcg tctttcaaat agactttcat gtaagcgatg ttatcgggcg agccgccgac
     1801 cgtttccatg aaaagtcgta ggttacggaa catcgcttcg gcctgttcgt cgggatcatc
     1861 cgacatcgtg ccggtcttgg cgtccttgcc gccgatcgcg gacgagaaaa ccatgttgcc
     1921 gatcacgacg ccgttgggga tcggcgcgcc atgttccatg ccgggaatat gaatgctttt
     1981 gcgttttgcc atatttcacc tccttgtcgg aatcgttgcc cttctcatag cacgacgcca
     2041 aatgtcggtc taccgggctg ggacttcact ggcgctgtga aaacatcggt gagataatgc
     2101 cgcggtcgaa agcgaacagg ccgattggag atcatcccag tcgcagaacg tcgactgata
     2161 gatggtcgtg acgaccgagc ttcagggact cctggctacc ggtgacattc cgccatcgcg
     2221 cctcattgcc gtgcgtcgca gtatctccgc ctcaacctca ctcgcgatgt gtcgctcgag
     2281 cgcgccggcc tgcgggctcg gcttgcccac cgctgcttca tgcttcgaac gccgtcgcga
     2341 caaccacgca agacctgcgg ttacgacgag aacaacgcct agcaaaagta ggatactgaa
     2401 cacgaaatcg aaagccggaa gcagcatagg cgcgggtagt tctggttgtg gcgtcgattg
     2461 gggcgcctga cggggataca agatgtacag gctcaacgcg accgcgcttg cgaaacccgt
     2521 cgcgagtcca ccaagccaac gagcggcctt tgacggagct cttgactgct tcgatagcgc
     2581 gcttgaatta ccgtcattat ccgacgaacc catcgagcta tggttggcgc ccaccgttcc
     2641 gcgaaagtcg atccactgaa actcatccaa cgatgagggg agcggcgttg catccagcag
     2701 aaccggcaag agatccttgt cttgttcgat agccgcattc cactccttgg agacttcatc
     2761 tgaccggcgc gcatgatcgc accagaatac gacaaccaaa tgggattgag atagggcgtt
     2821 ggcaatttcg ttgcgccacc gcttacccgg ctgaatgccg tcaacgtcct ggaaaacgag
     2881 agatttatta acgcgcagga gctggacgac cggagccacc agcgacacgt cagcatggct
     2941 ataggataca aagaccttgg tgctctggct aggcatagaa ctgccgacac acccgctacc
     3001 gacgccaaac cgcttaagtt agaatggcta ttgtttattc tttaatcgcc ggccattctc
     3061 tgagtaaccg cccgtacccg ctcatcggat atttcaaatc gagcttggac agcacatacc
     3121 aaaacatcgc cgggttgctg gcgatca
//
LOCUS       DASQKS010000167         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2626552, whole genome shotgun sequence.
ACCESSION   DASQKS010000167 DASQKS010000000
VERSION     DASQKS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626552"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VE170_04235"
     CDS             complement(<1..458)
                     /locus_tag="VE170_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14672.1"
                     /translation="MNLERAFAQANFYAGKTVTVVVGSAPGGLYDIWGRLLARTMGKY
                     IPGNPTMLVQNMPGGGSMIAANYLYGIAKPDGLTFGMFQTHMYLEQMVGRPEVKYDVR
                     KFNWLGSQEKGQMMLYIRADSPYKSIDDIIKSNDTPKCGGSGSADQTALLT"
     gene            complement(561..2249)
                     /locus_tag="VE170_04240"
     CDS             complement(561..2249)
                     /locus_tag="VE170_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14673.1"
                     /translation="MYDLLLKGGRIYDGSGLPSFHGDVAIIEGRIVDIGRLNSGAKRT
                     LKVDDLAVAPGFIDPHTHMDAQLFWDPMGTSSCFHGVTSVVVGNCGLSLAPAKPENRD
                     SMVKSFVRVEAISRRVLEQGFEWQWTSTAEYLRALGTRLGINVGALVGHIAVRHYVMG
                     EDAVERAATPEEIIKMKQLVRQGMEAGAVGFSTNQNPRHIREDLKPVASRLASDEELG
                     SLLDVLGEMNAGVVQLSGGGTDARGRIEYAAALARRTGRPVLWQSISHSWTRPDHWRG
                     MLEATAKVFQEEGLPIYAMTQAKPFEMRYSLLDAQCFDEFPTWKSAMFSPVEVRKQMF
                     GDADCRKKLRVEAIEDKSASVFPRRWDIINVDQVKLAKNKSFERQSVESVAKLQGKDG
                     LDCFLDLSLEEDLETRFSHITTQGDPKAVCEILKHPSVVIGQSDAGAHMGYDARFGYC
                     TAFLGRWVRDHGIMSLEEAVSKLTFRVASIFGLNDRGLLRPGMVADIAVFDPATINTQ
                     EPEYVQDLPGNETRMIQKATGVPFTVVNGTPVIEHGAPTGALPGKVLRPTAWTN"
     gene            2804..>3136
                     /locus_tag="VE170_04245"
     CDS             2804..>3136
                     /locus_tag="VE170_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14674.1"
                     /translation="MFEEHVPQRKIGVLSPLPIIDNAAYEFYQLVPDGVMRVAVSVGL
                     QEFSRADVERVFEPIDQLVAMLTERGVDIIIQSGVPLPILAGREFLQRLLGRIEEAKG
                     KVQVTSTVL"
BASE COUNT          674 a          939 c          849 g          674 t
ORIGIN      
        1 gtcaataacg ccgtctggtc ggcactgccg gagccgccgc acttgggcgt atcattggat
       61 ttgatgatgt catcgatcga cttatacggc gagtcggcgc ggatgtagag catcatctgg
      121 cctttttcct gggaaccgag ccagttgaac ttgcgcacgt cgtacttgac ctctggccgg
      181 ccgaccattt gctcaagata catatgggtc tggaacatgc cgaaggtgag accatccggt
      241 ttggcgatgc cgtaaagata attggccgcg atcatcgaac cgccgccggg catgttctgc
      301 accaacatgg tcggattgcc gggaatatat ttccccatgg tccgagccag tagccgcccc
      361 cagatatcgt aaagaccccc cggcgccgat ccgacgacca cggtgaccgt cttgccggca
      421 taaaagttcg cttgcgcgaa tgcccgttca aggttcaagg ggttcaaggg ttcaaaagta
      481 aagaaaagta agccaaacac gatccgattg aaccgttgaa cccttgaact tttgaacaaa
      541 ttttccatcg caccagcctg ctaattcgtc cacgccgtcg gccgcagcac ctttccaggc
      601 agcgcgccgg tgggcgcgcc gtgctcgatc acgggcgtgc cgttcaccac ggtgaacggc
      661 acgcccgtgg ctttttgaat catgcgcgtt tcattgccag gcaagtcttg gacgtactcc
      721 ggttcctgcg tgttgatcgt cgccggatcg aaaaccgcga tgtcggcgac catgccgggc
      781 cgcaacaagc cgcggtcgtt gagaccgaag atcgaggcga cgcggaaagt cagtttcgac
      841 accgcctctt ccaggctcat gatgccgtga tcgcgcaccc agcggccgag gaatgccgtg
      901 cagtaaccga agcgcgcgtc gtatcccata tgcgcgccgg catccgactg gccaatcacc
      961 accgacgggt gtttaagaat ctcgcacacc gccttgggat cgccctgggt ggtgatatgc
     1021 gagaaacggg tttcgagatc ctcctccagc gacaagtcca agaaacaatc caagccgtct
     1081 ttgccttgaa gcttggcgac cgattcgacc gattgccgtt caaaggattt attcttcgcc
     1141 agcttcacct ggtcgacgtt gataatatcc cagcgccgcg gaaataccga cgccgactta
     1201 tcctcgatcg cctcgacccg cagttttttg cggcaatcgg catcgccgaa catctgcttg
     1261 cgcacttcca ccgggctaaa catcgcgctc ttccaggtgg gaaattcatc gaagcattgg
     1321 gcgtcgagca agctgtaacg catctcgaac ggcttggcct gggtcatggc gtaaatcggc
     1381 aacccttcct cctggaaaac tttcgccgtc gcttcgagca tgccgcgcca atgatctggc
     1441 ctcgtccaac tatgagaaat gctctgccaa agcaccggcc ggccggtgcg ccgggccaat
     1501 gccgcggcat actcgatccg cccgcgcgca tcggtgccgc caccgctcaa ttgcaccact
     1561 ccggcattca tctcgccgag cacgtcaagt aaagaaccta gttcttcatc gctagcgaga
     1621 cggctcgcca ccggctttaa atcttcgcgg atatgacgcg gattctgatt ggtggaaaag
     1681 ccgacggcgc cggcttccat gccttgacgc accagctgtt tcatcttgat gatttcttcc
     1741 ggcgtcgccg cgcgctccac ggcgtcttcg cccatgacat agtggcgcac ggcgatatgg
     1801 ccgaccaagg cgccgacgtt gatccccaac cgcgtgccca gggcgcgcaa atattccgcc
     1861 gtcgaagtcc attgccattc aaagccttgt tcgagcacgc ggcggctaat tgcttcgacg
     1921 cggacaaaac tcttcaccat ggaatcgcgg ttctccggtt tcgccggcgc caagctcaga
     1981 ccgcaattgc cgacgacgac ggaagtcacg ccatggaaac aagacgaagt gcccatcggg
     2041 tcccagaaca gctgagcgtc catatgcgtg tgcggatcga tgaagcccgg cgccaccgcc
     2101 aagtcgtcga ccttcagagt gcgcttggcg ccgctgttca atcgcccgat gtcgacgatc
     2161 ctaccttcga taatggcaac atcaccatgg aaactcggta gccccgagcc gtcataaatc
     2221 cgcccgccct taagcaaaag atcgtacatt gcgcgctcct taattggctt tgcgtcgcag
     2281 ctgatctgcg cgcgtcgatt ttctcatttc cccttgacgg acaaaactta atcgaagtaa
     2341 ccgtctccgg tcaagtagac ctgccatgca tgatacctgc ttcgctggac accggagtga
     2401 tcggataatg gctcgctatt tttctttccc cctttagaaa ccaattatgc gaacactttc
     2461 ctggccgtac tgggagctga caaagtaaat ggctagccat gaccgattcc cccgttggca
     2521 acggggatca agggatttcc tgtggtttgg ggcttcgcag cagatcggca atcctctgcg
     2581 tcccatggaa gaaacaaatt ttaaaggcgg gtgacggaat acttgccgcg tccgcagggg
     2641 ctgatcagaa gttcgtatgc agtgcgtatt ccaaattcat tgaagtagcc acctttgggg
     2701 aaggatcaac gagtagacag agggttacgg tttgaggtaa acagaccgat acgtagcacg
     2761 caactctttt cgcgcctctc gctgtgattt cggaggacag atcatgtttg aagaacatgt
     2821 tccccagcga aaaatcgggg ttctctcgcc gctgccgatc atcgataacg ccgcctatga
     2881 attttatcag ctggtgcccg acggcgtcat gagggtggcg gtctcggtcg gcttgcagga
     2941 attcagccgc gccgatgtgg aacgcgtgtt tgaaccaatc gaccagctgg tcgccatgct
     3001 caccgagcgc ggcgtcgaca tcataatcca gtccggtgta ccgctgccaa tcctggccgg
     3061 ccgggaattc ttgcaacgct tgctaggtcg tatagaagaa gcgaaaggca aagtgcaggt
     3121 aacctcgacg gtgctc
//
LOCUS       DASQKS010000168         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_510036, whole genome shotgun sequence.
ACCESSION   DASQKS010000168 DASQKS010000000
VERSION     DASQKS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510036"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VE170_04250"
     CDS             <1..295
                     /locus_tag="VE170_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ14675.1"
                     /translation="QVGQDGQYVFVVKEDKRVEVRPVTMGSSSEGLAIIAKGLAIGEQ
                     VVREGQFLLGPESRVEIKDQSGAAEKTAAETKKGGANRGKRDKAKAEEKRGAS"
     gene            292..3528
                     /locus_tag="VE170_04255"
     CDS             292..3528
                     /locus_tag="VE170_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ14676.1"
                     /translation="MNISAIFIRRPVTTTLIMIGILIFGTLGYKSLPVSDLPTVDFPT
                     ITVNATLPGASAETMASGVATPLEKNFSTIAGLDSMSSTSSLGRTQITLQFSLSRNVD
                     AAAQDVQAMIARSARDLPPNMPTPPSYRKVNPANNSILLLALTSPTLPLSTVDEFAQT
                     ILAQRISMVSGVAQVDVIGSQKYAVRIQLDPNQLAARQVGIDDVVAAVLRGNVDLPVG
                     TLNAPTRAYTLVSDAQLTNAEAYRPMVVTYRNGAAVRIQDVGTAIDSVENSRVGSWFN
                     DKRAVILGIQRQPGANVVEVVDAVKEILPALRTQIPQSINLEVFFDRTVSIRESVHDV
                     QLTMIGTIFLVILVIFLFLRNVSATIIPSLALPMSIIGTFAVMYLLDYSLDNLSLMAL
                     TLAVGFVVDDAIVVLENIVRHMELGESPYEAAVKGAQEIGFTIVTMTISLVSVFIPVL
                     FMSGMLGRILREFSVTICVAILVSGFISLSLTPMLASRYLRPAHNQKHGWLYHLLGRG
                     LDAMNNGYGSSLGWVMRHHVTTMVVSLLVLVATGYMFGLVPKGFIPSEDTGQLLINTE
                     AAQGVSYEQMVGYQQQLAAIVAKDPNVESFFSSVGVGGVGLTGNTGRIFMKLKARSER
                     PLVCSERAWGKSCQPLNADQLIRELRPKLARVPGIRVSLQNPPVIRVGGRLSRSLYQF
                     TLQSPDAAELYRNAALFEEKIKALPDFQDVGSDLQLKNPQLQVVIDRDKMATMGITAQ
                     GISPRQVEEAFWNAYATRQVSTINTPNNQYQVILELDPKFQNDPSALSMLYIRSTTGQ
                     AATAQPMVPLGSLASLKYTVGPLTVNHSGQLPSVTISFNLREGVALGDGVATINKLAR
                     DNLPATVSTTFQGTAQEFQSSLKSLWVLLALAVFVVYLVLGILYESFIHPLTILSGLP
                     SAGLGALIALYLFNMDLSIYAFVGLIMLIGIVKKNAIMMIDFALDAQRNDGKAPAEAI
                     YEGALVRFRPIMMTTMAAIVGAIPIAVGWGAGAEARQPLGIAVVGGLVISQILTLYIT
                     PVVYLYMERLQIFATGLPARLRGKSGAVPAAGLTPDLSVDNPTGHKVS"
BASE COUNT          770 a         1057 c         1056 g          831 t
ORIGIN      
        1 ccaagtcggc caggacggcc aatatgtttt tgttgtcaaa gaagacaaac gggtcgaagt
       61 cagaccggtg acgatgggca gctcgagtga aggtctagcg atcatcgcta aaggcctggc
      121 catcggtgaa caagtcgtgc gtgaagggca gtttttgctg ggcccggaat cgcgcgtgga
      181 aatcaaggac cagagcggcg cggccgaaaa aaccgcggcg gagacgaaga agggtggcgc
      241 caaccgaggc aagagagaca aagccaaggc ggaagaaaaa agaggagcat catgaacatc
      301 tccgcaatat tcatacgccg tccggtaacc accacgctga tcatgattgg gatactgatc
      361 ttcggtactt tgggttacaa gtcgctgccg gtaagcgatc ttccgacggt ggattttccg
      421 accattacgg tcaacgccac tctgcccggg gcaagcgccg aaaccatggc gtcgggggtg
      481 gcaacgccgc tcgaaaaaaa cttctcgacc attgccggcc tcgattccat gagttcgacc
      541 agcagtctgg gccggacgca aataactctg cagtttagtt tgagccgcaa cgtcgatgcc
      601 gcggctcaag acgtgcaggc gatgatcgcc cgttcggcgc gcgacctgcc gcccaatatg
      661 ccgacgccgc catcttaccg gaaagttaat ccggcgaaca attccatttt gctattggcg
      721 ttgacctcgc cgacactgcc tttgtcgacc gtcgatgaat ttgcccagac gatattagcg
      781 cagcggatct cgatggtgag cggcgttgcc caggtcgatg ttatcggctc gcaaaagtac
      841 gcggtgcgga tccagctcga tcccaatcag ctggccgccc gccaggtggg catcgacgat
      901 gtggtcgccg ccgtgctgcg cggcaacgtc gacctgccgg tgggaacgtt gaatgccccc
      961 acccgtgcct acaccttagt ttcagacgcg cagctgacca atgcggaagc ttaccggccg
     1021 atggtcgtca cctatcgcaa cggcgcggcg gtgcgcattc aagacgtcgg cacggcgatc
     1081 gatagcgtcg agaacagcag agtcggcagc tggttcaacg ataaaagggc cgtcatcctc
     1141 ggaatacagc ggcaaccggg cgccaacgtt gtcgaagtcg tcgatgccgt caaggagatt
     1201 ctgccggctt tgcgcacgca aattccgcag tcgatcaatt tggaggtctt ttttgaccgc
     1261 accgtttcga ttcgggaatc ggtgcacgac gtgcaattga caatgatcgg cacgatcttt
     1321 ctggtcatcc tcgtcatctt cttgttcctg cgcaacgtgt cggccacgat tattcccagc
     1381 ttggccctgc cgatgtcgat catcggcacg tttgccgtaa tgtatctgct cgattacagc
     1441 ctcgacaacc tgtcgctgat ggcgctgacc ctggcggttg gtttcgtcgt cgacgacgcc
     1501 atcgtcgttc tcgaaaacat cgtccgccat atggaactgg gagagtcacc ctacgaagct
     1561 gcggtcaaag gcgcccagga aatcggcttt acgatcgtca cgatgacgat atcgctggtg
     1621 agcgttttca tcccggtgct gtttatgagc ggcatgctcg gacgaatcct gcgggaattt
     1681 tccgtcacta tctgcgtcgc gattctagtt tcgggcttca tctcgttaag tttgactccc
     1741 atgctcgcca gccgttacct ccgtcctgcg cataatcaga agcatggttg gttgtatcat
     1801 ttacttggcc gcggtttgga cgcgatgaat aatggctatg gaagctcact gggctgggtg
     1861 atgcgccacc acgtcacgac tatggtggtc tcgctgctcg tcctggttgc gaccggctat
     1921 atgtttggct tggtgcccaa gggatttatt ccgagcgaag ataccggtca actgctgatc
     1981 aacaccgagg cggcgcaagg tgtttcctat gaacaaatgg tcgggtacca gcagcagctg
     2041 gccgccattg tcgccaagga tcccaatgtc gagtcgtttt tctccagcgt cggcgttggc
     2101 ggtgtgggac tcaccggcaa taccgggcgt atttttatga aactcaaggc gcgctcagag
     2161 cggccactgg tctgctccga gagggcatgg ggtaaatcct gccagccact caatgccgat
     2221 cagttgattc gagaattgcg acccaagctg gcccgtgtgc cgggtattcg cgtttcattg
     2281 cagaacccgc cggtaatccg tgtcggcgga cgactcagcc gcagtcttta ccagtttacc
     2341 ctgcaaagtc cggacgctgc ggaactttac cgcaacgccg cgttattcga agaaaaaatc
     2401 aaagcgttgc cggattttca agatgtcggc agcgatttac agctgaagaa tccgcagttg
     2461 caggtcgtga ttgaccgcga caaaatggcg accatgggga ttacggcgca aggaatttcc
     2521 ccacggcagg tcgaagaggc tttttggaac gcttatgcga cgcggcaagt ctcgacgatc
     2581 aataccccca acaaccaata ccaagtgatt ttggagctgg atccgaaatt tcaaaacgac
     2641 ccatcggcgc tatccatgct ctatatacgc tcgacgaccg gccaggccgc gacggcgcaa
     2701 ccgatggtgc ctttgggttc tctggcgagc ctcaagtaca ccgtgggacc gcttacggtt
     2761 aatcactccg gccagctccc ttcggtgacc atctcgttta acttgcgcga gggggtcgcc
     2821 ttgggagacg gggtggcgac catcaacaag cttgcgcgtg acaatctgcc ggctacggtc
     2881 agcaccactt ttcaaggcac ggctcaggaa tttcaatcgt cgctcaagag cttatgggtt
     2941 ctgttagcgc tcgcggtgtt cgtcgtttac ttggtcttgg ggattctcta tgagagcttt
     3001 atccatccgc taacgatact ttccggccta ccctccgccg gtctcggcgc tttgatcgca
     3061 ctttatcttt ttaatatgga cctgagtatt tatgccttcg tcggcttgat tatgttgatc
     3121 ggtatcgtca agaaaaacgc catcatgatg atcgactttg ccctcgacgc ccagcgcaac
     3181 gacggtaaag cgccggccga ggcgatttac gaaggggcat tggtgcgctt tcggccgatc
     3241 atgatgacca ccatggcggc gatcgtcggc gccattccca tcgcggtcgg ttggggcgcc
     3301 ggcgccgaag cgcgccagcc cctcggtatc gcggtggtcg gcggcttggt catttcgcaa
     3361 atcctaacgc tctacatcac tccggtggtc tatctttaca tggagaggct ccagatattc
     3421 gcaaccggcc tgccggcgcg cttgcgcggc aaatccggcg ctgtgcctgc cgcaggcttg
     3481 acgccggacc tcagcgtcga taaccccacc gggcataaag tttcgtaggc gtcgccggac
     3541 caaaagaatc aacatgacgg gccatcgtct ggtaacaggc gatggccttt cggcgtaggc
     3601 cgcaatggtg ttccatcgtt cacagttaaa tattctttgc tgccggagtg ccaacccgct
     3661 cccatcctgc tccctgggcg atcctcgatg aaacctcgaa gttggcccgt tatc
//
LOCUS       DASQKS010000169         4475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_510431, whole genome shotgun sequence.
ACCESSION   DASQKS010000169 DASQKS010000000
VERSION     DASQKS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510431"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1290..2321)
                     /locus_tag="VE170_04260"
     CDS             complement(1290..2321)
                     /locus_tag="VE170_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14677.1"
                     /translation="MNQKKSATFIWALALALVICAASAGKVFAAAADSLDQLYEKAKK
                     EGKVTLYAPLSPTAMSVIPPAFMKRFPGVAVDHIDATSDKLIARVLAEQRGGRVIADV
                     ISGALTYLPQILDLKIVEHVAIPEAAAYPSQLKGDLWVATDTQYYIAGWNTNLVKKGD
                     EPKSYEELANAKWKDNIMGEPRDYQMLVGFAKRKFNSDEKAIDLFKKIAVNQVEFHRG
                     HSQLTEFLMAGQRAVCFTCYSHHFPVRMKKGAPIQPLMAEGVGEVGGSVMVMKGAPHP
                     SAALLYVRWAVSEEGQKAYAQAGETPAHPKVEPLEKIRPTTPYMLTIDDVREFPKYEK
                     IWKEIFQIR"
     gene            complement(2514..3344)
                     /locus_tag="VE170_04265"
     CDS             complement(2514..3344)
                     /locus_tag="VE170_04265"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ14678.1"
                     /translation="MTGPVVVVTLLILVAGLASAIYRQWRQNRTLKAQLEAVAADLQH
                     LQQSCSRFVPAGVLQHLIGDGVQSGIEAAAPERKVVTVLFADLVGSTAMSGRLEPAVL
                     ARVLNGYFQRMSDAIHEHRGHVAHFLGDGIVAYFGALQPNPWQCNDAVRAALAMRAAI
                     REYNVELGREGLPLLALGIGIDRGLGLVGLVGARERMEYDFVGRTVNVAARVQTLTRI
                     HQADILVTEALSTELDARFVLMPMPAEFVKGISEPVVTYAVREGPSAAAPSAEQPGDR
                     "
     gene            complement(3341..4378)
                     /locus_tag="VE170_04270"
     CDS             complement(3341..4378)
                     /locus_tag="VE170_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14679.1"
                     /translation="MPDPALQSYARRRQGIPHPMIAYFAPVPWMARAVVDMHPEYGLL
                     MHLDQTVADLVVLVVSQENSCRFCYAAVRGLLWAQGMSWARIQRMEQDLTRADLPPRT
                     MAAVAFGRSQSRSGPAGAQAARETLRRSGLGLEELKEIAFTAALTDFHNRAHTIPAIP
                     AYPIESMPDHLYVRLLRPLLGLIVKRYRYRGQPTVLEPVPPYAFARLVETYAGSPIGA
                     ILARTLAEMWASPHLTLRCKLLMIAVIARGLGCAVCAPEVSAALQTEGLKESTLAQVL
                     NHLDAPELDPIERLLVPFARETIWFEPAPLQRRARMLRERLSAPQLLEAIGVASLANG
                     LCRMGATVLDL"
BASE COUNT          932 a         1288 c         1292 g          963 t
ORIGIN      
        1 tatttttatt cttgaactgg acaagcttac cgggcagtta tcacggtggc cttatggctg
       61 agcgaaccca aaaaacaatg acaattgtcc actacaagac atcgctttct attacgacaa
      121 gttagaattt aacgttttct acgctcaacg attttcagaa tgggtgattt atccatgcgc
      181 acgctcataa atttcgttgg cgggatcctc ctagcatcag gaattatcag tgtccttggt
      241 ggactcgttg tgcgcacggt ctaccctagc ggcggcatca tgctctggac aggcggggtt
      301 atgatcctgg aaggcttact gatttgttac ctagccggca cgaaggcctg cccgcgatgc
      361 aacgagaatg tgaaggtcaa agccttgaag tgcaagcatt gcagcacgga gttcgccgac
      421 gccatgtttc aggaattact cccagctgat gccggtgaaa ggtagcaatc ccatgagaac
      481 tgaatacaag ggtacttcaa ttgcaataga gacgaacaac gtgaatggcg gaccctgggc
      541 agcagtcgtt cgggtttggc cggtacacaa ccggattagg gcattgcgcg atcagcgcgt
      601 aatagaaggc agctacaaaa accgggaaga cgcagaagaa gccggcgtgc agtgggctaa
      661 agagcgcatt gacgcatacg ggcaaaatgg acttccatct ctttagattt cagctgcgat
      721 aatagtcact tctgcgcagt tcaaaggccg gacaggcggt gtcagacatt ccagtcatta
      781 caatctaaga cttctctcca cctcccgcgc cggtcgtggg cacaacttag tttcgtaact
      841 acccggacac tacgattcct tgcgcagacc ttcgatgaac gtggaatcaa aaaaactctc
      901 cctttgagct tgccgacagt attgaatcgc gactgtctgg cttctcagat ctcgcccatt
      961 ttctcgaatg ccattccatc aatgtaagcc gtgccctgca atagatttaa atcgctgggg
     1021 acgttaatga cttgctcgac aaaccccgct gaacaccttt tcggtgatcc tctacgccgc
     1081 tgatttttgc gataaatccc gtagtgatga cctggagtca tggggtggac gaaatgtttg
     1141 tctttttgat gctccacctc taagacaaac gttttttctg cggattctca ctattcgatc
     1201 agtcgctctc gtttcccaag catggcctgt gacggctcca ctgccactgg ccggcccgtc
     1261 tgaccattct caggctaatt ggattgattt cagcggatct gaaagatttc cttccaaatt
     1321 ttctcatact tcggaaactc tctgacatcg tcgatggtca gcatgtacgg cgtcgtcggg
     1381 cggatttttt ccaagggttc cactttggga tgagcaggag tttctcccgc ttgagcgtaa
     1441 gccttctgtc cttcttcact gaccgcccaa cgaacgtaga gcagcgccgc gcttggatgg
     1501 ggcgctccct tcattaccat taccgagccg cccacttcgc ccacgccttc ggccatcagc
     1561 ggttgaatcg gcgcgccttt tttcatgcgc acgggaaaat ggtgcgagta gcaggtaaag
     1621 caaacggcgc gctggccggc catgaggaac tcggtcaact gcgaatggcc ccgatgaaac
     1681 tccacttgat tgacggcgat tttcttgaac agatcgatcg ccttttcatc gctgttgaat
     1741 tttcgtttgg caaagccgac cagcatttgg taatcgcgcg gctcgcccat gatgttgtct
     1801 ttccatttcg cgttggcgag ctcttcgtaa ctttttggct cgtcgccttt cttgacgaga
     1861 ttggtgttcc atccggcgat ataatattgc gtgtcggtgg cgacccatag gtcgcctttc
     1921 aactgggacg gatacgccgc tgcctcgggg atggcgacgt gctcgacaat tttcagatcc
     1981 aggatctgcg gtagataggt cagcgctccg cttatgacgt cggcaatgac acgtcccccc
     2041 ctctgctcgg cgagcactcg cgcaatgagc ttatcgctcg tggcgtcgat atgatcgacc
     2101 gcgacgccgg gaaagcgctt catgaacgca ggcgggatga ccgacatcgc cgtcggggac
     2161 agcggcgcat aaagcgtaac ctttccctcc ttttttgctt tctcataaag ttgatcgagg
     2221 gaatctgctg cggcagcgaa aaccttgcct gcggaagcgg cgcagataac cagagccaag
     2281 gcaagcgccc agataaaggt tgcagatttc ttttgattca tttcacttct cctgacgctt
     2341 tgttcgataa ttctaaacca acatctctga aaggttgttc tgtcaccggc cggtatatcg
     2401 gatcacgtta tccggcctct cagatcgccc attttctcaa atcccatgaa agtaagccgt
     2461 gcgctgcaaa agatttatta tacttagtta agtctgacaa ggccgggagc cagtcatctg
     2521 tctcctggct gctcagccga tggagcggcg gccgatggcc cttctcgcac ggcgtaagta
     2581 accacgggct cgctgatgcc tttgacgaat tcggcgggca tcggcatgag cacgaagcgc
     2641 gcgtcgagct cggtacttaa cgcctcggtc acgaggatgt ccgcctgatg gatgcgggtg
     2701 agcgtctgca cgcgcgcggc cacgttcacc gtgcgtccga cgaagtcata ttccatgcgc
     2761 tcgcgcgcgc ccaccaggcc gacgagcccc aaaccgcggt cgatgccgat ccccagagca
     2821 agcaacggca gcccttcccg cccgagctcg acattgtatt cgcggatggc ggcgcgcatc
     2881 gcgagggccg cgcgcacggc atcgttgcac tgccacggat ttggttgcag cgcgccgaaa
     2941 taggcgacga tgccgtcgcc gaggaagtgg gcgacatggc cgcggtgctc gtggatggca
     3001 tcgctcatgc gctggaagta gccgttgagc acgcgcgcca ggacggccgg ctcgagccgg
     3061 ccgctcatcg cggtggaacc gacgagatcg gcgaacaata cggtcaccac tttgcgctcg
     3121 ggagccgccg cctcgatccc cgactgcacg ccgtcgccga tgaggtgctg cagcacaccg
     3181 gctggaacaa agcgcgagca ggattgctgg agatgctgga ggtcggcggc gaccgcttcg
     3241 agctgggcct tcagcgtccg gttctggcgc cattggcgat agatggcgga ggccagtccc
     3301 gccacgagta tcagtagagt gacaacgacg accggtccgg tcataggtcc agcaccgtcg
     3361 cgcccatccg gcacagtcca ttggccagcg aggccacacc gatggcctcg aggagctgcg
     3421 gggccgagag gcgttcgcgc agcatacggg cgcggcgctg tagcggcgcg ggttcgaacc
     3481 agatcgtttc acgagcgaac ggcacgagca gccgctcgat ggggtcgagc tcgggagcgt
     3541 cgagatgatt gaggacttgg gcgagggtcg attccttcaa tccctctgtc tgcagagctg
     3601 cactcacctc cggcgcgcac accgcgcacc ccagtccgcg cgcgatgacg gcgatcatca
     3661 gaagtttaca gcgtagtgtg agatgaggag acgcccacat ctcggcgagc gttcgcgcca
     3721 atatcgcgcc gatcggagag ccggcgtagg tttccacgag ccgggcgaag gcgtagggcg
     3781 gcacgggttc gagcacggtg ggctggccgc gataacgata tcttttgacg atcagaccga
     3841 gcaagggccg gagcagccgt acgtaaagat gatcgggcat gctctcgatc ggatacgcag
     3901 ggatcgccgg gatcgtatgg gcgcggttgt ggaaatccgt caatgccgcc gtgaaggcga
     3961 tttctttcag ttcttcgagc cccaagcccg aacggcgtaa agtttcgcgg gcagcctgag
     4021 ctcctgccgg acccgagcgc gactggctgc gcccgaaggc gacagccgcc atggtccgcg
     4081 gcggcagatc ggctcgcgtc aggtcttgtt ccatgcgctg gatacgcgcc caggacattc
     4141 cctgggccca caacagtccg cggacagcgg catagcagaa acggcacgag ttctcctggc
     4201 tgacgaccag cacgaccaga tccgctacgg tctgatcgag atgcatgagc agcccgtact
     4261 cgggatgcat atcgaccact gcccgcgcca tccaggggac cggcgcgaag taggcgatca
     4321 tcgggtgggg aatcccctgc ctgcgccgcg cgtaggattg taacgccgga tctggcaagg
     4381 gctcgaccag gggcgactcc cattcaacct gttgcaggac accgttcatg atccgactcg
     4441 aaccgctggc tgagcgatcg aaggccgccg ttatt
//
LOCUS       DASQKS010000170         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1768802, whole genome shotgun sequence.
ACCESSION   DASQKS010000170 DASQKS010000000
VERSION     DASQKS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768802"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..469
                     /locus_tag="VE170_04275"
     CDS             131..469
                     /locus_tag="VE170_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14680.1"
                     /translation="MEKPRIRHIAINVEDLDKTAEYYKKVFGMEEKHRRPGGTIYLSD
                     GHMDLALIHSDKYPWGIHHFGFKVDSVKEVEKIANTTAEENTYGAIAESWIKDVEGNR
                     VDVSEHGWPI"
     gene            512..1480
                     /locus_tag="VE170_04280"
     CDS             512..1480
                     /locus_tag="VE170_04280"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14681.1"
                     /translation="MGLFRFRSPWLAFALLVAADGFAGQASSENIKFGTSALSPTMAG
                     VWMAKESGAFERRGINAELVYISSGAVVVQALVGGSLHAALGASNAVIAAILKGAPII
                     AVGSNTSRPGMQLWVHPDIQRPEQLAGKTLGITRFGSTSEFVTRLVLRKLNLEGKTEL
                     RQFGGVIEADIGYRSGQSVGRLSSQSPGPDARKLVDAAELGIPFSMNLLAVSIDFYRK
                     SPKTVEGIIAAYIEGIAALKQRRQQASNVLAKYMGQRGGSADVHYEFVMKYLEAVPRV
                     EPAAIDTVLEMVGSKDAAKAKLYDNSVVDRLVQEGLVDRLYKGAKP"
     gene            1477..2187
                     /locus_tag="VE170_04285"
     CDS             1477..2187
                     /locus_tag="VE170_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ14682.1"
                     /translation="MTEKQWRECLGELVRDYMRSPEMDFYFSIKMNKPRAAVMVQQQS
                     LFVRHRRDCWAHVSANCPVMAVKQRILEHEYEEIIRDDYSDYGHLHLIIRQGKSVGLE
                     PEAIINAEPTPETRAALYAWAWMTCRMAWTASLTGMTITEWCNDDRLLGDLGGGQSTR
                     MAKKWMEDMGFTWKQIPNMQAHSQADEKHSDMFLPFLAEHATGEKEASALQAAKDSLA
                     FNAMYRKGIALAQEKIGL"
BASE COUNT          553 a          609 c          734 g          482 t
ORIGIN      
        1 attgacgatg taaaagaagc gatatatcgc gcgttgactg agcccacacc ttcatctagg
       61 tatgagttcg caggcgcgct aagtgctaaa tctgctatgg aaacagctta agacactcta
      121 aggaggactt atggaaaaac cgagaatcag acatatcgcc atcaacgtcg aggacctcga
      181 caagaccgcc gaatactaca agaaggtatt cggcatggaa gaaaaacacc ggcggcccgg
      241 cggcaccatt tatttatccg atggccatat ggatctggcg ctgattcata gcgacaagta
      301 tccctggggt attcatcatt tcggcttcaa ggtggacagc gtcaaagagg tcgagaagat
      361 cgccaacacc actgccgaag aaaatactta tggcgccatt gccgagagtt ggatcaagga
      421 tgtcgaaggc aaccgggtcg atgtctcgga acatggctgg ccgatataaa tttcgttcaa
      481 ggttcaaggg ttcaaaagtt caaggtttcg gatggggcta ttccgatttc gttcaccgtg
      541 gctagcattt gctttgcttg ttgctgccga tggttttgcc gggcaggcgt caagcgagaa
      601 tattaaattc ggcaccagtg cgttgagccc gacgatggcc ggcgtttgga tggccaagga
      661 gagcggcgcg tttgaacggc gcggcatcaa tgccgagttg gtttacatca gctcgggcgc
      721 ggtggtcgta caggcgctgg tcggcggcag cctgcacgcg gcactgggtg cgagtaatgc
      781 ggtgatcgcg gcgattctca aaggggcgcc gatcatcgcc gtcggcagta acaccagccg
      841 gccaggcatg cagctatggg tgcatccgga tattcagcgg ccggagcaat tggccggaaa
      901 gacgctcggc attacccggt tcggctcgac cagcgagttc gtcacgcgct tggtgctgcg
      961 caagctcaat ctcgaaggca aaaccgaatt acgccagttc ggtggcgtca tcgaagccga
     1021 tattggctat cgcagcggcc aatcggtggg acggttgtcg tcccaatcgc ccggtcccga
     1081 tgcgcgcaag ctggtcgacg ccgcggagtt gggcattccc ttctcgatga atttgctggc
     1141 ggtgagcatc gatttctatc gcaagtcgcc aaaaacggtg gagggaatca tcgccgccta
     1201 tatcgaaggc atcgccgcgt tgaagcagcg caggcagcag gcctccaacg ttttggcaaa
     1261 atatatgggc cagcgcggcg gctcggccga cgttcactac gaattcgtca tgaaatatct
     1321 ggaagccgtg ccgcgagtcg agccggcggc catcgacacc gtgttggaaa tggtcggcag
     1381 caaagacgcg gcgaaggcca agctttacga caatagcgtc gtcgatcgtt tggtccaaga
     1441 agggttggtc gaccgactct acaaaggggc gaaaccatga ccgaaaaaca gtggcgcgag
     1501 tgcttgggcg agctcgtgcg cgattatatg cgctcgccgg agatggactt ttatttttcc
     1561 atcaagatga acaaacccag agccgcggtc atggtgcagc agcagagtct cttcgtgcgc
     1621 caccggcgcg actgttgggc ccatgtgtcg gccaattgcc cagtgatggc ggttaagcag
     1681 cgtattttgg agcatgagta cgaggagatc attcgcgacg attattccga ttatggccat
     1741 ctgcacttga tcatccgcca gggcaaatcg gtgggtctgg aacctgaggc gattatcaac
     1801 gctgagccga cgccggaaac ccgcgccgcg ctttatgcct gggcgtggat gacctgcagg
     1861 atggcgtgga ccgcatcgct taccggcatg acgatcaccg agtggtgcaa cgacgaccgc
     1921 ttgctcggcg acctgggcgg cggacagtcg acgcgcatgg cgaaaaaatg gatggaagat
     1981 atgggattta cctggaagca gattcccaac atgcaggcgc acagccaggc cgacgagaag
     2041 catagcgaca tgtttctgcc atttcttgcc gagcacgcca ccggcgagaa agaagcgtcg
     2101 gcgcttcaag ccgccaagga ttcgctggcg ttcaacgcca tgtatcgcaa aggcatcgcg
     2161 ctggcgcagg agaagattgg gctgtagaaa attgtgcgtg atgcagtagt gaagcgtttt
     2221 atcatcttgc ggggtgtgac ccacgcgtgt tgacagatgg tagagccggc ataaactagg
     2281 cgcactaaca cgaaacaacc tccgaggccg ttgcgtgcca cctaagatac gggaactgat
     2341 cggggacctg cagaaagcgg ggtttgttga ccgtggtg
//
LOCUS       DASQKS010000171         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3870521, whole genome shotgun sequence.
ACCESSION   DASQKS010000171 DASQKS010000000
VERSION     DASQKS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3870521"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..447)
                     /locus_tag="VE170_04290"
     CDS             complement(52..447)
                     /locus_tag="VE170_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14683.1"
                     /translation="MLKFAAVLLLLAMTAGCAEHFDHDTTLATKRAVEFAEATFVRGN
                     IDQGYAMLADKARAYVPLNIFADKVKQMHPSGRPTKIAGLSTAPVKGEKLVNVTLRGE
                     GDGQFMYGIVVIGTSAADYRVSTFSGGRS"
     gene            complement(533..2140)
                     /locus_tag="VE170_04295"
     CDS             complement(533..2140)
                     /locus_tag="VE170_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ14684.1"
                     /translation="MAAFNFKPTAFDSIDARATPLPSLPRRRESGFPSGWNLARSNDV
                     FRCAATLALFIASSCATVGPLAVAQSRQTTVELTPCTFARHKEAALCGKHQVYENREA
                     KTGRKIALNIVVLPARSSAAKTDPVFYLAGGPGQAAARIASAGEDAIMRELRRERDLV
                     FVDMRGTGDSNGLQCDFPADPSKVQSFFGEFFELAVIQACREKLERIADLKYYNTPLG
                     VDDLEEVRLALGYDKINLYGVSHGSQATLEYLRRYPDAVRSAVLAGVATPAAKLPLQF
                     AQGAEQAMTRLFEDCAADDGCNTAFPQLMQRFAQLMQSFDNGPVELQVAHPATKAVQT
                     ATLSRGVFVVRLLALLYNHRTAGLLPLIIANAAQGDWSAFMKILARSAAPPAFRVYLG
                     AYLSATCSESVPLIDDQALVRATAHTFMGEYRTNRHQQACAHWPRGEIAPHYYQPVRA
                     ATAVLMLSGDIDPATPAAFGAQALETLPNGRQVVLRNTPHSYESSCARALILTFIASG
                     SATELDTRCAARLRRPPFATELPASYK"
     gene            complement(2151..2984)
                     /locus_tag="VE170_04300"
     CDS             complement(2151..2984)
                     /locus_tag="VE170_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14685.1"
                     /translation="MTIIDAQVHIWQADTPERPHIKVDASKPHREIPLTYERLLAEMN
                     RAGVDRVILVPPSWDGYRNDYALTAAQKYPDRFAVMGKVPLNVPASKDQLPTWLKQSG
                     MKGFRISFRHSGTHSLLDDGSADWFWADCERYDIPVMIFAPFAVAAIGKIARRHPGLR
                     VIVDHMGLNVQWRGKDLTPGVDVLLTFAGLANVAVKASCLPCYVAENYPYPTLHPQLR
                     RVVDAFGPQRIMWGTDLSQSPISYRQNVTLFTEELDFLSTDDKEWIMGKSLAHWLGWP
                     I"
     gene            complement(3050..4048)
                     /locus_tag="VE170_04305"
     CDS             complement(3050..4048)
                     /locus_tag="VE170_04305"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14686.1"
                     /translation="MGFVINDAAIEKITRAVIAGIVLSIVMPFTGWAQSARPARPLRV
                     AYLSTSSTMASVWMAKDSGAFTKEGLDVEVLSMQSTSAIPALLANEIDVVQVSAAPVL
                     TAALRGIDVTFIAGLLNTMIWDFYARPEIKTIEQLKGKIVGTDRPGTPVAYGTMVALK
                     KMGLTTKDVQLRPLGSSPQIVAAFYAGQIAGGVGSPPASFRIERDGFHSLISLIGEPY
                     QNVGLVVRKSRIDELSARLVPLLRAVRSGIMRFYADKPFSMKVIGKYAYETDPDVLER
                     SYEFFKKAGFRRELVTSEPGLQGVLDFLSESIPEAKTAKPGQFFDDRILRQVNVTK"
     gene            4418..4579
                     /locus_tag="VE170_04310"
     CDS             4418..4579
                     /locus_tag="VE170_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14687.1"
                     /translation="MKNSNDSLFGFALAVVALVALLTVSHSWERSVSPLGGTYSKVSE
                     LGLRVSMHR"
     gene            complement(4608..>5267)
                     /locus_tag="VE170_04315"
     CDS             complement(4608..>5267)
                     /locus_tag="VE170_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14688.1"
                     /translation="LTGMFMAQPKIKSPAELRGKSVGVNSLSGGGWIFSMLTLDHWGL
                     SPERDKIQFRSLGDQTVMVQGFMAGTVDAVLVGYAHGKNLQNKGYTVLADVEKLPIPY
                     QGSGLMSRRQFMRTQPAVAENVFRGLLDAVNFIRNPANKTEVIRAVAKGMRLAKLDDA
                     EEGYKTLPTLYDQKIYPSVDGIRNVIRLLGLTNEKIRKLKAEELIEDSVLKKLEKEGR
                     F"
BASE COUNT         1085 a         1466 c         1606 g         1110 t
ORIGIN      
        1 ttcgcgacga gcagcagcgc cgccaggcgc aggctttcgg ccgttagcga cttaactgcg
       61 cccgccgcta aaagtgctga cgcggtaatc ggccgccgac gtgcctatca ccacgatgcc
      121 atacatgaat tgtccgtcgc cttcgccgcg caaggtgaca ttgacgagct tctcgccttt
      181 caccggggcg gtgctgagtc cggcgatctt ggtcggacgg ccggatgggt gcatctgttt
      241 gaccttgtcg gcgaatatgt tcaagggcac ataggcgcgc gctttgtcgg cgagcatggc
      301 gtagccttga tcgatattgc ccctgacaaa cgtggcttcg gcaaactcaa cggcgcgctt
      361 ggtggcgagc gtggtatcat gatcgaaatg ctcggcgcag ccggcggtca tggccagtaa
      421 caatagcacc gcggcaaatt tcaacatgat cgtttctccg gttcgttgcg gacaacgcac
      481 ttttctcacg agcggatggc gactagcgcc accctcgatc gccaataact cattacttat
      541 aactcgccgg cagctcggtt gcgaacggcg ggcggcgcaa acgcgccgcg cagcgggtat
      601 ccagctctgt ggccgaaccg ctggcgataa aggtcaggat tagggcgcgg gcacaggatg
      661 attcgtagct atgcggggtg ttgcgcagga ccacttggcg gccattgggc agggtctcga
      721 gcgcttgcgc gccgaacgcc gctggggtcg ccggatcgat atcgccggaa agcatgagca
      781 cggcggtcgc agctcgcacc ggttggtaat agtgcggggc gatctcgccc cgcggccagt
      841 gcgcgcacgc ctgttgatgc cggttggtgc gatattcgcc catgaaggtg tgggccgtgg
      901 cgcgcaccaa cgcctgatca tcaatgaggg gaacggactc cgagcaggtg gcgctcaaat
      961 aggcgccgag ataaacccgg aacgccggcg gtgcggcgga gcgggcgaga atcttcatga
     1021 aggcactcca atctccttgg gcagcgtttg cgattattaa gggtaacaga ccggcggtgc
     1081 gatgattgta gagcaaggcc aggagccgaa cgacgaacac gccgcgcgat aacgtcgccg
     1141 tttgcaccgc tttggttgcc ggatgagcca cttggagctc caccgggcca ttgtcaaagg
     1201 attgcataag ctgggcgaac cgttgcatca attggggaaa ggcggtgttg catccgtcat
     1261 cggcggcgca atcttcaaac agccgggtca ttgcctgctc ggcgccctgg gcaaattgca
     1321 atggcagctt ggcggcggga gtggcaactc ctgctaggac cgcactccgc accgcatcgg
     1381 gatagcggcg caggtattcg agagtcgcct gggatccatg cgagacgccg tacaaattga
     1441 tcttgtcgta acctaaggcg agccggactt cctcaagatc gtcgaccccc agcggagtat
     1501 tgtagtattt caggtcggcg attcgttcga gcttttcgcg gcacgcttgg atgaccgcga
     1561 gctcgaaaaa ttcgccaaag aaactttgca ccttgctggg atcggccggg aaatcgcatt
     1621 gcagcccatt ggaatcgccg gtgccgcgca tgtcgacgaa aaccaggtcg cgctcacggc
     1681 gcaattcgcg catgatcgca tcttcaccgg cgctggcgat gcgcgccgcg gcctggcccg
     1741 ggccgccggc caaatagaaa accgggtcgg tcttggctgc ggagcttcgc gccggcagga
     1801 caacaatatt gagcgcaatc ttgcggccgg tttttgcctc acggttttcg taaacttgat
     1861 gtttaccgca aagcgcggct tctttgtgtc tggcaaaagt gcagggagta agttcaacgg
     1921 tggtttgtcg actctgagcg accgccagag gtccgacggt tgcgcacgac gatgcgataa
     1981 aaagggcaag ggtcgctgca caacggaaaa catcattgga gcgcgctaga ttccaaccgc
     2041 taggaaaccc ggattcccgc ctgcgcggta atgacggaag gggagtcgcc cgcgcatcga
     2101 tggagtcaaa agccgtcggc ttgaaattga atgcggccaa gctcgccttg ctagatgggc
     2161 cagcccagcc agtgcgccag gcttttgccc atgatccact ctttgtcgtc ggttgagaga
     2221 aagtccaact cttcggtgaa cagcgtgaca ttttgccgat aggaaatcgg cgattgcgac
     2281 aagtccgtgc cccacatgat ccgttgggga ccgaaagcat cgactactct acgcaactgg
     2341 ggatggagcg tgggataggg ataattttca gcgacgtaac agggcaggca ggaagctttg
     2401 accgcgacat tggccaggcc tgcgaaggtt agcaatacat cgactccggg ggtgaggtct
     2461 ttgccgcgcc actgcacgtt caaacccatg tgatcgacga tcacgcgcag tccggggtgg
     2521 cgccgggcga tcttgccgat ggccgccacc gcgaagggtg cgaaaatcat caccggtatg
     2581 tcgtagcgtt cgcagtcggc ccagaaccaa tcggcgctac cgtcgtcgag aagcgaatgg
     2641 gtgccgctgt ggcgaaagct gatgcgaaaa cctttcatac cggattgttt gagccaagtt
     2701 ggcagctggt ctttgctcgc tggaacgttg agcggcacct tgcccataac ggcgaaccgg
     2761 tcgggatact tttgcgccgc tgtgagcgcg taatcgttgc gatagccgtc ccaggacggc
     2821 ggcaccagga tgacccggtc gacgccggcg cgattcattt ccgccagcaa gcgctcgtag
     2881 gtcagcggga tttctcgatg cggtttggaa gcgtcgactt tgatatgcgg ccgctccggc
     2941 gtgtcggctt gccatatatg gacttgggca tcgatgatgg tcatgggcag gcaaccggca
     3001 acaggcggcg gacagcagca agaaaaagtg aattcatttg caagctaagt tattttgtca
     3061 cgttgacctg gcgcagaata cggtcgtcga agaattgccc cggcttcgcg gtcttggcct
     3121 cgggaatgga ttcggagagg aaatccaaaa cgccttgcag ccctggttca gaggtgacca
     3181 gctcgcggcg aaagccggct ttcttaaaaa actcgtagct gcgctcaaga acgtctggat
     3241 cggtttcgta ggcgtatttg ccgatgacct tcatggagaa cggcttatcg gcgtagaatc
     3301 gcatgatgcc gctgcggacg gcgcggagca gcggcacgag cctggcactg agctcatcga
     3361 tgcggctctt tcgcactacc agtccgacat tttgataggg ctcgccgatc aggctgatca
     3421 gagaatgaaa gccgtcgcgc tctatcctaa aacttgccgg cggcgagccg acgccgccgg
     3481 cgatctgtcc ggcgtaaaat gccgcgacga tttgcggact gctgccgagc gggcgcagct
     3541 gcacgtcttt cgtcgtgaga cccatctttt tcaatgcgac catcgtccca taggcgaccg
     3601 gcgttcccgg ccggtcggta ccgactattt tacctttcaa ttgttcgatg gttttgattt
     3661 ccggacgagc atagaaatcc cagatcatcg tgttcaatag accggcgatg aaagtcacgt
     3721 cgatgccacg cagtgcggcg gtgaggacgg gggctgccga gacttggacg acatcgatct
     3781 cgttggcgag caatgccgga atcgcgctgg tcgattgcat ggagagcact tcgacgtcca
     3841 acccttcctt ggtaaaagct cccgagtcct tggccatcca caccgatgcc atcgtagacg
     3901 acgtgctcaa ataggctacc cgcagcggcc gagccgggcg tgcggactgc gcccagccag
     3961 taaacggcat gacgatactc agaactatcc cggcgatcac ggcacgggtg attttctcga
     4021 tggcggcgtc gtttatgaca aagcccatgg gattcgcctc caagcgcggc agtaaattgg
     4081 ttgagcgtaa gatagggcag gacgaagggt agcgtcaagg taaaatagcg agctccccaa
     4141 tcacagctgc actaggccag agtgtcagaa tagcgcaggc ccttccttca acctgtgagt
     4201 aggcgtccgg cggtttaaat ttttaaatgc acagtattgg ccgcgtgtag gtgcaaaagc
     4261 tcaggataat taccaaaatc tacgccgatg caccaagccc ccttgaggcc caagggttgc
     4321 gctgaatcca cgtgagtgtt agagcgaatt ggtgcgtaaa ctttttgccg ctgctcccgt
     4381 ctatctaggg tgttcagatc ggaaaggatg gtggcccatg aagaattcta acgattcact
     4441 ttttggtttc gccctggcgg ttgtggctct ggtcgcacta ctcacggtca gtcactcttg
     4501 ggagcgttcg gtgtcgccgt tgggcggcac gtattcgaaa gtcagcgaac ttgggttgcg
     4561 ggtttcaatg catcgctaac cggctcagct ggccggttca gcgatgctta gaaccgtccc
     4621 tctttctcaa gtttttttaa tacgctgtct tcgattaatt cttccgcctt caacttgcga
     4681 atcttctcat tggtcaagcc gagcaaacgg atgacgttgc gaatcccgtc gacgctcgga
     4741 tagattttct ggtcgtacaa agtcggcaag gtcttgtagc cctcctcagc atcgtccaat
     4801 ttcgccaagc gcatgccttt ggcgacggcg cgaatcacct cggttttgtt ggccgggttg
     4861 cggataaagt tgaccgcgtc gagcaagccg cgaaagacgt tttccgccac cgccggctgc
     4921 gtgcgcataa attggcgccg cgacattagt cctgagccct gatagggaat cggcagtttt
     4981 tccacgtcgg cgagcaccgt gtagcctttg ttctgcaagt ttttcccatg ggcgtagccg
     5041 accagcacgg cgtcgacggt gccggccata aaaccttgga ccatgacggt ctggtcgccg
     5101 agggagcgga actggatctt gtcgcgctcc ggcgacagac cccaatggtc caacgtcagc
     5161 atggaaaaaa tccaaccgcc gccgctaaga ctattgacgc cgacgctctt gcctcttaat
     5221 tccgccgggc tcttgatctt cggctgggcc atgaacatgc cggtaag
//
LOCUS       DASQKS010000172         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_510878, whole genome shotgun sequence.
ACCESSION   DASQKS010000172 DASQKS010000000
VERSION     DASQKS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510878"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VE170_04320"
     CDS             <1..405
                     /locus_tag="VE170_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HYJ14689.1"
                     /translation="RSASPTEDAGPRGFREVSVFELLAALKRIIDRLPKDSFHEVTLE
                     KITVREKMTHLLDILRAQPSILFESLFAEASSRMEIIVTFLAMLELVKVRAIRIVQEE
                     MAGPIVIEAAADMDGVAESVVLDQAEEDPYGA"
     gene            395..970
                     /gene="scpB"
                     /locus_tag="VE170_04325"
     CDS             395..970
                     /gene="scpB"
                     /locus_tag="VE170_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYJ14690.1"
                     /translation="MEREEVKSILESLLFVADGPLTVQRFAEVVDGVDKAMIHGLLQE
                     LQSDLEAPSRGLQLVEVAGGYQLRTAKINGDWVKKLLGGRPARMGRATLETLAIIAYR
                     QPITKAEIEAIRGVDVDGVITTLLERNLIRAVARKDVPGRPFLYGTTTEFLQLFNLKD
                     LTDLPTLKETDEISLPEISRDVIPLEQAEES"
     gene            983..1849
                     /locus_tag="VE170_04330"
     CDS             983..1849
                     /locus_tag="VE170_04330"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYJ14691.1"
                     /translation="MERIQKIIARAGLASRREAERWIVEGRVTVNGTVIDKLGSQADP
                     FKDSIKIDGKRVKSAAEPLYYALHKPPGVITTLNDPQHRPDITPYVARLGEKKRLFPV
                     GRLDYNTTGLLLLTNDGDLAMRIAHPRYGIPKLYQVKLSACPTEDDFAILRKGIRLED
                     GMTAPARVRVIEKLRKNAWVEIEVHEGKKREVRRMFEALNYFVEKLIRVKVGPIELGS
                     LPIGELRPLYQSEVMALKIAVGLAKPGPTVSGRRPVIPAKKSGGFNSRRSTYSNKPPS
                     PSGARNRTPRSR"
     gene            complement(1812..>2894)
                     /gene="moeB"
                     /locus_tag="VE170_04335"
     CDS             complement(1812..>2894)
                     /gene="moeB"
                     /locus_tag="VE170_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HYJ14692.1"
                     /translation="NNGKSPVLLDVRESDEWRQGHLEGAIPLPRGFLEIKVETAIPDK
                     NTPIVAYCAGGVRSLLAAKVMREMGYQNVTSMAGGYGAWKNGGFKWVQDFQYTPEQLI
                     RYSRHFLLPEVGEDGQAKLLQAKVFMVGAGGLGSPAAYYLAAAGVGTIGIIDNDVVDI
                     SNLQRQILHANDRVGMPKVESAKKTLEGLNPDVKVVPYQTKLTSENIMEILKDYDMVI
                     DGCDNFPTRYLVNDACVLAGKPNVHGSIFQFEGQATVFYPGKGPCYRCLYPEPPPAEM
                     APSCAEAGVLGVLPGLIGCIEALEAVKLILGKGDSLVGRLLHFNTLTMEINTLKLRRD
                     PNCPMCGDNPTIKELIDYEEFCSLRH"
BASE COUNT          621 a          805 c          871 g          597 t
ORIGIN      
        1 cgttcggctt cacccactga agatgccgga ccgcgcggct ttcgcgaggt gtcggtattc
       61 gagctgctag cggcgttgaa gcgcattatc gaccgcctgc ccaaagatag ttttcatgag
      121 gttacgttag aaaagatcac ggtgcgcgag aagatgaccc atctgctcga tatcctgcgg
      181 gcgcagccct cgatcttgtt cgaatctctg ttcgctgagg ccagttcgcg catggagatc
      241 atcgtgacct ttctcgccat gttggagttg gtcaaagtcc gggcgattcg tatcgttcag
      301 gaagaaatgg ccgggccgat cgtgattgag gccgccgccg acatggacgg cgtcgctgag
      361 agcgtagtgc tcgatcaggc tgaggaagat ccctatggag cgtgaggaag tcaaatcgat
      421 tttagaaagt ttgctgttcg tcgccgacgg gccgctgacc gtgcagcgat tcgccgaagt
      481 ggtcgatggc gtcgataaag ccatgattca tgggctgctg caggagctgc aaagcgatct
      541 cgaggcgccg agccgcggtt tgcaattggt ggaagtggcc ggcggctatc aactgcgcac
      601 cgccaagatc aatggcgatt gggtaaaaaa acttcttggc ggccggccgg cgcgcatggg
      661 ccgggcgacc ttggagacct tggcgattat cgcttatcgc cagccgatca ccaaagcgga
      721 gatcgaggcg attcgcggcg tcgatgtcga cggcgtgatt acaacgctct tggagcggaa
      781 tttgattcgt gcagtggcgc gcaaagacgt gccgggacgg ccgtttttgt atggcacgac
      841 caccgaattc ttacagttgt tcaatctcaa agacctgacc gatctgccga ccctcaaaga
      901 gaccgacgaa atttccctgc cggagatatc ccgcgacgtg atcccgctcg aacaggccga
      961 agaaagttag cgcacacccg agatggaacg gatacaaaag attatcgccc gggcgggcct
     1021 ggcttcgcgc cgggaggcgg agcgctggat cgtcgagggg cgggttacgg tcaatggcac
     1081 ggtgatcgac aagttgggct cccaagccga tccgttcaaa gacagtatca agatcgatgg
     1141 caagcgcgtg aagtccgccg ccgaaccgct ttattacgcg ctgcacaaac cgcctggtgt
     1201 gattaccacc ttgaacgatc cgcagcatcg tccagatatt acgccgtatg tcgcacgcct
     1261 gggggaaaaa aaacggctct ttccggtggg ccgcttggac tataacacca cgggtttgtt
     1321 gctcctgacc aacgacggcg atctggccat gcggatcgct catcccaggt atggtattcc
     1381 aaagctctat caggtgaagt tgagcgcttg tccgaccgag gacgattttg cgattttacg
     1441 caaaggtatc cggttggaag acggcatgac cgcgccggcg cgggtgcggg tgatcgaaaa
     1501 gctgcgtaaa aatgcttggg tcgaaatcga agtccacgag ggcaaaaagc gcgaggtgcg
     1561 ccggatgttc gaagcgctca actatttcgt cgagaagctg attcgcgtta aggtcgggcc
     1621 gattgaattg ggatctttgc cgatcggtga actgcggccg ttgtaccaaa gcgaagtcat
     1681 ggctctcaag atcgccgttg ggctggccaa gccagggccg acggtaagcg ggcgccgccc
     1741 ggtgatcccg gcaaaaaaaa gcggcggctt caacagccgc cgctccactt attcgaataa
     1801 accgccgtcg cctagtggcg caaggaacag aactcctcgt agtcgataag ctctttgatc
     1861 gtcgggttgt cgccgcacat cggacagttg ggatcgcggc gcagcttaag cgtgttgatc
     1921 tccatggtca gggtattgaa atgcagcagc cggccgacca agctgtcgcc cttgccgaga
     1981 atcagcttca ccgcttccaa cgcttcgata cagccgatga gtccgggaag aacgcccaag
     2041 acgccggctt cagcgcagct cggcgccatt tccgccggcg gcggctcggg atacaaacag
     2101 cgatagcagg ggcctttgcc cggatagaaa accgtcgcct gtccctcgaa ctggaaaatg
     2161 ctgccatgga cgttgggctt gccggcgagc acgcaagcgt cgttgacgag gtagcgcgtg
     2221 ggaaagttgt cgcagccgtc gatcaccatg tcgtagtcct tcaagatctc catgatgttc
     2281 tctgacgtga gcttggtctg atagggcacc accttcacat cgggatttaa cccttccaaa
     2341 gttttctttg ccgactcgac cttgggcatg ccgacgcggt cgttggcgtg caaaatttgc
     2401 cgctgcaagt tggaaatatc gacgacgtcg ttgtcgatga tgccgatagt gccaacaccg
     2461 gccgccgcca gatagtaggc tgcgggcgag cccagaccgc cggcgccgac catgaatact
     2521 tttgcttgga gcaatttcgc ctgaccgtct tcgccgactt ccggcagcag aaaatggcgg
     2581 ctgtaacgga tcagctgctc cggcgtgtac tgaaaatctt gcacccactt gaagccgccg
     2641 ttcttccagg cgccgtaacc gccagccatg gaagtgacgt tctgataacc catctcccgc
     2701 atcactttgg cagccagaag cgaccgcacg ccgccggcgc aataggcgac gatcggcgta
     2761 ttcttgtcgg gaatagcggt ctcgactttg atttcgagaa agccacgggg cagcggaatg
     2821 gctccttcca aatgtccctg gcgccactca tcgctctcgc gaacatcgag taaaaccggc
     2881 gacttgccgt tatt
//
LOCUS       DASQKS010000173         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5130309, whole genome shotgun sequence.
ACCESSION   DASQKS010000173 DASQKS010000000
VERSION     DASQKS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5130309"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1256)
                     /locus_tag="VE170_04340"
     CDS             complement(258..1256)
                     /locus_tag="VE170_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14693.1"
                     /translation="MMKQLGCLGHLVLLAMLLGAGNLHGAEKIRVSGGGITPLHSLIW
                     VAHQEGLFKKYGMDVEYLVMNSGTLGVQTLLSNDSQFLFSTGALAVTANLQGADLTMI
                     TGGFNLFAFKVVGRPEIKSVQDLRGKKVSISQFGSATDFAVQASLEKFGVNPKQVTVI
                     QLVSSSNRLTGLTNGATEASLFTEPFATMAIKKHRMALLLDMAEAGMAYPQSCLMIKR
                     SYLEANKERATNFVKALVEALFIAKRDRALTIQVIKKYIRADDEVYGIGYDYFLGTHA
                     EGLLSLPERKGVELVIAQLAKTNPKAKGQTVESLKVMEPSILDELRRSGFIDKVRR"
     gene            complement(1342..1782)
                     /locus_tag="VE170_04345"
     CDS             complement(1342..1782)
                     /locus_tag="VE170_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14694.1"
                     /translation="MDKLKLDAVVHWSIPVNDLAAAEKFYGEILGLEHVGRLANTPMS
                     CFKLGNHNILLCQRRDPINRTIEQDGRLHHAFDVSAEMFDKACKLFHRLAIKIHEPVD
                     YRPGGFFTGRQLFVLDPSGNRIELRDASWKQGMPTPTYEEIVAS"
     gene            1909..2874
                     /locus_tag="VE170_04350"
     CDS             1909..2874
                     /locus_tag="VE170_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14695.1"
                     /translation="MANRIITIGMRDYDHCRALANGRVKVDGADLKFVNISPPSQIFL
                     RMLHDEEFDASEMSLSNFMIAMGKDDRRFVAIPVFPSRVFRHSYIWINTKAGIVKPAD
                     LKGKKVGIADYSMTALLFVRGLLQHEYGVTPGDIHWFRRRAEHVAVEIPPGIRLDNIG
                     KDQTLDGLLEAGELDAVAVTSPPRAFLQGSPLVARLFSDCRAVEADYYRRTKIYPIMH
                     MTVMRRAIYEQDPTLAVRLSQGFQAAKELAFEDYDEGLASLPWVNLDMEFARRVLGAD
                     VAPYGVKHNAATLEAATLYSHEQGLTKRKFAVDELFAKETLDLFS"
     gene            complement(2892..3908)
                     /locus_tag="VE170_04355"
     CDS             complement(2892..3908)
                     /locus_tag="VE170_04355"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14696.1"
                     /translation="MRMLHELGLHYRLALIVFMLGASPWAEAQERFRVSYGGFNETAT
                     SMWVGIERGMFKKYGIDAQMVQVRNGALSIATLVAKEVEAVWPAQSTILSTVSGGIKL
                     TCIGGPVNKIPRNLMVRKEIKGAEDLRGKTVGVQSIGGGLWLQTMIILDHLGVDPDKH
                     GIKVRVIGDEATLAQAMITNNIDFGVITYGLSETLYPQGFKSLIDAADISAPYQGPEI
                     CGLKESVASRNEFYSRVIKGLAEAVAYILDDNNKSDVAKVVQRNLRLSRPDVIEGSYK
                     VLRKMTTLDLAPNVAAFKSVQRIVARINPKIVQVDLDQVIDASFVRQLESSGFLAELR
                     KKIK"
     gene            complement(3966..4718)
                     /locus_tag="VE170_04360"
     CDS             complement(3966..4718)
                     /locus_tag="VE170_04360"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14697.1"
                     /translation="MRYGWRARIGHIAPAILDTSGEEMRKLLPAGVLHVGLTISEPIQ
                     TLGAEQAAEAFGRMVDAGKRLAAEKVDVLICGGAPVALSKGVAGDAELTELLRRETQL
                     PVVTANGAVIDGLRNLGARSVLVVSPFIDARNQEIKAFLEANKFAVPATRGLGLTKNI
                     DFASQSPDAPFTLARDLAKQHPEADAIYIACPRWPSVDIIAALEADTGKSVVAAPAAM
                     VWGALKALRINDCRVGFGRLMESLRSANQRPG"
     gene            complement(4931..>5449)
                     /gene="pal"
                     /locus_tag="VE170_04365"
     CDS             complement(4931..>5449)
                     /gene="pal"
                     /locus_tag="VE170_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HYJ14698.1"
                     /translation="AVIFLVSCGNPANPPPPKWTAENPSTESAKPAAPKPPPVASKAE
                     EKTSLEALQKGEATTSGPLKDVGFNFDSAALSEAARATLKSNADWLKANAATRIQIEG
                     HCDERGAADYNMALGAKRAQAALDYLATLGIAANRMSTISYGEEIPLCKEQSEACWAQ
                     NRRARFVVSAAK"
BASE COUNT         1118 a         1651 c         1459 g         1221 t
ORIGIN      
        1 tgatcgccgg cacagtagaa atgtgcgatc ccgcgtgatc aatcatgcgg atatcgcccg
       61 ggccacggtt catcgccgcg cacgccgaca acataccgat taaagatatg gctaaaatcg
      121 ccttaacagt ttcgaccatc acgtctccat cccatgcttc atgctttgta gaacgttcca
      181 caaaggcggg ttcaaacccg ccctacgatt gaggacatgt tttgcggctt tggtgttctt
      241 tgcggccaac tctccatcta tcttctgacc ttgtcgatga aaccgcttct cctcagctcg
      301 tcgagaatac tcggctccat cacttttaac gattccactg tctggccttt cgctttcgga
      361 ttggtttttg ccagctgggc gatcaccagc tcgacgcctt tgcgctccgg caggctcaaa
      421 agtccctcgg cgtgggtccc gagaaaatag tcgtaaccga tgccatacac ctcgtcgtcg
      481 gcgcgaatgt atttttttat cacctggatc gttagcgcgc gatcgcgttt ggcgataaac
      541 agcgcctcca ccaacgcctt gacgaagttg gtcgcccttt ccttgttggc ttcgagataa
      601 ctgcgtttga tcatcaagca gctctgcggg taagccatgc ctgcttccgc catgtccaag
      661 agcaatgcca ttcgatgttt cttgatcgcc atggtcgcga atggctcggt gaacagcgag
      721 gcttcggtgg caccgttggt cagccccgta agccggttgg aactgctgac cagctgaatc
      781 acagtcacct gtttggggtt gacgccgaac ttttccaaac tcgcctgcac cgcgaaatcg
      841 gtggctgaac cgaattggct gatggaaacc ttcttgccgc gcaagtcttg cacgctcttg
      901 atctccggcc ggccgaccac tttgaaagcg aacaggttga atccgccggt gatcatcgtg
      961 agatccgcgc cctgtaaatt ggcggtgacc gccagcgcgc cggtggaaaa gagaaactgg
     1021 ctgtcgtttg acagcagcgt ctgcacaccc aaagtgccgc tattcatcac cagatattcg
     1081 acatccatgc cgtatttttt gaacagccct tcctggtgag caacccaaat gagcgaatgg
     1141 agcggcgtta tgccgccgcc gctgacacgg atcttttccg cgccatgcag attgccggcg
     1201 cctaatagca ttgccaacag gactaagtga cctagacaac ccagttgctt catcatgccg
     1261 ccttgccgag cgaagcgatg gcgcgtccag aaagattcga acggcttgaa cgttttgaac
     1321 cgtttgagcg gacgcgcagc gtcacgacgc gacgatctcc tcatacgtcg gcgtcggcat
     1381 gccttgcttc caactggcgt cgcgcagttc aatgcgattg ccgctcggat cgagaacgaa
     1441 gagctgccgg ccggtgaaaa aaccgccggg ccggtaatcc accggctcgt gaattttgat
     1501 tgcgagccgg tgaaaaagct tgcaagcctt gtcgaacatc tcggcgctga cgtcaaaggc
     1561 gtggtgcagc cggccgtcct gttcaatcgt gcgattaatc ggatccctgc gctggcacag
     1621 aagaatgttg tgattgccga gcttgaagca ggacatcggt gtgttcgcca ggcggccgac
     1681 atgctccagg ccgagaatct cgccgtaaaa tttctccgcc gccgccaaat cgtttaccgg
     1741 aatcgaccag tgtacgacgg catcgagttt gagtttgtcc atagatcaac tccttccgac
     1801 tctccattat cccatccgct ccaacctgtc cactggacag agagagaatc gacgattgac
     1861 ttccgcagac cattcaactt acatttgcca tttcgaagcg gaggacatat ggcgaatcgc
     1921 atcatcacca tcggcatgcg cgactacgat cactgccgcg ccctggccaa cggccgcgtc
     1981 aaagtcgacg gcgccgatct caagttcgtc aacatctcgc cgccgtcgca aattttcctg
     2041 cgcatgttgc acgacgagga gttcgacgcg tcggaaatgt cgctatcgaa tttcatgatc
     2101 gccatgggca aggacgaccg ccgcttcgtc gctattccgg tgtttccatc ccgagtgttt
     2161 cgccattcct atatctggat caataccaag gcgggcatcg tcaagccggc cgatctcaaa
     2221 ggcaagaagg ttggcatcgc cgattactcg atgaccgcgc tcttgttcgt gcgcggcctc
     2281 ttgcagcatg aatacggcgt aaccccggga gacatccact ggtttcgccg ccgcgccgag
     2341 catgtcgccg tcgagatccc gccgggcatc cgcctcgaca acatcggcaa agatcagacc
     2401 ctcgacggcc tgctcgaagc tggcgagctc gatgccgtcg ccgtgacttc gccgccgcgt
     2461 gcctttcttc agggttcgcc tttggtggcg cggctgtttt ccgactgccg cgccgtcgaa
     2521 gcggactatt accggcggac gaaaatctat ccgatcatgc acatgacggt gatgcgccgg
     2581 gcgatttacg aacaagaccc gacgttggca gtgcgcctgt cgcaaggctt tcaagccgcc
     2641 aaggagctcg ccttcgagga ttacgacgag ggcctggcat cgctaccgtg ggtcaatttg
     2701 gatatggaat tcgcccgccg ggtactcggc gccgatgtcg ccccctacgg cgtcaagcac
     2761 aacgcggcga ctttagaagc ggcgacgctc tactcgcacg aacagggact gaccaagcgc
     2821 aaattcgccg tcgacgaatt gttcgccaag gagactttgg atttgttttc ctgagaagaa
     2881 ggccgcgccg gttatttgat cttcttgcgc agctccgcta gaaagccgct cgactccaat
     2941 tgacgaacga agcttgcgtc gatgacttga tcgagatcga cctgaacaat cttcgggttg
     3001 atacgcgcaa cgatgcgctg aacagattta aaagctgcga cgtttggcgc caggtcgagg
     3061 gtagtcatct tgcgcaacac cttgtaagag ccttcgatca cgtcggggcg gctcaaacgc
     3121 agatttcgct gcacgacttt ggcgacgtcc gacttgttat tgtcgtcgag gatgtaagca
     3181 accgcttcgg ccaacccttt gatgacgcgc gagtaaaatt cgttgcggct ggcgacggat
     3241 tccttcagcc cgcaaatttc cggcccctgg taaggagctg agatatcggc ggcgtcgatc
     3301 aatgatttga atccctgcgg gtataatgtc tcgctcaaac cgtaggtgat gacgccgaag
     3361 tcgatgttat tggtgatcat cgcctgggcc aaagtcgctt cgtcgccgat gacgcgcact
     3421 ttgatgccgt gtttatccgg atcgacgcca aggtgatcca agatgatcat cgtttgcagc
     3481 cacaaacccc cgccgatact ttgcacgccg acggttttgc cacgcaagtc ttccgcgccc
     3541 ttgatctctt tgcgaaccat gagattgcgc gggatcttat tcaccgggcc gccgatacag
     3601 gtaagcttga tgccaccgga tacggtgctc aaaatcgtcg actgcgccgg ccaaaccgct
     3661 tcgacctctt ttgcgaccag agtcgcgatg ctcagcgcgc cgttgcgcac ctgaaccatc
     3721 tgcgcgtcga tgccgtattt cttaaacatg ccgcgctcga tgccgaccca catcgacgtt
     3781 gcggtttcat tgaatccgcc gtaggaaacc cggaaccgct cctgcgcctc agcccatggg
     3841 cttgcgccga gcataaagac gatcaacgcc aatctgtaat gcagtcccaa ctcatgcagc
     3901 attctcattg aggcccccct gacgagacga atacactcgt tctatgcggc gtcgatttgc
     3961 ctggctcagc cgggtcgctg gttcgcgctg cgcagacttt ccatcaggcg tccgaagccg
     4021 actcgacagt cgttgatccg cagcgccttg agcgcacccc aaaccatcgc cgccggagcg
     4081 gcgacgacgc tcttgccggt gtccgcttcc aatgccgcga taatatccac cgaaggccag
     4141 cgcggacagg caatataaat cgcgtcggct tcgggatgct gtttggcgag atcgcgcgcc
     4201 agcgtaaatg gcgcatcggg cgactgactg gcgaagtcga tatttttcgt caaacccagt
     4261 cctcgggtcg ccggcactgc gaatttgttc gcttcgagaa aagctttgat ttcttgattg
     4321 cgcgcgtcga tgaacggact gacgacaagc accgagcgcg cgcccaagtt acgcaaaccg
     4381 tcgatcaccg cgccgttcgc cgtgaccacc ggcaactgag tctcgcggcg gagcagttcg
     4441 gtcaactcgg cgtcgccagc cacgccctta gacaacgcca cgggcgcgcc gccgcagata
     4501 agcacatcga ctttttccgc ggccaagcgc ttgccggcat cgaccatgcg tccgaacgcc
     4561 tcagcagcct gttccgcgcc gagagtttga atcggttcgc tgatcgtcag acctacatgc
     4621 agcactcctg caggcaacaa cttgcgcatt tcttcgcccg atgtgtcgag tatcgccggc
     4681 gcaatatgac cgattcgtgc tcgccaaccg taacgcatgg aattcacctt tttctggctc
     4741 ggctgagtta tcgctcacgc ccaggcagcg aagtgcgaac ctaacaaacg cccgctttct
     4801 atgtcaatcg atagccacga ttatggtcgg atcctgtgcg tcaattcaat cgccggctca
     4861 cgccgcagca aaaaaaaagg cccatgtcac catgacacgg gcctttctcg aattgatatt
     4921 cgaacggaag ttatttggct gcgctaacga caaatcgcgc gcgtcgattc tgcgcccagc
     4981 atgcttcact ctgttctttg cacagaggaa tttcttcacc gtagctgatc gtcgacatgc
     5041 gattggcggc gatgcctaaa gtcgccaggt aatccagcgc tgcctgggcg cgcttggcgc
     5101 ctagcgccat gttgtaatct gccgcgccac gttcgtcgca atgaccttcg atctgaatac
     5161 gcgtcgcagc attggccttg agccaatcag cgttgctttt taacgttgcc cgggcggctt
     5221 cagaaagcgc cgcgctgtcg aagttgaagc cgacatcttt aagcggtccg ctggtcgtgg
     5281 cttcgccctt ttgtagcgcc tctaaactcg tcttctcttc cgctttcgac gccacgggcg
     5341 ggggtttcgg cgccgccggc tttgctgact cagtcgaagg attctccgcc gtccatttcg
     5401 gcggcggcgg atttgcggga ttaccgcatg aaactaaaaa aataaccgc
//
LOCUS       DASQKS010000174         5931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2196546, whole genome shotgun sequence.
ACCESSION   DASQKS010000174 DASQKS010000000
VERSION     DASQKS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196546"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /gene="cysC"
                     /locus_tag="VE170_04370"
     CDS             <1..1319
                     /gene="cysC"
                     /locus_tag="VE170_04370"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HYJ14699.1"
                     /translation="EFTSITAIPLSALEGQNILEPAPAIMPWYHGQSLLHWLETVPSA
                     ERQSTQAFTMPVQWVNRPNLDFRGFSGRIGSGSIRPGDRVRVSPSGVVSAVKEISVWG
                     GPLPEADAGDSVTLVLENEIDASRGDVIAAADRPIEVADQFEAEILWMSEHPMLPGRT
                     YAGQIHSKSANVSITDIKHRLDINSGAHLAAKALGLNEIATVNVSFDRPVPYAAYRDN
                     HRLGAFILIDKLTHETVCAGVIHFALRRAVNVHWQALEVTKPARAGLKQQQPRCLWFT
                     GLSGSGKSTVANLLEKRLHADGWHTYILDGDNVRHGLNRDLGFTEADRVENIRRVAEV
                     AKLMVDAGLIVIVAFISPYRAERQLARGLFDSSEFVEIFVDTPLPECERRDVKGLYAK
                     ARRGELKNFTGIDSAYEAPEAPEIHLRTMEAQPATCVERIVEWLRS"
     gene            1722..2102
                     /locus_tag="VE170_04375"
     CDS             1722..2102
                     /locus_tag="VE170_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14700.1"
                     /translation="MAENDSGVLRRSLNDFLSIQLDETALNRLTWRNFGNGLEMSRLA
                     REGKRELVLYRIAADASPDAFVKHEHLSGEFYLVLKGKICDETGDYETGDVVFLDPRS
                     VHTPRAVGETVVLVLWPAGVRLVD"
     gene            2135..3013
                     /locus_tag="VE170_04380"
     CDS             2135..3013
                     /locus_tag="VE170_04380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14701.1"
                     /translation="MANLGFIGTGGMGSAMAANLIKAGNKLAITDLRREQCKGLEGLG
                     AQFKDSPKAVAESCDVVCSMLPYNQAVQAVGLGKGGLHEAASGAKLWIDFSSIDKKTI
                     VGVDAELNKKGWTILDGSAGGVEEAAAAGTLSLWLSGSKAVFDQHQDIFKAMGNKIIF
                     VGELGNAKLVKNAMAMFAAVMHLSLAETCAWLKKGGLSEDIFRTILKNSQQDSVAIDR
                     IMEIIVSKKYKPRKSWMPKDVGFGLDMAREMEVPMPFVALAHQMFAIAQATGQDGYEA
                     TGIACNVYDIITGAKK"
     gene            3024..3560
                     /locus_tag="VE170_04385"
     CDS             3024..3560
                     /locus_tag="VE170_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HYJ14702.1"
                     /translation="MAPATERLSRFLTFLLRHKPREYPLTMDREGFTPWHEVVDVVRQ
                     RFYDVTEEQMRALIAGGSKKRFEIRGGKVRATFGHSFAIDPGGTAAEPPSELYFAAAR
                     NLTQSMLERGLEPRERQYVHLSVTAAEAESVARRHDPAPAMLVIDAQRAHAEGVRFYQ
                     SGPLFLAENVPAKFLSLR"
     gene            3926..5608
                     /gene="argS"
                     /locus_tag="VE170_04390"
     CDS             3926..5608
                     /gene="argS"
                     /locus_tag="VE170_04390"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYJ14703.1"
                     /translation="MRSYLKAILTQAIEKTAMEGELNIAEIPPLHLEPPKQREFGDLS
                     SNVALLWAKSAKKAPRAIAEAILKNIEDRDGLLARQEIAGPGFLNFAFAPKFYLAQMR
                     EIAAGKQDNIDIGQGRRVQVEFASVNPTGPLHVGHGRVAVIGDVLARLHEVTGFTVER
                     EYYVNDAGNQMENLGLSLYARYLQHMGRPAEFPEGGYPGDYVKDIAAAVFARDGDKFL
                     HQDKHEAVAFFRQYGGAELLNTIRAQLSEFGIRFDAYFSEKAMREREEVATAMDLLRR
                     LGVLYTHDGAEWFRSTRFGDDKDRTVIKSDGELTYFAADIAYHRNKFERKFVKLINVW
                     GADHHGYVPRLKAAMQALGYDANALQVVLVQMVQLTRGGEPVRMGKRTGEFVALEDVL
                     QEVGRDAARFFFLMRKSDSHLDFDLDLAKKQSSENPVFYVQYAHARVASIFEQAAKSA
                     ITVGTLASVPVERLELPEELELIRQMILFNEVLEESVRELEPHRMVFYLLELAGEFHR
                     YYNRNRVVSEDVDLSRARLLLLANIQKTLRRGLEILGVDAPMKMAARVETGV"
     gene            5640..>5931
                     /locus_tag="VE170_04395"
     CDS             5640..>5931
                     /locus_tag="VE170_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14704.1"
                     /translation="MAENRRGKDSRYYLSRGQMIILGGTFTLASIVVFLIGMMVGKEL
                     EERKLIKKDEPLVKMPVKPAGGAAGTAAKNDEITFYDTLAKSRDAQALAEPPA"
BASE COUNT         1269 a         1603 c         1791 g         1268 t
ORIGIN      
        1 tagagttcac cagcatcacg gcgatcccgc tgtcggcgct tgaagggcag aatattcttg
       61 agccagcccc tgcgattatg ccttggtatc atggccagag tttgctgcat tggctggaaa
      121 cggtgcctag cgccgaacgc caatctactc aagcgttcac catgcccgtg caatgggtta
      181 atcgacccaa cctggatttt cgcggcttct ccggacgcat cggcagcggc tcgattagac
      241 cgggcgatcg cgtgcgggtc agtccgtcag gagtcgtcag cgcggtcaaa gaaatctcgg
      301 tttggggtgg ccccctgccg gaggccgatg ccggcgattc ggtgacgttg gtgctggaaa
      361 atgaaataga tgccagccgt ggcgatgtca ttgccgccgc cgaccggccg atcgaagtgg
      421 cggaccagtt cgaggcggag attctctgga tgagcgagca tccgatgttg ccggggcgca
      481 catacgccgg gcagatacac agcaagtccg ccaacgtgag catcaccgac atcaagcatc
      541 gtctcgatat caacagcggc gcccacctcg ctgccaaagc gctgggcttg aacgagatcg
      601 ccaccgtcaa tgtcagcttc gaccgtccgg tgccctatgc cgcttaccgt gacaaccatc
      661 gtttgggggc gttcatatta atcgacaagc tgacccatga gaccgtctgc gccggtgtga
      721 ttcattttgc cctacgccgc gccgtcaatg tgcattggca ggcgctcgaa gtaaccaaac
      781 cggcgcgcgc tggcttgaag cagcagcagc cgcgctgctt gtggtttacc ggcctgtcgg
      841 gctccggcaa atcgacggtc gccaacctat tggaaaaacg tctccatgcc gacggctggc
      901 acacctacat ccttgacggt gacaacgtgc gccacggtct taaccgcgat ttgggcttta
      961 ccgaggccga ccgagttgaa aacattcgcc gcgtcgccga ggtggcgaaa ttgatggtgg
     1021 atgccgggct catcgttatc gtcgccttca tttcgcccta ccgcgccgaa cggcagctgg
     1081 cgcgcggtct gttcgactcc agcgagttcg tcgaaatatt tgtcgacacg ccgttgccgg
     1141 agtgtgaacg tcgcgatgtc aaaggcctct acgccaaagc ccgccgcggc gaactgaaaa
     1201 atttcactgg tatcgatagc gcctatgaag cgccagaggc accggagata cacttgcgca
     1261 ccatggaggc gcaacccgcc acttgcgtcg agcggatcgt cgagtggctg cggtcgtgac
     1321 gcaagaagcc ttgcattagg atatatccca cctatttcca tagattcacc gatgcgaacg
     1381 gttggaacac ggcagtgccc ggctgcggct gaggcgaaag cgcaaatata aatatccccg
     1441 tggagccggt tagtatattc atactccagc cctggcttgc acggttcgta tgccgaatgg
     1501 tttagatatt ttctgtcatt gaggcgtgcc ggaaagtccg tcgccctcgc cgcaccagaa
     1561 ctatttgcaa attcacctga cgggccgcaa ccggcaatat cgaaagaaga tcgagagcca
     1621 ggaccggagg cgattccgcc tgttgggtgt ccaggatacc cgctcaagta atctcatcac
     1681 gccttctttt gttcgccaat tagccgtgat agttaaacgc cgtggcggag aatgactccg
     1741 gcgtattgcg ccgctcgctt aacgattttc tctcgattca gctcgacgaa acggcgctaa
     1801 accgtttgac ctggcgcaac tttggcaatg ggttggagat gtcgcgattg gcgcgggagg
     1861 gcaagcgtga actggtgctc tatcgcattg ctgccgatgc ttcgcccgat gcatttgtta
     1921 agcatgagca tctgagcggt gagttctatc tcgttctcaa gggcaagatt tgtgatgaaa
     1981 ccggcgacta cgagactggc gacgtggtgt ttctcgatcc tcggtcggtg cacacgccgc
     2041 gagcggtggg cgagaccgtg gtactggtat tgtggcccgc tggcgtgcgt ttagtcgatt
     2101 gatttaagtt gattaggatt ttccaggagg caacatggca aatttaggat tcatcggcac
     2161 cggtggcatg ggcagcgcca tggccgccaa cttaatcaag gccggcaaca agttagccat
     2221 taccgatctg cgccgcgagc aatgtaaagg tttggaaggc ctgggcgccc agttcaaaga
     2281 ctcgcccaag gcggtggccg aatcctgcga cgtggtgtgc tcgatgttgc cttacaacca
     2341 agcggtgcaa gccgtcggct tgggtaaagg tggactgcat gaagcagcca gcggagccaa
     2401 gctatggatc gattttagca gcatcgataa gaagaccatc gtcggtgtcg atgccgagct
     2461 taataaaaaa ggctggacaa ttctcgacgg ctcggcaggc ggcgtcgaag aggcggccgc
     2521 cgccggcact ttgtcgctct ggctgtcggg ctcgaaagcg gtgttcgacc agcatcagga
     2581 tatcttcaag gcgatgggca ataagatcat tttcgtcggt gagttgggca acgccaagct
     2641 ggtaaaaaac gccatggcga tgtttgccgc agtgatgcac ttgagtttgg ctgagacttg
     2701 cgcttggctg aaaaaaggcg gcctgagcga ggacatattt cgcacgatcc tgaaaaattc
     2761 ccagcaggat tccgttgcca tcgaccggat catggaaatc atcgtcagca agaagtacaa
     2821 gccgcgcaag tcgtggatgc ccaaagacgt cggtttcggt ctcgacatgg cacgcgagat
     2881 ggaagtgccg atgcctttcg tcgcattggc gcaccagatg tttgcgatcg cccaggccac
     2941 cggtcaggat ggttacgagg ccaccggcat cgcctgcaat gtctacgaca tcatcaccgg
     3001 ggcgaaaaaa tagtgagacc catatggcgc cggcaacgga acgattgtcg cgctttttga
     3061 cctttctcct acgtcataaa cccagggagt acccgttgac gatggaccgc gagggcttta
     3121 cgccgtggca tgaagttgtc gacgtggtgc ggcaacgctt ctacgatgtc accgaggaac
     3181 agatgcgcgc gcttatcgcc ggtgggtcga aaaagcgttt cgagattcgc ggcggcaagg
     3241 tacgagctac ctttggccat tcgtttgcca tcgatccggg tggcacggcg gcggaaccgc
     3301 cgtccgaact ttactttgcc gccgcccgca atttaacgca gtcgatgctt gagcgcggcc
     3361 tcgaaccgcg ggagcgtcag tatgtacact tgagcgtcac agcggcagag gctgagtcgg
     3421 tggcgcgacg ccatgatccg gcgccagcga tgctggtcat cgacgcccag agggctcacg
     3481 ccgagggcgt ccggttttac cagtccgggc cgctgttcct agccgagaat gtgcccgcga
     3541 agtttctgtc gctgcgttag tctaggccat ttgaccgatt ccccggccat acgcattcac
     3601 tctatggtac aaatctcccg cgatcatctt gccagtctgt tgaaacctcg cgccggtgga
     3661 tcggtaattg caacgtgatc gccctagtgc gcggacggtt tgctgtgccg ccgtgtttcc
     3721 ctccccgaaa ccaccggatg agtatcatgg gtctggaggt ggattgatag cgctgagtga
     3781 tggcgcttga ggctggtttg cacgcgacat ttcattcagt tacattgttt gatcgttagt
     3841 agttaatccc aaaaaatttc aatcgaatcg tctggtctgg ggcgaatttg ccgtccggta
     3901 tagcggcgga tacataagca gcgcgatgcg aagttatcta aaagcaattt taactcaagc
     3961 tatcgagaag acagcaatgg agggagagct caatatcgcg gagatcccgc cgctgcatct
     4021 tgaaccgcca aagcagcgcg agtttggcga tctatcgtcc aacgtcgccc tgctctgggc
     4081 caagagcgct aaaaaagcac cgcgagcgat cgccgaagcg atcttaaaaa atatcgagga
     4141 tcgggacggt ctgctggcgc gccaagagat cgccggaccg ggatttttga attttgcctt
     4201 cgcgccgaag ttttacttgg cgcaaatgcg cgagatcgcc gccggcaagc aggacaacat
     4261 cgatattggc caggggcgca gagtgcaagt ggagttcgcc agcgtcaatc caaccgggcc
     4321 gctccatgtc ggtcatggcc gggtggcagt gatcggcgac gtcttggcgc gcttgcacga
     4381 agtcaccggg ttcaccgttg agcgtgagta ttacgtcaac gacgccggca accagatgga
     4441 gaaccttgga ctgtcgcttt acgcgcgcta tctccaacac atgggccggc cggcggagtt
     4501 cccggagggc ggttatcctg gcgactacgt gaaggacatt gccgctgcag tgttcgctcg
     4561 cgatggcgac aaatttctgc accaggacaa gcatgaagcg gttgcgtttt tccgtcagta
     4621 tggcggagca gagctgctca acaccatccg cgcacagcta agtgagttcg gcattcggtt
     4681 cgatgcttat ttcagcgaaa aagcgatgcg cgagcgcgaa gaagtcgcca cggccatgga
     4741 cttgctgcgc cggctcggcg tgctgtacac ccacgatggc gcggagtggt ttcgctcgac
     4801 ccgtttcggc gacgacaaag atcgtacggt aatcaagagc gacggcgagc tgacttactt
     4861 cgctgccgac atcgcctatc atcgcaacaa gttcgagcgc aagtttgtca agcttatcaa
     4921 tgtctggggc gccgatcatc atggctatgt gccgcgtttg aaggcggcga tgcaggcttt
     4981 ggggtacgat gccaacgctt tacaggtggt gctggttcag atggttcagc tgacccgcgg
     5041 cggcgaaccg gtgcgtatgg gtaagcgcac cggcgaattc gtcgcccttg aagacgtgct
     5101 ccaagaagtg gggcgcgacg cggcacggtt ttttttcctt atgcgcaaat cggatagcca
     5161 cctcgacttc gatttggatt tggcgaagaa acaatcgagt gagaacccgg tgttctacgt
     5221 gcaatatgcc cacgcgcggg tggcgagtat ttttgaacaa gcggcaaaaa gcgcgatcac
     5281 tgtgggcacg ttggcatcgg tgccggtgga gcgcttagag ttgccagaag aattggaact
     5341 gatccgccag atgatcctgt tcaatgaggt tttggaggag tccgtgcgcg agctcgaacc
     5401 tcaccgcatg gtattttatt tgttagagct ggccggggag tttcatcgtt actacaaccg
     5461 caaccgtgtg gtgtccgaag atgttgactt gagccgggcg cgtttgctgc ttttggcgaa
     5521 tattcaaaag acgcttcgcc gcgggttgga gattctcggg gtcgatgcgc cgatgaaaat
     5581 ggccgcaagg gtggaaaccg gagtttgacc ggcccgcgaa gggcaagggg ttaatcgaaa
     5641 tggcggagaa ccgtagaggc aaggacagcc gttactatct tagccgcggc cagatgatca
     5701 tcctgggcgg tacatttacc ttggcgtcca tcgtcgtctt tctcatcggc atgatggtcg
     5761 gcaaagaact cgaggagcga aagctcatca aaaaagacga gcctctggtg aagatgcccg
     5821 tcaaaccagc cgggggggcc gctggcacgg cggcgaagaa cgatgaaatt actttctacg
     5881 ataccttagc aaaatcccgc gacgcccagg cgctggccga gccgccggcc a
//
LOCUS       DASQKS010000175         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4713397, whole genome shotgun sequence.
ACCESSION   DASQKS010000175 DASQKS010000000
VERSION     DASQKS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4713397"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..578)
                     /locus_tag="VE170_04400"
     CDS             complement(12..578)
                     /locus_tag="VE170_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HYJ14705.1"
                     /translation="MDSKMAYSLAQFVADLRSITAAAADEPEILRQVAPLAERLAGNP
                     DLPNQAPIDLKVEKGLSFGLLHENPDHTLAVALLRWLPRGATLPHNHGTWGIVVGLEG
                     AEKNIFWRRSDDGSRAGYAQLERVGVLDCELGRAVVLPNSIIHSVENITDEPSVSLHV
                     YGKNVYYTNRSHFDIVNRRELPWSKNPS"
     gene            complement(689..1306)
                     /locus_tag="VE170_04405"
     CDS             complement(689..1306)
                     /locus_tag="VE170_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HYJ14706.1"
                     /translation="MTASWQIIYVDLDDVLCATARHFLAVVEREFDKRIAYDALSDFD
                     IGASCGLRREETEELYRIVHRPDELMQMAPIDGAIAALRQWVESGYEIAIVTGRPPES
                     YEPSLAWLARYRVPHQSFTMVDKYSRFTTDNTVAIRLDELEQREYCWAVEDSLPMAQF
                     LATRMKLPVALIDCPWNRVAEDHANVGRYGDWPTLAAQMPKRLRR"
     gene            complement(1313..1786)
                     /locus_tag="VE170_04410"
     CDS             complement(1313..1786)
                     /locus_tag="VE170_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14707.1"
                     /translation="MKTYVIRADISAQRLAETGPVVSGSWARQHDELGREIRGEFLID
                     TGAYGAMIDLAVAEMLPLSPLGTRAVHGIHGYGTLQLYLGRVYLPAVDLDGKPMLYST
                     VLECVGVPSLREKSYEHRADIIGILGRIFLRNASFTVDHLAGKLELVIAEQPSNV"
     gene            complement(1813..2394)
                     /locus_tag="VE170_04415"
     CDS             complement(1813..2394)
                     /locus_tag="VE170_04415"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYJ14708.1"
                     /translation="MKLPRAFYRQPTKEVAKQLLGKYLVHRYAEDTLVGRIVETEAYL
                     GPHDLACHAAKGRTKRTDVMFGAAGHAYVYFIYGFYNMLNLVTEAKDYPAAVLIRAVE
                     PVRGIEQMKQLRKSELLHNLASGPGKLCQAFAIDRSLNGADLCGQSLYLEDRGEPMPK
                     FTATPRIGVDYAGKWKDKPLRFFVRGSAFVSKG"
     gene            complement(2445..2969)
                     /locus_tag="VE170_04420"
     CDS             complement(2445..2969)
                     /locus_tag="VE170_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14709.1"
                     /translation="MKQLPGVIARCAVLGTLLWLTACESEDFLKVESVRSPLMEDFES
                     YASILEVTAKLPKNLEVKVVADTSLAKNGSQPPYKIHSISVAPYEHLNHLGTLQLTFY
                     NDRLMQSAFYPEKFKSYVSAVAPTGWVLQLGAETVRGNTVIWQGKDDDQYRFVGWADK
                     RLRQQQQRWLVRYN"
     gene            complement(2966..>3364)
                     /locus_tag="VE170_04425"
     CDS             complement(2966..>3364)
                     /locus_tag="VE170_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ14710.1"
                     /translation="IGASMLFGSCLCGGVKYEIDGALGAVTNCHCSLCRKMSGSAFAS
                     GAAIPATAFRFTAGQELLKEWQSSPGNHRVFCGRCGTPLVKRKDKDPSSLRFRPGSLD
                     NDPDVKISKHLHVSSKAPWVEIKDGLPQEG"
BASE COUNT          722 a         1000 c          907 g          735 t
ORIGIN      
        1 acctccccag ctcatgaagg gttcttgctc catggtagtt cgcgccgatt gacgatatcg
       61 aagtgcgagc ggtttgtgta gtaaacattc ttgccgtaga catgcagcga tacgctcggt
      121 tcgtccgtta tattttcgac actatgaatt atcgagttcg gcaacaccac ggctcgtccg
      181 agctcgcaat ccaagactcc gacccgttcg agttgtgcat aaccggcgcg gctgccgtca
      241 tcgctgcgcc gccagaaaat gtttttctcc gcgccttcga gaccgacgac gataccccaa
      301 gtaccgtgat tgtgcggcaa cgtcgcgccc ctcggcaacc aacgcaacag cgccaccgcc
      361 agcgtatgat ccggattttc atggagcaga ccgaagctca agcctttttc gaccttcaaa
      421 tcgatcggcg cctggttcgg taagtctggg ttgccggcca accgctcggc cagcggcgca
      481 acctggcgga ggatctccgg ttcgtccgcc gcggcggccg tgattgagcg caggtcggcg
      541 acaaattgcg ctagtgaata agccatcttg ctgtccattg aaattaaatt taccgcacct
      601 gcgaaattgt tctgagttcc tcctgttacg ctggcagcaa gtccgattcc tttttttgtg
      661 aactttgcgt tctttgcggc caatcttcct atcttcttag gcgcttcggc atctgcgccg
      721 cgagcgtcgg ccagtcgcca tagcggccga cattggcatg atcttcggcg acccgattcc
      781 acggacaatc gatcagcgcc acgggaagct tcatccgagt ggcgagaaac tgcgccatcg
      841 gcagggagtc ttccaccgcc caacaatatt cccgctgctc cagctcgtcg agacgaatcg
      901 ccacggtgtt gtcggtcgta aagcgcgagt atttatcgac catggtaaaa gattggtgcg
      961 gcacgcgata acgcgcgagc cacgccaatg acggttcata ggactcgggc ggcctgccgg
     1021 tcacgatggc aatctcatag ccgctctcca cccattgccg cagcgcggcg atggcgccgt
     1081 cgataggcgc catttgcatg agctcgtccg gccgatggac gatgcggtag agctcttcgg
     1141 tttcctcccg ccgcagaccg cacgaagcac cgatgtcaaa atcgctgagt gcgtcatagg
     1201 cgatccgttt atcgaattcg cgctccacga cggcaaggaa atggcgcgcg gtggcgcata
     1261 gcacgtcatc gagatcgacg tagataattt gccagcttgc ggtcatgggc agctaaacgt
     1321 tgctcggttg ctcggcgatg accagttcga gttttcctgc cagatgatcg actgtgaaac
     1381 tggcgttgcg caagaatatc cggccaagga tgccaatgat atcggcgcga tgctcgtaac
     1441 tcttttcacg cagggacggc acgccgacgc actcgagcac ggtcgaatac agcattggct
     1501 tgccgtccag gtccaccgcc ggcaaataaa cgcggcccaa gtaaagctgt agggtgccat
     1561 aaccatggat gccatgaacc gcccgggtgc cgagcggcga caggggtagc atctcggcga
     1621 cggcgagatc gatcatcgca ccgtaggcgc cggtgtcgat gagaaattcg cctctgatct
     1681 cgcgacccag ttcgtcatgc tgccgcgccc agcttcccga caccaccggc ccggtctcgg
     1741 ctaaccgttg tgcgctgatg tccgctcgaa ttacgtaggt cttcatcgag aattctgtcc
     1801 gaccgagttg gtttagcctt tagagacaaa tgcgctacca cgaacaaaaa aacgtaacgg
     1861 cttgtccttc cacttaccgg catagtctac accgatccgc ggcgtggcgg tgaatttcgg
     1921 catcggttcg ccgcggtctt cgaggtaaag actctggccg caaagatcgg cgccattgag
     1981 cgatcggtcg atggcgaacg cctggcaaag cttgcccgga ccgctggcga gattgtgcag
     2041 caattcactc tttcgaagct gcttcatttg ctcgatcccg cgcaccggct ccaccgcccg
     2101 gatcaatact gccgccgggt agtccttcgc ctcggtgacc agattgagca tgttgtagaa
     2161 tccgtagata aagtaaacat aggcgtggcc cgcggcgccg aacatcacat ccgtccgctt
     2221 ggttcgccct ttagcagcgt gacaagcgag atcgtgcggg ccaagataag cctcggtttc
     2281 gacaatccgt ccgactaaag tgtcttcggc gtatcgatgc acgagatatt tacccaacag
     2341 ctgtttggca acttctttgg tcggctgtcg ataaaacgcg cgaggtaact tcacgacaag
     2401 agaatcgtca tcaagcgaac taaattgaac ccgaagtgga ccgatcagtt gtagcgcacc
     2461 agccagcgct gttgttgttg gcgcaagcgt ttatcggccc aaccgacaaa tcggtactgg
     2521 tcgtcgtcct taccttgcca gatcaccgta ttaccccgca cggtttcggc gccaagttgc
     2581 agcacccagc cagttggcgc aaccgcgcta acgtaacttt tgaatttctc tggatagaaa
     2641 gcgctttgca tgagccggtc gttgtaaaaa gttagctgca aggtgcccaa gtgattcaaa
     2701 tgctcgtaag gcgcgacgct gattgaatga atcttataag gcggttgaga gccatttttt
     2761 gccagggagg tatcggcgac caccttcact tccagattct tgggcagttt cgccgtcacc
     2821 tctaggatcg atgcgtagct ctcaaaatcc tccatcaacg gtgaacgaac gctttcaact
     2881 ttaagaaagt cctccgactc gcaggccgtc agccacagca aagtgccaag cacagcgcag
     2941 cgagcaatga cccctggcaa ttgtttcatc cctcctgcgg caaaccatcc ttgatttcga
     3001 cccacggcgc cttcgagctg acgtgaaggt gtttggatat tttcacatcc ggatcgttgt
     3061 ccaaactgcc gggacggaag cgtaagctgc tcggatcttt atctttccgc ttcactaacg
     3121 gcgtgccgca gcggccgcaa aatacccgat gattgcccgg cgacgattgc cattccttga
     3181 gcagctcttg tcccgccgtg aagcgaaagg ccgtcgccgg aatcgccgct ccgctggcaa
     3241 acgccgagcc cgacatcttg cggcacaaac tgcagtgaca gttggtcacc gcgccaagag
     3301 cgccgtcgat ctcatatttc acaccgccgc aaagacagga tccaaacagc atggaagctc
     3361 cgat
//
LOCUS       DASQKS010000176         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5554521, whole genome shotgun sequence.
ACCESSION   DASQKS010000176 DASQKS010000000
VERSION     DASQKS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5554521"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VE170_04430"
     CDS             <1..1051
                     /locus_tag="VE170_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYJ14711.1"
                     /translation="AFVNSVRGSLLAWVDQMVTADIIISSGARTAGPRNVSLHEEPAA
                     GLRALPGVKIVDLYRLVRANYQGRPIVVESFSAAESASVRTLPMAQGDGARALREMAE
                     AKGAIVSESFQTRFAKQVGDSFELTTPSGPVSFKVLGIYIDYSSDIGSVLIDRALYKK
                     YWRDELVDAFDLWLEPGVDMAALIAKIKAGYGEKYQLFISTHRELKAAVLRIMEQSFV
                     VNYAVEIVALVVAIFSVINTLLASILDRSREIGVLRAIGATQAQVRRIIVLEAGWMGL
                     IGAILGLFAGSVMAYHHVVYNTKLLTGWTFQFFYPYDVAGLVIAATVLLCLLAGYGPA
                     KRAAATPVVTAIGYE"
     gene            complement(1243..1692)
                     /locus_tag="VE170_04435"
     CDS             complement(1243..1692)
                     /locus_tag="VE170_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ14712.1"
                     /translation="MPQSLSGSSTPASARSLSSMEAISRGVAAVTPPGAALKDVYYKF
                     DSVELEGEALEVLQKNAEWMKANPKARVEVEGHCDDVGSAEYNLVLGAKRAQSAKEFL
                     INQGIAPDRLVTISYGKEAPACFDPAEDCRVRNRRARFVMFTEVPTS"
     gene            complement(2077..3354)
                     /locus_tag="VE170_04440"
     CDS             complement(2077..3354)
                     /locus_tag="VE170_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14713.1"
                     /translation="MLRRIFAKLSGFYFGWRMIAVSCAIRVLGGGLHAYGLSVFFLPV
                     TQELGLTRTATSLVFSLARAQGAIEGPLAGYCIDRFGPRPVIAIAVALAGIGYLMLAS
                     VHNYAMLLVVYMGIISLSYQAGFMDATMALANTWFIRKRALAMSITSGSIALGGALLT
                     PFLAYAIHTWGWRSASIAAGVAFLVVGLPLSLLIQRSPESLGLLPDGAAPAEKISRSD
                     ATETAGQAASESEYTLRETLRTYQFWLLAVATSLRVICSSAVLVHFVPILVWKGYSEP
                     EGAFYLGVIAFCGMPTHLLIGWLGDRHYKPWLMAGFMVLGSLSVYLLFDGTQSWQLWI
                     AVLLFSVFEGLFPVTWATVGDFFGRKNFAKIRGSMSFLYTWGSVLGPVLAGAIYDRTD
                     SYAALLWILFATCWVTAALYAMMWQPKPRTRRS"
BASE COUNT          736 a          954 c         1019 g          661 t
ORIGIN      
        1 cgccttcgtc aatagcgtgc gcggctcgct gctcgcctgg gtcgatcaga tggtgacggc
       61 ggacatcatc atcagctccg gcgcgcgcac cgccggaccg cgcaatgttt cgctgcatga
      121 agagccggcg gcgggactgc gcgctttacc gggagtgaaa atcgtcgatc tctatcgcct
      181 ggtgcgcgct aactatcaag ggcggccgat cgtcgtcgag tctttttctg ctgccgaatc
      241 ggcgtccgtg cgcacattgc cgatggccca aggcgacggc gcccgcgcct tgcgcgaaat
      301 ggccgaggcc aagggcgcga tcgtcagcga gagctttcag acgcgtttcg ccaagcaagt
      361 gggtgacagt tttgaactga ccacaccttc cggaccggtc agtttcaaag tattggggat
      421 ttacatcgat tattcttctg acatcggcag tgtgctgatc gatcgcgcgc tctataaaaa
      481 atattggcgc gacgaattgg tcgatgcctt tgacctgtgg ctcgaaccgg gcgtggatat
      541 ggctgcgctg attgcgaaaa tcaaagccgg ctacggcgaa aagtatcaac tttttatcag
      601 cacccatcgc gagctcaagg ccgctgtgct gcgcatcatg gagcaatcct tcgttgtcaa
      661 ctatgccgtc gaaatcgtcg ctctggtggt ggcgatcttt agcgtcatca acaccctgct
      721 ggcttcgatc ctcgaccgca gtcgcgagat cggcgtgctg cgcgccatcg gcgcgaccca
      781 ggcacaagtg cgccgcatca ttgtcttgga agcgggttgg atgggactga tcggcgccat
      841 acttggcttg ttcgccggca gcgtaatggc ctaccaccat gtggtttata acaccaagct
      901 gcttaccggc tggacgtttc aattctttta tccctatgac gtcgccggcc tggtgatagc
      961 cgccaccgtg ctgctctgcc tgctcgccgg gtacggtccg gccaaacgag cggcggcgac
     1021 cccggtggtg acggcgatcg gttatgagtg aatgtcggat tggtatggcc gcacagaacg
     1081 caaagtactc aaaggtggga aagcaaaaag agaatcacct agcgtgggcg atgacgcaac
     1141 ggttcacaaa tgtatcggaa gggccgccac agagatttgc tcccagaatt ccgattctgt
     1201 tttgtgaact atgcgttctt tgcggccaag tatccgagta tcttacgagg ttggcacttc
     1261 agtgaacatg acgaagcggg cacggcggtt gcgcacgcgg cagtcttcgg cggggtcgaa
     1321 gcaggcggga gcttctttgc cgtagctgat cgtcaccaag cgatcggggg cgatgccttg
     1381 gttgatgaga aattccttgg cggattgagc gcgcttggcg cctaggacga gattatattc
     1441 cgccgaaccg acatcgtcgc agtggccttc gacttcgacg cgcgctttcg gattggcttt
     1501 catccactcg gcatttttct gcagaacctc aagcgcttcc ccctcgagtt cgacactatc
     1561 gaatttatag tagacatcct tcaacgccgc ccccggcggt gtcacggcgg cgacgccgcg
     1621 gctgatcgct tccatcgatg aaagcgaacg ggccgacgcg ggcgtggagc taccgctgag
     1681 cgattgcggc atggaaaaca gcccggcctt gggcgactcc gacgatgagc agccgaagct
     1741 aaacaatagg gtgacaagca ggaatctgag cgcacgcgcg tggcggcttg tcggcgtcga
     1801 gttcgtaccc gatggcgtga atatgtgcat taaactggct atatcctcgc gatttggtcc
     1861 gaagttgtca agtttttttt cccgtctgtt ggccgacctt ccggtctccg tgattacggt
     1921 cctgatgcgg tccaaattgt gccaaaaatt gcgcagtagc cagtgagcgc catacgcggg
     1981 cactggcgtg taggccccgc atgaaggcga accatccttc accgaggttt gcggaataag
     2041 atgctcgacc caataacaga gtcggtgtag cgcccgtcac gaacgccggg tccgaggctt
     2101 gggttgccac atcatggcgt aaagggcggc ggtgacccaa caggtggcaa aaagaatcca
     2161 caacagtgcc gcgtagctat ccgtgcggtc gtagatcgcg cctgcgagca ccggacccaa
     2221 gacactgccc caagtgtaga gaaagctcat gctgccgcga atcttggcga aattttttcg
     2281 cccaaagaaa tctccgacgg tcgcccaggt aaccggaaag agaccttcga agacactgaa
     2341 gagcaacacg gcgatccaca attgccagct ctgtgtgccg tcaaataaga gatagaccga
     2401 caaactgccg agcaccatga aaccggccat gagccagggt ttgtaatgac gatcaccgag
     2461 ccaaccgatc agcaaatgtg tcggcatgcc gcaaaaagca atgacgccca ggtagaatgc
     2521 tccttcgggt tcgctgtatc ccttccacac caggatcggc acgaagtgga ccagcacggc
     2581 gctggagcag attacgcgca agctggtggc gacggcaagc aaccaaaact gataggtgcg
     2641 caatgtctcg cgcaaggtgt actcggattc gctcgcggct tgtccggctg tttctgttgc
     2701 atcgctgcgg ctgatctttt cagcgggtgc ggcgccatcg ggcaacagac cgaggctttc
     2761 cggcgaacgc tggatcagca gcgacaacgg caaaccaacg accagaaacg cgacgccagc
     2821 cgcgatggat gcgctgcgcc aaccccaggt gtgtatcgca taggcgagaa aaggtgtgag
     2881 cagggcgccc cccaaggcaa tcgacccgct ggtgatcgac atggccaggg cgcgcttgcg
     2941 gatgaaccag gtattggcca gcgccatggt ggcgtccatg aagcccgctt ggtacgacag
     3001 cgagatgatg cccatgtaga cgaccagcaa catcgcatag ttgtgcacgc tggcgagcat
     3061 gagataaccg atgccggcga gggcgacggc gatggcgatc accgggcgcg ggccgaaacg
     3121 atcaatgcaa tagccggcga ggggaccttc gatggcgccc tgggcgcggg cgagggaaaa
     3181 taccaacgaa gtggccgtgc gcgtcagtcc cagttcttgc gtcaccggca aaaaaaacac
     3241 cgacaatcca taggcgtgca agccgccgcc gagcacgcgg atcgcgcaac tgacagcgat
     3301 catgcgccag ccgaaataaa accccgagag tttggcaaaa atgcggcgca gcatcgggga
     3361 attttaggct
//
LOCUS       DASQKS010000177         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_953484, whole genome shotgun sequence.
ACCESSION   DASQKS010000177 DASQKS010000000
VERSION     DASQKS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953484"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..987)
                     /gene="tatC"
                     /locus_tag="VE170_04445"
     CDS             complement(214..987)
                     /gene="tatC"
                     /locus_tag="VE170_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYJ14714.1"
                     /translation="MTTPDGKGHHADVQMPFTSHLGELRSRLIKSVLAIAIAFSACFY
                     FVDTIFAILAAPVRRLNIPGLTIIGTGVTEAFFTRMKLGFVAALVVALPVLLWQGWQF
                     VAPGLYEHEKRHTRSFVFAGSLCFCLGAAFSYAVVVQHGLNFLLHRYEVIGVTPMLQI
                     ADYLSTVSRLLLVSGAMFELPVIAFFTARVGLIDHRFLIRHSRYALIAIALLAAILTP
                     PDLISQILLMVPLLLLYGLSIAVAYLARFRMDRRERAAQ"
     gene            complement(984..1259)
                     /locus_tag="VE170_04450"
     CDS             complement(984..1259)
                     /locus_tag="VE170_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYJ14715.1"
                     /translation="MFGIGMPELLMILGLALIVLGPKKLPELAKTLGKGLAEFRRATD
                     ELKDEFRKVEADLDEPPPPAEPVTTDPVPAQSAEAPQTDANPNAEKK"
     gene            1582..2607
                     /locus_tag="VE170_04455"
     CDS             1582..2607
                     /locus_tag="VE170_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HYJ14716.1"
                     /translation="MAKTAVVVDKEYLKHQPGESHPERPERIKVLLDLAGGLDPEKFQ
                     LLPPQPAKRADLIATHGEDHVKLIEATARHNRYALDGDTITSRDSFAVSLLAVGGFIG
                     LLDAIAAKDCRNGFALVRPPGHHALRNHAMGFCLFNTMAIGAEYLQRTYGAKKILIMD
                     WDVHHGNGTQDAFYADPSVLFISSHQYPYYPGSGAANEVGSGAGEGFTINIPLPAGCA
                     DPEYAQAFHDIVVPAAEKFAPDWILVSAGFDPHRRDPLGGMGVTDGGFALMAHRLLGL
                     ADKFTDGKIAFLLEGGYDLAGLRGSVAAVLAAMQSAAVPAVDAAQLGASQIAPLIRHI
                     RELHERY"
     gene            2689..3939
                     /gene="fabF"
                     /locus_tag="VE170_04460"
     CDS             2689..3939
                     /gene="fabF"
                     /locus_tag="VE170_04460"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYJ14717.1"
                     /translation="MNSTEARRVVVTGIGLVTPLGTGVEKNWQALMAGRSGIRKISRF
                     NADAFASRIAGEVPDFKAEDYIEAKEIKKMDLFIHYAMGASAQAMADSGLKIEGEFAE
                     NVGVIIGVGMNGLDTLEVTKEALMAGGPRKISPFFIPKVISNLAPGHVAIRYGAKGIN
                     LTPTSACASGTHAIGEAYHMIRRGLQDAVIAGGAEAAITPLGVGGFAAMKALSTRNDE
                     PERASRPFDKERDGFVIGEGSGILILEELQHALERGAKIYAEVLGYAANGDAYHMTAP
                     APEGEGAARCMRLALKDAGIAPSDVSYINAHGTSTEYNDANETTAIKTVFGEAAATVA
                     VSSTKSMTGHLLGAAGAVEGVYSALALHHGMLPPTINYENPDPACDLDYVPNHARKAD
                     VKIVLSNSFGFGGTNACVIFGRCA"
     gene            3936..>4355
                     /locus_tag="VE170_04465"
     CDS             3936..>4355
                     /locus_tag="VE170_04465"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HYJ14718.1"
                     /translation="MSRSERRSVILGTGSELPSKIVTNHDLEQIVDTSDEWITVRTGI
                     KERRILEDGKGNADMAYEAAKRALNDAHVDAQDLDAIIMGTVSADYPFPSSACVLEDK
                     LGARNAFSFDVGAACAGFLNALAVGDLFVRSGKANKAL"
BASE COUNT          934 a         1247 c         1293 g          881 t
ORIGIN      
        1 ttcaggcttt tgttgggcag cgtgtcggct tccgctcgtt tgtttgcagc aaaattagcc
       61 ttcaaccgcg cccaggcaag ctccgtacgc gctgggttcg gacgattgac ttagagggaa
      121 aatctttcgc cgcgacaacg aacgcttgag ctagttcgga tcatgattca cccgttggtg
      181 taaatatctt tacagtaagc caggaagaaa tatttactgc gccgcccgtt cccgccggtc
      241 catgcggaag cgcgccaggt aggcgacggc gatgctgagg ccgtagagca atagcagcgg
      301 caccatcagc aagatttgcg agatcaaatc gggcggcgtc aggattgccg ccaagagcgc
      361 gatggcgatc agagcgtagc ggctgtggcg gatcaagaag cgatggtcga tcaggcccac
      421 ccgagcggtg aaaaaagcga tcaccggcag ttcgaacatg gcgccggaga ccaaaaggag
      481 ccgggacacc gttgacagat agtcggcgat ttgcagcatc ggcgtgacgc cgatgacctc
      541 ataacgatgc agcaaaaaat tcaatccatg ttgcacgacg accgcgtaac tgaaagccgc
      601 tccgaggcaa aagcacagtg aaccggcgaa gacgaaactg cgcgtatggc gcttctcatg
      661 ctcgtacagg ccgggggcga cgaattgcca accctgccac aagagcaccg gtagagcgac
      721 caccagcgcc gcgacgaagc cgagtttcat tctggtaaag aacgcttcgg ttaccccggt
      781 gccgatgatt gtcagccctg gaatgttgag acggcgcact ggcgcggcga gtatcgcgaa
      841 tatggtgtcg acgaaataga agcaggctga gaacgcgatg gcgatggcga ggaccgactt
      901 gatcagccgc gagcgcaatt ctcccaagtg cgaggtaaag ggcatttgga catcggcgtg
      961 atgccctttg ccgtccggcg ttgtcacttt ttctcggcgt tgggattggc gtcggtttgc
     1021 ggcgcttcag cggattgcgc aggaacggga tcggtcgtca ccggctcggc tggcggcggc
     1081 ggctcgtcaa gatcggcctc cactttgcga aattcatcct tgagctcatc ggtggcgcgg
     1141 cgaaactcgg ccaaaccttt gcccaaggtc ttggccagtt ccggcagctt cttgggtccg
     1201 agaacgatca aagccagtcc caagatcatg agtaattccg gcatgccgat accaaacatg
     1261 caactaagct aagcgatttc attcggctcc gcaagattgc gcggggacca cgtttgtttg
     1321 ccgagcgaga gtgaatttct cgctgctcct aaccgacaac cgcacagcgc cgcagcgaag
     1381 caccccaaag agcatgcgca ttcggaaaat cagcggcaac gaacccaaaa gcgatgcacg
     1441 aagcagggtg tgaaacccgc cccggttggt taccaaagaa aaatattaag aacgttgtgg
     1501 tcccaagtga agcgcgggat cgcgaccgaa ttgcggcgaa attgctttgc agcgcctggg
     1561 cgtcggtgtt aggttcttat tatggcgaag acggcggtgg tagtcgataa agagtatctg
     1621 aaacaccagc ccggcgagtc tcatccggag cggccggagc gcatcaaagt gttgctcgac
     1681 ttggcgggcg gtctcgatcc cgagaaattt cagttgctgc cgccccagcc tgccaaacgc
     1741 gccgatctca ttgccaccca tggagaagat cacgtcaaac ttatcgaagc gacggcgcgg
     1801 cacaatcgct acgccttgga cggcgacacc atcaccagcc gcgattcgtt tgctgttagt
     1861 ttattggccg tgggcggatt cattggtctg ctcgatgcga tcgctgctaa agattgccgt
     1921 aacggttttg ctctggtgcg gccgccgggc catcatgccc tgcgcaacca tgccatgggg
     1981 ttttgtctgt tcaacaccat ggcgatcggc gcggaatatt tgcagcgcac ctacggcgcg
     2041 aaaaaaattc tcatcatgga ttgggatgtg caccacggca acggcacaca ggatgctttt
     2101 tacgcggacc cctcggtgct gtttatttcc agccatcaat atccctatta tcccggcagc
     2161 ggtgcggcca acgaagtcgg cagcggcgcg ggcgaaggat ttacgatcaa tattccgctg
     2221 cccgccggtt gtgccgatcc ggaatacgcc caggcgtttc acgatatcgt cgtaccggcg
     2281 gcggaaaaat tcgcacccga ttggattctg gtgtccgccg gtttcgatcc gcaccggcgc
     2341 gatcccctcg gcggcatggg ggtgacggat ggaggtttcg cgctcatggc acaccgctta
     2401 ctcgggctcg ccgacaaatt caccgacggc aaaatcgctt tcctgctcga aggcgggtat
     2461 gatctcgccg gtttgcgcgg ttcggtggcg gcagtgctgg cggctatgca atccgccgcc
     2521 gtcccggccg ttgacgccgc ccaattgggt gccagtcaga tcgccccgct gattcgccac
     2581 attcgagagc ttcatgagcg ttattgatga cttgacagtc ctattgattc accggtaagt
     2641 ctcctttatc gtgatgtttc aaatccatta aagattggtt aggtatttgt gaactcaact
     2701 gaagcacgcc gagttgtcgt gacgggtatt ggtctggtca cgccattggg taccggagtc
     2761 gaaaagaatt ggcaggcgtt gatggccggc cgctcgggta tccgcaagat cagccgcttc
     2821 aacgctgatg cgtttgcctc gcgtatcgcc ggcgaagtgc ctgacttcaa agccgaagat
     2881 tatatcgaag cgaaagagat caaaaaaatg gatctcttca ttcactacgc catgggtgct
     2941 tcggcccagg ccatggccga cagcggtctt aaaatcgaag gcgagttcgc cgagaacgtc
     3001 ggcgtcatca tcggcgtcgg catgaacggc ctcgatacgc tcgaagtcac caaagaagcg
     3061 ctgatggccg gcgggccgcg caagatatcg ccatttttca ttcccaaagt catttccaat
     3121 ctagcccctg gccatgtggc gattcgttac ggcgccaagg gcatcaattt aaccccgacc
     3181 tcggcctgtg cttcgggcac ccatgcgatc ggcgaggcct atcatatgat tcgccgcggc
     3241 ttgcaggatg cggtgatcgc cggcggcgcc gaagcggcga tcacaccgct gggcgttggc
     3301 gggtttgccg cgatgaaagc gctgtcgacg cgcaatgacg agcccgagcg cgccagcagg
     3361 cccttcgaca aagagcgcga cggcttcgtc atcggcgagg gctccgggat tttgatcctc
     3421 gaagaactcc agcatgcgct cgagcgcggc gccaaaatct acgccgaagt gctcggctac
     3481 gcggctaacg gcgatgccta tcatatgacc gcgccggcgc ctgagggcga aggagcggcg
     3541 cgctgcatgc gcttggcctt gaaagacgcc ggcattgcgc cgagcgacgt cagttacatc
     3601 aatgcccacg gcacgtcgac ggaatataac gacgccaacg aaaccacggc gattaaaact
     3661 gttttcggcg aagccgcggc gaccgtggcg gtgagttcga ctaagtcgat gaccggccat
     3721 ttgctcggcg ccgccggcgc ggtggagggc gtctactcgg cgctggcgct gcatcacggc
     3781 atgctgccgc cgacgatcaa ctatgaaaat cccgatccag cttgcgatct ggactatgtt
     3841 cccaaccatg cgcgcaaagc cgacgtcaag atcgtgctgt ccaactcctt cggttttggc
     3901 ggcaccaacg cctgcgtgat tttcgggagg tgcgcatgag ccgttccgaa cgccgcagcg
     3961 tgatcctcgg taccggctcc gaactgccgt cgaaaatagt caccaatcat gacttggaac
     4021 aaatcgtcga caccagcgac gagtggatca ccgtgcgcac cggcatcaaa gaacggcgga
     4081 ttcttgaaga tggcaagggc aatgccgata tggcctatga agccgccaag cgcgctctca
     4141 acgacgctca cgtcgatgcg caagatctcg atgccatcat catgggcacg gtttcggccg
     4201 attacccttt tcccagttcc gcctgtgtgc tcgaggacaa gctcggtgcg cgcaatgcct
     4261 tttcctttga tgtcggcgcc gcctgtgccg gctttctcaa tgcccttgcc gtgggcgatt
     4321 tattcgtgcg cagcggcaaa gctaataagg cactg
//
LOCUS       DASQKS010000178        10289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_514216, whole genome shotgun sequence.
ACCESSION   DASQKS010000178 DASQKS010000000
VERSION     DASQKS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514216"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VE170_04470"
     CDS             complement(<1..330)
                     /locus_tag="VE170_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ14719.1"
                     /translation="MAQEKYETHEYDVVVIGAGGAGLRAAVEASAQGVKTALVCKSLL
                     GKAHTVMAEGGIAAAMGNVYPEDNWKVHFRDTMRGGKLLNNWRMAQIHAQESPDRVLE
                     LEEWGALF"
     gene            complement(330..1094)
                     /locus_tag="VE170_04475"
     CDS             complement(330..1094)
                     /locus_tag="VE170_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HYJ14720.1"
                     /translation="MADKSLTLDNFRTLRKDNWWAEPLLVVVVLGGFGVYATWAAMQN
                     AYYYAAPYLSPFYSPCISANCVHVTLPLIGSWWNLSPAFLILWVPGGFRATCYYYRKA
                     YYRSFFLSPPACAVRDAAKSYAGETRFPFLLQNIHRYFFYLSLVILAFLWWDALLAFR
                     FPDGFGMGVGTIVLWVNAALLTLFSLSCNSCRHVCGGYLNSFHSAPKKFKTWTFISGL
                     NEKHMEYAWVSLVWVALTDLYVRLLAMGVISDLRFF"
     gene            complement(1308..1616)
                     /locus_tag="VE170_04480"
     CDS             complement(1308..1616)
                     /locus_tag="VE170_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HYJ14721.1"
                     /translation="MIERHQPYVRQLHVCINDRKGESKSCGYDDTEEIVEELRQVAKE
                     RNLKGKVRVVRSGCLDVCAFGPNMMIWPEGLWFMKVTKADVPHIVEKYLTLEAAEQPK
                     "
     gene            complement(1864..2760)
                     /locus_tag="VE170_04485"
     CDS             complement(1864..2760)
                     /locus_tag="VE170_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYJ14722.1"
                     /translation="MPDTSPTLPTLKPKRETLMPIQMDSKPLDDSPTPITRIKTTRMV
                     AVCQSLARAALQTNDVMQVFDMAVADVGKVLNIDMVRILLFDDESATSNPKAQFARTA
                     KSAAAYPAQFGEEIVARVAASGKSAIFENIGEQSFLDCFQIAGTTGQYACGFFAAFPM
                     RGKLSRSGILVCINRRPRELSAIESNFIEAIVGQLTVALDYFRLSESLETVQPDRRGG
                     SNDYDRQIPSQAQIAAFVSCEVRSRLTSILGYVQLLQEQFLGGINAKQADALDTIASE
                     SGSLLETLISSIEGRPDIVPHN"
     gene            3062..3745
                     /locus_tag="VE170_04490"
     CDS             3062..3745
                     /locus_tag="VE170_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYJ14723.1"
                     /translation="MIGVDELNACIIGCRKCPRLVGWREACAKKPPRRYVGEDYWAKP
                     LAGVGDPRARVLIVGLAPAAHGGNRTGRMFTGDRSGDWLYGALYQAGFANQPNSDHRD
                     DGLKLIDCYVTAAVHCAPPANKPNLVEFKRCRPYLIEELRIFSTARVVIVLGKVAFDS
                     LLAAYRETGGSLSKPRPKFGHGASYRLANGVRLICSYHPSQQNTFTGKLTRAMFKSVF
                     DQARAELLA"
     gene            4014..6890
                     /locus_tag="VE170_04495"
     CDS             4014..6890
                     /locus_tag="VE170_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYJ14724.1"
                     /translation="MKAGRDYFEIEAALKKRIDGDLHFDQYSRLLYSTDASIYQIEPI
                     GVVVPRHKGDVEAAIEVARRLGVSILSRGGGTSLAGQAVGHSIVLDFSKYMRNVLEVN
                     QEELWCRVQPGLVQDELNAYVRGQGLQFGPDTSTSNRATIGGMVGNNSSGAHSLTYGK
                     TLDHVIELTVLFADGSEVVLKDLAPEVVEGKSKANTIEGHAYREVSRLAQQHKSEIVA
                     RYPKIMRRVSGYNLDEFVKPQPFNLSRMIVGSEGTLAAIVEAKMRLVAKPKWTAMNVI
                     HFNDDIDALECAQIILTTKPYAMESTDKMILNLARGNIEQSRKLGFVQGTPDSLLMVE
                     YAGETEAEVRAQVETLEELRKREKIGYAATLAFKAEEVKSIWGVRKAGLGLLLGMKGD
                     KKPIAFVEDTAVAPAKLPEFIKRFREIVTRHDTVAGYYGHCSVGCMHIRPLINLKENG
                     EKQKMVSIANAISDLVLEFNGAMSGEHGDGLARSHFNAKLFGPALYEGFRQIKRAFDP
                     NNLFNPGKIVDAPAMTESLKISPQYKTWEPQTTLDFTEQGGFARAVEMCSGMGECRKK
                     LDGTMCPSYMGTLDEEHSTRGRANALRNVLSGLVPKAEFTGKRLYDVMDLCLECKACK
                     AECPSNVDMAKLKYEFLDHYHRANGLPLRNRIFGGIASLNRIGSALAPVSNWLANSLP
                     NRWLMDTLAGIDRRRPLPQFAGESFEDWFRVHKPEGDGSKGDVVLFHDTFNNYNTPNV
                     AIAATRFLEKSSYRVLLVDKKCCGRPMISKGMLGQAKAHAAWNIAQLAPYAQKGTAIV
                     GLEPSCLLTLRDEYPEFIRTDAAKKVAQHSFLLEEFVMREVNAGRLSLGAHGKGQKVL
                     LHSHCHQKALVGSAPTIAMLKAAGYEVSEADSGCCGMAGSFGFEKEHYDLSVKIGNRR
                     LAPAVKAASIDIEVVAPGISCRQQIDHLAARKAKHPAELLVPHQ"
     gene            complement(7043..7897)
                     /locus_tag="VE170_04500"
     CDS             complement(7043..7897)
                     /locus_tag="VE170_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HYJ14725.1"
                     /translation="MRLDNEPESQNVEDRRGSRVTRGAVGGGLGTVVLVLIAMFFGID
                     PSAILQGDPQSNVSNEPIPQAPQTGRDEGREFVSRVLGSNERVWGEIFQKAGREYAQP
                     KLVLFSGEVQSACGFAQAAVGPFYCPNDQKVYIDLSFYRELRDRFKAPGDFAQAYVVA
                     HEVGHHVQNMLGIMGKVQARQQRASEREANALSVALELQADCLAGIWASFANRDRKIL
                     EPGDIEEGLNAAAQIGDDTLQKRSQGYVVPEGFTHGSAEQRMRWFRKGIETGDLKQCD
                     TFAGRGVL"
     gene            complement(8027..8347)
                     /locus_tag="VE170_04505"
                     /pseudo
     CDS             complement(8027..8347)
                     /locus_tag="VE170_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            complement(8713..9153)
                     /locus_tag="VE170_04510"
     CDS             complement(8713..9153)
                     /locus_tag="VE170_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HYJ14726.1"
                     /translation="MGSLLNLLPNSKRAGVYRTAIDSDAIVAAAKTAELQVFKIDLAK
                     ARGKSGLFDAFSRTLKFPKHFGKNWDALNDCLTDLAWLDGNGWVLILTNCKPFVVKYQ
                     ADFDLALELLEGVSASWQEDGKPFWLFIPDQPGWSADLPEIALG"
     gene            complement(9158..9490)
                     /locus_tag="VE170_04515"
     CDS             complement(9158..9490)
                     /locus_tag="VE170_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HYJ14727.1"
                     /translation="MVLLVGGGTSCAREPSDRQADQVLLTELPREAHKTLSLIKKDGP
                     FPYHRDGATFGNFERRLPQKERGYYKEYTVPTPGARDRGARRIVAGRKGEFYFTDDHY
                     QTFRRILE"
     gene            9892..>10289
                     /locus_tag="VE170_04520"
     CDS             9892..>10289
                     /locus_tag="VE170_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14728.1"
                     /translation="MEQARTIVRLWADRHRQAVMYDDDSAVLLDVASGKRLKIAWPAL
                     VSYEEKVHPETQEPYIVLLFDDGTQLALVDPGGVAFAPLTVNTGALENLPAVVCLRDL
                     LLLKQRVDHFLYGHADEPPPRECLNLIMIC"
BASE COUNT         2231 a         2767 c         2929 g         2362 t
ORIGIN      
        1 gaagagcgcg ccccattcct ccagttcgag tacccgatcc ggcgattcct gagcgtggat
       61 ttgcgccatg cgccaattat tgagtaattt gccgccgcgc atggtatcgc gaaagtgaac
      121 cttccagttg tcctccggat agacgttgcc cattgccgcg gcgataccgc cttcggccat
      181 tacggtatgc gccttgccaa gcaaggattt gcacaccaga gcggttttga cgccctgggc
      241 tgaagcctct accgctgcgc gcaggccggc gccgccggcg ccgatgacca cgacgtcgta
      301 ttcatgcgtt tcgtactttt cttgagccat tagaaaaacc tcagatcgga aatcacgccc
      361 atcgccagca gccgcacgta gaggtcggtc aaagccaccc agaccagact gacccaggca
      421 tattccatat gtttctcgtt aagaccgctg ataaaggtcc aggtcttaaa ctttttcggc
      481 gcactgtgaa atgagttcag atatccgccg cagacatggc ggcaggaatt gcacgacagg
      541 ctgaacaacg tcaacagcgc ggcgttgacc cacagcacga tggtgccgac gcccatgccg
      601 aagccatcgg gaaaacggaa tgcgagcaac gcgtcccacc agagaaaagc caggatcacc
      661 agcgaaagat agaagaaata ccggtgaata ttttgcagta aaaatgggaa acgggtttca
      721 ccggcgtagg atttagcggc atcgcgaacg gcgcaggccg gcggcgatag gaagaacgag
      781 cgatagtagg ctttgcgata atagtagcag gtcgcgcgga agccgcccgg aacccagagg
      841 atcaagaaag ccggcgatag attccaccag gaaccgatca gcggcagggt gacatggacg
      901 cagttggctg agatgcatgg cgaatagaac ggcgacagat agggggcggc ataataatag
      961 gcattttgca tggccgccca agtcgcgtag acgccaaaac cgcccaacac cacgacgacg
     1021 agtaacggtt cagcccacca attatctttt cgcaacgtgc gaaagttatc gagagtaaga
     1081 cttttgtcag ccacggtgat ggttccttat gcttgttaat tatttcaatg ccgattcact
     1141 attatggaaa tatggaaatc ggttaagagt caagtaagct tacccgcacg cgccgacggc
     1201 aggccggatg gcgtaccaac gcggcttcag cgtaagcaaa gccgtgtcta atcgattgtt
     1261 agcttcgatc ggaagtggcg agcggcgtgt tatgcaacgt cgctggttta cttcggctgt
     1321 tcggcggctt cgagagtcaa atatttttcg acgatgtgcg gtacgtctgc cttagtcact
     1381 ttcataaacc aaagaccttc cggccagatc atcatgttgg gaccgaaggc gcagacatcc
     1441 aaacaaccgc tgcggacgac gcggactttg cccttaaggt tacgttcttt ggcgacctgg
     1501 cgcagttcct cgacgatttc ttccgtatcg tcatagccgc aggatttgct ttcgcctttg
     1561 cggtcgttga tgcaaacgtg aagttgccgc acataaggct gatgccgttc gatcattcaa
     1621 actcccttcg aactttgatt tttggcaatt ttataacaga aaaagaaaac caatgcacgc
     1681 ggttctacgc tggtgagctt gtcgccgctc gccttgctaa atcgccggtt ggcattatct
     1741 ggacaaagga ataacgtctg ttccaaacca ggatcagatc accgagaatc ttagaaatta
     1801 ttattatctt taggcggcgt gatcgaaccg cgctggcgcc ggcgattgaa aacagtcggc
     1861 tggctaatta tgcggcacga tatcgggacg accttcgatg gagctaatta atgtttccag
     1921 caagctgccg gattctgagg cgatcgtatc cagggcatcg gcttgttttg cgttgatgcc
     1981 gccaagaaat tgctcctgaa gtaactgaac gtaaccgaga attgaagtta gccgtgatcg
     2041 gacttcacaa gaaacgaacg cggcgatttg cgcttgagac gggatttgcc ggtcgtagtc
     2101 gttgcttccg ccacgccgat ccggctgaac ggtttccagg ctttcgctca gtcggaaata
     2161 atctagcgcc accgtcaact gaccgacgat agcctcgata aaattgcttt cgattgcgct
     2221 gagctcgcga ggtcggcggt tgatgcagac caatattccc gaacggctca atttgccgcg
     2281 catgggaaag gcagcaaaaa agccgcaagc atattgaccg gtggttccgg cgatttggaa
     2341 gcaatccaaa aatgattgct ctccgatgtt ttcgaaaatc gctgatttcc cggatgcggc
     2401 gacccgtgcc actatctctt cgccgaattg cgctgggtag gctgccgcag actttgctgt
     2461 acgggcaaat tgtgctttag gattcgatgt cgccgactcg tcgtcgaaga gaagaattcg
     2521 caccatgtcg atgttcaaaa ctttaccgac gtcggccact gccatatcga aaacttgcat
     2581 gacgtcgttg gtttgaagcg ccgcccttgc caatgattga catacagcca ccatgcgagt
     2641 cgtcttgatc ctcgtgatcg gtgtcgggct atcgtccaaa ggttttgaat ccatttggat
     2701 aggcataagc gtctcccgtt ttggcttaag cgttggcaat gttggcgatg tgtctggcaa
     2761 cgctatatta agtttacaat tacggctatg gggtaaatgc tgtataaggc ggagaaaaat
     2821 tgcttagtgg cgattgtcgt ggggtctctg ccatggcgca aataggggct gaacgaagcg
     2881 gctgccgctg gtggactcaa actcgccgtg ggcatcttca gcggtggatg gaatcggcgc
     2941 aaactaaaga ttctccgccg cgattgtgat aggagtcagc ctacaggccg agttgtaacc
     3001 aagcgctgcg tcgttgttgg ttttgaatat aactccatta tcaaaagatt attttaacac
     3061 catgattggc gtcgatgaac ttaacgcgtg catcattggc tgccggaagt gcccgcggct
     3121 ggtgggttgg cgtgaggctt gcgcgaaaaa gccgccccgt cgttacgttg gtgaagacta
     3181 ctgggccaaa ccactagcag gagtcggcga ccccagagcg cgggtgctga tcgtcggact
     3241 ggcgccggcc gcccatggcg gtaatcgcac cggtaggatg tttaccggcg accgtagtgg
     3301 cgactggctt tatggcgcac tttaccaggc tggttttgcc aaccagccca attccgatca
     3361 tcgagacgac ggcttgaagc tgatcgattg ctacgttacc gcggcggtgc actgcgcgcc
     3421 gccggcaaat aaacccaatc tagtcgagtt taaacgttgc cggccttatc tcatcgagga
     3481 gctgcggatt tttagcacgg cccgagtcgt tatcgtgctt ggcaaggtcg ccttcgactc
     3541 tttgcttgcg gcctatcgcg aaaccggcgg ttcgttgtcc aagccgcggc cgaaatttgg
     3601 ccatggcgct tcgtaccgac tcgccaacgg agttcggctc atctgttcct atcatccgag
     3661 ccaacagaac acatttactg gcaagctcac ccgggcgatg tttaaaagcg tcttcgatca
     3721 ggccagggcg gaattgctgg cgtaattttt gatcgtggag agcggacttt acgacgcagt
     3781 cttttttctc ggatcggttc ggtgtgagtc aagtggggtt tgatgcacgc cgacgtgagc
     3841 gctgcgatgc tctacccgca cacgactatg ggtcggatcg attggcaggc gctgatcgca
     3901 gggagcttgc cgctggaagc atctggccct gaccaaggcg cgatgaatcc gtgcaacggt
     3961 taaagtttca ggtttggggt agaggtagat tgcaagacag cgtgggggcg ataatgaaag
     4021 ctggcaggga ttattttgag atcgaagcgg cactaaagaa acgaatcgat ggcgatcttc
     4081 atttcgacca atattcgcgg ctgctctatt ccaccgacgc gtcgatttat cagatcgaac
     4141 ctatcggtgt ggtggtgccg cgccataaag gcgatgttga ggcggcgatc gaagtcgcca
     4201 gacgtttggg cgtttcgatt ctgtcgcgcg gcggcggcac gtcactggct ggacaagccg
     4261 tcggccactc gatcgtattg gatttttcca aatacatgcg caacgtattg gaagtgaatc
     4321 aagaggagct gtggtgccgg gtccaacccg ggctggtgca agacgaactc aatgcttatg
     4381 tgcgcgggca gggattgcag ttcggcccgg acacttcgac gtcgaatcgc gccaccatcg
     4441 gcggcatggt cggcaacaat tcctccggcg ctcattcgct tacctatggc aagactttgg
     4501 atcatgtgat cgagctgacg gtgttgttcg ccgatggttc ggaagtcgtc ttaaaggatt
     4561 tggcgccgga agttgtcgag ggcaagagca aagctaacac catcgagggg catgcctacc
     4621 gggaagtttc tcggctggcg cagcagcaca agagcgaaat cgtcgcgcgc tatccgaaaa
     4681 tcatgcgacg ggtgtcgggg tataatctcg atgagttcgt caagccgcag ccgttcaatc
     4741 tatcgcggat gatcgtcggc tcggagggaa ccttggcggc catcgtcgag gccaagatgc
     4801 ggctcgtggc gaaacccaag tggaccgcca tgaacgtgat ccacttcaac gacgacattg
     4861 atgcgttgga gtgcgcccag atcattttga cgaccaaacc ctacgcgatg gagtcgaccg
     4921 acaagatgat cttgaatctc gctcgcggca acatcgaaca gtcgcgcaaa ctgggctttg
     4981 tccaaggcac gccggattcg cttttgatgg tcgagtatgc cggcgaaacc gaggccgagg
     5041 tgcgggctca agtcgaaacg ctcgaagagt tgcgcaagcg cgagaaaatc ggttacgcgg
     5101 cgactctggc gttcaaagct gaagaagtga agtcgatttg gggtgttcgt aaagcgggac
     5161 taggtttgct gcttggcatg aaaggcgaca agaagcccat cgccttcgtc gaggatacgg
     5221 cggtggcacc ggcgaagctg cccgagttca tcaaacgatt tcgcgagatc gtcacgcgcc
     5281 acgataccgt ggccggttac tacggccatt gttccgtcgg ttgcatgcat attcgcccgt
     5341 taatcaattt gaaagaaaac ggcgaaaagc aaaagatggt gtccatcgcc aacgcgatct
     5401 ccgacttggt gctcgaattc aacggcgcca tgtcgggcga gcatggagac ggcttggcgc
     5461 ggagccactt caacgccaaa ttatttggcc cggcgcttta cgagggcttt cgccagatca
     5521 agcgcgcttt cgatcctaat aatctgttca acccaggtaa aatcgtcgac gccccggcga
     5581 tgaccgagtc gctgaagatc agcccgcagt acaaaacctg ggaaccgcag acgacgttgg
     5641 acttcaccga acaaggcggc tttgcgcgcg ccgtagagat gtgcagcggc atgggcgagt
     5701 gccgcaaaaa gctcgacggc accatgtgtc cctcgtacat gggcaccctc gacgaagaac
     5761 actcgacccg cggtcgcgcc aatgcgttgc gcaacgttct ttccggtttg gtgccgaaag
     5821 ccgagttcac cggcaaacgc ttgtacgacg ttatggacct ctgcctcgaa tgcaaagcct
     5881 gcaaggctga gtgcccatcc aacgtcgaca tggcgaaatt gaaatatgag ttcctcgacc
     5941 actatcaccg cgccaacggt ctgccgctgc gcaatcgcat cttcggtggc atagccagcc
     6001 tcaatcgcat tggctcggcc ctggctccgg tctccaattg gcttgccaac tcgctaccga
     6061 accggtggtt gatggatacc ctcgccggca tcgatcggcg ccggccgttg ccgcagttcg
     6121 ccggtgagag cttcgaagat tggtttcgag ttcacaagcc cgaaggcgac ggctcaaagg
     6181 gcgatgtggt tttgtttcac gacaccttca ataattacaa cacgcctaac gtcgccatcg
     6241 ccgcgacgcg tttcttggaa aaatccagtt atcgagtact tctcgtcgac aaaaaatgct
     6301 gcggccggcc gatgatttcc aaagggatgc ttggccaagc gaaggcgcat gctgcttgga
     6361 acatcgcaca gctcgcaccc tatgcgcaga agggcacggc gatcgtcggt ttggagccgt
     6421 cctgtctatt gacgctgcgc gatgaatatc ccgagttcat ccgcaccgat gcggctaaga
     6481 aagtcgccca gcatagtttt ttgctcgaag agttcgtcat gcgcgaagtc aacgccggca
     6541 gactctcact gggtgcccac ggcaaaggac aaaaagttct actgcacagc cattgtcacc
     6601 aaaaagcctt ggtcggcagc gccccgacga tcgccatgct caaagccgcc ggctacgaag
     6661 tcagcgaagc cgactccggt tgctgcggca tggcgggctc cttcggtttt gaaaaagaac
     6721 actacgatct gtccgtcaag atcggcaatc gccgcttggc gccggccgtc aaagcggcgt
     6781 cgattgacat cgaagtggtc gcaccgggaa tttcctgccg acagcagatt gatcatctgg
     6841 cggctaggaa ggcaaagcat ccagccgagt tgctggtgcc gcatcaataa cgcttcgatt
     6901 ggacggtggc cctatcgcca atggtcgagt ggcttcagag tgacttgtgt cgggatgcgg
     6961 atttgccggc tataattgca gagtaaagcc tttggtcgaa tctaaccggc ttgtttacca
     7021 aagaagtcgc tgcaacaaag tttcatagca caccccgtcc ggcaaacgtg tcgcattgct
     7081 tcaagtcgcc ggtttcgatt cctttgcgaa accagcgcat gcgctgttcg gcgctgccgt
     7141 gggtgaaacc ttcgggtacg acatagccct gggagcgttt ctgcaatgtg tcatcgccaa
     7201 tttgcgccgc ggcattgagg ccttcttcga tatcgcctgg ttcgaggatc ttgcggtcgc
     7261 ggttggcaaa gctcgcccaa atgccggcta aacaatccgc ttgcagctcg agagcgaccg
     7321 atagcgcatt ggcttcgcgt tcgcttgcac gctgctggcg cgcttgcact ttgcccatga
     7381 tgccaagcat attctgcaca tggtggccga cttcgtgagc gacgacataa gcctgggcaa
     7441 aatcccctgg ggctttgaaa cggtcgcgca attcacgata gaacgataaa tcaatgtaga
     7501 ccttttggtc gttgggacaa taaaacggcc caaccgcggc ttgagcaaaa ccgcaagcgg
     7561 actgaacctc gcccgagaac aggaccaatt tgggttgcgc gtattcacgt cccgctttct
     7621 gaaatatctc gccccacacg cgttcgttac tgccgagcac ccgcgagaca aactcacgcc
     7681 cctcatcacg gccggtttgc ggtgcttgtg ggatcggctc gttggaaacg ttcgattgcg
     7741 gatcgccctg aagaatcgcg ctgggatcga tgccgaaaaa catcgcgatc agcacgagca
     7801 caacggtccc cagcccaccg cccactgcgc ctcgtgtgac gcgggaacct cgccgatctt
     7861 caacgttttg actttctggt tcgttgtcga gccgcatata acctccttgc gtggtgccgt
     7921 aatgaatggc ggagcgttgg ctcttgaata tggcttagta ccaaaaaaaa caaccccgcc
     7981 gcgttgccgc gagcggggtc gttgacattc agagttaagt taacggttat tttttcgctt
     8041 tgctatcgtc cattggctta gcaggggtcg tgctcgtgga cgtggacttc tcgtccttca
     8101 tggctccggc cttggtatcc ttggcggccg gctcggcttt tttaggaacg acggtatttc
     8161 cagttaccgt tacattagcc cggatctgct tcatcgcgcc gggtccgatg cccttgactt
     8221 tttccagatc gtcgacgctt ttgaagtcgc cgttcttctt gcgatagtcg atgatgtcct
     8281 gcgcctttga gtcgccgatc cccttcagag cttggagctc ttctttagac gcggtgttga
     8341 tattgaccat tagaccgccg gtcttggctg ccgtcgcgct tttggtatcg gtgctatctt
     8401 tggcaggggc tttagtgtcg ctcgctccct tggtcgctgc cggtgctttc ttttcctcca
     8461 tcgccttgtc tgccgcagtc gccatagccg agatacccgc cgccgcaaac cacaatactc
     8521 ccagtaacac tagttttttc atttgtcctc ctagtttttt acagccgatc cgttgaacta
     8581 accattagac gctgcttgga caatttgatt catcgcctcc gtctgccgga cggctggcct
     8641 tgatgggacg gggtattatc gagactggtg gctcatgtca agaaaattca tttgctgtgc
     8701 tccagcgccg acttagccga gcgcgatctc gggcaggtcg gcggaccatc ccggttgatc
     8761 cggtataaac aaccagaatg gtttgccgtc ctcctgccag ctggctgaca cgccttccaa
     8821 cagttcgagc gccaggtcaa aatcggcttg gtatttcacc acgaaaggtt tgcaattggt
     8881 taagattaac acccaaccgt tgccgtcgag ccaagcgaga tcggtcaagc aatcgttgag
     8941 cgcgtcccag ttcttgccga agtgcttggg gaatttgaga gttctcgaaa acgcgtcgaa
     9001 cagaccgctt ttgccacgcg ccttggcaag gtcgatttta aacacttgca attcggcggt
     9061 cttggcggcg gcgacgatcg cgtccgagtc tatggccgtc cgatagacgc cggcgcgttt
     9121 gctgtttggc agaagattga gcagggagcc catgggttca ttccaaaatg cgtcgaaagg
     9181 tttgatagtg atcgtcggtg aaatagaatt cgcctttgcg tccggcgacg atccggcgcg
     9241 cgccccggtc gcgcgcgccg ggtgtcggta cggtgtattc tttgtagtag ccgcgctcct
     9301 tttgcggcag ccggcgctcg aaattgccga aagtcgcgcc gtcccgatgg tatgggaatg
     9361 gcccgtcctt ctttatcagg ctcagggttt tgtgcgcttc tcggggtaac tcggtgagga
     9421 ggacttggtc ggcttggcga tcgctcggtt cacgcgcaca acttgtcccg ccgccaacaa
     9481 ggagaaccaa cgcaagtgcg agaacataaa cagtcggtgc ccgttgggag caccggtaga
     9541 ttggcaagcg cgtcacgggc gtaggctagg gtttcgctgt aaatccaggc ttttatattc
     9601 tccgcatgat caatttggcg atcagtccga cgacaaatcc gtgccgacga gtcccgacgt
     9661 tgatttctcg cacaagcgct atgtctacgg cgtataaccg ccagcgcctt cactgtcaat
     9721 gtttgctgtg tggactgcat cgccagtcgc tgtatttaat gttgccaatg ctcgatggat
     9781 gccacgagcg gcgggtttac cccggtaatc aagcaagctg attagtttat ttgtgcgctg
     9841 ggctgaggta agcattgttg agtattcgct gccatgcggc gcgggaggac gatggagcaa
     9901 gcgagaacaa tagtgcgcct gtgggccgac cgtcatcgac aggcggtcat gtatgacgat
     9961 gacagtgcgg ttctattaga cgtcgcctcg ggcaagcggc tgaagatcgc ttggccggcg
    10021 ctggtcagct acgaagagaa agtacatcct gaaacccagg agccttacat tgtcttgctc
    10081 ttcgacgatg gcacgcaact ggcgttggtc gaccctggtg gcgtcgcctt tgcgccgttg
    10141 acggtgaaca ccggtgcgct ggagaatctg ccggcggtgg tatgtttgcg cgatctgctc
    10201 ctgctcaagc aacgggtcga ccattttctc tacggccatg ccgacgagcc gccgccgcgg
    10261 gaatgtttaa atctcatcat gatttgcat
//
LOCUS       DASQKS010000179         6838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2631902, whole genome shotgun sequence.
ACCESSION   DASQKS010000179 DASQKS010000000
VERSION     DASQKS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631902"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VE170_04525"
     CDS             <1..665
                     /locus_tag="VE170_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HYJ14729.1"
                     /translation="RMGVGQHFERPPDDYLREMLDSDDILLKGITLERLQREDAVLLN
                     RPAAPYVGFADLRFKTPSGRIEIYKEELHRHGAALPYYHEPIEASPKNPIYAEYPLTL
                     LFSHSRHRIHSTFANLSRAKQHEPEPLVEIAPDDAARRCLSDGQLVRVYNPRGCVELK
                     CRLNADLRAGVVVIAEGSWVKDFPKGEPYSLTHEQVSPTSDNYAFFDTLVEITAAGKN
                     TD"
     gene            complement(662..1270)
                     /locus_tag="VE170_04530"
     CDS             complement(662..1270)
                     /locus_tag="VE170_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HYJ14730.1"
                     /translation="MAKFFVLLFIAVVGEWLFQGSPLLPAESLNKEFPLLKINKALDK
                     RTVIKPSMIPNGGNGLYALVPIKKGEVIGELGGRLLPADKYPQGNHYIAALLECAQKH
                     AHPYVYLDAKDNGANVSRINFAPSKINGVDTHLQNAAIRRLCQPPYFEFYALRDIPAG
                     EEIFSSYGPNYDYDRFMHQPDVKEFFCKRAKIDCRAKYSFDH"
     gene            complement(1335..2132)
                     /locus_tag="VE170_04535"
     CDS             complement(1335..2132)
                     /locus_tag="VE170_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14731.1"
                     /translation="MEVIDIHHHLGALNGGSLQEGDDWPDRDYVNRVRIMDANGVNAA
                     AILAATGYIQADGIKDTMRSNDVVAAYRLRDPKRFPIACGIVEPLHGARSLGELERIK
                     SELGLEGVVWHSRFQGVAVDHQLMRPLLKKVQELGLVPLIHTNAESNLEAVWRLERLA
                     LEFPDLTFISLDALTTNTNSQLALDIAKRTQNIIFDTAHVRGPGYVKSFVEAVGSERM
                     VFGSLFYSHPASYDHCVSLAEIKAAKISDADKANILAGNVKRIFQLK"
     gene            complement(2150..2782)
                     /locus_tag="VE170_04540"
     CDS             complement(2150..2782)
                     /locus_tag="VE170_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HYJ14732.1"
                     /translation="MKVLMPMNLKRLVASLCLLNLTWTLGLTAVSADEEQPPRLVPIL
                     KIDEGLDKRTAIKPSLIPGGGNGLYAAAAIKQGDVIGMLGGQLRTDEDYPAGNYYLAS
                     IPECAWEETKPYKYLDAKHFGAHVSRINFAPKTINGIEAGLQNAALRQLCNYPYVIFV
                     ALRDIDVGTEIWSSYGPYYPYDQFMYEPAVREFFCNQAKIDCRENYSFEP"
     gene            2901..3974
                     /locus_tag="VE170_04545"
     CDS             2901..3974
                     /locus_tag="VE170_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14733.1"
                     /translation="MPTIDADAHVVESERTWDYMDKADEKYRPVIVKPRGSGSAEYWF
                     IDGKIRGLVRVIMTAKEMDDVAQRTGRVMGTPQETREMENVPARLKHMDELGIDVQVL
                     YPTVFIEQISEKPAWEIAICRGYNRWLADIHQQGQGRLRWICVLPLLDMGAAVEELKF
                     CQQHGAVGVFMRGIEGSRMITDPYFYPLYDAMSRLDMAVGVHVGNGSPHNIDMVSQYN
                     GGGSFWKFRIPVIGAFHSVIMGEIPQQFPKLRFHWAEAAANWVPYVVKDLQRRWATQK
                     KDLPDNPLKEYRQYVSCQTDDDVDYVVKYAGEDNLVIGTDYGHNDQSTEVEALRNLKK
                     TGMITDGQYDKICYHNPKALFAL"
     gene            complement(4419..4673)
                     /locus_tag="VE170_04550"
     CDS             complement(4419..4673)
                     /locus_tag="VE170_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14734.1"
                     /translation="MGIIRLRLVYRLLSKTNRIAMKQPNTSINDGQNWQWPDKTWFRQ
                     RLNAIKAEENQQRRRPMVNSSQPAKATSPETDSSDRLKAE"
     gene            complement(4821..6014)
                     /locus_tag="VE170_04555"
     CDS             complement(4821..6014)
                     /locus_tag="VE170_04555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ14735.1"
                     /translation="MPPLSEFVLLALCLLWICGIVYLSLAQFRLAQFHRLRFSPPQEW
                     PVLSVIVPACNEAANIESAVATLIRQDYPNFELILVDDRSTDGTGAIIDRLAASDQRI
                     HALHVEVLPDGWLGKVHALERAVEVAKGDWLLFTDADVHFAAGTLRRAVGWAVDRQVD
                     HLALAPLALQSSFLLDIVVHTFMLLFLIGTRAAGLNRPGSRGFAGVGAFNLIRRAAFQ
                     RTPGFSWLRLEPCDDVGLGLMIKRAGGRSFFAFAQHHLSVTWYPSVPAMFAGLEKNLF
                     GATANYRWWMTIVQVTGIYALAAAPVLAVILGLVEGRHLLFSAGLAVLLTHWAFSLAA
                     ATEGWRRRLSLMLVPIGLGMIGAMMARAAWKCLAHGGIAWRGTHYPLDQLRAGQRVKL
                     ASRQF"
     gene            complement(6049..6678)
                     /locus_tag="VE170_04560"
     CDS             complement(6049..6678)
                     /locus_tag="VE170_04560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HYJ14736.1"
                     /translation="MRLILASTSPRRREILSLLGLSFDAIAPDFDEAVSAHLSIEEEV
                     LDFAVGKARSVAVHHPDAIVIGSDTMIFLGGAKIGKPLDLADAKRILRLLSGKTHLIY
                     TSLAIIDGCGGDGIQMVAKVSVDMRPYDDNAIEDYLAYDESMDKAGAYSIQGQGSKLI
                     AAIDGDFLAAVGMPLRPIADYLRRRRVELPIDIDELHAKKDFLNWRQFA"
BASE COUNT         1543 a         1962 c         1960 g         1373 t
ORIGIN      
        1 agcggatggg ggtggggcaa cacttcgagc gcccaccgga cgattatttg cgcgagatgc
       61 tcgacagtga tgatattttg ctcaaaggca ttactctcga acgcttgcag cgcgaagatg
      121 cggtcttgct caaccggccg gccgcgccct atgtcggctt cgccgatctg cggtttaaaa
      181 ctccgtcggg gcgcattgaa atctataagg aagaactcca tagacacggc gcggctctgc
      241 cctattatca cgagcccatc gaggcgagtc ccaagaatcc aatctatgcg gagtatccgc
      301 tgacgttact gttctcccac agccgtcatc ggattcattc gaccttcgcc aatctgtccc
      361 gagcaaagca gcacgaaccc gagcctctgg tcgagattgc tccggatgac gcggcgcgcc
      421 ggtgcctgag cgatggccag ctcgtgcgag tttacaaccc gcgtggatgc gtcgagctaa
      481 agtgccgcct caatgccgac cttcgtgccg gcgtggtggt gatcgcggaa ggctcctggg
      541 tgaaagattt tcccaaaggc gagccctata gcctgaccca cgaacaggtc agcccgactt
      601 cggacaacta tgcttttttt gacaccctgg tcgaaattac cgcggctggt aagaacaccg
      661 actaatggtc gaaggaatac ttcgcccggc agtcgatctt ggcgcgcttg cagaagaact
      721 ctttgacgtc aggctggtgc atgaaccggt catagtcgta attgggcccg tagctggaga
      781 atatttcttc accggcgggg atatcccgca gggcgtagaa ttcgaaatag ggcggctgac
      841 agagtcgtct aattgctgcg ttctgcaaat gcgtatcaac gccgttgatt ttgcttggcg
      901 caaagttgat ccggctgaca ttggcgccgt tatccttggc gtctaagtaa acgtagggat
      961 gggcatgctt ttgcgcgcac tccaacagcg cggcgatgta atggttgcct tgaggatatt
     1021 tatccgccgg cagcaatcgt ccgccgagct cgcctatcac ctcgcctttc ttgatcggca
     1081 ccagcgcata taagccgttg ccgccattgg gaatcatcga tggcttgatc accgtgcgtt
     1141 tgtcgagcgc cttgttgatt tttagcagcg ggaattcctt gttaagactc tccgccggca
     1201 acaatggcga accttggaat aaccactcgc ccacgacggc gataaataac aggacgaaga
     1261 atttagccat acagaagcga atcgcaaccg gctcctctca aaatcgaact ttgccgcgag
     1321 caaattcgcc gcggctattt gagctgaaaa attcttttga cgttgccggc caaaatgttg
     1381 gccttatcgg cgtcgctaat cttggctgcc ttgatctccg ccaagctcac gcaatggtcg
     1441 tagctcgccg gatgggaata aaacaggctg ccgaaaacca tgcgctccga gcccaccgct
     1501 tcgacaaaac ttttcacata gcccggcccg cgcacatggg cggtatcgaa aataatattt
     1561 tgggtccgct tggcgatgtc caacgctaat tggctgttgg tgttggtggt caacgcgtcc
     1621 aacgagatga aggtcagatc gggaaactcc agcgcgagcc gttccaagcg ccacaccgct
     1681 tcgaggttgg attcggcatt ggtgtgaatc agcggtacca ggccgagctc ctggaccttt
     1741 ttgagcagcg gccgcatcag ctggtgatcc accgcgacgc cttggaagcg gctatgccag
     1801 accacgccct cgagaccgag ctcgcttttt atccgttcca actcgcccag cgagcgggcg
     1861 ccgtggagcg gctcgacgat gccgcaggcg atgggaaaac gtttgggatc gcgtagtcga
     1921 taggcggcga cgacatcgtt ggagcgcatc gtgtccttga tgccgtccgc ctggatatat
     1981 cccgtcgccg ccagaatcgc cgcggcgttt acgccattgg cgtccatgat gcgcacgcga
     2041 ttgacatagt cacgatccgg ccagtcgtcg ccttcctgca agctgccgcc gttgagcgcg
     2101 cccaagtgat gatgaatatc gataacttcc atagcatctc tccagccgtt tagggctcga
     2161 aactatagtt ttcccggcag tcgatcttcg cctgattgca gaagaactct cttaccgccg
     2221 gctcatacat gaactgatcg tagggataat aggggccgta gctggaccag atctcggtgc
     2281 ccacgtcaat atcgcgcagc gcgacgaata tcacataggg ataattgcac agctggcgca
     2341 acgcggcatt ttgcaaaccc gcctcgatgc cattgatggt tttgggcgcg aaattgatcc
     2401 ggctgacatg ggcgccgaaa tgtttcgcat cgagatactt atacggcttg gtctcctccc
     2461 aggcgcattc cgggatggat gccagataat aattgcccgc cggataatcc tcatccgtgc
     2521 gcagctgacc gccgagcatg ccgatgacat cgccctgctt gatcgccgcc gcggcgtaca
     2581 gcccgttacc gccgccggga attaacgacg gcttgatcgc cgtgcgctta tcgagcccct
     2641 cgtcgatttt aagaatcggt accagccggg gcggttgctc ttcgtcagcg ctaacggcgg
     2701 taagcccaag agtccaggtc aaattcagca agcacaggct cgcgaccagc cgtttcaggt
     2761 tcatcggcat caagactttc actttctgaa cactctacga atcatgcatc cacgacgatg
     2821 tcaaataatc cccactctgg cgttgacagt ggcgccaacg gcgcgctaga agcgggcaac
     2881 agccgacgga ggtgacagtc atgccgacta tagatgccga cgcccacgtg gtcgaaagcg
     2941 aacgcacctg ggactatatg gataaggccg acgagaagta ccggccggtg atcgtcaagc
     3001 cgcgcggcag cggcagcgcc gagtattggt tcatcgatgg caaaatccgc ggcctggtac
     3061 gggttatcat gacagccaag gaaatggacg acgtggctca acgcaccggc cgggtcatgg
     3121 gcacaccgca ggaaactcgc gagatggaaa atgtcccggc gcggctcaaa catatggacg
     3181 agctcggcat cgacgtccaa gtgctctatc ccaccgtgtt catcgaacag atctccgaga
     3241 agcctgcctg ggagatcgcc atctgccgcg gctataaccg ctggctcgcc gacattcacc
     3301 aacaaggcca ggggcgcttg cgctggatct gcgtcctgcc gctgctcgat atgggcgcgg
     3361 cggtggaaga actcaagttt tgccaacaac atggcgccgt cggcgttttc atgcgcggca
     3421 tcgaaggcag ccggatgatc accgatccgt atttctatcc gctctacgat gccatgagcc
     3481 ggctcgacat ggcggtcggc gtgcacgttg gcaatggcag cccgcataac atcgatatgg
     3541 tgtcgcagta caacggcggc ggcagcttct ggaaatttcg catcccggtt atcggcgctt
     3601 ttcactcggt catcatgggc gaaatcccgc agcagtttcc caaactgcgc tttcattggg
     3661 ccgaggcggc ggccaactgg gtgccctacg tggtcaaaga cttgcagcgc cgctgggcaa
     3721 cgcagaaaaa agacttaccc gataacccgc tcaaagagta ccgccagtat gtctcctgcc
     3781 agaccgacga cgatgtcgac tacgtggtca aatacgctgg cgaagacaat ctcgtcatcg
     3841 gtaccgacta cggtcacaac gaccagtcca ccgaagtgga agcgctgcgc aacctaaaga
     3901 agaccggcat gatcaccgat ggtcaatacg acaagatctg ctatcacaat cccaaggccc
     3961 tgttcgctct gtaactcggt caaatatcga ggattccaag tttcctcgca atgctgccat
     4021 ccgctgcttt ccggccatgg acttttgaac taacgcagtt aaagatccgg cgtctcaaag
     4081 aatttttgca acggctagcg atgatcgcta atggtttgcc gaacacaatg ccagcccggt
     4141 tcattcaacc cgatcaacta cagccaggga atcctcgaag taacggcgga caaagagagt
     4201 aacagcagga attgaaaaag gcagcgttgc gatcgacaag cgctcgatat aaccgccaag
     4261 attttagcgg ataagaatgc ggacgtagaa gggaaaggac cgaagactac gagaatgatt
     4321 gctgatgctg gggcttcggt gaatctgtgc ggtcgcgggc cgaggtattt attcccgccg
     4381 gctcggaagg cgtgcggtcg caggcgaagg caaaccgctt attcggcctt caagcggtcg
     4441 ctggagtcgg tttccgggga agtagccttg gctggttgac tggagttcac catggggcgg
     4501 cgtcgctgct gattttcctc ggccttgatg gcgttcaacc gttgacgaaa ccaagtcttg
     4561 tccggccact gccagttttg cccgtcgttt atgctagtgt ttggctgttt catggcaatc
     4621 ctgttcgtct tgctgagcaa tcgatagacc agccgtaagc gaataattcc cacgaagttt
     4681 tgggagcatt gccgcctgta gggtgacgag tcttcaccgg cgggagtgcg aatttggcgt
     4741 tgagaatcgc taacgaatta caattttgcg caagaatttg atccttcaac ggctgaaaac
     4801 aactgcaccc gcgaggccgg ctaaaattgc cggctggcga gcttaacccg ttggccagcg
     4861 cgcaattgat cgagcggata atgggtgcca cgccaagcga tacccccatg ggcaaggcac
     4921 ttccaagcgg cacgagccat catggcgccg atcatgccaa gtccgatcgg cacaagcatc
     4981 aaacttagcc gcctgcgcca gccttcggta gcggccgcta ggctaaatgc ccaatgggtc
     5041 aatagcacgg ccaatcccgc gctgaacaat aggtggcggc cttcaaccag gccgaggata
     5101 acagcaagga cgggggccgc cgccaaggca taaatcccgg tcacctgaac gatggtcatc
     5161 caccaacggt aattggccgt tgcgccgaag agatttttct ccagccctgc gaacatcgcc
     5221 ggcaccgacg ggtaccaggt cacggacaga tggtgctgcg cgaaggcgaa aaacgatcgc
     5281 ccaccggcac gctttatcat caagcccaaa ccgacatcat cgcagggttc gagacgcagc
     5341 caggaaaaac ccggcgtgcg ctggaatgcc gcgcggcgta tgagattgaa agcaccgacc
     5401 ccggcaaagc cacggctgcc gggccggttg agaccggcgg cgcgggtgcc gatcagaaac
     5461 agcaacataa aggtatgcac gacgatgtcg agcaagaaac tcgattgcag agctagcggc
     5521 gccagcgcca gatggtcgac ctgccggtcg actgcccagc ccacggcgcg gcgcagggtg
     5581 ccggcggcaa aatggacgtc ggcgtcggtg aaaagcagcc aatcgccctt ggcaacttcg
     5641 acggcgcgct cgagggcgtg tactttgccc aaccagccgt ccggcaaaac ctccacatgc
     5701 agggcgtgaa tccgctggtc gcttgcggcc aaacgatcga tgatcgcccc tgtgccgtcg
     5761 gtggaacggt cgtcgaccag gatgagttcg aagttgggat agtcttgccg gatcaaggtt
     5821 gccaccgcgc tctcgatgtt ggcggcttcg ttacaggccg gaacaatcac gctgagcacc
     5881 ggccactctt gtggtggaga aaaccgcagc cggtgaaatt gtgccaagcg aaactgcgcc
     5941 aaggacaagt agacaatgcc gcaaatccac aggaggcaaa gcgcgagtaa aacaaactcc
     6001 gacaatggcg gcatggctcg attaaccggt tacatccagt ttgggaaatc aagcgaactg
     6061 gcgccagttg agaaaatctt tcttagcgtg aagttcatcg atatcgatcg gcaactcgac
     6121 acgacgacgg cgcaaatagt cggcgatggg gcgcagcggc atccccactg cggcgaggaa
     6181 gtcgccatcg atcgctgcga taagcttgct cccctgcccc tggatcgaat aagcaccggc
     6241 tttgtccatc gattcgtcgt aggccaaata atcttcgatg gcgttgtcgt cgtaagggcg
     6301 catgtcgacc gacaccttag ccaccatttg tataccgtcg ccgccacaac cgtcgatgat
     6361 cgctaggctg gtgtaaatca ggtgggtctt gcccgatagc aaacgcagga ttcgcttggc
     6421 gtcagccaga tccagcggct tgccgatctt ggcgccgccg agaaaaatca tcgtatcgct
     6481 gccgatgacg atggcgtcgg gatgatgcac ggcgacggag cgcgccttgc caacggcaaa
     6541 gtcgagaact tcttcttcga tactcaagtg cgccgagacc gcctcgtcga agtcgggggc
     6601 gatggcgtcg aaggacagcc ccagcaacga cagaatctcc cggcggcggg gggaagtgga
     6661 ggcgagaatc aagcgcatca attgttaacg ggagagtaca gcgcagatga tagcgcttga
     6721 gccgattccc gcgcgccaac cggcgtatcg gcctgtgcat cgcgccaaat ctggtaatcg
     6781 cgcagacatt ggagcacgtc cgcggtgccg ttgacgcgca ccattttcgt gcgcaact
//
LOCUS       DASQKS010000180        10142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2199298, whole genome shotgun sequence.
ACCESSION   DASQKS010000180 DASQKS010000000
VERSION     DASQKS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199298"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..568
                     /locus_tag="VE170_04565"
     CDS             62..568
                     /locus_tag="VE170_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYJ14737.1"
                     /translation="MEAKETYASLEKLFPGKVGDFKGDVFEPYCHVDKDAIVEICHFL
                     RTAQSFRFEVLSDLTAIDWPKEEKIQMVYHLFSYAHNHQIILKVDLPRDNPKIATLEE
                     QWKVANWFEREVYDLFGVIFEGHTDLRRIMLPEDWSGFPLRKDYIEQEEYDGISTQRA
                     PLVEKLLR"
     gene            578..1717
                     /locus_tag="VE170_04570"
     CDS             578..1717
                     /locus_tag="VE170_04570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632687.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYJ14738.1"
                     /translation="MSETAATDPGFYAEEMTISVGPQHPSTHGVLRFVVKTDGEMINE
                     AIPDVGYLHRSIEKIGEKVTYHGYVPYTDRADYLAAMFANQAFCMAAEKLGNTEVSRR
                     GEFCRVIACELNRLASHLISVGTFGQDIGAMTPFVHALRERETINNLMEEICGARLTY
                     NYIRIGGVGYDIVPSTMTKIAEFLDHFEPIVEEFNRLLSGNKIFIERLAHVAVISKAD
                     ALSYNLVGPNLRGSGIKWDIRRDIPYSVYPELDFEIPVGTAEVGTLGDSFDRFWVRIR
                     EMQESVKILRQAMRMIPKGPAISKVPKKFRPPAGEYYARVEAPRGDMGFYVISDGSEY
                     PYRVRIRTGSFTAMAIIDKLAKGIMMADLIALIGSLDVDAPEIDR"
     gene            1736..2926
                     /gene="nuoH"
                     /locus_tag="VE170_04575"
     CDS             1736..2926
                     /gene="nuoH"
                     /locus_tag="VE170_04575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HYJ14739.1"
                     /translation="MQAFLDQLIQDGAFAGLPKEVVYAIGMVLAAVVVLSVFVAPLAG
                     VTSWLERRVWARMQSRVGPNRVGPQGILQWLADGIKNIMKEDIIPAAADQKLFPLAPY
                     IVFMGFLCAFVVIPFGETLIVADLNIGILYILAVTSLVVVGILMAGWASNNKWSLLGG
                     MRSAAQIVSYEIPAGLSVLTIVFLAGTLSMQGIIKAQGWGPWDWFIFHNPFTFCAFFL
                     FFTAALAEGNRTPFDIPEAESELVAGYVTEYSGMRFLFFFFAEWGNLYVIGAVATTLF
                     MGGWQVPAFFDGYPVLKGMAQFATFFLKAYFWVFVAMWIRATLPRVRVDQLMGLCWKY
                     MVPLSFVCLLGTIGFMFIDEISRRVFGAITLGFAVATIINFFRRVGFQFRAAKPEAYW
                     KPHI"
     gene            2940..3506
                     /locus_tag="VE170_04580"
     CDS             2940..3506
                     /locus_tag="VE170_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HYJ14740.1"
                     /translation="MGVLTSYVKDIQAAIASTFEGMSITFSHLMRKPMTIQYPDKIPV
                     PIQETLPLRYRGILEVDLDICTGCLACERICPITCITIGIDMNKETKQRQFTHFDIDV
                     CKCMYCGLCSEVCPTGAIRHSQEFEGASYNPAGLVRHFAPEPSNLYKPKKGGETEPKL
                     ITKVDIGLKYLDEFATPDGGGAAEEEAK"
     gene            3626..4126
                     /locus_tag="VE170_04585"
     CDS             3626..4126
                     /locus_tag="VE170_04585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYJ14741.1"
                     /translation="MTVSTAVFYLIALLTIVSAGMVAFSRNIIYSAFSLLGTFMGIAG
                     LYIFLGADFVAAVHVLIYVGGILVLILFAVMLTHRITDVTITNRAAGTIPALIVIGVL
                     VYFLIQTVWTTPWVKAKEVVFTATTAKIGDAFLYDYLLPFELASLVLLAAMIGAVVLS
                     RKEIKD"
     gene            4129..4440
                     /gene="nuoK"
                     /locus_tag="VE170_04590"
     CDS             4129..4440
                     /gene="nuoK"
                     /locus_tag="VE170_04590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYJ14742.1"
                     /translation="MSPNELSSYLTIGAILFALGLFTVLTRRNAISVLMGVELILNSA
                     NVNYVAFSHFSSGNSAGQIYAIFVIMLAAAEAAVGLAIVLAIFKLFHTIDVEAAETMR
                     D"
     gene            4482..6524
                     /gene="nuoL"
                     /locus_tag="VE170_04595"
     CDS             4482..6524
                     /gene="nuoL"
                     /locus_tag="VE170_04595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYJ14743.1"
                     /translation="MENPIQTDFIRWIVFLPLLGAMVNGLLGAKIQKSLGKGAIGVIA
                     CAPVIVAFALAVYAFFILKGLAPEQRFLIDNLYRWIDLGSLRVDMAFLVDPLSSVMIL
                     VVTGIGGLIHIYATGYMHDDKAFWRFFAYLNLFTAAMLTLVLGDSLLLLFVGWEGVGL
                     CSYALIGFWYHDHANARAGNKAFIVNRVGDFGFVLGMFLLFWSLDAQGHGSLTIREMA
                     KWAPSIKDTLIWGWPVVTVATLFLFVGATGKSAQIPLFVWLPDAMAGPTPVSALIHAA
                     TMVTAGVYMTARMNVFFSMAPATLHLIAWIGVATAIVAATIALTQNDIKKVLAYSTVS
                     QLGYMFIGVGVGGYAAAVFHLMTHAFFKACLFLGAGSVIHAMHHEQDMRKMGGLKKWM
                     PITFATFFISVLAIAGFPPFAGFFSKDEILWLAFTSEHGNKAVWFLALCGAGMTAFYM
                     FRQLFMTFYGECRADHHTQEHLHESPPVMTMPLVLLAIGAVFAGLIGLPGVLGGSQFA
                     HWLEPVIHAHGESHASHALEWGLMAVSVAVASFGVFIAFLMYRRQSLSPDTFVNLAGG
                     LPYRLFDRKWYFDEIYQAVFVNGTLLLARFWSAFDAYIVDGIVNGVATLTRFVAWFNG
                     LFDTYIIDGLVNGLSNVTYWLGNRFRRLQTGNINGYLYGIIIAMAVAVFVKVRYWS"
     gene            6547..8064
                     /locus_tag="VE170_04600"
     CDS             6547..8064
                     /locus_tag="VE170_04600"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYJ14744.1"
                     /translation="MQNILTYMTFIPLAGAAVVLALPNNAKLVRWFSAIVTVPPLLMA
                     IWLFMNFDRTTDNFQFVEDYHWIRAYNISYTMGVDGLSITMVLLTALLSFLCIFASFG
                     IEKAVKGYFALFLMLDAGMMGVFCALDFFLFYVFWEVMLLPMYFLIGIWGGPRREYAA
                     IKFFLYTLFGSVLMLLAILALYFTVEPHTFDMRIMMAQASKYSTAVLPMWPFNGWGWS
                     FQHAIWLALFIGFAIKIPAFPFHTWLPDAHVEAPTAISVILAGVLLKMGTYGILRVNF
                     PMLPVATADLAYWFLGALGAWNIIYGAFCAMAQKDLKKLVAYSSVSHMGYVMLGMASF
                     TTQGLNGAVLQMFNHGTITGMLFLLVGVVYDRAHHREIDGFGGLAAIMPIYTGITALA
                     FFASMGLPGLSGFISEILVLLGTWQKYPVLTILGASGVILTAGYLLWTIQRVYLGPPN
                     EKYLTMAEINGREIFTLVPLGIIVIIVGVYPGVVLDMLRTTLDSLNAIVIPHLHG"
     gene            8068..9552
                     /locus_tag="VE170_04605"
     CDS             8068..9552
                     /locus_tag="VE170_04605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYJ14745.1"
                     /translation="MDLKNVASLTYFCPEIVLSVTILLIILLDLLAQSRRWPAAVALV
                     GCLAALVVSFDLYSAQPGLLFHRMIVLDNFAIFFKVFSLAAAILCILMSMGSKEIDRV
                     HGAEYYALLLTCTLGMFFMASSSNLLMAYLSLEMVSLGSYVLTGFLPHSRRSSEAALK
                     YLIYGGVASGTMIYGMSWIFGMTGSLDYGAIQVALSQEGLHKVALFMAFIFILAGFGY
                     KIVFFPFHMWSPDVYQGAPTPFTAFLSVASNAAGLAIMIRFFFPGVSKLLPSGDWAFT
                     AGVEWPQVLLALSMLTMTIGNLCALNQRNVKRMLAYSGIAHAGYMMMGLAILNNEGLA
                     AILFYAVVYLIMNVGAFLVVGMIANVTGDEDIDDYRGLAWRGATVPAICLAIFLFSLT
                     GLPPFAGFIGKFFLFSAVIKQGGAFAILAIVAALNSVISLYYYAKIVKIMFLDLPLPE
                     DKQISLARNDFALLLPLTGLTILLGVYFGPLVQYTNHALQFYTK"
     gene            complement(9616..>10142)
                     /locus_tag="VE170_04610"
     CDS             complement(9616..>10142)
                     /locus_tag="VE170_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ14746.1"
                     /translation="LFALWHGRMYLPIDAHRHQSIVTMASKSLDGDIVARWLENNGYL
                     VTRGSTSRGGSEALRRMVRQVRLGHNVALTVDGPRGPVHVVQAGVVRLARITGAWILP
                     ITFSSSWPLFLRSWDRYLLPKPFSKNVVAYGEPFAVSKLMSDEAALEKIRASLDEVTA
                     AADKIAGIIPPSRA"
BASE COUNT         1884 a         2887 c         2858 g         2513 t
ORIGIN      
        1 acgctggctg gtaagggcgc cagaggtcga gaacgccgaa gccggggctt aagactcacc
       61 catggaagcc aaagaaacct acgcctcatt ggaaaaactc tttcccggca aagtcggcga
      121 cttcaagggt gatgtcttcg agccctattg ccacgtcgat aaggacgcca tcgttgaaat
      181 ctgccacttc ctgaggacgg cgcagagttt cagattcgaa gtgctttcgg atctgaccgc
      241 catcgactgg cccaaggaag agaagattca gatggtttac catctatttt cttacgcgca
      301 taaccatcag attattctca aagtcgatct gccgcgcgac aatccgaaaa tcgccacatt
      361 ggaagagcag tggaaagtcg ccaattggtt cgaacgcgag gtttacgatc tcttcggggt
      421 gatcttcgaa ggccacaccg atttgcgccg gatcatgttg ccggaagatt ggtccggctt
      481 cccgctgcgt aaagattaca tcgaacagga agaatatgac ggcattagca cccaacgtgc
      541 gcccctggtc gaaaagttgt tgcgatagga tttaacgatg agcgaaaccg ccgctacgga
      601 tccaggcttc tacgccgaag agatgaccat cagtgtcggg cctcagcatc cgagcaccca
      661 cggggtgctg cgcttcgtgg tcaaaaccga cggtgaaatg atcaatgaag cgatccccga
      721 tgtcggctac ttgcaccgct ccatcgagaa gatcggcgag aaagtgacct atcacggcta
      781 tgtgccgtac accgaccggg ccgactatct ggccgcaatg tttgccaacc aagcgttctg
      841 catggcggcc gagaagctcg gcaacaccga agtgtcgcgc cgcggcgaat tttgccgggt
      901 catcgcttgc gaactcaatc gtctggccag ccatctgatt tctgtcggta cattcggcca
      961 ggatatcggt gccatgacac ccttcgtgca tgccctgcgc gagcgcgaaa ccatcaataa
     1021 tttgatggaa gagatttgcg gcgcccggct cacgtacaat tatatccgca tcggcggcgt
     1081 cggttacgat atcgtgccgt cgaccatgac caagatcgcc gagtttctcg atcacttcga
     1141 gcccatcgtc gaagagttca accgtttgct atccggcaat aagattttta tcgagcggtt
     1201 ggctcatgtt gcggtgatca gcaaagccga cgcgctgtcg tacaatctgg tcggacccaa
     1261 tctgcggggt tcgggcatca agtgggatat ccgccgcgat attccatatt ccgtctatcc
     1321 tgagttggat ttcgaaattc ccgtcggcac cgccgaggtc ggcactctgg gcgatagctt
     1381 cgatcgcttt tgggtgcgta ttcgcgagat gcaggagagc gtcaagatcc tgcgccaagc
     1441 gatgagaatg attcccaagg ggccggcgat ctcgaaagtg ccgaaaaaat tccgcccgcc
     1501 ggcgggcgaa tattacgccc gggtcgaagc gccgcgcggc gacatgggct tttacgtgat
     1561 cagcgatggc agcgagtatc cctaccgggt gcgcatccgt accggttcgt ttaccgcgat
     1621 ggccatcatc gataagctcg ccaaagggat catgatggcg gacttgatcg cgctcatcgg
     1681 cagtctcgat gtcgatgcgc cggaaatcga ccggtagaat ttatttgaga ttcatatgca
     1741 agcttttctc gaccaattga tccaagacgg cgcctttgcc ggtcttccta aagaagtcgt
     1801 ctacgccatc ggcatggtgc tggcggcagt ggtggtattg tcggtgtttg tcgcgccgct
     1861 ggccggcgtg accagctggc ttgagcggcg cgtctgggcg cgcatgcagt cgcgcgtcgg
     1921 ccccaaccgg gttggaccgc aaggcatcct ccagtggctc gccgatggca tcaagaacat
     1981 catgaaggaa gacattattc cggcggcggc ggatcaaaag ctctttccgc tagcgcccta
     2041 tattgttttc atgggcttcc tctgcgcgtt cgtagtcatt cctttcggcg aaaccctgat
     2101 cgtcgccgat ctcaacatcg gtatccttta cattctcgcg gtaacgtcgc tggtggtggt
     2161 cggcattctc atggccggct gggcatcgaa caacaaatgg tcgctgctcg gcggcatgcg
     2221 ctcggccgcg cagatcgtca gctatgaaat acccgcgggc ttgtcggttc tgacgatcgt
     2281 atttctcgct ggcacattga gcatgcaggg catcatcaaa gcgcagggct ggggtccttg
     2341 ggattggttc atatttcaca acccatttac attctgcgcg ttctttctgt tcttcaccgc
     2401 ggctttggcg gaaggcaacc gcacgccctt cgatattccc gaggcggagt ccgaattggt
     2461 cgccgggtac gtgaccgaat atagcggcat gcgctttctg tttttcttct ttgccgagtg
     2521 gggcaatctt tatgtcatcg gcgccgtggc gacgacgctc tttatgggcg gctggcaggt
     2581 gccggcgttt tttgacggct atcctgtgct caagggcatg gcgcagttcg cgacattctt
     2641 cttaaaggct tatttctggg tgtttgtggc gatgtggatt cgcgcgacct tgccgcgggt
     2701 gcgggtcgac caactgatgg gactctgctg gaagtacatg gtgccgttgt cgttcgtttg
     2761 tctgctcggc accatcggct ttatgttcat cgacgagatt agccggcgcg tctttggcgc
     2821 gatcacactg ggcttcgccg tcgccaccat aataaatttt ttccgccggg tcggctttca
     2881 atttcgcgcc gccaagccgg aagcttactg gaagccgcat atctagaatt tgaggattta
     2941 tgggtgttct taccagttat gtaaaagata ttcaggctgc gatcgcgtcg acctttgaag
     3001 gcatgtcgat caccttctcg catttgatgc gcaagccgat gacgatccag tatcccgata
     3061 agattcccgt gccgatccag gagaccttgc cgctgcgcta ccgcggcatc ctcgaagtcg
     3121 atttggatat ttgcaccggc tgtctggctt gcgaacggat ctgtccgatc acctgcatca
     3181 ccatcggcat cgacatgaac aaggagacca aacagcgcca gttcacccat ttcgacatcg
     3241 atgtctgcaa gtgcatgtac tgcggcctgt gctcggaggt ctgcccgacc ggagcgatcc
     3301 gccactcgca ggagttcgaa ggcgcgagct ataacccggc cggcctggtg cgccatttcg
     3361 cgcccgagcc gtcgaatctt tacaagccga aaaagggcgg tgaaaccgag cccaaattaa
     3421 ttaccaaagt cgatatcggt ttaaaatatc tggacgaatt tgctacgccg gatggaggag
     3481 gggccgccga ggaggaagca aaataggcgt ctggagcggg gcggtttgta tctctacagc
     3541 cgagcccctt gatctgttac caatcttccg ataagtcagc gaaaacatct gcaattttcc
     3601 gcgtttccaa aatttgagta aaggtatgac tgtttcaacg gcggtttttt atctaattgc
     3661 cctgctcacc atcgtgtcgg ccggcatggt ggcgttctcg cgcaatataa tttactcggc
     3721 gttctccctg ttgggcacgt ttatgggaat cgccggcttg tatattttcc ttggcgctga
     3781 cttcgtcgcg gcggtgcacg tgttgatcta cgtcggcggc atcctggtgc tgatcctgtt
     3841 cgccgtcatg ctcacccacc ggatcaccga tgtgacgatc accaaccgcg ccgccggcac
     3901 catccctgcg cttatcgtca tcggcgtgtt ggtttatttt ctgattcaga cggtgtggac
     3961 cacgccttgg gtcaaggcca aggaagtcgt tttcactgct acgacggcga agatcggcga
     4021 cgcctttctc tatgactact tgttgccttt cgagcttgct tcgttggtgc tgttggcggc
     4081 gatgatcggt gcggtggtgt tgtcgcggaa ggagatcaaa gactagccat gtcgcccaat
     4141 gagctgtctt cgtacctgac catcggcgcg atccttttcg cgctcggctt gttcaccgtt
     4201 ttgacccggc gcaatgccat cagcgtgttg atgggcgtcg agctgattct caacagcgcc
     4261 aatgtcaatt acgtggcgtt ttcccatttc agcagcggca atagcgccgg gcagatttac
     4321 gcgatctttg tcatcatgtt ggccgcggct gaggcggcgg tgggtttggc gatcgtcttg
     4381 gcgatcttca aattgttcca caccatcgac gtcgaagcgg cggaaacgat gagagattag
     4441 gacaacggtt cgaattcaca agctcaaggt cacaacgctc gatggaaaac cccatacaaa
     4501 cggatttcat tcgctggatc gtgttcctgc cgttgctcgg cgccatggtc aatggcctgc
     4561 tcggcgccaa gattcaaaag agcttgggca agggcgccat cggcgtgatc gcctgcgcgc
     4621 cggtgatcgt tgcctttgcg cttgcggttt acgccttttt catcctcaag gggctggcgc
     4681 cggagcagcg ctttctcatc gataatcttt atcgctggat cgatctcggc tcgctgcggg
     4741 tcgacatggc gtttctcgtc gacccgctgt cgtcggtgat gatcctggtg gtcaccggca
     4801 tcggcgggct catccatatt tacgccaccg ggtacatgca tgacgacaag gcgttctggc
     4861 gcttcttcgc ttacttgaat ctattcactg cagcgatgtt gaccctggtg ctgggcgata
     4921 gcctgctcct gctgttcgtc ggttgggaag gcgtcggtct gtgttcctac gcgctgattg
     4981 gcttttggta tcacgaccat gccaatgccc gggccggcaa caaggctttt atcgtcaacc
     5041 gggtcggcga tttcggtttt gtcctcggca tgtttttgct gttctggtcc ctcgatgccc
     5101 agggccatgg ctcgttgacc attcgcgaga tggccaaatg ggcgccgtcg atcaaggata
     5161 cgctcatttg gggttggccg gtcgttaccg tcgcgacgct gtttcttttc gtcggcgcca
     5221 ccggtaagtc ggcgcagatt ccactcttcg tttggctgcc tgatgcgatg gccggaccga
     5281 cgccggtcag cgcgctgatt catgccgcca ccatggtcac cgccggcgtc tacatgaccg
     5341 cgcgcatgaa cgttttcttc tccatggcgc cggcgacgtt gcacttgatc gcatggatcg
     5401 gggtcgcgac cgcgatcgtc gcggcgacca tcgcgctgac gcaaaacgat ataaaaaaag
     5461 ttctcgccta ctcgacggtc agccagcttg gttacatgtt catcggtgtc ggcgtcggcg
     5521 gatacgcggc cgcggtattt cacctcatga cccacgcttt tttcaaagcc tgtctctttc
     5581 tcggcgccgg cagcgtaatt cacgccatgc accacgagca ggacatgcgc aagatgggcg
     5641 gcctgaaaaa gtggatgccg attaccttcg cgaccttctt tatctcggtg ctcgccattg
     5701 ccggcttccc acccttcgcg ggctttttct ccaaggatga aattctctgg ctggcgttca
     5761 ccagcgaaca tggcaacaag gcggtctggt tcctagccct gtgcggcgcg ggcatgacgg
     5821 cattctatat gttccgccag ttgttcatga ctttttacgg cgagtgccgc gccgatcacc
     5881 atacccagga acacttgcac gaatcgccgc cggtgatgac catgccgctg gtgcttttgg
     5941 ccatcggcgc ggtattcgcc ggtttgatcg gtttgcccgg cgtcttgggc ggcagtcagt
     6001 tcgcccactg gctggagccg gtgattcacg cccatggcga aagccatgcc tcccacgcgc
     6061 tcgaatgggg attgatggcg gtttcggtgg ccgtcgcatc cttcggcgtt ttcatcgcct
     6121 ttctcatgta ccggcggcaa tccctctcgc cggatacttt cgtcaatctc gcgggcggcc
     6181 tgccctatcg attgttcgat cgtaaatggt acttcgatga gatctatcaa gcggttttcg
     6241 tcaacggcac tttgctgctg gcgcgcttct ggtcggcctt cgatgcctac atcgtcgacg
     6301 gcatcgtcaa cggcgtggcg accctgaccc gcttcgtcgc ctggttcaac ggactgttcg
     6361 atacctacat catcgacggt ctggtcaatg gcctttccaa cgttacttac tggctgggca
     6421 atcgattccg ccggctgcag accggtaaca tcaacggcta tctctacggc atcatcatcg
     6481 ccatggcggt ggcggtgttc gtcaaagtcc gatactggag ctgattttta ctggagcatg
     6541 gagaagatgc aaaatattct cacctacatg acgtttattc cgctggcagg agcggcggtg
     6601 gttctggcgt tgcccaacaa cgccaagctg gtccgctggt tttccgccat cgtcaccgta
     6661 ccgccgctgc tgatggcgat ttggctgttc atgaatttcg accgtaccac ggataatttt
     6721 cagttcgttg aagattatca ttggatccgc gcctacaaca ttagctacac catgggcgtc
     6781 gatggcctga gtatcaccat ggtgctgctc acggcgctgc tcagctttct ctgtatcttc
     6841 gcctcgtttg gcattgaaaa ggcggtcaaa ggttacttcg cgctgtttct catgctcgac
     6901 gccggcatga tgggcgtgtt ttgcgcgctg gacttctttc ttttctacgt tttctgggaa
     6961 gtcatgctct tgccgatgta ttttctcatc ggcatttggg gcgggccgcg gcgcgaatat
     7021 gccgccataa agttttttct ctacacctta ttcggcagcg tcttgatgct gttggcgatt
     7081 ctcgcgcttt attttaccgt cgaaccgcat accttcgata tgcgtatcat gatggcccag
     7141 gcgagcaagt acagcaccgc ggtgttgccg atgtggccgt ttaacggctg gggttggagc
     7201 ttccagcacg cgatttggct ggcgttgttt atcggcttcg cgatcaagat cccggcgttt
     7261 ccgtttcata cctggttgcc cgacgcccat gtcgaggcgc cgacggcgat ctcggtgatc
     7321 ctcgccggcg tgctcttgaa aatgggcacc tacggcatcc tgcgcgttaa ctttcccatg
     7381 ctcccggtgg cgaccgccga cttggcctac tggtttctcg gcgcgctcgg cgcttggaat
     7441 attatctacg gagccttctg cgccatggcg cagaaggatt taaaaaagct ggtggcctac
     7501 tccagcgtca gccatatggg ttacgtcatg ctcggcatgg cgagcttcac cacccagggt
     7561 ttgaacggcg cggtgttgca gatgttcaac catggcacca tcaccggcat gctcttcctt
     7621 ttggtcggcg tggtctatga ccgggcgcat catcgcgaga tcgacggttt cggcggcttg
     7681 gcggcgatca tgccgattta caccgggata accgccctgg cattcttcgc ttccatggga
     7741 ttgcccggcc tctccggttt catctcggaa atcctggtgc tgctcggcac ctggcagaag
     7801 tatccggtac tgacgatcct cggcgccagc ggcgtcattc tcaccgccgg ttatctgctc
     7861 tggacgatcc aacgggttta cctgggaccg cccaatgaaa agtatctcac gatggccgag
     7921 atcaacggcc gcgagatctt caccctcgtg ccactgggca tcatcgtcat catcgtcggc
     7981 gtctatcccg gcgtggtgct cgacatgctg cgcacgacct tggattcact caacgcaatc
     8041 gtcattccgc acctgcacgg ctagaccatg gatctgaaga acgtcgcgag cctcacttac
     8101 ttttgtccgg agatcgttct atctgtgacg atcttgctga tcattttgct cgatctgctc
     8161 gcgcagagcc ggcgttggcc ggcggcggtg gcgctcgtcg gctgcctggc cgcattggtt
     8221 gtttccttcg acctgtacag cgcccagcca ggcctgctgt tccatcgcat gatcgtgctc
     8281 gacaatttcg ccatcttctt caaagtattt tctctggcgg cggcgatcct gtgcatcctc
     8341 atgtccatgg gcagcaaaga gatcgaccga gtccatggcg ccgagtatta tgctttgctg
     8401 ctgacctgca ccctcggcat gtttttcatg gcatcgtcga gcaatctctt gatggcctac
     8461 ctgtccctgg aaatggtcag cctcggttcc tatgtcctga ccggcttctt gccccatagc
     8521 cgtcgttcca gcgaagccgc gctgaaatat ttaatctacg gcggcgtcgc ctccggcacg
     8581 atgatctacg gcatgagctg gatcttcggc atgaccggca gtctggacta tggggcgatt
     8641 caggttgccc tgagccaaga gggactgcac aaagtcgcgc tgtttatggc gtttattttc
     8701 atcctggccg gctttggcta caagatcgtt ttctttccgt tccatatgtg gtcgccggat
     8761 gtctatcaag gcgcgccgac gccgttcacg gcgttcctct cggtggcgtc caatgctgcc
     8821 ggcctggcga tcatgatccg tttctttttc cccggtgtct ccaagctttt accgtcgggg
     8881 gattgggcct tcacggcggg tgtcgaatgg ccgcaagtgc tgctggccct cagcatgctg
     8941 accatgacca tcggcaatct ctgcgcgctc aaccaacgca acgtcaaacg catgctcgcc
     9001 tactcgggca tcgcccacgc cggttacatg atgatgggct tggcgatact caataacgaa
     9061 gggctggcgg cgattctctt ctatgcggtg gtctacttga tcatgaacgt cggcgccttt
     9121 ctggtcgtcg gcatgatcgc caacgtcacc ggtgatgaag acatcgacga ttaccgcggc
     9181 ctcgcatggc gcggcgccac ggtaccggcg atttgtttgg cgatctttct tttctcgctg
     9241 acgggtctgc cgcccttcgc cggttttatc ggtaaatttt tcttgttttc ggcggtaata
     9301 aagcaaggcg gcgccttcgc aatcctggcc atcgtcgccg cgctcaacag cgtcatatcg
     9361 ctttattact atgccaagat cgtcaagatc atgttcctcg atttgccgct gccggaagat
     9421 aagcaaatct ccctggccag aaacgacttc gctttgctcc taccgttaac cgggttaacc
     9481 atcctgctcg gcgtctactt cggaccgctg gtgcagtaca ccaaccacgc gctgcagttc
     9541 tacaccaagt agttttccac ggcgcaaaat ttattgaatt taaaggcgct ggcggcggaa
     9601 ctcagatagc gctgttcaag ctcgcgacgg cgggatgata ccggcgatct tgtcggccgc
     9661 ggcggtgact tcgtccaggg atgcacggat tttttccagg gcggcttcgt cggacatcag
     9721 tttggaaacc gcgaacggtt cgccataggc gacgacgttt ttcgaaaatg gttttggtaa
     9781 gagataacga tcccaggagc gcagaaacag cggccatgag ctggagaaag ttatcggcag
     9841 aatccacgct ccggtgatgc gcgccaagcg cacgacgccg gcttgaacga catgaaccgg
     9901 tccgcgcggc ccatcgacgg tgagcgcgac gttgtggccc aaacgcactt ggcggaccat
     9961 gcggcgcaaa gcttcgctgc cgccgcggct ggtcgaccca cgagtgacca agtatccgtt
    10021 gttttcaagc cagcgggcga cgatatcgcc gtcgagcgac ttggaagcca tggtgacgat
    10081 gctttggtgc cggtgggcat cgatgggtaa atacatccgg ccatgccaaa gggcaaacag
    10141 ga
//
LOCUS       DASQKS010000181        16387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_96447, whole genome shotgun sequence.
ACCESSION   DASQKS010000181 DASQKS010000000
VERSION     DASQKS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96447"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..711)
                     /locus_tag="VE170_04615"
     CDS             complement(295..711)
                     /locus_tag="VE170_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HYJ14747.1"
                     /translation="MPVFSCSGTRPADLGVKDGRLLPCPASPNCVSSDAPDPAHSVAP
                     FQLVVPSTDGWRAARAAIDGLPRTKIITSTDDYLHAECSSAVFGFVDDLELHLRRGEN
                     LIAVRSAARLGHGDFGVNRKRVEQLRALLLRQGVVG"
     gene            complement(781..1158)
                     /locus_tag="VE170_04620"
     CDS             complement(781..1158)
                     /locus_tag="VE170_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYJ14748.1"
                     /translation="MATLRGIYFMSDHYHDENDLKLMREMRKLAPEEFNAWLGLNNIV
                     GRDNGAIPKKYRELIAIAVSATTQCPYCMEAHAKAAHAAGATREEVVEASFLAAALRA
                     GGAATHGAMALKFFDQQGEPKKT"
     gene            1230..2342
                     /locus_tag="VE170_04625"
     CDS             1230..2342
                     /locus_tag="VE170_04625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ14749.1"
                     /translation="MHVHSRPQLAAQCSVCGTMLSGALSAVYRTFGIKRSPRNPNICT
                     RCSTHVEEGKMVEITVLFADLSSFTELTQDLGAEKTHEVVDAFLRMATEILVKHGAFI
                     DKYVGDAVMALFNVPIRHDDHPLRAVSAATELSAALKILGADYNLDLTASIGIATGFA
                     RVGRLGSDDSKDYTAIGDVVNLAARLQGKASAGEVLISEESFAKHPTEFGEAKSEQIA
                     LKGFRQPVTAYRLLGSQATRLISDPPESLSHETTSMGAILFGILGAPCAVVTLIGPLA
                     VALGAGSVFGLAGLLTFLDQSMLRVPVLAFATLAAIGNLYILSHARKLRRDAKVPAQL
                     QTMTALEQRRTTFVLTTSVVTLGIVAFEVVAHIMLH"
     gene            complement(2425..2730)
                     /locus_tag="VE170_04630"
     CDS             complement(2425..2730)
                     /locus_tag="VE170_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYJ14750.1"
                     /translation="MLTQVSRYGEMMKVKVDKMKIYVTTRFRAEGSVLRETIKSGGVG
                     VELRSEIASSAPAERVAALIRNAEAGCYIMQTILNPTPVTREFSLNGESFDPESYKK"
     gene            complement(2731..2916)
                     /locus_tag="VE170_04635"
     CDS             complement(2731..2916)
                     /locus_tag="VE170_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14751.1"
                     /translation="MSDEMTVNRDIVIEADAESIERLRKEGHVRGFTVFCDEQQRTGG
                     DNSAPSPMGYFALSVAF"
     gene            complement(2945..3235)
                     /locus_tag="VE170_04640"
     CDS             complement(2945..3235)
                     /locus_tag="VE170_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002198731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HYJ14752.1"
                     /translation="MLTSAVGRLRAVGMIEAVSFLLLLGVAMPLKYFAGMPAAVKIAG
                     WIHGLLFITFGLCLLVAHGERRWSMRWSALVLVAALLPFGPFVIDRRLKSEQ"
     gene            complement(3237..3641)
                     /locus_tag="VE170_04645"
     CDS             complement(3237..3641)
                     /locus_tag="VE170_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ14753.1"
                     /translation="MAEQTLQGSCLCKSVRYEVTTPFMRFGHCYCSRCRKATGGVRST
                     NIAVPIAQFRWTQGENLIRRFDLPEAKSFARQVCDNCNCPIPHASRDGTRALVPAGTL
                     DDVPPGKPSVHGHWSSRVSFVENNEADLPCEN"
     gene            3856..4584
                     /locus_tag="VE170_04650"
     CDS             3856..4584
                     /locus_tag="VE170_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ14754.1"
                     /translation="MADPVLVTKENDITTILLNRPEVGNRQSDATWAQVTQLFDDAAK
                     SSRLIIFKGAGDDFCLGRDSMGVAPPTPGPEAYAVRERSETIFNLYGAFRNAKVPIVG
                     VVQGRAVGLGCALAALCDITIASDRARFSFPETAHKIMPTIAFSALVDRMPRKAATFM
                     IYSAQEIDAAKALTYALVSDVVKHGELDASVNRLVDHFKSMPIAAVLAVKEYARTAFS
                     LGTQAATDFARNLHATVNSYSGMR"
     gene            complement(4654..5061)
                     /locus_tag="VE170_04655"
     CDS             complement(4654..5061)
                     /locus_tag="VE170_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ14755.1"
                     /translation="MPRNIITAASLPASPDKIFDMYLDGEAHAAFTGLPVTLEPRAGG
                     LFRAFDGMISGTILYVLPKSLIVQTWRSATWPLTAMDSVLTLTFWPAEDGARIELVHV
                     NVADEDFAGVSQGWEKYYWTPWRAYLTAPTQST"
     gene            complement(5349..5945)
                     /locus_tag="VE170_04660"
     CDS             complement(5349..5945)
                     /locus_tag="VE170_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYJ14756.1"
                     /translation="MKIIFDLGGVVVRWEPAALLAQIFGDPATCNVLHAEFIGHADWL
                     ELDRGTMAPEDAVARAARRTGLSEQTVRHFLQKVPASLVPIPETVGLLHKLRDRGHSL
                     YCLSNMHFASIEYLERAHDFWDVFSGKVISCRIGLCKPETKIYAHLLDAYQLDAADTV
                     FVDDVEVNLAAARPFGIRTIRFESSAQCAEELRLLGCL"
     gene            6384..9023
                     /locus_tag="VE170_04665"
     CDS             6384..9023
                     /locus_tag="VE170_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HYJ14757.1"
                     /translation="MTQEELRLQQDLVRKANWKRWGPYLSERQWSTVREDYSPDGAAW
                     NSFPHDHARSRAYRWGEDGLGGISDRHQYICFSLALWNGQDAILKERLFGLTGNEGNH
                     GEDVKEYYFYLDSTPTHSYMKFLYKYPQAEFPYAWLVEENRRRGKGDLEFELVDTGIF
                     DDNRYFDVVIEYAKASADDILIRIEATNRGTAAAPLHLLPTVWFRNTWSWGLDARKPR
                     ANRDDTVDGTAIKLVHNYYGNRWFYCEGKPELLFTENETNAQRLYGLEDESPYVKDGI
                     NDYIVHGATQAVNPALTGTKAAAHYQMSVGPGQTIALRLRLSDSAPGNLNALDGKFDE
                     LLNQRRFEADEFYATVIPEDASADRKNVMRQALGGLLWSKQFYYYDVNRWIKGDPGCA
                     APPKERANGRNHEWRHLYNADVISMPDKWEYPWYAAWDLAFHCVALAIVDSAFAKEQL
                     LLMTREWYMHPNGQLPAYEWAFSDVNPPVHAWAAWRVYKVEMKRRGEGDRTFLERIFQ
                     KLMLNFTWWVNRKDAGGRNVFQGGFLGLDNIGVFDRSAPLPTGGYIEQSDGTSWMGMY
                     CLNMLAIALELAKENPAYEDVASKFFEHFVYICNAMNNLGGENIELWDAQDGFFYDVL
                     SLPNGQRSHLKVRSLVGLIPLFAVETLDPGVVDNLPGFKRRLQWFIENRPELSEHIET
                     ESTDDGPRRFLSLVSRHRLKRVLHYLLDENEFLSPYGIRAISRYHRDHPYTLKANGNE
                     YRVSYEPAESSTGMFGGNSNWRGPIWFPVNYLIIESLQKFHYYLGDNYKVECPTGSGN
                     LLNLWQIAGELSHRLTRIFTEDQNGHRPVFGGTEKFSNDPYWRDLIPFYEYFHGDNGA
                     GIGASHQTGWTALVAKLIQQYGE"
     gene            9308..10633
                     /locus_tag="VE170_04670"
     CDS             9308..10633
                     /locus_tag="VE170_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14758.1"
                     /translation="MTDTDRQRTLYPALCLGILTAAFIIAKTGRDALFFQGKGIFQLP
                     VATMMITAASLPLAMLFVKAMKTFGARPARVGLMLVAATVLGCAAPFLQPGESSLLFN
                     VFIFIPGVFGIMFASLWLLASDIFETTPKPQSARAFGKIGASSLAGGMVGGVIAKGIG
                     PRVEPKWLIFIGALMILGAIALVIQAHRKFPSNIAPKSDTGKKSNYLAPLAKPYAIGL
                     LFIAMSGALAGLLIDVQFYISATSAGMDSKGNANYFANFYIMLNFGSLLLQLFAAPKI
                     QDKIGIGGGLMILPFALLGGAGFASAAATAFSLSVLRITEGGLRSSIHRSIWEQAFTP
                     VDPGERSLVKIAVDGVGARIAEGIAAAGLFLWVQRVAPGGNLSGPLDTRWLAWLTVVT
                     VMVWLVVTQRLRAQVNQSAPAAGTAKFEVECTRFPDQCPCTTELGKGVA"
     gene            10968..11876
                     /locus_tag="VE170_04675"
     CDS             10968..11876
                     /locus_tag="VE170_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263046608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HYJ14759.1"
                     /translation="MRICSLLPGATEIVFALGLGDEIVGVTHECDYPADAQNKPVVVK
                     SAVDAKSMSGAEIDRKVVELLQAGKGLYRIDEVAFAEAAPDLVITQALCEVCALDYNE
                     VVEAAAALSHKPEIVSLNPHSLPEILDDILRVGTATGRSEAATALVDNLRQRIEQVGG
                     REPDRRPRVACLEWFEPLYSAGHWVPEMVALAGGHDVIGHAGEPSAKIDWRAVVDAQP
                     EVILLMPCGFDVRRTVKESTPLRALPGWNDLPAVRAQRVFALNGNAYFSRPGPRLVNG
                     LEILARIIHPDAVEWTLGPADAARVN"
     gene            12050..12682
                     /locus_tag="VE170_04680"
     CDS             12050..12682
                     /locus_tag="VE170_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ14760.1"
                     /translation="MAKTILVTGGAGGICSEISRGLAADGLNVVVADYAQDGAEKVAA
                     EIRDAKGSAIAVQVDVGDPKSVASMMEQAVAKYGQVDNIFCGAGVMDRVPVVEMPEEM
                     WDRLMRINLKGVFLCAQAAARHMMPRNEGRILSIASGRGVAGQPRSAHYAASKAGVIA
                     FTKSLAVELAPTTSRSTAFARAQPIHRCRALGLPTISSRNAKRFRRYWTA"
     gene            complement(12920..13306)
                     /locus_tag="VE170_04685"
     CDS             complement(12920..13306)
                     /locus_tag="VE170_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYJ14761.1"
                     /translation="MSTYKFSPQSARTGSAGFTLLELLVVVAVLGILAAIALQNLATH
                     RARAVDASMRSDLRNAAMAMESYYGEFQVYPSVDTGLLLFGFRKTHSVTLTISVPTPS
                     TYILTAARPAGSKPTFSFDSSTGLVN"
     gene            complement(13432..14523)
                     /locus_tag="VE170_04690"
     CDS             complement(13432..14523)
                     /locus_tag="VE170_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14762.1"
                     /translation="MKNLNLNEASAYSVKSPYEIYQEWEGIPVHKNFIVPDLLALELG
                     DWQRSGGRAAFVNQDGAGGMCDTMVEEIAPGGQLKPQRHMFEKAVFILQGQGATTIWN
                     EGGKKHTLEWQKGSLFSTPLNTWHQHFNAQGSEPVRLISLTDAPVVINRYRNLDYIFN
                     NNFTFTDRYEGGADAWSGAGGRYLGPEIKRGRVWESNFIADLWAFEPKDYKERGGDNR
                     TTLFEFVDNTMSAHVSEFPIGKYKKAHRHGAGAHIVMLTGSGYSFLWPEGEYGAKRRV
                     EWGRMSMFTPPMQWWHQHFNAGGEPARYLALKPWGFKYKVEELKDTGEDVKNGGAQIE
                     YQDQNPEIHDLFLAECKKSGAEPRMPMFG"
     gene            complement(14559..15263)
                     /locus_tag="VE170_04695"
     CDS             complement(14559..15263)
                     /locus_tag="VE170_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ14763.1"
                     /translation="MSLVKDLCVQLDQMVNDQFDSVEFRALLDLPLTMERGRFYVVQN
                     ALYTSNRRDCWGYVQGGAPLDVKRVIWQHESDELINDPRAGMDHYNLTVKQGEVIGLK
                     REDFDNAQVPAMAQACFHAWHHIAMRSHWLTAYTASHMLERRNNGKIVKGGGMSFRVG
                     KKFENELGINLKRMISMDVHVAADMEHSENISEMFEKYAKTPADCERVLEGARESMAI
                     DRAYRGALGFYMERIN"
     gene            complement(15367..16083)
                     /locus_tag="VE170_04700"
     CDS             complement(15367..16083)
                     /locus_tag="VE170_04700"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ14764.1"
                     /translation="MSVHSTGLEQMWQELVRLCNEQFESQPFKRLLQSKFTKPRVQQY
                     SIQMAYYVQNRRDCWGYVQGSAPLPVKQIVWRHEEDELIGQKAQGKVDHITLAVKEGE
                     VFGLSAEAFETTARMEGGEVCFSAWIHLAQKSWLEAIAASAILEMRNSAELIRGGSLS
                     HRIAAIFERDLGIPMKKQINNAEHVVMDVEHAHLLMQVAKQYATTETAQEAILSGARE
                     SLLIDRVYRGHLADLMDSLD"
BASE COUNT         3531 a         5028 c         4487 g         3341 t
ORIGIN      
        1 ccggccggcc ggccatggta gctccgttcg agcccgatca gcccgccgat gaccaaggag
       61 gcgaacaagc gcaaagcgat gtcgaaattc acgccgtctg aaagtcgaat ttcagccatg
      121 gcatctccaa cttttgccag ctagagcgat cttgcaggtc cggcgtagct atcacgattt
      181 tggccgcgct aaaattcctg ccatcgagaa tcagccgggc tagcgacggc gaacctacac
      241 tctcgcggca ccttccggat gggtcttttg ggccttcgcc cgcgccaaca aatctcaacc
      301 taccacgcct tgcctgagca gcagcgcgcg caactgctcc acccgcttgc ggttaacgcc
      361 gaagtcgccg tgccctaaac gcgctgcgga acgcaccgcg atcaaatttt ccccgcgccg
      421 caagtgcagc tcgagatcgt cgacaaagcc gaacaccgcg ctgctgcatt cggcatggag
      481 atagtcgtcc gtcgaagtga tgatcttcgt acgcggcagt ccgtcgatgg cagctcgggc
      541 cgcgcgccag ccatccgtcg atggcacaac cagctgaaac ggagcgaccg aatgggcagg
      601 gtcgggcgca tcgctcgaca cgcagttagg cgaggcgggg cagggaagca ggcggccgtc
      661 tttgactccc aaatcggcag gtctggttcc cgagcatgaa aataccggca tagtaataac
      721 gcccgcggcg agaagccaga aaactttctg tatcaagtta gccaacacac gcacggcggc
      781 ctatgttttc ttcggctccc cctgctggtc gaaaaactta agcgccatgg cgccatgggt
      841 cgcggcaccg ccggcgcgca aggcggcggc cagaaacgat gcttcgacga cttcttcgcg
      901 cgtcgcgccg gccgcgtggg cggccttggc gtgggcttcc atgcagtagg ggcactgggt
      961 cgtcgccgac acggcgatgg cgatcaattc acgatatttt tttgggatcg cgccgttgtc
     1021 gcggccgacg atgttgttca gtccgagcca agcattgaat tcttccggcg ccagcttgcg
     1081 catctcgcgc atcaacttca aatcgttctc gtcatgatag tgatccgaca tgaaataaat
     1141 ccccctcagt gtcgccatat tattcccgca ttcggcacac gaagcaatca cgttgacatc
     1201 attgctaggg ctgcgctact tcttccctaa tgcatgttca ttcgcgtccg caactggcgg
     1261 cacaatgcag tgtctgcggc accatgctgt ccggcgcgct gagcgccgtc taccgcacct
     1321 tcggcatcaa acggagcccg cgcaatccaa acatctgcac ccgttgcagc acccatgtcg
     1381 aagaagggaa aatggtggaa atcaccgtgc tgttcgccga tctgtcgtca ttcaccgagc
     1441 tgacccaaga tctcggcgcg gagaaaaccc acgaagtcgt cgacgccttt ctgcgcatgg
     1501 ccaccgaaat cttagtgaag catggagcat tcatcgacaa atacgtcggc gacgcggtca
     1561 tggcgctgtt caatgtaccc atccgccacg acgaccaccc cctgcgcgcg gtatcagcgg
     1621 caaccgaact gagcgcagcg ctcaagattc ttggcgcgga ctataacctg gacctcacgg
     1681 cctctatcgg tatcgccact ggatttgccc gcgtcgggcg cctaggctcc gacgactcga
     1741 aggactacac ggcgatcggc gacgtggtta atctggcggc gcgcctgcaa ggaaaagcca
     1801 gcgccggcga agttctcatc agcgaagaaa gttttgccaa acacccaacg gagtttggcg
     1861 aagcgaaatc ggagcaaatc gcgcttaagg gtttccggca gccggttacc gcgtatcgtc
     1921 tgctcggcag tcaagctacg cggttgataa gcgatccgcc cgagtctttg tctcacgaaa
     1981 cgactagcat gggcgcgatc ctattcggca tcctcggcgc gccgtgcgcc gtggtcacgc
     2041 tgatcggccc actggcagtt gcgttaggag ccggcagcgt gttcggatta gccggcctgc
     2101 tgactttcct cgaccaaagc atgttgcgcg tgcccgtgct cgcgttcgcg acactcgccg
     2161 ctatcggcaa tctctacata ttgtcccatg ccagaaagct gaggagggac gccaaagttc
     2221 cggcccagct gcaaaccatg acggccttgg aacagcggcg cacgactttc gtgctcacca
     2281 cctcggtcgt caccctcggg atcgtcgcct tcgaagtcgt cgcccacatc atgctgcatt
     2341 gaaaccttat cctgcgcggg acagcaatat cgaagaccca cagctgcggc cttttgccgt
     2401 ggttctaagg ttgtcaggga ataattattt tttgtagctt tcaggatcga acgactcgcc
     2461 gttgagagaa aactcgcggg tcaccggagt cggattgagg atcgtctgca tgatgtaaca
     2521 acccgcttcg gcgttgcgga tcagcgccgc gacccgctcc gccggcgcac tcgacgcaat
     2581 ctcgctgcgc aattcaacgc cgacgccgcc cgacttgatg gtttcgcgca gcaccgagcc
     2641 ctcggcgcgg aatcgcgtgg tgacgtaaat cttcatctta tcgactttca ccttcatcat
     2701 ttccccgtac cgggaaactt gggtaagcag tcaaaatgcc accgacagcg cgaagtagcc
     2761 catcggcgac ggcgccgaat tgtcgccacc ggtgcgttgc tgctcatcac aaaaaaccgt
     2821 gaagccgcgc acatgccctt ccttgcgcaa gcgctcgatg gactccgcgt cggcttcgat
     2881 aacaatatcg cgattgaccg tcatttcatc agacatgtta gtctctcctc aatgtcgaaa
     2941 acgcttactg ttcgctcttg agccgccggt cgatgacaaa tggcccgaac ggcaaaagcg
     3001 ccgcgaccaa aaccaaggcg ctccagcgca tcgaccagcg gcgttctcca tgggcgacca
     3061 gcaagcacag gccaaaggtt atgaatagca acccgtgaat ccaaccggca attttcaccg
     3121 ccgcaggcat gccagcgaaa tacttgagcg gcatcgccac gcccaacagc aggagaaacg
     3181 aaactgcttc gatcatccca accgcgcgca gccgccccac cgcgctcgtc aacatgctaa
     3241 ttctcgcacg gtaaatccgc ctcgttattc tcgacgaaac ttacccggct gctccaatgt
     3301 ccgtgcacgg aaggctttcc cggagggacg tcatcgaggg taccggcggg aaccaaagca
     3361 cgggtgccgt cgcgcgacgc atgcgggatc ggacaattgc aattgtcgca aacctgccgg
     3421 gcgaaactct tcgcttcggg cagatcgaac cgcctaatca agttttcacc ctgagtccag
     3481 cggaactgcg cgatcggcac cgcgatattg gtcgagcgca cgccgcccgt tgctttgcgg
     3541 cagcgcgagc aatagcagtg gccaaagcgc atgaacggcg tagtcacttc gtagcgcacc
     3601 gatttgcaca aacagcttcc ctgcaaagtt tgttcagcca taacaccctc ctaatatcgg
     3661 catatggaat gacccggagt ctattttgaa atgaatttct gcgcaacaac gagtggtgtc
     3721 aaatcgacag atcctttgag ttaagacgaa atcaacgttt tgaacgactt gaacccgcga
     3781 ccacgcggct aaagccgttg acagctacgc caaacctctc ctaaagtgac ccgattaaat
     3841 cttggagaaa aaatcatggc agatcccgta ctcgtaacca aagaaaatga cattaccacc
     3901 atcctgttga atcgaccgga ggtcggcaac cgccagagcg acgctacctg ggcgcaggtg
     3961 acgcaactgt ttgacgacgc cgccaagtcg tcacgactga ttattttcaa aggcgccggt
     4021 gatgatttct gcctgggtcg ggactccatg ggtgtcgcac cgccgactcc cggtccggaa
     4081 gcctatgccg tgcgcgaacg tagcgagacg atcttcaatc tctacggcgc ctttcgcaac
     4141 gccaaagtgc cgatcgtcgg cgtcgtccaa gggcgcgccg tcggcttggg gtgcgctctc
     4201 gcggccctgt gcgatatcac cattgccagc gatagggcgc gtttctcgtt tccagagacc
     4261 gcgcacaaaa tcatgccgac catcgccttt tccgcactgg tcgaccgtat gccgcgcaaa
     4321 gcggccacct tcatgattta ctcggcgcaa gaaatcgatg ccgccaaagc gctcacctat
     4381 gcgctggtga gcgatgtggt aaagcatggc gaactcgacg ccagcgtcaa tcgcttggtc
     4441 gaccacttca agtctatgcc catcgctgcg gtcctggcgg tcaaagaata cgctcgcacg
     4501 gcgtttagcc tcggcacgca agccgccacc gacttcgccc gcaacttgca cgcgacggtg
     4561 aatagctatt cgggaatgcg ctaatcaaca gcggaaggcg gcgccgaact tgtggccata
     4621 cgggatcgtg attgccatcc tggcgatagg gaattatgtc gactgagtgg gcgcggtgag
     4681 ataggcgcgc cacggggtcc agtaatactt ttcccaaccc tggctgaccc cggcgaagtc
     4741 ttcatcggca acgttgacgt ggacaagctc gatccgcgcg ccgtcctccg ccggccaaaa
     4801 tgttagcgtc agcaccgagt ccatcgccgt cagcggccag gtcgccgacc gccaagtctg
     4861 cacgatcaac gacttgggca aaacgtacag aatcgtgcct gaaatcatac cgtcgaacgc
     4921 gcgaaacagg ccgcccgcgc gcggctcaag tgtcacaggc aacccggtaa acgccgcatg
     4981 cgcctcgcca tcgagataca tgtcgaaaat tttgtcagga gacgcgggca gcgaggcggc
     5041 tgtgatgatg ttacgcggca tagtagatag ctccttttta cgaggctaat cacccggcaa
     5101 tcacggcgtc accgtcgccg gtccaggcga ttctccgagc agcggacaaa tatcgaggac
     5161 aaacacacta atcgatgtga gtagaaaaac tgttgggatc cggctagtca acatcttgat
     5221 cgctcgggta ttgcaatcaa taaataattt cacccacctc tatcacactt cgccaaagtc
     5281 cggtaaggcg atgcgattat gcacgatagc gcttccggct cgaatcgccg aaacaaatca
     5341 cgagtatatt aaagacaacc caataatctc agttcttcag cacattgcgc tgaactttcg
     5401 aaccgaatcg tccgtatgcc gaacggccgc gcggcggcta aattgacttc gacatcatcg
     5461 acaaagaccg tgtctgcggc atcgagttgg taagcgtcca gtaagtgcgc gtaaattttc
     5521 gtttccggtt tgcataggcc gatccggcag gaaatgacct tgccgctgaa gacatcccag
     5581 aaatcatggg cgcgttcgag atattcgatg gacgcgaaat gcatgttcga caggcagtag
     5641 agcgagtggc cccgatccct taacttgtgc agcaacccaa ctgtctcggg aatcggcacc
     5701 agagacgccg gcaccttctg aagaaagtgg cgcaccgtct gttcggaaag tccggtgcgc
     5761 cgcgcggccc gcgccaccgc atcttccggc gccatcgtgc cgcggtcgag ctcgagccaa
     5821 tcggcatggc cgatgaactc ggcatgtaaa acattacacg ttgccgggtc gccaaagatc
     5881 tgcgccagca gcgccgccgg ctcccagcgc acgaccaccc cgccgagatc gaaaattatt
     5941 ttcatggtgc tcgctctaat ctcgtactcc gccaaaatga gacgaattgc taaattctac
     6001 ggcgcgcgct catgccgcac aagaacagtt gagagtttat ggcacgtaac cagtccaccc
     6061 attgaccgcg ttaacaatgc acatcgcgag tccaagccgc cacttgaaga ttgccgtaac
     6121 cgagctctag agatcatacg caagattcga cctgcatgcg cctgccgccg aaccgcacgc
     6181 accgtcggac cgctgcaact ttactgcggc cgtcgaaaaa ttgacagcag cacttcgcgt
     6241 tcgacaatgc tccggtcatg ctcgaatcgc cggtgcttga atgattttaa tattttgacc
     6301 gctggaacgc ctaatccccg ccccggtgat gaggatgtgc taaaatagct gttaagaacg
     6361 tttctcgatc gaggaaaaga ccggtgactc aggaagaact ccgactgcaa caggacctcg
     6421 tccgtaaagc caactggaaa cgctgggggc catacctttc cgagcgccag tggagcacgg
     6481 tgcgcgaaga ctatagccct gacggcgcgg catggaattc gttcccgcac gatcacgcac
     6541 gcagtcgcgc ctaccggtgg ggcgaagacg gtctcggcgg tatctccgac cgccatcaat
     6601 atatttgttt ctcactggcg ttgtggaacg gtcaagatgc gatcttgaaa gaacgcctct
     6661 tcggcctcac cggtaacgag ggcaaccacg gcgaagacgt taaagagtat tacttttatc
     6721 tcgactcgac gccgacccac tcctacatga aattcctcta caagtacccg caagcggaat
     6781 ttccttacgc gtggctggtc gaagaaaacc gccggcgcgg caaaggcgat ctggagttcg
     6841 agctcgttga caccggcatc ttcgacgaca atcgctattt cgacgtggtc atcgaatacg
     6901 ccaaggccag cgccgacgat atattgatcc gcatcgaagc caccaatcgc ggcacggcgg
     6961 cggcaccgct gcacctcctg cccaccgtct ggtttcgcaa tacctggtcc tggggcttgg
     7021 acgcgcgcaa accaagagcc aaccgggatg acaccgtcga cggcacggcg atcaaactcg
     7081 tgcacaacta ctacggcaac cgttggttct attgcgaagg taaaccggag ctgctcttca
     7141 ccgagaatga aaccaacgcg caacgcctct acggattgga agacgaatcg ccctacgtga
     7201 aagacggcat caatgactat atcgttcacg gcgccaccca ggcggtcaat cctgctttga
     7261 ctggcaccaa agcggcggct cactatcaga tgagcgtcgg gcccggccag acgatcgctt
     7321 tgcgcctgcg cctgagcgat agtgcgccgg gtaacctgaa tgcactcgac ggcaagttcg
     7381 acgaactgct caaccaacgg cgttttgaag cggatgagtt ttacgccacg gtgattcccg
     7441 aagacgcgag cgccgaccgc aaaaatgtca tgcgccaggc gttgggcggt ctgctctgga
     7501 gcaaacagtt ttactattac gacgtcaatc ggtggatcaa aggggaccct ggctgcgccg
     7561 ccccgcccaa ggagcgcgcc aatggccgca atcacgagtg gcgccattta tacaacgccg
     7621 acgtcatctc gatgccggac aagtgggaat atccctggta cgcggcatgg gatctggcgt
     7681 ttcattgcgt cgccctggcg attgtcgact cggccttcgc caaagagcaa ttactgctca
     7741 tgacccgcga gtggtacatg caccccaacg gccagctccc ggcctacgaa tgggccttca
     7801 gcgatgtcaa tccgccggtc cacgcctggg ccgcctggcg cgtctacaaa gtcgaaatga
     7861 aacggcgcgg cgaaggtgac cgcacattcc tcgagcggat ttttcaaaag ctcatgctca
     7921 acttcacctg gtgggtcaac cgcaaggacg ccggcggcag aaacgtgttc caaggcggct
     7981 ttttgggcct cgacaacatc ggcgttttcg accgcagcgc gccgctgccc accggtggct
     8041 atatcgagca atccgacggc accagctgga tgggcatgta ttgtctcaac atgttggcga
     8101 tcgcgctgga attggccaaa gaaaaccccg cctacgaaga tgtcgccagc aaattttttg
     8161 aacattttgt ctacatctgc aacgccatga acaaccttgg cggcgagaat atcgagctct
     8221 gggacgctca ggacggattt ttctacgatg tcttgtcgct gccgaacggc caacggtcgc
     8281 acttgaaagt gcgctcgctg gtgggcctga ttccactctt cgccgtggag actttggacc
     8341 ctggcgtggt cgacaattta ccgggcttca aacggcgctt gcaatggttt atcgagaacc
     8401 gtccggagtt gagcgagcat atcgaaaccg aatcgaccga tgacgggccg cgtcgttttc
     8461 tctcactggt cagccggcac cgactgaagc gcgtgctcca ctacctgttg gatgaaaacg
     8521 aatttttgtc gccctacggt atccgcgcca tttcgcgtta ccaccgcgac catccctaca
     8581 cgctcaaggc caatggcaat gagtaccgcg tcagctacga acccgcggaa tcgagcaccg
     8641 gaatgttcgg cggcaattcc aattggcgcg gcccgatctg gtttccggtc aattacttga
     8701 tcatcgagtc gctgcaaaaa tttcattact atctcggcga caattacaaa gtcgagtgcc
     8761 ccaccggttc cggcaacctg ttgaatctct ggcagatcgc cggcgagctg tcgcaccgcc
     8821 tgacgcgcat cttcactgaa gatcaaaacg gccatcggcc ggtgttcggc ggcacggaaa
     8881 aatttagcaa cgacccctat tggcgtgacc tgatcccgtt ctacgaatac ttccacggcg
     8941 acaacggcgc cggcatcggc gccagccacc aaaccggctg gaccgcgctg gtggcaaaac
     9001 tgattcagca gtatggcgaa tgattcgcgt gctctagagt tttcggttta ggtgccgcga
     9061 taagaccact ctcacagacc aactctcgct cgcacattaa agctgcatga gctgcaacgc
     9121 cgagtgcgtc tatgacacct aagcattgaa cagtttcgtt gccatcgtga tcacagcaca
     9181 tttcctttca ccgcaggaac tttagcgccg atgagttcgt tgtcgtcacg ggaggcgtgc
     9241 agaattaccg aaatttgccc caccggctgt gatttctgac atctgaccaa gacatccatc
     9301 aagcaccatg accgataccg accgacaacg cacgctctac ccggcgcttt gcctcggcat
     9361 attgaccgca gcatttatca tcgccaagac cggccgcgac gccttgttct tccagggcaa
     9421 gggtatattt cagctaccgg tggcgaccat gatgatcacc gccgcctcgc tgccgctggc
     9481 catgttgttc gtcaaagcga tgaagacctt tggcgcgcgg ccggcgcggg tcggcttgat
     9541 gctggtcgcc gctacggtcc ttggttgcgc agcgccgttt ctccagcccg gcgaatcgtc
     9601 gctgctgttc aatgttttta tctttatccc gggcgttttc ggcatcatgt tcgcgagcct
     9661 ttggttgctc gccagcgata ttttcgaaac cacgcccaag ccgcagtcgg cgcgggcgtt
     9721 cggcaagatc ggcgccagtt cgctggcggg cggcatggta ggcggcgtca tcgccaaagg
     9781 gatcggtccg cgggttgaac cgaaatggct gatcttcatc ggcgcactga tgatcctcgg
     9841 cgccatcgcg ttggtcatcc aagcgcacag aaagtttccc agtaatatag cgcccaagag
     9901 cgacaccgga aagaagagta attatctcgc gccgctggcg aagccctatg ctatcgggct
     9961 gcttttcatc gcgatgtccg gcgcactcgc cggcctactc atcgacgtgc aattttacat
    10021 atcggccaca agcgctggca tggactcgaa aggtaatgcc aactactttg ccaattttta
    10081 catcatgctc aatttcggct cgctactgtt gcagcttttt gccgcgccga aaatccagga
    10141 caagatcggc atcggcggcg gcttgatgat cctgcccttc gcgctgctcg gcggcgccgg
    10201 attcgccagc gcggcggcca ccgctttcag cttgtcggtg ctgcgcatca ctgaaggcgg
    10261 cctgcgctcg tcgatacacc gatcgatctg ggaacaagcg ttcacgccgg tcgaccccgg
    10321 cgaacgctcg ctggtcaaga tcgccgtcga cggcgtcggc gcgcgcatcg ccgaaggcat
    10381 tgctgctgcc ggcctttttc tgtgggttca aagagtagcg cccggcggca acctgtccgg
    10441 cccgctcgac acccgttggc tggcttggct gacagtggtg acggtgatgg tctggctggt
    10501 cgtcacccag cggttgcgcg ctcaagtaaa ccaaagcgcg ccagcggccg gcacggccaa
    10561 attcgaagta gaatgcacgc gctttcccga tcagtgcccg tgtacgacgg aattgggcaa
    10621 gggcgtcgcc taaacttttg ccatcagcct caccgcgcgg gcgcgacagc agagcgcgta
    10681 acggagcccc accaaagcac cgctccttca ccgtaaatga tcacttacga agtcatgccg
    10741 ggccaggtat aggcgcaagc gacgatacca tccgttgcca aaaatcgcct gcaccaccgt
    10801 cgttaacatg cctgcatcca tgtcgcgctt ttccccgcgc gctcatcggt aaggactcgc
    10861 gctccatcgg ctgcctgtgc tgttcggcgg tcggcaatcg tctcgaacga ttcatttttt
    10921 gatcgccgtc ggtagcataa agccggcgca tacgctatgc atcggccatg cgaatctgct
    10981 ccctgctgcc aggcgccacg gaaatcgttt tcgcactcgg gctcggcgac gaaatagtgg
    11041 gcgtcaccca tgaatgcgac taccccgccg atgcgcaaaa caaaccggtg gttgtgaaaa
    11101 gcgccgtcga cgccaagagt atgagcggcg ccgagatcga ccgcaaagtc gtcgagttgt
    11161 tgcaagccgg gaaaggtctg taccgcatcg acgaagttgc ctttgccgag gccgcgcccg
    11221 atctggtcat cacccaggct ttatgcgagg tttgcgcact ggattataac gaggtggtcg
    11281 aggcggcagc ggcgctcagc cacaagcccg agatcgtttc tttgaatcct catagcttac
    11341 cggaaatcct cgacgatatt ctccgcgtcg gcacggccac cggacggagc gaggccgcca
    11401 cggcgctggt cgacaatctg cgccaacgca tcgaacaggt cggcggtcgc gaaccggacc
    11461 gccgcccgcg ggtggcctgt ctggagtggt tcgagccgct ctacagcgcc ggtcactggg
    11521 tgccggagat ggtcgccctc gccggcggtc acgacgtcat cggtcatgcc ggtgagcctt
    11581 cggcaaaaat cgactggcgc gcggtcgtcg acgcgcaacc ggaagtgatc ttgctcatgc
    11641 cctgcggttt cgacgtccgg cgcacggtga aagaatcaac gccgctgcgc gccctgccgg
    11701 gctggaacga cctgccggcc gtgcgcgcac aaagggtctt cgccctcaac ggcaacgcct
    11761 acttcagccg gcccggcccg cggttagtca acgggctgga aatcctcgcg cgaatcattc
    11821 atccggacgc cgtagaatgg accctcgggc cggcggacgc ggcccgcgtc aattgattgg
    11881 cgcggcctga tttgtctgac tgcgccatcg ccaccccatg gtttagtgcg ctgcgatcag
    11941 atcaatattg gctgtgcctg gcatggcggg ggttcgaaaa gttgacccaa acaaaccgcg
    12001 atgatagctt cgccagactt atccattaga caaaagggag agcttcgtca tggcaaaaac
    12061 catcctggtc accggcggcg ccggcggtat ctgttcggaa atttcccgcg gtctggcagc
    12121 cgatgggcta aacgtcgtcg ttgcggacta tgcccaagac ggggcggaaa aagtcgccgc
    12181 cgagattcgc gatgccaaag gcagcgccat cgccgtgcag gtcgatgtcg gcgatcccaa
    12241 gagcgtagcg tccatgatgg agcaagcggt ggcgaagtac ggccaggtcg ataacatctt
    12301 ttgcggcgct ggcgtgatgg accgcgtgcc ggtcgtcgag atgccggaag agatgtggga
    12361 ccggttgatg cggattaact tgaagggcgt cttcctgtgc gctcaggctg cggccaggca
    12421 catgatgccg cgcaacgaag gaaggattct cagtatcgct tcgggccgcg gcgtcgccgg
    12481 tcaaccgcgc tcggcgcact acgccgcttc caaggccggc gtgatcgcct tcaccaaatc
    12541 ccttgccgtc gagttggcgc caacaacatc acggtcaact gcgtttgccc gggcgcaacc
    12601 gatacaccga tgtcgcgcat tgggtttacc gacgatcagt tcaagaaacg cgaagaggtt
    12661 ccgccgttac tggacggcct gacccataaa ttcgagatcg tcggactggt gcgctacctg
    12721 ctctcggacg cggcaaaata tattaccggg caaacttatt ttctgcgcac gccgaagtaa
    12781 cggcactagc ggcgggccca gcccacgcct aaaccatggc aatgtttcag gcgcacgcct
    12841 cgaccatggg gcggcgttgg caggcggatg cgggcaactg agcgggtgtg cgaagaaggc
    12901 gtcgaaccgg gccaagcgat cagtttacca gcccggtact gctgtcgaag ctgaaggttg
    12961 gcttgcttcc ggcaggacgc gccgccgtca gtatataggt cgacggggtc ggcaccgaga
    13021 tcgtcaacgt gaccgaatgg gtcttacgaa aaccaaacaa taagagtccg gtatcgacgc
    13081 taggatatac ctgaaactca ccatagtagc tctccatcgc catggctgcg ttgcggagat
    13141 cggagcgcat cgaggcatcg acggcacggg cccgatgcgt ggctaaattc tgcagcgcga
    13201 tggccgctaa aattcccagc acggcgacga caaccagcag ttccaataat gtgaaaccgg
    13261 cgctgccggt gcgggccgat tggggggaga atttataggt cgacatcgtt ggaggctcaa
    13321 ttaaattccc catattttac tgtacgggct aggacttgtc tagaaaaaat tcccccctat
    13381 ggtgttgccg ggtcggcgct atcgcttcca accccttgcg ccgaaaagtc actaaccgaa
    13441 catcggcatg cgcggctcgg caccgctttt tttacactcg gcgaggaaca ggtcgtgaat
    13501 ttcgggattc tgatcttggt attcgatctg agccccgccg tttttaacgt cctcgccggt
    13561 atccttcaat tcttcgactt tgtatttgaa gccccagggt ttgagcgcca gatagcgcgc
    13621 cggttcgccg ccagcattga aatgctggtg ccaccactgc attggcggcg tgaacatgct
    13681 cattcggccc cactcgacgc gccgtttggc accatattct ccttcgggcc agagaaatga
    13741 ataacccgaa cctgtcagca tcacgatatg cgcgccagcg ccatggcgat gggccttttt
    13801 gtatttaccg atggggaact ccgacacatg ggcgctcatg gtgttatcga cgaactcgaa
    13861 cagcgtcgtg cggttgtcgc cgccacgctc tttgtaatcc tttggttcga aggcccacaa
    13921 atcggcgatg aagttcgact cccacacccg gccgcgcttg atttccggcc cgaggtagcg
    13981 tccgccggcg ccgctccaag catcggcgcc accttcgtaa cgatcggtga aagtaaagtt
    14041 gttattgaaa atatagtcga gattacgata acgattgatc accaccggcg cgtcggtcag
    14101 cgaaatcagc cgcaccggct cgctgccttg agcgttgaaa tgctggtgcc aggtattgag
    14161 cggcgtcgag aacaaactgc ccttttgcca ttccagagta tgtttcttgc cgccttcatt
    14221 ccagatcgtc gtcgcaccct gcccttgcag gataaaaacc gctttctcaa acatgtgacg
    14281 ctggggcttc aactgcccgc cgggagcgat ttcttcaacc atcgtgtcac acattccccc
    14341 cgcgccatct tgattgacga acgctgcccg gccaccgctg cgctgccaat cacccaactc
    14401 cagcgctaac aagtccggca cgatgaaatt cttgtgaacc ggaatcccct cccactcctg
    14461 atagatctcg tagggactct tcaccgagta tgccgacgct tcgttcaaat tcagattctt
    14521 catcgttgcc ttctccttat cagcctgccg aatgaaaatt agttgatccg ctccatgtag
    14581 aaacccagag caccgcggta agcccggtcg atggccatgg actcgcgcgc gccttccagc
    14641 acccgttcgc aatccgccgg cgtcttcgca tatttctcga acatttccga aatattttcg
    14701 gagtgctcca tatcggcggc gacatggaca tccattgaga tcatgcgctt gagattgatg
    14761 cccaactcat tctcgaactt cttgccgacg cgaaacgaca tgccgccgcc tttgacgatc
    14821 ttaccgttgt tgcggcgctc gagcatgtgc gaagcggtat acgccgtcag ccagtggctg
    14881 cgcatggcga tatgatgcca ggcatgaaag caagcctgcg ccatggccgg cacctgggca
    14941 ttgtcaaaat cctcgcgctt gaggccgatg acctcgcctt gttttacggt caggttataa
    15001 tggtccatgc cggcgcgagg atcgttgatc aattcatcgc tctcatgctg ccagatcacc
    15061 cgcttcacgt ccaaaggggc gccgccttgg acgtagcccc agcaatcccg ccgattgctg
    15121 gtgtagagcg cgttttgcac gacgtaaaat cgtccgcgct ccatggtcag cggcaggtcg
    15181 agtaatgccc tgaactcgac gctgtcgaac tggtcgttga ccatctggtc gagctgaaca
    15241 caaagatcct tcactagact catcgttcct ccagtcaaat atcagcgggc gggcttgcta
    15301 tccgtcccgc acgagaaggc gagcgctttt ttgcgccgtt gccgatccaa cactagctcg
    15361 gaccgttcag tccaaactat ccattaagtc ggccaaatga ccgcgataga cgcggtcgat
    15421 cagcagactc tcgcgggcgc cgcttaggat cgcctcttgg gccgtctcag tggtcgcgta
    15481 ctgcttggcg acctgcatca acaggtgagc atgctcgacg tccatcacca cgtgctcggc
    15541 gttgttgatc tgctttttca tgggtatgcc caagtcgcgc tcgaagatcg cggcgatgcg
    15601 gtgcgacaac gaaccgccgc ggataagctc ggccgagttg cgcatttcca aaatcgccga
    15661 ggcggcgatc gcttccagcc aagacttctg cgccaggtga atccaggcgc tgaaacaaac
    15721 ttcgccgccc tccatgcgcg cggtggtctc gaaggcttcg gcgctcaaac cgaacacttc
    15781 gccttctttg accgccaagg taatatgatc gaccttgcct tgggcctttt gcccgatcaa
    15841 ttcgtcttct tcatggcgcc agacaatctg cttcaccggc agcggcgccg aaccctgcac
    15901 atagccccag cagtcgcgtc gattctgcac atagtaagcc atctggatcg agtattgctg
    15961 cacccgcggc ttggtaaatt tactttgtaa cagtcgcttg aacggctggg actcgaactg
    16021 ctcattgcac agccgcacca attcctgcca catctgctct aaacccgtcg aatgcacgct
    16081 catgtttaac tcccgaatat caatttattt aacacaagcg agtttccggt gtccaaccaa
    16141 tgacggccgc gtcaatccgt gtagtcacga tcaggtaccg aagtacgcgc caacgccatt
    16201 atcgaacgct catactccaa acgccaaact tttgatcacc accggcacga aaccctcttc
    16261 cggcacgact ccgccgacat cgatgaaatc caattcggac agtgacactt cgtcgatgca
    16321 aggcatgctc catttcgccg acagcatgcc gccgatcacc tgaccgacgt tttgaccgaa
    16381 gaccaaa
//
LOCUS       DASQKS010000182         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_517359, whole genome shotgun sequence.
ACCESSION   DASQKS010000182 DASQKS010000000
VERSION     DASQKS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517359"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VE170_04705"
     CDS             complement(<1..568)
                     /locus_tag="VE170_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14765.1"
                     /translation="MGVAAVLWIIFAVAVAPSDLGAQSAAKKVRLGIQSNNIGFLPFH
                     LAYHKGFYREQGIDLETIFMATQAVNAAFVRGDIDYSAAINGVIQTIVRGNPAKILAV
                     AVDRPLISLIGRKEIRAAPDLKGKKIGGSTPGGTATLMADAALKHLGFQAGRDVTIVP
                     LRENRLAALESGAVDAALLGVPENILAVD"
     gene            complement(598..1698)
                     /locus_tag="VE170_04710"
     CDS             complement(598..1698)
                     /locus_tag="VE170_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14766.1"
                     /translation="MDSVKKNLVIDADAHVVECLRTWDFMDKSEQQYRPVPLETREEA
                     GVRQQFWVIDGKVRGFRFPAFSDDELDKRQQQVGRKFADQQASRELGNVDLRLEYMDK
                     TGVDVQVLHNTIFIESCADRPAADVALCKSWNRWLADIWKQSNGRLRWSCMPPLLSMP
                     DALDQIRWSRQNGAVAVCLRPVEGNRLLADPFFYPVYEEAQKLDMAIAIHIANGSPWL
                     NDLYNHPTRIAATLHRFRVPTVASFNDILLSEIHEQFPKLRFGFIEASAQWLPWVMHE
                     AKNRFKTMGRKLPDNLLKEYGMFVTCENSDDLPYIVREAGEDNLVIGTDYGHTDISSD
                     VDAIRIFRQRSDLTPAVKEKILSDNSRALYAL"
     gene            complement(1754..2290)
                     /locus_tag="VE170_04715"
     CDS             complement(1754..2290)
                     /locus_tag="VE170_04715"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ14767.1"
                     /translation="MSVMFVDIRGYTSLVEQMEPGAVATRLNRFYEIATRAIFRHDGT
                     LDKLVGDEVMAFFGAPLNRRDHPQRAVSAALEILRDVAALPESDRLEIGIGISTGPAF
                     VGNVGGQDFTDYTVLGDTVNVAARLQGAAAPSEILLGQGTFDPVASKFPQATRRELEL
                     KGKTEPVVAWEIARGIYD"
BASE COUNT          471 a          694 c          755 g          509 t
ORIGIN      
        1 ggtcaacggc gagaatattt tccggcacgc cgaggagcgc cgcgtcgacg gcgccagatt
       61 ccagcgcggc caggcggttt tcccgcagcg gtacgatggt gacatcgcgg cccgcctgaa
      121 agcccaaatg cttgagcgcc gcgtcggcca tcaaggtcgc ggtgccgccc ggcgtgctgc
      181 cgccgatttt cttgcccttt aaatccggcg ccgcgcggat ttccttgcgc ccgattagcg
      241 aaatcaacgg gcgatcgacg gcaacagcga gaatcttggc cgggttgccg cgcacgatgg
      301 tctgaatcac cccattgatg gcggcgctgt agtcgatatc accgcgcacg aaggcggcgt
      361 tgaccgcctg ggtcgccatg aaaatggttt ccaggtcgat gccctgctcg cggtagaagc
      421 ctttatgata ggcgagatga aacggcagaa agccgatgtt gttggactgt atgccgaggc
      481 ggactttttt cgcagcgctc tgcgcgccga gatcgctcgg agcgacggcg acagcgaaaa
      541 ttatccagag aacggcggca acgcccattg ttcctgtccc ggtgaattat gccgatctta
      601 tagagcgtag agcgcgcgcg agttgtcgct caagatcttt tctttgaccg ccggcgtcag
      661 gtcactgcgc tggcgaaaaa ttcttatcgc gtcgacgtcg ctggagatat cggtatggcc
      721 gtagtcggtg ccgatgacca gattgtcctc gccggcctcg cgcacgatgt agggaaggtc
      781 atcggagttt tcgcaggtga caaacatgcc gtactccttg agcaagttgt cgggcaactt
      841 gcggcccatg gttttgaagc ggttcttggc ttcgtgcatc acccagggca gccactgggc
      901 gctggcttcg atgaagccga agcgcagctt gggaaactgc tcgtgaatct cgctgagcag
      961 gatgtcgttg aacgacgcca ccgtcggcac gcggaaacgg tgcagcgttg cggcgatgcg
     1021 ggtcggatga ttgtagaggt cgttcagcca tgggctgccg ttggcaatat gaatagcgat
     1081 cgccatatcg agcttctgcg cctcttcata cacggggtaa aaaaacggat cggcgagcaa
     1141 gcgattgcct tccacgggac gcaggcagac ggcgacggcg ccgttttgtc gggaccagcg
     1201 gatttgatcc agcgcatccg gcatgctgag caacggcggc atgcaggacc agcgcagccg
     1261 gccgttgctt tgtttccaga tgtcggcgag ccagcggttc caacttttgc acagcgccac
     1321 gtcggcggcc ggccgatcgg cgcaggattc gatgaaaatg gtgttgtgca agacctgaac
     1381 atcgacaccg gtcttgtcca tatattccaa gcgcaaatcg acgttgccga gttcgcggga
     1441 ggcttgttgg tcggcaaact tgcggccgac ctgttgttgg cgtttgtcca gttcgtcgtc
     1501 cgagaacgca ggaaagcgaa agccgcggac tttaccgtca atgacccaga actgctggcg
     1561 cacaccggcc tcttctctag tctccagcgg caccgggcga tactgctgct cggacttgtc
     1621 catgaagtcc caggtgcgta agcattcgac gacgtgggca tcggcatcga tgaccaaatt
     1681 cttttttacg ctatccatgt ttgcctccgg ctgaaattcg atagcctaac ctaattgacc
     1741 ggatgagtgg ctgtcaatca tagataccac gcgcgatttc ccaagcgacc accggttcgg
     1801 tcttgccttt gagttctaac tctcggcgcg tcgcctgggg aaacttacta gccaccggat
     1861 cgaaggttcc ttgccccagc aatatctcac tgggcgcggc ggcgccctgc agccgcgcgg
     1921 cgacgttcac ggtgtcgccg aggaccgtgt aatcggtgaa gtcctggccg ccgacattgc
     1981 cgacaaacgc cgggccggtg ctaatgccga tgccgatctc gagtcggtcg ctctccggca
     2041 atgcggcaac atcgcgcagg atttccaatg cggcgctaac ggcgcgctgt ggatgatcgc
     2101 gccgattcag cggcgcgccg aagaacgcca tgacttcgtc gccgaccagc ttgtcgaggg
     2161 tgccgtcatg gcgaaagatc gcccgcgtgg cgatttcgta gaagcgattg agccgcgtcg
     2221 ccactgcccc tggctccatt tgctctacca gcgatgtgta gccgcggatg tcgacgaaca
     2281 tgaccgagat gtttttggtc tccccgcccg gaggagcgag ctcgtatcag agcgtgcaaa
     2341 ggtggggatt ttttcgtgac gggcgaatct gcagcatctg gtagggtaaa gcgaagatgc
     2401 ctagaaatgg cacgaggcat tctttgcag
//
LOCUS       DASQKS010000183         5020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3037942, whole genome shotgun sequence.
ACCESSION   DASQKS010000183 DASQKS010000000
VERSION     DASQKS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037942"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1166
                     /locus_tag="VE170_04720"
     CDS             96..1166
                     /locus_tag="VE170_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYJ14768.1"
                     /translation="MNRTWLVTAFFFALLLLILYGVYLIISPFITAITWAVILAILVY
                     PLYAWLLEVLRGRANLAAVIVIFSSTLLVIAPGFEAAWYLSDEAVELVHTVRALLSEE
                     GLKEWGQKPWVQQLQGWWSMVSFRLIDFKIDWKGGLVQGAQASSSFLVGQVKGIAQNV
                     LVFTANCIVALFTLFFLLRDGADFLRRLQRLLPMDQVHQERLLKNVVDAVIAVVHGSI
                     VVATVQGLLAGLGYWAAGVPFSVLWGVVTAFVSLLPVGGSTLVSIPAAIYLFLQGETV
                     RGIILLVWCLGFVGMIDNILKPLLIGNRLGLPVLLLFFGILGGLSLFGAVGIIIGPAL
                     FALLRALLDLYSQEYATPINDS"
     gene            1382..1627
                     /locus_tag="VE170_04725"
     CDS             1382..1627
                     /locus_tag="VE170_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYJ14769.1"
                     /translation="MTVDKEFFNVKYKEGSLDAKTAQLIFFAVCISIGHEEGATLHLK
                     RARECGASEDEITEALVYAMRPAAAKVRNFAKAIIAK"
     gene            1664..2518
                     /gene="trxA"
                     /locus_tag="VE170_04730"
     CDS             1664..2518
                     /gene="trxA"
                     /locus_tag="VE170_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYJ14770.1"
                     /translation="MSQWTIDVNEENFEAAVLERSSEVPVLVDFWAEWCGPCKALGPV
                     LEKLADEYAGGFVLAKLNVDENPNLAGMFGIQGIPAVKLFRDGELASEFTGALPEPML
                     REFLTKFLPTAADQEAQAAMALEAEGKTPEAKSAYEAILAADASHGLALLGMARIAMT
                     GGDNAAAREYLDRIALVADERKEAERMIARLDLQAGGAQNEAVLREKIAADPNNIEAR
                     FDLAQSLASVEKYDEALSEFLQVVKADRKFKDDGARKAMIQIFEVLGSDDPLTDKYRS
                     ELAAVLFR"
     gene            2593..3060
                     /locus_tag="VE170_04735"
     CDS             2593..3060
                     /locus_tag="VE170_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYJ14771.1"
                     /translation="MSMITLAQANRIIEAILARGRELTCRPMSVIVVEPGAKVKAFQK
                     EEDSAMMRFEMAYGKAYAALALGRSSSLVRMRTEERPLFMQYLMRASDDQIFAEGGGM
                     LIRDQAGDVIGAVGVTGDTQERDEELAAHGIRAAGLMTDEDCANLGMRVRLKN"
     gene            complement(3120..4805)
                     /locus_tag="VE170_04740"
     CDS             complement(3120..4805)
                     /locus_tag="VE170_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010441595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ14772.1"
                     /translation="MTDDSMTGAEAAVTMLKLHGVEFIFGLCGDTSLPFYDALARLPH
                     GMRHILARDERSAAYMADGFARVSGKVGVCEGPSGGGVTYILPGLAEANESSVPLLCI
                     NSDISVGARGRYTLTELDQGALMRPVTKWNAVLDRAADLPQIFRQAFKQITSNRPGAA
                     HIALPFDVQNGLVARSEVYGDASFGSCPAERIAPEAAAVEAAARLLTQAGTPVFICGG
                     GVVISQAEAELVELAERLSAPVATTISGKCSIDEQHGLAVGVVGSNGGTPETRAIVDQ
                     ADLIFFIGCRAGSVTTERWRHPAKGSTKVIHLDVDAAVPGTNYPTDAALVGDAKLGLK
                     MLSDALVTSHRPLDAAQVEAAKERKFAKFQALAESNDTPVKPERVVAELTRALEPDAI
                     VVADAGTPCPYFSAYFPVRGNGRKFFSNRAHGALGYSMAAAMGAHFAQPKVKCVAVMG
                     DGSFGFTCGELETAARYRLPVTFIVIANASYGWIKAGQRAGYGERYFAVDFAVTDHAA
                     VAAAFGVKSWRVTEPSELTKILKEALAHGGPSLVDIVCQPLHEAKAPVSEWIA"
BASE COUNT         1018 a         1389 c         1558 g         1055 t
ORIGIN      
        1 aagatcgatc ctttgcccgc gccgccggcg gcggctggca atgttgcagc tccgagcgct
       61 tctacgtaca gttggtgcat tgaaatggag gggcagtgaa ccgaacctgg ctcgtgaccg
      121 cttttttctt tgcgttgctg ctgctcattc tctatggcgt ctatttaatc atcagtccat
      181 ttatcacggc gatcacctgg gcggtgatct tggcgatcct agtctatcca ctctacgctt
      241 ggctgctgga ggttctccgt gggcgggcca acctcgccgc ggtgatcgtc attttctcga
      301 gtaccctgtt ggtgatcgcg ccgggattcg aggcggcgtg gtatctctcc gatgaggcgg
      361 ttgagctcgt gcacaccgtg cgggcgctgc tcagcgagga ggggctcaaa gagtgggggc
      421 agaagccttg ggttcaacag ctacagggtt ggtggagtat ggtgtcgttc cgtttgatcg
      481 atttcaaaat cgattggaag ggagggctgg tccagggcgc gcaagcgtcg tcgtcctttc
      541 tcgtcggaca ggtcaaaggc atcgcacaga acgttttagt gttcacggcc aattgcatcg
      601 tcgcgttgtt tacgctgttt ttcttgctcc gcgacggtgc cgattttttg cggcgcttgc
      661 agcgcctgct gcccatggac caggtgcatc aggaacgctt gctgaaaaat gtcgtcgatg
      721 cggtgattgc cgtggttcat ggctcaatcg tcgtggccac tgtccaaggc ttactcgccg
      781 gcttgggtta ttgggccgcc ggtgtgccct tctcggtgct ctggggcgtg gttaccgcgt
      841 tcgtttcgct gttgcccgtg ggcggctcga ccctggtgtc cattccggca gcgatttatc
      901 tatttctcca gggcgaaacc gtcagaggca tcattttgtt ggtctggtgt ttaggttttg
      961 tcggcatgat cgacaatatc ttgaaacctt tgctgatcgg caatcgtttg ggcttgccgg
     1021 tgttgttgct cttcttcggc atcctcggcg gcctctcgtt gttcggcgcc gtgggcatca
     1081 ttatcggacc ggcgctgttc gcgttgttgc gcgcgctctt ggatctctat tcccaagaat
     1141 acgcaacgcc gataaacgat agttaggagt taggcgcggg cggttggatt ctctaggtga
     1201 atccgaatat attgaccgcg aatcagccgg gcaatagcac cgaaggagta tccagggccg
     1261 acccacggca caagcattcg ccgcattgaa cgcaaacgag aatgtgattt catagggatg
     1321 gtttgaaaac gccgtccggg ttgcaattct aacgttgtca gcaaaaaaat agggaggaga
     1381 aatgaccgtc gacaaagaat ttttcaacgt gaagtacaaa gagggttccc tcgatgcaaa
     1441 aaccgcgcag ctgatatttt ttgcggtctg tatctcgatc ggccatgagg agggcgccac
     1501 tttgcatttg aaaagagctc gcgagtgtgg cgcgtcggag gatgagataa ctgaagcgct
     1561 ggtttacgcg atgcgtccgg cggcggccaa ggtgcgcaac ttcgccaagg cgattatcgc
     1621 aaaataaatt gattcaaagt tccaaaaaag tgggtgacac aaaatgagcc agtggaccat
     1681 cgacgttaac gaggaaaatt ttgaggcggc ggtgctggaa cgctcgagcg aagtgccggt
     1741 gctagttgat ttctgggcgg agtggtgcgg gccgtgcaaa gcgctggggc cggtattgga
     1801 aaagttggcc gacgaatacg ccggcggctt cgttctggcc aaactcaatg tcgacgagaa
     1861 ccccaatctc gcggggatgt tcggcatcca gggcattccc gcggtgaaac tatttcgcga
     1921 tggtgagctc gcctcggaat tcaccggcgc cttgcccgag ccgatgctgc gcgagttcct
     1981 caccaagttt ttgcccacag ccgccgatca ggaagcgcag gcggccatgg cacttgaagc
     2041 cgaagggaaa actcccgaag ccaaatccgc ctatgaagcg atcttggccg ccgacgctag
     2101 tcatggcctg gcgctcttgg gcatggctcg catagccatg accggcggcg ataacgccgc
     2161 agcgcgggaa tatctcgaca ggattgcgct cgtcgccgac gagcgcaagg aagcggagcg
     2221 catgatcgct cgtctcgatc ttcaggcggg cggcgcgcaa aacgaggcgg ttcttaggga
     2281 aaaaattgcc gcggatccga acaatatcga ggcgcgtttc gatctcgcgc aatcgctggc
     2341 cagcgtcgaa aaatacgatg aggcgctcag cgaatttctc caagtcgtca aggccgaccg
     2401 gaagttcaaa gacgacggcg cgcgcaaggc gatgatccaa atcttcgagg tgctgggctc
     2461 ggacgatccc ttgacagata agtatcgttc ggagctggcg gccgtgctgt ttcgctgatt
     2521 aaattcatgc cgcttcatcg gggaagcccg acgatttcgc cggcgatggc acgagatcag
     2581 tgaggaggtg ctatgagcat gatcacgttg gcgcaagcca atcgcattat cgaggcgatc
     2641 ctagcgcggg gccgtgaatt aacttgccgg ccgatgagtg tgatcgtggt cgagcccggc
     2701 gccaaggtca aggcatttca gaaagaagaa gactcggcga tgatgcgttt cgaaatggcc
     2761 tacggcaaag cttacgcggc gctggcgctt ggacgctcgt catcgctcgt gcgcatgcgc
     2821 acggaagagc ggccgttgtt catgcagtat ctcatgcgcg cctcggacga tcagattttt
     2881 gccgagggcg gcggcatgct gatccgcgat caagcgggag atgtcatcgg tgccgtcggc
     2941 gtcaccggcg acacgcaaga gcgcgacgaa gaactcgccg cccacggcat tcgagctgcc
     3001 ggtctgatga ccgatgagga ctgcgcgaat ctgggaatga gagtgcggct gaagaactaa
     3061 gaatctgccg caaagaacgc aaaggtcgca aaaaaaaaag gattaccgcc aacgggactt
     3121 cacgcgatcc actccgagac cggcgctttg gcttcgtgca gcggctgaca gacaatatcg
     3181 acgagactcg gtccgccgtg agctaacgct tctttcaata tcttggtcaa ttcactcggt
     3241 tcggtgacgc gccaactctt gacgccgaaa gccgcagcga cggccgcgtg atcggtaacg
     3301 gcgaagtcga ccgcgaagta acgctcgccg tagccggcgc gttgccccgc cttgatccaa
     3361 ccgtagctgg cgttggcgat gacgataaac gtcaccggca gccgatagcg cgcggcggtc
     3421 tcgagttcgc cgcaggtaaa tccgaagctg ccgtcgccca tcacggcaac gcatttcact
     3481 ttgggctgag cgaaatgcgc gcccatcgct gccgccatcg agtagccaag ggcgccgtgg
     3541 gctcggttag aaaaaaattt tcgcccgttg ccgcgaaccg gaaagtaggc ggagaaatac
     3601 ggacaaggag tgccggcatc ggcgaccacg atggcatccg gttcgagcgc gcgagtcagt
     3661 tcggcgacca cgcgctcggg tttgaccggc gtgtcgttcg attccgccag tgcttgaaat
     3721 ttcgcaaact tccgttcttt cgctgcttcg acttgcgccg cgtcaagggg acgatggctg
     3781 gtcaccagcg cgtcacttaa cattttcaac ccgagcttgg catcgccgac cagcgcggcg
     3841 tctgtcggat aattggtgcc gggaaccgcg gcatcgacat cgagatgaat gactttggta
     3901 ctgccctttg cgggatgacg ccaacgttca gtggtcaccg agccggcgcg gcagccaatg
     3961 aaaaatatca gatcggcttg atcgacaatc gcccgcgtct caggcgtgcc gccgttggag
     4021 ccgaccacac cgacggcgag cccgtgctgt tcgtcgattg agcatttgcc gctgatggta
     4081 gtcgccaccg gcgctgaaag ccgctcggcg agttcgacaa gctccgcctc ggcttgcgag
     4141 atcacgaccc cgccgccgca gataaagacc ggagtacccg cttgtgtgag taagcgcgcc
     4201 gcggcttcta ccgcggccgc ctcgggcgca attcgctccg ccggacagct gccgaaactc
     4261 gcatcgccgt aaacctcgct tcgcgccacc aagccatttt gtacgtcgaa gggcagagcg
     4321 atatgggccg cgccaggtcg gttcgaagtg atttgcttga acgcttggcg aaaaatttgc
     4381 ggtaggtctg cggcgcggtc gagcaccgca ttccatttcg tcacggggcg catgagcgcg
     4441 ccctgatcca gttccgtcag cgtgtaacga ccgcgcgcgc cgaccgagat atcgctgttg
     4501 atgcacaaga gcggcacgga agattcgttg gcttccgcca gacccggtaa gatataagtg
     4561 acgcccccgc cgctcggtcc ttcgcacacg ccgactttgc cggacacgcg cgcaaaaccg
     4621 tcggccatgt aggcggcgga gcgctcgtcg cgcgcgagga tatggcgcat gccgtggggc
     4681 aggcgcgcca gggcgtcata aaacggcaga ctggtgtcgc cgcacaggcc gaaaataaat
     4741 tcgacgccgt gcagcttgag catggtcacc gcggcttcgg cgccggtcat ggaatcgtct
     4801 gtcatcgatg cgcgcagcgt aacagggtcg cgcggccctg acaacgcttt tcatgtcggt
     4861 tcaatatggc tttgtgatac atttaaacgc ggcgaatatg gcgagcgtgc gaaccgaaca
     4921 agaaatccag aaactggcgc gcactcttta tcgacgagcg gcgcagcata agccgtcgtt
     4981 gttcgacgca caggatttgc tcggccggat gatcgaacgg
//
LOCUS       DASQKS010000184         4234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_956843, whole genome shotgun sequence.
ACCESSION   DASQKS010000184 DASQKS010000000
VERSION     DASQKS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956843"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1403)
                     /gene="amrB"
                     /locus_tag="VE170_04745"
     CDS             complement(135..1403)
                     /gene="amrB"
                     /locus_tag="VE170_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HYJ14773.1"
                     /translation="MDDKPRVRAIEAFPVQQDGKTLIYLKDPLNFATPLGISPVGYFL
                     LAHFDGRHAYSDLREIFEQRFRAPLAHDEFEQFVGMLDQHYFLKSPRFEHYQQAVIAE
                     FRARPSRAPAHVVSGYKSDPAALTAQLDGYFTAPTGPGVPSVSNGAFLPTAPKAPKAI
                     VAPHIDFHRGGPAYAWAYKPLAESAGADLYIILGTSHCGGQTPYILTRKSFETPLGLV
                     ETDGEFVGQLQSQCAEDCFTDEYLHRGEHSIEFQVLFLKYVAQKRAALTGAPEKPFKI
                     VPVLVSSFHSMMVSLTPPEETPAVGCFLRTLRQLAQQEARQVCFVAGVDLAHVGKQFG
                     DEEPVTGDFLKWVEAEDHKLVDRLSALDAPGFFHEIAKDEDKRKICGFAPLYSLIHLL
                     DGVQGQHLHYGQAFTPETGSAVTFTSLIFP"
     gene            complement(1444..1743)
                     /locus_tag="VE170_04750"
     CDS             complement(1444..1743)
                     /locus_tag="VE170_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYJ14774.1"
                     /translation="MITKEQVYEALHECFDPEIPVNIVDLGLVYDVEVTDDKVAVKMT
                     LTAPGCGMGGMIAADAKEKIMAIEGVSDATVDLVWEPAWNPSMIAEEAKQRLGIV"
     gene            complement(1781..2884)
                     /locus_tag="VE170_04755"
     CDS             complement(1781..2884)
                     /locus_tag="VE170_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HYJ14775.1"
                     /translation="MENISQEQIIGALRQVQDPELHRDIVSLGMVKDLAIDGGRVRFT
                     VELTTPACPLRETIETDCKKALGAVPGITGLEINFGAQVRGSKSGAGQTDLLPTVRNV
                     VLVAAGKGGVGKSTVAANLAVALKMLGAKTGLLDADIYGPSVPIIMGVKGEPRKIDVN
                     GVQKIEPTMAHGVPVMSIGFFLDADQAVIWRGPMLGKALHQLMGDVHWGELDYLVVDM
                     PPGTGDVQITFSQQLKVSGALLVATPQHVALADVVRAKSMFDKVMIPIVGLVENMSYF
                     ICDGCNKEHDIFSRGGAKQAAERFQIPFLGEIPITPALRQGGDEGVPILIQDPNSPVS
                     KSFLEIAGKLAGQLSIAAERGNKGQGPTIITTH"
     gene            3325..4038
                     /locus_tag="VE170_04760"
     CDS             3325..4038
                     /locus_tag="VE170_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ14776.1"
                     /translation="MKNIHRRTTAPFALLVAAFLSGCAGGDLTTREKGAGIGALGGAA
                     AGGLIGAAVGRPGVGAAVGGALGLGTGALIGDQLQGQGNRQNEQQKAIDQQRVELEKN
                     RALIEELKKNKIEAKETSRGVMVNLPSVNFEFDSADLSRDGRERVNQIADIIRRDAPN
                     RRVTVEGHASRESPAAEAYNQRLSERRASTVADSLERNGVNGRNISSQGVGTRAPVAS
                     NDTESGRQQNRRVEVIIEN"
     gene            complement(4035..>4234)
                     /locus_tag="VE170_04765"
     CDS             complement(4035..>4234)
                     /locus_tag="VE170_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011130769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HYJ14777.1"
                     /translation="TRLVSLSSIVAAALAPFALWLFLQPPPIVLLGAFLAAMIVWRHR
                     GNIQRLLAGTEPRFDARSGAD"
BASE COUNT          967 a         1234 c         1190 g          843 t
ORIGIN      
        1 cagtgcctac cattctgtcg cactgctaaa ttgccgagcc accgcagcgc ctcaacgcag
       61 ccacggcgta gatcgaaatg aaacaaaaag cggataaggg agtgggaaca ctactcgccc
      121 ggtttggccg ccgatcacgg aaagatcaga ctggtaaacg tcaccgccga gccggtctct
      181 ggcgtaaatg cctggccata gtggagatgc tgaccttgca caccatcgag caggtgaatc
      241 agtgaataga gcggtgcgaa gccgcatatc ttgcgcttgt cctcgtcttt ggcaatctcg
      301 tgaaaaaaac ccggcgcatc caatgcgctt aaacgatcga ccagcttgtg gtcttccgcc
      361 tctacccatt ttagaaaatc gccggtgacc ggctcctcgt caccgaactg tttaccgaca
      421 tgagccaggt cgacgccggc gacgaagcaa acttgccgcg cctcctgttg cgctaactgg
      481 cgcagcgttc gcaagaagca accaaccgcg ggagtctctt ccggcggcgt cagactgacc
      541 atcatcgagt gaaacgacga aaccaagacc ggcacgatct taaacggttt ctccggtgcg
      601 cccgtaagcg ccgcgcgttt ctgcgcaacg tacttcaaga agagcacctg aaattcgatc
      661 gaatgctcgc cgcgatggag atattcgtcg gtgaaacaat cctcggcgca ctgcgattgt
      721 aactgcccga cgaactcacc gtcagtctcg accaaaccga gaggcgtttc aaagctttta
      781 cgggtgagaa tgtatggcgt ctggccgcca caatgtgatg tgccaaggat tatatagaga
      841 tcggcgccgg cactttcagc caagggcttg tacgcccatg cgtaagctgg gccgccgcga
      901 tgaaaatcga tgtgcggcgc cacaattgct ttgggcgctt tgggcgccgt tggcagaaac
      961 gcgccgttcg atacggaggg cacgccggga cctgtcggtg cggtaaaata accgtcgagc
     1021 tgcgctgtca gtgccgccgg atcgctcttg taaccgctga cgacgtgggc cggcgcacgg
     1081 ctcggccgag cgcgaaactc ggcgatcacc gcttgctggt agtgttcaaa gcgcgggctt
     1141 ttcaaaaaat aatgctgatc gagcatgccg acgaattgtt cgaactcgtc atgcgccaac
     1201 ggcgcgcgaa agcgctgctc gaaaatttcg cgtagatcgc tgtaggcatg gcggccatcg
     1261 aaatgggcca aaagaaaata acccaccgga gagattccca ggggcgtggc gaaattgagc
     1321 gggtctttaa gatagataag agttttgccg tcttgttgga cgggaaacgc ttcgatggcg
     1381 cgaactcttg gtttgtcgtc catggagaca aagtcggctg ggaacgcggg cgcgaccgat
     1441 gatttagacg atgcctaaac gctgcttggc ttcctcggcg atcatgctcg gattccacgc
     1501 cggctcccaa accagatcga cggtggcatc gctgacccct tcgatggcca tgattttctc
     1561 tttggcatcc gccgcgatca tcccgcccat gccgcaaccc ggcgcggtca gcgtcatctt
     1621 caccgccact ttgtcgtcgg tgacctcgac atcgtaaacc aatccgaggt cgacaatgtt
     1681 gacgggaatc tccggatcga aacattcgtg caaggcttca taaacctgtt ctttggtaat
     1741 catactagtt ctcctcttgg cgtgctggcc gacacacgga tcaatgcgtg gtaataatcg
     1801 tcggcccctg tcccttattg ccccgttcgg cggcgatcga caactgcccg gccaatttgc
     1861 cggcgatttc gagaaaactc ttgctcaccg gcgagtttgg atcctgaatc aagatcggca
     1921 cgccctcgtc gccgccttgg cgcagcgccg gggtgatcgg aatttcgccg agaaagggaa
     1981 tctgaaagcg ttccgccgcc tgtttggcgc cgccgcggga aaaaatatca tgttccttgt
     2041 tgcagccatc gcagatgaaa tagctcatat tctcgaccag gccgacgatc ggaatcatca
     2101 ccttgtcgaa catcgatttc gctcgcacga catcggccaa ggcgacatgt tgcggtgttg
     2161 ccaccaacaa ggcgccgctg accttgagct gttgcgaaaa agtaatttgc acatcaccgg
     2221 tacccggcgg catatcgacg acgagatagt ccagctcgcc ccagtgcacg tcgcccatca
     2281 gttgatgcaa cgccttgccg agcatcggcc cgcgccagat caccgcctga tcggcatcga
     2341 gaaaaaaacc gatagacatc accgggacgc catgggccat cgtcggttcg atcttttgca
     2401 caccgttgac gtcgattttt cttggttcgc ctttaacgcc catgatgatc ggcaccgatg
     2461 gcccgtagat atcggcgtcg agcagcccgg tcttggcacc gagcattttc agcgccacag
     2521 ccagattagc cgcaaccgtc gatttgccga cgccgccctt tccggccgcc accaggacga
     2581 cattcctaac cgtcggcaac aaatcggttt gcccggcgcc ggatttgctg ccacgcactt
     2641 gggcaccgaa gtttatttcc aggccagtga tgccggggac cgcgcccaaa gcttttttac
     2701 agtcggtctc aatggtttca cgcagcggac aggccggcgt ggtcaattct acggtgaagc
     2761 gcactcgacc gccgtcgatg gcgaggtctt tgaccatgcc gagactgacg atgtcgcggt
     2821 gcagttccgg atcttggacc tggcgcaacg ctccaatgat ttgttcttgc gaaatatttt
     2881 ccacgaaatt cctctcgacg cgaacgaact atagacttat aaggtctatg aggtcaatga
     2941 cgcgagcgca tatccagtca aactcgccgt tggtgtcggc gaatcatttc acaagccaat
     3001 tgaatcgctc cctgttcctt gccgcattca ccgctctttg gtaatattta ccgatagttg
     3061 ccgccagttc accggattct ctcgctcgca gatcaatgaa taagccggat atggcccatt
     3121 ttggcgccag atcgacaacg ccaatcgggg aattgagcca aagtcgcgaa agcgacaaga
     3181 aacaccgcca acgcgaatac tgagcggcgc tgaccgtaac tgctgacagc aatagggatt
     3241 tccccattgc cgttatctat ttactgtagg actacgtcta accagagacg gccgatgcta
     3301 catagtgaag gccgaggagg aattatgaaa aatatccatc gacgaaccac tgctccattt
     3361 gcactcttgg tcgccgcatt cctgtccggc tgcgccggag gtgatttgac gacccgcgaa
     3421 aaaggtgccg gcatcggcgc cttgggcggc gccgccgcgg gtggcttgat cggagctgcc
     3481 gtgggccggc ccggagtcgg agctgccgtg ggcggcgcgt tgggcctggg cacgggcgca
     3541 ttgatcggcg accagctgca aggccaggga aaccgtcaaa atgaacaaca gaaagcgatc
     3601 gaccagcaaa gagtcgaact cgagaagaat cgcgcgctga ttgaagaact gaaaaaaaat
     3661 aaaatcgaag cgaaggaaac cagccgcggc gtcatggtca atttgccgag cgtcaatttt
     3721 gaatttgaca gcgccgatct ttcgcgcgat ggccgcgagc gggtcaacca gatcgccgat
     3781 attattcgcc gcgatgcgcc caatcgccgc gtcaccgtcg aaggccacgc cagccgggag
     3841 agccccgccg ccgaagctta taaccagcga ttgtcggaac ggcgcgcctc taccgtagcc
     3901 gatagcttgg agagaaatgg tgtgaacggc aggaacattt cgtcccaggg cgtcggcacc
     3961 cgcgccccgg tggcgagcaa cgataccgaa agcgggcggc agcagaatcg ccgcgtcgaa
     4021 gtgattatcg aaaattaatc agcacccgag cgcgcgtcaa agcgcggctc ggtgcccgcc
     4081 agtaaacgct gaatattgcc gcgatgacgc cagacgatca tcgcggcaag aaaggcgccc
     4141 agcaagacga tcggtggtgg ctgtaaaaac agccagagcg cgaatggggc caaagccgct
     4201 gcgacgatcg aactcaacga caccagccgg gtag
//
LOCUS       DASQKS010000185        12764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4717942, whole genome shotgun sequence.
ACCESSION   DASQKS010000185 DASQKS010000000
VERSION     DASQKS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4717942"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VE170_04770"
     CDS             complement(<1..350)
                     /locus_tag="VE170_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HYJ14778.1"
                     /translation="MELVALVIFSYLLGSIPTGFIVAKLAGVDIRQVGSGNVGATNVA
                     RAVGKRQGALTLLADTLKGLVPTAIGLAMTLPAAALAAIPAAAFLGHLYPVFLKFRGG
                     KGVATALGALLVIAP"
     gene            complement(372..782)
                     /locus_tag="VE170_04775"
     CDS             complement(372..782)
                     /locus_tag="VE170_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14779.1"
                     /translation="MTFKNGHSGTLEKAEKPPGEWFLIAAAVDGHDGYVPGRFAKLVE
                     NAPSPLPSAPAGPAITKTEKATSGATAAAPAQVARAEPADGKSPSILQLLEVHEKEVK
                     VALAVAGVAFLLGWLCGGMYSVRRERKHRRRLRL"
     gene            complement(1079..2170)
                     /locus_tag="VE170_04780"
     CDS             complement(1079..2170)
                     /locus_tag="VE170_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HYJ14780.1"
                     /translation="MSKPAVQSENVMDNIPFYDKWQLGEGIPIMKTFFVQDLKKVDLK
                     PWARTGGSGAFINMEGAEGATGAYVSEIPAGKKLNPVRHLYEDLHFVLKGRGATTIWN
                     DKGVKTQFEWHDGSLFALPLNCTYQHFNGSGDEPARFFSVNSAPIVFNLFHHEDFVFN
                     TPRDFTDRFDGDPEYFSGKGKAYPGRVWDTNFIADARSFALEDWVERGGGGKNVMLEM
                     ANGSMAAHISEFPVGTYKKAHRHGPGFNVIIIKGKGFSMFWRQGEEPKRYDWQDGSVF
                     TPPAMMWHQHFNTGASGARYLPPRLGGIKYSLGEGFGDITKVDKDVKAGGNQIEYFDE
                     DANIRKMFEEELAKSGTTSKMDPNFYKRP"
     gene            2377..2592
                     /locus_tag="VE170_04785"
     CDS             2377..2592
                     /locus_tag="VE170_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HYJ14781.1"
                     /translation="MATSIEAPMVGKILKIEKQVGDHVEEDDVVVVMEAMKMEIPVVA
                     PAAGVVTEIKVSPGQAVEAEQILAVIE"
     gene            2644..2790
                     /locus_tag="VE170_04790"
     CDS             2644..2790
                     /locus_tag="VE170_04790"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HYJ14782.1"
                     /translation="MDTWTFGWTLALIGMGGTMLVLWLISVMILLIRKIFPYQAEPSA
                     AKKG"
     gene            2799..3947
                     /locus_tag="VE170_04795"
     CDS             2799..3947
                     /locus_tag="VE170_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HYJ14783.1"
                     /translation="MESALVSGIQGLFAGVVSITIWQIVMLIIASVLLYLGIKKGYEP
                     LLLVPIGFGAILVNIPLADMMGKDGFLRFFYDAGVLTEVFPLLIFIGIGSMTDFQPLL
                     QNPKIILLGAAGQFGIFLTLLLALALGFDKLDAVAVAIIGACDGPTAIYVSSKFAPHM
                     LGAVSVAAYSYMALVPLIQPPIMRALTTEKERQIVMGAAKLEPISKTTKIVFPIAVTI
                     FASILAPKGAPLIGTIMLGNLLRESGVVERLKSASENEIANIVTLLLGLCIGATMEAD
                     KFLKAQTLLILGLGFIAISLDTVVGLLFGKLMCAMTGGKVNPLIGAAGISAFPMAARV
                     VQAEGQKYNKKSYLLMHAMSANAGGQIGSVIAAAVMLSVLQGMGIVGG"
     gene            3961..5514
                     /locus_tag="VE170_04800"
     CDS             3961..5514
                     /locus_tag="VE170_04800"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HYJ14784.1"
                     /translation="MAKSREELEQKLAGLEQKVTAGDPKTAAKLHEQGNLTARERIDK
                     LLDPGTFVEEFMLAETPCMDFGMAERKQPSDGVVTGFGKIDHRPVYVYSQDRSIMAGS
                     VGAAHAEKIAYVIQSARKVGVPVIGLNDSAGARIQEGLSVTSAIGKIFFENSISSGCI
                     PQISAIMGPCIGVGSYSPALTDVILQVQNSGQMFITGPGVIKEVLGETVTMEELGGTK
                     IHSEISGVTDLVAKNDEECLAMIRRLVGFLPASFEEKPPRKIPTDDPGRMLDSLEKLV
                     PEDMKRAYNILQVIKAIVDDGDFFELKAKFARNLVIGFGRLDGYPVGLVANQPMFLAG
                     SLDVDASDKAARFIRFCDAFNIPVITLMDVPGFFPGKQQEEKGIIRHGAKMLYAYAEA
                     TVPKITIVLRKGYGGAKQALCTREMGADQLFVWPGVELAVMGGGGAVNVLYKKEIDQS
                     ADPAKTRTEKLAEYLERFSGPFEALSKQFAQAAIRPADTRKRLIQSLEILRNKKEERP
                     RKKHGLMPV"
     gene            complement(5511..8129)
                     /locus_tag="VE170_04805"
     CDS             complement(5511..8129)
                     /locus_tag="VE170_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ14785.1"
                     /translation="MAKLQIEKIVQLATEPFLRRLRRRLLLMIAIAAVPILAVVLYQA
                     KVHRDVQILEVHEAAWRLANIIALRQSQLVDAAKQLLMHLAQTPAIARVDRGPCEEIV
                     RGFLARNRAYLDIGVSDPAGAMRCRASDFGSSVDLARSTLVRRVMATKDFVIGDYQVL
                     GNWRRRALTFGYPILGTDGAPREILFAALDLKRVEQITAESNLPAGMTLTILDSHGTI
                     LTRLPDHEAWVGQRLPDAPHLEMIPLRRQTTKEIQGLDGITRLYAFKTVGDGREGGQI
                     NVIASVPKAIAYGEANQTLLWSLFWIAMLTASASSAAWLVGSKSVVEYVKKRSEADEA
                     RAKLAAIVESSEDGIIGMTLDGVISSWNDGAEQIYGYDAAAMTGQNISLLIPAEHRGE
                     IPELMQLVCLGKGINRYESERICKDGRRIIVSASLSPIRDGQGSVRGVATITRDITLL
                     RKGEEQMAMHTRQLETLQTVAQEIAETLSLAETMPQTLETLIARVPCDHAIIYCIGAD
                     GAADSYSASRGAEAQAPHRSLADELAPAASQCTGEWFVEDVSLVPELAALWPHHGVRA
                     AAILPITRKDRGRTTLVLLDNSSRSFAVGDIQFLKAMARQIALGVENGQLYRASLGLI
                     DNLAGEIEERRNAEKQLADFNAMVIHDLRSPLGNIVSMAESVKEGLFGAVNALQRTWL
                     GKIEKNCRGLIDQVSDFLDFSRIGAGKLVLKRERISIESQIHEIVTEHSIEAKKRNIR
                     LSARIAERLPVLWADPRRVGQVLTNILSNALKFTDNGGAVEVSARSSENAIVVSVRDT
                     GVGMAADELGDIFQMYRQGASSDKSQSRSTGIGLMICKRIVEAHGGRIWVESEPGKGS
                     NFYFSLPAEIEPEPAA"
     gene            complement(8216..8413)
                     /locus_tag="VE170_04810"
     CDS             complement(8216..8413)
                     /locus_tag="VE170_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ14786.1"
                     /translation="MERTNESLQLLTLTEAAELLHVSTKTVQRMIRSGDLPALKVGGQ
                     WRLRESQLISWVQSRESKSHS"
     gene            complement(8682..10613)
                     /locus_tag="VE170_04815"
     CDS             complement(8682..10613)
                     /locus_tag="VE170_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HYJ14787.1"
                     /translation="MARKRYLKKTPLSEARELFLSTVNPAALATEEIAIDDGLDRITA
                     EPVFAKISSPHYHASAMDGICVRAEETFGATEFVGKKLTLIDADSPRADGFRYVDTGN
                     ALPAWANAVIMIEKVHQVDATTIEIFESVAPWNHVRLVGEDVVATELLLPRSHRLRPY
                     DLGALLAAGHTTVRVKARPRVGIIPTGDELIQPGEEATPGAVIDFNSTVLAAFVREWG
                     GEPVKYPRARDDETLLRAALERAGAECDLVTIIAGSSAGEHDLTADVVAAAGELLAHG
                     IDVMPGKPAVLGSLAGKPVLGVPGYPVSAIVIAREILRPALEKFLSNAATTLPSVRAI
                     VPKKIASHLGLEEFIRVTLGRVGDKLVAVPLARGAGVITTMVHADGLMRIPSLVEGLN
                     AGEDAEVELLRPMVDVENTILCTGSHDLAIGVLEDQLKLRRPELKIAATNVGSLGGLL
                     ALQRGETHIAGTHLLDPASGVYNIPDIQRAIPNVAVALIHLAQREQGIIVSRGNPKAL
                     RGLPDLARRDLRFVNRQQGSGTRVLLDYQLKKLQLDGAAIAGYEREEFTHMAVGVAVA
                     SGLADAGLGVRAAAQALKLDFIPVANEQYDLLIDRRFFESPRGELLLATICSGGFKSA
                     VAALGGYDTTNAGALLYRQ"
     gene            complement(10664..11899)
                     /gene="glp"
                     /locus_tag="VE170_04820"
     CDS             complement(10664..11899)
                     /gene="glp"
                     /locus_tag="VE170_04820"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYJ14788.1"
                     /translation="MKDFFKVEPPQEARRILLEVLPVGTETVASVRARSRVLAEDLFS
                     QVDLPHFHRAAMDGYAVKAKDTFGASQSLPAYLKLAGVVEMGKEATQALDKGEAMRIS
                     TGGMMPPDADAVVMVEYTDETDGGLVEIHRAVSPGQNVIQIGDDIKRGEPVFAQGRRL
                     RGHDLGALTGVGIESITVYKRPRVALISTGDEIVDAAVDPQPGQVRNINQHSLAGLIE
                     EAGGELKDWGVIRDDRALLTAAIGAALDWADAVLLSGGSSMGAKDIALETILSFPDSQ
                     FIFHGISIAPGKPTIFAKACGKPILGLPGYPVSALVIFDLFGAPMLRRLGGESRDALQ
                     RFSHTVRAKLKTNIASQIGREDYVRVVMEGEGGSFVAAPLPSKSGAIFTLVKADGMVR
                     IGMNQDGLEQGEDVDVILF"
     gene            12150..>12764
                     /locus_tag="VE170_04825"
     CDS             12150..>12764
                     /locus_tag="VE170_04825"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HYJ14789.1"
                     /translation="MTTGRKDGAARATFMPQIVIASFNLATPEINVALMNDTPAGEAT
                     AVILAGGKSSRMGQPKALLLFAGEPLIVHLVRRLGQLFSDIVVVAAPEQDLPVLSATI
                     VRDDVAHQGPVGGIYYGLEAARREICFVASCDAPFLNLRLIAYLMTQISDFDVVVPFI
                     QDRYQPLHAVYRKQVAPLLKEQLERGELRPIFLYDKVRTRKVTEA"
BASE COUNT         2714 a         3823 c         3544 g         2683 t
ORIGIN      
        1 ggcgcaatga ccaagagcgc tcccagcgcc gtcgccaccc ctttgccgcc gcgaaacttc
       61 aaaaataccg ggtaaaggtg gccgagaaaa gccgcggccg ggatcgctgc gagcgccgcc
      121 gccggcagcg tcatcgccaa accgatcgct gtcggcacca gacctttcaa agtatctgcc
      181 agcaaagtta aggctccctg ccgtttgccg accgctcgcg cgacattggt cgcgccgaca
      241 ttgccgctgc ccacttggcg gatatccaca ccggcaagtt ttgccacaat aaatccagtt
      301 gggatcgagc cgagtaggta ggaaaaaata actagcgcga ctaattccat gattatcagg
      361 cgaccgtgcg actagagccg caggcgccgc cggtgtttgc gctcgcgccg gaccgagtac
      421 ataccgccac aaagccagcc caagagaaac gcaacgccgg caaccgctaa cgcgaccttc
      481 acttcctttt catggacttc gagcaattgt agtatggaag gcgatttccc gtccgcgggc
      541 tcggcgcggg ccacttgcgc aggcgcggcg gctgtcgctc cagatgtcgc cttttcagtc
      601 ttggtaatgg ctgggcccgc cggtgcagat ggcaatggcg agggagcgtt ctcgacaagc
      661 ttggcaaatc tcccgggaac ataaccgtcg tggccatcga cggcagcggc aatcaaaaac
      721 cactcgcccg gcggtttttc agctttttca agggtgcccg aatgtccgtt tttgaatgtc
      781 aatttggaac tatgtcccgc cgcggcacca tttgagttgg ccatcgcggt ttcagatatc
      841 gtgcctaatt ggcgaaccgg ttcggccgcg cgtgagtgat cggcgtgcac caagattcca
      901 atcacgacca cacacgagaa aaaatggcga cgactcgata acatcgactc aatctaacac
      961 aagcgccgat tgacgctcaa cgaattttct taatcgaatt tttctaaatc gccgcacaac
     1021 aaaaaagccc cgcagggaat acccggcggg gcttgtcagc gtaaaaaagc tccaaggctt
     1081 acggtctttt gtaaaaattc ggatccatct tgctggtcgt gccggatttg gcgagctctt
     1141 cctcgaacat cttgcggatg ttggcatcct catcgaagta ctcgatttga ttgccgcccg
     1201 ctttgacgtc cttatcgact ttggtgatgt cgccgaaacc ctcgcccaaa ctgtacttga
     1261 tcccgcccaa acgcggcggc agatagcgcg ccccgctggc gccggtgttg aagtgctggt
     1321 gccacatcat cgccggcggg gtaaacacgc tgccatcctg ccaatcgtaa cgtttcggct
     1381 cttcgccctg gcgccaaaac atcgaaaatc ctttgccctt gatgatgata acgttgaagc
     1441 cgggaccatg gcgatgggcc ttcttgtagg tgccgactgg aaattccgag atatgggcag
     1501 ccatggaacc attggccatt tccaacatga catttttgcc gccgccgccg cgctcgaccc
     1561 aatcttccag cgcaaagcta cgggcgtcgg caataaaatt cgtatcccag acccggcccg
     1621 gataggcttt gcccttgccg ctaaaatact ccggatcgcc gtcgaaacgg tcggtgaaat
     1681 cccgcggcgt gttgaagaca aagtcctcat gatgaaacag gttgaagacg atcggcgcgc
     1741 tgttgaccga aaaaaatcgt gccggctcgt cgcccgaacc attgaagtgc tgataggtac
     1801 aatttaacgg caatgcgaac aggctgccgt catgccattc gaattgggtc ttaacgccct
     1861 tgtcgttcca aatcgtagtt gctccgcgtc ccttgaggac aaaatgcaaa tcctcgtaaa
     1921 gatgtcgtac cgggttcaat tttttcccgg cggggatctc gctgacatac gcgccggtgg
     1981 cgccttccgc cccttccata ttgataaacg cgccgctgcc gccggtgcgc gcccagggtt
     2041 tgaggtcgac tttctttaag tcctggacga aaaaagtttt catgatggga atcccctcgc
     2101 ccaactgcca cttatcgtaa aaggggatat tatccatcac attctcggac tgtactgctg
     2161 gtttcgacat aaattccctc cgcaaaactt ggcaaaatta atatcgtatt gatgtcacga
     2221 aagcaaggtg gatcgagacc tgccacaacg cccgataagc gtgcaacgaa tctgtttata
     2281 gcattgactt tgaaggacaa aatctactaa atggcggcta cggtcggtgc tctctgacgc
     2341 tgatcgaaga ctgcatggcg aaaaaggaga gctcgcttgg caacatcgat tgaagccccg
     2401 atggtgggta aaattcttaa aatagaaaaa caagttggcg accacgtcga agaagacgat
     2461 gtcgtcgtcg tcatggaagc gatgaagatg gagattccgg tcgtcgctcc cgccgctggt
     2521 gtcgtcaccg aaatcaaagt ttctcctggc caagccgtcg aagccgagca gattctcgcg
     2581 gtcatcgaat aatcgcagat tgcattccgt tagctcggcg atctaatccc acaaggagat
     2641 ttcatggata cctggacgtt cggctggacg ctggcgttga tcgggatggg cgggaccatg
     2701 ctggttctct ggctcatcag cgtcatgatc ctgctcatta gaaaaatctt cccgtatcaa
     2761 gccgagccat cggcagcgaa gaaaggttag cacagtcaat ggaatctgcg ctcgtttccg
     2821 gcattcaggg tctatttgcc ggtgtcgtga gcatcacgat ttggcaaatc gtcatgctca
     2881 tcatcgcttc ggtgctgctc tacctcggaa ttaaaaaagg ctatgagccg ctgctactcg
     2941 tgcccatagg cttcggggct atcctcgtga atatcccgct cgccgacatg atgggcaaag
     3001 acggttttct ccgcttcttc tacgatgccg gcgtgctcac cgaagtgttt ccgttgctga
     3061 tttttatcgg aatcggctcc atgaccgatt ttcagccttt gctgcaaaat cccaaaatta
     3121 ttctgctcgg tgctgccggc cagttcggca tctttttaac cctgctgctc gctctggctc
     3181 tggggttcga caaactcgat gccgtcgcag ttgccattat cggcgcatgc gacggaccga
     3241 cggcgattta cgttagctca aagtttgccc cgcacatgct cggcgcagta tcggtcgccg
     3301 catattcgta catggctttg gtgcctttga tccagccgcc gatcatgcgc gccctcacca
     3361 ctgagaagga acgccagatc gtcatgggcg cggccaaatt ggaaccgatc tcgaagacca
     3421 ccaaaatcgt cttccccatc gccgtaacca ttttcgcctc gatcttggcg cctaaaggcg
     3481 cgccactcat cggtacgatc atgctcggca acctattgcg cgaaagcggt gtggttgagc
     3541 ggctcaagag cgcctcggag aacgagatcg ccaatatcgt taccttgctc ctcgggctgt
     3601 gcatcggcgc caccatggaa gcggataaat ttttgaaagc acaaacgctg ttgatcttgg
     3661 gactcgggtt tatcgccatc agcttggaca ccgttgtcgg actcctcttc ggcaagttga
     3721 tgtgcgcaat gaccggcggc aaagttaatc cgctgatcgg cgccgcgggc atttcagcat
     3781 ttccgatggc ggcccgcgtc gtccaagccg aaggccagaa atataataag aaaagttatc
     3841 tgttgatgca tgccatgagc gccaacgccg gcggccagat cggctcggtg atcgccgcgg
     3901 cggtgatgct ttcggtattg caaggaatgg gaatagtcgg cggatgatta ggcggtgcct
     3961 atggcgaaat cgcgcgaaga gctagagcaa aaactcgccg gtctggaaca aaaggtcacc
     4021 gcgggcgacc cgaaaaccgc ggctaagtta cacgagcagg gcaatcttac cgcccgcgag
     4081 cgcatcgaca aactgttaga cccgggcacc ttcgtcgaag aattcatgct cgcggaaacg
     4141 ccctgcatgg acttcggcat ggccgaacgc aagcaaccct ccgacggcgt cgtcaccggc
     4201 ttcggcaaga tcgaccaccg gccggtctat gtatactccc aagaccgctc gataatggcg
     4261 ggcagcgtcg gcgccgccca tgcggagaaa atcgcttacg tgattcagag cgcgcgcaaa
     4321 gttggcgtgc ccgtgatcgg cctcaacgac tccgccggcg cacgcattca ggaagggctc
     4381 agcgtcacct cagccatcgg caagattttc tttgagaaca gcatctcctc cggctgcatt
     4441 ccgcagatct ccgcgatcat gggtccctgc atcggcgtcg gctcctattc gccggcgctg
     4501 accgacgtca ttttacaggt acaaaactcc ggtcaaatgt tcatcaccgg ccctggcgtg
     4561 atcaaagaag tcttgggcga aaccgtcacc atggaagagt tgggcggcac caaaattcac
     4621 tcggaaattt ccggcgtcac cgacttggtg gcgaaaaacg acgaagaatg tttggcgatg
     4681 atccgccggc tggtcggttt cctaccggcg agtttcgagg aaaaaccacc gcgcaaaata
     4741 cccaccgacg atcccggccg catgctcgat agcttggaaa agctcgtgcc cgaggacatg
     4801 aagcgcgcct acaatatcct ccaggtgatc aaagccattg tcgacgacgg cgattttttc
     4861 gaattgaaag ccaaattcgc gcgtaatctc gtcatcggct tcggccggct cgacggctat
     4921 ccggtgggcc tagtggccaa ccagccgatg tttcttgccg gcagcctgga cgtcgacgcc
     4981 tcggacaagg cggcgcgctt tatccgcttc tgcgacgcct tcaatatccc ggtgattacc
     5041 ttgatggatg tgccgggttt cttccccggc aaacagcagg aggaaaaagg catcattcgc
     5101 cacggcgcca agatgctcta cgcctacgcc gaagccaccg tgccgaagat caccatcgtg
     5161 ctgcgaaaag gttacggcgg tgccaaacaa gcgctgtgca cgcgggagat gggcgccgat
     5221 cagttattcg tttggcccgg cgttgagctc gccgtcatgg gtgggggcgg cgcggtgaac
     5281 gtgctctata agaaagaaat cgaccagtcg gccgatccgg caaaaacccg cacagaaaaa
     5341 ctcgcggaat atctcgagcg cttcagcggc cccttcgaag cgctgtccaa acagtttgcc
     5401 caggctgcga tcaggcccgc cgatacccgc aagcggctga ttcaatcgct ggaaattttg
     5461 cgtaataaaa aggaagaacg gccgcgcaaa aagcacggcc tcatgccggt ttaagccgcc
     5521 ggctctggtt cgatctcggc cggcaaagag aaataaaagt tgctgccctt gccgggttcg
     5581 ctctcgaccc agatgcgccc gccatgggct tcgacgattc tcttacaaat catcaatccg
     5641 ataccggtgc tgcgactttg gctcttgtcg ctgctcgctc cctgcctata catttggaaa
     5701 atatcgccca gctcatcggc cgccatgccg acgccggtgt cgcgcaccga gacgacgatg
     5761 gcattttcgc tcgacctcgc ggacacctcg acggccccgc cgttgtccgt gaatttgagg
     5821 gcgttgctca aaatattcgt tagcacctgt ccaacccgcc ggggatcggc ccagagcacc
     5881 ggcaggcgct ccgcgatccg cgcgcttagt ctgatattgc gcttcttggc ttcgatggag
     5941 tgttccgtca cgatctcgtg aatttgcgat tcgatactta tccgttcgcg tttaagcact
     6001 agtttaccgg cgccgatgcg cgaaaaatcc agaaaatcgc tcacctgatc gatcagaccg
     6061 cggcagtttt tttcgatctt gcccaaccaa gtccgttgca acgcgttgac agcgccaaac
     6121 aacccctcct tcaccgactc cgccatcgaa acgatgttgc cgaggggcga gcggagatcg
     6181 tggatcacca tggcgttgaa gtcggccaat tgcttctcgg cattccggcg ctcttcgatt
     6241 tcgccggcca aattatcgat caggccgagt gaagcccggt agagctggcc gttctcgacg
     6301 ccgagggcga tctgccgagc catcgccttg agaaactgga tatcgccgac agcgaacgaa
     6361 cgtgaagaat tgtcgagcag aaccaacgta gtcctgcccc ggtccttacg cgttattggc
     6421 aagatcgccg cggctctgac cccatggtgt ggccataagg ccgcgagttc cggcacaaga
     6481 ctgacgtctt cgacaaacca ctcgccggta cattggctcg cagccggagc caactcatca
     6541 gccaaggatc ggtgcggcgc ctgcgcctca gcgccgcggc tcgccgagta agaatcggcc
     6601 gcgccgtcgg cgccgatgca ataaataatc gcatggtcgc agggcacccg cgctatcaac
     6661 gtctcaaggg tctgcggcat ggtttccgcc agggacaggg tttcagcgat ttcttgagcc
     6721 accgtctgaa gagtttccag ctgacgcgtg tgcatcgcca tctgttcttc gcctttgcgc
     6781 aaaagcgtaa tgtcgcgggt gatcgtcgcg acgccgcgga cgctcccctg gccatcgcgt
     6841 atcggcgaca gcgaagcgga cacgataatg cgccgcccat ctttacagat gcgttcgctt
     6901 tcgtaacgat tgatgccctt ccccaaacac accagctgca tcagttcagg gatctcgccg
     6961 cggtgctcgg ctggtattag cagcgaaata ttctggccgg tcatggccgc agcatcgtag
     7021 ccgtatattt gttcggcgcc gtcgttccaa ctcgagatca ccccgtcgag ggtcataccg
     7081 ataatgccat cttcagagga ttccacgatg gccgccaatt tggcccgggc ctcgtcggct
     7141 tcactgcgtt ttttcacata ttcgacaacc gatttactgc cgaccaacca ggccgcgctg
     7201 ctggcgctcg cggtcagcat ggcaatccag aacaagctcc acagcaaagt ctggttggct
     7261 tccccgtagg cgatcgcttt tggtacgctg gcgataacat tgatctggcc gccttcgcgg
     7321 ccgtcgccga cggttttaaa cgcataaagc cgggtgatcc cgtccagtcc ttggatttct
     7381 ttagtggttt gacggcgcag agggatcatt tccaagtgcg gcgcatcggg tagtcgctgg
     7441 ccaacccatg cttcatgatc gggaagccgg gtcagaattg tcccgtgact atcgagaata
     7501 gtgagtgtca tgcccgccgg cagattgctc tcggcggtta tctgctcgac ccgtttcagg
     7561 tccaaggccg cgaataggat ctcccggggc gcaccatccg tgccgagaat cggataaccg
     7621 aacgttagcg cccgccgccg ccagttgccg agcacctggt aatcgccgat gacaaagtct
     7681 ttggttgcca tgacgcgccg caccagagta ctccgcgcca agtcgaccga ggaaccgaag
     7741 tcgcttgcgc gacaccgcat cgcccccgct gggtcggaca cgccgatatc caagtaggct
     7801 cggtttcgcg ctaagaatcc gcgcacaatc tcttcacagg ggccccggtc aactctggca
     7861 atcgccggcg tctgagccag atgcatcaat aactgcttgg cggcatcgac caattgtgac
     7921 tggcgcaggg cgatgatgtt ggccaaccgc caggccgcct catggacttc gagaatttgc
     7981 acatcgcggt gaactttcgc ttgatacaag accaccgcaa gtattggcac cgcggcaatg
     8041 gcgatcatca gtaacagacg ccgccgcaac cggcgcaaaa atggctcggt tgccaactgt
     8101 acgatttttt ctatctggag cttagccaac ttcagtcccg ctctccgatg gctgcgctag
     8161 cggtctagtt cggtgccacc tagggcgctc taacggttag ccatggctcg cagggttaag
     8221 aatgggactt actttcgcgg ctttggaccc aactgatgag ctggctttcg cgcaggcgcc
     8281 attgaccgcc gactttgagc gccggcaaat cgccgctgcg aatcatccgt tgcaccgtct
     8341 tggtcgagac atggaggagc tcagccgctt ccgtgagggt gagtagctgc agggattcgt
     8401 tagttctttc cataggtttc gcgtttgcct acaaacgcaa tggcaatgcc aaacgacaaa
     8461 aacctaaacc attgtatttt atgcttttta gtgcgcacca atggaatgat ctaggatagc
     8521 ggaaacaaat ttgaacaaat cgatacatca gggtccatta ccggacgggt tggcgcagtg
     8581 taattcgcgc cgccgcgcag ctcgtggcgc gtcagtcgac tcactttgca cggagccaat
     8641 ctgcaatatt tcgaaaagtg attaaatcgg tagaggaagc attactggcg atagagtagc
     8701 gcaccagcat tggtggtatc gtagccgccc aaagcggcga ccgcgctctt gaagccgccg
     8761 gaacaaatcg tcgccagcag cagctcgccg cgcggcgatt cgaaaaatcg ccggtcaatg
     8821 agcaaatcgt attgttcgtt ggcgacggga atgaagtcca attttaacgc ctgcgccgcc
     8881 gcgcgcaccc ctaaaccggc atcggccaag ccgctcgcga ccgcgacgcc gaccgccata
     8941 tgggtgaact cttcgcgctc gtaacctgcg atcgctgcgc cgtcgagctg tagtttcttg
     9001 agctggtaat ctaacaaaac tcgcgtgccc gagccctgct ggcgattgac gaagcgcagg
     9061 tcgcgacgcg ccaggtccgg caatccgcgc agcgctttcg gattgccgcg gctgacgatg
     9121 atgccttgct cgcgctgtgc cagatgaatc aaggcgactg caacattggg aattgcccgc
     9181 tgaatatccg gaatgttgta gacgccgctc gcgggatcga gcagatgagt gccggcgata
     9241 tgagtttcgc cgcgctgcag cgccaagagg ccgcccaagc taccgacgtt ggtcgcggcg
     9301 attttcaact ccgggcggcg caatttcaat tgatcttcca acacgccgat cgccaggtca
     9361 tgactgccgg tgcagagaat cgtgttctcg acatcgacca tcggccgcaa cagttcgact
     9421 tcggcatcct cgccggcgtt gagcccttca accaaactgg ggatgcgcat gagtccgtcg
     9481 gcatgcacca tcgtggtgat cacgccggcg ccgcgcgcca aaggcacggc gaccagtttg
     9541 tcgccgaccc gtcccaaggt cacacgaata aactcttcca gacccaagtg tgaggcgatc
     9601 ttcttcggca cgatggcgcg caccgacggc aacgtcgtcg cggcgttgct gagaaatttt
     9661 tccaacgccg ggcggagaat ttcccgggca atgacgattg ccgacaccgg atagcccggc
     9721 acgccaagca ccggcttgcc ggctaacgac cccagcacag ccggtttacc tggcatcacg
     9781 tcgatgccat gggcgagcag ttcgccggcg gcggcaacca catcggcggt gagatcatgc
     9841 tccccagccg atgagcccgc gataatcgtc acgagatcgc actcggcgcc ggcgcgctcc
     9901 agtgccgccc gcagcaatgt ctcatcgtcc cgggcacgcg ggtatttgac cggctcaccg
     9961 ccccattcgc ggacgaaggc cgccagcacc gttgaattaa aatcgattac cgcccccggt
    10021 gtggcttctt ctcccggctg aatcagttca tcgccggtgg gaatgatgcc gacccgaggc
    10081 cgcgccttga cccgcaccgt ggtatgaccg gccgccaaca acgcgcccag atcgtagggg
    10141 cgtagccgat gggaacgggg caacagcaac tcggtggcga caacatcttc gccgaccaag
    10201 cgcacatgat tccacggcgc caccgattcg aaaatctcga tggtggtcgc atcgacctgg
    10261 tgcactttct cgatcatgat gacggcgttg gcccaggccg gcagcgcgtt gccggtatcg
    10321 acgtagcgaa aaccatcggc gcggggagaa tccgcatcga tcaatgtgag tttcttgccg
    10381 acgaactctg tcgcaccgaa ggtctcttcc gcgcgcacgc agatcccatc catcgccgag
    10441 gcatgataat gcggcgagga aatcttggcg aacactggct cggcggtaat ccggtcgagg
    10501 ccgtcgtcga tggcaatctc ttcggtcgct aacgccgccg gattgaccgt cgacaaaaat
    10561 agctcgcgcg cttcgctgag cggtgttttc tttaaatatc tcttacgcgc cataagccaa
    10621 tctgtttgtt cggagcgtca ttcaccgtgc tgaccgctga tcgctagaac aaaatcacat
    10681 cgacgtcttc accctgttcg aggccgtcct gattcatgcc gatacgcacc atgccgtcgg
    10741 ccttgaccag agtgaaaatc gcgccggatt tgctcggtaa tggcgccgcg acaaagctcc
    10801 caccctcacc ttccatgacg acgcggacgt agtcctctcg gccgatctgc gaagcgatgt
    10861 tggtctttaa ctttgcgcgc acggtgtgac tgaaacgctg caaagcgtca cggctctcgc
    10921 cgcccaagcg gcgcagcatc ggcgcgccga acaaatcgaa gattaccaga gcggaaaccg
    10981 gatagcccgg caatccgagg atcggcttgc cgcatgcttt ggcgaagatc gtcggcttgc
    11041 cgggcgcgat ggagatgccg tggaaaataa attgcgaatc gggaaacgac aggattgttt
    11101 ccaaggcgat gtccttggcg cccatggagc tgccgccgga gagcagcacg gcgtccgccc
    11161 agtcgagcgc cgcaccgatc gctgcggtca gcagcgcgcg atcgtcgcgg atcacgcccc
    11221 aatctttgag ctcgccgccg gcttcttcga tcaagccggc gagcgaatgc tgattgatgt
    11281 tacgcacctg gcccggctgc gggtcgaccg cggcatcgac gatctcgtcg ccggtggaga
    11341 tcagcgcgac ccgcggccgt ttgtagaccg taatcgattc gatgccgacg ccggtaagcg
    11401 cgcccaaatc atggccgcgc agccgccgac cttgagcaaa aaccggctcg cctcttttga
    11461 tatcgtcgcc gatttgaatg acgttttgcc cgggcgacac ggcgcggtga atttccacca
    11521 agccgccgtc ggtctcgtcg gtatactcga ccatcacgac ggcgtcggca tccggcggca
    11581 tcatgccgcc ggtggaaatg cgcatcgctt cgcctttgtc gagagcctga gtcgcttctt
    11641 tgcccatctc gacgacgccg gccaatttta gataagccgg caaactttgg ctcgcgccaa
    11701 aagtgtcctt ggctttgacc gcgtaaccgt ccatcgccgc gcgatgaaaa tgcggcagat
    11761 cgacctggga gaagagatct tcggccagaa ctcgacttct ggcgcgaaca ctcgccactg
    11821 tttccgttcc caccggaagc acttcgagaa gaatgcgtct cgcttcttgc ggcggttcaa
    11881 ccttaaaaaa atctttcaga tagccccctc atggcggctt atatagaaac acttttgtga
    11941 acgcaaggta aagtgccgcg caggcgcctc tccgtgctcg ctgccggatg catcgagcag
    12001 cctacgcgcc cgctgacttt tgaatgagca gctgcgcttg ccgcgcaggg gcgatcgaag
    12061 ccgcggtggc aaagtttttc ttcgggcgat acacgcgcgc ttttgctcgt gggccagtcg
    12121 ccgatcacgc acaaattact tggtaagcag tgactacagg acgcaaggat ggagcagcgc
    12181 gcgccacgtt catgccgcag attgtcattg cgtctttcaa tctcgccacg cctgagataa
    12241 atgtggcgct aatgaacgat acgccggcag gagaagcgac ggcggtcatt ctggccggcg
    12301 gcaaaagctc acgcatgggt cagcccaaag cgctcctgct ctttgccggc gagccgttga
    12361 tcgttcacct ggttcgtcga ctcggtcaat tgttcagcga cattgtagtg gtcgccgcac
    12421 ccgaacagga tctgccggtg ctgtcagcga cgatcgtgcg cgacgatgtc gcgcatcagg
    12481 gccccgtcgg cggcatctat tatggacttg aggccgcgcg ccgagaaatt tgttttgtcg
    12541 cttcgtgcga tgccccgttt ttgaatctgc gtttaatcgc ttatctcatg acgcaaatat
    12601 cagatttcga tgtggtggtg cccttcatcc aggaccgtta tcagccgctc cacgcggtct
    12661 atcgcaaaca ggtggcgcca cttttgaaag aacaactaga gcgcggtgag ctgcggccga
    12721 tttttctcta tgacaaagtc cgcacccgaa aggttaccga agct
//
LOCUS       DASQKS010000186         7897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1357122, whole genome shotgun sequence.
ACCESSION   DASQKS010000186 DASQKS010000000
VERSION     DASQKS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357122"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VE170_04830"
     CDS             <1..303
                     /locus_tag="VE170_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HYJ14790.1"
                     /translation="QPELFVEISPELAQEKGIKNLDWCKISSPRTQIRAKALVTRRMR
                     PFKIDGKVIHQVGMPWHWGYEGLSTGDVVNELTSLIADPNVSMHEGKAFVCNVEKA"
     gene            406..1209
                     /locus_tag="VE170_04835"
     CDS             406..1209
                     /locus_tag="VE170_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYJ14791.1"
                     /translation="MAEPMGFYTDTTVCIGCKACEVACKEWNQLPSTNGGVNNTLSGD
                     SYDNTRALDGIHWRHVKFIEQFTEDRKDGRWLLMSDVCKHCVNAPCLEVCPTGAIVRT
                     EFDTVVIQSDACNGCRDCISACPFGVIEVNPASGTAQKCTLCYDRMQGGLEPACSKAC
                     PTQSIQFGTINELRGRAQKRVDQLQQMGEKRAYLYGADEKILGGLNSFYLLVDKPEVY
                     GLPPDPKMATRNLKSGTWFSALGAVLVGLMGIINFRNRGARINAREDQP"
     gene            1206..2111
                     /gene="nrfD"
                     /locus_tag="VE170_04840"
     CDS             1206..2111
                     /gene="nrfD"
                     /locus_tag="VE170_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HYJ14792.1"
                     /translation="MNQFPSSTFFSAPPDWGWYIVFYFFFGGLAGGCYFLAVLIDLFS
                     RPEDRPLARLGYYVSFPCIVISGLLLTLDLTRPERFWHMLIQSNTYLPIFKPWSPMSV
                     GSWALTIFGIFSLLGFLSALAEAGHWQRPAARRLRAPGLLGSVVAVVGGLFGFYVAGY
                     TGVLLAVTNRPIWSDTPLLGMLFVVSAASISAALMILLAQRSGRALPGVNALHRFDTW
                     VIALELVVLVAVMISLGPVFRAWLNAWGILLFFGVIVVGMLLPLALYWRRQWFGALNV
                     TASAAAVLAGGFILRLVIVFSAQGV"
     gene            2113..2343
                     /locus_tag="VE170_04845"
     CDS             2113..2343
                     /locus_tag="VE170_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14793.1"
                     /translation="MAHITIFFLVVLCALAACASPETGRARGAGPGADIGNRTKVVRM
                     HEGADPFYKTPKLIPGQPPPLAPARQADTLSR"
     gene            2343..3224
                     /locus_tag="VE170_04850"
     CDS             2343..3224
                     /locus_tag="VE170_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HYJ14794.1"
                     /translation="MTASIDSDVQDLGRQRPEWQPWLSVVQQVLRETADVKWDTVIPD
                     RAEPLENKAPVLAGASIAIETSWLRRWTQHLIGTASRSGTEKMATLKGVLQGTTHTLS
                     LFKAALRLDQSALKQIAVNCDADPEAFQAVTALLPVPFLHACNRRWANSLPQGWAEGY
                     CPVCGSWPAVAEVRGIDRSRYFRCGRCASAWPVNCLVCAYCGMTDHEQLVSLVPEQGG
                     NSRVIEACKHCLGYVKTFTTLQASPAARVILDDLASVDLDIAAVEHGYKRPQGAGYLF
                     DVTVVDKITDKFSFWRA"
     gene            3225..3611
                     /locus_tag="VE170_04855"
     CDS             3225..3611
                     /locus_tag="VE170_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14795.1"
                     /translation="MDPPIRPAQVCQALLAALEAAEGQRKRRKRDQTPDAIGLAVKRE
                     LLERAVDADPEPEVFEAWLLNYSQTSVAAALAGPASAMARSVFDEWRLAHSLKEFNVW
                     LNQGAPSADVADGPQESPHTNVRETR"
     gene            3898..4848
                     /locus_tag="VE170_04860"
     CDS             3898..4848
                     /locus_tag="VE170_04860"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ14796.1"
                     /translation="MDKPIKLSIAATSKRVDIANVVGYTFFNSIELPRQSQLDIAMGW
                     RDSELGGTGPALLVGKKQYSFGFGNPVGLSAMAYLGCGFYKKKIPLRAIGVFPTWDRL
                     IFAVRPDTGIQSLEDIRKQKYPLRISTRRRGKLATTIYAIEEVLKAYGMSYKDIEKWG
                     GKIMEAPNPSGSERRDAILSGAADAVFDEGVKSWGPTALKAGMRFLPINDNAVKHMAK
                     LGFPSAMLTRKHYPRLAQAIRCVDFSGWTFFCHADLSDKVAYHMARAVDLCHKHIPVD
                     HFDKRPMTIREFCRGGEAGQLNIPLHPGAKKYFREKGYID"
     gene            4841..5836
                     /locus_tag="VE170_04865"
     CDS             4841..5836
                     /locus_tag="VE170_04865"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14797.1"
                     /translation="MIKIFGFMQTVALAALLTIASLGQPIAAHGEQFRVALSTKDFGY
                     LPLFVGMRAGLFAQENLEIQWIVVNSNVVVTALLAGEIDVAGIAGSSMRAAARGAPLK
                     ANFFPYDKSLFVLMGAPNIKRVQDLKGKVIGTTGPGATTEVAASMVLQHHGMDPKRDV
                     TFMTIGGAETSIVAMRNGIIQARAFNPDAAYIMKKQGFTELGVLADMGPWPWAGYSTS
                     DAHLANRRDKLKRWTRAMVKSLQLMVNRKEETYRFAQAHFAHARDVIEAAANVCIKAI
                     DAQNPGGATDDAMRKNIDLTITQPLKLAASPPLAQLVDFSLLREVHKELGIAGRK"
     gene            5833..6897
                     /locus_tag="VE170_04870"
     CDS             5833..6897
                     /locus_tag="VE170_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14798.1"
                     /translation="MNWRTFPIPVVVALLLISRESHAQSLDAKLAVLEKLPAAERQQK
                     LLEGAKAEGEAVIYANMDVSAMKPLTDGFMKRHPGVKATSVHISGAGIITRVEAEARA
                     GRSLSDVVLSGQLGVLALLERKIFGRYRSPEREQYRDGYKDKDGLWTAYMNNVMVSVY
                     NTRQVKKDEAPRGLEDLLKPRWKNKLAMDSQSYVWFGTILQYLGEEAGLRFMKRLNEQ
                     NLSHQRGRRLISQLVAAGEFDMAVETNLNSVLSLSRQGAPLWFAPAPPFFLTPSLVFV
                     NASAPHPFAGALFTDYLLSEEGQKIIVSTNRMPAHVKVKSPESQLIEGQDVRMPDIFD
                     IGRRYQAIGNRYRETFPGAR"
     gene            7024..>7897
                     /locus_tag="VE170_04875"
     CDS             7024..>7897
                     /locus_tag="VE170_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14799.1"
                     /translation="MAKTKITLGLPDHPRCQAIIDGKVGIEGYELDVDPNFVSSGERH
                     YKYLHGEWDVGEVSAASLMRAIEKGQELLAIPVFFERGPRQRNIYHCEQHLKHPSELK
                     GKKFGCFRYGATAVVWARGYLLDAHGIKTTDMQWFVSGREVFIGHELPVKVQRIDPPP
                     PFGGEKLVLSKMLNAGELHGAVVPGDNGYAGFFGGGETPPMMAAYAGVKPLFDNNQEI
                     LDYVKKTGINPIMHVLSIRKEVVARHPDFPAKLTRAFMEARDVSTKYMPAEEIAGYQR
                     ERDALGEDPYDYTMR"
BASE COUNT         1685 a         2383 c         2217 g         1612 t
ORIGIN      
        1 cagcccgagc ttttcgtcga gatcagcccc gagctggccc aggagaaagg cattaagaac
       61 ctcgactggt gcaagatctc cagcccgcgg acccagatcc gcgccaaggc tctggtcacg
      121 cgccgcatgc gccccttcaa gatcgacggc aaagtcatcc accaggtggg catgccctgg
      181 cactggggct atgaaggcct ttcgacggga gacgtcgtca acgagctcac gtcactgata
      241 gccgatccga acgtttcgat gcacgagggc aaggcttttg tgtgtaatgt ggagaaggca
      301 tagcgcctat ggcgcattcc agattacaaa tttcaaattg ccactgccga gaatctgaat
      361 ctggaatttg taatttgtca ttcgcgaagc gaagaggagc gatttatggc agaacccatg
      421 ggtttctaca ccgacactac agtatgcatc ggctgcaagg cgtgcgaagt cgcctgtaag
      481 gaatggaatc agctccccag caccaacggc ggcgtcaaca acactttgag cggcgatagc
      541 tacgacaaca cccgcgccct cgacggcatc cactggcgtc atgtgaaatt catcgagcag
      601 ttcactgaag accgtaagga tggccgctgg ctgctgatga gcgacgtgtg caagcactgc
      661 gtcaacgctc cctgcctcga ggtctgcccg accggcgcga tcgtgcgcac cgagttcgat
      721 accgtggtca tccaatcgga cgcctgcaac ggctgccgcg actgcatctc ggcctgtcct
      781 ttcggcgtca tcgaagtcaa tccagcttcg ggcaccgcgc agaagtgcac gctttgttat
      841 gaccggatgc agggcggctt ggagccggcc tgctcgaagg cctgtccgac acagtcgatc
      901 cagtttggca ccataaatga gttgagggga cgggcgcaga aacgcgtgga ccagctccag
      961 cagatgggcg agaagcgagc gtatctttat ggcgcggatg aaaaaatcct gggcgggctg
     1021 aattcgtttt acctgctggt ggataaaccg gaagtctacg gcctgcctcc cgatccgaaa
     1081 atggccaccc gcaatctcaa atccggcacc tggttctccg ccctcggcgc cgtactggtc
     1141 ggtttgatgg gcatcatcaa tttccgcaac cgcggcgccc gcatcaacgc tcgggaggat
     1201 caaccatgaa ccaattcccc tccagcactt ttttcagcgc gccgccggac tggggttggt
     1261 acattgtctt ttactttttt ttcggcggcc tggccggcgg atgttatttt ctcgccgtcc
     1321 tgattgattt gttcagcaga ccggaagacc gtccgctggc acggctggga tactacgtct
     1381 cgtttccttg catcgttatc agcgggctgc tgctgacact cgaccttacc cgacccgaac
     1441 ggttttggca tatgctgatc cagtccaaca cctatctacc catcttcaag ccctggtccc
     1501 cgatgtcggt gggatcatgg gcgctgacga tcttcggaat tttttcgttg ctcggttttt
     1561 taagcgcgct tgcagaagct ggccattggc aacggcccgc ggcccgcagg ctgagggctc
     1621 ccggcctgct cggctcggtg gttgctgtgg tcggcggact cttcggattc tatgtcgccg
     1681 gttatacggg agttcttcta gccgtcacga accgaccgat ctggtccgac acgccgctac
     1741 tcggcatgct tttcgtggtt tcggcggcgt cgatatcggc ggcattgatg atactgctgg
     1801 cgcagaggtc cggccgcgcg ctgcccggcg ttaatgcgct gcatcgcttt gacacctggg
     1861 ttatcgcgtt ggagctggtc gttctcgtcg ccgtcatgat ctccctgggc ccggtatttc
     1921 gcgcctggct gaacgcctgg ggtatcctgc tgttttttgg cgtcatcgtc gttggcatgc
     1981 tgcttccctt ggctctttac tggcgcaggc aatggttcgg cgctttgaac gtcaccgcat
     2041 ccgctgcggc ggtcctggcc ggcggtttta tactgcgcct ggtgatcgtt ttttcggctc
     2101 aaggggtttg acgtggcgca cattacgata ttctttttag tcgttctctg cgctcttgcc
     2161 gcctgcgcga gtccggaaac cggccgggcg cgcggcgccg gtcccggcgc cgacatcggc
     2221 aaccggacca aggtcgtgcg catgcatgaa ggcgcagacc ctttctacaa aaccccaaaa
     2281 ctcattcccg gccaaccgcc accgttggcg ccggcgcgcc aagccgacac gcttagccgc
     2341 tgatgacggc ttccatcgat agcgatgtcc aggatctcgg ccgccaacgg cccgagtggc
     2401 agccctggct ttctgtggtt cagcaggtcc tcagagaaac cgctgacgtt aaatgggaca
     2461 cagttatccc ggatcgcgca gaaccgttgg aaaacaaggc gccggttctg gcgggcgcct
     2521 cgatcgcaat agaaacgagt tggctgcgcc gttggaccca gcacctgatc ggcaccgcca
     2581 gccgtagtgg cacagaaaaa atggccacgc tcaagggcgt gctgcaaggg acgacccata
     2641 ctctttcttt gttcaaagct gccctccgtc tggatcaaag cgcgctaaaa cagatcgcgg
     2701 tgaactgtga tgccgacccg gaagcatttc aggcggttac agcgctgctg cctgtgccct
     2761 tcctccacgc ctgcaatcgc cgctgggcca attctctgcc tcaaggttgg gctgaaggct
     2821 attgccccgt atgcggtagc tggcctgccg tcgccgaagt gcgcggcatc gaccgcagcc
     2881 gttattttcg ctgcggccgc tgcgccagtg cgtggccagt caactgtttg gtgtgcgcct
     2941 attgcggcat gaccgaccat gaacaattgg tctcgctggt accggagcaa ggcggcaata
     3001 gtcgtgtgat tgaagcctgc aagcattgtc tcggctacgt caaaaccttc accaccctcc
     3061 aggccagtcc cgccgccagg gtaatactcg acgacctcgc cagcgtggat ctcgatatcg
     3121 cggccgtgga gcatggttac aagcgtcctc aaggagcggg ttaccttttc gacgtgacgg
     3181 tggtcgataa aatcactgac aaattttctt tttggagagc ataggtggat ccgccaattc
     3241 gcccagccca ggtttgccag gcgttgctcg cggcgctcga agcggccgag ggacaacgaa
     3301 aaagacgcaa gcgtgaccaa actcccgacg ccatcgggct ggcggtgaaa cgggaactgc
     3361 tggagcgtgc cgtcgatgcc gaccctgagc cggaagtctt tgaagcatgg ctgctcaatt
     3421 attctcagac cagcgtcgcc gctgcattag ctggcccggc gtccgccatg gctcggtccg
     3481 tgttcgacga atggcggttg gctcattcgt tgaaagaatt taatgtttgg ttgaatcagg
     3541 gcgcgccgtc ggctgatgtt gccgacgggc cccaagaaag ccctcacacg aatgttagag
     3601 aaactcggta gaacgcccgc cgactcgcgc cgcaggtgtt gcggcttgca gaccaccaat
     3661 caagattcgt aaccacgtcc aacctgccgt ttcttaacat tccacaatca gtccacacgc
     3721 gaatccacga cagatacaat ttaattttct acctgtaccg agccggtatc aggtgatgaa
     3781 gtttgcagat cactctgttt gttatataaa cccaaattgg cgagaccgtt ccgacggccg
     3841 gcgcgcgcgg caagcggttt aaatcccggt caacaacact acataaggcc cacaacaatg
     3901 gacaaaccca taaaactctc catcgctgca acctccaaac gcgtcgatat cgccaacgtc
     3961 gtcggctata cctttttcaa cagcatcgag ctgccgcgcc aatcgcaact cgacatcgcc
     4021 atgggctggc gcgattccga gctgggcggc accgggccgg cgctcctggt cggcaagaaa
     4081 caatactctt tcggatttgg caatccagtc ggcctgtcgg cgatggctta tctcggctgc
     4141 ggtttctaca aaaagaaaat cccactgcgc gccatcggcg tctttccgac ctgggaccgc
     4201 ttgatcttcg ccgtgcgccc ggacaccggc atccagtcgc tggaagacat aaggaagcag
     4261 aaatatccgt tgcgcatctc gacgcgccgg cgcggcaagc tggctaccac gatttacgcc
     4321 atcgaagagg tgctcaaagc ctacggcatg agctacaaag acatcgagaa gtggggcggc
     4381 aagatcatgg aagcgcccaa tccgagcggt agcgagcggc gcgacgcgat tttatccggc
     4441 gcagcggatg ctgtcttcga cgaaggggtg aaaagctggg ggccgacggc attgaaagcc
     4501 ggcatgcgct ttttgccgat caatgataac gcggtaaaac acatggcgaa gctcggcttc
     4561 cccagcgcca tgctgacgcg caagcattat cccaggttgg ctcaagccat tcgctgcgtc
     4621 gatttcagtg gctggacatt tttttgccac gccgacttat cggacaaggt cgcctaccac
     4681 atggccagag ccgtcgacct gtgccacaaa catatccccg tcgaccattt cgacaaacgg
     4741 cccatgacca ttcgcgagtt ctgccgcggc ggcgaagctg gacaactaaa catcccgctg
     4801 catcccggcg ccaagaaata ttttcgcgag aaaggctata ttgattaaga tcttcggctt
     4861 catgcaaacc gtcgcattgg cggcgcttct gacgattgca tcgcttggcc aaccaatcgc
     4921 ggcccatggc gaacagttcc gcgtcgcgct ttcgaccaaa gacttcggct atctgccgct
     4981 gttcgtcggc atgcgcgccg gtctattcgc ccaggagaat ctggaaatcc agtggatcgt
     5041 ggtcaatagc aacgtggtcg tcaccgccct tctagccggc gagatcgatg tcgccggcat
     5101 cgccggttcg tcgatgcgcg cggcagcacg cggcgcgccg ttgaaggcta actttttccc
     5161 ctatgacaaa tcgctgttcg tcttgatggg cgcgcccaat atcaagcgcg tgcaagattt
     5221 gaaaggcaaa gtgatcggca ccaccggtcc cggcgccacc accgaggtcg ccgcttccat
     5281 ggtgctgcag catcacggca tggatccgaa aagagatgtc accttcatga ccatcggcgg
     5341 cgccgagaca tccatcgtag ccatgcgcaa cggcatcatc caagcccgcg ccttcaatcc
     5401 cgacgcggca tatatcatga aaaaacaagg cttcaccgag ctcggcgtgc tcgccgacat
     5461 ggggccctgg ccttgggccg gttattcaac ttccgacgcg catctggcga accggcgcga
     5521 caagctcaaa cgctggaccc gcgccatggt gaaaagcctg cagctgatgg tcaatcgcaa
     5581 agaagagacg tataggtttg cccaggccca cttcgcccat gcgcgcgacg tcatcgaagc
     5641 tgcggcgaac gtctgcatca aagccatcga tgcgcaaaat cccggcggcg ccaccgacga
     5701 cgccatgcgc aaaaatatcg acttaaccat cacccagccg ttaaagctcg cggctagccc
     5761 gccattggcg cagctggtcg acttttcctt gctgcgggaa gtccacaaag agctcggtat
     5821 agcggggcga aaatgaactg gcgaactttt ccgatacccg tggttgtcgc actattactg
     5881 atatcacggg aatcacatgc gcagagcctc gatgccaagc tcgcggtgtt ggagaaacta
     5941 ccggcggcgg aacgccagca aaaattgctg gaaggggcca aggccgaagg tgaagcggtt
     6001 atttacgcca acatggatgt cagcgccatg aagccgctca ccgacggctt catgaaacgc
     6061 catcccggtg tcaaagccac tagcgtgcat atttccggcg cggggatcat aacccgggtc
     6121 gaagcggagg cccgcgcggg acgctcgctc tccgatgtcg tcctcagcgg ccaactcggc
     6181 gtgctcgcct tactcgaaag aaaaatcttc ggccgctacc gctcgccgga gcgcgaacaa
     6241 taccgcgacg gctacaagga caaggacggc ctgtggacgg cctacatgaa caacgtcatg
     6301 gtcagcgtct acaacacccg tcaggtaaaa aaagacgaag cgccgcgcgg cctcgaagat
     6361 ctactgaaac cccggtggaa gaacaagctg gcgatggaca gccagtccta cgtctggttc
     6421 ggcacaattt tacaatattt gggtgaagaa gccggcttgc gtttcatgaa gcggttaaac
     6481 gagcaaaacc tgtcgcacca gcgcggccgc cggctgatca gccaactggt cgccgccggt
     6541 gagttcgaca tggcagtcga aaccaatttg aactcggttc tgagtctgag ccgtcaaggc
     6601 gcgccgctct ggttcgcccc ggcaccgccc tttttcctga cgccgagcct ggtgttcgtc
     6661 aacgccagcg cgccccatcc cttcgccggc gctttattca ccgactattt gctctcagaa
     6721 gaaggccaaa aaattatcgt cagcaccaat cgcatgccgg cgcacgtcaa agttaaatcg
     6781 ccggagagcc agcttatcga aggccaggac gtgcgcatgc cggatatttt cgatatagga
     6841 agacgctacc aagcgatcgg caaccggtat agagaaacgt tccccggcgc tcgttagcgc
     6901 gaagcgcgaa gcgcgcgatt acaaactcca gattacaaat tccagatacc ggagtttgca
     6961 ttcaatctgt aatttggaat ttgtaatctg gaattttcaa cctgaaactg tgaaaggacg
     7021 ttaatggcta aaaccaaaat caccctcggc ctccccgacc acccgcgctg ccaggcgatc
     7081 atcgacggca aagtcggcat cgaaggctac gaactcgacg tcgatcccaa ctttgtctcc
     7141 tccggcgagc gccattacaa atatctccac ggcgaatggg atgtcggcga agtctcggcg
     7201 gcgagcttga tgcgcgccat tgaaaagggg caagagctgc tggcgatacc ggtgttcttc
     7261 gagcgcggcc cgcgccagcg caatatttat cactgcgagc aacacctaaa gcatccgtcc
     7321 gagctcaaag gtaaaaagtt cggctgcttt cgctacggcg ccaccgcggt ggtctgggcg
     7381 cgcggttact tgctcgacgc ccatggcatc aagaccaccg acatgcagtg gttcgtctcc
     7441 ggccgcgaag tgttcatcgg ccatgagctg ccggtcaaag tccagcgcat cgacccgccg
     7501 ccgcccttcg gtggcgagaa gctggtgctg tcgaaaatgt taaatgcagg cgaactgcat
     7561 ggcgccgtcg taccgggcga caatggttat gccggcttct tcggcggcgg cgaaaccccg
     7621 ccgatgatgg ctgcgtatgc cggcgtcaag ccgctattcg ataataacca agagatactc
     7681 gattacgtga agaaaaccgg catcaacccg atcatgcatg tcctgtcgat ccgtaaagaa
     7741 gtcgtcgccc gccatcccga ttttccggca aagctcacgc gtgctttcat ggaagcccgc
     7801 gatgtgtcta cgaagtacat gcccgccgaa gaaatcgccg gctaccaaag agagcgcgac
     7861 gccctaggcg aagatcccta cgactacact atgcgtg
//
LOCUS       DASQKS010000187         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_98735, whole genome shotgun sequence.
ACCESSION   DASQKS010000187 DASQKS010000000
VERSION     DASQKS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98735"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VE170_04880"
     CDS             <1..906
                     /locus_tag="VE170_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ14800.1"
                     /translation="FLMYAPFKGLSRTYTAVHQGLAGAERVFEILDEKPGITDRPGAQ
                     PAKPFTSKIELQNVSFAYGKSPVLKNIDLIIEAGQMVALVGMSGVGKSTLVDLIPRFY
                     DVNAGRILVDGVDIRELTVQSLRGQIGIVTQHTFLFNDSIRNNIAYGDPERDMEAIIA
                     AAKAAHAHEFIIAMPNGYDSEIGEMGLQLSGGQRQRIAIARALLKDAPILILDEATSS
                     LDADSERSVQEALETLMRTRTTIVIAHRLSTIRKADRIVVMVDGAIAEEGTHDELLAR
                     DREYSRLYSLQLLENRSVNEVELVH"
     gene            920..>2053
                     /locus_tag="VE170_04885"
     CDS             920..>2053
                     /locus_tag="VE170_04885"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HYJ14801.1"
                     /translation="MWFFGYNILLVMVFLLTLPFMPLLQLLGKRFSAGLAQRLGYFPP
                     AQVHNMAGARPVWIHASSVGEVGSVIALVRELKNRHPQRRIMLSTFTATGNENAQKCA
                     GVDAVIYFPLDFLWTVRRALTQWNPSLMLFVETEIWPNMLGECFRRGIPAVLLSGRLS
                     ARSFPRYRRFRGFFRHVLRRFAAIGMQTGEDAARIVRLGADANKVSVVGSLKWAGAPP
                     PASQFADVPRHGKKLLIAGSTHAGEEQILLACFAILRPRFPELSMVLAPRHPERFGEV
                     EALLMSSGFSWCRRSMAEPDRLFAKDILLLDTVGELSQFFALGDIAFVGGSLVDVGGH
                     NILEPARAGKAILFGPYMANMQAVAEAFKAQGAAVEVADGVALS"
BASE COUNT          390 a          579 c          627 g          457 t
ORIGIN      
        1 tttttgatgt atgctccttt taaagggttg agccgtactt acacggcggt ccatcagggg
       61 cttgcaggcg ccgaacgggt cttcgaaata ctcgacgaga aaccggggat taccgaccgg
      121 ccgggcgccc aacccgcaaa accgttcacg agcaaaatcg agctgcaaaa tgtcagcttc
      181 gcctatggca agagtccggt acttaaaaat atcgatctca tcatcgaggc cggccaaatg
      241 gtcgccttgg tgggcatgag cggcgtcggt aaaagcaccc tggtcgacct gatcccgcgt
      301 ttctacgacg tcaatgcggg ccgcattcta gtcgacggtg tggatattcg cgagctcacg
      361 gtgcaatcgc tgcgcggcca aatcggcatc gtcacccagc atacttttct gttcaacgac
      421 agcattcgta acaacatcgc ctacggcgat ccggagcgcg atatggaagc gatcatcgcc
      481 gccgccaagg cggcccatgc gcacgagttc atcatcgcga tgcccaacgg gtatgattcg
      541 gagatcggcg aaatgggctt gcaactctcc ggcgggcagc gccaacgcat agcgatcgcg
      601 cgggctttat tgaaggatgc gccgatcctg attctcgacg aagcgacttc gtccttggac
      661 gccgattcag aacgctcggt gcaggaagca ttggagactc tgatgcgcac gcggacgacg
      721 atcgtcatcg cccatcggtt gtcgacgatc cgcaaggccg accggatcgt cgtcatggtc
      781 gacggcgcca tcgctgagga aggcacccac gacgagttgt tggcgcggga tcgagagtac
      841 agccggctgt actcgctgca actgctcgaa aatcgttcgg tcaacgaagt cgaattggtg
      901 cactaggacg ccatctgcca tgtggttctt tggctataat atcctccttg tcatggtgtt
      961 cttgttgacc ttgccgttca tgcctttgct gcaattgctc ggcaaacgat tcagcgcggg
     1021 gctggcccag cgtctgggtt atttcccgcc agcgcaggtg cataatatgg cgggggccag
     1081 gccggtgtgg attcatgcct cgtcggtggg ggaagtgggt tcggtcatcg ccctggtgcg
     1141 cgagttgaaa aatcgccatc cccagcgccg cataatgctg tcgacgttta ccgccactgg
     1201 caatgaaaac gcgcagaagt gtgccggggt cgatgccgtg atttatttcc ccttggattt
     1261 tttgtggact gtcaggcgtg ccttgaccca gtggaacccg tcattgatgc tgttcgtcga
     1321 gaccgagatc tggcccaata tgttgggcga atgctttcgc cgcggcatcc cggcggtgct
     1381 gctgagcggg cgtttgtcgg cacgctcctt tccgcgctac cgaagattcc gcggtttttt
     1441 tcgccacgtg ctgcggcggt tcgccgccat cggcatgcag accggcgaag atgcggcgcg
     1501 gatcgttaga cttggcgccg atgccaacaa agtgtcggtg gtcggtagtc tcaaatgggc
     1561 cggagcgccg ccgccggcgt cgcaattcgc cgatgtgccg cgacacggta aaaaactgct
     1621 gatcgcaggc agcacccatg ccggtgagga acagatactg ctggcctgtt tcgctatcct
     1681 gcggccccgc tttcccgagt tgtcgatggt cttggcgccg cgtcatcccg aacggtttgg
     1741 cgaagttgag gcgctcctaa tgagtagcgg ctttagctgg tgtcgcagga gtatggccga
     1801 acctgatcgg ttatttgcca aggacatttt gctgctcgat acagtcggtg agctcagtca
     1861 attttttgct ctgggcgata tcgcgtttgt cggcggcagt ctggtcgatg tcggcggcca
     1921 taatatcctc gaaccggcga gggccggcaa agcgatactg ttcggaccct acatggccaa
     1981 tatgcaggcg gttgccgagg cgttcaaagc tcaaggcgcc gccgtcgaag tcgccgacgg
     2041 cgttgccttg tcc
//
LOCUS       DASQKS010000188         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_518913, whole genome shotgun sequence.
ACCESSION   DASQKS010000188 DASQKS010000000
VERSION     DASQKS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518913"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VE170_04890"
     CDS             <1..147
                     /locus_tag="VE170_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017135477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ14802.1"
                     /translation="IMTSKALLDKNPKPTDAQIKEALAGNLCRCGTHVRILRAVKRAA
                     GQLA"
     gene            158..2434
                     /locus_tag="VE170_04895"
     CDS             158..2434
                     /locus_tag="VE170_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ14803.1"
                     /translation="MKSKKTSTLTRRQFLKSSGALIVSFNLFPPSGVRAQSNVEPHFD
                     ADPTQLDSWLAIAPDGTVTVYSGKVDLGTGVETALAQIVAEELDVPFNRIHMIGLDTA
                     KAIDQGITAGSRTIERGGPQLRQAAAAARQELLKLAATRLNAIVEKLTVTDGVVSDGK
                     SSVSYGQLIGGKKFNLKITAEGTGWNMKVAPEVRAKNVKDYKIVGTSQKRLDLPPKLT
                     GEFVYAHDVRIPGMLHGRVVRPPTITSKPANIDENAVKNIPGLVKVVQEGSFVGVVCT
                     TEWAAVKAAKTLKVTWSQPATKMPANYDELFGYIKSTKAYNDQVRVNRGNPEQALKQA
                     SKIYEATYRWPFQLHGMMGPSCSVADVNGDQVTLYTGTQGSFETREAVAQLLGMPEKN
                     VRVLWREAAGSYGRLGADDAAEDAALLSRAVGKPVRVQWMRDDEHAWDPKGPAQLGLA
                     RAGVDDQGKVIAWDYADRGFPLTAASGRGLRLLASRQIGMKATGTGNSNGSQGVGDMY
                     TFENQKGSAPLVPWILPAEETPLRTGYLRAPGDIARCFGSECFIDEIATDQKVDPVQF
                     RLRYLADNKRGIDALNAVVKQARWQERPSPAPATTGNTATGRGVSISNRANAICGAVA
                     EVEVDKSNGNVRVTRFVLAHDCGLIINPDGLKNQIEGNIIQGVSRSLLEEVKFDASGV
                     TTLDWVSYPVIRFPDVPEIELVLINRPEMPALGGGEPSSAPIAAAIGNAIFDAVGARL
                     REAPFTPKRVLAAMQKKA"
     gene            2547..3521
                     /locus_tag="VE170_04900"
     CDS             2547..3521
                     /locus_tag="VE170_04900"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HYJ14804.1"
                     /translation="MNQVADRCKGVIFGHAIGDALGLGAEFRPKATVAANYPNGLDRY
                     SQIIQDPHRRRWAAGDWTDDTDQMLCIFDSILEGQDVSILDIAKRIRAWVAEDGMGIG
                     NTVWSVLQSRYFLSAPHLAAQYVWESTGRHVASNGGVMRTSILGVWRYDSLAEVKKNA
                     AKVCRITHYDPRCVGSCVAVSTAIALLLQGKEAFSEILEIVTHESEGYSPELDKYLEM
                     AATGTLDDFDLDEGLTATELGSIGYTLKAMGAGFWALKNAPSYRDGVLQVIHEGGDAD
                     TNAAIVGALLGAKFGFANIPAIWVTELRDANELDARVTRMLGLLGLVK"
BASE COUNT          803 a         1085 c         1034 g          652 t
ORIGIN      
        1 atcatgacgt cgaaagccct gctcgataag aatcccaagc cgacggatgc acagatcaag
       61 gaagctctgg cggggaatct ctgccgctgc ggcacccatg taagaatcct gcgcgcggtc
      121 aaacgcgcgg cggggcaact agcttaagga gccgatgatg aaatcaaaga aaacttcgac
      181 tcttactcgc cgacaatttc tcaaatcctc gggcgcgctg atcgtcagct tcaacttgtt
      241 tccaccatcg ggcgtgcgcg cgcagtcgaa tgtggagccg cactttgacg ccgacccgac
      301 acaactggat tcctggctcg ccatcgcgcc cgacggtacg gtgacggtct attccggtaa
      361 agtcgatctc ggcaccggcg tggaaaccgc gctggcgcaa atcgtcgccg aagaattgga
      421 cgtgccattc aaccgcatcc atatgatcgg attggacacg gcgaaagcca tcgaccaggg
      481 catcaccgcc ggcagccgaa ccatcgaacg cggcggcccg cagctgcgcc aggccgccgc
      541 cgcggcacgc caggagttgt tgaaactagc cgccacccgg cttaacgcga ttgtcgaaaa
      601 gctcacggtc acggacggcg ttgtcagcga tggaaaatca tccgtatcct acggccagct
      661 catcggcggc aagaagttca acctaaaaat caccgccgaa ggcaccggct ggaacatgaa
      721 agtcgcgccc gaggtgcgcg cgaaaaacgt caaggattat aaaatagtcg gcacttcgca
      781 gaaacggctc gatctaccgc cgaagctgac tggtgaattt gtctacgccc acgatgtccg
      841 catccctggc atgttgcacg gccgcgtggt gcggccgccc acgataacct ccaagccggc
      901 gaacatcgat gaaaatgccg taaagaacat cccaggtttg gtgaaagtcg tgcaagaagg
      961 aagttttgtc ggcgtcgtct gcacgacaga atgggctgca gtcaaggcgg cgaaaacgct
     1021 taaagtcaca tggtcgcaac cggcgacgaa gatgccggcc aactatgatg aactcttcgg
     1081 ctacatcaaa agcaccaaag catataacga tcaggtccgg gtcaatcgcg gcaatcccga
     1141 gcaagcactg aaacaggcga gcaaaatcta cgaagccacc taccgctggc cgtttcagtt
     1201 gcacggaatg atgggtccat cctgctctgt ggctgacgtc aacggcgatc aagtaacact
     1261 ctacaccggc actcaaggtt ccttcgagac tcgcgaagcg gtggcgcaat tactcggcat
     1321 gccggaaaaa aatgtccgtg tcctctggcg cgaagcagca ggcagctacg gccgcctggg
     1381 tgcggacgat gccgccgaag acgccgcgct gctgtcgcgt gcggtcggca aaccggtgcg
     1441 tgtgcaatgg atgcgcgacg acgaacatgc ttgggatccc aaaggcccgg cgcagctggg
     1501 tctagcacgc gccggcgttg acgaccaagg taaagtaatc gcttgggact atgccgatcg
     1561 tggctttccg ctcacggccg cttccggccg cggcttgcgt ctgctcgctt cgcgccagat
     1621 cggcatgaaa gccaccggca cgggcaactc caacggcagt caaggtgtcg gtgacatgta
     1681 taccttcgag aatcaaaaag gcagcgcccc gttggtgccg tggatattgc cagccgaaga
     1741 aacgccactg cgcactggct acttgcgcgc gccaggcgac atcgcgcgtt gcttcggtag
     1801 cgaatgcttc atcgatgaga ttgccaccga tcagaaggtc gacccggtgc aatttcgcct
     1861 gcgttatctt gccgacaaca aacgcggcat cgatgctttg aacgcagtcg ttaaacaagc
     1921 gcgttggcaa gagcgtccct cgcccgctcc cgcaaccacg ggaaacacag caaccggccg
     1981 cggcgtgtcg atctcgaacc gcgctaacgc gatctgcggc gccgtcgccg aagtcgaagt
     2041 cgacaaatcc aacggcaatg tccgggtcac gcgatttgtt ctggcccatg actgcggcct
     2101 gatcatcaat cccgacggac tgaagaacca aatcgagggc aacatcattc agggcgtcag
     2161 ccgctcgctc ctggaagagg tcaagttcga cgcctcgggc gtcacgacgc tggactgggt
     2221 cagctatccg gtgatccgct ttcccgacgt gccggagatc gaactggtgt tgatcaatcg
     2281 cccggagatg cccgccctcg gcgggggcga gccctctagc gcaccgatcg ccgcggcgat
     2341 tggcaatgcc atcttcgacg cggtcggcgc gcgcctgcgc gaagcaccgt tcacaccgaa
     2401 gcgggttttg gcggcaatgc agaagaaggc atgagatgat gcccggggcc ggcgattcat
     2461 cgccttgcaa ccatcgccgg ccccgtttgc tgccaatagc ggcttgtcat cttaactcgc
     2521 atcatagaaa caccagccca gggaacatga atcaagtcgc ggaccggtgc aaaggagtaa
     2581 tcttcggtca cgccatcggc gacgccttgg ggctgggcgc cgaattcagg ccgaaagcga
     2641 cggttgccgc caattacccc aacggcctcg acaggtattc acaaataatt caggatccgc
     2701 atcgccgacg ctgggccgcc ggcgattgga ccgacgatac cgaccagatg ctgtgcattt
     2761 tcgacagcat tctcgaagga caagacgtca gcatcctcga catcgccaaa cgcatccgtg
     2821 catgggtggc tgaggatggc atgggcatcg gcaataccgt ctggtcggtg ctccagtcga
     2881 gatattttct ctccgcgccc catttagccg cccagtacgt ttgggaatca accggccggc
     2941 atgtcgcatc caacggcggg gtcatgcgca cgtcaattct cggcgtgtgg cgatatgact
     3001 ccttggcgga agtcaaaaag aatgccgcga aggtctgccg tatcacgcac tacgacccgc
     3061 gctgtgtcgg ttcgtgcgtc gcggtcagca cggcgatcgc cctgctgcta cagggcaagg
     3121 aggcattctc cgaaatatta gaaatcgtca cccacgagag cgaaggatat tcgcctgagc
     3181 tcgacaaata tctggaaatg gcggcgaccg gcacccttga cgactttgat ctagacgaag
     3241 ggttgaccgc cactgaactt ggctcgatcg gctataccct caaagccatg ggcgccgggt
     3301 tttgggcgct caaaaatgcc ccgtcctatc gcgatggcgt tttgcaggtg attcatgaag
     3361 gcggcgatgc agataccaac gccgccatag tcggcgccct actcggcgcc aagttcggct
     3421 tcgccaacat cccggcaatc tgggtcacgg aactgcgtga cgcaaacgag ctggacgcgc
     3481 gagtaactcg aatgcttgga cttttgggac tcgtgaaata accccgtctc ggcgccaaca
     3541 cgccatttgc ccttgacgtt tttgccttga cgcc
//
LOCUS       DASQKS010000189         4290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5136661, whole genome shotgun sequence.
ACCESSION   DASQKS010000189 DASQKS010000000
VERSION     DASQKS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5136661"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VE170_04905"
     CDS             <1..452
                     /locus_tag="VE170_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ14805.1"
                     /translation="EAAQAASHPLIPARAVLTTGKRLSVVLVDDHDVVRWGLRQILDE
                     FADLTVVGEAKDGKAGVELARRLKPDVVVMDINMPRMNGIEATKLITQELPSTIVVGL
                     SFDIGAEITQTMKDAGAFTCVNKERAVENIYQAIKDAVEDRRETAAE"
     gene            complement(664..1296)
                     /locus_tag="VE170_04910"
     CDS             complement(664..1296)
                     /locus_tag="VE170_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14806.1"
                     /translation="MNHRLNRQFFGQIFANVALAGLVAINVSCSAIDTKPATLDERGK
                     LKEKIDQVIANGALDPVPAKSTGFTEAEINTILNSFAGDWIPNGISEPQLRLLGNARI
                     SLRVIVDIDEFKRRRRRPGGAGPLNFFSGKMPALVRGDLHTGDGKGQFRLHSTEVNGI
                     PLPRDLALELLSTHSKTRRNPNGFDLEKPFDLPANIRQLQIFPTEIVVIQ"
     gene            1523..1690
                     /locus_tag="VE170_04915"
     CDS             1523..1690
                     /locus_tag="VE170_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14807.1"
                     /translation="MIFDLITYILLGSIFVVNTLRMFFEKPKNILQSAPLPWQRLLGR
                     RSNAGSKANGF"
     gene            complement(1749..2705)
                     /locus_tag="VE170_04920"
     CDS             complement(1749..2705)
                     /locus_tag="VE170_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14808.1"
                     /translation="MLILIAPRPTYAQNPVMIGVAGPAVNLIYAYLAQDTGLWRKYGV
                     DARVVLFESGSTLAQVARSGEVSFAINSGPTTIASRSQGADTIIIAAMVNTLPYSVVA
                     AKSITTWNQLKGKKVGISRFGSGTDTAMRLVVKRYGLDPVKDLVILQGGTQPSRLQAV
                     AAGSLDATLVSPPLDLTAKKQGLSILVNIADLGIPYPQLVIETTERFNREQPLVVKNF
                     LKGFIEAVHYAATHKDETKKTITKYLKTNDPEILEATHKSFLQVTDYSAMPNLEGIRN
                     AMDEVATRLPAVKNKKPEDFVDTRFLKELEKEGFFKQLQKKN"
     gene            complement(2902..3327)
                     /locus_tag="VE170_04925"
     CDS             complement(2902..3327)
                     /locus_tag="VE170_04925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ14809.1"
                     /translation="MNKIDYRYGNNLDLDQVVELYRASTLGERRPVDNRATMKNMLEH
                     ANLVVTAWDGEVMVGIARALTDFAYVGYLADLAVRASHQRHGIGVKLIHQTRARMGPR
                     AMLVLLAAPKAVDYYPKIGFTQHGSAWILNATDSLLDRY"
     gene            3446..>4290
                     /locus_tag="VE170_04930"
     CDS             3446..>4290
                     /locus_tag="VE170_04930"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYJ14810.1"
                     /translation="MADNLKPRSAVITDGPDRAPTRAMLKAVGFSDEDLRRPIIGVAN
                     TWIEIGPCNYHLRRLAVKVKEGIRAAGGTPMEFNTVSISDGISMGVEGMKCSLISREV
                     IADSIELVARGNHFDGLVCLSGCDKTNPGVVMALARLDIPGLALYGGSIAPGHLADKD
                     LTIQDVFEAVGAYASKRIDAEQFLAVENAACPGAGACGGQFTANTMSTVMEFLGISPM
                     GSNGIPATVAEKDQAAFNAGRLVMDLLKRDLRPSMILTRKSIENAIASVAATGGSTNA
                     VLHLLA"
BASE COUNT          919 a         1136 c         1247 g          988 t
ORIGIN      
        1 cagaagcagc gcaggcggcg agccatcctc ttataccagc gagggcggtg ctgacgacgg
       61 gaaaacggtt aagcgtggtg ctggtggacg atcacgatgt ggtgcgctgg ggcctacggc
      121 aaatactcga tgagttcgcc gaccttacag tagtcggcga agccaaagac ggtaaagcag
      181 gcgtcgagtt ggcgcgccgg cttaaacccg acgtggtcgt gatggatatc aacatgccgc
      241 gcatgaacgg catcgaagcg accaagttaa ttacccagga gctgccgtcg acgattgtcg
      301 ttgggctgtc ctttgatatc ggagccgaga taacccaaac gatgaaagac gctggcgcct
      361 tcacctgcgt taacaaggaa cgggcggtgg agaatattta ccaagcgatc aaggacgcgg
      421 tggaggaccg gcgggagacg gcggcagaat aacctcggca tgtttgtcat agcgcaccga
      481 caaagtattc cggcagccga aaaaattccc ctcgccaaag caggaccgaa tctcttcacc
      541 gaaacggtct gacttgttga cagctcgcgc cgcgagaaat ctcatttagg ttctttaaat
      601 taaccgggat gtcgggcatt ggcctctact ttggcggcga atgcatcaac ctcatcggcg
      661 cgcttactgg atgacgacga tctccgtcgg aaagatctgt aactggcgaa tgttggcggg
      721 caggtcaaat ggtttttcca gatcgaaacc gttggggttg cgtctggttt tactgtgggt
      781 cgatagcagt tccagcgcca ggtcgcgcgg cagcggaatt ccgtttacct cggtcgaatg
      841 caatctaaat tgccccttgc catccccggt gtgtaaatcg ccgcgcacta gagcaggcat
      901 cttgccgctg aaaaaattga gcggtccggc accgccggga cgccggcgcc ggcgcttgaa
      961 ctcatcgatg tcgacgatga ctcgtaggga gattcgcgcg ttaccgagaa gccgcaattg
     1021 gggctcgcta ataccgttgg gaatccagtc tccggcaaag ctgttcaaga tggtattgat
     1081 ctctgcttcg gtgaagccgg tggattttgc tggcaccgga tcgagggcgc cattggcgat
     1141 cacctggtcg attttttctt tcagcttgcc gcgttcgtcg agggttgccg gtttggtgtc
     1201 gatcgctgaa caactgacgt tgatcgcgac cagcccggcg agcgcgacat tagcaaaaat
     1261 ttggccgaag aattggcggt ttagtcgatg attcactgcg tcagctgcgt ccgaaactgc
     1321 cgatcgaaaa tacacggatt agacgtcaac ggttggggca gtgctctttt ttgcaccgcg
     1381 ctgacctgag aactgcctag aaatccaccc acgccggttt cccatcaatc caaaaccttt
     1441 gaaattcaac tacttacata agttaatccg tggcttggct cttgctccgt agctggagag
     1501 ctgagctatt caggaggcaa gcatgatttt cgatttaatt acctatattt tgttaggcag
     1561 catcttcgtc gtcaatacgc tgcgcatgtt ttttgagaaa ccgaagaaca ttttgcaatc
     1621 ggctccgctg ccgtggcagc gattgttggg gcggcgctcc aatgcaggat ccaaagcgaa
     1681 cgggttttaa ctcaccgacc aatccggcgg tctcctggtg tctgcgccgt ctgaacccgc
     1741 ccgccggatc agttcttctt ttgcaactgt ttgaagaagc cttccttctc cagttccttt
     1801 aagaatcggg tatcgacaaa gtcctcgggt tttttatttt tcaccgcggg caggcgggtg
     1861 gcaacttcgt ccatcgcatt gcgaatgcct tccaggttgg gcatcgcgct gtaatcggtc
     1921 acttgcaaga aactcttgtg cgtcgcttca agaatctccg gatcgttggt cttgagatac
     1981 ttggtgatgg tcttcttggt ttcgtcttta tgcgtagcgg cgtagtgcac tgcttcgatg
     2041 aagcccttca agaaattctt aaccaccagc ggttgttcac ggttgaagcg ctcggtggtc
     2101 tcgatcacca actgcggata ggggatgccg agatcggcga tgttcacgag gatcgacaat
     2161 ccttgtttct tcgccgtcag gtcgagggga ggagagacca aggttgcatc gagagagccc
     2221 gccgcaactg cttgcaaacg gctcggctga gtaccgcctt gtaaaattac caaatccttc
     2281 accgggtcta gaccgtagcg tttgaccacc aggcgcatgg cggtatcggt gccggagccg
     2341 aaacggctga tgccgacctt tttgcccttg agttggttcc aggtggtgat cgacttggcg
     2401 gcgactacgc tgtagggcag tgtgttgacc atcgccgcga tgatgatggt gtcggcgccc
     2461 tggctgcgcg aggcgatggt ggtgggaccg gaattgatcg cgaaactcac ttcgcctgag
     2521 cgggccactt gggccagggt ggagcctgat tcgaatagca ccactcgcgc atcgacacca
     2581 tatttgcgcc atagaccggt atcttgggcc aagtaagcgt aaatcagatt gacagccgga
     2641 ccggcgacgc cgatcatcac cgggttttgg gcgtaggtcg gacgcggcgc gattaagata
     2701 agcacggcta gagtaaggga ttgaaaaaac tgtctagtag aattcctcac atgttctccc
     2761 tatgaatatt cgctagcaac cactccggtc tactcctagt caaatgagca cctctgtcgc
     2821 tcggtggcag aagcgtggca aattcgtaac agtttagcga aataccgcag cttctgaaag
     2881 aaccaatcgt ataccgccaa atcaataccg gtctagtagg gaatcggtgg catttaggat
     2941 ccaggcgctg ccgtgttggg tgaacccgat cttcgggtaa taatcgacgg ctttgggtgc
     3001 ggcgagcaac accagcatgg ctcttggccc catacgtgcg cgggtttggt gaatgagttt
     3061 aacgccgatg ccgtgccgct gatgcgacgc gcgcacagcg agatcggcca gatagccgac
     3121 gtaagcgaaa tcggtcagcg cgcgagcgat cccgaccatc acttcgccat cccacgcggt
     3181 gaccaccagg ttggcgtgct ccaacatatt tttcatggtt gcccgattat ccaccggacg
     3241 acgctcgcca agtgtcgagg cacgatagag ctcgacgacc tggtcgagat cgaggttgtt
     3301 gccgtaacga taatctattt tattcataat tcggcctttt catgcgctcc ggttcgatat
     3361 ctaagtcgag tccgctctgc ttgcaaatcc ccaggtgcca tagttaattg acagtctcac
     3421 gtttgattag ggttaggaga cattcatggc agacaatctc aagccgcgca gcgcagtgat
     3481 taccgatggt cccgaccggg cgccgaccag ggcgatgctc aaggcggtgg gtttttccga
     3541 cgaagattta cgccggccga tcatcggcgt ggctaatacc tggatcgaaa ttggcccgtg
     3601 caactatcac ctgcgccgtt tggcggtcaa agtgaaagag ggcatccgcg ccgccggcgg
     3661 cacgccgatg gagttcaata ccgtgtcgat ctccgacggt atcagcatgg gcgtcgaagg
     3721 catgaagtgc tcgttgatta gccgcgaggt gatcgccgat tcgattgaat tagtcgcacg
     3781 cggcaatcat ttcgacggtt tagtttgcct ctccggttgc gataaaacca atcccggcgt
     3841 cgtcatggca ctcgcgcgcc tcgatattcc cggtctggcg ctttacggcg gttccatcgc
     3901 gccgggccat ttggctgata aagatctgac cattcaagat gttttcgaag cggtcggcgc
     3961 ctacgcgagc aagcgcatcg acgccgagca gtttttggcc gtggaaaacg ccgcctgtcc
     4021 aggcgcgggc gcctgcgggg gtcagttcac cgccaacacc atgtcgacag tgatggaatt
     4081 tctcggcatc tccccgatgg gcagcaacgg gatcccggcg acggtggcgg aaaaagatca
     4141 agcggcgttc aacgccggta ggctggtgat ggatttactc aagcgcgatc tgcgtccgtc
     4201 gatgattctt acgcggaagt cgattgaaaa cgccatcgcc tcggtggcag ccactggtgg
     4261 atcgaccaac gcggtgttgc atttgctggc
//
LOCUS       DASQKS010000190         8171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4719923, whole genome shotgun sequence.
ACCESSION   DASQKS010000190 DASQKS010000000
VERSION     DASQKS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4719923"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VE170_04935"
     CDS             <1..992
                     /locus_tag="VE170_04935"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HYJ14811.1"
                     /translation="ASDLALVDAGLAGDPLVQSGHLGIAYGSSAGTPKAICDFGLMIS
                     EKSTKGINATTYIKMMGHTAPVNLGVFLGVTGRIITTSSACTSGSQGIGYGYETIRAG
                     QQRVMIAGGAEELCVTEVAVFDTLFATSVRNDAADTTPKPFDIDRDGLVLGEGAGTLI
                     LEEFDHARARGAPVYAEIVGFGTNSDGCHVTQPNAKTMQVAMELALADANLPPSAIGY
                     VNAHGTGTQHGDVAESLATGKLFGSQVPVSSLKSYMGHTLGACGALEAWMSIEMMRTG
                     WFAPTINLARVDPACGELDYIVDQGRVLDCEYVMSNNFAFGGINTSLIFRRMA"
     gene            1106..1552
                     /locus_tag="VE170_04940"
     CDS             1106..1552
                     /locus_tag="VE170_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ATPase subunit"
                     /protein_id="HYJ14812.1"
                     /translation="MKGIYHLTIIVALTLSAAWPAHARDSKLMFPIAPALEAKDAKEK
                     LDGSVKFYFGNQETPKILTKLGTDVTNQKTNAVGKSDERACNWVFLSAMIQLDKRAKQ
                     LGANAVVNIVSYYQKSVMSSPTEFECHAGNIVAGVALRGEFVKIAE"
     gene            1822..2079
                     /locus_tag="VE170_04945"
     CDS             1822..2079
                     /locus_tag="VE170_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14813.1"
                     /translation="MGNVTPHGRASTRRTDDVNACNAAFLAAALVDFQKRAKMAGANA
                     VINIVSYYKNVEMASPTEFECHAVAAAHALLRGDMVKIAAE"
     gene            complement(2122..2706)
                     /locus_tag="VE170_04950"
     CDS             complement(2122..2706)
                     /locus_tag="VE170_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ14814.1"
                     /translation="MTFYQKNLAATLAVFACSLMPIANTDADYPEEIPFVPTPMDVVD
                     QMLELAEVKKGDVVYDLGSGDGRIVIRAAKKYGVRAVGIEMDRLLLDKARKDAKAAGV
                     GHLVEFRAEDAMKVDLTKATVVTLYMLPWFNEAMKPSFKKYLKSSARIVAHDFGIEGW
                     EPDKTVKLPDPEKKIGGLIHYHTLFLWRIGKNDI"
     gene            2858..4183
                     /locus_tag="VE170_04955"
     CDS             2858..4183
                     /locus_tag="VE170_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14815.1"
                     /translation="MENDISATPKKRPPISLPFYYGWFIVSLCFLTTLTSAGVRSSPQ
                     VLILPLQLEFGWSRALIASGISMNLLLFGVAAPISGWLIDRFGPRKVMLGSLILLITG
                     VSGTVAMTEFWQFFLVWGVIVGLGAGGVGSVLTATVGNRWFVARRGLALGILGSASST
                     GQLIFLPFFMWMIASSGWRIGSTVLIILAILLLPLLYLFMRDDPADVGLEPYGAGQTN
                     VGGGAASLRGMSSKNATITAREVVTNPTFWLLCSTFFVCGGTANGLIGTHLIPHEIEI
                     GIPQIAAASLVGIMGTLNIVGTTFSGWMVDKVSPHKWLALVYALRGVSLLILPFVKSF
                     TGLVIFAIIYGLDWFATVPPSMAITADTFGRQNVGKVYGWIFMSHQIGAAIMASGAGA
                     VRDYLGEYNAAFLAGGFIAMIAAALALQIKPKPKESALPPPATAQPAGA"
     gene            complement(4194..4604)
                     /locus_tag="VE170_04960"
     CDS             complement(4194..4604)
                     /locus_tag="VE170_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14816.1"
                     /translation="MKTSQICHMRAALVALLLIAACGRDLNTAQGVAEEFVDHHYVKI
                     DLQKAKQYTVSVAQAKIDEEIRLTKGQTIDASTQTPRVNYALLSKNEGEQRSTYLFEG
                     KIQSDDGTSFIRKWSIATRKETNGWRVSNFTETD"
     gene            complement(4614..5369)
                     /locus_tag="VE170_04965"
     CDS             complement(4614..5369)
                     /locus_tag="VE170_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ14817.1"
                     /translation="MAIEEELSLRTVNGDTLSAVLHHPAHASYGAAILCHGMESAKKS
                     EKLIFLAQALAERGIPALRFDFRYVGASSGKFADITYSGEVEDLKTAYDFVQSRRLGK
                     TVLFGSSMGGTVALLFAAQEPDIAAIVTLAAPVHPENFPTRLLTPTQLEHWRERGYIT
                     YNGRRLNVSLLADLDTLDVLHRTQQVRCPVLILHGDADEVVPVAEAYELHDCLPGPKR
                     LSIFTGIDHRFSNPLVIQQAMSEALDWLTKHVR"
     gene            5477..6385
                     /locus_tag="VE170_04970"
     CDS             5477..6385
                     /locus_tag="VE170_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HYJ14818.1"
                     /translation="MESLIQQYAGYLRNQRNVSPHTLRNYLSDLGQFQSFLADRQLAL
                     AADGSMDAGKIDIHVVRAYLASLAPDRKKSSIGRKLAALKGFFRYLVTTKRLGKDPLL
                     LINSPKQEKPLPKFLTVDDMFHVLDTIKLASGLDQRDRGVLEVLYSTGIRVSELVGLN
                     WSDIDIQLGIIRVVGKGSKERIVPIGAVALKALESYADWVHQQWQVACRGATPVFLNH
                     RGRRITTRSVARIVDKHLRLAGIQIKMGPHGLRHSFATHLLNSGADLRVIQELLGHVS
                     LSTTQRYTHLNLDQLTAVYDKAHPRA"
     gene            6442..6969
                     /gene="hslV"
                     /locus_tag="VE170_04975"
     CDS             6442..6969
                     /gene="hslV"
                     /locus_tag="VE170_04975"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HYJ14819.1"
                     /translation="MMHGTTILAIRHGGKVVVVGDGQVSLGQTVMKHSARKVRKLHHD
                     KVIAGFAGATADAFTLFEKFEAKLEQFNGNLKRAAVELAKDWRTDRILRRLEALLIIA
                     DATDLLVISGAGDVIEPDDGVIAIGSGGNYALAAARVLVKHTQLDARVIAEEAMRVAA
                     GICVYTNEQLTFEEL"
     gene            7122..>8171
                     /gene="hslU"
                     /locus_tag="VE170_04980"
     CDS             7122..>8171
                     /gene="hslU"
                     /locus_tag="VE170_04980"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HYJ14820.1"
                     /translation="MNDVAKSLNDAGTSPAPVMTPREIVSELDRYIVGQKDAKRAVAI
                     ALRNRWRRQLVPDDLRDEIAPKNIIMIGPTGVGKTEISRRLAKLAQAPFIKVEASKFT
                     EVGYVGRDVESIIRDLTDLAVNMVKAEEKQKVEIRAREVAEERVLDLLLPAGEAVDAE
                     AVDAARLQGTRDKLKQMLRDGKMDDRFVEIEMSQSVMPMIEVLTPQGMEGMEFNLKDM
                     FSNMMPKKTKKKTVKIPEALEILTQEEAGKLVDMDKVTGEATRRVEQSGIVFLDEIDK
                     IAGRESAHGPDVSRQGVQRDLLPIVEGCTVNTKYGIVRTDHILFVASGAFHTAKPSDL
                     IPEFQGRFPIRVELSS"
BASE COUNT         1700 a         2241 c         2403 g         1827 t
ORIGIN      
        1 gtgccagcga cctggcgctc gtggacgccg ggctcgccgg tgatccgttg gtgcagagcg
       61 gtcacctcgg tatcgcctat ggttcttccg cggggacgcc gaaagcgatt tgcgatttcg
      121 gcttgatgat tagcgagaaa agcaccaaag gcatcaacgc caccacttac atcaaaatga
      181 tgggccatac cgcgccggtc aatcttgggg tttttcttgg cgtcaccggc cggattataa
      241 cgacttcgag cgcttgcacc tccggcagcc agggcatcgg ctatggctat gagaccattc
      301 gtgccgggca gcagcgggtg atgattgccg gcggcgccga agagctatgc gtcaccgaag
      361 tggcagtctt cgataccctg ttcgctacta gcgtgcgcaa cgatgccgcc gataccacgc
      421 caaaaccctt cgacatcgat cgcgatggtt tggtcctcgg cgaaggtgcc ggcaccctga
      481 ttcttgaaga gttcgaccat gcgcgagcac gcggcgcgcc ggtctatgcc gaaatcgttg
      541 gtttcggcac taacagcgac ggctgtcatg tcactcagcc caacgccaaa accatgcaag
      601 tggcaatgga gctggcgctt gccgatgcca atttaccgcc ctcggcgatc ggttacgtca
      661 atgcgcacgg tacgggcacg caacatggcg atgtcgccga atcgctggcg accggcaaat
      721 tgttcggctc ccaggtgccg gtcagctcgc tcaagagtta catgggacat accctcggcg
      781 cctgcggcgc gctggaagcg tggatgtcga tcgaaatgat gcgcaccggt tggttcgccc
      841 cgacaataaa tctggcacgc gtagatcccg cctgtggcga gctcgattac atcgtcgatc
      901 aaggccgcgt gctcgactgc gaatatgtca tgtcgaacaa tttcgcattc ggcggcatta
      961 acacctcgtt gatctttcgc agaatggctt aggaaatttt accggaggct gtcagaatga
     1021 aatcccgagt ctgttcaaag aagttgccgt acaggtcgtt gaaagtttat agtctaagtc
     1081 taaaatccgg aaggagagag gatcgatgaa aggaatttat cacttaacga ttatcgtcgc
     1141 gttaaccttg agcgctgcct ggcccgccca cgcgcgcgat agcaaactta tgtttccgat
     1201 cgcccccgct ttggaagcca aggatgccaa ggaaaaactt gacggctcgg tgaagtttta
     1261 tttcggcaat caggagacgc cgaaaattct caccaagctc ggcaccgatg tgaccaatca
     1321 aaagaccaat gccgtcggta agtccgatga aagggcgtgc aactgggtgt ttctgtcggc
     1381 aatgattcaa ctcgacaagc gcgccaaaca attgggcgcc aatgccgtcg tcaatattgt
     1441 cagctattat cagaagagtg tcatgtcaag cccgacggag tttgagtgcc atgccggtaa
     1501 tatcgtcgcc ggagtcgccc tgcgaggtga attcgtcaag attgcggagt agttcggtat
     1561 cgccctcaaa gtgacggtcg tcggagcggt gggcgaaagc agattttaac cgtcctcatg
     1621 gcttaaggct ttgaaattaa tacgcgtctc aacccttggg ctggcggtat ctcgcgccgt
     1681 cgcctgccct gcccaggcca ggaacgataa atttctgctg ccgatcaaag cggcgctggc
     1741 atcgactgaa gctcatgaaa aacccgaagg gtctgtcaag tttttcttcg gcagcagccg
     1801 acgccggcga tcgtcacaaa cttggggaat gttacgcctc acggcagagc gagcacccgc
     1861 cgtactgacg atgtcaacgc gtgcaacgcc gcgttcctag cggccgccct ggtcgatttt
     1921 caaaaacgcg ccaagatggc gggcgccaat gccgtgatca acatcgttag ctattataag
     1981 aatgtcgaga tggcgagccc gaccgagttc gagtgtcacg ccgtggccgc cgcccatgcc
     2041 ctattgcgtg gcgatatggt caagatcgcc gctgagtgat ccagctaaat cacgccaatc
     2101 cacagggatc gggtcacgat actaaatgtc attcttgcca attctccaca aaaacaacgt
     2161 atgatagtgg atcaacccgc cgattttttt ctcggggtcg ggcagcttta ccgttttgtc
     2221 cggttcccag ccttcgatgc caaaatcatg ggcgacgatg cgggcgctgg atttcaaata
     2281 tttcttgaag cttggtttca tcgcttcgtt gaaccagggg agcatgtaga gagtgacgac
     2341 ggtggccttg gtgagatcga ctttcatggc gtcttcggcg cggaactcga ccaggtggcc
     2401 aaccccggcg gctttggcgt ctttacgcgc tttatcgagg agaagccggt ccatttcgat
     2461 gcccaccgca cggacaccat attttttcgc ggcgcggatg acgatgcggc cgtcgccgct
     2521 gccgagatcg taaaccacat cgcctttctt gacctcggcg agctcgagca tttggtcgac
     2581 gacgtccatc ggcgtcggca cgaaagggat ttcttcaggg tagtcggcat cggtattggc
     2641 aattggcatc aatgagcaag caaatacggc aagggtggca gccaagtttt tttggtagaa
     2701 tgtcatttgg ccatttgttc ctttcacagt tcgatgattg acgtattggg aaaaactacc
     2761 tgatcgcgct ccgagctgtc aatttaccgt ccttgcccta atactcaaca gcagaccggt
     2821 cgtttgtggt tcgagctcag atcgggctat atgaagtatg gaaaacgaca tcagcgctac
     2881 tcccaagaag cgtccgccga tctcgctgcc tttttactat ggttggttca ttgtgtcgtt
     2941 gtgttttttg accactctaa cgagcgcagg cgtgcgctcg tcgccgcaag tcttgattct
     3001 tccgctgcag ctggagttcg gctggagccg ggcgttgatc gcttcgggta tcagcatgaa
     3061 tctgctgctc ttcggcgtgg ccgcgccgat tagtggctgg ctaatcgacc gcttcgggcc
     3121 gcgcaaggtc atgctcggca gcctgattct attgatcact ggagtcagcg gcacggttgc
     3181 catgaccgag ttctggcaat tttttttggt ttggggcgtc atcgtaggtc tgggggctgg
     3241 cggcgtgggc tcggttttga ccgccacggt aggaaatcgc tggtttgtcg cgcggcgcgg
     3301 actagcgttg ggaattctcg gcagcgccag ttcgacgggt cagctgatat ttctgccgtt
     3361 cttcatgtgg atgatcgcca gctccggatg gcgcatcggt tcgacggttt tgatcattct
     3421 cgcgatcctc ttgttgccgt tgctgtatct tttcatgcgc gacgatccgg ccgatgtcgg
     3481 cttggagccc tacggcgccg gacagaccaa tgtcggcggt ggcgcggcct cgctgcgcgg
     3541 catgtcctcg aagaatgcca cgatcacggc gcgcgaagtc gtcaccaacc cgacattttg
     3601 gctgctgtgc agcaccttct tcgtctgcgg cggcacggcc aatggtttga tcggcacgca
     3661 tttgatcccg catgaaatcg aaatcggcat cccgcagatc gctgccgcct ctttggtcgg
     3721 catcatgggc acgctcaaca tcgtcggcac gaccttttcc ggctggatgg tcgataaggt
     3781 gtcgccgcat aaatggttgg cgctggttta cgcattgcgc ggcgtgtcgt tactcatttt
     3841 gcccttcgtc aagagcttta ccggcttggt cattttcgcg attatctatg gtctcgactg
     3901 gttcgccacc gtgccgccgt cgatggcgat caccgccgat accttcggcc gacaaaatgt
     3961 cggcaaagtc tacggctgga tttttatgtc gcatcagatc ggcgccgcga tcatggcatc
     4021 gggggccggc gcggtgcgcg actatctggg cgagtataac gccgccttct tggcgggcgg
     4081 cttcatcgcg atgatcgcgg cggccttagc gttgcaaatt aagcccaaac ccaaggaatc
     4141 tgcgctgccg ccgccggcga ccgcgcagcc agccggggct tagcggattt ttgttagtct
     4201 gtttcggtga agtttgaaac gcgccagcca ttggtttcct tgcgcgtcgc gatggaccat
     4261 ttgcggataa acgaggtgcc gtcgtcggat tgaatcttgc cttcgaacaa ataagtcgac
     4321 cgttgctcgc cttcgttctt tgacaatagc gcataattta cccgcggcgt ttgtgtcgag
     4381 gcgtctatgg tctgcccctt ggtcaagcga atctcctcgt cgatcttagc ctgggcgacg
     4441 ctcacggtgt actgtttggc cttttgtaag tcgattttga catagtgatg atcgacaaat
     4501 tcctcggcga cgccttgagc cgtgtttaaa tcacggccgc aggcggcaat caggagcaaa
     4561 gccaccaggg cggctcgcat gtgacatatt tgtgatgttt tcatcgctgc gctttatcta
     4621 acatgtttgg tgagccaatc cagcgcttcg ctcatggctt gctggatgac cagcggattg
     4681 gagaaacggt gatcaatgcc ggtgaagatc gatagccgct ttgggccagg taggcagtcg
     4741 tgcagttcat aggcttcggc gaccgggacg acttcgtcgg cgtcgccgtg aaggatcagc
     4801 accggacagc gaacctgctg cgttctatgc agcacgtcga gtgtatccag gtctgcgagt
     4861 aaggacacat tcaagcgccg gccgttataa gtgatataac cgcgctcgcg ccaatgctct
     4921 aactgcgtgg gtgtcagcag gcgcgtcgga aagttttctg gatgaaccgg cgccgcaagg
     4981 gtgacgatgg cggcgatgtc gggctcttgc gcggcgaaaa gcaacgccac ggtgccgccc
     5041 atgctggagc caaagaggac agtctttcca agtcggcggc tttgcacgaa atcataggcc
     5101 gtcttaaggt cttccacctc gccgctgtaa gtaatatctg caaacttgcc gcttgatgcg
     5161 ccgacgtaac ggaagtcgaa gcgcagagct gggattcccc gctctgcgag cgcctgagcc
     5221 aggaaaatca atttttcgct tttcttggcc gactccatgc cgtggcacag gatcgccgcg
     5281 ccgtaagacg catgcgctgg atgatgtaaa accgcactga gagtatcgcc gttcactgtg
     5341 cgcaacgaaa gctcttcttc aatcgccaca gggtacgatt aacatgaatc gaaatcgacg
     5401 gaaacggcgg ctcacccatc aattggtaga gttcaactgt ggttggtggt attctaaacc
     5461 agttgaaagc ccgaccgtgg aatcgttaat ccaacaatat gccggctatt tgcgtaacca
     5521 gcgcaatgtg tcgccgcata ctctgcgcaa ctatctaagc gacttggggc aatttcaaag
     5581 ttttctcgcc gatcggcagc tggcattggc ggctgacggt tcgatggatg ccggcaagat
     5641 cgacattcat gtggtgcggg cttacctggc gtcattggcc ccggatcgca agaaaagttc
     5701 cattggccgc aaactggcgg cgttgaaggg gttctttcgc tatttggtga cgaccaaacg
     5761 gctcgggaaa gatccgctgt tgctgatcaa ctcgcccaag caggaaaagc cgctgccgaa
     5821 gtttcttacc gtcgacgaca tgtttcatgt gttggacacg atcaagcttg cgagcggcct
     5881 cgaccagcgt gatcgcggcg tgttggaagt gctttattcc acgggaattc gcgtcagcga
     5941 gttggtgggt ctaaactggt ccgacatcga catccaattg ggcatcattc gggtggtcgg
     6001 caagggatcc aaggaacgta tcgtgccgat cggcgcggtg gcgctgaagg cgctggaatc
     6061 ctacgccgac tgggtgcatc agcagtggca ggtcgcttgc cgcggcgcta cgccggtgtt
     6121 tctcaatcac cgcggccggc gcattactac gcgcagcgtc gcgcgtattg tcgacaaaca
     6181 cctgcggctc gcgggcatcc agataaaaat gggcccccat ggcttgcgcc atagctttgc
     6241 cacccacttg ctcaacagcg gcgccgattt gagagtgatt caggagctgc tcggccatgt
     6301 cagcctatcg acgacccagc gctataccca tttgaacttg gatcaactga ccgcggtata
     6361 cgacaaggcg catccgcggg cctagaaatt taaaattttt gattccgaat gggaatcgag
     6421 attcggcaag taccaacgaa tatgatgcac ggaacgacga ttctcgcgat acgccacggc
     6481 ggcaaagtgg tcgtggtcgg cgatggccag gtgagcttgg ggcagacggt gatgaagcat
     6541 agcgcgcgca aggtgcgtaa gctgcatcac gacaaagtca tcgccggttt cgccggcgcc
     6601 acggcggatg cctttacgct gttcgagaaa ttcgaagcca aacttgaaca attcaacggc
     6661 aatctcaagc gcgctgcggt ggagctggcc aaggattggc gcacggaccg tatcctacgg
     6721 cggttggagg cattgctgat catcgccgac gcgaccgatt tgctggtcat ttccggcgcc
     6781 ggcgacgtca tcgaaccgga tgacggcgtc atcgccatcg gctcgggcgg taactacgcc
     6841 ttggcggcag cgcgagtact ggtcaaacat acccagctcg atgcccgcgt gatcgccgag
     6901 gaggccatgc gggtggccgc cggcatctgt gtttacacca acgagcagct gacttttgaa
     6961 gagctttagt tcctcgtcgc gcgcaactgc aattccagat taaagcttcc cattccaaag
     7021 cgaggacgcg ggaccgagct cgtggatgtg gcctaccggc acgcttactc gctgggaaat
     7081 cattgcattt cctggcggaa ttgttaggag tgttgattcc tatgaatgac gtcgcaaaat
     7141 ctctcaatga cgccgggact tcgccggcgc cggtgatgac tccgcgggag atcgtctccg
     7201 agctcgaccg atatatcgtc gggcaaaagg atgccaaacg ggcggtggcc atcgcgctgc
     7261 gcaatcgctg gcggcgccag ctcgtgcccg acgatctgcg cgacgaaatc gcgccgaaaa
     7321 atatcatcat gatcggcccc accggcgtcg gcaagacgga aatctcccga cgcttggcca
     7381 agcttgccca ggcgccgttt ataaaggtcg aagcgtcgaa attcaccgag gtcggttacg
     7441 ttggccgcga tgtcgagtcg atcatccgcg atctgaccga tctcgccgtg aacatggtta
     7501 aagcggaaga aaagcagaag gtcgagatcc gggcgcgcga agtggccgag gagcgggtgc
     7561 tcgatttgtt gctgccagcc ggcgaggcag ttgacgccga ggcggtcgac gcggctcggc
     7621 tccaaggcac ccgtgacaag ctcaagcaaa tgctccgcga cggcaagatg gacgaccgct
     7681 tcgtcgagat cgagatgtcg cagtcggtga tgccgatgat cgaagtgctg acgccacaag
     7741 ggatggaagg catggagttc aatctcaaag acatgttttc caacatgatg cctaagaaga
     7801 ccaagaagaa gaccgtcaaa atccccgagg cactggaaat tctcacccag gaagaagcgg
     7861 ggaagctggt cgacatggac aaagtcaccg gcgaggctac tcgacgtgtc gagcagtcgg
     7921 gcatcgtctt tctcgacgaa atcgacaaaa tcgccgggcg cgagtccgcg cacgggccgg
     7981 acgtttcgcg ccagggggtg cagcgcgatc tcttgccgat cgtcgaaggc tgcaccgtca
     8041 ataccaagta cggcatagtg cgcaccgatc atattctatt tgtcgcttcc ggggcgtttc
     8101 atacggccaa gccgtcggac ttgatccccg agtttcaggg gcgctttccg attcgggttg
     8161 agctgagttc t
//
LOCUS       DASQKS010000191         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4720058, whole genome shotgun sequence.
ACCESSION   DASQKS010000191 DASQKS010000000
VERSION     DASQKS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4720058"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..497)
                     /locus_tag="VE170_04985"
     CDS             complement(282..497)
                     /locus_tag="VE170_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14821.1"
                     /translation="MGNTLRIIFFIGMVAALPGCGDKQDQAQRDEAAKQAIQQGAQKE
                     QKMMEGMVKGVETVEKKSSEAKEESKK"
     gene            657..1442
                     /locus_tag="VE170_04990"
     CDS             657..1442
                     /locus_tag="VE170_04990"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HYJ14822.1"
                     /translation="MANCADAGSTAGRFKVSGTLSATDLGGWRTIQKGVEQRKIALIR
                     SEPSSTLELKLLRFDPKFITARVLSSANWQVTAGTAKSFVEKSGAIAAINASYFDERG
                     RPLAYLKSAEREINRAVSKHALYTGIFAVADGSPTVMHRDDFSPAQASEALQSGPLLL
                     NRGAPVEIVRGLGRYARRAVLGIDKAGRVVIAVTDAVLEGLSFAELQELFTNTKWQLD
                     TPELLNLDGGGSAQLYFKSGRLEESVPGTSEVPVAIGFFVKAS"
     gene            complement(1570..3018)
                     /locus_tag="VE170_04995"
     CDS             complement(1570..3018)
                     /locus_tag="VE170_04995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYJ14823.1"
                     /translation="MTDVNLLPLLPALQVLIAALVVTVRDLFIKDGEPRGVLAFYSLI
                     GLALAGAEVLMLVGGRQESAFNETFMLDHFALFFAMIFIIAAALTILTGIHYVRQAGI
                     REGEFYALVLFATVGMIVMAAANDLMVFFLGLETMSIAVYVLTGMWRANGRSSEASMK
                     YFIMGAFATGFLLYGIALIYGATGSTNLSQISAYLLDQPTEWPVYLVGGGFLLLIGFA
                     FKVGAVPFHFWVPDVYDGAPTPVTGFMSVAVKAAAFAAWARILLYRLSPLDTDWVFPL
                     WIIAIGTMTLGNLLAITQPSVKRMLAYSSIAHAGYLLIPIVAGVEWGGQPLLFYLLTY
                     SFMTMGAFAVLSTMAEGDEAREGYSDFAGLGFKRPFLALAMSLFMLSLAGFPPLAGFT
                     GKFYVFRSAVLSGHLTLAIIGVLNSLLSVVFYLRVIVAMYMEEGGAEGKSFRQAPFVY
                     LAIAVALIGTIYLGILPADALNWSRQAFSSLE"
     gene            complement(3005..>3394)
                     /locus_tag="VE170_05000"
     CDS             complement(3005..>3394)
                     /locus_tag="VE170_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HYJ14824.1"
                     /translation="SSIGLPGLNGFVGEFLILLGSFSVTPRWTAVAATGVILGAVYML
                     WMFRRVIFGPLNNPENQKLQDLNCRELVILAPIIFLILYMGVYPQPFLSRMQPAVEVN
                     LKRIFAMPATPLAGAPSVSAERKNDGR"
BASE COUNT          799 a          993 c          953 g          649 t
ORIGIN      
        1 ggggagaaat ccttcttaga tccctcgcat tcgctcggga tgacaggctt ctggcctgtc
       61 actttgcgac cttggcgtct tttgcgcgag acaatccgag attaaccggt gcgcgaagct
      121 cacccttcga accctttgtg cccttcgtgc cttcgtggtg aacaaaacta ttgactatac
      181 gcacgcatca aaagcagcgg gccgatgcct cagaacacac cggcgagcag cgcataggcg
      241 atgccggttt ccagttccga ctgacctcaa acgaagtgat tctacttttt cgactcttcc
      301 ttcgcctcgc tcgacttctt ttcgacggtc tcaactccct tgaccatgcc ttccatcatc
      361 ttctgttctt tctgcgcgcc ttgttggatc gcctgcttgg ccgcttcgtc gcgctgcgct
      421 tgatcctgtt tgtcgccgca gccgggtaaa gccgcgacca tgccgatgaa aaaaataatt
      481 cgcagagtgt tgcccatgat tgcctccaaa attgatttgc cgcctcgtcc agttagcagg
      541 aagccgttca cagaagcaac tgcggcatga ctgttctttt ggtctcggtt tgttatgctt
      601 tcacggacaa tgacgcggtt cctcttccca tctgcgctga ttgtcttggc gctgttttgg
      661 caaattgcgc cgatgcgggc tcaaccgcgg ggcgattcaa ggtcagtggc actctcagcg
      721 ccaccgatct gggtggctgg cgaaccattc agaaaggcgt cgagcagcgc aagatcgcct
      781 tgatccgctc cgaaccgagt tccaccctcg aactgaagct gctgcgcttc gatccaaagt
      841 tcattaccgc gcgggtgtta tcgagcgcta actggcaagt gactgccggc acagcaaaga
      901 gctttgtcga gaagagcggc gccatcgcgg cgatcaatgc cagctatttc gacgagcgcg
      961 gccggcctct ggcctatctc aagtccgcag agagagagat caaccgggcg gtgtccaaac
     1021 acgcactcta caccggcatt ttcgcggtcg ctgacggcag tccgacggtc atgcaccgtg
     1081 acgatttctc gccggcccag gcgagcgagg cactccagtc cgggccgctg ttgttgaacc
     1141 gcggcgcgcc ggtggagatt gtccgcgggc tcggccgcta cgcccgccga gccgtgctcg
     1201 gcatcgacaa agccggccgc gtcgtcatcg cggtcaccga cgccgtgctc gagggcttga
     1261 gcttcgccga actccaagag ttatttacca acaccaaatg gcaacttgac accccggagt
     1321 tattgaatct cgacggcggc ggttcggccc agctctattt caaatcaggc agattggaag
     1381 aatcggtgcc cggcacctcc gaagtaccgg tggccatcgg cttcttcgtc aaagcgagct
     1441 gataaatttt gatacacaga gaaggttttc accacgaatg aggacacgaa gggttattgt
     1501 aggggcgaaa gatttttcgc ccctacggta atcgcgcaaa accttcgtgt ccttagtggc
     1561 gaatgaattc tattctaacg agctgaatgc ctggcggctc caattgaggg catcggcagg
     1621 taaaatgccc aggtaaatgg tgccgataag agcgacggca atggcgaggt aaacgaacgg
     1681 cgcttggcgg aaagattttc cctcagcgcc gccctcttcc atgtacattg cgacaatcac
     1741 gcgcaagtaa aaaaccaccg atagcaggct attgagtacg ccgatgatgg cgagagtcaa
     1801 atggccggat aaaaccgcgg aacggaaaac gtagaactta ccggtgaaac cggcgagggg
     1861 cgggaatccc gccagcgaca acatgaacag cgacatggcc agcgccaaga acggccgctt
     1921 gaatcccagg ccggcaaagt cgctgtaacc ctcgcgagcc tcatcgcctt ccgccatggt
     1981 gctgagcacg gcgaacgcgc ccatcgtcat aaatgaataa gtaagcagat agaacagcag
     2041 cggctgcccg ccccactcga cgccggcgac aatcggaatc agcagatagc ccgcatgggc
     2101 gatgctggaa taagccagca tgcgtttgac gctgggttgg gtgatcgcga gcagattacc
     2161 cagggtcatg gtgccgatgg cgatgatcca gagaggaaaa acccaatcag tgtccaatgg
     2221 cgagagccgg taaagcagaa tgcgcgccca ggcggcaaaa gcggcggctt tgaccgcaac
     2281 cgacatgaaa ccggtcactg gagtgggcgc gccgtcgtag acatccggca cccagaaatg
     2341 aaacggcacc gcgccgactt tgaaggcaaa gccgatcaac agcaagaacc cgccgccgac
     2401 cagatagacc ggccactcgg tgggctggtc aagcaggtaa gcggagattt ggcttaaatt
     2461 agtcgaaccc gtggcgccgt aaatcaaggc gatgccatag agtaaaaacc cggtggcgaa
     2521 cgcgcccatg atgaagtatt tcatcgacgc ttcgctcgag cggccgttgg cgcgccacat
     2581 gccggtgagc acgtagacag cgatcgacat ggtctccaag ccaagaaaga aaaccatcaa
     2641 atcgttggcc gccgccatga cgatcatgcc cacggtagcg aacaggacca aagcgtaaaa
     2701 ttcaccctcg cggatacccg cctggcgcac atagtggatc ccggtgagaa tggtcagggc
     2761 cgccgcgatg ataaatatca tcgcgaaaaa caacgcgaag tggtcgagca tgaaggtttc
     2821 gttgaaagcg ctttcctgcc ggccgccgac cagcatcagg acctccgccc cggccagcgc
     2881 caaaccgatc aaactataga acgccaagac cccgcgcggc tcgccgtcct tgatgaataa
     2941 atcgcgcacg gtgaccacca acgcggcgat cagcacttgc agcgccggga gcagcgggag
     3001 aagattaacg tccgtcattc ttgcgctccg cgctgacact gggtgcgccg gccaacggtg
     3061 tggccggcat agcgaaaatt cgtttcaaat tgacttccac cgccggctgc atgcggctca
     3121 agaacggctg cggataaacg cccatgtaga gaatcaagaa aatgatcggc gctagtatga
     3181 ccaattcccg gcaatttaaa tcttgcagtt tttgattctc ggggttgttg agcggtccga
     3241 agatcacccg gcgaaacatc cagagcatat agaccgcgcc aagaatcacg ccggtggcgg
     3301 ccaccgcggt ccagcgcggt gtcacgctaa acgacccgag caagatcaaa aactcgccga
     3361 caaaaccgtt gagtccgggt aacccgatgg agga
//
LOCUS       DASQKS010000192         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1358298, whole genome shotgun sequence.
ACCESSION   DASQKS010000192 DASQKS010000000
VERSION     DASQKS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358298"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VE170_05005"
     CDS             <1..849
                     /locus_tag="VE170_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14825.1"
                     /translation="KYLADVVSTGANATYQQLYPAKVLDPIKAALILPEITDPSKWYE
                     GKHHYADAEGQYVFNYVGSATYGSVNYNTKVVEPKDFKSYWDLLSPKWKDRIVARDVR
                     APGPGGGNARFFYYHPELGAPFIRKLYGEMNLTLFRDFRQGPDWLAAGKFAICFSCDV
                     EIAKQQGLPVDTFGPRVFKEGGGLVQAFGTLALVNRAPHPNAAKIFINWLLSRRGQIA
                     LQKRLSTGESPGDSLRIDIPKDDVPLLNRRLDGVKYVDTGRPEMLEMKPILDVVNEAL
                     KAAGKY"
     gene            975..2090
                     /locus_tag="VE170_05010"
     CDS             975..2090
                     /locus_tag="VE170_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14826.1"
                     /translation="MGRLILWICLGLLLPINSALAANAKPAWQLEWEKTLDGAKKEGQ
                     VVVYISGYEAILPDFEKEFPEIKVTAVTGRGNQLGPRLLAERRAEKFIADISSTGANP
                     NYQQFHVAKALDPIKPALILPEVTDQSKWYLKKHQYSDPDGQYVFNYVGSATYGSISY
                     HTKLVDVKEFKSYGDLLQPKWKGKIAIRDIREAGPGAGNTRFFYYHSELGPSFIRKLF
                     GEMDATLFRDFRQGPDWLATGKYAICFFCDVDVLKRQGLPVDTFGPKVFKEGGGLVQQ
                     FGTVSLVNRAPHPNAAKVFINWLLSRSGQIAVQRRTMTAESPADSLRIDIPKEDVPYD
                     NRRLEGIKYLDTGKPEWIEMKPIIDVVNEALKAAGKS"
     gene            2218..3357
                     /locus_tag="VE170_05015"
     CDS             2218..3357
                     /locus_tag="VE170_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14827.1"
                     /translation="MTNHLKWLTLLFACLLGLTADATAAETVKSTDEWARLVEAAKKE
                     GQLIIYGSDEYEKLFVEFHKKYPEIKVTGVYGRGADVAKRVMAERRADKFLVDLYLNG
                     LTTGYNVFYKAKVLDPIPPVLVLPEVTDLSKWWQKKHHYVDPEGKYLLNFNGGGRIVM
                     GYNTKLVAPGEIKSYWDILNPKWKGKVVGYDPSLGGSGDAMRFFYHSKSLGREFIRRV
                     LTETDIVISSDTRQMGDWLAGGKFALSIFAPVARMDLDVMQTQGIPVDWFDPEQLKEG
                     AYLTAGSGGTALLNRAPHANAAKLALNWLLSREGQTHYQRIFTTGEEGPDSLRIDIPK
                     DKVPRGNRRPVNADEARYPFVDRAEWMDQEPIRKFVKEILEQRRK"
     gene            3464..4585
                     /locus_tag="VE170_05020"
     CDS             3464..4585
                     /locus_tag="VE170_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14828.1"
                     /translation="MMIQWQKLIGLVGIFVLALAAEVGAAQTDWKTDWELTVNAAKKE
                     GQVNVYIYRYERLLEDFKREFPGINVVAVTGRGNELTNRVMAERRAGKFIADVYSAGT
                     GGNYNVLYKGKALDPIKPLLSLPEVVDESKWYGKEHRYADAEGKYVFAYLASPSDAQL
                     AFNTSQVSPKEFKSYWDVTQPKWKGKIVSLDPRDGGLGGTMQFFYYHPELGVEFIKKF
                     FTTMDIQFAKNFRQMTDWLAQGRYALCFGCKDSMRAKNQGLPVDDFDTTRWKEGSSFS
                     SGGGSLSYLNQAPHPNAAKVFINWFLSRRGQIALQKLGDVDDAPNSRRIDIPKDDVLP
                     GNRMSPEGRYFDVVKPEYADLKPIADLAREIMAAIEAKK"
     gene            4726..>5040
                     /locus_tag="VE170_05025"
     CDS             4726..>5040
                     /locus_tag="VE170_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14829.1"
                     /translation="MIRRQVGKIVLLGLVLFSPASPTQAAQSDWKKDWEQTVAAAKKE
                     GQVTVYIYRYEGLLQDFKRDYPGINVVAVTGRGNDLTNRIMAERRGGKFIADIYSGGT
                     NSL"
BASE COUNT         1163 a         1320 c         1492 g         1065 t
ORIGIN      
        1 aagtatctcg ccgatgtggt cagcacggga gccaatgcga cctaccagca gctctatcca
       61 gccaaagtat tggacccgat caaagcggcg ctgattctgc cggagattac cgacccgtcc
      121 aaatggtatg agggaaagca ccattacgcc gacgccgagg gccaatatgt tttcaattac
      181 gtcggctcgg cgacctatgg ctccgtcaac tacaacacca aggtggtcga accgaaggat
      241 tttaaatcct actgggatct gctcagtccg aaatggaagg acagaatcgt cgcccgcgat
      301 gttcgggccc ccggccccgg cggcggtaac gcccgcttct tttattatca tcccgagttg
      361 ggcgcgccgt ttattcgaaa actctacggc gaaatgaatc tgactctgtt ccgtgatttt
      421 cgccaggggc cggactggct ggcggcggga aagttcgcca tctgtttttc ctgcgacgtc
      481 gagatagcga aacagcaagg cttgccggtg gacacctttg ggccgagggt tttcaaagaa
      541 ggcggcggct tggtccaagc cttcgggact ctggcgctgg tcaatcgcgc gccgcatccc
      601 aacgcggcga aaatatttat caactggcta ttgtcgcgcc gcggccagat cgcgctgcaa
      661 aagcgtttga gcaccggcga aagccccggc gattcgctgc gcatcgacat tcccaaagac
      721 gatgtgccgc tgctgaatcg gcggctcgac ggtgtcaagt atgtcgatac cggaagaccc
      781 gagatgctcg agatgaaacc gattctcgat gtcgtaaacg aagcgctaaa ggcggctggg
      841 aaatattaat cctaaatctt ggcgtccctt cgggatggct cagggcgtgc ttggcatctg
      901 agccgaggaa aataccgaac cggatttaca ttggaatgat ctcaagatgc aaagtcgaga
      961 aagcggaggc tagtttggga aggttaattc tgtggatatg cctggggctg ttgctaccga
     1021 tcaactctgc gttggcagcc aatgccaaac ctgcttggca gctggaatgg gaaaagactc
     1081 tcgacggggc gaaaaaagaa gggcaagtgg tcgtttacat cagcggctac gaggcgatcc
     1141 tgccggactt cgagaaggag tttccagaga tcaaagtcac tgcggtcacc gggcgcggca
     1201 atcagcttgg gccgcgcttg ttggcggaac gccgcgcgga aaagtttatc gctgatattt
     1261 cgagcaccgg ggcgaacccc aattaccaac agtttcacgt cgccaaggcg ctcgatccga
     1321 tcaagcccgc actaatattg ccggaagtca ccgatcaatc gaagtggtat ctgaagaagc
     1381 atcagtattc cgatcccgac ggccagtatg ttttcaacta tgtcggctcg gcgacctacg
     1441 gctcgatcag ctatcacacc aagctcgtcg acgtgaagga gttcaagtcc tacggcgatc
     1501 ttttgcaacc gaagtggaaa ggcaagatcg cgatacgcga tatccgcgag gctgggccag
     1561 gcgccggtaa tacgcgcttc ttttactatc actccgaact tgggccatcg ttcattcgta
     1621 aactgtttgg cgagatggat gcgactttgt ttcgtgattt ccgccagggc cccgactggc
     1681 tggccacggg gaaatatgcg atctgctttt tttgcgacgt cgatgtcttg aagcggcagg
     1741 ggctgccggt ggacactttc ggtccgaaag tcttcaaaga aggcggcggg ctggtgcagc
     1801 agttcggcac cgtctctctg gtgaaccgcg cgccccatcc caacgccgcc aaggttttca
     1861 tcaactggct gctgtcgcgc agcggccaga tcgccgtgca aagaagaact atgactgcgg
     1921 aaagtccggc agattctttg cgcatcgata ttcccaagga agatgtgccg tacgacaatc
     1981 gccggttgga gggaatcaaa tatctcgaca ccggcaagcc ggagtggatc gagatgaagc
     2041 cgatcatcga cgtggtaaac gaagcgctga aggcggcggg gaagagttag ccagccgtcg
     2101 ttgtcctatt gtggtggagc ctgtgaccgc ccaggcaacc aaggggactg ctccgacaaa
     2161 agcaataacg ctgagaacag tttcgaattt ttattttgca ttgggagatt agcaagcatg
     2221 acaaaccatc taaaatggct gacgctgctg ttcgcctgct tacttgggtt gacagcagat
     2281 gccaccgccg cggagacagt gaagtcgacg gacgaatggg cgcggctggt cgaggcggcc
     2341 aagaaggaag ggcagctgat tatttacggc agcgatgagt acgagaagct cttcgttgag
     2401 tttcacaaga agtatcctga gatcaaagtg accggggtat atggccgcgg cgctgacgtt
     2461 gccaagcggg tgatggcgga gcggcgcgcg gataaatttc tcgtcgatct ttatctcaac
     2521 ggcctcacca ccgggtacaa tgttttctac aaagccaaag tgctcgaccc gatcccgccg
     2581 gtattggttt tgccggaagt caccgacctg tcgaagtggt ggcaaaaaaa gcatcactac
     2641 gtcgatcccg aaggcaagta tttgctaaac ttcaacggcg gcggtcgcat cgtcatgggt
     2701 tataacacca agctcgtcgc tcccggcgag atcaaatcct actgggatat cctcaatcct
     2761 aaatggaaag gcaaagtcgt cggctacgac cccagtctgg gcggctccgg cgacgccatg
     2821 cggttttttt atcacagcaa gagtttgggc agggagttta ttcgccgggt gctgaccgaa
     2881 accgacatcg tcatcagcag cgacacgcgc cagatgggcg attggttggc cggcgggaaa
     2941 tttgcgttat cgatctttgc gccggtggcg cgcatggatc tcgacgtcat gcagacccag
     3001 gggattccgg tggactggtt cgaccccgag cagttgaaag aaggcgcgta tctcaccgca
     3061 ggatccggcg gcacggcgtt gctcaacaga gcgccccacg ccaatgcggc gaagttggcg
     3121 ctcaattggc tgctctcgcg cgaagggcag acacactatc agcggatctt caccaccggc
     3181 gaagaaggac cggattcgct gcgcatcgac atccccaaag acaaagtgcc gcgcggcaat
     3241 cgccgcccgg tcaacgcgga tgaagcccgc tatcctttcg tcgaccgcgc cgaatggatg
     3301 gatcaggaac cgatccgtaa gttcgtcaag gaaatattgg aacagcgacg aaagtgatct
     3361 tggatcaccg gaaatatcat ttaagaaaaa accatgagta aactaatcgc ctcgttttgg
     3421 ccttcatgtt cttggttcta agcaggaggc ttcggtgatc acgatgatga ttcaatggca
     3481 aaaactgatt ggtctggttg ggatttttgt cttggcgttg gcggcggaag ttggcgccgc
     3541 gcaaacggat tggaaaaccg attgggaact gaccgtcaac gccgcgaaga aggaagggca
     3601 ggtcaatgtt tatatctatc gctatgagcg cttgctggag gatttcaaac gggagtttcc
     3661 cggcatcaac gtggtcgctg tgaccggacg gggtaatgag ctgactaacc gggtcatggc
     3721 tgagcggcgg gcaggcaagt tcatcgcgga tgtctatagc gccggaaccg gcggcaacta
     3781 caacgttctt tacaaaggga aggcgcttga cccgatcaaa ccgctgttga gcctgcccga
     3841 agtcgtcgat gagtcgaaat ggtatggcaa ggagcaccgc tacgccgacg ccgaggggaa
     3901 atatgttttc gcctatctcg ccagtccgag cgacgctcag ttagctttta acacgagcca
     3961 ggtgagtcct aaggaattca aatcctattg ggatgtcacg cagcccaagt ggaagggtaa
     4021 aatcgtctcc ctcgatccgc gcgacggcgg cttgggcggc accatgcagt ttttctacta
     4081 tcacccggaa ctgggcgtcg aatttatcaa aaaattcttt accaccatgg acatccagtt
     4141 cgccaagaat ttccgccaga tgaccgactg gctggcccag gggcgctacg ccctgtgctt
     4201 tggctgcaag gattccatgc gcgctaaaaa ccagggactg ccagtggatg atttcgatac
     4261 cacccggtgg aaggagggat cgagtttttc gtcaggcggc ggttcgctca gttatttgaa
     4321 tcaggcgccg caccccaacg cggccaaggt ttttatcaac tggttcctgt cgcggcgtgg
     4381 gcagatcgcc ttgcaaaagc tcggcgacgt cgacgatgcg cccaattcgc ggcgcatcga
     4441 tatccccaag gatgatgttc tgccaggcaa tcgcatgtca ccggagggca ggtatttcga
     4501 tgtggtcaaa cccgagtatg ccgacttgaa gccgatcgcc gatctcgcca gggagataat
     4561 ggcggccatc gaagcgaaga agtagaccga tagattgcca agttgttgcc gtgtcgtgcg
     4621 tgcatggata gcgattggct ggttgacatt ggcttgccca tcgggaaata ttcggccggt
     4681 gcgcgcggtt gcggcaaatc ttcagcggag cggagaaaga tgtgcatgat taggcgtcaa
     4741 gtcggcaaaa ttgttttgct cgggttggta cttttctctc cagcgtcgcc tacgcaggcg
     4801 gcccaatccg actggaaaaa ggactgggag cagacggtcg cggcggccaa gaaggagggc
     4861 caggtcaccg tctatattta ccgctacgag ggtttgctgc aggatttcaa gcgcgattat
     4921 cccggtatca atgtcgtcgc ggtcaccggc cggggcaacg atctgaccaa ccgcatcatg
     4981 gccgagcggc gcggcggcaa gtttatcgcc gatatttaca gcggcggcac caacagtctt
//
LOCUS       DASQKS010000193         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5137007, whole genome shotgun sequence.
ACCESSION   DASQKS010000193 DASQKS010000000
VERSION     DASQKS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5137007"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1037)
                     /locus_tag="VE170_05030"
     CDS             complement(60..1037)
                     /locus_tag="VE170_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14830.1"
                     /translation="MKRIMFAIAVAAMCLSEVHGADRIRIGYSSISGAYTPIWVAHDA
                     GFFAKEGLQDDIILIPSGTQLAQVTVAGEIDIGSLNGSSAIAAALSGADLKIIGNSGN
                     KMVFSLYVRPEIKNVDGLRGKKIGITRFGSAPDISVRYALRKHNINPDKDLTLIQLGF
                     MATVAAGLQGGSIEGGVVSPPTQFAIDKAGFKELISITDMDYAFPNPALVAVGSIIRS
                     KPDVINRFMRAYTRGVHKARTDREFTYKSLAKYTKINDQVVLQKAYDFYMTKVLEKTP
                     AVNMAGVVNVLEDLVKSVPAAKNAKPEQFVDMRFLDTLEKSGLLKELYP"
     gene            complement(1531..2157)
                     /locus_tag="VE170_05035"
     CDS             complement(1531..2157)
                     /locus_tag="VE170_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ14831.1"
                     /translation="MILPLNVVLFSVGSRGDVSPFIEIGRRLKARGHTVNLISHFSYK
                     ETAERAGLDFVAVDSPREYQEFIADQHLLNDPRGVAEFIRRHSLSKIAEHSELIAQSC
                     EPRNTVLVTRDLFDTAIRLTAERSRLPVTWIFGFPSQATTWKLRQQLFSEVLRPDINR
                     ARAALGLQKVVAGDARLEYPSGAIGLWPEWFAEVEFDLPFKVTPVGFF"
BASE COUNT          512 a          629 c          607 g          527 t
ORIGIN      
        1 cgtgaagaaa tccgcagtcg acttacccct taccgttcac ccctcacgtc ttctgcggtc
       61 tacggataaa gttctttcaa caagccgctc ttctccagag tatcgagaaa gcgcatgtcg
      121 acgaattgct caggcttggc gtttttcgcg gcgggcacgg acttgaccag atcttcgagg
      181 acgttgacga cacctgccat gttcaccgcc ggtgtcttct ctaaaacttt ggtcatatag
      241 aaatcgtagg ctttctgcag caccacttga tcgttgatct tggtgtattt ggccagcgac
      301 ttataggtga actcgcggtc ggtgcgcgcc ttatgcacgc cacgagtgta agcgcgcatg
      361 aaccggttga tcacgtcggg tttgctgcga atgatcgagc ccaccgccac cagcgccgga
      421 ttggggaaag cgtaatccat gtcggtgatg ctgatcaact ctttgaagcc ggctttgtcg
      481 atggcgaatt gagtcggcgg cgacactacg ccgccctcga tggagccgcc ttgtagtcca
      541 gccgccaccg tcgccatgaa gcccaactgg atcaacgtca ggtctttatc gggattgatg
      601 ttgtgtttac gcagcgcgta gcgcaccgaa atatccggcg ccgagccaaa gcgtgtgatg
      661 ccaattttct tacccctgag cccgtcgaca tttttaatct ccggccggac gtaaagtgaa
      721 aaaaccatct tgttgcctga gttgccgatg attttcagat cggcgccgga caacgccgcg
      781 gcgatcgccg agctgccatt gagcgagccg atgtcgattt cacccgctac cgtcacttgc
      841 gccagctgcg tgccgctggg aatcaaaatg atatcgtctt gcaacccttc cttggcaaag
      901 aacccggcat catgggcaac ccagatcggc gtataggcgc cgctgatcga gctgtagccg
      961 atgcgaatac ggtcggcccc atggacctcg ctaaggcaca tcgcagcgac ggcaattgca
     1021 aacataattc gcttcattgg ttatcctttc aggaagtttc gcagtgactt cgagcggtaa
     1081 tacttcattt gtcgaccatt ttttcaaagc tcacggagcc aatctagcag ccaagattga
     1141 cgataaacgg gcattaacga aagcgtctac gcgaaaggag catactctaa tccggggcaa
     1201 tgataaatca cggcgagttt tgctgaaaat agcgatacga atcaggcgcg tgcatgttgt
     1261 tcgataagct cacacgccga ggcggcgccg tcggtgcttt ccaatcgggc ggccaacttc
     1321 cggcagtttg cgccaacctc tggcgaacgg agcaaccgaa gcatggcgtc cgcgataatc
     1381 gccggctgcc attttggcgg gggtaagaat tccgcgatcc gcagctgctt caaatgggac
     1441 gcgttgtccg gacgatccgc ccctttggga aggaccagtt gggggactcc ggcagccatc
     1501 gcgcatccag ggttcatggc caccgtcata tcaaaaaaac cctaccgggg tcactttaaa
     1561 cggcaggtcg aattcaacct cggcaaacca ttctggccac aatccaattg cacccgatgg
     1621 atactcaagc cgcgcatctc ctgccacgac cttctgcagg cccagggctg cccgcgctcg
     1681 attgatgtcg ggtcgtaaaa cttcggagaa cagttgttgt cgaagtttcc aggttgtcgc
     1741 ttgactggga aagccgaaaa tccaggtcac aggtaagcga ctcctttcgg cagtcaagcg
     1801 tatcgcggta tcgaagagat cgcgggttac gagaacggtg tttcgtggct cgcacgattg
     1861 tgcgatcaac tcggaatgtt ccgcaatctt ggataaagaa tgtcgtcgta tgaattctgc
     1921 tactccccta ggatcgttca agagatgctg atcggcgatg aattcttgat attctcgggg
     1981 actgtcgacg gcgacgaaat ctaacccggc acgttcagct gtttctttgt aactaaaatg
     2041 gcttatgaga ttgaccgtgt gaccgcgcgc tttgagccgc cgtccaattt caatgaacgg
     2101 cgacacatca ccccgggatc cgacagaaaa aagaaccaca tttaacggca gtatcactta
     2161 ggtcctgcgg cgcggtccta cttgcgctcc agcagttgcg tctcagataa cttgccgggc
     2221 acgaggttct cggcttgctc atcggtaagt tccgacagtt tctgaaaaat ctggt
//
LOCUS       DASQKS010000194         7837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1358625, whole genome shotgun sequence.
ACCESSION   DASQKS010000194 DASQKS010000000
VERSION     DASQKS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358625"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VE170_05040"
     CDS             complement(<1..211)
                     /locus_tag="VE170_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYJ14832.1"
                     /translation="MADPVLLLTIFIIAVALAFDYVNGFHDAANSIATVVSTRVLTPM
                     QGVVWAAFWNFIAAFTFGTAVAKTIG"
     gene            complement(204..833)
                     /locus_tag="VE170_05045"
     CDS             complement(204..833)
                     /locus_tag="VE170_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HYJ14833.1"
                     /translation="MAKFQFLPAETKFYDWFEKATANLLEGAAALQDMFDHYENVEAK
                     VAHITELEHRGDFIVHEVMHLLMHTLIIPLDSEDIERLISALDDAIDGIEAAAVRMVI
                     FQVEAPTERARQLAQLIHRGAQELHAAMPNLREKKTFRLVTEHIKEINTIENNGDRVL
                     REALVDIVNRKEDLFNLIRWKEIYEMLEEATDRMEDIADVLQRVVIKNG"
     gene            complement(941..3157)
                     /locus_tag="VE170_05050"
     CDS             complement(941..3157)
                     /locus_tag="VE170_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ14834.1"
                     /translation="MKRLWNFSLVKKLFFSYFAVALLLVLGFYVFSITLIRDFHITTL
                     SSRLEQEAHLLGRVLPFGIEGDELDAACRQLAGELGSRITVIALDGKVLGDSAEPSAK
                     MENHFSRPEVIEALKSVTGSAERYSTTVGFDMLYRAFHQRNDKQQRIVRIAIPLKELD
                     IVIRGMRRSLITGLILASVAGLLLAWWFTNYLRERMQRLVQFSGKIAQGIYPQAFFSG
                     HDRDEIGLLERQLNNMSQRIRDNLAEIAGEKDKADSILRCMIEGVLVLDPKGNVLVIN
                     DQAKTMFQVPAGREVHGASVLEISRHPDIRSILDEAVRFDFTSAPYSKEIELEGGRWF
                     SVNAAPLRNAQRMTLGSILVFHDVTEVKRLETIRSDFVANVSHELRTPLTAIQGYVET
                     LIHSPPADSRERQQFLEIIERHAERLGRLTEDLLSLADLESGKIQIAPRPVDSGKLIQ
                     QVLEIFWERAAKKEIRLNHEISAAYGKINGDPDRLQQLLINLVDNAVKFTPSGGTVTI
                     RAAECQADNGARRIEISIADSGVGIPEKDLPRLTERFYRVDKARSRDLGGTGLGLAIV
                     KHIVQAHGGELKITSELNKGTTISVRLPIVENGAASNDGILFLCTGNSCRSQMAEGFA
                     RRLVHNGQKVYSAGTSPKGVHPLAVRVMQEVGIDISSQESKGLDKIPLRQIGHLITLC
                     DDAAESCATLPGGFKRTHWPSSDPVAAHGTEAEVLPHFRLARDQIRERIAALYSKP"
     gene            complement(3135..3854)
                     /locus_tag="VE170_05055"
     CDS             complement(3135..3854)
                     /locus_tag="VE170_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ14835.1"
                     /translation="MGNGAYKILVVEDEPDIRKLINYNLTQDHYRVVEAESGDQALKL
                     IQRDKPSLVILDLMMPGMSGLEVCRALRSHDETAKLPILMLTAKAGEADRVLGLELGA
                     DDYLAKPFSPRELVARVRAILRRVNGAGQTETLPAYQKGGLKIDFATYEVAVRGKAIK
                     LTLKEFELLKFLVQNPSRVLNRDQLLDRVWGGETFVTPRTVDVHIRRLRKAIEKDDSN
                     PKWILTLRGVGYKFDEKALEF"
     gene            complement(3861..4547)
                     /gene="phoU"
                     /locus_tag="VE170_05060"
     CDS             complement(3861..4547)
                     /gene="phoU"
                     /locus_tag="VE170_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HYJ14836.1"
                     /translation="MTTKHTDKKYEEELTKLREEILYMGGLIEDQIQKAIKSLVDRDS
                     DLAKIIIERDHEVNRLDVEIDELCIRLLALHQPAGIDLRLITTGLKITTDLERIGDMA
                     VNICERALELNQEAQLKPYIDIPRMARISQRMIHESLDAFVREDTDLALKVCKDDANV
                     DQLNAQIFRETITFMLEDAQTISRATKISSISKYLERIADHATNIAEMVIFMVKGKSI
                     RHMKELPPSV"
     gene            complement(4562..5365)
                     /gene="pstB"
                     /locus_tag="VE170_05065"
     CDS             complement(4562..5365)
                     /gene="pstB"
                     /locus_tag="VE170_05065"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HYJ14837.1"
                     /translation="MKAIDSSVIRASSEVAHKNIFTVENVDFFYGEKRALKSINLAIG
                     EKKSTAFIGPSGCGKTTLLRCLNRMNDLVLGTRVTGQIKLEGKNIYDPDLDVINLRRR
                     VGMIFQKWNPFPKSIYENIIYGLRIVGINNRAYLDEVVEKSLHRAALWDEVKDNLHKS
                     AADLSGGQQQRLCIARALAVEPEVLLMDEPCSALDPISTAKIEELIHELKDTFTIVIV
                     THNMQQAARVSDYTAFFYLGELIEFGETGKLFTAPEKRQTEDYITGRFG"
     gene            complement(5458..6381)
                     /gene="pstA"
                     /locus_tag="VE170_05070"
     CDS             complement(5458..6381)
                     /gene="pstA"
                     /locus_tag="VE170_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HYJ14838.1"
                     /translation="MEQPSASAIRKSVAGNKTRDKVFAIVGLISTFIGMLTLAALLFD
                     LSVDGLSRMKPHFFTSFPSRFAGQAGILSSWVGTILVMIVTALAAVPIGIGAGVYLEE
                     YAPKNWVTALIEINIANLAGVPSIVYGLMALGLFVYQFKFGQSILSAGLTLALLILPI
                     IIVATRESLRAVPGSMREAAYALGATKWQTTSDHVLPYSMGGILTGVIIGLSRAIGET
                     APLITIGALTFIAFLPDAPVKAEFPFISFDWLWSPYSVMPIQMFNWVSRPQAEFHLNA
                     AATGLVLMVMTLAMNAVAIYFRYRFRKRIKW"
     gene            complement(6384..7334)
                     /gene="pstC"
                     /locus_tag="VE170_05075"
     CDS             complement(6384..7334)
                     /gene="pstC"
                     /locus_tag="VE170_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HYJ14839.1"
                     /translation="MSKSEVAIDYTDVRERIRSHSGSVASRKIRERIIEGGMFLCAVF
                     SVAVTVGIVWVLVYESWNFFRNVSLIDFLTDTQWTVLFENPRYGILPLVTGTLVTSMI
                     ALVVALPLGTIIAIYLSEYANSTVREILKPILELLSAVPTVVFGYFALLFVTPMLQNI
                     LPELPGFNMLSAGIVIGIMIVPYVSSLSEDAMKAVPVTIREGSYAMGATRVQTALRVL
                     MPAAFSGIASAYVLGFSRAVGETMVVALAAGTQPNLTLNPMEGAATITAYIVQVSLGD
                     LPHGSIAYQSIFAAGLTLMVMTLVFNIGGYFLRKRFREVY"
     gene            complement(7402..>7837)
                     /locus_tag="VE170_05080"
     CDS             complement(7402..>7837)
                     /locus_tag="VE170_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYJ14840.1"
                     /translation="ASEDDNVLVQGVINDRNALGYFGFAYYIENQAKLKAVAIDGGKG
                     AVGPSPKTVEDGTYQPLSRPIFIYVSKKSLDSKPEVKEFIDFYLKNAAKLVKDVKYVA
                     LPASVYKSAAEHVTKGKIGTVFGGTAEVGVKIEDLMKREANF"
BASE COUNT         1771 a         2070 c         2137 g         1859 t
ORIGIN      
        1 tgccgatggt tttagccacc gcagtaccga aagtaaacgc cgcgatgaag ttccaaaacg
       61 cggcccacac tacaccttgc atcggcgtca aaacacgcgt tgaaactact gtggcgatgg
      121 agttggccgc gtcgtgaaag ccgttgacgt agtcgaaggc gagggcgacg gcgatgataa
      181 aaatcgtcag cagcaggacc ggatcagcca tttttgatga ccacccgctg cagcacgtcg
      241 gcgatgtctt ccatgcggtc ggtcgcttcc tcgagcatct cgtagatctc tttccagcga
      301 atcaagttga aaagatcttc cttgcggttg acgatgtcga ccaaagcttc ccgcagaact
      361 cggtcgccgt tgttctcgat ggtattgatt tccttgatat gttcggtgac caagcggaag
      421 gtctttttct cgcgcaggtt gggcatcgcc gcgtgcagct cttgagcgcc gcgatggatc
      481 aactgggcta gctggcgtgc ccgttcggtg ggggcttcga cctggaagat caccatgcgc
      541 acggcagcgg cttcgatgcc atcgatggcg tcgtcgaggg ccgaaatcag ccgttctata
      601 tcctcgctat cgagcggaat aatcagcgtg tgcatcaaaa ggtgcatgac ctcgtgcacg
      661 ataaagtctc cgcgatgctc taattccgtg atatgggcga ccttggcttc gacgttttcg
      721 taatggtcga acatatcctg tagcgccgcc gcgccttcta ggaggttcgc cgtagctttc
      781 tcaaaccagt cgtagaattt cgtctcagcc ggcaaaaact gaaatttcgc catgatcgct
      841 ccgagaaaac ggtttagata ttggaatata agaaccgaga atacgaaacg cgatgagatt
      901 tatcacgcgt gtcgcggagc ttcaactgga atgaaaagat ttatggcttg gaatacaggg
      961 cggcaatccg ttcgcggatc tgatcgcgag caagacggaa atggggcagg acttcggcct
     1021 ctgtgccatg tgccgcgacg gggtcggacg aaggccaatg ggtgcgtttg aatccgccgg
     1081 gtagggtggc gcagctttcg gcagcatcgt cgcacagagt gataaggtga ccgatttgcc
     1141 gcaagggaat cttgtcgagt ccctttgact cctgactgga aatatcgata ccaacttcct
     1201 gcataacccg gaccgccaaa gggtgaacgc cttttggact ggttcccgcg ctataaacct
     1261 tctgtccgtt gtgaaccaac cggcgggcaa agccttcggc catctggctg cggcaagaat
     1321 tgcctgtaca tagaaacaaa ataccatcgt tagaagcggc accgttttcg acaattggca
     1381 aacgcacgct aatcgtcgtg cccttgttca gctcgctggt gattttcagt tcgccgccat
     1441 gcgcctggac gatatgtttg acgatcgcca ggccgagacc cgtgccaccg agatcccgcg
     1501 agcgcgcctt atcgacacgg tagaagcgct ctgtcagtcg cggcaaatct ttttccggga
     1561 tacccacgcc gctgtccgcg attgagattt cgatccgccg agcgccgttg tccgcttgac
     1621 attccgctgc gcgaatcgtc accgtgccgc cagacggtgt aaattttacc gcattgtcaa
     1681 ccagattgat caacagctgt tgcaggcggt ctggatcgcc gttgattttg ccgtacgcgg
     1741 cagaaatctc atggttgagt ctgatttctt ttttggccgc cctctcccag aatatttcca
     1801 ggacctgctg tatgagtttg ccgctatcca ctggccgcgg cgcaatctgg atcttgcccg
     1861 actcgagatc ggccaggctc aacagatctt cggtcaaccg gcccagtctt tcggcgtggc
     1921 gctcaatgat ttcgagaaac tgttggcgct cccggctatc ggccggcggc gaatggatga
     1981 gggtttcgac atagccttgg atcgctgtta acggcgtccg taattcgtgc gacacgttgg
     2041 cgacaaaatc agagcgtatc gtctcgagcc gtttgacctc cgtgacatcg tgaaaaacca
     2101 agatcgagcc caaggtcatg cgttgggcgt tgcgcagagg cgccgcattg acactgaacc
     2161 aacggccacc ctcgagttcg atctctttgc tgtagggggc gctggtgaag tcgaaccgaa
     2221 cggcttcatc caagatcgac cgaatgtccg gatgacgcga aatttccagt actgatgctc
     2281 catgcacttc acgtccagct ggcacctgaa acatcgtctt ggcttggtcg ttgatgacca
     2341 ggacattgcc cttggggtcg aggaccaata ctccctcgat catgcagcgc aagatcgaat
     2401 cggctttatc tttttcgccg gcaatttcgg caaggttgtc gcgaatccgt tgactcatgt
     2461 tgttcaattg acgttcgagc aggccgatct catctcggtc gtgaccagaa aaaaatgctt
     2521 gggggtagat accctgagcg attttgccgg aaaactgtac cagccgttgc atgcgctctc
     2581 tcaaatagtt tgtaaaccac caggcgagca agagcccggc caccgaagcg agtatcaagc
     2641 cggtgattaa cgagcgccgc atgccgcgga tgacgatgtc gagttccttt aagggaatgg
     2701 cgatacggac gatgcgctgt tgtttgtcgt tgcgttgatg aaatgcccgg tagagcatgt
     2761 cgaagccgac cgtggtgctg tagcgttcgg cgctgccggt aacggatttt aaagcttcga
     2821 tcacctccgg ccgactaaaa tggttttcca ttttagccga aggctcagcg gaatcgccta
     2881 agacttttcc gtcgagggcg atcacggtga tgcgcgaacc cagttcgccg gcgagttggc
     2941 ggcaagccgc gtcaagttca tcgccttcga tgccgaacgg caaaactcgg ccaagcaagt
     3001 gcgcctcttg ctccagccgg ctgcttagcg tggttatgtg gaaatcgcga atgagggtaa
     3061 tggaaaaaac gtagaaccct aaaaccaaca gcagagctac cgcaaagtaa gaaaaaaata
     3121 gttttttaac gagactaaaa ttccagagcc ttttcatcga atttgtagcc gactccgcgc
     3181 agagtcaaaa tccatttggg attactatcg tccttttcta ttgctttgcg caagcgacga
     3241 atatgcacgt cgactgtgcg cggcgtgaca aaggtttcgc cgccccagac gcgatcgagg
     3301 agttgatcgc ggttgagcac ccggctggga ttttgcacaa gaaattttaa cagttcgaac
     3361 tctttcagcg tcaatttgat cgctttgccg cgcaccgcga cttcgtaggt ggcaaagtcg
     3421 atttttaaac cgcctttttg ataggccggc agagtttcgg tttggccggc gccattgacg
     3481 cgacgcagga tggcacgcac gcgggcgacc agttcgcgcg gactaaacgg tttagccaaa
     3541 tagtcgtctg cgcctaattc caaccctaag actcgatcgg cttcaccagc cttcgcggtc
     3601 aacatgagga tcggtagctt agcggtttcg tcgtggctac ggagcgctcg gcatacctcc
     3661 aaccctgaca ttccaggcat catcaggtcg aggatgacca gactcggctt gtcgcgctga
     3721 ataagtttaa gcgcttggtc gccgctttct gcctcgacaa ccctgtaatg gtcctgggtc
     3781 aagttgtagt ttatcaactt acggatatcg ggctcgtctt cgacgacgag aatcttgtat
     3841 gcgccatttc ccatagttaa ttaaaccgac ggcggcagtt ccttcatgtg acgaatgctt
     3901 ttacccttga ccatgaagat caccatttcg gcgatattgg tcgcatgatc ggcaatccgt
     3961 tcgaggtact ttgaaatcga gctaattttg gtcgctcggc tgatggtctg ggcgtcttcc
     4021 aacatgaatg taatggtttc gcggaagatt tgcgcattca gttgatcgac attggcgtcg
     4081 tctttacaaa ccttgagcgc caggtcggtg tcttcccgga caaaggcatc gaggctttca
     4141 tgaatcattc gctgggaaat gcgcgccatg cgcggaatat cgatataggg tttgagctgc
     4201 gcctcctggt tcagttcaag cgcgcgttcg cagatattga ccgccatgtc gccgatgcgc
     4261 tccaaatcgg tggtgatctt gaggccggtg gtgatcaagc gcaggtcgat cccggcgggt
     4321 tggtgcaacg ccaagagcct gatacacaac tcgtcgatct cgacatccaa acggttaact
     4381 tcatggtccc gttcgatgat gatcttggcg agatcggaat ctcggtcaac cagtgactta
     4441 atcgcctttt gaatctggtc ttcgatcaat ccgcccatgt aaagaatttc ttcacgcagt
     4501 ttggttagct cttcttcgta ttttttgtcg gtatgttttg tcgtcatggt tgcctccgga
     4561 atcagccgaa tcggccggtg atgtaatctt cggtctggcg cttttccggg gccgtaaaaa
     4621 gcttgccagt ctcgccaaac tctatcaact cgccgagata gaaaaaggcc gtgtagtcgg
     4681 atacgcgagc ggcttgctgc atgttatggg tgacaatgac gatggtgaac gtgtccttta
     4741 actcgtggat caattcttct atcttcgccg tcgatatcgg gtcgagggcg gaacagggtt
     4801 cgtccatcaa caagacttca ggttcgaccg ccagggcacg cgcgatgcag aggcgctgct
     4861 gttggccgcc cgaaagatca gcggcgcttt tgtgcaagtt gtctttgact tcatcccaga
     4921 gcgcggcgcg gtgtagactt ttctccacca cctcgtccaa gtaggcgcgg ttattgatgc
     4981 cgacaatgcg caggccataa atgatattct cataaatgct ttttggaaaa ggattccatt
     5041 tttgaaaaat catgccgacc cgccggcgca agttgatgac gtcgaggtcc gggtcgtaaa
     5101 tatttttgcc ttcgagcttg atttgcccgg tgacccgggt ccccaggacg aggtcattca
     5161 tgcggttcaa gcagcgcagc aaggtggtct tgccgcagcc ggaggggccg atgaatgcgg
     5221 tcgatttttt ttcaccgatc gccagattga tggatttcag cgcccgtttt tcaccgtaaa
     5281 aaaagtcaac gttttcgacg gtaaaaatat tcttgtgcgc tacctcagag ctggctcgaa
     5341 ttaccgagct gtctatagct ttcatcgctg gtcgtacgac ggcggtcgct tccttagttt
     5401 cctgctgcag gctcatctta cctccattgg ggctctttgt cattggcgtc ccggccgtta
     5461 ccacttgatg cgtttgcgga accggtagcg aaaatagatc gcgacggcgt tcatggccaa
     5521 ggtcatgacc atgagcacca accccgtcgc cgcggcattg aggtgaaact cggcttgagg
     5581 ccgtgacacc caattaaaca tttgaatagg catgaccgaa tagggtgacc agagccagtc
     5641 gaaagaaatg aacggaaact ccgccttgac cggagcatcc ggcagaaaag cgatgaaggt
     5701 cagtgcgccg atcgtaatga gcggcgccgt ttcaccgatc gcgcgagata gaccgataat
     5761 gaccccggtt aaaatacccc ccatggaata gggcaggaca tgatcactgg tggtctgcca
     5821 tttggttgcg ccgagggcgt aggcggcttc tctcatgctt cccgggacgg cgcggaggga
     5881 ctctcgggtg gcaacgataa tgatcggcaa aattagcaaa gccagcgtga gcccggcgga
     5941 caggatgctt tgtccgaatt tgaattgata aacaaacagt ccaagggcca tcaagccgta
     6001 aactatcgac ggcacgccgg cgagattggc gatgttgatc tcaattagcg cagtaaccca
     6061 gttcttcggc gcatactcct cgagatacac accggcacca atcccgatcg gtaccgccgc
     6121 cagcgccgtg acaatcatca ccagaatcgt gccgacccag gaggaaagaa taccggcttg
     6181 gcctgcgaaa cgtgacggga aagaggtgaa aaagtgaggc ttcatgcgcg agagtccatc
     6241 gaccgacagg tcgaagagca aagcggctaa cgtcagcatg ccgataaacg tggaaatcag
     6301 gccgacgatc gcaaagactt tgtcgcgcgt cttattgccg gctacgcttt tcctgatggc
     6361 tgacgcagac ggttgttcca tgactagtaa acctctctaa agcgcttacg caggaagtaa
     6421 ccgccgatat tgaataccaa agtcatcacc atcaacgtca gaccggcggc aaagatactt
     6481 tgataggcga tactgccgtg cggcaaatcg cccaagctca cttggacgat gtaggcagtg
     6541 atggttgccg cgccttccat tggattgagg gtcaagttag gttgcgttcc agccgcgagg
     6601 gcgactacca ttgtctcgcc gacagcacgg gagaatccta agacgtaagc tgacgcgatg
     6661 cccgagaatg ccgccggcat aaggacgcgg agtgcggttt gtactcgtgt ggcccccatg
     6721 gcgtaggagc cctcacggat cgtcaccggt accgctttca tcgcgtcctc actgagcgaa
     6781 ctgacgtagg gaacgatcat gatgccgatg acgatgccgg cgcttaacat gttgaagccg
     6841 ggcaattcgg gcagtatgtt ttgcagcata ggtgtaacaa agagcagcgc aaaatagccg
     6901 aatactactg tgggtacggc gctcagtaac tcaaggattg gctttaaaat ttcccgaacc
     6961 gtggagttgg cgtattcgct caaatagatc gcaatgatag tccccagcgg cagcgcaacg
     7021 accagagcga tcattgaggt aacgagagtt ccggttacca gcggcaggat gccgtagcgc
     7081 gggttttcaa atagaaccgt ccattgcgta tcggtgagga aatcgatgag cgagacgttc
     7141 ctgaaaaaat tccaagactc atagaccagg acccaaacaa ttcccacggt gacggccact
     7201 gagaaaaccg cacaaaggaa cattccgcct tcgattatcc gctcgcgtat tttccgtgat
     7261 gcgacgcttc ccgagtgact gcgaatccgt tcccggacat ccgtataatc gatggcaact
     7321 tcagattttg acatgatgca ttgctttcac gttagggatt ttcggcagga gcgccgccgc
     7381 ccctgccgaa aggatcgtca cttagaagtt cgcttcgcgt ttcattaaat cctctatctt
     7441 gactccgact tcggcggtgc cgccaaagac ggtgccgatc ttccctttgg ttacatgctc
     7501 cgcagcgctc ttataaacgc ttgcaggcag ggcgacgtat ttcacatctt tcaccagctt
     7561 tgccgcattt tttagataga aatcgatgaa ctcttttacc tcgggcttac tgtccaagga
     7621 tttcttgctg acgtaaatga agatcggccg agataacggt tgataagtac cgtcttccac
     7681 ggtcttcggc gatggtccga cagctccttt accgccatcg atggccactg ctttcaactt
     7741 ggcttgattc tcgatgtaat aagcaaaacc aaagtaaccg agggcgttgc ggtcattgat
     7801 tacgccttga accaggacgt tgtcatcttc actggcg
//
LOCUS       DASQKS010000195         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_520900, whole genome shotgun sequence.
ACCESSION   DASQKS010000195 DASQKS010000000
VERSION     DASQKS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520900"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VE170_05085"
     CDS             complement(<1..209)
                     /locus_tag="VE170_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14841.1"
                     /translation="MAQKHVDSFGTRKVLTVDGESYDIFRLDQLEKAGFPNVSRLPVS
                     LKVLLENLLRQEDNHHVTKADIEALA"
     gene            complement(290..>2906)
                     /locus_tag="VE170_05090"
     CDS             complement(290..>2906)
                     /locus_tag="VE170_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ14842.1"
                     /translation="IANLLTLGLTAVLVLSISVMSARLARCHGEAESAAPSHLIGLFS
                     GSSARKRTEEALRANEERYRLTFEQAAVGISHVGLDGRWLRVNQKVCEITGYSPDEML
                     LLKAQDITHPDDLRLNLDLRAQLMAGEINTFSMEKRYVRKDRELVWVNLTASLHCDAS
                     GAPSHFISIIQDIGDRKRAEDALRQAHDELERRVDLRTAELAEAVVALTKTEDRLRQA
                     VDVADIGIFEHDHMADTEYCSPTLRRLIDLPEDRHGRVGDFFSQLHPEDREIVNLARR
                     QALDPAGDGRLSFECRVVRRDGTIGWILNRAETFFDGSGTARRPVRTVGALLDITERK
                     RSEEALRASEERLQHALAVGRMGTWERNLRTGEMIWDDRDFEIFGIAKDGAISRDLFL
                     ARVHPADLPVMEAALSRYERDGQDYQCEFRFLRPDGKMIWLSAHAGLRRDAKGVPTHL
                     TGITYDITERKAAEDALRNLNEQLDRRVARRTRELAESRARLRALVAELTMTEERERR
                     RLAVDLHDTLAQSLAVVNMHLWRVRELLGDQANGASFREVLNSLDTTVDSSIKYARSL
                     IAELSPPVLYDLGLPAAFRWLGEQMGQHGLRVEVDGPADGFSLAQDDAVFLFQCAREL
                     LWNVVKHGATDRATVAYGCDGDCVSLAVSDTGKGFDAQIARANGDDRSHFGLFSIRER
                     VALRGGTVEIKSTPGSGTWVLTVIPMHRPAVASAERVAPTIWPSATAPVIEQAIKILI
                     VDDHKMLRQGLRRVLEEQGGFNVIGEAGDGEEAVALARALQPQVVIMDVNMPNMNGIE
                     ATRLITQQLPSAIVIGLSFGTEDSVVRAMQAAGAVTCLPKERAVEDVSRAITDAATAR
                     WHRDPPSANFNPGIN"
BASE COUNT          576 a          919 c          817 g          594 t
ORIGIN      
        1 gcgagcgctt caatgtcggc cttggtgacg tggtggttgt cttcctgcct gagcaagttc
       61 tccagcaaga ctttcagcga caccggcaag cgcgagacat tggggaatcc cgccttctcc
      121 agctggtcga ggcgaaagat atcgtagctc tcgccgtcga cggtcaacac cttacgcgtg
      181 ccgaaactgt ccacatgctt ctgggccatg agcactcctt aactaaagat agggtaggcc
      241 ctattttgta gcgagcgtca cgggataaag caacagggag agtggcgagt taattgatgc
      301 ccgggttgaa gttcgctgac ggaggatcgc gatgccaccg cgccgtcgcc gcatccgtga
      361 ttgctcggct aacatcttcg acggcacgtt ctttcggcag gcaagtcacc gctccagccg
      421 cctgcatcgc ccggaccaca gaatcttcgg tgccgaagga taggccaata acgatcgccg
      481 acggcagttg ctgggtaatc aacctagtcg cctcgatgcc gttcatgttg ggcatgttga
      541 cgtccatgat caccacttgc ggttgcagcg ctcgcgccag tgccaccgct tcctcgccat
      601 cgccagcctc gccgatgacg ttgaagccgc cctgctcttc caacacccga cgcaagccct
      661 ggcgcagcat cttgtgatcg tcgacgataa gaattttgat cgcctgttcg atgactggcg
      721 ctgtcgcgct cggccatata gtcggcgcca ccctctcggc cgaagccacc gccggtcgat
      781 gcatcggtat gaccgtcaac acccaagtgc cgcttcccgg agtcgatttg atttcgaccg
      841 tcccaccgcg cagcgccacc cgctcgcgga tgctgaacag gccgaaatgg ctgcggtcgt
      901 cgccattagc acgagcgatt tgcgcgtcaa accctttgcc ggtgtcgctc accgcaagag
      961 acacgcaatc gccatcgcat ccatatgcga ccgttgcgcg gtcggtggcg ccatgtttga
     1021 cgacgttcca taacagttcg cgcgcgcact ggaacagaaa taccgcgtca tcctgcgcca
     1081 gcgagaagcc atcggccggt ccgtcgacct cgacgcgcaa gccatgctgg cccatctgct
     1141 cgccgagcca gcggaacgcc gccggcaaac ccagatcgta gagcaccggc ggactcaact
     1201 cggcgatcag cgagcgtgcg tacttgatcg agctgtcaac cgtggtgtcg aggctattta
     1261 agacttcccg gaacgaagcg ccattggcct ggtcgcccag cagttcgcgc acgcgccata
     1321 ggtgcatgtt gaccactgcc aacgactgcg ccagggtgtc gtgcagatcc accgccaggc
     1381 ggcgccgctc gcgctcctcg gtcatcgtca attcggcgac cagcgcacgc agccgggcac
     1441 gcgactcggc aagttctcgc gtgcgccgcg cgacccgccg gtcgagttgc tcgttcaagt
     1501 tccgcagcgc gtcttccgcc gctttacgct cggtgatgtc atacgtgatg ccggtcagat
     1561 gcgtcgggac gcctttggcg tcgcgcctga ggccggcgtg agcgctcaac cagatcatct
     1621 tgccatcggg ccgtaaaaag cggaattcac actggtaatc ctggccgtcg cgctcatatc
     1681 tgctaagcgc tgcttccata acgggcaggt ccgcgggatg cacacgggca agaaacaaat
     1741 cacggctgat cgccccgtcc ttggcgattc caaatatttc gaaatcccgg tcgtcccaaa
     1801 tcatttctcc ggtgcgcaaa ttccgctccc aagtgcccat acgtcccacc gccagcgcgt
     1861 gttgaagccg ttcctcgctg gctcgcagag cttcttcgga ccgtttacgc tcggtaatgt
     1921 cgagcaaggc accgacggtg cgcaccggtc tgcgagcagt gccgctgcca tcgaaaaagg
     1981 tttccgcccg attcaagatc cagcctatgg ttccatcgcg ccgcactacg cggcattcaa
     2041 atgacaagcg cccatcgccg gctggatcaa gggcttgtcg ccgcgccagg ttgacgattt
     2101 ccctgtcttc ggggtgtagt tgggaaaaaa aatcgccgac gcggccatgc cgatcttcgg
     2161 gcaagtcaat taatcttctt aaagtcggcg agcaatattc cgtgtctgcc atatgatcgt
     2221 gctcgaagat gccaatgtcg gcgacatcga cagcctgacg cagccgatct tcggttttgg
     2281 ttaacgctac gaccgcttcg gccaactccg cggtccgtag gtcgacgcgc cgctcgagct
     2341 cgtcgtgcgc ctgacgcaac gcgtcttcgg cgcgcttgcg atcgccaata tcctgaatga
     2401 tgctgataaa atggctcggg gcgccagacg catcgcaatg cagtgaggcc gtcaaattga
     2461 cccacacaag ctctcgatcc ttacggacat aacgtttttc catggaaaac gtattgatct
     2521 caccggccat caattgcgcc cttaaatcca agttgagtcg gagatcatcg gggtgggtaa
     2581 tgtcttgagc cttaagcaaa agcatttcat cgggcgaata tccggtaatt tcgcacacct
     2641 tttgattcac ccgtaaccaa cggccgtcta atcccacatg cgatatgccg accgcggctt
     2701 gttcgaaggt gagccggtag cgttcttcgt tcgcgcgcag cgcctcttcg gtgcgcttgc
     2761 gggcactgct gccggaaaat aagccgataa gatggctcgg cgcggcggat tcggcttcgc
     2821 cgtggcagcg agccaaccta gcggacatca cgctgatcga cagaaccagc acggcagtca
     2881 acccgagggt gagcagattg gcgatt
//
LOCUS       DASQKS010000196         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_521242, whole genome shotgun sequence.
ACCESSION   DASQKS010000196 DASQKS010000000
VERSION     DASQKS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521242"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..423)
                     /locus_tag="VE170_05095"
     CDS             complement(4..423)
                     /locus_tag="VE170_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HYJ14843.1"
                     /translation="MEIADAQKFIKDNHRACIAVRQKDGWPQMTLVTPGIDAAGRVIL
                     TSRGTTYKVKHLRRDPKVSMLIFGEQFSGSKFVQIHGSAEVIDLPQAMDGLIDWYKTV
                     RGEHKNWEDYKKQVTDEKRVLIRINIEKVGPQTVKRI"
     gene            complement(443..682)
                     /locus_tag="VE170_05100"
     CDS             complement(443..682)
                     /locus_tag="VE170_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14844.1"
                     /translation="MRTDSYNDHRIDFDSEEIRQGEWIARATIVFLDQRIEKRIPIFG
                     RRQATFDTQELADNYALELAKLWIDGRLEGTNGHG"
     gene            complement(787..1617)
                     /locus_tag="VE170_05105"
     CDS             complement(787..1617)
                     /locus_tag="VE170_05105"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14845.1"
                     /translation="MEKIGFVGTGAMGSGLLSRLKLAGVSAVSFDLTAKGQEAARAEG
                     AQTLNSAKEVAQACTIIDVVVRTDQEVLDCMLGENGVVEGAAPGSLVLLHSTIRPTTT
                     KKVAQAAADKRVNVIDACMTAVPSVVRQGGLTFLVGGQKAFFERAKPHLLNMAKDAVY
                     MGPLGCGNVTKLFKNMVTASEALVVYEALQIAKAGGIGYKAALDLMQKTRSAHILERW
                     ETRYQLEGNEMTFNAGTNLYDKDLPLAAEVGKALGADIPVVEALAALGIKLNGGKVSI
                     "
     gene            complement(1753..2763)
                     /locus_tag="VE170_05110"
     CDS             complement(1753..2763)
                     /locus_tag="VE170_05110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ14846.1"
                     /translation="MDYGIFDHLDRNSLPLNEFYEARLKLIEAYERAGFYAYHVAEHH
                     STPIGMAPSPSVFLSAIAQRTKRLRFGPMVYALPLHHPLRLIEEICMVDQLSGGRLEI
                     GFGRGSSPTENALYGQDPALAQAMYTEGRELILQGLTQKTLNYEGDHYKFKDVPMELE
                     PLQKPHPPMWYGVHSVEAAERAAQQNLNMVSLDSAQDTRSFNDKYREVWRALHGAKPT
                     GKLGLSRFIVIADTDQEALTIARRAYPVWHTNFYFLARLSGRMPAHPRAPEFDRMMEI
                     GQAIAGAPATVRQFLQAQVDESAANYLVGQFAFGDLSLTESLRTVELFRNEVMAKLTS
                     TN"
     gene            2886..3035
                     /locus_tag="VE170_05115"
     CDS             2886..3035
                     /locus_tag="VE170_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14847.1"
                     /translation="MEKPPIRKAYFEAECGCRQWYESVAGGPVEELEEVVEYCELHDI
                     ESGSA"
     gene            complement(3108..>3304)
                     /locus_tag="VE170_05120"
     CDS             complement(3108..>3304)
                     /locus_tag="VE170_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14848.1"
                     /translation="ERCTLSSDAGEPLFPNSVESLRLLRGHMSAFGCTDEEIYTMCTV
                     NPAFIVGLEVPRQQQSHATH"
BASE COUNT          630 a          922 c         1002 g          750 t
ORIGIN      
        1 gaatcaaatt cgctttaccg tctgcggccc gactttttcg atgttgatgc ggatcagcac
       61 gcgtttttcg tcggtcacct gtttcttgta gtcttcccag tttttgtgct cgccgcgcac
      121 ggtcttgtac caatcgatca gcccgtccat cgcttgcggt agatcgatga cctcggcgct
      181 gccgtggatc tggacaaatt tcgagccact gaactgttca ccgaagataa gcatcgagac
      241 ttttgggtcg cggcggagat gcttgacctt ataggtcgtg ccgcgggagg tgaggatcac
      301 ccgccctgcg gcgtcgatgc cgggagtcac cagggtcatt tgcggccagc cgtctttttg
      361 ccggacggcg atacaggcgc ggtggttgtc tttgataaat ttctgcgcgt cggcgatttc
      421 catctactcc tccgttgaga ctttagccgt ggccgttggt gccttcgagc cggccgtcga
      481 tccacaactt agccaattcg agggcgtagt tgtcggccag ttcttgggtg tcgaaggtcg
      541 cttggcgccg gccgaatatc ggaatgcgtt tttcgattct ctggtcaaga aacacgatag
      601 tcgccctggc gatccattca ccctggcgga tctcttccga gtcgaaatcg atacggtgat
      661 cgttgtaaga atctgtgcgc atgagcgtcg taatgttgaa tcgggcaggc cgcgggataa
      721 aaattcgcta ggtgattggc tgatgaatcg tttagccgag cttcattctt ccgcgtttgc
      781 ttcttttcag atgctcacct taccgccatt cagcttaatt cccaatgccg cgagcgcttc
      841 gaccacggga atatcggcgc ccagcgcctt accgacttca gccgcaagcg gcagatcctt
      901 atcataaaga ttggtgccgg cattgaatgt catctcgttg ccttcgagtt gataacgggt
      961 ttcccagcgt tcgaggatat gggccgagcg agttttctgc atgagatcaa gggcggcctt
     1021 gtagccgatg ccgccggcct tggcgatctg cagcgcttcg tagacgacca gggcttccga
     1081 tgccgtgacc atgtttttga atagtttcgt gacgttgccg cagccgagcg ggcccatgta
     1141 cacggcgtcc ttggccatgt tcaacaaatg cggcttggcg cgctcgaaaa aggctttttg
     1201 cccgccgacg aggaaggtca gcccgccttg gcgcacgacg ctgggcaccg cggtcatgca
     1261 ggcgtcgatg acgttgaccc gcttgtcggc ggcggcctgg gcgacttttt tagtcgtggt
     1321 cggacggatc gtgctgtgga gcaagaccag cgagcccggc gcggcgcctt cgaccacgcc
     1381 attttcgccg agcatgcagt cgagcacttc ttgatcggtg cgcacgacga cgtcgataat
     1441 cgtacacgcc tgggcaacct ccttcgccga attcagcgtc tgggcgccct cggcgcgcgc
     1501 ggcttcctgc ccctttgctg tgaggtcgaa gctgactgcg gagacgccgg ccaatttcaa
     1561 acgcgacagc agcccgctgc ccatggcgcc ggtgccgaca aagccgattt tttccataac
     1621 tgctccctcc gtagattatt gttagaattc ctgctcatcg ccgagacgcc gagacaattc
     1681 ttggaactct cggcgacctt cgcgccgcgg gatgaatcaa tcgattgttt aaatgatttt
     1741 cttataggat ttctaattgg tcgatgtcaa tttcgccatc acttcgttgc ggaacagttc
     1801 caccgtgcgc agcgattcgg tgaggctgag atcgccgaag gcaaattggc cgaccagata
     1861 gttcgccgcc gattcatcga cctgggcttg gagaaattgc cgcacggttg ccggcgcgcc
     1921 ggcaatcgct tggccgatct ccatcattcg atcgaactcc ggtgcgcgcg gatgggcggg
     1981 catgcggccg cttaggcgcg ccaggaaata aaaattcgta tgccacaccg gataggcacg
     2041 gcgcgcaatc gtcaacgctt cctggtcggt gtcggcgatg acgatgaagc ggctcaagcc
     2101 gagtttgccg gtgggcttgg cgccgtgcag cgcgcgccag acttcacgat acttgtcgtt
     2161 gaagctgcgt gtgtcctgag ccgagtcgag gctgaccata ttgagattct gttgcgccgc
     2221 gcgctcggcg gcctcgacgg agtggacgcc gtaccacatc ggcggatggg gcttttgcag
     2281 cggctcgagt tccatcggca cgtctttgaa cttgtaatgg tcgccctcgt agttgagggt
     2341 tttttgtgtc agcccctgga ggatcagctc gcgcccctcg gtgtacatcg cttgcgccaa
     2401 cgcggggtcc tggccatata gcgcgttctc ggtcggtgac gagccgcggc cgaagccgat
     2461 ctccaaccgt ccgccgctca attgatcgac catgcaaatc tcttcgatca ggcgcagcgg
     2521 atggtgcaag ggaagggcat aaaccatcgg gccgaagcgc agtctcttgg tccgctgcgc
     2581 gatggcggaa agaaatacgc tgggcgaggg ggccatgccg atgggggtcg aatggtgttc
     2641 ggccacgtga taggcgtaaa agcccgcgcg ttcgtaggcc tcgatcaact ttagcctggc
     2701 ttcgtagaat tcgttcagag gaagggagtt gcgatccaga tggtcgaaaa tgccgtaatc
     2761 cataccacgc cggatatatc gtgaatcggc gggaaatcaa agtcgccggc cgacatttgt
     2821 ccagttagcg tcgtcgatgc tatgaaggag cgtcgataga gctatcgcga gaagaaggat
     2881 aacccatgga aaagccgccg atcagaaaag catacttcga agccgaatgc ggctgccggc
     2941 agtggtacga gtccgtggcc ggcggtccgg tggaagagtt ggaagaagtc gtcgaatact
     3001 gcgagctgca tgatatcgag agcggcagcg cctaagtcca tttattctaa cactccagcg
     3061 cgcggtcgct gccaaccgcg cgctgaagca ttagcgtcca cttatgccta atgggtggcg
     3121 tgggactgct gctgccgcgg cacttctaag ccgacgatga atgccgggtt cacggtgcac
     3181 atggtgtaga tctcctcgtc cgtgcagccg aaggccgaca tgtggccgcg caagaggcgc
     3241 agcgattcca ccgaattggg aaatagcggc tcaccggcgt cggaagataa agtacagcgc
     3301 tcgg
//
LOCUS       DASQKS010000197         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_101429, whole genome shotgun sequence.
ACCESSION   DASQKS010000197 DASQKS010000000
VERSION     DASQKS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101429"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VE170_05125"
     CDS             complement(<1..1415)
                     /locus_tag="VE170_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ14849.1"
                     /translation="MAEYQLPPDGLIGSPERPPFSKEAKADVGGDARAIDHFLKGQLL
                     LTEGEFEPALKEFEAAALANPEDSFLHFRLAQLYLRRGDLRKALIEAESATRLESKNI
                     DYHLLVAGLHSALGDNQKGLGEYNEVLKLDPKHQEALLYAGALYLQVEDYARATQHLE
                     ELLKLDDSSALGHYYLGRVRAKSKLYLAAEQSFRRALELNSKSEAVLMDLALVYELEG
                     KTEDAIQTYQRLLQINPQHAWSRRRLGELYVGQNKLDEALRQFERLEGSDTDPRDTRI
                     KIGLISFEKGDYDRAATEFNLVLAGDPENDRARYYLAATYIELKANDKALEAFERISE
                     KSEFYADSRVQAAQLYERKDQTQKAIETLQTVIKKLNDRKEIHAYLSVLYRKTKDFPR
                     AIQSMERVVALDPRNDEAHFQLGALYDENKVKDKSIASMKKAIEINPQNAPALNYLGY
                     TWAEMGVQLDEAEDLIARALK"
     gene            1742..3760
                     /gene="ligA"
                     /locus_tag="VE170_05130"
     CDS             1742..3760
                     /gene="ligA"
                     /locus_tag="VE170_05130"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYJ14850.1"
                     /translation="MADTAHDRAAKEVIELRQALETHNYHYHALDNPLVSDAEYDRLF
                     RRLVQLEKEFPDLASADSPTLKVGAAPLAKFTTVQHALPMLSLNNAANGEELKEFEER
                     NQRFLKHSEPLEYAVEPKIDGLAVELVYIDGKFTVGSTRGDGINGEDITLNLKTIRSL
                     PLILRAGAHKPPRQLEVRGEVFLGRDAFQKMNRERDEEGQPVFANPRNAAAGSLKQLD
                     STITAKRPLDIFCYGFGSVEGASFASYWDFRKRIHEWGLKPVPRGILCRGLKEVLDYR
                     DQMEAERDTLPYEIDGLVVKVNDTQLQQRLGQIARSPRWAIAYKFPPRQSTTRIVDIV
                     AHVGRTGTLTPVASLEPVPIGGVVVKSASLHNMDEIDRKDIRIGDTVVVERAGDVIPY
                     VVRVIEEKRSGAEKHFIMPSQCPVCGSEVFRETDEAAYRCVGIACAAKLKESLKFFGS
                     RGAMDIEGLGEKLIVQLVDRNLVHDVADLYSLTKEQLADLDRLAEKSAQNLLDALERS
                     KNATLPRCLTALGIRHVGEATAKLLAEHFGTLEKVVLASEEELVEIREIGPEVAKSIA
                     RFFQQESNRQVIEKLKKAGLQFRVEMKKAGTLTGATFVLTGGLEEMSRPEAQKRIEAL
                     GGRVSSSVSRNTTYVVAGADPGTKLKKAKELGVKILSEEELLELLRAG"
     gene            complement(3757..4080)
                     /locus_tag="VE170_05135"
     CDS             complement(3757..4080)
                     /locus_tag="VE170_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ14851.1"
                     /translation="MAAKLYVGNLAYAVTDDRLEQHFAQHGSVVSARVVIDKFSGPSK
                     GFGFVEMASDAEAAKALDALNGTDFDGRNIVVSEARPQAPRSSGAGGYAGGGARPPQR
                     DRDRY"
BASE COUNT          910 a         1244 c         1170 g          861 t
ORIGIN      
        1 attttgaggg cccgcgcgat taaatcttcg gcttcgtcca attgcacgcc catctccgcc
       61 caagtgtagc ccaagtaatt aagcgccggc gcgttttggg gattgatctc aatggccttc
      121 ttcatgctcg cgatggattt gtctttaact ttgttctcgt catagagggc gcccaattga
      181 aagtgcgcct cgtcgttcct gggatcgagg gcgacgaccc gctccatcga ctgaatcgcc
      241 cgcggaaaat ctttggtctt acggtaaagc acgctcagat aggcatgaat ttctttgcgg
      301 tcgttgagct tcttaatgac cgtctgcagc gtctcgatgg ccttctgggt ctggtctttg
      361 cgctcgtaga gttgcgcggc ctgcacccgg gagtcggcgt agaactcgct cttttcggaa
      421 attcgctcaa aggcttccag cgccttatcg ttggctttga gttcgatata ggtggcggcg
      481 agataatagc gagcgcggtc gttttccgga tcgccggcga gaaccagatt gaactcggtg
      541 gcggcgcggt cgtagtcgcc cttctcaaac gagatcagac cgatcttgat gcgcgtgtcg
      601 cgcggatcgg tgtcgctgcc ttccaagcgc tcgaactggc gcagcgcttc atcgagctta
      661 ttctgcccga catagagttc acccaagcgg cggcgcgacc aagcatgttg cggattgatt
      721 tgcagtagac gctggtaggt ttggatcgca tcttcggtct tgccttcgag ctcatagacc
      781 aaggccaggt ccatcaacac cgcttccgac ttggaattga gctccaaggc cctgcgaaag
      841 ctctgctcgg cggcgaggta gagcttgctc ttagcgcgca cccggcccaa ataatagtgg
      901 cccaaagcgg agctgtcgtc gagtttgagc agctcttcca aatgctgggt cgcgcgcgca
      961 tagtcctcga cctgcaaata gagcgcgccg gcgtagagca gcgcttcttg atgcttcggg
     1021 tccagcttta ggacctcgtt gtattccccc agcccctttt gattgtcgcc cagcgcggaa
     1081 tgcagtccgg cgacgagcag atggtaatcg atgttctttg actccagacg ggtcgccgac
     1141 tccgcctcga tcaaggcctt cctaagatcg ccgcggcgca gatagagctg cgccaagcgg
     1201 aaatgcagaa agctatcctc aggatttgct agcgccgcag cttcgaactc tttcaaagcc
     1261 ggttcgaact cgccctcggt gagtaaaagc tgtcccttga gaaagtgatc aatcgccctg
     1321 gcgtcgccgc cgacgtcggc cttggcttct ttgctgaagg ggggcctttc gggagagcca
     1381 atcaaaccgt cgggagggag ctgatattcg gccaccgctg tcgcacaacc ggataatatt
     1441 atcaaaaaaa aagccgtccg aagaccgggt atagccatcg ttgaaaaatg taacatgctc
     1501 acctgagggg gtcaatgtaa gagtgatcga tggacggcgt tcaacggcaa atttaacgtt
     1561 gcgggtaata tcttgtgatc gcccctacga gaaaatatac gcatagccag caccgacatt
     1621 gacgacacct gaaatcaccg tgaagcgcta ccgattcggc gtcttcattc gccgcgtcat
     1681 ctatgctaat tttcccggcg gcggcggcaa ctcgccgcgc caactttcga aaagtaaacc
     1741 tatggccgac accgcccacg accgcgccgc taaagaagtt atcgaactac gccaggcgct
     1801 tgaaacgcac aactatcact accatgcgct cgacaatcca ttggtgagcg atgccgagta
     1861 cgaccggttg tttcgccgcc tggtgcaatt ggaaaaggaa tttcccgatc tcgcctcggc
     1921 ggattcgccg acgctcaaag tcggcgcggc gccgctcgcg aaattcacca ccgtgcagca
     1981 cgccctgccg atgctttcgc ttaacaacgc cgcgaatggc gaagagctta aagagttcga
     2041 agaacgaaac cagcgctttt tgaaacacag cgagccgctc gagtacgctg ttgagcccaa
     2101 gatcgacggc ctcgcagttg aattggtcta tatcgacggc aaattcaccg tcggctcaac
     2161 ccgcggcgat ggcattaacg gcgaggacat cacgctcaat ctcaaaacca tccgttcgct
     2221 gccattgatc ttgcgcgccg gcgcgcacaa gccgccccgg caactcgaag tacgcggcga
     2281 agtatttctc ggccgcgacg catttcagaa gatgaaccgc gagcgcgatg aagagggcca
     2341 gccggtattc gccaacccgc gcaatgccgc ggccggctct ctcaaacagc tcgactctac
     2401 gatcacggcg aagcgcccct tggacatttt ttgctatggt ttcggcagcg tcgagggcgc
     2461 gtcattcgca agctactggg attttcgtaa gcggattcac gagtggggct tgaagccggt
     2521 gccgcggggc atactttgcc gcggcttgaa ggaggtctta gactaccggg atcagatgga
     2581 agccgagcgc gacactctgc cctacgagat cgacggcctg gtggttaaag tcaacgacac
     2641 ccaactgcag cagcgcctcg ggcagatcgc ccgctcgccg cgctgggcta tcgcctacaa
     2701 attcccgccg cgccaatcca ccacgcgcat cgtcgacatc gtcgcccacg tcggccgcac
     2761 cggcacgctt accccggtag ccagtctgga accggtgccc atcggcggtg tcgtcgtcaa
     2821 aagcgcgtcg ctgcacaata tggacgagat cgaccgcaaa gatattcgca tcggcgacac
     2881 cgtcgtcgtc gagcgcgccg gcgacgtgat cccttacgtc gtcagggtca tcgaagaaaa
     2941 gcgcagcggt gcggaaaagc atttcatcat gccgtcacag tgcccggtgt gcggctcgga
     3001 agttttccgt gagaccgacg aagccgccta ccgttgcgtc ggcatcgcct gcgccgctaa
     3061 attgaaagag agtttgaaat tcttcggctc ccgcggcgcc atggatattg aaggtttagg
     3121 cgaaaaactt attgtgcaac tggtcgaccg caatctggtc cacgatgtcg ccgatcttta
     3181 cagtctcacc aaggagcaac tcgccgattt ggaccgcttg gcggaaaagt cggcgcagaa
     3241 cttactcgac gccctagaac ggagcaaaaa tgccacgctg ccgcgctgct tgaccgcctt
     3301 gggcattcgc cacgtcggtg aagcaaccgc caaactgctg gcggaacatt tcggcacgct
     3361 cgaaaaagtt gtcctagcga gcgaggaaga gctcgtcgaa atccgcgaga tcggtcccga
     3421 agtagcaaag agcatcgcgc gttttttcca acaggaaagc aatcgccagg tgattgaaaa
     3481 actaaaaaaa gccggcctgc aattccgcgt tgaaatgaaa aaagccggga ctttgaccgg
     3541 cgccaccttc gtgctcaccg gcggactgga agaaatgtcc cgaccggaag cgcagaaacg
     3601 tatcgaagcc cttggcggcc gggtttcatc aagcgtcagc cgcaacacca cctatgtggt
     3661 cgccggcgcc gacccaggca ccaagctcaa gaaagccaag gagctgggtg ttaagattct
     3721 cagcgaagaa gaactgctcg aactactccg cgccggttaa taacgatcgc ggtcgcgctg
     3781 cggcggcctg gcgccgccac ctgcataacc gccggcgccg ctcgatcgcg gcgcctgggg
     3841 gcgcgcttcg ctgacgacga tattgcgtcc gtcgaagtcg gtgccgttaa gcgcatccag
     3901 cgctttcgcg gcttcggcat ccgacgccat ctcgacaaaa ccgaatccct tggacgggcc
     3961 gctgaattta tcgatgacga cccgcgcact gactaccgat ccatgctggg caaaatgctg
     4021 ctccaggcga tcgtcggtca cggcataggc gagattgccg acgtatagct tcgctgccat
     4081 cctggccccc ttttcgctaa cctgagaccc tgaaaaaaac gccgacaggc tacaaaagtc
     4141 tcttggcgct tgtcaatcct cttggtcttt actgtaaagc cgatt
//
LOCUS       DASQKS010000198         5126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5561874, whole genome shotgun sequence.
ACCESSION   DASQKS010000198 DASQKS010000000
VERSION     DASQKS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5561874"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VE170_05140"
     CDS             <1..950
                     /locus_tag="VE170_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HYJ14852.1"
                     /translation="ISISSIYRLKMAAALKKFAADASAEYFDAKQKRLAHAIVSGDHE
                     IAGQVTDELISSQQTLGDIYLKVVTPALEGVGESWCRGDIGVGEQKLATEIVIGQMDR
                     LRAHFAPPAKRLLYRVMVACVEGEQHYIGARMLADLCGARGWHVDFLGANVPNQALVE
                     MVHRRHPQVVGLSATMTQGLENVRWVIGELEKSSPDVRVVIAGQAIVDNPSVKNLSRR
                     CEIAEDIIIGVGSIARLLRADRPKSVLKEYQMVLARRVRDLRSKRGWTQEHLAKVTQV
                     ARACIIAVEGGKQNVSMDILVRMANALEVAPESLLSAQG"
     gene            975..2099
                     /locus_tag="VE170_05145"
     CDS             975..2099
                     /locus_tag="VE170_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYJ14853.1"
                     /translation="MKQQRLTRYSGSAPALRRIAPASEDKCSRPVNGGGAEPAMFADG
                     RLAEMLGLAPSAAVESALRSSLFDPMAALINNPGKRLRAQLVTMIYRLFHDDRSVSFA
                     AAKQCRDCAEIVELMHAGSLIVDDIEDGSQMRRGRPALHVQFGVPLALNAGNWLYFWP
                     FELLRETGFAKDQMLSAYERYNRTLLRAHFGQALDLSAKLPHMPQDSVAEVCIADMTL
                     KTGALMGFAAVLGGTVAGESDHVLEALDAFGVDLGIALQMYDDLGNVIGKGDPQKRYE
                     DLSLSRPSWAWACAANCTDAYEYESFLQAVAELPCIESLESWIASHDLIPSTRASARQ
                     YLDRAFDRLKQRLCESDIAWSSRAFNELRLLGEEIAVAYG"
     gene            2092..3624
                     /locus_tag="VE170_05150"
     CDS             2092..3624
                     /locus_tag="VE170_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HYJ14854.1"
                     /translation="MDKPRVAVIGSGFGGLAAAIRLQAAGCDTVIFEKRDQPGGRAYV
                     YRDNGFVFDAGPTVITAPHCLEELFTIAGKSMRDYVELIPVKPFYRLFWDDGYQFDYS
                     DDDDALEKQIRAKSPGDWGGYQRFLKYSQEVFHQGYEKLVHVPFLNLSSMAAVAPQLI
                     RLEAYRSVYSVVAKHIRDPHLRQLFSFHSLLVGGNPFTTTAIYTLIHCLERKWGIYFP
                     KGGTGQLVSALVTLFRELGGEIRFDVEVEQIVTRDGAVAGLQTSAGREESFDLVVSNA
                     DVVHTYDRLLKGEPRAAPMARKLGRSKYSMSLFLIYFGTDRAYPNLQHHNVIFGPRYR
                     ELLADIFERGRLADDFSLYLHAPTRSDPTLAPPGCEAFYVLSPVPHLGKLAIDWKVEG
                     PAYAAKILQYLEQRYMPGLATSIVTQRIFTPADFESELNAHHGSAFSLEPRLMQSAYF
                     RVHNRDRNIRGLYFVGAGTHPGAGIPGVVNSGKATANLVLEDMKSILASAAVKTAEVS
                     YA"
     gene            3617..4570
                     /locus_tag="VE170_05155"
     CDS             3617..4570
                     /locus_tag="VE170_05155"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HYJ14855.1"
                     /translation="MPDALTPEVRAALNDCRDIITKGSKSFSLAAKLFDAQTRDAAVF
                     LYGWCRYCDDQVDDAGKAEDRLELEARLKALNEKTASAFSFAPQQEPVFIALQHIVHR
                     YDIPAHYALELIEGMAMDVRGTQYRNLPDLLLYCYRVAGTVGLMMSHVMGLRDESALR
                     NAADLGIAMQLTNIARDITEDAGMGRIYLPLAWLDEANIPPDQISAPENRHKLAMLTL
                     RLLNEADRYYRSGDAGLWHLSFRSACAVSAARHVYSEIGSLLVKRGPRAWDQRTYVTG
                     ARKIWVVLRGVLTLLRSAPARLFQPWSPGALRTIWKYSNVQ"
     gene            4681..>5126
                     /locus_tag="VE170_05160"
     CDS             4681..>5126
                     /locus_tag="VE170_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYJ14856.1"
                     /translation="MSPNGVGADAYQVSLRRGRSGIDTISCFDAGALSCRVAGEVRNI
                     EAENILPAKDIKRTGRAVHLAVTATQEALRRARVCPAGMSLAEKQSWGVILGSGGGTA
                     DFVEEQYRLYYSDQLRKISAYNVSSSTIGSLSSEISIHFGFHGPSHV"
BASE COUNT         1017 a         1424 c         1538 g         1147 t
ORIGIN      
        1 gaatctcgat tagctctatt tacaggttga aaatggccgc tgccctgaaa aaatttgccg
       61 ccgatgccag cgccgaatat ttcgacgcaa agcaaaagcg cctggctcac gcgattgtca
      121 gcggcgatca cgagatcgcc gggcaggtga ctgacgaact tatctcatcg cagcagacgc
      181 tcggtgatat ttatctcaaa gtggtcaccc cggctctcga aggtgtcggc gagtcatggt
      241 gccgcggtga tatcggcgtc ggcgagcaga agctggcgac ggagatcgtg atcggccaga
      301 tggaccgact gcgcgcgcac ttcgcgcctc cggcaaagcg cctgctctat cgggtcatgg
      361 ttgcctgcgt cgaaggagag cagcattaca tcggcgcccg catgctggct gacttatgcg
      421 gagcacgggg ctggcacgtc gactttctcg gcgccaatgt gcccaaccaa gcgctcgttg
      481 agatggtgca tcgacgtcac ccacaggtgg tcgggctttc ggcgacgatg acgcaagggc
      541 ttgaaaatgt tcgttgggtg atcggtgagc ttgaaaagtc gtcgcccgac gtccgggtgg
      601 ttattgccgg tcaggcgata gtcgacaatc ccagtgtgaa aaatctcagc cggcgttgtg
      661 agattgccga agatataata atcggcgtcg gttcaatcgc ccgacttttg cgcgccgatc
      721 ggccgaagtc ggttcttaaa gaatatcaaa tggtgctggc gcgccgcgtg cgtgatttac
      781 gatcaaagcg gggatggact caagagcacc tggccaaagt gactcaggtg gcgcgcgcct
      841 gcatcattgc cgtcgagggc ggcaagcaaa acgtttccat ggatattttg gtgcgcatgg
      901 ccaacgcttt ggaagtggcg ccggaaagtt tgttgtcggc gcaggggtga gtgttcggac
      961 acgggagtaa cggaatgaag caacaacgtt taacgaggta cagcggctcg gcgccggcgc
     1021 tgcggcgaat cgcgcccgcg tctgaggaca agtgcagccg gccggtcaac ggcggaggtg
     1081 ctgaacctgc tatgtttgcc gacggtcgat tggcagagat gctcggcctg gcaccgagcg
     1141 cggcggtgga aagcgcgctg cgtagctcct tgttcgatcc gatggccgcc ctcattaaca
     1201 acccgggcaa acgcctgcgc gcccaactgg tcaccatgat ttatcggcta ttccacgacg
     1261 accgctcggt atcttttgcc gcggccaagc agtgccggga ctgtgccgaa atagtcgagc
     1321 tgatgcatgc cggctcttta atcgtcgatg acattgaaga cggcagtcag atgcgccgcg
     1381 gccggccggc gttgcacgtg cagttcggcg tgcctttggc gctcaatgcc ggcaactggc
     1441 tatatttctg gcccttcgag ctactcagag agactgggtt tgccaaggat cagatgctca
     1501 gcgcctacga gcgctataac cgcacgctgc tccgtgccca tttcgggcaa gccctcgatt
     1561 tgagcgccaa gttaccccat atgccgcagg attcggtggc ggaagtttgc atagccgata
     1621 tgaccctgaa gaccggcgcc ctgatggggt tcgcggcggt gctcggtggc accgtcgcag
     1681 gcgaatccga tcacgtgctt gaagccctcg atgcctttgg cgtcgacctc ggcatcgcct
     1741 tgcaaatgta cgatgacctg ggaaacgtaa tcggcaaagg cgatccgcaa aagcgttatg
     1801 aggacctatc gctgtcgcgc ccctcttggg catgggcttg cgcggcaaat tgcaccgacg
     1861 cttatgaata cgaaagcttt ctccaagcgg tcgccgagtt gccatgcatt gagtccttgg
     1921 aaagctggat cgcctctcat gatcttattc catcgacccg cgcgagcgcg cggcagtatc
     1981 tggatcgcgc cttcgatagg ctgaagcagc gcttgtgtga gtcggacata gcgtggtcat
     2041 cgcgagcctt taacgaattg cgtcttttgg gcgaggagat tgcagtcgct tatggataag
     2101 ccacgcgtcg cagtcatcgg tagcgggttc ggcggattgg ccgcggctat tcgcctgcag
     2161 gcggccggct gcgacaccgt cattttcgaa aagcgcgatc agcctggcgg gcgcgcctat
     2221 gtctaccgtg acaacggttt tgtcttcgat gccggcccga cggtgatcac cgcgccgcac
     2281 tgtctcgagg agctgttcac gatcgccggc aagtcgatgc gggattacgt cgagctgatt
     2341 ccggtcaagc ctttttaccg tttattttgg gacgatggtt atcagttcga ttactcggat
     2401 gacgatgatg ccctagaaaa acaaattcgc gccaaatcgc cgggcgactg gggaggttat
     2461 caaagatttc taaaatattc ccaagaggtg tttcaccaag gatacgagaa gctcgtccat
     2521 gtgccgttct taaatctgtc gagtatggcg gcggtggcgc cgcagttgat ccgcctcgag
     2581 gcctatcgtt cagtctacag cgtggtcgca aaacatattc gcgatccgca tctgcgccag
     2641 ctgtttagtt ttcactcgct tctggtaggc ggcaacccgt ttacgaccac ggcgatttat
     2701 actctgattc actgtctcga gcgcaagtgg ggcatttatt ttcccaaggg cggcacgggc
     2761 cagctcgttt cagcgttggt cactctgttc cgagaactcg gtggcgagat tcgcttcgat
     2821 gttgaggttg aacaaatcgt tacccgtgac ggcgcagtgg ccggtttgca gaccagcgct
     2881 ggccgggaag aaagtttcga tctggtggtg agtaacgccg atgtcgtcca cacctatgat
     2941 cgactgctga aaggcgaacc gcgcgccgct ccgatggcac gaaagcttgg ccgtagtaaa
     3001 tacagcatgt cgttgtttct catttacttc ggcaccgacc gggcataccc caacttgcag
     3061 caccataacg ttatcttcgg cccgcgctac cgtgaactct tggccgatat tttcgaacgt
     3121 ggcagactag ctgacgattt ttcgctttat ttgcatgcgc cgacccgatc cgatccgacg
     3181 ctggcgccgc cgggctgcga agccttctac gtgctttcgc cggtgccgca tctgggaaag
     3241 cttgcgatcg actggaaagt cgagggaccg gcttacgcgg caaagatttt acagtatctg
     3301 gaacagcgct atatgcccgg tctcgccacg tcgattgtca cgcagcgaat ttttaccccg
     3361 gcggattttg agagcgagct caacgcccat catggctcgg cattttcctt ggagccgcgc
     3421 ctgatgcaga gcgcctattt tcgcgtgcac aatcgcgacc gaaatattcg cgggctctat
     3481 ttcgtcggcg ccggcaccca tccgggggcg ggtattcccg gtgtggtcaa ttccggcaag
     3541 gcgaccgcca atttggttct tgaagatatg aaatctattt tggcaagcgc ggctgtcaaa
     3601 accgcggaag tttcctatgc ctgatgcgct aacccccgaa gtacgcgcgg cgctcaatga
     3661 ttgccgcgac attatcacca agggctcgaa aagtttctcc ttggcagcga agcttttcga
     3721 tgctcagacg cgcgacgcgg cggtgtttct ctacggttgg tgccgttatt gcgacgatca
     3781 agtcgatgat gccggtaaag cggaggatcg gcttgagttg gaagccaggc ttaaagcact
     3841 taatgaaaaa accgcttcgg cgttctcctt tgcgccgcag caagaaccgg tgtttatcgc
     3901 cctgcagcat atcgtgcacc gctacgacat accggcgcat tacgcgcttg aattgatcga
     3961 aggcatggcg atggatgtgc gcggcacgca ataccggaat ttgcccgacc tcttgctcta
     4021 ctgttaccgg gttgccggca ccgtgggttt aatgatgtcc catgtgatgg ggttgcgcga
     4081 cgaaagcgcg ctgcgcaacg ccgccgatct ggggatcgcg atgcagctca ccaatattgc
     4141 tcgcgacatc accgaggatg ccgggatggg gcgcatctat ttgccgctcg catggctcga
     4201 tgaagcaaac atccctcctg atcaaatcag cgcgccggaa aatcgtcata agctggcgat
     4261 gttgaccttg cggctgctca acgaagcgga tcgctactat cggtcggggg atgccggatt
     4321 gtggcattta tcgtttcgtt cagcctgtgc tgtgtctgcc gcgcgccatg tttattccga
     4381 aatcggctcc ctgctggtta aacgcggccc gcgcgcttgg gaccaacgga cctatgtcac
     4441 cggcgcgcgc aagatttggg tggtgctgcg tggcgtcttg actctcctgc gctccgcgcc
     4501 ggcgcggctg tttcaaccct ggtcgcctgg cgcgttgaga actatctgga agtattccaa
     4561 tgttcaatga gatcaagctt gatacgccat gccggccgcc gaaaggagtg gccgaattca
     4621 atgagccgtc acgaagctga atcaaaactc cgccgggtcg ttgtcaccgg agtcggagtc
     4681 gtgagcccga acggtgttgg cgccgatgcc tatcaagtgt ctctgcggcg gggcagaagc
     4741 ggtatcgata cgatctcctg tttcgatgct ggcgcgcttt cttgccgtgt cgccggcgag
     4801 gttcggaaca tcgaggccga gaatattttg cccgccaaag atatcaaacg caccggtcgc
     4861 gcggttcatc tggcggtcac ggcaacgcaa gaagcgttgc gccgcgcccg agtttgcccg
     4921 gcaggcatga gcttggcgga aaaacagtcc tggggcgtga tactcggcag cggcggcggc
     4981 accgccgatt tcgtcgagga acagtaccgg ctctattaca gcgatcagtt gcgaaaaatc
     5041 agcgcttaca acgtatcgag ttcgaccatc ggctcgctct cgagcgagat ctctatccat
     5101 tttggttttc acggccctag ccatgt
//
LOCUS       DASQKS010000199         9133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5562565, whole genome shotgun sequence.
ACCESSION   DASQKS010000199 DASQKS010000000
VERSION     DASQKS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5562565"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1222
                     /locus_tag="VE170_05165"
     CDS             125..1222
                     /locus_tag="VE170_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HYJ14857.1"
                     /translation="MPVTAPALDDDLDLASLRLAIVHSLAYLKKLPPDRVVGVQPRPV
                     TARELLDSLIAFDGLLAHSQCRACLARAIAAAFEIIPSSAGANEADVLFTGYYQPVID
                     GSLTPGDGFDYPIYGVPADLIAPEQVGANGAENDDKQPGSIEYELRVPYYSRREIDQL
                     GALRGRALEIAWVKDPIDLFFLHIQGSGLLRLRDGREVTVGYAAQNGLPYRSIGRLLI
                     DDGKVAKEEMSMQRLRRYLTENPSEQNDIFAYNESYVFFRTNQDGPLGSLGVPVTAGR
                     SIATDARLFPRGALALVQMDIPIIGTQGELAGWRPVTRFVLNQDTGGAIRGPQRADYY
                     FGTGDEAGALAGYMNRQGRLFFLVPKSSNRN"
     gene            1305..2177
                     /locus_tag="VE170_05170"
     CDS             1305..2177
                     /locus_tag="VE170_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYJ14858.1"
                     /translation="MARHTETQRLKWALIISSVYFFAELAAGFVTNSLALLSDAGHML
                     SDIGALALSAFAFRVAKRPATHHSTYGYHRVEILAALFNGLTLWLIVGVIFTSAYYRL
                     ANPPTVESFGMIVVAVLGLMVNLAAAVILHSGHQHNLNMRGAFLHVLSDAIGSVGAIA
                     AGVIMLTTGWYLADPLISMFIGALILYSSWSLIKDSLSVLMQSVPKGIRLDEVRQTIE
                     AVDGVYKVHDLHVWAVTSDIYTLSAHAVVENGGDFHLVLNGIEDTLKDRFNIEHTTIQ
                     LETESREDKEFKDF"
     gene            2339..3478
                     /locus_tag="VE170_05175"
     CDS             2339..3478
                     /locus_tag="VE170_05175"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYJ14859.1"
                     /translation="MKAKIILGISLAVLVLAGAGYSLRHVFGDRREFVISGIVEADDI
                     HVGSKVGGRVLKVAVKEGQAVKAGEPLVLLEPRELNAALAEAQASLRQSDAKFALLSA
                     GYRKEEIEQAQAATRQAQADLDQQVSGPRQQEIAQAAADWQAAKAQAENARKFLSRMD
                     DLAKRELIAKQEFDDAFTKSDEAEQKMKSARERYDLLLAGTRKEEVERARQRWAEADA
                     KRRQLRGGFRTEEITQAKSEMEAARARLEQIQTQLEETVIKAPVDALVEALDLQPGDL
                     IGAGKPVATLLRQGSLWVRAYLPEARLGFVQPGLKVKVRVDSFPNREFAGAVRRIHRQ
                     GEFTPRNIQTHEERVLQVFQVEVVIDDADHILRPGMSADVTIVTP"
     gene            3541..4491
                     /locus_tag="VE170_05180"
     CDS             3541..4491
                     /locus_tag="VE170_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14860.1"
                     /translation="MTIAIKADGIGKKFGALSAVSDVSFTIEEGEIFGFLGPNGSGKS
                     TMIRILCGLLQPTSGRAELDGLDVVAHTEEVKRRIGYMSQRFSLYEDLTVDENIDFYG
                     AIYGLTRAELAARRRAVAALVGLDDRLDQMAATLSGGYKQRLALACSLLHQPKILFLD
                     EPTAGIDPVARREIWDLLFRLAAEGTTLFVTTHYMDEAERCSQVAYIYLAKLIAIGRP
                     QDLKRLPEITPAGCAWVEVTSEDATQLLPLLRRHGDVVDATIFGEAIHALVRQSTDAA
                     SLIEFARQQGGVNVACRAVPPSLEDVFVSLTRMRRATEGR"
     gene            4488..5594
                     /locus_tag="VE170_05185"
     CDS             4488..5594
                     /locus_tag="VE170_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14861.1"
                     /translation="MMFSGLWAILKKEVIHIRRDPATRFIFAIPLFELLLFGYAIDTD
                     VKHVPTVVFDLNRQIESRQLVREFENTHYFRLVGEVGSDRELQDAIIGGRAKVGIKIP
                     PDYSTNLVNGRQGQVQVIIDGSDSTIATRVLNASQQLGFIKSLERDGLSPSQFSIDIR
                     PRLLFNPNLLSANFYVPGLIGIILQLVTVFLTAFAIVRERERGTLEQLLVTPVSKGGL
                     IVGKLLPYTAIGFGQTVLVLLLMVFLFGVPIHGSVALLLFLSMVFLVPALGMGIVIST
                     LARNQSEAMQMSLMTLLPAFLLSGFVFPRESMPLPIYLISFLIPATYYLEILRGIILR
                     GAGIVALWDEALVLAAFGLFFMILSVVRFKKHLG"
     gene            complement(5659..6882)
                     /locus_tag="VE170_05190"
     CDS             complement(5659..6882)
                     /locus_tag="VE170_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14862.1"
                     /translation="MHSIAAFFGLDKLAPEKARALSIVYAASLALMMGITFIQPALPA
                     LTQPFQVSDAQLSWIMTLFTAPAIFLSPVFGIIADLYGRRLLLAYGLMVFGLFGGAMA
                     LAPSYTWLLVFRTLQGIGFSAVIPLTIVLIGDLLDGDKEISGQGLKVFLDRIGYFIFP
                     PLGGLLATIAWFWPFVVYLLAIPLGLVALFCMPETKGKDKQPAANYLGDVLRLTRHPR
                     LMIAFSAGFLRFFLDYGFLTYLPLFLVRTHGISTATAGLLYVFFAIGAMATSSQAGRI
                     ATGRDKARVLFLAFVVSGASVVAVPFIPNVWLIGGALFFYGLANGVISPMQKSLLTQN
                     APAELRGGIVSFDRLIQQISKTTSTSIVGLLLVSTALPTIFWMLGILSFLSVALMGML
                     LPRGAKIVATPTYTA"
     gene            7097..8527
                     /locus_tag="VE170_05195"
     CDS             7097..8527
                     /locus_tag="VE170_05195"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ14863.1"
                     /translation="MSQDMRSWIDQLEKAGLLARITKPVDPRTQMGALLWQARDRGLL
                     FENLPGYPGWRCLGQAPGDVALAPVAFGCERDQMIPEFVRRTETLGKTRLVETGPVQQ
                     KVLLGDAVDITKMPIHQAGIRDGGPFIGSGLMITKNPETGQRNLSFHRLQMKEPKKTG
                     ILLYPRHAWANYQMYEAQDKPMPVAIMIGHHPMYYFAAATTTQYGVDEFEIAAALLQE
                     DVELVKCRTVDLEVPAWAEIVLEGEIPPKIREPEGPFSEFQDYYLTGSGQNPIVNIKA
                     ITMRNDAIFKNVQNGTEVEGCVYHKVPMSAQILRRARSVGGLADVRNVLVMPGIFGVV
                     VQMKPRYFGEAKNVLMSVLSSEYQHPKIAIAVDVDVDIFNYAEVLWSINTRVNPSEDI
                     VTIPGAKIHAMDPSCPEFGHPGAPGWHRVGGKMIIDATKPPECDAQRRKEFERLKPVG
                     WETVKLEDFLPAGAPPLRIKPKIPMD"
     gene            complement(8638..8883)
                     /locus_tag="VE170_05200"
     CDS             complement(8638..8883)
                     /locus_tag="VE170_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14864.1"
                     /translation="MTQVKKTANPKVTIIASKGANQAINYLLEDRDELDWLVAIGRNK
                     NGDIFFYDTGGDIIQDLGAIEYVKERIVRDYFGEPDE"
BASE COUNT         1932 a         2490 c         2784 g         1927 t
ORIGIN      
        1 acttcttcgc gaatgtctcg atgattcacc ggggttgagt ttcccgtaaa tgtttctgcg
       61 ctgcaagctc tcacaacttt tcctaacgtt aacggtggcg gtcgtcatag ccggcggctg
      121 tgcgatgccg gttaccgctc cggcgctgga cgacgacctc gatctcgcat cgctacgttt
      181 ggcaatcgtt cacagccttg cctatttaaa aaagttacca ccggaccggg tcgttggagt
      241 gcagccgcga cccgtcacgg cacgggagct gctcgactcc ttgatcgcat tcgatggatt
      301 gctcgctcac agccagtgcc gcgcttgcct agcgcgcgcc atcgccgctg ctttcgaaat
      361 cattccatcg agcgccggag cgaatgaagc ggatgtgctc ttcaccggct actatcagcc
      421 ggtgatcgac ggcagtttga cgccgggcga tggcttcgat tatccgattt acggtgtgcc
      481 ggcagatttg atcgcaccgg agcaagtcgg cgccaacggc gccgaaaacg atgacaagca
      541 gcccggcagt atcgagtatg aactgcgggt gccgtattat tcgcgccgcg agatcgacca
      601 actcggcgcc ctgcgcgggc gcgctctcga aatcgcctgg gtcaaagatc ccatcgattt
      661 gttctttcta catatccagg gatcggggct gctgcgcttg cgcgatggcc gggaggtaac
      721 cgtcggctac gcggcgcaga acggcttgcc ctatcgtagc atcggccgcc tgctaatcga
      781 cgatggcaaa gttgctaaag aagagatgtc gatgcagcga ctgcgccgtt acttgaccga
      841 gaatccgtcg gagcaaaatg acatcttcgc ttacaatgag agttatgtgt tctttcgcac
      901 gaatcaggat ggcccgctgg gaagtctagg cgtgccggtt accgccggcc ggtcgatcgc
      961 gaccgatgcg cgactgttcc cacgcggcgc gttggcgctg gtgcaaatgg acattccaat
     1021 catcggtacc caaggtgagc tggccggctg gcggccggtg acccgtttcg tgctcaatca
     1081 agataccggc ggggcgattc gcgggccgca acgggcggat tactattttg gcacaggcga
     1141 cgaggccggc gctttggcgg gatatatgaa ccggcagggg cggttgtttt tcttggtgcc
     1201 gaaaagcagt aaccggaact gacagagaca gtggcacgga ttattgtcga tgcgtcttga
     1261 gcctttctcc gcccgggggt aacctaacag cgatcagcga ttgcatggcg cggcatacgg
     1321 aaacacagcg gttgaagtgg gcattgatca tcagttcggt ctatttcttc gccgaattgg
     1381 ccgccggttt tgtcaccaat agtttggcgt tgctgtccga cgccggccat atgctttccg
     1441 acatcggcgc gttggcgctc agcgcgttcg ccttccgggt cgccaagcgg ccggcgaccc
     1501 atcacagcac ctacggctat catcgcgtcg aaattcttgc ggcgctgttc aacggcctga
     1561 cgctttggct gatcgtcggc gtgattttca cttcggccta ttatcgcttg gccaatccgc
     1621 ctacggtgga aagcttcggc atgatcgtcg tcgcggtttt ggggttaatg gtcaatctcg
     1681 ccgcggcggt gattctccac tccggccatc agcacaatct caacatgcgc ggcgcattct
     1741 tgcacgtctt gagcgatgcc atcggctcgg tgggtgccat cgctgccggt gtgatcatgt
     1801 tgaccaccgg ctggtacttg gccgatccgt tgatcagtat gtttatcggc gcgctgattc
     1861 tctactcgtc gtggagcctg atcaaagact ccctcagtgt gcttatgcag tcggtgccca
     1921 agggcatacg tctcgatgag gtgcgccaga ccatcgaagc ggtggacggc gtctacaaag
     1981 ttcacgacct gcatgtttgg gcggtgacct cggatatcta cacgttgagc gctcatgcgg
     2041 tggtagagaa cggcggcgac ttccatctgg ttttgaacgg catcgaggat actttgaaag
     2101 atcgcttcaa catcgagcat acgaccatcc aactggagac cgagagccgc gaagacaagg
     2161 agttcaagga tttttagcag cggttgcatc acgggcgctc gcaaggcgaa gctcgaaacc
     2221 aaaagagaat agagaatcac ccgctaggaa cgcaaagttc aaaaacggaa tcggatttgt
     2281 cgggagccgt tgaaaaccgc catgctggtt ttgggtggaa gagtttgtca ttacctccat
     2341 gaaagcaaag atcatactcg ggatcagtct agcggttttg gttcttgccg gcgccggcta
     2401 ttcgctgcga cacgtcttcg gcgaccgccg ggaattcgtt atttccggca ttgtcgaggc
     2461 ggacgatatt catgtcggtt ccaaggttgg cgggcgggtc ttgaaagtcg ccgtcaagga
     2521 agggcaggcg gtcaaggccg gcgagccgtt ggtgttgctc gagccgcgcg agctcaacgc
     2581 ggcgctggcc gaggcgcagg cgagcttgcg tcaatccgac gccaagttcg ctttgttaag
     2641 cgcgggctat cgcaaggaag aaatcgaaca agcccaagcc gctacacgtc aagcccaagc
     2701 ggaccttgac caacaagtct cgggaccgcg ccagcaggaa attgcccagg cggcggcgga
     2761 ctggcaggcg gccaaggcgc aggccgagaa cgcgcgcaag tttctcagcc gcatggacga
     2821 cttggccaag cgcgagctga ttgccaaaca agagtttgac gatgctttca cgaaatcgga
     2881 tgaagccgaa cagaagatga aaagcgcccg ggaacgctac gatcttttgc tcgccggcac
     2941 gcgcaaggaa gaagtcgagc gggcgcgcca gcgctgggcc gaagccgatg ccaagcggcg
     3001 ccagctgcgc ggcggtttcc gcacggaaga aatcacccag gccaagtcgg agatggaagc
     3061 cgcccgcgcg cggctcgaac aaatccagac tcaactcgaa gaaaccgtga ttaaagcgcc
     3121 ggtggatgcg ttggttgagg ctttggactt gcagccgggg gacctgatcg gcgccggcaa
     3181 gccggtggcg acgctgttgc gccaggggag tctgtgggtg cgcgcctatc tgccggaggc
     3241 gcgtttgggt tttgtccagc cgggattgaa agttaaagtg cgcgtcgatt catttcccaa
     3301 tagggagttt gccggcgcgg tgcggcgcat acatcggcag ggagaattca cgccgcgcaa
     3361 tattcaaacc cacgaagaac gggtgctgca agtgtttcaa gtcgaagtgg tcatcgacga
     3421 tgccgaccat atcttgcgtc cgggcatgag cgccgacgtg acgattgtaa ccccgtgacg
     3481 gagcccagtc ctggcgtggg actggattag cactgccgcg ccaattagca acggacgatc
     3541 atgactatcg cgatcaaagc ggacggtatc ggcaagaaat ttggcgcgtt gtcggcggtc
     3601 agcgatgtca gttttaccat cgaggaaggt gagatcttcg gattcctcgg ccccaatggc
     3661 tcgggcaagt cgacaatgat ccgcatcctc tgcggcctgt tgcagccgac ctcagggcgg
     3721 gccgagctcg acggtctcga cgtcgtcgcg cataccgagg aggtcaagag gcgaatcggt
     3781 tatatgtcgc agcgattcag tctctatgaa gatctcacgg ttgatgagaa tatcgatttc
     3841 tacggcgcga tttatgggct gacgcgcgcc gaactggctg cgcgccgacg ggcggtggcg
     3901 gctttggtcg ggctggatga ccggctggac cagatggcgg cgacgctgtc gggcggttat
     3961 aaacagcgcc tcgcgctggc ttgctcgctc ctgcatcagc ccaagatctt gtttctcgac
     4021 gaaccgacgg cgggcatcga tccggtggcg cggcgggaga tttgggattt gctctttcgg
     4081 ctggccgccg aaggcacgac gctgttcgtg acgacccatt atatggacga ggcggagcgc
     4141 tgctcgcagg tcgcctatat ttatcttgcc aagctgatcg ccatcggccg gccgcaggat
     4201 ttgaagcggc tgcccgagat cacgccggcg ggctgcgcct gggtcgaggt caccagcgag
     4261 gatgccactc agctgctccc gcttttacgg cgccacggcg acgtcgtcga cgcgaccatt
     4321 ttcggcgagg cgattcacgc tttggtgcgc cagtccaccg atgcggcgtc gctgatcgag
     4381 ttcgcccgcc aacagggcgg tgtaaacgtt gcatgccgcg ccgtgccgcc atctttggag
     4441 gatgtctttg tctctttgac gcgaatgcgc cgcgccacgg aaggacgatg atgtttagcg
     4501 gcctgtgggc aatcctcaaa aaagaggtga ttcacatcag gcgcgacccg gcgacgcgct
     4561 ttatcttcgc cataccgctt ttcgaattgc tgttgttcgg ctacgccatc gataccgacg
     4621 tcaagcacgt gccgacggtg gtgttcgacc tcaatcgcca aatagagagc cgtcaactgg
     4681 tgcgtgagtt cgagaacacc cactactttc gtctagtcgg tgaagtcggg tcggatcgag
     4741 agttgcagga cgcgatcatc ggcgggcgcg caaaagtcgg gattaaaatc ccgccggact
     4801 actcgacgaa tctggtcaac ggcaggcagg ggcaagtgca agtgatcatc gacggttcgg
     4861 actcgaccat cgccacacgc gtgctcaacg ccagccaaca gttgggtttc atcaaatcgc
     4921 tcgaacggga cggcctttcg ccaagccagt tcagcatcga tatccggccg cgcttactgt
     4981 tcaatccgaa tctcttgagc gccaactttt acgtgcctgg tttgatcggc atcattctcc
     5041 agttggtgac ggtttttctt accgccttcg ccatcgtgcg cgagcgcgag cgcggcacct
     5101 tggaacaatt actggttacg ccggtgtcca agggcgggct gatcgtcggc aagcttttac
     5161 cctacaccgc catcggcttc ggccagaccg tgctggtgct gttgttgatg gtttttctct
     5221 tcggcgtgcc gattcacggt tcggttgcct tgctgttgtt tttgtcgatg gtctttctcg
     5281 tccccgccct cggcatgggc atcgtgattt cgactttggc gcgcaatcag agcgaagcga
     5341 tgcagatgtc tctgatgact ttgctgccgg cctttttgct gtccggcttc gtttttccgc
     5401 gggagtcgat gcccttgccg atctatctga tcagtttttt gattcccgca acctactacc
     5461 tagaaatcct ccgcggcatc attttgcgcg gcgccggtat cgtcgcgctg tgggatgaag
     5521 cgctggtgct cgccgccttc ggcctgttct ttatgatctt gagcgtcgtg cgcttcaaga
     5581 aacacttggg ctaatcttcc tcgctagccc gcgcggatgc aaaattccag attgtagatt
     5641 aaacggtgag ctggatcgtc acgctgtgta ggttggagtc gcgacgattt ttgcgccgcg
     5701 gggcaacagc attcccatga gggcgacgct gagaaacgaa agaatgccta acatccagaa
     5761 tattgtcggc agtgccgtgc tgaccaacag caagccgacg atggaggtcg aggtggtttt
     5821 cgagatctgc tggatcaagc ggtcgaagga gacgatgccg ccgcgcagtt ccgccggtgc
     5881 attctgcgtc agcaggcttt tctgcatggg cgaaattacg ccattggcga ggccatagaa
     5941 aaacaaagcg ccgccgatca gccagacgtt aggaatgaac ggcacggcga cgactgatgc
     6001 gccgctgacg acgaaggcga gaaacaagac ccgcgccttg tcgcggccgg tggcgatgcg
     6061 gccggcctgg ctcgaggtcg ccatggcgcc gatcgcaaaa aagacataga gcaggccggc
     6121 tgtggcggtg gagatgccat gggtgcgcac caggaacaga ggcaggtaag tgagaaagcc
     6181 atagtcgaga aagaaacgca ggaagccggc gctgaatgcg atcatcagcc ggggatggcg
     6241 ggtcaagcgc aggacgtcgc ccaaatagtt tgccgccggc tgtttgtcct tacctttagt
     6301 ctcgggcatg caaaacagcg cgactagacc cagaggaatc gccaagagat aaacgacaaa
     6361 gggccagaac caggcgatgg tcgccagcaa tccgccgagc ggtggaaaga tgaaataacc
     6421 gatgcggtcg agaaaaactt tcagaccctg gccgctgatt tccttgtcgc cgtccaacaa
     6481 atcgccgatc aagacgatag tcagcggaat caccgcgctg aacccgatgc cttgaagcgt
     6541 gcgaaatacc agcagccagg tatagcttgg cgctaacgcc atggccccgc cgaacagacc
     6601 aaacaccatt aatccatagg ccagcagcag gcgccggccg tagaggtcgg cgataatgcc
     6661 gaacaccggc gataaaaaaa tcgccggcgc ggtaaacagc gtcatgatcc acgataattg
     6721 tgcatcgctc acctgaaacg gctgggtgag cgccggcaac gccggctgga tgaaggttat
     6781 gcccatcatc agcgccaggc ttgcggcata gacaatgctc aatgctctag ccttttccgg
     6841 cgcgagtttg tcgagaccga agaaggccgc gatggagtgc atgaagctga aagtaggcga
     6901 agcgtatgcg aaagacaagc tggacgactg gagtatggag tattggagta gccgaagatt
     6961 aaagtgttgg agtaatggag taatgggcag tccggaaacc caacactcca acactccagt
     7021 actccaattc ccgatggtgg gcgtagcggc cccgtgatat ggatgggcgc agtatcgagt
     7081 tgtggaggaa tctcttatgt cccaagacat gcgcagttgg atcgatcaac tggagaaagc
     7141 cgggctgttg gcgcgtatta ccaaacctgt cgacccgcgc acgcaaatgg gcgcgttgct
     7201 ctggcaggcg cgtgatcggg gcttactgtt tgaaaatctg ccgggttatc cgggttggcg
     7261 ttgtttaggg caggcgccgg gggacgtggc gttggcgccg gtggcgttcg gctgtgaacg
     7321 cgatcaaatg attcccgaat ttgtccggcg cacggaaact ttgggtaaga cgcggctggt
     7381 agaaaccggg ccggtgcagc aaaaagtttt actcggcgac gcggtcgata tcaccaagat
     7441 gccgattcat caggccggca ttcgcgacgg cgggccattc atcggctcgg gtttgatgat
     7501 cacgaaaaat cccgagaccg gccagcgcaa cctgagcttt catcggttac agatgaagga
     7561 accgaagaag accggcattt tgctctatcc gcgccacgcc tgggccaact atcagatgta
     7621 tgaagcgcag gacaaaccga tgccggtggc gatcatgatc ggccaccatc cgatgtacta
     7681 cttcgcggcg gcgacgacga cgcagtacgg cgtcgatgaa tttgagatcg ctgcggctct
     7741 gctgcaagaa gatgtcgagc tcgtcaaatg ccgtaccgtc gatcttgaag tccccgcctg
     7801 ggccgagatc gtgctcgaag gcgaaatccc gccgaagatt cgcgaaccgg aaggaccgtt
     7861 cagtgagttt caggactact atctcaccgg ctccgggcaa aatcccatcg tcaacatcaa
     7921 agcgatcacc atgcgcaacg atgcgatctt caaaaacgtc cagaatggca ccgaagtcga
     7981 aggctgcgtc tatcacaaag tgccgatgtc ggcgcagatc ctgcgccggg cgcgcagcgt
     8041 cggcggcctc gccgatgtgc gcaacgtgtt agtcatgccg ggcatctttg gtgtggtggt
     8101 gcagatgaag ccgcgctact tcggcgaggc gaaaaatgtc ttgatgtcgg tgctatcgag
     8161 cgagtaccag catcccaaga tcgccattgc agtcgatgtc gacgtggata ttttcaacta
     8221 tgcggaagtc ttgtggtcga tcaacacccg ggtcaacccg tcggaggata tcgtgacgat
     8281 acccggcgcg aagatccacg ccatggaccc gtcttgtcca gagttcggcc atcccggtgc
     8341 cccgggctgg catcgggtcg gcggcaagat gattatcgac gcgaccaagc cgccggagtg
     8401 cgacgcccaa cggcgcaagg aattcgaaag gctcaaacct gtgggctggg aaactgtgaa
     8461 gctagaggat ttcttgccgg caggggcgcc gccattgcgg attaagccga aaataccgat
     8521 ggattaaaat actggttcaa ggttcaaggg ttcaaatgtt caaggtcgga taaacataga
     8581 gttctgattg aaccctcgaa ctattgaacc cttgaactag ggggggcggg aaccgcgcta
     8641 ttcatccggc tcgccgaagt agtctctgac gattctttcc ttgacatatt cgatggcgcc
     8701 caggtcttgg ataatgtcgc cgccggtatc gtagaaaaag atatcgccat ttttattgcg
     8761 accgatggcg accagccagt cgagctcgtc gcggtcttcc aataaataat tgatcgcctg
     8821 attggcgcct ttgctggcga taatggttac tttcggattg gcggtttttt ttacctgggt
     8881 cattgagcgg cctcctggac cgaacggaag cttataacag ttgtgacggc taaacaactt
     8941 gcaagccggc aggggaacct gagaattttg cctcgtctat ttcatatttt aacaggcgcg
     9001 aaaaaattac ggcgtttatt ttcaagcaga tgcggcgctt agcaggtcgg agtcatttct
     9061 ttaagataaa tttcggacca gcacagcggc ggattcacaa aaccggctcg ctcgaaagca
     9121 gtgcctagtt ggg
//
LOCUS       DASQKS010000200         6514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3881787, whole genome shotgun sequence.
ACCESSION   DASQKS010000200 DASQKS010000000
VERSION     DASQKS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3881787"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VE170_05205"
     CDS             <1..632
                     /locus_tag="VE170_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14865.1"
                     /translation="PIRDVAESIKEVKRAARIGAAAVMLQGMAWDKLLGDESLFPFYQ
                     VAADLNLPVCVHLGWGCPALTRIFDATTNFYSAILPVLMGFNSIMSSAAFEKIPNLRF
                     GFLEAGAAWIPFMIKQVRRDNVRRKQAKDPIEYFTSGRIFVSTEPDEDLTGIAALVGE
                     GSFVLGSDYPHGDPSRQEDMVAEFRERKDLSQAMVEKMLCDNPKRLYGF"
     gene            complement(746..1078)
                     /locus_tag="VE170_05210"
     CDS             complement(746..1078)
                     /locus_tag="VE170_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYJ14866.1"
                     /translation="MPKRKHYKVHWTHAASVDLESNVDFIARKNVSNALDILTRLRKA
                     AANLKSFPLRGRVVPELAGIGVYFYREVISAPWRVIYRVADVDVYVMMVIDSRRNAED
                     LLLERFTR"
     gene            complement(1056..1361)
                     /locus_tag="VE170_05215"
     CDS             complement(1056..1361)
                     /locus_tag="VE170_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYJ14867.1"
                     /translation="MKISEDIRPVTYLKSRAADLLAQLNETHRPIVITQNGAARAVMQ
                     DPESYEQTRAAIGLLKLLIAGEKDIRSGNTEGQEELFNRLEKKLTRRKRVRAEKKTL"
     gene            complement(1625..2425)
                     /locus_tag="VE170_05220"
     CDS             complement(1625..2425)
                     /locus_tag="VE170_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ14868.1"
                     /translation="MPYSTSNGLKIYYEVSGEGLPFVMVHANPFDHNLWMYQIAHFST
                     YFKVVAIDIRGYGRSDKPETPFSLKDMADDVLGVCRDLDVKEAILGGVSVGSGMAILL
                     GLDHPEMFKAIVLVGGSSGPGGSIDERIRGYTKVGIEKYHIQHLRALVAPEFADSKLG
                     KYLLDTFVERDPWLSGESIGQIFRARGGLDMTPRLATMKVPTLVINGEYDNSLAGGKK
                     TASLIPGAVHKILPKTGHACNIEDPAAFDAFVTEFLRANNLMPSAANR"
     gene            complement(2579..3283)
                     /locus_tag="VE170_05225"
     CDS             complement(2579..3283)
                     /locus_tag="VE170_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HYJ14869.1"
                     /translation="MAADFEDHCWKDIVSPEILKTYTPYHRETFIGQRPALLAIDLYN
                     LAYQGGAKEPHELIDTYKSSCGVYAHNAIPPTQELFALARSLKIPVFYTTSETRVEAK
                     PDLINATNRQRAKIDPDAFEIKEEFKPQPGDVVIFKERASGFFGTPLVAHLTQLGCDS
                     LIVCGESTSGCVRASCVDGYSLGYHVSIVEECVFDRSQLSHKVNLFDMHHKYTDVMKL
                     DEVKEKIKQERPRKAN"
     gene            3433..5127
                     /locus_tag="VE170_05230"
     CDS             3433..5127
                     /locus_tag="VE170_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ14870.1"
                     /translation="MKKADNSSLTRRQFVKLTGATAAGALWLPPLFAAQPGRKRIVMH
                     GERQVTSLGYHNRRETEHVSMVDAGLVGQNPATFERVPVLAEELPSVKKGTWKIDAQK
                     KTMITVYKLRPNLKWHDGKPYTSKDFEFGWQIAKHPDFPMPDRLVPEMISKIETPDDR
                     TIVIHWNDLYNEAYAIQYTHVRAFPRHLLLDAFQGGDMKAFANLPYWNKNFVGAGAFK
                     VVEWDQGSRVELEAFNDFALGRPKIDRITYKTVEDSNTNLAAVMAGEVDVCMRSTISF
                     DGAMILRDQWEKQGKGKVLISPASWSWLSLSRDNPWFSDVRVRRALLHAIDRDAMVQN
                     LFKGEKVISHLPLSRARKVNNKALAASTIYKFDVERAKKLMAEAGWKAGADGVLANGK
                     GEKMEFEFRVTAERRDHEQAQAIISDYWKRLGVRTNIKNMPNRLLNSADNRNRWPGAY
                     IGSHNVTVEEWPERFHSKNIPTAENKYAPENVSGWNDPKKDAILDDINSIITPEQSEK
                     LQIEFCKMFSAALPHLPLFFTPEVLVVKKGLTGITPRQESGGQNSSSWNTQAWDKV"
     gene            5259..6230
                     /locus_tag="VE170_05235"
     CDS             5259..6230
                     /locus_tag="VE170_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ14871.1"
                     /translation="MLLYVVRRLLQTVPLLLVISALIFTLLYLMPGDPLYRMLEGIPN
                     LRPQDFDRLRKLYGFDDPVYIQYGKWLWQLLQLNPGYSREYGQPVFDIILPALKNTLV
                     LTIAAVVLGKIIAIALGIFSAIRQYSIADYILTATTFVAYSVPAFWLGLMLIILFSVK
                     LGWLPTSGIVNSELEPGSWAATVDWMQHLILPVAVLAISEIIQVQRFMRSSLLEVLRQ
                     DYLTTARAKGLRESVVIGRHALKNALIPVVTIIAVTMPRVVGGSTVVETVFAYPGMGR
                     LLYTSIMGNDYVVAMTVVMIIAVTVVFFNLLADVIYGWLDPRIRYRS"
BASE COUNT         1424 a         1706 c         1897 g         1487 t
ORIGIN      
        1 tgccgatccg cgatgtcgcg gagtcgatca aagaggtcaa acgcgccgcc cggatcggcg
       61 ctgccgcagt catgctgcaa ggcatggcgt gggacaagct actcggcgat gaaagcttgt
      121 ttccgtttta tcaggtcgcg gcggatttga acttgccggt ttgcgtgcac ctaggctggg
      181 gttgtccggc gttgacgcgc atcttcgacg ccacgacaaa tttctatagc gctatcctgc
      241 cggtgctcat gggcttcaac agcatcatga gctcggcggc gttcgagaag attcccaatc
      301 tccgcttcgg cttcctcgaa gccggcgccg cctggatacc cttcatgatc aagcaagtgc
      361 ggcgtgataa cgtgcggcgc aagcaggcca aagatcctat cgagtatttc actagcggca
      421 ggattttcgt cagcactgaa ccggacgaag atctgaccgg cattgcagcg ttagtcggcg
      481 aaggctcttt tgttctcggt tccgactatc cccacggcga tccgtcgcgc caggaggata
      541 tggtcgcgga attcagagaa cgcaaggacc tctcgcaggc gatggtcgaa aagatgctgt
      601 gcgacaatcc caaacgactt tacggatttt agggcaacgg cgagattccg attgctaggg
      661 tcgtcgactt ccaaccattg atcgaagtgc ggcgcgagtg gggtatgaaa taggaactca
      721 gtctacctgc ttcgatgagg cgagtttagc gagtgaatcg ttccagcaac aaatcttccg
      781 cgttgcgtct gctgtcgatc accatcatca cgtaaacgtc gacatcggcg acgcggtaga
      841 tcacccgcca tggcgcactg atgacctcgc gataaaaata gacacctatc cccgcgagct
      901 ctggaacgac cctgccgcgt agcggaaacg atttgaggtt cgcagcggct tttcgaagcc
      961 gggtgagaat atcgagagcg ttggagacgt ttttacgggc gatgaaatcg acgttcgatt
     1021 caagatcgac gctagctgcg tgagtccaat gaactttata gtgttttctt ttcggcacgg
     1081 acccgtttcc ggcgagtcaa tttcttttcc aagcgattga aaagttcttc ctgtccttcg
     1141 gtgttgccgg agcgaatgtc cttttcaccg gcaatcaaga gtttcaggag accgatggct
     1201 gccctggttt gttcgtagct ctcggggtcc tgcatgacgg cgcgagcagc tccattttgg
     1261 gtaattacga ttggccggtg ggtttcatta agttgggcga gcaagtccgc tgcgcgagac
     1321 tttaaataag tcaccggccg gatgtcttcg ctaattttca taaaatcctc acagtccgaa
     1381 taaagtacct tattgagccc gatctttcaa atacgaaatg ttcaaagccg acggcgccaa
     1441 ggccgcaaag gtgaccggcc gatgtccgtt atcccgagcg aaggcgaggg atctaaagaa
     1501 agatttctct ctgcggtcga aatgacaaca actccgaact tggcgtggtc gcgccttggc
     1561 gttccttcaa tgttgctcag ggtatgcttt gtcggtgata ttccggctct ggcgtccagg
     1621 cgagtcagcg attggcggcg gatggcatca aattgttggc acgcaggaat tctgtgacga
     1681 aggcatcgaa ggcggcggga tcctcgatat tgcaggcgtg gccggtcttg ggcaggattt
     1741 tgtgcacggc gccgggaatg agcgacgcag tttttttgcc gccggcaagc gagttgtcgt
     1801 attcgccatt gatgaccaag gtcgggactt tcatcgtcgc tagccgcggc gtcatgtcga
     1861 gaccgccgcg ggcacgaaag atttgtccaa tcgattcgcc cgacagccag ggatcgcgct
     1921 cgacgaaagt gtccaggaga tacttgccga gtttgctgtc ggcaaattcc ggcgcgacca
     1981 acgcgcgcaa atgctgaata tgatatttct cgataccgac tttggtgtag ccgcggatgc
     2041 gctcgtcgat ggagccgccg ggcccgctgc tgccgccgac gagaacaatc gccttgaaca
     2101 tttccggatg gtcgaggcct aataaaatcg ccatgcccga accgacgctg acgccgccga
     2161 ggatcgcttc ctttacgtcg aggtcgcggc atacgccgag cacgtcatcg gccatgtctt
     2221 tgagactgaa tggcgtttcc ggtttgtcgg agcggccata gccgcgaatg tcgattgcga
     2281 cgaccttgaa atacgtcgag aagtgggcga tctggtacat ccacaagtta tgatcgaatg
     2341 gattggcatg gaccatgacg aagggaaggc cttcgcctga aacttcataa tagatcttta
     2401 agccgttcga ggtgctgtag ggcattgagt cgctcagagt tcaaaacgtt caaccgcttc
     2461 cccatcgctg cgctctgggc tgcgctccgt tcaatccgtt caaatcgtta cggaggtttt
     2521 tccggtccga cattttgaac cacttgaacg ttttgaacgg cttgaacgag tttagccttt
     2581 agttggcttt cctcggccgt tcctgtttga tcttttcttt gacttcgtcg agcttcatga
     2641 catcggtgta tttgtggtgc atgtcgaaga ggttcacttt gtgggaaagc tggctgcggt
     2701 caaagacgca ttcttcaacg atggacacgt gatagccgag ggagtaaccg tcgacgcagc
     2761 tggcccggac gcagccgctg gtgctttcgc cgcagacgat caggctatcg cagcccaatt
     2821 gggtcaagtg cgccaccaac ggcgtaccga agaagccgct ggcgcgttct ttaaaaatca
     2881 ccacgtcgcc gggctgtggc ttgaactctt ccttgatctc gaaggcgtcg ggatcgatct
     2941 tggcgcgctg gcggttggtc gcgttgatca ggtcgggctt ggcttctact ctggtttcgc
     3001 tggtggtgta gaaaaccggg atcttcaagc tgcgcgccag ggcgaacagc tcctgcgtcg
     3061 gcgggatcgc gttatgcgca tacacgccgc aggaactctt gtaggtgtcg atcaattcgt
     3121 gcggctcctt ggcgccgcct tgataagcca gattgtagag atcgatggcc aacagcgccg
     3181 gcctctggcc gatgaaggtt tcgcgatgat agggcgtgta ggttttgaga atttccggtg
     3241 agacgatatc tttccagcaa tgatcttcga aatcagcagc catgggagcc tcctgtagat
     3301 tttcgcttcc catgtaactg gagcgctgcc ggattgtcaa ggaagccggt gggatatatg
     3361 cattgaccgc gccggagcca ttggttatac cggttgcgga aaacgtcgtg tcagttacgg
     3421 aggattcccc ccatgaaaaa agccgataat tcatcgttga ccaggagaca attcgtaaaa
     3481 ttgaccggtg ccacggcagc aggcgcgctg tggttgccgc cgttattcgc cgcccagccg
     3541 gggcgcaagc gtatcgtcat gcacggcgaa cggcaggtaa ccagtctcgg ttatcacaac
     3601 cggcgtgaga ccgaacatgt ctcgatggtc gatgccggac tagtcggaca gaacccggcg
     3661 accttcgagc gcgtgccggt gcttgccgaa gagttgccgt cggtgaaaaa gggcacctgg
     3721 aagatcgatg cgcaaaaaaa gaccatgatc acggtctaca agctgcggcc taatcttaag
     3781 tggcatgacg gcaaaccata tacctcaaaa gattttgagt tcggctggca gatcgccaag
     3841 catccggatt ttcccatgcc ggatcgcctg gtgcccgaaa tgatttccaa gatcgaaacc
     3901 cccgacgacc gcaccatcgt gattcactgg aacgatcttt acaacgaagc ctatgcgatc
     3961 cagtacaccc atgtgcgcgc cttcccgcgc cacctgctgc tcgatgcttt tcaaggcggc
     4021 gacatgaagg cttttgccaa tttgccgtat tggaacaaaa actttgtcgg cgccggtgcg
     4081 ttcaaagtcg tcgagtggga tcaaggcagt cgcgtggaat tggaagcgtt caacgatttc
     4141 gcgctcggtc ggccaaagat cgaccgcatc acctacaaga ccgttgaaga tagcaatacc
     4201 aatctcgcgg cggtcatggc cggcgaggtc gatgtgtgca tgcgcagcac gatttctttc
     4261 gacggcgcga tgatcctgcg cgatcaatgg gaaaagcaag gcaagggaaa agtgttgatc
     4321 agtccggcgt cgtggagctg gttgagcctc agccgcgaca atccttggtt cagcgacgtg
     4381 agagtgcggc gcgctttgct tcatgccatc gaccgcgatg ccatggtgca aaacctgttc
     4441 aaaggcgaga aagtcatatc ccatttaccg ttgtcgcgcg ctcgcaaggt caacaacaaa
     4501 gcgctggcgg cgtcgacaat ttacaagttc gatgtcgagc gcgccaagaa actcatggcg
     4561 gaggccggtt ggaaggccgg tgccgatggc gtgctggcca acggcaaggg cgaaaagatg
     4621 gagtttgagt tccgtgtcac cgccgagcgg cgcgaccatg aacaggcgca ggcgatcatc
     4681 tccgactatt ggaaaaggct cggcgtgcgc accaatatca agaatatgcc caaccggttg
     4741 ctcaactcgg ccgacaaccg caaccgctgg ccgggcgcgt atatcggttc gcacaacgtc
     4801 accgtggagg agtggcccga acggtttcac tcgaagaata ttccgacggc ggaaaacaag
     4861 tatgcgccgg aaaacgtttc cggttggaac gatccgaaga aggacgccat cctcgacgat
     4921 atcaactcga tcatcacgcc tgaacaatcg gaaaaacttc aaatcgaatt ctgcaagatg
     4981 tttagcgccg cgctgccgca cctgccgcta tttttcacac cggaggttct cgtggtgaaa
     5041 aaggggctga ccggaatcac gccgcgtcag gagagcggcg ggcagaattc gagctcctgg
     5101 aatacccagg cttgggacaa ggtttaagat aaccaccgtc aagggttcaa ggttcaatag
     5161 ttcgaggcgt tcgatacgca attcgtccgg tggttggaac ttggaacttc tgaggcggtt
     5221 cgagtgcccg cgataatccg acgcgactga aaatacttat gttgctctac gttgttcgcc
     5281 ggctgcttca gactgtgccg ctattgctgg tgatatcggc gctgattttt actttactat
     5341 acctgatgcc gggggatccg ctttaccgga tgcttgaagg catacccaat ttgcggccgc
     5401 aggattttga ccggttgcgc aaactctatg gctttgacga tcccgtctat attcaatatg
     5461 gcaagtggct ttggcagttg ctccagctca atcccggcta ctcgcgggag tatggtcagc
     5521 cggtgttcga tattatcctg ccggcgctga aaaatactct ggtgctgacc atcgccgccg
     5581 tggtgctggg taaaatcatc gccatcgccc tcggcatttt ctccgcgatc cggcaatact
     5641 ccatcgccga ttatattctt accgcgacca cgtttgtcgc ttattccgtt ccggcgtttt
     5701 ggctggggct gatgttgatc atcctgttct cggtcaagct cggttggttg ccgacctcgg
     5761 gcatcgtcaa cagtgaactg gagccgggga gttgggcggc gacggtggac tggatgcagc
     5821 atttgatttt gccggtggcg gtgctggcga tctccgagat cattcaagtg cagcgcttca
     5881 tgcggtccag tttactagaa gtattgcgcc aggattattt gaccacggcg cgcgccaaag
     5941 gtttacgcga aagtgtggtc atcggccgcc atgcgctcaa gaacgctctg ataccggtgg
     6001 tgacgatcat cgccgtgacc atgccgcggg tggtcggcgg gtcgaccgtg gtagaaaccg
     6061 tgttcgccta tccgggcatg ggccggctgc tctatacgtc gatcatgggc aatgattatg
     6121 tcgtcgccat gacggtggtg atgatcatcg cggtgactgt ggtgttcttc aatttactgg
     6181 cggacgtgat ttacggctgg ctcgacccgc gcattcgcta tcggagttga agcgaagaga
     6241 tttaaccaca aagagcacag cgcggcaaag ccgcaaccaa agaagaatgg cctcaaggaa
     6301 cgcaaaggga cgcaaaataa aatcccaaga cgaattccgg ttgtaggggc gaatcataat
     6361 tcgccctcgg ggtacggagc ggcgaccgcc tggcgacaaa atttgcgcaa gctgcgaaaa
     6421 ctgtaagcta tagtagtgca aagtgcgcaa aatcattcaa agagaaaatc cgaaggtagg
     6481 agcaggtttt aaacccgcgc gtattggtgg taac
//
LOCUS       DASQKS010000201         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_963457, whole genome shotgun sequence.
ACCESSION   DASQKS010000201 DASQKS010000000
VERSION     DASQKS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963457"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /gene="cysK"
                     /locus_tag="VE170_05240"
     CDS             complement(<1..731)
                     /gene="cysK"
                     /locus_tag="VE170_05240"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYJ14872.1"
                     /translation="MASSNGAQSILDLIGFTPVVKLRKLPGKDDAEVWAKMEAFNPGG
                     SVKDRICLSMIETAERQGKIKPGATIVESTSGNTGIGLALIAAVKGYKLILTMPDTMS
                     EERRSLLSAYGAHLVLTPDTKGMGGAIHKAEELLQENGDFYMPQQFNNPANPEIHRKT
                     TAVELLKQFKRIDAFVAGVGTGGTITGVGEVFKEKMKGVKICAVEPASSPVISGGEPG
                     YHKIQGIGAGFIPAVLNMDILDEVIT"
     gene            813..1832
                     /locus_tag="VE170_05245"
     CDS             813..1832
                     /locus_tag="VE170_05245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYJ14873.1"
                     /translation="MKSLETSRVSMHPPRVCVLFPGALGDFICFLPSLYELTKSYTVD
                     LLARSDFALIAPQGVRVLSLERLEVRQLFIGGGAGDERVRQFFTAYAAVYSWFASQQP
                     VFVDQLQTVSQGRAQIFPFRPDQLPIHQADYYLSCLNSEPRQDFAPLVTLRTKARLWC
                     DAFCQRVGLAHQPLLIVAPGSGSREKNWPEARFLQVVHRWRDRLGGKAVVVVGPVEME
                     RRGFEELASACVVASGLDLGQLAALLARGDLYIGNDSGVSHLAAALGCRTVALFGPSD
                     DRQWAPRGPRVTTLRRMLDDSPCDGTPLHFFPDRADLADLTAAEVIHALSKLPEVAAR
                     WQRSP"
     gene            1949..3022
                     /locus_tag="VE170_05250"
     CDS             1949..3022
                     /locus_tag="VE170_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14874.1"
                     /translation="MERHVLRKLSAVALVMALMAALPAAAQETAGALLAKLAKLSPEA
                     RQKALVDGARAEKEVTFYSSLQPPQVEPFTRAFQKRYPFLKVNAARISGSKVVVRIQS
                     EMNAGKNLVDVINVSGEEAAALKKVGSLDPYFSPQREFYPPSAKDKDGYFTAMSFLTM
                     VLGYNSNLVKRADVPKSYDDLLQPKWKGQMYLDNEAYDWFAVLLKHFGRDKGLQYMRA
                     LAKQEPTMMRGRTAQAQLLVAGERAIGIVQSGNTMLDYKAKGAPVDMSILDPYLALSN
                     SILLAKHALHPHAAALLIDWSLSEEGQNMVTSFGRVVLRKGVKPRFPELMEKELFVAD
                     GEFLAPVLEESAREFRQIFLSGR"
     gene            3226..>3478
                     /locus_tag="VE170_05255"
     CDS             3226..>3478
                     /locus_tag="VE170_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14875.1"
                     /translation="MEVKSIKETHTELMMHSVKFAKDEQGRRKGQEVLFKWPDIEKQL
                     GEHKHIYVVDARLGSNNHTHRLWYDVIPPHTEEGQEWRGL"
BASE COUNT          689 a          977 c         1025 g          787 t
ORIGIN      
        1 gtgatcactt cgtcgagaat atccatgttc aataccgcgg gaatgaaacc ggcgccgatg
       61 ccttgaatct tgtgatagcc cggttcgccg ccggaaatta ccggcgagga cgccggttcg
      121 acggcgcaga ttttgacacc cttcattttt tctttaaaca cttcgccgac accggtaatc
      181 gtgccgccgg tgccgacacc ggcgacaaag gcgtcgatgc gcttgaactg cttgagcagt
      241 tcaactgcgg tggtcttacg gtggatttcc ggattggctg gattgttaaa ttgctgcggc
      301 atgtaaaagt cgccgttttc ctgaagcagc tcctcggcct tgtgaatcgc cccacccatg
      361 cccttggtgt cgggggtaag aaccaaatgc gcgccataag ccgatagcag actgcggcgt
      421 tcctcactca tggtgtcggg catggtcagg atcaacttgt aacccttgac cgcggcaatc
      481 aaagccagac cgattccggt attgccgctg gtagactcga cgatggtcgc gccgggcttg
      541 atcttgcctt gacgctcggc ggtttcgatc atgctcaggc agatgcgatc cttgaccgat
      601 ccgccgggat tgaaggcttc cattttggcc cacacctcgg cgtcgtcttt ccccggaagt
      661 tttctgagtt taacgaccgg ggtgaagccg atcagatcta agatcgactg cgcaccattg
      721 gagctagcca cggattgtct cgactttcat gaaagggagc gaatttagat ttgctactgg
      781 taaaatgcta tattcttggc cggttaatgt caatgaaatc actcgaaacc tcacgcgtat
      841 cgatgcaccc gccgcgagtt tgtgtgctct ttcccggcgc tctgggtgac ttcatctgtt
      901 tcttgccctc gctgtacgag cttactaaat catataccgt cgatcttttg gcccgcagtg
      961 actttgccct catcgcgccg cagggcgtgc gcgtgctttc tttggaacga cttgaagttc
     1021 gccaattatt catcggcggc ggcgctggcg atgaacgcgt gaggcaattc tttacggcct
     1081 atgctgcggt ttattcatgg ttcgccagtc agcagccggt gttcgtcgac caactccaaa
     1141 ctgtatccca gggtcgcgcg cagatttttc cctttcgtcc ggaccagctt cctatccacc
     1201 aagcggatta ttacctttct tgcctcaaca gtgagccgcg acaggatttt gcaccgttag
     1261 taaccctgcg taccaaggcg cggctttggt gcgatgcctt ctgtcagcgc gttgggttgg
     1321 ctcaccaacc attgttgatc gtcgctccag gcagcggctc ccgggagaag aattggcctg
     1381 aagcgcgttt tctccaggtc gtacatcggt ggcgcgatcg gcttggcggc aaggccgtgg
     1441 tggtggttgg ccccgttgaa atggaacggc gcggctttga ggaactggcg tccgcttgcg
     1501 tcgtcgccag cggtctcgac ctcgggcagc tggcggcgtt acttgcgcgc ggcgatcttt
     1561 atatcggcaa cgatagcggc gtgtcccatc tcgcagccgc gcttggttgc cgcacggtgg
     1621 cgctcttcgg tccttcggat gatcggcagt gggcgccgcg cgggccgcgg gtaacgacct
     1681 tgcgccggat gctcgacgat tccccctgtg acggcacgcc attgcacttc tttccagacc
     1741 gcgcagatct agccgatctc actgccgcgg aagtgattca tgcgctgagc aagctgccgg
     1801 aagtcgcagc gcgctggcaa cgatcgcctt gacaggttgc gcggccgcgg catagaagtg
     1861 cgtcatgagt cggctcgggg gcctgcaatg tcgcgattta tctttgcccc aaaaaaactc
     1921 gccgtttgca gccgaaagga gtttggtcat ggaaaggcat gtcttgagga agctctcggc
     1981 agttgcgctt gtcatggcgc tcatggcagc gctgccggcg gcggcccagg aaaccgccgg
     2041 agcgctgctg gccaagctcg ccaagctctc tccagaagcc aggcagaagg cgctcgtcga
     2101 cggcgcgcga gcggagaagg aagtgacttt ttacagttcg ctgcagccgc cccaggttga
     2161 gccgttcacg cgggcgtttc aaaagcgcta tccattcctc aaggtgaacg ccgcgcgcat
     2221 ttccggcagc aaggtggtcg tgcgcataca atccgagatg aacgccggca aaaatttagt
     2281 cgatgtcatc aatgtttcgg gggaagaagc cgcagcgctg aagaaagtcg ggagcctgga
     2341 cccgtacttt tcgccccagc gggagttcta cccgccctcg gccaaagaca aagacggtta
     2401 tttcacggcg atgtccttcc tcaccatggt gttgggctac aattccaact tggtcaagcg
     2461 cgccgacgtg ccgaaaagtt acgatgacct gttgcagccc aagtggaaag ggcaaatgta
     2521 cctcgacaac gaggcctatg actggttcgc ggtactgctc aagcattttg gccgggacaa
     2581 agggctgcag tatatgcgcg cgttggccaa acaggaaccg acgatgatgc gcggccgcac
     2641 cgcacaggcg caactcctag tcgccggcga acgggccatc ggcatcgtgc aatccggcaa
     2701 taccatgttg gattataaag ctaagggcgc gccggtggac atgtcgatct tggatcccta
     2761 tctcgccctg tccaattcga ttctgctcgc caagcatgcg cttcatcccc acgccgcggc
     2821 gttgttgatc gattggtcgc tgtcggagga aggccagaat atggtcacaa gctttggccg
     2881 cgtggtattg cgcaaagggg taaaaccaag atttcccgag ctgatggaaa aagagctgtt
     2941 cgttgccgac ggggaatttc tcgcgccagt gcttgaggaa agcgcccgcg agttccggca
     3001 gatttttctg tccgggcggt aaagcgtgct tcaccgccgg ctgttccctt aacttcaccg
     3061 tcgccactag aatttcgtgg ccatcgattc tccgcgtgat cagtgccgcc accgtgagcg
     3121 ttgctggatt cttggcatgg tttaaccatt gttatacagt cagccgcgtc ggtggcgggt
     3181 tcgagttacc ggcagcgagc gaacaattgg agtctggagg attggatgga agtaaagtcg
     3241 attaaagaaa cccataccga actgatgatg catagcgtca agttcgccaa ggacgagcaa
     3301 ggtcggcgca aggggcagga ggttctcttc aagtggcccg atatcgaaaa gcagttgggc
     3361 gagcacaagc atatttacgt cgtcgacgct cggttgggct cgaacaatca cacgcaccgg
     3421 ctgtggtacg atgtcattcc gccgcacacc gaagaaggcc aagagtggcg cggcctcg
//
LOCUS       DASQKS010000202         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1783517, whole genome shotgun sequence.
ACCESSION   DASQKS010000202 DASQKS010000000
VERSION     DASQKS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783517"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VE170_05260"
     CDS             complement(<1..1006)
                     /locus_tag="VE170_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYJ14876.1"
                     /translation="MRRSVVPSMRISEIHRYISNRLDEKAIVGRTIAGIIGDSPSHYS
                     KSPGLWNAAFRDLGMNAVYLPFDVTEGRLRELLRVLRDSEQFIGINVTVPHKVRVIDF
                     LDDLDAGAARIHAVNTVIRNANGRLIGFNTDGAGFIDSLLLPGPDAPAGFMQSLAGID
                     ALLLGAGGSARAVAFHLSEHLGKGKLIIANRTVAHAQSLANEINQRGHPALAIDEEGI
                     ANWAPKAGVIINSTTKGQGGLRKLHDGMATMLAPYSALAPADPPILAEDLGERFEAQW
                     HEAARSDIQANNRRSEALAAAIPQATRFYDLIYHPEETVFLRHGRVSGHRAMNGKDMI
                     V"
     gene            complement(1066..2499)
                     /locus_tag="VE170_05265"
     CDS             complement(1066..2499)
                     /locus_tag="VE170_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HYJ14877.1"
                     /translation="MSSDFTLDDLERWNERIVELVQRFGLDPYDQEFEVCDHEDMLSY
                     MVYSGMPSHYPHWSYGKSFEKLKTLYDYGVSGLPYEMVINSNPSIAYLMRDNTLALQV
                     LTIAHVYGHNDFFKNNFTFKSTRAEYTIENFKSHANRVRHYVEDPSIGLERVEAVMDA
                     AHALSLQCRRNLAVKKPTAAEERQRMIEEAKVPDDPFRAVHRRRDTAEPDLQKVPLYP
                     DQDLLLFIRDHNPLLAEWEKDLLTIVHEEAQYFIPQIETKIMNEGWASFWHKRILETL
                     DLPQGLHLEFIVRHAQVLRPAPGSINPYHVGMKIWEDIEKRWKNPSAAEEKEFGPRKK
                     SANDKLFEVREVERDSSFLRRYLTEDLIRELNLFEYKARGNEQVVSHVADEESWQHIK
                     DTLIRNVGTGTIPVIKVVESDYQNNRTLLLQHDHDGRDLQLEYAEKTLQYVQQLWGRD
                     VAMETVLDNHRALLTFSGNKLAISKPA"
     gene            complement(2509..>3476)
                     /locus_tag="VE170_05270"
     CDS             complement(2509..>3476)
                     /locus_tag="VE170_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HYJ14878.1"
                     /translation="GGDGEPKPGDVVGQGDDGKAGQQAGNRPGIDYYETDVSLDELID
                     LMFEDLELPDLERKRLRKVELERLFKQKGFRRKGIRVRLDKKRTAKSRIKRKKATQRN
                     QAASAQPQRGAAHVQRFPFHKDDLRYHHTVTDTREESNAVVLCIMDTSGSMDTMKKYL
                     ARSFFFLLYQFISTRYQNVEIVFIAHHTEAQEVTENEFFHKGESGGTMISSGYNKALE
                     IIEQRYHPALWNVYVFHCSDGDNFESDNAATLKAAQELAQTANLFGYGEIKPLGTGYY
                     GSSLISFFAQIQESNFLTVQIQKKEDIWSSFKTFLTKDRAREDAA"
BASE COUNT          682 a          916 c         1047 g          831 t
ORIGIN      
        1 ggacgatcat gtccttgccg ttcatcgcgc gatgtccgga gacgcggcca tggcgcagaa
       61 agacggtttc ttcgggatga taaatcagat cgtagaagcg ggtcgcttgg gggatcgctg
      121 cggccagagc ttccgatcta cgattgttgg cttgaatatc gctgcgcgcc gcctcgtgcc
      181 attgcgcctc gaagcgctcg ccgaggtctt cggcgagtat cgggggatcg gccggagcga
      241 gggcggagta gggtgcgagc atagtggcca tgccgtcgtg cagtttgcgt aggccgccct
      301 ggcctttggt cgtgctgttg ataatcactc cggccttggg tgcccaattg gcaatgccct
      361 cttcatcaat ggcaagcgct gggtgcccgc gttggttaat ttcgttggca agggactggg
      421 catgggcgac ggttcgattg gcgataatca gttttccctt gccaagatgc tcgctcaagt
      481 gaaacgccac ggcacgcgcc gagccgccgg cgccgagcag cagcgcatcg atgccggcta
      541 gcgactgcat gaaaccggcc ggcgcatccg gacccggcag aagtagactg tcaataaatc
      601 ctgcgccgtc ggtgttgaag ccaattagcc ggccattggc gtttcggatc acggtattga
      661 cggcgtgaat ccgcgccgcg ccggcatcga ggtcgtccag gaaatcgatt acccgcactt
      721 tgtgcggcac agtgacgttg atgccgatga actgttccga atcgcgcaaa acccgcagca
      781 gctcgcgaag ccggccctca gtcacgtcga aaggcagata gacggcgttc attcctaaat
      841 cgcggaacgc cgcgttccac aggccggggc ttttcgaata gtgcgaagga ctgtcgccga
      901 taatgccggc aatggttctg ccgacgatgg ctttttcatc gagacggttg gagatatatc
      961 ggtgaatttc actgatgcgc attgacggta ccactgatct acgcatgcgc ctaaagtagt
     1021 cgcatgcaga gtcgggtttg gcaaccgaca gcggccggcg ggcgcctagg ccggtttgct
     1081 gatggcgagc ttgttgccgg agaaggtcag cagagcgcgg tgattgtcga gcacggtttc
     1141 catggcgacg tcccggcccc aaagctgttg gacatattgc agggttttct cggcgtactc
     1201 cagttggaga tcgcggccgt catggtcgtg ctgaagcagc agcgtgcgat tattctgata
     1261 gtccgattcg acgaccttga tgaccgggat agtgccggtg ccgacgttac ggatcaaggt
     1321 gtctttaata tgctgccagc tctcttcgtc ggcgacatgg ctgactactt gttcattgcc
     1381 gcgggctttg tattcgaaca aattcaattc gcgaatcaga tcctcggtga gatagcggcg
     1441 cagaaatgag ctgtcgcgct cgacctcgcg cacttcgaaa agtttgtcgt tggcgctttt
     1501 cttcctcggg ccgaactcct tttcctcggc ggcgctggga tttttccagc gcttctcgat
     1561 gtcttcccaa atcttcatgc cgacatggta gggattgatg ctaccgggcg caggacgcag
     1621 cacctgggcg tggcgtacga tgaattccaa atgcaacccc tgcggtaaat ccagggtttc
     1681 gaggatgcgc ttatgccaaa aactcgccca gccttcgttc atgatcttgg tttcgatttg
     1741 cggaataaaa tactgggctt cttcatggac gatggtcaac aaatcttttt cccattcggc
     1801 gagcagtgga ttgtgatcgc ggataaacaa cagcagatct tggtcggggt agagcgggac
     1861 tttttgcaga tcgggttcgg cggtatcgcg ccggcggtgt acggcgcgaa acgggtcgtc
     1921 cggcactttg gcttcttcga tcattcgttg ccgttcttcg gcggcggtgg gcttttttac
     1981 cgcgagattg cgccggcatt gcagcgacaa cgcgtgggcg gcgtccatga ccgcctcgac
     2041 ccgctccagg ccgatgctcg ggtcctcgac atagtggcgc acccggttgg cgtggctctt
     2101 gaagttttcg atggtgtatt cggcgcgggt cgacttaaag gtgaaattgt ttttgaaaaa
     2161 atcgttatgg ccgtaaacat gggcgatggt cagcacctgc agtgccagcg tgttgtcgcg
     2221 catcaaatag gcgatcgacg gattggaatt gatgaccatt tcgtagggca gaccgctgac
     2281 gccgtagtcg tagagcgtct tgagcttctc aaaactcttg ccgtatgacc agtgcggata
     2341 atgggaaggc atgcccgaat agaccatgta ggataacatg tcttcgtgat cgcaaacctc
     2401 gaactcttga tcgtaggggt cgaggccgaa ccgctggacc agttcgacaa tgcgctcgtt
     2461 ccaacgctcc agatcgtcga gggtgaaatc ggaactcatg gcatcgcctc aggcggcatc
     2521 ttcgcgggcg cggtccttgg tgagaaatgt tttgaacgag ctccagatat cttctttttt
     2581 ttgaatctgc accgtgagaa aattcgattc ttggatctgc gcaaaaaaac tgatcaagct
     2641 gctgccgtag tagcccgtgc ccagaggttt gatctcgcca tatccgaaca gattggcggt
     2701 ctgggcgagt tcttgggcgg ctttaagtgt cgctgcattg tccgactcga aattgtcgcc
     2761 gtcggagcag tgaaacacgt aaacgttcca gagcgcggga tggtagcgct gctcgatgat
     2821 ctccagcgct ttattgtagc cggaggaaat catcgtgccg ccggattcac ccttatgaaa
     2881 aaactcgttt tcggtgacct cctgggcctc ggtatggtgc gcgatgaaga cgatctcgac
     2941 gttctgatag cgtgtcgaga taaattgata gagtagaaag aagaaactgc gcgccagata
     3001 tttcttcatc gtgtccatgg accccgacgt gtccatgatg cataggacca ccgcgttgga
     3061 ttcctcccgg gtgtcggtaa ccgtatgatg atagcgcagg tcgtctttat gaaaggggaa
     3121 gcgctgcaca tgggctgcgc cgcgctgcgg ctgagcggaa gcagcctggt tgcgttgagt
     3181 cgccttcttg cgtttaatcc gcgatttggc cgtgcgtttt ttatcgagac gaacgcggat
     3241 gcctttgcgg cggaaacctt tctgcttgaa caaccgctca agctcgactt tgcgcaagcg
     3301 cttacgttcc aagtcgggga gttcgagatc ctcgaacatc agatcaatca gttcatcgag
     3361 actgacatcg gtctcataat agtcgatgcc ggggcggtta cccgcctgtt gtccggcttt
     3421 gccgtcgtcg ccttggccaa ccacatctcc cggcttgggt tcgccgtcgc cgccct
//
LOCUS       DASQKS010000203         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_105939, whole genome shotgun sequence.
ACCESSION   DASQKS010000203 DASQKS010000000
VERSION     DASQKS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105939"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..748
                     /locus_tag="VE170_05275"
     CDS             335..748
                     /locus_tag="VE170_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14879.1"
                     /translation="MGGFKITALDHIVLNVSDIDRALTFYTEVLGLEGERVELFRAKK
                     VGFPSVRINDATIIDLFPRQTVDSQPVLEKVDGNLNHVCLVVDADAFAAVIEELKKNR
                     IALRAGPVSRWGARGQATSVYFLDPDGNEIEIRCY"
     gene            complement(825..2138)
                     /locus_tag="VE170_05280"
     CDS             complement(825..2138)
                     /locus_tag="VE170_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYJ14880.1"
                     /translation="MIVDNFWLEAILILALIFANGFFAASEIAMIAMRKSRIDALVEK
                     GIPCAAALARLKADPDRFLATVQIGVTVVSSFASAVGGAAAIGYLKPAIQALPFTLTT
                     RWADAIALTIVVLPIAYFSLVLGELVPKSLALRFSEPIACFVARPIELLARGSAIFVR
                     LLTASSNLVLKLFGGKDAGSVTFISVDEVKSLIREGAARGIFNETEKELIHSVFEFTD
                     TPVKAVMKPRTEIHAIEAHTPLAEVAKDFVESGFSRVPVYEGDLDKVIGILYNKDVFK
                     ALQEHSDFQVRDHLHPAFFVPSTLPISELLKQLQRRRLGMALVVNEFGEIEGLATLED
                     LIEEIVGEIRDEYDREERGPVERLPDGSMVIAGSALLKDLKIDFDLPFEESPDYHTLA
                     GLMLARLKRIPRGGEWIDELGYKLTIVDMEGRRIVKIKVEKSPAS"
     gene            2363..>3034
                     /locus_tag="VE170_05285"
     CDS             2363..>3034
                     /locus_tag="VE170_05285"
                     /inference="COORDINATES: protein motif:HMM:NF025843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYJ14881.1"
                     /translation="MADKQNELTSIQGTISRITYQHPESHYTVARLDEDGGPAATVVG
                     VLFPVSEGEEIKVTGVWKRHARYGLQFQVDHWTKVDPATLEGIEKYLGSGMIKGIGPT
                     YAKRLVGAFGLDTLRVLSEEPLRVLDVDGIGEVRARRIMEAWQAQRGMQDVMVFLQGH
                     GVGASLALRIYRVFGADTVAQIRDNPYALAQQVHGIGFLLADRLAGHIGIRGDFPLRV
                     QAGVLH"
BASE COUNT          648 a          809 c          923 g          654 t
ORIGIN      
        1 aacgcggcaa gaatttgcaa cccgtctacg aagagctaaa gcggcgaaaa attttgctgc
       61 gctggttcga cgcgcccggt ttgcgcgact gcctgcgtat caccgtggga acacccaagg
      121 aagttcaggc gttgttaaaa gagctggcgg cgatgccagc ttattccgga tgattcggtg
      181 tcgtgcgccc ctttggtatc tcgaagtgat cctgtgactt gtttgtcgaa gttttaccgt
      241 cggtagcgat agcgtgtagc ggggtggtat atgaccttgc gattacacat taccggttga
      301 ttgaaatatt ggctcgatcg aaaggaatta cgccatgggc ggtttcaaga ttactgcgct
      361 cgatcatatc gtgctcaatg tcagcgacat cgatagggcg ctgacattct acactgaagt
      421 gctcggcttg gaaggcgaac gggtcgaact atttcgcgcg aaaaaagtcg ggtttccatc
      481 ggtgcgcatc aacgacgcta cgattatcga tctcttcccg cgccagactg tcgacagcca
      541 gccggtttta gaaaaggtcg atggcaacct caatcatgta tgcctggtcg tcgatgctga
      601 tgcgtttgcc gcggtaatcg aagagttgaa aaaaaaccgg attgcccttc gcgccggtcc
      661 tgtgtcccgc tggggcgcgc gcgggcaggc aacgtcggta tattttctcg atcccgatgg
      721 taatgagatc gagattcgct gctattgaag aaccggagta acggagtaat ggagtggcgg
      781 gggcccgata ctccaggact ccaacactcc aaagccctat gttgctacga cgctgggctc
      841 ttctcgactt tgattttgac gatgcgccgg ccttccatgt cgacgatggt gagtttgtag
      901 ccgagttcat cgatccattc gccgccgcgc gggattcttt tcaatcgcgc cagcattaag
      961 ccggccaggg tgtgatagtc cggcgattct tcgaacggca ggtcaaaatc gattttaaga
     1021 tccttgagca gagccgagcc ggcgatcacc atcgacccgt ccggcaggcg ctccacgggg
     1081 ccgcgttctt cgcgatcgta ttcgtcacgg atctcgccga cgatctcctc gatcaagtct
     1141 tccaatgtgg cgaggccttc gatttcgccg aattcgttga ctaccaaggc catgcccaga
     1201 cggcgccgtt gcaactgttt caacagttcg ctgattggca gggtgctggg tacaaagaac
     1261 gccggatgca aatgatcacg cacctgaaag tcgctgtgct cttgcaaggc tttgaacaca
     1321 tctttgttgt agagaatgcc gataacttta tcgagatcgc cttcgtagac cggcacgcgg
     1381 gaaaagccgc tttcgacgaa atccttcgcc acttcggcca gcggtgtgtg cgcttcgatg
     1441 gcatggatct cggtgcgcgg tttcatgacc gctttgaccg gcgtatcggt aaactcaaaa
     1501 acgctatgga tcaattcctt ctcggtctcg ttgaaaatgc ctctggcggc accttcgcga
     1561 atcaatgatt ttacttcgtc gaccgagatg aaagtgacgc tgccggcatc cttgccgcca
     1621 aaaagtttga gtaccagatt gctcgacgcg gttaagagcc tgacgaagat cgcgctgccc
     1681 cgcgccagta attcaatcgg gcgggcaacg aagcaggcga tgggctcgga gaatcgcaaa
     1741 gccaaggact ttggcaccag ttcgccgaga accagcgaaa aataagcgat cggcaatacc
     1801 acaatggtca gcgcgatggc atccgcccag cgggtggtca gagtaaaggg caatgcctgg
     1861 atcgcgggtt tgagatagcc gatggcggcg gcgccgccga cggcggaggc gaaggaactg
     1921 accacggtga cgccgatctg tacggtggcg aggaagcgat cgggatcggc ctttagccgg
     1981 gccagcgccg cggcgcaggg aattcctttt tcgaccaggg cgtcgatgcg gcttttgcgc
     2041 atggcaatca tggcgatctc ggaagcggca aagaagccat tggcgaaaat caaagcgagg
     2101 atcaaaatcg cttcaagcca aaaattatcg acaatcatcg gtgccgtctc ctgaattggg
     2161 atttgggcgt agatgcagcg agcgaagcgt aagacagggc ggtggtgcta gcaagtcgac
     2221 cggggttgcc ggggccgcgg gcgtgggctg cgagtgggca tgggggttag caggaattct
     2281 agcgggccac cggcaaaagg caagggctgc gatttacgct ttgaccgctt tcaagtagga
     2341 tcggccccct agggagtatt ttttggcgga taaacagaac gaactcacgt ccatccaggg
     2401 taccatcagc cggatcacct atcagcatcc agagagccac tacacggtgg cgcgcctgga
     2461 cgaagacggc ggaccggccg ccaccgtggt cggcgtgctg tttccagtgt cggaaggcga
     2521 ggagatcaaa gttaccggcg tgtggaagcg ccacgctcgt tacggcctgc aatttcaggt
     2581 cgaccattgg accaaggtcg atccggcgac ccttgaagga atcgaaaagt atctcggctc
     2641 gggcatgatc aaaggcatcg ggccgaccta cgccaagcgg ctggtcggtg ccttcggttt
     2701 ggatactctg cgggtattat ccgaagaacc gctgcgtgtt ctcgatgtcg acggcatcgg
     2761 cgaagtgcgc gcgcgccgga tcatggaagc ctggcaggct cagcgcggca tgcaggatgt
     2821 tatggtattt cttcaaggcc acggcgtggg cgcgtctctg gcgctgcgca tttaccgagt
     2881 gtttggcgcc gacacggtcg ctcagattag agacaatccc tacgcgctcg cgcagcaggt
     2941 ccatggcatc ggctttctgc tcgccgaccg gctcgccggc catatcggca tccgcggcga
     3001 ttttccgctg cgggtgcaag ccggtgtcct gcat
//
LOCUS       DASQKS010000204         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5566459, whole genome shotgun sequence.
ACCESSION   DASQKS010000204 DASQKS010000000
VERSION     DASQKS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5566459"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VE170_05290"
     CDS             <1..253
                     /locus_tag="VE170_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14882.1"
                     /translation="NAPRSATANVEIDSLLWRLDRSVFHDIFSHHPEMAAKICYRLVT
                     IFIERLRVTNEQTRDAIRWGLEATGYASGNQSLFGRKST"
     gene            262..1485
                     /locus_tag="VE170_05295"
     CDS             262..1485
                     /locus_tag="VE170_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14883.1"
                     /translation="MAEPAKLQLLTRKPGEAGFQLHEIHSSQAHLGYRPRRIADEFWA
                     ITAQPQSAGGGRVYVLNNLQRDRYLLLDSKEFYLWSLFDGSHSLEEIARSFHHQFGAF
                     DYAMIRLLLAKLYQAGLLVERGNLALQQSLVRSQTKFWAMGFSSAAKYWRRIAFIVPN
                     ADRLCSTLYRYGGYLLFNPVTFGLTLALALLAGIDAWRLSPQTKQFAQLLAATPWFSG
                     ALMLAALLAVSMMHVAVHALACTSYSRRVREIGFFLLQGVLPTFYADVTDIFMSTRRA
                     RVIVDLAGPLVELSWGSVAFLLAYHSPPGIGQAFFFGIGVVLWEGALINLYPFSFLEL
                     DGYNIVADLLAMPTLRQQALALAPQLPQRLLHPRSLTKAEWIQIAYLLLCSVSVLIYL
                     IAHLDFVRGLLPSWQ"
     gene            complement(1596..2603)
                     /locus_tag="VE170_05300"
     CDS             complement(1596..2603)
                     /locus_tag="VE170_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14884.1"
                     /translation="MPATFVAAIFFIYATCAGFTCAVRAAESIPLERINFGFSAAGSV
                     ASAPLWMAQDSGAFKKYGLDVKLIFLAGGLSPVAVMAGEVPFALMSAGVMVPAALRGA
                     DLVMVAALSNYLNQVLAVAPEFTDVKQLKGKRIAIQRLGDLTHIAAREALKNLGLNES
                     DMQMQQIGGGPARFIALQTGNVQAAILTPPYSTRARKLGYRVPVNLYDLKIPFIGTGV
                     VTTRQLVAARRPLVLNLLKAIAEGIRFYKIERDLPIKIAERHLPGAAREEISDALDHY
                     NRDLDERPFPRAEGFKIALELTAQQTPLAKGVDAERFIDASLLRELERGGFFAVPSGG
                     K"
     gene            complement(2612..3589)
                     /locus_tag="VE170_05305"
     CDS             complement(2612..3589)
                     /locus_tag="VE170_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14885.1"
                     /translation="MQKGIAVIVFLSALVVAGSARAASEPVRLKIIYSSFTGAYTPLW
                     LAVEEKLGRKYGLEFEAVYAGRARPHQLLLSGDAQYVVSTGTGVVSSYAVGTKDLVII
                     ASFVNATGSSIFSKPQIKTAKDLRGKTVASGRPGAVSDILLHYILKRKIGLEPGRDVK
                     IVNVGDTPAILPALEKGVVDGAMLTTPSRLVAKRAGFRELVDFDELGVQYPYVGISTL
                     KVNVKKNPDVTLRLVRALTDAIQVFKTSRERSMSAMKKYLRGASDEMLDETYGYFTTR
                     MPRFPYPSVEAIKTALEMMGDQFPQAASVDPQEVVDMSFVKQVESSAVK"
BASE COUNT          719 a         1129 c          993 g          771 t
ORIGIN      
        1 caacgcgccg cgttcggcca ccgccaacgt cgaaatcgac tcgctcctat ggcgtctcga
       61 ccgcagtgtc tttcacgata ttttttccca tcacccggag atggcggcga aaatttgcta
      121 tcggctggtg acgattttca ttgaacggtt gcgagtcacc aacgagcaga cccgcgacgc
      181 catccgctgg ggtttggaag ccaccggcta tgcctccggc aatcaatccc tgttcggccg
      241 caagtcgacg taagccggat catggccgaa ccggcgaaac tccagctgct cacgcgcaaa
      301 cccggcgaag cgggctttca gctccacgag atccacagca gccaagccca tctcggctac
      361 cggccacggc gtatcgccga cgagttctgg gcgatcaccg cgcaacccca gagcgccggc
      421 ggcggccgcg tctatgtgct gaacaacctc cagcgcgacc gctacctgct gctcgacagt
      481 aaggaatttt atctgtggag cttgttcgac ggtagccatt ccctcgaaga gatcgcccgc
      541 tcttttcatc atcaattcgg cgccttcgac tacgcgatga tccgcctgct gttggcgaaa
      601 ctgtatcaag ccgggctact cgtcgaacgg ggaaatttag cgctgcaaca aagtctagtc
      661 cgcagccaga ccaaattctg ggcgatggga tttagttccg cagccaaata ctggcgcaga
      721 atagccttca tcgtgcccaa cgccgaccgt ctctgttcaa cgttataccg atatggaggt
      781 tatcttttgt tcaatccggt tacattcgga ttgaccctgg cgctggcgct gctcgccggc
      841 atagacgcct ggcgcttgag cccccagacc aaacagttcg ctcaactgct tgccgctacc
      901 ccatggttca gcggcgccct catgctggcc gctctattgg cagtatcgat gatgcatgtg
      961 gccgtgcacg cgctggcgtg cacatcctac agccggcgcg tccgcgagat tggcttcttt
     1021 ctactgcaag gagtgctgcc gacattttac gccgacgtta ccgatatctt catgtcgaca
     1081 cggcgggcgc gggtgatcgt cgatctcgcc ggcccgttgg tcgaactgtc ttggggcagc
     1141 gtggcctttc tcttggccta tcactccccg cccggcatcg gccaggcgtt cttcttcggc
     1201 atcggcgtgg tgctctggga aggcgctctg attaatctct atccctttag tttcctcgaa
     1261 ttggacggct acaacatcgt cgccgatctg ttggcgatgc cgaccctgcg ccagcaagcg
     1321 ttggcgctgg caccgcaact gccgcagcga ctgttgcacc cacgcagttt gacaaaagcg
     1381 gaatggattc aaatcgccta tttgctgctc tgctcggtct ccgtattgat ttacctgatc
     1441 gcccacctcg actttgtccg cggcttgcta ccgagttggc agtgaacaac tgtccacctc
     1501 ggggcgaaga aaaagaaggc ggactccttc gcattcattc ttgtgaaatc cgcaatccag
     1561 gttcctttgt aatcgaccgc cgttcgctaa ctcgcttatt tcccgccgct gggaacggcg
     1621 aaaaaaccgc cacgctctaa ctcacgcaac aacgacgcgt cgatgaatcg ttcggcatcg
     1681 acgcctttgg caagtggcgt ttgctgcgcg gtaagctcca gggcgatttt gaagccctcg
     1741 gcgcgaggga acggccgctc atccaggtca cgattgtaat gatccagagc atcggatatc
     1801 tcttcccggg ccgctccggg taaatgacgt tcggcaatct tgatcggtag gtcgcgctca
     1861 attttataaa agcgaatacc ttcggcgatc gccttgagca gattcagcac cagcggccgt
     1921 cgcgccgcga ccaactgcct cgtggtgacc acaccggtgc cgatgaacgg gatcttaaga
     1981 tcgtaaagat tgaccggcac gcggtagccc aatttgcgcg ccctggtgct gtagggcggc
     2041 gtcaatatcg cagcctggac attgcccgtt tgcagcgcga tgaagcgcgc cggcccgccg
     2101 ccgatctgtt gcatctgcat gtcggactca ttgagcccaa gattcttgag cgcttcacgc
     2161 gccgcgatgt gcgtcaggtc gcccaagcgc tgaatggcga tacgtttacc tttcagctgt
     2221 ttcacatcgg tgaattccgg cgccacggcg agcacctggt ttaggtaatt gctcagcgcc
     2281 gcgaccatga ccaggtccgc gccccgcagc gccgccggca ccatgacacc ggcggacatg
     2341 agcgcgaacg gcacttcgcc ggccatcacc gccaccggcg acaagccgcc ggcaagaaaa
     2401 atcagcttga cgtcgaggcc gtattttttg aaggcgccgc tgtcctgggc catccacaat
     2461 ggcgccgacg ccaccgagcc ggcggcgcta aaaccgaaat tgatgcgttc aaggggaatc
     2521 gattcggccg cccgcaccgc gcaagtaaac ccggcacaag ttgcgtagat gaaaaatatt
     2581 gcggcgacga aggttgcggg catgagactt cctactttac tgcggaactc tcgacctgct
     2641 tcacaaatga catatcgacg acctcttgcg gatcgacgct tgcagcttgg gggaattgat
     2701 cccccatcat ctccaacgcc gtcttgatcg cctccaccga cgggtaaggg aagcgcggca
     2761 tgcgcgtggt gaaatagccg taggtctcat cgagcatctc gtcgctggcg ccgcgtaaat
     2821 atttcttcat cgcgctcatg ctgcgctcac ggctggtctt gaacacttga atggcatcgg
     2881 tcagcgcgcg caccaagcgc aaggttacat ccgggttctt tttgacattg accttgagcg
     2941 tcgagatgcc gacgtaggga tactgcacac cgagctcgtc gaagtcgaca agttcgcgga
     3001 agccagcgcg tttcgccacc agtcttgatg gcgttgtcaa catcgcgccg tccaccacgc
     3061 ctttttccag tgccggcaag atcgccggcg tatcgccgac gttgacgatc ttgacgtccc
     3121 gccccggctc cagaccgatc tttcgtttga gaatgtaatg cagcaagata tccgacaccg
     3181 cccccggacg gcccgacgcg acggttttgc cgcgcaggtc tttcgctgtc ttgatctgcg
     3241 gcttggaaaa aatcgacgag ccagtggcgt tgacgaacga tgcgatgata accaaatcct
     3301 tcgtgccaac ggcgtaactc gacaccacgc cggtgccggt tgagacgaca tactgggcat
     3361 cgccgctgag caagagctga tgcggccgcg cccggccggc gtacacggct tcgaattcca
     3421 aaccgtactt gcgccccaac ttctcttcca ccgccagcca gagcggcgtg taggcgccgg
     3481 tgaatgacga ataaataatc ttgaggcgca ccggctcgct ggcggcgcgc gcgctgccgg
     3541 caacgactag cgcactgaga aaaactatga ctgcaatgcc tttttgcatg attccattcc
     3601 gtttctagat tt
//
LOCUS       DASQKS010000205         5733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1785350, whole genome shotgun sequence.
ACCESSION   DASQKS010000205 DASQKS010000000
VERSION     DASQKS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785350"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VE170_05310"
     CDS             <1..225
                     /locus_tag="VE170_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14886.1"
                     /translation="RGVEILAMGVLGGTGSPWGAVAGGVLITLLPELLRGLGDYRPLI
                     NGMILIVIILYSPKGIWDLTGYWHRRAAVD"
     gene            228..1016
                     /locus_tag="VE170_05315"
     CDS             228..1016
                     /locus_tag="VE170_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14887.1"
                     /translation="MLRLSSIAKSFGGLQVLHNISLTVPERGIFGLIGPNGAGKTTVF
                     NLITGLLAPTSGTIEFRGRPLNGLAPHRITRCGIARTFQNIRIFKDMSLRENVLVALG
                     RQQRYGAMRVLWPSARWRAAQRRERAEAEMLLAKVGLADKAAILAGKLPYGEQRRLEI
                     ARALATAPELLLLDEPAAGMNGAEKQQLMEEIVKLEASGLAILIIEHDMRFVMGLCRQ
                     IAVLNFGQLIAEGTAAEIRANPAVVEAYLGEESADAAVPVQGVS"
     gene            1013..1726
                     /locus_tag="VE170_05320"
     CDS             1013..1726
                     /locus_tag="VE170_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ14888.1"
                     /translation="MNGLLQVENLQVRYGAIEAVRGISFTLEPGQIVTLLGANGAGKS
                     TTLLALSGLLPISAGSIQFDGNELRGQAPHQIVDLGLVQVAEGRAILATLTVQENLLL
                     GAYRRHSRAEISDDLKKMLELFPRLRDRFGGAAGNLSGGEQQMLAIARALMARPRVLL
                     LDEPSMGLAPLLVQEIFRTLQQLHRSGLTILLVEQNVRQALKIAEYAYVMENGAIVLS
                     GRSDELLHNDKVIAAYLGG"
     gene            2043..2246
                     /locus_tag="VE170_05325"
     CDS             2043..2246
                     /locus_tag="VE170_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008108598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ14889.1"
                     /translation="MDEILTAGEVAELLQIHPRTVYKLVKQGSIPGRKFGGGWRFSRT
                     DIVAMVSPQGQPPAPRQLHTEER"
     gene            2424..3599
                     /locus_tag="VE170_05330"
     CDS             2424..3599
                     /locus_tag="VE170_05330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ14890.1"
                     /translation="MGGALAGIKVIEAASYVTGPFAAQLLADLGAEVIKVEEPKRGDP
                     FRGWGERNYAATFCSLNRNKKSITLDFRTDEGRDVLLQLLSGADVFLQNFRPGVLDKR
                     GLGYDVIKKLNAKIVYCSISGFGPKGPYRDMPGYDTIGQARSGLLSLLTDPGRPQGMG
                     ISFSDHLTGMYACYGVLGALMERAHSGEGQHVETSLLRASISFVGENAARYFETGHVP
                     RRKHRTTTAGVFAFEDQDGLAFVLHMSSPDKFWLGLFDVVGKPEWAQDPRFNNRKSRV
                     ENYDAINEQLAPIFRAGKRDDWLRRLIAKDVPAAPINTLDEVFADPQVKTYGFPIEVE
                     HPQMGKMQLIGNAVDMSRTPPAIDRPPPVLGEQTEEVLMALGYDSEKIANLRQQGAV"
     gene            3705..4172
                     /locus_tag="VE170_05335"
     CDS             3705..4172
                     /locus_tag="VE170_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HYJ14891.1"
                     /translation="MRHPSLLPLSHDHHHGLALALRCRKQGLGQIKPMGAEGLRERAK
                     EFLEFYRANLVAHFSAEEQVLFPLMRQQVAGCGAMVDQLVQDHDQLRRAMPQLESGTG
                     LAKLIFDLGDLLERHIRTEERELFPLFEAHVDAAQAGLVGAKLQELLNPQGAR"
     gene            complement(4193..4546)
                     /locus_tag="VE170_05340"
     CDS             complement(4193..4546)
                     /locus_tag="VE170_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14892.1"
                     /translation="MAGKAKYLMIASMDVDAEHDAIFNEVYDQEHVPNLLKVPGVLGV
                     SRYKRGTLTMNIGGESKTIEIPNEPTYTAIYELESPDVLTSPAWDKAIEDGRWPGQVR
                     PHTKNRRHVLLKLMD"
     gene            complement(4580..4942)
                     /locus_tag="VE170_05345"
     CDS             complement(4580..4942)
                     /locus_tag="VE170_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14893.1"
                     /translation="MILSKNADVDAIIVKAIEGKPMYGGDLRVKPLITGEQMTFLEIY
                     YTPGVGAPLHVHNHESLAYVVKGKVKMIVGKEEFILGPGDVCRHPKGVPHGVEGIEES
                     IVVEIKSPAPELGSFLGM"
     gene            5107..>5733
                     /locus_tag="VE170_05350"
     CDS             5107..>5733
                     /locus_tag="VE170_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ14894.1"
                     /translation="MLVQALDHFVVPVNDIVEAEDFYVQVFGARIAKRNGLNVKSQSL
                     GPHTFMDIAGKRIGVYLQKEERGQPVAPRGAPTYSFTTTKHGIEEVMDVLANWKIGFE
                     GPQKNSHPFATTTLFFADPAGNNFAVYVPSVDGRNTPPGGGRLTGVGYLELEAPKLED
                     SIKFYQSVLGFELLNRGQEPKTDRNQATMRMASGQYLILTEASFSPKGY"
BASE COUNT         1144 a         1601 c         1736 g         1252 t
ORIGIN      
        1 cgcggcgtcg aaattttggc gatgggcgtc ctcggcggca ccggcagccc ctggggcgcg
       61 gtggccggcg gcgtactgat taccctgctg ccggagttgt tgcgcggcct cggtgattac
      121 cggccgctga tcaatggtat gattttgatc gtcatcattc tttactcgcc gaaggggatt
      181 tgggatctca cgggttattg gcatcggcgc gccgcggtcg actagtcatg ctgcgcttgt
      241 cttccatcgc aaaaagcttt ggcggtttgc aggtgctgca taatatcagc ctgaccgttc
      301 ccgagcgcgg tatctttggc ctgatcggtc ccaacggtgc gggcaagacc acggtgttca
      361 atttgatcac cggcctgctg gcgccgacca gcggcaccat cgaattccgc ggccgaccgt
      421 taaacggatt agcaccgcat cgaattaccc ggtgcggcat cgcccggacc tttcagaata
      481 tccgcatctt caaggacatg tccttgaggg aaaatgtctt ggttgctctc gggcggcagc
      541 aacgctatgg cgccatgcgg gtactctggc cgtcggcgcg ctggcgcgcg gcgcaacggc
      601 gcgaaagggc cgaagcggag atgctgctag ccaaggtcgg tctcgccgac aaagcggcta
      661 tcttggccgg caagcttccc tatggcgaac agcgccgttt ggaaatcgcc agggcgctgg
      721 ccacggcgcc tgaacttttg ctgctcgacg aaccggcggc gggaatgaat ggcgcggaaa
      781 agcagcagct gatggaagag atcgtcaagc tggaagcgag cggcttagcc attttgatca
      841 tcgaacatga catgcgtttt gtcatgggac tgtgccggca gatcgccgtg ctgaatttcg
      901 gtcaactgat tgccgagggc actgccgcgg aaatacgcgc caatcccgcc gtcgtcgaag
      961 cgtatctggg cgaagagagc gccgatgctg cagtgccggt gcaaggtgtg tcatgaacgg
     1021 gttacttcag gttgaaaatt tacaagtgcg ctacggtgcc atcgaagcgg tcagaggcat
     1081 tagtttcacc cttgagcccg gacagatcgt caccttgctc ggcgccaatg gagccggcaa
     1141 gtcgaccacc ttgctggcgc tttcgggcct gctccctatc agcgccggtt cgatccaatt
     1201 cgacggcaat gaactgcgcg gccaggcgcc ccatcagatc gtcgatctcg ggctggtcca
     1261 agtggccgaa gggcgcgcga ttctcgccac gttgacggtc caggagaatt tgctcctcgg
     1321 cgcctatcgg cgccattccc gtgctgagat tagcgacgac ctgaaaaaaa tgctggaact
     1381 gttcccgcgc ctgcgcgacc ggttcggcgg cgccgccggc aatctctcgg gcggcgaaca
     1441 acagatgctc gccatcgccc gggccttgat ggcgcggccg cgcgtgctgc tattggacga
     1501 gccgtcgatg ggtttggcgc cgctcttggt tcaggaaatt tttcggacat tgcagcagct
     1561 ccaccgcagc ggattgacga tcttactggt cgaacagaat gtccgccaag cgctcaagat
     1621 cgccgagtac gcttacgtca tggagaacgg agcgatcgtc ttgtcggggc gcagcgacga
     1681 gctgctgcat aacgacaagg tcattgcggc ctatctcggc ggctaatgtt gtctcgccgc
     1741 cccggcgttc atttcatgac tgtcgggaaa cactgcagta cgcaaacgcg ccagttgttg
     1801 tagtttctgg agtctgaaag gaatccagcg gggttccagc gacgggcgga aaacttacag
     1861 gtttgttccc aattgtgccg aacactgctg ttagtgcgca atatgcttgg tgtgcttttc
     1921 gcggtttgtc cgatagttgc gcggctggcc atttctgtcc cgaaaaaccg gccgggtgtt
     1981 cgtggcatgg tttctgccta actgtctcta taagtttgat tccaattgaa ggaggagatt
     2041 tgatggatga aattttgaca gccggagaag tggctgaact gctacagatc cacccacgga
     2101 cggtctataa gctggtcaag caaggttcga ttcctgggcg taagttcggc ggcggctggc
     2161 gttttagccg caccgatatc gtcgccatgg tttcgcccca agggcaacca ccggcacccc
     2221 gccagttgca caccgaagag cgttagtttc ccagatcgac atttcaccaa gaagccctcc
     2281 cgcatcgagt cgcgccgact acgaacggga gggttttttt attgccgcgc tgcagtgcct
     2341 cagcgcaaac gcccgcgccg gcttgcttcc gttaggccga gcagttagga aatacggatc
     2401 attctgtaca aaggacggct aacatgggtg gggcactagc gggaataaaa gtcatcgaag
     2461 cggcgagtta tgtgaccggg cccttcgccg cgcagttact ggcggatttg ggcgcggagg
     2521 tgatcaaagt cgaagagccc aaacgcggcg atccgtttcg cggctggggt gaacgcaact
     2581 acgcggcgac tttctgcagt ctcaaccgca acaagaaaag cattacattg gacttccgca
     2641 ccgacgaggg gcgcgatgta ctgcttcaat tattaagcgg cgccgatgtg tttctgcaaa
     2701 actttcggcc cggtgtcttg gacaagcgcg gcctcggtta cgacgttatc aaaaagctca
     2761 atgccaagat cgtttactgt tcgatctccg gcttcggtcc caaagggccg taccgcgata
     2821 tgccgggtta cgataccatc ggccaggcgc gtagcggctt attgagcttg ctgaccgatc
     2881 cgggcaggcc ccaaggcatg ggtatatcgt tttccgatca tctcaccggc atgtatgcct
     2941 gctacggcgt gttgggcgcg ctgatggaac gcgcgcacag cggcgagggc cagcatgtcg
     3001 agacttcgtt gttgcgcgcg tcaatatctt tcgtcggtga gaacgcggcg cgctatttcg
     3061 agaccggcca tgtgccgcgg cgcaagcatc gcaccacgac tgccggcgtg tttgctttcg
     3121 aggaccagga cggcctggcg ttcgtgttgc atatgtcgtc gccggataag ttctggcttg
     3181 gcctgttcga tgtcgtcggc aaacccgagt gggcgcagga tccgcgcttc aacaaccgca
     3241 aatcgcgggt ggaaaactat gacgccatca atgaacagtt ggcgccgatc tttcgcgccg
     3301 gcaagcgcga cgattggctg cgccggctaa tcgccaaaga tgtaccggcc gcgcccatca
     3361 acactctcga tgaagtgttc gccgatccgc aggttaaaac ttacggtttc ccgatcgaag
     3421 tggagcatcc gcagatgggc aaaatgcagc tcatcggcaa cgccgtcgac atgagccgga
     3481 caccgccggc gatcgatcgg ccgccgccgg tattgggcga gcagaccgaa gaggtcttga
     3541 tggcgctagg ctacgatagc gagaagatcg ccaatttacg ccagcaagga gcggtgtgaa
     3601 attaagttgg agtaagggag tgatggagca ctggagtgtt ggggcgtccg gaccccttac
     3661 tgcaccactc caatactcca tcactccagt cccgccggcc ccccatgcgt catcctagct
     3721 tattgccgct ttcgcacgac catcaccatg gtctggcgct ggccctacgc tgccgtaagc
     3781 aggggcttgg ccagatcaag ccgatggggg ccgaggggtt gcgcgagcgc gccaaggagt
     3841 ttttagaatt ttaccgcgct aatctcgtgg ctcatttcag tgctgaagaa caagtcttgt
     3901 tcccgctaat gcgtcaacag gtagccggtt gtggcgccat ggtcgatcag ctggtgcagg
     3961 accatgacca gctgcgccgc gccatgccgc agctcgaatc cggcacgggc ttggcgaagc
     4021 tcatttttga cttgggtgat ctgctggagc gccatattcg cacagaagag cgcgagcttt
     4081 tcccgctttt cgaggcgcat gtggatgcgg cgcaggccgg gctggtcggc gcaaaactac
     4141 aagaactgct caatcctcaa ggcgcgcgat aaccgccctg agcgcggcgc ggtcagtcca
     4201 ttaactttag caacacgtgc cggcggtttt tcgtgtgcgg acgcacctgg ccgggccagc
     4261 ggccgtcttc gatcgccttg tcccaggccg ggctggtgag cacgtcgggg ctttccagct
     4321 cgtagatcgc cgtgtacgtc ggctcgttgg gaatctcgat ggtcttactc tcgccgccga
     4381 tgttcatggt cagcgtgccg cgtttatagc gcgatacgcc aaggacgccg ggaactttaa
     4441 gcagattggg cacatgttct tggtcgtaaa cttcgttgaa gatggcgtcg tgttcagcgt
     4501 cgacatccat gctcgcgatc attaagtatt ttgctttgcc tgccatgatt gagttctcct
     4561 tggggcgctt gccgtgaatt tacatgccta agaagctgcc gagctccggc gccggcgatt
     4621 tgatttcgac gacgatggat tcttcgatgc cctcgacgcc atggggcacg cccttgggat
     4681 ggcggcagac atcgccgggt ccgagaatga actcttcctt gccgacaatc attttaactt
     4741 tgcccttgac gacatacgcg agcgactcgt gattatgcac atgcaatgga gcaccgacgc
     4801 cgggggtgta ataaatctcc aaaaatgtca tctgctcgcc ggtgatgagc ggcttcaccc
     4861 gtaaatcgcc gccgtacatc ggcttgcctt cgatggcttt gacaatgatc gcgtcgacat
     4921 cggcgttctt agatagaatc atttgcggct ccttttagtg gttcggggga tcgcttgagg
     4981 acagggcgga ttctagccac gggttcaatt caagtcaatg taattgagct ggtacggcgg
     5041 tgcatctcgc gtttttcagc gatccttgat aagtcaggcc aagccggtga agcagcggag
     5101 gaaatcatgt tagtgcaagc tttggatcat ttcgtcgtgc cggttaacga catcgtcgag
     5161 gccgaggact tttacgtgca agtgttcggc gccaggatcg ccaaacgcaa cggtcttaac
     5221 gtcaaatcgc aaagcctcgg gccgcatacg ttcatggata tcgccggtaa gcgcatcggc
     5281 gtttacctgc aaaaagaaga gcgcggccag ccggtcgcgc cgcgcggtgc accgacctac
     5341 tcgtttacca ccaccaagca tggcatcgaa gaggtgatgg atgtgctggc caattggaag
     5401 attggcttcg aaggtccgca aaaaaacagc catccgttcg ccacgacaac gctgtttttc
     5461 gccgatcctg cgggtaataa ttttgcggtc tacgtgccga gtgtcgatgg ccgaaacact
     5521 cccccgggag gcgggcgctt gaccggcgtg ggttatttgg agctggaagc gcccaagctc
     5581 gaagattcga ttaaatttta ccaatcggtg ctgggcttcg aactgctcaa ccgcggtcag
     5641 gagccgaaga cggatcgcaa ccaagcgacc atgcgcatgg cgagcgggca atacttaatc
     5701 cttactgaag cgagcttcag ccctaagggt tat
//
LOCUS       DASQKS010000206         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5998601, whole genome shotgun sequence.
ACCESSION   DASQKS010000206 DASQKS010000000
VERSION     DASQKS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5998601"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..946)
                     /locus_tag="VE170_05355"
     CDS             complement(314..946)
                     /locus_tag="VE170_05355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYJ14895.1"
                     /translation="MKLYDISLTLSPESIRWVTAQPLELIGRKRMSKGDTNNSSSIHT
                     SVHAGTHVDAPFHFLPDGITIEALPLETFIGPARVCAAEPGSHITAADVAKANLQGET
                     RVLFKTRNSQLLKKGVYDANFAPFSVDGAKALVDLGVKLVGLDYLSAAGASEQVPVHR
                     AFLDHGVILLEGIDLSEVPAGRYELFCPPVKLAGSDGAPCRAVLRELEGM"
     gene            complement(1068..1943)
                     /locus_tag="VE170_05360"
     CDS             complement(1068..1943)
                     /locus_tag="VE170_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYJ14896.1"
                     /translation="MTSEAAAQVLALITSVLYASALVSARAGMRFSTPTTATLVSILM
                     QNVLLWSAVFATGGVHAVPLSGVVLFTVVGIFQLGVRLFAYTGVEKIGASRSSALQSV
                     SPLISATIAIAVLGEPTSALIVLGTLLVVAGIVLVSWKPDQQIASFRRWHLLLPLGAA
                     FLTGMNHPIRRYVLTMANEPLFFSALMGLVSFGAFLIYLVVSPSSQRLVWHRQALWPF
                     LTTGLSETFSILCIITAISLGRVVIVAPIAASYPVWSLIFAALFLRDVEAINWKVIAG
                     IASVVAGNFAIHLGR"
     gene            complement(2041..>2597)
                     /locus_tag="VE170_05365"
     CDS             complement(2041..>2597)
                     /locus_tag="VE170_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ14897.1"
                     /translation="DEMLKAFARQRKDEIVVAVYMAAQEITHISPSELNYTFIGAMGQ
                     GSSHALGLALGRPDKKIVVFDGDGSLLMNLGSLVTIGNQAPKNYIHCLCENGCYETNG
                     SVLTPNSGNIHFAEFAKTAGYKKTYEIADLAEWEQKLPEILSQEGPLFVTLKVEPGEA
                     YPENFPRLYSTEHREKFRQTLAKS"
BASE COUNT          597 a          771 c          723 g          506 t
ORIGIN      
        1 gaagcatttc ccaaccggca acggcggccg ctcgacagcc atcgaggcgc gccgggaaac
       61 tatcgtcttt aatcggcccg gcgccgccgt ggacaatgat cgatggttga aatcctgtga
      121 tcatgcgcaa gttatctcac gagacattgg gtttagcacg aacatcacga ggttattttt
      181 gcgccctgta agctaagatg gcaggtttca aagccgctcc ttacaatctc gactcttttt
      241 tgttgcgtcg ttggcgttct ttgcggctaa tttttaaacc gcaatttcta tgcgacctat
      301 gcgccctgtg tggtcacatt ccctccagct ctcgcagcac ggcgcggcac ggtgcgccgt
      361 cggacccggc gagctttacc ggcgggcaaa aaagttcgta acgccccgcc ggcacttcgg
      421 acaagtcgat gccttcgagc aggataaccc catggtccaa aaacgcccgg tgcaccggta
      481 cttgctcgct ggcacctgcc gcagacaaat aatctaagcc gactaatttg acgccgagat
      541 cgaccaaggc cttggcgccg tcgacggaga aaggcgcgaa gttggcgtcg taaactccct
      601 tcttgagaag ctgtgaattc cgcgtcttga aaagcacccg ggtttcgccc tgaagattcg
      661 ccttggctac gtccgcggcc gtgatatgcg agcccggctc ggcggcgcac acccgcgccg
      721 gtccgataaa cgtctcgagc ggcaacgctt cgatggtgat gccatcgggc aaaaaatgaa
      781 atggcgcgtc gacatgggtg ccggcatgga cgctggtgtg gatcgacgac gagttgttgg
      841 tgtcaccctt gctcatgcgc ttgcgtccga tcagctccaa cggctgggcc gtcacccaac
      901 gaatcgactc cggcgaaagg gtcaaagaaa tatcataaag tttcatagcg gcatcctcca
      961 gtgaacgatc ttatattgcc gacaaaaccg aagggcaacc gccgagcctt gccctgttgc
     1021 gtgctttgcg gcttatcttc cgatttcgaa tcaatgcttg cggccaatca tcgtcccaaa
     1081 tggatcgcga agttacccgc cactacgctg gcgatgccgg cgatcacttt ccaattgatc
     1141 gcctcgacat cccgcagaaa aagcgccgcg aagatcagcg accagaccgg atagctcgcg
     1201 gcgatcggcg cgacgataac cacccggccg agactgatgg cggtgatgat gcagaggatc
     1261 gaaaaggttt cggacagtcc ggtggtgaga aacggccaaa gcgcctgccg gtgccacacc
     1321 agccgctgcg agcttggcga caccaccaga tagattaaaa atgcgccaaa cgacaccaag
     1381 cccatgagcg cagagaaaaa caacggctcg ttggccatgg tcaagacgta acggcggatc
     1441 ggatgattca tcccggtgag aaaggccgcg ccgagcggca gcagcaaatg ccagcggcga
     1501 aaacttgcga tctgttgatc cggcttccac gaaaccaaga caataccggc gaccaccagc
     1561 aaggtgccga gcacgatcaa cgccgaggtc ggttcgccaa gaaccgcaat ggcgatggtt
     1621 gcactgatca atggactgac tgattgcaac gcgctgctgc gtgacgcgcc gatcttctcg
     1681 acgccggtat aagcgaacaa gcgcacaccc aactgaaaaa ttcccaccac ggtaaaaagt
     1741 accaccccgc ttaacggcac ggcatggacc cctccggtgg cgaacaccgc gctccatagg
     1801 agcacatttt gcatcaggat cgataccaag gtcgccgtgg tcggcgtcga gaagcgcatg
     1861 ccggcccgcg ccgagaccag cgccgaggcg tacaagaccg aggtaatgag cgcgagaacc
     1921 tgcgccgccg cttccgaggt catggctgaa cgaacaggcg gcggcaggca tggaagaagt
     1981 ccagcgccgc gcgtcccaag tgcaactcaa gacccacgac gcgagactgg aaacacggtg
     2041 tcaactcttc gccagtgtct ggcgaaattt ttctcgatgt tcggtgctgt agagccgcgg
     2101 gaagttttcc gggtaagcct cgcccggctc gactttcaat gtcacgaaca gcggtccttc
     2161 ttggctgaga atctccggca gcttctgctc ccactcggcg agatcggcga tctcgtacgt
     2221 cttcttgtag ccggcagttt tagcgaactc ggcaaagtga atattgcctg aattgggcgt
     2281 cagcaccgag ccattggttt cgtagcagcc gttttcgcat aaacaatgaa tatagttttt
     2341 cggcgcttga ttgccgatgg tcactaggga gccgagattc atcagcaagc tgccatcgcc
     2401 gtcgaagacg acgatcttct tatcaggccg gcccagcgcc aagcccaacg catgggacga
     2461 accctggccc atggcgccga taaatgtgta gttaagctcg ctcggcgaaa tatgcgtaat
     2521 ctcctgcgcc gccatgtaaa ccgcgactac gatttcatcc ttgcgctggc gcgcaaaggc
     2581 cttaagcatt tcatctc
//
LOCUS       DASQKS010000207         7641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5999340, whole genome shotgun sequence.
ACCESSION   DASQKS010000207 DASQKS010000000
VERSION     DASQKS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5999340"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VE170_05370"
     CDS             complement(<1..710)
                     /locus_tag="VE170_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HYJ14898.1"
                     /translation="MADSGIRFSIAAYAAWAPGVETPEAWLDWASGRREIDGESEPAL
                     HSMAPMLRRRAGVLDRMALEVAYRCLGDRTGVAAIFCSRHGETARSVQLLTDLAQGLP
                     LSPTNFGLSVHNATGGLFSIARGDHAGSSALAAGASSVEHAAVEACGLLADGEAEVLL
                     VVYERPLPALYAAYQDCREQPYAWAWLMRPPAGAVVSLSWSKAEDHEIMSVERYSPGL
                     EILRFYFHRGRSLDRVCQR"
     gene            complement(777..1160)
                     /locus_tag="VE170_05375"
     CDS             complement(777..1160)
                     /locus_tag="VE170_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14899.1"
                     /translation="MKILLALSLVVLGGCVQARDPQLLAMRQVGDGALTCEQIAVEFK
                     SNTEVALNKITTNRANDRRELVLGILVWPGLFELKNPDGNEANALLDRNIYLREAAKG
                     KSCPAMETWPGQPERYTMNSYWSTL"
     gene            complement(1374..2096)
                     /gene="hisA"
                     /locus_tag="VE170_05380"
     CDS             complement(1374..2096)
                     /gene="hisA"
                     /locus_tag="VE170_05380"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HYJ14900.1"
                     /translation="MLIIPAIDLKDGRCVRLFQGDMDQETIYFEDPLAAARHWLAEGA
                     TFLHIVDLNGAVAGRPFHTQEVRTICGVAGLSVELGGGLRSVDSVQAALDLGVARVVI
                     GTAACDNAELLQTLCKKFPGKIVVGIDARQGKVAVKGWKETTELEAVELAKRCQGDGA
                     SRIIYTDISRDGTRAGVNLDETLKIASAVKIPIIASGGVATLDDIRKLLPLQKYGVEG
                     VVVGRALYNGAFKYTEAASLTA"
     gene            complement(2187..2804)
                     /gene="hisH"
                     /locus_tag="VE170_05385"
     CDS             complement(2187..2804)
                     /gene="hisH"
                     /locus_tag="VE170_05385"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYJ14901.1"
                     /translation="MIAIIDYGMGNLRSVQKGLERVGVDALVTRDVGQIQSARGVVLP
                     GVGAFSACMENLGNFGLVEPIREIVLQQKPFLGICLGFQLLFSESEEFGNQKGLDLFP
                     GKVVGFHANRELKVPHMGWNRINKELDTPFLEGISTGDYVYFVHSFYVAPQDPTIIST
                     TTDYGQSFVSSIATDRLFACQFHPEKSQELGLRILANFGRFAAQQ"
     gene            complement(2858..3445)
                     /gene="hisB"
                     /locus_tag="VE170_05390"
     CDS             complement(2858..3445)
                     /gene="hisB"
                     /locus_tag="VE170_05390"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HYJ14902.1"
                     /translation="MGRTAKVARKTKETDITIDLDVDGSGQASIETGMPFFNHMLDAF
                     SRHGFFNIAIRATGDLEVDYHHTVEDVGLTLGQAFKDALGDKSGIRRFGEASCPLDET
                     LAKVVVDLSGRPYLLYNVKIRPGRVGDFDTDLPHEFFAAFANQLGMNLHIDVPRGENP
                     HHIIEACFKAFARAMDFATQVDARVQGVLSTKGSL"
     gene            complement(3507..4802)
                     /gene="hisD"
                     /locus_tag="VE170_05395"
     CDS             complement(3507..4802)
                     /gene="hisD"
                     /locus_tag="VE170_05395"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYJ14903.1"
                     /translation="MKIRLVKTSDAGFKPLMAKILGRRGTRAGDVEQRVDEIVTTVQR
                     QGDRAVLRYTAKFDHMRLRAAQMEVTPAEIARAVAKVSRKDLSILRLAARRIAVFHRR
                     QMLKSWQYRDPLGMVLGQRITALERVGVYVPGGKACYPSTVLMNAIPAKVAGVKEIIM
                     TSPLGADAAIILAAAHVAGVDRIFRVGGAQAIAALAFGTRTIPKVDKIVGPGNIFVAT
                     AKRMVFGEVNIDSIAGPSEILLLTDDSADPVHVAADMLSQAEHDELAAALCVTTSKAV
                     AHKVQKAIEQQLISTKRRAISLRSLQKYGAIIVARGQKEMIELANAIAPEHIELLVRQ
                     PEKMSRQIRNAGAMFLGPYSAPPLGDYLAGPNHVLPTGGSARFFSPLGTYDFLKRTTI
                     IHAERRGLKALAAKITHLARLEGLDDHARSVEVRFEKRP"
     gene            complement(4853..6121)
                     /gene="murA"
                     /locus_tag="VE170_05400"
     CDS             complement(4853..6121)
                     /gene="murA"
                     /locus_tag="VE170_05400"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYJ14904.1"
                     /translation="MDKIVIQGGQRLHGEIAVSGSKNAALPILISSLLTAEPCTYQSV
                     PHLADIRTTLKLLAGLGGKSEHQRWGAGADDLTLRADQVGNFEAPYDLVKTMRASFLV
                     IGPLVARFGRARVSTPGGCAIGARPIDLHLKGLEALGVIIEQTHGYVEARAAKLRGAK
                     IYLDVPSVGATENLMMAATLAEGRTVIENAAKEPEIDDLAKVLNAMGGHVHGAGSDVI
                     TIDGVSALHGVSHRIIPDRIETGSFVIAAALSGGEVYVKGARADHLEAFLIKLREAGV
                     TWMSDDGGIHVCGGGRIKSVDVKTQPYPGFPTDLQAQMMVLMAVAEGVSVITETIFEN
                     RFMHVPELDRMGADIKLEGNRAVVRGVKELSGAPVMASDLRASVALVVAGLIASGSTE
                     VARVYHLDRGYEQIENKLSQLGARINRVAG"
     gene            complement(6133..7035)
                     /gene="prmC"
                     /locus_tag="VE170_05405"
     CDS             complement(6133..7035)
                     /gene="prmC"
                     /locus_tag="VE170_05405"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYJ14905.1"
                     /translation="MDEVSNKVNGKGLRRLCDELILAVDRLSAGGLDSPRLDAEVLLA
                     HCLTMSREQLLAARDLPIAPAAARHYETLLARRLQREPVAYITGKREFWSRDFAVTSD
                     VLIPRPDTERLVEVSLLCAAQFPASTPLRIADLCTGSGAVAVTLAGELPSAQIYATDI
                     SPAALKIARGNAADHHVAERVQFVTGDLFAALPPRPGVTFDLIVSNPPYVRRDEIFTL
                     APEVSRWEPRVALDGGIDGLEFYRRIVAVAPDYLAARGALVLEIGADMASAVVALCGR
                     FREIAVHQDYAGKDRVLSARLENI"
     gene            complement(7049..>7641)
                     /locus_tag="VE170_05410"
     CDS             complement(7049..>7641)
                     /locus_tag="VE170_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HYJ14906.1"
                     /translation="GYKEIILSIEGQGAYSRLKFEGGVHRVQRVPVTEGSGRIHTSAV
                     TVAVLPEADEVELVIDPKELRIDVFRSSGPGGQSVNTTDSAVRITHIPTGIVISCQDE
                     KSQHKNRAKGMKILRARLLEKKQDEQASEIAASRKSMVGSGDRSERIRTYNFPQGRLT
                     DHRINLTIYQLERVMEGQIDEVVEGLITHYQAAALK"
BASE COUNT         1653 a         2355 c         2045 g         1588 t
ORIGIN      
        1 cgctggcaga cgcgatccag agagcggcct cggtggaaat aaaagcgcag gatctccaga
       61 ccgggagaat aacgctcaac gctcatgatt tcatggtctt ctgccttaga ccaggaaagc
      121 gaaacaacag cgccggccgg cggccgcatc aaccacgccc aggcataggg ttgttcacgg
      181 caatcctgat atgccgcata gagcgccggc aagggccgct cgtaaaccac cagcaagacc
      241 tcggcctcgc cgtcggcaag caatccacaa gcttcgaccg ccgcatgttc cacgctgctt
      301 gcgcccgcgg ccagcgcgct gctgccggca tggtcgccgc gggcaatgga gaacaaacct
      361 ccggtggcat tgtgcaccga caagccgaaa ttagtcggag aaagcggcag gccctgggcc
      421 aaatcggtca agagctgcac cgaccgcgca gtctcgccat gacgcgagca aaagatcgcc
      481 gccacgccgg tgcgatcgcc gagacatcga taagccactt cgagcgccat ccgatcaaga
      541 acgccagcgc gccggcgcaa catcggcgcc atcgaatgca acgccggctc cgactcgccg
      601 tcaatctcgc gtcgacccga cgcccagtcg agccaagcct ccggggtttc gacccccggc
      661 gcccacgcag cgtaagcggc gatggaaaat ctaatcccgc tgtcggccac gtccggtaca
      721 atcctaatgt cgcggcaacc gtcgtagtct tagactgaaa aacgctgaga tacggttcat
      781 agcgtcgacc agtaactgtt catcgtatag cgttccggct gccccggcca ggtttccatc
      841 gccgggcacg attttccctt ggcggcttcc cgcaggtaaa tattgcggtc gagcaatgca
      901 ttagcctcat tgccatccgg gtttttcagt tcgaagagcc ccggccacac gagaattccc
      961 aaaactaatt cgcgccgatc gttcgcccgg ttcgtggtga ttttattcag cgccacctcg
     1021 gtgttcgatt tgaactcaac ggcgatctgt tcacaggtca gagcgccatc accgacttgc
     1081 ctcatggcga gcagttgcgg atcgcgtgct tgcacgcatc cgcccaggac gactaacgac
     1141 agcgccaaca aaattttcat atcagctcct agtgattgtc cgagattatt gtttgcgatt
     1201 cgtctgcggc tcttggccaa gatcgccgta acgatccgcg tcactacact tgtttaatta
     1261 ttgccaatca tagtttgaac gcgtctgatg ggcaactgag tttatataat agtattcaat
     1321 tcgcgcaccg caaccgcaca agcgtgataa ttggcaaacc gtcgggtgac aggctatgca
     1381 gtcaagcttg ccgcctctgt gtatttgaac gctccattat agagcgcgcg gccgaccacg
     1441 acaccttcca cgccatattt ttgcagcggc aacaacttgc gaatatcgtc gagcgtggcg
     1501 acgccgccgg aggcgatgat gggaatcttt acggcgctgg cgatttttag agtctcatcc
     1561 agattcactc cagctctcgt gccgtcgcgg ctgatgtcgg tatagataat ccgcgaggcc
     1621 ccgtcacctt gacagcgctt cgctagctcg acagcctcca actcggtggt ttccttccag
     1681 cctttgaccg cgactttgcc ttggcgggca tcgatgccga cgacgatttt accaggaaat
     1741 ttcttacata aagtctggag cagctcggcg ttgtcgcaag ccgcggtgcc gatgaccacg
     1801 cgggctacgc ctaagtccag cgcggcttgc acggagtcga cggatcgcaa cccgccgccc
     1861 agttccactg aaagtccggc cacaccgcag atcgttctca cttcttgcgt gtgaaacggc
     1921 cggcctgcca ccgcgccgtt caagtcgaca atgtgcagaa aggtcgcccc ctcggctaac
     1981 caatgcctgg ccgcggcaag cgggtcttcg aagtaaatcg tctcttgatc catgtcgccc
     2041 tggaacaaac gcacgcaacg gccgtccttc aagtcgatgg cgggaatgat caacataaat
     2101 tcagatagaa gagaattaaa aatggaaaac tatcagaccc tcggagcgtt atttgtttca
     2161 ctcatttttg ctttttcact cttcactcac tgctgcgccg caaaccgccc aaaattggcc
     2221 aaaatccgca gtcctaattc ttggcttttc tcaggatgaa actggcaggc gaacagcctg
     2281 tcggtggcga tactcgaaac gaaggattgg ccgtaatcgg tggtggtgga aatgatcgtt
     2341 ggatcttgcg gcgcgacgta aaacgagtgg acgaaataaa catagtcgcc ggtcgagatg
     2401 ccttcgagga acggcgtgtc tagctctttg ttaatgcggt tccagcccat atgcggcact
     2461 ttcaattccc gattcgcgtg aaaaccaacc accttgccag gaaaaagatc gagccctttt
     2521 tgattaccga attcttcgct ctccgagaac aacaattgaa atcccaagca aattcccaga
     2581 aacggttttt gctggagaac gatttcacgg atcggttcga cgaggccaaa attgccgaga
     2641 ttctccatac aggcgctaaa tgcaccgact cccggtaata caacgccgcg agccgattga
     2701 atctggccga cgtcgcgggt gactagagcg tcgacgccga ctcgctccaa tcctttctgc
     2761 acgctgcgca gattacccat cccgtaatcg atgatcgcaa tcatattctc ttagaatgga
     2821 acgcttggaa cctctggaac atttggaacc ggttcgatta taagctcccc ttggtcgaca
     2881 acacgccctg cacgcgcgca tcgacctggg tagcgaaatc catggcccgg gcaaacgcct
     2941 tgaagcaggc ttcgataata tggtgcggat tctcgccgcg cggcacatcg atatgcaggt
     3001 tcatccccaa ctgattggca aaggcggcga aaaattcatg gggcaagtcg gtgtcgaaat
     3061 cgccgacgcg gccgggacga atcttgacgt tatacaagag atagggccgg ccgctcaaat
     3121 cgaccaccac cttggccagc gtctcgtcga gcggacagct cgcttcaccg aagcggcgga
     3181 tgcccgactt gtcaccgaga gcgtctttga acgcttgccc cagtgtaagc ccgacatctt
     3241 ccaccgtgtg gtgatagtcg acttcgagat cgcccgtggc gcggatggcg atattgaaaa
     3301 aaccatgacg gctaaacgcg tcgagcatat ggttgaaaaa cggcatgccg gtttcgatac
     3361 tggcttggcc gctgccgtcg acatccaggt cgatcgtgat gtcggtctct ttagttttac
     3421 gcgctacctt cgctgtccgt cccatgatta tctcccaagt ttcatcgcct cttcaaacgt
     3481 gctttgacat gaaacccaac ccgcagttag ggcctcttct cgaaacgcac ttccaccgat
     3541 cgtgcgtgat cgtccagccc ttccaaccga gccaaatgag taatcttcgc cgccagcgct
     3601 ttcaagccgc gccgctcggc atggatgatg gtcgtgcgct tgagaaaatc ataggtgccg
     3661 agcggcgaaa aaaaccgcgc gctgccgccg gtggggagga catgattggg tccggccaga
     3721 taatcgccca agggcggcgc cgaataggga ccgagaaaca tcgccccggc gttgcgaatt
     3781 tgccgcgaca ttttttcagg ctggcgcacc aacaattcaa tatgctcagg cgcgatggca
     3841 ttcgccagct cgatcatttc tttttgtccg cgcgcgacga tgatcgcgcc gtacttttgg
     3901 agcgagcgca gcgagatcgc ccggcgcttg gtgctgatta actgctgttc tatggccttt
     3961 tgaaccttgt gcgccaccgc tttcgacgtc gtcacacata acgcggcagc caattcatca
     4021 tgctccgcct gtgacagcat gtccgctgcg acatggaccg gatccgcgga atcatcggtc
     4081 aaaagtaaaa tctcgctagg cccggcgatg gagtcaatat tgacctcacc gaagaccatg
     4141 cgtttagccg tggcgacgaa gatatttccc ggcccgacga tcttgtcgac tttagggatt
     4201 gttcgagtac cgaaagccaa cgcggcgatc gcctgagcgc cgccgacacg aaagatccga
     4261 tccactcccg ccacatgagc ggcggcgaga atgatcgcgg cgtcggcgcc gaggggtgaa
     4321 gtcatgataa tctctttgac gccggcgacc ttggcgggaa tcgcgttcat caaaacagtc
     4381 gacggataac aggccttgcc gccgggcacg taaacaccga cccgttcgag cgccgtgatg
     4441 cgctggccga gcaccattcc caggggatcg cgatactgcc agctttttag catctgccgg
     4501 cggtgaaaca ccgcgatccg tctggcggca agccgcagaa tgcttaaatc tttgcgcgaa
     4561 actttcgcca ccgcacgggc gatctccgcg ggagtcactt ccatttgtgc cgcgcgcaat
     4621 cgcatatgat cgaactttgc cgtgtagcgc agcacggcgc ggtcgccctg gcgctgcacc
     4681 gtcgtgacaa tttcgtcgac acgctgctcg acatcgccgg cccgagtgcc gcgccgcccg
     4741 agaatcttcg ccatcaaggg cttgaaccca gcgtcggaag tttttactaa acgaatcttc
     4801 attctgacct tgaacttttg aacccttgaa ccttgaaccg aggaaccgaa ttttatcccg
     4861 cgacgcgatt tatccgcgcg cccagttgcg aaagtttatt ttcgatctgt tcgtagccgc
     4921 gatcgagatg ataaacgcgg gcgacttcgg tgctgccgct ggcgatcaaa ccggcgacca
     4981 ccagcgctac gctagcgcgc aagtcactgg ccatgaccgg tgcgccggac agctctttga
     5041 cgccgcgcac cacggcgcgg ttgccttcaa gcttgatgtc ggcgcccatg cggtcgagtt
     5101 ccggcacatg catgaaacgg ttctcgaaaa tcgtctcggt aatcacgctg acgccctcag
     5161 ccaccgccat cagcaccatc atctgcgcct gcaagtcggt gggaaaaccg ggatacggct
     5221 gagtctttac atcgacactt ttgatccggc cgccgccgca cacatggata ccgccatcgt
     5281 cgctcatcca agtcacgccg gcttcgcgca atttgatcaa aaaagcttcg aggtgatcgg
     5341 cgcgcgcgcc tttgacatag acctcgccac cactgagcgc cgcggcgatg acaaagctgc
     5401 cggtttcgat gcggtcggga attatccggt ggcttacgcc atgcaaggcg cttacaccgt
     5461 cgatcgttat gacgtcgctt cccgcgccat gaacatgccc ccccatggca ttcaatactt
     5521 ttgccaagtc gtcgatctcc ggctccttgg cggcattttc aatcaccgtc ctgccctcgg
     5581 ccaaggtcgc cgccatcatg agattttccg ttgcgcccac cgagggcacg tccaaataaa
     5641 ttttcgcgcc gcgcagtttc gccgctcgcg cttcgacata accatgagtc tgctcgatga
     5701 taacgccgag cgcttcgagc ccctttaaat gcaggtcgat gggacgcgcc ccgatggcac
     5761 aacctcccgg cgtcgacacc cgggcccggc cgaagcgcgc caccagcggg ccgatgacca
     5821 ggaacgaagc gcgcatggtc ttgaccaaat catagggcgc ttcgaagttg ccgacttgat
     5881 cggctcgtaa agtgagatcg tcggcgccgg caccccagcg ctggtgctca ctcttgccgc
     5941 cgaggccggc gagcaatttc aaagttgtcc gaatatcggc gagatgcggc acgctttgat
     6001 aggtgcaagg ctcggcggta agcagcgacg aaattagaat cggcagcgcc gcattcttcg
     6061 aaccgctgac ggcgatctcg ccatgcaacc gctggccgcc ttgtatgacg atcttatcca
     6121 cccgtcagtt ccctagatat tctcaagccg cgccgaaagg acgcgatctt tgccggcgta
     6181 gtcttgatga accgcgatct cccgaaagcg tccgcaaagc gccacaaccg cactcgccat
     6241 atccgcaccg atttccaaca ccaacgcacc gcgcgcggcc aaatagtccg gcgccacagc
     6301 gacgatccgc cgataaaact ccaagccatc gatgccgccg tccaacgcga ctcgcggctc
     6361 ccaacggctc acttccggcg ccagcgtaaa aatttcatcg cgtcgcacat agggtggatt
     6421 gctgacgatc aaatcaaaag taacgccggg tcgcggcggc agcgcggcga acagatcgcc
     6481 ggtgacaaat tgcacgcgct ccgcgacgtg atgatccgcc gcgttgccac gggcgatttt
     6541 caacgccgcc ggcgaaatgt ccgtggcgta gatttgtgcg ctcggcaact cacccgccag
     6601 agtcaccgcc accgcgccgc taccggtaca caagtcggcg attcgcagtg gcgtcgacgc
     6661 cgggaactgt gcggcgcata atagtgatac ttcaaccaag cgctcggtat ccggccgcgg
     6721 aatcaacacg tccgacgtta ctgcaaaatc ccgagaccaa aattcccgct taccggtgat
     6781 gtaagctacc ggctcccgct gcaagcgccg cgctagtaaa gtttcataat ggcgcgccgc
     6841 tgctggagcg atcggcaaat ccctggcagc cagcaactgt tcgcggctca tagttaaaca
     6901 atgggccagc agcacttcgg catcgagacg cgggctgtca agcccgccag cggacaatcg
     6961 atcgactgcg agtattagtt catcgcatag ccgccgcaga cccttaccgt tgactttgtt
     7021 ggaaacctca tccatcgcta taatttaatt acttgagcgc tgcagcttga tagtgggtga
     7081 tcaagccttc caccacttca tcaatctgcc cttccatcac gcgctcgagc tggtaaatcg
     7141 tcaaattgat ccggtgatcg gtcaaccgcc cctggggaaa gttataggtc cggatgcgct
     7201 cgctgcggtc gccgctgccg accatggatt tgcgcgatgc ggcaatctcc gatgcctgct
     7261 cgtcctgttt cttttccaac aagcgcgcgc gcaaaatctt catgcctttg gcgcggtttt
     7321 tatgctgcga tttttcatcc tggcaggata tcacgatgcc ggtgggaata tgagtaatgc
     7381 gcaccgccga gtcggtggtg ttgacgctct ggccgcctgg tcccgacgag cgaaatacat
     7441 cgatgcggag ttctttagga tcgatgacga gttccacttc gtcggcttcc ggcaacaccg
     7501 cgacggtcac tgccgaagta tgaatgcgtc ccgagccttc ggtcactggc acgcgttgga
     7561 cacggtgcac gccgccctca aatttaagcc ggctgtaagc cccttggccc tcgatactga
     7621 ggatgatttc tttatagccg c
//
LOCUS       DASQKS010000208         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5999703, whole genome shotgun sequence.
ACCESSION   DASQKS010000208 DASQKS010000000
VERSION     DASQKS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5999703"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VE170_05415"
     CDS             <1..578
                     /locus_tag="VE170_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYJ14907.1"
                     /translation="LARDNRTLGNFRLEGIQPAPRGAAQIEVTFDTDANGILTVAAKD
                     KQSGKEQKITISGSTQLAKEEIDRMVADAQANSADDRKRREEIEARNGADSMAYQVER
                     QISELGAGVAPNEKARAEQLIGDIRELVKNNSTDLARLRQLTSDLQQVAYGLASAAAT
                     ETASARPKGDGSPGAQRGGGDDVIDAEFKET"
     gene            621..1109
                     /locus_tag="VE170_05420"
     CDS             621..1109
                     /locus_tag="VE170_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYJ14908.1"
                     /translation="MENTDQNKKPMAAGAPAEIEQLKQELAREHDMYLRALADFDNYR
                     KRVERERATATRGGKREVMRPLLEVVDGFDRALNHIDDAPSSISQGVRALYRQLLGVL
                     EAQGVTPLQSVGETFNPEVHDAIGLVESGEVEPGAVAEELQRGYRWGDEVLRPARVRV
                     AR"
     gene            1182..2213
                     /locus_tag="VE170_05425"
     CDS             1182..2213
                     /locus_tag="VE170_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYJ14909.1"
                     /translation="MAVTFRDYYEVLGVAKSASADEIKSAYRKLARKYHPDVNPGDKA
                     AEDRFKEINEAYEVLSDTDKRKRYDQLGQNWKAGEEFRPPPGWEGARGGGVGDFNDMF
                     GGGGGRSGGFSDFFESLFGGRRSASGGAGFALRGQDAEAEIALTLEEAHRGVKRNISL
                     QVMESCSDCRGAGMKDNDVCTTCRGAGFTRRPKSLEVTIPAGVRDGSVIRLAGQGEPG
                     SNGASAGDLYLRVTMRPHRLFNVVGDSDVQIELPVAPWEAALGAKIAAPTLDGPVEMK
                     IPAGAQGGQRLRLRGQGLSRRGGGRGDEYVKLKIVIPPKLDSKEKELFEKLAAESHFN
                     ARELTEGNP"
     gene            2210..2527
                     /locus_tag="VE170_05430"
     CDS             2210..2527
                     /locus_tag="VE170_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HYJ14910.1"
                     /translation="MMSVQRYDIVLCRRDAQLVTLETLAAHAGMHPALVDRFVESGLI
                     EPVEWVGTTLLFDAAAIARLRMIGRLRESLGINLAGIGVIVNLLDRLCALQRENEAQR
                     NRS"
     gene            2564..>4104
                     /locus_tag="VE170_05435"
     CDS             2564..>4104
                     /locus_tag="VE170_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYJ14911.1"
                     /translation="MDINRLTQKTQEAVGSAQTKAARYSHQQVDVEHLVAALLEQENG
                     LASSIFAKAGVTADALKRRIEQELERMPKVSQSGSAPDQIYITGRLNNLLAQAEDEAK
                     KLKDEYVSIEHLLLAMTDDRGAAGRVLRDLGITREKLAAALKDIRGNQRVTSQNPEAT
                     YEALQKYGRDFTKLAAEGKLDPVIGRDEEIRRVIQVLSRRSKNNPVLIGDPGVGKTAI
                     VEGLAQRILRGDVPESLKNKRLISLDMGALIAGAKYRGEFEERLKAVLKEVQESQGEI
                     ILFIDELHTVVGAGASEGSMDASNLLKPLLARGELHCIGATTLDEFRKYVEKDRALER
                     RFQPVFVDQPSVEDTISILRGLRERYEVHHGVRIKDAALVSAAVLSHRYITDRFLPDK
                     AIDLVDEAAAKLRTEIESMPTQLDEVSRRVMQLEIEREALRKESDAASRERLTKLEKE
                     LAELKTQQQELRARWELEKQAVGRLRAIKEEIEKTKLAIEQAEREYDLNRVAELKYGK
                     LAQLER"
BASE COUNT          947 a         1144 c         1306 g          707 t
ORIGIN      
        1 agctggcacg cgataatcgc actctcggaa acttccgcct cgaaggcatt cagccagcgc
       61 cgcgcggcgc agctcagatc gaagtgacat tcgacacgga cgccaacggc atattgacag
      121 tcgcggcgaa agataagcag agcggaaaag agcagaagat caccatcagc gggtcgaccc
      181 aactggcaaa ggaagagatc gaccgaatgg tggccgacgc ccaggccaac tccgccgacg
      241 atcgcaagcg gcgcgaagag atcgaagcgc gcaacggtgc cgactcgatg gcctaccagg
      301 tggaacggca gatcagcgag cttggcgccg gcgtggcgcc gaacgaaaaa gcgcgcgcgg
      361 agcaattgat cggcgacata cgcgaactgg tgaagaataa ctccacggat ctcgcgcggc
      421 tgcgccagct gaccagcgat ctgcagcagg tcgcctacgg tttggcgtca gcggcggcaa
      481 ccgagacggc ttccgcgcgc cccaaaggcg atggatcgcc cggggctcaa cgcggcggcg
      541 gcgatgatgt gatcgatgcc gaattcaaag agacgtaaac aacttgagta atcgattact
      601 catcactcga gtgattttcc atggaaaata ccgaccaaaa taagaaaccc atggcggccg
      661 gcgcgccagc cgagatcgaa caactcaagc aagagttggc gcgcgagcac gacatgtatc
      721 tacgcgcgct ggccgatttc gacaattatc gtaaacgggt cgagcgcgag cgcgccaccg
      781 ccacccgcgg cggcaagcgc gaggtcatgc gtccgttgct tgaagtggtg gacggctttg
      841 accgcgcgct caatcatatc gacgatgcgc catcatcgat atcccagggt gttcgggcgc
      901 tgtaccgcca attattgggc gtgctggaag cgcagggagt aacaccattg caaagcgtcg
      961 gtgaaacgtt caaccccgag gtgcacgatg ccatcggctt ggtcgagagt ggagaggttg
     1021 agccgggcgc agtcgccgag gagttgcaac gcggctaccg atggggtgat gaagtgctcc
     1081 ggccggcgcg cgtgcgagtg gcgcgctgaa ccgcggcgca atcgacggcc tgggtgaaaa
     1141 gccaacaatg atatcgtcga caagcggccg gtaacttaat tatggcggtc acgtttcgag
     1201 attactatga agtcctcggt gttgccaaat ccgctagcgc ggatgagatc aaatcggcat
     1261 accgcaaact tgctcgcaag tatcacccgg acgtcaatcc gggagataaa gctgccgaag
     1321 acaggtttaa agaaatcaat gaagcgtacg aagttctgtc cgacacggac aaacgcaagc
     1381 gctacgacca attgggccag aattggaaag ccggcgaaga gttcaggccg ccgccgggct
     1441 gggaaggcgc ccgtggagga ggagttggcg actttaacga tatgtttggc ggcggtggcg
     1501 ggcgcagcgg ggggtttagc gatttctttg agagcttgtt cggcggccgg cgcagtgctt
     1561 ccggcggcgc cggctttgcc ctgcgcggac aggatgccga agcggagatt gcgctcacgc
     1621 ttgaagaggc ccatcgcggc gtcaaacgca atatatcttt acaggtaatg gaatcctgca
     1681 gcgattgtcg aggcgccggc atgaaggaca acgacgtatg cactacctgc cgcggtgccg
     1741 gctttactcg tcgaccgaaa tcactcgaag tgacgatacc cgccggcgta cgcgacgggt
     1801 cggtgatccg cttggcgggg caaggcgagc ccggctccaa cggcgcatcc gccggtgatc
     1861 tttatctgcg cgtaacgatg cgaccgcatc gtctcttcaa cgtcgtcggc gatagcgatg
     1921 ttcaaatcga gctgccggtg gcgccatggg aggcggcgct cggggccaag atagccgcgc
     1981 cgacgctcga tggtccggtg gaaatgaaaa ttcccgccgg cgcccagggc ggccagaggt
     2041 tgagactgcg cggtcaggga ttgagtcggc gcggcggtgg tcgcggcgac gaatatgtga
     2101 agctcaagat cgtcattccg cccaagttgg attccaaaga gaaagagctt ttcgaaaagc
     2161 tcgcagctga atcgcatttc aatgcgcgcg aattgacgga gggaaatcca tgatgtcggt
     2221 gcagcgctac gatatcgtgc tgtgccggcg tgatgcgcag ctggtgacac tggaaacact
     2281 tgcggcgcac gccggcatgc atcctgcgtt ggtcgaccga tttgtcgagt ccggactgat
     2341 cgagccggtc gagtgggtcg gcacgacatt actcttcgat gctgccgcga ttgcccgcct
     2401 acgcatgatc ggccggctgc gcgaatcttt gggcatcaac ctcgcgggca taggcgttat
     2461 tgtcaatttg ctggaccgtc tatgtgcgct acaacgcgaa aacgaagcgc aacgaaaccg
     2521 gtcttgacga aggagatcaa gtagcaatta cggaggattt ccgatggaca tcaaccggct
     2581 tacgcaaaaa acgcaagaag cggtcggctc ggcgcagacc aaagcggcgc gctacagcca
     2641 tcagcaggtt gacgttgagc atctggtggc cgcgctgctg gaacaggaaa acggcttggc
     2701 atcttcgatt ttcgccaagg ccggcgtcac ggcggatgct ctgaagcggc ggatcgagca
     2761 ggagcttgag cgcatgccca aggtgtcgca atccggcagt gctcccgatc agatctatat
     2821 taccggtcgt ttgaataact tgctcgcaca agccgaagat gaagccaaaa agctcaagga
     2881 tgaatacgtt tccatcgagc acctgctcct ggccatgacg gatgacaggg gcgccgccgg
     2941 tcgcgtgctc cgggatttgg gaatcacgcg cgaaaaattg gccgccgcgc tcaaagacat
     3001 acgcggcaat caacgtgtca cgtcgcagaa tccggaagca acctacgaag ctttgcaaaa
     3061 gtacggccgg gacttcacca aactcgccgc cgaaggcaag ctcgatccgg tgatcggccg
     3121 cgacgaggaa attcgccgcg tgatccaagt actctcacgg cgcagcaaga ataatccggt
     3181 gctcatcggc gaccccggcg ttggcaagac cgccattgtc gaggggctgg cccagcgaat
     3241 tttgcgcggc gatgtgcccg agagcctgaa gaacaaacgg ctgatttctc tggacatggg
     3301 cgcgctcatc gccggcgcga aataccgcgg cgaattcgaa gagcggctca aagcggtgct
     3361 taaagaggtg caggaatccc agggagagat cattcttttc atcgacgagt tgcacaccgt
     3421 ggtgggtgcg ggcgcctcgg aaggctcgat ggacgcatcc aacttactta agccgctgtt
     3481 ggcgcgcggc gagctgcact gtatcggcgc cacgaccttg gacgagtttc gcaaatatgt
     3541 cgaaaaggac cgcgccctgg aacggcgctt ccagcctgtg ttcgtcgacc agccgagcgt
     3601 cgaggatacg atctcgatcc tgcgcggcct gcgcgagcgc tacgaagtgc atcatggcgt
     3661 gcgcatcaaa gatgccgcgc tagtttcggc tgccgtgctg tcacatcgct acatcaccga
     3721 ccgcttcttg ccggacaagg cgatcgatct cgtcgatgag gcggccgcca aactgcgcac
     3781 cgaaatcgag tcgatgccga cccagctcga tgaagtgtcg cgccgcgtca tgcaattgga
     3841 aatcgagcgg gaggcgctgc gtaaagaaag cgacgcggcg tcgcgtgaac ggctcaccaa
     3901 actggagaaa gagttggcag agctaaaaac ccagcagcag gaactgcgag cccgctggga
     3961 gctggaaaaa caggccgtcg gtcgtctgcg cgccatcaaa gaagaaatcg agaaaaccaa
     4021 gttggccatc gagcaggcgg agcgcgagta cgatctcaat cgcgtagccg agcttaaata
     4081 tggcaagctg gcgcagctgg agcg
//
LOCUS       DASQKS010000209         6353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5144476, whole genome shotgun sequence.
ACCESSION   DASQKS010000209 DASQKS010000000
VERSION     DASQKS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5144476"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VE170_05440"
     CDS             <1..355
                     /locus_tag="VE170_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ14912.1"
                     /translation="RQTNPRLTESFAMTLAQAAMKQNDAGKWLWKFDPLHRTASPQPF
                     YTAQAIEFLRRIACPVLIVDGKQSRQTKRTDKQPRYDAIADRRQVVIDGAGHMVHQDN
                     PTALVAALLPFLQKS"
     gene            523..975
                     /locus_tag="VE170_05445"
     CDS             523..975
                     /locus_tag="VE170_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14913.1"
                     /translation="MRKLKIPQRKPGAPRKRQTDEAPASEPPAARETSLTKSAIPNVD
                     LTLKHFQRRRDDGVARSDGKRLYLRVGDEVNHRRYEEWGIGVVMECMTSSVPGGTCLA
                     RVRFQDGQLRCFNNDLDNEACCYYFGVRRYWNPTHGVDAIRERLFGRG"
     gene            977..1297
                     /locus_tag="VE170_05450"
     CDS             977..1297
                     /locus_tag="VE170_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14914.1"
                     /translation="MKNPILIAVTALTLVALTPPILRAEDNIEKAGVATGVSAGNMWF
                     LPIKAIVMSVGAVSGALSYLVTGGNTELTQQIWRDTMQEPYIITPELARTGVGSRPEL
                     EAKK"
     gene            complement(1438..2172)
                     /locus_tag="VE170_05455"
     CDS             complement(1438..2172)
                     /locus_tag="VE170_05455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ14915.1"
                     /translation="MAQTIENIVDPNDPGLTSEMVKFTSPGGDVNAYVSRPKDGKNLG
                     TVIVIHENRGLVGHIKDVTRRFAKDGFAAIGVDLMSRIGGSDKWNGSDEATKEIQKIN
                     GAMAAEDLTAAVAYMKKQNYVNGKVGVVGYCWGGGQSLNFATKCKDLNAAVVYYGRNP
                     DPLDSVANIPCPVMGNYAGDDPNIMPGVEPLKAAMAKAGKSFDCKVYDGAKHAFNNNT
                     NADRYNPDAAKDAWTRTTSFFKANLS"
     gene            complement(2346..2699)
                     /locus_tag="VE170_05460"
     CDS             complement(2346..2699)
                     /locus_tag="VE170_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYJ14916.1"
                     /translation="MPIYEYSCQKCGVVEVTQRITEKPLAKCPKCKSKVKKLISNTSF
                     QLKGTGWYVTDYARKGQNGEAKSDNASKPASSSSSSADSKSESKSESKSETKKGDSSS
                     TSTDKSTSTSSTAST"
     gene            complement(2794..3159)
                     /locus_tag="VE170_05465"
     CDS             complement(2794..3159)
                     /locus_tag="VE170_05465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14917.1"
                     /translation="MEFLNLQEEMAKGKRKIDLYGTDNFRSWLLYFVPGDGTDMHYHA
                     SPETFLVFSGNGVVKGLKGEEKPIKQHDVLCLAAKDYYQIVNTGTEPLVLFGNRSEAF
                     GGAHILADGRDRSKLHQGA"
     gene            3300..4043
                     /locus_tag="VE170_05470"
     CDS             3300..4043
                     /locus_tag="VE170_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ14918.1"
                     /translation="MLKLNESQAKEYIAELADKCDRRWKQTVDKTKFMDELKEGKLKK
                     ETIQLFYKNWGAFVPVINSVYTAAFYKHLWFFVKNVDLMEVYTQKVLDEFGHPSPPGH
                     IQILMSTGAALGLTKEEVLLAPMLPQCRALPDFHRTLINDGQIQEYWFSVLWEHPFGE
                     SCLDFFKSLTRYYGLTTAEASYFSKHHEADTMDHLDRMAHGAVTQHVLVRLLQEGINE
                     RPGYSAEYCAVTPADLNKMFMDGCYAATH"
     gene            4207..5628
                     /locus_tag="VE170_05475"
     CDS             4207..5628
                     /locus_tag="VE170_05475"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ14919.1"
                     /translation="MAFSDLRAFIDGTARIGELKLIDGADWNLEIGCLTELMAEQEGP
                     LLLFDSVPGYPKGFRIAANALATARRFALALGLPTDAPKLEILRAWRNKIRDLKPFAP
                     VEVATGPVLENILEGDRIDLGIFPTPKWHELDGGRYIGTGDMVVVKDPDSGWINVGTY
                     RSCIAGKDRVTLWIIEQKHGKQIARKYWHAGKPCPVAIVLGCEPATWMAAPSAAKAGV
                     SEYEYAGALRSAPLEVIRAPYTGLPVPATSEIVVEGEMPPPEEESHSEGPFGEWPGYY
                     THSGEECVVRVKRILHRNDPILLGNPPLLPITERYGIPLFAARIWDHLEQSGVSDVQG
                     VWCHCHTLMVVVSLKQRFAGQALQALTAVAGMMTGASMYRYYIAVDDDIDPVDLKQVI
                     WAMCTRVDPAESVQVLKSWTSDLDPRLAPEKRERGDFTMGRMLIDACKPFHWRDKFAV
                     ANKFSADKREEIWEKWAPKLGLK"
     gene            5677..>6353
                     /locus_tag="VE170_05480"
     CDS             5677..>6353
                     /locus_tag="VE170_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYJ14920.1"
                     /translation="MALWILLLLTAQLFVPDAQAAGEVLAEIDGVAITAEEVEKPLVS
                     QLSRLDEQIYALKSQKLDALIDEKLLAKQAVERMLTVAALLDAEVTRKVGSITEQEID
                     EFYQRNKAQINAELAQVREQIRVYLQNQQLAAKREEFLKSLRSQVKVIVHLKPPQVHR
                     VEVSVRGAPFKGGEQAPVTIVEFSDFHCPFCTRVTSTLKQLESMYGEKIKLVFRDFPI
                     EALHPGAT"
BASE COUNT         1448 a         1839 c         1730 g         1336 t
ORIGIN      
        1 gcgccagacc aatccgcgct tgacggagag ctttgccatg acgctggcac aagccgccat
       61 gaaacaaaat gacgctggca aatggctatg gaaattcgat ccgctgcacc gcacggcgtc
      121 gccacagccg ttctacaccg cccaggccat cgaatttctc cgccgcatcg cttgcccggt
      181 gctgatcgtc gacggcaagc aaagccggca gacgaaacgc accgacaaac aaccgcgcta
      241 cgacgccatc gccgaccgga gacaggtcgt tatcgacggt gccggccata tggtgcatca
      301 ggacaaccca acggcattgg tggcagcact gttaccgttc ttgcagaaat cgtaaccctc
      361 gtgacaatca cggcccgacc accgacgccg acaaaaaatt aatcttggtt ggagctcggg
      421 gaccttgact attgagccgg atttttggga ttatgtggca aatgaaatcc cggccggctg
      481 ccggagaaat ttaccgggca tgtgagtcga aggattagcc ccgtgcgcaa actcaagata
      541 ccgcaaagaa aacccggcgc gccgcgcaag cgccagaccg atgaggcgcc agcgagcgaa
      601 ccgcccgcgg cgcgcgaaac ttcactgacc aagtccgcca tccctaatgt cgatctcact
      661 cttaaacact tccagcgtcg ccgcgatgac ggagttgcgc gcagtgacgg caaacgactt
      721 tatctgcgcg tcggcgacga ggtcaatcat cggcgctacg aagaatgggg tatcggcgtg
      781 gtgatggaat gtatgacctc gtcggttccc ggcggcactt gtctggcccg ggtgcgtttc
      841 caagacgggc agttgcgttg ctttaacaac gatctcgata acgaagcttg ctgttactat
      901 ttcggcgtgc gacgatattg gaatcccacc catggcgtcg acgccatccg tgaaagactg
      961 ttcggacgag gatgaaatga aaaacccgat actcatagcg gtgacagcgt taaccctagt
     1021 tgctctaacg ccgccaatcc tgcgggccga ggacaacatc gaaaaagccg gcgtcgccac
     1081 cggcgttagc gccggcaaca tgtggttcct gccgatcaaa gcgatcgtta tgagcgtcgg
     1141 cgcggtcagc ggcgcacttt cctacctcgt caccggcggc aacaccgaac tgacccagca
     1201 aatctggcgc gacaccatgc aagagccgta tattattacc ccggagcttg cccgcactgg
     1261 cgtcggcagc cggccggaac tcgaagcaaa gaaataactc aattatcggc gctgtcggtg
     1321 cgcggacaag gtatcccaga ccgcctcacg ccaccgatat tacgagcaaa taaaaagcgg
     1381 catgcggctt cctgagcagg aagacgcatg ccgctttgct gttaatccag tctggtacta
     1441 ggacaggttc gctttgaaaa agctcgtggt tctcgtccag gcgtctttcg ccgcatcggg
     1501 gttataacgg tcggcgttgg tgttgttgtt gaaggcgtgt ttggcgccgt cgtacacttt
     1561 gcagtcaaag ctcttgccag ccttggccat tgccgctttc aagggctcaa caccgggcat
     1621 gatgttgggg tcatcgccag cgtagttgcc catcaccggg caaggaatat tggcgacgga
     1681 atcgagtgga tcaggattcc tgccgtagta aaccacagcg gcattgagat ctttgcattt
     1741 ggtcgcaaaa ttaagcgact ggccgccgcc ccagcaatat ccgaccacgc ccactttacc
     1801 gttgacgtag ttttgctttt tcatataggc caccgcggcc gtcaagtctt cggccgccat
     1861 ggcgccgtta attttttgaa tttctttggt ggcctcgtca ctgccattcc acttgtcact
     1921 gccaccgatg cggctcatca ggtcgacccc gatcgccgca aagccatctt tagcgaaccg
     1981 gcgggtgaca tcctttatgt gacccaccaa accgcggttt tcatggatca cgatcacggt
     2041 gccgagattc ttgccgtcct tgggccgtga aacgtaagcg tttacgtcgc cccccgggct
     2101 ggtgaacttt accatctcgg atgttaaacc gggatcgttc ggatcgacga tattttcaat
     2161 tgtttgagcc attgttcaaa acctccttca agtttctttc cgctcccagc tatagcgaaa
     2221 aagcaccgtt aaatctagtg ttatttcgtc ccgctaagtg actaattacg gcgccagaaa
     2281 cgacaacggc gccggttagg cgccgctgga tagtgattgg tcgtctggaa ccctcggcgg
     2341 gttagttagg ttgacgcagt actcgaagtc gaagtcgatt tgtcggtgct ggtggaggaa
     2401 ctgtcgccct tcttcgtttc actcttgctt tcactttttg attcactctt tgagtcggca
     2461 gaggacgacg atgatgatgc cggcttcgag gcgttatcgc tcttcgcctc gccgttttgc
     2521 cccttgcggg cgtagtccgt gacgtaccag ccggtgccct ttaattgaaa cgacgtgttg
     2581 gaaatcaact ttttgacctt gctcttgcac ttcggacact tagccagggg cttttccgtg
     2641 atcctttggg tgacctcaac gacgccgcat ttctgacagc tatactcgta aataggcatg
     2701 aaatatcctc aacttgcgca actgccaggt aaattaacca ctagcctgtc cgaagtcaat
     2761 tcgcgtccaa acggctgtct gcgaataatc caatcacgca ccctggtgga gcttgctgcg
     2821 atccctgccg tcggccagga tatgcgcacc gccaaaggcc tcggagcggt tgccgaaaag
     2881 caccaacggc tcggtccccg tattgacgat ttgataataa tccttggccg ctaaacagag
     2941 gacatcgtgc tgcttgatcg gcttctcctc gcccttgagg ccctttacta cgccgttgcc
     3001 ggagaaaacc aaaaaagttt caggactggc atgatagtgc atatccgtcc cgtcgccggg
     3061 aacaaaatat agcaaccacg accggaagtt atccgtaccg tagagatcga tcttgcgttt
     3121 ccccttggcc atctcttctt gcagatttaa gaattccata ggttcccctt tccgtggttt
     3181 caatttcatc actctatcct actggtcttg gcttgagcaa gcgcagtcgt cttgacagcc
     3241 cgtcgaccct gccatatgca aatgaggtct aacagcggca tcgacgggag gaagacgcaa
     3301 tgctcaagtt gaacgaaagc caggccaagg aatacatcgc tgaactggcg gacaagtgcg
     3361 accggcgctg gaaacagacg gtcgacaaaa cgaaattcat ggatgaacta aaagaaggca
     3421 agctgaaaaa agaaacgatt cagctttttt acaaaaattg gggcgccttc gtgccggtga
     3481 tcaactcggt ctataccgcg gcgttttata aacatctatg gttcttcgtc aagaacgtcg
     3541 atttgatgga agtttacacc cagaaggttc tcgacgagtt cggccatccg tcgccgcccg
     3601 gccacattca gatcctgatg agcaccggcg ccgctctcgg attgaccaaa gaagaggtgc
     3661 tgctcgcgcc catgctgcca caatgccgcg ccctgccgga ctttcatcgc accttgatca
     3721 acgacgggca gatccaagag tactggttct cggtcctgtg ggaacacccg tttggtgaat
     3781 cttgcttgga ctttttcaaa tcactgaccc gatactatgg cctgaccacc gccgaagcga
     3841 gttatttctc caaacatcac gaagccgaca ccatggacca cctcgatcgc atggcccatg
     3901 gcgcggtgac ccagcatgtg ctggtccgcc tgctccagga aggcatcaac gaacgccccg
     3961 gctactccgc agagtactgc gccgtaaccc cggcggactt gaacaaaatg ttcatggacg
     4021 gctgctatgc cgctacgcac tagccggcgg ctaggcgtat tcaaaatttc atattcgaaa
     4081 ttccacagtg gccggaaatc cgaaatatgg aatgtggaat atggaatttg aaattggtcg
     4141 cgcctatcag aagcgctagg agcaagaccc tagactccat tgatcaatga cttaggagcc
     4201 tgcaaaatgg catttagcga tctacgggct ttcatcgacg gcaccgcccg catcggcgaa
     4261 ctcaaactaa tcgacggcgc cgattggaat cttgaaattg gctgcttgac cgagctgatg
     4321 gccgagcagg aaggcccgct gttactcttt gacagcgttc ccggttatcc caagggcttt
     4381 cgcatcgccg ccaatgcgct cgccacggca cgccgtttcg ccctcgcctt ggggctgccg
     4441 acggatgcgc cgaaactgga aattctccgc gcctggcgca acaagattcg cgatctgaaa
     4501 cctttcgcgc cggtagaagt cgccaccgga ccggtgctgg aaaatatact ggaaggcgac
     4561 cggatcgatc taggaatttt cccgacgccc aagtggcacg agctcgacgg cggccgctac
     4621 atcggcaccg gcgacatggt ggtagtgaaa gaccccgaca gcggctggat caacgtcggc
     4681 acctatcgct cctgcattgc cggcaaagac cgtgtgacgc tgtggatcat tgagcagaag
     4741 catggcaagc agatcgcccg caaatactgg cacgcgggta aaccttgccc ggtcgccatc
     4801 gtgctcggct gcgagccggc gacttggatg gccgcgccgt cggcggccaa agccggcgtc
     4861 agcgaatatg aatacgccgg cgccctgcgc agcgcgccgc tcgaagtgat ccgcgctccc
     4921 tacaccggac tcccggtgcc ggcaacctcg gagatcgtcg tcgagggcga aatgccgccg
     4981 ccggaggagg aaagccatag cgaaggccct tttggcgaat ggcccggata ctacactcac
     5041 agcggtgaag agtgcgtcgt gcgggtgaaa agaatcctcc accgcaacga cccgatcctg
     5101 ctcggcaatc cgcccctgct gccgatcacc gaacgttacg gtatcccgct attcgcggcg
     5161 cgcatctggg accatctcga acaatcgggg gtgtcggacg tgcaaggcgt gtggtgtcat
     5221 tgtcacacgc tcatggtcgt cgtttcactc aaacagcgct tcgccggtca ggcgttgcaa
     5281 gcgctcaccg cggtcgccgg catgatgacc ggcgccagca tgtaccgcta ttacatcgcc
     5341 gtcgacgacg acatcgaccc ggtggactta aagcaagtca tctgggccat gtgcacccgg
     5401 gtcgacccgg ccgagtcggt gcaggtacta aaatcctgga cctccgacct tgacccgcgc
     5461 ctggcgccgg aaaaacgcga acgcggcgac ttcaccatgg gccgcatgct catcgatgcc
     5521 tgcaaaccgt ttcactggcg cgacaagttc gccgtggcga acaaattcag cgccgataaa
     5581 cgcgaagaaa tctgggaaaa atgggcgccg aagctgggac tgaaataaac gtccaaacga
     5641 tcccgtaggg acgctaagag acgaaggatc accatgatgg cgctgtggat tttactttta
     5701 ctgactgcac agttattcgt gccggacgcg caagccgccg gcgaagtctt agccgagatc
     5761 gacggcgttg ccattaccgc cgaggaggta gagaagccgc ttgtgtctca gttgagcagg
     5821 cttgacgagc agatctatgc cttaaagtct cagaagcttg acgcgctgat tgacgaaaag
     5881 ctgctggcaa agcaagcggt cgaacgaatg ctcacggtag cggcgctgct tgacgccgaa
     5941 gtaaccagga aggtcggctc gatcaccgag caggaaatcg acgaattcta ccagcgtaat
     6001 aaagcgcaaa ttaacgcaga gctggcgcag gtccgggagc aaattagggt ctatttacaa
     6061 aaccaacaat tggcggccaa gcgcgaagag tttctcaaat ccctgcgctc gcaagttaaa
     6121 gtcatcgtgc atttaaaacc gccgcaagtt catcgggtcg aggtgtcggt ccgaggcgcg
     6181 ccgttcaaag gaggcgagca ggcgccggtg acgattgtcg aattttccga ctttcactgt
     6241 cccttctgca cgcgcgtgac ttcgacgctc aagcaacttg agtcaatgta cggtgaaaaa
     6301 atcaagctgg tctttcgcga cttcccgatc gaggctctgc atcccggagc gac
//
LOCUS       DASQKS010000210         5362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1367239, whole genome shotgun sequence.
ACCESSION   DASQKS010000210 DASQKS010000000
VERSION     DASQKS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367239"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..908
                     /locus_tag="VE170_05485"
     CDS             447..908
                     /locus_tag="VE170_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ14921.1"
                     /translation="MEIENKLKALGIELPNPPTPAANYIGYVRVGNLLFIGGNIGRIN
                     GAIRYRGKVGAEISVEQAYDAARNCALNHLAIMKSALGDLDKVARIVKVLGYVNVAPG
                     FVDMPKVVNGESDLLVEVFGERGRHTRAAIGVASLSQDAPVENEITVQVYD"
     gene            complement(949..1116)
                     /locus_tag="VE170_05490"
     CDS             complement(949..1116)
                     /locus_tag="VE170_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14922.1"
                     /translation="MIEYQGIMRPQPSNPNAMERQRAIADKATATLTDHLTYRLRQLG
                     FIEEALSTAMQ"
     gene            complement(1355..1681)
                     /locus_tag="VE170_05495"
     CDS             complement(1355..1681)
                     /locus_tag="VE170_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14923.1"
                     /translation="MEKFGYLLSEIPVLFLIIWFAVRFVQYRRYRNKQPIFSEKDVAL
                     LYPPTKFDLRSPMSRLRLARCILLATAAFYVEFLILAPLGAAILCASLMLTTAALVQN
                     FILSPS"
     gene            1879..2982
                     /locus_tag="VE170_05500"
     CDS             1879..2982
                     /locus_tag="VE170_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ14924.1"
                     /translation="MNFVSSLSWHDWPVRLAFVVALLCIGATVPGGAASVEEVALAKA
                     ANRQTLLEEGAKKEGKLLWYTTLIVNQALKPIKEVFEKKYPYVQVEYHRADSEALAQR
                     MLAEYQAKRYDIDVLDGTSTIVMLKKAGYIQRFATPQLRDYPERLKEPQGFWAVPNLY
                     FMTLGYNTKLVAAHEVPKSLNDLLNAKWKGKMIWNTSGGSGAPVFLGNTLITLGEQKG
                     MGYLKQLAGQNLAKSTASARAVMDMVIAGEYAIGINMFNYHAVISRAAGAPVDWQAIE
                     PVPALVKTLGLAKHAPHPHASMLFIDFLLSKDGQKILQEADYIPAHPDVPAKVADLKA
                     GGGKFSKFNFIAPEILFEKEEKWVELFEKMFFK"
     gene            complement(3099..3944)
                     /locus_tag="VE170_05505"
     CDS             complement(3099..3944)
                     /locus_tag="VE170_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HYJ14925.1"
                     /translation="MKTIALLHPGNMGSTIGACAATSGARVLWASAQRSAASRKRAEQ
                     AGLVDAGDLAAAVRQSDIVLSVCPPEFATEIAATVAAQRFQGIYVDANAVSRETAEEI
                     AKMVTKSGASFVDGGIIGSPVKQAGTTRMYLSGEKAAEVAQLFSVSMLDARSIGSLPG
                     EASALKVVYAAWTKGTDALILAIRALAAHEGVDHALLEEWKISQPALENKCLRAAAVA
                     VPKMWRYVGEMREIAEAFQAAGLPGGFHQAAAEVSERLASFKDQTEPPLTVKTVLEKL
                     LSPAT"
     gene            4041..5144
                     /locus_tag="VE170_05510"
     CDS             4041..5144
                     /locus_tag="VE170_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ14926.1"
                     /translation="MIILSNEEIESFLSIKTCIDALEKAYASWDNGTAINRPRTDLVM
                     PSATEAGVYAFKSMEAGLYDPPIVAMRINSDIIRWSQQGERTIKTKVPAAPGDKYVGL
                     VMLFSTITGEPLAMFPDGVVQRMRVASSSALAARYLAREDSSTMALFGSGWQAGSHVP
                     AMCAVRPLKRINVYSPTKANREAFVKEIQPKVSAELRAVDSPEEAIRDADIIAATTNS
                     LSRVVSADWVKAGLHLTCVRVPELGDDTIRKVDRLVIHAHQHAPANYIAGYGEEGIHA
                     HDAIDVIKKGPAHAHEINVEHPFWLAAPALKDLVTGKAQGRASAQESTCFLNNIGIGL
                     QFAAVGAAVYSEAIAKGIGRELPTDWFLESVHP"
BASE COUNT         1177 a         1437 c         1578 g         1170 t
ORIGIN      
        1 aggcgacgat atcttccagg agatcgagta aggcttcggg gtcgtacggt gggtgctcgg
       61 cgatgctcat gatctcgttc tagcgcaaaa gtcgtgcaac accatcggca actggattag
      121 cggcattggg gtttaccctt cctgacatat tgcggaactc tggatagcgc gtagtcgaat
      181 tagtggggtt ttcggtcagc ggccgacgca ttctacgacg cacaactctg gttgatcctt
      241 gaccaaattg tttcggcctt ggttgtctgt actggcgtga tttgtcggcc tgcgagcacc
      301 aatggctttg cctttggctc ggagagcctg agcgattgcc gcgattggtt atcgtcccgg
      361 gaaggttggg gtaggtagtt ttgaccccga tcgttgatgg gtatggtatc gagccaagcc
      421 acattgatca aataaaggag gcgttgatgg aaatcgaaaa caagctcaaa gccctcggta
      481 tcgaactgcc caatccaccg acaccggcgg ccaactacat cggctacgtc cgggttggta
      541 atctgctgtt catcggcggc aatatcggcc gcatcaacgg cgcgatcaga tatcgtggta
      601 aagtcggtgc tgagatcagc gtcgaacaag cttacgacgc tgcgcgcaac tgcgctctca
      661 accatctggc gattatgaag tcagcattgg gtgatttgga caaagtcgcg cgcatcgtca
      721 aagttctcgg ctatgtcaac gtcgcccccg gctttgtcga tatgcctaaa gtcgtcaatg
      781 gcgaatcaga tcttctggta gaagtgtttg gcgaaagggg ccggcacaca cgcgccgcca
      841 tcggggtggc gtcgttaagt caggatgcgc cagtggaaaa tgaaattact gtccaggttt
      901 acgactgaga tcgagtgtga atttcatcga tcaacaactc gtgttcgctc actgcattgc
      961 cgtgctcagc gcttcctcaa tgaatcccag ttggcgcaac ctgtaagtta ggtggtcggt
     1021 caatgttgcg gtggccttgt cggctatggc gcgttgtcgt tccattgcat tgggattgga
     1081 cggttgtggg cgcatgatgc cttgatactc gatcacgtcc gacgcgctaa tcgcgaagtc
     1141 gtcgactatt attcaagtcg gccgtggcag ttgccgacgg ccgatttgcg ccaccggtcg
     1201 cacactggtt tgcgctcaga cggctagtag caatagacaa acggatcggg taacccaagt
     1261 gatttggcta gagtaacggt attttgctcg cggcatgaac ggatctgccg aagcggcatg
     1321 aacaggcaag caaatatcgg atcgcctgtg agaatcagct gggactcaga ataaaatttt
     1381 gcaccagcgc ggccgttgtc aacatcaaag aagcgcacaa gatcgccgca cccaacggcg
     1441 ccaatatcag aaactccacg taaaatgctg cggtggcgag caagatgcaa cgcgcgagtc
     1501 tgaggcgcga catcggcgag cgcaggtcga acttggttgg tggatatagc aaggcgacgt
     1561 ctttctcgga aaagatcggt tgcttatttc ggtaacggcg gtactgcaca aaccggacgg
     1621 caaaccagat tatcagaaat aagacgggta tttccgaaag taagtaacca aacttttcca
     1681 tatctactcc catcccttga tcgcgaagat ttccacgccg gtgtcatgat gccgttacct
     1741 ataagaagtg gcgggtgatg tcaaaacctg gtaatgcggc gcatgctaga ggtcaatctg
     1801 aattaatcgt tggtttccgg gagttgcagc gctatatttg gcgcgattgg taaattagtg
     1861 agatttgcgg aggcaacagt gaactttgtg tcatcgctgt cctggcacga ttggcccgtt
     1921 cgattagctt ttgtcgtcgc attgctatgt ataggcgcga ccgttccggg cggcgccgcc
     1981 agcgtggagg aagtcgctct ggcgaaggct gccaaccgcc agaccctgct cgaagaaggg
     2041 gcgaaaaaag aaggcaagct gctctggtat acgacgctga tcgtcaacca agcgctcaag
     2101 ccgattaagg aagtattcga aaaaaaatat ccttatgtcc aggtcgaata tcaccgcgcc
     2161 gattccgagg ccttggcgca gcgcatgttg gccgagtatc aggccaagcg ttacgacatc
     2221 gatgtgctcg acggcacttc gacaatcgtt atgctgaaga aggcgggtta tattcagcgc
     2281 ttcgcgacgc cgcagctgcg cgattatccg gagcgtctca aggaaccgca gggtttttgg
     2341 gcggtgccga atctatattt catgaccctc ggctataata ccaagctcgt cgctgctcac
     2401 gaagtgccga agtcgctaaa cgatctgctc aatgccaaat ggaaaggcaa aatgatctgg
     2461 aataccagcg gcgggtccgg cgcgccggta tttctcggca acacattgat cacgttgggc
     2521 gagcagaaag gcatgggcta tttgaaacag ctggcggggc agaacctcgc taagagcacg
     2581 gccagtgcac gagcggtgat ggatatggtc atcgccggcg agtacgccat cggcatcaat
     2641 atgttcaact accacgcggt tatcagccgc gccgccggcg cgccggtgga ttggcaggcg
     2701 attgaaccgg tgcctgcact ggtgaaaacc ctcggactgg cgaagcacgc gccgcatccc
     2761 catgcgagca tgttgtttat cgattttctg ctctcgaaag acggccaaaa aattcttcaa
     2821 gaagcggact atataccggc acatcccgat gtgccggcga aagtcgccga tctaaaggcc
     2881 ggcggcggca agttttccaa gttcaacttc atcgcgccgg aaatactctt cgagaaagaa
     2941 gagaaatggg tcgagctgtt cgagaagatg ttctttaaat aatcgacgaa ggattaagct
     3001 cgcgcgcagt gtcggcaatg ccgctgggtc attttgagcc cgagcctagg atcaacctct
     3061 ctccggcatc gagacaggca agacagcgcg atgcgagact aagttgccgg tgacagcagc
     3121 ttttcaagta ccgttttcac cgtaagcggc ggctcggttt gatctttgaa ggaggcgagc
     3181 cgttcgctga cttcagcggc ggcctggtga aaaccgcccg gcaagccggc ggcttggaag
     3241 gcttcggcga tttctctcat ttcgccaacg tagcgccaca tttttggcac ggcgacggcg
     3301 gcggcgcgca ggcatttatt ttccagcgcc ggttgggaga ttttccactc ttcgagcaat
     3361 gcgtgatcca cgccttcatg ggcggcgagc gcgcgaatgg cgaggattaa agcgtcggtg
     3421 cctttggtcc aggcggcata gacgactttc aacgcagagg cctcgccagg cagggagccg
     3481 atgctgcgcg catccaacat gctgacggaa aacaattgag caacctccgc ggctttttct
     3541 cccgagagat acattctagt cgtgccggct tgcttgaccg gcgagccgat gatgccgccg
     3601 tcgacgaagc tggcgccaga tttggtaacc atcttggcta tttcttccgc cgtctcccgc
     3661 gacacggcgt tggcatcgac atagattcct tggaagcgtt gcgcggcgac ggtcgcggcg
     3721 atttccgtag cgaactccgg cggacagacc gacagcacga tatcgctttg gcgcacggcc
     3781 gcggccagat cgccggcatc gaccagaccg gcttgttcgg cgcgcttgcg tgatgcggcg
     3841 cttcgctgtg ccgatgccca gagcacccgg gcgccgctgg tcgcagcgca cgcgccgatg
     3901 gttgaaccca tgttgccggg atggagcaat gcgatggttt tcatgattat cctttcctga
     3961 ttgcgaggct aatcgagttg tgtgtcttta gtcaatgatc ttggcttgac gatagtagac
     4021 cgcgctgata tttcaactcc atgattatac tcagcaatga agaaatcgaa tcgtttctgt
     4081 cgatcaaaac ctgcatcgac gcgctcgaga aagcctacgc cagttgggac aatgggactg
     4141 cgatcaaccg gccgcgcacc gatctggtca tgccgtcggc gaccgaagcc ggcgtctacg
     4201 cttttaaatc catggaagcc ggcttgtacg atccgccgat cgtcgccatg cgcatcaatt
     4261 cggatattat ccgctggagc caacagggcg agcgaacgat caaaaccaaa gtgccggcgg
     4321 cgccgggcga caaatatgtc ggcctggtga tgttgttctc gacgatcacc ggagaaccgc
     4381 tggcgatgtt ccctgacggc gtggtgcagc gcatgcgcgt ggcgtcgagc agcgcgctcg
     4441 cggcgcgcta tctcgctcgc gaggactcca gcacgatggc gctgttcggt tccggttggc
     4501 aggctggcag tcacgtgccg gcgatgtgcg ccgtgcggcc gctcaagcgc atcaacgttt
     4561 atagtccgac caaggccaac cgcgaagcgt ttgtcaaaga aatccaaccc aaggtgagcg
     4621 ccgagctgcg cgcggtggat tcgccggagg aggcgatccg cgatgccgat atcatcgcgg
     4681 cgacgaccaa ctcgctttcc cgcgtggtca gcgccgattg ggtcaaagct ggactgcatt
     4741 tgacctgcgt gcgcgtgccc gagctgggcg acgatacgat tcgcaaagtc gaccgcttgg
     4801 tgattcacgc gcatcagcac gcgccggcga attatatcgc cggttatggc gaagaaggga
     4861 ttcacgccca tgacgccatc gacgtaatca aaaagggtcc ggctcatgcc cacgaaatca
     4921 acgtcgaaca tccgttctgg ctggccgcgc cggcgctgaa ggatctggtc actggtaaag
     4981 cccaaggacg ggccagtgct caggagtcga cctgttttct caacaacatc ggcatcggtt
     5041 tgcaattcgc cgccgtcggc gccgctgtct atagcgaagc catcgccaaa ggcattgggc
     5101 gcgagctgcc gacggattgg tttctggaaa gcgtgcatcc ctagggcggc ggcttggcaa
     5161 tcggaagttt agccgccaac aacgcagcgg cctcacgaat acatatcaat gcctaaatcg
     5221 aagtcatctt gatattaacc ttgatttgat tgtcgcctaa tactggatga gagtctcgcc
     5281 gcgctcgaaa tgacagctgc ctgccgttat tgtccgcgca gggtgggttc gtgccatctg
     5341 aacatccctc cgattgactc gc
//
LOCUS       DASQKS010000211         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4728670, whole genome shotgun sequence.
ACCESSION   DASQKS010000211 DASQKS010000000
VERSION     DASQKS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4728670"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VE170_05515"
     CDS             complement(<1..618)
                     /locus_tag="VE170_05515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ14927.1"
                     /translation="MTHNRIRHTFGGTLQSEVDVKFGYYILNTYVPELDGGSREIYSK
                     WLEQMDAAEALGFDSLWVTEHHFRHFGGQMPSPSVILAAAAQRTKKLRLGAAVSLLPM
                     HNPLRIAEEFAMVDQLSAGRLCFGAGRGMHPTEYAVFGADWSNAQKRLPEALDIVRRA
                     WSGEEFEWHSEHYKFPKITVYPQPYQRPHPPIYITANRDPESFHLV"
     gene            738..1607
                     /locus_tag="VE170_05520"
     CDS             738..1607
                     /locus_tag="VE170_05520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ14928.1"
                     /translation="MIRITSAGRLLLALFCLASSVAARAQELEKLPYVPTPQIVVDEM
                     LKLAAVNEKDFVVDLGSGDGRMIITAARTFKANGLGVDIDATLVELATRHARNEAVSD
                     RAKFIEQDMFKADISKATVVTLYVLPDFMEKLRPKLMRELKPGARIVAHDYYMSEWRP
                     DRFAEMDVPEKKAANGTEKAYLYLWTVPAIVQGDWRMNWDFGDGKPQLIVLAFNQRYQ
                     MINASAASKDGEMKIANASLKGDDIDFFLTVGAANYRFTGKVQGERMSGNAVTATGGK
                     PIPWSAAKMPPEK"
     gene            1729..2787
                     /locus_tag="VE170_05525"
     CDS             1729..2787
                     /locus_tag="VE170_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14929.1"
                     /translation="MNAPASSYTPEVVLAGEKIISADSHIQEPEDLWVDQLPAALKDR
                     YPKFPKRNSPGEKPGGYDPKARISEMETDGVSAEVLYPTLGLRLFAMEDAEAQEACFQ
                     IANDWMIDYCKVTPGRLIGIPMISLYNIKNAIKELERCKKAGMVGCLIWQVPPDHLPF
                     SSDYYDPFWEAAGEMNMPVNLHILTGFNYSRFERKGLDTYKTAVNDKVNDAANTLFDL
                     IFTGVLSRFPKLKLVYVENECAWLPFYSHEWDKYYTRFKTKVPLPFMKKLPGEYIDEQ
                     VYATFFSDPCGGQLMNRFGQDTFMWSNDYPHAASPWPHSRKVIAEELGHLPKDVLRKV
                     VRENVIKLYNLKIDGINN"
     gene            2905..>3214
                     /locus_tag="VE170_05530"
     CDS             2905..>3214
                     /locus_tag="VE170_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14930.1"
                     /translation="MQKSATAAVFKNYDQPKTQLFSLKTPYMEQGRVTQLVAETPNLW
                     IHTKVNAEGGENEIHKHLGEDHAFIVLEGQMSVFDEQGGEIEVKQYQGVMIPKGAYYR
                     Y"
BASE COUNT          783 a          933 c          875 g          623 t
ORIGIN      
        1 gaccagatga aaactttccg gatcgcggtt ggcggtgatg tagatcggcg ggtggggccg
       61 ctgataaggc tgcgggtaga ctgtgatctt ggggaatttg taatgctcgc tgtgccactc
      121 gaactcttcg ccgctccacg cccgcctgac gatatcgaga gcctcgggca atcgcttctg
      181 cgcattgctc caatcggcgc cgaacaccgc gtattcagtc ggatgcatgc cgcgtccggc
      241 gccgaaacag agccgtcctg cggaaagctg atcgaccatg gcgaactctt cagcgatgcg
      301 cagcgggttg tgcatcggca gcagcgacac cgccgcgccc aagcgaagct tcttggtgcg
      361 ctgcgccgcc gccgcgagaa tcaccgacgg gctcggcatc tgcccgccaa aatggcggaa
      421 gtgatgctcg gtcacccaca acgaatcgaa acccagcgct tccgcggcgt ccatctgctc
      481 gagccacttg gaataaatct cccggctacc gccatccaac tcgggaacgt aagtattgag
      541 aatgtaataa ccgaacttca catcgacctc gctttgcaac gtgccaccga aggtatggcg
      601 aattcgattg tgggtcaagc aaagcccgca ggttatcatt tggcagtaat ccggcaatgc
      661 ctgcacatcg ccactgctat ttgcaatcgc gcggtaaatt gggattatag aacgataatg
      721 atcctaagga gacccgcatg attagaatca ccagcgccgg ccgtctgttg ttggcgcttt
      781 tttgcttggc gtcatcggtc gcagcccggg ctcaagaact tgagaaactg ccctacgtgc
      841 cgacaccgca gattgtcgtc gacgaaatgt tgaaattggc cgccgtcaac gagaaagatt
      901 tcgtcgtcga cctgggctcc ggcgatggcc gcatgatcat caccgcggcg cgcaccttca
      961 aagccaacgg gctcggcgtc gacatcgatg ccacgctcgt cgaactcgcc actcgtcacg
     1021 cgcgcaacga agcggtcagc gaccgcgcca agttcatcga gcaggatatg ttcaaggccg
     1081 acattagcaa agccacggtg gtgacgcttt atgtcttgcc cgatttcatg gaaaagctcc
     1141 gtcccaaatt gatgcgcgag ttaaaacccg gcgcgcgcat cgtcgctcat gattattaca
     1201 tgagcgagtg gcgccccgat cgcttcgcgg aaatggacgt gccggaaaaa aaggccgcca
     1261 acggcacgga aaaagcctat ctctatctgt ggaccgttcc ggcaattgtc cagggcgact
     1321 ggcgcatgaa ctgggatttc ggcgatggca aaccgcagtt gatcgtcctg gccttcaatc
     1381 agagatacca aatgatcaat gcttcagccg ccagcaagga cggcgaaatg aagatcgcca
     1441 acgcttcgct caaaggcgac gatatcgact tcttccttac cgtcggcgcg gcgaattacc
     1501 gcttcaccgg caaagtccag ggcgagcgca tgagcggcaa cgccgtcacc gccaccggtg
     1561 gcaagccgat cccgtggagt gccgccaaga tgccgccgga aaagtgagtt tgacttcttg
     1621 gcttgggtct cgtagggaaa gggaagttgt cgaaccgcct tgggccttga cggatcattt
     1681 aatcaccgac aaaaaaaata aacccagatc gaaagcgagg gaaatctcat gaacgcaccc
     1741 gcatcctcat acacccctga agtggtattg gccggcgaga aaatcatttc agccgactcc
     1801 cacatccagg aaccggaaga tttatgggtc gatcaactgc ctgccgcact gaaagaccgc
     1861 tatcctaaat ttcccaagcg caactcgccc ggcgaaaaac ctggcggtta cgaccccaaa
     1921 gcgcgcatca gcgagatgga aaccgacggc gtgtcggccg aggtgctcta tccgacgctg
     1981 gggctgcgct tgttcgccat ggaagacgcc gaagcccagg aagcctgctt ccaaatcgcc
     2041 aacgactgga tgatcgacta ttgcaaagtg acgccgggcc ggctgatcgg cattccgatg
     2101 atctcgctgt acaatatcaa gaacgcgatc aaggaactcg agcgttgcaa gaaagccggc
     2161 atggtcggct gcttgatctg gcaggtaccg ccggatcatc tgccgttttc cagcgactac
     2221 tacgatccct tctgggaagc cgccggcgag atgaatatgc cggtgaattt gcatattctc
     2281 accggtttca actacagccg cttcgagcgc aaagggctcg acacctacaa gaccgccgtc
     2341 aatgacaaag tcaacgacgc ggcgaacacg ttgttcgatc tgatcttcac cggcgtgttg
     2401 tcgcgtttcc caaaactgaa gctggtctac gtcgaaaatg aatgcgcctg gctgcccttc
     2461 tattcacacg agtgggacaa atattacacc cggttcaaaa ccaaagtgcc gctgccgttc
     2521 atgaaaaaac tccccggcga atatatcgat gaacaggtgt acgcgacctt ttttagcgat
     2581 ccatgcggcg gccagctgat gaatcgtttc ggccaagaca ctttcatgtg gtcgaacgat
     2641 tacccccacg ccgcctcgcc ctggccgcac tcgcgcaaag tgatcgccga agagttgggc
     2701 cacttgccca aagacgtgct gcgcaaggtc gtgcgcgaga acgtcatcaa gctctacaac
     2761 ctgaagatcg acggcatcaa caattgaccc gcttgccccc aagcgggtca tcccgaacgg
     2821 aggtgaggga tctagatttc tcgcggagtc tatcctgagc gctgccgaag agctcgaaat
     2881 gacaaaataa cacggagaat cgccatgcag aaaagcgcca ccgcggcggt ttttaaaaac
     2941 tacgatcaac cgaagactca gctgttttcg ctcaagacgc cgtatatgga acaaggccga
     3001 gtcacccagc tggtcgcgga aacgccgaac ctatggattc acacgaaggt caatgccgaa
     3061 ggcggcgaaa acgaaatcca taaacacctg ggcgaagacc atgcgttcat cgtcctcgaa
     3121 ggccaaatga gcgtcttcga tgaacagggc ggcgagatcg aagtgaagca atatcagggc
     3181 gtgatgatcc ccaagggcgc ttactatcga tatt
//
LOCUS       DASQKS010000212         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_109028, whole genome shotgun sequence.
ACCESSION   DASQKS010000212 DASQKS010000000
VERSION     DASQKS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109028"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VE170_05535"
     CDS             <1..1077
                     /locus_tag="VE170_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14931.1"
                     /translation="DGMYGGVNIVDSETISWLHSAIVQRGADATVLWCDIPYRPTVLW
                     GEGADPGKAWRVPKIVAAAMKGADCIISNAVDLTFEEELRETADILEEHNIPYARNMA
                     TTGSLLTSNWGLTPYELVSEIRVQANALAVPGAKWVMTHPNGSHIEGYVVKPEEMDDY
                     AHYRDIGVYRPFPEGVWSSIRARDAEGVGIINEIGVIWARHIGVPCPFKQPVKVFIEK
                     GQIKKFEGGAEAETLTRFYQFISKYLGDAAYEVRGFHGGVHPSAILEPHQCPNAAYRH
                     FIEHHHWSSFHFHMGNSRRSKTFPYLMHISAELRGGNLKIGENYVYKDGKISSADHPK
                     VRAIAAKYKDRPGLEPEKWLQTPR"
     gene            1139..2284
                     /locus_tag="VE170_05540"
     CDS             1139..2284
                     /locus_tag="VE170_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14932.1"
                     /translation="MATKYSLFSADSHLEIAPSRWTDRIPVKYRDRAPRLIKLANGGD
                     GIIIEGRPLYVVGLAIAGKPYERHELRGVSYEGSEGAGSPEQRLKEQERDGVDGEILY
                     TSAGNLSFWRGIKNDDAYLATVHAYNTFLAEEYCAANRDRLIALGALPTYSVDAALKE
                     LEYCAKAGLKGMMLNTFPSGKSYPTLEDDRFYAAALDLNMPLSVHVAMGLPDGPLFQY
                     AKDPGEVAFGGDPIRVLTRFGGNSGLNAVQLLLSGVFERLPKLKIYWAETQMGWLPYY
                     YDQLDDVYKRSKHWMQRYFDLKPLAKEPSAYFREHFYWGFIYDRVGLRMRYDVGVDKI
                     MWGNDFPHSAGDWPNSKRVIADIFSGVPDDEKQKMLVDNAVNFFHLN"
     gene            2366..3421
                     /locus_tag="VE170_05545"
     CDS             2366..3421
                     /locus_tag="VE170_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14933.1"
                     /translation="MADSISDVRSKRLETLNAKVQQSSLRGAWQRERGAAQQEEIRPS
                     VWRWSEMAPLLLEAGQLAPLDEVMRMRTLHMTNSADTLPLGASRTFAAMLQHVNGGEV
                     TDSHRHTATSVYLAIQGSGIYTTAEGEQQFMEPGDLLTQPSWTWHGTTNTGSEPAIWF
                     TAMDTALMQYLDNWENQRYAESFSQPITKPDGFHMKQLGPLRAQGMLDGPGPFPVKYR
                     WAETYGALKDLALSGERDPFDGAVLEFRNPQDGGPTTATMSCRMQLLGPGEQTQPHRH
                     TGNTVYHVVRGQGVAMIGKQRAVASDLTWQERDCFNVPTWHWHRFLNTSTSEDAILFS
                     VSDRPLFDALRLYREEF"
     gene            3493..>4149
                     /locus_tag="VE170_05550"
     CDS             3493..>4149
                     /locus_tag="VE170_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ14934.1"
                     /translation="MAEQLRIICRACQNGSAADAQFCDACGAPLDASCRQCGESNRSG
                     AKFCKKCGARLGEVAGAAAPAAAAAAVTTDNVADRMLAARRFMEDERKRVTVLFADIR
                     GSTSFIEKLDPEEVRKHFDPVLRVMMAAVHRYDGTVNQVLGDGIMALFGAPLAHEDHA
                     LRACYAALAMQAELRRGGAADGPSPQPAPLQIGIGINSGEVVVRSLTNDLNIDYSALG
                     Q"
BASE COUNT          864 a         1169 c         1294 g          822 t
ORIGIN      
        1 gacggcatgt atgggggcgt caatatcgtc gactcggaaa caatctcgtg gctgcactcg
       61 gcgatcgtgc agcgcggcgc cgatgcgacg gtgctgtggt gtgatattcc ttatcggccg
      121 accgtgttgt ggggcgaggg cgccgatccc ggcaaagcgt ggcgcgtgcc aaagatcgtc
      181 gccgccgcga tgaagggcgc tgactgcatc atcagcaacg ccgtcgatct gaccttcgaa
      241 gaagagctgc gcgagaccgc cgatattctc gaagagcaca acattcccta cgcgcgcaac
      301 atggcgacga ccggctctct gttgacgagc aactggggct tgacgcccta cgaattggtc
      361 tctgagattc gtgtccaagc taacgcgctg gcggtgccgg gcgccaagtg ggtgatgacc
      421 catcccaatg gctcgcatat cgaaggttat gtggtgaagc cggaagaaat ggacgactac
      481 gcgcattacc gcgacatcgg cgtgtaccgg ccgtttcccg agggcgtctg gtcgtcgatc
      541 cgggcgcgtg acgcggaggg cgtcggcatc atcaacgaga tcggcgtgat ctgggcgcgc
      601 catatcggcg tgccttgtcc gttcaaacag ccggtgaaag tcttcatcga aaaaggccaa
      661 atcaaaaagt tcgaaggcgg ggccgaagcc gaaactttga cccgcttcta ccaatttatc
      721 tcaaagtacc taggcgatgc cgcctacgaa gtgcgcggtt ttcacggcgg cgtccatccg
      781 tcggcgatct tggagccgca ccagtgtccc aacgcggcgt atcgccactt catcgagcat
      841 catcattgga gcagtttcca tttccacatg ggcaacagcc ggcgcagcaa gacgttccca
      901 tacctaatgc atatctccgc cgaactgcgc ggcggcaatc tcaagatcgg cgagaattac
      961 gtctacaagg acgggaaaat tagcagcgcg gaccatccga aagtgcgcgc gatcgccgcg
     1021 aagtataaag atcggccggg tctggagccg gagaaatggc tgcagacgcc gagataggct
     1081 ggttcaaggt tcaaggttca aaggttcaag gattatcgga aggatgggag agcgtttcat
     1141 ggcaaccaag tacagtttgt tctcagccga ctcacattta gaaattgccc cgtcgcggtg
     1201 gactgatcgc ataccggtta aataccgcga ccgcgcgccc aggctgatca aactcgccaa
     1261 cggtggggac ggcatcatca tcgaagggcg gccgctgtac gtggtcggct tggcgattgc
     1321 cggcaagccc tatgagcggc atgagcttag gggagttagc tacgaaggtt cagagggggc
     1381 ggggtcgcca gagcaaaggt tgaaagagca ggaacgggac ggcgtcgacg gtgagattct
     1441 ttacacatcg gccggcaacc tgagtttttg gcgcggcatc aagaatgacg acgcctatct
     1501 ggcaaccgtt cacgcctaca ataccttcct ggccgaagag tactgcgccg cgaatcgcga
     1561 tcggttgatt gcgttgggcg cactgccgac ctattccgtc gatgctgcgc ttaaggaatt
     1621 ggagtactgc gccaaggctg gactgaaagg catgatgctc aacacgtttc cgagcggcaa
     1681 gtcctatccc acgttggaag acgaccgatt ttacgccgcg gcgctggatc tgaacatgcc
     1741 gctgtcggtg cacgtggcga tgggactgcc ggacgggccg ctgttccagt acgccaaaga
     1801 cccaggggag gttgccttcg gcggcgatcc gatccgcgta ctgacgcgct tcggcggcaa
     1861 tagcggcttg aatgccgtgc agttattgct ctccggtgtc ttcgagcgct tgccgaaact
     1921 aaaaatctac tgggcagaaa cccagatggg ctggctgccc tattattacg accagctcga
     1981 cgacgtgtac aaacgcagca agcactggat gcaacgttac ttcgacttga aaccattggc
     2041 caaagagccg agcgcctatt tccgcgagca tttttactgg ggctttattt acgatcgcgt
     2101 tggcctgcgg atgcgctacg atgtcggcgt cgataagatc atgtggggca atgatttccc
     2161 gcattcggcc ggcgactggc ccaactccaa gcgggtcatc gccgacatct tttccggcgt
     2221 gccggacgac gagaagcaga aaatgctcgt cgacaacgcg gtgaactttt ttcatttgaa
     2281 ttaacggcga atctcgcgca gcaggcgatg ggttcgggta ggggcgaaaa atctttcgcc
     2341 cctacgtttt tgcggggagt ctgtcatggc tgatagcatt tccgatgtgc gttccaaacg
     2401 actcgaaacg ttgaacgcca aagtgcagca aagctccctg cgcggcgcat ggcagcgcga
     2461 acggggcgcg gcgcagcagg aagaaattcg cccatcggta tggcgctgga gcgagatggc
     2521 gccgctgttg ctcgaagccg ggcaactggc gccgctcgac gaagtcatgc gcatgcgcac
     2581 cctgcacatg accaactctg ccgataccct tccgctcggg gcgtcgcgca ctttcgccgc
     2641 catgttgcag catgtcaacg gcggtgaagt gaccgattcg catcgccata cggcgacttc
     2701 cgtctacttg gcgattcaag gcagcggcat ttacaccaca gcggaaggcg agcagcagtt
     2761 catggagccg ggcgatttac tcacccagcc gagttggact tggcatggca cgaccaatac
     2821 cggatcggag ccggcgattt ggttcaccgc catggacacc gcattgatgc agtatctcga
     2881 taattgggaa aaccagcgct atgcagaaag tttttcgcag ccgatcacca aacctgacgg
     2941 ctttcacatg aagcaactgg gaccgctgcg ggcgcagggc atgctcgatg ggccggggcc
     3001 gtttccggtg aaataccgct gggcggaaac ttatggcgca ttgaaagatc tggcgctgtc
     3061 gggagagcgc gatccattcg acggcgccgt gttggagttt cgtaatccgc aggacggtgg
     3121 tccgaccacg gcgacgatga gctgccgcat gcaactgctc ggtcccggcg aacagacgca
     3181 gccgcatcgc cacaccggca ataccgttta tcatgtggtg cgcggtcagg gtgttgccat
     3241 gatcggcaaa cagcgcgccg ttgccagcga cttgacgtgg caggagcgcg attgcttcaa
     3301 tgtgccgacg tggcactggc accgttttct caacacatcc acttcggagg acgcgattct
     3361 tttctccgtg tccgaccggc cgctgttcga tgccttgcgg ctgtatcgcg aagaattcta
     3421 aattcgcggc gaatttctta atcccggcca atctctttgc gccgtcgctg gaattgcgtt
     3481 aaatacgcaa agatggcgga gcagctaaga ataatttgcc gcgcttgtca gaatggcagc
     3541 gcggcggatg cgcagttttg cgacgcatgc ggcgcgccgc tcgatgccag ctgccggcag
     3601 tgcggtgaaa gcaatcgcag cggcgcgaaa ttctgcaaaa agtgcggtgc ccggcttggt
     3661 gaggtcgccg gcgcggcggc gccggctgct gcggctgcag cggtgacaac cgataacgtc
     3721 gcggaccgca tgcttgcggc gcgccgcttc atggaggacg aacgtaagag agtgacggtc
     3781 ttgttcgccg acatccgcgg ttcgactagt ttcatcgaga aactcgatcc ggaggaggtg
     3841 cgtaaacatt tcgatccggt gctgcgggtc atgatggccg cggtgcatcg ttacgacggc
     3901 acggtcaatc aagttttggg cgatggcatc atggcgctgt ttggcgcgcc cttggcgcac
     3961 gaagatcacg ccctgcgggc gtgctacgcc gcgttggcga tgcaagcgga attgcgccgg
     4021 ggtggcgccg cggacggccc ttcgccccaa ccggcgccgc tgcagatcgg catcggcatc
     4081 aactccgggg aagtggtcgt gcgctcgctg accaatgatc tcaacatcga ctactcggcg
     4141 ctcgggcaa
//
LOCUS       DASQKS010000213         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_968743, whole genome shotgun sequence.
ACCESSION   DASQKS010000213 DASQKS010000000
VERSION     DASQKS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968743"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..594)
                     /locus_tag="VE170_05555"
     CDS             complement(127..594)
                     /locus_tag="VE170_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HYJ14935.1"
                     /translation="MGCWSGFLAALAAVLAATLLPWSNFVGHSHWAQVQWIPFYGRRL
                     DWFDIAANVALFVPLGYFAGGFCAVLSPGKKQLLVLAGTTLLSTAAEFIQIYSHGRFP
                     SATDIGCNIVGAALGLMISAGHSSTSAKYRPLATSKSCPRTTGEPIDRQEVIR"
     gene            complement(594..1334)
                     /locus_tag="VE170_05560"
     CDS             complement(594..1334)
                     /locus_tag="VE170_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ14936.1"
                     /translation="MASSGNKILLGKVALITGGSRGIGRALAAAYADQGAQVFICGRN
                     PADIDRTVAEIQKTGGEIAGVPGDIGNAADAARIVAAALGQFSAIDILVNNAGILGPR
                     VAIAEYPIAEWEEVMRINLNGLFFVTHKVLQVMLARQSGSIINVTSGVGRLGKARWGA
                     YAVSKAGLEGFTQVLADEVKDSGIRVNSVNPAATRTAMRAAAYPQEDPSTLPTAESVL
                     PIFIYLASDKSRRVTGQALNARDWRENS"
     gene            1367..2308
                     /locus_tag="VE170_05565"
     CDS             1367..2308
                     /locus_tag="VE170_05565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYJ14937.1"
                     /translation="MTSWQPHQRLLIYVLLALALACAISPVLSLGADWFMTQWPALMP
                     ERIPFHRTFDRAFMIAGILLFIVGRRALIPARLRKLLRVGAATARRDLAVGLCLALGS
                     MLLVVAAMTVTEVFTPFFRLTRSVAIGRVASAAAAGIFAGVFEEIFFRGILFMGLRAH
                     SYKVRAYLLTNLFYSALHFVRPGEAYFLDRLDLGAGFRHLAYTFTPFLDPLPLLPGLF
                     GLLLVGAVLSFAVERAGNLYVAIGLHAGWVFSLKALRVFGDFQRERLGWLFGSTDPKI
                     VSGAVTWAVILLVGVAVYYLTKSRAVRLSDLPRAVTA"
     gene            complement(2256..2909)
                     /locus_tag="VE170_05570"
     CDS             complement(2256..2909)
                     /locus_tag="VE170_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYJ14938.1"
                     /translation="MTKRSMRRAAGYVCVAIALLGCGGDSFDSVRNLRSTNEVIVCLG
                     DSLTEGVGANGGEDYPAVLSRELAFPVLNRGRRGDTTALALGRLPEVLEGNPRLVIVL
                     LGGNDFLRQVPRAETKKNLIELVRRIQAQGAMVAIAGMRVGIFTDEYGPIYEETAKQL
                     GALYIPQVMQGILTDAKLKSDPIHPNGAGYRLMGQRIAEKIRPLLREADRLTGRRGF"
     gene            complement(2906..4279)
                     /locus_tag="VE170_05575"
     CDS             complement(2906..4279)
                     /locus_tag="VE170_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ14939.1"
                     /translation="MESYSERIGKFAGELQLADVPPAVVEKAKLVFLDTLGVALASST
                     MDFGAMVTKVATKLGGAGDSVIIGAPSRVAAANAVIANGTLAHGLDYDDTLEEAIVHT
                     GACAWMTALAVAEETRATGAALLAAAIVGTEVLCKIGLVAPGKFHARGFHPTAICSTF
                     GAAAAAGRLYGLTLPQWIDAFGLCGSQSSGIIEYLADGTWTKRLHPGWSAHGGVIAAL
                     LAQQGFRGPAKVFEGTNGFFPSFGGKNEYHYEKITELGKVWEIPKLTFKSYPCGSISH
                     PYMDCALKIKQRYAPAPARIKEVVCRTAEGPVHRLWEPLADKQKPISSYGAKFSLPYS
                     IAVMLVHGRASLEEFTDLAIGDPLVLSLAAKVRYELDPTIDYPRHFSGHVKVFLDDGT
                     LLEENQPFPRGGFEDPLPPSDIEAKFRANAKLALPLDRIERILDAVQHLEKLALTREL
                     SELLLKP"
     gene            complement(4693..5163)
                     /locus_tag="VE170_05580"
     CDS             complement(4693..5163)
                     /locus_tag="VE170_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ14940.1"
                     /translation="MSKISKILAPTDYSDLSCIGLRRAFETAHESGAELIVLHVIDMS
                     NDWFGKASNLGPVRTLMAEQTEFLDKFLRDKFGALINLVEVRQKIEVGAAHVNIAELA
                     ARESVDLVIMSTHGRTGLDHILLGSVTEKVIAHASCPVLAIPAPKRGESLASAA"
     gene            complement(5202..6113)
                     /locus_tag="VE170_05585"
     CDS             complement(5202..6113)
                     /locus_tag="VE170_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ14941.1"
                     /translation="MAMYAKILIPLDGSTTAEKVLPFARILAGTLKLPVELLEIVDIA
                     AATAHMVADKARYLDTIITEAESARREYLAEIAETFAGVPVTCKVARGRPADVIIESA
                     AADKGTLIAMATHGRSGINRWLMGSVAEKVLRGSTAPLFLVRAARDGASQDRGASIKS
                     MIVPLDGSELAESVLPTVCELAIKLDLEVVLFRAYELAAAAYYGNEAYLPNYDEMLRE
                     LKMEVEGHLQKTAEGLTASGLTKVSWVASPGSDAEEIVRFANQHQDALVAMCTHGRSG
                     VARWTLGSVTEKVVRHAEEPVLVVHAP"
BASE COUNT         1231 a         1930 c         1737 g         1336 t
ORIGIN      
        1 attgtaggat cgacaggaca acaaaaaaat aagtcttcac gggcgggcca ctcgctttca
       61 accccaaccg ccgctatcca tcacatcatg catcggttca ggttatttct tcgcggacgg
      121 ccaaatctaa cggataactt cctgacggtc aattggctca ccggtcgttc tcgggcagga
      181 cttgctggtc gcgaggggcc ggtatttcgc actcgttgat gagtgaccgg ctgaaatcat
      241 cagccccagc gccgcgccca cgatgttgca accgatatcg gtggcggacg gaaaccggcc
      301 gtgcgagtaa atttgtataa actcggccgc tgtcgacagc aaggtcgtcc cggccagcac
      361 caagagctgc ttttttccgg gtgaaagaac ggcgcaaaat ccaccggcga aatagcccaa
      421 tgggacaaaa agcgcgacat tggcggcgat atcgaaccag tccaaccggc ggccgtagaa
      481 tggtatccac tgaacctgag cccaatggga atggccgacg aaattggacc aaggcagcag
      541 agtcgccgcc agcactgcgg cgagcgccgc gaggaagccg ctccagcagc ccatcacgaa
      601 ttttctcgcc agtcgcgcgc gttcaaagcc tggccggtaa cccgtcgcga tttatccgat
      661 gccaggtaga tgaaaatagg caaaaccgat tccgccgtcg gcaaggtcga gggatcttct
      721 tgcggatatg ccgccgctct catggcggtg cgcgttgccg ccgggttgac cgagttcacc
      781 ctgatgccgc tatcttttac ttcatccgcg agcacctgag taaatccctc caagccggct
      841 ttcgagaccg cgtaggcgcc ccaacgcgcc ttgccgagcc ggccgacgcc ggaagtaacg
      901 ttaatgatcg agccgctctg ccgcgccaac atcacctgca ggactttatg ggtaacaaaa
      961 aatagcccgt tgagattgat gcgcatgact tcttcccact cggcgatcgg atactcggcg
     1021 atcgccaccc gcggcccgag gataccggcg ttattgacca aaatatcgat ggcggaaaat
     1081 tgccccagtg cggcggcgac gatgcgcgcc gcgtcggcgg cgttgccgat atctcccggc
     1141 acacccgcga tctcaccgcc ggtcttttga atctctgcca ccgtgcggtc gatgtcggcc
     1201 ggattgcgtc cgcagataaa cacttgggcg ccttgatcgg cataggccgc ggcgagagca
     1261 cggcctatgc cgcggctgcc gccggtaatc agcgcgacct tgccgaggag tattttattg
     1321 ccgctacttg ccatcgtttc gtcgctataa catagttaat tagttgatga catcttggca
     1381 accgcaccag cgactactca tctatgtgtt gctcgcactc gccctggctt gcgccatcag
     1441 cccggtcttg tccctgggcg ccgattggtt catgacccag tggccggcgc tgatgccgga
     1501 acgcatcccg ttccatcgca ccttcgatcg cgccttcatg attgccggca tcctgctgtt
     1561 catcgttggc cggcgcgcgc tgattcccgc gcggttacga aagctgttgc gggtcggcgc
     1621 cgccacggcg cgacgagatc tcgccgtcgg cctgtgcttg gcgctgggct caatgctctt
     1681 ggtcgtcgcc gcgatgaccg tgaccgaagt ttttacgcct ttctttcggc tcacaagatc
     1741 ggtcgccatt ggacgggttg ccagcgcggc ggcggcagga atatttgccg gcgtattcga
     1801 ggaaatattt tttcgcggca tcctgttcat ggggctgcgc gcccatagct acaaggtccg
     1861 tgcttatctc ctgaccaact tgttttattc cgcgctccat ttcgtcagac cgggcgaggc
     1921 ctactttctc gaccgtctcg acctcggcgc cggcttccgt catttggctt ataccttcac
     1981 gccttttctc gacccgttgc cactgctacc cggcctgttt ggcctgctgc tcgtcggcgc
     2041 cgtgctttca tttgccgtgg agcgcgccgg caatttgtat gtcgccatcg gcttgcatgc
     2101 cggatgggtg tttagcttaa aagctttacg agtgttcggc gatttccaac gggagcgatt
     2161 aggctggctc ttcggttcca ccgatccgaa aatcgtcagc ggcgcggtca cctgggcagt
     2221 gatcctgctg gtcggcgtgg cggtatatta cttgactaaa agccgcgccg tccggttaag
     2281 cgatctgcct cgcgcagtaa cggcctgatc ttctcggcga tgcgctgccc catcaaccga
     2341 taacccgcgc cgttgggatg gatcggatcg ctcttgagct tggcgtccgt aagaattcct
     2401 tgcatcacct ggggaatgta aagggcgccg agctgcttgg cggtctcttc gtagattggg
     2461 ccgtattcat cggtaaatat gccgacccgc atgccggcga tcgccaccat ggcgccttga
     2521 gcctgaatgc gccgaaccag ctctattaaa tttttcttgg tttccgcgcg cggcacctgc
     2581 cgcaagaaat cgttgccgcc caataaaaca atcaccagcc gcggattgcc ctcgaggact
     2641 tccggtaacc gccccagcgc cagcgccgtc gtgtcgccgc gccggccgcg gttgagcacc
     2701 gggaaggcca gttcgcgcga cagcaccgcg ggatagtctt cgccgccatt ggcgccgacg
     2761 ccttcggtca agctgtcgcc caaacagact attacctcgt tggtcgatcg caggttgcgc
     2821 acactgtcaa agctgtcgcc gccacaaccc agtagcgcga tcgccacgca aacgtaaccg
     2881 gcggcgcgcc gcatgcttcg cttggtcacg gtttgagcaa caattcgctc agctctcggg
     2941 tcagagctaa tttttccaaa tgctgcaccg cgtcgagaat tctttcgatc cgatccagcg
     3001 gtaacgccag cttggcgttg gcgcggaact tcgcttcgat gtcggacggc ggcagcggat
     3061 cttcaaaacc gccgcgcgga aacggttggt tttcttcgag caaggtgccg tcgtcgagaa
     3121 atactttgac gtggccggag aagtggcgcg gataatcgat ggtcggatcg agctcatagc
     3181 gaactttggc cgcaagactc aggactagag gatcgccaat cgctaggtcg gtgaactcct
     3241 ccaagctggc gcgcccgtgc actaacatca cggcgatgct gtagggcaga ctgaacttgg
     3301 cgccgtaaga gctgatcggt ttctgtttgt ccgccagcgg ctcccacagc cgatgcaccg
     3361 gcccttccgc ggtgcggcac acgacttcct ttatccgcgc tggcgccggc gcataccgct
     3421 gtttaatttt aagggcgcaa tccatgtagg gatgagagat cgaaccgcag ggataggatt
     3481 taaacgtcag cttgggaatc tcccagactt tgccgagttc ggtgattttt tcatagtggt
     3541 actcgttctt gccgccgaac gacggaaaga aaccgttggt gccttcgaaa actttcgccg
     3601 gcccgcgaaa gccctgttgg gccaagagcg cagcaatgac accgccatgg gcagaccagc
     3661 cgggatgcaa tcgcttggtc caggtaccgt ccgccaagta ttcgatgatc cccgagctct
     3721 gactgccgca cagaccgaag gcgtcgatcc attgcggcag ggtcaatcca tagagccgtc
     3781 ccgctgcggc ggcagcgccg aaggtcgagc aaattgccgt cggatgaaaa ccgcgggcgt
     3841 gaaattttcc cggcgccacc aggccgattt tacacagcac ctcggtgccg acgatcgccg
     3901 cggccagcaa cgccgcgccg gtggcccggg tttcttcggc gacggccaat gcggtcatcc
     3961 aggcgcacgc gccggtgtgc acgatcgcct cctccaaggt gtcgtcgtaa tccaagccat
     4021 gcgccaaagt gccgttggcg atcaccgcat tggctgccgc cactcggctt ggcgcaccga
     4081 taatcacgct atcgccggca ccgccgagtt tcgtcgccac tttggtgacc atcgcgccga
     4141 aatccatggt cgacgatgcc agagcgacgc cgagagtatc gagaaacacc agcttggctt
     4201 tttccaccac cgccggcggc acatcggcaa gttgaagctc gccggcaaat ttaccgatcc
     4261 gttcagaata agactccacg ggtggtatct ccttaggact ttttactgaa cccagtttag
     4321 cataaaaccg ctccctccgg ctttcgaccg gcagaaataa gccggagaga tcgcgtcttt
     4381 ataagctcga tgaaacaggc aggtctaagt tagcgaggta ttttaagcta cggcacgcaa
     4441 cgcagagccc gacggcgccg ccggtcgtcg ggcccgttcc aagagccgtt ttttattcgg
     4501 cttgtaatgc gtgaacgatg agcaattaga tgagcgtctg cctcggagga taaagaacaa
     4561 cagagagttt cctaccgcat cgcgcgtcta ctgcagtcgc ttcgatcaac gccggcagtc
     4621 cgcatctcgt ctccgtccga acctttgaac tttgaacact tgaccccttg aacgacatct
     4681 gatcgatatc cgtcaagccg cgctggccaa cgactcaccc cgcttcggcg cgggaatcgc
     4741 cagcaccggg cagctcgcgt gggctatgac tttctcggtg acgctgccga gcagaatatg
     4801 atccagtcca gtgcgcccat gagtcgacat gatcaccaga tccacgcttt cacgggcagc
     4861 gagttctgcg atatttacat gggcggcgcc cacttcgatc ttctgccgga cctccaccag
     4921 attgatcaat gccccgaact tgtcccgtag aaatttatct aagaactccg tctgttcggc
     4981 catcaaggtg cgcaccggtc cgagattact ggctttgccg aaccaatcgt tgctcatgtc
     5041 gatcacgtgc aagacgatca gctccgcgcc ggattcatgg gcggtttcaa aagcgcgccg
     5101 caaaccgata caggacaaat cggaataatc ggtcggcgcc aatattttgc ttatcttgct
     5161 catagtctcc tcctgcaaat cgctgggtta tagattccgg atcagggcgc gtgcactacc
     5221 agcaccggct cttcggcatg acgcacgact ttctcggtga cgctgcccag cgtccaccgc
     5281 gccactccgg atctgccatg ggtacacatc gccaccaacg catcttgatg ctgattggcg
     5341 aagcgcacga tctcctcggc gtccgatcct ggcgatgcca cccacgagac tttggttagc
     5401 cccgatgcgg tcaacccctc ggcggttttc tggagatggc cttccacctc cattttcagc
     5461 tcgcgcagca tctcgtcata gttgggcaga taggcttcgt tgccgtagta cgccgccgcc
     5521 gcgagttcat aggcgcgaaa caggacgacc tcgaggtcga gctttattgc gagttcgcaa
     5581 acggtcggca gcaccgattc cgccagctcc gaaccgtcga gcggcacgat catcgacttg
     5641 atgctggcac cgcggtcctg gctggcgccg tccctggcgg cgcggacgag gaacagcggc
     5701 gctgtgctac cgcgcagcac cttttccgcc acgctgccca tcagccaacg attgattcct
     5761 gagcggccat gggtcgccat ggcgatcagt gtgcctttgt ccgctgcagc gctttcaatg
     5821 atcacgtcag ccggtcggcc tcgcgccacc ttgcaagtga ccggcacacc ggcaaaggtt
     5881 tcagcgatct cggccaaata ctcgcgcctc gcgctttcgg cctcagttat aatcgtgtcc
     5941 agatagcgcg ccttgtcagc gaccatatgc gctgtcgcgg cggcgatatc gacgatttcg
     6001 agcaattcga ccggcagctt caatgtcccg gccaggatac gggcaaatgg caataccttt
     6061 tcggcagtcg tcgaaccatc caacgggatc aatatttttg catacatagc catctcgttt
     6121 ccgggacatc tttcaactgt ataaaaccgc aagcagcatg ccagcggcct tggttgatta
     6181 ttcaatacgc tagactaagg gattgtcagc gttgggaaaa agaccgctca gtta
//
LOCUS       DASQKS010000214         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3473283, whole genome shotgun sequence.
ACCESSION   DASQKS010000214 DASQKS010000000
VERSION     DASQKS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3473283"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VE170_05590"
     CDS             <1..1404
                     /locus_tag="VE170_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ14942.1"
                     /translation="STDLPATLVQQIARNVLSVDASAIPLEVVEHTKALILDSVGCAL
                     AAIEESAFARASRVLAQLGGNPDCTVIGSSRRVNLPQAVLLNGILIRSLDLNDAYIGP
                     GQMGHPSDNIAVALSVGEKFGASGLDVIASVALGYEIYCRMQDIIPARGTWDHTTASA
                     LAAPLIAGRLMGLNAEQLAHAAALSATHGNTLAVVRTGQLANSKANANAMVASQAVLC
                     SLFAAEGVTGSLGVIEHPRGLSTGMLAGANLALLAEPIGERYRILTSAIKAYPCIGTA
                     QTMVAGVLQARAGIADPAREVKRIEVVMADIPIVSTQAADQQRRYPESRESADHSFFY
                     LAAAALSDGEISEAQFAPGRWLQPAMREAMARVSVRTDPSLNTHTPGSYPAVVKLILQ
                     SGESREVEVIFPKGHPNNPMSPAEVETKFRACARGCLTSARQDKVIANARELEKLPSV
                     ADLMQMLAECESGTNQT"
     gene            1427..1876
                     /locus_tag="VE170_05595"
     CDS             1427..1876
                     /locus_tag="VE170_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14943.1"
                     /translation="MSSAKLLTKLKLYSMEVFMPVKRIDVSEHPSVKGIVGRGKHDNL
                     LFNSATSHVWIHNSPPGDKGPMHRHTADQTFYGLEGECWLNFPDGTRQSITPGVLVVI
                     PKGQYYQQEAPSHTGYVFLGTRAETYENSRFGPEGQEIKAGGKAPMD"
     gene            2131..3201
                     /locus_tag="VE170_05600"
     CDS             2131..3201
                     /locus_tag="VE170_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14944.1"
                     /translation="MRVAPRLYHFALAVVTTAVGLLAIKPFSAFAQTPFYQGKAVKVV
                     VAGTPGGTADFRVRALVPFLRKYIPGNPTVLLEFMDGSGGRKAANYMYGNARPDGLTL
                     GALSGGLVALQIMGESGVMYDVDKFIYLGSSEGSSHQIIYTRKELGLDSIEKLRAASG
                     IRVGSQSVGHVAYVSGRLFAQLIGMKDAKFIAGYTAPEVDAALLRGELDARSNNPTSV
                     LRRNPEWLEKGVMNFHSILEAPKGLKHPRFGHLPEIESFAKSEKEIKLLKLWRSFRVI
                     GSPYVLPPGTPQARVKTLKQAVVQAFQDRDFHSEYEKLTGESADLIMPDAMAKIIQET
                     PRDIEVIDTLKKLSGAGPLPPR"
     gene            complement(3552..>4420)
                     /locus_tag="VE170_05605"
     CDS             complement(3552..>4420)
                     /locus_tag="VE170_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYJ14945.1"
                     /translation="LRETEREGWSKSTKFGSRTARLIPAVVDRLEWAGGANAADFAFL
                     RSVTSQTPKITLPGPGYIHYRAGRANISRSVYPDLDRFWSDLVDCYVKELRSLADVGC
                     THLQMDETSFVKLGDARVRAILRDRGDDWSDLLDQYIEAMNAIAEQAPPGMKLAVHIC
                     RSQNPNWQANASYDPIAEKIFNALKFNVYFLEYDNANAGSFEPLRLVPPGKRVVLGLI
                     STRVPEIEDIDGLKRRVEEASRYIPLAQLAIGPQCGFSTNLIEGVHLTNEIQRAKLAL
                     IAAAAQGIWPGG"
BASE COUNT          920 a         1250 c         1311 g          939 t
ORIGIN      
        1 agcactgatt taccagctac cctggtgcaa caaatcgccc gcaacgtgct cagcgttgat
       61 gcttcggcaa ttccactgga ggtcgtcgag cataccaagg cgctgatcct cgactccgtc
      121 ggctgcgctt tggccgcgat cgaggagtcc gcatttgcac gcgcgtcgcg cgttcttgcc
      181 caactcggcg gcaacccgga ctgcaccgtg atcggttcat cgcggcgcgt taatctgccg
      241 caggccgtgt tgctgaatgg aattttgatt cgctcgctgg acttgaacga cgcctacatc
      301 ggcccgggac aaatgggaca tccgagcgac aatatcgcgg tggcgctttc cgtcggtgaa
      361 aagtttggcg cctcgggact ggacgtcatc gccagcgtag cgctcggcta cgagatttac
      421 tgccgcatgc aggacatcat tccagctcgc ggcacctggg atcacacgac tgcgtcggca
      481 ttggcggcgc ctttgatcgc cggccggctg atgggattga acgcggagca gcttgcccat
      541 gcggcggcgc tgtcggccac ccatggcaat accctcgccg tcgtgcgcac cgggcagctg
      601 gccaattcca aagccaacgc caacgccatg gtggcatccc aagcggtatt gtgttcgttg
      661 tttgccgccg agggcgtgac cggatcacta ggagtgatcg aacatccgcg cgggctgagc
      721 actggtatgc tcgccggggc aaatttggct cttctcgctg aaccgatcgg cgaacgctat
      781 cgaatcctga cctcggctat caaagcctat ccctgtatcg gcacggccca gaccatggtc
      841 gccggcgtgc tgcaagcgcg cgccggcatc gccgacccgg cgcgcgaagt taagcgcatt
      901 gaggtggtga tggccgatat tccgatcgtc agcactcaag cggcggacca acaacggcgc
      961 tacccggaaa gccgcgaaag cgcggatcat agcttcttct atcttgcggc agcggcgctt
     1021 tcggacggcg aaatttctga agcgcaattt gcccccgggc gctggctgca acccgcaatg
     1081 agagaagcga tggcgcgagt ctcggtgcgc accgatccga gtttgaacac gcacacgccg
     1141 ggcagctacc cggcggtggt gaaactgatt ttgcaaagcg gcgagagccg ggaagtcgag
     1201 gtgatttttc ccaagggcca tcccaacaat cccatgtcgc cggccgaagt cgagaccaaa
     1261 ttccgcgcct gcgcgcgggg atgcctgact tcggcgcgcc aggataaagt tatcgcgaat
     1321 gcgcgcgagt tggaaaagct cccgtccgtg gccgatttga tgcaaatgtt ggcggaatgc
     1381 gaaagcggta cgaaccagac ttgaactgcg caatagggat agcaacgtga gcagtgcgaa
     1441 gctgttaaca aaactgaaat tatattcaat ggaggtcttt atgccggtta agcgtatcga
     1501 tgttagcgag cacccgtctg taaaaggaat tgtcggtcgt ggcaagcatg acaatctact
     1561 ttttaactcc gctacctctc atgtctggat tcacaatagc cctccgggtg acaaaggtcc
     1621 gatgcacaga cataccgcgg atcaaacctt ctacggtcta gagggcgaat gctggctaaa
     1681 tttccctgat ggaacccggc aatcaattac ccctggtgtc ttggtcgtga ttcccaaagg
     1741 acaatattac cagcaggaag cgccgagcca cacgggttat gtattcttgg gaacgagggc
     1801 agaaacctat gaaaactccc gctttggccc cgaagggcaa gagatcaagg cgggtggtaa
     1861 agcaccgatg gattagcttc ggatgtgatt agcctgcgat gccggctcaa gcaatcgtac
     1921 tgccttcaaa tcactgagca ctcgcggttt cggcggcggt ttgttcgtac gctaactcgc
     1981 gctcagcgat cactgcggct atcattccta tggttgattt tgttactttt cggagaagcg
     2041 cggctagccc ggcagaggtg aatgatgatg ccaactagat ggtttggaag ttttcgtatt
     2101 tcactgtcgc ctcaaccggc acttatgtcg atgcgcgttg cgcccagact ctatcacttc
     2161 gcattggcgg ttgtgacgac ggctgttgga ctactggcga tcaagccgtt ttctgcgttc
     2221 gcgcagactc cgttctatca gggaaaagcg gtgaaagtgg tcgtcgctgg cacaccgggt
     2281 ggcacggctg atttccgcgt tcgagcgctg gttcccttcc tgcgcaaata catcccaggc
     2341 aaccccacgg tccttttgga gttcatggac ggtagcggtg gacgaaaagc ggccaattac
     2401 atgtacggca atgcccgccc agacggtctc actctaggcg cgctcagtgg cggtctcgtc
     2461 gcgctgcaaa ttatgggcga gtccggcgtc atgtacgacg tcgataaatt tatctatctc
     2521 ggctcctcgg aaggcagtag ccatcaaatc atttatacga ggaaagaact cggcctcgac
     2581 agtatcgaaa agctgcgggc ggcctcgggc atccgggttg gttcccaatc ggtcggccac
     2641 gtcgcctatg tatcgggacg gctatttgcg cagttgatcg gcatgaaaga tgccaaattt
     2701 atcgctggct atacagctcc agaggtggac gcggcgctgc tgcgcggcga attggacgcg
     2761 cgctcgaaca acccgacctc ggtgctcagg cgtaaccccg agtggttgga aaaaggcgtg
     2821 atgaatttcc actcgatcct cgaagcgccg aaggggctca aacacccgcg ctttggccac
     2881 ttgcctgaga tcgaaagctt cgccaaatcg gaaaaagaaa ttaagctcct caagttgtgg
     2941 cgctcattca gagtgatcgg ttcgccgtat gttttgccgc cgggcacgcc ccaggctcga
     3001 gtgaaaactc tcaaacaggc ggtcgttcaa gcttttcagg atcgcgactt ccattccgaa
     3061 tacgagaagc ttaccgggga aagcgcggac ttgatcatgc cggacgcgat ggcaaagatt
     3121 atccaggaga cgccgcgcga tattgaagtg atcgacacac tcaagaagct gtccggcgcc
     3181 gggccgctgc cgccgcgtta gcgcggcccg gtgaaagccg agtcaatggt atcgggctgg
     3241 caagcgtcgc gcggatttct aacttacttt taacatcgag gtgaggtcag agattcaacc
     3301 gatgtggggc gatcccggct aacttggttg cagcagagga cgaaaaagac tcccggtacc
     3361 tttttctgtc cgtccccgac atcttcaacg gttcgagatt ggtcgtacgc tcgcgccacc
     3421 atcagatagc gaggcgagca gttgcgcatg ccagatgcct agcggatcga tctgcagctg
     3481 atcttcttca ttccgagtgt ggcccgcatt ttctttgcgc cgcgtcgatc cattctttgt
     3541 tctcatcgtt ctcatccccc gggccagatt ccctgcgcgg cggctgcaat tagcgccaat
     3601 ttcgcgcgct gaatttcatt ggtgagatgc acgccctcga ttaggttagt cgagaagccg
     3661 cactgtggac cgatcgccaa ttgcgccagc ggaatatagc ggctcgcctc ctcgacgcga
     3721 cgtttcaggc catcgatatc ctcgatctcg ggaacacgcg tggatatcag gccgagtacg
     3781 acgcgcttgc ccgggggcac gaggcgcagg ggctcgaagg agccggcatt ggcgttgtcg
     3841 tactccagaa aatatacgtt gaacttgagc gcattgaaaa tcttctcggc aatcgggtcg
     3901 tagctggcgt tggcctgcca attggggttc tggctgcggc agatgtgcac ggcgagcttc
     3961 atgcccggcg gcgcctgttc ggcgatggcg ttcatcgcct cgatgtattg gtcgagaagg
     4021 tcggaccaat cgtcgccgcg atcgcgcagg atggcgcgga ccctcgcgtc gcccaatttg
     4081 acgaaggacg tctcgtccat ctgcaggtgc gtgcaaccga catccgcgag cgagcgcaat
     4141 tccttcacgt agcagtcgac aagatcggac cagaagcggt caagatcggg ataaacgctt
     4201 cgactgatgt tggcgcgacc cgcgcgatag tggatgtagc caggtccagg caaggtgatt
     4261 tttggggttt ggctggtgac ggatcgaaga aaggcgaaat ccgcggcgtt ggcaccgccg
     4321 gcccattcga ggcgatcgac gacggccgga atgagccgag cggtccgcga cccgaacttt
     4381 gtcgatttag accacccctc acgctcggtt tcccgcagtt
//
LOCUS       DASQKS010000215         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3891035, whole genome shotgun sequence.
ACCESSION   DASQKS010000215 DASQKS010000000
VERSION     DASQKS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3891035"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VE170_05610"
     CDS             <1..475
                     /locus_tag="VE170_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYJ14946.1"
                     /translation="DKSSDPESAKKAYEHMGWTPGAPIIGTPVNVAFIGSCTNGRLAD
                     FRAAAKIAKGRKVNPSVRALVVPGSAEVKKLAEAEGLDKIFEEAGFQWREAGCSMCLA
                     MNPDKLNGREVSASSSNRNFIGRQGSPQGRTLLMSPAMVVAAALNGKVVDVRQYE"
     gene            607..1221
                     /gene="leuD"
                     /locus_tag="VE170_05615"
     CDS             607..1221
                     /gene="leuD"
                     /locus_tag="VE170_05615"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYJ14947.1"
                     /translation="MSEIIKIVKITGTALPMRGNEIDTDRIIPARYLKEVTFARMGDY
                     PFFDERYSSEGKKKDHPFNDPEYSGASILFVNKNFGCGSSREHAPQALNRFGIKVIVG
                     ESFASIFAGNCTMMGVPTVTASEKEIQQLIKSVEENPKTEYTVDLDNKTLTYGKVKIA
                     IDLPETYRTALTMGSWDSTALLRANLEQVKQTANKLPYMNSFAG"
     gene            1257..1955
                     /locus_tag="VE170_05620"
     CDS             1257..1955
                     /locus_tag="VE170_05620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ14948.1"
                     /translation="MAERIVSMHAAHRSGREVVDGFLSRPTGAGERPAIVLVHGYRGL
                     DDGQRAVTRRFAQEGFVCLSPDLFHGKTYHTLEDCALKKTSLDIPKAVNNIVDAVPYL
                     RKLPWVGKKKVAVLGFCMGGGLALYALAQSKDFAAGVIYYQSMFPDPEDLKSINAPML
                     CHYGTADAGTTQTEIDMFRDSLNRHKKKFQIEMYAGAGHAFLNNPSGKNAANRDAAVK
                     SVACTAKWLRKTLA"
     gene            1982..2287
                     /locus_tag="VE170_05625"
     CDS             1982..2287
                     /locus_tag="VE170_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HYJ14949.1"
                     /translation="MANKWDERKKAQEEEYFVKKEREQLAKLKAKQEAEASETAKKAA
                     HMRCPKCGEPLKARSFQKIEIDQCTSCSGIWLDAGELEQVAEKDEGSWLGKFWQKNS"
     gene            2409..>2822
                     /locus_tag="VE170_05630"
     CDS             2409..>2822
                     /locus_tag="VE170_05630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ14950.1"
                     /translation="MKKIASSLLLALAVMWLQTTALGLAQEGKIVPYVPTPQEVVDRM
                     LELAQVKKGDVVYDLGSGDGRIVVTAAKKYGVKAIGFEIDPERIKESHENIKKAGVEK
                     LVEIRQQDIRTVDLSAANVLTMYLLPEVNLMIRPNI"
BASE COUNT          708 a          738 c          843 g          533 t
ORIGIN      
        1 ggacaagtcg agcgatcccg agagcgcgaa gaaagcctac gagcatatgg gctggacgcc
       61 gggtgcgccg atcatcggca cgccggtcaa cgttgcgttt atcggttcct gcaccaacgg
      121 ccggcttgcg gattttcgcg cggcggcaaa gattgccaag gggcgcaagg taaatcctag
      181 cgtgcgtgct ctcgtcgtcc cgggttctgc tgaagtcaaa aagctcgccg aggccgaggg
      241 gctggataaa atattcgaag aagcaggatt tcaatggcgc gaagccggct gttcgatgtg
      301 cctggcgatg aatcccgaca agctcaacgg cagggaggtc tccgcgtcgt cgtccaaccg
      361 caatttcatc ggccgccagg gcagcccgca gggccgcact ctattgatga gtcctgcgat
      421 ggtggtggcg gcggcgctca acggcaaagt cgtcgacgtg cgccagtacg aatgagagaa
      481 cgacgttcaa atcgttcaaa aaagttcaag ccgtttaaat cgttccgcgt ctataaacgg
      541 cttgaacgga gcgcggcggt tgaacggttt gaacgacttg aactgatttg gacctactga
      601 ggaaccatga gcgaaataat aaagatcgtc aaaatcaccg gcacggcgct gcccatgcgc
      661 ggcaacgaaa tcgacaccga ccggatcatt ccggcgcgtt atctaaaaga agtgacgttc
      721 gcgcgcatgg gcgactatcc gttcttcgat gagcgctaca gcagcgaagg caagaagaaa
      781 gatcatccct tcaacgaccc cgaatatagc ggcgcgtcga tcttgttcgt caacaagaat
      841 ttcggctgcg gttcgtcgcg cgagcacgcg ccgcaagcgc tcaaccgctt cggtatcaaa
      901 gtgatcgtcg gtgaatcctt cgcgtcgatc ttcgccggca actgcaccat gatgggcgtg
      961 ccgacagtga cggcgagtga gaaagaaatt cagcagttga ttaaaagcgt cgaggagaat
     1021 cccaagaccg aatataccgt cgacctcgac aacaaaaccc tgacctacgg caaagtgaag
     1081 atcgccatcg atctgccgga aacctaccgc acggcattga ccatgggatc ctgggactcc
     1141 acggcgctct tgcgcgccaa cctcgaacag gtcaaacaga ccgccaacaa actgccctac
     1201 atgaatagct tcgccggcta acgccggcga gcgcaatact gggagcaagc cgaggaatgg
     1261 ctgaaaggat cgtatccatg catgccgcgc atcgcagcgg cagggaagtc gtcgatggtt
     1321 ttttgtcccg gcccaccggc gcgggcgagc gcccggcgat cgttttggtg catggttatc
     1381 gcggcctgga cgacggccag cgagcggtga cgcggcgctt tgcccaggag ggatttgtct
     1441 gtctatcgcc ggatctgttt catggcaaga cctaccacac gctcgaagat tgcgcgctga
     1501 aaaagacctc gttggatatt cccaaagccg tcaacaatat cgtcgacgcc gtgccttatt
     1561 tgcgcaaact gccctgggtc ggcaagaaga aagtcgcggt attgggcttc tgcatgggcg
     1621 gcggtttagc gctatacgct ttggcgcagt cgaaggactt cgccgccggg gtgatttatt
     1681 accagagcat gttccccgac ccagaagact taaagagcat caatgcgccg atgctttgcc
     1741 attacggcac cgccgatgcc ggcacgacgc agaccgagat cgacatgttc cgtgacagct
     1801 tgaaccgcca taaaaagaag tttcaaatcg agatgtatgc gggggccggt cacgcctttc
     1861 tcaacaatcc gtcggggaag aacgcggcta accgcgatgc cgccgttaag tcggtggcct
     1921 gcaccgcgaa gtggctcaga aaaactctag cgtaactctg agaatatcca aggaggatga
     1981 gatggccaac aagtgggacg agcgcaagaa agcccaggaa gaagagtact ttgtcaaaaa
     2041 agaacgagaa cagctggcga agttgaaagc caagcaggaa gcggaagcca gcgagacggc
     2101 gaaaaaagcc gcgcatatgc gctgtccgaa atgcggtgag ccgctcaaag cgcgcagctt
     2161 tcagaagatc gagatcgatc agtgcaccag ctgcagcggt atttggttgg atgccgggga
     2221 gctggagcag gtggccgaga aggatgaagg cagctggctc ggaaagttct ggcagaaaaa
     2281 cagttagccg tggttgtcga cgcggcgcaa gcgaaacgtt ggccgcaaag aacgcagagt
     2341 acccgtcgag aagtgctgca taaaaccgat ctcgaaatca taactttggt taattcaagg
     2401 aggcgaccgt gaaaaaaatc gcaagttctc tgttgctcgc attggcggtg atgtggttac
     2461 aaaccacggc cttaggtttg gcgcaggaag gcaagatcgt tccctatgtg ccgacgcctc
     2521 aggaagtcgt cgaccggatg cttgagttgg ctcaagttaa gaagggcgat gtcgtctacg
     2581 atctcggatc gggcgacgga cgcattgtcg tcacggcggc gaagaaatac ggtgtcaagg
     2641 cgatcggctt tgaaatcgat ccggagcgga tcaaagagtc tcatgagaac atcaagaaag
     2701 ccggggtcga aaaactcgtg gaaatccgcc agcaggatat ccgcaccgtc gatctgtcag
     2761 ccgccaatgt tttgacgatg tatttgttgc ctgaggtaaa cttgatgatc cggccgaata
     2821 tt
//
LOCUS       DASQKS010000216         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_533987, whole genome shotgun sequence.
ACCESSION   DASQKS010000216 DASQKS010000000
VERSION     DASQKS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533987"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1568)
                     /locus_tag="VE170_05635"
     CDS             complement(54..1568)
                     /locus_tag="VE170_05635"
                     /inference="COORDINATES: protein motif:HMM:NF039926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HYJ14951.1"
                     /translation="MADIKLNEQQLDAQLSQLEQARPWSPRVMSKFETFIRSAPDFDL
                     FRVNPIQYAMERSMTEAEGLDIFLHAAKVGLFEMDWHIVCPHCGYVIDSLHNMSQLRT
                     HYLCPICAAEEEFSLDDYIQVAFSISPLVRDIQYHHPESLSIEDLYFNYRLSKDVISP
                     VPALPAWRDVIMHVTKYLRYLEPGEKVTAEFELPPGDLRATGGRACVQLSMTHEQSKQ
                     ASVVPLRLVNGKFQSDDPEMQPRSLTRYTAENQPVQFGFDLERQLPSGRLVIELANMQ
                     DHRSALWLFFPGEMPTPVLALRPGLSGKKLLTTQTFRDLFRSEVINSDETLSIKDITF
                     LFTDLKGSTAMYEQIGDAKAYFLVHQHFDALRRVIRERNGAIVKTIGDAVMAVFGNPV
                     EATSAALEMIDALNEFNRTISQELILKVGIHRGHSMAVTVNDRIDYFGQNVNIAARVQ
                     ALAGANEVYITAEVFNSPGVSEALKAHQVVPDEVLVKGVIEKLQVYKINHHRND"
     gene            complement(1865..2806)
                     /locus_tag="VE170_05640"
     CDS             complement(1865..2806)
                     /locus_tag="VE170_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14952.1"
                     /translation="MIVLGLLLRQPHTYAQELRPILYGTTASPSHLPIWVAKDAGLFE
                     KAGLNVEPVQIRGGSLSTLAIITGNLQFSGAGAESVVAARAAGGDIALLACPVNADPV
                     YLITRPEIKSAQELKGLSSAVTRYGSTTHFYLRAALKHVGLNPEKDMTILQMGAGPEM
                     ALALERGVIAAAALTTRYAMPFLQKGWPVLVDLSNTDLVYPSSCVTSSRAFIRAEPKV
                     TEDFLRAYVAGIHLTKTHHQAAEKSFAKWMREKDPAITKRTVQAYAKLFKTNPAVPDK
                     GIQNVILDLVRARPDFKEYIGWPEPFRENGPLERALK"
     gene            complement(2950..3930)
                     /locus_tag="VE170_05645"
     CDS             complement(2950..3930)
                     /locus_tag="VE170_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14953.1"
                     /translation="MSVGIHGALVLFVLSVGPVLFSESSPAQELRRIHYGTTASTAHL
                     PIWVAKDAGFFAKQGLNVEPVHIRGGALITMGIMSGQLQFSGAGAESIVAARIEGGDV
                     VLLACPVDSDPVYFITRPEIKSAAELKGKATAVTRLGSTTHFYMRAAARHVGLDPEKD
                     MTILQLGTGSETVSALENGRIAAAAITHRYALPFLQKGWPLLVDLSTTDLVYPSSCVA
                     SSRSYVKSEPKLVESFLRAYVAGIQLIRSDYAFAEKSLAKWLRERDPVVVRKTVEAYA
                     KLFKPVPNVPDKGLENVIRDLQGRRNVPKEFVGRYELFRDSGPLERALGK"
     gene            complement(3953..4423)
                     /locus_tag="VE170_05650"
     CDS             complement(3953..4423)
                     /locus_tag="VE170_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ14954.1"
                     /translation="MPLHVKASEMQEYQRDQSGEAGTGQMVVRKGYGNECSLMIATRR
                     PGYHTKPHQHESEQINYVLDGEIWFFVKDHGYLCKKGDFHRIPANTVHWAFNRSDKDA
                     TVAEAHAPGLIGGRAGDNANALFDDGEKPAIRGPGVNQFVPFDQEGAEAKCFGK"
BASE COUNT          959 a         1278 c         1379 g         1072 t
ORIGIN      
        1 tgtttgagaa ggccgttccc taacttcatc gaactccaac ggcccatttg acttcaatcg
       61 ttgcgatgat ggttgatctt atagacctgc agtttttcga tgacgccctt gaccaacacc
      121 tcatccggga caacttgatg agccttgagg gcttcgctga cacccggcga gttgaagact
      181 tcggcggtta tgtacacctc attggcgccg gcaagggctt gtacccgcgc cgcgatattt
      241 acattttgcc cgaaatagtc gatgcggtca ttgaccgtca cggccatgga gtgcccccgg
      301 tggatgccca cctttagaat caactcctgc gagatcgtgc ggttgaattc gttgagcgcg
      361 tcgatcatct caagggcggc gctggtcgcc tcgaccgggt tgccgaacac tgccattact
      421 gcatcaccga tggttttcac gatcgcgcca ttcctctcgc ggatcacgcg gcgcagggca
      481 tcgaagtgct gatgcaccag aaaataggct tttgcgtcgc cgatctgctc atacatggcg
      541 gtcgaacctt tcagatcggt gaacaagaac gtgatgtcct tgatcgacag tgtttcgtcg
      601 ctgttgatga cctccgaccg gaacagatcg cggaaggttt gcgtcgtcag cagcttcttg
      661 ccgctcaaac ccggcctcaa ggcaagcacg ggggtcggca tttccccggg gaaaaaaagc
      721 cacaacgcgc tacggtgatc ctgcatattc gctaattcaa ttaccaacct gccgctgggc
      781 agttgccgct caagatcaaa gccaaattgc accggctgat tttccgcggt gtaacgggtg
      841 aggctgcgcg gctgcatctc tggatcatcc gactggaatt tgccgttgac caatctaagc
      901 gggaccacgc ttgcctgctt actctgctca tgcgtcatcg acagctggac acaagcccgg
      961 ccgccggtgg cccgcagatc tcccggtggt agttcaaact cggccgtcac tttctcgccg
     1021 ggctccaaat agcgtaaata tttggtgacg tgcataatca catcgcgcca cgcgggcaac
     1081 gccggcacgg gagaaatcac gtctttactc aggcggtaat tgaagtacag atcttctatg
     1141 ctcagcgact ccggatgatg gtactggatg tcgcgcacca gcggtgaaat cgaaaaggcg
     1201 acctgaatat aatcgtccag cgaaaactcc tcttcagccg cacagatcgg gcatagataa
     1261 tgggtgcgga gctggctcat gttgtgcaaa ctgtcgatga catagccgca gtgtgggcag
     1321 acgatatgcc agtccatctc gaatagccca actttggccg cgtgcaagaa aatatccaac
     1381 ccctcggctt cggtcatgct gcgctccatt gcatactgga tcggattgac acggaacaaa
     1441 tcaaaatccg gggcagagcg aatgaatgtt tcaaacttgg acatgacgcg cggactccag
     1501 ggacgcgcct gttccaattg cgaaagttgc gcgtcgagtt gctgttcatt caatttgata
     1561 tcggccatgg ccataacgtc ctttcagtcg agatacactg gaaatgatct ctaataaatc
     1621 gatgcgtcat cactgggctc gacaacttgg caggggcaaa gagtcgtcgc ttccatggcg
     1681 ctgaccgctc ttccatcctg gaatttgcat ctaataaaca attgacactg gatatagtga
     1741 ctctgttggc ggcggcgctg ttgtaggtat cgatggctta tgctcggtac acccggctgt
     1801 attgacgaat tgtatggccg ccaaggccaa gcattcatga cggcgtcaaa gctcaagcca
     1861 atttttattt tagtgcccgc tccaacggcc cattttccct gaacggttcc ggccagccga
     1921 tgtactcctt gaagtcgggg cgggctctga ccaggtcgag gatgacgttt tggatgcctt
     1981 tgtcggggac ggcggggttg gttttaaata gcttggcgta ggcctggacg gtgcgtttgg
     2041 tgatcgctgg atctttctcg cgcatccact tggcaaagga tttttcggca gcttggtgat
     2101 gggttttggt caaatgtatt cccgcgacgt aggcgcgtag aaagtcttcc gttactttgg
     2161 gttcggcgcg gataaaggcg cggctgctgg taacgcagga cgatgggtag accaggtcgg
     2221 tgttgctcaa gtcgacgagc accggccagc ccttttgcaa gaacggcatt gcgtagcgcg
     2281 tggtcagcgc ggcggcggct atgacgccgc gctccaatgc cagcgccatt tcggggccgg
     2341 cgcccatttg caagatcgtc atgtcttttt ctggattcag accgacgtgt ttgagggcgg
     2401 cgcgcagata gaagtgcgtc gtcgaaccgt agcgggtgac ggcgctggac aagcctttga
     2461 gttcttgagc cgacttaatc tccggccggg taatcaagta gaccgggtca gcgttaaccg
     2521 ggcaggcgag caaggcgata tcgccgccgg cggcgcgggc ggcgactacc gattcggcac
     2581 cggcaccgga gaactgcaag ttgccggtta tgatcgcgag cgtgctcaac gaaccgccgc
     2641 ggatttgtac cggctcgaca ttcaagccgg ctttttcaaa cagtccggcg tccttggcaa
     2701 cccagatcgg taaatgcgac ggcgatgccg tagtgccgta aaggataggg cggagttctt
     2761 gcgcgtaggt gtgcggttga cgcaacaaca gaccaaggac aatcatgcaa agcgatccga
     2821 cgctgcgaag tgtgtaaagg gtagttgcct tcgaggcgtt acccgcaggg tggttgtccg
     2881 ctggcagacg gcgaaggcat cggcaaacgg cggcgatcgc catgcgaatc ttgcgcctgt
     2941 tagctaacat tatttcccca aggcgcgctc caatgggccg ctgtcgcgaa ataactcgta
     3001 gcggccgaca aactctttcg gcacgttacg ccgtccttgc agatcgcgga tgacgttttc
     3061 cagccctttg tcggggacat tgggcaccgg cttgaataac ttggcgtagg cttcgacggt
     3121 cttgcgcacc accaccggat ctctttcgcg caaccacttg gccaaggatt tttcggcaaa
     3181 ggcgtaatcg ctgcgtatca gttggatgcc ggcgacgtag gcgcgcagga aactttcgac
     3241 gagcttgggt tcggatttca catagcttcg actactcgca acacaagacg acggatagac
     3301 cagatcggtg gtgctcaagt cgacgagcag cggccagcct ttttgcaaga acggcaaggc
     3361 atagcgatgg gtgatcgcag cggcggcgat gcggccgttc tccagcgccg acacggtttc
     3421 gctgccggtg cccagttgca atatcgtcat gtccttttcc gggtcgaggc cgacgtgccg
     3481 ggcggcggcg cgcatgtaga aatgggtcgt cgagcccagg cgggtgaccg cggtggcttt
     3541 gccttttaat tcagcggcgg attttatctc cggccgagtg atgaaataga ccggatcgga
     3601 gtccaccggg caggcgagca gtacaacatc gccgccttcg atgcgcgcgg cgactatcga
     3661 ttccgcgccg gcgccggaga actgcaactg gccgctcatg atgcccatgg taatcagcgc
     3721 accgccgcgg atatgcaccg gctcgacatt caaaccctgc ttggcgaaaa agccggcgtc
     3781 cttggccacc cagatcggca ggtgcgcggt ggatgcggtg gtgccgtagt ggatgcggcg
     3841 cagctcctgg gctggagatg attcggagaa taggaccggt ccgacggata ggacgaacag
     3901 gaccaaagct ccatgaatac cgactgacat gtctcgacct ctttgctcgg tgctacttgc
     3961 caaagcactt cgcttcggcg ccttcctgat cgaaggggac gaactggttg acacctgggc
     4021 cgcggattgc gggtttttcg ccgtcgtcga acaacgcgtt agcgttgtcg ccggcgcggc
     4081 cgccgatgag gccgggcgcg tgagcttcgg ccaccgtcgc gtctttgtcc gagcgattga
     4141 atgcccagtg cacggtgttg gcggggatgc ggtgaaaatc gcctttttta cagagatagc
     4201 cgtggtcttt aacgaagaac cagatctcgc cgtccaagac gtaattgatc tgttccgact
     4261 cgtgctgatg cggtttggta tgatatccag ggcggcgcgt ggcgatcatc aggctgcact
     4321 cgttgccgta acccttgcgc acgaccatct ggccagtgcc ggcctcaccg ctttgatcac
     4381 gttggtattc ctgcatctcg ctagctttga catgtaacgg catggttgcc tccctagaca
     4441 ttcaagatgg tgaattggta cgccaagtcc gggggagcgt caagcgcttt gtgttggtaa
     4501 aactgagagt ttgcgttact cgcgcaaatt ttctcaccga gacttttcgg acactaatat
     4561 tgctcgcgcc aacgccgcca cgttcgaaaa gttgggaatt catttatttc gttcctttgc
     4621 gccatttgcg tgttttgtgc gagatattgc aaattacggt tgcggctatg ccgcgttcgg
     4681 taaatcat
//
LOCUS       DASQKS010000217         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4732333, whole genome shotgun sequence.
ACCESSION   DASQKS010000217 DASQKS010000000
VERSION     DASQKS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732333"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VE170_05655"
     CDS             complement(<1..226)
                     /locus_tag="VE170_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14955.1"
                     /translation="MGYLPKAGVGFELIEFRSNGRDVQGGIFSPDAKLFEPSSTAVVL
                     VHGVESYWYSAPTMFLGAYLAEAGYTVLGYN"
     gene            430..1176
                     /locus_tag="VE170_05660"
     CDS             430..1176
                     /locus_tag="VE170_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ14956.1"
                     /translation="MIAQSVKDQLAPSGKLRIGINYGNAVLAKRDGASGELRGVAVDL
                     ARELGRRCQLAVELVAFESAGKMAAAVKAGAWDAAFLAVEPGRADELAFTAPYLLIEG
                     TYLTPPGSPIRSIADVDRAGVRIGVSTGSAYELFLSRNLRQAQTIKAENPNTTFDLLI
                     DGKVDVVAGVRQALLANAAKLPGSRVFDERFMAIEQAMGMPHGREAGLQILREFIEEM
                     KASGFVAQALERAGIRGVAVAPADAPEQGI"
     gene            1391..2704
                     /locus_tag="VE170_05665"
     CDS             1391..2704
                     /locus_tag="VE170_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ14957.1"
                     /translation="MDENDHQPAPRPPFMPPFYYGWFIIGLSFLAYLAASAVRAAPSV
                     LIHPLEREFGWGRTAISSAASLNLLTYGLMAPVGGWLLDRYGARRVVIGSLLTIAVGV
                     TGTVFVRELWHFVLLWGIVLGIATGVTPALSASVASRWFIARRGLALGILTNANATGQ
                     VIFLPILMALIVSHGWRPAMMLIVGTALLLLPAIWLWLRDYPAELGLEPYSDGTTTSD
                     QRNQALAAPRVFSLASFGDAIKTPTFWLLSGSFFVCGMTSNGLVGTHMIPHAIERGIA
                     EVTAATAVGIMGVASFVGTTFAGWLCDRVDARKVLSAAYLFRGLSLFVLPYVTDSSGL
                     FVFAVLYGFDWFATGPSTVALIAKHFGQDRVGVLFGLVFVSHQIGSAIAALGGGWIYS
                     EFGEYYYAFMSGAVMGLLAAGMAMMIRRDRDQAPAVPVSTAAVRA"
     gene            complement(2767..>3510)
                     /locus_tag="VE170_05670"
     CDS             complement(2767..>3510)
                     /locus_tag="VE170_05670"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14958.1"
                     /translation="TVLGAAAAGAELKMIANFVSRGKGHLLVRSDIKKPADLAGKRIG
                     VQSIGGTLWMYVTLALEQLGLDLTRDKIRLLIIGDQTVTVRALESQVIDATVLTTRTY
                     IPGLLQKGFNVLTEVNPAMAATGMVTRKNYLEKNPALLENVLKALIEGEYFSLAPSGK
                     AQTIKSIMSHLRLTDSASAEEAHTDLAKEFEAKPYPSLEGLKNMQRLLAMQNPKLADV
                     NPASLIDSSFIRKLDDSGFFAQMQARYRD"
BASE COUNT          637 a          953 c         1106 g          814 t
ORIGIN      
        1 cgttgtagcc cagcacggtg tagccggctt cggcgagata ggcgccgaga aacatcgtcg
       61 gcgccgagta ccagtaggat tcgacgccgt gcactaagac cacagcggtc gatgacggct
      121 cgaataattt agcatcgggg ctgaagatgc cgccctgcac gtcgcgaccg ttactgcgaa
      181 attcgatgag ttcaaatccg acgccggctt tcggtaagta gcccatggaa aatttctcct
      241 gtcacttggt attaccgatc ggtgcgctgc gcaagtggtc atgaacagcg cgtgaaaact
      301 gaaaattgac aatggatagt tgacaatggc ggatcgagca tggcgttgtt tccgtgttgc
      361 ttccttgacg cattggtctg cagcgcgtag cgtcacgcat ggtaattaac cttcataagg
      421 aatgaatgaa tgatcgccca atcagttaaa gatcaactcg cgccgtcggg caaactgcgt
      481 atcggcatca attatggcaa cgcggtgctg gcgaagcggg atggcgccag cggcgagcta
      541 cgcggcgtcg cggtggattt ggcccgggag ttgggccggc gctgccagct ggcggtggag
      601 ctcgtggcct ttgaatcggc tgggaaaatg gccgcggcag tgaaagccgg cgcttgggat
      661 gcggcgtttc tcgcggtgga gccggggcgg gccgatgagc tcgctttcac cgcgccttat
      721 ttgttgatcg agggcactta tttgacaccg ccgggatcgc cgattcgatc tatcgccgat
      781 gtcgatcggg cgggcgtacg gatcggagtg tcgacgggca gcgcttacga gttgttcttg
      841 agccgcaatt tgcgccaggc gcaaacgata aaagcggaga acccaaatac gaccttcgac
      901 ctgctcattg acggcaaggt cgacgtggtt gccggtgtgc gccaggcgct gcttgccaat
      961 gccgctaaac tgccgggttc gcgggtgttc gacgagcgct tcatggccat cgaacaggcg
     1021 atgggcatgc cgcatgggcg cgaagccgga ttgcagatac tgcgtgaatt tatcgaagaa
     1081 atgaaagcca gcggctttgt cgcgcaggct cttgagcgcg ccggcattcg cggcgtggcc
     1141 gtggcgcccg ccgacgcacc ggagcaaggg atttagccgc aaagaacgca aagacgcaaa
     1201 gaaattttgg cagagaattg accccagaaa cgccgcggcc acagagttga gatggaaatc
     1261 ttgtcacaat tctttctctg tgtttcgcgc gtcctcgcgg tgcgatccga ttttcttttc
     1321 cggatagttc taacgtaagg atatggctgc ttgcattatg tcgcgcggtc gcttgtgata
     1381 gacgaacctg atggacgaga acgatcacca gccggcgccg cgcccgccgt tcatgccgcc
     1441 gttctattac ggttggttca tcattgggct gagctttctc gcgtatctcg ccgcgtcggc
     1501 ggtgcgcgct gcgccttcgg tattgataca tcctttggaa cgggagtttg gttggggacg
     1561 aacggcgatc tcttcggcgg cgagcttgaa tttactcacc tatggtttga tggcgccggt
     1621 gggcggctgg ctgctcgatc gttacggcgc gcgtcgcgtc gtgatcggct ctcttttgac
     1681 gatcgccgtc ggcgtcaccg gcaccgtgtt cgtccgtgaa ctgtggcact ttgttctcct
     1741 ttgggggatt gttctcggca tcgccaccgg cgtgacaccg gcattgtcgg cgagcgtggc
     1801 gagccgctgg tttatcgccc ggcgtggtct ggccctgggt attctcacca atgccaacgc
     1861 caccgggcag gtaattttct tgcctattct tatggcgcta atcgtctccc acggttggcg
     1921 cccggcgatg atgttgatcg tcggcacggc gctactcctg ttgccggcaa tctggttgtg
     1981 gctgcgcgac tatcctgcag agctcggcct cgaaccttat agcgatggca cgaccacatc
     2041 tgaccaacgc aatcaggcgc tcgccgcgcc tcgggtattt tccctggcct cattcggcga
     2101 cgcgattaag acgccgacgt tctggctcct gagcggcagt ttcttcgtct gcggcatgac
     2161 ctccaacggg ttagtcggca cgcatatgat tccccatgcc attgaacgcg gcatcgcgga
     2221 agtgacggcg gcgaccgcgg tagggatcat gggcgtggcg agctttgtcg gaacgacgtt
     2281 cgccggttgg ctgtgcgacc gagtggacgc gcgcaaagta ttgtcggcgg cttatttgtt
     2341 ccgtggtttg tcgctgtttg tcctgcccta cgtgaccgac tcgtccggct tattcgtctt
     2401 cgcggtttta tacggcttcg actggtttgc caccggtccg tcgaccgtgg cgctgatcgc
     2461 caaacacttt ggccaggatc gggtcggcgt gctcttcggc ttggttttcg tatcgcacca
     2521 gatcggcagc gccatcgccg cccttggcgg cggctggatc tacagcgagt ttggcgaata
     2581 ctactacgct tttatgagtg gcgcggtgat gggtttgctc gctgccggga tggcgatgat
     2641 gattcgccgg gaccgggatc aggccccggc tgtgcctgtg agcacagcag ccgtgcgcgc
     2701 gtgagccttt ttcgcttaac ccgaaaattc cagaaaaaca aaatagccaa tagtaaactg
     2761 tgtcgctcaa tcgcggtagc gcgcctgcat ctgggcgaaa aagccgctgt cgtccaattt
     2821 gcggataaac gagctgtcga tcaggctcgc cggattgacg tcggcgagtt tggggttttg
     2881 catcgccaaa agccgctgca tgttcttcaa cccttcgagc gacgggtagg gtttggcttc
     2941 gaactccttg gcaagatcgg tgtgtgcttc ttcggcggac gccgaatcgg tgagcctgag
     3001 atggctcatg atcgatttga tggtttgggc tttgccactc ggggcaaggg aaaaatactc
     3061 gccttcgatc agcgccttca ggacattttc cagtagcgcg ggatttttct cgagataatt
     3121 cttgcgtgta accataccgg tggcggccat cgcgggattg acttcggtca agacgttgaa
     3181 gcccttttgc agcagaccgg gaatgtaggt gcgcgtagtg agcacggtgg catcgatcac
     3241 ttgcgactcg agggcgcgaa cggtcacggt ttggtcgccg atgatgagca ggcgaatctt
     3301 gtcgcgcgtc aaatcgagac cgagttgttc gagtgcgagc gtgacgtaca tccaaagggt
     3361 cccaccgata ctttgaacgc cgatgcgttt gccggctaag tcggccggtt tcttgatatc
     3421 cgaccgaacc agcaggtggc ctttgccgcg gctgacgaag ttggcaatca tctttagttc
     3481 cgcgccggcc gcggccgcgc cgagaacggt
//
LOCUS       DASQKS010000218         6901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3054798, whole genome shotgun sequence.
ACCESSION   DASQKS010000218 DASQKS010000000
VERSION     DASQKS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054798"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VE170_05675"
     CDS             complement(<1..697)
                     /locus_tag="VE170_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14959.1"
                     /translation="MRLVTRADFDGLVCGALITKFENVDDFLYVEPKFMQDGLVEIRR
                     GDIIANLPYHPNCSLWFDHHITNTTPNFATPIILGRGGFRLAPSAARVVYEYYLEVAA
                     TGDSAVKAQYALPGHAAEIEQFLKSSRVQHLMHEVDRVDAGKLEQQDVLNPQGYVLLS
                     MTTDGRNAGDEPYWLKVINLLRDATLQEILNDPDIKRRCQRIQEEQEKLRQLLLQKTR
                     YKSNVIYCDLRGVE"
     gene            complement(694..1809)
                     /gene="ald"
                     /locus_tag="VE170_05680"
     CDS             complement(694..1809)
                     /gene="ald"
                     /locus_tag="VE170_05680"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYJ14960.1"
                     /translation="MIIGVPKEIKTEENRVAVTPTGVAGFVARNHQVLIEKGAGSGSG
                     LADANYQAAGATIVDTAKEVWDRADMIMKVKEPQESEFPLLRPGLMLFTYLHLAAAES
                     VTRALLDRKVTAIAYETIQLDDGSLPLLAPMSEIAGRLSIQVGAWCLQAENGGRGILL
                     GGASGVRPANVVIIGAGMSGTAACQVAAGMGAYVSILDINPTKLRYVHDILGGHVTTL
                     MSNRANVEEAVVGADLVIGSVLIPGAQAPKIITRALVRRMKPGAAFVDIAIDQGGCAE
                     TSRPTTHTNPIYVEEDLVHYCVTNMPAIVPNTSTYALTNSTLTYGLELANRGFPQALV
                     RNKALAKGLNTFNGKVTYEGVANALNLPFTPVDEVIR"
     gene            complement(1917..2282)
                     /locus_tag="VE170_05685"
     CDS             complement(1917..2282)
                     /locus_tag="VE170_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HYJ14961.1"
                     /translation="MDRFFFSAGAVAAFIAVALGAFGAHSLKSKISAEMLDIFEVGVR
                     YQMYHALALLAVAWATTRWPEVDLNSAGWCFIAGIVIFSGSLYLLSFTGVRWLGAITP
                     IGGLAFLVGWAILIWSLNR"
     gene            complement(2352..3821)
                     /locus_tag="VE170_05690"
     CDS             complement(2352..3821)
                     /locus_tag="VE170_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HYJ14962.1"
                     /translation="MTTDGIRFVDTTIRDGHQSLWAESMTTGMMLPVAEAMDRAGFDA
                     IELISSSHLKKCVRELKEDPWARVKLVSQRIKNTPLRLNAGRFSAFDITPRSMYRLFM
                     ERMAANGIREARISEEWNELEGWEWKTKVSRDVGINPVLNIIYSVSPKHTDDYFAERT
                     RQAASLKVNRLCLKDPGGLLTPERVQTLVPIVFANANGIPVELHTHCTTGLGPLCCLE
                     AVKLGIKIVNTALPPLADGSSNPSLFNVAKNLRALGYQTLIDEELLKPVSDHFTYIAK
                     REGFPIGAPVEYDYSQYQHQVPGGMISNLRFQLKKVGLENKIDQALEETMQVRAELGY
                     PIMVTPLSQFVGSQAAINVIVGERYKEVTDQIIQYALGYWGKEGAELMAPEVKAKILD
                     RPRAKEWAGRPPPEPSVAELRKKMHSENVSDEDFLLRWNLEVKEIEEMKAAGAPKEYL
                     TASQPLVKLIDALSQRKDYRQIVIQKGEMVVSLQRDAAT"
     gene            complement(3913..4770)
                     /locus_tag="VE170_05695"
     CDS             complement(3913..4770)
                     /locus_tag="VE170_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ14963.1"
                     /translation="MASDIVAIDVHVHLSDETTQKAKGDRTAQMARYFGRERPPVSID
                     EMADQYRARKMMAVIMNTRDETVTGLTPVPNDHVASAIKKHPDVFLAFGAIDPWTGKL
                     AIDEVKRCHDLGLRGMGELNPARQHFYPNDPRFYPIWEQCAKLNMSILFHGGMAAAGA
                     GTPGGMGIKLKYSQPIYVDEVAADFPELKIISAHPTWPWTAESLAVARHKGNYYIDLS
                     GWAPKYFPAELLHNINTLLQDKAMFGSDWPAIGVERWLEEFGQVNMKPEIRKKIMLDN
                     AVKFFGLTL"
     gene            complement(4920..6377)
                     /locus_tag="VE170_05700"
     CDS             complement(4920..6377)
                     /locus_tag="VE170_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HYJ14964.1"
                     /translation="METIRFVDTTIRDGHQSLWAENMTTGMMLPIAKNLDDAGFEGIE
                     LISGSHLKKSVRDLKEDPWERVRLVAKLITKTPLRLIAGRVNTFEYNPPSMYRLFVER
                     MFANGIKEARISDEWNDYEGWKFRVGECHRAGMKSILNLIYSVSPKHSDEYFALRTRQ
                     AASLKPYRLCLKDPGGLLTPERMETLVPIILKNANGIQVELHTHCTTGLGPLCCIKAM
                     ELGIRSINTALPPLADDTSNPSLYNVAKNARALGYQTDIDEEILQPVSRHFHAIAKRE
                     GFTIGAPVEYDYSQYQHQVPGGMLSNLRFQLRKVGLEHKFPAALEEAIRVREEFGYPI
                     MVTPLSQFVGSQAAINVIVGERYKEVTDQVIKFALGHAGAEGARDMATDVKDKILDRP
                     RAKEWAKWTPDDPSPDEIRRNIGAEGTSDEELLLRWIIGKEDIDFMRANKRPLEYLNS
                     EQPLVNLLQELSKRTKNRQIHVQTANFSVTMERRA"
     gene            6530..>6901
                     /locus_tag="VE170_05705"
     CDS             6530..>6901
                     /locus_tag="VE170_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYJ14965.1"
                     /translation="MIEIAQALLFDRDGKLLIYLRDDKPTIPFPNHWDLFGGHVEAGE
                     TPERALCRELREELGVSLDSWRLFRRYECLAGDVYPNTKFIYYAQIDCRPADLTLYEG
                     QRLAGIAAAQRRDYPFANVLGA"
BASE COUNT         1338 a         1925 c         2087 g         1551 t
ORIGIN      
        1 cttcgacgcc gcgcaggtcg cagtaaatca cgttgctttt ataacgggtc ttttgcagca
       61 gcaactggcg tagcttctct tgctcctctt ggatgcgctg gcagcgccgc ttgatgtcgg
      121 ggtcgttcaa gatttcttgc agcgtcgcgt cgcgcaatag attgatcacc ttcaaccagt
      181 agggctcgtc gccggcgttc ctgccgtcgg tggtcatcga caacagcacg tagccctgcg
      241 gattcaacac atcttgctgc tccaatttac cggcatcgac gcggtcgacc tcatgcatca
      301 ggtgttgcac gcgcgagctt ttaaggaatt gttcgatctc ggcggcgtga ccgggcagcg
      361 catactgcgc ctttacggct gaatcgcccg ttgctgcaac ttcgagataa tactcgtaga
      421 caacgcgcgc ggcgctggga gcgaggcgaa agccgccgcg gccgagaata atcggcgttg
      481 caaaattggg cgtggtgttg gtgatgtgat ggtcgaacca gaggctgcag ttgggatggt
      541 agggaagatt ggcaatgatg tcgccgcggc gaatctcgac caaaccatcc tgcatgaact
      601 tgggttcgac gtacaaaaag tcgtcgacat tttcaaactt ggtaatcagc gcgccacaga
      661 ctaggccgtc gaagtcggcg cgagtgacga gtctcatcga atcacctcgt cgacgggcgt
      721 gaagggtagg tttaacgcgt tggcgacacc ctcgtaggtg accttgccgt tgaaagtatt
      781 caagcctttg gccaatgctt tgtttctgac cagcgcttgg ggaaaaccgc ggttggcgag
      841 ttcgaggccg taggtcagag tcgaattggt tagcgcgtag gtcgaggtgt tgggcacgat
      901 cgctggcatg ttggtgacgc aatagtggac cagatcttcc tcgacataga tcggattggt
      961 atgggtcgtc ggccgcgaag tctcggcgca gccgccttga tcgatagcga tgtcgacaaa
     1021 ggccgccccc ggcttcattc gtctgaccaa cgcgcgcgta atgatcttcg gcgcctgggc
     1081 gccggggatc aagaccgagc cgatgaccag atcggcgccg accaccgctt cttcgacgtt
     1141 ggcgcggttc gacatgagcg tcgtgacatg gccgcccagg atgtcgtgga catagcgcag
     1201 cttggttgga ttgatatcga gaatgctgac ataggcgccc attccggcgg cgacctggca
     1261 ggccgcggtg ccggacatgc cggcgccgat gatgacgaca ttggccgggc gcacccccga
     1321 tgcgccgccg agcagaatgc cgcggccgcc gttctcggcc tgcaggcacc acgcgccgac
     1381 ttggatggac agccgcccgg cgatctcgct catgggcgca agcagcggca gcgaaccgtc
     1441 gtccagctgg atggtctcgt aggcgatggc ggtgacttta cggtcgagca gggcgcgagt
     1501 gacggactcg gcggcggcga gatggaggta agtaaatagc atcagacctg ggcgcagcag
     1561 aggaaactct gactcctggg gctctttgac cttcatgatc atgtcggccc tatcccagac
     1621 ttctttggcc gtgtcgacga tagtcgcgcc ggcggcttga taattggcgt cggcgagacc
     1681 gctgccgctg ccggcgccct tttcaatcaa cacttggtga ttgcgcgcga cgaagccggc
     1741 gacgccggtg ggtgtgaccg cgacccgatt ctcttcggtc ttaatctctt tcggtacacc
     1801 gataatcatg cggaaaagct acgccaagcg acgcaaaaag gcaaaagtta aaagcccgga
     1861 atgaaatgat ggagtgattg gaatgtcgga cggaaatcca cgaccccaat cgactgtcac
     1921 cgattcagcg accagatcag gatcgcccag ccgaccagga acgccaggcc gccgatgggg
     1981 gtgatggcgc caagccagcg cacgccggta aagctcagca gatacaggct gccggaaaaa
     2041 atcacgatac cggcgatgaa gcaccagccg gcggaattga ggtcgacttc gggccaacgg
     2101 gtagtcgccc acgcgaccgc caaaagcgcc agcgcgtggt acatctggta gcgcacaccg
     2161 acttcgaaga tgtcgagcat ctccgccgaa attttgcttt tgagtgagtg ggcgccgaag
     2221 gcgcccaggg cgacggcgat gaaagcggcg acggcgccgg cgctaaaaaa gaagcggtcc
     2281 atataaacct gttatcaaac gtgctcgcgg tgtgtgttta aaccagggca agacctgccg
     2341 acactggttg attatgtcgc cgcgtctcgt tgcagcgaaa ccaccatctc gcctttttgg
     2401 atgacgatct ggcggtagtc tttgcgttgg ctgagcgcgt cgatcaattt caccagaggc
     2461 tgactcgcag ttaaatattc cttgggcgcg ccggcggctt tcatttcttc gatttctttc
     2521 acttcgagat tccagcgtaa caggaaatct tcgtcggaaa cattctccga gtgcattttc
     2581 ttgcgcagtt cggcgaccga cggttcgggc ggcgggcgtc cggcccattc cttggcgcgt
     2641 ggccggtcga ggatcttcgc cttgacttcc ggcgccatca attcggcgcc ctccttgccc
     2701 caatagccga gcgcgtactg gatgatttga tcggtgactt ccttgtagcg ctcgccgacg
     2761 atgacattga tcgccgcctg actgccgacg aattgggaca gcggcgtgac catgatcggg
     2821 tagccgagct cggcgcgtac ttgcatggtt tcttcgagcg cctggtcgat cttgttttcc
     2881 aggccgactt ttttcagctg gaagcgcagg ttggaaatca tcccgccggg aacctggtgt
     2941 tgatactgcg agtagtcata ttccaccggc gcgccgatcg gaaagccttc gcgcttggcg
     3001 atataggtaa aatggtcgga caccggcttg agcagttctt catcgatcaa agtttggtag
     3061 ccgagcgcgc gcaggttctt ggcgacgttg aataacgatg gattcgacga gccgtcggcc
     3121 agcggcggca gcgcggtgtt gacgatcttg atgccgagct tgactgcttc gagacagcag
     3181 agcggaccca agccggtggt gcagtgggtg tgcagctcca ccggaatgcc attggcattg
     3241 gcgaaaacga tcggtaccag cgtctgcacc cgttccggcg tgagcaggcc gccgggatct
     3301 tttaggcaca agcggtttac cttcaacgac gcggcttggc gcgtgcgttc ggcaaagtag
     3361 tcatcggtgt gtttgggcga aaccgaatag atgatgttca gcaccggatt gatgccgaca
     3421 tcgcgggaga ctttggtttt ccactcccag ccttccagtt cgttccactc ttccgaaatg
     3481 cgcgcttcgc ggatgccgtt ggcggccatc cgttccatga acagccggta catcgagcgc
     3541 ggcgtgatat cgaaggcgct gaagcggccg gcgtttagac gcagcggcgt attcttgatg
     3601 cgctggctga ccagtttgac ccgcgcccat ggatcttctt tcagttcgcg cacgcacttc
     3661 ttcaaatgcg agctcgagat cagttcaatc gcgtcgaaac cggcgcgatc catggcttcg
     3721 gcgacgggca acatcatgcc ggtggtcatg ctttccgccc acaggctttg atggccgtcg
     3781 cggatggtgg tgtcgacgaa gcgaattccg tcagtggtca tgagggtttc ctggagtagt
     3841 ggagtgatgg agtactggaa agtttacggt ccatggttcc accactccaa tactccatca
     3901 ctccaaactt ctttacaggg ttaagccgaa aaacttaacg gcgttgtcga gcataatctt
     3961 cttgcggatc tcgggcttca tgttgacctg gccgaattct tccagccagc gctcgacgcc
     4021 gatggccggc cagtcggagc cgaacatcgc tttgtcttgc agcagcgtgt tgatgttgtg
     4081 cagcaactcc gccggaaaat attttggtgc ccagcccgac agatcgatgt aatagttgcc
     4141 cttgtggcgc gcgaccgcca agctttccgc ggtccatggc caggtcggat gggcgctgat
     4201 gattttcaac tcgggaaaat ccgccgcgac ttcgtcgacg taaatgggct ggctgtattt
     4261 caatttgatg cccatgccgc ccggcgtgcc ggcgccggcc gccgccatgc cgccgtgaaa
     4321 caggatcgac atattcaact tggcgcattg ctcccagatc gggtagaagc gcggatcgtt
     4381 ggggtagaag tgttggcgcg ccggattgag ctcgcccatg ccgcgcagcc ccaagtcatg
     4441 gcagcgtttg acttcgtcga tcgccaattt gccggtccag ggatcgatgg cgccgaaagc
     4501 gaggaagacg tcgggatgtt ttttgatcgc gctggcgaca tggtcgttgg gcaccggtgt
     4561 cagaccggtg acggtctcgt cgcgagtgtt catgatcacc gccatcattt tccgcgcccg
     4621 gtattgatcg gccatttcat cgatggaaac cggcggccgt tcgcggccga aataacgcgc
     4681 catctgcgcg gtgcggtcgc ctttggcttt ctgggtggtt tcatcggaca gatggacatg
     4741 aacgtcgata gctactatgt cgcttgccat ggtttagagc ctcctgagta agtttctttt
     4801 cttattgcac cggcgacggt ttagttcaag gttcaaagtt caatcgttca aggttgtcgg
     4861 attgcggaat cccaaccgtt gaacctttga accttgaact gttgaacgga atacagcgct
     4921 caagcacgcc gttccatggt caccgagaaa ttcgccgtct gcacatggat ctgccggttc
     4981 ttggtgcgtt tggacaattc ttgcaacaag ttcaccaacg gctgttccga attcaaatat
     5041 tccagcggcc gcttgttggc gcgcatgaag tcaatatcct ctttaccgat gatccagcgc
     5101 aagagtaatt cttcgtcgga ggtgccctcg gcgccgatgt tgcggcgaat ctcgtctggc
     5161 gaggggtcgt cgggtgtcca cttggcccat tccttggcgc gcggccgatc gagaatcttg
     5221 tccttcacat cggtcgccat gtcgcgtgcg ccctcggcgc cggcatggcc cagggcgaac
     5281 ttgatcacct ggtcggtgac ttctttgtag cgctcgccga caatcacatt gatcgccgcc
     5341 tgactgccga cgaactgcga cagcggcgtg accatgatcg gatagccgaa ttcctcgcgc
     5401 accctgatcg cttcttccag cgccgcggga aacttgtgtt ccaggccgac cttgcgcagt
     5461 tgaaaccgca ggttcgacag catgccgccg ggcacttggt gttggtattg cgagtagtcg
     5521 tattccaccg gcgcgccgat ggtaaagccc tcgcgcttgg cgatggcgtg gaagtggcgc
     5581 gataccggct gcaaaatttc ttcgtcgatg tcggtctggt agccgagggc gcgggcgttt
     5641 ttggcgacat tatataaaga cggattcgaa gtgtcgtcgg ccagcggcgg cagcgcggta
     5701 ttgatcgagc ggatgcccaa ctccatggct ttgatgcagc agagcggccc caagccggtg
     5761 gtgcagtggg tatgcagctc gacttggatc ccgttggcgt tcttcaggat gatcggcacc
     5821 aaggtttcca tgcgctccgg tgtcagcaaa ccgcccgggt ctttcaggca aagccgataa
     5881 ggtttgagtg atgccgcctg gcgggtgcgc agagcgaaat attcgtcgct atgtttgggc
     5941 gacaccgaat agatcaagtt tagaatcgac ttcatgccgg cgcgatgaca ttcgccgacg
     6001 cgaaatttcc atccttcgta gtcgttccat tcatccgaga tgcgcgcttc cttgatgccg
     6061 ttggcgaaca tgcgctcgac gaagagccgg tacatggacg gcggattgta ttcaaaggta
     6121 ttaacccgcc cggcgatcag gcgcagcggc gttttggtga ttaatttcgc gaccagccgc
     6181 acgcgctccc aagggtcttc tttcaggtcg cgcacgcttt tcttcaagtg cgagccggaa
     6241 atcaactcga tcccttcaaa ccccgcgtca tccaagttct tggcgatcgg cagcatcatg
     6301 ccggtggtca tgttttccgc ccacaagctc tgatggccgt cgcggatggt ggtgtcgacg
     6361 aatctaatgg tttccatggc gtctcccaat aactgccgtt gcagtcgtta ttacagattc
     6421 gtgacataga tcaagacact cggcgatcag cgggaggctt agcacggcac gagcgccgcc
     6481 ttcggccgac ggctttagat tcccccaccg atggtctaca ttccacgcga tgattgaaat
     6541 cgcccaagcg ctgctgtttg accgggatgg caagctgttg atttacctgc gcgacgacaa
     6601 accgacgatt ccgtttccca accattggga tttattcggc ggtcatgtcg aagcggggga
     6661 gacgccggaa cgggcgctgt gccgggagct acgggaagaa ctcggcgtga gcctcgatag
     6721 ttggcggctg tttcgccgtt atgaatgtct ggccggcgac gtttatccca atactaagtt
     6781 tatttactac gcgcagatcg actgtcgccc ggccgatttg acactttacg aaggtcagcg
     6841 attggccggc attgccgcgg ctcagcggcg cgattatcca tttgccaatg tcctcggcgc
     6901 g
//
LOCUS       DASQKS010000219         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2647278, whole genome shotgun sequence.
ACCESSION   DASQKS010000219 DASQKS010000000
VERSION     DASQKS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647278"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="miaA"
                     /locus_tag="VE170_05710"
     CDS             <1..743
                     /gene="miaA"
                     /locus_tag="VE170_05710"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYJ14966.1"
                     /translation="HHLIDVVNPDQEFNAATFRLLASEVIYQIDERQRRAIVCGGTGL
                     YLKALTRGLFEGAGQNGEIRRDLETEIAKTGLDSVYRRLVAIDPTVTSTIHPNDRSRT
                     VRALEVYQLTGKPISQWQKEHRFQEESFAVLKLGLNLERAELYERINRRSAAMIEAGF
                     LDEVRGLMARGYSLDLKPLRSIGYAQMGRVIRGALTIDAAREEMQQETRRLAKRQLTW
                     FRGDKQVHWFHPTQVAEILKTLKQFFAS"
     gene            763..2169
                     /locus_tag="VE170_05715"
     CDS             763..2169
                     /locus_tag="VE170_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ14967.1"
                     /translation="MKKFLNSILSILAIIFTWATAGIGADAKFDWKALEDEAVAILSR
                     YVQIDTTNPPGNELKAAAFLKAILDKEGIDARVIESAPGRANLYARLKGSGAKKAILL
                     MHHMDVVPAQADLWREPPFSGVLKDGAIWGRGTLDNKGGGVMALLTLVALKRQNIPLK
                     GDVIFLGTADEEAGGVHGAGFLVEKHPELFKDVSVVFNEGGGIRVGDDGRARLYSVGV
                     AEKVPLWLKLTAPGTPGHAASPGDNQAVLKLVAALNRLAAYQSPVKVTPEVQKFYADS
                     AATAPADRRKQYLDLRTALQEPTFAAEFLKDRSNNARVRNTISITGIKGSDKINVIPA
                     EASAEVDVRLLPGEVPQVFINELRRVLADDSIKIEILLSRTAATSPTSPEAMKVITEY
                     AKTHDPGAAVLFPVGSGFTDCHFFRVKGIPCLGFLPQRSTPSSEGLVHGVDERMPVER
                     LTAAIHAMYEIVSKLVVE"
BASE COUNT          487 a          583 c          674 g          448 t
ORIGIN      
        1 gccatcatct gatcgatgtg gtgaatcccg accaggagtt caatgccgcg acctttcgtc
       61 tgctcgcgag cgaagtgatc taccagatcg acgagcgcca gcgacgcgcg attgtctgcg
      121 gcggcacggg actctatctc aaggcgttga cccgaggatt gttcgagggg gcaggacaga
      181 atggggaaat tcgccgcgac ttggaaacgg aaatcgccaa gaccggtttg gattcggtct
      241 atcggcgact ggttgcgatc gatccgacgg taacctcgac gattcatccc aacgatcgtt
      301 cccgcacggt ccgtgctctc gaggtttatc agttgaccgg caagccgatt agccaatggc
      361 agaaagagca tcgtttccaa gaagagtcct tcgcagtgct gaagctggga ttaaatcttg
      421 agcgcgccga gctctacgag cgcatcaacc ggcgcagtgc ggcaatgatc gaagcgggtt
      481 tcctcgatga agtgcgcggc ctgatggcgc gcggttactc cctcgatcta aaaccgctgc
      541 gcagtatcgg ctatgcgcag atgggcaggg tgattcgcgg cgctttaacg atagacgcgg
      601 cgcgggagga aatgcagcag gaaactcgcc gcttggccaa gcgccaattg acctggttcc
      661 gcggcgataa acaagtgcat tggtttcatc ccacgcaggt cgcggagatt ctcaagacct
      721 tgaagcagtt ctttgcaagc taatgtgcga ttggaggaga ggatgaaaaa gttccttaat
      781 agcattctca gcatattggc gatcattttc acctgggcaa ccgccggaat tggcgccgat
      841 gccaaattcg actggaaggc tctcgaagac gaagcggtag cgattctaag ccgctatgtt
      901 cagatcgaca cgaccaatcc gcctggcaac gagttgaaag cagcggcgtt tctcaaagcg
      961 atcttggata aggaaggcat cgatgcgcgc gtcatcgagt cggcgccggg ccgggcaaac
     1021 ttatatgctc ggctcaaagg tagcggggct aaaaaggcga ttctgttgat gcatcacatg
     1081 gacgtcgtgc cggcacaagc cgatctatgg cgcgagccgc cattcagcgg tgtgcttaaa
     1141 gacggcgcga tttggggccg gggtacgctc gataacaagg gcggcggtgt gatggcattg
     1201 ctgactctgg tcgctctcaa acgacaaaac atcccgctca agggcgatgt gatttttctc
     1261 ggcaccgccg acgaggaggc gggaggcgtg cacggcgcag gcttcttggt cgaaaaacat
     1321 ccagagctgt ttaaagatgt cagcgtggtg tttaacgagg gcggtggtat ccgcgtcggc
     1381 gacgacggcc gcgcgcgttt gtatagcgtc ggcgtggcgg aaaaagtacc gctttggttg
     1441 aagcttacgg cgccaggaac gccgggtcat gcggcgtcac cgggagataa tcaggcggtg
     1501 ctcaaattgg tcgccgcgct caatcgcttg gcggcctatc agagtccggt caaagtgacc
     1561 cccgaagtgc agaagtttta cgccgatagc gcggcgacgg ctccagccga ccgacgcaaa
     1621 caatacttgg acctgcgcac ggccctgcag gagccgacgt ttgccgctga gtttctcaaa
     1681 gaccgtagca acaatgcccg ggtgcgcaat acgatttcca tcaccggcat caaaggctcc
     1741 gacaagatca acgtgatccc tgccgaagct tcggcggaag tcgacgtgcg cttattgccc
     1801 ggcgaagtgc cgcaggtgtt tatcaacgaa ttgcgccgtg tgctcgccga cgattcgatc
     1861 aagatcgaaa ttttgctctc gcgcacggcg gcgacctcgc cgacgagtcc ggaagcgatg
     1921 aaagttatca ccgagtacgc taaaactcac gatcccggcg cggccgtatt atttccggtt
     1981 ggcagcggct ttaccgattg ccactttttt cgcgttaaag gaattccctg tctaggtttc
     2041 ctaccgcagc gcagtacgcc gagcagcgaa gggttggtgc acggcgtcga cgagcgtatg
     2101 ccggtggagc gtctgacagc ggcgattcac gcgatgtacg agatcgtcag taagttagtg
     2161 gtagagtgag ctaatgaaac gggcccatta ct
//
LOCUS       DASQKS010000220         6164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2218413, whole genome shotgun sequence.
ACCESSION   DASQKS010000220 DASQKS010000000
VERSION     DASQKS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218413"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VE170_05720"
     CDS             complement(<1..1028)
                     /locus_tag="VE170_05720"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYJ14968.1"
                     /translation="MMSVLGVGDLTLLLAIPVIGAALLVLVPRRQSTGLFAIALLASA
                     AAFLWSLKIFDRFDGANGEMQLVERAAWMPAIGVQYILGIDGISIFLVLLTTFLMPLA
                     LLASWSVRDKVKEYLIFMLLLETGMLGAFLALDLVLFYVFWEVMLVPMYFLIGVWGGT
                     RRIYAAVKFVLFTMAGSLLMLVAIIYLATRHAQINQVLTFNVLQLYDLHLPVAQQTWL
                     FLAFALSFAVKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKLGTYGFLRFAMPLFPD
                     AAIALMPYLVVLSVIGIVYGAMVAMMQSDIKKLVAYSSVSHLGFVMLGLCALNLPGVQ
                     GSIYQMLS"
     gene            complement(1025..2950)
                     /gene="nuoL"
                     /locus_tag="VE170_05725"
     CDS             complement(1025..2950)
                     /gene="nuoL"
                     /locus_tag="VE170_05725"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYJ14969.1"
                     /translation="MVETGQNLLSSELLRWIPLIPLLGSVINLFFGRALGKQTAGALA
                     SAAVGASFALAFYIFWQLPATGIFRDLVYTWIQSGSFKVNLAFQVDALTAVMLLVVTG
                     IGFLIHLYSLGYMGHDPDMVRFFIYLNLFIFFMLVLVMGDNLLLLFVGWEGVGLCSYL
                     LIGFWYHDHNNTIAANKAFIVNRIGDWGFILGIFVLLTELGRQGIWTLDFAELAKHVK
                     LMQPGTITLITLLLFIGAAGKSAQLPLFVWLPDAMAGPTPVSALIHAATMVTAGVYMT
                     ARLHFLFALAPATLAVIAVIGAATAFFAATIALTQNDIKKVLAYSTVSQLGYMFLAVG
                     MGAFGAAVFHLFTHAFFKACLFLGSGSVIHALGGEQDMRKMGGLKTHMPQTYWTYVVA
                     TLAIAGVPFTAGFFSKDLILWQTFSHGAGILWGVGLITAGMTAFYMFRQLFMVFHGEC
                     RADDHAKAHLHESPAVMTWPLIILAVGSIFTGWLGAPEYLWGSRWNHWLAPIFGAATE
                     PHGDLGSELLVTSLTLAVVAAGIYLAYLMHGKPGAQGAQPAAASIFYDVSLNKYYVDE
                     IYDFIFVRPFTALARFFAGIVDPWVIDGAVNGVAATARGFSTVWRGLQTGNVQHYAAM
                     FLVGALALLAYYLGQLS"
     gene            complement(2944..3246)
                     /gene="nuoK"
                     /locus_tag="VE170_05730"
     CDS             complement(2944..3246)
                     /gene="nuoK"
                     /locus_tag="VE170_05730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYJ14970.1"
                     /translation="MIALNYYLILSAVVFAIGVIGVLIRRNLIVILMSIELMLNAVNL
                     TFIAFSRYLGSTDGQVAVFFVMAVAAAEAVIGLAIIISVYRHRQTLDPQEMQLLKW"
     gene            complement(3252..3746)
                     /locus_tag="VE170_05735"
     CDS             complement(3252..3746)
                     /locus_tag="VE170_05735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYJ14971.1"
                     /translation="MDTASLIFYLLATLLVIASLLVVFLRNVVHCALALVAALLLIAL
                     LFVTLQAAMVGVLQVLVYAGAIMVLFLFVIMLLNPAVLERQRGLWWGFGTMLALLLAG
                     VLVALVMTNDLPADPVAATELFGSPEMLAKSLFSDFVLPFEIASVLLLVAIIGAVVLA
                     KREH"
     gene            complement(3758..4888)
                     /locus_tag="VE170_05740"
     CDS             complement(3758..4888)
                     /locus_tag="VE170_05740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HYJ14972.1"
                     /translation="MIVDLAIITLKAALVVGMVLNLAGVLGWIERKGSALIQDRVGAN
                     RASILGFAGLGLVNTLIADPLKFLFKEDFIPPTGDRFLHTLAPCMALFPALVTFAVIP
                     FGDVLNIGERSINLQVASLNIGILYVFAMGSLGVYGIVIGAWASNNKFAMLGGVRGSA
                     QMISYEIAMGLSVMGVLMVYGTLELQDIARGQGALLRDLMPSWLSFISPIVGWLPAWG
                     IFLQPLAFLLFFTAAVAETKRIPFDLPEGESELVAGYHVEYSGGKFLMFFAGEFAEVV
                     TVAGLVTTLFFGGWQVPYLLRDGFHFPWGGTWLLPHVLIVLLQVTAFTVKVVFFCWLQ
                     ILLRWSVPRFRYDQVMRLGWKMLLPLALLNVVATAIWIVAAQ"
     gene            complement(4885..>6164)
                     /locus_tag="VE170_05745"
     CDS             complement(4885..>6164)
                     /locus_tag="VE170_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYJ14973.1"
                     /translation="AGECQLQNYYMEHDRQESRLEVGKIHDRKKTIFGPNVVFDGERC
                     IKCTRCVRFCQEITKTHELAVVNRGDHSTIALFDDAVLDNPLSANVVDICPVGALTDR
                     DFRFKVRVWYLDKTPSICPGCSTGCNISVEAYQNKIARFKPRQNEAVNSHWICDEGRY
                     CFHDLAGGERLTTPLIRQEGGVLVPTTWETALQAVVNGLRGAQPLAGILSGRNTNEEA
                     YLFAKLIPKLANDCAMEVFYQERQLSEVQKLLMSPDRSPNFRGARDMGVSSSRGFAGL
                     VQELVGNHFSSAFIVGEDLLGANGNSAEIRSALEKLSFLVVQDTKRTATAELAHVVLP
                     AAHFGEKEGTYTNRKGRVQKLNAAVICPEGARQDSEIFLSLLDGAGQALDYATPSDIF
                     TALTQEVPTYKGLTYDTIGAQGFELGSGGGAAS"
BASE COUNT         1573 a         1809 c         1751 g         1031 t
ORIGIN      
        1 tggctgagca tttgatagat gctaccctgc actcctggca ggttgagggc acagagcccg
       61 agcataacga agcccaaatg gctcaccgac gaataggcga cgagcttttt aatgtcgctc
      121 tgcatcatcg ccaccatcgc gccgtagacg atgccgatga ccgatagaac gacgaggtag
      181 ggcatcagcg cgatcgccgc gtcgggaaaa agcggcatgg cgaagcgcaa aaagccatag
      241 gtgccgagct tcagcagcac cccggcaagg atcaccgatc cggcggtggg tgcctccacg
      301 tgggcgtcgg gcagccaggt atgaaacggg aaaagcggga ccttgacggc gaacgataac
      361 gcgaaggcga ggaacagcca agtttgctgc gcaaccggca gatgcaggtc atagagctgc
      421 aggacattga aggtcagcac ctggttgatc tgagcgtggc gcgtggccag ataaataatc
      481 gccaccagca tgagcaagct gccggccatg gtgaacagca cgaatttcac cgccgcatag
      541 atgcgccgcg tgccgcccca cacaccgatc aaaaaataca tcggcaccag catcacttcc
      601 cagaaaacat agaacagcac cagatcgaga gccaggaatg cgccaagcat gccggtttcc
      661 agcaacaaca taaaaatcaa atactctttg accttgtcgc gcaccgacca ggaagcgagg
      721 agcgccagcg gcatgagaaa agtcgtgagc aaaaccaaaa aaatactgat gccgtcgatg
      781 cccaggatat attgcacgcc aatggccggc atccacgccg ctcgctcgac cagctgcatc
      841 tcgccattgg cgccgtcgaa tcggtcgaaa attttgagcg accagagaaa cgccgccgcg
      901 ctggcgagca atgcgatggc gaatagtccc gtcgactggc gccgcggcac caacactagc
      961 agcgccgcgc cgatcaccgg gatggcgagg agcagggtca aatctccgac gccgagaact
     1021 gacatcatga caattgcccg aggtagtagg cgagcagcgc cagggcgccg accaaaaaca
     1081 tcgccgcgta gtgctgtacg ttgccggtct gcaagccgcg ccagacggtg ctaaaaccgc
     1141 gcgccgtggc ggctacgcca ttgactgcgc catcgatcac ccagggatcg acgatgccgg
     1201 cgaaaaatct ggccagggca gtgaacggac gaacaaaaat aaaatcgtaa atttcgtcga
     1261 cgtagtattt attgagcgaa acatcgtaaa aaatgctggc ggcagccggc tgcgcgccct
     1321 gcgccccagg cttaccgtgc atgagatagg cgagatatat tcccgcggcg accacggcca
     1381 acgtcaggga ggtcactaat aattcgctac cgagatcgcc atgaggttcc gtggccgcgc
     1441 cgaagatcgg cgccagccaa tggttccaac ggctgcccca gagatattcc ggcgcgccca
     1501 accagccggt aaagatcgac cccacggcaa gaatgatcag cggccaagtc atcaccgccg
     1561 gcgattcatg cagatgcgcc ttggcatggt catcggcacg gcactcgcca tgaaagacca
     1621 tgaaaagttg acgaaacatg taaaacgcgg tcatcccagc ggtgattagg ccgacgcccc
     1681 ataggatgcc cgcgccgtgg ctgaaggtct gccagagaat caaatctttg gagaaaaaac
     1741 cggcggtaaa aggcactccg gcgatcgcca gcgtggcgac gacgtaggtc cagtaggtct
     1801 ggggcatatg cgtcttgagg ccgcccatct tgcgcatatc ctgctcgccg ccgagggcgt
     1861 gaatcacgct gcccgaaccc aagaacaggc atgctttgaa aaatgcgtgg gtgaagagat
     1921 gaaagaccgc cgcgccaaag gcgcccatgc cgacggcgag aaacatgtaa cccaactggc
     1981 tcaccgtcga gtaggccaga actttcttga tatcgttctg ggtcagggca atggtggcgg
     2041 cgaaaaacgc cgtcgccgcg ccgatcacgg cgatcaccgc caaagtcgcc ggcgccaggg
     2101 cgaagagaaa atgcagccgc gccgtcatat agacgccggc ggtgaccatg gtggcggcgt
     2161 gaatcaacgc gctcaccggc gtcggcccgg ccatggcatc gggcagccac acgaacagcg
     2221 gtagctgtgc cgatttacca gcggcaccga tgaaaagcag caaggtaatc aaggtaatcg
     2281 tgccgggctg cataagcttc acgtgcttgg ccaattcggc gaagtccaag gtccagatgc
     2341 cttgacgccc taactcggtg agcaagacga aaatgccgag gatgaaaccc cagtcgccaa
     2401 tgcggttgac gataaacgcc ttattggcag cgatggtatt gttgtggtcg tggtaccaga
     2461 agccgattaa aagataagag cagaggccga cgccctccca gccgacaaac agcaagagca
     2521 gattgtcgcc catgaccagc acaagcataa aaaaaataaa caggttgaga tagataaaaa
     2581 aacgcaccat gtcagggtca tggcccatgt agcccaagga atagagatgg atgagaaaac
     2641 cgatgccggt gacgaccagc agcatcaccg ccgtcaaagc gtcgacttga aaggcgagat
     2701 tgaccttaaa ggaacccgac tgaatccagg tataaaccag atcgcgaaaa attcccgttg
     2761 ccggcagctg ccaaaatatg tagaaagcca gcgcgaacga tgcgcccacc gccgccgacg
     2821 ccagagcgcc ggcagtttgc ttgcccaggg cgcggccaaa gaacaaattg atcacgctgc
     2881 ccaacagcgg aatcaacgga atccagcgca acagctcgga gctcaacaga ttttgaccag
     2941 tctctaccat ttgagcagct gcatctcctg gggatccaaa gtttggcgat ggcgataaac
     3001 ggatatgatg atggccagac cgatcacagc ttccgccgcg gccaccgcca tgacgaaaaa
     3061 taccgccact tggccgtcgg tcgagcccag atagcgtgag aaggcgatga aagtcaggtt
     3121 gacggcgttg agcatgagct cgatggacat gagaatgacg atcagattgc gccggatcaa
     3181 cacgccgatg acgccgatgg cgaagaccac cgcgcttagt atcagatagt aattcagcgc
     3241 aatcatggaa ctcaatgttc tcgcttggcc aataccaccg cgccgatgat cgccaccagt
     3301 agcaacaccg aggcgatttc gaagggcaga acaaaatcac tgaacaaact tttagccaac
     3361 atttcgggac tgccaaacaa ctccgtggcc gccaccggat cagccggcaa atcgttagtc
     3421 atcaccaacg cgaccaatac accggccaac agcagcgcca gcattgtgcc gaagccccac
     3481 cataggccgc gctggcgctc gaggactgcc gggttgagca acatgatgac gaaaagaaac
     3541 aggaccataa tagcgccggc atagaccagc acttgcaaca ccccgaccat cgccgcctgc
     3601 aaagtgacga acaacaacgc gatcaagagc agcgccgcaa ccagcgccag ggcgcagtgc
     3661 acgacgttgc gcaaaaacac caccagcagc gaagcgatga ccagcaaggt ggccaacaaa
     3721 tagaaaatca gacttgcggt atccatggat tcaaccgtta ctgtgccgcg acaatccaga
     3781 tcgccgtcgc gaccacgttg agaagcgcca gcggtaaaag cattttccag cccaagcgca
     3841 tgacctggtc gtaacgaaag cgcggcacac tccagcgtaa gaggatctgt agccagcaga
     3901 aaaaaaccac tttgacggtg aacgcggtca cctgcagcag gacgataagg acatgcggca
     3961 gcagccaggt gccgccccag gggaaatgga aaccgtcgcg caaaagatag ggcacctgcc
     4021 aaccgccgaa gaacagcgtc gtcaccaaac ccgcgacggt gacaacctcg gcgaactcac
     4081 cggcaaaaaa catcaagaac ttaccgccgg aatattcgac atgatagccg gcaaccaact
     4141 ccgactcgcc ttcgggcaag tcgaacggga ttcgtttggt ctctgccacc gcggcggtga
     4201 aaaaaagcag aaaggcgagg ggctggagaa aaattcccca ggccggtagc cagccgacga
     4261 ttggacttat aaaactgagc cagctgggca tgagatcgcg caacaaggcg ccctggccgc
     4321 gggcgatatc ttgcagctcc aacgtgccgt aaaccatcaa aacccccatg acggataatc
     4381 ccatggcgat ttcgtaggag atcatttgtg ccgagccgcg cacgccgccg agcatggcaa
     4441 acttattgtt cgacgcccaa gcgccgatga cgatgccgta gacgccgaga gagcccatgg
     4501 caaagacgta taatatgccg atgttgagac tggccacttg caggttgatc gagcgctcgc
     4561 cgatgttgag gacatcgcca aacggaatca ccgcgaaggt gaccagggcc gggaacaacg
     4621 ccatgcacgg tgctagggtg tgcaagaagc ggtcgccggt gggcggaatg aagtcttcct
     4681 taaaaagaaa cttgagcgga tcggcgatca gcgtattgac gagacccaga ccggcgaaac
     4741 ctaaaatcga tgcccggttg gcgccaaccc ggtcttggat cagcgcactg cccttgcgct
     4801 cgatccagcc tagaacaccg gcgaggttga gcaccatgcc caccaccagc gcggctttaa
     4861 gggtgataat cgcgaggtcg acgatcatga cgctgcgcca ccgccgcttc ccaactcaaa
     4921 tccttgagcg ccgatcgtgt cgtaggtcag tcccttgtag gtgggcactt cttgggtcaa
     4981 cgccgtaaaa atatcgctgg gcgtggcgta atcgagtgct tgtccggcgc catcgagtaa
     5041 gcttaaaaaa atctcactgt cctggcgcgc cccttcaggg cagatcaccg cggcattgag
     5101 cttttgcacc cggcctttgc gattggtgta ggtcccctct ttctcgccaa aatgcgccgc
     5161 cggcaagacc acatgggcca actcggcggt tgccgtccgc ttggtatcct gcaccaccag
     5221 gaaactgagt ttctccaagg cgctgcgaat ctccgcgctg ttgccgttgg ctccgagcaa
     5281 atcctcgccg acgatgaagg cgctggaaaa gtgattgccg actaattctt gcaccagacc
     5341 ggcgaaaccg cgactcgagc ttactcccat atcgcgggcg ccgcggaagt ttggcgagcg
     5401 gtccgggctc ataagcagct tttgcacttc gctgagctgg cgttcctggt agaacacttc
     5461 catggcacaa tcgttggcga gcttgggtat gagcttggcg aacaagtacg cctcttcgtt
     5521 ggtattgcgc ccggagagaa ttcccgccag cggctgcgcg ccgcgcagtc cattgaccac
     5581 ggcttgcagc gccgtctccc aggttgtcgg caccaatact ccgccctcct ggcgaattag
     5641 cggcgtggtc aggcgctcgc cgccggcgag atcgtgaaaa cagtaacgcc cctcgtcgca
     5701 gatccagtgg ctgttgaccg cctcgttctg acgcggttta aagcgcgcga ttttgttttg
     5761 ataggcctcg acgctgatgt tgcagccggt gctgcaaccc ggacagatcg acggggtctt
     5821 gtctaaatac cagacccgca ccttgaaacg aaaatcccgg tccgtaagcg cgccaaccgg
     5881 gcaaatgtcg acgacgttgg cggataacgg gttatcgagc acggcgtcgt cgaatagcgc
     5941 tatcgtcgaa tgatcgccgc ggttgaccac cgccagttca tgggtcttgg tgatctcctg
     6001 gcaaaagcgc acgcaacgcg tgcacttgat gcaacgctcg ccgtcgaata ccacattggg
     6061 gccgaatatg gtcttcttgc ggtcgtggat cttgccgact tcaaggcggc tctcctgacg
     6121 atcatgctcc atgtagtaat tctgtaactg gcactcgccg gcct
//
LOCUS       DASQKS010000221         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3056232, whole genome shotgun sequence.
ACCESSION   DASQKS010000221 DASQKS010000000
VERSION     DASQKS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056232"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VE170_05750"
     CDS             <1..680
                     /locus_tag="VE170_05750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ14974.1"
                     /translation="GAHVVIADLDGVGGEATAKAILDAGGSAWARTTDVRSLPDLQGL
                     MHETVKNFDRVDVLVNNAAIYVTQKLWKGSVEDLELDEWDRVIEVNLKGVFLCSKAVI
                     PIMKQQRSGKIINIASGTFFSGSGNMPHYTTAKGGVVALTRVMARQLGEYGINVNCMT
                     PGSTMSEENVSPEVLKRREGSMDKRAFRRVETPADIVGTALFLASSDSDFVTGQLLVV
                     EGGGVMH"
     gene            complement(733..1245)
                     /locus_tag="VE170_05755"
     CDS             complement(733..1245)
                     /locus_tag="VE170_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HYJ14975.1"
                     /translation="MDTSVDYYSILGVKPGATVDDIKRAYRQMVFRFHPDRNPDSPEA
                     ADKFTQVRDAYAVLSDALKRRVYDSVKHPAGAEESDDAVEDEASDKEQSASSRSESRA
                     EPKFSQDFKQKIEPEPKCPSCAVVGIDHLVSRKGRSVSAHGKHFVLAPFNVVLCNACG
                     HVYGITGNSN"
     gene            complement(1305..1814)
                     /locus_tag="VE170_05760"
     CDS             complement(1305..1814)
                     /locus_tag="VE170_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14976.1"
                     /translation="MITKRRVATRVNPAKIDYATALRSIGQDLDRRGLKSFDIRFEGR
                     EYVALCGYQEPPAATPVELRYRPSDIDESDRAGENQRGKVAAPKDFLNQPQVLRAIGE
                     FLDRNEAKLVRLSNNDRRTKESTFIVEYISRDGERVIDDRTGAAIYDLCVGMYKQRGR
                     LTGNGGRRL"
     gene            complement(1964..2581)
                     /gene="nthA"
                     /locus_tag="VE170_05765"
     CDS             complement(1964..2581)
                     /gene="nthA"
                     /locus_tag="VE170_05765"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HYJ14977.1"
                     /translation="MSDDHGHDHPKHELSYHAKRIYAMRDLLVEKGVITEKDIQSQIE
                     YQEARTPANGANLVAKAWVDPQFKARLIADPKAACAEIGIDATALNEFVVLENTPQVR
                     HMVVCTLCSCYPRPILGRPPDWYKSFNYRQRSVSDPRGVMREFGLELTDDVEVRVHDS
                     TADIRYIVMPLRPAGTEGWSEADLAKLVTRDSMIGVMDALPMLPE"
     gene            complement(2594..2887)
                     /locus_tag="VE170_05770"
     CDS             complement(2594..2887)
                     /locus_tag="VE170_05770"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HYJ14978.1"
                     /translation="MTPATGRFRTGDRVKVKFEDRPGHLRTPWYIRGKSGRIEKVYGD
                     FLNPELLAFGKDGLPKKTLYQVAFDHSEVFGKAQGAKDKVLVDIYDHWLETLA"
     gene            complement(2884..3195)
                     /locus_tag="VE170_05775"
     CDS             complement(2884..3195)
                     /locus_tag="VE170_05775"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase"
                     /protein_id="HYJ14979.1"
                     /translation="MARGHHDVGGLPGAGAIEVTEHQLSDWEILADAVNQALGARGIK
                     RTDEMRRAREEMDSAAYRDMTYYERWIASIETILIEKNILTKAEIDRKLVEFESKWGE
                     P"
     gene            complement(3211..>3736)
                     /locus_tag="VE170_05780"
     CDS             complement(3211..>3736)
                     /locus_tag="VE170_05780"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HYJ14980.1"
                     /translation="FNWQHGTFLGATLASETTAAATGAVGVVRRDPMAMLPFCGYNMG
                     DYWAHWLAMGKKAANPPKIFRVNWFQRNERGRFLWPGFGENLRVLRWVVERSKGGGAA
                     DETAIGFVPKASSLTGDGLDIAKADVDQLVSVDREAWKNNLKSQSDFFDTFGDHLPAG
                     MKEEHGALANRLKK"
BASE COUNT          759 a         1096 c         1072 g          809 t
ORIGIN      
        1 aaggcgctca cgtggtgatc gccgacctcg acggggtcgg cggcgaagcg acggcgaaag
       61 cgatcctcga cgccggcggc tcggcctggg cgcggacaac agacgtgcgc agcctgcccg
      121 acctccaagg gctcatgcac gagaccgtga aaaatttcga ccgggtcgac gtcctggtca
      181 acaatgcggc gatttatgtc acgcagaaac tttggaaagg ttcggtcgag gacttggagc
      241 tcgacgaatg ggatcgcgtc attgaagtca atttgaaagg cgtgttcctg tgcagcaagg
      301 cggttatccc gatcatgaag cagcagcgct ccggcaaaat cataaacatc gcctcgggga
      361 ctttttttag cggctccggc aacatgccgc attacaccac cgccaaaggc ggggtagtcg
      421 cgctcacccg cgtcatggcg cgccagctgg gcgagtacgg catcaatgtt aactgcatga
      481 cgccgggctc gaccatgagc gaagaaaatg tttctccaga ggtgctcaaa cggcgcgaag
      541 gaagcatgga caagcgcgct tttcggcggg tcgaaacccc tgccgacatc gtcggcaccg
      601 cgctttttct cgccagctcg gacagcgatt tcgtcaccgg acaactctta gtcgtcgaag
      661 gcggcggcgt catgcactga ctcgcgcttg gaggaatgaa tcgacaatcg cttggtcgcc
      721 gagaagtcgg gtctaattgg aattgcccgt gatgccgtat acatggccgc aggcgttgca
      781 gagcacgacg ttgaacggcg ctaggacaaa atgcttgccg tgcgcgctga cggaacggcc
      841 cttacgcgag actaaatgat cgattcccac caccgcgcat gacggacact tgggctccgg
      901 ttcgattttt tgcttgaagt cttgagagaa cttaggttcg gctctcgatt cgctacggct
      961 cgatgccgat tgttctttat cgctggcctc gtcttcaacc gcgtcgtcgc tctcctcggc
     1021 gccggccgga tgcttgacgc tatcgtaaac tcgccgtttc aaagcatccg agagtaccgc
     1081 ataagcgtca cgcacttgag tgaatttgtc agccgcttcc ggactgtccg gattgcggtc
     1141 tggatgaaag cggaaaacca tctgccggta ggcgcgcttg atgtcgtcca ccgtggcgcc
     1201 gggcttgacc ccgaggatcg aatagtagtc gacggaagta tccaaattgt gcagtccttc
     1261 agtagttggc tagtctgcaa acgccggttt cgaatgcatt aggcttaaag gcgccggccg
     1321 ccatttccgg tcaatcggcc gcgttgctta tacatcccga cacacaaatc gtagatcgcc
     1381 gcaccggtgc gatcgtcgat gacgcgctcg ccgtcacggc tgatatattc tacaatgaat
     1441 gtcgactcct tggtgcgccg gtcgttgttg gataaccgca ccagttttgc ttcgttgcga
     1501 tcgaggaatt cgccgatcgc ccgcagaact tgcggttgat tgagaaaatc cttcggcgcc
     1561 gccaccttgc cgcgctggtt ctcaccggcc cggtccgatt cgtcgatgtc gcttggacga
     1621 tatctcaatt ccacgggagt tgccgccggc ggctcctgat aaccgcacag ggcaacatac
     1681 tcccggcctt cgaaacggat atcaaagctt ttcagtccgc gccggtccaa atcttggccg
     1741 atgctgcgca gcgccgtggc atagtcgatt tttgcgggat tcaccctagt ggcgacccgt
     1801 cgtttggtaa tcatggttct aagaaggatt tattgagtga atggtaagaa cagtagcgca
     1861 aaaaatcaac aaaaaagatc ggcgccgcca tcgttgggct ggcgccgccg atccgaaaag
     1921 ttgcccaata gaactttgtg gaagttgtcg accgttagta agactattct gggagcatgg
     1981 gcagagcgtc catgacgccg atcatgctgt cgcgcgtcac cagtttggcc aaatcggctt
     2041 cgctccaacc ctcggtgccg gcggggcgca acggcataac gatatagcga atatcggcgg
     2101 tgctgtcatg cacccgcact tcgacatcgt cggttagctc caaaccgaac tcgcgcatga
     2161 cgccgcgcgg atccgacacc gagcgctggc gataattgaa gctcttatac caatccggcg
     2221 gccggccaag tatcggccgc ggatagcagg aacatagggt acagaccacc atgtggcgca
     2281 cttgcggcgt gttttccaag acgacgaatt cgttgagcgc ggtggcgtcg atgccgatct
     2341 cggcacaggc cgctttgggg tcggcgatca agcgcgcctt aaactgcgga tcgacccaag
     2401 ctttagcgac gagattggcg ccgttggcgg gagtacgcgc ctcctgatat tcgatctggc
     2461 tctgaatgtc tttctcggtg atgacaccct tctcgaccaa caggtcgcgc atggcgtaga
     2521 tccgtttggc gtggtagctc agttcatgct tgggatgatc atgcccgtgg tcgtcgctca
     2581 taagccgttc tccttaagcc agggtttcga gccagtgatc gtaaatatcg accagcacct
     2641 tgtccttcgc cccctgcgcc ttgccgaaca cttcactgtg gtcgaaggcg acttgataaa
     2701 gcgtcttctt aggcaggccg tccttgccga aggcgagtaa ttcggggttg agaaagtcgc
     2761 cgtagacctt ttcgatccga ccgctcttgc cacggatgta ccagggagtg cgcagatggc
     2821 cgggacgatc ttcgaatttg actttgaccc gatcgccggt cctgaatctg ccggtggcgg
     2881 gagtcacggc tcgccccact tggattcgaa ctcaacgagc tttcggtcga tctccgcctt
     2941 agtgagaata tttttttcga tcaggatcgt ttcgatactg gcgatccagc gctcgtagta
     3001 ggtcatgtcg cggtaggccg cggagtccat ctcctcgcgt gcccggcgca tctcgtcggt
     3061 gcgcttgatg ccgcgcgcgc cgagcgcttg attgactgca tcggcgagaa tctcccaatc
     3121 ggacagctga tgctcggtaa cttcgatggc tcccgcgccc ggaagcccgc cgacatcgtg
     3181 atgaccacgc gccataaaaa atctccccgg ttattttttg agacggttag cgagcgcgcc
     3241 gtgctcttcc ttcatgcccg ccggcagatg atcgccgaag gtatcgaaga aatcgctctg
     3301 gcttttcaaa ttatttttcc aggcctcgcg atccaccgat accaattgat cgacatcggc
     3361 tttggcaata tctaacccat cgccggtcaa gctcgatgcc ttgggcacaa agccgatcgc
     3421 ggtctcgtcg gccgcgccgc cgcctttgga gcgctcgacc acccagcgca ggacgcggag
     3481 attttcgccg aatcccggcc agagaaagcg cccccgctca ttacgctgaa accaattgac
     3541 gcggaaaatt ttcggcgggt tggccgcctt tttacccatc gccaaccagt gcgcccaata
     3601 atcacccatg ttgtagccgc aaaagggcag catcgccatc ggatcgcggc gcaccacgcc
     3661 gacggcgcca gtcgccgccg cggtggtttc ggaagccagg gtcgccccca gaaaagtgcc
     3721 atgctgccaa ttgaag
//
LOCUS       DASQKS010000222         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5151475, whole genome shotgun sequence.
ACCESSION   DASQKS010000222 DASQKS010000000
VERSION     DASQKS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5151475"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VE170_05785"
     CDS             <1..416
                     /locus_tag="VE170_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HYJ14981.1"
                     /translation="GKIHGEYKFDAAATNVTTPVMEFFEKKRGVCQDFTHLMISCLRS
                     TGLAARYVSGYLLTRPAPGKPRRVGADVSHAWIALFVPAVGWVDLDPTNNILPSLEHI
                     TLGWGRDFADVTPLRGVINGGGEPTLEVKVTVEPL"
     gene            complement(490..1218)
                     /locus_tag="VE170_05790"
     CDS             complement(490..1218)
                     /locus_tag="VE170_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ14982.1"
                     /translation="MIESLLLAVTRVSTMSGDNLLTGATGFFFERDGRLFLVTSRHVA
                     IDEASNHRPDRLTIELHVDRQNIADVIQWSIPLYDGDQALWRQCVDSGGTVDVVAVEI
                     HRQALPQTVLFSPFTPNHLVAELDQVEVGTAVLIVGFPLGFHDNLHHLPVARHAGIAS
                     AFGIRFQGQGYFLTDARMHRGTSGAPVVARVLTERSGRGDLTWTLLGVHAARMDVANR
                     DINEDERLNLNCAWYADVLLTLTA"
     gene            complement(1456..>2052)
                     /locus_tag="VE170_05795"
     CDS             complement(1456..>2052)
                     /locus_tag="VE170_05795"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14983.1"
                     /translation="SGSLAMATYVGLDQLGLVPRRDRITLLNTGSTPERMSSLFAGAI
                     DAAFFNPEVEQVVLQQGFPMLFDLGKSNVPYQASGLVTSRKYLRSNPLIVENLAKAVV
                     EGVAFVQNPANKKTVLESIGRNLRLDRPDRLESAYRAIAEVMPRKPCPTLPGIASVLK
                     LMAQHGINPKAAELKPEDIADMSMCKKLEDSGFFARLQ"
BASE COUNT          435 a          610 c          585 g          422 t
ORIGIN      
        1 tgggtaaaat tcacggcgag tataaattcg atgcggcggc gaccaatgtg accacgccgg
       61 tgatggaatt tttcgaaaag aagcgcggcg tctgccagga ctttacccat ttgatgattt
      121 catgcttgcg ttcgaccggg ctcgcggcgc gttacgttag cggctacttg ctgacccgcc
      181 cggcaccggg caagccgcgg cgcgtcggcg ccgacgtttc ccatgcatgg atagctttat
      241 tcgtcccggc cgtcggctgg gtcgacctcg acccgaccaa taatattctg ccgtcgctgg
      301 agcacatcac gctcggctgg ggccgcgact tcgccgatgt cacgccgttg cgcggcgtga
      361 tcaacggcgg cggcgaacca accttggaag ttaaggtgac cgtcgagccc ttgtaaaaag
      421 gccggccatt gcgtcgatcc aggatcgttc cgcttcactt gtcgcgccat cgttgcaacg
      481 ctaaaacgct tatgcggtta gcgtcagcag cacatccgcg taccacgcgc agttgagatt
      541 caagcgctcg tcctcgttga tatcgcgatt ggccacgtcc atgcgcgccg cgtggacgcc
      601 gagcagcgtc caggtgagat cgccgcgacc ggaacgctcg gtgagaacac gggcgaccac
      661 cggcgcgccg ctggtgccgc gatgcatcct ggcgtcggtc agaaaatagc cttgcccctg
      721 aaaccggatg ccgaacgccg aggcgattcc cgcgtggcgc gccaccggca ggtgatgcag
      781 attgtcatga aaccccaatg gaaagccgac gatcagcacg gcggtgccaa cctcgacctg
      841 atccaattca gcgactaagt ggttgggtgt aaacggcgag aaaagaaccg tctgaggcaa
      901 agcctgccgg tgaatctcca cagcgacgac atcgacagta ccgccggaat cgacacattg
      961 gcgccacagc gcttgatcgc cgtcataaag cggaatcgac cattggatca catcggcaat
     1021 attttgccga tccacatgca gctcgatggt aagccggtcc ggccggtgat tgctggcctc
     1081 atctatcgcg acgtgccggc tggtcacgag aaataaccgg ccatcccgtt cgaagaaaaa
     1141 cccggtggcg ccggtgagca ggttgtcacc cgacatcgtc gagacccggg ttaccgctaa
     1201 gagcaaagat tcaatcaagt tgcgctcccg aaaactcaat aggcagaagt gaatattccc
     1261 atcaagggcc ggtaaaacct attccttgta caccgcgtcg atgaagcggc ttttttccaa
     1321 ttcgtcaacc agcgacatat ccaccagcca agagggcggg tttcaaaccc gcccctacga
     1381 ctcccgattc tttttttgcg tcctttgcgt tctttgcagc cattcttctt ttcggttcgt
     1441 ggtgatgcgt aagactcact gcaagcgcgc gaagaagccg ctatcttcta attttttgca
     1501 catactcatg tcagcgatat cttcgggctt gagctccgca gctttggggt tgatgccgtg
     1561 ttgggccatt aactttaata ccgaagcgat gcccggcaga gtcggacagg gcttgcgcgg
     1621 catgacttca gcgatggcac gataggcgct ttctagccga tcgggacggt cgagacgcaa
     1681 attgcgccca atgctttcga gcacggtctt tttattcgcc gggttttgca cgaacgccac
     1741 accctcgaca accgccttgg cgagattttc gacgatcagc gggttgctgc gcaggtattt
     1801 ccgcgaggtc accagacccg atgcctggta cggcacgtta gatttgccca ggtcgaaaag
     1861 catgggaaag ccctgctgga gcacgacctg ttcgacctcg ggattgaaga acgccgcatc
     1921 gatagcgccg gcaaacagcg agctcatgcg ctcgggcgta ctgccggtat tcaacagcgt
     1981 gatacggtcg cgtcgcggca ccaatcccag ttgatccaag ccaacgtagg tggccatcgc
     2041 cagcgagccc ga
//
LOCUS       DASQKS010000223         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3896565, whole genome shotgun sequence.
ACCESSION   DASQKS010000223 DASQKS010000000
VERSION     DASQKS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3896565"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VE170_05800"
     CDS             <1..261
                     /locus_tag="VE170_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14984.1"
                     /translation="FNCMVHLANLIGRLGVTQRLSGFFALIKATNVPTLYVPAECRSV
                     LDVRQRLIKFPEDDTELRLDDRGNFHGASATRIPLRKAHVIF"
     gene            complement(434..>2534)
                     /gene="glgX"
                     /locus_tag="VE170_05805"
     CDS             complement(434..>2534)
                     /gene="glgX"
                     /locus_tag="VE170_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYJ14985.1"
                     /translation="DQVWHAYLPEALPGQLYGYRVHGPYEPSQGHRFNPHKIVLDPYA
                     KSIGRDLRWADEVFAYRMGDPEADLTFDERDSAPYAPLAVVIDPAFTWGDDKPPRTPW
                     HKTIIYELHIKGFTNLHPGVGEPLRGSYAGLASEVVVNHLLNLGVTAVELMPVHQFIN
                     DRHLVDRGKVNYWGYNSLAYFAPECRYSATSIPQDAVQQFKMMVAAFHAAGIEVILDV
                     VYNHTAEGNQLGPTLSLRGIDNAAYYKLTPEDQRFYMDYTGCGNSLNVRHPRVLQLIM
                     DSLRYWVADMHVDGFRFDLASTLARELYEVDRLGAFFDIIHQDPTLSQVKLIAEPWDV
                     GPGGYQVGNFPVLWSEWNGKYRDGVRRFWKGDGGTLSEFATRLSGSSDLYQSDGRKPY
                     ASINFITCHDGFNLQDLVSYNDKHNEANGENNQDGANDNNSWNCGVEGPTDDPAVNAL
                     RWQQKRNLIATLLFSQGVPMLLAGDELSHTQQGNNNTYCQDSELTWLDWQLTDEEQDF
                     LEFVKQMISIRRSQPVFQRRKFFQGRAIFGTDESDITWFEPSGKPMGEEAWSAGFNQC
                     FGVRMPGDLIGEVDDRGQEIKGDSILILMNAFHEPIPFKIPARVKGQRWQRLIDTALP
                     GAARNWFASGKAYDLKGRSMVVFRTVAGDLAAPDHVKASRRGRTLASAAPTPPDGSIG
                     QPEIESSTAARDPVEGT"
BASE COUNT          528 a          723 c          717 g          566 t
ORIGIN      
        1 tttaattgca tggttcacct cgcaaattta attggtcgcc taggagttac gcaaagactt
       61 tccggtttct ttgcgctaat caaagcaacg aatgtaccaa ccctttatgt ccccgcagaa
      121 tgccgaagtg tcttggatgt gaggcaaagg ctgattaaat ttcccgagga tgatacggaa
      181 ctccgcctcg acgaccgagg taattttcac ggtgcgtccg cgactcgcat tccgttaaga
      241 aaagcccacg tgatttttta aagatggggt ggggatgctg tgcgaagaaa tcgcgaagga
      301 tctcgcgatg ggtctagccg tgctaaatct acctggtcga cccctggccg ttggcttcac
      361 aaactgctgg ggaggctgtt gttcgcgcgg caccggaaag tcgagttacc tgcttctccg
      421 gcccgccgcg gctttatgtg ccttcgactg ggtctctcgc cgctgtcgaa ctctctattt
      481 ccggttgccc gatgctgccg tctgggggcg ttggcgccgc gctcgcgagt gtgcggccgc
      541 gacggctcgc cttgacatgg tcaggcgccg ccaaatcacc cgccaccgtg cggaacacga
      601 ccatcgaccg gcctttgaga tcgtaagctt tgccgctggc aaaccaattg cgcgccgcgc
      661 cggggagcgc cgtgtcgatc aagcgttgcc agcgctgtcc cttgacgcgc gccggaatct
      721 tgaagggaat cggttcgtgg aaagcgttca tcagaatcag gatcgaatcg cctttgatct
      781 cctgcccgcg atcatcgact tcaccgatca aatcgcccgg catgcgcacg ccgaagcact
      841 ggttaaagcc ggcgctccat gcctcttcgc ccatcggctt gcccgaaggc tcgaaccagg
      901 tgatgtcgct ctcatcggtg ccgaagattg cccgcccctg aaaaaatttg cgccgttgaa
      961 agaccggttg gctgcggcgg atggaaatca tctgtttgac gaattcgaga aaatcctgtt
     1021 cttcgtcggt cagttgccaa tcgagccagg taagctcgct gtcctgacaa tacgtgttgt
     1081 tgttgccttg ctgtgtgtgg ctcaattcat cgcccgctaa cagcatcggc acaccctgag
     1141 aaaacaagag cgtcgcgatc aggttgcgct tttgctgcca gcgcagcgcg ttcacagcgg
     1201 gatcgtcggt ggggccctca acaccgcagt tccaactgtt gttgtcgttg gcgccgtcct
     1261 gattattttc gccattggcc tcgttgtgtt tatcgttgta gctcaccaga tcttgcaagt
     1321 tgaaaccgtc gtggcaggtg atgaagttga tgcttgcata gggcttacgg ccgtcgcttt
     1381 gataaagatc gctgctgccg ctcaggcgcg tggcaaattc cgacaaggtg ccgccatcac
     1441 ccttccaaaa acgccgcacg ccgtcgcggt acttgccgtt ccattcactc caaaggaccg
     1501 ggaaattacc cacttgatag ccgcctggcc cgacatccca gggttcggcg attaatttga
     1561 cctgggacag ggtcgggtct tggtgaataa tgtcgaaaaa cgcgcccaag cgatcaactt
     1621 cataaagttc ccgcgccaag gtgctggcaa gatcgaaacg gaagccgtcc acgtgcatat
     1681 cggcaaccca atagcgcaga ctgtccatga tcagctgcaa gacccgcgga tggcgcacat
     1741 tgagggaatt gccgcagccg gtatagtcca tgtaaaaacg ctgatcctcg ggcgtgagtt
     1801 tgtaataggc tgcgttgtct atgccccgca gcgacagcgt cgggccaagc tgattgccct
     1861 ctgcggtgtg gttgtagacc acgtcgagaa tgacttcgat gcctgcggca tgaaacgccg
     1921 ccaccatcat tttaaactgc tgcaccgcgt cctggggaat gctggtggcg ctatatcggc
     1981 attcgggcgc aaaataagcc agcgagttat acccccaata attgactttg ccgcgatcga
     2041 ccagatggcg gtcgttgata aattgatgca ccggcatcag ctccacggcg gtgaccccga
     2101 gattgagcaa gtgattgacc accacctccg aggccaaccc ggcataactg ccgcgcagcg
     2161 gttcaccgac tcctggatgg agattggtaa aacctttgat gtgcaactcg taaataatcg
     2221 tcttatgcca gggcgtgcgc ggcggcttgt catcgcccca ggtaaaagcc ggatcgatga
     2281 cgaccgccaa gggagcataa ggcgcgctgt cgcgctcgtc gaatgtcagg tcggcttccg
     2341 ggtcgcccat gcggtaagcg aaaacttcgt ccgcccagcg caggtcccgg ccgatcgatt
     2401 tagcataggg atcgaggacg attttatgcg gattgaaacg atggccttgg cttggctcat
     2461 agggaccgtg cacgcgatag ccatagagct gccccggcaa tgcttccggg agataggcat
     2521 gccacacctg atca
//
LOCUS       DASQKS010000224         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4735699, whole genome shotgun sequence.
ACCESSION   DASQKS010000224 DASQKS010000000
VERSION     DASQKS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4735699"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VE170_05810"
     CDS             <1..1049
                     /locus_tag="VE170_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYJ14986.1"
                     /translation="LQARSANVGATERRALQDRHILRAIEKQRELGFRIFTDGEFRRG
                     NFMSDFNDAVEGIDEGVAVPRTWQTNAGPSSRASMVPGTVVGKIKQTRRLTEHEFAFL
                     KQQRPGDIKVTLPTANQFPAIYFKKGISDKVYTDHSALLWDIVPIIKAEIHALASAGA
                     RYIQIDAPRYSYYIDPKWRSYVSNEMGLDPDQALDEAIRADNACLEGARRDGVILAIH
                     LCRGNNRSQWYAEGGYDAIAEKLFGRLQVDAFLLEYESERAGTFEPLRFVPRGKTVVL
                     GLVSSKLPAMESADHLARRIDEAAKYVPLENLAISPQCGFASTMEGNLLTEEQQWQKL
                     KLVVDSAIKVWGQT"
     gene            1152..1796
                     /locus_tag="VE170_05815"
     CDS             1152..1796
                     /locus_tag="VE170_05815"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14987.1"
                     /translation="MPANVEVLYEQLVLHDGVLNDVEGKLDLIVGRAVQFADKQNLDG
                     LIFPGAPREVLNPNLSDAFTAALKIPVATALRASAAALTTLAAKRILLLTPFDPSLNR
                     LICDFLGWFDLIAISPGETLPHYTDALKMSSDDVIAYARRAIIDQREVDAIYFQGAVL
                     DPTDCLEQMERELNLPVVASNPALHWYMLSKLGLKHPLHGFGKLLASWPALPFS"
     gene            2061..3008
                     /locus_tag="VE170_05820"
     CDS             2061..3008
                     /locus_tag="VE170_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ14988.1"
                     /translation="MTFVVNMAAGDANDLWARALARSLVKYIPGNPNIVAQNMAGGGS
                     LISANYLYSVAKPDGLTIGQTSGSLYFQQLIGRHEVQFDWRKFSWIGSAGLVEALLMM
                     RADAPYKSMEDIRTAGEPPKCSATAPGSSGHISLKVLEDALGARFRIVTGYKSGSDQD
                     LAIERGEVQCRSVSSASFLAREPFISWQKKGFIRILVQSGRKRNPKLAEVPTLYELMT
                     RHQTPEAKQRLAAVLLGADHFGQYLAVAPPGTPAGRVEILREAYTRALKDADLIEEAR
                     KRNWSMEQVSGAELQALAQEVIDQPPQLIGRVKELLGGT"
     gene            3128..3418
                     /locus_tag="VE170_05825"
     CDS             3128..3418
                     /locus_tag="VE170_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYJ14989.1"
                     /translation="MIQEIVIQQVQPEKRDEYIRVFGDILKKANYAGSHGIKFFTSVE
                     DPSRVILMIEWDSVEAHTQHRGTPVHNAMREATSKYQTVKSDGAHFVMHVVK"
BASE COUNT          777 a          977 c          998 g          690 t
ORIGIN      
        1 tattgcaggc acgcagcgca aatgtcggcg ccacagagcg gcgcgcgctt caagaccggc
       61 atatcctgcg agcgatcgag aagcagcggg aactcggctt caggatattc accgacggcg
      121 agtttcgccg cggcaatttc atgagcgatt tcaacgatgc cgtggaaggc atcgatgaag
      181 gggtggcggt gccgcgcact tggcaaacca acgctggtcc atcgtcccgc gcgtcgatgg
      241 tgcccggcac cgttgtcggc aagatcaaac aaacccggcg cctgaccgag catgagttcg
      301 ccttcttgaa gcagcagcgc cctggcgaca tcaaagtcac cctgccgacc gccaatcaat
      361 ttccggcgat ttatttcaag aaaggcatca gtgataaggt ctacaccgac cattccgcgc
      421 tgttgtggga catcgtgccg atcatcaagg cggagattca cgccttggcc agcgcaggcg
      481 cgcgttacat ccaaatcgac gcgccgcgct acagctacta tatcgatccg aagtggcgca
      541 gctacgtgag caatgaaatg gggctcgatc ccgatcaggc cttggacgaa gcgattcgcg
      601 ccgacaacgc gtgcctcgag ggagcgcggc gcgacggcgt gatcttggcg attcatctgt
      661 gccgcggcaa caatcgcagc caatggtacg ccgagggcgg ctatgacgcc atcgccgaaa
      721 aactgttcgg ccggttgcag gtcgacgcgt ttttacttga gtacgaatcc gaacgcgccg
      781 gcactttcga gccgctgcgc ttcgtgccgc gcggcaagac cgtggtgctc ggcctggtca
      841 gcagcaagct gccggcgatg gagtcggcgg accatctcgc caggcgcatc gacgaagccg
      901 ccaagtacgt gccgctggag aatctcgcga tcagtccgca atgcggcttt gcctcgacga
      961 tggaaggcaa cctgttgacg gaagagcaac aatggcaaaa actaaaactg gtggtcgata
     1021 gcgcgattaa agtttgggga cagacgtaaa tcactaacca accgccgatg ccagataatc
     1081 gacaaacgag aataggttgg ctcgggccga gtctaccgtc cagctcacat ataaaaaagt
     1141 ttctcgacct gatgccggcc aacgtcgaag tgctttacga acagctggtg ctgcacgatg
     1201 gggttttgaa tgacgtcgag ggcaagctgg acttgatcgt cggccgcgcc gtgcagttcg
     1261 ccgataagca aaacctagac ggcttgatat tccccggcgc gccgcgcgaa gtgctaaatc
     1321 ctaatttgtc cgacgccttt accgcggcgc tcaagattcc tgtcgccacg gcgttgcgcg
     1381 cctcggcggc ggcgctgaca actctcgcgg ccaaacggat tcttttgcta acgccgtttg
     1441 acccatcgtt gaaccggctg atctgcgact ttctcggctg gttcgacctg attgcgattt
     1501 cgccgggcga aactttgccg cactacaccg atgccctcaa gatgagctct gacgacgtca
     1561 tcgcctacgc gcgccgggcg atcatcgacc agcgcgaagt cgacgcgatc tattttcaag
     1621 gcgcggtgct cgatccgacg gactgtctcg aacagatgga gcgcgaattg aatctgccgg
     1681 tggtcgcgag caatccggcg ctgcattggt acatgctgtc caagcttgga ttgaaacacc
     1741 cgctgcacgg attcggcaag ctgttggcca gctggcccgc gttacctttt agctaaaacg
     1801 acctctaccg cgaacgtata atgagcagct cgatctatgc ttggcagttc ccccgtttga
     1861 aaaagggggg taagggagga ttttctataa gaggattttc acaggcggaa ctttcccacg
     1921 cacgcaaaaa caaatccccc ttagtccccc ttttccaaag ggggatggga ctgctcgctt
     1981 cgcttctcag cttcgccgca atggtgcttt tcgcgaacat ccccgcatcg gctcaaagca
     2041 atttctatcg aggcaaaact atgacgttcg tcgtcaacat ggcggccggc gatgccaacg
     2101 atctttgggc acgcgctttg gcgcgcagtt tggttaaata tattccaggt aaccctaata
     2161 tcgtcgccca aaacatggcg ggcggcggtt cgctgatctc ggccaattat ctttacagcg
     2221 tcgccaaacc ggacggcctc accatcggtc aaacctcggg cagtctttat tttcagcagc
     2281 tcatcggtcg gcacgaagtg cagttcgatt ggcgcaagtt cagctggatc ggctcagcgg
     2341 gactggttga agcgctgctg atgatgcgcg ccgatgcgcc gtacaaatcg atggaagata
     2401 ttcgcactgc cggcgagccg cccaaatgtt ccgccacggc tccggggtcg agcggacata
     2461 tcagcctcaa agttttggaa gacgcgctcg gcgcgaggtt tcgtatcgtc accggttaca
     2521 agagcggcag cgatcaagac ctcgccatcg agagaggcga agtgcaatgt cgttcggtgt
     2581 cctcggcgtc gttcctcgcc cgcgaaccgt ttatcagttg gcagaaaaaa ggctttatcc
     2641 gtatcctggt acagagcggg cgcaagcgaa atcctaaact cgccgaagtg ccaacgcttt
     2701 acgaactgat gacgcgccat caaacgcccg aagccaagca gcgtctagcg gcggtgctgc
     2761 tcggcgccga tcatttcggc caatacctcg cggtcgcgcc gccgggaacg cccgccgggc
     2821 gcgtggagat tctgcgcgaa gcctacaccc gcgcgttgaa agacgccgat ctgatcgaag
     2881 aagcgagaaa acgtaattgg agcatggaac aagtcagcgg cgcagaactg caggctctgg
     2941 cacaggaagt catcgatcag ccgccgcaac tgatcggacg agttaaagaa cttttgggcg
     3001 gtacctagta tgtttgccgc gcagttgtgc gttgacacaa ggctggcctg ggcagcttca
     3061 ggaatggcgc ggtactgaac gttttgagag gtttaaacga tttgaaccat ttgaacagga
     3121 gaataacatg atccaagaaa tcgtcattca acaggtgcaa ccggaaaagc gcgatgagta
     3181 tatccgggtt tttggcgaca ttctcaagaa agccaactac gccggttccc atggcatcaa
     3241 gttttttacc agcgtcgaag atccgagccg ggtgatcttg atgatcgagt gggacagcgt
     3301 cgaggcgcat acccagcatc gcggcacgcc ggtgcacaac gccatgcgcg aggcgacgtc
     3361 gaagtatcag acggtaaaaa gtgacggcgc gcactttgtc atgcacgtgg tcaagtgaag
     3421 cagcgcattc atggctcaga tt
//
LOCUS       DASQKS010000225         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3057751, whole genome shotgun sequence.
ACCESSION   DASQKS010000225 DASQKS010000000
VERSION     DASQKS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057751"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VE170_05830"
     CDS             complement(<1..973)
                     /locus_tag="VE170_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14990.1"
                     /translation="MKRIADRLPMFALVAMLVISTAQAAERPYYQGKTVTFFINFSAG
                     GPTDIEGRIVARHLARHVPGNPTIVVQNMSGGGGVTGINFLGERAKHDGLTLGYFTGP
                     YNHQMMKSSTLRVDLMKVPFIAAVAGVTVCYIRSDVAPGIKKPTDIAKAERFRAGGLS
                     FDSNKDLRFRLAFDVLGVKYDYVTGYNSSNDARLAVQRNEIQYHDENIPGYRGVVEPQ
                     MVKTGMVTPLYYHDVIKPDGTMAKSPDYPELNSFTEVYTQINGKAPSGIKYEALKAAN
                     MASQNLNRVALLPPNSPNESVLAMRQAFTQLAKDEEFISEAKKVMRFQ"
     gene            complement(992..1969)
                     /locus_tag="VE170_05835"
     CDS             complement(992..1969)
                     /locus_tag="VE170_05835"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ14991.1"
                     /translation="MLKSFSMAVILVLILPWVGAAQSKVKFPYSITSKSLGYGPMLAS
                     AKLGFMEREELEPQLVYVRGADKSLAAVLSGSVFVSASGADAHIAAVERGVDLSMIGG
                     TINGLSQVLMTTKQFPTLNDLRGKVIGATSATSGLGFALRRYFKANGFEFGRDYQILA
                     TGSAGPTLAALAAGQVQGAALGVPLNFIAADQGFHGVGRIVDVIPSYQFGAFTVSRPL
                     AEKNRPLMVRFMRAMIAVHRWFYASKEAAVAFMAKELDMTPDYARRGWEYYVEHKIWP
                     IDASVNIDGMNMATQIYWEASRSKGAVPSGSKYIDHSFQKDALKELAGR"
     gene            2533..>2746
                     /locus_tag="VE170_05840"
     CDS             2533..>2746
                     /locus_tag="VE170_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ14992.1"
                     /translation="MARIKFGVGLYGTDSAQEAVQLARTAEALGYDRFWIGDSHMIWR
                     ELYVLAGAIAMMTQKIAIGPGVTHPAV"
BASE COUNT          593 a          812 c          716 g          625 t
ORIGIN      
        1 gctggaagcg catgaccttt tttgcttcag agatgaactc ttcgtccttg gcgagctggg
       61 taaaagcctg gcgcatcgcc agcaccgact cattgggaga attgggcggc aatagggcaa
      121 cgcggtttag attctggctc gccatgttgg cggccttgag cgcctcgtac ttgataccgc
      181 tcggggcctt gccattgatc tgcgtataca cctcggtgaa tgaattgagc tcaggatagt
      241 ccgggctctt cgccatcgtc ccatcgggtt ttatgacatc gtgataataa agcggcgtga
      301 ccatgccggt cttgaccatc tgcggctcaa ccacgcctcg gtaccccgga atattttcgt
      361 cgtggtactg aatctcgttg cgctgcaccg cgagccttgc atcgttgctg ctattgtagc
      421 cggtcacgta atcatacttg acgccaagca cgtcgaaagc caggcgaaaa cgcaggtcct
      481 tgttgctgtc gaatgacaga ccgccggcgc gaaaccgctc ggccttggca atgtcggtcg
      541 gtttcttgat acccggagcg acatcggagc gaatgtaaca gacagtgacg cccgccaccg
      601 ccgcgataaa cggcactttc atcagatcga cgcgcagagt cgagcttttc atcatttgat
      661 ggttataggg gccggtgaag tatcccaggg tcaaaccatc gtgcttggcc cgttcgccga
      721 ggaaattgat accggtgacg ccgccgccgc cggacatgtt ctgcacgacg atggttggat
      781 tccccggtac atgacgcgcc agatggcgcg caactatgcg tccttcgata tcggtcggac
      841 cgccggcact gaaattgata aagaaggtaa cggtcttgcc ctggtaatag ggccgctccg
      901 ccgcctgggc cgtcgatatt accagcatcg cgaccagcgc gaacatcggc aaacgatctg
      961 caatccgttt catttagctt cctccttcag gtcaacgccc ggccagttcc tttagcgcgt
     1021 ctttttgaaa actatggtcg atatatttgc tgccgctcgg caccgctccc tttgaccgcg
     1081 acgcttccca ataaatttgt gtcgccatgt tcatgccgtc gatattcacg ctggcgtcga
     1141 tgggccaaat tttatgctcg acgtaatact cccaaccgcg ccgcgcataa tccggtgtca
     1201 tgtccaactc cttcgccata aacgccaccg ccgcttcctt gctagcataa aaccagcggt
     1261 gcaccgcaat catggcgcgc atgaagcgca ccatcaacgg ccgattcttc tccgccaggg
     1321 ggcgggaaac cgtaaacgcg ccgaattggt agctcgggat aacgtcgacg atccggccga
     1381 cgccgtgaaa gccctgatca gccgcgataa aattcaacgg cacccccagc gccgcccctt
     1441 gcacctgtcc cgccgccagc gcagccaacg tcggcccggc gctgcccgtg gcgagaatct
     1501 gatagtcgcg gccgaattca aagccgttgg ctttgaaata acggcgcagt gcgaagccca
     1561 agccggaggt cgcgctggtg gcaccgatga ccttaccgcg caagtcattg agcgttggaa
     1621 attgtttggt cgtcatcagg acctgcgaca agccgttgat ggtgccgccg atcatcgaca
     1681 gatcgacgcc gcgctccact gcggcgatat gggcatccgc accggaagcc gagacgaaga
     1741 ccgaaccgct aagcaccgcc gcgagcgact tatccgcccc gcgcacgtaa accaactgcg
     1801 gctccaactc ctcgcgctcc atgaagccta acttagccga cgccaacatc ggcccataac
     1861 cgagactttt gctggtgatg ctgtatggga acttgacctt ggattgagct gcgccaaccc
     1921 atggtaaaat caacaccaat atcaccgcca tcgagaagct tttcagcata ttttccaacc
     1981 ctgttggaat gatcgaggca tgaagtgcca ccaagttttg tattgaggag tacatcggga
     2041 acggttttgc cgtcaagcta ccttggaaat ggctagggat tcagtctaaa tttttcaatt
     2101 agcaggctcg catctctggg tctaagggca gcttctttag aatctcttcg tctatcggag
     2161 tcaacgccca acacttcgga caaataataa cgcctccatc ttctactttt ttcgtccgta
     2221 tcgtttcggc gattttcact gtttgtgtcg ccgaaatgtc ggctacgacg gtgaggattt
     2281 ccctgcacaa gacatatcca atgactggtt ttgccatagt tgccgtcaat ctatatcaat
     2341 tgcaattatc acggcaaatt gacgacacct ggttgctccc caaccttagt tgcgatgaaa
     2401 agcgatagtg ccggctgatt gaccgtatct ggcaccccaa agtcaatatt tttcccggcc
     2461 ttcgtggcct tcgcaccttt cgtagtgcaa gataatctca ctgctaattt tgtattcagc
     2521 gcgaggacac ctatggctcg aataaaattt ggcgttgggc tctacggcac cgatagcgcg
     2581 caggaagcgg tgcagctggc acgcacagcc gaagcgctcg gctacgaccg attctggatt
     2641 ggcgattcgc acatgatctg gcgcgagctc tatgtcctcg ccggcgccat cgccatgatg
     2701 acgcagaaaa tcgccatcgg tcccggcgtc acccatccgg cggtgc
//
LOCUS       DASQKS010000226        10622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_115752, whole genome shotgun sequence.
ACCESSION   DASQKS010000226 DASQKS010000000
VERSION     DASQKS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115752"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..818)
                     /locus_tag="VE170_05845"
     CDS             complement(258..818)
                     /locus_tag="VE170_05845"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HYJ14993.1"
                     /translation="MARLGKILIALLLLAGVSSPLEAAEREKGILEVRIKDHRDAIGD
                     FDKLVITIDKISVSPKPGLKIWQTGWKDLVITSEPIDLTKYVGKKTAQVFRAPIEVGT
                     FDAFHLKIKNAAGVLKKNQRKAIVKNTIGPFKLAYEVQPKTETLLILDLVVSDMSDHP
                     PRGYELSLKGYELFINGKLIEKIPPG"
     gene            complement(794..1414)
                     /locus_tag="VE170_05850"
     CDS             complement(794..1414)
                     /locus_tag="VE170_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ14994.1"
                     /translation="MTSKSKSKLGLGAVLLALGFLTTEVKAHIPLPPKKGEIGRRETN
                     VAAPSFTLTNQAGKQFRFHAASGTFVLATFVYTTCPDVCPLFSAHFAAIQRALEAEKQ
                     QNYLLLSISTDPKRDTPAKMKSYAEAFKADFKHWQFLTGSQKDLSEVWKNFGVNVKDH
                     GNGQIQHTNLTTLIDAKGHRRVDYYGDKWLVKEVLKDIKWLASEKS"
     gene            complement(1392..2279)
                     /locus_tag="VE170_05855"
     CDS             complement(1392..2279)
                     /locus_tag="VE170_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYJ14995.1"
                     /translation="MNMSNLLWSAAAIVMMALPPYFLLSAPDNGGPSGAVRTINSYLK
                     SAYAHDHKKAYRLISNRDRQIKSEDLYVQEKGPFSGFTLKVAGKLAEMIEVRPTGATE
                     SGATTKVKFDIKLPDASSLSALLLDWDQDKLNTLPEPKQKEILAAIDNLRRAGKLNFI
                     EGEEELTLVKENGRWRVWFDWATGLRVHYDATVPNNDVIEATPLNRETSVRANELFTI
                     AYRVRNTSQETIATRIAHRVEPEELREHLDLVECALLLPVKLKPGEDREFSSTYMVRG
                     DLPDGSKELKVTYDFKVEK"
     gene            complement(2276..3190)
                     /locus_tag="VE170_05860"
     CDS             complement(2276..3190)
                     /locus_tag="VE170_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14996.1"
                     /translation="MELRAISRRRLCATILLAAVLTLAPLASPTRADDRAEIITVLNR
                     YVRALYARDFRAVYEQLSSADQRLKDVHSYSRERGEFRDFTLEVAQAVARSVEVNLIA
                     QSVEAARATVKVKVNVPDASKLTASLLDWDSERLERLSATERKTLLETIDRQKREKSI
                     AMVEGEETFNLVKEPGGWKIFLDWAAGVKLIFQPAIPASASVEITIEQKEVASRPGQI
                     FRVAMKIKNTGKQTLSVRIGHLIEPQELRDYLDLVDCGFILPLRLPHGKEEEFVTTYL
                     LRGTLPEQVRQLHVTYAVNASPLESAKP"
     gene            complement(3235..3822)
                     /locus_tag="VE170_05865"
     CDS             complement(3235..3822)
                     /locus_tag="VE170_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ14997.1"
                     /translation="MNRRMFYQARTGLGLLVLSTFAVIAQDSQYAIAVAQDTIFENKK
                     IVPFVPTPQEVVDKMVDLAGIKKGDVVYDLGSGDGRIVVTAAKKGAKAVGFEIDGDLV
                     KQSRDNIRKAGVQDQAEIRQQDILTVDLSAASVVTMYLLPDVNLQLRPNILSQLKPGS
                     RVVSHAFDMGDWKPDRIERVEGRTLYLWTIPAKAR"
     gene            complement(3904..4785)
                     /locus_tag="VE170_05870"
     CDS             complement(3904..4785)
                     /locus_tag="VE170_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ14998.1"
                     /translation="MHRAKLIFTALLIALLAVVAYLRIRNTNIEADLPRATLDAYLRA
                     TYARNFTVAYDYLSSADRQVRTRQNYVDSQGGYSGFTLDVARQLAGFMEIWSIDRHES
                     GGRLIMKVGYRVPAPAELNELLLHWDEARLNSLSTKMQKDIVAKLTARNKSANLLNIE
                     GQETVELIEEPEGWKVHLDWAAGTRVVLASRLSDSHRLDVRFASTEVIAKSDELFLVN
                     VRIKNPTAHAVSFSVNHLLEPPAIADDLQLVDCGLLTPVTLAPQQEKEFAMAYLLDRP
                     AGKSQRRVKLTYDFKIE"
     gene            complement(4908..5978)
                     /locus_tag="VE170_05875"
     CDS             complement(4908..5978)
                     /locus_tag="VE170_05875"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HYJ14999.1"
                     /translation="MGALVIQGAKLQHGELPSLEILWRYLTATQSGKVWLIRAGYAIA
                     LALLGFSITRGTARLHGIRWLALLSLPLIASRSLTSHAIAVKLHTSWVIAADAVHLIT
                     TALWAGGLVAVLTTVRAKRNQADLSLVWLAQFINRFSRLAFVSVALFFCAGLYLSWVH
                     VGSLTTLVATDSGNVLIVKLGLFAAMLTLGAINFLSTRPAIRDMAHADVGNNSLVDKA
                     TTRIVVESMLGLAIFATTGYLTVLPPGVHALHAARQNFPPAQASALQPAQGAKVEIIA
                     PVNNQAVTGDRVELRFNLTKGNRGHHAHAYIDGELIGMFEGNGGTLNGIKPGRHMLEV
                     RVVADDHQTELDATDKVLFIAK"
     gene            complement(6158..6550)
                     /locus_tag="VE170_05880"
     CDS             complement(6158..6550)
                     /locus_tag="VE170_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HYJ15000.1"
                     /translation="MSRPMFFRCLIGLSFFLWAFVIVTATPPVWAHAYPAVSVPNNGA
                     TLKEAPNEVRIQFTEAIEMAFSAVTVIGPNKEIVSQGKLRKLTDDSLAIGLKPLQAGN
                     YTVEWQVLSVDTHVTEGVLRFTIVPSGK"
     gene            complement(6510..7286)
                     /locus_tag="VE170_05885"
     CDS             complement(6510..7286)
                     /locus_tag="VE170_05885"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HYJ15001.1"
                     /translation="MGFQSPLVAHDGWVEIAPVIVERNQPATIALIQGNHSNEHKSYR
                     IAGKWNAQYVTLAVVEANGKQTSLTDRLFDMGEDEEKVGPKGPKGFLLAPFTPQADGL
                     FHAYARQVRLVQQGDGPKVVTVRVAKTPFAAFKIPTIAAARNLKGFDRAIGGDDVAEF
                     IPLNNPLAVYSGGSITLELRQRGKPIGGQIVTLVRRVDGFASVLERTTDDNGRVQFPV
                     GPADSYLARVKIDEESARPDGQKDKTSHESTYVFPVFNRP"
     gene            7530..7754
                     /locus_tag="VE170_05890"
     CDS             7530..7754
                     /locus_tag="VE170_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15002.1"
                     /translation="MVQAKSNLSDKPQVATCCHGLKYTGRFVDWLIVMVRLIVLIAVV
                     FVGFCRLGERVMHAMGRAVSEKKKQDASYE"
     gene            complement(8063..8491)
                     /locus_tag="VE170_05895"
     CDS             complement(8063..8491)
                     /locus_tag="VE170_05895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ15003.1"
                     /translation="MGAGYGALSKYLLERFPNATAICQDGSEEMAKLGRERMKPLAGR
                     FEYVLCDFGKHGWSKLVPGPFEAIVSSIAIHNVGSSRIIRGIYEDAYTLVKPGGCFLN
                     FDRSSPPWQEQMHWLRGAGFKDVQIFWQDENRAVFGGFRN"
     gene            complement(8494..8700)
                     /locus_tag="VE170_05900"
     CDS             complement(8494..8700)
                     /locus_tag="VE170_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15004.1"
                     /translation="MTDSTHSSGHHRHGDPEHAHDHDPHDWTSPEYVSKWAQGQDPKE
                     ANRAAAFSRLADTIPFEKPPRSIS"
     gene            complement(9007..9834)
                     /locus_tag="VE170_05905"
     CDS             complement(9007..9834)
                     /locus_tag="VE170_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ15005.1"
                     /translation="MRNDTLGVPLIASLIVHLLVMLLGSVIFHHTAPRHWDLLPIDLV
                     DAPRPETAPLPQKAEAKKAIKKPSPSAPPKPEKPKSAPALAKAPVVNPEAPLPITKED
                     PAKAIEVKPLAPAKVEPPPSYASGARSEGGGSEAGPGNLFGKGDVGVVPGAGTAGGGG
                     GTAPFGLGRGGSGAPGLPAQEPIFRTNREAKPIQTVRAAYPPMALRAGMESDVTLKIE
                     VDPQGSVTRAEITRSGGAGFDEEALKAVKQSRFEPAQRDGKNVPAEFIYIYRFRLSR"
     gene            complement(9824..10165)
                     /locus_tag="VE170_05910"
     CDS             complement(9824..10165)
                     /locus_tag="VE170_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ15006.1"
                     /translation="MLVFFMISTLSMACYSGLPVNLPKVATGRQTPSQSAALTIASDD
                     TIAMQDVPCAQVGEILKARLNKSPDLLIIINADERVDHGLVVEIMDQARQAGVAKMAI
                     AIKPKESRSEK"
     gene            complement(10433..>10622)
                     /locus_tag="VE170_05915"
     CDS             complement(10433..>10622)
                     /locus_tag="VE170_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15007.1"
                     /translation="YCIGFIMSPFWTLLAGWLMSTSGFQTTFGVISCSYFLGMTLLLF
                     FRDPVKRLRPLEAEPAHS"
BASE COUNT         2268 a         2843 c         3022 g         2489 t
ORIGIN      
        1 ttcccgacaa cggattttat cggttaattg ggtcattgtg tagagcggcg aggattttgc
       61 cgatggtcgc ggcgccgtag ccgcggcaca cgggcgccgc acaacatcgc cgcgattgcc
      121 ttgacagtct cgttctcggc aacttaccaa acaacggtta cgagctcata ggccagggcg
      181 caagccgtcg gtcgagccag taggtgatta gagtcgccgg cgtcggaatt ggatgattgt
      241 ttcgtgtgca atcgccatca gcccggcgga attttctcga ttaatttccc gttgataaac
      301 agttcatagc ctttcaaact caattcataa ccgcgtgggg ggtggtcgga catgtcggag
      361 acgactaaat ccaggattag gagggtctcg gttttcggct ggacttcgta ggcgagtttg
      421 aacggaccga tggtgttttt gactatggct ttgcgttggt ttttctttag tacaccggcg
      481 gcattcttta ttttcaaatg aaaagcgtcg aaagtgccga cttctatggg cgcgcggaac
      541 acttgggcgg ttttcttgcc gacatacttg gtcagatcga taggttcgga agttataacc
      601 aaatctttcc agccggtctg ccaaatcttc agtcccggct ttggacttac ggagattttg
      661 tcgattgtga taaccagctt gtcgaagtcc ccgatggcgt cgcggtggtc tttgatgcgg
      721 acttctaaaa tccctttttc tcgctcagcg gcttctagcg gactcgacac gcccgccaac
      781 aacagcaagg cgattaggat ttttccgagg cgagccattt aatgtctttc agaacttctt
      841 tgacgagcca cttatcgccg tagtagtcga cgcgccggtg gcccttggcg tcgatcaacg
      901 tggttaggtt ggtatgctgg atctggccgt tgccgtggtc tttgacgttg acgccgaagt
      961 ttttccagac ctcggacaaa tctttttgcg agccggtgag aaactgccaa tgcttgaaat
     1021 cggccttgaa cgcctcggca tacgatttca tcttagctgg cgtgtcgcgt ttgggatcgg
     1081 tgctgatgct gagcagtaag tagttctgct gcttttctgc ttcgagggca cgctgaatcg
     1141 ccgcaaagtg agcgctgaac agcgggcaaa catccgggca ggtggtgtag acaaaggtgg
     1201 ccaggacgaa tgtgccactc gccgcatgga agcgaaactg tttgccggct tgattggtga
     1261 gggtgaagct cggtgccgcg acgttagttt cgcgccggcc gatctcgcct ttcttcggcg
     1321 ggagtgggat atgagcctta acctcggtag tcaaaaagcc cagcgcaagt agtactgcgc
     1381 caaggccgag cttacttttc gactttgaag tcataggtga ctttgagctc tttggaacca
     1441 tcgggcaggt cgcctcggac catgtacgtc gaagaaaatt cacggtcctc accgggtttc
     1501 agcttgaccg gtagcagcaa agcgcattcg accagatcga gatgttcacg cagctcttcg
     1561 ggctcgacac ggtgggcgat acgggtggcg atggtttctt gagaagtgtt gcgcacgcgg
     1621 taagcaatcg taaagagttc gttggcgcgc acgctggtct ctcgattgag cggcgtcgct
     1681 tcgatgacgt cattattggg cacggtggcg tcgtagtgca cgcgcaggcc ggtggcccag
     1741 tcgaaccaaa cgcgccaccg gccgttttcc ttgaccagag tcagttcttc ttcgccttcg
     1801 atgaagttca gtttgccggc gcggcgcaga ttatcgatgg cagcgagaat ctctttctgt
     1861 tttggttcag gcagcgtgtt gagtttgtct tgatcccaat cgagtaacag tgccgacaga
     1921 ctgctagcgt cgggcagctt gatgtcgaat ttgactttgg ttgtcgcgcc gctttccgtt
     1981 gcgccagtcg gacgcacttc gatcatctcg gcgagctttc cagctacctt caaggtgaaa
     2041 ccgctgaacg gtcccttctc ttggacatag agatcctcag acttgatttg ccggtcgcga
     2101 ttcgagatca gccgataggc ttttttatga tcgtgggcgt aggcgctttt cagataactg
     2161 ttgatggtac gcaccgcccc ggacggaccg ccgttgtcag gcgcggagag tagaaaatac
     2221 ggcggcagcg ccatcattac gattgccgcg gcggaccaaa gcagattgga catgttcatg
     2281 gtttagccga ttcgagcggt gacgcgttca ccgcataggt gacatgtagc tgccgcactt
     2341 gttcaggcag ggtgccacgc aatagatacg tcgtgacgaa ctcttcttcc ttgccgtgcg
     2401 gcaagcgcaa cggcaggata aagccgcagt cgaccagatc gagatagtcg cgcaattctt
     2461 gcggctcgat cagatggcca atgcgtactg agagagtttg tttgccggtg tttttgattt
     2521 tcatggcgac gcgaaatatt tgtccgggcc gagacgcaac ttctttctgc tcaatcgtga
     2581 tttccaccga cgccgatgcc ggaatcgccg gctgaaagat cagcttaacg ccggccgccc
     2641 agtcgagaaa tatcttccag ccgccgggtt ctttgaccag attgaatgtt tcctcgcctt
     2701 cgaccatcgc gatgcttttt tcgcgctttt gccggtcgat ggtttccaac agcgtcttgc
     2761 gctcggttgc ggataagcgc tcgaggcgct cgctgtccca atcgagcagg gatgcggtta
     2821 atttcgaggc gtccggcaca ttgactttga ctttcaccgt ggcgcgcgcg gcctcgacgc
     2881 tttgcgctat caaattcact tccacgctcc gcgccacggc ttgtgccact tcgagggtga
     2941 aatcgcgaaa ctcaccgcgt tcccggctgt aactatgcac atcctttaga cgctggtcag
     3001 cgctggataa ttgctcgtag acggcgcgaa agtcgcgggc gtaaagcgcc cgcacatatc
     3061 tattgaggac tgtgatgatt tccgcgcgat catcggccct ggtcggcgac gccaaaggcg
     3121 cgagagtcaa gacggcggca agcaaaatcg tggcgcaaag ccggcgccgc gaaatagccc
     3181 gtaattccat caaattgcca ggaaagcagc ggttgatgca atcaatccga gcgatcagcg
     3241 agccttggct ggaatcgtcc atagataaag ggttcggccc tcgactcgct cgatgcgatc
     3301 aggcttccaa tcgcccatgt cgaaggcgtg ggagacaact cgggagccgg gtttgagctg
     3361 gctcaggata ttcggtcgca attgcaaatt aacatccggc agcaaataca tcgtgactac
     3421 cgacgcggcg gaaagatcga cggtgagaat gtcttgctgg cgaatctcgg cctggtcttg
     3481 aacgccggct ttgcgaatgt tgtcgcgcga ttgtttgacc aagtcaccgt cgatctcaaa
     3541 gcccaccgct ttggcgcctt ttttcgcggc ggtgacgacg atgcggccgt cgcctgaacc
     3601 gagatcgtag acgacatcgc cttttttgat gccggcgaga tcgaccattt tatcgacgac
     3661 ttcttgcggc gtgggcacga agggcacgat ctttttgttt tcgaagatgg tgtcctgggc
     3721 aaccgcgatg gcgtattgcg agtcctgcgc gatgacggca aaagtcgaca gaaccagcaa
     3781 tcccaagccg gtgcgcgctt ggtaaaacat tcgacggttc atgggaattc atctcctctt
     3841 taaagattgg ctgtcagcaa ttaaccataa aatatttacc gtagccagta tgacctcgcc
     3901 aggttattcg atcttgaaat cgtaagttag tttgactcga cgttggctct tgcccgccgg
     3961 gcgatcgagc aaatacgcca tggcgaattc tttttcttgt tgcggcgcga gtgtcacggg
     4021 ggtcagcagg ccgcaatcga caagctgcag atcgtcggcg atggccggcg gctccaataa
     4081 gtggttgacg gaaaaactca cggcgtgagc ggtcggattc ttgatcctga cgttgaccag
     4141 aaacaactcg tcgcttttgg cgatcacttc agtgctggcg aagcgaacgt ccagcctatg
     4201 actgtcggat aatcggcttg ccagcactac gcgcgtgccc gccgcccagt cgaggtggac
     4261 cttccagcct tccggttctt cgatgagttc gacggtttct tggccctcga tattgagcaa
     4321 attggccgat ttattgcggg cggttagctt ggcaacgata tctttctgca tcttggtgga
     4381 gagcgagttc aaacgcgctt cgtcccagtg caacagcaac tcattgagtt ccgccggcgc
     4441 cggaacccgg taaccgactt tcatgatcag gcgtccgccg ctctcgtgcc gatcgattga
     4501 ccagatttcc atgaagccgg caagttgccg ggccacatcg agggtaaagc cgctgtagcc
     4561 accttggcta tcgacataat tctggcgtgt gcgcacctgc ctgtccgcag acgaaagata
     4621 gtcgtatgcc acggtgaagt tacgcgcgta ggtcgcgcgc agataggcgt ctagagttgc
     4681 gcgaggcagg tccgcttcta tattggtatt ccgaattctt aagtaagcga caacggccaa
     4741 tagagcgatc agtaaggcgg tgaatatgag tttggcgcga tgcatcgcta gttactcgag
     4801 cagcgggagt ttttatcgtt ccatcggttt gacgaatcaa agcgcattaa ccgttgaatt
     4861 ccaaccctct tgagcgcctg gttgacgcgc tccgattctc ggcgtcctca tttcgcgata
     4921 aagagcacct tgtcggtggc atccaattcg gtctgatgat cgtcggcgac aacgcgcacc
     4981 tctaacatgt gtctgcccgg tttgatgccg ttcagtgtac cgccgttacc ttcgaacatg
     5041 ccgatcaatt caccgtcgat gtaagcgtgg gcatggtggc ctcgattgcc tttggtcagg
     5101 ttaaagcgta gctcaacccg atcgccggtg accgcctgat tattcaccgg cgcgatgatc
     5161 tcgaccttcg cgccttgagc tggttgtaat gctgacgcct gcgccggcgg aaaattctgc
     5221 cgcgccgcgt gcagagcgtg cacccccggc ggaagcaccg ttagatagcc ggtggtggcg
     5281 aagatcgcca ggcccagcat gctttcaacg acgattcgcg tagtcgcctt gtcgactagc
     5341 gagttatttc caacatccgc atgggccatg tcccgaatgg caggccgggt tgacaagaag
     5401 tttatagccc ccagcgtcag catggcggca aacaatccta gtttgacgat cagaacgttg
     5461 cctgagtcgg ttgccaccag tgtcgtcagt gagccgacat gaacccaact tagataaagg
     5521 ccagcgcaga agaacagcgc aacactcaca aaagcgaggc gcgaaaaacg attgatgaat
     5581 tgcgccagcc agacgagact tagatcagcc tgattgcgct tagctcttac ggtagtaagg
     5641 acagcgacta atccaccggc ccataaggcg gtggtaataa gatgtacagc atccgcagca
     5701 atgacccacg aagtgtggag tttgacggcg atagcgtggc tggtcaaact ccggctggcg
     5761 atcaacggca gcgataacag agcgagccag cggatgccgt gcaatcgtgc cgtgccgcga
     5821 gtaatggaaa atccgagcaa ggccaatgca attgcatagc ctgcacgtat gagccaaacc
     5881 ttaccgcttt gggtggcggt cagataacgc cacaggatct caagcgacgg caactcgccg
     5941 tgctggagtt tggcgccttg gatgaccaat gcgcccacag aggcgcagag aaagacgatt
     6001 aagaagcggc aagcgcggat tgccagtgtg cgccggaacg attccgattc gcttcccggc
     6061 agcagaaacc cggcggtgac gaaaaagcct agaaccaaag cctgggcgat ataaccgatg
     6121 gcggagatgg cagcttccaa accgaccatg gcattattca cttgccgctg gggacaattg
     6181 taaaccgcag cacgccttca gtgacgtggg tgtcgacgga cagtacttgc cattccacag
     6241 tatagtttcc ggcttgcagc ggttttaggc cgatggcgag gctgtcgtca gtcaatttgc
     6301 gcagcttgcc ttggctgacg atttccttgt tcggtcctat gaccgtgact gcgctgaagg
     6361 ccatttcaat cgcttcggta aattgaatgc gcacttcgtt cggcgcctcc ttcaatgtcg
     6421 cgccgttgtt aggcacggaa accgccgggt aggcatgcgc ccataccggc ggcgtggccg
     6481 tgacgatgac aaacgcccag aggaaaaaac taaggccgat taaacaccgg aaaaacatag
     6541 gtcgactcat ggctggtttt gtccttctgg ccatcgggcc gggcgctttc ctcatcgatt
     6601 ttgacccggg ccagataaga atccgccggt cccacaggga attggactct accattgtca
     6661 tccgtcgtgc gttcgagcac ggaggcgaaa ccgtcgacgc ggcgcacgag ggtgacgatc
     6721 tgaccgccga tgggtttgcc gcgttgccgc agttctaatg ttatcgaccc gcccgagtaa
     6781 actgccagcg gattattaag cggaatgaat tcggctacgt catcgccgcc gatcgcccgg
     6841 tcaaagcctt tgaggttccg agcagcggct atggtgggta tcttgaatgc ggcaaatggc
     6901 gtcttggcga ctctaacggt taccaccttc ggaccatcgc cctgctgaac caatcgcact
     6961 tgcctggcgt aggcgtggaa taagccatcc gcctgcggcg tgaacggtgc gagaaggaat
     7021 cccttaggtc ctttggggcc gactttttcc tcgtcttcgc ccatgtcgaa taatcgatcg
     7081 gtgagcgaag tttgttttcc gttggcttcg acgaccgcca gggtgacgta ttgagcattc
     7141 catttgccgg cgatgcggta gcttttatgt tcgttggagt gatttccctg gatcaatgca
     7201 atggttgccg gctggttcct ctcgacgatg accggtgcga tttctaccca gccatcgtgc
     7261 gctaccagcg gactctgaaa tcccattaat gcgatgtatc cgtacaaaat gcttcggtta
     7321 attcccttca ttgcgcactg cctcccaagt tgcgaacggc gccgcgccca atggtcgcac
     7381 tgatcggcga ccatcacacc ggctaattta tgcgtcttat tttgaaccgg aagctaggta
     7441 aggggaggcg cgcgaatcga aaatgccgta gtgacgaaat gataaggtgg gatcgaatct
     7501 cgctgaatgg tgctcgtttc ggttgagact tggtgcaagc taaaagtaac ctgagcgaca
     7561 aacctcaagt ggcaacctgc tgccacggat tgaaatacac agggcggttc gttgattggc
     7621 tgatcgtgat ggtccgattg attgtgctga tcgccgtcgt gttcgtgggt ttctgccggc
     7681 tgggcgaaag agtgatgcac gcgatggggc gcgctgtaag cgagaaaaaa aagcaggacg
     7741 ctagctatga ataaacttaa gaatcgccgg gcactagagc attggattgc cgcagtcatg
     7801 ggttacacac gcaaacaaca ctttaacgca caattgcgcg ccgctacaag atcgttttac
     7861 gtaacgaagc tagactgcac agcggctagg aatcccagca tgatctcgaa aaatcgtcgg
     7921 cgacagttcg atttcgccgg aggctacctg atccgccagc cgcaggggcc gaaagcgaat
     7981 ggagactatt attctcccgt ccgtagcttt ttgatgtact ctcgcctctt ggtggtgaat
     8041 cccagccggc cagtatctat tgttaattcc gaaaaccgcc aaaaaccgcg cggttttcgt
     8101 cctgccagaa tatctgcacg tctttgaagc cggcgccgcg cagccaatgc atctgctctt
     8161 gccagggcgg gcttgatcga tcaaagttta gaaaacagcc gcccggcttc accagcgtat
     8221 aagcatcctc gtagataccg cgaataatcc ttgagctgcc cacgttgtgg atcgcaatcg
     8281 acgaaacgat ggcttcgaac ggccccggga ccagtttgct ccagccgtgt ttgccgaaat
     8341 cgcacagaac atattcgaat ctgccggcga gaggtttcat gcgctcgcgg ccgagcttgg
     8401 ccatttcttc tgagccgtcc tggcaaattg ccgtggcgtt ggggaagcgt tcgagcaagt
     8461 acttgcttag cgcgccatag cccgcgccca tgtctaggat attgatcgcg gcggtttttc
     8521 gaagggaatg gtatcggcca gccggctgaa ggccgccgcg cggttggctt cttttggatc
     8581 ctgcccctga gcccatttgg aaacatattc cggcgatgtc caatcatggg gatcgtgatc
     8641 gtgcgcatgt tcggggtcgc cgtgccggtg atgtcccgac gaatgtgttg aatcggtcat
     8701 ggttcgcctc ctgattcgat ttgtcgtgta ttgcacgata gtacgaatta aaaattcatg
     8761 ctactaactt gatataccta cttcggcgcg ccgtgcccag gttgctttcg cgcgcttcat
     8821 cttttgtaac cgatgcgaag atgatcacca cgcgagactc gcggaaaatc ccaatcatgg
     8881 atccggtcga tcaagcaata aatatttggc tttagtacca ataagatact gcactatcga
     8941 tgggacgaga ttctctcacg ttccgctcac taggcgatac gtcgcggtgc gtaactcaaa
     9001 cgagaactat ctactaaggc ggaaccggta gatatagata aattccgcgg gaacattttt
     9061 tccgtctctt tgcgccggct cgaagcgcga ttgcttgacg gctttaagcg cctcctcgtc
     9121 gaaacctgcg ccgccgctcc tggtgatctc cgccctggtt acactgccct gcggatcaac
     9181 ttcgattttt aaggtcacgt cgctttccat gcccgcgcgc agcgccatcg gcggataggc
     9241 agcgcgcacg gtctggatcg gtttggcttc acgattggtt ctaaaaatcg gctcctgcgc
     9301 gggcagacct ggtgcgccag agccgccgcg gcctaaaccg aacggtgcgg tcccgccgcc
     9361 gccaccggcg gtgccggctc cgggcacgac gccgacatca cccttgccaa ataaatttcc
     9421 ggggcccgcc tcgctaccgc caccttcgct gcgggcccca gaagcgtagc ttggaggtgg
     9481 ctcgactttt gccggagcaa gcggttttac ttcgatcgct tttgccggat cttctttggt
     9541 gattggcagc ggcgcttccg gattaaccac cggcgctttc gctaaggcag gcgccgactt
     9601 gggtttctct ggttttggcg gagcggacgg agacggcttt ttaatcgctt tttttgcttc
     9661 ggctttttgc ggcagcgggg cagtttccgg tcgaggcgcg tcgaccaaat cgatgggaag
     9721 caaatcccag tgacgtggag cggtgtggtg aaaaatgaca gaaccgagca acatcaccaa
     9781 gagatggaca attaatgagg cgatgagagg aacgccgagg gtatcatttc tcactgcggc
     9841 tttccttggg ctttatcgcg atagccatct tagcgacgcc ggcttgccgt gcttggtcca
     9901 tgatctcgac cacgaggcca tggtcgactc gctcgtcggc attgatgatg atcaataagt
     9961 ccggagactt gttcagcctc gctttcaaaa tttcgccaac ttgggcgcat ggcacgtcct
    10021 gcatggcaat tgtgtcgtcg gacgcgatgg ttagcgccgc gctttgactc ggagtttgtc
    10081 ggccggtggc caccttcggc aaattaaccg gtagaccgct gtagcatgcc attgatagcg
    10141 tcgagatcat gaagaaaacc agcaaaaaaa gaccgtatcg atcatcggaa tgatctcgaa
    10201 acgaagcttc ttgactctcg ccggttgagg cgcattacat tttctctttg tcgccacccg
    10261 agggccagct caaggcgcga ggcgtatgtt cgatttccat tccacgctcg ccaaaacagg
    10321 caacgctcga tgacaaccgt caatgcgatg accgaacata gcagcagcgg cgccatgacc
    10381 agaccgccct tgatgatgat atcgatgaag tcaaacattt aaagttatcg ctttaggaat
    10441 gcgccggttc agcttcgagc gggcggagcc gcttcaccgg gtcgcggaag aagaggagca
    10501 acgtcatgcc aagaaaatag gaacaagaga tcacgccaaa agtggtttga aatcccgaag
    10561 tgctcatgag ccaaccggcc aaaagcgtcc agaacggcga catgatgaaa ccgatgcaat
    10621 ag
//
LOCUS       DASQKS010000227         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1376731, whole genome shotgun sequence.
ACCESSION   DASQKS010000227 DASQKS010000000
VERSION     DASQKS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376731"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VE170_05920"
     CDS             <1..311
                     /locus_tag="VE170_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15008.1"
                     /translation="RMDDSLDTVAACAHSDKVGSIGTPRGKAEHALVIEWQAAWACLA
                     VSNRVELRRTDGQRRLAADLEGRHRRLLATEDTDPELELIRGADLRPCGDHHGQKART
                     "
     gene            complement(287..1144)
                     /locus_tag="VE170_05925"
     CDS             complement(287..1144)
                     /locus_tag="VE170_05925"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HYJ15009.1"
                     /translation="MAIAYDASRIALLQPERRDTVFRDGPPASAEALAAEAARLAYLR
                     VEESQAERDRLAAALALAGFDPPALFNDPATDGQGYGALRTDGFGLLVFRGTQPDRLG
                     DLATDLGFVFSPLDHGAGQVHAGFRGTALSLWPEVNAWLDGPARRRQGLMVCGHSLGA
                     AIATLLARPAQATHLITIGSPRVGDATFAAGITATLGLEVTRIVDCCDVVTEIPPTQA
                     GFEHAGRLTYIDRNGGVAANAATEFINADRLQARIDYLAQHAFRFGTVLSRDLADHAP
                     VNYVRAFWP"
     gene            1268..1486
                     /locus_tag="VE170_05930"
     CDS             1268..1486
                     /locus_tag="VE170_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15010.1"
                     /translation="MNRPQLMAAWAFILGCACICPWTSFAAEWKDKAEAEGRLVFYTT
                     ANTADTKALTDSDFDRYIKLYQEIFGVK"
     gene            1628..1993
                     /locus_tag="VE170_05935"
     CDS             1628..1993
                     /locus_tag="VE170_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15011.1"
                     /translation="MSIKLNHTIVPAKDKDAAARLFAKIFGLTYDGPMGHFAPVRIND
                     ELTLDFDNADSFEPHHYAFHVSDEEFDAIFQRIQDTGLTWGSEPRSPTNGQLNTRKGG
                     RGIYFHGPDGHLLELLTRP"
     gene            2139..3137
                     /locus_tag="VE170_05940"
     CDS             2139..3137
                     /locus_tag="VE170_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15012.1"
                     /translation="MIYRNIACYCLTVALLFSGVGFAQEKKLDRLRVGGGSASAAQMA
                     IWFAKEGGFYEKHGLNVEVISIPGSSLAIQAMLSGELPIIQAGGAGPIQVALSGTDTV
                     ILATIVKKFNWWIFGQPNIARVEDLKGKIFGVTRFGTQSDLASRIALRKAGLEPERDV
                     TMIQTGGPAETVTAMAAGRVNAAAISPPATLQAKRLKLKDLMDLTTLDVEYHVNGVVT
                     TRRYLKSNEDIVRRFLRAYTEGAVRGMKDKAFAIKTMGKYFRTDDKEVLDETYELSIK
                     TGFTVPPYPAGIAGLLQDLEKTTPKAKTAKPEEFIDNRLVREMDQSGFVKSLLSAK"
     gene            3408..>4154
                     /locus_tag="VE170_05945"
     CDS             3408..>4154
                     /locus_tag="VE170_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ15013.1"
                     /translation="MAQSTDDKFRVTLLGTGAPPPRLDRFGPSTLVEVGQQKFIFDAG
                     RGAMQRLHQLAIPFSDITGLFLTHHHSDHVVGFPDLWLTGWIGRPWGRRAQPLPVYGP
                     VGTRQMMEHLPLAFHVDIRVRSRVYPPDGVKLDACEISQGVVFDHAGVTVSAFEVDHG
                     GEELPAYGYRIDYQGRSAVLSGDTTFNENLIDHAQGADLLVHEVTALAGSAAEDPQQL
                     KRIGANHTTPEQAGEVFARVRPKLAVFNHLL"
BASE COUNT          858 a         1169 c         1289 g          838 t
ORIGIN      
        1 agcgaatgga cgattcgctc gatacggtcg ccgcctgcgc gcacagcgac aaggtggggt
       61 cgatcgggac cccgcgcggc aaggcggaac acgccttggt gattgagtgg caagccgcgt
      121 gggcctgtct ggcggtctcg aatcgggttg agctgcggcg taccgatggg cagcgccgct
      181 tggccgccga tctcgaaggt cggcataggc ggctgctcgc taccgaagat accgatccag
      241 agctggagct catccgcggc gccgacctga ggccatgcgg cgatcatcat ggccagaagg
      301 ctcgcacgta gttgaccggc gcgtggtcgg cgaggtcgcg cgagagcacg gtgccgaagc
      361 ggaacgcatg ctgcgcgagg taatcgatcc gggcctgaag ccggtcggca ttgatgaatt
      421 ccgtcgccgc attggcggcg acgccgccat tgcgatcgat gtaagtcaat cggccggcat
      481 gctcgaagcc ggcctgcgtc ggcggaatct cggtaaccac atcgcaacag tctacgatgc
      541 gcgtcacttc gagccccagt gtagccgtga tgccggctgc aaaagtcgcg tccccgaccc
      601 gaggggagcc gatcgtgatc agatgagtag cctgggccgg ccgcgccagt agtgtggcga
      661 tggcagcgcc caagctgtgg ccgcagacca tcaacccttg gcgtcgccgc gcgggtccat
      721 cgagccaggc atttacttcg ggccacaggc tcagcgccgt tccgcgaaag cctgcatgca
      781 cctgaccggc accgtggtcc aaagggctga acacgaagcc gaggtcagtg gcgagatcgc
      841 caagtcggtc gggttgagtg ccgcggaaga cgagcaagcc gaagccgtcg gttcgaagtg
      901 cgccatagcc ctggccgtca gtagccggat cgttaaagag agcgggcggg tcgaagccgg
      961 ccaacgccaa cgccgcggcc aagcggtcac gctcggcctg tgactcttcc acacgcaagt
     1021 aggcgagcct tgcagcttca gccgcgagcg cttcggcact cgccggcggg ccatctcgga
     1081 acacggtgtc gcggcgttcc ggctgtaaca gtgcgatccg gcttgcatcg taggcgatag
     1141 ccattgcaaa ttgttttact cgaaaacgtg aattcggtaa atatgtcgga gccgtagctt
     1201 cgcactattg tcctagcaga caacgatttg cattcgtggt aaatgagccg gtggaggaac
     1261 agtcgaaatg aatagaccgc agttgatggc ggcctgggcg tttatcttag gatgcgcatg
     1321 catatgccct tggacttcat tcgccgccga gtggaaggac aaagccgagg cggagggaag
     1381 gttagttttc tacaccaccg ccaacacggc ggacacgaaa gccctcaccg acagcgactt
     1441 cgatcgctac atcaagctct atcaagagat attcggcgtc aagtgaaata atgttgggct
     1501 ggcgataggt tttctcacag agcgatcagc gtcgttcgtc agcgggcgaa tcgatcattg
     1561 aatcgagcgt ttaatcggtc ttggaatcgg gcatcgaatc ggtcttcgaa tcggtcttgg
     1621 agggattatg tcgatcaagc tgaaccacac gattgtgccg gctaaagaca aggatgccgc
     1681 ggcgcgcttg ttcgcgaaga tttttggcct cacctacgat ggaccgatgg gccattttgc
     1741 gccggtgcgg attaacgatg agctgacact ggacttcgat aatgccgatt ccttcgagcc
     1801 gcatcattac gcctttcatg tcagcgacga ggagttcgat gcgatctttc agcgcatcca
     1861 agataccggc ctgacctggg gcagcgagcc gcggagtccc accaacggcc aattgaatac
     1921 tcgaaagggc ggccgcggca tatactttca tggccccgac ggccatttgc tcgagctgct
     1981 cacccggccg tgagtgattg ggaaatcacc gcatcctgcc agctcagatt gagtagtgat
     2041 tgcccaactc tgcatggtgc tcgacatcac agcgggtttt catgttatat gaaaagcgaa
     2101 ttgccagttt tgaaaggaaa accggagcta aaataatcat gatttaccgg aacattgctt
     2161 gttattgctt gaccgtggcg ttgttgtttt cgggagtcgg gttcgcccag gagaaaaaac
     2221 tcgaccgcct gcgcgtcggc ggcggttcgg cgtcggcggc gcagatggcg atctggttcg
     2281 ctaaagaagg cgggttctat gagaagcatg ggctcaatgt cgaggtgatc agcattccgg
     2341 gcagttcgct ggcgatacaa gccatgttgt cgggggagtt gccgatcatt caagccggcg
     2401 gcgccggccc gattcaagtt gccctgtcgg gcaccgacac ggtgattctc gcgaccatcg
     2461 tgaaaaaatt taactggtgg attttcggcc aaccgaacat cgcgcgagtg gaggacttga
     2521 aggggaagat ttttggcgtc acccgcttcg gcacgcagtc cgatttggcg tcgcgcattg
     2581 cgctgcgcaa ggccggactc gagcccgagc gggatgtcac gatgattcaa accggtggac
     2641 cggcggaaac cgtcaccgcc atggctgcgg gccgggtcaa cgcggcggcg atcagcccgc
     2701 cggcgacgct gcaagcgaag cgattgaagc tcaaggattt gatggatctg accaccctcg
     2761 atgtcgagta tcacgtcaac ggcgtggtca cgacccggcg ctatttgaag tccaacgaag
     2821 acatcgtccg gcgttttctg cgcgcttata ccgaaggcgc ggtgcgcggc atgaaagaca
     2881 aggccttcgc gatcaagacc atgggcaagt attttcgcac cgacgacaaa gaggtgctcg
     2941 acgaaaccta cgagctcagc atcaagaccg gcttcaccgt gccgccgtac ccggcgggca
     3001 ttgccggtct gttgcaagac ttggaaaaaa ccacgcctaa agccaaaacc gccaagcccg
     3061 aggagttcat cgacaatcgc ctggtgcgtg aaatggatca gagcggcttc gtgaagtcgc
     3121 tgctgtcggc gaaatgagcc gacgatagta aatgcccaag actcacgacg aagacgccaa
     3181 ggccagcaag gttcgaaaaa gttgattctt agctctgcaa ctttgtactg ctatccgagg
     3241 taatttgatg tgccaaagcc aagagggcgg gtttgaaacc cgcccctacg ccccccgatt
     3301 cttattttgc gccctttgcg ttctttgcgg ccattcttct tttcgatcgc ggctttgtcg
     3361 tgttaggtcc ttccttgtgc aatggcattt ctctgtagga actgcggatg gcgcaatcga
     3421 ctgacgataa atttcgcgtg acgctgctcg gcaccggcgc tccgccgccg cgcttggatc
     3481 gatttgggcc gagcacattg gtcgaagtcg gccagcagaa atttattttc gacgccggcc
     3541 gcggtgcgat gcagcggctg catcaattgg ccattccgtt cagcgatatc accggccttt
     3601 ttctcaccca tcatcactcg gatcatgtcg tcggctttcc cgatctctgg ctcaccggat
     3661 ggatcggccg gccctggggg cggagggcgc agccgttgcc ggtgtacggt ccagttggaa
     3721 cacgacagat gatggagcat ttgccgctgg ctttccacgt cgatatccgc gtgcgcagcc
     3781 gcgtttatcc gccggacggc gtcaaattag acgcctgcga gattagccaa ggcgtggtgt
     3841 tcgatcatgc cggggtcacg gtcagtgcgt tcgaggtcga ccatggcggc gaggaattgc
     3901 cggcctatgg ttaccgcatc gattatcaag ggcgctcggc ggtgctttcc ggcgatacca
     3961 ccttcaatga gaatttgatc gatcatgccc agggtgccga tttactggtg cacgaggtca
     4021 cggcgctcgc cggcagcgcg gccgaagatc cgcaacaact gaaaagaatc ggcgccaacc
     4081 acaccacgcc ggagcaggcc ggtgaggtgt tcgcgcgggt gcggccgaag ctggcggtgt
     4141 tcaatcacct actg
//
LOCUS       DASQKS010000228         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2651582, whole genome shotgun sequence.
ACCESSION   DASQKS010000228 DASQKS010000000
VERSION     DASQKS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651582"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VE170_05950"
     CDS             complement(<1..424)
                     /locus_tag="VE170_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15014.1"
                     /translation="MRRGVLTRVIIFSVLALLSALFLAPNFLGKKDSTGRTEMPNWWP
                     DFLPSDAIHLGLDLQGGSHLVLEVKLDKAIENHMERLKSDLTNSIRDKGISGVSVERI
                     EGTQLQVKVATGSVDRVRSILKADFANLSEAKTPLTSAT"
     gene            complement(429..764)
                     /gene="yajC"
                     /locus_tag="VE170_05955"
     CDS             complement(429..764)
                     /gene="yajC"
                     /locus_tag="VE170_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYJ15015.1"
                     /translation="MVEFAYAQAAPGAAGPGPLMTIFPFIIILVIMYFMVFRPQQKKQ
                     KEHQEMLNKIKRNDEVMTSGGIYGKVMEIKDQVVTLEVAPNVRIRVNRPQISAVITAD
                     KASAKDGKD"
     gene            complement(786..1895)
                     /gene="tgt"
                     /locus_tag="VE170_05960"
     CDS             complement(786..1895)
                     /gene="tgt"
                     /locus_tag="VE170_05960"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYJ15016.1"
                     /translation="MVNHFSVVARDLSTKARLGRLTTPHGVVETPCFMPVGTQGTVKA
                     VLPRDLHELGCQILLGNTYHLYLRPGHERIRQLGGLHRFMNWPRPILTDSGGYQVFSL
                     AAMRKITDNGARFQSHIDGSSHLLTPEKAVEIQEALGSDIAMVLDECVPHDAARSYVR
                     ESTERTIRWAERCLNARHRPEQLMFGIVQGGMFEDLRRGCVDALAPMAFDGFAVGGLG
                     VGEGEAKLNDVGAFTVELLPEDKPRYLMGVGRPQDIIHAVRAGFDMFDCVIPTRNGRN
                     GTLFTWQGKVSIKRAEYADDARPLDESCGCYSCGNFSRAYLRHLFMAGEILSAQLNSL
                     HNLYFYHRLMDKCREAIRDGRAELWAKLVNFAEAS"
     gene            complement(2022..3062)
                     /gene="queA"
                     /locus_tag="VE170_05965"
     CDS             complement(2022..3062)
                     /gene="queA"
                     /locus_tag="VE170_05965"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HYJ15017.1"
                     /translation="MNLKEFDYQLPESLIAAFPSRERTGSRLLVARRATGEIIHSAFA
                     ELGSFLDPGDLLVLNDTKVFPARLTGTKDSGGKVEVLLIERFADDSRSLWIAMADAAK
                     KPQVGSLLRFGANMTAEVIGDLGRGRFGLEFHHEGDFDNQLDELGEPPLPPYVRRSRD
                     SESLDRERYQTVYAANRGAVAAPTAGFHFTPELLAGLGAQGIDHTLLTLHVGPGTFQP
                     VRVAEIENHRMEGERYSLSAASAEKIARTKLTGHRVVAVGSTSTRTLEWIALQKGALV
                     ADEGIARLYLRPGDKFRVLDALITNFHLPASTPLILVAAFAGLELVQRAYREAIERHY
                     RFYSYGDAMLIL"
     gene            complement(3369..4106)
                     /locus_tag="VE170_05970"
     CDS             complement(3369..4106)
                     /locus_tag="VE170_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HYJ15018.1"
                     /translation="MATFLVIAVAALISGIKILKEYERGVVFRLGRMVGARGPGMVYI
                     IPGIEKMVKMDLRTVTMDIPPQDVITRDNVSVKVNAVLYFRVLDPNLAVREVENYLFA
                     TSQLAQVTLRSVCGQGELDELLSEREKINSRIQDILDRQTDPWGIKVVLVELKHIDLP
                     QEMQRAMAKQAEAERERRAKVIHAEGEFQASQKLADAAGVMALQPMALQLRYLQSLVE
                     ISSEKNSTTIFPVPIDLLTPFLKKDPP"
BASE COUNT          900 a         1218 c         1132 g          866 t
ORIGIN      
        1 cggtggcgct tgtcagcggc gtcttggcct cggacaagtt agcaaaatcg gctttgagga
       61 tgctgcgcac ccggtcgacg ctcccggtcg ccaccttgac ttgcagctgc gtgccttcga
      121 tccgctccac cgataccccc gaaattccct tgtcgcgaat actgttggtg agatcgctct
      181 tcaaacgctc catgtgattc tcgatcgctt tgtcgagctt cacttcgagc actagatggc
      241 tgccgccttg cagatcgagg ccgaggtgaa tcgcatccga tggcaagaaa tcaggccacc
      301 aattgggcat ttcggtcctg ccggtactat cttttttgcc gagaaagtta ggcgcgagaa
      361 agagcgccga caacagagcc agaaccgaga aaataatcac tcgggtaagc acaccccgtc
      421 gcattcgatt aatcctttcc gtctttggct gatgccttgt cggcggtaat aacggcagag
      481 atttgcggac gattcacgcg gatacgcacg ttcggcgcaa cttcgagggt caccacctga
      541 tctttgattt ccatgacctt accgtaaatt cctccggagg tcatcacctc atcgtttctt
      601 tttatcttat tgagcatctc ctgatgttct ttttgcttct tctgctgcgg ccgaaacacc
      661 atgaaataca tgatgacgag aataatgatg aacgggaata tcgtcatcaa cggtcccggt
      721 ccggcagcgc ccggcgccgc ttgcgcgtaa gcaaactcga ccataattcc tccccaacag
      781 ttttcttatg aagcttcggc gaaattgacg agcttggccc acagctccgc gcgcccgtcg
      841 cgaatcgctt cccgacactt atccatgagg cgatggtaaa agtaaagatt gtgcagcgaa
      901 tttagctgcg ctgagagaat ctcgccggcc atgaacagat ggcgcaggta ggcgcgcgag
      961 aagttgccgc aactgtaaca gccgcagctc tcgtccagcg gccgcgcatc gtcggcatac
     1021 tcggcgcgtt tgatgctgac tttgccttgc caggtgaaca gcgtgccgtt acggccgttg
     1081 cgggtcggga tcacgcagtc gaacatgtca aagccggcgc gcacggcgtg aatgatatcc
     1141 tgcggccggc cgacgcccat caagtaacgg ggtttatcct cgggcagtaa ctcgactgtg
     1201 aaagcgccga catcattgag cttcgcctcg ccctcgccga ccccgaggcc gccgacggcg
     1261 aagccgtcga aagccatcgg cgccagggcg tcgacacacc cgcgccgcag atcctcgaac
     1321 atgccgcctt ggacaatgcc gaacatcaac tgctccggcc gatggcgcgc attcaaacaa
     1381 cgctcggccc agcgaatcgt ccgctccgtc gattcacgca catagctgcg tgccgcgtcg
     1441 tgcgggacgc actcatctag caccattgca atgtcgctgc ccaaagcttc ctgaatctcc
     1501 acagcctttt ccggcgtcag caaatgggac gagccatcga tatgcgattg aaagcgcgca
     1561 ccattgtcgg tgatcttgcg catggcggcg aggctgaata cctggtagcc gccgctatcg
     1621 gtcaagatcg gtcttggcca gttcatgaag cgatgcagcc cgcccaactg gcgaatccgc
     1681 tcatgaccgg ggcgcaaata cagatggtac gtattgccga gcagaatctg gcaccccaac
     1741 tcgtgcaaat cccgcggcaa tacagccttt accgttccct gcgttcccac cggcatgaag
     1801 caaggcgttt cgaccacccc atgcggcgtg gtgaggcgac ccaggcgcgc tttggttgag
     1861 agatcccgcg cgacgacaga gaaatgatta accatcaact tgtggccgat tttcgatttc
     1921 ggtcctagga gccgcttttg cattagcgga ctgccagtga gaggaaaccc acccaaagtt
     1981 gagccgtctg gttctaatgc ggcaatccca aatccaaaat ctcataaaat cagcatcgcg
     2041 tcgccgtagc tataaaaccg ataatgccgc tcaatcgcct cgcgataagc ccgctgcacc
     2101 agttcgaggc cggcaaatgc ggcgacgagt atcagtggcg tcgaggcggg cagatgaaag
     2161 ttggtgatta gggcgtcgag cacgcggaac ttgtctcctg ggcgcaggta cagcctggcg
     2221 atgccctcgt cggcgaccag ggcgcccttc tgcaaagcga tccactccaa cgtgcgcgtg
     2281 ctggtggagc cgacggcgac aactctatga ccggtcagct tggtgcgagc gattttctct
     2341 gcgctggccg cgctgagcga gtagcgctcc ccttccatgc gatgattttc gatctcggcc
     2401 acccgcaccg gctgaaacgt acccggcccg acatgcaggg tcaacaaggt atgatcgatg
     2461 ccctgcgcgc caagcccagc cagcagctcc ggcgtgaaat gaaagccggc ggtaggtgcc
     2521 gctactgcgc cgcggttagc cgcgtagacg gtctgataac gctcgcggtc gagagactcg
     2581 gaatcgcggc tgcgtcttac ataggggggc aacggcggct cgccaagctc gtccagttgg
     2641 ttgtcgaagt caccttcgtg atggaactcc agaccgaagc ggccgcgccc gagatcgccg
     2701 attacttcgg ccgtcatatt ggcgccaaag cgcaatagac tgcccacctg cggtttcttg
     2761 gcggcatcgg ccatggcgat ccataagctc cggctatcgt cggcgaagcg ctcgatcagc
     2821 agcacctcga ctttgccccc gctgtccttc gtccccgtca aacgcgccgg aaaaactttc
     2881 gtatcgttga gcaccaaaag atcgccggga tcgagaaaac tgccaagctc ggcaaacgcg
     2941 ctatgaatga tttcgccggt ggcgcgacga gcgactaaca accgagagcc ggtgcgctcg
     3001 cggctgggaa acgccgcgat caaagactcg ggcaactgat aatcgaattc tttgagattc
     3061 atttaaacgg aacaattagg attttgcggg gatatcggga gtaaagtttt catcacgcat
     3121 cgtgtcataa tcttacgttg ccacagcgtc tgaaagcgga cccagttctc tcgaccgtga
     3181 tccattattt tcgaagaagg gcgagaagcg agcaaagttc ggaattttgg acatcgaggt
     3241 tcgtggtgaa caaaaaattt gaacgacgca ttcgattcac acataagcct gcagagtcgt
     3301 aaaatacggg cacggtgtgc gtactttatg gaactctaat cttccggtgc agctgaagtg
     3361 ctatggcttt atggtggatc cttcttcaag aatggcgtca acaaatcgat cggcacggga
     3421 aagatcgtcg tcgagttttt ctccgaggag atttcaacca aactttgcag atagcgcagc
     3481 tgcaacgcca taggttgtaa tgccatcacg ccggcggcat cggcgagctt ttgcgacgcc
     3541 tggaattcac cctcggcgtg gatgactttg gcgcgccgtt cgcgctcggc ttcggcctgc
     3601 ttggccatgg cgcgctgcat ttcttggggt aggtcaatat gcttgagctc gacaaggaca
     3661 accttgatgc cccagggatc ggtctgccga tccaagatgt cttggatgcg gctgttgatc
     3721 ttttcccgct cggacaagag ttcgtcgagc tcgccctggc cgcagacgct acgcagcgtc
     3781 acctgcgcca gctgggaggt agcaaaaaga taattctcca cttcccgcac cgccagattg
     3841 ggatcgagca cgcggaaata aagcaccgcg ttgaccttca ccgaaacgtt atcacgtgtg
     3901 atcacatcct gcggcggtat atccatggtc accgtgcgca gatccatttt gaccatcttt
     3961 tcgataccgg gaatgatata aaccatcccc ggcccgcgcg cgccaaccat gcgaccgagg
     4021 cgaaacacca cgccgcgctc gtactccttg agaatcttga tgccgctgat caacgccgcc
     4081 accgcgatga cgagaaacgt cgcaatcggt ccgaaa
//
LOCUS       DASQKS010000229         7362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_974671, whole genome shotgun sequence.
ACCESSION   DASQKS010000229 DASQKS010000000
VERSION     DASQKS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974671"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..493
                     /locus_tag="VE170_05975"
     CDS             107..493
                     /locus_tag="VE170_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15019.1"
                     /translation="MVEMKNLVKEADAHPGVGHLSLWRTDLAYYWVINCEAGLQDDMH
                     YHDNDDHIFMCVEGECTVRTPHKAFVLKQFDTVLLQSGESYQLCNTGTGRMILLGAGN
                     SHVDGKPRTRVPNIPSHTPIKEPIVA"
     gene            complement(637..1629)
                     /locus_tag="VE170_05980"
     CDS             complement(637..1629)
                     /locus_tag="VE170_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HYJ15020.1"
                     /translation="MSITKRKSFVMAVGLFALVALTQVALETLADARAGGGRSGGFRG
                     SRSSQAPSRATNPTAPQRQDAMPAQQARQPSSMMPQSGGFMRGLGTAVLGGFLGSMLF
                     SGLAGASGFGGMGGGMGGSGFGMLEILLFGGLAYFLYRKYKASKTPALATSGGSMQYQ
                     DTSYQAPAPGYSNNPPVQEPLPLNSTDYRSLTLMDPSFDANKFLKTAQDNFFKIQGAW
                     NKQDVTALRTLCGGELMKTWEAELAQLKLRGQKNRMDNIALRESEITEVWTESGEDFI
                     TVRLHANLLDFTVDEKSGNVVVGSDSEPVEFEEFWTYSRPVGPNGWKLTAVQQA"
     gene            1803..3044
                     /locus_tag="VE170_05985"
     CDS             1803..3044
                     /locus_tag="VE170_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15021.1"
                     /translation="MAEPKWYQELTTYQWRVLVCACLGWALDIMDGYLYAIILFPAMS
                     DLLGTSEPAVIGWYGGIVLSIFMIGWALGGLIFGPIADRYGRVKTMAITILIYATFTG
                     LCGIANSWWELAIFRFLTGLGIGGEWAAGAALIAESFPERSRAKAAGIMQASGGIGFF
                     LATALYLIIGPYGWRWVFALGVLPAIVAFYVRHSLEEPARWTKAKQTGNPYAALFAPG
                     VRRDLLVGTGLAVVATFGYQGAVQWVPSWIAAMLHAQGTRAVIPQVSLITTTLTTGGI
                     IGCLCMPFIAERWGRKGALFIYFLGALLSVPTTFLFAKEFNHAVLASPVMGFFAAGVT
                     TAFAIYFPELFPTKIRATAQGFCFNFARFFSAAGPLLAGILTSAHGSFAPAIATIGSV
                     YLLGLFILYFARETKGQALPD"
     gene            complement(3160..4431)
                     /locus_tag="VE170_05990"
     CDS             complement(3160..4431)
                     /locus_tag="VE170_05990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15022.1"
                     /translation="MPKLSSLRTLRSLRHRNFRLLMEGALVSSSGDFMQNIAQSWLVW
                     HLSQSPVAIGVIAVFDTVPRLLVGALGGAIADRFDRRRVLMITQSLAMLQAFVFWVLV
                     EFEGIELWHIALLAFFLGVVNTINQTARQSLVNSLVPKEELLNAIGLQSSVFNFSKIM
                     GPSVGGLIIAYVGIAGCFLVNALSFGYLIFNLYQLDLPPWESRQHKHGLLGDIKEGYV
                     YLSGNRRMLGIVGISYVMALFGAPYNRFVPMFATNVLHVGASGFGMLMSAPGIGATVA
                     SLSLATVNKLRVGTRSICFCVLGFALFLTLFAFSHSFILSIIFLAMVGFCQVGERALT
                     NTVIQTGTPQELLGRVLSLFFMDRGLWSLGGLIIGTSASAIGIDWTFAVCGVVCTLAA
                     TALLIFSRRQRTEALQHEGARVLPPVVEEEK"
     gene            4702..5211
                     /locus_tag="VE170_05995"
     CDS             4702..5211
                     /locus_tag="VE170_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15023.1"
                     /translation="MPIVNLAEISQQLAESGKRVRVLWQHNDILTFVARGREYRSEFH
                     INPSDETMYMIKGEMRLHYRTPEGKEEVAVIGEGAMIYTPAGTPHSPRFPADAFALIS
                     ERKRRDGEIDSFHWYCQKCDTHLHEERFIVNDYGEDPVSKAYQRFFESLEFRTCKQCG
                     EIMPAPDAL"
     gene            complement(5461..6921)
                     /locus_tag="VE170_06000"
     CDS             complement(5461..6921)
                     /locus_tag="VE170_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15024.1"
                     /translation="MVPPINKFSSPIELTRRQFFRTAAGGIASIAVADNAVHSAQPKR
                     SLPDKTGRMLLKGGVVLTFDAASGDFEKADVLIEGKKIVAVQPNISATAMIIDASNMI
                     VLPGFIDSHHHCYQGALRNIQTNGLLDDYSRDIVGEATPFYRPEDAYVGELISAVRAL
                     DAGITTVMDISQVSNSPEHSDACIKGLQDGGVRAVFAYARGFGPKAQYPQDIERLQKR
                     YFSTADQLLTLAMGTQLTEEQWAVARKAGVRIFTHVVGRAACDTILKWGQAGLMRADN
                     VYIHATSASAEFMRKTAESGGSVSIAAPIEMTMRHGMPPLQLALDNGIRPSLSSDVET
                     TMAADMFTQMRSVFTLQRAFVNERAIAGEKNLPAMLTAREVIGFATVEGAKCTGLDRK
                     VGTLTPGKEADVVMLRTDLPNVLPFNNAYGAIVAHMDTSNVDTVLVAGKVMKRAGKLV
                     GVDMKHIGKLAERSRNYIVGKTGWPRSVLDTTRPGR"
     gene            7104..>7362
                     /locus_tag="VE170_06005"
     CDS             7104..>7362
                     /locus_tag="VE170_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008079011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ15025.1"
                     /translation="MAIELYWGSGSPFAWRVMLTLEVKGLAYESKLLEFSKGEHKTPA
                     YLQLNPRGKVPTLKDGAFVVYESLAIMSYLDRKYPTPPLFGT"
BASE COUNT         1641 a         2176 c         1987 g         1558 t
ORIGIN      
        1 cacccagagc gcacgggtaa tgatagcatg tgaccgattg aacccttgaa ccttgaactc
       61 ttgaacgaat cactgaaaca ttggcacaaa accgaggagg ttatttatgg tcgaaatgaa
      121 aaatttagtt aaagaagccg atgcccatcc tggcgttggc catttgagtc tctggcgcac
      181 ggacttggcg tattactggg tgatcaactg cgaggccggc ctgcaagacg acatgcacta
      241 tcacgacaat gacgatcaca tttttatgtg cgtcgaaggc gagtgcaccg tgcgcactcc
      301 gcacaaagcc ttcgtcctga agcagttcga taccgtgttg ctgcaatccg gcgagtccta
      361 ccaactgtgc aataccggca caggccgcat gattctctta ggcgcgggca acagccatgt
      421 cgacggcaag ccacgcaccc gcgtgcctaa tatccccagc cacacaccga tcaaagaacc
      481 aatcgtcgcc taggtcgcgg ggcgaagagg gactcttgct agtccggccg catacgccgg
      541 aaactgacag ctgtcggcaa accagcccgg acaaaaaaaa ccggcagtgc atgacctcac
      601 gcactgccgg ttgtaaccaa aatttacttc ttagctctag gcctgctgta ccgccgtaag
      661 cttccacccg ttcggtccaa ccgggcggct gtatgtccaa aattcctcga attccaccgg
      721 ctcgctgtcg ctgccgacga ccacattgcc gctcttttcg tcgacggtga aatcgagcag
      781 attggcgtgc aagcgcaccg tgatgaagtc ctcaccactc tcggtccaca cttcggtgat
      841 ttcgctctcg cgcagggcga tgttgtccat acggttcttt tgtccgcgca gtttgagctg
      901 cgccagctcc gcttcccagg ttttcatcag ctcgcccccg cacaaggtgc gcagcgctgt
      961 cacatcctgc ttgttccacg cgccctggat cttgaaaaaa ttatcctgcg cggttttcaa
     1021 aaacttattg gcgtcgaagc ttggatccat caaggtcaac gagcgatagt cggtgctatt
     1081 taacggcaac ggttcctgca ccggtggatt attagaatac cccggtgccg gcgcctggta
     1141 ggaagtgtct tgatactgca ttgagccgcc gctagtcgcc agcgcaggcg ttttggacgc
     1201 tttgtatttc cggtaaagaa aatacgccaa cccgccgaac aaaagaattt ctaacatgcc
     1261 gaatccactg ccgcccatgc cgccacccat gccaccgaag ccgctggcgc cggctaagcc
     1321 cgagaacagc atcgatccta aaaagccgcc gagcaccgcg gtgcctagtc cacgcatgaa
     1381 accgcccgat tgcggcatca tcgaagacgg ttgacgcgcc tgttgtgccg gcatggcatc
     1441 ctgtctttgc ggcgctgtcg ggttggtcgc ccggctcggc gcctgggaac tgcgggaacc
     1501 gcgaaagcca cctgaccggc caccgccggc gcgggcatcg gctaacgttt caagggcgac
     1561 ttgggtcagc gcgacaagtg cgaaaagacc caccgccatg acaaatgatt tacgcttggt
     1621 tatcgacatg ggttaattcc ctcctaagga attcattcta atcgcaggca gtctaaccat
     1681 gggtagaaat cgagtcaatg atctattttt aataaatccg cggccacaaa atgccggcca
     1741 aacacatccc acgcgagctt ttttattgcc tttctcaacc ctttgtggca cttgagcaaa
     1801 ccatggctga acccaagtgg taccaagagc taacaaccta tcaatggcgc gtcctcgtct
     1861 gcgcctgcct cggctgggct ctcgacatca tggacggcta cctctatgcc ataattctat
     1921 tcccggcaat gagcgacttg ctcggcacca gcgagccggc ggtgatcggc tggtacggtg
     1981 gcatcgttct gagcatcttc atgatcggct gggccttggg cgggctgatt ttcggaccca
     2041 tcgccgaccg ttacggccgg gtcaaaacca tggcgatcac tatattaatc tatgcaactt
     2101 tcaccggact gtgcggcatc gccaatagct ggtgggaact cgcgatcttc cgcttcctca
     2161 ctggcttagg catcggcggc gagtgggccg ccggcgccgc gctcatcgcc gagagttttc
     2221 cggaacggtc gcgcgccaaa gccgccggca tcatgcaagc ctcaggcggc atcggattct
     2281 ttctcgccac ggcgctttat ctaatcatcg gcccctacgg ttggcgttgg gtattcgctc
     2341 tcggcgtcct gcccgccatc gtcgcctttt atgtgcgtca ctcgctggaa gaaccggcgc
     2401 gctggaccaa agccaaacag actggcaatc cctacgctgc tttattcgct cccggggtgc
     2461 gtcgcgactt gctggtcggc accggtctcg cggtagtcgc caccttcggt tatcagggcg
     2521 cggtgcaatg ggtgccgagt tggatcgccg ccatgctgca tgcccagggc acccgcgcag
     2581 tgatcccaca ggtaagctta atcacgacga cgctcaccac cggcggcatc atcggctgtc
     2641 tgtgcatgcc gttcatcgcc gagcgctggg gcagaaaagg cgcgctgttt atttacttcc
     2701 tcggcgccct gctcagcgtg ccgacgacgt ttttgttcgc caaagagttt aaccacgccg
     2761 tcttggcctc gccggtgatg ggtttcttcg ccgccggcgt caccaccgcc ttcgcaattt
     2821 acttcccgga actgtttccg accaagatcc gcgctaccgc ccaaggtttt tgcttcaact
     2881 tcgcccgctt cttctccgcc gccggcccgc tgctcgccgg catactgaca tcagcccacg
     2941 gctccttcgc gccggcaata gccaccatcg ggtcggtata tctcctcggc ttgttcatct
     3001 tgtattttgc ccgcgaaacc aagggccaag ctttgccgga ttaaattgat cggctggcaa
     3061 cgatggggct gcgattcgat aggtgatacg agagcaaact ttggcgaggt ttgagactgc
     3121 cgggctaaac aatttcggcc ggagaaaatt cgtatgcggt catttctctt cctcgacgac
     3181 cggcggtagc acgcgcgcgc cttcatgttg aagcgcttcg gtgcgctgcc tgcggctgaa
     3241 aatcaacaaa gcagtcgcgg cgagcgtgca aacaacgccg caaaccgcga aggtccagtc
     3301 gataccgatg gccgaggccg aggtaccgat aatcaaaccg ccgagtgacc ataagccccg
     3361 gtccatgaag aacaggctca agactcgccc taataactcc tgcggcgttc cggtttgaat
     3421 caccgtattg gtcaaagcgc gctcgccgac ctggcaaaat cctaccatag ccagaaaaat
     3481 gatcgacaaa ataaacgaat gggaaaaagc gaacagcgtc aaaaacagcg cgaatccgag
     3541 cacgcaaaaa cagatagagc gcgtcccgac ccgcagctta ttcacggtgg cgagagacaa
     3601 ggatgccacg gtggcgccga tccccggcgc ggacatgagc atgccgaatc ccgacgcgcc
     3661 gacatgtaag acattggtcg cgaacatcgg cacaaaccgg ttatacggcg cgccaaacag
     3721 ggccatgacg taggaaattc cgacgatgcc gagcatgcgg cggttgccgc tcagataaac
     3781 gtaaccttcc ttgatatccc ccaacagtcc atgcttgtgc tgtcgcgatt cccatggcgg
     3841 caggtccaac tgatagagat tgaaaatcag gtagccaaaa ctgagcgcgt ttacgagaaa
     3901 acagccggcg atgccgacgt aggcgatgat caagccgccc accgacggtc ccatgatttt
     3961 cgaaaagttg aacaccgagg attgcaggcc gatagcgttg agcaattcct ccttgggcac
     4021 cagactattg accagggatt ggcgtgccgt ctgattgatc gtgtttacga cgccgagaaa
     4081 gaaagccaac agcgcgatat gccagagctc gatcccttca aattcaacca acacccagaa
     4141 aacaaacgcc tgcaacatcg ccaagctttg cgtgatcatc aacacgcgcc gtcgatcgaa
     4201 ccggtcggcg atcgccccgc ccagagcacc gaccaatagc cgcggcaccg tgtcgaatac
     4261 cgcgatcacg ccgatggcga ccggcgattg cgataggtgc cacaccaacc agctctgcgc
     4321 gatgttttgc atgaaatcgc ccgagctgga gaccagggcg ccttccatca ataatctgaa
     4381 gtttcgatgg cgcagagaac ggagagttct caaagacgac agcttaggca tgaccgtaga
     4441 caaatctcgg gagcgataac tctattgtta gttgaaatgt tccgacaacg caaaacgtgc
     4501 cgcgcaccgg tagcgtatcg gttgaatcgt cctggcatcg atcatagagt ttgtacctct
     4561 gagacagctt aaggagagcg caccgatgct tcgcgcgccc atttcgggaa tttcgtgcct
     4621 atcatggttg gctcttccaa tccgaacgtt gaactattga accttgaacc tttgaaccaa
     4681 ggagccacgc cgatgttctc catgccgatc gtaaatctcg ccgaaatctc acagcaactt
     4741 gcagagagcg gcaagcgtgt ccgagttttg tggcaacaca acgacatact aacgttcgtc
     4801 gcccgcggcc gtgaataccg cagtgagttt cacatcaacc cgagcgacga gaccatgtac
     4861 atgatcaaag gtgagatgcg cctgcactac cgcaccccgg aaggcaaaga agaagtcgca
     4921 gtgatcggcg agggcgcgat gatctacacc cccgcgggca cgcctcactc accgcgattc
     4981 cctgccgatg ccttcgcgtt gatctcagaa agaaaaagac gagacggaga gatcgacagc
     5041 tttcattggt actgccagaa atgcgatacg cacctgcacg aagaacggtt tatcgtaaac
     5101 gactacggcg aagatccggt gtcgaaagcc taccagcgat tttttgagag tttggaattt
     5161 agaacctgta agcaatgcgg cgaaatcatg ccggcgccgg atgcgctctg acgacaggct
     5221 caggaggcaa acccgacacc gacctcggtt ccatcgcgga tggcgatcca gcattgtcca
     5281 tggcactttt cgtcgactct atcttcgcag cttggaccac aaggggctaa gaagaagaaa
     5341 ttacttttta gtgcttggcg aattaatttc gctcacaagc cgccgggctt cagcgtccga
     5401 atgcgactaa accaaaattg ctgccagctg actcgcatac tgataaccag ggaagaagta
     5461 tcaccgtccc ggtctggtgg tatcgagcac cgagcgcggc cagccggtct tgccgacgat
     5521 ataattgcgt gagcgctcgg caagtttgcc gatatgtttc atgtcgacgc caaccaattt
     5581 gccggcgcgc ttcattacct tacccgcgac cagcacggta tcgacgttac tcgtgtccat
     5641 atgcgcgacg atggcgccgt aggcgttatt gaaaggcaag acgttgggta aatctgtgcg
     5701 cagcatgacg acatccgcct ccttgcccgg cgtcaaggtg ccgaccttac ggtcgaggcc
     5761 ggtgcatttg gctccctcca cggtggcaaa gccgattacc tcccgcgccg ttaacatggc
     5821 cggcagattc ttttcgccgg cgatggcccg ctcgttgacg aaggcccgtt gtaatgtgaa
     5881 caccgagcgc atctgcgtga acatatctgc cgccatggtg gtctcgacat cgctgctgag
     5941 actcgggcgg atgccattat cgagcgctag ctgcagcggt ggcatgccat ggcgcattgt
     6001 catctcgatg ggcgcagcga tcgacacgct gcccccgctt tcagcggtct tgcgcatgaa
     6061 ttccgcgctg gcgctggtgg catggatgta aacgttgtcc gcgcgcatga gaccagcttg
     6121 gccccatttg agaatggtat cgcaggcagc gcggcccacc acatgggtaa aaattcgcac
     6181 gcccgctttg cgcgccactg cccactgttc ctcggtcaac tgcgtgccca tcgccagagt
     6241 cagcaactga tcggccgtgg aaaaatagcg cttctgcaaa cgctcaatat cctgcggata
     6301 ctgcgccttg gggccaaaac cacgtgcgta ggcgaatacc gcgcgtacgc cgccatcttg
     6361 caagcctttg atacaggcat cgctatgctc cggcgagttc gacacctgtg agatatccat
     6421 caccgtcgtg atgccggcat cgagggcgcg cacggcactg atgagttcgc cgacataggc
     6481 atcttctggc cgataaaaag gcgtcgcctc gccgacgatg tcgcgcgagt aatcgtccag
     6541 caggccgttg gtttgaatgt tgcgcagtgc gccctgataa caatggtggt gcgagtcgat
     6601 aaagcccggc aaaacgatca tattcgaagc gtcgatgatc atcgccgtcg cgctgatgtt
     6661 cggttgcacg gcgacgatct tttttccctc gatcaagaca tcggcctttt caaagtcgcc
     6721 gctggccgca tcgaaggtca gcaccacgcc gcctttgagc agcatgcgtc cggtcttgtc
     6781 cggaagactt ctcttaggct gcgccgagtg aacggcgtta tcggccacgg cgatactggc
     6841 aatcccaccg gcggcggtcc tgaaaaactg gcgccgggtc agttcgatcg ggctgctaaa
     6901 cttattgata ggtggaacca tgcgaactcc aaactcttaa agtggggttt ttgactattt
     6961 ccaggctata actcaacggc catcgatggg gcaagttttt tgcgcgggtc aagggattcc
     7021 ctattacagg aattgtcctt cctgtgagtt gtcattgctt gcttaaggga gtttaactgg
     7081 tccgaccaag gaggattaat attatggcca tcgaacttta ctggggcagc ggcagtccgt
     7141 ttgcctggcg tgtcatgctg accttggagg tgaaaggctt ggcttacgag tcgaagcttt
     7201 tggagttttc caagggcgag cacaaaacgc ctgcttatct gcaactcaat ccgcgcggca
     7261 aagtgccgac gctcaaagac ggcgccttcg tagtatacga gtcgctggcg atcatgagct
     7321 acctcgatcg caagtatccg accccgccgc tgtttggcac ta
//
LOCUS       DASQKS010000230         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2221774, whole genome shotgun sequence.
ACCESSION   DASQKS010000230 DASQKS010000000
VERSION     DASQKS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221774"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1479)
                     /locus_tag="VE170_06010"
     CDS             complement(172..1479)
                     /locus_tag="VE170_06010"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYJ15026.1"
                     /translation="MKLEIAEFPVKQIRLSHRFSYENQVLEVDEAELLALVRDDARIE
                     DVSLAVAAPGEKTRITGIRDVVEPRCKVSGGGQVFAGVLGAVEDVGTGRTHRLSGMTV
                     IAAAAYEGTIRAGTTVQRSAILDMSGPGAEVSRFSSLRHLVLSFKIVDGLAELDAHTA
                     IQLAEFKVAERLARITAELATAQMTTYDLTATDSRLPNVMLIQGVITDPQHVHSGVGY
                     YGLSLRSSLATYVHPNELFDGAVTVDTTRSGRGYYPATWDWQNHPLVLALYADHGKTL
                     NFSGVVLQRIRFETSHGKEVGAQNAARLAKAMQANGALVTWIGGGNAFVDVMFTLRAC
                     ERQGISTTLVTYENGGKAGTDSPVLFNVAEADAAISTGSLDRPIELPAMDKVIGPYRQ
                     IKIFPFPGAAPVDARAALPLEARDAMIGGADLWGQGLSKCVDY"
     gene            complement(1570..2586)
                     /locus_tag="VE170_06015"
     CDS             complement(1570..2586)
                     /locus_tag="VE170_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15027.1"
                     /translation="MTLSTPLQAATVAEVAAKIKSLRAQERIAYLLKGAQAEGELAYY
                     GTLPIDEFLPLARVYNSRYRAMELNHYFSPRDGILNRTLTETRAGRHAVDVVQVDLSY
                     GYQLLNANLVQAYQVPGANRYFDGTFDPGGNWHSMYYLTTALIYNSAAVKAEQAPKSY
                     EELLSPSWKGKMLFDPEAGYILAAMEEAWGRDKAVNYLSRLAKQDLSYRRGGTLTTQV
                     VTSGEFPIGIAINGETSAAIREKGAPLGFKVLAPAIVKPEGLFVMKNAPHPHAALLFV
                     EWVLSDEAQNFLAGTLGKGSAMKGVRSKFKEFQVKPDYVVNPKLGANLATYIQDFRKI
                     VGAP"
     gene            2906..>3824
                     /locus_tag="VE170_06020"
     CDS             2906..>3824
                     /locus_tag="VE170_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ15028.1"
                     /translation="MSQWNEEVDLICVGSGISGCAAAIAAAAAGLRVMVLEKSHKLGG
                     TTTWSLGIVWVGNGHLARASGVADSAAETRAYLEFLGGGRNDPAVTQSYVDHAPAALH
                     FFAETAQVPFYMIDKLPDHYYPAGAGSLPFGRSHQVRPFAASSLGSWQTNLDITPYRH
                     GRVTFEEMTARGGMAGQWDKALLAEREAKDIRTFGGALAGYFLKAAIDLRIPIRIETE
                     VARLLVDAGLVVGVEINCRDKKTAIGASCGVVLATGQYDSNPRLMNLFDEFNSWPPRG
                     APRNQGDGLVLAAEHGAALKVMHGNLSILL"
BASE COUNT          805 a         1188 c         1047 g          784 t
ORIGIN      
        1 tctcaagcgt tgctgcgcac aagcaaaaat atttgcgccg caagttttga cagacaatcg
       61 cgattctgtc ggcaatgcgc aacatacacc cggagaagtc ggcagactca cttagcaatc
      121 tttgcgtccc gatgatcctg agcaaggtca aagatgcggc aatccgcaag ctcaatagtc
      181 cacgcatttc gacaatccct ggccccaaag atcggcgccg ccgatcatgg cgtcgcgggc
      241 ttccaatggc agcgccgcgc gcgcgtcgac cggcgccgcg cccgggaatg gaaatatctt
      301 gatctgccgg tacggtccga tcactttgtc catggcgggc agctcgatgg gccgatccag
      361 gctgcccgtg ctgatcgccg cgtccgcctc ggcgacgttg aacagcaccg gactatccgt
      421 gccggctttg ccgccgtttt cataggtcac cagcgtcgtg ctgatcccct gccgttcgca
      481 cgcccgcaag gtgaacatga catcgacaaa ggcgttgccg ccgccgatcc aggtcaccag
      541 cgcgccattg gcttgcatcg ccttggccag gcgcgccgcg ttttgcgcgc caacttcttt
      601 gccatgggaa gtttcgaagc gaatgcgctg caacaccaca ccagaaaaat tcaatgtctt
      661 gccatggtcc gcgtataacg ccagcaccag cggatggttc tgccaatccc atgtcgccgg
      721 atagtaaccg cgccccgagc gcgtcgtatc gacggtcacc gcgccgtcga atagctcgtt
      781 gggatgcaca taggtcgcca gcgaactgcg cagcgacaag ccgtaataac cgacgcccga
      841 gtgcacatgt tgcggatcgg taatcacccc ttggatcagc atcacgttgg gcaggcggga
      901 atcggtggcg gttaaatcat aagtcgtcat ttgcgccgtt gccaattcgg cggtgattcg
      961 cgccaagcgt tcggcgactt tgaactccgc caattgaatc gccgtgtgcg catcgagctc
     1021 cgccagcccg tcgacaatct tgaaactaag gacgagatgc cggagcgaac tgaaacgaga
     1081 cacctcggcg ccgggtcccg acatgtcgag gatcgcgctg cgctgcaccg tcgtgccggc
     1141 gcgaatcgtc ccctcgtaag ccgccgccgc aatcactgtc atgcccgata gccgatgggt
     1201 ccgccccgtg ccgacgtctt cgaccgcacc cagcaccccg gcaaaaacct gcccgccgcc
     1261 ggaaactttg catcgtggtt cgacaacatc gcggataccg gtgatgcgcg tcttctcccc
     1321 cggcgccgcc accgccaacg agacgtcctc gattcgcgcg tcgtcgcgca ccagcgccag
     1381 gagctccgct tcatcgacct cgagcacctg gttctcatag gagaaacgat ggctcaagcg
     1441 aatttgcttt accggaaatt ccgcaatctc aagtttcatg tccgtttaag gttcgattct
     1501 cgcattgatc gttcgagatc cgtccaaacg ttttgaacgg ctggaacgat tggaacgtgt
     1561 tgctaaaaat tatggcgccc ccacaatctt gcgaaagtcc tgaatataag tggcaagatt
     1621 cgcccccagc ttgggattga ccacataatc gggcttcact tggaactctt tgaacttgct
     1681 gcgcacgccc ttcatggcgc tgcctttgcc caaggtcccg gccaggaagt tttgtgcctc
     1741 gtcggacaga acccattcga cgaacaacag cgccgcatgg ggatgcggcg catttttcat
     1801 gacaaacaac ccttccggtt tgacgatcgc cggggccaag actttgaaac ccagcggcgc
     1861 gcccttttcg cggatcgccg ccgaagtctc gccgttgatc gcaatgccga tgggaaactc
     1921 gcccgaggtc accacttgag tcgtcaaggt cccgccgcgc cggtaactca agtcctgctt
     1981 cgccaggcgc gacaaataat ttaccgcctt gtcgcggccc cacgcctctt ccatggccgc
     2041 tagaatatag cccgcctccg gatcgaagag catcttcccc ttccacgatg gactgagtag
     2101 ttcttcatag ctcttaggcg cctgctccgc tttcaccgcg gcgctgttgt agatgagagc
     2161 cgtggtcaaa taatacatgc tgtgccagtt gccgccggga tcgaaggtgc catcgaaata
     2221 acggttggca ccgggcacct gatacgcctg taccagattg gcgttgagca actgatagcc
     2281 gtagctcaag tccacttgca ccacatccac cgcatgccgg ccagcccgtg tttcagtcaa
     2341 ggtgcgattg aggatgccgt cccgcggcga aaaataatga ttcagctcca tggcgcgata
     2401 gcgcgaattg taaacccgcg ccaacggcaa aaactcatcg atcggcaagg tgccgtaata
     2461 ggccagctct ccctcggcct gcgcgccttt aagtaaatag gcgatgcgtt cttgcgccct
     2521 gagcgactta attttcgccg ccacttcggc gaccgtcgcg gcttgaagcg gagtcgacag
     2581 cgtcacgcaa acacttaagg cgctgaaaca agcgaaaaca actgcacagc aacgtctcat
     2641 caaaagctcc tgttctcttt ggccaacgga ctattatctg cttccaccga gcctggttgt
     2701 acaaaacagc cgcgcgaggg tcaaactacc cgaaacctac acgatgacgt tcatagcaca
     2761 gctatcccgc agcggcggga caactatgac cagtagaaat cctcgacgcc atagttgttg
     2821 ccaatcacct ggtcccaagt tagacattcg gaatagcggc cagcgccgtt tcttccggct
     2881 agtttgatcc gaggaggata ttatcttgtc tcaatggaat gaagaggtcg atctgatctg
     2941 cgtcggctca ggtatcagcg gctgcgccgc cgcaatagcc gcagctgccg ccggccttag
     3001 agtgatggtc ctcgaaaagt cccacaagct gggcggcacg acgacctggt ctttggggat
     3061 cgtctgggtg ggtaatggtc acctcgcgcg agccagcggt gtcgccgata gcgcggctga
     3121 gacacgcgcg tacctcgaat ttctcggcgg cggtcgcaac gatccggcag tcactcaaag
     3181 ctacgtcgat cacgcgccgg cagcgctgca ttttttcgca gaaaccgcgc aggtgccgtt
     3241 ctacatgatc gacaaattac cggaccacta ttaccccgcc ggcgcaggat ccctgccgtt
     3301 tggcagaagc catcaggtgc gcccgtttgc agcgtcgtcg ctgggcagtt ggcaaacgaa
     3361 tttggatatt accccttatc gtcatggccg tgtcaccttt gaagagatga ccgcccgcgg
     3421 cggcatggcg ggacaatggg ataaagcgct gctcgccgaa cgcgaggcaa aagatattcg
     3481 caccttcggc ggcgcgcttg cgggttattt tctcaaagcc gccatcgacc tgcgcatacc
     3541 gatcaggata gagactgaag tggctcgctt gctcgtcgat gccggcctcg tcgtaggcgt
     3601 ggaaataaat tgtcgtgaca aaaagacggc gattggcgct tcatgcggcg tagtcttggc
     3661 caccgggcag tacgattcga atccgcgtct catgaacctc ttcgatgaat tcaattcgtg
     3721 gccgccgcgc ggcgcgccgc gtaatcaagg tgacggcctc gttctagcgg cagaacatgg
     3781 cgcggcgctc aaggtcatgc acggcaatct ctctattctc ctcg
//
LOCUS       DASQKS010000231         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_974906, whole genome shotgun sequence.
ACCESSION   DASQKS010000231 DASQKS010000000
VERSION     DASQKS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974906"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VE170_06025"
     CDS             complement(<1..944)
                     /locus_tag="VE170_06025"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HYJ15029.1"
                     /translation="MIVANMYRPYVQPYLRPMWPPWLAPYVGEAMTRVEANKQAIALL
                     GGSVSHSRFVRGGVRKYDSDMGAARFRVAVTGANGKGVLQAALGQVDGTWVFSELRLI
                     LDSGGKVVDLLDGADQPVRAVLETGRQVYLVPLGDVDQEAIGLKELPEFYRKKFNLEV
                     TLLKPLPIEEKAWDRKRRQLIAEELVELMLRRLPHLAKDSKAMVIGVTREDMYYREKN
                     WNFAYNFWSNQKSGLVSSHRIDVQSSSLAARQQVKSRVRKLTSRVVGMLVYELPRSQD
                     PTSVLAKELYGSFSIDLMSDEFDGLGSLAVIDGFTRMH"
     gene            complement(1308..1742)
                     /locus_tag="VE170_06030"
     CDS             complement(1308..1742)
                     /locus_tag="VE170_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ15030.1"
                     /translation="MDWIFQTYDTYAFLFLRFGLAVTFFMHGTQHAFGWHGGKGPKAQ
                     VTNWRDKYGIPLPMGVLALIIELSTVVCMVLGFFVKPVAFGLAVFMAFATFLSHWEHG
                     FFLSSAAGKGSGIEYTMALMLMALTIAVGGAGALSVDMLLSR"
     gene            1905..2972
                     /locus_tag="VE170_06035"
     CDS             1905..2972
                     /locus_tag="VE170_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15031.1"
                     /translation="MQIIDADGHINDHICGEEIAKYMPKGNQMAKLFPEFDHLHFRYL
                     KENRRATGNPDAAAWSEFLEMTGISWTVLYPTAGLAVGRIMAPDWAVIACQAYNSWLH
                     EKFLTKNPRLKGVALIPFQDVDAAIVELRRAVKELGMLGGMLPSNGEAIQGHFGNKIY
                     WPLYAEAEKLDCCLAVHVGALHHMGMDAFTTYYPVHALGHPFSLMIQAAAMIGHGVFD
                     RFTQLRVGFLEGGATWVPFMMDRLDRSYHDGHVQTDVHGNRLGGPAPGEKASDYFNTQ
                     LREGRIFVGFDCDDDGLNTAIAKGGRQSFLFGSDFPHEVFDAGKCRHEIDELLARDDL
                     TQDDKEAVLGGNAMSFYRLGM"
BASE COUNT          792 a          963 c          825 g          650 t
ORIGIN      
        1 cagtgcattc tggtgaaacc atcaattacc gctaaggaac caaggccgtc gaactcatcc
       61 gacatcagat cgatgctgaa ggaaccgtac aactctttgg ccaagacgct tgtaggatct
      121 tgactacgag gtagttcgta aacaagcata ccgaccaccc ggctggttaa tttgcgcact
      181 ctagacttca cctgttgtct tgcggccaat gaggacgatt gcacatcgat ccgatggctc
      241 gataccaaac cggacttttg gttcgaccaa aaattgtatg cgaagttcca attcttctcg
      301 cgatagtaca tatcctcgcg ggtaacaccg atgaccatcg ccttggaatc cttcgccaaa
      361 tgcggcaaac ggcgcagcat gagttcgacc aactcttcgg caatcaactg tcggcgtttt
      421 ctgtcccatg ccttttcctc gatcggcagg ggcttgagta aagtaacctc aagattgaat
      481 ttctttcggt aaaactccgg caactccttt aatccaatcg cctcttggtc aacatcgcca
      541 agcggcacca gataaacttg acgaccggtc tcaagcacag cgcggaccgg ttgatccgca
      601 ccatccagta gatcaacaac tttaccgcca ctatcgagaa tcagccgcaa ttcggaaaat
      661 acccaggtgc catcaacctg cccaagcgcg gcttgcagca cacctttccc gttcgcgcca
      721 gtcaccgcca ctcgaaaccg cgccgctccc atgtcgctat cgtatttgcg tacgccccct
      781 ctgacgaatc gactatggct tacggaaccg cctaacaatg ctatcgcttg tttgttcgct
      841 tcgacgcggg tcatcgcctc gccgacatag ggtgccagcc aaggtggcca catgggccgc
      901 aaataaggct gaacgtacgg gcggtacatg ttggcgacga tcaccaaaag cactgcgata
      961 atcgcatgac taatctttct acctgtcgac cagcggggtt taggaagagc gttagttggc
     1021 gaacgcggca ccacggtacc gcaggccggg cagacatccc gtccggggct tagttctgcc
     1081 gtgcagctac ggcagcgctc gaatggtccg gcgacaccat tagtcgacat gcggcaaatt
     1141 gtaacccgct agcgacattc tgaacagcaa tagtagatga agtgggttag accgaaaaat
     1201 cgaaagacga gccgcggcta cgcgacgccg ttgcgcagca cgacctcgaa caatcaagtc
     1261 atcggcaagc tttcgacaag ctcccgacat atcgagcaat gatttagcta acggctcaac
     1321 agcatatcca ccgacaacgc gccggcgccg ccgaccgcga tagtcagagc catgagcatc
     1381 aacgccatcg tgtactcgat gccgctgcct tttcccgctg ccgaggaaag aaaaaagcca
     1441 tgctcccagt ggctcaaaaa tgtcgcgaac gccatgaaca ccgccaggcc aaaggccacc
     1501 ggcttgacga agaagccgag caccatgcac acgaccgtcg acagctcgat aatcaatgct
     1561 aaaacaccca tgggcaacgg gataccgtac ttatcgcgcc aattggtcac ttgtgctttt
     1621 ggccccttgc cgccatgcca tccaaacgca tgctgcgtcc catgcataaa aaaggtcacc
     1681 gccaagccga aacgaagaaa aagaaatgcg tacgtatcgt aggtttgaaa aatccagtcc
     1741 actgcctgct cccccttcta ttcttgataa gctatgaaca ttgccacacc aatattgacg
     1801 ccagcgtctc ggcgaacata gcagatgccg ggaaaagatt gcaaagtatt gggtaaacgg
     1861 catagatttc gccctaagct ttatccacta aggagttgaa ccgaatgcaa atcatcgatg
     1921 ccgacggcca tatcaacgac catatctgcg gcgaagagat cgccaagtat atgccgaagg
     1981 gcaatcagat ggcgaaactg tttcccgagt tcgatcatct gcactttcgc tacttgaaag
     2041 aaaatcgccg cgccacgggc aaccccgacg cggcggcgtg gagcgagttt ctcgaaatga
     2101 ccggcatcag ttggaccgtg ctctacccca ctgcgggcct cgccgtcggc cgcatcatgg
     2161 cgcccgactg ggcggtgatc gcttgccaag cttacaatag ctggctccat gaaaagtttt
     2221 tgaccaagaa cccgcgcctc aaaggcgtcg cgctgattcc gttccaggat gtcgacgcgg
     2281 cgattgtcga attgcgccgc gcagtcaaag aacttggcat gttgggcggc atgctgccgt
     2341 ccaacggcga ggcgattcaa ggccatttcg gcaataagat ttactggccg ctctatgcag
     2401 aagcggaaaa attggactgc tgtcttgccg tccacgtcgg cgcgcttcat cacatgggca
     2461 tggatgcatt cacgacttac tatccggtgc acgccttggg ccatccattt agcttgatga
     2521 tccaagcagc tgctatgatc ggtcacggag tattcgatcg ctttacccaa ctgcgcgtcg
     2581 gctttctcga aggcggcgcc acctgggtgc cgttcatgat ggaccgtctc gaccgctcct
     2641 atcacgacgg ccacgtgcaa acggacgtcc atggcaaccg cctcggtggc ccggcgccag
     2701 gcgaaaaagc cagtgattat ttcaataccc aactgcgcga ggggcgcatc tttgtcggct
     2761 tcgattgcga cgatgacgga ttgaataccg cgatcgccaa aggcgggcgc caatcatttt
     2821 tgttcggcag cgacttcccc catgaagtct tcgacgccgg caaatgccgc cacgagatcg
     2881 acgagttgct cgcccgcgac gacctaaccc aagacgacaa agaagccgtg ctcggcggca
     2941 acgccatgag cttttatcgg ctgggaatgt aacaccctgt tatcgggaaa ctaaccgcag
     3001 ggcgggaatg cgagaaaaac accgtgtcgc ggaaggtctc cgcgtttccg cggcaaagcc
     3061 cactgccatg ctcgctccgc cagtgttatg ctaggcggac aaaagcaact aacgtcagga
     3121 atcaccacgc cgtgttcgaa ccatgcgctt catgggacac cggcgcgagg acgcgatacg
     3181 gatcgccaaa ctcggcggcg acttcgagca cctcgggatg gtgcaacagc
//
LOCUS       DASQKS010000232         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4317277, whole genome shotgun sequence.
ACCESSION   DASQKS010000232 DASQKS010000000
VERSION     DASQKS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4317277"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VE170_06040"
     CDS             complement(<1..266)
                     /locus_tag="VE170_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15032.1"
                     /translation="MRISNGALGHIVLNVADLKKSEQFYRTVLGLKVSARNLQTKMTF
                     MSYGREHHDIALQALPKGAKLVRGNGVAKLHHFCFYVDKNEIID"
     gene            403..1110
                     /locus_tag="VE170_06045"
     CDS             403..1110
                     /locus_tag="VE170_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYJ15033.1"
                     /translation="MKIMVVMGEYPAEEGERRRQAVLKCASPGTEIGFDVIKATFFRH
                     SNSQVNSLSAGPLVAEIAIRAEANGYDAVVPFGTLDAGVELSRNLVKIPIVGAGQSVL
                     HLGAQLSNRLGVIAYEDKSVPFMRKQMHVWRVADFVVGIRGIGIPLPESTKNRDAMRS
                     HFIEVARYLIDQCDAEVIVPMGVTMVPVQYSPQEMEKELGVPVMDALKTSIQTAEMMV
                     RMGLTHSVRTYPRPAVQ"
     gene            1150..2019
                     /locus_tag="VE170_06050"
     CDS             1150..2019
                     /locus_tag="VE170_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYJ15034.1"
                     /translation="MRRFDLVEPRSMSEACALLAHDSDAKAVAGGTALLTIIKQGLLL
                     PKLLVNLKKIHDGSAITFDPVQGLRIGALATINEIETSQIVIRNYRALAEACHVVANI
                     RIRNMATIGGNLAHGDYQSDPPTVLAALDARLELLSAGKVRQLALTDFQLGSYETVLQ
                     PGELLAAVVIPPLPPGMNGNYVKFTTGSSGERPCAGVAALARFERGICHELRLAVGAV
                     SAKPLRIAAAELATGKALSVELIATIAAAAARTIDPIDDVRGPAEYKRHLVGVMTRRA
                     LQTLANGQSERKS"
     gene            2016..>2541
                     /locus_tag="VE170_06055"
     CDS             2016..>2541
                     /locus_tag="VE170_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ15035.1"
                     /translation="MTLVGTNVEMVGGRAKVSGAVSYVADMEFAGQLYAKALRSPYPH
                     AKLLRIDASKAAVLPGVRAVVTRDDLAGLNPYFGTGVEDQPVLVIDKVRCVGDIVAAV
                     AADSREIAEEAVALIEADYQELPAATDILEAAKADAPIIHDLHVDKSAGGNIHGVYRA
                     SSGDIEQGFKESDEV"
BASE COUNT          536 a          722 c          769 g          514 t
ORIGIN      
        1 tggtcgatga tttcgttctt gtcgacgtag aaacagaaat gatgcagctt ggccacgcca
       61 ttgccgcgca caagtttggc acccttgggt aatgcttgca gcgcgatatc gtgatgctcg
      121 cggccgtagc tcatgaaggt cattttggtt tgtaagttgc gcgccgaaac cttgaggccg
      181 agaaccgtcc ggtagaattg ctcggacttc ttcagatcgg cgacattgag gacgatgtga
      241 ccgagggcgc cgttggagat tctcatagga agcctcaatt aatcttgctc tgaaggagct
      301 gtcaaggcga tccaagccgt aaatcgtccc gacggtttga caagtcttta aggaatacgg
      361 gatattcgcc gcagtcattt gcgcgccaat gaggagaatt cgatgaaaat catggtggtc
      421 atgggcgagt atccagccga agaaggcgaa cggcggcgcc aagcggtgct caaatgcgcc
      481 tcgcctggaa ccgagatcgg ctttgacgtg atcaaagcga ctttctttcg ccattccaac
      541 tcgcaggtca attcgctgtc ggctggtccg ttagtcgccg agatcgccat ccgcgccgaa
      601 gccaacggtt acgacgctgt ggtgcccttc ggtaccttgg acgccggcgt cgagctttcg
      661 cgcaacctgg tcaagatccc catagtcggc gccggccaat cggtgctgca tctaggcgcg
      721 cagttgagca accgtctcgg cgtgatcgcc tacgaagaca agagcgtccc ctttatgcgc
      781 aaacagatgc atgtctggcg cgtcgccgac tttgtcgtcg gcatccgtgg catcggcatc
      841 ccgctgccgg agagcaccaa gaatcgcgac gccatgcgca gccacttcat cgaggtcgcc
      901 cgctatttga ttgaccagtg cgacgccgaa gtcatcgttc ccatgggcgt gaccatggtg
      961 ccggtgcaat attcgccgca ggagatggaa aaagaactcg gcgtgccggt gatggacgcg
     1021 ctcaagacca gcatccagac agcggaaatg atggtgcgca tgggcttgac ccatagtgtc
     1081 aggacttatc cgcggccggc ggtgcaatga gtttgagaat gagcatatag agggcggaaa
     1141 tggttttcga tgcgtaggtt tgacttggtt gagccgaggt cgatgtcgga ggcttgcgca
     1201 ttactggccc acgacagcga tgccaaagcg gttgccggcg gcacggcgct gctgacgatc
     1261 atcaagcaag gtctgttgct acccaagttg ctagtcaact taaaaaaaat ccacgacggc
     1321 tcggcaatta ccttcgatcc agtccaaggc ctgcgtattg gcgcgctggc gacgatcaac
     1381 gagatcgaaa cctctcaaat cgtcatacgg aactatcgcg cactcgccga agcctgtcat
     1441 gtggtggcga acatccgcat ccgcaacatg gccaccatcg gcggcaatct cgcccatggc
     1501 gactatcagt ccgatccgcc gaccgtattg gccgctttag atgctcgcct cgaactcttg
     1561 agcgcgggca aggttcgcca gcttgcgctg acggattttc aactcggcag ttatgaaacc
     1621 gtgcttcagc cgggagagct gcttgccgcc gtggtgattc cgccgctgcc gccgggaatg
     1681 aacggcaatt atgtcaaatt caccaccgga tcttccggag agcggccgtg cgccggcgtt
     1741 gccgcgttgg caagatttga aagagggatt tgccatgaac tgcgcctggc ggtgggcgca
     1801 gtgtcggcta agccattgcg tatcgctgcg gcagagcttg ccaccggcaa ggcgctaagc
     1861 gttgagctta ttgccaccat tgccgccgct gcggcacgta ccatcgatcc catcgacgat
     1921 gtgcgcgggc cggcggaata caaacgtcat ctggtcggcg tgatgacccg ccgcgcgctg
     1981 caaactctag ctaatggtca gtcggagaga aaatcatgac cctggtcggc accaacgttg
     2041 aaatggtcgg cggccgggcc aaggttagcg gcgcggtgag ctacgtcgcc gatatggagt
     2101 tcgccggcca gctctacgcc aaggcgctgc gcagccccta tcctcatgcc aaactactgc
     2161 gcatcgatgc cagcaaggca gcagtgctgc ccggtgtgcg cgccgtggtg acacgcgacg
     2221 acctggccgg attgaatccg tactttggca ccggtgtcga agatcagccg gtgttggtca
     2281 tcgacaaagt gcgctgtgtc ggtgacatag ttgccgcggt ggccgccgat agccgggaga
     2341 tcgccgaaga ggcagtggcg ctgatcgaag ccgattacca ggaactgccg gcagccaccg
     2401 acatcctcga agcagcgaag gcagacgcac cgattatcca cgatctgcat gtcgacaaat
     2461 ccgccggcgg caacattcac ggcgtctacc gcgcctctag cggcgatatc gagcagggct
     2521 tcaaggagtc cgatgaagtt a
//
LOCUS       DASQKS010000233         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4317337, whole genome shotgun sequence.
ACCESSION   DASQKS010000233 DASQKS010000000
VERSION     DASQKS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4317337"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..522
                     /locus_tag="VE170_06060"
     CDS             100..522
                     /locus_tag="VE170_06060"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HYJ15036.1"
                     /translation="MKPSKPRILQYELPGGWTLWVGGTDADNDYLSTQIAKPDDWWFH
                     ADGVPGSHVILRAKADAEPGRDTLRQAAAVAAYHSKARNGGTTGVHCTRARYVRKPRG
                     VKTGTVQVARGDTLRVRPDISAALRVPTLLGVDEDADP"
     gene            691..1956
                     /locus_tag="VE170_06065"
     CDS             691..1956
                     /locus_tag="VE170_06065"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15037.1"
                     /translation="MIAAEENQLLTQTDPGSPCGAMMRRYWQPVALSEELPAGAAPLK
                     VKILGEELALFRDDQGRPGLIGMHCSHRGTDLSYGRVEDGGLRCLYHGWLYDICGRVL
                     DQPGEPGGGANKSAIRHLAYSCQEVGGVIFTYMGPGEPPLLPNYEFLTVAPQYRTVVK
                     AFYECNYLQGNEGNIDPVHLSFLHQYLSEAEVARLRVVESINATDNTLLAKDVAPTIE
                     VEVTHFGLRIYTTRDASADKRYLRVTNFIMPNLSAFGGSTVGDGYAVHWHVPIDDRHH
                     WKYIFAFSRTSPLDDFLRTRSRVELSADYRLGKTGGNRYQQDRASMKTHTFTGMGTNF
                     QVHDAFATESQGAVQNRTEENLVSSDKAIVAARKLILNAIKDVQQGREPQHVIRDAKA
                     NAFPNLVVISDVIAKSMDWKEYTRSRELK"
     gene            2002..3000
                     /locus_tag="VE170_06070"
     CDS             2002..3000
                     /locus_tag="VE170_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15038.1"
                     /translation="MRHLTAKPVRLVGAICFFLLTAASAAVAQERYLIAYAGFSGFQS
                     PLWATKELGLLAKYGINADLILTPGSTRQIQAMLGNSVHFAQVDAVTTINAIAQGADL
                     VMISGSLNTFPFSFVAQKEIRKPEDLTGKKVGIVGFGGANELAVLLALKEWNMPRNAV
                     TILQAGGAPQRYLALVTKALDATVLAQPEIGEAQRMGMNVLVHMRDMKTAAFPMNVMV
                     VRRSFLQQNRELVKRYQQAYAEATYQLLNSKEKATAVLSKWLQQKNPKAIDETYQYIA
                     TSFSFPTRIAAQGIRNTLDMVAEKNPKIDKNPAKYIDESTLDELEKEGFFKRITTK"
     gene            3303..3379
                     /locus_tag="VE170_06075"
     tRNA            3303..3379
                     /locus_tag="VE170_06075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3337..3339,aa:Val,seq:cac)
     gene            3713..4717
                     /locus_tag="VE170_06080"
     CDS             3713..4717
                     /locus_tag="VE170_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ15039.1"
                     /translation="MANVIPRPETITKLRYAADSALAMLAGMQLDVFTPLKEGPKTIE
                     DIASDLGINSSRLHLLMYSLVDGGLLTEDQGLFSNTPEAHQFLVKGSPAYIGDWHSTF
                     SNRWIRNLKIAESIRSGKPVAKLDFSNSPEEELEKYFRRINLLTVAAAHSLLERYDFS
                     STKTLADVGCGAAGIALTVTNACPHIQATAIDLPQVVPITQKIIDSEGATQRIRVLTA
                     DVLSGSLPGAYDTVVLRAVLQVLSSEDARLAVKNVAPAVRRGGTIYIIGHILDDTRTS
                     PPEALGFNLTSISQFDSGESYTEQEHRSWLSEAGFTDIECSNFMLADEHRVMIARQPA
                     "
     gene            4804..5652
                     /locus_tag="VE170_06085"
     CDS             4804..5652
                     /locus_tag="VE170_06085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ15040.1"
                     /translation="MADNSRDFTAIYQTDDRADFWAREKLRRQESSGAATETLIKAAD
                     LHIDDRVLEVAAGTGDLAVTVAQAVGSSGHVVAVDSSPQMLAHAGNAAREAGLNNIET
                     RVMNAEALEFEDNSFNAVICRMGLMLFENPLTALSEMHRVVKLGGKVAVMVWSSADRN
                     PFHGLPLQIARRIGNIPAPGPDKPGLFALGDSERLEDLFRKVGFRNGSSQSSTFHRKY
                     VSLQDAITLIKSSGGAAALQNVMVNLSLPDQDKAWVEITEALIQFDGPNGFEAPGEVL
                     IATGTK"
BASE COUNT         1379 a         1543 c         1649 g         1291 t
ORIGIN      
        1 gcggcggagt tcctcgatat gtctttgctg aaaagcatgg aggacgaggg gttcttcaag
       61 aagctctatg ggaaatagcc gctggcgggg ccgccgattt tgaaaccatc taaaccgcgc
      121 attcttcagt atgagctccc cggcggctgg actctgtggg tgggcggcac cgacgccgat
      181 aatgattatc tcagcacgca gatcgcgaaa cccgatgact ggtggtttca tgccgacggc
      241 gttccgggca gccatgtgat tttgcgcgcg aaagcggacg ccgagccggg gcgggatacg
      301 ctacgacaag cagccgctgt cgcggcctat cacagcaagg cgcgcaacgg cggcaccact
      361 ggcgttcact gcacgcgagc gcgttatgtg agaaagccgc gcggcgtcaa gaccggcacg
      421 gtgcaagtgg cgcggggcga tacactgcgg gtgcgccccg atataagcgc tgcgctgcga
      481 gtgccgacgc tgttaggcgt cgacgaagac gcggacccgt agtcatccca gatcatgcaa
      541 agattagccc tcgcgtactg cgacggcgat ggcaggatca tcgcggagtt ggcaatgcga
      601 cttcgggaat aagccccgct tgcccaagca cggggcaaat agtaataacc actggtatgc
      661 atgatccaat tcgtgtggag gtgatgccgc atgattgccg ctgaagagaa ccagctgctg
      721 acccaaaccg atcccggcag cccctgcggc gcgatgatgc ggcgctattg gcagccggtc
      781 gcattgagcg aagaattgcc cgccggcgcg gcgccgctca aagtgaagat tcttggcgaa
      841 gagttggctc tattccgcga cgatcaaggt cggccgggct tgatcggcat gcactgctcc
      901 caccgtggca ccgacttgag ttacggccgt gtcgaagacg gcggattgcg ctgtctctac
      961 catggttggc tttacgatat ctgcggccgg gtcctcgatc agccggggga gcccggcggc
     1021 ggcgcgaaca agagcgcgat tcgtcacttg gcctattcct gccaagaagt tggcggtgtg
     1081 atatttacct acatggggcc gggggaaccg ccgttgttgc ccaactacga gtttctcacc
     1141 gtcgcgccgc agtatcgcac cgtggtcaag gcattctacg aatgcaacta tctgcaaggc
     1201 aacgaaggca atatcgatcc ggtgcatctg tcgttcttgc accaatattt gagcgaggcg
     1261 gaagtggcgc gcctgcgcgt cgtggagagc atcaatgcca cggataatac gttgctggcc
     1321 aaagacgtgg cgccgaccat cgaagtcgag gtgacccatt ttggtctgcg aatctatacg
     1381 accagggatg ccagcgccga taagcgttac ttgcgcgtga ccaatttcat tatgccgaat
     1441 ttgtccgcct tcggcggctc caccgtgggc gacggctatg cggtgcattg gcatgtgccg
     1501 atcgacgacc gccatcactg gaaatatatt tttgcattta gccgcaccag tccattggat
     1561 gactttctgc gcacccgcag ccgggtggag ctgagcgccg actatcgcct gggcaaaact
     1621 ggcggcaatc gctatcaaca ggaccgcgcg tcgatgaaga cgcacacctt caccggcatg
     1681 ggcaccaatt ttcaagtcca tgacgcattt gcgaccgaga gtcagggcgc ggtgcagaat
     1741 cgcacggagg agaacctggt ctcgtcggac aaggcgatcg tggcagcgcg caagctgatt
     1801 ttgaacgcca tcaaagacgt gcagcaaggt cgcgagccgc agcatgtgat tcgcgacgcc
     1861 aaggccaatg cttttcccaa cctcgtggtg atttccgatg tgatcgcgaa atcgatggat
     1921 tggaaagagt acacaagaag ccgcgaacta aaataattct aaaataacaa tctctgccaa
     1981 agacgccgag gtgacgacac aatgagacat ttaacggcta agccggtacg gctagtgggc
     2041 gcgatttgct tcttcctgtt gacggctgcc tcggctgcgg tggcgcagga gcgctacctg
     2101 attgcctatg ccggcttctc cggttttcag tcgccgttgt gggcaaccaa ggagttggga
     2161 ctgctcgcca agtacggcat caacgccgat ttgattttaa ctcccggttc gacgcggcag
     2221 atccaggcca tgctcggcaa tagtgtgcac ttcgcccaag tcgatgcggt gaccactatc
     2281 aacgccatcg cccagggcgc cgatctggtg atgatctcgg gttcgctcaa tacgtttccg
     2341 ttcagcttcg tcgcccagaa ggagattcgc aagcccgaag atcttaccgg caagaaagtc
     2401 ggcatcgtcg gcttcggcgg cgccaacgag ttggcagtac tcctggcgct caaagagtgg
     2461 aacatgccgc gcaatgcggt gacgatcctc caagccggcg gcgcgccgca gcgctacttg
     2521 gcgctggtca ccaaggcgct cgacgccacg gttctcgccc agccggaaat cggcgaagcc
     2581 cagcgcatgg gcatgaacgt gcttgtgcac atgcgcgaca tgaagaccgc ggcgtttccg
     2641 atgaacgtca tggttgtgcg ccgctcgttc ttgcaacaaa atcgcgagct ggtgaagcgc
     2701 taccagcagg cctacgccga agcgacctac caactgctca acagcaagga aaaggccacg
     2761 gcggtgctgt ccaagtggct ccagcagaaa aatcccaaag cgatcgacga aacctatcag
     2821 tatatcgcca ccagtttttc tttcccgacg cgcatcgcag cccaaggcat tcgtaacaca
     2881 ctggacatgg tggcggaaaa aaatcccaag atcgataaga atccggcgaa gtatatcgac
     2941 gaaagcaccc tcgatgaatt agagaaagag ggattcttca agcgaatcac tacgaagtga
     3001 ttcgtcaagg cttaaaaggt caaggttaag ggtgttcgga ccgggctttg ctaaatactc
     3061 ctgccgagga gcagtgtttt caccacagcg gtggttggtg tcaggcgtgt cagcaagccg
     3121 cggcgttcgt gaacatcgcc gctaacttgg catgaatcaa gatgaagaag tttatcaacc
     3181 ttggaattcg gaatggctcg gtggctaatc gttgcggctg ataacgtccc taatatatta
     3241 ttgctgacaa gccggtcgca gttgaagctt tcggttgcag gtgttagaaa agatttccca
     3301 gtggtcgcat agctcagttg gttagagcgc gcgcctcaca tgcgcgaggt cgtaggttca
     3361 agtcctactg cgaccaccat ttcctttcct tttcagttag ttattccaca caaaacaaat
     3421 gttccgacgc atcggtgact gtaatgtgac cgagggccga ggtttgtcat gatgagaaag
     3481 aatggcttag gcacagttta tcaacggggt cagtcctggg tgattgatta ctatatcaat
     3541 gggaagatct gagagccaag ccagggctga gctaaaagag agaatcagcc agatccacac
     3601 tagcacgttc atccgctatg aagaaaagct tggctgctca acttcctgcc aagccagggc
     3661 ttgacaattc ttcccgaact gtactggagt ccgatacgcg gaggctaagc ctatggcgaa
     3721 cgtaattcct cgccccgaga ctattaccaa attgaggtac gctgccgact cagcccttgc
     3781 gatgctggct ggtatgcagc ttgatgtgtt cacgcctttg aaggaaggtc ctaaaacaat
     3841 tgaagatata gcttctgacc tggggatcaa ttcttcacga cttcatctgc tgatgtattc
     3901 gttagtggac ggagggctgc taactgaaga ccagggactc ttctctaata ctcccgaagc
     3961 gcatcagttt cttgtgaagg gtagtccggc ttatataggt gattggcatt caactttttc
     4021 caaccgctgg attcgtaatc tcaagatcgc cgaatcgatc cgttccggga agccagtagc
     4081 aaaactcgac ttttccaatt ctccagagga ggaactagag aaatactttc gtcgaattaa
     4141 cctgcttacc gtggcagcgg cccattctct actggagaga tatgactttt cctcgacgaa
     4201 gacattagcg gatgtaggtt gtggagcggc gggaattgcc ctgacggtta caaacgcatg
     4261 tccgcatata caggctactg cgatcgatct gcctcaggtc gtgccgatta ctcaaaaaat
     4321 catcgattcc gaaggcgcga ctcagcggat ccgcgttttg accgcagatg tactaagtgg
     4381 atctttgccc ggcgcttatg acacggtggt tttaagagcg gttctgcaag ttttgtcttc
     4441 ggaagatgct cgccttgcag tgaaaaatgt agctcccgca gtacgacgcg gcggaacaat
     4501 ttatatcatc gggcatattc ttgatgacac acgaacgtcg ccgccagagg cattgggctt
     4561 caacctgact tcgatcagtc aatttgactc tggtgagtcc tatacagagc aagaacacag
     4621 aagttggctc agcgaggcag gatttacgga cattgagtgt tcgaatttca tgttagcgga
     4681 tgaacatcgt gtcatgatcg ctcgacaacc ggcatagaca ttactcgatt acatttcggc
     4741 tgacagttga cctcgcgttc ttatcgacag tagcggttgg ataagcctaa tggaggaaat
     4801 tttatggctg acaattcacg ggatttcacc gcaatctacc aaactgatga tcgagcagat
     4861 ttttgggcgc gagagaaact caggcggcag gagagttcag gcgcggcaac ggaaacgcta
     4921 ataaaggctg cggatctcca tatcgacgac cgcgtcttgg aagtcgccgc tggaacagga
     4981 gatctggctg taaccgtcgc tcaagccgtt ggttccagcg gtcatgttgt tgcggtagat
     5041 agttcgcccc aaatgttagc tcacgcggga aacgcagctc gagaggcggg ccttaacaac
     5101 attgaaactc gtgtcatgaa tgctgaggca ctcgaattcg aagacaattc attcaacgcg
     5161 gtcatttgca ggatgggact gatgttgttt gagaacccgc ttaccgcact cagcgagatg
     5221 cacagagtcg taaagctcgg gggcaaagtc gccgtgatgg tgtggtcgtc agccgaccga
     5281 aacccgtttc atggactgcc tcttcagatc gctcgccgta tcggaaatat ccccgctccc
     5341 ggaccggata aacccggctt gtttgcgctc ggcgatagcg agcggctgga agatttgttt
     5401 cgaaaggtcg ggtttcgcaa tggatcaagc caatcatcaa cctttcatag gaagtatgtc
     5461 tctcttcagg acgcgatcac tttaataaaa agctctggag gcgctgccgc attgcaaaac
     5521 gtgatggtca atctgagcct ccccgaccaa gacaaagcct gggtggaaat caccgaagca
     5581 ttaattcagt tcgatggacc gaatggattc gaggctccgg gtgaagtgct cattgcgacg
     5641 ggaaccaaat agaacttccg cgagaagtct tcaacaagtt cgacctactt cccctcttag
     5701 ctgaagagct gatacgtctc gatgttgaca tgttcagtcg agcagccgag gtctgaaatg
     5761 gtcctctgct cgcttacatg aaaggtaaca ctccacgcac cgcccaaact ttcactttaa
     5821 ttgtgaatgg tctgtcgggg ccgtttccca acacgttctt ac
//
LOCUS       DASQKS010000234         4436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2223569, whole genome shotgun sequence.
ACCESSION   DASQKS010000234 DASQKS010000000
VERSION     DASQKS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223569"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VE170_06090"
     CDS             <1..1033
                     /locus_tag="VE170_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15041.1"
                     /translation="DLDVKRGEFFVLLGPSGSGKTTLLRSVAGLEKPDSGEILLDDKL
                     VFSSSRRISEPPEARGIGMVFQSYAIWPHLTVAENIGLVLTHGRRRLSKNEAQTRIAY
                     ALSLVQLDDFENRPARLLSGGQQQRVALARALAVNPALLLMDEPLSNLDARLREEVRT
                     NIKKLATRLGITALYVTHDQVEAMVLADRIAVMAHGEILQIGNPFELYRAPANSQIAE
                     FFGSINWLHGKVLADGRIDSEIGPLVIAAARQYEGAVVVGIRPEDVKLIAQPGAAENC
                     PAGNVVGSFFLGDHVMSEVMIGETIINVKSLPDDADAKVTGTVTLHLPKQRMVIFPAT
                     ESIQSLQAK"
     gene            1055..1882
                     /locus_tag="VE170_06095"
     CDS             1055..1882
                     /locus_tag="VE170_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15042.1"
                     /translation="MSGNAKANGDVHHLDHFVVAVMEPDRAEKFYTEVLGARTLKTQN
                     APNMTRIFMKLGENHVGLFSQGKNQLPKRDSANSYPRHSFEVPAGEYDSMVAKIRGAG
                     VFTSEIKLESALGCCWNQGLVFQDSEGNFFEITKASGIAKTRLHHLHFDSTDIDASLD
                     FYKKFLNCTVGTRHDAMAEIVFPTGQSVIVNKVDDLSDVTKATWRGRHFALNVSNDNF
                     HAIVAKLHAAGIEERDDHGEREGRRDDQLGTYFKEPSGFRLQITNEDSATFAAHAND"
     gene            2209..2997
                     /locus_tag="VE170_06100"
     CDS             2209..2997
                     /locus_tag="VE170_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15043.1"
                     /translation="MPYSIRNGVKSYYEVSGEGYPLVLMHANPFDRRMFLYQVAHFST
                     FMKVINIDLRAYGYSDKPATPVTMTDLCEDVVAVCRQEGANQAVFAGVSVGGVMGLQL
                     GLDHPELFKALILVGCSSMPGDRYQSRIDGYVGQGVGTFHIQHLTDLVSKDFPQTKLG
                     KYLLSMHTEMDSRLNAPAIAEIFNALQHRDLTARLPELKMPVLIMNGEFDNSLKRSKE
                     MSTRIAGAEHRTIQGAGHACCLEDPATFDSHVLDFLKKHGILKA"
     gene            3032..4036
                     /locus_tag="VE170_06105"
     CDS             3032..4036
                     /locus_tag="VE170_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15044.1"
                     /translation="MRNMFGQIIQMMFLVFGLGFSFAYGSEWKDKAEAEGKLTWYASL
                     GATDARRIIDRFKELYPKIDAQFYRAGDATLMERILAESRAGKFEWDVVSTTGFYAHN
                     LKKRGMLAAYDSPERKFIRAGHKDPQAMWTSIYTNYTVLGYNSRLVSRDNVPKSHADL
                     LKPLWRGQVGIVQTAYEWFAVMLQAMGQEKGMAFMRELAKQQPQLRNGRTLLAQLVAA
                     GEVNSALAAYSQNFESLKRDGAPVDWVPLDPVYANLNPMGLSAKSPRSNAGKLFIDFV
                     LSKAGQETIRAQRRVPDRIDVLPDPPKLAQGFTAVFTAEQVYENFDRYVKLFQDTFGK
                     "
     gene            complement(4089..>4436)
                     /locus_tag="VE170_06110"
     CDS             complement(4089..>4436)
                     /locus_tag="VE170_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15045.1"
                     /translation="SARKRDPRLPETPTIYELMDQYKTAEQSRRVATVILAGGVFGRP
                     LVGPAGIPPETVKTLRAGVASALKDGELKAEAEKRNYELDPVGGEEMQRLAKEVMSQP
                     PAVLERMKKVLGE"
BASE COUNT          961 a         1205 c         1323 g          947 t
ORIGIN      
        1 tgacctggac gtgaagcgcg gcgagttctt cgtgctgctc gggccgagcg gatcggggaa
       61 gacgacgctg ttgcgcagcg tcgccgggtt ggagaaaccc gatagcggcg agattttact
      121 cgatgacaag ctggtcttct ccagcagccg gcggatttcg gaaccgccgg aagcgcgcgg
      181 catcggcatg gtgtttcagt cctatgcgat atggccccac ctcaccgttg ccgaaaatat
      241 cggcttggtg ctgacccatg gccgcaggcg tctgtccaaa aacgaggcgc aaacgcgaat
      301 cgcttacgcg ctcagcttgg tgcaattgga cgatttcgaa aaccgcccgg cgcgcctgtt
      361 gagcggcggg caacagcaac gggtcgcgct ggcgcgtgct ttggcggtca atccggcctt
      421 gctgctgatg gacgagccgc tgagcaattt ggacgcgcgt ttgcgcgaag aggtgcgcac
      481 aaatatcaag aaactcgcca cccgattggg cattaccgcg ctctatgtca cccatgatca
      541 agtcgaagcg atggtgctcg ccgaccgcat cgcggtgatg gcccatggcg aaattttaca
      601 aatcggcaac ccgttcgagc tctaccgcgc accggctaat tcgcagatcg ccgagttttt
      661 cggttcgatc aactggctgc acggcaaggt gcttgccgac ggccgcatcg acagcgagat
      721 cggcccgctt gtcatcgctg cggcgcggca gtatgaaggg gctgtagtcg tcggtattcg
      781 gcccgaagat gttaaattga tcgcgcagcc gggtgcggcg gagaattgtc cggcggggaa
      841 tgtggtcggg tcgttttttc ttggcgatca tgtcatgagc gaggttatga tcggcgagac
      901 gattatcaac gtcaagagtt tgcctgacga cgccgatgcc aaggtgaccg gcactgtgac
      961 gctccatttg ccgaagcagc gcatggtgat ttttcccgcg acggagtcga tccagtcgtt
     1021 gcaagcaaaa taagcgtgag aaggagggaa agttatgagc ggcaatgcaa aagccaatgg
     1081 cgatgtccat catctcgatc acttcgtggt cgcggtgatg gagccggacc gggccgaaaa
     1141 attctacacc gaggtgctcg gcgcgcgcac tttgaaaacc cagaacgcgc cgaatatgac
     1201 gcgtattttc atgaaacttg gcgagaatca tgtcgggctg ttttcccaag gaaagaacca
     1261 gttgcccaag cgcgacagcg ccaattccta tccgcgccat tcgttcgaag tgccggcggg
     1321 cgagtacgac tcgatggtcg cgaagattcg cggcgccggc gtattcacga gcgagatcaa
     1381 attggaaagc gctttgggtt gttgttggaa ccaagggttg gtcttccaag attccgaagg
     1441 caacttcttc gagattacca aagccagcgg catcgccaag acgcgcttgc accatttgca
     1501 ttttgacagc accgatatcg acgccagtct ggatttctac aagaaatttc tgaactgcac
     1561 ggtgggaacg cggcatgatg ccatggcgga aatcgttttt ccgacggggc aaagcgtgat
     1621 tgtgaacaaa gtcgatgatc tgagtgacgt gaccaaggcg acctggcgcg gccgccactt
     1681 tgccctcaac gtgagcaacg acaactttca cgccatcgtc gcaaaactcc atgcggcggg
     1741 aatcgaagaa cgcgacgatc acggcgagcg cgaaggccgg cgcgacgatc agttggggac
     1801 ctatttcaaa gagcccagcg gctttcgcct gcaaatcacc aacgaagact cggcgacgtt
     1861 tgcggcccac gccaacgatt gagcagccct ttcatcacga ggacacgaag agcacgaagt
     1921 tcggaaatta tatatccgaa gccttcgtgt ccttcggctt ctttgaaaat acatgtgagg
     1981 ggatgatatg gacaacgcga aggcgtcaag agctccgtca tactcgaaag cgggtatcca
     2041 ggctaattcg gcgcagacca aacctggatt cccgcatgcg cgggaatgac gaatcgagga
     2101 tgggcgcatg ttctgcgaat gcgaaccatt ttcaggcgcc gtaggcgagc ggaagctcat
     2161 ggacacttca tggtgcgttc cgtttccctg taatctccgg aggtccgcat gccttatagc
     2221 attagaaacg gtgttaagag ctactacgaa gtctccggcg aaggttatcc gctggtgctg
     2281 atgcacgcca atccattcga ccggcgcatg tttctttatc aggtggcgca cttttcgacg
     2341 ttcatgaaag tcatcaacat cgatctgcgc gcgtacggtt actccgacaa gccggcgacg
     2401 ccggtgacca tgaccgatct gtgcgaggac gtggttgcgg tgtgccggca agaaggggcg
     2461 aaccaggcgg tgttcgccgg tgtcagtgtt ggcggcgtga tgggtttaca gctgggcctc
     2521 gatcacccgg agctgtttaa agcgttgatt ctagtcggct gcagcagcat gccgggcgac
     2581 cgttaccaaa gccgcatcga cggttatgtg gggcaaggtg tcggtacatt tcacattcag
     2641 catttgaccg atctggtcag caaagatttt cctcaaacca aattagggaa gtatctgttg
     2701 agcatgcaca ccgaaatgga ttcacgtctc aacgcgccgg ctatcgccga gattttcaac
     2761 gccctgcagc atcgcgactt gacggcgcgc ttgccggagt tgaaaatgcc ggttctgatc
     2821 atgaacggcg agttcgataa ttcgctcaag cgcagcaaag aaatgagcac gcgtatcgcc
     2881 ggcgccgagc atcggaccat ccaaggtgcc ggacatgcat gttgtctgga agatccggcg
     2941 acattcgatt ctcatgtcct ggattttctc aagaagcatg ggattcttaa agcttgatag
     3001 gccgccttag tgcaaccaac gatcagccgc gatgcgtaat atgttcgggc aaatcatcca
     3061 aatgatgttt ttggtcttcg gcctggggtt ttcattcgca tacggttccg agtggaaaga
     3121 caaagccgaa gcggaaggaa aactgacctg gtacgcttct ctcggcgcga ccgatgcgcg
     3181 gcggattatc gatcgcttca aagagcttta tccaaagatc gacgcgcagt tttaccgcgc
     3241 cggcgatgct acattgatgg aacggatcct cgccgaatcg cgggcgggaa agttcgaatg
     3301 ggatgtcgtg agcaccaccg gcttttacgc gcacaatctt aaaaagcgcg gcatgttggc
     3361 ggcgtacgat tcgccggagc gcaagttcat tcgtgccggc cataaagatc cgcaggcgat
     3421 gtggacgtcg atctatacca actacaccgt gctcggttac aacagccgct tggtgagccg
     3481 ggataatgtg ccgaaatcac atgctgactt gctaaagcct ctttggcgcg gccaggtcgg
     3541 catcgtgcag accgcctacg aatggtttgc cgtgatgttg caggcgatgg gacaggaaaa
     3601 aggtatggca ttcatgcgcg agctggcgaa acagcagccg caattgcgca acggccgcac
     3661 gctcctggcg caattggtgg cggcggggga agtcaattcg gcactggcgg cttactcgca
     3721 gaacttcgaa agtctcaagc gcgacggcgc cccggtggat tgggtgccgc tcgatccggt
     3781 ctatgccaac ctgaatccga tgggactctc cgccaagtcg ccgcgctcca atgccggcaa
     3841 gttgtttatc gatttcgtgt tgtcgaaagc cgggcaggaa actattcgcg cgcaacgacg
     3901 cgtgcccgac cgcatcgatg tgctgcccga tccgcccaag ctggcgcaag gctttaccgc
     3961 ggtattcacc gccgaacaag tgtatgagaa tttcgaccgc tacgtaaaac tgtttcagga
     4021 cacgtttggc aaatagtctg cgcagtcgcg atcccgtcgc acttgcttcg cagtgaaatt
     4081 cgtctgagct actcgcccaa cactttcttc attcgctcca acaccgccgg cggttggctc
     4141 atgacttcct tggctaaccg ctgcatttct tcgccgccca ccgggtcgag ttcgtagttg
     4201 cgtttttcag cctcggcttt gagttcgcca tctttaaggg cgctggctac gccggcgcgt
     4261 aaggttttca ccgtttccgg cgggatgccg gctggaccga ccagcgggcg gccgaagacg
     4321 ccgccggcga ggatcacggt ggcgacccgg cgcgattgct cggcagtttt atactgatcc
     4381 atcagttcgt aaatggtcgg cgtttcgggc agacgcgggt cgcgcttgcg cgccga
//
LOCUS       DASQKS010000235         7506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3482209, whole genome shotgun sequence.
ACCESSION   DASQKS010000235 DASQKS010000000
VERSION     DASQKS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482209"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VE170_06115"
     CDS             complement(<1..328)
                     /locus_tag="VE170_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ15046.1"
                     /translation="MDPIKIIIDGHEVVTSKGKTVIEAAAAAGVAIPHYCYHPKLSIA
                     GNCRMCMVEIEKLPKLQIACNTQVTEGMSILTQSPKVVAVRKAVLEFLLINHPVDCPI
                     CDQAGEC"
     gene            complement(411..1679)
                     /gene="nuoF"
                     /locus_tag="VE170_06120"
     CDS             complement(411..1679)
                     /gene="nuoF"
                     /locus_tag="VE170_06120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HYJ15047.1"
                     /translation="MDKLLLRNIGVPGSHTLATYQSRGGYGSWRKVVETLKPEGLIDE
                     VKASGLRGRGGAGFPTGMKWSFVPKDSPKPKYLVCNADESEPGTFKDRLLIEQDPHAL
                     IEGTMIAAFAIQSHTAFIYIRGEMVFGTIRLEQAVAEAYQAGLIGKNIFGTGYDLEII
                     VHRGAGAYICGEETALLSSLEGGRGWPKVKPPFPATHGLFGCPTVVNNVETLAALPWI
                     IEHGAAAYSAIGTEKSKGTKLFSVSGHIVRPGVYEVEMGYPFKKFLEEDCGGVPNGKK
                     LKGVIPGGASMPVLKPDEIEKVNLDYESVAAAGSLLGSGGVIVMDETVCMVRAAWNIA
                     RFYAHESCGQCSPCREGCHWMEKIFYRVEHGEAKKNDLDLILNIAGNIAGNTICPFGD
                     AAAMPAGAFIRKYRDEFEAHIAAGKCTVRG"
     gene            complement(1783..2256)
                     /locus_tag="VE170_06125"
     CDS             complement(1783..2256)
                     /locus_tag="VE170_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HYJ15048.1"
                     /translation="MAVEFSPEALKTFEATIARYPHKEAAMLPVLYLAQREFGHLGAE
                     AIDYVAKLMGQAPARVYSVVSFYTMYNMKPIGRHHIQVCRTLPCALRGAEEVTAFLKK
                     KLGIDCGQTTPDGRFTLAEVECLASCGTAPMMQVNDDYYEDLTEAKIEQILDGMK"
     gene            complement(2340..3530)
                     /gene="nuoD"
                     /locus_tag="VE170_06130"
     CDS             complement(2340..3530)
                     /gene="nuoD"
                     /locus_tag="VE170_06130"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HYJ15049.1"
                     /translation="MKADTPELPHEIMDLQMGPSHPATHGTIKFNLKLEGEKVLDCDV
                     ELGFLHRGFDKSCEQATWTQVIPYTDRLNYASSLINNVGYALAVEKLIGAETPPRCQY
                     IRVIISELARLFDHLTCCGMSASELGAITVGFYMIEARELITRIIENLTGARLTVTYV
                     RIGGLKHDLPENFAEGVTAAFTTIRKLLDDCDRLLSRNRIFLDRMANVGVISKESALD
                     HSLTGPILRATGVDYDVRKAFPYLVYDKLDFAIPVGSRGDNYDRFLCRLGEIQESMKI
                     VEQALRQLPVGPVWIDDRRFVLPEKEKVYGTIEGLMHHFKIIMEGIQVPAGETYSAVE
                     GGNGELGFYIVSDGGGKPYRLRVRPPCFIAMGAFAEMIKGYMVADIVPTFGMINMIGG
                     ECDR"
     gene            complement(3552..4115)
                     /locus_tag="VE170_06135"
     CDS             complement(3552..4115)
                     /locus_tag="VE170_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYJ15050.1"
                     /translation="MAEASPQLQDIQDKLGAKVLSASQPQGDQVVVLDRAGLRESFRL
                     LKEDPELGFNLLSDITAVDYWQKQEPRFEVVYQLVSLQQRRRLRVRVSVPEGDAVVDS
                     LTPLWRGANFLEREVWDLYGIRFSDHPDLRRILLYDEFTGHPLRKDYPVNLCQPRVPE
                     RHVDGTFVDERSRNKLLRLQKTLAKKN"
     gene            complement(4122..4478)
                     /gene="ndhC"
                     /locus_tag="VE170_06140"
     CDS             complement(4122..4478)
                     /gene="ndhC"
                     /locus_tag="VE170_06140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYJ15051.1"
                     /translation="MNHPFLPLLVMFAVAGVIVLALLTVASLFGPKSTNPAKAEPFES
                     GNPPQGDARIRFSVRFYLVAMLFLIFDLEVVFLYPWAIYFRQLGVFGLVQMGIFLVIL
                     TIGYVYVWKKGALEWD"
     gene            4729..5742
                     /locus_tag="VE170_06145"
     CDS             4729..5742
                     /locus_tag="VE170_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15052.1"
                     /translation="MAHRFSTLLSICFTGVLLMNSMAWAQALIIEGAKKEGQVVLYAS
                     MEVPSAQRLVAGFERKYPFLKVEATRIGSERMATRLIAEAQARKVRADVVHQSAFDFY
                     GVLQKGLFDSYLSPERAALPAEYRDDKGLWVMAAATLNVIAYNKKMVSAADAPKSFWD
                     FTAPKWKGQLLMDDNESKWMAGMMQYYGETKTVELLKMLAAQDIQFRTGHSSIQTLLA
                     AGERAAVVVAFANGVDRLKKDGAPIEWIAPEPVIGLTFGTAVVKDAPHPNAARLFNDF
                     LLSREGQEITAAAGYFVPRTDVASPILKETPAKTKIIPLPMTLAPRYNEYFQNYRKLM
                     GLK"
     gene            5837..7198
                     /locus_tag="VE170_06150"
     CDS             5837..7198
                     /locus_tag="VE170_06150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYJ15053.1"
                     /translation="MAYQSEQGNFTIALAGDTMLTRKLTPFAEERFLKLREILHGADA
                     RFANLEGTVHTWDEGTPGITQGTFMTTDPKLLEDLQWLGINLVSCANNHAFDYGEDGV
                     LANLKHLDAAGMVHAGTGKNLAEARAPGYLDTANGRVGLVAATATFRPWNQAGEQRPD
                     LRGRPGINPLGFQTAYYVDAAALEQLQRISRELGFEKSTERARKHFYSDKELASDDSA
                     ELSLLGNRYVLGEGFSIATEVNARDLQDNLRWIREARRQADWVVVSAHCHEFGGSSLL
                     TASTRAELEETADFVTKFAHQAIDEGADIFVGHGSHFPMGVEIYKGKPIFYSVGNFVF
                     QNETVGFFPADAYERFDLDLKATPSDFLDARTNGGKKGHPSDPAYWENMFPVCEFNKQ
                     KLTSIKIYPIDQGFGRPRPQRGRPMLAEGEVAERVIERVVRLSQRYGTKVVNRAGLGL
                     IEL"
BASE COUNT         1678 a         2218 c         2057 g         1553 t
ORIGIN      
        1 ggcactcgcc ggcctggtcg cagatcgggc aatccaccgg gtgattgatc agcagaaatt
       61 ccagcaccgc tttgcgcacc gcgacgacct tgggactctg ggtcaggatc gacatgccct
      121 cggtcacctg ggtattgcag gcgatctgca gtttgggcag tttttcgatc tcgaccatgc
      181 acatgcggca attgccggcg atcgacagct tcgggtgata gcagtaatgg gggatcgcca
      241 ccccggctgc cgccgccgcc tcgatcaccg tcttgccctt gctggtgacg acttcgtgac
      301 cgtcaatgat gatttttatt ggatccataa tgaagattgg agtgatggag tactggagtg
      361 ttggagtatt gcgtccggac ccattactcc atcactccaa tactccattc ctagcctctc
      421 accgtgcatt tccccgctgc gatgtgggct tcgaactcgt cgcggtattt tctgataaac
      481 gctcccgccg gcatcgccgc ggcatcaccg aacggacaaa tcgtattgcc ggcgatattg
      541 ccggcgatat tgagaatcag atctaaatcg ttctttttgg cctcgccatg ctcgacacgg
      601 taaaaaatct tttccatcca atggcacccc tcgcggcagg gactgcattg gccgcaggac
      661 tcgtgggcgt aaaagcgcgc gatgttccat gccgcccgca ccatgcagac agtctcgtcc
      721 atgacgatca cgccgccgga gccgagcaga ctgccggccg ccgccaccga ttcgtaatcg
      781 agatttactt tttcgatctc atcgggcttc aacaccggca tggaagcgcc gccggggatc
      841 acgcctttta atttcttgcc gttgggcacg ccgccgcagt cctcttcgag aaatttcttg
      901 aacggataac ccatctcgac ttcgtagacc cccggccgga caatatggcc gctgacactg
      961 aacagcttgg tccccttgct tttctctgta cctatggccg agtaggccgc ggcgccgtgc
     1021 tcgataatcc atggcaaagc ggctaacgtc tcgacattgt tcaccaccgt cggacagccg
     1081 aacagaccat gcgtagccgg aaacggcggt ttgactttag gccagccgcg gccgccttcg
     1141 agggatgaca gcaaggcggt ctcttcgccg cagatatagg ccccggcacc gcggtgcacg
     1201 atgatctcca agtcgtagcc ggtgccgaag atatttttac cgatcaaacc ggcttggtag
     1261 gcttcggcaa ccgcctgctc caacctgatc gtgccaaata ccatctcgcc gcggatgtaa
     1321 ataaacgccg tatgcgactg aatcgcgaag gcggcgatca tcgtgccctc gataagcgcg
     1381 tgggggtcct gttcgatgag cagtcgatcc ttaaaggttc ccggttcgct ttcgtcggcg
     1441 ttgcagacca aatatttcgg cttggggcta tccttgggta cgaaactcca cttcatgccg
     1501 gtgggaaacc ccgcgccgcc gcggccgcgc aggccggagg ctttgacttc atcgatcagt
     1561 ccttccggct tcaaggtctc gacgaccttg cgccaggagc cgtagccgcc ccgcgattga
     1621 tacgtcgcca gcgtgtgcga gcccggaacg ccgatattgc gcaggagaag tttgtccatg
     1681 aacccttagg agaaattgat aattaacaat agcgactgga gaaagttccg gattcggtca
     1741 ttgtcgattt tccatacttc gtgatcaatt ctgacttcat tgttacttca ttccgtcgag
     1801 aatttgctcg atcttcgctt cggtcaagtc ttcataatag tcgtcattca cttgcatcat
     1861 cggcgccgtg ccgcaagagg ccaaacactc cacttccgcc agcgtgaacc tgccgtctgg
     1921 ggtcgtttgg ccgcagtcga tgccaagttt cttcttgaga aacgcggtga cttcctcggc
     1981 gccgcgcaag gcacagggca aggtgcggca gacctggata tgatggcgcc cgatcggctt
     2041 catattatac atggtgtaaa aactcaccac gctatagacg cgcgccggcg cctggcccat
     2101 cagcttggcg acgtagtcga ttgcctccgc cccgagatgg ccgaactcac gctgggccaa
     2161 gtaaagcacc ggcagcatcg ccgcttcctt atgcggatag cgcgcgatcg tcgcctcaaa
     2221 agttttcagc gcctcaggcg aaaattccac agccattgtt tcaagtcatc gagcatcgac
     2281 tatcgagcat cgcggatccc gccccgtcgc cgtcatagga cgacgcaaat ctcgactctc
     2341 tagcgatcac attccccgcc aatcatattg atcataccga atgtcggcac gatgtccgcc
     2401 accatgtaac ctttgatcat ttccgcaaac gcgcccatgg cgatgaaaca gggcggccgc
     2461 acgcgcaagc gatagggctt gccgccgccg tcgctgacga tgtaaaaacc gagctcgcca
     2521 ttgccgccct ccaccgcgct ataggtctct cccgccggca cctgaatgcc ttccatgatg
     2581 attttgaaat gatgcatcaa gccttcgatg gtgccgtaaa ccttttcctt ttccggcagg
     2641 acgaatcgcc gatcgtcgat ccacaccgga ccgacaggca attgccggag cgcctgctcg
     2701 acgatcttca tactctcctg gatctcaccg agccggcaca ggaagcgatc atagttatcg
     2761 ccgcggctgc ctactgggat tgcaaaatcc aatttatcat acaccaggta gggaaacgcc
     2821 ttgcggacat cgtagtcgac accggtggcg cgcaggatcg gcccggtgag gctgtgatca
     2881 agcgccgatt ccttcgagat cacgccgacg ttggccatac ggtcgagaaa aatccgattg
     2941 cgcgacagta agcggtcgca atcgtcgagc agcttgcgga tggttgtgaa agccgcggtc
     3001 acgccctcgg caaaattctc cggcaggtca tgcttgagcc cgccgatgcg cacataggtc
     3061 accgtcaggc gggcgccggt gagattttcg atgatgcggg taatgagttc gcgggcttcg
     3121 atcatataga aaccgacggt aatagcgccc aactcggaag cggacatacc gcagcaagtc
     3181 aaatgatcga agagccgggc cagctcgctg atgatgacgc gaatatattg gcagcgcggc
     3241 ggcgtttcgg cgccgattag tttctccacg gcgagggcgt agccgacgtt attgatcaac
     3301 gacgaagcgt aattgagccg gtcggtgtaa ggaatgacct gggtccaggt cgcctgttcg
     3361 cagctcttgt cgaagccacg gtgcagaaaa cccaattcga cgtcgcagtc taggactttc
     3421 tcgccttcga gcttgagatt gaacttgatc gtcccatgag tcgccggatg ggacggtccc
     3481 atctgcaaat ccatgatctc atgcggcagc tctggggtat cggctttcat cgagttgttc
     3541 gggcaaagct actagttctt tttcgccaaa gtcttttgca atcgtagcag tttgttacgg
     3601 gaccgctcgt cgacgaaagt gccgtcgacg tggcgttcgg gcactctcgg ctggcacagg
     3661 ttgaccgggt agtccttgcg cagcggatgg ccggtgaatt cgtcgtagag caaaatgcgc
     3721 cgcaaatcgg gatgatcgct aaagcggatg ccatagagat cccaaacttc gcgctcgaga
     3781 aagttggcgc cgcgccacag cggcgtcagc gaatcgacca ccgcgtcgcc ttccggcaca
     3841 ctgacgcgca cgcgcaggcg gcgacgctgc tggagcgaaa ccaattgata aaccacctcg
     3901 aagcgcggct cctgcttttg ccaatagtca acggcggtta tgtcggaaag taaattgaac
     3961 ccaagctcag gatcttcctt gagcaagcga aagctctctc tcaaaccggc gcgatccaga
     4021 acaaccacct ggtctccctg gggttggctg gcactcagaa ctttggcgcc aagcttatct
     4081 tggatatctt ggagttgggg gctcgcttca gccatgaaca atcaatccca ctcgagggcc
     4141 ccttttttcc agacataaac ataaccgatg gtcagaatga ccagaaagat gcccatctgc
     4201 accagaccga agactcccaa ctggcgaaaa tagatcgccc agggataaag aaacacgact
     4261 tccaggtcga aaatcagaaa taacatggcg accaagtaga agcgcaccga gaaacgaata
     4321 cgcgcatcgc cttggggtgg attgccggat tcgaagggtt ctgctttagc gggattggtc
     4381 gatttggggc caaacagcga agcaaccgtc agcaaagcga ggacaatcac gcccgcaacc
     4441 gcgaacatca ccagcaaggg caaaaaagga tggttcatgt catgagtgct atcaaaaaaa
     4501 accatacctc cccggaccga aaaggtatgg attcttacgg gcagtttaat ccttcgatct
     4561 attgaatttt gtattggaaa tgcgcggagt tgtcaaggta aagccagcct cgagggtggg
     4621 ctccacgggg ccaatcttgg gtttgacgag gaacctattg gcgattgacg ttaggcgaga
     4681 ttcgatgtta agcatcacag cgaaatcgcg gttcatcgga ggtttcggat ggcccacaga
     4741 ttttccaccc tgttgtcgat atgtttcacc ggggttctgc tcatgaactc gatggcgtgg
     4801 gcgcaagctc tgatcatcga gggcgccaaa aaagagggtc aggtggtcct ctacgcttcg
     4861 atggaagtac cctcggcgca aagactcgtt gccggtttcg aacgaaaata tccatttttg
     4921 aaggtcgagg ccacacggat cggctcggag cgaatggcca cacgactgat cgccgaagcc
     4981 caagcgcgca aggtccgcgc cgacgtcgtc catcagtcgg cattcgattt ctacggggtt
     5041 ctgcagaagg gtctcttcga tagctacctt tcccctgaac gcgccgcgct tccagccgag
     5101 taccgcgacg acaaggggct atgggtgatg gcagccgcga cgctcaatgt catagcctat
     5161 aacaagaaaa tggttagcgc cgccgacgcg ccgaaaagct tttgggattt tacggcgccg
     5221 aaatggaaag gccagttgct gatggacgac aacgagagca agtggatggc cggaatgatg
     5281 cagtactacg gcgagacgaa aaccgtcgag ctgctaaaaa tgctcgcggc ccaagatatt
     5341 caattccgca ccggccattc ttcgatccaa actctactcg ccgccggcga gcgcgccgcc
     5401 gtggtcgtag ccttcgccaa tggcgtcgac cgtttaaaaa aggatggcgc accgatcgag
     5461 tggatcgcgc cggaaccggt gatcggcttg accttcggca ctgctgtcgt caaggacgca
     5521 ccgcacccca acgccgcccg gctgttcaat gattttcttc tgtctcgcga aggccaagag
     5581 atcaccgccg ccgccggcta cttcgtgccg cgcacggatg tggcttcgcc gatcctcaaa
     5641 gaaacgccgg caaaaaccaa aatcataccc ctgcccatga ccttggcgcc acgctacaac
     5701 gaatatttcc agaactaccg caaactgatg gggctcaaat agaccgacta ggtaccgaga
     5761 ggtcgtgccg agcgaagcca agggcctcgg tttgatcact gccggcaaga acatcatcga
     5821 catttaggag accgagatgg cttaccaatc tgaacaaggc aacttcacca tcgccctcgc
     5881 cggcgatacc atgctcacgc gcaaattaac gcccttcgcc gaggagcgtt ttctcaaact
     5941 gcgcgagatc ctgcacggcg ccgacgcccg cttcgccaat ctcgaaggca cggtgcacac
     6001 ctgggacgaa gggaccccgg ggatcaccca gggcacgttc atgacgacgg accccaaact
     6061 cctcgaagac ttgcaatggc tcggcatcaa tctggtctcc tgcgccaaca atcacgcctt
     6121 cgactatggc gaagacggcg tgctcgccaa cctcaaacat ctcgatgccg ccggcatggt
     6181 tcacgccggc acgggaaaaa acctcgccga agcgcgcgcg cccggatatc tcgacacggc
     6241 caatgggcga gtcggcctgg tcgccgccac ggcgaccttt cggccctgga atcaagccgg
     6301 cgaacagcgg cctgacttgc gcggccggcc gggtatcaat ccgctcggct tccagactgc
     6361 ctattacgtc gacgccgcgg ccttggaaca attgcagcgc atcagccgcg aactcggctt
     6421 tgaaaaaagc accgagcggg cgcgcaaaca tttttatagc gacaaggaac ttgccagtga
     6481 cgattccgcc gagttgagtc tgctcggcaa tcgttatgtg ttaggcgaag gtttttcaat
     6541 cgccaccgaa gtcaatgcgc gcgacctgca agataactta cgctggatcc gcgaagcgcg
     6601 ccgccaagcc gactgggttg tcgtcagcgc ccattgtcat gaatttggcg gcagcagtct
     6661 gctgaccgcg agcacccggg cggagttgga agagaccgcc gacttcgtca ccaagttcgc
     6721 ccatcaagcc atcgacgaag gcgccgacat tttcgtcggc catggctcgc acttccccat
     6781 gggtgtggaa atttataaag gcaaaccgat cttttacagc gtcggcaatt tcgtctttca
     6841 aaacgaaacc gtcggcttct tccccgccga cgcctacgag cgtttcgatc tcgacctcaa
     6901 ggcgacgccg tccgacttcc tcgacgcccg caccaacggc ggcaaaaaag gccaccccag
     6961 cgatcccgcc tattgggaaa atatgtttcc ggtctgcgag ttcaacaagc agaaattgac
     7021 cagcatcaaa atctacccca tcgatcaagg tttcggccgg ccaagaccgc aacgcggccg
     7081 ccccatgctc gccgaagggg aagtggccga gcgggtgatc gagcgagtag tgcgcctctc
     7141 gcaacgttat gggacgaagg tggtaaatcg agcaggcctc ggcttgatcg aactatagac
     7201 gatgactctg gaagtgtccg gattcaaaat ctgacagcgc tagcggaacg cgaccctgcg
     7261 tcgtgaagac agctaggaat gcgattacaa tgtcgcattc gcctcgccag tcaatgggtt
     7321 aatgtcgcgg gcgatttcgt gcggcgtcaa tcgtcgccag ccgctcaggg acaatcgcta
     7381 tcgacttcac gatggaaggt ttagttctcg ctggcacaat caattgccac tgcgaatctt
     7441 cggttcctat tccgccatga tgtccttcac tcgtttgacg acatcggcgg gttgactgac
     7501 gacggc
//
LOCUS       DASQKS010000236         5995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4741451, whole genome shotgun sequence.
ACCESSION   DASQKS010000236 DASQKS010000000
VERSION     DASQKS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4741451"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VE170_06155"
     CDS             <1..599
                     /locus_tag="VE170_06155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HYJ15054.1"
                     /translation="RFIRPSRVGKQFWVTPPWRTPPRFRSRQVITIEPGMAFGTGTHA
                     TTRGCLEFIEEVAASCASVKLTALDVGTGSGILAIALAKLGAKRVWALDNDPVALGVA
                     RENLRINDVADKVELSGTALRRLKRKFPIVVANLTAETIVDLAGAIAPRVAPAGYLIL
                     SGILRHKAASVLRCFAPRFDVVKRKPAREWVTMLLRRH"
     gene            606..1346
                     /locus_tag="VE170_06160"
     CDS             606..1346
                     /locus_tag="VE170_06160"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HYJ15055.1"
                     /translation="MARFFIRKENIHDADATIAGQEFEHLRRVLRLTVGDSVTLFDDT
                     GWEYEAVIRSLHADRGELAISRAYQAGRDSPLALTLALGLTKGEKMDFVVEKATELGV
                     ASILPFTSSFTVPKLDDRKIANRTERWQKIALSAAKQCGRNRVPEILPLCEFRHMVDV
                     VGASQLKLLFWEKETAQSLRQVHEVNASAAAILVAVGPEGGFSADEAHLAAARDFRLV
                     HLGRRILRAETAAVTALSLAQFLWGDLT"
     gene            1441..2094
                     /locus_tag="VE170_06165"
     CDS             1441..2094
                     /locus_tag="VE170_06165"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYJ15056.1"
                     /translation="MAADELCGECGAKLLHRRVFLGVPKPDSFILTAEENKPDVEDDV
                     ERDDWQFVNRAELKRSGMVIRPTAEPVAAVRWGGFFRRAIALIIDLFVISLLSSIMGA
                     MAYVGYKVGLGAHGQVISSDNVAALFAILTLGALGLTAAYFIVMHGLGGQTIGKRLLG
                     LRVVGVERQPPSFRQAALRWLAAVGFAPIGLGFLWVLWQSEKRAWHDFVARTWVVRE"
     gene            2127..3080
                     /gene="gshB"
                     /locus_tag="VE170_06170"
     CDS             2127..3080
                     /gene="gshB"
                     /locus_tag="VE170_06170"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HYJ15057.1"
                     /translation="MRIGVVMDPVEHIDIDKDTTFVLMLEAQRRGHELYFMEVDDLFV
                     RGGATHGRYRRLELARATPHAHLGEFATGSLADFNSVWMRKDPPFDMKFFFATHLLSL
                     IDEKKCFVMNNPKGLREANEKLYAMRFPEQIPQTLVSSNMERLRSFMDELGGELIVKP
                     LDGCGGTGVFYLNGQDRNTNSILEAATDHGRRMVMGQRYLPEIRQGDKRIIVLNGEPL
                     GAVLRVPLESETRGNIHVGGQCVKTEVTERDREICAALAPLLRADGLYFVGLDVIGNF
                     LTEVNVTSPTGIQEINCLDGVRLESQVIDFVESQVKKLAKA"
     gene            3134..3206
                     /locus_tag="VE170_06175"
     tRNA            3134..3206
                     /locus_tag="VE170_06175"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3167..3169,aa:Phe,seq:gaa)
     gene            3767..4831
                     /locus_tag="VE170_06180"
     CDS             3767..4831
                     /locus_tag="VE170_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15058.1"
                     /translation="MKFSLLLSWSGWRLLGAVLILQFLLPSWVHAQAEPFYKGKQIRI
                     ITGATAGGFYDRWARLLARAMPKYIPGQPDIIVQNMPGAGSLVALNYIHGVVKPDGLT
                     TVMPNSNVYLEQLSGHKEVRFDLRKFSIIGSQEKNYMLLYMRADAPYKTIGDIIKAKE
                     APKCGSPGVGSAGYILDRVLDFVFGAKINTIMGYPGQSEIDLAVEKGEIQCSGNTVLP
                     HFGREPFYTWHKKGFDRHLIQTARKRDPVVSEAPTIYELMDEYKTSESNRRLAQVLLG
                     GAEFGRFMLVAPGTPPDRIKMLREAYARAMKDPELIAEAKKGRMDMDPSTGEELQALL
                     QEIMEQPPEVVERMKRILSE"
BASE COUNT         1354 a         1564 c         1718 g         1359 t
ORIGIN      
        1 agcgatttat ccggccgagc cgggtcggca aacagttttg ggtgacaccg ccctggcgaa
       61 cgccgccgcg gtttcgctcg cgccaggtga ttaccatcga acccggcatg gctttcggca
      121 ccggcaccca tgcgaccacg cgcggctgtt tggaattcat cgaggaagtc gcggcatcgt
      181 gtgcatcagt gaaactcacc gccctcgacg tcggcaccgg gtcgggaatc ttggcgattg
      241 cattggcaaa gttgggagcc aagcgggttt gggccctgga caacgatccg gtggcgcttg
      301 gcgtggctcg ggaaaattta cgaatcaacg atgtcgcgga caaggtcgag ctgtccggca
      361 cagcgctcag acgtcttaaa agaaaatttc ccatcgtggt cgctaacttg acggcggaga
      421 ccattgttga tctcgccggc gccatcgcgc cgagagttgc gcccgccggt tacttaattc
      481 tctccggcat cctgcgccat aaggcggcgt cggtgttgcg ttgttttgcg ccgcgctttg
      541 acgtcgtcaa acgcaaaccc gcgcgcgagt gggtgactat gctgctgcgg cgacactagc
      601 aaccgatggc tcgtttcttc atccgcaagg agaatatcca tgatgccgac gcgaccatcg
      661 ccggtcagga gttcgagcat ttgcgccgcg tgctgcgcct gaccgtgggc gacagcgtga
      721 cgctgttcga tgataccggc tgggaatatg aagcggtgat tcgcagcctc catgccgatc
      781 gcggcgaatt ggcaatcagc cgcgcctatc aagccggcag ggattcgccg ctggcgctga
      841 ctttggcgct ggggctcacc aagggcgaaa agatggattt tgtcgtcgag aaggcgaccg
      901 agcttggcgt agcttcgatc ctgcctttta cctcgagttt taccgtgccc aagctcgacg
      961 ataggaaaat cgccaatcgc accgagcgct ggcagaagat tgcgctgagc gcggcgaaac
     1021 agtgtggacg gaatcgggtg ccggagattt tgccgttatg cgagtttcgt catatggtcg
     1081 atgtcgtcgg cgcaagccag ctcaagttgt tattttggga aaaggagacg gcgcaatcgc
     1141 tgcgccaggt tcacgaggtg aatgccagcg ctgccgcgat tctcgttgcg gtcggtcccg
     1201 agggcggttt tagcgccgat gaggcgcact tggcggcggc gcgagatttc cgcctggttc
     1261 atctaggccg gcgcattcta cgcgccgaga ctgcggcggt gacggctttg tcgttggcgc
     1321 agttcctttg gggtgatttg acttagtgcg gcttgccttg gtagggcgca actcttagca
     1381 tgtggtaaac aaatgttcgc cgaacaaaac ttgaaatgcc agaactgtga agctcccgtc
     1441 gtggcggctg acgaactctg cggcgagtgc ggtgccaagc tgttgcatcg ccgggtgttc
     1501 ttgggtgtgc cgaagccgga tagttttatt ttaactgccg aggagaacaa gcctgacgtt
     1561 gaagatgatg tcgagcgtga cgactggcag ttcgtaaacc gtgctgagtt gaaacgctcc
     1621 gggatggtga tacggccgac cgcagaaccg gtggctgccg tgcgctgggg cggctttttt
     1681 cgccgcgcca tcgccctgat catcgacctg ttcgtgatat ctttgctatc gtcaatcatg
     1741 ggtgctatgg cgtacgtggg ctacaaagtc ggcctcggcg cccatgggca ggttatctca
     1801 agcgacaatg tcgccgcact attcgctatc ctcacactgg gcgcgctcgg cttgaccgcc
     1861 gcctatttca tcgtcatgca tggactgggt ggtcagacca tcggcaagag gttactcggt
     1921 ctgcgcgtcg tcggtgtcga gcgccagccg ccgagcttcc gccaagcggc cctgcgctgg
     1981 ctcgcggcgg tcggcttcgc acccattggc ctcggctttc tctgggtgct ctggcagtcc
     2041 gagaagcgcg cctggcacga ctttgtcgcg cgcacctggg tcgtgcgcga gtaaacttcg
     2101 taggggatgc attcgtcatg agtcaattgc gtatcggggt ggtgatggac cccgtcgaac
     2161 acatcgacat cgacaaggac accaccttcg tgctgatgct cgaagcccag cggcgcggcc
     2221 atgaacttta tttcatggaa gtcgacgacc tgttcgtgcg cggcggcgcc acccatggcc
     2281 gctatcgccg ccttgaactg gcgcgggcca cgccgcacgc gcacctgggt gagtttgcca
     2341 ccgggtcgct ggccgacttt aactcggtgt ggatgcgcaa ggatccgccc ttcgacatga
     2401 agtttttctt tgccacccat ctgcttagcc tgattgacga aaaaaaatgt ttcgtcatga
     2461 acaatcccaa agggctgcgc gaggccaacg aaaaattata cgccatgcgt ttccctgaac
     2521 agatcccgca gacgctggtc agcagcaata tggagcggct gcgctcgttc atggatgaac
     2581 tgggcggcga gctgatcgtc aaaccgctcg acggctgcgg cggcacaggc gtgttctatc
     2641 tcaacggcca ggaccgcaat accaattcga tcctcgaagc ggccaccgac cacggccggc
     2701 ggatggtgat ggggcaacgt tatctgccgg agatccgtca gggcgataaa cgcatcatcg
     2761 ttctcaacgg cgagccgcta ggcgcggtct tgcgtgtgcc gctggagtcc gagacccgtg
     2821 gcaacattca tgtcggcggc cagtgcgtca agactgaagt gacggagcgc gaccgggaaa
     2881 tttgcgcggc gttggcgccg ctcctgcgcg cggatggact ttacttcgtc ggtctggacg
     2941 tgatcggcaa ctttctcacc gaggtcaatg tgactagccc caccggcatc caagagatta
     3001 attgtttaga cggcgtgcga ctcgaaagcc aagttatcga tttcgtcgaa agccaggtca
     3061 aaaagctcgc caaagcctaa gccaaacttg actcgccgaa acttttaagc aatattccag
     3121 attcacttct tttggccagg tagctcagtc ggtagagcag gagactgaaa atctccgtgt
     3181 cggcggttcg attccgtccc tggccacatt taacccctta acatctaggg atctgaaagc
     3241 ttgctgccat ggtgcgattc ttagaccgat taaaatgtca tacaactaag aacaattgca
     3301 ctctgcgaat ctataaagtc attaattaaa tcctacccag ctgtttatcg ccgtcttcta
     3361 taagatgctg ccgacatgtc cgtagcgtcg atatccggtg acctttacca tcaaccattt
     3421 cgccgatcat tcagtgatcc atttgccggc gacttcgcca aaggtcagca cagtcttcaa
     3481 tgtaatatag gcattattgc cgacggcggc gagatgacct acgacaatga tgaagaattt
     3541 gtaatgaatt gctggggaat tcagttcaat ccgagagtta acatttaagg ctgaacgagc
     3601 gagcccagca agcgaagcgc gcgggatcga agattagttt caagtgttgt aagacggcat
     3661 gcccttcgcc tgggacatat aatatgggta acgagtattc aggaaaagct caactggcgg
     3721 tgcaaggggg cagggcggaa acctggtaat ctatgcagga gcaaagatga aattttcatt
     3781 gttgttgtca tggtcgggtt ggcgattgct tggggccgtt ctaatattac aattcttact
     3841 tccaagctgg gttcacgctc aagccgaacc tttctacaag ggaaagcaga ttcgtatcat
     3901 cacaggagcc acagcggggg gtttttacga tcgctgggcg cggctgttag cccgagcaat
     3961 gcccaagtat atcccgggac agccagacat catcgtgcag aatatgccgg gcgcgggctc
     4021 gctggtggcg ctcaactata tccatggcgt ggttaaaccc gatgggctga caaccgttat
     4081 gcccaacagc aacgtctatt tagagcagct ctcgggtcat aaagaagtgc gcttcgactt
     4141 gcgaaagttc tcgatcatcg gctcgcaaga gaagaactat atgttgctct atatgcgcgc
     4201 ggacgcgccg tacaagacca tcggcgacat catcaaggcc aaagaggcgc cgaaatgtgg
     4261 cagcccgggc gtgggcagcg ccggctatat tctcgaccgg gttttggatt tcgtttttgg
     4321 cgcgaagatc aacaccatca tgggttatcc aggccagagc gagatcgact tggcagtgga
     4381 gaagggcgag atccaatgca gcggcaatac ggttttgcca catttcggac gcgagccctt
     4441 ttacacttgg cacaagaaag gattcgaccg ccatttaatt caaaccgcgc gcaaacgaga
     4501 cccggttgtg agtgaggcgc caacgatcta cgagttgatg gacgaatata aaaccagcga
     4561 atcaaatcgc cggcttgccc aggttttgct cggcggcgcc gagtttgggc gctttatgct
     4621 ggtcgctccg ggcacacccc cggaccgaat aaagatgctc cgggaagctt acgcccgggc
     4681 gatgaaggac cctgaactga tcgccgaagc caagaaggga cggatggaca tggatccttc
     4741 aacgggcgag gaactgcaag cgctgctgca agagattatg gaacagccgc ccgaggtcgt
     4801 cgagcgaatg aaaagaatcc tatctgagta gtcgaaccgc gaagccgatt aacgcttact
     4861 gtgcggtctg cggctatcag ctaaaaggat ggcggtggat cgtcgggcgc aagcgaccag
     4921 ttaatatcta gtatggcagg atggcgaagg tgttcagcta atggctcgct atctaattca
     4981 caactgttct cgttgtcgtg ggttaatgag tgtggtggtt cagtgcccta aaagtatgtc
     5041 cttgcaagcc gtcaacggta agtgcgttaa atgtcggcac cggctggcgt ggatcgtaat
     5101 acatcagtga gtatacgatc cgctaagacg atagtcctca ccgattttgc cacagtccct
     5161 attatcactt tcccatgaaa gggacacctg ttttggcgtc tgtttctaat tgctcaaaaa
     5221 tattggggtt agtgttaaca aagctaaggc attggttttg cacaccacat tccggtcaaa
     5281 ggaatataga gtcgatagtc taatacgttc ttggattttc tttagcgccc cgttgacaat
     5341 tctcttaagc aaggcaaagt aatccgcgag cattaaattc ggtacaacaa tgggatgaga
     5401 atagtcgatg ggtctttcac tgggaaagac ggcatccacc gttgtgagca cgccgccaag
     5461 atcagaaccc tgtggcaatt gatctaatag gcactgatgt cagaccgtga ccattcaaga
     5521 agaacactca taaccggcct tcaaggaatc taaatacttt tattgaatcg tcaaggattg
     5581 taagggaaca acgaacgaac tttaaaatgg gcggcatgag acttaatcat ttacttgctg
     5641 cgcttccaaa gtcaaaacta tcagacaccg tgcacagcgg gcatacaacc ggctttttca
     5701 acgaagatac aaggaactaa aagctgactt ttgcgacgag tgcagtaagt cttaatcgaa
     5761 ttcatacaca agcatttttc agcacgttcc gtaatcaaca catccaccta gtggagagat
     5821 tggtcaggct agttaacact gcgcgagcgg aaggagcgag cgaggtcatt gaacggcaaa
     5881 gccagaaatc gccttatcga actaatgaac ttatctagac ctacgtctac gactttttgg
     5941 gttgtatggc attcttccgc cttgattctt gtcacgctta tctggggcgg cctgc
//
LOCUS       DASQKS010000237         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3902120, whole genome shotgun sequence.
ACCESSION   DASQKS010000237 DASQKS010000000
VERSION     DASQKS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902120"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..726)
                     /locus_tag="VE170_06185"
     CDS             complement(4..726)
                     /locus_tag="VE170_06185"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15059.1"
                     /translation="MNRPIKPDLLHIGFVSPGPATWPHYDGFMKLVPDFVEFDFQGLE
                     LFGQSLYEITGKKLAVVRHVTELAAEKKWHAVILIGAPTEVMNPGLFADLKASLQIPV
                     TTALTASVHALQTYGAKRLLLLTPFETRLNDMIEEYLTEAGFSVRAPKSFAHIGEAGR
                     LGPAAVYDLTVQALAEVGSVDAIYFQGAVLDPLPVLQQIENDLEITAVASNTAMLWEI
                     LSQLGRRAPIQGYGKLLAEWPS"
     gene            834..1346
                     /locus_tag="VE170_06190"
     CDS             834..1346
                     /locus_tag="VE170_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15060.1"
                     /translation="MTNPSPILLEDRLSELMRHLGVERAHFAACMPRDWAGLLTRHKH
                     RVASLTLMCPMGIELAPLIDSPPPILIVTGNQGRAGADAIRDGKKITGAVSLVLENYF
                     SPPWADPIRDRRDAIGSAMTHFIESHADGDLQSVATLSSAAGESAGITYSCHGSGAPL
                     LLMPLALSPS"
     gene            1368..2162
                     /locus_tag="VE170_06195"
     CDS             1368..2162
                     /locus_tag="VE170_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ15061.1"
                     /translation="MSEDFRVIILGGAHLGMVAHLETRAQSSYRRVIDQLFDELDLKP
                     GQSLLEVGCGSGAIVRRLAGRSRGSNPIFAADVNSYLIREAAALAELEGLDGYIEFRP
                     GDATHLPFENDRFDCTISCTVMEEGDADRMLGEFVRVTKPGGKMGSVTRSTDLPRWVN
                     LPLTAQLKAQIESPKFFAGNVTDAGCADASLYRRMHEAGLKDVAMLPQWASHRDGERL
                     DYLHERIVALLKPAELIEWRAALDQATVEGTFFIAEPFHCAVGRKQ"
     gene            complement(2230..3219)
                     /locus_tag="VE170_06200"
     CDS             complement(2230..3219)
                     /locus_tag="VE170_06200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYJ15062.1"
                     /translation="MASHLKFGLLLPHFGAHMSVADCLDGARRAEAYGFDSVWVRDHL
                     VFKPHAGEGGDPSHLECLMLLAALAPVTTRVQLGTAMAICHRHPIHLAQSCAALSAIS
                     NGRMILGLGLGGFPHEFAAAGRPSGLAERAELARINIEVCRALWRGETLSHRSAYFDF
                     NDVALKPPPVKSVPIWIGGSTPAACRRAVALGDGWMPARINFPTFRKRIAYLRSLCAE
                     TGRTMVDTAVMPFTTIGKNAEDALRGIDINGLLDEAHNASTWIKPPSGKFATLEDLGG
                     VLIAGAPADIVRESATYEVAGADHIVYDLRLRFNDWYAQIDLLGQEVLPALRS"
     gene            complement(3285..3809)
                     /locus_tag="VE170_06205"
     CDS             complement(3285..3809)
                     /locus_tag="VE170_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15063.1"
                     /translation="MKSVGNFALVLAFLLLGACSYPMTMKSSDGVTYGGRYRFAREDS
                     GLIQIHGPEDEVLLGSFNRVGRTAFVENYEKAFGRGTIELDGPDLSRHGGTIGGILGS
                     ISGFHETAYADPAAAAEGRPSKSITGPLFYWVASLQGDRRTVIGCFLIGSSYTGSGFG
                     RCKSQSGQEYNIDF"
BASE COUNT          890 a         1182 c         1188 g          826 t
ORIGIN      
        1 tgtttaactt ggccactcgg ccagcagttt gccgtaacct tggatcggcg cgcgccggcc
       61 cagttgtgaa agtatttccc agagcattgc cgtattgctg gcgacggcag tgatctctag
      121 atcgttttcg atctgttgca aaaccggcaa tggatcgagc acggcgccct gaaagtatat
      181 ggcgtcgacc gagccgactt cggccaacgc ttgtacggtg agatcgtaga ccgctgccgg
      241 acctaaacgg ccggcctcgc cgatgtgggc gaacgactta ggcgcgcgca ccgagaatcc
      301 cgcctcggtc aaatattctt cgatcatgtc attcaaacgg gtctcgaacg gtgtcagcag
      361 cagtaaacgc ttggcgccgt aagtttgcaa cgcatgcacc gacgcggtca aagccgtggt
      421 cacagggatt tgcagggatg cttttagatc ggcgaacagg ccgggattca tcacctcggt
      481 gggcgcgccg atgaggatga ccgcatgcca tttcttttca gcggcaagtt cagtgacgtg
      541 gcgcacgacc gcgagtttct tgccggtgat ttcgtaaagc gattggccga aaagttccaa
      601 cccctggaaa tcgaattcaa caaagtccgg caccagcttc ataaagccat cgtaatgcgg
      661 ccaggtcgcc ggtccggggc tgacgaaacc gatatggagc aagtcaggtt taatcggccg
      721 attcatcgct tctgtagatc aatagcatag agggtcaaga aaaacaggtt cggcggtgag
      781 ttgtattagc gctgaaggcc gagctaagtg taaacgagat ggagacgact cgaatgacga
      841 acccatcccc tattctcctg gaagatcggc taagcgagct catgcgtcat ctcggcgtcg
      901 agcgcgctca tttcgccgcc tgcatgccgc gcgactgggc aggactgctt acccgccaca
      961 aacacagggt cgcgtcactc acgttgatgt gcccgatggg cattgaactg gcgccattga
     1021 tcgacagccc accgccgatc ttgatcgtta ccggcaatca agggcgcgcc ggcgccgacg
     1081 ctatcagaga cgggaagaag atcaccgggg cggtttccct ggtattggag aactacttta
     1141 gcccgccgtg ggccgatccg attcgcgatc ggcgcgacgc catcggtagt gcgatgacac
     1201 atttcatcga aagccatgct gacggcgatt tgcagtccgt ggcaacccta tcatcagccg
     1261 ccggcgaatc tgccggcatc acttattcct gtcatggcag cggcgcgccg ttattgctca
     1321 tgcccctggc cttgtcccct tcctaatggc aaccgcttat cgagcaattg agcgaagact
     1381 ttcgcgtcat tattttgggc ggcgcacacc tcggcatggt cgcccatctg gaaactcggg
     1441 cgcaatcgag ttacaggcgc gtcatcgatc aattgttcga cgaattggat cttaagccgg
     1501 gacaatcgtt gctggaagtc ggctgcggct ccggcgccat cgtgcgtcgt ctggccgggc
     1561 gcagccgtgg cagcaatccg attttcgcgg ccgacgtgaa tagttatctc atacgcgagg
     1621 cggcggcgtt ggccgagctg gaaggtttgg atggatatat cgaattccgt cctggcgacg
     1681 ccacccacct gccgttcgaa aacgatcgat tcgactgcac gatttcatgc accgtgatgg
     1741 aggaaggcga cgccgaccgc atgctcggtg aattcgtccg ggtcaccaaa cccggcggca
     1801 aaatgggcag cgttacccgc tcaaccgacc tgccgcgctg ggtgaatttg ccgttaaccg
     1861 cgcagctaaa agcccagatc gagtcgccaa agttttttgc cggcaacgtc accgacgcag
     1921 gctgcgccga cgccagcctc taccggcgca tgcatgaagc gggcttaaaa gatgtcgcga
     1981 tgctgccgca atgggcgagt cacagggacg gcgaaaggct ggactatctg cacgagcgta
     2041 tcgtcgcgct cctcaaaccg gccgaactca tcgaatggcg cgcagctcta gatcaggcca
     2101 cggttgaagg gacgtttttc atcgccgaac cgtttcattg tgcggtggga agaaaacagt
     2161 aaatatacag gagtagccgt cagcaatccc ccccacctcc ttgctcctaa ctactcactc
     2221 cttactgttt tatgagcgca gcgccggcag cacttcttgc cccaacaaat cgatctgcgc
     2281 gtaccagtcg ttaaagcgca gccgcaagtc gtagacaatg tgatcggcgc cggcaacttc
     2341 gtaggttgcg ctttcgcgca cgatgtcggc gggagcgccg gcgatcagca cgccgcccag
     2401 gtcttcgaga gtggcaaatt ttcccgacgg cggtttgatc caggtggacg cattgtgggc
     2461 ttcatccaac aagccattga tgtcgatgcc gcgcagcgca tcctcggcgt tcttgccgat
     2521 ggtggtaaac ggcatcaccg ccgtgtcgac catcgtccga cccgtctcgg cacagagcga
     2581 acgcagatat gctatccgtt tgcggaacgt cggaaaattg atccgcgccg gcatccagcc
     2641 atcgccgagc gcgaccgcgc ggcggcaagc cgcgggcgtg ctaccgccga tccagatagg
     2701 aacggatttg acaggaggcg gtttgagggc gacatcgtta aaatcaaaat aggcgctgcg
     2761 gtgggataga gtttcgcctc gccacagagc gcggcagact tcgatattga tccgcgctag
     2821 ttcggcgcgc tcggcaagcc cactcggccg gcctgccgcg gcaaattcat ggggaaagcc
     2881 gcccaaacca agacctaaaa tcattcgccc gttagagatg gcgcttaacg cggcgcagga
     2941 ttgcgccagg tgaatcggat ggcgatggca aatcgccatg gcggtgccga gttgtactct
     3001 ggtcgtcacc ggcgccaggg ctgcgagcaa catcaggcat tccagatggc tcggatcgcc
     3061 gccttcgccg gcgtgcggtt tgaaaaccaa atgatcgcgc acccacaccg aatcgaaacc
     3121 gtacgcctcg gcgcgccgcg caccatcgag gcagtcggca accgacatat gggcgccaaa
     3181 atggggcagc agcagaccga atttgaggtg tgacgccatg agattattta ttgcaagtat
     3241 atcgatgatg acgtcgtgtc agggtctgca gcgaaaacca ttactcaaaa atcgatgttg
     3301 tactcttggc cgctctggct tttacagcgg ccgaagccgc tgccggtata tgacgagccg
     3361 atgagaaagc agccaatcac ggtgcgtcga tcgccttgca gcgaagcaac ccaataaaac
     3421 agcggcccgg tgatcgactt actcggccgt ccctcggcgg cggcggcggg atcggcatag
     3481 gccgtttcat gaaagccgga tatcgatccc agtatcccgc ctatagttcc gccatggcgc
     3541 gacaggtcgg ggccgtcaag ttcgatggtg ccgcggccga aggctttctc gtagttctca
     3601 acaaatgccg tgcggccgac ccggttgaag ctgcccagca gcacttcatc ttccggccca
     3661 tgaatctgta tcagaccgct gtcctcgcgg gcgaaacggt aacgcccgcc gtaggtcacg
     3721 ccgtccgatg atttcatagt catcggatag gaacaggctc cgagcagcaa gaatgctaaa
     3781 accagagcga aatttcccac cgacttcatt gggcaaatat taacacgctt tgcgtcggac
     3841 aagaaatcac cggcgaaatc acggctaaag acttcctgcg ctttgccaaa tcgtgctaaa
     3901 ggtcgagttc gacggagact ctccaagcag gagtgatttt ttcacgtggc acaaaatcca
     3961 cgtatcgaag ctttgatggc ggcgtttcgt aagaccactt attttcgctg gatggccaag
     4021 gaagccattc cggtggtcga cggttatggc gtcgaagatg tgcgcgacgt cgcgatggcc
     4081 ccttgg
//
LOCUS       DASQKS010000238         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6013798, whole genome shotgun sequence.
ACCESSION   DASQKS010000238 DASQKS010000000
VERSION     DASQKS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6013798"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VE170_06210"
     CDS             <1..312
                     /locus_tag="VE170_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15064.1"
                     /translation="VRRYMMAQTEAIARAKRDKNMALKVMGKYLRTTNPNALAESYDI
                     DVQKYLLRVPLTTAEAMRSVLDDLAGRIPKAKDADPKRFFDDSFVRQMQSSGFIDALY
                     K"
     gene            413..1549
                     /locus_tag="VE170_06215"
     CDS             413..1549
                     /locus_tag="VE170_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15065.1"
                     /translation="MRVIDADGHVEENPLTFSDKYFDPAFRAHRPVVVPGTEEGLAYW
                     MIDEQLFPRRVGRGCNNLGTPASYNGKPVRHAQRKPDTLGSMELTHLSERLQIMDEEN
                     IDLQVLYPTLFLAYPLSSNPAYTSAMCHAYNRWLGDVLSGQKRLKWVGVANLDDIPGA
                     VKQVKEAKQLGAVGMMILGTAGDRLLDDDNLMPFYEAVASEALPLAVHVGWACPALNN
                     LYTHIYPSGVIPFLMPVLMGMVSMMSGGIFDRFPSMRVVYLEAGCLWVHFILERLHHR
                     FEHSGKNLSNVVSRTAPIQKLAPMEYIKRGNIYFSSEVEDILLPHVLELVGDGQMMFA
                     SDMPHGDRERFAAKTLQDRNDITEAAKTKILESNPVTFYRLPVF"
     gene            1660..2520
                     /locus_tag="VE170_06220"
     CDS             1660..2520
                     /locus_tag="VE170_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ15066.1"
                     /translation="MAEYYAFQAALCFSIAHIFVRRGLTGSNALTGSVISLGTSAVIF
                     WTLASLLAPLSFLATPAIGYFMAAGFFAPAIGQTLGYIGMERIGVARSSPIVNTSPMF
                     SSILAVLFLGEIWLGQNILGTSLVILGVVILSSSRPPTGDWNKKDVIYPLLAAVAFGI
                     STTLRKSGLMMVPVPLLAAAVTVGTALIVLLAIVWLRGGGKILRFNRQSSAWLFGGAL
                     INSAAILSFFAALNVGAIVRVEPLVACNPLLTLVWASIFLRQFETLSLRVVTGALVTV
                     TGTVLVVTVN"
     gene            2520..3386
                     /locus_tag="VE170_06225"
     CDS             2520..3386
                     /locus_tag="VE170_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ15067.1"
                     /translation="MSMTVYFSFQAALCFSIAHILIRRGLVGSNAMTGSLISLTMSAV
                     VLWCALPFFVPLSALANPALWIFVAAGVFAPGIGRTLSYVGIEKIGVARSVPIANSSP
                     IFASIFAVIFLAESWVLQNIAGTLLVIGGVIVLSMAKPAQGKWRKIDVIYPLVGAVAF
                     GASAILRKAGLGLVNIPLLAAAVTAGTAALFSFALLQIRGGRQAFKLNPRSASWLFSA
                     GVFNTAAMLSVFFALSHGKVVIVEPLVSSNPVTTLLLTSIFLRDVESLTLRVILGAAL
                     TVIGTILVVLVK"
     gene            3454..>4291
                     /locus_tag="VE170_06230"
     CDS             3454..>4291
                     /locus_tag="VE170_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15068.1"
                     /translation="MSKAMQAERRMWSKFYSYDEWMEAQGIPIYRGYYIEDLRTLELK
                     WWDARECNSAFIQLVGQEGVTSARVTEIPPGKSLPPLKFALDEIVYVVEGRGLSTIWS
                     GEKRPKKTFEWQKHSMFLLPHNYTHQFTNMQGDKPVRLLHYNYMPLTLSGVRDVNFIF
                     ANGYEGADIHNEMDFYSEAKMVSQNLLDDSWGRRAYWYGNFFPDMKAWDKLDSNARRG
                     AGGRSVYIQFPDSEMSCHMSVFDARLYKKAHRHGPGRVIVIPVGEGYSIMWEEGKDKV
                     VVP"
BASE COUNT          851 a         1228 c         1201 g         1011 t
ORIGIN      
        1 gtgcgccgtt acatgatggc gcagaccgaa gcgatcgccc gcgccaagcg cgataagaat
       61 atggcgctca aagtcatggg caaatatcta cgcaccacca accccaacgc gctcgccgag
      121 tcctatgaca tcgacgtgca aaaatacctg ctgcgcgtgc cgctgaccac ggcagaagcg
      181 atgagatcgg tgctcgacga tctcgccggg agaatcccta aagccaagga cgccgacccg
      241 aagagatttt tcgacgacag tttcgttcga caaatgcagt cgagcggttt catcgacgcc
      301 ctctacaaat agtcgcgccc atcgtccaca ccgttagaat cgcatccgta gcggtttgcc
      361 cggcgagctt gcctgagata agaatttacg atagttccgg aggtggtatt tcatgcgtgt
      421 catcgatgcc gacggccatg tcgaagaaaa tccgcttacg ttcagcgata agtatttcga
      481 cccggccttt cgcgcccatc gtccagtggt cgttccagga accgaagaag gtctcgccta
      541 ttggatgatc gacgagcagc tgtttccccg ccgtgtcggc cgcggctgca ataacctggg
      601 cacgccggca agttataacg gcaaaccggt gcgtcatgcc cagcgcaagc ccgataccct
      661 cggcagcatg gagctgaccc atttaagcga acggctacag attatggacg aggagaacat
      721 cgatctccaa gttctctatc cgacgctgtt tctcgcctat ccgctaagca gcaatccggc
      781 atacaccagc gccatgtgcc atgcctataa tcgctggctc ggcgacgtgc tttccggcca
      841 gaagcggttg aagtgggtcg gtgtggccaa cctcgacgac attcccggcg cggtcaaaca
      901 agtgaaagaa gcgaaacagc tcggcgccgt cggcatgatg attctcggca cggctgggga
      961 tcggctgctc gacgacgata acttgatgcc gttctacgaa gcggtcgcca gtgaagctct
     1021 gcccctggcg gttcatgtcg gctgggcctg tccggcgctg aataacctct acacccatat
     1081 ttatccgtca ggggtgatac cgtttttgat gccggtgctc atgggcatgg tgtcgatgat
     1141 gagcggcggc atcttcgacc gatttccgag tatgcgcgtg gtctatctcg aagcgggttg
     1201 cctgtgggtc catttcattc tcgaacgctt gcatcaccgt ttcgagcatt cgggaaaaaa
     1261 cttgtccaac gtcgtgtcgc gcaccgcgcc gatccaaaag cttgcgccga tggaatacat
     1321 caaacgaggc aatatttatt ttagctctga agttgaagac atattgctgc cgcatgtgct
     1381 cgaacttgtt ggcgatgggc aaatgatgtt cgcatccgac atgccgcacg gcgaccgcga
     1441 acgtttcgcc gctaagacgt tgcaggatcg caatgatatc accgaggcgg caaagaccaa
     1501 gattctggaa tcgaatccag tgacatttta tcgcttgccg gttttctagg cggggccgac
     1561 aagcaccgga ttaacctcgg caatcgatgc cgtcggcgtt tacctcactc ccggcaacat
     1621 gcccttaagc gtagtcttcc tgcgaccgag tcaactccaa tggccgaata ctacgccttc
     1681 caagccgcat tatgtttttc gatcgcgcat atatttgtcc gccgcggcct cactggctcc
     1741 aatgcgttga ctgggtcggt gatctccttg ggcaccagtg cggtgatttt ctggaccctc
     1801 gctagtttgt tggcgccgct ttcgttcttg gcgacgccgg ctatcggtta cttcatggcc
     1861 gcgggcttct tcgctccggc catcggccaa actttgggtt acatcggcat ggaacgcatc
     1921 ggtgttgccc gctcctcgcc gatcgtaaat acatcaccga tgttttcctc gatcctggcc
     1981 gtgctatttc tcggcgagat ttggctgggg caaaacatcc tgggcacctc cctggttatc
     2041 ttaggtgtcg tcatactgtc ttccagccgg ccgccgaccg gagactggaa caagaaggat
     2101 gttatctatc cgctccttgc agccgtggcc tttggcatct cgacaacgct gcgcaagagc
     2161 ggattgatga tggttcccgt gccgttgctt gccgctgcgg ttacggtagg cacggcgtta
     2221 atcgttttgc tcgccatcgt ttggttgagg ggcggcggca aaattttgcg ctttaaccgc
     2281 caaagcagcg cctggctctt cggcggtgca ttgattaaca gcgcagcgat cctttcgttc
     2341 ttcgccgcgc tcaacgtcgg cgccatcgtt cgagtcgaac ctctcgtcgc ttgcaatccg
     2401 ctattgaccc tcgtctgggc ctcgattttc ctgcgccaat ttgaaacgct ttcgctgcgc
     2461 gtcgtgaccg gcgcgttggt gacggtcacc ggaacagtgt tggtggtgac ggtgaactaa
     2521 tgagcatgac ggtctatttt tcgtttcaag ccgcgctgtg cttttccatc gcgcatattt
     2581 tgatccgccg cggcctggtt ggatccaacg ccatgaccgg ttctttgata tcgctgacca
     2641 tgagcgcggt ggtcctgtgg tgtgcgctgc cgttcttcgt gccgctgtcg gccctggcaa
     2701 atccggccct gtggatattt gtcgcggcgg gagttttcgc cccaggtatc ggccggacac
     2761 tcagttacgt cggcattgag aaaatcggcg tcgcccgctc cgtgcccatc gctaattcct
     2821 cgccgatctt cgcgtcgatc tttgcggtga tctttctcgc cgaatcctgg gtgctgcaaa
     2881 acatcgccgg caccttgctg gtgatcggcg gtgtcatcgt tctgtcgatg gcgaaaccag
     2941 cccagggcaa atggcgcaaa attgatgtga tatatccgtt ggtcggcgcc gtggcgttcg
     3001 gggcgtcggc aatcttgcgc aaagccggtc tcggactggt taacatcccg ctgctggccg
     3061 ccgctgtcac cgcgggaacg gccgcgttgt tttcgttcgc gctgctgcaa attcgaggcg
     3121 gcagacaggc tttcaaactc aacccgcgca gtgcatcgtg gctattttcc gccggggtct
     3181 tcaataccgc tgcaatgctg tcggtattct tcgctctcag ccacggcaaa gtggtcatcg
     3241 tcgaaccgtt ggtcagctcc aatccggtga ccacgctttt gctcacttcg atctttctcc
     3301 gcgatgtcga gtcattgacc ctgcgcgtca ttctcggcgc cgccctgacg gtcatcggga
     3361 cgatcctcgt cgtgctcgtc aagtgagcgg ttttaattcg attttccaag gtgatatgac
     3421 tgtcgcaaag aaatctccgg aggaatcacg gtaatgtcca aagcgatgca ggccgaacgc
     3481 agaatgtgga gcaagtttta ctcctacgac gagtggatgg aggcccaagg catcccaatc
     3541 taccgcggct attacatcga agacctgcgg acattggaat tgaaatggtg ggacgcccgg
     3601 gagtgcaact cggcgtttat tcaattggtc ggtcaagaag gagtgacttc agcgcgggtc
     3661 accgagattc cgccgggcaa aagtttgccg ccgctgaaat tcgccctcga tgaaatcgtt
     3721 tacgttgtcg aagggcgcgg cctcagcacc atttggtccg gtgagaaacg gccgaagaaa
     3781 actttcgagt ggcaaaagca cagcatgttc ttgctgccgc acaactatac ccatcaattc
     3841 accaacatgc agggcgacaa gccggtgcgc ttgctgcatt acaattacat gccgctgaca
     3901 ttgtccggcg tgcgcgatgt caattttatt ttcgccaatg gctacgaggg cgccgacatc
     3961 cacaacgaga tggattttta ttctgaagcg aaaatggtct cgcaaaattt gctcgacgac
     4021 agctggggcc ggcgcgctta ctggtacggc aattttttcc cggacatgaa agcctgggat
     4081 aagctcgata gcaacgcccg gcgcggcgcc ggcgggcgca gcgtctacat tcaattcccc
     4141 gactcggaga tgtcttgcca catgtcggtg ttcgacgcgc gcttatacaa gaaggcgcac
     4201 cgccatggtc ccgggcgggt gatcgtcatt ccggtcggcg aagggtactc gatcatgtgg
     4261 gaagagggca aagacaaagt cgtcgttccc t
//
LOCUS       DASQKS010000239         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4742396, whole genome shotgun sequence.
ACCESSION   DASQKS010000239 DASQKS010000000
VERSION     DASQKS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4742396"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VE170_06235"
     CDS             <1..477
                     /locus_tag="VE170_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HYJ15069.1"
                     /translation="AIIVREFDGVAVHTLLNLQRLFPNHYKNVVFVSVGVIDAGQFKG
                     HAEIESLRRKKEEDLKSFVDLAICLGWYAEYRYELGVDLIDELEQICNSVAKEFPRTI
                     FFSGKLVFEEENIFTRLLHNHTPYTLQQKLQFAGREMMILPIRVSLQQNAPARLAA"
     gene            693..1178
                     /locus_tag="VE170_06240"
     CDS             693..1178
                     /locus_tag="VE170_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ15070.1"
                     /translation="MKTTKADLYRGDGAVVALPVVERILHPTDLADTSVRALTLAIKL
                     ARQNHAELLLAHALPPPTPIFEIESFERSSSELDLELLVERLSREGIRARKILIKGTR
                     SVPGNIAECAKFFGADLIVMGTRSRRGLARFFRGSIAACVVKTAPCPVLVVPNRSSAT
                     L"
     gene            1202..2893
                     /gene="kdpA"
                     /locus_tag="VE170_06245"
     CDS             1202..2893
                     /gene="kdpA"
                     /locus_tag="VE170_06245"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HYJ15071.1"
                     /translation="MNGNDWYFLMSFFAVIMIATPLLGGWMARVFSGERHWLSPLAGP
                     VERSIYRLAGVDWQCQMDWKEYLSALAVFNILGFFAVFGLEITQSVLPFNPSALPDVA
                     WPLAFNTAVSFMTNTNWQAYAGEATLSYLVQLLGLTVQNFLSAATGIAGFLAFARGLT
                     QKSSEKIGNFWADVTRAIVHILLPLSMLLAIALVAQGVVQSFSAYVTATTLEGAKQVI
                     PLGPAASQIAIKQLGTNGGGFFGVNSAHPFENPTALTSFLELVAILLIPAALTYTYGK
                     ITGSLQHGWCLFTAMGVLFLSGLAIALWAEQQPNNTLALVSNLEGKELRFGVTGSVLW
                     SVATTAASNGSVNAMHASMAPLAGLAQMFNIMLGEVVFGGVGAGLYGMLIFVIVTVFI
                     AGLMVGRTPEYLGKKIEQREVIWAVIAVILPSATILCGTALSCLLPDALASRANTGPH
                     GFSELLYAWTSASGNNGSAFAGLNANTNFYNIGLALAMLIGRFAVIIPALAIAGSMAV
                     KKTAPPSPGTFPTDGVTFVALLIGVLVIVGGLTFLPALTLGPVLEHFLMLQGRLS"
     gene            2914..>4483
                     /gene="kdpB"
                     /locus_tag="VE170_06250"
     CDS             2914..>4483
                     /gene="kdpB"
                     /locus_tag="VE170_06250"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HYJ15072.1"
                     /translation="MAPKTKAFRVSRELVGHAVLDSVRKLDPREQWNNLVIFVVYFGA
                     ILTTINLLWTFTAFSLQVVLWLWFTVLFANFAESIAEGRGKAQAEALRKMRVATQARL
                     WRDGKESKVTAAELRVGDQVICETGDVIPGDGEVVQGIASVDESAITGESAPVIREAG
                     GDRSAVTGGTRVLSDRIVVRISSEPGKTFLDRMIALVEGAARQKTPNELALEVLLVSL
                     TVLFLLVVITLPAMAWHIENEANQPGAAGMNLFVLTALLVCLIPTTIGGLLSAIGISG
                     MDRLVQRNVLATSGRAVEAAGDIDVLLLDKTGTVTLGNRMATEFISAPGIAPARLADV
                     AQLASLADETPEGRSIVVLAKEQFALRGRELGAAHANFVPFSAKTQMSGVDFPENGKM
                     RQIRKGSAQAIRLHVEGLGASLPAAVRSSVEAIARQGGTPLVVAEGAQVLGVVHLKDV
                     VKGGIKERFAELRGMGIKTVMITGDNPLTAAAIAAEAGVDDFMAQAKPEDKLARIRQE
                     QELGHLVAMTGDGTN"
BASE COUNT          896 a         1202 c         1335 g         1050 t
ORIGIN      
        1 gcgattatcg tgcgcgagtt cgatggcgtt gcggtgcaca ctctgttgaa cctccaacgc
       61 ttgtttccca atcactataa gaatgtcgtc tttgtttcgg tcggcgtgat cgacgccggc
      121 cagttcaaag gccatgcgga aatcgagagt ctgcggcgaa aaaaagaaga ggacttaaaa
      181 agtttcgtcg acttggccat ctgtctgggt tggtacgccg agtatcgcta cgagctcggc
      241 gtcgatttga tcgatgaatt ggagcagatt tgcaactcgg tggccaagga gtttccgcgc
      301 acgattttct tcagcggcaa gctggtattc gaggaagaga acatatttac ccggctgctg
      361 cataaccaca cgccttatac cttgcagcag aaactccagt tcgccggccg ggaaatgatg
      421 atcttgccga tccgggtctc tcttcagcag aatgctcctg cgcggctcgc agcctaagcc
      481 tagccaaaat gatttggcgg cgccgtcgcc tacgaatctt accggcgaaa cattcggctg
      541 gcatccaagt tacgcaccag caagttacct acattggatg agtatctcta tcgccgacct
      601 gttcctgcca gcggattccg ttaacgtaaa ttttatattt tagcccgagt ttttaattag
      661 ccttggtgcg gatcagggat taattttaac gcatgaagac taccaaagct gatctctacc
      721 gaggcgacgg cgcggtggta gctttgcctg tcgtcgaacg gattctacac ccgacggact
      781 tggccgatac atccgtccgg gcgctaactc tggcgattaa gttggcccgg caaaatcatg
      841 ccgagttgtt acttgcccat gcattgccgc cacctacacc cattttcgag atcgaatctt
      901 tcgagcgatc gtcctcagag ctcgatctag aactgctcgt cgagaggctc tcgcgcgagg
      961 gaatacgcgc gcgaaagatc ttgatcaaag gaacccgatc ggtccctggc aatatcgccg
     1021 agtgcgccaa gtttttcggc gcggatttga ttgtcatggg aacacgttct cggcgcggct
     1081 tggcgcgctt cttcagaggg agcatcgccg cgtgtgtggt caagaccgcg ccgtgcccgg
     1141 tgctagtggt gccgaacaga tcgtcagcaa cgctatagtt tatcaaacga gatgaaagcc
     1201 catgaacggt aacgattggt atttcctaat gagttttttt gcagtgataa tgattgcaac
     1261 gcccttactc ggcggttgga tggctcgggt attttcgggt gaaaggcatt ggctctcgcc
     1321 gcttgcgggg cccgtcgaga gatcgattta ccgtctcgct ggcgtcgatt ggcaatgcca
     1381 aatggattgg aaagagtatt tgtcggcact agccgttttt aatattttgg gcttcttcgc
     1441 tgtctttggg ttagagatca cgcagtccgt cttgccattc aatccctcgg ctctgccgga
     1501 tgtagcttgg ccgttggctt tcaatacggc tgtaagcttc atgaccaata ccaactggca
     1561 agcctatgcc ggcgaggcca ccctgagcta cctggtgcag ctcctaggct tgacagtgca
     1621 aaattttctc agtgccgcca ccggtatcgc cgggttccta gctttcgcgc gcgggctgac
     1681 acaaaagtcc tccgagaaga tcggaaactt ctgggcggac gtgacccgag ccatcgtcca
     1741 catccttttg ccgttatcga tgctactcgc gatcgcgtta gtcgcccaag gcgtggtgca
     1801 gtccttctca gcctatgtca cggcgacgac tttggaaggg gccaagcaag tgatcccgtt
     1861 ggggcctgcg gcatcccaga ttgccatcaa acagctgggg acaaacggcg gcgggttttt
     1921 cggcgtcaac agtgcccatc cttttgagaa tcccacggcg ctgaccagct ttttggaatt
     1981 agtcgcgatc ttgctgattc ccgctgcgct cacctacacc tacggcaaga tcacagggag
     2041 tttacagcat ggctggtgtt tgttcacggc aatgggtgtc ctgttcctgt ctggattagc
     2101 aatcgcgctc tgggcggaac agcagccgaa taacacgctc gctttggtca gcaacttgga
     2161 aggcaaagaa ctgcgtttcg gagtgaccgg tagcgtactc tggtcggtcg cgactacagc
     2221 cgcgtccaat ggctcggtta atgccatgca cgcgagcatg gcgccgttag cgggcttggc
     2281 gcagatgttc aacatcatgc tcggcgaggt ggtcttcggc ggcgtgggcg ccgggctcta
     2341 cggtatgttg atatttgtga tcgtgacggt ctttatcgcc ggactgatgg tcggacgaac
     2401 cccggaatat ctcggcaaga agatcgagca gcgggaagtg atttgggccg tgattgcggt
     2461 gattttgcct tcggcgacga ttctttgcgg caccgcgcta tcttgtctgt taccggatgc
     2521 gctggcgagc cgagctaaca ccggccccca cggattctcc gagttgcttt atgcctggac
     2581 gtcggcttcc ggcaacaacg gcagtgcgtt tgccgggctc aatgccaata ccaatttcta
     2641 caatatcggt ttggcgctcg ccatgctgat cggaagattc gccgtcatta tcccggcttt
     2701 ggcgattgcc ggcagcatgg ctgttaagaa aaccgcgccc ccgtcgccgg gcacttttcc
     2761 taccgatggt gttaccttcg tggcactttt gatcggcgtg ctagttatcg tcggtggtct
     2821 gaccttccta ccggcgctga cgttgggtcc ggttttagaa cattttctga tgctgcaagg
     2881 ccgtttgtcg tagatcaagg gagagattct ttaatggcac cgaagaccaa agcattcaga
     2941 gtctcgcggg agctggtcgg tcacgccgtg ctcgacagcg tgcgcaaact ggatccgcgc
     3001 gagcaatgga ataatttggt gatcttcgtc gtctatttcg gggcgattct aacgacgatc
     3061 aatttgcttt ggacttttac cgcattcagc ctgcaagtgg tgctgtggct gtggtttacc
     3121 gtgctgtttg ctaattttgc cgaatcgata gcggaggggc gcggcaaagc tcaagcggaa
     3181 gcattacgca agatgagagt agccactcaa gcgcgtctgt ggcgcgatgg gaaggagagc
     3241 aaggtgacgg cagcggagct gcgcgtcggc gatcaggtta tctgcgagac cggtgatgtt
     3301 atccctggcg atggcgaagt ggttcaaggc atcgccagcg tcgatgagtc ggcgatcact
     3361 ggcgaatccg ccccggtgat tcgcgaagcg ggcggcgatc gctcggcggt taccggcggc
     3421 acgcgcgtat tgagcgaccg tattgtcgtg cgcatctctt ccgaaccagg caagactttt
     3481 ctcgatcgca tgatcgccct ggttgagggt gcagcacggc aaaagacgcc caatgaactg
     3541 gccctggagg tcttgctcgt cagtcttaca gtattgtttc ttttggtcgt catcacattg
     3601 ccggcgatgg cgtggcatat tgagaatgaa gccaatcaac ccggtgcggc gggtatgaac
     3661 ctattcgtat taaccgcttt gttggtttgc ttgattccca ccaccatcgg cggcttgctc
     3721 agcgccattg gcatcagcgg catggatcgg ctggtgcagc gcaacgtttt agctaccagc
     3781 ggtcgggcgg tggaggccgc cggcgatatc gacgtgctgt tgctcgataa aacaggaacc
     3841 gtgactttgg gcaaccggat ggcgacggag tttatcagcg cgcccggaat cgcccccgcg
     3901 cgactggctg acgtagcgca gttggcgtcg ctggccgacg aaacgccgga agggcgttcc
     3961 atcgtcgtat tggccaaaga gcagtttgcc ttgcgcgggc gcgaattggg ggcggcccat
     4021 gctaacttcg tcccgttcag cgctaagact cagatgagcg gcgtcgactt cccagaaaac
     4081 ggcaagatga gacagattcg caagggctcg gcacaggcaa ttcgcctgca tgtggaaggc
     4141 ctcggcgcta gccttccggc ggcggtgcgc agcagcgtcg aggccatcgc gcgccaaggc
     4201 ggcacgcccc tggtggttgc cgaaggggcg caagtgctgg gcgtcgtgca cttaaaagac
     4261 gtcgtgaaag gcggcatcaa agagcgcttc gccgagttgc gcgggatggg tatcaagacc
     4321 gtcatgatca ccggcgataa tccgttaacc gctgcggcga tcgccgccga agccggcgtc
     4381 gatgacttca tggcgcaggc gaagcccgaa gataagctgg cacgcattcg ccaggaacag
     4441 gaactcggtc atctcgtggc gatgaccggc gacggcacca acg
//
LOCUS       DASQKS010000240         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3484942, whole genome shotgun sequence.
ACCESSION   DASQKS010000240 DASQKS010000000
VERSION     DASQKS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3484942"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VE170_06255"
     CDS             <1..718
                     /locus_tag="VE170_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ15073.1"
                     /translation="THRLITPAPRPVTQEDINAAVLHTLETNVLPSAAAKAYEIIRPS
                     IVRVRRMEPNKNGEQDEKGVGTGVVIVDKGVILTNLHVVFGAKRVQVVFADGLESEAT
                     VTGIQPEHDLAVLQAHKIPDDLTAATMRSTADLAIGDQVVAVGYPFGIGPSASAGVVS
                     GLRREFYSPNGKRMLTNLIQFDAAANPGSSGGPLVTDRGEVVGIVTAILNPTAQRFFV
                     GIGFAVPIENAATAVGMSPF"
     gene            750..1763
                     /locus_tag="VE170_06260"
     CDS             750..1763
                     /locus_tag="VE170_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYJ15074.1"
                     /translation="MDRETNHKDVASLMERILYEVKKVIVGQDRFLERVLVAVLAQGH
                     LLVEGVPGLAKTLTVKTLARTIRGSFKRIQFTPDLVPADLVGTRIYNQKTGEFTTTLG
                     PVFTNLLLADEINRAPAKVQSALLEVMQERQVTIAGESHRVTEPFLVMATQNPIETEG
                     TYPLPEAQVDRFMMKVLVGYPTEEEEFVIVDRVTGTSNDVVPLATTEQLCALQRECQR
                     VYVDPALKQYAVRLASATRDPDRFGVAEVAKYVSFGVSPRASIHLVEGARALAFLRGR
                     TYVLSEDVTDIVLDVFRHRLVLTYEALAEGMTPDHLIRRIMEHIPAPEKPLESHVKLG
                     ASL"
     gene            1926..2888
                     /locus_tag="VE170_06265"
     CDS             1926..2888
                     /locus_tag="VE170_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYJ15075.1"
                     /translation="MSSVQESIPTAGAENILRRLEWTVLRRLDGLLHGNYQTLFRGFG
                     LDLADLREYQYYDDVRYIDWNVTARLQTPYVRVYNEDREVMAWFLLDLSPSVDFGARV
                     KKRSVSTEFVTVLTRLLTRHGNRVGALFYGDSVDTVIPARSGRRHALHILHRILSRPE
                     TPPSAATNLGDFLQTALRVMKRRSLVFIVSDFISTPGWAKPLANLTQRHEVIAVRLYD
                     PLEMELPDLGLVMMRDAETGEQLFVDTHDRTFRKRFAVLAERREQELRSAFTQAGVDV
                     LELSTSDDLANAILRFADLRKRRSQLAAGGLPQHLRGSHDVSLA"
     gene            2869..3912
                     /locus_tag="VE170_06270"
     CDS             2869..3912
                     /locus_tag="VE170_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ15076.1"
                     /translation="MTFLWPEMFWLLLVVPALVAGYFYLLRRKQQTALKYASLSMVKE
                     AMGAGQRFRRHIPPLLFLLALIAMLVAVARPAAVVTLPSQHQTIILAMDVSGSMRAVD
                     VQPNRITAAQAAAKAFVAEQPSNARIGVVSFAGTAAVVQVPTHNREDIIAAIDRFQLQ
                     RGTAIGSGIIISLATIFPDAGIEVNASNYGRYAPRGMALDPTAKAEKKEFKPVPPGSY
                     GSAAIILLTDGQRTTGPDSMEAARMAADRGVRVFTVGFGTKGGETIGYEGWSMRVRLD
                     EETLKAIADVTRGQYFYAGTATDLKKVYESLNSKFLLEKKNMEITALFAAAAAITALA
                     SALLSLLWFNRVL"
BASE COUNT          821 a         1219 c         1187 g          857 t
ORIGIN      
        1 tacccacagg ttaattacgc cggctccgcg tccggtcact caggaagata tcaatgccgc
       61 ggtgctgcac acgctcgaga ccaacgttct gccgtccgcg gcggcgaaag cgtatgagat
      121 cattcgaccg tcgatcgtca gagtacgtcg aatggaaccg aacaagaacg gcgaacagga
      181 cgaaaaaggc gtcggcaccg gggtcgtcat cgtcgacaag ggcgtgatct tgaccaatct
      241 tcatgtggta ttcggcgcca agcgggtgca agtcgtgttt gctgatgggc tcgaatccga
      301 agccacggtg accggtatac agcctgagca tgatttggcc gtgcttcagg cgcataagat
      361 tcccgatgat ttgactgcgg ctaccatgcg ctccaccgcg gaccttgcca taggcgatca
      421 agttgtcgcc gtcggctatc cttttggtat aggcccgtcg gcgagtgctg gagtcgtctc
      481 cggattgcgc cgcgagttct attcgccgaa tgggaaacgc atgctgacca atctcatcca
      541 gttcgatgcc gcggctaatc ccggcagctc cggcggaccg ctggtaaccg accggggcga
      601 ggttgtcggg atcgtcactg cgattcttaa tccgaccgcc cagcgttttt ttgtcggcat
      661 tggctttgcg gtacctattg agaatgcggc aaccgctgtc ggtatgtcgc cgttttagcg
      721 taatcgcgcg ccgataaaaa aaggggccta tggacagaga aacaaaccac aaagacgttg
      781 cttcgctcat ggagcgcatc ctctacgagg tgaagaaggt catcgttggc caggatcggt
      841 ttctcgagcg cgtgctggta gcagtactgg cgcaggggca tctgctcgtc gagggcgtgc
      901 ctggtctcgc caagacgctg acggtcaaga cactcgccag aactattcgc ggctcgttca
      961 aacgcattca attcaccccg gatctcgtgc cggcggatct ggtgggcacg cgcatctaca
     1021 atcaaaagac cggcgaattt accactacct tgggaccagt ttttacaaat ttgctgctcg
     1081 ccgatgagat caaccgagcg ccggcgaagg tacaaagcgc gctgctcgaa gtgatgcaag
     1141 agcgccaggt taccatcgcc ggcgagagtc atcgtgtgac cgaaccgttc ttggtgatgg
     1201 ccacgcagaa ccccatcgaa accgaaggaa cctatccgct gcccgaggcg caggtcgacc
     1261 gttttatgat gaaggtgctc gtcggatatc ccaccgagga agaagagttt gtcatcgtcg
     1321 atcgagtgac cggcacctcc aacgatgtcg tgccgctggc caccacggaa caactgtgcg
     1381 cactgcagcg cgagtgtcag cgtgtctacg tcgatccagc cctcaagcag tatgcggtgc
     1441 ggctcgcctc agcgacgcgc gaccccgatc gcttcggggt tgccgaagtg gccaagtacg
     1501 tttctttcgg cgtcagcccg cgcgcctcga ttcatcttgt cgagggcgcg cgggcgctgg
     1561 cgtttctgcg cgggcgcacc tatgttttgt cagaagacgt cacggatata gtgctcgatg
     1621 tatttcgcca ccggctagtg ctgacctatg aagcgctggc cgaagggatg acgccggatc
     1681 acttgatccg gcgtatcatg gagcatatac cagctccgga aaagccgttg gagagccatg
     1741 tgaaacttgg cgcaagcttg taaaattgga gaattgaaat gctgaatatt gccggggtgg
     1801 gttccattat ctcactcggc aactccggta agtcaatgct ctgtcttggg ccaatggagt
     1861 agctgttcaa cggctggaat ttttgaatgg tgtaagcctt ttttcatgct tggagcaatc
     1921 cgcctatgag ttccgtccaa gaatctattc cgacggccgg cgctgaaaac attctgcgcc
     1981 gtctcgagtg gacggtgctc cgacgcctgg acggactgct gcatggcaac tatcagacct
     2041 tgtttcgcgg cttcgggctc gatctcgccg atctgcgcga gtatcagtat tacgacgacg
     2101 ttcgctatat agattggaac gttaccgctc gtctccagac gccttacgtg agggtttaca
     2161 acgaagaccg ggaagtgatg gcctggttcc tactcgacct gagcccgtcc gtggattttg
     2221 gcgcccgagt gaagaagcgt tcggtctcga cggaattcgt caccgtgctg acgcggctcc
     2281 tgactcgtca cggcaatcgc gtcggcgcgc tcttctatgg tgacagtgtc gatacggtaa
     2341 ttccggcgcg cagcggccgg cgtcacgcgc tgcatatctt acacagaatc ctctcccggc
     2401 ccgagacgcc gccctcggcg gcgactaacc tgggcgattt tctccagacc gctctgcgcg
     2461 tcatgaaaag acggtcgctg gttttcatcg tctccgattt catcagcacg cccggctggg
     2521 ccaagccgct cgcgaatctg acgcagcgcc acgaggtcat cgcggtgcgg ctctacgacc
     2581 cgttggagat ggaattaccc gatctcggac tggtcatgat gcgcgacgcc gagaccggcg
     2641 aacaattgtt cgtcgacacc cacgatcgca ctttccgcaa gcgcttcgcg gttttggccg
     2701 agcgccgcga gcaggagctg cgttcggcgt tcacccaggc cggcgtcgac gtgctggagt
     2761 tatcgaccag cgacgatttg gcaaatgcga ttctccggtt cgccgatttg cgcaagcggc
     2821 gcagccagtt ggccgcgggc ggtctgccgc aacatttaag agggagccat gacgtttctc
     2881 tggcctgaaa tgttctggtt gctattggtc gtgccggctc tcgtggccgg ttacttctat
     2941 ctattgcgcc gcaagcagca aaccgcgctc aaatacgcca gtttaagcat ggtaaaagaa
     3001 gcgatggggg ccggccaaag atttcgccgc cacatcccgc cgctgttgtt tttactggca
     3061 ttgatcgcaa tgcttgtcgc cgtcgctcgt cccgccgctg tggtcaccct gccgtcgcaa
     3121 caccaaacca tcatcctcgc catggacgtc tccggcagca tgcgcgccgt cgacgtgcag
     3181 cccaatcgca ttaccgcggc acaagccgcg gccaaagcct tcgtcgccga acagccatcc
     3241 aacgcacgca tcggcgtagt gtcctttgcc ggaacggcgg ccgtcgtgca ggtgcccacg
     3301 cataatcgcg aagacatcat cgccgccatc gatcgctttc aactgcaacg cggcaccgcc
     3361 attggcagcg gcatcatcat ttcgctcgcg accattttcc ccgacgccgg cattgaggtg
     3421 aacgcgtcga actacgggcg ttacgcgcca cgcggcatgg cgctcgaccc gaccgccaaa
     3481 gcggaaaaga aagagttcaa accggtgccg cccggatcct acggctctgc cgcgatcatc
     3541 ttgctcaccg atggccagag gaccacaggc cccgactcca tggaagcggc gcggatggcc
     3601 gccgaccgcg gcgtgcgcgt attcaccgtc ggattcggca cgaaaggcgg cgagaccatc
     3661 ggctatgaag gctggtccat gcgcgtgcgc ctcgacgaag aaacgctcaa agcgatcgcc
     3721 gacgtgaccc gcggccaata tttttacgcc ggcaccgcga ccgatctaaa aaaagtctac
     3781 gagtccctca actccaaatt cttgctcgaa aagaagaata tggaaatcac cgccctcttc
     3841 gccgccgcag cggcaatcac cgcgctggcc tcagcgctgc tctcgctcct atggttcaac
     3901 cgagtcctgt aaagctggag caacacggtt tcgtccagaa gccaacagtg tgaggcgaag
     3961 ggtcgacaac gtcggagctg tcctggcgcg gcatggcagc ttcgccggcc ggaggtatca
     4021 tgttgcatag taaaatgttt gcttgtgcgg tttttctctg gctggcctca gccgattatg
     4081 cctt
//
LOCUS       DASQKS010000241         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_546496, whole genome shotgun sequence.
ACCESSION   DASQKS010000241 DASQKS010000000
VERSION     DASQKS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546496"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VE170_06275"
     CDS             complement(<1..305)
                     /locus_tag="VE170_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HYJ15077.1"
                     /translation="MIRETVSTDKAPKAIGPYAQAIKAGEFIYTAGQIPIDPQSGNLI
                     AGGIAEQTRQVLENLKAVLEAAGSSLEKVVKATVFLKNMADFAALNEVYGEYLGAAKP
                     "
     gene            425..613
                     /locus_tag="VE170_06280"
     CDS             425..613
                     /locus_tag="VE170_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15078.1"
                     /translation="MKLITAHKILITSAVIFFIFFSGWEYRNYAAGDSSAAWRSGLYL
                     IVASGFAVYLKNLKRWYR"
     gene            627..908
                     /locus_tag="VE170_06285"
     CDS             627..908
                     /locus_tag="VE170_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYJ15079.1"
                     /translation="MEPTPFLIEVSATEMRRERQKARELRRSQWWKRKLASGVCHHCA
                     GRFAPAELTMDHLVPVVRGGKSTKGNVVPSCKPCNNQRSHRLPFEKMTE"
     gene            966..1700
                     /locus_tag="VE170_06290"
     CDS             966..1700
                     /locus_tag="VE170_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HYJ15080.1"
                     /translation="MAAETFLVRTMSDTVETDIQRVHVIANELLARYNLPALSFEQLR
                     KIADGPFDLFLIPQIFAAEYAKDRNLPFNETKRVEIRRIALEIARKHGYDVNPPEFQL
                     GIEKSLQETKAAGLRNVLMTTGGRRYKHQAMEKIGIGGYFEEIVDREQTYYAKEQGFY
                     YLYRKQKTPHIRIVLLSGTANYIKAANNADGCKVGDTFLEVIAIALSTQHSYNDEETL
                     RAAAPKAVIYGYDELLPKLKELVPLG"
     gene            complement(1764..2438)
                     /locus_tag="VE170_06295"
     CDS             complement(1764..2438)
                     /locus_tag="VE170_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15081.1"
                     /translation="MDKLTRADAAPELPDAEPRWPAFVATLAAVGLYAALPEVLTMGP
                     PWLFPSVVFALLIPNIVAHHTGSHRLNTLIGFTVDGVLTTGLIIALGLLVTALPSKKV
                     TPIALLLAAASLWVTNILVFALWYWRLDAGGPHQRDKRAAHSEGAFLFPQMTMSEAAL
                     RQTGQRRWSPNFVDYLFLAFNTNTAFSPTDVPVLARWAKVLMMVQSLLSLLIIALLAA
                     RAINLL"
     gene            complement(2603..3865)
                     /locus_tag="VE170_06300"
     CDS             complement(2603..3865)
                     /locus_tag="VE170_06300"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15082.1"
                     /translation="MIPETPDSPDSVAISRIAALHYRDFRLYWVGLFLANIGTWMQIT
                     AVSWLIYRITSSPLQLGMNGFFRAVPAIGLSIISGTIADRYDRKKLMLTTQCLLGALA
                     LLLGVLDHSENIQLWQIYAVTFLSAAVGSCDGPARQALFPTLVPQAVLPNAVALNSVL
                     MKGPALIGPLLAGTVISLIGTHGAFYANAASYLCTVVALLLMRTPTPATAPARKRSFG
                     EDIRSGISYVRSQKIILGIIAMEATTSVFGMDNAMLTIFASDVLRVGAHGFGIMQSAR
                     GIGAVVGSSFFISVGQRPFLGNIILGSSIVYGLCFALFGLSNSYILSLLLLACVGAAD
                     AIWAAARSTILQWTAPDDLRGRVMGIFQIATQGLNPMGQVETGVVVPIIGAREATVFG
                     GSIVWLVTMLTIWRIPQIRKFRLEGSKQ"
BASE COUNT          968 a         1126 c         1137 g          817 t
ORIGIN      
        1 ggcttcgccg caccgaggta ttcgccgtag acttcgttga gcgccgcgaa gtcggccatg
       61 tttttcaaaa acacagtcgc tttgacgacc ttttcgaggg atgaaccggc ggcttccagc
      121 accgctttga gattttccaa aacctggcgc gtttgttcag cgatgccgcc cgcgatgaga
      181 ttgcccgatt gcggatcgat gggaatttgc ccggcggtgt aaatgaattc acccgctttg
      241 atcgcttgcg cgtaaggacc gatggccttg ggcgctttgt cggtgctgac ggtttctcgg
      301 atcattgtca ttctccttgt ctcgcgactt tgacgagcta taacatgaac cccagccggt
      361 gcaacgatcg ctggcctgct cgcttgaact tctcggcccg gctgtgcttg actggcaaga
      421 aacgatgaaa ctcatcactg cccacaaaat attgattacc agcgccgtga tcttctttat
      481 tttcttcagc ggctgggaat atcgcaacta cgccgccggc gactcctcgg ccgcctggcg
      541 cagcgggttg tatctgattg tcgccagcgg attcgctgtc tatctaaaaa acctcaagcg
      601 gtggtaccgc tagaaaattc atggagatgg agcccacgcc ttttctaatc gaagtgtccg
      661 caacggagat gcgccgcgag cggcagaagg cgcgggagct gcgccggagc cagtggtgga
      721 agcgcaagct tgcgagcggg gtttgccacc attgtgcggg tagatttgcc cccgcggagc
      781 tgaccatgga tcatctcgtg ccggtggtgc gcggcggtaa gtcgactaaa ggaaatgtcg
      841 tgccgagctg caaaccatgt aataaccagc gcagccaccg gctgccgttc gagaaaatga
      901 ccgaataaag gctcttgcca cggggtttgg gcttcattac tatcgtttcg ccggaggatt
      961 aagccatggc tgccgaaaca ttcttggtgc gaacgatgtc ggatactgtg gagaccgata
     1021 ttcagcgggt ccacgtgatc gccaacgagc tcttggcgcg ttacaacttg ccggccctga
     1081 gcttcgaaca gctgcgtaaa attgccgatg gacccttcga tctgttcctg atacctcaaa
     1141 tattcgcggc ggaatatgcc aaggatcgca acctgccgtt caatgagacc aagcgggtgg
     1201 aaatccgcag gatcgccttg gagatcgcgc gcaagcatgg ctacgatgtc aatccgccgg
     1261 agtttcaact cggcatcgaa aagtcgttgc aggaaaccaa agccgcgggt ctgcgcaatg
     1321 tgctaatgac caccggcgga cgacgctaca aacatcaagc gatggagaaa atcggcatcg
     1381 gcggctattt cgaagagatc gtcgaccgcg agcaaaccta ctacgccaaa gagcagggat
     1441 tctattacct ctaccgcaaa caaaaaacgc cgcacatacg catcgtcttg ctcagtggca
     1501 cggcgaatta catcaaagcc gccaacaacg ccgatggttg caaagtgggc gacacgtttc
     1561 tcgaagtgat tgccatcgct ttgtcgacgc aacactcata caacgacgaa gaaaccctcc
     1621 gcgccgccgc acctaaagcc gtgatctacg gctacgatga actacttccg aaattgaaag
     1681 agctcgtgcc gctcggttaa atccggcggg cgttgccaac gcgaaaaaac aattcatgtc
     1741 gtttgctcct agtcgctaat ccctcacaat aggttgatag cgcgcgcggc taacagcgcg
     1801 atgatgagca gcgatagcag cgattgcacc atcatcagga cttttgccca gcgcgccaac
     1861 accggcacgt cggtgggcga aaacgccgtg ttggtgttga aagccaggaa caagtaatcg
     1921 acgaaattgg gcgaccagcg ccgttggccg gtttgtctca acgcagcttc gctcatagtc
     1981 atttgaggaa agagaaacgc gccttcgctg tgtgccgcgc gtttgtcccg ttgatgcggc
     2041 ccgccggcat ccagccgcca gtaccaaagt gcaaagacta gaatattggt aacccaaaga
     2101 gatgctgccg caagcagcag cgcgatgggc gtgaccttct tcgacggcag agcggtgact
     2161 aaaagtccga gggctataat gagtccggtc gtgagcactc catcgacggt gaaaccgatg
     2221 agcgtgttaa gccgatggct gccggtgtga tgagcaacga tgttgggaat cagcagcgca
     2281 aaaacgaccg atggaaagag ccaaggcggg cccatggtca agacttcggg taatgccgca
     2341 tagagaccga ccgccgccag tgtggctacg aaagccggcc aacgcggttc agcgtccggt
     2401 aactcgggag ctgcgtcggc gcgagttaac ttatccacaa gactccttcg gcatgaatgc
     2461 gatgatgtga gagatcctag cccgcggcga gtattcaagc gagctgccag aggttgaggg
     2521 attcatcgcg gttgtaaatc cgtacacggc gaagattgcg atcgtttgag ggcgaaaccc
     2581 gaaagctcgc gctaaatagc tctcattgtt tactaccttc gagcctgaac ttgcgaattt
     2641 gcgggatccg ccagattgtc agcatggtga cgagccacac gatcgatcct ccgaaaactg
     2701 tcgcttcgcg cgcgccgatg atcggtacca cgactccggt ttcgacctgg cccattgggt
     2761 tgagcccctg cgtggcaatt tgaaatatac ccatgacccg gccgcgcaaa tcatcgggag
     2821 ccgtccactg cagtatcgtg cttcgcgcag ctgcccaaat tgcatccgcc gctccgacac
     2881 acgcaaggag tagtaacgat aggatgtatg aattcgacaa accgaaaagg gcgaaacaca
     2941 agccgtaaac gatcgaagag cctaatataa tgtttcccag aaatggccgt tggccgacgc
     3001 tgatgaaaaa gctcgagccg accaccgcgc caatgccccg cgccgattgc atgataccga
     3061 agccgtgcgc gccgacacgc agaacatcgc tggcaaaaat agttaacatc gcgttgtcca
     3121 tgccgaagac ggaggtcgtc gcttccatgg cgattatgcc gagaatgatt ttttgtgagc
     3181 gcacgtagct gataccgctt ctgatatcct caccgaagct gcgctttctc gccggcgccg
     3241 tcgccggcgt aggcgtacgc attaataaga gcgccacgac cgtgcaaaga taactcgcgg
     3301 cattcgcata gaaagcgccg tgcgtgccga tcaagctgat gaccgtccct gccagcagtg
     3361 gaccgatcag cgccggccct ttcatcaata ccgaattgag cgccaccgcg ttcggtagaa
     3421 ccgcttgcgg caccagagta ggaaacaacg cttggcgtgc cggtccatcg caggaaccaa
     3481 ccgccgcgct cagaaatgtc acggcataaa tttgccaaag ctgaatgttt tcggaatggt
     3541 ctaagacacc gagtaagagc gccagcgcac cgagcagaca ctgggtggta agcatgagct
     3601 tcttacggtc atagcggtcg gcaatcgtcc cgctgatgat gcttaagccg atcgccggca
     3661 ccgcacgaaa gaacccgttc atgccgagct gaagcggtga gctagtgatt cgataaatca
     3721 gccaactgac cgcggtgatc tgcatccagg taccgatgtt ggcgagaaaa agaccgaccc
     3781 aataaagtcg aaaatcgcgg taatgtaagg ccgcgatgcg actgatcgca acggagtctg
     3841 gactgtctgg ggtttccgga atcacgtgcc acactagcgg ttcacgggaa aattgtcgaa
     3901 gcgttgcgtt aataggccgg cgtaatcagc gagcctcggc atcggcggac tctatacgtt
     3961 gcctgtacca cgcctgacct agatagacga cggtcagagc gtttacggcg aacacggtga
     4021 tcctgaccac cgtcagccgt tcaatcaa
//
LOCUS       DASQKS010000242         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1802228, whole genome shotgun sequence.
ACCESSION   DASQKS010000242 DASQKS010000000
VERSION     DASQKS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802228"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VE170_06305"
     CDS             complement(<1..2056)
                     /locus_tag="VE170_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HYJ15083.1"
                     /translation="MASPQESSPRSSGFSQRLVNRLGADNGRKAAIGLLVLLASAQIA
                     FGEVGWSQARNLWFDAFQQVAPRAVTNLPAVIIDIDDESLQQFGRWPWPRTRLAQLIE
                     ESHRLGALAVGLDMILPEADSQSPQRLLAERQDATAEMRQALDALPSNDAILAKTLRQ
                     VPSVLGRAALIDAVPGKAAPANQTPALIAGESPQRFAWGYNGEIVNLPELESAAHGRG
                     YLNDGRDGDGVVRSMPLVLGVNGAIAPSFVVELLRVATGQAAYSVRSDDKGILGVRIG
                     TSFIPTDRDGRLRLYYSPAQGSRRLSAAAILRGTVAPKALVNQVAIVGATAVGISDVA
                     ATPVGSRMDGVEIHAQLIENILLGARLTRPPAARWWELFGLIAFGIILIVAVPRRRPA
                     IGVALYCAIGATLVMASFVVFLKLQTLYDPTLAVAANGAVAGLILTAGHSAAERRRRE
                     LDAELVVERNERLRAAGELRAAREIQMGMLPDPGSIGGLPANLDFFALLEPAEEVGGD
                     LYDAFMLDDRRLFFMIGDVSGKGVPASLFMALTKTLCKCLARREPADLGQVLSSVNDE
                     ISRENPTAMFVTAIVGVIDGVSGEVELCNAGHNPAILLCTKGLPRELDSAGGPPLCLD
                     CGFTYPTQRLRLADDDMLLLITDGVTEAEDHRQGQFGLARTLQCLAGAPRLSAAGICH
                     KLH"
     gene            complement(2061..>2656)
                     /locus_tag="VE170_06310"
     CDS             complement(2061..>2656)
                     /locus_tag="VE170_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ15084.1"
                     /translation="AYIPGNTIRIHGCHSRALLEEAFLRQQSDPCFPVLWGQLSASIS
                     FVSLEADRWYEIDGLRVRAKLQPHPGTSYGYRFEKAGRSMIYSTDGEHKLKLEEETAA
                     MVEFYRDADLLVFDSMYSFNDMINAKSDWGHSSNIVGVDLCLRAQVKHYCMFHHESDS
                     DDAIYSILGETRRYAEIAGENHSLQVSTAYDGLVIDL"
BASE COUNT          573 a          846 c          757 g          480 t
ORIGIN      
        1 catgaagctt atgacaaatc cctgccgcgc ttagccgcgg cgcgccggcc aagcactgaa
       61 gcgtacgcgc caatccaaat tgtccttgcc ggtgatcttc cgcctcggta actccgtcgg
      121 tgattaacag cagcatatcg tcatcggcca aacgcagtcg ctgcgtcggg taggtaaaac
      181 cgcaatccag gcaaagcggc ggtccgccgg cactgtcgag ttcccgtggc aacccttttg
      241 tacacaacag gatcgcgggg ttatgaccgg cgttacacaa ctcaacctct cccgaaacac
      301 catcgatgac accgacgatc gccgtgacga acatcgccgt cggattttcc cgcgagattt
      361 catcatttac cgaacttagc acttggccga ggtcggctgg ctccctccgc gccaggcatt
      421 tgcacagggt cttggtcagc gccatgaaca aagatgccgg cacacccttg ccggaaacgt
      481 cgccgatcat aaaaaacagc cgccgatcgt cgagcatgaa ggcgtcgtag agatcgccgc
      541 cgacttcttc cgccggttct aacaacgcga agaaatcgag attcgccggc agtccaccga
      601 tcgatccagg atcgggcagc atgcccattt gaatttcccg cgccgcgcgc aattcgccgg
      661 cggcgcgcag ccgctcgttg cgctcgacga cgagctcggc atcgagctcc cgccggcggc
      721 gttcagccgc gctatggccg gcagtcaata tcaagcccgc caccgcgcca ttggccgcca
      781 ctgcaagcgt cggatcgtaa agggtctgca gcttgagaaa cacgacaaag ctcgccatca
      841 ccagggtggc tccgatagcg caatataaag cgacgccgat cgccggccgg cgccgcggca
      901 cggcaacaat cagaataatg ccgaaggcaa tgagaccgaa caattcccac caacgcgccg
      961 ccggcgggcg cgttaaacgc gcaccgagca gaatattctc gattagttgg gcgtggatct
     1021 cgacgccgtc catgcgtgaa ccgaccggag tcgccgccac gtcgctgatg cccaccgccg
     1081 tggcgccgac gatcgccact tgattgacca gcgccttggg cgccacggtg ccacgcagga
     1141 tcgccgccgc cgagagccgc cgtgaacctt gtgcgggcga atagtacaga cgcaaccggc
     1201 cgtcacgatc ggtgggaata aacgacgtgc cgatccgcac gccgaggatg cccttgtcat
     1261 cgctgcgcac cgaataggcc gcctggccgg tggccacgcg cagtagctcg acgacaaacg
     1321 acggtgcaat ggcgccgttg acgccgagca ccaaaggcat gctgcggaca acgccgtcgc
     1381 cgtcgcggcc gtcgtttaaa tagccgcggc catgggcagc agattcaagc tccggcaaat
     1441 taacgatctc tccgttataa ccccatgcga accgctgcgg cgattctccg gcgatcagcg
     1501 cgggagtttg gttcgctggt gcagctttcc caggcacggc gtcgatcagc gccgcccgtc
     1561 cgagcacgga cgggacttga cgcagagtct tggcgagaat tgcatcgttg gacggcagcg
     1621 cgtcgagcgc ttggcgcatt tccgcggtgg catcctgacg ctcggccagc aagcgctgcg
     1681 gcgactggct atcggcctcg ggcaaaatca tatctaaacc caccgccagt gctcccaacc
     1741 gatggctctc ctcgattagc tgcgccagac gcgtgcgtgg ccagggccag cggccaaatt
     1801 gttgcaggct ttcatcgtcg atatcgatga tcaccgccgg aagattagtc actgcgcggg
     1861 gcgccacttg ttgaaaggcg tcaaaccata aattccgcgc ctggctccag cccacttcac
     1921 cgaaggcgat ctgcgccgac gccaacaaca ccaatagccc gatcgccgcc ttgcggccgt
     1981 tatcagcgcc caaacgattg accagccgct ggctgaaccc actgctgcgg ggtgaggatt
     2041 cttgaggcga cgccacgaag ttagagatcg atgactaagc cgtcgtaggc tgtcgagact
     2101 tgaagagaat ggttttcacc ggcgatctcg gcgtagcgcc gggtttcgcc gaggatagaa
     2161 taaattgcat cgtcgctgtc cgactcatgg tggaacatac aataatgctt aacctgggcg
     2221 cgcaaacata gatcaacgcc gacgatattg ctcgaatggc cccagtctga ttttgcgttg
     2281 atcatgtcat tgaacgaata catcgaatcg aacacgagca aatcggcgtc gcggtaaaat
     2341 tccaccatcg ccgcggtctc ctcttccaac ttgagtttgt gctctccgtc ggtcgaatag
     2401 atcatgctcc ggccagcttt ttcgaagcga tagccgtatg aagtgccggg atggggttgc
     2461 agcttcgccc tgacgcgcag accgtcgatc tcgtaccaac gatccgcctc cagcgaaaca
     2521 aagctgatgc tcgcgctcaa ttgaccccag agcaccggaa aacagggatc ggattgctgg
     2581 cgcagaaacg cctcttccaa caacgcccgc gaatggcagc catggatgcg gattgtgttg
     2641 cccggaatgt aggccg
//
LOCUS       DASQKS010000243         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_547499, whole genome shotgun sequence.
ACCESSION   DASQKS010000243 DASQKS010000000
VERSION     DASQKS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547499"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VE170_06315"
     CDS             <1..729
                     /locus_tag="VE170_06315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ15085.1"
                     /translation="LSGTDAADGDGRIAPPSSIQSQTTEVLTRIGRILAGHELGLGNL
                     CRTFMFMPGTRYRPGYGEARKKVYQGVFTEDAYPPNSGIYIRSLGENILLRSMAIAYR
                     GEHKIVTSPQVRLAPGSFSQSVRVGDWLLLAGQDAVTFDRVVECEGSLAGQTEATLRH
                     TKDIVEAAGGSLDDIVKTTVYLVGGTDRAEFAEAYRSFFAGHSRSRTMPAGLTVVVDE
                     LSPRCLVEIDAVAYLPSQITRQLK"
     gene            complement(734..1105)
                     /locus_tag="VE170_06320"
     CDS             complement(734..1105)
                     /locus_tag="VE170_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15086.1"
                     /translation="MTLEQAVISYSVVLVVLLALMVWMLMRHWAVKRRKAQAANPGAG
                     AVEQTASPPMDWRGTISKLRPDNVLTGEMWVKAVIAAFIALCAGTIVVVFVFPLGAAA
                     AAIASFAAVVVIVGLYVILLD"
     gene            complement(1266..2273)
                     /locus_tag="VE170_06325"
     CDS             complement(1266..2273)
                     /locus_tag="VE170_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15087.1"
                     /translation="MIMRLSHGAAVWVSALLILVIANAGAQTGKPEKKSLEIAVAAFA
                     HTTMPLLIAHEAGYFAKYGLGVNISAVSAGVAVQGLISNRIDIYQGGSAAVMATLGGA
                     DIIYVAAGVDKSSLMLFGQRGVTSFDVLRGKTVATTSPGAFGEIAMRGTARKYGMEIP
                     KDIKLMFHGTPAQAYSTFTLGNSDAMINTPPQSDMAKQKGYPVIIDFYKEGLKIIGPG
                     TAVTREFAQKNPNTIRAFLMGLLDGVKRAIDDEEYASKLESKYSKVTDVKILADNYQQ
                     GLRVWNKDLTVDPAAIRVVLDDAPDGRGKGVDPKRFYDNSFIQSVNRDHASKLFPGEV
                     K"
     gene            complement(2338..>2811)
                     /locus_tag="VE170_06330"
     CDS             complement(2338..>2811)
                     /locus_tag="VE170_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15088.1"
                     /translation="LLGNAEGLVITPPQTDLAKQKGYPMIVDYFKEGFKIIGPGTSLT
                     REFAQKYPNTVKAYLMAYLDGLRRTIDDEEYAKKICAKYTKLTDINILTETYQQGLRV
                     WNRDMTVDPAAIRNVLDDSSDAKAKGADPKRFYDNSLMQQVNRDHAAKLFPGEIR"
BASE COUNT          575 a          858 c          752 g          626 t
ORIGIN      
        1 ctttccggca ctgacgccgc ggacggcgat ggacggatcg ccccgccttc gtcgattcaa
       61 agccaaacca ccgaagtcct cacgcgtatc gggaggattc tcgccggaca tgaactcggc
      121 ctcggcaatc tctgccgcac cttcatgttc atgcccggca cccggtaccg tcccggctac
      181 ggcgaggcgc gcaagaaggt ttatcaaggc gtttttaccg aggatgctta cccgcccaac
      241 tccggcatct acattcgcag cctcggcgaa aacattttgc tgcgctccat ggccatcgcc
      301 taccgcggtg aacataagat cgtcaccagt ccccaggtgc gcctggcgcc cggttctttc
      361 tcgcaatcgg tgcgggttgg cgattggctg ctgctcgcgg gtcaagatgc cgtaacgttc
      421 gaccgggtgg tagaatgtga aggcagtctc gccggccaaa ccgaggcgac attgcgccac
      481 accaaagaca tcgttgaagc cgccggcggc agcctcgacg acatcgtcaa gaccactgtg
      541 tatttagtcg gaggaactga tcgcgcggaa ttcgccgaag cctatcgaag tttcttcgcc
      601 ggccacagcc gctcgcgcac catgcccgct ggcttaaccg tagtcgtcga tgagctatcg
      661 ccgcggtgct tggtggagat cgatgcggta gcttacctgc cgagccaaat cactcggcag
      721 ctaaaataga agattagtcc agcaggatta catataggcc gacgatgacg accaccgcgg
      781 caaaagacgc gatcgccgcc gccgcggcgc cgaggggaaa aacgaacacc acaacgatgg
      841 tgccggcgca aagagcgata aaggcagcga tgaccgcctt cacccacatc tcaccggtaa
      901 gaacgttgtc cggccgcaat ttacttatag tgccgcgcca atccatcggc ggcgatgccg
      961 tttgctcgac cgcgccggcg cctgggttgg ccgcttgggc tttgcgccgc ttcaccgccc
     1021 agtgccgcat tagcatccag accattagcg ccagcagcac aacgagaacc actgaataag
     1081 atattaccgc ctgctcaaga gtcatcgcgc tcgttccacc ttcacagacc cggctctgtg
     1141 accgtttccc tcatggcaaa tattaagcga tcaacggtcc attgttccaa cgccggtggc
     1201 gggattttgc tctttcgagt gcatttaacg acaacgattg ccagaattac cactgacgtt
     1261 gccgcttatt tcacttcgcc cggaaacagc ttcgacgcat ggtcgcggtt gactgattga
     1321 ataaacgaat tgtcgtagaa tcgtttggga tcgaccccct tgcctcggcc atcgggcgca
     1381 tcgtcgagca cgacccggat cgccgccgga tcgacggtca gatccttgtt ccagacgcgc
     1441 aatccttgtt ggtagttatc agcgaggatt ttgacgtcgg tgactttgga atatttcgat
     1501 tcgagcttgc tggcatactc ttcgtcgtcg atggcgcgtt tcacaccgtc caatagtccc
     1561 atcaaaaatg ctctgatggt gttgggattt ttttgcgcga actcccgcgt caccgcggtg
     1621 ccagggccaa tgattttcaa tccttccttg taaaaatcga tgattaccgg atagcccttt
     1681 tgcttggcca tgtccgactg cggcggcgtg ttgatcatgg cgtcggagtt gcccaaggta
     1741 aacgtcgaat acgcctgcgc cggcgttccg tgaaacatta acttgatgtc cttgggaatc
     1801 tccataccgt acttccgtgc cgtcccgcgc atggcgatct cgccgaaagc gccgggcgag
     1861 gtcgtcgcca ccgtcttgcc gcgcaaaaca tcgaagctcg tgacgcccct ctggccgaac
     1921 agcatgagcg aactcttgtc gacgccggcg gcgacataaa taatatccgc gccgccgaga
     1981 gtcgccatca ccgccgccga cccaccctgg taaatatcga tcctgttcga gatcaatccc
     2041 tgcaccgcga cccctgcgct gaccgcgctg atgttcacgc cgagtccgta tttcgcgaaa
     2101 tagcccgcct catgggcgat caaaagcggc atcgtcgtat gagcgaacgc cgccacggcg
     2161 atttcgagac tcttcttttc cggcttgccg gtctgcgcgc cggcattggc aatcaccagt
     2221 atcagcagcg ccgacaccca aacagccgca ccatgtgaaa gtctcataat cataagccgt
     2281 ttaacctcct gcgatactca ccggttgtgc ctgcgaaact cctggctact tcctgatcta
     2341 gcgaatttct ccgggaaaca gcttggcggc atgatcgcgg ttgacctgtt gcatcagcga
     2401 gttgtcgtaa aaccgctttg ggtcggctcc cttggctttg gcatcggacg agtcgtcgag
     2461 cacgttgcgg atcgccgccg ggtcgacggt catatcgcgg ttccatacgc gcagcccttg
     2521 ctgataggtt tcggtcaaaa tgtttatatc cgtcaacttg gtatatttcg cgcagatctt
     2581 cttggcatac tcctcgtcgt cgatggtccg ccgcaggccg tcgagatagg ccatcaaata
     2641 tgccttgacg gtattaggat atttctgcgc gaattcgcgc gtcaatgagg ttcccggtcc
     2701 gatgatttta aagccctctt tgaaataatc gacgatcata ggatagcctt tttgcttggc
     2761 caaatcggtc tgcggcggcg tgatcaccaa cccttcggcg ttgccgagga g
//
LOCUS       DASQKS010000244         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_122256, whole genome shotgun sequence.
ACCESSION   DASQKS010000244 DASQKS010000000
VERSION     DASQKS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122256"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VE170_06335"
     CDS             complement(<1..738)
                     /locus_tag="VE170_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15089.1"
                     /translation="MKRTILFVAAFLLCITGQAAVITNVTISAVSSEFVSGIDQRRAT
                     NIVNGVGLFGDVHTRIPQGAMWLTLPDTAGAAAQAFVEFDLGATHTLNQMKVWNYNEG
                     PSGANILTRRGVQTADIQIAGDDKQFTTTKAAQTFRRATGAFTNFFDVIDMGGVQARY
                     VRINVLTNFGEGDNRVGLSEVQFIENTAAPALRTAERSFSGDRVTLQFSEAMDPASAT
                     NTANYAITGPSGVVTISSADFHIYKDAV"
     gene            complement(1026..>2704)
                     /locus_tag="VE170_06340"
     CDS             complement(1026..>2704)
                     /locus_tag="VE170_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HYJ15090.1"
                     /translation="ALKFAADMVKEKLIDWETAIMRNPADQLEQLLAPVFDLNDVKKS
                     KVIATGLPAGPGAATGKIYFNADRAVEAAEKGEKVLLVRVETSPEDLRGMIAAEGILT
                     ARGGVSSHAALVARQMGKVCVCGAAALQVNYDSKTLVVGDVTYKEGDFLSIDGTAGTV
                     YGGQIKTAPSEIIAGLINGDKQAQATEKFKSYVQLMKWCAQASRLQIRTNADNPEQTR
                     NAVAFGATGIGLTRTEHMFFEGDRIDAMREMILADSLEAREAALAKLLPYQREDFFGI
                     FKELKGFPATIRFLDPPLHEFLPHGKEQQMDLARKLGIPYEKIATRVNELHEFNPMLG
                     FRGCRLGIKYPEITRMQSRAVFEAAADATKKGFKAKPEIMIPLVGFKRELDLQVAIVH
                     ETAKAVQAEKKVKFAYSVGTMIEVPRGALTANEIAETAEFFSFGTNDLTQTALGMSRD
                     DSGSFLAPYQEAEIMKRNPFATIDQTGVGQLMQIAIEKGRQTRPDIKLGICGEHGGDP
                     DSVKFCHKIGLTYVSCSPYRVPVARLAAAQAAIEEKRNAAKAVKPAAKKR"
BASE COUNT          517 a          781 c          824 g          582 t
ORIGIN      
        1 aacggcatcc ttgtagatgt ggaaatcagc ggacgagatt gtgacgacgc cgctcggacc
       61 agtgatcgcg taattggcgg tgttggtcgc gcttgccggg tccatggcct cggagaattg
      121 aagcgtcacc cggtcgcccg agaagctccg ctcggcggtc cgcaaagctg gagccgccgt
      181 gttctcgata aattgaacct cgctcaagcc gacgcggtta tcgccctcgc cgaaattggt
      241 gagcacatta atgcgcacgt agcgggcttg aacaccgccc atgtcgatta catcgaaaaa
      301 gttcgtgaaa gcgccggttg cgcggcggaa ggtctgtgcg gcttttgtgg tggtgaactg
      361 cttgtcgtcg ccggcgattt ggatgtcagc cgtttgcacg ccgcggcgcg tcaggatgtt
      421 tgcgccgctc gggccttcgt tatagttcca gaccttcatc tggttcagcg tgtgcgtcgc
      481 tcccaagtcg aactccacga acgcctgcgc cgcagcgccc gccgtgtcgg gtaaagtaag
      541 ccacatcgcg ccctgcggaa tgcgcgtatg cacgtcgcca aaaaggccga cgccattcac
      601 aatgttggtg gcgcgacgtt gatcgatgcc ggacacaaac tccgacgaga ccgcgctaat
      661 ggtaacgttg gtgataaccg ccgcctggcc tgtgatgcac agcaaaaaag ctgcgacgaa
      721 aagaatggta cgcttcattg gggacctcct atggctgatt gaggcgccag ttgtagatcg
      781 catgccgatg cacggcaaca aaaaagggcg cggttctgct ctttgtcgcc aaagtaaatc
      841 tccacctgat caagaaagtt ctgcgcgggc cggctgatct tgtcgccgaa gtgatttcac
      901 ccagttcgca caaccgttat cgcattcaaa agcgcgatct gtatgagcag catgggctca
      961 aggaatattt aaaaaaaagc cgccctacct tcgcaggcga gcggctcgtg tcagggcaga
     1021 tttttttagc gtttcttggc ggcgggcttt acggctttgg cggcatttcg cttctcctcg
     1081 atcgcggcct gggcggcggc gaggcgcgcc accggcacgc gatacggcga gcagctcacg
     1141 taggtgaggc cgatcttgtg gcagaacttc acggaatccg gatcgccgcc gtgttcgccg
     1201 cagatgccca gtttgatgtc gggacgcgtc tggcggccct tctcgattgc gatctgcatc
     1261 aactggccga cgccggtctg gtcgatggtc gcaaacggat tgcgcttcat tatttccgct
     1321 tcctggtagg gagccaagaa cgagccggaa tcgtcgcggc tcatgccgag cgcggtctgc
     1381 gtaaggtcgt tcgtgccgaa gctgaaaaac tcagcggttt cggcgatctc atttgccgtc
     1441 aaggcgccgc gcggaacttc gatcatcgtg ccgacgctgt aggcgaattt caccttcttc
     1501 tcggcctgaa ccgccttggc cgtttcatga acgatcgcga cctgcaggtc gagttcgcgc
     1561 ttgaagccaa cgagcgggat catgatttca ggcttggctt tgaagccctt cttggtcgca
     1621 tcagcagccg cttcgaacac ggcgcgggat tgcatgcggg tgatttccgg atacttgatg
     1681 ccgaggcggc aaccgcggaa accaagcatc gggttgaact cgtgcagttc gttgacccgg
     1741 gtcgcaatct tctcgtaagg aatgcccagc ttgcgcgcga gatccatctg ctgctccttg
     1801 ccgtgcggca ggaattcgtg caacggcgga tcgaggaaac gaatcgtcgc cgggaaacct
     1861 ttcagttcct tgaagatgcc gaagaaatct tcgcgctgat acggcagcaa cttagccagc
     1921 gcagcttcgc gggcttcgag cgagtcggcg agaatcatct cgcgcatggc gtcaatgcga
     1981 tcgccttcga agaacatgtg ctcggtgcgg gtgaggccga tgcccgtcgc gccgaaggcc
     2041 acggcgttgc gagtttgctc cgggttgtcg gcattggtgc gaatttggag gcgcgaagcc
     2101 tgcgcgcacc atttcatcaa ctgcacgtag ctcttgaatt tctcggtcgc ctgcgcctgc
     2161 ttgtcgccat tgatcaggcc cgcgatgatt tccgaaggag cggttttgat ctggccgccg
     2221 taaaccgtgc cggccgtgcc gtcgatggag aggaagtcgc cttccttgta cgtcacgtca
     2281 ccgacgacca gcgtcttgga gtcgtaattg acctgcaaag cggcggcgcc gcaaacgcag
     2341 accttcccca tctggcgagc tacgagcgcc gcgtgcgaac tgacgccgcc gcgcgcagtc
     2401 aaaatgcctt ccgccgcgat catgccgcgc aaatcttcgg gcgacgtttc aacgcgcacg
     2461 agcagaacct tttcgccctt ttccgcggct tcgacagcgc gatcagcgtt gaagtaaatc
     2521 ttgcccgtgg cggcgccagg accggccgga agaccggtgg caatcacctt ggactttttg
     2581 acgtcgttca aatcgaaaac gggcgcgagc aactgttcga gctgatcggc cgggttgcgc
     2641 atgatggcgg tttcccagtc gatgagtttc tctttcacca tgtcggcggc gaacttgagc
     2701 gcgg
//
LOCUS       DASQKS010000245         5484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_549949, whole genome shotgun sequence.
ACCESSION   DASQKS010000245 DASQKS010000000
VERSION     DASQKS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549949"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="VE170_06345"
     CDS             <1..1453
                     /locus_tag="VE170_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ15091.1"
                     /translation="ADASTSRKYGGTGLGLAISRELATLLGGEIRLNSVLGQGSAFTL
                     YLPMTYSEPVASGLLRRGGFADPVSVPALSAVAATAEIIPDDRELIVEGDPVLLIIED
                     DPHYARVLLGLARDKGFKGLVATRGNIGLDLAREYKPAAITLDIFLPDMLGWTVLNNL
                     KRDSATRHIPVQIISLEEEQRHGLAHGAFSYMVKPVTTEGIEQCLERIHSFITPRVKR
                     LLVIEDNQVESQSIVELLAHDDIEIVVAGTGSGALEALAGSAFDCCVLDLRLPDMSGF
                     ELLERIKTDPRLQHLPVVVFTGKDLGSEEENSLKSMAKSIVLKDVQSPERLFDETALF
                     LHRIIDQLPPAKQRMLERLHSSNEVLHGRKVLVVDDDARNIFALSTILENHDMQVMSA
                     TNGRQAIELLQTTPDVSVVLMDIMMPEMDGYETMREIRKRGDFHSTPILALTAKAMKG
                     DREKCLEAGASDYVAKPVNTEQLLSMLRVWLYR"
     gene            1522..2637
                     /locus_tag="VE170_06350"
     CDS             1522..2637
                     /locus_tag="VE170_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HYJ15092.1"
                     /translation="MSEPTNILLVDDQARNLDSLEAILSSPDYHLFRAQSANDALLAL
                     LAHDFAVIVLDIRMPEVSGLELAQLIKQRKKNQHLPIIFLTAYLQDDKDILEGYGAGA
                     VDYLSKPINPLILKSKVAVFVELYRKTCELARLNEQMESEIEERVAQIRASLQEKEIL
                     LKEVHHRVKNNMQIISSLLSLQAERANDGPTQMNFRDLRDRVHSMALVHEKLYQSENL
                     ARVQIADYTRSLIGDLYQAYASGMPKVQLHLDLESAQMPVDRAISFGLILNELATNAF
                     KHAFRGRDNGEIRITLQTEPAGVIRLRFADDGRGFDEPLLWESVDSLGLRLVRMLTRQ
                     LGGQLEMRRERGTTFELSLKNDPEARAPGNNEEAGSL"
     gene            2634..4619
                     /locus_tag="VE170_06355"
     CDS             2634..4619
                     /locus_tag="VE170_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ15093.1"
                     /translation="MSRSILVVEDEAIVALDLSQRLTRLGYRVVDTVSEGEKALAAVL
                     EHSPDLVLMDLRLAGAQNGIATAQQIALVSDVPVVYLTAHSDSETLAQACLTGPFGYI
                     LKPFDERELHTQLEIALYRHGAERRVRESEARLRSIMDTAADCIMEIDDNGTLLNYNP
                     AAEQIFGFAAGEMAGGNACILMPDFSRILSKDENQWSLKPGGGNVLGEVQELNAIRKD
                     GAVIPVELTVSRKDGPQKRTYIGIVRNISRRKQLENKLKQLVDDLRETDRRKDEFLAT
                     LSHELRNPLAPIRNAATILKRVDPADDKFQWCCDILDAQVEHMSRLLEDLLDVSRIAR
                     GSLLLRQEWLDFREIMSHIVAACRPDVEAAGHQLVVDLPDKEVILFADRARLVQIFGN
                     LLTNAAKFMDGNGVVRFTATSENQAIVVQVQDTGIGIAPDFLPHVFEMFKQADSSVER
                     SRCGLGIGLALVKSLVELHGGTVEAQSVGRNQGSVFTVRLPITAAPANVKTPANVAPG
                     SDTARALIRGKKVLIVDDNKLQAQSLGMLLRLWGHETHLVYDGAAALASLNEFPADVA
                     LIDIGLPDMNGDEVAGQIRRQPQWRRMTLIAQTGWGRDSDRVKTAQAGFDHHLTKPLD
                     HQLLEKILNQTATQNLDSSTDFADFQHLHPISPMKPG"
     gene            4876..>5484
                     /locus_tag="VE170_06360"
     CDS             4876..>5484
                     /locus_tag="VE170_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15094.1"
                     /translation="MVWKRIGNFKLSCGLIVAGAVMMSCYLTPKIPIVADEQIERMVS
                     DEAGRILAAADPRAEFSHYRFQLSAFPRQDLLGVSIGRGQIYISYQLAQLALKKSYYR
                     WLLRQTLAHEIAHELAGHANQRGAVANSPASQGVTARHLGLGGLVQFQNYSVEKELQA
                     DFYAMNYWSALGWDCAIWVDILRGFQKQNYAGDVFHPTDRRLE"
BASE COUNT         1194 a         1571 c         1613 g         1106 t
ORIGIN      
        1 agccgacgcc agcaccagcc gtaaatacgg cggcaccggc ttaggtctgg cgatcagccg
       61 cgaactggcg actttgctcg gcggggagat ccgtctaaat agtgttttgg gccagggcag
      121 cgcttttacg ctttatttgc cgatgactta cagcgagccc gtcgccagcg gcctgttgcg
      181 ccggggcggc tttgccgatc cggtcagcgt cccggcgctg tcggcggtgg cagccactgc
      241 cgagatcatt cccgacgatc gcgagctcat cgtcgagggc gatccggtgc tgctgatcat
      301 cgaagacgat ccgcactatg cccgcgtgct gctcggcctg gcgcgcgaca aaggcttcaa
      361 aggattagtc gcaacccgcg gcaacatcgg cctcgacctg gcgcgtgaat acaagccggc
      421 ggcgatcacg ctcgatattt tcctgcccga tatgctcggc tggacggtgc tcaataattt
      481 gaagcgcgat tccgcgacgc gccatattcc cgtgcagatc atctccctcg aagaagagca
      541 gcgccacggt ctggcgcacg gcgccttctc gtacatggtc aagccagtga ccaccgaagg
      601 catcgagcaa tgtctggagc gcattcacag ctttatcacg ccaagggtca agcgcctgct
      661 ggtgatcgaa gacaatcagg tcgaaagtca gagcatagtc gaactcttgg cccacgacga
      721 tatcgagatc gtcgtcgccg gcaccggctc gggggcgctc gaggcattgg ctggcagcgc
      781 gtttgactgt tgcgtgctcg atctgcgctt gccggatatg agcggatttg aattgctcga
      841 acggatcaaa accgatcccc ggctgcagca tttgccggtg gtggtcttca ccggcaagga
      901 tctcggttcc gaggaagaga acagccttaa atccatggcg aagagcatcg tcctcaaaga
      961 cgtgcaatcg cccgagcggt tgtttgacga aaccgctttg ttcctgcacc ggatcatcga
     1021 ccaattgccg ccggccaagc aaaggatgct cgagcggctg cattcgtcta acgaggtgct
     1081 gcacggccgc aaggtgctcg tggtcgacga cgacgcgcgc aatattttcg ccctgtcgac
     1141 gattttggaa aaccacgata tgcaagtcat gagcgccacc aacggccgcc aggcgatcga
     1201 gttgttgcaa acaacgcccg acgtcagcgt ggtgttgatg gacatcatga tgccggaaat
     1261 ggacggctac gaaaccatgc gcgagatccg caagcgcggc gatttccact cgacgccgat
     1321 cctcgcgctc accgccaagg cgatgaaggg cgaccgggaa aaatgtctcg aagccggcgc
     1381 ctcggattac gtcgccaagc cggtcaatac cgaacagctc ttgtcgatgt tgcgggtgtg
     1441 gctgtatcgg taaatggaac ggaccgacga cagcgaaccg taagggcgat tcatgaatcg
     1501 cccctacatt gatcttatgc tatgagcgaa ccgaccaaca ttctgcttgt cgacgatcag
     1561 gcgcgcaatc tcgattcgct cgaggcgata ctgagttcgc cggactatca tctgttccgc
     1621 gcgcaatcgg ccaatgatgc gctgttggcg ctgcttgccc acgacttcgc ggtcatcgtg
     1681 ctcgatatcc gcatgccgga ggtgagcggt ctcgagctcg cgcagttgat caagcagcgc
     1741 aaaaaaaatc agcatctgcc gatcattttt ttgactgcct atttgcagga cgacaaagac
     1801 attctcgaag gctacggcgc cggcgcggtg gactatttga gcaagccgat caacccgttg
     1861 attctgaaat ccaaggtcgc cgtctttgtc gagctgtatc gcaaaacctg cgagctggcg
     1921 cgtctgaacg aacaaatgga gagtgaaatc gaagagcggg tggcgcagat ccgtgcgagc
     1981 ttgcaagaga aggaaatatt gctcaaggaa gtccatcacc gagtaaaaaa caacatgcag
     2041 atcatctcga gcctgctgag cctgcaagcc gagcgcgcca acgatggccc cactcagatg
     2101 aattttcgcg atttgcgcga tcgcgtgcac tcgatggcgc tggtgcacga gaagctctac
     2161 cagtcggaga atttagcgcg tgtgcagatt gccgactaca cgcgcagttt gatcggcgat
     2221 ctgtatcaag cctacgccag cggcatgccc aaggtgcaac tccacctcga tctcgagagc
     2281 gcgcagatgc cggtggatcg cgccatctcc tttggattga tcctgaacga gttggcgacc
     2341 aatgccttca agcatgcatt tcgcggccga gacaatggcg agatccggat cacactgcaa
     2401 accgagccag ccggcgtaat ccgccttcgt ttcgccgacg atggccgggg ctttgatgag
     2461 ccgctgttgt gggagagcgt cgattcactc ggcctgcgtt tagtgcgcat gctgacccgg
     2521 cagctgggcg ggcaacttga aatgcgtcgc gaacggggca cgaccttcga gctaagcctt
     2581 aaaaacgatc ctgaggcaag agcgccgggc aacaacgagg aagcaggtag cctatgagcc
     2641 gtagcattct ggttgtcgaa gatgaagcga tcgtcgcgct agatttgagc cagcgattga
     2701 cccgcctggg ctatcgagtg gtcgacaccg tcagcgaagg cgaaaaggcg ctggccgcgg
     2761 tgcttgaaca tagtcctgat ctggtgttga tggacctgcg actggccgga gctcaaaatg
     2821 gcatcgccac agcacagcag atcgccctgg tgagcgacgt cccggtcgtc tatttgaccg
     2881 cccattccga ctccgaaacc cttgcccaag cctgtttaac cggccccttc ggctatattt
     2941 tgaagccgtt cgatgagcgc gaactgcaca cccagctcga aatcgccctc taccgccacg
     3001 gcgccgaacg gcgcgtgcgc gagagcgaag ctcggttacg atcgatcatg gacacggcgg
     3061 ccgattgcat catggaaatc gacgataacg gcacgctgct taactataat ccggccgccg
     3121 aacaaatatt cggtttcgcc gcgggtgaaa tggccggcgg caacgcctgc atcttgatgc
     3181 cggatttttc ccgcatcctg agcaaggacg aaaaccagtg gtcgcttaaa cccggcggtg
     3241 gaaatgttct tggcgaggta caggagctta acgccatccg aaaggacggg gcggtgattc
     3301 cagtcgagct gacggtgagc cgcaaagacg gtccccaaaa gcgcacctac atcggcattg
     3361 ttcgcaacat ctcccggcgc aagcaactgg aaaacaaact caaacagctg gttgacgatc
     3421 tgcgcgagac cgatcggcgc aaggacgaat ttttggcgac gttgtcgcac gaactgcgca
     3481 accccctcgc gccgatccgc aatgccgcga ctattttgaa gagggtcgat ccggccgacg
     3541 acaaatttca atggtgctgc gatatcctag acgcccaagt cgagcacatg agccgcctgt
     3601 tggaagatct gcttgacgta agccggatcg cccgcggcag ccttttgctg cgccaagaat
     3661 ggctcgattt tcgcgaaatc atgagccata tcgtcgccgc gtgccgcccc gatgtcgaag
     3721 ccgcggggca tcagctggtg gttgacctgc cggacaagga ggtcattctg tttgccgacc
     3781 gagcccgact ggtgcaaatc ttcggtaacc tgttgaccaa tgccgcaaaa ttcatggacg
     3841 gcaacggcgt ggttcggttc accgcgacaa gtgaaaatca agcgatcgtc gttcaagtcc
     3901 aagacaccgg catcggcatc gcgcccgatt tcttgcccca tgtgttcgaa atgttcaagc
     3961 aggccgatag cagtgtggaa cgcagccggt gcgggctcgg catcgggctc gccttggtga
     4021 aaagcctggt ggagcttcat ggcggcacgg tcgaggccca gagcgtgggg cggaatcaag
     4081 gcagcgtgtt taccgtgcgt ttgccgataa ccgcggcgcc cgccaatgtt aaaaccccgg
     4141 cgaacgtcgc gcccggcagc gacacagcgc gggcgctaat acgcggcaaa aaggtgctga
     4201 ttgtcgatga caacaagctc caagcacaga gcctgggaat gttgctgcgg ctgtggggcc
     4261 atgaaaccca cctggtttat gacggcgcgg cagcgctcgc ttcgttgaac gaattccctg
     4321 ccgacgtggc gcttatcgat atcggattgc cggatatgaa cggcgacgaa gttgccggac
     4381 aaatccgccg gcaaccgcaa tggcggcgca tgacgctgat cgcgcagacc ggctggggcc
     4441 gcgacagcga tcgtgtgaaa accgcgcaag ccggcttcga tcatcatctt accaagcccc
     4501 tcgaccacca gctgttggaa aagattctca atcagacggc gacgcaaaat ctcgattcat
     4561 cgacagattt cgcggatttt cagcatctcc atccgatcag tccgatgaaa ccgggttgaa
     4621 cggttaaggg actgatcgcg acgccaccta agattcacaa aatccccaat ctcgccgggg
     4681 cggcatgaac taacgcgagt tttttaccgc ctggggtcct ggccagatta gtccatggca
     4741 ggctgggcgt ttgccgcccc gacgtgtcag cgggtttcgg ttttaggaaa agggttggcg
     4801 gcgcctttgc gctatgctag tgtgcggcag ttttcacctc cgatgactca cgattcgggc
     4861 cttaagcagg agagcatggt ctggaaaaga attggcaatt ttaaattgtc gtgtggcttg
     4921 attgtcgctg gcgcggtgat gatgtcgtgc tacttgacac cgaaaattcc gattgttgcc
     4981 gatgagcaga tcgagaggat ggttagcgac gaagccggtc ggatcctggc cgcggccgat
     5041 ccacgcgccg agttttccca ttaccgtttt cagctttcag cgtttccacg gcaggatctg
     5101 ctcggtgtaa gtattggccg cgggcagatc tatatcagtt atcagctcgc ccaactcgcg
     5161 ctcaaaaaat cttactatcg ctggttgctg cgccagaccc tggcccatga aattgcccac
     5221 gagcttgccg gacacgcgaa ccagcgcggc gccgttgcta acagcccggc atctcagggc
     5281 gtcacggcgc gccatctcgg gctcggtggg ctggtgcaat ttcaaaacta ttccgtcgaa
     5341 aaggagttgc aagcggattt ttacgccatg aattattggt ccgcacttgg ctgggattgc
     5401 gccatttggg tcgatatcct gcgcgggttc caaaagcaaa attacgccgg agatgttttt
     5461 cacccgaccg atcgccgcct ggaa
//
LOCUS       DASQKS010000246         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1382920, whole genome shotgun sequence.
ACCESSION   DASQKS010000246 DASQKS010000000
VERSION     DASQKS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382920"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VE170_06365"
     CDS             complement(<1..869)
                     /locus_tag="VE170_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HYJ15095.1"
                     /translation="MGKTRVHLLAKELGIETKDLIAQLDKLGMRGRKAQSAMEDEEVT
                     RVRAALVAQEKPQVHIGEEKVIARVVKTEEEGHASVESRETVVERRVRTNVIRRRTSR
                     VEVAPPEGAEEAAVAAPVEPEIITEPVVSAEPAAPPPPAAEEMPVEAAAETAPITEEP
                     ASVEPVVEAASPEVIPEQIEVKPEPVAPPPVAPPRPATEPETMEALQRTAPRGARILG
                     RIDLKQSVRVEPAPTPVLRRPAPGAAAAGATGTAAVEPGAGDDKPKAGRHKKRVVKKQ
                     DVIESREKEVRGGR"
     gene            complement(885..1154)
                     /locus_tag="VE170_06370"
     CDS             complement(885..1154)
                     /locus_tag="VE170_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HYJ15096.1"
                     /translation="MAKREDAPERTCLGCGARDRKELLLRLIVNGAGQLEVAAHGQRG
                     GYLHRRLKCWSGFVGRKSHFRAFRVEVAKPAKEKLLRELKDSVGV"
     gene            complement(1161..2540)
                     /gene="nusA"
                     /locus_tag="VE170_06375"
     CDS             complement(1161..2540)
                     /gene="nusA"
                     /locus_tag="VE170_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYJ15097.1"
                     /translation="MQQDLNRVIEQVSKEKGIDKAILISALENAMISAAKKTFGHQRK
                     IEAQFNAEIGEVELFEAKTVVETVQDEATEITLAAAHENLDPDAEVGDELLTKLDSSL
                     FGRIAAQAAKQNIVQRVRDAEREIIYNEFKGREGQLVNGIVQRFEKRNIIVNLGRTDA
                     ILPEKEQVPRERYRQGDRIRAYIVSVEITSRGPQIVLSRTHPGMLIQLFTQEVPEIYE
                     GIVEVKGAAREPGGRAKIAVVSHDHDVDPVGACVGMKGTRVQSVVQELRGEKIDIVHW
                     IPDQAEYVCRALAPAKVSKIIIDDQDHTMEVIVPDDQLSLAIGKKGQNVRLASRLTGW
                     RIDVCNEAEAEEETRRARQSIGAIPGVGDIAAELIYQEGFKSAEDVADSELAELLDIE
                     GISKEKAEAVHKSAVLYVAEKRAREAEERAKEAELAANPPPAPEPEAALAGQADVAPE
                     VPAGPEEPA"
     gene            complement(2542..3012)
                     /gene="rimP"
                     /locus_tag="VE170_06380"
     CDS             complement(2542..3012)
                     /gene="rimP"
                     /locus_tag="VE170_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYJ15098.1"
                     /translation="MDATLTRIWQMTEPLAASEGMEVVDIELKHEGSRGGRILRVYLD
                     KQGGPAMEDLSKVSRSLSDLLDENEDVVEGSYSLEVSSPGVNRPLKRPEHFAGFVGKR
                     IRVRTREAIDGRRTFLGNLLEVTPQTIAINQDGRRWEIPFSIIEKSNYEHDWSA"
     gene            3090..4295
                     /locus_tag="VE170_06385"
     CDS             3090..4295
                     /locus_tag="VE170_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ15099.1"
                     /translation="MTIAANARGREPIGRCATAVICYDFATLKPFLMKAANHSKKPAP
                     AQISWEQILSNLDDGVITADPGGKILFFNEASEVMMEISSAVATEQTLKQIFKRERWL
                     IELVDKTWPPRCESARGEGDFVTRWGGKIPVSVTASPLQDRQGTFSGTILLLRDLRHQ
                     RELEEDLKRADRLATLGTLAAGLAHEIKNPLGGIKGAAQLLRRRAESDPSVRDYTDIM
                     IREVNRVNQLIEQLLDLSRPTELNLEALNIHEILEDVLMLEGQSEAAQKYHIKKNFDP
                     SLPAIRGDRSRLTQVFLNLVKNAFQSMEKPGAVTVTTRVETDFHIRGNGNRRRRLIWV
                     DIADQGAGIRDEDLPHIFSPFFTTKMNGTGLGLATCYQIVKEHGGMIRVESSPGKGSV
                     FKISLLVAN"
     gene            4298..5743
                     /locus_tag="VE170_06390"
     CDS             4298..5743
                     /locus_tag="VE170_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ15100.1"
                     /translation="MDQGKILVTEDEESLRFVLQKALEGEGYWVQTAANGATARRLLT
                     ESRFDVSLMDIKLPDADGLILLKEFKDSGIDTAMILMTAQNTMRNAIGAMKNGAFDYI
                     TKPFDLDEVLVLVKRAMDSRKLTRDFRELKEEVKKRFEPGVNIIGTSSSMQGVYKTIG
                     QVVDNTASILILGESGTGKELVAKTIHYNSPRWNQPFIAINCAAIPRDLLESELFGHE
                     KGSFTGAMDRRLGKFELAEGGTLFLDEVGDIPLDLQTKLLRVLQDHEYSRVGGRDILK
                     ADVRILAATNQDLEKAVKEKRFRDDLYFRLKVIPIHLPPLRERRGDITLLIGYFIEKI
                     NREMGIQISGISPEAQKLLEDHSWPGNVRELENTLIRAAVLSSGPILFPKDFTLQNKP
                     AATTLEVDQLSLEEIIRHKLEDYFRRMEGVDVDNLYSLVIERIERPLIELTLRKTKGN
                     QIKAADILGINRNTLRKKISDLGIELKKDPT"
     gene            5748..>6048
                     /locus_tag="VE170_06395"
     CDS             5748..>6048
                     /locus_tag="VE170_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYJ15101.1"
                     /translation="MALTLPPLYAILDPDQIKGRAIEEVLGEMLRAGVTMLQLRVKSL
                     VAKDFLALGQRVREATETHHCRLIVNDRVDIAMACNADGVHLGQDDLPLQAGRKLL"
BASE COUNT         1223 a         1893 c         1652 g         1280 t
ORIGIN      
        1 cggccgccgc gcacttcttt ttcgcgcgac tcgatgacat cctgtttctt gacgacgcgt
       61 tttttatgac gccccgcctt gggcttgtca tcaccggcgc ccggttccac cgcggcagtg
      121 cctgtcgcac cggccgccgc cgccccagga gcgggccggc gcaataccgg cgtaggcgcc
      181 ggttcgactc ggacagactg cttgagatcg atgcggccaa gaatgcgcgc accgcgcggt
      241 gcggtacgct gcaaagcttc catggtttcc ggttcagtcg ccggtcgagg tggggcaacc
      301 ggcggcggcg ccactggctc cggtttaact tcaatctgtt ccggtatcac ttcgggtgaa
      361 gcagcttcga cgaccggttc aaccgatgcg ggttcttcag ttattggcgc tgtctccgcg
      421 gcggcttcca ccggcatttc ctccgcagca gggggcggag gggcagccgg ctcggcgctg
      481 accaccggtt ccgtgattat ttcaggctca accggcgcag cgacagctgc ttcttccgcg
      541 ccttcggggg gtgcgacctc aacccggctg gtgcgccggc gaatgacatt ggtacggacc
      601 cggcgttcga cgacggtctc acgcgactcg acgctggcat gaccttcttc ctcggtcttg
      661 accacgcgag cgatgacttt ttcttcaccg atatggactt gcggtttttc ctgggctacc
      721 aacgcagcgc gaacacgggt cacttcctca tcttccatcg cgctctgggc cttccgaccg
      781 cgcatcccca acttgtcgag ctgggcaatc aaatctttgg tctcgatgcc caattctttt
      841 gccagcagat gaactctcgt ctttcccatt ttactccccg tcgtttatac cccgacactg
      901 tccttcagtt cgcgcagcaa tttttccttg gccggcttgg cgacttcaac acgaaatgcc
      961 cggaagtggc tctttcggcc gacaaagccg ctccaacatt ttagccgtcg atgtaaataa
     1021 ccaccgcgct gcccgtgcgc cgcgacttcc agttggccag cgccgttgac gattaagcgc
     1081 agcaggagct ctttacgatc acgcgcgccg cagcctaaac aggttcgctc cggcgcatct
     1141 tcccgtttcg ccaccggatg ctaagccggt tcttccggcc ccgctgggac ttccggcgcc
     1201 acatcggctt ggcccgccaa agctgcttca ggttcaggcg caggcggcgg gttggcggcc
     1261 aactccgcct ccttggcgcg ctcctccgcc tccctggcgc gtttctccgc tacatataag
     1321 accgccgact tgtgcactgc ttcagccttt tctttgctaa tgccttcgat atcgagcaac
     1381 tcagccagct cgctgtcagc tacgtcttcg gccgatttga atccttcttg gtagatcagc
     1441 tcggcagcga tatcgccgac acccggaatc gctccaatcg actgccgcgc ccgccgcgtc
     1501 tcttcttccg cttccgcttc attgcacaca tcgatgcgcc agcccgtcaa acgcgacgct
     1561 aagcgcacat tctggccctt tttaccgatc gccagcgaca gctggtcgtc cggtacgatc
     1621 acttccatgg tgtgatcttg gtcgtcgatg atgatcttgg agactttggc tggcgccagg
     1681 gcgcggcaaa catattccgc ctgatcggga atccagtgca cgatatcgat tttctcgccg
     1741 cgcagttctt gcaccaccga ctgaactcgc gtgcccttca tgccgacgca agcgcccacc
     1801 ggatcgacat catggtcgtg ggaaactacg gcgatcttgg cgcggccgcc aggttcccgg
     1861 gctgcgcctt tgacttcgac gatgccttcg tatatctccg gcacctcctg cgtaaacagt
     1921 tggatcaaca tgcctggatg ggtgcgcgat aaaacaatct gcgggccgcg gctggtgatc
     1981 tcgacgctga cgatataggc gcgaatccga tcgccttggc ggtaacgttc gcgcggcacc
     2041 tgttcttttt ccggcaggat cgcgtcggtg cggccgagat tgacgatgat gttacgcttc
     2101 tcgaagcgct gcacgatacc gttgaccagc tggccttcgc ggcctttgaa ttcgttgtaa
     2161 atgatttctc gctcggcgtc gcgcacgcgc tgcacgatat tttgcttggc cgcctgagcg
     2221 gcgatgcggc cgaacagact cgaatccaac ttggtcagca gttcgtcgcc cacctccgcg
     2281 tctgggtcga ggttttcatg ggccgccgcc aaagtgattt cagtggcttc gtcttgtacg
     2341 gtctcgacca ccgtcttggc ttcgaagagc tcgacttcgc cgatctcggc attgaactgt
     2401 gcctcgatct tgcgctgatg gccgaaggtt ttcttggccg ccgagatcat ggcattctcc
     2461 aaagcgctga tgaggatcgc tttgtcgatg cctttttcct tgctgacctg ctcgataact
     2521 cggtttagat cctgctgcat attaggcact ccagtcgtgc tcgtagttgg acttttcgat
     2581 aattgaaaat ggaatttccc agcgcctgcc gtcttgattt attgcgatgg tctgcggcgt
     2641 gacttcgagc aggttgccaa gaaaggtgcg ccgcccgtcg atcgcctcgc gcgtccgcac
     2701 ccggattcgc ttaccgacga aaccggcaaa atgctcgggg cgcttgagcg gccggttaac
     2761 cccgggcgaa gaaacttcca gggaataaga accttcgacg acgtcctcgt tctcgtccag
     2821 caaatcgctc aagctgcggc tgactttgct caagtcttcc atcgccgggc cgccctgttt
     2881 atcgagatag acgcgcagta tcctgccgcc ccggctgcct tcatgtttga gttcgatatc
     2941 gacgacttcc atcccctcgc tggccgccag cggttcggtc atttgccaga tccgggtgag
     3001 ggttgcatcc atgcctgctt gccccagaaa caaaaaaagc gggcagagcc cactttgagt
     3061 actactccta agtgtagaga atttctacca tgacgatcgc cgccaatgca agggggcgag
     3121 agccgattgg ccgttgcgcc accgcggtca tctgctatga tttcgccacg ttaaagccat
     3181 ttctcatgaa agccgcgaat cattccaaaa agcccgcgcc ggcgcagatc tcgtgggagc
     3241 aaatcctcag caacctcgac gatggcgtga tcaccgccga ccctgggggc aagattctgt
     3301 tcttcaatga agcctcggaa gtcatgatgg aaatttcctc ggccgtcgcc accgaacaga
     3361 ccctaaagca aattttcaaa cgggaacgct ggttgatcga actggtcgac aagacctggc
     3421 cgccgcgttg cgagagcgcc cgcggcgagg gcgactttgt cacccgttgg gggggcaaga
     3481 tacccgtcag cgtcactgcc tcgccgttgc aggatcgtca gggcacgttc tccggaacca
     3541 tcctgttgtt gcgcgatctg cgccaccagc gcgaacttga agaggacttg aaacgtgccg
     3601 atcgcctggc gactctggga accctcgcgg cgggcctcgc ccatgaaatc aaaaacccct
     3661 tgggcggcat caaaggcgcg gcgcagttgc tgcggcggcg cgccgaaagc gatccgtcgg
     3721 tgcgtgacta caccgatatc atgatccgcg aagtcaaccg ggtgaaccaa ttaattgagc
     3781 agctgctcga cctatcgcgg ccgaccgaac tcaatttgga agcgctcaac atccacgaga
     3841 tcctggaaga cgtgctcatg ctcgaaggtc aatcagaagc ggcgcaaaaa tatcacatca
     3901 agaaaaattt cgatccgagc ttgccggcga ttcgcggcga ccggagccgt ctgacccaag
     3961 tattcttgaa tctggtcaaa aacgcttttc aatccatgga aaagccaggc gcggtgaccg
     4021 tgacaacgcg agtggaaacc gattttcata tccgcggcaa tggcaatcgc cgcaggcggc
     4081 tgatttgggt ggatatcgcc gaccaagggg ccggcattcg cgacgaagat ctgccgcata
     4141 tcttttcgcc ctttttcacg actaagatga atggtacggg cttaggcctg gcgacctgtt
     4201 accagatcgt caaggagcat ggcggcatga ttcgcgtcga gagcagtccg ggcaagggca
     4261 gcgtgttcaa aatttcacta ctggtagcaa actagcaatg gatcaaggca aaattttagt
     4321 caccgaggac gaagaatcgt tgcgcttcgt gctgcaaaaa gcacttgaag gcgaaggcta
     4381 ctgggtgcag accgccgcca acggcgccac cgcccgccgg ctgctgaccg agagccgttt
     4441 cgacgtctcg ctcatggata tcaagcttcc cgacgccgac gggttaattt tgttgaagga
     4501 attcaaagac agcggcatcg acaccgcgat gatcttgatg accgcgcaaa acaccatgcg
     4561 caacgccatc ggcgcgatga aaaatggcgc cttcgactac atcaccaaac ccttcgatct
     4621 tgacgaagtg ctggtcttgg tcaagcgcgc catggacagc cgtaagttaa cccgcgactt
     4681 ccgcgaattg aaagaagaag tcaagaaacg cttcgagccc ggcgtcaata tcatcggcac
     4741 cagctcgtcc atgcagggcg tctataaaac catcggccag gtggtcgaca acactgcgtc
     4801 catcctgatc ctaggcgaga gcggcaccgg caaggaattg gtcgccaaga ccattcatta
     4861 caactcgccg cgctggaacc aaccgttcat cgccatcaac tgtgccgcca taccgcggga
     4921 tcttctagag agcgagctgt tcggccacga gaaaggctcc ttcaccgggg ccatggaccg
     4981 ccgcctcggc aaattcgagc tcgccgaagg cggcacgctg ttcttggacg aagtcggcga
     5041 tatccccctc gacctccaga ccaaactgct gcgcgtgctc caggatcacg agtatagccg
     5101 cgtcggcggc cgggacattc tcaaagccga tgtgcgcatc ctcgccgcca ccaatcagga
     5161 tttggaaaaa gccgtcaaag aaaaacgctt ccgcgacgat ctctacttcc gcctgaaagt
     5221 catccccatc cacctgccgc cgctgcgcga gcggcgcggc gacatcacgc tcttgatcgg
     5281 ctacttcatc gaaaaaataa accgcgagat gggcatccag atttccggca tttcgcccga
     5341 ggcgcaaaag ctgctcgaag accactcctg gccgggcaac gtccgcgagt tggaaaacac
     5401 cctgattcgc gccgccgtgc tgtcctccgg acccatcctc ttccccaagg atttcacgct
     5461 gcagaacaag ccggcggcga cgacattgga agtcgaccaa ctgtccctcg aagaaatcat
     5521 ccgccataaa ctggaagact actttaggcg catggaaggc gtcgatgtcg acaatcttta
     5581 ttcgttggtg atcgaacgaa tcgaacggcc gctgatcgag ctcacgctaa gaaaaaccaa
     5641 gggcaatcag atcaaggccg cggatatcct cggcatcaac cgcaacacgc tgcgcaaaaa
     5701 gatctccgat ctgggcatcg agctcaagaa agatccgacc tagttcaatg gcgctcaccc
     5761 ttcccccgct ctacgccatc ctcgatccgg atcagatcaa agggcgcgcc atcgaagaag
     5821 tactgggcga gatgctgcgc gccggcgtca ctatgctgca gctgcgcgtc aagtcgctgg
     5881 ttgcgaaaga tttcttagca ctcggccagc gcgttcgcga agctaccgaa acgcaccact
     5941 gccggttgat cgtcaacgat cgcgtcgaca tcgccatggc ctgcaacgcc gatggcgtcc
     6001 acctcggcca agatgacttg ccgctccagg ccggacgcaa gttgctgg
//
LOCUS       DASQKS010000247         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3907121, whole genome shotgun sequence.
ACCESSION   DASQKS010000247 DASQKS010000000
VERSION     DASQKS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907121"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VE170_06400"
     CDS             <1..937
                     /locus_tag="VE170_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15102.1"
                     /translation="GKTIRIIVGYSPGGGYDALARMLSRQMPKYIPGHPTILVENMAG
                     AGSLLAANHIFKVAKPDGLTMGHFSGGFVYSQVMGQPGIELDMQKFVFVGAVARDESA
                     IAVTKASGVNNMERWFAAKTPLKMGTTGPGAFGTDNVLKVIKVALNLPMQIISGYKGT
                     ADMRLAAESGELDGTTWGWDSMRGTWQKSLESGVVSVVLQTVPKAFADLPKVPLAIDL
                     AKTAEAKQLIEYGIHYPSKITKTLALPPGTPADRALILQKALQDTVKDPEFIAEADKA
                     KIGLAPVTVDDMKKTVDGVFKLDPALLAKLKTILF"
     gene            971..2026
                     /locus_tag="VE170_06405"
     CDS             971..2026
                     /locus_tag="VE170_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15103.1"
                     /translation="MAQLRRCLGRFIAALTILWSVPEVSAQHDEKAAADFYRGKQIRV
                     VIGSGPGGTYDIYSRLLSKHMGRFIPGHPAMLVQSRAGAGGLIAANTIYNTEPRDGTV
                     IGSFGETFPLRQAMGAPSIQFDSAKYQWIGSAINTAIACVARNDSAVASFQDIVDGKP
                     FTVGTMAPGSTIYDTPAIINAAYNLQMKLVRGFEGVANIVNAAEAKEVDGYCASWLAM
                     LTTGRHLQLFDAGVLKPILIMGDRVPDHPRLKQVPAAEALAKNEEAKQLLRAMHAPSQ
                     ITNPYVVHPNVRGERVAALRRAFTATFTDGEFLADAKKSRIEFSPSSGEQVTQVVRSI
                     MNTPPAVLAKMKKILVQ"
BASE COUNT          524 a          658 c          681 g          445 t
ORIGIN      
        1 aggcaagacc attcgtatca tcgtcggcta ttcgcctggc ggcggttacg atgccttggc
       61 gcgcatgctg tcgcggcaga tgcccaaata tattccaggc catccgacta ttctggtcga
      121 aaatatggcc ggcgccggta gcttgcttgc cgccaatcat attttcaaag tcgccaaacc
      181 cgacgggctg accatgggcc atttcagtgg tggcttcgtt tacagtcaag tcatggggca
      241 gccaggaatc gaactcgaca tgcagaaatt tgtcttcgtc ggcgcggtgg cacgggatga
      301 aagcgccatc gccgtgacca aggcgagcgg cgtcaataac atggagcgct ggtttgccgc
      361 taagacgccg ttaaaaatgg gcaccaccgg gccgggcgct ttcggcaccg acaatgtgct
      421 gaaagtcatc aaggtggcac tcaatctacc gatgcaaatc atctcgggtt acaaaggcac
      481 cgccgacatg cgcctggccg cggagagcgg tgagcttgac ggcacgactt ggggttggga
      541 ttcgatgcgc ggcacttggc agaagtccct cgaatcaggc gtggtgtccg tggtgctgca
      601 aaccgtgccg aaggcgtttg ccgatctgcc caaagtgccg ctggcgatcg atctggccaa
      661 gacggcggaa gccaaacaat tgatcgagta cggcattcac tatccgagca agatcaccaa
      721 gactctcgcc ctgccgccgg gcaccccagc cgatcgcgcg ctgatcctgc agaaggcttt
      781 gcaggatacg gtgaaggatc ccgagttcat cgccgaggcc gacaaagcca agatcggact
      841 cgctccggta accgtcgacg atatgaaaaa gaccgtcgac ggcgttttca agctcgaccc
      901 agctctgctc gccaagctga agaccattct cttctaagaa atcgcgcgga gcttcgaaag
      961 gatcttgcac atggcgcagc tcaggcgttg cttgggtcgg ttcatcgcag cgttgacaat
     1021 cctttggagc gttcccgagg tatcggcgca gcacgatgaa aaagccgcgg ccgatttcta
     1081 tcgcggcaaa cagattcgcg tcgttatcgg atcggggccc gggggcacct atgacattta
     1141 ctcgcgtctc ctgtccaaac acatggggcg cttcatccct ggccatccgg cgatgctggt
     1201 gcaatcgcgc gccggcgccg gcggcttgat cgccgccaac acgatttaca atacggaacc
     1261 cagggatggc acggtgatcg gttctttcgg ggaaactttt ccattgcgcc aggcgatggg
     1321 cgcgccgtca attcaattcg actcggccaa ataccagtgg atcggcagcg ctatcaatac
     1381 cgccatcgcc tgcgtcgcgc gcaacgattc cgcggtggcg tcgtttcagg atatcgttga
     1441 cggcaaaccg ttcaccgtcg gcaccatggc gccgggcagc acgatctacg acacgccggc
     1501 gatcatcaat gcggcttaca atctgcaaat gaagctggtg cgcggtttcg aaggggtggc
     1561 gaacatcgtc aacgccgccg aagcgaagga agtcgacggt tactgcgcca gttggctggc
     1621 gatgctcacc accgggcgcc acctacaact gttcgacgcc ggcgtgttga agccgattct
     1681 gatcatgggc gaccgcgtgc cggaccatcc ccgcttaaag caagtgcccg ccgctgaagc
     1741 gttggcgaaa aacgaggaag cgaaacagtt gctgcgcgcc atgcatgcac cgtcgcagat
     1801 taccaatccc tatgtggttc atccgaatgt gcgaggggag cgagtggcgg cgctgcgccg
     1861 agcgtttacc gcgaccttca ccgacggcga gtttcttgcc gacgcaaaga agagccgcat
     1921 cgagttctcc cccagcagcg gcgaacaagt tacccaggtg gtgcggtcga tcatgaacac
     1981 gccgccggcg gtgctggcaa aaatgaaaaa gattttggtg caatgaataa aagaaatgca
     2041 atcgacaaaa cgtgcaagaa tcccacggca agaaatcggc ttccttggat taaccgcaca
     2101 gagcgcaaag ttcgaagcgg agcaaggcaa cacggagtga tcttagcggc gagtaatccg
     2161 attcccgaca gcagatggga acattatgaa atttggcatt cacatcctcc cgacttacat
     2221 gccggatgtc gagggggcgc tgccggactt ttaccagcag atgtacgagc agattatcga
     2281 ggtggagaag cttggcttcg atcaagct
//
LOCUS       DASQKS010000248         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3071495, whole genome shotgun sequence.
ACCESSION   DASQKS010000248 DASQKS010000000
VERSION     DASQKS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071495"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VE170_06410"
     CDS             complement(<1..1866)
                     /locus_tag="VE170_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ15104.1"
                     /translation="MVKKIPTQLETHGHVRVDNYYWLRERDNPEVIRYLNDENALAAD
                     AMAHTQEFEKKLFEEIKGRIKQTDLSVPYKREDYFYYTRFEAGKEYPIYCRKLAALDA
                     PEQVMLDGNALAQGHEFFSIGGTAVSSAQDLLSYATDTQGRRIHTTYIKHLATGALLP
                     DVIANVTENLAWGNDNRTLFYGKQEETTLRQYQIWRHIVGTDAATDQLVYQEDDETFS
                     VYIFKTKSKRYLLLVSAQTITYECRYLDADEPLGDFKIFLPRARGHEYQIDHIADRFL
                     IRTNDQAKNFRLMATPVAQPGREYWREIIPHRDDVYLGDFELFKDYMVLEERARGLTQ
                     IRVVPWSGSGAHYLEFAEPAYRAHLGANMEFDTKIVRFDYTSMKTPQSIYDYDVDTRE
                     RKLLKQEEVLGGFNADDYVTERLHARAADGTEIPLSLLYRKGLRKDGRNPTLLYGYGS
                     YGVSIDAAFASPRLSLVDRGFVYAIAHIRGGQELGRQWYDDGKLLKKKNTFTDFVACA
                     EFLISERFTNPEKLFAMGRSAGGLLIGVATNLRPDLFKGVVAEVPFVDVVTTMLDASI
                     PLTAGEYDEWGDPNKKEFYDYMLSYSPYDNVARKPYPAMLITGGLHDSQVQYWEPA"
     gene            2220..3287
                     /locus_tag="VE170_06415"
     CDS             2220..3287
                     /locus_tag="VE170_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15105.1"
                     /translation="MAIIDADAHVIETEQTWNFMLEEDRRFTPEVLISKKNGIHFWRV
                     DDRVIANSNLGLNVPEDARDLTDVSARLAHMDALKIDIQVLYPTLFLRPVTARAEVEL
                     ALYRGYNRWLAHIWQLGNNRLRWIVLPPLRSMDKAIEEINFAKARGACGVFMRGHEGE
                     WLLSDPALFPIYQEAERLDLPICVHAGTGSFNYYDQYGQDVFSRFKLPSVGAFNNLIY
                     QGVPARFPKLRWSFVESTSQWLPYAVNDLVIRTQAEREFQRKYAGHALTQARPKERHI
                     EAATVLRDNRVYVACQTTDDLAYVVDCVGEDHITVGTDYGHADYSNDIEAIQTLADGG
                     KIGAAFAQKILDDNPRRLYGF"
     gene            complement(3389..4369)
                     /locus_tag="VE170_06420"
     CDS             complement(3389..4369)
                     /locus_tag="VE170_06420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15106.1"
                     /translation="MNIDLHSHFFPVEALQSPGKFENRAPKLVLENGKLSVTSQIGFR
                     PGLGAGAYDPLARIKALDEMHIDMQAISPSPILLFYWEDAAVAAHFSRRQNEAIQDVV
                     KKHPDRFIGFGSVPLQSISESIAIAREAKSMGLKGLEIGNVVADTPLDDPSFEPFFQI
                     AQELELLLFVHPLEGGLDADDPLSPILGNVLQFTFRTTLMVERMILKGMFEKYPNLRL
                     CLSHGGGLLAFNIWRLDHSYGLRADLQKRLPKKPSAYLPQMYFDTIVHSVAALDYLVR
                     VVGADRVVIGTDYPMAMGDFAAVNKVMELQIGDAERAAILGGNAARELRL"
     gene            complement(4406..4636)
                     /locus_tag="VE170_06425"
     CDS             complement(4406..4636)
                     /locus_tag="VE170_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15107.1"
                     /translation="MRSKAAQIVACMIILLAADAQVRTIFFPVTIRRRNYHLQGYRYA
                     ANHRVHAPPFGQATISAAADFLPDTLKTLNVL"
     gene            complement(5279..5449)
                     /locus_tag="VE170_06430"
     CDS             complement(5279..5449)
                     /locus_tag="VE170_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15108.1"
                     /translation="MKFENDKGDQVEINFQNFEKILPGDKPGYTPVKFKNGGEEWVRA
                     THSEILEATVEE"
     gene            5593..5814
                     /locus_tag="VE170_06435"
     CDS             5593..5814
                     /locus_tag="VE170_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15109.1"
                     /translation="MEEIYKGHRITVSAWQISDTKQWQPKVQIIWREGTDDAIKYPVI
                     TRYFATQSEAEIDGLAFAKSWIDDGKQDL"
     gene            complement(5902..6873)
                     /locus_tag="VE170_06440"
     CDS             complement(5902..6873)
                     /locus_tag="VE170_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15110.1"
                     /translation="MKLILLAFVLILQPPSPALGQERIKFPVGVSSKVLGYGTLWAAW
                     RRGYFEREGLDVDVVLMRGTAPAVQALIGNSISAALLANDGPITAVEQGADLVMVASG
                     SKITHMLMGAKNYKSFEDLRGATIGSSTLTSGTTFVLRRALKLKGLEYPRDYKLLSVG
                     GTTEAFLAMNAGQTAASMMAVPYSFRAQEMGFNLIGRMVDVFPNYLLSAFSVRRSWAE
                     TNRPVVVRFLKAVVRAKKWFEQDRKAAIEYLAKEFQLTPALAEKGLDYYLQAPAWHPE
                     LEIEMDGLKTVVDIYAEQNNLKGPIPNPEKYVDHSYLRQALKEIGLR"
     gene            7141..>7820
                     /locus_tag="VE170_06445"
     CDS             7141..>7820
                     /locus_tag="VE170_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15111.1"
                     /translation="MTILRQFWYPVLWSKDLTDKPTSVKLLDQPLVLWRVNGKVAAFY
                     DLCLHRGAALSLGWIDGDKLVCPYHGWNYGADGACVKIPSLPPDREIPAKARAKSYHV
                     QERHSLIWVCLDEPRQDIPAFPKEIDDPAFVWAPYNSDGQWKANAARMIENLADFSHF
                     PWVHAGILGDPEQAESPVIELQEVSGGFQYEIDTPVNRLRAQSPSKQRYTVILPFMVV
                     IQRSQPGG"
BASE COUNT         1788 a         2230 c         2094 g         1708 t
ORIGIN      
        1 ggccggctcc cagtactgca cttgggagtc gtgcaagccg ccggtgatca acatggccgg
       61 atagggtttt ctggcgacgt tatcgtaggg cgagtaggac aacatgtagt cgtagaactc
      121 tttcttattg ggatcgcccc actcgtcgta ttcacccgcg gtcaatggaa tcgatgcatc
      181 gagcatggtg gtcacaacgt cgacgaaggg cacctcggcg acgacacctt tgaataagtc
      241 agggcgtaag ttggtggcca cgccgatcaa caaaccgccg gcgctgcgtc ccatggcgaa
      301 cagtttttcc ggattggtga agcgttcgct aatcaaaaac tctgcgcaag cgacgaaatc
      361 ggtaaaggtg tttttcttct tcaacagctt gccatcatcg taccattggc ggcccaactc
      421 ctggccgccg cgaatatggg cgatggcata gacaaaaccg cgatcgacca gactcaagcg
      481 cggcgacgcg aaggcggcat cgatgctcac gccgtaggag ccgtagccat acagcagtgt
      541 tggattccgc ccgtctttcc taagcccttt gcgatagagc agggacagcg gaatctcggt
      601 gccgtcggcg gcccgcgcat ggagccgctc ggtgacgtag tcgtcggcgt tgaaaccgcc
      661 gagcacttcc tcttgcttta ataatttgcg ctcgcgcgtg tccacgtcgt agtcataaat
      721 cgactgcggt gtcttcatcg aggtgtaatc gaaccggacg attttggtgt cgaactccat
      781 attggcgccg agatgcgccc ggtaggccgg ctcggcaaat tctagataat gggcgccgct
      841 acccgaccac ggcacgacgc ggatctgtgt caggccgcgc gcccgctcct ccagaaccat
      901 atagtctttg aatagctcga aatcaccaag gtagacatcg tcgcggtggg gaatgatttc
      961 gcgccaatac tcgcgccccg gctgcgccac cggcgtcgcc atcaagcgaa agtttttcgc
     1021 ctgatcattg gtgcggatga ggaagcggtc agcgatgtga tcgatctgat actcatggcc
     1081 gcgcgctcgc ggcagaaaaa ttttaaaatc accgagcggt tcatcggcgt cgaggtaccg
     1141 acactcatag gtaatcgttt gcgccgacac cagcagtaag tagcgcttgg atttagtctt
     1201 aaaaatgtac acgctgaaag tctcgtcgtc ttcttggtag accaactggt cggtcgccgc
     1261 atcggtgcca acgatatgac gccagatctg atactggcgc agcgtggttt cctcctgttt
     1321 gccgtaaaac agcgtgcggt tgtcgttgcc ccaggcgaga ttctccgtaa cgttggcgat
     1381 cacatccggc agtagcgcgc cggtggccaa atgcttgata taggtcgtgt ggatccgccg
     1441 tccctgagta tccgttgcat aactgagcag gtcctgcgcc gaactcaccg cagtgccgcc
     1501 gatggagaaa aactcatggc cctgggccaa ggcattgccg tcgagcatca cttgctcggg
     1561 cgcatcgagc gcagccagct tgcggcagta gattggatac tctttccccg cctcgaagcg
     1621 ggtgtaataa aaatagtctt cgcgtttata cggcacggac aaatcggttt gcttgatccg
     1681 acctttgatt tcttcaaaaa gctttttctc aaattcctga gtatgcgcca tcgcgtcggc
     1741 ggcaagggcg ttctcatcgt tcaagtagcg aatgacttcg ggattgtctc gttcgcgcag
     1801 ccaatagtaa ttgtccacgc gcacatggcc atgggtttcg agctgggtgg ggatcttctt
     1861 gaccatcgat tcaaagcctc cgtcgcactt ttccaatgaa agtagaatct taggaaattt
     1921 ccctcggtag cgctagtcgc tttggaatag ggatattgct gtcttccatc aggtcggggg
     1981 aatccaaagt taacctttgc gaaccaatac ggagaccgct ctggtaattg agctcaaata
     2041 agcgaatgtc cagcatcgcc gccgaaccaa gcagattgct cgggaagcga attatccggc
     2101 gttgctttcg tttgacagta gcgggtgggc agttctacaa gaaatcagcc attccatctg
     2161 ggcgagccca gaaaaacgtt ctagcgcgag ccacgatctt aaaaggagaa tccatcgcca
     2221 tggcaatcat cgacgccgat gcccacgtta tcgaaaccga acagacgtgg aacttcatgt
     2281 tggaagaaga tcgccgcttc actcccgagg tattgatctc gaaaaagaac ggtatccatt
     2341 tctggcgggt cgacgaccgc gtcattgcca attccaatct cggcttgaac gtgccggaag
     2401 acgcccgtga cctgaccgat gtctcggctc gcctggcgca tatggatgcc ttgaagatcg
     2461 acatccaagt gctctacccg accctgttcc tgcggccggt gacggcgcgt gccgaagtgg
     2521 agctggcgct ctatcgcggt tacaatcgct ggctcgcgca tatctggcag ctcggcaaca
     2581 atcgcctgcg ctggatcgtc ctgccgccgc tgcgatccat ggacaaagcc atcgaggaaa
     2641 tcaacttcgc caaagcccgc ggcgcttgcg gcgtcttcat gcgcggtcac gagggcgaat
     2701 ggctgctctc cgatcccgcg ctgttcccca tctatcaaga agccgaacgg ctcgacctgc
     2761 cgatctgcgt ccacgccggc accggcagtt ttaactacta cgatcaatat ggccaggatg
     2821 ttttctcgcg cttcaagctg ccgtcggtgg gcgcctttaa taatttgatc tatcaaggcg
     2881 tgccggcgag gtttcccaaa ttgcgctggt cgtttgtcga aagcacgtcg cagtggctgc
     2941 cctacgcggt caacgatctc gtcatccgca cccaggccga gcgcgaattc cagcgcaagt
     3001 acgccggcca tgcgctcacc caggcgcggc ccaaggagcg tcacatcgaa gccgccacgg
     3061 tgctgcgcga taaccgcgtc tacgtcgcct gccagaccac cgacgacctc gcctacgtgg
     3121 tcgactgcgt cggcgaagat cacatcaccg tgggcaccga ctacggccac gccgactatt
     3181 ccaacgacat cgaagcgatt caaaccttgg cggatggcgg caagattggc gcggcgttcg
     3241 cgcaaaaaat cttggacgat aatcccagac gactatacgg gttctaattc acggccgtat
     3301 cactcaacgc agaaatgcag acacggagaa atacgttttt ttaacttgcg taaatcggcg
     3361 ctcggcgttg aatcatcttt cgaccggctc acagccgcaa ttcgcgcgct gcgttaccgc
     3421 cgaggatcgc ggcgcgctcg gcgtcaccga tctgcagctc catgacttta ttcaccgcgg
     3481 caaaatcccc cattgccatc gggtagtcgg tgccgatcac cacccggtcg gcgcccacca
     3541 cccgcacaag ataatccagc gccgccaccg aatggacgat ggtgtcgaaa tacatttgcg
     3601 gcaaatatgc cgacggcttt ttcggcaacc gcttctgtaa atccgcccgc aagccgtagg
     3661 aatgatccaa acgccagatg ttgaaagcca acaagccgcc gccgtgggac agacataatc
     3721 gcaggttggg atatttctcg aacatgccct tgagaatcat gcgctcgacc atcaaggtcg
     3781 tgcgaaaggt gaactggaga acgttgccga gtatcggcga cagcggatcg tcggcgtcga
     3841 gtccgccttc gagaggatgg acgaatagca acagctccaa ctcctgggcg atttgaaaga
     3901 acggctcgaa gctcggatcg tcgagcggcg tgtccgccac cacgttgccg atctccaacc
     3961 ccttgaggcc catgcttttc gcttccctgg cgatggcaat ggattcggaa atgctctgca
     4021 gcggcacact gccgaagccg ataaaacgat ccggatgttt tttgacaaca tcctgtatgg
     4081 cttcattctg ccggcgcgag aaatgcgccg ccaccgcggc atcttcccag taaaaaagca
     4141 aaatcggcga tggcgaaatc gcctgcatgt cgatgtgcat ttcatcgagc gccttgatcc
     4201 gcgccagcgg atcgtacgcg cccgcgccca gcccagggcg aaaaccgatc tgcgatgtca
     4261 ccgacaattt gccgttctcc agcaccaact tcggcgcgcg gttttcgaac ttgccgggac
     4321 tctgcagcgc ctcgacggga aagaaatgcg aatggaggtc gatgttcatg ctgttgcctt
     4381 ccggcgcaaa gattcggatt gacgcttata acacattcag cgttttcaaa gtatctggca
     4441 gaaaatcggc agctgcgctg atcgtggctt gtccgaatgg cggcgcgtga actcgatgat
     4501 tcgccgcata tcgataacct tgcaggtgat aattccgccg ccggatcgtc acaggaaaaa
     4561 atatcgtccg gacctgagca tccgccgcga ggagaatgat catacatgca acgatttgcg
     4621 cggctttact tctcaccact agcctctgct ttgtcctgtt cacaaataat cacccatgca
     4681 gtgatttgcg ttgctctact ttttcatttg gttcgtctgc ttctcgtagt gacagatccg
     4741 gcgaacgcag tggctgggca ctaatcctgc ttccactctt ccactataga ggtagcagaa
     4801 gacgacaggg tgtctcagat cagagacacc ccgttggcga aagcgccctg gaaaggagaa
     4861 gcgttatgcc ttggtgctat gtatgcgtca ataattttcc gcgacagaat cacgaagaga
     4921 gcgagcagca ccaactggcg accgcgcgcc tcgtaataag cgatgccgac gccggctact
     4981 gcgctttttg cggcttggca accggtagag tgacagagct caaatcgtgc cttgatctgg
     5041 accacgcgaa aaaattggcc gaggcgcgta aattattgga ggccaaataa gaatagggat
     5101 agattagcag gcacgaaacg gcgttaaaga atgaccactt cgagagagtt gaaaggtgat
     5161 acgggattaa gcgattttca tgaatccaac cgagaacacc ccgcaggtcg gctgctccgt
     5221 cgagtggaaa aaaatcgtcg gcgttatcca gatgataatg tcgcaactct cccgacttct
     5281 actcttccac agtggcttcg agaatctcgc tgtgcgtcgc ccgcacccat tcttctccac
     5341 cgttcttaaa tttcacgggc gtatagccgg gtttgtctcc cggcaatatc ttctcgaaat
     5401 tctgaaagtt tatttccact tgatcgcctt tgtcgttttc aaatttcatg gcctaaaaat
     5461 accacaatag gatggcgggt ccacacctaa ccaatcaata aaaccaaaaa actcagaatc
     5521 tccgcgatac ttattcctgg tttttatcat ggtagaatac gaaaaaaccc gactgaaggt
     5581 taggctgaag taatggaaga aatctacaag ggacatagaa tcacggtgtc agcgtggcag
     5641 ataagtgaca ccaagcaatg gcagcccaag gtccaaataa tttggcgtga gggaaccgac
     5701 gacgcgatca aatatccagt gataaccaga tactttgcca cgcaatcgga agcagaaata
     5761 gacggcttag cattcgcaaa aagttggatc gatgacggca aacaagacct ataacgggcg
     5821 cacctgtgag ctagttgtct gaacgtgtca tgacgagctg atgccgatca ggatttgccc
     5881 ggcgcgcata atgtacttgg gtcatctcaa cccaatttcc ttcagggctt ggcgcaaata
     5941 actgtgatcg acatacttct ccgggttggg gatcggccct tttaaattat tctgttcggc
     6001 gtagatgtcg acgacggttt tcaagccgtc catttcaatc tccagttcag gatgccaggc
     6061 gggagcttgc aggtagtagt ccagcccttt ttccgccagc gccggagtca gctgaaattc
     6121 cttcgccaag tattcaatcg cggctttgcg atcctgctcg aaccattttt ttgcgcgcac
     6181 caccgccttg agaaagcgca caaccaccgg gcgattcgtc tcggcccaac tgcgccggac
     6241 ggaaaatgcc gagagcaaat aattgggaaa gacatcgacc atgcggccga ttaaattaaa
     6301 ccccatctcc tgggcccgaa aggaataggg cacggccatc atcgaagcgg cggtttgacc
     6361 ggcattcatg gccaggaacg cctcggtggt accgccgacg ctcaggagtt tataatcgcg
     6421 cggatactcg agtcccttga gcttgagagc gcgccgcaac acgaaagtcg tgccggaagt
     6481 taacgtggac gaaccgatgg tcgcgccgcg caggtcttcg aaacttttat aattctttgc
     6541 acccatcagc atgtgggtga ttttgctgcc gctggcgacc atcaccaaat cagcgccctg
     6601 ctcgacggcg gtgatcggcc cgtcgttggc gagcaatgcc gcggaaatcg agttgccgat
     6661 caacgcttgc accgccggcg ccgtgccgcg catgagcacg acgtcgacat cgagcccttc
     6721 acgctcgaaa tagccgcgcc gccaggccgc ccacagagtg ccataaccca atactttgga
     6781 cgaaacgccg acgggaaatt tgattcgctc ctggcccaac gccggcgacg gcggctgcaa
     6841 gatcagtaca aatgcgagca gaatcagctt catattcgat ttcctcgtgg tttcccgcca
     6901 catctatctc agtaaaactc gccgccttgt aacacggcat atcgcgattt cctagcaaaa
     6961 tgctaggccg tagcgaggtc ccgcttctac gctcgaaatg acaggttgcg tttcgtcgtc
     7021 atcccgaacg cagagaggga tctagccaaa ctgaaccgag accccatggc ggcgctcgct
     7081 tgacacaggc gtgccacaat ctgctaatac aattggtgat cgattatcag gagcatacgt
     7141 atgactattc tccggcaatt ttggtatccc gttctctggt cgaaagatct gaccgacaaa
     7201 cccacgtcgg tgaaactgct cgaccagccg ttggtgctct ggcgcgtcaa tggcaaagtc
     7261 gcggctttct atgatctctg cctgcatcgt ggcgcggcac tgtcattggg ctggattgac
     7321 ggcgacaagc ttgtctgtcc gtaccatggc tggaattacg gcgcggacgg cgcgtgcgtg
     7381 aagattccct cgctgccccc cgatcgtgaa atacctgcca aggcacgggc gaaatcttac
     7441 catgtgcagg agcgccatag tctgatctgg gtttgtctcg acgaaccccg acaagacatt
     7501 ccggcatttc ccaaggagat cgatgatcct gctttcgtct gggcgcctta taattccgac
     7561 ggccaatgga aagccaatgc cgcgcgcatg atcgagaatc tcgccgattt ctcccacttt
     7621 ccctgggtcc acgccggcat cctcggcgac cccgaacagg ccgaatcgcc ggtaattgaa
     7681 ctccaggaag tgtccggtgg ttttcaatat gagatcgata ccccggtcaa tcgtctgcgc
     7741 gcccagagcc cgtcgaagca acgctacacc gtcatcctgc cgttcatggt ggtcatccag
     7801 cgcagccagc cgggcggtga
//
LOCUS       DASQKS010000249         5919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3493056, whole genome shotgun sequence.
ACCESSION   DASQKS010000249 DASQKS010000000
VERSION     DASQKS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493056"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1164)
                     /locus_tag="VE170_06450"
     CDS             complement(214..1164)
                     /locus_tag="VE170_06450"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYJ15112.1"
                     /translation="MAVDDSKVMKPGFIVGVPRSGTTLLINLLGQHPLIAPLYETRFL
                     RNLLVLCRSASWYYGNTVGRQIARVAEPMVRASFQRACNTYERKAVRYSVVPDEGNGL
                     KQRYESFPFGESYCIHYRLDELVKETSDWLASLRMGKAQPEEVYSSARLYVDRLFAIH
                     CARMNKPNWINKTPGLLTYLDTLPKLFPHSKCIHIVRDGRDVAVSNLSLSWGPKTVRA
                     AARRWKKLIMDGRKAARASVIDYLEIRYEDVVEDSQRMLRQALRFFELEDNIVSTMVV
                     ISGRKDAWRNKLSAEDRRVFAQEAGDLLIEFGYAHDDSWV"
     gene            complement(1300..1947)
                     /locus_tag="VE170_06455"
     CDS             complement(1300..1947)
                     /locus_tag="VE170_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ15113.1"
                     /translation="MKFLEDLRRPLKILDVGTADGYLGAALKQQGHYVVGVEKDEFLA
                     KAARPYYAKFYRADLETFDFPFHAEFDFIVFADVLEHLREPEVILGRSLSCLNATGQV
                     IVSVPNIANLLIRIMLLFGKFDYQDRGILDRTHLRFYTLSSFTALLQNAACRVVDIVP
                     TPLPVQLIFPWTQRPIFALIHQIHYATVRLWKTLLAYQFVARALPCQERNGLRNS"
     gene            complement(2019..2795)
                     /locus_tag="VE170_06460"
     CDS             complement(2019..2795)
                     /locus_tag="VE170_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ15114.1"
                     /translation="MAVALNQPKVIVVMPAYNAAKTLRITYDAIPKDDVYQVILVDDG
                     SRDETLKIAKELNLEVFVHTRNFGYGGNQKTCYTEALKAGAEIVVMLHPDYQYDPSLL
                     PNVIAPIKNGDADVVLGSRLLAGEVVKQGMPWWKYLGNRFLTGIQNQVLRQSLAEYHT
                     GYRAFSRRVLEEVPFLLNSDNFVFDQEMLVQVVHLGFRIKEVPVPTRYFAEASSASFA
                     ASVVYGVSILCVLLRYVLHRSAWIAQKQFECFPARYRRAE"
     gene            complement(2821..3696)
                     /locus_tag="VE170_06465"
     CDS             complement(2821..3696)
                     /locus_tag="VE170_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HYJ15115.1"
                     /translation="MGRPPSVYPNFILHRSGRCRIWLDANHAHPAVLALLKKPDELFD
                     LPGCEVIKDQRKIKVARVALELGGARRRIYVKRYNTFSLRHRLGSIFSPSGGIRSLRG
                     AAILTRNGIATAKPIAAVEERIWGAVTWSFFLSEEIQGGVTVDSLWREILSSRPTPEG
                     PRSRRAFLTALGTLFHALHSQGVYHNDLKDANILAVPITDSERTALFLLDLEGVRQYR
                     ALSESRRIKNLVQLNRTLGRYLRNVQKLRVLQAYLNRESASRQLRRRIIERVVRESKR
                     LDAVKLRHGVGRPLL"
     gene            complement(3668..4822)
                     /locus_tag="VE170_06470"
     CDS             complement(3668..4822)
                     /locus_tag="VE170_06470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ15116.1"
                     /translation="MKIALVHKRLDLKGGTERDLYQTAEGLRDRGHQVHLFCSEYGVP
                     TPRGTIGHTIPVVRLGRTLRLWSFALAAPRIIKNQRCDVVVSFGRLLSQDILRSGGGT
                     HRGFLLRMGQQRGAWRRLRQRLSPYHQSLLRIERKQFSDGHFKKVIAVSDEVKCDIER
                     HYRVENDKIEVLYNGVDAQRFHPARRTQVREIQRARWKVPNQSPLVLFVGSGFARKGL
                     DRLIAIWNRPPLTHVYLMVAGDDGRMGHYQNWAKSVAGERILFVGRQDDIETYYAMAD
                     LVALPALQEAFGNVVLESLASGVPVVVSQEVGAGAVLTGCLRQGVVEDAGDSTQLEAR
                     LCNMLEHCAKPEFRDAARQLGEQYSWNSHFHRLEALLNEVCHGPPAIRVS"
     gene            complement(4819..5547)
                     /locus_tag="VE170_06475"
     CDS             complement(4819..5547)
                     /locus_tag="VE170_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ15117.1"
                     /translation="MPNNAIFDAVSSAWSTEDPVGKRILDLSCGGGDTAEMLARLGYK
                     VTATDYPPPPSMTPGIERVAGVDLNAFLPFRTASFDAIDLVEVIEHIENQPQLIRELA
                     RVLKPGGVALISTPNVLNLMSRVRFLFTGFLRGRVRPAHYSRKPGQAPNIYLLHFYEL
                     YYLLFHYGFEVAGLSKTRVKFAARLFMIIFWPFMWLFSFMTVIHAEKDPLQRRYNWQI
                     LSYLFHPALLLSDNIVVKAKKTHV"
     gene            complement(5642..>5919)
                     /locus_tag="VE170_06480"
     CDS             complement(5642..>5919)
                     /locus_tag="VE170_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15118.1"
                     /translation="HYSPWLTGNLRKRNLVQLNRLPLAHITLQDRLRFQHAYLGVRRL
                     TARDRRFARWLEARTRQRRNECDGVDPSGSFRKLMCLVPETTSAQNG"
BASE COUNT         1462 a         1647 c         1474 g         1336 t
ORIGIN      
        1 aatcttgatt ttcaagatcg gttccgggat ctgctgctcg acgagatttt tcgcttgatt
       61 gaggagcgtc caatcaatat ccatccgcac caactctctt tgagactgct ctgttcatct
      121 cgaacattcg cggttcgccg cagaccggcc cagacgcatt ccgtttatga tccgcgtatc
      181 acagacgcgc atgattcgtc gattcaaccg cgactatacc caactatcat catgcgcgta
      241 gccaaattca atcaaaagat cgcccgcttc ttgagcaaag acacgtcgat cttcggcgct
      301 caacttgttg cgccaggcgt ccttgcgccc actgatgacg accatcgtcg aaacaatatt
      361 atcctccaat tcaaaaaacc gcagcgcttg ccgcaacatt cgttgagaat cctcaaccac
      421 atcttcgtag cgaatctcca gataatcgat aacggatgca cgagcggctt ttctgccgtc
      481 cattatcagc tttttccacc tgcgggcagc tgctcggacc gtcttcgggc cccaggacaa
      541 cgataaattt gaaaccgcga catcccggcc gtctcgaacg atatggatgc atttactgtg
      601 cggaaaaagt ttgggcagag tgtctagata ggtcagcagc ccgggagtct tgtttatcca
      661 attgggctta ttcatgcgtg cgcaatggat tgcgaaaagc cgatcaacgt atagtcgtgc
      721 agaactataa acttcttcgg gttgtgcctt ccccatacgc aatgaagcta accaatcact
      781 ggtttccttg accaactcat ccaagcggta atgaatgcag taactttcgc cgaatggaaa
      841 tgactcgtaa cgttgcttaa ggccgtttcc ctcgtctgga acaacgctat agcgaactgc
      901 cttccgctca taggtattgc aagctctttg gaaagacgca cgcaccatcg gttcggcgac
      961 gcgggcgatc tgtcggccga cggtatttcc ataataccaa gaagccgatc gacacagaac
     1021 caacaggttc cgcaaaaatc gagtttcata gagcggcgcg atcaatggat gctgacccaa
     1081 gagattaatt aacaacgtgg ttcccgatct tggcacaccg acgatgaatc ctggtttcat
     1141 tacctttgag tcgtctaccg ccattcaaaa tattcccgta tcgacgccgg aaataacccg
     1201 gtcaagcagc gcggatctgt cgagttatcc aattttcgga acggacgctc gggagatgca
     1261 agggaatcga cccgaccgag ccagctaagt taattctcgt taagagttgc gtaagccgtt
     1321 gcgctcctgg caaggcagag cgcgagcgac aaactgataa gcaagcaagg ttttccatag
     1381 tctcaccgtc gcgtaatgta tctggtgaat tagcgcgaaa attggtcttt gcgtccacgg
     1441 aaaaatcagt tgcacaggca atggcgtcgg cacaatatcg acaacccgac aagccgcgtt
     1501 ttgtaacaac gcggtaaaac tagataacgt ataaaagcgc agatgggtgc gatccaagat
     1561 acctcgatct tggtaatcaa atttaccgaa tagcaacatg atccgaatta ataaatttgc
     1621 aatgttcgga acagaaacaa taacctgacc tgtcgcattt aggcaggaaa gtgaccgtcc
     1681 taggatcact tcaggttcgc gcaaatgttc taggacgtcg gcaaaaacaa taaaatcgaa
     1741 ttccgcatga aacgggaagt cgaaggtttc gagatcagcg cgatagaatt tagcataata
     1801 gggccgagca gcctttgcca agaattcgtc tttttcaacc ccaacaacgt aatgcccctg
     1861 ttgcttaagg gccgcaccca agtatccatc ggcggtgccc acgtccaaga tttttagcgg
     1921 ccgccgcaaa tcttccagaa acttcaatat ccagtagtga ctactatatt tgaaacattt
     1981 taatcggtaa ttaagttctt ggatttttgc cgcgtccctc actccgccct ccggtatcgg
     2041 gcgggaaaac attcgaattg tttctgcgcg atccacgcgg agcgatgcag tacatatcgt
     2101 aacagcacgc atagaatgct caccccgtag accacgctgg cggcaaagct ggcactggaa
     2161 gcttcggcaa aatatcgcgt cggcacgggc acctctttga tgcgaaatcc caggtgaacc
     2221 acctggacca acatttcctg gtcgaaaaca aagttatccg aattaagcag gaacggcact
     2281 tcttccaaaa cccgccggga gaatgcccgg taacccgtgt gatattcggc aagcgactga
     2341 cggagcactt ggttctgaat accggtcaag aagcgattac cgaggtattt ccaccatggc
     2401 attccttgtt taaccacctc gccggcaagc aagcgcgaac ctagaaccac atcggcgtcg
     2461 ccgttcttga tcggggcaat gacattgggc aacaacgacg gatcgtattg atagtcagga
     2521 tgaagcatga cgacaatttc ggcgccagcc ttcagggcct cggtatagca ggttttctgg
     2581 ttgcctccat agccgaagtt acgggtgtga acgaacactt ccaaattgag ctctttggcg
     2641 atttttaacg tctcgtcccg gctaccatcg tcgacaagaa tcacctgata cacgtcatcc
     2701 ttgggaatgg cgtcgtaggt tatgcgcaga gtcttggccg cattgtacgc cggcatgacg
     2761 acgatcacct tcggctggtt aagagcgact gccatggcgt ttcagatatc taattgcgta
     2821 ttaaagcagg gggcggccaa ccccgtgtcg caacttgacg gcgtccaggc gcttcgactc
     2881 tcggaccacc cgctcgatga tccgacgccg taactgccgg ctggccgatt ctcgatttaa
     2941 ataggcttgc agtacccgca atttctgaac gttgcgcaga tatctgccga gcgtacgatt
     3001 cagttgaacc aaattcttga ttcttcggct ttccgacagg gcgcgatatt gacgcacacc
     3061 ctctaaatcg agcaaaaaca aggcagtcct ctcgctatcg gttatcggta ccgcgagaat
     3121 gttagcgtcc ttcagatcgt tatggtagac gccctggctg tggagtgcgt gaaacagcgt
     3181 gcccagggcc gtcagaaacg cacgccgact cctaggcccc tcaggagtcg gccgagaact
     3241 caggatttct cgccataggg agtccacggt cacgcctcct tgaatctcct cggaaagaaa
     3301 aaaactccag gtgaccgcgc cccatatccg ttcttcgacg gcggcgatcg gttttgccgt
     3361 tgcgataccg ttacgggtca aaatagcggc accgcgtagt gatcttatcc ctcccgaagg
     3421 gctgaatatc gatcctaagc gatgacgcaa cgaaaaggta ttgtagcgtt tgacataaat
     3481 gcgccgccgc gcgccgccaa gctcgagcgc cactctcgcc accttgatct tgcgttgatc
     3541 cttgataact tcgcagccgg gaagatcgaa aagctcatcg ggttttttta acagcgccag
     3601 caccgcggga tgggcatgat tagcatcgag ccatatccga catctcccgg agcgatgtaa
     3661 aataaaatta ggatacacgg atggcgggcg gcccatgaca aacttcgttg agtagggctt
     3721 caagccggtg gaaatggcta ttccacgagt actgttcacc gagctggcgc gccgcatcgc
     3781 gaaactccgg cttcgcacag tgttcgagca tgttgcaaag ccgagcttcg agttgtgtgg
     3841 aatccccggc atcttcgacg actccttgtc tcaagcagcc ggtcaatacg gcgccggcgc
     3901 ccacttcctg gctgaccact accggcacac cggatgccag tgattcaagc acgacattgc
     3961 cgaacgcctc ctgcaatgcc ggcagggcaa ccagatccgc catggcgtaa taggtctcaa
     4021 tgtcatcctg ccggccgaca aagagaatgc gctctccagc gaccgacttc gcccaatttt
     4081 ggtaatggcc catgcgccca tcgtccccgg caaccattaa gtagacgtgg gtcaatggcg
     4141 gacgattcca aattgcgatc agacggtcca agcccttgcg cgcaaatccg ctgccgacaa
     4201 acaagacgag tggcgactgg ttgggaactt tccagcgcgc gcgctggatt tcgcggacct
     4261 gcgtgcgccg agccggatga aagcgctgcg cgtcgacgcc attgtaaaga acctcaatct
     4321 tgtcgttctc gacccggtaa tgtctctcaa tgtcgcactt gacctcatcg gacactgcga
     4381 tcaccttctt gaaatgcccg tcggaaaact gttttctctc tatccgtaac agactctggt
     4441 gatacggact caggcgttgg cgcagccgcc gccatgcccc ccgttgttgt cccattcgca
     4501 atagaaagcc gcgatgcgtg ccgccgccac tgcgtaagat atcctgggag agaagacggc
     4561 caaagctgac cacgacatcg catcgctgat tcttgataat acgcggcgct gccagggcga
     4621 agctccacaa tcgcaacgtg cgtcccaatc gaaccaccgg gatggtatga ccgatcgtcc
     4681 cacgcggcgt cggcactcca tactcgctgc agaacaaatg cacttgatgg ccgcgatcgc
     4741 gcaagccttc cgcggtttga tagagatccc gttcggtgcc gcccttgaga tcaagccgtt
     4801 tatgaactag ggcaattttc aaacgtgagt tttcttggct ttaacgacga tattgtccga
     4861 caacaaaagc gcgggatgaa aaaggtagga gaggatttgc caattatagc gccgctgcag
     4921 tgggtctttc tcggcgtgta tcactgtcat aaaactaaaa agccacatga atggccaaaa
     4981 gattatcatg aacaagcgcg cggcaaattt tacccgagtt ttggaaagtc cagcaacttc
     5041 gaatccataa tgaaatagta ggtaataaag ctcatagaaa tgtaacaagt aaatattcgg
     5101 cgcttgacct ggtttcctcg aatagtgcgc tggccgaacc cggccgcgca aaaaaccggt
     5161 aaacaaaaac cgcactcgcg acattagatt aagcacattg ggagtgctga tgagcgctac
     5221 tccccccggc ttgaggactc tggccagttc gcgaatcaat tgcggctgat tttcgatatg
     5281 ctctatcact tcgaccaaat ctatcgcgtc aaaactcgcc gtgcgaaacg gcaagaacgc
     5341 attgaggtcg accccggcga cacgctcgat tcccggcgtc atcgacggtg gcggaggata
     5401 gtccgtggca gttactttat agcccagtcg agccaacatt tcagcggtat ctccgccacc
     5461 gcagctcaaa tccaatattc tctttccaac cgggtcctcc gttgaccagg ctgaagatac
     5521 tgcgtcaaaa atagcgttgt taggcatttc gagctgctca agtccttgcg gttagcgggg
     5581 tgtaaagtta ctttctaatt ctcccttggc agtccgtatt cggcccccgg cggaacaata
     5641 tttagccgtt ttgggcactg gtcgtctccg gcaccaagca catcagcttt cggaaacttc
     5701 cgctcggatc tacgccatca cattcgttac ggcgctggcg ggtgcgcgcc tcaagccagc
     5761 gggcgaaccg gcgatcgcgc gccgtcaatc tccgcacgcc gagataagcg tgctgaaaac
     5821 gcagtcgatc ttgcagcgta atatgcgcca acggcaatcg gttgagctgc accaagttgc
     5881 gcttccttaa gttaccggtc agccatggcg aatagtgcc
//
LOCUS       DASQKS010000250         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1385918, whole genome shotgun sequence.
ACCESSION   DASQKS010000250 DASQKS010000000
VERSION     DASQKS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385918"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VE170_06485"
     CDS             complement(<1..530)
                     /locus_tag="VE170_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15119.1"
                     /translation="MGVTKFGSHYLPTYVPDLDGSVSEFYQKMFGQMEEMDRLGYDHI
                     WVTEHHFAHYGGDLPHPPTFMSAIARTTKRIRLGVAIGVLPLHNPIDLAESYAMVDVI
                     SNGRVDFGVGKGSEAHEYKKYRVDQKEATGRMYEGVEVMLQAWSDKPVNFKGEFFNYE
                     NVPVFPKPVQRPHPRF"
     gene            716..1690
                     /locus_tag="VE170_06490"
     CDS             716..1690
                     /locus_tag="VE170_06490"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYJ15120.1"
                     /translation="MIHTRICDLLGIAHPIVLGGMGGATNAPLVAAVSNAGAFGTLGT
                     SALNAATLDNEIGAIHERTDKPFGINHLLFQIQEDMFAVTLRARPALVAFAWARKQQN
                     LRDYFQRAHDAGCKVMHMAGEVAEALRAADAGADVLVAQGTEAGGHVGWMASLPLLPM
                     IVKAVAPLPVLGAGGIADGRGLAAALALGADGVLLGTRFLATPEAPIHANFKQAIVKS
                     DGHDTVLTEIPDLASQRVWPGAMSRAQRNLFIERWSGREWAIRQNAAEIGKQVAAARV
                     SGDIDNATLSFGQDAGLIDSIKTVNQVVQDVVAEAEEILRVRLPALLC"
     gene            1808..2206
                     /locus_tag="VE170_06495"
     CDS             1808..2206
                     /locus_tag="VE170_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HYJ15121.1"
                     /translation="MEFTADRFEKKSWRQRAKELKTETYALYLAVRDPRVPWYAKVSA
                     LCVVAYALSPIDIIPDFIPVLGYLDDLILLPIGIALTIKMIPALVLAECRDKAQAHIA
                     AGSLLGWAGAAVVIMIWLAAGAGLFHYFAR"
BASE COUNT          502 a          664 c          617 g          485 t
ORIGIN      
        1 caaaagcgcg gatgcggccg ctgcaccggt ttgggaaaca ccgggacgtt ttcataattg
       61 aaaaactcgc ctttgaaatt caccggcttg tccgaccacg cttgcagcat gacctcgacg
      121 ccttcataca tgcgcccggt cgcttccttc tgatcgacgc ggtatttttt atattcgtga
      181 gcttcgctgc ccttgccgac gccgaaatcc acccgtccat tggaaatcac atcaaccatc
      241 gcataggact cggcaagatc gatcggatta tgcagaggca atacgccaat cgccacaccg
      301 aggcgaattc gtttggtcgt gcgcgcgatc gccgacatga acgtcggcgg atggggcaga
      361 tcgccgccgt agtgggcgaa gtgatgctcg gtcacccaaa tatgatcgta accaagccgg
      421 tccatctctt ccatttggcc gaacatcttc tggtaaaact cggacaccga gccgtcgaga
      481 tccggcacgt aggtcgggag ataatgcgat ccaaacttgg tcacgcccat ggcttgataa
      541 acctccagga aatttcactc aacatataag aggctcgcgg ccaagaagtc aacggaccga
      601 gctaactcga atccccaaaa ataatcttca cgccaagtcc ttttggcgcc gatttacatc
      661 gcgatgccat tggcatagga cagatattca gcgcttgatt gaaggaggag agatcatgat
      721 tcacacgcgc atttgcgatc tgctcggcat cgcccatccc atcgtgctcg gcggcatggg
      781 cggcgccacc aacgcgccac tggtcgccgc ggtgtctaac gccggcgcct tcggcaccct
      841 gggcacttcg gccttaaacg cggcaacctt ggacaatgag atcggcgcta ttcatgagcg
      901 aaccgacaaa cccttcggta tcaatcacct tttgtttcag atccaggaag acatgttcgc
      961 ggtgacactg cgcgcccgcc cggcgctcgt cgccttcgcc tgggcgcgca aacaacaaaa
     1021 cttgcgcgac tactttcagc gtgcccacga cgccggttgc aaagtcatgc atatggccgg
     1081 tgaagttgcc gaagcgctgc gcgccgccga cgccggcgcc gatgttctgg tcgcgcaagg
     1141 taccgaagcc ggcggccatg tcggttggat ggcatcgttg cccctattac cgatgatagt
     1201 aaaagcggtg gcaccgttac cggtgcttgg tgccggcggc atcgccgatg gccgcggtct
     1261 cgccgccgcc ctcgcccttg gcgccgacgg cgtgttgctc ggcacccgct tcttggccac
     1321 gcccgaagca ccgatccatg cgaacttcaa acaagccatc gtcaaaagcg acggtcacga
     1381 caccgtactc accgaaattc ccgacctcgc aagccagcgc gtctggcctg gggccatgtc
     1441 gcgtgcccag cgcaatttat ttatcgaacg ttggtccggc cgcgagtggg cgatccgcca
     1501 gaacgccgcg gagatcggca agcaggtcgc cgctgcccgc gtctccggcg acatcgacaa
     1561 cgcaactcta tccttcggcc aggacgccgg tttgatcgac tcgatcaaaa ccgtaaacca
     1621 ggtggttcag gatgtcgtcg ccgaggcaga agaaattctg cgggtccggc tgcccgctct
     1681 tttgtgttag taaaccaagc ggagtgacca gctcgacagc ggttgcaaat cgatcacgct
     1741 gaaaactgtg caaaagcgct gcgcgaccaa tagatacgct caataaagcg aaggggcata
     1801 cgggaagttg gaattcacgg ctgatcgatt cgaaaaaaag agttggagac aacgggcaaa
     1861 ggaattaaaa accgaaacct atgcccttta tttagcggtt agagatccaa gagtgccatg
     1921 gtatgcgaaa gtctccgccc tgtgtgttgt cgcttatgca ttgagtccga tagacatcat
     1981 tcccgacttc attcctgttt taggatatct tgacgatctt atactgcttc cgatcggtat
     2041 agcgcttacg attaaaatga tacccgctct ggtgttagcg gaatgccggg acaaagcgca
     2101 ggcgcacatc gctgcaggca gtcttttagg ttgggctgga gccgctgtcg ttatcatgat
     2161 ttggttggcg gctggagcgg ggctttttca ctatttcgcg cgttgaagag ccggtcgatt
     2221 tacccgcagt taatcgcccg ccatcttatt catctcaagc gttgctgc
//
LOCUS       DASQKS010000251        10531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5586308, whole genome shotgun sequence.
ACCESSION   DASQKS010000251 DASQKS010000000
VERSION     DASQKS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5586308"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VE170_06500"
     CDS             complement(<1..797)
                     /locus_tag="VE170_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15122.1"
                     /translation="MQKIVPHLWFDKEAKEAAEFYTSTFPDVKLTNITTIHDTPSGDC
                     DVVSFELWGYSFMAISAGPLFKFNPSVSFMVNFDPSQDKDAKMRIDEVWGKLSAGGNI
                     LMPLDKYPFSERYGWVQDKYGLSWQLILTNPEGEERPNIIPFLLFVGDVYAKAEEASD
                     FYLSIFTRSKRGAIVRYGAGQAPDKEGAVMFTDFMLKGQWFAAMDSAHQHNFKFNEAV
                     SLTVNCDTQEDIDYYWKKLSAVPEAEQCGWLKDKFGLSWQIVPTELD"
     gene            complement(912..1349)
                     /locus_tag="VE170_06505"
     CDS             complement(912..1349)
                     /locus_tag="VE170_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ15123.1"
                     /translation="MSLFMTKQEREAFLADVHVGVISIAEIGRGPLTVPIWYNYEAGG
                     EIRIMTGRESRKGRLLAHAGRFSLCAQTESPPYKYVSVEGPIVSTEAADIERDLRPLA
                     RRYLGKESGDRYVEETRNLPTHSDNVLIRMRPERWLTTDYSKE"
     gene            2288..2974
                     /locus_tag="VE170_06510"
     CDS             2288..2974
                     /locus_tag="VE170_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15124.1"
                     /translation="MGADLSSVRFALLGLFLFLAALTHLPAAESPAALTARESDTPLY
                     GRQDLETEPIMRLQKGEALMPMAESVGQEIWYMVRTKQGQIGWVRAVDVTVSSRVKDS
                     FKETDAASSNWGAVSEDGKAFGGSYTVDPSSTGSAARGFWTLRDAGGATSLRGAWSAQ
                     MHRTGWNGTWRAALEGRGGDYTGSWSAELPAARALRFTDMFEMAAKDAIKGLWTGANQ
                     SGSWSIKTFK"
     gene            3161..4798
                     /locus_tag="VE170_06515"
     CDS             3161..4798
                     /locus_tag="VE170_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HYJ15125.1"
                     /translation="MPRSYNRFVLLLGALGLLLLLMSLLYMVGMYFLENKPRGFWQAL
                     QWAAGTVSTTGYGGDISWQHPLMVIYVVIAQFIGVMLLFLVFPIYLIPFLEDRFETKL
                     PKESVNAKNHVVIFDYGPAVATLITELKQTNIPTLLIDEDEDNARHLLEQGHNVIYGN
                     LDEGVLGKANLGAARALIVNSSDDRNAATILAARQLGYNGEILALVEDPYHRQPIILA
                     GASSAYTPRHVLGAALAARASQKVSPTVAGIQHLGHKLQVAEARITRDSPLAGKTLAE
                     AALGQHAGAGVTVIGQWVGGKLITPATPAMRLEAGGILVLVGNDDGIRSFLDLCAGAR
                     RLRREGPFLIAGGGEVGRKVAELLNDCGEETFIIDSQPGASVNLVGNVLDTQILKQAG
                     LENVQAIILALSADSTTLFSTVIVKDLAPDVPVIARVNRAENVERIYAAGADFALSIS
                     QVSGQLLAWRLLGKESIAVDAELRVIKVSTRGLENLHPRQNDLRERTGCTIVAVERGE
                     ELLVEFPTDFKFAANDAVYICGSAEATQKFYQLFPQE"
     gene            complement(4885..5943)
                     /locus_tag="VE170_06520"
     CDS             complement(4885..5943)
                     /locus_tag="VE170_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15126.1"
                     /translation="MKRNVSLVFTVMIGLFAARSALAGQAGFDEKSVANFYRGKTVTI
                     VVGHSAGGGFDRYARAIARHLGKYIPGNPAILVNNMVGAGTMVSANYTYNQAPRDGTV
                     VNSFDGGIWPSQLYGASAAQFDLTKFNYIGAPDIFKYIMVVTKKSGITRMEDFMSGAK
                     EVVVGAVPNTSIQHSATFLKEVLGGKVKLVTGFKGTAEIRLAMQSGEVNSVITGWETL
                     SVSNFKDFESGEWLILSQWVDEPLTDLPQKNVPSIYQYARNDEERQLLKLGLVKPNSY
                     ARPYAMPPGVPLERVRAVEAGFLKSLKDPEFVAEAEKTRMTVSPISGSDLQMMFAEGM
                     SMPAALKAKLKPILLPKS"
     gene            complement(6069..6710)
                     /locus_tag="VE170_06525"
     CDS             complement(6069..6710)
                     /locus_tag="VE170_06525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HYJ15127.1"
                     /translation="MTANSTRPADYWPISTNNVALILVDMQNIWVHPRGARYLPSSED
                     IVPKIQELLRFCHASRVPVIYLHTTKRRDLADVGIFADVKPQTHDADDEWSNFEGTPG
                     AEIYEPVKPTTDDILVKKFRYSGFYGTQLENLLRALGRDTIAITGVATNVCCDSTARD
                     GAMRDFKVLFLSDCNASFSQAEQEATLGNFDKHFGVVMDSQTLLGKLGAEKQA"
     gene            6852..7490
                     /gene="pssA"
                     /locus_tag="VE170_06530"
     CDS             6852..7490
                     /gene="pssA"
                     /locus_tag="VE170_06530"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HYJ15128.1"
                     /translation="MARRKPEKKRVHFSMLRDYRAADLITLANAGAGMAAVLVMMSYL
                     ITAEDWRVYLSLALVLIALLLDIADGKVARRRGEYSPFGQELDSLADIVSFGVAPAAL
                     GYGLGLRGGLDIIILIFFVACGISRLARYNVTASQLSDASGKVKYFEGTPIPSSLFLV
                     ALLALCFYFGRTGAHFFFGTIAIAGLDLHPFALLFFANGCTMISKTLKIPKL"
     gene            7577..8497
                     /locus_tag="VE170_06535"
     CDS             7577..8497
                     /locus_tag="VE170_06535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15129.1"
                     /translation="MAARLAAQEKIRVAISNFSASYIPIVLANKRGYYAEESMGVEII
                     LMAGLTGTRALIGNSVEFGSASNPTAAVQGAKLKILMVFNDKPPGSFMAQPAYKSIAE
                     LRGKRVGGSTIGSLDYGWLKEVLPKFGLQLEKDVNFVPVGSTSARYVALRAGSIDAAS
                     LSPPSSLLAHSLGYSTFFNFADHLEDIQASIVASDDRLGRQGELVRRFMRATVKGQRL
                     YQANRQESITAIMEFTRQNDRDLMSRVYDDHMKTVAKDGTISERLQRIVIERSKRFTG
                     VTRDVRPEEIFDFSFLRRAQAEVTQSGWNP"
     gene            complement(8558..9457)
                     /locus_tag="VE170_06540"
     CDS             complement(8558..9457)
                     /locus_tag="VE170_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ15130.1"
                     /translation="MAIRMGTSELGGTFYTQGQAIAEIFNRGRGVGDRCVVETSGASI
                     HNANQLDAGTLEFAFMASNWIGRAKDATAPFTKNIAIRMVAPANAGPMCFVRLAGVPI
                     ASVADFAGKRVAVGAKGSGMEQHVQTIFKVLGISFDSFTPVHMGFAEGADALIAGQID
                     VQFHPPIPNAVLTDLSRRADIRVIPYAAEQRDKLFAEVPFYRPVTVERNAFRGVVEDV
                     IQVGVINVIVSHERVAEEIVYAMAKTIADNLDQLPQMNPLFKGLKNLFEPLRNKGAAA
                     FEFGGVPLHPGAVRAYKEAGWLG"
     gene            9598..10248
                     /locus_tag="VE170_06545"
     CDS             9598..10248
                     /locus_tag="VE170_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ15131.1"
                     /translation="MFVEKWMTANPDTVAPEETISAVALEMSRRKVRHFPVCEPSRTG
                     RRMVGIVAKFDIARGFPNDLNPFSVEVTEDSVPKPVSSVMTKKVITVLPDCPIEEAAR
                     LLRTHKIGCLPVLKDNQIVGIIAQSDLFEAFIDMTAAKSGGTRIFIESDFDDSPVPTV
                     LDFCRLNRVEILSLMSFHENRLRRRDMSIFRFAGRLPKGFIPAMAAAGYRVVSVGE"
     gene            10345..>10531
                     /locus_tag="VE170_06550"
     CDS             10345..>10531
                     /locus_tag="VE170_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15132.1"
                     /translation="MPFLDRDGSEIYYETHGDGPALLFCSVTGLDHQAWKFHQVPEFS
                     RDHKVILFDYRGTGKSSK"
BASE COUNT         2274 a         3056 c         2871 g         2330 t
ORIGIN      
        1 tcatccaatt cggtcggaac aatctgccac gagaggccaa atttgtcctt gagccagccg
       61 cactgctcgg cttcaggaac ggccgataac ttcttccagt aataatcaat gtcttcctgg
      121 gtgtcacaat tcaccgttaa tgacacagcc tcgttgaatt tgaaattgtg ttggtgcgcg
      181 ctgtccatcg cggcgaacca ctgaccttta agcatgaaat cagtgaacat gacggcccct
      241 tctttatcgg gcgcctgtcc agcgccatag cggacaatag cgccgcgttt ggagcgcgta
      301 aatatcgaga gatagaagtc agaagcttct tccgctttgg cgtaaacatc gccgacgaac
      361 agaagaaacg gaataatgtt tggtcgctcc tcgccttcgg gatttgtcag gatgagctgc
      421 caggacaggc catatttatc ctgtacccag ccgtagcgtt cgctgaatgg atatttatcg
      481 agcggcatta gtatattacc gcccgccgat agttttcccc acacttcatc gatccgcatc
      541 ttggcatctt tgtcttgcga cggatcgaaa ttgaccatga aggatactga tggattgaac
      601 ttaaataggg ggcccgcgct tattgccata aaggagtatc cccacagctc aaatgagacg
      661 acatcgcaat ctccggatgg cgtgtcatgg attgtggtta tgttcgttag ttttacgtcc
      721 ggaaaagtag aagtgtagaa ctcagctgcc tctttggcct ctttatcgaa ccataaatgt
      781 ggaacgattt tttgcatagg atatcctttc agtgatcttg agcacgcaca cctattccac
      841 ttggccaacg tattttttgc agtaagcgct gcggttaagc tcgtcaaaag actttgctac
      901 atcgatgact gctattcctt ggagtaatcg gtggttagcc agcgttcggg tctcatgcgg
      961 atgagcacat tgtcgctgtg cgtcggtaag tttcgcgtct cctcgacata gcggtcgcca
     1021 ctttctttgc cgagataacg gcgcgcgagc gggcgcagat cgcgctcgat atccgccgcc
     1081 tcagtggaaa cgatgggacc ctctacactg acatacttgt acggcgggct ttcggtctgc
     1141 gcacagagac tgaatctgcc ggcgtgtgcg agcagacggc cttttcgcga ttccctaccg
     1201 gtcatgatcc gaatctctcc tcccgcttca tagttgtacc agatcggcac tgtgagcggt
     1261 cctcgtccta tctcggcaat gctgattacg ccaacgtgaa catcggccag aaacgcctcg
     1321 cgctcttgtt tagtcatgaa cagcgacatt ttgtcctccg agtatcaaaa atcgttcgcg
     1381 acacagctca gcaggcgcga tcctaagtgt ttcgagcaga tgcgatggct tcacctgctc
     1441 tactcttttc gctgatcgga tcgctccacg gcggtgaccc tgtcacggtt cttcgtggca
     1501 tagcggagtg atcgtaggga cctgctgccg cgaacagagg tgggtcagat aaggtgtgcc
     1561 acggccgccg tacggacggg tgagtttctg acgaggcgca tcacgttgtc actgacctga
     1621 ccctgccatt aatacgcagg aaggtcgcag aactaagatg ccgaagtgca acttgtgggg
     1681 tcagcaacgc acgccgttac tttactccaa ccactcgcca atccattacc aatgtcagtg
     1741 ttcttaatgg cgacccaaaa aaccagcgcc accaaaacca cgatcaaagt gtactcaacg
     1801 agaccttgac catcttcttc gactaacaaa cgtttcaata gctgcatcta aattcacctc
     1861 ttttctttca gatttagagg aggtagcccg gcatgactcg taaccatttc cctatcggcc
     1921 tccgtcgaga cgtaacaaga gaacaccggg aaacttgccg gtggccgcac atagtccatc
     1981 ctaacactcg tcagctccaa ggaaataggc ttaccgttac agcgaaatgt cccagatgat
     2041 tggtaccacc gtatgaacga aatttgtctt ctatatcaat cagtttcagc caattttcgt
     2101 catctttttt aataaatttg gtcataatat ttcagatata tctgcaacag atatctcccc
     2161 tagcaggact gactgaacga agccccggcc aagtcgtcag tgacggctac agtggaaata
     2221 gactaaactg tcgattcacg accacagcgt cgtgttatta tcagcagacc tacgggagaa
     2281 ttttactatg ggtgcagatc tcagcagcgt acgctttgcc ttacttggct tatttctttt
     2341 cttggcggca ttaactcatc tgcccgccgc cgaatctccc gccgcgctca cggcgcgcga
     2401 gtcggacaca ccgctttatg ggcggcagga tctggaaacc gagccgatca tgcgattgca
     2461 aaaaggcgag gcgctgatgc ctatggcgga gagcgtcggc caggaaatct ggtacatggt
     2521 gcgcaccaag caaggccaga tcggctgggt gcgcgcggtg gacgtcacgg tcagcagtcg
     2581 ggtcaaggac tcgttcaaag aaacagatgc ggcgtcgtcg aattggggcg cggtgagcga
     2641 agacggtaag gctttcggcg gcagttatac cgtcgatccg agctcgacgg gtagtgctgc
     2701 tcgcggcttt tggacattgc gcgatgccgg cggcgcaacc tcgctgcgcg gcgcctggtc
     2761 ggcgcagatg catcgcaccg gctggaatgg cacctggcgc gccgcgctcg aaggccgtgg
     2821 cggggattac accggcagct ggtccgccga gctgccggca gcacgcgctc tacgctttac
     2881 cgatatgttt gagatggcgg ccaaagatgc gatcaaaggc ttatggaccg gggccaatca
     2941 atccggcagc tggtcgatca agacgttcaa gtagcactca cggcgtttct ttcaccacgg
     3001 cggtgtgtgc gaaagtttgt tgaagaatcc tgccacgcca tgttaagcaa ggctacggtt
     3061 tacgcatggc catagaaaac tgcatccctg aatcatcgca ccgccgttta caatcaccaa
     3121 caactaaccg ttcgacggca cgacgcggga cccggcgcta atgccgcgct cctataatcg
     3181 ctttgtcctc ctgctcggtg cgctcggctt attgctgctg ctcatgtcgc tgctctacat
     3241 ggtcggcatg tattttctcg agaataagcc gagagggttc tggcaggcgc tgcaatgggc
     3301 cgccggcacg gtatcgacca ccggttatgg cggcgatatt tcttggcagc atccgttgat
     3361 ggttatctac gtggtcattg cacagttcat cggtgtgatg ctgctcttcc tggtttttcc
     3421 aatttatctg attccgttcc tcgaggatcg gttcgagacc aagctgccga aggaatcggt
     3481 caatgccaag aatcacgtgg tgatcttcga ctacggtccg gcggtggcca ccttgattac
     3541 ggagctgaag caaaccaata ttcccactct gcttatcgac gaagacgaag acaacgcgcg
     3601 ccacttactc gagcaggggc acaacgtcat ttatggtaat ctcgacgaag gcgtgctcgg
     3661 caaagccaat ctcggcgcgg cacgcgcgct gattgtcaat agcagtgacg accgcaacgc
     3721 tgcgacgatc ctggcggcgc gccagcttgg ctacaacggc gagattctcg ccctggtcga
     3781 agacccgtac caccggcaac cgatcattct cgccggcgcc agcagcgcct acacgccgcg
     3841 ccatgtcctc ggcgcggcac tagcggcgcg cgccagccag aaagtcagcc cgacggtcgc
     3901 gggcatccaa cacctcggtc acaagctcca agtcgccgaa gcccgcatca cccgcgatag
     3961 cccgctcgct ggcaaaactt tggcggaggc cgcgctcggc cagcacgccg gcgccggcgt
     4021 caccgtcatc ggccagtggg tcggcggcaa actaatcacg ccagcaacgc ccgccatgcg
     4081 ccttgaagcc ggcggtatcc tcgtcctggt cggcaatgac gacggcatcc gcagtttctt
     4141 ggatttgtgc gccggtgcgc gccggctgcg gcgcgagggg ccgtttttga ttgccggcgg
     4201 cggcgaagtc gggcgtaaag tcgctgagtt gctcaacgat tgcggtgaag agacgttcat
     4261 catcgatagc cagcccggcg ccagcgtaaa tctcgtcggc aacgtcctcg acacgcagat
     4321 cctcaaacag gcaggtttag agaatgttca agcgatcatt ctcgctctca gcgccgactc
     4381 gacgacgttg ttcagcaccg tcatcgtcaa agacttggcg cccgacgtgc cagtcatcgc
     4441 ccgcgttaat cgcgccgaaa atgtcgaacg catttacgcc gccggcgccg attttgccct
     4501 gtcgatcagt caagtatcgg ggcagctgct cgcgtggcgg ttgcttggca aagaatcgat
     4561 agcggtggat gccgagctgc gggtcataaa agtttcaacc cgcggactgg agaatctgca
     4621 tcccaggcaa aatgacctgc gcgaaagaac tggctgcacc atagtcgcgg tggagcgcgg
     4681 cgaagagttg ctggtggaat ttcccacaga tttcaaattt gcagccaacg acgcggtata
     4741 tatctgcggc agcgccgaag cgactcagaa gttttatcaa ttattcccgc aggaatagga
     4801 ctcgaaggat tggccgcaaa gcacgcaaag ttcgcaaaaa aggaaattga aatcgtcagg
     4861 caacccccag tggttgttgc ctgattaact cttgggcaac aggatcggct tgagcttggc
     4921 tttgagcgcg gcgggcatgg acattccttc ggcgaacatc atttgcaaat cactgccgga
     4981 gatcgggctc acggtcatgc gggttttctc ggcttcggcg acgaactccg gatcttttaa
     5041 acttttcaaa aatcccgctt ccaccgcgcg cacgcgctcc agcggcacgc ccggcggcat
     5101 cgcgtaggga cgcgcgtagc tattgggttt gaccagtccc aacttgagca actggcgctc
     5161 ctcgtcgttg cgcgcatatt gatagatcga cggcacgttt ttctgcggca aatcggtcaa
     5221 cggctcatcc acccactggg atagaatcag ccactcgccg ctctcgaagt ctttgaagtt
     5281 ggaaacgctc aaagtctccc agccggtgat cacgctgttg acttcgcccg actgcatcgc
     5341 caggcggatt tccgcggtgc ctttgaagcc ggtcaccaat ttcactttgc cgccaagcac
     5401 ctctttaaga aaggtcgccg agtgctgaat gctggtatta ggtacggcgc cgaccaccac
     5461 ctcttttgcc cccgacatga aatcttccat cctggtaatg cccgacttct tggtgaccac
     5521 catgatgtat ttgaaaatat ccggcgcgcc gatgtagttg aacttggtta gatcgaattg
     5581 cgcagccgag gcgccgtaga gctgcgaagg ccagatgccg ccgtcaaaac tgttgaccac
     5641 ggtgccgtca cgcggcgctt gattataagt gtagttggcg gaaaccatcg tcccggcgcc
     5701 caccatgttg ttgaccagta tggccggatt accggggata tatttaccca gatggcgcgc
     5761 gatcgcgcgg gcgtagcggt cgaatccgcc tcccgctgaa tggccgacga cgatagtcac
     5821 cgtcttgccg cggtagaaat tagcgacaga tttctcgtcg aatcccgcct ggccggcaag
     5881 cgcggagcgc gcggcaaata acccgatcat cacggtgaac accaacgaaa cattgcgttt
     5941 cacgataaac ctccagatca cgattgcctg cactggagta atggagtgtt gggtaaagag
     6001 ttgtccggaa ccccgcaccc caatgctcca ctcctcaaac actccaactc ttttactcca
     6061 tcgctccatt acgcctgttt ttccgcgccg agcttcccca gcagcgtctg cgaatccatc
     6121 acgacgccga agtgcttgtc gaagttaccg agcgtcgcct cctgctcggc ttgagagaag
     6181 gaagcattgc aatccgataa aaacagcact ttgaaatcgc gcatggcgcc gtcccgcgcc
     6241 gtgctgtcgc agcaaacgtt ggtcgccacg ccggtgatcg cgatggtgtc gcgccccagg
     6301 gcgcgcagaa gattttccaa ctgggtgccg taaaagccgc tgtagcgaaa cttcttgacc
     6361 aagatatcgt ccgtcgtagg tttcaccggc tcgtaaattt ccgcacccgg cgtgccttcg
     6421 aaattgctcc actcatcgtc ggcatcatgg gtttgcggct tgacgtcggc gaagatcccg
     6481 acatcggcga gatcgcgccg cttggtagtg tgcagataga taaccggcac tctgctggcg
     6541 tggcagaagc gcaatagctc ttgaatcttc ggcacgatat cctcggacga cggcaagtag
     6601 cgcgcgccgc gcggatgcac ccagatattt tgcatgtcga cgaggataag cgcgacattg
     6661 ttcgtcgaga tcggccaata atcggcgggc cgggttgaat tcgctgtcat gttccctcct
     6721 gcaattaacc agcgatcaca ctcggcgcat ctaagcagcg ccgcggcagc ctgtcaatgc
     6781 gccacaaggc gattcagtag acacttccgc aagggctatg ctagcgtgcc gggtcaagac
     6841 gtaaaaacac catggctcgt cgcaaaccgg aaaaaaaacg cgttcacttc tccatgctgc
     6901 gcgattatcg cgccgcagat ctcatcaccc tggccaacgc cggcgccggc atggccgctg
     6961 tcttggtgat gatgagctac ttgatcactg cggaagactg gcgcgtctac ctatcgctag
     7021 ccctggtgct gatcgcctta ttgctcgaca tcgccgatgg caaagtggcc cgccgacgcg
     7081 gcgagtactc gcccttcggc caggaattgg actctctcgc cgacatcgtc agcttcggcg
     7141 tcgcgcccgc ggcgctgggc tatggcttgg gactgcgcgg cggactcgat atcattattc
     7201 tgatcttctt cgtcgcctgc ggcatcagcc gtctggcgcg ctacaacgtc actgcgagtc
     7261 aattgagcga cgccagcggc aaagtgaaat atttcgaggg cacgccgatc ccgtcgagcc
     7321 tattcctggt ggcactgctg gcgctgtgct tttacttcgg acgcaccggt gcgcattttt
     7381 tcttcgggac aatcgccatc gccggcctcg acttgcatcc gttcgctctg ctgttttttg
     7441 ccaacggctg taccatgatc agcaaaacgt tgaaaattcc caagctctaa cgctggcgaa
     7501 ccatcatgac gaggatacta ctgtgatgcg gcgaaaagtg attctttcat cggctctgct
     7561 attcgcaata ccaatcatgg ccgcacggct tgccgcccag gaaaagatcc gggtcgcgat
     7621 ctcaaatttt tccgcgtcct atattcccat tgtgctggcc aataagcgcg gttactacgc
     7681 cgaggaaagc atgggagtcg agatcatctt gatggcggga ctcaccggca ccagggcgct
     7741 catcggcaac agcgtggaat tcggctcggc gagtaatccg accgcggcgg tgcaaggcgc
     7801 gaagttaaaa atcctcatgg tgtttaacga caagccacca ggcagtttca tggcgcaacc
     7861 ggcctacaaa agcatcgccg agttgcgcgg caaacgggtc ggcgggtcga ccatcggcag
     7921 tttggactac ggttggttga aagaggtgct gcccaagttt ggcttgcagc tggaaaaaga
     7981 cgtgaatttc gtgccggtgg gctcgaccag cgcgcgctac gtggcgctcc gagccggcag
     8041 catcgacgca gcgtcactga gcccaccgtc gagtctcttg gctcattcat tgggttactc
     8101 gacgttcttc aatttcgccg atcacttgga agacatccaa gcgtccatcg tcgccagcga
     8161 cgaccggctg gggcgccaag gcgagctagt gcgccgcttt atgcgcgcca ccgttaaggg
     8221 ccagcgcctt taccaagcca atcggcaaga gagtataacg gcgatcatgg agttcacgcg
     8281 gcagaacgac cgcgatctca tgtcacgggt ctacgatgat catatgaaga ccgtcgccaa
     8341 ggatggcacc atcagcgaac ggctccaaag aatcgtcatc gagcgcagta agcggttcac
     8401 cggcgtgact cgagatgtgc ggcctgaaga gattttcgat ttttcttttt tacgccgggc
     8461 gcaggccgaa gtcactcaga gcgggtggaa tccgtaaaga aattcgatca cagctggggt
     8521 gaaccgatgt ggtttgcgat cttcgcggcc gaaactatta acccaaccat cccgcttctt
     8581 tatacgctcg taccgcgccg ggatgcagcg gcacgccgcc gaactcgaag gcggcggcgc
     8641 ctttattgcg cagcggctcg aagagatttt ttagcccttt gaatagcgga ttcatttgcg
     8701 gcagctggtc taaattatcg gcgatggttt tcgccatggc gtagacaatt tcttcggcga
     8761 ccctctcatg gctgacgatg acgttgatga cacccacctg aatgacatct tctacaacac
     8821 cgcgaaatgc gtttcgctca actgtgaccg gacggtagaa cggcacttcc gcgaatagct
     8881 tgtcgcgttg ctcggcggcg taggggatca ctcgaatatc ggcgcgtcgg ctcagatcgg
     8941 ttagcaccgc attgggaatg ggcggatgaa actggacatc gatctggccg gcaatcagcg
     9001 cgtcggcgcc ttcggcgaat cccatgtgca ccggagtgaa gctgtcgaag gaaataccga
     9061 gcactttaaa aatcgtctga acatgctgtt ccatgccgct ccccttggcg ccgacagcta
     9121 cccttttgcc ggcgaaatcg gcgacggacg ctatcggtac gccggcgagg cgcacgaaac
     9181 acatgggccc ggcattggcc ggcgccacca tgcggatggc gatattttta gtaaatggcg
     9241 ccgtggcatc tttagcgcgg ccgatccagt tggacgccat gaaagcaaac tcgagagtgc
     9301 cggcgtcgag ctgattggca ttgtgaatac tcgcgccgct agtctcgacg acacaccgat
     9361 cgccgactcc acgtccacgg ttaaaaatct ccgcgatcgc ctggccctgg gtgtaaaatg
     9421 tcccgccaag ctccgacgtt cccattcgta tcgccatcgc acctccgctc cttatccctg
     9481 tctctgtccc tcatacacca gcctggagcc gtgctgcaaa taaaccgcta aagatccggc
     9541 aaaatctgcc gaattaatgg ataatcgccg atttctcctg actgttgaaa cctaaccatg
     9601 ttcgtcgaaa aatggatgac cgccaatccg gacactgtgg ctccggagga gacgatttca
     9661 gcggtcgcgc ttgagatgag ccggcgcaag gtgcgccatt tcccggtgtg cgagccgagc
     9721 cgaaccggta ggcgaatggt cggcatcgtc gccaagttcg atatcgcgcg tggctttccc
     9781 aacgatctga atccgttttc cgtggaagtc accgaagact ctgtgccgaa accggtgtcc
     9841 agcgtgatga ccaagaaagt cattacggtg ttgcccgatt gcccgatcga agaagccgct
     9901 cgcttgctgc gcactcacaa aatcggctgc ctgcccgtgc tcaaagacaa ccagatcgtc
     9961 ggcatcatcg cccaatccga cctcttcgaa gctttcatcg acatgaccgc cgccaagagc
    10021 ggtggcacac gcatttttat cgagtcggat ttcgacgata gcccggtgcc gacggtgctc
    10081 gacttctgcc gtctcaaccg ggtcgaaatc cttagtctga tgtcattcca tgaaaaccgc
    10141 ctgcgccgcc gcgacatgtc gatattccgt ttcgccggca gactccccaa aggtttcatt
    10201 cccgccatgg ccgccgccgg ttatcgcgtc gtcagcgtcg gcgaatagcg atcaattttc
    10261 ctctctgtct ctgacggttt tcagttgctc gccgcaaagc aatagcgcta tagtcgcggg
    10321 cgaatttagc gactaggagc caccatgcca tttctcgatc gcgacggcag cgagatttat
    10381 tacgaaaccc acggtgacgg cccggcgttg cttttttgca gtgtcaccgg cctcgatcat
    10441 caagcctgga agttccacca ggtgccggag ttttcccgcg atcacaaagt catcctgttc
    10501 gactaccgcg gcaccggcaa gtcgagcaag a
//
LOCUS       DASQKS010000252         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3075711, whole genome shotgun sequence.
ACCESSION   DASQKS010000252 DASQKS010000000
VERSION     DASQKS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075711"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VE170_06555"
     CDS             <1..664
                     /locus_tag="VE170_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15133.1"
                     /translation="YRIVLFDFPHQGASEIVTGPGTVTLDEQVDILDTMVKATRGARL
                     PIICSASWGGVVALAYAARHPDRLKSLILASMGMKANQAMIELITNGLAMPSKDGSTV
                     GETVIKGVGQELPDAMKGRILGQFQRMSSEALEAFCQHGLTVISVRDLTEVVDLSKVR
                     CQTMLLHGENDVIIDAEDVRSLAAQIAHAEMRTIKNVGHFLHLEKEEILNVYDEVLAS
                     MR"
     gene            943..2229
                     /locus_tag="VE170_06560"
     CDS             943..2229
                     /locus_tag="VE170_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYJ15134.1"
                     /translation="MSKAFGLDFGTTNSALALAHGDRAVEVAHFGTGETTFRSILYVE
                     QDEASKSGKLRVVAGPDAIQGYLNAHAPGRLIQSTKSYLASRLFSRTQIFDQTFSLEE
                     LIGTLLRYLRKSAEGQFGDLGSKLVVGRPVHFSGTKDPKDDDFAVARLRKALGHAGFD
                     EVHFLPEPVAAAYKYRQRLDHDELVLIADFGGGTSDFSLVKLQPTQTSTAGLSDQVIG
                     NDGVGVAGDTFDSKLVRHLVAPMLGLGSQYKTEFGKVLPVPNWLYEHLERWHYLSFLK
                     TRKNMALLKQIRFKALAPEMLDALIELVDQDLGYRLYQAIEAVKCSLSEQTGSQFYFK
                     EAAIDIERSVGRGDFEHWIGAEVSAITACVDRLLNNCNIASQEIDVVFMTGGSSFVPA
                     IRGIFENKFPKASIRAGQEFTSVAEGLSLHALEQLE"
     gene            2293..3303
                     /locus_tag="VE170_06565"
     CDS             2293..3303
                     /locus_tag="VE170_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15135.1"
                     /translation="MQITLLGMMAQWKTVVLTLWLAIAWLGAPLHAQSAPDKITLGTI
                     TLSLNNLPIYVAQEKGFFVKENIFLEAVVLNASTLAIPVLIAGSTQVSASSAMTTIRA
                     VEKGAQLKIVGGLTNAPVYDLYASAKIKTFKDLKGSTIGVSGLITSDTVIMKEMLKAN
                     GLEYPRDYALRVMGSMPDRFLAMQTGNIAAAILSPPYSFGADEAGLNNLGATAKYTPN
                     FVQTVFNVRADFAQDKKALLVRFLRAILRADQWIHNQKEDTVRIIVKRLKYSEKHSEA
                     SWRYYINTNAIPKDGEINPKGLDKVMQLLVEDGTLIQPLPKTDKYIDMSYLAEARRTL
                     LP"
     gene            3339..5258
                     /locus_tag="VE170_06570"
     CDS             3339..5258
                     /locus_tag="VE170_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYJ15136.1"
                     /translation="MIRRSNDSPLVLRVIVGILLSLHFVLPAQTYAQAASKAIVMAWD
                     GTVPSFVKELIREGKLPNLAKLIAGGAVAEDVMAGNPSKTAPGFASLMTGAPPRVTGI
                     SGNRVPRAPRAQFTILDSLPGFDGAPLRAEPIWATAQRAGKKSVVSHIPAFAEERSND
                     VTRFAGYNLISGRDGIVTRRAVKSESSAPWNDPPASDAPPIEFDFTVGETQLIGLLID
                     DPADPQIGYDTAVIAASRDSQEIKTKLKPAPASPGGALFWSAPITVKGAGNVEARIYL
                     RLFDLKADGSDFFMYYTRPMRDLQLQQDAETSPTVRTFIGNGASILYQNGALGRNIAN
                     DGSGGAEARYLETMLFAQHQLMETNRWAIDNLPWDLYLAYTPFPDEAEHAWRGHLDTT
                     LPSYRPEIADKLRPFLEQVYRSSDDHLGQLLAKRGDDTVMAVISDHGVQGIHKRVALN
                     LWLQQHGFLALDGQNRVDLAKTKVLYPAVNNGYLLINSDDRKGGIVKSEERDELIDKL
                     REALFSLRDGQRQVVASIIDGATEGELYGLGGEVGGDIYIELAVGYDFDPRLSGGQLL
                     ADTEPYGTHGANPKQASMRTLMVFNGPGIQAGQKLANARIIDFAPTLAWLLGWSQPKD
                     ATGKVLYEAFTVNPR"
     gene            5319..>5523
                     /locus_tag="VE170_06575"
     CDS             5319..>5523
                     /locus_tag="VE170_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15137.1"
                     /translation="MAFNAAWEPPQVVSFDEVLKLNDDVTARAGTPIDISEDIFRIHE
                     LELDWDIGVVRYTPQDASRIPTGP"
BASE COUNT         1202 a         1666 c         1548 g         1107 t
ORIGIN      
        1 ctatcggatc gttctatttg attttccgca ccagggggca agcgagatag ttaccggacc
       61 cggcaccgtc accctggacg agcaggtcga catattggat accatggtaa aagccacgag
      121 gggggcacgc ctgccgatta tttgttcggc gtcgtggggc ggggtggtgg cgctggccta
      181 cgcggcgcgc catccggata gactgaaaag tttgatcttg gccagcatgg ggatgaaggc
      241 gaaccaagcg atgatcgagt tgatcaccaa cggattggcc atgccgagca aagacggatc
      301 aacggtgggc gagacggtga tcaagggtgt cggtcaggaa ctgccggacg ctatgaaggg
      361 ccgtatactc ggccagtttc agcgcatgag cagcgaggcg ctggaggctt tttgtcagca
      421 cggcttaacg gtcatatcgg tccgcgactt gaccgaggtc gtcgatctta gcaaagtccg
      481 ctgccagacc atgctgctgc atggcgagaa cgacgtcatt atcgacgccg aagatgttcg
      541 gtcgttggcc gcgcagattg cccacgcgga gatgcgcacg atcaagaacg tgggacattt
      601 tctccatctt gaaaaggaag agatcttgaa tgtctatgac gaagtcctcg cctcaatgcg
      661 ctgattaaag gcgccggtct gcctatattg ctgtgctgtg gcattccggc ctttgctcta
      721 taccaaagtt atctaaaaga aataggtcgc ctcatagtgg cacgttctta ttttactcat
      781 ccagacgcta ggtattaggc tttcccaggc aaccgatctt gtttatgcat cccatgcgcc
      841 ctccggcgca acctgacgcc tgacttttta ccgctcgctg ttcaggaatt cccggcgaaa
      901 tgttacggtg tcgggattcg catcaccgcc acggagattg ttttgtccaa agcgttcggt
      961 ctcgacttcg gcacgaccaa tagcgcgctg gctctcgccc acggcgaccg cgcggttgag
     1021 gtcgcgcatt ttggcaccgg cgagacgacc tttcggtcga ttctttacgt tgagcaagac
     1081 gaggcatcga aaagcggcaa gctgcgcgtg gtcgccgggc ctgacgcgat ccaaggctat
     1141 ttgaatgcgc atgcgccggg acggttgatc cagtcaacca agtcctatct cgccagccgc
     1201 ttatttagcc ggacgcagat attcgatcaa actttctccc tggaagaact tatcggcacc
     1261 ttgctgcgct atctgcggaa atccgccgaa ggccaatttg gcgatctcgg cagcaagctg
     1321 gtggtcggcc gcccggttca tttcagtggc acgaaagatc caaaagacga cgacttcgcg
     1381 gttgcgcggt tgcgcaaagc ccttggtcac gccggcttcg acgaggtcca cttcctgccc
     1441 gagcccgtcg ccgccgccta caaataccgc cagcggctcg accacgacga gctcgtgctg
     1501 atcgccgact ttggcggcgg caccagcgat ttttctctgg tcaaattgca accgacgcaa
     1561 acttcgacag ctggattgag cgatcaagtg atcggcaatg acggcgtcgg cgtcgccggc
     1621 gatacttttg acagtaaact tgtgcgccat ctggtggcac cgatgttggg cttgggcagc
     1681 caatacaaga ctgagttcgg caaagtgctc cccgtgccca attggcttta cgagcacttg
     1741 gaacggtggc attacctgtc gtttctcaag actcggaaaa acatggcgct gctcaagcaa
     1801 attcgcttca aagccctggc gccggaaatg ctcgacgcgc tgatcgagct ggtcgaccag
     1861 gatctcggct accgcttgta ccaggcgatc gaggcggtca aatgcagcct atcggaacag
     1921 acgggcagtc aattttattt caaagaagcg gccatcgata tcgagagatc cgtcggacgc
     1981 ggcgatttcg agcactggat cggcgccgaa gtgagcgcta ttaccgcctg cgtcgatcgc
     2041 ctgctcaaca attgcaacat cgcttcacaa gagatcgacg tggtgttcat gaccggcggt
     2101 tcgtcgttcg tgccggcgat ccgcgggatt tttgaaaaca aatttcccaa ggcgtcaatc
     2161 cgcgccggcc aggagttcac ctcggtcgcc gaggggttat cgttgcacgc actcgaacaa
     2221 ctcgaatgaa ccgatctgcc agggcgcagc cgcgttgaca ctgcgaatcg caatcggcta
     2281 ctgtcggggc aaatgcaaat cacacttcta ggcatgatgg cgcagtggaa aacagtcgtc
     2341 ttaacgctct ggctggcaat cgcctggcta ggcgcgccgc tgcatgccca gagcgcgccg
     2401 gacaagatca ctctcgggac gatcacgctc tcgctcaaca atttgccgat ttacgtcgcc
     2461 caggaaaaag gctttttcgt caaagagaat atttttctcg aagccgtggt gttgaacgcc
     2521 agcacgttgg cgatcccggt gctgatcgcc ggatcgaccc aggtgtcggc gtcctcggcc
     2581 atgaccacca tccgggcggt ggaaaaaggc gcacagttaa aaatcgtcgg cggcctgacc
     2641 aacgcgccgg tttacgatct ctatgccagt gccaaaataa aaactttcaa ggatctcaaa
     2701 ggcagcacca tcggcgtctc cggcttgatc accagcgaca cggtgatcat gaaagagatg
     2761 ctcaaggcca acggcctgga gtatccgcgc gattacgcct tgcgcgtcat gggcagcatg
     2821 cccgaccgtt ttttggcgat gcagaccggc aacatcgccg cagcgatctt gagcccgcca
     2881 tacagcttcg gcgccgacga agccggcttg aacaatctcg gcgccaccgc caagtacacg
     2941 ccgaatttcg ttcagacggt gttcaacgtg cgcgccgatt tcgcccaaga caagaaggcg
     3001 ctgctggtgc ggtttctgcg cgcgattttg cgcgccgacc agtggatcca caaccagaag
     3061 gaagacaccg tgcgcatcat cgtcaagcgc ctcaagtata gtgaaaagca ttcggaagcg
     3121 agctggcgct actacatcaa caccaacgcc attcctaaag acggcgagat caatcccaag
     3181 gggctggaca aggtcatgca attgctcgtc gaagatggca ctctgatcca gccgttgccc
     3241 aagaccgata agtatatcga catgagctat ctcgccgaag cgcgccggac gctactacca
     3301 taaattttta cacaacttgg gtttaccaca tcgcgcttat gatccgccga tccaacgatt
     3361 cgccgctggt gcttcgcgtc atcgttggca ttctgctgtc gctccacttt gtcctgccgg
     3421 cgcagactta cgcgcaggca gcgtcaaaag ccatcgtcat ggcttgggac ggcaccgtgc
     3481 cctcgttcgt taaggaactc attcgcgaag gcaaactgcc caacctcgcc aagctcatag
     3541 ccggcggtgc cgtcgccgaa gatgtcatgg ccggaaatcc ctcgaagacg gcgccgggat
     3601 ttgcctcgct catgaccggc gcaccgccgc gcgttaccgg catcagcggc aaccgcgtgc
     3661 cgcgcgcgcc ccgcgcacag ttcacgattc tcgacagcct ccccggcttc gacggcgcgc
     3721 cgctgcgcgc cgagccgatc tgggccacgg cgcaacgcgc cggcaagaag tcggtggtat
     3781 cgcatattcc ggcgtttgcc gaagaacgat ccaacgacgt aacacgcttc gccggctaca
     3841 atctcatctc aggccgcgac ggcatcgtca cccggcgcgc cgtcaagagc gagagcagcg
     3901 cgccctggaa tgatccgccg gcgagcgatg cgccgccgat tgaatttgat ttcactgtcg
     3961 gcgaaaccca gctcatcggc ctgctgatcg acgatcccgc cgatccgcaa atcggctacg
     4021 acactgctgt gatcgcggcc agccgcgata gccaggagat caagactaaa ctgaagccgg
     4081 cgccggccag tccaggcggt gcactcttct ggagcgctcc catcactgtc aagggcgcgg
     4141 gcaatgtcga ggcgagaatc tatttgcgtc tattcgatct caaagcggac ggcagcgatt
     4201 ttttcatgta ctacacccgg cccatgcgcg atctccaatt gcagcaagac gccgaaacca
     4261 gccccaccgt gcgcaccttc atcggcaatg gcgcgagcat actctatcaa aatggcgccc
     4321 tgggccggaa catcgccaac gacggcagcg gcggcgccga agcgcgctat ttggagacca
     4381 tgctgtttgc ccagcaccag ttgatggaaa ccaaccgctg ggccatcgac aatctgccct
     4441 gggatctata tctcgcttac acgccgttcc ccgacgaggc cgagcatgcc tggcgcggcc
     4501 acttggacac cacactgccc agctatcgtc cggagatcgc cgacaagctt cggccgtttc
     4561 tcgaacaagt ttatcgttcg agcgatgacc atctcggaca attacttgcc aagcggggcg
     4621 acgatacggt gatggcggtg atatccgacc acggcgtcca aggcatccac aagcgggtcg
     4681 cgctcaacct ttggttgcaa caacatggct ttctcgctct tgacgggcag aaccgcgtcg
     4741 atctggcaaa aactaaagtt ctctacccgg cggtcaacaa cggctacttg ctgatcaatt
     4801 cagacgaccg caaaggcggc atcgtcaaga gcgaagaacg ggacgagttg attgacaagc
     4861 tgcgggaagc gctcttttcc ctgcgcgacg gccaacgcca agtagtcgcc tcgatcatcg
     4921 atggcgcaac cgaaggagaa ctgtatggcc taggcggtga agttggcggc gacatctata
     4981 tcgagctcgc tgtcggctac gacttcgatc cgcgcttgag cggcggacaa ttgcttgccg
     5041 acaccgagcc ctacggcacc cacggcgcca atccgaagca agcctccatg cgcacactca
     5101 tggtattcaa cggccccggt attcaggcgg ggcaaaaatt agccaacgcg cggatcatcg
     5161 atttcgcccc aacccttgcc tggctgctcg gctggtccca acccaaagat gccaccggca
     5221 aagtgcttta cgaagctttt accgttaacc cgcgttgaca tcggcggcgt cgtctcataa
     5281 tttccgtcat agcaaatttg tccttgggag aattttccat ggcgttcaat gccgcttggg
     5341 aaccgccgca ggtggtctcc ttcgacgagg ttctcaaact caacgacgac gtcacggcca
     5401 gagccgggac ccccatcgac atcagtgaag atatctttcg catccatgaa ctggaactgg
     5461 attgggacat cggcgtggtg cgctacacgc cgcaggacgc aagccgcatc cccaccggac
     5521 ccg
//
LOCUS       DASQKS010000253         9332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6026998, whole genome shotgun sequence.
ACCESSION   DASQKS010000253 DASQKS010000000
VERSION     DASQKS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6026998"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..762)
                     /locus_tag="VE170_06580"
     CDS             complement(160..762)
                     /locus_tag="VE170_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ15138.1"
                     /translation="MMQRFEYLKTVAADAQDALVVSTGWGSREWGAVRPSEGNLKTRT
                     LGLVSSIAAGLAIALPHRKVIAIDGDGAFLMNLCGLPTIARQSPGNLIHLLFDNEIYE
                     ASGGTATASRSTDAVAIAKGAGYPHALWVDSPEAFRGAFLRAWEGSELTLIAVKVDPG
                     QPKNLPPLKLDEIENKYRFMRYLEASENKGILNPGFDSKL"
     gene            complement(759..1268)
                     /locus_tag="VE170_06585"
     CDS             complement(759..1268)
                     /locus_tag="VE170_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ15139.1"
                     /translation="MTTNPAAAGAAIVAGLKKAGIDFVATLPDEKMLEVIRAVERDRQ
                     LKHVPLCREEEGIGICAGAYLAGKKTAIIMQNAGFLNSCNALTTTSLQLQIPILMLIY
                     YAGDIGDRGFTTLGAVTEPVLQAMGFRTYVLRKPEDIDEVLAGAQILADDSKKPVAVL
                     LTKSVLGVR"
     gene            1414..1800
                     /gene="gcvH"
                     /locus_tag="VE170_06590"
     CDS             1414..1800
                     /gene="gcvH"
                     /locus_tag="VE170_06590"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HYJ15140.1"
                     /translation="MMEKEVKVSKQHVWVGVEDRHVHLGLTNYIQGALGSVLSVELPD
                     VGDSIEQGEIFAEIESASTVHELVSPVTGTVMAVNSALEDHPAVINEDPHSEGWLIEV
                     RLKNEEELDSLMDMDEYYHFVFKDKS"
     gene            1763..2536
                     /locus_tag="VE170_06595"
     CDS             1763..2536
                     /locus_tag="VE170_06595"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYJ15141.1"
                     /translation="MSITISFSKINPKPALFSPAFILGFGVVWLFAALAVLGGAEPED
                     RYAVPRRIMIERDLRARGITDSRVLAAMAVTPRHLFVPEAVRGSAYDDRPLAIGDGQT
                     ISQPYIVALMSELLEIQDQDKVLEIGTGSGYQTGILAQLAKEVYSIEIIMWLSERAKP
                     ILDRLGFRNVFLKIGDGFFGWEERAPFDAILVTAAAPKVPEALWRQLREGGRIVMPLG
                     DPGQIQRLVRIHKRAGKEFVETLTDVRFVPLTGAVQEPR"
     gene            complement(2484..2849)
                     /locus_tag="VE170_06600"
     CDS             complement(2484..2849)
                     /locus_tag="VE170_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HYJ15142.1"
                     /translation="MMLQLSTRWPFYLLGSLILLLSVVTIVGERGALHLWRLGGEKSR
                     IDEQNYRMQRDNEMLRQKVSRVRHDNHYLEQLAREELNMARPGEVIYRFSKAESRRIP
                     SAVNEAPEPLPSKERSGRR"
     gene            2918..4204
                     /gene="eno"
                     /locus_tag="VE170_06605"
     CDS             2918..4204
                     /gene="eno"
                     /locus_tag="VE170_06605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYJ15143.1"
                     /translation="MKIQRVHAREVLDSRGQPTVEVEVTLKNGVLGRATVPSGASTGV
                     HEAMELRDGGKRFLGKGVARAVAHVNERIAPRLRGKDGRDQVAVDRIMLDLDGSANKS
                     KLGANAILGVSLAVAQAQALSQGLSLYRYLGGAAGRTLPVPMLNVLNGGAHADNNVDV
                     QEFMVVPYGMKSFKEALRGAAEIFATLKMVLHDRGLSTSVGDEGGFAPRVKSNAEAIE
                     LLLEAVAKAGYKTGSQVALALDVASSEFYDDGKYVFKKSDGQSRSREQLVQLYQDWVR
                     QYPIISIEDGFAEDDWEGWRMMTQALGKKIQLVGDDLFVTNKVRLKRGIDSGVANSIL
                     IKVNQIGSLTETLETMQLAKENGYTTVMSHRSGETEDVTIADLAVATNAGQIKTGAPC
                     RGERTAKYNQLLRIEEELGKRAVYAGKSAFRVRRAG"
     gene            4164..4904
                     /gene="nth"
                     /locus_tag="VE170_06610"
     CDS             4164..4904
                     /gene="nth"
                     /locus_tag="VE170_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013170889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYJ15144.1"
                     /translation="MPARAPFGFDAPAKALGNAAPASAKARIRGAESAQEKAERAAAI
                     AAGLAGLYGAVECPLTHRNNFELLVAVILSAQCTDAAVNKVTPELFARYPNPLALSQA
                     SLADIESVIRTLGLFRAKAKSLKRCAEQLVLNHHGEVPSAMADLIELAGVGRKTANVI
                     RGHAFGEPGIAVDTHCRRLARRLRLTRQQNPVKIEIDLARLLPSSEWTNFSHRLIIHG
                     RRVCFARKPACDRCSLRRLCPSAQSQPG"
     gene            complement(4977..5834)
                     /locus_tag="VE170_06615"
     CDS             complement(4977..5834)
                     /locus_tag="VE170_06615"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HYJ15145.1"
                     /translation="MSARFFGQYLLEKGRINSQQLLTALELQKTVLRPIGIMALERGW
                     LGAARITEILELQKRKSLRFGELAMASGYLTQLQVDELLQVQETSHRVRLGEALVTKG
                     YMTMESLERELKEYNKESEKFTSDVSSAFNALPHKQIVKTFTDLMLVMFTRFGKQDII
                     IERCESGKEKLRLFRWVIAQKITGANIAFNCLLSVPPKLLLQMASTMLDDNIDIADEL
                     TLDASKEFVNIANGNACAKLSEDGVELTMLPPEVYETTAKPYPFQTNEVICVHLASPD
                     AKLDVAFEF"
     gene            complement(5831..6214)
                     /locus_tag="VE170_06620"
     CDS             complement(5831..6214)
                     /locus_tag="VE170_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ15146.1"
                     /translation="MNKVKVLAVDDSKTMLAMLSAQLRDSNFEVIATASSGLEAVEKF
                     KELKPQLVLLDVVMPELTGIDTLERLLNVDTTACVVMVSSIGTEATVKDCLQRGAKSY
                     IQKPLQKDNVLAHLKKVCQEAGVTL"
     gene            complement(6293..7423)
                     /locus_tag="VE170_06625"
     CDS             complement(6293..7423)
                     /locus_tag="VE170_06625"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HYJ15147.1"
                     /translation="MSPGKRSPLNILVVDDSAVVREVMTAVLAQEPGFSVTVAGDPFI
                     AMGKMKQKRPDVIVLDLEMPRMDGLTFLRKIVREDFIPVVICSALTGPDTRIGLQALE
                     EGAVDVVTKPRLGVRDFLHESAVVLIDAVRAAARCAARKPIAAPLAATPRLKADVMLP
                     GKSAMPVRITSDKVVAIGASTGGTEALKSILEAMPPDAPGMVVVQHMPEGFTAAFAQR
                     LNQACRIEVKEAADGDRVFAGRVLIAPGNLHILVRRNGAQYSVEVADGPLVSRHRPSV
                     DVLFRSVAQSAGGNAVGVIMTGMGNDGAEGLLEMQQAGAFTLAQDESSCVVFGMPKEA
                     IERGAVDRVVNLAGITAAILSGRTTDKGQLTRPALNAAHQGK"
     gene            complement(7423..8355)
                     /locus_tag="VE170_06630"
     CDS             complement(7423..8355)
                     /locus_tag="VE170_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HYJ15148.1"
                     /translation="MNQTMPVPAQASTDAVIRPVSDHEFALFQKLIYSKAGIHLAPAK
                     KTLLEARLTRRLRELGLHSFAAYYQKVLAVDDSEELIILLNRIATNETHFFREPKQFE
                     FLDDHIIPAWQSQGHGANGTRTIRVWSAACSTGEEPYSIAMLLSERFAPGSGWDFEIL
                     ATDLSTRVLAAAGAAVWPIAKASEIPDEYLKKYMLKGIGEQNGKMKAGAELRSTIRFE
                     RLNLNEAHYPIDGNFDLIFCRNVLIYFDTQSRARAITRLIDHLAPNGYLFVGHAESLH
                     NVTDRLRYIVPTIYSNDRQPRTAINQKRDGVESI"
     gene            complement(8537..>9332)
                     /locus_tag="VE170_06635"
     CDS             complement(8537..>9332)
                     /locus_tag="VE170_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ15149.1"
                     /translation="AAAVEETTSSLQQMNASISQNADISRQMEQMALTGVRNVEKSGT
                     AVMESVDAMQTIAEKIMIIEEIAYQTNLLALNAAIEAARAGEQGRGFAVVATEVRKLA
                     ERSQVAAQEIGSLTISSLKVAEHSGALLKDLVPAIRKTAELVQEVTTASREQSTGVNQ
                     VNRAMIQVDQVTQNNAAAAEELSGTAGQLTAQAQELQKLVSSFRIHAEVMGTARAAWP
                     AAARSNDARLHMENTTPARRGASSVAGVKGGLRQLNQDQDRDYQRF"
BASE COUNT         1895 a         2614 c         2708 g         2115 t
ORIGIN      
        1 gccgctatcc cgatcagtgg aactggctcg gcctacccaa tcgtaatggt aagttgtcgc
       61 gcgccgaact ggcactgcgc aggaaagaac ggattaaaga agccgcctcg cgagaagtcg
      121 ctgtaacgcg aaaaaccggt accggcgcgt agccgccaac tataatttcg agtcgaatcc
      181 cggattcaga atgcctttgt tctcgctcgc ctccaagtag cgcatgaagc ggtatttgtt
      241 ctcgatctcg tctagtttta gcggcggtaa atttttcggc tggccgggat cgactttcac
      301 ggcgatcaag gtcagctcgc taccctccca agcgcgcagg aatgcaccgc gaaacgcctc
      361 cggtgagtcg acccacaggg cgtgagggta acccgcgccc ttggctatgg ccaccgcgtc
      421 agtactgcga gaagcagtgg cggtgccgcc ggacgcttca tagatttcgt tgtcgaaaag
      481 caggtggatc aaattgcccg ggctctgccg agcgatcgtc ggtaaaccgc ataggttcat
      541 cagaaaggcg ccatcgccat cgatggcgat cactttgcgg tgcggtagcg cgatggctag
      601 gccggcggcg atggaagaca ccaagccaag ggtgcgggtc ttgagattgc cttcgctcgg
      661 ccgcaccgcc ccccattcgc gcgagcccca accggtggac accaccaagg catcttgggc
      721 gtccgcggca acagtcttca gatattcaaa gcgttgcatc atcttactcc caggacgctc
      781 ttggtcagca gcaccgccac cggttttttc gaatcgtcgg cgagaatctg cgcgccggcc
      841 agcacttcat cgatgtcctc tggcttgcgc aatacgtagg tgcggaatcc catcgcctgc
      901 aagaccggtt cggttaccgc gccgagggtg gtaaatccgc ggtcgccgat atcgccggcg
      961 tagtagatca gcatcaagat cggtatttgc aactgcagcg atgtggtggt cagcgcgttg
     1021 cagctattca aaaagccggc gttctgcata atgatcgcgg tcttcttgcc ggcgagatag
     1081 gcgccggcgc aaatgccaat cccttcttcc tcgcggcaca gcggtacatg cttcaattgt
     1141 cggtcgcgtt cgacggcacg gatcacttcg agcattttct cgtccggcag ggtggcgaca
     1201 aagtcgatgc cggccttttt caagccggcg acgatggccg cgccggcagc cgcgggattc
     1261 gttgtcatat tgctcctatg ggtgagaacg ccgcatgatt tgctttcccg attcgactat
     1321 cgctactgtg aattccatga tgatgtcaag gcggagcggc aacacttctc ttgacaggat
     1381 cacgacaaag tttgtaggga tggattacca accatgatgg aaaaagaagt caaagtcagc
     1441 aaacagcatg tctgggtcgg tgtcgaagac cgccatgttc atctcgggct caccaactat
     1501 attcagggtg cactcggttc ggttctttcg gtcgagctgc cggatgtcgg cgacagcatc
     1561 gaacagggcg agatctttgc cgagatcgaa tcagcctcga cggtgcatga gctggtctcg
     1621 ccggtgaccg gaacagtgat ggcggtcaat tctgcgttag aggatcatcc cgcggtcatc
     1681 aacgaagatc cccacagcga aggttggctc atcgaagtgc gcttaaagaa tgaggaggaa
     1741 ctcgactccc tcatggacat ggatgagtat taccatttcg ttttcaaaga taaatcctaa
     1801 accagcgctt tttagcccgg cgtttatcct cgggttcggt gtcgtgtggc ttttcgccgc
     1861 gctggcagtt ttgggtggcg cggaaccgga agaccgttat gccgtgccgc ggcgcatcat
     1921 gatcgagcgc gatctacggg cccgcggcat cacggactca agagtcctgg cggcgatggc
     1981 ggtgacgcca cgccatctgt ttgtacccga ggccgtgcgc ggctcggcct acgatgaccg
     2041 gccgctggcc atcggcgacg gccagacgat ttcgcagccc tacatcgtcg ctttgatgtc
     2101 cgaactgctc gaaattcaag accaggacaa ggtccttgag attggcaccg gttccggcta
     2161 tcagaccggg atcttggctc agctcgccaa ggaggtctat tcgatagaaa tcatcatgtg
     2221 gctgagcgag cgcgccaaac cgatattgga ccggctcggt tttcggaatg tttttctgaa
     2281 aatcggcgac ggatttttcg gctgggaaga aagggcgcct ttcgacgcca ttctggtaac
     2341 ggcggcggca cccaaagtgc ccgaagcgct atggcggcaa ctacgggaag gcgggcgaat
     2401 cgtgatgccc ctgggagacc cgggacaaat tcagcgcttg gtgcggatac acaaacgcgc
     2461 cggcaaggag ttcgtcgaga cgcttaccga cgtccgcttc gttcctttga cgggagcggt
     2521 tcaggagcct cgttgacggc gctggggatg cggcgcgact cggcttttga aaagcggtag
     2581 atcacttccc ccgggcgcgc catgttgagt tcctcgcgcg ccaattgttc cagataatga
     2641 ttgtcgtgac ggacgcggga aaccttttgc cgcagcattt cgttgtcgcg ctgcatgcgg
     2701 tagttttgtt catcgatgcg gctcttctcg ccgcccagac gccagaggtg cagcgcgccg
     2761 cgctcaccga cgatggtgac gacggagagc aatagaatca gcgaaccgag caagtagaac
     2821 ggccagcgtg tcgacaactg aagcatcatc gggattaatg gcaatatagc atattttgtg
     2881 attttgtctt gtctatcaat ttttccagga gacgacgatg aaaattcaac gagtgcatgc
     2941 gcgggaagtg ctcgattcgc gcggccagcc gacggtggaa gtcgaagtga cgctcaaaaa
     3001 cggcgtgctc ggaagggcga ctgtgccttc cggcgcgtcc accggcgtcc atgaggcaat
     3061 ggagttgcgc gatggcggca agcgttttct cggcaaagga gtcgcccgcg cggtcgccca
     3121 tgtcaatgaa agaatcgcgc cgcggctgag aggcaaggac ggccgcgacc aggtcgccgt
     3181 cgaccgcatc atgctcgacc tcgacggctc tgcaaacaaa agcaagctcg gtgccaacgc
     3241 aattcttgga gtctccttag cggtggctca ggcccaggcg ctttcccaag gtttgtcgct
     3301 ctatcggtat ctcggcggcg cggcaggcag gactctgccg gtgccgatgt tgaacgtcct
     3361 caacggcggc gcccatgccg acaacaatgt tgacgtgcaa gaattcatgg tggtccccta
     3421 cggcatgaaa agttttaaag aagcgctgcg tggcgccgcg gaaatcttcg cgactttgaa
     3481 aatggttctt catgaccgcg gtctgtccac cagcgtcggt gatgaaggcg gttttgcgcc
     3541 gcgggtcaag agtaacgccg aggccatcga gctcttgctc gaagctgtcg ctaaagccgg
     3601 ttacaaaact ggctcccaag tagccctcgc gttggatgtc gcctccagtg aattttatga
     3661 cgacggcaag tatgttttta agaaatccga cggccaaagc cgcagccggg agcaattggt
     3721 gcagttgtac caagactggg tgcgccagta tccgattatt tccatcgaag atggttttgc
     3781 cgaggacgac tgggaaggct ggcgcatgat gacccaggct ttgggcaaga agattcagct
     3841 ggttggcgac gatttgttcg tcaccaataa agtgcgtttg aagcgcggca tcgactcggg
     3901 cgtcgccaac tcgatcttga tcaaggtcaa tcagatcggc agtctgacgg aaactttgga
     3961 gaccatgcag ctggccaaag aaaatgggta cactacggta atgtcacacc gttccggtga
     4021 aaccgaggat gtcaccatcg ccgatctggc ggtggcgacc aacgccggcc aaatcaaaac
     4081 cggcgcgccc tgccgcggtg agcgcacggc caaatataat cagctcctgc gtatcgaaga
     4141 ggaactagga aagcgagccg tctatgccgg caagagcgcc tttcgggttc gacgcgccgg
     4201 ctaaagcgct gggcaatgca gctccggcga gcgctaaagc acggattcgc ggggcagaat
     4261 ccgcgcagga aaaagccgag cgtgccgctg ccatcgctgc cggcttggcg ggtctctacg
     4321 gcgcggtcga gtgccctctc acacaccgta acaatttcga gctgctggta gcggtgattc
     4381 tgtccgccca gtgcacggat gcagcggtaa acaaggttac gccggaacta ttcgcgcgct
     4441 atcccaaccc cctcgcgctc tcgcaagctt cgttagccga tatcgagtca gttattcgca
     4501 ccttgggatt atttcgcgcc aaagccaaat cgctcaagcg ctgcgccgag cagttggtcc
     4561 taaatcacca cggcgaagta ccttcagcga tggcagatct catcgagctt gccggtgtcg
     4621 gtagaaagac cgccaacgtc attcgcggcc atgcctttgg tgagccgggg atcgccgtag
     4681 atacccactg ccgccgcctg gcacgccgtt tacggctaac ccggcagcag aacccggtga
     4741 agatcgaaat cgacttggcg cgtctcctgc catcttcgga gtggactaat ttctcgcatc
     4801 gattgattat ccatggacgg cgcgtatgtt ttgcccgcaa gccggcctgt gatcgttgct
     4861 cgttaaggcg cttgtgcccg tcggcgcagt cccaacccgg ctgagctgcc cagccgatgg
     4921 gcaactcaag ccgactccaa cccggcgaat ttattgatgg cagacaaggc tatgcgctag
     4981 aactcgaagg ccacgtcaag ctttgcatcc ggcgacgcga gatgaacgca gatcacttcg
     5041 ttggtctgaa acggataggg cttggcagtg gtctcgtaga cttccggtgg cagcatggtg
     5101 agctcgacac cgtcctcgga cagtttggcg caggcgtttc cgttggcgat gttaacgaac
     5161 tctttcgacg catctaaggt taattcatcg gcgatgtcga tgttgtcgtc caacatggtc
     5221 gaggccatct gcaatagcaa cttggggggc accgacaaca ggcagttaaa ggcgatgttg
     5281 gcgccggtga tcttttgggc gatgacccaa cggaacagcc ggagtttctc tttgccgctt
     5341 tcgcagcgtt cgatgataat gtcttgctta ccgaatcggg taaacatcac cagcatcagg
     5401 tcggtaaacg tcttgacgat ttgtttgtgc ggcagggcgt tgaaggcact gctgacgtcg
     5461 gaagtaaatt tttcgctttc tttattatat tcctttagtt cacgctcgag cgactccatg
     5521 gtcatatacc ctttggtcac cagcgcttcg cccaatctta cccggtggct ggtttcctga
     5581 acttgcagca attcatcgac ctggagctga gtaaggtagc ccgaggccat cgccaattcg
     5641 ccaaaacgca aagactttcg cttctgcagt tcgagaatct ccgtaatccg ggctgcgccg
     5701 agccagcctc gttccagcgc catgattccg atgggcctca agacagtttt ttgcaactcc
     5761 agagcggtga gcagctgttg cgagttgatc cgacctttct cgagcaaata ttggccaaaa
     5821 aatctcgcgc tcatagtgtc accccggctt cctggcacac tttctttaaa tgcgccagca
     5881 cattgtcctt ctgcagcggt ttttgaatgt aactctttgc gcctcgctgg aggcaatcct
     5941 tgacggtcgc ctcggtaccg atagagctga ccatcaccac gcaggcggtc gtgtcgacat
     6001 tgagcagccg ttcgagggtg tcgatgccgg tcagctcggg catgacgaca tcgagcagca
     6061 ctagttgcgg tttgagctct ttgaattttt ccacggcctc gaggccgctg ctagcggtgg
     6121 cgatgacttc aaaattgcta tcgcgtaact gtgccgagag catggcgagc atggttttgg
     6181 agtcatctac ggcgaggact tttactttgt tcacgagttt tattctcctt ccgaaattga
     6241 cttggttttg agttggttgt ctggttaagt gctgagccta tgtttctggt gctcattttc
     6301 cctggtgtgc ggcgtttaac gccggccggg tcagctgccc tttatcggtg gtccgtccgc
     6361 tcaagatcgc cgccgtgatt ccggcaaggt ttaccacccg gtccaccgcg ccgcgctcga
     6421 tcgcttcctt gggcatgcca aaaactacgc acgaggattc gtcctgggcc aaggtgaacg
     6481 cccccgcctg ttgcatttcc aagagtcctt cagcgccgtc attgcccatg ccggtcatga
     6541 ttacgccgac ggcattgccc ccggcacttt gcgccacgga acgaaatagg acgtcaacgc
     6601 tcggacggtg gcgtgacacc aaaggaccat cggcgacttc gacggaatat tgagcgccgt
     6661 tgcggcgaac taggatgtgg agattccccg gcgcgatcaa gacgcgtccg gcgaagacgc
     6721 ggtcgccgtc agcagcctcc ttgacctcaa tgcgacaagc ttggtttaag cgttgggcaa
     6781 acgcggcggt gaacccttcc ggcatatgtt gcacgaccac cattccgggg gcgtccggcg
     6841 gcatggcttc aaggatcgac tttagtgctt cggtaccacc ggtagaagcg ccgatggcaa
     6901 cgactttgtc gctggtgatg cgtacgggca tagccgattt gccggggagc atgacgtcgg
     6961 ctttgagccg cggcgttgcg gccaatggcg ctgcgatggg ttttcgcgcg gcgcaccgcg
     7021 ccgccgcccg cacggcatcg attaatacca ccgccgattc gtggagaaag tcacggacgc
     7081 ctaagcgcgg tttggtgacg acgtcgacgg cgccctcctc aagcgcttgc agcccgatac
     7141 gggtgtcggg tccggtcaat gccgaacaaa tgaccacggg aataaagtct tcgcggacga
     7201 tcttgcgcaa aaatgtcagg ccgtccatgc gcggcatctc gagatcgagc actatgacgt
     7261 ccggccgttt ttgcttcatc ttacccatgg cgataaatgg atcgccggca acggtcacgc
     7321 tgaagccagg ctcttgcgct aagaccgcag tcatcacctc gcggactaca gcggaatcgt
     7381 cgacgaccaa gatatttagt ggactgcgtt tgccgggact cattaaattg attcgactcc
     7441 atcacgtttt tgatttatgg ccgttcgcgg ttgcctgtcg ttgctgtaaa tcgtcggtac
     7501 gatatagcgc agccggtcgg tgacattatg caaactttcg gcatggccga cgaataaata
     7561 gccgttgggc gcgaggtgat cgatcaggcg cgtgatcgct cgagcgcggg actgggtgtc
     7621 gaaatagatc aaaacattgc gacaaaagat caggtcgaaa ttgccatcga tcgggtaatg
     7681 cgcctcgttc aagttgagcc gctcaaagcg aatcgtcgat cgtaactcag ccccggcttt
     7741 catcttgcca ttctgctcgc cgataccctt aagcatatat tttttaagat actcatcggg
     7801 aatctcgctg gccttggcga tgggccagac tgccgcgccg gcagcggcga gtacgcgagt
     7861 cgataaatct gtcgccaata tttcaaaatc ccaacccgag ccgggcgcaa agcgctccga
     7921 cagcagcatg gctatagaat agggctcttc gccggtcgaa caagccgcgc tccagacccg
     7981 gatggtgcgc gtgccgttgg cgccatgccc ctgagactgc cacgcgggaa tgatatgatc
     8041 atccaggaac tcgaactgct tgggctcgcg aaaaaaatgg gtttcgttgg tcgctatgcg
     8101 gttgagcaag atgatcagct cttcgctgtc gtccacggcg aggacttttt gataataggc
     8161 ggcaaacgaa tgcaggccca attcgcgcaa gcgtcgtgtc agacgcgcct ccagcaaggt
     8221 tttttttgcc ggcgcgagat ggatccccgc cttgctgtag atcaactttt ggaacagcgc
     8281 gaactcatga tcgctcaccg ggcggataac cgcgtcggtg ctcgcttgag ctggcaccgg
     8341 catagtctgg ttcatggtca tggtgtttaa tcggcactca tgccgttgta gcgtctgtcg
     8401 tcggcgactc tggacacttc atcaccggtt tgtcgctgtc gttagcggct ggcgagatcg
     8461 cggcggatct taggggtgtc tctaagttct tcattcttcg tcaaccttta ttgccttcgt
     8521 tacggtgaca gattctttag aatcgttgat aatctcgatc ctggtcttga tttagctgtc
     8581 gcaatccgcc tttgaccccg gcaacgctcg aggctccacg ccgcgccgga gttgtgtttt
     8641 ccatatgtaa cctggcgtca tttgaccggg ccgcggcagg ccatgccgct ctcgccgtcc
     8701 ccattacctc ggcgtggatc cggaacgaac tcactagctt ctgtagttct tgcgcctgag
     8761 ctgtgagttg tccggctgta ccggatagtt cctcggcagc ggcggcattg ttttgggtca
     8821 cttgatcgac ttggatcatc gcacggttga cttggttcac accggtgctt tgctcacgcg
     8881 aggctgtcgt cacttcttga actaactcgg cggttttgcg aatagccgga acaagatctt
     8941 tcaacagagc accggaatgt tccgcgactt taagactgga tatcgtcagc gaaccgattt
     9001 cttgtgccgc gacttggctg cgttcggcca gctttcttac ttcagtggcg accacggcga
     9061 agcctctgcc ctgctctcct gcgcgggctg cttcgatagc ggcgttgagg gcgagcaagt
     9121 tggtctgata agcgatctct tcgataatca taatcttctc cgcgatggtc tgcatggcat
     9181 cgacggattc cattaccgca gtaccggatt tttcgacgtt cctgacgccg gtcaaggcca
     9241 tctgttccat ctgccggctg atgtccgcat tttggctgat cgaggcgttc atttgctgca
     9301 gacttgaagt agtttcttcc accgcagcag ct
//
LOCUS       DASQKS010000254         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_557058, whole genome shotgun sequence.
ACCESSION   DASQKS010000254 DASQKS010000000
VERSION     DASQKS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557058"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1070)
                     /locus_tag="VE170_06640"
     CDS             complement(48..1070)
                     /locus_tag="VE170_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15150.1"
                     /translation="MANYKVIDADGHVRESNAGMREFLPPRWQRRNLFPNDEWDRDLR
                     GKLGQKPEGPKDQLAAMDEDGIDIMVMYPTAGLHVSALHEKDYATAVTQAYNDWLYHF
                     CKTDAARLKFVALLAPQDPSAAAEELRRAVTERGAVAGVLPTYLPQMPDFGDSFYDPI
                     YAAAESLGVGLGFHTGTSADSLGGQRFRKFLSAHTIDHPAEQMMALIATIVGGVFERF
                     TKLSIAYLESGIGWVPYMIDRLDEEVEKRGADEAPYLTKLPSEYVKSGRVYFGVECEE
                     KTIPDAMRWGLEDTILYSSDYPHWDGDWPHTVKGIRKRADLSDGVKMKMLHDNVARFY
                     HLDGAA"
     gene            1414..2586
                     /locus_tag="VE170_06645"
     CDS             1414..2586
                     /locus_tag="VE170_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYJ15151.1"
                     /translation="MLSHEDNDFFCRVGPDSPAGQLLRRYWHVVGAAAELNDAKPKKR
                     VRLLGENLLLYKDRSGGYGLVAENCSHRGVSLFYGFVEKDGIRCPYHGWKYDAGGHCV
                     DQPFENPEAGFKAKIQHPAYPVEKLGGLLFAYLGPPERQPLLPKWDLLVRNDGKKQID
                     ICQTLRCNWLQAMENSVDPTHTYYLHSHTLKLKGDPDHVPYHYRMLNKFDFEFVNYPT
                     WAGIQKQRIFEGDDVPVETPHPLIFPNILFVPVRIGYAMHFRTPIDDTNTQVYQFRFS
                     PSKDGSAVAQPESTPIEYVGTTDEAGEFHMDNFTSQDHMAWETQGPLADRSKEHLGES
                     DRGVVMFRKLLREQIQSVQNGADPIGVNFDPARDEVIKLIHENGYDAYSFAAARSS"
     gene            2596..>2825
                     /locus_tag="VE170_06650"
     CDS             2596..>2825
                     /locus_tag="VE170_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15152.1"
                     /translation="MSEGKPAAIVNDPLTPIPIPEQAPATQGIAELPGTRLGYWDTGG
                     NGTPVIFLHPASGSALVWLYQQPVFAQAGYRV"
BASE COUNT          620 a          840 c          761 g          604 t
ORIGIN      
        1 tagttttgcc atgtcaacct atcgctggtt attgttattg cgccgtctca cgccgcgccg
       61 tccaaatgat aaaaccgcgc cacgttgtcg tgcagcattt tcattttgac accgtcggaa
      121 agatcggcgc gcttgcggat acccttcacg gtatgcggcc agtcgccgtc ccaatgcgga
      181 taatcggagg aataaaggat cgtgtcttcc aagccccaac gcatggcgtc gggaatcgtc
      241 ttctcttcgc attcgacgcc gaagtagacc cgcccgcttt tgacatattc gctcggcagt
      301 ttggttagat agggcgcttc gtcggcgccg cgcttttcca cctcttcgtc aagccggtcg
      361 atcatgtacg gcacccagcc gatacccgac tcgagataag cgatgctgag cttggtaaag
      421 cgctcgaaca cgccgccgac gatggtcgcg atcagtgcca tcatctgctc ggccggatga
      481 tcgatggtat gggccgaaag aaacttgcga aagcgctggc cgccgaggga atcggcggaa
      541 gtgccggtgt gaaagccgag accgacaccg agactctcgg cggcggcata aatcggatcg
      601 taaaaactat cgccgaagtc cggcatctgc ggcaggtagg tcggcaacac gccggccacc
      661 gcgccccgct ctgtcaccgc gcgccgtaat tcttcagcgg ccgcggacgg gtcctggggc
      721 gccaataacg caacgaactt cagccgcgcc gcgtcggttt tgcaaaaatg atagagccag
      781 tcgttgtagg cctgggtcac cgccgtggcg taatcttttt cgtgcagcgc cgagacatgt
      841 agcccggcgg tcgggtacat caccatgatg tcgatgccgt cttcgtccat ggccgccagc
      901 tgatctttgg gaccttcggg cttctgcccg agcttgccgc gcaggtcgcg atcccactcg
      961 tcgttgggga aaagattgcg gcgctgccag cgcggcggca aaaactcgcg catgccggcg
     1021 ttcgattcgc gcacatgacc atcggcatcg atgactttat agtttgccat aatgactcct
     1081 tggtattccg tacatgatcc tcagataagc ggacgatgcg ccttggtgca gccataacac
     1141 ctaacaccgc gtaaaagagc ctgtcaatgc gcggcacatt ggcgaaacta cagagaataa
     1201 cgaattgagc tttttacctg tcctggaaca aagacgatga ctattcttat agaacatcgg
     1261 cttgcaggtc atttcccatc gcgtaaaact tcgccagtcc tgcctgatgc cgatcgcctt
     1321 gccgcaagtt gacacctggg caaaatttct aatagtgtcg aaaggcaaat tgccgctgcc
     1381 acccgccgga caaattgcca taaggagttg gtcatgctca gtcacgaaga caacgacttt
     1441 ttctgccggg tcggccctga cagccccgcc gggcaattat tgcggcgtta ttggcatgtc
     1501 gtcggcgccg ccgccgagtt gaacgatgcc aaaccgaaaa agcgcgtccg cctgctcggt
     1561 gagaacttgc tgttatacaa agaccgctcc ggcggttacg gcctggtggc ggagaattgt
     1621 tcccaccgcg gcgtgtcgct tttctacggc tttgtcgaga aagacggcat tcgctgcccc
     1681 tatcatggtt ggaagtacga tgccggcggc cactgcgtcg accagccttt cgaaaatccg
     1741 gaagccggct tcaaagccaa gattcaacat cccgcttatc ctgtggaaaa actcggcggt
     1801 ctgttgttcg cctacttggg tccgccggag cggcaaccgc tgttgcccaa atgggatcta
     1861 ttggtgcgca acgacggcaa aaagcaaatc gacatctgcc aaacgctacg ctgcaattgg
     1921 ctgcaagcga tggaaaattc cgtcgatccg acccacactt attatctcca ttcgcacacc
     1981 ctcaaactca agggcgatcc cgatcatgtg ccgtatcact accgcatgct caacaaattc
     2041 gattttgaat tcgtcaatta tccgacctgg gcgggtatcc agaaacagcg catctttgaa
     2101 ggcgacgatg tccccgtcga aacgccccat ccgttgatct ttcccaacat acttttcgtt
     2161 cccgtgcgga tcggttacgc catgcacttc cgcacgccca tcgacgacac caatacgcaa
     2221 gtttatcaat tccgtttcag cccaagcaaa gatggcagtg cggtcgcgca accggagtcc
     2281 acgccgatag aatatgtcgg aaccactgac gaggccggcg aatttcatat ggacaatttc
     2341 accagccagg accatatggc gtgggaaacc caaggccccc tcgccgaccg ttctaaagaa
     2401 cacctcggtg aaagcgaccg cggcgtcgtc atgtttcgca agttattgcg ggagcagatt
     2461 cagtcggtgc agaacggcgc cgaccccatc ggcgtcaact tcgatccggc cagggatgaa
     2521 gtgatcaagt tgatccacga aaatggctat gacgcatact cgttcgcggc ggcgcggtcg
     2581 agctgaggga ttagaatgag tgaaggcaag ccagctgcaa tcgtcaatga tccgttgacc
     2641 cctattccga ttcccgagca ggctcccgcg acccaaggaa tcgccgaact acccggcacc
     2701 cgtctcggct actgggatac cggcggcaat ggcacgccgg taattttttt acatccggcc
     2761 tcgggcagcg cgttggtctg gttataccaa cagccggtct tcgcccaggc tggttatcgc
     2821 gtcat
//
LOCUS       DASQKS010000255         5501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5588553, whole genome shotgun sequence.
ACCESSION   DASQKS010000255 DASQKS010000000
VERSION     DASQKS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5588553"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VE170_06655"
     CDS             <1..209
                     /locus_tag="VE170_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYJ15153.1"
                     /translation="LVSTKNSDMESVDDLKRRIDQASKYLPLEQLALSPQCGFGGIDS
                     KVLSEAEMWRKLDTIVETSKQVWN"
     gene            complement(240..521)
                     /locus_tag="VE170_06660"
     CDS             complement(240..521)
                     /locus_tag="VE170_06660"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HYJ15154.1"
                     /translation="MESMNDKFKIGDAVIVADENPKGNPRTPQYIRGKRGIIALAHGR
                     LENSRDHRGIHDPLYTVKFDLRELSACHDTDTIWVDVHEEWLSGARQDS"
     gene            complement(530..1603)
                     /locus_tag="VE170_06665"
     CDS             complement(530..1603)
                     /locus_tag="VE170_06665"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HYJ15155.1"
                     /translation="MSGKHGHRPFEGHMHDYMAHIKAVEEDLEYYRAKRFEPRIFYLL
                     RRNVVTFYDLLKVRSTLKKKDYFFNRTKPQGKNVEARVRYLEEWLDEYVKGIALVSAR
                     TVEGMDMVTEDNRKERGDYDEFFKIKKKEHHGTLEERMANLEQDLNDYQELLEVFVQA
                     LIKRGWATPEDLAQRWKQLHDERPWAGGVIVAKAWTDPEFKQALLDTGREALREMGVH
                     QGKVGKLLVVENSDKTHNVIVCTLCSCYPYDILGDTPWWYKHESYRTNIVQNPRACVK
                     EMFQLELPSDKELRVWDSTSDVRYFVLPQRPAGTEGMSEQELAKLVTVDSLIGAGYAL
                     EPSQLREREAAHAEPESPRVRPD"
     gene            complement(1629..3344)
                     /locus_tag="VE170_06670"
     CDS             complement(1629..3344)
                     /locus_tag="VE170_06670"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HYJ15156.1"
                     /translation="MADTPSNAKPRIAVFSGPTATIQNSEPLVTSNKAREKHRLPARL
                     NKDGSPMRFDVLRAQRLAAPVTVYVEQFSAHPLERDHAELYAPPDGFLDAAGKFHKER
                     TGPGDKPVYEITLKPEDGLYPLPYMARQVNGQPWEMDGTEKNIPADRVRVPFFPDGSR
                     LFEEIDRLGISDEGVGCLLTAKAAFDFYRALPSGGYATGRRESERTDIGTGDIAPEIR
                     GSDFFPYRPGYLRKEPPMAALAQLTNVVQQALRGGSYLGAIWLEGSPFVEETTYWLNL
                     LIDTTVPIVGNSSQRPHGAIGNDGDRNIVDSVNYITSKIWADDNGHDDIGAVAILDEQ
                     IFTARDVQKADARPGGYIATGGHGGIIGRMGHPGAPQFSFKPIKRHTHRSLVNLNRLP
                     RSVQGSTVQRSTIGIIAVPIKDAAGNLLAPAIPKVTIVKHARYLPEDTSGDASSEVDI
                     LARIENNLRISPLAGFVAEGSAPFGAMSNSVDAALKRATFSGMPVVKVGRGNAGGFVD
                     ATRDPLAIAGSNLTATKARLLLMACLLKLGCLPPAADPAKPTDAETDATKAKLKEYQE
                     IFDTH"
     gene            complement(3385..3963)
                     /locus_tag="VE170_06675"
     CDS             complement(3385..3963)
                     /locus_tag="VE170_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15157.1"
                     /translation="MSAVQKFIASARQLHADESDPAKRWEKMTPLLEELIADPAVREQ
                     SKQWPDCSQGGERAENLLFYEDPDYKFVINGLIKAPHTRTAIHDHAHNWTLYGVLDGS
                     ESIERYERIDDQSKPDYAEVRKSVNVKVSAGKIDLVPPYAIHAEESGDERTVAIIVRA
                     EKAGGFLQGRYDPATNKYWQGYGPKQLPFALK"
     gene            complement(4141..5409)
                     /gene="ahcY"
                     /locus_tag="VE170_06680"
     CDS             complement(4141..5409)
                     /gene="ahcY"
                     /locus_tag="VE170_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYJ15158.1"
                     /translation="MAGKKYDVKDMRMADGGRKRILWAGQDMPVLQRVKERFAKEKPL
                     RGMRFSACLHVTAETANLVQALKAGGADVVLCASNPLSTQDDVAASLVKHDKIPTYAI
                     KGEDNKSYYRHLRAALDHKPVITMDDGADLVSLLHTGYAHLLPNLVASMEETTTGVIR
                     LRALEKDGALKIPVIAVNDAATKHLFDNRYGTGQSTIDGVIRATDMLIAGKRVVVAGY
                     GWCGKGVASRARGMGAKVVVTEVDPVRALEAAMDGLEVMTMREAARVGDLFITLTGDK
                     HVIAEEHLKLMKSGAVVCNSGHFDIEIDIKALTKMATRVEKNVRNFVDAYVLPGGRRI
                     YLIGEGRLVNLAAAEGHPASVMDMSFATQALASEWAVKARKTLTPKVYEVPVAVENWV
                     AKLKLGSMGIRIDTLTKDQQHYLTSWDHGT"
BASE COUNT         1110 a         1598 c         1529 g         1264 t
ORIGIN      
        1 gcttagtctc gactaaaaac tccgacatgg aaagcgtcga cgacttgaaa cgacgcatcg
       61 atcaagcgag caaatacttg ccactggaac aactcgccct cagcccacag tgcggcttcg
      121 gcggcatcga ttcgaaagtg ctcagcgaag ccgagatgtg gcgaaagctg gatacgatcg
      181 tcgagacttc gaaacaggtt tggaattgac acaacctcga tcgattgctc tcctccgcgt
      241 cagctatctt gccgcgcgcc actcagccac tcttcgtgca cgtcgaccca gatcgtatcc
      301 gtatcgtggc aagcggaaag ctctcttaaa tcgaacttga cggtgtagag aggatcgtgg
      361 atgccacggt ggtcgcggga attttccaat cgcccgtggg ctagggcgat aatcccacgc
      421 ttcccgcgga tatattgggg cgtacgggga ttgcccttgg gattttcgtc ggcgacgatg
      481 accgcgtcgc cgatcttgaa cttatcgttc atcgattcca tgacttgtct caatcaggcc
      541 ggactctagg ggattccggt tcggcatgag cggcttcgcg ctcgcgcaac tgcgacggtt
      601 ccaatgcgta gccggcgccg atcaaactat cgaccgtcac caacttggcc aattcttgtt
      661 ccgacatgcc ttccgtcccc gcgggacgct gaggtaacac gaagtaacgc acgtcggagg
      721 tgctgtccca tacccggagt tctttatcgc tgggaagttc gagctgaaac atctccttca
      781 cgcaggcgcg tgggttttgc acgatgttgg tgcggtagct ttcgtgcttg taccaccagg
      841 gggtgtcgcc gaggatgtcg tagggatagc aagaacataa cgtgcagacg atcacgttat
      901 gggttttgtc ggaattttcg acgaccagca atttgccgac cttcccctga tgcacgccca
      961 tttctcgcag cgcctcgcgg ccggtatcga gcaacgcctg tttgaactcc gggtcggtcc
     1021 acgccttggc gacgataacg ccgccggccc atggacgctc gtcgtgcaac tgcttccagc
     1081 gctgtgccaa gtcttcaggc gtcgcccagc cgcgcttgat caaggcttgg acgaatactt
     1141 ccagcaattc ctgatagtca tttagatctt gttccaagtt agccattcgc tcttcgaggg
     1201 tgccatggtg ttctttcttc tttatcttaa aaaactcgtc gtagtcgccg cgctccttgc
     1261 ggttgtcctc ggtgaccata tccatgcctt cgacggtgcg cgccgacacc aaggcaatgc
     1321 ctttgacgta ctcatcgagc cactcttcca aataacgcac ccgcgcttcg acatttttcc
     1381 cctggggctt ggtcctattg aagaaataat cttttttctt gagggtcgag cgcaccttga
     1441 gcaaatcgta aaaggttacg acgttgcgcc ggagtaagta gaaaatccgc ggctcgaagc
     1501 gcttggctcg gtaatactcc agatcctctt cgaccgcttt gatatgcgcc atgtagtcgt
     1561 gcatatgacc ctcgaagggt ctatgcccat gttttcctga catcgaactt cctccgagtt
     1621 gctgcgcttc agtgggtatc gaaaatttcc tggtattctt ttaatttcgc tttggtcgcg
     1681 tcggtttccg cgtcggtcgg tttggccgga tccgccgccg gcggtaaaca acccaacttc
     1741 aacaagcacg ccattaacaa cagccgcgcc ttggtcgcgg tcaaattgct gccggcaatc
     1801 gccagcggat cgcgtgtggc atcgacaaaa ccgccggcgt tgccgcggcc gaccttgacc
     1861 accggcatgc cgctgaaggt cgcgcgcttt aacgccgcgt ccaccgagtt gctcatggcg
     1921 ccaaaaggcg ccgagccttc ggcaacgaag ccggcgaggg gcgagatgcg cagattgttt
     1981 tcaatccgcg cgagaatatc gacttcgctg ctggcgtcgc cggaggtgtc ctcaggcaaa
     2041 tagcgcgcgt gcttgacgat ggtaactttc ggaatcgccg gcgccaataa attacctgca
     2101 gcgtccttga tcggtaccgc gatgattccg atcgtcgaac gttgaaccgt tgaaccttga
     2161 acactgcgcg gcaaccggtt cagattcacc agcgatcgat gggtatgccg cttgatcggt
     2221 ttgaaagaaa actgcggcgc gccgggatgg cccatccggc cgatgatgcc gccatggccg
     2281 ccggtggcga tgtagccgcc gggacgggca tcggcttttt gcacatcgcg cgcggtgaat
     2341 atctgctcgt cgagaatcgc tactgcgccg atatcgtcat ggccgttgtc atcggcccaa
     2401 atcttcgacg tgatgtaatt caccgaatcg acgatattgc ggtcgccgtc attgccgatg
     2461 gcgccatggg gacgctgcga ggaattaccc acgataggca ccgtggtgtc gatcaacaga
     2521 ttgagccagt acgtggtctc ctcgacaaac gggctgcctt ccaaccagat ggcgcctaaa
     2581 tagcttccgc cgcgcaaagc ttgctgaacg acattggtca actgtgccag cgctgccatg
     2641 gggggttctt tgcgcagata accaggacga tagggaaaga aatcactgcc acgaatctcg
     2701 ggcgcaatat cgccagtgcc gatatcggta cgttcgctct cacgccgacc ggtcgcgtaa
     2761 ccgcctgacg gcagtgcgcg ataaaagtcg aatgcagctt tggctgtgag caaacagccg
     2821 acgccttcgt cggaaatacc caatcggtcg atctcttcaa acaagcgcga gccgtcggga
     2881 aagaacggga cgcgcacccg atcggcagga atattttttt ccgtgccgtc catctcccat
     2941 ggctgtccgt taacctggcg cgccatgtac gggagtgggt agagcccgtc ttcgggtttt
     3001 agcgtaatct cgtaaaccgg cttgtcgccg ggaccggtcc gttccttgtg aaacttaccc
     3061 gccgcgtcga gaaagccgtc cggtggcgca tagagctcgg catggtctct ctcgagcggg
     3121 tgggcgctga attgctcgac ataaaccgtc accggcgcag ccaagcgctg cgcgcgcagc
     3181 acgtcgaaac gcatcggcga gccgtctttg ttcaggcggg ccggcaggcg gtgcttttcg
     3241 cgcgccttgt tactggtcac caacggctcg ctgttttgaa tcgtcgccgt cgggccggaa
     3301 aagaccgcga tgcgcggttt cgcgttgctc ggtgtgtctg ccatcatgaa ctcgctaagc
     3361 tagaatgagc gaaatcaact gatctcactt aagcgcgaac ggcaactgct taggtccgta
     3421 gccctgccaa tatttgttgg tcgccgggtc gtagcgtccc tggaggaaac cgccggcctt
     3481 ttcggcgcgt acgatgatcg ctacggtccg ttcgtcgccg ctctcttcgg catgaatcgc
     3541 atagggcggc actaggtcga tcttgccggc actcactttg acgtttaccg atttgcgtac
     3601 ctcggcgtag tctggcttcg actggtcatc gatccgttca taacgctcaa tggattcgga
     3661 accgtcgagg acgccgtaaa gcgtccaatt atgggcgtgg tcgtggatcg ccgtgcgcgt
     3721 gtgcggcgcc ttgatcaagc cgttgatgac gaatttgtag tcgggatcct catagaaaag
     3781 taggttttca gcgcgctcgc cgccctgaga acaatcgggc cactgcttcg actgttccct
     3841 caccgccgga tcggcgatca gctcctccag gagaggggtc atcttctccc agcgttttgc
     3901 cggatcgctt tcatcggcgt gaagctggcg tgcggacgcg ataaactttt gcactgcaga
     3961 catgggacct ccttcagcat aatgccaatt gtcatgagct agcgaattat tagtccgatg
     4021 cgatactgct cgtcaagctg ggctgaaaga tttcaaatta cagattccaa attacaaaat
     4081 aaaaaaggct ccgacagcga tcgagccggc agagcctttg gaatcaataa caaatcgagc
     4141 ttatgtgccg tgatcccaag aagttagata gtgctgctgg tctttggtga gagtgtcgat
     4201 gcgaatgccc atggagccaa gttttaactt cgcgacccag ttctccaccg ccaccggcac
     4261 ttcgtagact ttcggggtga gagtcttccg cgctttcacc gcccactccg aagctaatgc
     4321 ctgagtcgcg aagctcatat ccatgaccga agccgggtgg ccttcggccg cggccaggtt
     4381 caccagtcgg ccttcgccga tcaagtaaat tcttcggccg cccggcagca cgtaggcgtc
     4441 gacgaaattg cggacatttt tctcgacccg cgtcgccatc ttggtcagcg ccttgatatc
     4501 gatctcgata tcgaagtggc cggagttaca gaccaccgcg ccgcttttca tcagtttgag
     4561 atgttcttcg gcgatcacat gcttatcgcc agtcaaagtg atgaagagat cgccgacccg
     4621 cgccgcttct ctcatggtca ttacttcgag tccgtccatg gcggcttcca aggcgcgcac
     4681 cgggtcgact tcggtgacaa ctactttggc gcccatgccg cgggcccgcg acgccacccc
     4741 tttgccacac caaccgtagc cggcgaccac tacgcgtttg cccgcgatca gcatatcggt
     4801 agcgcgaatc acgccatcga tggtcgactg gccggtgcca tagcggttat cgaataggtg
     4861 cttggtcgca gcgtcgttga cggcgatgac gggaatcttc aatgcaccgt ctttttccag
     4921 agcgcgcaaa cgaatcacgc ccgtggtggt ctcttccatg ctcgccacta agttgggcag
     4981 caaatgggcg tagccggtgt gcagtaaaga aaccaaatcg gcgccgtcat ccatagtaat
     5041 gactggcttg tgatcgaggg cggcgcgcag atggcggtaa taggatttgt tgtcttcgcc
     5101 tttgatcgcg taggtcggaa tcttatcgtg cttcaccaac gatgccgcga catcgtcctg
     5161 ggtcgatagc ggattggaag cacagaggac cacgtcggcg ccgccggctt tgagagcttg
     5221 caccaagttc gcggtctcgg cggtgacatg caagcaagcg gagaaacgca tgccgcgcaa
     5281 cggtttttcc ttggcgaaac gctctttcac ccgctgcagc actggcatat cctggccggc
     5341 ccagagaatc cgcttacgcc caccatcggc catgcgcata tcttttacgt cgtatttctt
     5401 tcctgccatt ttgtcgattt cctctcgtca gtttgtcgta ataaaaaatt agttcagcaa
     5461 ttaccggcaa cacaaagcgt gcgcttagcg caccctgcga g
//
LOCUS       DASQKS010000256         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5589197, whole genome shotgun sequence.
ACCESSION   DASQKS010000256 DASQKS010000000
VERSION     DASQKS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5589197"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1378)
                     /locus_tag="VE170_06685"
     CDS             complement(116..1378)
                     /locus_tag="VE170_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15159.1"
                     /translation="MPSETKGLQFLVRALGSRNFRLFFFGQAVSLIGTWMQLIAMRWL
                     VYRLTKSELMLGMVGFISDAPLFLLVPFAGVLADRLKRHRIMVVTQALSALQAALLAL
                     LVLTDQVAVWHVLVLGGLLGVVSAFDITARQAFIVDIIEERENLPNAIALNSFIFNGA
                     LLIGPAVAGILIAAVGEGPCFALNSLSYLAVLGALFLMKIPEKTFSASPLNLQAAIIE
                     GATYTFASVPIRSILMLVAAVSLVAASYTLLMPVFAEDILRGGPRVFGYLMSATGVGA
                     LGGAIFLASRRRLKGLAEIIVIAGAIYGVGLIALSFTRSLAMALVIALALGVSLMMQM
                     ASSNTIVQSIVDDQKRGRVMSLFVMARRGVESLGSLLFGVIAHWVGTPRTLMIGGAFS
                     VLAVAAFAANLRSIRQASRTVDSQSKRQ"
     gene            complement(1509..2369)
                     /locus_tag="VE170_06690"
     CDS             complement(1509..2369)
                     /locus_tag="VE170_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYJ15160.1"
                     /translation="MIRFDYQEPTTLKKVFALMEKHGDDGRVMAGGTSLIIMMRQRLL
                     TPKVVISLARLPKFDKISYSANDGLRIGAGARHRDIELSKAVQQHYPLLHETFRKVAQ
                     PRIRNMGTVGGNLAAGDPLTDPGASLIALDAEVTLTSSKGQHTLRLDEFFIDYYQTAM
                     EPGELLTEIHVPPATRQGWSHIKFTPRSVEDFATVGVAITVRTMDGICEDVRLGLNSV
                     SSTIVHAQKAEAILRGKPINDERLREMGEIAASECDPTDDNRGSAEYKRELVKVLVPR
                     AANEAIARAG"
     gene            complement(2424..>2848)
                     /locus_tag="VE170_06695"
     CDS             complement(2424..>2848)
                     /locus_tag="VE170_06695"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ15161.1"
                     /translation="AAQVHVDPQTGQVKVLQMVVASDCGTVIYPIGAEGQVEGGLAQG
                     IGYALTEGLQIDEGRPLNPNFSDYRIPSMRDMPPLQHAFADSYEPTGPFGAKGLGELN
                     MDPTAAVINNAIFDAVGVRVKTLPITPEKILRALKEKK"
BASE COUNT          622 a          782 c          850 g          594 t
ORIGIN      
        1 ttcgagtttt taacagaggg tgcaagccgc tcattactac tcgaaaagat ctttcaccgg
       61 aaatcagtca ttggactacc aagagagctt ggagtgggct cagttatctt cacgtttatt
      121 ggcgtttgct ttggctatcg accgtgcggc ttgcttgtcg gatcgatcga agattagcgg
      181 caaaagcagc caccgcaagc acagagaacg cgccaccgat catcaaagtc ctgggcgtgc
      241 caacccagtg ggcaatgacg ccgaacaata gactgcccaa ggactcgacg ccgcggcgtg
      301 ccatgacaaa gaggctcatg accctgccgc gcttctggtc gtcaacgatg ctctggacga
      361 tggtgttgct cgatgccatc tgcatcatca aggagacccc tagcgccaga gcgatgacca
      421 atgccatcgc cagcgatcgc gtgaacgaca aagctatcag gccgacgccg tagatggcgc
      481 cggcgatcac aataatctcg gccagccctt taagacgcct gcgtgaggcg agaaatatgg
      541 cgccgcccag agcgccgacg ccggtggcgg acatcaggta gccgaagact cgagggccgc
      601 cgcgcaagat gtcttcggcg aataccggca tgagtagcgt ataggaagcg gcgacaagac
      661 tgaccgccgc aaccagcatc aagatggatc ttatgggcac agaagcgaag gtgtacgtag
      721 cgccttcaat gatcgccgcc tgcaaattaa gcggactggc ggaaaatgtc ttttccggga
      781 ttttcatcag gaacagcgcg cctaacaccg ccagataact caagctattg agggcaaaac
      841 agggcccttc cccgaccgcg gcgattagga tgccggcgac ggccgggcca ataaggagtg
      901 cgccgttaaa aatgaaagag tttaatgcga tggcattggg aaggttctct cgttcctcga
      961 tgatatcgac aatgaaggct tggcgcgcgg taatatcgaa ggcgctgacg acgccgagaa
     1021 gcccgccgag aacgagcacg tgccagaccg ccacctgatc ggtgaggacg agcagcgcca
     1081 acagcgccgc ctggagcgcc gaaagagctt gggtaacgac catgatgcgg tgacgtttga
     1141 gccggtccgc caaaacgccg gcaaagggca cgagcaaaaa gagcggtgcg tcgctgataa
     1201 atcccaccat gcccaacatc agttctgatt ttgtcaaacg atagaccagc cagcgcatgg
     1261 cgatgagctg catccaggtg ccgatcaatg acaccgcttg gccgaagaaa aacagccgaa
     1321 agttgcgcga gcctaaggcg cgcacgagaa actgcagacc tttggtttcg ctgggcatca
     1381 agtgactttc gtgattgcga attctacccg gccgctaggc tcagcgcacg ctccctgggt
     1441 cgaattccca cagcttgctg cgtggtatgc caagacccgg tggtcgggaa attatttggt
     1501 gagaaggctc aaccggcgcg cgctattgct tcgttcgcgg cgcgggggac caagactttc
     1561 acaagctcgc gtttgtactc ggcagagcct cggttgtcgt cggtgggatc gcattcgctg
     1621 gcggcgatct cgcccatttc gcgcaatcgt tcatcattga tgggtttgcc gcgcaaaatc
     1681 gcttcggctt tctgagcgtg aactatcgtc gaggaaaccg aattgaggcc gagccgcaca
     1741 tcctcgcaaa tgccgtccat tgttcgcaca gtgatcgcca caccgaccgt ggcgaaatct
     1801 tcgacactgc gcggcgtgaa tttgatatgc gaccagccct ggcgggtcgc cggcggaacg
     1861 tggatttcgg ttaacaattc tccgggctcc atggcagttt gatagtagtc gatgaaaaac
     1921 tcgtccaacc gcagtgtatg ctgccccttg ctactggtta gcgtcacctc ggcatcgaga
     1981 gcgatcagac tggctcccgg gtcggtgagt ggatcgccgg ccgcgagatt gccgcccacc
     2041 gtgcccatgt tgcggatgcg cggttgggcg actttacgaa aggtttcatg gagcagggga
     2101 tagtgctgct gcacggcttt cgatagctca atgtcgcgat ggcgcgcacc ggcgccaatg
     2161 cgcaggccgt cattggcgct atagctaatc ttgtcgaact tcggcagtcg cgccagacta
     2221 attaccactt tgggtgtgag caggcgctgg cgcatcatga tgatcagcga cgtgccgccg
     2281 gccatgacgc ggccatcgtc gccatgtttt tccatcaagg cgaagacttt tttgagcgtc
     2341 gtcggttctt ggtaatcgaa gcggatcata ttgcaacttg agttgtttta ttgcgaattt
     2401 tgcatagttg gatcatggcg aattcacttc ttttccttga gggcgcggag gattttctcc
     2461 ggcgtgatcg gcagggtttt tacccgtacg ccgacggcgt cgaagatggc gttattgatg
     2521 accgccgcag tggggtccat gttcagttcg cctaatcctt tggcgccaaa cggccctgtg
     2581 ggttcatagg agtcggcgaa agcatgctgc agcggcggca tgtcgcgcat ggagggaata
     2641 cgatagtcgg aaaaattggg attgagcggc cggccttcgt cgatctgcaa accttcggtg
     2701 agggcgtagc cgatgccttg cgccaaaccg ccttcgacct ggccttcggc gccgattgga
     2761 tagatcaccg tgccgcagtc ggaggcgacg accatttgca acactttcac ctgtccggtt
     2821 tgcggatcga catggacttg cgcggctt
//
LOCUS       DASQKS010000257         6279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_557982, whole genome shotgun sequence.
ACCESSION   DASQKS010000257 DASQKS010000000
VERSION     DASQKS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557982"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1307)
                     /locus_tag="VE170_06700"
     CDS             complement(273..1307)
                     /locus_tag="VE170_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15162.1"
                     /translation="MNLRRMYLPLIVTVGILTLPTTPVHGAAADREKILEGAKKEGRL
                     VLYTGMETDESSLFTQEFARKYPFIKPEIFRSSGEKVQARFMVEHRANTHLADVFQTS
                     IVQVYQLKNSALLARYVSEEAPAYADGFKDGQGFWTAFYQIPYVIGYNTRLVAAKDVP
                     ASYEDLLNPKWKGQISLETEEYQWFYHLLQIMGRDKGLDFMKKFAGQNLQMRKGHTLL
                     AQLVAAGESALATVVYSNRVERMKTTGAPIDWVRFKGPTITAINAIAIPDKAPHANAA
                     RLFVDFVLSREGQSLLRGLRRIPARADVAPDPPSLTKGLNFYPARPEGMIENFTDTVA
                     RFDEIFNKGR"
     gene            1593..2765
                     /locus_tag="VE170_06705"
     CDS             1593..2765
                     /locus_tag="VE170_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15163.1"
                     /translation="MPDFKLISADSHVNEPPAAWERVQKEYGERAPKVVRDPAGKPKG
                     IWLIIDGLPPVGLSHYSKGLAVGKNRGISEVEQEKHFATIRFNENFRYEDYPGGWEPS
                     ARLQDQEADGVEAEVLFSSAVRQLYSIVDEPFQRAIFHSYNAWLHDFCSASPNRLLGL
                     ALIPILDMEHTVADIYHYAKLGFRGVQIPTRIKDSGYYDAKYEPMWRALEETGMIVNV
                     HTSVTQGVARTHYEGPREEDPVKEPIGFARRQVPAQQFVGNMILSGNLDRHPKLRVVC
                     AEFDIGWVANMVQQVDYWFGRESTFDADRNLNQRPPSEYFKSNIFFTYQDDRAGVLTT
                     PVYGEDNFLWASDYPHGVTTWPFSKDAVDRNCEGIDPKIKRKLGRDNAAKLYGLGD"
     gene            complement(2924..4939)
                     /locus_tag="VE170_06710"
     CDS             complement(2924..4939)
                     /locus_tag="VE170_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HYJ15164.1"
                     /translation="MIMGKRRVLIVAGSVFGMAAIALAIFIATLDQNQAKKYISAGVS
                     KVTGRQLRINGDLKLDLGWIFTVSANQIQFENAGWSKHAQMAEIGNFDVQIDLWQLLT
                     KFRLVIPTVTISQAKITLEKNAAGSANWEFRAAPTGPQKRSEFPVIEKLVIKDGMLSF
                     DNQETKTQIELKVSEAEGAGFLERPVKLKVAGTYQKLPLTIALDGGTYENLRSSKTPY
                     PLRIDLGAGKLKAIIDGNLTDPLEMKREDVSLDIQGDDLAKLYPLIHVVFPATPPYRL
                     KGRLKHDGNVWSFSNFSGRVGGSDLSGSIQVDTAPKRLFMKADLVSTLLDFNDLAGFI
                     GGKPGTAPDKTALVEPNKQTAAEKDSERIFPDQPYDLERLRAMDADVRLRAKKILAAK
                     LPIDDLNATLTLTDGVLSFAPATFGVANGRVEIFSTFDGSNRPSKVKIDARLRQLELQ
                     RFLSKGSLAQKTVGPIGGRIVLTGSGQSFRELMATATGNSFFTMSGGQISELLVELAG
                     LDIAHALGVVVSGDKPIPIRCALLDLQGQNGQMAVQTLVFDTANSIISGEGNIDLRDE
                     KVDIVVIPAPKRFSPLSLRSYIRVAGGFKNISVFPDPIKTGTDSLVKKVFNLLTLLVT
                     SPVQPRDLWQGKDADCDALIASVHKQDPRGVVLKDVQKPGIPSISAK"
     gene            complement(5690..5839)
                     /locus_tag="VE170_06715"
                     /pseudo
     CDS             complement(5690..5839)
                     /locus_tag="VE170_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
BASE COUNT         1430 a         1781 c         1657 g         1411 t
ORIGIN      
        1 gtccaagccg cgcccaaagt cttcaggttg gacgccaaaa tgatcgccga agtgatcgac
       61 gccaaagccg acaccgacga aaagatagtg cttactgcga agatctgccc gacaaaggca
      121 attattctgg aggaagaaga gacgggcaaa agaattttcc cggtggaatg actaacggcg
      181 ttggtcggag acgatcagaa gccacagcaa cgcgtcgcct ggttctaccg ccgtgctcgg
      241 tattgtagaa cgaacccgat ctcgccctcg gactatcttc ctttattaaa aatctcatcg
      301 aagcgcgcca cggtgtcggt gaagttctcg atcatgcctt cgggacgcgc tggataaaaa
      361 ttcaaccctt tggtcagact cggcggatcg ggcgcgacat cggcgcgcgc cgggatgcgc
      421 cgcaggccgc gcagcaggct ctgcccttct ctggataata cgaagtcgac aaacaaacgc
      481 gccgcgttgg catggggcgc tttgtcaggt atggcaatcg cgttgatagc ggtgatcgtc
      541 ggccctttga aacgaaccca atcgatcggc gcgccggtcg ttttcatgcg ttcgacgcga
      601 ttggagtaaa ccaccgtggc cagcgccgat tcgccggccg cgaccagctg cgccaacaat
      661 gtgtggcctt tgcgcatttg caaattttgc ccggcaaatt ttttcatgaa gtcgagcccc
      721 ttgtcgcgcc ccatgatttg cagcaaatga taaaaccact gatactcctc ggtctctaga
      781 ctgatctgcc ccttccattt cggattcaac aaatcttcgt aactcgccgg cacatccttg
      841 gccgccacca ggcgcgtgtt gtagccgatt acgtagggaa tttgatagaa cgcagtccaa
      901 aagccctgcc catctttgaa gccatcggcg taggccggcg cttcttcgga aacataccgc
      961 gccagcaggg cggagttctt gagctgataa acttgcacga tgctggtctg aaaaacatcc
     1021 gccaaatgag tgttggcgcg gtgttcaacc atgaagcgcg cttgcacctt ttcaccgctg
     1081 gagcgaaaaa tttccggctt gatgaaggga tacttcctgg cgaattcttg ggtgaacaga
     1141 ctcgactcgt cggtttccat accggtgtat agaaccaacc ggccttcttt tttcgcccct
     1201 tcgaggattt tctcccggtc agcggcggcg ccatggaccg gcgtcgtcgg caaggtcaaa
     1261 atccccacgg taacgataag cggcaagtac attctgcgca ggttcatcgt tcattctcgc
     1321 gccagtggca ctttggccgc agcatagcgc caatccaccg gctcttgtca acattccccc
     1381 gaggcccggc ggcggccaac ttgacaatcc ggcgccgact cccctatcgt cgcctaaaaa
     1441 tattcatgct ttacacaacc gagccctacg atccgctacg gcctcgaccc gcgttcgaca
     1501 tcaggcgatg tcgatcttcg caccggcaac ggcaccaagg acaggtcgcg ccactgtata
     1561 tgtatgtaga gaattattga ggagcgacgc tcatgccgga tttcaaactg atttccgccg
     1621 atagtcatgt gaacgaaccg cccgccgctt gggagcgggt gcaaaaagag tacggcgagc
     1681 gcgcgccgaa agtcgtccgc gacccggccg gcaagcccaa gggcatctgg ctcatcatcg
     1741 acggcttacc gccggtcggc ctatcgcatt attcgaaggg cttggccgtc ggcaagaatc
     1801 gcggcatcag tgaagtcgag caggaaaagc atttcgcgac cattcgcttc aacgaaaact
     1861 ttcgctatga agattacccg ggcggctggg agccgagcgc gcgcttgcaa gatcaggaag
     1921 ccgacggcgt cgaggccgag gtgctgtttt caagcgccgt ccgccagctc tacagcatcg
     1981 tcgacgagcc gtttcagcgg gcgatctttc actcctacaa cgcatggctg cacgatttct
     2041 gtagcgccag tccaaaccgc ttgctcggct tggcgctgat tccgatcctg gacatggaac
     2101 ataccgtggc cgatatttat cattacgcca aactgggttt tcgcggcgtg cagattccga
     2161 cgcggatcaa agacagcggc tattacgacg ccaaatacga gcccatgtgg cgggccctcg
     2221 aagagaccgg catgatcgtc aacgtccaca ccagcgtcac ccaaggggtt gcgcggaccc
     2281 actacgaagg gccgcgtgaa gaggacccgg tcaaagagcc catcggtttt gcgcgcagac
     2341 aggtgccggc ccaacagttt gtcggcaata tgattttgtc cggaaacttg gaccgtcatc
     2401 cgaaactaag ggtggtctgc gccgagttcg acatcggctg ggtggccaac atggtccagc
     2461 aagtcgacta ctggttcggc cgcgaaagca ccttcgacgc cgaccgcaac ttaaatcaac
     2521 ggccgcccag cgaatatttc aaatcgaata ttttcttcac ctatcaagac gatcgcgccg
     2581 gcgtgttgac gactccggtt tacggcgaag acaattttct ctgggccagt gactacccgc
     2641 acggggtgac aacctggccg ttctcaaagg acgccgtcga tcgcaactgc gaaggcatcg
     2701 accccaagat caaacgcaag ctcggccgcg ataacgcggc caaactctat ggcttaggtg
     2761 attgagcaaa cgccaaagtg atcgacacaa gagccgacac cgacgagaaa attcttatcc
     2821 ccgccaactg tgcccgacca aagacactag tttggaacag gagggaagag gatcgaaatt
     2881 tttccggtgg attgacctgc gtcgccggtc actgtgcgaa ccgtcactta gcagaaatgc
     2941 tggggatacc cggcttttga acgtccttga gcacgactcc gcgcggatcc tgtttgtgaa
     3001 cactggcgat cagtgcatcg cagtcggcat ctttcccctg ccagaggtct ctcggttgta
     3061 ctggactcgt aaccagcaac gtcaaaagat taaagacctt ttttaccagc gaatcggtcc
     3121 cggttttgat cggatccgga aagacggaga tattcttaaa acctccggcc acgcggatat
     3181 atgaccgcaa actcagtgga ctgaaacgtt tcggagccgg tattacgaca atatcgacct
     3241 tctcgtcccg gaggtcgatg ttaccttctc cggaaatgat ggaattggcg gtgtcgaaga
     3301 ccagggtttg aacggccatc tgtccgttct gcccctgcag atctagcaaa gcacaacgga
     3361 tagggatggg cttgtcgccg ctcacaacca cgcccagcgc gtgagcgata tcgagtccgg
     3421 ccaactcaac aagcagctcg ctgatctggc ctccagacat cgtaaagaac gagttgccgg
     3481 tggcggtcgc catgagttcg cgaaatgact ggccggagcc cgtaagaacg attcgacctc
     3541 caatcggccc tacagttttt tgggcgagag aacccttgga aaggaatcgc tgcaattcca
     3601 actgtcggag ccgagcatcg atcttgacct tggagggacg attcgatccg tcgaacgtgc
     3661 tgaagatttc cacgcggcca ttggcgacac cgaacgtagc cggcgcgaag ctcaaaactc
     3721 catcagtcag ggttaacgtt gcgttcagat cgtctatcgg cagcttggcc gcgagaattt
     3781 ttttagcacg caggcgaacg tccgcatcca tggctcgcaa gcgctcgagg tcataaggtt
     3841 gatcaggaaa tatccgctcg ctgtcctttt ctgcggcagt ttgcttattt ggctcgacta
     3901 aagctgtctt atccggagct gttcccggct tgcccccgat gaaaccggct aagtcgttga
     3961 agtcgagtaa ggtagaaacg aggtcggcct tcataaacag gcgcttcggc gctgtatcca
     4021 cctggatact gccggagaga tcactaccgc ccacccggcc ggaaaaattt gagaatgacc
     4081 agacattgcc gtcgtgcttt aaacgaccct tgaggcggta aggcggcgtc gctggaaaca
     4141 cgacgtggat gagcgggtag agtttcgcca ggtcatcgcc ttgaatatca agtgagacat
     4201 cctctcgttt catctccaac gggtcggtga gatttccgtc gattattgct ttcagcttac
     4261 cggcgccaag atcgatccgc agggggtacg gcgtctttga acttctcaag ttttcgtatg
     4321 tacctccgtc caaggctatg gtgagcggca gtttttggta ggtgcctgcc actttgagct
     4381 tgaccggccg ttccagaaaa cctgcgcctt ccgcctccga caccttcaat tcaatctggg
     4441 tctttgtttc ctgattatcg aatgagagca tgccatcctt gatgaccagt ttttcgatga
     4501 ccggaaattc actgcgtttc tgcggccctg taggcgccgc gcgaaactcc cagtttgccg
     4561 agccggcggc atttttttcc agagtgattt tcgcttgcga gatggtgacc gtgggaataa
     4621 ccaagcgaaa cttagtgagg agctgccata gatctatctg aacgtcgaag tttccgattt
     4681 cggccatctg tgcgtgtttg ctccagccgg cgttctcgaa ttgaatctgg ttggcgctca
     4741 ctgtgaagat ccaaccgaga tccagtttga gatccccatt gatgcgcaat tgccggcccg
     4801 taaccttgct cacaccggcc gaaatatatt tcttcgcttg gttctgatcg agcgtcgcaa
     4861 taaaaatcgc cagcgcaatc gcggccatgc caaagacaga ccccgccaca atcaaaaccc
     4921 ggcgtttccc cataatcatg attaaccctc acctgcacac agtggagtca tggaattacg
     4981 caagagacgg accacggctt tgcaatcact acaaagccgc caaggagaaa ttttgccggc
     5041 gatttttcga atgctagaac ctctgggctg gaaatcttag aaaatggtag gagtttcctt
     5101 gatcaaatcg agctgacatc gcttaaacca gctaatctta gcgtgaaccg agttttttaa
     5161 gttcctcaat cttagggtga agcagcgtcg acgccaacaa cgaactcgac catttcgtcg
     5221 actcgatgat cccatcccgc gcgggcagtc gatgaccatt gaactcccct ttttattttt
     5281 tgcgacgaag gcgaacaagt gacttgcgtt gaaggtttat cgagggctga gtttcacctt
     5341 caatctaata gatagggttg gaaacggctt gcgccgtggg tcacaccttc agcgcgccag
     5401 cgtcggcacc gggctagaat cccagctgga acatcatcca gatgaaaaat catcccactt
     5461 agtgaaatat cagtctgtca tcggacgccg tttcgggacg atgatgtgtt cacttctctt
     5521 cgcgcctgtc gcgcacgagt tgaatctctt ccaagcaacc ggcgcacata tatgccgcgt
     5581 ggggcaggaa aatcagctca tcggtttcgc gcgcttccat gcagatgccg cagaggcgct
     5641 cttcggccac tcccgcggcg gctttcaact ttgtttccgc ctcgtcgaca ttttccttgg
     5701 tgaggatttc gacgcagctg cgcacgcatt cgttgcaaat atggacggat gggccggcga
     5761 tcagcttgcc cacctcattt tgatttttgc cgcaaaacgc acatgtcagc agtttgttat
     5821 cgtcggcaaa ctggggtatt tgcgactccc atttgtcgcc gaaggcgtgt ttcagcgttt
     5881 cttccaaagt cgaaccgatt tgaaaaagct gccgggcgat ctcactgcgc ttcggcgtcg
     5941 acttcgaatc gaggaaattc gccaccgctt gctgaaaggt ttttaacgcg gcgccatact
     6001 tctgcccgtt gatcaaatcg gcgcattcga caaacccgaa accgcttatt tttgtcgcca
     6061 tgacgacccc ttccacgtcc actgtttcca ttgccgccgg atatcccggc aatcgaccac
     6121 ccgaccgtag cacaatttcc gcctgcggac agtaagccca atccgtgggt tggaactatt
     6181 tcaagagaca ggaatggaat caccctgcgc ttggagattg atcgaatacc gggtactcaa
     6241 gttgcaattt tcgcagcggc gtcgaagtca agaaaactg
//
LOCUS       DASQKS010000258         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_558046, whole genome shotgun sequence.
ACCESSION   DASQKS010000258 DASQKS010000000
VERSION     DASQKS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558046"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VE170_06720"
     CDS             <1..1204
                     /locus_tag="VE170_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15165.1"
                     /translation="AYGERGLLFQEETSIPLPARREAKMPAQVAGGVTMVTTEQRKII
                     DADGHVAEDSAAIVAHMPEAYRAKAKLQPFNPFPPFDHLHAGHLVDMPPGSFNRKVGP
                     KEWLAFLDEVGIDTTVLYPTAGVASANIVNLDWAVDATRAYNDWLYETYLQHSPRFRG
                     LALLPQALINPQAAADELRRAVKQLGMCGAVMPSNSLHAPPLGTATYLPVYQVANELG
                     CCVAIHGGVHGHMGMDGLDPYAAVHAIGHPLGQMISLASLVFNGVLDRFPKIRFGFLE
                     GGVAWLLFCLERFDRSYDSHVMRDLRGSYLKLQPAEKISDYIIRHVKARRIFVGCEGG
                     EPLLATAVKMVGNDPFMYSSDFPHEVNAATCKKELAELVENPELSATDKGAILYQNAI
                     QFYGIREN"
     gene            complement(1367..2044)
                     /locus_tag="VE170_06725"
     CDS             complement(1367..2044)
                     /locus_tag="VE170_06725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ15166.1"
                     /translation="MIQSLLSLSLLFLGFISTTLYAQDAAKRNQQQMHGLHNDPKAYM
                     KALEDPKRDEYQKPHEVLKALNIKPGETIADIGAGTGYFTFPLAHHVGDKGKVYAVDV
                     SADMIRHINRRIRETKSANVVSILADLDDPLLPDRSVDRFFICDVWHHVENQTKYLTL
                     MKKMLKPGGEIVMIDFHKKELPFGPPLQMKIAREDLIKQMETNGFRLSKEHKFLPHQY
                     FLVFMPN"
     gene            2242..3687
                     /locus_tag="VE170_06730"
     CDS             2242..3687
                     /locus_tag="VE170_06730"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HYJ15167.1"
                     /translation="MGELVDYQIAIDRRRNKRGSWKHARERMLNEVWPVITPSFELTP
                     GGKIFTIGSCFARNIEEHMQRLGFEIPMLKFRVPAEEWGARANGILNKYTPAAIFQEI
                     DWTKNIMVRDGVVRESDCAAFRFDCDGDFCVDTNLAGFNLVSRQRYFERRCQIYEVFK
                     EVFTADCVVITLGLVEAWLDREKGIYIQEVPSGKIFAKQARRFAFESLSYEKCRDYIQ
                     RSVDTVREVNKNAKILITTSPVPLSRTFTTDDVIIANSHSKSVLRAVAGEIAAANVAM
                     DYFPSYESVSGTKSWQIWGEDLLHVSDAFVGKVVSRLTDIYCPGIQQSRKKYLDFYMA
                     SKGDDLSEALELGEQLSETNSVEYLERLSQVLERKGDINAAVHELKKALDQVPAEPRL
                     RFQYSKLLAQSGRMQDALSEARYGVALDPTNLRYRRHLVTLYARDRNYVKALGQRVLA
                     ALYRRQSKTQNVTLKRLLQLVCHQGITAQKS"
     gene            3881..>4248
                     /locus_tag="VE170_06735"
     CDS             3881..>4248
                     /locus_tag="VE170_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYJ15168.1"
                     /translation="MAQTELKRQIGLFSAVMLIAGDMIGTGIFISTGAIAETLPSPGG
                     VLLVWLFGGLLALAGALTCAELAASLPYAGGDYVYIREAYGRLMGFLSGWSSFLVTFS
                     GAIAFLAVILNGFVSFFIPV"
BASE COUNT          907 a         1101 c         1228 g         1012 t
ORIGIN      
        1 tgcgtatggc gagcggggtt tgctatttca agaagagaca agtattcccc ttcccgcgcg
       61 acgggaagcc aaaatgccgg cgcaggtcgc aggaggtgta accatggtga caactgaaca
      121 gcggaaaatc atcgacgcag acggccacgt cgccgaagat tcggcggcga tcgtcgcgca
      181 catgccggag gcttatcgcg cgaaggcgaa attgcagccg ttcaatccgt tcccgccgtt
      241 cgatcatttg catgccggcc atctggtcga catgccgcca gggtcgttta accgcaaggt
      301 tgggccgaaa gaatggctgg cgttcttgga cgaagtcggc atcgacacga cggtgctcta
      361 tccaaccgcg ggcgtggcct cggccaatat cgtcaacctc gactgggcgg tcgacgcgac
      421 gcgggcgtac aacgactggt tgtacgagac ctatttgcaa catagtccgc gcttccgtgg
      481 cctggcgctc ttgccgcagg ctttgatcaa tcctcaggcg gcggccgatg agcttcgccg
      541 cgccgtaaag cagctcggca tgtgcggcgc ggtgatgccg tcgaacagtt tgcacgcgcc
      601 gccgttgggt acggcgacat accttccggt ttatcaagtc gccaatgaat tgggttgctg
      661 cgtcgccatc catggcggcg tgcacggcca catgggtatg gacggcctcg acccctatgc
      721 ggcggtacac gccatcggcc atccgctggg acagatgatc tcgctggcgt cgctggtctt
      781 caacggcgtg ctggataggt tcccgaagat tcgtttcggc tttctcgaag gcggcgttgc
      841 ttggttactt ttctgtctgg agcgtttcga tcgatcctac gatagtcatg tgatgcgcga
      901 tctgcgcggc agttatctaa aactacagcc cgctgagaaa atcagcgact acatcatccg
      961 ccatgtcaaa gcacggcgca tcttcgtcgg ctgcgaaggc ggcgaaccgc tgcttgccac
     1021 cgctgtgaag atggtcggca acgatccgtt catgtattcc agcgactttc cgcacgaggt
     1081 caacgccgcg acgtgcaaaa aagagttggc agagttggtc gagaatcccg aactgtcggc
     1141 aacggataaa ggcgcgatct tgtatcagaa tgcgatccaa ttttacggca taagggaaaa
     1201 ttaaccgcag ccttcgacga tgctcagggc gatcggggca taagtgtgac gggcgatgac
     1261 cgtcatcccc ggtgacacga gggatctcag caagatttct cccaagggtc gaaatgacaa
     1321 cgactacgaa ctcggcgtct ttgcgccttg gcgggagtaa cgttcctcaa ttcggcatga
     1381 agacgagaaa atattggtgc ggcaggaatt tgtgttcttt gctgagtcgg aagccgttgg
     1441 tttccatctg tttgatcaaa tcctcgcggg ctattttcat ttgcaatggc gggccgaagg
     1501 ggagctcttt tttgtggaag tcgatcatga cgatctcgcc gccgggtttg agcatcttct
     1561 tcatcagcgt gagatattta gtttgattct cgacatggtg ccagacatcg cagatgaaaa
     1621 agcgatcaac cgatcggtcc ggcagcagcg gatcatccag atcggcgagg atggagacga
     1681 cgttggccga tttggtttcg cggatgcgcc ggttgatgtg gcggatcatg tcggcgctga
     1741 cgtctacggc gtagactttg cctttgtcgc cgacgtgatg ggctaagggg aacgtgaaat
     1801 agcccgtgcc ggcgccgata tcggcgatgg tttcgccggg cttgatgttg agcgccttca
     1861 agacctcatg aggtttctga tattcatcgc gcttgggatc ctccaaagct ttcatatagg
     1921 ctttgggatc gttgtgcagg ccatgcatct gttgctgatt gcgtttggcg gcgtcttggg
     1981 cgtagagggt cgtcgatatg aacccgagaa ataggagcga taaagatagt aatgattgga
     2041 tcatgacagc gtgacctcgt tgaattagtc ctagttagtc gggtcgcacc gatcactgtt
     2101 tcacttgcgt tgagcgggtt agcctgtttt cgaataatat cattccatgc ttgcaagtct
     2161 atcggcgggt acgggttgga agactcgcgg attagagtgt aaagtgcagc tgcagtgcga
     2221 ccgtgagagg aggtgcaagc aatgggcgag ctagttgatt atcagatcgc aattgaccgt
     2281 cggcgcaata agcgcggcag ttggaagcat gctcgggaac gtatgctcaa tgaggtctgg
     2341 ccggtgatca cgccgtcttt cgagctgaca cccggtggca agatttttac catcggtagc
     2401 tgcttcgccc gcaatataga agaacatatg caacggctgg gtttcgagat accgatgtta
     2461 aagttccggg tgcccgcgga ggaatggggc gcgagagcta acggcatcct caacaaatat
     2521 accccggccg ccattttcca agaaatcgac tggaccaaaa acataatggt cagagacggc
     2581 gtcgtgcgcg aaagcgattg tgcagccttc cgcttcgact gtgatggtga tttttgcgtc
     2641 gatacgaact tagccggttt caatttggtt tcacgccagc gttacttcga gaggcggtgc
     2701 cagatatatg aagtattcaa agaggtgttt accgctgact gtgtggtgat taccttggga
     2761 ttggtcgaag cttggctcga tcgcgagaag ggcatctaca tccaagaagt acccagcggc
     2821 aagatttttg ccaagcaagc tcgacggttt gcgttcgagt cgctaagcta cgaaaaatgc
     2881 cgcgattaca ttcagcgatc ggttgatacc gtccgtgagg taaacaagaa cgccaagatt
     2941 ttaattacca cctcaccggt gcctcttagc cgaaccttta ccaccgacga tgtcatcata
     3001 gccaattcgc attccaagtc ggtgctccgg gcggtcgccg gcgagattgc cgccgccaat
     3061 gtcgccatgg actatttccc gagttacgaa agcgttagcg gcaccaagag ctggcagatc
     3121 tggggcgaag atttgctcca cgtctcggat gcgttcgtcg gcaaggtcgt ttcccggttg
     3181 accgacatat attgtcccgg catccagcag tcgcgcaaga agtacctgga cttttacatg
     3241 gcttctaaag gagacgatct cagtgaggca ttggagttgg gtgaacagct aagcgaaact
     3301 aactccgtcg aatacctcga aagattaagc caggtattag agcgaaaggg cgatatcaac
     3361 gcagcagtgc atgagctcaa gaaagccctc gaccaagttc cggctgaacc caggctcagg
     3421 tttcaataca gcaaactttt ggcgcaaagc gggcggatgc aggatgctct gtccgaagct
     3481 cgttatggtg tggcattgga tccaacaaat ctgcgttatc gccgccacct ggtcactctc
     3541 tacgcgcgcg atcgaaacta cgtcaaagca cttggccaac gcgtgttggc ggcactctat
     3601 cgccgccagt ctaaaactca aaacgtaacc ctaaagcgtt tgctgcagct cgtgtgccat
     3661 caaggcatca ccgcgcaaaa atcctgacgc gggttgctgc cggacgatgc tgttacctgc
     3721 cggtgaccgg cctaagatcc atcgatttaa ctcagcaccg gacgaacatc cgtgctcggc
     3781 ctgcatctgt tctgctgtag ttcccccttg aaggtttctc gctcgggaaa tcacatttcc
     3841 aagttgcctg ttttgcggtt tccctgtatt tctaatcgct atggcgcaaa ccgaactcaa
     3901 gcggcaaata ggattgtttt cagcggtgat gttgattgcc ggcgatatga tcggcacggg
     3961 aatttttatt tctaccggcg cgatagcgga gaccttgccg tcacccggcg gggtcttgct
     4021 cgtctggctg tttggcggtt tgctcgccct tgccggcgcg ctgacctgcg cggaattggc
     4081 cgcaagcctg ccttacgccg gcggcgacta tgtttacatc cgcgaagcct acggcaggct
     4141 aatgggattt ctctcgggct ggtcttcgtt tctcgtgact ttttccggtg ccatcgcttt
     4201 tctcgcggtc atcttgaacg gctttgtgtc attctttatt cccgtctt
//
LOCUS       DASQKS010000259         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_990054, whole genome shotgun sequence.
ACCESSION   DASQKS010000259 DASQKS010000000
VERSION     DASQKS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990054"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VE170_06740"
     CDS             <1..371
                     /locus_tag="VE170_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15169.1"
                     /translation="GKPETAAQYTAKYDAFLSTLRGAAPTKPILCVTPILNRADLDRG
                     GNRNGERPELYREGITRVIRQRQNSDTNLHLSDGLTMVNDPLFLLVTDRVHPNDAGMH
                     RIANGVAQALKPLLANLRAS"
     gene            complement(455..790)
                     /locus_tag="VE170_06745"
     CDS             complement(455..790)
                     /locus_tag="VE170_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15170.1"
                     /translation="MKVGQATAGESRSCPHCRATILKSAATCPMCRHNLRFVSIGAEP
                     RAKPISCPLWVEGTLQNASETETLEYYLLMEVRDEAGKVISRQSVGVGAIPQSAKRIF
                     SLRIETTPA"
     gene            complement(787..1326)
                     /locus_tag="VE170_06750"
     CDS             complement(787..1326)
                     /locus_tag="VE170_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ15171.1"
                     /translation="MALWNQATPEVSAEKPQQVPPPLTPTPTATATANVPTPIPVAPA
                     AKESAPSGRRESVFGPGVTIEGKIEGDADIRIGGKFKGDIQIKGDLNIDKGAKVSAKV
                     QAANVTIGGELEGNVVAAAQVKLLETGQLIGDLKAASLTVVAGSRMRGNVEFGWSASE
                     VAKFANGHAHDQEKVGAAG"
     gene            1826..>2580
                     /locus_tag="VE170_06755"
     CDS             1826..>2580
                     /locus_tag="VE170_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15172.1"
                     /translation="MKAEQPSDAAREAFHQRMHANNMYGLWELASQMTPQPQPKMIAH
                     MWPWSTLERIVSESTQVVPVGDERRALQLFNPGLNGRWATTNNLIAAVQVLLPGEVAR
                     AHRHTPTAIRFILEGSGAYTAVDGERVYMEPGDLILTPSWSWHGHGNETKDRIVWMDG
                     LDIPLVASLEAMFFQFYKETQVPASRPAHTSQAMYGTGQLNPTWVKESSVASPLLLYS
                     WEQTRQALDALRGQTGSPFDGISLNYRHPLTGGS"
BASE COUNT          567 a          781 c          701 g          531 t
ORIGIN      
        1 acggcaaacc ggagaccgcg gcgcaataca ccgccaagta tgacgcattt ttatcgaccc
       61 taagaggtgc cgcgccgacc aaaccgatac tctgcgtgac accgatcctg aaccgggcgg
      121 atctagatcg cggcggcaat cgtaacggcg agcgtcccga gctttatcgt gaaggcatca
      181 cccgcgtcat acgccagcgg cagaacagcg atacgaactt acatttgtca gacggcctga
      241 cgatggtcaa cgatccgctc ttcctgctgg tcaccgaccg cgtccacccc aatgacgccg
      301 gcatgcaccg tatcgctaac ggcgtagccc aagcgctaaa accgttgtta gcgaatctgc
      361 gcgctagtta gcagctaaaa aaaaatgggc ccgagacgcg cgcgctggac agcgtacgtc
      421 tggggcccat ggcctactca ggataaaatt gcgctcaggc cggtgtggtc tcgatacgca
      481 gagaaaagat tcttttcgcc gactgcggga tcgcgccaac gccgacgctt tgccgcgaga
      541 tcaccttacc ggcctcatca cgcacttcca tcagaagata atattcaagc gtttcggttt
      601 cgctggcatt ttgcaaagtc ccttcgaccc acaacgggca gctgattggc ttggcccgcg
      661 gctcggctcc gattgagacg aagcgcaagt tatggcgaca catcgggcag gtcgccgcgc
      721 ttttaaggat cgtcgctctg cagtggggac agctgcgcga ttcgccggct gtcgcctgcc
      781 ccactttcat ccggcggctc cgactttttc ttgatcatgc gcatggccgt tggcgaactt
      841 ggcgacttcg ctggcgctcc aaccaaactc gacattaccg cgcatacgcg agcccgccac
      901 caccgttagc gaggctgctt tgaggtcgcc gatgagctgg ccggtctcca gcaattttac
      961 ttgggcggcg gcgaccacgt tgccttcgag ttcgccaccg atggtgacat tggcggcctg
     1021 caccttggct gaaaccttgg cgcctttgtc gatattgaga tcgcccttga tttggatgtc
     1081 gcccttaaac ttgccaccga tgcgaatatc tgcgtcgcct tcgatcttgc cttcgatggt
     1141 aacaccgggg ccaaataccg attcgcggcg gcccgatggt gcgctttcct tagctgccgg
     1201 cgccaccggt atcggtgtcg gtacgttcgc ggtcgcggtc gcggtcggag tcggtgttaa
     1261 aggtggcggc acctgctgcg gtttttccgc actcacctcg ggcgtagctt gattccacaa
     1321 agccatgaga cctccttgtc atgaaaacta ttttcggatg gaaacgcggg cattttcgac
     1381 aaacggatag tttttgagca agggaagtgc cagagaccaa ataagcggtt agaatctcta
     1441 gccaaatttt tcacggggca ctacccgaca aaggcttggg tcaaaccagc cggaaatagt
     1501 ccgatctgcc aataaatgcc gcatggcaaa accaccccgt caaaaaatcg acgacttaat
     1561 cgacaattaa tcaacggttg tctgcgaata gcttcacaag caatgcaggg aatagcagtc
     1621 ggttaatgaa cccaaactgt cgcttcaaat cggaaagaac tgctcggcga aaaactctat
     1681 cacttgaccg acgtcagtaa ccgtaatagc gttcactagt gttttaccta tgcccggtaa
     1741 agcgtttacc ctatgctcgt tacgatcact cgcgcgcgaa acgagagtcg attccgagcc
     1801 ggaacatttc aacgaaggac gaatcatgaa agcagaacag ccatccgacg ccgcgcgcga
     1861 agccttccac cagcgcatgc acgccaacaa catgtacggt ctgtgggagc ttgccagtca
     1921 aatgaccccc cagcctcagc ccaaaatgat cgcccacatg tggccttggt cgaccttgga
     1981 gcggatcgtc agcgaatcga ctcaagtcgt gccggtgggt gacgagcggc gcgccttaca
     2041 gctattcaat ccaggactca acggccgctg ggcgaccacg aacaatttga tcgctgccgt
     2101 gcaggtgctg ctccccggcg aagtagcgcg cgcgcaccgc cacacaccga ctgcgattcg
     2161 ctttatctta gaaggcagcg gcgcctacac cgccgttgac ggcgaacgcg tttacatgga
     2221 gccgggcgat ttgattctga cgccaagctg gtcctggcac ggtcatggca acgaaaccaa
     2281 agaccgtatc gtctggatgg atggcctcga catcccgttg gtggcgtcgc tggaggcgat
     2341 gttttttcag ttttacaagg agactcaagt gccggcgtcg cggccggcgc acacttccca
     2401 ggctatgtac ggcaccggcc agctcaatcc tacctgggtc aaagaatcgt ccgttgcctc
     2461 gccgctgttg ctctactcct gggaacaaac ccggcaagcg ctggacgccc tgcgcggtca
     2521 gaccggcagc ccattcgacg gcatctcgct aaactatcgc catccactga ccggcgggtc
//
LOCUS       DASQKS010000260         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5591229, whole genome shotgun sequence.
ACCESSION   DASQKS010000260 DASQKS010000000
VERSION     DASQKS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5591229"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VE170_06760"
     CDS             <1..184
                     /locus_tag="VE170_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ15173.1"
                     /translation="NGRWPEYETALKANGIKYEAHVYPGTNHGFHNDTTPRFNEAAAK
                     LAWQRTLDHFNKNLKA"
     gene            486..1163
                     /locus_tag="VE170_06765"
     CDS             486..1163
                     /locus_tag="VE170_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HYJ15174.1"
                     /translation="MFEHQIKNSDDSTDTERRVLEAAGEVFAALGYRAATVRQICEKA
                     GANIAAVNYYYGDKERLYQAVLRSVPDAQAIKYPSRSGLSPDATAEERLRVYVHSLLH
                     RIFDAGRPGWHSKIIAREMIEPTRALDSLLEEVALPLHRELASIVRLLLGASATDEDV
                     RFCALSIMGQCVYYHHARAVLVRLYPEQQYRREDVARMVEHISEFSLAALRELAQRRQ
                     AQGNETP"
     gene            1150..2373
                     /locus_tag="VE170_06770"
     CDS             1150..2373
                     /locus_tag="VE170_06770"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ15175.1"
                     /translation="MKLLNLKSLTNRRALAGLFVIVALGAAACGGDSPATAVPKSDTP
                     SRQVRVVAAAQSVAARVVSASGTLAADDQFVLGTKVIGRLGEITVDLGSRVKKGQALA
                     RIDQSDYRLRVEQADAALQQSRVRLGLPASGSSDQVDIEQTSLVRQGAAVLKEARLNH
                     DRMVELWDRKLIARAELDSAVSQLAVAEGRYQDAIEEVRNRLAVLVQRRSELEIARQQ
                     LADTVIVSPIDGAVSERQAAVGQYLPAGAPVVTLVRTDPLRLRLAVSEREAGLVRVGQ
                     TVNLTVESDANKYQGRVARLAPAIAENNRTLLIEAQVPNGQGALRPGSFAKADIVIEA
                     GQEIITVPRNSIITFAGIEKVLMVAQGKTIEKRVRTGRRIGEQVEVTEGIVAGNQVIV
                     QPGNLVAGEPVTVVN"
     gene            2373..>3305
                     /locus_tag="VE170_06775"
     CDS             2373..>3305
                     /locus_tag="VE170_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ15176.1"
                     /translation="MQKLAEVSIRRPVFASMIILALVVVGVASYFRLGVDRFPSVDLP
                     TVSVRTTLPGASPEEVETIISQPIEEVVNTVEGIDELRSVSGRGTSFVMATFNLDRDI
                     DVAAQDVRDRVATVLNRLPNDVDPPVISKFNNEDSPILTIAVSGDRSLRELTEIADKI
                     VKVQLERSVGVGEVRIVGGLERAINIWIDADRLAAYQLPITAVRDAIQRQNADIPGGN
                     VTSGAREQVLRTMGRITDPKAFDDLVISTVNGSPIRVRDIGRAEDGTKEQRSLSRLNG
                     VPTVQLQVRRQSGANTIAVIESVKENLARVARQLP"
BASE COUNT          713 a         1001 c          948 g          643 t
ORIGIN      
        1 caacggcagg tggccggaat acgaaaccgc cctcaaagcg aatggcatca agtacgaggc
       61 ccacgtttat cccgggacga atcacggctt tcacaacgac accacgccac gcttcaatga
      121 agcggccgcc aaactcgcct ggcagcgcac cctcgatcac ttcaacaaaa atttgaaagc
      181 gtaaaaatcc aaattggcag gtaggggcga aatattcgcc cctacctgcc acatcgtcct
      241 tcgttttcga tgacttttta aagtgcgcgc cgataactag gtagttgtcc ttccctcggc
      301 gtggagtgcc ccccgattct aaaatagtgc tgcccccgag cacctggtaa cttacgcgat
      361 cgccgttgct ggtgcgctta ttacctcttg accactattt ttagttttag tttacagata
      421 cttaaaaaag aaattcattt ccaattgtta ttcatacgat tgtttcatac aattgtttca
      481 aatatatgtt tgaacaccaa atcaaaaatt ctgacgacag tactgatacc gagaggcgag
      541 tcctggaggc tgctggggaa gtttttgctg ccctcggcta tcgcgccgcc actgtgcgcc
      601 agatctgcga aaaagccggg gcaaacatcg ccgcggtcaa ttattactac ggcgataaag
      661 agcgactcta tcaggccgtg ctgcgttcgg tgccggatgc ccaagcgata aaatatccat
      721 cgcggtccgg actgagcccc gatgccacgg cggaggaacg actgcgcgtc tacgttcact
      781 cccttttgca tcgcatcttc gatgccggcc gtcccggatg gcacagcaag atcatcgccc
      841 gcgagatgat cgagcccacc cgcgccctcg attcgctgct cgaagaagtc gcgctgccgc
      901 tgcatcgtga attggcgagc atcgtgcgct tgctgctcgg cgccagcgcc acagacgaag
      961 acgtgagatt ttgcgcgctc agcatcatgg gccagtgcgt ttactaccat catgcccgcg
     1021 cagtcctcgt gcgcctctac ccggaacaac agtaccgccg tgaggatgtc gcgcgtatgg
     1081 tggagcacat cagtgaattt tcattggcgg cgctgcgaga gttagcgcag cgaaggcagg
     1141 cccaaggcaa tgaaactcct taatctcaaa tcattgacaa accggcgggc actagcggga
     1201 ctgttcgtta tcgttgcgct gggcgccgcc gcctgcggcg gcgacagtcc agcaaccgcg
     1261 gtgcccaaaa gcgacacgcc gtcgcgtcag gtaagagtgg tcgcagcggc gcaatctgta
     1321 gccgcgcgcg tcgtcagcgc cagcggcaca ttggcggccg acgaccaatt tgttttgggc
     1381 acgaaagtca tcggacgcct gggcgagatc actgtcgacc tcggcagccg cgtgaagaag
     1441 ggtcaagcgc tcgcgcgcat cgaccaaagc gactatcgcc tgcgcgtcga acaagctgat
     1501 gccgcgctgc agcaatcgcg ggtcaggctg ggactacctg cgtcgggaag tagcgatcag
     1561 gtggatatcg agcaaacatc gctagtccgc caaggtgcgg cggtattaaa agaagcccgg
     1621 ctaaatcacg accggatggt cgaactttgg gatcgcaagc taatcgcgcg cgccgagctc
     1681 gacagcgccg tatcccagct tgcagtggcg gaagggcgct atcaagacgc catcgaagaa
     1741 gtccgcaatc gcctggcggt gttggtgcag cgccgttccg agctcgagat cgcgcgccaa
     1801 cagcttgccg ataccgtcat cgtttcgccc atcgacggcg ccgtcagcga acgccaggcg
     1861 gccgtcggcc agtacctgcc ggccggcgcg cccgtcgtga ctctggtgcg tactgatccg
     1921 ttgcgactcc gcctggcagt ttccgagcgc gaagccggct tggtccgcgt cggccagacc
     1981 gtgaatctca ccgtcgaaag cgatgccaac aaatatcagg gccgggtcgc ccgcctcgcg
     2041 ccggccatcg ccgagaacaa ccgaactttg ttgatcgaag cgcaagtgcc caacggccag
     2101 ggcgcactgc gccccggttc gtttgccaag gcggacatag ttatcgaagc cggtcaggag
     2161 atcatcaccg tgccgcgcaa ctcgatcata acttttgccg ggatcgagaa ggtcctcatg
     2221 gtggcgcagg gcaaaactat tgagaagcgc gtgcgcaccg gacgccggat cggcgaacag
     2281 gttgaagtta ccgaaggtat cgttgccggc aatcaagtca tcgtgcagcc gggcaacctc
     2341 gtcgccggcg agcccgtgac cgtggtgaac tgatgcaaaa gctggcggaa gtttccatta
     2401 gacgaccggt ttttgcctcg atgatcattc tggcgctggt ggtcgtcggc gtagccagtt
     2461 atttccgtct cggcgtagac cgctttccat ccgtcgacct gcccaccgtc tcggtccgca
     2521 ccactttgcc cggcgcctcc cctgaggaag tcgagacgat catttcacag ccgattgaag
     2581 aggtggtcaa cacagtcgaa ggaatcgacg agctccgttc cgtatccggc cggggaacct
     2641 cgttcgtgat ggcgacgttc aacctcgacc gggatatcga cgtcgccgct caagacgtgc
     2701 gtgatcgagt cgctaccgtg ctcaatcgtt tgccgaatga tgtcgatcct ccggtaattt
     2761 ccaagttcaa caacgaagac tcgccgattc tcaccatagc cgtttccggc gatcgatcgc
     2821 tgcgcgagtt aactgagatc gccgataaaa tcgtcaaggt gcagctcgaa cgctcggtcg
     2881 gcgtaggcga ggtacgcatc gtcggcggtt tggaacgtgc catcaacatc tggatagacg
     2941 ccgaccgctt agcggcctac cagctgccaa tcacagcggt tcgcgacgcg atccaacgcc
     3001 agaatgccga tatacccggc ggcaatgtga ccagcggcgc gcgcgaacag gtcctgcgta
     3061 ccatgggaag aatcacggat cccaaggcgt tcgacgacct cgtcatcagt accgtgaacg
     3121 ggtcacctat tcgggtccgg gacatcggtc gcgccgaaga cggcaccaag gaacagcgct
     3181 ctctgtcgcg attgaacggc gtgccgacgg tccagcttca ggtgcggcgc caatcaggcg
     3241 cgaacacgat tgcggtcatc gagtccgtaa aagaaaacct cgcgcgcgtc gcccggcagc
     3301 ttcca
//
LOCUS       DASQKS010000261         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1391419, whole genome shotgun sequence.
ACCESSION   DASQKS010000261 DASQKS010000000
VERSION     DASQKS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391419"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VE170_06780"
     CDS             complement(<1..1104)
                     /locus_tag="VE170_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ15177.1"
                     /translation="MNPISSPYGSWKSPITANLIGGGEIGLEQIRIDGQDIYWIERRA
                     QEGGRKVIVRRAANGEITDVTPVGFNARTRVHEYGGGDYAVADRNIVFSNFTDQRLYL
                     QAVDAQPRPLTSSASRRFADGQFDRRRNLFYTVREDHTGSGEAVNTVVALDLDSDDGG
                     TLVLSGNDFYTSPRLSPDGSQLAWLSWNHPNMPWDGTELWLAQLNGDGSVGVRQLVAG
                     GDNESIYQPQWSPDGVLHFVSDRTGWWNLYRWRDNQVEALCPMAAEFGQPQWAFGGSL
                     YGFVSERQIVCSYTKNGTDYLAALDGETKTLRAIELPASAISQVRVAADRVVFIGASA
                     TETSAVIALDLSTGKLQVLRRSRETGVDAGYLSE"
     gene            complement(1141..2931)
                     /locus_tag="VE170_06785"
     CDS             complement(1141..2931)
                     /locus_tag="VE170_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYJ15178.1"
                     /translation="MLRGIGYTLGAVLLLLLLQASALSQVPPANDPAKALHALFAEDW
                     DYRMEQHPTWASGLGDRRWNHRWSDIGLDAIFKRHDHDITTLAKLTKIDRGALTPADQ
                     LNYDLFKKDYEDDVQGFQYRWYLLPLNQLGGVHTVNQLVNVLRFQTLKDYDDWIARLR
                     ALPTLVDQTIALLRLGLKKGIIHPQIVLQRVPAQIDHHIVGDPKASPLYTPFTKFSSS
                     ITPTDRASLTQAAEDAIATAVVPAYRKLKFFIVNDYLPAAPDRVGIWQFPSGAAMYAH
                     ATRTNTSTDLAPQRIHEIGLEEVKRIRREMQAIIDKLGFKGSFAEFAKFLRNDAKFYF
                     TSENELLEAYRALSRRIDPLLVKVFRVLPRMPYGIEPVPANIAPDSPVGLYRGPAADG
                     SRAGIFSINTFKPEIRPKYEMMALSLHKGVPGHHLQIALAMEQQGIPNFRRYGGYTAF
                     IEGWGLYAESLGDETGLYDDPYSKFGQLMYEIWRASRLVVDTGMHHLRWDRQKAIDFM
                     AENTVKQELEVAAEIDRYIVWPGQALAYKIGQLKISELRAKAQQTLGDKFDIRDFHDE
                     LLRDGAMPLDRLETKINSWLAKHQQAETQK"
     gene            complement(2967..4097)
                     /locus_tag="VE170_06790"
     CDS             complement(2967..4097)
                     /locus_tag="VE170_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15179.1"
                     /translation="MADRRQDMTRRDFLKLAGKSAVMSSGLLAGCAATATTAPSTSRA
                     IDIHHHYFAPELIDEIRKHGKALGGIEHFPPKQAKDNPYRIQFPKGRAFAPDLRMAEV
                     PNRLNAMTKGNVGIAMVEVHTASVGYELDGAHGESWSNIYNEAIMRLVKKHPERFAGI
                     ATVPLQDPPRAAQVLENAIVNLKMSGVTIASNVVGKYFNDKSFDPFWKKAEELDVLMI
                     MHPEWVAGAEKMDPYSLRNICGNPADTTLSVAYMMYSGVFDRFPNLKLGLLHGGGYIP
                     YHLGRLDQGKIGASGSAAIPAKLPPSKYLKNLYFDNLVYRVETVEYLKRMVGADHIMV
                     GTDYPFDLGDWWAAEKIQQMECTGAEREAMLHGNARRLLRIG"
     gene            complement(4103..4840)
                     /locus_tag="VE170_06795"
     CDS             complement(4103..4840)
                     /locus_tag="VE170_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15180.1"
                     /translation="MQRHRSRSSSLEVPPGAGVSRDHKVILFDYRGTGKSSKTVEKYS
                     IKMFTDDAAAILAHLNVAQAIVCGHSMGGVVAQLLALDYPGKVKKLILASSGSAHPGA
                     HGIPLAMCRDMVRDGFEKYIREHTIDTGWTKEFVAKNPELIEKFLQVRMSGIAPLENY
                     LHFVLARQSHDHTDRLKEITAPTLVFVGDDENHGATDNSHWAAAHQLAKAIPNAKLVV
                     LPGEGHHYLATNPAAAHKAIRDFIKQG"
BASE COUNT          942 a         1347 c         1502 g         1149 t
ORIGIN      
        1 ttcggagaga taaccggcgt cgacccccgt ctcccgtgat ctgcgtagga cttggagttt
       61 gccggtcgat aggtccaatg cgatgaccgc gcttgtctcc gtcgccgacg caccgatgaa
      121 aaccacgcgg tcagcggcga cccgcacttg ggaaatcgcc gaagcgggaa gttcgatggc
      181 gcgcagtgtt ttggtttcgc cgtcgagcgc ggcgagatag tcggtgccgt tcttggtgta
      241 gctacagaca atttgtcgtt cggaaacgaa gccatagagc gagccgccga atgcccattg
      301 cggctggccg aactccgctg ccatcgggca cagcgcttca acttggttat cgcgccagcg
      361 gtaaagattc caccatccgg tgcggtcaga aacaaagtgc agcacgccat cgggagacca
      421 ctggggctgg taaatggatt cgttatcgcc accggcaacg agttggcgca cgcccaccga
      481 tccgtcaccg tttagctgag cgagccaaag ttcagtgcca tcccacggca tgttgggatg
      541 attccagctg agccaggcta gttgggaacc gtctggactg agacggggcg acgtatagaa
      601 gtcattccct gatagtacca gggtgccgcc gtcgtcgctg tcgagatcga gggcgacgac
      661 agtattgact gcttcgcccg agcccgtgtg gtcctcgcgg acggtgtaaa aaagatttct
      721 ccgccgatcg aattgtccgt cggcgaaacg tcgtgatgcg ctggaagtta gcggtcgcgg
      781 ttgcgcgtca acggcctgca ggtacagccg ttgatcggtg aaattcgaga agacaatatt
      841 ccggtcggcg accgcataat cgccgccgcc atactcgtgc acgcgggtgc gggcgttgaa
      901 tcccaccggc gtgacatcgg tgatttctcc atttgccgcg cgccgcacga taactttgcg
      961 cccgccttcc tgggcgcgtc gctcgatcca atagatgtcc tggccgtcga tgcgaatttg
     1021 ttccaggccg atttcgccgc cgccaatcaa attggctgtg attggcgact tccacgagcc
     1081 gtaaggggag ctgattggat tcataaaatg gttcgtttca tttattccag caattttttg
     1141 ctacttttgt gtttccgcct gttggtgttt tgccagccac gagttgattt tagtttccaa
     1201 ccggtccaac ggcattgcgc cgtctctgag cagttcgtca tggaagtcgc ggatgtcgaa
     1261 tttgtcgcca agagtttgct gcgccttggc gcggagctcg gagatcttga gctggccgat
     1321 cttgtaagcg agggcttggc ccggccagac gatatagcga tcgatttcag cagccacttc
     1381 cagttcttgc ttcacggtat tttccgccat gaaatcgatg gctttctgcc ggtcccaacg
     1441 cagatggtgc atgcccgtgt cgaccactaa ccggctggca cgccagattt cgtacatgag
     1501 ctggccgaac tttgagtagg gatcgtcgta caagcccgtc tcatcgccaa gcgattcggc
     1561 gtagagtccc catccttcga tgaacgcggt gtagccgccg taacggcgaa agtttgggat
     1621 gccttgctgc tccatggcga gagcgatctg caggtgatgt ccgggcacgc ccttgtgcag
     1681 cgatagcgcc atcatttcgt acttcggtct gatctccggt ttgaaagtat tgatgctgaa
     1741 tatgccggca cgggagccgt cggcagccgg accgcggtag agcccgaccg ggctatcggg
     1801 tgcgatgttt gccggcaccg gctcgatgcc gtatggcatg cgcggcagga cgcgaaaaac
     1861 ttttaccagt aacggatcga tgcgccggct caaggcgcgg tatgcttcga gtaactcgtt
     1921 ttcacttgtg aaataaaatt ttgcatcgtt gcgcagaaac ttggcaaact cggcgaacga
     1981 acctttgaag cctaatttgt cgatgatcgc ctgcatctcc cggcggatgc gtttgacctc
     2041 ttctaaaccg atttcgtgaa tcctctgggg cgcgagatcg gtgctggtat tggtgcgcgt
     2101 ggcgtgggcg tacatcgccg cgccgctggg gaactgccag atgccgaccc gatccggcgc
     2161 tgcaggcagg tagtcgttga cgatgaagaa tttgagctta cgataagctg gaaccacggc
     2221 agtggcgatg gcatcctcgg cagcctgggt gaggctcgct cggtccgttg gcgttatcga
     2281 cgatgagaat ttcgtgaagg gtgtgtagag cgggctcgct ttcggatccc caacgatatg
     2341 atgatcgatc tgcgctggca ctcgctgcaa gacgatctgc ggatgaatga tacctttctt
     2401 gagccccaga cgtagaaggg cgatggtctg gtcgacaagt gtgggcagcg ctcgtagtct
     2461 ggcgatccag tcgtcatagt ccttcagggt ttggaaacgc agaacgttga cgagctgatt
     2521 gaccgtgtgc acgccgccca actgattgag cggcagcaga taccagcggt attgaaaccc
     2581 ttggacgtcg tcttcgtaat cttttttgaa caagtcgtag ttcagttgat ctgccggcgt
     2641 gagtgcgccg cgatcgatct tcgtcagttt ggccagcgtc gtgatatcgt gatcatgccg
     2701 tttaaatatc gcgtctaggc cgatatcgct ccagcggtga ttccaacgcc ggtcgcccaa
     2761 gccggatgcc caggtcggat gttgctccat ccggtagtcc cagtcctcgg cgaagagcgc
     2821 gtgcagggcc ttggccgggt cgttcgcggg cggcacttgc gacaaagcgc tcgcctgaag
     2881 caacaacagc aacagcactg cgcctaaggt atatccgatt ccccgcaaca ttaggtgcga
     2941 ctcactcgat tgggtttttt cagaagctaa ccaattctta gtagcctccg cgcgttgcca
     3001 tggagcatgg cttcgcgttc ggcgccggtg cattccattt gctgaatctt ctccgcggcc
     3061 caccaatcgc cgagatcgaa cgggtaatct gtgccgacca tgatgtgatc ggcgccgacc
     3121 atgcgtttca agtattcgac ggtctcgacg cgatagacta ggttgtcgaa gtagagattt
     3181 ttcagatatt tgctcggtgg caacttcgct gggatcgcgg ctgagccgga agcgccgatc
     3241 ttgccttggt ccaatcggcc caggtggtag gggatatagc cgccgccgtg gagtaagccg
     3301 agtttcagat tgggaaagcg gtcgaagacg ccgctgtaca tcatgtaggc gaccgacaac
     3361 gtcgtgtccg ccggattgcc gcaaatgtta cgcagactgt atgggtccat tttttcggcg
     3421 ccggcgaccc attccggatg catgatcatg agcacgtcga gctcttcggc ttttttccag
     3481 aaaggatcga agcttttgtc gttgaaatat ttaccgacga cgttggaggc gatggtgacg
     3541 ccggacatct tgagattgac gatggcgttt tccaacactt gggccgcgcg gggcggatcc
     3601 tgcaacggca cggtggcgat gccggcaaag cgctcgggat gttttttgac caatctcatg
     3661 atcgcttcgt tgtagatgtt cgaccagctc tcgccgtgcg cgccgtcgag ctcgtagccc
     3721 accgacgcgg tgtggacttc gaccatggcg atgccgacat tgcctttggt catggcgttg
     3781 agacgattgg gcacttcagc catccgcaaa tccggggcaa acgcccggcc tttggggaat
     3841 tgaatgcgat agggattatc tttggcttgc ttgggcggaa aatgctcgat gccgcctaag
     3901 gctttgccat gtttcctgat ctcgtcgatg agttccggcg cgaagtaatg gtggtgtatg
     3961 tctatggcgc ggctcgtgct aggagccgtc gttgctgtcg cggcgcaccc cgcgagcagg
     4021 cccgatgaca tgaccgcgct cttgccggcg agcttgagaa agtcacggcg agtcatgtcc
     4081 tgtcggcgat cggccatctt cactacccct gcttgataaa atcgcggatt gctttgtgcg
     4141 cggctgccgg gttggtcgcc aagtagtgat gcccttcgcc gggtaacacg acaagtttgg
     4201 cgttaggaat cgccttcgcc agttgatgcg ccgcggccca gtggctgttg tcggtggcgc
     4261 cgtggttttc gtcgtcgccg acaaagacca gagtcggcgc ggtgatttcc ttcaatcggt
     4321 ccgtgtggtc atggctctgg cgcgccagga cgaagtggag ataattttcc agcggtgcga
     4381 tgccggacat cctcacttgg agaaactttt cgatcagctc gggattcttg gcgacgaact
     4441 ctttggtcca gccggtgtcg atggtgtgct cgcggatata tttttcaaat ccatcgcgca
     4501 ccatgtcgcg gcacatcgcc agtgggatgc cgtgggcgcc cgggtgcgcc gagccgctgg
     4561 aggcgaggat cagttttttc accttgcccg gatagtcgag cgccaacagc tgggcgacca
     4621 cgccgcccat ggaatggccg cagacgatcg cttgcgcgac attcaaatga gcgaggatcg
     4681 ccgcggcatc gtcagtgaac attttaatcg aatatttctc gaccgtcttg ctcgacttgc
     4741 cggtgccgcg gtagtcgaac aggatgactt tgtgatcgcg ggaaactccg gcacctggtg
     4801 gaacttccag gcttgatgat cgagaccggt gacgctgcaa aaaagcaacg ccgggccgtc
     4861 accgtgagtt tcgtaataaa tctcgctgcc gtcgcgatcg agaaatggca tggggctcct
     4921 agtcgctaaa ttcgcccgcg
//
LOCUS       DASQKS010000262         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2239018, whole genome shotgun sequence.
ACCESSION   DASQKS010000262 DASQKS010000000
VERSION     DASQKS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239018"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VE170_06800"
     CDS             <1..732
                     /locus_tag="VE170_06800"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15181.1"
                     /translation="QGSYLKILSSISSTLTHTLIAHPSIKRAEELRGKRFGIQNPGGT
                     TWINTILALEHVGLEPKRDNINLLPIGDSVLVGQALEAGRVDAAVLDGALVRRLRSKG
                     FAIIAELQPAKIPLLSQAIVVAPEFLQKRGDITEKLLMTLVESLAYSMAPMNKAVVIK
                     TMMRKFQIADPAVAEEGYHDYLNGVERRPIPTPDGMRNIRRLMATLNPKIASVKVEDL
                     IDSRLIRKLDENGFIDKMAVNYGLK"
     gene            811..1482
                     /gene="tenA"
                     /locus_tag="VE170_06805"
     CDS             811..1482
                     /gene="tenA"
                     /locus_tag="VE170_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HYJ15182.1"
                     /translation="MAKRFSAELRQQSESIWRAIDGHPFLRELAAGTLPLNRFTYFIL
                     QDYVYLLDFAQVLCLGGARAPNIEILELFCRHALGAVEVERSFHASFGKTLGLSRKQL
                     DAVPKGPRTQAYIDHLQSVARGGSLAELVAAVLPCYWIYGETGKRLFKNRPATPKIYR
                     QWIETYASDAFWRPVREQIRLMDFIGAAASPAEKKRMLELFILSSRYEYWFWEQAYLL
                     ESWPV"
     gene            complement(1499..1873)
                     /locus_tag="VE170_06810"
     CDS             complement(1499..1873)
                     /locus_tag="VE170_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15183.1"
                     /translation="MVIRYASAVVTIAGILALASGLVFWSGRATGLLSMHMLLGFLTV
                     IGLWTIGVAQALSAGGSWGLAALAFVVGTLTIYIGMNQAALFPGEHHWLIQLSHLILG
                     ILSIGIGHMAAARQRRQTSRSL"
     gene            2169..2711
                     /locus_tag="VE170_06815"
     CDS             2169..2711
                     /locus_tag="VE170_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15184.1"
                     /translation="MHPIFIVLIPMVLIVIGMVLNSMGLSFDQPPSSAEQDATKRLTI
                     ERDSFRQFFDRQRITVMNRQKRVGQFAWLLLIATAGSFIWLYNYTVDKTVALNRIASL
                     QTLGAQEVKDLILSVTLVDGNNVKYIIKVPAADQKSDPKVKEAISTEKVSSWEMEKLT
                     TALSIGDNVLPMGVALKIAN"
     gene            2835..3125
                     /locus_tag="VE170_06820"
     CDS             2835..3125
                     /locus_tag="VE170_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15185.1"
                     /translation="MADETKPLFVGIKISKALQNDLDSPAPGTKHYFEGNENKDYLQI
                     IDVGEDKFIGRYIKSGYPAAGITDVSRNICSIIKLIARGRRIEENDVHIYSS"
     gene            3184..3621
                     /locus_tag="VE170_06825"
     CDS             3184..3621
                     /locus_tag="VE170_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15186.1"
                     /translation="MEEKHEIGLRDGTVVRHKNKGYEGKIEGTTAIKACFTRAGMLLD
                     TPLSKEAFQYRVVVTGETARCIAPAEDLEILDATSAIICVRCQRNFNTKPGLAGKPGG
                     RCACGGWICPACQGCRVEESEDKKKSCLNQRKRFLRKTAILKK"
     gene            complement(3670..>4128)
                     /locus_tag="VE170_06830"
     CDS             complement(3670..>4128)
                     /locus_tag="VE170_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ15187.1"
                     /translation="VDAAGGDGPDRTYSTAGTNDFVPRHRVEVAEVVVVSQFYARLIV
                     LNGKGRKLTIDEQEQKARQQAVIDNFKPACICNKIRRGTVAKAIQGGAKTFEQVSKRT
                     GVGTGPCGGRRCGSLVRGMLGEPVTTCNDCGWTVLVGAACPRCEYAKQEI"
BASE COUNT          933 a         1079 c         1177 g          939 t
ORIGIN      
        1 cagggttctt atttgaaaat tctttcgtcg atctccagca ccctgaccca taccttaatc
       61 gcccatccga gcattaagcg cgccgaagag ttgcgcggca aacgattcgg tattcaaaat
      121 cccggcggca ccacctggat aaataccatc ctggcgttag agcatgtcgg tttggagcca
      181 aagcgggata atatcaattt gctgccgatc ggcgattcgg ttttagtcgg ccaggctctg
      241 gaggccggca gagtcgatgc ggcggtgctg gacggcgcgc tggtgcgccg gctgcgcagc
      301 aaaggattcg ctatcatcgc ggaattgcag ccggcgaaga ttcctttgct cagccaggcg
      361 atcgttgtcg cgcccgagtt tctgcaaaag cgtggggaca tcaccgagaa actcctcatg
      421 acgctagtcg agagcctggc ctacagcatg gcgccgatga acaaagcggt ggtcatcaaa
      481 actatgatgc gcaagtttca gatcgcggat ccggcggtgg cggaggaggg ctatcatgat
      541 tatttgaacg gtgtcgagcg ccggccgatc cccacccccg atggcatgcg caacattcgc
      601 cggctgatgg ctacgctcaa tccgaagatt gccagtgtca aagtcgaaga tctgatcgat
      661 agccgcttga tccgcaaact cgatgagaac ggcttcatcg ataaaatggc ggtgaactac
      721 ggattaaaat agtcgtgaca cgagttgggc attcgctgct attgatcgac cgcgctattt
      781 ttcacgccag attttcgtta aggctaacct atggcaaaaa gatttagcgc cgaactacgg
      841 caacagagcg agtctatctg gcgggcgatt gacggccatc cgttccttcg tgagttggcg
      901 gccgggactt tgccgctcaa tcggtttacg tacttcatcc tgcaagacta tgtttatctg
      961 ctcgacttcg cgcaagtcct ctgtcttggc ggggccaggg cgccgaacat cgaaatttta
     1021 gaactcttct gccggcatgc cttgggcgcg gtggaggtgg aacgcagctt ccacgcgagt
     1081 ttcggcaaga cgttaggcct gtcgcgcaaa cagctggatg cggtgccaaa agggccgcgc
     1141 acccaggctt acatcgatca cttgcagtcg gtggcgcgcg gcggcagtct ggctgagttg
     1201 gtggccgcgg tgttgccttg ttattggatc tacggcgaaa ccggcaagcg cctattcaaa
     1261 aaccggcccg ccacgccaaa gatctatcgc cagtggatcg agacctacgc gtcggatgct
     1321 ttttggcggc cggtacgcga gcagattcgc ctgatggatt ttattggcgc ggcggccagc
     1381 ccggctgaga aaaagcgcat gctggagttg ttcattctca gcagccgcta cgagtattgg
     1441 ttttgggaac aggcttatct tttggagagc tggccggttt agcattcaaa tctaaaagct
     1501 agagggatcg gcttgtctgc cggcgctgac gcgcggcggc catatgaccg atgccgatgc
     1561 tcaagatgcc caggattaag tggctaagct gaatcagcca gtgatgttct cctgggaaca
     1621 gggctgcttg attcatgccg atgtagatcg ttagcgtgcc aacgacgaat gcaagcgccg
     1681 ccagacccca gcttcctccg gcgcttaggg cttgagcgac accaatcgtc catagtccga
     1741 ttacggttaa aaagcccagc aacatatgca tcgacagtag acctgtcgcc ctgccggacc
     1801 agaaaactaa gccggaggcg agggcgagta ttcccgcaat ggtcacgaca gccgatgcat
     1861 aacgaatcac catggggcac cttgtataga cttgggctgg cggtattgca atcccgttta
     1921 cccgaggact acacggaatg acaaaacaaa gtggttggac ctaagtttgc ttcatgccag
     1981 gtgttcctat agagtactta agatcatgtc tcgatcctat tggcgcgcaa gaggtcgaag
     2041 accggggcta cactttagaa tggcatggtt ggcggcgtcg atgctgtgcc gtaagatttc
     2101 ccggtcttgc accccatccc tcttcctata ttcgcggatt tcaagcatcc gtttgcggag
     2161 ggagtaatat gcatcctatt tttatcgtgc tcatacccat ggttttgatt gttataggta
     2221 tggttttgaa cagcatgggc ttatcgttcg atcaaccgcc gagctccgcc gagcaggacg
     2281 caaccaaacg cttgaccatc gaacgagaca gcttccgaca gtttttcgat cgccagcgta
     2341 taactgtcat gaaccggcag aagcgggtcg gacagttcgc gtggttgctg ctgatcgcga
     2401 ccgcaggctc gtttatctgg ttgtataact atactgtcga caagaccgtg gccttgaatc
     2461 gtattgcgtc attgcagacg ctgggggcgc aagaggtcaa ggatctaatt ctttccgtga
     2521 cactggtcga cggcaataac gtcaagtaca ttatcaaagt tccggcagcc gaccagaagt
     2581 ccgatccgaa ggttaaggaa gcgatttcga cagagaaggt ttcttcttgg gagatggaaa
     2641 agctaaccac cgccttgagc atcggcgata atgttttgcc aatgggcgtt gccttgaaga
     2701 ttgccaactg acggttgacc cagcggcgtt tcatggtggg cgcggtgatc gaggggttgt
     2761 tcgctgccgt tacgggctag tgcaatcccc tggtcgtggt gtaagcgtgc gacgcttgcg
     2821 aagggagctg tgcgatggct gacgagacca agcctttgtt cgttgggatc aagatatcga
     2881 aggcgttgca gaacgacctc gatagccctg cgccgggcac gaaacattat ttcgaaggca
     2941 acgagaataa ggactatctt cagatcatcg acgtcggcga ggacaaattc atcggtcgtt
     3001 atatcaaaag cggttatccc gcggcgggga tcacggacgt gagccgcaac atttgcagta
     3061 tcatcaagct catcgctcgc ggccgccgga tcgaggaaaa tgacgtgcat atttattcct
     3121 cttaagggct gaaagtctta tgacatggca gagaacctga ggctttttac aaagaaggtg
     3181 tctatggaag aaaaacatga gatcggtttg cgcgatggga cagtcgtgcg ccacaagaat
     3241 aagggatacg aaggcaagat cgaaggcacg acggcgatca aggcctgctt caccagggcc
     3301 gggatgctgc tggatacgcc gctctcgaaa gaagcatttc aatatagagt ggtggtcacc
     3361 ggtgaaactg cgcggtgcat cgctcccgct gaagatctcg aaatcctcga tgccaccagc
     3421 gcaattatct gcgtccgttg tcaaaggaac ttcaatacca aacccgggtt agccggcaaa
     3481 ccgggcggac gatgcgcttg cggcggctgg atctgccccg cttgccaggg ttgccgcgtc
     3541 gaagagagcg aagacaaaaa gaagagctgt ctcaatcaac gcaaacgttt cctgcgtaag
     3601 acggccattc tgaagaaata gccaattaac gggacgacag ttgcgctacc ttgtagctaa
     3661 tcagcttgcc tagatttctt gtttagcgta ttcacaccgc gggcaagcgg ctccgaccaa
     3721 caccgtccag ccgcagtcat tgcaagtcgt caccggttcg ccgagcatac cgcggaccag
     3781 agaaccgcag cgccgcccgc cgcaggggcc ggtgccgacg ccggtgcgtt tactgacctg
     3841 ttcgaaggtt ttcgccccgc cttgaatcgc tttggcaact gtgccgcggc ggattttgtt
     3901 gcagatgcag gcgggtttga agttgtcgat gaccgcttgc tgccgggctt tttgttcttg
     3961 ctcgtcgatg gtcaatttcc tgcctttgcc gtttaataca atcagtctag cataaaattg
     4021 gctcacgact actacttctg ctacttccac ccgatgacga gggacgaagt cattggtgcc
     4081 ggcagtgcta tatgttcgat ccggtccatc cccgcctgcc gcatccac
//
LOCUS       DASQKS010000263         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5171731, whole genome shotgun sequence.
ACCESSION   DASQKS010000263 DASQKS010000000
VERSION     DASQKS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5171731"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VE170_06835"
     CDS             complement(<1..339)
                     /locus_tag="VE170_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYJ15188.1"
                     /translation="MADVIWVYAEITDDRITATSLEMLAKAAQLAKAEAVLLGPAPEE
                     AVETLAKYGASKIYRCADPIYGDYLTLPAADTVAELIHKYKPAVMLFASSYSGRDLVG
                     NLSARFDCGAI"
     gene            complement(341..1120)
                     /locus_tag="VE170_06840"
     CDS             complement(341..1120)
                     /locus_tag="VE170_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYJ15189.1"
                     /translation="MNVVVCGKVIPASTVIIELDANTKRMVRKGVPHELDPAAASAVE
                     EGLRLTEKHGGTVVLITMGTSDVTIGIRNALAMGVTSAVHILDDAVAGSDTLATAKLL
                     AAAIKKQQFDIVLCATESGDSYSGIVHGQIAQLLGIPPLTFAKEITVDGDNVTIKRQS
                     EAGYDLVGSTLPALVACSSGINEPRYPQLKGIMAAKKKEIKVYSAADLGLAPDQVGEK
                     GAREKVLTVGRPPKRQAGKAVTDEGEGGKQIADFLAEIKVI"
     gene            complement(1239..1430)
                     /locus_tag="VE170_06845"
     CDS             complement(1239..1430)
                     /locus_tag="VE170_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15190.1"
                     /translation="MEAAANTEALDQQFKEFIQGIKKAVGAGIPHLKDDPKIVDQAIA
                     DCKEILDVVIEGDVKQWLS"
     gene            complement(1493..2215)
                     /locus_tag="VE170_06850"
     CDS             complement(1493..2215)
                     /locus_tag="VE170_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HYJ15191.1"
                     /translation="MFTDKVDLKDFEKQDPEYQDLIRRVLAIQADCEIGGPNLYVKHI
                     LPTAPTKLDQLIVARTAAEEMDHFRKMAKLAGDIGVDVSYTLSRTNQERYVEAFRGEI
                     KTWEDYAVFGFLIDRVGRFQLEEFLDCTYTPLARVVEDPDIIREEEGHLDFGTNATAD
                     FAAKGGEFKDRVQKAVDYWYVKGLDMFGNSKSYRSERYMHWGLKRRSNSVARQQYKDE
                     IDALILKMGLTLPDPNKGRLYT"
     gene            2359..3708
                     /locus_tag="VE170_06855"
     CDS             2359..3708
                     /locus_tag="VE170_06855"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HYJ15192.1"
                     /translation="MASQENLTLLTDLYQLTMAQGYFREQRLGTATFSLFIRSYPPNR
                     GYFVCAGLHDVLDYLQNLSFDNAALDYLAAQRLFTDDFLHYLVDLKFTGEVWAIPEGR
                     IFFTDEPVLEITAPVIQAQIAETFVINQVHLQSLIATKAARCVHAAGGRAVVDFALRR
                     THGTDAGMKVARASYLAGFSGTSNVMAGKDYGVPIVGTMAHSFVMTFEREIDAFRAYA
                     ASFPRATILLIDTYDTLAGARNASTVAGEMASRGEKLIGVRIDSGDLPAQARAVRQIL
                     DEAGLGEVKILGSGGLDEYDLGDFSLAQVPFDSYGVGTKMGTSADAPWADMSYKLVEY
                     AERPVQKLSTGKISWPAKKQVYRRLDAEGHLGSDVIGLRADDIAGAEPLLRKVMDRGK
                     ALRLANLADSRSAFLEEFKRLPEAVKAICSPARYPVEFSPALQKLRAEVESGIANKD"
     gene            complement(3770..>4314)
                     /locus_tag="VE170_06860"
     CDS             complement(3770..>4314)
                     /locus_tag="VE170_06860"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYJ15193.1"
                     /translation="LNWKDRGTCILFGDAAGAVVLGASENGSGVLSTKLRTDGSYVKQ
                     LYVPAGGSLKPPCRETVRNNEHTITMNGKEVFKIAVRSMEEISRQALDEAGVSIDQVA
                     LVIPHQANKRIIVALAERLGVPMDKVVINVDKYGNTSAASIPVALDEARRAGRIKPGD
                     IVLFNAFGAGFAWGAAVVKF"
BASE COUNT          891 a         1274 c         1165 g          984 t
ORIGIN      
        1 gatggcgcca caatcaaatc tcgcgctcag attcccgact aaatcacgtc cgctgtaact
       61 cgacgcaaat aacatcaccg ctggtttata tttgtggatc agttcggcaa cagtatcggc
      121 tgcaggtagc gtgaggtaat caccataaat cggatcagcg cagcgatata ttttgctcgc
      181 tccatatttg gcaagtgtct cgacagcttc ttccggagca gggcccaaga gcacggcttc
      241 cgctttagcc agttgcgccg ctttggcaag catttccaag ctcgtcgccg tgattctatc
      301 gtcggttatt tccgcataca cccagataac atctgccact ttagatcacc ttgatttcag
      361 caagaaagtc cgcgatttgc ttgccgcctt ccccttcgtc ggttaccgct ttaccggctt
      421 gccgcttggg tggccggcct actgttaaaa ccttttcccg cgcgcctttt tcgcccacct
      481 ggtccggcgc tagaccgaga tcggcggcgc tatagacttt gatctctttt ttcttcgccg
      541 ccatgattcc cttcaactgc ggataacgcg gttcgttgat gccgctactg caggccacca
      601 atgccggcaa cgtcgatccg accagatcat agcccgcctc gctctggcgt ttgatcgtga
      661 cgttgtcgcc atcgacggtg atttccttgg caaaggtgag tggtggaatg cccaagagct
      721 gagcgatctg tccgtgaacg ataccggaat aactatcgcc gctttcagtg gcgcacagca
      781 cgatatcgaa ttgctgtttt tttatcgccg cggccaacag ctttgccgtc gccagcgtgt
      841 cggaacccgc taccgcgtcg tcaagtatgt gcaccgccga cgtcacaccc atcgccagcg
      901 cgttgcgtat gccaatggtc acgtcgctcg tgcccatggt gatgagcacg acagtgccgc
      961 catgcttttc cgtgaggcgc aaaccttctt cgacggcgct ggccgctgcc gggtccaact
     1021 cgtggggcac acccttgcgc accatgcgtt tggtattggc gtcgagctcg atgatgaccg
     1081 tgctcgcggg aatgacttta ccgcagacga cgacgttcaa tgttttgctc tcccgtgcat
     1141 gaattcgcct gttacagata cgacaaaggc gcctttggga caaggcgcct ctggtcgcga
     1201 gactgatttg atgcgcttaa gttttgccga tcagcggctc atgaaagcca ctgcttgaca
     1261 tcgccttcta tgacgacgtc aagaatttcc ttgcagtcgg cgatcgcctg gtcgacgatt
     1321 tttggatcgt ctttcaggtg cggtatgccg gcgcccaccg ctttctttat gccttgaatg
     1381 aattctttga actgctggtc caacgcttcg gtatttgctg ctgcttccat gacactcctt
     1441 gcgggtcgcg gtatgcgtct cgagtgaaac gcgaccgcga cgctcgtatt aattatgtat
     1501 aaagccggcc cttgttagga tcgggaagcg tgagccccat ctttaggatc agtgcgtcga
     1561 tttcgtcctt gtactgctgg cgcgctactg agttggaccg ccgtttaagc ccccagtgca
     1621 tatagcgctc ggaacggtac gacttgctgt tgccgaacat gtcgagccct ttgacgtacc
     1681 agtagtcgac cgccttttgc actctatcct taaactcgcc gcctttggcg gcgaaatcgg
     1741 cggtggcgtt ggtgccgaag tcgagatgac cttcctcttc gcggatgata tccggatcct
     1801 cgaccacgcg ggcgagcggc gtataggtgc agtcaagaaa ttcttcaagc tggaagcggc
     1861 cgacgcgatc gatcaagaaa ccgaagacag cataatcttc ccaggttttg atctcgccgc
     1921 gaaacgcttc gacgtaccgt tcctgattgg tccggctgag cgtatacgaa acatcgacgc
     1981 cgatatcgcc ggccaacttt gccattttac ggaaatggtc catctcttcg gcggccgtgc
     2041 gcgccacgat caactggtcc aacttggtcg gcgccgtcgg caaaatatgc ttgacataaa
     2101 gattcgggcc accgatctcg caatcggctt gaatcgcaag cacgcggcga atcagatcct
     2161 ggtattcagg atcctgtttt tcgaaatcct tgagatcgac cttgtcggta aacatcgatg
     2221 cctcctatgg gatgtaatta tgcgtaagct atacaaaaat tcttaacaaa acggtttcga
     2281 caaatcaacc ggagcggctt gccaggatta cccgaaccgg ctatggactg actgagcaag
     2341 ggtgctcgaa agcaatccat ggcaagccag gaaaatctca cactgctcac cgacctctac
     2401 cagctaacca tggcccaagg ctactttcgc gagcaacggc tcggcacagc gacgtttagt
     2461 ctatttatcc gcagttatcc gcccaaccgc ggctatttcg tctgtgccgg gctgcacgac
     2521 gtcctcgatt atctacaaaa cctatccttt gataacgccg ctctcgatta tctcgcggcg
     2581 caacgtcttt tcaccgatga ctttctccat tacttggtcg atctcaagtt caccggggag
     2641 gtgtgggcga ttcccgaagg acggatattt ttcacagacg aaccggtcct cgaaatcacc
     2701 gcgccggtga tccaggcaca gatcgccgag accttcgtca tcaatcaagt tcatctgcaa
     2761 agcctgatcg ccaccaaagc cgcccgatgc gtgcatgccg cgggcgggcg ggcggtggtc
     2821 gacttcgcgc tgcgccgcac ccatggcacc gacgccggca tgaaggtggc gcgggccagc
     2881 tatctcgccg gtttttccgg caccagcaac gtcatggccg gcaaagacta cggtgtgccg
     2941 atcgtcggca ccatggcgca ctcgtttgtc atgaccttcg agcgcgagat cgacgccttc
     3001 cgcgcctacg ccgcaagttt tccgcgcgcg acgatcctgt taatcgacac ctacgatact
     3061 ctcgccggcg cgcgcaacgc ttccaccgtc gccggagaaa tggccagtcg tggcgaaaag
     3121 ctcatcggcg tgcgcatcga cagcggtgat ctgccggcac aggctcgcgc cgtgcggcag
     3181 attctcgatg aagccgggct tggcgaggtc aaaatactcg gcagcggcgg cttggacgaa
     3241 tacgatcttg gcgatttttc ccttgcacaa gttcccttcg acagctacgg cgtcggcacc
     3301 aagatgggca cttcggccga tgcaccgtgg gccgacatgt cttataaact ggtcgaatac
     3361 gccgaacggc ccgtgcaaaa acttagcacc ggaaaaattt cctggccggc aaagaaacag
     3421 gtatatcgcc gcctcgacgc cgagggtcat ctaggcagcg atgtcatcgg cctgcgcgcc
     3481 gacgatattg ccggtgccga accgctgctg agaaaagtaa tggatcgagg aaaagcgctg
     3541 cgactggcta atttggcaga cagccgctcg gcattcttgg aagaatttaa gcgcctgccg
     3601 gaagccgtca aagcaatttg cagtcccgcg cgctatccgg tggagttttc ccccgccctg
     3661 caaaagctgc gcgctgaagt agaaagcggt atcgcaaaca aggattgaat gtgaccgaga
     3721 aacctagaca gcttgaacca cttgaacgtt ttgaacggct tgaacaagct cagaatttga
     3781 ccaccgccgc gccccaagcg aagccggcac cgaaagcgtt gaacaggaca atgtcgccgg
     3841 gcttgatgcg gccggcgcgt ctggcttcat cgagggcgac cggtatcgat gccgccgacg
     3901 tattgccgta tttgtcgaca ttgatcacga ccttatccat cggcacgccg agccgttcgg
     3961 ccagcgcaac gatgatgcgt ttgttggcct ggtgggggat gactagagcc acctgatcga
     4021 tgctgacacc ggcttcgtcg agcgcttgcc ggctgatctc ttccatggaa cgcacggcga
     4081 tcttgaatac ctctttcccg ttcatggtga tcgtgtgctc gttgttgcgc accgtctccc
     4141 gacagggagg cttgagcgaa ccgccggcgg gaacgtagag ctgtttaacg taggaaccgt
     4201 cggtgcgcag tttggtgctc aagacaccgg agccgttttc ggacgcgccc aacaccaccg
     4261 cgccagcggc gtcgccaaaa agtatgcagg tgccgcgatc cttccagtta agca
//
LOCUS       DASQKS010000264         6196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5171764, whole genome shotgun sequence.
ACCESSION   DASQKS010000264 DASQKS010000000
VERSION     DASQKS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5171764"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VE170_06865"
     CDS             <1..749
                     /locus_tag="VE170_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ15194.1"
                     /translation="IGDSGTGIHLVAGILAALFQREKSGQGQRVTCAMQDTVLNLCRV
                     KLRDQQRLAAGPLTEYPQYPNGVFGDAVPRAGNASGGGQPGWAVKTKGGGPNDYIYVI
                     IQPPGWEPLMKLCGRPELIADPEWATPEKRLPKLDACFAIVEQWAMTLDKLAVMRELN
                     KFDIPCGPILDMREIAEEQSLRDTGTVVEVDHPERGKFLTVGNPIKLSDSPTEIIRSP
                     LLGEHTDEILKSVVGWSDAEIAAARKQGAV"
     gene            954..3074
                     /locus_tag="VE170_06870"
     CDS             954..3074
                     /locus_tag="VE170_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYJ15195.1"
                     /translation="MDRKGLSILIDSVRKSGRRALSALEAQQLCDSYGIPTPKQELAK
                     SAAEAAKIAARLRFPVVLKIVSDDILHKTETGGVIVGVKSASEARRAFDRLIKGAKAY
                     NKHAAIQGVLVQKMVGAGREVMVGAVTDTAFGKLVAFGLGGVLVEVMKDITFRLAPVS
                     KQDALAMLDGISGAKILRGVRGAKGVDRAALADIIQKVSKLVNDFPEIVEVDLNPIFA
                     TDKGAHAVDVRIVVGAPLPQRQRFSRSEILSAMQRIMNPRAVAVIGASAEDGKIGNSV
                     MKNLINGGYAGRIYPIHPKADEILGRKAYASVLNVPEEIDVAVFAIPAKFCAGAMEEV
                     GRKQIPGAVMIPSGFAEVGEQGLQDELLGVAQRHKVRIMGPNIYGFYYTPQNLCATFC
                     TPYDVKGKVALSSQSGGVGMSIVGFSRTTRMGVSAIVGLGNKSDLDEDDLLTYFEQDD
                     NTQVIAMHCEDLKDGRSFAQVASRVSKKKPVIMLKAGRTALGARAAASHTGALAGNDK
                     IYDDVLRQCGVIRAKSLNDLLQFARGLQVLPTPKGGNIIIITGAGGSGVLLSDACVDN
                     DLSLMKMPPDLDAGFRKFIPPFGAAGNPVDITGGEPPTTYANTIRYALAEPRIHALIL
                     GYWHTIITPPMVFAKLCAEVVEEARIKGIDKPVVASLVGDVEVEQACEYLYERGIVAY
                     PYTTELPVAVLGAKYKWARGAGVL"
     gene            3268..4368
                     /locus_tag="VE170_06875"
     CDS             3268..4368
                     /locus_tag="VE170_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ15196.1"
                     /translation="MAQQTLTAVVTGKRQIEMRYFPVPKPSVADAILKVELCGICGSD
                     YHYYIEGHHWPYQPPPQILGHEVVGRINDISSAAAKHWKVKEGDLVIVESAVTCHRCR
                     YCTTGNSSLCSTKRSYGMSASMNEPPYLWGGYSQYMYLHPDAILHVVPPGVSEHEAVL
                     FSGLANGVKWAQRIPALSLGDAIVILGPGQQGLGCVLASSMTGANPIIVAGLERDAKR
                     LEIAKTLGATHTIFSDKAPLLEQVRNITGPELADVVVDVTGSTAAQEVAVDLVRRGGT
                     VVLGGRTPKKTISFVMDKLTSRGIKMVGVRAHDSDDVRKAVAVISARRHQLANLLTHE
                     VSLREADLALRLIGQEVPGEEAIHVAINPWRE"
     gene            4413..5513
                     /locus_tag="VE170_06880"
     CDS             4413..5513
                     /locus_tag="VE170_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ15197.1"
                     /translation="MDKKSLAAVIVDKRRMEMREFAVPDPGADDAVLKVEACGICGSD
                     YHYYKQLDHWPYLQPPHIMGHEVVGRIGAIGRDASRQWNLKEGDLVAVEAPVTCQRCR
                     YCLTGNATLCADKRSYGISASMADPPHLSGGYAQYMYLRPNSILHPVPAGVTPQAAVL
                     YTCVSNGLKWAQRVPALSPGDSIVILGPGQQGLGCVLAAAMAGAAPIVVAGLEADAAR
                     LAVAKELGASHLIYSERGPLAEQVKNILGPEMADVVVDVTGSVSAQLATVDLVRRGGT
                     IVLAGRTPNQTVQFEIDKVASRGIVMIGVRGHESDDIRRALSVIAARPEAAARLTTHE
                     VPLHDADYALRLIGQEIPGENAIHVSLNPWLS"
     gene            5527..>6196
                     /locus_tag="VE170_06885"
     CDS             5527..>6196
                     /locus_tag="VE170_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15198.1"
                     /translation="MRWVNLFGHERSPINKTIAAFGGLLLFILVSSTANAGRLRAGFA
                     SPSLNVAMLWITQEGRLFEKNGVDVEALYLESTLAQKALIVGNIDFSMMTAINMAAPK
                     LAGADLIMLAGFVNRFTYRLVVRPEINATSDLKGKRIGIFRFGSAADRASRLVLAKFG
                     LDPEKDVTYVQTPGADPARIAAMMSKIIDGALLNPPYYKHAVAGGMKILANMAEMDIP
                     IQHIG"
BASE COUNT         1279 a         1828 c         1872 g         1217 t
ORIGIN      
        1 agatcggcga ttccggcaca ggtattcacc tcgtcgcagg catactcgcg gcgctttttc
       61 agcgcgagaa gagcggccag ggccagcgcg tcacctgcgc catgcaggac acggtgctca
      121 atctctgccg ggtgaaattg cgcgatcagc agcgcttggc ggctggaccg ctgacggaat
      181 atccgcagta tcccaacggc gtttttggcg atgccgtgcc gcgcgcgggc aacgcttccg
      241 gcggcggcca acccggttgg gccgtgaaga ccaaaggcgg cggtcccaac gattatattt
      301 acgtcatcat tcagccgccc ggctgggagc ccttaatgaa gctgtgcggc cgccccgaat
      361 tgatcgccga tcccgagtgg gcgacgccgg agaaacgatt gcccaagctc gacgcgtgct
      421 tcgccatcgt cgaacaatgg gcgatgacgc tcgacaagct ggcagtcatg cgcgagctca
      481 ataagttcga cattccctgc ggaccgatcc tggacatgcg cgagatcgcc gaagaacaat
      541 ccctgcgcga caccggtacc gtggtcgaag tcgatcatcc cgagcgcggc aagtttttga
      601 ccgtcggcaa tcccatcaaa ctttccgatt caccgacaga aataattcgt tcaccgctgt
      661 tgggtgagca taccgatgaa attttaaaat ccgttgtcgg ctggagcgac gcggagatcg
      721 ccgcagcgcg caaacagggc gcggtttaga gcacaggact caccacaggg tgcccagcga
      781 tcataggcta ggggaatcgg agtcggaatt tagccgcaga gaacgcaaag ggcgcaataa
      841 gaattgatca tcgtcggggc gggttttaaa cccgccctct tggttttaac tcggtgaaaa
      901 atttacccga gccgattaac tactatcaat atagcaaagc atcggaggca ttgatggaca
      961 ggaaaggctt gagtatcctc atcgactcgg ttcggaagtc cggccggcgc gctttgagcg
     1021 cgctcgaagc ccagcaactg tgcgatagct acggcattcc cacgcccaag caggagctgg
     1081 ccaaaagcgc ggcagaggcc gcgaaaatag ccgcgcgttt gcgcttcccc gttgtcctca
     1141 aaattgtttc cgacgatatc ctccataaga ccgaaaccgg cggcgtgatc gtcggtgtca
     1201 aaagcgccag cgaagcgcgc cgcgctttcg atcgcctgat caaaggcgcc aaggcgtata
     1261 acaagcacgc cgcgatccaa ggggttctgg tacaaaagat ggttggcgcc gggcgcgagg
     1321 tgatggtcgg tgcggtgacc gatacagcat tcggcaagct agtcgccttc ggcttgggcg
     1381 gtgtcctcgt cgaagtcatg aaggacatta cctttcgcct ggcgccggtg agcaagcaag
     1441 acgcgctggc catgctcgac gggatcagcg gcgcgaaaat tctccgcggc gtgcgcggcg
     1501 ctaaaggagt ggatcgcgcg gcgctcgccg acatcatcca aaaagtatcg aagctggtca
     1561 acgatttccc cgagatcgtg gaagtcgatt tgaatccgat ttttgccacc gacaaaggcg
     1621 ctcacgcggt ggacgtgcgc atcgtcgtcg gtgcgccgtt gccgcaacgc cagcgtttca
     1681 gccggagcga aatattgagc gccatgcagc ggatcatgaa tccgcgcgcc gtcgccgtca
     1741 ttggtgcgtc ggcggaagac ggtaaaatcg gcaattcagt catgaagaac ttaatcaacg
     1801 gcggctatgc cggcaggatc tatccgattc atccaaaggc cgatgaaatc ctcgggcgca
     1861 aggcttatgc gagcgtgctc aacgtgccgg aagaaattga cgtcgcggtg ttcgcgatcc
     1921 cggcaaagtt ttgtgccgga gcgatggaag aggtggggcg caagcaaatt cccggggcgg
     1981 tgatgattcc gtccggcttc gccgaggtcg gcgaacaggg tttgcaggat gagttgctcg
     2041 gagtggccca gcgccacaag gttcggatca tgggaccgaa catctacggc ttttactata
     2101 cgccgcagaa cctttgcgca actttttgca ctccctacga tgtcaaaggc aaagtcgcgc
     2161 tcagttcgca atccggcggc gtcggcatgt cgatcgtcgg tttctcgcgc accacccgca
     2221 tgggcgtgtc ggcgattgtt ggcctcggca acaaatcgga tctcgacgaa gacgatctgt
     2281 tgacctattt cgagcaggac gacaacaccc aggtgatcgc catgcactgc gaggatttga
     2341 aagacggccg atcgttcgcc caagtggcga gccgggtgtc gaagaagaag ccggtgatca
     2401 tgctcaaggc cggccgcacg gcgctggggg cgcgggccgc ggcgtcccac accggcgcgc
     2461 tagcgggcaa tgacaagatc tacgacgacg tcctgcgcca atgcggtgtg attcgcgcca
     2521 agagtttaaa cgacttgctg caattcgccc gcggcttgca ggtgctgcca acgcccaagg
     2581 ggggaaatat tattatcatt accggtgccg gcggttccgg tgtgctattg tcggatgctt
     2641 gtgtcgataa tgatctgtca ttgatgaaaa tgccgccgga tctcgatgcc ggattccgca
     2701 agttcattcc gcccttcggc gccgccggca atccggtcga catcaccggt ggcgagccgc
     2761 cgactaccta cgccaatacc attcgctacg ccctcgccga gccgcgaatt catgcgttga
     2821 tcctgggcta ctggcacacg attatcacgc cgccgatggt attcgccaaa ctttgcgccg
     2881 aagtagtcga ggaagcgcgg atcaaaggca tcgacaaacc ggtggtcgct tcgctggtcg
     2941 gcgatgtcga agtcgaacaa gcctgcgagt atctgtacga gcgcggcatc gtcgcttatc
     3001 cgtacacgac ggaactaccg gtcgccgtac tcggcgccaa gtataaatgg gcgcgcggcg
     3061 ccggcgtgct ttgatcgcag agctggctat gccaacggct aattttaatt taattaagcc
     3121 cactttttac cgtcccgcga taattgtctt gcggtcgttt ctaaccagac cgtaggcttt
     3181 caatagccct ggtgtttggc gtaatactca ctgtaaccgt gaagtccctg acctttttgc
     3241 ttgcctagct aacgaacgga gaccccgatg gcacaacaaa ccctcaccgc agtcgttacc
     3301 ggcaaacgcc aaatcgagat gcgctatttt ccggtgccca agccctctgt ggcggacgcg
     3361 atcttgaagg tcgagttgtg cggtatttgc ggcagtgatt accactacta catcgaaggc
     3421 caccattggc cgtaccagcc gccgccgcaa attctcggtc atgaggtggt cgggcgcatc
     3481 aacgatattt ccagcgcggc cgccaaacac tggaaagtta aggaaggcga cttggtcatc
     3541 gtcgagtccg cggtcacttg ccatcgctgc cgttactgca ccaccggcaa ttcttcgcta
     3601 tgctcgacca agcgcagcta cggcatgagc gcgagcatga atgagccgcc gtatttatgg
     3661 ggagggtatt cccagtacat gtatctgcat cccgatgcga ttcttcacgt cgtgccaccc
     3721 ggagtctccg aacatgaggc cgtgctattc agtgggctgg ccaacggggt caaatgggcg
     3781 caaaggattc cggcgttgag cctcggcgac gccatcgtca ttctcggtcc gggccagcag
     3841 ggtttaggct gcgtcctggc gagttcgatg acgggcgcca atccgatcat cgttgccggt
     3901 cttgagcgcg atgccaagcg cttggaaatt gccaaaaccc tcggcgcgac gcatacgatc
     3961 ttttccgaca aagcgccgct gcttgaacag gttcgcaaca ttaccggccc agagctggcc
     4021 gatgtcgtgg tcgacgtcac cggcagcacc gccgcgcagg aagtcgccgt cgatctggtc
     4081 cggcgcggcg gcacggtggt gctcggcgga cgaacgccga aaaaaaccat ttccttcgtc
     4141 atggacaaac tcaccagccg cggcatcaaa atggtcggcg tgcgcgccca tgattcggat
     4201 gacgtgcgca aggcggtggc ggtcatcagc gcgcggcggc atcagctggc caatctactc
     4261 acccatgaag tgtcgcttag agaagctgat ttagccttgc ggctgatcgg ccaggaagtt
     4321 ccgggcgagg aagcgattca cgtcgcgatc aatccatggc gtgagtgagt ccgggatcgc
     4381 catggctgca caacccgaac aaggagttaa cgatggataa gaaatccctc gctgccgtca
     4441 tcgtcgacaa acggcgcatg gagatgcgcg agttcgccgt gcccgaccct ggcgccgacg
     4501 acgcggtgct gaaagtggag gcctgcggca tctgcggcag cgactatcat tattacaaac
     4561 aactcgatca ctggccttat ctgcagccgc cgcacatcat gggccatgag gtggtcggcc
     4621 gcatcggcgc catcggccgc gatgccagcc ggcagtggaa cctcaaagaa ggcgatctag
     4681 tcgccgttga agcgccagtg acttgccaac gctgccgcta ctgtctcacc ggtaacgcga
     4741 cgctgtgcgc cgacaagcgc agttacggca tcagcgccag catggccgat ccgccgcacc
     4801 tatcgggcgg ctacgcgcag tacatgtatc ttcgcccaaa ttcgatcttg catccggtgc
     4861 cggccggcgt gacgccccag gcggcggtgc tctacacctg cgtgtcgaac ggtttgaaat
     4921 gggcgcagcg ggtgccggcg ctaagccccg gcgactccat cgtcattctc gggcccggcc
     4981 agcagggcct gggctgtgtc ttggccgcgg cgatggcggg cgcggcaccg attgttgtcg
     5041 ccggtctgga agcggatgcg gcgcgccttg ccgtggccaa agagttgggc gcgtcacacc
     5101 taatttattc cgagcggggg ccgctggcgg agcaggtaaa aaatatactt gggccagaga
     5161 tggcggatgt cgtcgtcgac gtcaccggca gtgtgtcggc ccagctagcg accgtcgatc
     5221 tggttcgtcg cggcggcacg atcgtattgg ccggccgcac gcccaatcag accgtgcagt
     5281 tcgaaatcga taaagtcgcc agccgtggca tcgtgatgat cggcgtgcgc ggccacgagt
     5341 ccgacgacat ccggcgcgcc ctgagcgtca tcgccgcccg gcctgaggcg gcggcgcgac
     5401 tgacgactca tgaagtgccg ttgcatgatg ccgattacgc gctccggctg atcggccagg
     5461 agattcccgg cgagaacgcg attcatgttt cgctcaatcc ctggttgtcc taggcgggag
     5521 gaacctatgc ggtgggttaa cttatttggc catgagcgca gtccaatcaa taaaacgatt
     5581 gccgcattcg gcggcttgtt acttttcatt ctggtttcgt ccacggccaa cgccggccgc
     5641 cttcgcgccg gtttcgcctc gccgtcgctc aatgtcgcga tgctgtggat cactcaggag
     5701 ggcaggctgt ttgagaaaaa cggcgtcgac gtcgaagcgc tttatctgga aagcacgcta
     5761 gcgcagaagg cgctgatcgt cggcaatatt gatttctcca tgatgaccgc gatcaacatg
     5821 gccgcgccca aactcgccgg agccgacctc atcatgctcg ctggtttcgt caaccgcttc
     5881 acctaccgtc tggtggtccg tcccgaaatc aatgctacca gcgacctcaa aggtaaacgc
     5941 atcggcatct ttcgtttcgg ctcggccgcc gaccgcgcca gccgcttggt gctggcgaag
     6001 ttcggtttgg acccggagaa ggatgtcact tacgtgcaaa ctccgggagc cgacccggcg
     6061 cgaatcgccg cgatgatgag caagattatc gacggggcgc tgctaaatcc gccctattac
     6121 aaacatgccg tcgccggcgg catgaaaatc ctcgccaaca tggcggagat ggatataccg
     6181 atccaacata tcggcc
//
LOCUS       DASQKS010000265         9567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1812934, whole genome shotgun sequence.
ACCESSION   DASQKS010000265 DASQKS010000000
VERSION     DASQKS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812934"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VE170_06890"
     CDS             <1..523
                     /locus_tag="VE170_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15199.1"
                     /translation="PHPRFWVGCARTEDSFRWAGEKGFHLMTLPYLYREPGALPAFIK
                     IYRDNLAKAGHDAGNMDILGKFHIYVSTSLEKAIEEAAPYLDNYLVVHQAADTDRKEV
                     GLLIQRDAKTQIAEGFVLAGDPQRVADTIRKWAEPNGLTTISGTFHFGGMPQEMALKS
                     IRLFADKVMPQFK"
     gene            747..1688
                     /locus_tag="VE170_06895"
     CDS             747..1688
                     /locus_tag="VE170_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ15200.1"
                     /translation="MKVKPKRKPIKWPNGARIAITPCVAFETWPEDLGGPDTLQQENR
                     RSFPRNAVTKRNLAIITDREFGERVGIYRMLNVFEQEKIRTTFFPTGITVQNYPEIFK
                     EAAQYGHEIGTETWTHDYSYMKQRAKEKKDLQNTVAAVTKVVGKAPVGYLSTGVAPSA
                     STPEVILECGYNYWMDPQHEETPYTLKVKNKELTVLAYFQDLNDYSSYQRAGRTPRDL
                     LQMWKDCFDCLYEEGATDPKFMMWGNHPFVGGRPFRALLLREFIRYAKSHKKVWFATA
                     GEIVKWYRENYHDAQVEEWPNFHHAGRRGKISDLAKL"
     gene            complement(1788..2711)
                     /locus_tag="VE170_06900"
     CDS             complement(1788..2711)
                     /locus_tag="VE170_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15201.1"
                     /translation="MLLRLLLSSVLWAGTLAHAENIKIGLPSVTITAMPFFVAKEHGF
                     FQQEGLNAEMVVMPASLNIKVLLAGDIQYAATMGSAVAAAIRGINVRIVMLFVDRPLQ
                     DLVGAANIGSISELKGKIVAISSRGGLQDIIMRRILSQSKIDPSQVNVITIPGQTAMI
                     SALKTGRIGAAMLNPPFNFLAYREGLTNLGFSGNFVRLPSTGVATLGETLERSPDQVR
                     RLTRALARARAFAKDNKAKIFPTLKRAMRIEDEDLLSKIYEQHKQVETADGRVDAQLI
                     ADTIRDARQTEGITKEIPANQVFDFSYLPAR"
     gene            complement(2724..3821)
                     /locus_tag="VE170_06905"
     CDS             complement(2724..3821)
                     /locus_tag="VE170_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15202.1"
                     /translation="MAESFATVDADGHLEENHIDWKARLPEKYRSQAPERRDSGNGQL
                     RTIIEDKAWPRPSGLGIGVGGPYSRPHPRRSGMTDPHARLIDMDSEKIDVAVLFGAGL
                     SGTVPALDDAGLAVALATARNTWLAEYCAADPKRIKGTAALAQQDIAASVSELRRCVT
                     QLGMVGASIFPNVKGRHLGDPYYFPLYAEAEKLNVPICVHMFLGRYGSDATGTERVDK
                     FFYSHLFGHPFEQMIALSVILGEGVLDRFPKLRFVFLESGCGWLPYWFHRMDEHYEVL
                     GVQTPELKSPPSKLLERGQLYFSFEADENELAHVIDAFGDEWLVFASDYSHFDSRWPG
                     ASEPIVNHPTLSAATKRKILNDNARMLYPLD"
     gene            complement(3875..4906)
                     /locus_tag="VE170_06910"
     CDS             complement(3875..4906)
                     /locus_tag="VE170_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15203.1"
                     /translation="MLNLKVGVTKNPRFEPLIDGSVKSDKANLNIHVTTPPELFFKNL
                     KNDEFDIFEMSLSEFLITREQAKGDRWQWAALPIFPAKAFVWMTLFVNTAAGIQELCD
                     LKGKRIGIPDYVMTAGLWFRVILRELCGIQPKDISWYIGRVQDLSHGGLLGMDSRPPT
                     DVSTTWLTKEQTFDVMLDKGEIDAAYGFAPRHDTKLMTLNIDRYGGTPLEGNPRLRKM
                     FADGGLSIVDQFYKKTGIVPANHVIVAQRRLLEENSWLAQEIFRLFSESKSLAYQRGN
                     FGNPAYLYFEHSDRASQEKMVGADPFPFGIANNRKMLETLFRNSHEEGLTRRLAKIEE
                     VFFPGLLDT"
     gene            complement(5138..5947)
                     /locus_tag="VE170_06915"
     CDS             complement(5138..5947)
                     /locus_tag="VE170_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYJ15204.1"
                     /translation="MDIGKTMQTIVDVGILIGFKIIGAIIFYLIGRWLISLAIRLVTS
                     ALEKQRVDPTLLRYIGTVISVTLNVILVVAILGYFGVETTSFAALFAAAGVAIGLAWS
                     GLLANFAAGAFMIVLRPFKVGDFVTAASVTGTVKEIGLFVTALVTPDNVLTMLGNSKI
                     FSDTIQNFSANPFRRVDLKAQLAHSVDHNEAIRLLKERVGKVANIKTDPAPDVELLEF
                     NFNGPVLAVRPYCHTDHYWQVYFDTNKVIRETCGAAGYPVPEQHFVVRNPT"
     gene            6173..7060
                     /locus_tag="VE170_06920"
     CDS             6173..7060
                     /locus_tag="VE170_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ15205.1"
                     /translation="MKICHYDNKQAGVVVGEKVINFGDAMIKAGLARQGYTMLEIIDA
                     LANNPAAMQIARDAANGSGGVDLKSVKLLAPIQNPGTLWAAAANYGAHRAEMVDRMGS
                     ANKQILNKDDLMAEFFIKTSSSIIGPGDTVILPKMSKLVDFECELCAVIGKKARKVSE
                     ADSLDHVFGYMMCWDISQRDPWGKGMHNTRNMRKGFDTFTGLGPWIVTRDEIAEPQNL
                     SITVLQNGKEAMIAHTSDMICGLREHIRFLSNCVTLRTGDCITTGTPAGVSKLHDGDK
                     LYGTIEKIGSMELNVKDEA"
     gene            7135..8244
                     /locus_tag="VE170_06925"
     CDS             7135..8244
                     /locus_tag="VE170_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYJ15206.1"
                     /translation="MPAAPKANPPIRTLLGPGPSPVSPRVLAALALPVVGHLDPKFLE
                     IMDQSMAMLRELFQTNNRLALPMSGTGSAGMETCFVNLIEPQDRVLIGVNGVFGTRMV
                     DVAQRCGAQVDTVEAEWGSALDAQKVKEALAKNKYKLVAVVHAETSTGVLQPMDDIAK
                     LVHDSGALLLVDAVTSLGGAPVRVDELGIDACYSGTQKCLGCPPGLSPVTFNDRAVEV
                     IRQRKTKVQSWYLDLSMIEKYWGSERVYHHTAPISMNYALHEALRAVLEEGLENSWQR
                     HKQTHEVFIREMRKLEIEPAVAASIRAPMINAITIPAGADDAKVRTRLYDEYNIEIGA
                     GLGPLKGKIWRVGLMGHGSRVENVETLAKALKKLI"
     gene            complement(8342..9307)
                     /gene="msrP"
                     /locus_tag="VE170_06930"
     CDS             complement(8342..9307)
                     /gene="msrP"
                     /locus_tag="VE170_06930"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HYJ15207.1"
                     /translation="MAVDKRKSWQLSERELTPETFFRQRRQLVKSAGLIGLGVASGLI
                     SACGPAGDAIRVGAQERPAGADLYPARHNPKFTLDRPVTGEAYAASYNNFYEFSVFKS
                     RVYKNAARLTTHPWQVEIAGMVAKPRLYDIDELVRAMGLEERLYRFRCVEAWAMAVPW
                     SGFPLAALIKKAEPLSAAKFVRFVTFLKPDQATNQTLSYGPWPYTEGLTMAEAMNELT
                     LMATGIYGHPLPKQQGAPLRLVVPWKYGFKSIKSIVKIEFTAEQPATFWHSNRPREYT
                     FTANVDPNTPHPRWSQATEKLIDSGERRPTLPYNGYGQWVAKLYG"
     gene            complement(9376..>9567)
                     /locus_tag="VE170_06935"
     CDS             complement(9376..>9567)
                     /locus_tag="VE170_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYJ15208.1"
                     /translation="KYATLLRKEPNFSAPVLATFTIGTKVMVINQSRDWFEVKSHHNG
                     PSGYIRKEFTIPVGVAVYR"
BASE COUNT         2072 a         2565 c         2928 g         2002 t
ORIGIN      
        1 gccgcatccg cgtttttggg tcggatgcgc gcgcacggag gattcatttc gctgggccgg
       61 agaaaagggc tttcatttaa tgacgctgcc gtatctttat cgcgaaccgg gagcgttgcc
      121 ggcgtttatt aaaatatacc gcgacaattt ggccaaggcc gggcacgatg ccggcaacat
      181 ggacatcctc gggaagtttc acatttatgt atctaccagc ctggaaaaag ccatcgaaga
      241 agccgcgcct tatctcgaca attatctcgt ggtgcatcag gccgcggata ccgaccgcaa
      301 agaggtcggc ctattgatcc agcgcgacgc caagacgcag atcgccgagg gttttgttct
      361 ggccggcgat ccgcaacggg tcgcggatac gatacgcaaa tgggctgagc ccaacggact
      421 gacgactatc tccggcacct ttcactttgg cggcatgccc caggagatgg cgctcaagag
      481 cattcgcttg ttcgccgata aggtcatgcc gcagttcaag taggagaaga gtcggattta
      541 ggaattcacc acaaagagcg catagatcac aaagtaaaaa gactcggagt cggaagattg
      601 gccgcagaga acgcaaaagt cgcaaaattg atttccgaac cgtagggcgg gtttaaaacc
      661 cgcccttgct ttatccgccc tcggttggca accggtggga tctatggcat gagtagaaaa
      721 attttttcaa cttcgaggga ggcagtatga aagttaaacc caagcgcaag ccgatcaaat
      781 ggcccaacgg cgcacgcatc gcgatcacgc cctgtgtggc ctttgaaacc tggccggaag
      841 atttgggtgg gccggacacg ttacagcagg agaatcggcg cagtttcccg cgcaacgcgg
      901 tcacaaaaag aaatctggcg atcatcaccg atcgtgaatt cggcgaacgg gtcggcattt
      961 accggatgct gaacgttttc gagcaggaaa agattcgcac gactttcttc cccaccggca
     1021 tcacggtgca gaattatccg gagatcttca aggaagccgc ccagtatggc catgagatcg
     1081 gcaccgagac ctggacccat gattacagct atatgaagca gcgggccaag gaaaagaagg
     1141 acttgcaaaa caccgtcgcc gccgtcacca aggtagtggg caaggcgccg gtgggatatt
     1201 tgtcaaccgg cgtggcgccg agcgcttcga cgccggaagt gatcctggaa tgcggctaca
     1261 attattggat ggacccgcag catgaggaaa cgccgtacac gctcaaggtc aagaataagg
     1321 agctgacggt gctggcctac ttccaggatc taaatgatta ttcgagctac cagcgcgccg
     1381 gccgcacgcc gcgcgattta ttgcagatgt ggaaggattg cttcgattgc ctctacgagg
     1441 agggcgccac cgatccgaag ttcatgatgt ggggcaacca tccgtttgtc ggcggccggc
     1501 cgttccgtgc cttgttgttg cgtgagttca tccgctacgc caaaagccac aaaaaagtct
     1561 ggttcgccac cgccggtgaa atcgtcaaat ggtaccgtga gaattaccac gacgcccagg
     1621 tcgaagaatg gcccaatttc catcacgcgg ggcggcgcgg caagatcagc gatctagcga
     1681 aactctaaag aagagggact ggccgcaaag aacgcaaagt tcgcaaaata ggaagcggat
     1741 ttgttggggc gcagcatgct gcgccccttt gcgttttggg cggttcgcta gcgcgccggc
     1801 aaataagaaa aatcaaaaac ctgattggcc gggatctctt tcgttattcc ttccgtctga
     1861 cgggcgtcgc ggatggtgtc ggcgatgagc tgggcgtcga cgcggccgtc agcagtttcg
     1921 acttgtttat gttgctcgta gatcttcgac agcaggtctt cgtcttcaat gcgcattgcg
     1981 cgtttgagcg tcgggaagat tttcgctttg ttgtctttgg caaacgctct ggcgcgtgcc
     2041 agcgcgcgag tcaggcggcg gacttggtcc ggcgaacgtt ccagtgtctc gcccaaagtc
     2101 gccacccccg tgctcggcag gcgcacgaaa ttgccggaga agcccaagtt agtcagtcct
     2161 tcgcgatagg cgagaaaatt aaacggtgga ttgagcatgg ccgcgccgat gcggccggtc
     2221 ttgagcgcgg aaatcatcgc ggtttgtccc ggaatggtga tgacgttgac ctggctcgga
     2281 tcgattttcg actggctgag gatgcgccgc atgatgatgt cctgcaagcc gccgcgggaa
     2341 gagattgcaa ctattttgcc tttcaactcg gagatcgagc cgatattggc cgcgccgacg
     2401 agatcctgca ggggccggtc gacgaacagc atgacgatgc gcacgttgat gccgcggatg
     2461 gcggcggcga ctgcggaacc catggtcgcg gcgtattgaa tatcgccggc gagcaggact
     2521 ttgatgttga gtgatgccgg catgacgacc atctcggcgt tgaggccttc ctgttggaag
     2581 aagccatgct ctttggcgac gaagaacggc atggcggtga tggtcaccga cggcaggccg
     2641 atcttgatgt tttcggcgtg ggcaagagtc ccggcccata ggacggatga aagcaaaagc
     2701 ctgagcagca ttatcccctc gcttcagtcc agcggataca acatgcgggc gttgtcgttt
     2761 aagattttac gcttggtggc ggcgctgagg gttggatgat tgacgatcgg ttccgaggcg
     2821 ccgggccagc ggctgtcgaa gtgactgtag tcggaggcga agaccagcca ttcgtcgccg
     2881 aaagcgtcga tgacgtgggc gagctcgttt tcatcggcct cgaaggaaaa atagagctgg
     2941 ccgcgttcga gcaacttgct cggcggcgat ttcaattccg gcgtctgcac gcctaagact
     3001 tcataatgct cgtccatgcg gtgaaaccaa tagggcagcc agccgcagcc ggattcgagg
     3061 aatacgaagc gtaatttggg aaagcggtcg aggacgcctt cgccgaggat caccgacaat
     3121 gcgatcatct gctcgaacgg atggccgaac aggtgcgaat aaaaaaactt gtcgacccgt
     3181 tcggtgccgg tggcatcgga gccgtagcgg ccgagaaaca tatgcacgca gatcggcacg
     3241 tttaactttt ccgcttcggc gtagagcgga aaataataag gatcgccgag atggcgtcct
     3301 ttgacgttgg gaaaaatcga cgcgccgacc atgccaagtt gggtaacgca gcggcgcaat
     3361 tcgctcacgc tggcggcgat atcctgctgc gccagggcgg cggtgccttt gatgcgcttg
     3421 gggtctgcgg cgcaatactc tgccaaccag gtgttgcgcg ccgtggccag cgccacggcc
     3481 aagccggcgt cgtccaatgc cgggaccgtt cccgaaaggc cggcgccgaa aagcacggcg
     3541 acgtcgatct tctcgctatc catgtcgatc aggcgggcat ggggatcggt catgccggac
     3601 cggcgcgggt gcggccggct gtaggggccg ccgaccccga tgccaaggcc gctcggacgc
     3661 ggccaggctt tgtcttcgat tatggtgcgc agctggccgt taccgctgtc gcgccgttcc
     3721 ggcgcttggc tccgatactt ttccggcagc cgcgctttcc agtcgatgtg gttctcttcc
     3781 aagtggccgt cagcgtcgac ggtggcgaat gactctgcca tggggcctcg cttcagtggc
     3841 aattagagat tacaaattgc agatttctcg gcggctaggt gtcgagcaat ccggggaaaa
     3901 agacttcttc gatcttcgcc aggcgccggg ttaacccttc ctcgtgcgaa ttgcgaaaca
     3961 gggtttcgag catcttacga ttgttggcga tgccgaacgg gaagggatcg gcgccgacca
     4021 ttttttcctg gctggcgcga tcgctgtgct cgaaatagag gtaggcgggg ttgccgaaat
     4081 tgccgcgctg gtaggcgagg cttttgctct cgctgaacag gcggaaaatt tcctgagcta
     4141 gccagctgtt ctcttcgagc aggcggcgct gagcgacgat gacatggttc gccggcacga
     4201 tgccggtctt tttataaaac tggtcgacga tggacaagcc gccgtcagcg aacatcttgc
     4261 gcagccgcgg attgccttcc aacggcgtgc cgccgtagcg atcgatattc aaagtcatga
     4321 gtttagtatc gtgtcggggc gcgaagccgt aggcggcgtc gatttcgcct ttgtcgagca
     4381 tgacatcgaa ggtctgctcc ttggtgagcc aagtagtaga cacgtctgtg ggtgggcggc
     4441 tgtccatgcc gagtaacccg ccatggctta agtcttgaac ccggccgatg taccaggaga
     4501 tgtctttggg ctggataccg cagagctccc ggaggatgac tctgaaccac aagccggcgg
     4561 tcatcacgta gtcggggatg ccgatgcgtt tacctttgag atcgcagagc tcctgaatgc
     4621 cggcagcggt gttgacgaac agggtcatcc agacaaacgc cttggcgggg aagatcggta
     4681 gtgccgccca ctgccagcgg tcgcccttgg cctgctcgcg ggtgatgagg aattccgaaa
     4741 gcgacatttc gaagatatcg aattcgtcgt tcttgagatt tttaaagaac agctccggcg
     4801 gtgtggtgac gtgaatattt agattcgcct tgtccgattt gacgctgccg tcgatgagcg
     4861 gctcgaagcg gggatttttg gtgacaccga cttttagatt caacatggag tgactcgttt
     4921 cctaataatg atgttacctt catacgccga ggcgcgaggg aaggtcaaac agcgcgggcg
     4981 ccgccgccgc acagttgcag ccaaatggga aagcatgttc accacgaagt cacgaaggac
     5041 acgaaggttt cggataggtt caactccgaa cgtggcgcgc ttcgtccttc gtggtgagac
     5101 gaattcgtcc atcgccaaag acggtgagtc gtccgggtta cgttggattg cgcacgacga
     5161 aatgttgttc cggcacggga tagccggcgg cgccgcaggt ttcgcggatg actttgttgg
     5221 tatcgaaata aacctgccag taatgatcgg tgtggcagta gggacgtacg gcgagtaccg
     5281 gcccgttgaa gttgaattcg agcagctcga cgtccggcgc agggtcggtc ttgatattcg
     5341 ccaccttgcc gacccgttct ttcagcaatc tgatcgcttc gttgtggtcc accgaatggg
     5401 cgagttgcgc tttcaggtcg acgcggcgga acggattcgc cgagaagttc tgaatcgtgt
     5461 cggagaatat tttgctattt ccaagcatcg tcagcacatt atccggtgtc accagcgcgg
     5521 taacgaagag gccgatctcc ttgaccgtgc ccgtgacact cgcggcggtg acgaaatcgc
     5581 cgaccttgaa aggccgcaac acgatcatga acgcgccggc ggcaaagttg gccaacagtc
     5641 cgctccaggc caaaccgatg gcgacgccgg cggcggcgaa gagcgcggcg aaactggtgg
     5701 tctccacccc gaagtagcct aggatggcga cgaccaagat cacattcagt gtgaccgaga
     5761 tcacggtgcc gatgtaacgc agcaatgtgg gatcgactct ttgtttttcc agagccgatg
     5821 tcaccaggcg gatcgctaaa ctgatcagcc agcggccaat cagatagaaa atgatcgcgc
     5881 caatgatttt gaagccgatc agaataccga catcgactat cgtttgcatg gtcttgccta
     5941 tatccatgaa tgtaccctcc ttgagtcggg caacaatttt gtcgtgattg aaagtcttgc
     6001 gaagaatttg tcataggtta ggcgctgcac acttgtcaat gaaaaattgc cgcacgcagc
     6061 gtaaattcat atgcgcaccg catggactga ttccattaca ttcattggac attggacggt
     6121 gcttcagata tgagaggaaa tctcacatgg ctaaaaactc ggaggctcgg atatgaaaat
     6181 ctgtcattac gataataagc aggccggcgt cgtcgtcggc gaaaaagtta ttaacttcgg
     6241 cgacgcgatg atcaaagccg gtctggcgcg ccaaggctac accatgctgg aaatcatcga
     6301 cgctctggct aacaatccgg cggcgatgca aatcgcccgc gacgcggcga acggctcggg
     6361 cggtgtcgat ttaaaatcgg tgaaactgtt ggcgccgatc caaaatcccg gcacgctgtg
     6421 ggccgccgcg gcgaactacg gcgcgcaccg cgccgagatg gtcgatcgca tgggcagcgc
     6481 caacaagcag atcctcaaca aggacgactt gatggcggag tttttcatca agacctcgtc
     6541 gtcgatcatc ggccccggcg acacggtgat tttgccgaag atgtccaagc tggtcgactt
     6601 cgagtgcgaa ctgtgcgccg tcatcggcaa gaaggcgcgc aaggtcagcg aagcggattc
     6661 cctcgaccat gtgttcggct acatgatgtg ctgggacatc agccagcgcg atccctgggg
     6721 caagggcatg cacaacacgc gcaacatgcg caaaggcttc gatacgttta ccggtttggg
     6781 cccgtggatc gtgacgcgcg acgagatcgc cgagccgcag aatttgtcga ttacggtttt
     6841 gcagaacggc aaagaggcga tgatcgctca caccagcgac atgatctgcg gtctgcgcga
     6901 gcatattcgc tttctcagca actgtgtgac cctgcgcacc ggcgattgca tcaccacggg
     6961 cacgcccgcg ggcgtaagca aattgcacga tggcgacaaa ttatatggca ccatcgagaa
     7021 gatcggcagc atggaactca atgtgaaaga cgaagcatag attcttccag ccatcggcct
     7081 gacctctttg cgcgcgagca gaagtcggcg ttcagaatat cgaggagttg atttatgcca
     7141 gccgccccta aagccaatcc accgattcgt acattactcg gtcccggtcc aagtccggtg
     7201 agcccgcgtg tgctcgcggc actggcgctg ccggtggtcg gccatctcga tccgaagttt
     7261 ttggaaatca tggatcagag catggcgatg ttgcgcgagc tgtttcagac taacaaccgt
     7321 ttggcgctgc cgatgtcggg tacgggcagc gccggcatgg agacctgttt cgtcaatttg
     7381 atcgaacctc aggaccgcgt gctgatcggg gtcaacggcg tcttcggcac gcgcatggtc
     7441 gatgtcgcgc agcgttgcgg cgcgcaagtc gataccgtgg aggccgagtg gggcagcgcg
     7501 ctcgacgccc agaaggtcaa agaagcgctg gcgaaaaata aatacaaact ggtcgccgtg
     7561 gtgcatgccg aaacttcgac cggcgtgctc cagccgatgg acgacatcgc caaactggtt
     7621 cacgacagcg gcgcgctgtt gctggtcgat gccgtgacgt cattgggcgg tgcgccggtg
     7681 cgggtcgatg agctcggcat cgacgcctgc tacagcggca cgcaaaaatg tctgggctgc
     7741 cctccgggac tgtcaccggt aactttcaac gaccgcgctg tcgaggtaat tcgccagcgc
     7801 aagaccaagg tgcagagctg gtacctcgat ctgtcgatga ttgaaaaata ctggggcagc
     7861 gagcgggtct accatcacac cgcaccgatt tcgatgaact atgcgctgca tgaggcgctg
     7921 cgcgccgtcc tcgaagaggg gctggaaaat tcctggcagc gtcacaaaca gacccatgaa
     7981 gtgttcatcc gcgagatgcg caagctggaa atcgaaccgg cggtagccgc atcgatccgc
     8041 gcgccgatga tcaatgccat cacgatcccc gcaggcgccg acgacgccaa ggtgcgcacg
     8101 cgcttgtacg acgaatacaa catcgaaatc ggcgccggtc taggccctct caagggaaaa
     8161 atttggcgcg tcggtttgat gggccatggt tcgcgagtag agaatgtcga gactctcgcc
     8221 aaggcgctga agaaattaat ttaattcgaa ccaagcagat tcagtcgggt ccgaggattg
     8281 agatggatga ttgaggatcg ccgtcttcta tcctctagct tcgatcctcg acgaatcttt
     8341 tctagccata caacttcgcc acccactggc cgtagccgtt gtacggcagg gttggccggc
     8401 gttcgccgct gtcgatgagt ttttccgtcg cctgcgacca acgcgggtgc ggcgtgttgg
     8461 gatcgacgtt ggcggtgaaa gtgtattcgc ggggccggtt actatgccaa aacgttgccg
     8521 gctgctcggc ggtgaattcg atcttgacga ttgatttaat gcttttgaag ccgtacttcc
     8581 atggcacgac caggcgtagc ggcgcaccct gctgtttggg cagcggatga ccgtagatac
     8641 cggtggccat cagcgtgagt tcattcatcg cttccgccat cgtcaaccct tcggtgtacg
     8701 gccaggggcc ataggacagg gtttggttgg tcgcctggtc gggcttcaaa aacgtcacga
     8761 aacggacgaa cttggcggct gataacggtt cggctttctt gatcaatgct gcaagcggaa
     8821 atccgctcca cggcaccgcc atggcccagg cctcgacgca acgaaaacga tagagccgtt
     8881 cttcgagtcc catggcgcgc accagttcat cgatgtcgta aagccgcggc ttggcgacca
     8941 tgccggcaat ctcaacctgc catggatggg tcgtcaggcg cgcggcgttt ttgtaaaccc
     9001 gactcttgaa gacgctgaac tcgtaaaaat tgttgtacga ggcggcgtag gcttcgccgg
     9061 ttactggacg atcgagggtg aactttggat tgtggcgggc ggggtagaga tcggctcccg
     9121 ccggtcgctc ttgagcgccg acgcgaatgg cgtcgccggc agggccgcac gcggatatca
     9181 ggccgctggc gacgccgagt ccgatgagac ccgccgattt gaccagctgg cggcgctgac
     9241 ggaaaaatgt ttcgggtgtg agctcgcgct cggaaagttg ccaggatttt cgcttatcga
     9301 ccgccatgcg gtcatggtgc tgacgatgcc gccactttgc aagggcgggt ttaacgaaat
     9361 cgtgaaaccg acaagctagc ggtagaccgc gacgccgacg ggaatggtga attctttgcg
     9421 aatgtaacca ctcggtccat tgtggtgtga cttgacctcg aaccagtcgc ggctttggtt
     9481 aatcaccatg actttggtgc cgatggtgaa tgtcgcgagc actggtgcgc tgaagttcgg
     9541 ttccttgcgc agcaaggtcg cgtattt
//
LOCUS       DASQKS010000266         6865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_561935, whole genome shotgun sequence.
ACCESSION   DASQKS010000266 DASQKS010000000
VERSION     DASQKS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561935"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VE170_06940"
     CDS             <1..486
                     /locus_tag="VE170_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ15209.1"
                     /translation="VITGGEPLLAPDIADLTHELKRRGAHITIETAATIFKPVAGDLM
                     SLSPKLANSTPWKRSKGEFAVMHEKRRLNYPVMQQFLDGYDYQLKFVVDRKPDIDELR
                     AVIAQLQHVDASRVLLMAQGVTQAELKRKAEWIVESCKQYGFSYTPRLHIQLFGNRRG
                     T"
     gene            complement(522..3656)
                     /locus_tag="VE170_06945"
     CDS             complement(522..3656)
                     /locus_tag="VE170_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYJ15210.1"
                     /translation="MSGKTVPTELSPGPSLTGRLFYFLNFKRLKVWLWWLALLAIIVA
                     AFFVEVQTSLLQSWFFTSTNERLFYSLKDGLSREIIFPRPAAPFDERRGYAKLPLLQS
                     RLQSQGYLVTRQVRQSETMLTLLERGISPPYLERPDAGLEITGADGVRLFRYAQGDFL
                     FRKIDDIPPLLVKTLLFLENRDLDHPATSWQNPVIEWDRTFKAATMFLAAKLQLPVPV
                     QGGSTLAVQLEKFRHSPHGRTDNPVEKLRQLIGASLKAYQEGPNTRAWRERIIVDYLN
                     TVPLAAAPGYGEIHGLGEGLYAWFGIRLSDAVKDLQAAKPTPATLRAFKHSLALLISV
                     RAPSVFLVDERSSLEDKVSQFTRLMARDGVIDGDFAAQLQDTPIQFLPTAPLPPQPSS
                     GKNKAANAIRVNLLEALGMNNLYDLNRLHLRADSTIDVPLQKRVTDFFDRLTRPEVIK
                     AYGLNGERMLEADDPAKLIYSFLLVEATPLGNLVRVQADTLAAALDFNKSVKLELGST
                     AKLRTLTHYLELMAELHQEFSALTPKQLQERGSAARDPLTKWAAETLLDHKALQLQPF
                     LDNAMERKYSGSPYEGFFTGGGMHHFENFDKKENDRIYELRDAFRNSNNLALIRLMRD
                     LVSYHRARLAYDSNDLLDNADNPTRRQMLQEIAEEESRAALRRAYQNYAKQTPEEIVK
                     RMLGGKSSIERRLAVLFFAWRVGNDEDALTAWLEKHEAKTSAAEVGKLFRAYQNPRLT
                     LADHAYLLGMHPLDLWCAGEFRKNAKLSWKELYAKSGPAQQVGSAWLLNSRNQRAQNN
                     RLRIRIEKDAFARMTPYWQRLGFPFKTLVPTYATAIGSSSDRPTALAELVGILVNDGM
                     RRSTTTLTRIHFANATPYETLLERPLKTGDQVLVPEVARTVRKAMAEVVEQGTARRLS
                     GVFKLADGSVLTVGGKTGSGDNRFETFNRSGGVVSSRATNRTATFVFYLGDRYYGVIT
                     AYVQGRAAGNYHFTSALPVTLLKMLAPSITAKFDNKQLDEIPPPVGETPVEITAQPTN
                     LAPTSQSKPHPSSL"
     gene            complement(4230..4661)
                     /locus_tag="VE170_06950"
     CDS             complement(4230..4661)
                     /locus_tag="VE170_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15211.1"
                     /translation="MKVLDVLETSKGRKKGQRRIDLFRRENLETWILCFPPGDVQVMH
                     NHPSDRSYYVLTGKGIIKGRDETHEVSQGQIISIPAHEFYEGSNPNDEPMILLGNSHK
                     ATAEDVAKAGGQRNEIDAKTGKRVIFQHGGGQDMGVLADWN"
     gene            4926..5822
                     /locus_tag="VE170_06955"
     CDS             4926..5822
                     /locus_tag="VE170_06955"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15212.1"
                     /translation="MALSKLNCMYGHLEGIDGKFARDVTGYLSIADGIYEKHGLEISW
                     NHVQGTEERYRRLGNGEAQLSFVVGRASLQHFLDTKTTRVIGCAMNSCPYLLVADAAI
                     KVIKDLKGQTVVCRDGPARGVPLGRQLQQLGGLATDDYQITLVASDQEAFHSLLDKKA
                     AAAIVPRPYGFVAEDKGFHRMLDWNDLVDDPLPITIETTQKLFGEREKDFRTFLTAHR
                     EGILHAKANREKVVQLLIDKFAHSRSLAERTFDEYLGYMDPSLQIDMLHMGKLVAQVS
                     TEQAANARQIASEWAIPGAVKA"
     gene            6042..>6865
                     /locus_tag="VE170_06960"
     CDS             6042..>6865
                     /locus_tag="VE170_06960"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15213.1"
                     /translation="MVYPVTAAEVTIALPAKSFQQIIFPLAQDRGYMKEEGIDLKITF
                     MEPTPSIQALVADSIQVTGSGGSALIAIARGNVPMKVVLAVNDRVHQWILSRPNITSI
                     KDLKGKKIATTGVASISTFMFRNLLPKHGLDGSKDVFFVDPGVGNQAVALMSGAVDAA
                     IVSVEQRYAGVDNGMKELMYLGNEVKNSWGTTAASEKFMKDQPKVMAGFMRATLKALR
                     LIKRDRDGTIAAFTKFSALPQTTVTRLYDDLIGSFTANGAVDEDTQRNDLAVIRQVL"
BASE COUNT         1512 a         1834 c         2044 g         1475 t
ORIGIN      
        1 gtgattaccg gcggtgaacc gctgttggcg cccgacattg ccgacctaac acatgagctc
       61 aaacggcgcg gcgcccatat caccatcgaa accgcggcga cgatcttcaa accggtggcc
      121 ggcgatttga tgtcgttgag cccgaagctc gccaactcga cgccatggaa aagaagcaag
      181 ggtgagttcg cggtcatgca tgaaaagcgc cggctcaatt atccggtcat gcagcaattt
      241 ctcgacggct acgactacca actcaaattt gtcgttgatc ggaaaccgga catcgacgaa
      301 ctccgcgccg tcatcgcgca gttacaacac gtcgatgcga gccgagtcct actcatggcc
      361 cagggcgtca cacaagcgga gttaaagcgc aaagccgaat ggatcgtcga atcgtgcaaa
      421 cagtatggct tcagttatac gccgcgtttg catatccagc tgtttggcaa ccggcgcggc
      481 acgtgatgcc gcgcgccccg cggcattacg tgagcctatt tttacagact actgggatgc
      541 ggcttgcttt gcgaggttgg ggcaaggttg gtcggctggg cagtgatctc gactggggtt
      601 tcacccaccg gcggcggtat ttcgtcgagt tgcttgttat cgaatttcgc cgttatcgag
      661 ggggcgagca ttttgagtaa agtgaccggt agcgcgctgg taaaatgata gttgcccgcc
      721 gcgcgtccct ggacgtacgc ggtgataacg ccatagtaac gatcgccgag gtaaaatacg
      781 aaagttgccg tgcgattggt agcgcgtgag ctgacaacgc cgccgctacg gttgaaggtc
      841 tcaaatcgat tgtcgcctga gccggtcttg ccgccgacgg tgagcacgct gccgtcggca
      901 agtttgaata cgccgctgag ccggcgcgcg gttccctgtt cgacaacttc ggccatggct
      961 ttgcgcacgg tgcgagcgac ctcggggacg agcacctgat cgccagtttt cagcggccgt
     1021 tcgagcagag tctcgtaggg ggttgcattg gcgaaatgaa ttctagtcag tgtcgtggtt
     1081 gatcggcgca taccgtcgtt gaccaaaatg ccgaccaatt ccgccaatgc cgttggccgg
     1141 tcggaagaac tgccgatggc ggtggcgtag gtcggtacca gagttttgaa tgggaaaccg
     1201 agtcgctgcc agtatggcgt catgcgcgcg aaagcatctt tttcgatgcg gatgcgcaga
     1261 cgattgtttt gcgcccgttg attgcgcgag ttaagcagcc aggccgagcc gacctgctgg
     1321 gccggaccgc tcttggcata aagttccttc cacgacaact tggcgttctt gcgaaattcg
     1381 ccggcgcacc acaaatcgag cgggtgcatg ccgagcagat aggcatggtc ggccaaggtc
     1441 agacgcggat tttggtaggc tcgaaacaat ttgcccactt cggcggcgct ggttttggcc
     1501 tcatgctttt ccagccaagc ggtcagggcg tcttcgtcat tgccgacccg ccaggcgaaa
     1561 aacagaacgg ccaggcgccg ctcaatgctg cttttgccgc cgagcatgcg cttgacgatc
     1621 tcttccggtg tctgcttggc gtagttctgg taggcgcggc gcaacgcggc gcgggattcc
     1681 tcctcggcga tttcctgcag catctggcgg cgcgtcgggt tatcggcatt gtcgagcagg
     1741 tcattggaat cgtaagcgag ccgtgcgcga tggtaactga cgagatcgcg catgaggcga
     1801 atgagcgcga gattgttcga gttgcgaaag gcatcgcgaa gttcgtagat gcggtcattt
     1861 tcctttttgt cgaaattttc aaagtggtgc attccgccgc cggtgaaaaa gccttcatag
     1921 gggctcccgg aatatttccg ttccatggca ttgtcgagaa acggttggag ctgcagtgct
     1981 ttatggtcga gcaaggtttc cgccgcccat ttggtcaatg gatcgcgcgc cgcgctgcca
     2041 cgctcttgca gttgtttcgg ggtcagtgcc gaaaattctt ggtgcagctc cgccatcagt
     2101 tcgagataat gggttaacgt gcgcagtttc gctgtgctgc cgagttcgag tttgacgctt
     2161 ttgttgaaat ccaaagctgc ggcgagggtg tcggcctgca cccgcaccaa attgccaagg
     2221 ggcgtcgctt cgacaagtag gaatgaatag atcagcttcg ccggatcgtc ggcttcgagc
     2281 atgcgctcac cgttgagacc ataggcttta atcacctcgg gccgggtcaa acggtcgaag
     2341 aagtcagtca cgcgcttctg cagcggtaca tcgatggtgc tatcagcgcg caggtgtaag
     2401 cgattcaagt cgtaaagatt gttcatgccc agcgcttcga gcaagttgac gcggatggca
     2461 ttagccgcct tgtttttacc ggaggatggc tgcggcggta gcggtgcggt gggcaagaac
     2521 tggatcggtg tatcttgcag ttgggcagcg aaatccccat cgatcacacc gtcgcgcgcc
     2581 atcaggcggg tgaactggct aactttatct tccaaggatg accgttcgtc gaccaggaag
     2641 accgacggcg cgcgcacgga aatcaacaga gcgagagaat gcttgaaggc gcgcagtgta
     2701 gccggcgtgg gcttggcggc ttgcaaatcc ttgaccgcat cgctcaagcg gatgccgaac
     2761 caggcgtaca gcccttcgcc caggccgtgg atctcaccgt agccgggggc ggcggctagc
     2821 ggcacggtat tcaaatagtc tacgataatc cgttcgcgcc aggcgcgggt gttgggtcct
     2881 tcttgatacg ctttcaagct ggcgccgatg agttggcgca acttttcaac tgggttgtca
     2941 gtccggccat ggggagaatg gcgaaatttc tcgagttgca ccgccaaagt gctgccgcct
     3001 tgcaccggca ccggcagttg tagtttggcg gcgagaaaca tcgtcgcagc tttgaatgtc
     3061 cggtcccact cgatgaccgg gttctgccac gaggttgccg gatggtcgag atcgcggttt
     3121 tccaggaaca atagcgtctt gaccaagagc ggcggaatgt cgtcgatttt acggaataaa
     3181 aaatccccct gagcgtaacg gaacagccgc acgccatcgg cgccggtgat ttccaaaccg
     3241 gcatctggcc gttccaagta gggcggcgag atgccgcgtt ccaagagcgt caacatggtc
     3301 tcggactggc gcacttgacg cgtaaccaaa tatccctggg attgcagccg gctttggagc
     3361 agcggcaatt tcgcatagcc gcgccgctcg tcaaaaggcg cggcggggcg tgggaaaatg
     3421 atctcccggc ttaggccgtc tttaagacta taaaatagcc gctcgttagt gctggtaaaa
     3481 aaccaagatt gcagcagcga ggtctgaacc tcgacaaaaa acgcggcgac aatgatcgcc
     3541 agaagcgcca accaccaaag ccagactttt agccgcttga aattcaggaa gtagaacagt
     3601 cgcccggtga gggatggccc cggcgacagc tcggtaggaa cagtttttcc gctcatcgaa
     3661 gtgtggagcg atattaacac accggtggca aaaaactaaa tgaagtttcc cggtctaatg
     3721 cctccataaa gcacttcgaa gccggaaaga actcgcgcca ataacgccat ggcaagcacg
     3781 gagatctgcc taagcaaatc aaaaatggtg aaaaaccaac aacttaaatg cgccaaacaa
     3841 aaaaggatcg aactgcgggt ttgggttttc cgggttggaa gtcatgtacc ccaatttgcc
     3901 gagaagggct tggggaacga ggatttctct cggcttacct cgccgcatag ccgcgccgcg
     3961 aggttgggac agtgccgatg gcgacaggct ccctcgaccg cgccttcagt cccgagcttg
     4021 tcgagggatt gcctaaggat cgtcgacgat tactgttaac cttccatgca accctgcggc
     4081 acagccatgg cagagctgca gataataaag gttaacaaga atgaatgatg gtatccgcaa
     4141 taaacctacg aggcgcggtg atgttccagt tcgaaaaaaa gcccggcgag ttggcactcg
     4201 accgggcttt gtcgttgttg atgattaaat taattccaat cggcgagcac gcccatgtcc
     4261 tggccgccgc catgttggaa gattacccgc ttgccggtct tggcgtcgat ctcatttctt
     4321 tggccaccgg ctttggcgac gtcttccgcc gtcgctttgt gactattgcc taacaagatc
     4381 atcggctcgt cattgggatt ggagccttcg tagaactcat gggccgggat gctgatgatc
     4441 tgcccctggg aaacctcgtg ggtttcgtca cgccctttga taatgccctt gccggtcaga
     4501 acatagtagc tccgatcgga cgggtggttg tgcatcactt gcacgtcgcc gggagggaag
     4561 caaagaatcc aggtttccaa attctcacgg cgaaaaagat cgatgcgccg ctggcctttc
     4621 ttgcggccct tcgacgtttc caaaacatcc aatactttca tgggaccctc ctgtggttgg
     4681 tgttattgcg acccaatgga agaatttagt ccggttattg gagcactgtc aaggcgaagc
     4741 aatggccttg ttagattgcc ggccagatgc acggcgcgaa acagatttcc gcttctttcg
     4801 aaaagacggt agcgctgatc atcgcgaacg gcgagaggta cggataaaac cgaatcgtcg
     4861 gtgcaagtga agtttgtctg ccggcgccaa ccgtaatata gagaaaggcg aaggagaaac
     4921 ccctaatggc tctgagcaaa cttaactgta tgtacggaca tctcgaaggc atcgacggca
     4981 agttcgcccg cgatgtcacc ggttatctgt caatcgccga cggcatctat gaaaaacatg
     5041 gactggaaat ttcctggaat cacgtgcagg gaaccgagga gcgttaccgg aggttgggaa
     5101 atggcgaagc gcagctgtca tttgtcgtcg gccgcgcgtc gttgcagcat ttcctcgata
     5161 ccaagactac ccgggtgatc ggctgcgcca tgaacagttg tccgtatcta ttggttgccg
     5221 acgcggcgat caaagtgatt aaggatctca aaggccaaac cgtcgtttgc cgcgatgggc
     5281 cggcgcgcgg tgtgccgctg gggcggcagt tgcaacagct gggcggcctg gccacggatg
     5341 attaccagat cacgctggtc gccagcgacc aagaggcgtt ccacagcttg ctcgacaaaa
     5401 aagccgcggc ggcgatcgtg ccccggccct atggtttcgt cgccgaagat aaaggttttc
     5461 accggatgct cgactggaac gatttggtcg acgatccgct gccgatcacc atcgaaacga
     5521 cgcagaagtt gttcggcgag cgcgagaagg atttcagaac tttcttaacg gcccaccggg
     5581 aagggattct ccacgccaag gccaatcgtg agaaagtcgt ccagctattg atagacaaat
     5641 tcgctcactc tcgttccctg gctgagagga cgtttgacga gtatctcggc tacatggacc
     5701 cgtcgctgca gatcgacatg ctgcacatgg gcaaactggt cgcccaagtg tcgacggaac
     5761 aggcggccaa tgcgcgacag attgcgtccg agtgggcgat acctggagcg gtaaaggctt
     5821 agcccggagc ggcgggttca gctcaacgtt aagcagttca aaatgagaac cgatgccgtg
     5881 cctatacacc cccaaaagta tttattgcga ttcgcatgga atcggtcgag gataaggagc
     5941 ttgctctcat gttcattcgc gcactagcac gcatacatat ttcgccgacg gtcgtgtgcg
     6001 taacgatgtt ggttgcccta ctttgcaatg cgataacgca aatggtgtac ccggttaccg
     6061 ccgcggaagt caccatcgcg ctgccggcca agagctttca acagattata ttcccgctgg
     6121 cacaggaccg cggctacatg aaggaagaag ggatcgactt gaagattacc ttcatggagc
     6181 cgacgccgag catccaggcg ctggtcgccg atagtatcca ggtgacgggt tccggcggca
     6241 gcgcgttgat cgccatcgcg cgcggcaatg tgccgatgaa agttgttctc gcggtgaatg
     6301 atcgagtgca ccagtggata ctcagccggc cgaacattac cagcatcaag gatctcaaag
     6361 gcaagaagat cgccaccacg ggggtcgctt ccatctcgac ctttatgttt agaaatctcc
     6421 tgcccaagca tggcctcgac ggcagcaaag atgtgttctt tgtcgatcct ggtgtcggca
     6481 atcaagcggt ggcgctgatg tcgggcgccg tggatgcggc gatcgtcagt gtcgagcagc
     6541 gttatgcggg ggttgataac ggcatgaagg aactcatgta tctcggcaac gaggtgaaaa
     6601 actcctgggg caccaccgcg gcttcggaga aattcatgaa ggatcagccc aaggtcatgg
     6661 ccgggttcat gcgcgcgacg ttgaaagcct tgcggctgat caaacgggat cgcgatggca
     6721 ccatcgccgc gtttaccaag ttttctgcct tgccccagac gaccgtaacg cggttgtacg
     6781 acgacctgat cggcagcttt accgccaatg gcgccgtcga tgaagacacg cagaggaacg
     6841 atctcgcggt gatccgtcaa gtgct
//
LOCUS       DASQKS010000267         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6032210, whole genome shotgun sequence.
ACCESSION   DASQKS010000267 DASQKS010000000
VERSION     DASQKS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6032210"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..717)
                     /locus_tag="VE170_06965"
     CDS             complement(286..717)
                     /locus_tag="VE170_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HYJ15214.1"
                     /translation="MSKDERGRATRRGQPYLEEPDAKPFWANLREHKLTAQRCSSCHK
                     INGFPPQSHCRYCLANEYEWVPLSGKGTVYSHIAYHRAWHPGYQNKVPYNVSLVDLAE
                     GGRLVTNVVNCPPEEVKIGMNVEVIFEDLPDYTLPKFQPVK"
     gene            complement(714..1868)
                     /locus_tag="VE170_06970"
     CDS             complement(714..1868)
                     /locus_tag="VE170_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYJ15215.1"
                     /translation="MTNLSGKCAIVGMGETVVGKRPDATTNSLHLEAIKACLDDAGIK
                     ASQVDGLLTNQPLNDAHRSYATKLSHMAGIDPLFATDLALGGATPIAMVQHAVMAIEA
                     GMATTVVCVHARKRSTADPTPGHPIRRGDEQWEEPWGHFAAAGGHAFAAQRHMYEFGT
                     TSEDLAHVAVSTRYHASLNKNATLRKPMTIDEHQQSRMIVAPLRLFDCSLESDGGGAV
                     LVTSAERARDFPKKAVSILGMGQHHPHFSLLDAPTLTTLGGKKSSETAYRMAGMKPKD
                     MQFAQIYDCFTITAMITLEDYGFCEKGEGKDFVKNGRITLGGQLPLNTHGGLLSQAHL
                     EGQLHITEAVKQLRGNEVEPERQVANAKVGIVSGHGGSLAMHATMILGVP"
     gene            2223..3191
                     /locus_tag="VE170_06975"
     CDS             2223..3191
                     /locus_tag="VE170_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15216.1"
                     /translation="MLSLSKRTLLIGFVWLLGLPHGTQARDAVIGYSGRTIAEMPFAW
                     ASRKGFFKEANLDVKMIYMSTSIAAKGLMSGDVDYSAAMGGTLRAAIAGAPVIGIYSM
                     FKAQMYLVSQAKYKQVADLKGKTVGISVFGGAYDLAARTILKHHGLDAEKQVTLLQIG
                     DSRTLYSALSAGTIDSAILGPPYDIKAELEGANRLFDSSEIFDMPFSGLATSKKKLAQ
                     DRAEVKAIVRGLERIRRYIAANRSEAEGFAKQFLNLNDKEARLSIAQMVKSFRDTGRT
                     TDEAVVDFIETTLRSTKQKAGAIKPSDIVDWSVTTEVVRELDKEKK"
     gene            3288..4403
                     /locus_tag="VE170_06980"
     CDS             3288..4403
                     /locus_tag="VE170_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15217.1"
                     /translation="MIIDADGHTHVSNLVFDEYLDPAFRAKRPRYVELDDGRGVYIIE
                     GRVIIKPSGWGPGTPLSIGSRRLEGCQSFGMENIAGRVADLDREGIDVQVIYPNVLMS
                     INSFDDAALATAVARAYNRWMSDRCAESEGRLRYAAVVALQDPLAAADEVEYAATKLG
                     AVGVMVPGTVGTRTLDHPDLYPFWQRVEKCDVGVGIHTVTGMYPTVGADLFDHFFGSK
                     AVAMPLTLTVALVSLIAGGIADLFPKLRFGLLETGCGWLPYWLERLDDLHERGEHEPA
                     IYDGLLPRQVSSKRTKPSDLFTSGRMIVSCEPGEKTLPYVVSAVGDQCIMYASDYPHP
                     DSKWPNTVAPIQQAQMSASAKQQILGVNAARLYKLGE"
BASE COUNT          901 a         1290 c         1359 g          969 t
ORIGIN      
        1 gaaagtaagc ctgcgtcaaa ggaattcata aatccagaat gatttccacc cgagtgctgg
       61 caacaagaaa gatttctcgc tgcgctcgaa atgacagatc gcgagttgtc atcccgaaca
      121 aagagaggga tctagaccag ctcaaccagc attgaggatg ctgaaaatct ctctccgagt
      181 ccttcgacga tgctcaggac gaaaggaggg gactggtgtc cttttggatc ttccgttcat
      241 gccgtggctc tcgatgcgtt ccgctttttc tgcaatctac tcggctcatt tcaccggctg
      301 aaacttcggc agcgtatagt ccggcagatc ctcgaagatc acttcgacgt tcatgccgat
      361 cttaacttct tccggggggc agttgaccac gttggtcact agacgcccac cctcagccag
      421 gtcgacgagg gacacgttgt agggaacctt attttggtac ccaggatgcc aagcccggtg
      481 gtaggcgata tgagaataga ctgttccttt gccgctgagc ggtacccact catactcgtt
      541 cgccaggcaa taccggcaat gggactgcgg cgggaagcca tttattttat ggcacgatga
      601 acagcgctgg gcggttagtt tgtgttctcg caaattggcc caaaagggtt tggcgtcggg
      661 ctcttcgaga tagggttgtc ctcggcgcgt tgcgcgcccg cgttcgtctt tgctcatggt
      721 acccccagga tcatagttgc atgcatcgcc agactgccgc catggccgct gacgatgccg
      781 actttggcgt ttgccacctg ccgttcgggc tcgacttcgt tgccgcgcag ttgtttcacc
      841 gcctcggtga tgtgcagctg gccttcaaga tgggcttgcg ataacaagcc gccatgagta
      901 ttgagcggca gttggccgcc gagagtaatc cgtccgttct tgacgaagtc tttgccttcg
      961 cccttctcgc aaaagccgta gtcttccaag gtgatcatcg ccgtgatcgt gaaacaatcg
     1021 tagatctgcg cgaactgcat gtctttaggt ttcatccccg ccatgcggta agccgtctca
     1081 gacgatttct tgccgccgag agtcgtcagg gtcggcgcat cgagaaggct gaaatgcgga
     1141 tgatgctgac ccatgcccaa gatcgacacc gctttcttgg ggaaatcccg cgcccgctcg
     1201 gcgctggtca ccaataccgc gccgccgcca tcggattcca gagagcaatc gaacaagcgc
     1261 agcggcgcga cgatcatgcg cgactgctgg tgctcgtcga tggtcatggg tttgcgcaag
     1321 gtggcgttct tgttgaggct cgcatggtaa cgcgtcgaca ccgccacgtg ggcgagatct
     1381 tcgctggtgg tgccgaattc atacatgtgt cgctgcgccg caaaggcgtg gccgccggcg
     1441 gcggcaaagt gcccccaggg ctcctcccac tgttcgtcgc cgcggcgaat cggatgaccg
     1501 ggcgtcggat cggcggtcga gcgcttgcgc gcatggacgc agacgacggt ggtcgccata
     1561 ccggcttcga tcgccatcac tgcatgctgc accatggcga tgggcgtcgc gccgccgagc
     1621 gccaaatcgg tggcgaacag cggatcgatg ccggccatat gcgagagctt ggtggcgtaa
     1681 ctgcggtgcg cgtcgttgag cggctggttg gtaagcaggc cgtcgacctg ggaagctttg
     1741 atgccagcgt cgtccaaaca ggctttgatc gcttcgaggt ggagtgaatt agtggtggcg
     1801 tcgggacgtt ttcccaccac cgtctcaccc atgcccacga tggcgcattt gccactgagg
     1861 ttcgtcatgt ttttcctttc gtcgactctt taaaaggcga acacagccat gaaccgatca
     1921 cgtgtttttc ggtccgaagg ccaacttctt gttggagcga tcttatacgg taggtttttc
     1981 cactgtcaag gcgatgtgtg gaatcatgca atggttcaat cgcgcgctag ttctcgcgtc
     2041 ggtgatttgg tttgcagtcc ggtcaggccg tttgctaaat gagtggagct cgggaacgcg
     2101 cttcgacaag ctccgcatga acggcagttt ttttacgggt caggtccctt gccgatcgcc
     2161 ctgggctcat cgaagaattc cgagaaggtt tattcggcaa cttgttacgt attggaggtg
     2221 tcatgctttc gttgtcaaag agaacgctcc taatcggttt tgtctggtta ctcgggttac
     2281 cccatggcac tcaggcgcgc gacgcggtca tcggctactc cgggcggact atcgccgaga
     2341 tgccgtttgc ctgggcgagc cgcaagggtt ttttcaaaga agccaacctg gatgtgaaaa
     2401 tgatttatat gtcgaccagc atcgccgcca aggggttgat gagcggcgac gtcgattact
     2461 ccgccgccat gggcggcacg ctgcgcgcgg ccatcgccgg cgcgccggtg atcggcatct
     2521 attccatgtt caaagcgcag atgtacttag tttcccaggc caaatacaaa caggtcgccg
     2581 atttgaaggg caagacggtc ggcatcagcg tgttcggcgg cgcctatgat ctcgccgcgc
     2641 gaacgattct caaacatcat ggtctcgatg ccgaaaagca ggtgacgctt ttgcagatcg
     2701 gcgacagccg cactctatac agcgcactca gcgccggcac catcgacagc gcgatcttgg
     2761 ggccgcccta tgacatcaag gccgagctgg aaggcgccaa ccgcctgttc gattcgtcgg
     2821 agattttcga tatgccgttc tccggcttgg cgacgagcaa gaaaaaactg gcgcaagacc
     2881 gcgccgaggt gaaggcgatc gtgcgcggcc tggaacgcat ccgccgctac atcgccgcaa
     2941 accgcagcga ggctgagggg ttcgccaagc agttccttaa tttaaatgac aaggaggcgc
     3001 gcctttcgat cgcgcagatg gtgaaatcct tccgcgacac cggccggacc accgacgaag
     3061 cggtggtgga tttcatcgag accacgttgc gcagcacgaa acagaaagcc ggggcgatca
     3121 agccgagcga tatcgtcgac tggagcgtga cgacggaagt cgtgcgggaa ctcgataaag
     3181 agaagaagta agggtgaagg gtaaggcgca aggcgagtcc ggacactcat ctcgccttac
     3241 ccttcacgct tatccccttg cgatatcgcg atgagaggga gcgaacaatg ataatcgacg
     3301 ccgatggcca tacgcatgtc tctaatctcg tgttcgacga atatctcgat ccggcgtttc
     3361 gcgccaagcg gccgcgttat gtcgaactcg acgacggccg cggcgtttac atcatagaag
     3421 gccgggtgat catcaaaccg tccggctggg ggccggggac gccgctgagc atcggcagcc
     3481 gccggctcga aggttgccag tcttttggca tggaaaacat cgccggccgc gtggctgacc
     3541 tcgaccgcga aggtatcgac gtgcaagtga tctatcccaa cgtcttgatg tcgatcaaca
     3601 gtttcgacga tgccgcactc gcgacggcgg tggcgcgggc atacaaccgc tggatgtcgg
     3661 accgctgcgc cgagtcggaa gggcgcttgc gttatgcggc ggtggtggcg ttgcaagatc
     3721 cgctggcggc tgccgatgaa gttgaatacg ccgcgaccaa actgggcgca gtcggcgtca
     3781 tggtgccagg caccgtcggc acgcgcaccc tcgatcatcc cgatctctac ccgttttggc
     3841 agagagtcga gaaatgcgat gtcggcgtcg gcatccacac cgtcacgggc atgtacccga
     3901 cggtaggcgc ggatttattc gatcacttct tcggctcgaa ggcagtcgcc atgcctctga
     3961 ccctgacggt ggcgctggtg tcgctcatcg ccggcggcat cgccgatctg tttcccaagc
     4021 tgcgcttcgg tttgctggaa acaggctgcg gctggctgcc ctactggctc gaacggcttg
     4081 acgatctgca cgaacgcggc gaacacgagc cggcgatcta cgacggcctg ctgccacggc
     4141 aagtctcttc gaagagaacc aagcccagcg atttatttac ctcgggccgc atgatcgttt
     4201 cctgcgagcc cggtgagaaa accttgccct acgtcgtcag cgccgtcggc gaccaatgca
     4261 tcatgtacgc ctccgactac cctcatcccg acagcaagtg gcccaacacc gtcgcgccga
     4321 ttcagcaggc gcagatgtcc gccagcgcca agcagcagat ccttggggtg aatgccgcgc
     4381 gattgtataa gttgggagag taaccgagcc gagaacaatg caggtgcgtt taaggcttcg
     4441 tgccggtttt attttttcgc cgcgagttct ttggcggcgg cggcggacag tgaaaaatca
     4501 aagatcgtcg ccgccggca
//
LOCUS       DASQKS010000268         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6032719, whole genome shotgun sequence.
ACCESSION   DASQKS010000268 DASQKS010000000
VERSION     DASQKS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6032719"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /gene="urtB"
                     /locus_tag="VE170_06985"
     CDS             <1..1384
                     /gene="urtB"
                     /locus_tag="VE170_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HYJ15218.1"
                     /translation="IAGSGGTKDALTGAPVDAPAAQHEAIMINNRIRAALDGAIAALK
                     LVSPDRAVRLAAARAVAGDPSAEMLPLIKTALDKETDNEVKRLLNQARALIEINSGDA
                     ALRREAAKLLGSSGNPQAKQIIGKLLEKHPDGSFIEADAGVREEAIKALRAIERGSLM
                     PQIAATLFSGLSLGSVLLLAALGLAITFGLMGIINMAHGEMLMLGAYTTYVAQSLFRA
                     YLPDWLDWYIAAAVPMAFAVAASVGIALERTVIRFLYGRPLETLLTTWGISLMLTQLV
                     RTVFGAQNVEVANPAWMSGGFSLGADVVLPYNRVVIIVFAAFVVFLVWLFLARTRLGL
                     YVRAVTQNRTMADCMGIPTPRVDWLTFGLGSGIAGLGGVALSQIGNVGPDLGQSYIVD
                     SFMVVVLGGVGQLAGTIIAAVGLGEINKFLEPFTGAVLGKIFVLIIIILFIQKRPQGL
                     FALKGRMAES"
     gene            1386..2477
                     /gene="urtC"
                     /locus_tag="VE170_06990"
     CDS             1386..2477
                     /gene="urtC"
                     /locus_tag="VE170_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HYJ15219.1"
                     /translation="MNSQVTLANSFYSRPAWFGLGVFSAAVLIVVPALRLLLPAENPL
                     HLSDYYILMIGKIMCYAIVALAMDLIWGYAGILSLGHGLFFALGGYSMGMYLMREIGN
                     EGQYRSFLPDYMVFLDWKEFPWYWAGTEYFSLALVKILLVPGLLALVFGFFAFRSRIK
                     GVYFSIITQALTFAFMLLFFRNDTGFGGNNGFTDFKRILGFSLAHPGTRVALFAITGA
                     VLILTLLLCRYIVTSKFGRVLTAIRDAESRTMFSGYNTRDYKLFVWTLSAALCGIAGA
                     LYVPQVGIINPSEMSPANSIEIAIWVAVGGRGTLAGAILGAAIVNGAKSYFTVAWPEF
                     WLYFLGALFVVVTVYLPNGVMGLTRRGNP"
     gene            2489..>3169
                     /gene="urtD"
                     /locus_tag="VE170_06995"
     CDS             2489..>3169
                     /gene="urtD"
                     /locus_tag="VE170_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HYJ15220.1"
                     /translation="MGLDGGIESTGISHVVQAEAIDVSHKAILYLEGVTVSFDGFRAL
                     NDLSFSIDVGELRCVIGPNGAGKTTMMDVITGKTRPDTGTVFLGQTIDLTALPEYEIA
                     RLGVGRKFQKPSVFENHSVYENLALAIKSPKGVWNTLFHRRDSAQEDRIDAMLQQIHL
                     KERMRDQAGLLSHGQKQWLEIGMLLMQEPQVLLLDEPAAGMSDEETERTAELFLSLEG
                     KHSLIVVEH"
BASE COUNT          584 a          910 c          925 g          750 t
ORIGIN      
        1 aatcgccggc agcggcggta caaaggacgc gctgaccggt gcgccagtgg atgcgcccgc
       61 cgctcaacac gaggcgatta tgatcaacaa tcgcattcgc gcggctctcg acggcgccat
      121 tgccgcgctc aaattggttt ctcccgatcg cgccgtgcgt ttggccgccg cgcgtgcggt
      181 ggccggcgat cccagcgccg aaatgctgcc gttaatcaaa accgctctcg ataaagagac
      241 cgacaacgaa gtcaagcgtc tgcttaatca ggctcgagcg ctgatcgaga tcaacagcgg
      301 cgatgcggca ctgcgccgcg aggcggcgaa attattgggc agcagcggta atccccaagc
      361 gaaacagatc atcggcaaac tgctggaaaa acaccccgac ggtagtttta tcgaggccga
      421 cgccggtgtg cgcgaggagg cgatcaaggc attgcgcgcc atcgaacggg gctcgctgat
      481 gccgcagatc gccgccacct tgttcagcgg tttgagcctc ggcagcgtcc tgctgctggc
      541 ggcattggga ttggcgatca ccttcggttt gatgggcatc atcaacatgg ctcacggcga
      601 aatgctcatg ctcggcgctt acaccacgta tgtcgcccaa agtttgttcc gcgcctatct
      661 tcccgattgg cttgattggt acatcgctgc tgcggtgcca atggcgtttg cggtggcggc
      721 gtcggttggc atcgcgctcg agcgcaccgt cattcggttc ctctacggcc ggccgctgga
      781 aaccttactc accacttggg gtatcagcct gatgctgacc cagttggtgc gcacggtttt
      841 cggtgcgcaa aatgtcgaag tcgccaatcc ggcctggatg tcgggtggat tcagtctcgg
      901 cgccgatgtc gtgctgcctt ataaccgcgt cgtcataatt gtctttgccg cctttgtggt
      961 atttctcgtc tggctgtttc tggcgcgcac ccggttaggt ctttacgtac gcgccgtgac
     1021 gcagaatcgc accatggccg actgcatggg catcccgacg ccacgagtcg actggctcac
     1081 tttcggcctc ggatccggca tcgccgggct gggtggcgtg gccttatccc agatcggcaa
     1141 tgttggcccg gacttgggcc aaagttatat cgtcgactcc ttcatggtcg tggttctggg
     1201 cggcgtcggc caattggccg gcaccatcat cgccgccgtc ggtctgggcg aaatcaataa
     1261 atttttggaa ccgttcaccg gcgccgtgct cggtaagatt tttgtcttga tcatcatcat
     1321 cctgttcatt cagaaacgac cccaagggtt attcgccttg aagggccgca tggcggagag
     1381 ctgaaatgaa ttcgcaggtg acgctagcga attcatttta cagccgcccg gcttggttcg
     1441 gcctcggggt attttccgcc gccgtgctga tcgtcgttcc tgccctgcgc ttgcttctgc
     1501 cggcggaaaa cccgctgcac ctttccgact attacatcct catgatcggc aagatcatgt
     1561 gctacgccat cgttgccttg gccatggacc tgatctgggg ttacgccggt atcctctccc
     1621 tcgggcacgg cttgttcttc gccttgggtg gttactcgat gggcatgtat ctcatgcgcg
     1681 agatcggcaa cgaaggccaa taccgaagct tcttgccgga ttacatggtg ttcctcgact
     1741 ggaaagagtt tccctggtac tgggccggca cggaatactt ttccttagcg ttagtaaaaa
     1801 tcttgctggt gccgggattg ctcgccttgg tgtttggttt tttcgccttt cgttcgcgca
     1861 tcaagggcgt ctatttttcg atcatcaccc aggcgctgac cttcgcgttc atgctgctgt
     1921 tttttcgtaa cgacaccggt tttggcggca ataacggctt taccgacttc aaacggatct
     1981 tgggattttc tctggcccat ccaggcaccc gggtggcgct gtttgcgatt actggggcgg
     2041 tgttgatcct gactctgctg ctatgccgct acattgtcac atccaagttt ggccgcgtgc
     2101 tcacggcgat tcgcgacgcc gagtcgcgca cgatgttttc cggttacaac acccgcgact
     2161 acaagctttt tgtatggaca ttgtcggcgg cattgtgcgg catcgccggt gcgctctacg
     2221 tgccccaagt aggcatcatc aacccaagcg agatgtcgcc ggcgaactcg atcgagatcg
     2281 ccatctgggt ggccgtgggc ggtcgcggta ccctcgcggg tgcgatcctc ggcgccgcca
     2341 tcgtcaacgg cgccaagagt tatttcaccg tcgcttggcc ggaattctgg ttgtattttc
     2401 tcggcgcact gttcgtggtg gtgaccgtct atttgcctaa tggcgtgatg ggcttgacgc
     2461 gacggggcaa cccatgaggg cggagacaat gggtctggac ggtggtattg aaagcacggg
     2521 tatcagccac gtggtccagg ccgaagcgat cgatgtttcg cacaaggcga ttctttacct
     2581 cgagggagtg acggtcagct tcgatggttt tcgcgccctc aacgatttgt ctttttccat
     2641 cgatgtcggc gagctgcgtt gcgtcattgg acccaacggc gccggcaaga ccaccatgat
     2701 ggacgtcatc accggtaaaa cccgcccgga caccggcacg gtttttctcg gccaaaccat
     2761 cgatctcacc gccttgcccg agtacgaaat tgcccgcctc ggcgtcggcc gcaagtttca
     2821 aaaaccgtcg gtgtttgaaa atcatagcgt gtacgaaaat ctcgcgcttg caatcaaaag
     2881 cccaaagggc gtgtggaata cgctttttca ccggcgcgat tcggcgcaag aagatcgcat
     2941 cgacgcaatg ctccagcaaa ttcatctcaa agagcgcatg cgcgatcaag cggggctatt
     3001 gtcgcacggg caaaagcaat ggctggaaat cggcatgttg ttgatgcaag agccgcaagt
     3061 gctgctgctc gacgaacccg ccgcgggcat gagcgacgaa gagaccgagc gcacggcgga
     3121 attatttctg tccttggagg gaaagcattc tttaatcgtt gtcgagcat
//
LOCUS       DASQKS010000269         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_994674, whole genome shotgun sequence.
ACCESSION   DASQKS010000269 DASQKS010000000
VERSION     DASQKS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994674"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(461..1714)
                     /locus_tag="VE170_07000"
     CDS             complement(461..1714)
                     /locus_tag="VE170_07000"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15221.1"
                     /translation="MSEDHPRVQDPAPRLSPWASLRLRDYRLLFLLSLCATTAQQMRQ
                     AQNFYQVYEISGSAFQLGLTGVAQGVPIFVFGLFGGTLADFVDRKKLILATTLGNLLV
                     AVALGILTLAGLIEVWHIQVGTAMTSALNIVLNPTRMSLISYLVPRTHLTNAVSLNSA
                     ISQGSHFIGPMLGGLSLAWMSTGNAYLFNALFYLPAVFAIIALTIPTSIEVNREKLAL
                     ASLLGGVKFLFSEPLVLAMVLLDFVVIGVGYFRPLLPVFARDILFVGPAGFGMLSSAP
                     AIGGMIGTVTLLLFGDVKNKGMLALWSFLAYALAVGGFALSVNFWLSFGLLAVTGLAN
                     SLQAVMRQTTFHLLTPEHVRGRAFAVFHMFSQGANSVGATEVGFMAALVGAPGALLFG
                     GAIGTLLTVGCWIGMPNLRKFGAGD"
     gene            complement(1737..2897)
                     /gene="larC"
                     /locus_tag="VE170_07005"
     CDS             complement(1737..2897)
                     /gene="larC"
                     /locus_tag="VE170_07005"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HYJ15222.1"
                     /translation="MKIAYGDLIGGISGDMFVAALIDLGLPLAKLKAELKKIPTLEFA
                     LKTAKKSVHAVRASQFQVICPRHEAPRSWQQIRRLLERSKLAASVKATAIDIFNRLAQ
                     AEAKIHGVAVDQVHFHEVGATDSIVDIVAAAIGVGELAIDQLHFSPIPLGRGVTRSQH
                     GPLPVPGPATMELLKGLPTFGIDLDSETVTPTGAALLKVFGSSFGDQPAMTIDRIGYG
                     TGQKEFSSRPNLLRLSIGANAVNLQHEAMLVIETNIDDMNPQHFDHVMERLLAAGARD
                     VCLAPIQMKKNRPATLLTVICEIAQRDRLAAMILQETTTIGVRCYPVSRILLAREAKT
                     VKTRFGEVTVKIVTQPDGTERATPEYDDLKRIASAKRTTLKTVHDEVMRQLK"
     gene            complement(2980..4191)
                     /locus_tag="VE170_07010"
     CDS             complement(2980..4191)
                     /locus_tag="VE170_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYJ15223.1"
                     /translation="MRIETERKMKRWRDQRWILDQVIQTRGIDWDQGRTGKIIRNCGP
                     TVEKDLAELCSRIKKFVDIPREFSQAAAVREQQGRQAEAAGRLTEARDHYYIAACFYT
                     NAMWAVYEDGNRERIHWQERKRASYDKFIQFAGRPIERVELPYDGKKIHALLHLPPGR
                     QAGEKVPCVMYIPGMDGVKEDHPASGDPFIERGLALLSVDCPGHGETREGGIKCTASN
                     VEDVGRLVADYLISRPEIDADRLGIMASSMGSYWAPRVVAAEKRFKACAVSGVCMEPG
                     QFAIFNMSSPTFKLNYMYMSGYDDEAAFDEFCKTLSLEGVTAKITCPYMIVAGEDDEH
                     CDMKFVYKLMGEAPAPKLLYVFEGERHSIRNPKARLLVIDWLIDTLRGKPFKPEIIRI
                     ETSGKEVHRDW"
     gene            complement(4266..>4772)
                     /locus_tag="VE170_07015"
     CDS             complement(4266..>4772)
                     /locus_tag="VE170_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15224.1"
                     /translation="FGLNVKDVTMIALGGIPEILAAMRAGAVQAGILSPPTSTSARDL
                     GYRPLLHIPDLGREFTFSGIAAKRSFVHSQPEIVRAYMASLTDGAKVYKEDSRAALRI
                     LKKYLRADDRILEGGYKEYDAAISNPPYPGLKGLEAVRDSLLETTPQLKNVDLRKFID
                     DRFVKAAK"
BASE COUNT          979 a         1317 c         1490 g          986 t
ORIGIN      
        1 tgccagatcc ggctagggct gtgactatga aaatctcatg acgagtttgt tggatgggta
       61 attccgctac acccgtaagt gtccgaccat gtagatttaa tggagagcca atccatcgaa
      121 ggtttcgtct ccccgtccag aaaagaagat acagcgtgaa actctatatc gaagtcgtcg
      181 gttggttgtt ggtctgtcta atatttatcg tgggtatcgc caaagccttg ggacggctct
      241 aagcggccgc gtcaatcagc taccaatcaa ttttcacggc agtcatttta tgatttgacg
      301 ccaacgaatg agagtctctt gcggagccgc gccaagtgaa tcgaaactcc atcatgttgg
      361 gactgcgaat ctgcgcgagc gtttgacggg cacgactcaa agaataagtc ggcttccagt
      421 caccacgcaa ttcgacgaag tctggttgtt tcactcagct tcagtcgccg gcgccgaatt
      481 tgcgcaggtt cggcatgccg atccagcatc ccaccgtcag caaggtgccg atggcgccgc
      541 cgaagagtaa cgctccgggc gcgccgacga gcgccgccat gaagccgact tcggtggcgc
      601 cgacgctgtt ggcgccttgg gaaaacatat gaaacacggc gaaggcgcgg ccgcgcacgt
      661 gctccggcgt gagcaagtga aacgtggtct ggcgcatgac cgcttggagc gagttggcca
      721 agccggtgac ggcgagcagt ccgaacgaca accaaaaatt caccgatagc gcgaagccgc
      781 caaccgccag cgcgtaagcg agaaacgacc agagggcgag catccccttg tttttgacat
      841 cgccgaagag cagcaaggtg accgtgccga tcatgccgcc aatcgccggc gccgaggaca
      901 gcatgccgaa accagcggga ccgacgaaca gaatatctct cgcgaagacc ggcaggagcg
      961 ggcgaaaata gccgacgccg atgacgacga agtcgagcag gaccatggct aagaccagcg
     1021 gttccgagaa aagaaacttg acgccgccga gcaggctggc gagcgcgagt ttttcgcgat
     1081 tgacctcgat gctcgtggga atcgtcagcg cgatgatggc gaagacggcg ggcagataaa
     1141 aaagcgcgtt gaatagatag gcgttaccgg tgctcatcca agccaggctt aaaccgccga
     1201 gcatcggccc gataaagtgc gaaccttggg agatcgcgga gttgagcgac acggcgttgg
     1261 tcagatgggt tcgcggcacg aggtaggaga tcagcgacat gcgcgtcggg ttgagcacga
     1321 tgttgagcgc cgaggtcatc gccgtgccga cctggatatg ccaaacctcg attaagccgg
     1381 ccagggtcag gatgccgagt gcgaccgcga ccaacaaatt ccccaaggtg gtcgccagaa
     1441 taagtttctt acggtcgaca aaatcggcca gagtgccgcc gaacagaccg aagacaaaaa
     1501 tcggcacgcc ctgggcgacg ccggtgaggc cgagttgaaa cgccgatccg gaaatctcgt
     1561 agacttggta gaagttctgc gcctggcgca tctgctgcgc ggtggtcgcg cacagagaga
     1621 gcaggaaaag caggcggtag tcgcgcagac gcagcgaggc ccagggtgac agccgtggcg
     1681 ccgggtcctg cactcgaggg tgatcttcgg acatgacggg actcaagaag tcgtggctac
     1741 ttgagctgcc gcatcacttc gtcgtgaacg gttttaagcg tggtgcgttt ggcgctggcg
     1801 atgcgtttca ggtcgtcgta ttccggcgtg gcgcgctcgg tgccgtccgg ctgggtgacg
     1861 atttttaccg tgacttcgcc gaagcgggtc ttgaccgttt tcgcttcgcg cgccaagaga
     1921 atgcggctga cgggatagca gcgcacgccg atggtcgtgg tctcttgcaa aatcatcgcg
     1981 gccaggcgat cgcgctgggc gatctcacag atcactgtta gcaatgtcgc gggacggttc
     2041 tttttcatct gaatcggcgc caggcagaca tcccgggcgc cggcggccaa cagccgctcc
     2101 atgacatggt cgaaatgttg cgggttcatg tcgtcgatgt tggtttcgat gaccagcatc
     2161 gcttcatgct gcagattgac cgcgttggcg ccgatggaca ggcgcaacaa gttgggacgg
     2221 ctggaaaatt ctttttgtcc ggtgccgtag ccgatcctgt cgatggtcat ggccggctga
     2281 tcgccgaaac tgctgccgaa aacttttagc aacgccgcgc cggtgggcgt caccgtctcg
     2341 ctgtccagat cgatgccgaa ggtcggcagt cctttcaaca attccatggt cgccggcccg
     2401 gggaccggca gcgggccatg ctgcgaacgg gtcacgccgc ggcctagggg gatcggcgaa
     2461 aaatgcagtt ggtcgatggc taattctccg acaccgatcg ccgcggcgac aatgtcgacg
     2521 atggaatcgg tggcgccgac ttcatgaaaa tggacctgat cgacggcgac cccgtgaatc
     2581 ttagcttcgg cctgggcgag ccgattgaag atgtcgatgg cggtggcttt aaccgaagca
     2641 gctaatttac tgcgctcgag caagcggcgg atttgctgcc aggagcgcgg cgcctcgtgg
     2701 cgcgggcaga ttacttgaaa ctggctggcg cggacggcgt ggaccgactt cttcgccgtc
     2761 ttgagcgcga actccagggt tggaattttt ttcagctcgg ctttgagctt ggccagcggc
     2821 aagccgagat cgatcagcgc ggcgacaaac atgtcgccgc tgataccgcc gatgaggtcg
     2881 ccgtaggcta ttttcattgc aaatctcgcg cttacttcaa accgagtgga ggatggagaa
     2941 gcgaagatag aggttttcga tccgcgacca tctataaact taccaatcgc gatgcacttc
     3001 cttgccgctg gtttcgatcc ggatgatttc cggcttgaag ggtttgccgc gcaaagtgtc
     3061 gatgagccag tcgatcacca gcagccgcgc cttggggttg cggatcgagt ggcgttcgcc
     3121 ttcgaagacg taaagcagtt tgggcgcggg agcttcgccc attagtttgt aaacgaattt
     3181 catgtcgcag tgctcgtcgt cttcgccggc gacgatcata tagggacagg ttattttcgc
     3241 ggtgacgcct tcgagggaca aggttttgca gaattcgtcg aaggcggctt cgtcgtcgta
     3301 gccggacatg tacatgtaat ttaatttaaa cgtcggcgac gacatgttga agatggcgaa
     3361 ctggcccggt tccatgcaga ccccgctgac ggcgcaggct ttgaaacgct tttccgccgc
     3421 gaccacccgg ggcgcccagt aagaacccat gctcgaagcc atgatgccaa gacgatcggc
     3481 gtcgatttcc gggcgggaga tcagatagtc ggcgaccagt ctgccgacat cctcgacgtt
     3541 ggaggcggtg catttgatgc cgccttcgcg ggtttcccca tggccgggac agtcgacgga
     3601 taacagtgcc aagccgcgtt cgatgaacgg atcgccgctg gcagggtgat cctctttgac
     3661 gccgtccatg ccggggatgt acatgacgca tggcaccttc tcgccggctt ggcggcccgg
     3721 cggcagatgc aacagcgcat ggattttctt gccgtcgtag ggcagctcga cccgttcaat
     3781 cggccggccg gcaaattgaa tgaacttgtc gtagctggcg cgcttgcgct cctgccagtg
     3841 gatgcgctcg cggttgccgt cttcatagac cgcccacatg gcattggtgt agaagcaggc
     3901 ggcgatatag taatggtcgc gcgcttcagt tagccggccg gccgcctcgg cctggcgccc
     3961 ttgttgttcg cgaaccgccg ccgcctggga aaactcccgc gggatatcga cgaacttctt
     4021 gatgcggctg cacagctcag ccaaatcttt ctccacggtg gggccgcagt tccggataat
     4081 tttcccggtg cgcccctggt cccaatcgat cccacgggtt tggatcactt gatccaagat
     4141 ccagcgctgg tcgcgccagc gtttcatttt tctttcggtt tcaattctca taatacctcg
     4201 gagcgctcgc cctcggctcg cggttcaaag ttcaagggtt caaggttcaa ctcggagtca
     4261 tcgctctatt tggcagcttt gacgaagcgg tcgtcgatga attttcgcaa atcgacattt
     4321 ttcaattgcg gcgtagtttc taacaggctg tcgcgcaccg cctccagccc tttgagcccg
     4381 ggatagggcg gattggaaat cgccgcgtcg tattctttat agccgccttc gaggatgcgg
     4441 tcgtcggcgc gcaggtattt tttcaagatt ctcaaggcgg cgcggctgtc ttccttataa
     4501 accttggcgc cgtcggtgag cgacgccatg taggcgcgca cgatctcggg ctggctgtgc
     4561 acgaaggagc gtttggcggc gatgccggag aaagtaaatt cacggccgag atcggggata
     4621 tgcaacagcg gacggtagcc cagatcgcgt gccgaggtcg atgttggcgg tgacagaatg
     4681 ccggcctgga ccgcgccggc gcgcatggcg gcgaggattt cgggaatgcc gcccaaggcg
     4741 atcatggtga cgtctttgac gttcaaacca aa
//
LOCUS       DASQKS010000270         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3083522, whole genome shotgun sequence.
ACCESSION   DASQKS010000270 DASQKS010000000
VERSION     DASQKS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3083522"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /gene="gatB"
                     /locus_tag="VE170_07020"
     CDS             <1..1392
                     /gene="gatB"
                     /locus_tag="VE170_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYJ15225.1"
                     /translation="KIFCGCSTQFGREPNANTCPVCCGFPGVLPVLNKKVVEFAIKAG
                     LATHCQISRSSILARKNYFYPDLPKGYQISQYEMPICSDGHVDFELNGISKRVRLTRI
                     HMEEDAGKNIHDAHGDSSLVDLNRAGVPLLEIVSEPDLRTPEEAAAYLRSLRATLQYL
                     GVCDGNMEEGSLRCDANVSVRPAGTETLGTKIEIKNLNSFRAVERALQFEIERQSETI
                     NEGGTLVQETRLWDEHCEETRSMRSKESAHDYRYFPDPDLLPLVIPDSWIDEIRAELP
                     ELPAARKQRFFTEHGLPSYDVELLTSRKDVADFFDIAVKIHSNPKALSNWIVGDLFRV
                     LKERKLDNQLYIQKWPITAQQLAEMVRLIDQGKISGKIAKSVFEALLDGSKTPQQIVR
                     EKGLEQVSDFGSIVAAIDQVLAANTKQVEQYRAGNDKVFGFLVGQIMKASQGKANPQK
                     VNEILRDKLKAAG"
     gene            complement(1625..3733)
                     /locus_tag="VE170_07025"
     CDS             complement(1625..3733)
                     /locus_tag="VE170_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYJ15226.1"
                     /translation="MKINQVSQRLLLPMMLLVGACGVLDDSASKKPSRTGLIATPVTY
                     YSTSRARYLGSKYKDNVDRLVERIVRNSKTSALQFANNISSVGGIGFFTHSATKKPDE
                     RYLEVVLAVPETFEAKGAHSDKVSRLFTSYGSELLTILSGEIDIYQDNELTGYGLNLA
                     WRNIIAETAGNRVTLERAIVYLSKEKTRNFLRHEIDQNNLLADATIFAVEEDGPLNLV
                     SYRPPEVHQEFRPAIREDDISVASAANPAYALTMESPDHAKTDSLRVKEPPAKVAPPK
                     PPVILEQKPKTSPLRASVKLAPEAQSAAPTAMISAPVEASLVEAMTVEVARAKVEMLP
                     PIVAPSAGTKEVALVVKPIVKSDESARTAVDASKPLIAPQIVSPVPVEVASPTGVAIG
                     PKEKPSPLTTTAAAESKAIMPVVKPIVKSDKPVRDTNSSKPVVALQSTLQPPIERAPS
                     EVAAAAPKEVPARPAIQVKPIESGAPGRVFDSPKLLPVPLKPFLPPPVALYVPPPEAP
                     KVERSATPTVIAESIIPVEIASAAPVLTKPMPPQLSGETSWSAPAPQVEVISTLPVID
                     ASPRSVEAAPWPVVEKAPIKPAEQLALLTNKAPEAAQPKPAIARPATPKPLEGFIIQL
                     AFNDKERAQRWAEGMEKKGFAVSLTEAGAEGALRVRLGNFAIRDDAERQLKTFKQEGL
                     HGIILNLPQAFRPEARSSIP"
     gene            3883..5343
                     /locus_tag="VE170_07030"
     CDS             3883..5343
                     /locus_tag="VE170_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HYJ15227.1"
                     /translation="MALGELLRAKGIITQEQLDRVLHQQKIAGGRLGDNLVALGYLTR
                     GDLEAVISEPPPVPKTVDETGLESNFLLNCLLRTMYISALQTIPELAEQIMLTRGVID
                     ELLNFAKKEALVEIRGPSEKNYNIMRYALTNAGKERASEALRRCEYIGPVPVPLDVYQ
                     EQVQKQTITNEVIGIEKLKKAMSHLVLSPDIVRRLGPASNSGRAVLIYGAAGNGKTSI
                     AEALVSAFEQPVYIPYAIEEDGQIIKIYDPSVHVKFPTNNGVDSNGGFANPILMPHLE
                     YDPRWVRCKRPYLITGGELTLEMLDLEFDVHSKYYEAPLQMKAIGGVFVIDDFGRQRV
                     RPHELLNRWIFPLERKVDYLTLHTGKKITVLFDQLVIFATNFPPEELMDPAQLRRVHY
                     KMQIQPPTEKEFREIFQRICDSYGLEFNDEMMNYLLNSFYNKHKVPFAGFHPKFIAEH
                     VVAASNYLGTPPRITTELLTDSLQNMVVLPNSAVSE"
     gene            5350..6633
                     /locus_tag="VE170_07035"
     CDS             5350..6633
                     /locus_tag="VE170_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYJ15228.1"
                     /translation="MAKPLALIIQSSDMATTSISKVAESRDCSIHISRSIEEAAQALA
                     AYPSDQLKYVFIDLALCHGQSWNEFTEQLMQIPDRMALICYNPHHVQTLYGLFGQANK
                     SQSAEVNGPHSITAPHMIGDTPQFHEVVNLANRYAMHDITVLITGETGTGKEVMAQYL
                     HAHGPRADKPFVGCNMTAIPETLVESELFGYVRGAFTGADRNKKGFIEAAEGGTLFLD
                     EIGDLAPSIQLKLLRFLETRQYYRVGESSPKTSDVRIVAATNKELDEAIQGSGFRKDL
                     YFRLNSARIILAPLRERRQDIILLIANFIYQACNQFRKPLKKLTSSAKALFLDYPWPG
                     NIRELKSVIESAVMVSDGEYITISDLPMNLQHYATGHSEEIGSKAIRNIEEGERIVIE
                     EALRQTNGNKAKAAEALGISTRTLYRKLDKFSLPT"
     gene            6914..>7550
                     /locus_tag="VE170_07040"
     CDS             6914..>7550
                     /locus_tag="VE170_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15229.1"
                     /translation="MERKQQPMVSALLKHDSLDTSAIDLASTSAGGFGKMLNSLQGLQ
                     QRLEGFSITDVTNAEANAQTLTQRLRQFQDTITAVALLKSATVKVSRSIAEIAPYDTN
                     VVDLDSLENHPQLHAIVKASKLIKLQKLMRALKAGAEIGGSQEDGFHDEITTAVLPTV
                     ELTAIRQNDSLHGTVGELSKVDPTNSPWEVEESAEALQTLEIIPTDPFIIVE"
BASE COUNT         1859 a         2030 c         1938 g         1723 t
ORIGIN      
        1 aagatcttct gcggctgctc gacacagttc ggccgcgaac ccaacgctaa cacctgccca
       61 gtctgctgcg gctttccggg cgttctgccg gtactcaata agaaagtcgt cgagttcgcc
      121 atcaaagcag gtctcgcaac ccattgccaa attagccggt cgagcattct cgctcgcaaa
      181 aattattttt atccagactt gcccaagggc taccagatca gccaatacga gatgccgatt
      241 tgcagcgacg gtcatgtcga cttcgagtta aacggcataa gcaaaagggt gcgcttgaca
      301 cgtattcaca tggaggaaga cgcgggcaaa aatatccatg acgcccatgg cgactcgagt
      361 ttggtcgatt tgaatcgcgc cggcgtgccg ttgttggaaa tcgtcagcga acccgacctg
      421 cgcactcctg aagaagccgc cgcctatttg cggtcgctgc gagcgaccct ccagtatctc
      481 ggcgtgtgcg acggcaatat ggaggaaggc agtttgcgct gcgacgccaa tgtctcagtt
      541 cgccccgccg gcactgaaac tttaggtact aagattgaaa tcaaaaatct gaattcattc
      601 cgtgcggtcg aacgagcgct gcagtttgaa attgaacgcc aaagtgagac catcaacgaa
      661 ggcggcacgc tggttcaaga aacccgcttg tgggatgaac attgcgaaga aactcgctca
      721 atgcgctcga aagaatcggc gcacgactat cgctatttcc ccgatccaga tctgctgcca
      781 ttggttatcc ccgatagctg gatcgacgag atccgcgccg aattaccgga actaccagca
      841 gcgcgcaagc aacgtttttt caccgaacat ggtctgccgt cctacgacgt cgaactcctc
      901 actagccgca aggatgtcgc cgactttttc gatatcgcgg taaaaattca cagcaatcca
      961 aaagcactga gtaattggat cgtcggcgat ctatttcgag tgctgaaaga gcgcaagctc
     1021 gacaaccagc tctacatcca aaagtggccc atcactgcac agcaattagc cgaaatggta
     1081 cgactgatcg atcagggcaa gattagtggt aagatcgcca aaagcgtctt tgaggcgctg
     1141 ttagatggta gcaaaacgcc gcagcagatc gtccgcgaaa aaggtctaga acaagtgtcg
     1201 gactttggga gtatcgtagc cgccatcgat caagtgctcg ccgccaatac caagcaagtc
     1261 gaacaatacc gtgcggggaa cgacaaagtc ttcggcttcc tggtgggcca gatcatgaaa
     1321 gcgtcccagg gtaaagccaa cccgcaaaag gtaaatgaaa tcctgcgcga caagctaaaa
     1381 gcggcaggat aaaactaatt gccataagta tttttaagtc ctcgtgagcc atccggcgag
     1441 ttaacactgc cttgtgggtc aatggttaaa atccttagtt gctacgattt gtgcaccaag
     1501 catagggctg gctgcggtta ggattggcac caagggaaat aagctggcgc ggcgattgcg
     1561 cgttgtgtca acgccatcgg aaaccctcgg acaaggaatt caatttgaac tttacgctca
     1621 acagctatgg aatcgaagag cgtgcttccg gccgaaacgc ctgaggcaga ttcaagataa
     1681 ttccgtgcaa tccctcctgc ttgaaggttt tcagctgccg ctcagcgtcg tcgcgaatag
     1741 caaaattgcc aagccgaaca cgcagcgcgc cttcggcgcc tgcttcggtt agcgataccg
     1801 caaagccttt tttctccatc ccctcagccc aacgctgggc gcgctcctta tcgttgaacg
     1861 ctagttgaat aatgaatccc tcgagcggct tgggggtagc tggacgtgcg atcgctggtt
     1921 tgggttgcgc agcttctggc gccttattgg ttagtagtgc caattgttcc gcgggtttta
     1981 ttggagcttt ttcgacgact ggccacggtg ctgcctcgac tgaacgcggt gacgcgtcga
     2041 taacgggtag cgtgctgata acctcgactt gaggagcagg cgccgaccaa gaggtctcac
     2101 ctgacagctg aggcggcatc ggctttgtaa gcactggcgc cgcgctagcg atctcaacag
     2161 ggataataga ttccgcaatg acggttggcg tcgctgacct ttcaaccttc ggcgcttccg
     2221 gcggcggtac atataacgca acgggcggcg gcaagaaagg tttcaacggc actggcagca
     2281 gcttcggcga gtcaaaaact cgccctggcg ctccgctttc aattggcttc acttggattg
     2341 ccggccgcgc gggcacttct tttggcgccg cagcagcaac ctcgctcggc gcacgctcga
     2401 ttggcggttg caatgtactt tgcagcgcaa ctactggctt ggaggaattt gtgtcgcgca
     2461 caggcttatc actttttacg atgggtttga cgaccggcat aattgctttg ctctcggcag
     2521 cagcagtagt agttagcggc gatggctttt cttttggccc aatagcgaca ccagtaggcg
     2581 acgcgacttc aaccggcact ggcgatacga tttgcggagc aattagtggc ttggaggcgt
     2641 cgaccgctgt gcgcgccgat tcgtcacttt tcacaatcgg tttgacgacc agcgcgactt
     2701 ctttggtccc ggcagatggt gcaactatcg ggggtaacat ttccactttg gctctagcga
     2761 cttctactgt catcgcctcg acaagcgatg cttccacagg tgctgaaatc atggctgtgg
     2821 gcgcggctga ttgtgcctcc ggtgccagtt tgacggacgc tctaagcgga gaggttttag
     2881 gtttttgctc taggattacg ggcggctttg gcggcgcgac tttagccggc ggttctttta
     2941 cccggagcga gtccgttttc gcgtgatccg gcgattccat cgttaacgcg taagccggat
     3001 tggcagccga tgctacgctg atatcgtctt cacggattgc gggacgaaat tcctgatgga
     3061 cttccggcgg ccggtacgac actaaattaa ggggaccatc ttcttctacc gcgaaaatcg
     3121 tggcgtcggc caacaaattg ttctgatcaa tctcgtggcg caaaaaatta cgggtttttt
     3181 ctttagaaag ataaacaatc gctcgttcta atgtcacccg attccccgcc gtttcggcga
     3241 tgatattgcg ccatgccaga tttagtccgt agccggtgag ttcgttatct tgataaatat
     3301 cgatttcgcc gctcaaaatc gtcaacagct cagacccata tgacgtgaat agacggctaa
     3361 ccttatcgct atgagcacct ttagcctcga atgtttccgg cacagctagc accacttcga
     3421 gataacgctc atcgggcttc ttagttgccg aatgagtaaa gaagccgatc cctccgaccg
     3481 atgaaatatt attggcgaat tgcagtgccg atgtcttcga attgcgcacg atgcgctcga
     3541 ccaagcggtc gacgttgtcc ttgtacttgc tgccaagata acgcgctctt gaggtgctgt
     3601 aataagtcac cggcgttgcg attagcccgg tgcgggaagg ctttttgctc gcactgtcat
     3661 ccaacacccc acaggctccc acaagtagca tcatgggcag cagcaagcgt tgagaaactt
     3721 gattgatttt catccagcct ccgccgcaaa gtgcagcagc gtaaaaggtt tcttgacagg
     3781 ttttcgctag tacttataat gaaattttgt agattatact gaagaatata gttcttacag
     3841 atgctaaaga caacaaattt tttagccgac tgagagcaaa ttatggcatt aggtgaactg
     3901 ttacgggcaa agggaatcat cactcaagaa caacttgacc gcgtattaca ccaacaaaaa
     3961 atcgccggcg gtcggctggg cgataatcta gtcgccctgg gctatctgac tcgcggagat
     4021 ctcgaagcag tgatctctga accgccaccg gttccaaaaa ctgtcgatga gaccggcctg
     4081 gaaagcaatt tcttgcttaa ctgtctactg cgcacgatgt acatctcggc gctgcagacg
     4141 attcccgagc ttgccgagca gatcatgctg acccgcggcg tcatcgatga actgctcaac
     4201 ttcgccaaga aagaagcgct agtcgaaatt cgcgggccgt cggagaaaaa ctacaatatc
     4261 atgcgctatg ccttgaccaa tgccggcaag gagcgcgcca gcgaggctct acgccgctgc
     4321 gaatacatcg gccccgtgcc ggtaccattg gatgtttatc aagagcaagt acaaaagcag
     4381 accatcacca acgaagtgat cggcatcgaa aaattgaaaa aagcgatgtc gcatctcgtg
     4441 ctgtcgcccg acatagtccg ccgactgggg ccggcttcca actccggacg cgcggtcttg
     4501 atctacggcg ccgccggcaa cggtaagact tcgattgccg aagcgttggt gagcgccttc
     4561 gagcaaccgg tttatattcc ttatgccatc gaagaggacg gccaaatcat caagatttat
     4621 gacccttccg tccatgtaaa attccccacc aacaacggcg tcgacagcaa tggtggtttc
     4681 gctaatccta ttttgatgcc ccatctcgaa tacgatccac gctgggtgcg ctgcaagcgg
     4741 ccgtatctga tcaccggcgg cgagttgacg ctcgagatgc tcgacttgga atttgacgtg
     4801 cactccaagt actacgaagc gccactgcag atgaaagcca ttggcggtgt tttcgtcatc
     4861 gatgacttcg gccgacaacg ggtgcgccca catgagctat tgaaccgttg gatttttccg
     4921 ttggagcgca aggtcgacta tttgactttg cacaccggca aaaaaataac agtgctgttc
     4981 gaccagttgg tcatattcgc aaccaacttc ccgcccgaag aacttatgga cccagcgcag
     5041 ctgcggcggg ttcattacaa gatgcaaatt caaccgccaa cggagaagga gtttcgggaa
     5101 atttttcagc gtatttgcga ctcttacggc ttagaattca acgatgaaat gatgaactat
     5161 ctgttgaatt cgttttataa taagcacaaa gttccctttg ccggttttca tccgaaattt
     5221 atcgccgagc atgtggtcgc ggcgtccaac tacctgggca caccgccgcg tattaccact
     5281 gaattgttga ctgactcgtt acaaaacatg gtggtcttgc ccaattctgc tgtctcagag
     5341 taacccttga tggccaaacc gcttgccttg atcattcaat cgagcgatat ggcgaccact
     5401 agcatttcga aggtcgccga gtcgcgggat tgctcgatcc acattagccg ttcgattgaa
     5461 gaagcggcac aggcgctcgc agcctacccc agtgatcagt tgaagtatgt gtttatcgat
     5521 ttagcgttat gccacggaca gagctggaat gaatttaccg aacagctaat gcaaatcccg
     5581 gatcgcatgg cgttgatctg ctacaatccg caccatgtgc agacgcttta cggcttgttc
     5641 ggccaagcga acaagagcca atcagccgaa gtgaacggtc cccactctat cacagcgcca
     5701 cacatgatcg gcgacacgcc gcaatttcac gaagtggtca acctcgctaa ccgttacgcc
     5761 atgcacgaca tcacggtact gattaccggt gagaccggca ccggtaagga agtcatggcc
     5821 cagtacctcc atgcccatgg gccgcgcgcg gataaaccgt tcgtcggttg taatatgacg
     5881 gcgatccccg aaactctggt cgagagcgaa ctcttcggat atgtcagagg cgctttcacc
     5941 ggcgccgacc gcaacaaaaa aggcttcatc gaagctgccg aaggtggaac cttgttcctc
     6001 gacgaaatcg gcgatctggc gccgtcgatt cagctcaagc tgctccgatt tctggaaact
     6061 cgacagtact atcgcgtcgg cgaatcatcg ccgaaaacca gcgacgttcg catcgtcgcg
     6121 gcgacaaata aagaactcga cgaggcgatt cagggcagcg gttttcgcaa ggatctttac
     6181 ttccggctca acagcgcacg aatcatcttg gcgccgctgc gcgaacggcg ccaggacatc
     6241 attctcttga tcgcgaactt tatttaccag gcatgcaatc agttccgaaa gccgttaaag
     6301 aaactcacga gttcagctaa ggcgctgttc ctcgactatc cttggccggg taacatccgc
     6361 gaactcaaga gcgttatcga gagcgccgtg atggtctcgg atggcgaata cattaccatt
     6421 agtgatcttc cgatgaactt gcagcactac gccaccggcc atagtgaaga gattggcagt
     6481 aaagcgatcc gcaatatcga agaaggcgag cgcatcgtca ttgaagaggc gttgcgccaa
     6541 accaacggca acaaagcgaa ggccgctgaa gcccttggca tttccacacg cacgctttac
     6601 cgcaaactcg acaaattttc gctaccgacc tgagcccgga gacccctcag ggccgattga
     6661 cgatcatccc aaaaaacact tcaagcatat atccctaccg actgattttc ccaacaaaac
     6721 caaaacgata gctccctttt agcgacgtac aattttgttc gagcagaaca gaatgggtgg
     6781 agctatcgag taattgttgg acaattaagt ctttacctcg ctgaaaacgc tggtcttttc
     6841 tttcgggcat tcgtggcaac cgttttgcta cgttgagaac tagcgcgcca gttcgtacgc
     6901 catcgcgcgg tttatggagc gaaaacagca acccatggtc tcagcgctac taaaacacga
     6961 ttccctagat acgagcgcca ttgatttagc ttcaacatcg gccggtggat tcggcaagat
     7021 gctaaattct ttgcaggggc tgcaacaacg tcttgagggt ttttcaatta ccgatgtcac
     7081 taacgccgag gccaatgcgc aaacgttgac ccagcgcttg agacagttcc aggataccat
     7141 caccgccgtc gctttactga aaagcgcgac ggtaaaggtc agccgatcga ttgcagagat
     7201 cgccccatac gataccaatg tcgtcgatct cgacagcctg gaaaatcacc cacagctcca
     7261 cgccatcgtt aaggcgagta aactaattaa gctacaaaaa ttaatgcggg ccctgaaagc
     7321 tggcgcagag atcggtgggt cacaagagga tggatttcac gatgaaatta ccacggcggt
     7381 actgcccacc gttgagttga cagcgattcg ccaaaatgac tccttgcacg gcaccgtcgg
     7441 agagttgtcg aaagttgatc ctactaactc accatgggag gtcgaagaat ccgccgaagc
     7501 tttacaaacg ttagagatta ttccgacaga cccgttcata attgtcgaat
//
LOCUS       DASQKS010000271         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_138332, whole genome shotgun sequence.
ACCESSION   DASQKS010000271 DASQKS010000000
VERSION     DASQKS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138332"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VE170_07045"
     CDS             complement(<1..574)
                     /locus_tag="VE170_07045"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15230.1"
                     /translation="MICAAIMGATWPIIVAAAASRPLRITYLSNSVTMAPLWMAKETG
                     ALAKEGLDVEILSMTASVAVPALIANELDAVQMSAAPVLTAALRGHDLVFICGLLNTM
                     IWNFYGRPGIANAEALRGKIVGTDRPATPGGYATLVSLKKIGLGAKDVQLFPVGSSVY
                     VIAALHAGQVMAGIASPPASFQLEKMGYHQL"
     gene            complement(836..1885)
                     /locus_tag="VE170_07050"
     CDS             complement(836..1885)
                     /locus_tag="VE170_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15231.1"
                     /translation="MPRVPSIIFAAAVIAIRRQRRYVSIFGLILLLQLSVQSEVAAQS
                     IPLRYGQAISAAKSIFSLPTFVGEREGFFRRAGLNFKIIIPIPGGSDKMIDALHDDTV
                     DVTHVAVPFLIRAALNGSDAVAVAAEFTNPIYSLVVKPEIKNFADLKGKLIGLADEAG
                     TISISTRRLLALHGVNKDDFRVKIIEGTPTRWNCLKRGECDAVPLGQPQDLIAKKEGF
                     RVLGISNDAVPDFLYTVTATRRSWAEKNRDAVVRYIRALGATFKFIRDPANRKSVVKT
                     IVETTDTPADIAQQTLALFFEPERHVLPVQGEINVNGLGQVIAFMAEAGLLKPPLPSA
                     DRFVELKYLQAAGVK"
     gene            complement(1920..2330)
                     /locus_tag="VE170_07055"
     CDS             complement(1920..2330)
                     /locus_tag="VE170_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYJ15232.1"
                     /translation="MKSPAARANLGATLFAYLLLALSSAAWAGGEAGEDDHNHDDGPN
                     YFGFVKDTSGKTIRDAKVTADIKGRPSIVARTNAAGAYRIPGFGKDIAPANVAISCAK
                     EGYKQAKTLLKTRPSKTPPTAVEIECTMQRLGAK"
     gene            2793..3104
                     /locus_tag="VE170_07060"
     CDS             2793..3104
                     /locus_tag="VE170_07060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HYJ15233.1"
                     /translation="MIILHVTIQVKPEHIAEFLEVVRHDAEHSEMDEAGCLRFDVIQD
                     RDDANRFYLYEVYRDEAALAAHRETPHFKYYAEKTQPWLAAPPERRFGRNLIPADATW
                     R"
     gene            3186..4181
                     /locus_tag="VE170_07065"
     CDS             3186..4181
                     /locus_tag="VE170_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15234.1"
                     /translation="MKVSRHFSILASLFFVLAVCFGASAQEKVRLSHSALETSNAVWF
                     IAQELGFYKKHGLDSELLFIPSTTTSLTSLLAGDVHVANVSGGAVASAVLAGAQIAMV
                     ACYLNSLPYEMVVHESIKSAEELKGKSIGISRLGSASDVAARALLRGLNLEPDKQVPI
                     MQVGGSSERAAAFRAGRIAGFPSPPGVIHLTKGMPHRILISTADFKQRFEFPYICAAT
                     TKAYLQKNRETVKKVVSAHIEAVHFVKTRKDESKKIMAKYARTNNEDYLEAAYTASAK
                     LYDVVPLVTRPGVDVQIKEATSRKPGAQLRFEDMVDESIVKELDKSGFIDKVYKR"
     gene            complement(4279..5037)
                     /locus_tag="VE170_07070"
     CDS             complement(4279..5037)
                     /locus_tag="VE170_07070"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ15235.1"
                     /translation="MIPYRDENETQHPALITGAIVVLNIAAWLFVQGAGAELPLARSV
                     CNLGLVPGELTGLVPPGQAFPMGPGAVCLTDPGRQVSHLITSMFLHGGWMHLIGNMWF
                     LWLFGNNVEDSMGHLRFIAFYLLTGLAAALGQVVADPDSIVPMVGASGAISGVMGAYL
                     VLYPRVKVYTLVPIFIFFTSIALPAWAMLGYWFVIQLVSGLFSSGEMGGVAFWAHIGG
                     FVAGVVLVKLFARKDYVAAHRAHHWRPKRLMRDW"
BASE COUNT         1127 a         1391 c         1615 g         1084 t
ORIGIN      
        1 tcagctggtg atagcccatt ttctccaatt ggaaactcgc cggcgggctg gcgatgccgg
       61 ccatgacctg gccggcatgg agcgcggcga tcacataaac gctgctgccg acgggaaaca
      121 gctgcacatc tttggcgcca aggccgatct tctttaagga aaccaacgtt gcataaccgc
      181 caggcgttgc cggtctgtcg gtgccgacaa tcttgccgcg cagcgcctcg gcgttggcga
      241 tccccggccg gccgtaaaaa ttccaaatca tggtattgag taagccacaa atgaacacca
      301 gatcatggcc acgcagcgcc gcggtcagca ccggcgccgc ggacatttgc acggcatcga
      361 gttcgttggc aatcagcgcc ggtaccgcta cgctggcggt catcgataag atctcgacgt
      421 cgagcccttc tttagccagg gcgccggttt ccttggccat ccacagcggc gccatggtta
      481 ccgaattgct caagtaggtg atgcgcagtg ggcggcttgc cgccgctgcc acgattatcg
      541 gccaggtagc gcccatgatt gcggcgcaaa tcattagatg gataggccag cgctttattg
      601 gacgcaatag tcggacggtg cttcgagttg cgcgcagcga accgaagacg ccgcagtatt
      661 tgctgccatt cgggcgctgc tctttgtagc gcaatgattg ctttttggag attccccgga
      721 tgatcatggc gaccctgatt ggccgtaggt ctactacagc cgcttcccgt cgcgcaacca
      781 aaaatgttaa ctgcggccac gtgatccggc aacgcgcaaa gtccgggcgc cgaaattatt
      841 ttacgccagc ggcctgaaga tacttgagtt cgacgaaacg gtcggcactg ggcaagggcg
      901 gtttcaacaa gccggcttca gccatgaagg cgatcacttg gccgaggcca ttgacgttga
      961 tctcgccttg caccggcagg acatggcgct caggttcgaa aaacaaggcg agggtttgct
     1021 gggcgatgtc ggcaggggtg tcggtggttt cgacgatggt tttcacgacc gatttacggt
     1081 tggccggatc gcggatgaac ttgaacgtgg cgccgagggc gcgaatgtag cgtaccacgg
     1141 catcgcgatt tttctccgcc caggagcggc gcgttgccgt cacggtgtag agaaaatcag
     1201 gcacggcgtc gttggaaatt cccaggacgc ggaatccttc tttcttggcg atcaagtcct
     1261 ggggctggcc gagcggcacg gcgtcgcact cgccgcgttt gagacagttc cagcgcgtcg
     1321 gtgtgccctc gatgatttta acccgaaagt cgtctttgtt gacgccatgc agcgccagca
     1381 acctgcgggt ggagattgaa atcgtgccgg cttcgtcggc caggccgatt agcttaccct
     1441 tgaggtcggc gaagttttta atttccggct tgaccaccag actgtagatc ggattggtga
     1501 actctgcggc gacggcgacc gcgtcggagc cattgagcgc ggcgcgaatc aaaaatggca
     1561 cggcgacatg ggtgacatcg acggtgtcgt cgtgcagcgc atcgatcatt ttgtccgaac
     1621 cgccgggaat cggaatgatg atcttgaaat tcaaacctgc gcggcggaag aaaccttccc
     1681 gttcgccgac gaaggtcggc agggagaaga tgctcttagc cgcggaaatt gcctggccat
     1741 agcgcagtgg aatggattgc gcggcaactt cggattgaac actgagctgt agaagtaaga
     1801 tgagcccgaa tatcgaaacg taccgccgtt gacggcgaat cgcgattacc gccgccgcga
     1861 aaataatgga tggcacccga ggcatggcgg agtttcagcg cggtgtctga ctgtaatcgc
     1921 tatttggcgc cgagccgctg catggtgcat tcgatctcga cggcggtggg cggggttttg
     1981 ctcggccgcg tcttgagcag cgttttggct tgtttatagc cttctttggc gcaggagatg
     2041 gctacgttgg cgggcgcgat atctttgccg aaaccgggga tgcgataggc gccggcggca
     2101 ttggtgcggg cgacgatgga tggccggcct ttgatatcgg cggtgacttt ggcgtcgcgg
     2161 atggtcttgc ccgaggtatc cttgacgaag ccgaagtaat tcggcccgtc gtcgtggttg
     2221 tgatcgtcct cgccggcttc gccgccggcc cacgctgcgc tggaaagagc caaaagcaaa
     2281 tatgcgaaca gagtcgcgcc aaggttggcg cgggctgcag gtgatttcat aaatacccca
     2341 ttttcctctt ggcaatctcc aaatttttac cgcaagcaaa agctcatgag caggtaagcg
     2401 aaatttatca tgacagggga tgggatcaac cacgaccact gcggcgcgat gcgatcggtg
     2461 agagcgcgga cacggagcgc aagcgcgcgg cgcagaattg tgctccagtt cgtgccggca
     2521 gccagacggt tgtcgactga tgcgtcatgg ccccgagcga cgcgaccgcg agcaggccat
     2581 gattatgagc aatgcggcca tcatgggcaa ttggcttctg ttatcgattt aatccatgtc
     2641 gaagtggttg tccagtctgt atcaaacgcc gcaatactgg acaaggtcga gtggctgaga
     2701 tagcaagggg gcaagccgaa ggcagtcggc gcagaggtca gaaatgtccg ctatgcttta
     2761 ctcttagcac tcgattgtga cgggaggttt cgatgattat tttgcatgtg acgattcaag
     2821 taaagccgga gcatatcgct gagttcctgg aggtggtccg ccacgatgcc gagcattcgg
     2881 aaatggatga ggccggctgc ctgcgtttcg acgtgattca agaccgcgac gatgccaatc
     2941 gtttctattt atatgaagtt tatcgcgatg aggcggcgtt ggcggcgcac cgcgagacgc
     3001 cgcacttcaa atattacgcg gagaaaactc agccgtggct ggctgcgccg ccggagcgcc
     3061 gtttcggcag gaatttgatt cccgctgacg caacatggcg ttagcggttg tttttgcgcg
     3121 gcgcggaaga tcgtttaagt tggctttgtt acggcgcgta gactgagagt cctatgggag
     3181 gtcgtatgaa agtgtcgcgg catttctcta ttctcgccag tttatttttt gtcctggccg
     3241 tttgtttcgg cgcgtcggct caggaaaagg ttcgcctatc gcatagtgcc ttggaaacca
     3301 gcaatgcagt ttggtttatc gcgcaggagc tgggcttcta caaaaaacat ggcctggact
     3361 ctgaactgct tttcattccc agcacgacga ccagtttgac cagtctcctg gcgggcgacg
     3421 tgcatgttgc caacgtgtcg ggcggcgcgg tggcgagcgc ggtgttggcc ggggcgcaga
     3481 tcgccatggt cgcctgctat ctcaactccc tgccgtatga gatggtcgtc catgaatcga
     3541 tcaaatcggc ggaagaactc aaaggcaaaa gtatcggcat cagccgtctc ggcagcgctt
     3601 cggatgtggc ggcgcgggcg ctgttacgcg gtttgaactt ggaaccggac aagcaggtgc
     3661 cgatcatgca ggtcggcggc tcgtcggaac gggcggccgc ctttcgcgct gggcgcatcg
     3721 ccggatttcc atcgccgccg ggagtcattc atctgaccaa agggatgccg catcgaatat
     3781 tgatcagtac ggcggatttc aagcagcgct ttgagtttcc ttatatttgc gcggcgacga
     3841 cgaaagctta tttgcagaag aaccgagaga cagtcaagaa agtcgtatcg gcgcatatcg
     3901 aggcggtcca cttcgtcaaa acccgtaaag acgagagtaa aaagattatg gcgaagtatg
     3961 ctaggacgaa caatgaagat tacttggagg cggcttacac tgcgagcgcg aaactatacg
     4021 atgtcgtgcc cttggtgacc cggcccggtg tcgatgtcca gatcaaggaa gcgaccagcc
     4081 gcaagcccgg cgcacaactg cgcttcgaag acatggtcga cgaaagcatc gtgaaggaac
     4141 tggacaagag cgggtttatc gacaaagtct ataagagata acgagccacg acttctgcac
     4201 attcatggat tcggcccggt ttaaggcgca tcgacggtgc tcgtcgatgc gataattgcg
     4261 ggcgggccgc gcgagttttc accaatcccg catgagccgc ttcggccgcc agtgatgggc
     4321 gcggtgggcg gcgacgtaat ccttgcgggc gaatagtttg accagcacga cgccagcgac
     4381 gaaaccgcca atatgcgccc agaaggcgac gccgcccatt tcaccggacg aaaataaccc
     4441 gctcacgagt tggatgacaa accaatagcc gagcatggcc caggcgggca gggcgatcga
     4501 agtgaagaaa ataaaaattg gcaccagcgt atagactttg acgcgcggat agagcaccag
     4561 ataggcgccc atgactccgc tgatcgctcc ggaggcgccc accatgggaa cgatcgagtc
     4621 gggatcggca accacttggc caagtgccgc ggccaagcct gtgagcaggt aaaatgcgat
     4681 gaagcgcaga tgtcccatcg agtcctcgac attgttgccg aagagccaca ggaaccacat
     4741 gttgccgatc aggtgcatcc agccgccatg gagaaacatc gacgtgatca gatgagatac
     4801 ttgacggccg ggatcggtta gacagacggc gcccggcccc atcggaaagg cctggcccgg
     4861 tggcactaac ccggtcagtt cgccgggaac gaggccgagg ttgcacaccg atctggccaa
     4921 aggtaactcg gcgccggcgc cttgcacgaa cagccaagcg gcgatgttca agacgacaat
     4981 ggcgccggtg attaaggcgg ggtgctgggt ttcgttttca tcgcgatagg gaatcatggt
     5041 taccttctag cccaatttta aacaaaagcc gagggacttt cgtgaaatcc ggccccagca
     5101 gatatcgcgc aatcaatgtc gttttgacgc tgtgatcgtc ccccggacat caagtacgac
     5161 agtcaaagcc gaccggaagc attacattct tggcttgatt ggggtcggac cgcgcgc
//
LOCUS       DASQKS010000272         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6036306, whole genome shotgun sequence.
ACCESSION   DASQKS010000272 DASQKS010000000
VERSION     DASQKS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6036306"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1071
                     /locus_tag="VE170_07075"
     CDS             343..1071
                     /locus_tag="VE170_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ15236.1"
                     /translation="MRILVVEDDKKIASFIVKGLAEAGFAVDHADNGDEALHLALSES
                     YDAAVMDIMLPKLDGLSVIERLRQAKILTPVLILSAKRSVDDRVKGLQTGGDDYLTKP
                     FSFVELLARVQALIRRAGNETEPTRLAAGDLSLDLLTREVKRGDNRLDLQPREFALLE
                     YLMRNAGKVVSKTMILEHVWGYDFDPMTNVVDVLVSRLRSKLDRDFDPRNYLSLPAKD
                     SESRSRLKKTVSSATLGVSESAQP"
     gene            1396..1602
                     /locus_tag="VE170_07080"
     CDS             1396..1602
                     /locus_tag="VE170_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HYJ15237.1"
                     /translation="MQPGDTFLAWQFPLHNGEAFGLTGRIAVFVAGIIPALLYISGIF
                     IWWKKRKGRSRATRLRISRKLQST"
     gene            complement(1625..2101)
                     /gene="bfr"
                     /locus_tag="VE170_07085"
     CDS             complement(1625..2101)
                     /gene="bfr"
                     /locus_tag="VE170_07085"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HYJ15238.1"
                     /translation="MKGSPEVIKVLNEVLRKELTGINQYFIHSKMCKNWGYAVLAEHG
                     WNESIDEMKHADLLVERILFLEGVPNITAYDKILVGSTVKQQLENDLGLEQAALTVLK
                     PGIRTCLDVHDDATRELLEHIVTDEERHIDWIETQVHQIKEVGYENYLTQQIFKKS"
     gene            2549..3021
                     /locus_tag="VE170_07090"
                     /pseudo
     CDS             2549..3021
                     /locus_tag="VE170_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868477.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            3562..>4132
                     /locus_tag="VE170_07095"
     CDS             3562..>4132
                     /locus_tag="VE170_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYJ15239.1"
                     /translation="MSEKDVLDTKSATASASESENPAIPSPTPKRTRPRTNRDWWPDQ
                     LDLSILHAHSPVVDPMGKDFNYAEEFKKLDVNALKKDVEQVMTTSQDWWPADYGHYGP
                     LFIRMSWHAAGTYRIADGRGGGGDGAQRFAPLNSWPDNANLDKARRLLWPIKQKYGRK
                     ISWADLLVFTGNVAMESMGFKTFGFGFGRE"
BASE COUNT          949 a         1093 c         1174 g          916 t
ORIGIN      
        1 gcatcacccg aagtgatcag cgacgggttt gaaggattgg cacgggagct gcgcgacgtc
       61 gccgatcagc tcgacgcgat cgcgcggcgc atcaatcgcg tgctctaatt tttttacgaa
      121 atccccgccc tttttgtctg tgtcctcggg aagggctgtg ggataggggc atgagcgtcc
      181 ggcgcctagg ttggacgcca tgccccacta atttggactt cgaaaccaga tcggcggtca
      241 caccgccatt tacttaagct tcacggggga ctcggccgcc gtatcgatcg atatgcggcc
      301 ggcgagattg agttccatgc gactttgacg gtaactatat ccatgcgcat cctcgtcgtc
      361 gaagacgaca agaagattgc ttcattcatc gtcaagggct tggccgaggc cggcttcgcg
      421 gtcgaccatg ccgataatgg cgacgaggcg cttcacctgg cgttgtccga gtcctatgac
      481 gcagcggtca tggatatcat gctgcccaag cttgacggct tgagcgtgat cgagcggttg
      541 cgacaagcga aaatacttac gccggtgctg atcttgagcg ccaagcgcag tgtcgacgat
      601 cgagtcaaag gcttgcaaac cggcggtgac gattatctga ccaaaccatt ttccttcgtc
      661 gagctgttgg cgcgcgtgca ggcgcttata cgccgggccg gaaacgaaac cgagccgacc
      721 cggctggctg ccggcgacct gtctctcgac ttgctcaccc gcgaggtcaa gcgcggcgac
      781 aacagactcg atttacagcc gcgcgaattt gccttgctcg agtatctcat gcgcaatgct
      841 ggtaaagtag tgtcgaaaac gatgatcctc gagcatgtct ggggttacga tttcgacccg
      901 atgactaacg tcgtcgatgt gctggtatcg cgcttgcgca gcaaactcga tcgcgatttc
      961 gatcctcgga actatcttag tcttcccgcc aaggattcgg aaagtcgatc acgcctgaag
     1021 aaaactgtct cgtctgcaac actgggcgtc agtgagagtg cccaaccgta aaggcggaga
     1081 agttacggca tacgagcctt gctagcgtgt cacgtctctg agagatcggc gcacttcgcc
     1141 cggttgtcgt aaccagatgt tgtgaggtgc gtcatatttc agcgactcag aaccaaggct
     1201 acgcatcgga tcgcggtgcg tttcctgagc gattgcttgt cggcacatct caaggaacag
     1261 gcgaagctgt accttgaagg cctccgcata acattattga gataaaggcc taccaagatc
     1321 cttgtatgac cgcgggcggc cgtttggata gaccaatata acggtgaggt tttggcggcg
     1381 cgcgatgcgc tttaggtgca gccgggggat acgtttctcg cttggcagtt tccgctgcat
     1441 aatggcgaag ccttcggcct gactggccgc atcgccgttt tcgtcgcggg catcatcccg
     1501 gcgctgcttt atatcagcgg aatcttcatc tggtggaaaa agcgcaaagg acgatctcgg
     1561 gccacgcggc tcagaatcag ccgtaagctg caatcaacgt aacgccaaaa ccatgttctg
     1621 ctacttagct cttcttgaaa atttgctgcg ttagatagtt ctcgtagccg acttctttga
     1681 tctggtgaac ctgagtttcg atccagtcga tgtggcgctc ttcgtcggtc acgatatgtt
     1741 cgagtaattc ccgggtcgcg tcgtcgtgga cgtcgaggca ggttcttatt ccaggcttga
     1801 gaaccgtcaa cgccgcttgc tctaacccga gatcattctc caactgctgc ttgaccgtcg
     1861 atccgaccaa gatcttgtcg taagcggtga tgttgggaac gccttcgaga aatagaatcc
     1921 gttcaaccag caggtcggcg tgtttcatct cgtcgataga ctcattccag ccgtgctcgg
     1981 cgagcacggc gtaaccccaa ttcttgcaca ttttcgagtg aataaaatat tgattgatgc
     2041 cggtgagctc tttgcgcagc acttcgttaa gcaccttgat gacttcagga cttcccttca
     2101 tacgttacct ccataccgtt tatctaagca tggacgtcgg caaagaacaa agcggattaa
     2161 agacttttgt cgaatttttc caggcgccga tcgtcattcg gcattgcctg cgctagtgtt
     2221 agaaagacac atttataagc gccttggtca ttagtcggca aacgcacttg cgggcggcaa
     2281 gctgatgacg gcatcaacat tatcataatt gggttttttc aaagtgcaga ttttcaataa
     2341 tgacaattct attcttcggt cggcgtgaaa aatccgtgcg attgatcgtg gcacgtttac
     2401 tgctatcttt gtcatatcga gaggccagcg gtcacgaacc cagagcccgc ggattcgcac
     2461 gtctggcgaa tcgcgggctc tcgtcattta agggcgatga gttgccagcg cctaagagaa
     2521 tcagaaatga aacaaacagg agaacattgt gggtacacaa gaaagagcat tcgtcggcat
     2581 ggaggtggag caattattga agctgctcaa catggctttc gccgacgggt ggttggctta
     2641 ttatcaataa gtgaccggtg aaaaaagcgg tgatcgccga gttgctgcaa gacgccaccg
     2701 acgagttgcg ccatgaggat atggtcgctg agcgcatcat tcagctccgc ggcacgccgg
     2761 tgaccgaacc taagaaatgg cacgaagtcg gcagctgcgg cgacgctgct ccggatgatc
     2821 cgttcgtcga gacgatcctg aatcaaaaca gcaagggcga gcgctgcgcc atcggttttt
     2881 acaagaagct tttggaagcg acccagggga aagatcccgt gacctataaa attgtgcctg
     2941 cagattctcc aggatgaagt cgagcaccaa gaagatcttc aatccctcac ggaggatctc
     3001 gaattgatgc tgaagaagta gacgaagccg gactggtaaa atccgacgcg atcgcggcgc
     3061 gccgaatgaa aaggtgcaga gcgccgtcaa tttctggtag cccaaggggc tcgacatgtt
     3121 cggtcgctcg gtttcgggag cgatccgagc gctacgtcta ctggagaatc aaacgccgca
     3181 ccaacatcca ggcgcgggaa gagtacattc gagaggtgac tccgctgctc atgaagatcg
     3241 gcttgcagat gcctaatccc tgtgaagggg cggaaatctc tttaagatcc gccggaataa
     3301 tactgacgta acagcgacca agttgtgaac aacgtcgacg agaatgcggg tcacgatctc
     3361 acactttcag aacgcaacaa aagattcaaa tatatcgtat gagaggagga tcgtttatgt
     3421 tcatagaacg ttagcaatct agctacgcgt gagtgccgtg ccgctgggcc ttgatgcggt
     3481 cccggggtgc aaatcaacgg caacataggc ctcgctgtct gctattagtt gcagcgacca
     3541 gcaagaaagg ggaagggtga catgtctgaa aaggatgtac ttgataccaa gagcgcgact
     3601 gcgagcgcga gcgaaagcga gaatccagcg atcccctcgc cgactcccaa gcgcactcgg
     3661 cccaggacca accgtgactg gtggccggat cagctggatc tctcgattct ccacgcgcac
     3721 tctcccgtcg tcgatccgat gggcaaggat ttcaactacg cggaggagtt caagaaactt
     3781 gacgtgaatg ccctgaagaa ggacgtcgag caggtgatga cgacgtcgca ggactggtgg
     3841 ccggccgact acggccacta cgggccgctc ttcatccgga tgtcgtggca tgccgcaggc
     3901 acgtaccgca tcgccgatgg ccggggcggt ggcggcgacg gcgctcaacg cttcgctccc
     3961 ctcaacagtt ggcccgacaa cgcgaacctc gacaaggcgc gccggttact ctggccgatc
     4021 aagcagaagt acggccgcaa gatctcctgg gccgatctgt tggtcttcac cggcaacgtc
     4081 gccatggaat cgatggggtt caagacattc gggttcggat tcgggcgaga gg
//
LOCUS       DASQKS010000273         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1397246, whole genome shotgun sequence.
ACCESSION   DASQKS010000273 DASQKS010000000
VERSION     DASQKS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397246"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VE170_07100"
     CDS             <1..432
                     /locus_tag="VE170_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15240.1"
                     /translation="AEAIQAMWQRNLNVVVKIENQEWKVYLKKLQTDPFVVHRAGWGA
                     DYPDPDNFMKLFTANSGNNHGRWKNPKYDQLLDQAASESDSAKRTQLYDEAQKLLTET
                     DAAIAPLFWKAEATMLSPKFTGLEYNSMARMDLRNVKPSGK"
     gene            731..1222
                     /gene="accB"
                     /locus_tag="VE170_07105"
     CDS             731..1222
                     /gene="accB"
                     /locus_tag="VE170_07105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HYJ15241.1"
                     /translation="MELSEDDVLHILKLIDESKFDYFQLEVGELKITVSKGDPIPLGN
                     SAPPVAISAAPAASAPSVAPKPVPAPAASAPTPKAAAIPAGHVAVTAPILGTFYVAPE
                     PGAPPFVTVGQQVTEDTTCGLIEVMKVFNSVRAGVKGTVVEVIAPNGGFVEFGQPLII
                     VKP"
     gene            1300..>2221
                     /locus_tag="VE170_07110"
     CDS             1300..>2221
                     /locus_tag="VE170_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYJ15242.1"
                     /translation="MSVSRVLVANRGEIAVRVIRACQSLGIETVAAVSDADRESMAAK
                     MANRAVCIGPARSIDSYLRIENVIAAAQGTGCDALHPGYGFLSERAALAQACVDNKIT
                     FVGPSAGNITMMGDKLEARKVARNAGVPLVPGSDHARNPHEAAVLAKNIGYPLLLKAS
                     AGGGGRGIKLVLNEREIEDTFRMAAAEAGAAFGNDTLYMERYVGNARHIEVQILGDQH
                     GNVIHLGERDCSLQRRHQKIVEEAPAYAVPAAVREQICAAAATLARSIGYQSAGTIEF
                     IYDNDTRSYYFLEMNTRIQVEHPVTEMITGV"
BASE COUNT          519 a          638 c          619 g          445 t
ORIGIN      
        1 gccgaagcga ttcaagcgat gtggcaaagg aatttgaatg tggtggtgaa gatcgaaaac
       61 caggaatgga aagtctatct gaaaaaactt cagaccgatc ccttcgtcgt ccatcgcgcc
      121 ggctggggcg ccgactatcc cgatccggac aacttcatga agctgtttac agcaaacagc
      181 ggcaacaacc acggccgctg gaagaatccc aagtacgacc aactgctcga ccaagccgcc
      241 agcgaatccg acagcgcgaa acggacccag ctctacgacg aagcgcagaa gttattaacc
      301 gaaaccgatg ccgccatcgc gccgctgttc tggaaagctg aagcgaccat gctcagtccc
      361 aagttcaccg gtttggaata taactcgatg gcacggatgg atttgcgcaa cgtcaagcct
      421 tccggcaaat gagccttttt tgctttgagt tattgcataa tatcggctag ctgctaaccg
      481 gcttcgcccg aaccgcagaa tgtaaagttc gagttgaata gatcgatagt ggtatgtttc
      541 gacgagagtc actctctgtc gaggtgatca accggccgga tcagaggttt ccctcgttat
      601 tcgttcagtt gttttgatct aatctccctc tgagaatttg ccatccgcgg taaaataatg
      661 ttagttgcgg agaggcagcg taacgctgcc ctttcgatgc aaccaaacac ttgagagaaa
      721 ggtaaaattc atggaactca gcgaagatga tgtcttgcat atcttgaagc taatcgacga
      781 gtcgaagttc gattattttc agctcgaagt cggcgaattg aaaatcaccg tgagcaaagg
      841 cgatccgatt ccgttgggca actccgcgcc gccggtggcg atcagcgccg ctcccgccgc
      901 atccgcgccg tcggttgcac ccaaacccgt gcctgctccc gcggcttcgg cgccgacgcc
      961 aaaagccgca gcgatccccg caggacacgt cgccgtcacg gcaccgatcc taggaacttt
     1021 ttacgtggcg cccgaaccgg gcgcgccgcc atttgtgacc gtcggccagc aagtaaccga
     1081 agacaccacc tgtggtctga tagaagtcat gaaagtcttc aacagcgtgc gcgccggagt
     1141 gaagggcacc gtcgtcgaag taatcgcacc caacggcggc ttcgtcgagt tcggccagcc
     1201 attgatcatc gtcaaaccgt aactatttgt gaacctttgg gacagaccgc tttcaccgag
     1261 cgaaagcggt caatccctct tcttgcttcg ggagtttcca tgagcgttag ccgagtattg
     1321 gtcgccaacc gcggcgagat cgcggtacgg gtgattcgcg cctgtcagag tttagggatc
     1381 gaaacggtcg cggcagtatc ggacgcggac cgcgaaagca tggcggcgaa aatggccaat
     1441 cgcgctgtct gcatcggccc ggcgagatct atagatagct acctaagaat tgagaacgtc
     1501 atcgccgccg cccagggtac cggctgcgat gcgctccacc ccggctacgg ttttctttcc
     1561 gagcgggcgg cgttggctca agcatgcgtc gacaacaaga tcactttcgt cggtccgagc
     1621 gccggaaaca tcacgatgat gggcgacaag ctcgaagccc gtaaagtcgc ccgcaatgct
     1681 ggcgtaccgc tggtgcccgg ttcggatcat gcgcgcaatc cgcacgaagc cgctgtgctt
     1741 gctaaaaaca tcggctatcc cctactgctc aaagcctcgg ccggcggcgg cgggcgaggc
     1801 atcaaactag tcttgaacga gcgtgaaatc gaagacacct ttcgcatggc cgctgccgag
     1861 gcgggtgccg ctttcggcaa cgatacgctt tacatggaac gctatgtcgg caacgcgcgc
     1921 catatcgagg tgcagatcct cggcgaccag catggcaatg tgatccattt aggcgagcgc
     1981 gactgctcgc tgcagcggcg ccatcaaaag atcgtcgaag aagcgccggc ttacgccgtg
     2041 ccggcggcag tacgggagca aatatgtgcc gctgcggcga ccttggcgcg cagcatcggc
     2101 taccaaagcg ccggcactat cgagttcatc tatgacaacg acaccaggag ctattatttt
     2161 cttgaaatga acacgcgtat ccaggtcgag catccggtta ccgaaatgat caccggcgtc
     2221 g
//
LOCUS       DASQKS010000274         4983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4341127, whole genome shotgun sequence.
ACCESSION   DASQKS010000274 DASQKS010000000
VERSION     DASQKS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341127"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1095
                     /locus_tag="VE170_07115"
     CDS             367..1095
                     /locus_tag="VE170_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HYJ15243.1"
                     /translation="MNATILNFQLRSLRLVAATAVVILLVGPSNVLAGPLIGVGQNPA
                     PPAGPFLGNDSQNDTITVGNSTSGRISIVPDSNGPAWVKEFVNRDGQGWSTTGPQSMV
                     TVDEFILFSASNILNPFGDPRIYDWHEVIDPTFEDGANFKWAGGTFEFQGPSAPSFPG
                     TVSLDGKSIWFDFPAGAALFAPIRITKQLMWTGPAVTPGANGTNEYRIRIVEQPSVPE
                     PTSLMIACLAIVGTIGYARRRATT"
     gene            complement(1092..2642)
                     /locus_tag="VE170_07120"
     CDS             complement(1092..2642)
                     /locus_tag="VE170_07120"
                     /inference="COORDINATES: protein motif:HMM:NF039970.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HYJ15244.1"
                     /translation="MRHPWFFPTAAALFIVLVAGQLAIAQPAIHDKAAVEKASVNSAS
                     AMDPSTAKVPIKEVLIPASGGRNVPENNDYDNPESEFCHQRSKSTDNFILFWAKEYGE
                     DPMANEDARRRFDVNEILKESERFFDYYVNTLKWVDKEKSFATRYKFLIYIFGGAGGT
                     AYGGAIDNKIGAFWSPATRVNRGPYGVVAHELGHSFQAIGRADGAERFSGGSIGEMGA
                     QYMLWQVYPEWMTFENYHLRDFMQRTHLAFLHPDNQYHSCYPIEYWSNKHGVELFGRM
                     WREVKRGEDPVMTYKRLTSIRQEQFNDEMFDACRRFVTWDMPRIEKVAARYANQHTTM
                     LNTADDGWFQIAADKCPQNYGYNAIKLKVPATSTKVTLDFKGIVGAEGFKSYQPERAG
                     WRYGFLASLEDGSRVYSDVFSKPEAAAEFSVPANTKFLWLVVMGAPSEHWIHVSARGG
                     GRGRGRGRGGAAGEARGGQADATRGQADAKDAPAEAPPNTTEQWPYRFKLTGTAPDDS
                     IVQSTSAN"
     gene            3273..4115
                     /locus_tag="VE170_07125"
     CDS             3273..4115
                     /locus_tag="VE170_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15245.1"
                     /translation="MGGASSVSALGSIDIYSPPGTSVKLGSEITKNDILKGSVHPFRR
                     DRNTSVLAYFAPKYGFTVKEAADFLGYDHFNWLNIVTEHPFIGDFPYNTTLPLVDPPE
                     FFSDGTRYPVGPGVFADSKPFYFNERELGPTYSASAWQSWITPNGGDAFVFNDQPKDF
                     RLDRSIGQRISFATMLVGVRKDGKFDYLLPPFYWHTDFTGKVGGTEIQLFSTPVDYGE
                     LTGPGGVSEIAFYQGLSDMPPDLVQILTELGGEFAFVPEPSSAGFMFVFLFPLLRRTR
                     RTVS"
     gene            4188..4319
                     /locus_tag="VE170_07130"
     CDS             4188..4319
                     /locus_tag="VE170_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15246.1"
                     /translation="MTDEPLISLEEAAKLLGYKASGLRKIVTRTKRGGQGAKIKFFR"
BASE COUNT         1087 a         1392 c         1374 g         1130 t
ORIGIN      
        1 ggattgcaca ccaatcccgt atcacccggg accgaccgtg ccaaacaaac tcacccacca
       61 ctcgatgtaa tggtggggat cgaattcgtg ccgcagacga tgaacagggt agaaatacat
      121 cacgtcataa aagccgtccc aatgtgtcgc ttcgcgaagc caaaatgcgg tcgctatcgg
      181 gaccgctgct gagaatgtaa agcaagatag cgccgaggaa gaatagcaac cagcaggccg
      241 ttttccccat acacttaggt ttttcttgca tgagccaaag gctccgggtt aaactacggg
      301 tcatccgggt taattgcgct ataaagtgtt ttctgtttaa catccttcgg ccggagtctc
      361 gatccaatga atgcgacaat cttgaatttc caacttcgtt cactgcggtt agtggccgcg
      421 accgccgtcg tcattctttt ggttggtccc tcaaacgtgc tcgcggggcc gttgattggt
      481 gtggggcaaa atcccgcgcc gccggcggga ccctttctgg gcaacgacag ccaaaacgac
      541 acgatcacgg tcggcaattc cactagcggt cgaatttcca ttgttcccga ttcgaacggt
      601 ccggcgtggg tcaaggagtt tgtcaaccgc gacggccaag gctggtcgac gactggaccg
      661 caatcgatgg tgaccgtcga cgagttcatc ttgtttagcg ccagcaatat tctcaatccg
      721 tttggggatc cgcggattta tgattggcat gaagtcattg atccgacgtt cgaggacggc
      781 gcaaacttta agtgggccgg cggtacgttc gaatttcaag gtcccagtgc gcccagcttt
      841 ccaggcacgg tcagtctaga tggcaaatcg atctggttcg attttccggc cggcgcagcc
      901 cttttcgcgc ccattcgaat tacaaagcaa ctgatgtgga ctggcccggc agttacaccg
      961 ggcgcaaatg gcacgaacga gtaccgcatc aggatcgtcg agcagccgtc ggttcccgag
     1021 ccgacctcgc taatgattgc ctgtctggcg attgtcggca cgatcggata cgctcggcgc
     1081 cgcgctacta cctaattggc cgaagtggac tgaacgatcg aatcgtccgg cgccgtgcca
     1141 gtcaacttga atcgatacgg ccattgttca gtcgtgttcg gaggagcttc cgctggggca
     1201 tccttagcgt ccgcttgacc ccgggtagca tccgcttgac cgccgcgcgc ttcgccggcg
     1261 gcgccgccac gaccccgtcc ccgcccgcga ccgccgccgc gggcggaaac atggatccaa
     1321 tgttcgctcg gcgcgcccat gacgaccagc caaaggaact tggtgttcgc gggcacggag
     1381 aactcagccg ccgcctcagg ttttgaaaat acatcgctgt acacgcggct gccatcttcg
     1441 agggaagcca agaaaccgta acgccagccg gccctttccg gctggtagct cttaaaaccc
     1501 tcagccccaa cgatcccctt gaaatcgagc gtgacttttg tgctcgtggc cggcaccttc
     1561 aacttgatcg cgttgtagcc gtagttctga gggcacttat cggccgcgat ctgaaaccag
     1621 ccatcgtcgg ctgtgtttag catcgtggtg tgctgattgg cgtaacgggc cgccaccttt
     1681 tcgatacgcg gcatatccca agtgacgaac cggcggcagg cgtcgaacat ttcgtcgttg
     1741 aactgctcct gcctgattga cgtcaaccgc ttgtaggtca tcacagggtc ttcaccccgc
     1801 ttgacctcgc gccacatccg gccgaagagc tcaacgccgt gcttgttcga ccaatactca
     1861 atcgggtagc acgagtggta ctgattatct ggatgcaaaa acgccaggtg ggtgcgctgc
     1921 atgaaatccc gaaggtgata gttctcaaat gtcatccact ccgggtagac ctgccagagc
     1981 atgtactgag cgcccatttc gccgatcgag ccccctgaga accgctcagc gccgtcggcg
     2041 cggccgatgg cctgaaagga atgtcccagc tcatgggcga caacgccgta gggcccgcga
     2101 ttgacgcgcg tggccgggct ccaaaaggcg ccgattttgt tgtcgatcgc tccgccatac
     2161 gccgtgccgc ccgcaccgcc gaaaatgtaa attagaaact tgtaccgcgt tgcgaacgac
     2221 ttctctttat cgacccactt gagcgtgttg acgtagtagt cgaaaaacct ttcggactct
     2281 ttgaggatct cattgacgtc gaagcggcgg cgtgcgtctt cgttcgccat cgggtcctcg
     2341 ccgtattcct tagcccaaaa gaggatgaag ttatccgtcg atttactgcg ctgatggcag
     2401 aattcgctct cgggattatc atagtcgtta ttctcgggaa cgtttcgacc gccgctcgct
     2461 ggaatgagaa cttctttgat cggcaccttg gccgtggaag gatccatcgc actcgccgaa
     2521 ttgacagatg ccttttcaac ggcggccttg tcgtggatcg caggttgagc aattgcgagt
     2581 tgccccgcga cgaggacgat gaaaagagcg gcagcggtcg ggaagaacca aggatggcgc
     2641 atgctaggtt gtctccaatt ggggttcctg gagcttaatc cgcggcgcat ggggattcaa
     2701 tgcttccgag tgaattgccc ttccgagcga attgcgatgt gcaccgccga tcgaattttg
     2761 cacgatgtgc gggggtccca cattctcgtg caaaaatcgg cggattatgc acattctgtc
     2821 gcgagacttt ctcggcaatt gcagattcat ggccaatttt gaccgcgctt aattcaatga
     2881 atttaggttt attaccggtg gttttccgac gagcaaaaat gagccgggcc atcagctttc
     2941 accggccggt tctcagccgt ccgcgcgtcc aatccttgcg ctcgccctgg cctgaccaat
     3001 gattacgcta tcccacacag cgtgcgcact ttgccggatg cttgacgggt caatttcatc
     3061 atgccggcga tactataccg gcgtatatag agcttaacag atcaggtggc caaaagcgag
     3121 atttggtcgc acgccgatca accgggacga ccgtcgccga cttcgcctgg cttaccgatt
     3181 tgcaggatcg gcgaaaaggc aaatgtcgtg gtaaacaacg cacagaataa atatgcttcg
     3241 gctcactatc tccggcaggc gttcggaaca cgttgggcgg cgcgagttcc gtatcagcct
     3301 tgggaagtat tgacatttac tcacctccgg gcacatcagt caagctgggg tctgagatca
     3361 ctaagaatga cattctcaaa gggtcggtgc atccgtttcg cagagatcgt aatacctcgg
     3421 tgcttgccta cttcgcgccg aaatatggtt ttacagttaa ggaagctgcg gactttctcg
     3481 gctatgatca ctttaattgg ctgaatatcg ttacggagca tccgttcatt ggtgacttcc
     3541 catataacac cactttacct ttagtcgacc cgccagagtt cttcagcgat ggaactcggt
     3601 atcctgttgg tcccggcgtg tttgccgatt ctaagccgtt ttactttaac gagcgggaat
     3661 tgggaccgac atattcggcc tctgcatggc agtcatggat tactccgaat ggcggcgatg
     3721 ctttcgtatt caacgaccag ccgaaggact ttcgacttga ccggtcgatt ggacagcgta
     3781 tatcgtttgc cacgatgctc gtcggcgtgc gtaaggacgg caaattcgat tatttgctgc
     3841 ccccatttta ttggcacact gacttcaccg ggaaagtcgg aggaacggag attcagttgt
     3901 tctcgacacc tgtcgattac ggtgagctta ccggtccggg cggagtatcg gaaatagcgt
     3961 tctatcaagg tctctcagat atgcctcccg accttgtcca aatccttacc gagcttggtg
     4021 gagaatttgc cttcgtaccg gaaccctcgt cagccgggtt catgtttgtc ttcctctttc
     4081 cgctgctaag gcgcacacgc cgcactgtct cttagtgcaa gccgacaacg cattgcaacg
     4141 agtgatgcgc aaggtctgtc gcaacacggt cggattcggg tcatagcatg acagacgaac
     4201 cgctcatatc gcttgaagaa gctgcgaagt tgcttggcta caaggcgagc ggcctgcgca
     4261 agatcgtcac gcgaacgaag cgtggtggcc aaggcgcaaa aatcaagttt ttcaggtagg
     4321 gcaggggccg atcaagttta gacgcgaatg gatagaggaa ttcatccaag cgaataccca
     4381 tgagccgaag ccaattaacc gctcgccggc tcaaaagcgc aaaccgacgc caaaggtgga
     4441 atcgcgattc ggactcagcc cttccatgta ctggtcaaat cagccgtggg ctaagggcaa
     4501 gtcgagcctg agcgattgcc gcgacgacgc caaatcgcca aaagactaat gcgtttctcg
     4561 ccgtggggcg gccgagccga cggcggaggt tcgttgccct tttctaacgg gcttctgcgc
     4621 gctaccttga tatcacgtgc gaagggattt gcgccagcgc cgtgagcgga ggtagaatgg
     4681 agaagtaatg gcggggacgc ggtcgcaagc gcggtcaaat tgttctacgc tcgcgacaaa
     4741 ccgctgccca tgacccaatg cggccgacac cgacgaaaag tattgaacgt cagcggagct
     4801 tgatgacttt gacatattta tcgacactgc accgacaact tatcaaccga caaccacggc
     4861 ttaatttacc atcgcttgcg gctttcccgc gaattgtggt attatcgcgt gaatggagag
     4921 caacggccag cggcagggaa tcctaatcgc atttgaaggc attgacgggg ctggcaagac
     4981 cac
//
LOCUS       DASQKS010000275         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3922783, whole genome shotgun sequence.
ACCESSION   DASQKS010000275 DASQKS010000000
VERSION     DASQKS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922783"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VE170_07135"
     CDS             complement(<1..879)
                     /locus_tag="VE170_07135"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15247.1"
                     /translation="MAGEYFSDFRDYLKALESRGKLHTWTRPVNKDSELMPLMRLQYR
                     GIADDRRQAFLYENVFDSRGKKHDLRVATGVYGSSREIAALGLGCDQPLEIYEKWRNA
                     LAKPIEPRRIASAPVHEIVYSGEGLKKFGVTGLPAPVEEPGFSGGIRATAPFITVDPQ
                     SGVRNVGMYSGHFRSDHHLIAGIAPVHHAMLYHHRSAASRKEPLPVAIVLGSLPDVTF
                     VSAANLPYGVDELAVAGGIRGRPVDVVQCKTIPLEVPAEAEIIIEGEISIERKERGEP
                     FSDYPGYLMVEREFRSV"
     gene            complement(915..2057)
                     /locus_tag="VE170_07140"
     CDS             complement(915..2057)
                     /locus_tag="VE170_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15248.1"
                     /translation="MNKQILISVPRWVAFQFLVLAWLCASAPQLFSQATKPFAPADWE
                     RIVDAGRKEGKVTVSIPASAELRKQLEEHFRKRFGIEVEVFTARGSAGVRRMADEFKS
                     GVRYFDLHIGGSSSAVSGMLDEGILDAIEPWLILPEVKEPKQWWGGHMWVDNGQKFIY
                     MFQAYLTESIWINTDLAKPTDFRSYDDFLQPKWKGKIGFLDPRTPGAGDSNWAHMWAV
                     KGEDYLKKFIAQEIVLGRDQRLLGENLAKGNVAVMIGLTSYSLHPFIKAGLPVKPVQA
                     LKEGTYGTGGSGNLAIIKAPAHPNATKVFVNWLLSRDGQEIVAKALGQATRRLDVDTK
                     WLRETGVIAAKDQMTVKEFLQVENQSEEKLDKVREPAQKLAAALLK"
     gene            complement(2340..4196)
                     /locus_tag="VE170_07145"
     CDS             complement(2340..4196)
                     /locus_tag="VE170_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ15249.1"
                     /translation="MKTTKKALKASSGQANTAAIDRPENLGKPQMKWGSDVVAETTRR
                     LNLKYIALVPGASYRGFHDSIVNYLGNENPQMVICLHEEHAVSIADGYGKVTETPMAV
                     ALHANVGLLHASMPIFNAWCDRTPMIIFGATGPVDAHLRRPWIDWIHTAKDQGAVIRD
                     YTKWDDQPASAQAAVESVLRANQITRTAPRGPVYICLDAGLQESALTDAIVIPDASRF
                     APAPTPAVAPASIEQTLRAIEQAQFPLILAGRVSRDQGDWDRRVRFAEAIGAAVLTSS
                     NDPSAFPTTHPLHFAAPCVRPNKTATALIEKADLIISLDWLDLAGVLRLSLGKSQTQE
                     PAAKTIVNCSVDSYRTNGWSMDHQALAAVDIPILAEPDQFVAQMLDALGGKKAGKMKP
                     RAAMKGLSHWNANKFAKPSSKEQMTMWDMAMTIRDYAQGKDVTFARLPIGWPGEALEF
                     DGPLSFMGNDGGGAVGTGPGHTIGAALALKDSGRLTIGVIGDGDYLMGVNALWTAAHF
                     NIPVMIVAADNRSYFNDELHQERVANMRSRPPQNRWIGQQIDHPHVDLVAMARAQGFD
                     SEAPVTTADGLMKALKNGAAIVAKGGRYFIDSLVQPGYADSGPDQRASLGKK"
     gene            complement(4286..4657)
                     /locus_tag="VE170_07150"
     CDS             complement(4286..4657)
                     /locus_tag="VE170_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15250.1"
                     /translation="MGLGIACKKTNWKGNNLMSKLTITGLAIAALMLPGLVAAQSTPR
                     VDQRQESQEKRIDKGVASGKITQKEAARLEKGQDRVQKTEDRAMKDGKLTKQEKRRIE
                     HTQDVQSKNITRESRDKQTAK"
BASE COUNT          957 a         1439 c         1339 g         1089 t
ORIGIN      
        1 caccgagcga aactcccgct cgaccatcaa gtagccggga taatcgctaa acggctcgcc
       61 gcgctccttg cgctcgatgg aaatttcgcc ttcgatgatg atctcagcct cggccggaac
      121 ctccaacgga atcgtcttac actgcacgac gtcaaccggt cggccgcgga tgccgccggc
      181 gacggcgagt tcatcgacgc cgtagggcag gtttgccgct gagacaaacg tcacatcggg
      241 caacgagcca agcacgatcg ccaccggcaa tggctccttg cggctggccg cgctgcgatg
      301 gtggtagagc atcgcatgat gcaccggcgc gatgccggcg atcaaatgat gatcgctgcg
      361 aaagtggccg ctgtacatgc cgacattgcg cacgcccgac tgcggatcga cggtaataaa
      421 cggcgcggtg gcgcgaatgc cgccgctgaa tcccggctct tccaccggcg caggcaagcc
      481 ggtgacgccg aattttttca acccttcgcc gctataaacg atctcatgaa ccggcgcgct
      541 ggcgattcgg cgcggctcga tcggtttcgc cagagcgttg cgccattttt cgtagatttc
      601 taacggctgg tcgcagccga gaccgagggc agcgatctcg cgcgacgagc cgtagactcc
      661 ggtggcgacg cgcagatcat gcttcttgcc gcggctgtcg aaaacgtttt catagagaaa
      721 agcttggcgc cggtcgtcgg cgatgccgcg gtactgcagc cgcatcaacg gcatcaactc
      781 gctgtccttg ttcactggac gagtccaggt atggagtttg ccgcgcgatt ccagcgcctt
      841 aagataatcg cggaaatccg aaaaatactc tcctgccatg ccccacactc cccttagccg
      901 ccgccggttt gaaattattt tagtaacgcc gccgccaact tctgcgccgg ctcgcgcacc
      961 ttgtcgagtt tttcttccga ttggttctcg acctggagaa actctttgac agtcatctga
     1021 tccttggccg cgatcacacc ggtttcgcgc agccacttgg tatcgacatc caagcgccgg
     1081 gtagcttggc ccaacgcttt ggcgacgatc tcctggccgt cgcgggacaa cagccaattg
     1141 acgaaaactt tcgttgcatt gggatgggcc ggcgctttga tgatcgccag attgccgctg
     1201 ccgccggtac cgtaagtgcc ttccttaagt gcttgcacag gtttgacggg caatccggct
     1261 ttgatgaacg gatgcaagct ataagaggtc agaccgatca tcaccgccac gttgccttta
     1321 gcgagatttt cgccgagcag ccgttgatcg cggccaagaa cgatctcttg ggcgatgaac
     1381 ttttttaaat aatcttcgcc cttcaccgcc cacatgtgag cccaattgga atcccccgcg
     1441 cccggggttc gtggatcgag aaagccgatc ttgcctttcc atttcggctg caagaaatca
     1501 tcgtaggaac ggaagtccgt cggtttcgcc agatcggtgt taatccagat gctctcggtg
     1561 agataggctt ggaacatgta gataaatttt tgcccgttat cgacccacat atggccgccc
     1621 caccactgct tgggttcctt cacttcgggc aaaatcagcc aaggctcgat ggcgtcgaga
     1681 atcccttcat cgagcattcc cgacaccgcc gatgacgacc cgccgatatg caagtcgaag
     1741 taacgcacgc cggacttgaa ctcatcagcc atgcgccgca cgccggcact cccgcgcgcc
     1801 gtaaacactt cgacctcgat gccgaagcgc ttgcgaaaat gctcttccaa ctgcttgcgc
     1861 agctccgcgc tggccgggat cgagaccgtg accttacctt ctttgcgtcc ggcatcgacg
     1921 attcgttccc aatccgcagg ggcaaacggc ttggttgctt ggctaaacag ctgcggtgca
     1981 ctggcgcaga gccatgcgag cactaagaac tgaaacgcga cccatcgcgg cacactaatc
     2041 aggatttgct tgttcactaa aatacccacg accaattggc tggaataata aatctttaga
     2101 agtaaatatt atatatgtca agtaataatc acctgtttta tcaagcacta gcgatgcacc
     2161 gaggaactca gctgcttatt cggtcaaacg gcccggggaa gtgcgagggg agatttaggc
     2221 gcaccgataa tcgctcaacg atgaaccgct gaaatgccgt cagcatgggt tatcgatggt
     2281 ctcgagccca tgctgacgtg agtcagaaga tggctttggt tgctgaaagg attgacgagc
     2341 tatttctttc ccaagctcgc ccgttgatcg gggccgctgt ccgcgtaacc cggttgcacc
     2401 agcgaatcga taaagtatcg gccgcccttg gcaacgatgg cggcgccgtt tttgagagct
     2461 ttcattaggc cgtcggcggt ggtcaccggc gcttcactgt cgaacccttg ggcccgcgcc
     2521 atggcaacca gatccacgtg cgggtgatcg atttgctggc cgatccaacg gttctgaggc
     2581 ggacggctac gcatgtttgc gactcgctcc tgatgcaact cgtcgttgaa ataagagcgg
     2641 ttatccgccg ccacgatcat caccggtatg ttgaaatgag cggcggtcca caaagcgttg
     2701 acacccatga gataatcgcc atcgccgatg acaccgatgg tgagccggcc ggagtcttta
     2761 agcgccagcg cggcgccgat ggtatggccc ggcccggtgc cgacggcgcc gccgccgtca
     2821 ttacccataa acgataacgg tccgtcgaac tccaaagctt cgcccggcca gccgatcggc
     2881 agacgggcaa aggtcacatc tttcccttgg gcgtagtcac gaatggtcat ggccatgtcc
     2941 cacatggtca tctgttcttt gctcgaaggt ttggcgaatt tgttagcgtt ccagtggctg
     3001 agccctttca ttgccgcccg cggcttcatt ttgcccgcct tcttgccacc cagggcatca
     3061 agcatctgcg caacgaattg atcgggttcg gcgaggattg gaatatccac cgcggcaagg
     3121 gcctgatgat ccatgctcca accattagtc cggtagctgt cgacggagca gttgacaatg
     3181 gttttggccg ccggctcctg agtttgcgat ttgcccaagc tcaaacgcag tacgccggcg
     3241 agatcgagcc aatccaggct gataatcaaa tcagcctttt caatcaacgc cgtcgccgtt
     3301 ttgttgggcc gcacgcaagg cgcagcgaag tgcagcggat gggtggtggg aaatgccgac
     3361 gggtcgttgc tgctggtcaa taccgcggcg ccgatcgctt cggcgaagcg cacgcgccga
     3421 tcccaatcac cctgatcgcg cgacacacgt ccggccagga tcaatgggaa ctgcgcttgc
     3481 tcaatggccc tcaaggtttg ctcgatcgat gccggtgcta ccgccggtgt cggcgccggc
     3541 gcgaaacgtg aagcgtcggg aatgacgatg gcgtcggtaa gcgcggactc ctgcaaaccg
     3601 gcatcgagac agatatacac gggcccgcgc ggcgccgtgc gggtgatttg attggcgcgc
     3661 aacaccgact ccaccgccgc ctgggcggac gccggctggt cgtcccactt ggtatagtcg
     3721 cgaatcacag cgccttgatc cttggcggtg tgaatccagt cgatccatgg gcggcgcaaa
     3781 tgcgcatcca ccggcccggt ggcgccgaat atgatcatcg gcgtgcgatc gcaccaggcg
     3841 ttgaagatcg gcatggacgc gtgcagaaga ccgacattag cgtgcaacgc cactgccatc
     3901 ggcgtctcgg tcaccttgcc gtacccgtcg gcgatggaca ccgcatgctc ttcatgcagg
     3961 cagatcacca tctgaggatt ttcattgccg agataattga cgatgctgtc atggaaaccg
     4021 cgataactcg cccccggcac caaagcgata tatttgagat tgaggcgccg ggtcgtctcg
     4081 gcgaccacgt cgctgcccca cttcatctgc ggcttgccga gattctccgg cctgtcgatg
     4141 gctgcggtat ttgcttgacc gctgctcgcc ttgagcgcct tcttggtagt tttcatctat
     4201 cgctccgcgc cgaattgatt ctcggcttat gacgtcctgt tgataagctt ctcggggcac
     4261 tcgaagtgaa gctttaatcg ggcttctact tagcggtttg tttgtcacgc gactcgcgcg
     4321 tgatattttt actctgaaca tcttgagtat gctcgatacg gcgtttttcc tgcttggtta
     4381 atttgccgtc tttcatggct cgatcctcgg tcttttgcac gcggtcctgc cccttctcaa
     4441 ggcgtgcggc ttccttttgg gtgatcttgc cggatgcgac gcccttgtcg atgcgttttt
     4501 cctgactttc ctggcgctga tcaacgcgcg gcgtggattg tgccgcaacc aaaccgggta
     4561 gcatcagcgc cgcaatcgcc aatccagtga tagttaattt actcatgagg ttattccctt
     4621 tccaatttgt ctttttacac gctatgccga gccccatttt ggttcatgcc aattgcgcgt
     4681 gtatatatga aggtcgcgta gttgtccaat ccgattgacc gctgcgaggg ggtttgccca
     4741 gccgctgaca atcaccgccc agcttgcgac ccaacttttt tccgagcccc tgcgagataa
     4801 ccgttttcct caagcgcgtt gtca
//
LOCUS       DASQKS010000276         5923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5177024, whole genome shotgun sequence.
ACCESSION   DASQKS010000276 DASQKS010000000
VERSION     DASQKS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5177024"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VE170_07155"
     CDS             <1..687
                     /locus_tag="VE170_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HYJ15251.1"
                     /translation="NGELGPLVALVILVLSVAVIGAIIFVERGQRRIPVQYAKRVVGR
                     RMYGGMTSHLPLKVNTANVIPIIFASSVLIFPATMAQFAAHPWIQTMSGMLAPGQWLY
                     EIAQIALIIFFAFFYTAVVFNPSDVAENMKKAGGYIPGIRPGQKTAEFIEKVLSRITL
                     SGAVYLCLIALLPSLLVRQFNVPFYFGGTALLIVVGVALDTVAQMETHLISRNYEGFM
                     KRGRLRGRKG"
     gene            690..1355
                     /locus_tag="VE170_07160"
     CDS             690..1355
                     /locus_tag="VE170_07160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HYJ15252.1"
                     /translation="MAAKARVVLLGPPGAGKGTQAKLLEQEFGAVQISTGDILRKAVA
                     DQSPLGKQAEDYIKRGALVPDDLITNLIAERLQQADCAQGFLLDGFPRTIPQAESLDA
                     LLMKLALSLNGVLSVQVPRAVIIERLAGRRTCKNCGALCHTVFDPPKQDGVCDRCGGE
                     LFQREDDKEETIANRLTVYDGQTAPLVDYYRRQGILREIDGVGSIEEIRSRIKKALGG
                     IAT"
     gene            1352..2110
                     /gene="map"
                     /locus_tag="VE170_07165"
     CDS             1352..2110
                     /gene="map"
                     /locus_tag="VE170_07165"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYJ15253.1"
                     /translation="MISLKSAREIEIMRRANVIVAEVLQELKRRVAPGITTLDLDAIA
                     EEMTLNRNAIPAFKGYNVAGRVYPRCLCASVNDEIVHGIPSNRHLREGDIVGLDYGVI
                     YDGFYGDSAMTVAVGKVSAAAQTLLDVTEASLYAGIEQLHEGKRLGDLGAAVQKVAED
                     AGYSVVRAFVGHGIGKKLHEEPPVPNYGEKDRGLRLKEGMVLAVEPMVNIGGYEVEIK
                     EDGWTAVTKDGSLAAHFEHSIAITKNGPYILSQL"
     gene            2148..2366
                     /gene="infA"
                     /locus_tag="VE170_07170"
     CDS             2148..2366
                     /gene="infA"
                     /locus_tag="VE170_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HYJ15254.1"
                     /translation="MAKQDAIEVTGTVLETLPNAMFRVELDNHHKVLAHISGKMRMHY
                     IKILPGDKVKIELSPYDLARGRIVFRER"
     gene            2381..2494
                     /gene="rpmJ"
                     /locus_tag="VE170_07175"
     CDS             2381..2494
                     /gene="rpmJ"
                     /locus_tag="VE170_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYJ15255.1"
                     /translation="MKVRASVKKICNKCKVIRRKGVVRVLCENTKHKQRQG"
     gene            2548..2928
                     /gene="rpsM"
                     /locus_tag="VE170_07180"
     CDS             2548..2928
                     /gene="rpsM"
                     /locus_tag="VE170_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYJ15256.1"
                     /translation="MARIAGVDLPRAKRMEIALTYVYGIGRSTSRNILTAAGVSLDLR
                     SDDLSDDDVAKIRQVLDQSYKVEGDLRREVTGSIKRHLDMGSYRGMRHRKNLPVRGQR
                     THTNARTRKGPRKTVAGKKTAPTK"
     gene            2952..3392
                     /gene="rpsK"
                     /locus_tag="VE170_07185"
     CDS             2952..3392
                     /gene="rpsK"
                     /locus_tag="VE170_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYJ15257.1"
                     /translation="MADAADEKKSEAPAGDAKAADRAARKRRGRKNIAEGVVHIHSTF
                     NNTIVTISDYQGNVISWSSAGAMGFKGSRKGTPFAAQQAADSAAKKAMDHGLRTVQIY
                     VRGPGAGRESALRALQSAGISISLIKDVTPIPHNGCRPPKRRRV"
     gene            3414..4040
                     /gene="rpsD"
                     /locus_tag="VE170_07190"
     CDS             3414..4040
                     /gene="rpsD"
                     /locus_tag="VE170_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYJ15258.1"
                     /translation="MARYIESVCRLCRRENLKLFLKGERCYTDKCAIERRTYPPGQHG
                     QGRPKFSEYSLQLREKQKVKRMYGLLENQFRRTFTEAARTKGITGETLLVLLERRLDN
                     VTYRVGFASSRAEARSLVRHGHILVNGKKVNIPSFTVRVGDVIAVKEPSRQITRVMSA
                     LEGSQRRGVPDWAEVDREAFSGRIKILPTRSDITMPINEKLIVELYSK"
     gene            4072..5085
                     /locus_tag="VE170_07195"
     CDS             4072..5085
                     /locus_tag="VE170_07195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYJ15259.1"
                     /translation="MYKHWTELIKPKQLEVEERTLTATFGRFTAEPFERGFGQTIGNS
                     VRRILLSSLMGASIVAVRIKGVLHEFSTVPGVTEDVAEIILNLKEVRLRLNEGEQATL
                     KIDAKGPGVVSAKDIGGSAAVDILNPDHKIATLSRDGRLDMELLVKLGRGYVPAERNK
                     EESAPVDTIFIDAIFSPVLKVNFTVSNARVGQRTDYDRLVFDIHTDGSVKPDDAMAFA
                     AKILQDQVSIFVNFQDEPRPEKRDDGPSIPLNENLFRSVDELEFSVRSQNCLQNADIK
                     YIGELVQKSEHEMLRTKNFGHKSLNEIKEILREMGLELGMKVDHFPSRAEVEARKRSP
                     DKE"
     gene            5094..5543
                     /gene="rplQ"
                     /locus_tag="VE170_07200"
     CDS             5094..5543
                     /gene="rplQ"
                     /locus_tag="VE170_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HYJ15260.1"
                     /translation="MRHLKSGRQLSRNSSHRWALMRNLITALLREEKIQTTDPKAKEL
                     RRWVERVITLGKRGDLHARRQAASILFDKTVLQKLFDTIGPRFKDRPGGYTRIIKLGV
                     RHGDAAQLSLIELVGNPAGVEAPSAGAKKGRRARRREREQQQQVAAA"
     gene            complement(5737..>5923)
                     /locus_tag="VE170_07205"
     CDS             complement(5737..>5923)
                     /locus_tag="VE170_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15261.1"
                     /translation="SITGLAAVAATSRKISMDSDSSCENIPMDITCSPKLEQFFYGSR
                     FGIDISGIIIENENRAG"
BASE COUNT         1391 a         1538 c         1753 g         1241 t
ORIGIN      
        1 aatggcgagc tcggaccctt ggtcgctttg gtgatattgg ttctcagcgt cgcggtcatc
       61 ggcgcgatta ttttcgtcga gcgcggccaa cggcgcatcc cggtacaata cgccaaacga
      121 gttgtcggcc ggcgcatgta cggcggcatg acctcgcatt tgccgttaaa agtaaacacc
      181 gcgaacgtga ttccgatcat cttcgcttca tcggtgttga tttttccggc gaccatggcg
      241 caattcgcgg cccatccttg gattcaaaca atgtccggaa tgctcgcacc cggtcaatgg
      301 ctctatgaga ttgcccagat cgcgctgatt atctttttcg cattctttta cacggcggtg
      361 gtgtttaatc ccagtgacgt ggccgagaac atgaaaaagg caggcggcta catcccaggc
      421 attagacctg gacagaaaac cgctgagttc attgaaaagg tgttgtcgcg cataaccctg
      481 agcggggcgg tttacctctg cttgatcgcg ttattgcctt ctttgttggt gcgccaattc
      541 aatgtgcctt tttattttgg cggaaccgcg ctgttgatcg tggtgggcgt cgccctggac
      601 accgtggcgc agatggaaac gcatttgatc agcaggaact atgaaggttt catgaagcgt
      661 ggtaggttgc gcggcagaaa gggctaaact tggcggccaa ggcacgtgtg gttctgctgg
      721 gtcctccggg ggcgggtaag ggaacccagg cgaagttgct tgagcaagaa tttggcgcgg
      781 ttcaaatttc caccggagac atccttcgta aagccgtagc cgaccaatcg ccgctcggaa
      841 agcaagctga ggattatatc aagcgcgggg cgttggtacc cgacgatttg atcaccaacc
      901 tcatcgccga acgattgcag caggcggatt gcgctcaggg atttctgctg gacggttttc
      961 cccggacgat acctcaggcc gaaagcctgg atgcgctatt aatgaagttg gcgctgagct
     1021 tgaacggtgt cctgtcggtt caggtgccaa gagcagtcat catcgaacga ctggcgggaa
     1081 ggcggacctg caaaaactgc ggggcgctgt gtcatacggt cttcgatcct ccgaagcaag
     1141 atggagtatg cgaccgctgc gggggcgaat tgtttcaaag ggaagatgat aaggaagaga
     1201 ccatcgccaa ccgtttgacc gtctatgatg gccagaccgc gccgcttgtg gattactatc
     1261 gccgtcaggg tattttgcgg gagatcgacg gggttggcag cattgaggaa atccgttctc
     1321 ggatcaaaaa agccctcgga ggcatagcga cgtgatttcg ctcaaatcgg ctcgagaaat
     1381 cgaaatcatg agaagagcga atgtgattgt tgccgaggtt ctccaggagc tgaaaaggcg
     1441 cgtggcgccg ggcatcacaa ctttggattt ggatgccatt gccgaagaaa tgacgctgaa
     1501 ccgcaacgcg attccggcgt ttaagggcta taacgtcgcc ggccgagttt atccgcgctg
     1561 tttatgcgct tcggtcaatg acgaaatcgt tcacggtatt ccgtcaaacc gccatttgcg
     1621 cgaaggcgat attgtcggcc tcgattatgg ggtcatctat gacggattct acggcgactc
     1681 agcgatgacc gtggcggtcg gcaaagtaag cgcagcggcg caaacgttat tggacgtgac
     1741 cgaggcatcc ctctacgctg gaatcgagca gctccatgag ggcaaacgat tgggcgatct
     1801 cggcgcggcc gtgcaaaagg ttgcagagga tgcgggctat tccgtcgtcc gcgcatttgt
     1861 cggtcatggc atcggcaaga aactccatga ggagccgccg gtgcctaact acggtgaaaa
     1921 ggatcgtggc ttgcgcttaa aagaaggcat ggtgctggcg gtagagccga tggttaatat
     1981 cggcggttac gaagtcgaaa tcaaagaaga cggttggacg gcggtaacca aagatggcag
     2041 cttggccgcc catttcgagc attctatagc gatcactaaa aacggtccgt atattttgag
     2101 tcaactttaa agtttaggag aacacagtaa ggaatcccga tttagctatg gcaaaacaag
     2161 acgccatcga agtcaccggt acggtgctag agaccttgcc caacgccatg ttccgggtgg
     2221 agttggacaa ccaccataag gttttagccc atatctcggg caaaatgcgc atgcactaca
     2281 tcaaaattct tccgggagac aaggtgaaga tcgagctgtc tccctatgat ttggcgcgcg
     2341 ggcgcatcgt cttccgcgaa cgctagcaag aggagaaact gtgaaggtta gagcgtcggt
     2401 taaaaagatt tgcaacaaat gcaaggtgat tcgccggaag ggcgtggtgc gggtgctgtg
     2461 cgagaatact aaacacaaac aacggcaagg ctaagtgttt gtgatattgc aatcggcaat
     2521 ttgaaattcc gaaccaagtg aggatttatg gcacgtatcg caggggttga cttaccgaga
     2581 gccaaacgca tggaaatcgc gctcacttac gtctacggca ttggtcgcag cacatcgcgt
     2641 aatattctca ccgcggcggg agtctcgctg gatttgcgca gtgacgattt gagcgatgac
     2701 gatgtcgcta aaattcgcca ggtgctcgat cagagttaca aagtcgaagg cgatctgcgc
     2761 cgcgaggtga cgggtagcat caaacggcac ttggatatgg gttcctatcg cggcatgcgc
     2821 catcgcaaga atttgccggt gcgtggccaa cgtacccaca ccaacgcgcg cactcgcaag
     2881 ggaccgcgca agaccgttgc cggcaaaaaa accgcgccga cgaaataacc tagataattt
     2941 aggagagact catggcagat gctgcggatg aaaagaagag cgaagcgccg gcgggggacg
     3001 ccaaagccgc cgaccgggcg gcgcgcaagc ggcgcggccg caagaatatc gctgagggag
     3061 tggtccatat ccactcgacg ttcaataaca ccatcgtgac gatttccgac tatcagggca
     3121 acgtgatttc ctggtccagc gccggggcga tgggtttcaa aggctcgcgc aagggcacac
     3181 cgttcgccgc ccagcaagcc gccgacagcg ctgccaaaaa ggccatggat catgggttac
     3241 gcacggtcca aatatatgtc cgaggcccgg gcgcaggacg tgagtcggcg ctgcgcgctt
     3301 tgcagtctgc gggtatcagc atcagtctga tcaaagacgt tacgccgatc ccgcataacg
     3361 gttgccgtcc gcccaagcgc cgtcgggttt gagctttata ggaggaagca ggtttggcta
     3421 gatatattga atccgtgtgc cgactttgcc ggcgcgagaa tcttaagctc tttctcaaag
     3481 gggagcgctg ctataccgac aagtgcgcca tcgaacggcg cacctacccg cccggtcagc
     3541 atggccaggg ccggccgaag ttctcggaat acagcttgca gctgcgcgag aagcaaaaag
     3601 tgaagcgcat gtacggccta ttggaaaacc aattccgtcg caccttcacc gaagccgcgc
     3661 gtaccaaagg aattaccggc gaaaccttgc tggtgctgct ggaacggcga ctcgacaatg
     3721 tcacctaccg ggtgggcttc gccagctcga gagctgaagc gcgctccttg gtgcgccatg
     3781 gccatatatt ggtaaatggc aagaaggtga acataccgtc atttacggtt cgtgtcggcg
     3841 atgtgattgc cgtcaaggag ccgagccggc agatcacccg ggtgatgtcg gcgttggaag
     3901 ggtcgcagcg tcgcggcgtg cccgattggg ccgaagttga ccgtgaagcg ttcagcgggc
     3961 gcatcaagat cctgccgacg cgcagcgata tcaccatgcc gatcaacgaa aaactgatcg
     4021 tagaattgta ttcgaaataa gcagcgaatc ctgggagatg atatgtcgac aatgtacaag
     4081 cattggacgg agctgatcaa acccaaacag ttggaggtgg aggagcgaac tctgaccgcg
     4141 acgtttggac gttttacagc ggaaccgttc gagcgtgggt tcggccaaac catcggcaac
     4201 tcggtgcgcc ggattctctt atcctcgctc atgggcgcct cgatcgtcgc ggtgcggatc
     4261 aagggtgttt tgcacgaatt ctccacggtg ccaggggtca ctgaggacgt tgccgaaatt
     4321 attctaaact taaaagaagt tcgcctgcgg ctgaacgagg gtgaacaagc tacgttgaag
     4381 atcgatgcta aaggtccagg cgtcgtgagc gccaaggata tcggcggatc ggcggcggtc
     4441 gacatcctca atcccgatca taagatcgca acgctctcgc gcgacggcag gctcgacatg
     4501 gaattattgg tcaagctcgg ccgcggttac gtcccggccg agcgcaacaa ggaagagagc
     4561 gcgccggtgg acacgatttt tatcgacgcg attttttcgc cggtgctcaa ggtcaacttc
     4621 acggtctcca acgcgcgggt cggccagcgt accgattacg atcgcttggt gttcgacatc
     4681 cacaccgacg gcagtgtcaa gccggatgac gcgatggcct tcgcggcgaa aattctccag
     4741 gaccaggtga gcattttcgt caacttccaa gatgagccgc ggcccgaaaa gcgcgacgac
     4801 gggccatcga ttccgctcaa cgagaatcta tttcgcagcg tcgacgagct tgaattctcg
     4861 gtgcgttcac agaactgctt gcaaaacgcc gatatcaaat acattggcga gctggtgcag
     4921 aagagcgaac acgagatgct gcggacgaaa aattttggcc ataaatcttt gaatgagatc
     4981 aaggaaatcc tgcgcgagat gggcctggaa ctgggcatga aagtggatca ctttccatct
     5041 cgcgcggaag tcgaagcgcg caagcgcagt cccgacaagg agtagtcgtt accatgcgtc
     5101 atctcaaatc cggacggcaa ctgagccgca attccagcca tcgctgggcc ttgatgcgca
     5161 atctgatcac cgcgctgttg cgcgaggaaa agattcaaac taccgacccc aaggctaaag
     5221 aattgcgccg ttgggtcgaa cgggtcatca ccctcggcaa acgcggcgac ttgcacgcgc
     5281 gccgtcaggc ggcgagtatc ctcttcgata aaaccgtgct tcagaaactt ttcgatacca
     5341 ttggaccgcg tttcaaggat cgcccaggcg gttacaccag gattatcaag ctgggggtgc
     5401 gtcatggcga tgccgcccag ttgtcattga tcgagctggt cggtaacccc gccggcgttg
     5461 aagccccatc ggctggcgcg aaaaaaggtc gccgcgcgcg tcgtcgcgaa cgcgaacagc
     5521 agcaacaagt cgcagccgcg tagcagagtg tcctgacatt cgactttgat cagaccgccc
     5581 ggtctgcagg cgaccctcaa gctggcggac cgttagctgc tcaaccgagc caagtcgggc
     5641 ccgtaagtgg ttgacgtgcc aacggttttg ccccatcaat cttactgtgc gatatgtcag
     5701 tggcgggatc ttgcgcttcg cgtcggactg gcgtatttat ccggcgcggt tttcgttttc
     5761 aatgatgatt ccgctgatgt ctatgccgaa cctcgaacca taaaaaaact gctctaactt
     5821 tggcgaacat gtaatgtcca tgggaatatt ttcacaactt gaatcagaat ccatcgagat
     5881 cttccgcgag gtagcggcga cggctgccaa gccggtgatg ctc
//
LOCUS       DASQKS010000277         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3923835, whole genome shotgun sequence.
ACCESSION   DASQKS010000277 DASQKS010000000
VERSION     DASQKS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3923835"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VE170_07210"
     CDS             complement(<1..1356)
                     /locus_tag="VE170_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYJ15262.1"
                     /translation="MSDIFLSYASEDRATAQKFADALESLGWSVWWDREIPLGKAFDQ
                     VIEEELNAAPCVIVLWSKDAVRSRWVKTEAAAAADRECLIPVLIDDIPIPFEFKRIQT
                     AILMGWQGDAADPEFSPVIVAVRQLLGQSRAPTPLASQSGVKASTGAPIWRVNRGWLI
                     AVAAAAILVGGIIVVKQSREKAAPPLISSNEPVGNSQPPSATVTTVVPGSGKPIASNG
                     AFSIKIGDKIADGVPGPGAGSIETPYSQDVYVFNAAPKQRVYFRQLGHSTGMSYINWQ
                     LTDPDGNKIFNTCFGCTEPGVQTLTKGGQYILTIGNDKDAGTGTYQLQLFDVPAPNQF
                     ALKIGGVIKEGIPGAGAGVIESPGAEDVYTFSASPRQRVYFRIWEQSTGMSYINWKLV
                     DENALEVFNTCLGCSEPAVQQLLKGGTYTLTVGNHKNPSTGTYHAQLYNVPPPHQFAI
                     KI"
     gene            complement(1737..3032)
                     /locus_tag="VE170_07215"
     CDS             complement(1737..3032)
                     /locus_tag="VE170_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15263.1"
                     /translation="MPVSPLLEQAAKRKSLYYGWVIVVVGFLAHIASAFSISSTLAVF
                     LKPLSADLGISRGMFSLIRSGEILIGAAAAPVVGTLMDRYGGRWLMAAGGLISGAGFI
                     CLGQAQGFVQFMLVRWLLISPGDSLMGSMVVNVSISRWFVRMRGRALAIAGMGHGLAK
                     VCMPMAAATLMLYVGWRGAWVVFGVFALLLVVAPALLFMRRSPEEMGLLPDGGPVEVK
                     AQVATPKKPRYAKRTGADDVEWSRSEALRTPVFWLIATTFGVAQIGVTGLNLHVFSYV
                     TDQGHPILVAASVMSIIAIMQFSTPIVWGMLADRMDNGILNMAKCGLQGAGLLVALLL
                     PGMVPLYAGFFIYGIGMGGTAVLAETIWADFFGRLSLGKIRGMGSLVTSIFSACGPPF
                     FGLLFDYTQSYHLSFSIFIAMLFTAAVLSLCLRPPTKSR"
     gene            3267..3656
                     /locus_tag="VE170_07220"
     CDS             3267..3656
                     /locus_tag="VE170_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15264.1"
                     /translation="MKLKLVLTVSSIYLAVLGVGFMFAPRQMGIDAVPADASPALIAY
                     LRIFGGPCLGIAVLNWLARDVEPSAARNAIVLANIVGFGCVTAMDIWAVVSGSGRGAM
                     KLFLVIHLLMTVAFVVAGRARTGAQRR"
BASE COUNT          836 a         1106 c         1131 g          783 t
ORIGIN      
        1 gatcttgata gcgaattggt gcggcggcgg cacgttatac agttgcgcat ggtaggttcc
       61 cgtcgaagga tttttatggt tgccgacggt tagggtgtag gtgccgccct tgagcaactg
      121 ctgaaccgcc ggttcgctgc aaccgaggca ggtattgaac acctccaagg cattttcatc
      181 gaccaacttc cagttgatgt aggacatccc cgtgctttgt tcccaaatgc gaaagtaaac
      241 tctttgccga ggggaggcgc taaatgtata gacatcttca gcgccggggc tctcgatgac
      301 gccggcgcca gcgccgggta tcccttcttt gatcacgccg ccgatcttga gcgcgaactg
      361 gttcggcgcc ggtacgtcaa ataactgtaa ctggtaagtt ccggttccgg catctttgtc
      421 gttaccgatg gtcaggatgt attgacctcc cttggtcagt gtctgcacgc cgggttcagt
      481 acaaccaaaa caggtgttga aaattttgtt gccatccggg tcggtgagct gccaattgat
      541 atacgacatg ccggtgctat ggcctagctg gcgaaaatac actcgctgct tgggcgcggc
      601 gttaaagaca taaacatcct ggctgtaggg tgtttcgata ctgcccgcgc ccggccccgg
      661 aaccccatcg gcgattttat cgccgatctt gattgagaac gcgccgttac tcgcgatcgg
      721 tttgccgctg cccggcacga ccgtggttac ggttgcgctt ggcggttgag aattcccaac
      781 tggctcattc gatgaaatta gcggtggcgc ggctttctct cgtgactgtt tcactacgat
      841 tatgccgcca acgagaatcg ctgccgcggc gacggcgatc aaccaaccac ggttaactcg
      901 ccagattggc gcgccggtag atgcttttac gccgctttgt gatgccaatg gagtaggcgc
      961 ccgagattgc ccaagcagtt gccggaccgc tacgattact gggctgaact caggatccgc
     1021 cgcatcgcct tgccagccca tcagtatggc ggtctggata cgtttgaact cgaagggaat
     1081 cgggatgtca tcgatcaaca ccgggatgag acactcgcga tcggccgccg ccgcggcttc
     1141 ggttttcacc cagcgcgagc gcacagcatc cttggaccac aagacgatga cgcagggcgc
     1201 cgcgtttaat tcctcctcga tgacctgatc gaaggccttg ccgagtggga tctcgcgatc
     1261 ccaccaaacc gaccagccaa gagattctaa cgcgtcggca aatttctgcg cggtggcgcg
     1321 gtcctcgcta gcgtaactga ggaagatgtc gctcatggtt tgactgtgaa tacgcctggt
     1381 aaactcgacc ggcatttact tgtcatcgga cagactatca gtagacaagg accacaccag
     1441 gcgcgctgcg agtcgttggt aatgaaagga gtagcagccg gccgagagtc gttgcattaa
     1501 caagcaggaa agactctcag tacatgtagc cacttgcgcc aacagacctt ggaccaatcg
     1561 gcaagcctgg cgtattaacg ctatctttct tttttttccg cctgacggca agttacctag
     1621 cccgcacctg gtagcgttcg acatcgtcgc ccggttgccg gaaatcgtta cggtcgcgcg
     1681 actttcggat agacataatt tcgatgactt ccttactttc tcgacccaca tccaacttac
     1741 ctagattttg tcggcggccg caggcacaaa ctgagcacgg cggcggtgaa cagcatggcg
     1801 ataaatatac tgaaggacag gtggtagctc tgggtgtagt cgaagagcag gccgaagaac
     1861 ggcggaccgc aggcggagaa gatgctggtc accagtgagc ccatgccgcg aatctttccc
     1921 agcgagaggc ggccgaaaaa gtcggcccaa atggtttccg ccagcaccgc cgtaccgccc
     1981 ataccgatgc cgtagataaa aaagccggca tagagcggca ccattccggg cagtaacagc
     2041 gccaccaaca ggccggcgcc ttgcagcccg catttggcca tattgagaat accgttatcc
     2101 atgcggtcgg cgagcatgcc ccagacgatc ggcgtgctga actgcatgat cgcgatgatg
     2161 ctcatcaccg aggcggccac cagtatcgga tggccttgat cggtgacgta ggaaaacaca
     2221 tggagattga gtccggtgac accgatctgg gcgacgccga aagtggtggc gatgagccag
     2281 aagaccggcg tgcgcagcgc ttcgctccgg ctccactcga catcgtcggc gccggtgcgc
     2341 ttggcgtagc gcggcttttt tggcgttgcg acttgcgctt tcacctccac cgggccgccg
     2401 tcgggcagca atcccatttc ttccggcgag cggcgcatga acagcaaggc cggcgcgacc
     2461 accaacagca gagcgaagac gccgaagacc acccaggcgc cgcgccaacc gacgtaaagc
     2521 atcaaggtcg ccgccgccat cggcatgcag actttggcca agccgtggcc catgccggcg
     2581 atggccaggg cgcggccgcg catgcgcacg aaccagcgcg agatcgaaac gttgaccacc
     2641 atggagccca tgagcgaatc gcccggactg atgagcagcc agcgcaccag catgaactgg
     2701 acgaaaccct gcgcttgacc gaggcagata aagccggcgc cggaaatcaa accgccggcg
     2761 gccatcagcc agcgtccgcc gtagcggtcc atcaacgtgc cgaccacggg cgccgccgcc
     2821 gcgccaatga gaatctcgcc ggaacggatg agcgaaaaca taccgcggga aatgcccaaa
     2881 tcggcggaca acggtttgag aaataccgcc agggtgctgg agatcgaaaa tgccgaagcg
     2941 atgtgggcga gaaagccgac gacgacgatc acccagccgt agtagagaga ctttcttttc
     3001 gccgcctgtt cgagcagagg agagacgggc atctcctgtt tgtagcaaga atcgcggcga
     3061 aactacaagg cgcgcagtca aagcgttgca tgactgaagg agacgagttc tactgcccgg
     3121 cgtttctgcc gaccgcccta gggaaagccg aataattctt ttaaactgaa accaatgaag
     3181 cgcacgctaa agttgaaaac ataatgtgag cgacgaaaat ccgcaatgtt agaacacaga
     3241 cgcgacccaa gacaggagcg tgtcgcatga agctcaagtt agtgctaaca gtctcgtcga
     3301 tttatctcgc cgtccttggc gtcgggttca tgtttgcccc acggcagatg ggtatcgatg
     3361 ccgttccagc ggatgcctcc cccgcgctga tcgcctacct gcggattttc ggcggtccct
     3421 gtcttgggat tgcagttttg aactggttgg ctcgcgatgt ggagccttcg gcggcgcgca
     3481 acgccattgt ccttgccaat atcgtcggct tcggttgcgt aactgccatg gatatatggg
     3541 cggtggtcag tggcagcgga cgcggagcaa tgaagctgtt tctggtgatt cacctgctta
     3601 tgacggtcgc tttcgtcgtg gccggacgcg cccgcacggg agcccaacga cgttaggcca
     3661 agcgatttcg attaacaccc gagctctccc atgacgcggc acgcctgctg tcggctcggg
     3721 gcctctcctg ctatcgcttt gccgagataa ctatggtgaa tggcgggtgc gtactaggga
     3781 atggtgcttt gaaataaagt cagcgatgcc gatcgcgcgt ttcgcgtcag ctctactacc
     3841 gggctcggtg gttaag
//
LOCUS       DASQKS010000278         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5599747, whole genome shotgun sequence.
ACCESSION   DASQKS010000278 DASQKS010000000
VERSION     DASQKS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5599747"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VE170_07225"
     CDS             <1..415
                     /locus_tag="VE170_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYJ15265.1"
                     /translation="KQIAPDPWKAVADKFPVGSKLKGQVTSVPDFGVFVRIDEGVEGL
                     IHVSQLSTERVDKPASLYKVGDEVEAEVVSIDLNDRKIGLSVRALRRSEERQEMENYL
                     KREKEGGRFSFESLLGDELRLDRDDESGRGRKGRN"
     gene            436..1344
                     /gene="sppA"
                     /locus_tag="VE170_07230"
     CDS             436..1344
                     /gene="sppA"
                     /locus_tag="VE170_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HYJ15266.1"
                     /translation="MATLTTKHSILRGLGILFVFILLVFCGTFFYAYLSGGDSKVLSL
                     FSGDGVGVLQVEGAIDDSRFVLAELRRFRETPWIKAVVVRIDSPGGAVAPTQEIFEEI
                     QKTKNKKPCIASMGNVAASGGYYIASACNKILANPGTMTGSIGVIMQLSNFEELMKKI
                     GVKGVNIKSGANKDIGSPFQPLSQEGREILQALVDNVHSQFVTAVAKGRGMDEARVRK
                     LADGRIYSGAQAKDLGLIDQFGTLEDAIELAAKQAGMDGEPAVYHSRPERERWWDRLS
                     MGLFGVRLPASERGWLRYEWSLSMLQ"
     gene            1402..1713
                     /locus_tag="VE170_07235"
     CDS             1402..1713
                     /locus_tag="VE170_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HYJ15267.1"
                     /translation="MTKRDLIDEVNKRFPHLSRRDSEVIINAIFDSMVDALAGGDRIE
                     IRGFGSFVVKNRRARQGRNPKSGALVSVAAKRVPFFKVGKELRLRVDGKPYDPEIDGE
                     D"
     gene            1713..2204
                     /locus_tag="VE170_07240"
     CDS             1713..2204
                     /locus_tag="VE170_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYJ15268.1"
                     /translation="MQPLWAPWRMAYIDDNAKEPGCIFCTKYRQGDLKTALVLTHSRH
                     AVVMMNKYPYNNGHLLIAPKQHENSLARLAPDEYNELSEVLRRAIEIVKTVLNPGGVN
                     VGMNLGKCAGAGIEDHLHWHIVPRWEGDTNFMPVISETRVMPQHLADSYDRLQPHFHN
                     LMV"
     gene            2317..3687
                     /locus_tag="VE170_07245"
     CDS             2317..3687
                     /locus_tag="VE170_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HYJ15269.1"
                     /translation="MKKSGVAVLTVRDEMRKNLVRHLESGERILPGIVGYEDTVIPEI
                     ENAILSGHHMVFLGERGQGKSRIIRGLLSLLDERIPIVDGCEINDSPVAPICSGCRKK
                     AAELGDALPIRWIDRDQRYGEKLATPDVSIADLVGEIDPIKVAEGRYLADEETIHYGL
                     VPRTNRGIFAINELPDLTEKVQVGLFNLMEEKDVQIKGYKIRLPLDVIIVASANPEDY
                     TSRGRIITPLKDRFDVQIRTHYPKKLEDEISIMEQEAVWTDSRSRKVEVPHFMKEILG
                     NLTFEARKSNEINQSSGVSVRVTINNYESLISNAEKRALRCKENDIVPRVSDLHALMA
                     STAGKIEMEYVGEEKKEDELVEKLVNRSIVKVFDQHFSLNSLQKVIEYFNSGWGVEVS
                     DQMASPDYLEGIKEIPGLKEAIKTLGIQESPALMASATEFVLEGLHLHQKLNKEMEGG
                     RVTYGK"
BASE COUNT          906 a         1027 c         1121 g          735 t
ORIGIN      
        1 caagcagatc gctccggatc cctggaaggc ggtggctgat aaattcccgg tgggctccaa
       61 gctcaaaggg caggtaacca gcgtgccgga ttttggcgtg tttgtccgca tcgacgaagg
      121 ggtcgaagga ttgattcatg tctcgcaact cagcaccgag cgagtcgaca agccggcgag
      181 cttgtacaag gtcggcgacg aggtcgaagc cgaagtggtg agcatcgatc ttaacgaccg
      241 caagatcgga ctcagcgtgc gtgccctgcg ccgctccgaa gagcgccagg aaatggagaa
      301 ctatctcaag cgtgagaaag agggcggccg gttttccttc gaatcgctgt tgggcgacga
      361 actgcgcttg gaccgtgacg acgaaagcgg ccgcggccgc aagggccgca actaagcgcg
      421 gcaacggacg cagtcatggc aacgttgaca accaagcatt cgatcctgcg cggcctggga
      481 atattgttcg tcttcatatt gctggtattt tgtgggacgt ttttctatgc ctacttgagc
      541 ggcggcgatt ccaaagttct gtcgctattt tccggcgacg gcgttggggt gttgcaagtc
      601 gaaggagcga tcgacgattc gcgcttcgtg cttgccgagc tccggcgctt tcgcgagacg
      661 ccgtggatca aagcggtggt ggtgcgcatc gacagtcccg gcggcgcagt ggcgccgacc
      721 caggagatct tcgaggaaat ccaaaaaacc aaaaacaaaa agccctgcat cgcatccatg
      781 ggcaatgttg cggcgtccgg cgggtattat atcgccagcg cctgtaataa aatcttggcc
      841 aatcccggca ccatgaccgg ttccatcggc gtcatcatgc agctcagcaa cttcgaagaa
      901 ctgatgaaaa aaatcggcgt caagggtgtc aatattaaaa gcggcgccaa taaagacatc
      961 ggctcgccct ttcaacctct gtcacaagaa ggtcgcgaga tcctgcaggc gctggtcgac
     1021 aacgtgcata gtcaatttgt caccgcggtc gccaaagggc gcggcatgga cgaagcccgc
     1081 gtacgcaagc tcgccgatgg ccggatctat tccggcgctc aagcgaaaga cctcggtttg
     1141 atcgatcaat tcggcaccct agaggatgcc atcgaactag ccgcaaaaca ggccggcatg
     1201 gacggcgagc cggcggttta ccattcccgc ccggaacggg aacgctggtg ggaccgatta
     1261 tccatgggac tcttcggtgt gcgcttgcct gcttccgagc gcggttggct gcgttacgaa
     1321 tggtcgctgt cgatgctgca gtgagcgcgc ttgccggcac agatctaaaa aaaataaaaa
     1381 agctgttgtg agggtgatgt tatgaccaaa agagatttga tcgatgaggt gaataagcgg
     1441 ttccctcatc tctcacggcg ggactcggag gttattatca atgccatctt cgattccatg
     1501 gtcgatgcct tagcgggcgg cgaccgcatc gaaatccgtg gtttcggtag cttcgtcgtg
     1561 aaaaaccgcc gcgcccgtca ggggcgcaat cccaagagcg gcgccttggt atcggtggcc
     1621 gccaagcgcg tgccgttttt taaagtcggc aaagagttgc gcttacgggt cgacggcaaa
     1681 ccgtatgatc cagagatcga cggggaagat tgatgcaacc gctctgggcg ccctggcgga
     1741 tggcttatat cgatgacaac gccaaggagc cgggctgcat tttttgcacc aagtatcggc
     1801 agggcgactt gaaaacggct ttagtgctga cccatagccg ccacgcggtc gttatgatga
     1861 acaagtatcc ctacaacaac ggccatctgc tgatcgcacc caagcagcat gagaatagct
     1921 tggcccgttt agcgccggac gagtataacg aactgagcga ggttttgcgt cgcgccatcg
     1981 agatcgtcaa aacggtatta aaccccggcg gcgtcaatgt cgggatgaac cttggcaagt
     2041 gcgccggcgc gggtatcgaa gatcatctgc actggcatat cgtgccgcgc tgggaaggtg
     2101 atactaattt catgccggtt atcagcgaaa ctcgggtgat gccgcagcac ttggccgaca
     2161 gttacgaccg gctgcagccg cattttcaca atctgatggt ttaagtgtgt tcgagcgacg
     2221 tggtagcttg gcattgccaa gcaataaata gaaactgtca ataactcttc cgaggggagg
     2281 ccaagtccag tgagtgaggc aaaaacatta ggggaaatga aaaaaagcgg agtggcggtg
     2341 ctgactgtgc gcgacgagat gcgcaaaaac ttagtgcgcc atctggaaag cggcgagcgc
     2401 atcctgccgg gaatcgtcgg ttacgaagat acggttatcc cggaaatcga aaatgctatt
     2461 ttgtccggcc accacatggt gtttttgggc gaacgcggcc agggcaagtc gcgcatcatc
     2521 cgcggcctgc tgagcctgct cgatgagcgc atccccatcg tcgatggttg tgaaatcaac
     2581 gacagcccgg tggcgccaat ctgcagcggt tgccgcaaga aggcggcgga gttgggcgat
     2641 gccctgccga ttcgctggat cgaccgcgat cagcgctacg gtgaaaagct cgccacgccg
     2701 gatgtctcga tcgccgatct ggtcggcgag atcgacccga tcaaagtcgc cgaaggccgt
     2761 tatctcgccg atgaggaaac cattcattac ggtctggtac cgcgcaccaa ccgcggtatc
     2821 ttcgccatca acgagctgcc ggatttgacc gagaaagttc aagtcggtct gttcaacttg
     2881 atggaagaaa aagacgtcca gatcaaaggc tacaagattc gactgcccct cgacgtcatc
     2941 atcgtcgcca gcgccaaccc ggaagactac accagccgcg gccggatcat tacgccgctc
     3001 aaggaccgat tcgacgtgca aattcgcacc cactatccga aaaagctcga agacgaaatc
     3061 agcatcatgg agcaggaagc ggtgtggacc gacagccgca gccgaaaggt cgaagtgccg
     3121 catttcatga aggagatcct cggcaacctg accttcgaag cgcgcaagtc caacgagatc
     3181 aaccagtcct ccggcgtcag cgtgcgcgtc accatcaata attatgagag tttgatcagc
     3241 aacgccgaga agcgcgcgct gcgctgcaag gaaaacgaca tcgtgccgcg ggtcagcgac
     3301 ctgcatgccc tgatggcgtc caccgccggt aagatcgaga tggaatatgt cggcgaagag
     3361 aagaaggaag acgagttggt cgagaaattg gtcaaccgct ccatcgtcaa agtcttcgat
     3421 cagcatttca gcctcaactc gttgcagaaa gtgatcgaat atttcaattc cggctggggc
     3481 gtcgaggtct ccgaccagat ggcctcgccg gattatctcg aaggcatcaa ggagatcccc
     3541 ggcctcaaag aagcgatcaa aaccctgggc atccaagaat cgccggcgct gatggcatcg
     3601 gccaccgaat ttgtccttga gggattgcat ctccatcaaa agctcaacaa ggaaatggaa
     3661 ggcggccggg tgacctacgg caagtagcgc aggactagcg cagccaaacc gagtcctcga
     3721 aagataaccc atggtgcata tccgctatag ccgctggtcc gccagcggcg gagactcgct
     3781 ggacccgga
//
LOCUS       DASQKS010000279         4579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1400728, whole genome shotgun sequence.
ACCESSION   DASQKS010000279 DASQKS010000000
VERSION     DASQKS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400728"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..295
                     /gene="rpmF"
                     /locus_tag="VE170_07250"
     CDS             113..295
                     /gene="rpmF"
                     /locus_tag="VE170_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYJ15270.1"
                     /translation="MPVPKRRTSKRVKNQRRAHDHLTAPQWSACANCGETVLPHRACA
                     HCGFYRGRAVIQTENS"
     gene            315..1298
                     /gene="plsX"
                     /locus_tag="VE170_07255"
                     /pseudo
     CDS             315..1298
                     /gene="plsX"
                     /locus_tag="VE170_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
     gene            1426..2358
                     /gene="fabD"
                     /locus_tag="VE170_07260"
     CDS             1426..2358
                     /gene="fabD"
                     /locus_tag="VE170_07260"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYJ15271.1"
                     /translation="MSSEIAFVFPGQGSQSVGMGKALCDQFGAAKQVFAEAEEALGLS
                     LSELCFAGPEADLNLTENTQPAILTVSIAALRVLEAETELRPAYVAGHSLGEYSALVA
                     VGALAFRDAVKIVRARGRLMQQAVPAGQGAMAVILGLEMAAVRGLCFDASENDIVAPA
                     NYNGGGQIVIAGAKQAVVRAMVLAKERGAKRALDLPVSAPFHCELMRPAADGLKQVLQ
                     NVTVNPFTVGVVTNVEAEVNLNNQRVKDLLVDQAVRPVRWEESVNKLSELGCQRALEV
                     GPGKVLKGLIKRIAPALKTDNFETPQDLPGLKAA"
     gene            2360..3106
                     /gene="fabG"
                     /locus_tag="VE170_07265"
     CDS             2360..3106
                     /gene="fabG"
                     /locus_tag="VE170_07265"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HYJ15272.1"
                     /translation="MANLQGQVALVTGGSRGIGRAIALALGQAGAHVVINYKGNQVAA
                     EETLGLLQAADGTGELSVFDIADEVQVEAAVKNIVDAHKNINILVNNAGVTADNLLMR
                     TKSSDWDQVVGTNLKGTILCTKVVSRQMLKQRAGRIVNLSSVVGQTGNVGQSLYAASK
                     AGIIGFTKAMARELASRAITVNAVAPGFIDTEMTARLPEKLREDFLRSVPLGRFGTCQ
                     EVADMVVFLAGPTGSYVTGQVFNVNGGLYM"
     gene            3131..3367
                     /gene="acpP"
                     /locus_tag="VE170_07270"
     CDS             3131..3367
                     /gene="acpP"
                     /locus_tag="VE170_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYJ15273.1"
                     /translation="MAGSIKDRVKEIVCEQLGVSAEEVTPEASFIEDLGADSLDIVEL
                     VMALEEEYEMEISDEDAEKIKTVQDIITYIEAHR"
     gene            3521..4012
                     /gene="rpiB"
                     /locus_tag="VE170_07275"
     CDS             3521..4012
                     /gene="rpiB"
                     /locus_tag="VE170_07275"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HYJ15274.1"
                     /translation="MVGTGDIMIAIGCDHGGVELKDFLVGYLGAKSVEVCDLGTQGRE
                     SVDYPDYGRAVSERVSAGQAERGVLICTSGIGMSIVANKFPGVRAALVVDLDGARSSR
                     EHNDANILVLSGARTAAPLAQAILDTWLTTEFAGGRHQRRVNKITQVEQEFASGTKGQ
                     SKR"
     gene            4032..>4579
                     /gene="glyA"
                     /locus_tag="VE170_07280"
     CDS             4032..>4579
                     /gene="glyA"
                     /locus_tag="VE170_07280"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900862.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYJ15275.1"
                     /translation="MSFLEQVDVDVFAAIERETHRLEDNLELIASENVVSEAVLEAQG
                     SVMTNKYAEGYPGKRYYGGCEFVDEVESLAIARAKELFGAEHANVQPHSGSQANMTVY
                     LAVLKPGDTYLGMNLAHGGHLSMGSPVNFSGFFYNVIPYGVSEKTETIDYDEVERLAK
                     EHRPKLVIAGASAYPRIIDYPR"
BASE COUNT          968 a         1223 c         1439 g          949 t
ORIGIN      
        1 ccggagatcc gcgtatggcg gtgttccgga cgttaaaggt ggggcattga aaattatttt
       61 tattatttta atgtagctgc cgcccgaggc agggaaagcc aggacaaact aaatgccggt
      121 acctaaaaga cgcacatcta aacgggtgaa gaaccaacgc cgcgctcatg accatttgac
      181 cgcgccgcaa tggagcgcgt gcgccaactg cggcgagacg gtactgcccc atcgcgcctg
      241 cgctcactgt ggattttacc gcggccgcgc ggtgatccaa accgaaaata gctgactatc
      301 tttttccgcc agctatgccg aaaatcgccg tcgacgcgat gggcggagac cgagcccccg
      361 gagttgtgat cgaaggggtg ttgctagctc atcaggaact cggcgttgaa atagtcctgg
      421 tcggtccgcc cgacattatc gcgacggaac tcgccaaaca tcccggcggt ggacgtcaaa
      481 tagccatcgc gccggcatcc caagtggtgc cgatgcagga atcccccagc gtcgcgctgc
      541 gcaaaaaaga ctcatcccta cgggtagctt tcgatttgat gaagcgcggc gaagccgatg
      601 cggtggtcag cgccggcaac tccggcgcga tgatggcgct tggcatggtt gtcttgggga
      661 cgctgtcgca ggtggcgcgg ccggcgattc tggttgtcgt tccgagtctg ggagtcggca
      721 cggtaatcgt tgacgccggc gccaatgtcg actgcaaacc caaacatctg ttgcaattcg
      781 gcctcatggg gtcgatttat gcggcacgcg tgcttggcat cgccacgccg cgcgtcggcg
      841 tgttgagcaa tggcgaagag gcgagcaagg gcaccgaatt gacccgtgcg gccagcgtcc
      901 tgctcggcgc cactgggctc aactacattg gctatgtcga gggccgggat atttttaacg
      961 gcaaggtcga cgtcgtggtg tgcgacggct ttaccggaaa cgtcgcgctc aagactatgg
     1021 aaggggtggc gagttttgcc ggcgaaattc tcaaaggcgc ttttcgaaat aatctttcca
     1081 gcaaggtcgg ttacctgatg agccgaaaat cgctgcgcgc ggcttaccgt aaattggatt
     1141 acgctgaata tggcggcgcg cccctgattg ggttggatgg cgtggcgatc gtcgcccatg
     1201 gtggctccag tcccttggcg atcaagagcg cgattcgctt ggcgcgggat gccgtcgatc
     1261 atgagatgaa tcgccatatc gccgaggcct tgggtgatgc ggatggagcc gaagtcgcca
     1321 agaaagaagg tctgtcgcgt acgctctggg atcgcttcaa agcgaaaatc gatgctctcg
     1381 gcgacaaggc ggcgccgagg gagggcgatg accggcggag gagcggtgag tagcgagatt
     1441 gcgttcgtat ttcccggcca gggctcgcaa agcgtcggca tgggcaaggc cctctgtgat
     1501 cagtttggcg ccgctaagca ggtattcgcc gaggccgaag aggctcttgg cttgtccctg
     1561 agcgagttgt gttttgccgg acccgaggcg gatttaaacc tcacggaaaa tacccagccg
     1621 gcgattctga cagtgtcgat cgcggcgctg cgcgtgcttg aagccgaaac cgaactgcgt
     1681 ccggcgtatg tcgccggcca tagtctcggt gaatatagcg ctttggttgc cgtcggcgcc
     1741 ttggcatttc gtgatgccgt gaaaattgtc cgggcgcgcg gccggttgat gcaacaggcg
     1801 gtgcccgccg gccagggcgc catggcggtg atcctcggtt tggaaatggc tgccgtgcgc
     1861 ggcttatgct tcgatgccag cgaaaatgac atcgtcgcgc cggcgaatta caacggcggc
     1921 ggacagatcg tcatcgctgg agctaagcaa gcggtggtgc gcgccatggt cctggctaag
     1981 gagcggggcg ctaagcgcgc ccttgacttg ccggtgagcg cgccgtttca ttgcgagctc
     2041 atgcggcctg cggccgatgg cctaaagcaa gttttgcaaa acgttacggt caaccctttc
     2101 accgtcggtg tcgtgaccaa cgtcgaagcc gaggtcaatc tcaataatca gcgggtcaag
     2161 gatctgctcg tcgatcaggc agtgcggccg gtgcgctggg aggagtcggt taataagctg
     2221 tccgagttgg gctgtcaacg cgccttggaa gtcggtccgg gcaaagtgct caagggtttg
     2281 atcaagcgca ttgcgcccgc gctgaagacc gacaattttg aaacgccgca ggacctaccc
     2341 ggtctgaaag ccgcctgaca tggcaaatct tcaagggcag gtcgcattgg tcaccggcgg
     2401 tagccgaggc atcggcaggg ctattgccct ggcgcttggc caggccggcg cccacgtggt
     2461 gatcaattac aaaggcaatc aagtcgcggc ggaagagacg ctgggcttat tgcaggcggc
     2521 tgacggtaca ggcgaactca gcgttttcga catcgccgac gaggtccagg tcgaggccgc
     2581 ggtcaaaaat atcgtcgacg cccacaaaaa tatcaacatc ctcgtcaaca atgcgggagt
     2641 tactgccgat aatttgctga tgcgcacgaa gtccagcgat tgggatcaag tcgtcggcac
     2701 caatctaaaa ggcacgatcc tgtgcactaa agtggtttca cgccagatgc ttaagcagcg
     2761 ggccggacgc atcgtcaatc tgtcgtcggt ggtcgggcaa acgggcaacg tcgggcagtc
     2821 gctttacgcc gcgtctaaag ccggcattat cggctttacc aaagcgatgg cgcgggagtt
     2881 ggcgtcgcgc gcgattaccg tcaatgccgt cgcgccgggt ttcattgata ccgaaatgac
     2941 ggcgcgcttg ccggaaaaat tgcgcgagga ttttcttcgt tccgttccct tggggcgctt
     3001 cggcacttgc caggaagtcg ccgatatggt tgtgtttcta gccggaccta ccggcagtta
     3061 tgtgaccggc caagtgttta acgtcaacgg cggattatac atgtaaggaa attaagagga
     3121 ggtaagtgag atggcgggat cgattaagga tagagttaag gaaattgttt gcgaacaact
     3181 cggtgtaagc gccgaagagg taaccccgga ggcttcgttt atcgaagatt tgggcgcgga
     3241 ctcgttggac atcgtcgagc tggtgatggc gctggaagag gaatacgaga tggaaatctc
     3301 cgacgaagac gccgagaaga tcaagaccgt ccaggatatc atcacctaca ttgaggcgca
     3361 tcgataaccg ccgttgtccg tacgccgctg cccagtaaac cggccgccgg cgtcgcggac
     3421 tttgtcactg caatatcgta ttcgagtcgc ggacgcgcag gttattcatt gcctgcgctt
     3481 tgtcgtttgc cattttttga atagcgtctg gctaggatct atggtgggga cgggtgacat
     3541 catgatcgcc atcggctgtg accacggcgg cgtggagcta aaagattttc tggttggcta
     3601 cttgggcgcc aagagcgttg aggtttgcga tctcggcacc caaggccggg agtcggtgga
     3661 ctatcccgac tacggcaggg cggtctccga gcgagtatcc gccggccaag ccgagcgcgg
     3721 tgtactgatc tgcaccagcg gtatcggcat gtccatcgtc gccaataaat tccccggcgt
     3781 gcgcgcggca ttggtggttg atcttgatgg cgcgcgttcg agccgggaac ataacgatgc
     3841 caatatactg gttttgagcg gcgccagaac tgcagcacca ctggcgcagg cgattctcga
     3901 tacttggctg accaccgagt ttgccggcgg ccgccaccag cgacgagtga ataagattac
     3961 ccaggtcgag caagaatttg ccagcggcac aaaaggtcag tcgaaacgtt agaatttcca
     4021 gaggagaagc catgtcattt ctcgaacagg tagacgtgga tgtgttcgcc gcaatagaac
     4081 gggaaaccca tcgcttggag gataatctcg aactcatcgc ctcggaaaac gtcgtcagcg
     4141 aagcggtgct ggaagctcaa ggctcggtga tgaccaacaa atatgccgaa ggttatccgg
     4201 gcaagcgata ttacggcgga tgcgagtttg tcgacgaagt tgaatccctg gcgatcgcca
     4261 gggcgaaaga gctgttcggc gccgaacacg ccaatgtcca gcctcactcc ggctcccagg
     4321 ccaacatgac ggtttacctc gcggtgctga aaccgggcga cacctacctc ggcatgaatc
     4381 tcgctcatgg cggccacttg tcgatgggca gtccggtgaa tttttccggt tttttctaca
     4441 acgtgattcc ctacggcgtt tccgaaaaga ccgaaactat cgattacgat gaggtcgagc
     4501 ggcttgccaa agagcaccgg cccaagttag tgattgccgg cgccagcgct tatccacgta
     4561 tcatcgacta tccgcgctt
//
LOCUS       DASQKS010000280         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6041393, whole genome shotgun sequence.
ACCESSION   DASQKS010000280 DASQKS010000000
VERSION     DASQKS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6041393"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1334)
                     /locus_tag="VE170_07285"
     CDS             complement(180..1334)
                     /locus_tag="VE170_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ15276.1"
                     /translation="MKHSTDRILVSHVGSLARPKDLMEMLVARNEGKPYDNVVLAKRT
                     SESVAEVVQKQIDCGIDVVNDGELGKSNFSRYTKERLGGFVEREAGADFKPTSIFGRD
                     LKEFTEYFNRGGRTAIGHHARVFYCVEPLKYIGQDEVNADIANLTAALQGKQYTEGFL
                     PAIAPGTMEHWMKNEYYKTTEEYLFAIATAMREEYKAIVDAGFVLQIDDPDLADAWQM
                     HPEMSLPDYRDYQQLRIDALNHALAGLPEERIRFHMCWGSYHGPHKYDVPLKDIADLI
                     LKVKASAYSIEASNPCHDHEWRVWEEVKLPDGKMLMPGVIGHYSDFIEHPRAIADRLV
                     RYAKIVGKENIIAGTDCGIGSRVGHPQVGWAKFQAMAEGARIASKELWGK"
     gene            complement(1446..2585)
                     /locus_tag="VE170_07290"
     CDS             complement(1446..2585)
                     /locus_tag="VE170_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15277.1"
                     /translation="MYQNKFSIAVTTLLFGIAVLVFPAILFAAEVKPAPWQADWEKTV
                     KAAEEEGAVSIYMTSAFESVFRDGFQKKYPKIKVTSVVGRGFQLGQRVMSERRGEKYI
                     ADLCITGNVTPLTVFHKAKILEPIKPLLVLPEILNEANWFEGKHHYNDPENRYIFVFE
                     GTPRAGDITFNTKLVNAAEIRSYWNLLDAKWKGKIVTIDPLVAGPINTPQIFFYKHPE
                     LGPEFLRRLYAETNMTIVRSDDQLMDWLSTGRHALGFGARDVDKAMAQGLPINQFVPN
                     HMKEGASLATYNGTLSFFNRAPHPNAAKVAINWLLSKEGQSTWIEHNAKTFGQYDSLR
                     EDIPKDKVLDLAKRSKGGRYLWIKTEWIDEIDTIRDLVKKALPAK"
     gene            complement(2666..4156)
                     /locus_tag="VE170_07295"
     CDS             complement(2666..4156)
                     /locus_tag="VE170_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HYJ15278.1"
                     /translation="MNTRAAMKHPARTAQFEELRSIDLQRCESVGDIVDAMRYCAFGA
                     RMLGEAAKTICEMASAPDKPLLIYDGLENTPLGRLLKKFVKKRWCRRMVTPSAYAKQK
                     TRGGNVIVVGAFSERDAEAVYKKPKRAIFINPFDMARPGQVRDGFFPDAVFADPRFIM
                     PVIYAALDEWLNGRRSSITSFISGLAANGGLGAQVARGANALEAMVGDKDCVRFLTVS
                     GAMTVGKMDLVICDMIELGLIHAISSTGALMAHGLVSSIGLKHYKYNPKYDDTDLARL
                     KLNRVTDTLEPETNLDTVEEVIGTVIDKLDGKQMLSPTAFNKLIGKHLAENYPNDRGV
                     LKSAYLHGVPVFVPAFVDSELGNDIYIHNIKRRRRGKKPILMDLELDSKELIRVVTGP
                     KRFGILSIGGGVPRNNVQNVAPLIEIINERLGPTFPNRRFHYGVRICPDKPHFGHLSG
                     CTYSENESWRKADKNGIYAEMLADATQVWPFLVKYVMEQKAAIAKR"
     gene            complement(4246..>5026)
                     /locus_tag="VE170_07300"
     CDS             complement(4246..>5026)
                     /locus_tag="VE170_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15279.1"
                     /translation="PLRIAEEFAMVDQLSAGRLCFGAGRGMHPTEYAVFGADWSNAQK
                     RLPEALDIVMRAWSGEEFEWQSEHYKFPKIRVYPQPYQRPHPPIYITANRDPESFHLV
                     GSRGHHLMTLPWIATNEEQGPRVESYKAALRGAGHAVDDKDVFTMYPVYVGDSDEQAK
                     ADVVDHWHRWRSFALDAVGATPGTPAYERIFPHLSYDAMVRDSRGVFGGPASCVRILK
                     RIVETVGTTHIGLTFHFGGLSQGKTLLSMERCANSVLPALR"
BASE COUNT         1005 a         1460 c         1422 g         1139 t
ORIGIN      
        1 gaagagcgcg gccgcatcga agagcgcttg cgtaatgtgc cgccgtcgga agaccgcgag
       61 atcctggtgc gagaatacgg ccgccaggtt ttaggcgtga agtaagtcga tcgatcgccg
      121 gaggacgaag caccgcagca atgtgcgcgc ttcctcctcc ggcttgaccc cgtctttttt
      181 tactttcccc acaattcttt ggatgcgatg cgcgcccctt cggccatcgc ttgaaacttc
      241 gcccagccga cctggggatg gccgacccgt gagccgatgc cgcaatcggt gccggcgatg
      301 atgttttcct tgccgacgat tttagcgtaa cgcaccaagc ggtcggcgat cgcccgcgga
      361 tgctcgatga agtcgctgta atggccgatg acgccgggca tgagcatctt gccgtccggt
      421 agcttcacct cttcccagac acgccattca tgatcgtggc agggattgga tgcctcgatg
      481 gaataggcgc tcgcttttac tttgagaatc agatcggcga tgtccttgag cggcacgtcg
      541 tatttgtgcg gcccatggta actgccccag cacatgtgga agcgaatacg ctcttcaggt
      601 aagccggcga gtgcatggtt gagggcgtcg atgcgcagct gctgataatc gcggtagtcc
      661 ggcagcgaca tttccggatg catctgccag gcatcggcga gatcgggatc gtcgatctgt
      721 aagacaaagc cggcgtcgac gatcgccttg tattcttcgc gcatcgctgt ggcaatggcg
      781 aaaagatact cttcggtggt tttgtaatat tcgttcttca tccaatgctc catcgtgccg
      841 ggagcgatgg ccgggagaaa tccctctgtg tactgcttgc cttggagcgc cgctgtcaaa
      901 ttggcgatgt cggcgttgac ttcatcctgg ccgatatatt tgagcggctc gacgcaatag
      961 aacacgcggg cgtgatggcc gatggcggtt ctgccgccgc ggttgaaata ctcagtgaac
     1021 tctttcaggt cgcggccgaa gatcgacgtc ggtttgaaat ccgccccggc ttcgcgctcg
     1081 acgaaaccgc ccaaccgctc tttggtatag cgcgagaagt tcgatttgcc caattcgccg
     1141 tcgttgacca cgtcgatgcc gcagtcgatc tgcttttgca ccacttcagc caccgactcg
     1201 ctggtccgct tggccagcac cacgttgtca taaggcttgc cctcattgcg cgccaccaac
     1261 atctccatca gatctttagg ccgcgccaaa ctgccgacat gcgagactaa aattctatcc
     1321 gtgctatgtt tcataaatcg ttctcctcta gcaaaaagtt ggagtaatgg aatggtggag
     1381 tattggagcg atggttgttc ggaatgccca ttactccagt actccaatac tccaattttt
     1441 tttgtctatt tcgccggcag cgccttcttg accaaatccc gaatcgtgtc aatttcatcg
     1501 atccactcgg ttttgatcca cagatagcgc ccgcctttgc tgcgcttggc gagatcgaga
     1561 actttatcct tgggaatgtc ttcgcgcagg gaatcatact ggccaaaagt cttggcgttg
     1621 tgctcgatcc aggtgctctg cccctctttc gacagcagcc aattgatcgc caccttggcg
     1681 gcgttcggat ggggtgcgcg gttgaagaaa ctcagcgtgc cgttgtaagt cgcgagcgac
     1741 gcgccttcct tcatgtggtt gggcacaaat tggttgatcg gcaatccctg ggccatcgct
     1801 ttgtcgacat cgcgggcgcc gaagccgagg gcgtggcgcc cggtgctgag ccagtccatc
     1861 agctgatcgt ccgagcgcac aatcgtcatg ttggtctcgg cgtagagacg gcgtagaaat
     1921 tccggaccga gctcgggatg cttgtagaaa aaaatctgtg gcgtgttgat cggcccggcc
     1981 accaatggat cgatcgtgac gattttgcct ttccatttgg catcgagcag attccagtaa
     2041 gagcgaatct ccgccgcgtt gaccagcttc gtattaaatg tgatatcgcc cgcccgcggc
     2101 gtgccttcaa agacgaagat ataacggttc tccggatcgt tgtaatgatg cttgccctcg
     2161 aaccaattgg cttcgttcag aatctcgggc agcaccaaca gcggcttgat cggctcgagg
     2221 atcttggcct tgtgaaacac cgtcagcggc gtgacattgc cggtaataca cagatcggcg
     2281 atgtacttct cgccgcgccg ctcgctcatc acccgttggc ccagttgaaa gccgcggccg
     2341 actacggatg taaccttgat cttgggatac ttcttctgaa acccatcgcg aaaaaccgac
     2401 tcgaacgccg aggtcatgta aatgctgacc gcaccctctt cctccgcggc tttcacggtc
     2461 ttttcccaat ccgcttgcca gggcgccggc tttacttcgg cggcgaataa aatcgcaggg
     2521 aatactaaga ccgcaatgcc aaagagaaga gtggtcactg cgatggaaaa tttattttga
     2581 tacatattaa atccaaagcc cgtcaatccg ctttgtcaaa ttggcatgtt cggacgttcc
     2641 ggaaaaatat tgcaccggca caactttacc gcttcgcgat cgcggctttc tgctccatta
     2701 cgtacttcac caaaaacggc cacacctgag tcgcgtcggc gagcatctcg gcgtagatgc
     2761 cgttcttgtc agccttgcgc cacgactcat tctcggaata ggtgcagccg gagagatggc
     2821 cgaagtgcgg cttgtccggg cagattctca cgccatagtg gaatcggcga ttgggaaagg
     2881 tcggccccag ccgttcgttg atgatttcga tcaacggagc gacgttttgc acgttgttac
     2941 gcggcacgcc gccgccgatg gaaagaatgc caaaccgctt cggcccggtg accacgcgaa
     3001 tcaactcttt gctgtcgagc tccaagtcca tcaggatcgg tttcttgccg cgccggcgcc
     3061 gtttgatgtt gtgaatgtag atatcgttgc ccagctccga gtcgacgaag gccggcacaa
     3121 aaaccggcac gccgtgtaaa tacgccgact ttaagacgcc gcgatcgttg ggataattct
     3181 cggccaagtg cttgccgatc aatttgttga aagccgtcgg gctgagcatt tgcttgccgt
     3241 cgagcttatc tatgaccgtg ccgatcacct cttcgacggt gtccagattg gtttccggct
     3301 ccagggtatc ggtcacgcgg ttgagcttga gccgcgccag gtcggtatcg tcgtacttgg
     3361 gattgtactt gtagtgtttg agaccgatgg acgataccaa gccatgagcc atcagcgcgc
     3421 cggtggagga gatcgcatgg atcaacccca actcgatcat gtcgcagata accagatcca
     3481 tcttgccgac ggtcatcgcg ccgctaaccg tgagaaaacg cacgcagtct ttatcgccga
     3541 ccatcgcttc aagagcgttt gcgccgcgcg ccacctgcgc tcccaatccg ccgttggcgg
     3601 ccaggccgga gatgaaggaa gtgatcgagc tgcgcctgcc gttcaaccat tcatcgaggg
     3661 cggcgtaaat caccggcatg atgaagcgcg gatcggcgaa cacggcgtcc gggaaaaacc
     3721 cgtcgcgcac ctggccgggc cgcgccatat cgaagggatt gataaagatc gcccgcttgg
     3781 gtttcttgta gaccgcctcg gcatcgcgct cggagaacgc accgacgacg atgacattgc
     3841 cgccgcgtgt cttctgcttg gcgtaggccg acggcgtcac catccgccgg caccagcgct
     3901 tcttgacgaa tttcttcagc agccgcccga gcggcgtatt ctccaggccg tcgtagatca
     3961 gcagcggctt gtctggcgcc gatgccattt cgcaaatggt tttggctgct tcgccgagca
     4021 tgcgcgcgcc gaaggcgcag tagcgcatgg cgtcgacgat atcaccgacc gattcacacc
     4081 gttgcagatc gatggatctc agttcctcaa attgtgctgt cctggcagga tgtttcatgg
     4141 cggctcttgt gttcatcaaa tgtccatagc agagatggcg ggttttagac ccacggtaca
     4201 agacaaaatc tttttttgta ttctttgcgc taaccttccg ggattctagc gcagcgccgg
     4261 caacaccgag ttggcgcagc gctccatcga caggagcgtc ttgccctggc ttaagccgcc
     4321 gaagtgaaac gtcaagccga tatgcgtcgt gccgacggtc tcgacgatac gcttgaggat
     4381 gcgcacgcag ctcgccggcc cgccgaacac gccccggctg tcgcgcacca tggcgtcgta
     4441 agatagatgc ggaaatatgc gttcgtaagc tggcgtgccc ggtgtagcgc cgaccgcgtc
     4501 caacgcgaag ctgcgccaac gatgccagtg atcgacgaca tcggctttgg cttgttcgtc
     4561 gctgtcgccg acatagacgg gatacatggt gaacacatct ttgtcgtcga cggcatggcc
     4621 cgccccacgc agcgcggctt tgtacgattc cacccgcggc ccctgctcct cgttggtggc
     4681 gatccacggc aaggtcatca aatgatggcc gcgcgagccg accaggtgga aactttccgg
     4741 atcgcggttg gcggtgatgt agatcggcgg atggggccgc tgataaggct gcgggtagac
     4801 tctgatctta ggaaatttgt aatgctcgct ctgccactcg aactcttcgc cgctccacgc
     4861 ccgcataacg atatcgagag cctcgggcaa tcgcttctgt gcattgctcc aatcggcgcc
     4921 aaacaccgca tattcggtcg gatgcatgcc gcgcccggcg ccgaaacaga gccgccctgc
     4981 ggacagctga tcgaccatgg cgaactcttc agcgatgcgc agcggg
//
LOCUS       DASQKS010000281         8368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5179132, whole genome shotgun sequence.
ACCESSION   DASQKS010000281 DASQKS010000000
VERSION     DASQKS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5179132"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..2056)
                     /locus_tag="VE170_07305"
     CDS             complement(308..2056)
                     /locus_tag="VE170_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ15280.1"
                     /translation="MAAAHSPRSGAGFRPPLLSAGGIVLLGLSVILVYQVAVPLGLII
                     WTSLKTIRPGEAGFLDFSFTLANYSRAYGIGEFWRASLNTLYFALATTVLAFIWGTFL
                     AWTVERTNTPLARLIGLVTLGRIIIPGVIITVSWILMASPSIGILNHLIASMTGVSRF
                     LNIYSFWGMVWVQALEMTPLAYLLMSAALQSIDPRLEEASAIAGGGTWPTFRRITLPL
                     VLPAAAAAALLLFIQTVESFEVPLLLGGRARVSVYTTEVYFNTSRMPTDWGLSSTYSM
                     VLLLLAMVLLFVYFRLVRHGERYQTITGKDYRPRRVDLGRWKYPACALSLLLIFLITG
                     LPFLTMLYASFLPRFQAPTAEAFQAMTWMNYANLLADDGYALKPLWNSTLLGVGSATA
                     VMLLVAGISYFVHKTRIRGRKILDFLGFAPIALPSVVLGSAFMWLYLIIPLPVIGTLS
                     IIGLAYLTRYLPFALRFVSTAMVQIHAELEEAAAVAGGGWWRNFYHIYLPLLRPGLMA
                     GWFWVMVHAYRELTISLMLARSNNRTASVIIFDLWSNGSFQQLSAFGVVMFTLLIVLV
                     AIAQKISRRYGIQEQV"
     gene            complement(2075..3151)
                     /locus_tag="VE170_07310"
     CDS             complement(2075..3151)
                     /locus_tag="VE170_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15281.1"
                     /translation="MTSLVRISIVVVAMVIVSWGEDSRGAERATEQLYATLAKLPADQ
                     REKRIEEGARKEGKLNFVHTWRGKNARDHVKLFEKRYPFVKVDMGDLGSQDAAERFIA
                     EESAGRHLTDILTLGVPDMAIILKHNFVAKFPTPATKKILKQYQNFIDPAERWIPWYW
                     AEHGISYNTNLIKPDKAPKTWFDLCKPEFKGQVSFDPSETRFLAGLYVMMGEEKTKEW
                     LKCMGQNQIIIQTGHTQRMELMLTGDHALQGDNYFYTGVEAKRKDPKAPFAMVLTAPI
                     LSFAGGMVINKNALNPYASALFVDWTLSPDSQNMINEILRGPLTIKHPYLPDNVKLVT
                     YNIVSDDITKRLHDAWGQYIGRMK"
     gene            3264..4220
                     /gene="argC"
                     /locus_tag="VE170_07315"
     CDS             3264..4220
                     /gene="argC"
                     /locus_tag="VE170_07315"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HYJ15282.1"
                     /translation="MPAKIYIDGQAGTTALRIRDWLAGRGDLDVVTLPEELRKDPATR
                     KKALQDASIVLLCLPDDAAKEAAAWLADSPVRILDASTAHRVVDGWVYGLPELVAGQR
                     EQITQAKRVANPGCYAAAVILLTRPLVDTGLIAAGAPLAIHALSGYSGGGRALIERWE
                     DPKKKLIHLPHEAPYSIDKLHKHIPEIMRHGALNTEPQFLPNVGPFRCGMRVQIMIQA
                     NSLPKKGSAKAMWEALAARYDKEAFIEVEPFTDAGETDETSFDPQEFNDTNRISLRVL
                     PHQSGHVLLMARLDNLGKGAAGVAIQNLNLMLGVPEGTGLPR"
     gene            complement(4309..5550)
                     /locus_tag="VE170_07320"
     CDS             complement(4309..5550)
                     /locus_tag="VE170_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15283.1"
                     /translation="MWYSIPLTLFWFTYFGSLGVFYPYFTLYLRENAGLTGTQVGMVL
                     AISPLIGMFAQPLWGQVADRTGARTRVLAFLTLGTALGYLGLGYAQQFGAILLATAAL
                     ALVGTAVFPLMTSVSLAILRDSGRHAFGRVRGWGTIGYFILVLVFPWALTLYQPSAEQ
                     SSIASANVSQPGMGLMFPLTASLVFVAALIAFLLPQKGAVGWRAARGDWRELLRNRAF
                     FRFLCFSLVGHFLMHGPMWLFPIFIRARGGDLTTIRNMWILMLIVEVPLVFLTGSGLK
                     RLGARGLLTVGVTVGGLRWILSALITDPMVLFAVQALHGVTVVGFNLGSPLYLDVVAP
                     EKLRSTAQGILSMVGNGLAGILSNLTAGWLVDRSGTDALYLLCGIGSLTLGISSRWIL
                     PKVERRREAPKEIVLASETLT"
     gene            complement(5732..6427)
                     /locus_tag="VE170_07325"
     CDS             complement(5732..6427)
                     /locus_tag="VE170_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ15284.1"
                     /translation="MNDKATAIVIDDEGQIRKLLRVVLEEHGYDVVEAETGKSGLAEI
                     ATRGPDVVLLDLGLPDMDGIEILKRLREWSQVPVLILSVREAAEQKVAALDAGADDYV
                     TKPFDATELLARLRAIQRRGQEGEANAYFQTGNLAIDFSGHIVTVNGREVKLTATEYD
                     LLRVLALNAGKVVTHSHLLREVWGPNATEQSQYLRVYVNHLRKKIELPRSKEKLIKTE
                     SGIGYRLMPADAK"
     gene            complement(6424..>8368)
                     /locus_tag="VE170_07330"
     CDS             complement(6424..>8368)
                     /locus_tag="VE170_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ15285.1"
                     /translation="GGPWKSSERLMVAVGGDAGSESLIRWTRRVAGEFGCSWLAVYVD
                     PDRAVGSENKDQLTRSLSLVRQLGGEVVLTTGNDRSEALLRIARQQNVTQIIVGKPAV
                     AGGWGWLKGRWLLNRLIHRSGAIDVYVVQADRLVVAPTRSGAARTMQSPLHEFLIAAL
                     GVAGVTVISLGFEPITGYLAVALLYLLLVVVLGVRLGRAPVFAAATLSALLWNFLFIP
                     PQLTFYIGKLHDVMMFAMFFIVALAMGHLTGRLHQSQIAERKRERRTAALYELAHQAA
                     FAPELDAGLRAAVAHIESIMDAKAALVLRQSDHTLDNTVNPASSFALSEKEQSVAAWA
                     FSRRAPAGRFTDTLPDAEALYLPLQARTAAMGVLCIRLPMDRALDLSERDLLEAFAVL
                     IGLLLEKDHIAAAIQRAEILEASERLRRSILDSVSHELKTPLAAVQTGLAALEQQSKN
                     DLAQTETVGEIKFATRRLNRVIGNLLEMTRIETGVVQPKLDWCDIEEVVEGAVALAAE
                     ALSRHPLEVDIKENLPMIKIDHLLIEQSLANLLANAANWSESGKTITVSADIGDNELR
                     LIVADRGPGIPQEELPHVFEKFYRGAKAPSGGTGLGLSIVEGFIRAHGGSVHAANRPG
                     GGAEFTIRVPAEALNEKNMELAD"
BASE COUNT         1908 a         2491 c         2381 g         1588 t
ORIGIN      
        1 cccagaagac cagaacgcgt tcgacttttc gctcaatcat gctgtcgacg gcctgcggag
       61 atatcgaatc aaccaggaac atttgtttaa ggtgacccgg tgggagtgga ttgaagcgta
      121 acaagtcgtt taggtgacgt cggtagtttc gtaggatggg ttgacttcag cgatacccat
      181 cgttcttgga cggacaaact caatgcgctt aattcgtagc tgcgctccct aactgaacct
      241 agggctccgc cgccagccga ccctcagcgc cggtctgcac cttgacccgc ggtaaaacta
      301 tttttgttta aacctgctcc tgtatgccat accgtctgct gatcttctgc gcgatggcca
      361 ccagcacgat cagcaaggtg aacatgacga cgccgaaggc gctcaactgt tgaaaggaac
      421 cgttcgacca caggtcgaag atgatcaccg aggcggtgcg attattcgag cgcgccagca
      481 tcagcgaaat ggtcagctcg cggtacgcat gcaccatcac ccagaaccat ccggccatga
      541 gtccgggacg aagcaggggc agatagatat gataaaaatt tctccaccag ccaccgcccg
      601 cgaccgccgc ggcttcttcg agttcggcat ggatttgcac catggcggtc gagacgaagc
      661 gcaatgcgaa cggtaaatat cgcgtcagat aagccaagcc tataatgctc agcgtgccaa
      721 tcaccggcag cgggatgatc agataaagcc acatgaacgc cgacccgagc acgacgctcg
      781 gcagcgcaat tggcgcaaag ccaaggaagt cgagaatttt ccgtccgcgg atgcgcgtct
      841 tatgcacgaa atagctaatg ccggcgacca gcagcatcac cgcagtcgcc gaaccgacgc
      901 cgagcagcgt gctgttccat aggggtttca aggcatagcc gtcgtcggcg agcaggttgg
      961 cgtagttcat ccaggtcatc gcctggaagg cttcggcggt cggcgcttgg aaacgcggca
     1021 agaacgacgc gtagagcatc gtgagaaacg gcaacccggt gatgagaaaa atcaataaca
     1081 agctgagcgc gcaagccgga tatttccagc ggccaagatc aacgcgtcgc ggccggtagt
     1141 ccttgccggt gatggtttgg taacgctcac cgtggcgcac cagtctgaaa tagacgaaga
     1201 gcaacaccat cgccaagagc agcaacacca tcgaataggt gctcgataac ccccagtcgg
     1261 tcggcatgcg cgaggtgttg aagtaaactt ccgtcgtgta gaccgacacg cgcgcgcggc
     1321 cgccgagcaa tagcggcacc tcgaagctct ccacggtttg gataaacaaa agtaaagccg
     1381 cggcggccgc ggccggcaag accagcggca aagtgatcct gcgaaaagtc ggccaagtgc
     1441 cgccgccggc gatcgccgat gcctcctcga gccgcgggtc gatcgactgc aaggccgccg
     1501 acattaacaa ataagccagc ggcgtcattt ccagcgcctg cacccagacc atgccccaga
     1561 acgaataaat gttgaggaat ctgcttacac cggtcatcga agcgatgagg tgattcaaaa
     1621 ttccaatgct cgggctcgcc atcagaatcc acgagaccgt gatgatcaca ccaggaataa
     1681 taatccggcc gagggtgacc aggccgatca gccgcgccag cggcgtgttg gtgcgctcga
     1741 cagtccaggc gagaaatgtg ccccagataa atgccaaaac agtcgtcgcc agggcgaagt
     1801 aaagcgtgtt gagactcgcg cgccaaaact cgccgatgcc gtaggcgcgg ctgtaattgg
     1861 ccaaagtaaa actgaaatcc aaaaagccgg cctcgccggg acgaatggtt ttgaggctcg
     1921 tccaaatgat tagccccagc ggaaccgcaa cttggtagac gagaataacg ctgagaccga
     1981 gcagcacgat gccgccggcg ctcagcaaag gcggacggaa gccggctccc gagcgcggtg
     2041 aatgggcggc ggccatggta ggaaatgagg caggttattt catgcggccg atatactggc
     2101 cccaggcgtc atgtaaacgc ttggtaatat cgtcgctgac gatattatag gtcactagct
     2161 tgacgttgtc gggcagataa gggtgcttga tcgtcagcgg gccgcggaga atttcattga
     2221 tcatattttg actgtcaggc gatagcgtcc aatcaacgaa tagcgccgac gcgtaggggt
     2281 tcagggcatt cttgttgata accatgcccc cggcaaacga caggatcggc gccgtcagga
     2341 ccatcgcaaa aggtgccttg ggatctttgc gcttggcttc gacgccggtg tagaaataat
     2401 tgtcgccctg tagcgcgtgg tcgccggtga gcatcagctc catgcgctgg gtgtgaccgg
     2461 tttggataat gatttgattc tgtcccatgc acttgagcca ctctttggtc ttctcctcgc
     2521 ccatcatgac atacagcccg gcgagaaacc gcgtctccga cggatcgaag gagacttgcc
     2581 ctttgaactc cggcttgcac agatcgaacc aagtcttggg cgctttgtcc ggcttgatca
     2641 aattggtgtt gtaggaaatg ccatgctcgg cccagtacca ggggatccag cgctccgccg
     2701 gatcgatgaa gttttgatac tgctttaaga tctttttggt tgccggcgtg ggaaatttgg
     2761 cgacgaaatt gtgtttgagg atgatcgcca tgtcgggaac gccgagggtc aggatatcgg
     2821 tcaaatggcg cccggcgctc tcctcggcga tgaaacgctc ggcggcgtct tgcgagccca
     2881 agtcgcccat gtcgactttc acgaatggat agcgtttctc gaacagcttg acgtgatcac
     2941 gcgcgttctt gccgcgccaa gtgtggacga aattgagctt accctccttg cgcgcgcctt
     3001 cttcgatgcg tttttcccgc tggtcggcgg gaagtttcgc cagagtggcg tagagctgct
     3061 cggtggcgcg ctccgcgccg cggctatcct cgccccatga cacaatgacc atagcgacga
     3121 cgacgatcga gattcggacc agacttgtca tagagccctc ctgtagctcg caccggttgt
     3181 gattcgtata tgtcaggtgg ctgcgccatt tcaagttaat aaaattctgc taagatttat
     3241 ccaacagcaa gaaagacttt cgagtgccag ccaaaatcta catagatggc caagcgggga
     3301 ccaccgcgct gcgtatccgc gactggctcg ccggtcgcgg cgatctcgat gtcgtcacgc
     3361 ttcctgagga gttacgcaaa gatccggcga cgcggaagaa ggcgctgcaa gacgcaagca
     3421 tcgtgctgct ctgccttccg gatgacgcgg caaaagaagc ggcggcatgg ctcgccgact
     3481 caccggtgcg aatccttgac gccagcacgg cgcatcgggt ggtcgatggc tgggtttatg
     3541 gcctgcccga actggtggcc gggcagcgcg aacagatcac tcaagcgaag cgcgtggcca
     3601 atcccggctg ctacgccgca gccgtgattt tacttacccg gccgctcgtc gataccggct
     3661 taatcgccgc cggcgcaccg ctggcgatcc acgcactatc gggctactcg ggcggcggcc
     3721 gggcgttgat tgagcgctgg gaagatccga agaagaaatt aatccatttg ccccacgagg
     3781 cgccctacag catcgacaag ctgcacaagc atattccgga gatcatgcgc catggcgcat
     3841 tgaatactga accgcagttt ctccccaacg tcggaccgtt tcgctgcggc atgcgcgtgc
     3901 aaatcatgat tcaggcgaat tcactaccaa aaaaaggctc ggccaaggcg atgtgggaag
     3961 cactggccgc gcgttacgac aaagaagctt tcatcgaggt cgaaccgttc accgacgccg
     4021 gcgagactga cgaaaccagc ttcgatccgc aagagttcaa cgacaccaat cgcatttcgc
     4081 tgcgggtctt gccgcatcag tccggccatg tccttctgat ggcgcgcctg gacaatctcg
     4141 gcaaaggcgc cgccggcgtg gcgattcaga atttgaatct catgctcgga gtcccggaag
     4201 gcacgggact accgcgctaa gcgactcacc tcgctcgtga gtcgtcctga gcgccagcga
     4261 aggatcttgg cttagtgaca caattttagg ttcagcaggc cttccgcact aagtaagcgt
     4321 ctcactggcg agaacgattt ccttcggcgc ctcccgccgc cgttcgactt tcggcagaat
     4381 ccagcggctc gaaatcccca gcgtcagcga accgataccg cacaacaggt agagcgcgtc
     4441 ggtgccgctg cgatcgacca accaccccgc cgtcaggttc gataggatcc cggccaagcc
     4501 gttgccgacc atggagagaa tgccctgggc cgtcgagcgc agtttctccg gcgccaccac
     4561 atccaagtag agcgggctgc caagattgaa gccgacgact gtgacgccgt gcagcgcctg
     4621 caccgcgaac aagaccatcg gatcggtgat gagcgccgaa aggatccacc tcagaccgcc
     4681 gacggtcacg ccgacggtca acaaaccgcg ggcgcccagc cgtttcaagc cgctgccggt
     4741 gagaaagacc aacggcactt cgacgatcaa catgagaatc cacatattgc ggatcgtggt
     4801 taaatcgccg ccgcgggcgc ggataaaaat cggaaacagc cacatcggcc catgcatgag
     4861 gaagtggcca accagggaaa aacaaagaaa gcgaaagaag gcccgattgc gcaacaactc
     4921 gcgccaatcg ccgcgggcgg cgcgccagcc caccgcgcct ttctgcggca gcaagaacgc
     4981 gattaacgct gcgacaaaaa ctaacgaagc cgttagcgga aacatcaggc ccatgccggg
     5041 ctgtgacacg ttcgcactgg cgatggagga ttgctctgcc gacggctgat agagcgtcag
     5101 cgcccaagga aagacgagaa ccaggataaa gtaaccgatg gtgccccagc cgcgcacccg
     5161 cccgaacgcg tggcgcccgg aatcgcgcag gatcgccagg ctcaccgaag tcatcagcgg
     5221 aaataccgcg gtgccgacga gggccagcgc cgctgtcgcg agcaggatcg cgccaaactg
     5281 ttgcgcatag cccaaaccaa ggtagcccag cgccgtgccg agcgtgagaa acgccaacac
     5341 ccgcgtgcgc gcgccggtcc ggtccgccac ctgcccccac aatggctgag caaacatgcc
     5401 gatcaacggc gagatggcca acaccatgcc gacttgagtg ccggtcaaac cggcgttctc
     5461 gcgcagatat aaagtgaagt aaggataaaa aacaccgagg gaaccgaaat aggtaaacca
     5521 gaaaagagtc agcgggatcg aataccacat tcggcgatga tagcattcat cgtcgccgtc
     5581 tacgactccg gtaccaactg ctgcaatggt tttcggcaga aacgatttga acggcttgaa
     5641 cgttttgagc gattggaact cttcgaagaa tttacaaccc gagcggagcg aactaaatta
     5701 ccgcgatcaa gatctatggt gaggccgcca gctatttggc gtcggcaggc atcaatcggt
     5761 agccgatgcc gctttcggtc ttgatcagct tctctttgct gcgcggcagt tcgatctttt
     5821 tgcgcaagtg gttcacgtaa acgcgcaggt actgcgattg ttcggtcgca ttaggccccc
     5881 agacttcgcg caacaggtgg ctatgggtga ccactttgcc ggcattaaga gccagcaccc
     5941 gcaatagatc atactccgtc gccgtaagtt taacctcgcg gccgttcacc gtgacaatgt
     6001 ggccgctgaa gtcgatcgcc aaattgccgg tctggaaata agcatttgcc tcgccctcct
     6061 ggccacgccg ctgtatcgcc cgcaggcgcg ctaggagctc agtggcatcg aacggtttgg
     6121 taacatagtc gtcggctccg gcgtcgaggg ccgcgacttt ttgctccgcc gcctcacgta
     6181 cggaaagaat cagcaccggg acttgcgacc attcgcgcaa tcgcttgaga atttcgatgc
     6241 catccatatc gggcaagccc aggtcgagca gcaccacgtc aggcccgcgt gtggcgattt
     6301 cagcgaggcc ggatttgccg gtttccgctt cgacgacatc gtagccatgc tcttccagga
     6361 ccacccgcaa aagtttgcga atctgtcctt cgtcatcgat gacgatagcg gtcgctttat
     6421 cgttcaatcg gcaagctcca tatttttttc attcaaggct tccgccggca cacggattgt
     6481 gaactcggcg ccgccgcccg ggcggttggc cgcatgcacg ctgccgccat gagcgcggat
     6541 aaagccctcg acgatcgaca gccccaatcc ggtgccccct gacggcgcct tcgcaccacg
     6601 ataaaatttt tcgaacacat gcggcaattc ttcttgagga atccccggac ctctatccgc
     6661 cacaatcagc cgtaattcat tgtcgccgat atcggcgctc acggtgattg tcttgccgga
     6721 ctcgctccaa ttcgccgcat tggcgagaag attcgccagg gattgctcga tcaacaagtg
     6781 gtcaatcttt atcatcggta aattctcttt gatatcgacc tctaatgggt gtctcgaaag
     6841 agcctccgcc gcgagcgcca ctgcgccctc gacaacttcc tcgatatcgc accaatcgag
     6901 cttgggctga accacgcccg tttcgatccg cgtcatctcc agcaagttac cgattacccg
     6961 gttcaaccgc cgtgtggcga atttaatctc gcccaccgtt tcggtctgag ccaggtcgtt
     7021 cttactttgt tgctcaagcg ctgccagacc cgtctgcacc gccgccaacg gtgttttgag
     7081 ttcatgggaa acgctgtcga gaatgctacg ccgtaaacgc tcggaagctt caaggatttc
     7141 agcgcgctgg atcgccgccg cgatgtgatc cttttcgagc aagagaccga tcaagaccgc
     7201 gaaggcttca agcaaatcgc gctccgataa atccaaagcg cggtccatag gtagccgtat
     7261 acagaggacg cccatcgccg ccgtgcgcgc ctgtaacggc aagtataacg cctccgcatc
     7321 gggtagcgtg tcggtgaatc ggccggcggg cgcgcgccgg ctaaatgccc aggcggcaac
     7381 gctctgctcc ttctccgaaa gcgcaaacga acttgccggg ttgacggtat tgtcgagagt
     7441 gtggtccgac tggcgaagca ccagcgcggc tttagcgtcc atgatcgatt cgatatgcgc
     7501 caccgcagcg cgcaaacccg cgtctagctc aggcgcaaat gccgcctggt gcgccagttc
     7561 gtaaagcgca gcggtgcgcc gttcccgttt gcgctcggct atctggctct gatgcaagcg
     7621 gccggtgaga tgccccatcg ccagcgcgac gatgaaaaac atcgcgaaca tcatgacatc
     7681 gtggagcttg ccaatgtaaa aagtaagttg tggggggata aagagaaagt tccacagcaa
     7741 tgcgcttagc gtcgcggcag cgaaaactgg agcgcggccc aggcgcaccc ccaggaccac
     7801 caccaacaag agatacaaca gcgctaccgc aagatagcct gtgatgggtt cgaacccgag
     7861 tgaaatcacg gtcacgccgg caacgcccag agcggcgatt agaaattcat gcaacggtga
     7921 ctgcatggtt cttgcggcac ccgaccgggt tggcgcgaca acgagtcggt cggcttgtac
     7981 gacataaacg tcaatggcgc cgctccgatg aatcaagcgg ttgagcagcc aacggccttt
     8041 caaccaaccc cagccaccgg cgaccgcggg cttaccgaca ataatctggg ttacgttctg
     8101 ttgtcgtgcg atccgcagca acgcttcact gcggtcgtta cctgtagtca ggacgacttc
     8161 gccgcccaat tgccgcacaa gggaaaggct gcgtgtgagc tgatctttat tttcggagcc
     8221 aactgccctg tcggggtcga cataaaccgc cagccaggaa cagccgaact caccggcgac
     8281 gcggcgggtc cagcgaatca agctctccga accggcgtcg ccgccaacgg caaccatcag
     8341 ccgctcactg cttttccaag gcccgccg
//
LOCUS       DASQKS010000282         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1402746, whole genome shotgun sequence.
ACCESSION   DASQKS010000282 DASQKS010000000
VERSION     DASQKS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402746"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VE170_07335"
     CDS             complement(<1..742)
                     /locus_tag="VE170_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HYJ15286.1"
                     /translation="MKVLVLNCGSSTLKFQVMETDGATRPRKLAQGIVERIGGTAAYS
                     FKTANGGSPEGKTLPVADYEAAARLVIDWLRSRSELPAIDAVGHRVVHGADRFADSVL
                     IDDTVIAELEALCEIAPLHNPAALGGIQASRKILGEALPMVAAFDTAFHHMIPEPAAT
                     YAIPYELSQKYKIRRYGFHGLAHQYDVGRYAELIGKPVAQVSAVTLHLGNGCSATAIR
                     DGRSVDTSMGFTPLEGLVMGTRSGDLDPA"
     gene            complement(1109..2797)
                     /gene="glgP"
                     /locus_tag="VE170_07340"
     CDS             complement(1109..2797)
                     /gene="glgP"
                     /locus_tag="VE170_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYJ15287.1"
                     /translation="MNHRAIAYFSMEIGLASDIPTYSGGLGVLAGDTIRAAADLKVPI
                     VAVTLLHRKGYFFQRLDHEGRQIEEPVDWAVDDFLTELPQRTSVTIEGRLVFLRAWRY
                     DVAGPGDFAVPVYFLDADLPENSEWDRTLTHYLYGGDRHYRFCQEIILGIGGVRMLRA
                     MGHREIERFHMNEGHSSLLALELLDEEVARAGKQALTRDDIEWVRQKCVFTTHTPVPA
                     GQDQFPLDLVHRVLGKREVEEMTDVFCCDGVLNMTYLALNLSHYVNGVAKKHGEVSRL
                     MFAGYNIDAITNGIHGATWTAGALAQLYDRYISGWHLDNCSLRYALNIPKQEIWQAHV
                     AAKTALLHHVNQESNAGMDADVFTVGFARRAATYKRTDLLFTDIERLKAIASRRGGLQ
                     IVYAGKAHPQDEAGKEQIRHVFRAREQLRDKIKIAYLENYSMEIGKLMTAGVDLWLNT
                     PLPPMEASGTSGMKAAVNGVPSLSVLDGWWIEGCIEGVTGWSIGAKDGAAGMVADHSR
                     DAASLYDKLEHVVMPQFADNRDDFIEVMRHAIALNGSFFNTHRMLQQYVLKAYF"
     gene            complement(3242..3817)
                     /locus_tag="VE170_07345"
     CDS             complement(3242..3817)
                     /locus_tag="VE170_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ15288.1"
                     /translation="MSTEPVVIEEPRIARLLFSDTRFGWLWLPLRLYLGYMWFEAGWH
                     KLLDPKWMGTGEALLEYWQRGLKMTPKPAISFDWYRGFIEFLVSAEAHTWFSKVIIFG
                     ELAIAAGLILGAFVGVAAFFGGLMNWSFIMAGSASTNGMLFAIATWLVLAWRNAGWIG
                     LDRWLLPALGTPWKPGRRLEGRRIVKVSYED"
     gene            complement(4176..>4428)
                     /locus_tag="VE170_07350"
     CDS             complement(4176..>4428)
                     /locus_tag="VE170_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15289.1"
                     /translation="QIPLLAIHCVVSDDITQRRLAHRESAATDVSDGRWEIYVQQKAA
                     FQPLNDIPEADRVELDTDATPDALAVTCEKFLRTRLARH"
BASE COUNT          996 a         1324 c         1173 g          935 t
ORIGIN      
        1 gcgccggatc gagatcaccc gagcgcgtgc ccatcaccaa accttcgagc ggcgtgaagc
       61 ccatcgatgt gtcaaccgac cggccgtcgc gaatcgccgt cgccgaacag ccgttgccca
      121 agtgcagcgt gacagcgctt acctgtgcaa ccggcttgcc gatcaactca gcgtaacgcc
      181 cgacatcgta ttgatgcgcc aacccgtgaa agccataacg gcggatctta tacttctgtg
      241 acagttcgta aggaatagca taggtcgccg ctggttccgg gatcatgtga tggaaagcgg
      301 tgtcgaacgc cgcgaccatc ggcagggctt cgccgagaat cttccgcgac gcctggatgc
      361 cgccgagcgc cgccggatta tgcaatggcg cgatctcgca gagcgcttcg agctcagcga
      421 tcactgtgtc gtcgatcagc accgaatcag cgaatcgatc cgcgccatgc accacccgat
      481 ggcccaccgc atcgatggcg ggcaattccg accgcgacct cagccaatcg atcaccaagc
      541 gcgccgcagc ttcgtagtcg gcaaccggca acgtcttccc ctccggcgag cccccgtttg
      601 cagttttgaa agaataagca gccgtcccgc cgatgcgttc gacaatgcct tgcgccagtt
      661 tgcgaggccg agtcgctcca tcggtctcca tgacctgaaa cttgagcgtc gaactgccgc
      721 agttgagcac caggactttc atcttccaga ggcttgtttt tttttgtcgg cttggcgccg
      781 agactcgacg ccgcacgcag acgacattca ggttggcact cccaaacgcg gcaacaccca
      841 acgcatgagc acgatgtagg cgacgataaa cccaatgaac cataagccgt cggtcatgtt
      901 gttttactcc gatcatgagt ggcaatccgg ccatcgattg tgggatgcag cctatcgaaa
      961 ccttcgatta ttccactacc gatcaccatg atcaccgccg cgcgctgggc tcgtctcagg
     1021 gcgcacagcc gcaacgccga tagtttactc agcctccccg acttgagctg aggcattaac
     1081 ctcacccagc gcatcgtcct tgtgccgttc aaaagtaagc cttcaacaca tactgctgca
     1141 acatccgatg ggtattgaaa aacgagccgt tgagcgcgat cgcatggcgc attacttcga
     1201 tgaaatcgtc gcgattatca gcaaattgcg gcatgacgac gtgctcgagc ttgtcgtaca
     1261 gcgatgcggc gtcgcggcta tggtcggcaa ccattccggc ggcgccgtct ttcgcgccga
     1321 tcgaccagcc ggtaacgcct tcgatgcagc cttcgatcca ccagccgtcg agcacgctca
     1381 agcttggcac gccattgacc gctgccttca tgccgctcgt acccgatgct tccataggcg
     1441 gcaacggcgt attaagccaa agatcgacgc cggcggtcat cagcttgccg atctccatgc
     1501 tgtagttttc caggtaagcg attttaatct tatcgcgcag ttgctcgcgg gcgcgaaaca
     1561 catggcgaat ctgttctttg cctgcttcgt cctgcggatg ggctttgccg gcatagacta
     1621 tttgcaatcc accgcgccgg ctggcgatcg ccttgagccg ctcaatgtcg gtgaacagca
     1681 aatcggtgcg cttgtaggtg gctgcgcgcc gcgcaaagcc gactgtaaaa acgtcggcgt
     1741 ccatgccggc gttgctttct tgattgacgt gatggagcag tgctgttttt gccgcgacat
     1801 gcgcttgcca aatctcttgc ttgggaatgt tcagggcgta gcgcaagctg caattgtcca
     1861 ggtgccagcc tgaaatatat cggtcatata gttgtgccag cgcgccggcc gtccaggtcg
     1921 cgccgtgaat gccgttagta atggcgtcga tattgtatcc ggcaaacatg agcctcgaca
     1981 cctcgccgtg ttttttcgcc acgccgttga cgtagtggct gagattgagc gcgagatagg
     2041 tcatgttcag cacgccatcg cagcagaaca cgtcggtcat ttcttcgact tctcgcttgc
     2101 cgagcacgcg atgaaccagg tcgagaggaa actgatcctg tcctgccggc accggagtgt
     2161 gcgtggtgaa aacgcatttc tgtcgcaccc actcgatatc gtcgcgagtt agtgcttgct
     2221 tgcccgcacg cgccacttcc tcgtccagga gttcaagggc gagtagactt gagtggcctt
     2281 cattcatgtg aaacctctcg atctctctat gacccatggc gcgcagcatc ctcaccccgc
     2341 cgatgccgag aatgatttct tggcaaaacc ggtaatggcg atcgccgccg tagagatagt
     2401 gtgtcagtgt gcggtcccac tcggaatttt ccggtaaatc ggcgtcgagg aaataaaccg
     2461 gcacggcgaa atcgcccggc ccggcgacgt cgtatctcca agcgcgcaaa aacaccaagc
     2521 ggccttcgat cgtaaccgag gttcgttgcg gcagctccgt gaggaaatca tccaccgccc
     2581 aatccaccgg ctcttcgatc tgccgtcctt cgtgatcaag tcgctggaag aaataaccct
     2641 tgcgatgcaa cagcgtcacc gccacgatcg gcacctttaa atcggccgcg gcgcgaatgg
     2701 tgtcgccggc caacaccccc agcccgccgc tataggtcgg aatgtcgctg gccaagccaa
     2761 tttccatgga aaagtaagcg atggctcggt gattcataat ctattcagca gtcagaaacc
     2821 tggtattaat caaaccaaca gtaaatggct ggctgaaaat tctcaaacgc acaggtatac
     2881 aagtatttaa tcgttgtcag atcttggaga tattgtgcaa gtgcgatgat ctgccgatcc
     2941 tccgcagttt ggccctgcgc acatcagaaa tagcagatat gtgaagaacg gtctgatagt
     3001 caagaaatcg ctttgcgtaa caccaagctg tgagggggca catgacgatt cagcgggcag
     3061 gcaacggcaa cagctacgaa ggattggacc gacatcgact aaccacgcta agccaaaccc
     3121 cgagcgcctt ttcgtcgtcg gcgcggtggg cggatagtgc agacacggtt gccaaagtcg
     3181 agacgtttaa ggatttggct tctcacgaca aggcggaaag caatttcctc ccgcctgtga
     3241 ctcaatcttc gtaagacacc ttaactatac gacgtccttc taacctacgt ccaggtttcc
     3301 aaggagtccc taatgcaggc aggagccagc gatcgagacc gatccatccc gcgttacgcc
     3361 atgccagcac caaccaggtg gcgatggcga aaagcatgcc gttagtcgac gccgatccgg
     3421 ccatgataaa gctccagttc atcaagccgc cgaagaacgc ggcgacgccg acaaacgctc
     3481 caagaatcaa accggcggcg atagcaagct cgccaaaaat tatcaccttg gaaaaccacg
     3541 tgtgggcctc agcgcttacc aagaactcaa tgaaacctcg ataccagtcg aacgatatag
     3601 ccggctttgg tgtcatcttg agaccccgct gccaatactc aagcaaagcc tcgcccgttc
     3661 ccatccattt gggatcgagc aacttatgcc agcccgcctc gaaccacata taaccaagat
     3721 acaatcgcag cggcagccac agccaaccga atcgggtatc actaaacaag agccgcgcga
     3781 tccgcggctc ttcgatcacc actggttccg tgctcatacc tgttcctccc tcgcgtcgat
     3841 tgccgttgta ttttgtcagg cgcaagcgat atgccacctg atttctccaa aaatctcagc
     3901 aaattgtaga tcttgcaatg aaactcttcg cactccccgg aactgaaact gtatgctttc
     3961 gcagggctgc gaattccaag ccgctagcat gcggctcagg tactccccga aaatcggttg
     4021 tatgccccga gctatcgatc tactgacgac aggaccactc cgccgttatg ttactacggc
     4081 gaaccggagc cacgatcaag tgcagcaggt agcaatgaga tcggcagcga gctatggagt
     4141 gtccgcgccg tgacgggaaa tcgtaatggc gattctcaat gacgggcgag ccgggtgcgc
     4201 aggaattttt cgcaggtaac ggcgagtgcg tcgggggttg catcggtgtc aagttctaca
     4261 cggtcagcct ctggtatatc gttcagaggt tggaaggcag ccttttgttg cacgtagatt
     4321 tcccagcggc cgtccgacac gtccgtcgcc gcgctttccc ggtgcgccaa tcgtctttgg
     4381 gtaatatcgt ctgaaaccac gcagtgaatc gccagcaagg gaatctga
//
LOCUS       DASQKS010000283         7589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4766787, whole genome shotgun sequence.
ACCESSION   DASQKS010000283 DASQKS010000000
VERSION     DASQKS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4766787"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VE170_07355"
     CDS             <1..1047
                     /locus_tag="VE170_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15290.1"
                     /translation="VETWQYLDPEFYLLRPIPVVFPEDTCFGTHNAAWVIDYKLRLFA
                     SNPTSMKRAQDKDASIPVQEMRDVDARLACMDALAIDIQVVFPSIWLGCLAENVELEA
                     ALARSYNQFMATQCNRSGGRLWYVAILPWRRPDLAVQEIRRVKALGSAAGIFARGLEW
                     DRPLTHPDHWPIYQEAERQDLPVTVHTGNGSSPAISRMLEGVPRPYYDEFPQIHPLGR
                     GIVSNPYVLYGFTQILSSTLLDDFPKLRVAFLEAGIEWTPRLVKGLGEKRRAKVDGWL
                     AERVFVSCAIDDDLPYVASKLGDDFIVTATDFPHGDAFRQDQLARGLTNRGDLSSKTI
                     EKILNKNPERLYHF"
     gene            1185..2159
                     /locus_tag="VE170_07360"
     CDS             1185..2159
                     /locus_tag="VE170_07360"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HYJ15291.1"
                     /translation="MKRSFLQRSTSSVIQWFNADFHASGELGKVKDVDRINWVRSIPF
                     IIMHLGCLGVFLVGWSWTAVTTAAALYFVRMFAITGIYHRYFSHRTFKTSRPAQFIFA
                     LLGASATQRGPLWWAANHREHHRNSDTPADPHSPVHFSFLRSHMGWFMCNRFYATQYN
                     RIQDFARFPELVWLNRYDKAVPLTLASLLYICGFGLEHFAPSLGVTGLQLLVWGFVIS
                     TTVLFHGTATINSLSHIFGRKRYDTGDESRNNFWLALITLGEGWHNNHHQHMGTVRQG
                     FYWWEIDVTYYILKSLSWLGIIWDLKPVPVQAYDRSEQLPVKEMASAA"
     gene            2435..3442
                     /locus_tag="VE170_07365"
     CDS             2435..3442
                     /locus_tag="VE170_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15292.1"
                     /translation="MRSISAMIFVCFMAASGQSAFSQSAQSIAAAKKEGGRTILYTSM
                     ETFTADAIKAAFEKKTGLQMEYWRGGSTEVLDRVLSEHRVGRPGFDVVAITGDHMRLM
                     ANEGAIGKYDSPSLKAFAKEAIDPVLGARYRNIMYGVIYNKNQLKAGEYPKTLEDIVK
                     PEFRGKLVMPHPVAHTLTTQWLASLDKIMPKQRVERFVRDLAAAKPLFAESIVPAADK
                     VGSGEVAIGITFARFVLTYNKQGANLDYIRDYNMLGDGQYINIAAKAPRPNAGKAFID
                     FFLDEESMKIQADTGEFVNRKGVYPPLQDADKIKFVQMYQFTKEDFETKKKEYQKIFM
                     Q"
     gene            3557..4630
                     /locus_tag="VE170_07370"
     CDS             3557..4630
                     /locus_tag="VE170_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15293.1"
                     /translation="MAEFIIDADGHIMEDHKDIFAHIKGNFGEMNWHSTWPMFDADGW
                     QRGLARKGKREDPDAERWINFQNEHGIDMAVLYPTSALALGMIQLPEWASALAQGYND
                     WLSNRFTTQSPRLKGVALLAPQDPRAAALELRRCVKELGFVAGLLPSATNNRTPLYGS
                     PVFHEIYDEAQRLDVPLTIHGGVSQNLGLDRVASFLEAHMLEHPVGLFLHITNMMFQG
                     VFQEFPRLRVAYLEAGAGWVPFMMDRMEEDYEKFAARLAPQLKNPPSHFFASGNIFVT
                     MEVEERTAPYVVKLLRDDVIMWASDFPHERERDQFGGDLPTLKARKDLTDEFKKKMLH
                     DNPVRFYRLSEGDIAAARKAKVN"
     gene            complement(4807..5649)
                     /locus_tag="VE170_07375"
     CDS             complement(4807..5649)
                     /locus_tag="VE170_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HYJ15294.1"
                     /translation="MPIFMDRHYIEGATHHAIRNAHEKDLDIQDKYSVKFLTYWFDEL
                     RCTAFCLVDAPNQEVIQKAHAEAHGAIPGEIIEVDPAIVEAFLGRVKDPIQTQQASGV
                     ASQPTIDSAFRVIMFTDLKDSTATTTRLGDAKALHFIHINNALTRNALREFGGREVKH
                     LGDGIMATFLSASNAVECAISIQKAFAAHNQQQPDATLHLRLGLSAGEPVADEGDLFG
                     SAVQLAARLCACAEPDQIVVSQLVRDECQADLYPFLDLGEIKLKGFDSAVRAHAVQWR
                     DVQG"
     gene            complement(5793..6266)
                     /locus_tag="VE170_07380"
     CDS             complement(5793..6266)
                     /locus_tag="VE170_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15295.1"
                     /translation="MKITKGILPAAIFAFVSMAVPSVWAQAGAHTMVMPGDLKWVVVP
                     SLPSGAKLAVVEGPLNAAVSFTFRLRFPADDKLPAHWHAAIEHVTVMSGAFNMGIGDK
                     LDAAKAKRLPAGSVAIMQPKTNHFAWTKEETIVQVHGIGPWTLTYVNPADDPRKK"
     gene            6660..7352
                     /locus_tag="VE170_07385"
     CDS             6660..7352
                     /locus_tag="VE170_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYJ15296.1"
                     /translation="MLGIAGLFTLNLVYQVIRKPGELFVAISGSLAKSPATTWQSYGA
                     LFQKYSTSIISPELLAALAQVESDGNPVARTYWRWQWSWNPFEIYRPASSALGMFQLT
                     DGTFAEARKYCIRDHKVVTEDSNSDSSSCWLNGLYTRTLASHAVELTSAYLHQSVINT
                     LAARGRTKASPGQKQKLAAVIHLCGLKTGETFSAGGFRVTPGLFCGTHSLQRYLGRID
                     LMKKRFAQLAGN"
BASE COUNT         1719 a         2185 c         1934 g         1751 t
ORIGIN      
        1 gtcgagacct ggcagtatct cgacccggag ttctatctgc tccggccaat cccggtggtt
       61 ttccccgagg atacttgttt cggcacccac aatgctgcat gggtgatcga ttataaactg
      121 cgtttgttcg cctccaatcc gacatcgatg aaacgagccc aggacaaaga cgcttctatt
      181 cccgtgcaag agatgcgcga tgtcgacgcg cgcctcgcgt gtatggacgc attggccatc
      241 gacatccagg tggtgttccc gtcgatctgg ctcggttgtc tcgccgagaa cgtcgagctc
      301 gaagcagcgc tggcgcgcag ttacaatcaa ttcatggcaa cgcaatgcaa ccggtcaggc
      361 ggccgcctct ggtatgtcgc catcctgccc tggcgccggc ccgatcttgc cgtccaagaa
      421 atccgccgcg tcaaagcgtt gggctcagcc gccggcatct tcgcccgcgg tctcgaatgg
      481 gatcgaccat tgacccaccc ggaccattgg ccgatctacc aagaggccga gcgccaggat
      541 ctgccggtta cagtgcatac cggcaacgga tcgagcccgg ccatcagccg catgctcgaa
      601 ggcgtgccgc ggccttatta tgatgaattt ccgcaaatcc atccgctggg ccggggcatc
      661 gtcagcaatc cttatgtgct ctatggcttc acccaaatct tgagttcgac actgcttgat
      721 gacttcccta aactgcgcgt cgcctttctc gaagccggta tcgaatggac ccctcgtttg
      781 gtcaaaggct tgggcgagaa gcgccgcgcc aaggtcgatg gctggttggc cgagcgggtc
      841 ttcgtatctt gcgccatcga cgacgatcta ccgtatgtcg cgagcaagct cggcgacgac
      901 ttcatcgtca ccgccacaga tttcccccac ggcgacgctt ttcggcaaga tcaactggcc
      961 cggggtttga caaaccgcgg cgatctaagc agcaaaacaa tcgagaaaat cctcaacaaa
     1021 aatcctgagc gcctatatca tttttaaact tttgcccggt aaatcgccag ggaattggtg
     1081 ctctcggagt tacttatcga ctccccgcga ttttgtccgt ttccgtgaaa aaatctttat
     1141 ttttattgaa gtgacccgtt tctcatgata tcgttcttgg attaatgaag cgctcattcc
     1201 ttcaacgctc cacctcctcg gtgatccaat ggtttaacgc cgactttcat gcctctggcg
     1261 agcttggcaa agtcaaggat gtcgacagga tcaactgggt acgtagtatt cctttcatca
     1321 tcatgcatct tggctgtctc ggggtttttt tagtcggctg gagctggacc gcagtaacta
     1381 ccgcggcggc actttacttc gtgcgcatgt tcgcgatcac cgggatctat caccgttatt
     1441 tttctcatcg tacctttaag acttcgcggc cggcgcaatt tatctttgcc ctcttgggcg
     1501 cgtcggcgac ccagcgcgga ccgctctggt gggcggctaa ccatcgcgag catcatcgca
     1561 actccgatac ccctgcagat ccccattcac cggtacattt tagttttttg cgctcccaca
     1621 tgggctggtt catgtgcaac cgtttttacg ccactcaata taatcgcatc caagacttcg
     1681 cccgcttccc cgaactggtt tggctcaatc gttacgacaa agccgtgccg cttactttag
     1741 cgtcgcttct ctatatttgc ggcttcggtc tggagcattt cgcgccgtcc ctcggggtca
     1801 ccggcctgca acttttggtt tggggtttcg tgatcagcac cacggtatta tttcatggca
     1861 ccgcgacgat taattcgctg tcgcatattt tcggtagaaa acgctatgac accggcgacg
     1921 agagccgaaa caacttttgg ctggcattga tcaccctcgg cgaaggctgg cacaacaacc
     1981 atcatcaaca tatgggtacc gtccgtcagg gcttttattg gtgggagatc gacgttacct
     2041 actatattct caagtcttta tcctggctcg gcatcatctg ggatctgaaa ccggtgccgg
     2101 tccaagccta tgatcgcagt gaacaactgc cggtcaaaga aatggcatcg gcggcttagc
     2161 cggtccagtc aagcttccat aaccgattcc tagttttcaa ggctgcgcgc agtcgcctga
     2221 cgattgggta tccctgcctc aatctcgttc cggccaccag tatggcggtg cagtttcgcc
     2281 aagatttgtt ggcgatacgt tcgtagcgca aatctgcttt ttcatcgacg atcgtagatt
     2341 ccaaattgaa ccgcgatcaa gcttgactcc accgcgaccc gtaccatata aacctcactg
     2401 tcgagcaatg attttccttg tgaaaggaat tccgatgcga agcatttcag cgatgatttt
     2461 cgtttgtttc atggctgcta gcgggcaatc ggcctttagc caatcggcgc agtcgatcgc
     2521 cgccgcgaag aaggaaggcg gcaggactat cctttacacg tcgatggaaa ccttcactgc
     2581 cgacgccatc aaagccgcct tcgagaagaa gaccggactg caaatggaat attggcgcgg
     2641 cggatcgacc gaagtcctcg atcgcgtcct gagcgagcat cgggtcggga gaccgggatt
     2701 cgacgtggtc gccatcaccg gggaccacat gcggctgatg gcgaacgaag gcgccattgg
     2761 caaatacgac tcgccttcgc tcaaagcatt cgccaaagaa gccatcgacc cggtcttggg
     2821 cgcccgctat cgcaacatca tgtacggcgt gatctacaat aaaaaccaac tcaaggcggg
     2881 cgagtacccc aagacgctcg aagacatcgt taaaccggag ttccgcggca agctggtcat
     2941 gccccacccc gtcgcccata cgctgaccac ccagtggctc gccagcttgg acaaaatcat
     3001 gcctaagcaa cgcgtcgaga gatttgtccg cgatcttgcc gcggccaaac cgttgtttgc
     3061 cgagtcgatc gtgccggcgg cagacaaggt cggcagcggc gaagttgcca ttggcatcac
     3121 cttcgcgcgc ttcgttttga cttataacaa gcagggcgcc aatctagact acatccgcga
     3181 ctacaacatg ctcggtgacg gccagtatat caacatcgcc gccaaagcgc cgcggcccaa
     3241 cgccggcaaa gcatttatcg atttctttct cgacgaggaa agcatgaaaa ttcaggccga
     3301 caccggcgag ttcgttaacc gcaagggcgt ttatccgcca ctccaggacg ccgacaagat
     3361 caaattcgtg cagatgtacc agttcaccaa agaagacttc gaaaccaaga agaaggaata
     3421 tcagaagatc ttcatgcagt agctgggcat cagatttatt gctcgctccg gacacattag
     3481 caaagatttc tgctctgaca tttcgatcga aagataaatc tgttttcaat cgctaaccaa
     3541 cgcattcgga ggacccatgg cagagttcat catcgacgcc gacggccaca tcatggaaga
     3601 ccacaaagat attttcgccc acatcaaagg caacttcggc gaaatgaact ggcattccac
     3661 ttggccaatg ttcgacgccg acggctggca acgcggcttg gcgcgcaaag gtaagcgtga
     3721 ggatcccgac gccgaacgct ggataaactt tcagaacgag catggcatcg acatggcggt
     3781 gctctatcca acctcagctc tcgccttggg catgattcaa ctaccggagt gggcctcggc
     3841 gctggcgcaa ggctacaatg actggctctc caaccgcttc accacgcagt cgccgcgctt
     3901 gaaaggcgtc gccctactag ccccgcaaga ccccagagcc gccgcactcg aactgcgccg
     3961 ctgcgtcaaa gagttgggct tcgtcgccgg cctgttgccg tctgccacca ataaccgcac
     4021 accgctctac ggctcgccgg tgtttcacga gatttatgac gaagcgcaga gattagacgt
     4081 gccgctgaca attcacggcg gcgtcagcca aaatttgggt ctcgaccggg tcgcgagttt
     4141 tctcgaggcg cacatgctcg aacatcccgt cggactattt ctccatatca ccaacatgat
     4201 gtttcaaggc gtgttccagg aattccccag gctgcgcgtc gcctacttag aagccggcgc
     4261 cggttgggtg ccgttcatga tggatcgcat ggaagaggac tacgaaaagt tcgccgcccg
     4321 cctggcgccg cagctaaaaa atccgccgag ccactttttc gccagtggca acatcttcgt
     4381 caccatggaa gtcgaagaac gcaccgcccc gtacgtcgtg aagctactcc gcgacgacgt
     4441 catcatgtgg gcttccgact tcccccacga acgcgaacgc gaccaattcg gcggcgacct
     4501 gccgacctta aaggcgcgca aagacctaac cgatgaattc aagaaaaaaa tgctgcatga
     4561 caatcccgtg cgattttacc gcttgagcga aggcgacatc gccgcggcga ggaaagcgaa
     4621 agtaaactaa ttctaaaaac tacgctagct gtgtagtata gcatagggaa ttttcgcgcc
     4681 cgttttcctt caatcaatgg ctattggcgg tggactaacg attgcctcgc ttagaaagac
     4741 tcgcggggac tgaacatacg acgagctctt gccacctcgg cgctaaggcg atgttgtcaa
     4801 acgcccctaa ccctgtacgt ctcgccattg gactgcgtgc gcgcgcaccg cagagtcaaa
     4861 gcctttcagt ttaatctctc ccaaatcaag aaaagggtaa aggtctgcct gacattcatc
     4921 tcgaacaagc tgcgaaacta cgatctggtc gggttcagcg catgcgcaca gtcgcgccgc
     4981 aagttgtacc gcgctgccga ataaatcacc ctcgtccgca accggttcac ccgcgctgag
     5041 gcccagtcgc aagtgtaaag tcgcatccgg ttgttgttgg ttgtgcgccg caaatgcctt
     5101 ctggatagag atggcacatt ccacggcgtt actcgctgag agaaaagtag ccatgatccc
     5161 atcgcccaga tgcttgactt cgcgtccgcc gaattcgcgc agggcattgc gcgttaatgc
     5221 attgttgatg tgaatgaaat gtaacgcctt ggcatctccc aagcgggtcg tggtggccgt
     5281 cgaatctttc aagtcggtga acatgataac gcggaaagcc gagtcgatgg tgggttggct
     5341 agcgactccg gatgcctgct gcgtttggat cgggtctttc actcggccca agaaggcttc
     5401 aacgatcgca ggatcaacct cgatgatttc acccgggata gcgccatggg cttcagcgtg
     5461 agccttctga atgacttctt gattcggggc gtccaccaga caaaatgcgg tgcagcgcag
     5521 ttcatcgaac caataggtca ggaatttgac gctatattta tcttgaatgt cgaggtcttt
     5581 ttcatgtgca tttctaatgg catggtgagt cgcaccttcg atgtaatggc gatccatgaa
     5641 aattggcatg ggaataatct ccccttaaca tatgcccgca atagccgaag catataagcc
     5701 gattacctac tgaccaaagc gggctaaccc tagcggtcgc cgcccgggcg cagcttgccg
     5761 gcagtatcta gtggaccggt taccaggcaa gactatttct tcctgggatc gtctgcagga
     5821 ttgacataag tgagggtcca cggcccgatt ccgtgcacct gcacgatggt ctcttcttta
     5881 gtccaggcaa aatggttcgt ctttggctgc atgatggcaa cgcttccggc cggtagccgt
     5941 ttggccttcg ccgcatcaag cttgtcgccg atacccatat tgaacgcgcc ggacatcacc
     6001 gttacatgtt cgattgccgc gtgccagtga gcagggagct tgtcgtcagc aggaaatctc
     6061 agccggaacg taaaagaaac tgccgcattg agcgggcctt caacgacggc aagctttgcg
     6121 ccggacggca gtgacggaac aacaacccat ttaagatcgc ccggcatgac cattgtatga
     6181 gcgccggcct gcgcccagac ggatggaact gccatgctca caaatgcgaa aattgcagcc
     6241 ggtaatatac ccttcgtgat cttcatgtgt tgccccttat aatatcatgg ctgggaacca
     6301 gccttttgca tcccaactta atggatagcc ttttgtttcg caaagtataa gggcaatcaa
     6361 gacgctcgct gactctagcc ttgcgcctga taaattacca cccatgttat gactaatgat
     6421 gtttgcaatt tcttgagaat cgctcttcct ccccgcccag gcgcagccgt agctatcgat
     6481 cgtcagcgct taaaggtgct catccagatt ctaccggcac tggcacactt gatgcctcat
     6541 caccgagagg ttaatcttct aacgtgacat ctactgttta tagcggcgtc cgctattgca
     6601 agcgaaagtc cagaaggttt gccaaacgcc tccggatacc gagcatcgtc tggtggtgcg
     6661 tgctcgggat tgccgggctg tttacgctta acttggtcta tcaggtgatc cgaaaaccag
     6721 gtgagctctt cgtcgcgatt agtggctcct tggcaaaatc tcccgcaacc acatggcaga
     6781 gttacggcgc gctttttcag aaatattcta cgagcattat ctcgccggaa ttgctcgccg
     6841 cgctggccca agtcgagagc gacgggaatc ccgttgcgcg cacctactgg cgctggcaat
     6901 ggtcgtggaa tccctttgaa atctatcgtc cggcctcaag cgcgctcggc atgtttcaac
     6961 tcaccgacgg aacattcgct gaggcgcgaa agtattgtat ccgtgatcat aaagtggtga
     7021 ccgaagattc caatagcgat tcctcctcct gttggttaaa tggcctgtat acacgaacgc
     7081 tggcgagtca cgcagtcgaa ttgacctccg cctatctgca tcagagcgtg atcaataccc
     7141 tggcagcccg cggcagaacc aaagccagcc cgggacaaaa acaaaaactg gctgccgtga
     7201 ttcacctctg cggattgaaa acaggagaga cattttccgc tggcgggttt cgcgtgacac
     7261 caggcttatt ttgcggcacc cacagcttgc agcgctatct cggtcgaatc gacctaatga
     7321 aaaaacgatt cgcgcaattg gcgggcaatt aggaaaccta ttctccaacg cttctactgc
     7381 atatcgaccg attgccggct actttgctta aactctgttg cgcttaggtg tctggtttac
     7441 ttacctttca attcgggcat tacaacgcgt aaagttacac atccacaagc acattgaaat
     7501 ccacgcctac cttcccagct gcttacgtaa catcttgcaa ttacgcggat tccacattgt
     7561 aaattgtctt ctcgtcagcg gtgaggcgg
//
LOCUS       DASQKS010000284         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3511255, whole genome shotgun sequence.
ACCESSION   DASQKS010000284 DASQKS010000000
VERSION     DASQKS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511255"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /gene="sufB"
                     /locus_tag="VE170_07390"
     CDS             complement(<1..1348)
                     /gene="sufB"
                     /locus_tag="VE170_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYJ15297.1"
                     /translation="MASTTVEDLARREYKYGFVSDIAADTMPPGLNEDVVRLISAKKN
                     EPEFMLEWRLKAYRHWAKLAQADAEPKWANIHYPPIDYQTVRYYSAPKSQNDGPKSLE
                     EVDPELLETYAKLGIPLKEQERLAGVAVDAVFDSVSVATTFKGKLAEMGVIFCSFSEA
                     VHSHPELIKKYLGSVVPYSDNFFAALNSAVFSDGSFCYIPKGVKCPMELSTYFRINAA
                     DTGQFERTLIIADEDATVSYLEGCTAPMRDKNQLHAAVVELIAHKDARIKYSTVQNWY
                     PGDKEGKGGIYNFVTKRGKCLGARSKISWTQVETGSAITWKYPSCILQGDDSVGEFYS
                     VALTNNYQQADTGTKMIHIGKNTKSTIISKGISAGHGQNTYRGLVKIMKGASGARNYS
                     QCDSLLLGDKCGAHTFPYLEVLNNASQVEHEASTSKIGEDQLFYCRQRGISNEDAVN"
     gene            complement(1377..1706)
                     /locus_tag="VE170_07395"
     CDS             complement(1377..1706)
                     /locus_tag="VE170_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYJ15298.1"
                     /translation="MAMGVSVTDRAAARIKELAAAENRSGQWLRVKVVGGGCSGLQYK
                     VDFDMPKGTDKIFEKDGAKVLVDMKSLLYLTGTELDYKDELMQSGFVFQNPNVKKACG
                     CGASFAV"
     gene            complement(1750..2964)
                     /locus_tag="VE170_07400"
     CDS             complement(1750..2964)
                     /locus_tag="VE170_07400"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HYJ15299.1"
                     /translation="MSLQTPIYLDNQATTPMDSRVLAAMLPYFTERFGNAASKSHAFG
                     WEADAAVDTAREQVAKLIGAASPREIIFTSGATESDNLAIKGIADAYRDKGNHIVTCV
                     IEHKAVLDSCKVLAKHGFEITYLPVGSDGLVDLHRLRAVITDKTILLSIMAVNNEIGT
                     IQPVKEIGRLAKEKNIVFHTDATQGVGKFPIAVDDMGIDLLSLTAHKMYGPKGVGALY
                     VRSAKPRVKLSSLIDGGGHERGMRSGTLNVPGIVGLGHACELSQKEMSAEAERLIALR
                     ERLTDGILKQLDDVQINGHAVKRLPGNLNLSFAHIEGESLLMGLKDIAVSTGSACTSA
                     SLEPSYVLKAIGLADELAYSAIRFGLGRFTNAEEIDYTVKRVVEEVSRLRQISPIYNS
                     KKGQQQQANAKQ"
     gene            complement(3066..>5105)
                     /gene="uvrA"
                     /locus_tag="VE170_07405"
     CDS             complement(3066..>5105)
                     /gene="uvrA"
                     /locus_tag="VE170_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYJ15300.1"
                     /translation="LAELARQGFNRVMFDGQSFELGGEIPAVLKNARQLDLVVDRLTL
                     RDGIEKRLADSLEVAARAGQGTIKIEILGAGATPPAPLLFSQNFTCIDCGTALGEITP
                     SLFSFNSPEGACPACNGTGIEIKGGGKRVADETDAAPCSHCRGSRLRAASLAVRLAGE
                     SIAGFAAASIRENLSFFDGLELAPEHSAVGQKIITEIVSRLRCLGELGLDYLSLDRPT
                     TTLSGGELQRVRLATQIGSTLAGVLYILDEPSIGLHQKDNERLIRLLRQLREGGNSVI
                     VVEHDPETIGAADHVIDMGPGAGVNGGEVVAHGTPAELQKNHRSLTGKYLARELEILR
                     PPQRRAGSGNFLQLTGVRRRNLKNLTVAFPIGALSCVTGVSGAGKSTLVMEVVHRGVS
                     QRLQKATGKSVVSQQISGWQSFDRVIAIDQSPIGRMARSNPATYMGLHDHLRELFAQV
                     PEARVRGFKAERFSFNAKGGRCETCQGDGVTRVEMYFLPEMFVTCESCKGRRYNRETL
                     DVKYKGLSIADVLDLTVLQAAELLSAVPPIHDRLRMLREVGLSYLTLGQSAVTLSGGE
                     AQRLKLARELARRSTGRSLYLLDEPTTGLHFDDIKQLLDLLNRLTDLGNTMIIVEHNL
                     DMIKCADFVVDLGPGGGTAGGDIVAQGTPEDIAAHPASATGRYLRGLLPPVNKSQ"
BASE COUNT         1094 a         1512 c         1371 g         1128 t
ORIGIN      
        1 ggttcaccgc atcctcattc gaaatgccgc gctggcggca gtagaaaagc tgatcctcgc
       61 cgatcttcga cgtcgatgcc tcatgctcaa cctgggacgc gttgttcaac acttccagat
      121 aaggaaacgt gtgggcgccg cacttatcac ccaacaatag cgaatcgcac tgtgaatagt
      181 tgcgcgcgcc gctggcgcct ttcatgatct tcaccaaacc acgataggtg ttctggccat
      241 ggccggcaga gatgcctttg gagataatcg tgcttttggt gttcttgccg atgtggatca
      301 tcttggtgcc ggtgtcggct tgctggtaat tattggtcag cgccactgaa taaaattcgc
      361 cgaccgaatc gtccccctgc aaaatgcagc ttggatactt ccaagtaatc gccgacccgg
      421 tttccacctg agtccaggat atttttgaac gcgcgcccaa gcacttgccg cgtttggtga
      481 cgaagttgta gatgccgcct ttgccttctt tgtcaccggg ataccagttt tgcactgtcg
      541 agtatttgat ccgcgcgtct ttatgcgcga ttaactcgac caccgcggcg tgcagctgat
      601 tcttgtcgcg catcggcgcg gtacagcctt cgagataact cacggtcgcg tcctcgtcgg
      661 cgatgatcaa tgtgcgctcg aactggccgg tgtccgccgc attgatgcga aagtaagtcg
      721 atagctccat cgggcacttg acgcccttcg gaatgtaaca gaacgaaccg tcgctgaaga
      781 ccgccgagtt gagcgcggcg aagaaattat cgctgtaggg caccaccgag cccaaatatt
      841 ttttaatcag ctcggggtgg ctatgtaccg cctcggaaaa cgagcaaaaa atcacaccca
      901 tctcggcgag cttgcctttg aaggtcgtcg ctaccgaaac actatcgaac acggcatcga
      961 cggcgacgcc cgcgagccgc tcttgttcct tgagcggaat accgagcttc gcataggtct
     1021 cgagcagttc ggggtcgacc tcttccaggc tcttgggacc atcgttctgc gacttgggcg
     1081 ccgaatagta acgcacggtc tgatagtcga tgggcggata atgaatattc gcccatttcg
     1141 gctcggcgtc ggcctgtgcc agcttggccc aatgacgata ggctttcaga cgccattcga
     1201 gcatgaattc cggttcgttt ttcttcgccg aaatgagccg aaccacgtcc tcgttcaagc
     1261 ccggcggcat ggtatcggcc gcgatatccg agacaaagcc gtatttgtat tcgcgccggg
     1321 cgagatcttc gacagttgtg gacgccataa tcttaatcct ttatttattt actctttcaa
     1381 acggcaaacg acgcgccgca accgcaagcc tttttaacgt tggggttttg gaagacgaag
     1441 cccgactgca tcaactcgtc cttgtagtcg agttccgtgc cggtcaaata cagcaggctc
     1501 ttcatatcga ctaaaacctt ggcgccgtct ttctcgaaaa tcttatcggt gcctttgggc
     1561 atatcgaagt cgactttgta ttgcagaccc gagcaaccgc cgccgacgac tttaactcga
     1621 agccactgac cgctacggtt ctcggcggct gccaactcct tgattcttgc cgcagcccta
     1681 tcagtcacac tcacacccat tgccatagtt tttctcctgt caaaaacgcc gacgacttaa
     1741 tagaagccgc tattgcttgg cgttcgcctg ttgctgctgg cctttcttag agttgtaaat
     1801 cggcgatatc tgtcgcaacc gggacacttc ctctaccacc cgtttaacgg tgtagtcgat
     1861 ctcttctgcg ttggtaaaac ggcccaagcc gaaacgaatg gcgctgtagg ccagttcatc
     1921 ggcaaggccg atcgctttca gcacatagga cggttccaaa ctcgccgaag tacaagccga
     1981 gccggtggat acggcgatgt cctttagccc catcaaaagc gattcgccct cgatgtgagc
     2041 gaagctcaaa ttcaagttgc cgggcaaacg cttgacggca tgaccgttga tctgcacatc
     2101 gtcgagctgt ttaagaatgc catccgtcag acgctcgcgc agggcgatca aacgctcggc
     2161 ttccgcgctc atttcttttt gcgacagctc gcaggcatgg ccaagcccga caatgccagg
     2221 aacatttaaa gtccccgacc gcatgccgcg ctcatggccg ccgccatcga tgagtgaact
     2281 caatttcacc cgcggcttgg ccgagcgcac gtaaagcgcg ccgacgcctt taggtccgta
     2341 catcttatgt gccgtcaatg acaacagatc gatacccatg tcgtcgaccg caatcggaaa
     2401 cttgccgact ccttgcgtcg cgtcggtatg aaacacaata tttttctctt tagccaagcg
     2461 gccgatctct ttgaccggct ggatcgtgcc aatctcatta ttgaccgcca taatcgacag
     2521 caaaatcgtc ttgtccgtga tgaccgcgcg taagcggtgc aggtcgacaa gcccatctga
     2581 cccgaccggc agataggtta tctcgaaacc atgtttggcc aggaccttac agctatccaa
     2641 caccgcttta tgttcgatga cgcaggtgac gatatggttg cctttgtccc gataggcatc
     2701 ggcaatacct ttgatcgcca aattgtccga ctcggtggcg ccgctggtga agataatctc
     2761 gcgcggcgag gccgctccga ttaactttgc cacctgttcc cgcgcggtat cgaccgcggc
     2821 atcagcttcc caaccgaaag cgtggctctt gctggcggca ttgccaaaac gttcggtaaa
     2881 gtacggcaac atcgccgcca gtacccgtga atccatcggc gtggtggcct gattatcgag
     2941 atatatgggt gtttgtagac tcatcgtagc gcttttcaaa ttgcagacct tattagtctg
     3001 atatcaaggt aaccaagact gggatttgag tcaatagcca agaggcactt aactaacttg
     3061 gcgaattatt gagacttatt gactggaggc agcagcccgc gcagataacg cccggtcgcc
     3121 gaagccgggt gggcggcaat atcctctggc gtgccttggg cgacgatgtc tcctccagcg
     3181 gtgccaccac cgggacctag atccacgaca aagtcagcgc acttaatcat atcgagatta
     3241 tgctcgacga taatcatggt gttgccgaga tcggttagac gattgagcag atcgagaagt
     3301 tgcttgatat cgtcgaaatg aagtccagta gtcggctcat cgaggagata gagcgagcgc
     3361 ccggtcgagc gacgcgccaa ttcgcgggcc aacttgagcc gctgcgcttc gccgccagag
     3421 agagttacgg cggattggcc aagggtcaaa tagctcaggc caacctcacg caacatgcgt
     3481 aaacggtcat gaatcggcgg caccgcgctc agcagttctg ccgcttgaag gaccgtcagg
     3541 tctaagacat cggcgatgct caaccctttg tatttgacgt ccaaggtctc gcggttgtaa
     3601 cgccgccctt tacaggactc gcaggtgacg aacatctcgg ggagaaaata catctcgacc
     3661 cgagtgacac cgtcgccctg gcaggtttca cagcggccgc ccttggcgtt gaaggaaaac
     3721 cgttcggctt tgaaaccgcg cacccgcgct tcgggaacct gcgcgaacag ctcgcgcaaa
     3781 tgatcgtgca gtcccatgta tgtcgcgggg ttcgagcgcg ccatacggcc gatcggcgat
     3841 tgatcgatgg cgatcacccg gtcgaagctt tgccaaccgg aaatttgctg cgacacgacg
     3901 ctcttgccag tggctttctg caaccgctgg ctgacgccgc ggtgaacgac ctccatcacc
     3961 agcgtgctct tgcccgcccc cgagaccccg gtaacacagc tcaacgcgcc gatgggaaaa
     4021 gccaccgtga ggttcttcaa gttgcgtcgg cgcacaccgg taagctgcaa aaaattgccg
     4081 ctgccggcac gccgctgcgg cggccgaaga atctccagct cgcgcgcgag atattttccg
     4141 gtaagtgagc gatgattttt ttgtaattcg gcaggcgtgc cgtgcgccac cacttcgccg
     4201 ccgttgacac ctgcgcccgg tcccatgtcg atgacatgat cggcggcgcc gatggtctcg
     4261 ggatcatgct cgacgacgat caccgagtta ccgccctcac gcagctggcg caacaggcga
     4321 atcaatcgct cgttatcttt ctgatgcaag ccgatgctcg gctcgtcgag aatgtagagc
     4381 acccccgcca gggtcgaacc gatctgggta gccagccgca cccgttgaag ctcgccacca
     4441 gacaacgtcg tcgtcggccg gtccagactc aaatagtcga gacccagctc gcccaagcaa
     4501 cgcaaccggc tgacgatctc ggtgataatt ttttggccga ctgcgctgtg ttctggtgcc
     4561 agttcgagcc cgtcgaaaaa tgacagattc tcacgaattg aagccgcggc aaagccggca
     4621 atactctccc cggcgaggcg cacggccagg ctcgcggcgc gcaagcgact gccgcggcaa
     4681 tgggaacagg gcgcggcatc ggtctcatcg gcgacgcgct tgccgccgcc cttgatctct
     4741 atgccagtac cattgcacgc cgggcacgcc ccttccgggc tgttgaatga aaacagactc
     4801 ggggtaattt ctcccagcgc cgtaccacaa tcgatgcaag tgaaattctg actgaaaagt
     4861 aatggcgccg gcggcgttgc accggcgccg aggatctcga tcttgattgt cccctgcccg
     4921 gcccgcgcgg caacttccag agaatcggcc aagcgcttct caatgccgtc gcgcagcgtc
     4981 agccggtcga cgacgagatc gagttgtctc gcgttcttta aaacagcggg gatctcgccg
     5041 ccaagttcga agctttggcc gtcgaacatg acacggttaa atccctggcg cgcgagttct
     5101 gctaa
//
LOCUS       DASQKS010000285         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3090798, whole genome shotgun sequence.
ACCESSION   DASQKS010000285 DASQKS010000000
VERSION     DASQKS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090798"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VE170_07410"
     CDS             <1..1274
                     /locus_tag="VE170_07410"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15301.1"
                     /translation="VFGGGIALYFWLAVILPLFLLIPAAIGALVTHLLVYAMPAKRIR
                     DLLFFIGLFGFLIMYFLFRFSQPERLVQPESFGNFMQFMSAMETPSSPYLPSSWLAEI
                     LAGTLFKRDTDQGFFFALLTSYAMFLPVAASWCSSAVYLDGWSKAQESRQGRRKHAWI
                     DRMLDAITRPFPAITRAIMLKDIKTFLRDTTQWSQLFLLAALIVVYLYNFKVLPLDRS
                     PMPAGTIRTIVSFANLGLAGFVLSAIAIRFAFPAVSLEGKAFWLLQTAPISLRALLWS
                     KFWMNFIPLLCLGEVLIFCSNLLLKVPAWMMALSLVTMFLMTFGIAAIGVGLGALYPN
                     FNYENAAEIPTSFGGAVCMIFSIGFIAIAVMIIAWPIYTLAMRAFHHGRLFLPEYSVI
                     APSLLIVLALTVVTVIVALRVGIKHLEQLKE"
     gene            1394..2065
                     /locus_tag="VE170_07415"
     CDS             1394..2065
                     /locus_tag="VE170_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYJ15302.1"
                     /translation="MNLIHTIYQPPGNSPFPTVLTLHGRGANAFDLLGLSPHLCGGNL
                     LVICPQAPMETPIGPEQVGYAWYAASEGGTPDTDGMLASKKALELFLDDCLKQYPIDP
                     KRLAVLGFSQGGVMAYSLALTNPGRFAGLAALSTWLPRELVPRLKISDAVRSLPTLVQ
                     HGVQDGQIDVDRARDSVARLRELKVPLTFKEYEMGHEIRPRELYDLSAWLEENVRASG
                     RSPSL"
     gene            complement(2076..2960)
                     /gene="htpX"
                     /locus_tag="VE170_07420"
     CDS             complement(2076..2960)
                     /gene="htpX"
                     /locus_tag="VE170_07420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HYJ15303.1"
                     /translation="MNGFRTTILLAVLTALVVWIGQMVGGPNGAVIALVLAGAMNFFS
                     YWFSDKIVLKMYGGQQITGNDDPELYSLVQELAAKAGLPMPKVYVIPEETPNAFATGR
                     NPEHAAVAVTHGIRRILNKRELGGVLAHELSHVKNRDILISSIAATLAGAISYMAQMA
                     QFAAIFGGGRSRDDDQGGGGGIFGLLFMMIVAPIAAMLIQMAVSRSREYMADATGAKI
                     SGDPLALANALRKLHMGAQNIPLQVSPATQNATAHMFIVSPLSGGGLASLFSTHPAME
                     ERIARLEAMAKDMSYMRA"
     gene            3131..>3771
                     /locus_tag="VE170_07425"
     CDS             3131..>3771
                     /locus_tag="VE170_07425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYJ15304.1"
                     /translation="MQIGVVFPQTEIGADPSYLKDYAQTAEDLGFSHILAYDHVLGAN
                     PASRPNWQAPYSHLDMFHEPLVLFGYLAGVTKTIELCTGIVILPQRQTVLVAKQAAAV
                     DVLSGGRLRFGVGIGWNPVEYEALGEDFKNRGRRCEEQVEVLRQLWTNPTVTFAGHWH
                     TITDAGINPLPVQRPIPVWFGGSDERALQRLARLGDGWFPLVGPDEKCRALID"
BASE COUNT          770 a         1158 c         1004 g          839 t
ORIGIN      
        1 cggtgttcgg cggcggcatc gcactctatt tttggcttgc cgtgatcctg ccgctgtttt
       61 tgctcattcc ggcggccatc ggcgcgctgg tcacacatct gttggtctat gccatgccgg
      121 ccaaacgcat ccgcgacctg ctctttttca tcggcctgtt cggttttcta atcatgtatt
      181 ttctgtttcg cttctcgcaa cccgagcgct tggtgcaacc ggagtccttc ggcaatttca
      241 tgcagttcat gagcgccatg gagacgccgt catcgcccta cctgccgagc agctggttgg
      301 cggaaatcct cgccggcact ttgttcaagc gcgacaccga ccagggattc ttcttcgcgc
      361 tgctcacgag ctacgctatg tttctgccgg tggcggcgag ttggtgctcg agcgcagtct
      421 atctcgacgg ctggtcgaaa gcccaagagt cgcgccaggg ccggcgcaag cacgcctgga
      481 tcgaccgaat gctcgatgct attaccaggc catttccggc gatcacccgc gcgataatgc
      541 tcaaagacat caagaccttc ctgcgcgaca ccacgcaatg gtcgcagctc tttttgttgg
      601 ctgctctgat cgtcgtctat ctttataatt tcaaagtcct gcccctcgac cgctcgccga
      661 tgccggcggg caccatccgc accatcgtct ccttcgccaa cctgggctta gctggattcg
      721 tcctcagcgc catcgccatt cgcttcgcct ttccggcggt gagtttggaa ggcaaagcgt
      781 tctggctgct ccaaaccgcg ccgatctccc tgcgggcatt gttgtggagc aagttctgga
      841 tgaacttcat accgctctta tgcctcggcg aagtgctgat attttgcagt aatctcctgc
      901 tcaaggtgcc ggcctggatg atggcgctgt cgctcgtcac gatgtttttg atgaccttcg
      961 gcatcgccgc catcggcgtc ggtctgggcg cgctctatcc caacttcaat tatgaaaacg
     1021 ccgccgaaat acccaccagt ttcggcggcg cggtatgcat gatcttcagc atcggcttca
     1081 tcgctatcgc cgtgatgatc attgcctggc cgatctatac cctggccatg cgcgcctttc
     1141 accacgggcg cttatttcta ccggaatatt ccgtgattgc gccgtcactc ttgatcgtgc
     1201 tagcacttac cgtcgtaacc gtcatcgtcg cattgcgcgt cggcatcaag cacttggaac
     1261 aactaaagga ataacccgct cataattctt cgacagaccg cccgcttacg gcacaaggca
     1321 aaagggaaac caggcaaacc aaattagcga taccttgcac aagcggtcga gaaaaaactc
     1381 tcaggagaat ttagtgaacc tgatccacac gatctaccag ccgcccggca acagtccgtt
     1441 tccaaccgtt ctcaccttgc atggccgtgg tgccaacgcc ttcgatctcc tcggcctttc
     1501 gccccatcta tgcggtggca acctgctagt tatctgcccg caggcgccaa tggagacgcc
     1561 catcggaccc gaacaagtcg gttacgcttg gtatgccgcc agcgaaggcg gcacaccgga
     1621 caccgacggc atgttggcgt cgaagaaagc cttggaactg tttctcgacg actgcctcaa
     1681 acaataccca atcgacccga agcgactagc cgtcctcgga ttcagccaag gcggtgtaat
     1741 ggcttacagc ctggcattga ccaatcctgg acggttcgcc ggcctcgccg cgctcagcac
     1801 gtggctgcca cgggaactgg tgccgcggtt gaaaattagc gacgcagtgc gctcgctacc
     1861 aacgttggtg caacatggcg tccaagatgg gcagattgat gtcgaccgcg cccgcgactc
     1921 ggtggcgcgc ctacgcgaac tgaaagtgcc gctgacgttt aaagaatacg agatgggcca
     1981 cgagatcagg cccagggaac tttacgatct atccgcttgg ttggaagaaa acgtgagggc
     2041 gagcggccgc tcgccctcac tgtgaatcgc ttaaattacg ccctcatata actcatatcc
     2101 ttcgccatcg cttcgagacg agcgatacgc tcttccattg ccgggtgagt gctgaacagt
     2161 gacgcgagcc cgccaccgct gagcgggctg acgatgaaca tatgcgctgt ggcattctgc
     2221 gtcgccgggc tcacctgcag cggaatgttc tgggcgccca tatgcagttt gcgcaacgcg
     2281 ttggccaagg ccaacgggtc gccggaaatc ttcgctccag tggcgtcggc catgtactcg
     2341 cgcgagcgcg acaccgccat ctgaatcaac atcgcggcga ttggcgcgac aatcatcatg
     2401 aacaagagtc caaagatgcc gccaccgccg ccttggtcat cgtctcggct ccgtccgccg
     2461 ccgaatattg cggcaaactg cgccatttgc gccatataac taatcgcgcc agcgagggtc
     2521 gcggcgatgc tgctaatcaa gatatcgcga tttttcacat gggagagctc gtgagcgagc
     2581 acgccgccga gctcccgctt atttagaatc cggcgaatgc cgtgggtcac cgccaccgcg
     2641 gcatgttcag gattgcgccc tgtggcaaac gcattgggcg tctcctcggg aatcacatac
     2701 accttgggca tgggcaaacc ggctttggcc gccaactctt gcaccaaact atataactcc
     2761 ggatcgtcat tgcccgtgat ctgttgcccg ccatacatct tgagcactat cttgtcgctg
     2821 aaccagtagc taaaaaagtt catggcgccg gctaagacca acgctatcac cgcgccattg
     2881 gggccgccga ccatctggcc gatccaaacc acgagcgcgg tcaaaactgc caggagaatt
     2941 gtggttcgaa aaccgttcat aaatttcctc cctttctgtg aggtatccta cggaatgaat
     3001 gggttaagtc aaggtacaat tttagtccta gagaatctgc gcattgccgg cgcttatttc
     3061 aagtccccct atttctaaac cgagcggatt tgccatacta tccagtagtt accgagagaa
     3121 ggggttgatc atgcaaattg gagtcgtctt tccgcaaacc gaaatcggcg ctgatccgag
     3181 ttatctcaaa gactacgccc aaaccgccga agatctcggc ttttcccata ttctagccta
     3241 cgaccacgtg ctcggcgcca atccggcaag ccggcccaat tggcaagcgc cctacagcca
     3301 tttagacatg ttccacgagc cgttggtgct gttcgggtac ctcgctggcg ttaccaagac
     3361 aatcgagttg tgcaccggca tcgttattct gccgcagcgt cagaccgtct tggtcgccaa
     3421 gcaagccgct gcagtagatg tattgagcgg cggccgtttg cgtttcggcg tcggcattgg
     3481 ctggaaccca gtggaatatg aagccctcgg cgaagatttc aagaatcgcg gtcgccgctg
     3541 cgaagagcaa gtcgaagtcc tgcgtcagct ctggaccaat ccgaccgtga cgttcgccgg
     3601 gcactggcat acaatcactg acgccggcat caaccccctg ccggtgcaac ggccgatccc
     3661 ggtctggttc ggtggcagcg atgaacgcgc cttacaacga ctggcgcgac tcggcgacgg
     3721 ctggtttccg ctcgtcgggc cggatgaaaa atgccgtgcg ctgatcgata a
//
LOCUS       DASQKS010000286         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5180441, whole genome shotgun sequence.
ACCESSION   DASQKS010000286 DASQKS010000000
VERSION     DASQKS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5180441"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1351)
                     /locus_tag="VE170_07430"
     CDS             complement(248..1351)
                     /locus_tag="VE170_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HYJ15305.1"
                     /translation="MAEMNTQPADLKPDRLDTYKVWQAEQRIPCIRGFFVKDVNQVPV
                     EPWDLKGVPCSFVVLDGTGGTNDGYVCEIPAGGKTKPQKHMYEEMVYVSKGYGATTVW
                     QRDGKKKHTFEWGPGSMFAIPINADYQHFNASGSETARYFAVTNSCFMMNLFHNSDYI
                     FNSDYVFTDRFDPHTEGYFNGEGTITGRFFMTTNFVPDTHTIKLTDYSERGKGSTNMK
                     FDLARNTMGAHISEFPVGTYKKGHRHGPGAHVIILSGQGYSILWKDGEATERVDWGPG
                     SVVVPPDQWFHQHFNSGAAPARYLALRWNNWRYRFLRSTDGEGGTYTSLKLGGNQIEF
                     EDEDPQVHKDFELAMKAVGAKCNMPYHPGCTQK"
     gene            complement(1428..2594)
                     /locus_tag="VE170_07435"
     CDS             complement(1428..2594)
                     /locus_tag="VE170_07435"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15306.1"
                     /translation="MLTKEENETLTHVGRGTPGGEYLRRYWMPVACAGELTEEKPIKA
                     FRLMGENLVVYRDQSGRYGVVAEQCPHRKASLAFGRVDADGIRCPYHGWKFDGAGKCL
                     EQPAEPVDGGFKDKIKHTAYPAERLGGLIWTYLGPEPRPLLPRWDVLVWEDGYRWIEK
                     HEVYNCNWLQPTENSVDPSHLFWLHGDTAHLAPSVDHYEEDHSFTPFEYGIMKRRVTP
                     GKKPGDAPVVDQHPLLFPSTLRHVFRERLFEGRVRHNLQIRVPVDDAHTQVFVVYFSP
                     NDSDRSPADGDTPWEFFRIRDDNGDYRLDHVLAQDAMAWETQGAPTDRTQEHLGVGDE
                     GIILLRKILREQIDTVRNGGEPLGVVRDAQKNRLIEFEVINQRIGIVAREKKVA"
     gene            complement(2728..>3531)
                     /locus_tag="VE170_07440"
     CDS             complement(2728..>3531)
                     /locus_tag="VE170_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15307.1"
                     /translation="NTLYKGKALDPLKPLLILPEVTDLSKWYGKEHRYADPEGKYIFA
                     FIGSPSNAQLAYNTNLVNPKEFKSYADVINPKWKGKIVSLDPRDTGLGATMQFYYYSP
                     EIGPEFMKKFFGGMEITYAKNFQQMTNWLAQGKYAICMGCKDSARAKKQGLPVDDFDT
                     NHWKEGSSFSAGGGSMSYMNQAPHPNAAKVFLNWFLSRRGQIALQKLGDVDDPANSRR
                     TDIPKDDIPPENRLQPGLKYFDVVKPEYGDMKPIFDLAKDIMAATDKGK"
BASE COUNT          693 a         1027 c         1005 g          806 t
ORIGIN      
        1 agaaacttga gctggcccaa aaagtctcag acagatcggc ataggtcggc gccagctcga
       61 cccccttaag gctggatgct tcaatcaatc aaatggttga agcgctgatt gaatgccgaa
      121 acgaaactct atctgagtcg cggcatgagg cttcggttca aaattcaagg tcggagaggg
      181 ttccgagttc cgaaccttga acttttgaac gcttgaacct ttgaacagaa gtagtcgcgc
      241 ggcgcgacta cttctgtgtg caccccggat gatacggcat gttgcacttc gcgccgacgg
      301 ccttcatggc caactcgaag tccttgtgga cctgcggatc ctcgtcctcg aactcgatct
      361 ggttgccgcc gagtttcaag ctggtatagg ttccgccttc accgtcggtt gagcgcagga
      421 agcggtagcg ccagttgttc caacgcaagg ctaaatagcg tgccggggcc gctccggaat
      481 taaaatgctg gtggaaccat tgatccggcg gcacgacgac actgcccggc ccccaatcga
      541 cgcgctcggt ggcctcgccg tctttccaca agatcgaata gccctggccg gagagaataa
      601 tcacatgggc gccggggccg tggcggtggc ctttcttgta ggtgccgacg ggaaactcgg
      661 agatatgcgc gcccatggta ttgcgcgcaa gatcgaactt catgttggtg ctgcccttgc
      721 cccgctcgct gtagtccgtc agcttgatcg tgtgagtgtc aggaacaaaa ttggtcgtca
      781 tgaagaaccg cccggtgatc gtcccttcgc cattgaagta accttcggta tgcggatcga
      841 agcggtcggt gaaaacgtaa tcgctgttaa aaatgtaatc gctgttgtga aacaggttca
      901 tcatgaagca gctgttggtg accgcgaagt aacgcgcggt ttcactgccg ctggcgttga
      961 agtgctgata atcggcgttg atcgggatcg cgaacatgct gcccggtccc cactcgaagg
     1021 tatgcttctt cttgccgtcg cgctgccaca ccgtggtcgc gccgtagcct ttggagacgt
     1081 agaccatttc ctcgtacata tgcttttgcg gcttggtctt gccgccggcg ggaatctcac
     1141 agacgtagcc gtcgttagtc ccgccggtgc cgtcgagcac gacaaaggaa caaggcacgc
     1201 cttttaaatc ccaaggctcg acgggaactt ggttgacgtc cttgacgaag aaaccgcgaa
     1261 tacacggaat tcgttgctcc gcctgccaaa ccttataagt gtcgagccgg tccggcttga
     1321 gatcggcggg ctgcgtgttc atttcggcca taagaacgtc ctcctgagat tcctagatgc
     1381 gttcgatttg tgcgatacgg tccgatcgtc atacgaaagt ggagtgttca cgccaccttc
     1441 ttctccctag cgacaatccc gatgcgttga ttaatcactt cgaactcgat caaccgattc
     1501 ttttgcgcat cgcgcaccac gcccagcggc tcgccgccgt tgcgcacggt gtcgatttgt
     1561 tcgcgcagga tttttctgag cagaatgatg ccctcgtcgc cgacgcccaa atgctcctgg
     1621 gtgcgatcgg tcggcgcgcc ttgagtttcc caggccatgg cgtcctgcgc cagcacatga
     1681 tcgagccggt aatcgccgtt gtcgtcgcga atccgaaaaa attcccacgg cgtgtcgccg
     1741 tccgccggcg aacggtcgct gtcgttgggc gaaaaataca ctacgaatac ctgggtgtgg
     1801 gcatcgtcca ccggcacgcg gatctgcaga ttatgacgca cccggccttc gaacagccgc
     1861 tcccggaaca catggcgcaa ggtgctggga aacaacagcg gatgctggtc gaccaccggc
     1921 gcatcgccgg gctttttgcc gggggtgacg cgccgtttca tgatgccgta ctcgaacggc
     1981 gtgaagctat gatcctcttc atagtgatcc acgctcgggg ctaaatgcgc ggtgtcgccg
     2041 tgcagccaaa ataggtgcga cgggtccacc gaattttccg tcggctgcaa ccagttgcag
     2101 ttataaacct cgtgcttctc gatccagcgg taaccgtcct cccacaccag gacatcccag
     2161 cgcggcagca gcggccgcgg ctccggtccc aggtaggtcc agatcaatcc gcccaagcgc
     2221 tcggctgggt acgccgtatg tttgatcttg tccttgaagc cgccgtcaac cggttccgcc
     2281 ggctgttcga gacattttcc cgcaccgtcg aatttccaac cgtgatacgg gcagcggatg
     2341 ccgtcggcat cgacgcggcc gaaagctaac gaagcttttc ggtgcgggca ctgttcggcg
     2401 accaccccgt aacggccgct ttggtcgcga tagaccacca gattttcacc catcaggcgg
     2461 aacgctttga tcggcttctc ctcggtcagc tccccggcgc acgccaccgg catccagtag
     2521 cgccgcaaat actcgccgcc cggcgtgccg cggccgacgt gggtcagagt ctcgttttct
     2581 tctttggtga gcattggctc tcctcgattc gtactgcgtt aaatcgccta tgagtcaaat
     2641 tgccgaagcg agtgcctgcg ccgtgtcaat gcctccggcc tctcggagcg ggtagatatg
     2701 cgggctgatc tgcgcggctc gttcaggcta cttgcccttg tccgtcgcgg ccatgatgtc
     2761 tttggccagg tcgaagatcg gtttcatatc gccatactcc ggttttacca catcgaagta
     2821 cttcaacccc ggctgcaacc ggttctccgg cggaatgtcg tctttgggga tatcggtgcg
     2881 ccgcgagttg gccgggtcgt cgacatcgcc aagcttctgc aaggcgatct gtccccggcg
     2941 cgagagaaac cagttgagaa aaactttcgc ggcattggga tgcggcgctt gattcatata
     3001 gctcatcgag ccaccgccgg cggagaagct cgaaccctct ttccaatgat tggtatcgaa
     3061 atcgtccacc ggcaagccct gctttttcgc ccgcgccgaa tccttacagc ccatgcagat
     3121 ggcgtacttg ccttgggcca gccagttggt catttgctgg aaattcttcg cgtacgtaat
     3181 ctccatgccg ccgaaaaatt ttttcatgaa ctcgggaccg atttccgggc tgtaatagta
     3241 gaactgcatg gtcgcaccca aaccggtatc gcgcgggtca agggatacga tcttgccctt
     3301 ccacttggga ttgatcacgt cggcgtagga tttgaactcc ttgggattga ccagattagt
     3361 attgtaggcc agctgggcat tgctcggcga gccgatgaag gcaaagatat atttaccctc
     3421 aggatcggcg tagcgatgtt ccttgccgta ccatttcgag aggtcggtaa cttcggggag
     3481 aatcagcagc ggcttgagcg gatcgagcgc tttgcctttg tagagcgtgt t
//
LOCUS       DASQKS010000287         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_573349, whole genome shotgun sequence.
ACCESSION   DASQKS010000287 DASQKS010000000
VERSION     DASQKS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573349"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1252)
                     /locus_tag="VE170_07445"
     CDS             complement(278..1252)
                     /locus_tag="VE170_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYJ15308.1"
                     /translation="MPQEMTFGEALLAAIDEVAEQDKRIVLFNPSFAGEAGHEKLAAF
                     RKKYAARIKFPPIAEIGFCGIAIGAAMAGLRPVVDISTATFSYEAIPQIVNEAAIAYS
                     NSGGQTNVPVVFYLKFGIRGGGGVQHSGSPQSWYWNTPGLQIVMPSTPADAKGLMRTV
                     LTRSEDPTLFFGHDRLLDDVGEVPDGAYEIPLGKADIKRAGGDLTIIATSIQVPRALA
                     AAEMLAKEGISAEVIDPRTLQPLDKPALLASVKKTGRVLITDESHDNCGVAAGLAAII
                     ADEGFTSLRAPIKRVSIPPVPVPYSVPLEDYVTPMTQRIVETARALCK"
     gene            complement(1255..2271)
                     /locus_tag="VE170_07450"
     CDS             complement(1255..2271)
                     /locus_tag="VE170_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYJ15309.1"
                     /translation="MLPDISRDKQLAILRRMLMIRRAEEHCIRFNDEHTGLIRGHFHV
                     YIGQEATGVAVCDWLNADDYVFTTHRNHGHVIAKGGDPKKVLAEIIGRATGYCQGRAG
                     TFHVAAPEVGVIHTSAIVGGCLALAGGTAYAAQVKDSKAITVVFFGDGAMEEGVFYEA
                     LNIAKLWQLPMIFWMENNYRRSGRGAGHAATELTNVPKALNVETTVIDGIDVGTVHRT
                     AKDIVGRVRRGEGPLFVEARTHLWPGNAFNMPKLVGGVTDISWAWDVSKAPEKVRDWH
                     KDHDPILNYSRELAGAGVSRADLEKLDLEVSQEIDAAARFALESPLPEAKAALNYAFA
                     GKGN"
     gene            2513..2851
                     /locus_tag="VE170_07455"
     CDS             2513..2851
                     /locus_tag="VE170_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HYJ15310.1"
                     /translation="MNCPKCQTAMLAEFKIEGVTVDRCSACGGTWFDAQELSQLLAED
                     ARQVAELRRGGNDARLDDKRGRCPRDSAELLRMYSAIDRSVTLDACADCHGIWLDAGE
                     FAKLFAARQA"
     gene            3157..>3968
                     /locus_tag="VE170_07460"
     CDS             3157..>3968
                     /locus_tag="VE170_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15311.1"
                     /translation="MRFFKTSACARLFARVLPLCLFILLSSPPMAAPAVRELKEVKVA
                     YPPSMASVTLMTGIKQRFFEEQGLRPVLLVIASDLALKSQVVGEIDYTLFGGGSGMLA
                     AAQGLPIKNVHLPHKFADMTLVARPEFKSVAQLRGKKIGVSSFSGSVYSSTRAMLSAG
                     GLDPDKDVVIIPMGREPIRLQALFAGSIDATPLPVPLHAIAEEKGYSLLADIEGKFEV
                     PFSGITVTDKKLRESADEVKRFIRAMVKAGSFFLSHRSESVALYMDWLKLSKS"
BASE COUNT          769 a         1106 c         1217 g          876 t
ORIGIN      
        1 cgtcgaatgt cccgccggca ctgaaggcga cgatgaagcg caccgtcttg cctttgtagt
       61 agtcgtggct ggaagcatga gccgcgccgg ccacgccgag aacgctgaca gcaagtccaa
      121 ggaggatcgg ccgcaaataa cgcaaagttc gcaaaataaa tattcggcaa atctcccgca
      181 aaggcttgcg gatttcgagt cggtcccgaa tccagattcc tgttttgcgc cctttgcgtt
      241 gtttgcggcc attctcttat tccgattcat cgtcggatca tttgcacagc gcacgggcgg
      301 tttcgacgat ccgttgcgtc attggcgtga catagtcttc cagcggcacg ctgtagggta
      361 ccggtaccgg cgggatcgaa acgcgtttga tcggcgcgcg caagctggtg aagccttcgt
      421 cggcgatgat cgcagccaag ccggcggcga cgccgcagtt gtcatggctt tcgtccgtga
      481 taagtacgcg gccggtcttt ttcacggacg cgagcagcgc cggtttgtcc aacggctgta
      541 acgtccgcgg gtcgatgact tcggccgaga tgccttcttt cgccagcatt tccgccgcag
      601 ccaaagcgcg cggcacttgg atcgaggtgg cgatgatggt caggtcgccg ccggcgcgct
      661 tgatatcggc tttgcccagc ggaatttcat aagcgccgtc cgggacttcg ccgacgtcgt
      721 cgagcagccg atcatggccg aagaacaagg tcgggtcttc gctccgggtc aacaccgtgc
      781 gcataaggcc cttggcatcg gcgggagtcg acggcatgac gatctgtagg ccgggcgtgt
      841 tccagtacca cgactgcgga ctgcccgagt gctgtacgcc gccgccgccg cggatgccga
      901 acttcagata aaaaaccacc ggcacgttgg tctgtccgcc ggagttggag taggcgatgg
      961 cggcttcgtt gacgatctgc gggatcgctt cataggaaaa agtcgcggtg ctgatgtcga
     1021 ccacggggcg caggccggcc atggcggcgc cgatggcgat gccgcaaaag ccgatctcgg
     1081 cgatcggcgg aaattttatt cgcgccgcat atttcttgcg gaacgcggct aacttttcgt
     1141 ggccggcttc gccggcgaag ctcgggttga acagcacgat acgtttgtcc tgctctgcca
     1201 cttcgtcaat tgcggctagc agcgcttcac cgaaggtcat ttcttgcggc atggctagtt
     1261 tccctttccc gcgaaggcgt aattgagcgc cgccttggct tctggcagcg ggctttccag
     1321 agcgaagcgc gccgccgcat cgatttcctg acttacctcg aggtcgagct tttcgaggtc
     1381 ggcacggctc acgccggcgc ctgcgagctc gcgcgagtaa ttcaagatcg gatcatgatc
     1441 cttatgccag tcgcggactt tctccggcgc tttgctgacg tcccacgccc aggagatatc
     1501 ggtcacgcca ccgacaagct tgggcatatt gaacgcgttg cccggccata gatgggtacg
     1561 cgcttcgacg aacagcggtc cttcgcctct gcgcacccgg ccgacgatat ctttcgccgt
     1621 cctgtgcacc gtgccgacat cgatgccgtc gatgacggtg gtctcgacat tcaaggcttt
     1681 cggcacgttg gtaagctccg tcgcagcatg gcccgcgcca cggccgctgc gccgataatt
     1741 attttccatc caaaaaatca tcggcagttg ccacagcttg gcgatattca aagcttcgta
     1801 gaagacaccc tcctccatgg cgccgtcgcc gaaaaagacc acggtaatcg ctttgctgtc
     1861 tttgacttgc gccgcgtatg ccgtgccgcc ggccagcgcg aggcaaccgc cgacgatggc
     1921 ggaggtgtgg atgaccccga cttcaggcgc ggcgacatgg aatgtcccgg cgcgtccttg
     1981 gcagtagccg gtggcgcggc cgatgatctc ggccaggact ttcttcgggt cgccgccctt
     2041 ggcgatgaca tggccgtggt tgcgatgggt ggtgaaaaca tagtcatcgg cattcaacca
     2101 gtcgcacacc gccacgccgg tggcttcctg gccgatatat acgtggaagt ggccgcggat
     2161 cagaccggtg tgttcgtcgt tgaagcggat gcaatgttcc tcggcgcgcc ggatcatcag
     2221 catccggcgc agaatcgcga gctgcttgtc tcgggaaata tcgggtagca tggttcctcc
     2281 gtgggttggt gacctttggt gcattcttac tgccggtatt ggagaattgc aaaggcgtaa
     2341 aaatggataa tcgataagga atcataggta aaccggcgtc attgccggag agttttgcgt
     2401 tggtgggaga ttgaaagttg tcttaaaccg acggctcagg tttgcttgaa ctgcctatag
     2461 ctttgcttta gcgtttacag aacttctgcg ggaaaggtga gtcgtgaaat cgatgaattg
     2521 tccaaaatgc caaaccgcca tgctggcgga attcaaaatc gaaggggtca ccgtcgaccg
     2581 atgttccgct tgcggcggga cttggttcga cgcccaggag ctgagtcagt tgctcgccga
     2641 agatgcacga caagtggcgg agttacgccg cggcggtaac gatgctcggt tggatgacaa
     2701 acggggccgt tgtccgcgcg actcggcgga gctgttgcgg atgtacagcg ccattgaccg
     2761 tagcgtgacc ttggacgctt gtgccgattg tcatggcatt tggctcgatg ccggcgaatt
     2821 cgccaagctg ttcgccgcgc gccaggcttg agaattcaat tgtcggttgg cgcggcgcca
     2881 tgttaaaagc tcaaaggcta tttcagtgga tgttacgaat agtttattaa agcgcgcgcc
     2941 ggtactggcg ctgcgaaaga accggcgcgc cagggttctg aaaacctaaa gttctcctgg
     3001 cagcggaatc cggtttagaa catgtgatca aggtaaccca gcattggcct gttgtttcac
     3061 catgtctgaa tggagtatcc gaccaaagat ccgccggtgc gcatcgctcc aaattcgcat
     3121 atttccacta aacgcaggga aggatgattg aagtaaatga ggttctttaa aacatctgcc
     3181 tgcgctcgat tattcgccag agtcttgccg ctttgcctgt tcattctgtt atcgtcgccg
     3241 ccgatggcgg cgccggcggt gcgcgagctg aaagaggtca aggtcgctta tccgccgtcg
     3301 atggcgtcgg tgactttgat gactggaatc aagcagcgat ttttcgagga gcaagggctg
     3361 cggccggtgc tgctcgttat cgccagtgat ctcgcgctca agagccaagt ggtcggtgag
     3421 atcgactata ccctgttcgg cggcggctcc ggcatgctcg cggcggccca ggggttaccg
     3481 atcaagaatg ttcatttgcc gcataagttc gccgatatga ctttggtggc gcggccggaa
     3541 ttcaagtccg tcgcccagct gcgcggcaag aagatcggcg tgagcagctt ttccggcagc
     3601 gtttattcct cgactcgcgc catgctgtcg gcgggcgggc tcgaccccga caaggatgtg
     3661 gtaatcattc caatgggccg cgaaccgatc cgcctgcaag cgctgtttgc cggcagcatc
     3721 gacgcgacgc cgctgccggt gccgctgcac gccattgccg aagagaaagg ctacagcttg
     3781 ctcgccgaca tcgaaggtaa attcgaagtg ccgttcagcg gcattacggt gacggataaa
     3841 aagctcagag agagcgccga tgaggtgaaa cgatttattc gcgcgatggt caaggccggc
     3901 agttttttcc tgagccatcg cagcgaatcg gtggcgctct atatggactg gttgaaactt
     3961 tcgaaatc
//
LOCUS       DASQKS010000288         6472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2682570, whole genome shotgun sequence.
ACCESSION   DASQKS010000288 DASQKS010000000
VERSION     DASQKS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682570"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VE170_07465"
     CDS             complement(<1..618)
                     /locus_tag="VE170_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYJ15312.1"
                     /translation="MWSLVGYFLKLGTIGFGGPVALVGFMYRDLVERRGWITEDTYKL
                     SLALAQIMPGPLAAQTAIAIGYFEAGIVGATLVGLAFIIPSFLMVVGISFAYVAYGGL
                     WWMQALFYAIGATVIAIIAIAAYKLARSTNKREPLLWAIFAVLTVVTVWAQAELAEFF
                     IVAGLVMLLSHAWPGWRAGSLMTLGAATLAATIWLLEAWLRQTGTT"
     gene            803..1780
                     /locus_tag="VE170_07470"
     CDS             803..1780
                     /locus_tag="VE170_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYJ15313.1"
                     /translation="MKEADDIRWLLLIHQIPPKPSYLRVKIWRRLQRLGAVAIKNSVY
                     VLPKSDQSREDFQWVLREIVEGGGDGTLCEARLVDGINDDEVEGLFQVSRTADYDQIA
                     EDARRLTKTVPPQGKLGKKNRPQLELDLARLKRHLDEAVAIDFFGAPGREAATGLLSG
                     IEARLRDNKAGPTSEGKTNINDLRGKTWVTRTGIHVDRMATAWVVRRFIDPEARIKFV
                     PAKGYTPLHDEIRFDMFEAEFTHENDCCSMEVLMMRTGIRDPGLVRIAEIVHDIDLKD
                     SKFEREETTGIARLIDGIAMAHRDDDARLARATAIFDDLYENFRRKLRP"
     gene            1894..2184
                     /locus_tag="VE170_07475"
     CDS             1894..2184
                     /locus_tag="VE170_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15314.1"
                     /translation="MIVNLFNMSVFAFLVLATPLVYADPSAPSHACHGPGKASEIKTQ
                     PDADKFNDAVNKYRSCIEGFIRQQEEAIKNHQRALSQATSDWNDFVTNNMKQ"
     gene            complement(2468..2692)
                     /locus_tag="VE170_07480"
     CDS             complement(2468..2692)
                     /locus_tag="VE170_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15315.1"
                     /translation="MQQFYKGHTIKTSAQHLRDTQQWQPVLQIIIWNYNQNLFKPFSA
                     TECFSTREQAESEGLVIAKKWIDDGKPGVS"
     gene            complement(3151..3861)
                     /locus_tag="VE170_07485"
     CDS             complement(3151..3861)
                     /locus_tag="VE170_07485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ15316.1"
                     /translation="MANNWANVRVDSSDMPLYVNLPASDRTVPGIVVVHGRSGLEDFI
                     KDTTRMFAQLGYAAVAPSLYHRDGPDCKDDNQVRKARLRDPSIIRDIEGAIGFLRNHP
                     QVDGGRLGIVGFCMGGRLVYLMSAASKDLKAGVMFYGSSTMEPYGEAGPTPFDRTRDI
                     NCPIQGHFGADDQNPSPEDMRKLDAELSKFGKPHEFHSYAGAAHAFVNSGSGNYRPHA
                     AALSWPKAMEFFARHLVG"
     gene            4067..5344
                     /locus_tag="VE170_07490"
     CDS             4067..5344
                     /locus_tag="VE170_07490"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15317.1"
                     /translation="MLSQEDNNLITHTGPGTPGGNLLRRHWHPVALVEELPRDGAPLP
                     VRIMSEDLVLFRGNDGQLGLIGQYCSHRRADLSYGRVEDGGLRCLYHGWLFDRQGRCI
                     EQPCEPPDRRFCEKVRHPAYPCQERAGAVFAYLGPGEPPLLPNYEPLVAPAGHVLASK
                     FYHECNFFQANEGNLDPSHVSYLHRQANVPDNLKRTVTGSDGKLPLALYDADMAPRID
                     AEETDYGVRIFSLRDVDGRTFFRVTNYVFPNKATIPGPMSGDGYNLYWHVPIDDTHHW
                     RYDFVFRRSAPMEQRDIERDREILDELGPGYRPKRNKSNRYLQDREAMKNWSFSGMGR
                     IFNVHDVAIVEGCGEIVDRSKEFLGPSDKAIIVARRQLLRAIRDVEAGREGPHVIRAP
                     DANRFPQLTVESEVLAPGADWQEHWKSRLEVLR"
     gene            complement(5440..6396)
                     /locus_tag="VE170_07495"
     CDS             complement(5440..6396)
                     /locus_tag="VE170_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15318.1"
                     /translation="MKIFVVLLLLLAAAVPCQARKVSVAIPVVDVTQSALYVARDRGY
                     FHKEGLEVDFVLMRGGLANQALITGNVDFTTVPTAALQAGLQGAPVKVLLSTFHKPMF
                     WLYARPAIRTAKDLSGKKVAVSSLGAAGDSALREWLKNNGMDENREVAILAIGTTATR
                     LASLATGVVDAAMMTFPHNITAAEQGFRELISFIASDIIQLQGAIVTHDGLLKSDPST
                     VEKFLRATIRGLIYYSTNRSGAVPILARNARSSEDLAGKVYDLVKPALTPDGILNDDL
                     QKRVMSPLLERVGRKDVAIARFFDFALARKINAELKAEGAKP"
BASE COUNT         1535 a         1881 c         1763 g         1293 t
ORIGIN      
        1 cgtggtgccg gtttgacgca gccaggcctc aagcagccaa atcgtagccg ccagagttgc
       61 cgcgcccaat gtcatgagcg aaccagctcg ccaaccaggc catgcatggg agagcagcat
      121 gacaagaccg gcaacaataa agaactccgc cagctcggcc tgcgcccata ccgtaacaac
      181 cgttagaacg gcgaaaatag cccaaagcaa cggctcacgt ttattggtgc tgcgcgcaag
      241 cttataggcc gcaatagcga tgatcgcaat gacagtggcg ccgatcgcat agaaaagcgc
      301 ctgcatccac cagagaccgc cgtaagcgac ataggcaaac gaaatcccga cgaccatcaa
      361 aaacgacggg ataatgaatg cgagaccaac cagcgtggcg ccgacaatgc ctgcctcgaa
      421 atatccgatg gcaatcgccg tctgagcagc cagcggcccg ggcataatct gcgccagcgc
      481 gagggaaagc ttgtaggtat cctcggtgat ccaaccgcgc cgctcgacta ggtcgcggta
      541 cataaaaccg accaatgcca ctggtccgcc aaagccaata gtgcctagtt tgaggaagta
      601 gccgacgagt gaccacaagc ggatttgtgg atcgggcgct attactgccg cgctggctgc
      661 aactcgcgtt tgccggccta aaggttccat caaggaacac tctctgtttc agttaacggt
      721 cgagacggta tagactaacc atcgtgtata gggacgactg tatcatatat ataacaagtg
      781 tttctcgtgt caaggatggc caatgaaaga agccgacgac atccgctggt tattattgat
      841 tcaccagatc ccgccaaagc cgagttacct gcgcgtcaaa atttggcgcc gcctgcaacg
      901 gctcggcgcg gtagctatca aaaattccgt atacgtcctt cctaaaagcg atcagtcgcg
      961 agaagacttc caatgggtat tgcgcgagat cgtcgaaggt ggcggagacg gcacgctgtg
     1021 tgaggcgcgg ttggttgatg ggattaacga tgatgaagtc gaaggactgt tccaagtatc
     1081 tcgcacggcc gattacgacc agatcgccga ggatgcacgc cggctaacca agactgtccc
     1141 tccccaaggg aagctcggta aaaagaatcg gccgcagctt gaactcgacc tcgcgcgact
     1201 aaagcgccac ttggatgaag cggttgccat cgatttcttt ggcgcgcccg gccgtgaggc
     1261 ggcaactggg ctgctctccg gcatcgaggc gcgcttgcgc gacaataaag ctggcccgac
     1321 atccgagggc aaaacaaaca ttaatgattt acgaggtaag acttgggtaa cccgaaccgg
     1381 tatacatgta gatcgaatgg ctaccgcctg ggtagtccgg cgcttcatcg atcctgaagc
     1441 tcgcatcaag ttcgttccag ccaaaggata cacaccgcta catgatgaaa tccgattcga
     1501 tatgtttgag gcggagttca cacatgagaa cgactgctgt tcaatggaag tattaatgat
     1561 gcgaacgggg attcgtgatc ccggccttgt taggatcgct gaaatcgtcc acgacatcga
     1621 cctgaaagac tcgaagtttg aacgggaaga aactacaggc atagcccgac tgatcgatgg
     1681 aatcgctatg gcacatagag atgacgacgc gcggcttgcg cgggccactg cgatattcga
     1741 tgatctctac gaaaactttc gcaggaagct tcgtccttag atctcacact gctccttccc
     1801 acttgcatgg tgttgccaag cagcgcgcag ttgtcccgtt gacaagtaga cagcagaata
     1861 tcatgcgata aagtcatatc tccaggagca accatgattg taaacctttt caatatgtcg
     1921 gtttttgcct ttttggtgct tgccactccg ctcgtctatg ctgacccttc tgccccatct
     1981 cacgcatgtc atggtccagg taaggcgtct gagattaaaa cacaaccaga cgcagacaaa
     2041 tttaacgacg ctgtgaataa gtatagatca tgtatcgagg gatttatccg tcagcaggaa
     2101 gaagccatca aaaatcacca acgcgccctg agtcaggcga ctagcgactg gaacgatttt
     2161 gtgactaaca atatgaaaca gtaacctgcc gccggcgctt gttgagtcta tagcctgtcc
     2221 cgcttcaaca cctcgcggcc gacttcggtg atgagcggga tcataaccaa aattacgagc
     2281 acgaccgccc acggcccgac gtaaccgcta cgatacccga gcacgccggt aacagcgagc
     2341 gcggcaagta ggaatagaat aaaccgaata gcgtatggct tgcaccgttt acagcacgtg
     2401 catggataac tcaaggcggg agaattgacg ccggagaagc cgccgatgat ctcgtggcaa
     2461 gagcggacta ggagacgccg ggcttgccgt catcgatcca ttttttagcg attactaggc
     2521 cttcactttc agcttgctcc cgtgttgaaa aacattcggt tgcgctgaaa ggtttgaata
     2581 agttctggtt gtaattccaa attattattt gaaggaccgg ttgccattgt tgcgtgtcgc
     2641 gtaaatgctg agctgaggtt ttaatcgtat gacctttgta aaattgttgc ataacgctcc
     2701 ctcaaaggag ctttttttac cgcacatcac agagataaag caaccgtacg cttgggattt
     2761 aattacgtgg gagcggataa taagtagcag ccacatgaag aaggcataca gagattacac
     2821 aatcgacatc atcatagcgc ggcagatggc tctggaaggc gaatgtttga atgggcccga
     2881 gattcgaaat cccgagagaa ccctgcatga tgtgagcgaa ttagaaggcc acaacagccg
     2941 atgagagacc aaacaagcag ctattcggcg ggctgaggaa cagatcaatg cacacgtcga
     3001 aaataggtcc cactctctag tttcaaacgg cgtcgcgcac aatcaaaata ttctagtcaa
     3061 agttttcgca attaatcgcg attctggtcg caattcgcaa caacgagttg aacggcttga
     3121 acgttttgaa cgattggaac gccgtctccg ctaccccacc aaatgccgtg caaaaaactc
     3181 catcgccttg ggccaagaca gcgccgcggc atggggccga taatttcctg agcctgaatt
     3241 gacgaacgcg tgcgccgcgc cggcgtagct gtgaaattcg tggggcttgc cgaacttgct
     3301 cagctcggcg tcgagcttgc gcatgtcttc cggcgaggga ttttgatcgt cggcgccgaa
     3361 atggccttgg atcggacaat tgatgtcacg cgtgcggtcg aacggggtcg gtccggcttc
     3421 accgtaaggt tccatggtgc tgctgccgta gaacatcacg ccggctttca aatccttgct
     3481 tgccgccgac atcaaataaa ctaaccgccc gcccatgcag aagccgacga tgccgagcct
     3541 gccgccgtcg acctgcgggt gatttctgag aaagccgatc gcgccttcga tgtcacgaat
     3601 gatcgacgga tcgcgcaggc gcgccttgcg cacttggttg tcatctttgc aatccggccc
     3661 atcgcgatga tagagactcg gcgccaccgc ggcataaccc agctgggcaa acatccgcgt
     3721 cgtatccttg ataaaatctt ccagacccga gcggccatgg acgacaacga tgcccggcac
     3781 ggtccgatcg ctcgccggca gattcacgta aagcggcatg tcgctgctat cgactcgaac
     3841 atttgcccaa ttgttagcca tggtcgcccc cgttcatttc acgtcacttt tctattgaat
     3901 tgaagaccgc tctgcaacct gttccggtat tagctacaaa gacgatcgcc agtcgccgcc
     3961 agataaaatg gcggaccaac ggtgattgcg accaacgcag gaatgatgat aaaacgactt
     4021 cgataattga aaagcaggcc tccagactaa aatcggaaat gaccagatgc tttcccagga
     4081 agacaacaac ctaatcactc acaccggtcc cggcacgccg ggaggaaatt tgttgcggcg
     4141 ccattggcac cctgtcgccc tcgtcgaaga gctaccgcgc gatggcgcgc cgctgccggt
     4201 caggatcatg agcgaagatc tggttctgtt ccgcggcaac gacggccagc tcggtttgat
     4261 cggccaatac tgctcgcacc ggcgcgccga cttgagctat ggccgcgtcg aagacggcgg
     4321 cctgcgctgc ctgtatcatg gctggttgtt cgaccgtcaa ggccgctgca tcgagcagcc
     4381 ctgcgagccg ccggaccggc gcttttgcga gaaggtgcgc catcccgcct atccctgcca
     4441 ggaacgggcc ggcgctgtct tcgcctacct gggaccgggc gagccgccgc tcctgcccaa
     4501 ctacgaaccg ctggtggcgc cggccggcca tgtgctggcc tccaagttct atcatgagtg
     4561 caatttcttt caggccaacg aaggcaatct cgaccccagc catgtttcct atctgcaccg
     4621 ccaagccaac gtgccggata atttgaaacg caccgtcacc ggcagcgacg gcaagctgcc
     4681 tttggcgctc tatgacgccg acatggcgcc gcgcatcgac gccgaagaaa ccgattacgg
     4741 cgtgcgcatc ttttccctgc gcgatgtcga cggccggact tttttccgcg tcaccaacta
     4801 cgtgtttccc aacaaggcga cgatcccggg cccgatgtcg ggcgacggtt acaatcttta
     4861 ctggcatgtg cccatcgacg acacgcacca ttggcgctac gacttcgtgt tccgccgcag
     4921 cgcgcccatg gagcagcgcg acatcgagcg cgatcgggaa atcttggacg agctcggccc
     4981 cggctaccgg cctaaacgca acaaaagcaa ccgctacctg caggaccgcg aagcgatgaa
     5041 gaactggagc ttctccggca tgggacggat cttcaatgtg cacgatgtcg ccatcgtcga
     5101 aggctgcggc gagatcgtcg accgcagcaa ggaattccta ggccctagcg acaaagccat
     5161 catcgtcgcg cgccgccagc tcttgcgggc gatccgcgac gtcgaagccg gccgcgaagg
     5221 gccccatgtc atccgtgcgc cggatgccaa ccgcttcccg caactcaccg tggagtccga
     5281 agtcctcgcc cccggcgccg actggcagga gcactggaaa agcaggctcg aagttctgcg
     5341 ctaattgtct ttaagcgaaa atggcggcgg cccggcgctc cgaagacgtt ttgaacggct
     5401 tgaacgtagc gtagcgattt aacgacttga acgatcggcc tacggcttcg cgccctcggc
     5461 cttgagctcc gcattgatct tgcgcgctag agcaaagtca aaaaacctgg cgatagcaac
     5521 atccttgcgc ccgacccgct ccaacagcgg gctcatgacg cgcttttgca aatcgtcgtt
     5581 gagaattccg tccggcgtca gcgccggttt gaccagatca taaaccttgc ccgccagatc
     5641 ttccgacgac ctggcgttgc gcgccaggat tggcacggcg ccgctacgat tggtcgagta
     5701 ataaatcagc ccgcgaatcg tcgcgcgaag aaacttctcg accgtgctcg gatccgattt
     5761 tagcaagccg tcatgggtca cgatggcgcc ctgtaactgg atgatatccg aggcgatgaa
     5821 cgatatcagc tcgcgaaagc cctgctcggc ggcggtgata ttatgcggaa aggtcatcat
     5881 cgcggcgtcg accaccccgg tggcgagaga ggcaagccgc gtcgccgtgg tgccgatggc
     5941 gaggatcgcc acttcgcgat tttcatccat gccgttgttc ttgagccact cgcgtagcgc
     6001 cgagtccccc gccgcgccca agctcgacac cgcgaccttt ttgccgctca gatccttggc
     6061 cgtgcgaatc gccgggcgcg catacagcca gaacatcggc ttgtgaaaag tgctgagcaa
     6121 tacctttacc ggcgcgccct gcagacccgc ttgcagcgcc gccgtcggca cggtggtgaa
     6181 atcgacattg ccggtgatca acgcctggtt cgccagcccg ccgcgcatca atacgaagtc
     6241 gacttcgagc ccttccttgt gaaagtagcc gcggtcgcgc gcgacataaa gcgcactctg
     6301 ggtcacgtcg acgaccggaa tagccaccga caccttgcgc gcttggcaag gcaccgcggc
     6361 ggcgagcaga agcaacaaaa caacgaaaat tttcatcagg ttaattcctc acacggccaa
     6421 catcgccgga tgatacacgc tgggtcaaac cggctcaagg cgttgacctg cg
//
LOCUS       DASQKS010000289         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2254967, whole genome shotgun sequence.
ACCESSION   DASQKS010000289 DASQKS010000000
VERSION     DASQKS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254967"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VE170_07500"
     CDS             <1..587
                     /locus_tag="VE170_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15319.1"
                     /translation="DRRLLSLILLTYGIALVGTPYSRFLPVFATDVLHAGPTTFGLLL
                     AAPGVGAVLAGLGIASLGRLRRRLHFVAMAVLAFSVSLMLFSFSRSIYLSTLFLILVG
                     ASNIAFRAVANSIVQMETPPQYLGRMLSLFFMDKGLWSFGTLFIGSVAHLIGTPRAIT
                     ISGAVCAIAAAVLLHQQRQARGGEPARSLDQRVA"
     gene            complement(567..1556)
                     /locus_tag="VE170_07505"
     CDS             complement(567..1556)
                     /locus_tag="VE170_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HYJ15320.1"
                     /translation="MSEFEQDPLTDRIVIIAPERAQRPLRFGPPSSAESVQPCPFCPG
                     NEQLTPPESWADRPPDSPPDRPGWSIRVVPNKYPAVTAAGAGKDQTADRSPGIGIHEV
                     IVENPAHVVNLAALDEAQFERIFHAYRERLRALRGDHRWRYFLIFKNQGERAGATLDH
                     VHAQLLALPFVPADVEKELAGAREYHRRSSRCFYCAMVERELQAQARLVTSTAAFIAL
                     CPEAPRFAFETWILPRLHGAVFDEADDSSVAALAQISRQVIGALDRLRANPPFNYFIQ
                     SLPLADNERAQYHWQLRLLPQFSRAAGFEWGSGIHINPVAPEVAARRLRDALI"
     gene            complement(1559..2173)
                     /locus_tag="VE170_07510"
     CDS             complement(1559..2173)
                     /locus_tag="VE170_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HYJ15321.1"
                     /translation="MVYTPQDRYFKKAKQDGYRSRAAYKLLELQQRFRLINPGDRVVD
                     LGAAPGGWLQVAAKLVGPNGKVIGIDVQAIQPFQERNIELIQGDILTGATQCKIKELL
                     GGPAHCVLSDMAPRLSGIRDADMARSYELNQMALKVAKELLRPGGILLVKSFISEDLQ
                     TYTADLKNHFAQVQRTKPEASRQGSSEFYFYANGYAAKTNAVVS"
BASE COUNT          437 a          687 c          680 g          503 t
ORIGIN      
        1 gcgaccgccg gctgctctcg ctgattctcc tgacctatgg catcgcgctg gtcggcacgc
       61 cctattcgcg ttttttaccc gtgttcgcca ccgacgtgct ccatgccgga ccgacgacct
      121 tcggtttgtt gctcgccgcg cccggcgtcg gcgccgtcct cgccggcctg ggcatcgcat
      181 ctctcggccg attgcgccgg cgccttcatt tcgtcgccat ggcggtgttg gcgttctcgg
      241 tttccctgat gctcttttcc ttttcgcgct cgatctatct ttccacgctg tttctcatcc
      301 tggtcggcgc cagcaacatc gcttttcgcg ccgtggccaa ctctatcgtt caaatggaaa
      361 cgccgccgca gtatctcggc cgcatgctca gtctgttttt catggataag gggctgtggt
      421 cctttggcac cttgttcatc ggcagtgtcg cccatctgat cggtacgccg cgggcgatta
      481 ccatttccgg tgccgtttgc gcgattgccg ccgcggtgct gttgcaccaa caacgacaag
      541 cacgcggcgg cgagcctgct cgcagcttag atcagcgcgt cgcgtaatcg ccgcgccgca
      601 acttccggtg ccaccggatt gatatgaatc cccgaacccc attcgaatcc ggcggctcga
      661 gagaattgcg gcagcaagcg caactgccaa tgatactggg cgcgctcatt gtcggccaaa
      721 ggcagcgact gaatgaaata attgaacggc ggattggccc gcaaccggtc caaggcgccg
      781 attacctgcc gcgagatttg cgccagagca gccacactgg agtcatcggc ttcatcaaag
      841 acggcgccat gaagccgcgg cagtatccag gtttcaaaag cgaagcgggg cgcttcggga
      901 cagagcgcga tgaatgctgc cgtgctcgtg acgagacgcg cttgtgcctg cagctcgcgt
      961 tcgaccatgg cgcaataaaa acagcgggag ctgcgccgat gatattcacg ggcgccggcc
     1021 aattcctttt ccacatcggc ggggacaaac ggcaaggcga gcagctgcgc atgcacatga
     1081 tctagtgtgg cgccggcgcg ctcgccttgg tttttgaaga tcaagaaata acgccagcgg
     1141 tgatccccac gcagggcgcg caaacgctca cgataggcgt gaaaaatccg ctcgaactgt
     1201 gcttcatcca gcgccgccag attgacgaca tgagccgggt tttcaacgat gacttcatgg
     1261 atgccgatgc cgggggaccg gtctgcggtt tggtctttcc ctgcgcccgc agcagtgacc
     1321 gccggatatt tgttcggcac cacgcgaatc gaccagccgg gccggtcggg cggcgaatcc
     1381 ggcggccgat ccgcccatga ttccggtggc gttagctgtt cattgccagg gcaaaatgga
     1441 cagggttgga cagactccgc gctactgggc ggaccaaacc gtaagggccg ttgggcgcgc
     1501 tcgggcgcga tgatcacgat gcggtcggtg agcgggtcct gttcgaattc cgacatactt
     1561 aggatacaac cgcgttagtt tttgccgcat agccgttggc atagaaataa aactcggacg
     1621 aaccctggcg gctcgcttcc ggcttagtgc gttgcacctg ggcgaaatga tttttcaggt
     1681 cggcagtgta tgtctgcaga tcttcactga taaaactctt gaccagcaat atgccacccg
     1741 gacgcaatag ctccttggcg actttcagcg ccatctggtt gagttcgtag gagcgtgcca
     1801 tatcggcgtc acgaataccg ctcagtctcg gcgccatatc ggacagcacg caatgcgccg
     1861 gtccaccgag taattctttg atcttacatt gagttgcacc ggttaaaata tcgccctgaa
     1921 tgagttcgat gttgcgctct tgaaaaggct ggatcgcctg cacatcgatg ccgatgactt
     1981 tgccgttagg accgacgagc ttggcggcga cctgcaacca accgcccggc gccgcaccca
     2041 ggtcgacgac tcggtcgccc ggattgatca agcgaaagcg ctgctgcagt tcgagaagtt
     2101 tataggcggc gcgggagcga taaccgtcct gtttggcttt cttaaaatag cggtcttggg
     2161 gtgtgtaaac catgtcggcg gttgtgcggc ctactttatc cgatcggcgc caataaaaaa
     2221 gcccagccga aagaacggct gggcttggtg acgatgagtc gatggattgc gctactgcgc
     2281 cgatttcacc tggaattcga cgcggcg
//
LOCUS       DASQKS010000290         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4771410, whole genome shotgun sequence.
ACCESSION   DASQKS010000290 DASQKS010000000
VERSION     DASQKS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4771410"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VE170_07515"
     CDS             complement(<1..247)
                     /locus_tag="VE170_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit alpha"
                     /protein_id="HYJ15322.1"
                     /translation="MGTIAGAQNILAGLKGAGIDLVASVPDINMLQLLNLLYEDKEIA
                     HVPVGREEEGIGVCTGAHLAGKFPAMLMQNGGLLNSAN"
     gene            396..1355
                     /locus_tag="VE170_07520"
     CDS             396..1355
                     /locus_tag="VE170_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYJ15323.1"
                     /translation="MIKFVWKIVALAAVMFAAPVAQAQYPAKTIQIINPFPPGAVTDI
                     VARILAPKMSAALGQQVVIVNKAGGGGAVGIQAAKDAAPDGYTILVTPPPIALIPIVN
                     KNSSFTLKDFAPLALATSSPNTTVVKGDAPWKTLEEFIADAKKNPGQLTYGSAGPGTT
                     PHFIGELVKLKTGIDMTHVPLGSESAAATAVLGGHVSVGFLTLGTTRSHVEAGTMRAL
                     AVASNRRLKDFPNIPTTVEKGHPELNLKVWVGFFAPAKTPTAAVKRLVAAISDSLKDA
                     EVTANIEKAQALIENLGPGEFAKFLAEEERKWSEVARAAKITQ"
     gene            1391..1846
                     /locus_tag="VE170_07525"
     CDS             1391..1846
                     /locus_tag="VE170_07525"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ15324.1"
                     /translation="MKRLDVAAAAALAALAAYVLFESTKLRAGSFRMPQTGFFPRILG
                     VLLILLTLVELFRALRQPASADAPGKIVSDGWIRIGATLATMLGFAVVLERLGFLLAT
                     FFLMVLLLRTIEAPRWPKVFIVSLVASLLSYGLFALLLGVPLPAGILGF"
     gene            1848..3329
                     /locus_tag="VE170_07530"
     CDS             1848..3329
                     /locus_tag="VE170_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ15325.1"
                     /translation="MELLQNFALGLSVALTPMNLLFCFIGALVGTLVGVLPGLGPVGA
                     VAFLLSITFKMEPASAIIMLAGIYYGAMYGGSTTSILINIPGETASVVTCLDGYKMAR
                     QGRAGAALGISAFGSFIAGTVGVIGLMLLAPMLGRAALSFGPAEYFALTLTGLTLVAY
                     LSQGSMIKALIMAVLGLIAGTVGMDPISGEERFTYGTLTLRDGFGLVPVAMGLFGIAE
                     VLENLLVRGSQSVYETKIQGLLPNRQDWRDSAAPIARGSALGFFLGILPGPGPVIASF
                     VSYAMEKRLSRYPEKFGTGVIEGVAGPEAANNAATAASFIPLLSLGIPTSAIMALFMG
                     ALMIHGIQPGPLFIARYPELFWGFIASMYVGNAMLLILNLPLIGIWVRLLKTPYAILF
                     PLILLFCLIGVYSLNANVAEIVIMLAFGVAGFVLRRFGYEGAPFILAMVLGPIMETSL
                     RQTLLISRGSFAIFLTRPICGVLIGLTVAFLIWPIFMRKKKDA"
BASE COUNT          650 a         1038 c         1054 g          819 t
ORIGIN      
        1 cgttggcgct gttgagcagg ccgccgttct gcatcagcat ggcggggaat ttgccggcga
       61 gatgggcgcc ggtgcaaact ccgatgcctt cttcttcacg ccccaccggc acgtgggcga
      121 tctctttgtc ttcgtaaagc agattaagca gttggagcat gttgatgtcc ggcacgctcg
      181 cgaccagatc gatgcccgcg cctttgaggc cggctaaaat attctgcgct ccggcgatgg
      241 ttcccatatt cgattaacct ctggtcggtt ctaggcaacc cgacggatgc tgtcaaggtc
      301 ctgcggaaaa gattgacact tccccggcga ttctgttagc aggaagtgcg gcgagaggaa
      361 agcaaacaag caaaactctg ccaaagcgag gtatcatgat caagtttgta tggaaaattg
      421 tcgccctggc tgcggtcatg ttcgccgccc cggtggcaca ggcgcagtac ccggccaaga
      481 cgattcagat catcaatccg tttcctcccg gcgcggtgac cgatattgtc gcgcgcattc
      541 tcgcccccaa gatgtcggcg gcgttggggc agcaggtggt gatcgttaac aaagccggcg
      601 gtggcggcgc ggtcggcatt caagcggcga aggacgcggc gccggacggc tacacgattc
      661 tggtcacccc gccgccgatc gcgctgatcc cgatcgttaa caagaattcc agtttcacct
      721 tgaaagattt cgcgccgcta gcgctggcca ccagcagccc gaacaccacg gtggtcaagg
      781 gcgacgcgcc gtggaaaact ttggaggagt ttatcgcaga tgctaagaag aatcctgggc
      841 aactcaccta cggctcagcc ggtccgggca cgacgcccca tttcatcggc gagttggtga
      901 agcttaagac cggcatcgac atgacgcacg tgccgctggg ttccgagtcg gcggcggcga
      961 cggcggtgtt gggcggtcac gtcagcgtcg gctttctcac tctgggtacg actcggagcc
     1021 atgtagaagc cggcaccatg cgcgcgctgg cggtagcgtc caatcgccgc ctcaaagatt
     1081 ttcctaatat tcccaccact gtcgagaagg gccatccgga actgaatcta aaagtttggg
     1141 tcggcttctt tgcgccggcc aagacgccga cggcggcggt gaagagactg gttgcggcga
     1201 tcagcgatag cttgaaggat gccgaagtca ccgccaacat tgagaaggct caagccttga
     1261 tcgaaaacct cggccccggc gagtttgcca agttcctcgc cgaagaagag cgcaagtgga
     1321 gcgaagtggc gcgcgccgcc aaaattaccc aataattgcg ttgttattca ttcgcaccgt
     1381 tgcgccacgc atgaaacgac tcgacgtcgc cgccgccgca gcgctcgccg cactggccgc
     1441 ctatgtcttg tttgaatcga ccaagctcag ggcgggcagt ttccgcatgc cgcagactgg
     1501 ttttttccca cggatccttg gagttctatt aatcctgctc actctggtcg agctgtttcg
     1561 cgcgttacgc cagcccgcaa gcgctgacgc ccccggcaag atcgttagcg atggctggat
     1621 tcgcatcggt gctacgctgg cgaccatgct cggcttcgcc gtggtactgg agcggttggg
     1681 ctttttgctc gcgacatttt ttctgatggt gttgctctta cgcaccatcg aggcgccgcg
     1741 ctggccgaaa gtctttatcg tttcattggt cgcgtcactg ctttcttacg gcttattcgc
     1801 cttgctcttg ggcgtccccc tgcccgcggg aattttagga ttttaaggtg gagctgttgc
     1861 aaaatttcgc cctgggtttg tcggtggcgt tgactccgat gaacctgctg ttctgcttta
     1921 tcggcgcttt ggttggtact ttggttggtg tgttgccggg cctgggtccg gtgggcgcgg
     1981 tggcgttttt actgtcaatt accttcaaaa tggagccggc cagcgcgatc atcatgctcg
     2041 cgggaattta ttacggcgcc atgtacggcg gttcgacgac ttcaatccta atcaatattc
     2101 ccggcgagac cgcgtcagtc gtcacctgtc tcgatggtta taaaatggcg cggcaaggac
     2161 gggctggcgc ggcgctgggc atctccgcct tcggctcatt catcgccggt accgtcgggg
     2221 tgatcggcct gatgttgctg gcgccgatgc tcggcagggc ggcgctcagc ttcggccctg
     2281 cggaatattt cgccctcaca ctcaccggtc tgaccttggt cgcttatctg tcgcaaggtt
     2341 cgatgatcaa agcgttgatc atggcggtgc tcggcctgat cgccggcacc gtcggcatgg
     2401 acccgatctc cggcgaggag cgttttacct acggcacatt gactctacgc gatggcttcg
     2461 gcctggtacc ggtggcgatg ggattgttcg gcatagccga agtgttggaa aatttgcttg
     2521 tccgaggcag ccagtcagtc tatgaaacca agattcaagg attgctgccc aaccgtcaag
     2581 attggcgcga cagcgcggcg ccgatcgcgc gcggttcggc gctcggattt tttctcggca
     2641 ttcttcctgg gccaggtccg gtgattgcgt cgttcgtttc ctatgccatg gaaaaacgct
     2701 tgtcgcgcta tccggaaaag ttcggcacgg gcgtcatcga aggcgtggcc ggtccggaag
     2761 cggcgaacaa cgccgccacc gcggcttctt tcattccact gctgtcgctg ggcatcccca
     2821 ccagcgcgat catggcgctg ttcatgggcg cgctgatgat ccacggcatc caaccaggcc
     2881 cgctgttcat cgccagatat ccggaactgt tttggggctt tatcgccagc atgtacgtcg
     2941 gcaacgccat gctgctgatt ctcaacttac cgctgatcgg catctgggtg cggttgttaa
     3001 aaacgcccta cgcgattcta tttccgttaa tattactatt ctgcttgatc ggagtttaca
     3061 gcctcaatgc caacgtcgcc gagatcgtca ttatgctcgc cttcggcgtc gccggcttcg
     3121 ttttgcgccg cttcggctac gaaggcgcgc catttattct cgccatggtg cttgggccga
     3181 tcatggagac gtctctgcgc cagacgctgt taatctcgcg cggcagtttt gccatttttc
     3241 tgacccggcc gatctgtggc gtgctgattg ggctgacggt agcgtttttg atttggccga
     3301 tatttatgag aaagaaaaaa gacgcctaat cacaaagtac caagggtcga gcgcggcacc
     3361 gcggcaaccg aagcagattt agccgccgtg ctcttgccaa ggatctcgcc gtccagttgc
     3421 tcctatgtcc cgccagtctg tggcatccga tagcgccgca gcacgggtat ccggccgtta
     3481 ccgccaccag aatcatcagg gtcgccagcc cacctgtgag cgccgacatt cgggcaccaa
     3541 gccaagcggc gacgagaccg g
//
LOCUS       DASQKS010000291         7048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1005981, whole genome shotgun sequence.
ACCESSION   DASQKS010000291 DASQKS010000000
VERSION     DASQKS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005981"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VE170_07535"
     CDS             complement(<1..726)
                     /locus_tag="VE170_07535"
                     /inference="COORDINATES: protein motif:HMM:NF025958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HYJ15326.1"
                     /translation="MSDALQWLDPRDQRFDWLNVADWEPQGDDGFQPVRVTKAWRDQW
                     PQKTARRARSSAGMTVRFRTDSKKLVFRVTFVDAPDTPPATAAAGWERSRPNFFALYR
                     DGKYVASMAGLTHFERQEVTIFNDRELAGAAEIQVLLPFYYRNSEIILYAIGVDAGAH
                     LEPAVPDKRPRIFFHGDSITQGHGVTSPRETYVWQAAEQLNCVGINFGFGGTAWADNI
                     VAQTIASRNDWDVLSIMLGTNSLG"
     gene            complement(754..1797)
                     /locus_tag="VE170_07540"
     CDS             complement(754..1797)
                     /locus_tag="VE170_07540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15327.1"
                     /translation="MRFGTFSYNQRRPWASEKQAFDELLDQIELTEKLHFNEAWFAEH
                     HHSDYGMLASPNLMIAALARRTERLRFGNLVNVLPLYDPMRLAEECAMLDILTGGRLN
                     IGLGRGVPKDDMKHRLDRETAQARFEEGIEILLRAWTGETFSYTGKAWGYEEISCRPQ
                     PLQKPHPPIYYGATSPDSPAMVARRGWNLALSRQPLANCAKAIEKYRAELAGLGHVNG
                     NGDAIMVRDIYVADTDEQAWREAVPEIMRFWQLATDNYWRGETVAEADLTRFTERYAY
                     YPGVLTIKRLDEWGTSLIGSPDTVVKKARAMIATAKPDSLVGMFQFGGLKHEQVMHSL
                     ELFGTKVMPALGV"
     gene            complement(1883..2866)
                     /locus_tag="VE170_07545"
     CDS             complement(1883..2866)
                     /locus_tag="VE170_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15328.1"
                     /translation="MIARVVLLILFIFAAVAVTQPGNAAQPAVETIRIGIPGKLVDFA
                     PFFVGIKSGIYRSEGLEPQFIVMRSGIIIPALLSGELDYTTIYGSTIRSAVSGLPLKI
                     IATLITKQSFFLYAHPDIKRVQDLKGKRIAISGFASSTDKAAREALKPAGLEALRDVT
                     LMAMGDTSVRFQALLSGAIEAAILTPPYTVMAEQKGMRNLVWLGDLLGDTPSNGLSTS
                     AKKLKERPDQVQRFLRASVRSMIYTREHKQEALLVLMKEFPGLERNTLAGTLDFYLKA
                     MSLDGRIGDAVVQDLINEQRELLGVKTEVALSQVADFGPLNRAVKELAAGK"
     gene            2976..3599
                     /locus_tag="VE170_07550"
     CDS             2976..3599
                     /locus_tag="VE170_07550"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYJ15329.1"
                     /translation="MTAVNKIDVPAEHWDKLFAPSACLVMITTVDDAGRVNAASYGTC
                     VRVCHDPVYIAFTTGSTKDTYNNILANGEFVVNVPSHEREILEKVRVVGLEFPPGVNE
                     LEKAGLTAIPAKVVKPPRIADCRSHFECKVEWTKQWLHRLMVVGKVVGASVDDGCVDD
                     KGYILWDKVKPAHYCGHEYKNGFVAAYQPMWVDMIYNEPIPPRPAGK"
     gene            3768..4490
                     /locus_tag="VE170_07555"
     CDS             3768..4490
                     /locus_tag="VE170_07555"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15330.1"
                     /translation="MFESWRGVVGDIKPTHRPGSLEEFIKLLPEGIGVIPLTVGISSG
                     TEKEFHDVLADYQQKAAQLAELGVDLISIGGAPPMMVHGYKGEEKIVTDLEAKHGIPV
                     NTSGRSTAAAMTALGIKNFVGVTYFPERMNQIFSRYFTEAGFQVAAMEGIDVPFKDVG
                     KLSGEEIYKHTKKAFLKHAGADAIYMLGSGWRLLKIVRLLEQDLQVPVVYALGAKLWE
                     VQKRFHVREPIKGYGRLLEELP"
     gene            complement(4565..5605)
                     /locus_tag="VE170_07560"
     CDS             complement(4565..5605)
                     /locus_tag="VE170_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15331.1"
                     /translation="MAIFRSLMTVTLLLVSLDPVNQAGAQQAMPTIDQAKKEGEVVWY
                     GTLTGGAIVGQIIKNFENKFPGVKVKYLRLGGGSFVERIRSEARAGKYLWDVVTSEYI
                     QFSELSKHVALARYASPDAAQFSPRHRDPNGTWVSIYGAVATIAWNTRMVKPAEAPKD
                     WKDLLDPRWKGKKIGLPVEAFQWYGGMVTYLGDKAGRDFMKALAAQDPQTQQGYTNTS
                     NLLVAGEFPVAITRAHRIEDARSKGAPVDWSAEANPIVISIHPVGISEKATHPAAARL
                     FYNFLASVEGQSLFTEEGFTSSHSGVKARFPRLTLDNIKHPAPPDPKVSERIQTWISE
                     HQQTFRLAARGG"
     gene            5843..6871
                     /locus_tag="VE170_07565"
     CDS             5843..6871
                     /locus_tag="VE170_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15332.1"
                     /translation="MNQLAAQDNVELLDRDMAGKNLSGYWRLGMEGLPDYPVTAVKPC
                     LWKWQDIHDSLVRAGEVISLENSERRVVRLVNPGLDRKHAFATHTIQVSFQYVKPGEN
                     ARAHRHTPAALRFVVEGNGAYTTVNGQQCVMEPGDLILTPKLTWHDHSNDSKIPMLWL
                     DGLDFPLVTALHQVMQERYPERRQAIETNSESGIGGASGAVSGGRPLAEFFHYKWRDI
                     EPGLRALVKSANARDRFDGYLFEYRNPLTGGPTMTTMQCAVQLLSAHEETDAHRHTST
                     VMYHVFRGSGTTEVGEQRFDWQQGDTFVVPLWHSHRHSNGSSDEAILFSISDAPALKA
                     LGLYREEF"
BASE COUNT         1440 a         1968 c         2131 g         1509 t
ORIGIN      
        1 gccgagtgag ttagttccga gcataatcga cagcacgtcc caatcgttgc ggctggcgat
       61 ggtctgcgcg acgatattat cggcccaggc ggtgccgccg aagccgaagt tgatgccgac
      121 gcagttcaac tgctcggcag cctgccagac ataggtttcc cgcgggctgg tgacgccatg
      181 accttgcgtg atggagtcgc cgtgaaaaaa tattcgcggc cgtttgtccg gcacggcggg
      241 ctccaagtgg gcgccggcgt cgaccccgat ggcgtacaag atgatctctg aattgcggta
      301 gtagaaaggc agcagcactt gaatctccgc cgcgccggcg agttcccgat cgttgaagat
      361 ggttacttcc tgacgctcga aatgcgtcag ccccgccatg ctggcgacat acttgccgtc
      421 gcggtaaagc gcgaagaaat ttggccggga gcgctcccag cccgcagccg ctgtcgccgg
      481 cggcgtgtcc ggcgcatcga cgaaggtcac gcgaaaaact aatttctttg agtcggtgcg
      541 gaatcgcacg gtcattccgg ctgacgaccg ggcacggcgg gcggtcttct gcggccactg
      601 gtcgcgccaa gccttggtga cgcgcaccgg ctggaaacca tcatcaccct ggggttccca
      661 atcggcgacg ttgagccaat cgaaccgttg atcgcgcgga tcgagccatt gcagcgcatc
      721 gctcatttcc aattttcccc ctcgtacgcg ctgctaaact cccagcgccg gcatgacctt
      781 ggtgccgaac agttcaagcg aatgcatcac ctgttcgtgt ttcaggccgc cgaactggaa
      841 cataccgacc aggctgtcag gcttggccgt ggcaatcatg gcgcgggctt ttttcacgac
      901 agtatcgggg ctgccgatca gcgacgtgcc ccattcgtcc aagcgcttga tcgtcaaaac
      961 gccggggtag taggcatagc gttcggtgaa gcgcgtcaga tcggcttccg caaccgtctc
     1021 gccgcgccaa tagttatcgg tggctaattg ccagaagcgc atgatttccg gaacggcttc
     1081 gcgccaggcc tgctcatcgg tgtcggcgac gtagatatcg cgcaccatga tggcgtcgcc
     1141 gttgccgttg acatggccaa ggccggcgag ctcggcgcgg tatttctcga tggctttggc
     1201 gcaatttgcc agaggctggc gcgacagcgc gagattccaa ccgcgccggg ctaccatcgc
     1261 cgggctatcg ggcgatgtcg cgccataata aatcggcggg tgcggtttct gcagcggctg
     1321 aggccggcag gaaatttctt cgtaacccca tgctttgccc gtgtaactga atgtttcgcc
     1381 ggtccaagcg cgcaggagta tctcgatgcc ttcctcaaag cgcgcctggg cggtttcacg
     1441 gtcgaggcga tgtttcatgt cgtccttggg cacgccgcgg ccgaggccga tgttcagccg
     1501 cccgccggtg agaatatcca acatggcgca ttcctcggcc agccgcatgg gatcgtagag
     1561 cggcaacacg ttgactaaat tgccgaagcg cagccgctcg gtgcggcgcg ccagggcggc
     1621 gatcatcaaa ttgggcgagg ccagcatgcc gtagtcggaa tgatgatgct cggcgaacca
     1681 ggcttcattg aagtggagtt tttcggtgag ctcgatctgg tcgaggagtt cgtcgaacgc
     1741 ctgtttctcg gatgcccacg gccgccgctg attgtaacta aaggtgccaa atcgcattta
     1801 gatttgcctc gtttggagtt aatttctgat cgaacctaac ggatgactct ccgcactgtc
     1861 aatgcgtcag gcagcagcca aactatttcc cggcggcgag ttcctttacc gcccggttga
     1921 gcgggccgaa gtcggcgact tggctgagcg caacctccgt cttcaccccg agcagctcgc
     1981 gctgctcgtt gatcagatcc tggacgaccg cgtcgccgat gcggccatcg aggctcatgg
     2041 ccttcaaata aaaatccaag gtgccggcca gcgtgttacg ctccaatccg gggaattctt
     2101 tcatcagtac cagcagcgct tcctgtttgt gctcgcgggt gtagatcatg ctgcggacac
     2161 tggcgcgcag aaaccgctgc acttgatcgg gtcgttcctt cagttttttc gcgctagtgc
     2221 tgagaccatt ggacggcgtg tcgccgagga gatcgcccag ccagaccaga ttgcgcatgc
     2281 ctttttgctc ggccattacc gtatacggcg gcgttaaaat cgccgcttcg atggcgccgg
     2341 atagcagtgc ctgaaagcgc acactggtat cgcccatggc catcaacgtg acgtctcgca
     2401 aggcttcgag ccctgctggc ttgagcgctt cgcgcgctgc cttgtcggtg gagctggcga
     2461 aaccggagat cgcgatgcgc ttgcccttca agtcttgcac ccgcttgata tccggatgag
     2521 cgtaaagaaa aaaactctgc ttggtgatca aggttgcgat gatcttgagc ggcaatccgg
     2581 acaccgccga gcgaatggtc gaaccgtaga tcgtcgtgta gtcgagctcg cccgagagca
     2641 gcgcgggaat gataataccg ctgcgcatga ctatgaactg gggctccaac ccctcgctcc
     2701 ggtagatgcc gctcttgatg ccgacgaaga acggcgcgaa gtcgaccagc ttgccgggga
     2761 tgccgatgcg aatggtttcc accgccggct gggcggcgtt gccaggctgt gtgactgcga
     2821 ctgctgcgaa tatgaatagg atgagtagaa cgacgcgtgc aatcatcgcg gcgatagtag
     2881 caactttatc tccagatgcg aagaattcca tttgacggcg tggttttgtt tcggctaggt
     2941 ttcagacttc taatttgtga tcgggggaat gaaatatgac tgctgtcaac aagatcgatg
     3001 tgccggccga acattgggat aagctgtttg ccccgagtgc gtgcctggtg atgattacca
     3061 cggttgacga tgccggccga gtcaacgccg cgtcctatgg cacctgcgtc agggtatgcc
     3121 atgatccggt ctacatcgcg ttcacgaccg gctctaccaa agacacttac aataatattt
     3181 tggccaatgg cgaatttgtc gtgaacgttc cgtctcacga gcgcgagatt ttggaaaagg
     3241 tgcgggtcgt cggcctggaa tttccgcccg gagtcaatga gcttgagaag gcagggctca
     3301 cggcgatccc ggccaaagtt gtcaagccgc cgcgcatcgc cgattgccgc agtcactttg
     3361 agtgcaaagt ggagtggacc aagcagtggc tgcatcgatt gatggttgtc ggcaaagtcg
     3421 ttggtgcatc cgtcgacgat ggctgcgtcg acgacaaggg ctacatcctg tgggacaagg
     3481 taaagccggc gcattattgc ggccacgagt acaagaacgg cttcgtcgcg gcgtaccagc
     3541 ccatgtgggt agatatgatt tacaatgaac cgataccgcc gcggccggcg gggaagtaag
     3601 agtttagcca caaagagcac aaaggtcaca aatatagaag agactcgaag tcggattttt
     3661 aaccgcgccc ttcgacaaag ctccgggcga tcgggaatgc aagaagcaaa aaaagatttg
     3721 gacccgtagg ggcgggtttt aaacccgccc tagttcggag gtgacctatg tttgaaagct
     3781 ggcggggcgt ggtcggcgat atcaagccga cacaccgccc tggatcgttg gaagagttta
     3841 taaaattgct tcccgagggc atcggcgtga ttcctctcac cgtcggcatc agctcgggca
     3901 ccgagaagga gtttcacgat gtcctcgccg actaccagca gaaggccgcg cagctggcgg
     3961 aattgggcgt ggacttgatc tccatcggcg gcgcgccgcc gatgatggtg catggctaca
     4021 agggcgaaga aaagatcgtg accgatctcg aagccaagca tggcatcccg gtcaacactt
     4081 cggggcgctc gacggcagcg gcgatgaccg cgctggggat taagaatttc gtcggcgtga
     4141 cttatttccc ggagcgcatg aatcaaatct ttagccgtta tttcaccgag gcaggttttc
     4201 aagtcgcggc catggaaggc atcgacgtgc cgttcaaaga tgtcggcaag ctgtccggtg
     4261 aagagattta caaacacacc aagaaagcat tcctcaaaca cgccggcgcg gacgcgatct
     4321 atatgctggg ttcgggctgg cgactgctca agatcgtccg cttgctcgaa caggatctcc
     4381 aggtgccggt ggtttacgcg ctcggcgcta aactttggga ggtacagaag cggtttcacg
     4441 tgcgtgaacc gatcaaaggc tatggccggc tattggaaga gctgccatag tgcgtgctgc
     4501 gctcggaatt tcatatttcg gacgatttgg aatttggcat ctgtaatttg ttatttcccc
     4561 tggttcagcc gccgcgcgcg gcaaggcgga aggtttgctg gtgctcgctg atccaggttt
     4621 gaatccgctc gctgactttt ggatccggcg gcgccgggtg cttgatattg tcgagggtca
     4681 acctcgggaa gcgcgctttg acgccggagt gggaactggt aaagccttct tcggtaaaaa
     4741 ggctttgccc ttcaactgag gcgagaaagt tgtaaaacag ccgcgccgcc gccgggtggg
     4801 tcgctttttc ggaaataccc acgggatgaa tgctgatgac gatcggattg gcctccgcgg
     4861 accagtccac cggcgctcct tttgagcgcg catcttcgat gcgatgggcg cgcgtgatgg
     4921 caaccggaaa ttcgccggcc accagtagat tcgacgtatt ggtgtaacct tgctgggttt
     4981 gcgggtcctg ggcggccaac gctttcataa aatcgcgccc ggccttgtcg cccaagtaag
     5041 tcaccatgcc gccgtaccac tgaaaagcct cgaccggcag accgatcttt tttcctttcc
     5101 accggggatc caagagatcc ttccagtctt ttggcgcctc ggccggtttg accatccggg
     5161 tattccaggc aatggtcgca acggcgccat atatactgac ccacgtgcca ttggggtcgc
     5221 gatgcctggg cgagaattgc gcggcgtccg gtgacgcata gcgcgccagc gccacatgct
     5281 tggagagctc ggagaactgg atatattccg acgtcaccac gtcccacaaa tattttcccg
     5341 cccgcgcctc gctcctgatc cgttcgacaa atgacccgcc gccgagacgc aggtatttaa
     5401 ccttgacgcc gggaaatttg ttttcaaagt ttttaatgat ttggccgacg atcgctccgc
     5461 cggtgagggt gccgtaccag acgacctcgc cttccttttt cgcttgatcg atggttggca
     5521 tagcttgttg agccccggcc tgattgaccg gatcaagcga aaccaacagc agcgtcaccg
     5581 tcatcaggct gcgaaaaatc gccataagga actccttttg aatggacgga tagcctgtga
     5641 tgagtctggt tatcgtcata gcgccaatgg gagtcgcatg taaatggcta ttttcgaggt
     5701 tcaaccgccg gccgaattgc caacctcgga ttgtcatcct gaataatcga gaataaccat
     5761 tggtcactgt ttctttgcga gttgacagtc attgcgccgt agcgctatgc aggacgaaac
     5821 agcgcaggcg gaggtgatcc tcatgaacca gctggcagcg caagataacg tcgagctcct
     5881 cgatcgcgac atggcgggta agaatctttc cgggtactgg cggctcggca tggaggggtt
     5941 gccggattat ccggtgaccg cggtgaagcc ttgtctatgg aagtggcagg atattcacga
     6001 cagcctggtg cgcgcgggag aggtgatcag cctggagaac agcgaacgcc gcgtcgtgcg
     6061 gctagtcaat ccgggtctcg atcgcaaaca cgcttttgcg acgcacacga tccaagtttc
     6121 atttcaatat gttaaacccg gtgagaacgc gcgggcgcac cgccacacgc cggcggcgct
     6181 gcgcttcgtc gtcgaaggca acggcgcgta tacgacggtc aacggccaac aatgcgtcat
     6241 ggaaccgggc gatctgatcc tcacgcctaa gctcacctgg cacgatcact ccaacgattc
     6301 gaagataccg atgctttggc tcgacggttt ggactttcct ctggtgacgg cgctgcatca
     6361 agtcatgcag gagcgctatc ccgagcgccg ccaggcgatc gagacaaata gcgaatcggg
     6421 aatcggcggc gccagcggtg cggtgagtgg tggccggcca ttagccgaat tcttccacta
     6481 caaatggcgc gacatagaac ccgggttgcg cgccttggtc aaatcggcaa atgcacgaga
     6541 tcgttttgac ggctatctat tcgaatatcg caatccgctc accggcgggc cgacgatgac
     6601 gacaatgcaa tgcgcggtgc agcttctgtc agcgcacgaa gagacggacg cacatcggca
     6661 cacgagcacg gtgatgtatc atgtgtttcg cggcagtggc acgacggagg tcggcgagca
     6721 gcgcttcgac tggcagcagg gcgacacctt tgtggtgccg ctctggcatt cgcaccggca
     6781 tagcaatggg tcatcggacg aggcgattct tttctccatt agcgatgcgc cggcgctcaa
     6841 agcgctcggt ctttatcgcg aagagttctg agcttgatag agttgatcat gcctggcagc
     6901 gcacgcaact ttcggcgcat ggtcgcactg ctattcactg tccaagcgat gggctggctg
     6961 gccgcggcgc agccggcgtt ggcgttggaa gaaattctga tttcctacgc cgggccgagc
     7021 atcacgtttt tgcccgccga ggtggcgc
//
LOCUS       DASQKS010000292         4438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5182666, whole genome shotgun sequence.
ACCESSION   DASQKS010000292 DASQKS010000000
VERSION     DASQKS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5182666"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..370
                     /locus_tag="VE170_07570"
     CDS             149..370
                     /locus_tag="VE170_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HYJ15333.1"
                     /translation="MNWDTISGQWKQMKGKIKEHWGDLTDDELDKIAGKRDQLIGKIQ
                     EKYGLSKDEAERRVSDWTDKIDTTSRTHN"
     gene            475..825
                     /locus_tag="VE170_07575"
     CDS             475..825
                     /locus_tag="VE170_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HYJ15334.1"
                     /translation="MKNHELNANGKNTNGKNKGSYLLLGMGIGAVFSLLFAPRAGQEL
                     RHAIREKTDKSRRALERQSQRLSEVAHSVVDKSKQYIQSGRETVSGAVDASKQAYRNT
                     REGTRNEFNDRPRD"
     gene            866..1294
                     /locus_tag="VE170_07580"
     CDS             866..1294
                     /locus_tag="VE170_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15335.1"
                     /translation="MTYLTVFIGILTVALAAQAVILFFIYRRLTKLTDEVEKSLAHMT
                     DQSKIILNQVSSLMDEINKQAQRYGQVGIEISSRVQQKVNGFLDGVERIGTVTSNGAA
                     VVAREASAALHGLLAALSHLGRRPKRKALPPPPQENMAVH"
     gene            complement(1377..2735)
                     /locus_tag="VE170_07585"
     CDS             complement(1377..2735)
                     /locus_tag="VE170_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ15336.1"
                     /translation="MQGNILIAEDELLPRKNICRVLEDEGYQIHEAADGTEAIEAVDK
                     NDLDLIILDLKMPGADGMTVLKHVRQASPQTLVLIMTAYASIETSVEALRLGAQDYIL
                     KPIVLDGVLKKVRTLMENKRLAWEIQTLRRQLNRDVESTELIGKSPATAEIVELIEKV
                     APTNSTVLISGESGVGKEVVARTIHKQSTRSDKTFLAVNCSAIPETLLESQLFGHVKG
                     AFTGASNYQEGLFQRANGGTIFLDEIGEMPLILQPKILRAIEERAVTPIGAAKPVQVD
                     VRIIAATNRTLKEEVDEGKFRGDLFYRLNVIHLDIPPLRERREDIPLLVEHFIRRQNT
                     ELKTNYQGVESSALKVLMSLPWKGNIRDLNNVLERAMILGNGEWITVKDLPRWEVPEE
                     ESDRSGAHGHDLRRTVRAFEKSHIENVLKETDGDRTQTAELLGLSRSSLYRKLEALGI
                     DS"
     gene            complement(2945..3226)
                     /locus_tag="VE170_07590"
     CDS             complement(2945..3226)
                     /locus_tag="VE170_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15337.1"
                     /translation="MHAILEEAIKTYRMNVSAKGGRDYRLFLDVQTWIMARNDDWLFS
                     FENICEVLGLDPDFVRSGLTRWKELQSDVATVPKAVRYRGSKRALRAVP"
     gene            complement(4144..>4438)
                     /locus_tag="VE170_07595"
     CDS             complement(4144..>4438)
                     /locus_tag="VE170_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15338.1"
                     /translation="ETGGRVALPVFKELMLQVYREKLVGPVPPFPAQMEQNINDYLAG
                     GPVVTEESASTLVSAQSAPLSMHIADRGIESPGHPFNHAELSLRGDSSLNQIR"
BASE COUNT         1109 a         1134 c         1116 g         1079 t
ORIGIN      
        1 gggctcgccg tcggcttaag cgccgccggt attgtcgcca ctataatcct ccggcgcgcg
       61 atccgatagg ctctcttctc cttggcgcgg gcatgccttt tgctcgatta taactcgtgt
      121 cagtaagaaa tttttaggga ggtggattat gaattgggac acgatttcag gacagtggaa
      181 acagatgaaa ggtaaaatca aagaacattg gggagacctc accgatgatg agcttgacaa
      241 aatcgccggc aagcgcgatc aattgatcgg caagattcaa gaaaaatacg gcttgagcaa
      301 ggatgaagcg gagcgccgtg tctccgattg gacggataag atcgacacca cgagtcggac
      361 gcataattaa ataaaaaaat cgagcaagcg aggcaattaa agatcggcga atttataaac
      421 cgttttcagt ccgaatattt cgtctcaagg aatcttaccc cggagggcaa atccatgaag
      481 aaccacgaac taaacgccaa cggaaagaac accaacggaa agaacaaagg aagttaccta
      541 ttgctgggta tgggcatcgg cgctgttttt agccttttgt tcgcgccgcg cgctggtcag
      601 gaattacgac atgctattcg agaaaagacg gataaaagcc gcagagcgct agagcggcaa
      661 agccagaggc ttagcgaggt tgcgcatagc gtggtggata aaagtaaaca gtatattcaa
      721 agcggccggg aaacagtaag cggtgctgtc gacgctagca agcaggcata tagaaacacc
      781 agggaaggga cgcgcaacga atttaacgac cgtccacgtg actagacccc tgatttacta
      841 aaggagagcc accgtggaat cttacttgac ttatctaacg gtatttatcg gcatcttaac
      901 tgtcgctttg gccgctcaag cagtgatttt gttttttatc tatcgtcgac tcaccaagtt
      961 aactgacgaa gtcgaaaagt cactagcgca catgaccgac caaagtaaaa ttattttgaa
     1021 tcaagtttcc tcattgatgg atgaaatcaa caaacaagca cagcgctacg gccaggttgg
     1081 aattgaaatc tcttcacgcg tgcagcaaaa agttaacggg tttctcgacg gagtcgagcg
     1141 tatcgggacg gtgacgtcaa acggtgctgc ggtcgtggcg cgcgaagcca gcgcggcgct
     1201 ccacggcctg cttgccgccc tctcccactt ggggcgccgc cccaaacgga aagccctgcc
     1261 gccgcccccg caagaaaata tggcggtgca ttgaaaaacg aaaaaagctg aacgaataat
     1321 atccagccac tcgcgcttcg cgcggagtgg ctggatgggc tagctaccga caggatttaa
     1381 gagtcaatcc ccagcgcttc gagtttacga tataaagatg agcggctcag gccgagcagt
     1441 tcggcggtct gagtacgatc gccgtcagtt tccttcagaa cattttcgat atgacttttt
     1501 tcaaaggcgc ggaccgtccg cctgagatca tggccatgag cgccgctgcg gtcactttct
     1561 tcctcaggca cttcccaacg aggtagatct ttcaccgtaa tccattcgcc attaccgaga
     1621 atcatggcgc gctcgagcac attgttgaga tcgcggatgt tccctttcca cgggagcgac
     1681 atgagtacct tcaatgcgct gctttccacg ccctggtagt ttgtcttgag ttcggtattc
     1741 tggcgccgaa tgaagtgttc gaccaaaagt ggaatatctt cacgacgttc gcgcaaagga
     1801 ggaatatcca ggtgaatgac gttcaagcga taaaataaat cgccgcgaaa tttaccttcg
     1861 tccacttcct cctttagtgt ccggttggtc gctgcaatga tccgcacatc gacttggacc
     1921 ggcttggcgg cgccgatcgg ggttaccgct ctctcctcaa tggcgcgcaa aattttcggc
     1981 tgcaatatca gcggcatctc gccgatctca tccaaaaaaa tcgtcccgcc gttggcgcgc
     2041 tgaaacaaac cctcctgata attcgaagca ccggtaaatg ctcctttaac atggccgaat
     2101 agttggcttt ccagcagggt ttccgggatc gcgctgcagt tgaccgcgag aaaagtctta
     2161 tcgctacgcg tgctttgctt atgaatggtg cgagccacta cttctttgcc gactccgctc
     2221 tcgccactaa tgagtaccgt cgagttggtg ggtgctactt tttcaatcag ctcgacaatt
     2281 tccgcagtgg cgggactctt tccgattaac tcggtgctct cgacgtcgcg gttgagctgt
     2341 cggcgcaggg tttggatttc ccaagcaagc cgcttattct ccatcagcgt gcggactttc
     2401 ttgagcacgc cgtcaagcac gattggttta aggatataat cctgcgcacc aagccgcagc
     2461 gcctcgaccg aagtttcgat tgatgcgtaa gcggtcatta tcagcaccaa ggtctggggc
     2521 gaggcttgtc tcacatgctt gagcaccgtc ataccatcgg cgcctggcat ctttagatcg
     2581 agaataatta aatccaaatc atttttgtcg accgcttcta ttgcctcggt cccgtcggct
     2641 gcttcgtgaa tttgatagcc ctcatcttcg agcactcggc aaatattttt tcggggcaac
     2701 aattcgtctt ctgcaatcag gatattacct tgcattaccc tctcctgtgt cttttaaggg
     2761 tcatttaggg caatccacgt gccaaaaatg agacgtaatt taattgcgct tttagtagac
     2821 atccgtctca tttataggac atcagatttc gtgccgtccc gtttttggga cataccgctg
     2881 cgggatgtta atcagaagac gattatcaaa tcaagccgat gtagctgccc ctggcaacgt
     2941 aagcttacgg gacggctctt aatgcccgct tcgatccccg gtaacgcaca gccttaggta
     3001 ccgtggcaac atcgctttgc agttctttcc aacgagtcaa tccgctgcgc acaaaatcgg
     3061 gatctaaacc taaaacctcg caaatatttt caaatgaaaa caaccagtcg tcattacggg
     3121 ccataatcca ggtttgtacg tcgagaaaaa gcctatagtc gcgcccgcct ttggctgaaa
     3181 cattcatgcg gtatgtcttg atcgcttcct ctagaatcgc atgcatcaga tgtttctcag
     3241 gttctaaagg tatcgttcgc cgcgagttcc tgagatgttc gtatggggtt aggggatcgg
     3301 gctggaacat ctgggttgag gtctgttcgc tgtgatcaaa tcggctagat tctgtaaatg
     3361 tcataatgca ccgcaaagga ggcaaatttg ataccagctt tttgcattcg aaaaagtcag
     3421 ctgcgctgtc tttgctttac ggcctcgcac agtaatgctg tgcgtcacta ggtttcctga
     3481 ctgttaatgc ttgcggcaaa cagtcaccgc ctttccagca ccgcaaacat cgacattctc
     3541 cattcggcta acactgcgcg aagagtctgg catcgcagtt ctaaaatggt tggttggcga
     3601 tgcgctgcaa tcccgacgag agagggcgat ctgaaaccac tagagggttt ttcgcttttg
     3661 cgagcgcgag ctgatgccgc gattggcgcc gtcaagcccc ggcgatgcgc ccgcgcgcgc
     3721 tgtcgagccg aacatccatt gtcgtaaatc gcggtccatc aaaaggaatc aaaaatgaac
     3781 gctttcacgg cttggcacgt cacgaactat caattaagta atttaactaa gtaatttaac
     3841 aggttctgcg gtttatccgg gattttggac accaagtgac gcgcgtaatc atgccgagtg
     3901 gaagatgacg agaaccggtt cggaatgttg atgggtgcaa ttcggtgaac tcgatccatc
     3961 gcgcctggcg atttccttaa catagtagca agctttatag gtaaacgtat taagaccagc
     4021 taccggcgcc aacgagaata tccccacccg ccgccaccga ccaacaacac cacgagaata
     4081 acgatcaata aagtatccat gactctcacc tttcaatagt ttgtttttgt cctcataacg
     4141 acttcagcga atttgattga gcgatgaatc accgcggagg ctgagctccg catggttaaa
     4201 gggatggccg ggagattcta taccgcgatc cgcaatgtgc atggaaagcg gtgctgattg
     4261 tgcagaaaca agtgtgcttg cggattcttc cgtaaccacc gggccgccgg caagatagtc
     4321 gttgatattc tgttccattt gcgccggaaa cggcggcact ggcccgacga gcttttcacg
     4381 gtacacttgg agcatgagtt ctttaaacac cggcaacgcg accctgccac cagtttct
//
LOCUS       DASQKS010000293         6499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_150634, whole genome shotgun sequence.
ACCESSION   DASQKS010000293 DASQKS010000000
VERSION     DASQKS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150634"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VE170_07600"
     CDS             complement(<1..1388)
                     /locus_tag="VE170_07600"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267448.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HYJ15339.1"
                     /translation="MAILTSEATDLAAGQRPVRAPAGLKIPRYFSRAGVNPYDEVDWE
                     QRTATIANEKGKIVFEQSNVEIPKFWSLMATQVVVSKYFRGPLGTPQRETSVRQMIGR
                     VVRTITGWGQKDGYFATDEDASAFADELTHILLHQKACFNSPVWFNCGIEEKPQCSAC
                     FILSVEDTMDSILDWYRKEGVIFKGGSGSGVNLSKIRSAREQLAGGGTASGPVSFMKA
                     ADASAGVIKSGGKTRRAAKMVVLNVDHPDIEDFVKCKVEEEKKAWALIEAGYDSSLDG
                     PAYGSVFFQNANNSVRVTDEFMQAVQEDREWQTKYVLNGQVCETFNARDLMKMIAEST
                     YFCGDPGMQYDTTINDWHTCSNTARINASNPCSEYMHLDNSACNLASLNLLKFLREDG
                     VFDTESFRRTVDIVILAQDILIDNSSYPTTEITANAHAFRELGLGYANLGALLMTLGL
                     PYDSDGGRAYAAA"
     gene            1611..2072
                     /locus_tag="VE170_07605"
     CDS             1611..2072
                     /locus_tag="VE170_07605"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HYJ15340.1"
                     /translation="MARRDPQIERVGEMLETSLKRLDLSSRLADYGVWTIWNQAVGSA
                     IARNAQPEKIRNGTLIVKVSSPVWMQQLQFMKEMIAAKLNEQLKRDVVKNIFFMVGRI
                     DPPDDARSDQPAATPPRLVDAGFIETIEDPAIRDAFKKLLSSFARRPSKPE"
     gene            complement(2089..2853)
                     /locus_tag="VE170_07610"
     CDS             complement(2089..2853)
                     /locus_tag="VE170_07610"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HYJ15341.1"
                     /translation="MSEDTLDTLIDGKLRLYQGRTGYRFSLDALLLAHFVTVKSGDRI
                     VDLGAGNGAIALLLSHLHPSASLTGLEVQPAMAERAKRSVALNRLDDRVAIIAGDLRE
                     IGALFTSSTFHAVVSNPPFRPVRSGRISPNRERQIARHEITGDLKDFLAAAAYLLCPK
                     GRLALIYSAERAVELLVGLRRAGLEPKRLRWVHSFATDGALLVLVEAVKGGRSGIDVE
                     RPQVIYRQGKEYSAEVAKIVAGIAKASAGVGLSGDD"
     gene            3026..3796
                     /locus_tag="VE170_07615"
     CDS             3026..3796
                     /locus_tag="VE170_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HYJ15342.1"
                     /translation="MISFFGRIGQSTIERIAGMGKMFNFLLTSIMLAFRRPAKLNLIV
                     YHIKTIGVDSLSVVALSGFFTGMVMGFQGYYSLRKFSAESYLGSAAALSLLRELGPVL
                     SAFMVTGRTGSAMAAELGTMRATEQIDALDSMAINPIKFLVSPRIIAGLIAMPLLTAI
                     FDVIGIFGSYLVGVAMLGVSSGSFFAGIENSVVFHDIYSGIVKSISFGLIITWICCYK
                     GFYAPPMATGVSQATTESVVLSFVLILVWDYFLTSVML"
     gene            3802..4548
                     /locus_tag="VE170_07620"
     CDS             3802..4548
                     /locus_tag="VE170_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15343.1"
                     /translation="MIQVVNLHKSFRGQQVLRGVNLEFTTGKITTIVGTSGCGKTVLL
                     KHLNALYLPDAGAVLVNGVDITKLSQEDLYEVRAKFGVLFQGAALLDSLSVFDNVAFP
                     VREKTTMTESDIKKKVDYRLEQVGLAKMGSKLPAELSGGMRKRAGLARALIMDPEIVL
                     FDEPTTGLDPVLATSIHQLIARTQDEFGFTGIIVSHTIPQVFEISDYVAMLAGGVIES
                     IAPPAEFRASANPVVQQFIHGKTEGPIQVL"
     gene            4560..5042
                     /locus_tag="VE170_07625"
     CDS             4560..5042
                     /locus_tag="VE170_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HYJ15344.1"
                     /translation="MNHTRTDIVVGIFVAIGIACLGYLAIRLGKLEVFGAQGYVVFAD
                     FASVAGLKPGDPVEIAGVKVGRVEGMGLVEDKARLTLRINDGVKFQEDVIASVRARGL
                     IGDKFVLVSPGASDKIIPAGGKIRETDSPPDIPDLIGKIVGGDLTSKGERKDEPSKSK
                     "
     gene            5020..5655
                     /locus_tag="VE170_07630"
     CDS             5020..5655
                     /locus_tag="VE170_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15345.1"
                     /translation="MSPLSLNNPAKLLLTLWLAAVMLTASQVRAGVPTEQVKATVDKV
                     FVVLRDPRLKSTAKQSERREQVKQVLFNRFNFAEMAKRSLGSNWTRRTPQEQEEFVRL
                     FTEVLERAYADLIESYSDEKIIYLNEKIDGGYADVSSKVVTAKGEEYSINYRAQLIDN
                     EWRIYDVVAENISLVNNYRSQFSRVIAKSSYEELVRRLKDKNEFAGPPPKK"
     gene            5914..>6499
                     /locus_tag="VE170_07635"
     CDS             5914..>6499
                     /locus_tag="VE170_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15346.1"
                     /translation="MANVIDWHAHHTAPEVAERIGQLTGKKPHIDAYDSPDLAKRVKE
                     MDAAGIDMQLICQGAGIYADQLTAAQALEVMHDSNAVLAERIAPHRDRLGGIIALSLK
                     NIAESINEIDRMAKQGFRAVLMYPHVDGEMLVDTPAMEPLYAKINALNLPIFLHGAGT
                     AQSPSLKKLEDGGAGVAYAVLSDGEVAECCLRMIA"
BASE COUNT         1425 a         1890 c         1779 g         1405 t
ORIGIN      
        1 gccgccgcat aggcgcgccc gccgtcggaa tcgtagggca gacccagggt catcagcaat
       61 gcccccaagt tggcatagcc caagccgagt tcgcgaaagg cgtgggcgtt cgcggtgatc
      121 tcggtggttg gataggacga attgtcgatc aaaatgtcct gagcgagaat cacgatatcc
      181 accgtgcgac ggaacgactc ggtgtcgaaa acgccgtcct cgcgcaggaa cttgagtaaa
      241 ttgagcgatg ccagattaca ggcggagttg tccagatgca tgtactccga gcagggattg
      301 gaggcgttga tgcgcgcagt gttggagcaa gtgtgccagt cgttgatcgt ggtgtcgtac
      361 tgcatgcccg gatcgccgca gaaataagtg ctctcggcga tcattttcat caggtcgcgg
      421 gcattgaaag tctcgcagac ttggccgttg aggacatatt tggtctgcca ctcgcggtct
      481 tcctgcaccg cctgcatgaa ctcgtcggtc acccgcaccg agttgttggc gttttggaag
      541 aacacgctgc cataggccgg gccgtccaag ctggaatcgt agccggcctc gatcagcgcc
      601 caggcttttt tctcttcctc gactttgcat ttaacgaagt cttcgatatc ggggtgatcg
      661 acgttcaaga ccaccatttt agcggcgcgc cgggtcttgc cgccggattt gatcactccg
      721 gccgaggcat cggcggcttt catgaaagac accggaccgg aggcagtgcc gccgccggcg
      781 agctgctccc tggcggaacg gatcttcgac aaattgacac cggaaccgga gccgcctttg
      841 aaaatgacgc cttccttgcg gtaccaatcg agaatcgaat ccatggtgtc ctcgaccgac
      901 aggatgaaac aggccgagca ctggggcttt tcttcgatgc cgcaattgaa ccacaccggg
      961 ctgttaaaac aggccttttg gtgcagcagg atatgcgtca gctcatcggc gaacgcagag
     1021 gcgtcctcgt cggtggcaaa gtagccgtct ttttgtcccc agccggtgat cgtgcgcacc
     1081 acccggccga tcatctggcg cacgctggtt tcccgctgcg gtgtgcccag cggcccgcgg
     1141 aaatatttcg acaccactac ctgggtcgcc attaacgacc aaaacttagg gatctcgacg
     1201 ttactctgtt cgaagactat tttgcccttc tcgttggcga tggtcgcggt ccgctgctcc
     1261 cagtccacct cgtcataagg attaaccccg gcccggctga aataacgcgg aattttcagc
     1321 ccagcgggtg cccgaaccgg cctttgaccg gccgccaaat cagtcgcctc agatgtcaga
     1381 attgccatcg ctccaactct cccttctccc tggtcttgcc catatatggt gtgaaacgtt
     1441 tagatgcgca ctagattttg tagttttagt ggcaaatgcc ctggttgtca atcaaaattt
     1501 tcggcaatgg tatttgtcgt gatttttcgc cacttttgac gaaaaaacta aacttacggc
     1561 agcatcacac catctggcat ggatggcgcc gatctgctag ttaggtgatt atggcgcgac
     1621 gagatccgca gatcgaacgg gtgggcgaga tgctcgaaac ctcgctcaag cgactcgatc
     1681 taagctcgcg cctggcagac tacggggtat ggacgatttg gaatcaagcg gtgggcagcg
     1741 ccatcgcccg taacgcccaa ccggaaaaaa tccgcaacgg taccttgatc gtcaaagtat
     1801 ccagcccggt gtggatgcag cagctccagt tcatgaagga aatgatcgcc gccaagctca
     1861 acgagcaatt gaagcgcgac gtcgttaaaa atattttttt catggtcggc cggatcgacc
     1921 cgccggacga tgcgcgcagt gatcagccgg ccgccacgcc gccacggctg gtcgacgccg
     1981 gcttcatcga aaccatcgaa gatccggcaa tccgcgacgc cttcaagaaa ctcttaagta
     2041 gttttgcccg tcgtccaagc aagcccgaat aaatcaatat aaacggtgct aatcgtctcc
     2101 tgacagccca actccagcgc tcgccttggc gatgccggcg acgatcttgg cgacttcagc
     2161 agaatactcc ttgccctggc gatagatcac ctgcggccgt tcgacgtcga tgccgctgcg
     2221 accgcctttg accgcttcga ccaataccaa aagtgccccg tccgtggcga aggagtgaac
     2281 ccaacgcagc cgtttgggct caagcccggc gcggcgcagg cccaccagca actcaaccgc
     2341 ccgctcggcg gaatagatca atgccagccg ccctttggga cacagcagat aagccgcggc
     2401 ggcgagaaaa tctttcaaat caccggtgat ttcatggcgc gcgatttgtc tctcgcgatt
     2461 gggactgatc cggccgctgc ggaccggacg aaagggtgga ttgctgacga cggcgtggaa
     2521 agtcgatgac gtgaacagtg cgccgatctc gcgcaagtcg ccggcgataa ttgccacccg
     2581 atcgtcgagc cggttgagag ccacgctgcg cttggcccgc tctgccatag ccggttgcac
     2641 ttccaaaccg gtgagcgagg ccgagggatg caaatggctc aacaacagcg cgatggcgcc
     2701 attgccggcg ccaagatcga cgatccgatc gccgctcttg accgtgacaa agtgcgccaa
     2761 taacaacgca tcgagagaaa aacggtaacc cgtgcggccc tgatagagcc gcagcttgcc
     2821 gtcgatgagc gtatccaacg tgtcttcgct catgtttttt tagccattcc agatagccac
     2881 ggcgctattc ttgcgctgat gcgaatgttg cgaaatttcg tcttccagca gcggcgtaat
     2941 tgtcggctca accttgcaat gatggacggt tttctatatt tacatcgaaa acgaaatttt
     3001 cgttttgttt tattgtaaaa ccctgatgat ttcattcttc ggtcgcatcg gacaaagcac
     3061 catcgaacgc atcgccggca tggggaaaat gtttaatttt ctcctgacct cgatcatgtt
     3121 ggcgtttcgc cgtccagcaa agctcaacct gatcgtctac cacattaaaa ccataggcgt
     3181 ggactcgttg tccgtggtcg cgctctcggg attcttcacc ggcatggtga tgggcttcca
     3241 gggttattat agcctgcgca aatttagcgc cgagagctat ctcggttcgg ccgcagccct
     3301 gagcctgttg cgcgagttgg gaccggtgct gtccgccttc atggtcaccg gcagaaccgg
     3361 ttccgccatg gccgccgagt tgggcacgat gcgcgccacc gaacagatcg acgccctcga
     3421 ttccatggcg atcaacccga tcaagttcct ggtttcgccg cgcatcatcg ccggtctgat
     3481 cgccatgcca ttgctgaccg ctatcttcga tgtcattggc atcttcggtt cctacttggt
     3541 cggggtcgct atgctggggg tcagctccgg cagttttttt gccggcatcg aaaacagcgt
     3601 ggtatttcac gatatctaca gcggcattgt caaatccatc tccttcggcc tgatcatcac
     3661 ctggatctgt tgttacaagg gtttttacgc cccgcccatg gccaccggcg tcagccaagc
     3721 gaccaccgag tcggtggtct tatcgtttgt cttgattctg gtctgggact acttcctgac
     3781 ttccgtcatg ctctgagcgc catgatccaa gtcgtcaatc tacacaagag ctttcgcggc
     3841 caacaggtct tgcgcggcgt gaatctagaa ttcaccaccg gcaagattac caccatagtc
     3901 ggtaccagcg gctgcggcaa aaccgtattg ctaaaacatt tgaacgccct ctatctgccc
     3961 gatgccggag cagttctcgt caacggcgtc gacattacca aactgagcca ggaagatctc
     4021 tacgaagtgc gcgcgaaatt cggtgtgctg tttcagggcg ccgccctgtt ggactcgctc
     4081 agtgtcttcg acaatgtcgc ctttcccgtg cgcgaaaaaa ccaccatgac ggaaagcgac
     4141 atcaagaaga aagtcgatta tcgattggaa caggttggcc tggccaagat gggcagcaaa
     4201 ttacccgccg agctgagtgg cggcatgaga aagcgcgccg gtctggcgcg ggcgctgatc
     4261 atggacccgg agattgttct gttcgacgaa ccgactaccg gcctcgaccc ggtcttggcc
     4321 accagcattc atcaactgat cgcccgcacc caagatgagt tcggttttac cggtatcatc
     4381 gtcagccata ccattcccca agtatttgaa atttcggact acgttgctat gctcgcgggc
     4441 ggcgttattg aaagcattgc gcccccggcc gagttccgtg catcggcgaa tcccgtggta
     4501 caacaattca tccatggaaa aaccgaagga ccgatccaag tcctctagga gttgcgccag
     4561 tgaatcatac ccgcaccgat atagtagtcg gcatttttgt cgcgataggc attgcctgcc
     4621 tgggctatct cgccatccgt ctcggcaagc ttgaagtatt tggtgcccag gggtacgtgg
     4681 ttttcgcgga ttttgcgtca gttgccggct taaagcccgg agatcccgtc gagatcgccg
     4741 gcgtaaaagt gggccgggtc gaaggcatgg gcttggtcga agataaggct cgcttgacct
     4801 tgcgaattaa tgacggcgtc aagtttcagg aagatgtcat agcctcagtt cgggcgcgcg
     4861 gcttgatcgg cgataaattt gtcttggtat cgcccggcgc atcggataaa atcatccctg
     4921 ctggcgggaa aatacgcgaa accgactcgc cgccggatat tcccgatttg atcggtaaaa
     4981 tagtcggcgg cgatctgacg tctaaaggtg aaagaaaaga tgagccctct aagtctaaat
     5041 aatccagcga agcttttatt gaccctctgg ttggcggcag tgatgctaac cgcgtcccag
     5101 gtccgcgccg gtgtgccgac ggagcaggtc aaggccaccg tcgataaagt attcgtggtg
     5161 ctcagagatc cccgcttgaa gtcgacggcc aagcaatccg aacggcgcga acaagtaaag
     5221 caggtgttat ttaaccggtt caattttgcc gagatggcca aacgttctct cggctccaac
     5281 tggacccggc gcacgcctca ggagcaggaa gaattcgtgc gcctgtttac cgaggtgctg
     5341 gaacgtgcct atgccgacct gatcgagtcc tacagcgacg aaaaaatcat ttacttgaac
     5401 gaaaaaatcg acggcggcta tgccgacgtc agcagtaaag tcgttaccgc caaaggcgag
     5461 gagtactcga tcaattaccg cgcccaattg atcgacaatg agtggaggat ctacgatgtc
     5521 gtcgccgaga acatcagcct ggtgaataac taccgctcgc aattcagccg ggtgatcgcc
     5581 aaatcctcct acgaagagct ggtgcgccgg ctcaaagaca agaatgaatt cgccggccct
     5641 ccgcccaaaa agtagcccgc gctagagttc gttccctgtc atagcgcccg ccgaggcatg
     5701 agcgcgagtc taatcgctcc gttcattcgg gataatccac cccccgaaac ctgtcattgc
     5761 cagcgccgcc gaaattgctt ctcaggcgcg cttccgccgc ttagaaccaa aattcatcct
     5821 cggtaatggc gcgcacttat caaagcgtgc ttgactgtga taagtcagac gcagtattgc
     5881 caactgatca actaatcagc gaaggagacc gccatggcta acgtaatcga ctggcacgcc
     5941 catcataccg cgccggaagt cgccgagcgc atcggccagc ttaccggtaa gaagccccat
     6001 atcgacgcct atgactcccc cgatctggcc aagcgggtca aggaaatgga cgcggccggc
     6061 atcgacatgc agctcatctg ccagggcgcc ggcatctatg ccgaccagtt gaccgccgca
     6121 caagcactgg aggtcatgca tgattctaac gcggtcttgg ccgaacgcat cgcgccgcac
     6181 cgagaccgcc tgggcggcat catcgcgctg tcactaaaaa atattgccga gtcgataaac
     6241 gaaatcgacc ggatggccaa gcagggcttt cgcgccgtgc tcatgtatcc ccatgtcgat
     6301 ggcgaaatgc tggtcgacac cccggccatg gaaccgctct acgccaagat caacgcgctt
     6361 aacttgccga tctttctcca tggcgccggc acggcccaga gcccgtcgct gaaaaaactt
     6421 gaagacggcg gcgccggcgt cgcttacgcc gtcttgtccg acggcgaagt ggccgaatgt
     6481 tgcctgcgca tgatcgcca
//
LOCUS       DASQKS010000294         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_150645, whole genome shotgun sequence.
ACCESSION   DASQKS010000294 DASQKS010000000
VERSION     DASQKS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150645"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VE170_07640"
     CDS             complement(<1..422)
                     /locus_tag="VE170_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15347.1"
                     /translation="MIQIINLTKHYGKLAAVNKLNLEVAPGEIFGFLGPNGAGKTTTI
                     RCMMGILKPTSGQVLLDGHDIVGDAQNAKAISGFIPDRPFIYEKLSGREFLHFVGKLH
                     RLDAARLSARMAELLERLEMTPWQDELVESYSHGMKQRL"
     gene            complement(505..1905)
                     /locus_tag="VE170_07645"
     CDS             complement(505..1905)
                     /locus_tag="VE170_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HYJ15348.1"
                     /translation="MNFLLIQRQSVALPPLLALRGTEKITEGSEEWWAIFYRGEKIGY
                     ASQSIVPKINGYKLKDFSRLNLNLLGTVQPAETRLEMDANDDWILERFDFALNSNQIG
                     FKSRGAVKGNKLTLEIDSAGHRSSHEITLTQSPYLLAALKPYVVTQQLESGKKFHFST
                     FDPSTLSQQVTTIVIEGREQVRIGNHLEPAIKLRQSFGGISVLSWVDVHGRTLKEESP
                     AGMSMTRQSQLEAKSLPSRSEPLDMIAQASIPLARPIADAAARSALELRLAGVNLANF
                     VIDGGRQKLTGDRLAITREDLSDLSAPAIPVKEPRLQTFLQPTAFMQSDHPSIKTLAA
                     RIVGAEPDALKAAVKLKDWTYRELAKVPTVSIPNALEVLQAKKGDCNEHTVLFNALAR
                     AAGIPAKTAVGVVYLRGAFYYHAWSEVWLGRWVALDSVLNQFPADVTHVKFLEGEVDR
                     QIDILQLIGNLKIEVL"
     gene            2023..2493
                     /locus_tag="VE170_07650"
     CDS             2023..2493
                     /locus_tag="VE170_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HYJ15349.1"
                     /translation="MSNKSIAFAADRMLGRLAKWLRILGYDVIYGRHLSGHGLIRAAR
                     ADGRLILTRDRSLARKQPPPFLFIDSNDYVEQLRQVLRAGDLSPGAGLFSRCLVCNGE
                     LQPRSKESVAELVPPYVFATQERFSWCSRCQRLYWPATHQKKMLEALGKMAVTR"
     gene            2659..2734
                     /locus_tag="VE170_07655"
     tRNA            2659..2734
                     /locus_tag="VE170_07655"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2692..2694,aa:Asn,seq:gtt)
     gene            2848..5073
                     /locus_tag="VE170_07660"
     CDS             2848..5073
                     /locus_tag="VE170_07660"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ15350.1"
                     /translation="MASLKQVVGNPTPRIEGELKVSGQAQYAVDVTLPGMAWGKLLRS
                     PIASGKIKRIDTSKAERLNGVRAVITGADCSGLRIGRRLYDMPILADGEVRFIGEKVA
                     AVAADSEDIAEEAVNLIEVEYEETEPLLDPVEALKPGAKLLHPDVMNYKGLPNPLKEP
                     TNDFVYMTWGKGDPEAGFRQADVIVETTFTTPVVHQSYIEPHSCVVSTAADGSAEFWS
                     CSKVPYGVREQVANALKLPQEKLNFNPVYIGGDFGGKGDFMDLAVVYLLSKKAERPVK
                     MVMDYEEEFTAGNPRHASIIHVKTGVKKDGTLVAHRMSFTFDSGAYGAFKPNAFLNGP
                     HLSAGPYKIPHVFIEEHMVYTNKIPCGHMRSPGDPQGFFANESQLDVIAKKLGMDPIE
                     LRQKNLMHDGDIDPTGEEYSYIKTDETLKKALDDSGYQKPKPKNVGRGIALVQWTPAG
                     GIGTVAISLDAKGVATISSAMADQGAGTYTVLSEIIAEELKIPLSQVKVQQLDTQQGT
                     KDTGVGGSRATRVYGNAGYEAALKAVAAIKQAAAEQMGTSPDQVVLAKGAALHPRMER
                     RISYGDIVKAKGAPISVEATFNDNAKIHAASMCVQVAEVEVDPDTGRVELKKFTSTHN
                     TGTVLNPLMHQGQIEGASMTGIGYTLMEEVIINDGKVATTHFGEYKIPTIKDVPVFKG
                     SVSEAPHGAGPYDSMPIGETGNIPTAAAIANAVADACGARITSLPITAEKVYQALHGS
                     K"
BASE COUNT         1180 a         1500 c         1618 g         1099 t
ORIGIN      
        1 agacgttgct tcatgccgtg cgagtagctt tcgaccaact cgtcctgcca cggtgtcatt
       61 tccaagcgct ccagcagctc cgccatgcgt gcgctcaacc gcgctgcatc gagccggtgg
      121 agtttgccga cgaagtgcaa aaactcccgg ccgctgagct tctcataaat gaacggccgg
      181 tcgggaatga agccggagat cgccttagca ttctgggcgt cgccgactat atcatggccg
      241 tcgaggagga cctgtcccga ggtcggcttg agtataccca tcatgcagcg aatcgttgtg
      301 gttttgccgg cgccgttggg acctagaaag ccgaagatct cgccgggggc gacttccagg
      361 ttcaatttat tcacggcggc gagtttgccg tagtgcttgg tgagattgat gatttgaatc
      421 ataggatctc ggattttcgt ctgcggggcc gcttttgctc tgcgcggcta ctcttcgatt
      481 gtcggattgg caagcggcgc catttcatag gacttctatt ttcaaattgc cgatcagctg
      541 gaggatgtcg atctgccggt cgacctcgcc ttcgagaaat ttcacatggg taacgtcggc
      601 ggggaattgg ttgagcacgg aatcgagggc gacccagcgg ccgagccaga cttcggacca
      661 ggcgtgataa taaaacgcgc cgcgcagata gacgacgccg acagcggttt tcgccggtat
      721 accggcggcg cgggcgaggg cgttgaacag cacggtgtgc tcgttgcagt cgcctttttt
      781 cgcttgcagt acctccaggg cgttgggaat actcaccgtg ggcaccttgg caagttcgcg
      841 ataggtccag tctttgagct tcaccgccgc tttaagggcg tctggttcag cgccgacgat
      901 gcgggcggcg agcgttttga tgctcgggtg atccgactgc ataaacgcgg tcggctgcag
      961 gaaagtttgc aggcgcggct ctttgaccgg aatcgctggg gcgctgaggt cgctaagatc
     1021 ttcacgggtt attgccaggc ggtcgccggt aagcttttgc cgcccgccgt cgatgacgaa
     1081 gttcgccaaa ttgacgccgg cgagcctcaa ttcaagggcc gaacgcgctg cggcgtcggc
     1141 gataggtcgc gccagcggaa tcgaagcttg cgcaatcatg tcgagcggct cggagcgcga
     1201 gggcagactt ttcgcttcca gctggctctg ccgggtcatc gacatgcccg ccggcgattc
     1261 ttcttttaac gtccggccat ggacgtcgac ccaagacagc acggaaatgc cgccaaagct
     1321 ttggcgcagt ttgatcgccg gttcgaggtg gttgccgata cggacctgtt cgcgcccttc
     1381 gatgacgatg gtcgtaactt gctgtgacaa ggtcgacggg tcgaaggtcg agaagtgaaa
     1441 tttcttgccg gactccaact gctgggtgac aacatagggc ttgagcgcag cgagcagata
     1501 gggcgattgg gtcaaagtga tctcgtggct cgaccggtgg ccagcagagt caatttccag
     1561 tgtcagcttg ttgcccttga ccgcgccgcg cgatttgaag ccgatctggt tggaattgag
     1621 ggcgaaatcg aaacgctcca atatccagtc atcattggcg tccatctcca agcgggtctc
     1681 ggccggctgg accgtgccca acagattcaa attgagccgg gaaaaatcct tgagcttgta
     1741 gccattaatt ttcggcacga tcgactggga ggcgtagccg attttttcgc cacgataaaa
     1801 aatggcccac cactcctcgc tgccttcggt gatcttctcc gtgccgcgca acgccaggag
     1861 cggcggcagc gcgacgctct ggcgctggat gaggagaaaa ttcatcacag cccagaaaag
     1921 cgcgatgcca atccatatcg atcgcttaac aatcatataa gagaacgtat atggtaaggc
     1981 cccggcgatt atgaatcaat gtgaatcgag aaatcgctga ctatgagcaa taaatcgatc
     2041 gccttcgccg ctgaccggat gttgggccgc ctcgccaagt ggctgcgcat cctcggttac
     2101 gatgtcatat atggccgcca tctctccggc catggtctga tccgtgcggc gcgcgccgat
     2161 ggccggctga ttttgacccg cgatcgcagc ttggcgcgca aacagccgcc gccgtttctg
     2221 ttcattgaca gcaacgatta tgtcgaacag ctgcgccagg tcttgcgcgc cggcgatttg
     2281 agccccggcg caggcctgtt tagccgttgt ctggtgtgca acggcgaact gcagccgcgc
     2341 tccaaagagt cggtcgcaga gctggtgccg ccctacgtct tcgccaccca ggagcgcttt
     2401 tcttggtgca gccgctgcca acggctgtac tggccggcaa cgcaccagaa aaaaatgctc
     2461 gaagcgctgg gaaaaatggc cgttacgcga taatcacgcc atttcgccga gcgtagattc
     2521 aatccggcat tggatcaacg ggaaatgccg gccctcggca aattccgaaa acaggagccg
     2581 ctcaagcggc ggggttaatt cgataaccat gtagaattcc catcgatcgt atgctaaaaa
     2641 aatgcctgtt cttttgcttc cccggtagct cagttggtag agcaggtgac tgttaatcac
     2701 cgggtcgggg gttcgagtcc ctcccgggga gccagatact ttctccgctt cgagcgatcc
     2761 atgcgaccag gtgacttggc cggccgaagc ggagtccgcc ttccctagat aaaaatttta
     2821 ccggttttta gcagcaggag gttcagcatg gcatcgttga aacaagtcgt cggcaatcca
     2881 accccgagaa tcgaaggcga gctcaaggtc agcggccagg cccagtacgc ggtagacgtc
     2941 actttgcctg gcatggcgtg gggcaagttg ctgcgcagcc cgatcgcgtc gggcaaaata
     3001 aagcgcatcg ataccagcaa ggcggaacga ctcaatggcg tgcgcgcggt tatcacgggg
     3061 gcggattgct cgggcctaag aattggccgc agactctacg acatgccgat cctggccgac
     3121 ggcgaagtgc gtttcatcgg tgaaaaagtc gcggcggtgg cggcggacag cgaagacatt
     3181 gccgaagagg cggtgaacct catcgaagtc gaatacgaag agaccgagcc gttgctcgat
     3241 ccggtggaag cgctcaagcc gggcgcgaaa ctcctccatc ccgatgtcat gaactacaaa
     3301 ggattgccca atccgctcaa ggagccgacc aacgattttg tctacatgac ctggggcaaa
     3361 ggcgatcctg aggccggctt tcgccaggcg gacgtgatcg tcgaaaccac atttaccacg
     3421 ccggtggtgc atcagagcta tatcgagccc cattcctgcg tggtcagcac ggcggccgac
     3481 ggctcggcgg agttctggtc gtgcagcaaa gtgccctacg gcgtgcgcga acaagtcgcc
     3541 aatgcgctca agctgccgca agaaaaactc aacttcaatc ctgtttacat cggcggtgac
     3601 ttcggcggca agggcgattt catggatctt gccgtggttt atcttttgtc taaaaaagcc
     3661 gaacggccgg tcaagatggt gatggactac gaagaggaat tcaccgcggg caatccgcgc
     3721 catgcgtcga tcatccatgt caaaaccggc gtgaaaaaag acggcacctt ggtcgcccat
     3781 cgcatgagct ttaccttcga tagcggcgcc tacggcgcat ttaagcccaa cgccttcctc
     3841 aatggcccgc acctgtctgc tgggccgtac aagattcctc acgtatttat cgaggaacac
     3901 atggtctaca ccaacaaaat tccctgcggc catatgcgct cgcctggcga tccccaggga
     3961 ttcttcgcta atgagagcca gttagacgtg attgccaaaa agctgggcat ggacccgatc
     4021 gagttgcgcc agaaaaactt gatgcacgac ggcgatatcg atccgaccgg cgaggagtat
     4081 agctacataa agaccgacga gacgctcaag aaagcgctgg acgattcggg ctatcaaaaa
     4141 cccaagccaa aaaacgttgg gcgcggtatc gcgctggtgc aatggacccc tgccgggggc
     4201 attggcacgg tggcgatttc gctggatgca aaaggcgtgg cgacgatttc ctccgccatg
     4261 gccgatcaag gcgcgggcac gtacacggtg ttgagtgaaa tcatcgccga ggaactcaag
     4321 attccgttga gccaggtcaa agtgcaacaa ttggacacgc agcaaggcac caaagacacg
     4381 ggtgttggcg gcagccgggc gacgcgagtc tacggcaacg ccggctatga ggcagcactg
     4441 aaggctgttg ccgcgatcaa acaggcggcg gccgagcaga tgggcacctc gccggatcag
     4501 gtggttttgg caaaaggcgc ggctttgcat ccgcgtatgg aacgacgcat tagctacggt
     4561 gacatcgtca aagccaaagg ggcgccaatc tcagtggagg cgacgtttaa cgataatgcc
     4621 aagattcatg cggcatccat gtgtgtccaa gtcgcggaag tcgaagtcga tcccgatacc
     4681 ggtcgtgtgg agctcaagaa atttacctcg acccataata ccgggacggt gcttaatccg
     4741 ctgatgcacc agggacagat cgagggcgca agcatgaccg gcatcggcta cacgctgatg
     4801 gaagaagtga tcatcaacga cggtaaagtc gcgacgaccc actttggcga gtacaaaatt
     4861 cccaccatca aggatgttcc ggttttcaaa ggttcggttt cagaagcgcc gcacggagcg
     4921 ggcccctacg acagcatgcc gatcggcgag accggcaaca ttccgacggc ggcagctatt
     4981 gccaacgcgg tggcggacgc ctgcggcgcg cgtatcacca gcctaccgat tacggcggaa
     5041 aaagtttatc aagcgctgca cgggtcgaag tgacctacgg gtcgtttctg caagcatcga
     5101 tcgcaggaat ttcgagtagt tttaagagca gcagcgttgc cgtccgaaga tgagcgcaac
     5161 catgcatgct cgcgcttatc caggcaaaga aatttctttg ttacgggtcg cgcgcctgcc
     5221 gtccagggtt gtcgcaagcc ggcagatgcg ccaatttctc atggagatcc gccgcgattt
     5281 gcctggggaa gcgtgggcta atcgagctcg ctgccaacta aactgcggcg tagctgagct
     5341 ttgccgtgca gccaatgggc gcgcaggtgg cgcttccatt gaatcaccgc ctctcga
//
LOCUS       DASQKS010000295         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2687286, whole genome shotgun sequence.
ACCESSION   DASQKS010000295 DASQKS010000000
VERSION     DASQKS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687286"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..955)
                     /locus_tag="VE170_07665"
     CDS             complement(8..955)
                     /locus_tag="VE170_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15351.1"
                     /translation="MTALLTLLPAPLPAQEKPGAEKVRIAIATTSMAFLVPFVAKDRG
                     LYLKHGSEVELIVMRPNIAMAALLGGDIDYAELIGSIIRSAARNLPVRAISTSIKAPF
                     FSVIGQNKYKSIKDLKGAVIGLTSIGGTNHVSTRITLRQFGLDIDKDVKILAIGEEKL
                     MVDTFKMGRVDAVVLAPPFSVQLKREGFPVLAQTSDHVVIPFSGLGTTIERIKNNRAQ
                     IKRVLKAEIEALRFIQSNPAGTTEVIRKRFNMDEKLARESYDVVVNSFSRDGRVPLDG
                     VDILLQIEKDAKVIPATVTPQMIVDPSLAEEALKELGVK"
     gene            1179..2198
                     /locus_tag="VE170_07670"
     CDS             1179..2198
                     /locus_tag="VE170_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15352.1"
                     /translation="MGYGIIAWVGLLVSLSVAPLSAQESFYRGKTLRLVVGFSAGGGF
                     DVYSRAISRHIGKHIPGNPAVVVENMTGAGSMVAANHLFHAAKADGLTIGNINGGLFV
                     QQLLGWPGIEFDAAKFEHIGVPVKDKAVCAMTKASGVNSYERWAGSKTPIKLGATGPG
                     SATHNVPLILKEALGLPIQLVSGYKGIADVRLAAEGGELSGVCGWTWDSLKATWTRAL
                     DGGDVSIVLQTVPQAIAELPKVPLAIGMAKSDDAKQLIQAGIHDVADMTYSYVLPPRT
                     GKERADTMRKAFNDTMKDPEFLADTKKSRLGIDPMTGEELQRTIARLFKTPAGVVAKL
                     REVLK"
     gene            2195..>2650
                     /locus_tag="VE170_07675"
     CDS             2195..>2650
                     /locus_tag="VE170_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ15353.1"
                     /translation="MSAPSMVDPIDGPALIVDALKTAGVNFVVSLPDVNLSKLLAAID
                     GQADLPHVTLCREEEGIGICTGAYIGGRTAAIVMQNGGLLNSCNALTTTALNFNIPLL
                     LLIYYAGDIGDEGFATLGSVTEPVLRGLGIRYFLLRKNDEIPATIRGAVT"
BASE COUNT          533 a          724 c          823 g          570 t
ORIGIN      
        1 caagaactta tttcactccc aactctttca acgcttcttc cgccagcgac gggtcgacga
       61 tcatttgggg cgtgacggtg gcgggaatta ctttggcgtc tttttcgatt tgcagcagga
      121 tgtcgacgcc gtcgagggga acccggccgt cgcggctaaa ggaattcacc acgacgtcat
      181 aggactcgcg ggcgagtttt tcgtccatgt tgaaacgttt gcggatgacc tccgtggtgc
      241 cggcggggtt gctttggatg aagcgcagcg cttcgatctc ggccttgagc acgcgtttga
      301 tctgcgcgcg gttattcttg atgcgttcga tggtcgtgcc caggccggaa aatgggatga
      361 cgacgtgatc cgaagtctgg gccaggaccg ggaaaccttc gcgtttgagt tgcacggaaa
      421 aaggcggtgc cagcacgacg gcgtcgaccc ggcccatctt gaaggtgtcg accatcagtt
      481 tctcttcgcc gatggcgagg atcttgacgt ccttgtcgat gtcgaggccg aactggcgca
      541 gggtgatgcg cgtgctgaca tggttggtgc cgccgatgga ggtcaggccg atcaccgcgc
      601 cttttaggtc tttgatgctt ttgtatttgt tctggccgat gacgctgaaa aacggcgcct
      661 tgatgctggt cgaaatcgcc cgcaccggta gattgcgcgc cgccgagcgg atgatcgagc
      721 cgatcagttc ggcgtaatcg atgtcgccgc cgagcagcgc cgccatggcg atgttggggc
      781 gcatgacgat cagctcaact tcggagccat gcttgagata caggccgcga tccttggcga
      841 cgaacggcac gagaaacgcc atcgacgtgg tggcgatggc gatgcggact ttttccgctc
      901 ccggtttttc ctgcgccgga aggggcgcag gtaagagcgt gagtagcgcc gtcaaaaaca
      961 ttcctgctcg agctaaccat tttcctggct gtaacatcgt tgtttcaact ccgtttcgtc
     1021 ttgcgcatta tcgtctgacc tgggcaaata tgtcccaaag gcggtcgtaa ctcaatgttc
     1081 cgccgctacc cgtcgcgatt ggttgacagc tagcagtgct tcgcatagtt tcgccgccat
     1141 attcctaacg aagctaaatc gacaaacgag gggacttcat ggggtacgga ataatcgctt
     1201 gggtcggcct gttagtgagc ttgagtgttg cgccattatc ggcccaggag agtttctatc
     1261 ggggcaaaac tttacggctc gtggtgggat tcagcgccgg cggcggattc gacgtctaca
     1321 gccgcgcgat ctcgcgccat atcggcaaac atatccccgg caaccccgca gtggtggtcg
     1381 aaaacatgac cggggccggc agcatggttg cggccaatca tctgtttcac gcggcgaaag
     1441 cagatggcct caccatcggc aacatcaacg gcggattgtt tgtccaacaa ctgctcggtt
     1501 ggccgggcat cgagttcgac gcggcgaagt ttgaacatat aggcgtgccg gtaaaagaca
     1561 aggccgtgtg cgccatgacc aaagcgagcg gggtcaacag ttacgaaaga tgggcaggat
     1621 cgaagacgcc gattaaactt ggcgccaccg gaccgggctc ggcgacgcat aatgtgccgt
     1681 tgattctgaa agaggcgttg ggcctgccaa ttcaactggt ttcgggttac aagggcatcg
     1741 ccgatgtacg tttggctgcg gagggtggcg aattgtccgg cgtctgcggt tggacttggg
     1801 attcgctcaa ggcgacctgg acgcgagcac tggatggcgg cgatgtttcg atcgttttac
     1861 aaaccgtgcc gcaggcaatt gccgagctgc cgaaagtccc gctggcgatc ggcatggcga
     1921 agtccgacga tgccaagcag ctgattcagg ccggtattca cgatgtcgcc gacatgactt
     1981 attcctacgt gctgccgccg cgcaccggca aggagcgcgc cgataccatg cgcaaagcgt
     2041 ttaacgacac gatgaaagat cccgagttcc tcgccgatac caagaaatct cgactcggca
     2101 tcgatccgat gaccggcgag gaattgcaac gcaccatcgc gcgtctcttc aaaacacccg
     2161 ccggcgtggt ggcaaagcta agagaggtgc tgaaatgagc gcgccgtcga tggtcgatcc
     2221 gattgacggc cccgcgctca tcgtcgacgc cttgaagacc gcaggcgtca attttgtcgt
     2281 gtcgctgccg gacgtcaatc tgtccaaact gttggcggcg atagacgggc aagccgattt
     2341 gccccatgtg acgctgtgcc gtgaggaaga gggcatcggc atctgcaccg gcgcgtacat
     2401 cggcgggcgg accgcagcga tcgttatgca gaacggcggc ttgctcaata gctgtaacgc
     2461 gctgacgacg acggcgctca acttcaatat tccactgctg ctgttgatct attacgccgg
     2521 cgatatcggc gatgaggggt tcgcgactct agggtcggta accgagccgg tactgcgcgg
     2581 cctgggcatc cgctatttct tgctgcgcaa aaatgacgag attccggcga cgatcagagg
     2641 cgccgttaca
//
LOCUS       DASQKS010000296         7173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1411934, whole genome shotgun sequence.
ACCESSION   DASQKS010000296 DASQKS010000000
VERSION     DASQKS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411934"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VE170_07680"
     CDS             <1..281
                     /locus_tag="VE170_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15354.1"
                     /translation="ELLRIWRESGKTVLFITHQINEAIFLADRVIVFGSRPGRVKKMI
                     PINLPRPRDLSIKRDPQFLKYEDELWTLIEEEVKKTMLADQVVQNINK"
     gene            317..2011
                     /locus_tag="VE170_07685"
     CDS             317..2011
                     /locus_tag="VE170_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ15355.1"
                     /translation="MGNRFNSQSLILVGCTLFVLYLAGVPLLMLLYGSIRSAPIGEPG
                     AHFTIANYVKAYVDKEFYLLLLNSVYYAIGTCTLTFLIGTFLAWVSERTNTPFKKLFM
                     VMSLIPFIIPGILSTISWILLLSPKIGLINIVVKEALGLEAAPFNVYSMWGMIWAESI
                     HLYPLVFLLMSAAFRNMDTSLEEAALTAGSSTFQTFRTVTLPLMRPAMVSVLLINFIR
                     GIEAFEVPALIGVPAKISVFTTKIFLAIHQFPSDFGLAGAYAVTLLAISTAGVLIYGK
                     ITRREERYATVTGKGYRPRVIDLGNWKWLTLGISFLVFFLAVILPIFVLLWSSFIPYY
                     GVPSWELAGKMTWSNYHYILNYPLAATAFKNSFYLSVGTATLVMLLTSVIAWITVKTK
                     LPGRAFLDTMCFIPIAMPGIVLGVSLIWVYLTLPIPIYGTIWVLLLAYMTKYIPYGIR
                     AASASMIQINKELEEASFTAGGTWFQTFWKIILPLLMPGFTAGWIYISIIALRELSTS
                     ILLYSYNSTVLSIMAFDLWEGGQYTYVCALGVLMVLLLVTMAMVARKLGAKIGIAE"
     gene            2079..3227
                     /locus_tag="VE170_07690"
     CDS             2079..3227
                     /locus_tag="VE170_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15356.1"
                     /translation="MAETRDMVQIESLEKYFGEDKERVHVLKGVSLNIPEGSLYTFLG
                     PSGCGKTTTLRCVAGLERPDGGKISIGGQTVYASGERVYVPTNKRPIGMVFQSYAIWP
                     HMTVAENVAYPLTIQRKSKDEIKKRVGEVLKIVGLDGLEDRPAPKLSGGQQQRVAFAR
                     ALINEPKVMLLDEPLSNLDAKLRVQMRSEIKALQRRTHITTIFVTHDQAEALAISDQI
                     AVMHGGVLIEVGAPHQLYTRPKRRFTATFLGLTNLINGKVLEVDGDSQPGKMETKKGI
                     LSFIPAPGLKKDQAAVISIRPENIPLHKTKPQGLENVVDGIITEAIFMGDSYQCKVAV
                     GDDLVAVHTHPFISVNVGDTVYLHLDPASCNGLPADDREGVDESMLGD"
     gene            3268..4758
                     /locus_tag="VE170_07695"
     CDS             3268..4758
                     /locus_tag="VE170_07695"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15357.1"
                     /translation="MTALEARKLAEDDMKAGKTPKYNDLRDWLEIVDSMGELKKVDGV
                     DWNLEMGTLAEIFARESKDGPTPAVLFDNVPGYPKGFRTLFAQNASFKRMALNLGLDM
                     SLSNLDLVRAFRQKLVNLEPIKHKIVTKGPILENVDSGDKVDLLKFPVPFMHELDGGR
                     FIGTACLVTTRDPDEGWVNFGAYRGMVHDKGSMGLYISPGKHGSIQRNKYFERGKPCP
                     VVVSVGQDPVLFMVSGNEIDYGVGEFDYAGGLKGAPIEVIEGRSTGLPFAANAEIVIE
                     GWMDPTDRKTEGPFGEWTGYYASSSRPEPVIRVENVYYRNDPILCCARPGRPPSDYSI
                     AKCMVKASLIWEQVEKAGVPDVRGVWCHEAGGGRLLNIISIKQRYPGHARQAMLVASQ
                     VHAGAYLGRYVIVVDEDIDPTNTFDVLWALASRSDPVESIEILRRCWSGPLDPRIHPS
                     KKGFNSRAVIDACRPFEWLKDFPPVAESSPELRDKIYNKYKKIIEG"
     gene            complement(5050..6075)
                     /locus_tag="VE170_07700"
     CDS             complement(5050..6075)
                     /locus_tag="VE170_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYJ15358.1"
                     /translation="MAQAKKRYKVLVLFGSAGTPPANQDFTKELKTEDFAAEANVIEA
                     LKTVGHEVRTLGVWDEASLIIDEIKANPPDVVFNLTEHFNEISAYDRNVAGLLEMMAV
                     PYTGSSPTGLTLCKNKGMAKELLAYHKIRVPNFAIYSPGATIKRPSRLKFPLFIKPAE
                     EEASYGISLDSFVEDDDSFDERIRFIHERMNQPALAEEYIEGREIYVSLLGNDRLRVF
                     PFREVIFNAMPEGQPKFSTFKAKWDDAYRKRRGIQNVFAEPFPNGTDQRIGNICKAVY
                     RALRIRGYGRIDLRVTPAGEIVVLEANPNPNLDRDDEFAQSAMKAGMSYPRLVQRIVK
                     LALASRH"
     gene            complement(6079..7128)
                     /locus_tag="VE170_07705"
     CDS             complement(6079..7128)
                     /locus_tag="VE170_07705"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HYJ15359.1"
                     /translation="MAGSSEKAKSSQPENSSVDLAALDDDALLQMRICDLKLKIPGTE
                     LESRIDKFYAELAARDIAVKPICYLGDEWFCPDGAATIAIPFYLAHPRLKRLEAKMMM
                     EVEGDTEAWCLRLLRHEMGHVVNHAYLLKKDKRWQKLFGPPSLEYSESFRARPYSKRF
                     VRHLDGYYAQSHPEEDFAETFAIWLTPDLDWRQQYRDWKALEKLEYVDKLMQELAGKP
                     PLVFSKSKISDAARLRSRLDVHYRRRRRTYAQEFPDFFDGDLRKLFVDAEAAPDSERA
                     ATFLRRSNKLILNAVSAWTGEPKFTISRLLRGLTERCGELDLRLKSQSAPVEITAYLA
                     TLASHYRLTGKFKDS"
BASE COUNT         1498 a         1942 c         2065 g         1668 t
ORIGIN      
        1 ccgaactcct gcgcatctgg agagagtccg gcaagaccgt gctcttcatt acccatcaga
       61 tcaatgaggc gatctttctg gcggatcggg tgatcgtttt cggctcgcgc cctgggcggg
      121 tcaagaaaat gatcccgatt aatctgccgc ggccgcgcga tttgtcgatc aagcgcgatc
      181 cgcagttcct caagtatgaa gacgagctat ggactctgat cgaagaagaa gtcaaaaaga
      241 ccatgctcgc cgatcaggtc gttcagaaca tcaataaata atgactaatg gtttcgagtt
      301 ctagatcggt ttgcatatgg ggaatcgttt taattcccag tctctcatcc tcgtaggctg
      361 tactcttttc gtcttatatc ttgccggggt tccgcttctg atgctgctct acggcagcat
      421 cagaagcgct cccatcggcg agcccggcgc acatttcact atcgccaatt acgtcaaggc
      481 ctatgtcgac aaagaattct atctattgct gctcaactcg gtttattacg ccattggcac
      541 ctgcacgctg acattcctga tcggtacttt tctcgcttgg gtaagcgagc gcaccaatac
      601 gccgttcaaa aaactcttca tggtgatgtc cttgatcccg tttatcatcc ccgggatctt
      661 gagcacgatt tcgtggatcc tgttattgag tccgaagatc ggtctgatca acatcgtcgt
      721 gaaggaggcg ttgggactcg aggcggcgcc gttcaacgtc tattcgatgt ggggcatgat
      781 ctgggctgag tcgattcatc tctatccatt ggtttttctc ttgatgtcgg cggcgttccg
      841 caacatggac acctcgctgg aagaggcggc gctgaccgcg ggatcgagca cttttcagac
      901 ctttcgcacg gtgactttgc cgctcatgcg gccggcgatg gtgagcgtgc tgctgatcaa
      961 tttcatacgc ggcatcgagg cattcgaggt gccggcgttg atcggcgtgc cggcaaagat
     1021 ttcggtgttc accaccaaga tttttctcgc gatccaccag tttccctcgg acttcggcct
     1081 ggccggcgcg tacgcggtga ccttgttggc gatcagcacc gcgggggtat tgatctatgg
     1141 caagattacg cgcagggaag agcgctacgc gacggtaacc ggcaaaggct accggccgcg
     1201 ggtgatcgat ctcggcaatt ggaagtggct caccctcggc atctcgtttc tagttttctt
     1261 tctggcggtg atcctgccta tctttgtttt gctttggtcg tcgttcattc cgtactacgg
     1321 cgtgccgtcc tgggagctcg ctggcaaaat gacctggagc aactatcact acattctcaa
     1381 ttacccgttg gcggcaacgg cgtttaaaaa cagtttttac ctctcggtgg gaaccgcgac
     1441 tttggtgatg ttgctcacct ccgtgatcgc ttggattacc gtcaagacta agctgccggg
     1501 ccgggctttt ctcgacacta tgtgttttat tccgatcgcc atgcccggaa ttgtgcttgg
     1561 cgttagtctg atttgggtct atcttaccct gccgatcccg atctacggca cgatttgggt
     1621 attgctcctg gcctacatga ccaagtacat tccctatggc attcgcgccg catcggcttc
     1681 gatgatccag atcaataagg aacttgagga ggcgtccttc accgccggcg gcacttggtt
     1741 ccaaaccttt tggaaaatca ttttgccact gctgatgccg ggctttaccg ccggttggat
     1801 ttacatcagc atcatcgccc tgcgcgaact gtcgacctcg atcctgctat attcctacaa
     1861 cagcactgtg ttatcgatca tggcgttcga tttatgggaa ggcggccaat atacctacgt
     1921 ctgcgcgtta ggtgtgttaa tggtcttact gctggttacc atggccatgg tggcgcggaa
     1981 attgggcgcc aagatcggaa tcgccgaata attatttgac ttaatgcatc gacagttccc
     2041 tgcgcctgcg gctccgggaa ccaagggaga gtaatcatat ggctgaaaca agggacatgg
     2101 tgcagatcga atcgctggag aagtacttcg gcgaagataa agaacgggtg catgttctga
     2161 agggtgtgtc gctcaacata cccgaaggat ccctctacac atttttgggc cccagcggct
     2221 gcggcaagac cacgaccttg cgctgtgtcg ccgggctgga aaggccggac ggcgggaaga
     2281 tcagtatcgg cggccagacg gtttacgcct ccggcgagcg cgtctatgtg ccgaccaaca
     2341 agcggcccat cggcatggtg tttcagtcct atgccatctg gccgcatatg accgtggcgg
     2401 aaaatgtcgc ttatccgttg accattcaac gcaagtccaa ggacgagatc aaaaagcggg
     2461 tgggcgaagt tttgaagatc gtcggcctcg acggcttgga agatcgtcct gcgccgaagc
     2521 tctccggcgg tcagcaacag cgtgtcgcct ttgcccgcgc actgatcaac gagcccaagg
     2581 tcatgctttt ggacgagccc ttgagtaacc tggacgccaa gctgcgtgtg cagatgcgtt
     2641 ccgagatcaa agcgctgcag cggcgcactc acatcacgac gatttttgtc acccacgatc
     2701 aggccgaagc gctggcgatc tcggaccaga ttgccgtcat gcatggcggc gtgctgatcg
     2761 aagtcggggc tccccatcag ctctataccc ggcccaagcg gcgctttacc gcgaccttcc
     2821 tcggcttgac gaacttgatc aatggcaagg tgctggaagt cgacggcgat tcccagcccg
     2881 gcaagatgga gaccaagaag ggcattctaa gtttcattcc cgcgcccggc ttaaagaagg
     2941 accaggccgc ggtgatctcg attcgcccgg aaaacattcc cttgcataag accaagccac
     3001 agggtttgga gaacgtcgtt gacggcatca tcacagaggc gatcttcatg ggcgattctt
     3061 accagtgcaa agtcgcggtt ggggatgatt tagtggcggt gcatacccat ccattcatca
     3121 gcgtgaacgt tggcgatact gtttacctcc acctcgatcc ggccagctgt aacggtctgc
     3181 cagctgatga tagagagggt gttgatgaat caatgttagg ggactagaat gccccgcaaa
     3241 gcggaatttg aaaggagtgt gaattaaatg acagcactag aggctagaaa attggccgaa
     3301 gacgatatga aagcggggaa gaccccaaag tacaacgacc tgcgcgattg gttggaaatt
     3361 gtcgacagca tgggcgagct gaaaaaagtc gacggcgttg actggaatct cgaaatgggc
     3421 accctcgctg aaatctttgc ccgcgagtcc aaggatggtc cgaccccggc ggtgcttttc
     3481 gacaatgtcc ccggctatcc gaaaggtttt cgcaccctgt ttgcccagaa cgcctcgttt
     3541 aagagaatgg ctctcaactt gggcttggac atgagcttgt ccaatttgga tttggtgcgc
     3601 gccttccgcc agaagttggt caatctcgaa ccaataaagc ataagatcgt caccaagggt
     3661 ccgatcctgg agaatgtcga ctccggcgac aaggtcgact tgcttaagtt tcccgtgcct
     3721 ttcatgcacg agcttgacgg cgggcgtttt atcggcactg cctgcttggt caccacccgc
     3781 gatcccgatg aagggtgggt caacttcggc gcgtatcgcg gcatggtcca tgacaaaggc
     3841 agcatgggtc tgtacatctc tcccggcaag catggcagca ttcaacgcaa taagtacttt
     3901 gagcgcggca aaccttgccc ggtggttgtc tcggttggcc aggatcccgt gctgttcatg
     3961 gtttccggta acgagatcga ttacggcgtc ggcgagttcg actatgccgg cggtctcaaa
     4021 ggcgcgccca tcgaagtcat cgaaggtcgt tcgaccggat tgccgtttgc cgccaacgcg
     4081 gaaatcgtca tcgagggatg gatggaccca accgaccgca agaccgaagg cccgttcggc
     4141 gagtggacgg gttattacgc cagctccagc cggccggaac cggtgatccg cgtcgagaat
     4201 gtttattatc gcaatgaccc gatcctgtgc tgcgcccggc ccggccgtcc gccgtcggat
     4261 tattccatcg ccaagtgcat ggtgaaagcg tcgttgatct gggaacaggt cgaaaaagcc
     4321 ggcgtgcccg atgtgcgcgg cgtatggtgc cacgaggctg gcggcggccg tttgttgaac
     4381 atcatttcga tcaagcagcg ctatccgggt catgcccgtc aagccatgtt ggtcgcttcg
     4441 caagttcatg ccggggccta cctcggccgc tacgtgatcg tggtcgacga ggatatcgat
     4501 ccgaccaaca ccttcgacgt gctgtgggcc ctggccagcc gctccgatcc ggtcgagtcc
     4561 atcgagatct tgcgccgctg ctggagcgga cctctcgatc cgcgcatcca tccaagcaag
     4621 aaaggcttca actcccgcgc cgtcatcgac gcatgccggc ccttcgagtg gttgaaagac
     4681 tttcccccgg tggccgagtc gtctcccgag ttgcgcgata agatctacaa caagtacaag
     4741 aaaattatcg aaggataact cgaaacactt ctgtgaagac aaaaagggcg gccgtgtcaa
     4801 acggccgccc tttttttgtt tgaatgccag tcgcgatgat tgattgttcc ggcgccctgc
     4861 ctgctaaact gagccgaatt tcacaatagt aagaacgcta agctcaatgc tccgagaatt
     4921 gacaacgaaa tcggtaattc gagtgatctc cctctaaaaa ccggttttca gcgctggcgc
     4981 gtttctgcga agtaaatcct ccaagaagtg atgacgggaa aagtcgacga gtggttgcgc
     5041 taagcgaact cagtgtcgcg aggccaacgc gagtttgaca atacgttgca ccaaccgcgg
     5101 gtagctcatg ccggctttca tcgccgattg ggcgaactca tcgtcacggt cgaggttggg
     5161 attggggttg gcttcaagga cgacgatttc ccccgccggt gtcacgcgca aatcgatgcg
     5221 gccgtagccg cggatgcgca gggcgcgata gaccgctttg cagatgttgc cgatgcgttg
     5281 gtcggtgccg ttggggaagg gctcggcgaa aacgttttga atccctctgc gcttgcgata
     5341 ggcatcgtcc cacttggcct tgaaggtgga gaatttcggt tggccttcgg gcatcgcgtt
     5401 gaaaatcacc tcacggaatg gaaaaactcg aagccggtcg ttgccgagca gactgacata
     5461 gatctcccgc ccctcgatgt attcctcggc caaagccggt tggttcatgc gctcgtgaat
     5521 gaaacgaatg cgttcgtcga atgaatcgtc gtcctcgacg aaagagtcga gcgaaatgcc
     5581 gtaggacgct tcctcttcag cgggtttgat aaacagcgga aatttgagcc ggcttggccg
     5641 cttgatcgtc gcgcccggcg agtaaatggc aaaattgggc acgcgaattt tgtgataggc
     5701 gagcagctcc ttcgccatgc ctttgttttt gcacagtgta agcccggtcg ggctcgaacc
     5761 ggtatagggg acggccatca tttccagtag cccggcgacg ttgcggtcgt aggcggagat
     5821 ctcgttgaaa tgctcggtga gattgaatac gacatccggc gggttggctt tgatctcgtc
     5881 gatgatcagc gaggcttcgt cccaaacacc gagagtgcgc acttcatggc cgacggtttt
     5941 cagcgcctcg atgacattgg cttcggcggc gaaatcttcg gtctttagtt ccttagtgaa
     6001 atcttgattg gccggcggtg tccccgcgct gccgaacagg acgagaactt tgtaacgttt
     6061 cttggcttgc gccatggctt agctgtcctt gaactttccc gtgagccggt aatgcgacgc
     6121 tagagtcgcc agataggcgg tgatttccac gggggccgat tgcgacttca agcgcagatc
     6181 gagctcgccg cagcgttcgg tgaggccgcg cagcaatcgg ctgatggtaa acttgggctc
     6241 gccggtccag gcagagactg cgttaagaat caacttgttc gaccggcgca ggaacgtcgc
     6301 ggcgcgctcg ctatccggcg ccgcctcggc gtcgacgaat agtttgcgca agtcgccgtc
     6361 gaaaaagtcg gggaattcct gggcgtaggt gcggcgccgg cggcggtagt ggacatcaag
     6421 ccgggaacgc aggcgcgccg catcgctgat tttcgattta gaaaacacca gcggcggttt
     6481 gccggcgagt tcttgcatta gcttgtcgac atattccaat ttttccaacg ccttccaatc
     6541 gcgatattgc tggcgccagt cgaggtcggg cgtaagccag atggcaaagg tctcggcgaa
     6601 gtcttcttcc ggatggctct gggcgtagta accgtctaga tggcgcacga agcgcttgct
     6661 gtagggacgg gcacgaaaac tttctgagta ttccaacgac ggcggaccaa agagtttctg
     6721 ccagcgcttg tctttcttga gcaggtaagc gtggttgacc acatggccca tttcgtggcg
     6781 caacagacgc aggcaccatg cctcggtatc gccctccact tccatcatca ttttggcttc
     6841 gagccgcttc aagcgcggat gggcaaggta aaaagggatc gcaatggtcg ccgcgccgtc
     6901 cggacaaaac cactcgtcgc ccaggtagca gataggcttt accgcgatgt ccctggcggc
     6961 gagctcggcg tagaatttgt cgatgcgcga ctcgagctcg gtaccgggaa ttttgagctt
     7021 caaatcgcag atgcgcattt gcaacagcgc gtcatcgtca agcgcggcta ggtctacgct
     7081 ggagttctct ggctgtgagc tcttggcttt ttcggatgat ccggccattg gataacttta
     7141 gcagaccacg gggcaagcgg ccagtaagta ctg
//
LOCUS       DASQKS010000297         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3933722, whole genome shotgun sequence.
ACCESSION   DASQKS010000297 DASQKS010000000
VERSION     DASQKS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933722"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1840)
                     /locus_tag="VE170_07710"
     CDS             complement(224..1840)
                     /locus_tag="VE170_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15360.1"
                     /translation="MKHVNPHRGSRINTSWPALVAILFVLPWSGLAAETPRAGGDLVF
                     AVAETPPSFDGHRETTFAMIHPIAPHYSTLLRFDPHNYPKIVGDVAEKWSASRDGLTV
                     TFKLRKGIKFHDGTALTAQDVKATYDKIIFPAEGVASARKASYAMVDKIEAPDDLTVV
                     FKLKHIAASFLANLASPWNFIYSAEKLKKDPRWYEKNILGSGPFVFGEYVAGSHWTGK
                     KNPNYYMPGRPYLDSYRAIFIRDTAPRVAAVRSGQAMVEFRGFNPAARDDVVKSLGSK
                     AVVQESPWACNLMVVFNNEKKPFSDPRVRRALNLAIDRNEASKALAQISVMKFIGGLY
                     RPGSEFATPAAEIGKLPGFGTDINAARNEAKRLLKEAGVAEGFSFVLKNRNVREPYEV
                     TGVFVIDQWRKIGLNVTHTPQEGGPYFNDFRQGNYDVGMDFMCEFMDEPDLYLVKYLS
                     SDKSPINYARYKDPVVDELFERQSRMLDSKDRLPLLRQIEKRLLGEQVYQFPVLWWQR
                     IVPHNGRLKGWKIGPSHYVNQDLRDVWLGQ"
     gene            2029..2172
                     /locus_tag="VE170_07715"
                     /pseudo
     CDS             2029..2172
                     /locus_tag="VE170_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
     gene            2362..>2997
                     /locus_tag="VE170_07720"
     CDS             2362..>2997
                     /locus_tag="VE170_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYJ15361.1"
                     /translation="MRAEISTLEETLPPLRRRQLAPARMMTGKERRLMNPNRGVIKRC
                     ERYADMAISRFVYPLLGRVWNPYSWQLERRFELAETKLSPAGWPAGLPPLRVLLITDI
                     HAGIFLNPETLRRIIHQLMEQDPDLVVIGGDLVTGHVSEAERILDCLIPLTRARRGAW
                     YCMGNHDYFGGDPEELRKNLLAIGIRTLRNDSTIIRHGAGSLVLGGIDDRVL"
BASE COUNT          622 a          916 c          848 g          611 t
ORIGIN      
        1 gcgcgaaagt cccgctgccg gcatacttga gctcgaccac atcccccggc ggtacgcgca
       61 gcatgaagaa aaccaatagc gcgacaccga acagcgtcgg gatcatcaag aataaacgtt
      121 taaggatata gcggcccatt agaaagactt cgaagatggc gggttggcgg gggcgctatt
      181 gtccatcctc gatcgtccat cctcgaccac gcatgcgtgc gcgctattgg ccgagccaga
      241 catcgcgcaa atcttgattg acataatggc tcgggccgat cttccagccc ttgagccggc
      301 cattgtgcgg cacgatgcgt tgccaccata agaccggaaa ctgatagacc tgctcgccga
      361 gaaggcgctt ctcgatctgg cgcaacagcg gcagccgatc cttgctgtcg agcatgcggc
      421 tctgcctttc gaaaagttca tcgaccaccg ggtctttgta gcgcgcatag ttgatcgggc
      481 tcttgtcgct gctcaggtac ttgactagat acaaatccgg ctcgtccatg aactcacaca
      541 tgaaatccat gccgacgtcg tagttaccct ggcgaaagtc gttgaagtag ggcccgccct
      601 cttgcggcgt gtgggtgacg ttgagtccga tcttgcgcca ctgatcgatg acgaacacgc
      661 cggtgacttc gtagggctcg cgcacgttgc ggttcttgag cacaaaagag aacccctccg
      721 ccacgccggc ttctttgagc aagcgcttgg cttcattacg agcagcattg atatccgtgc
      781 cgaaacccgg caatttgccg atctccgccg ccggcgtcgc gaactccgaa ccgggacgat
      841 agagtccacc gataaatttc atcaccgaga tctgcgccag cgccttggac gcctcgttgc
      901 gatcgatcgc caggttgagc gcccggcgca cccggggatc gctgaacggt ttcttctcgt
      961 tattgaacac gaccatcagg ttgcacgccc aaggactctc ttggacgacg gctttgctgc
     1021 ccagggactt gacgacatcg tcgcgcgccg ccgggttgaa accgcgaaac tcaaccatcg
     1081 cctggccgct gcgcaccgcc gccacccgcg gcgcggtgtc gcgaatgaag atcgcccggt
     1141 agctgtccag atagggccgg ccgggcatat agtaattagg attcttcttg ccggtccagt
     1201 gcgagccggc gacatattct ccgaaaacaa acgggccgga gccaagaata tttttctcgt
     1261 accaacgcgg atcttttttc aacttttcgg cgctgtaaat gaaattccac ggcgatgcca
     1321 ggttggcgag aaaagacgcg gcgatatgct tgagcttgaa caccaccgtc aggtcatcgg
     1381 gcgcctcgat tttatcgacc atggcgtagg acgccttgcg cgcgctggca accccttcgg
     1441 ccggaaaaat aatcttatca taggtcgcct tgacgtcctg ggcggtcagc gccgtgccat
     1501 cgtgaaactt gatgccttta cgcaatttga aagttaccgt caggccgtct cgggacgccg
     1561 accatttctc ggcgacatcg ccgacgatct tgggatagtt gtgcggatcg aagcgcaaca
     1621 gcgtgctgta atgcggcgct atcggatgga tcatggcgaa cgtggtctcg cggtggccgt
     1681 caaaggatgg cggcgtctcg gcgaccgcaa agaccagatc accgccggct cgcggcgttt
     1741 ccgccgccaa tccggaccac ggcaaaacga aaagaattgc gaccagcgcc ggccaacttg
     1801 tgttaattcg cgacccacga tgcggattga cgtgcttcat gagactctcc ttggaacgtt
     1861 cgctgctagc accacggtct gcgcccgaac gtaagggatt cgccggcgta aagtcaagat
     1921 tgttatcttg agcggcttgg cggcgcgcag tcgcataccc tactgtgcgc cagtggcagc
     1981 cgccgcaacc gcctcatcgc cagatggctg ccccagctgg cgaactcggc ggtccgcgcc
     2041 gaagccgaaa aggcgctcgg caagagattc gacatccgcg cctttcacga cgagctggtc
     2101 aaggacggcg ctctgccgat cagcatgctt caagccaaaa tggctcgatg gatcgccacc
     2161 acttcacggt gagcacatct tcgcgaacaa acctcgaccc tttacccaag agatacgctt
     2221 cgaacccacc cgatacatcc ggttgccgtt tgtgaatttt acacggctcg gttccttagc
     2281 atcgctcagg acaacttcgc ggcgaaatct tccgctcaga tgcattcgcc accccagcgg
     2341 gttacaatat gtttttaaac gatgcgcgcc gagattagca ccctggaaga gaccctgccg
     2401 ccgctgcgcc ggcgccaatt ggcgccggca cgcatgatga ccggcaaaga gcgccgtctg
     2461 atgaatccca atcggggcgt gatcaagcgt tgcgagcgct acgccgacat ggcgatttcg
     2521 cgcttcgtct atccgctgct tggcagggtg tggaacccct acagctggca gctcgaacgg
     2581 cgcttcgaac tcgctgaaac caaactttct cccgccggct ggcccgcagg cctgccgccg
     2641 ctacgcgtgc tgctgattac ggatattcac gctggcattt ttctcaatcc ggaaactctt
     2701 cggcgcatca ttcaccagtt gatggaacaa gaccccgatc tggtcgtcat cggcggcgat
     2761 ctcgtcaccg gccatgtcag cgaggccgag cggattcttg actgcttgat cccactgacc
     2821 cgggcgcgtc ggggagcatg gtactgcatg ggcaatcatg attacttcgg cggcgacccc
     2881 gaggagctgc gcaagaattt attggcaatc ggtatccgga cgttgagaaa cgacagcaca
     2941 atcattcgcc atggcgccgg cagtttggtc ttgggcggga tcgacgaccg ggtcctg
//
LOCUS       DASQKS010000298         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1007959, whole genome shotgun sequence.
ACCESSION   DASQKS010000298 DASQKS010000000
VERSION     DASQKS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007959"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VE170_07725"
     CDS             complement(<1..965)
                     /locus_tag="VE170_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ15362.1"
                     /translation="MVVFVGVVIAYLSLSPTLMLFYGSFRSKPLGVPGEFTLAHYISA
                     YSDPLTYQLLFNSFVFAGGSALLATALAATLAWISIRTNAPLRRLFEITAIVPNIFPP
                     VMLAVSWTVLLSPRTGLINRLFMQIFNLSEAPLNLYSLWGMIYVEALITVPLAFLMVS
                     AALYSMDPALEESARVAGSSNMQIAWRITLPIVRPALLASAMLNFVRAIESFDTPAII
                     ALPARIEVFTTKIYREAIGAFPPNQNLAAAYGVSLLLITMFFVYFYRHLTARSERYVT
                     VTGRGYRPMIIDLGKWRYAASALAGLILVLIVVLPSLVMIYVSFIT"
     gene            complement(1155..2165)
                     /locus_tag="VE170_07730"
     CDS             complement(1155..2165)
                     /locus_tag="VE170_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15363.1"
                     /translation="MKNGKTLLLLFLSYFALGAGPLWAQAVNVEAAKKEGKVIVYGAQ
                     VPQAMKPLHSAFEKKYGITAEYWRGSSTQVSERALTEWRAGKPGFDVVEGNRGVQLIM
                     RDDGLFQKYLPPSSEKFPAQFKEKDGMITPWRVLPISILYNTDMVKSGDIPKTFDELA
                     SPKWTRKLTMPDPTRHTTTAQFLWNLSKFKGDKWLDLVRALAKQQPLLVESLAPVTTT
                     IIKGEAPVGITYIKYVKQYKGPVAYALMDKYLTDPNYMSLGAKASHPNAAKLYLEFIC
                     SEEGQKLVAEEGEFVMYPGIFPPIKDAEKVAPNMIFMDNPSEDEFKKLMSNTFREIFF
                     GK"
     gene            complement(2248..>3197)
                     /locus_tag="VE170_07735"
     CDS             complement(2248..>3197)
                     /locus_tag="VE170_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15364.1"
                     /translation="RVSAQGVSLDAAKKEGKVLVYGTIIPQVMKLIEAGFEAKYGVNI
                     EYWRGDATKVVDRVLTEWRAGKPGFDMVIGARGPLSLAKADGVYAKFTPASAANFPAK
                     FRDKDGQLTAWRITPVGILTNTDLVKPGDMPKNWDDLLDPKWQGKITMPDPSRHASTA
                     TFLWNLQKIKGDKWIDFARALAKQRPLLVESYSSVPNAIVRGEASLGISYIQYVPQTK
                     GPIGFAPIDQVFADPSDAAISAKAINPNAARFLIDYLCSPEGQKKVAETHEFVVSPGV
                     YPAIKGADRIVANLTLLDDPSSEQLQKLLSDFRQLFMAK"
BASE COUNT          722 a          908 c          872 g          695 t
ORIGIN      
        1 gtgataaacg acacatagat cataactagc gacggcagaa ccacgattag gacgagaatg
       61 agtccggcca gcgccgacgc cgcataacgc catttgccca ggtcgatgat catcggccgg
      121 tagccgcgcc ccgtgaccgt gacgtaacgc tccgaccgcg cggttaaatg ccgatagaaa
      181 tagacgaaga acatggtaat cagcagcaac gagaccccat acgccgcggc caggttttga
      241 ttcggcggga acgcgccgat cgcctcgcga tagattttcg tcgtaaaaac ttctatgcgc
      301 gccggcagtg caatgatcgc gggggtatcg aaactctcga tggcgcgcac gaaattgagc
      361 atcgccgaag ccagcagggc cgggcgcacg atcggcagcg tgatgcgcca ggcgatctgc
      421 atgttgcttg atcccgccac tcgcgccgat tcttccagcg cagggtccat cgagtaaagc
      481 gccgccgaca ccatcaaaaa tgccagtgga accgttatca acgcctcgac gtaaatcatg
      541 ccccaaagcg aatacagatt gagcggtgct tccgacagat tgaagatctg catgaataat
      601 cggttgatca atccggtacg cgggctcaac agcaccgtcc acgacaccgc cagcatcacc
      661 ggcggaaaga tattcggcac gatggcggtt atttcaaaga gcctgcgcaa cggcgcgttg
      721 gtgcgaatcg agatccacgc caatgttgcc gccaatgcgg tggcaagcaa agcggaaccg
      781 ccggcgaaga cgaacgaatt gaacaacagc tgataggtga gcggatcgct gtacgcgctg
      841 atgtaatgcg ccagggtaaa ctctcccggc acgccgagcg gcttgctgcg aaagctgccg
      901 tagaaaagca tcagcgtcgg cgacaggctc aaataagcga tcaccacacc gacaaagacg
      961 accaccccga gctgcggatt ctgccgaaag gcccgcagcg ggcgatcaaa aagtcggctc
     1021 ggagccgccg aatcagtaag cgccgtgtct ttcactggag tagataaaac aaaaagagga
     1081 ccagccgtgc gcgactatcg cgcgcaggcc agtcctcttt tgctgcaatc gattcagagc
     1141 gatcccgcta ccagctattt gccgaagaaa atttcccgaa aggtgttgct catcagcttt
     1201 ttaaactcat cctcggacgg gttatccata aaaatcatat tcggtgcaac tttttccgcg
     1261 tctttgatcg gcggaaaaat tcccggatac atcacgaact cgccttcctc ggcaaccagt
     1321 ttttggccct cctccgagca tataaactca agatacaact tcgccgcgtt cggatgagac
     1381 gctttggcgc cgagactcat ataattcgga tcggttaaat atttgtccat caaggcatag
     1441 gcaaccggcc ctttgtactg ttttacatat ttgatgtagg taatgcccac cggcgcctcg
     1501 cccttgataa tggtggtggt caccggagcc aaagattcga ccagaagcgg ttgttgtttg
     1561 gccaatgctc tgaccagatc caaccattta tcgcctttga acttgctcag attccacaga
     1621 aactgcgccg tcgtggtgtg ccgggtagga tcgggcatgg tcaatttgcg ggtccatttg
     1681 ggacttgcga gctcgtcgaa ggttttcgga atatcgccgg acttgaccat atccgtgtta
     1741 tacaaaatgc tgattggcag cacccgccac ggtgtgatca tgccgtcttt ttccttgaac
     1801 tgggccggaa atttctccga cgatggtggg aggtattttt ggaatagccc atcgtcgcgc
     1861 atgatcaact gaacaccgcg gttgccttcg acgacatcga agccgggttt acccgcccgc
     1921 cattcggtca gcgcgcgttc cgaaacctgc gtcgacgaac ctcgccaata ttcggccgta
     1981 ataccgtatt ttttttcaaa tgcgctgtgc agcggtttca tcgcttgagg tacctgggct
     2041 ccgtagacaa tgactttgcc ttcctttttg gcagcctcga cgttcaccgc ttgcgcccaa
     2101 agcggtccag cgccgagtgc gaagtagctg agaaacaata gcaaaagtgt cttgccgttt
     2161 ttcatcgatt cctcctattt tgcccgaacc gtgccgaacc tatcgaccgt acccggctaa
     2221 gtcaagcagg gatgtccttg tgtaatccta tttagccatg aacaactggc gaaaatcgct
     2281 aaggagtttc tgcagctgtt cggagctcgg atcatcgagc aacgtcaggt ttgccacgat
     2341 ccgatcggca cctttgatcg ccgggtagac gcccggcgat acaacgaact catgggtctc
     2401 ggcgactttc ttctggcctt ctggcgaaca cagataatcg atcagaaagc gcgccgcgtt
     2461 gggattgatt gccttggcgc tgatcgcggc atcgctcgga tcggcaaaaa cctgatcgat
     2521 gggcgcgaaa ccgatgggac ccttggtctg cgggacgtat tgaatgtagg agatccctag
     2581 ggaagcttcg ccacgaacaa tggcgttggg caccgaagag taggactcga ccagcagcgg
     2641 gcgttgtttc gccaatgcgc gggcaaagtc aatccacttg tcgcctttga ttttctgcag
     2701 gttccataaa aatgtcgcgg tgctggcatg gcgcgacggg tcgggcatgg taattttgcc
     2761 ctgccattta ggatccagca ggtcatccca attcttcggc atgtcgccgg gcttcaccaa
     2821 gtccgtgttg gtcaggatgc ccaccggtgt gatgcgccac gcggtcaact gtccgtcctt
     2881 gtcgcgaaac ttggccggaa agttcgccgc gcttgcgggt gtaaacttgg cgtagacgcc
     2941 gtccgctttg gccagcgaca atgggccgcg cgcgccgatc accatatcga agcccggttt
     3001 accggcgcgc cattcggtca aaacccggtc gaccaccttg gtcgcatcgc cgcgccagta
     3061 ctcgatgttg acgccatact tcgcttcgaa accggcctcg atgagcttca tcacctgggg
     3121 gatgatcgtg ccgtagacca ggactttgcc ttccttctta gccgcgtcga ggctgactcc
     3181 ctgagccgag actcgtg
//
LOCUS       DASQKS010000299         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4777208, whole genome shotgun sequence.
ACCESSION   DASQKS010000299 DASQKS010000000
VERSION     DASQKS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4777208"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VE170_07740"
     CDS             complement(<1..201)
                     /locus_tag="VE170_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15365.1"
                     /translation="MPGAGSQIVANYIYNAAKPDGLTLGSVIPALYFNQLAGKKEAQF
                     DWSKFTFLGSPDRSLNMLYVRAD"
     gene            complement(277..441)
                     /locus_tag="VE170_07745"
     CDS             complement(277..441)
                     /locus_tag="VE170_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15366.1"
                     /translation="MNEIGQYTIGARCQCRVAGFFLALLVIAVPAAAQTPYYQGKTVR
                     LLVGSSADST"
     gene            complement(596..1279)
                     /locus_tag="VE170_07750"
     CDS             complement(596..1279)
                     /locus_tag="VE170_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15367.1"
                     /translation="MDSGLFLFGLLLALVAIYRRAWRRLRSPQATVRDLLRSYHAFER
                     TGLPARESLLRVLTERSGWKKLPPLFLAEIVARFETKENVFRFVSLAESYRFDRAQLP
                     AIAANRDLETAMREVALWLAEFGHRLQKEQSFKQAEFVQKLAVDLQPDQYFTNLPMAA
                     TYFEMGRYDEAAPLFRNGLAQLANCADGAALLGRLEAGANLDEWCARHRDMSAKCAHA
                     LGRRRDAAS"
     gene            complement(1422..1724)
                     /locus_tag="VE170_07755"
     CDS             complement(1422..1724)
                     /locus_tag="VE170_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15368.1"
                     /translation="MGLLRETLLAEVKEAEYFAGKDGKLEIPFTLKGVLPRVEAKADL
                     NRLGRSLREGSLGSVLKNLQKQLPGTSGQRESNARDDRKRTGNEIFRKGLEWLLGR"
     gene            complement(1949..2926)
                     /locus_tag="VE170_07760"
     CDS             complement(1949..2926)
                     /locus_tag="VE170_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15369.1"
                     /translation="MKISWTILGVVMALATPMAVGADDKFEFPLAVSSRVLGFAPAWV
                     ADKKGFFEREGLDVVVTTTRGTSPAMQALAAESVHAALASNDGPIGLVEKGLDLAIIA
                     GGSKTTHMIMGGENITSFEDLRGKVIGSSTLTSGTAFLLRRVLKEKGLEYPKDYSLVN
                     VGGSGPSLIALSTGRVAAGILSVPISFRAAEMGLNLIGKVSDVFPNYLLSSFSVRREW
                     ANHNRPQVVRFLRALLQARKWLGENRDGAAQFLAAELQMKPDQARKGLDYYIENRAWE
                     PDLGVDVEGLKSVVEIYAEQVELKGPLPDPKKYVDMSFLQTALKELGWQ"
     gene            complement(3074..>3762)
                     /locus_tag="VE170_07765"
     CDS             complement(3074..>3762)
                     /locus_tag="VE170_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15370.1"
                     /translation="IEFDGRKFEFIGVPAQDHFVIGLAKATGITNFQGWMTAKKTVKL
                     GGVAPGGATDDVPKVIEATLGAPLRVVSGYKGTGPVRLAFDSGEVDGVCNSWESLKST
                     WRKQLDSGALVPILQATVKSHPELPDLPVALEFARTDEERELIRAVTRIHGPSVRPYF
                     LPPGTAPERVQTLRQAFMNTMKDPEFLAEAKQAKLDTNPGSGEELERNVKEIFKLEPA
                     LIAKLKEILK"
BASE COUNT          769 a         1138 c         1048 g          807 t
ORIGIN      
        1 atcggcgcgc acgtaaagca tgttgagcga acggtccgga ctgcccagga aggtgaattt
       61 cgaccagtcg aattgggctt ccttcttgcc ggctagttga ttgaagtaaa gcgcgggaat
      121 gaccgaaccg agggtcaggc cgtcgggctt ggcggcgttg taaatataat ttgcgacgat
      181 ttgggaaccc gcgcccggca tgttttgcgc aacgaaggtc gggtttcccg gaatatgctt
      241 gccccaatac tgcgcgatca gacgtgcgtc gccatcctaa gtgctgtccg cgctcgagcc
      301 gacgagcagc ctgacggtct tgccttgata gtacggggtc tgtgccgctg ccggcacggc
      361 aataaccaac agcgcaagaa agaacccggc gacccggcac tgacaccgag cgccgatggt
      421 gtattggcca atttcattca taaacaccgc ctttcttagg gtgactttca gtggcccgtt
      481 aacgttagcg atgcacgacg ctttcgattc atcgtggtgg gacattagtg gataaagacg
      541 ctgagtcaac tagctttgaa aataagctgc cagcgcagcg gccttacggc ggcggtcagg
      601 atgcggcatc ccgccggcgg cccagcgcgt gcgcacactt tgccgacatg tcgcggtggc
      661 gcgcgcacca ctcgtcgaga ttcgcgccgg cttccaagcg gccgagcaaa gcggcgccat
      721 cggcgcaatt cgccagttgg gcgagaccgt ttctgaacag cggcgcggcc tcatcgtaac
      781 gccccatttc gaagtaagtg gccgccatcg gcagattggt aaaatattga tcgggctgta
      841 aatcgacagc gagcttttgc acgaactcgg cttgtttaaa gctctgctct ttttgcagcc
      901 ggtggccgaa ctcggccagc cagagagcga cttcgcgcat cgccgtttcc agatcgcgat
      961 tcgcggcgat cgccggcagt tgggcgcggt cgaagcggta gctttcggcg agcgacacga
     1021 agcgaaaaac gttttccttg gtttcgaacc gcgcgacgat ctcggcgaga aagagcggcg
     1081 gcaatttttt ccaaccgctg cgttcggtta agactcgcaa caaagattcc cgcgccggca
     1141 agccggtccg ctcaaaggca tggtaacttc gcagcaggtc tcgtaccgtg gcttgcgggc
     1201 tcctgagccg gcgccatgcc cgtcggtaga tggccaccaa ggccagcagt agcccgaata
     1261 gaaataaacc gctatccatg cagatctccg cttgcagtct gtcagcccga agaatgcccc
     1321 ggcgttacga taaatcaaca ctgacatggc gcgttagcga aatcatggga tcgggtaacc
     1381 gcaggagtcg gagtcgctgg ggctttatct atcatgggga ttcaacggcc aagcagccat
     1441 tccagcccct tacggaaaat ctcgttaccg gtgcgtttcc tgtcgtcacg agcgttactc
     1501 tcgcgttgtc cagaagtccc tggcaactgt ttttgcagat tcttgagcac acttccaagc
     1561 gaaccttcgc gcaagcttcg cccaagccgg ttcaggtcgg cctttgcctc gacgcgcggc
     1621 agcacgccct tcagagtaaa tgggatttcc agcttgccgt ctttaccggc gaagtactcg
     1681 gcctctttga cctcggcgag caaggtttcg cgcaacaggc ccaagcgcag cgagcggctc
     1741 tccttggccg gaaccgctca ttaagaatct ttgaatcgcc gcaccgagcg cctgagaacc
     1801 gtcgagctgg cggagccgga gctttacgca taggctagcg gcggctttca tggcgcgcaa
     1861 ccgatcgaca cctacttatt tcgcgataac caaattactc cgtgatcgag acgtctatat
     1921 tcaatcgctt ccataggagc ggcttcgtct actgccagcc aagttccttg agcgccgttt
     1981 gtaaaaaact catatcgaca tatttcttgg gatcgggcag cggtcccttc agctccactt
     2041 gttcggcgta tatctcgacg accgatttga gcccttcgac atcgacgccg agatcgggct
     2101 cccaggcgcg gttttcaatg taatagtcga gacccttgcg cgcttggtca ggtttcattt
     2161 gcagctcggc ggcgaggaat tgcgccgcgc cgtcgcggtt ttcgcccagc cacttacgcg
     2221 cctgcagtaa ggctcgtagg aaccgcacca cctgcggccg gttgtgattc gcccactcgc
     2281 gccgcacgga aaaactcgac aggagatagt tggggaatac gtcgctcacc ttaccgatga
     2341 gattgagacc catttctgcg gcgcggaaac ttatcggcac actgagaatt cccgcggcga
     2401 cgcggccggt gctcagggcg atcaaagagg gtccgctgcc gcccacgttc accaacgagt
     2461 agtccttggg atattccagc cctttctctt tgagcacgcg gcgcaaaaga aaagccgtgc
     2521 cggaggtcaa tgtcgacgat ccgatcacct tgccgcgcaa atcttcaaag ctcgtgatat
     2581 tttccccgcc cataatcata tgtgtggtct tgctgccgcc ggcgatgatc gccagatcga
     2641 gtcctttttc caccaagccg atcgggccgt cgttggaggc taacgcagcg tgcaccgact
     2701 cggccgccaa cgcctgcatc gccggcgatg tgccgcgcgt cgtggtcacg accacgtcga
     2761 gaccttcgcg ctcgaagaag cctttcttgt cagcgaccca agccggcgcg aaacccagta
     2821 ctctcgacga caccgccagg ggaaactcaa atttgtcgtc ggcgcctacg gccatcggcg
     2881 tcgctaaagc catcacaacg ccaagtatcg tccagctgat tttcatgatt gcatgtcctt
     2941 aaaaaaactc tgattgcgca gcttcattgg taactacatc cggtgatgat cgtccgagat
     3001 cgccgacaac cgaacccacg gtccgtgaga atcgattctc aagcgacact cggcccctgc
     3061 tcaaacaacc ttcctatttg agaatctcct tgagcttggc gatgagcgcc ggttccagtt
     3121 tgaaaatttc tttgacgttg cgctccaact cctcgccgga accggggttg gtgtccaact
     3181 tcgcctgctt ggcctcagcc agaaactccg gatccttcat ggtattcatg aatgcctggc
     3241 gcagggtttg gacgcgctcc ggcgctgttc caggtgggag gaaatagggc cgcaccgaag
     3301 gaccgtgaat ccgggtgacc gctcgtatga gttctcgctc ctcatccgtt ctggcaaatt
     3361 ccagcgccac cggcagatcc ggcaactccg gatgagactt gaccgtcgct tgcaggatcg
     3421 gcactagcgc gccgctgtcc aattgtttgc gccaagtcga cttgagcgac tcccaggaat
     3481 tgcacacgcc gtccacctcg ccgctatcga acgctagtcg caccggcccc gtgcctttgt
     3541 agccggacac cactctgagc ggggcgccta aagtcgcttc gatgaccttg ggaacatcgt
     3601 cggtggcgcc gccgggcgct actccgccaa gttttacggt tttctttgca gtcatccagc
     3661 cctggaaatt ggtgatgccc gtcgctttcg ctaacccgat cacgaaatgg tcctgcgccg
     3721 gcacgccgat aaattcaaac ttgcggccat cgaattcaat gg
//
LOCUS       DASQKS010000300         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5608705, whole genome shotgun sequence.
ACCESSION   DASQKS010000300 DASQKS010000000
VERSION     DASQKS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5608705"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..386)
                     /locus_tag="VE170_07770"
     CDS             complement(114..386)
                     /locus_tag="VE170_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HYJ15371.1"
                     /translation="MIAVKTYYVVFGALLALTLATTGAAFVDLGVRWNTIVGLTIAVV
                     KALLVATYFMHLRHSQRLTLLFAGAGVLWLALLMTFVGADYFTRGW"
     gene            complement(383..1030)
                     /locus_tag="VE170_07775"
     CDS             complement(383..1030)
                     /locus_tag="VE170_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HYJ15372.1"
                     /translation="MPQTPEVLAHQFDDLEQQHDAANLGMWAFLATEILFFGGMFLGY
                     AVYRSLYPEAFAQASNHMDILLGMINTVVLIASSLTVVLAVHAAQHDRRRALVRLLML
                     TILLGLVFLGIKSTEYLHKFNENLVPGVNFAYTEQNPRQAALFFSFYFAMTGMHALHM
                     IIGIAIFVWLVITARRGRYNSIYYTPVEIAGLYWHFVDIIWIFLFPLFYLLGRHL"
     gene            complement(1014..2609)
                     /gene="ctaD"
                     /locus_tag="VE170_07780"
                     /pseudo
     CDS             complement(1014..2609)
                     /gene="ctaD"
                     /locus_tag="VE170_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
     gene            complement(2643..3620)
                     /gene="coxB"
                     /locus_tag="VE170_07785"
     CDS             complement(2643..3620)
                     /gene="coxB"
                     /locus_tag="VE170_07785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYJ15373.1"
                     /translation="MRSSFPLFPEQASTLAGRVDAIYFFLVAVSGFFSLLIFGAIIYF
                     AIKYHHRAGEEHAPGLVNDNLALEIAWTVVPLALTMIMFVWGAKVYFSMSRPPNDSLE
                     IFVVGRQWMWKFQHPDGQREINELHVPLGQPVKLVIASEDVIHSFYVPAFRVKQDAVP
                     GRYTTVWFEATKTGRFHMFCAEYCGTQHSGMIGRIVVLEPVQYAAWLSGDTAVPGAAM
                     AASTLARAEGSVADRGHRLFQQLGCASCHLPDRQGIGPLLNGVFGNPVRLQDGRTTVA
                     DESYIRESILQPQAKITAGFQALMPAFQGRVNEEELMQLLAYIKSLKKG"
     gene            complement(3733..>4271)
                     /locus_tag="VE170_07790"
     CDS             complement(3733..>4271)
                     /locus_tag="VE170_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ15374.1"
                     /translation="LVLEGLGRSLRVLRLAGEDYQVVAVSIDPSETAALAARKKKAIH
                     GAASWHFLTASVPTIEALTQAAGFRYRPSQSAGKESFIHAANTLVLTPEGRISRYFSA
                     IDFPPKELRFALIEASGNRIGSPLDHLLMLCYRYDPAQGRYTLAILNILRVSALITVA
                     AVGGFIAMMLLRERRNTA"
BASE COUNT         1060 a         1174 c         1274 g          763 t
ORIGIN      
        1 cggaagccat tttatcgatt taattgtcac taagacgaga gttgaactaa taccgcgcaa
       61 agcgtatgcc gaaggtcgcc ttcttgaacc ggtccatata gtctttagcc tgctcaccag
      121 ccgcgcgtaa aataatcggc gccgacaaaa gtcatcagca gggcgagcca aagcacgccg
      181 gcgccggcaa agagcagcgt cagccgctgg ctgtggcgca gatgcatgaa ataggtcgcc
      241 accagcagtg ctttgacgac tgcgatagtg agcccgacga tggtgttcca ccggacgcca
      301 agatcgacga aagcggcgcc ggtggtggcc aaagtcaatg ccagcaaagc gccgaaaacg
      361 acgtagtaag tttttaccgc gatcataggt ggcgtccgag caggtaaaac agcggaaaca
      421 aaaaaatcca aatgatatcg acgaagtgcc agtacagacc ggcgatctcc accggcgtgt
      481 aatagatgct gttgtaacgg ccgcggcgcg cggtgatgac cagccatacg aagatcgcga
      541 tgccgataat catgtgcagg gcatgcatcc cggtcatggc gaagtaaaaa gaaaagaata
      601 acgccgcctg ccgcgggttt tgctcggtat atgcgaaatt gactcccggc acgaggttct
      661 cattgaactt gtgcaagtat tcggtggatt tgatgccgag aaataccagc cctaataaaa
      721 tcgtcagcat cagcagccgc accagagcgc ggcgccggtc atgttgcgct gcatgtacgg
      781 cgagcaccac agtcaagctg ctggcgatca gcaccacggt gttgatcatg ccgagaagga
      841 tatccatgtg gttgctggcc tgggcgaacg cttccgggta gagagagcga tagaccgcat
      901 agcctaaaaa catgccgccg aaaaataaaa tctcggtggc aagaaatgcc cacatgccga
      961 gattggcagc gtcgtgctgc tgctcgagat cgtcgaattg gtgcgctagg acttcagggg
     1021 tttgtggcac ggtcttgctc cgatgggtag gtataaacct cactggtcac cactggagta
     1081 acggcgaaat tttccgtggg cggcggcgac gcggtctgcc attccaagcc gctcgcgcca
     1141 aacggattgg cgctcgccgg cgggccgtag cgcatcgacc agatgaaata gatcatcgga
     1201 atgagatagc cgacgccgag gatcgaggcg cccgccgagg acatgacgtt caatacctga
     1261 aactcttcgg ggtaagcgta gtagcgtcgc ggcatgccta aatagcccag gaaaaactgc
     1321 gggaaaaaag tcagattgaa gccgacgaaa atcaccaggg cggcgaactt cgcccagcct
     1381 tccggatata gccggccgga aatttttggc caccaaaagt gcagtccgcc gagatagccc
     1441 atgatggtgc cgccgaccat gatgtagtga aagtgcgcga cgatgaaata ggtgtcgtgg
     1501 acgtgcacgt cgacggccag ggtcgcgaga aaaaggccgg tcaagccgcc gatggtgaac
     1561 aggccgataa agccgagggc gtagagcaga ggggtctgcc aagacaccga acctttgtag
     1621 agagtagccg tccagttaaa aaccttgatc gccgagggga tcgccacgaa catgctcaaa
     1681 aaggaaaaca ccatgccggc atacactgat tggccgctga cgaacatatg gtggccccag
     1741 acgaaaaagc ccagcactgc gatggcgatg ctggagaaag cgacgaacgg ataactgaag
     1801 atgcgtttgc gcgaaaaggt cgtaatcaat tcgctgatca ctcccatggc ggggagaatc
     1861 atgatgtaaa ccgccggatg ggaataaaac caaaacagat gttgaaatag aatcggatcg
     1921 ccgcccagag cgggattgaa aatccccaag cccagcaccc gctcgagggc caacaacacg
     1981 atggtgatcg ccagcaccgg ggtgccgagt aatatgatga tgctggtcgc ataatgcgac
     2041 cagataaaca gcggcaaacg gaaccaggtc agcccggggg cgcgcatggt gtggatagtc
     2101 acgacgaaat tgagcccggt catgatcgag gaaaagccga cgatgaaggc gccgagggcg
     2161 gtagcgatga catagctgtt gctgtagacc gtgctgtagg gcgtttagaa cgtccagccg
     2221 gtatcgacgc cgccggcgag catggcgccc agcaggaaga gcccgccggt gacaaacacg
     2281 taccagctcg ccagattcaa ccgggggaag gcgagatcgc gcgcacccaa catcatcggc
     2341 accaggaaat ttcccaagac cgccggaatc gccgggatca aaaacaaaaa aatcatgacg
     2401 atgccgtgca tggtgaacag cttgttgtaa gtctcggcct gcaccagatc gccctgtggg
     2461 gtcaagagct cgatgcgaaa gatcaccgcc gccgcgccgc cgatgaaaaa aaacagcgtc
     2521 accgcgccga gatacaaaag cgcgatgcgc ttgtgatctt tggtcagcaa ccacgacttt
     2581 acgccgtagt tgacgttgag gtaattcatc tcagacaaac tcgttcaaat cctttaaccc
     2641 agctagcctt ttttgaggga cttgatgtac gccaacagct gcatcagctc ttcttcgttt
     2701 accctgccct gaaacgccgg cattaacgct tggaatcccg cggtgatttt cgcttgcggt
     2761 tgaaggatcg actcacggat gtagctttcg tccgccaccg tggtgcggcc gtcttgcaaa
     2821 cgcaccgggt tgccgaagac cccgttcaac aacggcccga tgccttgacg gtctggcagg
     2881 tggcagctgg cgcaaccgag ttgttgaaat aggcggtggc cacggtcggc caccgatccc
     2941 tcagcccgcg ccagggtcga cgccgccatc gcggcaccgg gaacggcggt atcgccgctc
     3001 agccaagcgg cgtattgcac cggttcgagc accacgatgc ggccgatcat gcccgagtgt
     3061 tgcgtgccgc aatattcggc gcagaacata tgaaatcggc cggtcttggt ggcctcgaac
     3121 cagacggtgg tgtagcggcc cggcaccgcg tcttgcttga cccgaaacgc cggcacgtaa
     3181 aaactgtgaa tcacatcttc gctggcgatg accagcttca ccggctggcc gagcggcacg
     3241 tgaagctcgt tgatctcacg ctggccgtcc gggtgctgaa acttccacat ccactggcgg
     3301 ccgacgacga atatttccag cgaatcattg ggcgggcgcg acattgaaaa gtaaaccttc
     3361 gcgccccaga caaacatgat catggtcaat gccagcggga ccaccgtcca ggcgatctcg
     3421 agggcgagat tgtcgttgac caggccgggg gcgtgttctt cgccggcccg gtgatgatac
     3481 ttaatcgcaa agtaaatgat ggcgccgaaa atgagcagcg aaaaaaaccc gctgaccgct
     3541 acgagaaaga aataaatcgc atcgacgcgg ccggctaaag tagatgcctg ttcgggaaat
     3601 agagggaagc tggaccgcat aactaattca aaacacgttc aagccagtca ttcgacggct
     3661 tcgttccgtt caagcggttg aagccgtcgc ggaggtcgcg acaatttgaa acctgcgcag
     3721 cgcttgaacc gatcaagcag tgttccgccg ctcgcgcagt aacatcatcg cgataaatcc
     3781 gccgacggcg gccaccgtga tcagcgccga cacgcgcagg atgtttagga tcgccagcgt
     3841 atagcggccc tgggcgggat cgtagcgata acagagcatg agcagatggt cgaggggcga
     3901 gccgatgcgg ttgcccgagg cttcgatgag cgcgaagcgc aactctttag gcggaaagtc
     3961 gatggcagaa aagtagcgcg aaattcttcc ctccggcgtg agcaccagag tattggctgc
     4021 atggatgaaa ctttccttgc cggcgctttg gctcgggcgg tagcgaaagc ctgcggcctg
     4081 ggtcagcgct tcgatggttg ggaccgaggc ggtgaggaaa tgccagctcg ccgcgccgtg
     4141 gatggctttt ttcttgcgcg ccgccagcgc cgcggtttcg cttggatcga tgctcaccgc
     4201 aactacttgg taatcctcgc cggccaagcg caacacgcgc aagctgcgcc cgagtccctc
     4261 caagaccagc g
//
LOCUS       DASQKS010000301        10595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4778528, whole genome shotgun sequence.
ACCESSION   DASQKS010000301 DASQKS010000000
VERSION     DASQKS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4778528"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1138)
                     /locus_tag="VE170_07795"
     CDS             complement(206..1138)
                     /locus_tag="VE170_07795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYJ15375.1"
                     /translation="MQYGLHLPASSATVQPAELVRFAQRAEALNFSLLTVADHVIVPT
                     HISVPYPYTVDGKYPGTGYHLETLTTMSYLAGATQRIRFATSVMILPYRNPIVTAKML
                     ASLDVLSGGRVIVGVGVGWMKEEFENLQTPNYEARGRVTDEYIRAFRELWTSDRPSFK
                     GEFVNFGDLIFLPKPVQKTIPIWIGGHSKAALKRAGQLGDGWHPIGGVPTIPLEPEDV
                     KKDLAAMNEYAVKAGRDPKTIHVALKGSLFDKEKKIADRRRRFMGVAEEIAADIREYA
                     AAGVNTMIFDVRRPSSAETLERMEWMAQEVFPQV"
     gene            complement(1316..1882)
                     /locus_tag="VE170_07800"
     CDS             complement(1316..1882)
                     /locus_tag="VE170_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HYJ15376.1"
                     /translation="MAQAIRPMPEELPRELWRGYQPASPAWQGNVKEIYITPASAQAM
                     VAVSKVRAFADRGLEGDRFYRESWSAVNRSDKAVSLIEDEVLALAGREIAVEAIGILT
                     RRNIVTRGVPLIDLLGREFTIGNVRLRGVRLFEPCGHLVAVTKIPAIFKALEHRSGLK
                     AAVLSDGEIQIGDSVVLESAGAAVAAAR"
     gene            complement(1962..2444)
                     /locus_tag="VE170_07805"
     CDS             complement(1962..2444)
                     /locus_tag="VE170_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15377.1"
                     /translation="MQPAKKLAPTEKPHTQKFSLRTPYMKQGRITQLVAETENMWIHT
                     KINYEGGENEIHCHLDEDHSFIVLEGQMSVFDEKGGEIKVEKHQGVMIPKGAYYRYLN
                     TGSENLVVLRIGAGIKGKSQGGEEMRVRPDGKPLIASSEENKTVPPIEDTRFYADAAG
                     "
     gene            complement(2470..3531)
                     /locus_tag="VE170_07810"
     CDS             complement(2470..3531)
                     /locus_tag="VE170_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15378.1"
                     /translation="MTTATGVKRTAEQVLGQDKIVSSDSHIMEPEDLWLKNLTPSLAA
                     KFPKFPPRNTAGEKPGGWDPKARLDEMAVDGVSAEVLYATHGLRLYALEDAPLQEACF
                     EIANDWMMEYCKAAPGRLFGIPMISMYNIPNAIKELERCKKNGMIGCMIWQVPPDHLP
                     FTSDYYNPFWEAAGELDMPVNVHILTGFNYSRFDRKGLDTYRMTVNQKLTDAANTLFD
                     FVFGGPLAKFPKLKLVYVENEVGWIPFYCHEWDKYYVRHSPKAPLAYMKKLPGEYVKD
                     QVFATFFSDPAGGRMMDWFGEDNFMWSSDYPHAASTWPHSRQVITDELGHLPKNVIDK
                     VVRQNVLDLYHIKVDGINN"
     gene            3662..4495
                     /locus_tag="VE170_07815"
     CDS             3662..4495
                     /locus_tag="VE170_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ15379.1"
                     /translation="MRSLKGLFSHCLAVLLIPAALFGAEAQNAFNEGVKHYKAAKFKE
                     AVAAFNRAIKAAPKSAEAYNNRGLAYAKLGQPENALKDYDEALKLNPELTDAQFNRGN
                     VLFKQKQYERAVKDFDEVIKQDAKAPRVFVQRGLAFLQLKQFDKAVQDFNQAQKLDPK
                     DAEIYYNRGLAHRRLGKNELAVQDYSEAIKLDPKNGENYNNRANAYSLLGKYDEAVKD
                     YDEAIKLNDKDARIYANRGVIKQRLKKDDEAQKDFQRAVELDPGLKDKLASFIAPAKG
                     K"
     gene            4499..5554
                     /locus_tag="VE170_07820"
     CDS             4499..5554
                     /locus_tag="VE170_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ15380.1"
                     /translation="MARALVIIGYLLGLTAGAVPAWAGLADDPLKTALELHESGKVKE
                     AVTEYDKVIKTNPKLAEAYFNRGNAHFDLGQNQQAAKDYSEAIRLNPKDAEAYFNRGN
                     TYRRLKQDAAALNDYSSAIKLNPDDAKSYTNRATIHFAARRYEAALADQNQALRIDAN
                     DAQYYFNRGNSYLALGKKEEAIADYNAALSRDPKLADAYYNRGIAHADSNKQEEAVGD
                     FDLAIKLNPKNPEAFYNRGLANGKLGKHDAAIDDYGEAIRLNPKDAEAPFNRGLAYMR
                     VGKHDFAARDFTEVIGLEPKHADAYVYRGLIRVYQGRENDADADFQRAYRLNPALKKK
                     LEPVITEAKAKAKNIAK"
     gene            5570..5989
                     /locus_tag="VE170_07825"
     CDS             5570..5989
                     /locus_tag="VE170_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HYJ15381.1"
                     /translation="MAESNFFGGLTGGLLIGLAAALLLLFNGRIAGISGILGGLIDAN
                     PISERLWRGAFIIGLVCGAAVYSWIHGGLPLQLQVRSSGLLLAGLLVGFGTRLGSGCT
                     SGHGVCGLARSSPRSLVATATFIACGMLTVYLTRHVF"
     gene            5989..6405
                     /locus_tag="VE170_07830"
     CDS             5989..6405
                     /locus_tag="VE170_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HYJ15382.1"
                     /translation="MLPLISSFALGVLFAFGLAISEMIDPARVIGFLDVAGRWDPTLL
                     GVMGGALAVTLPLFPWIQQRRKSLLGEPFHLPSQARIDARLIIGAALFGIGWGLAGLC
                     PGPAIAGLASRSGGVFLFVLAMIAGQWLAARSTRAN"
     gene            6527..8116
                     /locus_tag="VE170_07835"
     CDS             6527..8116
                     /locus_tag="VE170_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ15383.1"
                     /translation="MGETRVDLQHLLEDLRDAYPGSIEETILTEIMANSLDCGATTIQ
                     LITDPGARTLTVTDNGSGMQRRDLARFHNIASSTKSRGEGIGFAGVGIKLGLLVCDEV
                     FTETRRGKTHIASSWHLATRNRAPWKWVAPLKLIAERGTAVRLQVENPLSPLLDPGFL
                     AVTLRRHFQPLLDPSFDEFLRGHYPSGVAIEVDGRRLDKQRWSAPRAAPLEIRLARKR
                     KAAASGYLIRADHPLPEERQGLAISTYGKVIRRGWDWLGVTPAAADCVGGIIEVPELA
                     ACLTLNKGDFIRSGARGATYLGFRKAIQEAVGRQLALWGDGRSGAEQAPPREVRPLQR
                     DLVQVLEDLALDFPLLGSLVEHRAGGQKRLPLGIRGAGSDGHGFVAASLTAAAESEPT
                     ENPRAPAAASTDSSPATASAVVHEANDGYGTDGSSIQLPSKSIVRRPAHYGLDIQFAD
                     RPDDIELGRLTESTVWVNRAHPAYRRAQASHSFGYHIALAVAMALAPLAVEAPHQHGF
                     VSAFLSRWGEAIDKPKARSRH"
     gene            complement(8304..9332)
                     /locus_tag="VE170_07840"
     CDS             complement(8304..9332)
                     /locus_tag="VE170_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15384.1"
                     /translation="MFCNHARAIVLLIFGWVHGAGAHAAEISASDLVDRAKAEGEVVW
                     YSTLPLPETQAWAALFTKKYGIKVSLFRAGATAMTQKLQTEIPAGRWGFDVVMNRASN
                     LELYRRKNWLQKMNYPERNRFLDGFMDPDDYWAAYYIQFWSVAYNTNMLKPNDLPKSY
                     EDLAHPKWKDKLTMDEQDVDLYATWLKLMGRDKGREWFKKLVANGLRPRRGHTMEAQL
                     VSAGEFALGISAYASVIENIRAKGAPIDWVAFEPVPVNVLAWGIGAKPPHPNAARLFL
                     YWGLSVEGLKAMNEISDRVPARKDVPIKNKRLERMMQSKLAPVDMSIADRYAEYNQEF
                     LSLLGLKP"
     gene            complement(9350..10507)
                     /locus_tag="VE170_07845"
     CDS             complement(9350..10507)
                     /locus_tag="VE170_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15385.1"
                     /translation="MKSAYGLKIPEPDGELTRVGPGTPCGELMRRYWQPVCLSADLKD
                     LPKPVKILGEELIAFRDGQGRAGLLFFRCSHRGASLEYGRVEPRGLRCCYHGWLYDVE
                     GTVVEMPLEPAPNPFLPHIQQPAYPVREFGGLLFAYMGPLERQPEFPLYDIWQTAGGT
                     LKARMGPRIGGPVNCNWLQAEENLMDALHTFWLHTLHSGPQFPSQAYGATPDDLKYEE
                     TAMGMRFILTRKLANGKWWDLIWEMLMPLNVHLVYTDAPRTERVRAVTYCVPVDDTHQ
                     LGASVRWLADNETESLSGREQLAPGGRKDSSFEYTQRHPDDKEAVEGQGPIALHGLEH
                     LVSSDKGVMLFRKLLRAAIGEVRAGRDPKGILRDALSARRVGTSAGSVVRD"
BASE COUNT         2156 a         3204 c         3047 g         2188 t
ORIGIN      
        1 aattctcctg ggcgccaaat tttttttggc cgcggggcat tttctcgccg gtgaaactct
       61 cgctgtgcgc gaccgcctcc ttgtcacgat agctgcaaaa gattatcaac ccaactttga
      121 agctcgcttc atggcaataa ctcaaaacgg caagtggaag cccaaaagca tttgatcgcc
      181 ggggaataga accgaacacc ctcgcctaaa cctgtggaaa aacttcctgc gccatccact
      241 ccatccgttc cagggtctcc gccgagcttg gccggcgcac gtcaaagatc atcgtgttga
      301 caccggcggc ggcgtattcg cgaatatcgg cggcgatctc ttccgctaca cccatgaagc
      361 ggcgccggcg gtcggcgatt ttcttctctt tatcgaagag tgagccttta agcgcgacat
      421 gaatggtttt cggatcgcgg ccggctttga ccgcatactc attcatggcc gccaaatctt
      481 ttttgacatc ttccggttct agcggaatcg tcggcacgcc gccgatcgga tgccagccgt
      541 cgcccaactg gccggcgcgc ttgagtgccg ccttgctgtg gccgccgatc cagatcggaa
      601 tggttttttg caccggtttg ggtaaaaaga tcaagtcgcc gaagttgacg aactcgcctt
      661 tgaagctcgg ccggtcgctg gtccataatt cgcgaaaggc gcggatatat tcatcggtga
      721 cccggccgcg cgcttcgtaa ttgggcgttt gcagattttc gaactcttcc ttcatccaac
      781 cgacgccgac gccgacgatc acccggccgc cggaaagcac gtccaagctc gccaacatct
      841 tggcggtcac gatcggatta cgatagggca gtatcatcac gctggtggcg aagcgaatgc
      901 gctgggtcgc gccagcgaga taactcatgg tcgtcaaagt ctcgagatga taaccggtgc
      961 cgggatattt gccgtccacg gtgtaaggat acggcacgga aatatgggtc ggcacgatca
     1021 catgatcggc aaccgtgagc agggaaaaat tcaaggcttc ggcgcgttgg gcaaaacgca
     1081 ccagttcggc cggttgcact gtcgcgctcg atgccgggag atgtaaaccg tactgcatga
     1141 aatcttacct cgttgtcgtg aaaagtttgc ttggattgca caagtcgccg gatggttcac
     1201 ttcagagcgg cacggtacgc cgggaaagaa tcagaaaatc caaattcgga gcgccttgtg
     1261 tcacttcgat gaattcgccg atcggaaggc aacgccggcc gcgatccacg ctggattaac
     1321 gcgccgctgc gactgccgcg ccggccgact ctagcacaac gctgtcgccg atttgaatct
     1381 cgccgtcgct cagcacggcg gctttgagac cgctgcgatg ttccagcgct ttgaagatcg
     1441 ccgggatttt tgtcacggcg accaaatggc cgcacggctc gaacaagcgg acgccgcgca
     1501 atcgcacgtt gccgatggtg aactcgcggc cgagcaggtc gatgagcggc acgccgcggg
     1561 tgacgatgtt gcgccgggta agaataccga tcgcctcgac agcgatttcc cggccggcca
     1621 gcgccaagac ttcgtcctcg atcaagctca ccgctttgtc cgagcgattc acggcactcc
     1681 aggattcgcg atagaatcgg tcgccttcca atccccggtc ggcaaaggcg cgcaccttgg
     1741 aaaccgcaac catcgcctgc gctgaagcgg gcgtgatgta aatttccttg acgttcccct
     1801 gccaagccgg actggccggc tgatagccgc gccagagctc gcgcggcagt tcttcgggca
     1861 tcggtcgaat cgcctgggcc atggatcctc ccctaaacga caccggccac tgatttctca
     1921 gtggccggcg gttcccgaca tagattatac ggatcggaac tttatcccgc cgcgtccgcg
     1981 tagaaacgcg tgtcttcgat cggcggcacg gtcttgttct cttcggagga agcgattaac
     2041 ggtttgccgt ccggacgcac gcgcatctct tcgccgccct gggacttgcc cttgatgcct
     2101 gcgccgatgc gcagtacgac caggttttcg ctgccggtgt tgagatagcg gtagtacgcg
     2161 cccttgggga tcatgacgcc ctggtgcttt tcgactttga tctcgccgcc tttttcatcg
     2221 aacacgctca tctggccttc aagcacgatg aatgagtgat cttcgtcgag atgacagtga
     2281 atctcgttct cgccgccctc gtagttgatc ttggtgtgga tccacatgtt ctcggtttcc
     2341 gccaccaatt gggtgatgcg gccctgcttc atgtagggag tgcgcagaga aaacttctgg
     2401 gtgtggggtt tctccgtcgg tgctaatttc ttcgctggtt gcatgattgt tcctttcctc
     2461 ggtttggttt tagttgttaa tgccgtcgac tttgatgtga tacaaatcca ggacgttttg
     2521 ccgcaccact ttgtcgatga cgttcttggg cagatggccc aactcgtcgg tgatcacttg
     2581 gcgcgagtgc ggccaggtcg aggcggcgtg gggataatcg ctcgaccaca taaaattgtc
     2641 ttcgccgaac caatccatca tgcggccacc ggccgggtcg ctgaagaagg tcgcgaatac
     2701 ttggtctttg acatattcgc cggggagctt tttcatatac gccagcggcg ctttcgggct
     2761 gtggcggacg tagtacttat cccattcatg acaatagaat ggtatccagc ccacttcgtt
     2821 ttcgacgtag accaacttga gtttgggaaa cttcgccaag ggaccgccaa agacgaagtc
     2881 gaacaaggtg ttcgccgcat cggtgagctt ctgattgacc gtcatgcggt aagtatccaa
     2941 gcctttacgg tcgaaacgac tgtagttgaa gccggtcaag atatgcacgt tcaccggcat
     3001 atcgagttct cccgccgctt cccagaatgg attgtaataa tcgctggtga atggcagatg
     3061 gtccggcggc acctgccaga tcatgcagcc gatcatgccg ttcttcttgc agcgttcgag
     3121 ttccttgatc gcgttgggga tgttgtacat cgagatcatt gggatgccga acagtcggcc
     3181 gggggcggct ttgcagtatt ccatcatcca atcgttggcg atttcgaagc acgcctcttg
     3241 gagcggcgcg tcttccaggg cgtaaaggcg caagccgtgg gtggcgtaga gcacctcggc
     3301 gctgacgccg tcgacagcca tctcatccag tcgcgctttg ggatcccagc cgccgggctt
     3361 ctcgccggcg gtattgcgcg gcggaaattt cgggaatttg gccgccaagc tcggcgtaag
     3421 attcttcagc cagagatcct cgggctccat gatatgcgag tccgacgaca cgatcttgtc
     3481 ctggccaaga acctgctccg cggtgcgctt gactcctgta gcagtagtca tgagtagtcc
     3541 tcctaatagt taacggttat ttccttttga tctattcctt ttttcgccgt ttgtccaggc
     3601 caaatcggct gtccgcagat tggactttgg ccaaaccatg gcgtagaaca tcttgaccat
     3661 catgagatcc ctcaagggtt tattttcgca ctgtttagcc gtcctgttga tccccgccgc
     3721 gctgttcggc gccgaggcgc aaaacgcctt caacgaaggc gtgaagcatt acaaagcggc
     3781 caagttcaaa gaagctgtgg cggcgtttaa ccgcgccatc aaagcggcgc cgaaatccgc
     3841 cgaagcctac aacaatcgcg gtctggccta tgccaagttg ggacagccgg aaaatgcgct
     3901 aaaagactac gacgaagcac tcaagttaaa ccccgagctt accgacgccc agttcaaccg
     3961 cggcaatgta ctgttcaagc agaaacaata cgaacgggca gtcaaagact ttgacgaagt
     4021 gatcaagcag gacgccaaag cgccgcgcgt cttcgtccag cgcggactgg cgtttttgca
     4081 actcaaacaa ttcgacaaag cggtgcagga cttcaaccag gcgcagaagc tcgacccgaa
     4141 ggacgccgag atctattaca accgtggcct ggcccatcgg cgcctgggca agaacgaatt
     4201 ggcggtgcag gactatagtg aggcgatcaa gctcgatccg aaaaacggcg agaactacaa
     4261 caaccgggcc aacgcctatt cgttgctggg aaaatatgac gaagcggtga aagactatga
     4321 cgaggcgatc aagctcaacg acaaggatgc cagaatctac gccaaccgcg gcgtcatcaa
     4381 acagcggctg aagaaggatg acgaagctca gaaggatttt caaagggcag tggaattgga
     4441 tcccggtctg aaagataaac tcgcctcgtt tatcgctccg gcaaaaggca aataaatcat
     4501 ggcgcgcgcg ctggtgataa tcggctactt actcgggctg accgccggcg cggttccggc
     4561 ctgggccggc ctggcggacg atccgctgaa aaccgctctc gagctgcacg aaagcggcaa
     4621 ggtcaaggag gcggtcaccg aatacgacaa agtcatcaag acgaatccca aactggccga
     4681 agcctatttc aatcgcggca acgcccattt cgacttggga cagaaccaac aggcggctaa
     4741 agactacagt gaggcaatcc ggctcaatcc aaaggacgcc gaggcctatt tcaatcgcgg
     4801 caatacctat cggcgcctga aacaggacgc cgctgctctc aacgattaca gcagcgccat
     4861 caagctcaat cccgacgacg ccaagagtta taccaatcgc gccactattc acttcgctgc
     4921 gcgccgttac gaagccgcct tggcggatca aaaccaggcg ctgcgtatcg acgccaacga
     4981 cgcccagtat tatttcaacc gcggcaacag ctatctggcc ctcggcaaaa aagaagaagc
     5041 gatcgcagac tacaacgccg cgctcagccg cgaccccaag ctcgccgatg cctattacaa
     5101 ccgcggcatc gcccacgccg actcgaacaa acaggaagag gcggtgggcg acttcgacct
     5161 cgcgatcaag ctcaatccga aaaaccccga ggccttctac aatcgcgggc tggccaacgg
     5221 caaactaggc aagcatgatg cggccatcga cgactacggc gaagcgatcc ggttgaaccc
     5281 caaggatgcc gaagcgccgt tcaatcgcgg cctcgcctat atgcgcgtcg gtaagcacga
     5341 ttttgcggcc cgcgacttca ccgaagttat cggtctggaa ccgaaacatg ccgacgctta
     5401 cgtctatcgc ggtctcatcc gggtttatca agggcgcgag aacgacgctg acgccgattt
     5461 tcaacgagcc tatcggttga atccggcgct caagaaaaaa ttggaaccgg tgatcaccga
     5521 ggccaaagcc aaggcgaaga atatcgccaa gtaaaccgag caaactctca tggcggaaag
     5581 caatttcttc ggcggtttga ccggcggcct gctgatcgga ctcgccgctg cactgctgtt
     5641 gctctttaat ggccgcatcg ccggcatctc gggcatcctc ggcggcctca tcgatgctaa
     5701 cccgatcagc gaacggctct ggcgcggcgc ctttatcatc ggcctggtgt gcggtgcggc
     5761 agtctactcc tggattcatg gcggtctgcc gttgcaactt caagtcagaa gttccggttt
     5821 actgctggcc ggtttgctgg tcggcttcgg caccagacta ggctccggct gcacctccgg
     5881 ccatggcgtg tgcggcttag cgcgctcctc gccgcgctcg ctggtcgcca ccgcgacctt
     5941 catcgcttgc ggcatgttga cggtgtattt gaccaggcat gtattctaat gctgccgctg
     6001 atcagcagct tcgccctggg cgtcctgttc gccttcggac tggcgatttc agaaatgatc
     6061 gacccggcgc gggtgatcgg cttcctcgat gtcgccggcc gctgggatcc gacgctccta
     6121 ggcgtcatgg gcggcgcgct ggcggtgacc ctgccgctct ttccgtggat tcagcaacgc
     6181 cggaaatcgc tcctcggtga accgttccac ttgccgagcc aggcgcgcat cgatgcgcgc
     6241 ctgatcattg gcgccgcgct cttcggtatt ggctggggtc tcgccggtct ttgccccgga
     6301 ccggcaatag ctggcctggc cagccgctcc ggcggagtgt ttctgtttgt cctggcgatg
     6361 atcgccggtc aatggctggc ggcgcgctcg actcgcgcta actgagggcg atgaggggaa
     6421 tcgttcttga ctccggcccg ccgagagcgc tagctggggc ttgcagcatc gctttggcaa
     6481 ttgatctcca agccgctgaa taactttcgc ttgtaggact ttttcaatgg gtgagacgcg
     6541 cgtcgatctg caacatttgc tcgaagactt gcgggacgcc taccctggct ccatcgagga
     6601 aaccattctc accgagatca tggccaactc gctcgactgc ggcgcgacca cgatccaact
     6661 aatcaccgat cccggcgcac gcacattgac cgtcaccgac aacggtagcg gcatgcagcg
     6721 ccgcgatttg gcgcgttttc acaacatcgc gtcgagcacc aagagccgcg gcgaaggcat
     6781 cggatttgcc ggcgtcggca tcaagttggg gcttttggtc tgtgacgaag tgttcaccga
     6841 aacccgccgg ggcaaaactc atattgccag ctcatggcat ctggcgacgc gcaatcgcgc
     6901 gccgtggaaa tgggtggcgc cacttaagct gatcgccgaa cgcggcacgg cggtgcgctt
     6961 gcaagtcgag aatccattat cgccgctgct cgatccgggt tttttagcgg tgaccctgcg
     7021 gcgacatttt cagcccctgc tggaccccag cttcgacgag tttttgcgcg gccattatcc
     7081 cagtggcgtc gccatcgagg tcgacggccg ccggctagac aaacagcgct ggagcgcgcc
     7141 gcgcgcggcg ccgttggaaa ttcgcctcgc gcgcaaacgc aaagccgccg cttcgggcta
     7201 cctgattcgc gccgatcatc ctttacccga ggagcgccag ggtttggcga tcagcaccta
     7261 tgggaaagtc atccggcgcg gctgggactg gctgggtgtg acgccagcag cggccgattg
     7321 cgtgggcggc atcatcgaag tgccggagct cgccgcctgc ctgactctca acaagggcga
     7381 cttcatccgc tccggcgccc gcggcgcgac ctatttagga tttcgtaaag cgatccaaga
     7441 agcggtcggc aggcaactcg ccctctgggg cgatggccgg agcggcgccg aacaagcgcc
     7501 accacgggaa gtgcggccgc tccagcgcga cctcgtgcaa gtattggagg atcttgcgct
     7561 ggacttcccc ctgctcggct ccctggtcga gcacagagcc ggcggacaaa aacgattgcc
     7621 gctgggcata cgcggcgccg gcagcgatgg ccatgggttc gtcgcggcat cactcaccgc
     7681 ggcggcggag agcgaaccga ccgagaaccc gcgagcgccc gcggcagctt caacggattc
     7741 aagcccggcc actgcgtcag cagtggtgca tgaagctaac gatggctacg gcaccgacgg
     7801 cagctcaatt caattgccga gtaagtccat cgtccgccgt cccgcgcatt acggcttgga
     7861 cattcaattc gcagacaggc ccgacgacat cgaactgggc cgcttgaccg aatcgactgt
     7921 ctgggtcaac cgcgcccacc cggcttaccg gcgcgcccaa gcctcgcatt cgttcggtta
     7981 ccacatcgcc ttagcggtgg cgatggcgct ggcgccgctt gccgtcgagg cgccgcacca
     8041 acatggattt gtctccgcgt ttctttcacg ttggggtgag gccatcgata aacccaaagc
     8101 gcgcagccgg cactgaggga tttgcatacc tctttcggcc ctgcttgacg gccttccgtc
     8161 atgcccgccc atcgacgcgg ctcaggataa attccgatcg gcatccatct gcgtttctga
     8221 cacacccaaa cacgcctgga tgccgttgcc ggatcaagtc ctggactctg ccccggaacg
     8281 acgggaaaag ccgtcgacct ccatcagggc ttcaagccca gcagcgagag aaattcctga
     8341 ttgtactcgg cgtaacggtc ggcgatcgac atgtccaccg gcgcgagttt cgattgcatc
     8401 atgcgttcga gccgcttgtt tttgatcggc acatccttgc gcgccggcac ccggtcggag
     8461 atttcattca tcgctttgag cccctcgacg gatagtcccc aatagagaaa caatcgtgcc
     8521 gcgttgggat gaggcggctt ggcgccgatg ccccacgcca ggacgttcac cggcaccggc
     8581 tcgaaggcga cccaatcgat cggcgctccc ttggcgcgaa tgttttcgat caccgacgca
     8641 taggcgctga tgccgagggc aaactcgccg gcgctgacca gctgcgcttc catggtatga
     8701 ccgcgccgcg gccgcagacc gttggcgacc aattttttaa accattcgcg ccccttgtca
     8761 cggcccatga gtttgagcca agtggcgtag agatcgacat cctgctcgtc catggtcaat
     8821 ttgtctttcc acttgggatg ggccaggtcc tcataactct tgggcaaatc gttgggcttc
     8881 agcatgtttg tgttataggc caccgaccag aattgaatgt aatacgccgc ccaatagtcg
     8941 tccggatcca tgaagccgtc gagaaagcga ttgcgctcgg gatagttcat cttctgcagc
     9001 cagtttttgc gccggtacag ttcgagattg ctggcgcgat tcataaccac atcgaagccc
     9061 cagcggccgg cgggaatttc agtttgcagc ttctgggtca tcgccgtggc gccggcgcga
     9121 aacaggctga ccttgatgcc gtattttttt gtaaagagcg ccgcccaggc ctgggtttcc
     9181 ggcagcggca aggtcgaata ccaaaccact tcgccttcgg ctttggccct atcgactaaa
     9241 tcgctggccg agatctcggc ggcatgggca ccggcgccgt gaacccagcc gaatatcagt
     9301 agaacaatcg ctcgagcatg gttgcaaaac atggctttcc tttcattctt caatcccgca
     9361 ccacgctgcc ggcgctggtg ccaacccggc gagcgctcag cgcatcgcgc aggatgcctt
     9421 tgggatctcg tcctgcgcgc acttcaccaa tcgccgcgcg caacagtttt ctaaacagca
     9481 tgactccctt gtcggagctg accagatgct ccaggccatg cagggcgatc ggcccctgcc
     9541 cttcgacggc ttccttgtcg tcgggatgac gctgggtgta ctcgaaactg ctgtccttgc
     9601 gcccacccgg cgccaactgt tcgcggccgg acaacgattc ggtttcattg tcggcgagcc
     9661 agcgcacgct ggcgcccaat tggtgggtgt cgtcgaccgg cacacagtag gtgaccgcgc
     9721 gcacgcgctc ggtccgcggc gcatcggtat aaaccaaatg gacattgagc ggcatgagca
     9781 tttcccagat gaggtcccac catttgccgt ttgccaattt gcgcgtgagg ataaaacgca
     9841 tgcccatagc cgtctcttcg tatttcaaat catcgggagt cgcgccatag gcttgggagg
     9901 gaaactgcgg cccgctgtgc agcgtgtgca accaaaaggt gtgcagcgcg tccatcaaat
     9961 tctcttcggc ttggagccaa ttgcaattga ccgggccgcc aatccgcggc cccatgcgcg
    10021 ctttgagtgt cccgccggca gtttgccaga tatcgtaaag gggaaactcc ggctgccgct
    10081 ccaacgggcc catgtaggcg aacagcaggc cgccaaattc acgcaccggg taagccggct
    10141 gttggatgtg cggcaaaaat gggttgggcg ccggctcgag gggcatctcg acgacggtgc
    10201 cttcgacgtc atagagccac ccgtggtaac agcagcgcag gccacgcggc tcgacccggc
    10261 cgtattccag cgacgcgccg cgatggctgc aacgaaaaaa caatagcccg gcgcgcccct
    10321 ggccgtcgcg gaaggcgatt aactcttcac cgagaatctt gaccggcttg ggcaaatcct
    10381 tgagatcggc ggagagacag accggttgcc aatagcggcg catgagttcg ccgcatggcg
    10441 tgcccggtcc gacacgagtc aactcgccat cgggttcggg aattttgaga ccgtaagcgc
    10501 tcttcatgtc tgccgccggg accgaacaat accgcggcga tcatatgccg cagttatccg
    10561 gagtcaagat ataacctgcc gcttgcttga cagcg
//
LOCUS       DASQKS010000302         6061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2690779, whole genome shotgun sequence.
ACCESSION   DASQKS010000302 DASQKS010000000
VERSION     DASQKS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690779"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1230)
                     /locus_tag="VE170_07850"
     CDS             complement(235..1230)
                     /locus_tag="VE170_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ15386.1"
                     /translation="MDQKGILYLSNNDVKKVLDLGRAIEITEQALRDHSEGRVTWSTP
                     EDFAIKPEQGWQSWVTGCALQTSPVAGFRIRSIKAAGGSRDVSRPPRGPRRVLILSDL
                     DGGEILSFMDEDWCHAVRTAAAATVAMRVLARKDSTVMAMLGAGDTARAAVPVMAQAF
                     NLKEIRVTSRTPESRQSFAREISKEYGLNVVPVESTEAALDGADVVISATTTPTPFVK
                     DSWLGAGITVYSIGKNQEVESEVYKNADKFIVDSWLHCKNKSDMQRMLKENYLSKNDL
                     YAELPELLSSKKPGRQSDQERIFIRAIGLVNQDIAMADHIYRSAREQGIGTRLPY"
     gene            complement(1401..2537)
                     /locus_tag="VE170_07855"
     CDS             complement(1401..2537)
                     /locus_tag="VE170_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ15387.1"
                     /translation="MATESPFVDQEYFDRQGRAQALMERDGLDALIVSEKNNYWYFSG
                     LISYQLDHIQRPQICILPKSGKPLLLVYGNDKAKAKALPWVGEVRSYTDVPFPREMIA
                     ASIKEMGLGESKLGFELGDDQRLGIPVNYLSGLTEALPKAQIKDGSAALNEMRIIKSP
                     LEIGFLRKACEISVKAYDRCLPQLKPGMTRREVADRLYISMIQEGAHPRHPGFLMLNS
                     STRYDDRRYDKGDRMIADFGACYEGYYGDITRMAIFGEPSAEHKKDHETACDVIQLCF
                     ESMKVGTPLAEVSRVANRELIRRGYQAVDSPKRIGHGIGMSRAEPPSLNEVETETYRP
                     GMVLALEPKVRSATSAVHLEEDVLITEKGQEFLTSGCRRLDVIN"
     gene            complement(2599..3306)
                     /locus_tag="VE170_07860"
     CDS             complement(2599..3306)
                     /locus_tag="VE170_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HYJ15388.1"
                     /translation="MREWETFVPEEERKIYEKAGYKGKEKFGANPALIIIDVITGFTG
                     TKPMPVMEAIDEFPTSCGQVAWDALPRIKELLHACRDAEIPVIYSTSDPDFKAAFGNA
                     TKRGVDKTDFEKLAVEFPEMIKPRDNEFIVRKARASAFFGTHLITYLVRKNIDSLLIA
                     GTSTCGCVRGTVLDGYSYGYPVFPVEECIFDRSRTSHLVNLFEMNAKYASVIQLSEAL
                     DYVGKIKRMEGRKAIAS"
     gene            complement(3324..4322)
                     /locus_tag="VE170_07865"
     CDS             complement(3324..4322)
                     /locus_tag="VE170_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15389.1"
                     /translation="MTHLVGLFLWLITLVPAAAIGADASLIEAAKREGSVVFYTTMDI
                     QNSSALVDAFSKKYPFIKGDLVRLGGTAMVSRIMTEAQAGANRFDVAVGISPSFTPMR
                     ERNLIAPYLSPEFTNLYDDLYDPKGYWATVYLNTLVLGYNSKAIPRGELPKTYDDLLK
                     PQYKQKFIIDIENHDVFVALSQEWGQDKAVNYFKALAKQIPIFLRGNTNRANFVSVGE
                     RAMTFVYAQVIERMKQSGAPVDWIPLEPVAVELNIAMLSAKAAHPNAARLFLDYMISR
                     DGQEFLKTFRRIGPRKDVKPDPPKLFEGFQRRVVSPDAYKNLRELTKLHNDALGVR"
     gene            complement(4389..5456)
                     /locus_tag="VE170_07870"
     CDS             complement(4389..5456)
                     /locus_tag="VE170_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ15390.1"
                     /translation="MVLVLKNHQMENLLPMAEEIDAIELAFRELGQGKAMLAPRARLR
                     TPWKEEGGQYYFNNIMGLVPGMKSMALRIDSSFSKEIEVGGSKRRVYPGDFIGLVMLF
                     DMDTCNILAIMDDHYISTMRVGATSAVASNYLARKDAGIMALLGSGEQAKTQVTAHAC
                     VRKLTKVKVYSPTKANREKFAKEVSAETGIEVIPMDSAEAAVRGSDIVTAATNTVDPV
                     ILGKWIEPGMHLNSIVGGDGFLPRKELDDEAVLKAHLIVVGYKAQIFLDKQAEFHDRL
                     ERGLVKAEDLHELGELVNGDCRGRQSEQEITFFKNNTGMGIQFAVTARKMYDKAKEKG
                     IGTELPLELFMTQRGDKLFSP"
     gene            complement(5605..5877)
                     /locus_tag="VE170_07875"
     CDS             complement(5605..5877)
                     /locus_tag="VE170_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HYJ15391.1"
                     /translation="MTYIIAEPCIGTKDTACVDVCPVDCIHPTKNETEEFEKETKLYI
                     DPEECIDCGACEPVCPVEAIFEEAAVPDKWKQFIQIDADWYKAKKG"
BASE COUNT         1196 a         1637 c         1752 g         1476 t
ORIGIN      
        1 aaccttcacc ggctgggcat cgaccattgc cggcgcactc aaggcgaatg tgtaaagcag
       61 agcgacgagg ccaagccatt tgggcttgcc gaaagaatgg tttaaccatt gtgtctgggg
      121 tttttcgttc atgctcttcg ccctttaaga atggtgattg cgaatgcctt ggaatagcca
      181 attggctgat gacgccttgg tagggtagtg ggcgcccaaa tccacgcagc aacctcaata
      241 cggcagccgc gtgccgatgc cctgttctct ggcgctgcga taaatgtgat cggccatggc
      301 gatgtcttgg ttgaccaggc cgatggcgcg gatgaagatt cgttcctgat ccgattgacg
      361 cccgggcttt ttgctggaga gtaactcggg cagttcggcg tagaggtcat ttttgctcaa
      421 gtaattttct ttgagcatgc gctgcatgtc ggatttgttc ttgcagtgga gccagctgtc
      481 gacgatgaac ttgtcggcgt ttttgtaaac ttcactttcc acctcctggt tcttaccgat
      541 ggagtaaacg gtgatgcctg cgccgagcca gctgtctttt acgaatggcg tcggtgtggt
      601 ggttgccgag atcaccacat cggcgccgtc aagcgctgct tccgtggatt caactgggac
      661 cacgtttagt ccgtattcct tgctgatttc cctagcgaag ctttgacgcg actcgggcgt
      721 gcgcgaagtg acgcggattt ctttcaaatt aaacgcttga gccattaccg gcactgcggc
      781 gcgggcggta tcgccggcgc cgagcatcgc catcacagtc gaatctttgc gcgcgagcac
      841 acgcatggcg acggtagccg cggcggcggt gcgcacggcg tggcaccagt cttcatccat
      901 gaaggacagg atctcgccgc cgtccaagtc actaagtatc agcacgcgtc tcggtccccg
      961 cggcgggcgc gaaacgtcgc ggcttccgcc ggcggctttg atggagcgaa tgcgaaagcc
     1021 ggctaccggc gatgtttgca aggcacaacc ggtaacccag gactgccagc cttgctctgg
     1081 tttgatcgcg aaatcttccg gcgtcgacca ggtgacccgc ccctcgctgt ggtcgcgcag
     1141 cgcctgctcg gtgatctcga tggcgcggcc gaggtcgaga acttttttaa cgtcgttatt
     1201 tgacagatag aggatgcctt tttgatccat ggatggcccc gtatgatgat cgttgttcgc
     1261 ggtcggcaaa gattttaccc gcgccgttag taatataggg ttacggtgag gttcgagttc
     1321 gctgagtcag agtcctcgct ctgcctcttc cagtgggaat gactaagcat tggaggcgga
     1381 cagtttccgc caccggtaag ttaatttatc acatccaatc ttcggcaacc gctggtcaaa
     1441 aactcctgtc ccttttcggt gatcaaaaca tcttcctcca aatggactgc gctcgtcgcc
     1501 gagcgcactt tcggttccag tgcgaggacc atgccgggac ggtaggtctc ggtctccact
     1561 tcattcaatg acggcggttc ggctctcgac atgccgatgc cgtggccaat gcgcttgggg
     1621 ctgtcgacgg cttggtagcc gcgcctgatc aattcgcggt tggcgacgcg ggagacttcg
     1681 gctagcggtg tgccgacttt catggactcg aagcagagct gaatgacatc gcaggcggtt
     1741 tcatgatctt tcttatgttc ggcgctgggc tcgccgaaga tcgccatacg ggtaatgtcg
     1801 ccgtagtagc cttcgtaaca ggcgccgaag tcggcgatca tgcgatcgcc tttgtcatag
     1861 cgccggtcgt cgtagcgggt tgacgagttc aacatgagaa atcccggatg gcgcggatgg
     1921 gcaccttcct ggatcatcga aatatagaga cggtcggcga cttcgcggcg ggtcatgccg
     1981 ggctttagtt gcggcaggca gcggtcgtag gctttgacgg aaatctcgca agctttgcgc
     2041 aggaagccaa tttccagcgg gcttttgata atgcgcatct cattcaatgc ggcgctgccg
     2101 tctttgattt gtgctttggg caacgcttcg gtgagtccgg acaagtaatt caccggtatg
     2161 cccaggcgct gatcgtcgcc tagctcgaag ccgagtttgc tttcgcccag gcccatctcc
     2221 ttgatgctgg cggcgatcat ttcccggggg aaaggcacat cggtgtaaga cctcacttcg
     2281 cccacccagg gcagcgcttt ggccttggct ttgtcattgc catagaccag caggagcggt
     2341 ttaccgctct tgggcaggat gcagatctgt ggccgctgga tgtggtcgag ctggtagctg
     2401 atcaggccgc tgaagtacca gtagttattc ttctcggaaa cgattagggc gtcgagacca
     2461 tcgcgctcca tgagcgcttg ggcgcgcccc tggcggtcga aatattcttg atccacgaac
     2521 ggggattcag tggccataag ctgcgctcct attctatgcg tttgagtttc gaagcgggtt
     2581 cgcgcgggat cttcgacctt aactggcaat cgcttttcgg ccttccatgc gcttgatctt
     2641 gccgacgtag tccagggctt cggacaactg gatgaccgac gcgtacttgg cgttcatctc
     2701 aaatagattg accaggtgtg aagtgcgcga gcgatcgaag atgcattctt cgaccgggaa
     2761 caccgggtaa ccgtaggaat agccgtcgag gacggtgccg cgcacgcagc cgcaggtgct
     2821 ggtgccggca atcaacaggc tgtcgatgtt tttgcgcacc aagtaagtga tcaaatgcgt
     2881 gccgaagaag gcgctggcgc gggccttgcg cacgatgaat tcgttgtcgc gcggtttgat
     2941 catttcggga aactccacgg cgagcttctc gaaatcggtt ttgtcgacgc cgcgtttggt
     3001 ggcgttgccg aaggcggctt tgaaatcggg atcgctggtg gaatagataa ccgggatttc
     3061 ggcgtcgcgg caggcgtgga gcagctcttt aattcgcggc agcgcgtccc aggccacctg
     3121 gccgcagctg gtgggaaact cgtcgatcgc ctccatcacc ggcatgggtt tggtgccggt
     3181 aaagccggta atgacgtcga tgataatcaa cgccggatta gcgccgaact tttctttgcc
     3241 tttgtagccg gctttttcgt aaatctttcg ttcctcttcc ggaacgaatg tttcccattc
     3301 cctcatggtg catctcctct gggttaacgc acgccaagcg cgtcattgtg tagtttggtc
     3361 aattcgcgca agtttttata agcgtcgggc gacaccacac ggcgctgaaa accctcaaac
     3421 agtttcggcg gatcgggttt gacatctttg cgcggcccaa tgcggcggaa ggttttgagg
     3481 aattcttggc cgtctctgga aatcatataa tcgaggaaca agcgcgcggc attgggatgg
     3541 gccgctttag ccgaaagcat ggcgatgttc aattcgacgg cgaccggctc taagggaatc
     3601 cagtccaccg gcgcgccgct ttgcttcatg cgctcgatca cttgagcata aacaaaggtc
     3661 atcgcgcgct cgccgacgct gacaaaatta gcgcggttgg tgttgccgcg gagaaaaatc
     3721 ggaatctgct tggcgagcgc tttgaaatag ttgacagctt tgtcctggcc ccattcctgg
     3781 gacagcgcga cgaacacatc gtgattctca atatcgatga tgaatttttg tttgtattgc
     3841 ggcttgagca aatcgtcgta ggttttcggt agttcgccgc gcgggatcgc tttgctgttg
     3901 tagcccaata ccaaggtgtt caagtaaacc gtcgcccaat atcctttggg atcataaaga
     3961 tcgtcgtaga gattggtgaa ttccggcgaa aggtagggcg cgatcaaatt tcgctcgcgc
     4021 atgggtgtaa acgatgggga aattccgacg gcaacatcga agcggttggc gccggcctgg
     4081 gcttcggtca tgattcggct caccatggca gtgccgccaa ggcggaccaa atcacctttg
     4141 ataaacggat atttcttgct gaacgcatcc accaaggcgc tgctgttctg aatgtccata
     4201 gtggtgtaaa acaccacgct accctcgcgc ttcgcagctt cgatcaacga agcatcggcg
     4261 ccgatcgccg ccgctggcac gagcgttatc agccacaaaa acagaccgac taaatgcgtc
     4321 ataaatcctt caacccatcg ggttcattaa ttaattgatc atcggccgcc tcgccgtaga
     4381 tagccagcct atggggaaaa cagtttgtcg ccgcgctggg tcatgaacag ttcgagcggc
     4441 aattcggtgc cgatgccttt ttctttggct ttgtcgtaca tctttctggc ggtgacggcg
     4501 aactgaatac ccatgccggt gttgttcttg aaaaaggtga tttcctgctc gctctggcgg
     4561 ccgcggcaat cgccgttgac cagctcgcct aactcgtgca ggtcttcggc tttgaccagg
     4621 ccgcgttcca ggcggtcgtg aaactcggct tgcttgtcga gaaaaatctg cgctttgtag
     4681 ccgacgacga tgagatgggc ttttaacacc gcctcgtcgt cgagctcttt tctcggcaag
     4741 aagccgtcgc cgccgacgat actgttgaga tgcataccgg gctcgatcca tttgcccagg
     4801 atcaccggat cgacggtgtt ggtcgccgcc gtaacgatgt cgctgccgcg gaccgctgct
     4861 tcggcggaat ccatgggtat gacttcgatg ccggtttctg cgctcacttc cttggcgaat
     4921 ttttcccggt tcgccttggt cgggctgtag actttgacct tggtaagttt gcgcacgcag
     4981 gcgtgggcgg tgacctgggt tttggcctgt tcgccggaac cgagcagagc catgatgccg
     5041 gcgtctttgc gcgccaggta attgcttgcc accgcgctgg tggcgccgac gcgcatggtc
     5101 gagatgtagt gatcgtccat gatcgccaag atattgcagg tgtccatgtc gaagagcatg
     5161 accaggccga tgaaatcgcc cggatagacg cgccgcttgc tgccgccgac ctcgatttcc
     5221 ttggagaagc tcgagtcgat gcgcagcgcc atagacttca tgccgggcac cagccccatg
     5281 atgttgttaa aataatactg gccgccttcc tctttccacg gagtgcgcag gcgggcgcgc
     5341 ggcgcgagca tcgccttgcc ctggcccaac tcgcgaaacg ccagctcgat ggcgtcgatt
     5401 tcctcggcca tcggcagcaa attttccatc tgatgatttt tcaagacgag gaccatgcag
     5461 atctccttag atgctctgcc tacaacagag ctcgaagtgg agtcaaggct gcagcgaatg
     5521 taaacacagg agtagaaaaa ccaagggctg agaaaatatt ctcagccctt ggtaaaaccg
     5581 tgtgaaatag atggtgagag gcggctagcc tttctttgct ttgtaccagt cggcgtcgat
     5641 ttgaataaac tgtttccact tgtctggaac cgccgcttcc tcaaagatcg cctctacggg
     5701 acagaccggt tcgcaagcgc cgcaatcaat gcactcctcg ggatcgatat agagcttagt
     5761 ttctttctcg aattcttcgg tctcgttctt ggtcgggtgg atacaatcaa ccgggcagac
     5821 atcgacgcag gcagtgtctt tggtcccgat gcagggttca gcaatgatat aagtcatcta
     5881 ttcctcctag attcagactg caacctagtt agcaaatcga ctctcgcatg tcaatttatc
     5941 ggcacttccc gcggcgttga aatcgcccgc caaccgcccg ccttgatcct ttgaaccggc
     6001 tgatgttaga taaacctact cgagatcggc ggaaccatca tgctgcagct ggttttttgt
     6061 t
//
LOCUS       DASQKS010000303         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4779123, whole genome shotgun sequence.
ACCESSION   DASQKS010000303 DASQKS010000000
VERSION     DASQKS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4779123"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VE170_07880"
     CDS             complement(<1..214)
                     /locus_tag="VE170_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HYJ15392.1"
                     /translation="MTKAEIITRIYEKVGFSKKDATDVVEATFDIIKGSLEKGDKVKI
                     SGFGNFVVNTKRPRKGRNPQTGDEIII"
     gene            complement(252..2330)
                     /gene="pheT"
                     /locus_tag="VE170_07885"
     CDS             complement(252..2330)
                     /gene="pheT"
                     /locus_tag="VE170_07885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYJ15393.1"
                     /translation="MKFTLNWLKEFVEFGGTAGELSRLLTSAGLEVESLTPLEEPEGH
                     RQDWLFEIAVTPNRGDCLSLLGIAQEVCALTGTRVKFPPVPASKRDPRMHEHVALNIE
                     DVSQCARYSARIVDDVKIVRSTPLLRFRLESCGIRAINNVVDATNYVMIETGQPLHAF
                     DLAQLPAKRIVVRSAREHRKFTTLDGVERELAHDDLLICDGDTPVALAGVMGGMNSEV
                     SAATTSLLLESANFAPATIRRTAKRLALHSEASYRFERGVDPEGTLRALDRAVQLIGE
                     NGGGRPLPGAADCYPEKVDPVTVCLREQRISRILGVPMELRQAEKLLRSIGLKTTRQA
                     QQLTVVIPTRRPDLTREIDLIEELARLQGYDNIPITLPSLRPTGGAPDFRLASERKLR
                     NFFAAEGFVEAVTLPFTSEAINGLFPGLWLGSGEAVPLVNPLAKENSALRRSLLPGLI
                     DTLRVNLAQKAASLHVYHLGKVFRCNPNGGYEERLCVGGLMHGARAVHGLRRKDEPKL
                     DFFHCKGAIEGLLELFGVRDGLAWNALASDLLHPGRAAQWTYKSCPMGLLGELHPDVC
                     ERLEVPQVYVFELDFEKLLEYAPRHMGVRPLAKFPAIERDFAIVVDRDFAAYRIIDWL
                     DHLGEPLIEHVQVFDQYLGAPIPDGKKSLAYNISYRAEDRTLTDSEVNPLHQSVVDRI
                     GKEFGAERRS"
     gene            complement(2421..3461)
                     /gene="pheS"
                     /locus_tag="VE170_07890"
     CDS             complement(2421..3461)
                     /gene="pheS"
                     /locus_tag="VE170_07890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYJ15394.1"
                     /translation="MTDSLERLRGEVLERIQQAESIKELEQLRIEALGRSGSITLLLR
                     GLKELPAEERPRMGERLNQLRRALEQELDARLAVVNRQARAKAFAEEKIDISLPGTRL
                     ERGHSHPLTLVIDEIVEIFSAMGFEIARGPDIEDDYHNFEALNIPKDHPARDMQDTFF
                     VDGGGLLRTHTSPVQIRTMESRKPPLQIIVPGAVYRHDDDATHSPMFHQVEGFMVDSH
                     IAFADLKGVLTHVLRQIFNRDAGVRFRPSFFPFTEPSAEIDIQCVICGGGGTLRNGQS
                     CRVCKASGWLEILGAGMIDPAVFKFVGYDPEEFTGFAFGMGVERIAMLKYGIDDIRLF
                     FQNDLRFLRQFS"
     gene            complement(3483..3842)
                     /gene="rplT"
                     /locus_tag="VE170_07895"
     CDS             complement(3483..3842)
                     /gene="rplT"
                     /locus_tag="VE170_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HYJ15395.1"
                     /translation="MPRAKGGTKTRQRHKKIMKLAKGYVGGRGRLYRSAVETVERGLV
                     FAYRDRRVKKRTFRGLWIIRIGAAAKLNGMSYNQVINGLKKANVEIDRKILAEIAVAD
                     MPGFGRLAETAKAHLSQ"
     gene            complement(3946..4143)
                     /gene="rpmI"
                     /locus_tag="VE170_07900"
     CDS             complement(3946..4143)
                     /gene="rpmI"
                     /locus_tag="VE170_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HYJ15396.1"
                     /translation="MPKIKTNRGAAKRFRVTGSGKVKRNKAYKRHILSSKGKKRKRNL
                     RKGTMVAAVEAKNIRQLIPYK"
     gene            complement(4177..4677)
                     /gene="infC"
                     /locus_tag="VE170_07905"
     CDS             complement(4177..4677)
                     /gene="infC"
                     /locus_tag="VE170_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYJ15397.1"
                     /translation="MAREARINHQIRAREVRIIGSKGEQLGVMPLHEALKQVEALDLD
                     LVEVAPDAQPPVCRMMDYGKFRYEQKKKQQGPKKTVVEIKEVKMGSRTDAHDIEHKVK
                     NIRKFIDEGQRVKVSVFFRGREITHPELGRHLLTGVVELVQDVAKVDIQPRLEGRNMA
                     MLLTPK"
     gene            complement(4721..>6505)
                     /gene="thrS"
                     /locus_tag="VE170_07910"
     CDS             complement(4721..>6505)
                     /gene="thrS"
                     /locus_tag="VE170_07910"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYJ15398.1"
                     /translation="LVDLNRIIDKDCTVEWISPESAAGLDVLRHSSAHLMAQAVQSLF
                     PGTQVTIGPTIDDGFYYDFKRDQPFMPDDLERIEKRMQELVKANLKVVREELPRAEAI
                     ELFRKLGEAYKVEIIEGIDADTVSLYRQGDWVDLCRGPHVPSTGSLKAFKLTSIAGAY
                     WRGDSRNEQLQRIYGTAWANKKDLEAYLKRIEEAKLRDHRRLGPALDLFSLHPIAPGS
                     PFFHPKGTIIYNILIQYMRDLYKRYGYSEVVTPLIYKTDLWKTSGHYEAFHDDMFLMN
                     VHDGEYGVKPMNCPGHCYLFGSRKYSYRDLPIRFADFSRLHRFEPSGVLSGLTRVRSF
                     AQDDAHIYCTPEQLDNELEQFVAMTKEVYKVFGFDRIEVTLQTRPEKFLGRIELWDAA
                     EAGLRRCLERSGFSVEVLPGEGAFYGPKIGFDFRDVLERSWTLATVQIDCAMPERFGL
                     KYVTSDGTEAAPVMLHRAVLGSIERFIAILLEHCAGAFPLWLAPVQLRVLTVTDDHKE
                     YAGKVVDQLREATWRVEFDGRNEKLGYKIREAQLAKVPYAIIIGEKEVAAETVAPRRR
                     GGENLPPLSVPEFLRNLHAEVAQEMGEA"
BASE COUNT         1387 a         1935 c         1759 g         1424 t
ORIGIN      
        1 cgatgatgat ctcatctccg gtctggggat ttctgccttt acgcggacgt ttagtgttga
       61 cgacaaaatt accgaaaccg gaaatcttta ctttatcgcc tttctctaaa gagcccttga
      121 tgatatcgaa ggtcgcttcg acgacatcgg tggcatcctt cttcgaaaaa cccactttct
      181 cataaatgcg cgtgatgatt tccgctttag tcatgttcca cctcctcgat ctctctgggg
      241 tcgcggcacc gctaactccg gcgttcggcg ccaaattcct tgcctatccg atcgactaca
      301 ctttggtgca atgggttgac ctcgctatcg gtgagggttc ggtcctcggc gcgataggaa
      361 atattgtacg ctaaactttt tttaccgtcc gggatcggtg cgccgagata ctgatcgaac
      421 acctgaacat gctcgattaa gggctcaccg agatggtcga gccaatcgat gattcgatac
      481 gctgcaaaat cgcgatccac gacgatggca aagtctcgtt cgatggccgg gaactttgcc
      541 agcggtcgaa ctcccatgtg ccgcggagca tactcaagca atttctcgaa gtcaagttca
      601 aatacataaa cttgcggcac ttccagtcgt tcacagacat cgggatggag ttcgccaagc
      661 aggcccatgg gacaactttt gtaggtccat tgggcggccc ggccaggatg taataggtca
      721 gacgccaaag cgttccaagc caatccatcg cgcacaccga acagctctag caagccttct
      781 atcgcgcctt tacagtgaaa aaaatccagc ttcggctcgt ccttgcgacg cagaccgtga
      841 accgccctcg ccccgtgcat gagcccgccg acgcagagac gctcttcgta cccaccgttc
      901 ggattgcagc ggaacacttt cccgagatgg taaacatgca ggctcgccgc cttttgcgct
      961 aaattgaccc ggagagtatc gatcagccca ggcaataggc tccggcgcag ggcgctattt
     1021 tccttggcca atggattgac caacggcacc gcttctccgg agccaagcca taaaccaggg
     1081 aacaaaccat tgatggcctc ggaggtgaat ggcagagtga ccgcctcgac gaaaccttca
     1141 gcggcaaaaa aattgcgcag cttacgttca ctggcgaggc gaaagtccgg cgcaccgcca
     1201 gtgggccgga gcgacggtaa cgtgatgggg atattgtcat aaccctgcag acgcgccaat
     1261 tcttcgatga gatcgatctc gcgcgtcaaa tctggccggc gcgtcggaat gacgacagtt
     1321 aactgctgag cttgccgggt agttttgaga ccgatggaac ggagcaactt ctcagcttgc
     1381 cggagctcca tcggcacgcc caatattcgc gatatgcgtt gttcgcgcag gcaaaccgtc
     1441 accggatcaa ctttttctgg gtaacaatcg gcggcgcccg gaagcggccg gcctccgcca
     1501 ttttcaccaa tcaactgcac cgcgcgatcc agtgcccgca gggttccttc ggggtcgacg
     1561 ccgcgttcaa atcggtacga cgcctcgcta tgcaaggcca agcgtttcgc cgtgcgccga
     1621 attgtcgctg gagcgaagtt ggcgctctcg agcaagagcg acgttgtcgc cgcgcttact
     1681 tcggaattca taccgcccat cacaccggcc agcgccactg gagtatcgcc gtcgcagatc
     1741 agcaaatcgt cgtgggcgag ctcccgctcg acgccgtcaa gggtggtgaa tttacggtgc
     1801 tcgcgagcgc tccgcacgac gatccgcttg gccggcaatt gcgctaaatc gaaggcgtga
     1861 agcggctgac cggtctcgat catgacatag ttggtcgcat cgacgacgtt attgatggcg
     1921 cggatgccgc aggattcgag gcggaaccgt agcaacggag tcgacctaac gattttgaca
     1981 tcgtcgacga tgcgcgccga gtagcgggcg cactggctga catcctcgat gttgagcgcc
     2041 acgtgctcat gcatacgcgg atctcgcttg ctcgccggca ccgggggaaa tttcacacgg
     2101 gttccggtca gcgcacaaac ttcctgggca atgcccaaca ggcttaaaca atcgccgcgg
     2161 ttgggcgtca cagcgatttc aaacaaccaa tcctgccgat ggccctccgg ctcctcgagc
     2221 ggcgttaacg actcaacctc caatcccgcg ctggtgagca acctggaaag ttcgccggcg
     2281 gtgccgccga attcgacgaa ttccttcagc caattgaggg taaatttcat aaaattggcg
     2341 taccggagtg ttgggatatt ggagtgatag gttgcgacaa cgtcacgcca acggcccagc
     2401 gctccatcat tccattccgt ttagctgaac tgccgtaaaa atcgcaagtc attctgaaaa
     2461 aacaatcgga tgtcgtcgat gccatacttc agcatggcga tgcgctcaac ccccatgccg
     2521 aaggcgaagc cggtaaattc ttcggggtcg tagccgacga atttgaaaac cgccggatcg
     2581 atcatgcccg cgccgaggat ttccaaccag ccgctggctt tacaaacccg acagctctgg
     2641 ccgttgcgca aggtgccgcc accgccgcaa atgacgcatt ggatatcgat ctcggcgctc
     2701 ggctcggtga aaggaaagaa actcgggcgg aaacgcacgc cagcatcgcg gttgaaaatc
     2761 tgccgcagca catgggtcaa cacgcccttc aggtcggcga aagcaatatg gctatcgacc
     2821 atgaagcctt cgacctgatg aaacatcggc gaatgggtcg cgtcgtcatc gtgacgatac
     2881 accgcgcccg gcacgatgat ttgcagcggc ggcttacggc tttccatggt gcgaatctgc
     2941 accggcgagg tgtgggttct caataaaccg ccgccgtcga cgaaaaacgt gtcctgcatg
     3001 tcgcgcgccg gatgatcttt gggaatattg agcgcttcga agttgtggta atcgtcctcg
     3061 atatcgggac cgcgggcgat ttcgaaaccc atggccgaaa agatctcgac gatctcgtcg
     3121 atcaccagag tcagcggatg gctatggcca cgttctagac gggtgcccgg cagcgagata
     3181 tcgatttttt cttcggcgaa agccttggcc ctggcttgcc ggttgacgac tgccaaccgc
     3241 gcatcgagct cctgctcgag agcgcggcgc agttgattga gccgttcgcc catacgcggc
     3301 cgctcttccg ccggcagttc tttcaaacca cgcaacagca gcgtaatcga accgctgcgg
     3361 cccaacgctt cgatgcgcag ctgttcgagc tccttgatgg attcggcttg ctggatgcgc
     3421 tccagaacct ctccgcggag acgctccagg gaatcggtca tgggactgaa ctgaaatagc
     3481 ggctactggc taaggtgagc tttagcggtt tccgccaagc ggccgaatcc cggcatatcc
     3541 gccaccgcga tttccgcaag aattttgcga tcgatctcga cattggcctt cttcaaacca
     3601 ttaatcactt gattatatga cataccgttg agcttggccg ccgcgcctat gcggatgatc
     3661 cacaggccgc gaaatgtccg tttttttacg cgccggtcgc ggtacgcaaa taccagcccc
     3721 cgttcgactg tttccaccgc ggagcggtac aggcgacccc ggccgccgac gtaccccttg
     3781 gcgagcttca taattttctt atgacgctgt ctagttttgg ttccaccctt ggctctaggc
     3841 actttaaaaa ctcctctcga atgtagattt caaccgccgg tgacctatgg ctggcgggtc
     3901 agacaaacgg ctacagatcg ttgaaggcta gttgaaggct atttattatt tataaggaat
     3961 caactgacga atgttcttcg cttccaccgc cgccaccatg gttcccttgc gcaagttgcg
     4021 tttgcgcttt ttacccttgg acgacaggat atgacgcttg taagccttgt tgcgttttac
     4081 cttgccgctg ccggtgacac ggaagcgctt cgctgctccg cgattggttt tgattttcgg
     4141 cacgtttcac tccctctcgt ttatttcaaa ctttgatcac ttcggcgtca atagcatcgc
     4201 catattgcgc ccttcaagac gcggctgaat atcgaccttg gcgacatctt gaaccaactc
     4261 gacaactccc gtaagcagat gccggcccag ttcaggatgg gtgatctcgc ggccgcgaaa
     4321 gaacaccgac actttgaccc gctggccttc atcgataaat ttgcgaatgt tcttcacctt
     4381 atgctcgatg tcatgggcgt cggtgcgcga gcccatcttg acttccttga tctcgaccac
     4441 cgtcttcttc ggtccctgct gctttttctt ttgctcgtaa cggaacttgc cgtagtccat
     4501 catgcggcat accggcggct gagcgtccgg cgcgacttcg accaagtcaa gatccagagc
     4561 ttccacctgc ttcagcgcct cgtgcagcgg catcactcca agctgttcgc ccttggaacc
     4621 gatgatgcgg acttcacggg cacgaatttg atgattgatt cgcgcttccc tagcgataag
     4681 acacctccgt ggtttctaac ggcagcatga tcctctattt ctatgcctct cccatttctt
     4741 gtgcaacctc ggcatggaga ttcctgagga actccggcac cgacaacggc ggaagatttt
     4801 ccccaccgcg ccgccgcggc gccacggtct cggccgccac ttccttctcg ccgataatga
     4861 tcgcgtaggg gactttggcc aactgcgcct cgcggatctt gtagccgagc ttttcgttgc
     4921 gaccgtcgaa ctcgacgcgc caggtcgcct cgcgcaactg atcgacgacc ttgccagcgt
     4981 actctttatg gtcgtcggtc acggtcagga ctctgagctg caccggggcc aaccacaatg
     5041 ggaaagcccc ggcgcagtgc tccaagagaa ttgcgatgaa gcgctcgatg gagcccaaca
     5101 ccgcccgatg cagcatcacc ggcgccgcct ccgtgccgtc ggaggtgaca tatttaagac
     5161 cgaaccgctc gggcatggca caatcgatct gcaccgtggc cagcgtccaa gagcgctcca
     5221 agacatcgcg aaaatcgaag ccgattttcg ggccgtagaa tgccccctcg cccggcagga
     5281 cctcgaccga aaaacccgag cgctcgagac agcgccgaag tccggcctcg gcggcgtccc
     5341 acaactcaat gcggccaaga aatttctcag ggcgggtttg cagcgttact tcgatgcggt
     5401 cgaagccgaa tactttgtag acttctttgg tcatcgccac gaactgctcg agctcgttgt
     5461 ccagctgctc cggcgtacag taaatatgcg catcgtcctg agcaaaagaa cgcacccggg
     5521 tcaaacccga aagcacgccg gacggctcga agcgatgcaa ccggctaaaa tcggcgaagc
     5581 gaatcggcag atcgcggtag gagtatttgc gcgagccaaa caggtagcaa tggccgggac
     5641 aattcatcgg cttgacgccg tactcgccgt cgtgaacgtt catcaggaac atgtcgtcgt
     5701 gaaaggcctc gtagtggccg gaggtcttcc atagatcggt tttatagatc agcggcgtga
     5761 ccacctcgga gtaaccgtag cgcttgtaca gatcgcgcat gtactggatc aggatattat
     5821 aaatgatcgt gcccttggga tgaaaaaacg gcgatcccgg cgcgatcgga tggagggaaa
     5881 acaaatccaa cgccggtcca aggcgccgat gatcgcgcag cttggcctct tcgatccgct
     5941 ttaagtaagc ttctaaatct tttttgttgg cccatgcagt gccgtagatt cgctgcagtt
     6001 gctcgttgcg cgaatcgccg cgccaataag cccccgcgat gctggtcagc ttgaacgcct
     6061 tgagcgagcc ggtggatggc acgtgggggc cgcggcagag atcgacccaa tcgccctggc
     6121 ggtagagcga taccgtgtcg gcgtcgatgc cttcgatgat ctcgaccttg taagcttcgc
     6181 ccagcttgcg gaacagctcg atggcctcgg cgcgcggcag ctcttcacgc accactttca
     6241 aattcgcctt gaccaattcc tgcatgcgct tctcgatccg ctcgagatca tcgggcatga
     6301 acggctgatc ccgtttgaag tcgtaataga aaccatcgtc gatggtcggg ccgatggtta
     6361 cctgggtgcc cgggaataag ctctgcaccg cttgcgccat caaatgcgcg ctcgaatggc
     6421 gcaatacatc aagccctgcg gcgctctcag gcgaaatcca ttccacggtg cagtctttat
     6481 cgatgatccg attcaaatct accag
//
LOCUS       DASQKS010000304         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2691375, whole genome shotgun sequence.
ACCESSION   DASQKS010000304 DASQKS010000000
VERSION     DASQKS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691375"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VE170_07915"
     CDS             <1..911
                     /locus_tag="VE170_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15399.1"
                     /translation="LKTTPKIELLRPFAAPATLSLLVTRADGRPVTQGVVAIVLDAPK
                     PGTLLSTDFPLVEGSRLLEMVLPLRRGRAEWKYLFPIRGNYRLSVNFVDADGQGTSKD
                     FPIPVLEERQKWLWLGLFCAGLFLIGFSAGRIFTTPARGGMITALLALSLAVVANVGG
                     HDPPPTQAVSNPQSALEIDGAVVGKLTRLRWRPSDAAVVNRQLSLAITHLEKRKIMFA
                     VDRVPVASEFGLKFHYPDGAEYRVNAVAESPGQAPVRAEQLVAVTGIEPAMMAQIPAL
                     LFFLSVISLGLGAGRFSFRYHHRAPR"
     gene            complement(947..1807)
                     /locus_tag="VE170_07920"
     CDS             complement(947..1807)
                     /locus_tag="VE170_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HYJ15400.1"
                     /translation="MPPSTLLFGATSILGYSIAKLFPDTVLPFVTRANRAKSLRQWPV
                     LNLEDPAWPATVFAMHQPDLFIQCHAVCDVPRCEVAPDWAREVNIVYLKRVLDALPDK
                     TRLVYVSSDHVFGGDGVYDEESPPCPISVYGKTRVEAEAMVLARPGSLVIRVGLAIGA
                     SPNGRTGHWDWLRYRIGKNLPVTIVDDEYRSVVWAHDLALRVMRMAESREAGIRHVAA
                     TRAISRIALANHLLTIFGEPASYERESRHQRSAPHLGRVELASIHRGELYEPLRCVLD
                     GPNVLGRFYD"
     gene            complement(1819..2055)
                     /locus_tag="VE170_07925"
     CDS             complement(1819..2055)
                     /locus_tag="VE170_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15401.1"
                     /translation="MLEFLSWVASRPRTYDEAMEAWQSHCPRQTIWEDAIIEGLIEIN
                     RQPNDHVVILTARGEAILNAKNHDRKNHPVKASP"
     gene            complement(2110..3081)
                     /locus_tag="VE170_07930"
     CDS             complement(2110..3081)
                     /locus_tag="VE170_07930"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15402.1"
                     /translation="MLKFKFPALSLFVVLLLASPGSAQQLVRVGYSGTGVAKSLHKTI
                     ERAGLWKKRGLDVRLIYFTSGATMAQAMVGGDLDLADSDVPAMLNAVSVGALDGKLIS
                     VYINRFPFAFVVRNDIKTPEDLKGKQVAISRFGSSSDVTTRMLLRQFNLDPEKDVRII
                     QATGTTRVPAMIAGQLHGTLLGTYDVPIITESGCCRVMVDMLELPIDYARFGQVVPTQ
                     FLRSKRELLSKFAEGLIEGIYVFKTNKELAMSVLRAEGIKGPEYSYPRVAAAMRERPI
                     PEAKGVQAVLDSVKSPKSKLTLAKEVIDGSLIEEIDKSGYIAKLYGK"
     gene            complement(3165..>3407)
                     /locus_tag="VE170_07935"
     CDS             complement(3165..>3407)
                     /locus_tag="VE170_07935"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15403.1"
                     /translation="NDVTWAMCTRSDPQADTDFIRRAWSGPLDPVIPKDKKGFSSRAV
                     IDATRPYEWIKDYPKVSGSSAEMKQLATKKYGKFFE"
BASE COUNT          668 a          950 c         1004 g          785 t
ORIGIN      
        1 ttcttaagac gacgcctaaa atagaactgc tgcggccgtt tgccgccccg gcgactcttt
       61 ctctgctggt gacccgtgcg gacgggcggc cggtgaccca gggcgtggtc gcgatcgtcc
      121 ttgacgcgcc aaagcccggc accttactat ccaccgattt tcccttggtc gaaggcagcc
      181 gcttgctcga gatggtcttg ccgctgcgcc ggggacgcgc cgagtggaaa tacctttttc
      241 cgatccgcgg caattaccgg ctgtcggtca atttcgtcga tgccgacggt caagggacga
      301 gcaaggattt tccaattcct gtgctggagg agcgccagaa gtggctttgg ctcgggctct
      361 tttgcgccgg attgtttcta atcggtttta gcgccgggag gatatttacc acaccggccc
      421 ggggtgggat gataacggcg ttgctggcgc tgtccctagc cgtcgtcgct aacgttggcg
      481 gacatgaccc gccgccgaca caagctgttt cgaacccgca aagcgctctg gaaattgatg
      541 gcgcggtggt cggtaagtta acccgtctgc gctggcgtcc gagcgatgcc gctgtggtca
      601 atagacaatt gtcgttggcg atcactcatc tggaaaaacg caaaatcatg tttgccgtcg
      661 acagggtgcc agtggcgagt gagttcggct tgaagtttca ctatcccgat ggcgcggagt
      721 atcgcgtgaa tgccgtcgcc gagtcccccg gacaagcgcc ggttcgcgcc gaacaattgg
      781 tggcggttac cggtatcgag ccggcgatga tggcgcaaat tcccgcgctg ttgttttttc
      841 tgtcggtgat ctcgcttggt cttggcgccg gacgatttag ctttcgctat catcatcgcg
      901 cgccacgtta attttttagg cagcctattg gttctttggg acgctatcaa tcgtaaaatc
      961 ggcctaggac attggggccg tccaaaacgc accggagcgg ttcgtacaac tcgcctctgt
     1021 ggatactcgc caattcgacg cggcccagat ggggcgctga gcgttggtgg cggctctccc
     1081 tttcatagct tgctggttcg ccaaaaattg tcagcagatg gttcgccagt gcgatgcgcg
     1141 agatcgcccg agtagccgcg acatggcgga ttccggcttc gcgactttca gccatgcgca
     1201 tcacgcgcaa agccaaatca tgggcccaga ccaccgagcg atattcgtcg tcgactatcg
     1261 tcaccggcag attcttgccg atgcggtagc gtagccagtc ccaatggccg gtgcggccgt
     1321 tgggtgaggc gccgatcgcc agaccgacgc ggatcaccaa agagccaggc cgggccagaa
     1381 ccattgcttc cgcctccacc cgcgttttgc cgtaaacact gatcggacag ggcggcgact
     1441 cttcatcata aacaccgtcg ccgccaaaga cgtgatcgga ggagacgtag acgagccgcg
     1501 ttttgtccgg caatgcgtcg agcacccgtt tgagataaac aatgttcact tcgcgcgccc
     1561 agtcgggtgc cacctcgcag cgcggcacat cgcacaccgc atggcactgg ataaataaat
     1621 cgggttggtg cattgcaaaa accgtcgccg gccacgccgg atcttcgaga ttcaacaccg
     1681 gccattggcg aagcgatttc gctcgattgg cgcgggtgac aaacggcaag accgtgtcgg
     1741 ggaaaagttt ggcgatgctg tagccgagga tcgacgtggc gccgaaaagg agagtggagg
     1801 ggggcattga gcctagactt atggagatgc tttaaccgga tgattcttcc ggtcgtgatt
     1861 tttcgcgttg aggattgctt cgccccgcgc tgtcagaatc accacgtgat cgttaggttg
     1921 ccgattgatc tcgatcagcc cctcgatgat cgcgtcctcc caaatcgtct gcctcgggca
     1981 gtggctctgc caggcttcca tggcttcgtc ataggtgcgc gggcggctcg ctacccagga
     2041 gagaaactct agcattagga gatcctgcga ttctgtcatg gcagtatcag gttgaggaaa
     2101 agctaaattt tatttcccat acaactttgc aatatacccg cttttgtcga tttcttcgat
     2161 caggcttccg tctatcacct cttttgcaag ggtcagtttt gacttcggcg atttgaccga
     2221 atccaggacc gcttgcacgc ctttggcttc gggaatcggg cgttcgcgca tggcggcggc
     2281 gactctggga tagctgtatt cggggccttt gattccttcg gcgcggagca ccgacatggc
     2341 cagctcttta ttggttttga aaacgtaaat gccttcgatc aatccttcgg caaatttgct
     2401 caacagttca cgtttgctgc gcaagaattg cgttggtacg acctggccga agcgggcgta
     2461 gtcaatgggc agttccaaca tgtcgaccat gacgcggcag cagccggatt cggtgataat
     2521 cggcacgtcg taggtgccga gcaacgtgcc gtgcagttgg ccggcgatca ttgccggaac
     2581 gcgggtcgtt cccgtcgctt gaataatgcg cacgtctttt tccggatcga gattgaattg
     2641 ccgcaatagc atgcgcgttg tgacatcgga cgatgaaccg aatctgctga tggcaacttg
     2701 tttgcccttt aaatcttccg gcgtctttat atcgttgcgc acgacgaagg cgaagggaaa
     2761 gcgattgata tagacactga tcagcttgcc gtccaacgcg ccgacggaca cggcattgag
     2821 catggccggc acgtcagaat cggcaaggtc caggtcgccg ccgaccatcg cctgagccat
     2881 ggtcgcgccg ctggtaaaat aaatcagccg cacgtcgagg ccgcgcttct tccacagacc
     2941 ggcgcgctcg atggttttgt gcaggctctt ggccacgccg gtgccgctgt agccgacacg
     3001 gacgagttgc tgggcggagc cgggtgatgc gagtaacagc actacgaata ggctcagcgc
     3061 ggggaatttg aatttcagca tgccggttcc ttttcgtaag agcttacgga gtaagtaata
     3121 aggattctgg acctatagcc ccaagcctaa ctatctcccc ctcactattc gaaaaatttc
     3181 ccatactttt tcgttgccaa ctgtttcatc tcggcgctcg aaccagagac tttcgggtaa
     3241 tccttaatcc actcgtaggg ccgggtggca tcgatgacgg cgcggctgct gaaacctttc
     3301 ttatccttcg ggatcaccgg gtcgaggggg ccgctccagg cacggcggat gaagtcggtg
     3361 tcggcttggg gatcggagcg tgtgcacatc gcccaggtaa cgtcatt
//
LOCUS       DASQKS010000305         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3098720, whole genome shotgun sequence.
ACCESSION   DASQKS010000305 DASQKS010000000
VERSION     DASQKS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3098720"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VE170_07940"
     CDS             <1..426
                     /locus_tag="VE170_07940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYJ15404.1"
                     /translation="NLPVIIEESIRELDHGDLEGLTFNEIKQHHGEFLARWRSEPAEL
                     QVPGGERLADVAERAWLGLHRIVMRHQPSDTIVVVSHNFPIVGIVCRITGTDLNEYRK
                     FHLDPCGISRLNRNREGSWRVTHINGDMLENHRGRALPN"
     gene            complement(466..2004)
                     /locus_tag="VE170_07945"
     CDS             complement(466..2004)
                     /locus_tag="VE170_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HYJ15405.1"
                     /translation="MADSASNPPWFVRKDLDGFFGLMIDNLIQLILIVGLCRELIRLP
                     DSYIFGKILPGAAISILVGNFFYAWQARRLARESGREDVTALPYGINTVSLFAFVFFI
                     MLPIVQETKDPVWAWKVGLVACFLNGVIEILGAFVAEAVRRVTPRAALLSALSGIAIT
                     FIAMDFTFRIFAQPLVALVPMGLIFVAYFSHQKLPLGLPGGMVAITVGTILGWTLGNM
                     KGEALAVNWLLSLPAFAGDSLWEALMHPAFLSYFAVIFPMGIFNVVGSLQNIESAEAA
                     GDRYPTFSSLMANGIGSVVAALFGSVFPTTIYIGHPGWKRLGARSGYSALNGVFIAFL
                     CFTGTIQAVLALIPLEAGIGILLYIGIIIVAQSFQETPKEHAPAVALGLVPALAGWGL
                     MMVETGLRAAGKSLYEVGLGKFSHESVAIHGMISLERGFIFTSMILAAIGVALIERQF
                     VRAGLWSVAAGLLSAFGIIHAYDLTAGGITSRFGVMAAPEFFTAYLALAVIFFVIGLI
                     AKRQ"
     gene            2115..>2771
                     /gene="lipB"
                     /locus_tag="VE170_07950"
     CDS             2115..>2771
                     /gene="lipB"
                     /locus_tag="VE170_07950"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYJ15406.1"
                     /translation="MDDKNGQQMSTLLFKNLGQLDYLAAVQLQERLLSLKQQESLADV
                     LLLVEHPQVYTLGRGGNPANLLAPGEIPVHRTSRGGDVTYHGPGQLVVYPIVDLRSKQ
                     RKDVHRYVRNLEQSAILTLADCGITTTRRPPYTGIWIDDKKIAAIGVAVRRAITYHGL
                     ALNVNTDLAYFQRIIPCGLAWAEVTSMKKELGIEQNVSNVRVRFLHHFAETFGHRDVV
                     E"
BASE COUNT          684 a          845 c          750 g          492 t
ORIGIN      
        1 aacctcccgg ttataatcga ggaaagcatt cgtgagctcg accatggcga cttggaagga
       61 ctgacgttca acgagatcaa acagcaccac ggcgaatttc tggcgcgctg gcgcagcgag
      121 ccggcggaac ttcaagtgcc cggcggcgag cgcctggccg atgtcgctga acgcgcctgg
      181 cttgggctcc atcggatcgt catgcgccat cagccttcag ataccatcgt cgtcgtcagc
      241 cacaattttc cgatcgtcgg catcgtctgc cggatcaccg gaaccgacct gaacgaatac
      301 cgcaaatttc atttggaccc ctgcggcata agccggctta accgcaaccg tgagggctcc
      361 tggcgggtca cccatatcaa cggcgacatg cttgagaacc atcgcggccg agcgctgccc
      421 aactgacgaa gttctatgcc gcacagcaat ccgcggtccc gcctgtcatt ggcgcttggc
      481 gatcaaaccg ataacgaaga aaattaccgc cagggcgagg taggcggtaa aaaattccgg
      541 cgccgccatc acgccaaacc ggctggtaat ccctccggcg gtaaggtcgt aggcatggat
      601 gatgccgaag gccgataaca accccgccgc gaccgaccac agcccggcgc ggacaaactg
      661 ccgctcgatc agcgccacgc cgatggcggc aagaatcatt gaagtgaaaa tgaaaccgcg
      721 ctccagcgaa atcatgccat ggatcgccac gctttcgtga ctgaatttgc ccaagccgac
      781 ttcgtagagc gactttcccg cggcgcgcaa accggtttcg accatcatca atccccaacc
      841 tgccaaggcc ggcaccagtc ccagcgccac cgcaggcgca tgttctttcg gcgtttcctg
      901 aaacgactgg gcgacgatga tgatgccgat gtagagcaga ataccgatgc cagcctccag
      961 agggatgaga gcgagcacgg cttgaatcgt accggtaaaa cagagaaaag cgatgaacac
     1021 gccgttgagt gccgaatagc cggaacgggc gcccaaccgc ttccaaccgg gatggccgat
     1081 gtagatggtc gtcggaaaca cgctgccgaa caacgccgcc accacggagc caataccatt
     1141 ggccatcagc gaggaaaatg tcgggtagcg gtcgccggcc gcttctgcgc tttcgatatt
     1201 ctgtagcgaa ccgaccacgt tgaaaatccc catgggaaaa attaccgcaa agtaactcag
     1261 aaacgccgga tgcatgagcg cttcccacaa cgaatcgccg gcaaacgccg gcagcgacaa
     1321 cagccagttt accgctaacg cctcaccttt catgtttccc aacgtccaac cgaggatcgt
     1381 gccgacggtg atggcgacca tgccgccggg caggccgagg ggcaatttct gatgggaaaa
     1441 gtaagcgacg aaaatcaacc ccatcggcac cagcgcgacc agcggttgcg cgaaaattct
     1501 gaaggtgaaa tccatcgcga tgaaagtaat cgcgataccg gacaacgccg acagcaacgc
     1561 cgctcgcggc gtcacccggc gcaccgcttc ggcgacaaac gcgccgagga tttcaatcac
     1621 gccgttgaga aagcacgcga ccagacccac cttccacgcc cacaccggat ctttagtctc
     1681 ttgcacgatc ggcagcatga tgaaaaagac aaaggcaaac aagcttacgg tgttgatgcc
     1741 gtaaggcagc gctgtgacat cttcgcgccc gctctcccgc gccagccggc gcgcctgcca
     1801 cgcgtagaaa aaattgccga cgagaatcga gattgccgct cccggcagga tcttgccgaa
     1861 tatatagctg tcgggcagcc ggatcagctc acggcacaag ccgacgataa gaatgagttg
     1921 aatcaaattg tcgatcatca agccgaagaa accgtcaaga tctttacgga caaaccaggg
     1981 tggattggaa gcagaatcag ccataagcga aaaattctcc gctaaagttt cgcagacttt
     2041 agccgtcgct gctcggtggt gtcaaatcgt atccacacaa ttcgactggg caactcaact
     2101 tgaatccccg cctgatggac gataagaatg ggcaacagat gtcgactttg ctgttcaaaa
     2161 acctcggaca gttggactac ctggccgccg ttcaattgca ggaacggttg ctctcgttga
     2221 agcagcaaga atcactcgcg gacgtactcc tgctggttga acatccccag gtatacactc
     2281 tcggtcgcgg cggcaacccg gcaaacctgc tggcgccggg ggaaatcccc gtgcaccgca
     2341 ccagccgcgg cggcgatgtg acgtaccatg gccccggtca gttagtcgtc tatccgatcg
     2401 tcgatctgcg ttcaaaacaa cgcaaagacg tgcaccgtta tgtgcgtaac ctggagcaaa
     2461 gcgcgatcct aacgctcgcc gactgtggca tcacgaccac ccgccgcccg ccgtacacag
     2521 gcatctggat cgacgacaaa aagatcgccg ccatcggcgt cgccgtgcgc cgcgcgatta
     2581 cctaccatgg gttggcgttg aatgtgaata ctgatctagc ttactttcag cgcatcattc
     2641 cgtgcggctt ggcttgggct gaagtgacgt cgatgaaaaa agaactcggc attgagcaaa
     2701 acgtcagcaa tgttcgagtg cgctttctcc atcacttcgc cgagaccttc gggcatcgtg
     2761 atgtcgtcga a
//
LOCUS       DASQKS010000306         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1012711, whole genome shotgun sequence.
ACCESSION   DASQKS010000306 DASQKS010000000
VERSION     DASQKS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012711"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            570..1235
                     /locus_tag="VE170_07955"
     CDS             570..1235
                     /locus_tag="VE170_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15407.1"
                     /translation="MKTPSTPHEERTRGISRESMQRLQQRHFHKLLIHVWNRSAFYRD
                     YYSSHGVREKSLDDMSIGDLPLLPKKLLIANFDRAVTDPRLRRKELEEWFESHRDPNE
                     TFCQDMIVIHGSGTSGDIGIYAYDRRAWTIADTTMAGRLPLPENYPAGKTKIAFYVAA
                     NGHFATVSMASSMPKSVYDTLILSLMDSVEQTLTQLNAFQPHRLTGYSSSVAQLAELA
                     LDG"
     gene            1259..3907
                     /locus_tag="VE170_07960"
     CDS             1259..3907
                     /locus_tag="VE170_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYJ15408.1"
                     /translation="MGGDKLSENMERKIRAAWNVPLYVIYAASESKYIAIKMPERDQM
                     MVMDDLNIVEALDENNREVGAQQEGRVVLTNLYNYILPMLRYELGDYVVRGAELPDLP
                     FTTLRDIRGRVNDALPIVLSSGQLDSIHPVVLTTFHVPTIEKFQFISASPEHVRIDYV
                     AASNIDPSVRREFERMLQEKGGARTTLDCRHVSSIANDPVTGKLRLVKFEGRTVAPSP
                     PVTRTDVPAAVMHPVIVPRSVRIQPDASFAPFKKTDIEQTISARFEQQAEKYADRLAV
                     KHRSLSLSYAELNRAANRLAHAIIARRGANQESVALVLHHGISAIIAILAILKAGKCY
                     VPLDSGFPAARLTGILEEADVSLLITDDRNVSSALPFAREPGAILNIDTLDAGLSLDN
                     PHLAISPDSLAYLFFTSGSTGRPKGVAHSHRNGLHQIMTYTNGLMLSPQDRMTQLHSH
                     GFSASRLDIFGALLNGAALLPLLAAEEGMSGLARRLIYEEMTLFHWVPTAFRHFADSL
                     PVSSDGMDMFPTLRSIVLGSEPLTARDVELFKRHFSSDCVLVNRFGSTETGNMTWYFM
                     GKQTNVPGGIAPVGYPVEDAEMLLLDETDNDVGINQIGEIAVKSAYLPAGYWRRPDLT
                     AGVFSPAPKAPGKTIYRTGDLGRRMPDGCLVHLGRMDFQVKIRGYRVELGEIESALAN
                     HSGIASAVVTARHDNLGDMSLAAYFVAATSPPPATSTLRHYLEARLPVYMVPAAFMSL
                     KALPLTPNGKVDRKALPEPDPVRADSEAVATTGPRTNVEEILAGIWSRVLGVSSVNNH
                     DNFFDLGGNSLSAMLIISRTVSALNVVVSIGEFFEIPTVAHMAELVGSASLKNYDDPE
                     LAEMLDLVESLSEEQANQLLKKEPEE"
     gene            complement(4070..4417)
                     /locus_tag="VE170_07965"
     CDS             complement(4070..4417)
                     /locus_tag="VE170_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15409.1"
                     /translation="MRDASLPVTLIIVGSAWLLWYYRLFPDIDWIIAVGLIVGGIAVL
                     YLDGITKKSIVAGPFLIASGIAWALHDRWHVTWIVLIPTLLIMLGVLMLIGRGANIPE
                     RRLGTGEIKGGSE"
     gene            complement(4540..4734)
                     /locus_tag="VE170_07970"
     CDS             complement(4540..4734)
                     /locus_tag="VE170_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15410.1"
                     /translation="MKQTWLPDERELQPVGVNASISAVIESHRILAWIGLREISIQLA
                     TRSPWQASWTKLAPQKGFFA"
     gene            4847..>5032
                     /locus_tag="VE170_07975"
     CDS             4847..>5032
                     /locus_tag="VE170_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15411.1"
                     /translation="MKRNKTEWRWFRQISLGAVLIDLFIELFMGSVALPQTSFFQGKT
                     VTILESSAPGGVGSMRTK"
BASE COUNT         1170 a         1419 c         1316 g         1127 t
ORIGIN      
        1 gccattcctc ggcaacaagc agggaccgac caagaacgaa gtgctgtcgc gcgccgtcga
       61 gcatgcggcc atgaaagacg ctgccgcaga gcttaccacc cggcccgatg cacccttcga
      121 cgacatcccg ctaaacaaag agacgaccaa aaccgcccac gcccattgaa aatcgacgcc
      181 catgcctgtg ccggcacggg gcagtaaagc aggaatccgg gtcagatctt tacaggacgt
      241 taaaaactcg cttttcattt caaagccgct taccatccag ataatacgtt ctaaccatcg
      301 ctatggtcgc gatcctgcca ctaccgggcg catctgagtt ttcaacagct tgtttatctc
      361 tgcgacatcc aaccgacagc gcaaccaaaa ctaccatgcc ctaactgtaa aaaagattga
      421 tcctatactt tgtacagtac tcaaactcgt gaattagtta ctcgccgggt cggcgctatc
      481 gcacccgttt gcgaaagact ctggttatat ttcctattcg taaaatgtcg accggtccca
      541 tcgcttcggg agtatcttga gggcgggata tgaagacacc atcgacccct catgaggagc
      601 gaacgagggg gatatcgcgc gaatcgatgc agcggctaca gcagcgccat ttccacaaat
      661 tgctcatcca tgtttggaac cgatccgcgt tttatcggga ctactacagc agccacggag
      721 ttcgtgaaaa atctcttgac gacatgtcga tcggcgatct gccgctactg ccaaaaaagt
      781 tgctcattgc caatttcgac cgggctgtta ccgatccccg tcttcggcgg aaagagctcg
      841 aagaatggtt tgagagtcat cgtgacccaa acgaaacttt ttgtcaggat atgatcgtta
      901 ttcatggttc ggggacttct ggagatatcg gcatctacgc ttatgatcga agggcctgga
      961 ccatagccga cacgacgatg gcagggcgtt taccattgcc ggaaaactat ccggcaggca
     1021 aaaccaaaat tgccttctat gttgcggcaa atggacattt tgcgacggta tcgatggcca
     1081 gctcaatgcc caagagtgtc tacgatacgt tgattctgtc ccttatggac tcggtggagc
     1141 aaacgctcac ccagctgaac gcgtttcaac cgcatcgtct caccggctat tccagcagcg
     1201 tggcccaatt ggccgaactc gcccttgacg gctgaatatc tcgccacaga gaatatttgt
     1261 gggtggggac aagctgtcgg aaaatatgga aagaaagatt cgcgcagcgt ggaatgtacc
     1321 gctgtatgtg atctatgcgg catcggagtc gaaatacatt gccataaaga tgccggagcg
     1381 cgaccagatg atggtcatgg acgatctgaa tatcgtcgag gcgctcgatg aaaataatcg
     1441 ggaagtcggc gcgcaacagg aaggcagggt ggtccttacc aatctttaca actatatcct
     1501 gccgatgctg cggtacgagc ttggcgacta tgttgtacgg ggcgcggagc tgccggatct
     1561 tccctttaca accctgcgcg atatccgagg cagagtgaac gacgcgctgc ccattgttct
     1621 ttccagtggc cagctcgaca gcattcatcc cgtggttttg accactttcc acgtgccgac
     1681 catcgagaag tttcagttca tttcagcgag tccggagcat gtgcgcatcg actatgtcgc
     1741 cgcgagcaac atcgacccgt ccgtccggcg cgagttcgag cgaatgctgc aggaaaaagg
     1801 cggcgcgcga acgactctgg actgccggca tgtatcgtcc atcgccaacg accccgttac
     1861 cggcaagctc cgtttggtga agttcgaagg gcgcacggtt gcgccatctc ctccagtcac
     1921 acgcactgat gttccggcgg ctgtcatgca tccggtaatt gtcccgcgtt cggtgcgcat
     1981 ccaacctgat gcttcctttg cgccattcaa aaagaccgac atcgaacaaa cgatttcggc
     2041 acgtttcgag cagcaggccg aaaagtatgc agatcgcctg gccgtaaaac atcgcagtct
     2101 ttcgctgagt tatgccgagc tcaaccgcgc ggcaaatcgc ctcgcgcatg cgatcatcgc
     2161 acggcgcggc gcgaatcaag aatccgtagc gctggtcctg caccatggca tttcggctat
     2221 cattgcgatc ctggccatct tgaaggccgg caagtgctat gtcccactgg actccggctt
     2281 cccggctgca cgacttaccg gcatactcga agaggctgac gtatcgctgc tgattaccga
     2341 cgaccgcaat gtttcttcgg cgctgccatt tgcccgcgag cccggcgcca ttctgaatat
     2401 cgatacactt gatgctgggt tatccttaga caatccacac ctggccattt cacccgactc
     2461 tctggcctat ctcttcttta cctccggttc caccgggcgg cccaagggag tggcgcattc
     2521 tcatcgcaat gggctgcatc agatcatgac ctatacgaat ggcctgatgc tttctcccca
     2581 agaccgcatg acgcaacttc actctcatgg tttcagcgcg tcccgactgg acattttcgg
     2641 cgcattactc aatggcgctg cactactgcc gttattggcc gcagaagaag gaatgagcgg
     2701 actggcccgt cggctaatct acgaagaaat gacgctgttt cattgggtgc caacggcatt
     2761 tcgccatttt gccgacagtc ttcccgtctc ctccgacggt atggatatgt ttccgacgct
     2821 gcgttcaatc gtacttggca gcgagcctct cacggcgcgg gatgtggagc tttttaagcg
     2881 gcatttctct tccgactgcg tgctggtcaa ccgcttcggc tcgacggaga ccggcaatat
     2941 gacctggtat ttcatgggta aacaaaccaa cgtgccgggc gggatagcgc ccgttggcta
     3001 tcctgtcgag gatgccgaga tgctcttgct cgatgaaacc gataacgacg ttgggatcaa
     3061 tcaaattggc gaaatcgcag tcaagagcgc ctacctgccg gccggctatt ggcggcggcc
     3121 tgacttgacc gccggtgtgt tttcacccgc gccgaaagcc cccggcaaga ctatttatcg
     3181 caccggagat ttgggccgtc gcatgccgga tggctgcctc gtgcatctcg gacgtatgga
     3241 ctttcaagta aagattcgag gttatcgcgt cgagcttgga gaaattgaaa gcgcgctagc
     3301 caaccattcc ggtattgctt cagccgtggt gactgcccgg cacgacaatc tcggcgacat
     3361 gtctctggcg gcctactttg ttgctgccac ctcgccccca ccagccacga gtacattacg
     3421 ccactatttg gaagctcgct tgccggtata catggtacct gcggcattca tgagcctgaa
     3481 ggctctgccg ctcacgccga atggcaaggt ggatcgcaaa gcattaccgg aaccggatcc
     3541 tgtgcgggcc gacagcgaag cagttgccac taccggacct cgtactaacg tcgaggaaat
     3601 tctcgcaggc atctggagcc gcgtgcttgg agtcagctcc gtaaacaatc acgacaactt
     3661 tttcgatctg ggcggcaatt ccctctccgc catgctaatt atttctcgta cggtcagcgc
     3721 ccttaatgtt gtcgtctcca taggcgagtt cttcgaaata cccacggtcg cgcatatggc
     3781 tgagttagtc ggcagcgcta gtttgaaaaa ctatgacgac ccggaattgg cagaaatgtt
     3841 ggacctggtc gagtcgcttt ccgaggagca ggccaaccag cttctgaaaa aggaaccgga
     3901 agaataagcc gggatcactg gaaagcaatt tgaaaacggc aggtcagtaa atcggaaagt
     3961 catgccatag gtctctcgcg agagtaaaga caaccaagct tcataagccg acccgcgtga
     4021 agaaccccca tagtccaagc aggatcgctt agactcaatc cattcaattt tattcgctgc
     4081 cacctttgat ctcgcccgtg cccaatcgcc gctccgggat attcgcgccg cgcccgatca
     4141 gcatcaacac accgagcatg atcagcaggg tagggatcag cacgatccag gtcacatgcc
     4201 agcgatcgtg cagcgcccaa gcaatgccgc tggcgataag aaatggccca gcgacaatgg
     4261 atttcttggt aatcccgtcg aggtacaata ctgcaatgcc tccgacaatt agtcccacgg
     4321 cgatgatcca atctatgtcg ggaaacaacc ggtagtacca cagcaaccat gcgctgccga
     4381 cgataatcag ggtcaccggt aagctggcgt ctctcattaa ttttcctttt gtcgaggcgg
     4441 caaagaagat tgctgtggct tgtgctagtt agttgggtgt atatcgtaat atgagcggtc
     4501 acctagactt ggcgacttta ctgaagacgc aaaaatccgt caagcaaaga aacccttttg
     4561 cggcgcaagt ttggtccacg acgcttgcca cgggctgcgc gtcgccaact gaatggatat
     4621 ctcgcgcagt ccgatccacg ccagaatacg atgactttcg atcacggctg aaattgaagc
     4681 attcacgcca acaggctgca gctcgcgttc atccggcagc caagtttgct tcattgacag
     4741 aatttttcag aggtgttagt cgggcatcga gtcataaacg gttcaccgga cgaatcgcgg
     4801 caagggagtt gccgaaggtg ggaagtgtca tatcacggag gtgcgtatga aacgcaacaa
     4861 gaccgaatgg agatggtttc ggcaaatctc tcttggcgcg gtattgatcg atctcttcat
     4921 tgaactcttc atgggcagcg tcgctttgcc gcaaacttct ttctttcaag gcaagaccgt
     4981 gactatcctt gagagcagcg cgccgggcgg agtcggcagc atgcgcacca aa
//
LOCUS       DASQKS010000307         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_157010, whole genome shotgun sequence.
ACCESSION   DASQKS010000307 DASQKS010000000
VERSION     DASQKS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157010"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VE170_07980"
     CDS             complement(<1..274)
                     /locus_tag="VE170_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ15412.1"
                     /translation="MAEASKDNPSFMQGAAPRTVLFAKSSISGKLSYNLPVKIDGHFT
                     GEIKSTELLVIGPNALVEAHVSARQVHLEGKLIGSVQAMGCLEILAG"
     gene            384..1709
                     /locus_tag="VE170_07985"
     CDS             384..1709
                     /locus_tag="VE170_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYJ15413.1"
                     /translation="MDLFDTVPAKANLAAKPLAEAMRPTSLDEFVGQEHLLGAGKLLR
                     GLSGAGKLRSLILWGPPGSGKTTLAQILASSAGAHCVHFSAVTSGVKDLKKIIQDAEQ
                     LQRLGKPTVLFVDEIHHFNKSQQDNFLPHVERGTLILIGATTENPSFEVIAPLLSRCQ
                     VLVLKPLSPENMATIVDRALSDGPRGLGKMKLTLSAEGRDFIMQQSQGDCRVALNTLE
                     SAAALAQAGRQSEISLIHLQEALQKKPLQYDKAGDEHYNVISAFIKSMRGSDPDAALY
                     WMMRMLEAGEEPLFIARRMVIFASEDIGNADPQALQVAIAAKDAVHFVGLPEGKIPLA
                     HAVTYLASAPKSNASYKAMLAAAQDVETHGALAVPLHLRNAPTGLMKNLGYGSGYQYA
                     HNYQDQIVDQQHLPDELAGKSYYIPGDSGYEKQIKERLKSWDEKKKHKR"
     gene            complement(2027..2866)
                     /locus_tag="VE170_07990"
     CDS             complement(2027..2866)
                     /locus_tag="VE170_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ15414.1"
                     /translation="MAITTYNQVSVPSFMYGTAWKKEATARLVQLAVESGFTAIDTAN
                     QLIHYQEALVGEALQSLAAKGIQREALFLQTKFTSVDGQDHRTPYDPAADLTTQVEQS
                     FASSLQHLHTDYLDSYVLHAPFSRRGLGAADWQVWAAMEAFYEAGKTKTIGVSNISAA
                     QLILLCQQARVKPMMVQNRCFAVTAWDQQVREICRQQGIIYQGFSLLTANRDVMAAPE
                     IHAIAAREGVGPAQVIFRFALQVGMLPLTGTTDPQHMSEDLAAARITLLAEEVERIET
                     LAL"
     gene            complement(2987..5527)
                     /gene="hrpB"
                     /locus_tag="VE170_07995"
     CDS             complement(2987..5527)
                     /gene="hrpB"
                     /locus_tag="VE170_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HYJ15415.1"
                     /translation="MRQVDLPIFEIEQRLCDALSTGSRLVLTAPTGSGKSTQVPQMLL
                     DGGLLGAGQVTVLQPRRLPTRMLASWVAQARGVKLGGEVGYRMRLDNVASAATRICYV
                     TEGILLRQMLADPQLNGVSALIFDEFHERHLYGDISLARALQIQETTRPDLIIIVMSA
                     TLDVGAVGKFLHPCTLLASQGRTFPVDIEYLKQPTGDRPVWEVAVREAQRLVREYPEG
                     DMLIFMPGGYEIARTVQAARDQLGQQFVVFPLHGELAPSDQDAAVARYEKRKIVVATN
                     VAETSLTIDGVRLVIDSGLARIPRFDPYRGINTLLIEKISRAAADQRTGRAGRTAPGH
                     CLRLWTAHEQEARPFQELPEVKRLDLSEVILTLKASGVVDVKNFRWLEAPETRALTRA
                     ETLLADLGAIDKRSAAITELGRRMLAFPLHPRYARMLLAAQQYGCVRPVAAIAALTQG
                     RDLLLRPLNKLADGRGELFDDESASDLFVLLRAWRYAELNGFHPERCRRMGIHAQAAR
                     QVGPLFQQFLRIAGDEGLDISEKPDDNAAVQRCILLGFSDQLARRLDDKSLRCDLLHG
                     RRGLLARESVVRDAELFVVSEVREVESSVGRDRNLNVMLDLATAVNEEWLRELFPRDF
                     EEARTVTYDPVLRRVVARVERTFRGLVIAERLADNPPVDEAAAILAREVSAGRCVLEK
                     WNDTVEQWILRVNRLREWMAELALPAFAAEDRVALVEHICHGAFTYAQIKDRDVLPVV
                     KSWLSQQQQSWIDEYAPERIPLPRGRHAKVVYSADRPPTLAARIQDLYDIKDGIWIAN
                     RRVPVSIQVLAPSNRPVQVTENLAIFWRETYPQLKQQLQRRYPKHEWR"
     gene            5728..6591
                     /locus_tag="VE170_08000"
     CDS             5728..6591
                     /locus_tag="VE170_08000"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HYJ15416.1"
                     /translation="MHKRIVATLLVLIGLVLVAAVNINFFVARNKDYLLERLTRTLGH
                     TITADKIEIGYQPLAVRLTNLNIAGAATDATTPLVMAKESQINLRVLPLLLGQLQPGK
                     ITLDSAVVTIVRDLDGRYNYEPQGGNRQSARARSETIAPDRQLFAITALQVTNGTLRY
                     RDLNNGDDLIVAQIRLTLSDLAPDEAIDIDLEAAVMAAKSNFKFTSRIGPIAEIRNYR
                     DFPIEGALDATQLDIGKVNRALPQLRKATPRHLRFDGIYDIKSLKFKGTLNNLSLKGA
                     VSGTDASFRFE"
BASE COUNT         1516 a         2128 c         1888 g         1394 t
ORIGIN      
        1 cgcccgctaa aatttccaaa cagcccatgg cctgcaccga gccaataagt ttgccctcga
       61 ggtgcacctg acgcgccgac acatgggcct cgaccagggc gttggggccg atcaccaaaa
      121 gttccgtgga tttgatttcg ccggtgaaat gaccgtcgat cttgaccggc agattgtaac
      181 tcagttttcc cgagatcgac gacttggcga atagcaccgt gcgcggcgcc gcgccctgca
      241 tgaatgatgg attatccttt gaagcttctg ccatggctgt ttgtccctgc tggagcgtgc
      301 aacatagcac cgactgacgg cgagcggaag caaatgttgg cagctacaat ccaaccgcgc
      361 aattgtgcta gcattaaata gacatggacc tttttgacac ggtgccagcc aaagccaatc
      421 tcgcggcaaa accgctggcc gaagcgatgc ggccaacctc cctcgacgag tttgtcggcc
      481 aagaacattt actcggcgcc ggaaaactgc tgcgaggact gagcggcgcc gggaaactgc
      541 gctcactgat tctctggggc ccgccaggga gcggcaaaac cacgttggcg cagattctcg
      601 ccagctccgc cggggctcac tgcgtgcatt tctcagccgt gacttcggga gtcaaagatt
      661 taaaaaaaat cattcaggat gctgaacagc tgcagcgact gggtaaaccg acggtcttgt
      721 tcgtcgatga gatccatcac ttcaacaaat cccagcagga taactttctt ccccatgtcg
      781 aacggggcac gctgattctg atcggcgcga cgacggagaa cccgtccttc gaagtcatcg
      841 ctcccctgct ctcgcgctgc caggtgctcg tgctcaaacc gctatcgccg gaaaacatgg
      901 caacgatcgt cgaccgcgct ctcagcgatg gcccgcgcgg cctcggtaaa atgaagctga
      961 cgctctccgc tgagggccgc gattttatca tgcaacaatc ccagggcgac tgccgggtgg
     1021 cgctcaacac cctggaaagc gccgcggcgt tggctcaagc cggccggcaa agtgaaatca
     1081 gcctgatcca tctacaagag gcgctgcaaa agaaaccttt acaatacgat aaagccggcg
     1141 acgagcacta caacgtcatc tcggctttta tcaaaagcat gcgcggcagc gaccccgacg
     1201 cggcgcttta ttggatgatg cgcatgctgg aggctggcga agagccgttg ttcatcgccc
     1261 gacgcatggt gatcttcgcc tcggaagaca tcggcaatgc cgatccccaa gctctgcaag
     1321 ttgccatcgc cgccaaggac gcggttcact ttgtcggtct gccggaaggc aagattccct
     1381 tggcgcacgc cgtcacctac ctcgccagcg cgccaaagtc caacgcgtct tataaagcga
     1441 tgctggcggc ggcccaagac gtcgagaccc acggcgcgtt ggcggtaccg ctgcacctgc
     1501 gcaacgcccc caccggtctc atgaaaaact tggggtacgg cagcggttat cagtatgccc
     1561 acaactacca ggaccagatt gtcgaccaac agcatctgcc cgacgagctc gccggcaaga
     1621 gctactacat ccccggcgat tccggctacg agaaacaaat caaagaacgg ctgaaatcct
     1681 gggacgagaa aaagaagcac aagcgctgat caacctaacg gcacgaacgg cacggcagtc
     1741 ttcggcaaac aaaatctccg gccttcgtct acgtgtgtct cctcaaaaaa tacaggtaag
     1801 gttacggttg ccaaaatgaa cagggtatct gggtttcgtc atacccgcga aagcgggtat
     1861 ccaggctaat tgggcgcaga tcaaacctgg tttcccgcgt gcgcgggaat gacccgtcga
     1921 gtaggactgc ctatggagcg aatcccatgc attttcatat tctgtagacg agcgtaagac
     1981 catgatttac ttcgtggttc agaggagttt acatatcggc aatgcctcac agcgcgaggg
     2041 tttcgatccg ttcaacttcc tcggctaaga gtgtgatgcg tgccgcggcg agatcctctg
     2101 acatatgttg cgggtcggtg gtgccggtca gtggcaacat gccgacctgc agcgcgaaac
     2161 gaaaaatcac ctgcgccggc ccaacaccct cgcgcgcggc aatggcatgt atttccggcg
     2221 cagccatcac atcgcgattg gccgtaagca acgagaagcc ttgataaatg atcccctgct
     2281 gtcggcagat ctcacggacc tgctggtccc aggccgtcac cgcgaagcag cgattttgca
     2341 ccatcatcgg tttcacccga gcttgctgac agagcaggat caattgcgcc gcgctgatgt
     2401 tgctgacgcc gatggttttg gtttttcccg cttcataaaa tgcttccatc gccgcccaca
     2461 cttgccaatc ggccgcaccc agtccccggc gcgaaaacgg cgcgtgcagc acataggagt
     2521 ccaaatagtc ggtatgcaaa tgttgcagcg agctggcgaa ggactgctcg acctgagtgg
     2581 taagatccgc ggcgggatca taaggggttc gatggtcctg gccgtctact gaagtaaatt
     2641 tggtttgcaa aaataacgct tcgcgctgga tgcctttcgc cgccaacgac tgcaacgcct
     2701 cgccgaccag cgcttcctgg taatgtatca attgattggc cgtatctatg gccgtgaagc
     2761 cggactcaac cgctagttga accaagcgcg ccgtcgcctc ttttttccaa gccgttccgt
     2821 acatgaacga tggaaccgaa acctgattgt aggtagttat ggccatttcc cttcctctcg
     2881 ctagaccttg gcaatttcat cgttgtatcg caagggcgag taccgaggta ggtccgccgt
     2941 ggtttttacg ctcggtcaaa aatttcgagt ccggcacgga tgtcactcag cgccattcat
     3001 gttttggata gcggcgttgc aattgctgtt tgagttgcgg ataagtctcg cgccaaaaga
     3061 tcgcaagatt ttcggtgacc tgcaccgggc gattacttgg cgcgagcacc tgaatgctca
     3121 ccgggacgcg ccggttagcg atccagatgc cgtctttaat atcgtacaaa tcctgaatcc
     3181 gcgccgcgag ggtcggcggc cgatccgctg aatagaccac cttagcatgg cggccgcgcg
     3241 gcaacgggat gcgctccggc gcatactcgt cgatccagct ttgttgctgc tgcgacagcc
     3301 aggatttgac caccggcaac acgtcgcgat ccttgatctg cgcataggta aaggccccat
     3361 ggcaaatgtg ctcgacgagt gcgacgcgat cttcggctgc gaacgccggc aatgccaact
     3421 ccgccatcca ttcacgcagg cggtttacgc gcaagatcca ttgttcgacc gtatcgttcc
     3481 atttctccag cacgcagcgg ccggcgctga cctcgcgagc caagattgcc gccgcttcat
     3541 ccaccggcgg attatcggcg agcctttcgg cgatcaccaa accgcgaaag gttcgctcga
     3601 cccgcgccac tacccggcgc agcacggggt cataggtcac cgtccgcgct tcttcgaagt
     3661 cgcgcggaaa cagttcgcgc agccattcct cattaaccgc cgtggccagg tcgagcatga
     3721 cattcaaatt gcgatctcgg ccgacgctgc tctccacttc gcgcacttcg gatacgacaa
     3781 ataactccgc atcgcgcacc acgctttcac gggccaataa gccgcggcgg ccgtggagca
     3841 aatcgcagcg caacgatttg tcatctaggc gtcgcgccag ctggtcggaa aaacccaaga
     3901 gaatgcagcg ttgcactgcc gcgttatcgt cgggtttctc actaatatcc aagccttcgt
     3961 cgccggcgat acgcagaaac tgttggaaca gcggtcccac ctggcgcgcc gcctgcgcgt
     4021 ggatgcccat gcgccggcag cgctcggggt gaaaaccgtt gagctcggcg tagcgccagg
     4081 cgcgcaagag gacgaaaaga tccgatgcgc tctcatcgtc gaatagctcg ccccgcccgt
     4141 ccgctagctt attcagtggg cgcaacagca ggtcacgtcc ttgggtcagc gcagcgatcg
     4201 ccgccactgg ccgcacgcag ccgtattgct gtgccgccaa gagcatgcgc gcgtagcgtg
     4261 gatggagcgg gaacgccagc atgcgccgtc ccagctcggt gatcgcggca cttcttttat
     4321 cgatcgcccc cagatctgct aacaaagtct cggcgcgggt caacgcgcgc gtttcgggcg
     4381 cttccaacca gcgaaagttt ttcacgtcga caacgccgct cgccttgagt gtgagaatga
     4441 cctccgagag atccaaacgc ttgacttcgg ggagttcctg gaacgggcgc gcctcttgct
     4501 cgtgagccgt ccacaagcgc agacaatggc cgggcgcggt acggccggca cgaccggttc
     4561 tttgatccgc cgcggcccgg ctgatttttt cgatgagcaa tgtattgatg ccacgataag
     4621 gatcgaagcg cggaattcgc gccaagccgc tatcaatgac caggcgtaca ccatcgatgg
     4681 ttagcgaagt ctcggcgaca ttggtggcga cgacgatctt gcgcttctca taacgcgcca
     4741 ccgccgcatc ctgatcgctc ggcgccagct cgccatgcag cggaaagacg acaaactgct
     4801 gcccgagctg gtcgcgcgcg gcctgcaccg tgcgggcaat ttcgtatccg cctggcatga
     4861 atatcagcat gtcgccttcg ggatattcgc gcaccagtcg ttgcgcctcg cgcaccgcga
     4921 cttcccaaac cggccggtcg ccggtcggct gcttcaagta ttcaatatca accggaaagg
     4981 tgcgcccctg ggacgccaac agggtgcacg gatgcagaaa ctttcccacc gcgccgacat
     5041 ccaacgtcgc cgacatgacg atgatgatga gatcgggccg ggttgtttcc tggatttgca
     5101 gcgcccgcgc cagggaaatg tcgccgtaga gatggcgttc gtggaattcg tcgaagatta
     5161 gcgcgctgac cccgttcagc tggggatcgg ccaacatctg gcgcaataga atcccctcag
     5221 tcacgtagca gatccgcgtg gccgccgagg cgacattgtc caagcgcatc cgatagccca
     5281 cctcgccgcc aagtttgaca ccgcgcgctt gcgccaccca gctggccaac atgcgggtcg
     5341 gcaagcgccg cggctgcaaa accgtcactt ggccggcgcc aagcaaaccg ccgtcgagga
     5401 gcatctgcgg cacctgtgtc gacttgcccg agccggtggg ggcggtcagc accagccttg
     5461 atcccgtcga caaagcatcg cacagccgtt gttcgatctc gaagatcggt aaatctactt
     5521 gcctcataga tcgagaaaac tttaacacaa ataacccagg ttgcggacca cgccgctcgc
     5581 caacagcgaa attatccggc gccgtttgta tatgtcaaaa tattacggca aggaatactg
     5641 tcgagcgcga gaaccggcgc aatgttctta tcgcggctaa taccgtcaat gcagtgaccc
     5701 acaactgtca aggaaatccc atcgctcatg cataaacgaa tagttgccac tcttctcgtt
     5761 ttaatcggcc tggtcctggt cgccgccgtc aatatcaact ttttcgtcgc gcgcaacaaa
     5821 gactacttgc tcgagcgcct cacccgaact ctcggtcaca ctattacggc ggacaaaatc
     5881 gagatcggtt accagcccct cgccgttcgt ctaaccaatc ttaacatcgc cggcgctgcc
     5941 accgacgcca cgactccgct tgtcatggct aaagagagcc aaataaatct gcgcgttcta
     6001 cccctgctcc tgggccagct gcagccaggt aaaatcaccc ttgactcagc ggttgtcacg
     6061 attgttcgcg accttgacgg gcgctataac tacgagcctc aaggaggaaa tcgccagtcc
     6121 gcccgcgccc gaagcgaaac gatcgccccc gatcgccagt tatttgccat cacagccctg
     6181 caggttacca atggcaccct gcgttaccgc gatttgaaca acggcgatga tctcattgtc
     6241 gcccagattc gactgacgct gagcgacctt gcgccggacg aagccataga catcgacctc
     6301 gaggccgcgg tgatggccgc caaaagtaat tttaagttca ccagccgcat cggcccgatc
     6361 gcggaaattc gtaactaccg agactttccg atagagggcg cgctcgacgc aacgcaactc
     6421 gatataggca aagtaaaccg agccctgccc cagctgcgca aggcgacgcc gagacacctg
     6481 cgcttcgacg gcatctacga tatcaaaagc cttaagttta agggcacgtt gaataatctg
     6541 tcgcttaagg gtgcggtgag cggcaccgat gcttcgtttc gcttcgaata acgatcgccg
     6601 gccatgggaa aaacttagcc cataaaccgg aagccgagag ttttctcact ctgcgccttt
     6661 actgaagata agcggagttt cgcttgcggc tgcgcggcgc tccgtcccgc gtggataaca
     6721 atttctttta ttgacgctca acgtctaagc ttcttgacta atccttcctg gaccatcttt
     6781 tctaaaatcg agttgtcaaa aaactctgtg gcgtgcgttc gctcgatttg tcgcccggcg
     6841 ttgcgttttc gaaggtcgcc gcatccgcga cggcaccaat ggcgcgttct tgacgatacc
     6901 cacggcgcgc gacggtggta gcacca
//
LOCUS       DASQKS010000308         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5612066, whole genome shotgun sequence.
ACCESSION   DASQKS010000308 DASQKS010000000
VERSION     DASQKS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5612066"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1218
                     /locus_tag="VE170_08005"
     CDS             271..1218
                     /locus_tag="VE170_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15417.1"
                     /translation="MSWYKHLITLVALLVSQPFSSRAEVVHVGISTPGLYELPTEIAQ
                     RKGFYREENLDARKVVIRTNLQVAALLAGELDYSTVSGLIARASIQNLPVKGVMGWFD
                     RPLHILIAKPNFKRLTDFKGKKIGVSGLGSAPHIILREALAQAGMNPDRDVTTLAVGG
                     SGDRLAALIAGTVDATPLDVAYVEKAEKLGLVSVIYFGDVVHTRLGGFGVSMDKIRKN
                     PAQITRMIRASLKGVRFIRDHKPETLAIMRDYLHVSADAAVKVHDFAMRSLNPDGLIA
                     KNTMDTEIRLAKEQLKLTEEITEEKIMDWRFLKEVLAQK"
     gene            1247..1642
                     /locus_tag="VE170_08010"
     CDS             1247..1642
                     /locus_tag="VE170_08010"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15418.1"
                     /translation="MAKIKHIAIRTHDIEKTAAFYKEAFGLEQVGVGQTGIYLTDGYL
                     NIAILNMRGVVEGETMKLGIDHVGFQVDDVDATVEKIRALGGKPLNQRNEVSHGDPST
                     PQSYFEVKCVVIDDQVIDVSNAGWIGAAK"
     gene            1753..3033
                     /locus_tag="VE170_08015"
     CDS             1753..3033
                     /locus_tag="VE170_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15419.1"
                     /translation="MSSSDIRKRISGLYYGWRMIGVVSALRILGGGLHQYGFTVFFLP
                     VSQDLGLTRASTSLAFSLARAEGSLTAPIVGYLLDRYGPKPLMLTAALFAGVGYILFA
                     FVHSYTTFLIVYLGVISLAFNAGFVHAPTVVANSWFIKLRARAMTVVSAAVPIGGAII
                     TPLLAVAVQNFGWRWAALIAGGLFIAGGIPLSLAIRRSPESVGMAPDGGPLELPQGDN
                     NGTPSTNNAVNEPEVSARQAFRSPIFWNLILSMTARSAAFTTVTTHFIPMMVWKGMSQ
                     TEASVLLAGFAIINLPIHFLLGWIADFVNKPKLVTLCLLLGFVAVLPMLWSDSLWALW
                     FFTALYSVLDASIPVFWASVGDFFGRKSFGAIRGNMNLFYTWGAVLGPVLAGYVYDRT
                     QSYALVFWAMAGSLLLSSAMTSLLIRPWANLRRG"
     gene            complement(3380..4363)
                     /locus_tag="VE170_08020"
     CDS             complement(3380..4363)
                     /locus_tag="VE170_08020"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HYJ15420.1"
                     /translation="MSTAAVSGGVVNCLGAPWPEDLSAAFIDQLHSQGVTLVGCTGNL
                     TWDDTLESIENFEVVKTIVRKHPDAYILRSGVDLERPENRGKVGVLLGLQNPKALSDS
                     LGLLDAFFDLGLRCLSLAFSENSYYGCGHASEVDTGLTSLGRQAIKRMNRLGIVIDLS
                     HSGDRTALEALELSEQPVIFSHSTSRTLFNRPRSAPDPMIVAAAKKGGVICQDVRANT
                     SVAEYVDWVDYCVQLAGIDHIGVAAQDDFHRSYKDTKRIAPYLPSYAAELKKRDWRDD
                     RVVRRGGIGAKLLDQENLPAEFARRNYSDEAIGKILGGNLMRVMHAVLPKS"
     gene            4481..>5437
                     /locus_tag="VE170_08025"
     CDS             4481..>5437
                     /locus_tag="VE170_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15421.1"
                     /translation="MANPPATFTGEIGIHYPLHVLNRYTLPELIGLADRALKIMGPFG
                     FTRVWTNDNLEYRSVLASSAAILASLPVKLGTAVTVPYFRNPIDVAMAFATMSELAGG
                     KEISLGLGPGSRSILTRHVVRTKPLTIMAELAVGLRSLFAGDTLKRDDIPTLADYFHL
                     NSEHYALRFKTAAPIHLYYGPSLLKPAVLDLVARHFDGVILQTLYGVADMEASLTRLN
                     DARAQFGAVEPLRKTMLLNASVSRDSQAARQHAKRFVSHIVSGWPDDALEAKSIDPKA
                     IQSVRQAYAENRGVDAAAALTPDEAVDRLIIAGTPGECVGRLR"
BASE COUNT         1090 a         1687 c         1499 g         1161 t
ORIGIN      
        1 cccaaaccga cgggccaacg ctggtgcaac aacggcgtgg ccctgaagtt cattgccaaa
       61 accgccaaag cagcggcgta aatcgccggt cgatgttcac cacgaaggca cgaatgtgcc
      121 gaggatctcg gataataatt attccgaact tcgcgctctt cgtgccttcg tggtgcaagg
      181 gatactcctc ccaaggtggg cacgacgctt ccgccgcacg gtgtaatgtg ttaactgacg
      241 gggtgcaacc agaggatctg aaggcgcccc atgtcttggt ataaacatct catcacgtta
      301 gttgcgctct tagtcagtca gcccttctca agccgagccg aagtcgttca cgtcggcata
      361 tcgacgcctg gcttgtatga actgcccacc gagatcgctc aacgcaaagg gttctatcgg
      421 gaagaaaacc tcgatgcgcg caaggtggtg attcggacca accttcaggt cgcggccttg
      481 cttgccggcg agctcgatta ctccacggtg agcggcctga ttgcgcgcgc ttcgattcag
      541 aacctcccgg tcaaaggggt catgggttgg ttcgaccggc ctctgcatat cttgatcgcc
      601 aagccgaact tcaaacgcct caccgacttc aaagggaaaa aaatcggcgt cagcggtttg
      661 ggttcggcgc cgcatatcat tttgcgcgaa gccctcgccc aagccggcat gaatcccgat
      721 cgcgacgtca cgaccctcgc ggtcggcggc tcgggggacc ggttggcggc attgattgcc
      781 ggcacggtgg acgccacgcc ccttgatgtc gcctatgtcg agaaggccga aaagctcggt
      841 ctcgtttcag tcatctactt cggcgacgtc gtgcatacgc ggctcggcgg cttcggcgtt
      901 tccatggaca agattcgcaa gaatccggcg caaatcactc gcatgatccg cgcctcgctc
      961 aaaggcgtgc gcttcatccg cgaccataag ccggagaccc tggcgatcat gcgagactat
     1021 ttgcacgtca gcgccgacgc cgcggtaaaa gtccacgact tcgccatgcg ctccctcaac
     1081 cccgacggtc tgatcgccaa gaacacaatg gacacggaaa tccgtctagc caaggaacag
     1141 ctcaagctca ccgaagagat caccgaagaa aaaatcatgg actggcgctt tctcaaagaa
     1201 gtgctggcac aaaagtaaac ttcgccgccg gttggaggat cgacacatgg ccaaaatcaa
     1261 acatatcgcg atcagaaccc acgacatcga gaaaaccgca gccttctaca aagaagcctt
     1321 cggcctcgaa caggtcggcg ttggccagac cggcatctat cttaccgacg gctatctcaa
     1381 catcgcgatc ctgaacatgc gcggcgtggt cgagggcgaa accatgaagt tgggcatcga
     1441 tcatgtcggc tttcaagtgg acgacgtcga tgccaccgtg gaaaagattc gcgccctcgg
     1501 cggcaaaccg ctcaatcaac gcaacgaagt cagtcacggc gatccgtcca cgccgcaatc
     1561 ctatttcgag gtcaagtgcg tcgtgatcga tgaccaagtg atcgatgttt ccaacgccgg
     1621 ctggatcggc gccgctaagt aaaccgtatt tgccgcttct aaaatctcgg cattcccact
     1681 agccattcgc cactgccagc gccgatagaa tcgccgtatc actggcaccg aaggcagagc
     1741 attggcgcaa ccgtgagcag ctccgacatc cgcaaacgca tctccggcct ctactacggt
     1801 tggcgcatga tcggcgtggt ctcggcgctg cgcatactcg gcggtgggct gcatcaatat
     1861 ggattcacgg tatttttcct gccggtcagc caggacctcg gcctgacccg cgcgtcgacc
     1921 tcgctggcgt tttctctggc gcgcgccgaa ggctccctca ccgcgcccat cgtcggctac
     1981 ctgctcgatc gctatgggcc gaagccgctc atgctcaccg ccgctctatt cgccggtgtc
     2041 ggctatatac tttttgcctt tgtgcacagc tacacgacgt ttttgatcgt ctatctcggc
     2101 gtcatctcac tggcatttaa tgccggcttc gtccacgcgc ccacggtggt cgccaacagt
     2161 tggttcatca agctgcgtgc ccgtgccatg accgtggtca gcgctgccgt gcccatcggc
     2221 ggcgccatta tcacgcctct gcttgccgtc gcggtgcaaa atttcggttg gcgctgggcc
     2281 gcgctgattg ccggcggttt gttcatcgcc ggaggaattc ctttgtcgct ggcgattcgc
     2341 cgctcgccgg aaagcgtcgg catggctccc gacggcggac cgttggaact ccctcagggc
     2401 gacaataacg gcacgccgtc gaccaacaac gctgtcaacg aaccggaggt cagcgcgcgc
     2461 caagcgtttc gctcgccgat cttctggaat ctgattctat ccatgaccgc gcgctcggcg
     2521 gctttcacca ctgtcacgac ccatttcatt cccatgatgg tgtggaaagg catgagccag
     2581 accgaagcat cggtgctgtt ggccggcttc gcgatcatca acttgccgat tcacttcctg
     2641 ctcggctgga tcgccgactt cgtcaacaaa cccaagttgg tgacgttatg tttgctcttg
     2701 ggctttgtcg cggtcttgcc gatgctgtgg agcgactcgc tctgggcgct gtggtttttc
     2761 accgcgctct attcggtgct cgacgcttcg atcccggtgt tctgggcttc ggtgggcgat
     2821 ttcttcggcc ggaaatcctt cggcgccatc cgcggcaata tgaatctctt ctacacttgg
     2881 ggcgccgtgc tcggcccggt cctcgccggc tacgtctacg accgcacgca gagctacgct
     2941 ttggttttct gggcgatggc aggatcgttg ctgctctctt ccgccatgac gtcgctattg
     3001 atcagaccct gggcgaatct gcggcgagga tgatcagatt tctccgcaat cctagccatg
     3061 cgccaattct cttctcgaaa catggatagg gacggcacag atggctgctc cctactagct
     3121 attccccctt tggcaaaggg ggacgaaggg ggatttgctt gtgcggtggg gtggttttct
     3181 tcgtaatcgt cgggttcgcc gctaaaaaaa tcgaggcgct tattccgaaa atcttccctg
     3241 gcccctcttt ttcaaaaggg gtaagcaaga cttgccacgt ctagagcgtt taagccaaat
     3301 ctgcctgcgt accctaaccg ccaagtgtgg gcgcaattga taggagcaac ggccaaagtc
     3361 gttggctatc tttgaaccat caacttttcg gcaacaccgc gtgcatcacc cgcattaaat
     3421 tgccgccaag aatctttccg atcgcctcgt cggaataatt gcgccgcgcg aattccgccg
     3481 gcaaattttc ttgatcgagc agtttggcgc cgatgccgcc gcgccgcacg acgcggtcgt
     3541 cgcgccaatc gcgtttcttg agttccgccg cgtaggaagg cagatagggc gcgatgcgct
     3601 tggtgtcttt gtaagaacga tggaaatcgt cctgcgccgc cacgccgata tgatcgatgc
     3661 cggcgagttg tacacagtag tcgacccagt cgacatactc cgcgacgctg gtattggcgc
     3721 gcacgtcttg gcagatcacc ccgcccttct tggccgccgc gacaatcatc ggatcggggg
     3781 cgctgcgcgg ccggttgaat aacgtccggc tggtcgagtg gctaaagatc accggctgct
     3841 ccgagagttc aagcgcttcg agcgccgtgc ggtcgccgga atgggacaga tcgatgacga
     3901 tgccaaggcg attcatgcgc ttgatcgcct ggcggccgag cgacgtgaga ccggtatcga
     3961 cttcgctggc atggccgcag ccgtaatagc tattttcgct gaaggccaag gacagacagc
     4021 gcaagccgag atcgaagaac gcgtcgagca accccaaact gtcgctcaac gccttgggat
     4081 tttgcaggcc aagcaacacg ccgactttgc cgcgattttc aggacgctcg agatccaccc
     4141 cactgcgcag gatgtaggcg tccggatgct tgcggacaat agtcttaacg acttcgaagt
     4201 tctcgatgct ttccagcgta tcatcccaag taaggttgcc agtgcagccg accagcgtga
     4261 cgccttggct gtgcaattga tcgatgaacg ccgccgacag atcttccggc cacggcgcgc
     4321 cgagacagtt cacgacgcca cctgatactg ccgctgtact cataaacacc tcctcgatta
     4381 gatggaaaat acgccaaccg gtaatcgata tccgccccgg gtgcaagccg cggtgctaat
     4441 ggctttgact taagcgacgc gaccgtgcta cctcgattcc atggccaacc caccagcaac
     4501 tttcaccggc gagatcggca ttcattatcc gctccacgtg ctcaaccgct ataccctgcc
     4561 ggaactcatc ggcctggcgg atcgcgcgtt gaaaattatg gggccgtttg gcttcacccg
     4621 ggtgtggacc aatgacaatt tagaataccg cagcgtcctc gccagctccg cggctattct
     4681 ggccagctta ccggtgaagc tcggcaccgc cgtgacggtg ccttattttc gcaatccaat
     4741 tgacgtggcg atggctttcg ccaccatgtc cgaactcgcc ggcggcaaag aaatttccct
     4801 cggcttgggt ccgggttcgc gctcgatcct gacgcgccac gtcgttcgca ccaaaccgtt
     4861 gacgatcatg gcggaactcg cggtcggttt gcgcagcttg tttgccggcg acacgctcaa
     4921 acgcgacgac attccgaccc tggccgatta ttttcatcta aactcggaac attatgcgct
     4981 gcgcttcaaa actgccgcgc caatccacct ttactacggg ccaagcctgc tcaagcctgc
     5041 cgtgctcgat ttagtcgcgc gccacttcga cggggtaatt ttgcagaccc tttacggcgt
     5101 cgccgacatg gaagcttccc taacgcgact caatgacgcg cgcgcgcaat tcggagctgt
     5161 tgagccgctg cgcaaaacca tgctgctcaa cgcttcggta tcgcgcgaca gccaagccgc
     5221 ccggcaacac gccaaacgct ttgtttccca tatcgtatcg ggatggcccg atgacgcgct
     5281 ggaggcaaaa tccatcgatc ccaaggcgat tcagtcagtg cgccaggcct atgccgaaaa
     5341 tcgcggtgtc gacgccgccg ccgcgctcac acccgacgaa gcggtcgatc gtctgatcat
     5401 cgccggcacc ccgggcgagt gcgttggtcg attgcgt
//
LOCUS       DASQKS010000309         6139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5189995, whole genome shotgun sequence.
ACCESSION   DASQKS010000309 DASQKS010000000
VERSION     DASQKS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5189995"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VE170_08030"
     CDS             <1..578
                     /locus_tag="VE170_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ15422.1"
                     /translation="IGIVGRIFPIKNHALFLDAAARIFARERKARFIIVGDGVLRPKL
                     EKQARDLGIADRVLFTGWRRDLPRICADLDVLVVSSDNEGTPVSAIEAMAAGCPVVAT
                     RVGGLPDLIEDQSTGCLVPPREPETLANAVLGLFDDPQLARELGKHGRDSVRQRFTVQ
                     RLLGDMDDLYTQLLEEKTIASGAPEGLLVRR"
     gene            complement(819..1379)
                     /locus_tag="VE170_08035"
     CDS             complement(819..1379)
                     /locus_tag="VE170_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ15423.1"
                     /translation="MKKLLGLLVMSGAVTAALFLLTSLSGPGGGQSSQAITLKPGSQV
                     QDGSIVSIEYTLTGDDGKVIDTSAGKEPLTYIHGAGQIVKGLEQELNGLKVGDQKKVS
                     VKPEDGYGMPSEKAFQEIPRENVPAEALKAGATLMMKSPDGRAMPITIAQVKEKTVVV
                     DLNHPLAGKTLHFDVKVKDIKAAEAR"
     gene            1533..2393
                     /locus_tag="VE170_08040"
     CDS             1533..2393
                     /locus_tag="VE170_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ15424.1"
                     /translation="MSGEIWALLSAAAWAVDSILVRKGTAFSNASTAAFVSFLVSVLF
                     LVPYIFWQFPSEKIFHSANLYFVASGVIQPAMVRVLFYIGIIRLGVSRAGPLRGTAPL
                     FTMALAFFVLSERPGPFVYAGAILTVLGTWLVSYRRAGEANWRTVDILFPLGAALLAS
                     VSQNIRKLGLLTMDEPLIASTVSSTTSFLCLLVSLGVSGTLRSLKFRGGCLPYYVAAA
                     LFALVGQLFNFHALHAGEVSVVSPIINTTPLFIIAFTAVFLRGEEKITKLVVIGAIML
                     VAGIAVITGR"
     gene            complement(2535..2960)
                     /locus_tag="VE170_08045"
     CDS             complement(2535..2960)
                     /locus_tag="VE170_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HYJ15425.1"
                     /translation="MAEEKAFKKPLPEVTPVNQPFWDGAKAGKLMMQKCANCSTWVFP
                     PRPVCGDCASEKLDWVQVSGTGKVFCFTIIREVVGTALRGFGPDIPYVTAWVDLDDGP
                     RLCSNVIGCPIEQVKIDMPVRAVFEDSGDGITLPKFKPA"
     gene            complement(3220..4413)
                     /locus_tag="VE170_08050"
     CDS             complement(3220..4413)
                     /locus_tag="VE170_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYJ15426.1"
                     /translation="MSIRGKTAVVGIGETPTDRLGGKPGEPRKSTAEYLAWAARLAIE
                     DAGLTKKDFDGQGLAAIYTTNHSQPFWPEEVAAILGISPGVSLAGGNGGASSVSLLGH
                     AAAAISSGLCDLVLVIAAAAPFSEHGGHRGAHGDTRDFEMPFGVMGPNCKISFVMSRY
                     MHESGMTDDHFGKVAVTGRYHASLNPNAYLRKPITIEDYKKSRMISDPIRLFDCVMPA
                     NGGKAYIMTSAERAKTMRKPPVYLLGWGECNNASFGPRYRANPLITGITDAGKRAFAM
                     SGVAHKDIRCLNLYDDYIPVVMIQIEDLGFCGRNDKSFFEKTDFTFKGDLPIQTSGGM
                     INCGQPSTTGGMLHVIETVRQIRGEGGERQVPNIKFGISTGVGAVNYGKNFGCTAAAI
                     LGSEA"
     gene            4540..5523
                     /locus_tag="VE170_08055"
     CDS             4540..5523
                     /locus_tag="VE170_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15427.1"
                     /translation="MKFGKGFALALLGLFLAQYNTAAAAEKLIADYGGHAGFQSATWI
                     GKDLKIFEKHGLDVEVIMITGAARSVAALLGNSTHFATGSATGPLAAAVRGSDIKVIA
                     ASYNKFPYAFVVRPDIRSPNDLRGKRVNILNYGGSNDLALQLALREWGLKLADIQIVI
                     GGDAPTRLGSLMAGRMDATILSPPHLTIAVKAGYRVLADMGDMSSNFTQSSLYVKGSS
                     LRENRDRVKRFLRAYAEAIHTIKTDRERTMKVFAKRMRVEDPETVRSTYDYFAPRFSF
                     PPRVNMSGVRDTINFYAEQNPDFKNRKAEEFVDHSLLDELEKEGFFKKLGS"
     gene            complement(5575..>6139)
                     /locus_tag="VE170_08060"
     CDS             complement(5575..>6139)
                     /locus_tag="VE170_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15428.1"
                     /translation="ISSFGGTPHSEAIAILKKYNLNPEKDVTFLQVGGSSARYMSLES
                     GSIQAAMIVPPFNHLAKKRGFNQLLGFNDIMSMPVGGLAVHTQKMKEKPDEIVKMIRA
                     LIKSLEFIRTRKADVLAIIDKQWGIKEADVREEMYREMIGLFSRTGIGADESMRNVIR
                     LVRETRKNAPEVAIADVADWSFARKAQ"
BASE COUNT         1176 a         1686 c         1804 g         1473 t
ORIGIN      
        1 tgatcggaat cgtcgggcgg atttttccca taaaaaatca cgccctcttt cttgatgccg
       61 cggcgcgtat tttcgctcgc gaacggaagg ctcgtttcat tatcgtcggc gacggtgttt
      121 tgcgcccgaa gttggaaaaa caagcacgcg atttaggtat cgccgaccga gttctcttca
      181 ctggttggcg acgtgacctt ccacggattt gcgctgatct agatgtgctg gtggtttctt
      241 cggacaacga aggcacgccg gtctcggcga tcgaggcgat ggccgcaggg tgtccggtgg
      301 tcgctactcg ggtcggcggg ctaccggatc tgatcgaaga tcaatccacc ggatgtttag
      361 ttccgcccag ggaacctgaa acgctcgcta acgctgtcct tggtttattc gatgatccgc
      421 aattggcgcg cgaactgggt aaacatggcc gcgactcggt gcggcagcgt ttcaccgtcc
      481 agcgcttgct cggcgacatg gatgatctct atacccaact attggaggag aagacgatag
      541 cgtctggcgc gcctgaaggt ttgctggttc ggagatagta cggcttcgtg agcagtcatt
      601 agacttctcg gccgggtgtt gctgtccggc tctttcggaa atgtactata gcggccgtcg
      661 cggactgccg cgcaaaaatt gaccgtggca aatcatagtc cactttgatc ggatctatta
      721 cgccgctggg cgggcatgtc ttcttgtaga catgcccgcc ctttcttttt tatgtataga
      781 gtccgtccga aatagcttta ctgaatttgc gtcgtggcct atctggcttc cgcggctttg
      841 atgtccttca ctttgacatc gaagtgcagc gtcttgccgg cgaggggatg attcaagtcg
      901 accactacgg tcttttcctt cacttgcgct atggttatcg gcatggcgcg gccgtcgggc
      961 gacttcatca tcagcgtcgc gcctgctttt agcgcctcgg caggaacgtt ttcccggggg
     1021 atttcctgga aggctttttc gctcggcatg ccgtagccgt cctccggttt aacgctgact
     1081 ttcttctgat cgccgacctt gaggccgttg agttcctgct ccaagccttt gacgatttgt
     1141 ccggcgccgt ggatgtaggt gagcggttct ttgccggcgc tggtatcgat aactttgccg
     1201 tcgtcaccgg tgagagtgta ttcgatggag acgatggagc cgtcttgcac ctgggatccc
     1261 ggcttgagtg tgatcgcctg actggactgc ccgccgccgg gaccggaaag gctggtcaac
     1321 agaaacaacg ccgcggtaac cgccccgctc atcaccaaca agcctaatag ttttttcatg
     1381 aattcccagg cgtagagata acggattgaa ttcgcgccaa catatattgt gaaagcattg
     1441 aatacaacta aaacgccgcc gtcggctggt atcttggcga cggcggctgt gctaagtctc
     1501 cgaaatcggc aagaaagttt ggctagcaag gaatgagcgg cgagatctgg gcactgttat
     1561 cggcggcggc gtgggcggtg gattcgatcc tcgtgcgcaa gggcacggcg ttttccaacg
     1621 cgtcgaccgc ggcgtttgtc agctttctcg tttcggtgtt gtttcttgtg ccgtacatct
     1681 tctggcagtt tcccagcgaa aagatttttc attcggcgaa tttgtacttc gtcgccagcg
     1741 gcgtgatcca accggcgatg gtgcgggtgc tgttctacat cggcatcatt cgccttggcg
     1801 tgtcgcgtgc cgggccgctg cgcggcacgg cgccgctgtt taccatggca ctggcgtttt
     1861 tcgtcttgag cgaacggcct gggccgttcg tctatgccgg cgcgattctt actgtgctgg
     1921 gaacctggct ggtttcttac cgccgcgcgg gcgaagccaa ctggcgcaca gtcgatatcc
     1981 tgtttccgct cggcgcggcg ctgctcgcct cggtgtcgca gaatatccgc aagttgggct
     2041 tgctgactat ggacgagccg ttgatcgctt cgacggtcag cagcacgacg tcgtttttat
     2101 gcctgcttgt ctcgctcggg gtttcaggga cactccgatc actcaaattc cgcggcggtt
     2161 gtttgcccta ctacgtcgcc gccgcgctat tcgcactggt cggtcagctg tttaactttc
     2221 acgccttgca cgccggcgag gtctcggtgg tcagtccgat catcaacacc acgccgttgt
     2281 tcatcatcgc ttttacggcg gtatttttgc gcggcgagga gaagattacc aagctggtgg
     2341 tgatcggcgc gatcatgtta gtggcgggga tcgcggtgat taccggacgt taatccctgc
     2401 gagtgagtca gcaagttcac cgcagaggcg cggaggatgc cgaggttcaa aaattgtttg
     2461 cgacaaccct ccgcttctgc gtaaatctcg gcgtctctgc ggtgaacgag aactttgccg
     2521 atggcgtggc gaatctacgc cggtttaaac ttcggcaggg taatgccgtc gcccgaatct
     2581 tcgaaaacgg cccgcaccgg catgtcgatc ttcacttgct cgatggggca gccgatgacg
     2641 ttgctgcaca ggcgcgggcc gtcgtcgaga tcgacccagg cggtgacata agggatatct
     2701 gggccgaaac cgcgcagtgc ggtgccgacg acttcgcgaa tgatcgtgaa gcaaaacact
     2761 ttgcctgtgc cgctgacctg cacccaatcg agcttttcgc tggcgcaatc gccgcatacg
     2821 ggacgcggcg ggaagaccca ggtcgagcag ttggcgcatt tctgcatcat cagtttgccg
     2881 gctttggcgc cgtcccagaa aggttggttg accggggtta cttcagggag aggtttttta
     2941 aatgcttttt cttctgccat ttttcttacc tttcacaagg tttgtcgaat tttcacttta
     3001 gaggcgaagg gtgacaaatt acgacgttca ataagtttaa accgttcaaa ccgttcaagc
     3061 ccccaccact cttcgaagct gttccggtgg ggctttcctc ccccgcgtcg cggaggagga
     3121 taaaagaggg ggattgaacg gagatcgcaa agcgatcgat tgaacggagc gtagcgattg
     3181 aacgttttga acaggcgcca gctccagact tcttacccct tacgcttcgc tgcccaagat
     3241 cgctgcggcg gtgcagccga aattcttgcc gtagttgacg gcgccgacgc cggtgctgat
     3301 accgaacttg atattcggta cctggcgctc gccgccttca ccgcggattt gccgcaccgt
     3361 ttcgatgaca tgcagcatgc cgccggtggt tgatggctgg ccgcagttga tcatgccgcc
     3421 gctggtttgg atcggcagat cgcctttgaa ggtgaaatcg gttttctcga aaaaggattt
     3481 gtcgttacgg ccgcaaaagc cgagatcctc gatttgaatc atgaccaccg gaatatagtc
     3541 gtcgtagaga ttcaaacagc gtatatcttt gtgcgcgacg cccgacatgg cgaaggcgcg
     3601 cttgccagca tcggtaatgc cggtaattaa cggattggcg cgatagcgcg gcccgaagct
     3661 ggcgttgttg cattcgcccc agccgagtaa atagaccggt ggcttgcgca tggtttttgc
     3721 ccgctccgcc gatgtcatga tgtaggcttt gccgccgttg gccggcatga cgcagtcgaa
     3781 caggcgaatc gggtctgaga tcatacgcga ttttttataa tcttcgatgg tgattggctt
     3841 gcgcaaatag gcgttgggat tgagcgaggc gtgataacgg ccggtgacgg cgactttgcc
     3901 gaaatggtca tcggtcatac ccgactcgtg catgtagcgg ctcatgacga aagaaatctt
     3961 gcaattgggt cccatgacgc cgaaaggcat ttcaaaatcg cgggtgtcgc cgtgggcgcc
     4021 gcgatgaccg ccatgctcgc taaacggcgc ggctgcggcg atgaccaaca ctaaatcaca
     4081 aagcccggag gagatcgccg cagctgcgtg gcccaacagg gacactgagc tggcgccgcc
     4141 gttgccgccg gcgagggata ctcccggcga gatgccgagt atcgccgcca cttcttcggg
     4201 ccagaagggc tgcgaatgat tggtggtgta gatcgccgcc agaccctggc cgtcgaaatc
     4261 ttttttagtt aagccggcgt cttcgatggc taggcgggcc gcccaggcga gatactcggc
     4321 tgtcgatttg cgcggctcac cgggcttgcc accgagccgg tccgtcgggg tttcgccgat
     4381 gccaaccact gcggtttttc ctctgatgct catataggaa ttcctttctt ggtctttgga
     4441 gagatatttc agcgccgtgg ggctgtcaag caaagcgcgc aactatcttg accgtatgga
     4501 agcaaacccc tattattccg ccagccgaaa ggagcggaaa tgaaatttgg caaagggttt
     4561 gcgcttgctc tgttgggtct ttttctcgct cagtacaaca cagcagcagc ggctgaaaaa
     4621 cttatcgccg attacggcgg tcatgctggt tttcaaagcg cgacttggat tggcaaggat
     4681 ttaaagattt tcgagaagca cgggctcgac gtcgaggtga tcatgatcac cggcgcagcc
     4741 cgctccgtcg ccgcgctgct gggtaacagc acgcactttg ccaccggatc ggcgacgggg
     4801 ccgctcgcgg ccgcggtcag aggctctgac attaaagtca tcgccgcgtc ttacaacaaa
     4861 ttcccctacg ccttcgttgt ccggcccgac attcgctcgc ccaacgattt gcgcggcaag
     4921 cgggtcaata ttctcaacta cggcggctcc aacgatctcg ccctgcaact cgcgctcaga
     4981 gaatggggtt tgaaactcgc cgatattcaa atcgttatcg gcggcgacgc gccgacgcgc
     5041 ttgggttcgc tgatggcggg gcgcatggat gcgacgattc tctcgccgcc gcatctgacg
     5101 atcgcggtca aagccggcta tcgcgtgtta gccgacatgg gtgatatgag cagtaacttc
     5161 acccaatcgt ctttatacgt gaaagggagc tcacttaggg agaatcgcga ccgcgtaaaa
     5221 cgcttcctgc gcgcctacgc cgaagcgatc cacacgatca agacagatcg cgaaagaacc
     5281 atgaaggttt ttgccaaacg catgcgggtc gaagatcctg agacggtacg ctcgacctac
     5341 gattacttcg cgccgcgctt ttcgtttccg ccgcgggtta acatgagcgg cgtgcgcgat
     5401 acgatcaatt tctacgccga gcaaaacccc gacttcaaaa atcgcaaggc ggaagagttt
     5461 gtcgatcact cgttactgga cgaactggaa aaagaggggt tctttaagaa gctgggaagc
     5521 tgaggatcga aacttggggt tggttttcca agaggcgtcc ggataccatt cacttcactg
     5581 cgctttgcgc gcgaagctcc aatccgccac gtcggcgatg gcgacttccg gcgcgttttt
     5641 gcgggtctcg cgcaccagcc ggatgacgtt gcgcatgctt tcgtcggcgc cgatgccggt
     5701 gcggctgaac aggccgatca tttcgcggta catctcttcc cggacatcgg cttctttgat
     5761 gccccattgc ttgtcgatga ttgccaggac atcggctttg cgcgtgcgaa tgaattccaa
     5821 gcttttgatc aaggcgcgaa tcatcttgac gatctcgtcg ggcttctctt tcatcttttg
     5881 cgtgtgcacc gccaaaccgc cgaccggcat gctcatgatg tcgttgaagc cgagcaattg
     5941 attgaagccg cgcttcttgg ccagatgatt gaacggcggc acgatcatgg cggcttggat
     6001 cgagccgctc tccagcgaca tgtagcgcgc cgagctgccg ccgacctgca gaaaggtcac
     6061 gtctttttcc ggattcaggt tatatttttt caagatcgcg atggcttcgc tgtgcggcgt
     6121 gccaccgaag ctgctgatg
//
LOCUS       DASQKS010000310         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_584262, whole genome shotgun sequence.
ACCESSION   DASQKS010000310 DASQKS010000000
VERSION     DASQKS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584262"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VE170_08065"
     CDS             complement(<1..702)
                     /locus_tag="VE170_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ15429.1"
                     /translation="MITTSIRVLSIIPPMTQLNTPYPSTAYLTGFLRSRGIDAVQEDL
                     ALALVLKLFSADGLLAIRECSEAIPKRKRSAIVNGFHRHFDRYLSTITPTIAFLQGRD
                     PTLAHRICGREFLPEGPRFKPIDFYLQDGNDDPLAWAFGALGIEDRAKHLATLYLDDL
                     ADALRDAVDPRFEFVRYAESLAQSQPTFDPLAEALTAPLNLVDRTLRDLTLAALARHS
                     PTVVLLSVPFPGSIYA"
     gene            complement(745..1710)
                     /locus_tag="VE170_08070"
     CDS             complement(745..1710)
                     /locus_tag="VE170_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15430.1"
                     /translation="MRRSILPSAVSFIVLFGIVSVNHAAEKIRMSVSGSYNMIFLAAG
                     VAQHKGFFKDEGLDADIVVMTAPASLAALSNGDIDYTLLTGSVIRAAIRGLPVRIVAG
                     LMSSSAHVFLARPEIRTIKELSGKRIGLAGFGDATHVLARIILAKHGVDPDKDVQFIG
                     LGSDSGRFTALQQNLADMVVTSPPWDFEGKKLGYNILARAYEHVNYPLAGLGVNQKSL
                     QTNRAQVKRVVKSLVRANRFMRDNREESVKILIGWGKGKSEHAYAAYDSTVKIISPDG
                     SIPDDGLKILIDQAKRDVKVSRDVPVSEITDFSILREVQKELGLR"
     gene            1909..2283
                     /locus_tag="VE170_08075"
     CDS             1909..2283
                     /locus_tag="VE170_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15431.1"
                     /translation="MRVLKINDLKETPMDTATPIAGWTGGAVSRTRQAVIGDGQSDNF
                     RCNVVNFRIGATTGWHVHDCDQILVVTAGKGFAATESEQREIIAGDIVHIKAGERHWH
                     GASAESPLSHITVTTTGSTSKH"
     gene            2472..2810
                     /locus_tag="VE170_08080"
     CDS             2472..2810
                     /locus_tag="VE170_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15432.1"
                     /translation="MADPVLAEMVHATGATESRHYAAVKPTIQAYRASWVRLAPFRDG
                     PLNPQRETVSDPTAMTARIKARATELGADMVGICRLQPHMIDLGSEVANEFEARRPNW
                     LVDIPTLWAR"
     gene            complement(2995..>3899)
                     /locus_tag="VE170_08085"
     CDS             complement(2995..>3899)
                     /locus_tag="VE170_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15433.1"
                     /translation="IRTVVSVLQKHIPGNPTIVVKYMPGGGGRMAANQLYKSTAPDGL
                     TMGAMLSSYIPAAVVGDPGALYDVQKMIFLGTPYSGHPHIFISRRGAGADTLEKLRSI
                     PNLRLGAQSVGHTIYYTGRMFAYLIGLKEPKSVIGYSGPELDIAFQSGEVDARSNHPD
                     NPIRQGLLEKGIVNVHAMIEVPRGHKHPHPRYAQLPSLESFAKSETERKLVALHRTFQ
                     LSGAPFVLPPGTPNDQVTILQQAMRRTFNDSEFLKEYEKVGDDSPPLMPETLTQLIRD
                     LPRNDEVVDLFKKLFGADPLPPRP"
BASE COUNT          849 a         1046 c         1178 g          826 t
ORIGIN      
        1 ggcgtatatc gatccgggaa acggcaccga aagcaaaacc acggtcggtg agtggcgcgc
       61 caacgcggcc agtgtcagat cgcgcaacgt ccgatcgacg agattcagcg gcgcggtcag
      121 cgcttcggcg aggggatcga aagtcggctg gctttgcgcc agggattcgg catagcggac
      181 aaactcaaac cgcggatcga ccgcgtcgcg cagcgcatcg gcgagatcgt cgagatataa
      241 cgttgccaag tgtttggcac ggtcttcgat tcccaatgcg ccgaacgccc aagccagcgg
      301 gtcgtcgttg ccgtcttgga gataaaagtc gatgggttta aagcgcggcc cttcgggaag
      361 aaactcccgg ccgcagatgc ggtgcgccaa agtcgggtcg cgtccctgca agaaagcgat
      421 cgtcggcgtg atcgtcgata ggtagcggtc gaaatggcgg tgaaatccat tgacgatggc
      481 cgagcgcttg cgcttcggaa tcgcctcgct gcattcacgg atcgctaaca acccgtcggc
      541 ggaaaaaagt tttaatacca aagccagcgc gagatcttct tgcaccgcat cgataccgcg
      601 cgagcgcaaa aagccagtga gatacgccgt tgacggatac ggcgtattga gctgggtcat
      661 cggtggaatg atcgacagga cgcggatgga ggtggtgatc atgtcagggt ttgcgcccgg
      721 ggcttggcct atccaaccct atcatcaccg caaccccagc tctttttgaa cttctctcaa
      781 aatactaaag tcggtgatct cactcaccgg cacgtcgcgg ctgactttca catcgcgctt
      841 cgcctgatcg attaagatct tcagtccatc atccggtatg ctgccatcgg gactgataat
      901 tttcactgtg gaatcgtagg cggcataggc atgctcggat ttgcctttgc cccagccgat
      961 caagattttc accgactcct cgcggttgtc gcgcatgaaa cggttggccc ggaccaggga
     1021 tttcacaacc cgtttcactt gggcgcggtt ggtttgcaaa gatttttgat ttacgccgag
     1081 cccggctagc ggatagttca catgctcgta ggcgcgggca agaatgttgt agccgagctt
     1141 ttttccctcg aaatcccacg gcggcgaggt gacgaccatg tcggccaagt tctgttgcaa
     1201 ggcggtgaac cggccggagt cggagccgag cccgatgaat tggacgtctt tatccggatc
     1261 gacgccatgt ttggcgagaa ttattcgcgc gaggacatga gtggcatctc cgaagccggc
     1321 gagtccgatt cgtttaccgg agagctcttt gattgtccgt atttctgggc gggccaggaa
     1381 aacatgcgcg gaactactca ttaatccggc gacgatacgg acaggcagtc cacgaatggc
     1441 ggcgcggatc accgagccgg tgagcagcgt gtagtcgatg tcgccattcg ataacgcggc
     1501 gagggatgcc ggcgcagtca tgacgacgat gtcagcgtcc aagccttcgt ctttgaagaa
     1561 gcctttgtgt tgtgcaacgc cggcggcgag aaagatcata ttgtaactgc ccgataccga
     1621 catgcggatt ttctcggcgg cgtggtttac agacacgatg ccgaataaga cgataaaaga
     1681 aacggcactc ggaagaatcg atcgacgcat gatttgcctc atatttgaaa ttgctataac
     1741 tgccggtaat gcaatggtca accggcaacc tgcctcgaac acgatcagat acacgggaac
     1801 gcccttgacc cctttggcgc cagcggtgta gttgtggcga tgcgcaagag gcaatacgcc
     1861 catagcagag aattcggaat catcgacggc taaccgagga ggtcgaaaat gcgtgtactc
     1921 aaaattaacg atctgaagga gaccccgatg gacacggcga cgccgattgc cggttggacc
     1981 ggcggggcgg tgagccggac gcggcaggcg gtgatcggcg acgggcagtc ggataatttt
     2041 cgctgcaacg tggtcaactt ccgcatcggc gcgaccaccg gctggcatgt ccatgactgc
     2101 gatcagatac tcgtcgtgac ggcgggcaag ggttttgcgg caacggaatc cgaacagcga
     2161 gaaattatcg ccggcgacat cgtgcatatc aaagccggcg agcgccactg gcatggcgcg
     2221 tcggcggagt caccgctgtc gcatatcacc gtgacgacga cgggcagcac gtcgaagcat
     2281 taaaaaccgg gcgttcaaag gttcaaaagt tcaagggttc aaggtcagat taggaaccgg
     2341 attttctgac gttgaacctt aaaccgttga acggttgaac cgcgagcgcg ttcggagcgc
     2401 agcatcgacc taaaggttaa gcggtgaagt gatgaaacca tgcgtggcat aaccctagac
     2461 gcgagcaagg catggccgat ccggtgttgg ccgagatggt tcatgctact ggagcgacgg
     2521 agtcgcgcca ctacgcggcg gtaaagccga cgattcaagc ctatcgcgct agttgggtga
     2581 gactggcgcc gttccgcgat ggtccattga atccgcagcg ggagacggtg agcgatccga
     2641 cggcgatgac ggcgcgcatc aaggcgaggg cgaccgagct tggtgctgac atggtcggca
     2701 tctgccgctt gcagccgcac atgatcgacc tcggttcgga agtggcgaat gaatttgaag
     2761 ctcgacgacc taactggctg gttgacattc cgaccttgtg ggcgcgctag tccagttagt
     2821 caataacgtc ccgtgaattt cgcccaacgt gcccacgcag atcgtgaaat tctttcccat
     2881 gggttgctac gcctcctttg gttcgaagcc atcgtaactt ccttgcgagt atctgcgcgc
     2941 ctggaccatc atgcggagat acttaaagcg cgcttgaccg cttcgctaga tgccttacgg
     3001 ccgaggcggt agggggtcgg ctccgaagag ttttttgaac aggtcgacga cttcatcatt
     3061 gcgcggcaaa tcccggatga gctgggttag cgtttccggc atcagcggcg gcgagtcgtc
     3121 gccgactttt tcgtattctt tgagaaactc ggaatcattg aacgtgcggc gcatggcttg
     3181 ctgaagaatc gtcacctgat cattcggcgt gcctggcggc agcacgaacg gagccccgga
     3241 tagctgaaag gttcgatgca aggccacaag tttccgttcc gtctccgact ttgcgaagct
     3301 ctcgagactc ggcagctgtg cgtatcgtgg atgcggatgt ttgtgtcccc tgggaacttc
     3361 gatcatcgcg tgcacattga cgattccttt ttccagcagc ccttgccgaa tcgggttgtc
     3421 ggggtgatta gacctggcat ccacctcgcc gctttgaaag gctatatcga gctccggacc
     3481 gctatagccg atgacggact ttggctcctt gaggccgatg agataggcga acattcggcc
     3541 ggtatagtag atcgtgtgtc caaccgactg ggcacccaag cgcaggttgg gtatggatcg
     3601 caatttttcc agtgtgtccg ctcccgcgcc tcgccggctg ataaaaatat gtgggtggcc
     3661 gctatacggc gttccaagaa agatcatttt ctggacgtcg tagagggcgc cgggatcccc
     3721 aaccaccgct gcgggtatgt aagaggaaag catggcgccc atcgtcaaac catccggtgc
     3781 cgtcgatttg tagagctggt tggccgccat ccgcccgcct ccaccaggca tgtacttgac
     3841 aacgatggtc ggatttccag gaatatgctt ttgcagtacc gaaacgacgg ttctgatgc
//
LOCUS       DASQKS010000311         4511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1831983, whole genome shotgun sequence.
ACCESSION   DASQKS010000311 DASQKS010000000
VERSION     DASQKS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831983"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VE170_08090"
     CDS             <1..1220
                     /locus_tag="VE170_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HYJ15434.1"
                     /translation="EEARLISLGIMSGLRFHLPLGLFLDIGGGSVELAVANTTNTYCV
                     FSLPLGAVRLTEKLVAHDPPRNKEIKELREYVQARLKPVVRRIHKEKYTMAFGSGGTI
                     TALAETDTRVTGDPNRAGSLIVLRRQRLRALLEMLMNEPADERAKMISGDPKRADIII
                     AGGFVLHEIMTEIGLEYIFVSRRGLRDGLMVDLLQKRHSESGPWHPDADRAESLEQVC
                     QKYLYDAAHAHHVSQLALNLFYQLHDLHGLPENYASILHAAAMLHDIGQFLAGPKHHK
                     HSYYLIKSSGMNSFNHLDLDLVANVARYHRKAHPSPKHLGFSQLSPSNQDIVRKLSAL
                     VRVADAFDFRHNQQVDNLSCTFKKTRTLTLSAASAANLKEEIRWAGQKGKLIQEVFNV
                     DLIIETNKPSRAR"
     gene            1243..1683
                     /locus_tag="VE170_08095"
     CDS             1243..1683
                     /locus_tag="VE170_08095"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HYJ15435.1"
                     /translation="MRREIHNRNILFLCEDNAQLSQMAEATAKHLSPPKTRIFSAGIK
                     PGRIPPSVIKAMNEIGIPIADQKSKSVAEVPIDEIDLVVSFGDAHKQCAYLPRRTKIE
                     NWPVADELQSKKAGAEELDAVRGARDDIEKRVFALFMDYWRNVA"
     gene            1704..2228
                     /gene="sixA"
                     /locus_tag="VE170_08100"
     CDS             1704..2228
                     /gene="sixA"
                     /locus_tag="VE170_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HYJ15436.1"
                     /translation="MNLYLMRHGIAVAADDLGVIQDNERPLTSKGSKRIRRAARGIRR
                     LGIEFDGILTSPLLRARQTADLVATALDLQSRLEEITELAPECSVEQLIICLTRYQDR
                     DHLLLVGHQPLLGQTLDGLIEPTPGTKIHFDLGKGSLCRVEVDRLPVDKPGILRWLMT
                     AKQLRLLASRSTKN"
     gene            2236..3141
                     /locus_tag="VE170_08105"
     CDS             2236..3141
                     /locus_tag="VE170_08105"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HYJ15437.1"
                     /translation="MSAAANEEVSARSKLLKLTRRRLEKYATLLPKLLVSDDPETVHH
                     LRVWSRRLQQVLRVIARSEQAHRIKKIMRVLRYVRRTLGPCRDLDVNIALLQSKREAN
                     GTGSVQRAWQAMQSELESQRQVLIEQVRRDIAGRDHFKFITQVQALIARAAERDYDPL
                     EQTTAAMITSMDRWDESFTAAREQPDAAALHALRIAGKKLRYRAELLAALGQPKVNPL
                     VKVLKEIQKTLGDWHDRSVLLHHVAAFIGRPAFLADHPDIGRILLAEMEKERLRNDSD
                     VAIVLEQAAKVREAWINLKTRVAAV"
     gene            complement(3259..3387)
                     /locus_tag="VE170_08110"
     CDS             complement(3259..3387)
                     /locus_tag="VE170_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15438.1"
                     /translation="MTRVLQSGGVDYWESGMGPRMGEDLYESFIGYQKCCWPSLTR"
     gene            complement(3384..3515)
                     /locus_tag="VE170_08115"
     CDS             complement(3384..3515)
                     /locus_tag="VE170_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15439.1"
                     /translation="MEFFAAWAWVIVRSMDPEWLREVYSFALKPDAIFVCGSTSTTW"
     gene            3576..3701
                     /locus_tag="VE170_08120"
     CDS             3576..3701
                     /locus_tag="VE170_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15440.1"
                     /translation="MLVPSHHPFDDKQPPKKAVFAVHLHPYGHVNDPRFFTIYIA"
     gene            3976..>4511
                     /locus_tag="VE170_08125"
     CDS             3976..>4511
                     /locus_tag="VE170_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015215180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYJ15441.1"
                     /translation="MLALVGIVPRPAKAQIVQIDGSSTVFPVTEAVAEEFQKLKKGKV
                     KVTVGIAGTGGGFKKFCRAETDISNASRPILKSEIEDCKKSGVQFIELPVAYDALTVI
                     VNPKNDWVKSLNVPDLKKMWEPAAQGKVSSWNQVRSEWPNNPLKLFGPGADSGTFDYF
                     TEAIVGKAKSSRGDFTAS"
BASE COUNT         1127 a         1308 c         1165 g          911 t
ORIGIN      
        1 aggaagaagc ccggctcatc agtcttggca tcatgagcgg cctgcgcttt catttaccgc
       61 tcggcctttt cctcgacatc ggcggcggca gcgtcgagct tgccgtcgcc aacaccacca
      121 acacctattg cgtgttcagc ctgcccttag gcgccgtgcg cttgaccgag aagctggtcg
      181 cccatgaccc gccccgcaac aaagaaatca aagagctgcg cgagtacgtc caggcacggc
      241 tcaaacccgt tgtgcggcgt atccacaaag aaaaatatac catggcattt ggcagcggcg
      301 gcacgatcac cgccctcgcc gagaccgata cccgcgtaac cggcgatccc aatcgtgccg
      361 gatcgttgat cgtgctgcgg cgccagcgct tacgagcgct gttggaaatg ctgatgaacg
      421 agcccgccga cgagcgcgcg aaaatgatca gcggcgatcc caagcgcgcc gatattatta
      481 tcgccggcgg cttcgtgctg cacgagatca tgacggagat cggcctggaa tatattttcg
      541 tgtcgcgcag gggcctgcgc gacggcctga tggtcgatct gctgcaaaaa cgccattcgg
      601 aaagcggccc atggcatccg gacgctgatc gtgcggagtc tttggagcaa gtctgtcaaa
      661 aatatttgta tgacgccgca cacgcgcacc atgtcagcca attagcgctg aatctattct
      721 accaactgca cgacttgcat ggattgcccg agaattatgc gagcattctc catgccgccg
      781 ccatgctgca cgatatcggc cagttcctcg ccggacccaa gcaccacaag cattcctact
      841 atctgatcaa gagctctggc atgaactcgt tcaaccatct cgatctcgat ctggtcgcca
      901 acgtggcgcg ctatcaccgc aaggcccacc ccagcccaaa gcatttggga ttcagccaac
      961 tatcaccgtc aaatcaagac atcgtccgca agctcagcgc cttggttcgc gtcgccgacg
     1021 catttgactt tagacacaac cagcaggtag ataacctgag ctgcacgttt aagaaaaccc
     1081 gcaccttgac tctatcggca gcgtcagccg ccaacctaaa ggaagaaatc cgctgggccg
     1141 gacaaaaagg taaacttatt caagaggttt tcaacgtcga tctaattatc gaaaccaaca
     1201 agccatcgcg tgcgcggtag ctaaattcct aaggagatga acatgcgtcg ggagattcat
     1261 aacagaaaca tcctttttct ttgcgaagac aacgcccaac tcagtcagat ggccgaggct
     1321 acagccaaac atttatcacc gccgaaaaca cgtattttca gtgccggcat caagcccggc
     1381 cggattccac cttcggtgat caaagcgatg aacgaaatcg gcatcccaat cgctgaccag
     1441 aaaagcaaaa gcgtagccga ggtgcccatc gacgaaatcg atctggtcgt cagcttcggc
     1501 gatgcccaca aacaatgcgc ctacctaccc agacgcacta agatcgaaaa ttggccggtc
     1561 gctgacgagc tccagagcaa gaaagccggc gccgaagagc tagacgccgt gcgcggcgca
     1621 cgcgacgata tcgaaaagcg agtttttgcg ctgttcatgg actattggcg caatgtcgca
     1681 taatcgcttg agccgaccga gccatgaatc tctatctcat gcgccacggt atcgcggtcg
     1741 ccgccgatga cctgggcgtt attcaagaca atgaacgacc gttgaccagc aaaggcagca
     1801 aacgcatccg ccgcgccgcg cgcggtatac gccgattggg catcgagttc gatgggattc
     1861 tcaccagccc gctcctgcgc gcccgccaaa ccgcggacct cgtggccacc gcgctcgacc
     1921 tgcaatcgcg tttggaagaa atcaccgaac tcgcgccgga atgttccgtg gagcaactga
     1981 tcatttgctt aactcggtat caggaccgcg accatctgtt gctggtcggc catcaaccgc
     2041 tgttggggca aacactggat ggcttgattg agccaacccc cggaacgaaa atccatttcg
     2101 atctcggcaa aggttcgctg tgccgagtcg aagttgaccg actgccggtg gacaagccgg
     2161 gaatcctgcg ttggctcatg acggccaaac agctgcgact cctggcgagc cgctcgacca
     2221 agaattgagc gcaccatgag cgccgccgca aacgaagaag tttcagcgcg atccaaactc
     2281 ctcaaactga cacgccggcg gctggagaag tacgctacac tgcttcctaa attacttgtc
     2341 agtgacgatc ctgaaacggt ccatcacctg cgcgtctgga gtcgccgttt gcagcaggtt
     2401 ttgcgcgtca tcgcgcggtc ggaacaagcc catagaatca aaaaaataat gcgcgtcctg
     2461 cgctatgtcc ggcgaacctt gggaccgtgc cgggacctcg atgttaatat cgccctactc
     2521 cagtccaagc gtgaagcgaa tggcacgggc agcgttcaaa gagcctggca agccatgcaa
     2581 agcgaattgg agtcacaacg gcaggtgttg attgaacaag tgcgtcggga tatcgccggg
     2641 cgagatcact tcaagttcat cacccaagtg caagctttga ttgcgcgcgc cgccgagcgc
     2701 gattacgatc cgctcgaaca aactaccgct gcgatgatca cctccatgga ccggtgggac
     2761 gaatccttta ccgcggcgcg ggagcagccg gacgccgcgg ctttgcatgc gctgcgcatc
     2821 gccggcaaga aattgcgcta ccgcgctgag cttttggccg ccctcggcca acctaaagta
     2881 aatccattgg tcaaagtgct caaagaaatc cagaaaacct tgggcgattg gcatgaccgc
     2941 tcggtgctgc tgcatcatgt ggccgcgttt atcgggcggc cggccttttt ggcggatcat
     3001 cccgacatcg gccggatatt gttggcggag atggaaaaag aacggttacg taacgacagc
     3061 gacgtggcga tcgtattgga acaggccgcc aaagtgcgcg aagcttggat taatttgaaa
     3121 accagggtag cggcggtttg agggttttaa gaacgctaca tgcgattatc agcgataacc
     3181 gtatcgacga gctcgcacaa atctgtgaag acgtcttcct ggcagaagca tcgatgattt
     3241 tgaaaccgca agaaccaatc atcttgtcaa actcggccaa cagcatttct gatagccgat
     3301 gaagctctcg tagagatcct cgcccatgcg cgggcccatg ccggactccc aataatcgac
     3361 cccaccgctc tgtaaaacgc gtgtcaccaa gtcgtcgatg ttgacccgca aacaaaaatc
     3421 gcgtcgggct tcaaggcaaa gctgtaaacc tcgcgcagcc actctgggtc catgctgcgc
     3481 acgatcaccc atgcccaagc ggcgaagaat tccattcgct gaaataaacg acataaccct
     3541 ccgactcgag ccattttcta agcagaaaaa gttgcgtgct agtgccgagc catcatccat
     3601 tcgacgataa acagccgccg aagaaggcgg tgtttgccgt acatcttcat ccctatggtc
     3661 atgtgaatga tccaagattc ttcaccattt acatcgccta attcggcaga atctaacgga
     3721 tttactacgc tcgggcggct cttaaccatt tcttaacact atcgttacat ccacgatact
     3781 tagcgacttt accttcggcg tcgaatcgat acgatgaaaa taatttagcg ttaccttttg
     3841 gaagttgatt gagcagcaaa aagtctttaa ccgaattaac gcgtcgttaa agtggttgta
     3901 acacaaccgt aacattggct ccctaaataa ctggagagga ataaacatat gaagaaagca
     3961 ttactggcca tatccatgct cgccctggtt ggcatcgttc cgcggccagc caaggcgcaa
     4021 attgtccaga tcgacggttc gagcactgtg tttccggtca ccgaagctgt cgccgaagag
     4081 ttccagaagt taaaaaaagg taaggtcaaa gtcactgtcg gcatcgccgg caccggcggc
     4141 ggcttcaaga agttctgccg cgccgagacc gacattagca acgcttcccg gccgattctc
     4201 aaatccgaaa tcgaggattg caagaaaagc ggcgtgcaat tcatcgaact gccagttgcc
     4261 tatgacgcgc tcacggtcat cgtcaatccg aaaaatgact gggtaaaatc tcttaacgtt
     4321 ccggatctca agaaaatgtg ggagccggcg gcgcaaggta aggtttcatc ctggaatcaa
     4381 gtgcggtccg agtggcccaa caatccgctg aagctattcg gtccaggagc tgactccggc
     4441 accttcgatt acttcaccga agccatcgtc ggtaaagcca agtcgagccg cggcgatttt
     4501 accgccagtg a
//
LOCUS       DASQKS010000312         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2269445, whole genome shotgun sequence.
ACCESSION   DASQKS010000312 DASQKS010000000
VERSION     DASQKS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269445"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VE170_08130"
     CDS             complement(<1..1630)
                     /locus_tag="VE170_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYJ15442.1"
                     /translation="MITADRTVSTTCPYCGVGCGLQLHLKDDYIYKVTSSFDSVVNHG
                     NLCVKGRFGYDFVYNKDRVTTPLIRKRAQAPGARSQAFERDQWREVSWDEALDYSTER
                     LCAIYSRDGADAMAVYCCAKATNEDNYLLQKMYRALFRSNNIDHCTRLCHAGSVVALQ
                     MALGSSAMSNTAAEVIHSDLFFVTGSNTAETHPIIALQMKAAVEKHGAKLIVVDPRRV
                     EMVNWATLWLPEKPGTDVPVYSAMAHVIVKERLYNQDFIDRRTEGFPGFAKSIEKFTP
                     EYAEAISGVDRNLIIQAARMYAGAKNAAIYWALGIPEHSHGTDNAMTLIHLALLTGHI
                     GRPGTGLNPLRGQNNVQGASDSGAMPWHYPGYQRVDDEAAARKFEQAWHSDPGTLNRQ
                     PGLTTTEIMSAVGPGGVRALHIMGENPMMSEPNLNHTRHMMEQLEFLVAQDLFINESG
                     AFADVFLPAASWAEKEGTFTNTDRRVQRVRKALEPRGQSRADWQIICDLAGRIEKRLG
                     RGQSAVWSYQRPSEVLDEMAGVVPEYAGVRYSRIE"
     gene            complement(1627..2328)
                     /locus_tag="VE170_08135"
     CDS             complement(1627..2328)
                     /locus_tag="VE170_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ15443.1"
                     /translation="MPDVTLTIDDTSVTVAAGTTILKAAEQVGQRVPTICYHDHCTAN
                     GLCRICVVEVDGVRPLVPACLSPVSEGMKVSTSSARAARSRRTILEMLDSTVDLSQAP
                     DIQQLMDENHADRGRFPDAERREHALLDDNPMYIRDYSKCILCWRCVQVCADDAQFTY
                     AINFKGRGFDTGIGTFFDKPMADTTCVFCGQCVGVCPTGALKSKRESLLEQGFSLDEI
                     LQATRGGKKRRRKSP"
     gene            complement(2418..>2672)
                     /locus_tag="VE170_08140"
     CDS             complement(2418..>2672)
                     /locus_tag="VE170_08140"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYJ15444.1"
                     /translation="LKRLAHFFADESCGKCYPCQLGARRQYEILERTAAGKALTGDPE
                     RLADVGSTMIDASLCGLGQTAPTAVSSAMKLWPGMFEKRS"
BASE COUNT          508 a          792 c          807 g          565 t
ORIGIN      
        1 gttcgatgcg cgaatacctt acgccggcgt attcgggaac gacaccagcc atctcgtcga
       61 gtacttcgga cggtcgttgg tatgaccaga cggctgactg accgcgcccg aggcgctttt
      121 cgatgcgccc ggcaagatcg cagatgatct gccaatcggc gcgggattgg ccgcgcggtt
      181 ccagggcctt gcgcactcgc tggacgcggc ggtcggtatt ggtaaacgtg ccttctttct
      241 cggcccagga agcggccggc aaaaaaacgt cggcgaaggc gccggactcg ttgatgaaca
      301 gatcctgcgc caccaggaat tccaactgct ccatcatgtg ccgggtgtgg ttcaagttgg
      361 gctcggacat catcggattt tcgcccatga tgtgcagtgc gcgcacgccg cccggtccca
      421 ccgcgctcat gatttctgtc gtcgtcagac ccggttggcg attgagcgtg ccggggtcgc
      481 tatgccatgc ctgttcgaat ttgcgcgccg ccgcttcgtc atcgacgcgc tgatacccgg
      541 gataatgcca gggcatggcg ccggagtccg aagcaccctg gacgttgttt tgcccgcgca
      601 gcggattcag accggtgcct ggacggccaa tgtggccggt gagcagcgcc agatggatga
      661 gcgtcatggc attgtcagtg ccgtgggagt gctcgggaat ccccagcgcc caataaatcg
      721 ccgcgttctt tgcgccggca tacatgcgcg ccgcctgaat tatcaaatta cggtcgacgc
      781 cggaaatcgc ctcggcatat tcgggcgtga acttctcgat cgatttcgca aaaccaggga
      841 agccttcggt gcgccgatcg atgaaatcct gattgtagag ccgctccttg acgatgacat
      901 gcgccatcgc cgaataaacc ggcacgtcgg tgcccggctt ctcgggcaac cacagcgtcg
      961 cccagttgac catctccacc cgcctcggat cgacgacgat gagcttggcg ccgtgctttt
     1021 cgaccgcggc tttcatttgc agggcgatga tcggatgggt ttcggcggtg ttcgagccag
     1081 tgacgaaaaa gagatcgcta tgaatgactt ccgcggcggt gttgctcatc gccgacgagc
     1141 cgagcgccat ttgcaaggcg accaccgagc cggcatggca taaacgggtg cagtggtcga
     1201 tgttattgct gcggaacagc gcgcggtaca ttttctgtaa aaggtagttg tcctcgttgg
     1261 tcgccttggc gcaacagtaa accgccatgg catcggcgcc gtcgcggctg taaatcgcgc
     1321 atagccgctc ggtgctataa tcgagggcct catcccacga aacttcgcgc cactgatcgc
     1381 gctcgaaggc ttggctgcgc gcgcccggtg cctgtgccct cttgcgtatg agcggcgtgg
     1441 ttacccgatc cttgttgtaa acgaagtcgt agccgaagcg gcccttaacg cagagattac
     1501 cgtggttgac gaccgagtcg aaggatgaag tcactttgta gatgtagtcg tcctttaaat
     1561 gaagttggag gccgcatccg acgccgcagt agggacaggt tgttgatact gtgcgatcgg
     1621 cggtgatcat ggcgatttcc tccggcgctt tttcccgcct cgggtagcct gcaggatttc
     1681 atccaaggaa aatccctgtt ccagcagcga ctcgcgtttg gacttgagcg cgccggtcgg
     1741 gcagacgccg acgcattggc cgcaaaatac gcaggtggta tcggccatgg gtttgtcgaa
     1801 gaaggtgccg atgccagtgt cgaagccgcg ccctttgaag ttaatcgcgt aggtgaactg
     1861 cgcgtcgtcg gcgcagacct gcacgcaacg ccagcacaga atgcatttgg aataatcgcg
     1921 gatatacatg gggttgtcat cgagcagcgc atgctcgcgc cgttcggcgt cgggaaaacg
     1981 cccccggtcg gcgtgatttt cgtccatcaa ttgttggata tccggcgcct gcgaaaggtc
     2041 caccgtcgag tcgagcatct ccaaaatggt gcggcggctg cgcgcggcgc gcgcgctcga
     2101 cgtcgagact ttcatgcctt cactcaccgg gctcaagcat gccggcacga ggggcctgac
     2161 gccgtcgacc tcgaccacac aaatgcggca tagcccgttc gccgtgcagt gatcgtgata
     2221 gcatatggtc ggaacccgct ggccaacctg ctcggcggct ttaagaatag tcgtccccgc
     2281 ggctacggtg acggaagtgt cgtcaatggt gagagttaca tcaggcataa aaattgcccc
     2341 tggagtactg gaggattgga ctgatgtttt ccggacccag tattccaata ctccatcact
     2401 ccggctcgtg tttactccta gcttcgcttt tcaaacatcc ccggccacaa cttcatcgcg
     2461 ctcgacaccg ccgtgggcgc cgtttgcccc aggccgcata gcgacgcgtc gatcatcgtc
     2521 gaaccgacat ccgccaagcg ttcgggatca ccggtaagcg ctttgcctgc cgcggtccgt
     2581 tcaagaattt catactgccg gcgcgcgccc aactgacagg gataacattt gccgcagctt
     2641 tcgtcggcga agaagtgcgc cagccgtttc ag
//
LOCUS       DASQKS010000313         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1833500, whole genome shotgun sequence.
ACCESSION   DASQKS010000313 DASQKS010000000
VERSION     DASQKS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833500"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VE170_08145"
     CDS             complement(<1..215)
                     /locus_tag="VE170_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ15445.1"
                     /translation="MQFKIKPATKKDVPVILSFIKKLADYERLSHEVVATEELLQRTL
                     FGQRRTAEVAIGYYDDAPVGFVLFFHN"
     gene            complement(547..2763)
                     /locus_tag="VE170_08150"
     CDS             complement(547..2763)
                     /locus_tag="VE170_08150"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ15446.1"
                     /translation="MAKREFKFVGHNVKRVDGVEKVTGAAKFVGDITVPGMLYGKILR
                     STYPHARIRSIDASQAEALPGVIAVLTAADIADLNAIYNGRPVIAINKVRYVGEPVAA
                     VAAVNIAIAEEALALIQVDYEELPTVSHIEAAIRPGAPLVHEDKRNNIGTHEKVERGN
                     VDEGFAQSDMIAEDNFTFPMVYHYAMEPHSAIAHYDVNDEITVWSCAQHPFQVRGDIA
                     KVFKVPTAKVRLIINFLGGGYGSKSYTKFEPLVVALARKAKQPVRICNSVGESMVTVR
                     RHGARVRVKTGVKNDGTIMAREAEIYLDTGGYDDNGPQVTARSATRILGPYWIPNIRT
                     NAYQVYTNTGSAGSYRAIGAPQVIFAGESQIEILAAKLGMDAAALRAKNLLKRGQELR
                     PGLKGIDANLSASLKTLMRASHWKRSQMNKNSAVGMGCAITNAGATPVSVAMIRMQSD
                     GVANIAAGSTEMGQGVRTTLTQIAAEELTLPMEQFRMLGADTKVTPYDSSTGSSRSTT
                     LMGTAVQKAALDLRRQFVQIAAQTMGVKAKQIQVIDGALVCGESRLTFKEAFEKRFGK
                     GSGGEMIGCGEAGPDITDNQLPVFWEVGMGTAEVDIDRETGAVNLIKFISVADVGKAI
                     HPEHCVAQEEGAAMQGIGHTFFEQLIYENGQLLNPNLVDYRIPSFADVPAEFHTELVE
                     NGDGPGPFGVRGMAEGGILSVAPSVCNAIDRATGVRIRDLPLTPERVWRALKDAVK"
     gene            2881..>3439
                     /gene="sfsA"
                     /locus_tag="VE170_08155"
     CDS             2881..>3439
                     /gene="sfsA"
                     /locus_tag="VE170_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HYJ15447.1"
                     /translation="MGFISCFGYKTPMRFKSRLIRGTLIQRYKRFLADVRLANDEIVV
                     AHCTNTGSMMGCKEPGSAVYISRSDNLNRKLAYTWEMIEIGGRWVGINTMHPNKLVAE
                     AIESGVIEELSGYSVIRREVKVSAHSRLDLCLESRGGNCYVEVKNVTLTVDGSAAFPD
                     AVSERGTKHLKELIRLKRQGHRAALV"
BASE COUNT          702 a         1040 c          952 g          745 t
ORIGIN      
        1 aaattatgaa agaacaagac aaagccaacc ggcgcgtcat cgtagtagcc gatcgccact
       61 tccgcggtgc gccgctggcc gaacagggtg cgctgcaaga gctcctcggt cgcgacgact
      121 tcatgggaca aacgttcgta atcggcgagc ttttttataa atgagagaat taccggcaca
      181 tctttcttgg tcgccggctt tatcttgaac tgcatgagtc ccgtcgtaat cgtcagaggt
      241 cggtcctaga gtgaaactat tcgctacgaa actatgagcg cggcagagcc gccaccaaaa
      301 cccgaaatac cccgcgcaca tcacccagag ggcgccgcga acagttaaaa taaatttcca
      361 actctatgcc cttaccggtc ttgggacgca atgatccgag tctagtcggt gcgcggacga
      421 acccggcgaa aatgttcgtg tccttcgtgg cgcctttgca atagccggcc acggactgtg
      481 cggcgtgggt ttgatcatga ttcactttgt ggtgggtggc cttttttctt attcgttaga
      541 cccaaactat ttaaccgcgt ctttgagcgc gcgccacact cgttccggtg tcagcggcag
      601 atctcgaatg cgcacgccgg tggcgcggtc gatggcattg cacaccgacg gcgcaaccga
      661 gagaattccg ccctccgcca tgccgcgcac gccgaagggt cctgggccgt ccccgttctc
      721 gaccagttcg gtatgaaatt ctgccgggac atcggcaaac gatggaatgc ggtagtcgac
      781 cagattggga ttgagcaact ggccgttttc gtaaatcagc tgttcgaaga acgtatgacc
      841 gataccctgc attgccgcgc cttcttcctg ggcgacgcaa tgttccgggt gaatcgcctt
      901 gccgacatcg gcgacggaaa tgaactttat caaattcact gcgccggtct cgcggtcgat
      961 atcgacctcg gcagtaccca tgccgacttc ccagaatacc ggcagttgat tgtcggtaat
     1021 gtccgggccg gcttcgccgc aaccgatcat ttcaccaccc gagcctttgc cgaaacgttt
     1081 ctcgaacgct tctttgaacg tcagccgcga ctcgccgcac actagcgcac cgtcgatgac
     1141 ttgaatctgt ttcgctttta cgcccatggt ctgggcggcg atttggacaa attgccggcg
     1201 caaatcaagc gctgcttttt gtaccgccgt gcccataagc gtagtcgaac gactcgagcc
     1261 cgtcgaacta tcgtaaggtg taaccttggt atccgcgccg agcatgcgaa actgctccat
     1321 tggcaaggtc aactcctcgg cggcgatctg cgtcaaggtc gtgcgcacgc cctggcccat
     1381 ctccgtgctg ccggcggcga tattggcgac gccgtcggac tgcatgcgaa tcatcgctac
     1441 cgacaccggc gtcgcgccgg cgttggtgat cgcgcagccc atgccgaccg cgctattttt
     1501 attcatttgc gatcgcttcc agtggctggc gcgcatcaag gtttttagac tcgcggaaag
     1561 attggcgtcg atccccttca atccggggcg cagctcttgg cctcgcttga gtaagttctt
     1621 tgcccgcaac gccgccgcgt ccatacccag cttggcggca agtatttcga tttgcgactc
     1681 gccggcgaaa atcacctgcg gcgccccgat ggcgcggtag gagcccgccg agccggtgtt
     1741 ggtatagact tgataggcat tggtgcggat gttgggaatc caatagggac cgagaatgcg
     1801 cgtcgccgac cgcgccgtca cctgcggccc attatcgtca tagccgccgg tgtcgagata
     1861 aatctccgcc tcgcgcgcca tgatcgtgcc gtcgttcttg acgccggtct tgacccgcac
     1921 ccgggcgccg tgacggcgca ccgtgaccat cgactcgccg acggaattgc aaatccgcac
     1981 cggctgtttg gccttgcgcg ccagcgcgac gaccagcggc tcgaacttgg tgtacgactt
     2041 actgccgtag ccgccgccaa ggaagttgat aatcaggcgc accttcgccg tcggaacttt
     2101 aaagactttg gcaatatcgc cgcgcacctg aaatggatgc tgggcgcaag accagacggt
     2161 aatctcgtcg ttgacatcgt agtgcgcgat cgccgagtgc ggctccatcg cataatgata
     2221 gaccatcggg aaagtgaagt tgtcctcggc gatcatgtcc gactgcgcga acccttcgtc
     2281 gacattgccg cgttcgacct tttcatgggt tccgatgttg tttcgtttgt cttcatgcac
     2341 cagcggcgcg cccggcctga tcgcggcttc gatatggctc acagtcggca gctcttcata
     2401 gtcgacctga atcagcgcca gcgcttcctc ggcaatggca atgttcaccg ccgccaccgc
     2461 cgccaccggc tcaccgacat agcgcacctt gttgatggca atcaccggcc ggccgttgta
     2521 aatcgcgttc agatctgcga tatccgccgc cgtcaacacg gcgatgacac cgggcagggc
     2581 ttcggcttgc gaggcgtcga tcgagcggat ccgcgcatgg ggataagtgc tccgcaagat
     2641 ctttccgtag agcatgccgg gaaccgtgat atcgccgaca aatttcgccg cgccggtcac
     2701 cttctcgacg ccgtcgaccc gtttgacgtt gtgcccgaca aatttgaact ctcgcttggc
     2761 catgaattct ccgtaaaaaa actgatgtga gattcctaac aaagcgcttt cgcttgttca
     2821 aggaagtgcg aagcactcgc ctggcatttc gactgaggga gaaatctttc cggcgccacg
     2881 atgggattta tcagctgctt cggttataag actccgatgc gcttcaaatc acgcctgatc
     2941 cgcggcaccc tgattcagcg ctacaaacgt tttctcgccg atgtgcgttt ggcaaacgat
     3001 gaaatcgtcg tcgcccattg caccaatacg ggcagcatga tgggctgcaa agaaccgggc
     3061 agcgcggttt acatttcgcg cagcgacaat ttgaaccgta aactcgccta cacctgggaa
     3121 atgatcgaaa tcggcggccg ctgggtcggg atcaatacca tgcacccgaa caaactcgta
     3181 gcggaagcga ttgagtcggg ggtcatcgaa gaactcagcg gctactcggt tattcgccgc
     3241 gaggtcaagg tcagcgccca ttcaagactc gacttgtgcc tggaaagtcg tggcggcaac
     3301 tgttacgtcg aagtgaaaaa cgtcactctt accgtcgacg gcagcgcggc ctttcccgac
     3361 gccgtaagcg aacggggcac caaacacttg aaagaactca tccgtctcaa gcgccagggc
     3421 caccgcgctg ccctcgtct
//
LOCUS       DASQKS010000314        11483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5192973, whole genome shotgun sequence.
ACCESSION   DASQKS010000314 DASQKS010000000
VERSION     DASQKS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11483
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5192973"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VE170_08160"
     CDS             <1..706
                     /locus_tag="VE170_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15448.1"
                     /translation="KRIGVGEYQQTAALWQRAILEHDFGVSQYKVDWYMERTEAMSHG
                     GVTGFTPPEGISFHRVPSDKSLASMLVNKELDVAAINSPWKNAPSQLGRSHQITGAGG
                     DWSKVKMLFPDRVAEGKRFFNKWGFLPVNHAYTIRGDIYRKHPWVAFNLYSAFVKAKA
                     HFNAKLVDSIPSGLFFGKEYLTKTQEIFGSDPFPYGVKANRKLLETLIDFSHEQGLIT
                     KKPKIEELFAVSTRDL"
     gene            889..1863
                     /locus_tag="VE170_08165"
     CDS             889..1863
                     /locus_tag="VE170_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15449.1"
                     /translation="MKRPLVTSLLASVFLQVLGSATYAASLKSINFASNTPTLSGTLP
                     LVVAQEFGFFAAEGLDVKTVLIRGGPTAMAALVGGGVDYTLVAGVAAVRAIAQNAPMT
                     IISGMQPYMDYTLLGAKGITGVNDLKGKVVGVTGPGGIAEFATVEGLARKGLVRDRDY
                     KILYGVGNSPARAQALETGKIQASPFSFLERLELERKGYPTLFDIGSVMPGFPFVVIV
                     TSKQKIDTDPEGIVSMLRAIKRGLDFLAGNHEKVAAVVIRKSHFGDPATVRKVIAQFA
                     EVYSVAIAKEDIEALIAATRIEAEAKKLGGSDKFFTRQFLIKALGQSR"
     gene            1875..2828
                     /locus_tag="VE170_08170"
     CDS             1875..2828
                     /locus_tag="VE170_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15450.1"
                     /translation="MAQDIELSLLLADYHRTNPILSGAVTAEGIKFASQQAIPGEACM
                     RPVYEEYDIAEMSLSWYVMARSRNEPVIALPIFPLRMQIHPYIFCSPSAGIERPEDLK
                     GKRIGMDQYRLTVGLWARGILQEYHGVRPQDCQWFTSEPESPDAGFQTPEGVNITLAN
                     QSTEAMLLRGELDALIPPNIVPSFRNKDRRIRRVFQDARATVNEYFRKTKIFPITHTL
                     VVRQELFDRQPWLVASLLKTFDEAESLCRKSYEYAKRSAFPSAVLILEEEEEVFGKNP
                     WAHGLTPENRVVLEKFVEYANQQGYIPNRPALSELFAPVGN"
     gene            complement(3218..3439)
                     /locus_tag="VE170_08175"
     CDS             complement(3218..3439)
                     /locus_tag="VE170_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15451.1"
                     /translation="MTEFERAENYRIETSLYERAPGRWTVAAEIFFKTVPAGDARVIS
                     RMMKSDFTTKDDAEAAALGWAKKEINDFD"
     gene            complement(3556..4482)
                     /locus_tag="VE170_08180"
     CDS             complement(3556..4482)
                     /locus_tag="VE170_08180"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ15452.1"
                     /translation="MSLKLSGILSANVTPFKENYDLDEAGLRKLIRYQAKPLGINAIV
                     CNAGAGEGATLTREERVRCIEIIREEIRPEQHVVAGIEALSTREAIRQTQEAKAAGAA
                     AIMLTPPHVYDWDSRTNPEFAVQYFHDIAKAVDIPITIFHYPAAVTSGYTPDTAIRIA
                     REVDNVAAIKVASGANIRRYEQVLRGMRGLPRHVAVLSTSSLFQHFVTGGDGALTGFA
                     NFAPDFCCDLFKAVSDGKLDEARRLHEINWALEAAVYKAPNVYKHSRYKVAAYFAGLM
                     DNFIVRAPQVPVPEGEVKLIHDAMAKLGMVQR"
     gene            complement(4650..5642)
                     /locus_tag="VE170_08185"
     CDS             complement(4650..5642)
                     /locus_tag="VE170_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15453.1"
                     /translation="MSLFRFVSILIAVFCSAPSLAASKAPTQLRVAYSSISGSAVIPW
                     IAVDKGIFAKYDLDVELIYVAGSPAMQSLLGGTTQLGIQGIEPVMRVNAQGSDAVMIV
                     GLVTKPPFSIIVRPEIREPKDLKGKAMGVTRFGSTTDALLRLALDKWGLRPEVDVPII
                     QMGGVPPILSGMQSKKIYGGPISLPTLAVAKQEGFRELVDLADVIPDYQAAGVVTRKL
                     FLRQNPETSRNFVRAVSEAIAVFKNDPAYSKKIIKDRLKIDDILVQEETQRTYLNYVP
                     RVPYPNRAGIALIKSFLEKSEPALRPLSVDEQIDGSLVRGLEQSGFFNGLYPAK"
     gene            complement(5675..6151)
                     /locus_tag="VE170_08190"
     CDS             complement(5675..6151)
                     /locus_tag="VE170_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15454.1"
                     /translation="MSLKLRRVVTGHDGNGRAIVKIDEVCKNLAAGRPGRSACVVWTT
                     ESFPVNNTGGADEGLRQVGTTLHNGTVFRVVEFAPGVAPRNHRTDSIDYAVVMSGEID
                     MELDDTVVHLQAGDVLVQRGTIHNWVNRGNEPCVIAFILIDAKPVDVGGKILHAEG"
     gene            complement(6389..7165)
                     /locus_tag="VE170_08195"
     CDS             complement(6389..7165)
                     /locus_tag="VE170_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ15455.1"
                     /translation="MIKEGKFTILETRGAVAKLTINRPEKRNALSRDTIREILAVFEE
                     LRNDDEIAVVLTTGAGDVAYCAGRDLSEFPTEGGQNRGKDRRKEPRAYHVAEVIRTFP
                     KVTIAVVNGFCLGGGITLLLPHDLAIASDKAMFGLPEIMRGFLPYPIIATMFKTLIPT
                     KLGFELILTGKNWDAVKSMNAGLINRVVPHAQLQDEAWQWAEEIGKFDKTTLKYCKMA
                     AHASMEAASVPMAAEIAWLMQEEHALVNPRAYAGTKQFHK"
     gene            7515..8363
                     /locus_tag="VE170_08200"
     CDS             7515..8363
                     /locus_tag="VE170_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ15456.1"
                     /translation="MESTIRRISKSVFAIIGPEGATNFTIIKGANGSAVLIDADIRRI
                     DEVEEALRLTGCAGVSYLIDTHEHFDHTSANFYFAQKQIPIVASGGCVQAMKDDGAAD
                     FARMLKPVPELYQRFPGLCLTLPDVVFSSTTQIKVPGVTLDLVYRAENGQSHSKGDTT
                     IYFHEEEVFIAGDLLYTEVHPVTFFGNIPNWLTALKPLMDYRYNQLIPGHGPAVEGEQ
                     SGRAYFKRMHDYLEDFYGRLVEIDSGRTSREEVARHMQSGAYAELGKTRMVERNINQF
                     LTGRWF"
     gene            8364..9341
                     /locus_tag="VE170_08205"
     CDS             8364..9341
                     /locus_tag="VE170_08205"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15457.1"
                     /translation="MKLTAVLLIALALIAAGNSLEAAEKLRAAYPAIAPGSTPSWVTA
                     ERGIWRKYGFDVEPILVSGGARAVPALIGNSVQFLIGSDTGVTTAQLQGIAIVRLGVT
                     MNTLGSSLLTQPSIQTVQDLKGKTLGISRGRDASYIRLAKLLRDNGLDPNQDVKFLSI
                     GGGEGGRVSALKAGVIHGTMLFPPLDLIARNDGLKVLSKFDVPTPGGGISTSAALLKQ
                     NRPLVLNFLKGYIEGIHYMIGHKPESLKILQRYFQNSDLTAMGYLYDETTRRLEKELR
                     ASPESIAFHYDMAAQDDPRAKQATEKTFWDNSLIEEIRRSGFIDQLYKN"
     gene            complement(9390..10373)
                     /locus_tag="VE170_08210"
     CDS             complement(9390..10373)
                     /locus_tag="VE170_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15458.1"
                     /translation="MKSLSIGFALVACFLVPTSKLWSADVLRVGYPSLATGFAASWVT
                     ADKGFWKKHGLDVELIFLRGGGRTVAALIGGSVDFILGSDLGITTGIIQGASLTRLGV
                     TTNTLGYSMIVQPGIKTVRDLKGKIIGITPGRDAAYARVVKLLRDNGMDAAKDVTFLS
                     VGDGGPAARVAALSSGVIHATMFTPPSDKISEKSGMSILSRLDVANVGGGINTTPAFV
                     QKNRPQALRFLRGYMEGIHYLKANRSESLKIFAKYVRNPDLEIMAYLYEEISTRAEKD
                     LRPQSDAVRALLDLVALDFPQAKKLTEKDNWDLSLIDEIQRSGFLEQLYRK"
     gene            10803..>11483
                     /locus_tag="VE170_08215"
     CDS             10803..>11483
                     /locus_tag="VE170_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ15459.1"
                     /translation="MNIGESLTRNAQHFPDKRAIVDAHRAVTFGELHERTDRLANYML
                     DLGVGKGDLVGLSVGSRTEHFEALFAIAKIGAMAIPFDFNWSAQECESMLRFLAPKAL
                     FVEIRKETEPLLELAREFIPAEKLILIAADNCQGGVGMESIIECGNPSEPTTFVEGKD
                     PFLLMITSGTTGFPKACSIDHQTYALRCLNYSVSKGMNHNERALMALPVHFNAGRGSV
                     MAILYLGGT"
BASE COUNT         2562 a         3434 c         3054 g         2433 t
ORIGIN      
        1 caagcgcatc ggcgtcggtg aataccagca aaccgcggcg ctctggcagc gcgctatact
       61 cgagcacgac tttggagtat cgcagtataa agtcgattgg tacatggagc gcaccgaggc
      121 catgagccat ggcggcgtca ccggtttcac gccgccggaa ggcatctcgt tccaccgggt
      181 gccatcagac aagagcctcg cgtcgatgct ggtgaataag gaattggacg tggcggcgat
      241 caacagtccc tggaaaaacg cgccgagcca gctcggccgc tcgcaccaga tcaccggcgc
      301 aggcggcgat tggagtaaag tgaagatgct gttcccggac cgcgttgccg aaggtaaacg
      361 gtttttcaac aagtggggat ttctcccggt gaaccatgcc tacacgattc gcggcgatat
      421 ctatcgaaaa cacccctggg tcgctttcaa cttgtacagc gcctttgtca aagccaaggc
      481 gcacttcaac gctaagttgg tcgacagcat tccttcaggg ctgttctttg gcaaggaata
      541 tctgaccaag actcaagaga tattcggcag cgaccccttc ccctacggcg tcaaagccaa
      601 tcggaaattg ctcgagaccc tcatcgactt ttcccacgaa caaggcctga taacgaaaaa
      661 gcccaagatc gaagaactat tcgccgtaag cacgcgggat ttgtgagggg aatgtaagtg
      721 aagcggagca gtgctggcgt ttaggttcgg ctacgaaatg accagatccg cttgaacttg
      781 aaccgcccgc ggcggacaat ccgcaaaccg ctcggtgaag cttgagagtt cgtcaaacat
      841 tcggaacaac aagaacgagg acagctcccc gaaagactaa actaacccat gaaaagacca
      901 cttgtcacct cgctcctcgc cagcgtcttc ttgcaagtcc taggcagcgc cacttatgcg
      961 gcatcgttga aatcgattaa tttcgcgtcg aacacgccga ccttgagcgg cacgttgccg
     1021 ctggtcgttg cccaggagtt cggttttttc gccgccgaag gtttggacgt aaaaaccgtg
     1081 ctgattcgcg gcggaccgac ggccatggcg gctctggtcg gcggcggcgt cgattacacg
     1141 ctggtggccg gtgtggcagc ggtacgcgcc atcgcgcaaa acgcgcccat gacgatcatc
     1201 agcggcatgc aaccttacat ggactacacg ttgctcggcg ccaaaggcat caccggcgtc
     1261 aacgacctca aaggcaaagt cgttggcgtc accggtccgg gcggcatcgc cgagttcgcc
     1321 acggtcgaag gactggcgag gaagggttta gtgcgcgacc gcgattacaa aatcctctac
     1381 ggcgtcggca acagtccggc gcgcgcgcaa gcgttagaaa ccggcaagat ccaggcctcg
     1441 ccattttcct tcttggaaag attggagctg gaaaggaaag gttatcccac gctcttcgat
     1501 atcggcagcg tcatgccagg atttcccttc gtagtgatcg ttaccagcaa acaaaaaatc
     1561 gacaccgatc ctgaaggaat cgtttctatg ctgcgggcaa tcaagcgcgg cttggacttc
     1621 ctcgctggca accatgaaaa agtcgccgcc gtggtgatca ggaagagtca cttcggcgac
     1681 ccggccacgg tgcgtaaagt catcgcccag tttgccgagg tctattcggt ggcgatcgcc
     1741 aaggaagaca tcgaagcgtt gatcgccgcc acgcgcatcg aagcggaagc gaaaaagctt
     1801 ggcggatcgg acaaattctt tacccggcaa tttctcatca aagccctggg ccaaagccgt
     1861 tgaaaggaac catcatggca caagatatcg aactgagtct cctgctcgcc gactatcacc
     1921 gcaccaaccc gatcctctcc ggcgcggtca ccgccgaagg tatcaagttt gcctcgcaac
     1981 aagccattcc aggcgaggct tgcatgcggc cggtttatga agaatacgac atcgccgaga
     2041 tgtcactttc ttggtacgtg atggcgcgca gccgcaacga gccggtgatc gccctaccga
     2101 tttttccgct gcgcatgcag atccatccgt atatcttttg ttcgccgtca gccggcatcg
     2161 agcggcctga agacttgaag ggtaaaagaa tcggcatgga tcagtaccgc ctcaccgtcg
     2221 gcctgtgggc ccgagggatt ttgcaggagt atcatggcgt gcggccccag gattgccaat
     2281 ggttcacctc agagccggag tcgccggacg ccggctttca aaccccggag ggcgtcaaca
     2341 tcaccttggc gaatcagtcg acggaagcga tgctcctgcg cggcgagctc gatgccttga
     2401 tcccgcccaa catcgtgccg tcattccgca acaaggatcg gcgcattcgg cgcgtcttcc
     2461 aggacgcccg cgccaccgtc aacgaatact tccgcaagac taaaatcttc ccgatcaccc
     2521 acaccctcgt ggtgcgccaa gaattgttcg accggcagcc ctggctggtg gcgagcttgc
     2581 tgaaaacgtt tgacgaagct gaatcgcttt gccgcaaatc ctatgaatac gccaagcgct
     2641 cggcctttcc ttctgccgtc ctcattttgg aagaagagga agaagtcttc ggcaagaatc
     2701 cctgggctca cggcttaacg ccggaaaacc gagtggtgct ggaaaaattc gtcgagtacg
     2761 ccaaccagca gggttacatc cccaaccggc cggcgctgag cgagttgttc gccccggtcg
     2821 gcaactaacg tcgagaggga gcacaggaca gcaccgcccc aaggcgcatg ccatgcgaca
     2881 gggacacgga ttcgatagcc gagcgcctca aaacagaaag cgccctgacg tggagtaaat
     2941 cgaacgagcc cgacccggct tgtgttgttt ggtttatgaa actttggact tagacgtggc
     3001 cggctttagt tcgggctggt tgttctgctc ctaaggatag cggcagcttc gtcgatatca
     3061 taaccggcaa cactgtgatc gccggcattg agcaactcac gagtcaggcc gccaacgccg
     3121 cacaagaagt ttaccgcgac agctgttttt tcttcagttt attgacttgt gaagaggcca
     3181 caaattacaa gtatttctat ggccttagca gtctatttta atcgaaatca ttaatttctt
     3241 tttttgccca tccgagggcg gcggcctctg cgtcgtcctt tgtggtgaaa tcgcttttca
     3301 tcatccgaga tataacccgc gcatcgcctg cgggcacagt cttgaaaaag atctcagcgg
     3361 cgacagtcca gcggcccggc gcgcgttcgt acaacgaggt ctcaatacga taattttccg
     3421 ccctttcgaa ttcggtcatt aaaggaactc ctttccccat ttgatccaat tctaagctac
     3481 ccgcctgaaa ggcgtcaatc ggtgagacac cctaagagcg cgtgacaaag aacccagccg
     3541 tgaattggtc gccacttatc gctgcaccat gcccaacttc gccatcgcgt catgaatcaa
     3601 cttcacttct ccctcgggca ccggcacctg cggcgcgcgc acgatgaagt tatccattaa
     3661 gccggcaaaa taagccgcca ctttgtagcg gctatgtttg taaacgttgg gtgctttgta
     3721 gaccgcggct tccagcgccc aattgatttc gtgcagccga cgcgcctcgt cgagcttgcc
     3781 gtcgctcacc gctttgaaaa ggtcacaaca aaaatccggc gcgaagttgg caaagccggt
     3841 caacgcgcca tcgccgccgg tgacgaagtg ttggaatagc gacgacgtgg acaataccgc
     3901 gacatggcgc ggcagcccgc gcatgccgcg cagcacctgc tcgtaacggc ggatgttggc
     3961 gccgctggcg actttgatcg cggcgacgtt gtcgacttcc cgggcgatac ggatcgccgt
     4021 gtcgggcgtg tagccggaag tcaccgccgc tggatagtga aagatggtaa tcgggatatc
     4081 gaccgctttg gcgatatcgt ggaaatactg cacggcaaac tccggattgg tccgcgagtc
     4141 ccagtcataa acatggggcg gcgtgagcat gatcgccgcc gctccggccg ccttggcttc
     4201 ctgcgtctgt cgaatcgcct cccgcgtcga cagcgcttcg ataccggcaa ccacatgctg
     4261 ctcgggacga atctcctcgc ggataatctc gatacaacgt acccgctctt cgcgtgtgag
     4321 cgtcgcgccc tcgccggcgc cggcgttgca cacgatcgcg ttgatcccca gcggtttagc
     4381 ctgatagcga atcagcttgc gcaaccccgc ttcgtccaga tcgtagtttt ctttgaacgg
     4441 cgtcacgttg gcggaaagaa tcccggatag ttttagactc atggcaacct cgatacagaa
     4501 tcactttgtt aggacccgtt caccacgcag gacccgaaga gcacggagtt cgaaatttgg
     4561 gcatccgaac ccttcgcacc ttcgtgtcct tcgtgatcaa atatattcca catttggctg
     4621 cagccattac gctcggctaa ttgtgcccgt tacttcgcgg gatacaagcc gttgaaaaaa
     4681 ccgctttgct cgagaccgcg cacgagggag ccgtcgatct gctcgtcgac cgacagcggc
     4741 cgcagcgccg gttcgctttt ctccaggaag cttttgatca gcgcgatgcc cgcccggttc
     4801 ggatacggca cgcgtgggac atagttgaga taggttcttt gggtttcttc ctgtactaga
     4861 atgtcgtcga tcttcaacct gtccttaatg atcttcttgg aataggccgg atcattcttg
     4921 aaaaccgcga tcgcctcgct gacggcccgg acaaagttac gggatgtctc tggattttgc
     4981 cgcaggaata acttgcgtgt caccacaccg gcggcttgat agtctggaat gacatcggca
     5041 agatcgacca actcgcgaaa gccttcctgc ttggccaccg ccaacgtcgg tagagaaatt
     5101 ggcccgccgt agatcttctt gctttgcatg ccggaaagaa tcggtggcac cccgcccatc
     5161 tgaatgatcg gcacatcgac ttccggccta agcccccatt tatcgagcgc aaggcgcagc
     5221 agcgcgtcgg tggtggaacc gaagcgggtt acacccatcg ccttgccctt gagatcctta
     5281 ggctcgcgaa tctccggccg cacgatgatt gaaaacggcg gcttggtcac cagaccgacg
     5341 atcatcaccg catcgctccc ctgcgcattg acgcgcatca ccggctcgat gccctgaatg
     5401 cccagctgcg tggtgccgcc gagcagcgat tgcatcgccg gcgaaccggc gacataaatc
     5461 aattcgacgt cgagatcata ttttgcaaaa atccctttgt cgaccgcgat ccaagggatt
     5521 accgctgagc cggatatcga gctataggcg acacggagct gggtcggcgc cttgctcgcg
     5581 gcaagcgacg gcgctgaaca aaaaaccgcg atcagtatgc tgacaaatct aaacaatgac
     5641 atgaggattc ggctttcaat tgtccaatat ctgactaacc ttccgcatgc agtatcttcc
     5701 cgcctacgtc tactggcttg gcgtcgatca gaatgaaagc aatcacacaa ggctcgttgc
     5761 cgcggttgac ccagttgtgg atcgtgccgc gctgcaccag cacatcaccc gcctgcaaat
     5821 gaaccacagt gtcatcgagc tccatgtcga tctctcccga catgacaacc gcataatcga
     5881 tggaatccgt ccgatggttg cgcggcgcga cgcctggcgc aaactcgaca acgcgaaaga
     5941 ccgtgccgtt atgcaacgtc gtgccgacct gccgcagtcc ctcgtcggcg ccgccggtgt
     6001 tgttgacggg aaaagattcc gtggtccaga ccacacaagc cgaccgtccc ggccggccgg
     6061 cggcgagatt tttacaaacc tcatcgatct tcacaatcgc gcgcccgttg ccatcatgtc
     6121 cggtgactac ccgtcgtaat ttaagcgaca ttgtcgttct tcctcctcga acccggcgaa
     6181 tataagcttt gcgcgcgatc cagatctgct actaattccc tgccctgagt cggacacaga
     6241 gtttccatga gaccgcatga tgctcgcctg gggctgtaaa gctaatcggc cggcgtggaa
     6301 ttttctgcgt tctttgcggc cagttttctc tggttcgaag cattgaacat tgaacccttg
     6361 aacctttgaa cggcgcgccc aaagcacgct atttatgaaa ctgcttggtc ccggcatagg
     6421 cccgcgggtt gaccagtgcg tgctcttcct gcatcagcca ggcgatctcc gccgccatcg
     6481 gcacgctcgc cgcttccatc gacgcatgcg ccgccatctt gcagtatttc aacgtcgtct
     6541 tgtcgaattt gccgatctct tcggcccact gccatgcttc gtcttgcaac tgcgcgtgcg
     6601 gcacgacccg gttgatcaac ccggcattca ttgatttcac cgcgtcccaa tttttcccgg
     6661 tcagaatcaa ctcaaaaccg agtttggtcg gaatcagcgt cttgaacatg gtcgcgatga
     6721 tcgggtacgg caaaaagccg cgcatgatct ccggcaaacc gaacatcgct ttatccgaag
     6781 cgatcgccag atcgtgcggc aacagcaacg tgatgccgcc gcctaggcag aatccgttca
     6841 ccaccgcgat ggtcaccttg ggaaaagtcc gaatcacctc ggccacatga taggcgcgcg
     6901 gttccttgcg ccggtctttg ccgcgattct gcccgccttc agtcggaaac tccgacaaat
     6961 cccgcccggc gcagtacgcg acgtcgcccg cgccggtggt caacaccacg gcgatctcat
     7021 cgtcattgcg caactcctcg aacaccgcga gtatttcccg gatggtatcg cggctcagcg
     7081 cattgcgctt ctccggccgg ttgatcgtca acttcgccac cgcgccgcgg gtttccaaaa
     7141 tcgtaaactt gccttctttg atcattgcca tttccttcct caggtcgtcg tattataggc
     7201 gaataacact tgccatccgt ttcggtcaac catacagcca tgaagagcac gaagtcgtga
     7261 gtttttcgct atccgaactc ttcgtactac gactggaaat aatttgcgcg acttgggcaa
     7321 atttccgcat gcgccaaagt caagaggtcg ggtgtcaaac ccgccccacg tttgactctc
     7381 tttttgcccc cttacgttct ttgcggccat tcttcttggg gtttggcttt gtggtgcatg
     7441 tggaattttt cttcgactac gacatggcga tattgccttg aaagccgtca gccgatcgga
     7501 tatctccgtg taaaatggaa tccaccatcc gccggatcag taaatccgta ttcgccatca
     7561 tcggccctga aggggctact aatttcacca tcatcaaggg cgccaacggc agcgcggtgc
     7621 tgattgacgc cgatattcgc cgcatcgacg aagtcgaaga agcgttgcgg ctcaccggtt
     7681 gcgccggcgt cagctatttg atcgacaccc atgagcactt cgaccatacc tcggccaatt
     7741 tttacttcgc gcaaaagcag atccccatcg tcgccagcgg cggctgcgtg caagcgatga
     7801 aagacgacgg tgccgcggac ttcgcgcgca tgctaaagcc cgtaccggag ctataccaac
     7861 gatttcccgg cctgtgtcta accttgcccg atgtcgtgtt tagctcgaca acccaaataa
     7921 aagtgccggg tgtcacattg gatctggttt accgggccga gaacggccaa agtcatagca
     7981 agggcgatac gacaatttat tttcacgaag aagaagtttt catcgccggc gatctgctct
     8041 ataccgaagt ccatccggtg acgttcttcg gcaacattcc caactggctg acagccttaa
     8101 aaccgctcat ggattaccgc tacaatcaat tgattcccgg tcatggacca gccgtcgagg
     8161 gcgagcagtc cggcagagcc tacttcaagc gcatgcacga ttatctcgaa gacttttacg
     8221 gccgtctagt ggagatcgat tcgggccgca caagccggga agaagtcgcg cgtcatatgc
     8281 aaagcggcgc ttacgccgag cttggcaaaa cgcgcatggt ggaacgcaat atcaaccagt
     8341 ttcttaccgg aaggtggttt tagatgaagc tgacagccgt ccttctcatc gcccttgcgc
     8401 tgatagctgc cggcaacagc ctagaagccg ccgaaaagct ccgcgccgcc tacccggcca
     8461 tcgcgcccgg ctcgactccc tcttgggtta cggcggaaag aggcatctgg cgcaaatatg
     8521 gcttcgacgt tgagccgatt ctggtgagtg gcggtgcgcg cgccgtaccg gcactgatcg
     8581 gcaatagcgt gcaatttctc attggctcgg ataccggcgt gaccacggcg cagttgcaag
     8641 gcatcgccat tgtccggctc ggcgtgacca tgaacactct cggctcgtcg ctattgaccc
     8701 agcccagcat tcaaacggtt caagacttaa agggcaaaac tctgggcatc tcgcgcgggc
     8761 gcgatgcgag ttatattcgg ttggccaaat tactgcgcga taatggcctc gaccccaacc
     8821 aggacgtgaa atttctttcc atcggcggcg gtgaaggcgg ccgagtctcg gcgctgaaag
     8881 ccggcgtcat ccatggcacc atgctgttcc cgcccttgga cttgatcgcc agaaatgatg
     8941 ggttaaaagt cctcagcaaa ttcgacgtgc cgacgccggg cggcggcatc agcacgagcg
     9001 cagcgctgct caagcagaac cgcccgttgg tgctgaactt cctcaaaggc tacatcgaag
     9061 ggatccacta tatgatcggc cacaagccgg agagtctgaa aattctccag cgctactttc
     9121 aaaactccga ccttacggcg atgggctatc tctacgacga gacgacccgc cgtttggaaa
     9181 aagaactacg cgccagtccg gagtccatcg ccttccatta cgacatggcc gcccaagacg
     9241 acccacgcgc caagcaagcc accgagaaaa cattttggga taatagtctg atcgaagaga
     9301 ttcgccgctc gggatttatc gatcagctgt ataaaaatta aagctcgtaa aagtaagcat
     9361 gagtggcgaa tcgaccatcg tgcctctcgc tacttcctat agagttgctc caaaaacccc
     9421 gagcgctgaa tctcgtcgat caaactgaga tcccaattat ccttctcggt cagcttcttc
     9481 gcctggggaa aatcgagagc gacgagatcg agcaacgcgc gcaccgcatc cgattgcgga
     9541 cgcaaatctt tttccgcgcg ggtggaaatt tcttcgtaga gataggccat gatttcgaga
     9601 tcggggttgc gcacatattt ggcaaaaatc ttcaaactct cgcttctgtt cgccttcaag
     9661 tagtgaatcc cttccatata gccgcgcagg aaccgcaacg cctgcggccg gttcttttgc
     9721 acgaatgcag gagtcgtatt gatgccgccg ccaacgttgg cgacatcgag ccgggagaga
     9781 atgctcatgc ccgacttctc ggagatctta tccgatggcg gcgtgaacat ggtcgcgtga
     9841 atcacaccgc tggacagcgc cgccacgcgc gccgccgggc cgccatctcc caccgataga
     9901 aacgtgacat ctttcgccgc gtccatgccg ttgtcgcgca gcagtttaac gacccgcgca
     9961 taagcggcgt cgcgcccggg cgtgatgccg atgatcttgc ccttgagatc gcggacggtt
    10021 ttgatcccgg gctgaacgat catggaatac cccaaggtgt tggtggtcac gcccagccgc
    10081 gtcaaactcg ccccctggat aatccctgtt gtgattccca ggtccgagcc aagaatgaaa
    10141 tcgacactgc cgccgatcaa cgcggcgacc gttctgccgc cgccgcgcag aaagatcagc
    10201 tcgacatcga ggccgtgctt tttccaaaag cccttgtccg cggtgaccca cgacgcggca
    10261 aatccggttg cgagagaggg atagcccacg cgcaagacat ccgcgctcca caacttcgat
    10321 gtcggcacta ggaagcacgc gaccagcgca aatccgattg acagtgattt cattgaaact
    10381 ccattcgcca aaacatattc tcgcccatga tccgccgagc ctcagtagtc gaacgacttg
    10441 actcttcaat ggctcgaacg atttgacgcc gcggctacaa cggtctgccc tagctaacac
    10501 ccacgccgag acctgtcaaa taaacaaagc ctttcgcgta gcgtctacac ccgcacgcga
    10561 cgaaagggga tgctcctctt ttggattccc cattgatagt gcccggagca agtgcggcga
    10621 gtgtttagat tcgaaaaccc ggcgcgcgaa ttgacatcgg cccggcacaa cgcgtaattt
    10681 caacgttcca aactacagcc cgacggtggc gaaggtgaaa taaaataaca caagtttaaa
    10741 ccaaagagct cggaattgga atcttatact ctatattccc tcctaacccg gatgaacgat
    10801 caatgaacat cggcgaatcc ttaacccgca acgcccaaca ctttcccgac aagcgcgcca
    10861 tcgtcgatgc gcaccgggcg gtaacctttg gcgaattgca cgagcgcacg gatcgattgg
    10921 ccaactatat gctcgatcta ggtgtcggca aaggtgatct ggtcggtctt tcggtgggca
    10981 gccggaccga gcacttcgaa gcgctcttcg ccattgccaa gatcggcgcc atggcgattc
    11041 ccttcgattt caattggagc gcgcaggaat gcgagtcgat gttgcgcttc ttggcaccca
    11101 aggcgttgtt cgtcgaaata cgcaaagaga ccgagcccct gctcgagctg gcgcgcgagt
    11161 tcattcctgc cgaaaagctg atcctgattg cggctgacaa ctgccagggc ggagtcggca
    11221 tggaaagcat tatcgagtgc ggcaatccca gcgagccaac aaccttcgtc gagggtaagg
    11281 atccgttcct gctaatgatc acctccggca ccaccggttt ccccaaggcg tgtagcatcg
    11341 atcaccagac ctatgcgctg cgctgtctca attacagtgt cagcaaaggc atgaaccaca
    11401 acgaacgcgc tttgatggcg ctgccggtcc acttcaacgc cggcagaggc tcagtgatgg
    11461 cgattctcta tctcggcgga acc
//
LOCUS       DASQKS010000315         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3531184, whole genome shotgun sequence.
ACCESSION   DASQKS010000315 DASQKS010000000
VERSION     DASQKS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531184"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VE170_08220"
     CDS             complement(<1..1011)
                     /locus_tag="VE170_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ15460.1"
                     /translation="MALFLANEDVQQLLTMGDYIDAVEKAYHELGMGRAWNNPRIHTY
                     GQAQSGATHFLKVFTGTVPALGYSILRIDSTMERNESGHETSRKIGNRNMGWLLLFSV
                     ESGQLEAIIEDRALQRMRVGAMTGIAAKYLAKSDSQTVGIFGSGTQAGPQLEALCKVM
                     KIRRIKVYSPTVKNRENFAAKMTDALGAEVTAVDSPRQAVTGCDIVVVATNSNEPVFD
                     GRWLEAGSYVSSIVNSDKVLRRRDLDDESFRRAALIVVSTRDQIKNDEPIWLTEGLEC
                     GAVSWDRVWEIGELVCGKAPRRSDDRQITILKNNGLSVQFAGVGAAVMKRAKERGLGK
                     ELP"
     gene            complement(1064..2170)
                     /locus_tag="VE170_08225"
     CDS             complement(1064..2170)
                     /locus_tag="VE170_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ15461.1"
                     /translation="MTLIINNKEVEQVLTMEDTLAALEQSYLQLAAQEAVCRPRIDIR
                     IPTSDPARNYQWGTMEGGSTAGYFAIRMKSDIVYEKKYNGVVTQEKYCMRPGLYCGLI
                     LLTSVENGEPLAFINDGYLQHMRVGADGGIGVKYLANQDAEVVGMLGAGGMARTHMEA
                     FTRVRKIDKLQVFSPTRESRERFGREMAEKYNIEVKVCSRPEEVYKGAHILAALTDSA
                     VEVTDGSLLEKGTHIVVVGGTGKPDAESLKKVDVYLRFGDTPAPIGHPELATDAEHVG
                     YEARPQQNKYGDGRRDRRKHGNTLPDRRVTLADLVNGRVKGRSSADQITYSERGNLQG
                     AQFHAVAGKVYELAKGAGLGREIPTEWFLQDIRD"
     gene            complement(2309..2728)
                     /locus_tag="VE170_08230"
     CDS             complement(2309..2728)
                     /locus_tag="VE170_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ15462.1"
                     /translation="MATQFTGGCLCGRVRYECSAEPFFMGNCHCRDCQKSSGGAYEPD
                     IGLPAAALKITGTVKYYDGKADSGNILSRGFCPECGASLFGKSSAMPDLAMITAGSLD
                     DPSLYKPTLDIFTSSAQPWDHMDPALAKFPKMPPIGG"
BASE COUNT          529 a          870 c          840 g          693 t
ORIGIN      
        1 cggcagctct ttgccgagcc cgcgttcttt ggctcgtttc atcaccgcgg cgccgacacc
       61 ggcgaattga acgctgagtc cgttgttttt caggattgtg atttgtcggt cgtcgctgcg
      121 ccgcggtgct ttgccgcaaa ccagttcgcc gatttcccaa actctgtccc aactcaccgc
      181 gccgcattcc aagccttcgg tcagccagat cggttcgtcg tttttgatct ggtctcgcgt
      241 gctgacgacg atcagcgcgg cgcggcggaa actttcgtcg tcgaggtcgc ggcggcgcag
      301 caccttatcg ctgttgacga tggagcttac atagctgcct gcttccaacc agcggccgtc
      361 gaagaccggt tcattcgagt tggtcgcgac aacgacaatg tcgcagccgg tgaccgcttg
      421 ccgcggtgaa tctaccgcag tgacctcggc gccaagcgca tcggtcatct tcgcggcgaa
      481 gttttcacgg ttctttaccg tcgggctgta gactttgatg cgccggattt tcatgacctt
      541 gcagagcgct tccagttgcg gcccggcctg ggtgccggag ccaaaaattc cgacggtttg
      601 gctgtcgctt ttggcaagat acttggcggc gatgccggtc atcgcgccaa cgcgcatgcg
      661 ttgcagcgcg cgatcttcga taatcgcttc gagttggccg ctctccaccg aaaatagcag
      721 cagccagccc atgttgcggt tgccgatttt ccggctggtt tcgtggccgc tttcgttgcg
      781 ctccatggtc gagtcgatgc gcagaatcga ataacccaga gccggtacgg tgccggtgaa
      841 aaccttgaga aaatgggttg cgccgctttg ggcctgtccg taggtatgga tgcgcggatt
      901 gttccacgcc cgtcccatgc cgagttcgtg ataggctttc tccaccgcgt cgatgtaatc
      961 gcccatggtt aagagctgct ggacgtcttc gtttgcgaga aacagcgcca tcgtcgattt
     1021 cccttcgcgt cgagttgaac gaccggaact tttgaaagag ctgtcaatcc cgaatgtcct
     1081 ggagaaacca ttcggtcgga atctcgcggc cgagaccggc tcccttggcg agttcgtaga
     1141 ccttgcccgc caccgcgtga aactgggcgc cctgcagatt gccgcgctcg gagtaggtaa
     1201 tctgatccgc cgagctgcgc cctttcaccc ggccgttgac caggtcggca agcgtcacgc
     1261 gccgatccgg cagggtgttg ccgtgcttgc gccgatcgcg gcggccatcg ccatatttat
     1321 tttgctgcgg gcgcgcctca tagccgacat gctcggcgtc ggtggcgagc tcgggatggc
     1381 cgatgggcgc cggggtgtcg ccgaagcgca ggtaaacgtc gacctttttc aggctctcgg
     1441 cgtccggttt gccggtgccg ccgaccacga cgatgtgggt gcctttctcg agcagtgagc
     1501 cgtcggtcac ttcgaccgcg gagtcggtga gcgcggccag gatgtgcgcg cctttgtaga
     1561 cctcttccgg gcggctgcag acttttacct cgatattata tttctccgcc atctcgcggc
     1621 cgaagcgctc gcggctttcg cgggttgggc tgaacacctg cagcttgtcg attttgcgca
     1681 cccgcgtgaa cgcctccata tgcgtgcgcg ccatgccgcc ggcgcccagc atgccgacca
     1741 cctccgcgtc ttggttggca aggtatttca caccgatgcc gccgtcggcg ccgacccgca
     1801 tatgctgcag gtagccgtcg ttgatgaacg ccagcggttc gccgttctcg accgaagtca
     1861 gcaggattag cccgcagtaa aggccgggcc gcatgcagta tttttcctgg gtgacgacac
     1921 cgttgtattt tttttcataa acaatgtcgg acttcatgcg gatcgcaaag tagccggcgg
     1981 tcgatcctcc ctccatggtg ccccactggt agtttcgcgc cggatcgctg gtcggaatcc
     2041 ggatgtcgat gcgcggccgg cagaccgctt cctgggcggc gagctgcaaa taagattgtt
     2101 ccagcgccgc aagggtgtcc tccatggtga ggacctgttc gacttctttg ttattgatga
     2161 tcagtgtcat tgtcgtcctt acctctcatc cttcctactc ttagcgcctc acacgtctta
     2221 aattcgcgct aatcaaagtt ccgagtttcg ctccgttaaa cgatttgaac ggagcaaagc
     2281 ggttgaacca cttgaacggt ttgaactcct atcctccgat gggcggcatc ttgggaaatt
     2341 tggccagcgc cggatccatg tgatcccaag gttgcgcgct gcttgtgaag atatccaagg
     2401 tcggcttata caggctcggg tcatcgaggc tccccgccgt gatcatcgcg agatccggca
     2461 tggcgctcga tttgccgaac aaactcgcgc cgcattccgg gcaaaaaccg cggctcaaga
     2521 tattgccgct gtccgctttc ccgtcgtaat acttaaccgt gccggtgatc tttagcgccg
     2581 cggccggcag tccgatgtcg ggttcatagg caccgccact cgacttttgg caatcgcggc
     2641 agtggcaatt gcccatgaaa aacggctcgg cggaacattc gtagcgcacc cggccgcaaa
     2701 ggcagcctcc ggtaaattga gtagccatag ttgttactcc ttctttgtcc gtgaattctt
     2761 aacatcacta ctatcgccaa tagttagaga aatcgatatt tgcacgactc gacgatagat
     2821 atgctcgtga ttgagacgat ttgactagca gcttgctgaa aaaaggttag gaatgcttcg
     2881 agagcctcag catgaacaga aaaacatcaa cgatatcaaa tcgcctccgt ta
//
LOCUS       DASQKS010000316         9772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2272135, whole genome shotgun sequence.
ACCESSION   DASQKS010000316 DASQKS010000000
VERSION     DASQKS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272135"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VE170_08235"
     CDS             <1..438
                     /locus_tag="VE170_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ15463.1"
                     /translation="NRRIVGIDGDGALLMNACGMPTLGWQNPANLTLLVFDNGIYEAS
                     GLRKTATSAGADLMAMAAGAGVKHASWAASLDEFRQLLRQALGRKALSMIGVKSEAGE
                     EYFKSWHQLPAFEYGEVENLYRFMRHIEKLEGRRIVPLPGPRH"
     gene            510..1523
                     /locus_tag="VE170_08240"
     CDS             510..1523
                     /locus_tag="VE170_08240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYJ15464.1"
                     /translation="MKFDVSLLVHDLSQMPALARFADELGFDGIWTFETSHEPYLPLV
                     LAAEHSRRLNIGTSIAVAFPRSPTITAQIAWDLARFSQGRFILGLGTQVKGHIERRFG
                     VKWEQPVAKMRDYILAVRALWDSWLHQTRLNYQGEFYKLTLMTPFFSPAPHQYSRIPI
                     FVAGVNRRMCELAGELCDGFHVHPLHTERYLREKILPNIESGLAKSGRPRSALELSSS
                     IFVIPSDEPEQAARYEADVRQQIAFYASTPTYRPVFDLEGWGQTADELKALAARGHWN
                     EMPSLINDEMLERFAVRGTWVELPMLLRQKYSGLLDRVSYYFPLVPGENEAGWRATAA
                     GLT"
     gene            complement(1654..2655)
                     /locus_tag="VE170_08245"
     CDS             complement(1654..2655)
                     /locus_tag="VE170_08245"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15465.1"
                     /translation="MRRFLACVTGLLAVIATVSLAPAQTSLNNVILGHSGGAGSLGNL
                     RRIIERDKLWEKHGLNVKSVYFSSGGILTQAMAGGNIAGSESEVPGMLNLAVSGVLDL
                     KLVTVTINKIEHVFIVRKTITKPEELKGKRLAISRIGSASDTVTRLVLKSWKIDPDKE
                     VTVLQSGNTPTRMTALAAGHVDGALVSPESIHKILASNCCRILADLSELPMDYARYGY
                     VFPTAWLKTNRDVVRRLLMGYLEGIAIFRTRPRAAFAALEDEGIKDPAVQKDIYERAL
                     KHVREYPVPEPNGIQNVLDSLTHPNARTVKPAAVMDTSIMEEIKKSGFIDKLYGRGS"
     gene            complement(2937..4379)
                     /locus_tag="VE170_08250"
     CDS             complement(2937..4379)
                     /locus_tag="VE170_08250"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15466.1"
                     /translation="MTPANHDLRAFLDEVEKIGELRRIENIPWDKDIGGLVEMILERS
                     ANPPAMLFEKIPGARQDMEILCSQIDTLPRLAIAMGTDPNLGLTDFIQAWRRKIRNFE
                     PMPAQFVKDAAIFDNCADHNIDLEAFPIPRWHEEDGGRYIGTDDLVITEDPEEHWINA
                     GTYRVMLQGKDAVALYISPGKHGRVQRQKYFDAGKPCPVVMSFGHHPILFLAGCTDVP
                     NKMSEFDYAGGVIGEPIQLARGPLTGLPIPAYSEFAIEGEMVPGDDLPEGPFGEWPGY
                     YASATRPEPVVRVKALYYRNNPILCGEPPLRPSAGQGFHRSIQRSALIWNALEDAGVP
                     DVHGVWLHPAAYRFFSILKIKQRYPGHAKQAALIASQCRPGAYLGRYVVVVDEDVDIY
                     NPNDVIWAIGTRSEPENIDILRRCWSGPLDPAIPRERKGFNSRAIIDATRPYEWKDKF
                     PAVNAVSERLKAELDKKYPALLKDMIGTRH"
     gene            complement(4453..5226)
                     /locus_tag="VE170_08255"
     CDS             complement(4453..5226)
                     /locus_tag="VE170_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ15467.1"
                     /translation="MAMSAPKLGVKMSPDEAKAYIAEVNERVVQRWTERVTNGPFMSS
                     FLDGNLPMPAVKLFFKNWGNFTVEINTLVAVSYHKHIGFFKRHFDLMAPLGAKIADEF
                     IHPKPPGHVMVMLQTAAAFGLTQDEILREPMLPECRAILDFKRQLMWEGTVAEWWFSM
                     LTEEPIGHWSAMWFRTLTQKYGFSKPQAVYFSTHEEADLKEHEDGVMGHGSFNKLVMQ
                     RILEDGYADCREGYNLEYCATAAVDLYGVMHRAAVEHAA"
     gene            complement(5358..5828)
                     /locus_tag="VE170_08260"
     CDS             complement(5358..5828)
                     /locus_tag="VE170_08260"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ15468.1"
                     /translation="MNSRMKTEGLLFTLFVGVVMASALWFARDWPVRASIGVLFLGGI
                     GVVLALWQFILDAKRSDANQQRSQFEMPTMAADSQWGNVEIWSWIIGFYLLILLVGFP
                     IAVPLFVLSYSKFYGANWRLGISLSVIAWGFVYGVFEKILHVPWPEPLLFSLYR"
     gene            complement(5825..7312)
                     /locus_tag="VE170_08265"
     CDS             complement(5825..7312)
                     /locus_tag="VE170_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ15469.1"
                     /translation="MIEAALEGLYQVLTPNSLALMCLGVLLGSLVGFLPGIGGPSTLA
                     IMLPFVMTMKDPYAVIALLVGFDAVGNTASAFTAVLISVPGGSGSQATVLDGYPLAKQ
                     GQAMRALSASFFASLVGGLIGPIVLIASLPFLRPMVLSFGSPEFFMLTFWGVTMVGVL
                     SGNVPLKGLLAGILGILIAAVGADDKSGIERFTFDTAYLIEGIDLVLVALGVFAIPEC
                     IDLAARRTKVAETQELGEGFWQGARDVFKHWWLTLRASAIGIWVGFLPGLGSSVADWF
                     AYAHAVQTEKHPENFGKGDIRGVIAAESSNNSKEAGDYIPTLAFGIPGGTSTALILTA
                     LIAVGINPGPEMLTTQLNLTFAVVWTLTIANIVAVAICLAFIKPISRMCFMPFYSLVP
                     MILVFVFIGAFAANRNNLDLIALLGFSFLGFIMRRHGWPRSPLILGLVLGDKMENYLW
                     LSYGRFGFEWLTRPGVMIIAALVVLSLCYPMLQHWRANKDRTGQP"
     gene            complement(7316..8386)
                     /locus_tag="VE170_08270"
     CDS             complement(7316..8386)
                     /locus_tag="VE170_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15470.1"
                     /translation="MQSKKIVHLFLLVGQIGFAASTSTVIAANAPVTFQGKTLTILIS
                     SSPGGGTDTAGRLISQFLPKYLPGNPVTVVQNMPGGGGTIANNHFYANAKPDGLTLLQ
                     DSSSAIGNMNRGGSRIKYDHRKNRAVGSIARGGSILMVRKELKDSLTDVKSRKLVVGD
                     TDGIRTWVSMTVWSAEYLNWNLRWLYGYKGSRELSLALRQGEIDLWATSNSRLISDMR
                     KEGFIEMIYQQDEERRADFRDVPTFKEYLGNKKPSGVSWDAYQAWANSEDLDKFIVAP
                     PKTPDNVVAALRDGYMKMNKDDEFKSLATKFFGESWSLSSGEQTEALIKKLTSIPKPA
                     QDFLTQIRKKYGLPAGGAASES"
     gene            complement(8508..9419)
                     /locus_tag="VE170_08275"
     CDS             complement(8508..9419)
                     /locus_tag="VE170_08275"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15471.1"
                     /translation="MDKVNFPYRSDSHLPFLHVVHESGSWAKHGLDVNYDYEITSADS
                     HSNVANGSVEFVGGNHLSPYIRRPTGDKWVYLGQTVSLLNHRLVVRADSGINKLADLK
                     GKHIATKGNHPGLNTWLFIKQAGLLNDCRVEKTKGGKFHWEAVRDGEADAAFVTPPAD
                     ILSKRAGLKVIDVSFLPMVWFTTVSSSSMFVEKHPDIVDKMLRGVCEGIAYFKTHRAE
                     CIKIIKDKYKAEGELDDETASHLYDDLKLILQAKPYATLPAISNVYEIAIEQNKAAEK
                     CDPMALWDFHHLRRIDDSGFIDNLYRT"
     gene            complement(9579..>9772)
                     /locus_tag="VE170_08280"
     CDS             complement(9579..>9772)
                     /locus_tag="VE170_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYJ15472.1"
                     /translation="ETCLGSGKYQAAIKQDIEDGTRAGVSGTPAFFINGRLVSGAQSL
                     ETFARVIDDELARGAAGKK"
BASE COUNT         2005 a         2696 c         2993 g         2078 t
ORIGIN      
        1 aaccggcgca ttgtcggcat cgacggcgac ggcgctctgt tgatgaatgc ttgcggcatg
       61 ccgacgctcg gctggcagaa tccggcgaat ctgaccttgc tggttttcga caacggcatc
      121 tacgaagctt cgggattgcg aaagacggcg accagcgcgg gcgccgatct gatggcgatg
      181 gcggctggcg ccggtgtcaa gcacgcgtcc tgggccgcgt cgctggacga gttccgccaa
      241 ctgttgcgcc aagctctggg gcggaaggcg ttgtcgatga tcggcgttaa gagtgaagcc
      301 ggcgaagagt attttaagag ctggcatcag ctgcccgctt tcgaatacgg cgaggtggaa
      361 aatctctatc gcttcatgcg tcacatcgag aaactcgaag ggcgccgcat cgtgccgctg
      421 ccggggccga ggcactaagc aaaaagatac cgcctggaga ttttccagct tggtacgggg
      481 tcagcgaccg cactgaatga attcctagga tgaaatttga cgttagtttg ttggttcatg
      541 atctcagcca aatgccggca ctggcgcgct ttgccgacga gttgggattc gatggtatct
      601 ggaccttcga gacttcccat gagccgtatt taccgctggt gctcgcggcg gaacatagcc
      661 ggcggttgaa catcggcacg agtatcgcgg tggcgttccc gcgcagtccg acgattacgg
      721 cgcagatcgc ttgggatctg gcgcgatttt cccagggccg atttattctc ggtctcggca
      781 cccaggtcaa aggccatatc gagcggcgct tcggcgttaa gtgggaacag ccggtggcga
      841 aaatgcgcga ctacattctc gccgtgcgcg cgctttggga cagttggctg catcagaccc
      901 ggctcaatta tcagggagaa ttctacaagc tgacgttgat gacgccgttc ttcagtccgg
      961 cgccccatca gtattcgcgc attccgatct tcgtcgccgg ggtcaatcgg cgtatgtgcg
     1021 aactcgccgg tgaattatgc gacggctttc atgtccatcc gctgcatacc gaacgttacc
     1081 tgcgcgagaa gattttgccg aacatcgaat ccggattggc gaagtcgggg cgcccgcgat
     1141 ctgcgctcga attgtcgagc agcattttcg tcattcccag cgacgagccg gagcaggcgg
     1201 ccaggtatga agccgacgtg cgccagcaga ttgcgttcta cgccagcacg ccaacctacc
     1261 ggccggtgtt cgatttggaa ggctggggcc agactgccga tgaactcaaa gcactcgccg
     1321 cacgcggcca ttggaacgag atgccatcct tgattaacga cgagatgctc gaacgcttcg
     1381 cggtgcgcgg tacttgggtg gaactgccaa tgctgctgcg gcaaaaatat tcggggctgc
     1441 tcgaccgcgt gagttactac tttccgctgg tgccgggcga gaacgaagcc ggatggcgcg
     1501 cgactgctgc tggcctcacc tgatagtatc cataaaagcc gactttccac ttcgtcgctc
     1561 agcgcgatgt ccattaattg caccgctttt gccttgcgca ttcgcagcgc ttaacgtcag
     1621 cagattcaaa atcgtccgcg gtagtttatg acattagctg ccgcggccgt agagtttgtc
     1681 gatgaagccg gatttcttga tctcttccat gatgctggtg tccatgaccg ccgccggttt
     1741 gacggtgcgc gcgttgggat gagttaacga gtcgaggacg ttttgaatgc cgttcggctc
     1801 gggcacgggg tattcgcgca catgcttgag cgcgcgttcg taaatgtctt tttgtacggc
     1861 cgggtctttg atgccctcgt cttcgagggc cgcgaaggcg gctcggggcc gcgtgcgaaa
     1921 aatcgcgatg ccttcgagat aacccatcaa gagtcggcgc accacgtcac ggttggtctt
     1981 gagccatgcg gtgggaaaca cgtagccgta gcgcgcgtag tccatcggca actcggaaag
     2041 gtcggccaat atgcggcagc aattggacgc caggattttg tgaatgctct cggggctaac
     2101 caaggcgccg tcgacatggc cggcggcgag cgcggtcatg cgcgtcggcg tgttgccgga
     2161 ttgcaacacc gtcacttcct tgtccgggtc gatcttccat gacttcagta ccaggcgcgt
     2221 cacggtatct gaggcggagc caatgcggct gatggcgagc cgcttgcctt tgagttcttc
     2281 cggcttggtg atggtcttgc gcacgataaa cacatgctcg atcttgttga tggtcactgt
     2341 gacgagcttt aagtcgagca cccccgacac cgccagattc aacatgcccg gcacttccga
     2401 ttccgatcca gcgatgttgc cgccggccat ggcctgagtc agaatcccgc cgctgctgaa
     2461 gtaaacgctc ttgacgttca aaccatgttt ctcccagagc ttgtcgcgct cgataatccg
     2521 gcgcagattg ccgagggagc cggcgccgcc gctgtggccg aggatgacgt tgttgagcga
     2581 ggtttgtgcc ggggcgaggg acaccgtggc aatgaccgcc aataatccgg tgacgcatgc
     2641 aagaaatcgt ctcattgtgc ctccctgacg aattcaatgt cgcagccgca tggctacgat
     2701 cgacaaaacc atcgcgtcat attcaatgac ccgttccgtc atgccggcga aggccggcat
     2761 ccaggtttgc tttcggcgcc agactgagaa ccgcctggat tcccgttgcc acgggaatga
     2821 cggaagagga cccggagccg aatggatttt tcgctgcgct cgaaatgacg gtctgctgtt
     2881 tttgcgcctg tcatcccgaa cggagtgatg gatctcgtct tattccgcac ggaacgtcag
     2941 tggcgcgtgc cgatcatgtc cttcaacagc gccgggtatt tcttgtcgag ttcggctttc
     3001 aatctttcgc tcacggcgtt taccgcgggg aatttgtctt tccattcgta gggccgggtg
     3061 gcgtcgatga ttgcgcgtga gttgaaacct ttgcgttcgc gcggaatcgc cgggtccagc
     3121 gggccgctcc agcagcggcg caggatgtcg atgttctccg gctcggagcg cgtgccgatg
     3181 gcccagatga cgtcgttggg attgtagatg tcgacgtctt cgtcgacgac gacgacgtag
     3241 cggccgagat aggcgccggg ccggcattgg ctggcgatca gcgccgcttg tttggcgtgg
     3301 ccgggatagc gctgcttgat ttttagaatc gagaaaaatc gataggcggc gggatggagc
     3361 cacacaccgt gcacgtccgg cacgccggcg tcttcgaggg cgttccaaat gagtgccgag
     3421 cgctgaatcg agcgatgaaa gccctggccc gccgacggcc gcagcggcgg ctcgccgcag
     3481 aggatcggat tatttctgta atacagcgct ttgacgcgca ccaccggctc cggccgggtg
     3541 gcgctggcgt agtagcctgg ccactcgccg aacgggcctt cggggagatc gtcgccgggg
     3601 accatttcgc cctcgatggc gaactccgaa taggcgggga ttggcagtcc ggtgaggggc
     3661 ccgcgcgcca gttgaatcgg ctcgccgatg acgccgccgg catagtcgaa ctcgctcatc
     3721 ttgttcggca cgtcggtgca gccggcaaga aacaagatcg gatgatggcc gaaggacatc
     3781 accacggggc agggcttgcc ggcgtcgaaa tatttttgcc gctgcacgcg gccgtgtttt
     3841 cccggcgaga tgtaaagcgc aacggcgtct ttgccttgca gcatgacgcg gtaagtgccg
     3901 gcgttgatcc agtgctcttc ggggtcctcg gtgatgacca gatcgtcggt gccgatatag
     3961 cggccgccgt cttcctcatg ccagcgcgga atcggaaacg cttcgaggtc gatgttgtgg
     4021 tcggcgcagt tgtcgaatat cgccgcgtct ttgacgaact gtgccggcat cggttcgaag
     4081 ttgcgaatct tacggcgcca ggcttgaata aaatcggtga gcccgagatt cgggtcggtg
     4141 cccatggcga tggccaaccg cggcaaggtg tcgatctggc tgcagaggat ttccatgtcc
     4201 tggcgcgcgc cgggaatctt ctcgaagagc atggccgggg gattggcgct gcgctcgaga
     4261 atcatctcga ccaagccgcc gatgtccttg tcccaaggga tgttttcgat gcggcgcagc
     4321 tcgccgattt tctcaacttc gtctaagaac gcgcgcagat cgtgattagc tggggtcatt
     4381 gttcaatatc ctcgaagagg ttcaaaacgc tcaagccgtg caaaatgttc aagccgttaa
     4441 acctaaaagc tctcaggccg catgctcgac cgccgcgcgg tgcatgacgc cgtagagatc
     4501 gacggcggcg gtggcgcagt attcaagatt atagccttcg cggcagtcgg cgtagccgtc
     4561 ttccaagatg cgctgcatga ccagcttatt gaagctgcca tgtcccatga cgccgtcttc
     4621 atgttctttc aggtcggctt cttcatgggt ggaaaaatag accgcttgcg gtttcgagaa
     4681 gccgtatttc tgcgtcaagg ttctgaacca catggcggac cagtggccga tcggttcttc
     4741 ggtcaacatg gagaaccacc actcggcgac ggtgccttcc cacattagct ggcgtttgaa
     4801 atccaagatc gcccgacact cggggagcat cggttcgcgg agaatttcgt cctgggtcaa
     4861 gccaaaggct gcggcggttt gcagcatgac catcacatgg ccgggcggtt tggggtggat
     4921 gaactcgtcg gcgatcttgg cgccgagggg cgccatcaaa tcgaagtggc gcttaaagaa
     4981 gccgatatgc ttatggtacg acaccgcgac cagagtgttg atctcgacgg tgaagttgcc
     5041 ccagttcttg aaaaacaatt tgaccgccgg catgggcaaa ttgccgtcga gaaatgaact
     5101 catgaacggg ccgttggtga cccgctctgt ccagcgttgc acgactcgct cgttcacctc
     5161 ggcgatgtaa gccttggcct cgtccgggct catcttgacg ccgagtttag gcgcgctcat
     5221 agccatttaa cttcctttct aagaaattgc ttctatccca tgcgacggtt catttctagg
     5281 gcgccgatcg cagcgttcgg tcttcatcat tccaggatcg agcggtaccg ttaaacgctc
     5341 aaccggaacg gcaatggcta gcggtacagc gagaagagca gcggttcggg ccaggggaca
     5401 tggagaattt tttcaaagac gccgtagaca aagccccagg cgataacgct cagcgagatg
     5461 ccgagccgcc agttggcgcc gtaaaattta ctataactta agacgaacag cggcacggca
     5521 ataggaaagc cgacgaggag gatcagcaga taaaatccga tgatccagga ccagatttcg
     5581 acgttgcccc actgggaatc ggctgccatc gtcggcatct cgaattgcga gcgttgctga
     5641 ttggcgtccg aacgtttcgc gtccaggata aactgccaga gcgccaagac cacgccgatc
     5701 ccgccgagga acaagacgcc gatggacgcg cgcaccggcc aatcgcgcgc gaaccatagg
     5761 gccgacgcca tgaccacgcc gacaaacaac gtgaacagca ggccttcggt cttcatgcgc
     5821 gaattcatgg ttgccccgtt cgatctttgt tagcgcgcca gtgctgcaac atcggataac
     5881 acagcgagag cacgacgagg gcggcgatga tcatgacgcc gggccgggta agccattcga
     5941 agccgaagcg gccgtaggaa agccagagat agttctccat cttgtcgccc agcactaggc
     6001 ccaagatcag cggtgagcgc ggccagccat ggcggcgcat gatgaagccg agaaacgaga
     6061 agccgagtaa cgcgatcaaa tcgaggttgt tgcggttggc cgcgaaggcg ccgataaaca
     6121 cgaacaccaa gatcatcggc accaaggagt aaaacggcat gaagcacatg cgcgagatcg
     6181 gtttgataaa tgccaagcag atcgccactg cgacgatgtt ggcgatggtc aaggtccaaa
     6241 ccactgcgaa ggtaaggttg agctgggtgg tcagcatctc cgggccggga ttgataccga
     6301 cggcgataag cgcggttaga atcaaagccg tcgatgtccc gccggggata ccgaaggcga
     6361 gggttggaat gtagtcgccg gcttccttgg aattgttcga cgactccgcg gcgatgacac
     6421 cgcggatatc gcccttgccg aagttttcag ggtgtttctc ggtctgcacc gcgtgggcgt
     6481 aggcgaacca gtcggcaacg gagctgccga ggccggggag aaacccgacc cagatgccga
     6541 tggccgaagc gcgcagggtc agccaccaat gtttaaaaac atcgcgcgcg ccctgccaga
     6601 aaccttcgcc caactcctgg gtttcggcga ctttggttct gcgcgcagcg agatcgatgc
     6661 attccgggat ggcgaagacg ccgagagcga ccaggaccag atcgatgcct tcgatcaaat
     6721 aagcggtgtc gaaggtgaag cgttcgatgc ctgacttatc atcggcgccg acggcggcga
     6781 ttaggatgcc gagaattccc gcgagcaggc ctttgagcgg cacgttgccg ctcaagacac
     6841 cgaccatggt gacgccccaa aacgtcaaca tgaaaaattc cggcgagccg aaggataaca
     6901 ccatgggacg gagaaacggc agcgacgcga tgagaacgat cggtccaatc agaccgccga
     6961 ccaacgaagc gaaaaaggac gcgctcaaag cgcgcatggc ttggccctgc ttggcgagcg
     7021 gatagccgtc cagcacggtc gcttgcgatc cagagccgcc cgggaccgag atcaacaccg
     7081 cggtgaaggc gctggcggtg ttgccgacgg catcgaagcc gacgagcagg gcgatgacgg
     7141 cgtagggatc cttcatcgtc atcacgaagg gcagcatgat ggcgagggtg ctcggtccgc
     7201 cgatgccggg caggaatccg acgagactgc cgagcaacac gccgaggcac atcagtgcca
     7261 gggaattggg tgtcagaacc tgatacagcc cttcaagggc ggcttcgatc atcgattagg
     7321 attctgatgc ggcaccgccg gccggcaggc cgtatttttt gcgaatctgc gtgagaaaat
     7381 cctgcgccgg tttcgggatc gaggtgagtt tcttgatcaa cgcctcggtc tgctcgccag
     7441 agctgagcga ccaagactcg ccgaaaaatt tcgtcgccag ggatttaaac tcgtcgtctt
     7501 tattcatctt catgtagcca tcgcgcaaag cggcgacgac gttatcggga gttttgggcg
     7561 gcgccacgat gaacttgtcc aaatcttccg aattggccca tgcctggtac gcgtcccagg
     7621 aaacgccact gggctttttg ttgccgaggt attccttgaa tgtcggcacg tcacgaaaat
     7681 ccgcgcgccg ttcttcgtcc tgctggtaga tcatttcgat aaagccttct ttgcgcatgt
     7741 ccgagatcag ccgggaattt gacgtggccc aaaggtcgat ttcgccctgg cgcaaagcca
     7801 gtgaaagctc ccggctgcct ttgtagccgt aaagccaacg cagattccaa ttcagatact
     7861 ccgcgctcca gacggtcatc gacacccaag tacggatgcc gtcggtgtcg ccgacgacta
     7921 gcttgcgcga cttcacatcg gtcaaactgt ccttcagctc cttgcgcacc atcaggatgc
     7981 tgccgccgcg ggcgatggaa ccgacggcgc ggtttttacg gtgatcgtat ttgatgcgcg
     8041 agccgccgcg attcatgttg ccaatggcgg aagatgagtc ttgcaacaac gtcaagccgt
     8101 cgggtttggc gttggcgtag aaatggttgt tggcgatggt gccgccgccg ccgggcatgt
     8161 tctgcacgac ggtgaccggg ttgccgggca agtacttggg caggaattgc gaaatcaaac
     8221 ggccggcggt gtcggtgccg ccgccggggc tggacgagat gaggatcgtt agagtcttgc
     8281 cttgaaacgt gaccggggcg ttggcggcga tcactgtcga tgtcgaagcg gcaaaaccta
     8341 tctgcccgac gagcagaaat aaatgtacaa tttttttcga ttgcatattt tatccgctga
     8401 tttatcgacg ccccatccca gcctttctcg acgaggggaa tggcatcgga cgggcggcgg
     8461 ttgtgaatcg tccatggcac gagaaaatgc gctaatcatc gctaaagcta agttcgataa
     8521 agattgtcga tgaagccgct gtcgtcgatg cggcgcagat gatggaagtc ccaaagcgcc
     8581 atggggtcgc acttctcggc ggctttgttt tgctcgatgg cgatttcata aacgttggag
     8641 atcgccggca gggtcgcgta gggtttggct tgcaagatca gtttcaaatc gtcgtataga
     8701 tggcttgccg tttcgtcgtc gagctcgcct tcggctttgt atttgtcttt gataatcttg
     8761 atgcactcgg cgcgatgggt tttgaaatag gctatgcctt cgcagacgcc gcggagcatc
     8821 ttatcgacga tgtcggggtg tttttcgacg aacatcgagc tgctcgacac ggtggtgaac
     8881 cacaccatcg gcaagaacga cacgtcaatc actttcagac cggcgcgctt tgacaaaata
     8941 tccgccggtg gcgtgacgaa ggcggcatcg gcttcaccgt cgcgcaccgc ttcccagtgg
     9001 aatttgccgc ccttggtttt ctcgacgcgg caatcgttga gcaggcctgc ttgtttgata
     9061 aacagccaag tattcaatcc cgggtgattg cccttggtgg cgatgtgttt gcctttcaag
     9121 tcggccaatt tgttgatgcc ggagtcggcg cggacgacca aacgatgatt caacaggctg
     9181 acggtttggc cgaggtagac ccatttgtcg ccggtcggac ggcggatgta gggcgagaga
     9241 tggttgccgc cgacgaactc gacggagccg ttggcgacgt tgctatgcga gtctgccgag
     9301 gtgatctcgt agtcgtaatt cacgtccagt ccatgtttgg cccaagagcc cgattcatgg
     9361 acgacgtgga ggaacggcaa gtggctgtcc gatcgatagg ggaaatttac cttatccata
     9421 gggaaaccct ccttgatgag ctgagactat ttcttgtccg ggttggcgtc aaccgccggt
     9481 cattagataa aagcacgatg gtaaatcgac ttaacaatac gtgaatcgat ttgttcgttg
     9541 ccggtacgac tcggaaaaaa ccgctgggtt cccgctaatc attttttccc cgcggccccg
     9601 cgcgctaatt cgtcatcgat gacccgtgcg aaggtttcaa gcgattgggc gccggaaact
     9661 aaccggccgt tgataaaaaa tgccggcgtg ccgctgacac cggcgcgagt gccgtcctcg
     9721 atatcttgct taatcgcggc ttgatacttg ccgctaccca ggcaggtttc aa
//
LOCUS       DASQKS010000317         5134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1424666, whole genome shotgun sequence.
ACCESSION   DASQKS010000317 DASQKS010000000
VERSION     DASQKS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424666"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..2264
                     /locus_tag="VE170_08285"
     CDS             567..2264
                     /locus_tag="VE170_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15473.1"
                     /translation="MAYDLVIRGGRVVDGSGLPSYVADVGVKDGKVVEVGKLKDGAAK
                     TIDAAGLAVSPGFIDHHTHLDAQMLWDPYGTCEPQHGVTSVVMGNCGLSLAPVRPGIE
                     DAIVKSFIRVEAIPRIALEQGVTWNWHSYGEYLDNFEGKVGINVGGLVGHIAVRHHAM
                     GEEAVERKATGTEVQKMKNLVLDAMEGGALGMSTNRNDRHMREDGKPVSSRLADDDEF
                     FALCDVLGERNAGVIETILGRNKVEHFEWYHELARRTQRPILWQSLQHRWSEPNLWRE
                     QLAAVEPIFKAGYQAYGLTHTVPLVRHFTLKDCQIFDEFPTWKNLMFLPVEVRKQAFA
                     DAATRVKLQYEIDNLRLTNYHKRWDIPKVEKCFKPENQKYVGKTIAEMAVMRNQTPID
                     AFLDLALDENLETIFWNANNGGDWNAMGEILRSPYVLIGTSDAGAHVLFGADFGYGTI
                     LLGLWVRERQVMSLEQAIHKLTFHPASIFGLQGRGLLRPGYAADIAIFDPQTVNAEEP
                     TWANDFPANTKRMVQQSIGMHYTIVNGTVINDQGRMTGDLPGQVMRGPLYRQHKAAA"
     gene            2266..2595
                     /locus_tag="VE170_08290"
     CDS             2266..2595
                     /locus_tag="VE170_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15474.1"
                     /translation="MGVSYIPVARIGELAPGAMKEVDLGGRQVLLAFAEGKYYAFARQ
                     CPHEEADLKEAGQLFNLNGPQIRCNNHSYCFDLKTGDCTLPKGGVPLTVLPTEERGEE
                     ICIRLEW"
     gene            2654..3562
                     /locus_tag="VE170_08295"
     CDS             2654..3562
                     /locus_tag="VE170_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HYJ15475.1"
                     /translation="MKPTRQNRLLSIALQLLLALILAAVSGRFTKLSAQERSFLWKVQ
                     GSENAVYILGSIHLLKRDSAALKPIVQEVFSKSKRLVLEIDLLNEGPAKFQQLVMQKG
                     VNLDGKLLASQLSPETYALTARRADELGVDLKLLAPLKPWVVALTMVVMQLQKLGYDP
                     SLGIDHQLAQRAKQANKPVAGLETAEFQMDIFNQLTLAQQEMFLRQSLLEMELLAEAV
                     EDMVGAWNSGDVERAEKLFLASLRTFPELKEQVLDRRNRRWLPQLEQFLKQSDDILVV
                     VGTAHLLGAQGIIELLKQRGYKLEQM"
     gene            3562..4056
                     /locus_tag="VE170_08300"
     CDS             3562..4056
                     /locus_tag="VE170_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ15476.1"
                     /translation="MAERNQQLKSMQVSDWMTENVLAVDTYDSIAIAREVMAKHRINQ
                     LPVLDNDLLVGIVTDRDIRDAYPTSMMIDQTEAIDSFAEKVTVEEVMSHDVLIVQPET
                     PLATAVGLLRKHRIGSLPVVKNKTLMGIITRSDILDYVLDGGPKRKRLLAKAPAVKAK
                     KQRK"
     gene            4098..4592
                     /locus_tag="VE170_08305"
     CDS             4098..4592
                     /locus_tag="VE170_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HYJ15477.1"
                     /translation="MPSFDIVSRIDMQEVDNAVNITSKTISTRYDFRASKTEISLDKK
                     EKKLLVTTEDDMRMRAIQDTLIENLVKRKVDRRCLDPKTTEPAAYGMIKREIAIKDGV
                     DSDNARAIVKLIKDKKLKVQAAMQDNQVRVTGKKIDDLQEVMQMLRGSDIPIPLQFVN
                     MQSR"
     gene            4620..5015
                     /gene="msrB"
                     /locus_tag="VE170_08310"
     CDS             4620..5015
                     /gene="msrB"
                     /locus_tag="VE170_08310"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYJ15478.1"
                     /translation="MEKIKKNDDEWKKQLSGEQFEVARKKGTERAFSGAYWDNHEKGT
                     YRCVCCGNELFSSDTKFDSGTGWPSFYAPVAAENVETETDNSYGMRRVEALCSKCDAH
                     LGHVFDDGPKPTNLRYCINSASLKFDKDK"
BASE COUNT         1211 a         1396 c         1491 g         1036 t
ORIGIN      
        1 caaatcgcac gctcagccag cgcccggcca cttactaaaa gagtgagccg ggcgctgcta
       61 tttcgggatc tgcgccgcgt atgaaacgag cgctcaaaag aatagcgatg cggcgggtca
      121 tttctatttg ccttccgcct agtgttgatt gggataaagc atcgggcgtt gcgctgcgca
      181 ggcgctcaga attaaggaaa acatcgccgc ggcaagaaat gttttgcttt tttatgtcca
      241 gttaaacttt ctcaaccata atcatgtcgc tggcgcgggc gcaataaaaa cgtttcgccg
      301 tatctgccgc cgtgctgcaa ggcgatgtta ggccgggcaa atcgtcagct ctttatgggt
      361 ttggataacg aattgtccct ggctgaggaa cttgagcatg cttccgacgg ggaaattacc
      421 ggctatatca gaatgaccgc gtgcgcgtgc tcaaccggtt gaccgcctgc ttgtcatttc
      481 gtatgttacg cagagtaata ggctattatt ggcgaacggc gaattgacac aatccttaac
      541 tctccaactc aacaggaggt tttcttatgg cttatgatct cgtaatacgc ggtggacggg
      601 tggtcgacgg ttccggcttg ccgtcctacg tagccgatgt cggtgtcaaa gacggcaagg
      661 tcgtcgaagt gggcaagctt aaagacggcg ccgcgaaaac catcgacgcc gccgggctgg
      721 cagtgtcgcc cggtttcatc gatcatcata cccatctcga tgcccagatg ctctgggatc
      781 cctatggcac ctgcgagccg cagcacggcg tgacctcggt ggtgatgggt aactgcggct
      841 tgtccctggc accggttcgg cccggcatcg aagacgccat cgtcaagagc ttcatccgcg
      901 tcgaggcgat tccccggatt gcgctcgaac agggcgtgac gtggaactgg catagttacg
      961 gcgaatatct tgacaacttc gagggcaagg tcggcatcaa tgtcggcggc ctggtcggcc
     1021 acatcgccgt gcgccaccac gccatgggcg aagaggcggt ggaacgcaag gccaccggca
     1081 ctgaagtgca aaagatgaaa aacctggtgc tcgatgccat ggaaggcggc gccttgggta
     1141 tgtcgaccaa ccgcaacgac cgccacatgc gcgaagacgg caagccggtg tcgagccgct
     1201 tggccgatga tgacgaattt tttgcgctat gcgacgtcct gggcgagcgc aacgccggcg
     1261 tcatcgaaac catcctcggt cgcaacaagg tcgaacattt cgagtggtac catgaattgg
     1321 caaggcggac tcagagaccg atcctttggc agagcctgca acatcgctgg tccgaaccca
     1381 acctctggcg cgagcagttg gccgccgtcg agccgatctt caaagccggc tatcaagcct
     1441 acggcttgac ccatacggtg ccgctggtgc gccatttcac gctgaaagac tgtcagatct
     1501 tcgacgagtt cccgacttgg aaaaatttga tgtttctgcc ggttgaagtg cgcaagcagg
     1561 cgtttgccga tgccgcgacg cgggtaaagc tgcagtacga gatcgataac ctgcgcttga
     1621 ccaactacca caagcgttgg gatattccca aagtcgaaaa atgcttcaag ccggaaaatc
     1681 aaaaatatgt cggcaagacc atcgccgaga tggcggtcat gcgcaatcaa acgccaatcg
     1741 atgcgtttct cgatctcgcc ctcgacgaaa atttggagac gattttctgg aacgcgaaca
     1801 acggcggcga ctggaacgcc atgggtgaaa ttctccgcag cccctatgtg ctgatcggca
     1861 cttcggacgc cggcgcccat gtgctgttcg gtgcggactt cggttacggc acgatcctct
     1921 tgggcctgtg ggtgcgcgag cgccaggtga tgtcgctgga gcaggcgatt cataaactga
     1981 cctttcatcc ggcgtcgatc ttcggcttgc aggggcgcgg gttattgcgg ccgggctacg
     2041 ccgccgatat cgcgatcttt gacccccaaa cggtcaacgc cgaggagcct acgtgggcca
     2101 atgatttccc ggccaacacc aaacgcatgg tgcagcaatc gatcggcatg cactacacca
     2161 tcgtcaacgg cactgtgatc aatgatcaag ggcgcatgac cggagatctg cccggccaag
     2221 tgatgcgggg gccgctctac cgtcaacata aggcggccgc gtaatatggg agtgagttat
     2281 attccagttg cgcgcatcgg tgagctcgct cctggtgcca tgaaagaggt cgacctcggc
     2341 ggacgccaag tgctcttggc gttcgccgag ggtaagtatt atgcgttcgc gcgccagtgt
     2401 cctcatgaag aagcggattt gaaagaggcc gggcagctgt tcaatctcaa tggcccgcag
     2461 atccgctgca acaatcatag ctactgtttc gatctgaaaa ccggcgactg cacgctgccc
     2521 aaaggcggcg tgcccctgac cgtgctgcct accgaagaac gcggcgaaga aatttgcatt
     2581 cgcctcgagt ggtgaagctt ctggcgaatg ccggcacgac cctcatttgt cgctcatcgc
     2641 cggcgcattc acaatgaaac cgacgcgaca gaatcgcctg ttatcgatcg cgcttcagtt
     2701 actgctcgcg ctgattttgg cggccgtgag cgggcgattt accaagcttt cggcgcagga
     2761 gcgaagtttt ctctggaagg tgcagggaag cgaaaacgcg gtttacatcc tcgggtcgat
     2821 tcatctgctc aaacgcgaca gtgcggcgtt gaaaccgatc gtgcaggagg tttttagcaa
     2881 gagtaagcga ttggtcttgg aaatagattt actcaacgag ggaccagcga agtttcagca
     2941 gttggtaatg caaaaagggg tcaatctcga cggtaaattg ctagcctcgc agttgtcgcc
     3001 ggagacctat gcattgacgg cgcgacgggc cgatgaactg ggcgtcgatc tcaaactgct
     3061 ggcgccgctc aaaccttggg tggtcgcgct gacgatggtg gtgatgcagc tgcagaaact
     3121 cggctacgat ccgagcctgg gcattgatca tcaattggcg caacgcgcca agcaggcgaa
     3181 caaaccggtt gccggtttgg aaaccgccga gtttcagatg gacatcttca accagctgac
     3241 actggcgcaa caggagatgt tcctacgcca atcgttgctt gaaatggagt tgctggccga
     3301 agcggtcgag gacatggtgg gcgcgtggaa cagcggcgat gtcgagcgcg ccgaaaagct
     3361 ttttttagca agcctgcgca ccttccctga gctgaaggag caggtactcg atcggcgcaa
     3421 ccgtcggtgg ctaccgcagc tcgagcagtt cttaaagcaa agcgacgata ttctcgtcgt
     3481 ggttggcacc gcgcacttgc tcggggccca gggcatcatc gagttactca agcaacgtgg
     3541 ctacaaactg gagcaaatgt aatggctgaa cggaaccaac agctgaaatc gatgcaggtg
     3601 tccgattgga tgaccgagaa cgtgctggcc gtggatacct acgacagcat cgccatcgcg
     3661 cgcgaggtca tggccaagca ccggatcaat caactgccgg tgctcgataa cgatttactt
     3721 gtcggtatcg tcaccgaccg ggatattcgc gatgcctatc cgaccagcat gatgatcgac
     3781 cagaccgagg cgatcgacag cttcgccgag aaagtcaccg tcgaagaagt gatgagccat
     3841 gatgtcttga tcgtgcagcc ggaaacgcct ctggccacgg ctgtcggctt gctgcgcaaa
     3901 caccgcatcg gctctttgcc ggtggtcaaa aacaagacgc tcatgggcat catcacgcgc
     3961 agcgatattc tcgattatgt gctcgacggc gggccgaagc gcaaaagatt gctggcgaaa
     4021 gcgccggctg tcaaagcaaa aaaacagcgc aaatagttac gctccgcgcc ttcatagtta
     4081 tccgaaggga gggttttatg ccgtctttcg atatcgtcag ccgcatcgat atgcaggagg
     4141 tcgacaacgc ggttaacatc accagtaaaa caatttcgac ccgctatgat tttcgcgctt
     4201 caaaaaccga aatttctctg gacaagaagg agaaaaaact tctggttacc acagaagacg
     4261 acatgcggat gcgggcgatc caggacactt tgatcgagaa cctggtcaag cgcaaagtcg
     4321 accgccgctg tctcgacccc aaaacaaccg agccggcggc ctacggcatg attaaacgtg
     4381 aaatcgcgat caaggacggc gtcgactcgg acaacgcccg cgccatcgtc aaattgatca
     4441 aggacaagaa actcaaagtc caagccgcca tgcaggataa ccaggtgcgc gtgacgggaa
     4501 aaaaaatcga cgatctccag gaagtgatgc agatgctgcg cggctcggat attcccatcc
     4561 ccttgcagtt cgtcaatatg cagagccgtt gaaatatatt tgattggata ggagccgcca
     4621 tggaaaaaat caagaaaaac gacgatgaat ggaagaaaca attgagcggc gagcaattcg
     4681 aggtcgctcg caagaagggc accgagaggg ctttctccgg cgcctattgg gacaaccacg
     4741 agaagggcac ctaccgctgc gtttgctgcg gcaatgagct attcagctcg gacaccaagt
     4801 tcgactccgg caccggctgg ccaagctttt atgctccagt ggcggcggaa aacgtcgaga
     4861 ccgaaactga caacagctat ggcatgcgcc gggtcgaagc gctgtgcagc aagtgcgacg
     4921 cccacttggg ccatgtcttc gatgacggcc ccaagccgac gaatctgcgc tattgcatca
     4981 attcggcatc gctcaagttc gacaaagaca agtagcatga gcgccagcgc ttttcaggac
     5041 cagggctcgg tgcatcactg ccatggctgc ggcgccgcca atgaaaaggg attgcagctg
     5101 aaaagttact gggatggcga tagtgcggtc gccc
//
LOCUS       DASQKS010000318         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3533738, whole genome shotgun sequence.
ACCESSION   DASQKS010000318 DASQKS010000000
VERSION     DASQKS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533738"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VE170_08315"
     CDS             complement(<1..522)
                     /locus_tag="VE170_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ15479.1"
                     /translation="MDSENVKGGASNGHSDLEAAVLQRYGNAAEEVEACLCLPVSYDK
                     ALLKVIPDEIIEKDYGCGDPSRYVRPGETVLDLGSGSGKACFIISQIVGAKGKVIGVD
                     FNPPMLELARKHQNAIGDKIGFHNVDFRRGKIQDLKTNLELVDQYLIHNPVTSVSDLA
                     KLESYTDKIRRELP"
     gene            complement(724..1828)
                     /gene="prfB"
                     /locus_tag="VE170_08320"
     CDS             complement(join(724..1770,1772..1828))
                     /gene="prfB"
                     /locus_tag="VE170_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HYJ15480.1"
                     /translation="MIEETTEQLTHLEGRLEQLGGGFDVDSKQKRVEELSQITAQPDF
                     WNDADKAQTVLKEQSALKAVLSSWEKHQADLEEARFFLDVARDEKSEDALGEAAGKVT
                     AITKEMAQTELTQLLGEPDDRRNALVTLHPGAGGTEAQDWAEILMRMYLRWCDRRGFR
                     KEILDYQPGEEAGVKNVTFNVTGDYAYGYLKAEAGIHRLVRISPFDANSRRHTSFASV
                     FVYPEVDDDIKIEINDDDLRVDTYRSSGAGGQHVNKTDSAVRLTHLPTNIVVACQNER
                     SQHKNRAMAMKILKSRLYELEVKKQKEKMESYHKTKKDIAWGSQIRSYVLAPYRLVKD
                     HRTNVEVGNADAVLDGDLDQFIEAYLLMADEPV"
     gene            complement(1884..2327)
                     /gene="ybeY"
                     /locus_tag="VE170_08325"
     CDS             complement(1884..2327)
                     /gene="ybeY"
                     /locus_tag="VE170_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYJ15481.1"
                     /translation="MAVEITRLGAGTKYPTAALRKIAASLLRAVKQSRAELSIALVGN
                     REMRPLNAKYRKKNQTTDVLSFLVEDQPVSGGKILGDVVISVEQARRQAKERGKTLKS
                     EMVTLLIHGILHLLGYDHEISPRQARIMFAYERKLYAHLCDRGLL"
     gene            complement(2429..>2930)
                     /locus_tag="VE170_08330"
     CDS             complement(2429..>2930)
                     /locus_tag="VE170_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15482.1"
                     /translation="DEYAWYSVLLDGMGKAKGMDFMRALARQQLHIPGGSSIMRLQLM
                     LAGESSMVIAARGRRATEYKEKGAPVDYRLLDPYPAEPSGFALMRRAAHPHASILFID
                     WMLSEEGQTVLALQVPRITLRKGVKQIPRHQELYKKDFVYVNPAYIGANLTELIASYQ
                     QVFNIR"
BASE COUNT          582 a          842 c          830 g          676 t
ORIGIN      
        1 cggcagctcg cgcctgattt tatcggtgta gctttcgagc ttggcgagat cgctaaccga
       61 ggtgacggga ttatgaatca gatattggtc gaccagctct aaattggttt tgagatcctg
      121 aatcttaccg cggcggaaat cgacgttgtg aaagccgatc ttgtcgccga tagcgttctg
      181 gtgtttgcgc gccagttcca acattggcgg attgaaatcg acgccgatga ctttgccctt
      241 ggcgccgaca atttgcgaaa taatgaagca agccttgccg ctccccgaac cgagatcgag
      301 aacggtctcg ccgggacgaa cgtaacgcga cggatcgccg cagccgtaat ctttttcgat
      361 gatttcgtcc gggatgacct taagcaacgc cttgtcgtaa ctcaccggca ggcacaaaca
      421 agcttcgact tcctcggcgg cgttgccata gcgctgcaac accgccgctt ccaaatcact
      481 atggccattg ctcgcgccgc ccttgacatt ctccgagtcc ataacccctc ccgcgattgg
      541 cagcgaactt gatcgatgcc gagaaaaatc atcttgatcg aatcggcgca agtcaaaaac
      601 cggaaacacc cagccatcgc aattgtttag ctgtcgtcac aacgacagaa gttgccgaaa
      661 agttccgcgc ggctgggcgg agcgacgcgt tgtccctaac tccttacccc ttcctcctta
      721 aacttaaacc ggctcgtccg ccatcagcag ataggcttcg atgaattgat cgaggtcgcc
      781 gtcgagcacc gcgtcggcgt tgccgacctc gacattggtg cggtggtcct tgaccaagcg
      841 gtagggcgcg agcacgtagg agcggatctg gctgccccag gcgatgtctt tcttggtctt
      901 gtggtaggac tccatctttt ctttttgttt cttgacctcg agttcgtaga gccgcgattt
      961 tagaattttc atcgccatgg cgcggttctt atgctgcgaa cgttcgtttt gacaggcgac
     1021 cacgatattg gtcggcagat gggtcaagcg cactgccgaa tcggttttat tgacatgctg
     1081 gccgccggcg ccgctggagc ggtaggtatc gacgcgcaga tcgtcgtcgt taatttcgat
     1141 tttgatatcg tcgtcgacct cgggataaac gaagaccgaa gcaaacgagg tatggcggcg
     1201 cgagttggca tcgaagggag agattcgcac caagcgatga ataccggctt cggctttgag
     1261 atatccgtaa gcgtagtcgc cggttacgtt gaacgtaacg tttttgaccc ccgcttcctc
     1321 gcccggctga tagtcgagta tctctttgcg aaaaccgcgc cggtcgcacc agcgcagata
     1381 catgcgcatg agaatctcgg cccagtcctg cgcctcggta ccgccggcgc cgggatgaag
     1441 cgtcaccagc gcgttacgcc ggtcgtcggg ctcgccaagc agctgcgtca gctcggtctg
     1501 ggccatctcc ttggtgatcg ccgtgacttt gccggcggct tcgccgaggg cgtcttcgct
     1561 tttctcgtct ctcgcgacgt cgaggaaaaa ccgcgcctct tcaagatcgg cttgatgttt
     1621 ttcccaactg gagagaaccg ccttgagggc ggactgttct ttgagaacgg tttgagcttt
     1681 gtcagcgtcg ttccagaaat ccggctgggc ggtgatttgg ctcagctctt cgacgcgctt
     1741 ctgcttgctg tcgacgtcaa agccgcctcc gcaactgctc aagacgccct tcgaggtggg
     1801 taagttgttc ggtggtttct tcgatcatgg cttctttctt tggccgatta gccctgagga
     1861 ctgctgactg tccggcatta tagctacaat aacccgcggt cacaaaggtg agcgtaaagt
     1921 ttccgctcgt aagcgaacat gatgcgcgcc tggcgcggcg atatttcatg atcgtaaccg
     1981 agcaggtgca ggatgccgtg aatcagtaga gtcaccatct cgctcttgag cgtcttgccg
     2041 cgctccttcg cctggcgccg tgcctgttcg accgagatca ccacgtcgcc gaggatttta
     2101 ccccccgaca ccggctggtc ctcgaccaag aaggacagca cgtcggtggt ttggtttttt
     2161 ttacgatact tggcgttgag cggccgcatt tcccggttgc ccaccagagc gatgctgagc
     2221 tcggcacggc tttgcttgac ggctcgaagc aggctggcgg cgatcttgcg cagcgccgcg
     2281 gtcggatact tggtcccggc gcccaaccgg gtgatttcaa cggccatgct tattaaatta
     2341 tcagcggacc taggtcattt cgagtccaac gagcaatccc gcccagccgc ctagctgagg
     2401 caaaaactag ttgaaccacg agccgcgctt agcggatgtt aaaaacctgc tggtaggaag
     2461 cgatcaattc ggtcaagtta gcgccgatat aggctggatt gacgtagacg aaatcttttt
     2521 tgtaaagctc ctgatggcgg ggaatctgtt tgacgccttt acgtaaggta atccgcggca
     2581 cctgcagggc caacaccgtc tggccttctt cggaaagcat ccagtcgatg aacagtatcg
     2641 aggcatgcgg atgggccgcc cggcgcatca aagcaaaacc gctgggctcg gcgggataag
     2701 gatcgagcag acgatagtcc accggcgcgc ccttctcttt atattcggtg gcgcgccggc
     2761 cccgcgccgc gatcaccatc gaggattcgc cggcgagcat caactgcaag cgcatgatgc
     2821 tgctgccgcc cgggatatgc agctgctggc gcgccagcgc gcgcataaag tccataccct
     2881 tggccttgcc catgccgtcg agcagcaccg aataccaggc gtattcatcg
//
LOCUS       DASQKS010000319         6158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3942353, whole genome shotgun sequence.
ACCESSION   DASQKS010000319 DASQKS010000000
VERSION     DASQKS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3942353"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VE170_08335"
     CDS             <1..670
                     /locus_tag="VE170_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYJ15483.1"
                     /translation="ICSVGPQKLMPVPKYMVEGYDISEISAPTGLEPFFHGKPPKLWS
                     VGDTAILDRRLLGIISARRIDTDLALRSSQLLKQLAFLENIAFISGWHSPMEDEALRI
                     LSANSVRIVFCLSKALNRFRPSLQVKNEINDGRVLLLTHCSLKAKRISRDASLRRTHL
                     VVGLAATVLVLSAPEGSASLELARSALRQGKPVYTLEHRLNKQLLASGALSATLETID
                     ESLR"
     gene            complement(869..1702)
                     /locus_tag="VE170_08340"
     CDS             complement(869..1702)
                     /locus_tag="VE170_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ15484.1"
                     /translation="MSWRQLWHQFRKNRAAQLSLYLIIAFVLVASLADPLSPYDPFAL
                     GDETLIAPRGDHWMGTDDLGRDTLSRVIHGSRVPLLVGFLATAASMALGILIGSLSGY
                     FGGRTDIVLMRVTEYVLVVPRFFLALLVVAMLGTGIEKIILVIGILGWPEVARVVRAQ
                     FLTFREREFVIAARAIGASHAKVIFREILPNAIPPAVVVASILVARAILLEAGLSFLG
                     LGDPNLISWGSLLSEAQERISASVWLALFPGLAISLLVLCINLFGDGINDVLNPKLRK
                     K"
     gene            complement(1948..2922)
                     /locus_tag="VE170_08345"
     CDS             complement(1948..2922)
                     /locus_tag="VE170_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ15485.1"
                     /translation="MPILTFVLRRLLHALPLLVCVIVFNFFLIHLAPGDPIQALVGEF
                     PAPAAYLVEMRKLFGLDQPLYIQLLLYIRNILSGDLGFSFYYRQPVLNVIMDRVPATL
                     QLMLPALVFSSAVGILLGILSARRPYSFADNAISTLSLFGYCVPAFWFGQMLMAAFAI
                     ELGWLPSQGISTVGVDLSGSALVLDRAAHLVLPFTALAIRHLAVNARMMRSSMLEVAY
                     EDFVTVARAKGLHEKDVITRHMVPNALMPVVTIIGVDVGFLFTGSVLVETVFGWPGIG
                     RLMYESIVKRDYPVLMGNFLMTTALVVVVNLLVDLIYLWLDPRVKYQK"
     gene            complement(3007..4716)
                     /locus_tag="VE170_08350"
     CDS             complement(3007..4716)
                     /locus_tag="VE170_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15486.1"
                     /translation="MAKFLLLSRGVNMQILIARKLPERRRCSLLAGFFALALALLLAL
                     AHPDAWAGPVEGSPRRGGTLILGMPADPSTLNCGIESSQIVAMVTSNIYNGLLHLDEE
                     SNPHPDLAQSWDISPDGLVYTFQLRDNVKWHDGQPLTSADVKFSFENLVGKYNARGRE
                     AYRNIKAIETPNATTVKISLKKVYSPFLEILTAHDGCIMPKHIYEIGDVFKNPRNNDQ
                     PIGSGPFKFKEWQKGSHITLVRNENYFKKGRPFLDSVIYRIIPNAATRAIAFETGEIN
                     AIFASNSFPYQHVDRLKKLKNAVIKDIGSPSLIGVNFNLKGNPILAKKEVRVAIAHAI
                     DKKFVVEKGLRAVGKVIDSVIPPGIPWAYNAGVPKYPFDLKKANALLDEAGHARGAGG
                     TRFALKLSFEAGNDNAERPVQILREQLKAAGIDIKLERLERSVMLEKIFQKYDFDLWF
                     GPLTTRGHPALGTARLYATSSITGQPFTNFTRYSNPKVDQLFEQAVAATKKADMVKAY
                     YEVQEVLMRDLPTIPVADRLQLNIIKDQFRGALTSTETYERMDEIWWVKGTALKDGDF
                     ERE"
     gene            complement(4694..6055)
                     /locus_tag="VE170_08355"
     CDS             complement(4694..6055)
                     /locus_tag="VE170_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ15487.1"
                     /translation="MGPTATLAQFVVDTRLRDIPTRAVEISKLVILDVLGVTLGASRQ
                     SLARILNEYLREAAAAPRAGVIGSPIRTEPALAAWANGALAFALDFDDNIHGSTHTVP
                     AALAIGEGNGASGATLLEAYILGRDICFRLDAALDAGRRTNQGGPTSRGWFAGGTTGS
                     LAAAAAAGKVLRLDAKQMATAFGIAVAGAGGVRRNFGTMAKALQTGNAARNGVTAALL
                     AQKGFSADQTILEAPMGLVNALCLEGECDWDALTQDLGKYFYMEKMPPIKRFPTCTPA
                     HKPIEGLLALRQHHGFTVDEVESVESDFHERSLARTDPREAIAGPNSMPFILALALLD
                     GKVALEQFTDQNIRAPRVRAAMAKIRPRANPRQPGQAEPPDRITVTLKNGTVHTIEVA
                     ERTTLHSYAEIHGKFFDCATLALNRGSADELARLIDRLESVDDVGALMRCMNGNNGKI
                     FTA"
BASE COUNT         1384 a         1687 c         1800 g         1287 t
ORIGIN      
        1 aatttgcagt gtcggcccgc aaaagctcat gcctgtgccg aaatatatgg tcgaaggtta
       61 tgacatctcg gaaattagtg ccccgacggg tctcgaacca ttttttcatg gcaaaccgcc
      121 gaagctttgg agtgtaggag atacggcgat cttagaccgc agacttctcg gaatcatttc
      181 cgctcgacgg atcgatactg atctggcatt aagaagctca caactcttga aacaattggc
      241 attcttggaa aacatcgcct tcataagtgg ctggcattct ccaatggaag acgaggccct
      301 gcgtattctg tccgctaact ccgtgcggat cgttttctgt ctttcgaaag ctttaaatcg
      361 attcagaccc tcgttgcagg taaagaatga aattaacgac ggtcgcgtgc tcttgctaac
      421 tcactgtagc ctcaaagcga aaaggatcag ccgtgatgca tccttaaggc gcactcatct
      481 tgtcgtaggg ttggcagcga cggtgttagt tttatccgcc ccggagggaa gcgcttcgtt
      541 ggagttggca agatccgcgt tgcgccaagg aaagcccgtg tacactttgg agcaccgtct
      601 gaacaagcag ctgttggcgt ccggcgcgtt atctgcgacg ttggaaacaa tcgacgagtc
      661 tctgcggtag aaaaatatgc agtaacaaag acgcttgagt acatcatttc tgaacaggca
      721 ccgaatgtct gcgagcagat tgaggcgacg gtggcatttg ccgactccga gggagacctt
      781 gccatggccg cggcagcgcg gcacccagtt caagtggcaa gacgggcgcg gcgtgattcg
      841 tcgtctgaga acattcccca attgccagtt atttctttct gagcttggga ttgagcacgt
      901 cattaatgcc atcgccgaac agattgatgc acagcacgag cagtgaaatg gctaagccgg
      961 gaaagagcgc cagccagacc gatgcgctga tccgttcctg agcttcgctc aacagcgagc
     1021 cccagctgat caagttggga tcgcctaaac caagaaaact caaccccgcc tctaagagaa
     1081 tcgcccgcgc gactaatatc gacgccacca ccaccgccgg cggaatcgca ttggggagaa
     1141 tttcccggaa aataactttt gcatggctgg cgccgatggc gcgggcagcg atcacaaatt
     1201 cgcgctcgcg aaaggtgaga aattgcgcgc gcacgacccg ggccacttcc ggccagccca
     1261 ggatgccgat gacaaggata attttttcaa tgccggtgcc gagcatggcg accaccaaca
     1321 gggcgagaaa aaatcgcggc acgaccaaga cgtactcggt gacgcgcatc aagacgatat
     1381 cggtccggcc gccaaaatag ccggacagcg aaccgatcag gatccctagg gccatcgacg
     1441 cagcggtggc gagaaagccg accagcagcg gcacgcggct gccatggatc acccggctca
     1501 gggtatcgcg gccgagatcg tcggtgccca tccagtgatc accgcgcggc gcgattaatg
     1561 tctcatcgcc gagggcaaag ggatcgtacg gcgaaagcgg gtctgcgagg gacgcgacga
     1621 gaacgaaagc gatgatcaga tacaggctca gttgtgccgc gcggtttttg cgaaactgat
     1681 gccagagttg acgccaagac atgggttagc cgggattact cgccgttaaa atacgatcgc
     1741 gatgtaattc ttcctgaacc aaggcaaagc tgctgcgccg tgagggtgcg gcaagtcgct
     1801 tatgagatca gcagcgaaaa ggtcaaacat cgcgccgacc gccgaactct ccaatgaaac
     1861 tgttgcgcta gtgcgtttgt tcctcaaggc gacatctcga tcggtacaca gccatgagtt
     1921 cacgtttaat atcagcccgg attgagctca cttctggtat ttcacccgcg gatcgagcca
     1981 aaggtagatc aagtcgacaa gcagattcac aactactacc agcgcggtgg tcatgagaaa
     2041 atttcccatc aagaccgggt agtcgcgctt gacaatagac tcgtacatca gccggccgat
     2101 gccgggccag ccgaaaacgg tttcgactaa gaccgacccg gtgaaaagaa aaccgacatc
     2161 gacaccgatg atggttacca ccggcatcaa ggcattgggc accatatggc gcgtgatgac
     2221 atctttttca tgcaagcctt tagcgcgggc gacagttacg aaatcttcgt aagccacttc
     2281 cagcatgctg ctgcgcatca tgcgcgcatt cacggcaaga tggcggatcg ccaaagcggt
     2341 aaacggcagg acgaggtggg ctgcgcggtc gagtacgagc gcgctgccgc tcagatcgac
     2401 accgacggtg ctaatacctt gcgacggcaa ccagccgagc tctatggcga aggccgccat
     2461 cagcatttgg ccgaaccaga acgccggcac gcaatagccg aagagcgaca aagtgctgat
     2521 ggcattatcc gcgaacgaat acggtctacg ggcggacaaa atgcccaaaa gaatccccac
     2581 ggcggacgag aagaccagtg ccggcagcat caattgcaat gtcgccggta cgcggtccat
     2641 gatgacgttc agtaccggct ggcgatagta aaaagaaaaa cccaaatcgc cggaaagtat
     2701 atttctgatg tagagcagca gttgaatgta cagcggctgg tcgagaccga aaagtttgcg
     2761 catctcgacg agataggccg ccggcgcggg gaattcgccg accaacgctt gaatcggatc
     2821 gcccggggcc aggtgaatca ggaagaaatt aaaaactatg acgcaaacca acaacggcag
     2881 ggcatggagc aaacggcgca ggacgaaagt caggatcggc atggtcgctt cgctaaacgc
     2941 tcgcgattgc agatttcaca tccagatttt aaattgccag actcgaactg gacgttagtt
     3001 gagcaatcat tcgcgctcga agtcgccgtc ttttagcgcc gtgcctttga cccaccagat
     3061 ctcgtccatg cgttcgtagg tctcggtgct ggtgagggcg ccgcgaaatt gatccttgat
     3121 gatgttcagc tgcagccggt cggccaccgg gatggtcggc agatcgcgca ttaaaacctc
     3181 ttgaacctca tagtaggctt tgaccatgtc ggcttttttg gtcgcggcca ccgcttgctc
     3241 gaacagttgg tcgaccttgg gattactgta gcgcgtgaag ttggtgaacg gctggccggt
     3301 gatcgacgag gtcgcataca agcgcgcggt gccgagggcg ggatggccgc gagtggtgag
     3361 cgggccgaac cacagatcga aatcgtattt ttgaaaaatc ttctccaaca ttaccgagcg
     3421 ctcgagacgc tcaagtttaa tgtcgatgcc ggcggctttt aattgctcgc gcaagatctg
     3481 caccggcctc tcggcgttat cgttgcccgc ttcgaagcta agcttgagcg cgaagcgtgt
     3541 gccgccggcg cctctcgcat gcccggcttc atcgagcaga gcattggcct ttttcaaatc
     3601 gaagggatac ttgggcacgc cggcattata agcccagggg atgcccggcg ggatcacgct
     3661 gtcgatgact ttgccgaccg cgcgcaaacc tttttcaacc acgaatttct tgtcgatcgc
     3721 gtgggcgatg gcgacgcgga cttctttctt ggctaaaatc ggattgccct ttaaattgaa
     3781 attcacgccg atgagcgacg gcgagccgat gtctttgatg acggcgtttt tcagtttctt
     3841 gagccggtcg acatgctgat aaggaaagga attactcgca aagatcgcat tgatctcacc
     3901 cgtttcaaag gcaatggcgc gggtcgccgc attgggaatg atacggtaga tgacgctgtc
     3961 caaaaacggc cggcctttct tgaaataatt ttcattgcgc accagcgtga tgtggctgcc
     4021 tttttgccat tccttgaatt taaacggtcc gctgccgatg ggctgatcat tgttgcgcgg
     4081 atttttaaac acgtcgccga tctcatagat atgcttgggc atgatgcagc cgtcgtgggc
     4141 ggtaaggatt tcaagaaacg gcgaataaac ttttttgaga ctgatcttca ccgtggtcgc
     4201 gttgggcgtc tcgatggctt tgatgttacg gtacgcttcg cggccgcgcg cgttgtattt
     4261 gccgacgagg ttctcgaagc tgaattttac gtcggccgag gtcagcggct ggccatcgtg
     4321 ccatttgacg ttgtcgcgca gctgaaaggt atagaccaag ccgtccggtg agatgtccca
     4381 cgactgagcc aagtcgggat ggggattcga ctcctcatcg aggtgcagca ggccgttgta
     4441 aatattggac gttaccatgg cgacgatctg actgctctcg atgccgcagt tgagcgtcga
     4501 tggatcggcg ggcatgccga ggatcaaggt accaccccgg cgcgggctgc cttcgaccgg
     4561 acccgcccaa gcgtcggggt gagcaagggc gagcagaagg gcgagcgcca gtgcgaaaaa
     4621 accggcgagc aacgaacagc gacgtctttc cggcaatttt ctagcgatta aaatctgcat
     4681 attaacaccc ctgctaagca gtaaaaattt tgccattgtt accgttcata cagcgcatca
     4741 atgcaccgac gtcgtcgacg ctttcaagcc ggtcaatcaa tctggccagc tcgtcggcgc
     4801 tgccgcggtt taacgcgagc gtcgcacagt cgaagaattt gccatgaatc tctgcgtagg
     4861 agtgcagcgt cgtgcgctcg gccacttcga tggtatgaac cgtgccgttc ttgagtgtca
     4921 ccgttatgcg gtccggcggt tcggcttggc ccggctgccg tggattggcg cgaggacgga
     4981 ttttcgccat cgccgcacgc acgcgcggcg cgcgaatgtt ttggtcagtg aattgctcaa
     5041 gcgcgacctt gccgtcaagt aatgcgaggg cgagaatgaa cggcatgctg ttgggtccgg
     5101 cgatagcttc ccggggatcg gtgcgcgcca acgagcgctc atgaaagtcg ctctcgacgc
     5161 tttcgacctc atcgaccgtg aagccatggt gttggcgcag cgccaacagt ccttcgattg
     5221 gcttgtgcgc cggggtgcag gtagggaagc gcttgatcgg cggcattttt tccatgtaaa
     5281 aatatttacc caagtcttgg gtcaatgcat cccagtcgca ctcgccctcg aggcaaagcg
     5341 cgttgaccaa tcccatcggc gcttcgagaa tggtctgatc ggcgctaaaa cctttttgcg
     5401 ccagcagcgc tgcggtgacg ccgttgcgcg cggcgttgcc ggtttgcagc gccttggcca
     5461 tggtgccgaa gttgcgcctg acgccgccgg cgccggccac ggctatgccg aaagcggtcg
     5521 ccatctgttt ggcgtccaag cgcaagactt tccccgcggc ggcagctgcg gccaagctgc
     5581 cggtggtgcc gccggcaaac caaccgcgcg acgtcgggcc accttggttt gtgcgccggc
     5641 cggcatcgag cgcggcgtcg aggcgaaaac agatatcgcg tccgaggatg taggcttcca
     5701 agagcgttgc accgcttgcg ccgttgcctt cgccgatggc caacgccgcc ggcacggtgt
     5761 gagtgctgcc gtggatgtta tcgtcgaagt caagggcgaa cgcgagcgcc ccgttggccc
     5821 aagccgcgag cgccggctcg gtgcggattg gactgccaat gacacccgct cttggcgccg
     5881 ccgccgcttc gcgcaaatat tcgttcagaa tccgcgccaa actttgccgt gatgcgccga
     5941 gggtgacgcc taagacgtcg agaataacca gcttcgatat ttcaaccgcc cgagtgggaa
     6001 tgtctcgcag ccgggtgtcg acgacgaact gcgccagggt tgcagtcggg cccatggtta
     6061 ataatccttt tgcatccctt gagatgcgaa ttatgtgctc gccatcaagc agccgtattg
     6121 cggcttgggc tgccagcaat gcaacacatg caaaacat
//
LOCUS       DASQKS010000320         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2697241, whole genome shotgun sequence.
ACCESSION   DASQKS010000320 DASQKS010000000
VERSION     DASQKS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697241"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VE170_08360"
     CDS             <1..727
                     /locus_tag="VE170_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HYJ15488.1"
                     /translation="TLKKAIDAAGISEAFVSAIAPGSLEMFCRDQNSYYPTIEKFLEG
                     IAAALREEYRAIIDAGFILQLDDPGLPGAWDMLDPQPSVEEYRRYASLRVEILNHALQ
                     GIPIERVRYHICWGSWHGPHTTDFPLRDIVDIMLRVNAQAYLLEAGNVRHEHEYKVWR
                     DVKLPAGKILIPGVVSHSTDLVEHPELVADRILAFADIVGRENVIAGTDCGLGGRVHP
                     QIAWAKLRVLSEGAAIASKKLWR"
     gene            915..1559
                     /locus_tag="VE170_08365"
     CDS             915..1559
                     /locus_tag="VE170_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ15489.1"
                     /translation="MEITLYYAPHTCAVAPYITLTEAGADFEVRPLNFRKRQNYTPEY
                     LKINPKHKVPALVIDGKILTENVAIHQWVHRTFPDAKILPSDSWDQLQAISLHAWCAS
                     GIHPYLSRINNPPKVCDATGASDSVIKFATEILFENFQIGDDLLAGREYFFDHFTAPD
                     AHFFWCCRRATQLEVDISGFPNVVEHFKRMQRRPSVKKLLAYEKQVNEEFAKTA"
     gene            1825..2796
                     /locus_tag="VE170_08370"
     CDS             1825..2796
                     /locus_tag="VE170_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15490.1"
                     /translation="MPETATVIGRSGFRISALMLIVLMAHSVGCARRFPVTEVTTIKA
                     ANPAELQRHLLSRKPDVAQFKLRGPFDVTERRDLEIRLASGLTVAADLYLCAAAHKAP
                     LVIVLHGLDNSKEDHAFQAMHLASWGMHSLALDLPKQGPWITNGRTLARLVDAIRKTP
                     QLVDGRIEAEKVILVGHSFGATAVATALGAGAPALGGVLLDPAGIGRELPQLLRRITV
                     PVMVIGADEDIWPTRNRGQFYRFIPAAVGEISIRDTVHEDAQYPNHHTLRPFEDEPDD
                     TEEAQITFVSALTASAFGLAATASIDYGWNSFELAIKNGTFFNARRK"
     gene            2927..3232
                     /locus_tag="VE170_08375"
     CDS             2927..3232
                     /locus_tag="VE170_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15491.1"
                     /translation="MLEVGGVFYTLVSGVHLEGGDKLGSWYLTELENSAGRPEKVCSW
                     LKKCTCVKVTCESKSDWKRPTELIKVDKVCNDVSVPRMELLEFVAGYPPNRRFKWHP"
     gene            3569..>3912
                     /locus_tag="VE170_08380"
     CDS             3569..>3912
                     /locus_tag="VE170_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ15492.1"
                     /translation="MKFKLILLAFIYLLGTSSFAASNPFDDASAAYERGDYVHAIHLF
                     RELAAKGHQWAQRRLGSIYAQGHGVPQDYQEAVKWFRLAAAQGNVPAQYSLGLAYEKG
                     QGVPQDYQEAVK"
BASE COUNT          888 a         1106 c         1098 g          820 t
ORIGIN      
        1 gaccttaaag aaggcaatcg atgccgccgg catcagtgaa gcgttcgtca gcgccatcgc
       61 cccgggcagc ctggaaatgt tctgccgcga tcagaacagt tattacccga caattgagaa
      121 attcttagaa gggatcgccg cggcgctgcg cgaggaatat cgtgcgatca tcgacgccgg
      181 gttcatcttg caactcgacg atcctggctt gcccggcgcc tgggacatgc tcgatccgca
      241 gccgagcgtc gaggagtatc gacgctacgc atcgctgcgc gtcgagatat tgaaccacgc
      301 actccaaggc atccccatag agcgggtgcg ctatcacatc tgctggggca gctggcatgg
      361 tccgcacacc actgattttc cgttgcgcga catcgtcgat atcatgctcc gggtcaacgc
      421 ccaggcttac ttgctcgaag ccggcaacgt ccgccacgaa catgaataca aagtctggcg
      481 agacgtaaaa ttgccggccg gaaaaattct cattcccgga gtcgtcagcc actccaccga
      541 tctcgtcgag caccccgaac tggtggccga ccgcatcctc gccttcgccg acatcgtcgg
      601 ccgcgaaaac gtcattgccg gcaccgattg cggcctgggc gggcgcgttc atccacaaat
      661 cgcctgggcc aaactgcgcg tgctgagcga aggcgcggcg atcgcaagca agaaactgtg
      721 gcgttgagat tcaaaagtat tcggaggatt gttaggattg agcgatttga ctgcagatac
      781 ttcgccgctc gccattgctg gatttgcaat ggtcaatcaa gcgaacttga tgagtgcaat
      841 ttggcaaccc ccgcaatttt gtgcccgtct taagaggcgg ggattatagg aatcaaacta
      901 acgaggagac ctgaatggaa attactcttt actacgcccc ccatacctgc gcggtcgctc
      961 cctatatcac actgaccgaa gcgggcgccg atttcgaggt ccggccgctc aattttcgca
     1021 agcggcagaa ttacacgccc gagtatctaa aaataaaccc aaagcacaag gtgcctgcgc
     1081 tcgtgatcga cggcaagata ttgaccgaga acgtcgccat ccatcaatgg gttcatcgaa
     1141 ctttccccga cgcgaagatt ctgcccagcg attcgtggga ccagctgcag gcgatctcgc
     1201 tgcacgcctg gtgtgccagc ggcattcacc cctatctgag ccggatcaac aatccgccca
     1261 aagtatgcga cgccaccggc gcgtccgaca gcgtgatcaa gttcgccact gaaattctgt
     1321 tcgagaactt ccagatcggc gacgatttgc tcgccgggcg cgagtacttc ttcgatcact
     1381 tcaccgcccc ggacgcgcat ttcttttggt gctgccgccg cgctacccag ctggaagtgg
     1441 acatctccgg tttccccaat gtcgtcgagc atttcaagcg catgcagcgg cgcccgagcg
     1501 tcaagaagct cctcgcctac gagaaacaag tgaatgaaga gtttgccaag acggcctagc
     1561 atattaatga agagtccgga ttggaggctg ctcaaaaaga cctccgagcc gaggcgcgcg
     1621 aaaaaacggg gagcgcctag cgagtacgtc gtagcgagac gattgagcgc aaaccaagga
     1681 tatgagtctt tttcagcacc gtgcgaagag ggaatcagcg agagacgacg aaccaagcag
     1741 cccatttgca tttcaacacg gtcagcaaat ttttcgagct attattagtt taagattcga
     1801 ctttgagtca ttctcgggag taacgtgcca gaaaccgcaa ccgtgatcgg ccgctcaggc
     1861 ttccgcattt cagctcttat gttgattgtg ctcatggcgc attcggtcgg ctgtgcgcgc
     1921 cggtttccgg tgactgaagt cacgacgata aaggccgcaa accccgccga gcttcaacga
     1981 catcttctgt cgcgcaagcc cgacgtagcg cagttcaagt tgcgcgggcc ctttgacgtc
     2041 accgagcgca gggacttgga aatccgcctg gcttccggtc tcacggtggc agcggactta
     2101 tatttatgcg cagcggccca caaggctcct ctggtcatcg tgttgcacgg tctcgacaat
     2161 tccaaggagg accatgcctt ccaagcgatg cacttggcct cctggggcat gcatagtctg
     2221 gctctcgact tgcccaaaca gggtccatgg atcaccaacg gcagaaccct tgccaggctg
     2281 gtcgacgcca ttcgcaaaac gccgcagctc gtcgacggcc gcatcgaagc ggaaaaggtc
     2341 attctcgtgg gccactcttt cggcgcaacc gccgtcgcca ccgcgttggg ggcaggcgcc
     2401 cccgcgctcg ggggcgtgct tcttgatccc gccgggatcg gccgggaatt gccgcagttg
     2461 ctgaggcgga tcaccgttcc ggtgatggtg atcggtgccg acgaggacat ttggccgacc
     2521 cgcaaccgcg gacaatttta ccggtttatt ccggccgctg tcggagagat ttcgatacgg
     2581 gatacggtcc acgaagatgc gcagtatccg aaccaccaca cactgcgccc attcgaagac
     2641 gaaccggatg acactgagga ggcgcagatc actttcgtca gcgcactgac cgcctccgcc
     2701 ttcggtcttg cggccaccgc cagcatagac tatggctgga acagctttga actagccatt
     2761 aagaacggca cgtttttcaa cgcgcggagg aagtaatcgt catgcgttca gtcaaatctt
     2821 tcttaaaaag gtggaagacc gcggaaacag gcgtagccgt tttgatgatc ctgcgagcgc
     2881 ttggtccggc agccgtctgt cccggttcgt tgacaaggtc ctgagtttgc tggaggtcgg
     2941 cggcgtcttc tacacgctgg tgtcaggggt gcacttagag ggcggcgata aactcggcag
     3001 ctggtatctg accgagttgg aaaattccgc cggccggccc gagaaagtct gctcctggct
     3061 gaaaaagtgc acctgcgtta aagtcacttg cgagtcgaag agcgactgga aaaggcccac
     3121 cgagctgata aaagtcgaca aggtttgcaa cgacgtctct gtcccgcgca tggaattatt
     3181 ggagttcgtc gcgggatatc ctccaaacag gcgattcaaa tggcacccat agggtgataa
     3241 attttgcttt acatttgcga accgggtcga tcactgacgc gtaaaattgg ctggttttca
     3301 ggctttccca gcggcgcggc gcatttcaag cccatgcaga agattccgtg ttttaagaag
     3361 ctgcttgcct atggaagata agtaaacggc gggacttgtc atgtcggcca aagcgacttt
     3421 cgactcttgc cgactcactc cgttgcccgg atgcataaag agcaagttcg aaaattaaaa
     3481 attttacgat tcaagaaatc acgacagtca acagcggact catgcaacag tcaggcaacc
     3541 cactgtcggt aactgcatgg gaatcgccat gaaatttaaa ctgattttgc tcgcctttat
     3601 ttatttgctc ggtaccagta gttttgccgc atcaaaccct tttgatgacg caagcgccgc
     3661 ctacgagcgc ggcgactatg tccatgcgat tcacctcttt cgcgagctgg ccgcgaaggg
     3721 acaccaatgg gcgcagagga ggctgggttc gatatacgca caggggcatg gcgtgccgca
     3781 ggattatcag gaggcggtaa agtggtttcg cctcgcggca gcgcagggaa atgtcccggc
     3841 gcagtatagt ctcggtctgg cttatgagaa agggcaaggc gtgccgcagg attatcagga
     3901 agcggtgaag tg
//
LOCUS       DASQKS010000321        11086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5619265, whole genome shotgun sequence.
ACCESSION   DASQKS010000321 DASQKS010000000
VERSION     DASQKS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5619265"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VE170_08385"
     CDS             <1..847
                     /locus_tag="VE170_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYJ15493.1"
                     /translation="KWFAGALLAGALVWSIGGTVAAAEKRPIDVIEGDSFLIEEAYNQ
                     EPGVVQHIFTAAYTKSGRTQGWAFNFTREWPVFSQDHQLSYTIPSFHMRDDGSRVSGL
                     GDILLNYRYQLVDEADAKPAVAPRFSLILPTGNRKKGTGNNVVGYQLSLPVSKKISNT
                     VALHGNAGATFLPHVKAPVDINGNSAKKSLTAANIGGSAIYALLPRFHLMLEWVGNFD
                     QSFSDAGKRERAFLATMSPGFRTAIVDQDKLQIVTGAAVPIGLNRKTDNLGAFLYLSI
                     EHDLF"
     gene            880..1971
                     /locus_tag="VE170_08390"
     CDS             880..1971
                     /locus_tag="VE170_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15494.1"
                     /translation="MNGIIDADTHISEGAAMWALMDRAMQPRRPIMMQVPDDTWYGNR
                     DAFWLIDGEIYPKPAGRGSFALVTPSEQKVQAGRKDSTPATREMTDIKGRLADMVKLD
                     TAMQIVYPTLFLVYNTKDKELEVALCRAYNRFLSQACAQSGGLMKWVVVLPLQSMNVA
                     IDEIRFAKANGAVGVFFRGIEGEYTLDNPYLFPVYEEAQKHNLSICVHTGCGVKQVLE
                     MFDLARNHTFGHTRVMPLLAFRDLIANKIPEQFPGLRFGFIEAAAGWAPFLIHIIRRL
                     LKERFKYGSSAEMFREYRLYIACEADEDIPYIAKYTGDDHLLIGSDYPHNDPSREDQF
                     VNALNVREDISPQLRQKILYDNPRAFYAL"
     gene            2080..3039
                     /locus_tag="VE170_08395"
     CDS             2080..3039
                     /locus_tag="VE170_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15495.1"
                     /translation="MKLHRVFKFSFVTLALLWGVPAVGQEVFIGNPGKSLNFFHFDLA
                     IERGYFRELGLDVKLLHTKCDVAVTALLTGDLQASGCVGTASRVIASQNVPVRTVIWL
                     FKKPTFYVVARPEIKSAADLRGKIIGISSFGSDTDLAMKVFAQSGKLDPDKDIKRIVA
                     GSTATRLQGLKAGSMDATTLSPPFNIYAEQLGLRVLAYTGDYLEFPQSGFTVSDATLK
                     NKRDLIKRLLRGTLRGLQFTLKNRADTVRFIAKDYKLQEAVAEKVYTSLLPAMSENGL
                     ATDKGLRNMIETLGTAVGKNVDFRPERLVDYNLLREVQKELGM"
     gene            3056..4006
                     /locus_tag="VE170_08400"
     CDS             3056..4006
                     /locus_tag="VE170_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15496.1"
                     /translation="MNLIFQKPFLGLLGLALVASGAFAADAPQKIRIVYASRSSSALP
                     QYMAVQKGYFKEQALDVEIIQMNPRLGAAAVTSGEVAFATPFTSTFRGALQGLPIKLV
                     FVHIKKGPYYVMVRPEIKDVQQLRGKKIGVATIKGTDQLVAEEMLQAKGFNISQIQAV
                     AIGDGPVRMQALVSGAVEAVCLAPPHDFMLRKMGYNALLGPPDVGLPSAGMLTSDKLI
                     KENPQLVRRTLKALLRSHHHIIENRQDAIQTLIKWLPQPIDIAEHSYDAELRTGLNRD
                     GTMTDAEIEAIIDRVGEKKRPLDEVRDFSFARAALKELGR"
     gene            complement(4102..5241)
                     /locus_tag="VE170_08405"
     CDS             complement(4102..5241)
                     /locus_tag="VE170_08405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ15497.1"
                     /translation="MDFGFTQEQQHLIERVNALVKERIAPRAAQYDAEFAMPVEDIQD
                     LHRQGWLLANLDKRRGGLGFGLHGDDPLSFFLIDEHLAYGNPSTAHCFQVHNNGLMMI
                     DAMASDAQVKQWLAPTIERGALLPGAGAEPYGVAPTSAKKTSGGYLVSGTKHYATNAS
                     MAEWLWIGRVASDSHPKPIMFMLHKDTPGLKIDTEAWRPTGMRACVSPWLYLENCFVP
                     EENLLGQPGQFLGENWMGKINFAFTANYLGSARAMYDWALAYIRERNGARDSYRQLRF
                     GELKSMLDAARLILYNAVRMFKNNPNEAMIAAHEAKWMAKETLEKVIWSSAEICGSTA
                     LFVKHPLERFYRDMHLHMMHGRHDIAAQIVGASELGEEFDTNRNH"
     gene            complement(5575..6561)
                     /locus_tag="VE170_08410"
     CDS             complement(5575..6561)
                     /locus_tag="VE170_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15498.1"
                     /translation="MRSGGSRYLFLAVLIAALSANSVVRSAELMPLRATYSSIGGAFA
                     PLWLALDKGLFAKYGLAVDMKYLLSATGTQALLAGSVDIVNPATEIIEAGLGGARVAF
                     IIGILNRAVLSIYSKPEFKQLSDLRGKVFGVTLPGSTTDVTAKILFQQAGMVTGKDVQ
                     ITHLQGMPDIITALGAGRIDAAVVSAPSTLKLRQAGLKELVDIGAKNVPLIHAGLATT
                     RDFIKANPDKVRRYVQAYIEGTKIARTDPEAAKQVIAKYTKTDNKEDLDETYNTYVKF
                     WEQAPYVSAAGMQTMLNFSVNPAGKTAKPEQFIDNSFVGELEKSGFVKELYK"
     gene            6711..7667
                     /locus_tag="VE170_08415"
     CDS             6711..7667
                     /locus_tag="VE170_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15499.1"
                     /translation="MAGILLRVFGVILTGSMLAAMPARGADAPQRIRFAYASRSNIVA
                     PKHIAQSRGYFRAEGLDVEMIQMNPRLSATAIINGDVTYADAFTSTFRGIMQGFPIKL
                     VLVHQKKGPYFLIARPEFKDIQQLKGKKLGVATLRGSDHLVADELMLSKGFNPATVQP
                     IVIGDASMRYQAMIGGIIDFVAVATPQDLMLRQKGFTVFAGPPEVGVPGSGVFTSEKF
                     LRENSPAVKKTLRALLRAHHYILDNRSETIQAMMQWLPQSLEVASHSYDIEMKALTPD
                     GLMTDGEIDTLVAKLGEKKRPMDEIRDFTLLRQALKELESGK"
     gene            7719..8669
                     /locus_tag="VE170_08420"
     CDS             7719..8669
                     /locus_tag="VE170_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15500.1"
                     /translation="MGKIAAIGLTIAMLASAPGDLRAAEAPQKVRFLHASRSNSMAPK
                     LIAQSKGFFGAEGLETEMIQMNPRLAAVALLSGEAAFSDAFTSVFRGILQGFPIKTVF
                     VHQKKGPYFLLARPEFKEVQQLKGKKLGVATLRGSDHLVAEELLQSKGFNPSLIQVLA
                     VGDAATRFQSLSSGAVDVIAVGTPHDLMLRQKGFSVLAGPPEVGLPGACVFTPDRFLR
                     EHPQTVRKTLKALLRAHRYILDNRQETIQALLQWLPQTPEVAAHSYDFELKTMTKDGL
                     MSDAELEAVMIKLGEKKRPLDEVRDFSLARQAMKELEAGK"
     gene            complement(8753..9160)
                     /locus_tag="VE170_08425"
     CDS             complement(8753..9160)
                     /locus_tag="VE170_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15501.1"
                     /translation="MAQELKEFRHKTPEFEGPKKTWQVCNSDLMKVQVQVVKDGGENN
                     LHTHTGEDAFWYVVSGAVRFYGEGDKVIGEYQKGEGVLVPRGFKYWFESASSETLEVL
                     RVTAKDQNIDNKRVDLSPQKDWMIQQNTFGARQ"
     gene            9302..10285
                     /locus_tag="VE170_08430"
     CDS             9302..10285
                     /locus_tag="VE170_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15502.1"
                     /translation="MTRCKISLLVVSLFWLWPALAAAQNLPLLNMGYSGAGIGSDLLK
                     VIDRAGLWRRHGIEVRTIYLSSGSLMAQTMSSGDIGVAGFDTPAMLNLAVAGTNLKVV
                     AVPINRIEPMFVVRSNIKVPGDLRGKRVTISRFGSGSDIMTRVALRHWKLDPEKDVSF
                     LQTGNTPTRIAALVAGQVDAALVSSTGVAKIIATGCCRVLADLAELPIEFANYGVMVS
                     GSLLKNQRDPIKRFLMAVIEGIHVFKTKPEIARGVLRESNSDADAIGPLYERLSKSFR
                     DFPAPEPRGIQNVLDSLMVPKAKGARAEDFMDTTLMDEIRKSGFVERLTGK"
     gene            10301..>11086
                     /locus_tag="VE170_08435"
     CDS             10301..>11086
                     /locus_tag="VE170_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15503.1"
                     /translation="MGNLIVANALIVAMTERASFHGYVRVRDGVITEVGAGAPRQTTT
                     KEEEAIDALGCVLMPGLVNAHTHLYQVLLRAVWEDLELMPWLKRIYGCARVLTPAHFY
                     AGSLLGCVESIRGGVTTVCEHNFLNPSPECALETIRAMQDAGVRSVFARTIMDSGEIV
                     PECTKEKPEQAFRLIEALLARHKNSRQLTFMTGPNTPPINTTPDLLREVRRFADAHSL
                     GISAHVAESKSVVEAVRRQHGMNGVVELLHRFGIPAKNSIFAHS"
BASE COUNT         2311 a         3036 c         3324 g         2415 t
ORIGIN      
        1 caaatggttt gccggcgcat tgctggcggg ggcgctggtg tggtcgattg gcggcacagt
       61 tgcagccgcg gaaaaacgac ccatcgacgt gatcgaaggc gatagttttc ttatcgagga
      121 ggcgtataac caggagcccg gcgtggtgca gcatattttc accgcggcgt acactaaatc
      181 gggcaggacc cagggatggg cattcaactt cacccgggag tggccggtat tttcccagga
      241 tcaccagctg tcttacacga tcccgtcatt tcatatgcgc gatgacggct cgcgggtatc
      301 cggcttgggc gatatcctgc ttaactatcg ctaccagctg gtcgacgaag cggatgccaa
      361 acctgcggtg gcgccgcgct tcagtttgat cctgccgacc ggtaatcgca aaaaaggcac
      421 gggcaacaac gtcgtcggtt atcagttgag tttgccggtc agcaaaaaaa tttccaatac
      481 cgtggcactc catggcaacg ccggcgcgac ttttcttccg catgtcaaag cgccggtgga
      541 tattaacggc aactcggcaa aaaaatctct gaccgccgcc aatatcggcg gcagcgcgat
      601 ctacgcgttg ttgccgcggt ttcatttgat gctggagtgg gtcggcaact tcgatcagag
      661 ctttagcgat gcgggcaagc gtgagcgcgc gttcctggcg acgatgtcgc cgggtttccg
      721 gacggcgatc gtcgatcaag acaagctgca gatcgtcacc ggcgcggcgg tgccgatcgg
      781 gctcaatcgt aagaccgata acctcggcgc gtttctttat ctatcgatcg agcacgatct
      841 gttctaaagg gattggcaaa aacacactcg ggggtaccta tgaatggaat tatcgacgct
      901 gacacgcata tttccgaagg ggcggcgatg tgggcgctga tggaccgggc gatgcagccg
      961 cgtcggccga ttatgatgca agtgccggac gacacttggt acggcaatcg tgacgcattc
     1021 tggctcatcg acggcgagat ctatcccaag ccggcaggca ggggaagttt cgcgttggtc
     1081 acgccgtcgg aacaaaaagt tcaggccgga cgcaaagact cgacaccggc gacgcgcgag
     1141 atgaccgaca tcaagggccg gctggccgac atggtcaagc tcgacacggc gatgcagatc
     1201 gtctatccga ccttgtttct agtctacaac accaaggaca aagagctgga agtggcgctt
     1261 tgccgcgctt acaaccgctt tctctcacag gcctgtgcgc aatccggcgg cctgatgaaa
     1321 tgggtggtgg tgttgccgct gcaatcgatg aatgttgcca tcgatgaaat ccgcttcgcc
     1381 aaagcgaacg gcgcagtggg agtattcttc cgcggcatcg aaggcgaata tacactggac
     1441 aatccctatc tgtttccggt ctacgaagag gcgcagaagc ataacctgtc gatctgcgtg
     1501 cataccggct gcggcgtgaa gcaagtcttg gagatgttcg atctggcgcg caaccacact
     1561 tttggccata cccgggtgat gccgctcttg gcctttcgcg atctaatcgc caataaaatc
     1621 cccgagcagt ttcccggcct ccgcttcggc ttcatcgaag cggccgcggg ttgggcgccg
     1681 tttttgattc acatcattcg gcgcctgctc aaagaacgct tcaaatacgg cagcagcgcc
     1741 gagatgttcc gtgaataccg gctttatatc gcctgcgaag ccgacgaaga tattccctat
     1801 atcgccaagt acacgggcga tgatcacctg ctgatcggtt cggactatcc gcacaacgat
     1861 ccgtcccgtg aagaccagtt cgtcaacgcg ctcaatgtcc gcgaagacat ctcgccgcaa
     1921 ttgcggcaga aaatcttgta cgacaatccg cgcgcgtttt atgcgctcta gaagaaatgg
     1981 agcgttggag tgctggaggg gtggaatgat gggtttccga cccaatactc caatattcca
     2041 gaactccatc actccgggcg gcgggcggga aaaacgacaa tgaaattgca tcgcgtcttt
     2101 aaatttagtt ttgtcacgct cgctttgctg tggggcgtac cggcggtggg gcaggaagtt
     2161 ttcatcggca atccgggcaa gagccttaac ttttttcatt tcgacctggc gatcgagcgc
     2221 ggctattttc gcgagcttgg tctcgacgtc aaattgcttc acaccaagtg cgacgtcgcg
     2281 gtgacggcgt tgctcaccgg cgacttgcaa gcgagcggct gcgtcggcac cgcgtcgcgg
     2341 gtgattgcca gccagaacgt gccggtgcgc acggtgatct ggctattcaa gaaaccaact
     2401 ttttacgtcg tcgccaggcc ggaaatcaaa agtgccgccg acctgcgcgg caagatcatc
     2461 ggcatcagct cttttggcag cgacaccgat ctggcgatga aagtttttgc ccagagcggt
     2521 aagctcgacc cggataaaga tatcaagcgc atcgtcgccg gctcgacggc gacccgcttg
     2581 cagggattga aagcgggatc gatggacgca acgacattgt cgccgccgtt caatatctac
     2641 gccgagcagt tggggttgcg cgtactggcc tacactgggg attatctgga attccctcag
     2701 agcggtttca ccgtttccga tgcgactctc aaaaacaagc gcgatttgat caaacggctg
     2761 ctgcgcggca ccttgcgcgg tttgcagttc acgctgaaaa atcgcgccga tacggtgcgt
     2821 ttcatcgcca aggattacaa gctgcaagaa gcggtggccg aaaaagtgta tacctcgctc
     2881 ctgccggcca tgagcgaaaa cggtttggcg actgacaagg gactcaggaa catgatcgaa
     2941 accctcggca cggcagtggg caagaacgtc gactttcggc cggagcggtt ggtcgactac
     3001 aatttactga gagaagtgca aaaagagttg ggcatgtaac gaaaggaaag aggcgatgaa
     3061 tttgattttt cagaagccgt tcttaggatt gctgggactc gcgctggttg ccagtggcgc
     3121 attcgccgcc gacgcgccgc agaaaattcg catcgtttac gctagccgca gcagctcggc
     3181 gctaccccaa tacatggctg tgcaaaaagg ttacttcaaa gagcaggccc tcgatgtcga
     3241 aatcattcag atgaacccgc gcctgggtgc cgcagctgtc accagcggcg aggtcgcctt
     3301 cgcgacgccg ttcaccagca cttttcgcgg tgctctccag ggcctgccga ttaagcttgt
     3361 tttcgtccac attaagaaag gtccctacta tgtgatggtg cgccccgaga tcaaagacgt
     3421 tcaacaactc cgaggtaaga agatcggtgt cgcgacgatc aagggcacgg accagttggt
     3481 ggccgaggag atgctacaag ctaaaggatt caacattagc cagattcaag ccgttgccat
     3541 cggcgatggg ccggtgcgca tgcaggcgct agttagcggc gccgtcgaag cggtgtgcct
     3601 ggcgccgccc catgatttca tgctgcgcaa gatgggctac aacgcgctcc tcggtccgcc
     3661 ggacgtcggt ctgccttccg ccggaatgct gacgtcagac aagctgatca aagaaaatcc
     3721 ccaactggtg cggcgcacgc taaaggcgct gctgcgctcg caccatcata ttatcgagaa
     3781 tcgccaagat gcgatccaga cgctgatcaa atggctgccg caaccgatcg acatcgccga
     3841 gcactcttac gacgccgagc taaggacggg gctcaaccgc gacggcacca tgaccgacgc
     3901 cgagatcgaa gcgatcatcg atcgggtcgg tgaaaagaag cggccgctgg acgaagtgag
     3961 agatttttcc ttcgcgcgcg cggcgctgaa ggagctggga cggtgaggat tggagtaatg
     4021 gagtgttgga gagttggagt gatgggtttc ggatttccgg accaatactc cactactcca
     4081 gcactccact ctcccattct tctagtggtt tcgattggtg tcgaactctt cgcctaactc
     4141 agacgcgccg acgatctgcg cggcgatgtc gtggcggcca tgcatcatgt gcaggtgcat
     4201 gtcgcggtaa aaccgttcca gcggatgttt gacgaatagc gccgtcgagc cgcagatttc
     4261 cgcgctgctc cagataactt tttcgagagt ttccttagcc atccacttgg cttcatgggc
     4321 ggcgatcatc gcctcgttgg gattgttctt gaacatgcgc acggcgttgt agagaatcag
     4381 ccgagcggcg tcgagcatgc ttttgagctc gccgaagcgt agttgccggt agctgtccct
     4441 ggcgccgttt ctttcgcgaa tataggcgag cgcccagtcg tacatggcgc gcgctgagcc
     4501 taaatagttc gcggtaaagg cgaagttgat tttgcccatc cagttttcgc cgagaaattg
     4561 cccgggctgc cccaacaaat tttcttcggg cacgaagcag ttttccaagt acaaccatgg
     4621 gctgacgcag gcgcgcatgc cggtggggcg ccacgcctcg gtgtcgattt tcaagcccgg
     4681 cgtgtctttg tgcagcatga acatgatcgg cttgggatgg gaatccgacg ccacccggcc
     4741 gatccagagc cactcggcca tgctcgcatt ggtggcgtag tgtttggtgc cgctgacgag
     4801 ataaccgccc gacgtttttt tcgcgctggt cggcgccacg ccgtagggtt cggcgccggc
     4861 gccggggagc agcgcgccgc gctcgatggt cggtgccagc cattgtttca cctgcgcatc
     4921 gcttgccatg gcgtcgatca tcatcaggcc gttgttgtgc acctgaaagc agtgcgccgt
     4981 ggatggattg ccgtaggcga ggtgctcgtc gatcaagaaa aaactcagtg gatcgtcgcc
     5041 gtgcagaccg aagcccaagc cgccgcggcg tttgtcgaga ttggcaagca gccagccctg
     5101 gcgatgcaga tcctggatgt cttccaccgg catggcgaac tcggcgtcgt actgcgccgc
     5161 gcgcggggca atgcgttcct tgacgagtgc gttaacgcgc tcgatcaggt gctgctgttc
     5221 ttgagtgaag ccgaagtcca atggctgcct ttctgattgc taacctgaag gggtctgttc
     5281 accatgaagg gcacgaagtt cggaagatcg ttaacttcgt tccttcgtgc ttcgtgttcg
     5341 cctaagaaaa taaatgttag gggagagtct ccacaatgaa gaggtcccgt ggactccgtc
     5401 ataccagcga aagcgggtat ccaggctaat tgggcgcgca gaccacctgg attcccgcgt
     5461 gcgcgggaat gacgcctcaa gcagaccgcc tatggggaat cccatccata ttaatattct
     5521 gtagacgagc gtaagctcat ggacaatacg cgctgaatga aaatttgtta ccggttattt
     5581 gtagagttct ttgacgaagc cggacttttc aagctcgccg acgaaggagt tgtcgatgaa
     5641 ctgttccggc ttggcggttt tgccggcggg attgaccgaa aaattaagca tcgtctgcat
     5701 cccggcagcg gaaacgtagg gggcctgctc ccaaaatttc acataggtgt tgtaggtttc
     5761 gtcgaggtct tccttgttat cagtcttggt gtatttggcg atgacttgtt ttgccgcctc
     5821 gggatcggta cgagcgattt tcgtgccttc aatataggcc tgtacgtagc gccgcacttt
     5881 gtcgggattg gccttgatga agtcgcgcgt ggtcgccagt ccggcgtgga tcagcgggac
     5941 atttttcgcg ccgatgtcga ccaattcttt gagaccggct tggcgcagct tcaacgttga
     6001 cggcgcggac accaccgcgg cgtcgatgcg ccccgcgccg agcgcggtaa tgatatcggg
     6061 catgccttgc aggtgcgtga tctgcacgtc tttgcccgta accatgccgg cctgctgaaa
     6121 cagaatctta gcggtgacgt cggtggtcga gccgggtagg gtcacgccga agactttgcc
     6181 gcgcaggtcg gagagctgtt tgaactccgg tttgctgtag attgacagca cggcgcgatt
     6241 caatatgccg atgatgaagg cgactcgcgc gccgcccaat cccgcttcga tgatttccgt
     6301 cgccgggttg acgatgtcga cgctgccggc taacagcgcc tgggtgccgg tggccgagag
     6361 caagtatttc atgtctaccg ccaggccgta ttttgcaaat agccctttgt cgagggcgag
     6421 ccaaagcgga gcaaaggcgc caccgatcga cgagtaggtc gcccgcagcg gcatcagctc
     6481 ggcgctgcga accacgctgt tcgccgatag ggctgctatc agtacggcta agaaaaggta
     6541 acggctacca ccagaacgca tcggacttcc ccccggaaaa aaagtttagg cagatatatt
     6601 aaaaaatggc gcgttgcgct aggggcggcg ataacatggg tttaacgcta tctcttacgg
     6661 tgctgcttat gctaggaagg attcggtaat cgaacccaag gagaagatcc atggccggta
     6721 ttttattgcg ggtttttggt gtgatcctga cagggtcgat gttggcggcc atgccggcac
     6781 ggggggcgga tgcgcctcaa cggattcgct ttgcctacgc tagccggagc aacatcgtcg
     6841 ccccgaaaca tatcgcccag tcgcgcggtt actttcgcgc cgaaggtctc gacgtcgaga
     6901 tgatccagat gaatccgcgt ctgagcgcaa ccgcgatcat caacggcgac gtcacctatg
     6961 ccgatgcgtt taccagcacg tttcgcggca tcatgcaggg atttccgatc aaattggttc
     7021 tggtgcacca aaagaagggg ccgtatttcc tgattgcccg tccggagttc aaagacattc
     7081 aacagttgaa gggcaaaaag ctcggggtcg cgacattgcg cggctcggat cacctggtcg
     7141 ccgacgagtt gatgctgtcg aaaggattca atccggcgac cgtgcagccg atcgtaatcg
     7201 gcgacgcgtc gatgcgctat caggcgatga tcggcggtat catcgatttt gtcgcggtgg
     7261 cgacacccca ggacttgatg ttgcgccaga agggcttcac cgtcttcgcc gggccgccgg
     7321 aagtcggcgt gccgggttcg ggggtgttca cctcggaaaa gtttctccgg gaaaattccc
     7381 cggcggtgaa aaaaaccttg cgcgcgctgc tgcgggcgca ccattacatt ctcgacaacc
     7441 gtagcgaaac gattcaagcg atgatgcagt ggctgccgca atcgttggag gtggccagcc
     7501 actcctatga tatcgagatg aaggcgttga cgccggatgg cttgatgacc gacggcgaga
     7561 tcgacacgct ggttgccaag ctcggcgaga aaaaacggcc gatggacgag atccgagact
     7621 ttacgctgct taggcaagcg ctcaaggaat tggagagcgg taaataggca agagacggcg
     7681 ggcaccgtct tctctagcgc agcgagggat cggcgacaat gggaaaaatc gcagcgattg
     7741 gattgacgat cgccatgcta gcttcggcgc ccggcgacct tcgcgccgct gaagctccgc
     7801 aaaaagttcg ctttcttcac gccagccgaa gtaactcgat ggcgcccaag ctgatcgccc
     7861 agtccaaagg tttctttgga gccgaagggc tggagaccga gatgattcag atgaatccgc
     7921 gcttggccgc tgtcgcgctc ctgagcggcg aagcggcgtt ttcggacgcg tttaccagcg
     7981 tgttccgcgg tatcttgcag ggtttcccaa tcaagaccgt ctttgtgcac cagaaaaagg
     8041 ggccgtattt tttactggcg cggccggagt tcaaagaggt ccagcagttg aagggcaaga
     8101 agctcggtgt ggcgaccttg cggggttccg atcatttggt ggcggaggag ttactccaat
     8161 cgaagggctt caacccaagc ttgattcagg tgctcgccgt cggcgatgcg gcgacgcgct
     8221 ttcaatccct cagcagtggc gccgtcgatg tgatcgcggt gggcacgccc catgatctga
     8281 tgctgcggca aaaagggttc tccgtgctcg ccgggccgcc ggaagtcggt ttgcccggcg
     8341 cctgtgtatt cacgcccgac cggtttctgc gcgagcatcc gcagaccgtg agaaagactt
     8401 tgaaagcgtt gttgcgtgcc catcgataca ttctcgacaa ccgccaagag actattcaag
     8461 cgctactgca atggctgcct cagactccgg aagtggcggc ccattcctat gatttcgaac
     8521 tgaaaacgat gaccaaagac ggtttgatga gcgatgccga actcgaagct gtgatgatca
     8581 agttgggcga gaagaagcgg cccctagatg aagtgcggga cttttccctg gcgcgccagg
     8641 ccatgaaaga attggaagcg gggaaatagg aaataaggag tgaatagtaa ggagtaagga
     8701 gtctcgtatc agaacccctt cctcctcact ctttaatcct tccagtttta gattactgcc
     8761 gggcgccgaa agtgttctgt tgaatcatcc aatctttttg cggcgacagg tcgactcgct
     8821 tgttgtcgat gttctggtcc ttggcggtga cgcggagtac ttccagcgtt tcgctcgaag
     8881 cgctttcgaa ccagtactta aagccgcgcg gcaccaagac gccttcgcct ttttgatact
     8941 cgccgatgac cttgtcgcct tcgccgtaga agcgcaccgc gccgctgacg acgtaccaga
     9001 aggcatcctc gccggtatgg gtatgcagat tattctcgcc accgtctttg acaacctgga
     9061 cttgcacctt catcaggtca ctattgcaaa cctgccaggt tttttttggg ccctcgaact
     9121 cgggggtctt atgtcgaaat tcttttaatt cctgtgccat gggtcacctc gtcatgaaaa
     9181 ttttccccac cttaatcccg attggggacg gcggtcaagc gtggcggcgc ttgaaaataa
     9241 tcgcgagccg ttgacggtgt attttcggta tattacttat gccgtcactg aggtgaatcc
     9301 gatgacgcga tgcaaaattt cgctgctggt ggtttctctg ttctggctgt ggccggcctt
     9361 agccgcggcg cagaatctac cgttgctcaa catgggttat agcggcgccg gcataggctc
     9421 ggatttgctg aaagtgatcg accgcgccgg tttgtggcgc aggcatggca tcgaggtgcg
     9481 gacgatctac ctgtcgagcg gttctttgat ggcgcagacc atgtcgtcgg gcgatatcgg
     9541 cgtcgccggc ttcgacactc cggcgatgct caacctggcg gtggcaggaa ccaacctcaa
     9601 ggtggtcgcc gtgccgatca atcgtatcga gccgatgttc gtggtgcgca gcaatattaa
     9661 agtacccggc gatttgaggg gcaagcgcgt gaccatcagc cgcttcggtt cgggatccga
     9721 tatcatgact cgggtcgcgc tgcgccattg gaagcttgat cccgagaagg acgtgagctt
     9781 tcttcagacc ggcaatacgc caacgcgcat cgccgccctg gtggccgggc aggtggatgc
     9841 ggcattggta agctccaccg gcgtggccaa gatcatcgcc accggctgtt gccgggtgct
     9901 ggccgatctt gccgaactgc ccatcgagtt tgccaactac ggcgttatgg tctcgggctc
     9961 gttgctcaag aatcagcgcg atcctatcaa acgtttcctc atggcggtga tcgaagggat
    10021 tcatgtgttc aagaccaagc cggagatcgc acgcggggta ttacgcgaga gtaacagcga
    10081 tgccgatgcg atcggcccgc tctacgaacg gctgagcaaa tcctttcgtg actttcccgc
    10141 tcccgagccg cgcggcatcc agaatgtcct cgattcgttg atggtgccca aggcaaaggg
    10201 cgcccgcgcc gaagatttca tggatacgac cctaatggac gagatccgca agagcggctt
    10261 tgtcgagcgc ctgaccggga agtgagatcg agacggtacg atgggtaatc tgatcgttgc
    10321 caatgcgctg atcgtcgcta tgaccgagcg cgcctcgttc cacggctatg tgcgcgtgcg
    10381 tgacggtgtg attaccgagg tgggtgccgg cgcgccgcgg caaacaacga ccaaggagga
    10441 agaggctatc gatgccttag gctgcgttct catgcctggt ctggtcaacg cccacaccca
    10501 cctctatcaa gtcttgctgc gcgcggtgtg ggaagatttg gaattgatgc cttggttgaa
    10561 aaggatctac ggctgcgccc gggtgctgac gccggcgcat ttttacgccg gcagcttgct
    10621 cggttgtgtc gaatctatcc gcggcggcgt gaccacggtg tgcgaacata attttcttaa
    10681 tcccagcccg gaatgtgccc tggaaacaat ccgggcgatg caggatgccg gtgtacgttc
    10741 ggtgtttgcc cgcacgatta tggatagcgg ggagatcgtg ccggagtgta ccaaggagaa
    10801 gccggaacag gcgtttcgtc tcatcgaagc gctgctcgca cggcacaaaa attctcggca
    10861 gctcactttc atgaccgggc cgaacacgcc gccgatcaat acgacgcccg acctgcttcg
    10921 cgaagtgcgc cgatttgccg atgcccattc cctcggcatc agcgcccatg tcgccgagtc
    10981 gaagtcggtg gtcgaagcgg tgcgccggca gcatggcatg aacggcgtcg tcgaattgtt
    11041 gcatcggttc ggcattcccg cgaagaattc gatcttcgcc cactcg
//
LOCUS       DASQKS010000322         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3944415, whole genome shotgun sequence.
ACCESSION   DASQKS010000322 DASQKS010000000
VERSION     DASQKS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3944415"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VE170_08440"
     CDS             complement(<1..808)
                     /locus_tag="VE170_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ15504.1"
                     /translation="MRASDIFRLVLLAAIWGGSFVFLRVVAPVFGPIVTADLRVLIAW
                     GALAIYCRTVGLNVGWRRFWKQYLIIGAFNAALPFALFAFAALHIPASYSAILNATSP
                     FFGALCAGLWLGEKITLPKCLGFVLGIAGVALVTGVAPAAAGGSTTGAMAACLLAAMS
                     YGFVSVYTKKFAAAANPRAIACSSQLMAGLLLLPLTPLAPPPGAVTVIIAVNMILFAL
                     LSSGVAFLLYFRLIADVGPTKSLTVTFLMPAFTMIWAALFLGETVTALMVV"
     gene            complement(932..1387)
                     /locus_tag="VE170_08445"
     CDS             complement(932..1387)
                     /locus_tag="VE170_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15505.1"
                     /translation="MERSIGADNEVIANFPGVTFGIHICRGNRQSMWHREGKYDAIAE
                     KLFNGLNHQRLLLEYDTARAGGFEPLRFVPKGKIAVLGLITTKLGRVETVDELRHRID
                     DAARFLPLDQLALSPQCGFASSIRGNLLSEDEQFRKLDVMLETARRIWG"
     gene            complement(1354..2064)
                     /locus_tag="VE170_08450"
     CDS             complement(1354..2064)
                     /locus_tag="VE170_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15506.1"
                     /translation="MDLNSLRVDLVGSLLRPEKLKQAFSDYGQGKASEADLAGAQDAA
                     IEDVVAKQAAHDLPIVVDGEFRRTSFMESFAVVAGVEEWQAGARTYHDILARKDEGKE
                     GTHKGQDPILLNRKRVTARLKLIRNTLVDDFNFAQSLTDRPVKVTLIGPDRIQQCYDS
                     EGSKAVYPSTDEFLRDVIAVERQMLGELTQAGCRYVGIDGPGYTAYDDADSIAAMRAP
                     AARSRWRLWSARLAPTMK"
BASE COUNT          505 a          669 c          598 g          449 t
ORIGIN      
        1 ctacgaccat caaggcggtg acagtttcgc cgagaaataa cgccgcccaa atcatcgtaa
       61 aagccggcat caaaaacgtg acggtgagag atttggttgg accgacatcg gcgatcaagc
      121 ggaaatacag caaaaacgcc acgccgctac tcaataatgc aaataaaatc atgttcactg
      181 cgataatgac cgtcaccgcc cccggtggcg gcgccaacgg ggtcaatggc agcaacaata
      241 gtcccgccat caattgactg ctgcaggcga ttgcccgtgg attcgccgcg gcggcgaact
      301 tcttggtata aacgctgacg aatccgtaac tcatggccgc taacaggcaa gcggccattg
      361 ccccggtcgt tgacccgcct gccgccgcgg gcgcgacacc ggtgacgaga gcgacaccgg
      421 cgatgccgag aacaaagccg agacacttcg gcaaggtgat cttctcgccg agccacagtc
      481 ctgcgcatag tgcgccaaag aatggcgagg tcgcgttaag aatcgccgag taggaggccg
      541 gaatatgcag cgcggcaaag gcgaagaggg caaatggcag cgcggcattg aacgcgccaa
      601 tgattagata ctgcttccag aaccgtcgcc agccgacatt gagaccgacg gtacggcaat
      661 aaatcgccag cgcgccccaa gcgatcagca cgcgcaaatc ggcggtgacg atcggtccga
      721 ataccggcgc taccactcgg agaaagacga acgacccacc ccagatcgcc gcgagcagca
      781 ccagccgaaa gatatcgctc gcgcgcatga ggtcggaggt gaatcaggaa cagctagtag
      841 tgcgagttta agtagggacg gtcaacgttc tctggttaca actgggattc caacattgaa
      901 ccttgaactt ttgaaccctt gaactaaatt tttaacccca aatccgtcgc gccgtctcga
      961 gcatgacatc gagcttacga aactgctcgt cctcgctcag caggttgccg cgaatgctcg
     1021 aggcaaaacc gcactgcggg ctcagcgcca actgatccaa cggcaggaag cgcgccgcgt
     1081 catcgatccg atgacgcaat tcgtccacgg tctcgacccg gcctagtttg gtggtgatca
     1141 aacccagcac cgcgattttt cccttgggca cgaaccgcag cggctcgaag ccgccggcgc
     1201 gcgccgtgtc gtattccaac agcaagcgtt gatggttgag gccgttgaat agtttctcgg
     1261 cgatggcgtc gtacttccct tcgcgatgcc acatgctttg ccggttgcct cggcaaatgt
     1321 ggatgccgaa agtcacgcca ggaaaattcg caatcacttc attgtcggcg ccaatcgagc
     1381 gctccatagt cgccatcggc tcctcgccgc gggcgcgcgc atcgcggcga ttgaatctgc
     1441 gtcgtcatag gctgtgtacc cggggccgtc aatgccgacg taacggcagc cggcttgggt
     1501 taactcacct agcatctggc gctctacggc aatcacatcg cgcaaaaatt catcggtcga
     1561 tggatagacc gcttttgatc cttcactatc gtaacactgc tgtattcgat ccgggccgat
     1621 caacgtcact ttcaccggcc gatcggtcaa gctctgggca aagttaaaat cgtcgaccaa
     1681 ggtattgcga atcaacttta accgcgcggt gacccgcttg cgattgagca ggatcggatc
     1741 ttggccttta tgcgtgcctt ccttgccttc gtctttgcgc gccaagatgt cgtgataggt
     1801 acgcgctccc gcttgccact cctcgacccc ggcgaccacg gcaaacgact ccatgaagct
     1861 ggtgcggcga aactcgccgt cgacgacgat cggcagatca tgcgccgctt gtttggcgac
     1921 gacatcttca atcgccgcgt cctgtgcgcc ggctaaatcc gcctcgctcg ccttaccctg
     1981 gccgtagtcg gaaaaggcct gtttaagttt ttccggtcgc agtaagctgc cgaccaagtc
     2041 tacccgcagt gagttcaggt ccactcatgt ccctttctaa gtatatcgtt aaccaagagg
     2101 gctgggttca atcagcttgc gcacattgcg ccctttgcgg tcgatacttt gagtctgcaa
     2161 cccttgatca ccccaattcc ttgatcgcct gctgcaaata actctggtcg atgtatttta
     2221 a
//
LOCUS       DASQKS010000323         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2698344, whole genome shotgun sequence.
ACCESSION   DASQKS010000323 DASQKS010000000
VERSION     DASQKS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698344"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2426
                     /locus_tag="VE170_08455"
     CDS             <1..2426
                     /locus_tag="VE170_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ15507.1"
                     /translation="EFENIIIRESATGAQIRVKDVGRAELGSQNYDSFGRLDGKPSGT
                     MALYLLPGANQLKASEAIYETLRQGKVLFPPDMDYKIVYDTTPAVNASIEGIVHTFIE
                     AIILVTLVVFIFLQNLRATIIPLLTVPVSLIGTFIFFPMLGFSVNTLSMFGLVLAIGI
                     VVDDAIVVVEAVMHHIEHGLNPKEATIQAMKEVSAPVIGIALILSAVFVPVAFLGGLT
                     GQMYKQFALTIAISVLLSAFAALSLAPALCAMLLKAHHGPARGPLGKFFGWFNRVFDR
                     TTTSYMGVVQLLVRRSILTIVIIAAVAVGAGLFGAALPAGFIPEEDQGIFGVNVTLPP
                     AASLERTGRVLSQVEQILAKTEGVDSFSTIGGYGVVTSTYQPNFGTIFVRMKPWEERS
                     SDALHVRGIMANVQRQVANISEAIVFPFNIPTISGFGASAGFNFLLQDRSGALTVQQL
                     GDYTRQFMDAARQRPELGNLFTSFDPRYPQVKVDLDREKARKLGVPINEAFQALSASM
                     GGSYVNDFNRFGRLFRVYVQSEADYRRKPKDIGDIYVRSRTTNTMIPLSTLINITSEG
                     GTEITVRFNLFRSVEINGVSGRGYTSGQSLAALEEVFKQTMPKEMGFAYSSLSYQEKI
                     APPAGPTLGLAIVFVFLLLAAMYESWRLPWAVLLGSPLVALGSFFGVWLMGYDNNVYV
                     QVGNIMLIGLAAKNAILIVEFAKAKHDEGMPVQEAALESARLRFRPILMTAFAFILGV
                     VPLMIANGAGAGAQNVMGTAVFWGMLVATFLGVFLIPGNFSFVEGLGRKKKPVPAVSP
                     TAPAPEGAH"
     gene            2428..>3348
                     /locus_tag="VE170_08460"
     CDS             2428..>3348
                     /locus_tag="VE170_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYJ15508.1"
                     /translation="MKRSVFVTVVGLLLSGCTLGPDYLRPRLLIPENHRGVVGAPAAE
                     SLADLPWWELFRDPVLQDLTRESLQQNFDLRIAVARVEQARAQVGVTRSFLFPQVNFN
                     ANGTLQQVSRASEPPQSLGTDRTFRNLLLGFGLAWEIDVFGRIQRETEAATAVYFATE
                     QARRGVLITLVADVAQSYFNLRELDLELEIARRTLRLNDETVDFYRRRLDGGVSNRLE
                     FDQSIANRSRTASTIPDLEARIAIQENQINLLLGRNPGPIPRGAVLTDQYYPPNIPAG
                     LPAALLERRPDVKAAEDLLVAANADIGAAKA"
BASE COUNT          604 a         1060 c          974 g          710 t
ORIGIN      
        1 acgaatttga gaacatcatc atccgcgaga gtgccaccgg cgcgcagatc cgcgtcaagg
       61 acgtcgggcg cgccgaactc ggctcgcaaa actacgactc cttcggccgg ctcgacggca
      121 aaccatccgg cacgatggcc ctctatctgc tgccgggcgc aaaccagctc aaggcctccg
      181 aagcgatcta tgagaccttg cgccagggga aggtgctgtt tccgcccgat atggactaca
      241 aaatcgtcta tgacacaacg ccggcggtca atgcctccat cgaaggcatc gtccatacct
      301 tcatcgaggc gatcatcctg gtcaccttgg tggttttcat ctttctgcaa aacctgcggg
      361 cgacgatcat cccgcttctc accgtgcccg tgtcgctgat cggcacgttc atttttttcc
      421 cgatgctcgg cttctcggtg aatacgcttt cgatgttcgg cctggtgctc gccatcggca
      481 tcgtcgtcga cgacgccatc gtcgtggtcg aagccgtgat gcatcacatc gaacatgggc
      541 tgaacccgaa ggaagcgacc atccaggcga tgaaggaagt ctcagctccg gttatcggca
      601 tcgcgcttat cctttcagcc gtcttcgtgc cggtcgcctt tctcggcggt ctcaccggac
      661 agatgtacaa acaattcgcc ttgaccatcg cgatttcggt gttgctctcc gctttcgccg
      721 cgctgtcgct cgctccggcg ctctgcgcca tgctgctcaa ggcgcatcac ggcccggcca
      781 ggggcccgct gggaaaattc ttcggctggt tcaaccgggt gttcgatcgc accacgacct
      841 catacatggg cgtcgtacag ctgctggtgc gacggtcgat actgaccatc gtcatcatcg
      901 ccgcggtggc cgtcggcgcc ggccttttcg gcgccgcgct gcccgcgggc ttcatccccg
      961 aagaagacca aggcatcttc ggtgtcaacg tgaccttgcc gccggcggcg tctctcgaac
     1021 gtacgggcag ggtgctatcc caggtcgagc agatcctggc caaaaccgaa ggcgtcgact
     1081 ctttctcgac cattggcggc tacggcgtgg tcacgagcac ctaccaaccc aacttcggca
     1141 cgattttcgt ccgcatgaag ccgtgggaag agcgctcttc cgatgcgctc catgtgcgcg
     1201 gcatcatggc taatgtgcag aggcaggtgg cgaacatttc cgaggcgatc gtctttccat
     1261 tcaacattcc gacgatctcc ggctttggcg cgtcggccgg gtttaacttc ttgctgcagg
     1321 accgcagcgg cgcgcttacc gtgcagcaac tcggcgatta cacccgtcag ttcatggacg
     1381 cggcgcgcca gcggcctgag ctcggcaacc tgttcacctc gttcgatccg cgttaccccc
     1441 aggtcaaggt ggatctcgac cgcgagaagg cgcgcaagct cggcgtgccg atcaacgaag
     1501 cgtttcaagc gctatcggca agcatgggcg gcagctatgt caacgacttc aaccgtttcg
     1561 gccgtctgtt ccgggtctac gtgcagtccg aggccgacta ccgccgcaag cccaaagaca
     1621 tcggtgatat ttatgtgcgc agccgcacca ccaatacgat gatcccgctt tccaccctga
     1681 ttaacattac ctctgaaggc ggcaccgaga tcacggtgcg tttcaacctg tttcgctcgg
     1741 tcgagattaa tggcgtatcg ggccgcggtt atacctccgg ccagtccctg gcggcgctgg
     1801 aggaggtctt caagcagacc atgcccaagg aaatgggctt cgcctattcc agtctttcct
     1861 atcaggaaaa aatcgcgccg ccggcgggac cgactctagg actcgcgatc gtgttcgtgt
     1921 ttctcctgct ggccgctatg tatgagagct ggcgcctgcc ctgggcggtt ttgctgggct
     1981 cgccgctggt cgccctgggc tcgtttttcg gcgtctggct gatgggctac gacaacaacg
     2041 tttatgtcca agtcggcaat atcatgctga tcggcctcgc cgccaaaaac gcgattctga
     2101 tcgtcgaatt cgccaaagcg aaacacgatg aaggcatgcc ggtgcaggag gccgccctgg
     2161 aatccgcccg gctgcgcttc cggccgattc tcatgaccgc gtttgccttt atcctgggtg
     2221 tcgtaccgct gatgatcgcc aacggcgccg gtgccggcgc gcagaacgtc atgggtaccg
     2281 cggtcttctg gggaatgttg gtcgctacgt ttctgggagt gtttctgata cccggcaact
     2341 tctccttcgt cgagggactc ggaagaaaaa agaaaccggt cccggcggtg agtcccaccg
     2401 cgccagcacc cgagggagcg cattgaaatg aagcgaagcg tttttgtcac cgtcgttggc
     2461 ttgcttcttt ccggttgcac tctcgggccg gactatctcc ggccgagact tctgattccc
     2521 gaaaaccacc gtggagttgt cggcgcgccg gccgccgagt ccctcgccga tcttccctgg
     2581 tgggagcttt tccgcgatcc ggtattgcag gatcttactc gcgaatcgtt gcaacaaaat
     2641 tttgacctgc gcattgccgt ggcccgggtc gagcaggcgc gggcgcaggt cggtgtcact
     2701 cgatcgtttc tctttccgca agtaaacttc aacgccaatg gcaccctgca acaggtgtcg
     2761 cgggcgagcg aaccgccgca gtcgttgggc accgaccgga catttagaaa cctgctattg
     2821 ggtttcggtc tggcctggga gatcgatgtc tttggcagaa tccaacgcga aaccgaagct
     2881 gccacggcgg tttattttgc cacggaacag gcgcggcgcg gagtcttgat cactctagtg
     2941 gccgatgtcg cccagtccta tttcaatttg cgcgagctcg atttggaact ggagatagcc
     3001 cggcgcacgc tccggcttaa cgacgagacc gtggactttt accgcaggcg gctcgacggc
     3061 ggggtctcga accggctgga gttcgatcag tcgatcgcca accgttcgcg cacggcgtcg
     3121 acgatcccgg acctcgaggc ccggattgcg atacaggaaa accagatcaa tctgctgctc
     3181 ggccgcaacc ccggaccgat cccgcgcggc gcggttctca ccgaccagta ctatccgccc
     3241 aacatcccgg cgggcttgcc cgcggcgctg ctcgagcgcc ggcccgatgt caaagcggcg
     3301 gaggacttgc tggttgccgc caacgccgat atcggcgctg ccaaagcg
//
LOCUS       DASQKS010000324         6947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4788985, whole genome shotgun sequence.
ACCESSION   DASQKS010000324 DASQKS010000000
VERSION     DASQKS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4788985"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1035)
                     /locus_tag="VE170_08465"
     CDS             complement(352..1035)
                     /locus_tag="VE170_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ15509.1"
                     /translation="MASNTGNRQFVDSVIREIIQPGMDRLMESRYFSDLRAGKLTTRR
                     IQGFALQHYIHNMGILKAFALGATQHAADDRTFTAYATAMGEELTHPNMCKTLGFSLG
                     LTEADFDKAVPVRGALVHTAVCIHGIFLVSVAEMRAMALSNETMVQGYAAEFDDYLSK
                     DPYNFSEKAREFFIVHKGADVEHTERAAAAIAASATSDEDQEKVRTICRNMAGFKLGK
                     FDSIYDEYA"
     gene            1820..2716
                     /locus_tag="VE170_08470"
     CDS             1820..2716
                     /locus_tag="VE170_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ15510.1"
                     /translation="MRTPSSLITLGTAGGPSARPGRAQSSNLLTVNGTHYVIDAGDGV
                     ARRLSRAGIDFRNIGTIFITHHHDDHTAGLGTLMSLSWDRQRTQPINVYGPPRTEELV
                     KAAVHYYGISAEIRIADGGRSKPIAQVFYGHDRGAGAFYQDANIKVTAIENTHFSFHK
                     GEAAGKHKSYSYRFETPDRVIVFTGDTGPCTAVTALAKDAEILVTETSSCEDRKQAMM
                     ADGRWQAMTSAEQIGIMQQATQGHMSLEAIGNTAAQAKVKTVVLSHLTTRPGLDDYTP
                     WADEVRKYFPGSVVIANDLMEF"
     gene            3095..3469
                     /locus_tag="VE170_08475"
     CDS             3095..3469
                     /locus_tag="VE170_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15511.1"
                     /translation="MSIYATLWSLKFPRYGDDFFDCEWIRMTAQGVPGHIGTPTPGHG
                     YENGDPYADFLPPPVEIDADGASEFMRAVMFVSEETKKGTSRAGQEYLEPLLVLTGRE
                     YAAIKFDELHTRICDALRDRGP"
     gene            complement(3624..5144)
                     /locus_tag="VE170_08480"
     CDS             complement(3624..5144)
                     /locus_tag="VE170_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYJ15512.1"
                     /translation="MPHQDRLNIAVFIDFDNIEIGIKSTLGENFDVGAILDALKERGE
                     VVTKIAYADWTRAGEYSRHLTQHAIRLVQRNLTPGGDKNGADINLALDALEMAFTHPH
                     INAYVIVGGDSDFLSLVEKLKQYDKKVFVVGGRAFTSIILQRNCHEFIAYENLTGGRR
                     SVAKGGRPVAPAASAPIAQAFPIVRRALKILSDREVSPQTGLLKSTLLQLDSTFSERT
                     YGASSFLDFVEKLAQSGLAQLKHTGRSVMVELNEKFHESESSTAAAVAASGPVALAEA
                     RDEVRAAAPEAETEPGVVMAKPVQASRPAPSEALVVGAGAQADGVRLVGQILTAATAA
                     RWPMYLRNVKQLLRAAEGGFDERRYGFMGLIDLLRGCQSEGLVRLERDRRGGLRVFKG
                     PALQETGPSPAIAPSQEVVEAEPLYTQPLETVDRFELEERAEEDRFNVEPMPIDTTAE
                     LLGRAKQRRPRARVTAAAPPAAARVPRKAASKKAAPGASTRSRKPARSAAPGKKEN"
     gene            5491..5829
                     /locus_tag="VE170_08485"
                     /pseudo
     CDS             5491..5829
                     /locus_tag="VE170_08485"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
     gene            complement(6183..6512)
                     /locus_tag="VE170_08490"
     CDS             complement(6183..6512)
                     /locus_tag="VE170_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HYJ15513.1"
                     /translation="MKKSKKRRYRSDAFAAIHETAKAMYRVGGISKTTMREFDESCLE
                     TVESIKPAQIRALRQQANVSQPVFARYMNVSKNLVSAWERGERKPGGPALRLLGVIRK
                     HGLETII"
     gene            complement(6499..6876)
                     /locus_tag="VE170_08495"
     CDS             complement(6499..6876)
                     /locus_tag="VE170_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYJ15514.1"
                     /translation="MRIFKNAWFSRFARRENIKDAVLVDAVARAERGVIDADLGGGVI
                     KQRIARPGQGKSGGYRSFILFRKGTQAFFVYGFAKSDRENIEYDELEKFKFLAKAWFS
                     LSDKQIEAMTRDKALTEVKHEEK"
BASE COUNT         1476 a         2025 c         1989 g         1457 t
ORIGIN      
        1 taagacctat ggtgttagcg acaacaataa gattcgtctc cgcgcaaagg ttcttgtcca
       61 cttgtccgtc cggcgaatga ccttccgttt tttctcaaga gaaaaacgcc cagcagattg
      121 ctgaagaaaa gtctcttgca ggctgcacaa aaagattcag aggcgagggc gcgagaaatt
      181 gacgagcgga gtcgtgcatc ggcaatacgt tgacgcgagg cgatttggcg caacgaagca
      241 tatgggtctt ttcccgcagc ctgcttgaag aactgacgac gaacagccca ggattaattg
      301 cccaacgctt cggagcaatc tcgcgggatc gagtcgacgg cggtactgca gttaggcgta
      361 ttcgtcgtag atgctgtcga acttgccgag tttgaacccg gccatgttgc ggcaaatcgt
      421 gcgaaccttc tcctgatcct cgtcgctggt tgcgctcgcc gcgatggcgg cggcggcgcg
      481 ctcggtgtgc tcgacgtcgg cgcccttgtg aacgatgaag aactcgcggg ctttttccga
      541 aaagttgtat ggatccttcg ataggtagtc gtcgaactcc gcggcgtaac cttggaccat
      601 cgtctcgttg gacagcgcca tggcacgcat ctcggcgacg gacaccaaaa atattccgtg
      661 aatgcatacc gctgtatgca ccagggcgcc gcgcaccggc acggccttgt cgaagtccgc
      721 ctccgtgagt cccagggaga agcccaaagt cttgcacatg ttggggtgag tcagttcttc
      781 gcccatggcg gtggcgtacg ccgtaaacgt ccggtcatcg gcggcatgtt gggtcgcccc
      841 aagtgcgaac gccttgagaa tgcccatatt gtggatgtag tgctgcaaag caaagccttg
      901 gatccggcga gtagtgagct tgcccgcgcg caggtcggag aagtaacggc tctccatcag
      961 ccggtccatg cccggctgga tgatctccct gatgacggag tcgacgaact gccggttgcc
     1021 ggtgttcgaa gccataggta gttctccttt tagaaaaatc gcaactgcgg cctggccaat
     1081 gagaactcga gaattctgct acggcgcatt tcaaaaatct ataaacagat tgcggccaac
     1141 attgccgcgg tggttggctt caaacaagtc tgcggtaatt ttttccgggg taacatgcgt
     1201 tcagctccat attaaaacta atggcatcta tgagccgctt actgtttgtg gccactatca
     1261 aaggtgaagc gataaagtca aatcagttta gcgaagtgcg gttcgattaa tttttcttgc
     1321 gccctttgcg ttctttgcgg ccactctttc gttcgaatgc agcgtcgatg catcaggtgc
     1381 tgaaaaaatc tctcgcaggc tgctcaaaaa gatccagagg cgaggcgcgc gagaaatcgc
     1441 cgagcggagg cgtacatcgg cagtacgttg acgcgaggcg attgagcgca acgaagcata
     1501 tgggtctttt tcagcagcct gctaggaaat ccgcttgcca gctttgcctg ccgccttgag
     1561 ccaagcgacc ttgcaatcat cacggcgcac cgccggctgc tcaatctggc ccgtgaactc
     1621 cagcagggca gtgttgttga cgagttaccg aacaggggaa gaaagtaaga ttcttctccg
     1681 cgcgacggtc tgagcctatt tgacagtccg gctaatgggc gacacctagg tgttcccgct
     1741 ggaccgcgcc gccggttgac cgcacaattg gacaacggca tggtagaaat agcacatgcc
     1801 atcaaaccgg aggctgcaag tgaggacccc ttcatcgctg atcacgctgg gaacagccgg
     1861 cggaccatcg gctcgacctg gccgggcaca atcgtcgaat cttctcaccg tcaacggaac
     1921 ccattatgtc atcgatgccg gtgatggagt cgcgcgccgc ctatctcgcg cgggcatcga
     1981 ctttcgcaat atcggcacaa tctttatcac ccatcatcac gacgatcata cggcgggtct
     2041 cggcacgctc atgtcactgt cgtgggatcg ccaacgcacc cagccgatca acgtctacgg
     2101 tccgccgcgc acggaagaat tggtcaaagc cgcggtgcat tattacggca tcagcgccga
     2161 aatcaggatc gccgacggcg ggcgcagcaa accgattgct caagtattct acggccatga
     2221 tcgaggcgcc ggggcatttt accaggatgc caacatcaaa gtgaccgcga tcgaaaacac
     2281 ccacttcagc tttcacaaag gcgaagcggc cggcaagcac aagtcgtatt cgtatcgttt
     2341 cgaaaccccc gatcgcgtca tcgttttcac cggcgacacc ggcccctgta ctgccgtcac
     2401 cgcgctggcc aaagatgctg agattctcgt aaccgaaacg tcatcctgtg aagaccgcaa
     2461 gcaagcgatg atggccgatg gccgatggca agcaatgact tcagcggagc agatcggcat
     2521 catgcagcaa gcgacccagg gccatatgtc gttggaagcc atcggcaaca cggccgcgca
     2581 ggcaaaagtc aaaaccgttg tgctatcgca tctaaccacg cgccccggtc tggacgatta
     2641 cacgccctgg gccgacgagg tcagaaaata ttttcccgga tcggtagtaa tcgccaatga
     2701 tctgatggag ttttagccca aagtatagat cgtgaaagtc tgctcgctca cgctgctcgt
     2761 gcttatcacg ttcttgagca accgcagccg gcgccgccag ctacgaaaca caaaaagtcg
     2821 agggccattg gcgccaaggt gaagtcgtct atcgggacaa cctggtgaaa atcgttgtcg
     2881 atgttgcgga ttccgccgcg catcgaaatt ggatgaagca atacaaagct cgttgaaaaa
     2941 cctgtctcga acagctcgaa ctttggatgg tcagtggtcg tatagagatt gactaaccga
     3001 cggcgttttt acgccgtgat attcaggggt atttgattcg ccactcgaat acactggacc
     3061 gaaactgcgc ccaggccctt agcgagtttt gttgatgagc atttacgcaa ctctctggtc
     3121 acttaaattt ccacggtacg gcgacgactt tttcgattgc gagtggattc ggatgaccgc
     3181 gcaaggggtt cccggacata tcggcacgcc gacaccgggt catggttacg aaaatggcga
     3241 tccctacgct gactttctac cgccgccggt ggagattgat gcggatggcg ctagcgagtt
     3301 catgcgcgcc gtgatgtttg tcagcgagga aactaagaaa ggtacgagtc gcgcagggca
     3361 ggaatatcta gagccattgc tcgttctgac ggggcgtgaa tacgcagcta tcaagttcga
     3421 tgaattgcac acgcgcatct gcgatgcgct gagagaccgc ggtccatgaa tcgttcacag
     3481 cggaattcaa cttatccctt cggacagacg ggtgatcgac ggccataacg ataaaagttg
     3541 gcacgagccg cttaaagagc taaaaagccg gcttgacccc attggggttc cccgcccaag
     3601 ggaatgaaga gagaccgggt gcgtcagttc tcctttttgc caggggccgc ggatcgggcc
     3661 ggcttcctcg accgcgtgga cgcgcccggc gcggccttct tcgaggccgc ctttcgcggg
     3721 acacgcgctg ccgcgggggg ggcggcggct gtgacgcggg cgcgcggacg tctctgcttg
     3781 gcgcgaccga ggagctcggc cgtcgtgtcg atcggcatcg gctcgacatt gaaacggtcc
     3841 tcctcggccc gctcttccag ctcgaatcga tccacggtct cgaggggttg cgtatacagg
     3901 ggctcggctt ccaccacttc ttgggacggg gcgatcgcgg gcgatgggcc ggtctcttgc
     3961 aacgccggcc ccttgaacac gcgcagccct cctcgacgat cccgctcgag tcgcacgagg
     4021 ccctcactct gacatccgcg cagcagatcg atcagtccca tgaacccgta tcggcgctcg
     4081 tcgaagccgc cctcggcggc tcgtaagagc tgcttgacgt tgcgcaggta cataggccac
     4141 cgtgccgccg tggcagcggt caggatctgg cccaccaggc ggactccgtc ggcctgagct
     4201 cccgccccta caactagtgc ctcgctcggg gcgggccggg acgcctgcac gggcttggcc
     4261 atcaccaccc ctggctcggt ctcggcctcg ggcgcagccg cgcgaacctc gtcgcgcgcc
     4321 tccgctaggg cgacggggcc gctcgcggcg acggcggcag cggtcgaaga ctcgctctcg
     4381 tggaacttct cgttcagctc gaccatgacg cttcgaccgg tgtgcttgag ctgggcgaga
     4441 cccgattgcg cgagcttctc gacgaaatcg aggaatgacg acgcaccata ggtccgctcg
     4501 gagaaggtcg agtcgagctg gagcagcgtg ctcttgagca gaccggtctg cggcgagact
     4561 tcccggtcgg acaggatctt tagcgcgcgc cgcacgatgg ggaacgcctg cgcgatgggc
     4621 gcggaggcag cgggcgccac cgggcgaccg cccttggcga ccgagcggcg gccgcccgtc
     4681 aggttctcgt aggcgatgaa ctcgtggcag ttgcgctgga ggatgatgct ggtgaacgcc
     4741 cggccaccaa cgacgaacac cttcttgtca tattgcttga gcttctcgac cagcgagagg
     4801 aagtcactgt cgccgccgac gatcacgtac gcgttgatgt gcgggtgcgt gaaggccatt
     4861 tcgagggcat cgagcgccag gttgatgtcg gcgccgtttt tgtcgccgcc gggcgtcaag
     4921 ttgcgctgca ccaggcggat ggcgtgctgg gtgaggtggc ggctgtactc gcccgcgcgc
     4981 gtccagtcgg cgtaagcgat cttggtgacc acctcgccgc gctccttcag ggcgtcgagg
     5041 atggcgccga cgtcgaaatt ctcaccgagc gtcgatttga tgccgatctc gatgttgtcg
     5101 aagtcgatga atacagcgat gttgagacgg tcctgatgag gcaaatgaac ttcctttcaa
     5161 gcttctattc tatgccaaga gcgatctcgc ggccctctcc gccccggtcg gtcacctcgt
     5221 actgtttata ggagctttaa accacctgtt tactgaccgg agatttatcg ctgaacgatc
     5281 gaggatgtca atcgtcggcg agtcttgtga aattcgcggt tttgcttcgg ctggctccgg
     5341 cgatgcagcg gtcggagagg cgcgaagtgt tccttgtcag gttgctcgct cgaactcaga
     5401 gcgagccccg ccgttggctt gccttggcaa gtggtttggg ctcctttaaa tcaactacat
     5461 ccccaatagg cgtgtacgtt cctaccgaag cgcaacatgg ccaacgacta cctgctcgat
     5521 cgcgagatcc agcgcactac cagctacacc ggcatctggg gcgtcaacga ccaggatcgt
     5581 gcgatacagg aaagcatggg cccgatctac gaccgccgca aggaacacct cggcaccagc
     5641 gaccaagcga tcatcacggc gcgccgccgg ctgctgaatc tcgcccgtga actccagcac
     5701 ggcatcgagc ccttccccgc gtcccacgga gacatctatc gagtgcgcgc catggatgtg
     5761 aacacggcgg tcgacgactt cgacgccgtg atcgccagtc acggcagcgg cttgctcgcc
     5821 aaggtgtagg atcgccatag gtggctaccc ggcggccgtt cgctaacctt tcaattggcc
     5881 gaatcctaac aggttgctgg gaaaagcctc ttacacgctg tttaaaaaga gccagaggca
     5941 aggcgcgtga aaaatcgacg agcggaggcg tacatcggca gtacgtcgac gcaaggcgat
     6001 tgagggcaac gaagcatatg ggtctttttc agcagcctga taaggatacg attagccgat
     6061 gcccgacaaa gccctaccag cgcttcgccg agccgcggct cacgaagcac tggccgattc
     6121 gccaatcgac catgcacagc aggcctcctt caaaagcgaa cgataattca ccgagcgccg
     6181 gtctagataa tcgtctcaag tccgtgtttt cgaataacgc cgaggagccg cagcgccggt
     6241 ccaccgggtt ttctttcgcc gcgttcccaa gccgaaacca gatttttcga cacgttcatg
     6301 taacgcgcaa agaccggttg cgaaacattc gcctgttgcc gcagggcgcg tatctgcgca
     6361 ggctttatgc tctccaccgt ctcaaggcaa gactcgtcaa attcacgcat cgtcgtcttg
     6421 ctgataccgc cgacacggta catagctttt gctgtctcat ggatcgccgc aaaggcatcc
     6481 gaacgatagc ggcgcttttt acttttcttc atgtttgacc tccgttagtg ctttgtccct
     6541 ggtcatggct tcgatttgct tatctgaaag cgaaaaccat gcctttgcta ggaatttgaa
     6601 tttctctaac tcatcgtatt cgatattttc gcgatcactc ttcgcaaagc cgtatacaaa
     6661 aaatgcctgt gttcctttgc ggaatagaat aaagctccga taaccgcctg acttaccttg
     6721 acccggccgg gcaatccgct gcttgatcac tcctccaccg agatcggcat caatgacacc
     6781 gcgctcagcc cgcgcaacgg catcaactag aaccgcgtcc ttgatatttt cccgccgcgc
     6841 aaaccggcta aaccatgcat ttttaaatat ccgcaaacga cctctcacag tttctatgac
     6901 aatatcatac tttgtgtgat agaagaagat tgctgtcctt gccaaca
//
LOCUS       DASQKS010000325         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1428671, whole genome shotgun sequence.
ACCESSION   DASQKS010000325 DASQKS010000000
VERSION     DASQKS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428671"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1081)
                     /locus_tag="VE170_08500"
     CDS             complement(89..1081)
                     /locus_tag="VE170_08500"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15515.1"
                     /translation="MVRTLAVVVILTSALAGTSPADAQRIRAASGGLSIIHSLLWVTY
                     EQKLLKKYGLDLEYIAIENGTVGMQALLANEAQFLFSTSSLAVSANLRGADVTVVAGG
                     LNFIPDKLIVRPEINKPEDLIGKRLAISRFGSSSETSAKLTLEKVGVKPESVSLIQLG
                     GVSTRQVALMAGQVQATVLSDPQATASTQGGMKLWVDLSESKWGLPRICFNCFMAKRS
                     FLDSNREAAGNFLKAVIEGLYLMKKDKPLGVRLIKKYLRLNDEAASIGYDFYIGKHGE
                     GMLSLPERRGMEFIIAEVAKANPAASKATPESLRLVEPSFLDEIKKSGFVERVR"
     gene            complement(1100..1822)
                     /locus_tag="VE170_08505"
     CDS             complement(1100..1822)
                     /locus_tag="VE170_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HYJ15516.1"
                     /translation="MNPFQRENVKFLAFDVFGTVVDWRSSVIAEGEGLGTAKGLTIDW
                     TAFADAWRSIYRPYMDKVQNGELPWTKLDDLHCVMLGETLKRFALHDLSDAEKENLNR
                     VWHRLTPWPDAVPGLQRLKSRFLIAPLSNGNISLLTNMAKHAGLPWDCILSAENVRRY
                     KPDPSVYLLAPQLFDLKPEQVMMVAAHEHDLQSARKHGLRTAYVHRPLEHGPGKASEM
                     PPRDRYEIVANDFLDLADQLSA"
     gene            complement(1833..2981)
                     /locus_tag="VE170_08510"
     CDS             complement(1833..2981)
                     /locus_tag="VE170_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYJ15517.1"
                     /translation="MTDSLFPRFSDSEYTRRFNVIRAAMSQGNLDAILVSGARGSSEV
                     AYLSNYQAQSPCWLLFPRDGDATVFIHFFNHQPCAKAQSIMADVRWYGPTPMPTLVEE
                     IKRRGLSKSKIGLVSMRSMAYGNVIELQRQFPAAEFVEFGPQFAKIRRVRSEEELVYL
                     RRSGYLTDLACEALENNLRAGLTEKDVLAIVYNAYVKNGGDPGIHFIATTNMDKPDRF
                     VPWQRQTSRVLETGSVVITELTVSYWGYSTQIHRPFAIGKEPTPTYRRLFDAAYECYE
                     SVRKICKPGTTSEQIIAATSAVEANGFTSFDSVFHGEAGKSPELGTSSAAHPVEKWTL
                     EENMVHVIQPNPITKDFTAGLQLGAAVVVKPGGGEALHNYPFKFPVCG"
     gene            complement(3016..>3364)
                     /locus_tag="VE170_08515"
     CDS             complement(3016..>3364)
                     /locus_tag="VE170_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HYJ15518.1"
                     /translation="SWTPPNWIVGPVWTGLYIAIAIAGWAVWRSKAISVTKPILLWLL
                     QVILNGLWSWLFFGLHRPDLALIDITALLITICCFIATAHGASPVAAWLFVPYALWVG
                     FATALNFAIWQLN"
BASE COUNT          707 a          920 c          985 g          752 t
ORIGIN      
        1 tgaaaacata ttcatcgccg caggaatgag acgtcaaaaa tggccgcgat tgactcaggc
       61 aggtaataaa gtagcgtcat gcgagatttt atcgtacgcg ttcgacaaat ccgctctttt
      121 taatttcatc taaaaagctc ggttccacca gccgcaggct ttcgggcgtc gctttgctcg
      181 ccgcaggatt ggccttcgcg acttcggcga tgatgaactc catgccgcgg cgttcaggca
      241 ggctgagcat gccttcgccg tgtttgccga tgtagaaatc gtagccgatg gacgcggctt
      301 cgtcattgag gcgcaggtat ttcttgatta ggcgcacgcc gaggggcttg tcctttttca
      361 tcaggtagag accctcgatg acggccttga gaaaattgcc ggcggcttcg cgattgctgt
      421 cgagaaagct gcgcttggcc ataaaacagt taaagcaaat gcgcggcagc ccccatttgc
      481 tctcggacag gtcgacccat aacttcatgc cgccttgggt ggacgcggtc gcttgcggat
      541 cgctgagaac agtcgcttgt acctgtccgg ccattaacgc cacctggcgc gtcgatacgc
      601 cgccgagttg aatcagtgaa acgctctccg gcttgacgcc gactttttcc agagtcagtt
      661 tagcgctggt ctccgacgac gagccaaaac ggctgatcgc caagcgcttg ccgatcagat
      721 cctcgggctt gttgatctcc ggtcggacaa tcagcttgtc gggaatgaaa ttcagtccac
      781 cggcgaccac tgtgacatcg gcgccgcgca ggttggcact gaccgcgagc gaagaggtgg
      841 agaacagaaa ttgcgcctcg ttggcgagca gcgcctgcat gccgacggtg ccgttttcaa
      901 tggcgatgta ttcgagatcg aggccatact ttttcaacag cttttgctcg taggtaaccc
      961 acagcagtga atgaatgatc gacagcccgc cgctggcggc gcggatgcgt tgcgcgtcgg
     1021 cgggggacgt cccggccaac gccgacgtta aaataacaac aacggcaagg gttcgcacca
     1081 tcacagaatg ctccttcact tacgcggata gttgatcggc gaggtcgaga aagtcattgg
     1141 cgacaatttc gtatcgatcc ctcggcggca tctcgcttgc cttgcccggg ccgtgctcca
     1201 acggccggtg aacatacgcc gtgcgcagtc cgtgcttacg tgcggattgc agatcatgct
     1261 catgagccgc gaccatcatg acttgctcag gtttcagatc gaacaattga ggcgcgagta
     1321 aatagaccga tggatcgggc ttgtagcgtc ggacgttttc cgcggacaaa atacagtccc
     1381 agggtaagcc ggcgtgcttg gccatgttcg ttaagagaga aatattgccg ttggacaagg
     1441 gcgcgattag gaatcgcgat ttcaaccgct gcagcccagg aacggcatcg ggccatggcg
     1501 tgagccgatg ccagacgcga ttcaagtttt ccttttcagc gtcgctgaga tcgtgtaggg
     1561 cgaatcgctt caacgtctcg ccaagcatta cgcaatggag atcgtcgagc ttagtccacg
     1621 gcaattcgcc gttctgcacc ttgtccatgt aaggccgata gatcgaacgc catgcgtctg
     1681 cgaaggcggt ccagtcgatg gtgagcccct ttgctgtgcc gagtccttcg ccttcggcga
     1741 tcacgctcga gcgccagtcg acgactgtgc cgaagacatc gaaggcgagg aatttgacgt
     1801 tttctctttg gaacggattc attttgctgt ccttagccgc acacgggaaa tttgaacgga
     1861 tagttatgca gcgcttcgcc gccgccgggc ttcaccacca ccgcggcgcc gagttgcagc
     1921 ccggctgtga agtccttggt tatcggattg ggctggatga cgtggaccat gttttcttcc
     1981 aaggtccatt tctccactgg atgcgcggcc gagctggtgc cgagttccgg tgatttgccg
     2041 gcttcgccgt ggaagacgct atcgaagctg gtaaagccgt tggcttcgac tgccgaggtc
     2101 gcggcgatga tctgttcgct ggtggtgcca ggtttgcaga tcttgcgcac gctttcgtag
     2161 cattcgtagg cggcgtcgaa cagccggcgg taggttggtg tgggttcttt gccaatggca
     2221 aatggccgat ggatctgtgt cgagtagccc cagtagctga ccgtcagttc ggtgatcacc
     2281 acgctccctg tttcgagcac gcgggaggtc tgccgctgcc atgggacgaa gcggtctggt
     2341 ttatccatgt tcgtcgtcgc gatgaagtgg atgcccgggt cgccgccgtt tttcacgtag
     2401 gcgttataga cgatggcgag aacatctttt tcagttagac cggcgcgcag gttgttttcc
     2461 aatgcctcgc aggcgagatc ggtgagatag ccgcttctgc gcagatagac gagttcttct
     2521 tcgctgcgca ctcgcctaat cttggcgaac tgcgggccga attcgacaaa ctcggctgcg
     2581 ggaaactggc gctgcagctc aatcacgttg ccataggcca tgctgcgcat gctgaccagg
     2641 ccgatcttgc ttttcgacag gccgcgcctt ttgatttctt cgaccagtgt cggcattggc
     2701 gtcggaccat accatcgaac atctgccatg atcgattgcg ctttggcgca gggctggtga
     2761 ttgaaaaagt ggatgaaaac agtggcgtcg ccatcccgcg gaaacagcaa ccagcaaggc
     2821 gactgcgcct ggtagttcga cagataggct acttccgagg aaccgcgcgc gccggaaacc
     2881 aagatcgcat cgagattgcc ttggctcatc gccgcccgga taacgttgaa gcggcgggtg
     2941 tattcgctat ctgaaaaacg cggaaaaaga ctgtccgtca tgatttgttc cccattggga
     3001 gttctcggaa aatgactaat tcaactgcca tatcgcaaaa ttcaacgcgg tggcgaaacc
     3061 gacccatagc gcatagggaa cgaacagcca agcggcaacc gggctcgctc catgggccgt
     3121 ggcgataaag caacagattg ttatgagcaa tgcggtgata tcgatcagcg ctaggtccgg
     3181 ccggtgcaaa ccgaagaaaa gccatgacca aagtccgttc agaatcactt gcagcaacca
     3241 aagcagtatc ggcttggtaa cactgatagc tttggatcgc cacacggccc aaccggcgat
     3301 cgcgatggcg atatatagcc cggtccacac cggtccgacg atccaattgg gtggcgtcca
     3361 tgac
//
LOCUS       DASQKS010000326         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4790657, whole genome shotgun sequence.
ACCESSION   DASQKS010000326 DASQKS010000000
VERSION     DASQKS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4790657"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VE170_08520"
     CDS             complement(<1..1019)
                     /locus_tag="VE170_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ15519.1"
                     /translation="MAMPFRADNIGSLLRPAELLEARAALREGRMDEPQVRELEDRSI
                     LQALAMQKASGVDVFTDGEYRRDIFTADITKAMDGLVPGKPVVKFEWRGRGKELAAES
                     KEGNLQYIVGAKLTRKGRFTPNEAPFLKQHAPGPFKVCTPAAMQHAIMRYRPGVSDKF
                     YPTVHDMLQDVAAIMRHEVQALIDEGASYVQLDAPSYSNFFDQSRRAVLTESGINLTE
                     ALDAAIAADNAMIDGIKRNSDVAIGIHFCRGNKRSAWGAEGSYEPIAEKAFGSLKMDR
                     YLLEFDSDRAGGFEPLRFVPKGKTVVLGLITTKEPALESEDELMRRIDQAQRYVAVEN
                     LALST"
     gene            complement(1149..2309)
                     /locus_tag="VE170_08525"
     CDS             complement(1149..2309)
                     /locus_tag="VE170_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYJ15520.1"
                     /translation="MVQTAMKRLAQIRTDVVGSLLRPSHLKQAYADQDAGKITAEELR
                     RIEDEAIGHAVRLQEAIGLEVVTDGEFRRLNFQDSFAASVSGFDARPADLSFVESLSR
                     EGKPLQRWDPYHVADGAAILQRRPVSQKLRLQRNLPLEEYLFVSSVAQRPAKVTLVGP
                     DRISQRFDYQNSKSIYWSMEDFIADVAHIEREMIRGLVDAGCRYVQIDAPSYTAYVDA
                     PSLDEMKARGEDPVKNLMRSVNADNEILRLFDDVTFGIHLCRGNQRSMWHREGSYDAI
                     AEMLFTELGHDRFLLEYDSPRAGTFAPLRFVPKNKVVVLGLVSTKVPEIETVDDLKRR
                     IDAAAKYLPLEQLAISPQCGFASDVVGNLVSEDDQKRKLEVVVETARQVWEK"
     gene            complement(2356..>3202)
                     /locus_tag="VE170_08530"
     CDS             complement(2356..>3202)
                     /locus_tag="VE170_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15521.1"
                     /translation="PQGGLPADQTMDLVDLARVQRQWFSGPSDRLLTLGMAARSLSDS
                     PRGAVTTPTLRRDWDGARKLGLPITIHTGGAGRVALLEREAMLGPDVQLINPTAYDEA
                     DFARIVKARAHVCLSPFAEMRSSFVSSRLVDMLKHGILVSMSIDTAAISGNNDMFAHM
                     HALIDTQFVINKNPTSLSARQVLELATINGARDLDIADRVGSLTPGKRADLILVRATD
                     LNMAPLGDPVQAIVRSAQPHNVDTVVVDGRIVKRGGQMTALDVEKVVREASESLASLR
                     MRAGV"
BASE COUNT          656 a         1005 c          873 g          668 t
ORIGIN      
        1 tgggtgctga gggcgagatt ctccaccgca acataccgct gcgcttggtc gatgcggcgc
       61 atcagctcat cttcggattc cagcgcgggt tctttggtcg tgatcaaacc caacaccacg
      121 gtcttgccct tgggcacgaa gcgcaacggc tcaaagccgc cggcgcgatc gctgtcaaac
      181 tccagtaaat aacgatccat cttgagggag ccgaatgcct tctcggcgat cggctcgtag
      241 cttccctcgg cgccccaggc actgcgcttg ttgccgcggc agaaatgaat gccgatggcg
      301 acatcgctgt tgcgcttaat gccgtcgatc atggcgttgt ccgccgcgat cgcggcatcg
      361 agcgcttcag tcaaattgat gccgctctcg gtcaacaccg cgcgccggct ctgatcgaag
      421 aaattcgaat aggaaggcgc gtccagttgc acgtacgacg ccccctcatc gatcaacgcc
      481 tgcacttcat gacgcatgat cgcggcgaca tcctgcaaca tgtcatgcac cgtcggatag
      541 aacttatccg acacccctgg ccgatagcgc atgatcgcat gctgcatcgc cgccggcgtg
      601 cagaccttga atggcccggg cgcatgttgt ttcaagaacg gcgcctcatt gggggtaaat
      661 cggccttttc tcgtgagctt ggcgccgaca atgtactgga gatttccttc tttcgattcg
      721 gcggctaact ctttacctct gccgcgccat tcgaacttga ctacaggttt gccaggcacc
      781 aacccatcca tcgccttggt gatatccgcg gtaaaaatgt cccggcgata ttcgccgtcc
      841 gtaaagacat cgacaccgct ggccttttgc atcgccaaag cctggaggat ggaccggtcc
      901 tcgagctcgc gcacctgggg ctcatccatg cgcccctcgc gcagcgctgc ccgcgcttca
      961 agcaactccg ccggccgcaa cagactgccg atattatcgg cccgaaatgg catcgccata
     1021 aatagactcc cttccgaagt tgaactggtg aacagaacgg tatcgttcga caattcatag
     1081 gttcaaccgt tcaacctccg aatccgaaag caaccttgaa cttatgaacc gttgaagctt
     1141 gaacgaaatt atttttccca cacctgccgc gccgtctcga cgaccacttc caactttctt
     1201 ttttgatcgt cttcgctgac cagattgccg accacgtccg aggcaaagcc gcattggggg
     1261 ctgatcgcca gttgttcgag cggtaaatac ttagccgcgg cgtcgatccg gcgcttgaga
     1321 tcgtcgacgg tttcgatctc cggcaccttg gtgctcacca gtccgagcac caccactttg
     1381 ttcttcggca cgaagcgcag cggcgcaaag gtgcccgccc gtgggctgtc atactcaagt
     1441 aaaaaccgat catgacctag ctcggtaaag agcatctcgg cgatggcgtc gtaactgccc
     1501 tcgcgatgcc acatgctgcg ctggttgccg cggcagaggt gtatgccgaa ggtcacgtcg
     1561 tcgaagagcc gcaagatctc gttatcggca ttgaccgagc gcatgagatt tttcaccggg
     1621 tcctcaccgc gcgccttcat ctcatcgagc gacggcgcgt cgacataggc cgtataactg
     1681 ggcgcatcga tttgcacgta acggcaaccg gcgtcgacca ggccgcgaat catttcccgt
     1741 tcgatgtgcg cgacatcggc gatgaagtct tccatgctcc agtaaatcga ttttgagttc
     1801 tgataatcga aacgctgcga tattcggtct gggccgacca gggtgacctt ggccggccgc
     1861 tgggcgacac tgctgacaaa caaatattct tccaacggta aatttcgctg caaacgcagc
     1921 ttttgcgata ccggccggcg ctgcaatatc gccgccccgt cggcgacatg ataagggtcc
     1981 cagcgctgca gcggcttccc ctcgcgcgac agactttcga caaacgacag gtccgccggg
     2041 cgggcatcga agccgctcac cgatgcggcg aagctatcct ggaaattaag ccggcggaac
     2101 tcgccgtcgg tgacgacttc caggccgatc gcctcctgca gccgcaccgc atggccgatc
     2161 gcttcgtcct cgattcggcg caattcctcg gcggtgattt ttcccgcatc ttgatctgcg
     2221 taggcctgtt tgagatggct ggggcgaagt aaactgccga caacgtcggt cctgatctgc
     2281 gctaagcgct tcatcgctgt ttgaaccatg acaatactcc cgcattaatt aaaagattag
     2341 aactcctaaa gcggctcaga ctccggctcg catcctcaac gacgccaacg actccgacgc
     2401 ctcgcggaca accttctcca catccagggc ggtcatctga ccgccacgtt ttacaattcg
     2461 accgtcgacg accacggtat cgacgttgtg cggctgcgcc gagcgcacga tcgcttgcac
     2521 cggatcgccc agaggcgcca tgttcaggtc ggtggcgcgc accaagatta aatccgctcg
     2581 cttgccgggt gtgagcgacc cgacgcggtc ggcaatatcc agatcgcgcg cgccattgat
     2641 ggttgccaac tccagaacct gccgcgccga caagcttgtt gggtttttgt taatgacgaa
     2701 ctgggtgtcg atgagcgcat gcatgtgggc aaacatatcg ttgttccccg atatcgccgc
     2761 ggtatcgatc gacatactca ccaggatgcc gtgcttgagc atgtcaacca accgggacga
     2821 cacaaacgac gagcgcattt cggcaaatgg actcaagcaa acatgcgctc tagctttaac
     2881 aatgcgcgcg aaatcggcct cgtcataggc ggtaggatta atcagctgga catcagggcc
     2941 gagcattgct tcgcgttcca gcaacgcgac gcgccccgct ccgccagtat gaatcgtaat
     3001 cggcagcccg agcttgcgtg cgccgtccca atcgcggcga agcgtcggcg tcgtcaccgc
     3061 gccgcgcggc gaatcgctga gactgcgggc agccatgccg agggtaagca gacggtcgct
     3121 cggcccactg aaccattgtc gttgcacgcg cgccaggtcg accagatcca tcgtctgatc
     3181 ggcgggaagt ccgccttgag gc
//
LOCUS       DASQKS010000327         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1842915, whole genome shotgun sequence.
ACCESSION   DASQKS010000327 DASQKS010000000
VERSION     DASQKS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842915"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VE170_08535"
     CDS             complement(<1..732)
                     /locus_tag="VE170_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15522.1"
                     /translation="MPTDICYWKHDIDPDEAFLCYDGKSKVCSSCCVELCPKETPQWF
                     AACARAGHPTWPSVARGRRVPSKLVVLESYWDSRLFQTKTVKGFFEAMGPLHDPPLQL
                     AHRFVESQRGLAHYTAAPDGLLWRDPGAADAPIFYLAFHGAPSEVKSVLDSIGEQALC
                     DGFKGFGVKPNLVYFAACSVLRDQKGRQFAKKFLDASGCRAVLGYTTDVNWMLSLVID
                     MLFLHRFYCNEDPWKNLRKIFESVLS"
     gene            complement(759..1544)
                     /locus_tag="VE170_08540"
     CDS             complement(759..1544)
                     /locus_tag="VE170_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ15523.1"
                     /translation="MKWLGTAGWEMRIGATTILIDPFVTRREAADDVEWKTNNDEVSK
                     FITKADFIFAGHSHADHIADIPVIAKRFGAKVFGSRTTINIALSAGVDKSQLTTISGG
                     DKLDFKDFSVQVIESRHGVLNRGGRRRQGKFAEVLRPWTGPIMGDAFVEGGCYLYYFT
                     FGHLRVLHQSTGNFIEDNLKGLQPDVAILADNSNYDWTNALNILRPKTVIIHHYDEWR
                     TPFADGIPESNRRRAQRFERLIKSVDARIKVIIPELLKPVVWE"
     gene            complement(1672..2142)
                     /locus_tag="VE170_08545"
     CDS             complement(1672..2142)
                     /locus_tag="VE170_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ15524.1"
                     /translation="MKLAIATTINGQREEIAVSPHQTLLEFLRDELKLKGAVEGCSVG
                     VCGSCTVLIDGAPVSSCLTLASNIEGKAVTTIEGLSMSGELDPVQQAFLKNQAFQCGF
                     CTPGMIMAVKGLLNAYAHPSEEQARDYLSGNLCRCGTYKEVLAAVRELTAARAK"
     gene            complement(2144..>3875)
                     /locus_tag="VE170_08550"
     CDS             complement(2144..>3875)
                     /locus_tag="VE170_08550"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ15525.1"
                     /translation="VTALWEPSGKLVVYTPSQTPSPLQEQIAKVFKMPLNRVRVVVPF
                     IGGGYGGKNHARIEPVVALLARKARRPVQWILTRDEVFFTGRRFGAVVKIKTGFKRDG
                     RLWARKVDAWYDMGAYALSGPANTKNAAIIAGGPYNIPNRDFTTYAVYTNLPPAGPYR
                     GVGASHVCWAYESDLDDIARRCNLDPLEIRLKNLIQENDRFITGEPMISVGVSECLKQ
                     VAQSINWQIGAEQSLRNDATAAEQSKHSDSIVRGKGLAVAIKSTSTPSTSAASVRLNA
                     DGSAILLTSTSDMGQGAQTALAQIVADELALPFERVTVTFPDSDITPYDKSTSSSRAT
                     FHMGKAAQIAVGQIREQLFHAAAKALEARVEDLELRDSRLQVKGVPERSLTYPQLFKA
                     IYGEASGNLFGSHTLRTEGGVDAVTGQGKGSSFWFYSAAGAEVEVDTETGKVRVLQVA
                     SAVDVGKAVHPLQCSLQNEGSALAGLGSALFEEMRYDSGQPINSTFLDYLLPSMLDHP
                     GEFKSLLVETPHPDGPCGAKGMGEAALPPMAPAIGNAIANALHGMRVRDLPVRPDKII
                     AVLNSSKEKR"
BASE COUNT          743 a         1112 c         1194 g          826 t
ORIGIN      
        1 actcagcacc gactcgaaga tcttgcgcaa gtttttccac gggtcttcgt tgcagtagaa
       61 gcggtgcagg aacagcatgt cgatgaccaa gctgagcatc cagttgacgt cggtggtata
      121 accgagcacg gcgcggcagc cggaggcgtc gagaaacttc ttcgcgaact gccggccttt
      181 ttgatcgcgc aggacgctgc acgcggcgaa gtagactagg ttcggcttaa ccccgaaacc
      241 tttaaaaccg tcgcacagcg cctgttcgcc gatggaatcc aagaccgact taacttcact
      301 gggcgcgccg tgaaacgcga gatagaaaat tggcgcgtcg gccgcgccgg gatcacgcca
      361 caatagaccg tccggcgccg ccgtgtaatg tgccagtccg cgctgggact cgacaaagcg
      421 gtgggcgagt tggagcggcg gatcgtgcag cggtcccatg gcctcgaaga agcctttgac
      481 ggtcttggtt tggaacagtc ggctgtccca ataggactcc aaaaccacca gctttgacgg
      541 cacccgccgg ccgcgggcga cgctcggcca agtcggatgg ccggcccgag cacaggcggc
      601 gaaccattgc ggcgtctcct ttggacaaag ctcgacgcag caactggagc acaccttgct
      661 cttaccgtca tagcaaagga acgcttcatc tgggtcgatg tcgtgtttcc agtagcagat
      721 gtccgtaggc atggggggat agagtacttg ggtgcgattt attcccacac taccggttta
      781 agcagttcag ggatgatgac tttgattctc gcatcgaccg atttgatcag tcgctcgaag
      841 cgttgggctc tgcgcctatt cgactcggga atgccatctg caaagggcgt gcgccattca
      901 tcatagtggt ggatgattac cgttttaggg cgcaggatat taagcgcatt cgtccagtcg
      961 taattgctgt tgtcggcgag gattgccaca tccggctgca gtcccttcaa gttgtcctcg
     1021 atgaaattgc cggtgctttg atgcagcaca cgcaagtggc cgaaggtgaa ataatatagg
     1081 taacaaccac cctcaacgaa agcatcgccc atgatcgggc cggtccacgg gcgcaagact
     1141 tctgcgaatt tgccttggcg gcggcgcccg ccgcggttga gcacgccatg ccggctttcg
     1201 atcacctgga ccgagaagtc tttaaagtcg agcttgtcgc cgccgctgat cgtcgtcagt
     1261 tgactcttat cgactcccgc gctcagggca atattgatcg tggttctgga gccgaatacc
     1321 ttggcgccga agcgcttggc aattaccggg atatcggcga tgtggtcggc gtggctgtgg
     1381 ccggcgaaaa tgaagtcggc cttggtaatg aatttggaaa cttcatcgtt attagttttc
     1441 cactcgacgt catcagctgc ctcgcgcctg gtcacaaatg ggtcgatgag aatcgtcgtg
     1501 gcgccgatcc gcatttccca gccggccgtg cccagccatt tcatcgcgac ggcatctgcc
     1561 gctcgcgttt ggtctagctc ccaagttatt tggccgagca gagaacatac gaccaggagc
     1621 agcagtgaac gacaacccct catagtcctc ttcgcaccgg tcgaaagtaa actacttggc
     1681 tctggctgcg gtcagttctc gcaccgccgc caggacttcc ttgtaggtac cgcagcggca
     1741 caggttaccg gagagataat cgcgcgcctg ttcttcgctc gggtgggcat aggcgttcaa
     1801 tagtcctttt accgccatga tcatgcccgg ggtgcaaaag ccgcattgaa aggcctggtt
     1861 cttcaagaag gcctgctgga ctggatcgag ctcgccactc attgacaagc cttcgatggt
     1921 agtgactgct ttaccttcga tgttgctggc gagggtaaga caggaactca ccggtgcgcc
     1981 gtcgatcagc accgtgcacg aaccgcagac gccgacgctg caaccttcga cggcgccttt
     2041 gagtttaagc tcgtcgcgca ggaattccag cagagtctga tgggggctga cggcgatctc
     2101 ttcgcgctgg ccgttgatcg tggtggcgat ggcgagcttc atcttagcgc ttttccttgc
     2161 tggagtttaa tacggcgatg atcttgtccg gccgcaccgg caagtcgcgc acgcgcatgc
     2221 cgtgtagggc gttggcgatg gcattgccaa tggctggtgc catcggcggc aaagccgctt
     2281 cgcccatgcc cttggcgccg catgggccgt cgggatgggg cgtctccacg agcagcgatt
     2341 tgaactcgcc cggatggtcg agcatcgacg gcaggagata gtccaggaac gtgctgttga
     2401 tcggttggcc gctgtcgtag cgcatttctt cgaacagcgc cgagcccaag ccggcgagcg
     2461 cggagccttc attttgcagg ctgcactgga gcgggtggac cgccttgccg acatcgacgg
     2521 cgctggcgac ttgcaagacg cgcactttgc cggtttcggt gtcgacttcg acctcggcgc
     2581 cggcggcgga gtagaaccaa aacgacgaac ccttgccttg gccggtcacg gcgtcgacgc
     2641 cgccttcggt gcggagcgtg tggctgccga acaagttgcc gctggcctcg ccgtagattg
     2701 ccttgaagag ctggggatag gtcaggcttc tttccggcac gcccttcact tgcaagcggc
     2761 tgtcgcgcag ctctaaatcc tcgacacgcg cttcgagcgc tttcgcagcc gcgtgaaaca
     2821 attgctcgcg aatctgtccg acagcgatct gtgcggcttt gcccatgtga aacgtcgcac
     2881 ggcttgagct tgtcgattta tcgtagggag ttatatcgct gtcgggaaat gtcacggtca
     2941 cacgctcgaa aggcagagcc aattcgtcgg cgacgatctg cgccagcgcg gtctgggcgc
     3001 cctggcccat gtcggaggtg ctggtgagca agatcgccga gccgtcggcg ttcaaacgca
     3061 cgctggcggc ggaggtgctc ggcgtcgagg tactcttgat cgccaccgcg agccccttgc
     3121 cgcggacgat tgaatcgctg tgtttcgatt gctccgctgc cgttgcatcg ttgcgcaagg
     3181 attgctccgc tccaatttgc caattgatgg attgagccac ttgtttgagg cattcggaga
     3241 cgccgacgga aatcatcggt tcgccggtga taaaacggtc gttttcttga atcaaatttt
     3301 tcagccggat ttcgagcgga tcgagattac agcgacgcgc gatgtcgtcc aggtccgatt
     3361 cataggccca gcagacgtgg gaggcgccga cgccgcgata ggggccggcc ggcggcaggt
     3421 tggtgtaaac cgcgtaggtg gtgaaatcac gattaggaat gttatagggt ccgccggcga
     3481 tgatggcggc gtttttggta ttggccggac cggagagcgc gtaggcgccc atgtcgtacc
     3541 aggcgtcgac tttgcgcgcc cacaagcggc cgtcgcgttt gaagccggtt ttgattttta
     3601 ccaccgcgcc gaagcgccgg ccggtgaaaa aaacttcgtc gcgggtaaga atccactgca
     3661 ccgggcggcg cgccttgcgc gccagcagcg cgactacagg ttcgatgcgc gcgtgattct
     3721 tgccgccgta accgccgcca atgaacggca cgacgacacg cacgcggtta agcggcattt
     3781 tgaaaacctt ggcgatctgc tcctgcaggg gcgacggcgt ctggctcggc gtatagacca
     3841 ccagcttacc gctcggctcc cacaaagcgg tgacc
//
LOCUS       DASQKS010000328         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3951058, whole genome shotgun sequence.
ACCESSION   DASQKS010000328 DASQKS010000000
VERSION     DASQKS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951058"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VE170_08555"
     CDS             complement(<1..1241)
                     /locus_tag="VE170_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYJ15526.1"
                     /translation="MRAHRRQLLPLFQKNEVLFGHDVTPAAIAYAVDSGASVKIFFRD
                     GVTVRSEAATLQPFMLLRGDETLSGWGGEGQIENLAGGGAFNRLALFADLKQLDDAKA
                     FLQKKTGKTPSAGDAPYWYFSDPLHQFLLLSGRTHFLGMTFRDLHRLQIAIETYCQSS
                     FNFPSAAREHDRITAIALSDSTGWERLISGKEYGEAEMLHELVQEIQRRDPDVIEGHN
                     IFRFDLEYLEARARRHKVALTFGRDGSRLSGHASRMQMAERTITYRKYEIFGRHIIDT
                     WILAQHYDVATRELETFGLTDIARHFGVAGAERAYVPGDKASWYFDHDADTLFRCALD
                     DVRETRAIAEILSPSYFVQAQIFPFSYQNMPLRGNATKIDTLFLREYLHQRHSVPFPD
                     EVGAVIGGFTDMEYQGTANHVL"
     gene            1377..2156
                     /locus_tag="VE170_08560"
     CDS             1377..2156
                     /locus_tag="VE170_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HYJ15527.1"
                     /translation="MDPLSFDNRPALRRPILVLAFAGWSDAGASATTALRYLVDNLMG
                     KKFASIDPEEFYDFYRQRPVVRLNDAKVREIHWPSFDFYHGAGIGLEKDFIFGIGAEP
                     HLRWRTFTETMLRFARECETEMVVTLGAYLDEVLYTRPVPLTGFSSDAKLMERLDLAP
                     SRYQGPTGIVGVLTDACRQSGIENLSIWASLPHYLNVSPNPRGSLALLLRMNQWLGLR
                     VDTTPMERAAADFQAKINEAVNADPKLSAYVRELKKREFEQ"
     gene            2153..2863
                     /locus_tag="VE170_08565"
     CDS             2153..2863
                     /locus_tag="VE170_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ15528.1"
                     /translation="MKLSVLVPVYNEERTLEEVVRRVCAFPKAKEIILVDDGSKDRSR
                     EILTRLQAANQLAQDPLNHIRIFFQERNQGKGAALKTALGHATGEIILVQDADLEYDP
                     ADYPALIEPIEKGLADVVYGTRFAGGGAHRVFFFWHSLGNRILTLLSNMLSNLNLSDM
                     EVGYKVFRAQVLEGIELKSRRFGFEPEITMKLAKKRCRFYEVPISYHGRTYEEGKKIT
                     WKDGIAALYYMLLFRFSD"
     gene            complement(2915..3874)
                     /locus_tag="VE170_08570"
     CDS             complement(2915..3874)
                     /locus_tag="VE170_08570"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15529.1"
                     /translation="MNILITGAGLIGAHAARQMVDAGHKVVLFDLSPNRDYVANVVGH
                     GKADVVAANMLDLPALLTALEKFDVDTMVHTAGLIGGRVQENSYTGATNNILGTINIL
                     EAARLRKLRRVVYVSTFGVYDRGKINDSVVKEVHSIGGHNLYATTKVCSEHLVHAYTS
                     MYNLDTIIIRPGGVFGRGFYVGGSTVGMVMRDLALAMIKGEPITIEAKTYGPNEYVYG
                     KDVGLALLLACQARNPKQRIYNAGTGAVHGAEQLAQVMREVAPKLEVKVAGSSAADKS
                     RSIPMDISVSQAELGYTPKFPLKEALKDYMDELWNEGARVTTN"
     gene            complement(3912..4883)
                     /locus_tag="VE170_08575"
     CDS             complement(3912..4883)
                     /locus_tag="VE170_08575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15530.1"
                     /translation="MKLTVAILAWLALICIGATAHGQAPLERIVITYPSRSIASVDLY
                     IAQERGFFRQEGLIADVVQVRGNIGVTALLSGDAHAINNVGTLIRAMERSDLPAKVMS
                     QSLKKNLFWLVTKPEIKSLAELKGKTFGTTTFGGSQHMAGLRLLQKAGLDTDKDITMV
                     IGGDVPAQLQSLMSGVIHLAALSPPTVILARDKFKLRVHGSTLDDLPNLQNGLAFSEK
                     LLREKRDLVKRILRARSRAHRYLFENERGSAEVLAKLLSVDLNVALESYRLARPAFTT
                     NGLPTDKEVEEFLRADAEVLKLKEPVPAAKIFDFSLQREVNQELGIK"
     gene            5006..5425
                     /locus_tag="VE170_08580"
     CDS             5006..5425
                     /locus_tag="VE170_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15531.1"
                     /translation="MKVKQIYHISLPCNDLERGVKFYTDVLGMKCTKIGYDTESGGPY
                     KEKYGMFPNIARLFMEDGMCLVLFQRPEEVERGDFDEGTSHLALEVSPEDFEKATDEL
                     TKAGVKVVIDKPVVRPTGKAIYFFDTEQNYIQLWSRP"
BASE COUNT         1154 a         1484 c         1646 g         1241 t
ORIGIN      
        1 agcacatgat tggccgtgcc ctgatattcc atatcggtga aaccgccgat gacggcgccg
       61 acctcgtcag ggaagggcac ggaatggcgc tgatggagat attcgcgcag gaacagagtg
      121 tcgattttgg tggcattgcc gcgcagcggc atattctggt agctgaacgg gaaaatctgc
      181 gcctgaacga aatagctggg cgaaaggatc tcggcgatcg cccgggtctc gcgaacatcg
      241 tcgagggcgc agcgaaacaa ggtgtcggca tcatgatcga aataccagct ggccttgtcg
      301 cccggcacat aggcgcgctc ggcgccggcg acaccaaaat ggcgcgcaat gtctgtcaag
      361 ccgaaggtct ccaactcgcg ggtggcgaca tcgtaatgtt gcgcgagaat ccaggtgtcg
      421 atgatatggc ggccgaagat ttcatatttt cgataggtaa tcgtccgctc ggccatctgc
      481 atgcgcgagg catggccgct caagcgtgag ccgtcgcggc cgaacgtgag cgcgactttg
      541 tggcgcctgg cgcgggcctc gagatattcc aaatcgaagc ggaaaatatt atgtccctcg
      601 atcacgtccg ggtcgcgccg ttgaatttct tgaaccagct cgtggagcat ttcggcttcg
      661 ccgtattctt tgcccgagat gagccgctcc caaccggtag aatcggacag cgcgatggca
      721 gtgatacggt cgtgctcccg cgccgcactg ggaaaattaa aactgctctg gcaataggtt
      781 tcgatggcga tctgtaagcg gtgcaaatcg cgaaaggtca tgccgagaaa atgcgttctg
      841 ccggaaagta aaagaaactg atgcagcggg tcactgaaat accaataggg cgcgtcgccg
      901 gcggagggcg tcttgccggt ctttttctgc aaaaaagcct tggcatcgtc gagctgtttc
      961 aaatcggcga acagcgccaa tcgattgaag gcgccgccgc cggcgagatt ttcaatttgc
     1021 ccttcgccac cccagccgct aagagtttcg tcgcctcgga gcagcatgaa cggctgtaaa
     1081 gttgccgctt cactgcgcac cgtcacgccg tcgcgaaaaa aaatcttgac gctggcgccg
     1141 ctatccaccg cgtaagcgat cgccgccggg gtcacgtcat ggccaaacag gacttcgttt
     1201 ttttgaaata gcgggagtag ttgtcgccgg tgtgcgcgca taagttcagc gccgactata
     1261 gcaaacggct cggtcttctc ctagccgcgg ccgcggcgat gccaataaaa ttattggtct
     1321 taatcccgct tttccctgga gggtatgggg cgggatatgg taaattaacg gcaattatgg
     1381 acccgctcag ttttgacaac cgcccggcgc tgcgcaggcc cattttggtg ctggcatttg
     1441 ccggctggag cgatgccgga gcatcggcga ccacggcgtt gcgttatctg gtggacaacc
     1501 tgatgggcaa gaagttcgcc agcatcgatc ccgaagagtt ttacgatttc taccgccagc
     1561 ggccggtggt gcggctcaat gacgcgaagg ttcgcgagat ccattggccg tcattcgatt
     1621 tttaccacgg cgccggcatt ggtctggaaa aggattttat attcggcatc ggcgccgaac
     1681 cgcacctgcg ttggcgcact tttactgaaa ccatgctgcg ttttgctcgc gagtgcgaga
     1741 cggagatggt agtgactttg ggcgcctatc tcgacgaagt gctctacacc cggccagtgc
     1801 cgctcaccgg tttttccagc gatgctaaac tgatggagcg gttggatctc gcgccgtcgc
     1861 gctaccaagg ccccaccggc attgtcggcg tattgacgga tgcttgccgc cagagcggta
     1921 tcgagaatct cagcatttgg gcgtcgctgc cgcattatct caatgtttcg cctaatccgc
     1981 gcggcagctt ggcgctgctc ttgaggatga accaatggct cggcttgcgt gtcgacacga
     2041 cgccgatgga acgcgccgct gccgattttc aggcgaaaat caacgaagcc gtcaacgccg
     2101 acccgaaact ctccgcttac gtgcgtgaat tgaagaagcg cgaattcgaa cagtgaaact
     2161 ctccgtcctg gttcccgtct acaacgaaga gcgcacgctg gaggaggtcg tacgccgagt
     2221 gtgcgcgttt ccgaaggcga aggagatcat tcttgtcgac gatggttcca aggatcggag
     2281 ccgtgagatt ctcacgcgtt tgcaggcagc gaaccagctg gcgcaggatc cactcaatca
     2341 cattaggatt tttttccagg agcggaacca gggcaagggt gcggcgctca aaaccgccct
     2401 cggtcatgcg accggcgaaa tcatactagt tcaggatgcc gacctcgaat atgatccggc
     2461 ggactatccc gcgctgatcg agccgattga aaaaggactg gccgatgtgg tctatggcac
     2521 tcgtttcgcg ggcggaggcg cgcaccgggt gttctttttt tggcactcgt tgggcaatcg
     2581 cattctgaca ttgctgtcca acatgctttc caatctgaat ctctccgaca tggaagtcgg
     2641 ttacaaagta tttcgcgcgc aagtgctgga aggcatcgaa ctaaaatcgc ggcgtttcgg
     2701 cttcgaaccc gaaatcacca tgaagctcgc caagaagcgt tgccgcttct acgaagtgcc
     2761 gatctcctac cacgggcgca cctacgaaga gggcaagaaa atcacctgga aagacggtat
     2821 tgccgccttg tattacatgc tgctgttcag attctccgat tgatcgtcgc ggtaaaaagc
     2881 ggccgcgtca tgcggcgcgg caacagatcg ttggttaatt tgttgttact ctcgcgccct
     2941 cgttccacaa ttcatccatg tagtccttga gcgcttcctt gagcgggaac ttgggcgtat
     3001 aaccgagttc ggcttgactg acgctgatat ccatcggaat gctgcggctc ttgtcggcgg
     3061 cgctgctgcc ggcgactttc acttccagtt tcggggcgac ttcgcgcatt acttgagcta
     3121 attgttcggc gccgtgcacc gcgccggtgc cggcgttgta gatgcgctgc tttggattcc
     3181 gcgcctggca agcgagtagc aatgcgagac cgacatcctt gccgtagaca tattcgtttg
     3241 ggccgtaagt cttggcttcg atcgtgatcg gctcgccttt gatcatggcc agcgctaggt
     3301 cacgcataac catccccacg gtggagccgc cgacgtagaa gccgcggccg aacacgccgc
     3361 cgggacggat aatgatggtg tccaagttgt acatgctggt gtaagcatgg accaggtgct
     3421 cagagcaaac tttggtcgtg gcgtaaaggt tgtggccgcc gatggaatga acttctttga
     3481 ccacgctgtc attgatcttg ccgcgatcgt agacgccgaa ggtgctgacg tagaccaccc
     3541 ggcgcagttt tcttagccgc gccgcttcga gaatattgat cgtaccgaga atattattag
     3601 tggcgccggt gtaagagttt tcttgcaccc tcccgccgat caaacccgcg gtgtggacca
     3661 tggtgtcgac gtcgaatttc tccagcgccg tgagcagcgc cggcagatcg agcatgttgg
     3721 ccgcgacgac atcggcttta ccgtggccga ccacgttggc gacgtaatcg cgattgggcg
     3781 agagatcgaa aagcacgact ttgtggccgg cgtcaaccat ttgccgcgcc gcgtgggcgc
     3841 caatcagtcc ggcgccggtg atgagaatgt tcatgctgcc tcctagtagg ttcttcttga
     3901 tagggttttt gttacttgat acctaattct tgattcactt cccgctgcag gctgaagtcg
     3961 aatatcttcg ctgcgggcac cggttctttg agcttcagca cttcggcgtc ggcgcggaga
     4021 aattcttcga cttccttgtc ggtgggcagg ccgttggtgg taaaggccgg acgggccagc
     4081 cgataggatt cgagcgcgac attcaaatcg acactcaaga gcttggccag gacttcggcg
     4141 ctgccgcgtt cattctcgaa taaatagcgg tgtgcgcgcg agcgggcgcg caggatgcgt
     4201 ttcaccaaat cgcgtttttc gcgcagcagt ttttccgaga aggccaagcc gttctgcaga
     4261 ttaggcagat cgtccagggt gctgccatgg acgcgcaact tgaacttgtc gcgcgccaga
     4321 atgacggttg gcggtgacag cgcggccaag tgaattaccc cgctcatcag gctctggagc
     4381 tgggcgggca cgtcgccgcc gatcaccatg gtaatatctt tgtcggtatc gagcccggct
     4441 ttttgcagca ggcgaagacc cgccatatgc tgcgagccgc cgaaggtggt ggtgccgaag
     4501 gttttgcctt tgagttcggc caagcttttg atttccggtt tggttaccaa ccagaataga
     4561 ttcttcttga gactttgcga catgactttg gccggcagat cgctgcgctc catggcgcgt
     4621 atgagcgtgc cgacattgtt gatcgcgtgg gcgtcgccgc tgagcagggc ggtgacgccg
     4681 atattgccgc gtacctggac gacatcggcg atgagtcctt cctggcggaa gaagccgcgt
     4741 tcctgggcga tatagagatc gacggaggcg atgctccggc tggggtaggt gatgacgatg
     4801 cgctcgagcg gcgcttggcc gtgggcggtc gcgccgatgc agatcaaggc caaccaagcg
     4861 agaatagcga ctgttagttt catatggctc ccgatactta gtggaaatgt catggatgtc
     4921 aaccgcggcc tggcgtggct caaccctctc ttgaaaccgc gctggaactt acagtataca
     4981 agggtaacca aggaggttaa ttgcgatgaa agtaaagcaa atctatcaca tcagcttgcc
     5041 gtgcaacgac ctggagcgcg gcgtcaagtt ctataccgat gtcttaggta tgaagtgcac
     5101 caagatcggt tacgataccg agagcggcgg cccatacaaa gaaaaatacg gcatgtttcc
     5161 gaacatcgcc cggctgttta tggaagatgg catgtgtctt gtgctgttcc aacggccgga
     5221 agaggtggag cggggcgatt tcgatgaggg cacttctcat ctcgcgctgg aagtgagccc
     5281 ggaagacttc gaaaaagcta ctgacgagct gaccaaagca ggtgtcaaag tcgtgatcga
     5341 caagccggtg gtccgaccga ccggtaaggc gatctatttc ttcgataccg agcagaatta
     5401 cattcagctg tggtcgagac cgtaattgtg accgtaagcg gcgggtagtc aaaactcgtt
     5461 tcgcatcgcc aaagttttaa atatccattc cccgagtttc ctgaagaatt ttttcagggg
     5521 gctca
//
LOCUS       DASQKS010000329         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_168082, whole genome shotgun sequence.
ACCESSION   DASQKS010000329 DASQKS010000000
VERSION     DASQKS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168082"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1111)
                     /locus_tag="VE170_08585"
     CDS             complement(131..1111)
                     /locus_tag="VE170_08585"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15532.1"
                     /translation="MKKIILSMIPPLLVVVALLAGKSVFALDRVNVTLPSKSFQFIIF
                     PIAKERGYMKEEGIDLNTTIMASTPGLQAVLAGEMDFTGSGSSALVAVAQGNAPLKTV
                     LAVTDQVLQWLMVRPQYGSFKELRNKKIAVTGVAAVATFMLKKVAPKYGLDANKDLTF
                     LALPPGQRLAALQTGVVDGGLLSSEERFAALDHGMKEILYLGKEVKNSWGTIATNDQF
                     IKVKPKLMQGFTRALLKALRLVKQNREVAMDAMIKFSELDRELAARTYDSMIGTFTTN
                     GVVDEETQKNDLDIVREVLKVTKEVPIERAYDFSFAKKADSELTQAGWKP"
     gene            complement(1448..2239)
                     /locus_tag="VE170_08590"
     CDS             complement(1448..2239)
                     /locus_tag="VE170_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15533.1"
                     /translation="MIGESTTQMRLCPYCANSIAENAADCPYCKADLSSQFTPPQWLK
                     RDGQSGQTKLGRENHKRFSIPAKLIWITAMLVVALTAFFAGGYKQRSELSLSSQANLK
                     QLQGKDQMIQSQEGQLAQSRQQLNDATNQLAEMKTKFEASEKELLAAKQRVSAAAHPI
                     EGPATSRAPVARRTAARATDTANSQAQPATARQTTAPAVYETTRATTVYENPSSAARA
                     ISQIGGGTRINVVGSASGWLEVRSKRGNPPGYVRSDDARQIGGTS"
     gene            complement(2400..>3106)
                     /locus_tag="VE170_08595"
     CDS             complement(2400..>3106)
                     /locus_tag="VE170_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HYJ15534.1"
                     /translation="GHRIVGLIAQAHLTADARREIERILPAGMTLADTAVWPDHEGRQ
                     IRDFDPLHYVRIPERASGYDQARDCPDRNCMVEALNWFSAAVADKNAPIVTRRLALRY
                     VAHLVGDMHQPLHTGRAADRGGVDISVSYKGTTTNLHYFWDANLVDMETGNEEEIAKR
                     LTATITAADRLKWQAGDPKQWTDESLMLVRSHAYNTGASGELTDDYVEKARPVVRRKL
                     AQAGIRLAWLLNIALK"
BASE COUNT          628 a          864 c          872 g          742 t
ORIGIN      
        1 cttttcagat attaactcaa ttgcaaaagg ttcggcatat ggcgacaccg gcggccattg
       61 ggcggcgatc ggaacgatca agtaatttcc cacttttgcc gaggctcggg aagcccatag
      121 gcgagaattc ctaaggtttc cagcctgctt gcgtcaattc gctgtcggct ttcttggcaa
      181 agctgaaatc gtaggcgcgc tcgatgggca cttcttttgt gactttaagg acttcgcgaa
      241 cgatatcgag atcgtttttc tgggtttctt cgtcgaccac gccgttggtc gtaaaagttc
      301 caatcatgct gtcgtaagtg cgcgccgcca attcccggtc taactcggag aactttatca
      361 tcgcatccat cgcaacctcg cggttctgct taaccaggcg cagggctttg agcaaggcgc
      421 gcgtgaatcc ctgcatcaac ttgggtttca ctttgatgaa ttggtcattg gttgcaatgg
      481 tgccccagga gtttttcact tccttgccca gatacaaaat ctctttcatg ccgtgatcga
      541 gggcggcaaa gcgttcctcc gaactcaaga gccccccatc cacgaccccc gtctgcagtg
      601 ccgcgagacg ctggcccggc ggcagcgcga gaaaagtcaa atccttgttg gcgtcgaggc
      661 cgtacttcgg cgcaaccttt ttcaacatga acgtcgctac cgcagcaacg ccggtcacgg
      721 cgatcttttt gtttctgagt tccttaaaag agccgtactg ggggcggacc atcagccact
      781 gcagcacctg atcggtgacg gccaagacgg tttttagcgg cgcattgcct tgagcgacgg
      841 cgaccagcgc gctgctgccg gaaccggtaa aatccatttc gccggcgagc acggcttgca
      901 gaccgggggt ggatgccatg atcgtagtgt tgagatcgat cccctcctct ttcatataac
      961 cacgttcctt ggcgatcggg aaaataatga actgaaacga cttcgacggc agggtgacgt
     1021 tgacccgatc aagggcgaat accgatttac cggcaagcaa cgccacaaca accaacagcg
     1081 gcgggatcat cgacaggatt atttttttca tcgttcagtc cctcctgaaa aataacatgc
     1141 cgggcacgcg gcaacaccgg tcccgcccac attataaaag tcgattttcg atgtcgaggg
     1201 gaatttaacc gtttacgaat ggctatgacg gtcgctgacg atgaagggtg tgaagttaaa
     1261 aggtgatttc cgcagaaaaa acaacccttg gcgcgacaga ccgacactca cccaaacgga
     1321 gggttccggc ttgctttcaa aacacaatcc gctggaatgt cgcattggca gtagtcgaac
     1381 cgatcgtcgc ccggcggatt taagcttcaa cagtcaatca agaacggggc gggggggccg
     1441 gcaattttca gctagttccg ccaatctgcc gcgcatcgtc ggaccgcaca tatcccggag
     1501 gattgccgcg cttggaccgc acttccagcc aaccgctggc agaacctacg acgttgatcc
     1561 tggtgccgcc gccgatttgc gagatagccc gcgcggccga cgacgggttt tcatatactg
     1621 tcgtcgcgcg agtggtctcg tagacagccg gtgcggttgt ttgtctcgcc gtcgccggtt
     1681 gagcttgcga gttagcggtg tccgtcgccc gtgctgccgt tcttcgagcg accggcgcgc
     1741 ggctcgtggc cggaccttct atggggtgcg cggcagcact cacgcgttgc tttgccgcca
     1801 agagttcttt ctcacttgct tcgaatttcg tcttcatttc ggcaagctgg ttggtggcat
     1861 cgttcaattg ttggcgcgat tgagccagtt gcccttcctg actttgaatc atttggtctt
     1921 tgccttggag ctgcttgagg ttggcctgcg acgacagcga gagctcgctg cgttgtttgt
     1981 agccgccggc aaaaaaggcc gtcaaagcca caacgagcat ggcggtgatc cagatcaatt
     2041 ttgccgggat tgaaaatctc ttgtgattct cccggcctaa ttttgtttgc cctgattgcc
     2101 catcgcgctt gagccattga ggaggagtaa actgcgatga gagatcggcc ttgcaataag
     2161 ggcaatccgc ggcgttttcc gcgatactat tggcgcagta tgggcaaagc cgcatttgag
     2221 tggtggattc tccgatcatg accattacct cctaagtcac aggcgccaac cgtgtccacg
     2281 catttgccgg ggaccggggc ggattctgaa aacagcccag cggcgtcagg cgccatccct
     2341 ttagacgttt ggcaaccgca attgtgaatg gtcctaggtc gttcggtatt cgggggttgc
     2401 tacttcaacg cgatatttag caaccaagcc aacctaattc cggcctgagc aagttttctg
     2461 cggacgaccg gccgggcttt ttccacgtaa tcatcggtta gttcacctga agcgccggtg
     2521 ttgtatgcgt gcgacctgac taacatcaaa gattcatcgg tccactgttt gggatcgccg
     2581 gcctgccatt tgaggcgatc cgctgccgtg atggtcgctg taagccgctt ggcgatttcc
     2641 tcctcatttc cggtttccat gtcgaccagg ttggcatccc agaagtagtg aagattcgtt
     2701 gttgtgcctt tataagacac tgagatatcg acccctcctc gatcagcggc gcggccggta
     2761 tgcagcggtt gatgcatgtc gcccaccagg tgggcgacgt aacgaagggc gagtctcctc
     2821 gtcacgatcg gcgcgttctt atcggcgacg gccgctgaaa accagttgag ggcttcaacc
     2881 atacagtttc tgtcagggca gtcgcgtgct tgatcgtagc cgctggcgcg ctcgggaata
     2941 cggacgtagt ggagggggtc gaagtcgcgt atttgtcgcc cctcatggtc cggccaaacg
     3001 gccgtgtccg ccaaggtcat gccggctgga agtatccttt caatctccct gcgcgcgtcg
     3061 gcggtaagat gtgcctgggc gatcaaaccg acgatccgat gtccct
//
LOCUS       DASQKS010000330         5164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3540026, whole genome shotgun sequence.
ACCESSION   DASQKS010000330 DASQKS010000000
VERSION     DASQKS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540026"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VE170_08600"
     CDS             <1..1281
                     /locus_tag="VE170_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15535.1"
                     /translation="TGVFHMRFHFVMAALTTLTLLTRSIEAADYKYPYRDPYLATATT
                     AILSDEGATARVTSTIVHVPGLPGRNNLPTLEGRGDVGLALYRQNRPAPLLFILAGLG
                     SNPYFGIASYLASLFYKEGFHVVTLPSPMSWNFALSASRSGAPGYAPEDARDLYQMMQ
                     KTLTVLRTQHDIKPTAIHFMAVSMGALEGAHLSVIDADEKKIGIAKYLLVNPPLDLAY
                     GVRKIDEWNATAAKLGREKSQQTLAKALAIVDSFAEVGRDNPSVFDRLAKQFADFTTA
                     ELQFLIAENLQSQLPELVYVTQVIHDQKVFTASKNDKRKRLEEAKGFTFTDYDQKIGL
                     PLWQKQIGDPQADLESFIQRGSLAHIVDRLRGNPRVHIMHNADDFMAERKSIEELKEA
                     LGDQVTLYPYGGHLGNLWYPENKEYVLKYFRPRP"
     gene            1545..2165
                     /locus_tag="VE170_08605"
     CDS             1545..2165
                     /locus_tag="VE170_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15536.1"
                     /translation="MNEDPEVLAWNNCHPDDLWIFDKLLLSKKLNYLCGPGCVPVPKA
                     NFYIVRPVMNLYGMGAGAEVKWLTPADSTAVPQGYFWCEEFKGRHLSIDYDRNGVQLT
                     CVEGLRHANAPLYKFSEWKVANQTVQFPEVLKSLVGTYEFYNVEIIGDKIIEVHLRQN
                     HDPIQNDLRVCWEKDTMHENQRLLLNHEDAGGYLPEKRLGFYISKR"
     gene            complement(2467..3393)
                     /locus_tag="VE170_08610"
     CDS             complement(2467..3393)
                     /locus_tag="VE170_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYJ15537.1"
                     /translation="METVARVRGESLGHKQLGAIWKHSGAFAVQTLKGFRANQGLLLA
                     GAVAYYALLSIVPLLILTVIALSYVIDQAELLDTLGRYLEWLTPGQSNLIVVELMNFL
                     DHRGVIGWLLLATMLFFSSLAFTVLENAMSVIFIHRVAIRRRHFLVSALIPYCYILFL
                     GLGMLLVTLVSGSLQAMGQESVDFLGHNWSLGGVSGVLLYLLGFAGEIFVVTSIYLVM
                     PVGRLSLRHALIGGITAAVLWELTRHVLVWYFATLSQVGLVYGSLTTAIAVLLSLDLA
                     ATLLLLGAQVISEYERISSAAQDTPPQPLSTR"
     gene            complement(3381..4346)
                     /locus_tag="VE170_08615"
     CDS             complement(3381..4346)
                     /locus_tag="VE170_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15538.1"
                     /translation="MTKKIAAVVLFTITILSVNAANALDTVTAALTSKAFQYVPLVIA
                     QERGYMREEGLELKLVFMQNAPGLQALIANAVQFSGSGSSALVAISKGNAPLKTILAF
                     NDQVLQWIVARPNIKTLRDLTGKKVATTGVASIAAYMFRNILTKHNIPKDIVLIDPGP
                     VNRLPSLLSGAVDAAIVSPEERYAALDQGMRDLVFIGKEVKNSWGTFATSERFIKEQA
                     KQLAGFVRAVVKGLRVARQERESTIAAISKFSELDKTLSTRMYDDLVNTFTKNGYVDE
                     QSQRNDLAIVAQVAEVNEVVPPKRAYDFSFAIQAEQQLNKQGWKP"
     gene            4732..>5164
                     /locus_tag="VE170_08620"
     CDS             4732..>5164
                     /locus_tag="VE170_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15539.1"
                     /translation="MLKLNIALLLAIAGLAGCSTNAHISRSGTLLSGMTQYHGDMQSL
                     NSVSRWPERQRVAGSLKTVITSTVGASPEFYRLVDLDLRKREFTVTMRDTNLRPDRLR
                     EMQDELVAMDEEIAALKPVVKTQLSAVANHEQSDHIENAATL"
BASE COUNT         1236 a         1359 c         1423 g         1146 t
ORIGIN      
        1 acaggagttt ttcacatgcg ctttcatttc gttatggcgg cattaaccac gttgacgctt
       61 ttgacccggt caatcgaagc cgcggattac aagtatccct accgtgatcc atacttagcg
      121 actgcgacga ccgcgatttt aagtgacgag ggcgccaccg cgcgagttac gtcgactatt
      181 gtccatgtgc ctggcctgcc gggaagaaat aacttgccta ccctcgaagg acgcggcgat
      241 gtgggccttg ctctttatcg ccaaaaccgt ccggcgccgt tgcttttcat tctcgccggc
      301 ctcggatcca acccctattt tggcattgcc tcctatctag cgagtttgtt ctataaagaa
      361 ggcttccatg tggtcacttt gccctcgccg atgagctgga actttgcgtt gtcggccagc
      421 cgtagcggcg cgccgggcta cgctccggaa gacgcccgtg atttgtatca aatgatgcaa
      481 aaaactctga cggtcttgag aacgcagcac gacatcaagc cgacggcgat ccattttatg
      541 gcggtcagta tgggcgcgct tgaaggcgcg catctgagcg ttatcgatgc cgacgagaaa
      601 aagatcggta tcgccaagta tctcctcgta aacccgcccc tcgatctcgc ttacggcgtc
      661 agaaagatcg atgagtggaa cgcgacggcg gctaaattgg gccgggagaa atcgcagcaa
      721 accctagcca aagcgctggc catcgtagat tcctttgccg aggtggggcg cgacaacccg
      781 tcggtgttcg atcgcttggc aaagcaattt gccgacttca cgacggcaga actccaattc
      841 ctcatcgccg agaatttgca aagccagctt cccgagttgg tctatgtgac ccaggtcatt
      901 cacgatcaaa aggtttttac cgcgtcgaaa aacgacaaga gaaaacggct ggaagaagct
      961 aaaggtttca cgtttacgga ttatgaccaa aagatcggtc tgcccctatg gcaaaagcaa
     1021 ataggagatc cccaggccga tctggagagt ttcatacaac gcggttcttt agcacacatc
     1081 gtcgatcggc tgcgcggcaa tccaagagtc cacatcatgc ataacgccga cgatttcatg
     1141 gccgagagga aatccatcga agaactcaaa gaggccttgg gcgatcaggt gacgctctat
     1201 ccctacggcg gccatctcgg caacctctgg tacccggaaa acaaagagta tgtgctgaaa
     1261 tactttcggc cgcgcccttg aacgagagct tcgagttcga gatcatgatg ggcggcacga
     1321 cccagcaatt cgtgccgcgg ttccgttcgc ttcagcggtg ccgaatttgt cgatgagaca
     1381 tcattttgcc gtattgataa ctctcaagac gtgcaagcgc ctcaatttcg atttaccgat
     1441 tgtcggaact gcggtgtcat cgtttatcct aatgccggaa gatttttgtt tcgcacattc
     1501 ctatagcaat ctagcgcgga gcgggtgcta ggcccgaact cgtcatgaat gaagatccag
     1561 aggttcttgc atggaataat tgccatccgg atgatctgtg gatttttgac aagttattgt
     1621 tgagcaaaaa acttaactat ctgtgcggcc caggttgcgt acccgtgccg aaggcaaatt
     1681 tttacattgt ccggccggtg atgaacctct acggaatggg cgccggcgcc gaggttaaat
     1741 ggttgacgcc ggcagattcc accgctgtgc ctcagggtta tttttggtgt gaagagttta
     1801 agggacgcca tttgagtatc gattatgatc ggaacggcgt ccaactcacc tgtgtcgaag
     1861 gcttacgaca cgccaacgcg cctttatata aattctctga atggaaggtc gcgaatcaaa
     1921 ccgttcagtt ccccgaagta ctgaaatccc tggtcggtac ttatgagttt tataatgtcg
     1981 aaatcatcgg cgacaaaatc atcgaagttc atttaaggca aaatcatgat ccgattcaaa
     2041 acgacctcag agtctgctgg gaaaaagata ccatgcatga aaaccaaaga ttgttgctga
     2101 accatgaaga cgcgggcggc tatttacctg aaaaacgcct cggattctat atttcgaagc
     2161 gttgaaatag aaccggcggg tacgaggcga caaatcaatg cctgggcgat gcgcacagtg
     2221 tggatcgaag acctacctgg gacggacaac catacacacc gtcctgcttg aaatccgcaa
     2281 cgttccctgc actgtgtgcc acgaatgtga ccatgagcag atcggccatc aggcccaact
     2341 taaaatcgac aagctcgtgc aacgagcagc gcaagggaaa ctcaaggatc gagtggtgac
     2401 attgtaggcc gttgctgttt tcacggcgtc tggcgtactg gagcttgccg gcgcgaaaat
     2461 taatttttag cgggtgctca atggttgcgg cggcgtatcc tgggcggcgc tgctgatgcg
     2521 ctcatattcg gagatcacct gggcgcctag taatagcaat gtcgcagcga gatccaggct
     2581 caacagcacc gctattgccg tcgttaatga tccgtagact agaccgacct gagacagagt
     2641 agcgaagtac cagacaagca cgtggcgagt gagctcccaa agcactgccg ccgtgatccc
     2701 gccgatcagc gcatgccgca gcgacaaccg tccgaccggc atgacaaggt agatggacgt
     2761 gacaacgaag atttctccag cgaagccgag caaatagagg agcactccag aaacgccgcc
     2821 gagggaccag ttgtgcccca aaaagtcaac gctttcttgc cccatcgctt gcaggctgcc
     2881 cgagacgagg gttaccagca gcatgccgag tcccagaaac aagatgtagc aatacggaat
     2941 cagtgcagag acaagaaaat ggcgtctgcg gatggcaacc cgatgaataa aaattacgga
     3001 catcgcgttt tccaaaaccg tgaatgccag ggagctgaag aatagcatcg tcgccaacag
     3061 cagccagccg atgacaccgc gatggtcaag aaagttcatc aactcaacca cgatcaagtt
     3121 tgattgcccg ggagtcagcc actccaggta gcggccgagg gtgtcgagca attccgcctg
     3181 atcgataacg taggagagcg cgatgaccgt aagaatcagc agcggaacga tcgacagaag
     3241 agcgtagtaa gcgacagcgc ccgctaagag aagtccctga ttggcgcgaa aacccttcag
     3301 cgtctggact gcgaaagcgc ccgaatgctt ccatatggcg cctaactgct tgtgacccaa
     3361 tgactctcct cgaacccttg ctacggtttc catccctgct tgttcagctg ctgttcggct
     3421 tgaatggcaa agctgaaatc gtaggcgcgc tttggcggga ccacttcatt cacttccgcg
     3481 acttgggcga cgatggcgag gtcgtttctt tgactctgtt catcgacgta gccgttcttg
     3541 gtaaaggtgt tgaccaggtc gtcgtacatg cgcgtcgaca gcgtcttgtc gagttcgctg
     3601 aatttagaaa tggcggcgat ggtgctttca cgttcctggc gggcgacgcg caggcctttg
     3661 accacggcgc ggacgaagcc ggcgagctgt ttagcctgtt ctttgatgaa tcgttcggaa
     3721 gtcgcaaagg tcccccagga gtttttcact tccttgccga tgaagacaag gtcgcgcatg
     3781 ccttggtcca gcgcggcgta gcgctcctca gggctgacga tggcggcgtc gacggcgccg
     3841 gacaagagtg acggcagccg attgaccggg cctggatcga tcagcacgat gtctttgggg
     3901 atgttgtgtt tggtcaagat atttctaaac atgtatgcgg cgatcgacgc gacgccggtg
     3961 gtggcgacct ttttgccggt caaatcgcgc agggttttaa tgttgggccg ggcgacgatc
     4021 cactgcaaca cttggtcgtt gaatgccagg atagttttca gcggcgcgtt gcctttggag
     4081 atcgccacca gtgcgctgct gccggagccg gagaactgaa cggcattggc gatcaaggct
     4141 tgcaggccgg gggcgttctg catgaagacc agctttagct caagtccttc ctctctcatg
     4201 tagccgcgct cctgggcgat aaccagcggg acatattgaa acgctttcga ggtcaaagcc
     4261 gcggtaacgg tatcgagggc atttgctgcg ttaaccgata ggatcgttat ggtgaaaagc
     4321 acaactgcag cgattttctt cgtcatgaaa gtctgtcctc cagcgcctga cggggcgttt
     4381 tgtattaaac tcagtagcct ttatgtctta agccatgctg cttctcaata gcgccagccg
     4441 ccggtgctgc cccagcaatt gacctaagaa aagatgctcg tccgctggga agcgtgaata
     4501 gcggggtgct ggagaattat tacagtagat ccggtattca actgtgaccg tgaagcggta
     4561 aaaaaacgcc gctctgtccg ggccaaaact cgtggcggaa ttgatgcagg tataacagca
     4621 agtcaatttg cccaaacgga cacggcttcg acagctaaat ggattcgaca cggtccggcc
     4681 agggttaaaa taaccttcag cggtttgggc cgatgtggta ttcttagcga catgctgaaa
     4741 ttaaacatcg ccttgttgtt ggccatagcc gggctggctg gatgttcaac gaacgcgcat
     4801 attagccgta gcggcacgtt actctccgga atgacccagt atcatggaga tatgcagtct
     4861 cttaatagcg tttcacgctg gccggaacgg cagcgcgtcg ccggctcgtt gaagacggtt
     4921 atcaccagca cggtcggcgc gtcgcctgaa ttttaccgcc tggtcgatct cgacctgcgc
     4981 aagcgcgaat tcaccgtcac catgcgcgat accaatctcc gtcccgaccg gttgcgcgag
     5041 atgcaggacg agttggttgc catggacgaa gagatcgccg cgctcaaacc ggtggtgaag
     5101 acccaacttt cggcagtcgc gaatcacgag caaagcgacc acatcgaaaa cgccgcgact
     5161 ctag
//
LOCUS       DASQKS010000331         9955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5207820, whole genome shotgun sequence.
ACCESSION   DASQKS010000331 DASQKS010000000
VERSION     DASQKS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5207820"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1370
                     /locus_tag="VE170_08625"
     CDS             321..1370
                     /locus_tag="VE170_08625"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15540.1"
                     /translation="MNKCRDWQAGMRRILIRPLALFSLLLTIVSTSFAQVNPYLARPS
                     ERPVSVRIATCAVSGGFAHLYTALENDLFEKYGFKMEHVFIRGSAPALAALMADEIQF
                     LYCAADATLPALAAGIDVKLVAAPLVKLPYVLVTRKEIRRIEDLKGKALGVARAGDLS
                     DRLSRAMVKRFNIADVTMRPIGGSQSERYQALAANLVQGVVITPPLDVRAKNDGYNVL
                     YRLIDMDIPFIYSSLHATSRTVRDRPEMVQRMVAAFAEAIHYVDKNPVKTKSAIAKIM
                     RTKDDEALQVSYNVYTKDIVDRRMIVPGQAVNDSLDLYRSLGAPIKRKAEDIYDNSFV
                     NHLEKSGFYRGLWGR"
     gene            1611..3053
                     /locus_tag="VE170_08630"
     CDS             1611..3053
                     /locus_tag="VE170_08630"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HYJ15541.1"
                     /translation="MKTLIQGGWVVGFDGGEHEIIKDGVVVFDGNRVEFVGKEYLQPV
                     DKKLDAKGCLVSPGFIDTHFHSGINASDYILADSTKTDFFASNYLAHGGPTREGAHSA
                     HLKDVDIGQRFSLIHVLKSGVTTTFDIGGAGGEPARYVDMIDQVGIRCVAGPSYKNVA
                     FFHDKVGRLEYDWNDERGSKGLQTAVDFAKKFNGACSGRMTAMLFPGHVDSCTPALLK
                     ETKTAAKDAGVGIQIHATINLFEFHTVMQRERCTPIELLKKLGFLAPDVSLSHCIFVS
                     GHSWLAYPYGDDLKIIADSGASVAYSPLKYLKLGILMESFDRYRQAGINMGIGTDTFP
                     KDILSDMRYAGIASRIADKSFLAGHPRDVFNSITLGGAKMIGRDDIGRLAKGAKADIA
                     IVNLRDIAFGAVHDPIRSLMETAVSRDVRTVIVDGETLVDNGKFLRLDEQELLDKVQT
                     KGEQVWDSVAKWHWTGKPIDEVVPPSFRMK"
     gene            complement(3119..4906)
                     /locus_tag="VE170_08635"
     CDS             complement(3119..4906)
                     /locus_tag="VE170_08635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HYJ15542.1"
                     /translation="MANNSAAGIRWDLSDLFAAHDDEKIETTLNDCRARAMAFAERYR
                     AALNSPPTLSAETVRDALQELAVIYEALGRVGSYAGLLYAADTAKPEYQDLEQQVEQR
                     STEIRNLLLFFELAWLKVEDDFAAKLMRDDQLKPYRHYLTSLRRYRPHTLSELEEKLF
                     NEKDNTGRNAFGRLFSEITSALIFRLERDGKTEELNLSQILSLLHEPDRSLRQRAMET
                     VYQELSQHGQVLTFIYDTLIQDNLTTDRLRHYPDPMAPRHLSNEIDGAAVKTMMEVTE
                     KNYPLAHDYFRLKAKLLELPQLALYDQYAPVGKEVRPFPYAKAKEVILNAFEAFDPSF
                     RNLAGEFFAKNWIDAEIRKGKRGGAFCASPSPQLHPYILCNYDDNLRDVMTVAHELGH
                     GLHGCLSRKQNYFNYDTPLTTAETASVFGEMLVFDYLLDQQSDREVQIALLAGKIEDI
                     FATVFRQNVLTRFEELAFSARQEKRLTPEAIGQLWLEANGKYYGDAVAMPDSYRWGWS
                     YIPHFIHSRFYCYSYVFGQLLVLALYRMYKDQGKSFVPKYVALLEAGGSDTPDALLKP
                     LGVDIHQAEFWQKGFEEIRGLTTKLNALI"
     gene            5038..5748
                     /locus_tag="VE170_08640"
     CDS             5038..5748
                     /locus_tag="VE170_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ15543.1"
                     /translation="MAELTELKKKLAMSSRMLFNSGLVDYSGHISVRIPGSDHLLILP
                     HPVSRAIVKPDDMVVTDFDGKVIEGNYKAPSEVFMHARAYKARSDIQSVAHLHNHMVA
                     TLSMVDKPFYPASSNPGAFFGPGALPKYMDPALIHTIEQGDAVAKALGKADALMLRGH
                     GSMVVAQSIEWVFAACVDLEEAAARFYKAALLGPVLVYNEDETQRVMKGRRKDSVVQK
                     VWDHNVAKTKLAGLMEGI"
     gene            5974..6354
                     /locus_tag="VE170_08645"
     CDS             5974..6354
                     /locus_tag="VE170_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15544.1"
                     /translation="MYDDFLATILEPDTFLPSQFYGNGGLARALEGEKRLMIAIMKDA
                     VECLEKYRGSRNGAGKISYQSAIEWVEDTDTDWLYSFTNICDLLGFDPHYLREQLLKR
                     ENRYVKTLRTRNLSLASMGASAAH"
     gene            complement(6357..7775)
                     /locus_tag="VE170_08650"
     CDS             complement(6357..7775)
                     /locus_tag="VE170_08650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYJ15545.1"
                     /translation="MTLLPRRIRGWPACGVSLFLIVMLGSSCRSVAPLLPSFTPPAED
                     EEVRISREFRREAKKHLKFITHPEVERYVDRIARRILSATGPLSFDYRFFVVEDPQLN
                     AFAVPGGSIYLFTGLIERAKSTDELAGVLGHEIVHIKGRHMARSSGLDAISVLSLLAT
                     VLLARSGSGGQAAGVVGQAVAATRQAAYSRQLEMESDTLGTRYIAAAGFDPRGTLAFL
                     KTMDQERALNPIDVPAYILSHPLTQERVANAELVVKSFAPNSPRHEDNEALKKVQILI
                     RMERTNREAVVAEYEKLVQKDPDNAELLHLLGFAQFNMGLLAPAQKNYEKSWRLRPDH
                     LGLLREIGRLSSQTGDFASARKAFDRAIELEPNEPLHYYYLGEFLEKTGDLRSAAGAY
                     LNAQSLAPLWDKPVFRLGQVYGKLNRLADGYYYLGRAQLLQDEDERAIADFERALKLV
                     GASSPRGQAINEELKALRSRKR"
     gene            7969..>9955
                     /locus_tag="VE170_08655"
     CDS             7969..>9955
                     /locus_tag="VE170_08655"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ15546.1"
                     /translation="MTNRFEIPSDKLAWRCDLSYLPFTCTADMTPLEDFIGQDRAIRA
                     IEFGLGVNKPGFNIFVTGLTGTGKTSIIKAFLKKVNGEKAAPADAPTPEDWCYVYNFD
                     DPDRPLALKLRRGAGKALKSDMEQLIQTLQRDAKKLFESDEFAHQRQGLIEQLQKKQQ
                     EMMEGLMAQAGQAGFALRMTPSGIVLLPTKDGKPMAEEDYLGLAPTEKEQLEERRSVI
                     EKQVEDALRDGKKLEREIAAKLETVEAEAADYLVRNPLTELKDKYHDYPRILAYLDGV
                     REHILKNLQRFKVETTAPASPPGATTYGERPGDPFLPYRVNVFVDNGDTQGAPIIVET
                     NPTYHNLFGVIEKKPIMGGYTTDFTMIKAGSISKANGGYLVLYDREVLANSGCWEALQ
                     RVIKNRELRIEEPGTFFGFVPPQGLRPEPIPTDTKVIMIGDPSLYRMLATGDPDFRET
                     FKVKADFNFEVDRTQENITAFACFISDYCKREKIRHFDSEGIARVIEQCARQVEDQNK
                     LSTRFSDMVDLLIESDYWAGKENAALVSGKHVERALEEKTFRLNLIEKRLQELIAEGT
                     VLVDVTGAAVGQVNGLAVYQMGDFSFGKPSRITAKTFMGRGGIVNIERESKMSGRSHD
                     KGVLILGGYLGGKYAQHSPLSLSSTICFEQSYDGVDGD"
BASE COUNT         2199 a         2699 c         2879 g         2178 t
ORIGIN      
        1 tgaaacgctg accagcgatg gtcgaaaccg ctcagcttgc catgtctccc ccgtagcgca
       61 ggcaactgag tggttttgtt ttttcttcca taatttttcg acagttaaga aggcgagtcc
      121 acttgatctc ggctctggcg cggccattgc ccgcccatgg cgccgtcgcg tccacgccct
      181 gctcccgtgt cctataaata tcgcgcgatg aatatggggt ttcgctggat tgacagccat
      241 cgagaatcgt ctaatatccg ccactgcggg tggccggaat aagatctaag cgaggtatcg
      301 tcaatggatc cgggtgggca atgaataaat gtcgcgattg gcaagccggc atgcgcagga
      361 ttttaattcg gccattggcg ctgttctcac tgcttcttac cattgtcagc acttcgttcg
      421 cccaggttaa tccctatctc gcccgtccga gcgaacggcc cgtgagcgtc cgcatcgcga
      481 cttgtgccgt gtccggtggc ttcgcccatc tctacaccgc actggaaaat gatcttttcg
      541 aaaaatacgg attcaaaatg gagcatgtgt ttatccgtgg cagcgcgccg gcactggccg
      601 cgctgatggc cgacgagatc caatttcttt actgcgccgc cgatgccacg ctgccggcgc
      661 tggcggccgg cattgacgtt aagctggttg ccgcgccgct ggtcaaactg ccctacgttt
      721 tggtcacccg caaggagatc cgccgcattg aggatctcaa aggcaaggcg ctaggcgtgg
      781 cgcgagccgg cgatttgagc gaccggttgt cgcgtgccat ggtcaagcgt tttaacattg
      841 cggatgtcac catgcgtccc atcggcggca gtcaatcgga acgctaccaa gcgctggcgg
      901 cgaacttggt tcaaggcgtg gtgatcacgc cgcccctcga tgtgcgcgcc aaaaacgatg
      961 gttacaacgt gctgtaccgc ctgatcgaca tggatattcc gtttatctac agttccctac
     1021 atgcgacctc gcgcaccgtg cgcgaccggc ccgaaatggt ccagcgcatg gtcgcggcat
     1081 ttgccgaagc gattcattac gtcgataaga atccggtgaa aactaaatcc gcgattgcta
     1141 aaatcatgcg tacgaaagac gacgaagcct tacaggtatc ttacaatgtc tataccaaag
     1201 acattgtcga tcggcgcatg atcgtcccgg gacaagcggt gaacgactcg cttgatctct
     1261 atcgttcgtt gggtgcaccg atcaaacgta aggccgaaga tatctacgac aacagcttcg
     1321 tcaatcactt ggagaaaagc ggtttttatc gagggctctg gggccggtaa gactggaaaa
     1381 aagcggtctc tcaaagactg tgcggcagcc gattggggcg cccggcgcgt tgacccgtcc
     1441 agtggttggc gattacgagc aatcgctcag acttcgttcg aatccatcaa gaagatatct
     1501 ctctctaaat cgaaatagca aacaggtttt ccgtttgcgg cttgtcttaa gaatcttctt
     1561 ttgttagtcc tgccactgac tcgcattcca atcccggaaa ggcacgggca atgaaaacac
     1621 ttatccaagg cggctgggtc gtcggcttcg acggcgggga gcatgaaatc atcaaagacg
     1681 gcgtggtggt tttcgacggc aaccgcgtcg agttcgtcgg caaagagtac ttgcaaccgg
     1741 tcgataaaaa gctcgacgcc aaaggctgct tggtctcgcc tggtttcatc gatacccact
     1801 ttcactccgg tattaacgcc agtgactaca ttcttgccga ctcgactaag acggacttct
     1861 tcgcgtcgaa ttatctcgcc catggcgggc caacccgcga aggcgcgcac tcggcgcatt
     1921 taaaggatgt cgacattggt cagcgttttt cattaattca cgttttgaaa agcggcgtga
     1981 ccacgacctt cgacatcggc ggcgccggcg gcgagccggc acgctatgtc gacatgattg
     2041 accaggtcgg cattcgttgc gtcgcaggtc cgagctacaa gaacgtcgcc ttttttcacg
     2101 ataaagtggg ccggttggaa tacgattgga acgacgaacg cggcagcaaa ggcctccaga
     2161 ccgcggtgga cttcgccaaa aaattcaacg gcgcgtgcag cggccgcatg acggcgatgt
     2221 tatttcccgg ccatgtcgac tcgtgtacgc cggcgttgct gaaggaaacc aaaaccgccg
     2281 ccaaggatgc cggcgtcggc attcagattc atgcgacgat caatttgttc gagtttcata
     2341 cggtcatgca acgcgaacgc tgcaccccca tcgagctatt aaaaaagctc ggcttcctcg
     2401 cgcctgatgt ttcattgtcg cactgcatct ttgtcagcgg ccatagctgg ctcgcctatc
     2461 cctacggcga cgatcttaaa atcatcgccg actcgggcgc gtcggtagcc tatagcccgc
     2521 ttaaatattt gaagctgggc attctcatgg aatccttcga ccgttaccgc caggccggta
     2581 ttaacatggg catcggcacg gacactttcc ccaaagacat tctatccgac atgcgctacg
     2641 ccgggatcgc ctcgcggatc gccgacaaaa gttttctcgc cggtcatccg cgcgatgttt
     2701 tcaattcgat cacgctgggc ggcgccaaga tgatcggccg cgacgacatc ggccggctag
     2761 cgaaaggcgc caaagccgac atcgccattg tcaatctgcg cgacattgcc ttcggcgccg
     2821 tgcacgatcc gatccgctcg ctgatggaaa ctgccgtcag tcgcgatgtc cgcaccgtaa
     2881 tcgtcgacgg cgaaactctg gtcgacaacg gcaagtttct gcgcctagac gaacaggagc
     2941 tgctcgacaa agttcagacc aagggcgaac aggtctggga cagcgtcgcc aaatggcact
     3001 ggaccggcaa accgatcgac gaagtggtgc cgccgagttt tcggatgaag tagcaatctg
     3061 cgcagccggc taagcgaaag atcttaggag gcttggttgg gggcacggca cggcgatact
     3121 agattaacgc atttaatttc gttgtcagtc cacgaatctc ctcgaaaccc ttctgccaaa
     3181 actcggcctg gtgaatatcg acgccgagcg gtttgagcaa tgcatccggc gtgtccgagc
     3241 cgccggcttc gaggagcgcg acgtacttcg gcacgaagct tttgccttgg tccttgtaca
     3301 ttcgatacag ggcgaggacc aacagctggc cgaagacgta gctgtagcaa tagaagcgcg
     3361 aatggatgaa gtggggaatg tagctccagc cccagcggta gctgtcaggc atggcgacgg
     3421 cgtcgccgta gtatttgccg ttggcttcga gccagagttg gccgatggct tcgggtgtca
     3481 aacgtttttc ttgtcgcgcc gagaaagcca gctcttcgaa tcgggtcagg acattttgcc
     3541 ggaagacggt ggcgaagatg tcttcaatct tgccggccaa gagcgcgatc tgcacttctc
     3601 gatcgctttg ctgatcgagc agataatcaa acactagcat ttcgccgaac accgaggcgg
     3661 tctcggcggt ggtgagcggg gtgtcgtagt tgaaataatt ttgcttgcgg ctgagacagc
     3721 cgtgcaggcc gtggcctaat tcatgagcga cggtcatgac gtcgcgcagg ttgtcgtcgt
     3781 agttacacag aatataggga tggagctgtg gagaaggcga agcgcagaag gcgccgccgc
     3841 gtttgccttt gcggatctcg gcgtcgatcc aatttttggc gaaaaactct ccggcgaggt
     3901 tacgaaaact cgggtcgaag gcttcaaagg cattgagtat cacttctttc gctttggcat
     3961 aggggaacgg cctgacttct ttgccgacgg gggcgtattg gtcgtagagc gcgagctgcg
     4021 gcaattcgag cagcttcgct ttcaagcgaa agtaatcgtg ggcgagagga taattctttt
     4081 ccgttacctc catcatggtt tttaccgctg cgccgtcgat ctcgttggac aagtgacgcg
     4141 gcgccatggg atccggatag tgacgcaaac gatccgtcgt caggttgtct tggatcaagg
     4201 tatcgtagat gaaggtcagc acctggccat gttgtgacag ctcttgatag accgtctcca
     4261 tggcgcgctg gcgcaggctg cgatccggct cgtggagcag cgataggatc tggctcaaat
     4321 tcaactcttc ggtcttgccg tctctttcca gtctgaaaat cagcgccgag gtgatttccg
     4381 aaaacagccg gccgaaagca ttgcggccgg tgttgtcttt ttcgttgaaa agtttttctt
     4441 ccaattcgga cagcgtgtgc ggacggtaac ggcgcaggct ggtgagataa tgccggtaag
     4501 gcttaagctg gtcgtccctc attaatttgg cggcgaagtc gtcttcgacc ttgagccagg
     4561 cgagctcgaa aaacagcagc agatttctaa tttcagtgct gcgctgttcg acctgctgtt
     4621 ccaggtcttg atactccggc ttggcggtat ccgcggcgta aagcaggccg gcgtagctgc
     4681 cgacccggcc gagggcctca tagatcacgg ctaattcctg caaggcgtcg cgcacggttt
     4741 cggcgctcag tgttggcggg ctgttcagtg ctgcccggta acgctcggcg aacgccatcg
     4801 cccgagcgcg gcagtcgttt aaggtcgttt cgatcttctc gtcgtcatgg gcggcaaaaa
     4861 ggtcgcttaa gtcccagcgg attcccgccg cgctattatt ggccatggta aggtcctttc
     4921 gctgagctgt tacaactatt tcacggggaa accggcaaga gtcaagttgc gacttcactg
     4981 tttcagtgta tatgatgacc ggaatttttc cgtaactagt ttgaaagggg tttatccatg
     5041 gctgaattaa ctgaactcaa aaagaagctg gcgatgtcgt cgcgcatgct gtttaactcc
     5101 ggactggtcg attacagcgg ccacattagc gtacgcatcc ccggctccga tcatttgctg
     5161 atcctgccgc acccggtgag ccgcgcgatc gtcaaacctg acgacatggt ggttaccgac
     5221 ttcgacggca aggtgatcga gggcaactac aaagcgccgt cggaagtttt tatgcacgcg
     5281 cgcgcttaca aagcgcgcag cgatatccag agcgtggcgc atctgcacaa ccacatggtg
     5341 gcgaccctga gcatggtcga taagccattc tatccggctt cgtccaatcc cggcgccttc
     5401 ttcggtcctg gcgcgttgcc caagtacatg gacccagcgc tgattcatac catcgaacag
     5461 ggcgatgccg tggccaaggc gctcggcaag gcggatgcct tgatgctgcg cggtcatggg
     5521 tcaatggtcg tggcgcaatc gatcgagtgg gtgtttgccg cctgcgtcga tcttgaggaa
     5581 gcggcggccc gcttctacaa ggccgcgctg ttagggccgg tgctagttta caacgaagat
     5641 gaaacccagc gggtcatgaa aggccggcgc aaggactcag tggtgcaaaa agtctgggat
     5701 cacaacgtag cgaagaccaa attagccggg ctcatggagg gtatttaatt tcgctccaaa
     5761 gattttgtcg tgacttttcc ttcatcaagc cgcctcggaa cgaatgcaat ggacatcatc
     5821 cgagggaatt ttcgacgacc atatttaatt ctaagccgcc gtctcatacg gcggtttttt
     5881 agcgcgtaac ccggcaatgg attttttttc ttgcctgcaa ctataaccct gtgttatgaa
     5941 tccggtttaa ataattgagc ggagcattga gaaatgtacg atgattttct tgcgacgatc
     6001 ctagaaccgg atacctttct gccatcgcaa ttctatggca atggcggcct ggcgcgcgcg
     6061 ctagaaggtg aaaaacgctt gatgatcgcg atcatgaaag acgcggtgga gtgcttggaa
     6121 aaataccgcg gctcgcgcaa tggcgccggc aagatcagct atcaaagcgc cattgagtgg
     6181 gtggaagata ccgatactga ctggctctat tcatttacca atatctgcga tttgttggga
     6241 tttgatcccc actatctacg tgaacagctg ctcaagcggg aaaaccgtta tgtcaagacg
     6301 ctgcggacga gaaatttgtc tttagcgtcg atgggtgcga gcgcagccca ttaaactcag
     6361 cgtttacgac ttctaagggc cttcagctct tcattgattg cttggccgcg cggcgaactt
     6421 gcaccgacca gtttaagcgc tcgctcaaag tcggcgatgg cgcgctcgtc ttcatcttgc
     6481 agcagttgcg cccggcccag atagtaataa ccatctgcga gccggttgag cttgccatag
     6541 acctggccca agcgaaatac cggtttgtcc cacaatggcg cgaggctttg ggcattgaga
     6601 taggctccgg cggcacttcg caagtcgccg gtcttctcga gaaactctcc gagataatag
     6661 taatgtagcg gttcgttggg ttcgagctcg atggcccggt cgaaggcctt gcgagccgag
     6721 gcaaaatcgc cggtttgact cgacagccgg ccgatctcgc gcagcaggcc gagatgatcg
     6781 ggccgtaacc gccatgattt ttcgtagttt ttctgagctg gcgcgagcaa gcccatgtta
     6841 aactgagcga agccaagcag atgcagcagt tcggcgttat cggggtcctt ttgtaccagc
     6901 ttctcgtact cagcgacgac cgcttcgcga ttggtgcgct ccatgcggat caggatctga
     6961 actttcttta aggcctcgtt gtcttcgtgc cgcggactat tgggagcgaa ggatttgacg
     7021 actagctcgg cattggcgac ccgttcctga gtcagtggat ggctcaaaat gtaagccggg
     7081 acatcgatcg ggttgagggc gcgctcctgg tccatggttt tgagaaacgc cagcgtaccg
     7141 cgcggatcga agccggcagc ggcaatatag cgggtcccca acgtatcgga ttccatctct
     7201 agctgacggc tgtaggcggc ttgccgggtg gcggcgacgg cttgaccgac gacaccggca
     7261 gcctgtccgc ccgagccgct gcgcgccaac aagacggtgg cgagcaagct caataccgat
     7321 atagcgtcga gtcccgatga gcgtgccata tgacgccctt tgatgtggac gatttcatgg
     7381 ccgagcactc cggctaactc gtcggtggac ttcgcccgtt cgataagacc ggtgaagaga
     7441 tagatcgatc cgccggggac ggcaaaggcg ttcagctgcg ggtcctcgac gacaaaaaat
     7501 cggtagtcaa aagacagcgg accggtggcg ctgagaattc gccgggctat gcgatcgaca
     7561 taacgttcga cttctggatg ggtgataaac ttcaaatgct ttttcgcctc gcggcgaaac
     7621 tcgcggctga ttctgacttc ttcgtcttcg gcgggcggag taaaggacgg caatagcggc
     7681 gccacggatc ggcagctcga gccgagcatg acgatcaaaa acagcgatac gccgcatgct
     7741 ggccatcccc gaatgcggcg cggcagtagt gtcattgacg aagtatagag agcgggctgc
     7801 ggcggttcaa ggttaaagcc ggattgccag ttccagttaa tcgcggcgcc aggggcaggc
     7861 cgtggccgcc gccgctcgat atcgatgcca tcgttgacgc gacgttggtt gacgaccccg
     7921 gggcaagtcc ctattgtcgg tcaacgataa acaactagag gaaccgcgat gaccaaccgt
     7981 ttcgaaatac cgtctgacaa actcgcttgg cgctgcgatt tatcgtattt gccgtttact
     8041 tgcacggcgg atatgacgcc gctggaagac tttatcggcc aagatcgggc gattcgagcg
     8101 atcgagttcg gcctcggcgt caacaaaccg ggcttcaata tttttgtcac cggtctgacc
     8161 ggcacgggta aaaccagcat catcaaggcg tttctaaaga aagttaacgg tgagaaagcg
     8221 gcgccggccg atgcgccgac acccgaagat tggtgctatg tttacaactt tgacgatccc
     8281 gaccggcccc tggcgttaaa gctccgccgt ggtgccggca aagccctcaa gagcgacatg
     8341 gagcaattga tccaaactct ccagcgcgac gcgaagaaac tcttcgagag cgacgagttc
     8401 gcgcaccagc gccaggggct gattgaacag ctgcaaaaga aacagcagga gatgatggaa
     8461 ggtctgatgg cgcaagccgg acaagccgga ttcgccctgc gcatgacgcc atccgggatc
     8521 gtgctactgc cgacgaaaga cggcaagccg atggcggagg aggactatct cgggttggcg
     8581 ccaaccgaga aagaacagct ggaagagcgg cgcagtgtaa tcgaaaagca agtcgaagac
     8641 gctctgcgcg acggcaaaaa gctcgagcgc gagatcgcgg caaaacttga gaccgtggag
     8701 gccgaagcgg ccgactattt ggtgcgcaat ccattgaccg agctcaaaga caaatatcac
     8761 gactacccta gaatactggc ctaccttgac ggcgtgcgcg aacatatttt aaaaaacttg
     8821 cagcgcttca aagttgagac gacggcgccg gccagccctc cgggcgcgac gacctacggc
     8881 gaacggccgg gcgatccgtt tctaccgtac cgggtcaatg ttttcgtcga caacggcgat
     8941 acccaggggg cgccgatcat cgtcgaaacc aacccgactt atcacaatct tttcggggtc
     9001 atcgagaaaa aacccatcat gggcggctac accaccgact ttacgatgat caaagccggt
     9061 tctatcagta aagctaacgg cggctatctg gtgctttacg atcgcgaggt gctggccaat
     9121 tccggttgtt gggaagctct tcaacgagtc ataaagaatc gcgagttgcg catcgaggag
     9181 ccgggcactt ttttcggctt cgtgccgccc cagggactac ggccggaacc gatacccacc
     9241 gataccaaag tgatcatgat cggcgatcct tctctgtacc gcatgctggc gaccggcgac
     9301 cccgacttcc gtgaaacctt caaggtaaaa gcggacttca actttgaagt cgaccgcacc
     9361 caggaaaata tcacggcgtt cgcctgcttc atcagcgact attgcaaacg ggaaaagatc
     9421 cgccacttcg atagtgaagg aattgccagg gtgatcgagc agtgcgcgcg ccaggtcgag
     9481 gatcagaaca aactgtcgac ccgtttcagc gatatggtcg atctcttgat cgagtccgat
     9541 tactgggccg gcaaggagaa tgcggcactg gtatccggca agcatgtcga gcgtgcgctg
     9601 gaggaaaaaa cctttcgcct caacttgatc gagaagcggc tacaggagct gatcgccgaa
     9661 ggcacggtgc tggtggatgt taccggcgcg gcggtcggtc aggtcaacgg cctggcggtt
     9721 taccagatgg gcgattttag tttcggcaag ccgtcacgta tcaccgcaaa aactttcatg
     9781 ggccgcggcg gtattgtcaa catcgagcgc gagtcgaaga tgagcggcag gagtcacgac
     9841 aagggagtgc tgattctcgg cggctatttg ggcggtaagt acgcgcagca cagtccgctg
     9901 tcgctctcga gcacgatttg tttcgaacag tcctacgacg gcgtcgacgg cgaca
//
LOCUS       DASQKS010000332         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1845465, whole genome shotgun sequence.
ACCESSION   DASQKS010000332 DASQKS010000000
VERSION     DASQKS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845465"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2721
                     /locus_tag="VE170_08660"
     CDS             <1..2721
                     /locus_tag="VE170_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HYJ15547.1"
                     /translation="RFVSGYLIQLTADVKSLDGPSGPEKDFTDLHAWAEVYLPGAGWI
                     GLDPTSGLFAGEGHLPLACTPEPGSASPVTGVIDECKVEFSHEMSVTRIWEAPRVTKP
                     YTEEQWQAIDRLGHAVDVDLKANDVRLTMGGEPTFVSMDDPEAEEWSTAALGPNKRRL
                     ATEVYQRLRAHYGAQGLVHFGQGKWYPGEQLPRWSLNCYWRADGEPIWRNDWLIADET
                     QTGNATTELASKFLREVAQRLGLDEQNIFPAYEDLFYYLWRERRLPSNVDPFDSRLED
                     PLERERLMAIFGQGLKQVVGFALPVARDPRTEHWRSGQWFLRGERCYLVPGDSAMGYR
                     LPLDSLPWTSANDYPYVHPPDPTRPLPPLATHGQVRFPVDPGKPVESTASRLDRERAP
                     GKHESAAWISRTAICAEARNGVLYIFMPPTAAVEDYLELVAAVEATATALNQPIMFEG
                     YEPPRDPRLPHFRITPDPGVIEVNIHPSSSWAESSEQTVFLYEAARQSRLSTEKFMID
                     GRHTGTGGGNHFALGGATPADSPWLRRPDLLRSFLSYWHNHPSLSYLFSGLFIGPTSQ
                     APRIDEARNDSLRELEVAFRELERRLESHRDDCPPWLIDRLLRHLLIDVTGNTHRAEF
                     CIDKLYSPDSATGRLGLLEMRAFEMPPHARMSLTQQLLIRALVARFWKVPYAPPRLAR
                     WGTQLHDRFLLPHYIWLDFADVISELHQFGYAFDAGWFAPHFEFRFPKYGDFAVRGIE
                     LELRAALEPWHVLGEEGAPGGTARYVDSSLERVQVKVTGLPPDRYAITCNQAPLPLQA
                     TGTAGEYVAGVRYRAWQPPSALHPTIAVHTPLTFDIVDRWMKRSLGGCQYHVMHPGGR
                     NYEVFPVNAFGAESRRLERFFRIGHSPGIMEIGDAGIDPEFPFTLDLRKI"
     gene            complement(2803..3060)
                     /locus_tag="VE170_08665"
     CDS             complement(2803..3060)
                     /locus_tag="VE170_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15548.1"
                     /translation="METLANKWAERVHTEDGFDADAFFEMCTTSGLVIETIDIPGCYD
                     AYKKLKDGSYIRCEITEHGGDRTYDNDEEFNKAFTVEKKKE"
     gene            complement(3154..3450)
                     /locus_tag="VE170_08670"
     CDS             complement(3154..3450)
                     /locus_tag="VE170_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15549.1"
                     /translation="METFWKKRWSVKGKSFKDTYPNEAHAIETELAKLFAEARGSRDI
                     WCEKLLDRYPTRKEWEENPERSEFIHNLFELAGFRVRYTLGDLPGWIVKNDWAV"
BASE COUNT          754 a         1124 c          998 g          848 t
ORIGIN      
        1 cgctttgtct cgggctactt gattcagctc accgccgatg tgaagtctct cgacggccct
       61 tccggtccgg aaaaggattt taccgatctg cacgcctggg ccgaagtata tctgccgggc
      121 gcggggtgga tcgggcttga cccgacctct gggttgttcg ccggagaagg acatctgccg
      181 ctcgcctgca cgcccgagcc gggatcggct tcgccggtga ccggcgtgat cgatgaatgc
      241 aaagtagaat tttcccatga aatgtccgtc accagaatat gggaagcgcc gcgcgtcacc
      301 aagccctaca ccgaggaaca gtggcaggcg atcgaccggc tcggtcatgc tgtcgatgtc
      361 gacctgaagg caaacgatgt cagactcacc atgggcggcg aaccgacttt cgtctcgatg
      421 gacgatccag aggccgaaga gtggagtact gcggcccttg gcccgaacaa acgacggctc
      481 gcaaccgagg tctatcagcg cctgcgggct cactacggcg cccaaggcct ggttcatttt
      541 ggccagggca agtggtatcc cggcgagcaa ttgccgcgct ggtcgctcaa ttgctattgg
      601 cgcgccgacg gcgaacccat ctggcgcaat gattggttaa tcgccgacga gacccaaacc
      661 gggaatgcaa ccaccgaact cgcgagtaag tttcttcggg aggtagcgca gcgactgggc
      721 ttggacgagc aaaatatttt cccggcatac gaagacctgt tttattatct ctggcgcgag
      781 cgccgtttgc ctagcaacgt cgatccattc gattcacggc ttgaagatcc gctcgaacgt
      841 gagcgactca tggccatttt cggtcaaggc ctaaaacagg ttgtcggttt tgccctgcct
      901 gtggcgcgcg atccgcgcac ggaacattgg cgcagcggac agtggttttt gcgcggcgag
      961 cgttgctatc tcgtccccgg tgattcagct atgggttatc ggctaccgtt agactcgttg
     1021 ccctggacca gcgccaatga ctacccctat gttcatccgc cggatcccac ccggccgctg
     1081 ccgccgcttg ccactcacgg gcaagttcgc tttccagttg acccaggtaa gcccgtggaa
     1141 agcaccgcat cgaggctcga ccgcgagcgc gctcccggca aacacgaaag cgcggcatgg
     1201 atctcgcgca ccgcaatctg cgccgaagcg agaaacggcg tgctttacat cttcatgccg
     1261 cccaccgccg ccgtggaaga ttatctcgaa ctcgtcgccg ccgtggaagc gaccgccacc
     1321 gcgctcaatc agccgatcat gttcgaaggc tacgagccgc cgcgcgatcc gcgcctgccg
     1381 cactttcgca tcactccgga cccgggcgtg atcgaggtca atattcatcc gtcgtcgagt
     1441 tgggccgagt cgagcgaaca gacggttttt ctctacgaag cggcgcgcca gtcgcgtctc
     1501 tcgacggaaa aattcatgat cgacgggcgc cacaccggca ccggaggcgg caatcatttc
     1561 gccctgggcg gcgccacgcc ggcggattcg ccgtggctca ggcggccgga tctgttgcgc
     1621 agttttttaa gctactggca taatcatccg tcgttatctt atcttttcag cgggcttttc
     1681 atcggcccga cctcgcaagc gccgcgtatc gacgaagcgc gcaacgattc gctgcgggaa
     1741 ctcgaagtgg cgttccgcga attagaacgg cgcctggagt cgcaccgtga cgattgcccg
     1801 ccctggctga tcgaccggct gctgcgccat ctgctcatcg acgtcaccgg caacacccat
     1861 cgcgccgagt tttgcatcga caaactctat tcgcccgaca gcgccacggg gcgcttgggc
     1921 ttgctcgaga tgcgcgcctt tgagatgccg ccgcacgcgc gcatgagctt gacccaacaa
     1981 ttgttgatcc gcgcgttagt cgcgcgcttt tggaaagtcc cgtatgcacc gccgcgcctg
     2041 gcacgttggg gcacgcagct gcatgaccgt tttctgctgc cgcattacat ttggctcgat
     2101 tttgccgacg tgatcagcga attgcatcaa ttcggctatg ccttcgatgc cggctggttt
     2161 gcgccgcact tcgaatttcg ctttcccaag tacggcgact ttgccgttcg tggtatcgag
     2221 ctggaattgc gcgccgccct cgagccttgg catgtcttgg gcgaggaggg cgcccccggc
     2281 ggcaccgcgc gctacgtcga ttcgtcgctg gaacgagtcc aagtcaaagt aaccgggctg
     2341 ccgcccgatc gctatgcgat cacctgcaat caagcgccgc taccgctgca agcgaccggg
     2401 accgccggcg agtacgtcgc cggagtgcgc tatcgcgcct ggcaaccgcc ttcggcgttg
     2461 cacccaacca tcgccgtgca caccccgctg acattcgata tcgtcgaccg ctggatgaaa
     2521 cggagcctcg gtggctgtca gtaccatgtc atgcatcccg gcggtcgcaa ctacgaggtc
     2581 tttcccgtca acgccttcgg cgccgagagc cgccgtctcg agcgcttttt tcgcatcggc
     2641 cactcaccgg ggatcatgga aatcggcgac gccggcattg atccggagtt tcccttcacc
     2701 cttgatttgc gtaaaattta atccggcgaa agtggcgaat gagttcaatt gacttagcac
     2761 ttggggaagt cgtagcggtt ttgcgggacg ttttcgctga atctactctt tctttttctc
     2821 gactgtgaac gctttgttaa attcttcatc attgtcgtaa gtcctgtcgc caccgtgttc
     2881 ggttatttcg caccggatgt aactaccgtc tttcaatttt ttgtatgcgt cgtaacagcc
     2941 ggggatatcg atggtttcaa taactaaacc gctggtggta cacatttcaa agaaagcatc
     3001 tgcatcaaag ccgtcttcag tgtgaactct ttcagcccat ttattagcta gcgtttccat
     3061 gcatagagtc taatcaagaa acacctttaa tcaaggtaat tcaaaaacaa gccgaccgaa
     3121 cgataagaca acgtgccgat ttcaccggca acatcaaacg gcccaatcat ttttcacgat
     3181 ccatcctggc aaatcgccca gcgtgtacct aactcggaac ccggccagct cgaaaagatt
     3241 gtggatgaac tcgctgcgct caggattttc ttcccattcc ttgcgcgttg gatatcggtc
     3301 cagtaatttt tcacaccaaa tgtctctcga tcctcgcgcc tctgcaaata gcttggccaa
     3361 ctcggtttcg atggcatgcg cttcgtttgg gtaagtatct ttaaaacttt tccccttaac
     3421 cgaccacctt ttcttccaga acgtttccac ggtgggctgt aatacattag aaggaaatcc
     3481 tacgcaagag acgctgatcc cggcgtttaa tttgacagtc tgtgcgctct ttggctgcta
     3541 gatccatcac gagcagcaca gtaagaataa atttatatcc tcacgcgaaa actacacgct
     3601 ttgaagaatt catgaataaa ccgctacgct caggatcttc ttcccattcc tgatgtttcg
     3661 acaacggctg aataactttt acacacccag gatcggtatc gtggttggca atagcgtctt
     3721 caac
//
LOCUS       DASQKS010000333         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2704964, whole genome shotgun sequence.
ACCESSION   DASQKS010000333 DASQKS010000000
VERSION     DASQKS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704964"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /gene="rplK"
                     /locus_tag="VE170_08675"
     CDS             <1..331
                     /gene="rplK"
                     /locus_tag="VE170_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HYJ15550.1"
                     /translation="VNIMEFCKAFNAATQAQLGMVTPVVITVYADRSFTFVTKTPPAS
                     VLLKKAAGLEKGSKEPGKTKVGKVTRAQVREIAELKLKDLTASDLAAAEKSVAGTARS
                     IGLEVEG"
     gene            374..1078
                     /gene="rplA"
                     /locus_tag="VE170_08680"
     CDS             374..1078
                     /gene="rplA"
                     /locus_tag="VE170_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HYJ15551.1"
                     /translation="MSATGKSYRKVLAKVDRNQRYQLEDSLKLVKETARAKFDETVDM
                     AIRLGVDPRQADQNIRGTVALPHGMGKTVRVLAFAKGEKEREAQEAGADFIGSDELIK
                     KISEGWLDFDKAVATPDMMAAVGRIGKILGPRGLMPNPKTGTVSLDIGKAVREIKAGK
                     LEFRVDKAGIVHVPVGRASFSADQLIDNARMVLLTILRAKPASAKGNYVKGVTIATTM
                     GPGIKIDLAQIRAMAA"
     gene            1141..1683
                     /gene="rplJ"
                     /locus_tag="VE170_08685"
     CDS             1141..1683
                     /gene="rplJ"
                     /locus_tag="VE170_08685"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYJ15552.1"
                     /translation="MSEAQAVRAGYGEKSEVVNAIHERLKSAKMAIITEYRGLTVAQM
                     TKLRREVRQVSGEYQVIKNTLVRRALKDTVYGDLEKLLEGPNGWVFAYDDPVLLSKTL
                     LKFAGDNDKLMIKGGVFEGRFMDAAEVKVLSQMPSKPELQAKLLATINAPATQLLRTI
                     QEPGAQMVRLLESLRKTKGE"
     gene            1718..2092
                     /gene="rplL"
                     /locus_tag="VE170_08690"
     CDS             1718..2092
                     /gene="rplL"
                     /locus_tag="VE170_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYJ15553.1"
                     /translation="MEGVSKEQVKDFIKNMSLMDAATLVKELEEELGVSAAAPVAVAA
                     AGPAAAAVEEKTEFNVVLAEAGEKKIQVIKVVRELTGLGLKEAKDLVDGAPKPVKEGV
                     SKADAESIKKKLEEQGAKVEIK"
     gene            2167..>3816
                     /locus_tag="VE170_08695"
     CDS             2167..>3816
                     /locus_tag="VE170_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYJ15554.1"
                     /translation="MAFQVANNLRFRRSFGRIRKIIELPYLIEIQKNSYELFLQKDAA
                     PTERQNMGLQEVFKSVFPIKDFNETASLEFVSYTLGEPKYDVEECHQRGMTFAAPLKV
                     TVQLVLWDVDQQTGARSIKNVKEQEVYFGEVPLMTINGTFMINGTERVIVSQLHRSPG
                     VFFEHDKGKTHASGKLLYSARVIPYRGSWVDLEFDPRDVVYVRIDRRRKFHATVLLRA
                     LGMTSEDLLNYYYRSDSIHFDGRKTTMEFNAAHLLGAKVSADVRDPKSNDIVVKEGKK
                     FTRQVLRQMEAARIKQVPIALEEIVGRVAAHDIVDPHGKEVLVECNHEITQEKLDLIR
                     DKGLTQIEVLFIDESNVGPYLRNTLLQDRIRSPQDAILEIYRRLRPGDPPTVESSTNF
                     FNNLFFNPDRYDLSKVGRLKLNHRLKLNIPLEQGTLRKEDILEVVRYLMELKNGHGSV
                     DDIDHLGNRRVRAVGELVENHFRVGLVRMERAIKEKMSLQDIETLMPQELINYKPVSA
                     ALKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRER"
BASE COUNT          967 a          935 c         1098 g          816 t
ORIGIN      
        1 cgtaaatatc atggagttct gcaaggcttt caatgccgcc acgcaagcgc agctcggcat
       61 ggttacgccg gtagttatca cagtctatgc cgaccggtca tttactttcg tcaccaagac
      121 gccgcccgcg tcggttttgc tcaaaaaggc cgcgggtcta gaaaagggct caaaggaacc
      181 aggcaaaacc aaggtcggaa aagtgacgag ggctcaagtc agggagattg cggaattgaa
      241 actcaaagat ctgacagcca gcgatttagc ggccgcggag aagtctgtcg cgggaaccgc
      301 gcgcagtatc ggattggaag tcgaaggtta gttcgcccag ccgtaaatta gctcagctaa
      361 accgaggaac gtcatgagtg caaccggaaa aagttatcgc aaggttttgg cgaaggtgga
      421 tcgcaaccag cgctatcagt tggaagatag tttgaagttg gtcaaagaaa ccgcccgcgc
      481 caaattcgat gaaaccgtcg atatggcgat tcgcttgggt gtcgatcccc gccaagccga
      541 tcagaacatc cgcggcacgg tggcgttgcc ccatggcatg ggtaaaaccg tccgtgtgtt
      601 ggcctttgcc aaaggtgaaa aagagcggga ggctcaagaa gccggcgctg attttatcgg
      661 tagcgacgaa ttgataaaaa agatcagcga aggctggctc gacttcgaca aggcggtggc
      721 gactccggat atgatggcag cggttggccg cattggtaaa atcctcggtc cccgtggtct
      781 tatgcccaat cccaagacag gtacggtgag tttggacatc ggtaaggcgg tccgggaaat
      841 caaggcgggt aagttggaat ttcgcgtcga caaagcgggc atcgttcatg taccggtggg
      901 tcgcgcgtca ttttccgccg accaattaat cgacaatgcc cgaatggtct tgttgaccat
      961 cctgcgcgcc aagccggcgt cggctaaggg caattatgtc aagggcgtca ctatcgccac
     1021 gacgatggga ccaggaatca aaatcgattt ggcacagatc cgggctatgg cggcctagtg
     1081 actcggggtc gttagcccgg ctttatcact gctagactag atttgaatca ggagaagaaa
     1141 atgtcggaag cgcaagcggt ccgtgccggt tacggcgaga aatcagaagt ggtgaatgcc
     1201 attcacgaac ggctaaagtc cgccaagatg gcgattatca cggagtaccg cggtttgacg
     1261 gttgcgcaaa tgacgaagtt gcgccgcgaa gtgcgtcagg tttccggcga atatcaagtg
     1321 atcaagaata ccttggtgcg gcgtgccctc aaagatacag tctatggtga tttggagaag
     1381 ttgctcgaag gccccaacgg ctgggtgttc gcctacgatg atccggtttt gctctccaag
     1441 actttgctca aattcgccgg cgacaacgac aagttgatga ttaaaggcgg cgtgttcgag
     1501 ggccgtttca tggatgcggc ggaagtcaaa gtcctgtcgc agatgccgag taaacccgaa
     1561 ttgcaagcga agctattggc gacaatcaac gcgccggcga cccagttgct aaggacgatt
     1621 caggaacccg gcgcccagat ggtgcgcttg ctcgaatcgt tgcgcaaaac caaaggcgaa
     1681 taacaattat ttagcaaaat tttaaagggg gaacgttatg gaaggcgtat cgaaagaaca
     1741 agttaaggat ttcatcaaga acatgagttt gatggatgcg gcgactctgg tcaaagagct
     1801 tgaagaggag ttaggtgtta gcgctgccgc tccggtagcc gtggcggcag ccggtccggc
     1861 agccgcggca gtggaagaaa aaactgaatt caacgttgta ctcgccgagg ccggggagaa
     1921 aaaaatacag gtcatcaaag ttgtccgcga actcaccgga ttgggtttga aagaagccaa
     1981 agatctagtc gacggcgcgc ccaagccagt gaaagagggt gtttccaagg ccgatgccga
     2041 gagcatcaag aagaagctcg aagaacaggg cgccaaggtc gaaatcaagt aattctctcg
     2101 cagacggtcg ttccgttgaa tgaattgaaa tggatcgtta accgctagtt cgagagagga
     2161 gcacgcatgg catttcaggt tgccaataac ttacgattcc gccgtagctt cgggagaatt
     2221 cgaaagatta tcgaactccc gtatttgatc gaaatccaga aaaattctta tgagctgttc
     2281 ctgcagaaag atgcggcgcc gaccgaacgc cagaacatgg gactgcagga agtttttaag
     2341 tcggttttcc cgattaagga ttttaacgaa accgcttctt tggaatttgt cagttatacc
     2401 ctcggcgaac ccaaatatga cgtcgaggaa tgtcaccagc ggggcatgac tttcgctgcg
     2461 ccgttaaaag ttaccgtgca gttggtgttg tgggatgtcg atcagcagac cggcgcgcgc
     2521 agcattaaaa atgtcaaaga gcaggaagtt tacttcggtg aagtcccgct catgacgatc
     2581 aatggcactt tcatgatcaa cggtaccgag cgggtaattg tcagccaatt gcaccgctcg
     2641 cccggagttt tctttgagca tgataaggga aagacccacg ccagcggcaa gctactctat
     2701 tcagcgcggg tgattcccta tcgtggttct tgggtcgatc tcgaattcga ccctcgggac
     2761 gtggtttatg tacgcattga ccgacgacgt aaatttcacg ctaccgtgtt attgcgcgcg
     2821 ttgggcatga ccagcgagga tttgctcaac tattattatc ggagcgattc gattcacttc
     2881 gacgggcgca agaccacgat ggagttcaat gccgcccatc tgttaggtgc taaggtcagc
     2941 gccgatgtcc gcgatccgaa atcgaacgat atcgtcgtta aagaaggcaa gaagttcacc
     3001 cggcaggtgc tgcgccaaat ggaagcggcg cggatcaaac aggtgcccat cgccctggaa
     3061 gagattgtcg gccgagtcgc ggcccatgac attgtcgatc cccatggcaa agaagtcctg
     3121 gtcgaatgca atcacgagat cacgcaggag aagctcgatc tgatccgcga caaaggactc
     3181 actcagatcg aagtactgtt tatcgacgaa tcgaatgtcg gtccttactt gcgcaacaca
     3241 ttgctgcaag atcgcatccg gtcccctcaa gatgccatcc tggagatcta ccggcgcttg
     3301 cggcccggcg atcctccgac ggtggaatcg tcgactaact tttttaataa cctatttttc
     3361 aatcccgatc gttatgacct atccaaggtc ggccgcttga agctcaacca ccggctcaag
     3421 ctcaatattc cgctggagca gggcacgttg cgcaaagagg atatcctcga ggtcgtgcgt
     3481 tatctgatgg aactcaagaa cggccacggc tcggtggacg atatcgatca cttgggtaat
     3541 cgccgcgtac gtgcagtggg tgaactcgtc gaaaatcact tccgcgtcgg cttggtgcgc
     3601 atggagcggg cgatcaagga aaagatgagc ctgcaggaca tcgagacctt gatgccccag
     3661 gagctgatca attacaagcc ggtgtcagcg gcgctcaaag agttttttgg ttccagtcag
     3721 ctgtcgcagt tcatggatca gaccaatccg ctgtcggaaa tcacccataa gcggcggctc
     3781 tcggccctcg gtccgggcgg tctaacccgg gagcga
//
LOCUS       DASQKS010000334         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5629703, whole genome shotgun sequence.
ACCESSION   DASQKS010000334 DASQKS010000000
VERSION     DASQKS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5629703"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VE170_08700"
     CDS             <1..582
                     /locus_tag="VE170_08700"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15555.1"
                     /translation="LDSPGIGLKWHPCSAPQFLAADAALQLKREHKIDIADVAKMAVS
                     IPPKRYARHYSAEVKTGLRGKFAINYVVALCFLDGKLEIATFTDEKVNRPEVQDAFNK
                     VQVICDESIPEPGPYCPVSVELKDGTRHSYTAKIAKGDPRNPMTEEEVTEKFCGNVKA
                     VLSEDRARNLVTTVRNLETVDNVQRLAGLLTPS"
     gene            644..1315
                     /locus_tag="VE170_08705"
     CDS             644..1315
                     /locus_tag="VE170_08705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ15556.1"
                     /translation="MGTTTQITARDGHTLDAYLARPANKPRGAIVIAQEMYGVTSYLR
                     DTCDFYAVQGYLTIAPALYDRRQRGLVLEYTQENHDLAQKIYKNWNWEQALDDLDAGK
                     NVVLETGKVAIVGFCWGGTLAWLAACRRDYAAAVAYYGSMMPDFAHETARCPVIAHIG
                     SADTTMPQERIELFHQAQPTVPINMYPGAQHGFDNPSRADRFHPEAGQLARERTVEFL
                     ARHVG"
     gene            1622..3742
                     /locus_tag="VE170_08710"
     CDS             1622..3742
                     /locus_tag="VE170_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYJ15557.1"
                     /translation="MASNQPLAPLFEADRLAIIGASDRNHYAVNVFKNLQNFGFDTSK
                     VVPINPGRPEVFGLKAYPSILDVPGELPLAVIAVNTQNVLAVVEDVGRKGVAAAVIFA
                     DGFAEGGEAGKKIQQDVTAAAKAANLKLLGPNCMGFVALRSKLGIWGGELPKNIRPGN
                     IGCIFQSSGMMNLLMNFGVKRGLGFHLCASGGNEIVLNSADYLDYQAECPEIDVIACY
                     FEAAPKEPERFAAALDRAIINGKAVLILRAGRTERAKKNVIAHTGQLAGSAAAWDACF
                     EQHGAIIVNDLDDLIDTTALFAGARLRPDQHERGVGLMTISGGDCTLLCDIADQEGLN
                     LPELSPATRQVMVEALDKPTVLGNPLDVEDLQRMKPEGFDRCLEKFFAEPNFDMLGVR
                     LNLPEAITPPAEKLYTQISELSKTNDKRVFVLTRASEPPAQVWYDKLTSLGLTFTGDY
                     RKSVRAMSRLRKNERDRAVGRFLPPTRSSTQPNRPSLSSGVASYRDTDKLLQSYGISL
                     APGLMVQSAGEAVLAAEKLGYPCVLKLASVDIPHKTEFGALRLGLETRIAVENAYAEI
                     LASVRIKKPDARIEGVLVQKQIKGVECLLGIARDEQLGPTLVMGLGGVFVEILADVQI
                     RIPPIGAAESKRALESLKGAKVFTGVRGAAAADLDALADMAAKLSWLAHDLRNEIAEF
                     DLNPVVVLPAGQGAFAVDALMVAR"
     gene            3867..4274
                     /locus_tag="VE170_08715"
     CDS             3867..4274
                     /locus_tag="VE170_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15558.1"
                     /translation="MSEEIVIEANPTPSLVNTTHLIYALHALSLLIGITTAATIIGAF
                     VFGLPSIIAVVINYLKRDEVRGTFLESHFRWQIRTFWFGLLWCLTGGFLFVTVIGIPI
                     ALMIVVAAGVWVIYRITRGWLALRDRKPMYANR"
     gene            4566..6308
                     /locus_tag="VE170_08720"
     CDS             4566..6308
                     /locus_tag="VE170_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYJ15559.1"
                     /translation="MEQHSLLSNIALVFVIATGFAFVAKFFKQPLILAYLVAGVVIGP
                     ELGLAWVQDKEAIELISEIGLILLLFIIGLEIDLKKLLSAGRTLLVTGISQFLLCVGL
                     GIGLAFLMGFTPGAGNFDWLYLAVTLALSSTLIVVKLLYDKFELTTLPGRITLGVLVC
                     QDIWAIVFLALQPNLHDPKLATLLESFVKGAGLVFLTLGLSRYLLPRLFSFAAKIPEL
                     MLIAALAWCFLVSGAAAELGLSREMGALIAGVGMSTFPYNIDVIAKVTNIRDFFVTLF
                     FVALGMQIPQPTLAIISGALVMSLFVLASRLLAVFPVLYGLGNGLRTSLIPSINLAQM
                     SEFSLVIASLGLALKHISAELVATLTFVFALTSILSTYLIAYNHEIQRQFARLLLKLG
                     FSNGVDNEDFGEKPGENAIVFLGCFREASSILHEIELHSNELKGDGVLGEILVIDFNP
                     QVLQELKRRGIKCLYGDIAHMDTLHHAHIHAARIVACTISDAILRGTSNLRLLQQARR
                     LCPDARVMVAADGIASAVDLYDQGADFVYIARLHSAYHMAELITRAVQDGEGFKLHCE
                     EEVRRLKQRVEVLQ"
BASE COUNT         1354 a         2001 c         1742 g         1367 t
ORIGIN      
        1 ctcgattcgc ccggcatcgg ccttaaatgg catccatgca gcgcgcccca gttcctcgct
       61 gccgacgccg cattgcaatt gaagcgtgaa cacaaaatcg acatcgccga tgtcgccaag
      121 atggcggtta gcattccgcc caaacgctac gcccgccact actcggccga ggtcaaaacc
      181 ggcctgcgcg gcaagttcgc catcaactat gtcgtcgccc tgtgttttct cgacggcaag
      241 ctggagatcg ctactttcac cgacgagaaa gtcaaccggc ccgaagtgca agacgccttc
      301 aacaaggtcc aagtcatctg cgatgagtcg attcccgagc ccggccccta ctgcccggtc
      361 agcgtcgaat tgaaggacgg cacccgccac agctatacgg caaaaatcgc caaaggcgac
      421 ccgcgcaacc cgatgaccga agaagaagtg accgagaaat tttgcggcaa cgtcaaagcc
      481 gtgctgtcgg aagaccgcgc tagaaatctc gtcaccaccg tgcgaaacct tgagactgtc
      541 gataatgtgc agaggctggc gggcttgctg acaccgtcat gagcagaatg ccgtagggtg
      601 ggtgaagcaa agcgataccc atcgccaaac tttgcaaaat aacatgggta caacgactca
      661 aatcactgcc cgcgacggcc ataccctcga tgcctatctg gcgcggccgg caaataagcc
      721 gcgcggcgcc atcgtcatcg ctcaagaaat gtacggcgtc acgagctacc tgcgcgatac
      781 ctgtgatttc tacgctgtcc agggctatct caccatcgca ccggcgctct acgaccgccg
      841 ccaacgcggc ctcgtcttgg aatacaccca agagaatcac gatctcgccc agaagatcta
      901 caagaactgg aattgggagc aggcacttga cgatctcgat gccggcaaga atgttgtctt
      961 ggaaaccggg aaagttgcca tcgtcggctt ctgctggggc gggacgctgg catggctcgc
     1021 cgcctgccgc cgcgattacg ccgccgccgt cgcctattat ggttcaatga tgccggactt
     1081 cgcacatgaa accgcgcgct gtcccgtcat cgcccacatc ggcagcgcag acacgaccat
     1141 gccgcaggaa cgtatcgaac tgtttcacca agcccagccg accgttccga tcaacatgta
     1201 tcccggcgcc cagcatggct ttgacaatcc gagccgcgcc gacagattcc acccagaagc
     1261 cggccagctt gcccgcgagc gcaccgtcga attcttggcg cggcacgtcg ggtgaaagta
     1321 gttggagtga tggagtactg gagtattggt tcggacgcga tccggctgat tgaatccagt
     1381 gacaccaagc aaacctgtca ttgcaaacat gtcatttcga gcacagcgag aaatctctcc
     1441 attaactacc tgcgtgttcc tcctgaagta agtgaaccta tctgagcatc gtccgagcta
     1501 accatcactc caatactcca ccactccacc actcctactc ttcctcctgc catagacaac
     1561 tttttccaac tgcgatagag cgaaatcgca ccagcaattt tttttatccc aggaggctct
     1621 catggccagc aatcaacctt tggcgccgct cttcgaagcc gatcgtctcg ccatcatcgg
     1681 cgccagcgac cgcaatcact acgccgtcaa tgtttttaag aatctgcaga acttcggttt
     1741 cgacacatcg aaagtcgtgc cgatcaaccc aggccgtccc gaagtcttcg ggttgaaggc
     1801 ttatccgagc atactcgacg ttcctggtga gctgccgctc gcggtgattg ccgtcaatac
     1861 ccagaacgtg ctcgccgtcg tcgaggatgt cggcagaaaa ggcgtcgctg ccgcggtaat
     1921 tttcgccgac ggtttcgccg aaggcggcga agcgggaaag aagatccagc aagatgtcac
     1981 cgccgcggca aaagcggcga acttgaagct cctggggccc aattgtatgg gcttcgtcgc
     2041 gctgcgctcc aagctcggca tctggggagg cgaactaccg aagaacatcc gcccgggcaa
     2101 tatcggctgc atttttcaga gcagcggcat gatgaattta ttgatgaact ttggcgtcaa
     2161 gcgcggtttg ggatttcacc tctgcgccag cggcggcaac gagatcgtgc tcaactccgc
     2221 cgactatctc gactaccagg ccgaatgtcc cgagatcgac gtcatcgcct gctatttcga
     2281 agccgcgccc aaagagccgg aacgtttcgc cgccgccctc gaccgcgcga taatcaatgg
     2341 caaagcggtg ttgatcctgc gcgccggccg caccgagcgc gccaagaaaa acgtcatcgc
     2401 ccacaccggc caactcgccg gctcggccgc ggcgtgggac gcctgcttcg agcaacatgg
     2461 cgcgatcatc gtcaacgatc tcgacgatct catcgacacc acggcgctgt ttgccggcgc
     2521 gcgattgcgc ccggaccaac atgagcgcgg tgtcggttta atgacgatct ccggcggcga
     2581 ctgtactctg ctctgcgaca tcgccgacca ggaaggtctc aatctgccgg aactgagccc
     2641 ggcaacccgg caagtcatgg tcgaagcgct ggacaagccg accgtgctcg gcaaccccct
     2701 cgacgtcgaa gacttgcagc gcatgaaacc ggagggcttc gaccgctgcc tggagaagtt
     2761 tttcgccgaa cccaacttcg acatgctcgg tgtccgtttg aatctgcccg aagcgattac
     2821 cccgccggcg gagaagctgt acacgcagat ctccgaattg agcaaaacca atgacaagcg
     2881 cgtcttcgtc ctcacccgcg ccagcgagcc gccggcgcaa gtgtggtacg ataaattaac
     2941 tagcctcggc ctgaccttca ccggcgacta ccgcaaatcc gtccgcgcca tgagccggct
     3001 gcgcaagaac gaacgtgacc gcgccgtcgg ccgcttcctg ccgcctacca ggtcaagtac
     3061 tcaaccgaac cgtccatcac tcagctccgg cgtcgcgtca tatcgcgaca ccgacaagct
     3121 gctccaatcc tacggcatct cgctcgcccc agggttgatg gtgcaatctg ccggtgaagc
     3181 ggtccttgcc gcggaaaagc tcggctatcc ctgtgtgctg aaactcgctt cagtcgacat
     3241 cccgcacaag acagaattcg gcgccctccg cctgggtctg gaaacccgta tagccgtcga
     3301 gaatgcttac gcggaaatac tcgccagcgt gcgtataaaa aagcccgacg ccaggatcga
     3361 aggcgtgttg gtgcaaaagc aaatcaaagg cgtcgaatgc ctgctcggca tcgcccgcga
     3421 cgaacaactc ggtccaacct tggtgatggg actcggcggc gtattcgtgg aaatcctcgc
     3481 cgacgtgcaa attagaatcc cgcccatcgg cgccgctgaa tccaagcgcg cgctcgagag
     3541 tttgaagggc gccaaagtct tcaccggcgt gcgcggcgcg gcggcggcag acctcgacgc
     3601 cctcgcggac atggccgcca agctctcttg gctggcccac gatctcagga atgagatcgc
     3661 cgagttcgat ttgaatccag tggtggtgct gccggccggc caaggcgctt tcgccgtcga
     3721 cgcattaatg gtcgcccgtt aacggctgaa attagtgaat cctgcgctac agcagttata
     3781 cgcagcaaac tggcggctgc atgggcttgt attagcgcta gtcgtcgagc aggatgagaa
     3841 tctgattcgc cgggaggcaa acactcatgt ccgaagaaat cgtaatcgaa gccaacccga
     3901 caccgtcgct ggttaatacc acccatctga tctatgcgtt gcacgccctc agcctgctga
     3961 tcggcataac caccgcagcg acgatcatcg gcgccttcgt cttcggcctt ccctcgatta
     4021 tcgccgtcgt catcaattac ctgaagagag acgaggtgcg cggcaccttt ctcgagtcac
     4081 actttcgctg gcaaattcgg accttttggt ttggcctcct ctggtgtctg accggcggtt
     4141 tcttgtttgt gactgtcata ggcattccga tcgcgctaat gattgtcgtc gccgccggcg
     4201 tgtgggtcat ctaccggatc actcgcggct ggctggcgct gcgggatcga aaaccgatgt
     4261 atgcgaacag gtgactacgg ctccggcact tttcccgcga agcacatttc agatcggcac
     4321 gctgatgccg attcaatcca acagtaaaac taaaacctct tcgagagaac caggatcgcg
     4381 gtctcgacaa ccggcctcgc cggagggtaa acctgggttg ccgttagtgc ccgactgagg
     4441 aaaagttgtg accgagacga tcctcaagta cgacttcgga tttcgttcaa gatttctccg
     4501 agcgggatgc ctgcgcgtcg tggtaccctc tcgctttttt tccgctttgc agtaattatc
     4561 tggcggtgga gcagcacagc cttctttcca acatagcgct ggtctttgtc atcgcgactg
     4621 gtttcgcgtt cgttgccaag tttttcaagc aacctttgat tcttgcctat ctggttgccg
     4681 gcgtcgttat cggcccagag ctcgggcttg cctgggtgca agacaaggaa gccatcgagc
     4741 tgatttccga gatcggcctg attctccttc tgttcatcat cggattagag atcgacctga
     4801 aaaagctgct gagcgccggt cggaccttgc tcgtcaccgg catcagtcaa tttctcctct
     4861 gtgtcggact gggcatcggc ttggcatttc tgatgggatt tacgccaggc gccggcaatt
     4921 tcgactggct ctaccttgct gtaaccctgg ccttgagcag cacgctcatc gtcgtgaagt
     4981 tgctctatga caaattcgaa ctcaccacct tgcccggccg gattaccctc ggcgttctcg
     5041 tgtgccagga tatctgggcc attgtctttc tcgcgctgca gccaaacctc cacgatccca
     5101 aactcgcaac cctcctcgag tctttcgtca aaggcgcggg gctggtgttc cttaccttgg
     5161 gtttgagccg gtacctgctg ccgcgcctgt tttctttcgc cgccaagatc ccggagctca
     5221 tgttgatcgc cgctctggcc tggtgttttt tggtgagcgg cgcggccgcc gagctggggc
     5281 tctctcggga gatgggcgcg ctcatcgccg gcgtcggcat gtctacgttt ccgtacaaca
     5341 tcgacgtcat cgccaaggtg accaatatcc gggatttttt tgttacgctt ttttttgtcg
     5401 cgctcgggat gcaaattcct cagccgactc tagcgatcat cagtggcgct ctggtcatgt
     5461 cgctttttgt cttggcgagc cgcttgctcg cggtttttcc ggtactctac ggactcggca
     5521 acggcctgcg aaccagtctg atcccgtcga tcaatctcgc gcagatgagc gaattttcgc
     5581 tggtcatcgc gtccttggga ctggctctaa aacacatcag cgccgagctc gtggcgacgc
     5641 tcactttcgt ctttgctctc acttcgattt tgtcgaccta tctaatcgcc tacaaccacg
     5701 agatccagcg ccagttcgcc cggctgctgt tgaagctagg tttctccaac ggcgtggata
     5761 acgaagactt tggcgaaaag ccgggagaaa atgccattgt atttctcggt tgcttccgcg
     5821 aagcgagctc cattcttcat gagatcgagc tgcactcgaa cgagctgaaa ggcgacggtg
     5881 tcctcggtga gatcttagtg attgacttca atcctcaagt gctccaagaa ctcaaacgac
     5941 gcggcatcaa atgtctctat ggagacatcg cgcacatgga cacactgcac catgcccata
     6001 tccatgcggc gaggatcgtt gcgtgcacga tctcggatgc gattttgcgc ggaacctcca
     6061 atctgcggct gctgcagcaa gcgcgccgac tttgtcctga cgcgcgggtc atggtggcgg
     6121 ccgatggcat tgcatccgct gtcgacctct acgatcaggg cgccgacttc gtgtacatcg
     6181 cgcgcctgca ttccgcgtat catatggcag agctgatcac gcgcgccgtc caagacggcg
     6241 agggattcaa actccattgc gaagaagagg tccgacgtct gaagcaacgg gtggaagttc
     6301 tccagtaacg aaacccagcg accggcggcg gcgacaaccg aacaaatgcg aaaccgaacg
     6361 caactcggct gggagattga ccccggcctt tcgacaaccc gccttcgtcg gcgtaggaac
     6421 tcctaatggc aaaagcgttc gccccgctga cagctacttt tctc
//
LOCUS       DASQKS010000335         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6075463, whole genome shotgun sequence.
ACCESSION   DASQKS010000335 DASQKS010000000
VERSION     DASQKS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6075463"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1595)
                     /locus_tag="VE170_08725"
     CDS             complement(57..1595)
                     /locus_tag="VE170_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ15560.1"
                     /translation="MSLRQQQIAVFCATVVVIFGLLGAGIWLIAQRLTAEATLQTALL
                     MARQVEIALADSLRERPVIVQRERQAQSAPASFWGFLGKVFDGRRSGPSSTTTTTRYS
                     APRHDEVRGLMRAFVDRSASIEAMWVVNGDGQLLYTSVQGDGDKSPSTALVMNKLRNG
                     ETLTSTKRQGNNDYYDVWVPLQMPQGVRSPGGLRLWLNPADWTGLLTGLWHQLTLLFV
                     LGGGVALVAAFLTTTLYTRRFRLIADTLRQAEAGTYATRPQYGSQDEVGVSLDLIDRL
                     VMKQRKNVGAPAPLQRLALAARTLAHEVKTPLNALAIHLEVLRNKVSTDDDSDGQSQR
                     SLTALDSSIRQVDRLVRDFTDYSAPVTMERKPIDVAAVLHTSLESIGAACQAKKINLL
                     KELAPGPWQVSGDAIRLRQTFDNLLRNAMEAQPDGGEVFVSAKAESAQLIIDISDGGP
                     GVPPDQRGEIFEFGKTTKATGSGIGLPLSQLIVESHGGALVYHERNGSGATFRVQLPL
                     ETIS"
     gene            1700..2710
                     /gene="moaA"
                     /locus_tag="VE170_08730"
     CDS             1700..2710
                     /gene="moaA"
                     /locus_tag="VE170_08730"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HYJ15561.1"
                     /translation="MLTDTFNRPLKDLRISVTDRCNFRCTYCMPLDTYEWIDKKEILT
                     FEEIARIAKLFVERGVEKIRLTGGEPLVRQDLHRLIEKLAPLEGLKDLCLTTNGALLA
                     DQVDALAKAGLKRVNVSIDTLDADKFKRMTKRGDLDKVLEGIFAAKAAGMTPLKLNAV
                     IERGVNDDDILPLVEFSRTNGFAMRFIEYMDVGNSNNWTSAKLVSKKQIIETIHARYP
                     LREIGRDQGSAPAVDYEFIDGQGDIGVIASVTEPFCSSCTRSRITADGKFVTCLFSDV
                     GHDLKAQLRSGASDDELGQFIAKIWSGRTDRYSADRLTALNSSNYDPKNHKKIEMISL
                     GG"
     gene            2889..3632
                     /locus_tag="VE170_08735"
     CDS             2889..3632
                     /locus_tag="VE170_08735"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ15562.1"
                     /translation="MILAEKIALVTGSTHNIGLAIARTFAREGAKVVVHSRHEADAKK
                     VAEEVGGDFFAADVADAEQVKAMFDHIKTKHGGLDILVNNVAHSSKGGILDITLEEWN
                     RILAINLTGYFLCIQHAGKMMKAQGSGSIINISAASGERSSAGGAAYSISKGAVNSLT
                     RQAAADLAPTIRVNGLISGLVGTPIGAKDMGSRKPEYDIIPLRRIGQPEDVAEAALFL
                     ASDKASYITNSILPVDGGRLNSMGSGSSR"
     gene            3653..>4638
                     /locus_tag="VE170_08740"
     CDS             3653..>4638
                     /locus_tag="VE170_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15563.1"
                     /translation="MGFVLSRDGWLLFATCGIRSLAYGFLSVILGLYLDAIGLSTTAI
                     GWIFTAALAGGAVMTIIITAFADSFGRKSLLIVGALLMALAGWAFAVSDNPIVLTFAA
                     VFGTISPSGKEVGPFLSLEQAILPQATSDRSRTAVFSAYNLVGSFAGALGALMVSLPS
                     KFSLTVVAGYNLLIWGYVAAALLLAGLFTLLSPEVEAPKKTQPTAQKLGLRHSRAIVA
                     KLAGLFALDAFAGGFIVQSIVAYWFYLRYQTDLQALGGIFFGTNIFAALSFLAAPAIA
                     RRIGLLNTMVFTHLPSNILILLVPLMPNVQWATAVLLLRYSLSQMDVPTRAS"
BASE COUNT          967 a         1344 c         1317 g         1010 t
ORIGIN      
        1 tggcgggtcg ccgggtcgtc atcgactacc agtacggcgg gacgtggacg tgccatttag
       61 ctgattgtct ccaagggtaa ttgcacgcga aaggttgccc cgctgccgtt gcgctcgtga
      121 taaaccagcg caccgccgtg ggattcgacg atcaactgac tcaacggcaa gccgatacca
      181 ctgcctgtgg ccttggtggt tttaccaaac tcgaagattt caccccgctg gtcaggcggc
      241 acgccggggc cgccgtcgct gatatcgatg atgagctgcg cgctttccgc tttagcactg
      301 acaaaaactt cgccgccgtc gggctgggct tccatggcgt tgcgcaaaag attatcgaaa
      361 gtctggcgca agcggatggc atcgccggaa acttgccatg gaccgggagc gagttctttc
      421 agtaaattta ttttctttgc ctgacatgcc gcccctatcg actccagact ggtgtgcaac
      481 actgcggcga cgtcgatcgg tttccgctcc atggttaccg gcgctgaata gtccgtaaag
      541 tcgcgtacca gacggtcgac ctgacgaatg cttgagtcca aagcagttag cgagcgctgg
      601 gattgcccat cactgtcatc atcggtacta actttattgc gcaggacctc aagatggatg
      661 gccaaagcgt taaggggggt tttgacttca tgggccagcg tccgtgcggc gagggcgagc
      721 cgctgcagcg gcgcgggcgc gccgacgttt ttgcgctgct tcatgaccaa gcgatcgatc
      781 aagtccaaac taacaccgac ttcgtcctgg ctaccatact gtggccgggt ggcgtaggtg
      841 ccggcctcgg cttggcgcaa ggtgtcggcg atcaaacgaa aacggcgggt ataaagcgtc
      901 gtggtcagaa acgccgccac cagcgccacg ccaccgccga gaacaaacag caaagtcagc
      961 tgatgccata ggccggttag cagcccggtc cagtcggccg ggttaagcca gagccgcagc
     1021 ccgccaggac tgcgcacgcc ttggggcatc tgcaggggca cccagacatc gtaatagtcg
     1081 ttattgccct ggcgtttggt gctggtgaga gtttcgccgt tgcgcaattt attcataacc
     1141 agagccgtcg atggcgactt atcgccatcg ccctgaactg acgtgtaaag caactgcccg
     1201 tcgccgttga ccacccacat ggcttcgata ctggcgctcc ggtcgacgaa ggcgcgcatc
     1261 aggccgcgga cctcgtcatg gcgcggtgcg ctatagcggg tggtcgtcgt ggtcgaggat
     1321 ggcccggagc ggcggccatc gaacacctta ccgagaaaac cccagaatga tgccggcgca
     1381 ctttgtgcct gtcgctcgcg ctgaacgatc accgggcgct cgcgcaaact atcggctaga
     1441 gcgatctcga cttgacgcgc cattaacaaa gccgtttgca aggtcgcttc ggcggttaaa
     1501 cgttgggcga ttagccagat accggcgccg agtagaccga aaataaccac cacggtggcg
     1561 caaaagacgg cgatttgttg ctggcgcagg ctcattgccg aattaggata acagatcggc
     1621 caaatccgac aaagcaatga tttttcagca aaaattcgcc agcttgaacc ccctacccct
     1681 ttgttgcata gttatttcga tgctcaccga cacattcaat cgaccgctca aggacctgcg
     1741 catttcggtc accgaccggt gtaacttccg ctgcacctac tgcatgccgc tggacacata
     1801 cgagtggatc gataagaaag agattttgac gttcgaagag atcgcccgca tcgctaagct
     1861 atttgttgag cgcggcgtcg aaaagattcg cctcaccggc ggcgagccgc tggtgcgcca
     1921 agacctgcat cgattaatcg aaaaactcgc gccgctggaa ggcctcaagg atctttgttt
     1981 gactaccaat ggcgcgctgc tggccgatca agtcgacgcc ctcgccaaag ccggactgaa
     2041 acgggtgaat gtcagcatag ataccttgga tgcggacaag ttcaaacgca tgaccaagcg
     2101 aggcgatctc gacaaagtgc tcgagggaat ctttgccgcc aaagcggctg gtatgacgcc
     2161 gctcaaactc aacgccgtga tcgagcgcgg cgtcaacgac gacgatatct tgccgctggt
     2221 ggaattctct cgcaccaacg gctttgccat gcgcttcatc gagtacatgg atgtcggcaa
     2281 ctcgaacaat tggacttcgg cgaaactggt gtcaaaaaaa cagattatcg aaacgatcca
     2341 cgcccgctat ccgctgcggg aaatcggccg cgaccaaggc agcgcgccgg cggtggacta
     2401 tgaattcatc gacggccagg gcgatatcgg cgtgatcgct tcggtgaccg agccgttttg
     2461 ttcaagctgc acgcggtcgc ggattactgc ggacggtaaa ttcgtcacct gcttattttc
     2521 cgatgtcggt catgacttga aggcccagct acgcagcgga gccagcgacg acgagcttgg
     2581 ccagtttatc gcgaagatct ggtccgggcg caccgaccga tattcggccg atcgtctgac
     2641 agcgctcaat tcgtcgaact acgatcccaa aaaccataag aaaatcgaaa tgatctcgct
     2701 cggcggatga tgcagtttcg ccggattggc ggctacggga tcgttgaccg ctgccgctac
     2761 tgatttgtcc agattaaact tacccagcag gccgcgacgc tctaggttgg cgtcgctcat
     2821 cggctgattg ctcgccgttg tggttaaaat agctactgac tttcaatgaa attccgaagg
     2881 agcgttgcat gattctcgcg gagaaaatcg cattagttac cggctccact cataatatcg
     2941 gcctagctat cgcccgcaca ttcgcccgcg aaggcgctaa agtcgtggtg cattcgcgcc
     3001 atgaagcgga cgccaaaaaa gttgccgagg aggtcggcgg cgattttttt gcagccgatg
     3061 tggccgatgc cgaacaggtc aaagcgatgt tcgatcatat aaagacaaaa catggcggtc
     3121 tggatattct cgtcaacaac gtcgcccact cgagcaaagg cggtatcctc gacattacct
     3181 tggaagaatg gaaccgcatt ctggcgatca atctgaccgg ctactttctc tgcattcaac
     3241 atgccggcaa gatgatgaaa gcgcagggca gcggcagcat aatcaatatt tccgccgcct
     3301 cgggcgagcg ttcgagcgcc ggcggcgccg cctattcgat ttctaaaggc gcggtgaatt
     3361 cgctcacccg acaagccgcc gccgacctgg caccgacgat ccgcgttaac ggtttgattt
     3421 ccggtctggt cggcacgccg atcggcgcca aggacatggg cagccgcaaa ccggaatacg
     3481 acatcattcc cctgcgccgt atcggacagc cggaggatgt cgccgaagcc gcgctgtttc
     3541 tcgcctcgga taaggctagc tacatcacca actcgatctt gccggtggac ggcggccggc
     3601 tgaattccat gggcagcggc agctcgcggt gaactgagcc aacgcgagtt ccatgggctt
     3661 tgtgctttcc cgggatggct ggttattatt cgccacctgc ggcatacgtt ccttggccta
     3721 tggttttctc tcggtcattc tcggactcta tctcgatgcc atcggcttaa gcacgacggc
     3781 gatcgggtgg atatttaccg cagcccttgc cggaggcgcg gtaatgacga ttatcatcac
     3841 ggcttttgcc gacagctttg ggcgaaagag tttgctgatc gtcggcgcat tgctgatggc
     3901 actcgcgggc tgggcatttg ctgtcagtga caatccgatc gtgctgacct tcgcggcggt
     3961 cttcggcacc atcagcccgt cgggaaagga agttgggccg tttctttcac tcgaacaagc
     4021 gattttacca caagccacca gcgatcggag ccgcaccgcg gtgttctcgg cgtacaattt
     4081 ggtcggctcg tttgccggcg cactgggggc gctgatggtc agtttgccct cgaaattttc
     4141 attgaccgtg gtcgccggtt acaacttgtt gatctggggt tacgttgccg cggcgctttt
     4201 acttgcggga ctgttcacct tgctgtcgcc tgaggtcgaa gcgccaaaga aaactcaacc
     4261 gacggcccaa aaattaggct tgcggcactc ccgcgccatc gtcgccaagc tcgccggatt
     4321 gttcgctctc gatgctttcg ccggcgggtt catcgttcag agcatcgtcg cttactggtt
     4381 ttatctgcgt taccaaactg acttgcaggc tctgggcggg atcttcttcg gcaccaacat
     4441 tttcgctgcc ctgtcctttc tcgccgctcc ggcaatcgct cggcgcatcg gtttactcaa
     4501 caccatggtg ttcacccacc tgccgtcgaa tatcctaata ttgctggtgc cgctgatgcc
     4561 caacgtacaa tgggccacgg cggttttact gctgcgttac tcgctctcgc aaatggacgt
     4621 gccgacccgc gcttctta
//
LOCUS       DASQKS010000336         6545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3957556, whole genome shotgun sequence.
ACCESSION   DASQKS010000336 DASQKS010000000
VERSION     DASQKS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957556"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1051)
                     /locus_tag="VE170_08745"
     CDS             complement(302..1051)
                     /locus_tag="VE170_08745"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15564.1"
                     /translation="MKLKDKVAVVTGAAQGIGRATALMMGREGAKLIVADLQAEKAQA
                     VANELMALGAEALALEVNVANEVSVKRMARSAIERFGRADILVNVAGIYFPKASVVDL
                     TEEDWDRTIDINVGSNFLCCREFVPGMRAQKSGRIISLASGIGHYGMRQFSHYAASKA
                     AVMGFVKALAREVGPDGITVNAICPGSANTSMPRQHRSEEEVMARLSATPLPHILEPE
                     DIAGSIVFLASDAAKFITGQSYNINSGTYMF"
     gene            complement(1048..1791)
                     /locus_tag="VE170_08750"
     CDS             complement(1048..1791)
                     /locus_tag="VE170_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15565.1"
                     /translation="MTQVALITGGGGGIGGAVAQRLAAAGITVVIADYDGGAAERVKG
                     DIVAAGGKADAMAIDVTLAREVKAVVGDVSKRFGRIDILANIAGGSAYTKRIEEFTWA
                     EWKEVMDANLKGTFLMCREVAPLMQRQKSGRIINTASNYGVTGSALRTPYAAAKAGII
                     TFTKSLALELAPDLVLANTVAPGPTDTPRVMEKESPEARRQRWWSQIPLGRTALPEDL
                     AEMYYFLTTPESAAITGQTFHVNGGVVMP"
     gene            complement(1793..3562)
                     /locus_tag="VE170_08755"
     CDS             complement(1793..3562)
                     /locus_tag="VE170_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ15566.1"
                     /translation="MSEPPPSAHSRHVGQIFFQRAAELGGRTFIKLQTGESFEEVSWR
                     EFGILVGQVLLGLCAVGIAPGETVAIVGENCLPWLAADLATLAGGFPNVVLAPSLSDG
                     MFVKVLGHAQCRAAFVENAAVARRLVNLQRQLPALQRLIVMDDSGAGIVGALNFRQLL
                     ERGAGADGLAARLQAVQANDLATIMYTSGSTGEPKGVMRSQDNLLANITNGGPITVSA
                     PDELSVNILSLNHLFGRFGFLKSAVTGRTTALIEAADTKVDLPVIEQLSPTGLALVPR
                     IMERIWQRLLAQDDNQECWRILERLDAERFKAGLSDADSRCFDALRAQLGANVRRALG
                     GRIKYVAYGGAAMPPRIMRFFELIGLPLIGSYGSTECGGVTLCGIGDSRPGNLGKPFA
                     NVQIRIAADGEILVRGSTVSPGYFKNPEATREAFNEEGWYYTGDIGLIEADGSLKITG
                     RKKDIFNCADGSNIYPGFIELQLENEPYIGQAVLVGDRRPFIAALIFPDGQKIAESLS
                     RDIATLSETEITAALSSQIERVNGRLESYERIRRIAVVPSGFPADVRSINHFHKVKVN
                     REQVAARYRLQIDAIYRTALQED"
     gene            complement(3559..4926)
                     /locus_tag="VE170_08760"
     CDS             complement(3559..4926)
                     /locus_tag="VE170_08760"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HYJ15567.1"
                     /translation="MDSATNGVTFSVPYEMAVVRPLRQTTEPERQAALANAHYNTDII
                     PQELIYIDLSTDSGVSASSTGQLAAVAQSVEPGMGLASEGSAAYGRLAAALQRFFGFD
                     YFVPTTQGRSAERIWTKINIKPDSIVAGNLLFPSTRNHIGMAGAQIVDVIGEAAHDFV
                     TDDPFKGNIDLNKLEQLVQEQGAGRVSCIYVELSVNSCGGHPVSLGNLSAVKAIAARH
                     RIPLFVDACRILENSAMIKAREAAYRQRSLAEIVKETCALADGVTMSALKDFLVPTGG
                     FIFTRDRSSQQKAAMQSFLDGVQLSAGAMDAISATLPEIFASDAYMTQRIDQVKYLWD
                     KLAGTVPVLRPASGHGVFIDLKKFFAPIVPEPFAAEALAAFVYLKSGIRISKGPPLAP
                     SQVARDVELLRLAVPARKYLNGHMDDVANSVSYAFARRNEIRKLKKIEDPARSKYDPA
                     HFVTL"
     gene            complement(5035..5844)
                     /gene="cutA"
                     /locus_tag="VE170_08765"
     CDS             complement(5035..5844)
                     /gene="cutA"
                     /locus_tag="VE170_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HYJ15568.1"
                     /translation="MSELIVVYVTVGSGEEADRMAQALVGEQLAACVNRVRSIQSVYR
                     WQGKVEQSDEELLIIKTRRQLFPALEKRVKELHSYSVPEIIALPILEGSADYLTWLRE
                     QALAKPPQDENIRTVREVSAGGVIYRRSGDLLEVALIHVRNRWGLPKGHVEEGERVEE
                     AALREVREETGLEGKLVRKLGDIRYSYRDKTKESEPVRIYKRVHFYLMRYVRGDVRDH
                     DHESDDARWFPIEQAMKQLKFATERKMVHRAMTMLTVRPAEPPAPAVTSGS"
     gene            6002..>6545
                     /locus_tag="VE170_08770"
     CDS             6002..>6545
                     /locus_tag="VE170_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYJ15569.1"
                     /translation="MKTKDELRQQVWQALQTARVARFPGAIGRIPNFIGAEACAKQLA
                     ATVYWKAAKIVKINPDSPQRAIRQRALAEDKIIYMPVPRLRDAKPFIELDPKKLQCSP
                     YAASSIKGAGLYGRPVTLDEIKGIDLVVCGSVAVNRAGARVGKGGGYSDLEFALLTEA
                     RKIGATTAIVTSVHPRQIVNH"
BASE COUNT         1299 a         2038 c         1901 g         1307 t
ORIGIN      
        1 gggcaaacca ttcagccggc ctggaagcgc ggattggaat tggctcctgg cgaggtgtaa
       61 agaatccagc tcggaggagt aagaccaaaa ttccgaggca tgatttttca gcctgtcact
      121 gcggtccggc agggtttcga ggttcttcgc acttccattc acgatctttg gcgcggctta
      181 ggtgaatttc accatgcaaa aaagccgaga gagcgggttt tcaacccggc cctgcccccg
      241 tcgattctgt ttgcgccctt tgcgttcctt gcggctattc gtcttttcgg ttgcggcatc
      301 atcagaacat gtacgtgccg ctgttgatat tataagactg tccggtgatg aacttcgccg
      361 cgtcgctggc gagaaagacg atggagccgg cgatgtcttc gggctcgagg atatgcggca
      421 gcggtgtcgc gctcagccgc gccatcacct cttcttcgga gcggtgctgg cgcggcatgg
      481 acgtgttggc cgacccggga cagatcgcgt tgacggtgat gccgtccggt ccgacctcgc
      541 gcgccagcgc tttgacgaat cccatcaccg cggctttcga ggcggcgtag tgcgagaact
      601 ggcgcatgcc gtagtggccg ataccggagg cgagagaaat aatccggccg cttttctgcg
      661 cgcgcatccc tgggacgaat tcgcggcaac agagaaaatt gctgccgacg ttgatgtcga
      721 tcgtgcgatc ccagtcttcc tcggtaaggt cgaccaccga ggccttgggg aaataaatcc
      781 cggcgacgtt gaccaatatg tcggcgcggc cgaaacgctc aatggcgctg cgcgccatgc
      841 gcttgaccga aacctcgttg gcaacgttga cttccagcgc cagcgcctcg gcgcccaacg
      901 ccattaactc attggccacc gcttgggctt tttccgcctg caggtcggcg actattagtt
      961 tcgcgccttc gcgtcccatc atcagcgccg tcgcccggcc gatgccctgg gccgcgccgg
     1021 ttacgaccgc gactttgtct ttgagtttca tggcatcacc accccgccgt tgacatgaaa
     1081 ggtttgtccg gtgatcgccg cgctctccgg cgtggttaga aaataataca tctcggccaa
     1141 atcctccggc agcgcggtgc gaccgagcgg gatctgcgac caccaacgct gtctgcgcgc
     1201 ttccggcgat tccttttcca tgacccgcgg cgtatcggtc ggccccggcg cgacggtgtt
     1261 cgccagcacg agatccggcg ccagttccaa cgccagcgat tttgtgaagg taatgatgcc
     1321 ggccttggcc gccgcgtagg gcgtgcgcag ggccgaaccg gtgaccccgt agttcgacgc
     1381 cgtgttaatg atccggccgc ttttttgtcg ctgcatgagc ggcgccactt cgcggcacat
     1441 gagaaacgtg cccttcaagt tggcgtccat gacttccttc cattccgccc aggtgaactc
     1501 ttcgatacgc ttggtgtagg cgctgccgcc ggcgatgttg gcgagaatat cgatgcgtcc
     1561 aaagcgtttg gacacgtcgc cgaccacggc tttcacttct cgcgccaagg tcacatcgat
     1621 cgccatggca tcggccttgc cgccggccgc gacgatgtcg cctttaaccc gctccgccgc
     1681 gccgccatcg taatcggcga tgacaacggt aattcccgcc gccgccaggc gctgcgccac
     1741 cgcgccgcca atgccgccgc ccccgccggt gatcaaggca acttgagtca attcaatcct
     1801 cctgcaacgc cgtccggtaa atggcgtcga tttgtagtcg ataacgcgcc gcgacctgtt
     1861 cgcggttcac tttgactttg tgaaaatgat tgatgcttct aacgtcggcg ggaaacccgc
     1921 tcggcacgac cgcgatccgg cgaatccgtt cgtaggattc gagccggccg ttgactcgct
     1981 cgatctggct cgacagtgcc gcggtgattt ccgtctccga caaagttgct atatcacgac
     2041 tgagagattc ggcaatcttt tggccgtcgg ggaatatcaa tgccgcgatg aacggccgcc
     2101 ggtcgccgac taacaccgcc tggccgatat agggctcgtt ttccaattgc agctcgatga
     2161 aaccgggata aatgttcgag ccgtccgcac aattgaaaat atctttcttg cgtccggtga
     2221 tcttgagcga accgtcggct tcgatcagac cgatatcccc ggtatagtac caaccctcct
     2281 cgttgaacgc ctcgcgcgtc gcctcgggat tcttgaagta acctggcgag accgtcgaac
     2341 cgcgcacgag aatttcaccg tcggcggcaa tgcgaatctg gacattggca aacggtttgc
     2401 ccaaattgcc ggggcgactg tcgccgatac cgcaaagggt cacaccgcca cactcggtgg
     2461 agccatagga gccgatcagc ggcaaaccga tcaattcgaa aaagcgcatg atgcgcggcg
     2521 gcatggccgc gccgccgtag gcgacatatt tgatccggcc gccgagggcg cgccgcacgt
     2581 ttgcaccgag ctgtgctcta agcgcgtcga agcagcgact gtcggcgtcg ctcaaaccag
     2641 ccttaaatct ctcggcgtcg agtcgttcga gaattcgcca gcattcctga ttatcgtcct
     2701 gagccaatag ccgctgccaa atccgctcca tgatccgcgg caccagcgcc aagccggtgg
     2761 gcgaaagctg ctcgatcacc ggcaaatcca ccttggtgtc cgcggcttcg atgagcgccg
     2821 tggtgcggcc ggtcaccgcg cttttgagaa acccgaaacg gccgaacaaa tggttcaagc
     2881 tcaggatatt gacgctcaac tcgtccggcg cgctaaccgt gatcggcccg ccattggtga
     2941 tattggcgag cagattatcc tggctgcgca tcaccccttt gggttcgcca gtggaaccgg
     3001 aagtgtacat gatggtcgcc aagtcgttgg cctgcaccgc ttgcagtcgc gccgccaagc
     3061 cgtctgcgcc ggcaccgcgt tcgagcagct ggcgaaaatt gagcgcgccg acgatcccgg
     3121 cgccgctgtc gtccatcacg atcagccgtt gcagagccgg caattgcctc tgaagattga
     3181 ccaggcggcg ggcaaccgcc gcattctcga caaaggcggc ccggcactgc gcatggccga
     3241 gcactttgac aaacatgcca tcggaaagac tcggcgccag gacaacgttg ggaaaaccgc
     3301 cggcaagagt cgccaggtcg gcggcgagcc agggcagaca attctcaccg acgatcgcga
     3361 cagtttcacc cggtgcgata ccgacggcgc ataagcccaa cagcacctgg ccgaccagga
     3421 tgccgaactc gcgccaggaa acttcctcaa acgattcgcc ggtttgtaac ttgataaacg
     3481 tccggccgcc caactcggcg gcccgttgga aaaagatttg gccgacatgg cggctgtgag
     3541 cagacggcgg aggctcgctc atagagtaac aaaatgggcg ggatcgtatt tcgatcgcgc
     3601 gggatcttca atttttttca attttctgat ttcgttgcgc cgcgcgaaag cgtagctgac
     3661 cgaattggca acgtcgtcca tgtggccgtt taaatacttc cgcgccggca cggcgagacg
     3721 caagagttcg acatcccgcg ccacctggct cggcgccaac ggcggccctt tggagatgcg
     3781 aatgccgctc ttgaggtaaa cgaacgccgc caaagcttcg gcggcgaatg gctcaggtac
     3841 gatcggcgca aaaaacttct tgagatcgat aaacacgcca tgaccgctcg ccggccgtag
     3901 caccggcacg gtgccggcga gtttgtccca gagatatttc acctgatcga tccgctgcgt
     3961 catataagca tcgctggcga agatttccgg caaagtagcg gagatagcat ccatggcgcc
     4021 ggccgagagc tgaacgccgt cgagaaaact ctgcatcgcc gccttttgct ggctgcttcg
     4081 gtcgcgggta aagataaaac cgccggttgg taccaggaaa tctttaagcg cactcatcgt
     4141 gacgccatcc gccaaggcgc aggtttcctt gacaatctcg gcgagtgacc gctgccgata
     4201 ggccgcttcc cgcgctttga tcatcgcact gttctcaaga atgcggcagg cgtcgacaaa
     4261 gagtggaatc ctgtggcggg cggcgatcgc tttgacggcg cttagattgc ccagcgaaac
     4321 cggatggccg ccgcaggagt taacgctgag ctcgacgtaa atacagctaa cccgtccggc
     4381 accctgctcc tgaaccaact gttcgagttt attgagatcg atattgccct tgaacgggtc
     4441 gtcggtaacg aaatcatggg cggcctcgcc gatcacatcg acaatctgcg ctccggccat
     4501 tccgatatgg ttgcgcgtcg acggaaacag cagattgccg gcgacgatgc tatccggctt
     4561 gatattgatc ttggtccaga tgcgttccgc cgagcgtccc tgggtcgtcg gcacgaaata
     4621 gtcaaatccg aaaaaacgct ggagtgccgc cgccagccgg ccgtaggcgg cgcttccctc
     4681 ggacgccaac cccattccgg gctcgacgga ttgcgccacc gccgcaagtt gcccggtgct
     4741 gctcgcgctg acgccgctat cggtggacaa atcgatataa atgagttcct ggggaatgat
     4801 gtcggtgttg taatgcgcgt tggcgagtgc ggcttgccgc tccggctcgg tggtctggcg
     4861 cagcggccgc accaccgcca tctcgtaagg cacggaaaat gtcacgccgt tcgttgcact
     4921 atccattgtc gggcactccg cggtaacacg cagcccttac gtaacatgaa atcggcgccc
     4981 tgaaaataag aaattgaaag tgaaccggcc gccgggcgaa ccgttgggtg gcagttacga
     5041 gccgctggtc acggccggcg ccggcggctc cgccgggcgc accgtcagca tggtcatggc
     5101 gcgatgcacc atcttgcgct cggtggcgaa cttgagctgc ttcatcgcct gttcgatggg
     5161 gaaccagcgg gcatcatccg actcatgatc gtggtcgcgc acatcgccgc gcacatagcg
     5221 catgagatag aaatgcaccc gcttgtaaat acggacgggc tcgctctctt tggtcttgtc
     5281 gcgataagaa taacgaatat cgccgagctt gcgcaccagc ttgccttcca aaccggtctc
     5341 ttctcgcacc tcgcgcagcg ccgcctcctc gacccgctcg ccttcctcga catgcccttt
     5401 gggcagaccc cagcgattgc gcacatggat gagcgccacc tccaacaggt cgccgctgcg
     5461 ccggtagatc acgccgccgg cggaaacctc tcgaaccgtg cgtatgtttt cgtcctgcgg
     5521 cggcttcgcc aacgcttgct cgcgcagcca ggtcaggtag tcggcgctgc cctcgaggat
     5581 cggcagcgcg atgatctcgg gaaccgaata gctgtgcagt tccttgacgc gcttttccag
     5641 cgccgggaag agctggcggc gggttttgat aatcagcagc tcttcgtcac tctgctcgac
     5701 cttgccctgc caacggtaaa ccgactgaat cgagcgcacg cggttgacgc aagcagccag
     5761 ctgctcgccg accaacgctt gagccatgcg gtcagcctct tcacccgatc ccaccgtgac
     5821 atagactacg atcaattctg acatcaacgg caatcttata actcagcccg ataggcagtc
     5881 aatctccggg aaaaacaggc tggcattggg ccctccggcg tagatcactc cggccacggc
     5941 tttacggtac ttgctgttac cctattgcct gctgccgagt gcattattag gttgaaacgt
     6001 aatgaaaacc aaagacgaac tgcgccagca ggtctggcaa gccttgcaaa ccgcccgagt
     6061 cgcgcgtttc ccgggagcga tagggcgcat tcccaacttc atcggcgctg aggcctgcgc
     6121 caagcaactg gcggcaacgg tctactggaa agccgctaaa atcgtgaaaa tcaatccaga
     6181 ttcgccccag cgggcgattc gtcaacgggc cttggccgag gacaagatta tctacatgcc
     6241 ggtgccgcgg ctacgcgacg ccaagccgtt tatcgagctc gatccgaaaa agcttcaatg
     6301 ctcaccctac gcggcgtcgt cgatcaaggg tgccgggctc tatggccggc cagtaacgct
     6361 cgatgaaata aaaggaatcg atctggtggt ctgcgggtcg gtggccgtca accgcgccgg
     6421 cgcccgggtc ggcaagggcg gcggctattc cgacctggag ttcgccctgc tgacggaagc
     6481 gagaaagatc ggcgccacca ccgccatcgt cacatcggta catccccggc aaatcgtcaa
     6541 ccatg
//
LOCUS       DASQKS010000337         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3543860, whole genome shotgun sequence.
ACCESSION   DASQKS010000337 DASQKS010000000
VERSION     DASQKS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3543860"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VE170_08775"
     CDS             <1..782
                     /locus_tag="VE170_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15570.1"
                     /translation="TKVLGLDLLERRDREAVLKHPNTGWKLIVHEGGAEVKDKPERNH
                     YGVRVANNLEVDHAYQYLLANKERLSLTKVVKRKERAGSYSMFFVEPGGNYWEIESYE
                     NRHKAGLPYEVAYPWKTALSEERLPGCGYVPQAMTHGTMECTDLQRSKKFYQQGLGLD
                     VITHVPTIEPHDIKHPTTPWYVVSLEVPPKNKHYLTPLQRYTVAVESSAALTEANRQF
                     TERRDEFGLCAIEAVQDSRAGQSFQICDLDRNWWEVAYIAN"
     gene            787..1815
                     /locus_tag="VE170_08780"
     CDS             787..1815
                     /locus_tag="VE170_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15571.1"
                     /translation="MELSNPSLIRPIGPIRLIFAAALLLTLTALAAAQPIKLNVAYTS
                     TTTNFSIAWVAKLEGIYRKHNLDVELILMQGPSTYLPALLSGNIHVLYGGGTAVSRAI
                     AAADPNIIVIGSETRYVPLRLMVTPSIKSAADLKGKRLGVGRAGLDEYATLYYLEKNN
                     LVPNKDVALIYTTGGILARAAQMKQGLYDGMAIPPPNEIELEKQGFRELAYFFDLRMS
                     YAGIPYTVTRDFRDKNQRVLTDFMTAITEAIQLYRMNKEVGYRSIVQITRQNDPMVLE
                     KTYRANLQQYDAIQGLPFPWQDGIESMINGFHARFTPALIKNRDARPYLDPSFVQRAV
                     ERLGLVKK"
     gene            1830..2729
                     /locus_tag="VE170_08785"
     CDS             1830..2729
                     /locus_tag="VE170_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15572.1"
                     /translation="MSDTAKPAVASLGITKGHYECNYLDQTVPVLSELLALEVVAKGD
                     REVTMKHPNTDWLLVVHESGPAVPDKPFRNHYGVRVKNNEQVDRAYEYLMVKKEQLAL
                     KKVVMRNERAGSYSVFFVEPGGNYWEIESYEHRHQAGLPYEVSYPWQTELSEAQFPGR
                     GYLPHGFTHGTIECNDWASSVKFYKDGLGMDVITHVQTTKPHNIKHPANSWYVVSLEV
                     PEKSRKYLSPLQRFTIAVASVDELREARENLSARRNEFAITEIGDIQDTLDGKAFMLC
                     DLNKNWWEIAAPLRHSRDSGNPG"
     gene            2756..3385
                     /locus_tag="VE170_08790"
     CDS             2756..3385
                     /locus_tag="VE170_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HYJ15573.1"
                     /translation="MNVHRIDNKKTALLFFDMLNVYFHGIPDEKHKALKPVVANAVRL
                     MNAARQNGILIYYAMANHRPDGESRYRAITDTDMRLRPWPNDDCNPTVHGATEGSREQ
                     KVLDEIAPQPQDYIIPKYRWSTFHQTYFDLALRSRGIDTIIISGGAVDVGVASTVYSA
                     RDHDYNLVIVRDACSNSHDDSMAALMNTVFPRLARVRSTDQVLDMIRTG"
     gene            3455..>3802
                     /locus_tag="VE170_08795"
     CDS             3455..>3802
                     /locus_tag="VE170_08795"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HYJ15574.1"
                     /translation="MANSRTHRALIQRLVLTLFLLLRLAPFVAAEDLPVTEKQKIETL
                     INQVKSLKDAKFVRNGRSYSADNAATFLKGKWDANAADMTSAQDFVDRIASFSGTSGK
                     PYLIRFQDGKEIKC"
BASE COUNT          918 a         1176 c          983 g          725 t
ORIGIN      
        1 tgaccaaagt cctggggctc gatctgctag aacggcgcga tcgtgaagcg gtcttgaagc
       61 atcccaacac cggctggaaa ttaatcgtcc atgaaggcgg cgccgaggtg aaagacaaac
      121 ccgagcgcaa tcactacggc gtgcgcgtag ccaacaacct agaagtcgat cacgcctacc
      181 agtatctcct cgccaacaaa gaacgcttga gcctcactaa agtcgtcaaa cggaaagaac
      241 gcgccggctc ctactcgatg tttttcgtcg aaccaggcgg caattactgg gagatcgagt
      301 cttacgaaaa tcgccacaag gcgggtctac cctatgaagt cgcctatccg tggaagaccg
      361 cgctaagcga ggaacgcttg ccaggctgcg gttacgtgcc ccaggcgatg actcatggca
      421 ccatggaatg caccgatctc caaaggtcaa agaagtttta tcagcagggc ctaggactcg
      481 atgtcatcac ccatgttccg accatcgagc cccatgacat caaacatcca accacgccct
      541 ggtacgtcgt gagcttggaa gtgccgccaa agaacaagca ctatttgacg ccgctgcaac
      601 gttacaccgt cgccgttgag tcatccgccg ctctcaccga agcgaatcgg caattcaccg
      661 agcgccggga tgagttcggc ctctgcgcca tcgaagccgt tcaggacagc cgcgccggcc
      721 agtccttcca gatctgtgac ttggatcgca actggtggga ggttgcctac atcgcgaact
      781 agcaccatgg aactctcgaa tcctagcttg atccgaccga tcggtccgat ccgactgatc
      841 ttcgcagccg ctctcctgtt aacgctcacg gctctcgccg ccgctcagcc aataaaactc
      901 aatgtcgcct atacctcgac gacaaccaac ttctcaatcg cctgggtcgc caagctcgaa
      961 ggcatctacc gcaaacataa tctcgacgtc gaattgatct tgatgcaggg gccatcgact
     1021 tatttgccgg cactgttgag cggcaatatt cacgtccttt acggcggcgg cacggcggtg
     1081 tcgcgcgcca tcgccgccgc cgatcccaac atcatcgtca tcggcagcga gacccgctac
     1141 gtgccgttgc gcctgatggt caccccgtcg atcaaaagcg ccgcggattt gaaaggcaaa
     1201 agactgggtg tcggccgcgc cggtcttgat gaatacgcaa cgctctatta cctggaaaaa
     1261 aacaatctgg ttcccaataa agatgtcgcg ctgatctaca cgaccggcgg tatccttgcg
     1321 cgtgccgcgc agatgaagca ggggctctat gacggcatgg cgattccgcc gcccaacgag
     1381 atcgagcttg agaagcaggg ctttcgcgag ctcgcttact ttttcgatct gcgcatgtcc
     1441 tacgccggca tcccgtatac ggtgacacgc gattttcgcg acaagaatca gcgcgtgctg
     1501 accgatttca tgaccgctat taccgaagcg atccaacttt atcgcatgaa taaagaagtc
     1561 ggctaccgct ccatcgtcca aatcacccgc caaaacgacc cgatggtttt ggaaaagacc
     1621 tatcgcgcca atctgcagca gtacgatgcc atccagggct tgccattccc gtggcaggac
     1681 ggtatcgaaa gtatgatcaa cggctttcac gcccgcttca cgccggcgct catcaagaac
     1741 cgcgacgctc gcccctacct cgacccatcg ttcgtccaac gagcagtcga acgtttgggg
     1801 ctggtcaaga aatagctgcg gagaactcca tgtcagacac agcgaaaccg gcagttgcat
     1861 ccctgggcat caccaagggc cattacgagt gcaactacct tgaccagacg gtgccggtgt
     1921 tgtcggaatt gcttgccctg gaagtcgtgg caaagggcga tcgagaagtt accatgaaac
     1981 atcccaatac cgactggctg ctggtcgtgc acgaaagcgg ccccgccgtg ccggacaaac
     2041 cgtttcgcaa ccactacggc gtgcgcgtta agaacaacga gcaggtcgac cgggcctacg
     2101 aatacttgat ggtgaaaaaa gaacagctag cattgaaaaa agtcgtcatg cgcaacgagc
     2161 gcgccggttc ctactcggtg tttttcgtcg agcccggcgg caactactgg gagatcgagt
     2221 cctacgagca ccgccaccaa gcaggcctgc cgtacgaggt ctcctatccg tggcagaccg
     2281 aactcagcga agcacagttc cccggccgag gttacctgcc gcacggcttc acccacggca
     2341 ccatcgaatg caacgactgg gcttcgtcgg taaaattcta caaagacggc ctgggcatgg
     2401 acgtcatcac ccatgtccaa accaccaaac cgcacaacat caaacacccg gcgaattctt
     2461 ggtacgtggt cagcctcgaa gtgccggaga aaagccgcaa atatctatcg ccgctgcagc
     2521 gcttcaccat cgccgtcgcc tccgtggatg agctccggga agcgcgcgaa aacttgagcg
     2581 cgcggcgaaa cgaattcgcg atcacggaga tcggcgatat tcaagatacc ctggatggca
     2641 aggctttcat gctttgcgac ttgaacaaaa actggtggga gatcgccgct ccgctccgtc
     2701 attcccgtga cagcgggaat ccaggctaat tcaaattcac tggaggtcta aaacgatgaa
     2761 tgtgcaccga atcgacaaca aaaaaaccgc cctgctgttc ttcgacatgc tcaacgtcta
     2821 cttccacggc atcccggatg aaaagcataa agccttgaag ccggtggtcg ccaacgccgt
     2881 gcgtttgatg aacgccgcgc ggcaaaatgg cattctgatc tactacgcca tggccaatca
     2941 tcgacccgac ggcgaaagcc gctatcgggc gatcaccgac accgacatgc gcttgcgccc
     3001 ctggcccaac gacgattgca atcccaccgt gcacggcgcc accgaaggct cgcgggaaca
     3061 aaaggtcctc gatgaaatcg cgccacagcc gcaggattac attattccga aataccgttg
     3121 gagcaccttt catcagactt acttcgactt ggcgctgcgc tcccgcggca tcgacacgat
     3181 catcatctcc ggcggcgccg tcgacgtcgg cgtcgcctcg acggtctact ccgcccgcga
     3241 ccacgactac aatctcgtca tcgtccgcga cgcgtgcagc aactcccacg acgactccat
     3301 ggccgcgctt atgaacacgg tttttccccg gctggcccgg gtccgcagca ccgaccaggt
     3361 gctcgatatg attcggaccg ggtaatatta tttccaggcc gacgattgag agccgaaggc
     3421 aaaggtttgg agccagtcaa cgtctaagtg acaaatggct aattcgcgaa cccacagagc
     3481 tttaatccag cggctcgtct taacgctttt tttgctcctg cggttggcgc cgtttgttgc
     3541 ggccgaagac ctccccgtca cggaaaagca aaagatcgaa acgctgatca accaggtcaa
     3601 gtcactcaaa gacgccaaat ttgtgcgcaa tggccggagt tacagcgccg acaacgccgc
     3661 gacgttttta aaaggcaaat gggacgccaa cgcggcggac atgacaagcg cccaagactt
     3721 cgtcgacagg atcgccagct tttccggcac atccggcaag ccttacttga tccgttttca
     3781 ggacggcaaa gaaatcaaat gc
//
LOCUS       DASQKS010000338         5570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3959433, whole genome shotgun sequence.
ACCESSION   DASQKS010000338 DASQKS010000000
VERSION     DASQKS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3959433"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VE170_08800"
     CDS             <1..640
                     /locus_tag="VE170_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018649476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ15575.1"
                     /translation="GKSTLLKHMIGLYAPAAGTILIDGDNVATVEGQERQQLLRKFGV
                     MYQSGALFGSMTVLDNVCLPLQEFTDLPPDAIRMIASLKLQRVGLGGFDNHMPSELSG
                     GMQKRAAIARAMALDPQVLFLDEPSAGLDPITSVELDDLILSLARSLKITFVIVSHEL
                     ASIFAIADRVIMLDKSVKGIVATGKPEELRDRSDNPWVRQFFSRQPSADKPV"
     gene            680..1729
                     /locus_tag="VE170_08805"
     CDS             680..1729
                     /locus_tag="VE170_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYJ15576.1"
                     /translation="MEKASYFKIGLFIIVGTVLGAIGVVALGVGTIFQKKVMVETYID
                     ESVQGLDVGSSVKFRGVQVGKVETISLTSAEYPTKRRYVLVRIGLTTKIFLADAGSPS
                     FLAEVEKGLRVRLASQGVTGAAYIEADYQDPARNPPLEIDWQPRYPYVPSSRSRITQL
                     SESVEKILRSVEDIDVGRLVDGLEKSLLTITKVAEGANFDKLGGQANAFLTEVRETNR
                     QLKELIGNSDIKSAFKDSAAAASGARQIIERAQAPVNQMLADLPRAAESLERMVKRLD
                     AVTADLPETSAQLRQTLQRMNRLIATQQQGIAATVENLQAVSSDIKELTESSRKYPSQ
                     VLFGAPPPPSKVMQR"
     gene            1726..2346
                     /locus_tag="VE170_08810"
     CDS             1726..2346
                     /locus_tag="VE170_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15577.1"
                     /translation="MKLCRTHYVAAALLAMLGGCVSLERLERSYPEKRFYVLEVREPA
                     PAGGAVQDAILQVSTLRISPRYQDRSFVYRISDNGFEADFYNQFIAPPAGLISEEIRK
                     SLIGAELFKYVISASNSQAPSYVLEGAVNGIYGDFRNRNAPAAVIDLEFFLTSDLPAK
                     PGIVLQKRYVKSVPVSSRSPEALVKSWDLALEEILTALIADLKAVR"
     gene            complement(2443..4851)
                     /locus_tag="VE170_08815"
     CDS             complement(2443..4851)
                     /locus_tag="VE170_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYJ15578.1"
                     /translation="MMSKSAKTVIEVKEVRGYCALCTAHCATIATVENGRVTKLEPDH
                     DHPNGGVMCVKGKAAPELVYNSDRLNYPLKRTRPKTDADPGWQRVSWDEALDDIARRL
                     LSIREQHGAKAMALGKGTKSGTSVDDVERWLGRFLYLYGSPNWVSTTHVCNWHKDTGF
                     SFTFGTTIQTPDVAHSKTFLLWGHNPSSTSLILAHDIVAARQRGMKTVVVDPRRIGIG
                     ANADILLQVRPGTDGALALALIHCLIEESWYDVEFVRRWTNGMFLLNMATGAVVTEAD
                     LNAAGSRNRFVIWDEAKNETVIYDPHTGAYARDNVSPALFGRRVVRSQDGSEIICQPV
                     FERLGEIAAAFAPEKSAKITWVAADQVWQTALMLAHNRPLSMYMWNGVGQHTNATQTS
                     RAIATLYALLGDFDRQGGNVTFPKLPTNNVDGKEFLAKEAAALRIGREKKPLGPPASP
                     GNCAAYDIFTAILDEQPYPIKAFLAFGCNPVMSNPDPRRAREALSKLDFAVALDLFMT
                     PTAALCDYVLPATSFLEMSAITTAFEHRQSGKTHLQYRASVIEPLHERRSDTWILFEL
                     AKRMGMSEQFFNGDIEAAYAYELAPTGVTLEQLKNAPGGISRPLAPRFEKHATVKKDG
                     TPRGFDTSSKRVELYCHKFAAAGYPALPEYLEPAMSPVSCPEIAADFPLVLTNAKNTS
                     FVHSQHRSLTSLRKSLPEPSADVHPETALRYGVKHKEWMTVETPRGAIKARARVTTNI
                     LPGAVCVQHGWWQGCKELELPGYDAFEPTGSNAAMLIGTEHADPVSGSLPHRSYLCRV
                     RPAP"
     gene            complement(4968..>5570)
                     /locus_tag="VE170_08820"
     CDS             complement(4968..>5570)
                     /locus_tag="VE170_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ15579.1"
                     /translation="LLAQTPPHAWAIADTPMLAKIDVADPYYRRGDVIDRAKVEELLH
                     NSPFIKKADSLEELGKQCRFEDLKNFLGEVEAYNQCFDAKLDTDLKFGKSLKGSKKFD
                     MPPYYAIQICPLARKNFGGAKTDLRCRALNKYFEPIPGLYAAGEVAGMAGGHINGKAG
                     LEGTMLGPSIFSGRIAGAWAAHEAGFGPGFVGKPNRPELD"
BASE COUNT         1092 a         1554 c         1720 g         1204 t
ORIGIN      
        1 cggcaaaagc accctactca aacatatgat cggtttgtat gcgccggcgg ccggcacgat
       61 cctcatcgac ggcgacaatg tcgccacggt ggaaggccaa gagcgccaac aactattacg
      121 aaaattcggg gtcatgtatc agagcggcgc gctgttcggc tcgatgacgg ttttggacaa
      181 tgtctgtctg ccgctgcagg aatttaccga tttaccgccc gatgcgattc gcatgatcgc
      241 ctcgctgaag ctgcaacgcg tcggacttgg cggcttcgac aatcacatgc cgtcggaatt
      301 gagcggcggc atgcagaagc gcgccgccat cgcccgcgcc atggcgctcg acccgcaggt
      361 gttgtttctc gacgagccgt cggccggact cgatccgatc acctcggtgg agctcgacga
      421 tttgatcttg tcgctggcgc gcagcttgaa aataactttt gtcatcgtca gccatgagtt
      481 ggcgagtatc ttcgcgattg cggaccgggt gattatgttg gataaaagtg tcaaaggcat
      541 tgtcgccacg gggaaacccg aggagttgcg cgatcgcagc gacaatccct gggtgcggca
      601 gtttttttcc cggcagccgt ccgccgataa gccagtgtaa catcggagag cacaatctta
      661 gccgtctaat acaccgggaa tggaaaaagc cagctacttc aagatcggtc tgtttatcat
      721 cgtcggcacg gtcctcggcg cgatcggcgt ggtcgccctc ggcgtcggca cgatttttca
      781 aaaaaaagtc atggtcgaga cttacatcga cgaatcggtg caggggctcg atgtcggctc
      841 gtcggtgaaa ttccgcggcg tgcaagtcgg caaggtcgag acaatcagcc tgaccagcgc
      901 cgagtacccg accaagcgcc gctacgtttt ggtgcgcatc ggcctgacga cgaaaatatt
      961 tctcgccgat gccggctcgc caagtttcct ggctgaagtc gaaaaaggtt tgcgcgtccg
     1021 actggcctcc caaggcgtca ccggggcggc ttatatcgag gcggattatc aggatccggc
     1081 acgcaatccc ccactggaga ttgattggca gccccgttat ccctatgtgc cgtcgagccg
     1141 cagccgtatt acccagctga gcgaatcggt ggaaaaaatt ctccgcagcg tggaagacat
     1201 cgacgtgggg cggttagtcg acgggttgga aaagtcgctg ctgacgatca ccaaagtcgc
     1261 cgagggggcc aacttcgata aactcggcgg ccaggccaat gcctttttga ccgaggtgcg
     1321 cgagaccaat cgccagttga aagagttgat cggcaacagc gatatcaaat ccgccttcaa
     1381 agacagcgca gcggcggcga gcggcgcgcg ccagatcatc gagcgcgccc aagcgccggt
     1441 caaccagatg ctcgccgact tgccgcgcgc cgctgagagt ttggagcgga tggtcaaacg
     1501 gctcgatgcg gtcaccgccg atctgccgga aaccagcgcc caactccggc aaaccttaca
     1561 gcgcatgaac cgattgatcg cgacccagca gcaggggatc gctgccacgg tcgagaactt
     1621 gcaagcagtt tctagtgaca tcaaagaact cacggaaagt tcgcgcaagt atccatccca
     1681 ggtgttgttc ggcgcgccgc cgccgccctc gaaggtgatg caacgatgaa attatgtagg
     1741 actcattatg tggccgcagc gctattggcc atgctgggag gttgcgtcag ccttgaacgc
     1801 cttgaacgca gctatcccga gaagcgcttc tatgtcttgg aagtgcgcga gccggcgccg
     1861 gccggcgggg ccgttcagga tgcgattttg caagtgtcga ctttgcgtat ttcgccgcgc
     1921 taccaggatc gaagttttgt ttatcggatt tcggacaacg gttttgaggc tgatttttac
     1981 aatcaattta tcgccccgcc ggcgggattg attagcgaag agattcgcaa gagtttgatc
     2041 ggtgcagagc ttttcaagta tgtcatcagc gcgtcgaaca gccaggcgcc gagctatgtg
     2101 ttggaaggcg ccgtcaacgg gatctacggc gatttcagaa atcgcaatgc gccggccgcg
     2161 gtcatcgacc tggaattttt cctgaccagc gatttacccg ctaagccagg cattgttcta
     2221 cagaaacgtt atgtgaaatc cgtgccggtg agcagccgat cgccggaagc gctggtcaaa
     2281 agctgggatc tggcgctgga agaaatcctc actgctctca ttgcggatct taaagccgtt
     2341 agataatagt cgttccccta gttcaataca ttcaagccgt tagaacaatc ttcgattttg
     2401 ctcggctgct taaaaacgcc tggaatagag tcgcacggcg acttatggcg ccgggcggac
     2461 tctgcataga tacgaccgat gcggcagcga gccgctgact ggatcggcgt gttcggtgcc
     2521 gatgagcatg gcggcgttcg agccagtggg ctcgaaggcg tcgtagccgg gcaattctaa
     2581 ttctttacat ccttgccacc agccatgttg gacgcagacg gccccgggaa ggatgtttgt
     2641 cgtcacgcgg gctctggctt tgatcgcgcc gcgcggggtt tcgacggtca tccattcttt
     2701 atgcttgacg ccataacgca acgcggtttc cgggtgaaca tcggcgctcg gttcaggcag
     2761 cgatttgcgc agactcgtca gggagcgatg ttggctgtgg acgaagctgg tgtttttcgc
     2821 gttggtcagg accagcggaa aatcggccgc gatttcggga cagctgaccg ggctcatcgc
     2881 cggctcgagg tattccggca gcgctggata gccggcggcg gcgaacttgt ggcaatagag
     2941 ttcaactcgt ttcgatgacg tgtcgaaacc gcgtggggtt ccatcttttt taaccgttgc
     3001 gtgcttctcg aagcggggtg ccagcggacg ggaaatgccg ccgggagcgt tcttcaattg
     3061 ttccagcgtg actcctgtcg gtgcgagctc atacgcatag gcggcttcga tgtcgccgtt
     3121 gaagaattgc tcgctcatgc ccatgcgctt cgccagttcg aagagtatcc aggtgtccga
     3181 acggcgctcg tgtagcggtt caatcacgga ggcgcggtat tgcagatggg tcttgcccga
     3241 ctggcggtgt tcgaacgccg tggtaatggc ggacatttcg agaaaactgg tggccggcaa
     3301 aacataatcg catagcgcgg ccgtcggcgt catgaacaaa tccagcgcca cggcgaaatc
     3361 caatttgctc agcgcttcgc gggcgcggcg cgggtcgggg ttggacatca ccggattaca
     3421 gccgaaggcg agaaacgctt tgatcgggta gggctgttcg tcgaggatcg cggtgaagat
     3481 atcataggcg gcgcagttgc ccggcgatgc cggcggaccg aggggctttt tctcgcggcc
     3541 gatacgcagc gccgccgctt ctttcgctag gaactctttg ccatcgacat tgttggtcgg
     3601 cagcttggga aatgtcacgt tgccgccctg gcggtcgaaa tcgccgagca gcgcgtagag
     3661 ggtcgcgatg gcgcggctgg tttgggtcgc attggtgtgc tggccgacgc cgttccacat
     3721 gtacatgctg aggggccggt tgtgtgccag catgagcgcg gtctgccaaa cttggtcggc
     3781 cgcaacccag gtgatttttg cagatttctc cggcgcgaat gcggcggcga tctcgccgag
     3841 ccgttcgaag accggctgac aaataatttc gctgccgtct tgcgagcgca caactcgcct
     3901 gccgaagagt gcggggctca cgttgtcgcg ggcgtacgcg cctgtgtgag gatcgtaaat
     3961 gacagtttca tttttcgcct cgtcccagat cacgaaacga ttgcggcttc ctgctgcgtt
     4021 gagatcggct tcggtgacga ccgcgccggt tgccatattc agcaggaaca tgccgttggt
     4081 ccagcggcgc acgaactcga cgtcgtacca actctcctcg atcaagcaat gaatcaacgc
     4141 cagcgccagg gcgccgtcgg tgccggggcg aacttgcagc aatatgtcgg cgttggcgcc
     4201 gatgccgatg cggcgcggat cgacgaccac ggttttcatg ccgcgttggc gggctgcgac
     4261 gatatcgtgg gcgaggatca acgatgtcga gctcgggtta tggccccaga gtaaaaaagt
     4321 cttgctatgc gcgacatccg gcgtctggat cgtcgtgccg aaggtaaaac tgaagccggt
     4381 gtctttgtgc cagttgcaaa catgagtggt cgaaacccag ttcggactgc cgtagagata
     4441 gagaaaccgg ccaagccagc gctcgacatc gtccaccgag gtgccgctct tcgttccctt
     4501 gcctagcgcc atcgccttgg ccccatgctg ctcgcggatc gacagcaatc ttcttgcgat
     4561 gtcgtcgagg gcttcgtccc aactgacccg ctgccacccc ggatcggcgt cggtcttggg
     4621 ccgggtgcgc ttgagcggat aattcaagcg gtcggaatta taaaccagct ccggcgcggc
     4681 cttgcctttg acgcacatga cgccgccgtt gggatgatcg tggtccggct ccaactttgt
     4741 cacccgcccg ttctcgacgg tggcgatggt ggcgcagtgg gccgtgcaaa gggcgcagta
     4801 gccgcgcact tctttaactt cgattacggt ctttgctgat ttggacatca tgatccccgt
     4861 ctgcgacttg cgcgctgtgc gcggctgttc aaggttcaag ggttcaaagg ttcaaggccg
     4921 gattccggac gattgaactt ttgaaccttg aacgtttgaa cggcattcta atccaattcc
     4981 ggccggttcg gtttgccaac aaatccgggt ccgaagccgg cttcgtgggc ggcccaggcg
     5041 ccggcgatgc ggccgctgaa gatcgagggg ccgagcatgg tgccttcgag gccggctttg
     5101 ccgttaatgt ggccgccggc catgccggcg acttcgccgg cggcgtagag gcccgggatc
     5161 ggctcgaaat atttattcag cgcgcggcag cgtaggtccg tcttcgcgcc accgaaattt
     5221 ttccgcgcca gcgggcaaat ctgaatcgca tagtagggcg gcatgtcgaa cttcttcgaa
     5281 cccttgagcg acttgccgaa cttgaggtcc gtgtccaatt ttgcatcgaa gcattgattg
     5341 taggcttcga cctcgccaag aaaattcttc aggtcttcga agcgacactg cttgcccaac
     5401 tcttcgaggc tgtcggcctt cttgatgaac ggcgaattgt ggagcaactc ttccaccttc
     5461 gctcgatcga tgacatcgcc gcggcgatag taggggtcgg cgacgtcgat ttttgccagc
     5521 atcggcgtat cggcgatggc ccaggcgtgg ggcggcgttt gcgcgagcag
//
LOCUS       DASQKS010000339         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1850896, whole genome shotgun sequence.
ACCESSION   DASQKS010000339 DASQKS010000000
VERSION     DASQKS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850896"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VE170_08825"
     CDS             complement(<1..554)
                     /locus_tag="VE170_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15580.1"
                     /translation="MGKFHVVYFGSNDVPVDLLKPILAEIDADMTIRRPTTDAEVIEM
                     AKDADGIIMHGSVPLSREIIAQLKRVKVVCRTGVGVDRMDLKAAADHGLIICNAAGCN
                     SIEVAEQAIGLMIAISRKLVRMNQYVHDGKWKRHTAELHAYRGRVYRIQGCTMGIVGL
                     GHVGKQVAPRAQGMRLNVQTFDPYL"
     gene            complement(560..1693)
                     /locus_tag="VE170_08830"
     CDS             complement(560..1693)
                     /locus_tag="VE170_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15581.1"
                     /translation="MRDGFKIIDADRHVLEPSDLFAKYLPEKFRSRVDIQGPNQSYRR
                     VDGELISDADRLRGATKQEDFGFTFAASKKWRETFADALAAKFDTASNVRDMDKEGVD
                     IGVLFPTVGLYIMWRDNLDPELSAAICRAYNTWMSDYCSASDKRLYGVALIPLQDPVR
                     AVEELEHAKKLGLVGIFWRPNKLCGRTIASPDYDPIYAAAADLGMTVCVHEGARTVLA
                     QAGSDRYSEFGRHVACHPLEQMLACLNLCADGVLEKFPKLKVAHLESGCGWVPFWLER
                     MDEHWEHAGHGQAKTTKEKPSFYFKRQCWTSAEAGEELAPVFVEHVGEDYLLVATDYP
                     HSDCIGKFPDRTVGDLTTNNHLSREARRKILWDNPKRFYGLEV"
     gene            1910..3598
                     /locus_tag="VE170_08835"
     CDS             1910..3598
                     /locus_tag="VE170_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ15582.1"
                     /translation="MNVRRTRLVTGLILLTYLSTHLLNHALGLISLAAMEWGRRWFLL
                     LWRNPFGTAMLYSALLTHLCLAFWALYQRRHWRMPLSEMLQLSFGLTIPLLLTIHIVG
                     TRLTHEFFGVDDSYTLVVLTLWHAAPMDGLKQAILIIIAWTHGCIGCHYWLRLKKGYP
                     RLAPYLLTLALLIPVCGLLGFVAAGREVANLIAHQPDWLENFLVAAKVPPRSQRGVLY
                     QIYDGVLITFGAALALALLARVVRNLVERRSRIRITYPNGREVQAPRNFSVLEASRLA
                     AIPHASVCGGRGRCSTCRVRVTLGAANLPPPTAGELKVLQRVGAAPDVRLACQLRPTN
                     NLTVTPLLPAHAQASDGYGQPAYLAGQERMIAVMFADIRSFTGIAETKLPYDLVFFLN
                     SYFAAVGIAIASNGGMIDKFVGDGVMALFGVESGPEEGCRQALAAARAMIERIDGLSG
                     AFAEELSAPLRIGIGIHCGPAVVGRMGYGESIHVTAIGDTVNVASRLQDATKEFHCQL
                     VLSEEVAKQSGLNTAGLPRHELTVRNRREALTIYALDDVSKIAERSRLVSGDRL"
BASE COUNT          703 a         1042 c         1162 g          826 t
ORIGIN      
        1 agatagggat cgaaggtttg cacgttcaag cgcatgcctt gggcgcgtgg ggcgacttgt
       61 ttgccgacat ggcccaagcc gacgatgccc atggtgcagc cctggattcg atacacgcgg
      121 ccgcggtagg cgtgtagttc agcggtatgg cgcttccatt tgccgtcgtg gacgtattgg
      181 ttcatgcgca cgagtttgcg cgagatcgcg atcatcaggc cgatcgcttg ctcggcgact
      241 tcgatggaat tgcaaccagc cgcgttgcag atgatcaggc cgtgatcggc ggcggctttg
      301 aggtccattc tatcgacgcc gacaccggtg cggcagacga ctttgacccg ctttaactgc
      361 gcaatgatct cgcgcgatag cggcaccgaa ccgtgcatga tgatgccgtc ggcgtccttg
      421 gccatttcga tcacttccgc gtcggtggtc gggcggcgga tggtcatgtc ggcgtcgatc
      481 tcagccagta tcggcttgag cagatcgacc ggcacatcgt tgctgccaaa ataaactacg
      541 tgaaacttac ccatttgagt caaacctcca gtccataaaa gcgcttcggg ttgtcccata
      601 aaattttccg gcgcgcttct cgtgagagat gattgttggt ggtgagatcg ccgacggtgc
      661 ggtcggggaa cttgccgatg cagtcgctgt gtggatagtc ggtggcgacc agtaagtaat
      721 cttcgccgac atgctcgaca aaaaccggcg ccaactcttc gccggcttcg gcgctggtcc
      781 agcattggcg cttaaaatag aagctcggtt tttccttggt cgttttggct tggccgtggc
      841 cggcatgttc ccagtgctcg tccatgcgtt ccaaccagaa gggcacccag ccgcatcccg
      901 attccaggtg agcgaccttg agcttgggaa atttctcgag cacgccgtcg gcgcagagat
      961 tcaggcaggc gagcatctgc tccaagggat ggcaggcgac atgtcggcca aactcgctgt
     1021 aacggtccga gccggcttgc gccagcacgg tgcgcgcgcc ttcgtgcacg cagacggtca
     1081 tgccgaggtc ggcggcggcc gcatagatcg gatcgtagtc ggggctcgct atggtgcggc
     1141 cgcaaagctt gttaggccgc cagaagatgc cgaccaggcc gagctttttg gcgtgctcta
     1201 gctcctccac cgcgcggacc gggtcttgaa gcggtatcaa ggcgacgccg tagagtcgct
     1261 tgtcgctggc gctgcaatag tccgacatcc aagtgttgta ggcgcggcag atggcggcag
     1321 acagttcggg atccaaattg tcgcgccaca tgatgtacag gccgacggtg gggaagagga
     1381 cgccgatgtc gacgccttct ttgtccatgt cgcggacgtt ggaggcggtg tcgaacttgg
     1441 ccgccaaggc gtcagcgaaa gtctcgcgcc attttttcga cgcggcgaag gtaaagccga
     1501 agtcttcttg tttagtcgcg ccgcgcaacc ggtcggcgtc ggaaattaac tcgccgtcta
     1561 ctctacgata ggactggttg ggtccctgga tgtcgacccg gctgcggaat ttttccggta
     1621 aatatttggc gaacagatcg gacggctcga gcacgtggcg gtcggcgtcg atgatcttga
     1681 atccatctct catgacgaag ctccttctcg actgctattt atattcttag cagtaggaca
     1741 atgaggcagg gtgagtcaac agcagagggg cgaaaatggt cgacgcaatt tcgccatcaa
     1801 gaagtcatct tacgagattc gcctttggca cggactcgcc ggtttatccg cctccgatgc
     1861 aacaacatag acgttaccgg cgcactcgta gtagattggc gcccaagcta tgaacgttcg
     1921 gcgcacacgt ttggttaccg ggctaatctt gctgacctat ttgtcgacgc acttgctcaa
     1981 tcatgcgctc ggcttgattt cgctggcggc gatggagtgg gggcggcggt ggtttttgct
     2041 gttgtggcgc aatccgttcg ggacggcgat gctttacagc gcgttattga ctcacctatg
     2101 tctggcgttc tgggctttgt accagcggcg ccattggcgc atgccactct ccgagatgct
     2161 gcaactttca ttcggtctga ccattccgct gttgctgacg atccatatcg tcggcacgcg
     2221 actcacccat gaattctttg gcgttgacga ttcctatact ttggtggtct tgaccctgtg
     2281 gcatgctgcg ccgatggatg gactgaagca ggcgatactg attatcatcg cctggaccca
     2341 tggctgtatc ggttgccatt actggctgcg attgaagaaa gggtatccgc gtctcgcgcc
     2401 atacctgctc actttagcgt tgcttatccc ggtgtgcggg ttgctcggtt tcgtcgcagc
     2461 cggcagggag gttgccaatc tgatcgcgca ccagccggac tggctcgaga acttcctcgt
     2521 ggctgcgaaa gtgccgccgc gcagccagcg cggggtgttg taccagatct atgacggcgt
     2581 gctgattacg ttcggggcag cgctcgcctt ggcgctgttg gcgcgggttg tgcgaaattt
     2641 ggttgagcgg cgcagccgta ttcgcattac gtatcccaac ggccgggagg tgcaggcgcc
     2701 gcgcaatttt tccgtcctcg aagcgagccg tttggcggcg atcccccatg cttcggtttg
     2761 cggcgggcgc gggcgctgct cgacctgccg ggtgcgggtc actcttggcg cagcgaattt
     2821 gccgccgccg actgccggcg agttgaaagt cttgcagcgc gtcggcgccg cgcccgatgt
     2881 cagactcgcc tgccagctcc ggccgacgaa taatcttacg gtgaccccgc tccttccggc
     2941 ccatgctcag gccagcgacg gttatgggca gccggcttat ctcgccggcc aggagcggat
     3001 gatcgccgtg atgttcgcgg atatccgctc gttcaccggc atcgctgaaa ccaaactgcc
     3061 gtacgactta gtgtttttct tgaacagcta tttcgcagcc gtcggaatag cgatcgcgtc
     3121 caacggcggc atgatcgaca aatttgtcgg cgatggcgtg atggccctgt tcggcgtcga
     3181 aagcggaccc gaagaaggct gccgccaagc gctcgcggcc gcccgagcga tgatcgagcg
     3241 gatcgacgga ctcagcggag cgttcgcaga agaacttagc gcgccactgc gcatcggcat
     3301 cggcattcat tgcggcccgg cggtggtggg acgcatgggc tacggcgagt cgattcacgt
     3361 taccgctatc ggtgatacgg tcaatgtcgc cagccgtctg caagacgcga ccaaagaatt
     3421 ccattgtcag ttggtgcttt cggaagaagt cgccaagcag tcgggattga acactgccgg
     3481 cctgccgcgc cacgaactca cggtgcgcaa ccggcgcgag gcgttaacga tttatgcgct
     3541 ggacgatgtt tcgaagattg ctgaacgatc gcgcttggtc agcggtgatc gtctgtaaga
     3601 tggttttcac cacgaaggct ccagcgcggc gcagcgacaa ccgaaaagaa gaatggccgc
     3661 aaagaacgca aagggcgcgc aaaaaacatc ggatagtagt acgaagacga agttaagaaa
     3721 taatagattc cga
//
LOCUS       DASQKS010000340         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3545658, whole genome shotgun sequence.
ACCESSION   DASQKS010000340 DASQKS010000000
VERSION     DASQKS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545658"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..1956
                     /gene="trpE"
                     /locus_tag="VE170_08840"
     CDS             469..1956
                     /gene="trpE"
                     /locus_tag="VE170_08840"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYJ15583.1"
                     /translation="MIQPSFKEFCRLAKFGNLVPVYQELLMDLETPLSFFKRLERDRY
                     AFILESVEGSERWARYSFLGTRPQRIFKARGNQVEVIDNGKVRKWQTAEPLKALQELL
                     KDYRPVPVAGVPPFFGGALGYVAYDAVEQFHGIVNRKKDPLGMPEIFLLFVQTLIAFD
                     NLKHTIKVIDNIHIEPGTNLRRAYDSAIKRIHQVISTLQKKPRGIEPRDLSPASGKRK
                     FRSNLSRQAFYKAVDRAKEYIKAGDIIQTVLCQRLESETDSDPFEIYRALRFVNPSPY
                     MYYLELDDLRVIGSSPETLVRLTGDTIELRPIAGTRRRGASADEERELEAELLADPKE
                     RAEHIMLVDLGRNDVGRVAAIGSVEVNELMAIERYSHVIHIVSNVRGKLAQDKTPFDL
                     FVSAFPAGTLSGAPKIRAMQIIAELEPQKRSLYGGAIGYFGYNGNLDTCIVIRTIVMK
                     GKKLFINAGAGIVADSDPALEYQETLNKARAMLRAVELAEQWSKR"
     gene            1953..2516
                     /locus_tag="VE170_08845"
     CDS             1953..2516
                     /locus_tag="VE170_08845"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HYJ15584.1"
                     /translation="MILLIDNYDSFTYNLYHYLGELGAEVRVVYNDKISLDEITALRP
                     EKIVISPGPCTPKEAGISCAVVNRFGANTPILGVCLGHQSIGAAFGGKIVRAPSIMHG
                     KLSDVSHDAKTIFQGLKNPFAAMRYHSLVIDPQSLPAQLVVSAQTDDGVIMAVRHNTF
                     PVEGVQFHPESILAEAGKQLLKNFLDR"
     gene            2759..3178
                     /locus_tag="VE170_08850"
     CDS             2759..3178
                     /locus_tag="VE170_08850"
                     /inference="COORDINATES: protein motif:HMM:NF020489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HYJ15585.1"
                     /translation="MKLLCFQAKRFHWKSHSKTLAEVADQDIDQEVCQTVVVFLHAEA
                     ADSDAERSAAVLRQSLKHIKWLANKRELKNIVLHSFTHLGGVTAPAALAESFLTALQQ
                     RLSETGYQVWTTPFGYFCEWDLSVYGESLAKVWKEIH"
     gene            3251..3814
                     /locus_tag="VE170_08855"
     CDS             3251..3814
                     /locus_tag="VE170_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYJ15586.1"
                     /translation="MKSDSKKLHCWTVALAILLLVTACKKTEEVASPDDLPDLNKQVR
                     PYVTIENAKLRSGPGPQFRGIADIPANAKIHVVGRDGDWALIISKKGNAPGFIELAAI
                     KPGEGDSAIDKPEFGEARRVEGRYEAMADTQVRSGPGLHYSALADIAKGTKLNVVAEE
                     KGWLKVESKSGNKPGYVEASLATPVSE"
     gene            complement(3839..4807)
                     /locus_tag="VE170_08860"
     CDS             complement(3839..4807)
                     /locus_tag="VE170_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15587.1"
                     /translation="MVVDFQHHYIPVELAKKRGLYSDTGRTLLQEGGLPATTMHQRLY
                     DLELQLKDMEEAGVDVSVLSCLLGWSAPLDECRFINDDLASIEKKYPRHFVGLAQAPV
                     LEGKAAIAELRRALADLGLRGVTITSQVGGLSLDAPKLYDLYELACELDVPVFVHPAL
                     VPTGYEHLKDYDLPRVLGREVDLTVATTRLIAGGIYDRYPSLKIVMAHFGGGIAAVKD
                     RLVGKGYRFGTLKKPFGEYYDMVYFDMAGFEGGTTALNCALEGIRPERLVFASDYPQD
                     FTGVNTDTGKGMSELRNYVEAVRKLPLSAKNREDMLGGTATKLLKI"
     gene            complement(4828..>5479)
                     /locus_tag="VE170_08865"
     CDS             complement(4828..>5479)
                     /locus_tag="VE170_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15588.1"
                     /translation="LRTAKPLVDVKIFRNKMLSAAMIALILSHILHNPIALAAPLYLQ
                     NVLGASAIAAGFLLAILPLSTALAAPLSGRLADRFDAASVATIGIGLIVAGIASYACL
                     GVDAHFASVGAVLALLGAGIGFFTPANQKLAFAAVAKEDYGVLAAMLSSFGTAAGTIG
                     TTIAVALMEMSGGDEIWKSQAVFAAAQQFAFAWLVPIGLVAIVVALRARSRNGEII"
BASE COUNT         1285 a         1637 c         1459 g         1098 t
ORIGIN      
        1 agaccaaaaa tggcgtttta agctaaccca ccgagcttgt caaaccatgc gaacccgggc
       61 tctcagcgcc ctacgaactt attccacgcc tgataattgg tcagacaatc gccaacgata
      121 aacaaccgat gtcacaaatc gacccctgac gaagcggact tcgggccgga accgctgatg
      181 gcatctgcac cgaagtcaaa tccttttatc aaatgctacc gaccgccgga ttaggcgacg
      241 cgaatccagc cgttcgcgtg cgcttaatcg ccatccttca gcgcacttga cgcctcaaga
      301 tctatacggc actatgacgc tcatgacgcc cattgagaat aatgtttggt ctggctggcg
      361 gcgctggtat tggaaccggc aacatcacct tggctgaata cgtctgtcga tctaactaaa
      421 aaaaagggcc ataggtgaat cacttatggc ccttttaatt tatccgctat gatccaacca
      481 agctttaaag aattctgccg gctcgccaag ttcggtaacc tagtgccggt ctatcaagaa
      541 ctcctgatgg atttggaaac gccgctgtcg tttttcaagc gtctcgagcg cgaccgctac
      601 gcattcattt tagaaagcgt cgaaggcagc gagcgctggg cgcgctattc gtttctcggc
      661 acacggccgc aacggatctt caaagcgcgt ggcaatcaag tcgaagtcat cgataacggc
      721 aaggtcagga aatggcaaac cgccgagccg ctaaaagcgt tgcaagaact gctaaaggat
      781 tatcggccgg tgccggtggc cggtgttccg ccctttttcg gcggcgcctt gggctacgtc
      841 gcttacgatg ccgtcgagca gtttcatggc atcgtcaacc gcaagaaaga cccgctcggc
      901 atgcctgaga tctttctatt gttcgtgcaa accctgattg ccttcgacaa tctgaaacac
      961 accattaagg ttatcgacaa catccacatc gaaccgggca ccaatttgcg ccgggcctac
     1021 gatagcgcga tcaagcgaat tcatcaggtc atctcgacgt tacagaaaaa accgcgcggc
     1081 atcgaaccgc gcgacttgag cccggcctcc ggcaagcgca aattccgctc caacttgagc
     1141 cgccaagctt tttacaaggc ggtcgaccgg gccaaggaat atatcaaagc cggcgacatc
     1201 attcagaccg tgttgtgcca aagacttgag agtgaaaccg acagcgatcc gtttgaaatc
     1261 taccgcgccc tgcgattcgt caatccgtcg ccttacatgt attacttgga gttggacgac
     1321 ctgcgggtca tcggctcgtc gccggaaacc ctggtgcgcc tcaccggcga taccatcgag
     1381 ctgcgcccca tcgccggcac gcgcaggcgc ggcgccagcg cagacgagga gcgcgagctc
     1441 gaagcagaat tactggcgga tccgaaagaa cgcgcggagc atatcatgct ggtcgatctc
     1501 ggccgcaacg atgtcggccg cgtcgccgcc atcggcagcg tcgaagtcaa cgagcttatg
     1561 gccatcgaac ggtattccca tgtgattcac atcgtctcca atgtgcgcgg caaactggcc
     1621 caggacaaaa ccccgttcga tctttttgtc tcggcgtttc ccgccggcac cctctccggc
     1681 gcgccgaaga tccgcgccat gcagattatc gccgaactgg aaccgcagaa gcgcagcctc
     1741 tacggcggcg ccatcggcta cttcggctat aatggcaatc ttgatacctg catcgtcatc
     1801 cgcaccatcg tcatgaaagg caagaaattg tttatcaacg ccggggccgg catcgtcgcc
     1861 gattccgatc cggcgcttga ataccaggag accttgaaca aagcccgcgc catgttgcgc
     1921 gccgtggagc tcgccgagca atggagcaag cgatgatcct gctcatcgat aactacgatt
     1981 ccttcaccta caatctgtat cactatctgg gcgaactcgg ggccgaggtg cgggtcgtct
     2041 acaacgacaa aatcagcctc gatgaaatca ccgcgctgcg ccctgagaaa atcgtcatct
     2101 cgccggggcc gtgcacaccg aaggaggccg gcatttcctg cgctgtggtc aaccgcttcg
     2161 gcgccaacac gccgatcctc ggcgtctgtc taggacatca gtccatcggc gccgccttcg
     2221 gcggcaagat cgtccgcgcg ccatcgatca tgcatggcaa actgtcggac gtaagccacg
     2281 atgccaagac gatttttcaa ggcttgaaaa atcccttcgc cgccatgcgc tatcattccc
     2341 tggttattga tccacagagc ttaccggcgc agcttgtcgt cagcgcccag actgacgacg
     2401 gcgtcataat ggccgtgcgc cataatacgt ttccggtcga aggcgtgcag tttcatccgg
     2461 aatcgatcct cgccgaagca ggtaaacaac tgctaaaaaa tttcctagac cgttaattcg
     2521 aaccgtagcc gttttaattc cgggtaaatt gttgctcgcc ccacgaaagc gacggccgat
     2581 caatcgatag gataattcgt tggtctcggc cagagacttg tgactgcgag atgaactcta
     2641 gcgctggtaa aattccgatc gtcgattcag cgattggctt ttcagtcgcg ctaactttgg
     2701 cgcaacgagt acccgcgaat tgcaacaacg ccgatcggtt gttaagttca ctcaatcgat
     2761 gaaactactc tgcttccaag ccaagcgctt tcactggaag agccattcaa aaactcttgc
     2821 cgaagtcgcc gaccaggata tcgaccagga ggtttgtcaa actgttgtgg tgtttctcca
     2881 cgccgaagcc gccgacagcg acgccgagag aagcgccgcg gtgctgcgtc agagcttgaa
     2941 gcacatcaaa tggctcgcca acaagcgcga gttgaaaaat atcgtgctcc attccttcac
     3001 ccatcttggc ggtgtcaccg ctcccgcggc cctagccgag tctttcttga ccgcattaca
     3061 gcaacgcttg agcgagaccg gctatcaagt ctggaccacg cccttcggct atttctgcga
     3121 gtgggatttg agcgtctacg gtgagagcct cgccaaagtt tggaaagaga ttcactaaag
     3181 ccgcttcgcc aaccgacgat ttgccatgtg ttatagtctc cagtagtaaa aatagctcgg
     3241 gaggaattcc atgaaatctg acagcaaaaa attgcattgc tggaccgtcg ctttggcgat
     3301 cctgttgctc gtgacagcct gtaaaaaaac tgaagaggtc gcgagccctg acgatctccc
     3361 cgacctgaac aaacaagtcc gcccgtacgt caccatcgaa aacgccaagc tgcgcagcgg
     3421 ccccggcccg caatttcgcg gcatcgccga catcccggcc aacgccaaga ttcatgtcgt
     3481 cggtcgcgat ggcgactggg ctttgatcat ttcgaagaag ggcaacgctc ctggattcat
     3541 cgaactggcg gcgatcaagc ccggcgaagg cgacagcgcg atcgacaagc cggaattcgg
     3601 cgaagcgcgc cgggtcgaag gtagatacga agccatggca gatacccaag tgcgtagcgg
     3661 tccgggactg cactattcgg cactggccga catcgccaag ggcaccaagc tcaacgtcgt
     3721 cgctgaagaa aaaggctggt tgaaggtcga atccaagagc ggcaacaaac ctggctatgt
     3781 cgaggcgagt ctggctacac cggtaagcga atagccgcgc cggcattgca cggcgacact
     3841 aaattttcaa taactttgtc gccgtgccac cgagcatatc ttctcggttc tttgcgctga
     3901 gcggcaactt gcgcaccgct tcgacatagt tgcgcagctc gctcatgcct tttccggtat
     3961 cggtgttaac accggtgaag tcctgaggat aatcgctggc gaaaactaga cgctcggggc
     4021 ggatgccttc gagggcgcag ttgagcgcgg tggtaccgcc ctcaaagccg gccatatcga
     4081 aatagaccat atcgtaatat tcgccgaagg gctttttgag cgtgccgaag cggtacccct
     4141 tgccgaccaa ccggtcctta accgctgcga taccgccacc gaaatgggcc atgacgattt
     4201 tcaagctggg ataacgatcg tatatgcctc cggcgatcaa gcgggtcgtc gccacggtca
     4261 aatcgacttc gcgccccaag acccgcggca gatcgtaatc ctttaaatgc tcgtaaccgg
     4321 tgggaaccaa ggccggatgg acgaacaccg gcacgtcaag ttcgcaggct aattcgtaca
     4381 aatcgtagag cttcggcgca tcaagagaca atccgcccac ctgggaggta atcgtaacgc
     4441 cgcgcaagcc caagtcggcg agcgcccggc gcagctcggc gatggcggct ttcccctcca
     4501 acaccggcgc ctgggccaaa ccgacgaagt ggcgtggata ttttttctcg attgacgcca
     4561 aatcgtcgtt gatgaagcgg cactcgtcga gcggcgccga ccatccgagc agacaagaca
     4621 acacggaaac gtccaccccc gcctcctcca tgtctttgag ctgcagttcc aagtcgtaaa
     4681 gccgctggtg catggtcgtc gccggaagtc cgccttcctg cagcaaagtt ctgcctgtgt
     4741 cggaatagag tccacgcttt ttcgccaact ccaccggaat atagtgatgt tgaaaatcga
     4801 caaccatgtc tgcttccttt cgttcgctca aatgatttcg ccgttgcgcg atcgagcacg
     4861 caatgccacg actatggcta ctagcccaat tggcaccaac caggcaaacg caaattgctg
     4921 cgcggcggca aacaccgctt gagatttcca tatctcgtcg ccgccggaca tctccatcag
     4981 tgccaccgct atagttgtgc cgatggtccc ggcggcggtg ccgaaggagc tcaacatcgc
     5041 cgccaacacg ccgtagtctt ccttggcgac cgcagcgaag gcgagcttct gattggccgg
     5101 ggtaaaaaaa ccgataccgg cgccgagtaa ggccaagacc gcgccgaccg acgcaaaatg
     5161 ggcatcgaca cccaggcagg catagctggc gatgccggca acaatcaacc cgatcccgat
     5221 ggttgccacc gacgcggcgt cgaagcgatc ggccaagcgg ccgctcagcg gcgccgccaa
     5281 ggccgtcgat aagggcaaga tcgccagtag aaatccagcg gcgatggcgc tggcgccaag
     5341 cacgttttgc aaataaagcg gcgccgccaa ggcgatcgga ttgtgcagta tatgactgag
     5401 gatcagggcg atcatagcgg cagacagcat tttattgcga aagatcttca cgtcgaccaa
     5461 cggcttcgcg gtgcgcagt
//
LOCUS       DASQKS010000341         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3960327, whole genome shotgun sequence.
ACCESSION   DASQKS010000341 DASQKS010000000
VERSION     DASQKS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3960327"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VE170_08870"
     CDS             complement(<1..1477)
                     /locus_tag="VE170_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ15589.1"
                     /translation="MAELTVVGKPVTRVDANEKVTGQIVYGYDLALPRMLYGKVLFSP
                     KAHAKIIRIHTDKAKALPGVVAVVTGADAPWTHGEAVKDKPFLAQGKVRYIGEPVAAV
                     AAEDEDTAQAAVKLIEVEYEDLPVYTDPEAACKPGCMEIHEDYDSYRKANFMVRGSAP
                     NVAEHFKLRTGDVAKGFAESDLVLEERYFVPMIQHAAMEPHSAHAQWDKDSGRLTIWV
                     ANDAPFRALHEISEALDMAKEKIRFINPAQGGGFGSKGGLKCEPIAVALAFHTNGRPV
                     RVKFNREETFISTLTRHEAVVYSKTGVKKDGTLMAREMTIYWGAGAYAEKSPTVCIRG
                     SLPAPGPYRIPHVKVDGYAVYTNKPVAGSYRGYGIPQGAWAGEQQMDELAKKLGMDPI
                     DIRMKNIFVEGDTSYWGEKLHAVGLKETLVKATEAIDYGKPSTVNKPGLKIGKGFACI
                     QKPTRSPTTSNAGVTVNAQGEVTVFAGTVEIGQGCNTILSQV"
     gene            complement(1545..2024)
                     /locus_tag="VE170_08875"
     CDS             complement(1545..2024)
                     /locus_tag="VE170_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ15590.1"
                     /translation="MKTKVQFTLNGKSLSREVQTHRLLLDLLRDEIGATGTKEGCGTG
                     DCGACTVMMNGKPVNSCLILSGELDGADIITIEGLKIGPELHPVQQAFVQDGGAQCGY
                     CTPGMLMMSKALLDENPNPSEEEIRYALSGNLCRCTGYAKIVQAVQDAAVMLRAKTP"
     gene            complement(2138..3049)
                     /locus_tag="VE170_08880"
     CDS             complement(2138..3049)
                     /locus_tag="VE170_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYJ15591.1"
                     /translation="MKRTEAFEFFQPATLQEASRMLKDNGPGGRFLAGGTDLVIAMKE
                     KGLVPRYIVDLKRVPGLAGIRENSDGSITLGALTTLYTIETSALIRQKYPFLAQSAAE
                     VGSIQIRNRATIGGNMANASPSADTSPALVALGASATIANGAGARTVAVEDFFKGPGQ
                     SVMNADDILSEITIPKTAANLVGEYIKFSPREMMDLAYVGVAVAYSLGASDKKCAGVR
                     IVLGAVAPTPIRAKRAEAALEGQVLSEALAEKVGRIAAEEAKPISDVRSSADYRRAMV
                     GTMTKRALLNAAAGPAKSWYDRRDRRY"
     gene            complement(3215..4585)
                     /locus_tag="VE170_08885"
     CDS             complement(3215..4585)
                     /locus_tag="VE170_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HYJ15592.1"
                     /translation="MAADLVIKGGWVVTPDATFKGGVAISNQVIVAIGDDASLPEGKE
                     VIDVKGKHILPGLFDGHVHFREPGMTHKEDFNTGSTAAVCGGITTVVDMPNTVPPTAD
                     AEQVKIKKELGEAKSLVDFGITGVVVQTNTKDILPMAAAGVIGYKIFFGETIGNLPFP
                     DDGMCVEAFDYIAQSKLPLGIHAENRQIMAYNTNKLKTEGKNEARYWEASRPDICEAE
                     SVHHAIFFAETFGTKLHVFHMSSKQAAYMVRDAKARGMRITAETGPHYLLREPNDMDK
                     VGSLLKMNPPVRTKDHAEVLWQGLREGFVDTLATDHSPHTLEEKGSDIYGKLTKPAIW
                     DCISGFCGVETGVPLILTEVNKGRLTLNQYVKVASENPAKVWQVYPKKGAIRLGSDGD
                     ITVVDMDKEGTIDVNKLHSKNKPSPWHGWKVKGMPVLTVVRGHVQMRDGEPCGKAIGK
                     ALKPMV"
     gene            complement(4617..5336)
                     /locus_tag="VE170_08890"
     CDS             complement(4617..5336)
                     /locus_tag="VE170_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYJ15593.1"
                     /translation="MNFPLKKSKTKTPAVENLSARVYHQVKQLILCNEIMPGQKLHHQ
                     QLSERLGVSRTPVREALTRLGQEGYVSFLPNRGFTCKEIRIQEAEELYDLRESLEAFA
                     VEKAVEKLTDQALAELRQRLDLYGHDVQNRFTRERLVYDQDVHLHIAEIAGNETLKNT
                     LSHVFERIVLKRRTDGIYDPARGLTAHQEHTELLEAMLRRDARSAVAIMRNHIQAGKK
                     NVIADLRQRQAIRELRPAELI"
     gene            5538..>5776
                     /locus_tag="VE170_08895"
     CDS             5538..>5776
                     /locus_tag="VE170_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ15594.1"
                     /translation="MAQTKLQAVGLRHEYYQPRTGGRLLALDQINLEVNEGEFVTIVG
                     PSGCGKTTFINLCDGLLKPTAGHITIDGKTVTGPG"
BASE COUNT         1073 a         1561 c         1752 g         1390 t
ORIGIN      
        1 ctacctgtga gaggatcgta ttgcagcctt ggccgatttc cacggtgccg gcgaacacgg
       61 taacttcgcc ttgagcgttg acggtgacgc cggcattgga agtggtcggc gagcgggtcg
      121 gtttttggat gcacgcgaag cctttgccga ttttaaggcc cggcttattg acggtcgacg
      181 gcttgccgta atcgatcgct tcggtggcct tgaccagggt ttctttcaag ccgacggcgt
      241 ggagtttctc gccccagtat gaagtgtcgc cttcaacgaa gatatttttc atgcgaatgt
      301 cgatggggtc catgccgagt tttttcgcca gctcgtccat ctgctgttcg ccagcccagg
      361 cgccctgggg aatgccgtag ccgcgatagg agccggccac cggcttattg gtataaaccg
      421 cgtagccgtc gactttgaca tggggaattc tatagggtcc cggagccggc agactgccgc
      481 ggatgcacac cgtcgggctc ttttcggcgt aggcgccggc gccccaatag atcgtcatct
      541 cgcgcgccat cagagtgccg tcttttttca cgccggtctt cgagtacacc accgcctcat
      601 ggcgggttaa ggtcgagata aaggtctcct cgcggttgaa cttcacgcgc accggccggc
      661 cgttggtatg aaatgccagg gcgacggcga tgggttcgca tttgaggccg cccttggaac
      721 cgaagccgcc gccttgggcc ggattgatga agcggatttt ctccttggcc atatcgagtg
      781 cttcggagat ctcgtgcagg gcgcggaacg gcgcatcgtt ggctacccaa atggtcagac
      841 ggccggaatc tttatcccac tgcgcatggg ccgaatgggg ctccatggcg gcatgttgaa
      901 tcatcggcac gaaatatctc tcttcgagca ccaggtcgga ctcggcgaaa cccttggcga
      961 catcgccggt gcgcagtttg aaatgttcgg cgacattggg cgcgctgccg cgaaccataa
     1021 agttggcctt gcgatagctg tcgtagtctt cgtggatctc catgcagccg ggtttgcagg
     1081 ccgcttcagg atcggtgtaa accggcaggt cttcatattc gacttcgatg agtttcaccg
     1141 ccgcttgggc ggtgtcttcg tcctcggcgg ccaccgccgc caccggctcg ccgatataac
     1201 gtactttgcc ctgggccaag aacggcttgt ctttgaccgc ctcgccgtgg gtccaaggcg
     1261 cgtcggcgcc agtgacgacg gctacgacgc cgggcagcgc tttggccttg tcggtatgga
     1321 tgcggattat ttttgcatga gccttggggc tgaacagcac cttgccgtaa agcatgcgcg
     1381 gcagggcgag atcgtagcca tagacgattt gtccggtaac cttttcgttg gcatcgacgc
     1441 gcgtgaccgg tttgccgaca actgtgagtt ctgccatggt tcgctccaaa aattagttca
     1501 agggttcaag gttcaagggt tcaaggtcag agctgtgctg ccactcacgg ggttttcgcg
     1561 cgcagcatga ccgccgcatc ttggaccgct tggacgattt tggcgtagcc ggtgcagcgg
     1621 cagaggttgc ccgagagggc gtagcgaatt tcttcttcac tcggattggg gttttcatca
     1681 agcaaggctt tggacatcat caacatgccc ggcgtgcagt agccgcattg ggcgccgcca
     1741 tcttggacga aggcttgctg caccggatga agttcggggc cgatcttcaa gccttcgatg
     1801 gtgatgatat cggcgccgtc cagttcgccg gaaagtatca gacaggagtt caccggcttg
     1861 ccgttcatca tcacagtgca ggcaccgcaa tcgccggtgc cgcagccttc cttggtgccg
     1921 gtggcgccga tttcatcgcg cagcaggtca agcaggagtc gatgggtctg gacctcgcgg
     1981 ctgagtgatt tgccgttgag ggtgaattgg acttttgtct tcataagtaa ctccaagttc
     2041 agcttggagt gttggagtga cggagtactg gagtgttggg tcgcggattc cggaaaccca
     2101 tcactccatc attccattac tccatcactc catgatgcta gtatcttcga tcgcgccggt
     2161 cgtaccagga tttcgctggg ccggcggcgg cgttgagcag ggcgcgtttg gtcatggtgc
     2221 ccaccatggc gcggcgataa tcggccgacg agcgcacatc gctaatcggt ttggcctctt
     2281 cggcggcgat ccggccaact ttctccgcca gagcttcgct gagaacttgg ccttcgagcg
     2341 ccgcttcggc gcgcttggct ctgattggcg tcggcgccac ggcgccgagg acgatgcgta
     2401 cgccggcgca tttcttgtca ctggcgccga gactgtaagc gacggcgacg ccgacgtacg
     2461 ccaggtccat catttcgcgt ggtgaaaatt ttatgtactc gccaaccagg ttggcggcgg
     2521 tttttggaat ggtaatctca ctgaggatgt catcggcgtt catcacgctc tggcccggcc
     2581 ctttgaagaa atcttctacg gcaacggttc ttgcgccggc tccgttggcg atggtcgcgc
     2641 tggcgccaag cgctaccagc gccggtgaag tatcggccga cggcgaagcg ttggccatgt
     2701 tgccgccgat ggtggcgcgg ttgcgaatct gaatcgagcc aacttccgcc gcgctttggg
     2761 cgagaaacgg atatttttgc cggatcagtg ccgaggtctc aatggtatac agggtcgtca
     2821 acgcgcccaa ggtaatactg ccgtcgctat tttcgcggat gccggcgagg ccgggcacgc
     2881 gcttcaagtc aacgatgtac cttggcacca accctttctc tttcatggcg atgaccaggt
     2941 cggtgccgcc ggcgagaaag cgtccgccgg gaccgttatc cttgagcatc cggctcgctt
     3001 cctggagcgt agccggctgg aaaaattcaa atgcttctgt gcgtttcatg aaactaattc
     3061 ccttgccgag tccgagcact gccagggcgt aacttcggtt aatttaatgc ctagttagga
     3121 ggagagtatt ggagtgatga aggagtggag tttggcgccg gaaataatcc gaaaaccatc
     3181 gctccaattc tccatcactc caattcgccg attttcagac catgggcttg agtgctttgc
     3241 cgatcgcttt gccgcagggc tcgccgtctc tcatttgcac atgaccgcga acgaccgtga
     3301 gcactggcat gcctttgact ttccagccat gccaaggcga gggtttgttt ttgctgtgca
     3361 gcttgttgac gtcgatggtg ccttctttat ccatgtcgac aacagtgatg tcgccgtcgg
     3421 agcccaaacg gatggcgcct ttcttaggat agacctgcca gactttggcg gggttctcag
     3481 acgccacttt gacgtattga ttcaaggtca agcggccttt gtttacttcg gtcaagatca
     3541 gcggcacgcc ggtctcgacg ccgcagaagc cggagatgca gtcccagatc gccggcttgg
     3601 tgagcttgcc gtagatgtcc gagcccttct cctccaatgt atgcggcgag tgatcggtgg
     3661 ccagggtgtc gacgaagcct tcgcgcaatc cctgccagag cacttcggcg tgatccttgg
     3721 tgcggaccgg cggattcatc ttcaacaacg aaccgacttt atccatgtca ttgggctcgc
     3781 gcagcaagta atgcgggccg gtctccgcgg tgattctcat gccgcgcgcc ttggcgtcgc
     3841 ggaccatata agcggcttgc ttcgagctca tgtgaaatac atgcagcttg gtgccgaagg
     3901 tttcggcgaa gaagatcgca tgatgcaccg attccgcttc gcagatatcc ggccgggagg
     3961 cttcccagta gcgcgcttcg ttcttgcctt cggtcttcag tttgttggtg ttgtaagcca
     4021 tgatctggcg gttttcggcg tggatgccca aggggagctt cgactgggcg atgtaatcga
     4081 acgcttcgac gcacatgccg tcatcgggga agggcagatt accgatggtc tcgccaaaga
     4141 agatcttgta accgatgacg ccggcggcgg ccatgggtag aatgtccttg gtgttggtct
     4201 gcacgaccac gccggtgatg ccaaagtcga ccaaagattt ggcttcgccc agttctttct
     4261 tgattttgac ctgttcggcg tcggccgtgg gcggcacggt gttcggcata tcgacgacgg
     4321 tggtgatgcc gccgcagacc gccgcggtgg agccggtgtt gaaatcctct ttgtgcgtca
     4381 tgcccggttc gcggaaatgg acatggccgt cgaataagcc cggcaggata tgtttgcctt
     4441 tgacgtcgat gacctctttg ccttcgggca gtgaagcatc gtcgccgatg gcgacgatca
     4501 cttggttgct gatcgccacg ccgcctttaa atgttgcgtc gggtgtcacc acccaaccgc
     4561 ctttgataac tagatctgct gccataattc tccctttctt ctgttggttt gcggtcctag
     4621 attagttccg caggccggag ctcgcggatc gcttggcgtt gtcgcaggtc ggcaatgaca
     4681 tttttcttgc cggcttggat atggttacgc atgatggcga cggcggagcg cgcgtcgcgc
     4741 cgcaacatcg cttcgagtag ctcggtgtgt tcttgatggg ccgtgaggcc gcgcgccggg
     4801 tcgtaaatgc cgtcggtgcg gcgcttgagc acgatgcgct cgaaaacatg gctcaaggta
     4861 tttttcaggg tctcgttgcc ggcgatttcg gcaatgtgga gatggacgtc ctggtcgtag
     4921 accagccgct cccgggtgaa gcggttttgc acgtcatggc cgtagagatc cagccgctgg
     4981 cgcaactcgg cgagggcctg gtcggtgagt ttctcgacgg ctttttcgac ggcgaaagcc
     5041 tcgagggatt cgcgcagatc gtagagctcc tccgcctctt gtattctaat ttctttgcag
     5101 gtaaaacccc ggttcggcag aaaggaaacg tacccttcct gaccgagtcg agtcagcgct
     5161 tcgcgcaccg gagtgcgact gacgccgaga cgttcgctga gttgttgatg atgcagcttt
     5221 tgtcccggca tgatttcatt gcacaggatc aactgtttaa cttggtggta aacgcgggct
     5281 gagaggtttt cgacggcggg cgttttggtt ttagattttt tgagcgggaa attcatcgga
     5341 aaaattttgc ataaaatatt ttatgcagga gcgaaaatta tccgatccgc tttggcttgt
     5401 caacgaagag atctttttgc ttgaataaac ggtggaaaat gataaggagt catgcgaccc
     5461 aagcgccgaa cccgcttgcg gataattttt caacgagcat aagtgcggca taacgcgaat
     5521 atattttagg aggcattgtg gcacagacaa aactacaggc agtcggacta cggcatgagt
     5581 attatcagcc gagaactggt ggcaggcttt tggccttgga ccagatcaac ctcgaagtga
     5641 acgaaggcga attcgttacc atcgttggac ccagcggttg cggtaaaacc acatttatca
     5701 atttgtgcga tgggctgctc aagccgacgg cggggcatat taccatcgac ggcaaaaccg
     5761 tgaccggacc cggtat
//
LOCUS       DASQKS010000342         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3119258, whole genome shotgun sequence.
ACCESSION   DASQKS010000342 DASQKS010000000
VERSION     DASQKS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119258"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VE170_08900"
     CDS             <1..852
                     /locus_tag="VE170_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15595.1"
                     /translation="TKEAGIFEKHGLYVDPVYVASGTKVAQAVIAGEFPVALAGGTVV
                     NATLAGGDIVIIGGLVNVPSFYVYVHPSVKRPEDLKGKAIGITTYGSSTDFSIRYLIK
                     KWAMEPDRDIKVLQMGGQPQIVAAMVGGSVQGGVLSSPANYRAAKAGFQLLTDFKKVG
                     LDYPTVSLVSTRSFIKKDPQTVRRFMMAYSEGVERLYRDKEFSMKVIGKYTKTDDREA
                     LDSAYGFATTFIERPPHLPFKAIETILAQLGEKDPKAREAKAEDFIDATFYNEMEKSG
                     FFKSSAR"
     gene            1035..2078
                     /locus_tag="VE170_08905"
     CDS             1035..2078
                     /locus_tag="VE170_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ15596.1"
                     /translation="MKAIIIRQFGAPEVLRLEEVAEPKAAPDEIVIKVQSVSVNRTLD
                     CVVRAGKYPVTIQMPHVLGVDPAGEVVEVGSEVARFKIGDRVATISIIRCGRCKQCAR
                     GREANCLASQHIGLHRWGGYAEYVAVPERNAFKIAENLTYAEGAVITRHYPMAFNLLT
                     GKTQVQAGEWVLVMGATGALGSCCVQVAKMFGAKVIAAAGADARVELAKQSGADFGIN
                     YRTQDLAKEVMALTAGDGVDIVCESIADPTLWPGAFNSLAIGGRMVTAGAHGGGNVLL
                     DAKRLYQRRISILGAAGTNPPDVDKALEAARAGKIKAMPHRKMALSQAAEAHRIVENN
                     QIIGKIILEPGKD"
     gene            2223..3074
                     /locus_tag="VE170_08910"
     CDS             2223..3074
                     /locus_tag="VE170_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HYJ15597.1"
                     /translation="MAQIVSIIGITHNPFMPRLFKQAQQPPGAVKVKERIAMMREKLA
                     AAKVDVLVCIGNDHLHQFFMDNMPAFMIGKMEQYDGTFYDEEREFGLPKCKLQGDLDV
                     SDAIMEGAFDRGVDFSYSNELTIDHSIVVPMMFVRPEMDIPIVPILTNCIAPPMPRAK
                     RYFEVGKAIRAAIDSLPTNKRIGVLVSGHLSLEIGGPKMFEPRLTDPNFDANAVGWIV
                     NGNIDAATEACAPEKMLQYGNMTTGYLNFIMMMGVANLAKPSYAEGLDAGFPAIPFFS
                     WDQSPKV"
     gene            3092..>3344
                     /locus_tag="VE170_08915"
     CDS             3092..>3344
                     /locus_tag="VE170_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HYJ15598.1"
                     /translation="MDLKILSTVFTSVFIAELGDKTQLATMLFAADKDTSKWTVFLGA
                     AAALVVTSAIGVAAGSIVSQYVSEKTLQYLAGIGFVGIGI"
BASE COUNT          772 a         1013 c          921 g          638 t
ORIGIN      
        1 accaaagagg ccggcatttt cgaaaaacac ggtttgtatg tcgatccggt ttacgtcgcc
       61 agcggaacga aagtggcgca agcggtcatc gccggcgaat tccctgtggc gttggccggt
      121 ggtacagtcg tcaacgccac actcgccggc ggcgatatcg tcatcattgg cggtctggtc
      181 aacgtgccgt ccttctacgt ctacgttcat ccatcggtta aacggcccga agatttgaaa
      241 ggcaaagcca tcggcatcac cacctacggc tcgtccaccg atttttccat ccgctattta
      301 ataaagaaat gggccatgga acccgaccgc gatatcaaag ttttgcaaat gggcggccag
      361 ccgcagatcg ttgccgccat ggtcggcggc agcgtccaag gcggcgtgct ctcatcgccg
      421 gccaattatc gcgcggccaa agccggcttt cagctgctca ccgatttcaa gaaagtcggc
      481 ctcgactacc caactgtcag cctggtgtcc accaggtcgt ttatcaagaa agatccacaa
      541 accgtgcgcc gcttcatgat ggcgtacagc gaaggcgtcg agcggctcta ccgcgataaa
      601 gagttcagca tgaaagtcat cggcaagtac actaaaaccg acgatcgcga agccttggat
      661 tcggcctatg gcttcgcgac aacattcatc gagcggccgc ctcatctgcc tttcaaggcg
      721 atcgaaacga tcctggcaca gctcggcgag aaagatccga aagcgcgcga agccaaagct
      781 gaagacttca tcgacgcaac tttctacaac gaaatggaaa agagcggctt cttcaagagt
      841 agcgcgcgct aacgctgcaa ccgaggatgg tcctaagaca tctactggcc gtctcgctgc
      901 gaaggacact aagagcacaa agttcggaat aattatttcc gaaaccttcg tgttcggagc
      961 ctgtcctgag cgcagccgaa ggatgcctcc gtggtgagaa tgatttcccg taaacctgcc
     1021 ggcaacacgt taccatgaaa gccatcatca tcagacagtt cggcgctccc gaagtactgc
     1081 gcctagagga agtcgccgag cccaaagccg cgcccgatga gatcgtcatc aaggtccaat
     1141 cggtatcggt caaccgcacc ctcgactgcg tggttcgcgc gggcaagtac ccggtcacca
     1201 ttcagatgcc ccatgttctg ggcgtcgatc cggccggcga agtcgttgaa gtcggtagcg
     1261 aagtagcccg attcaagatc ggcgaccgcg ttgccacgat atcgatcatc cgctgtggcc
     1321 ggtgcaagca atgcgcgaga ggccgcgaag cgaattgttt ggcgagccag catatcggcc
     1381 tgcaccgctg gggcggctac gccgaatatg tcgctgtgcc agagagaaac gccttcaaga
     1441 tcgccgagaa tttaacctat gccgaaggtg ccgtcatcac ccgccactat ccaatggcgt
     1501 tcaacctgct caccggcaag acccaggttc aagccggcga atgggttttg gtcatgggcg
     1561 ccaccggcgc gctcggcagt tgctgcgtcc aggtggcgaa aatgttcggc gccaaggtga
     1621 tcgccgccgc cggcgccgac gcgagagtcg agttggcgaa gcagagcggc gccgacttcg
     1681 gcatcaacta tcgcactcaa gatctcgcca aagaggtcat ggcactcacc gccggcgatg
     1741 gcgtcgatat cgtctgcgaa agcattgccg acccgacgct ctggcccggc gcgtttaaca
     1801 gccttgccat cggcggccgc atggtcaccg ccggcgccca tggcggcggc aacgtgctgt
     1861 tggatgccaa gcgtttgtac cagcgccgca tcagcatcct cggtgccgcc ggcaccaatc
     1921 cgccggacgt ggacaaagca ctcgaagcgg cacgcgccgg caagatcaaa gcgatgcccc
     1981 accgcaaaat ggcgctgagc caagccgccg aggcgcaccg cattgtcgag aacaatcaga
     2041 tcatcggtaa gattatcctc gaaccgggga aggattaaga tttgcaccag caaagcgcta
     2101 ggtttggaaa aacttaggga actttgtgcc tttgcaggag acaaatccga atccgccgcg
     2161 gcgtttacac tagacaagcc atcatgctaa acagcaaatg actcgttcga taggaggaaa
     2221 ccatggccca gatcgtttcg atcatcggca tcacgcacaa tccctttatg ccgcgtctat
     2281 tcaaacaagc gcagcaaccg cccggcgccg tcaaagtcaa agaacgcatc gctatgatgc
     2341 gcgagaaact agccgcagcc aaagtcgacg tgctggtgtg catcggcaat gaccatctgc
     2401 atcaattttt catggacaac atgccggcgt ttatgatcgg caagatggag cagtacgacg
     2461 gcacttttta cgacgaggag cgcgagtttg gcttgccgaa gtgcaagctc caaggcgacc
     2521 tcgacgtctc cgacgccatc atggagggcg ccttcgaccg cggcgtcgac ttttcctatt
     2581 ccaacgaatt gaccatcgac cactcgatcg tggtgccgat gatgttcgtc cggcccgaga
     2641 tggatattcc catcgtgccg atcttgacca actgcatcgc gccgccgatg ccgcgggcaa
     2701 aacgctactt cgaggtcggc aaagcgatcc gcgccgccat cgacagcctg ccgaccaaca
     2761 aacgcatcgg cgtgctcgtc agcggccact tatcgctgga aatcggcggc ccgaaaatgt
     2821 tcgagccgcg cctcaccgat ccgaacttcg atgccaacgc ggtgggctgg atcgtcaacg
     2881 gcaacatcga tgccgccacc gaagcttgcg ccccggaaaa gatgctccaa tacggcaaca
     2941 tgaccacagg ctatttgaac ttcatcatga tgatgggcgt ggcgaatttg gccaaacctt
     3001 cctacgccga aggcctggac gccggattcc cagccatccc gttttttagc tgggatcaat
     3061 cgcccaaagt ttaatgatcc agaggcccgc aatggattta aaaattctct ccactgtgtt
     3121 cacgtccgtc ttcatcgccg agctcggcga caagacccag ttggcgacga tgctattcgc
     3181 cgcggacaag gacaccagca aatggacagt cttcctcggc gccgccgcgg ctctggtcgt
     3241 cacctccgcc atcggcgtcg ccgcgggctc gatcgtttcc cagtatgtca gcgagaaaac
     3301 cttgcagtac ctggcgggaa tcggctttgt cggaatcggg atat
//
LOCUS       DASQKS010000343         8145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3960416, whole genome shotgun sequence.
ACCESSION   DASQKS010000343 DASQKS010000000
VERSION     DASQKS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3960416"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VE170_08920"
     CDS             <1..185
                     /locus_tag="VE170_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15599.1"
                     /translation="AKMPAKPYAVESAVQAVLDHLAESQPKYGQFKPGEFIDARTLSD
                     IDKSGYIDKLYGGQAR"
     gene            198..2240
                     /gene="uvrB"
                     /locus_tag="VE170_08925"
     CDS             198..2240
                     /gene="uvrB"
                     /locus_tag="VE170_08925"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HYJ15600.1"
                     /translation="MLRDQDSRQFKLVSEFQPEGDQPQAIDKLVKGLTGKRRKQVLLG
                     VTGSGKTFTMANVIARVNRPTLVIAPNKTLAAQLYEEFRTLFPDNAVRYFVSYYDYYQ
                     PEAYLPASDTYIEKDSSINDEIDKLRHSATKALLERRDSLIVASVSCIYGLGSPEAYF
                     DLMVYVEQGATINRDQMLRKLVDIQYQRSDYDFHRGTFRVRGDIVEIFPAYEDRSALR
                     IEFFDETIESISEIDPIRGKILRRVDKATIYPASHYVTGEARRKQAVLAIRMELKARL
                     AELQEQHKSLEAQRLQQRTLYDLELLEEMGFCPGIENYSRHLTGRQPGEPPPTLLSYF
                     PNDFLLMIDESHVTVPQIGGMYRGDRSRKETLVEYGFRLPSALDNRPLNFEEFAALID
                     QTIYVSATPAPYELRESNGAVIEQVIRPTGLMDPAILVRPARTQVDDLLEEIRKRVER
                     KERVLVTTLTKRMAEDLTEYYQDLNVKVRYLHSDIETLERVEIIRQLRSGQFDVLVGI
                     NLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGTVILYADGVTDSM
                     KRAMDETERRRRLQAAFNKKHGITPQTIVKALSSPLVKIYDADYADIPLAAEGSPQYG
                     PGELTRRLRKLQKEMKEAAQKLEFERAAELRDQIRQLQSQELTLRDSQLDSRVDSD"
     gene            complement(2251..2670)
                     /locus_tag="VE170_08930"
     CDS             complement(2251..2670)
                     /locus_tag="VE170_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15601.1"
                     /translation="MFEIETLMQLGVFSQLIPIWQVFFFIAALLPFLLMDRVKICLLV
                     TYLFTFYLGFMVQWGEYLSSTGSMQPFVLYSLSGLAIAVFFVATIFNDPARGPNARGV
                     RYGQAHGNPGEAPIAAARRDPVEIAVDVTEVSEPERH"
     gene            2917..4794
                     /gene="uvrC"
                     /locus_tag="VE170_08935"
     CDS             2917..4794
                     /gene="uvrC"
                     /locus_tag="VE170_08935"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HYJ15602.1"
                     /translation="MNTLTEKLAGLPSRPGVYLMRESGGKVVYVGKAKDLRARVRAYF
                     HHPDERSQIQFLVRRIADIETLVTSNDKEALILENNLIKQYKPRYNIRLKDDKSYLSI
                     KVTTQHAWPRILPTRKIVKDGNRYFGPFSSALAARETIDLIEKHFQLRNCTDHNFRNR
                     SRPCLQYQIKRCLGPCVLPVAHDVYLEHVRQTMLFIEGRQQELIDELKQRMREKAETQ
                     EFEAAARLRDQIQAVEKTLEKQRMVAHWGSDQDIFGLYREGGFIEVQVLLVRQGKLTG
                     SQSYSLADLEFPDDEIMGSLLTQFYQGQRFVPDEILLPVDLEDASVREEYLAEHKGKS
                     VKILCPQRGDKRHLVAMAVDNAKQSFADRHDQEKAREGMLRELQTSLRLKNYPQRIEC
                     FDISMIHGAHAVGSMVTFIGGQPDKNFYRHFRIKTIGAESGGDDFGMMLEMLKRRFSR
                     GKAEGDLPELIVVDGGKGQLAMALAAMAELGIDGVEAVGLAKMRVQAAPRSAEIERSE
                     ERVFLPGQTNPVILRRNSNALFLLQRVRDEAHRFAITHHRKLRSQQTLYSALDRIPGV
                     GGVRKRALLRAFGSIKRIEEASLEDLLKVPSMNEKLAQQVLDALHGHTQAENSPQSVV
                     N"
     gene            4926..7400
                     /locus_tag="VE170_08940"
     CDS             4926..7400
                     /locus_tag="VE170_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HYJ15603.1"
                     /translation="MADQQDVAGLRSGGFHPSAWLIVPTLFLLLGQALAAAPWHLPDS
                     SGLALVPFLLLPFYRCRRRWVMLGLLVTLAFSVGYVRHLEILFPQFSNAHLRSVMLRH
                     ERVLIEGRLKAEPEKLINRHRWSMRAERIWHAAGAEEVSGDLQLTLRVMRRDWRYGDR
                     IRFWLRPSLPQNSGNPGGFEYATYLAQRQIYVIGYVENDAEIELVAREPGLIRGTIED
                     IRRQIRGYIDANFAPDSGALMKALTVGEMGGISKEMRTSFTAAGINHVLSISGLHVAM
                     LGVVIFAAIRYGLSFSSYLLLRFNLLKAATFFAFLSVVFYTALAGAMVPTVRSAIMIG
                     VYQLAVLLDREEEVFASLTLAALGIALVWPGVIADISFQLSFLAVLFIAWGMSRLYHG
                     LPRQKPEELPQEKSWIKERARRAIFHLAVPLLATLGTGPLIAHYFGNVSLAGFVTNPL
                     IVPLVGFVIVPLGLTIGLVTLIASEFAWPLAWLGDRLAGWTIVIVEFAGRLPLASIRV
                     AAPNLLEVGALYAGVIALFVLRKRRQLFFAAGAFLLVLVGDVYYWRVQRFDSNQLRVT
                     HLNVGQGDAAVVEFPGGKILVIDAGGAAVGDFDTGESIVAPYLRARKILKIDYLLVSH
                     ARIDHYGGMRALVQEFSPAEFWFGTAIGGTRRLEELDETLNRFNIARKELTGTTPCRN
                     FDALRLCVLFAGHGTTEGPVVVRLEYGKASFLFTSDVDKREELQLMQSSSAIGSTVVK
                     IPRHGSATASSQDFIAAVAPKLAILSAGARSRAEMKRDEIVERYHAAGAEVLKTYEDG
                     AIILETNGNTLRYSGFKSGKAGMLVL"
     gene            complement(7597..>8145)
                     /locus_tag="VE170_08945"
     CDS             complement(7597..>8145)
                     /locus_tag="VE170_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15604.1"
                     /translation="NPIDEGFRRGPGEAYGNSKVAKELMVEAYQRMYGFELIVLRLAN
                     VYGVGHFAGGSGGGEMVQNMLQTGIKGGVVKVPQEVARDFEYVYYKDLGRALDKAVTT
                     PLKEPVTLNIGTGVVIPFDDLVATAEKLLPNLQVEIIPGIKPRSAKQPMVIEKAKQIL
                     GWTPEYDIVNGFKDYIGELKAL"
BASE COUNT         1717 a         2222 c         2446 g         1760 t
ORIGIN      
        1 cggcaaaaat gccggccaag ccttacgcgg tcgagagcgc cgtgcaggcg gtgttggatc
       61 atctagcgga aagccagccc aaatatgggc aattcaaacc cggcgagttc atcgacgcac
      121 gcacgctcag cgacatcgac aagagcggtt acatcgataa gctttacggc ggccaggcgc
      181 gataggttca aggcgcgatg ttacgcgatc aagacagccg ccagttcaag ctggtgtcag
      241 agttccaacc ggagggcgat caaccgcagg cgatcgataa gctggtcaag gggctcacgg
      301 gcaagcgccg taagcaggtt ctcttgggag taaccggatc gggtaagaca tttacgatgg
      361 ccaacgtcat cgctcgggtg aaccgaccga ccctggtcat tgcaccgaac aaaacgcttg
      421 cggcacaact ctatgaagaa tttcgcacgc tgtttcccga taatgcagtg cgctacttcg
      481 tcagctatta tgattactac cagcccgagg cctacctgcc ggccagcgat acttatatcg
      541 agaaggattc gtcgatcaac gacgagatcg acaagctacg ccactcggcg accaaggcgc
      601 tgcttgaacg gcgcgacagc ttgatagtcg ccagcgtctc gtgcatctac ggcttgggtt
      661 cgccggaagc ttactttgat ctgatggttt acgtcgaaca gggcgccacg attaatcgtg
      721 accagatgct gcgcaagctg gtcgatattc agtatcaacg cagcgattac gattttcacc
      781 gcggcacttt tcgcgtgcgc ggcgacattg ttgaaatatt tcccgcctac gaagatcgtt
      841 cggcgctgcg catcgagttt ttcgacgaaa ccattgagtc gatcagcgag atcgatccga
      901 ttcgcggcaa gatcttgcgg cgcgtcgaca aggcgacgat ttatccggcg agccattacg
      961 ttaccggtga agcccgccgc aagcaggcgg tcctggcgat tcgcatggag ctgaaagcgc
     1021 gcctagccga gctgcaagag cagcacaaat ctttggaagc gcagcgcttg caacagcgca
     1081 cgctttacga tctcgaactg ctggaagaga tgggcttctg ccccggtatt gaaaattatt
     1141 cgcgccatct gaccgggcgc caaccggggg agccgccgcc gacattgtta agttattttc
     1201 ccaacgattt tcttctgatg atcgatgaga gccatgtgac tgtgccgcag atcggcggca
     1261 tgtaccgcgg cgaccgctcg cgcaaggaaa ccttggtaga atacggcttt cgcttgccgt
     1321 cggccctgga caaccggccg ctaaatttcg aggagttcgc ggcgctgatc gatcagacga
     1381 tttacgtctc ggcgacgcct gcgccgtacg agctgcgcga gagcaacggc gcggtgattg
     1441 agcaggtgat tcgtccaacg ggtttgatgg acccggcgat cctcgtgcgc ccggcgcgca
     1501 cccaagtcga cgatcttttg gaggaaatcc gtaaacgggt ggagcgcaag gagcgcgtgc
     1561 tggtaacgac gctgaccaag cgtatggccg aggatttgac cgaatattat caggatctca
     1621 acgtcaaggt ccgctacctg cactcggata ttgaaaccct cgagcgggtc gagatcatcc
     1681 gccaactgcg cagcggccag ttcgacgtct tggtcggcat caatctactg cgcgagggat
     1741 tggatttgcc ggaggtatcg ttggtggcga ttctcgacgc cgacaaagaa ggctttctgc
     1801 gctcggagcg atcgttgatc caaaccatcg gccgggccgc gcgcaacgtc aacggcacgg
     1861 tgattctcta cgccgacggt gttaccgact ccatgaagcg cgccatggat gaaaccgaac
     1921 ggcggcggcg attgcaggca gcgttcaaca agaagcatgg catcaccccg caaaccatcg
     1981 tcaaggcgtt gagttcgccg ctggtgaaaa tatacgacgc tgattatgcc gatattccgc
     2041 tggcggccga gggatcgccg caatatggcc caggggagct tacccggaga ttgcgcaaac
     2101 tgcagaagga aatgaaagaa gcggcacaaa agttggagtt tgagcgggcg gcggagttac
     2161 gcgatcagat ccgccagctg caaagccagg agttgacctt gcgcgattcg cagctcgact
     2221 cccgcgtcga tagcgactag acaccgtaga ttagtgccgt tcgggctcgg aaacctcggt
     2281 gacatcaacc gctatttcca cggggtcgcg gcgcgctgcg gcgatgggtg cttcgccggg
     2341 gttgccgtgc gcttggccgt atcggacgcc gcgcgcattt ggaccgcggg ccggatcgtt
     2401 gaagatggtg gcgacgaaga acaccgcgat ggcgagcccg ctgagcgagt agagaacgaa
     2461 gggttgcata gagccggtgg aggatagata ttcgccccat tgaaccatga agccgagata
     2521 gaaagtaaat agataggtaa ccagcaggca gatcttaacc cggtccatta gcaggaacgg
     2581 cagcagtgca gcaatgaaaa agaacacctg ccagatagga atgagttggc taaacacacc
     2641 caattgcatc agggtttcga tttcgaacat gtcggctcct tggtccttgg cctgtcgtga
     2701 atgctgcgtg attggccggg tatttaccca agagttatgc caacgatgct ttggcgcaaa
     2761 tgctgtacgg ctaggtaacg atttgtcagg accagtgact ttgatccgcg ccggcgaatt
     2821 ttccggtcac ctctgggtga tgcgattcgt gactcgcttg ttctttcgca accggtggtt
     2881 gtaccgaaac cgccgatcca aattggggat cgcgccatga acacactgac ggaaaagctc
     2941 gccggtttgc cgtcgagacc gggcgtctat ctcatgcgcg aaagtggcgg caaggtcgtg
     3001 tatgtcggca aggccaagga tctgcgagca cgggtgcggg cttacttcca ccatcccgat
     3061 gagaggtcgc agattcaatt tttagtgcgc cggatcgccg acatcgaaac tctggtgacg
     3121 agcaacgaca aggaagctct gatcctggaa aataatctga tcaagcaata caagccgcgc
     3181 tacaacattc gcctcaagga cgacaagagc tatttgagca tcaaagtgac gacgcagcat
     3241 gcctggccgc gtatcctgcc gacgcggaag atcgtcaaag acggcaaccg ctacttcggg
     3301 ccgttttcgt cggcgctggc ggcgcgcgag accatcgact tgattgaaaa acattttcag
     3361 ctgcgcaact gcaccgacca taactttcgc aaccgcagcc ggccctgttt gcagtaccag
     3421 atcaagcgct gcctggggcc ctgtgtcctg ccggtggccc atgatgttta cctggaacat
     3481 gtgcgccaga ccatgctgtt tatcgaaggg cggcagcagg agctgatcga tgagttgaag
     3541 cagcgcatgc gggaaaaggc ggagacccag gagttcgagg cggcggcaag attgcgcgat
     3601 cagattcaag cggtcgaaaa gactttggag aagcagcgca tggtcgcgca ctggggcagc
     3661 gaccaggata tcttcggctt gtaccgcgag ggcggcttca tcgaagttca ggtgctgttg
     3721 gtgcgccagg gcaagcttac cggtagccag tcttattcct tggcggattt ggaattcccc
     3781 gacgatgaga tcatgggatc gctcttgacc cagttctatc agggccagcg attcgttccg
     3841 gacgagatac ttttgccagt ggatctggag gatgcgtctg tccgcgagga gtatctcgcc
     3901 gagcataaag gcaagagcgt aaaaattctc tgtccccagc gcggcgataa acgccatttg
     3961 gtcgccatgg cggtcgataa cgccaagcaa agtttcgccg atcgccacga tcaagaaaag
     4021 gcgcgcgagg ggatgctgcg cgagttgcaa acctcgctgc gtttgaaaaa ctatccccaa
     4081 cgaatcgagt gcttcgatat ctcgatgatc catggcgccc atgcggtggg ctcgatggtg
     4141 accttcatcg gcggccagcc ggacaaaaat ttttaccgtc attttcgcat taagaccatc
     4201 ggcgccgagt ccggcggcga tgacttcggc atgatgctgg agatgctcaa acggcgcttc
     4261 agtcgcggca aggcagaggg cgatctgccg gaactgatcg tcgtcgatgg cggcaaggga
     4321 caattggcga tggccctggc ggccatggcc gaactcggca tcgacggtgt cgaggcggtg
     4381 gggctggcca agatgcgggt tcaagcagcg ccgcgcagcg ccgaaatcga acgctcggaa
     4441 gaacgcgtgt tcctgcccgg tcaaaccaat ccggtgattt tgcggcgcaa ctcaaatgct
     4501 ttgtttctgc tgcagcgcgt gcgcgacgaa gcgcatcgct tcgccatcac ccatcaccga
     4561 aaattgcgct cgcaacagac tctttactcc gctctagacc gcatccccgg cgtcggcggc
     4621 gtgcgcaaac gcgccttgct gcgcgccttc ggcagcatca aacggataga agaagcatcg
     4681 ctggaagatc ttctgaaggt accgtcgatg aacgaaaaac tcgcccagca agtgctcgac
     4741 gccctgcacg gccatacgca agcggaaaat tcgccgcaaa gcgtggtgaa ctgagacgag
     4801 ctggagccgc gcaaaggttg ataaagccgg acctggcagt ggcagcgtcc gtgatttgcc
     4861 cgcgcaaatg atcgttatga gtggcggcct caagaggcag agttcctcag aaagcgaaag
     4921 ccaacatggc ggatcagcaa gacgtcgctg gattacgttc gggaggcttc catccgtcgg
     4981 cgtggttgat cgtgccgacg ctgtttctgt tgcttggcca agcgctcgcc gcggctccct
     5041 ggcatttgcc cgatagcagt gggttggcgc tggtgccgtt cctgctattg cccttttacc
     5101 gctgccggcg gcgctgggtg atgctggggt tgttagtcac cctggcgttc tcagtcggct
     5161 atgtgcgcca tctggaaatc ctattcccgc aattttccaa tgctcatttg cgttcggtca
     5221 tgctgcgcca tgagcgggtc ttgatcgagg ggcgtctcaa ggccgagccg gagaaactga
     5281 tcaatcgtca tcgttggtca atgcgcgcgg agcgcatttg gcatgccgcc ggagccgagg
     5341 aagtcagcgg tgatttacaa ctgaccctgc gcgtgatgcg gcgggactgg cgttatggcg
     5401 accgcattcg tttttggctg cgtcccagcc tgccccagaa cagcggcaat cccggcggct
     5461 tcgaatatgc gacttatctt gcccaacggc agatctacgt cattgggtat gttgaaaacg
     5521 atgccgagat cgagctggtc gcccgcgaac cgggtttgat ccgcggcacg atcgaggata
     5581 ttcgccggca gatccgcggc tacatcgacg ccaacttcgc gcccgacagc ggtgctctga
     5641 tgaaagcgct gaccgtcggc gagatgggcg gaatcagcaa agaaatgcgt actagcttca
     5701 ctgcggctgg aatcaatcat gtgttgtcga tttccggcct gcatgtcgcc atgttaggtg
     5761 tcgtgatctt cgcggcgatc cgttacggtc tgtcgtttag cagttatttg ttgctgcgct
     5821 tcaatctact caaagcggcg accttttttg cttttttgtc ggtggtgttt tatacggcgc
     5881 tcgcgggcgc catggtgccg acggtgcgct cggcgatcat gatcggggtt tatcagctgg
     5941 cggtgctgtt ggatcgggaa gaagaagtat tcgccagtct aaccttggca gcgctgggaa
     6001 tcgccctggt ctggcccggt gtgatcgccg acatttcctt tcagctttct tttctcgcgg
     6061 tgctgtttat cgcctggggc atgagccgac tctatcacgg tctgccgcgg caaaagcctg
     6121 aggagttgcc gcaagaaaaa agctggatca aggagcgggc gcggcgggcg atatttcatt
     6181 tggcggtgcc gttgctggcg accctaggca ccggcccgct gatcgcgcat tattttggca
     6241 atgtctcgct cgccggtttc gtcaccaatc cattgatcgt cccgctcgtc ggtttcgtca
     6301 ttgttcccct cggtctgacc atcggtcttg tgactctgat cgccagcgag tttgcctggc
     6361 cgctcgcctg gcttggcgat cgactcgccg gttggacgat tgttatcgtc gagttcgccg
     6421 gccgcttgcc gctggcgagt attcgcgtcg ccgcgccgaa cctgctcgag gtcggcgcgc
     6481 tttatgccgg cgtgatcgct ttattcgttc tgcgcaagcg caggcagcta ttttttgcgg
     6541 ccggggcatt cctgcttgtg ctggtcggcg acgtttacta ttggcgtgtc cagcggtttg
     6601 acagcaatca gctgcgcgtt acccatttga acgtcggtca gggtgatgct gccgtggttg
     6661 aatttcccgg cggtaaaatt ctggtgatcg atgccggcgg cgccgccgtg ggcgattttg
     6721 ataccggcga aagcatcgtc gctccctatc tcagagcgcg gaagattctt aaaatcgatt
     6781 acctgctggt cagccatgcg cgcatcgatc actacggcgg catgcgcgcg ctggttcaag
     6841 agttctctcc cgccgaattc tggttcggca ctgccatcgg tggcacgcgg cgtcttgaag
     6901 aactcgatga aaccctgaac cggtttaata ttgcccgtaa agaattgacc ggcacaacgc
     6961 cgtgccgtaa cttcgatgcg ctgcgcttgt gcgtcctttt cgccggccat ggcacgaccg
     7021 aaggtccagt ggtagttcgc ttggaatacg gcaaggcgtc gtttctattc accagtgacg
     7081 tcgacaaaag ggaagaactc cagcttatgc agagctcaag cgcaatcggc agcaccgtcg
     7141 tcaagattcc ccgccatggc agcgcgaccg cgagctcaca ggacttcatc gctgcggtcg
     7201 caccgaagtt ggcgatatta tcggcgggcg ccagaagccg tgccgagatg aagcgtgacg
     7261 aaatcgtcga gcgttatcat gccgccgggg ccgaggtgct gaagacctat gaggacggcg
     7321 cgattatttt ggagacgaat gggaataccc ttcgctatag cggtttcaag agcggcaaag
     7381 ccggtatgtt ggttttgtga accttgtcat cagaaaaaat ggcgcaaaga attcggagga
     7441 cgcaataata gaatcgggcg catcgtgaaa tttgcccgcc tcgcgcatat tatctggata
     7501 gtattacgaa aagcaggaac ggagtcggat gatgaagttg ggacgaaaac tttcgcccca
     7561 actaccaatc tccatgcctt cgggttgcaa gagtttctag agcgctttca gttcgccgat
     7621 atagtccttg aagccgttaa cgatgtcgta ctcgggagtc cagccgagaa tttgtttggc
     7681 tttctcgatc accataggtt gtttggccga gcgcggtttg attccgggaa tgatttccac
     7741 ttgtaagttc ggtagtaact tttcggcggt ggccaccaaa tcatcgaaag gaatgaccac
     7801 tccggtgccg atgttgaggg ttaccggttc tttcagtgga gtagttaccg ctttatccaa
     7861 ggcgcggccg agatctttgt agtagacata ttcgaaatcc cgggcgacct cctgggggac
     7921 cttgaccacg ccgcctttga tgcccgtttg caacatgttc tggaccatct caccgccgcc
     7981 ggagccgccg gcgaagtggc cgacaccgta aacgttagcc aatcgcaaga cgatcagctc
     8041 gaagccgtac atgcgctggt aggcctcgac catgagttcc ttggcgactt tggagttgcc
     8101 gtatgcttcg cccggaccgc ggcgaaaacc ttcgtcgatg ggatt
//
LOCUS       DASQKS010000344         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4375048, whole genome shotgun sequence.
ACCESSION   DASQKS010000344 DASQKS010000000
VERSION     DASQKS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4375048"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="VE170_08950"
     CDS             <1..1453
                     /locus_tag="VE170_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYJ15605.1"
                     /translation="SDAGSLYLVEEDGNGGRRLRFTLSQNDSGEPFRAESHLAIDNAS
                     VAGYVALTGVELAIDDVHRIDPTLPFRCNFTYGEESGHRIRSMLAVPMKNPQGEIIGV
                     VQLINCKGDAALPLDRHSLETMVVPFPEQCRPMVRSVASQAAVAIENIRLYESIETLF
                     EGFVRASVTAIESRDPTTSGHSFRVADLTLGLAQAVDRADTAPFRDVRFSRGEMKELR
                     YASLLHDFGKVGVREQVLVKAKKLYPGQLELIQQRFAYARKALEHAQSERKLAYLLDK
                     GRDEFLRQQTKFENEIEVQLQEVDELLGFIVRCNEPTFLHEGNVAGLARVAARQVADA
                     DGRPVPLLLPQEARLLAIPQGNLDDAERLQIQSHVDHTQNFLRQIPWTKEIKNVAEIA
                     AAHHEKLDGTGYPHSLAGRNIPIQSKMMTIADIFDALSAGDRPYKPAVPWERALDILH
                     DEGKRGMIDGDILRLFREAEVFRRVGQRRPDPV"
     gene            complement(1568..2731)
                     /locus_tag="VE170_08955"
     CDS             complement(1568..2731)
                     /locus_tag="VE170_08955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ15606.1"
                     /translation="MPGALDDIRVIDFTHALNGPFCTMLLGHLGAEIIKIEPPAGDGF
                     RRSWMPPDAKVDAYEFLWINVNKKSVVLNLKTAQGAGLAKQLIAKADVVVENYHTGQM
                     EKFGLGYEDLKKINPRIIYSCTRGFGETGPYKDYGSTAHTNNSMTGWTHTGWGYNKAP
                     GTKAIGIGDEAAGVSMVCGILAALHAREKSGEGQRIVVSMQEALLGFMISEFHEHFVG
                     IEVGNYPVPAADGYFTLRIPDISDRGWAKLAKLMDREDLLADPRFADAPARKQNRGQL
                     NDLIKAWAKTKTRQELWQGLRHIDYFGAPVLSMGEVIEDEHIKERKAFIDRNHPAAGP
                     TKLLAPWIHMSKTPASIRDDAPLLGQHTDDVLGNLLGLSAAQLSALRAENVIK"
     gene            2940..3932
                     /locus_tag="VE170_08960"
     CDS             2940..3932
                     /locus_tag="VE170_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15607.1"
                     /translation="MKNLMTKKFFFLGLCAMLLALSLSAQAQQPAGIPRIGILLPTTA
                     SVYLPRVEAFRRRLRELGYVEGKNTVIEYRYAEGTLERLPDLAAELVRLKVDVIVATA
                     QGALAAKKASATTPIVFGNASDPVGSGLVSSLARPGGNITGLSVMSADLDGKRLELLK
                     EAFPKVAWVAFLWEPGDSRGNRSLTDMEAAAKVLQLKLLSLEVRGLDDFDSAFARAKR
                     DGAQALITTTGPLVSTQLRQVLDFAAKNRLPAMYTNNEYVDAGGLMSYAPNQADLFRR
                     AADFVDKILKGTKPADIPVEQPKKFEFIVNLKTARQIGLTIPPNVLARADKVIR"
BASE COUNT          838 a         1114 c         1181 g          880 t
ORIGIN      
        1 gagcgatgcc gggtcgctct atctggtcga ggaggacggc aatggcgggc gccggctgcg
       61 ttttaccctg agccaaaacg acagcggtga accattccgt gccgagtctc atttggccat
      121 cgataatgcc agcgtcgccg gttatgtcgc gctcaccggc gtggagttgg ctatcgacga
      181 tgtgcatcgc attgatccga cattgccgtt tcgatgcaac tttacttatg gcgaggaaag
      241 cggtcaccgc attaggtcga tgctcgccgt accgatgaaa aatccccaag gagaaatcat
      301 cggcgtggtg caactgatca attgcaaagg tgatgcggcg ttgcccctgg accggcatag
      361 cctcgagacg atggtggtgc cgtttcccga gcaatgccgg ccgatggtcc gttccgtggc
      421 cagtcaggcc gcggtggcga tcgagaacat tcggctctac gagagcatag agacgctctt
      481 cgagggcttc gtgcgcgcgt cggtaaccgc catcgaatcg cgcgacccga ccacgtccgg
      541 ccattctttt cgcgtcgccg atttgaccct cggcctggcg caggccgtag accgcgccga
      601 caccgcgcca tttcgcgatg ttcgcttcag ccgcggcgag atgaaggagc tacgctatgc
      661 ctcgctgctg cacgacttcg gcaaggtcgg cgtgcgcgaa caggtgctgg tcaaagccaa
      721 aaagctctat cccgggcagc tcgaattgat ccaacaacgc tttgcctacg cacgcaaagc
      781 tttggaacac gcgcagagcg agcgcaaact ggcctacctg ctcgacaaag gacgggacga
      841 gttcctgcgc cagcagacga aatttgaaaa tgaaattgag gtccagctgc aggaagtcga
      901 cgagctgctc ggatttatcg ttcgttgcaa cgagccgaca ttcttgcatg aaggtaatgt
      961 tgccggcttg gcccgggtgg cggcacggca agtcgccgac gccgatgggc ggcccgtacc
     1021 cttattgctg cctcaggagg cgcggctgtt ggcgatccca cagggtaatc tcgacgacgc
     1081 ggaacgcttg cagattcaat cccatgtcga tcacacgcag aatttcttgc ggcaaatccc
     1141 ctggaccaag gagatcaaaa acgtcgcaga aatcgccgcg gcgcatcatg agaaactcga
     1201 tggcacgggt tacccgcaca gtttggcggg gcggaatatt ccgatacaat cgaaaatgat
     1261 gaccatcgcc gatatcttcg acgcactgtc ggctggcgac cggccctata agccggctgt
     1321 gccgtgggag cgggccctgg atattctcca cgacgaaggc aagcgcggca tgatcgacgg
     1381 cgatattctg cggctgtttc gtgaagccga agttttccgg cgcgtggggc agcggcggcc
     1441 cgacccggtg tagttgttca gctatcccgc cgcggtcgtg ttcgtgccgc gggcatgttt
     1501 gcgcccatcg tttgacgcaa cgcatagttt gcgcaggaac aattcgaatc ggcgattctt
     1561 gcgctgccta cttgatgaca ttctccgcgc gcagagcgct caattgggcg gcgctcaagc
     1621 ccaagagatt tcccaataca tcgtcggtgt gctggccgag taaaggggcg tcgtcgcgaa
     1681 tgctggcggg ggtcttcgac atgtgaatcc acggcgccaa gagtttcgtt ggcccggcgg
     1741 ccgggtgatt cctgtcgata aaggctttgc gttctttgat atgctcgtcc tcgatgactt
     1801 cacccatcga caacaccggc gcgccgaagt agtcgatgtg gcgcagtcct tgccaaagct
     1861 cctgtcgggt tttggttttc gcccaggctt taatcaggtc gttcagttgc ccacggtttt
     1921 gcttgcgcgc cggcgcgtcg gcgaagcgcg gatcggcgag caaatcttcg cgatccatta
     1981 atttcgcgag ttttgcccag cctctgtcgc tgatatccgg tatgcgcagc gtgaaataac
     2041 cgtccgccgc cggcaccgga tagttgccga cttcgatgcc gacgaaatgt tcgtgaaatt
     2101 cgctgatcat gaaaccgagt aaggcttcct gcatggagac gacgatcctc tgcccttcgc
     2161 cggacttctc gcgcgcatgc agcgctgcca gaatgccgca gaccatgctg acgccggcgg
     2221 cttcgtcgcc gatgccgatc gccttggtgc ccggtgcttt gttgtagccc cagccggtgt
     2281 gggtccagcc ggtcatgctg ttgttggtat gcgccgtgct gccgtagtct ttgtacggcc
     2341 cggtctcgcc gaagccgcgt gtgcaagagt agatgatacg cgggttgatc ttttttaaat
     2401 cttcataacc caggccaaat ttttccatct gcccggtgtg gtagttttcc acgacgacat
     2461 cggctttagc gatcaactgc ttcgctagcc cggcgccctg ggcggttttt aaattcagca
     2521 cgacgctctt cttgttgacg ttgatccaaa ggaattcata ggcgtcgact ttggcatcgg
     2581 gcggcatcca cgagcggcga aagccatcgc cggccggcgg ttcgatttta attatctccg
     2641 cgcccaggtg tccaagcagc atggtgcaga acgggccgtt caaagcgtgg gtaaaatcga
     2701 tcacgcgaat gtcatcaagt gcgccgggca tagggcctcc tggtttaagc aaggatgaaa
     2761 gcggcaggac gaaatcatcg ccgccgtcat ctcgtcttga aataatttgg ctggaacata
     2821 acacgaatcg gtgggcaggc aacagttttc accaccgcga attcgccatg ctcaccggag
     2881 gaaaaacttg acagccagat gacagcgtgg ctaccgttgt gcgatgttag cgatcgagag
     2941 tgaaaaacct catgactaag aaattcttct ttttaggtct gtgcgccatg ctccttgcgc
     3001 ttagcttatc tgcccaggcg cagcagccgg cgggaattcc ccggatagga attctgttgc
     3061 cgaccaccgc gtccgtctat ttgccccgag tcgaagcatt tcgccgacgg ctgcgcgagc
     3121 ttggctatgt cgaaggaaaa aacactgtca ttgagtaccg atacgcagaa gggacactcg
     3181 aacgactgcc cgaccttgca gctgaactgg tccgtctcaa agttgacgtc atcgtcgcca
     3241 ctgctcaggg tgctttagct gccaagaagg cgagcgcgac aacgcccatc gtgttcggca
     3301 atgcgagcga tccagtagga agtgggctcg tttccagctt ggcgcgacca ggagggaata
     3361 tcaccggact tagcgtcatg tcggcagatc tagatggcaa acgattggaa ttgctcaagg
     3421 aagccttccc caaggttgcc tgggtggcct tcctctggga accgggtgat tcgaggggaa
     3481 accggtccct cacagacatg gaggctgcgg ccaaggtgtt acaactaaaa cttctatcgc
     3541 ttgaggtgcg aggccttgac gattttgaca gcgcatttgc acgagcgaaa agggacggcg
     3601 ctcaggcgct cattacgacc acaggtccac ttgtcagtac tcagctacgt caagtgttgg
     3661 acttcgcggc taagaaccgg ttgccggcga tgtacacaaa caatgagtat gttgacgccg
     3721 gcggcttgat gagctacgcg ccgaaccaag cagacttatt ccggcgcgcc gctgactttg
     3781 tcgacaaaat attgaaaggc acaaagcccg ctgatattcc cgtcgagcag ccgaagaagt
     3841 ttgagttcat cgtcaatctc aaaaccgcca ggcagattgg tctgacgata ccgccgaatg
     3901 tgctggcgcg ggcggataag gtgatccggt gaattttgga tttagatttt cgattttggc
     3961 ttggggagaa aatgcacatg aataaaaagt cttggctcga agttctgtat cct
//
LOCUS       DASQKS010000345         6267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3548587, whole genome shotgun sequence.
ACCESSION   DASQKS010000345 DASQKS010000000
VERSION     DASQKS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548587"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VE170_08965"
     CDS             complement(<1..470)
                     /locus_tag="VE170_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15608.1"
                     /translation="MEDFAHADALFGLASSPEKISPVERLLNEFEAHEAKEDRALEVY
                     KGSIVHMPSPITRFLMQLIISDEEKHRALVHAMASTLRGSLNWTKPPDGLEGIADLST
                     MNGKVRAVTEELIHLEKDGIREYQTLVKESSGYYHGLFKVLLDSMIRDSEKHVE"
     gene            complement(530..1111)
                     /locus_tag="VE170_08970"
     CDS             complement(530..1111)
                     /locus_tag="VE170_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HYJ15609.1"
                     /translation="MSTEKVMIEEPRLAKMLFSDARFGLLWLPLRLYLGYMWFEAGWH
                     KLVDPKWMGSGEALLEYWKRGLKMAPKPVIAYDWYRGFIEYLISAEAHTWFSKIIIFG
                     ELVIATALILGAFVGVAAFFGGLMNWSFIMAGSASTNGMLFAIATWLVLAWRNAGWVG
                     LDRWLLPMLGTPWRPGRLFNANVAGRTANVRGR"
     gene            1450..1851
                     /locus_tag="VE170_08975"
     CDS             1450..1851
                     /locus_tag="VE170_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HYJ15610.1"
                     /translation="MPTNLAINGFGRIGRLVLRTVLERYKTDLNVIAINDMADLKTNA
                     HLFRYDSTYGMYSGQIETRDGAMMVDGREIRVLNHKDPSRLPWKQLGVDIVIEATGVF
                     NDAAQVRAHLNEWGYSCRVADLAMLMAEKGL"
     gene            1881..2252
                     /locus_tag="VE170_08980"
     CDS             1881..2252
                     /locus_tag="VE170_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ15611.1"
                     /translation="MTRDIITVSPSLKIKDLAMILTRNQISGAPVVDKQGKIVGIVSE
                     ADIIAKRGKETKAIMSKKVITVPLDASVEEIARLMAAHAITRLPVIDNGKIIGIVSRA
                     DIVQAIAMGEHTAIHAPVYDL"
     gene            2791..3027
                     /locus_tag="VE170_08985"
     CDS             2791..3027
                     /locus_tag="VE170_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15612.1"
                     /translation="MRAAQLLIRYIWLWDDERNPGKNDPSTSTYYTLPSEIRPGGSEP
                     FIFIPSPPPAQVTGGRYEVSVTVAGFTEVIPQPN"
     gene            3632..4048
                     /locus_tag="VE170_08990"
     CDS             3632..4048
                     /locus_tag="VE170_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ15613.1"
                     /translation="MKVVDLMTHDPLTVGTAETVGKADELMGENNIRQVPVVNGRELV
                     GIVTDRDVRAFLSDALLAKPEARERALKTSVGDIMTTEPLFISPDDDLKDAVEILIEQ
                     KFGAIPVVDAAEGLVGIVSYVDVLRLYLERLQEEGT"
     gene            4061..4210
                     /locus_tag="VE170_08995"
     CDS             4061..4210
                     /locus_tag="VE170_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ15614.1"
                     /translation="MTTTPDTSIKDLAHLMADKKIGGVPVVSDGMVVGLVTTTDILRY
                     VEGLA"
     gene            4270..4536
                     /locus_tag="VE170_09000"
     CDS             4270..4536
                     /locus_tag="VE170_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15615.1"
                     /translation="MGGFLSDQEVLTRERVKEILRNKEYSFFEEKVNLLEVRDDSLLL
                     KVSFLIEHKTRMIVIEEKDPIVVPFSDFKVEDWGDGVKKIADIK"
     gene            complement(4833..5261)
                     /locus_tag="VE170_09005"
     CDS             complement(4833..5261)
                     /locus_tag="VE170_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ15616.1"
                     /translation="MAMLKKILAPTDFSVLSGEGVRYACLLANEVGAQVIVLNVVVLD
                     ETNTVADGEIDAHKVQLTEFVAKALPHHAADVKRREVVLAGQPYSTIVDCAENERIDL
                     IVMSSHGRSGLSRMLIGSVTDRLLRAAKCPVMVVPSLGKH"
     gene            complement(5354..5449)
                     /locus_tag="VE170_09010"
                     /pseudo
     CDS             complement(5354..5449)
                     /locus_tag="VE170_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
     gene            complement(5498..>6267)
                     /locus_tag="VE170_09015"
     CDS             complement(5498..>6267)
                     /locus_tag="VE170_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ15617.1"
                     /translation="TLLMVADPNARPPFSMGQAAGDYLNHMAASLQPLSVQSVEKIGR
                     PAEVIVDSAKEGADCLIAMATHGVTGLRRWFMGSVASKVAQSAANPLLLIRPGEEGLP
                     PATLTLKRVVVPLDGSGLAEKVLPHVASLARKLKLEVLLVRVYELPLDAYVVANGMID
                     QGPAQFHRELRAECERYLDGKVAGLRADGYDSVSATILAGDAAHEIVDLTAATPQSLI
                     AMSTHGRSGVGRWVFGSVAEKIVQHSRAPVLLIRPSG"
BASE COUNT         1486 a         1708 c         1661 g         1412 t
ORIGIN      
        1 aactccacgt gcttttccga gtcgcggatc atgctgtcga gcaatacttt gaataagccg
       61 tgataatacc cgctactctc ttttactagt gtttgatact ctcgaatccc gtctttctcg
      121 aggtgaatca attcctctgt aacggcgcgg accttgccat tcatcgttga aagatcggcg
      181 atgccttcga gaccgtcagg cggttttgtc caattgagac ttcctcttaa tgtcgatgcc
      241 atcgcgtgaa ccaatgcgcg gtgcttctct tcatcagaaa taatcaactg catcaagaag
      301 cgcgtaattg ggcttggcat atgcacgatg ctgcctttat agacttcgag cgcacggtct
      361 tctttggctt cgtgagcttc aaactcgttt agcaatctct ccactggtga aattttttcc
      421 ggcgaagatg ccagaccgaa cagagcatct gcgtgtgcga aatcttccat ggctattgtc
      481 cttttttgaa tcggtaaaac cgttcatctt tctcgatgga ttcgtttact caccgtccac
      541 gaacgttcgc ggttcgcccg gcaacatttg cattaaacag ccggccgggc ctccaaggag
      601 tccccagcat cggcagcaac cagcgatcga gcccaaccca gccggcattg cgccatgcaa
      661 gcaccagcca agtagcaatc gcgaagagca tgccattagt cgaagcagat ccggccataa
      721 tgaagctcca gttcatcagc ccgccgaaga atgcggccac cccgacgaat gcgcccaaga
      781 tcaacgctgt ggcgatgacc aattcgccga agatgataat cttggaaaac caggtgtgag
      841 cttcggcgct gatgaggtat tcgataaagc cacgatacca gtcataggcg atcaccggct
      901 tgggcgccat cttgagaccg cgcttccagt actctagcag cgcctcgccg ctccccatcc
      961 atttcgggtc gacaagctta tgccatcctg cctcgaacca catatatccg aggtaaagac
     1021 gcaagggcag ccacagtaac ccaaaacgcg catcactaaa aagcatctta gccaacctcg
     1081 gttcctcgat catcactttt tctgtactca tgtttccccc tttgtgtttg ttctgatgta
     1141 aggatctcaa gcgcaaagca catgccacca aagaaagcgc caaatattta cgatccagcg
     1201 gcctgcgacc aattgttttc ttagttgtga gaattattta gccgggcttc gcaacagtgc
     1261 aaattgaaat agcggttcgg aacgctttgc tgcgataata tctaggttta cgagcggcac
     1321 gaaaatcgct gtgagtttag ctggtgccaa gggttagcgg ataaacaata tcgttcagca
     1381 gagcaacttc gcgatcgttt tcaatatcca aaaacaaccc tgcgcagctg tagttgagag
     1441 gatcccatca tgccaaccaa tcttgcgatc aacggttttg gtcgaatcgg ccgactggtg
     1501 ctacgcaccg ttctggaacg ttacaagaca gatctcaatg tcatcgctat caacgacatg
     1561 gccgacttga aaaccaatgc ccatttgttt cgctacgact ccacttatgg aatgtattct
     1621 ggacaaattg aaactcgcga cggcgcaatg atggtcgacg ggcgggaaat ccgcgtactc
     1681 aaccataagg acccttcccg tttgccctgg aaacagttgg gggtcgacat cgtcattgaa
     1741 gcgaccggcg tttttaacga cgccgcccag gtgcgcgccc atctcaacga atggggttac
     1801 agctgccgcg tcgccgatct cgcaatgtta atggcagaga agggacttta ggaggaaaca
     1861 atatgatagc tagggacgtt atgactcgcg acattattac tgttagccca agcctcaaaa
     1921 tcaaagattt ggctatgatc ctaaccagga atcagatcag cggcgccccc gtcgtagaca
     1981 aacaggggaa gattgtaggc attgtttcgg aagcggatat cattgccaaa agaggcaagg
     2041 agaccaaggc gatcatgagc aaaaaggtga tcaccgttcc cttggatgca tcggtggaag
     2101 agatcgcccg actcatggcg gcccatgcca tcacgcgctt gccggttata gacaacggaa
     2161 aaataatcgg aatcgtcagc cgcgccgaca tcgtccaagc tatcgccatg ggtgagcaca
     2221 ctgcaatcca tgctcccgtc tatgatctgt agcagtttca gatgcgcacc acgtcgccgt
     2281 gtagcgagaa cttgcccgcc aatcacgccg ccagccgatg aatttttcga aagccattct
     2341 ggaacatatt tctcaaagat acccgggtac catatcggtt ttgcccgtgt tgcaggtcta
     2401 ttggagcgat ttgggctcgc ctcaacggct gttggagagc caacggctct tcggccctcg
     2461 ccgaccgcgc gcacctacag cccgatcacc gcttaatctc gatgacagcg aactggttcg
     2521 aatagccggc actggcatga tattgggaga tccaagaata gggatctgag cacaaggagc
     2581 tatttgccta tgtcacagga gaaatgccga cactggcgaa cgattaccgc ggcactcttt
     2641 actggcatcg tcgttctcgc agcaccatat gcgggttggg cgcagaaaat agcttcgccc
     2701 gagagtatac gggggatcct gacggtcgag aaggtctctg tctcagacgg cagggtatcg
     2761 ggtgaagttc gtagtcgagg gccctatgtc gtgcgcgctg cgcagctgtt aatccgttac
     2821 atttggctgt gggatgatga acgcaaccca ggcaaaaacg atcccagcac atcgacttat
     2881 tatactttac cgtcggaaat ccggccggga ggaagcgagc cgtttatctt catcccctcg
     2941 cctccgcccg cgcaggttac cgggggccgc tacgaagttt cagtaaccgt ggccggcttt
     3001 accgaagtca ttccgcaacc gaattgatac ctttcacgac ctggcaggca actcagaatg
     3061 actgcaatcc gtgccgccaa attacgtaat attttcggta cgcgcaggat ctccggccgg
     3121 tcgggagttg cgcccgccgt gctagctcac gtgtccgcat ccttgcaact gctcatcgcc
     3181 aataaatcac cggcgcctag atgatggcgt cacaaagggc gctgactgaa tcgcttgagc
     3241 aatgccacta tcgctcttgg tcgacttcgg cacctatgac gagtgcgacc gggtacgcca
     3301 tagaattctt tgacgatcat gctcgggcga taggtcaaga aacgtaagct ccctgtagag
     3361 tctctaagat accgcaaaga agagctgcgc ctacaggcat cggcgggatt gagcctgggg
     3421 tcgctgctgt cagtaaaacc aaatttgcgg tatttacctt gagttttttc gcggcgctgc
     3481 gcaaatcgat ggcgatattc tcatttgtga aaaccaactg ccgattcagc gccgcaaccg
     3541 cgccaactct cacggtaatt tattttgcaa gtttggcacg gacactgcac cgataactaa
     3601 tagaagcagc actcttaagg aggaatcttg gatgaaagtt gtagacctga tgacccacga
     3661 cccattgacc gtagggaccg cggaaaccgt cggtaaagcc gacgaactca tgggggagaa
     3721 caatatccgg caagtgccgg tggtcaacgg ccgtgaactc gtcggcatag tcaccgaccg
     3781 cgatgtccgg gcgtttctca gcgacgccct attggccaaa cccgaagcca gagaacgcgc
     3841 cttgaagaca tcggtgggcg atatcatgac cacggagccg ctgttcatca gccccgatga
     3901 cgatctcaaa gacgcggttg aaatactcat cgaacaaaaa ttcggcgcca ttccggtggt
     3961 cgacgcagcc gagggactgg ttggtatcgt ttcctatgtc gatgtgctgc gcctttatct
     4021 cgagcggcta caggaggaag ggacatgaaa gaagccggtc atcaccacaa cgcccgacac
     4081 gtcgatcaaa gatcttgcgc atttgatggc cgataaaaaa attggcggcg tgccggtggt
     4141 cagcgacggt atggtcgtgg gcctagtgac gacaaccgac attttacgtt acgtcgaggg
     4201 tctggcatga ccggcatatg caatcggaaa ttttactttg tcataggttt cgcacactaa
     4261 ggaggtatta tgggcgggtt tttaagcgac caagaagtgc tcactagaga acgtgtcaag
     4321 gagatactgc gcaataagga atacagcttc ttcgaagaga aagttaatct gctcgaagtc
     4381 agggacgatt ccttgttgct gaaagtttcc tttctgatcg aacacaagac acgcatgatc
     4441 gtgatcgaag aaaaagatcc cattgtcgtg ccgttcagcg atttcaaagt cgaggattgg
     4501 ggcgacggcg tgaaaaaaat cgccgatatc aaatgagcga ttctggttcg ctgccttaga
     4561 agattgctag tgagtctgcc ggcgctgcgg cacattgcgc ggggctcatc gagcgcccct
     4621 cccccccggc ggcaagagcg tgtcggcaac cgttcttcaa gacaatcgac ttcgtcttcg
     4681 atcacatcca ggattggcaa gtcagtgtca atgatcaggc aaccgcggtg ctgcgacggc
     4741 acttattcca atcggcaggt caatatgctt tgcgctcatt catttcgtcg gctcatcgtt
     4801 tcgcgaatgc aacgggccaa atcactcgcg ctttagtgct tgccaagcga tggcaccacc
     4861 attaccggac atttcgccgc gcgcagcaac ctgtccgtga cgctgccgat cagcatgcgc
     4921 gacaggccgc tgcgtccatg ggacgacatg acgatcagat cgattcgctc gttttcggcg
     4981 caatcgacga tagtgctgta gggctggccg gcgaggacca cttcacgacg tttcacgtcg
     5041 gcagcgtgat ggggaagagc cttcgcgaca aactctgtga gctgcacctt gtgtgcgtcg
     5101 atctcgccat cggcgacggt attggtttca tcgaggacaa cgacattcag gacgatgacc
     5161 tgagccccga cctcattggc caaaaggcag gcatagcgaa ccccttcgcc cgagagtacg
     5221 gagaaatcgg tgggcgcgag aatctttttt agcatggcca taacgaacct ctttccacgg
     5281 taggattttt tcgctcaagc agtaattagt taacgcaacc aagatgccag gctgtgaatc
     5341 atgatttgtg cacttcatgc atatcttcaa ggctgttgat gaagtggctc tgaccgagga
     5401 taaatttgat ttcgtcgggc ttgtcgatgg caacggcggt caattccata atgcgataac
     5461 ctgggagcag cgcgcgtgaa gatttactcg ctcaatccta gccgctaggg cgaatcagca
     5521 gcaccggcgc gcgcgagtgt tggacgatct tttcggcgac actgccgaac acccaacggc
     5581 cgacgccgga cctgccgtgg gtcgacatgg cgatgaggct ttgcggcgtc gctgctgtca
     5641 ggtcgacgat ctcgtgggcc gcgtcgcccg cgaggatcgt ggcgctcact gagtcgtagc
     5701 cgtcagcccg cagcccggca actttgccgt ccaaatatct ttcgcattcc gcacgcaatt
     5761 ccctatgaaa ctgcgccggc ccttgatcga tcatgccatt ggcgaccaca taggcatcga
     5821 gcggcaattc atagactctg accagcaaca cttcgagttt cagttttctt gccagggacg
     5881 cgacatgggg cagaactttc tcggccaagc ccgagccgtc cagcggaact acaacccgct
     5941 tgagcgtgag ggtcgccggt ggcagacctt cttctcccgg gcggatcagc aagagtggat
     6001 tggcggcgga ttgcgctact ttgctggcga cactgcccat gaaccagcgc cggagaccgg
     6061 ttacaccatg ggttgccatg gcgattagac aatcggcacc ttcctttgcg ctatcgacga
     6121 tgacttcggc gggtctaccg atcttctcca cggattggac cgatagcggc tgcagacttg
     6181 cagccatgtg attgagatag tcgccagcgg cttgacccat ggagaagggc gggcgcgcgt
     6241 tgggatcggc gaccattagc aatgtca
//
LOCUS       DASQKS010000346         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1444559, whole genome shotgun sequence.
ACCESSION   DASQKS010000346 DASQKS010000000
VERSION     DASQKS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444559"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1372
                     /locus_tag="VE170_09020"
     CDS             152..1372
                     /locus_tag="VE170_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15618.1"
                     /translation="MNRRQFNKTLLTAGAAVLAPFNIARAQSNKLKIGVLLPKSGLQG
                     LIGQSCQKGADLAPGIIRELLGVDIELLNADTESNVDIARTRAEKLIQDGAHCLVGPF
                     DSGAAAAIAQVAEQRGVPFIINIAAAPQITEQGYKFVFRNFPTATELVRNGLALTDEL
                     FKATGTTPRTAVFMHVNDTFGQANAKAIGAILPQLQLPFKLVETISYDPAAKDMTVEV
                     AKAKATKADFLLLVCRLNDAIVLRREIVKQRWNVMGIVSPGSPGMYENQFFQTLGKLS
                     EGCISNVPWFDPKAAMAKSVDAAFRKQNPKDQLKFHALNVGYTFEALLIAADAFKRAK
                     SPDAQPLTDAIRQTEIKHRMMIGAPIKFNAKGQVEGNLSACIQNQRQQPVVVLPAPSA
                     EAKPVFPWREYKRG"
     gene            1483..2358
                     /locus_tag="VE170_09025"
     CDS             1483..2358
                     /locus_tag="VE170_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYJ15619.1"
                     /translation="MTIDFITCANVIISGTLTGLVYGLMALGLSVIFGVARVVNFAHG
                     EMMTLAMYASVILFGAFELNPFIAVVPVAASFFLLGYGLQRWIVNPFVTRPEHSQFML
                     LLAIAIILTNIQLMFFGPDARSVRLDSLLESIELGSLLIDRGKLYAALVALGTAMALL
                     SFFRFTRSGTAIRACADNWLGAKVVGLNVKQLYALAFGLGSVCVAVAGCMMILLVDLT
                     PDIGPGYTMLAFVIVIVGGLGSMNGALVGGVLIGLSEALAGLFILPSAKSMFSFALLV
                     IVLILRPRGLLGGRR"
     gene            2373..3326
                     /locus_tag="VE170_09030"
     CDS             2373..3326
                     /locus_tag="VE170_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYJ15620.1"
                     /translation="MLLALSAIALIALPAFANNYLISVATLCLFFAYTGQAWNIMMGF
                     AGQLSLGHALYLGVGGYSAAALYFHYGIGPWLGVGLAIILSIALGAFLCALAFRYGIS
                     GVYFTLLTIAFAEFARIGFDHLAWTGGSAGLFLKVAQRDRADLVNLRGPPAMYYYVIL
                     ALTALACGFSAWLLRRRAGYYWQAIRENEEAARALGINTIRWKILAVIISAAMTAVAG
                     VFIAFYYNNLFPEQIFNINRSVEIILGPIVGGMGTLFGPIIGAALMTLLTDGATELVA
                     ALGWNIPGVKQLIYGLVLLAVVVFLPEGVWPAMARKLKLDR"
     gene            3323..>3556
                     /locus_tag="VE170_09035"
     CDS             3323..>3556
                     /locus_tag="VE170_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ15621.1"
                     /translation="MSLLEVRNVDKSFSGLRAVRAVSFSVAANSITALIGPNGAGKTT
                     LFNLIAGALAPDSGTIMFLDRRSDGLRPDQICAR"
BASE COUNT          682 a         1093 c          970 g          811 t
ORIGIN      
        1 tccgacttga attgcgacct tacggaccag gacactaggg tttcggttat tagtgatgaa
       61 ctaattagcc caccgtcaaa gtggttaacg acgaaatctg atcatgctat acaaacgaac
      121 tattatccga gacaagccta cgggaaagaa aatgaaccgc cgacaattta acaaaacact
      181 gctcaccgcc ggtgctgccg tactcgcgcc gttcaacatc gcccgcgcgc agtccaacaa
      241 actcaagatc ggcgtgctgc tgcccaagtc cggcctgcag ggactgatcg gccagtcctg
      301 tcagaaaggc gccgatctcg cgccgggaat tattagagag ttgctcggcg tcgatattga
      361 attgcttaac gccgacaccg aaagcaacgt cgacatagcg cgcaccaggg cggaaaaatt
      421 gattcaggac ggcgcccatt gtctggtcgg gccgttcgat tccggcgccg ccgccgccat
      481 cgcccaggtc gccgagcagc gcggcgtgcc gttcatcatc aatattgcgg cggcgccgca
      541 gatcactgag cagggctaca aatttgtttt tcgcaacttc cccaccgcca ccgagttagt
      601 caggaacggc ttggcgctca ccgacgagct gttcaaagcc accggcacga cgccgcgcac
      661 cgcggtgttc atgcatgtca acgacacctt cggtcaggcc aacgccaaag cgatcggcgc
      721 gattctgccg caactgcagc tgccgtttaa gcttgtcgaa accatttctt acgatccggc
      781 ggcgaaggac atgacggtgg aagtcgccaa ggcgaaggcg accaaagctg attttcttct
      841 gttggtctgc cgtctcaacg atgccatcgt gctgcgccgc gagatcgtca aacagcgctg
      901 gaatgtgatg ggcatcgtca gccccggctc ccccggcatg tacgagaacc aatttttcca
      961 aactctcggc aaattgtccg aaggctgtat ttccaacgtg ccctggttcg accccaaagc
     1021 cgccatggcg aaatccgttg acgcggcgtt tcgaaaacag aatccgaaag accagttgaa
     1081 atttcatgcg ctcaacgtcg ggtatacctt tgaagccttg ctgatcgccg ccgacgcctt
     1141 caagcgcgcg aagtcgcccg acgcccagcc gctcaccgac gcgattcgcc agactgaaat
     1201 taagcaccgc atgatgatcg gcgcgccgat caagttcaat gccaaaggcc aagtcgaagg
     1261 caacctttcg gcctgcattc agaatcaacg tcagcagccc gtggtcgtat tgcccgcccc
     1321 atccgctgaa gccaagccgg tgtttccttg gcgcgaatac aagcgcgggt aacgcgacct
     1381 aaggtcgcgg ttcaaggttc aaaagttcaa tgttcaacgt cagatccgaa ccttggaccc
     1441 ttgaaccctt gtacccttga accttgaacg gaagttttga agatgaccat cgacttcatc
     1501 acctgcgcca atgtcatcat ctccggcacc ctcaccggcc tggtctatgg cttaatggcc
     1561 ctgggcctct cggtcatctt cggcgtcgcg cgcgtggtca acttcgccca tggcgagatg
     1621 atgacgttgg ccatgtacgc cagcgtgatt ttgttcggcg cctttgagct gaatccattc
     1681 atcgccgttg ttcccgtcgc cgcgagcttc ttcctcttgg gctacggctt gcaacgttgg
     1741 atcgtcaatc ccttcgtcac ccgtccggag cacagccaat tcatgctgct gctcgcgatc
     1801 gcgatcatct tgactaacat ccaactgatg ttcttcggcc ccgacgcgcg cagcgtgaga
     1861 ctcgacagct tgcttgaatc gatcgagctc ggctcgctgt tgatcgaccg tggcaaactt
     1921 tacgccgcgc tagtcgctct aggcacggcg atggcgctgc tcagcttttt ccgcttcacc
     1981 cgcagcggca ccgcgatccg cgcctgcgcc gacaactggc tcggcgccaa agtcgtcggc
     2041 ttgaacgtca aacagctcta cgcattggcg tttggtttag gctctgtctg tgtcgccgtc
     2101 gccggttgca tgatgatctt gcttgtcgac ctgacgccgg acatcggccc aggctatacg
     2161 atgctcgcct tcgtcatcgt catcgtcggc ggattgggtt cgatgaacgg cgcgctggtt
     2221 ggcggcgtgc tgatcggctt gtcggaagcg ctggccggat tattcatcct gccgtcagca
     2281 aagtccatgt ttagctttgc cttgctggtc atcgtgctga tcctgcgacc gcgcggcctg
     2341 ctaggcggcc ggcggtgaac cggcgtgggg cattgttact ggcattatcg gcgattgcgt
     2401 tgatagcgtt gccggccttt gccaacaact atttaatatc agttgccacg ctctgcttgt
     2461 tcttcgccta caccggtcaa gcttggaaca tcatgatggg tttcgccgga cagctctccc
     2521 tgggccacgc tttgtacctt ggtgtcggcg gttacagcgc ggcggcgctt tattttcatt
     2581 atggcatcgg tccctggctg ggcgtggggc tagcgatcat tttgtccatc gcgctcggcg
     2641 catttctctg tgccctggcc tttcgctacg gcatctccgg cgtttacttc acgctcttaa
     2701 ccatcgcctt cgctgaattc gcgcgcatcg gcttcgatca tctcgcttgg accggcggtt
     2761 cggcggggct atttctcaaa gtcgcccagc gcgaccgcgc cgatctggtc aatctgcgcg
     2821 gtccgccggc aatgtattac tacgtgattt tggcgctcac cgcgctggca tgcggattct
     2881 cggcgtggtt gttgcgtcgc cgggccggtt actactggca agcgattcgg gaaaacgagg
     2941 aagcggcacg cgctctcggc atcaacacaa tccgctggaa aatactcgcc gttattatca
     3001 gcgccgccat gaccgcggtg gcgggggtgt ttattgcgtt ctattacaat aatctttttc
     3061 ccgagcagat tttcaacatc aaccgctccg ttgaaatcat cctgggtccg atcgtcggcg
     3121 gcatgggcac cctgttcggc ccgataattg gggccgcgct aatgaccttg ctcaccgacg
     3181 gcgccaccga attggtcgct gccctaggct ggaatatccc cggtgtcaaa caactcatct
     3241 atggtctagt tctattggcc gttgtcgtgt ttctacccga gggcgtctgg ccggcgatgg
     3301 cgcgcaagtt aaagttggat cgatgagctt actcgaagtc cgaaacgtcg acaaatcctt
     3361 cagcggtctg cgcgccgtgc gcgctgtttc gttcagcgtg gccgcgaatt ccatcaccgc
     3421 cctcatcggt cccaacggcg ccggcaagac caccctattc aatctcatcg ccggcgcgct
     3481 cgcgccggac tcagggacga tcatgttcct ggacaggcga agcgacggcc tgagacccga
     3541 tcaaatctgc gcccgc
//
LOCUS       DASQKS010000347         4235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3122479, whole genome shotgun sequence.
ACCESSION   DASQKS010000347 DASQKS010000000
VERSION     DASQKS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3122479"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(491..1654)
                     /locus_tag="VE170_09040"
     CDS             complement(491..1654)
                     /locus_tag="VE170_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15622.1"
                     /translation="MAETNLQLFLLGAERLLRCRWDGRSENLEILNSAMDGETVREVA
                     RDPFNPKKLYAATLTEIHVSENGGESWQWLPSGGIDYRDIWTMAVHPTRPDEIYVGTL
                     PAAVYVSDNGGRSFRELAAFRNLPDYNKWTFPPGAHVAHARSIALDARVPDEIIVGIE
                     EGGVARSRDRGVSWEDISGPPSPTAFPKVNDAAGIVPYEMGKHEEGRVYRDVHLVTRH
                     PARLATLFASTGIGTYRTDDGGNNWTKCEYGLGRAYAIPMTIHPAVPERIFLGAAENG
                     PATWAGYRTVRAGPYNTVKFSRNATAETGGAKTQILRSDDAGKTWNFLKTGLPAGTEH
                     MTCGFASHPQDPDTLCVGYTDGSIYASNDAGLNWRRLIVSLPKLYGLRLIPAV"
     gene            1800..2675
                     /locus_tag="VE170_09045"
     CDS             1800..2675
                     /locus_tag="VE170_09045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15623.1"
                     /translation="MRPVKPPNPNPHPPRYKLPAGACDCHLHVYGPFERYPLIAERGY
                     DPDPHSTLDDYLQTHRALGLERAVIVTGSGNGTNNRITLDALKRMNGTFKGLALLDPA
                     VTDAELAELKDGGFSGFRIKANGRGGLSFDETKRMIARTRGFNWHVEFMSQSLAEVIA
                     AVPFLDSLKVPYMFDHVAHAEPHMTGDNSEFVELLRILKNAEHAWLNLYSFYQLSETG
                     PADYRDMVGVVQTLVANRSDNIVWGSNWPHGGVSVPMPDDGDLLDFLCTAVPDEKIRD
                     AILAANPAKLYGWPA"
     gene            2783..3508
                     /locus_tag="VE170_09050"
     CDS             2783..3508
                     /locus_tag="VE170_09050"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15624.1"
                     /translation="MYGWRARIGHVAPSRGDTLVYEFYKMFPEGFMMLNTTGTVRQLV
                     DSDFEAQLSRIEEATQDLVDNNCDSIIFSGSPLFTRLPFGADRELGKKLSDKFGVPVA
                     AGLTAEVEALKAMHCTKLVVGTPYEDEINQRLKRHLEQCGFEVLQIAGYGVRKNAQLT
                     DLPVHASYKIAKRLYAQARDADGVFIPCPRWPTITDVQLLEDEIGKPVITSSLACSWY
                     AMRLIDIREKVKGFGRLMESLAP"
     gene            3590..4066
                     /locus_tag="VE170_09055"
     CDS             3590..4066
                     /locus_tag="VE170_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15625.1"
                     /translation="MSWIKAAFLTGLLSSLLINATPQAMAADGDRRGGRGCQRGDRIN
                     IQDLDMSPDPVIEGQRVRAWKVRINFEGGRECETDIVVREGNNVVGQSRNFRMRPGVN
                     ELEIPAADNFRHSGREHCFNVQVDLDGSRQQVDAARRFCARQRPMWSMREAGDRGR"
BASE COUNT          918 a         1289 c         1139 g          889 t
ORIGIN      
        1 gcattggcct tgagccaatc agcgttgctt tttaacgttg cccgggcggc ttcagaaagc
       61 gccgcgctgt cgaagttgaa gccgacatct ttgagcggtc cgctggtcgg agcctcgccc
      121 ttttgcagcg cctctaaact cgtcttctct tccgctttcg acgtcacggg cgggggtttc
      181 ggcgccgccg gttttgctgc ctcagtcgaa ggattctccg ccgtccattt cggcggcggc
      241 ggatttgcgg gattaccgca tgaaactaaa aaaataaccg ccagcaattt caatccacaa
      301 tgcaaagtcc gcttttgctc caccatcgaa accacctttc cagccttcgt tctgactgat
      361 taattggtta attgtgaagt atcgtacgga acctgtcaac catttcagtc tacaaaacaa
      421 aattttataa aatcgccgat tcaagccaca aaaacaatga aaccagggtt tttttacggt
      481 taagacgccg ctaaacagcc ggtatcaatc gtaaaccata aagctttggc agcgacacga
      541 ttaatctgcg ccaatttaat cccgcgtcat tgctcgcgta gatcgaacca tcggtatagc
      601 cgacgcataa agtgtcgggg tcctgcgggt gactggcaaa gccacaggtc atatgttcgg
      661 tgccggcagg taggccggtt ttaaggaagt tccaggtttt gccagcgtca tcgctgcgta
      721 aaatttgcgt cttggcgccg ccggtttcag cggtcgcgtt gcggctaaat tttaccgtat
      781 tgtacgggcc ggcacggacc gtccgatatc ccgcccaggt cgccggaccg ttctccgccg
      841 cgccgagaaa aattctctct gggaccgctg gatggatggt catgggaatc gcgtaggcgc
      901 gccctaggcc atattcgcat ttggtccagt tatttccgcc atcgtcggtg cgataagtgc
      961 cgatcccggt gctagcgaat aacgtcgcca agcgcgccgg atgacgcgtt accaagtgaa
     1021 catcgcggta aacgcggccc tcttcgtgtt tgcccatttc ataaggcaca atcccggctg
     1081 catcgttgac cttgggaaat gccgtcggac tcggcggtcc gctgatatct tcccagctca
     1141 cgccgcggtc acggctgcgc gccacgccgc cttcttcgat accgacaatg atctcgtccg
     1201 gcacccgcgc atcgagcgcg atcgagcgcg cgtgggcgac atgggcgccg ggagggaacg
     1261 tccatttgtt gtagtcgggc aaattgcgaa acgccgccag ttcgcgaaac gaccggccgc
     1321 cgttgtcgct gacataaacc gccgccggca gcgtaccgac atagatttca tcgggccggg
     1381 tcggatgaac cgccatggtc caaatatccc ggtagtcgat accgccggag ggaagccact
     1441 gccagctctc gccgccattt tcgctgacat gaatctcggt cagcgtcgcg gcataaagtt
     1501 ttttcggatt gaacggatcg cgcgccacct cgcgcacggt ctcgccgtcc atggcgctat
     1561 tgagaatctc gaggttttcg ctccggccgt cccagcggca acgcagcagt cgttccgcgc
     1621 ccaacaagaa cagttgcagg ttcgtttctg ccataactta cctccgccga gtcttatgat
     1681 tggtctgtag tgatcagcaa tacgctaagc aaaacctccc gcgccgtcaa gcgataatcc
     1741 gccaaggcta gaaatgcgct ggatattcat cccgcttttg cattacattg gcgctcatca
     1801 tgcgaccggt aaagccgccc aaccccaatc cccacccacc gcgctataaa ttgcctgccg
     1861 gcgcttgcga ctgccatctg catgtttatg gtccgttcga gcgttacccg ctgatcgccg
     1921 agcgcggcta cgatcccgat ccgcactcga cgctcgacga ttatttgcaa actcatcgcg
     1981 ctctcggctt ggaacgcgcc gtcatcgtta ccggcagcgg caatggcacc aacaatcgca
     2041 tcaccctgga cgcgctcaag cgcatgaacg ggaccttcaa aggcctcgcg ctactcgacc
     2101 cggcagtcac cgatgcagag ctagccgaac tgaaagacgg cggcttcagc ggctttcgga
     2161 ttaaagccaa cggccgcggc gggctttcct tcgacgaaac caagcggatg atcgcgcgca
     2221 ctcggggatt caactggcac gtcgagttca tgtcgcaatc gctggcggag gtgatcgccg
     2281 ccgtgccgtt tttagattca ctcaaagtcc catacatgtt cgaccatgtc gcccacgccg
     2341 agccgcacat gaccggcgat aatagtgaat ttgtcgagtt gctgcgtatt ctaaaaaacg
     2401 ccgagcatgc gtggctcaat ctttacagct tctaccagct ttcagaaacc ggccccgccg
     2461 attaccggga catggtcggt gtcgtgcaaa cgctcgtcgc caaccggtcc gacaacatcg
     2521 tctggggcag caattggccc catggcggcg tgtctgtgcc aatgcccgac gacggcgacc
     2581 tgctcgattt cctctgcacc gccgtgcccg acgaaaaaat ccgcgatgcc attctcgccg
     2641 ccaacccggc aaagctctac ggttggcccg catagaccca tggacgatcg gaacaattgg
     2701 aactgctgga agcgagtata gcaaatccga ccttgaattt ttgaaccctt gaaccctgaa
     2761 caactccgca ggaggtaaaa atatgtacgg ttggcgcgct cgcataggtc atgtcgcccc
     2821 atcgcgcggc gacaccctgg tttacgaatt ctacaagatg tttcccgaag gcttcatgat
     2881 gctcaacacc accggtacgg tgcgccagct ggtcgacagc gacttcgagg cgcagctgag
     2941 ccgcatcgaa gaagcgaccc aggacttggt cgacaacaac tgcgactcga taatctttag
     3001 cggctcgccg ctgttcaccc gcttaccttt tggcgccgac cgggagctgg gtaaaaagct
     3061 ttcggataaa ttcggcgttc ccgtggccgc cggtttgacc gctgaagtcg aagcactcaa
     3121 agccatgcac tgcacgaaac ttgtggtcgg tacaccctac gaagacgaga tcaatcagcg
     3181 actcaagcgc catctcgagc agtgcggctt cgaagtctta cagatcgccg gatacggcgt
     3241 gcgcaaaaac gcccagctca ccgacctgcc ggtacacgcc tcgtacaaga tcgccaagcg
     3301 gctctacgcc caagcgcgcg acgccgatgg ggttttcatc ccctgcccgc gctggcctac
     3361 catcaccgac gtccagctcc tcgaagacga aatcggcaag ccggtcatca ccagcagcct
     3421 ggcctgtagc tggtatgcca tgagattgat cgacatcagg gaaaaagtga aaggttttgg
     3481 ccgcttgatg gaaagtttgg caccttaggg gtcgagctca attttgctgt agccattttt
     3541 cgtttcctac gtctaaactg atagacaaaa ccttgaagga gaaaaacata tgtcgtggat
     3601 caaagcagca tttctaaccg gtttgctcag cagcctactg ataaacgcta cgccgcaagc
     3661 catggcggcc gacggagacc gccggggcgg ccgcggttgc cagcgcggcg atcgcataaa
     3721 tattcaggat ctcgatatgt cgccggatcc ggttatcgag ggccagcggg ttcgcgcctg
     3781 gaaggtacgc atcaatttcg agggcgggcg cgaatgcgaa accgatattg tcgtccgcga
     3841 gggtaacaac gtcgtcggtc aatcccggaa tttcagaatg cgccctggcg tgaatgagct
     3901 cgagattccc gccgctgata acttccgcca cagcggccgt gaacactgtt tcaatgttca
     3961 ggtagacctc gacggctcgc gccagcaggt cgacgccgcg cgccgtttct gcgctcgcca
     4021 gcgtcccatg tggagcatgc gcgaagccgg cgatcgcgga cgctaatatt catagctaaa
     4081 gcaccccggg ggaaaaaaga cttgggacag agagccgcgc tcgaacggtt ctctgtcccg
     4141 gtcttttcac cgccttcgca acaccaccag cgccgtaccg gcgagaatca atccgcaccc
     4201 tgccaccatc aaggcggtga cagtttcgcc gagaa
//
LOCUS       DASQKS010000348         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1038623, whole genome shotgun sequence.
ACCESSION   DASQKS010000348 DASQKS010000000
VERSION     DASQKS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038623"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VE170_09060"
     CDS             complement(<1..909)
                     /locus_tag="VE170_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15626.1"
                     /translation="MKLESRVAVIVSAAMLAVIFAVEFANAQANPDPRLIEGAKKEGE
                     VIFYTTMTLDQSKEVVDRFQKKYPFIKPTLFRTGGGPLLNKILNEARAGRHAWDVVAG
                     RAEMYVPLMERKLLASYRSPEAKAIDEDLVDKEGYWTAYYVVTYVLGWHTKLVKREDV
                     PKTYEALIESRWKGQLSFDNEAYGMLQGLMRAWGREKAIAYFRRIAAIDPAMMRGNSE
                     RVALAAAGEYPLIVAYNQTLERMVARRAPLDWLPLEPAVVSAFPIMIAAKAPNPNAAR
                     LYYDFSLSKEGQEMMRGMQRIPVRKDV"
     gene            complement(945..1955)
                     /locus_tag="VE170_09065"
     CDS             complement(945..1955)
                     /locus_tag="VE170_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15627.1"
                     /translation="MEQLTLGFISAFNERVEPLMNGTIEVEGVELIPTYSHPAETFWR
                     QLKFGEFEVAEMSLSSFLIARSQGSDMIALPAFPSRRLFQTELSYHVDSGIEKPQDLV
                     GKRLGVAEYQQTAALWLRGILEHDFGVSQYRIHWYMERSEEMSHGGATGFKPPPGISF
                     QRIAGNKSLASTILANELDVAHVSSPWVLQANTLDRSSRIAGKGDWSQIKPLFPDRMA
                     EGARFYRQHGFLPVNHAYIIRGDIHRKHPWLAGKLYDGFVRAKAYARDRLAAQIPTAL
                     IFGQEYLETTRELIGDDPFPFGIAANRAMLDTITSYSREQGLTPRQMKIEELFAESTL
                     DT"
BASE COUNT          441 a          632 c          646 g          508 t
ORIGIN      
        1 gacatctttg cgcaccggaa tacgctgcat gccgcgcatc atctcctgtc cctctttgga
       61 caggctgaaa tcatagtaga gccgggcggc gttgggattg ggcgccttgg ccgcgatcat
      121 gataggaaag gcgctgacga ccgccggctc caaaggcaac cagtcgaggg gcgcgcgcct
      181 ggcaaccatg cgttccaagg tctgattgta ggcgacgatt agcggatact cacccgccgc
      241 cgccagtgcg actctttccg agttgccgcg catcatggcg ggatcgatgg ccgcgatgcg
      301 gcgaaaatag gcgatggctt tttcccgtcc ccaggcgcgc atcagccctt gcagcatgcc
      361 gtacgcctcg ttatcgaaag acagctggcc tttccatctt gactcgatta gggcctcgta
      421 ggtcttcggc acatcctcgc gcttgaccag cttggtatgc catcccagga cgtaagtgac
      481 gacatagtaa gccgtccagt agccttcctt gtcgaccaga tcttcgtcga tcgccttggc
      541 ttccggcgag cggtaggaag ccaagagctt tctctccatc aggggcacat acatctcggc
      601 gcggccggcg acgacatccc aggcgtgacg cccggcgcgc gcctcgttga gaattttatt
      661 gagcagcggg ccgccgccgg tgcgaaacag ggtcggcttg atgaacggat atttcttctg
      721 aaagcgatcc acgacctcct tgctttgatc cagggtcatg gtggtgtaga aaatcacttc
      781 accttctttt tttgctccct cgatcaagcg cggatcgggg ttcgcttgcg cattggcgaa
      841 ctccacggca aaaatcaccg ctaacattgc ggcgctgacg atgacggcga cgcggctctc
      901 cagtttcatg attttcctcc cggctcaaac aacggtcgag cttattacgt gtcgagcgtg
      961 ctctcggcga agagttcttc gattttcatt tggcgcggtg tcaaaccttg ttcacgggag
     1021 tagctggtaa tggtatcgag catggcgcga ttggcggcga tgccgaaggg aaacgggtcg
     1081 tcgccgatta gctcgcgcgt ggtctcgaga tattcctggc cgaagatcag cgccgtcgga
     1141 atctgcgccg ctagtcgatc gcgggcgtag gctttggcgc ggacaaagcc gtcatataat
     1201 ttgccggcga gccacggatg tttgcggtga atgtcgccgc ggatgatgta ggcgtggttg
     1261 accggtagaa atccatgctg ccggtagaag cgcgcgcctt ccgccatgcg atcgggaaag
     1321 agcggtttga tttggctcca atcgccttta ccggcgatgc ggctagagcg gtccagggta
     1381 ttggcttgca gcacccaggg gctcgatacg tgggcgacat ccagttcgtt tgcgagaatc
     1441 gtcgacgcca gactcttgtt gccggcgatg cgctgaaacg atatgcccgg cggcggtttg
     1501 aaaccggtgg cgccaccgtg gctcatctcc tcgctgcgct ccatgtacca gtgaatccga
     1561 tactgcgaca caccgaaatc atgttcgaga atgccgcgca gccacaacgc tgcggtttgc
     1621 tgatattccg ccacgcccaa acgtttgccg acgaggtctt ggggtttctc gatgccggaa
     1681 tcgacatggt aggacaactc ggtttgaaac aagcggcggc tcggaaaagc cggcagagcg
     1741 atcatgtcgg agccctgcga gcgggcgatc aagaacgacg atagagacat ctccgccact
     1801 tcgaactcgc caaacttcaa ttgacgccaa aaggtttcag ccggatgaga gtaagtggga
     1861 atcaattcga ctccttcaac ttcgatcgtg ccgttcatca gcggctcgac tcgttcgtta
     1921 aaggcggaaa taaatcctaa ggtgagttgt tccattttaa ttattttaga aactaagtcg
     1981 gctgcgtaat cttggttgcc gggcacgcgc catgccgcca ccccagggcg tatgccatac
     2041 gcccctacaa agatgtccga actttgcgcc ccttgcgctc tccgcgatta atgattcgat
     2101 tccgagctgg ccggtcacga acggccccta ctttgtgatc cttgtgccct ttgtggtgaa
     2161 aattcttctc cgtttggtat tgccacgccg gttgttgctt acttgtcgta gccgtcgatg
     2221 gaccaaa
//
LOCUS       DASQKS010000349         5139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5635724, whole genome shotgun sequence.
ACCESSION   DASQKS010000349 DASQKS010000000
VERSION     DASQKS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5635724"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VE170_09070"
     CDS             complement(<1..591)
                     /locus_tag="VE170_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ15628.1"
                     /translation="MIKLYTFPPSTNSRKVRIALLEKGLEFERINVDLSKREQKNPDY
                     LKVHPFGQIPALDDEGFVVYDSTIINEYLEDEYPYPALMPRDSEGRARARMMEDFRDS
                     HFNPHFVHIIQESRKPEAERDAQRVDNAKAEITKAFDRTEAELKGKDYLAGAFSLADV
                     AFMANLELLDRFGIPVDPVKYKNTVAWIARLKARPSF"
     gene            complement(732..2588)
                     /locus_tag="VE170_09075"
     CDS             complement(732..2588)
                     /locus_tag="VE170_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ15629.1"
                     /translation="MRFLRQSLSRRRAVNIAVTAVFLALVSVGARPWLLSGQETTAAD
                     ELLTHGKFLAAPELSGRGVGTPGIATARDYIAAEFAKYGLLPGGDNNSYLQSFDVAVG
                     VQVKEPSSLALGREPALRLHEDWLPLGLSSSAKVEAEAVFAGYGITAKDYGYDDYAGV
                     DVRGKIVVVLRYEPPPKNNQSPFRKSPDFSTHAALRTKANNAREHGAVGMILVDMDSR
                     SEPKAELMSLRSSLWRSGNSMVAAQIKRQVADKWFAALGFSLQDLKEKIDRDERPASL
                     ALPGAKLSLQVSLQEERERAANVVAMLPGKESQMQKDYVVIGAHYDHLGLGHYGTRDQ
                     STAGHIHHGADDNASGTAVLLQLARRLSQIEPKPARAIVFVAFSAEELGLHGSRHFVA
                     KWPQSGSIKSMVNLDMVGRLREDRITVFGIRSSPPFDAIVSAGAKQLGLQTNQPDGVG
                     PSDHLSFYNKQIPVLHFFTGSHTDYHRPSDTWDKLNYPGMAKVGDLVLGSVLAIAALP
                     EAPAFVSLPARISSSSTGERRVGGVYLGIIPEYSRSIDGVLLAGVAPESPAAAAGLRE
                     GDVIIQLADKKISNIEDLTDILGAYKPADQVTIMVQRNNRTSALETTLGSRR"
     gene            2766..2945
                     /locus_tag="VE170_09080"
                     /pseudo
     CDS             2766..2945
                     /locus_tag="VE170_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            complement(3048..3749)
                     /locus_tag="VE170_09085"
     CDS             complement(3048..3749)
                     /locus_tag="VE170_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ15630.1"
                     /translation="MKLKDKVALITGGGRGIGKAIAMHYGREGAKLALCARTGAELGQ
                     TVKELRAMNVDAEGWTCDVSLEEPVKDFIDKAHKKFGRIDVLVNNAGVMTRPAPMIEL
                     DVKKWDYTMAVNLRGPFLITQAVLPIMIRQKSGSIINVSSMIGRGAYANFLAYATSKW
                     GLEGFSQTLAAEVRSSNIRVNSVEPGYVATKLTGFNGSKPESVTDVFVYLAADEAKNV
                     TAKMLSSSGWKSQVK"
     gene            3881..4762
                     /locus_tag="VE170_09090"
     CDS             3881..4762
                     /locus_tag="VE170_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15631.1"
                     /translation="MDVLSITGYNRRPPQLVAEFKGFFAKEKLEVRFHETTYAPDHNQ
                     GMAQGKWDFTLSSADTMIARTTTDGVDYMLFMQAEQGLSAYLIGQPGYDAFDKLRGKL
                     LAGDPGDSNLDLIRKKILRKHGIDDTEYDIEIIGSSPVRLEAFLQRRVVAAMLTPPAS
                     DKALAAGGILLASGDDYVPGWPLTCGWTLRPWLVEHRELVVRFIRAWAAATDWLLQPE
                     HREETVQLSMDKEKLNRAAAEIAYSKIVPRARINPSAIQTVIELRKEMGVYQPPFAPP
                     ARFYDDSYWREATQKMA"
     gene            complement(4818..>5139)
                     /locus_tag="VE170_09095"
     CDS             complement(4818..>5139)
                     /locus_tag="VE170_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYJ15632.1"
                     /translation="HWAISAISLIVVAYLIPGIHVDGLGPALIAPLVIGLVNATVGFI
                     LKIVTFPITILSLGIFLLIINALMLMLAAYLVPGFAIAGFWSAFFGAIVMSIVGMILR
                     SLLT"
BASE COUNT         1115 a         1361 c         1514 g         1149 t
ORIGIN      
        1 gaagcttgga cgggctttta gacgggcgat ccaggcgacg gtgttcttat acttgactgg
       61 atccaccgga atgccgaagc ggtcgagtag ttcgaggtta gccatgaaag ctacgtccgc
      121 caagctgaac gcgccggcga gatagtcctt acctttaagc tcggcttcgg tgcgatcgaa
      181 ggccttggtg atctcggctt tggcattgtc gacccgctgt gcatcgcgct cggcttcggg
      241 cttgcggctt tcctggatga tatgaacgaa atgcggattg aaatgcgagt cgcgaaagtc
      301 ttccatcatg cgcgctcgcg cccgaccttc cgaatccctc ggcatgagcg ctgggtaggg
      361 atattcgtct tcgagatact cgttgatgat ggtcgaatcg taaacgacga aaccttcgtc
      421 atctagggct gggatttgac cgaaaggatg aactttgagg tagtctggat ttttttgttc
      481 ccttttgctg agatcgacgt tgatccgctc gaactcgagg cccttctcta acaacgcaat
      541 gcgcaccttg cgcgagttgg tcgatggcgg aaacgtatag agcttgatca ttgacggcaa
      601 cctccttaac ccttcacaac aagcggtata ttagcacaac gttgtaataa gtggagcgat
      661 tttttcattt tctcggcgaa aattacaact tattttcact ctactcggca tcttgctttt
      721 tatagtcgat gctagcgacg ggagcctagt gtggtctcga gcgcgctggt gcgattattt
      781 ctttgcacca tgatagtgac ttgatcggcg ggcttatagg ctccaagtat gtcggtgaga
      841 tcctcaatat tactgatttt tttatcggct aattgaatga ttacatcacc ttcacgcaag
      901 cctgcggcgg ctgccgggct ttccggtgca acgccagcga gtagcacgcc atcgatactg
      961 cggctgtatt caggaatgat tcctagataa acgccgccaa cccgccgctc gccagtgctg
     1021 gaagaggata ttcgagcggg caggctgacg aaggcgggtg cttccggcaa tgccgcaatg
     1081 gccagtaccg agccgagcac gaggtcgcca actttagcca tgcccgggta gttgagttta
     1141 tcccaggtgt cgctggggcg gtggtaatcg gtatggctgc cggtaaaaaa atgcagcacg
     1201 ggaatctgct tgttataaaa cgacagatga tcgctggggc caacaccgtc aggctggtta
     1261 gtttgtagtc ccagctgctt ggcaccggcg ctgacgatgg cgtcaaatgg cggcgacgaa
     1321 cggatgccga atacggtgat ccggtcttcg cgcaatctcc cgaccatgtc caagttaacc
     1381 atggatttta tcgaaccgct ttgcggccac ttggctacaa agtggcgcga gccatgcagc
     1441 cctaattcct cggccgaaaa cgcgacgaag acaatggcac gcgcgggctt gggctcgatc
     1501 tggcttagcc ggcgcgccag ctggagcagc actgccgtgc cggatgcgtt gtcgtcggca
     1561 ccgtgatgga tatgcccggc ggtgctttga tcgcgcgtgc cataatggcc gagaccaagg
     1621 tgatcgtagt gggcgccgat gacgacgtaa tccttttgca tctgcgactc tttgccggga
     1681 agcatcgcaa cgacgtttgc ggcgcgctca cgctcctctt ggaggcttac ctgtagtgac
     1741 agtttagctc ccggcagggc cagcgaagcc ggccgctcgt cgcggtcgat cttttctttt
     1801 agatcttgga gtgaaaatcc taaagccgcg aaccacttgt cggcgacttg ccgtttgatt
     1861 tgcgccgcca ccatgctgtt gccgctgcgc cacaggctgc tgcgcagcga catcagctcg
     1921 gccttcggtt cggaccggct gtccatgtca acgaggatca tgcccaccgc gccatgttcg
     1981 cgggcattgt tggccttggt gcggagcgcg gcatgggtcg agaaatcagg agattttcta
     2041 aatgggcttt gattattttt tggcggcggc tcgtagcgca gcacgacgac gatcttgccc
     2101 cgcacgtcga caccggcata gtcgtcgtag ccgtaatctt tcgcggtgat gccgtagccg
     2161 gcgaagaccg cttcagcttc gacttttgcc gatgaagaaa gcccaagggg cagccaatcc
     2221 tcgtgcaatc ggagcgcggg ctcccggccg agcgccaagc tgctaggctc ttttacctgc
     2281 acgccaacgg cgacgtcgaa gctttgcaga taactgttgt tgtcgccacc gggaagcaga
     2341 ccatatttgg cgaattccgc cgcgatgtaa tcccgcgccg tggcgatgcc cggtgtgccg
     2401 acgccacggc cactaagttc cggtgccgcc aaaaacttgc catgggtcag tagttcgtcc
     2461 gccgccgtgg tttcctgccc tgacagcagc caagggcgcg cgccaacgct gactagcgct
     2521 aaaaaaactg cggttacggc gatgttgact gcgcggcggc gggataggga ctggcgaagg
     2581 aaacgcataa gcctatgcaa gctaacatgt ttgcagcgcg cttgtcgcta ctctggtatc
     2641 gaattaagac ttttgcggcg gaaaatttga aatgttataa aggtatgatc tattgctaaa
     2701 ggagattgaa gatggcgaag tccgttgttg tctatactct acctggctga ggcccctgtc
     2761 acgccgaggt agagtttcta tctcaaaagg gtgtcgagtt cgtcgagaaa aatctccgcg
     2821 ccgacaaagc gatcttgaag gagttccttg accagggctt tcaaagtgcg ccggtgacga
     2881 ttattgacgg ccagtctgtg gtggggtttg accaagaaaa gattgtggaa ctgctcggat
     2941 tgtaaaataa ttaaaccgat cgaatggcgg cgcagtttgg ctaggattgc cgcgccatgt
     3001 cgatggccgt ttgactagct aatcgtccag cgaaaaagtc gaaacgatta ttttacctgc
     3061 gacttccaac cggaggaact gagcattttg gccgtgacat tcttcgcttc gtctgcagcc
     3121 aaatagacga acacatcggt taccgattcc ggcttgctgc cgttgaaacc ggtgagtttg
     3181 gtcgcaacgt aacccggctc cacggaattg actcgaatat tcgacgaacg cacttcggcg
     3241 gccaaagttt gcgagaaacc ttccagcccc catttcgagg tcgcgtaggc taaaaagttg
     3301 gcgtaggcgc cgcggccgat catcgacgag acgttgatga tcgagccgct tttttgcctg
     3361 atcatgatcg gcaataccgc ttgggtaatg agaaacggcc cgcgcaggtt aactgccatg
     3421 gtatagtccc acttcttgac gtcgagctcg atcatcggcg ccggccgggt catgacgccg
     3481 gcattattga ccagcacgtc gatgcggccg aacttcttgt gcgccttgtc gatgaaatct
     3541 ttgaccggct cctccagcga cacatcgcag gtccagcctt cagcgtcgac attcatggcg
     3601 cgtaattctt tgacggtttg accgagctcg gcgccggtgc gagcgcagag cgcgagcttg
     3661 gcgccctcgc ggccatagtg catggcgatg gctttgccga tgccgcggcc accgccggta
     3721 atcagcgcga ctttgtcctt gagtttcatt agggaacctc ggagcgacgg agcaataaaa
     3781 aatcagaatc taccttgaaa taaaatggaa gccaaccatt ttgcgcttga gcaatcggcc
     3841 gggaggcgat aggaagagag ccgcgtagga ggcgcgaatc atggatgttc tgagtatcac
     3901 cggttacaac cgcaggccgc ctcaattggt ggcggagttc aaaggatttt tcgccaagga
     3961 gaagctcgaa gtgcgctttc atgagaccac ctatgcgccc gatcacaacc aaggcatggc
     4021 gcaagggaag tgggatttca ccctaagcag cgccgacacg atgattgcgc gcacgaccac
     4081 cgacggtgtc gactacatgc tgttcatgca agccgagcag ggactgagcg cctatctgat
     4141 cggccagccc ggctatgatg ctttcgacaa actgcgcggc aagctgctcg ccggtgatcc
     4201 cggcgactcc aatctcgatc tcatccgcaa aaaaattctc cgcaagcatg gcatcgacga
     4261 tactgaatac gacatcgaaa tcatcggcag ttcgccggtg cgcttggagg cgttcctgca
     4321 acggcgggtg gttgccgcta tgttgacgcc gccggcttcc gacaaagcct tggccgccgg
     4381 cggtattctc ctggcgagcg gcgacgatta cgtgccgggc tggccgctta cttgtggttg
     4441 gacgctgcga ccttggttag tggagcatcg tgagcttgtg gtgcgattta tacgcgcctg
     4501 ggcagcggcc acggattggt tactgcaacc ggagcaccgc gaggaaaccg tgcagttgtc
     4561 gatggacaag gaaaagctta atcgcgcggc cgcggaaatt gcttactcaa aaatcgttcc
     4621 cagggctagg atcaatccgt cggccataca aaccgtcatc gaactgcgca aagagatggg
     4681 tgtttaccag ccgccgttcg ctccgccggc gcggttttac gacgacagtt attggcgtga
     4741 ggcgactcaa aagatggcat gagtgaagca agacgaggcg aaaaaaccgg agggcgtctt
     4801 ttggttgggt tatttgttta ggtcaatagc gagcgcagga tcatgccgac gatgctcatg
     4861 acgatggcgc caaaaaacgc cgaccagaaa ccggcgatgg cgaagcctgg gaccaggtag
     4921 gcggcgagca tgagcatcag cgcattgatg atcagcagga atatgccgag tgataggatg
     4981 gttatcggga aagtaacgat tttgaggatg aatccgacgg tggcgttgac caggccgatg
     5041 accagcggcg cgatcaacgc cgggccgaga ccatcgacgt ggattcccgg aatcaggtag
     5101 gcgacgacga tcaaactaat ggcgctgatc gcccaatga
//
LOCUS       DASQKS010000350         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2297596, whole genome shotgun sequence.
ACCESSION   DASQKS010000350 DASQKS010000000
VERSION     DASQKS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297596"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VE170_09100"
     CDS             <1..802
                     /locus_tag="VE170_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15633.1"
                     /translation="KHMDELGVSIQVLYPTLFLIPLSPRPEVELALCRGYNRWLADSW
                     KQAPERLRWAAVVPLMSMDQTLAEMRFAKENGACAIFMRGTEGDRLLSDAYFFPLYEE
                     AQRLDLAIGVHAANGSATLYNYYKYESVGFSKFKLNCVGGFHSMVMDRIPDRFPKLRI
                     GFLEISAEWLPYTFTDLKKRFRLKNKELSADFLKQDRIFVACETTDDIPYIVGKLGED
                     NIVIGSDYAHADSATELMAIHNLAADPRLTPALAKKIVSDNARALYGL"
     gene            1149..2213
                     /locus_tag="VE170_09105"
     CDS             1149..2213
                     /locus_tag="VE170_09105"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15634.1"
                     /translation="MPKPVQASSTAIAMSKQPERQGDKILRKLFYRAPLWLIAALLCA
                     GSALAQTIKIPYVSISGFQAPLYLGERAGLFKKNQLDAQLIYMPGGSLIVQTLLSGDV
                     GVASLAPPSAVGAWAKGAELAIVAGGIERALNVLMVNPKIKKIDDLRGRRIAISRFGS
                     LSDVSLRDALAYHKLKTQDISIAQMGGLGERMVALTSGAVDGAILNVDQVYQAEKLGF
                     HVLIDMGKLPLNYSTQGIVVSREFLRAQRGVVKRFLRVYIEGIKIFKTDRALSIETLG
                     RYVKTNDRDVLAKTYDFYREAWESVPAVRREGIQQALDSIPEGKNPKLNLDSLIDNSV
                     IQELEKEGFLRELYPEGVKR"
     gene            2302..3138
                     /locus_tag="VE170_09110"
     CDS             2302..3138
                     /locus_tag="VE170_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15635.1"
                     /translation="MLKTIGLIEGHYECRSLDETLPVFTDLLAMEIVERKAGQATIKH
                     PNTDWRLIVHEGGPDAPEKTFDNHYGFRVASHTEVEAAWEYVEAHKDKYRLSKITKPQ
                     SAHFAYSIYFHEPGGNHLEIEYYNPGGALHGRAHTAGHWDNTLNSERFPGRGYVPQAF
                     THGTLQCDDLERSRRFYVEVLGLEIAAGFNTAQYIKHPATPWYLVVLQRSPRQYLAPV
                     NRFTLRVGSAAEVEQAHREFVSHKNALGLNEISDIDRADGTVNFIFSDPDKNWWELTA
                     NG"
     gene            3143..4192
                     /locus_tag="VE170_09115"
     CDS             3143..4192
                     /locus_tag="VE170_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15636.1"
                     /translation="MIKLAQLTGCLLAVCVAAGAQAQAPFYEGKNILLVSGTDAGGAG
                     DLRTRAILAPLKKHIPGHPNIVVQYMPGAGGRRAANYLYKSVKPDGLTMGAMITGMIQ
                     GTLFGETDVLYDIDKFIYLGSHARYAPYGFISRKGAGFTTKEKLAAATGIRIGAPSVG
                     HSLYTMARLFAYMFDLKEPRFVTGYTPREIDFALERNEIDARVSILDSLLTRNAHWLE
                     KDMVDVNATMDIPKDLSYSHPRLAKLLEIEALARSNQERRLIALFRGARGTGQPFVFP
                     PATPPDRVKILREAMRNAFADADYVKSYEKLTAEPAMPITPEEFEKQLREIPREPEII
                     DLFKKLSGPGPLPKR"
BASE COUNT          981 a         1340 c         1151 g          842 t
ORIGIN      
        1 caagcatatg gacgaactcg gcgtcagcat ccaggtgctc tacccgacgt tatttctcat
       61 accgctatcg ccgcgccccg aagtcgagct ggcgctttgc cgcggctaca accgctggct
      121 cgccgatagc tggaaacaag cgccggaacg gctgcgctgg gccgccgtcg tgccgctgat
      181 gtcgatggat caaaccctgg ccgagatgcg cttcgccaag gaaaacggcg cctgcgctat
      241 cttcatgcgc ggcaccgaag gcgaccgtct gttgagtgat gcttatttct ttccgctcta
      301 cgaagaagcc cagcgcttag atctcgccat cggcgtccat gccgccaacg gcagcgcgac
      361 gttgtataac tattataaat acgaaagcgt cggcttctcg aagttcaagc tcaactgcgt
      421 cggcggcttt cactccatgg tgatggaccg catccccgac cgctttccca agctgcgcat
      481 cggtttcttg gaaatcagcg ccgagtggct gccctacaca ttcaccgatc taaagaaacg
      541 cttccgcctg aagaataaag aactctccgc cgactttttg aagcaggacc gcatcttcgt
      601 cgcctgcgag accaccgacg atattcccta catcgtcggc aagttgggcg aagacaatat
      661 cgtcatcggc tccgactatg cccatgccga cagcgccacc gagttgatgg caatccacaa
      721 tctcgccgcc gacccgcgtc tgacaccggc gctggcgaaa aagatcgtca gcgacaacgc
      781 cagggccctg tacgggctgt aatacaagaa acaaattcgg aagattggcc gcaaagagcg
      841 cataggacac aaaaatttaa ttctgaatac taaaggtgag tttgaaaccc gccggtttgg
      901 tttgtagtgc gaccggccat cgcgagagga agggcgatca tgatcaggct cggcgagttg
      961 tgtcaggttt ttagttttct tatcgccagc ggattttttc tcgcgccgcg ctcagccgga
     1021 gccgccgaga ccgcgaacct cacgccgctg cgccgaccct cgccgttttg tcgacttcac
     1081 gatattggat gaattggaaa gaaggctttg tcaaagctct ctccaagaac taaattgctg
     1141 cggtgatcat gccgaagccc gtccaagcaa gctcgacggc gattgccatg agcaaacagc
     1201 cggagcggca aggagataaa attttgcgaa aactttttta tcgcgcgcca ttgtggctca
     1261 tcgccgcgct gctgtgcgcc ggctctgccc tagcccagac cattaaaatc ccctacgtct
     1321 cgatctccgg ctttcaggcg ccgctctatc tcggcgaacg cgccggcctg ttcaagaaaa
     1381 accaactcga cgcccagctg atttatatgc cgggcggttc gttgatcgtg cagaccttgt
     1441 tgtccggcga tgtcggcgtc gccagtcttg cgcctccctc agcggtcggc gcttgggcca
     1501 aaggcgccga gctcgccatc gttgccggtg gcatcgagcg ggcattgaac gtcctcatgg
     1561 tcaatccaaa aataaaaaaa attgacgacc tcagaggcag gcgcatcgcc atcagccgtt
     1621 tcggctcgct ctccgacgtc tccctgcgcg acgcgctggc ctaccacaag ctcaaaaccc
     1681 aagatatctc aatcgcccag atgggcggtc tgggcgaacg catggtggcg ctcacctccg
     1741 gcgccgtcga cggcgctatc ttgaacgtcg atcaagtcta ccaggccgaa aaactcggct
     1801 ttcacgtgct catcgacatg ggcaagctgc cgctcaatta ttcaacccaa ggcatcgtcg
     1861 tcagccgcga gtttctgcgc gcgcagcgcg gcgttgtcaa acgattttta agagtttata
     1921 tcgaaggcat caagatcttt aagaccgata gggcattgtc catcgaaacc ttgggccgct
     1981 atgtcaagac caacgaccgc gacgtgttgg ccaaaaccta tgacttttac cgcgaggcgt
     2041 gggagagcgt gccggcggtc aggcgtgaag gcattcagca ggcgttggac agcatccccg
     2101 aaggcaagaa tcctaaattg aatctcgata gcctgatcga caactcggtc attcaagaac
     2161 tggaaaagga aggctttcta agagaactct atccggaagg cgtgaagcgc tgacgtcacc
     2221 accgagcgcc aactgcgcca gttgacagtg caccgctccc aacgttaatg aatctgcagg
     2281 tcatccgcag aggagaaagg catgctcaaa accatcggcc tgatcgaggg ccactatgaa
     2341 tgtcgttcgc tggacgaaac cctgccggta ttcaccgacc tgctggcgat ggagatcgtc
     2401 gagcgcaagg cgggtcaagc gacgatcaaa catcccaaca ccgactggcg cctgatcgtc
     2461 cacgaaggcg gcccggacgc gccggagaaa accttcgaca accactacgg ttttcgcgtc
     2521 gcctcgcaca cggaagtcga agccgcctgg gaatacgtcg aagcgcacaa agacaagtat
     2581 cgcctcagca agattaccaa accgcagagc gcccacttcg cctactcgat ttattttcac
     2641 gaacccggcg gcaatcattt ggaaatcgaa tattacaatc ccggcggcgc gctgcacgga
     2701 cgcgcgcata cggcgggcca ttgggacaat actctaaaca gcgaacgatt ccccggtcgc
     2761 ggttacgtgc cccaggcttt tacccacggc acgcttcagt gcgacgacct cgaacgcagc
     2821 cggcgttttt acgtcgaagt gctcgggctg gaaatcgccg ccggtttcaa caccgcccaa
     2881 tacatcaaac atccggcgac gccgtggtat ctcgtcgtcc tgcaacgcag cccgcgtcaa
     2941 tatctggcgc cggtcaatcg cttcaccctg cgagttggct cggcagcgga agtcgaacaa
     3001 gcgcaccggg agtttgtgtc gcacaagaac gcactgggcc taaatgaaat ctccgacata
     3061 gatcgcgccg acggcacagt caatttcatc ttcagtgatc ccgacaaaaa ttggtgggaa
     3121 ctcaccgcca acggttaaac ccatgattaa acttgcacag ctgaccggtt gcctattggc
     3181 ggtctgcgtc gccgccggcg cacaagctca agcgccgttt tacgaaggga aaaatattct
     3241 cctggtcagc ggcaccgacg ccggcggcgc cggcgatctg cgcacgcggg cgatcctcgc
     3301 acctctgaag aaacacattc ccggccatcc caacatcgtc gtccaataca tgcccggcgc
     3361 gggcggccgc agagcggcca attatcttta caagtcggtg aaacccgacg gcttgaccat
     3421 gggcgccatg attaccggta tgattcaggg caccctgttc ggcgagaccg acgtgctcta
     3481 cgacatcgac aagttcatct atctgggctc ccacgcgcgc tacgcgccct acggctttat
     3541 cagccgcaaa ggcgccggct tcacgaccaa ggaaaaactc gccgccgcca ctggcatacg
     3601 catcggcgct ccctccgtcg gtcacagcct gtacaccatg gcgcggctgt tcgcttacat
     3661 gttcgaccta aaagagccgc gcttcgtcac cggctacacg ccgcgggaga tcgacttcgc
     3721 cttggagcgc aacgagatcg acgcccgagt gagcatcctg gacagcttac tgacgcgcaa
     3781 tgcccattgg ctcgaaaaag acatggtcga cgttaacgcc accatggaca tacccaaaga
     3841 ccttagttac agccatccgc ggctggctaa acttctcgag atcgaagccc tggccaggtc
     3901 taatcaggaa cgccgcttga tcgccctatt tcgcggggcg cgcggcaccg gccagccgtt
     3961 tgttttccca cccgccacac cgcccgaccg tgtaaagatt ttacgggaag ccatgcgcaa
     4021 tgccttcgcc gatgccgatt acgtcaagag ctacgagaaa ctcaccgccg agccggccat
     4081 gccgattacc cccgaggagt tcgagaaaca gctgcgcgaa atcccgcgcg agccggagat
     4141 catcgatcta ttcaaaaagt tatccggccc cgggccgctg ccgaagcgct agccggtaac
     4201 atcacatcgt tacccatgag tctcggctgg ccgccgcgtg tgtgggagat acaacgcaat
     4261 gttgtcatcc gttgaagtcg ttgcaagttc gcgtcagccc tctttggaaa agag
//
LOCUS       DASQKS010000351         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4802117, whole genome shotgun sequence.
ACCESSION   DASQKS010000351 DASQKS010000000
VERSION     DASQKS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4802117"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VE170_09120"
     CDS             <1..525
                     /locus_tag="VE170_09120"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ15637.1"
                     /translation="DAEQMGFDDAAFDCVTCGFAVWMFAEPARVLQEFRRVLRPGGKV
                     ALSSWAADNPAQTWCHEVLRPFVAAFASKLSAPKDVRFDKPLQLETVLRQSGFADIQI
                     TVEEKEFVYRSEEQYWSSLWSSGLRRPLEKMPADLLARAKSDVLRKFQTFRQPDGFHK
                     LSRALFACGRKPTM"
     gene            643..1593
                     /locus_tag="VE170_09125"
     CDS             643..1593
                     /locus_tag="VE170_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15638.1"
                     /translation="MKNTFLVMTTLYLLHASVQAAEKIRIGFPEFNSSTFTLPMAQIK
                     GFFHEEGLQAELIRIRSAVALPALTSGEIDYHTVVAPAVTAALRGIPVRVVACFTPGL
                     TTAIIARSEIKTVQDLKGKTIGLNSIGGGLESTVRLMLKHYGLDPDKDVKFLATGGIE
                     TRFAALKQGLTVATAGSTPADYLGKKFGYIVLARAYDLFSYPNTGLVASLKKIKESPD
                     QLKRVIKAGIKANRYILQNRDGTIQVMMQWMRVDRDTAIATYDGSVKSFNDDGAVSDA
                     GLRIALDESRKAANMTREVAFSEIADFSMLREAQRELATK"
     gene            1692..2645
                     /locus_tag="VE170_09130"
     CDS             1692..2645
                     /locus_tag="VE170_09130"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15639.1"
                     /translation="MAILVLLLLMFFTLSAPVRAADKIRISMTGFAGQFMTFPLAQKR
                     GFLKEEGLETEIVRISAAAGRAALTNGDIDYSTGIGGTAINGALSGIPIRVVACFVPA
                     PVLGLVARPEYKSVQALRGKTIAILIPGGVAHFAARAMVRHAGLDPEKEMKYLAVGPP
                     DARYAALTQGLVEAAVLGPPLDFEARKQGYNILLRADEVMVLPETGLVAGVKKIHDKP
                     DEIKRVIRAGIKANRYIRANRDGTVQFLTEWLKLNRESATATYDSVVRVYNEDVNVCD
                     KGLRVVIDETKKTMKLSRDVAYSEVADLAIFRDTQRELAGK"
     gene            2712..3536
                     /locus_tag="VE170_09135"
     CDS             2712..3536
                     /locus_tag="VE170_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HYJ15640.1"
                     /translation="MISKTLIRILVAGVFLLLLVVAKPFVIVRAGTVGVVYSFGSIVG
                     QVDEGFHLIWPWWFITHTDIKVQRAVLDKLPSFSAETQDVFVRASLNYQVSPKAVQTL
                     FRTVGPDYFHVLIEPRVAQNFKDETVKYKSVDIAPNREKIRQAVRQRLEAELSPYSIH
                     IIDLLLDNVDFSAAFKQSIENKQIATQKALEQEQLVSAAKFSAQQRVETEKGEGEAKL
                     ARAEKEALANQRLSASLTPLLVQHEMIDKLSEKIQIMVVPQGSNFLMQLPNIAAVK"
     gene            3554..>3721
                     /locus_tag="VE170_09140"
     CDS             3554..>3721
                     /locus_tag="VE170_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15641.1"
                     /translation="MDLGLRGKVAIITGSSDGIGFATARGLAHEGTRVVLCARREALL
                     TQARDAIAIETG"
BASE COUNT          866 a          980 c         1091 g          784 t
ORIGIN      
        1 gatgccgagc agatggggtt tgacgacgct gcatttgatt gcgtcacctg cggttttgcg
       61 gtctggatgt ttgccgaacc ggcgcgggtt ctgcaggaat ttcgtcgcgt actgcggcca
      121 ggcggaaagg tagctctcag cagttgggcg gcggataatc cggcccaaac ttggtgtcat
      181 gaagtgcttc gaccctttgt agcggcattc gcgtccaaac tgtcggcgcc gaaagacgtg
      241 agattcgaca aaccgttgca gttagaaacg gtgctgcgac agtcaggctt cgcagatatt
      301 caaatcacag tcgaagagaa ggagtttgtt tatcgcagcg aggaacagta ttggtcttct
      361 ctctggtcgt ccggtttacg gcgcccgctt gaaaagatgc ctgcggattt actggcacgg
      421 gccaagagcg atgtcttacg aaaatttcaa acatttagac agccagatgg atttcacaaa
      481 ctgagccgcg ctttattcgc ttgcggaagg aagccgacga tgtgacacga tgcgttgtct
      541 gtaattgagg ggggctagat ttgcagagat gccaagaacc aagaaccaac ggtgcccttg
      601 agaccttggc ctcactatac ggtcaagcga aagggtaatt ccatgaaaaa cactttcttg
      661 gtgatgacga ctctgtatct gcttcatgca tccgttcagg ccgccgaaaa gattcgcatc
      721 ggctttccgg aattcaactc ttcgaccttt acactgccga tggcgcagat caaaggcttt
      781 tttcatgagg aagggctaca agcggagctg atccgaatac gttcagccgt ggccctgccg
      841 gcgctcacca gcggggaaat cgattaccac accgtggtcg ctcccgccgt tacggcggcc
      901 ctgcgcggca taccggtgcg ggtcgtcgct tgtttcactc caggtctgac cacggcgatc
      961 atcgcgcgtt ccgagatcaa gacggtccaa gatctcaaag gcaagaccat cggcctcaat
     1021 agcatcggcg gcgggctcga aagcacggtg cggctgatgt tgaagcacta cggtctagat
     1081 cccgacaagg atgtgaaatt cctcgccacg ggtggtatcg agacgcgctt tgcggccttg
     1141 aaacaaggcc tgaccgtggc gaccgcgggc tcaacgccgg cggactattt gggcaagaaa
     1201 ttcggctaca tcgtgctcgc ccgagcctac gacctgttca gctatccaaa caccggattg
     1261 gttgcgagcc tcaaaaaaat caaggaaagc cccgatcagc tcaagcgtgt gatcaaagcc
     1321 ggtatcaaag cgaatcgtta catattgcaa aatcgcgacg gcacgattca ggtcatgatg
     1381 cagtggatgc gagtcgaccg ggacaccgcc atcgcgacct acgacggctc ggtgaagtcg
     1441 ttcaacgacg atggcgccgt ctccgacgcc ggactgcgca tcgcgctcga tgaatcacgc
     1501 aaggccgcta acatgacccg ggaagtggcc ttcagcgaaa tcgccgattt ttccatgttg
     1561 cgtgaggcgc agcgggaact ggcgactaag taaatttaaa tccttaaggt tagattttgg
     1621 attgtcgtca aactgcgacg gggaacaaga atatgcgcga caaagcccta aacgcgattc
     1681 gacagcacat tatggcaata cttgttctcc tgctgctgat gtttttcact ctcagtgcgc
     1741 cggttcgggc agcggataag atcagaatct ctatgaccgg tttcgccggt cagttcatga
     1801 cctttccgct ggcgcagaag cgcggctttc tcaaggagga aggcctcgag acggagatcg
     1861 ttcgcatctc ggcggcggcc ggccgcgcgg cgttgaccaa cggtgatatt gactattcca
     1921 cggggatcgg cgggacggcg atcaatggcg cgctcagcgg cattccgatc agagtcgttg
     1981 cctgctttgt tcccgcgccg gtgctcggtt tggtggcgcg gccggaatac aagagcgtgc
     2041 aagcgctcag aggcaagacg attgctattt tgattcccgg cggcgttgcc cattttgccg
     2101 cgcgagcgat ggtcaggcat gccggtctcg atccggaaaa ggaaatgaaa tatctcgcgg
     2161 tgggcccgcc cgatgcccgt tacgctgcgc tgactcaggg cctggtcgag gccgcggtgc
     2221 ttgggccgcc gttggatttc gaggcacgca agcagggtta caatattctg ttgcgcgccg
     2281 acgaagtgat ggtgctccca gagaccggcc tggtcgccgg ggtgaagaaa attcacgaca
     2341 agccggatga gatcaaacgg gtcattcgcg ccggcatcaa ggccaatcgt tatattcgtg
     2401 ccaatcgcga tggcacggtg caattcttga cggagtggct caagcttaat agagaatctg
     2461 ccacggctac ctacgatagc gtcgtcaggg tctacaacga agatgtcaac gtctgcgaca
     2521 agggtctgcg cgtggtcatc gatgaaacca agaaaaccat gaaactcagc cgcgatgtcg
     2581 cttacagcga agtcgccgac ctggcgattt ttcgggacac gcagcgggaa ttagccggca
     2641 agtagtggaa caaactcaag cgactcggcc ggggcaacta tcatgcatag gcgaatttga
     2701 aaggactaac gatgatttcg aaaactttga taagaatatt ggtcgccggg gttttcctgt
     2761 tgttattggt agtcgccaaa cccttcgtga tcgtgcgcgc gggcacggtc ggcgtggttt
     2821 acagttttgg ctccatcgtc ggccaagtgg acgagggctt tcacctcatt tggccgtggt
     2881 ggtttatcac ccacacggat atcaaagtgc agagagcggt gttggataaa ctgccgtcct
     2941 ttagcgcgga gacccaggat gtcttcgtca gggccagctt aaactatcaa gtctcaccca
     3001 aggcggtgca gacgttgttt cgcacggtcg gaccggacta cttccatgta ctgatcgagc
     3061 cgagggtggc gcaaaacttc aaggacgaaa ccgtgaagta taagagcgtc gacattgcac
     3121 ccaaccgaga aaagattcgg caggcggtgc gccaacgact cgaagcggaa ctttcaccgt
     3181 actcgatcca tattatcgac ttactgctcg ataacgtgga ctttagcgcc gccttcaaac
     3241 aatccattga gaacaaacaa atcgccacgc agaaggcact cgagcaagag caacttgtct
     3301 cagcggctaa gttttccgcc caacaacggg tagagaccga aaaaggcgaa ggcgaggcaa
     3361 agctggcacg ggctgagaaa gaagcgctgg ccaaccagag actgtcggca tcgctgacgc
     3421 cgttgctcgt ccagcacgaa atgatcgata agttgtccga gaaaattcaa atcatggtcg
     3481 tgccccaagg atcgaacttt ctcatgcagt tgcccaacat cgcggcagtg aaatgagtgt
     3541 ataaggaggg ccgatggatt taggtctgcg cggcaaagtt gccattatca ccggctccag
     3601 cgacggaatt ggctttgcga cggctcgtgg cctagcgcac gagggcacgc gagtcgtgct
     3661 gtgcgccagg cgcgaggcgc tgctaaccca ggcgcgcgat gccatcgcca tagagactgg
     3721 a
//
LOCUS       DASQKS010000352         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5636305, whole genome shotgun sequence.
ACCESSION   DASQKS010000352 DASQKS010000000
VERSION     DASQKS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5636305"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VE170_09145"
     CDS             complement(<1..613)
                     /locus_tag="VE170_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYJ15642.1"
                     /translation="MKPTRDTRQLNWFLAWAVVFCDIGTSVYYVPGILYGHVKDATPF
                     FVLLTTGGFILLSLKYIEISWRNPEGGGVVTITTKAFGPIWGCLGGMLITVDYFLTTA
                     ISAVAGFQYVGSAFPALDAHIVLLACMGVGVLAALNIVGIRESATVALVMACASFFVN
                     IAVMATALITMDGAQWSTVLSRLGTGKEISHYELLVGFSSAWLA"
     gene            complement(703..2541)
                     /locus_tag="VE170_09150"
     CDS             complement(703..2541)
                     /locus_tag="VE170_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYJ15643.1"
                     /translation="MEPTRDTRQLTWLLAWAVVFCDIGTSIYYVPGILYGNVGDNTPF
                     FVLLTTLGFIPLALKYIEISWRNPEGGGVVTIATKAFGPMWGCLGGMLITTSYFLTAA
                     ISAVSGFHYIASLVPFIDSHIIWLACLGLVALAMLNVVGIRESATVALIMALTAFLVN
                     LVVGAVAVWQFSPAQLWRALARFTPDQSMSYRELLVGFAAAWLAFSGLESISQLTPNM
                     RFPLRRTARWAMLAVIVTTLLTSPLLTLLSIHLLSAEFKATQSERFISEVAMVSGGLG
                     LKLAVVMSASTLLLFAANTAIIGAYHIFLALTRLEFLPRIIAIRSAHFNSPHLAIFIA
                     TGIPIAVILATQGKLALLGEMYAFGLLGAFVFSSLSLDVIRWRLGRRDLAFWIGVLTT
                     VMIAVAWCINLVEKELATVFGGALTLIGMTVAVGVRRAWFLDLLHKIPWIQRLQARAY
                     QASEALVDEELKGLVRLADAVEIKALYSSSTLMALRGENPRLMQEGIRRSKGAGETAL
                     YCVYVEEWPGLFDGHTPHRPNEEGLRTLRAAVQETRGKNIEAIPIWMVSHNAAEAIAN
                     AAQALDVDTVMIGASRRSAFYHMLRGHVIKGLMKRLPHDCHVMICN"
     gene            2648..3766
                     /locus_tag="VE170_09155"
     CDS             2648..3766
                     /locus_tag="VE170_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ15644.1"
                     /translation="MALPVQYFQKLIESSPDIIISVDKSGTITFYNDGARQNLGFTSD
                     EVLGKNVAEIYPSIEEARQVMAAMRANDGEGKGRVRNFETVCKTKRGEQIPVAISASI
                     LYDDQGQEIGSIGFAKDIREIRRRDRLVTLAEIAIGLSHEINNCLEVLVNQSEMMKKY
                     VERVATDEDYIVESDRLESVASQVRKIQDITARMAKMAEEGEYGTTEYLDGKMMTDLH
                     VDAAAGEKPCARPPPDACFPLSGLNVLVVDDDAGICQSLKQLLESERCCIETAASGVY
                     AIEWLGRRRFDVVLSDVVMPDMDGYELYQFVKKNTPELPVVLMTAFNYDKDHIIKRSC
                     LEGLQGVIFKKPVNPTMLKKVLLQQCRPEPVPPPAASS"
     gene            3800..4135
                     /locus_tag="VE170_09160"
     CDS             3800..4135
                     /locus_tag="VE170_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ15645.1"
                     /translation="MNKEDHYNQGMAHFAEDRLDDAVAAYLHALDEDPNYADALHALA
                     MTYAHQEKVDQAIDVAKRLIAAAPEDELAYTSLSIFYQQKGMIAEAEEVGAKARTLGW
                     KRQLSENKR"
BASE COUNT         1042 a         1164 c         1235 g          821 t
ORIGIN      
        1 acgccagcca cgccgaagaa aatccgacca gcagctcata gtgagagatt tctttgccgg
       61 tgcctagacg gctgaggacc gtgctccact gtgcgccgtc catggtgatc aacgccgtgg
      121 ccatgaccgc tatgttgacg aaaaatgaag cgcaggccat gactagcgcg acggtggcgc
      181 tctcgcgaat gccgacaatg ttaagcgcgg ccaagactcc gacgcccata caggcgagca
      241 agacgatgtg ggcgtccaac gccgggaagg cgctgccgac atattgaaag cccgcaaccg
      301 ccgagatcgc ggtggtcaaa aaatagtcca ccgtgattag catgccgccc aggcagcccc
      361 aaatcgggcc gaaggctttg gtcgtgatcg tcacgacgcc gccgccctcg gggttgcgcc
      421 aggagatttc gatgtattta agcgatagca ggatgaagcc gccggtggtc aagagcacaa
      481 aaaacggcgt agcgtctttg acatgaccgt aaagaatgcc cgggacgtaa taaaccgagg
      541 tgccgatatc gcagaacacc actgcccagg ccaaaaacca attgagttgc cgagtatcgc
      601 gggtgggttt catagagtgt cgacgaagat cgcttaggaa tagatgccca ccgtcggtaa
      661 ggcaagcgtg attgcgaacg tcacgaaagc gcgcgaacga tgctagttgc agatcatcac
      721 atgacaatcg tggggcaaac gtttcatgag acctttaatc acgtgaccgc gcagcatgtg
      781 gtaaaacgcc gatcgcctgc tcgcgccaat catgactgtg tcgacgtcaa gggcttgggc
      841 ggcgttcgca atcgcctcgg cggcgttgtg cgataccatc cagatgggta tcgcttcgat
      901 atttttgccg cgtgtttcct gcacggcggc gcgcaatgtg cgcagccctt cctcgttggg
      961 ccgatggggc gtgtgcccgt caaacagtcc gggccattcc tcgacatata cgcaataaag
     1021 ggccgtctcg cctgcgccct tggatcggcg aatgccttct tgcataagcc gtggattttc
     1081 gccgcgcaag gccattaggg tagaggagga ataaagggct ttgatttcga cggcgtcggc
     1141 cagtctgacc agccctttta gttcctcatc gaccaaagct tccgaggctt ggtaagcacg
     1201 ggcttgcaag cgctggatcc aaggaatctt gtgcagcaga tcgagaaacc atgcgcgacg
     1261 cacgccgacg gcgacggtca taccgatcag ggtcaaggcg ccgccgaaga cggtggccaa
     1321 ttccttttcg accagattga tgcaccaggc cactgcgatc atgacggtgg tcaaaacccc
     1381 gatccaaaaa gccaggtcgc ggcgcccgag acgccaacga atgacgtcca gactgagcga
     1441 cgaaaagaca aaggcgccga gcaacccgaa cgcgtacatc tcgccgagca gcgcgagctt
     1501 gccttgggtg gccaaaatca ccgcaatggg gattcccgtt gcgatgaaaa tggcgaggtg
     1561 tgggctattg aagtgggcgc tacgtatggc gatgattcgg ggcaaaaatt ctaaacgggt
     1621 caatgccaga aaaatatgat aggcaccgat gatcgccgtg ttggcggcaa agagcagcaa
     1681 cgtcgaagcg ctcatgacca ccgcgagttt aaggccgagc ccgcccgaga ccatggcgac
     1741 ttcggaaata aaacgttcgc tctgggtcgc cttgaactcg gccgacaaca gatggatcga
     1801 taacagggtc aacaatggcg aggtcaagag cgtggtcacg atcacagcta acatcgccca
     1861 gcgcgctgta cggcgcagcg ggaagcgcat gttcggagtc aattgactga tactttccaa
     1921 gccggagaac gccagccagg ctgctgcgaa gccaaccagc aattctcgat aggacattga
     1981 ttgatccggc gtgaaacggg ccagcgctcg ccacaactgc gccggagaaa actgccagac
     2041 ggcgacggcg ccgacaacga gattcactaa gaatgccgtc agcgccatga tcagggcgac
     2101 agtagcgctc tcgcgaatcc cgacgacgtt taacattgcc aatgcgacga gtccgaggca
     2161 ggcgagccaa atgatatggc tgtcgatgaa aggcaccagg ctggcgatgt agtggaagcc
     2221 cgaaacggcg gagatcgccg cagtcagaaa ataactggtg gtgatcagca tgccgcccag
     2281 gcatccccac ataggaccga aggctttggt ggcgatggtg acaacgccgc caccttcggg
     2341 gttacgccaa ctgatctcga tatacttcag ggcgagcggg atgaagccca aagtcgttag
     2401 caagacgaaa aaaggagtgt tatcgccgac gttgccgtag aggatacccg gcacgtagta
     2461 gatcgaagtg ccgatgtcgc aaaaaactac cgcccaagca aggagccagg tgagttgtcg
     2521 agtgtccctc gtgggttcca tgttgccggt aaaatggttc gggggtgctc cgcttcgagg
     2581 gccagctgat aggctcttgc attgataacg ccgttcgact tatcatgcaa ctcaacatcc
     2641 cgatcctatg gccctgccgg ttcaatactt tcagaagctc attgagagtt ctcctgacat
     2701 catcatctcg gtcgacaagt ccggcacgat tactttctac aacgacggag cgcggcagaa
     2761 tctcggattt acttccgacg aagtgctcgg caagaatgtc gcggagatct acccgagtat
     2821 cgaggaagcg cgccaagtga tggcggcgat gcgtgccaat gacggagaag gcaagggccg
     2881 ggtcagaaac ttcgaaaccg tctgcaagac caaacgcggc gagcagatcc cggtcgcgat
     2941 ctccgcgtcg atcctctacg acgatcaggg ccaggaaatc ggttctatcg gttttgccaa
     3001 ggatattcgg gagatccgcc ggcgcgaccg tttggtcact ctcgccgaga tcgcgattgg
     3061 gctatcccac gagatcaata attgtttaga agtcctggtc aatcagagcg agatgatgaa
     3121 gaaatatgtc gagcgagtcg ccaccgatga ggattacatc gtcgagtcgg accgcctcga
     3181 atcggtggcg tcccaggtgc gcaagattca agacattacg gcacgcatgg ctaaaatggc
     3241 tgaagagggc gagtacggca cgacggagta tctcgacggc aagatgatga ccgatctcca
     3301 tgtcgatgcc gcggctggcg aaaagccttg cgctcggccg ccacccgatg cgtgttttcc
     3361 gttgagtggt cttaacgttt tggtggtcga cgacgacgca ggcatctgtc aatcgctcaa
     3421 gcagttgctc gagtcggagc gctgctgcat cgaaacggcg gcatccgggg tctacgccat
     3481 cgagtggctc gggcggcgcc gtttcgacgt cgtcctgagc gatgtcgtca tgcctgatat
     3541 ggatggttac gagctctatc aattcgtcaa gaagaacacg cccgagcttc cggtagtctt
     3601 gatgactgcg ttcaattacg acaaagacca cattatcaag cgcagttgcc tggaaggact
     3661 gcaaggtgtg atcttcaaaa aaccggttaa tccaactatg ctaaaaaaag tattgctgca
     3721 gcaatgccgg cccgaaccgg taccgccgcc cgccgcgtct tcataaactt aaaagtcaat
     3781 cggtcaactg accgttctca tgaataaaga agatcactac aatcagggaa tggcgcattt
     3841 tgctgaagat cggctggacg atgccgttgc cgcatatctt cacgccctcg atgaagatcc
     3901 taattacgcc gacgcgttgc atgccctggc gatgacctat gcgcaccaag agaaggtcga
     3961 tcaagccatc gacgtagcga aacgcttgat cgcggcggcg ccggaagatg aactcgccta
     4021 taccagcttg tcgatcttct atcagcagaa gggcatgatc gccgaagcgg aagaggttgg
     4081 cgctaaagcc cgtaccctcg gttggaagcg ccagctctcc gaaaacaagc gttagtccga
     4141 gttttgccaa caattaccgg taggacgaag aatttcaccg tcgacacact acagggcagt
     4201 gtctgcccgc gcaaatcgcc gcatccgatc cctcccattg gaccattgta gttagttttt
     4261 tt
//
LOCUS       DASQKS010000353         7919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_608467, whole genome shotgun sequence.
ACCESSION   DASQKS010000353 DASQKS010000000
VERSION     DASQKS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608467"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VE170_09165"
     CDS             <1..765
                     /locus_tag="VE170_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15646.1"
                     /translation="RAADYDIVYLQFCGRSKIESEGGVIEVEPGEIALVPSAIAHRST
                     GTEQCLRVRVATQEAMTEGVDPEKPLTQNKFSVKPSEPIGAANGHNADGAGDGTALEH
                     ISFWDPQSDLWVEREVAALIGCKREGGRPVRKLSAFNYFTGMTGKGGARAPILYNGKE
                     FRVDVYNLEEEQNGFHRGCDEDEIWFQFRGHSLNHTEWGAMELDAGQMSYIPRGVAHR
                     ITGSPGFLRMVLYSRKLVHPKAFNNISERATRFDVQ"
     gene            882..1994
                     /locus_tag="VE170_09170"
     CDS             882..1994
                     /locus_tag="VE170_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15647.1"
                     /translation="MIGGKPKISDLVIGARNMIEAADIRPGDSVLMLADRSSDGDAVD
                     ALAAALRLHGATPMEMIVEPILRYGDVPEAALRAMEAVDVAIWVWPVFLNGSTNYRER
                     LRTPKEGDAGAPTERVKPYKIYFEGWPGLLATDYARFPNELLWTIARKVKAIVAAGSK
                     VTISGPNGAKLVAEYDNKRIYAMQTKAGEPAGRCHFPWGRCGVYNGSGTANGTVQLDC
                     AQSVPGMLREPMVWTIKDAKVVGVEGGGDIGAECKRLFKAFPGSDFFTEVMFGYHPKA
                     STERGIQDPMHWEILSRYPWVGLGTSRKEMVYRHVDGGCMESNLAIDGRVLVDFGGKL
                     TILDDAEIRAAAARYGDPDFLLSTLSHEGTALGGLW"
     gene            2129..3244
                     /locus_tag="VE170_09175"
     CDS             2129..3244
                     /locus_tag="VE170_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15648.1"
                     /translation="MKRLGLVVLVSLISLANHPGFILAQQPWQAEWDKTVAAAKKEGK
                     VVVGLPPSAELRRELEPAFKARFGFEMEIFSATGPQIANRIVTEGKAGVRYFDSFIFG
                     SCTGVPLIKSGIFDPIEPYMILPEVKEPKQWWSGHVFMDNVSGTKLFYSFISTKSTEG
                     YWYNTSLAKAEEFRSLDDLLNPKWKGKIGLSDPRVAGSGLSVWSYLWDLKGEEFLKKL
                     VQQDLFVTQNLRQLAEALAKGKLAITLGIGLAQTEPFVKAGLPIKDLPDTSEGNPSSN
                     GYGTLAVVKNSPHPNATKVFVNWLLSKEGQEIYSRVLLHGTRRLDVDTKWMAKQGVEA
                     SKDTLSPKDNEKMRNYLEDKCVEVRLKAQKFAEKVLQ"
     gene            3336..4790
                     /locus_tag="VE170_09180"
     CDS             3336..4790
                     /locus_tag="VE170_09180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15649.1"
                     /translation="MDEIPYKDFRGFIEAAKNVSAWRLIEGADWNNEIGALIEATAEL
                     DERPMLMFDKIKDYPAGFRLVSLAFANYKRTALAFGIAPGKTKLEVVRLLARKMKSGN
                     SIPPAEVKTSPLMENVMQGPQVDLLKFPAPRFHDGDGGRYIGTGDCLINADPDSGFIN
                     AGTYRIQLHEKNLLGLWMSPGQDGRLICAKYWQQGKSCPVVATFGSDPLLFTLAHTKL
                     AYGKSELDYAGGLMGRPLEFIRGPVTGLPIPAGAEIAIEGEIPPPSEEARAEGPFGEW
                     PGYYSGGTIGTGEAQPVIRVKAIYHRNDPILEEEAPLWTGAMKIDGNPTAGILWDQLE
                     AAGVQNVVGVYNHSPYMTVVAIEQKFAGHAKQAGLAAVSCAASARNGRYVVVVDDDID
                     PTNMKEVLWAMMTRVDPPTNIDIVDGAWSTPLDPRMPPDKRESKDHTNGRAIFYAVRP
                     FHWKDKFPKVSRSSRELREQVIKKFRDVIPFPGA"
     gene            4793..5902
                     /locus_tag="VE170_09185"
     CDS             4793..5902
                     /locus_tag="VE170_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15650.1"
                     /translation="MNHLTDKLFSLLMAVSLLANPVLAAEGHGEWNQTLAAARKEGIV
                     VVGIPASTELRTAIGQKFKDKFGISIELLAARGPENVTRIITEYNAGVRYYDVLVAGG
                     ATPLSMVAMGAADDFNGYMILPEVKDPKNWWGGHIWEDNVSTKKHVYAFLCYTSETWW
                     YNSSQAEPSEIRSFDDLLNPKWKGRIGLLDPRSPGSGQNTWSFIWKTKGEEFLTKLAQ
                     QDLLINQNLRQLADGLAKGKLAFTLGLSHYTYDPFIKAGLPVKPVPRVREGAHANNGS
                     GVVTVIKNPPHPNAAKIFVNWLLSREGQELYGKAMTQGTRRLDVSTTWLKEAGSQGCK
                     DVMTVDDYLRVETHLESSVKKIRTPAIALATKLLK"
     gene            5929..7206
                     /locus_tag="VE170_09190"
     CDS             5929..7206
                     /locus_tag="VE170_09190"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15651.1"
                     /translation="MITNEENQLLTQTNRGAPAGELLRRYWQPVALVSELPPGGAPLP
                     VRILGEDLALFRDDQGRVGLLGLHCSHRGTDLSYGRIEDGGLRCLYHGWLYDIRGRCL
                     QQPGEPGGGANRDAIRHLAYPCQEKGGVIFTYMGPGEPPLLPNYEFLNQPAGHFCVYK
                     VFHECNYLQANEGNIDPVHLSFLHRFLEHKEERYTGVRGSDESHYNLIAKARPPKLDV
                     EITDFGVRIYSVRQMGDDQIYLRVSNFVLPNLSTFPGQTGGQGYGINWHVPIDDTHHW
                     KYCFFFNREVPLDQAVIDKSLAEVDEHYRPVRNQSNHFMQDRQSMQTQTYTGMGSGFQ
                     THDAFATGSQGAVQDRTRENIVSSDIAIVAARKLMEKAIRDIQAGGEPPHVMRKPEQN
                     NLSHLLVISDLVSGVSDWKGYTRAMEQEARDRQ"
     gene            complement(7472..7783)
                     /locus_tag="VE170_09195"
     CDS             complement(7472..7783)
                     /locus_tag="VE170_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15652.1"
                     /translation="MSKYLMNKLIHLVNMDEKAEEKYCANPRGFVEDWEKTQRLKLTP
                     EEREALASKDYGKLYALGAHPFTLWSFTEAVWIHEMARPELVADYKAKAAKAGYPNFK
                     T"
BASE COUNT         1778 a         2152 c         2372 g         1617 t
ORIGIN      
        1 cgcgcggcgg attatgacat cgtttatttg cagttctgcg gccgctcgaa gatcgagagc
       61 gaaggcggcg tcatcgaagt cgagcccgga gagatcgccc tggttccttc agcgattgcc
      121 catcgcagca ccggcaccga gcagtgcttg cgcgtgcggg tggcgaccca ggaagcgatg
      181 actgaaggcg tcgatccaga gaagccgctg acgcaaaata aattctccgt caaaccgtcg
      241 gaaccgattg gcgccgccaa tggccacaat gcggacggcg ctggcgacgg aacggcgctg
      301 gagcatatca gcttctggga cccacagtcg gatctatggg tcgagcgcga ggtcgcagcg
      361 ttgatcggct gcaaacgcga aggcggccgg ccggtgcgca agttgagcgc gttcaattat
      421 ttcaccggca tgactggcaa gggcggtgcg cgcgcgccga ttctttacaa cggcaaggag
      481 ttccgcgtcg acgtttacaa tctagaagaa gagcagaacg gttttcatcg tggctgcgac
      541 gaagatgaaa tttggtttca gttccgcggc cattcgctca atcataccga gtggggcgcc
      601 atggagctgg acgccggcca aatgtcctac atcccgcgcg gcgtcgcgca tcgcatcacc
      661 ggcagcccgg gatttctccg tatggtgctg tacagccgca aattggtgca tcccaaggcg
      721 ttcaacaata tttcggagcg cgcgacccgt ttcgacgtgc aatagacttg cttcgtatga
      781 aaaatatcca tgaagagcaa gaaatacgga atatttcgct ctaagttttc gtgcgctttg
      841 ggagcgcact ccgacaatga ccaagcagaa atggagacgg aatgatcggc ggtaaaccca
      901 aaattagcga tctcgtcatt ggcgcgcgca atatgattga agccgcggat attcggcccg
      961 gggattccgt gttgatgttg gccgaccggt cgagcgacgg cgatgccgtc gacgcgctgg
     1021 ctgcggcgtt gcgtttacat ggcgcgacgc cgatggagat gatcgtcgag ccgatcctcc
     1081 ggtatggcga tgttccggaa gcagcgttgc gcgcgatgga agcggtcgac gtggcgatct
     1141 gggtgtggcc ggtatttctc aacggatcga ctaattatcg cgagcgtctg cgcacgccca
     1201 aggaaggcga tgccggcgct ccgacggagc gggtgaaacc ttacaagatt tacttcgaag
     1261 gctggccggg cctgctcgcc accgactacg cgcgctttcc caacgagttg ctttggacca
     1321 tcgcgcgcaa ggtgaaagcg attgtcgcgg cgggctcgaa ggttacgatc agcggaccaa
     1381 acggcgccaa gctcgtcgcc gagtacgaca acaaaagaat ctacgccatg cagaccaagg
     1441 cgggtgaacc ggcggggcgc tgtcattttc cctggggccg ctgcggcgtc tacaacggct
     1501 ccggcacggc caacggaact gtgcagctcg attgcgccca gagcgtgccg gggatgctgc
     1561 gcgaaccgat ggtgtggacg atcaaagacg ccaaagtcgt cggcgtcgaa ggcggcggcg
     1621 atatcggcgc ggagtgcaaa cgcttgttca aggcatttcc cggcagcgat tttttcaccg
     1681 aagtgatgtt cggctaccat cccaaggctt ccactgaacg gggcattcag gatccgatgc
     1741 attgggaaat tctcagccgt tatccctggg tgggtttggg cacgtcgcga aaagaaatgg
     1801 tctaccgcca tgtcgacggc gggtgtatgg agtccaatct agccatcgat gggcgggttc
     1861 tggtggactt cggcggtaag ctcacgattt tggacgacgc tgaaattcgc gccgcggcgg
     1921 cacgctacgg cgatcctgat tttctcttgt cgaccctgtc ccatgaaggc acggcgctgg
     1981 gcggactctg gtaagcctga gccgaaggtg gaaatgcatg aatgattcgc cgcagagaac
     2041 gcaaaggata caaaaaatat ttcagatttg aaaatcgccc ccttggttgc gtagacgctt
     2101 agcgatgcaa tgcaatgaaa gtagcaagat gaagcgatta ggcttagtcg tcctggttag
     2161 tctaatttcg ctcgctaacc accccggctt tattctcgct cagcagccgt ggcaggcgga
     2221 gtgggacaag acggtggcgg cggcgaagaa ggaaggcaag gtcgttgtcg ggctgccgcc
     2281 gagcgccgaa ctgcggcgcg agcttgagcc ggcatttaag gcgcgcttcg gattcgagat
     2341 ggaaatcttt tccgccaccg ggccgcagat cgccaatcgc atcgtcaccg aaggcaaagc
     2401 cggcgtgcgc tatttcgatt cgttcatttt cggctcctgc accggtgtgc cgttgatcaa
     2461 gtcggggata ttcgatccga tcgagcccta catgattctc cccgaggtga aggagcctaa
     2521 gcaatggtgg agcggccatg tcttcatgga caacgtcagc ggcaccaagc ttttctattc
     2581 cttcatctca accaagagta ccgaaggcta ttggtacaac acgtcgctcg caaaagccga
     2641 ggagtttcgc tcactagacg atctgcttaa cccaaaatgg aagggcaaga tcggtttgag
     2701 cgacccgcgt gtggcggggt cgggattgtc ggtctggtcc tatctatggg atctgaaggg
     2761 tgaagagttt ttaaaaaagc ttgtgcagca ggatctcttc gtcacgcaaa acctccggca
     2821 gctcgccgag gccctagcca agggcaaact ggcgatcacg ctgggcatcg gcttggcgca
     2881 gaccgaaccc ttcgtcaagg caggcttgcc gatcaaagat cttcccgata ccagcgaagg
     2941 taatccgtcg agcaacggct acggcacact ggcagtggtc aaaaattcgc cccaccccaa
     3001 tgcgacgaag gttttcgtca actggctact gagcaaagaa gggcaggaaa tctacagccg
     3061 ggtgctgttg catggcacgc gccggcttga cgtcgacacc aagtggatgg cgaagcaagg
     3121 cgtagaagcg tcgaaggaca ccttgagccc gaaggataat gagaaaatgc gcaactatct
     3181 cgaagacaaa tgcgttgagg tcaggttgaa agcgcagaaa ttcgcggaga aagtgttgca
     3241 ataatgcttg gatgaagttc aaaaacgtca agccgttcga atcgttcgaa gtcggaggat
     3301 ggatttgagc aggcgataat tctgcgagca ccctgatgga cgaaattccc tataaagatt
     3361 ttcgcggctt catcgaagcc gccaagaatg tcagcgcatg gcgcttgatc gaaggcgccg
     3421 attggaacaa cgagattggc gcgttgatcg aggcgacggc ggagctcgat gagcggccaa
     3481 tgctgatgtt cgataagatc aaggattatc cagcgggatt ccgtctagtc agcctggctt
     3541 tcgccaatta taaacgtacc gccttggcat tcgggattgc ccccggaaaa accaagctcg
     3601 aggtcgtgcg cttgctcgcg cgcaagatga agtccggcaa ctccattcca cccgcggaag
     3661 tgaaaacttc gccgctgatg gaaaacgtca tgcagggtcc tcaggtggat ctgctgaaat
     3721 ttcccgcgcc ccgtttccac gatggtgatg gcgggcgcta tatcggtacg ggtgattgtc
     3781 tgatcaatgc cgaccccgat agcggcttca ttaacgccgg cacctaccgg attcagctcc
     3841 acgagaagaa tctgctcggc ctgtggatga gtccgggaca ggacggccgg ctgatttgcg
     3901 ccaagtattg gcagcagggc aagagctgcc cggtggtcgc gacctttggc agcgatccgc
     3961 tgctattcac tctggcgcat acgaaactcg catatggtaa atcggaactc gattacgccg
     4021 gtggattgat gggccggccg ttggaattta tccgcggccc tgtgacaggc ttgccgattc
     4081 ccgccggcgc ggaaatcgcc attgaaggcg agattccgcc gccgagcgag gaggcgcgcg
     4141 ccgaagggcc gtttggcgaa tggccgggtt actattccgg cggcaccatc ggcaccggcg
     4201 aagcgcagcc ggtgattcgc gtcaaggcga tctatcatcg caacgatccg atcctcgaag
     4261 aggaagcgcc gctctggacc ggcgcgatga agatcgatgg caatccgacg gccggcattc
     4321 tctgggatca gctcgaagcc gccggcgtcc aaaacgtcgt cggggtttac aatcactcgc
     4381 cctacatgac cgtggttgcc atcgagcaga aattcgccgg ccacgccaag caagccggcc
     4441 tcgccgctgt aagctgcgcg gcgtcggcgc gcaacggccg ctacgtggtc gtggtcgacg
     4501 acgacatcga tccgaccaat atgaaagaag tgctgtgggc gatgatgacg cgggtcgatc
     4561 cgccgaccaa catcgacatc gtcgatggcg cctggtcgac gccgctcgat ccgcgcatgc
     4621 cgccggacaa gcgtgagtcg aaagaccaca ccaacggccg ggcgatcttc tacgccgtgc
     4681 ggccgttcca ttggaaggat aaatttccta aagtcagccg gtcaagccgc gagttgcgcg
     4741 agcaagtgat caaaaaattt cgcgatgtga ttccatttcc tggagcgtaa gtgtgaatca
     4801 tcttaccgac aaattattct cgctgctcat ggcggtttcg ctgcttgcga atccagtgct
     4861 tgccgccgag ggacacggcg aatggaatca aaccctggcg gcggcgcgca aagagggcat
     4921 cgttgtcgtc ggcattcccg ccagtaccga gctgcgcacg gcgatcgggc agaaattcaa
     4981 agacaagttc ggtatctcca ttgaactttt ggcagcgcgc gggccggaga acgtgacgcg
     5041 cattatcacc gaatacaatg ccggcgtgcg ctattacgac gtgctcgtgg ccggcggcgc
     5101 gacgccgctg tcgatggtcg caatgggcgc cgccgacgac ttcaatgggt acatgatttt
     5161 gcctgaagtg aaggatccca agaactggtg gggtggccat atctgggaag acaacgttag
     5221 taccaagaaa catgtctacg cctttctctg ttacacgagc gagacctggt ggtacaacag
     5281 cagccaggcg gaacccagtg agatccgctc gtttgatgat ctattgaatc ccaaatggaa
     5341 aggacgcatc ggtttgctcg atccccgcag ccctggctcg gggcaaaaca cctggtcgtt
     5401 tatctggaaa actaaaggcg aagagtttct caccaagctc gcgcagcaag atttgctgat
     5461 caatcagaac ttgcgccagc tcgccgatgg cttggcgaaa ggcaagctcg ctttcactct
     5521 gggcttgagc cactacactt acgatccgtt tatcaaggcc ggactgccgg tgaagccggt
     5581 gccgcgcgtc agagaaggcg cccatgccaa caacggcagc ggcgtggtga cggtgataaa
     5641 aaatccaccc catccaaatg cggcgaaaat cttcgtcaac tggctactca gtagagaagg
     5701 ccaggagctt tacggcaaag cgatgaccca gggcacgcgg cgcctcgacg tgagcacgac
     5761 gtggttgaag gaggcaggca gccagggttg caaagacgtc atgacggtgg acgactacct
     5821 acgcgtggaa acccatctgg aatcgtcggt gaagaaaatt agaacgccgg caatcgcgtt
     5881 ggcgaccaag cttttgaaat aaacataagg aaagccgaaa atagcgccat gatcaccaat
     5941 gaagaaaatc aattgttgac ccaaaccaac cgcggcgcgc cggcggggga gctgttgcgg
     6001 cgttattggc agccggtagc gctggtttcc gagttgcccc caggcggcgc tcctctgcct
     6061 gtgcgaattc tcggcgagga cttggcgctg tttcgcgacg atcaaggccg ggttggcttg
     6121 ctcggtttgc attgctccca tcgtggcact gatttgagct atggccgcat cgaggacggc
     6181 gggctacgtt gtctctacca tggttggctc tacgatattc gcggccgctg cttgcagcaa
     6241 ccgggcgaac caggcggtgg cgccaatcgt gatgcgatcc gtcatctcgc ttatccttgc
     6301 caggaaaagg gcggcgttat tttcacctac atgggaccgg gcgaaccgcc gctgttgccc
     6361 aattatgagt ttttgaatca accggcaggg cacttttgcg tttacaaagt ttttcacgag
     6421 tgcaattact tgcaggccaa cgagggcaac atcgatccgg tgcacttgtc gtttctgcat
     6481 cgctttcttg agcacaagga agaacgctac accggcgtgc gcggctccga cgagtcccat
     6541 tataacttga tcgccaaagc tcggccgccg aagctggatg tggaaatcac tgatttcggc
     6601 gtgcgcatct attcagtccg ccagatgggc gacgatcaaa tttatcttcg ggtgtcgaat
     6661 ttcgttctgc ccaatctaag cacgtttccc ggacaaaccg gcgggcaagg ctatggtatc
     6721 aactggcatg tgccgatcga cgatacccat cattggaaat attgtttctt cttcaaccgc
     6781 gaagttcccc tcgaccaggc ggtgatcgac aaatccctgg cggaagtaga cgagcattac
     6841 cggccggtgc gcaaccagtc gaaccatttt atgcaggacc gccagtcgat gcagacgcaa
     6901 acttataccg gcatgggcag cggctttcag acccacgatg cctttgccac cggcagccag
     6961 ggcgcggtgc aagaccgcac gcgggaaaac atcgtctcct ccgatattgc catcgtggcg
     7021 gcgcgcaagc tgatggagaa agcgatccgc gacattcaag cgggcggcga gccgccccat
     7081 gtgatgcgca agcccgagca gaataattta tcgcacctgt tggtgatttc ggatttggtc
     7141 agcggggtca gcgattggaa gggttatacg cgcgccatgg agcaggaagc gcgcgacagg
     7201 caataggagt atgaacaacc accgaagaca gagaattagc cgcaaagaac gcaaggttcg
     7261 caaaaaggaa tcggatttgt aggggcgacc ggctggtcac ccttcgttac cgcacgccgc
     7321 gagaatggtt gcgcaagccg cgaagacttt caacaaaatt aagagggggc aaattcgaac
     7381 taagagaatt taaccggcaa agggcgcaaa aaaagcggct gcagcatgct gctcccctac
     7441 gaatgcccgc aatgtggcgc acggctgctt ctcaggtctt gaaattcgga tatcccgcct
     7501 tggcagcctt ggctttgtag tcagccacca gttcgggtcg cgccatctcg tgtatccaga
     7561 ccgcttcggt gaaactccat agagtgaacg gatgggcgcc gagagcgtag agcttgccat
     7621 agtccttgct tgccagcgcc tcgcgctctt ccggtgtcag ttttagcctc tgggttttct
     7681 cccagtcttc gacgaacccg cgcgggttgg cgcaatactt ttcttcggcc ttctcatcca
     7741 tgttgacgag gtggatcagt ttgttcatca aatatttgct cataaagact ccctattgat
     7801 tttcatgcta actatcaacc gcgcgattgg cgtccggtcg aagttgcgcg tttggtgatt
     7861 ccttgcgtaa tctcggtgat ctgaagcaat gctcgtagag cgtcgtttac cgctgcgga
//
LOCUS       DASQKS010000354         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_176791, whole genome shotgun sequence.
ACCESSION   DASQKS010000354 DASQKS010000000
VERSION     DASQKS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176791"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VE170_09200"
     CDS             <1..663
                     /locus_tag="VE170_09200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15653.1"
                     /translation="LQRCAKAGLKGSLIWQAPHPDLPLYSEHYNKFWAASQDLDMPVH
                     LHILTGHGYHKQTVFGNKRTGVEAYRGSVNLKLKEVIDDVFELIFYGVLERYPKMKIV
                     SVENEIGWMPFMMQQWDYYYNRFKKANPPPFTKNPSEYFKTQIFGTFFRDTVAGHNFQ
                     WWGQDNCMWSNDYPHGNSTWPESKKFIDRDLGHLSADIRKKLVYTNVKELYDIDIPHP
                     VH"
     gene            774..2522
                     /locus_tag="VE170_09205"
     CDS             774..2522
                     /locus_tag="VE170_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ15654.1"
                     /translation="MPARFGSDLVVDLMKLLGIEAVALNPGSSFRGIHDSLVNYESGT
                     KPNPEMILCCHEEIAVAVAHGYFKATGKIMPAIVHNVVGLQHASMAIYNAWCDRTPVM
                     VMGGTGPMNSSKRRPWIDWIHTAQVQGNLVRDFVKWDDQPFGVEAIPSSMMRGYRIAM
                     SDPHGPVYLCFDVSDQEAPIDKEIPLPDPRRFRPAAPLQAETESIKEAARLLASAQNP
                     VIVADYVGRNHDAVLSLVQLADLLSIPVIDLGARLNFPNTHALNLTGKNQDLIANADV
                     IMGLDVTDLYGALVKQDPATHAAVPATKPGCKVIHVSLADLAVRSLTSDFQELAPVDL
                     PIVANTKVFLPALIEELQRQGKFSKGVVEERRKGLAAQHDEMHGRWEAILKRRWDEKP
                     IAPPRLAYEVWQALKNEDWLLVAGAFRGWPSRLWTWDKPGLFLGGYGGGGLGYGPAAS
                     VGASLPYRGTGKICVNLQRDGEMLYTPSAMWTAANTGVPLLTVMTNNRVYYNDVEHQE
                     NVAITRGRPVENKLVGMEMAKPAVDFANLARSFSIHGEGPITEPEQIRPAVERAIKVI
                     KTEKRPALVDVYMQMI"
     gene            2587..3591
                     /locus_tag="VE170_09210"
     CDS             2587..3591
                     /locus_tag="VE170_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15655.1"
                     /translation="MIRFRLISAFAAVNLILALAAAGFAQNKPLKKIRVGVPSVGMGN
                     IIIFITKEGKLFEKYGLDAEVITVMGSGIGSKALISGNLDIIPIATPTVIAANLAGAD
                     MAILAHTMPAVVHALMVRPDIKRPEDLKGKKIGVSSLGSLTDFLVRSIAKKKGLNPDR
                     DFTLITTGGSDTERVMALKAGSVEASAVSHPGYGVARKMGFSMLWDSAKELDYPWMEI
                     TTRRAAIKSDRDLITQYMKAHLEGIALFKTNRDFSIKVIKKVMKMTDDELVNESYDIF
                     SKMFLPTPYPNHPGMKISFDYVAMTRNDVWSHKPEEFTDPSFVAELDKSGFIKSLYPK
                     "
     gene            complement(3593..3730)
                     /locus_tag="VE170_09215"
     CDS             complement(3593..3730)
                     /locus_tag="VE170_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15656.1"
                     /translation="MTAGIAATGCQSWRASQKISSPKLKKGLSEYAVLLRLPFNTVMG
                     R"
     gene            complement(3773..>3904)
                     /locus_tag="VE170_09220"
     CDS             complement(3773..>3904)
                     /locus_tag="VE170_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HYJ15657.1"
                     /translation="PCSGHGFKHSAAIGEALAEQVIDGKSKIDMSSFSVKRFSHLLQ"
BASE COUNT          868 a         1078 c         1123 g          835 t
ORIGIN      
        1 ctgcagcgct gcgccaaggc aggcctgaaa ggctcgctga tttggcaggc accccatccc
       61 gatctgccgt tatactccga gcactacaac aaattctggg cggcgtcgca ggatctcgac
      121 atgccggtgc atctgcatat cctcaccggc cacggctacc acaagcagac ggtgttcggt
      181 aataagcgca ccggcgtcga agcgtaccgc ggcagcgtga atcttaagct taaagaagtc
      241 atcgacgacg tcttcgagct gattttctac ggcgtgctcg agcgttatcc caaaatgaaa
      301 atcgtcagcg ttgaaaacga gatcggctgg atgccgttca tgatgcagca gtgggactac
      361 tattataatc gcttcaagaa agcgaacccg ccgccgttca ctaaaaaccc gagtgaatat
      421 ttcaagacgc agattttcgg cacattcttc cgcgacactg tggcgggcca taacttccag
      481 tggtggggcc aggataactg catgtggtcc aacgactatc cccacggcaa ctcgacctgg
      541 ccggagtcga aaaaatttat cgaccgcgac ttgggccatc tctcggcgga tatccgcaag
      601 aaattggtct acacgaacgt gaaggagctt tacgatatcg acatcccaca tcccgtccat
      661 taggcggtgg tcgcggtgac gaaaattggc gcgctctcgg accagttcag gtctggcagc
      721 gcgccatttt tttgccggtt tcgattagat tgaattcgtc tccaggagga tttatgcctg
      781 ctcgtttcgg atctgatttg gtagtcgatt tgatgaagct attgggcatc gaagcagtgg
      841 cgctcaaccc tggctcgagc tttcgcggca ttcacgactc gctggtcaat tacgaaagcg
      901 gcaccaagcc caatcccgaa atgattctct gctgtcatga ggagatcgcc gtggcggtcg
      961 cccacggtta cttcaaagcg accggcaaga tcatgccggc gatcgtgcat aacgtcgtcg
     1021 gtctgcaaca tgcctcgatg gcgatctata acgcttggtg cgatcgcacg ccggtgatgg
     1081 tcatgggcgg cacagggccg atgaattcga gcaagcggcg accgtggatc gactggatac
     1141 acacggctca ggtccagggc aatctcgtgc gcgatttcgt caagtgggat gaccaaccgt
     1201 tcggcgtcga ggcgattccg tcgtcgatga tgcgcggcta ccgcatcgcc atgagcgatc
     1261 cccatgggcc ggtttacctt tgtttcgatg tcagcgatca ggaggcgccc atcgacaagg
     1321 aaatccctct accggatccg cgccgctttc gtccggccgc gccgctgcag gcggaaacgg
     1381 aatcgatcaa agaagccgcg cgattgctag catcggcgca aaatccggtg atcgtcgccg
     1441 attacgtcgg ccgcaatcac gatgcggtgt taagcctggt gcagcttgcc gatctgctgt
     1501 cgataccggt gatcgatctc ggcgcccggc tcaatttccc caacacccac gcgctcaatc
     1561 tcaccggcaa gaatcaggat ctcatcgcca acgcggatgt catcatgggg cttgatgtca
     1621 ccgatctgta cggcgcgctg gtaaaacagg atccggcgac ccatgcggcg gtgccggcga
     1681 ccaagcccgg ctgtaaagtg attcatgtca gtttggccga tctcgcggtg cgcagtttga
     1741 ccagcgactt tcaagagctg gcgccggtgg atttgccgat cgtcgccaat accaaagtat
     1801 tcctgccggc cttgatcgag gagctacagc ggcagggaaa gttttccaag ggcgtcgtcg
     1861 aagagcggcg caaggggctg gccgcgcagc atgatgagat gcacggacgc tgggaagcta
     1921 ttctaaaaag gcgctgggac gaaaagccca tcgcgccgcc gcgcttggcc tacgaagtct
     1981 ggcaggcgct caaaaacgag gactggttgc tggtcgccgg agcatttcgt ggctggccca
     2041 gccggctgtg gacctgggat aaacccggcc tgtttctcgg gggctacggc ggcggcggtt
     2101 tgggttacgg gccggcggcg tcagtcggcg catcgctgcc ctatcgaggc accggcaaga
     2161 tttgcgtcaa tctgcagcgc gacggcgaga tgctatacac ccccagtgcg atgtggacgg
     2221 ccgccaatac cggcgtgccg ctgctgaccg tgatgaccaa caatcgggtt tactataatg
     2281 acgtggagca tcaggaaaac gtcgcgataa cgcgcggccg tccggtggag aacaaacttg
     2341 tcggcatgga gatggccaag ccggcggtgg atttcgccaa cctggcgcgc tccttcagta
     2401 ttcacggcga gggtccgatc accgaaccgg agcagattcg tccggcagtg gagcgggcga
     2461 tcaaagtcat caaaacggaa aagcggccgg cgctggtcga cgtctatatg cagatgattt
     2521 gaaccaaaga gttccagtgt tccaaaggtt ccgaacgttc taattgttcg aattcgaggt
     2581 gaagccatga ttcgctttag gttaatttcc gcgtttgctg ccgtgaattt gattttggcg
     2641 ctggctgccg cagggtttgc gcaaaacaaa cccttgaaaa aaatccgcgt cggtgtgccg
     2701 tccgttggca tgggcaacat catcatattt atcaccaaag aaggtaagct gttcgagaag
     2761 tacgggctcg acgccgaggt gatcaccgtc atggggtcag ggatcggctc gaaggcgttg
     2821 atcagcggca acttggatat aattcccatc gccacgccga ccgttatcgc cgccaatctg
     2881 gcaggtgccg acatggccat tttggcgcac accatgcccg ccgtagttca tgctttgatg
     2941 gttcggcccg atatcaaacg tccggaggat ttgaagggta aaaaaatcgg cgtgtcgagt
     3001 ttgggttcgc tgacggattt tttggtgcga tcgattgcca agaagaaagg gctcaacccc
     3061 gatcgcgatt ttactctaat caccaccggg ggcagcgata ccgagcgcgt aatggcgctc
     3121 aaggcgggtt cagtcgaagc ctccgcggtt tcccatccgg ggtatggagt ggcgcgcaag
     3181 atgggcttta gcatgctctg ggattccgcc aaggaactcg actatccatg gatggaaatc
     3241 accacgcgcc gcgccgcgat taaaagcgat cgcgacctga tcacgcaata catgaaagcc
     3301 catctcgaag gcatcgcgct gttcaaaacc aaccgtgact tcagcatcaa agtcatcaag
     3361 aaggtcatga aaatgacgga cgatgagctg gtcaacgaat cttacgacat cttttcaaag
     3421 atgtttctgc cgacgccgta ccccaatcat ccggggatga aaatcagctt cgactacgtc
     3481 gcgatgacgc gcaacgatgt ctggagtcac aagcccgaag agttcaccga tccgagtttc
     3541 gtcgccgagc tcgacaagag cggctttatc aagtcgcttt acccgaaatg atttatcttc
     3601 ccatgacggt gttgaacggc aggcgcaaaa gaacagcgta ctcagatagc ccttttttca
     3661 gcttcggcga acttattttt tggctggctc gccagctctg acaacccgtc gcggcaatgc
     3721 cggcggtcat gtccatctcc gcatgagcgc gatatctaga tagcgatgga gcctattgaa
     3781 gcaggtggct aaatcgctta acgctgaaac tgctcatatc gatcttgctt ttgccgtcga
     3841 tgacttgctc cgcgagcgct tctccgatcg ccgccgagtg tttgaatcca tggcctgaac
     3901 aggg
//
LOCUS       DASQKS010000355         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4803163, whole genome shotgun sequence.
ACCESSION   DASQKS010000355 DASQKS010000000
VERSION     DASQKS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4803163"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1306
                     /locus_tag="VE170_09225"
     CDS             275..1306
                     /locus_tag="VE170_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15658.1"
                     /translation="MKLLGLLCVLFSFSHDYATAQTPYFQGKTVRIVVGYPTASAHDH
                     WARLIAPQLTKHIPGNPSTIVQNMPGAGSLTATNYVYTVAKPDGLTLGINNAALYFEQ
                     LLKKKEVQFDWAKFTWVGSTTPTSPLLYMWANTPYKSIHDVRTASVAPKCGVTGTGNT
                     GYYLPKLLEETIGTKFQLVLGYEGGTSIELAVERGEVQCRAFTVQVFFGREPFHTWRS
                     KNQVRVLVQGGKKRDPRLPDTPLISELMDQYKVSEAHRRLVTVMMGSGEFGSAPMFAP
                     PGTSPELTKILRAGYATALTSPELIADAKKQGLEPELISAEEIEALAKEVLTQPADVI
                     ASMKKVMGE"
     gene            complement(1411..2400)
                     /locus_tag="VE170_09230"
     CDS             complement(1411..2400)
                     /locus_tag="VE170_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15659.1"
                     /translation="MRVQRIAVLGFLLALLPLHNAAGADKLVGIHSALAISQSLPWIA
                     RDAGIFRKHNLDFDLVLMRSAGTAAAAMIGGETDVGIIGGIGILSAYVQGAQDFVIVG
                     AVKNILTHSIVAKPEIKRPEDLKGKSIGVNRLGSNNHYFAIQVLQRIGLDPAKDVIFR
                     QTGGDAADLIALLNGSVDASAMLTYGQNAVAQGFHYLIYGPDMRIPYAAAAFVSRRSV
                     IARKPQVIGQFMRAMAEASKIFHSDKETTLKILAKYLRVSDRKVLETSYNNELKAMEP
                     RFDMRPEALQAILDELAISDPRAKKVQPQQLIDRRYLEDMEKSGFFDSVWGKR"
     gene            2645..3655
                     /locus_tag="VE170_09235"
     CDS             2645..3655
                     /locus_tag="VE170_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15660.1"
                     /translation="MTKLRWIPLFMLLLCSPLGALAAAAAPVRFNIGAASASSSMLSL
                     WAAQEQGLFAKHGIDPQLILIRGGSTLVASLVTGEIHVAFTSGVSVLGAAAQGIDVKM
                     LTSISSRVSWKLIANPQIKTAAELRGKRVGVQSIVGSTWMYAMLALEQLGLEPKRDNI
                     SFLPIGDAVTIGHALEAGRIDAAVLDPPLSRRLTGKGFSQLVDLAQTNASFPGLGVGV
                     TRNYLDRQAANIERVVTALTEGLAFVQLPANKPAVLKILMKHLRVTDAAVAEDGYQDH
                     LSTLNRKPYPSLDGLRSAQKLMAQQNPKVAALKVDDLVDARFVRKLDESGFIDRLYSG
                     QR"
BASE COUNT          783 a         1059 c         1133 g          769 t
ORIGIN      
        1 gaaatagcac ggtcatgaac cgattcctag gcgaagtttg ggccgttgga atggtcgccc
       61 tccaccgtcg gctcgagacg gaaagtccgc aagggcgggt atcgagtggc ggcagcagga
      121 gaaagccggc ccacaaagct ggactccgct tttgcggaaa acaccgaagg gcttcgaatt
      181 ccatttgacg gtgcgattcc agttgctcta tggtttggag cctgggaacg acgcacggct
      241 atcgaaggcg cgagaattcg gcgcgggagg cgatatgaaa ctgcttggcc tactgtgtgt
      301 gctcttctcg ttttcgcacg attacgcgac agctcagact ccctacttcc agggtaagac
      361 agtgagaatt gtcgtcggct atcccaccgc cagtgcccat gaccattggg cgcgcttgat
      421 tgcgccgcag ttgaccaaac atatcccggg aaatccctcc accatcgtgc aaaacatgcc
      481 cggcgccggc tcgttgactg cgaccaatta tgtttacacg gtggccaagc ccgatggttt
      541 gactctcggc atcaacaacg cggcgcttta cttcgaacag ctgctaaaaa agaaggaggt
      601 ccagtttgat tgggcgaagt tcacttgggt cggcagcaca acgccgacga gtccactcct
      661 atacatgtgg gcgaacactc cctacaaatc cattcacgat gtgcgcacgg cgtcggtggc
      721 gccgaagtgc ggcgtcaccg gcacgggcaa caccggctac tatctcccca agttgctcga
      781 agagaccatt ggcacgaagt ttcagctcgt tttgggatac gaaggcggca caagtatcga
      841 gttggccgtg gagcgcggcg aagtgcagtg ccgcgccttt accgtccaag tgttttttgg
      901 ccgcgagccg tttcacacct ggcgcagcaa gaaccaagtg cgcgtgctgg ttcaaggcgg
      961 caagaagcgc gatccgcgct tgcccgacac gccgctgatc agcgaactga tggatcaata
     1021 caaggtctcg gaagcccatc gccgtctggt aaccgtgatg atgggatcgg gtgagttcgg
     1081 cagcgcgccg atgttcgcgc cgccgggaac ttcgccggaa ctgaccaaaa tattgcgcgc
     1141 gggttatgcc acagcgctca ccagccctga gctcattgcc gacgccaaaa agcagggtct
     1201 cgaacctgag ttgatttccg ccgaagagat cgaagcactg gccaaggaag tgttgaccca
     1261 gccggccgat gtgatcgcgt cgatgaaaaa agtcatgggc gagtagggga tgaagaatgg
     1321 agtgttggag tagtggagta ttggagtgtt gggcctcgga attccgaaaa ctcatgactc
     1381 catcactcca gtactccatc actccaaatc ttaccgtttg ccccacacgc tgtcgaagaa
     1441 gccgcttttc tccatgtctt cgagatagcg gcggtcgatg agctgctggg gttgaacttt
     1501 tttggcgcgc gggtcgctga tggcgagctc gtcgaggatc gcttgcagcg cttccgggcg
     1561 catgtcgaat ctcggttcca tcgctttgag ttcgttattg tacgaggttt cgaggacctt
     1621 gcgatcgctg acgcgcaagt acttcgccag gatctttaac gtggtttcct tgtcactgtg
     1681 aaaaatcttc gacgcctcgg ccatcgcgcg catgaactgg ccgatcacct gcggcttgcg
     1741 cgcgatcacg gagcgccggg agacaaacgc cgccgcggcg tagggaatcc gcatatcggg
     1801 gccgtagatc aaatagtgaa aaccctgagc gacggcgttt tgcccgtagg tcagcatcgc
     1861 cgaggcgtcg accgagccgt tcagcagcgc gatcagatcg gcggcatcgc cgccggtctg
     1921 gcgaaaaatc acatccttgg ccgggtccag gccgatacgc tgcaacactt ggatggcgaa
     1981 ataatggttg ttgctgccga ggcggttgac gccgatgctt ttgcctttga gatcttccgg
     2041 ccgcttgatc tccggcttgg cgacgatgct gtgggtcaga atattcttta ccgccccgac
     2101 gatgacgaag tcctgggcgc cttggacata ggcgctcagg atgccgatgc cgccgattat
     2161 cccgacatcc gtctcaccgc cgatcatggc cgcggcagca gtgccggcgg agcgcatgag
     2221 caccagatcg aagtcgagat tatgcttgcg aaagatgccg gcgtcgcggg caatccaggg
     2281 caaggattgc gagatcgcca gggctgagtg aatgccgacc agcttgtcgg cgccggccgc
     2341 gttgtgcaac ggcaaaaggg cgagcaggaa accaagaacg gcaattcttt ggactcgcat
     2401 aggccaggct cccttcgctg gttcgagata gcgcacgctt actcctccgt cgcaccgtcg
     2461 tcaattgaat tctcgcggtt gccggactcc cgctttgaaa actaatcacg cgacaaatca
     2521 gccgagccgg agaagcacac cgcgatggga tcgattcccc ctccgaaaag ggtgttgtaa
     2581 tacgtggcac taccgtcgtt ggtcgcatat gatgcggcga cttccgccag atggacggca
     2641 atcaatgacg aaacttcgct ggattccgct tttcatgttg ctgctttgtt cgccactggg
     2701 tgcgctagct gccgccgcgg cgccggtgcg gttcaatatc ggcgcggcgt cggcgagcag
     2761 cagcatgctc agtttgtggg cggctcagga gcagggactg tttgccaagc atggcatcga
     2821 tccgcagctg attttgattc gcggcggctc gaccctggtg gcgagcttgg tcaccggcga
     2881 gattcacgtg gcgtttacct ccggcgtctc agtactcggc gcggcggcgc agggtatcga
     2941 cgtgaaaatg ctgacatcga tttccagccg cgtcagttgg aagctgatcg ctaatccgca
     3001 gatcaaaacc gcggcagagt tgcgcggcaa gcgtgtcggc gtccagagca tagtcggcag
     3061 cacctggatg tacgcgatgc ttgcgctgga gcaactggga ttggaaccaa aacgcgacaa
     3121 catttctttt ctgccaatcg gcgatgccgt gaccatcggt catgccttag aagccggccg
     3181 catcgacgcc gcggtgctcg acccgcccct cagccggcgg ctcacaggca aaggcttttc
     3241 gcagttggtc gatcttgccc agaccaatgc gagtttccct ggcttgggcg tgggcgtgac
     3301 gcgaaattat ctcgatcgcc aggctgcaaa catcgaaaga gtcgtgaccg cgctgactga
     3361 aggcttggca tttgtccaat taccggcgaa caaaccggca gttcttaaaa ttctcatgaa
     3421 gcatctgcgc gtcacggacg cggcggttgc ggaagacggc tatcaagacc atctgtcgac
     3481 gctcaaccgt aagccctatc cgtccctcga cgggctgcgc agcgcgcaaa agttaatggc
     3541 gcagcagaac cccaaggtcg ccgcgctcaa ggtggacgat ttggtcgatg caaggtttgt
     3601 ccgcaaacta gacgaaagcg gattcatcga tcggctgtac agcgggcagc gctgagggcg
     3661 ttgtcgagtt tagattctga ttttcctccc gcaaggcgca aagacgcaaa ggtgacgggc
     3721 gaggcccgtc atcccgagcg aatg
//
LOCUS       DASQKS010000356         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3127970, whole genome shotgun sequence.
ACCESSION   DASQKS010000356 DASQKS010000000
VERSION     DASQKS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3127970"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1224)
                     /locus_tag="VE170_09240"
     CDS             complement(385..1224)
                     /locus_tag="VE170_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ15661.1"
                     /translation="MTKISSANFPRAIVTKTNPTDTQGPLLFSAASYNIHQGVGSDGK
                     RDDRRIVRVLQSLDAGIIGLQEVDSEVSGDENFQLKYLTGETDLKAVPGTTMRRRGSD
                     FGNVLLTRYKILAVRNVDLSVSRREPRGAIDVDLDIGGAVVRVIVTHLGLSGRERRRQ
                     THMLSALVSERRDRMVVVLGDLNEWHPYGRSLRSMARHFGTSARLRTFPSRMPLFALD
                     RIWVWPEQALVETRVHRLAPARTASDHLPIKAKITTAYAQPLKLEGGAHDLSATLNLD
                     PIP"
     gene            1306..1533
                     /locus_tag="VE170_09245"
     CDS             1306..1533
                     /locus_tag="VE170_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15662.1"
                     /translation="MSTNREGMPKGGPERTDSIKIQITGSENCTVLTSKEGAAFSQQI
                     SQNPDIPLLCGDLVHSGQLEKATALAKELGD"
     gene            1808..>3669
                     /locus_tag="VE170_09250"
     CDS             1808..>3669
                     /locus_tag="VE170_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HYJ15663.1"
                     /translation="MKRLLNFQFLRQRFGTLSLRSHLFALVVVSMLPFLIFAFLVLNL
                     FASREKASLTVGLRETTRALSSALDREFEATKTALESVGTLEDFDSGDLGEVYRKLRR
                     VLMSRPNWRTIVVHDPSGRQLLNLLNPFGEPLPEADIERESFRATLATKQPMPINFHR
                     DPDAGPMVGYRVPVLRGGEIRYVLSVEIAPAVYGEILARQKLPAQAVAMIVDGRNIIV
                     GTSGVQELVGQVAGPLVDSAAPGELDGWFEGADRQGVSTYLAFSRSPTNGWAVSLAIP
                     AAQLEDPLTRSFGALLGAGVIFLVVGILLAVIVETRISDPLDELTRQAGALGRGETVV
                     MGGHSRVTEVENLRRDIGRTAGLLEQRGQERDRAAAALRELNDRLEHRILERTEELEE
                     ANSELRKEIRERHLAEAALRAEHVYLNLLRSTELVINQAGSIEGALNAALEQICHHLG
                     LPVGYCLTLAGAEQGRRISFWNIEAAPERFAALQLALEQAAADSSDPASMVLSSSDVS
                     CCNDLAGQSEPWACAATNAGLISVCTFAVFGGGAPAGALAFFSDRAIELDQRLSTLLA
                     QLAMQLGRAVERQQADEALRLSEERFRSAFDEGPIGIGMVDLEMRYLRVNQVLCG"
BASE COUNT          746 a         1000 c         1093 g          830 t
ORIGIN      
        1 gtatcatttt taagataact tcttcaccat agatttcgct aatctccata ttttttccca
       61 gccattctct tttctcgaga atggcatatg ggttgctcag cgtaaattcg gcgtcagtct
      121 agaaaaaatt tagggggcac cgatttatgt ttaactattc cgtgaaccaa cggtcaagtt
      181 tttggctgcg agtgtttagt aatagtaaag ttaatggggc gccttattgg ggcgacaatt
      241 taccggccta ccaatgggct tggcccaccg gcacttacct gcaacagcgc ttacgtacag
      301 tccgaactgg ggccgccgac ttcacgatac cggcaagcga ggcgccggcc ctgcggcgaa
      361 acgaatcggc ggccacacgc cggtttatgg aatcgggtcg aggtttaatg ttgcactaag
      421 gtcgtgtgcg ccaccttcga gcttcagcgg ttgtgcgtat gcggtggtga ttttcgcttt
      481 aatcggtaga tggtcggagg cggtgcgtgc aggggctagc cggtgcaccc tggtttcgac
      541 gagcgcttgc tcgggccaaa cccagatgcg gtccagagca aacagcggca tccttgaagg
      601 aaaggtgcgc agccttgcgg atgtgccgaa atggcgcgcc atactgcgta gcgaacgccc
      661 ataagggtgc cattcgttaa ggtcgccgag gactacgacc atacggtcgc ggcgctcgga
      721 aacgagagca ctgagcatgt gggtttgccg ccgccgttcg cggccgctta accctaaatg
      781 agtgacaatg acacgtacga cggcgccgcc gatgtccaaa tcgacgtcga tggcgccgcg
      841 cggttcgcgc cgggagacgc tcaaatcaac gtttctcact gccagaatct tatatcgagt
      901 tagcaacacg ttgccaaaat cgctaccgcg acggcgcatc gtcgtaccgg gaaccgcttt
      961 taggtcggtt tccccggtga gatattttag ctgaaaattt tcatccccgc tgacttcgct
     1021 gtcgacttcc tgcaagccga taattcctgc atcgaggctt tgcagcaccc gcacgatacg
     1081 ccggtcatcg cgcttcccgt ccgagccaac cccttgatgg atattgtagg atgccgccga
     1141 aaacagcagc ggtccctgag tatcggtcgg attggttttc gtcacaatcg ctctcgggaa
     1201 attggctgag gatatttttg tcatttctca agccccgatt caacgtgcat tcggcgccgc
     1261 atcagaagag cgtttttttg tagcggagtc cgatcagtta cgaccatgag cactaatcga
     1321 gaggggatgc ccaaaggcgg gccggagcgg accgactcga ttaaaatcca aattaccggg
     1381 tcggagaatt gcactgtgct aacttccaag gaaggtgcag cgttttcgca gcagatttct
     1441 caaaacccag acattccgct gctatgcggc gatttggtac actctggcca actggaaaag
     1501 gcgacggcgc tggcgaaaga attgggcgat tgaaatcccg gtgccggcct cgctggggaa
     1561 tcataagttt gtctgctaga ccgaattatc gaacgagcac tcgattgctg catgataaaa
     1621 gactcttgta accccgcatc gtgattgtca tggttagact accggcccgt ggatatccga
     1681 cctcttgttg ttgaattatg attgaaaaat cgaaaccagg aggtcttgcg ctgtcaagcg
     1741 gcgcaatacg gtatagtcac aggcgcaaat ccggggaaat cccttttctt aactgaagaa
     1801 gcggccgatg aaacgattgc tcaattttca atttctgcgc cagcgtttcg gcaccctttc
     1861 actgcgctcc catctgtttg cgctcgtggt ggtgtcgatg ctgccatttc tgatatttgc
     1921 gtttctggta ttaaatcttt tcgcgagtcg ggagaaagcg tccctgacgg tcggcttgcg
     1981 cgaaacgacg cgggcgttgt cctcggcttt ggatcgtgaa ttcgaagcta ccaaaacggc
     2041 tctcgaatcg gtgggaactc tggaggactt cgacagcggc gacctcggcg aggtctatcg
     2101 caagctccgg cgcgtcctca tgagccgtcc caattggcgc acgatcgtgg tccacgaccc
     2161 ctccggccgt cagcttttga atctgctcaa cccattcggt gaaccgttgc ccgaagccga
     2221 tatcgagcgg gaaagtttcc gggccaccct cgccaccaag caacccatgc cgatcaattt
     2281 tcatcgcgat ccggatgccg gtcccatggt tggatatcgc gtccccgtgt tgcgcggtgg
     2341 cgagatacgc tacgtgctgt cggtggagat tgccccggcg gtgtatggcg aaattctcgc
     2401 gcgccagaag ttgccggccc aggccgtcgc catgatcgtc gatgggcgca atattatcgt
     2461 cggcaccagc ggcgtccagg aactggtcgg ccaggtcgcc ggaccattgg tggacagcgc
     2521 agcgccgggc gagctcgatg gttggtttga aggagccgac cggcaaggcg tctccactta
     2581 cctggcattc agccgttcac caactaatgg ttgggcggtc tcgttggcga ttcccgccgc
     2641 acaactcgag gacccgctaa cgcgttcttt cggggctttg ttaggcgcgg gggtgatctt
     2701 tctggtagtc ggcatcttgc tcgcggtgat cgttgaaacg cgcatcagcg acccgctcga
     2761 tgaattgacg cgccaggccg gcgctctcgg gcgcggcgaa acggtcgtca tgggaggcca
     2821 ttcccgggtg actgaagtag agaatcttag gcgcgatatc ggccgaaccg ccgggctact
     2881 cgagcagcgt ggtcaggagc gcgatcgcgc ggcagctgcg ttgcgcgaat taaatgatcg
     2941 tttggagcat cgcatcctgg agcgcaccga agaattggaa gaagccaaca gcgagctcag
     3001 gaaagagatc cgcgagcgtc atctagcgga agcggcgcta cgcgcagagc atgtttattt
     3061 aaatctgctg cgaagcaccg agctggtgat caatcaggcg gggagtatag aaggcgctct
     3121 gaacgccgcc ctcgagcaaa tttgccatca tctgggattg ccggttggct actgcttgac
     3181 cttggctggg gccgagcagg ggcgacggat ttcgttctgg aatatcgaag cggccccgga
     3241 gcgctttgct gcgctgcagc ttgcgcttga gcaggccgct gccgacagta gtgacccggc
     3301 aagcatggtc ctatcgagca gtgacgtaag ttgctgcaac gatctggccg ggcaatctga
     3361 gccatgggca tgcgcggcga ccaatgcggg actgatatcg gtctgcactt ttgccgtctt
     3421 cggcggcggc gcgccggccg gtgctctggc ttttttttcc gaccgcgcga tcgaattgga
     3481 tcaacggctc tcgacattgt tagcgcagtt ggcgatgcag ctgggccggg ccgtcgagcg
     3541 ccagcaagcc gatgaagcgt tgcggctcag cgaagagcgc tttcgttcgg cctttgatga
     3601 agggccgatc ggcatcggca tggtcgatct cgagatgcgc tatctgcgcg tcaaccaagt
     3661 gctctgtgg
//
LOCUS       DASQKS010000357         5139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3554800, whole genome shotgun sequence.
ACCESSION   DASQKS010000357 DASQKS010000000
VERSION     DASQKS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554800"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2264)
                     /locus_tag="VE170_09255"
     CDS             complement(72..2264)
                     /locus_tag="VE170_09255"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ15664.1"
                     /translation="MGNIANVYDDVRAVEEHIGLGLLFQLRGATTAPAEALVVAIDSE
                     SSERLNVSKNPDRWPRTLHARLIEALNRAGASVIVFDLYFAEPRSPEEDAALAAAIKK
                     AGNVVLAEQLRAKEVTSAGDAMIDDAGHKIVEALKPIEPFSHAAFATAPFVLPRMPVR
                     VSQYWTFQTDAGDTPTFPVLALQLYTLQAYPEIRALLERASPNHAAQLPRDASSAIAS
                     IGAAKFIRDLRQVFRRDVDAAEASAHVAAGSGGDHRPVVALVDLYTGTNRQYINFYGP
                     PRSVTTLPYYQALQIGNDGTDGAPTDLRGKAVFVGLSENLLAERQDSFHTVFSTNKGV
                     FVSGVEIAATAFLNLLTSTSIKPLRPGHEILTLLLWGMSVGIICRLMTPLSAAVAVIL
                     CCSAYMFAAAYQFKANALWFPIVVPLMGQAPLGFVSALLWNYFEINRERKRITRALGY
                     YVPEEVVTRVAKDMIDIRRFGQTVYGTCLFADVAGYTPVSEAMTPRDLSEHMHKYLEA
                     AFEQIHKQGGLVVELKGDSILAVWKGSLGERAFRQKACEGALDMADAIRRFNQSADAA
                     IKLPTRVSLHAGPIYLGNIGAGTHYEYGVTGDTVTTAARLDGLNKYLGTKVLVSDEVM
                     RDLDKLLSREVGVFLLKGKSQPIIAHELICRAVDCVDWQKKACEIFAEARQAFQFRLW
                     DEADEKFLQSDQMFRGDGASQFYRQLCAQYRSSPPPEGWNGTIAMAEK"
     gene            2772..3041
                     /locus_tag="VE170_09260"
     CDS             2772..3041
                     /locus_tag="VE170_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HYJ15665.1"
                     /translation="MADLSEEQKKSYWRYNITLTTILLIIWFVVTYLVSGLWAASLNQ
                     FSFLGFPLGYYMAAQGSLAIFVIEIAVYAYLMNRLDLKYGIREEG"
     gene            3045..4973
                     /locus_tag="VE170_09265"
     CDS             3045..4973
                     /locus_tag="VE170_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HYJ15666.1"
                     /translation="MSLGKIFGLYTVCFLGVTILIGLAETFLGLSPQWIGWIFMGLSL
                     GIYIVIGIITRTSNPDQYYVAGRGVPAVFNGMATGSDWMSAASFISMAGALSAQGFAG
                     LAYVMGWTGGYLLLAVFLGPYLRQFGAYTIPDFLGARYGGNLARIIGVVAAIACSFTY
                     LIAQVTGVGLIVSRFIGLDFQVGVFVGLIGVLFCSVLGGMKSVTWTQVAQYIILIVSY
                     LVPVVYLSYQLFSIPVPELIYGRVLQINNGKAIEITRDSKEKETRALWKKDADEANAK
                     IKVGNLTDAETDKLKAAAKTATQQATAPAASDDAKISRYLTVPSGVGMWNFLALTFCL
                     MVGTAGLPHILTRYYTTPSVRQARTSVAWSLFFIFLLYFTAPAYAAFARYTIYVKLVG
                     TKIAELPRWITLWQPAGLFEVVDKNGDGIVQWADFVVKSTDFVVLSMPEVAGLPFVIT
                     GLVMAGGLAAALSTADGLLLTIANAFSHDLYFNVIDPKASLTKRLTITKILLVVTALV
                     AAWVATFRVAIIVELVAWAFSLAGASFFPALVMGIWWKRANKTGACAGMIAGLAVTAY
                     YMIGSRFYGVSWFGTQTIASAVFGLPVGFLVIWIVSLMTAAPSKEVQELVANVRYPKS
                     GQATTGDPLGREQPAGAR"
BASE COUNT         1051 a         1459 c         1388 g         1241 t
ORIGIN      
        1 cttcagcgta cgcttcttca tgactcgcct gctttcagtc atgcgtttct ttttcatcga
       61 tacgatgccg gttatttttc agccatcgct atcgtgccgt tccagccttc cggaggcggg
      121 ctggagcgat actgggcgca tagttgacga taaaactgcg aagcaccatc gccacgaaac
      181 atttggtccg actgcaggaa tttttcatcg gcttcgtccc aaagccgaaa ctggaaagcc
      241 tgccgcgctt cggcaaatat ctcgcaagct tttttttgcc agtcgacgca gtcgacggcg
      301 cggcagatca attcgtgggc tatgatcggc tgcgacttgc ccttcaagag aaatacgccg
      361 acctcccggc tcagcagttt atcgaggtca cgcatgactt catcggatac caaaactttt
      421 gtcccgagat atttattcaa cccgtctaat cgcgccgccg tggtgaccgt atccccggtc
      481 actccgtatt cataatgcgt ccccgcgccg atgttaccga gatagatcgg cccggcgtga
      541 aggctaaccc gcgtgggcag tttgatcgcg gcgtcggcgg attggttgaa gcggcgtatc
      601 gcgtcggcca tgtcgagagc cccttcgcag gccttttgcc ggaaagctcg ttcgcccaag
      661 ctgcctttcc agacggccag gatcgaatcg cctttgagtt cgacgaccag accgccctgc
      721 ttatgaatct gttcgaatgc cgcctcgaga tatttatgca tatgctcgct tagatcccgc
      781 ggcgtcatcg cttcggaaac cggcgtgtag ccggcgacat cggcgaataa acatgtgcca
      841 taaacggttt gtccgaatcg acgaatgtcg atcatgtctt tcgccacccg ggtgacaact
      901 tcttccggca cataatagcc gagcgctctg gtgattctct tgcgttctct gttgatttcg
      961 aaatagttcc ataagagcgc actgacaaat ccaagaggcg cttggcccat caggggcaca
     1021 acgatcggaa accataaggc gttggctttg aactggtagg cggcagcaaa catataggcg
     1081 gaacagcaca agatcactgc gaccgccgcc gagagaggag tcatcaagcg gcaaataatt
     1141 cccacgctca tgccccaaag caggagcgta aggatttcat gacctgggcg cagcggtttg
     1201 atagaagtgc tcgtcagtaa gttcaaaaat gccgtggccg cgatttcgac gccgctgaca
     1261 aaaacacctt tgtttgtgga aaaaaccgta tggaagctgt cctgtctctc ggcaagcagg
     1321 ttttccgata atccgacaaa gaccgctttg ccgcgcaggt ccgtgggcgc accgtcggtg
     1381 ccatcgttgc cgatctgtaa ggcttgataa tacggcaggg tggtcaccga gcggggcgga
     1441 ccgtagaaat ttatgtattg acgattcgtc ccggtgtata gatcgaccaa tgcgactacc
     1501 ggtctgtgat cgccgccgga accggcggcc acatgcgccg atgcttcggc ggcgtcaaca
     1561 tctctgcgaa atacttgtct caaatccctg atgaattttg ctgcgccaat cgatgcgatc
     1621 gccgaacttg cgtcccgcgg cagctgcgct gcatggttcg ggctcgccct ttccagcaac
     1681 gctcggatct ccggataggc ctggagcgta tagagttgca gcgcgaggac gggaaacgtc
     1741 ggcgtgtccc cggcatcggt ttgaaacgtc caatactggc tcacccgcac cggcatgcgc
     1801 ggcaaaacga atggcgccgt ggcgaaggcg gcatgggaaa acggttcgat cggctttaat
     1861 gcttcgacaa tcttatgccc tgcgtcgtct atcatcgcat cgcctgctga agtgacttct
     1921 ttcgccctca attgttcggc gagcacgaca ttcccggcct tctttatcgc tgcggcgagc
     1981 gccgcgtcct cctccggcga cctgggctcg gcgaaataga gatcgaacac tatgacgctg
     2041 gcgccggctc ggttgagtgc ttcgatcaag cgcgcatgca gggtgcgcgg ccagcgatca
     2101 ggattcttgg agacgttcag acgttcggaa gattcgctgt cgatcgcgac gacaagcgct
     2161 tcggctggag ctgttgtggc gccccttaac tgaaacagca aacccaggcc gatatgttcc
     2221 tcaaccgcgc gaacatcgtc gtaaacattt gcgatgttcc ccaatagccc tagtaaaagg
     2281 ccgaacgcgg cagctttaag caagctcaac aagtgcgtcg ttcccgccca cagtttttac
     2341 ccctcaaaga gcaaccatgc ggccgttggt cgaccgacac ctcgcgacca agctggatag
     2401 cacggtctat agtccaaacc cgtcaagatg cgcaagcaga atgctgttgc tggagaggcc
     2461 cccggcgttg gcttagcacc ggcgcaatct gattaaacag cgtgccatta acccatgtca
     2521 gaatcacgtt gctacgacac cgccataaat accgcttgac accggaatac cgcttttgtt
     2581 acaagcccgc gttactggtt gctctcatgt gtcgggtagc gattctcctg cgctattcat
     2641 gagagccggt aaatatcccg ccctcaagtg gcgctcgatt cactaacgcc gggctcactt
     2701 gaatgaaatt catataggaa cgggttttct ctcgtggcga cagattcaac aattgcatcg
     2761 aggaggtgcg tatggctgat ctctcggagg aacaaaagaa gtcctattgg cgttacaata
     2821 tcacactgac aaccatccta ctgatcatct ggttcgtcgt cacttacttg gtctcgggcc
     2881 tatgggccgc cagtttaaat caattctctt tcttgggttt tccgctcggc tactacatgg
     2941 cggcccaggg ctcgctcgcg atcttcgtca tcgagatcgc cgtatatgct tacttgatga
     3001 acaggctgga tctcaaatac ggcatccgag aggagggttg aatcatgagt ctaggaaaaa
     3061 ttttcggcct ttacaccgtc tgttttctcg gcgtcacgat tttgatcggc ctcgcggaaa
     3121 catttctcgg cctgtcgccg caatggatcg gctggatatt catgggcttg tcgttgggta
     3181 tttacatcgt catcggcatc atcacgcgca cgtcgaaccc ggatcaatac tatgtcgccg
     3241 gccgcggcgt gccggcggtg ttcaacggca tggccaccgg ctcggactgg atgtccgcgg
     3301 cgtcatttat ttcaatggcc ggcgcgttgt ccgcccaagg gtttgccggt cttgcctacg
     3361 tgatgggatg gaccggcggc tacctcttgc ttgccgtatt tttaggaccc tacctgcggc
     3421 aatttggcgc ctacacgatt cccgattttc ttggcgcgcg ttacggtggc aacctggcgc
     3481 gtatcatcgg agtcgtcgcg gcgattgcct gctcgtttac ctacttgatc gctcaggtca
     3541 ccggtgtcgg cttaatcgtc tcgcgtttta tcggcttgga tttccaagtc ggcgtctttg
     3601 tcggtttgat tggcgtgctg ttctgctcag ttctcggcgg catgaagtcg gtcacctgga
     3661 ctcaagtcgc ccagtatatc atcctgatcg tttcgtatct ggtgccggta gtttacctgt
     3721 cgtaccagct tttctcgatt ccggttccag aattgatcta cggacgtgtt ttgcaaatca
     3781 ataatggtaa ggcgatcgag atcacccgtg attcgaagga gaaagaaacc cgcgcgctct
     3841 ggaagaaaga tgccgacgag gcaaatgcca aaatcaaagt cggcaatctc accgatgcag
     3901 aaaccgacaa acttaaggcg gcggcaaaaa ccgccaccca gcaagccaca gcgcccgcag
     3961 cgagtgacga cgccaaaatt agccgatatt taaccgtacc aagcggggtg ggaatgtgga
     4021 actttctcgc tctgactttt tgcttgatgg tcgggacggc aggactgccg catattctga
     4081 cgcgttatta caccacgccg tcggttcgcc aagcgcgtac ctcggtggct tggtcgcttt
     4141 tcttcatatt tctactttat ttcaccgcgc cggcctacgc cgccttcgcc cgttatacga
     4201 tctacgtcaa gctggtcggc accaagattg ccgaattgcc gcgctggatc actctctggc
     4261 agccggcggg gcttttcgaa gtcgtcgaca aaaacggcga tggcatcgtg caatgggccg
     4321 atttcgtcgt caaatcaacg gactttgtcg tcttgtccat gcccgaggtt gccggcttgc
     4381 cgttcgtgat caccggtttg gttatggccg ggggtctagc agccgcgctc tccaccgctg
     4441 acggtttact actgactatc gccaacgcct tttcccatga cttgtatttc aacgtcatcg
     4501 atcccaaggc atctctgacc aagcgcctca cgatcactaa aattctgttg gtagtcaccg
     4561 cgctggttgc cgcttgggta gcgactttcc gagtcgccat catcgtcgag ttggtcgcct
     4621 gggcatttag cttggcgggc gcctcgtttt ttccggcgct agtaatgggt atctggtgga
     4681 aacgggcgaa caaaactggc gcttgcgccg gcatgatcgc aggattggcc gtaaccgcct
     4741 attatatgat cggcagccga ttttacggtg tttcttggtt tggcacgcag accatcgctt
     4801 cggccgtgtt tggcttgccg gtgggcttcc tggtcatctg gatcgtctcg ctgatgaccg
     4861 cagcgccgtc gaaagaagtc caagaactgg tcgccaatgt gcgctatccg aagagcggcc
     4921 aagccactac cggcgatccc ctgggacgcg agcagccggc aggcgcccgc taagataatt
     4981 cttatccacg gtggcgagat cgccctatgt ctcgccggcg tggacggcca gtaaattgat
     5041 cgaaccaacc gcgcaaatcg tttttctact gcgcttcgtc tgcttcatgg cgatttttta
     5101 tctcgctttg cacaaaattg tcgcgcgcat gagccgcaa
//
LOCUS       DASQKS010000358        17140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1449650, whole genome shotgun sequence.
ACCESSION   DASQKS010000358 DASQKS010000000
VERSION     DASQKS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449650"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VE170_09270"
     CDS             complement(<1..174)
                     /locus_tag="VE170_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HYJ15667.1"
                     /translation="MPIFRLVDDLVFPPPDYADPSGLLAVGGDLSSDRLLAAYRLGIF
                     PWYSEDQPILWWSP"
     gene            291..659
                     /locus_tag="VE170_09275"
     CDS             291..659
                     /locus_tag="VE170_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15668.1"
                     /translation="MFKRIDHVALHVADLDRSVAFYERHFGFKDYFRQTASGGLQIAY
                     LRLADTVLELTHHSDGAMAGFHFCLETDNFNETVEQLQRDGVKLVRAPHDTAPREPRE
                     SGWRRVVFAGPDGEQIELRG"
     gene            726..1031
                     /locus_tag="VE170_09280"
     CDS             726..1031
                     /locus_tag="VE170_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15669.1"
                     /translation="MSNENKTIQVTCPCCDALLTIDPILAAVLDHKTPVRPQMAVNLK
                     DAVNQVKGEAGRRDEKFKQFAEAEKNKSKVLEAKFQELFKKAKDEPLTKPLKDIDLD"
     gene            complement(1098..2225)
                     /locus_tag="VE170_09285"
     CDS             complement(1098..2225)
                     /locus_tag="VE170_09285"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYJ15670.1"
                     /translation="MGEELTNKLPRWIFATTILLLGFLLAGCAQLPQLLHLPQSQPQI
                     VFDTGPIADPVLSAPVRVSRSPEENEDPSVILARDGRFYVVWSSKQRGRVDLYISSSA
                     DGRTWSEERRIHDVPAEDYYPSLMQTRDGRFHLVWCQLQRKEGRTDVWYTASRDGRTW
                     DKPQSITNRGIDWAPTIYEDAKGVLRILWSSRRSGSRDIWMTRSLDGGKTWLSPDQVT
                     RSAEEDDFPHALQASSGEHYLVWTRYRRGSAMLELHKDRSSDVVLATSRDAVVWSAPV
                     VSSFGDSDNKFVDILPALFQDPDRGQVFVSWTSGRTGMRQDILARSITSPSSHVFQLT
                     RSDTSDFSAKIVPTRQSGEYLMVWVSSREGKPDIFARRIRL"
     gene            2383..2718
                     /locus_tag="VE170_09290"
     CDS             2383..2718
                     /locus_tag="VE170_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15671.1"
                     /translation="MIAQTSFHGIAALAVIVARRISSLRSSPSNRDSDEHDLLTGLKR
                     APLTIDKQNLPRQLAAIKPMNCHHSMFLIVLVARQTLVSCPAPREKPKQRFGQSTDLS
                     QQLNSMMIT"
     gene            2785..3792
                     /locus_tag="VE170_09295"
     CDS             2785..3792
                     /locus_tag="VE170_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HYJ15672.1"
                     /translation="MLAMVLDHPGEALRHSEISTPDPGPGQLLIQVHACAVCRTDLHI
                     VDGELTQAKLPLILGHEIVGKVAQLGGGVSQFKIGDRVGVPWLGWTCGTCWYCRSDQE
                     NLCDHGKFTGYTIDGGYAEYTVADQRFCLPLPDGYSDAEAAPLLCAGLIGYRSLVKAG
                     KAKRLGLYGFGAAAHIVAQVARYQQRAIYAFTRPGDEAAQQFAITLGAVWAGGSNQTP
                     PEKLDAAIIFAPAGELVPLALKAVGKGGTVVCGGIHMSDIPSFSYSILWEERTLTSVA
                     NLTRRDGAEFMALAARVRVRTAVEAFELQQANEALRRLRDGKIEGAAVLTTAAANAQT
                     V"
     gene            complement(3794..4723)
                     /locus_tag="VE170_09300"
     CDS             complement(3794..4723)
                     /locus_tag="VE170_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15673.1"
                     /translation="MKVRIGIQLGQWPSRDMRPEAVLDLVDYFESLDVDSIWLSDRLA
                     STALTLEPVTFLAYVAGRLRKMKFGTSTLVLPTRHPVVLAKELATLDFLSQGRLFPAI
                     GLGGDESKDLQAVGINKKERAGRADEMIVLMRRLWTEDNVNFAGKYFTVEDVTILPRP
                     WQKNGLPIWIGGRSEAALKRTGRLGDGWLVSSVSPAEVATGIESIRRYAADAGRRVEE
                     DHYGVLIPFYFAAERVKAAELGGRSIRPRSDLPTSEFTAFGTPDHVRARVQAYIAAGA
                     SKFVMRPCGPFNGWREQVEILAREVIAPLQTAD"
     gene            complement(4733..5665)
                     /locus_tag="VE170_09305"
     CDS             complement(4733..5665)
                     /locus_tag="VE170_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15674.1"
                     /translation="MAQTRGLKKVQVGVPAISMGNIIIYFTKEAKIFEKHGLDVEPIA
                     VAGSGIASKALISGSVQISPIATPTVMNSVLSGSDMVILAHTMPGVIQYLMARPDIKR
                     TEDIKGKTIGVTTFGSLNDFLVKYLARQKGLNPDKDFAMLQIGSDADRFIAMQQGRIP
                     MSTLSHPNYIYAQRAGFHVLWDFFKEVDYPWSEIATTRTQIKQDRDLVMRYMRAHIEG
                     IARFKQEPELGKRVIKKMLRLNDDPMAQESWELFAKHRLSAPYPNLKGMKTSIEYLAA
                     TRPEINKYKPEDFVDASFVEELDKSGFIKKLYEK"
     gene            5846..6877
                     /locus_tag="VE170_09310"
     CDS             5846..6877
                     /locus_tag="VE170_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ15675.1"
                     /translation="MLPNLPRENLLELCRRMFVIRHFEENLVGLHDKGVFGGHYHLYV
                     GQEATGVPALACLDADDYLFTTHRNHGHLLARGAEPKKLFAEILGRVDGLNKGKGGSF
                     HSVAPGLGFLHSSGVVAGIIPLSTGTAYASKAQKNGRLTVCLFGDGSLEEGAAPEAFN
                     IASLLKLPVLFLCENNGKYGEGRAVAAAQSGSMAAYPLTDLPKAYKIPAQQIDGIDAG
                     LVHATISEAVEKIRRGEGPQFVEAQTVRYPGSATNWPTVPAPTDTTLAWDVSQVPENA
                     REWYRSADPLLVYVRELVEANQAKREEIAALESQVKSAIAAAVDFAVNSPYPKPEEAE
                     RDFFAKSPL"
     gene            6874..7845
                     /locus_tag="VE170_09315"
     CDS             6874..7845
                     /locus_tag="VE170_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYJ15676.1"
                     /translation="MRELAYYEAKFEALDEIMSGDERVHLINGTFMSLSPHRHVYQNL
                     QKKFKARIFSPPISELGFCGLATGAAMAGLRPIVDLSTGSFIYEAWPQVANEAANAYS
                     MSGGQTRVPVVFHLFHGLRGGGAAQHSGSPQAMLWNVPGLEIVIPSSPRDVKGLLKSA
                     VASDNPTIFVDHTRLLEIRGEVADNDKPIPFGVADIKRVGSDVTLVATSYTVQVALQA
                     AEKLAKDGIEVEVVDPRTLVPFDLVTILSSVEKTHRVIIADETHQSCGVAAEIAARIA
                     ETGFDKLKAPIRRVSTLDVPVPYSPGLEAHIGPSEARIMDAVRAALA"
     gene            7913..8893
                     /locus_tag="VE170_09320"
     CDS             7913..8893
                     /locus_tag="VE170_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYJ15677.1"
                     /translation="MAIKKENSKDVIELLRRMWLIRAFEEKVSALYAERQIVGLLHLG
                     IGQEAVAVGALSLLRADDLVYGGHRSHGHAIAKGADVNKLMAEIAGRASGYCGGRGGS
                     MHIVAKECGFITATGVVGGTIPLALGAAFAAKERKKGQLTVVFFGDGAGQSGPFHESL
                     NIASLWQLPVIFVCENNGYAEFTPLSAHTKIERLAQHAKTYGIPADTVDGNDLFAVRA
                     TMRKAVDQCRAGKGPIFVECLTHRMRGHYEGDPAKYRELSQLAEWKKKDPIARVAGAL
                     KRQKALTDKELAKMASEAQALAERAAQFALASPWPQADAVADQVYAVANT"
     gene            8926..9903
                     /locus_tag="VE170_09325"
     CDS             8926..9903
                     /locus_tag="VE170_09325"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYJ15678.1"
                     /translation="MPEQTYLDAIRSGLEEEMRRDPAVYIFGEDVALGGPFGVTKGLA
                     EMFGANRTVNTPISEATVMGLAIGTATAGLRPVIEIMFIDFITLAMDQLINHAAKLHY
                     MSGGQLKIPLTVRVQCGISGAMGAHHSQSLESWLAHVPGLKVVMPANPADAKGLLKSA
                     IRDDNPVVFIEHRALYWTKGEVPANEQSVALGKAARLREGKDLTIVALSSMVAPAMTA
                     AQELAAEGISVEVIDPRTISPLDIDTIVNSVKKTGRMIVTHEAVEHGGIGAEIVSRVQ
                     QEAFYFLDSPIIRVAAPFAPVPAGPTLEKLFLPNHERIVAACRGAVAAS"
     gene            10621..10824
                     /locus_tag="VE170_09330"
     CDS             10621..10824
                     /locus_tag="VE170_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15679.1"
                     /translation="METAAATKVIAIERTEFRAVQCEHCGAKMYPQSLLQPHLDKHRL
                     HQRWLDSELRKLQETFSRMRDIA"
     gene            complement(10933..11370)
                     /locus_tag="VE170_09335"
     CDS             complement(10933..11370)
                     /locus_tag="VE170_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15680.1"
                     /translation="MDPKAQVVQLKTQLVSEGHTRFMLAETDLMTLRIHCYAPGTGEN
                     ALHAHTKEDHIFVVLDGTAQFNTERDGKTVLTATKNYAVVLPAGSYYQFCNSGQTPLV
                     LVRIGAGQDKADQRLNPDGSAIPGRAHEPNAAKPKLIEGAFFA"
     gene            11542..12411
                     /locus_tag="VE170_09340"
     CDS             11542..12411
                     /locus_tag="VE170_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ15681.1"
                     /translation="MTPLFAEIVAIFSAMGWAIDSVLVRFGLRQSNIFAAMLMSYAVS
                     ITCVWSYLIATTSLEFLTSPAMIYYLISGCMQPIFARALFYEGITRIGVARAGPLRGI
                     EPLFAATIALLVFNEQPGLQVYAGTVLIVASLWLISGKQEHDHRWRYLDALFPIGAAL
                     ISAISQTLRKQALQIIPDPFVAVAMVTTVSLFLLLGFVFTTGRAQQLRMHRSSFYFFL
                     GAALVATTAQVANFVALGRGQMSVIIPLLNTTPLFTVCFSALFLSKVETVNLRIICGA
                     LLMVAGVVLITSR"
     gene            12482..13129
                     /locus_tag="VE170_09345"
     CDS             12482..13129
                     /locus_tag="VE170_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15682.1"
                     /translation="MESREFAKLLEKQVVDFCENLDAKLPAYSFIPLTSDETRIKVMQ
                     SRLFNEIRAGEIFGGWLKSTPELDVKKTLAQATHEEYQHATFLEEALRGKGVTPHDYH
                     VLPAQMAMFNAFEGLTETVERIAAFPMAGEGVADYLIAKSLKAGSVPNWVTAPYQKIH
                     ADEEEHGNYPFEILVKYATTAEKQDKAARAVAMSLVLRRAYFDNLDRWVFEGKLY"
     gene            13241..15919
                     /gene="polA"
                     /locus_tag="VE170_09350"
     CDS             13241..15919
                     /gene="polA"
                     /locus_tag="VE170_09350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HYJ15683.1"
                     /translation="MSDKAKKPTAKNANRAMAAASTKSDAHKVLLLDGHSLAYRAFFA
                     LPDTLVTSSGQVTNAVYGFTAMLIKLLADERPQGVVVCFDKGAPQFRLDRYAEYKAGR
                     AATPDLFKQQLPLIREVLASLRIPMVELEGYEADDLLATLTKHAREEGCDVTVVTGDR
                     DILQLVRDGVAVIMNRRGISDVIRYDAPTVVERYGVTPEKWIDFVALKGEAADNLPGV
                     PGVGDKTAAQLINKYGDIEQVIVHADELTPKLREAVKNHASQVRINKELGHLLDDVRL
                     DLDPAKLRLEAWDEETVRTLFLSLELRSLHERLKALKFHGTAASPALKISSISELTSE
                     DDLRPGETLALAWNDRWLAVSDADGEARILPLSDAMPRLAGAFKDSNRQKIAHDAKGL
                     FRSALDAGVEIAGLSCDTKIAAYLLEPGSSSGYELRDTVSRYLGVSLDMEKSEPQAGE
                     QTTLSFATDDKHSVCLNAVAIRAAAPVVEEKLRAQGAWELATTLEFPLIPVLARMEHA
                     GILVDRDYLYKLNSDFGQKMTTLEQTIQQHAGEEFNVNSNLQLQRILFDKLALPKTRK
                     IKTGYSTDAAELEKLAGCHPILASLLEYREVSKLKNGFTEMLLGLIDPKSRRIHTTYE
                     QTTAATGRLSSSAPNLQNIPIRGDLGRQIRRAFVAPPGHVLLSADYSQIELRVLAHLC
                     EDETFCQAFAQGHDFHATIAAKVYAVGLVDVTSEMRNKVKQFSYGIAYGMSTYGVSQR
                     LGVEMDEAATFVETYYAQFPKVKEFLAAQVEKAKVDGFTTTMFGRRRYLPELQSSNFR
                     LRALGERMALNAPIQGSAADILKKAMIDVDAALRKHRIAKMLLTVHDELVFEVPADNI
                     GEARALIEREMTQAAELRCGLAVDMHSGANWAEAHA"
     gene            16047..>17140
                     /gene="solA"
                     /locus_tag="VE170_09355"
     CDS             16047..>17140
                     /gene="solA"
                     /locus_tag="VE170_09355"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HYJ15684.1"
                     /translation="MVDKYDTIVCGLGAMGSAAVYQLAKRANKVLGLDQYSPPHDNGS
                     SHGEIIRQAIGEGEEYVPLVLRAYELWREIEKATGWALLTITGGLTLEGQNSEVVMHG
                     RRDFLDQAVRCAEKFHIRHEILETAEIRKRFPQFAVTDERAYFEYETGFLRPKICIEA
                     QLHLARQRGAVVKTQETVVSVAGRADSAVMVTTDRGTYCAEKVIITTGPWIGRFLPPA
                     YAHLFKVYRQVMYWFDIREDCRSTFAPPGLPIFIWIFAQGSGFGFYGFPTLDGKTIKI
                     ATEQFTAISDPDHVQPAVSVDEEQSMYKDYIQERLPGVSARCRAAASCLYTTTPDSNF
                     IIDVHPDNERIMIASPCSGHGFKHSAAIGEA"
BASE COUNT         3693 a         5187 c         4841 g         3419 t
ORIGIN      
        1 cggcgaccac caaaggatag gctggtcctc cgagtaccag gggaaaatgc ccaggcggta
       61 ggccgccagc agccggtcgc tggagaggtc tccgcccact gccagcaaac cgctgggatc
      121 ggcataatcc ggcggtggaa agacgaggtc gtcaaccagt cgaaatatcg gcatgagagt
      181 ggctctgact cgctgccatt gtaacgcgaa gcggagaatt tttcacgttg gcgtgttatc
      241 cttgctttca ccgcggcgaa cagcctatgc ttcgtagcga ggagaaagcc atgttcaagc
      301 gcatcgacca tgtggccctt catgtcgccg atctcgatcg ttcggtggca ttctacgaac
      361 gacactttgg ttttaaagat tattttcgcc aaaccgctag cggcggcttg cagatagcct
      421 atttaagact ggccgatacc gtactcgaac tgacacacca cagcgatggg gcgatggccg
      481 gattccactt ttgcctggag accgacaact tcaatgagac ggtcgagcag ttacagcgcg
      541 atggcgtaaa actcgtgcgc gcaccccatg ataccgcccc ccgcgaaccg cgggaaagcg
      601 gctggcgccg ggtcgtcttc gccggccctg acggagaaca gatcgagttg cgaggctgaa
      661 gcagaatcgg gccacaggca cggtaagcaa agccaaagtt cgtgcctcac gcctaggagt
      721 tcaagatgtc caacgaaaat aaaaccatcc aagttacctg cccgtgctgc gacgcattat
      781 taaccatcga tccgattctc gccgcggtgc tcgatcataa gacgccggtg cggccgcaga
      841 tggcggtgaa tttgaaagac gccgtgaacc aggtcaaagg agaagccggc cggcgcgacg
      901 agaaattcaa gcagtttgcc gaggccgaga aaaacaaatc gaaagtttta gaagcaaaat
      961 ttcaagaact gttcaagaaa gccaaagacg aaccgctcac caaacctctc aaagatatcg
     1021 atctcgatta gcccgaatcg ttcatgcggg taaggtcgaa ttagcctctt ggctcagtga
     1081 acgcatcgca tcctcgctta aagtctgata cggcgcgcga agatatcggg cttgccctca
     1141 cggctcgata cccagaccat caagtactca ccggattgcc gcgtcggcac gatcttcgcc
     1201 gagaaatcgc tcgtgtccga gcgggttaac tggaaaacat gcgatgacgg cgaggtaatg
     1261 ctgcgcgcta ggatgtcttg acgcatgccg gtgcgcccgg aagtccaact gacaaagact
     1321 tgccctctgt cggggtcttg aaagagcgcc ggcaggatgt cgacaaactt attatcggaa
     1381 tcgccgaagg aggacaccac cggcgccgac cagaccacgg cgtcgcgcga agtggcgagc
     1441 acgacgtcgg aactgcggtc cttgtgcaac tcgagcatcg ccgacccgcg ccggtaacgg
     1501 gtccaaacta ggtaatgctc gccgctcgac gcttgcagag cgtggggaaa gtcgtcttcc
     1561 tcggcgctgc gtgtcacttg gtccggcgac agccaggtct taccgccgtc cagcgagcgg
     1621 gtcatccaaa tgtcgcggct gcccgaacgg cgcgacgacc agagaatccg caatacaccc
     1681 ttggcgtcct cgtagatcgt cggtgcccaa tcgatgccgc ggttggtgat cgactgcggc
     1741 ttgtcccacg tccggccatc gcgcgacgcg gtataccaaa catcagtgcg gccttctttt
     1801 ctttgtagtt ggcaccagac aaggtgaaat ctcccatcgc gcgtctgcat cagcgacgga
     1861 taataatcct cggcggggac atcatggatg cgccgttcct cggaccaggt gcggccatcc
     1921 gccgaactgc tgatgtacag atccacccga ccgcgctgct tggaagacca gaccacataa
     1981 aaccggccat cccgcgccaa aatcacactc gggtcttcgt tttcctcagg gctgcggctc
     2041 acccgcaccg gggcggacaa taccgggtcg gcaatcggtc ccgtatcaaa aacgatctgc
     2101 ggttgggact gcggcaaatg cagcagctgg ggcagctgtg cgcagccggc cagcaaaaat
     2161 ccgagcagta ggatggtcgt ggcaaaaatc caccgaggca atttatttgt cagttcttcg
     2221 cccaaggttt tatctcctca cagtcccgct ccgcacaggt cggctcgaca tcgtagtcaa
     2281 gatagaaata accgcgccaa tttctagctt cttcagtttg gtcccccgcc cgacactgaa
     2341 gcgcatcccc tcgttactcg tgtatcggcg aaaaacccga tcatgattgc ccaaacgagt
     2401 tttcatggca tcgccgccct ggctgtgatc gtggcccggc gcatttctag cctgcgcagc
     2461 tcgccttcta accgggactc agatgagcac gatctcctga ccgggcttaa gcgcgcgcca
     2521 ttgacgatcg ataaacaaaa cttgccacgc caactggccg caatcaaacc gatgaactgc
     2581 caccactcga tgttcctcat agttctggtt gcccgtcaaa cccttgtctc gtgcccagcg
     2641 cccagagaaa agccaaagca acgattcggc caatccacag atctttccca acaattaaac
     2701 tcgatgatga taacctgact ttcaaggcgt ggcttattcg gtggatgatt tggtttcccg
     2761 cgccggtttg gttaaaaaac aaatatgtta gcaatggtcc tcgaccatcc cggcgaagcg
     2821 ctgcgtcata gcgaaatctc gacaccggat cccggccccg gccaactctt gatccaggtc
     2881 cacgcctgcg ccgtctgccg cacggattta cacattgtcg acggcgaatt gacccaagcc
     2941 aaactgccgc tcattctcgg ccatgagatc gtcggcaagg tcgcgcaact gggcggcggc
     3001 gtcagtcaat ttaaaatcgg cgaccgcgtc ggcgttccct ggttgggctg gacctgcggt
     3061 acttgttggt attgccgcag tgaccaggaa aatctctgcg accatggcaa attcaccggc
     3121 tacaccatcg acggcggcta cgccgagtac accgtcgccg atcagcgctt ctgcttgccg
     3181 cttcccgacg gctatagcga cgcagaagcc gcgccgctgc tgtgcgccgg gctgatcggc
     3241 tatcgcagcc tggtcaaagc cggcaaggcc aaacgattgg gcctctacgg cttcggcgcc
     3301 gccgctcaca tcgttgctca agtggcgcgc tatcagcaac gcgcgatcta cgccttcacc
     3361 cgtcccggcg atgaagcagc acagcaattc gccataacct taggcgcggt atgggccggc
     3421 gggtcaaacc aaacaccgcc agagaaactc gatgccgcga ttattttcgc cccggcgggc
     3481 gaattggtgc cgctagctct caaggccgtc ggcaaaggcg gcaccgtcgt ctgcggcggc
     3541 atccatatga gcgacatccc gtctttttca tactcgatcc tctgggaaga gcgcacgcta
     3601 acatccgtcg ccaacctaac ccgccgtgac ggcgccgagt tcatggcgct ggcggcgcga
     3661 gtgcgggtgc gcacggcggt agaggcgttc gaattgcaac aagccaatga agcgttgcgc
     3721 cgattacgcg acggcaagat agaaggcgcg gcagtgctga cgacggccgc agccaatgca
     3781 caaacggtgt gacctagtct gccgtctgca acggcgcaat cacttcccgc gctaagattt
     3841 ccacctgttc gcgccaaccg ttgaacggtc cgcaaggacg catgacgaat ttgctcgcgc
     3901 cggcggcgat gtaggcttgg actctggcgc ggacgtggtc gggggtgccg aaggcggtga
     3961 attcactggt cggcagatca ctccgcgggc ggatcgaacg gccgccaagc tcggcagctt
     4021 ttacacgctc ggcagcgaag taaaaaggta tcagcacgcc gtaatggtct tcttcgaccc
     4081 gtcggccggc atcggcggca tacctgcgaa tcgattcgat cccggttgca acttcagccg
     4141 gcgacaccga tgagaccagc cagccgtcgc ccaatcttcc ggttctcttg agggcggctt
     4201 cactccgccc gccgatccag atcggcagcc catttttttg ccatggccgc ggtaggatcg
     4261 tcacgtcttc aaccgtaaag tacttgccgg cgaagtttac attgtcttcg gtccacaagc
     4321 gccgcatgag cacgatcatt tcatcggcgc gcccggcgcg ttcttttttg tttatgccga
     4381 cggcctgcaa gtccttcgac tcatcgccgc cgagaccgat ggccggaaac aaccggcctt
     4441 gggagagaaa gtccagggtc gcgagttctt tggcgagtac caccggatgg cgcgtcggca
     4501 gcaccagcgt gctggtacca aacttcatct tgcgcagccg tccggcgacg taagcaagaa
     4561 aagtaacggg ttcgagggtt aatgcggtag aagccagccg gtcgctgagc cagatggagt
     4621 caacatcgag cgattcgaaa tagtcgacca gatcgagcac cgcctcgggc cgcatgtccc
     4681 ggctcggcca ttggccgagc tgtatcccga tgcggacttt catctttgcc aattactttt
     4741 cgtaaagctt cttgatgaaa ccgctcttgt cgagttcttc aacgaagctg gcgtcgacga
     4801 aatcctccgg cttatatttg ttgatctccg ggcgcgtcgc ggcgaggtat tcgatgctgg
     4861 ttttcatgcc tttcaagttg gggtagggcg ccgacaagcg gtgtttggcg aagagttccc
     4921 aggattcctg cgccatcggg tcgtcgttta accgcaacat ttttttgatc acccgcttgc
     4981 cgagctcagg ctcttgcttg aagcgggcaa tgccttcgat atgggcgcgc atatagcgca
     5041 tcaccaaatc gcggtcctgc ttgatctgag tgcgggtcgt ggcgatctcg ctccagggat
     5101 agtcgacctc tttgaaaaaa tcccacaaca catgaaagcc ggcgcgttgc gcatagatat
     5161 agttcggatg ggacagcgtt gacatgggaa tccgcccctg ctgcatggcg atgaaccggt
     5221 cggcatcgct gccgatctgc agcatggcaa agtctttgtc gggattgagc cctttttgcc
     5281 gcgccagata ttttaccagg aagtcgttga gcgaaccgaa ggtggtcacg ccgatggtct
     5341 tgcccttgat gtcttcggtg cgtttgatgt ccggtcgcgc catcaagtat tgaatgacac
     5401 ccggcatggt gtgtgccaag attaccatgt cggaaccgga aagaaccgaa ttcatcactg
     5461 tcggcgtggc gatgggcgag atttgcacgc ttccggagat cagcgccttc gaggcgatcc
     5521 ccgaccccgc caccgcgatg ggctcgacat cgaggccatg cttctcgaaa atcttcgctt
     5581 ctttggtgaa ataaataatg atgttgccca tgctgatggc gggcacgccg acctgaactt
     5641 ttttaagccc gcgagtctgt gccatgagcg tggtaggaag caccaataaa gccagcaata
     5701 gcgccggcca cgtgcgccgt gaattcatta cgcctccgtc ggaatgccga tcttttatgg
     5761 gcatttagtc ccgccattaa cggcttgtca atccattctc ccggcgttga caacggccgg
     5821 ccgtttgaat taagtcaggt gcatcatgtt accgaactta ccgagagaaa atttgttgga
     5881 gctgtgccgg cggatgttcg tcatccgcca cttcgaagag aatctcgtgg ggctgcacga
     5941 caagggcgtg ttcggcggcc actaccattt gtatgtcggc caggaagcga ccggcgtgcc
     6001 ggcgcttgcc tgcctggatg ccgacgacta tctcttcacc acgcacagaa atcatggcca
     6061 tctgcttgcg cgcggcgctg agccgaaaaa actcttcgcg gaaatcttgg gccgcgtcga
     6121 cggcttgaat aaaggcaagg gcggttcgtt tcattcggtg gcgccagggc tgggattcct
     6181 ccatagctcg ggcgtcgtcg ccggcatcat cccgctctcc accggcaccg cctacgcttc
     6241 caaagcgcag aaaaatggcc gtctcaccgt ttgcctgttc ggtgacggct cccttgaaga
     6301 aggcgccgca ccggaagcgt tcaatatcgc ctcgctgctg aaactgccgg tgctgtttct
     6361 ctgcgagaac aacggtaagt atggcgaggg ccgtgccgtt gccgcggcgc agtctggttc
     6421 catggccgcc tatccgctca ccgatttacc gaaagcttat aaaatcccgg cgcaacagat
     6481 cgatggcata gacgccggct tggtgcatgc aacgatctcg gaagcggtcg agaaaattcg
     6541 ccgcggcgaa ggtccgcaat tcgtcgaagc gcagacggtt cgctatcccg gcagcgcgac
     6601 caattggccc accgtgccgg cgccgacgga cacaacattg gcgtgggacg taagccaggt
     6661 gccggagaat gctcgtgagt ggtaccgcag cgcggatcca ttattggtct atgtccgcga
     6721 gttggtcgaa gcgaatcaag ccaagcgcga agaaatcgcc gccctcgaaa gccaagtgaa
     6781 atccgccatc gccgccgccg tcgacttcgc cgtcaacagt ccttacccga aaccggaaga
     6841 ggcggagaga gactttttcg ccaagagtcc attatgagag aacttgctta ctacgaagct
     6901 aaattcgagg ccctcgacga gatcatgagc ggcgacgagc gcgtccatct gatcaacggg
     6961 actttcatga gcttgagccc gcatcgtcac gtctatcaaa acctgcaaaa aaaattcaaa
     7021 gcgcggattt tttccccgcc gatctccgaa ctcggcttct gcggtctcgc caccggcgcg
     7081 gcgatggcgg gacttcgccc gatcgtcgat ttgagcaccg ggagttttat ctatgaagcc
     7141 tggccccagg tcgccaacga agcggccaat gcttactcca tgtccggcgg ccagacccgc
     7201 gtgccggtgg tgtttcatct attccacgga ctgcgcggcg gcggcgcggc gcagcattcg
     7261 ggcagcccgc aggcgatgct gtggaacgtc cccggactgg aaatcgtcat cccgtcctcg
     7321 ccgcgcgacg tcaaaggcct gttgaagtcc gccgtcgcca gcgacaaccc aaccatcttc
     7381 gtcgatcaca cccggctctt ggagatccgc ggcgaggtgg ctgataacga caaaccgatt
     7441 cccttcggcg tcgccgacat caagcgcgtt ggcagcgatg tcacgttagt cgcgacttct
     7501 tacacagtcc aagtcgcgct ccaagccgcg gaaaaactcg ccaaggacgg cattgaggtc
     7561 gaagtcgtcg atccgcgtac cctcgtgccc ttcgacttgg tgacgattct atcttcggtg
     7621 gagaaaaccc atcgggtgat catcgccgac gagacccatc agagctgcgg cgtcgccgcc
     7681 gagatcgccg cacgcatcgc cgagaccggt ttcgacaagc tcaaagcccc catccgccgg
     7741 gtctcgaccc tcgacgtgcc ggtgccctac agcccagggc tggaagcgca catcggccca
     7801 tccgaggcgc gcatcatgga cgcggtacgc gcggccctgg cttaaaaaca gtttcgggtt
     7861 ctgcaatctg cgatgacctg ccgaacagca cttctacggc tcgctttgtt tcatggctat
     7921 taagaaagaa aactccaagg acgtaatcga gttgttgcgc cgcatgtggc tgatcagagc
     7981 gttcgaggaa aaagtctcgg cactctacgc cgaacggcag atcgtcggcc tgctgcattt
     8041 gggtattggc caagaagccg tcgccgtcgg cgcgctcagt ttgctacgcg ctgacgatct
     8101 cgtgtatggc ggccaccgct cccacggtca cgctatcgcc aagggcgcgg acgtaaataa
     8161 attaatggcc gagattgccg gccgcgccag cggttactgc ggcggcaggg gcggctcgat
     8221 gcatatcgtc gccaaagagt gcggcttcat caccgccacg ggcgtggtcg gcggcaccat
     8281 tccgctcgcc ctcggcgcag ccttcgccgc gaaagaaaga aaaaagggcc agctcacggt
     8341 cgtcttcttc ggcgacggcg ccggccaatc cgggccattt catgagtccc tcaacatcgc
     8401 cagcctctgg caactgccgg tgatctttgt ctgtgagaac aacggctatg ccgagttcac
     8461 cccgctatcg gcccatacca agatcgaacg cctcgcgcag catgccaaga cctatgggat
     8521 tcccgccgac actgtcgacg gcaacgatct atttgccgtg cgcgcgacga tgcggaaagc
     8581 cgtagaccaa tgccgcgccg gcaaaggtcc gatattcgtc gaatgtttga cccatcgcat
     8641 gcgcggccac tatgagggcg atcccgccaa gtaccgcgag ctatcccagc tcgccgaatg
     8701 gaagaagaaa gatcccatcg ctcgggtcgc gggcgcgtta aaaaggcaga aggcgctcac
     8761 cgataaagaa ctggcgaaga tggcaagcga agctcaagcg ctggcagaaa gggcggcgca
     8821 atttgctcta gcgtcgccat ggccccaagc cgacgcagtc gccgatcagg tctacgccgt
     8881 ggcaaacact taggcgaaat cttttctcct ctgaagatat tcaccatgcc cgaacaaacc
     8941 tatctcgatg cgatccgcag cggactggaa gaagaaatgc gccgcgaccc ggcggtttac
     9001 atctttggcg aagacgtcgc cctcggcggc cccttcggcg tcaccaaagg cttggctgaa
     9061 atgttcggcg ccaatcgcac cgtcaatacg ccgatcagcg aagcgacggt catgggactc
     9121 gccatcggca ccgccaccgc cggcctcaga ccggtaatcg aaatcatgtt tatcgatttt
     9181 atcaccctgg cgatggatca actgatcaat cacgccgcca agcttcatta tatgtcgggc
     9241 gggcaactga agattccgct gaccgtacgc gtccagtgcg gcatcagcgg cgccatggga
     9301 gcgcaccatt cgcaaagtct ggagtcatgg ctggcccatg tgcccggcct gaaagtggtc
     9361 atgccggcca atcccgccga cgccaagggt ctgcttaaaa gcgccatccg cgacgacaat
     9421 ccggtggtgt tcatcgaaca tcgcgctctc tattggacca agggcgaggt gccggcgaac
     9481 gaacagagcg tcgcccttgg taaagccgcg cggttacgcg aagggaagga tctcaccatc
     9541 gtcgcgcttt ccagcatggt ggcgccggcg atgacggcgg cgcaggaatt agccgcagaa
     9601 ggcatctcgg tggaagtcat cgacccacgg acgatttcac ccctcgatat cgacaccatc
     9661 gtcaactcgg tgaaaaaaac cggccgcatg atcgtcaccc atgaagcggt cgaacatggc
     9721 ggcatcggcg cggaaatcgt cagccgggtg cagcaggaag ctttttattt tctagatagc
     9781 ccgatcatac gggtcgccgc cccgttcgca ccggtgccgg cgggaccgac gctggaaaaa
     9841 ctgtttttgc cgaaccacga gcgcatcgtc gccgcgtgcc gcggcgcggt cgcagcaagc
     9901 taatctacga gcaaccgatg ccgcggccaa ccggttcgat tgacaaatcg tagctaccgg
     9961 ccgctcacgc gctgcggctt gatacaggcg gcgcaaaggg cgcgcgtgcc ggatagccgc
    10021 ttcgataggc gcccatcggg cagagcgacc acgtgtaact cctggccaat gtatttagca
    10081 tccaggtcgc cgtaaccggc gccgcggttc gccggcgtac aaccgatctc ttatcaagaa
    10141 ccctaaaagc gcatcccgat gaattcatcg gcatcgccgc cctatcgcga cgacaattca
    10201 cgccgttgtc gaaggcgatt tcgcttcagg gagtggattg catcaaatca tctttcacaa
    10261 cggcagagat tttgacagcg aatttctctg gcggttctat gcagcgcccg ctggatcgct
    10321 tggatcctag accgcttaga aatggtttat gaatagcccc gtcccgcggc acacgaagga
    10381 cctgcccgcg gaacgactcc cggcagattc ggcgtaccgt agctgcagtc agtcaacttg
    10441 acctacgcta tgtcaccatc gacgtggtct gacaactcgc ccctgtcacg attgccctgc
    10501 agtgaatttt ctcacgacaa gtttcaataa agtcggtgcc ttgcgaccct ggccacaact
    10561 gaagcaagtg gcatacggct tgcgttactg acgtctatgc agtttgacag gaggctggac
    10621 atggaaacgg cggcagcgac aaaagtcatc gccatcgaac gcacagaatt tcgcgcggtt
    10681 cagtgcgaac attgcggtgc caagatgtat cctcaatctt tgctgcaacc acacctggac
    10741 aaacaccgcc tgcatcaacg ctggctcgac agtgaactgc gcaagctgca agaaactttt
    10801 tcgcgcatgc gcgacatcgc ttaatttagg ttctgcgaac ttccccataa ggggaagctc
    10861 ctccccgacc aacccgcgtc agcccccagg cgcgggttgg tccccctccc aaaagaaaat
    10921 aatcgccggg atttacgcaa agaacgcccc ttcgatcaat ttgggttttg ccgcgttcgg
    10981 ctcgtgggcc cggccgggaa tcgccgagcc atcgggattg agccgctggt cagccttgtc
    11041 ctgacccgcg ccgatgcgca ccagcaccag cggtgtctgg ccggaattac agaactgata
    11101 gtaactgcct gccggcaaca ccaccgcgta attcttggtc gccgtcaaca cggttttgcc
    11161 gtcgcgttcg gtgttgaatt gcgccgtgcc gtccaaaacc acaaaaatat gatcttcctt
    11221 agtatgcgcg tgcagcgcat tctcgccggt gcccggcgcg tagcagtgaa tgcgcagggt
    11281 catcaaatcg gtttcggcga gcatgaagcg ggtatgcccc tcgctcacta gttgagtttt
    11341 tagttgcacg acttgcgctt ttggatccat gaattctcct ttgccgcata gtgttaaaga
    11401 ccgccgatct taggatagac gcggcgccaa ctgcaagcgc tttggccaaa ccgtcgatgc
    11461 ttcgcggcca agatcggacc accggttgca aatcgcgctg ccctcggctt agctgaaggt
    11521 cgccaaagat cgggacatat cttgacacca ttgttcgctg aaatcgtcgc gattttctcc
    11581 gccatgggct gggccatcga ttcagtgctg gtgcgcttcg gcctgcgcca atccaacatc
    11641 ttcgccgcca tgctgatgag ttacgcggtc tccatcacct gcgtctggag ttacctgatc
    11701 gccacgacgt cgttggagtt tctcacttcg ccggcgatga tttactactt gatcagtggc
    11761 tgcatgcagc cgattttcgc ccgcgcgctt ttctacgagg gcatcacccg cataggcgtc
    11821 gcccgcgccg gaccgctgcg cggtatcgag ccgctcttcg ccgcgaccat cgcgctgctc
    11881 gtgttcaacg agcagccggg gctgcaggtc tatgccggca cggttttgat cgtcgccagc
    11941 ctttggctga tctccggcaa gcaggaacat gaccaccgtt ggcgctatct cgacgcgctc
    12001 tttccgatcg gcgcggcatt gatctccgcg atctcgcaga ccctgcgcaa acaagcgctg
    12061 cagatcatcc ccgatccgtt tgtcgccgtg gcgatggtca ccaccgtttc gctgtttctg
    12121 ttgctgggct tcgttttcac caccggccgc gcccagcagt tgcgcatgca tcgcagcagc
    12181 ttttacttct ttctcggcgc ggcgctggtt gccaccacag cgcaggtcgc caacttcgtc
    12241 gcgttgggcc gcggccaaat gtcggtgatc attcccttgc tcaacaccac gccgctattc
    12301 acggtatgtt ttagcgccct gttcctgagc aaggtggaaa ccgtcaacct gcgcatcatc
    12361 tgcggcgcgc tcctcatggt cgccggcgtc gtgctgatta ccagccgctg aggattgacc
    12421 cggtgaattc aaattgatag ccaggactcg aacgagaaac gggggcaacg caggagcgct
    12481 tatggaaagc cgggaattcg ccaaattgct ggaaaaacaa gtggtcgatt tctgcgaaaa
    12541 cctagatgcg aagctgccgg cctacagctt catcccgctc accagtgatg agacgcgcat
    12601 taaggtaatg cagagccgcc tgttcaacga aatccgcgcc ggtgagatct tcggcggctg
    12661 gttgaaaagc acgccggagc tggacgtgaa gaaaaccctg gcacaggcga ctcatgaaga
    12721 ataccaacac gcgacattct tggaagaagc cttgcgcggc aaaggcgtca cgccgcacga
    12781 ttatcacgtg ctgcccgcac agatggcgat gttcaatgct tttgagggac taaccgagac
    12841 cgtggaacgc attgccgcgt tcccgatggc cggcgaaggc gtcgccgatt atctcatcgc
    12901 caaaagtctg aaggccggct cggtgcccaa ctgggtcacc gccccctacc aaaaaatcca
    12961 cgcggacgaa gaagaacatg gcaactaccc cttcgagatc ctggtcaaat acgccaccac
    13021 cgcggagaaa caagataaag ccgcccgcgc tgtggcgatg agcttagtcc tgcgccgcgc
    13081 ctatttcgat aatctcgatc ggtgggtgtt tgaaggaaag ctgtactagc gaatatcgcc
    13141 gccgcgcact cagcgaccaa cataaataga tcgcgccggg ttctgcccga tgacggcgcg
    13201 cgatattgcc gcaagcgaat tccccggtta aggttactgc atgagcgaca aagccaaaaa
    13261 accaactgca aagaacgcta accgtgcgat ggctgccgca tcgaccaagt cggacgctca
    13321 caaagtgctc ttgctcgacg gccattcact ggcctaccgc gcctttttcg cactgcccga
    13381 tactctcgtc acgtcgtccg ggcaagtgac caatgcggtc tacggcttca cggcgatgct
    13441 gatcaagctt ctcgccgacg agcggccgca gggggtagtg gtttgcttcg acaagggtgc
    13501 gccgcaattt cgcctcgacc gctacgcaga gtacaaggcc gggcgggcgg caaccccgga
    13561 tttgttcaag cagcagctgc cgctgattcg cgaagtgctc gccagcctgc ggattcccat
    13621 ggtcgagctg gaaggctatg aggccgacga cctgctggcg accctgacca agcacgcccg
    13681 agaagaaggt tgcgacgtga ctgtcgtcac cggcgaccgc gatattcttc aactggttcg
    13741 cgacggcgtc gccgtcatca tgaaccggcg cggcatttcc gacgtgattc gctacgatgc
    13801 gccgactgtc gtcgagcgct acggcgtgac accggagaag tggatcgatt tcgtcgcgct
    13861 taagggtgaa gccgcggaca acttgccggg agtgcccgga gttggcgaca agaccgcggc
    13921 tcagctcatc aataaatacg gcgatatcga gcaggtcatc gtccatgccg acgagctgac
    13981 tccgaaactt cgtgaagcgg tcaagaacca cgcgagtcag gtgcggatca ataaagagct
    14041 gggccatctg ctcgacgacg tgcgactgga cctcgatccg gcgaaactcc gcctcgaagc
    14101 ctgggacgag gaaacggtgc gcaccctctt tctctcgctc gagcttcgca gcctgcacga
    14161 gcgactcaag gcgctcaagt ttcacggcac ggcggcgtcg cccgcgctca agattagttc
    14221 gatttcggag ctgacttcgg aggacgatct acgtcccggg gaaaccctcg cgcttgcttg
    14281 gaatgatcgg tggcttgccg tgagtgatgc agatggagaa gcgcgtattc tgccattgag
    14341 tgatgccatg cccaggctgg ccggcgcgtt caaagattct aaccggcaaa aaatcgccca
    14401 tgacgccaaa gggcttttcc gctccgccct cgacgccgga gtcgaaatcg caggcttgag
    14461 ttgcgatacc aagatcgctg cctatctcct cgaacccggt tcctcttcgg ggtacgagct
    14521 gcgtgatacc gtgagccgtt atctcggcgt cagcctggat atggagaaga gcgagcccca
    14581 agcgggcgag caaaccaccc tgtcttttgc aaccgacgac aagcacagtg tttgtctgaa
    14641 tgccgtggcg atccgagcag cggcgccggt ggtcgaggag aaactccgcg cccaaggcgc
    14701 ctgggaactt gccaccaccc tggagtttcc tctgatcccg gtgctcgcgc gcatggagca
    14761 cgccggaatt ctcgtcgatc gcgactatct ctataagctg aattccgact tcggccaaaa
    14821 aatgaccacc ctggagcaga ccattcaaca gcacgccggc gaagaattca acgtcaattc
    14881 caatttgcag ctgcagcgca ttcttttcga caaactggca ttgccgaaaa ctcgaaaaat
    14941 caaaaccggc tactccacgg acgcggctga attggagaag cttgccggtt gccatccgat
    15001 tctcgcctcc ctgctcgaat accgcgaggt atcgaagctc aaaaatggtt tcaccgagat
    15061 gctcctcggc ctcatcgatc cgaagagccg gcgcatccat acgacctacg aacaaaccac
    15121 ggcggccacc gggcgcttgt catcgagcgc gcccaacttg caaaatatac ccatccgcgg
    15181 cgatctcgga cggcagatcc gccgcgcttt cgtcgcgccg ccggggcatg tgttgctgtc
    15241 ggcggattat tcgcagatcg agctgcgcgt gctcgcgcat ctttgcgaag acgagacgtt
    15301 ttgccaggca ttcgcgcagg ggcacgactt tcacgcgacc attgcggcga aagtctatgc
    15361 cgtcggattg gtcgacgtca cttccgagat gcgcaataag gtcaagcaat tttcctacgg
    15421 catcgcttac ggtatgtcga cctacggcgt ctcgcaacgg ctcggcgtcg aaatggacga
    15481 agcggcaact ttcgtcgaaa cctactacgc gcaatttccg aaagtgaaag aatttctcgc
    15541 cgcgcaggtg gaaaaagcaa aggtcgacgg ctttaccacg accatgttcg gccggcgccg
    15601 ttatctgccg gagttgcagt cttcgaattt tcggttgcgc gcattgggcg agcgcatggc
    15661 gctcaacgcg ccgatccagg gaagcgccgc cgacattctc aagaaagcga tgatcgacgt
    15721 cgacgccgca ttgcgcaaac accggatcgc caaaatgctg ctgacggtcc acgacgagct
    15781 ggttttcgaa gtgcccgcgg acaacatcgg tgaagcgcgc gcattgatcg aaagagaaat
    15841 gacacaagcc gccgagctgc gctgcggttt ggccgtggac atgcacagcg gcgccaattg
    15901 ggccgaagcg cacgcgtgaa cctacgcgtg ctcgttcaaa agcttcaagc cgtgcaaccg
    15961 cttcgctccg ttcaagccgt gtaaccaatc acggaacacg catacttgcg gaacccgatc
    16021 ggtgtcgcgg gccgaaaaac aaagcaatgg tcgacaagta cgacacgatc gtatgcggtc
    16081 tcggcgccat gggaagcgcc gcggtttacc aactggccaa acgggccaac aaagtgcttg
    16141 gcctcgatca gtattccccg ccccacgata acggctcatc tcatggcgag attattcgcc
    16201 aagcgatcgg agagggcgag gagtatgtgc cgttggtgtt gcgcgcctat gaactgtggc
    16261 gcgagatcga aaaagccacc ggctgggcac tcctgacgat cacgggcggc ctaaccctgg
    16321 agggtcaaaa cagcgaggtc gtcatgcacg gccggcgtga ttttttagac caagcggtcc
    16381 gctgtgccga aaagttccat atccgtcatg aaatcctcga aaccgcggag ataagaaaac
    16441 gctttccgca gttcgccgta accgatgagc gcgcttactt tgaatatgaa acgggatttc
    16501 tacggccaaa aatttgtatc gaggcccaac tgcatctcgc gcgccagcgc ggcgcagttg
    16561 tcaaaactca agagaccgtt gtgtcagtcg ccggccgcgc ggactcggca gtaatggtca
    16621 cgaccgaccg cggcacttat tgcgccgaga aggtcatcat caccaccgga ccctggatcg
    16681 gccgtttttt accgccggca tacgcgcatc tgttcaaggt gtaccgccaa gtcatgtact
    16741 ggttcgacat cagggaagat tgccgctcga catttgcgcc acccggcctg ccgattttca
    16801 tatggatatt tgcccagggc agcgggtttg gtttctatgg gtttccaacc ctcgacggca
    16861 aaacgatcaa gatcgccacc gagcaattta ctgcgatcag cgatccagat cacgtgcagc
    16921 cggcggtcag cgtggacgaa gagcagtcca tgtacaaaga ttatatacaa gagcgtttac
    16981 cgggagtttc cgctcgttgc agagctgcgg ctagttgcct gtatacgaca acgcccgatt
    17041 ccaacttcat catagacgtt cacccggata acgaacgcat catgattgcg tcgccctgtt
    17101 caggccatgg attcaaacat tcggcggcga tcggagaagc
//
LOCUS       DASQKS010000359         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1861603, whole genome shotgun sequence.
ACCESSION   DASQKS010000359 DASQKS010000000
VERSION     DASQKS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861603"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VE170_09360"
     CDS             <1..275
                     /locus_tag="VE170_09360"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HYJ15685.1"
                     /translation="GEIIYVDSSGNLFTNIHGRDLTELPRKRLLVGLAGSPLGGLVTN
                     YASAGAGEFACLLNSWGLLEIAVNQDSAKKRTSAKIGDKVTVTVAD"
     gene            292..750
                     /locus_tag="VE170_09365"
     CDS             292..750
                     /locus_tag="VE170_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HYJ15686.1"
                     /translation="MSERLSWDQYFMTITLQVAERSTCTRAKVGAVVARDKNILATGY
                     NGAPAGMPHCTDVGCLIYESKTPNGDTEQNCFRTIHAEMNAIAQAAKNGSSIKEAAIY
                     ITHTPCIHCLKVLVNTGIKEIYYLNPYKLHTLDELMRYTQVKFHKVDLPG"
     gene            complement(1002..1319)
                     /locus_tag="VE170_09370"
     CDS             complement(1002..1319)
                     /locus_tag="VE170_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HYJ15687.1"
                     /translation="MKKLFAFVATFMMAVMASGVAMAADSAAGDSKSVAIAISAGVAV
                     AIAAFGAALGQGRVGAAAMESIGRNPNAADKLFLPLVLTLALLEALALYGFVIAILLQ
                     GKI"
     gene            complement(1389..2126)
                     /gene="atpB"
                     /locus_tag="VE170_09375"
     CDS             complement(1389..2126)
                     /gene="atpB"
                     /locus_tag="VE170_09375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HYJ15688.1"
                     /translation="MFGDHTFFAIIAGLLLIWFAVKARGALVKASDPVVPAAELGSRN
                     IAELIVQLIISQSDAIIGKAGRKYVPFYGTFFFFILLSNLMGLLPGFAPPTGNLNTTI
                     GLALVSFIGYNVIGVREQGVAYFKHFVGPMTSLPGSSIVSKLSLLPVLLISVAFFFIL
                     ELFSHGFRPVSLSLRLFGNMMGDHEVIGAFTSLTKLVVPVAFYLMGTLVCLIQAFVFT
                     LLSMIYVALAVSHGHDEEHAHDAQQHH"
     gene            complement(2184..2483)
                     /locus_tag="VE170_09380"
     CDS             complement(2184..2483)
                     /locus_tag="VE170_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15689.1"
                     /translation="MGRGTAIDGMALIAGGVFMGVNFFLLSFGVAAVLTPLASKGKIK
                     LGVGLLVLKVLLFLALLMMVFFRFKFDAISFALGFSTLIVAIVVEAVRKSLAVEN"
     gene            complement(2846..3631)
                     /locus_tag="VE170_09385"
     CDS             complement(2846..3631)
                     /locus_tag="VE170_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ15690.1"
                     /translation="MPASIARETLTARYNDLKSVQKILHRYRNDVAAVIVEPVCGNMG
                     VILPQADFLRDLRNLTTQSDVLLIFDEVITGFRIALGGAQHRYGIKPDLTCLGKILGG
                     GLPLAAFGGRRRIMDQLAPLGPVYQAGTLSGNPLAVAAGMETLRMLGRRGSYETLEAK
                     GQRLANGLNDVIRKHEIRATINRIGSMLTLFFGVDHVATVDDARRCDRKQFASFFHGM
                     LERGIYLPPAPFEAWFVSLAHSNADLDRTISAFDDWAGSADRG"
BASE COUNT          997 a         1000 c          918 g          763 t
ORIGIN      
        1 aaggtgaaat catctacgtc gacagctctg gcaacctgtt tacaaacatc cacgggcgtg
       61 atttgacaga gctaccgaga aagcgattgc ttgtaggctt agccggtagt cccctaggcg
      121 gtttagttac caactacgcg agcgccggcg ccggcgagtt cgcctgtcta ctcaatagct
      181 ggggcttgct ggaaatcgcg gttaatcagg atagcgccaa aaagcgaacc agcgcgaaaa
      241 tcggcgataa agtgactgtt acggtcgcgg actagaaggc cagaggcatt tatgagcgaa
      301 cggctgagct gggatcaata tttcatgacg attaccttgc aggtggccga gcggtcgacc
      361 tgcacgcgtg ccaaagtcgg cgccgtggtc gcccgtgaca agaatattct cgcaactgga
      421 tacaacggcg cgcccgccgg catgccccat tgcaccgacg taggatgttt gatttacgaa
      481 tccaaaacac ccaacgggga caccgaacag aactgttttc gcaccatcca cgctgaaatg
      541 aacgcaatcg cccaggccgc caagaacggc tcgagtataa aggaggcggc gatctacatt
      601 acccacactc cctgcattca ttgtcttaag gtcttggtga acaccgggat aaaggaaatc
      661 tattatctca atccctacaa acttcatacc ctcgatgaac tcatgcgtta cacgcaggtg
      721 aagttccata aagttgattt gccgggatag gaattaagca gcagccggct cgattgccgg
      781 gggattttga actaggtcgg ctggaagtcg actgctgtaa ccgtttttcc cctcgaaagc
      841 ggaatccagg ttcttcgcag tcaacacagt cccgtcatct tgaatgcacg agcggctttg
      901 acgctactcg tcgcggaagg cgattgatat tgcagcccac aaaaaaaggg tgaagagaag
      961 tctcttcacc ctttaatggt cgaatgcgtc gtttttcgca gttagatctt gccttgcaag
     1021 agaattgcga tgacgaaacc gtaaagcgct aaagcttcaa gcagcgctag tgtcagcact
     1081 agcggcaaga agagcttatc agcggcattg ggattgcgcc cgatgctttc cattgcggcc
     1141 gctccgacac gaccctggcc caaggcggcg ccgaaggcag cgatcgcgac ggcgacaccg
     1201 gcggaaatcg caatagcgac cgactttgaa tcgcccgctg cgctatccgc ggccatcgct
     1261 acaccactgg ccattacggc catcatgaag gttgcaacaa aggcaaacag ctttttcatc
     1321 ttttgctcct ttctagcgat ttttggcctg ccactcctct gtcctcttcc tcaccgcgcg
     1381 gccgtcgctt aatgatgttg ctgggcatcg tgagcatgct cttcgtcatg tccgtgactg
     1441 acggccaggg cgacgtaaat catactcagc agcgtaaaaa caaaagcctg aatcagacaa
     1501 accaaggttc ccatcagata gaatgccacc ggaacgacta gtttagttaa gctggtaaac
     1561 gcgccgatca cttcatgatc gcccatcata ttgccgaaaa gacgcaaaga cagggagacc
     1621 ggacgaaaac catgggaaaa cagttcaaga ataaagaaaa acgccacgga gatcagcagt
     1681 accggcagca aagacagttt tgagacaatg ctacttccgg ggagactggt cataggaccg
     1741 acaaaatgtt tgaagtaggc gacgccttgc tcgcgcacac cgatgacgtt gtagccgata
     1801 aatgatacca gggcgagacc gatggtggta tttaaattcc ctgtcggtgg agcgaagccc
     1861 ggcaaaagac ccatcaagtt actcaagaga ataaagaaaa agaaagtacc gtaaaacggc
     1921 acatacttgc gcccagcttt gccaataatc gcatcactct gactgatgat aagttggacg
     1981 atgagctcgg caatatttct cgagccaagt tccgccgccg gcacaaccgg gtcgctagcc
     2041 ttaaccaacg cgccacgagc tttgacggca aaccatatta gcaacagacc ggcgataatg
     2101 gcgaagaacg tatgatcgcc gaacatatgc tgcacactct ccggcagcgc gttataccaa
     2161 gtatacggat gttccatatc tccctagttc tccaccgcta gcgattttct cactgcctcg
     2221 acaacgatgg caacgatcaa agtcgaaaaa cccagcgcaa aggaaattgc gtcaaattta
     2281 aagcgaaaaa agaccatcat taataaagct agaaataata ggacctttaa caccaacaag
     2341 cctactccga gtttgatttt acccttacta gcgagcggcg tcagaaccgc cgcgacgccg
     2401 aagctaagca ggaaaaaatt gactcccatg aatacgccgc cggcaatcag cgccatgcca
     2461 tcgatggcgg taccccgccc caaggctaaa aacagtgtcg ctaataagac cgcatgccag
     2521 cattcaatcc tcaggatggt gttccgggcc gttgcttcca ttccgattgc gcgcaaaaaa
     2581 tttcacccaa cgtatcaacc ggacgaacgc tccgacaaaa gctaacgccg tgagcgatat
     2641 gagaaaccac ggcgaggtat gaagatattc gtcgagaaaa tagccgacca ccaagccacc
     2701 tataatggtg cctggaagtt cgaaagcaac tcccaaatat aatcctgccc tgcgcaggag
     2761 cggagcttga ctatcgtgcc ggtgattgag gcgctcctca attttcccca ctgggctagt
     2821 tttttaacat gaagattatg ctggatcaac cgcgatctgc gctcccggcc caatcgtcaa
     2881 aagcagaaat tgtcctgtcc aaatcagcat ttgagtgggc tagggagaca aaccaagcct
     2941 caaaaggcgc gggcggcaaa taaatcccgc gttcaagcat gccatgaaaa aaacttgcga
     3001 actgtttgcg atcacagcgg cgcgcatcat ccacggtggc gacgtgatcg acaccaaaga
     3061 acaaagtcaa catcgatccg atgcggttga tggttgccct aatttcgtgt ttgcggatga
     3121 catcatttaa accattcgca agtctttgcc ctttggcttc gagagtttcg tagctgccac
     3181 gccgcccaag catgcgcaac gtttccatac cggcggcaac cgccagcgga ttgcccgaca
     3241 aagttccggc ctggtagaca ggcccgagcg gcgctagctg gtccatgatt cgtcgacgcc
     3301 cgccgaaagc agccaacggt aagccgccgc caagtatttt ccccaaacat gttaagtcag
     3361 gtttgattcc atagcgatgc tgggcgccgc ctagagcgat acgaaatccg gtgatcacct
     3421 catcgaagat cagtaaaaca tctgactgtg ttgtcaggtt gcgcaggtcg cggagaaaat
     3481 cggcttgagg aagtatcact cccatattgc cgcaaaccgg ttcgacaatc actgcagcga
     3541 cgtcgttacg ataacgatgc aatatttttt gcacagactt taaatcgttg tacctagctg
     3601 tcagtgtttc acgtgcgatg ctcgccggca cgccgggact atcgggcaga ccgagagtgg
     3661 caacaccgga accggctt
//
LOCUS       DASQKS010000360         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2302599, whole genome shotgun sequence.
ACCESSION   DASQKS010000360 DASQKS010000000
VERSION     DASQKS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302599"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VE170_09390"
     CDS             <1..636
                     /locus_tag="VE170_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15691.1"
                     /translation="RIGLTSIGGTTWMALLLWLEHFGLDVQRDKMQLQALGEQAITVQ
                     SLEAGIVNAAIFEGIFSRRLKAKGFNTLGEYSALKHQFLSQALVVQRSFLQQRSDTLE
                     NLLKAEIEAVAHVLAPKNKPAMIKTLMRRMKTDATAAEEGYLDLLRAMERKPVPTVES
                     MIHVQRLMKIQNPKIGDVKLDDINDARLIKKLDDSGFIDNAYAAQGISAKQ"
     gene            720..1751
                     /locus_tag="VE170_09395"
     CDS             720..1751
                     /locus_tag="VE170_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15692.1"
                     /translation="MKHLRRLTPLFLLALGLTALLWSLPAFGASAPGKIILGYAAPGA
                     RALPFWVAQDLGLFNKYGVDVEPVFIRGAPILVAGLASGDIHVGSTGGSATLAAVAGG
                     QDLKIIATFGSRNTFDLMTQPNIKRPEDLRGKRIGLTSIGGTTWMALLLWLEHFGLDV
                     QRDKMQLQAMGEQALTVQSLETGVVNAAILDGIFSRRLKSKGFNTLGEYSELKHQFIS
                     QALVVQRSFLQQRSDSLENLLKAEIEGLAYVLAPKNKPAVIRTLMRRLKTDAAAAEEG
                     YLDLLRGMDRKPLPTVESLVHVQRLMKVQNPKIADVKLDDINDGRLIKKLDDSGFIDK
                     AYAAQGMKP"
     gene            1834..2871
                     /locus_tag="VE170_09400"
     CDS             1834..2871
                     /locus_tag="VE170_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15693.1"
                     /translation="MRNARFIRKFCCISILGFLPFVVPTLVGAAAAPTRLVIGYASTT
                     PRLMPLWMARDQGFFAKYGIESEPVLLRSGATLVTGMASGDIQIGRTAGAAVLSAVAA
                     GHDIKMLATFSSRNSYDVVTRPHIKRAEDLRGKKLAINSIGGGTWIGAMLWLEHFGLD
                     LQRDQILLQTIGDQGMQSQALESGVVDTVFVDSVYSKLLKQKGMNIIAESHELKQPLV
                     SQSTVMPHNFLRQHPDVAEGYIKGEIEGIAFATAPKNKAAVIKTLMRRLRVDATTAEE
                     GYSDMLRGVDRKPFASLEGMRNLHRLLKPRNPKIADVKVEDVVDNRIMRKLEDSGFID
                     KIFAAHGASFK"
     gene            2964..>3801
                     /locus_tag="VE170_09405"
     CDS             2964..>3801
                     /locus_tag="VE170_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15694.1"
                     /translation="MKSSMQIVSGKLAVVIFTAISMLAFGVYNARAAAAPAKQVVGYA
                     TFTARIVPLWLAQEQGFFTKYGIDVEPVFIRGAPTLVAGLAAGSMHIGRTGGSAMLAA
                     VAAGHDFKVVAAFNTRNTYDLVVRPNIKRAEDLRGKTFGITSIGGTSWMGVILWLEHL
                     GLDEKRDNIRFQVIGDQSVQVQCVETGLCDAIAVDGVYTKQAKNRGMSVLGEYTQLKS
                     LLIGQSMVVPTAMLQQRSDVAEAYLKGEIEALVFSLAPRNKPVVLKTLSKWLKVDGSG
                     AED"
BASE COUNT          855 a         1092 c         1096 g          758 t
ORIGIN      
        1 cgcatcggcc tgaccagcat cggcggcacc acttggatgg cgttgttgct ctggctcgaa
       61 cacttcggcc tcgatgtcca gcgcgataag atgcagctcc aggcgttggg cgaacaagcg
      121 atcaccgtgc agtccctgga ggccggtatc gtcaatgcgg cgatcttcga agggattttc
      181 agccgacgac tcaaggccaa agggtttaac acgctgggcg aatactcggc gctcaaacac
      241 caatttctca gccaagccct ggtggtgcag cgcagttttt tacagcagcg cagcgatacg
      301 ttggaaaatc tactgaaggc ggaaatcgaa gcagtcgccc acgtattggc gccaaagaat
      361 aagcccgcaa tgatcaaaac tttgatgcgg cgcatgaaga cggacgcaac agcggccgaa
      421 gagggctatc tcgacctgct gcgtgccatg gaacgcaagc ctgtgccgac ggtcgaaagc
      481 atgatccatg tgcagcgcct gatgaaaata cagaatccta aaatcggcga cgtcaagctc
      541 gatgacatca acgacgcccg cttgatcaag aaactcgacg acagcggctt catcgacaac
      601 gcctatgcag cgcagggaat cagtgcgaaa cagtagcggc aatttggctg attggactct
      661 tgcgccgcga actgcgagag gatgaacttt taaacatgta cctccgtgga gaatattcaa
      721 tgaaacacct tcgccgacta actccattgt tcctgctggc tttgggcttg acggcgttac
      781 tttggagcct cccagccttc ggtgccagcg ctcccgggaa aatcattctc ggctatgctg
      841 cccccggcgc tcgcgccctg cccttctggg tggcccagga tcttggacta ttcaataaat
      901 atggggtcga tgtcgaaccg gtttttattc gcggcgcgcc gattctcgtc gccggcctgg
      961 catcgggcga tattcacgtc ggcagcaccg gcggcagcgc taccttggcg gcggtcgccg
     1021 gcgggcagga cttgaaaatc atcgccacct tcggcagccg caacaccttc gatttgatga
     1081 ctcaacccaa catcaagcga ccggaggacc tgcgcggcaa acgcatcggc ctgaccagca
     1141 tcggcggcac cacttggatg gcgttgttgc tctggctcga acacttcgga ctcgacgtcc
     1201 agcgcgataa gatgcagctc caagctatgg gcgaacaggc gctcaccgtg cagtctttgg
     1261 aaaccggcgt ggtcaatgcg gcgatcttag acggcatttt cagccggcga ctcaagtcca
     1321 aaggatttaa cacgctgggc gaatattccg agctcaaaca tcaattcatc agccaagccc
     1381 tggtggtgca acgcagcttt ttacaacaac gcagcgacag tctggaaaac ctgctgaagg
     1441 ccgaaataga gggtctagcc tacgtcttgg cgcccaagaa caaaccggcg gtgatcagaa
     1501 ctttgatgcg gcgcctgaag acggatgcgg cggcggcgga agagggttat ctcgatctgc
     1561 tgcgcggcat ggatcgcaaa ccgctgccga cggtggaaag cctggttcat gtgcagcgct
     1621 tgatgaaggt tcagaatccc aaaatcgccg acgtcaagct cgatgacatc aacgatgggc
     1681 gcttgatcaa gaagctcgac gacagcggct tcatcgacaa ggcctatgcg gcgcagggga
     1741 tgaaaccgtg ataatttgtt caaggttcaa gggttcaaaa gttcaaggtc ggacagggga
     1801 taaattcgac agtttgcaag gagaaacatt tccatgcgca atgctagatt tatacgaaag
     1861 ttttgctgca tctcgattct cggattcttg cctttcgtcg ttcccacgct ggtgggtgcc
     1921 gccgctgcgc caaccagact tgtcatcggt tacgcgtcga ccacgccgcg gctaatgccg
     1981 ctttggatgg cgcgcgatca aggcttcttc gccaaatacg gcatcgagtc ggaaccggtg
     2041 ctcttgcgca gcggcgcgac gttggtgacc ggcatggcgt caggcgatat ccaaatcggc
     2101 cgcaccgcgg gggctgcggt tctgtccgcg gtggcggccg gtcatgacat caagatgctg
     2161 gcgacgtttt ccagccgcaa ctcctacgac gtcgtcaccc gtccccacat caaacgagcc
     2221 gaagatctgc gcggcaaaaa gctcgccatc aacagcatcg gcggcggcac atggatcggc
     2281 gccatgttgt ggctcgaaca tttcggtctc gacctgcagc gcgaccagat cctgctacaa
     2341 acgatcggcg atcaaggcat gcagtcccag gccttggaat ccggcgtcgt cgatacggtt
     2401 ttcgtcgata gcgtctatag taaactgtta aagcaaaaag gcatgaacat catcgccgag
     2461 tcgcacgaat tgaaacagcc gctggtcagc cagtcgacgg tcatgcccca taactttctg
     2521 cgccagcacc cggacgtcgc cgagggttac ataaaaggcg agatcgaggg catcgccttc
     2581 gccaccgcgc cgaaaaataa agcggcggtg atcaaaactc tgatgcgccg gctgcgtgtt
     2641 gacgccacca ccgccgaaga gggctacagc gacatgttgc gcggtgtcga ccgcaaaccg
     2701 ttcgcctcgt tggaaggcat gcgcaatctg catcggctgc tgaaaccgcg aaatcccaag
     2761 atcgccgacg tgaaagtcga agacgtggtc gacaaccgga tcatgcgcaa gctggaagat
     2821 tccggcttca tcgacaaaat attcgccgcc catggcgcta gcttcaagta ggagggatcg
     2881 gccgccctcg attggcttgc gactttgatc tttgcactgc cagctgatag ctggttgacg
     2941 acggtgaacg gaggctggtg tctatgaaga gttcaatgca aatcgtctcg ggcaaacttg
     3001 ccgtggttat tttcactgcc atctcgatgc tcgccttcgg cgtttataac gcgcgcgccg
     3061 cggcggcgcc ggccaagcaa gtcgtcggtt atgccacctt caccgcgcgc atcgtgccgc
     3121 tctggctcgc gcaggagcaa ggctttttca ccaagtacgg tatcgatgtc gaaccggtat
     3181 ttatccgcgg cgcacccacc ttggtcgccg ggctggcggc cggcagcatg cacatcggcc
     3241 gcaccggcgg cagcgccatg ctggccgcag tggccgccgg ccatgatttc aaagtcgtcg
     3301 cggctttcaa cacgcgcaat acctatgacc tggtcgtccg gcccaacatc aagcgcgccg
     3361 aagatttgcg cggtaagacc ttcggtatta ccagtatcgg cggcaccagc tggatgggtg
     3421 tgatcctgtg gctggagcac ttaggtctgg acgaaaagcg cgacaacatt cgtttccagg
     3481 tcatcggcga tcaaagcgtc caggtccaat gcgtcgagac cggcctatgc gacgccattg
     3541 ccgtcgacgg cgtctatacc aaacaggcaa aaaaccgcgg catgagcgtg ctcggagagt
     3601 acacgcaact taaatctctg ttgatcggcc aatcgatggt ggtgccgacc gcgatgctgc
     3661 aacagcgttc ggatgtcgcc gaagcctatc ttaaaggcga gatcgaagcg ttagtctttt
     3721 ccctggcgcc gagaaacaaa ccggtggtgc ttaaaacatt gtcgaaatgg ctcaaggtcg
     3781 acggctccgg cgcggaggat g
//
LOCUS       DASQKS010000361         7077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1862252, whole genome shotgun sequence.
ACCESSION   DASQKS010000361 DASQKS010000000
VERSION     DASQKS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862252"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VE170_09410"
     CDS             <1..546
                     /locus_tag="VE170_09410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ15695.1"
                     /translation="LGIPPGAQVLEVGAGTGTSFPAYPTHCNVTGIDLAPDMLARARQ
                     KIADNGWRHLKVIEMDALDLKFPDNSFDYVMAFHVVTVVPDPVRMIAEVKRVCKPSGK
                     IVIVNHFTSDVPVLGTLTRSMDPLTRWLGWRTDLQLKPFIATTGLIIEKVYKINKGSL
                     YTVVLARKDSNSNGHYAAREL"
     gene            complement(727..918)
                     /locus_tag="VE170_09415"
     CDS             complement(727..918)
                     /locus_tag="VE170_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYJ15696.1"
                     /translation="MFGLGVPELMVILVIALVIFGPSKLPQIGSGLGKAIRDFKKGVT
                     EKDDEAEKEVKKDPKDLPR"
     gene            complement(927..2288)
                     /locus_tag="VE170_09420"
     CDS             complement(927..2288)
                     /locus_tag="VE170_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HYJ15697.1"
                     /translation="MLPLGEAQAANLGALDGNRPPSFASIAKKTMPVVVNISTLTQRS
                     PRSGSNDPIEEFFNRFFGESAPRENNSRSLGSGILISRDGEILTSYHVVRSADTIKVK
                     LADQSEHEARLIGKDDRTDLALIKIRRSGGNLPFAKLGNSSQLDVGDWVMAIGNPFGL
                     EHTVTAGIVSAKGRVIGAGPYDNFIQTDASINPGNSGGPLISADGEVVGVNTAIFSQS
                     GGNVGIGFAIPIDLAKKVLDQLRKNGKVVRGWLGVRAQDLNTTQAASLGVARTGADLA
                     IVTEVADKSPAAEAGIKSGDVIVEFNGKAVPKSNEFPGVIADIAPGQKVSLKILREKK
                     EQTVAVRIGELSDEADVNQQVEARDVELGLRVQRITPEAARRLGLSSAKGVLVVEVQP
                     GSPADQVGIEPADVIREVNQRAVANVKDFERATRQGRRGDRILLLVQRGDNAVFFAVK
                     RKA"
     gene            2507..3859
                     /gene="mgtE"
                     /locus_tag="VE170_09425"
     CDS             2507..3859
                     /gene="mgtE"
                     /locus_tag="VE170_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYJ15698.1"
                     /translation="MKPAPIDYDTVREMLRTATPDRVLRFIGKSHPADIALLFKGLDS
                     AEVRQLFDVLFSSRRAAKTLKELPLELLPDILALIDDEKVGRLIVRADPDDAVTFIDC
                     LPDERREQVMGLLDPERRAAVREIINYPEGTVGRLMTTEFMALPPDATAQGAIDKIRE
                     RGELESFFYLYVVDSNGKLVGVVPIRNLVIAPPARKLSEMMIADPIKADVFMDQEDAA
                     RIVAKYELLALPIVDEGGRLEGIITVDDVIDIIKEESTEDMYKMVGLAEEDRVFTPVS
                     RSVKMRLPWTFLNLLTATLAASIVGLFEGTLHEIVTLVTFMPVIAGVGGNGATQTATV
                     IIRAIALGELEFSSAWKAVVKQVSVNICIAIAAGAMIAVAAILWKGNPYLGLVLAAAM
                     LLNLGLMAGFAGAVIPLLLKGMKLDPALGSGIIVTGLTDAFGFLSFLGLATIFRSYLI
                     "
     gene            complement(3856..4827)
                     /locus_tag="VE170_09430"
     CDS             complement(3856..4827)
                     /locus_tag="VE170_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYJ15699.1"
                     /translation="MKAKPILTLLVKLLVSGGLLAYFLSQVHPERFLHAFATANYWWI
                     ALALVIYLCTQAISAARWAALARPLGIETQFTSLLKYYLIGMFFNLFAPGTVGGDVTR
                     ILYLSRDQESHANGRTVTTTHAAMSVLMDRAIGMIVLVWLGAAGLLLFPEYAVPQTVR
                     SITLLLAAGFVGAVFLLPLLRHVLPEDGPPMLVKIRLALHSYRTQWRALAVAAGLSLV
                     AHLIQAWMHLVMARALGLEVPLAFCIIVYPLVGTFSAIPVSLNGLGLREGGYIYMLAV
                     IGIGSENGIAFGLLLFLIVVIDSLIGGVLFLLQKSPKEANVIAEKTI"
     gene            complement(4824..5486)
                     /gene="purN"
                     /locus_tag="VE170_09435"
     CDS             complement(4824..5486)
                     /gene="purN"
                     /locus_tag="VE170_09435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYJ15700.1"
                     /translation="MARQVPIAVLLSGGGTNLQAIIDAIEAKKLDAKIELVLSNKSDS
                     FGLVRAQNHRIPTEVLDHKAYPSREAYDQAAVDLLGGRGVELVVLAGFMRLLSPVFIK
                     AYSNRIINIHPSLLPVFPGLNVQKKAVEHGVRFAGCTVHFVNEECDEGPIIIQAVVPV
                     FSDDTEESLAARILKQEHRIFPRAIQLYAEGRLHVSGRRVLVDGPNQDDGQVLIQPPL
                     DR"
     gene            complement(5493..6536)
                     /gene="purM"
                     /locus_tag="VE170_09440"
     CDS             complement(5493..6536)
                     /gene="purM"
                     /locus_tag="VE170_09440"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HYJ15701.1"
                     /translation="MATPKGLTYAQAGVSIEAGAELVRRIGPIAKKTKVPGVLAGVGG
                     FSALFDLKSRGYRDPVLVSSTDGVGTKLKIAFQTGVHDTIGIDLVAMGVNDILTQGAT
                     PLFFLDYFVCGKLDVKITEAVVSGIAAGCRQAGCALIGGETAEHPGDFPNGEYDLAGF
                     VVGALEKKKIIDAAAVTPGDVLIGIPASGLHSNGYSLARKVLLERGGLKLNRRIPELG
                     RNLGEELLEPTRIYAKIAKKLFGAFPIKGAAHITGGGISGNLPRVLPPGRHAVVERSS
                     WPVPPVFGLIQKIGSVTQPEMEATFNMGLGMIFVVDRKDADPVVRMLRRIGERSFVVG
                     EIRQGAKGATIRS"
     gene            complement(6619..>7077)
                     /locus_tag="VE170_09445"
     CDS             complement(6619..>7077)
                     /locus_tag="VE170_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ15702.1"
                     /translation="TDKARALIKLVGLEGFEEMYPHELSGGMQQRASLCRALVTDPQL
                     LLMDEPFGALDAMTRDELDMELLRIWEGRKKTVLFVTHSIQEAVFLSDLVFVMSARPG
                     RLLETIAIDLPRPRTMEMMSTVKFGEYTLTIRALLASAGGSASAAIGSTM"
BASE COUNT         1524 a         2137 c         1948 g         1468 t
ORIGIN      
        1 ctcggcatcc ctccaggcgc gcaggtgctc gaagtcggcg ccggcaccgg cacttcgttt
       61 ccagcctatc ccacccattg caatgttact ggcatagact tggcgccgga catgctcgcg
      121 cgggcgcggc aaaaaatcgc cgacaacggc tggcgccatc taaaagtaat cgagatggat
      181 gccctcgatc tcaagtttcc cgacaacagc ttcgattatg tcatggcgtt tcatgtcgtc
      241 accgtggttc ccgatccggt gcgtatgatt gctgaggtca aacgggtgtg caagccaagc
      301 ggcaagatcg ttatcgtcaa tcacttcacc agcgacgttc cggtactcgg cacgctcact
      361 cgatccatgg atccgctcac ccgttggctc ggttggcgca ccgatctcca gcttaaaccg
      421 ttcatagcaa ccaccggcct catcattgaa aaggtttata aaattaacaa gggttccctc
      481 tacaccgtgg tattggccag aaaagactcc aactccaacg gtcactacgc cgcgcgtgaa
      541 ctctagccta gacgcgcatg gacgcggcaa atcgatggct ttagcgcgga ctgcgccgtc
      601 aaaatcgact gacacttttc tctggaacca aacgaatgcg gttagcgatt gtctccggtg
      661 agcggcgagc aatcgttatc ggacgtggac tggcggcaat tcacgaggcg gcggtcgtct
      721 gacggcttat cgcggcaggt ctttgggatc ctttttcact tctttctcag cttcgtcatc
      781 tttttcggta acgccttttt tgaagtcccg gatcgccttg ccgagtccgc tgccgatctg
      841 cggcagcttg ctcggaccga agatcaccag ggcgatcacg agaatcacca tcagttccgg
      901 cacaccaagt ccaaacatga ttcctcctaa gccttccgct tcacggcgaa gaataccgcg
      961 ttatcgccac gctgcacaag cagcaagatg cggtcgccac gccggccttg ccgcgtcgcc
     1021 cgttcaaaat ctttgacatt ggccacggcg cgttggttaa cctcgcgaat cacgtccgct
     1081 ggttcgatgc cgacctggtc ggccgggctg cccggctgca cctcgaccac caagacccct
     1141 ttggctgaac tcaaacccaa gcggcgcgcc gcttccggcg tgatgcgttg cacccgcagc
     1201 ccgagttcga catcgcgcgc ctcaacctgc tgattgacat cggcttcgtc gctgagttcg
     1261 ccgatgcgca ccgcgacggt ttgctccttt ttctcgcgca atattttgag cgagactttt
     1321 tgccccggag cgatatcggc gatgacgccc ggaaactcat tgctctttgg cactgctttg
     1381 ccattgaatt cgacgatcac atcgccgctc ttgatgcccg cttcggccgc ggggctcttg
     1441 tcggctactt cggtgacgat cgcaagatcg gcgccggttc gcgcgacgcc gagtgaagct
     1501 gcctgagtgg tgttaagatc ctgggcgcgg actcccagcc aaccgcgcac cactttgccg
     1561 ttttttcgca actggtccaa tacctttttc gccagatcga tgggaatcgc gaaaccgatc
     1621 ccaacgttgc cgccggattg actgaagatc gccgtattga cgccgactac ttcgccgtcg
     1681 gcgctgatca gcggtccacc gctgttgccg gggttgatcg aggcatcggt ttgaatgaaa
     1741 ttgtcgtagg gaccagcgcc gattacccgc cctttggcgc tgacgattcc cgcggtgacg
     1801 gtatgttcga gaccgaaagg gttgccaatc gccatgaccc aatcgccgac gtccagctgg
     1861 ctcgaattgc caagtttggc gaagggcaaa ttgccgccgg agcgacggat cttgatcaag
     1921 gccaagtcgg tgcgatcgtc cttaccaatc aagcgcgctt catgctcgct ctgatccgcc
     1981 agtttcacct tgattgtatc ggcgctgcgc acgacatgat aactggtgag aatctcgcca
     2041 tcgcggctga ttaaaatccc cgagcccaaa ctgcgcgagt tgttctcccg cggcgctgac
     2101 tcgccgaaaa atcgattaaa gaattcttcg atgggatcgt tagagcctga acggggcgag
     2161 cgttgcgtca aagtcgatat attgaccacc accggcatgg tttttttggc gatgctggca
     2221 aagctgggag gacgattgcc gtccaaggcg cccaggttcg cggcttgggc ttcgccgagc
     2281 ggaagcaatc ctagcccgct cagcccatac aataaactgg cgatcaaata ccgccttggc
     2341 gatcgatcac gtgtaaccat cagcgataga tcctcgatag attcgattct gccagcacca
     2401 tagcaaattt cccgggcacg gcgaaaccgg cggacgaaac gcaaaacatt tgcaaataac
     2461 catctcagtg tctatgaagt aaccagcctt atcgtcgaaa gcacccatga aacccgctcc
     2521 catcgattac gacaccgtgc gtgagatgtt gcgcaccgcg acgccggacc gcgtgctgcg
     2581 tttcatcggt aaaagccacc ccgccgacat cgcgttgctg ttcaagggcc tcgattcggc
     2641 ggaagtgcgc cagctgttcg acgtgctgtt ctcgtcgcgt cgcgccgcca agactcttaa
     2701 agagctgccc ctcgaattat tacccgacat tctcgccttg atcgacgacg agaaggtcgg
     2761 ccggctgatc gtgcgcgcgg atccagacga tgcggtcacg ttcatcgact gcctccccga
     2821 cgagcgccgc gagcaggtca tgggcctgct cgacccggag cgccgagccg cggtgcggga
     2881 aatcatcaac tatcccgagg gcaccgtcgg ccgcctgatg accaccgaat tcatggccct
     2941 gccgcccgac gccaccgccc aaggcgccat cgataagatt cgcgagcgcg gtgaactcga
     3001 gagttttttc tatctttatg tcgtcgacag caacggcaaa ctggtgggtg tcgtgccgat
     3061 tcgcaatttg gtgatcgccc cgccggcgcg caaattgagc gaaatgatga tcgccgatcc
     3121 aatcaaagcc gatgttttta tggatcagga ggacgccgcc cgtatcgtcg ccaagtacga
     3181 gcttctggcg cttcccatcg tcgatgaagg cggccgcttg gaaggcatca tcaccgtcga
     3241 cgatgtcatc gacatcatca aggaagaaag caccgaggac atgtacaaga tggtcggctt
     3301 ggccgaggaa gaccgcgtgt ttacgccggt ctcgcgctcg gtcaaaatgc gcctgccttg
     3361 gaccttcctc aatctgttaa ccgctacgct cgccgcctcc atcgtcggcc tcttcgaagg
     3421 tacgctccat gaaattgtca cgttggtcac cttcatgccg gtgatcgccg gcgtcggcgg
     3481 caacggcgct acgcaaaccg cgacagtgat cattcgcgcc attgccctcg gcgagctcga
     3541 gttttcctcg gcttggaagg cggtggtcaa acaggtgtcg gtcaatatct gcatcgccat
     3601 tgccgccggc gccatgatcg ccgtggcggc aattttgtgg aagggcaatc cttacttggg
     3661 actggtactg gccgccgcga tgttactgaa tctcggactg atggcgggct ttgccggtgc
     3721 ggtcatcccc ttattgctta aaggcatgaa actcgacccg gcgctcggtt ccggcatcat
     3781 cgttaccggc cttaccgacg cttttggctt tctctcgttt ctcggactgg cgacgatctt
     3841 tcgcagttat ttgatctaga ttgtcttctc ggcaatcacg ttcgcctctt taggcgactt
     3901 ttgcagcaag aacagcacgc cgccaattaa actgtcgatg acaacgatca agaataacaa
     3961 caggccgaag gcgatgccat tttcactgcc gatgccaatc acggcaagca tatagatgta
     4021 accgccttcg cgcagcccca atccattcaa actcacgggg atcgccgaaa aggttcccac
     4081 caacggatag acgatgatac aaaaagcaag cggcacttct aaaccgagcg cgcgggccat
     4141 gactagatgc atccaggctt ggattaaatg ggcgaccagg gagaggccag ccgccaccgc
     4201 caacgcccgc cactgggtgc ggtaactgtg cagagccaag cggattttga ctagcatagg
     4261 agggccgtcc tcgggcaaca catgtctgag caacggcagc aggaaaaccg cgccaacaaa
     4321 acccgccgcg agtaacaacg taatcgaacg aaccgtctgc ggaaccgcgt attcgggaaa
     4381 taacagcaac cctgctgcgc ctaaccacac caatacgatc ataccgatgg cgcgatccat
     4441 cagcaccgac atggcggcgt gcgtggtcgt cacagtgcgc ccattggcgt gggattcttg
     4501 atcgcgcgag aggtaaagaa tccgcgtgac atcgccgccc accgtaccag gcgcgaacaa
     4561 attgaagaac attccaatca agtaatattt gagtaaacta gtgaactgcg tctcgattcc
     4621 caacggccgc gcgagggcgg cccagcgcgc ggcactaatc gcttgggtac acagatagat
     4681 gaccagcgcg agcgcgatcc accaataatt tgccgtcgca aaggcatgca gaaaccgctc
     4741 gggatgaacc tgcgacagaa aataagccaa caggccgccg ctgacgagta gcttcactaa
     4801 caaggtgaga atcggtttgg ccttcatcgg tccagcggcg gttggatcag cacctggccg
     4861 tcatcctgat taggaccatc gacgagcacc cggcggccgc tgacatgcag gcgaccctcg
     4921 gcgtaaagct ggatggcacg gggaaaaatt cgatgttctt gcttgagaat ccgcgccgcc
     4981 agtgattctt ccgtatcgtc ggaaaaaacc ggcacgaccg cttggatgat gatcggccct
     5041 tcgtcgcact cttcgttgac aaaatgcacc gtgcagccgg caaagcgcac accatgttcg
     5101 acggctttct tctgcacatt gagaccggga aataccggca gcaaagacgg gtggatgttt
     5161 ataattcgat tggagtaggc cttgatgaaa acaggcgaga gcaaacgcat aaagccggcg
     5221 agcaccacta gctcgacgcc gcgtccgccc agcaggtcga ctgccgcttg atcgtaggct
     5281 tcacggctcg gatacgcctt gtggtccaag acttcggtag ggatgcggtg attttgcgcc
     5341 cgcaccaaac cgaaagagtc ggatttgttg ctcaaaacca attcgatttt agcatcgagt
     5401 tttttcgcct caatggcgtc gataatcgcc tgtagattgg taccgccgcc ggagagcaaa
     5461 accgcgatag gaacttgtct tgccatacta tttcacgaac gaatcgttgc gcccttcgcg
     5521 ccttggcgaa tttcaccgac gacgaaactt cgctcgccaa tgcgtctcag cattcggacc
     5581 accggatcgg cgtccttcct gtccacgacg aaaatcatgc ccagacccat attgaatgtc
     5641 gcctccatct ccggctgggt gaccgagccg attttttgaa tcaagccgaa gaccggcggc
     5701 accggccaac ttgaccgctc aaccaccgca tgccgccccg gcggcaatac ccgcggcaga
     5761 ttgccgctga taccgccgcc ggtgatatgc gctgcgcctt tgatgggaaa ggcgccgaac
     5821 agtttcttgg caattttagc ataaatgcgc gtcggttcga gcaattcttc gccgaggttg
     5881 cgaccgagct cgggaatgcg ccggtttaat ttcaatccgc cccgttcgag cagaaccttg
     5941 cgcgccagcg agtaaccatt gctgtgcagt ccgctggcgg ggatgccgat caagacatcg
     6001 ccaggcgtga ccgctgcggc atcgataatt ttctttttct ccaaagcgcc gacaacaaag
     6061 ccggcgaggt cgtactctcc gttaggaaaa tcaccgggat gctcggcggt ttcgccgccg
     6121 atcagcgcac aaccggcctg gcggcagccg gcggcgatgc cggacaccac cgcttcggtg
     6181 attttcacgt cgagcttgcc gcagacgaag tagtcgagaa aaaaaagcgg cgtcgcgccc
     6241 tgggtcaaga tatcgttgac ccccatagcg accaaatcaa tcccgatagt gtcatgaacg
     6301 ccggtctgga aagcgatctt cagcttggtg ccgacaccgt cggtggacga gaccaggacc
     6361 ggatcgcgat agccgcgcga tttaagatcg aagagcgcgc tgaagccgcc gacaccggcg
     6421 aggaccccgg ggaccttggt tttcttggcg attgggccga tgcgccgcac gagttcagcg
     6481 ccggcttcga tgctaacgcc agcctgggcg taagtgagac cttttggagt tgccattcga
     6541 tgcggattaa ttttcggatt cgtcggggcg tattacgctg cgcgcctgcc gccatcatag
     6601 cgcactgaaa tacgaaattc acatggtcga gccgattgcc gcgctggcac tcccaccggc
     6661 cgaggcgagt aaagcgcgaa tggtcagcgt gtattcaccg aacttgaccg tactcatcat
     6721 ttccatggtg cgtggccgag gtaaatcgat ggcaatcgtt tccaacaagc gccccggtcg
     6781 cgccgacatg acgaaaacca ggtcggagag aaacaccgct tcttgaatgc tgtgcgtcac
     6841 gaacaggacg gtctttttgc gcccttccca gatgcgcagg agctccatgt ccaactcgtc
     6901 gcgggtcatg gcatccagcg cgccgaaggg ttcgtccatc agcaacagct gcgggtcggt
     6961 caccagcgcg cggcatagcg aagcgcgctg ctgcatgccg ccggagagct catggggata
     7021 catctcttcg aaaccctcta aaccgacaag cttgatcaag gcgcgcgcct tgtcggt
//
LOCUS       DASQKS010000362         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1451055, whole genome shotgun sequence.
ACCESSION   DASQKS010000362 DASQKS010000000
VERSION     DASQKS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451055"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VE170_09450"
     CDS             complement(<1..465)
                     /locus_tag="VE170_09450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HYJ15703.1"
                     /translation="MRLSVLDQSTTSKDKSQDTAIRETLDLARHCDALGYHRYWVSEH
                     HNSGNIVGTAPEILMAAIAATTPRIRIGSAGVMLPHYSALKVAEQFRVLEAIAPGRID
                     LGIGRAPGSDQLTSYALNPNPQNVQDQFPRQVQELQHWVSGKPLPEGHPFQKI"
     gene            complement(619..1533)
                     /locus_tag="VE170_09455"
     CDS             complement(619..1533)
                     /locus_tag="VE170_09455"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYJ15704.1"
                     /translation="MRIILIGQAAFAEKTLEKLAGKGEEVVAVYCPPDPPSGKFDPVK
                     QKAIDLQIPVRQHKSFKAPEVFDEFVALHADLAILAFVTQIVPQQIFSAPSLGSICFH
                     PSLLPKYRGASAINWALINGEAITGLSLFWVDKGIDTGPILLQKEVTVEPNDTTGSLY
                     FNKIFPLGVEAIGEAVDLIKTGNPPRIVQDESKANYDPICGDEHAKIDWAKPAQQVYN
                     LIRGCDPQPGAHTTFTDKRLRLFDARMHLANNSATAGQVTGMGAEEITVALNGGTLTV
                     KKVRGEGAKTNAADFAQQIGLKVGDKLG"
     gene            complement(1592..>2122)
                     /locus_tag="VE170_09460"
     CDS             complement(1592..>2122)
                     /locus_tag="VE170_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HYJ15705.1"
                     /translation="FMLIEYKGKRPNVSPTAFIAPTAVLIGDVTVGDESSIWWGAVLR
                     ADLGGYPIVIGARTSIQDNCVIHSGEMATRVGDDCTVGHGAIMEGCDIKRGAIIGMNC
                     TILENTEIGEGSLIAAGAVVVPHTKIPPHVLAAGTPAKVKKEIEGEARKWIEMGATTY
                     TGLCKSYLGGDYKIVD"
BASE COUNT          396 a          574 c          653 g          499 t
ORIGIN      
        1 gattttctga aacgggtgac cctcgggcag aggcttccca gatacccagt gttgcagctc
       61 ctggacttgc cggggaaatt ggtcctgaac gttctgcgga ttgggattga gcgcgtagga
      121 cgtcaactga tccgagcccg gcgcgcggcc gattcccaag tcgatgcggc ccggggcaat
      181 ggcttccagg acacggaact gctcggcaac tttgagcgcc gaatagtgcg gcagcataac
      241 gccggcgctg ccgatgcgga tgcgcggcgt ggtggcggcg atcgccgcca tgaggatctc
      301 gggggcggtg ccgacgatgt taccggagtt gtgatgctcc gagacccagt agcgatggta
      361 acccagggcg tcgcaatggc gcgcgagatc gagggtctca cggatcgccg tatcctgcga
      421 tttatccttg gacgtcgtcg attgatcgag tactgacaat ctcatggaag tttgctttca
      481 gacaaacccg tattttatgc gaaacgtcgg caggatacca tgcgggccaa atgttaggtc
      541 ctagcgaagc gattcttcat tccttaacac cgggtggtgt tcaagtaacc agtggtgatc
      601 gggttcgtgt gcggctcttc acccgagctt atcgccgacc tttaggccaa tttgctgggc
      661 aaaatccgca gcgttggttt tggcgccctc gccgcggact ttttttaccg ttagcgtacc
      721 gccgttgagc gcgacggtaa tttcttcggc acccattccg gtgacctgcc ccgccgttgc
      781 tgaattattt gccaagtgca tgcgggcatc gaagagccgt agccgtttgt cggtgaatgt
      841 cgtatgagcc ccgggctgcg ggtcgcagcc gcggattagg ttataaactt gctgagcagg
      901 tttagcccag tcgatctttg catgctcgtc gccgcagatc gggtcataat tggctttcga
      961 ttcgtcttga acgatgcgcg gcgggttgcc ggtcttgatc agatcgacgg cttcgccgat
     1021 ggcttcgaca ccgagcggga agattttgtt gaagtacaat gagccggtgg tgtcatttgg
     1081 ctctactgtc acttcttttt gcaacaggat cggtccggta tcgatgcctt tgtcgaccca
     1141 aaaaagcgaa agtccggtga ttgcctcgcc gttgatcaac gcccaattga tggcgctggc
     1201 gccgcgatat ttcggcaaaa gcgacggatg aaagcagatg ctgccgaggc tgggcgcgct
     1261 gaaaatttgc tgcgggacga tctgggtgac gaaagcgagg atcgctagat cggcatgcaa
     1321 cgcgacgaat tcgtcaaaca cttccggcgc tttgaacgat ttatgctggc gcaccgggat
     1381 ctgaagatcg atggctttct gttttaccgg atcgaatttg ccgctgggcg gatcgggcgg
     1441 gcaatagacc gcgaccactt cttcgccttt gccggcgagt ttttccagag tcttttcggc
     1501 gaacgccgcc tgtccgatca gtattatgcg cattggatgg aactccgatt cgctagagct
     1561 ggagccttaa gacacgagca tggtgattta tttagtcgac gatcttgtag tcaccgccca
     1621 gatagctctt gcacaggcca gtataggtgg tcgcgcccat ctcgatccat ttgcgcgctt
     1681 ccccttcgat ttctttttta actttcgccg gcgtgccggc ggccagcaca tggggcggga
     1741 tcttggtgtg cggcacgacg acggcgccgg cggcgatcag cgagccttcg ccgatctcgg
     1801 tgttctccaa gatcgtgcaa ttcatgccga tgatggcgcc gcgcttgata tcgcagcctt
     1861 ccatgatcgc gccatggccg acggtgcagt cgtcgccgac ccgggtggcc atctcgccgg
     1921 agtgaatcac gcagttatct tgaatgctgg tgcgggcgcc gatgacgatg ggatagccgc
     1981 ccaggtcggc gcgcaatacc gcgccccacc agatgctgga ttcgtcgccg acggtgacat
     2041 cgccgatcaa gaccgcggtt ggcgcgatga atgccgtggg cgaaacattg ggccgtttgc
     2101 ctttgtattc gatgagcatg aa
//
LOCUS       DASQKS010000363         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3557369, whole genome shotgun sequence.
ACCESSION   DASQKS010000363 DASQKS010000000
VERSION     DASQKS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3557369"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VE170_09465"
     CDS             complement(<1..718)
                     /locus_tag="VE170_09465"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYJ15706.1"
                     /translation="MLYEAEQGDFSIMLLGDVMPTRRLAVFGEERYLKLREICLSADA
                     TFANLETAVHRYLEGHQNISGGTYMTTEPQLLDDLKWLGVNLVSTANNHAFDYGEDGI
                     MATCGYLDAAGIVHAGTGRHLREARAPAYLDTKRGRVALIGVTATMPGHAVAGAQRPD
                     TPGRAGVNPLRHSLTHVVDQRGLDELRRLGTALGVDAARTRRENLGDPHARGGVESAS
                     EYSFGNMRFEKGESFGLRTIG"
     gene            complement(726..1109)
                     /locus_tag="VE170_09470"
     CDS             complement(726..1109)
                     /locus_tag="VE170_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15707.1"
                     /translation="MRHIALKVRDLARAKRFYQEVLGMNVVWEPDDKNVYLSSGCDNL
                     ALHEVADSLARSGDERQLDHLGFIVESIDRVRELEQDFVRQGVVLVHPFKIHRDGSAS
                     FYCADADGTVIQMLYEPTLSSQKIS"
     gene            1274..>2641
                     /locus_tag="VE170_09475"
     CDS             1274..>2641
                     /locus_tag="VE170_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HYJ15708.1"
                     /translation="MSKNTKIARTTYPHLGILADISTLITHSHDLQDTLDQLVATVAE
                     RMQTEVCSLYIFDAKKNRLILVATMGLDPESIGKVSMATGEGLTGLVIEQMKPVMVVD
                     ALAHKRYKYFPETHEEHFHSFLGVPLLEKRNPLGVLVVQTSRRREFSRDEIRLLTTIS
                     GQAASIILQARLTDNLRSKEEERKQYQKRMNDAMRKLRSYESMPPETSAKTKQKWRGR
                     LIGLAASPGFGRGQAFVLEPRMEMNAIKKKKSRNPKHEIERFRGAVERGIDQISVIKR
                     RMSELISAEEGAIFDVYRLILEDPQIIHQIEQQIRKGGFTAEYAVRVVFERYMESIAK
                     LDNNYLRERTGDVKDAAQRLLETLSGGDGQQYDVPDDAILVAQDLSPADLSMLEGDKF
                     RGIVLATGGVTSHASILAKSFEIPSVVAVEDVMERVRQGDQLIIDGNAGSLHVNPTAE
                     VINEYD"
BASE COUNT          603 a          773 c          728 g          537 t
ORIGIN      
        1 gcccgatcgt gcgtaggccg aagctttcgc ctttttcaaa gcgcatattg ccgaaactgt
       61 attcgctcgc gctctccaca ccgccgcgcg cgtgagggtc gccgagattc tcacggcgcg
      121 tgcgcgccgc gtcaacaccc agcgccgtgc ccaagcggcg gagctcatca agaccgcgct
      181 gatcgacgac gtgagtcaag ctgtggcgca gagggttgac tccggctcgt cccggcgtgt
      241 ccggccgctg cgcgccagcg acagcgtggc cgggcatggt cgcggtcaca ccgatcaagg
      301 caacccgacc gcgtttcgta tcgagatatg ccggcgcgcg cgcctcgcgc aagtgtctgc
      361 cggtgccggc gtgcacgatg ccggcggcat ccaaataacc gcaagtcgcc atgatgccat
      421 cttcgccgta atcgaaggcg tgattattgg cggtcgaaac cagattgacg cccagccatt
      481 ttaagtcgtc aagcagctgc ggctccgtgg tcatgtaagt accgccgctg atattctggt
      541 gcccttcgag gtagcgatgc acggcggttt ccaagttggc gaaagtggcg tcggcactga
      601 ggcaaatttc ccgcagtttt aagtaccgct cctcgccaaa taccgccaag cgccgcgttg
      661 gcatgacgtc gcccaacaac attatcgaaa aatcgccctg ttcggcctcg tagagcataa
      721 caccctcagc tgattttttg tgaactcaag gtaggctcgt agagcatttg aatgaccgtg
      781 ccgtcggcgt cggcacagta aaacgacgcg ctgccgtcgc gatgaatttt gaacggatgg
      841 accaggacga cgccttggcg cacgaagtct tgttccaatt cgcgcacccg gtcgatgctc
      901 tcgacgatga aaccgagatg atcgagctgt cgttcgtcac cagatcgagc caagctatcg
      961 gcaacttcat ggagcgccag attatcgcag ccggaggaaa gataaacgtt cttgtcatcc
     1021 ggctcccaga ctacgttcat gccgagcact tcttgataaa atcgtttcgc gcgcgcgaga
     1081 tcgcgcactt tgagtgcgat atggcgcata ccccgtaagc cggcaatctg actcatagcc
     1141 ggcatatata tcatacgacg gtgaaaaatg tagtttacgc gtggcccggt gcggtggctg
     1201 gtccgacaac tccaactgcc tggcgaatca gtgaaattta tgttttatag aaaactctat
     1261 ctgcgtcgct gccatgtcca agaacaccaa gatcgctcgc accacctacc cccacctggg
     1321 catactcgcg gacatcagca cactaattac ccattcccac gacctgcaag acaccttgga
     1381 ccagctggtc gccacagtcg ccgaacgcat gcagaccgaa gtatgttcgc tatatatttt
     1441 cgatgccaag aaaaatcgct tgattcttgt agcgaccatg ggccttgacc ccgagtccat
     1501 cggcaaagtg tccatggcca cgggtgaagg gttgaccggt ttagtcatcg agcaaatgaa
     1561 gccggtgatg gtggtcgatg cccttgccca taagcgttac aaatattttc cggaaaccca
     1621 cgaggaacac ttccactcgt tcctcggcgt gccgctgttg gagaagagaa accctttagg
     1681 ggtgctggtc gtgcaaactt cgcgccggcg tgagttttct cgcgacgaaa tccgcctact
     1741 gaccaccata tcggggcaag cggccagcat cattctccag gctcggctca ccgacaacct
     1801 ccgctccaaa gaagaggagc gcaagcaata ccagaagcgc atgaatgacg ccatgcgcaa
     1861 gctgcgctcc tatgaaagca tgccgccaga gacctcggca aagaccaagc agaaatggcg
     1921 cggccgattg attggattgg cggcgtcgcc tggcttcggc cgcggccaag cgttcgtcct
     1981 agagccgcgc atggagatga acgcgattaa aaagaaaaaa tcgcgtaatc caaagcatga
     2041 aatcgagcgc ttccgtggcg ccgtggagcg cggcatcgac cagatcagtg tgattaagcg
     2101 gcgcatgagc gaactcattt ccgccgaaga gggcgcgatt ttcgacgtct atcggttgat
     2161 cctcgaagac ccgcagatca ttcaccagat cgagcaacag attcgcaaag gcggtttcac
     2221 cgcagagtat gccgtgcggg tggtcttcga gcgctacatg gaatccatcg ccaagctcga
     2281 caataactat ctgcgtgaac ggaccggcga tgtcaaagat gccgcgcagc gcttactcga
     2341 aaccctttcc ggcggtgatg gccaacaata cgatgtcccg gacgacgcga tactggttgc
     2401 ccaggatctt tcgcccgccg atttgagcat gctcgaaggt gacaaattcc gaggtatcgt
     2461 tttggctacc ggcggtgtca catcccatgc atcgatattg gccaagtcct ttgaaatccc
     2521 tagcgtggtt gcagtcgaag atgtgatgga acgggtgcgc caaggcgatc aattgatcat
     2581 cgacggcaac gccggtagct tgcacgtcaa tcccaccgcc gaggtcatca acgaatacga
     2641 t
//
LOCUS       DASQKS010000364         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_611475, whole genome shotgun sequence.
ACCESSION   DASQKS010000364 DASQKS010000000
VERSION     DASQKS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611475"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1180
                     /locus_tag="VE170_09480"
     CDS             179..1180
                     /locus_tag="VE170_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15709.1"
                     /translation="MRFAFRVSMVILLCLPGRHAVHAQDDLIAKAQSERELVVYGTAL
                     VGQFDKFIEPFKRRYPFLTPMYSRTTGEALTTKVLQEVAARQLSADAILINNYTQRIF
                     MKRNIITAYLPPTAKNFPAGFADQQGYWYGFYLVPYAITYNTSLVSKANAPKSFDDLL
                     QPKWKGQISLEREEYLVTQAHTQFLGQQKAVDYFKKLARQDLIMVNGHSKQAVLLTAG
                     EFPIIVYSDIARTEELKRKGAPVEWVRADPHVTVLVTASITREAKHPAAARLFMNYLA
                     SDEGQKAVFAMDKPPALPKFQPDYLRGLNLFPADWTLSDTFEEYNKTYREIFWGGRN"
     gene            1233..2279
                     /locus_tag="VE170_09485"
     CDS             1233..2279
                     /locus_tag="VE170_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ15710.1"
                     /translation="MLVLHFILLALLVLPSGAGAQARKPSTIAELVTYRGADREQLLY
                     AGAKSEGKIVWYTSLAGDSYKELVKAFETKYAGVKVESYRAQGADLVVRLEEESKARR
                     NIADTIETTEGSLIFLRDGKLLRPYDSPQLDRYPEDGKERADKNLVYWALARESYIGF
                     TYNKTLITKDAVPKNFDGLFHPQLKGKLGISIGQTSDKVIGAMIKSKGEEFVRKLKAQ
                     EMKLYSIDAPALVNTITTGEIAGSPAIFQTHTLSAASKGAPVEWVPMDLVPTNVGSAA
                     IAFNPPHPHAALLMADFLLSPDGQAVLEKHFYGSGAKDHGFKKWRPEHGLTTDQYEKE
                     LLRWDKLLHSITRK"
     gene            2354..3439
                     /locus_tag="VE170_09490"
     CDS             2354..3439
                     /locus_tag="VE170_09490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15711.1"
                     /translation="MNNLRIIDADGHVQERDANWQELLDPPYRKQAPSMVQCADGRTH
                     LLLEGKIWAKPSGKGCGIGTAPITRKPRTTTGMWDPAQRIKDMDMEGIDTAVNFGTTV
                     FLSLPFLEDHDLACAIAHGYNTWLAEYSKTDSKRLKGVALVAQQEPQEAAKELERCVK
                     QLGFVAVAVAGHSAGRNLDHPDLYPFYAKAEELGVPICVHVGSGRPAAAAERFDNPYF
                     VHATTHAFEQMIGVMCIVGGGILERYPKLKVAFLEAGAGWVPYWMERLDEHYEYMAPA
                     VPLMKKPPSEYMKSEQVYYSFEPDELTLNFVMDYIGEDRLVFASDYNHGDSKFPHTVE
                     SVTGRKNLTASQLRKLMCDNAARLYNI"
     gene            3471..4478
                     /locus_tag="VE170_09495"
     CDS             3471..4478
                     /locus_tag="VE170_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15712.1"
                     /translation="MKRTQSGKLLGLTLGISLLAVCNAGAQDKPLKKFRWGVTSLSAS
                     QWIPWIAKDAKLYEKHGMEAELVLLRGSGQSAQAIIGNSILGAPCTLTTVMSADLSGA
                     DLVTVAHTVAGVQSKLLVRPEIKKPEDLRGKRIAVSGFGSLGDFLERYILKKYGIDPV
                     RDVVMLSIGNQPDRIQALFTGLVDAADLSHPADIQAERKGYRILWDAKQEVAYPSMSI
                     VTRRKWVNEDRDSVMRMVKAHVEAIHYLKTNKEPSMKILGKYLKTNDRELLESSYEIY
                     RKDFISVPYPITQGLQPTYEYVAAQRPEIWQHKPEAFMDTSFIAELEKSGFIKSLTKA
                     H"
     gene            complement(4465..5268)
                     /locus_tag="VE170_09500"
     CDS             complement(4465..5268)
                     /locus_tag="VE170_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HYJ15713.1"
                     /translation="MSNKESVGSDRSTRPRRLRPSGGYRALHSFQVATVIYDFTVSFC
                     DRFIDKRSRTHDQMVQAARSGRQNIAEGSRAAATSSQTELRLVNVARASLDELLLDYE
                     DFLRQRGKRQWAKDDPAAREVRAVGLSRDPTDRSDPTDHRGKYSRWLDAADPAVVANT
                     LLCLIHQANYLLDQQIAALEKGFVQEGGYTEQLAVARLAERRRQQKDRTDPTARSDLS
                     DQTAAAPNCPACGKLMVVRTARKGELAGSQFWGCSGYPQCKGARSLSAP"
     gene            complement(5276..>5665)
                     /locus_tag="VE170_09505"
     CDS             complement(5276..>5665)
                     /locus_tag="VE170_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ15714.1"
                     /translation="ADDKFATKVGRQERRLEIDQKLSNWTAKYTAKQIFRLLQEAGVA
                     AGIPSSGEDLFHDIHLRARGHIVETEAQPWGKITHHGLPGIPSLSKADAARPAPWIGV
                     NNDQVFGEILGLSAARIEELKQREAIK"
BASE COUNT         1260 a         1704 c         1578 g         1123 t
ORIGIN      
        1 gagttggcgg ggaagaaatt ggcgggcgac tgattggcgg gggcgaaatg tttttcgctc
       61 tacccgaatc ttcggggcct gcttggggcg aattgtcggc gtgagcagcc ggtcgtcttt
      121 ctcaactcta tccaagggtg aagacgccaa agcgtgacat aaccgaaagc gaggctctat
      181 gcgattcgcc tttcgagtgt cgatggtaat tttgctttgc ctcccgggcc ggcacgcggt
      241 gcacgcccag gacgatctga tcgccaaagc gcaaagcgaa cgcgagctgg tcgtttacgg
      301 aacggctcta gtcggccagt tcgacaaatt catcgagccc ttcaagcggc gctatccgtt
      361 cctcacgccc atgtactccc gcaccacggg cgaagccctg accacgaagg tattgcagga
      421 agtagccgcc cgccagttga gcgccgacgc gatcctgatc aacaattaca cccagcgcat
      481 cttcatgaag cgcaatatca tcaccgccta cctgccgccc accgcgaaaa acttcccggc
      541 gggcttcgcc gaccagcagg gctactggta tgggttttac ctggtgccct acgcgatcac
      601 ctacaacacc tccctggtgt ccaaagccaa tgcgccgaaa tctttcgatg atttgttaca
      661 gcccaaatgg aaagggcaga tatccctcga gcgggaagaa tatctggtaa cgcaagcgca
      721 tacgcagttt cttggccagc agaaggcggt cgactatttt aagaagctgg cgcgccagga
      781 tctgatcatg gtcaacggcc acagcaaaca ggcggtgctg ctgaccgcgg gcgaatttcc
      841 catcatcgtt tacagcgata tcgccaggac cgaggagttg aagcgcaaag gcgcgccggt
      901 ggaatgggtg cgcgccgatc cccatgtcac ggtgctggtc acggcgtcga tcacccgcga
      961 agcgaaacac ccggccgcgg cgcggctgtt catgaattac ctggctagcg atgaaggaca
     1021 aaaagccgtt ttcgccatgg acaaaccgcc ggcgctgccg aagtttcagc cggactatct
     1081 gcgcgggtta aatttatttc cggcggactg gacactgtcg gatacttttg aggagtacaa
     1141 caaaacctat cgggagattt tttggggcgg taggaattaa ctcgttatta ataacggcct
     1201 ccctatattg cagcggaaag gagactccaa ccatgctggt gcttcatttc attctcttgg
     1261 ctctcttagt tcttccctcc ggcgccgggg cgcaagcgcg caagccgtcg accatcgcgg
     1321 aactagtgac ctatcgcggc gccgaccgcg agcaactgct ttacgccggc gccaagagcg
     1381 aaggcaagat cgtctggtac acatccctcg ccggcgactc atacaaggaa ttggtcaagg
     1441 cctttgaaac caagtatgcc ggcgtcaagg ttgagtccta tcgcgctcag ggcgcggatt
     1501 tggtcgtgcg cctggaagag gaaagcaaag cgcggcgcaa catcgccgac accatcgaga
     1561 ccaccgaagg cagcctgatc tttctccgcg atggcaaact cctgcgcccc tacgactcgc
     1621 cgcagctcga ccgctatccc gaagacggca aggaacgcgc cgacaaaaac ctcgtctact
     1681 gggcgctggc gcgagaatcc tatatcggct tcacctacaa caagacgctg ataaccaagg
     1741 acgccgtgcc gaaaaatttc gacggactgt tccaccccca gctcaagggc aaattgggca
     1801 tcagcatcgg ccagactagc gacaaggtca tcggcgcgat gatcaaaagc aaaggcgaag
     1861 agtttgtccg taaactaaaa gcccaggaga tgaaactcta ttccatcgac gcccccgccc
     1921 tggtcaatac cattaccacg ggcgagatcg ccggctcgcc ggcgatcttt caaactcaca
     1981 ccctgagcgc cgcatcgaaa ggcgcgccgg tggaatgggt gccgatggat ctggtcccga
     2041 ccaacgtcgg cagcgccgcc atcgccttca atccgcccca tccgcacgcg gcgttactga
     2101 tggctgattt tcttttgagc ccggacggtc aagccgtcct ggaaaaacac ttttacggca
     2161 gcggcgccaa agatcacggc tttaagaaat ggcgtcccga gcacggcctg acgacggatc
     2221 agtatgaaaa ggaactgctc cgatgggata aactgctcca ctcgatcacg cggaaataat
     2281 ataggagatc tgtcgtaggg gcgaaaaatt tttcgcccta cccgcaccaa cggtcttcga
     2341 aaggaaaccg aaaatgaaca acctacgcat catcgacgcc gacggccatg tccaggaacg
     2401 cgatgccaac tggcaggagc ttcttgaccc gccttatcgc aaacaggcgc cgagcatggt
     2461 gcagtgcgcc gatggccgta cgcatttgtt gctcgaaggt aagatctggg ccaagccaag
     2521 cggcaagggc tgcggcatcg gcactgcgcc gatcacgcgc aaaccgcgca ccaccacggg
     2581 catgtgggat cccgcccagc gcatcaaaga catggacatg gaaggcatcg ataccgcggt
     2641 caacttcggc acgacggtgt ttttgagtct gccgtttctt gaagatcatg accttgcctg
     2701 cgccatcgcc catggctata acacctggct cgctgaatat tccaagaccg attcgaagcg
     2761 tttgaagggc gtcgccctgg tcgcccagca ggagccgcag gaagccgcca aggaactcga
     2821 gcgctgtgtc aagcaactag gctttgtcgc cgttgccgtt gccggccact cggccggacg
     2881 caacctcgat catcccgacc tctatccgtt ctacgccaaa gccgaggaac tcggcgtgcc
     2941 gatctgcgtt catgttggct cgggccggcc ggcggcggcc gccgagcgtt ttgacaatcc
     3001 ttatttcgtg cacgcgacca cccacgcctt cgagcaaatg atcggcgtca tgtgcatcgt
     3061 cggcggcggc attctcgaac gctatccaaa gctcaaagtc gcgttcctcg aggccggcgc
     3121 cggttgggtg ccctactgga tggagcgctt ggacgagcat tatgaataca tggcgccggc
     3181 ggtgccgctg atgaagaaac cgccgagcga atacatgaag agcgagcagg tttactattc
     3241 cttcgagccg gacgaactga ccttgaattt tgtcatggac tacatcggcg aggaccgcct
     3301 ggtgttcgcc tcggactata atcacggcga cagcaagttt cctcacaccg tggagtccgt
     3361 gaccgggcgc aaaaatttaa ccgcaagcca attgcgcaaa ttgatgtgcg acaacgccgc
     3421 ccggctgtac aacatctaat aatcagaaac gtaaggcgcg ggagctgtct atgaagcgca
     3481 cacagagtgg caaacttttg ggcttgaccc tcggcatctc gctccttgcc gtttgcaacg
     3541 ccggtgccca ggacaaacca ctgaaaaaat ttcgctgggg cgtcacctca ttgtcggcat
     3601 cccaatggat tccgtggatc gccaaagacg ccaagcttta tgaaaagcac ggtatggaag
     3661 ccgagctcgt gctgctgcgt ggctctggac aatcggcgca ggcgattatc ggcaatagca
     3721 ttctcggcgc cccatgcaca ttgacgacgg taatgagcgc cgacctgagt ggcgccgacc
     3781 tggtcacggt ggcgcacacg gtggcaggcg tgcaaagcaa actgctcgtc aggccggaga
     3841 tcaagaagcc cgaggatctg cgcggcaagc gcatcgcggt ttcgggtttc ggttccttgg
     3901 gagattttct cgagcgctat atcctaaaaa aatacggcat cgatcccgtc cgcgacgtcg
     3961 tcatgctttc catcggcaac cagcccgacc ggattcaagc tttattcacc gggctggttg
     4021 atgccgccga cctgtctcat cccgccgata tccaagccga gcgcaaggga tatcgcattc
     4081 tctgggacgc caaacaggaa gttgcctacc cttccatgtc gatcgtcacc aggcgtaagt
     4141 gggttaacga agaccgggat tcggtcatgc gcatggtcaa agcccatgtc gaagcgattc
     4201 actatctcaa gaccaacaaa gagccgagca tgaagatcct cggtaagtat ctcaaaacca
     4261 acgaccgcga gctgctcgaa agttcctatg agatctatcg caaggacttc atttcggtgc
     4321 cgtacccgat cacccaaggt ttacaaccga cctatgagta cgtcgccgcg cagcgtccgg
     4381 agatttggca gcacaaaccg gaagcgttca tggacacgag cttcatcgcc gagctggaga
     4441 agagcggctt tataaagagt ctaactaagg cgcactgagc gaccgcgcgc ctttgcattg
     4501 cggataaccc gagcagcccc aaaactgtga tccggcaagc tcgcctttgc gcgcggtgcg
     4561 cacgaccatg agcttaccgc aagcaggaca gtttggcgca gcggcagttt gatccgacag
     4621 atcggatcga gccgtcggat ccgtccgatc tttctgctgc ctgcgccgct ccgccagtcg
     4681 tgcaacagca agctgctccg tgtaaccgcc ctcctgaaca aagccctttt ccagcgcggc
     4741 gatctgctgg tcgagcaagt agttcgcctg gtgaatcagg cagagcagag tgtttgcgac
     4801 gaccgccgga tctgcggcat cgagccagcg cgagtatttt ccccgatgat ccgtcggatc
     4861 ggaccgatcg gtcggatctc ttgataaccc caccgcgcgc acttcgcgag ccgccgggtc
     4921 atcttttgcc cactggcgct tgccgcgctg tctgagaaaa tcctcgtagt ccagcagcaa
     4981 ctcatcgaga ctcgcccgcg ccacattcac caagcgcagc tccgtctggc tcgacgtcgc
     5041 cgccgctcga ctaccctcgg caatgttttg ccggccgcta cgcgctgcct ggaccatctg
     5101 atcgtgggtg cgcgagcgct tgtcgataaa gcgatcgcaa aacgacaccg tgaaatcgta
     5161 gatcaccgtc gctacttgga agctgtgcag agcgcgatag ccgccgctcg gtcgcaagcg
     5221 gcgcggtcgg gtggatcggt ctgatccgac ggattctttg ttactcatag cgaatctact
     5281 tgatagcctc gcgctgtttc agttcctcga tcctcgccgc actcaacccg agtatctcgc
     5341 caaacacctg atcattattg acgccgatcc acggcgccgg ccgcgccgca tcggctttcg
     5401 atagtgatgg aatgccgggc aacccatggt gcgtgatctt gccccagggc tgcgcttcgg
     5461 tttcgacgat atgtccacgc gcgcgcagat gaatgtcatg aaacagatcc tcgccggagg
     5521 atggaatgcc ggccgccacg ccggcctctt gaagcaatcg aaagatttgt tttgccgtat
     5581 acttagcggt ccagtttgaa agcttttgat caatctcgag acgccgttct tggcggccca
     5641 ctttggtcgc gaatttgtca tcggc
//
LOCUS       DASQKS010000365         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2304613, whole genome shotgun sequence.
ACCESSION   DASQKS010000365 DASQKS010000000
VERSION     DASQKS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304613"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VE170_09510"
     CDS             <1..889
                     /locus_tag="VE170_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYJ15715.1"
                     /translation="RSGVAHFTANTEKDCLLMMRELVGFLPSNNQEDPPFRPTSDDPL
                     RRDKRLKDIIPDNPNRPYDMKELIGAVVDEGYFYEIQPEFARNILIGFAHIGGRSIGI
                     VANQPNHLAGCLDINASVKAARFIRFCDCFNIPIVTFVDVPGFLPGTEQEYNGIIRHG
                     AKLLYAYAEATVPKVTIVTRKAYGGGYIVMSSKHLRGDVNLVYPTGEIAVMGPEGAIN
                     IVFRGELEKSADPTKTRDELVNNYRETFANPFKAAESGYVDGIVYPEDTRPTLIRALE
                     MLKNKQDHNPPKKHGNIPL"
     gene            949..2481
                     /gene="accC"
                     /locus_tag="VE170_09515"
     CDS             949..2481
                     /gene="accC"
                     /locus_tag="VE170_09515"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HYJ15716.1"
                     /translation="MFKRILIANRGEIAVRIARACRELDIESIAVYSDADRESLHVKY
                     ADYAYAIGPAPSAQSYLVIDRIIDVCKKSGAEAVHPGYGFLAENAKFAQRCKDEGIVF
                     IGPSPEVIDQMGDKVKARQLMKAAGVPVVPGSDGILTSEEEVQQTMEMIGYPCMLKAV
                     AGGGGKGLRLVRSRREVSSAYRAVGSEAKSSFGDPRLYVEKFIVKPRHVEIQVLADSY
                     GRVIHLYDRECSIQRRHQKVIEESPAPGLEDRTRRRIGRIAIQGARSVGYVGAGTLEF
                     LLDSEQNFYFLEMNTRLQVEHAVTERVVGIDLVKAQIEIAAGGYLPWRQRHITQTGHA
                     IECRIYAEDPERDFMPCPGRIEGLRLPEGLGLRNDNGVYEGAEVPIYYDPMIAKLIVW
                     GENRVEAILRMRRALREYQVRGIKTNISFHQWILRHPRFMSGDVNTGFIEEEFKPKAN
                     EEVYPHKEIALASAAIAALHGEHERTIGLLAKGAAEPSKWRQQRAALRRPAAPGNRGW
                     RR"
     gene            2493..2996
                     /locus_tag="VE170_09520"
     CDS             2493..2996
                     /locus_tag="VE170_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYJ15717.1"
                     /translation="MAFVGKLGDQTFTVDIEETAPSVYRVSVDGNEFIVDGKKTGRTN
                     YSLIVDNRSFEIEVDNTDDDYRVLVDGRNYHVNLVDERRVRVGGAQGGLELQGRQKVV
                     VPMPGKILAVLVSDGDQVEKGQGLVIVEAMKMENEVHSPIAGEVKEIKVKPGDTVESG
                     ALLLIVE"
     gene            3067..3618
                     /locus_tag="VE170_09525"
     CDS             3067..3618
                     /locus_tag="VE170_09525"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HYJ15718.1"
                     /translation="MVPKKCFFTKGVGVHKEKLASFELALRQAGLAYCNLVLVSSIFP
                     PYCKRLSREEGAKLLRPGEVVFSVYDRESTNEPNRLVAASVGVAIPSDESQYGYLSEH
                     HSFGETDEKAGDYAEDLAASMLATTLGIEFNPDTAWDEREQLFKMSEKIVRTSNITQS
                     AIGNKDGLWTTVFAACAFINDDN"
BASE COUNT          840 a         1065 c         1112 g          805 t
ORIGIN      
        1 caggagcgga gtggcgcatt ttaccgccaa taccgaaaag gattgcttgc tgatgatgcg
       61 tgagctggtc ggttttttgc ccagcaacaa tcaggaggat ccgccgtttc gcccgacctc
      121 tgacgatccg ctgcggcgcg acaagcggct caaagacatt atccccgata atcccaaccg
      181 cccgtacgac atgaaagagt tgatcggcgc ggtggtggat gaaggctatt tctacgaaat
      241 ccaaccggaa ttcgcacgca atattcttat cggcttcgcc catatcggcg gccgttccat
      301 cggtattgtt gccaatcagc cgaatcattt ggccggctgt ctcgacatca atgcctcggt
      361 gaaggcggcg cgctttattc gtttctgtga ctgtttcaat atcccgatcg ttacttttgt
      421 cgacgtgccg ggttttttac ccggcaccga acaggaatat aacgggatca ttcgccacgg
      481 cgcgaagttg ctgtacgcct acgccgaagc gacggtgccg aaggtgacta tcgtgacgcg
      541 caaggcttac ggcggcggct acatcgtcat gtcgagcaaa catctgcgcg gcgatgtcaa
      601 cctggtctat cccaccggcg agatcgccgt catgggcccg gaaggggcga tcaacatcgt
      661 cttccgcggt gaattggaaa aatcggccga cccgaccaag acccgcgacg agctggtcaa
      721 caactatcgc gagacctttg ccaatccgtt caaagccgcc gagtcaggct acgtcgacgg
      781 catcgtttat cccgaggaca cgagaccgac tttgatccgc gccttggaaa tgctcaagaa
      841 caagcaagat cacaatccac ccaagaaaca tgggaatatt ccgctgtaga tacctcacga
      901 tgcgagcgcc agggcagggc gagcctggct tcgatatccc ttcgcaaaat gtttaagcga
      961 atcctcatcg ccaatcgcgg cgagatcgcc gtgcgcatcg cgcgggcttg ccgcgaactc
     1021 gatatcgaat ccatcgcggt ctattcggac gccgaccgcg agtcgctgca cgtcaaatac
     1081 gccgattacg cctatgcgat tggtccggcg ccgtccgcgc agagttattt ggtcatcgat
     1141 cggattatcg acgtctgcaa gaaaagcggc gccgaagcgg tgcatccggg ttacggcttc
     1201 ttggcggaga atgccaagtt cgcccaacgc tgcaaagacg aaggcatcgt cttcatcggc
     1261 ccttcgcccg aagtcatcga tcagatgggc gacaaagtta aagcccgcca gctcatgaaa
     1321 gccgccggcg taccggtggt gcccgggtcc gacggcattc ttacttccga agaggaagtg
     1381 cagcagacaa tggaaatgat cggttatcct tgcatgttga aagcggtggc cggtggcggt
     1441 ggcaaagggc tcaggctggt gcgctcgcgg cgcgaggtgt catcggctta tcgtgccgtc
     1501 ggttccgaag ccaagtcgtc cttcggcgat ccgcgcctgt acgtcgaaaa gttcatcgtc
     1561 aagccgcgtc atgtcgagat ccaggtgctc gccgacagct atggccgggt gattcatctc
     1621 tacgaccgcg agtgttcgat ccagcggcgc caccagaaag tcatcgaaga gtcgcccgcg
     1681 ccggggctgg aagaccgcac ccgccggcgc atcggccgga tcgccattca aggcgcccgc
     1741 tcggtcggtt acgtcggcgc cggaaccttg gaatttttgc tcgacagcga gcagaatttt
     1801 tattttttgg aaatgaacac tcgcttgcaa gtcgagcatg cggtcaccga gcgggtggtc
     1861 ggcatcgatt tggtcaaagc gcagatcgaa atcgccgccg gcggttattt gccgtggcgt
     1921 caacgccata ttacgcagac cggccatgcc attgaatgcc ggatttatgc cgaagatccc
     1981 gagcgtgact tcatgccgtg ccccggccgg atcgaaggct tgcgactgcc cgaagggctc
     2041 ggcttgagaa acgataacgg cgtctacgaa ggcgccgagg tgcccatcta ctacgatccg
     2101 atgatcgcca agttgattgt atggggcgag aatcgcgtcg aggcgattct gcgtatgcgc
     2161 cgggcattac gcgagtacca ggtgcgcggc atcaagacca atatttcatt tcatcaatgg
     2221 atcttgcgcc atccccgttt catgtccggc gacgtcaata ccggatttat cgaggaagaa
     2281 tttaaaccca aggccaacga agaagtctat ccgcacaagg agatcgcgct cgcctcggca
     2341 gctatcgcgg cgctgcacgg tgaacatgaa cgaaccatcg gcctgcttgc caaaggcgca
     2401 gccgagccgt ccaaatggcg ccagcagcgc gcggcattgc gccggccggc cgcgcccggc
     2461 aatcgcggct ggcgccgtta aaagatatcc ccatggcgtt tgtcggaaaa cttggcgatc
     2521 aaactttcac cgtggacatc gaggaaacgg ctccctcggt ttaccgggtt tcggtggacg
     2581 gtaatgaatt catcgtcgat ggcaagaaga ccggccggac caattattcg ctgatcgtcg
     2641 acaatcgttc cttcgagatc gaagtcgaca acaccgacga tgactaccgg gtgctggtcg
     2701 atgggcgcaa ttaccacgtc aacctggtcg atgagcggcg tgtgcgcgtc ggcggggctc
     2761 agggcggcct tgagctccag ggacgtcaga aggttgtcgt gccgatgccg gggaaaattc
     2821 tcgcggtcct agtttccgac ggcgaccagg ttgaaaaggg ccaagggctg gtgatcgtcg
     2881 aagcgatgaa gatggaaaac gaagtgcata gcccaatcgc cggcgaagtc aaagagatca
     2941 aagtaaagcc gggcgacacc gtcgagagcg gcgccttgtt gttgatcgtc gagtagacac
     3001 aaattaatca agatttctaa agtcgattgt tgtcagccta tttctttcat gttatagcag
     3061 cgcaccatgg taccgaaaaa atgttttttt accaaaggcg ttggtgtcca taaagaaaag
     3121 ttggcgtcct tcgagctggc attgcggcaa gcgggtctgg cttattgcaa cttagtgctg
     3181 gtgtcgagca tcttcccgcc ctactgcaag cgactttcca gggaagaggg cgccaagcta
     3241 ctccgtccag gggaggttgt gttcagtgtc tacgaccggg agagcaccaa tgagccgaac
     3301 cgtttggtgg cggcctcggt gggtgtggcg attccatcgg atgagagcca atacggttac
     3361 ctgtcggagc accattcctt cggcgagaca gacgaaaagg ccggcgatta cgccgaggat
     3421 ctcgccgcca gcatgctagc gaccacgctc ggcatcgagt tcaatcccga taccgcctgg
     3481 gacgagcgcg agcagctgtt caaaatgtcc gagaaaatcg ttcgtacttc caacatcacc
     3541 cagtccgcca tcggcaacaa agacggccta tggaccacgg tgtttgccgc ctgcgcgttt
     3601 atcaacgacg ataactagat ttttagcttc acagtgatca ggggtgttgc cttcgggtgc
     3661 gcctcttttt tatttgggat tcaggctttt gttgggcagt gtgtcggctt ccgctcgttt
     3721 gtttacagca aaattagcct tcaaccgcgg ccaggcaagc tccctacgcg ctgggttcgg
     3781 aggattgatt tagagggaaa atctttcgcc gcgacaacga ac
//
LOCUS       DASQKS010000366        11457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3967323, whole genome shotgun sequence.
ACCESSION   DASQKS010000366 DASQKS010000000
VERSION     DASQKS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3967323"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VE170_09530"
     CDS             complement(<1..814)
                     /locus_tag="VE170_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15719.1"
                     /translation="MTQQLKVIDGDGHIFEDSDGIARHFPYSATGARLRSGVFPLNSH
                     IQYSLTRTPPGAFATNSEGRFVNPGPEGWIKFIEEAGIDHAVLFPTAGQRIGRIVDRD
                     YACGAARAYNDWLAETYLRRDDRFKGVALLPMHDADAAVAELQHAQKDLGMAAVLFPA
                     TGVHLNLGAKPYWPVYAEAARLGIPVVVHGGGHWDLGMETMNVSTGANAIGHPMSLAI
                     ALAEMVLNNLFERFSGLRVAYLEGGPLWFLMALERLSRSFEAGTPVNPRGELL"
     gene            complement(1304..2824)
                     /locus_tag="VE170_09535"
     CDS             complement(1304..2824)
                     /locus_tag="VE170_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ15720.1"
                     /translation="MPTDKTLIKNWRGKNTEPESAGPQETLAAPFAPHILVVDDDAVI
                     RQQLEQLFTHEGYRVKVESGAEQALQLLQNEDVDLVVTDIRLPGMDGIELTKRIVERW
                     SDVPVIVMTGYADIDNAVEVLKNGASDYIVKPFSMAAIQESARVVLGKTGFFAEIRQL
                     RRELKDNYVFGRMLSRSPEMHRVFETIRTVAPTDSTVVLEGETGTGKELMARTIHHQS
                     FRRNGPFITINCGGFPETLLESELFGYERGAFTGAEQTRPGKIELAHEGTLFLDEIEN
                     MPLAMQVKLLLVLNDQKVQRLGSSRWTKVNMRVVAASNIPLKELLDQGKVRSDFYYRI
                     RVISIGLLPLRRRLDDIPLLIKDFLQHHPVSVRKKITGVLPDAMEQIRRYPWPGNIRE
                     LHNVLEKAVIMTKSRVLNAADLDLEVLHRNLPGDGYKRSVSTELSLNEWIREREKEYL
                     TDKLKACRGRIDLTAKSCGVDVRTIHRKLQIYGLDKKAFGKPNPKNNVTPEGDRDH"
     gene            complement(4391..5287)
                     /locus_tag="VE170_09540"
     CDS             complement(4391..5287)
                     /locus_tag="VE170_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15721.1"
                     /translation="MATGGETIYEWDKNPRMPAPPPPAGSCDCQFHIYGDPIQFPPRT
                     NAPYPPIESATFTEAQRMHKAMGFARGVIVHSAIYGSDHRLLLHALESLNDRDRYRGI
                     GIVDDRVSDMELARLHAAGVRGARFNFVRFFAMEQREAEVRRSMARIKELGWHARLHV
                     NGEDLLENSTLLRSLKDIPMVIDHFGHVGFNGGMDRPVIRWVLDSLKLDNWWVMVSNG
                     NRDSHMDTKWDDALPYGKAFIEAAPDRIVWGTDWPHPQWKKPMMNDAEEVELLYRYVD
                     HDVSLLKKILVDNPARLHGFDI"
     gene            5566..6765
                     /locus_tag="VE170_09545"
     CDS             5566..6765
                     /locus_tag="VE170_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15722.1"
                     /translation="MAQQTSNSVEWQEPDNGQNAGIGSWNRPNSPYDNFMESEGVPIY
                     RGIGTQRVQELPFKPWRRLGGKGTFIQLYGTEGLWGCYVVEIPGADGLKAERHMYEEV
                     YLVVEGRGTTEVWVDGSAHKHQFEWQTGSLFSIPMNAWHRVVNARSTPAVLLAATTAP
                     NMVNLVRNSDFIFNCPYVFKDRYNDAEDFFKPNVEVVPDPLRGLAMSRTNLIPDVFNS
                     ELQLDNRRSPGYRRMEPRMTENVFYQFIGEHENGRYSKAHAHASAAVLVCIKGKGYTY
                     TWPVKYGLTPWKDGHREQIIRQDYDHVGMVSAAPMSGDWFHQHFGVSKEPLRLLGWYG
                     PNNHRAQQPGRPGEKAIDEGAIDVRDGGTAIPYDEEDPFIRAEYEETLKQEGVASRMT
                     DDLFRRK"
     gene            6970..7965
                     /locus_tag="VE170_09550"
     CDS             6970..7965
                     /locus_tag="VE170_09550"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ15723.1"
                     /translation="MSRAKCWLIFLTVVVAFAAPVRGQNLPKVRAAYTSIGIQFDPVY
                     IMKELDLPRKHGLDVEVLFVPVSSRALQAALAGEIQFLTSGGVANINANATGADFVGL
                     TATLNTFAFKILGSPDIKKPENLRGKKVAISRLGGASDFSVRYALTHWGLVPDKDVAL
                     LQVGGEPESVLALQNKAVDAAILSEPFSTVATRAGSVLVADLSQLGVPYTMHGFGVRK
                     SFIRANRDVVVRFMKAYLEGIYLFKTNKELALNVLKKYTRLDDLSLVQVSYDEMSQRL
                     IRRVPYPDREGIQTIIDQLAKTRPQMKTLNAGDFIDPSILKEIEDSGFMKKLYGN"
     gene            8786..9811
                     /locus_tag="VE170_09555"
     CDS             8786..9811
                     /locus_tag="VE170_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15724.1"
                     /translation="MYIPAFRPTVSFRLTTLLLVGCLLGGEAFAQSSPVKPEKSQLEV
                     AIAAWGATSIPTAVALDGGYFAKQGLAVNISVVAASTSVQALISGRVDIYQGGATAIG
                     GNLGGADIIYVGASVDKSSLMLLSQKSLTSFEQFRGKTISTTSPGAFGEIVVRMTARK
                     HGMEVGKDVKLIYHRTPGEALSTFLAGNADGLVIGPPHTDLARQKGATVVVDYYKEGV
                     KIIGPGTAVMREFAQKNPNTIKAYFMGHLDGLKRAVDDEEYAKKLDGKYSKLTDAKLL
                     AENYRQGLRVWNKDMSVDPAAIRVVLEDSPDGKGKGADPKRFYDNSLIQAVNRDYASK
                     LFPGEVK"
     gene            complement(10012..>11457)
                     /locus_tag="VE170_09560"
     CDS             complement(10012..>11457)
                     /locus_tag="VE170_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYJ15725.1"
                     /translation="WISPIYPSPMADFGYDVSDYTDIDAIFGSLADFDALLAEVHGRG
                     MKLLLDYVPNHTSNHHPWFIESKSSRSSAKRDWYIWRDAAPDGGPPNNWRSNFGGAAW
                     TWDEATGQYFYHAFLKEQPDLNWRHIELRQAMLDVLRFWLSRGVDGFRVDVMHHLAKD
                     TRYRDNPTNPNFRPGMSPYRELLTTYTADLPEVQEIVAMMRAVIDEYDDRMLVGEIYL
                     PLERLMAYYGASGKGAHLPFNFQLIRLSWNAMEVAAAVERYEAMLPSYAWPNWVLGNH
                     DKPRIATRVGAAQARVAAMLLLTLRGTPTLYYGDELGMHDVSIAPDQVRDPFEKNVPG
                     LGLGRDPVRTPMQWTGGDCAGFTTGSPWLPLADDYKTVNAAALREQPTSILTLYHRLI
                     ALRRAEPALSVGAFASLPVVDGVMAYVRKSSERRLLIILNFVPQPRNFDLRTLDAQAV
                     LLLSTHLDRSQEQLGDEIQLRSDEGVIVELR"
BASE COUNT         2621 a         3001 c         3172 g         2663 t
ORIGIN      
        1 gcagtaattc cccgcgcgga ttgaccggcg taccggcttc gaacgatcgc gacaggcgtt
       61 cgagagccat gagaaaccac agtggaccgc cttccaggta agcgacgcgt agaccggaga
      121 agcgttcgaa caaattgttg agtaccatct ccgccaaagc gatcgccagc gacatcgggt
      181 ggcctatggc gttggcgccg gtggacacgt tcatggtttc cattcctaag tcccagtggc
      241 cgccgccatg gacgacaaca ggaatgccca agcgcgccgc ttcggcgtac accggccagt
      301 agggcttggc gccgagattc aaatgcactc cagtggcagg gaacagcacc gcggccatgc
      361 ccaagtcttt ttgcgcatgt tgcaattccg ccacagccgc atcggcatcg tgcataggta
      421 acagcgcgac tcctttaaaa cggtcgtcgc gtcgcagata ggtttccgcc agccagtcgt
      481 tgtaagcgcg cgcggcgccg caagcgtagt cgcggtcgac gatccggccg attcgttggc
      541 ccgcggtggg aaagagcacc gcgtgatcga tccccgcctc ctcgatgaac ttgatccagc
      601 cctcgggacc cgggttgaca aaccgaccct ctgagttggt cgcgaatgcc cctggcggcg
      661 tgcgtgtcag ggaatactgg atgtggctat tgagcggaaa caccccgctg cgcagccgtg
      721 cgccggtagc gctgtaggga aagtgccggg cgatcccgtc gctgtcttcg aaaatatgac
      781 catcgccgtc gatgactttc aattgctgtg tcattgattt ctcctgtacc aacgtcttgc
      841 caagcgtgat attgccgagg tttatttgag acgtgcgcga ttgcgtcggg ccatggctga
      901 gtccggcgcc caaccattgt cacaacacct tcctcatctg cctgatagca ccgcgcgaca
      961 aaaattgtca acctgcgtcg acgcgctcgc cggcaccggt caacgcaggg cactcttaga
     1021 actcgatcga agcaacgacg aaacaaaggc cagttgatga gacgaagcga acccgggtga
     1081 gttagacgcg agcggcaatg aggaaactag cagaggtttt tatttagaca gtggaataag
     1141 ctgcttgtgg ctatgccgtg atgtgagggc catcaagaag cggttaaagc cactgcagac
     1201 attctaaaag tttcccagtc gttgtcggcg tgagcgacgg tatcaacaac tgataaccag
     1261 ggatttgcct gacgaatgcc ggttaagata gtgacttata tacctaatgg tctctgtctc
     1321 cttcaggggt aacgttgttc ttgggatttg gctttccgaa ggctttttta tccaggccgt
     1381 agatctgcaa cttgcggtga atcgtcctca cgtcaacacc gcaactcttg gcggtgaggt
     1441 ctattcggcc tcgacatgct ttgagcttat cggtcagata ctccttttct cgttcgcgaa
     1501 tccactcgtt gagagatagc tcggttgaga cacttcgctt gtaaccatcc ccagggagat
     1561 tgcgatgtaa tacttccaaa tccaggtccg ctgcgtttag aactcgggat ttggtcataa
     1621 tcaccgcttt ctcaagaacg ttgtggagtt ctcgaatatt gcccggccag ggatatcttc
     1681 gaatttgttc catggcgtcc ggcaatactc cggttatctt cttcctgacg gaaaccggat
     1741 gatgctgcaa aaagtccttg atcaacaacg ggatatcgtc caatctccta cgcaagggaa
     1801 gcaggccgat cgagataacc cggatgcggt aatagaagtc gcttcgcact ttgccctgat
     1861 ccagcagttc ttttaatgga atgttgcttg ccgctactac gcgcatattg accttagtcc
     1921 aacggctcga gccgaggcgc tgtacttttt gatcattaag aaccagcaat agttttacct
     1981 gcatggccag cggcatgttc tcgatctcgt ccaagaacag cgtgccttca tgggcgagct
     2041 caatctttcc tggtcttgtt tgctctgcgc cggtaaaggc cccacgttcg tatccgaata
     2101 gttcgctttc aagaagggtt tcgggaaacc cgccgcaatt aatagtgata aagggaccat
     2161 tcctgcgaaa actctgatga tggatcgtcc ttgccatcaa ttccttgccg gttccggttt
     2221 ccccctcaag caccacggtg ctgtcagtcg gggccaccgt acggatggtt tcgaaaacgc
     2281 gatgcatttc aggagacctg cttagcattc tcccaaacac atagttatct ttaagttccc
     2341 gtcgcagttg gcggatttcc gcgaagaagc cggttttccc tagcaccacc ctggcagatt
     2401 cctgaatggc tgccatgctg aagggtttaa cgatgtaatc actggcgcca ttcttaagga
     2461 cttccacagc gttgtcgatg tccgcgtagc ccgtcatgac gataactgga acgtcactcc
     2521 agcgttcgac aattcgtttg gttaattcga tcccatccat tccaggtaac cgaatgtcgg
     2581 taaccacgag atcgacatct tcgttttgca ggagttgcag cgcttgctcg gcgcccgatt
     2641 cgactttaac cctgtaaccc tcatgcgtga acaactgctc taactgttgg cggatgactg
     2701 catcgtcgtc gacaacgagg atgtggggcg cgaacggcgc ggctagggtt tcttgaggtc
     2761 ctgcggattc cggctcggta tttttgccgc gccaattttt tattaaggtt ttatctgtcg
     2821 gcatatcgct agacaaccgg ccatctagat gaaccacggc gcagcagccg cgggtatcac
     2881 aaaagccgga atttctcctg gtgtattggt tttagtaact actttacccg acaattaggc
     2941 ccatattcgt cagcgtcgta agctccgagt cctttcgtcc cccaaaaaat tcttatttta
     3001 tcttacgcgg attttcgccg ccttaactgc ctaccgtctt catcaggatt gttaacatcg
     3061 gccatcgcca tggcgctcga cgaacctatt gaaggtcgat ttgacgggct aggctttttt
     3121 ttacgcacgt cgtgcaacgc catgtcgaga atggcccgcc ggagtttggc atccgaggcc
     3181 ccgtttcgcc ggctgaatgc agtcccggag atcttgcaac gaatccccca tggaacgcat
     3241 ctcgcttagc agcctgctaa aatcggcgcg cttatatacg ccggcgtccg caagttctaa
     3301 tcccatgctt accttgtgga gacgatttgt taggtcgtga attaaatgtt caatgatcgt
     3361 tcccgtaacg gtgtcattgg tctcggttga gaccgcggcc ttcaggtcag agatttccgt
     3421 gaatgtactg atgacatccc agggactagg gtcttctacc ttggcaagaa gcgaaatatc
     3481 tctaatccag atgggttgcg ttgcgccttt aggaaggaat cgataatcac aggaagccga
     3541 cggtacgttc agagcgagct ccttttgaga ttggcaaaac atctgttgat cagcgggatg
     3601 aatgcactcg gtccaaaagg aaggacgatt ggagagatct ttttcagaat atcggattag
     3661 gcgttttgca ctttcgttta acaagtaaaa ggatcggtcc cgactgcgca atgccgccgg
     3721 gcagggaatc ctctctaaaa aatctcgcgc gttgtcgaat tgtattgatc cacacggagt
     3781 tgtttttcta tcgaagctca tacaagttct cgtgtcattt agtttgaaaa ttactgacct
     3841 ttcgccgcaa tttgtggaag acggccaacc ataaagaaac agatggggta atgtcaacaa
     3901 cctttgtgtt aaaatctgtg acaaatagct gttgtgtaaa gtttttttac aatgccgtca
     3961 gactagttta aattgctatg ccaaggggtg tccgcccaag ctacattcgt gcctattttc
     4021 gttgccgttt tgcggcggga attcataggc aatgctggag aacaattgcg cctactatgc
     4081 accgtcatcg ggacaatttg agtggtcgag cctccgagtt tcaaatcttc agagatcagt
     4141 tcagaaattc tacacggttc gaagtccgat gaactgagct accgacctgg taaagcgcct
     4201 gcgcatatac tacggggtcg tgactgcctg acctgatata gcacggccaa actcgctagc
     4261 ctcgggatcg aatcgctggg tcacaatgag aatggcgttc tatttcgatt gaagggaaaa
     4321 tcatcgtccg tccaaatctt cgtggctgtg gattggctct tcgtctgtca ctcggaggca
     4381 ttggcgtgcc ctaaatgtca aatccgtgca gccgcgccgg attgtctacc aaaatttttt
     4441 tcaatagcga cacgtcgtgg tcgacgtagc ggtagagcaa ctcaacttcc tcggcgtcgt
     4501 tcatcatggg tttcttccat tgtggatgcg gccagtccgt gccccagacg atacggtcgg
     4561 gcgctgcttc gataaacgcc ttgccatagg gtaaggcgtc gtcccacttg gtgtccatgt
     4621 gggaatcgcg gttgccgttg gaaaccatca cccaccaatt atccaactta agtgaatcga
     4681 gcacccaacg gatcaccggc cggtccatgc cgccgttaaa gccgacatgg ccgaaatggt
     4741 cgatcaccat tgggatgtct ttgagcgagc ggagcaacgt agaattctca agtaaatcct
     4801 cgccgttgac atggagtcgg gcgtgccagc cgagttcctt gatgcgcgcc attgagcgcc
     4861 ggacctcggc ctcgcgctgc tccatggcga agaagcggac gaaattaaag cgcgcgccgc
     4921 gcacaccggc cgcatgcaag cgcgccagtt ccatgtcgga aactcgatcg tcgacaatgc
     4981 cgataccgcg gtaacggtcg cggtcgttca agctttcaag agcgtgcagc aaaagacggt
     5041 gatcgctgcc atagatcgcg ctatgaacga tgacgcctcg agcgaagccc atggccttgt
     5101 gcatacgttg cgcttcagtg aacgtcgcgc tttcaatcgg cgggtagggc gcattggtcc
     5161 gcggcggaaa ctgtatcgga tcgccgtaga tgtgaaactg gcagtcgcag cttcccgcgg
     5221 gcggcggcgg cgccggcatg cgcgggtttt tatcccattc gtagatcgtc tctccgcctg
     5281 tagccattgt cgtgctcccg caactcttac gatattttca gaatccgcct tcaacttttg
     5341 aaacttgaac cctgaacaaa ttgatctggt caacagggtt cccgttccac gatcgtgcaa
     5401 cgctacatct cttaactgga acgagtcaag aaaagtgact aatttgcctt aattcctctt
     5461 aggttatggc atatagtaaa aaaataccgg aagccgcggt gaagcacaat gttgtcattt
     5521 atcgcaggca caaatttttg catttcatca cacaaggaga ttttcgtggc gcagcaaaca
     5581 tcgaattccg tagagtggca agagcctgac aatggccaaa acgcgggtat cggcagttgg
     5641 aatcgtccaa actctcctta tgacaacttc atggagagtg aaggtgtgcc aatctaccgg
     5701 ggcatcggca cgcagcgggt gcaagagctt cccttcaagc cgtggaggcg gctcggcggc
     5761 aaaggtacat tcattcagct ttatggcact gaagggctgt ggggttgtta cgtcgtggag
     5821 atccccgggg ccgatggcct caaggccgag cgccacatgt acgaagaagt ttatttagtc
     5881 gtcgagggcc gcggcaccac cgaagtttgg gtcgacggtt cggcgcataa gcatcagttc
     5941 gaatggcaga ccggctcact gttttcgatt ccgatgaacg cttggcaccg tgtggtcaat
     6001 gcccggtcga ctccggcggt attactggcg gcgaccacgg cgccgaatat ggtcaacttg
     6061 gtgcgcaaca gcgatttcat tttcaactgt ccctacgttt ttaaagatcg ctacaacgac
     6121 gccgaggatt tcttcaagcc taacgtcgag gtcgtgcccg atccactacg cggcttggcc
     6181 atgagccgga ccaatctcat cccggatgtc ttcaatagcg aactacagtt ggacaaccgc
     6241 cgctccccag gttaccggcg catggaaccg cgcatgaccg aaaatgtttt ttatcaattc
     6301 atcggtgaac atgagaacgg ccgctattcc aaagcccatg cccacgcgtc ggcggcggtg
     6361 ttggtctgca tcaaagggaa aggttacacc tacacctggc ccgtgaaata tggtctgacg
     6421 ccgtggaaag atggtcatcg cgagcagatt atccgtcaag attacgatca tgtcggcatg
     6481 gtcagcgccg cgccgatgag tggtgactgg ttccatcagc atttcggggt aagcaaagaa
     6541 cctttgcgcc tgttgggatg gtatggcccg aataaccacc gtgcccagca accggggcgg
     6601 cccggggaaa aggcgatcga cgagggcgcc atcgatgtcc gtgacggtgg cacagcgatt
     6661 ccctacgacg aggaggatcc atttatccgt gcggagtacg aagagacgtt gaaacaagaa
     6721 ggggtggctt cgcggatgac ggatgacttg tttcggcgga agtagattga cgaagatcaa
     6781 acagaagcgt gaagtgcaca cgccatgaca tgacgaatgc aaagtcggaa aacacgggtg
     6841 atgggcttca taaggctcca ggcgaagtcc ccagggaagg gccaatacag aagtcagcta
     6901 ggctatgtgt tcaacacatc cctcggcggg aggccaagca gagttcgatg acggataacc
     6961 ggaggaagca tgtcgagagc taaatgttgg ttaatttttt taacagttgt tgtcgcgttc
     7021 gctgcgccgg tgcgaggaca aaacctgccc aaagtccgcg ccgcctatac ttccataggt
     7081 atccaattcg atccggttta catcatgaag gagctcgacc tgccgcgtaa gcatggcttg
     7141 gacgtcgagg tgctatttgt tccggtgagc tcccgagcgc tgcaggcggc gctagcgggt
     7201 gagatccagt tcctcaccag cggcggtgtg gccaatatca acgcgaatgc gactggcgcc
     7261 gatttcgttg ggttgaccgc aacgctcaat acttttgctt tcaaaatcct tggcagccca
     7321 gacattaaga agccggaaaa tttaaggggc aagaaggtgg cgatttcccg cctcggcggc
     7381 gccagtgatt tttccgtccg ctatgcactc acccactggg gcctggtacc ggacaaagac
     7441 gttgcccttc tccaggtggg cggcgagccg gaatcggttt tagcattgca aaacaaggcg
     7501 gtggacgcgg cgattttgtc ggaacccttt tcgacagttg ccacacgagc gggctcggtt
     7561 ttagttgctg atctgagcca gctcggcgtg ccttacacga tgcatggctt tggcgtgcgc
     7621 aaaagtttta ttcgcgctaa tcgtgatgtc gtcgtacgat tcatgaaagc ctatctcgaa
     7681 ggcatttact tgtttaaaac taacaaggaa cttgcattga atgtcctgaa aaaatacacg
     7741 cggctcgacg atttgtcgct tgtgcaagta tcctatgacg agatgtctca gcgactgatt
     7801 cgcagagttc cgtacccgga ccgcgaaggc atccagacca tcatcgatca actcgccaag
     7861 acccggccgc aaatgaaaac tcttaacgcc ggtgacttca tcgacccatc gatcctgaag
     7921 gagatcgagg atagcggctt catgaaaaag ttatatggca actaattgtc acggtttgcg
     7981 aacggcaatc aagtgtgatc actcgctttg ggtggttcac aaagatttaa ggattgctta
     8041 aggcagtgcg gattcggcag acgtcctgac gcctcgacgc cgcgaatcgg cccatgcact
     8101 tggtgactca gagtaaatta acggaaggga ctacgccaaa gtagaaaaag ggccgggttt
     8161 ctccagacca cgtcttttta cgaggtaacg cgttacattt cgtctccagg cgccccgtgc
     8221 ggagcgggac caacaagaaa gcgcggattt aatgttactt ctcaaatgga gtgggggcac
     8281 gctgagcgtt aattcttttt tttccgcttg ggttgcttcc atacagatga ctccttccca
     8341 atggttgggt gagcaaccta cgtaccaagt ccgacaaacc cataatttca atatgttaga
     8401 gggaaatagg tcctacaggc ggattcttta gaagacattt ttgttatgtt aaaggatctt
     8461 atcttacaaa ctcggtcctt attgcgcttc attgggccaa actgatcttt agcgccatcc
     8521 tggggacgat ctgtgcgctg ctgtgagcgg tagctaaaaa tccgcacttc gacgatcggt
     8581 ccttggaaac attcctcata ggaactgatg tctgtcgctg ctccgccgat cgtaaaagcg
     8641 gtgccctcac agtcaaggcg ggtctgggat agtgatcgcc tcgaaattgt cattgcggtg
     8701 agttgcagcg ccatgctaaa ggctccgtag gtgatgggta acgtgaataa gttatcgatg
     8761 agatgctatg aggggactaa gtaaaatgta cattcccgcc ttccgtccaa ccgtgagttt
     8821 tcgtttgacc acattgctgc tggtcggttg cttgctcgga ggcgaagcct ttgcgcaaag
     8881 ttcgcccgtc aagccggaaa aaagtcaatt agaggtcgcg atcgcggcat ggggagcgac
     8941 atcgattccc accgcagtgg cgctagatgg cggttacttt gccaagcaag gtctggcggt
     9001 gaatatatct gtagtagcgg cgagcacatc ggtgcaggca ttgatctcgg gtagggtcga
     9061 tatttatcaa ggcggtgcca cggcgattgg cggcaatctc ggcggggcgg atattattta
     9121 cgtgggcgct tcggtcgata aaagttctct gatgttgctc agccaaaaaa gcttgacgtc
     9181 gttcgagcaa ttccgcggca agacgatctc gacaacctct cccggcgcgt tcggcgaaat
     9241 cgtcgtgcgc atgactgcgc gcaaacacgg catggaagtg ggcaaagatg taaagctgat
     9301 ctatcaccgg acgcctgggg aagctctgtc gacttttctg gccggcaatg ccgacggttt
     9361 agtgatcggc ccgccgcata ccgacctggc ccggcagaaa ggcgccaccg tggtcgtcga
     9421 ctattacaaa gaaggcgtga aaatcatcgg tccggggact gcggtgatga gagagtttgc
     9481 gcagaaaaat ccgaatacca tcaaagctta tttcatggga catctggacg ggctgaagcg
     9541 cgcggtcgac gacgaggaat atgcaaagaa gcttgacggc aagtattcca agcttaccga
     9601 tgccaagtta ctcgcggaaa attaccggca aggcttgcgg gtctggaaca aagacatgag
     9661 cgtcgatccg gcggcgatcc gcgtggtgct cgaagattcg ccggacggaa aaggcaaagg
     9721 cgccgatccg aagcggtttt atgacaactc attaatacag gccgtcaatc gtgattacgc
     9781 ttccaagctt tttcctgggg aagtgaaata gtgggcgcga tgcaagatgg gcatgaaagc
     9841 gcgcttagct tggccaccta caacggtccc gttcaaggcc taaatgattt atcgtcgagc
     9901 agcgcgcagc aagatagcca gtatcggcgc ttcaaggggc cggaaatctg gcaaaattac
     9961 cgacttttgt tgcgcctccc gaagtcgctc taatattcga tttgccgcgt ttcagcgaag
    10021 ctcgacgatg acaccttcgt cgctgcgcaa ttggatttcg tcgcccagtt gctcctggct
    10081 ccggtcgaga tgagttgaaa gcagtaacac ggcctgtgcg tcgagggtgc gcaggtcgaa
    10141 gttacgtggc tgcggaacga agttgaggat gatcaatagg cggcgctcac tcgacttgcg
    10201 cacatacgcc atcacaccat cgaccaccgg gagcgatgcg aaagcgccca cggacaacgc
    10261 tggctcggcc cggcgcagcg cgatgaggcg atgataaagc gtgagaatcg atgtgggttg
    10321 ctcgcgcaac gccgccgcat tgacggtttt gtagtcatcg gcgagcggca gccagggcga
    10381 gcccgtcgta aagccggcgc aatcgccgcc ggtccattgc atcggagtgc gcaccggatc
    10441 gcgaccgagt cccaagccag gaacgttttt ctcgaaagga tcgcgcacct gatccggtgc
    10501 aatagacaca tcgtgcatac cgagttcatc gccgtaatag agcgttggcg tgccgcgcaa
    10561 agtcagcagc agcattgccg ccacgcgcgc ttgtgcggcg cctacccggg tggcgatgcg
    10621 cggcttgtcg tgattgccga gcacccagtt gggccaagcg tacgatggca gcatcgcttc
    10681 gtagcgctcg accgcggcgg cgacctccat tgcgttccag gacaagcgaa tcagttgaaa
    10741 attgaatggc aggtgcgctc ccttgccgct tgcaccgtaa taggccatca gccgttccag
    10801 tggaagatag atctcgccga ctaacatgcg atcgtcgtat tcgtcgatca ccgcgcgcat
    10861 catggcgacg atttcttgca cttccgggag atccgccgtg tacgtcgtaa gcagctcgcg
    10921 atacggggac atgcctggac ggaaatttgg gttggtcgga ttgtcgcgat accgtgtgtc
    10981 tttggcgaga tggtgcatga cgtcgacacg aaagccatcg acgccgcggc tgagccaaaa
    11041 gcgcagcacg tcgagcatcg cttgccgcaa ttcgatatgg cgccagttaa gatcaggctg
    11101 ttctttgaga aaagcatgat aaaaatattg tccggttgct tcgtcccatg tccaggccgc
    11161 accaccgaaa ttggagcgcc agttgttcgg cggtccgcca tcaggtgcgg catcgcgcca
    11221 gatgtaccag tcgcgttttg cgctggaacg cgacgatttc gattcgatga accagggatg
    11281 gtgattcgaa gtatgattgg gaacgtagtc caacaacagc ttcatgccgc ggccatgcac
    11341 ctcggccagc aacgcatcga agtctgccag tgagccgaaa atagcatcga tatcggtgta
    11401 gtcgctcacg tcgtaaccga aatcggccat gggcgaggga tagatcggcg aaatcca
//
LOCUS       DASQKS010000367         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5640874, whole genome shotgun sequence.
ACCESSION   DASQKS010000367 DASQKS010000000
VERSION     DASQKS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5640874"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VE170_09565"
     CDS             <1..654
                     /locus_tag="VE170_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ15726.1"
                     /translation="ESGINGSGREILDRLVNNGQELSHLMDNLMNYSRMEADSGAAQF
                     EVVKLKEIFGGLELMTQRLIRQRQIQFAIVMEPAIETIESDGAKLQQILLELLTNALK
                     FTGKGKIELRSRRLEESAGEFLEIAVTDTGIGIRREDQQVIFEDFRQLDGSSTRQYGG
                     TGVGLGLCKKLAAALGGEIRLHSEVGVGSVFSLRLPLKSLGSHPGSPALAPGTGLLQ"
     gene            711..1424
                     /locus_tag="VE170_09570"
     CDS             711..1424
                     /locus_tag="VE170_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ15727.1"
                     /translation="MPNLRQLVIAAEDPERLAKFYQEVFDLEKIDDNQGALFLSDGTF
                     NLGLLPEADAEKCGFRHLGFDTARVESIRIKLAHVGLAQSEVSEVNSPATIDHELKDP
                     DGNTVGVSRRAFDVAYTRRPVPIRHIALYTPNPQKMAEFYCKVLDMKEVERTDRSSIF
                     VSDGYFNLALLYQRKEEPMGLNHFGFHVQSNEEMQTRAERAGVRRGAKRPDRIPFAEY
                     RVHDPEGNGIDISQKGWRV"
     gene            1511..2158
                     /locus_tag="VE170_09575"
     CDS             1511..2158
                     /locus_tag="VE170_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYJ15728.1"
                     /translation="MNGDEFLQEILAPVHEFCRMLDGRPPAYSYVPLRTDEERIRVMQ
                     SRLFNELRAADLFGTWLKTTPELDVKATMAESAHEEMVHAQLLSKRIRDRGAEPFDYR
                     PLPVQIAMFNAMEGLNDTCERIAGLSLAGETVANYLIQMCLKAPDVPEWIKQPYRKIT
                     EDEEGHGSVPQIFLKRYATTEERQDRVRRAVAMRMVLFREYLASLDRWALGQNRW"
     gene            2251..3180
                     /locus_tag="VE170_09580"
     CDS             2251..3180
                     /locus_tag="VE170_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ15729.1"
                     /translation="MKILFFDDFKLGVLKGDTVVDVSHVVQDIPHTGPHNLISGLIES
                     FNDYRSAIEKAVAAGSGVALKSVRIRPPLPKPVNIDCMAVNYMEDGTRKEPAPINAFH
                     KSPNSVIGDGDTMILPDVPAPIFEGEAELALVIGRRADNVTADQAMEYIFGYMNFIDG
                     SARGLPPAGNTFYQMKSRATFAPTGPFIVTADEITDPHNLQVRLWNNGVLMQSYHTSD
                     MAHKIPRCIEWVSSIHPLEAGDILALGTNHRGLNPFQDGDAIEIETEPLGRLHVKVRD
                     PLKRTWARETRLQRQEKGLEGFTPQITGKYAKA"
BASE COUNT          731 a          901 c          965 g          706 t
ORIGIN      
        1 gaaagcggta tcaacggcag cggccgggag atactcgacc gcctggtaaa caacggccaa
       61 gagctgtccc atctaatgga caacttgatg aattattcgc gcatggaagc ggatagcggc
      121 gcggcccagt tcgaagtcgt caagctaaaa gagattttcg gcggtctgga attgatgacc
      181 cagcgcttga tccgccaacg gcagatccag tttgctattg tcatggagcc ggccattgag
      241 accatcgaga gcgacggcgc gaagctccag cagatattgc tggagctgct taccaatgcg
      301 ctcaagttta ccggaaaagg caagatcgag ctgcgcagcc gccgtcttga ggaaagcgcc
      361 ggcgagtttt tggagatcgc cgtcaccgat accggcatcg gtattcggcg cgaggatcag
      421 caggtaattt tcgaagactt ccgccaactc gacggctcgt cgacccgcca gtatggtggc
      481 acgggtgtcg gacttggcct gtgtaaaaaa ctcgctgcgg ctctgggcgg agagattcgg
      541 ctgcacagcg aggtcggagt gggcagtgtc tttagcctgc gcttgccgct taaatctttg
      601 gggtctcacc caggctcgcc ggctttggct ccggggaccg gtttgctgca atgatctatc
      661 gactgtgaca tattagcggc cagtcgccgg gtgaaaggat aatagccatc atgccaaact
      721 tacgtcaatt agtgatcgcc gccgaagatc cggagcggct cgccaagttt tatcaagagg
      781 ttttcgatct cgaaaagatc gatgacaacc agggggcgct ctttctttcc gacggtacct
      841 tcaacctggg cttgttaccg gaggccgacg cggagaaatg cggctttcgt catcttggct
      901 tcgataccgc gcgtgtcgag tcgatccgca taaagctcgc ccatgtggga ctggcgcaga
      961 gcgaggttag cgaggtgaat tcccccgcga ccatcgatca cgagctcaag gatcccgacg
     1021 gcaataccgt cggtgtatcc cgccgcgcct tcgatgtcgc ctacacccgc cgaccggtgc
     1081 cgattcgtca cattgcgctg tacacgccca acccgcagaa gatggctgag ttttattgca
     1141 aagtgctcga catgaaggaa gtcgaacgca ctgaccgctc gtcgattttt gtttccgacg
     1201 gctattttaa tctagcattg ctctaccagc gcaaggaaga gcctatgggc ctcaatcatt
     1261 ttggttttca cgtgcagagt aacgaggaaa tgcagacccg cgccgaaaga gccggcgtgc
     1321 gccgtggcgc caagcggccc gaccgtattc cgttcgccga gtaccgggtc catgatccgg
     1381 aaggtaacgg catcgatatc tcgcagaagg gttggcgggt ctaaacatta agaacagaaa
     1441 aggttcaaac cgttccagtc gttcaaaatg ttcaaaacgt ttcggaaaac attgacgatg
     1501 ttacgtgact atgaacggtg acgaattctt acaagaaatc ctcgcgccgg tgcatgagtt
     1561 ttgccggatg cttgatggca ggccgccggc ctattcctat gtgccgctgc gcaccgacga
     1621 agaacgcatc cgggtgatgc aaagccgtct cttcaatgaa ctgcgcgcgg cagatttatt
     1681 tggcacatgg ttaaagacca cgcccgagct tgacgtcaaa gcgaccatgg ccgagtcggc
     1741 ccatgaagag atggttcacg cgcaattatt gagtaagcgt atccgcgacc gcggcgctga
     1801 gccctttgac taccgaccgt tgccggtgca gattgccatg ttcaatgcca tggaggggtt
     1861 gaacgatacc tgtgaacgca tcgccggttt gtcgctggcg ggggaaacgg tggcaaacta
     1921 cttgattcag atgtgcctca aggcgccgga tgtgccggaa tggatcaagc aaccctatag
     1981 gaagattacc gaggacgagg aagggcatgg cagtgtgccg caaatctttc tcaaacgcta
     2041 cgccaccact gaagaacgcc aagatcgggt gcgccgcgcg gtcgccatgc gcatggtgct
     2101 atttcgcgaa tatctcgcca gcctagaccg ctgggcgctg ggccagaatc gctggtaaat
     2161 cggtctgttt tctcgccgct ccgcgctagt ttccgcttgt agtctgaccg ctgcatccgc
     2221 tatatcccct ggtatcgaat tggaggggca atgaagattc tttttttcga cgatttcaaa
     2281 ctcggcgtgc tcaaaggcga caccgtggtg gacgtctccc acgtggtgca agacattcct
     2341 cacaccgggc cccataatct tatcagcggc ttgatcgaaa gcttcaacga ctatcgcagt
     2401 gcgattgaaa aggcggtggc tgcaggcagt ggcgtcgcgc tcaagagcgt gcggatccgc
     2461 ccgccgttgc ccaagccggt caatatcgac tgcatggcgg ttaattacat ggaggacggc
     2521 acgcgcaaag agccggcgcc gatcaacgct tttcataagt cgccaaactc ggtcatcggc
     2581 gacggcgata cgatgattct acctgacgtg ccggcgccga tcttcgaagg tgaagccgag
     2641 ttagctctgg tgattggcag gcgcgccgac aatgtcactg cagatcaggc gatggagtat
     2701 atcttcggct atatgaattt catcgacggt tcggcgcgtg gtctgccgcc ggccggcaat
     2761 actttttatc agatgaagtc gcgggccact tttgcgccca ccggaccgtt catcgtcacc
     2821 gccgatgaaa tcaccgatcc gcacaatctt caggtccggc tgtggaacaa cggtgtattg
     2881 atgcagagct atcataccag cgacatggcc cacaaaattc cccgctgcat cgaatgggta
     2941 agctcgatcc atccgttgga ggcgggcgat attttggctt tagggacgaa tcaccgcggc
     3001 ctcaatccgt ttcaagacgg cgatgcgatc gagatcgaaa ccgagccgtt gggccgcctg
     3061 catgtaaagg tccgcgatcc gctcaagcgc acttgggcgc gcgagacgcg cttgcagcgg
     3121 caggaaaaag gattggaagg gttcacgccg cagattacgg gtaaatatgc caaagcatag
     3181 acggcgtgtt catggttcgt gatcgtgctc gtgaagaagg cggtagggcc atgatgacgc
     3241 gaacggtttt gctcccccat gatcgccgtc ggtggttcgc ctggcttccc cgccgacatc
     3301 gac
//
LOCUS       DASQKS010000368         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2718984, whole genome shotgun sequence.
ACCESSION   DASQKS010000368 DASQKS010000000
VERSION     DASQKS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718984"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1600)
                     /locus_tag="VE170_09585"
     CDS             complement(98..1600)
                     /locus_tag="VE170_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HYJ15730.1"
                     /translation="MEPDRKWGGSVSFKGAILVALVSLVVGLGISGSLDWLSPGRAVN
                     FLGDAGSPESRPSSDGLPDFVSLARKMKPLVVNISTTQVSEGGRGGGQEFGSPFGDDD
                     QFNDFWKRFFGGPQPRGPQRQRSLGSGFIIDGDGSILTNNHVVENASKIVVKLSDDQE
                     YEAKVIGRDAKTDIAVIKIAAKSSLPAANFGDSDQLEVGEWVVAIGNPFGLDSTVTSG
                     IVSAKGRHIGQGPYDNFIQTDASINPGNSGGPLINLRGEIVGINTAIFSRTGGNMGIG
                     FAIPINLVKELLPQLRGKGKVTRGFLGVLIQKVTPEIADSLGLEKAQGALVANVSKDG
                     PADKAGVKVGDVIVEFDGKEVKDSGDLPIIVARTQIDKKVRMKVVRDKKEMVLGVAVG
                     ELKDEEVVAAAPEKGELGLTVQRLTPQMAESLGLEKSEGVVVSAVEPGSAADDAGIRR
                     GDVLMEIDRRPIRNLDEYRKTVAAIKKGRGVLFLVRRGESTLFLALKPQR"
     gene            complement(1609..2043)
                     /locus_tag="VE170_09590"
     CDS             complement(1609..2043)
                     /locus_tag="VE170_09590"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HYJ15731.1"
                     /translation="MASEEEKPEGKGFTVQDRRRFSPETGEAREDAPEAPESAPQASQ
                     QPTGAPERGENTAQAGALSEIDFSTFIISLSTQALMHLGEIANPVSGKVEADISVAKQ
                     MIDILAMLNEKTRGNLNPSEAQMMESILFDLRMKYVEAVKKR"
     gene            complement(2563..>3138)
                     /locus_tag="VE170_09595"
     CDS             complement(2563..>3138)
                     /locus_tag="VE170_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ15732.1"
                     /translation="VKRLVDATLQRFGAIDILVNNAGSVPAIKFTDVSDVQWYEMLER
                     KLLGYLRMTREVVPHMQKAGWGRIVNVAGTAGWEPSNTGMAVGINNAAVMNWTKSMSL
                     QYGADGILVTTVAPGSIDTPRQVYNRQREAAVTGKSVEELRAARVKEIPLRRLGRPEE
                     VANLVVFLASECSSYMTGTCVTVDGGVVRGI"
BASE COUNT          628 a          990 c          814 g          706 t
ORIGIN      
        1 cccaagtcac ttttttagtg gcggcgcaga tcatggcaac ggcaaaaaat gaggacgcaa
       61 aaaacacgcc ctaaaaagat atccaacgct ggcactctca acgctggggt tttaaggcca
      121 ggaacagggt gctttcgccg cgccgcacca ggaacaagac ccctcggcct ttttttatcg
      181 cagcgacggt ttttcggtat tcgtcgagat tgcggatcgg ccggcggtcg atttccatga
      241 gcacatcgcc acgccgaatc ccggcgtcat cggcggcgct accgggctcc accgccgaaa
      301 ccacaacgcc ttcagatttc tccaacccca agctttccgc catctgcggc gtcagccgtt
      361 gcacggtgag gccgagttcg cccttttccg gcgccgcggc gaccacttct tcatccttga
      421 gctcgccgac ggcgacgccg agcaccattt cctttttgtc gcgcaccact ttcatgcgta
      481 cctttttatc tatctgcgtg cgcgcgacaa tgatcggcaa atcgcccgag tctttcactt
      541 ccttgccgtc gaactcgaca atgacatcac cgactttgac gccggccttg tcggccggcc
      601 cgtccttcga gacgttggcg accaacgcgc cttgagcttt ttccaacccg aggctgtccg
      661 cgatctccgg cgtaaccttt tggatcaaca cgcctaagaa gccgcgggtg actttgcctt
      721 tgccacgcag ctgcggcagt agctctttga ccaaattgat cgggatggcg aagccgatgc
      781 ccatgttgcc gccggtgcgg ctgaaaattg cggtattgat gccgacaatc tcaccgcgca
      841 agttgatcag ggggccgccg gagtttcccg gattgatcga cgcatcggtc tgaatgaaat
      901 tgtcgtaagg accctggccg atatgccggc ctttggcgct gacgatgccc gacgtcacag
      961 tgctgtccaa gccgaacggg ttgccaatgg caaccaccca ttcgcccact tcaagctgat
     1021 cggaatcgcc aaaattcgcc gccggcaatg acgacttggc ggcgatcttg atcacggcga
     1081 tgtcggtctt ggcgtctctt ccgatgactt tggcttcgta ttcttgatcg tccgaaagtt
     1141 tcacgacgat cttcgaagca ttttcaacta catgattatt ggtcaatatc gaaccgtcgc
     1201 cgtcgatgat aaatcccgag ccgaggctgc gttgacgctg cggcccgcgc ggctgcggcc
     1261 cgccgaaaaa acgtttccag aaatcgttga actgatcgtc atcgccgaaa gggctgccaa
     1321 actcctggcc gccgccacgg ccgccttcgc tgacctgagt cgtggagatg ttgaccacca
     1381 ggggcttcat ctttctcgcg agagagacga agtccggcag gccgtccgat gatgggcgcg
     1441 attccggaga accggcgtcg ccgaggaaat tgaccgcccg tccaggcgac aaccagtcga
     1501 ggctgccgct aattcccagt cccaccacca aagaaaccag ggcgacaagt atcgcgccct
     1561 tgaaacttac gctgccgccc cacttacgat ccggttccat ccccactcct atcgcttctt
     1621 caccgcttcg acatatttca tgcgtagatc gaaaagaata ctttccatca tttgcgcttc
     1681 gctcgggttc aaattgccgc gggttttttc attcagcata gccaagatat cgatcatttg
     1741 cttggccacg ctaatgtcgg cttcgacctt gccgctaacc gggttggcga tttcgcccaa
     1801 atgcatcaac gcctgggtgc tcaagcttat gatgaaggtc gaaaaatcga tttccgatag
     1861 agcaccggcc tgagcggtat tctccccgcg ctccggtgcg ccagtgggct gctgcgatgc
     1921 ttgcggcgcc gactccggcg cctctggcgc gtcctcgcgc gcttcgccag tctccggcga
     1981 aaatcgccgg cgatcctgca ccgtgaatcc ttttccttct ggcttttctt cttctgacgc
     2041 catttcccta ctccggcaaa ccgttcaata aaaaaatctg gaaaccaacc cgcggacaga
     2101 caaacggaga ttcttgaact actcggtcta cttgagtttt gtcacggtaa aattggcttg
     2161 cagaatggtc gcgatgcaga cataaaactc ggttcaaact gtaataccca cccctagcct
     2221 tgtcaagtta tcgactcgcc ctgggacact cccggcatga ttttcgcctg ggcataaacg
     2281 gcagtgacaa tcttgatccg tttgaatacg agcggcgtca aaaaactcgg tctcaaagcc
     2341 gaagttggag tcgccgctgc gtaattggag caaatatacc gcgacgactt tcagccggag
     2401 ggcggaccgt gcgcgccatc agcgaccacg cgcccactcg cgcttgcttg tgtgcatggc
     2461 cttaaggaag acacgcgctc agcggcctgc cttcgctggc cgcaagacga tcgctttgtc
     2521 tatcaccgcc tcaatgtcgc ttggcgtcgg tgcgacccga agtcaaatcc ctctcaccac
     2581 gcctccgtcc accgtcacac aagtgccggt catataactc gaacactccg aagcgagaaa
     2641 gacgacgagg ttggccactt cttctggccg gccgagtcgg cgtagcggaa tctctttcac
     2701 ccgtgccgct cgcagctctt cgacgctttt cccggttact gccgcttcgc gctggcgatt
     2761 gtaaacttgc cgcggcgtgt cgatggagcc cggcgccacc gtagtcacca agatcccgtc
     2821 agcgccatat tgcagcgaca tcgattttgt ccagttcatg acggctgcat tgttaatgcc
     2881 gaccgccatc ccggtattgc tcggctccca tcctgcggtg ccggcgacgt tgacaatgcg
     2941 cccccagccg gctttttgca tatgcggtac tacctcgcga gtcatgcgca ggtagcccag
     3001 cagttttcgc tccaacattt cgtaccactg gacgtcgctg acatcggtga acttgattgc
     3061 cggcacgctg ccggcgttgt ttaccaatat gtcgatggcg ccgaaccgtt gcagagtcgc
     3121 gtcgaccagg cgcttgac
//
LOCUS       DASQKS010000369         6862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1044717, whole genome shotgun sequence.
ACCESSION   DASQKS010000369 DASQKS010000000
VERSION     DASQKS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044717"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /gene="ilvB"
                     /locus_tag="VE170_09600"
     CDS             complement(<1..1415)
                     /gene="ilvB"
                     /locus_tag="VE170_09600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HYJ15733.1"
                     /translation="MQLTGAQLITVLLERQGITTIAGIPGGANLPLYDALQASSIRHI
                     LARHEQGAGFIAQGMARVTGRPAVCLATSGPGATNLLTAIADAKLDSIPLVAITGQVP
                     RNMIGTDAFQEVDTFGLSLPITKHNILVRSALELRDVIPNAFRIAASGRPGPVLVDVP
                     KDVQTELVDVDSLPEPARAQPAPLCPADDLLRAAAMINAAQKPLLLIGAGIIAAGASK
                     DLRRLMEKASIPAAATLLALGALPHDHPLYLGMVGMHAARFTNMLLDECDLLVGLGVR
                     FDDRATGKAAEFCPRARILHIDIDPSELGKIKQPSLGLVADVGDALAALLPMIAANRR
                     CQWTQHIDRVRACYPLAMPGADDPLRPYGIVRTVASLLPDDSIVTTDVGQHQMWVAQA
                     YPFKRPRQWLTSGGLGTMGFGLPAAIGAALACPGRTVACFSGDGSLLMNLHELATAAE
                     EGVEVKIILLNNSHLGMVRQQ"
     gene            complement(1936..2328)
                     /locus_tag="VE170_09605"
     CDS             complement(1936..2328)
                     /locus_tag="VE170_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYJ15734.1"
                     /translation="MAKAALNPDGLPVPRGSYSLVNIAQPGRMVFIAGQTASDHEGKV
                     VGPGDARAQTRFILGKIQRAVEAAGGTMNDVVALNVFTTDVRNHRDVNETRREVLGSN
                     FPTSTMVQVVALARPELLLEINAIAVIS"
     gene            2732..3898
                     /locus_tag="VE170_09610"
     CDS             2732..3898
                     /locus_tag="VE170_09610"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ15735.1"
                     /translation="MGELMRRYWIPALLSEEIPEPDCPPVRVSILSEQLVAFRDSQGR
                     VGLLAEHCSHRGTSLFFGRNEECGLRCIYHGWKYDTEGHVLDTPAEPAGSDFKTKLKH
                     VAYPTVEAAGIVFAYLGPRDKMPLFPNYEWALLPKEQTYVTKVLMDCNYLQGLEGECD
                     SSHLSFLHREFSATGRRQLFALDSAPAYETEETDFGVRLIATRNAGPDSQYVRVSSFV
                     MPVACWVPARNREAHIYVPVDDEHAWRYDLGFLQDRKPTVEDWNRQSQIGPDYRKIRN
                     LQNNYLQDRQLQATVNYTGIENFLNHDGCATETMGPIFDRSREHLGVSDKAVIAVRKY
                     LINTVKEFQDGKEPPHMVRDPARNLFPHIDTFARTIPVNVHWRDRFAHLKLSAM"
     gene            complement(3947..5170)
                     /locus_tag="VE170_09615"
     CDS             complement(3947..5170)
                     /locus_tag="VE170_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ15736.1"
                     /translation="MKRSNDRILTTHTGSLPLPAPVRQALAAKRRGEAYDQGAIDATL
                     TTAIGEVVRRQKEVGIDVVNDGELSKTAWNDYVVHRVSGLERKPRSRARTSEPWSVAQ
                     GIVRPRNTGLVRGDLDGAPRHYRADIAEFYDLARAQSDAHDNGGMAYVCTGPIGWTDF
                     AAVETDLDRLASSVAATQPHDVFMSALSPGCFARFFRNEFYPNDETYLQAVADAMHLE
                     YQAIVDAGFVLQLDCPDLASGANTDYAGLSIKQFRKVIERHVECLNYALRTIPREQVR
                     IHVCWGNYTGPHHRDIALRDVIDIVLKANVTGITIESANPRHGHEWKIWQDIKLPEGK
                     ILFPGVIDDLSYFIEHPELVAERIVRFAKLVGKENVIACTDCGLRHLPDASLAFAKLY
                     ALAEGAKLASQELWG"
     gene            5533..6180
                     /locus_tag="VE170_09620"
     CDS             5533..6180
                     /locus_tag="VE170_09620"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HYJ15737.1"
                     /translation="MASIGKAKQRCPKCDSSLTLCMCAVLPRLDLRTKVTLVIHRHEL
                     LRSSNTGMLARRALVNSELRIRGESREMLDLSDLLSSHYRSLLFYPSADAVELTPALV
                     DQDPRPIQLIVPDGTWRQSRKIHSRHHELKSVPRVKISTPNDSTFQLRAQSRREGMAT
                     LLAIAAALAIIEGETVAGQLIMLYQARVTRTLRARGLLPQISESSAASSVRSKVS"
     gene            complement(6282..>6862)
                     /locus_tag="VE170_09625"
     CDS             complement(6282..>6862)
                     /locus_tag="VE170_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ15738.1"
                     /translation="VYSPTQKNREAYAKEMGNKLGIEIIAVDKPEEILRGSDIVATCT
                     DSIQVLISDPDQIESGMHLTCVKANEWSPEIVTQSDVVIRMGRPTLNLDVGQLRIGGE
                     AAVVAGNAEEIKRIANPKVDVFSRDYPLLTDIMSGKLKGRTDNKQVTFFANSGTQGLQ
                     FASTAGYVVREAKKRGLGQQIPTDWFLQDIRD"
BASE COUNT         1366 a         1926 c         2088 g         1482 t
ORIGIN      
        1 tgctgctggc gcaccattcc caagtgagag ttgttgagca ggataatttt gacttcgacg
       61 ccttcttccg cggcggtggc aagctcgtgc aggttcatga gcaagctgcc gtcgccgctg
      121 aaacaagcga ccgtgcggcc aggacatgcg agtgcggcgc cgatggcggc ggggaggcca
      181 aagcccatgg tgccgaggcc ccccgaagta agccattggc gtggccgttt gaatggatag
      241 gcttgcgcca cccacatctg gtgctgcccc acgtcagtcg tgacgatgct gtcgtcgggc
      301 aacaaagagg cgacggtgcg gacaatgcca taaggccgca aaggatcgtc cgcgccgggc
      361 atcgccaacg gatagcacgc gcgaaccctg tcgatatgtt gcgtccactg gcaacgccta
      421 ttcgccgcga tcatcggtag cagagcggcc agcgcatcac cgacatcggc caccagcccc
      481 aggctcggct gcttgatctt gcccaactcg cttggatcga tgtcgatgtg gaggatcctg
      541 gcgcggggac agaactccgc cgctttgccg gtagcccggt cgtcgaagcg tactccgagg
      601 ccgaccagca ggtcgcattc gtcgagcaac atgttggtga agcgtgccgc atgcatgcca
      661 accatgccaa ggtagagggg atggtcatgg ggcaacgcgc cgagagcgag caaggtggcg
      721 gccgccggaa tggaagcctt ttccatcaag cgccgcagat ctttcgatgc gcccgccgcg
      781 atgattccgg cgccgatcag cagcagcggt ttttgcgccg cattgatcat ggcggcagcg
      841 cgaagcagat cgtcggcggg gcacagcggc gctggttgag ctcgtgcggg ctcgggaagc
      901 gaatccacgt cgaccagttc ggtctgcacg tccttcggaa cgtccaccag cacgggcccc
      961 ggtcggccgg acgccgcgat acgaaaggca ttgggaatga cgtcgcgcaa ttccagggcg
     1021 gaacggacca aaatattatg tttggtaatc ggcaagctga gcccgaaggt gtcgacctct
     1081 tggaaggcat cggtaccgat catgttgcgg gggacctggc cggtaatcgc cacgagcgga
     1141 attgaatcca acttggcatc agcgatggct gtgagcaggt tggtggcgcc gggccccgag
     1201 gtggcaaggc acaccgccgg ccggccggtg acccgtgcca ttccctgtgc gatgaacccc
     1261 gcgccttgtt cgtgacgcgc caatatatgg cgaatcgaac tggcttgcag cgcatcgtac
     1321 aggggtaggt tcgcgccgcc ggggatgccg gcaattgttg tgatgccttg gcgctccagg
     1381 agcacggtta tcagttgggc acctgttagt tgcatgggag ctctcctatc tggatttgct
     1441 ccctgcgcgg cgtgcggaga cggtgggtgg gccaaaaaaa caaaaccccc gtcggccatc
     1501 gcgccggcag gggtttaaaa attgtttaca attttcccgc tacgacgcga aagcctcgct
     1561 tactaagacg actacgacta cgaaatgcaa aggccgcgtc tttaacgcac ggggcttgtg
     1621 ggtctcagcg gtgaacatga ttggaaacta gcatcaaatg gatcgcaccg caaatcggag
     1681 tgattttttt gggctcagct tttgtctggt agtgtaagtt ctagacggaa ccaagcttga
     1741 cgatctctat ggccttcgcc gggatacatc atcggactct cccgcgccag acaaacaaac
     1801 cgcgcctctg tgcgcgacct atcgctgcca ctcgtcgcgc ttacatcgag tttttaccgt
     1861 ttaagtcgcg cacgcggctg aacgcttcaa ctatcgtggc gaaaccaatc ttttgggttt
     1921 ttatcccgat tgctttcagg aaataaccgc gatcgcattg atctcgagca gcagctccgg
     1981 tcgcgccaaa gccaccacct gcaccatcgt gctggtcggg aagtttgatc ccaagacttc
     2041 acgccgagtt tcgttgacgt cgcgatgatt acgcacgtcc gtggtgaaga cgttgagcgc
     2101 gacgacatcg ttcatcgttc caccggcggc ttcgacggca cgttgaattt tacccaagat
     2161 aaagcgcgtc tgtgcccgtg catctcccgg gccgacgacc ttgccctcat gatctgaagc
     2221 cgtctggccc gcgatgaata ccatccggcc cggctgcgca atgttgacca gcgaataact
     2281 gccacgcggc accggcagtc catcgggatt gagtgcggcc tttgccattg gaatgtcctc
     2341 cttctaatca attgagctgt agacctttaa cgcattatga gcaacaagca tggaccagtc
     2401 tattgcctgc tggaacccac tcgcctacgg tcctgctgct tcgaagttat cccaaaatgt
     2461 tagggcccag caaggttaaa gagcttgcag tcataatttg cgtgattcga ttgccggatt
     2521 tagccacggt gtttcgcggg tgctcacacc aaggccgctg attccttggc ttttcatgga
     2581 acgcctctac gatcaagagt aaaaggagca ccgcgcccgc gagtgttgga aattgttgat
     2641 tgaatgagta ttgtgcccgg cggaggtgag ttgctaaatg cttcctcgag aacacaacga
     2701 acttttgacc aggaccgcag cggggacccc gatgggggag ttgatgcggc gctattggat
     2761 tcctgcgctg ttatccgagg agatcccaga acccgattgt ccgccggtgc gagtgagcat
     2821 cctcagcgag cagctggtgg cctttcgtga tagtcaggga cgcgtcgggc ttttagcaga
     2881 gcattgttcg catcgcggca cgtcgctatt ttttgggcgc aacgaggagt gcggtctccg
     2941 gtgtatttac cacggctgga agtacgacac ggaaggtcac gtgctcgaca ccccggcgga
     3001 accggccggg agcgacttca aaaccaagct taagcatgtc gcctatccga cggtggaagc
     3061 ggcgggcatc gtctttgcct atctggggcc gcgcgacaag atgccgctgt ttccgaacta
     3121 cgaatgggcg ctgttgccga aagaacagac ctacgtcact aaagttctaa tggattgcaa
     3181 ctatctgcag ggacttgaag gcgaatgcga ttcgtcgcac ctgtcgttct tgcaccgcga
     3241 gttttccgct accggccgcc ggcagttatt cgccttggac tcggcgccgg cctatgaaac
     3301 cgaggagacc gacttcggcg tgcgcttgat cgcgacgcgc aatgccggac ccgacagcca
     3361 gtacgtgcgg gtgtcgagct tcgtcatgcc ggtcgcttgt tgggtgccgg cgcgcaaccg
     3421 ggaggcgcat atctatgtgc ctgtcgatga cgaacacgca tggcgctacg atctgggctt
     3481 tcttcaggac cgcaagccga ctgtggaaga ctggaatcgc caaagccaaa tcggccccga
     3541 ctaccgcaag atccgcaatt tacaaaataa ctatctgcag gaccgccaac tccaggccac
     3601 cgtgaattac accggcatcg aaaactttct caaccacgac ggctgcgcga ccgaaaccat
     3661 ggggccgatc ttcgatcgct cgcgcgagca cttgggcgtg agcgacaagg cggtgattgc
     3721 cgtgcgcaaa tacctgatca ataccgtcaa ggagtttcag gacggtaaag agccgccgca
     3781 catggtgcgc gatccggcgc gcaatctctt tccgcatatc gacaccttcg cgcgaacgat
     3841 tcccgtcaac gttcactggc gcgatcgttt cgctcatctg aagttgtcgg cgatgtaaat
     3901 tcgttcccgc cctgcccgcg accaatcccc cggagtaagg ccatggctag ccccatagtt
     3961 cttggctcgc cagcttggct ccttcggcga gcgcatacag tttagcgaat gccaggctgg
     4021 catccgggag atggcgcaag ccgcagtcgg tgcaggcgat gacattttct ttgccgacca
     4081 gtttggcaaa acgcacgatg cgctcggcga cgagttcggg atgttcgatg aaatagctga
     4141 ggtcgtcaat cacgccgggg aagagaatct tgccttcggg caattttata tcctgccaaa
     4201 tcttccactc atggccgtgg cgcggattgg ccgattcgat ggtaatgccg gtgacgttgg
     4261 ctttgagcac gatatcgatg acgtcacgaa gtgcgatgtc gcggtggtgc ggccctgtgt
     4321 agttgcccca gcagacgtgg atgcgcacct gttcgcgtgg aatcgttctc agcgcatagt
     4381 tgaggcactc gacatggcgt tcgatgactt tgcgaaattg cttgatgctc aggccggcgt
     4441 agtccgtgtt ggcgccggag gccaagtccg gacagtcaag ttgcaagacg aaaccggcat
     4501 cgacgatggc ctggtactcc aagtgcatgg catccgcaac ggcttgcaga taggtttcgt
     4561 cgtttggata gaattcgttg cggaaaaagc gcgcgaaaca gccgggcgag agtgccgaca
     4621 tgaagacgtc atggggttgg gtcgctgcga ctgacgaggc cagccggtcg aggtcggttt
     4681 ccaccgcggc gaaatccgtc cagccgattg gaccggtaca gacgtaagcc atgccgccgt
     4741 tgtcatgggc gtcgctctgg gcgcgcgcca gatcgtaaaa ctcggcgatg tcggcacggt
     4801 agtggcgcgg cgcgccatcg aggtcgcccc tcactagtcc agtattgcgc ggccggacga
     4861 taccttgagc aacgctccag ggctcagagg tgcgggcgcg cgatcgcggt tttctttcca
     4921 ggccgctgac ccggtgcacg acatagtcat tccaggcggt tttgctgagc tcgccgtcgt
     4981 tgacgacgtc gatgccgact tccttttggc gccggacgac ctcgccgatg gccgtggtca
     5041 gggtggcgtc gatcgctccc tgatcgtaag cttcgccgcg tcgtttggcg gcgagggctt
     5101 ggcgcaccgg cgccggcagc ggcagactgc cggtgtgggt ggtgaggata cgatcgttac
     5161 tgcgtttcat ggaaaatgcc tcggcgttaa tggagcttcg aactcatgct aactgagcca
     5221 tagtgcgcac cgattcattt tatatcacgt tgccatcgct ggactttgag cggtcgaagg
     5281 tgcctgctca gtaccctggt tatttatgcc atcgtggttg tacttattcc ggcgggcgga
     5341 tttttacaag caaaggaaat ctgtcagcgg gcatccgggt tatctttcaa gtcaatgcgt
     5401 ttttcaaccg tacgttcgcc acctggatcc ggcttgcgtc ggaatgatgc gggcggcccg
     5461 acatccgcgc gacaaactgt cgatggcttt gtcctgtctg ggaagttata acgttgtcga
     5521 ggaaaactat cgttggctag cattggcaaa gcaaaacagc ggtgcccgaa atgcgattct
     5581 tcgctgacgc tgtgcatgtg cgccgtattg ccgcgcttgg atttgcgcac gaaggtgacc
     5641 ttggtgatcc atcgtcacga gctgttgcgc agcagcaaca ccggcatgct ggcgcggcga
     5701 gcgctggtca acagcgagtt gcgcatacgc ggcgagagcc gggagatgct cgatctgagc
     5761 gatttgttgt cttcccatta tcggtcattg ctgttttacc cgagcgcaga tgccgtcgaa
     5821 ctcactccgg cgctggtgga ccaagatccg aggccgattc agttgatcgt gccggacggt
     5881 acttggcgcc aatcgcgcaa gattcattca cgccatcacg agctcaaaag cgtgccgcgg
     5941 gtgaagatca gcacgcccaa cgattcgacc tttcagctac gagctcagag ccggcgggaa
     6001 gggatggcga ccttactcgc aatcgccgcg gcgcttgcaa tcatcgaggg tgaaacggtg
     6061 gcggggcagt tgataatgct ctatcaggca cgggtgactc ggacgctcag ggcgcgcggc
     6121 ctgctgccgc aaatttccga gtcgagcgct gcttcatccg tgcgttcgaa ggtttcctag
     6181 gagcccttcg acgggctcac aacgaacaga gaaatattag cgcgcgcgaa atcttcggct
     6241 atacgcagca ttgtggaaat cggcgattac ttcagctgca ctcagtcgcg gatatcctgt
     6301 aaaaaccaat cggtgggaat ctgttggccg agaccgcgtt tcttggcttc gcggacgacg
     6361 tagccggcgg tcgaggcaaa ctgcaatcct tgggtgccgc tgttggcgaa gaaagtgact
     6421 tgtttgttat cggtgcggcc tttgagcttg ccggacatga tgtcggtgag cagcggatag
     6481 tctcgggaaa agacgtcgac cttaggattg gcgatgcgct tgatttcttc agcgttgccg
     6541 gcgaccaccg cggcttcgcc gccgatgcgc agttgcccga catcgagatt caatgtcggg
     6601 cggcccatgc gaatcactac gtcggactgc gtgacgatct ccgggctcca ctcgttggct
     6661 ttgacgcagg tgagatgcat gccggattcg atctggtcgg gatcgctgat cagtacttga
     6721 atcgaatcgg tgcaggtggc gacgatgtcc gagccgcgca ggatttcttc cggcttgtct
     6781 acggcaataa tttctatgcc caatttattg cccatttctt tggcgtaggc ttcacgattc
     6841 ttctgagtcg ggctgtagac tt
//
LOCUS       DASQKS010000370         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4810937, whole genome shotgun sequence.
ACCESSION   DASQKS010000370 DASQKS010000000
VERSION     DASQKS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4810937"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1083
                     /locus_tag="VE170_09630"
     CDS             121..1083
                     /locus_tag="VE170_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15739.1"
                     /translation="MVTPLDPVIAEIIPKLPLRDPETMTPQSARDALRALAAARAAVP
                     PPPVASAIDTEVKGAAGQIAARVYRVSDAKSPTVVFFHGGGWVAGDLETHDRQARLLA
                     IETGAVVVSVDYRRPPETRFPGAFEDAFAAVRDVFARIAAFGGDSARVGVAGDSAGGN
                     LAATVAIACRDAGIKLAAQLLAYPVTDVAGNFADATENARFPSRVENAKGYFLSRAVM
                     EWFCGHYLDDASHGTDWRASPLRAKSLAGLAPAVVTTAWFDPLRDEGKAYAEALTAAG
                     VTTKYHHGPGLIHGYFGLGDASEAARGEAQRARADFRAMLERGA"
     gene            1163..2665
                     /locus_tag="VE170_09635"
     CDS             1163..2665
                     /locus_tag="VE170_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYJ15740.1"
                     /translation="MKLTVSNAPLRAVACIGLILLAFGLIFLQLAGIEISKKIAAAGL
                     SHQQGHAYTATIGLEARADNAKTPNRSTVALVENGNRIGVPHSLHSEIGNLGAGRYSH
                     WGDSLIFSSSDGSSPATNGRIYEAAYRWRPYPAIALPLLVLMLYVSARLTYAGKVFSI
                     NIAVLAFFAALSLAALEIALRKTTLFDQLSDPTPFYFPKYLVEADRLISKTGFIDVNG
                     FRLNEKADDLIESLKAERGCKIVVLGDSFVWGDGLYPEVRWTSKLDKMLNCRVFPFGR
                     NGWSTVEYLGFYEQHLRRLDFDYLLISIVENDPHPRGRFSAYNFPPNFMPQRQNRFDL
                     ALMLNAMDYKFVLEESFAYRYLNSLVKAAGNSLPLERGSATAPPIITHGYAGWLDRLY
                     KDDVYSIWESVLRDFKSVANHKYGFLLTPHDLAGNRKIFWSQITKTMTGNNFLYESAY
                     PDIIKLFGDQARPKESWANPANGHPGDATTTIYADKALRLLERLGYKQAQ"
     gene            complement(2757..>3693)
                     /locus_tag="VE170_09640"
     CDS             complement(2757..>3693)
                     /locus_tag="VE170_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15741.1"
                     /translation="SAPAIAQAKTKVRVGYLHTVAVDGQIWTGMDRGTFDKQGLNLEL
                     RQFNTGLEIFQALIGGSLDVLATGAVLSNFPARGQGKVFLINDIEVATAQLWVRSDQG
                     IKSFADLKGKRIATATGTTAHVFLDKALRANNIDPKEVELVNQTMPAAVTAFISGAVP
                     AVALWVPFNVTVRDKVPGAVKLADASAYYPQAAIIGGWAAANDYYEPNKETLGKLIKG
                     WAEANDYIIANPAEAMEKLQKGHYSQTPLADINESFKAQKMFTSSEWKKYYSDGTVTN
                     WLQQTTDFFMANANIKDFTPATKYFDPSLYLKTIA"
BASE COUNT          701 a         1077 c         1118 g          797 t
ORIGIN      
        1 cgcctttttc aattcgtcgt tttcggtggc ctcccatgcg gcggtggata cggcggcggc
       61 gttgccggag cggcgagtgg aacctatgct ggtggaaaat acctgccccg ggggaacacc
      121 atggtcacac cgctcgatcc cgtcattgcc gagatcattc caaaactgcc gttgcgcgat
      181 cccgagacga tgacgccgca gagcgcccgc gatgcgctac gtgcgcttgc cgcggcgcgc
      241 gccgcggttc cgccgccgcc ggttgccagc gccatcgata cggaagtgaa gggcgcggcc
      301 ggacagatcg ccgcccgcgt ctaccgggtg tcggatgcga aatcgccgac cgtggtgttc
      361 tttcacggcg gcggctgggt cgccggcgat ctcgaaaccc atgaccgcca ggcccggctg
      421 ctcgcgatcg agaccggcgc cgtggtggtt tcggtcgatt accggcgtcc gccggaaacg
      481 cgctttcccg gcgcgttcga ggacgccttc gccgccgtgc gcgatgtgtt cgcgcgcatt
      541 gccgcgttcg gcggcgacag cgcgcgcgtc ggcgtggctg gcgacagcgc cggcggcaat
      601 ctggcggcga cggtcgcgat cgcctgccgc gatgcgggga tcaagcttgc cgcgcaattg
      661 ctggcctatc ccgtcaccga cgtcgccgga aattttgccg acgcaacgga gaacgcgcgc
      721 tttccctccc gcgtggagaa cgcgaagggc tatttcctgt cacgcgcggt gatggaatgg
      781 ttctgcggcc attatcttga tgacgccagc catggcacgg attggcgcgc ttcgccgcta
      841 cgcgcgaaaa gtcttgccgg ccttgcgccg gcggtggtca cgacggcgtg gttcgacccc
      901 ttgcgcgatg aaggcaaagc ctatgccgag gcgctgactg cggccggtgt caccacgaag
      961 taccatcatg gtcccggcct gatccacggc tatttcggtc tcggcgacgc gtcggaggcc
     1021 gcacggggcg aagcgcaacg cgcccgtgcc gatttcaggg ccatgctgga acggggtgct
     1081 tagacgcggc aagcttatgc gcagcttcag cgaaggacgg tcggacttcc ggctttactg
     1141 aagacaggct ataaactgca acatgaagtt gacggtatca aatgcgccgc tgcgcgcagt
     1201 cgcctgcatt ggccttatcc tgctggcgtt cggtctgatc tttctgcaac tggccggaat
     1261 cgagatttcc aagaaaatag ctgctgcagg actctcgcac cagcaaggcc atgcatatac
     1321 ggctaccata gggctcgaag cacgggccga taatgccaag accccgaaca ggtcgacggt
     1381 agccctggtg gagaatggaa atcgaatcgg tgtgccgcat tcacttcact cggagatcgg
     1441 aaaccttggc gcgggacgct actctcattg gggagacagt ctcatatttt cgagcagtga
     1501 cggctcaagc cctgcaacca acggcaggat ctacgaggct gcgtatcgat ggagacctta
     1561 tccagccata gctttgcctt tgctcgtatt gatgctctac gtctcggcgc gactcaccta
     1621 cgcgggaaaa gttttttcta tcaacattgc cgttctggct tttttcgcgg cgctttccct
     1681 ggcggcactg gaaattgcgc tgaggaagac gacattattc gaccaactgt cggatccgac
     1741 acctttctat ttccccaaat acctggtcga ggccgacagg ctcatcagca agacgggatt
     1801 catcgatgta aacggatttc gattgaatga gaaagccgat gacctcatcg agtcattgaa
     1861 ggccgaacga ggttgcaaga ttgtcgtcct gggtgacagc tttgtctggg gagacggcct
     1921 ttatcccgag gtcagatgga caagcaagct cgacaagatg ttgaattgca gggtttttcc
     1981 gttcggaagg aacggctgga gcaccgttga gtatctcggt ttctacgaac agcatttgcg
     2041 gcggcttgat ttcgattatt tgctcatttc gatcgttgag aacgatcctc atccgcgagg
     2101 gcgcttttcg gcttacaatt tccctcccaa cttcatgcca cagcgccaaa acaggtttga
     2161 tcttgcattg atgctcaacg ccatggatta taaattcgtt ctggaagagt catttgccta
     2221 tcggtacttg aattcgcttg ttaaggcggc tggaaattcc ttgccgcttg aacgcggatc
     2281 agcgactgct ccacccatca tcacccatgg ctacgctggc tggctcgatc gactgtacaa
     2341 ggatgacgtc tattcgatat gggagtctgt gttgcgtgat ttcaagtccg tcgccaatca
     2401 caagtatggc ttcctgctta cgccgcatga tctggcgggc aaccgaaaaa tattctggag
     2461 ccaaataaca aaaacaatga ccggcaacaa ttttctctac gagagtgctt atcctgatat
     2521 catcaagttg tttggagatc aggcccggcc aaaggaatcc tgggccaatc ctgcgaacgg
     2581 gcacccggga gacgcgacga ccacgatcta tgccgacaag gcgcttaggc tgcttgagcg
     2641 gcttggctac aagcaagcgc agtgaaccag aggcgctcgc acgccttgta ttgccgcctc
     2701 atgagacggc tgcgtagcga acggcgactg caagccgccg ttcacgagag cctgagttac
     2761 gcaatcgtct tcagatagag gctcggatcg aaatatttgg tggccggcgt aaaatccttg
     2821 atgttggcgt tggccatgaa gaagtcggtg gtctgttgca gccagttggt caccgtgccg
     2881 tccgaatagt atttcttcca ttcgctcgac gtgaacatct tctgggcctt gaaggattcg
     2941 ttgatatcag ccagcggcgt ctggctgtag tggcccttct gcagcttttc catcgcctcg
     3001 gcgggattgg cgatgatgta gtcattggct tcggcccagc ccttgatcag cttgcccagc
     3061 gtttccttgt tcggctcgta gtagtcgttg gccgccgccc agccgccgat gatggccgcc
     3121 tgcggataat aggccgaggc gtcggccagc ttcacggcgc ccggcacctt gtcgcggacg
     3181 gtgacattga acggcaccca cagcgccacc gccggcaccg cgcccgagat gaacgcggtc
     3241 acggcggccg gcatggtctg gttgacgagt tcaacctcct tggggtcgat gttgttggcg
     3301 cgcagcgcct tgtcgaggaa cacatgggca gtggtgccgg tcgccgtggc gatgcgcttg
     3361 cccttcagat ccgcgaacga cttgatgccc tggtcggagc gcacccagag ctgcgcggtc
     3421 gccacttcga tgtcgttgat cagaaacacc ttgccctgtc cgcgcgccgg aaaattcgac
     3481 agcaccgcgc ccgttgccag cacgtcgagg ctgccgccga tcagagcctg gaaaatctcg
     3541 aggccggtgt tgaattggcg cagttccagg ttgaggccct gcttgtcgaa ggtgccgcgg
     3601 tccattccgg tccagatttg cccgtcgacg gcgaccgtat gcagatagcc gacgcggacc
     3661 ttggtcttgg cctgggcgat ggctggcgcc gaa
//
LOCUS       DASQKS010000371         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1455000, whole genome shotgun sequence.
ACCESSION   DASQKS010000371 DASQKS010000000
VERSION     DASQKS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455000"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VE170_09645"
     CDS             <1..547
                     /locus_tag="VE170_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15742.1"
                     /translation="RHNLQPGKDVKFIYLGGVREVLAAIEHGIAAAGVLSSPTTLAAR
                     RLGQKELVNIATLGLPYMHSGVATRRGFLRQQPDRIKAYLRAYIAAIKIANEDAETSK
                     RALRRYLVTSDSAILDEAYQTFRGIFPRVPYVTEDQIKSSLTLSEHPKAAGANPKDFF
                     DTRLLKEIEDSGFVQELYGRR"
     gene            822..1793
                     /locus_tag="VE170_09650"
     CDS             822..1793
                     /locus_tag="VE170_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15743.1"
                     /translation="MIVASLLLIQTSARESFAASANNIRIGYPQPSGAMLPVWVMAEA
                     KLDRKYGVDLQNIYISGGARLTQTLVSGDIELAATGGAVVNAVLSGADLTYVAASVPT
                     YGFSLYVRNDVKDVPSLKGKVVGLMTKGASTDHGLTALLRQYNLRSGQDVKVLYLGGV
                     REGLAALDRGIVSATTISAPTTLMARRMGYKELLNFADLKIPYIQLGIVAHRAFLRQQ
                     PERMKAFLRGYLAAIKVVNEDSDISKRALAKYLATNDGAILDEAYQAYRGIYPKVPYT
                     TEEQVRSILSVADHPKASAADAKEFFDNRFIKELEDSGFIKELYGQR"
     gene            1806..2819
                     /locus_tag="VE170_09655"
     CDS             1806..2819
                     /locus_tag="VE170_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15744.1"
                     /translation="MMTKRNHAAMAILLALAIVMFTHRAHSAAAPDRLVGVHSARVMS
                     QSFPWVAQEAGLFKKHNLDFNLVFISSSAIVTAALLGGDTEMTITGGIGNVAAYVKGS
                     TDIVFTGGVKNVMTQTLVAGGNIKRPEDLKGKRIGVSRIGGNSHYFTIQALRRHGMEP
                     NRDYSFLQTGGDPETLAALMSGSLEVANLTPPTDAQAIARGYHYVIYGPDLKIPYSAT
                     AFVTKRSVMAKRPATVANFMRAMAEASKIVHTDREFYYKVLGKYLRIQDRSVLDSAYN
                     AEIKALEPRLTIKLESLQAILDELTPTDARAKNVKPQEMVDNRYLDEMNKSGFMDQLW
                     SKK"
     gene            2924..>3424
                     /locus_tag="VE170_09660"
     CDS             2924..>3424
                     /locus_tag="VE170_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15745.1"
                     /translation="MPAHAGIQAEVGRKSDNLDSRFRGNDERRRIRLPVDAIGIFRYL
                     SHGCLILAFALLLPGRSRAADKFTTIYSARVMAQALPWIAEDAGLFKKFNLDHNLVFI
                     ASSSIVTAALLGGDPDMTMTGGVGNVIAFVRGSTEIAYVGSAKNLMTQNIIAGGNLKR
                     PEDLKGK"
BASE COUNT          749 a          971 c          992 g          712 t
ORIGIN      
        1 gcgccacaat ctgcaacccg gcaaggacgt gaagtttatt tatctcggcg gcgtgcggga
       61 agtgctcgcc gccatcgagc acggcatcgc cgccgcgggg gtgttgtcgt cgccgacgac
      121 tctcgcggcg cggcgcctgg ggcaaaagga actggtgaat atagccaccc tcggtttacc
      181 ctatatgcac agcggcgtgg cgacgcggcg tggcttcctg cgccagcagc cggaccggat
      241 caaggcttat ctgcgcgcct acattgccgc gattaaaatc gccaatgaag atgccgagac
      301 cagcaaacgc gcgttacgcc gctatctcgt taccagcgac agcgcgatcc tcgacgaagc
      361 ctatcaaacc ttccggggaa tttttccgcg agttccctat gttaccgaag atcaaatcaa
      421 atcgagtctg acgctgtccg aacatcccaa ggcggctggg gccaatccga aggacttttt
      481 cgacacgcgg ttgctaaaag aaatcgagga cagcggtttc gttcaggagc tctatggccg
      541 gcgctgaagg gctatttcgg ctggcgcggg cgaggtttta cagtggcgct gaagtttcgg
      601 tgtttgtgca aaaattccgg cagtcgaaaa ccctgggatt cggatgttcc tcccgccaag
      661 gcgcaaagac gccaagtgac gcgtggagcc cgtcgtcccg agttgaggcg aggggtgcga
      721 aaaagaattc tccctccggt cgaaatgaca ttcttttccg ccctttgcga cttagcgcct
      781 tcgcgggaga tattccggct cgcaggtggg gccgcgcagt attgatcgtt gcgtccctgc
      841 tgctcataca aacatcggcg cgggagagtt tcgccgcgtc ggctaacaac attcgcatcg
      901 gttatccgca accctccggc gcgatgttgc cggtgtgggt gatggcggaa gcgaagctcg
      961 accggaaata tggcgtcgac ctgcagaaca tttacatctc cggcggcgcc cgtctgaccc
     1021 agacgctggt ctccggagac atcgagttgg cggctaccgg cggcgccgtg gtcaacgcgg
     1081 tgttgagcgg cgccgatctg acctatgtcg cggccagcgt gccgacctat ggtttctcgc
     1141 tatacgttcg caacgatgtc aaagatgtcc cttcgctcaa aggcaaagtc gtcggcttga
     1201 tgaccaaagg cgcgtcgacg gatcatggcc tgacagcgct cctgcgccaa tacaacttgc
     1261 gctccggtca ggacgtcaaa gttttgtatc ttggcggtgt gcgcgagggt ttggccgcgc
     1321 tcgatcgcgg catagtctcg gcaacgacga tttcggcgcc aacgacattg atggcgcggc
     1381 gcatgggtta caaggaactg ctcaattttg ccgatctgaa aatcccctac attcaacttg
     1441 gcatcgtcgc ccaccgggct tttctgcgcc agcaaccgga gcgcatgaaa gcgtttctca
     1501 gagggtatct ggcggcgatc aaagtcgtca atgaagacag cgacatcagt aagcgggcgc
     1561 tggctaaata cctggcgacc aacgatggcg cgatcctcga cgaagcttac caggcctatc
     1621 gcggcatcta tccgaaagtt ccctatacca cagaggagca ggtgcgctcg atcctcagcg
     1681 tcgccgatca tcccaaggcg tccgcggcgg acgccaaaga gttctttgac aaccgtttca
     1741 ttaaagagtt agaagatagt ggatttatca aggaacttta cggtcaacgc taaccgggtg
     1801 gtacgatgat gaccaagaga aaccatgcag cgatggcgat tttactggcg ctagcgattg
     1861 tcatgttcac tcacagagcc cacagtgcgg cggcgccgga cagactcgtt ggcgtccatt
     1921 cggcgcgggt catgtcgcaa tcctttccct gggtggccca ggaagccggg ttgttcaaga
     1981 aacacaactt agatttcaat ctggtgttta tttcctcgtc ggccatcgtt accgcggcat
     2041 tgctcggcgg cgataccgag atgaccatca ccggcggcat cggcaacgtc gcggcctacg
     2101 tcaaaggttc gacggatatc gttttcaccg gcggagttaa aaacgtcatg acccagaccc
     2161 tggttgccgg cgggaatatc aaaaggccgg aagacctgaa aggaaagcgg atcggcgtca
     2221 gccgcatcgg cggcaactcc cattacttta cgattcaggc gctgcggcgc cacggcatgg
     2281 agcccaaccg cgattatagt tttttgcaga ccggcggcga cccggaaacc ctcgcggcgt
     2341 tgatgagcgg ctccctggag gtggctaact tgacgccgcc caccgatgcc caagccattg
     2401 ccaggggtta tcattatgta atttacggtc ccgacttgaa gatcccttat tcggcgacgg
     2461 cgtttgtcac caagcggtcg gtgatggcca aacggccggc gacggtcgcc aactttatgc
     2521 gcgcgatggc ggaggcttcc aagatcgtcc acaccgatcg cgaattttat tacaaagtgt
     2581 tgggcaagta tctgcgtata caggaccgca gcgtccttga ttccgcttac aacgccgaga
     2641 tcaaagcact ggaaccgcga ctgacgataa aactcgaatc cttgcaagcg attctcgacg
     2701 agctgactcc caccgacgcc cgcgccaaga atgtcaaacc gcaggaaatg gtcgacaatc
     2761 gttatctcga tgagatgaac aagagcggtt ttatggatca gctgtggagc aagaagtgac
     2821 agggagcttt tctttaagct caatgtcccg cttggcgatg gttggccaac ctcattgttt
     2881 cgacggcgct cccggcatgc tacaggcgaa aaaaacatcc gttatgcccg cgcacgcggg
     2941 catccaggcg gaagtgggaa gaaaatcgga taacctggat tcccgctttc gcgggaatga
     3001 cgaaagaaga agaatccggc tgcccgtcga cgcgataggg atttttcggt atctatccca
     3061 tggctgtttg attctcgcat tcgcattact gctgccgggc cgctcgcggg cggcggacaa
     3121 attcacgact atttattctg cgcgagtgat ggcccaggcg ttgccatgga tcgccgaaga
     3181 tgccggctta ttcaagaaat tcaatctcga tcataacctg gtttttatcg cctcatcgag
     3241 catcgtcacg gccgcgctgc tgggcggcga ccccgacatg accatgaccg gcggggtcgg
     3301 taatgtcatc gctttcgtgc gcggctcgac ggagatcgcc tacgtcggca gcgccaagaa
     3361 cctcatgacc cagaacatca tcgccggcgg caacttgaaa cggcccgaag atttgaaagg
     3421 caaa
//
LOCUS       DASQKS010000372         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_183462, whole genome shotgun sequence.
ACCESSION   DASQKS010000372 DASQKS010000000
VERSION     DASQKS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183462"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..617
                     /locus_tag="VE170_09665"
     CDS             114..617
                     /locus_tag="VE170_09665"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15746.1"
                     /translation="MFRGVDLPPTVPGRLLLHSLPGRYEALERVWHQVKTDAVEAIVC
                     LTERDEIHVKSQEYLQAIDNGSMPCLLLQFGIPDRGAPEDRDAFWHLACDIATRLQSG
                     QTVLIHCAGGVGRTAMLSICVLLALGQAVGDAREVVSRAGSTVETAPQSNLISWCAAR
                     ASGAALT"
     gene            698..1213
                     /locus_tag="VE170_09670"
     CDS             698..1213
                     /locus_tag="VE170_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYJ15747.1"
                     /translation="MDLFLTFSRITLSSFGGALFWARLGLVERQRWLTDREFAEFLTI
                     GQLLPGPNVLNLTVLVGYRLGGWSGAAVSVAGYVGWPFLVVIGMGMLYQQFGALPQVQ
                     QALGGMSKVAAGLLLATVIKLAMALPHRLRPWLFGILAFIAIGVMRWPLLWVVAALAP
                     CAVFLAWKARD"
     gene            1214..1741
                     /locus_tag="VE170_09675"
     CDS             1214..1741
                     /locus_tag="VE170_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYJ15748.1"
                     /translation="MERVDLSALFVHLLALWFVAVGGPSAILPDIHRYVVDVHQLLSN
                     REFAEIYTLAQAAPGPNVMYTTLIGWHLAGWSGALATTIPLLVPAMTLTLFVGHFTER
                     YPDAPILRAIRRGLTPLTIGLMFAGATVLMRAVNHDWRGYLTTVVTAAVVVRLTCNPL
                     WLLAAGALLGIAGVV"
     gene            complement(1757..2323)
                     /locus_tag="VE170_09680"
     CDS             complement(1757..2323)
                     /locus_tag="VE170_09680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HYJ15749.1"
                     /translation="MHVRSICVIFVATLMAFAFAAPSGAAEPPCPPLLDHKFANLMDE
                     PISLCQFRGKVALIVNSASECGYTPQYDGLEKLYRRYRDRGFVVLGFPANDFGGQEPG
                     SNQQIAQFCQVNYGITFPVFAKTAVVGANANALYRELAARTGQPPKWNFHKYLVDKSA
                     QQVTAFASSVEPDDSRLTAQIEKLLAPR"
BASE COUNT          466 a          732 c          833 g          632 t
ORIGIN      
        1 gttttataat cagcgggatt acgggccggt tggcagcggt catggcgtct agattcgcca
       61 gggatcgtcg agggtgaatc tcggtcaaaa cttatttagg gagagttacc ctcatgtttc
      121 gcggtgtcga tctcccaccg actgtgcctg gcaggctctt gctgcatagc ttgccagggc
      181 gctacgaagc gctggagcgg gtgtggcatc aggtgaaaac cgacgcggtg gaagcaattg
      241 tctgtttaac cgagcgcgac gaaatccatg tgaagtctca ggagtatcta caggcaatag
      301 ataacggttc gatgccctgt ttgctcctgc agtttggcat ccccgaccgg ggtgcgccgg
      361 aggatcgcga cgctttttgg catcttgctt gtgatatcgc cacgcgcttg cagtccggac
      421 agactgtctt gatccactgc gcgggcggcg ttggccgcac ggcgatgctt tctatttgcg
      481 tgctgcttgc cctcggccaa gcggttggcg atgcgcgtga agtagtctcg cgcgcggggt
      541 cgactgttga aaccgcgccg cagagcaatc tcatttcctg gtgcgcggca cgggcgagcg
      601 gtgcggcttt aacataaccg gcgttgcacc tgctcagcga agcccaaaca aatagaatat
      661 gcaaccagta gatcccaacc gaccaccgat cacccggatg gacttgttcc tgacattctc
      721 ccggattact ttgtcgagtt ttggcggcgc gctgttttgg gcacggcttg gattggttga
      781 gcggcagcgc tggttgaccg accgcgagtt cgccgagttc ttgaccatcg gacagctcct
      841 gcccgggccc aacgtgctca atctaacggt gttggtcggc tatcgccttg gtggctggag
      901 cggcgccgcg gtttcggtgg cgggttatgt tggctggccg ttcttggtgg tcatcggcat
      961 gggcatgctg taccagcagt ttggcgcgct gccgcaggtg cagcaggcgc tgggcggcat
     1021 gtcgaaggtg gcagcggggt tgttgctcgc gaccgtgatc aagctggcga tggcgctgcc
     1081 gcaccggtta cggccttggc ttttcggcat tctggctttt atcgctatcg gcgtgatgcg
     1141 ttggccgttg ctctgggtgg tcgccgctct cgcgccctgt gccgtgttcc tagcctggaa
     1201 ggccagggat tgaatggagc gcgtcgatct cagcgccttg ttcgtgcatt tactcgctct
     1261 atggtttgtt gccgtcggcg ggccgtcggc gatcttgccg gacatccatc gatatgtggt
     1321 ggatgtgcat cagctgttga gtaaccgcga attcgccgag atctatacat tggcccaggc
     1381 ggcgcccggt cccaatgtca tgtacacgac actgatcggc tggcatctag cggggtggtc
     1441 cggggcgctg gcgacaacca ttccgttact ggtaccggcg atgacgttaa ccttgttcgt
     1501 gggccacttc accgagcgct atcccgatgc gccgattctc cgcgccatcc ggcgcggctt
     1561 gacgccgctc accatcggtt tgatgttcgc cggcgcgacc gtgctcatgc gcgctgtgaa
     1621 tcatgattgg cgcggctact tgaccaccgt agtgaccgcc gcggtggtcg tgcgcctgac
     1681 ctgcaatccg ctgtggctat tggctgccgg cgccttgctc ggcatcgccg gcgtcgtgtg
     1741 atcgaggttt actttctcag cgcggggcga gcagtttttc gatctgcgcc gtgagccggc
     1801 tgtcgtcggg ttcgacgctg cttgcgaagg cggtgacctg ctgcgccgat ttgtcgacga
     1861 gatacttgtg aaaattccac ttgggcggtt gtccggtgcg cgccgcgagc tcacgataca
     1921 gcgcattggc attggcgccg accactgcgg tcttggcgaa tactggaaag gtgatgccgt
     1981 aattcacctg gcagaactgg gcgatctgct ggtttgatcc cggctcctgg ccgccgaaat
     2041 cattggctgg aaagccgaga acgacaaagc ctcgatcgcg atagcggcga taaagttttt
     2101 ccaatccgtc atattgcggc gtgtaaccgc actcgctggc gctgttgacg atcaacgcga
     2161 ctttgccgcg gaactggcac agtgatatcg gttcatccat caagttggcg aatttgtgat
     2221 caagcaatgg cggacagggc ggctcggcgg cgccgctcgg tgcggcaaac gcaaaagcca
     2281 tgagcgtggc gacgaagatt acgcagatcg atcggacgtg catgctattt ctccgtgatt
     2341 aaaatagtca tttgccgatg aatctacatg ttctggcgcc gcctgccaag ctatcgccac
     2401 agtagaaacc cgatgccgga atatcttttc attggcgctg agaagtggtt aggtctggaa
     2461 gtccgacaat ttatcactgg ataaacgccg cttcgaggag ctgccttgaa taaatccgag
     2521 accgacggtt tcgtttacgt ccgcgatgac gccgtcctgg cgaccctgac cgatcgcatc
     2581 gacagcgctg caagaatcgc cgtggatacc gaagccgata gtttgcataa ctatttcgaa
     2641 aaagtctgtt tgattcagct gtc
//
LOCUS       DASQKS010000373         7117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5228768, whole genome shotgun sequence.
ACCESSION   DASQKS010000373 DASQKS010000000
VERSION     DASQKS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5228768"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1444
                     /locus_tag="VE170_09685"
     CDS             257..1444
                     /locus_tag="VE170_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYJ15750.1"
                     /translation="MPFWNDETQRNLLHCLVTGQIIRGTDLVALRSDLLDLLGIEDAV
                     LCGSGSLALELALRSCHLDQGDEVIIPSFCCSSVVAPILAVGATPVLADVGSELNLTA
                     ETVEAARTGRTRAVLVPHLFGNPANIEQIQELGDANNIVVIDDAAQALGAFIDGRRAG
                     TFGDMGVISFGAEKVCSGLGGGVLVSRSRGVLESAREIALGHPSAAHTLARLTSTLAL
                     HRWRGWTLPFQRMLSRAHEKSPQAPPTIYRCEIMANLNAAVACSLIRSLPENLQARRA
                     RVDAYHRLLANESDIELIRHGPGSACLTQVVRIGGKGRGEDLAARIIAALGQAGYELQ
                     GSYVPIHLLTHITQCVWDRLPYSDLVWPDLIELPCEPSVPFDDLEQITAIVKSMVAGH
                     VGG"
     gene            complement(1482..2045)
                     /locus_tag="VE170_09690"
     CDS             complement(1482..2045)
                     /locus_tag="VE170_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HYJ15751.1"
                     /translation="MKKTIMAAIVVSGSLIGGGALSAQQTAPNNRNMPDANPATQVPR
                     EINPSNPGQVTPKGPGLDQKIPGQEGTIPEIIQRPGGVTPQAQTPQPQAPQPQMTVSP
                     EEIRRAQEALKTKGYDPGAASGNLHAGTQEALRKYQKANNLPVTGVLDQRTAETLGVE
                     IKNDSKPSDTKSLDNKSKEPSKSLPAR"
     gene            2341..3810
                     /locus_tag="VE170_09695"
     CDS             2341..3810
                     /locus_tag="VE170_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HYJ15752.1"
                     /translation="MKQIKFIDTTVRDGSLSLWALGMRTGAMLAIAEQMDQCGFESME
                     FFGFAGYIKYVKEHKENPWLWMGEGAKKFHRTRLRYHGGLASGFEKIPRSVRLLMIER
                     VAAHGITLTRSSDPWNDYDSAALEITALEKLGMEVIVNIIYSVSPRHSDEYFASRARQ
                     AAAIKPYRICFKDVAGLLTPERTRTLIPLIQQNIGAIPLEFHAHCNNGMAPFNYLEAM
                     KLGVETFHTGIPPLANGSGQPSIFNVARNAQALGFEPRVDLEAVTPVERHFTSIAKLE
                     GHPVGAQREYDQAQYLHQVPGGMISNLRFQLGKMGMAHRIDETLEECGRVRADFGYPI
                     MVTPLAQFVGSQAAINVITGERYKEVTDQAIQYALGHWGKEAPTVMDAGVRDKILSRP
                     RAKEWANWSVPDMTLEQVRRKVGGSNVSDEELVLRVIAGDPAVDAMLAAGAPKDYSAL
                     SQPLALLICELAKRKQYRQIYIQKNEFTLRLEQNSSNKR"
     gene            3989..4276
                     /locus_tag="VE170_09700"
     CDS             3989..4276
                     /locus_tag="VE170_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYJ15753.1"
                     /translation="MVYEMRFQTVDPARRPEYVKIYREAVQGCKSAGSTGGHILCSDD
                     DPAAVVVILLWESKEHLARWRGTDSYKSFRAAVEGRQTKKSHGGLYAAETI"
     gene            4411..6582
                     /locus_tag="VE170_09705"
     CDS             4411..6582
                     /locus_tag="VE170_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYJ15754.1"
                     /translation="MKGSKLLGFSVLAAALGGLIGLLPDRLSSQQGALVKMDSDDIGG
                     VVNGANGPEAGVWVIAETTNLPTKFAKIVVTDEQGRYLMPDLPKATYDLWVRGYGLID
                     SAKVRATPGKTLNLKAVTAPSDGAAAQYYPAIYWYSMLKIPDKKEFPGTGEKGNGMPE
                     SLKRQEQWLDVVKTNGCNTCHQLGNKATRTIPKELGEFKSSTDAWQRRIVSGQAMTAM
                     LNAIGRLDAKRATELYGEWTDRVAAGALPKSKPSRPQGVERNVVVSVWDWSNAKAYLH
                     DEISTDKRKPTVNADGPLYGSTELSTDLVPVLDPVRHRAYEIKHPVRDPDTPSAKTDP
                     LTPSPYWGTEAIWDSQSNQHNPMMDEQGRVWFTARVRPPANPDFCKKGSDHPSAKVFP
                     IASAGRHLSMYDPKSKKFTLISTCFSTHHLVFAEDADHTLWTSAGGPASGVVGWLNRK
                     MFEATGDEQKSQGWTPIVLDTNGNGKRDEWVEPNQAIDPAKDKRMAAAFYGIAVSPVD
                     GTVWGSTQGFPSAIVRLNPGPNPSETSLAEYFEVPLDTQGAYGIRGMDIDRSGVVWSS
                     LASGHLASFDRRKCKGPLNGPSATGKHCAEGWTVHTLPGPNFESLNEPAGSAESSYYT
                     WVDQFDTFGLGKDVPIATGNLNDSLIALVDNKMVNLRVPYPLGFYAKWMDGRIDNPKT
                     GWKGKGLWATTGTRTPFHMEGGKGTRPKVVKFQLRPDPLAK"
BASE COUNT         1548 a         1916 c         2084 g         1569 t
ORIGIN      
        1 gatggagaga ctgaattgag agatttaacc acaaagggca cataggtcac aaagtagggg
       61 ccgatcgcgc ccggaccatt tcggaatcgg ggaattgctg caaagaacgc aaagggcgca
      121 gggaagccag cgcaaacatt tcgatgtcac tgtggcccac caatctacac ttcgcgatcc
      181 gaaaacgatt gaacttttga accttgaacc tttgaacgat tttctccgcc gttctttccc
      241 acgcgttccg ctcgcagtgc cgttctggaa tgacgagacg cagcgaaacc tactgcactg
      301 tctggttacc ggccaaataa ttcgcggcac ggatctcgtg gcattgcgct cagatctact
      361 ggatcttctt ggcatcgagg acgccgtact ttgcggttct ggcagtttgg cgttggagct
      421 tgctttgcgc agctgtcacc tcgaccaagg cgatgaggtg attattccga gtttttgttg
      481 cagctccgtg gtggcgccga tcttggccgt gggcgcgacg ccggtgttgg ccgatgttgg
      541 tagtgaactg aatctcacgg cggaaacagt tgaagctgcg cgtacaggca gaactcgcgc
      601 ggtcctcgtg ccccatttgt tcggcaatcc ggctaatata gaacagattc aagagctggg
      661 tgacgcgaac aatatcgtcg tcatcgatga tgccgcgcag gcgttgggtg cctttatcga
      721 cggccggcgg gcgggtacct tcggcgatat gggagtaata agtttcggcg ccgaaaaagt
      781 ctgctccggt cttggcggcg gcgtgctggt ttcccgcagt cgtggagtgc tcgaaagcgc
      841 gcgggaaatt gcccttggcc atccgagcgc ggctcacacg ctcgcgcggc tgacttcaac
      901 gctggcgttg catcgttggc gcggctggac gttgccgttt caacgcatgc tttcgcgcgc
      961 ccatgaaaaa agtcctcaag ctccgccaac aatttatcga tgcgaaataa tggcgaactt
     1021 aaatgccgcg gtggcatgca gtctcattcg ttcgctgccg gaaaatcttc aagcgcgccg
     1081 tgccagagtc gacgcctatc accggctgct cgccaacgag tcggatatcg aattgattcg
     1141 ccacggcccc ggctcggcct gcttgacgca ggtcgttcgg atcgggggga aagggcgggg
     1201 cgaggacctt gcggcaagga tcatagcggc gcttggtcaa gccggttacg aattacaggg
     1261 cagttacgtg ccgattcatt tattgacgca tatcacgcag tgcgtgtggg atcgtttgcc
     1321 ttactccgat ctagtgtggc ccgatctgat cgagttgccc tgtgaaccca gcgtcccatt
     1381 tgatgatctc gaacagatta cagcgatcgt aaagtcaatg gtcgccggcc acgtcggcgg
     1441 gtagcgactt tcgccacccg ccgcgcagcg tttcaccagc gttacctcgc gggtagtgat
     1501 ttgctcggtt ctttagactt attatccaag gatttagtat cggatggctt gctatcgttt
     1561 ttgatttcta ctccgagtgt ctccgcagta cgttgatcca gaacgccggt gaccggcaaa
     1621 ttgttagctt tttgatactt acgcaaggct tcttgagtgc ccgcatgcaa attaccactg
     1681 gccgcgcccg gatcgtaacc tttcgtcttg agagcttctt gcgcccggcg aatttcttcg
     1741 ggggaaacgg tcatttgagg ttgcggcgct tgcggttggg gagtctgtgc ttgcggagtc
     1801 acaccgccgg ggcgctggat aatttccgga atggtgccct cttgaccggg aattttttgg
     1861 tccagacctg gtcctttggg ggtgacttgg ccggggttgc tgggatttat ttcacgtggc
     1921 acctgggtgg ccggattggc atcgggcata ttcctattat tgggcgcggt ttgctgcgcc
     1981 gataaagcgc cgccgccgat caacgaccct gatacgacaa ttgccgccat gatagttttc
     2041 ttcatacact gtctccttct aattttgata ttataacaat gaattctctc atgatctctt
     2101 gatgagcaac gactatgcca tcctcccggt ggcactggtg aggttaaagt cccacgggcg
     2161 ggacaatgtc aggaaaacta ttcgcaattc gtggacacct ggcactggta gaagtttgca
     2221 acgggcaaaa catgttcgac taaaaagtta cacagtgcta atcgatatac cgttaaagcg
     2281 tcgagttaga attgcgccgt ttcccacgat gctcaccgat tggagcgaag cgagagtagg
     2341 atgaagcaaa tcaaatttat cgataccacc gtgcgcgacg ggtcgttgag cttgtgggcg
     2401 ttgggcatgc gtaccggcgc gatgctggcg atcgccgaac agatggacca gtgcggtttt
     2461 gagtcgatgg agttcttcgg ctttgccggt tacatcaagt acgtcaaaga gcacaaagaa
     2521 aatccatggc tgtggatggg cgagggggcc aagaagtttc accgcacgcg gctgcgttac
     2581 cacggtggat tagcgtcggg atttgagaaa atcccacgct cggtgcgcct attgatgatc
     2641 gagcgagtgg ctgcccatgg gataactctg actcgctcgt ccgatccgtg gaacgactat
     2701 gactccgcag ccctcgagat taccgcgctg gaaaagctcg gcatggaagt gatcgtcaac
     2761 atcatctatt cggtctcgcc gcgtcacagc gacgaatact tcgcctcccg ggcccgccaa
     2821 gccgcggcga taaagccgta ccggatctgt ttcaaagacg tcgccgggct gttgacgccg
     2881 gaacgcacgc gcacgttgat tccattgatc cagcaaaata tcggtgccat tccgttggag
     2941 tttcacgctc actgcaacaa cggcatggcg ccgttcaatt atttagaagc gatgaagctc
     3001 ggcgtcgaaa cctttcacac cggcatcccg ccgctggcca acggctcagg gcagccgtcg
     3061 atcttcaatg tcgcgcgcaa tgcccaggct cttgggttcg agccgcgagt tgacttggaa
     3121 gcggtcacgc cggtggagcg acatttcact tcaatcgcca aactggaagg ccatccggtg
     3181 ggcgcgcagc gcgaatacga tcaggcgcag tatctgcatc aagtgccggg aggcatgatt
     3241 tcaaacttgc ggtttcagtt gggcaagatg ggaatggccc atcggatcga cgaaacgctg
     3301 gaggaatgcg gccgggtgcg cgctgacttc ggttatccga tcatggtgac gccgttggca
     3361 cagttcgtcg gcagccaggc ggcgatcaat gtgatcaccg gcgaacgcta caaagaggtc
     3421 acagaccagg caattcagta cgcgctgggc cactggggca aggaagcgcc gacagtgatg
     3481 gacgccgggg tcagagataa gattctcagc cggccgcgcg ccaaagagtg ggcgaactgg
     3541 tcggtgccgg acatgacgct agaacaggtg cgccgcaaag tcggcggctc gaatgtttct
     3601 gatgaagaac tggtcctgcg cgtcatcgcc ggcgatcccg ccgtcgatgc catgctcgcc
     3661 gccggcgcac cgaaagacta tagcgcactg tcccaaccgc tcgcgctgct gatctgcgag
     3721 ctcgccaagc gcaaacagta tcgccagatt tacattcaga aaaatgagtt tacgttacgc
     3781 ttggagcaga actcgtccaa taagaggtaa catcctacac ctctggaatt tacgcgattg
     3841 tgctgtttcg tccacttgag ttcatccgga gatatccatt caagccctga cttaaagtgc
     3901 cataccaaag agatcttgag ccaagtgcat taaagatttt tagcgatgtg ggctcgacgc
     3961 tatactgtaa aacgtgaagg aggagtcgat ggtttacgaa atgcgttttc agacggtgga
     4021 cccagccagg cgccccgagt acgtcaagat ttatcgcgaa gcggtccagg gctgtaaatc
     4081 ggccggctcc accggcggac atatcctgtg cagcgatgac gatccggcgg cggtggtggt
     4141 cattttgtta tgggagagca aggagcatct ggcacgctgg cgcggcacgg actcctacaa
     4201 atcctttcgc gccgcggtgg aggggcgaca aaccaagaag agccacggcg ggctttatgc
     4261 ggcggagacg atctgaagag attatttgcc ggtgacatct ggccgtgccg cgctatagat
     4321 cactgcgggc aagtttcgat tgacctgcgt tttcgatttc ggttactctc cattcgcttg
     4381 ccgtattcgt tcatttttaa ggagccattt atgaagggtt caaagttgct tggctttagc
     4441 gtgttggcgg ccgcgcttgg cggcttgatt gggctattgc cggatcgact gagcagtcag
     4501 caaggcgcgc tggtgaagat ggatagcgac gacatcggcg gcgtggtcaa cggcgcgaac
     4561 ggcccggagg ccggggtgtg ggtgatcgca gagacgacta acttgccgac caagttcgcc
     4621 aagatcgtcg tcaccgacga acaggggcgc tatctgatgc ccgatctgcc caaggcaact
     4681 tatgacctat gggttcgcgg ctatggtttg atcgattccg ccaaagtgcg cgccacgccc
     4741 ggcaagacgc tcaatctcaa agcggtgacg gcgccgagcg atggcgccgc ggcgcagtac
     4801 tacccggcga tttactggta ctcgatgcta aaaattcccg acaagaaaga atttcccggc
     4861 accggcgaga agggtaacgg tatgccggaa agtcttaagc gccaggagca gtggctggat
     4921 gtggtcaaga ccaacggttg caatacctgc catcagctcg gtaacaaagc cacgcggacg
     4981 attcccaagg aactcggtga attcaagtct tcaaccgatg catggcagcg gcgcatcgtc
     5041 tccggccagg cgatgacagc gatgctcaat gcgatcggcc ggctcgacgc caaaagggct
     5101 accgagttat atggcgagtg gaccgatcgg gttgccgctg gagcgttgcc caaatcgaaa
     5161 ccatcccgtc ctcagggagt cgagcgcaac gttgtcgtca gcgtttggga ttggagcaat
     5221 gcgaaagctt atcttcacga tgagatatcg accgacaaac gcaagccaac cgtcaatgcc
     5281 gacggtccgc tctatggctc gacggaacta agcaccgacc ttgtgccggt attggatccg
     5341 gtgcgccatc gcgcttatga gatcaaacat ccggtacgag atcccgacac gccgtcggcc
     5401 aagaccgatc cgctcacgcc gtcgccgtac tggggcacgg aggcgatctg ggacagccaa
     5461 tcgaaccagc ataatccgat gatggatgaa caaggacgag tttggttcac cgcgcgggtg
     5521 cgtccgccgg caaatccgga cttttgtaaa aaaggctcgg accatccgtc agcgaaagtt
     5581 tttccgatcg ccagcgctgg ccgccatctg tcgatgtacg atcccaagag caaaaagttc
     5641 acgctgatca gcacctgctt ttcgacccat catttggttt tcgccgagga cgccgatcat
     5701 acgctgtgga ccagtgccgg cggcccggct agcggcgtgg tcggctggtt gaaccgcaag
     5761 atgttcgaag cgaccggcga cgaacagaag tcgcaaggct ggacgccgat cgtcctcgac
     5821 accaacggca acggcaagcg cgacgaatgg gtcgagccca atcaagcgat cgatccggcc
     5881 aaggacaagc gcatggcggc ggcattctac ggcatcgcgg tcagtcccgt cgacggcacc
     5941 gtgtggggat cgacgcaggg gtttcccagc gccatcgtcc ggctcaaccc cggccccaat
     6001 ccgtccgaga cctcgctcgc ggaatatttt gaagttccgc tcgacaccca gggcgcctac
     6061 ggcatccgtg gcatggatat agatcgcagc ggcgtggtct ggtcgtcgct cgcgagcggc
     6121 catttggcga gcttcgatag gcgcaagtgc aagggtccgc tcaatggccc cagcgccacc
     6181 ggcaagcact gcgccgaagg ctggacggta cacacgctgc cgggaccgaa cttcgagagc
     6241 ttgaacgaac ccgctggcag cgccgaatcg agttattata cctgggtcga tcagttcgac
     6301 accttcggct tgggcaaaga cgtgcccatc gctaccggca acctaaacga ttcgctgatc
     6361 gcgttggttg acaacaagat ggtcaatttg cgcgtgccct atccgctcgg tttctacgcc
     6421 aaatggatgg atggccgtat cgacaatcct aagacgggct ggaaggggaa gggtctgtgg
     6481 gcgaccacgg gaacccgcac gccattccac atggaaggcg gtaaaggcac gcggcccaaa
     6541 gtcgtgaagt ttcagttgcg gcccgatccg ttggcgaagt gatttgaaat agatagggag
     6601 tcaactgggg agctatttat tgcggtagtc aatgaaaaat cgcagcccgt tgctcgcgta
     6661 tatttttcta tcattcattg actaccgtag tccgcgggac gtcccagaga gtctcgataa
     6721 aagtgttttt tgtgcttcac ctttcatgaa ttaaattctt gtcggccaat tccactgctc
     6781 tgctcgtctc cttgcttcac tccctgctta aagtggaaac cggcgtgacc gaaaacccag
     6841 agatcgaccg actcattgtc ctggactagg gtaataagtg cggccaattc cctggcttcg
     6901 tattcgtcgt tggccgaagc tttgtaatgc actattcgtc ctatgcttgg tttttgttcc
     6961 acaccaaaat tctactaaaa agtttggcaa tctgatgggg ataaactttt tttgtagaca
     7021 gtgtttaagg ccggcgatat tgtcgtaaat gcgagagtta gattaacagc gacaaccgac
     7081 cgaacccttc ggcgaaggga ttttcccaat gcagatt
//
LOCUS       DASQKS010000374         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_614990, whole genome shotgun sequence.
ACCESSION   DASQKS010000374 DASQKS010000000
VERSION     DASQKS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614990"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VE170_09710"
     CDS             <1..240
                     /locus_tag="VE170_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15755.1"
                     /translation="KLAGSLEAPFKIIAIQPLDDIAASAFSVEIQTDAKSDFKSAMIT
                     FVKDTVGGAISAWKKPESSLALSKAVLQHVLSKLP"
     gene            271..1305
                     /locus_tag="VE170_09715"
     CDS             271..1305
                     /locus_tag="VE170_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15756.1"
                     /translation="MKTPRLLLLFLWTSLWLSPLAHGQADFFAGKQIKIVVGFSAGGI
                     IDLWARLFALYLGKYIPGNPTLVVQNLPGGGSMIAANQLFNVAKPDGLTLAMLSSGLY
                     FDQLLGAKEVRYDWAKFAWIGSPVKNFEVLTMRSDTPYKTIEDIQKAAQPPRCGTTGS
                     GATGHYFPRFLDDALGAKFHLVLGYPGNREVEIAIERGEVHCYAITKEAFLREPGRSW
                     LKKGFVRVLVQGGQKRDPLFPDTPTIYELMDKHRTSEALKRFATVLLSVGTIGRPLIA
                     PPHLPAGRLATLRDAYAKMLNDGEFLGEARKREWDVEYTSGQELEAIAKQSVDQPKDV
                     VDRLRKILRD"
     gene            1606..2403
                     /locus_tag="VE170_09720"
     CDS             1606..2403
                     /locus_tag="VE170_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ15757.1"
                     /translation="MLTPLKIDNRIDAHLGVPEGIGKRPVIIHMHERYGIVQHTMDLG
                     EKYVKAGYVTVVPDYFSRFTGDRKALAEGNDRCELDDEVVLSDTDAIMAYLRTLPQAD
                     MTKVAMSGVCQTGRQPILVAAKRDYLTAAVVLYGAVYDADWKSHPRRPESIETLLQNM
                     QCPLTAVFGELDNLIHLDNIVRMTTVLAAAKKSFGVRVYPDAPHGFLNDTMPGRYRQP
                     QSDAAWNQITSFLKSVFAGEWKKDRAIWRFEADSSLDYDFTAMKRWE"
     gene            2837..3427
                     /locus_tag="VE170_09725"
     CDS             2837..3427
                     /locus_tag="VE170_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ15758.1"
                     /translation="MWFSLTNFVIGFAISVVLGVVMGVPMGWYRGVHKTFDPLLSGIY
                     ATPLIALLPLIIMMFGLGSMSKIIMTILAAVFPILINTMVGIANTDNRLITMSRAFGA
                     RDSQIFMKVSLPGSLPYIVAGMRVALGRALVYIVVAEQYGAATGLGYLSSVAAQRFQM
                     AAMFVPIVIIAGLGAGLNETLKAIERRLDKWKPQKA"
     gene            3451..4440
                     /locus_tag="VE170_09730"
     CDS             3451..4440
                     /locus_tag="VE170_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15759.1"
                     /translation="MKLIGKIFALCIFVWVLAEDSHGADALRKMTVGQAGVNPGAGLF
                     SIAVNQGFYKKHGLDVEIIKTNTTAAVQAMLANKMHMATGAGMAAFVTANLEGAPGFT
                     LVGSWVNVFPYKIMAQKGIRKVEDLKGKTGHVGAPFGTIPDTALRFALGRLKIDAEKD
                     VKLVQHSNADPASILAAMEKGEVQFGILAPPFDLIAERRGYEMLLALPGLGIPWQQNG
                     EIVPNAYLKANRDNVVRFLRATADASKFYFDSKEKTLDIMTEYLGRPRDETEYAYNQF
                     KRWTDKNHRPQVETIKTTLEAIKKNTPKAAGANAASYVDTSIVDQLIKEGYYK"
     gene            4603..5736
                     /locus_tag="VE170_09735"
     CDS             4603..5736
                     /locus_tag="VE170_09735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15760.1"
                     /translation="MKLYNFDLLAYPHVPTDAPRTPVPSSYFDPAKGAANYAEHLDEM
                     TYCEELGFDGVVFNEHHYSAYGTMPSPNLIAAALSQRTKKIKIGVLGNILPLRNHPVR
                     VAEEYAMIDCLSNGRLIAGFVRGIPPEYIWYGVNPEESRGRFEEAYNLIMTAWTEPVW
                     SHEGKFFKLHDCAIWPRPVQQPHPPIWIAARSAESVEWCVKRHLPCAQVYQTTGQIED
                     TFSYYRTKAREEGWDAKPEQFILCRHVYIDDSEQKAREFVKPAMDYFFTVFNRGFNEA
                     INKEAVDKQLQAALTSERSFSYFREGNRERFDFSKMDFDGLVKSGYLIAGNPDSVAKQ
                     IQDQMKQVGADHFMGMFHIGNLKHEKVINSLNLFTKEIMPKLR"
BASE COUNT         1321 a         1739 c         1577 g         1217 t
ORIGIN      
        1 aagttggccg gcagccttga ggcgccgttc aaaatcatcg ccatccaacc ccttgacgat
       61 atcgccgcca gcgccttctc cgtagaaatc cagaccgatg ctaaatccga cttcaaatcc
      121 gccatgatca cattcgtcaa ggacacagtt ggcggcgcca tcagcgcatg gaagaaaccg
      181 gagtcgtcgt tggccttgag caaagccgtt ttgcaacatg tcctgtccaa gctaccgtaa
      241 gtgcgtaagt acgcgagtcg aggagggatg gtgaaaaccc cgcgcctgct tctgttattt
      301 ctctggacca gcctctggct atcgccgctg gcgcacggtc aagccgattt tttcgccggc
      361 aagcagatca aaatcgtcgt gggattttcg gccggcggca ttatcgatct gtgggcgcgg
      421 ctgttcgccc tctatctcgg caaatatatt cccggcaacc cgaccctggt cgtgcagaat
      481 ctccccggcg gcggttcgat gatcgccgcc aatcaattgt tcaacgttgc caagccggat
      541 ggtctgacgc tagccatgct gtcgtcggga ctttacttcg accaattgct cggcgccaaa
      601 gaagttaggt acgactgggc caagtttgct tggattggct cgccggtgaa aaactttgaa
      661 gtgctcacca tgcgctccga cacgccctac aagaccattg aagatattca gaaagcggca
      721 cagccgccgc gttgcggcac caccggcagc ggcgccacag ggcattattt cccaagattc
      781 ctcgacgacg cgctcggcgc caagtttcat ttggtgctcg gctatcccgg caaccgcgag
      841 gtcgagatcg ccatcgaacg gggcgaagtc cactgctacg cgatcaccaa agaggccttc
      901 ctgcgcgaac cgggtcgtag ctggctgaag aaaggctttg ttcgtgttct ggtccagggc
      961 ggacaaaaac gcgatccgct cttccccgac acgccgacca tctatgaact catggataaa
     1021 catcggacat ccgaagcgct caaacgcttc gccacagtgt tgctctccgt cggcaccatc
     1081 ggccggccct tgatcgcgcc gccccatctg cccgccgggc gcttggctac actgcgcgat
     1141 gcctatgcga agatgcttaa cgacggggaa tttctcggcg aggcgcgcaa acgggagtgg
     1201 gatgtcgagt acaccagcgg ccaggaactc gaagcgatcg ccaagcaatc ggtcgaccag
     1261 ccaaaggatg tcgtcgatag actaagaaag attctgcgcg actgaccggt gccgagggag
     1321 ccacaaggac gggttgaaac cgagactacg ccggactggc tcatgaaatc gtgttgccag
     1381 tatttgcccg acttaccgca agcgctaaaa acccggagta aagagttttt gatgaatata
     1441 ccttttcgaa gtctgtaaac tccgcgtggt gcaatcctgg ttgtttcttg cgttgcgctc
     1501 cgccggctgg gctcaccgcc gaatattttt ttccccgtct agaaaatctt cgccgccaat
     1561 gttaggacga gtaagttttc accccatccg taaaaggaag cgaccatgct gacaccgcta
     1621 aaaatcgaca accgcatcga cgcccatctc ggcgttccgg aaggcatcgg caaacggccg
     1681 gtgatcattc acatgcacga acgctatggc atcgtgcagc acaccatgga tttaggcgag
     1741 aagtacgtca aggccggcta tgtcactgtc gtgcccgact atttttcccg cttcaccggc
     1801 gaccgcaagg cgctggccga aggcaacgac cgctgcgagc tcgatgacga agtggtcttg
     1861 tccgacaccg acgcgattat ggcgtatctg cgcaccctgc cccaggccga catgaccaag
     1921 gtcgccatgt cgggcgtctg ccagaccgga cgccaaccga tattagtggc agccaagcgt
     1981 gactatctta cggccgcggt ggtgctctac ggcgcggtct atgacgccga ctggaaatcc
     2041 cacccgaggc gaccggagtc aatcgaaacg ctgctgcaga acatgcagtg cccgctcacc
     2101 gcggtcttcg gcgaactcga caacctgatt catctcgaca acatcgtccg catgaccacg
     2161 gtgttggccg cggccaagaa aagcttcggc gtccgcgtct atcccgacgc gccgcacgga
     2221 ttcttgaacg acaccatgcc tggccgctac cgccagcccc agagcgacgc cgcgtggaat
     2281 cagatcacgt cgtttttaaa atcggtattc gccggcgagt ggaaaaaaga ccgggcgatc
     2341 tggcgtttcg aagctgacag ctcgctggat tacgatttta ccgcgatgaa gcgttgggag
     2401 tagcgcgcgt tgcgcgccgc tattccagat tacagattcc atattccaga ttagcaaccc
     2461 gggccatggt gacaatttgg aatctggaat ttgtaatttg gaatggggcg cacagaacaa
     2521 ccttgacaca gacataagag ccaaatagat tcagcagcac tcgagcccag aggagcgcgc
     2581 cacggcgaaa cccgaacaag aagccttcga tctagtctcc gtcgacgagt cgaatccgct
     2641 gaccaccaac cggctgtggc agcgcactcg tgagctgctc tccgattggc tcggcgtcat
     2701 cggcatcgtc gggtttttcc tcgcctggga gatttcgacc cgcctggaga tcatcaatcc
     2761 tttttatttc ccgcccttca gtaagatcat cgccaaaggc tacgagcttt tcgccaacgg
     2821 ctccatctgg gagcatatgt ggtttagcct gactaacttc gtgattggct ttgccatttc
     2881 ggtggtgctc ggcgtggtca tgggcgtgcc catgggttgg taccggggcg tgcacaaaac
     2941 cttcgacccg cttctctccg gcatttacgc cacgccgctg atcgccttgc tgccgctgat
     3001 catcatgatg ttcggcttgg gttctatgtc gaagatcatc atgacgatac tcgccgcggt
     3061 gtttccaatc ctgatcaaca ccatggtcgg catcgccaac accgacaacc gcttgatcac
     3121 catgtcgcgg gcgttcggcg cgcgcgacag ccagatcttc atgaaagtat cgttgcccgg
     3181 ttcgctgcct tacatcgtcg ccggcatgcg cgtcgctctg ggccgcgccc tggtctacat
     3241 cgtcgtcgcc gagcaatacg gcgcagccac cggtctcggc tatctaagtt cggtcgcggc
     3301 gcagcgcttc cagatggccg cgatgttcgt tcccattgtc atcatcgccg gcttgggcgc
     3361 gggcttgaac gaaactctca aggccatcga gcgccgcctc gacaagtgga aaccacaaaa
     3421 agcttgagcc agtaaactcg ggaggattgc atgaagctga tcggaaaaat cttcgcgctt
     3481 tgtatctttg tctgggtcct agccgaagac agccatggcg ccgacgccct gcgcaaaatg
     3541 accgtcggcc aggccggcgt aaacccgggt gctggactat tctccatcgc cgtcaatcaa
     3601 ggcttctaca agaagcatgg actcgacgtc gaaattatta aaaccaacac cacggccgcc
     3661 gtccaggcaa tgctggccaa caaaatgcac atggccaccg gcgctggaat ggcggctttc
     3721 gtcactgcca atctcgaagg cgcaccaggc tttaccctgg tcggctcctg ggtgaacgtg
     3781 tttccctata agatcatggc gcaaaagggc atcaggaagg tcgaagatct caaaggcaag
     3841 accggccatg tcggcgcgcc gttcggcacc attcccgaca cggcgctgcg ttttgccttg
     3901 ggccgcctga agatcgacgc ggaaaaagac gtgaagttgg tgcaacactc caacgccgat
     3961 cccgccagca ttttggccgc catggaaaaa ggcgaagtgc agttcggtat cttggcgccg
     4021 ccattcgatc tgatcgccga gcggcgcggt tatgaaatgc tcttggcatt gcctgggttg
     4081 gggattcctt ggcagcagaa cggcgagatc gtgcccaacg cctatttgaa agccaatcgc
     4141 gacaacgtcg tccgcttcct acgcgccacc gctgacgctt ccaagttcta cttcgatagc
     4201 aaggaaaaaa ccctcgacat catgaccgaa tatctcggcc ggccgcgcga cgaaaccgag
     4261 tacgcttaca atcaattcaa acgctggacc gacaagaacc accggccgca ggtggaaacg
     4321 atcaagacca ccctggaagc gatcaagaaa aacactccta aagcggccgg cgccaacgcc
     4381 gcctcctatg ttgacaccag catcgtcgac cagctgatta aagaagggta ttacaagtag
     4441 ccggcgttgc cgctgcatcg ccgcagtcct aaagccggtc cgaaacgttt taaacggctt
     4501 gaattatttg aacgttttga acggttcggc ttgtttaaga tgatttgacc catttaaacc
     4561 cgggtgttag catccgcgca gtttgttaag gagaactaat tcgtgaagct ttataatttc
     4621 gatctgctcg cctatcctca tgtgcccacc gatgcgccgc gcacgccggt gccgagcagc
     4681 tatttcgatc cggccaaagg cgcggccaac tacgccgagc atctggatga gatgacctac
     4741 tgtgaggagc taggattcga tggcgtggtt ttcaatgagc atcattacag tgcttacggc
     4801 accatgcctt cacccaactt gatcgctgcg gcattgagcc agcgaaccaa gaaaatcaaa
     4861 atcggcgtgc ttggcaacat tctaccgctg cgcaatcatc cggtgcgcgt cgccgaagaa
     4921 tacgccatga tcgactgctt gagtaacggc cgattgatcg ccggcttcgt ccgcggcata
     4981 ccgccggaat atatttggta cggggtaaat cccgaggagt cgcgcggccg cttcgaggaa
     5041 gcttacaatt tgatcatgac cgcatggacc gaaccggtgt ggagccatga aggcaagttt
     5101 ttcaaattgc acgactgcgc catctggccg cggcccgtgc agcagccgca cccgccgatt
     5161 tggatcgccg cgcgcagcgc cgagtcggtc gaatggtgtg tcaagcgcca cctgccctgc
     5221 gcccaggttt accagaccac cggccagatc gaggatactt tcagctacta tcggaccaag
     5281 gccagggaag aaggttggga cgctaaaccc gagcagttca tcctctgccg ccatgtttac
     5341 atcgacgact ccgagcaaaa agcgcgcgag ttcgtcaagc cggcgatgga ttattttttc
     5401 accgtcttca accgcggctt caatgaagcg atcaacaaag aggcggtcga caaacaactg
     5461 caagccgccc tcaccagcga gcgatctttc agctacttcc gcgagggcaa tcgcgagcgt
     5521 ttcgattttt cgaaaatgga tttcgacggc ctggtcaaat ccggttatct gatcgccggc
     5581 aacccggaca gcgtcgccaa acagatccaa gatcagatga aacaagtcgg cgccgatcac
     5641 ttcatgggca tgtttcacat cggcaacctg aagcatgaga aagtgatcaa ctcgctcaat
     5701 ctgttcacca aagagatcat gccgaagttg cgctagcacc ggtgcaacga atacgagttt
     5761 caatttatgg ttttggctcg gaataaaatg accgctaaaa cggctgctgt cctagtattt
     5821 gtcctatcgg tcctagccgc ctacgcaacc cacg
//
LOCUS       DASQKS010000375         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3562057, whole genome shotgun sequence.
ACCESSION   DASQKS010000375 DASQKS010000000
VERSION     DASQKS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3562057"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1812)
                     /locus_tag="VE170_09740"
     CDS             complement(232..1812)
                     /locus_tag="VE170_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ15761.1"
                     /translation="MASYDAIIIGGGHNGLTSAAYLAKAGKRVLVLERRHVLGGAAVT
                     EEIYPGFKYSVCSYVVSLLRPEVIRDLELPKYDLEIIPLDSTFTPMANGDRLFRSGDH
                     AQTRREIARHSPTDADAYEQYGQLMAQMAMAVKPILGMIPPEPTTWNPKDLFGLAKVG
                     NHFHDLGEKLLYELTKLMTMSSADFLDQWFDTDVLKATMSASGIIGTFMGPRSPGTAY
                     VLLHHYMGELDGAFRSWGFARGGTGAISNAIADAARHFGAEIRTQVSVDQVAIKNGRA
                     TGVILATGEEINAKVVVSSVDPKRTFLKMVDSQHLDAGFLKQVRDFKIRGSSAKVNLS
                     LDGLPDFSSMPGDGPHLSGAISISPSLDYIERAYDDAKYGDFSRQPYMDIILPSKLDP
                     SMAPPGKHVMSIFVQYAPYHLASGTWPEKREALGDTVIDTLSEYAPNIKNLILHRQVL
                     TPWDLEQEFGLTEGNIFQGELTLDQLFFLRPVAGWAKYRTPVQGLYMCGACTHPGGGV
                     MAASGRLAALEILKSARL"
     gene            1965..3158
                     /locus_tag="VE170_09745"
     CDS             1965..3158
                     /locus_tag="VE170_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HYJ15762.1"
                     /translation="MPIGTPFHPRTAALCASHNWRTWSGYFVASSYDVMHDYEYHAIR
                     NTAALIDVSPLYKYDVRGRDALKLVNRVITRDAAKCAVGQALYGCLCEDDGAVIQDGT
                     VFRLSADYFRFHLADPALRWLYLNARGMEVSIREVSEQVAALALQGPHALKILQRIVD
                     VDLAQLKFFRLRPGKILDVPVIVSRTGYTGDLGYELWFGAEHALRVWDLLIEQGSDFG
                     IKPTGILALDVARLEAGFILLEVDYIGAEKAVIESQRYSPFEIGLGWTVDLRKDHFVG
                     AKALKQCDPQRAERQIVGLDISLLDYEYLYKQAGLAPHYPLVAWRGSVPVEKDGRQVG
                     FATTGAWSPALKKYIALATLEKKYVPVGTEIDIEVTVDHRKKSAGATVVKLPFFDPPR
                     KRAVV"
BASE COUNT          686 a          917 c          994 g          715 t
ORIGIN      
        1 gccttagcga gataggcggc cgtgatcagg ccgttatggc cggcgccaat gataatcgcg
       61 tcgtagttat tcatgattga gttatttcaa tagatttttt caccacgtca aagattaaaa
      121 cccggtcgca gtggcgcgat acgcttcctg aatgggtgca tgatcgaatc tacagatact
      181 ggtataaacc ctgatcgaga taacgaacat cgctctgcca gattagcttg gctacagccg
      241 cgcgcttttt agaatttcca acgccgccag tctgcccgat gccgccatga cgccgccgcc
      301 gggatgggtg caggcgccgc acatgtaaag gccctgcacc ggcgtgcgat acttcgccca
      361 gccggccacc ggtcttagaa agaagagttg gtccagggtc agctcgccct gaaaaatatt
      421 gccttcggtg aggccgaatt cttgttccaa atcccagggc gtcagcactt ggcgatgcag
      481 gatgagattt tttatgttcg gtgcgtattc gctcaatgta tcgatgaccg tgtcgcccag
      541 ggcttcgcgt ttctcgggcc aagtgccgct ggccagatga tagggcgcat attgcacaaa
      601 gatcgacatg acatgttttc ccggcggcgc catcgatgga tcgagcttcg acggcaggat
      661 gatgtccatg tagggctggc gcgaaaagtc gccgtattta gcgtcatcgt aggcgcgctc
      721 gatgtagtcc aggctcgggc tgattgagat cgcaccgctg agatgagggc cgtccccggg
      781 catgctgctg aagtcgggca ggccatcgag cgacaggttg acctttgctg atgagccgcg
      841 gattttgaag tcgcggacct gcttcaaaaa cccggcgtcg agatgctggc tgtcaaccat
      901 ttttaaaaaa gtccgtttgg gatcgacgct ggagaccacg actttggcgt ttatctcttc
      961 gccggtggct aagatgacgc ccgtggcccg gccgttcttg atggccactt gatcgacgct
     1021 gacttgagtc ctgatctcgg cgccaaaatg gcgcgccgca tcggcgatgg cgttgctgat
     1081 cgcgccggtg ccgccgcggg caaaccccca ggatcgaaac gcgccgtcga gctcgcccat
     1141 ataatgatgc aggagcacat aggcggtccc gggcgagcgc ggtcccatga aggtgccgat
     1201 gatgccgctg gccgacatgg tcgctttgag cacgtcggtg tcgaaccatt ggtcgagaaa
     1261 atccgccgag ctcatggtca tgagtttagt cagttcgtag agcaacttct cgcctaggtc
     1321 gtgaaagtgg ttgcccacct tcgccaaacc gaatagatcc ttgggattcc acgtcgtcgg
     1381 ttcaggcggg atcatgccca ggatgggctt aaccgccatc gccatctgcg ccatcagctg
     1441 accgtattgc tcataggcgt cggcgtcggt cggcgaatgg cgcgcgatct cccgccgtgt
     1501 ctgggcgtga tcgccagagc ggaacagacg atcgccgttg gccatcggcg taaacgtact
     1561 gtcgagcggt atgatttcga gatcgtactt cggcagctcg agatcgcgga tcacttcggg
     1621 gcgcagcagg ctgacgacgt aggagcatac cgaatactta aaccccggat agatctcttc
     1681 ggtcaccgcc gcgccgccaa gcacatggcg ccgttccagg accagaactc ttttgccggc
     1741 tttggcgaga taggcggcac tggtcaggcc gttgtggccg ccgccgatga tgattgcgtc
     1801 gtatgaggcc atggtgtggt ttgattaatg aggcatattt ggtaatttcg accagcaagt
     1861 caatcagaga tgcagacaga gatgcgagat agagacagaa tgaaggcaaa tagattggga
     1921 tgggaggttg ggcggtctct atctcaaggc tctgtctagc tgccatgccc atcggaactc
     1981 cctttcaccc gcgcaccgcg gcgctttgcg ctagtcacaa ttggcgtacc tggtcaggtt
     2041 actttgtcgc cagctcctac gacgtgatgc atgactatga atatcacgcg atccgcaata
     2101 ccgccgcgct gatcgatgtc tcaccgctgt acaaatacga cgtgcgcggc agggatgcgc
     2161 tcaagctggt taaccgcgta atcacccgcg acgcggccaa atgcgcggtc gggcaggcgt
     2221 tgtacggctg tctgtgcgaa gacgacggcg cggtgattca ggacggcacg gtgtttcgcc
     2281 tgagcgcgga ttatttccgc tttcaccttg ccgatccggc cctgcgctgg ctctatctca
     2341 atgcccgtgg aatggaggtc tcgattcgcg aagtgtcgga gcaggttgcc gcgctcgccc
     2401 tgcagggacc ccatgccttg aaaattcttc agcgcatcgt cgacgtcgac ttagcccagc
     2461 ttaaattttt tcgtcttcgc ccgggcaaaa ttctcgatgt cccggtgatc gtttcgcgca
     2521 ccggctacac cggcgatctc ggttacgaac tatggtttgg cgccgaacac gcgctcaggg
     2581 tctgggatct gctgatcgag cagggcagcg atttcggcat caaaccgacc ggcatactgg
     2641 cgctcgacgt ggctcggctg gaggcgggat ttattctcct cgaagtcgac tatatcggcg
     2701 ctgaaaaggc ggtgattgaa tcgcagcgtt attcaccctt cgagattggg ctcggttgga
     2761 cggtggatct gcgcaaggac catttcgtcg gtgccaaagc gctcaagcaa tgcgatccgc
     2821 aacgcgcgga gcgtcagatc gtcggcttgg acatcagtct gctcgactat gaatatcttt
     2881 ataagcaagc cggtttggcg ccgcactatc ccttggtagc ttggcgtggc agtgtgccgg
     2941 tggagaaaga cggccgccaa gtcggctttg ccaccaccgg cgcgtggtcg ccggctttga
     3001 agaaatacat cgcgttggcc accctggaaa agaaatacgt gccggtcggc acggagatcg
     3061 atatcgaagt gacggtcgac caccggaaaa aaagcgccgg ggcgaccgtg gttaagctgc
     3121 cgttcttcga tccgccgcgc aaacgcgcgg tggtttaaag actgccggaa cgggaactcg
     3181 gaagatctcc cgcaaagtaa tccatgtacc aatcggtaca acacagcaaa tgaaaatgag
     3241 cggcgccacg ttcaaagcaa cgacggaaaa tcccccaatg gccccctttg ccaaaggggg
     3301 cgggccgaag ga
//
LOCUS       DASQKS010000376         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1456908, whole genome shotgun sequence.
ACCESSION   DASQKS010000376 DASQKS010000000
VERSION     DASQKS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456908"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VE170_09750"
     CDS             complement(<1..301)
                     /locus_tag="VE170_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYJ15763.1"
                     /translation="MTLTELVRYFLYLGAFGFGGPVALVGFMHRDLVEKRGAITEDTY
                     KLSLALAQIMPGPLAAQVAIAIGYFEAGIAGATLAGIAFVLPSFLMVVGISLVYVA"
     gene            complement(327..923)
                     /locus_tag="VE170_09755"
     CDS             complement(327..923)
                     /locus_tag="VE170_09755"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYJ15764.1"
                     /translation="MALEVERLHKRMAEIAAIDFFNTPEREAAEQVIFSLEARLQDRS
                     PAVKAARRKRQASDLQGLTWVTRKGIHVDRMASAWLVLRFIDGQARFKFVQTKGYVQA
                     RFDMYEAEFTHEGDRCTFEVLMDRAGLDDPALRAIAEIVHEVDLKDSKFSRQEALGID
                     RLIAGIAMAHRDDETRLQRASAMFDDLYAYFHRKGASV"
     gene            complement(962..1297)
                     /locus_tag="VE170_09760"
     CDS             complement(962..1297)
                     /locus_tag="VE170_09760"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HYJ15765.1"
                     /translation="MTDLLETRWLLLIHQIPPKPGYLRVKIWRRLQRLGSVAVKNSVY
                     VLPRNEQTQEDFQWVLREVVHAGGDASLCEARFIEGISDDEVEELFRSAREADYQSIS
                     REARLIAAT"
     gene            1455..2444
                     /locus_tag="VE170_09765"
     CDS             1455..2444
                     /locus_tag="VE170_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HYJ15766.1"
                     /translation="MLKVIPVEEAVGLPLAHDITEIIPGKHKGPAFRRGHIIRPEDVS
                     KLLDVGKANIYVMNLENGELHEEDAARRLAKAAAGANLKLTDPSEGRINLVATISGLL
                     KVDVDLLCRFNSLGDLMLATLPGDRYVKENTVVAGTRTIPVIVKEELIKKAEALCQGK
                     PIVTILPMPAKKVHLVVTGSEVFTGRIKDGFGPVVTKKVGDLGSTVESVKLSPDDPEV
                     IGGLIKDAKQAGADVILVSGGMSVDPDDKTPEGIRRSGATVETHGFPILPGSMFLMAY
                     LGETPVMGLSGCVLHDPFTAFDALLPRLIAGEKITRADIMAMGHGGLAKKHDH"
     gene            2481..3131
                     /locus_tag="VE170_09770"
     CDS             2481..3131
                     /locus_tag="VE170_09770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HYJ15767.1"
                     /translation="MDVLRTLKERCDPKNAALIVVDVQNDFVSPDGSAGKRGEDVSAA
                     VAMVPNLLRVIEEGRRIGLTIVYIRTTHSEWTDTPSWIYRSSQKSGLSTCREGSWGAE
                     LYDGISPLPSERVVIKHRYSAFINTDLNTVLKARNIQSVLVCGVATNVCVETTARDAY
                     MYDYYVTMIDDCSAAYEAKLHLGTLDNMRRHFGLVASSSDIIDAWRELPAKKAAGL"
     gene            complement(3222..4250)
                     /locus_tag="VE170_09775"
     CDS             complement(3222..4250)
                     /locus_tag="VE170_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYJ15768.1"
                     /translation="MAEAKVNRLNLTEKDYDGAPSTMCKGCGHDAISASVQRAYYESS
                     IDPRKVVKLSGIGCSSKTPAYFLSQSFGFNAVHGRMSAVATGANLANHTLLPIGVSGD
                     GDTSAIGLGNFCHMMRRNVNITYIIENNGVYGLTKGQFSATADVGSVMKGGKVNDIPA
                     IDLAELAITLGATYVARSFSGDRKQLTPLVQGALAHRGSAILDVISPCVTFNNHEGST
                     KSLKYVKGHLDPIHDLDFVPPYENIEADYDEGTDHEVAMHDGSRIILHKLSKHYDPTS
                     KSNAIQAIHGAIAEGKFLTGLIYYNPNRKEFAEELNLCDTPLSELGQDLLQPPAEALE
                     RINAEMMK"
     gene            complement(4269..>5096)
                     /locus_tag="VE170_09780"
     CDS             complement(4269..>5096)
                     /locus_tag="VE170_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYJ15769.1"
                     /translation="QADISLMYGASHGDTRHIILIPHDMNSCFDLARQSFNYADRFQT
                     PVFVAMDLDLGMNMWSSEPLKLTQEPFDIGKRLNAQDLDEWTKAGKKFRRYFDQDGDG
                     IPYRTVPGNENRLASYFTRGSGHDADAKYSEDEHDYKYILDRLRQKFDTARKFVPKPI
                     IEKEKGVKTGIICYGSSYEPVREARDRLKARGLKTNHMLIRALPLTSEVTDFLAEHDT
                     IYLVEQNRDAQMAAIIKDENPLLGAKITSILVYNGLPVSAGEVVKQIFDAAQTAKTA"
BASE COUNT         1133 a         1337 c         1422 g         1204 t
ORIGIN      
        1 aggcgacata aaccagcgag atgccaacga ccatgagaaa cgaaggcaat acgaaggcga
       61 ttcccgctaa ggtcgcaccg gcgatcccag cctcgaaata gccgatggct atggcgactt
      121 gcgcggccaa aggtcccggc atgatctggg cgagcgccag ggacagtttg taggtatctt
      181 cggtgatggc tcctcgtttc tcgaccaggt cgcggtgcat gaagccgact aatgccaccg
      241 gaccgccgaa accaaaggcg ccgagataga gaaagtaacg aaccagttcg gtcagggtca
      301 tcaagtctct ctttacggat tgagcattac accgatgcac ctttgcggtg aaaatacgcg
      361 tacaaatcgt cgaacattgc cgacgcgcgt tgtaaacgcg tttcgtcatc tctgtgcgcc
      421 atcgctatac ctgcaatcaa gcgatcgatg ccgagagctt cctggcgcga aaacttgcta
      481 tccttgagat caacctcgtg aacaatttcc gcgatcgctc gaagagccgg atcgtccagc
      541 cccgcgcgat ccatcagcac ttcgaaggtg cagcgatctc cctcgtgggt aaattccgcc
      601 tcgtacatat cgaaacgagc ttgtacgtag ccttttgtct gaacaaactt aaaccgtgct
      661 tgcccatcga tgaatcggag caccagccag gcgctcgcca ttctgtcgac atgaatgcct
      721 ttgcgcgtga cccacgttaa gccttggaga tcgctggcct gccgttttct ccgtgcggct
      781 ttgaccgccg gagaccggtc ttgcaggcgc gcttcgagag aaaagatcac ctgctccgca
      841 gcttctcgct ccggcgtgtt gaagaaatca atcgctgcaa tttccgccat gcgcttatgc
      901 agccgctcca cttcaagagc caactgtgtt gtgcgttcat catcgctctt gcgcttcggg
      961 gtcaggtggc agctatcagc cgggcttccc tggatatgct ttggtaatca gcttctcggg
     1021 ccgaacgaaa cagttcctcc acttcatcgt cggagatacc ttcaatgaaa cgcgcctcac
     1081 aaagcgaggc gtccccgcct gcgtgaacaa cttcacgcag tacccactgg aaatcctctt
     1141 gggtctgttc attcctgggt aaaacataga cagagttttt caccgcgacc gagccgagac
     1201 gctgcaagcg ccgccaaatc ttcactctca gatagcctgg ttttggcggt atttgatgaa
     1261 tcagtaatag ccagcgtgtt tcaagcaaat ctgtcatgat attataactg tatcgtaaat
     1321 atataataac tgtatcatcg gatcaacgct tcggtcttcg tgaaatccgc atgatcgctg
     1381 ctgaaaaacc atttttagtg gtatagaatt cagcatcgca agccaaaggt ttattgaacg
     1441 gagagagtaa agtcatgctc aaagtcatcc ctgttgaaga agcggtagga cttcccctcg
     1501 cccacgacat taccgagatc atccctggta agcataaagg accggccttt cgacgcggcc
     1561 atatcatccg ccccgaagac gtctcgaaac tcttagatgt cggcaaggcg aatatttacg
     1621 tcatgaatct agagaacggt gagctgcacg aagaagacgc ggcgcgccgg ctggccaaag
     1681 cggcggcggg ggctaattta aaattaaccg atccgagcga aggtagaatt aatctggtcg
     1741 cgaccatttc cggtttgctc aaggtcgatg tcgatctgct ttgccgtttc aattccctcg
     1801 gtgacctcat gctcgcgaca ttgcccggcg atcgctacgt caaggaaaac accgtcgtcg
     1861 ccggcacgcg aacaatcccc gtcatcgtca aagaagaatt aataaagaaa gccgaggcac
     1921 tgtgtcaggg caagccgatt gtgacgattc tacccatgcc agccaagaaa gttcatttag
     1981 tggtcaccgg cagtgaagtt ttcaccggaa gaatcaaaga cggcttcggc ccggtggtga
     2041 ctaaaaaagt cggcgatttg ggctcgacgg ttgaatcggt gaaactatct ccggacgatc
     2101 cggaggtgat cggcggttta ataaaggacg ccaagcaagc gggtgcggac gtgattttag
     2161 tcagcggtgg catgtctgtc gatcctgatg acaagacccc ggagggaatt cgtcgcagcg
     2221 gcgcgaccgt ggaaacccat ggttttccaa ttttacccgg ctcaatgttc ttgatggctt
     2281 atctcggcga gacgccggtg atgggattgt ccggctgcgt gctgcacgat ccgtttaccg
     2341 ccttcgatgc tttgttgccg cgcttgatcg ccggcgagaa aatcacccgc gccgatatca
     2401 tggccatggg ccatggtggc ttagcgaaga aacacgatca ctgataacag tggtcgggat
     2461 cagttagaaa ggagttctct gtggatgtct tgcgaacctt gaaagagcgt tgtgacccga
     2521 aaaatgctgc actgatcgtc gtcgacgtgc agaatgattt tgtcagccct gacggcagcg
     2581 ccggcaaacg cggcgaagat gtcagcgcgg ccgtggccat ggttcccaac cttttgcgtg
     2641 tcattgaaga ggggcggcgc attgggttga cgatcgttta cattcgcacc acccatagcg
     2701 aatggaccga tacgccgtcg tggatctatc gctcatcgca aaagagcggg ctcagcactt
     2761 gccgggaagg cagttggggt gcggaactct atgacggcat ttcgcccttg ccgtccgagc
     2821 gggtcgtcat caagcaccgc tacagcgcgt tcatcaatac cgatctcaac accgtgctca
     2881 aggcgcgtaa tatccaaagc gttctcgtct gtggtgttgc caccaacgtg tgcgtcgaaa
     2941 ccacagcgcg ggacgcctac atgtacgatt attatgtgac catgatcgat gactgttccg
     3001 ctgcttatga agcgaagtta catctcggaa cactcgataa tatgcgccgt cacttcggcc
     3061 tggtggcctc gtcaagtgat atcatcgacg cttggcgcga actgcccgcc aaaaaagctg
     3121 ccgggctata atacttccgc gtggcattat ttatcggcaa taaaaaaagc ccggtagggc
     3181 atctaccggg cttttttttc taattgagaa tacttgttca gttacttcat catttcggcg
     3241 ttgatacgct ccaaggcttc ggcgggcggc tgcaacaagt cctgtcccaa ttccgatagc
     3301 ggcgtgtcgc aaagattaag ttcttcggca aattccttgc ggttgggatt gtagtagatc
     3361 aaaccggtga gaaacttgcc ttcggcgata gcgccatgga tcgcttgaat cgcattggac
     3421 ttgctggtcg gatcgtaatg cttgctgagt ttgtggagga ttatgcgcga gccgtcgtgc
     3481 atcgcaactt catggtccgt gccctcgtcg tagtcggctt cgatattttc atagggcggc
     3541 acaaagtcca gatcgtggat cggatcgaga tggcctttga cgtatttcag actcttcgtc
     3601 gatccctcgt ggttattaaa cgtcacgcag gggctgatga cgtcgaggat cgcgctgcct
     3661 ctgtgtgcca gggctccctg cactagcggc gtgagttgct tgcggtcgcc ggaaaaactg
     3721 cgggcgacgt aggttgcgcc gagggtgatc gccaactcgg ccaaatcgat ggcgggaata
     3781 tcgtttactt taccgccctt catcaccgaa ccgacatcgg cggtggcgga gaactggcct
     3841 ttggtcaagc catagacgcc gttgttctcg atgatgtaag tgatgttcac attgcgccgc
     3901 atcatatggc aaaaattgcc gagcccgatg gcagaggtat cgccgtcgcc ggagacgccg
     3961 attggcagca aggtatgatt cgctaagtta gcgccggtgg ccaccgccga catgcggcca
     4021 tgcacggcgt tgaagccgaa ggattggctt aaaaaatagg ccggagtttt cgacgaacag
     4081 ccgatgccgc tgagtttgac caccttgcgc gggtcgatgc tgctttcata gtaagcgcgc
     4141 tggactgagg ctgagattgc gtcatggccg caacctttgc acatggtcga cggcgcgccg
     4201 tcataatctt tttccgtaag gtttaggcga tttacttttg cttctgccat tgtcgtgtcc
     4261 ttatatcgtt aagcggtttt cgcggtttga gcggcatcga agatctgttt gacgacttcg
     4321 ccggcgctga ccgggaggcc gttgtagacg agaatgctgg tgatcttggc accgaggagc
     4381 ggattctcgt ctttaatgat tgcggccatt tgagcgtcgc ggttctgctc gaccagataa
     4441 atcgtatcgt gctcggccag gaagtccgtc acttcgcttg tcagcggaag cgcgcggatc
     4501 aacatatgat tggtctttaa gccgcgtgct ttcaaccggt cgcgggcttc gcgcaccggc
     4561 tcgtagctgg agccgtagca aatgatgccg gtcttaacgc ctttttcttt ttcaatgatc
     4621 ggtttgggga caaatttgcg cgcggtgtcg aacttttgac gcaggcggtc gaggatgtac
     4681 ttgtagtcat gctcgtcctc ggaatacttg gcgtcggcgt cgtggccgga accgcgggtg
     4741 aagtaagagg ccaggcgatt ctcgttaccc ggcacagtac gatagggaat gccgtcgccg
     4801 tcttggtcga aatagcggcg gaattttttg ccggctttgg tccattcatc caaatcctga
     4861 gcgttcagtc gcttgccgat gtcgaagggt tcctgggtca gcttgagcgg ttccgacgac
     4921 cacatattca tgcccaggtc gagatccatg gcgacgaaga ccggagtctg gaagcggtcg
     4981 gcgtaattga aactctggcg cgcgagatcg aagcaggagt tcatgtcatg gggaatcaga
     5041 ataatatgac gggtatcacc atgagaggcg ccatacatca gtgaaatatc ggcctg
//
LOCUS       DASQKS010000377         6827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4814114, whole genome shotgun sequence.
ACCESSION   DASQKS010000377 DASQKS010000000
VERSION     DASQKS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4814114"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VE170_09785"
     CDS             <1..377
                     /locus_tag="VE170_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15770.1"
                     /translation="LGGIDDRVLGRPDWNLLLAKHGPPDLLMAHNPDLFYEAEKLAVP
                     LTLSGHTHGGQIRFPNGPAIIRQSRYCLDEGVYCFGKSQLVVSRGLGSVFLPIRWGAD
                     AEAVMIEIVSPAQPRPASQLNS"
     gene            complement(491..1471)
                     /locus_tag="VE170_09790"
     CDS             complement(491..1471)
                     /locus_tag="VE170_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15771.1"
                     /translation="MKNIAVAALLFGVYLLVSGSAAGQTLKTGYISKDLNYLPFFVAQ
                     KKGFHAKEGIAVDLVSIGRSDIQLQALVAGELHFANINADNIIIWNERTGGNLKIAAG
                     SSNAAPYLLIGAKNIKRIEDLKGQRIGVNSLSGGATSILVAYLRTKGLIHPRDFSMSV
                     IAGGTPARLSALESGAVAGAVLVLPFSDIAVDRGFTKLGDTTEVISSYQFNNININPA
                     WAEKNRPSVVKFIKSHIQALRWLHDHPADAAEFLTKEFGLQAVYARRGIDYYTKTKVY
                     PYNGDVTLAGLKVNIDVQVQDGIIKGAVPAPEKYIDLSYLRQAQKELGIQ"
     gene            complement(1503..2462)
                     /locus_tag="VE170_09795"
     CDS             complement(1503..2462)
                     /locus_tag="VE170_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HYJ15772.1"
                     /translation="MTEKIIYLNGAFVAESEAKVSVLDSGFNAGDGVYDVTRTFAHRP
                     FRLRDHTARLFRSLRYTRIDCGLTLEQMEAATLEVLERNRPALGKDDEVAIWQVVSRG
                     VRTSRGNHALGKATVAIYCISIAFEEFARDYLDGVKLVIPSTRRIPPQCLEPKAKINN
                     KMNHNMAIFEARQVDPHAIPLMLDLDGNISESNAHNFFLVLDGKLCTPSNKNVLDGIT
                     KQALYELAANLEIEVVDGNFTPYDAYNAEEAFLASTSPTILPVQSINGVRPTKETPGP
                     ITKRLIQGWNKMVELDIVEQALAHLEPAERDRLVKEWRSKSAS"
     gene            complement(2617..4029)
                     /locus_tag="VE170_09800"
     CDS             complement(2617..4029)
                     /locus_tag="VE170_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15773.1"
                     /translation="MASRINYLLLATALLGTFFSGTATRIVSISMPTVAQSLGTDLLG
                     ISWALLSYQLSNIGLSVVFGRISDIWGREKMFALGFLVFAVSSLLCGFSQTVLHLIAA
                     RFIQGVGGAMLQSSSRALAAESVSEDLAGRAQGFMTTAHHVGFILGPGIGGVMIDYFS
                     WRWSFFLLAPVGFGGTLLALANMRRGRTAIGQHSSGTVDYLGAALLFAITSTLVFIFD
                     RRTQEAVGNSLRLVLLVTFFLSLAGFLVHESRAKNPFVNLQLFKIRRFSFSVISLLIV
                     AMCYSLTGFLLPFYLQDILHMSPTKIGVLFMAPSILTVALAPLSGYLADRLGPRIPAT
                     VGVVFMVISLGIGGLLRVDSHWLLPTLLVVVGAITNGVFNPANSTAMIAMMPKEHRGF
                     ASAINHVTFGFGSVFGVALGGLCMSLAFEQYTGIKTTSLTTDNAQGFVAALNLTFVAA
                     IVLTLVAIATSAMRGAEKPR"
     gene            complement(4394..5635)
                     /locus_tag="VE170_09805"
     CDS             complement(4394..5635)
                     /locus_tag="VE170_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ15774.1"
                     /translation="MLYNKERLIALMDRFDLVGVVAATPENIYYLSGHASWSQNGYRY
                     GGSQVYVVYPRDPAHKPALLIPGSDVGYASIDDVRIDEKYIYGRPRNPHITDMSKLSA
                     VEQRMVKLAGSDSKGLTAEKALAQLIKDKGMDKGRIGLDHFAIPITQYEKIRANLPRT
                     KLLPASTFFRYVRMIKTADEIQRLREAAEVNEKAINSMLRGCRPRVTEDELASIYKGE
                     IAKAGGQVYWMHMAVTRGGNFPPIKDNVLQKGDIFRVDMGCSINGYHADVCKSGCVGA
                     EPTAEHRKRYDAIQAGVLQSIEALKPGVRPQELYETMIAGVRANGLPDYSNFFLGHTI
                     GLEAREFPFLLGPAEELDDPFLPNTTNIPVEPGMTMNLEASNHEAGWGTVQVEYTCAV
                     TATGHEHLIFPEQKLLTLPLE"
     gene            complement(5748..>6827)
                     /locus_tag="VE170_09810"
     CDS             complement(5748..>6827)
                     /locus_tag="VE170_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ15775.1"
                     /translation="KRPLIYVGRGALWSGATDALIQVAESYALPVMATLPAKGAMPED
                     HPLCLGLGGYPRAVYSTGQSRKFVEEADLVIALGCSFRQHATSSWLPKPAHTKLVQVD
                     IDAAELHKNYPADLVVLSDIQLFLRAWLSQGKEKLAVYRKGMAADVAGEIKQLKNKWL
                     DSWSPRLTSDEVPMNPYRACWDLTHQLDAKKTILLHDAGVTRAYVSHHYEALNPLGFI
                     GFGGTSAMGWSTPAAMGAKLGNPEKTVVNFTGDGSFGMTGMEIETAARNDIRTLTVIL
                     NNESLGASRETLHERFKGHEIGISLRGDYAGVARALGAFGERISKPQELQPALQRALA
                     HPGPAVLEIAVKPLEPRPWRSNTGV"
BASE COUNT         1469 a         2010 c         1937 g         1411 t
ORIGIN      
        1 tcttgggcgg gatcgacgat agggtcctgg gccggccgga ctggaatctg ctgctcgcga
       61 aacacggccc gcccgatctg ttgatggcgc acaaccccga tctgttttac gaagcggaaa
      121 aacttgccgt cccgctcacc ctttccggcc acacccatgg cggacaaatc cgtttcccca
      181 acggcccggc gatcatccgc caaagccgct attgcttaga tgagggggtt tattgcttcg
      241 gcaaatctca actcgtcgtg tcgcgcggct tgggcagtgt cttcctgccg atccgctggg
      301 gtgcagacgc cgaggcggtg atgattgaaa tcgtatcgcc agcccagccg cggccggctt
      361 cgcagttaaa ttcgtaatca attcctgctt ggcggtcgca ccaggctaag taagattgtt
      421 gaaacggccc atcggtcaaa tcttgaccat cattagttgg atccactgcg aaactcctat
      481 cttaggcctg ctattgaatc cccagctctt tctgcgcctg gcgcaggtag ctcagatcga
      541 tatacttttc cggcgccggc accgcgccct tgatgatgcc gtcttgcacc tgcacgtcga
      601 tattgacctt caagccggcc agagtcacgt cgccgttgta ggggtaaact ttggtcttgg
      661 tatagtagtc gatgccgcgg cgggcgtaga ccgcttgcaa accgaactct ttggtcaaaa
      721 attcggcggc gtcggccgga tgatcgtgca accagcgcag cgcttgaata tgcgacttga
      781 taaatttcac cacgctcggg cggttcttct cggcccatgc cggattgata ttgatattat
      841 tgaactggta gctcgaaatc acctcggtgg tatcgccgag cttggtaaat cctctgtcga
      901 cggcaatatc agaaaacggc aagaccaaca cggcgcccgc caccgcgccg ctctcgaggg
      961 cgctcaatcg tgccggtgtc ccaccggcga tcaccgacat ggaaaagtcg cgcggatgaa
     1021 tcaagccctt ggtgcgtagg taggcgacca gaattgacgt cgcgccgccg ctcaggctat
     1081 tgacgccaat ccgttggcct ttcagatcct cgatgcgctt gatgttcttg gcgccaatca
     1141 ggagataggg cgcggcattg ctcgatcccg ccgctatttt caagttgcca cccgtgcgct
     1201 cgttccaaat aatgatgttg tcggcgttga tgttggcaaa gtgaagttca cccgccacta
     1261 gggcttgcaa ctgtatgtcg ctgcggccga tgctgaccaa atccaccgcg atgccttctt
     1321 tggcgtgaaa gccttttttc tgggcgacga aaaacggcag gtaattgaga tccttcgaga
     1381 tgtaaccggt tttgagagtt tgccccgctg ccgaacctga gaccaagaga tagactccga
     1441 acagcagcgc agctacggca atatttttca tacgtgctcg gttttccttc gcttgagcgc
     1501 agtcacgaag cgctcttgga gcgccactct tttaccaagc gatcgcgctc agccggctcc
     1561 aagtgtgcca acgcttgctc gactatgtcc agctcgacca ttttgttcca gccctgaatc
     1621 aaacgtttgg tgatcggccc gggagtttct ttcgtcggcc taacgccgtt gatactttgc
     1681 accggcagga tcgtcgggct ggtgctggcg agaaacgcct cttcggcgtt gtaggcgtca
     1741 tagggagtaa aattgccgtc gaccacttcg atttccaagt tcgccgcgag ctcgtacaaa
     1801 gcctgcttgg tgatgccgtc gagcacgttc ttgttcgacg gcgtgcacag cttgccgtca
     1861 agaacgagaa aaaagttgtg cgcgttggac tcgctgatgt tgccgtcgag atcgagcatc
     1921 agcgggatcg cgtggggatc gacctggcgc gcttcgaaga tcgccatgtt gtgattcatt
     1981 ttattgttga tcttagcctt gggctccaga cactgcggcg gtatgcgccg ggtggacgga
     2041 atgaccagct tcacgccgtc gagataatcc cgggcgaatt cctcgaaggc gatggaaata
     2101 cagtaaatcg ccaccgtcgc cttgccgagc gcatgattgc cgcgcgatgt gcgcacgcct
     2161 cgactgacca cttgccagat cgccacttcg tcgtctttgc caagcgctgg ccggttgcgt
     2221 tcgagcactt cgagggtcgc cgcttccatc tgctccaagg tcaagccgca gtcgatgcgc
     2281 gtgtaacgga gcgagcgaaa caaacgcgcc gtatggtcgc gcaggcgaaa gggccgatgc
     2341 gcaaatgtcc gagtgacatc gtaaaccccg tcgccggcgt tgaagccgga gtcgagcacc
     2401 gaaactttcg cttcgctctc cgccacgaaa gcgccgttga gataaatgat cttctcggtc
     2461 atgacccgtc tcctttctgt cctgcccggc aagccgcccg gattatttcg agaaccaagc
     2521 tcgattacaa cccgagcgcg ctttcgtcgg catgattccg gagcagcgtt tcagtatcca
     2581 atttaaaacc ggccatttgg cttgccatgg cgcatgttag cgcggcttct cggcgccgcg
     2641 catggccgaa gtcgcgatcg ccaccagcgt taggacaatt gccgcgacga aggtcagatt
     2701 gagcgcggcg acaaagccct gggcattgtc ggtcgtcagg ctggtcgtct ttatgccggt
     2761 gtactgctcg aaggccaagg acatgcacag cccgcccaaa gccacgccga acacgctgcc
     2821 aaacccgaaa gttacatggt tgatcgccga ggcaaagccg cggtgctcct tcggcatcat
     2881 ggcgatcatc gccgtcgaat tggcgggatt aaaaacaccg ttggtgatgg cgcccacgac
     2941 aaccagcaga gtcggcaaaa gccaatggga gtccacccgt agcaacccgc caatgccgag
     3001 ggagataacc atgaacacaa cccccaccgt ggcgggaatg cgcgggccga gacggtcggc
     3061 gagatagccg ctcagcggcg ccaacgccac ggtgagaatc gacggcgcca tgaacaggac
     3121 accgatcttg gtcggcgaca tatgcaaaat atcctgcaag taaaacggca ggagaaatcc
     3181 ggtcagcgaa tagcacatgg cgacgatcag caagctgatg acgctaaaac tgaagcggcg
     3241 aatcttgaac agctgtaaat tgacgaaggg gtttttcgcg cgcgactcat ggacgagaaa
     3301 gccggctagg gacaagaaga aagtgacgag cagaaccagc ctaagcgaat ttcccaccgc
     3361 ctcctgggtg cgccggtcga agatgaacac caatgtgctg gtgatcgcga acagcagcgc
     3421 cgctcccagg taatcgacgg tgccgctgct atgttgcccg atcgccgtgc gtccgcgtcg
     3481 catattagcc aacgccaaca gcgtgccgcc aaagccgacc ggcgcaagca ggaaaaagct
     3541 ccagcgccag ctgaaataat cgatcatcac cccgccgata ccgggaccca agataaagcc
     3601 gacatggtgc gccgtggtca tgaacccttg cgccctgccg gcgagatcct cgctcaccga
     3661 ctccgccgcc aacgcccggc tcgacgactg caacatcgcc ccgccgacgc cttgaatgaa
     3721 acgggcagcg atcaaatgca aaaccgtctg ggaaaacccg cagagcaatg aactaacggc
     3781 aaagaccaag aaacccaggg cgaacatctt ctcccgcccc caaatatccg aaatccggcc
     3841 gaacaccacc gataggccga tgttagaaag ctgataggac agcaacgccc aagagatccc
     3901 gagcaagtcc gtgcccaagc tttgcgccac cgtcggcatt gaaatcgaca cgatgcgcgt
     3961 cgccgtgccg gaaaagaatg tgcccaatag cgcggtggcg agaagcagat aattgatgcg
     4021 cgatgccatg tcggtgcgat tctcacaaac gaatttctga gtaatagcgc cgaaccaccg
     4081 atggagcaaa ccttagcggg gatttagcga cagatcgcgg agactcgatc cactacaaaa
     4141 actctagtgc ggcatcatta cactcacagc gtgaagttat tgcactgaga acagaggcaa
     4201 tcgcacatca gaagaatggg ttcggcgtcg agacatcgca tcattactcg cgccggcatg
     4261 aaggccggtt agattcacgc tcacgacggc gcgagaacga aaacgagagg tttcaccgcg
     4321 aatttaaggt tctgggtctc caagagaaca tttcattcac gaaatacgac gtctgaaaag
     4381 tcgcgtcgaa ttgtcactcc aacggcagtg tcaataactt ttgctccggg aaaattagat
     4441 gctcatggcc ggttgcggtc acggcgcagg tatattcgac ttggacggtg ccccagccgg
     4501 cttcgtggtt cgaggcttcc aagttcatgg tcatgcccgg ttcgacgggg atgttggtcg
     4561 tgttgggcaa aaacggatcg tcgagctctt cggcgggacc gagcaggaat ggaaactcgc
     4621 gcgcttctag tccaatcgta tggccgagga agaagttcga atagtccggc aggccgttgg
     4681 cgcgcacgcc ggcgatcatg gtttcataga gctcttgcgg ccggactccc ggcttcagcg
     4741 cctcgattga ttgcaacacg cctgcttgga tagcgtcata acgcttacgg tgttccgccg
     4801 tcggttcggc gccgacgcag cccgacttgc acacgtcggc gtgatagccg ttgatcgaac
     4861 agcccatgtc gacgcggaag atatcgccct tctgcagcac gttgtctttg atcggaggaa
     4921 aattgccgcc gcgcgtcacc gccatgtgca tccaataaac ctggccgccg gccttggcga
     4981 tctcgccttt gtagatactc gccagctcgt cctcggtcac gcgcggacgg cagccgcgca
     5041 acatcgagtt aatcgccttt tcattgacct cggcggcctc tctgagccgc tgaatctcat
     5101 cggcggtctt gatcatgcgc acgtagcgaa aaaatgtcga tgccggcagc agctttgtcc
     5161 gcggcagatt ggcgcggatc ttttcgtact gcgtgattgg aatcgcgaag tgatccaggc
     5221 cgatgcgccc tttgtccatg cctttgtctt tgatcagttg cgccagcgct ttttctgcgg
     5281 tcaacccttt cgaatcgctg ccggcgagct tcaccatgcg ctgctcgacg gcagaaagct
     5341 tggacatgtc ggtgatatgc ggatttctcg gccggccgta aatatacttc tcgtcgattc
     5401 gcacgtcatc gatagaagca taaccgacat cgctgccggg aatcagcagc gccggtttgt
     5461 gcgccgggtc gcgcggataa accacataca cttgcgagcc gccgtagcga taaccgttct
     5521 gactccagga cgcatggccg ctcaggtaat aaatattctc cggcgtcgcc gcgaccacgc
     5581 ccacgagatc gaaacgatcc attagggcga tgagtctttc cttattgtac aacatgggaa
     5641 ccatcctcct attcgcaatc caaattggag tactggagtg atggagtact gggaaatccc
     5701 atcccaacca ccactccatt attccaatac tccatcactc catttcgtca tactccagtg
     5761 tttgatctcc acggccgcgg ttcgagcggc tttacggcga tctctagcac cgcggggccg
     5821 ggatgagcca aagcgcgttg cagcgccggt tggagctcct gcggtttcga gatgcgctcg
     5881 ccgaaggcgc ccagcgcccg cgccacgccg gcgtagtcgc cgcgcagcga gatgccgatc
     5941 tcgtgccctt tgaagcgctc gtgcagggtc tcgcggctgg cgccgagact ttcgttgttg
     6001 aggatcacgg tcaaggtgcg gatgtcattg cgcgcggcgg tttcgatctc catgccggtc
     6061 atgccgaagg aaccgtcgcc ggtgaagttc actacggtct tttcgggatt gcccaacttc
     6121 gcacccatcg cggccggcgt cgaccagccc atggcgctgg tgccgccgaa accgatgaag
     6181 cccaagggat tcaacgcttc gtaatgatgc gacacatacg cgcgcgtcac gccggcatcg
     6241 tgcagcaaaa tggttttctt ggcatcgagc tgatgggtca agtcccagca ggcgcgatag
     6301 ggattcatcg gcacttcgtc ggaagtcagc cgtggactcc aggaatcgag ccacttgttc
     6361 ttcagttgtt taatctcgcc ggcgacatcg gcggccatgc ctttgcgata gacggcgagc
     6421 ttttctttac cctgcgacaa ccaggcgcgc agaaacagtt gaatgtccga gagcacgacc
     6481 agatcggcgg gataattttt gtgcagttcg gcagcgtcga tatcgacctg gaccagtttc
     6541 gtatgggccg gcttgggcag ccagctggaa gtcgcatgct ggcggaaact gcagccgagc
     6601 gcaatgacga gatcggcctc ttcgacgaat ttacgcgact gcccggtgct gtaaaccgcg
     6661 cgcggataac cgccgaggcc gagacagagg ggatgatcct ccggcatcgc gcccttcgcc
     6721 ggcaaggttg ccatcaccgg taaggcatag ctctcggcaa cttgaatcaa ggcatcggtg
     6781 gcgcccgacc atagcgcgcc gcggccgacg tagatcaacg gccgctt
//
LOCUS       DASQKS010000378         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1458592, whole genome shotgun sequence.
ACCESSION   DASQKS010000378 DASQKS010000000
VERSION     DASQKS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458592"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VE170_09815"
     CDS             complement(<1..388)
                     /locus_tag="VE170_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15776.1"
                     /translation="MSGSVSSHGGRIDSFRQGLRELGYVEGKNIFIEYRYDEGKRERF
                     DDLAAEMVRLKPEVIYARSTGFASAGKKATSTIPIVATGGDLVGSGLAASLAQPGGNV
                     TGSTNMSPDVSGKRLELLKEAVPRAKR"
     gene            complement(702..1049)
                     /locus_tag="VE170_09820"
     CDS             complement(702..1049)
                     /locus_tag="VE170_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYJ15777.1"
                     /translation="MIHPGEVYMADFAPAGPHPVIVVSREELNRGRYVLAVVCTSARF
                     AVRSKLSNCVPFHAGDFGFTVDCVAQCENILSIDQAQLDLAAGPMGTLDEAALQEVIK
                     SIGYVMNSDCKPL"
     gene            complement(1046..1264)
                     /locus_tag="VE170_09825"
     CDS             complement(1046..1264)
                     /locus_tag="VE170_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15778.1"
                     /translation="MSIQLTPQQQQALDSEKDLPPRIVDPRTNTSYILVPAVDYESIR
                     ELLDDERRQQAVHAVALRNAAGRIDDIP"
     gene            complement(1743..2729)
                     /locus_tag="VE170_09830"
     CDS             complement(1743..2729)
                     /locus_tag="VE170_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HYJ15779.1"
                     /translation="MKAVWYDRQGPAREVLNYGDMPTPEAGRGEVRIKLQASGVNPSD
                     TYRRSGTAGPMEYARVIANSDGAGIVDQIGVGAVRFKIGDRVWLYNGQRNGRAFGTAA
                     EFIALDENLVTPLPDNLSFAAGATLGVPGMTAWVCLFQGGSIDGQTVLVTGGAGAVGH
                     YGIQLAKWAGARVITTVSTSQKGEIARAAGADLVINYKREDVAARVLSFSPKGVDRIV
                     EVDFGGNIATTEKILARFSTIAVYASKGNLTPQVTVRMLSGKNATIYFMQLPLTPMVL
                     QRKAQAGMAEWLTGARSIHMIAAQFPLNQTVEAHLAVEAGTKLGTVIVDCAR"
     gene            complement(2820..>3635)
                     /locus_tag="VE170_09835"
     CDS             complement(2820..>3635)
                     /locus_tag="VE170_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15780.1"
                     /translation="PTVAGLAGETFGRLKDCELEIACVRAYNDWLIDEWASASERFIP
                     QCLVPIWPVEAAIAEIQRAVGRGHRGVILPSLPMHLRDVPHISGPEYDPLWSVCEELA
                     VPVCLHAGASAELQSPLPPNMKPALAAAVDAATKPVSSVFVISLYSFSRVLLRHPKLR
                     LVLAESALSWGMLYLEWADHQFEHDGLAREGYDLTPLQMFKRQCFFNAWYDPVAPFTD
                     YIGADPILWSANFPLANSTWPRTRETIDHCFKGVSAEAREKVLWKNAAELYRL"
BASE COUNT          786 a         1103 c         1006 g          740 t
ORIGIN      
        1 cgcgcttggc tctgggaaca gcttccttta agagttccag tcgctttccg cttacatccg
       61 gcgacatgtt ggtagatcca gtaacgttgc ctcccggctg cgccaagctg gcggcgagtc
      121 cactgcccac caggtcgcct ccggttgcaa cgatcggaat cgtgcttgtt gccttttttc
      181 ctgcgctagc gaatccggta ctcctggcat agattacctc gggttttaat cgaaccatct
      241 ccgccgcgag gtcgtcaaat cgctcacgct taccctcgtc gtatctgtac tcaatgaaaa
      301 tgttctttcc ctcgacataa cccagttcac gcaggccttg acgaaaagaa tcgatccgac
      361 cgccatgact tgagaccgaa ccgctcacta acatcccaat ccgtgcgatt tttatctgct
      421 gctgtgcgtt ggctgacgct caaacaacaa ccagtgtcat tgcaatcagg attgatgcca
      481 caggactttt cttcattacg caggcttcct atcacgccca cggtagttgt gaaacgacct
      541 atttctggca gaagtaaaaa cgatttgtcg agggaagtaa ggcgagatcg gtctgacacg
      601 cgacgggatc gtaccaggcg ttgaagaagc attggcgcga gagataataa cgggtcggga
      661 aactattttc tccaaatgga gcgggctcag aaggcccgac ttcacagtgg tttacaatcc
      721 gagttcatga cgtagccaat cgatttgatg acctcctgaa gggccgcctc atccaatgtt
      781 cccatcggac ctgcggctag gtcgagctgc gcctgatcga tggagagaat attctcacac
      841 tgcgccacac agtctacggt gaagccaaag tctcctgcgt ggaacggcac acaattcgat
      901 aacttactgc gaacggcgaa acgggccgac gtacaaacaa cagctaacac atagcgtcct
      961 cggttcagtt cctcccgcga taccacgatt actgggtgtg gacctgcggg ggcaaaatcg
     1021 gccatgtaga cttcaccggg atggatcatg gaatatcgtc aatgcgacca gcagcgttgc
     1081 gcaaagcgac cgcatgaaca gcctgctgac gacgctcgtc atccagcagt tcccgaatgg
     1141 attcatagtc caccgcggga actaggatat agctcgtgtt ggtacgggga tcgacgatac
     1201 gcggcggaag atctttttcc gaatccaagg cttgttgttg ctggggagtt agttggatac
     1261 tcatagcgac ctccttccga aacattgtac ttatctaaaa gaccacactc aatcggagag
     1321 cgcaaaggag cgcgggcttt ctagcccgcg agaatatggc tgacaagaag gtctatcctc
     1381 ccttaaaact cgatcggcct ctacactcga tacagctccg cagcatttct cgacaacact
     1441 ctttcgcgcg cctccgccga aactcccttg aaacaatgat cgatagtccc gtaggttctg
     1501 ttagcatagc gtaacccgac gctaaacccg acattcgccg cctcaggcta gcacggtcaa
     1561 acaccaacga cttacatggc gaatgcggtg gagaaactca tgtcttcaag aacggactgg
     1621 cagtgtccca tcgcgtcttg gtgaatgcga ctctgccatg cggacagcgg tgccagcgac
     1681 catcttcgga accgtcgctg cgctgcgcta acccgaccca tgaaacttga atccgaccgc
     1741 gctcaacgtg cgcagtcgac gataacggtg ccgagtttgg ttccagcttc caccgcaagg
     1801 tgcgcctcga cggtttgatt cagcggaaac tgcgccgcga tcatatgaat gctgcgggcg
     1861 ccggtgagcc actcggccat tccggcctgc gcctttcgct gcaacaccat cggcgtcagc
     1921 ggaagctgca tgaaataaat agtggcgttc ttgccgctca gcatgcggac cgtcacctgg
     1981 ggtgtgagat tgcccttgct cgcatagacc gcaatggtgg aaaaccgcgc aaggattttt
     2041 tccgtggtgg cgatgttgcc gccgaagtcg acttcgacga tgcggtccac gccctttgga
     2101 ctgaagctga gcacccgcgc agcgacatcc tcccgcttgt aattgatcac aagatccgcg
     2161 ccggcggcgc gagcgatttc gcccttctgc gacgtgctca cggtcgtgat gacgcgcgcg
     2221 ccggcccatt ttgccagctg aatcccgtag tgcccgaccg ctcctgcgcc gccggtgacc
     2281 agcaccgttt gtccgtcgat agaacccccc tgaaacagac agacccatgc ggtcatgccg
     2341 ggcacgccga gcgttgcgcc agccgcgaac gacaggttgt ccggcagcgg cgtaaccagg
     2401 ttttcgtcga gcgcgatgaa ttcagctgcg gtgccaaatg cgcggccgtt gcgctggccg
     2461 ttgtagagcc agacgcggtc gccgatcttg aaacgcacgg cgccaacgcc aatctggtcg
     2521 acgatgccgg cgccgtcgct gttggcgatc acgcgcgcat actccatcgg gccggcggtg
     2581 cccgagcgtc gataggtgtc ggacggattg acacccgagg cctgaagctt gatgcggacc
     2641 tcgccgcggc cagcttccgg cgtgggcata tcaccgtagt ttaagacttc gcgcgccgga
     2701 ccctgcctgt cgtaccagac tgccttcata tcgtgttccg atgaagtaac ccctacgatc
     2761 taaaaatttc gcgtcggggc atcgatcacc aaggattccg aaatgccgct ctccggcttc
     2821 tatagccgat acagctccgc ggcatttttc cacaacacct tctcgcgcgc ttccgccgat
     2881 actcccttga aacaatgatc gatggtttcc cgggtgcgcg gccaggtcga gtttgccagc
     2941 gggaaattgg ccgaccagag gatcgggtcg gcgccgatgt aatcggtgaa gggcgcgacg
     3001 ggatcgtacc aggcgttaaa aaagcattgg cgtttgaaca tctgcaacgg cgtgagatcg
     3061 taaccctcgc gggcgaggcc gtcatgctcg aactggtgat cggcccattc cagatagagc
     3121 atgccccaac tgagcgcgct ctcggcaagc accaggcgca gtttcgggtg gcgcagcaaa
     3181 actcgcgaaa aagaataaag tgaaattacg aatacgctcg acaccggctt ggtcgccgcg
     3241 tccaccgccg cggccagcgc cggtttcata ttcggcggca gcggcgactg caattccgcc
     3301 gatgcgccgg cgtggaggca aaccggcacg gccaattctt cgcataccga ccagagcgga
     3361 tcatattcag gaccactgat gtggggaacg tcacgcaaat gcatcggcaa ggacggcaag
     3421 atcacgccgc gatggccgcg gccgaccgcg cgttggatct cggcgatcgc cgcttccacc
     3481 ggccagatcg gcaccaggca ctggggaatg aagcgctcgc tggcgctcgc ccactcgtcg
     3541 atcaaccaat cgttgtacgc gcggacgcag gcgatctcca actcgcagtc cttcaggcgg
     3601 ccgaaggtct cgccagccaa accggccacc gtcgg
//
LOCUS       DASQKS010000379         5990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2310356, whole genome shotgun sequence.
ACCESSION   DASQKS010000379 DASQKS010000000
VERSION     DASQKS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310356"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1361)
                     /locus_tag="VE170_09840"
     CDS             complement(159..1361)
                     /locus_tag="VE170_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15781.1"
                     /translation="MDNYDNVLVVDADGHVYEGNIDLRPRIPEKWRSQAPINLKDNEG
                     NGRMLLEGRLWSASQGLGPGVSGPMTDKARGFREGMVDPLARLKDMDAEGIDAAVLFG
                     TQIALTVNGLMSKELSAVLCHACNDWLMEYCSAEPKRLLGVGLIPCQDPQAAVKELEY
                     LKKAGAVTAMLPTNVYGLNNMGDKMFDPIYECAQAIGMTLSVHPQTGHDGVPGVSGVM
                     GAGSERFRKYVYVHMTAFPFELMIAMMHMIGEGVFDRFPKLKVGFMEGGAGWLPFWAE
                     RFDEHIEKLAPQMPALKRRPSEIIKSKQIVLTCESEETGLDRVFAANGEDTVLYASDY
                     CHWDCHFPYSVKDVVDSKDLSFAQKEKLLGKNAVEFFELKNLPKPNALKIAHASWANG
                     DQPKAAMA"
     gene            1499..2722
                     /gene="cofG"
                     /locus_tag="VE170_09845"
     CDS             1499..2722
                     /gene="cofG"
                     /locus_tag="VE170_09845"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HYJ15782.1"
                     /translation="METILQNCRRGKLLSRAEALSLVDAEGEGLQALLATAAELRDRH
                     KGRTVTFSPKIFVPLTNLCRDFCGYCTFRKAPDEPGAKTMTLDEVLRSVRQGKALGCT
                     EVLFSLGDKPEAIYPEMKQFLTERGHQRTLDYLVEACRVVLEDTGLLPHSNPGVMGRG
                     DLQRLKEFNPSMGLMLESVSGRLMAAGAAHDNAPDKKPAVRLRTLELAGELRIPFTTG
                     ILIGIGENWPERIDSLLAIGRLHERYGHIQEVIVQNFRAKPTIPMHSHPDATRNDMVK
                     TIALARLILGGGMNIQAPPNLTPDGYELYLHAGINDWGGVSPLTPDFINPEAPWPALR
                     MLQEKSAQAGFELKARLPIYPEYIRQGDKYLPAQVRAYIEPLCDADGLVKNGGPLGDA
                     PLRQAFIAPSLPGLA"
     gene            2722..3648
                     /gene="cofD"
                     /locus_tag="VE170_09850"
     CDS             2722..3648
                     /gene="cofD"
                     /locus_tag="VE170_09850"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HYJ15783.1"
                     /translation="MLVVLTGGTGGAKLIEGLAAETDPAELTIICNTGDDAVFHGLHV
                     SPDIDTITYTLAGLSDSKKGWGIHGDTFATLEQLRRLGNDAWFNLGDKDLATHITRTQ
                     WLNEGLKLTEVTERIGRQLGVRSHLLPMTNERVETRVLTPQGELSFQEFFVKERWERE
                     VTSVRFVGAEQAHPAPAVLDAIHAAQVIFVCPSNPITSIGPILAVPGIRAALKQTGAP
                     VVGVSPIIGAAAISGPAHRLMVASGFPPSAAGVAGCYADFLDTLLIADEDRSSGPAIT
                     AFHITPVCTDIRMGDIAAKRRLARELLALVKK"
     gene            3667..4356
                     /gene="cofC"
                     /locus_tag="VE170_09855"
     CDS             3667..4356
                     /gene="cofC"
                     /locus_tag="VE170_09855"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HYJ15784.1"
                     /translation="MHVAALMPVKGFHNAKQRLSPLLGIGARELLAETMFRDVLRQVR
                     LARGLAGTFVVTGDDKVAAIAAAAGAAVIRENAENGETSAVDFARLELKQSGCQAVLI
                     IPADMPLMRAEDIDAVLAQVPADAAAPYALLVPSHDKLGTNALLLAPPDVIQLRFGHD
                     SFTFHMGQVAGRGMPALFFENENIALDIDEPKDLERFLAYGYQGGESTRVARELLAAD
                     VTPTRQGSGGI"
     gene            4353..5105
                     /gene="cofE"
                     /locus_tag="VE170_09860"
     CDS             4353..5105
                     /gene="cofE"
                     /locus_tag="VE170_09860"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HYJ15785.1"
                     /translation="MKRVELVGLDGIAEIHTGESLGQLICDACERQQFPLADGDVLVV
                     AQKIVSKAEGQMIALATVQPSARAQELARELGKEAELIEVILGESRKIIRTGGRALIV
                     ETHHGFICANAGVDQSNVGIKHVALLPKDSDRSARGIRAEIQRLSGKRVGVVVSDSFG
                     RAWRVGTVDVAVGVAGIDPIKDERGLKDRHGYELKAAVAAVADELASAAELVMGKRDG
                     VPVVIVRGYDIKINEEGSVQELLRPEAEDLFR"
     gene            5119..5781
                     /gene="npdG"
                     /locus_tag="VE170_09865"
     CDS             5119..5781
                     /gene="npdG"
                     /locus_tag="VE170_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HYJ15786.1"
                     /translation="MDNFTVAIVGGTGNLGSALALRLGAPGVKIIIGSRAAEKAQLAV
                     ETLKPQLRAGEMTGMTNQAAVKDANFVVIAVPYEGQAQMVQDLKGQVAGKIIIDTVVP
                     LNKIKPFVPPAGSALQEAQQILGDEAPVIGALHNISAVDLGDVDSPLGDVLVCGDNAE
                     AKAKVMEIIERIGATAYDAGPASNAYVIEGLTGVIIALNRKYKSKHGSIKITGIGKQP
                     GH"
BASE COUNT         1208 a         1684 c         1839 g         1259 t
ORIGIN      
        1 acacgcttga agtctgattt tcttgccatc tccgattttt tgaccctttc gcttgcctcg
       61 aagcgcgagg ctaaaaagga ggcgacgaca aggtaaacgc aaacggcggt gcgcgcacgc
      121 ttggtgctga gaatccaatg gttccatggc aaaactcctt acgccatggc ggctttaggt
      181 tgatcgccgt tggcccagct ggcatgagcg atcttgagcg cattgggctt gggcagattt
      241 ttcagctcga agaattctac ggcgtttttg cccagcagtt tttctttctg ggcaaagctc
      301 aggtctttgc tgtcgaccac gtctttcacc gagtagggga agtggcaatc ccagtggcag
      361 taatccgaag catagagcac ggtgtcctcg ccgttggcgg cgaatacgcg gtcgagaccg
      421 gtttcttcgg attcgcaggt gaggacgatt tgcttgctct tgatgatctc gctgggccgc
      481 cgttttaacg cgggcatttg cggcgcgagc ttttcgatgt gttcgtcgaa acgttctgcc
      541 cagaacggca gccatccggc gccgccctcc atgaagccga ccttgagctt cgggaagcga
      601 tcgaacacgc cttcaccgat catgtgcatc atggcaatca tcagttcaaa gggaaaggcg
      661 gtcatatgga cgtagacgta ttttctaaag cgctcgctgc cggcgcccat gacgccggaa
      721 acgccaggca caccgtcgtg gccggtttgc ggatggacgc tcagcgtcat gccgatggct
      781 tgagcgcatt catagatcgg gtcgaacatc ttgtcgccca tgttgttcaa accgtaaacg
      841 ttggtcggca gcatggccgt gaccgccccg gcttttttca aatattccaa ttccttcacc
      901 gcggcttgcg gatcttggca gggaatcagg ccgacgccga gcaatcgttt cggctcggcc
      961 gagcagtatt ccatcaacca atcgttacag gcgtggcaga gcacggcgga cagctctttg
     1021 ctcatcaagc cgttgaccgt gagcgcgatc tgggtgccga ataataccgc ggcgtcgatg
     1081 ccttccgcgt ccatatcttt gagacgcgcc agcggatcga ccatgccttc gcgaaagccg
     1141 cgcgccttgt cggtcatcgg accggagacg cccggaccga gaccctggga ggcggaccac
     1201 aggcgtcctt cgagcaacat gcgcccgtta ccttcgttgt ctttcaggtt gatcggcgct
     1261 tgcgagcgcc acttttccgg tatgcgcggc cgcaagtcga tattaccttc ataaacatgg
     1321 ccgtcggcgt cgacgaccag aacgttatcg taattatcca tggcgattcc tcccagcgac
     1381 tctcgctaga acgagaccta ccacagcgaa agtctctagg gcaaccagaa cgggacagct
     1441 cggcgagctg cgatactggt ttgaataccg gcggtcaatt ggatattcaa acacacagat
     1501 ggagacgatt ctgcaaaact gccgccgcgg caagctgctg tcccgtgccg aggcgctatc
     1561 tttggttgac gccgagggag aaggtttgca ggcgctgctg gcgaccgcag ctgagttgcg
     1621 cgaccggcac aaggggcgca ccgttacttt ttcaccgaag atcttcgtgc cgctcaccaa
     1681 cctgtgccgg gatttttgcg gctattgcac ctttcgcaaa gcgccggatg agccgggcgc
     1741 gaaaaccatg acgttggacg aggtgttacg ctcggtgcgc caaggtaaag cgctgggctg
     1801 cacggaagtg ctgttcagcc tcggcgacaa gcccgaggcg atctacccgg agatgaaaca
     1861 atttttgacc gaacgcggcc atcaacgcac cctcgattac ctagtcgaag cctgccgcgt
     1921 ggtgctggaa gataccgggc tactgccgca ctcgaatcca ggcgtcatgg gacgcggcga
     1981 tttgcagcgg ctgaaggagt tcaacccgag catgggtttg atgctcgaaa gcgtcagcgg
     2041 acgcttaatg gccgctggtg ccgcccacga taatgccccg gacaaaaagc cggcggtgcg
     2101 gctgcgcacg ttggagctgg ccggcgagct gcgcattcca ttcaccacgg ggatcctcat
     2161 cggtatcggc gagaattggc cggaacggat cgattctctt ttggcgatcg gccggctgca
     2221 tgagcgctat ggtcacattc aagaagtaat cgtgcagaac tttcgcgcca agccgacgat
     2281 tcctatgcac agccaccccg acgcgactcg gaacgacatg gtcaagacca tcgccctggc
     2341 gcgcttgatt ctcggcggtg ggatgaatat tcaagcgccg cccaatctga cgccggacgg
     2401 ttatgaactt tatctacacg cgggtatcaa tgattggggc ggcgtgtcgc cgctgacgcc
     2461 tgacttcatc aatcccgagg cgccctggcc ggcgctgcgc atgcttcagg agaaaagtgc
     2521 gcaggcggga ttcgagctga aagcgcggct gccgatctac cccgagtata ttcgccaggg
     2581 tgataaatat ttgccggcgc aggtgcgggc ttacattgag ccgctgtgcg atgccgatgg
     2641 cttggtcaag aacggcggac cgctgggcga cgctccattg cggcaagctt tcattgcgcc
     2701 atctctgccc gggcttgcct gatgctggtc gttctcaccg gcggcaccgg cggcgccaag
     2761 ttgatcgagg gtctggccgc tgaaaccgat ccagcggaat tgacgattat ctgtaatacc
     2821 ggtgacgacg cggtgtttca cgggctgcat gtctcgcccg atatcgacac gatcacctat
     2881 acgctggcgg gtttgagcga cagtaaaaag ggctggggca tccatggcga cactttcgcc
     2941 acgctggagc agctgcgccg gctcggcaat gacgcatggt tcaatctcgg cgataaagat
     3001 ttggcgacgc atatcacgcg cacccagtgg ctcaacgaag ggctgaagct caccgaagtg
     3061 accgagcgga ttggccggca gttaggggtg cggtcacatc tcttaccgat gaccaacgag
     3121 cgggtcgaga cccgcgtgct gacaccgcag ggggagcttt cctttcagga attcttcgtc
     3181 aaagagcgct gggagcgtga agtcacctcg gtccgcttcg ttggcgcgga acaagcccat
     3241 ccggcgccgg cggtacttga tgccattcat gccgcgcagg tgatcttcgt ttgtccgagc
     3301 aatccaatta ccagcatcgg tccgatactc gcggtcccag gaattcgcgc agcgctcaaa
     3361 caaaccggcg cgccggttgt cggcgttagc ccaatcatcg gtgccgcagc gatttccggt
     3421 ccggcgcata gactcatggt tgctagcggt ttcccaccat cggctgccgg ggttgccggt
     3481 tgttacgctg actttctcga tacgttgctt atcgccgatg aggatcgctc ttccgggccg
     3541 gcgattacgg cgttccacat cacgccggtc tgcaccgaca ttcgcatggg tgatatcgcc
     3601 gcaaaaaggc gcttggcgcg cgaactattg gccttggtca aaaaatagtc taaaagcttt
     3661 ctttccatgc acgtcgccgc gctaatgccc gtgaaaggtt ttcacaatgc caagcaacgt
     3721 ttgagcccgc tgctgggtat tggtgcgcgt gaacttttgg cggaaaccat gttccgcgat
     3781 gtcctgcgcc aggtgcggct cgcccgtggg ctggccggaa catttgtcgt taccggcgac
     3841 gataaggttg ccgctattgc ggcggcggcg ggtgcggccg tgatccgcga gaacgccgag
     3901 aatggcgaga ccagcgccgt cgattttgcc cgtctcgagt taaagcagag cggttgccaa
     3961 gcggtgctga tcattcccgc cgatatgccg ttgatgcgcg ccgaagatat cgacgcggtg
     4021 ctggcgcagg ttccagccga tgcggctgcg ccctacgcgt tattggtgcc gtcccatgac
     4081 aagctcggca ccaacgcgct gttgttggcg ccgccggatg tgattcaact gcgctttggc
     4141 catgatagtt tcacttttca tatgggccag gtagccgggc gagggatgcc ggcgcttttt
     4201 ttcgagaacg agaacattgc tctcgatatc gatgaaccga aggatctcga acggtttctt
     4261 gcctacggtt atcagggcgg cgagtcgacc cgtgttgcgc gggagttgct cgctgccgac
     4321 gttacaccga cgcgccaagg ctcagggggc atatgaaaag ggtggaactt gtcggcttgg
     4381 acgggatcgc cgagattcac accggcgaat ccctcgggca gcttatttgc gatgcctgcg
     4441 agcggcagca gtttcccctc gccgacggcg atgttctcgt ggtggcgcaa aagatcgttt
     4501 ccaaggccga aggccaaatg attgcgctgg caacggtcca gccttcggcg cgggcgcagg
     4561 agctggcccg cgaactaggg aaagaagccg aactgattga agtcattctc ggcgagagtc
     4621 gcaaaatcat tcgtaccggc ggacgggcac tgatcgtcga aacgcaccat ggttttatct
     4681 gcgccaatgc cggcgtggat caatcaaatg tcggcatcaa gcatgttgcc ctgttgccca
     4741 aggactcgga ccgttcggcg cgcgggattc gtgccgagat tcagcgcctg agcggcaagc
     4801 gggtcggcgt tgtggtcagc gatagctttg gccgcgcctg gcgagtcggc accgtggatg
     4861 tggcggttgg ggtcgccggc atcgacccga tcaaggatga gcgcggtctg aaagatcgcc
     4921 atggttatga actaaaagcg gcggtggcgg cggtggccga tgaactggca tcggccgccg
     4981 agttggtgat gggcaaacgc gacggggtgc cggtggtgat cgtgcgcggc tatgacataa
     5041 aaattaatga ggaaggatct gtgcaggagc tcttgcgccc cgaggcggaa gatctttttc
     5101 gctaggttgg aaaataatat ggacaacttc actgtagcta tcgtcggcgg cacgggaaac
     5161 ttgggcagcg ccttagcgct gcgtctaggc gcgccggggg ttaaaattat catcggctcg
     5221 cgcgctgccg agaaagctca gctggcggtc gagactttga aaccccagct gcgcgccggc
     5281 gagatgaccg gtatgaccaa ccaagccgcg gtcaaggacg ctaactttgt ggtgatcgcg
     5341 gtgccttatg aaggccaggc gcaaatggtg caggatttga aaggccaggt agcggggaag
     5401 attatcatcg atacggttgt gccgctgaat aaaatcaagc ctttcgtgcc tcccgccgga
     5461 tcggcattgc aggaggcgca gcaaattctc ggcgacgaag cgccggtaat cggcgcgctg
     5521 cataatatct ccgccgttga tctcggcgat gttgattcgc ccttgggcga cgtgctggtc
     5581 tgcggcgaca atgccgaagc gaaagcgaaa gtgatggaga tcatcgagcg catcggcgcc
     5641 acggcctatg acgccgggcc ggccagtaat gcttatgtca tcgaagggtt gaccggcgta
     5701 atcatcgccc tcaaccgcaa gtacaaatcc aagcacggca gcatcaagat caccggcatc
     5761 ggcaagcaac cgggacattg agcaagtatg atggcgggtt tcgcgcctta tcgtgcgcat
     5821 gtcccacttt gaatcgcgcg cagcctaagc agtaataaag gatttacgct ctccagcagc
     5881 ttggcgcaga agcgcggttt taacgctccc ggcgaatcct tgggtccctc tgggacccgg
     5941 aatcttcgat agaaaaccat aacaatccgt tagttaagtc attgccgcca
//
LOCUS       DASQKS010000380         5205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3568906, whole genome shotgun sequence.
ACCESSION   DASQKS010000380 DASQKS010000000
VERSION     DASQKS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568906"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2385
                     /locus_tag="VE170_09870"
     CDS             <1..2385
                     /locus_tag="VE170_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ15787.1"
                     /translation="PQMTSQQSRQEERVFAYDVSRSVPPSMRPWMANMTKQLVPGNSD
                     RIYTFAGAVKQSATLEQALGGDASASPPDKTNLENLLTELLALPAMPRSVFLFTDGWE
                     TDGNVERLLPAAAAAGIKIYPMLPAERPAIANVAVTRVIAPSQGMSGESVNLKVSLEN
                     QNDRPVDGVLTLTRNGQPFKSDRIKLRPGSQSVSYQANLVEGSLTAYRASFAAADPTA
                     DIFTPDNYALVWINVRTKAKVLIVNGPGGTGRYLDEIARRQGFDIVTHGAEGAPAPAG
                     HKIVIFNNVPRERFSAAYLASVERHVAGGGSFIMLGADASFAQNSYRQTAIEKILPVE
                     PKEPPKQPEKNRAVVLVIDKSGSMRDDNRILFAKEAAKGVARQLKDIDLLGIVGFDDS
                     PFVVVYLETVARLRSVIDNQIDRLRPGGQTYFLPALLEARRQLERSNASRKHVILISD
                     GVTRGSQGELVDLVGLMKNDSKITVSAVAVSNEADVRVMKRVAQYGGGLFHHVIDPST
                     LPQIVLDSLQDKPRDEPKDTRPLIPVQSGDSELLGDFGVKNYPPVLGYMETELKRGAH
                     ADIVVPRDDRRAPLLASWRFGKGKSVAFTSDLEGRWTRNWVPWGGLQGLWGRIFEWLS
                     PSEQNLIPLHEARVNFTGGQSILDLTVYEESGANSQFRFSLTGKSGKTEGVLSKLAPG
                     HFQAVLPAGASGDYRIDLTEERGGQRIAYPPVAYTLAYDRSAEVPRPEFNYRLLARLA
                     EASGGEINPRSLDGLRRESLTKNFRPVRHPLLIMAFVLLLVEIALRRIVFRESD"
     gene            2592..3914
                     /locus_tag="VE170_09875"
     CDS             2592..3914
                     /locus_tag="VE170_09875"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYJ15788.1"
                     /translation="MIVAAIAVAPLNGEASKQLPAEKQAVRGGKQAPKPASSTPVKRL
                     TKVQAHRVLLRQPSSSPTNLSDKDKYELVNYRVRPGETLSDVLTRHKVSKAETLLWAR
                     TMTRTVGSETLLSGKETNLFFGKSLMRGRGPVARGPLKAVEIDQNESFNLILEKGIRG
                     IYVQSREKPYDVEVKAVSAAIENSLFDDGRRAGVHPALLSQLADLFTWDIDLEKDIRK
                     GDHFKILYEQRSRKGQDTNTGLRILAAELHSAGQKHTAVYFERQKGVGNYYNQEGRGL
                     ARAFLRFPLEFSAITSPFSESRFHPLLRTNLPHTGVDFAAQRGTPVRAVGDGIIAEAG
                     WNGGYGKAIDIKHDATYSSRYAHLDGFAEGIQAGVAVTKGQLIGYVGSTGRSTGPHLH
                     FELYKDEKYINPLSIDFPAEDHLEPALHRLFENQMRTYMAELKSSPQS"
     gene            3992..4465
                     /locus_tag="VE170_09880"
     CDS             3992..4465
                     /locus_tag="VE170_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HYJ15789.1"
                     /translation="MSRLRHKFTLALATGAGVGYSRFFPGTMGSLVAVPISLTLNRLA
                     ETTPMFALIVLVGLTLGAIWLSTIAAEILQAKDPQIIVADEIVGFMIANFLAPRRWAP
                     LLLVFLLFRIFDIVKVYPAARLEKLPGGLGIVLDDVLAGVYAFVIVQTLLYWRWL"
     gene            4462..>5205
                     /locus_tag="VE170_09885"
     CDS             4462..>5205
                     /locus_tag="VE170_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HYJ15790.1"
                     /translation="MIEKAVILSTGDELTTGKVVDTNSAFIADRLFALGIRVAAILKV
                     GDDRDKLLWALSQAQELGDIVIGTGGLGPTTDDLSTELIADFVGLKTKLDEGVAAALK
                     ARFAKRGLPFTPNNLKQAFFPEGAAIIPNPNGTAPGFHIDLGDGKKLIWLSGVPHEMS
                     AMLLESVLPWVVEQTGGGPTTIHEATFKIHGISEGKLDDLVKPLDLDNFAKLSFRAHF
                     PDLTLRLTMSGGAARTEHFLNLRGQIKTIL"
BASE COUNT         1147 a         1533 c         1450 g         1075 t
ORIGIN      
        1 ccgcagatga cgagccagca atcccgccag gaagaacggg tcttcgccta cgacgtctcg
       61 cgtagcgtgc cgccgagcat gcgcccgtgg atggcaaaca tgaccaagca actggtgcct
      121 ggcaatagcg accgcatcta tactttcgcc ggcgcggtca agcaaagcgc cactcttgaa
      181 caggctcttg gcggtgacgc cagcgccagc ccgccggaca agaccaatct ggagaatctt
      241 ttaactgagc tcctcgccct gccggcgatg ccgcgttcgg tctttttgtt cacggatggt
      301 tgggaaaccg acggcaacgt cgaacggctg cttcccgccg ccgccgcggc ggggattaag
      361 atatatccga tgttaccggc agagcggccg gctatcgcca atgtcgccgt aacccgagtg
      421 atcgcgccca gtcagggcat gagcggcgaa tcggtaaact taaaagtcag cttggaaaac
      481 cagaacgacc gcccggtgga cggcgtgctg accttaacca gaaatggtca accgttcaaa
      541 agcgaccgga tcaagttacg tccaggcagc cagagcgtta gctaccaggc caatctcgtc
      601 gaggggtcgc tcaccgccta tcgagcaagt ttcgcagctg ccgatcccac tgccgatatt
      661 ttcaccccgg acaattatgc actggtgtgg attaacgtgc ggacaaaagc caaggtcttg
      721 atcgttaacg gtcccggcgg cactggtcgc tacttggacg agatcgcccg ccgccaaggt
      781 ttcgatatcg tgacccacgg cgccgaaggc gcgccggcgc cggcagggca caaaatcgtg
      841 atattcaata acgtgccgcg cgagcgcttt tccgcggctt acctcgccag tgtcgagcgc
      901 catgtcgccg gcggcggctc tttcatcatg ctcggcgccg acgccagctt tgcccagaac
      961 agttatcgtc aaaccgcaat cgaaaagatt cttccggtgg aaccgaaaga gccgcccaaa
     1021 cagccggaaa agaaccgcgc cgtggtgttg gtcatagaca aatcaggaag catgcgcgac
     1081 gacaaccgaa ttctattcgc caaggaagcc gccaaagggg tggcacggca gctcaaggat
     1141 atagatctat taggcatcgt cggattcgac gatagtcctt tcgtcgtggt ctatctggaa
     1201 accgtggcgc ggttgcggag cgttatcgac aaccaaatcg accgtctcag gccgggtggt
     1261 cagacttatt tcctaccggc gttgctggaa gctcggcgcc aactcgaacg gtcaaatgcc
     1321 tcgcgcaagc atgtcatcct aatcagcgac ggcgtaaccc gcggcagtca gggcgagctg
     1381 gtggatcttg ttggcttgat gaaaaacgac agcaaaatta ccgtctccgc cgtcgcggtc
     1441 agcaacgaag cggatgtcag agtcatgaag cgggtagccc aatacggcgg cggcctgttc
     1501 caccacgtca tcgacccgtc gactctaccg cagatcgtcc ttgattcgct ccaagacaag
     1561 cctcgcgatg aaccaaaaga cactcgcccg ctgattccgg tgcagtccgg cgactccgaa
     1621 ctgttgggcg attttggcgt gaaaaattat ccgccagtgc ttggctatat ggaaaccgaa
     1681 cttaagcggg gcgcgcacgc cgacatagtc gtaccgcgcg atgaccgccg cgcgccgttg
     1741 ctcgcctcat ggcgttttgg caaaggcaaa tcggtcgcct tcactagcga tttggaggga
     1801 cgctggacgc gcaactgggt tccctggggt ggactacagg gtctttgggg ccggatcttc
     1861 gaatggctca gcccgagcga acagaatttg atcccgttgc acgaagcgcg ggtcaacttc
     1921 acgggagggc aatcgatcct cgacttgacg gtctatgaag agagcggcgc taacagccag
     1981 ttccgctttt cactaaccgg caagagtggc aaaaccgaag gcgttttaag caaacttgcg
     2041 cccggccatt tccaagcggt cctgcccgcc ggcgccagcg gcgattaccg catcgacctg
     2101 accgaggagc gaggcggcca gcgcattgcc tacccgccgg tggcctacac gttggcttac
     2161 gatcgcagcg ccgaagtgcc gcgaccggaa ttcaactacc ggttactcgc aagattggcg
     2221 gaggccagcg gcggcgaaat caatccacgc tcactcgacg gcctgcggcg tgaaagtctc
     2281 accaagaact ttcggccggt gcgccatccg ctcttgatca tggctttcgt ccttttgctt
     2341 gtcgaaatcg ccctgcggcg gatcgttttt agggaatcag actaagcttg aattctaagc
     2401 taacaccgcg aattgtccgg ggtattggac ttttgttgac agcgattttg gttgacagac
     2461 cggcctgaac ggattataag ttggaatcct tatttactat ggcttgccaa accacgccat
     2521 ccggccaatt ccttgatgcc cgcctccaaa caccaggaaa atcgattttc ccgagatttt
     2581 tggcgttgta cgtgatcgtt gccgcaatcg cggtggcacc gctcaacggt gaagcgtcca
     2641 agcagctgcc agccgagaaa caggccgtgc gcggcggcaa acaagctccg aagccagcgt
     2701 ccagcacacc ggtaaagcgg ctcaccaagg tccaggctca tcgcgttctg ttgcgccagc
     2761 ctagctcgtc gcccaccaat ctttcggata aagataaata tgaactggtt aattaccggg
     2821 tgcgccccgg cgagactctt tcggacgtgc tgacccgtca caaagtgtcc aaagctgaaa
     2881 ccctgctgtg ggcacgcacc atgacccgaa cggtcggaag tgaaacatta ctatcaggga
     2941 aagagacaaa tcttttcttt ggcaagtcgc tgatgcgcgg tcgcggcccg gtagcccgag
     3001 gcccgctcaa agctgtagag atcgatcaaa acgagtcctt taacctaatc ctggaaaaag
     3061 gcatccgcgg catatatgtc cagagccgtg aaaagcccta cgatgtcgaa gtgaaagcgg
     3121 tcagcgcagc gattgaaaac tcgctcttcg acgatggtcg cagggccggt gtccacccgg
     3181 cgctactgtc ccagcttgcc gatctgttca cctgggatat cgacctggag aaagacattc
     3241 gcaaaggcga tcacttcaag attctctacg agcagcgcag ccgcaagggc caagatacca
     3301 acacagggct acgtattctg gcggctgaac tccatagcgc cggccagaag cacaccgcgg
     3361 tgtattttga gcggcaaaaa ggtgtgggca actattataa ccaagaggga cgcggtctgg
     3421 cacgcgcctt cctacgcttt cccctggaat tcagcgcgat cacgtcgccc ttctccgaat
     3481 cgagatttca tccactcctg cgcacaaacc taccccatac cggagtcgac tttgccgccc
     3541 agcgcggcac cccggttcgg gctgtggggg acggtatcat cgccgaggcg ggttggaacg
     3601 gcggctacgg caaagctata gacatcaaac atgatgcaac ttactccagc cgttacgctc
     3661 atctggatgg atttgccgaa ggtattcaag ccggcgtagc ggtgaccaag ggtcaactaa
     3721 tcggctatgt cggctccact ggcagatcca ccgggccgca cctccatttc gagctctata
     3781 aagatgaaaa atatatcaat cccttaagca tcgactttcc cgcggaagat cacctcgagc
     3841 cggcactgca tcgactcttc gaaaaccaga tgcgtaccta tatggccgag ctcaagtcgt
     3901 ccccccagtc ctagcaaatc cgtccggact ttcgccagca gcccttcccg cctagcgttt
     3961 tcttggttaa ctcaatatta tcgggctcac agtgagcagg ttgcggcata agtttaccct
     4021 ggcgcttgcc accggagctg gggtcggcta ctcgcggttt tttcccggaa cgatggggtc
     4081 cttggtcgcc gtaccaatct cgcttactct caatcgcctg gccgagacca cacccatgtt
     4141 cgcgctaatt gtgttggttg gattgacctt gggggcaatt tggctttcga ccatcgccgc
     4201 ggagatatta caggcgaaag atccgcaaat tatcgtcgcc gacgagatcg tcggctttat
     4261 gattgccaac tttctcgcgc cccggcgctg ggcgccgctg ctgctggtgt tcctgctgtt
     4321 tcgcatcttc gatatcgtca aggtttaccc agcggcacgg ctggaaaaac tccccggcgg
     4381 cctaggcatc gtgcttgacg acgtactggc gggagtctat gcctttgtca tcgtccaaac
     4441 cttactttac tggcggtggt tatgatcgaa aaggcggtta tcttgagtac cggtgatgaa
     4501 ctgaccaccg gcaaagtggt cgataccaat tcagctttca tcgccgatcg cttgttcgca
     4561 ctggggatcc gcgtcgccgc gatactcaag gttggagacg accgcgataa gctgctctgg
     4621 gccttgagtc aagcacagga gctcggcgac atcgtcatcg gcaccggcgg tttggggccg
     4681 acaacggatg atttatccac cgagctgatc gctgattttg ttggcctcaa gactaaactg
     4741 gacgagggcg tcgccgcggc gctcaaagcg cgcttcgcca agcgcggctt gccattcaca
     4801 ccgaacaatc tcaaacaggc gttctttcct gaaggcgcgg caataatccc caatccgaac
     4861 ggtacggcgc ccggcttcca cattgatctt ggtgacggca aaaagctgat ctggctttcc
     4921 ggcgtgcccc acgagatgag tgccatgctg ctcgagtcgg tgctgccatg ggtggtcgag
     4981 cagaccggcg gcggtccgac gacgattcat gaggcgactt tcaaaattca tggcatcagc
     5041 gaaggtaaat tggacgatct ggtaaaaccg ctggatttgg acaacttcgc caaactgtcc
     5101 tttcgcgctc atttccccga tttaactttg cgtctgacga tgagcggcgg tgcggcgcgg
     5161 acggaacatt ttttaaacct gcgcgggcag atcaaaacca tcctc
//
LOCUS       DASQKS010000381         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3143854, whole genome shotgun sequence.
ACCESSION   DASQKS010000381 DASQKS010000000
VERSION     DASQKS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143854"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..797)
                     /locus_tag="VE170_09890"
     CDS             complement(24..797)
                     /locus_tag="VE170_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ15791.1"
                     /translation="MSKRTVQSITLALLIGFAAQISFAAPDEYDDSQSHPLRVLAYAW
                     HPVGWLAEWTIFRPIHYLVSATRPQEQIFGHTPHPPVLAEPQPSYNFGVGKKVPVQTV
                     RVAPPAPAPVPMPEHVKVVQVPVEKTVIQVQEKVVEKIVEVERVVFTPVAFNFDSARL
                     SDLGKGQVFLAAQRLKERGNMTIVIEGHTDNRGDDSYNQKLGEQRAQTVMSELAGFGV
                     DQSRMSTASVGENRPLIGQDTAWARAVNRRVEFQVKSAQ"
     gene            945..1706
                     /locus_tag="VE170_09895"
     CDS             945..1706
                     /locus_tag="VE170_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYJ15792.1"
                     /translation="MSVEVARKDRLLTVTWDRPPLNVLDIVLLRELADTLKRCAADSA
                     VDVVVLQGQGQKAFSAGVDIKDHTKEKVPEMLDVVHGVIRELLRLPQVTIAAVRGACL
                     GGGCEVASVCDFIVASEESSFATPEIFVGCYPPVALARFASLIGYHRAAEMILTGRRF
                     SAREAQAMGFINRVFADNELDNGLKTLVDELLSKSGAVMRLTVKGLRELSMGDFAAAL
                     KRSEQIYCEELLKTADVEEGVQAFLQKRPPQWRHR"
     gene            1766..2536
                     /locus_tag="VE170_09900"
     CDS             1766..2536
                     /locus_tag="VE170_09900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HYJ15793.1"
                     /translation="MADFEIKITTDPRDIIEAQRLRFQVFNLELNRGLQRSYQTGLDV
                     DEFDPFCSHLIVRDNQSNEIVGTYRMMRGAQARQHSGFYSEKEFDLSQIKKLDGELLE
                     LGRSCARKDFRDRALLPLMWEAIAEQVREHQVRYLFGCGSLYTTDLAQVRATFALLKN
                     KYYAPERCRVEPVEGCRFTGLSDVPIGSADEAALFHKLPSLLKGYLRIGALVCGPPAL
                     DAEFGTADFFLLLDFDQLKEGYLKRLGLTDVKSDDAPA"
     gene            2520..3359
                     /locus_tag="VE170_09905"
     CDS             2520..3359
                     /locus_tag="VE170_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ15794.1"
                     /translation="MTRLLKLAAFLSLWAIFFGLVGMVHLWISVLGLPDRWKIISRLN
                     RIYTLLLRAILNIRVTVVGDEGQLERGGYVIIANHVGYIDGIVLGSIFPIVFVSKKEV
                     RSWPIVGQWNILCGTIFIDRQHKGLVGKLVQEISRKLKQEANILLFPEGTSTNGEQIL
                     PFQTVPLAAPLRSRSVIVPTTITYKSIDDAPVSPANRDSLYWYGDMDFMSHFWKLLGL
                     RKVDVLVTIQPKVECFHYRDNSAGRKTLAEDCYNRVLGRLTPGSMDQDEEAEEGGEPL
                     LPA"
BASE COUNT          694 a          903 c          983 g          793 t
ORIGIN      
        1 ttagtgacga tgagtcgatt gtgctactgc gccgatttta cctgaaattc gacgcggcgg
       61 ttgaccgccc gcgcccacgc cgtgtcctgt ccgatcagcg gcctattttc gccaacactg
      121 gccgtcgaca tgcggctctg atcgacaccg aatccagcca gttcactcat gaccgtctga
      181 gcgcgctgct cgccaagttt ctgattgtag ctatcgtcgc cacgattgtc ggtatggcct
      241 tcgatgacaa tagtcatgtt gccgcgctct ttgagccgct gggcggcgag gaaaacttga
      301 cccttaccca agtcggacaa tcgagccgag tcgaaattga aagcgaccgg tgtgaaaacc
      361 acccgctcca cctcaacgat cttctcgacg accttttcct gaacttgaat aacggttttt
      421 tccaccggaa cctggacgac cttaacgtgc tccggcattg gcacgggcgc cggcgccggc
      481 ggggcgacgc gcactgtctg caccgggact tttttgccta cgccgaaatt gtacgacggc
      541 tggggctcag cgagcaccgg cggatgggga gtgtggccga agatttgttc ctgcggtcgg
      601 gtcgcgctga cgagatagtg gatcggtcga aagatggtcc actcggcgag ccagcctacc
      661 ggatgccacg cgtaagcgag tactcttaaa ggatgggatt ggctgtcgtc gtactcatcc
      721 ggcgcggcga agctgatctg cgcggcaaaa ccgatgagta gcgccaaggt gattgactga
      781 actgttcgtt tgctcatttt tcgccctcct cgaggagctg atagaacgtc agtttatgag
      841 ctaggattgg gtttgtcaaa cttttgtttg gcggccgcct tgaagcgcaa ggatcatcgg
      901 tttaatacaa ggccaactag ttggatgacg cctatgaggg aaccatgagt gtagaagtcg
      961 ctcgaaaaga ccgattgctg acggttactt gggacagacc gccgttaaat gtgctcgaca
     1021 tcgtattgtt gcgcgagctt gcggatacgc tcaagcgctg cgcggccgac tctgccgtcg
     1081 acgtcgtggt gttgcaaggc caagggcaga aagccttttc cgccggcgtg gacatcaagg
     1141 atcacactaa agagaaagtt ccggaaatgc tcgacgtggt tcacggcgtg attcgcgagc
     1201 tcttgcgttt gccccaagtt acgattgccg cggtgcgtgg cgcctgtctc ggcggcggct
     1261 gcgaagtcgc cagtgtctgc gattttatcg tcgcttcgga ggaaagttcg ttcgcgactc
     1321 cggaaatttt tgtcggctgc tacccgccgg tggcactggc gcgctttgcg tcgttgatcg
     1381 gctaccaccg ggcggcggaa atgattctca ccgggcggcg ctttagcgcc cgcgaggccc
     1441 aggccatggg atttatcaac cgagtgtttg ccgataacga gttggacaat ggcttgaaaa
     1501 ctctggttga cgagctcctc agtaaaagcg gcgcggttat gcgtctgacc gtcaaaggtt
     1561 tgcgcgagtt gtcaatgggc gattttgccg cagcactcaa acgctccgaa caaatctact
     1621 gcgaggaact gctaaaaacc gccgatgtcg aggaaggcgt gcaggctttt ttgcagaagc
     1681 ggccgccaca atggcgccat cgctaacgcg gcagtgccgc ggattgattt tcaaatggtc
     1741 ggaccatatg ttagttttcc cgcggatggc tgactttgaa attaaaatca ccaccgaccc
     1801 gcgcgatatc atcgaagccc agcgcttgcg ttttcaagtc ttcaatttgg aactcaatag
     1861 ggggctgcag cgctcttatc aaaccggttt ggatgtcgac gaattcgatc ctttctgcag
     1921 ccatttgatc gtccgcgaca accaaagcaa tgaaatcgtc ggcacctatc gcatgatgcg
     1981 gggtgcccag gcgcgccagc attcgggttt ttactcggaa aaagaattcg acttgtcgca
     2041 gatcaaaaaa ttggatggtg aactgcttga attgggccgg tcctgcgcgc gcaaagattt
     2101 tcgcgaccgc gccttgctgc cgctcatgtg ggaagcgatc gccgaacagg tgcgggagca
     2161 ccaagtgcgt tacttattcg gctgcggcag tttatatacg actgacctcg cccaagtcag
     2221 ggcgacgttt gcgctactca aaaataaata ttacgcgccc gaacgttgcc gtgtcgagcc
     2281 cgtggaaggc tgccgcttta ccggtttgtc ggatgtgcca atcggcagcg ccgacgaagc
     2341 ggcactgttc cacaaactgc cgagtttgct caaaggttat ctgcgcatcg gcgcgttggt
     2401 ctgcggtccg ccggcgctgg acgccgagtt tggtacggct gacttcttcc tgcttttaga
     2461 ttttgaccag ttgaaagaag gctatttaaa gcgtttgggc ttgacggatg tgaaaagcga
     2521 tgacgcgcct gcttaaactc gccgcgtttc tttctctctg ggcgatcttc ttcggcctgg
     2581 tcggcatggt tcacttgtgg atctcggtcc tgggcttgcc ggaccgttgg aaaatcatca
     2641 gccgtcttaa tcgcatctat accttgttgt tgcgcgcaat cctgaatatc agggtgaccg
     2701 ttgtcggcga cgagggtcaa ctcgagcgcg gcggctacgt tatcatcgcc aatcacgtcg
     2761 gatatattga cggtatcgtg ctcggcagca tttttcccat cgtctttgtc tccaagaagg
     2821 aagtccgcag ctggcccatc gtcggccagt ggaacattct gtgcgggacg atcttcatcg
     2881 accgccagca caagggacta gtcggcaagc tggtgcagga gatatcgcgc aaattgaagc
     2941 aggaagccaa tatcctgtta tttcccgaag gcacctctac caatggcgaa cagatactgc
     3001 cgtttcagac cgtgccgctg gcggcgccgc tgcgcagccg gtcggtgatc gtgccgacca
     3061 ccatcaccta caaatcgata gatgatgcgc cggttagccc ggccaatcgc gactcgcttt
     3121 attggtatgg cgacatggat ttcatgtcgc acttctggaa gctgttagga ttgcgcaagg
     3181 tcgacgtgtt ggtgacgatc cagcccaagg tcgaatgttt tcactatcgg gataattcgg
     3241 cgggacgcaa aacattggcc gaggactgct acaaccgggt cctcggccgg ttgacgccag
     3301 ggagtatgga tcaggatgaa gaggccgaag agggcggcga gccgttactt cccgcttaac
     3361 tgtttcttga tat
//
LOCUS       DASQKS010000382         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_623369, whole genome shotgun sequence.
ACCESSION   DASQKS010000382 DASQKS010000000
VERSION     DASQKS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623369"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VE170_09910"
     CDS             complement(<1..182)
                     /locus_tag="VE170_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HYJ15795.1"
                     /translation="MKYEPVIGLEVHAQLLTTSKIFCGCSTQFGREPNANTCPVCCGF
                     PGVLPVLNKKVVEFAI"
     gene            complement(284..1741)
                     /gene="gatA"
                     /locus_tag="VE170_09915"
     CDS             complement(284..1741)
                     /gene="gatA"
                     /locus_tag="VE170_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HYJ15796.1"
                     /translation="MNLAALTLHDASEKLRRREISSVELTSAVFQRIDATEEKIHAYL
                     TLARGSAMEQAEQADARLRNDPQVSPLLGIPIAVKDNFLTRELRTTCASKILGDFMPP
                     YDATTVIKLRDAGAVLIGKTNLDEFAMGSSAENSAFFPTHNPWNLKRIPGGSSGGSAA
                     AVAADQCIAALGTDTGGSIRQPAACCGVVGLKPTYGRVSRNGIIAFASSMDQVGPLTK
                     DVRDSALLLQALAGHDPADSTSSNRSVPVYSTGLNGEVKGLRIGISKDYFVPGMDGLV
                     QKAVRDAVLKLENSGAEKVDIGLPSTEYAVAVYYIVATAEASSNLARYDGMRFGHRAQ
                     ARDLTETYMISRAEGFGPEVKRRIMLGTYVLSAGYYDAYYLKAQRVRTLIKRDFEEAF
                     KHCDVIITPTAPTTAFKIGEKIQDPLQMYLSDIYTISANLAGLPAISLPCGFDGDGMP
                     IGLQIIGKPFDEATVLRTAYAYEQATEWRKKKPTL"
     gene            complement(1738..2025)
                     /gene="gatC"
                     /locus_tag="VE170_09920"
     CDS             complement(1738..2025)
                     /gene="gatC"
                     /locus_tag="VE170_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYJ15797.1"
                     /translation="MKLSRESVQRIATLARLRLTADEESEIIGQLDRILSYMDKLNEL
                     DTANIELFYHDIDNLSALREDQLTNQPNTDALLTNAPDRDGNFFKVPKIIE"
     gene            2140..2823
                     /locus_tag="VE170_09925"
     CDS             2140..2823
                     /locus_tag="VE170_09925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYJ15798.1"
                     /translation="MDSGRLESDASKGLVFCPFEADALMQGFHAVAGIDEVGRGPLAG
                     PVVAAAVILPCGFTHPEIKDSKMLSAKQREKLALVIQQVAQSWALGVIDVDGIDRLNI
                     LRASLTAMALALQCLTPKPDYVLIDGNQKIPLEFFAEFGSNSAQLAQQTIVKGDQLCL
                     SIAAASIVAKVARDQMMFNFDKQYPEYGFAGHKGYASAAHLAALQRYGPTPIHRRSFK
                     PVRDLLIIA"
     gene            2862..3227
                     /locus_tag="VE170_09930"
     CDS             2862..3227
                     /locus_tag="VE170_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYJ15799.1"
                     /translation="MSFFRKRLGQEGEDLAAGFLRKKGYKLVERNYRCKTGEVDLIVL
                     DRKVVVFVEVKTRSDHSFGSPFEAVEARKQRKMIQAAQYFLHERKLHQRDARFDVVGI
                     SWQGDRPVLEHIENAFEVK"
     gene            3240..>3588
                     /locus_tag="VE170_09935"
     CDS             3240..>3588
                     /locus_tag="VE170_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYJ15800.1"
                     /translation="MLDVKLLRDDLAQVKERMATRGGAVDWDAFVAIDRERRDALATI
                     ERLKEKKNRLSGEIGKIKKAGGDAGALMRETEAVSEAIHVSEAPLADIEARFESFMLS
                     VPNLPSPTVKIGKS"
BASE COUNT          767 a          915 c         1091 g          815 t
ORIGIN      
        1 ttgatggcga actcgacgac tttcttattg agcactggca atacgccagg aaagccgcag
       61 cagactgggc aggtgttggc gttgggttcg cggccgaact gtgtcgaaca accgcaaaag
      121 atttttgatg tggtgagcaa ctgggcgtgg acttccaggc cgatgacggg ctcgtatttc
      181 attggcgaaa gttccgaaat cgggaattga gaattgagga cgcgagcttt cggctcgcca
      241 gtgcgagtct ctcatgtttc ataatcaatt ccccattgtc cgtctaaagc gttggttttt
      301 tcttgcgcca ctcagtcgcc tgttcgtagg cgtacgccgt tcgcaatacg gtggcttcgt
      361 cgaaaggctt gccgatgatt tgcaatccaa tcggcatgcc gtcgccgtca aaaccgcaag
      421 gcaatgagat ggctggtaag ccggccagat tggctgagat cgtgtagatg tcggaaaggt
      481 acatctgcag cggatcttgg atcttttcgc cgattttgaa cgccgtcgtc ggcgctgtcg
      541 gcgtgatgat cacatcgcaa tgtttaaagg cttcttcgaa gtcacgcttg atgagagtgc
      601 gcacgcgctg ggccttgaga taataagcgt cgtaataccc cgccgacaac acgtaagtgc
      661 caagcatgat acgccgtttc acttcggggc cgaaaccctc agcgcggctg atcatgtacg
      721 tttccgtgag gtctcttgcc tgagctcgat gaccgaaacg catgccgtcg taacgggcta
      781 gattcgaact cgcttcggcg gtggcgacaa tatagtaaac ggcgacggcg tattccgtag
      841 atggcagacc aatatcaacc ttctcggcgc cgctgttttc cagtttgagg acggcatctc
      901 gcaccgcttt ttgcactaac ccgtccatgc ctgggacgaa gtagtcttta gaaattccga
      961 tacgcaggcc tttaacctca ccattcaacc cagtgctata aacgggaact gagcggttcg
     1021 atgatgtgga atcggcggga tcgtggccgg cgagcgcttg tagcagtagt gccgagtcgc
     1081 gcacatcttt ggtcagtggt ccgacttggt ccatggacga ggcgaaggcg atgatgccat
     1141 tgcggctgac ccggccgtag gtaggtttca agccgaccac gccgcagcaa gcggcaggtt
     1201 ggcggatcga gccgccggtg tcggtgccga gagcggcgat gcattggtcg gcggcaacag
     1261 cggcggccga gccgccggag gaaccgccgg ggatgcgttt aagattccac ggattgtgcg
     1321 tgggaaaaaa cgcggagttc tctgccgagg atcccatggc aaactcgtca agattggtct
     1381 tgccgatgag gacagcgccg gcgtcgcgta gtttgatgac ggtcgttgcg tcataaggcg
     1441 gcataaagtc gcccaaaatt tttgacgcgc atgtggtgcg caattcgcga gtgagaaagt
     1501 tatctttgac agcgatcggt atgcccaata gcggcgagac ttgcggatcg ttgcgcagac
     1561 gcgcatcagc ttgctccgcc tgctccatag cgcttccacg ggccaatgtg agataggcgt
     1621 gaattttctc ctcggtggcg tcaatccgtt gaaacaccgc gctggtgagc tcgacggacg
     1681 agatctcgcg acggcgaagc ttctcgctgg cgtcgtgaag tgtcagcgcg gcaagattca
     1741 ttcgatgatc ttcggaacct tgaagaagtt tccatcccga tccggcgcgt tggtgagcag
     1801 cgcgtcggtg ttgggctggt tcgttagctg gtcctcgcgc agggccgata aattgtcgat
     1861 atcatgataa aatagctcga tgtttgccgt gtcgagctcg ttgagcttgt ccatatagct
     1921 tagtatccga tcaagctgcc cgatgatctc gctctcctca tccgcagtca ggcggaggcg
     1981 cgccagggtt gctatccgtt gcaccgattc acgggacaat ttcatggaaa ttttttaaca
     2041 tacggctgtc gccaatgcca ccggctgggc ccgggtgttg ctggtgtcgc ggaagaaaat
     2101 atttgcagtt actgggggtt cgggctaaaa gtgaaaccta tggacagtgg tcgattagaa
     2161 tcggacgcga gcaaaggatt ggtattttgc ccattcgagg ccgacgccct gatgcagggc
     2221 ttccatgcgg tggctggaat tgatgaagtc gggcgtggac cgctggcagg gcccgtggtc
     2281 gccgcggcag tgattttgcc gtgcggtttt acccatccgg agatcaagga ttcgaagatg
     2341 ctcagtgcaa aacagcgcga aaaattggcg ctggtcattc aacaggtggc gcaaagctgg
     2401 gcattggggg ttatcgacgt cgatggcatc gaccgtttaa atattctacg cgccagtctg
     2461 accgccatgg cgttagcgct tcaatgccta acgcctaagc cggattatgt gctcattgac
     2521 ggcaatcaga agatcccgct ggaattcttc gccgagttcg gttccaactc ggctcagcta
     2581 gcccagcaaa ctatagtcaa gggcgatcag ctctgtctgt ctatcgctgc cgcgtcgatt
     2641 gtcgccaaag tggcgcgcga ccagatgatg tttaatttcg acaagcagta tcccgagtat
     2701 ggtttcgccg gtcacaaagg ttatgcgagt gcggcccatt tggcagcgct gcaacgctac
     2761 ggtccgacgc cgatccaccg gcgcagcttc aagccagtac gtgatttatt aatcatcgct
     2821 taggaggagt agccggagcg ggccgctctg gtgcagcgtc ggtgagtttt tttcgaaaac
     2881 gtctcggtca agagggcgaa gatctcgccg ctggcttctt gcgcaaaaaa ggctacaaac
     2941 tggtcgagcg caactaccgc tgtaagaccg gcgaagtgga tttgattgtc ctcgaccgca
     3001 aagtcgtggt cttcgtcgag gtgaaaactc gctcggatca cagctttggc tcaccgttcg
     3061 aagcggtgga agcgcgcaag cagcgcaaga tgattcaggc ggcgcagtat ttcctgcatg
     3121 agcgcaaact acaccagcgc gacgcaaggt tcgatgtcgt cggcatttct tggcagggcg
     3181 accgtccggt gctggagcat atcgaaaacg cttttgaagt gaagtaggtg ggtcgattca
     3241 tgttggatgt aaaattattg cgcgatgatc tcgctcaagt aaaagagcgc atggcgaccc
     3301 gcggcggcgc ggtcgattgg gatgcattcg tcgccatcga ccgcgaacgg cgtgacgcct
     3361 tggcgactat cgaacgcttg aaagaaaaga aaaaccgcct gtccggcgag atcggcaaga
     3421 ttaagaaggc cggcggcgat gccggagcgt tgatgcgcga gaccgaagcg gtcagtgaag
     3481 cgattcacgt cagtgaggcc cccctggctg atattgaagc gcgctttgaa tcgttcatgt
     3541 tgagcgtgcc caatcttccg agtccgacgg tcaaaatcgg caagagcg
//
LOCUS       DASQKS010000383         7242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1466014, whole genome shotgun sequence.
ACCESSION   DASQKS010000383 DASQKS010000000
VERSION     DASQKS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466014"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VE170_09940"
     CDS             <1..120
                     /locus_tag="VE170_09940"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ15801.1"
                     /translation="KYSTEPKIGGLIASVGQRMLNGVAKMMADQFFKKVESFI"
     gene            171..1076
                     /locus_tag="VE170_09945"
     CDS             171..1076
                     /locus_tag="VE170_09945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ15802.1"
                     /translation="MIPTVSVIIPTYNRWATVGQAVASVLEQRYRDFELIVVDDGSSD
                     RTAAELARFGGQLHYVFQARSGVSAARNLGVRRSRGRLIAFLDSDDLWRPDKLLVQVA
                     FMEEYPEVQICQTEEIWIRHGIRINPKLKHRKPSGDIFEPSLELCLISPSAVMTTREL
                     FDRSGGFDESLPVCEDYDLWLRIARDYPVPLIDQALVVKHGGHADQLSHSRWGLDRYR
                     VVALQKLLRAGIAGPQRRAVLGVWQKKIFVLANGARKRGKVADANGYLAMLSEFAEEM
                     SDVGEWVSKFRGGEGLPSADTGALA"
     gene            1003..1554
                     /locus_tag="VE170_09950"
     CDS             1003..1554
                     /locus_tag="VE170_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15803.1"
                     /translation="MSESGFQSFAAAKGFHPQTLARWLNWPAQARGVLAQLALDLKIG
                     ENQLRDLMDWGEEIALRDGIAIEAIFAGKAITTIATDPRLGRADKVKRIKESLRRLRF
                     PRLAQAEDEIHGKIRALKLQPEIRMTVAPGLEGGRLQIEFTVTNQQDFKRLVEKLAVA
                     AETISTKEIFSLLGGSATSAEPA"
     gene            1575..2669
                     /locus_tag="VE170_09955"
     CDS             1575..2669
                     /locus_tag="VE170_09955"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ15804.1"
                     /translation="MKDLQPEEVIVERGSEGSPILQNLRRTLPQIPFRFVDDIAASNG
                     SAGVVGDSFGAAKKKLYLAKHKGEFLKKCPGSDGQVCCNYFVINFASNCPMDCSYCYL
                     QEYLADNSALKVFSNVGDLIDEVEATLKKHRGVFFRIGTGEITDSLALEPYTGMVRQI
                     LPFFAEQPNVLLELKTKSDCIDSLLDLDPKGRVVVGWSMNPQRVIDADEHATASLTER
                     LKAARRVQAAGYRLGFHFDPMIEYSGWEADYQAMLEETFATIDWRGLSWVSLGVLRDT
                     PGLKRTMRERFPGSRLLTGEQVLCPDGKLRYYQPLRVQMYRQMVSWIRHAAPTVKVYL
                     CMESREVWEQVFGFAPSCEKELGNQMAPSS"
     gene            2707..3480
                     /gene="murI"
                     /locus_tag="VE170_09960"
     CDS             2707..3480
                     /gene="murI"
                     /locus_tag="VE170_09960"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYJ15805.1"
                     /translation="MFDSGIGGLTVLHQIIEALPRENTVYLGDTARSPYGTKSVETVM
                     RYSCENSEFLVEKGVKLVVVACNTSTAIALSHLQESLSVPVIGVIEPGVRRAIKSTKN
                     RKVGVIGTEATIQSGAYTRALKAADPKIEVYSRACPLFVPLVEEGWTENAVVEMAIKA
                     YLSSLKQSGIDTLVLGCTHYPLLKKAIRKFMGPGVRLVDSAEETASEVAAALKKGASG
                     RKAGKGIHSFFVTDAPERFVKVGRRFLGENVESAVRIER"
     gene            3598..7197
                     /gene="secA"
                     /locus_tag="VE170_09965"
     CDS             3598..7197
                     /gene="secA"
                     /locus_tag="VE170_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HYJ15806.1"
                     /translation="MWDFIKKIVGTKNDREIKRIRPYVDEINKLEPEYQGLNDDDLKA
                     KTDQFRRRLDEATATLKSELTEAQNRASVAEPDEREELKTQAEELDKELRQKEAEILD
                     QLLPEAFAAVREASRRTIGLRHYDVQLIGGAVLHSGKIAEMKTGEGKTLVATLALYLN
                     ALTGRGAHLITVNDYLARRDVQWMGPIYHKLGLTAASIVHEVSYLFDPTYLTKDYRFL
                     NLRPIQRQEAYHADITYGTNNEFGFDYLRDNMKFSLEEYVQRELNFVVVDEVDNILID
                     EARTPLIISGPAEESTDKYYLIDRVIPKLQRGAVIQGDPSQEDRAAVEAKGDYTVDEK
                     SRSVTLTETGVAKVERLLGVHNLYDPRQIDTLHHVQQALKAHAIFKRDVDYVIKEGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKERVHIREENQTLATITIQNYFRMYKKLAGMT
                     GTADTEAPEFQKIYKLDVTVVPTHRNMVRQDNADVVYRTDNEKYDAVVLEIEERHKHG
                     QPVLVGTTSVAKSEKLSRLLKKKNIKHNVLNAVNHEAEASIIAQAGRYGALTIATNMA
                     GRGTDILLGGNPEFLARSDMENEWINRASKLPLQGAARYEDSLRELREKYEEQVQGAE
                     KKFLKDSELFDQQRSAALKRTTELQREIRIASPFGEAREKYEDVSATQLIEALHGARP
                     IPEGYLKAKESFEGAVVAANNGQAASLPDEFVEARDEFQATLDRWQQRADERNALTEF
                     LDEKRRVYEQRLIDLEFDRAKAALAQGDLAGAVDEYEQSHRSFEEAEARCEELRLPYE
                     KAIQDAQEDYETKRQEYLRMVEAIRDQLEKAPDEYRLRFDEMLAQYNKSCAEEKDKVI
                     AAGGLHILGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFGADRIQGIM
                     SRLGMEEGEPIEHGLVTRAIENAQKKVEAHNFDIRKHLLEYDDVLNKQREVIYGHRRE
                     VLKGETLKEQVTEWADELAEEIIARCAEKDRHPSDWDWNALREGMNHQFNIRPEFASD
                     EIEQVTEGQLADTVHQKILEKYEDKEKRFGEPMLRQLEKIIMLQTIDTLWKDHLLSMD
                     HLKEGIGLRGYGQKNPLQEYQKEGYDMFEDMNHRVQEDVVQKLFTVELARENAQQEVA
                     VEQPRQQRMVLSHGQDPIAAQPNPAPRRETAKVGRNDVCPCGSGKKYKRCHGK"
BASE COUNT         1715 a         1905 c         2168 g         1454 t
ORIGIN      
        1 aagtacagca ccgagccgaa gatcggcggg cttatcgcta gtgtcggcca acgcatgctc
       61 aacggcgtcg ccaagatgat ggccgaccaa ttttttaaaa aggtcgagag cttcatctaa
      121 agttttttgg cttcgtccta cgcgcctgac ttttcggtga gcttacgtcc atgataccca
      181 ccgtcagtgt catcattccg acctataacc gctgggccac ggtggggcag gcggttgcgt
      241 cggtgttgga gcaacgctat cgcgacttcg agctgatcgt ggtcgatgat ggctccagcg
      301 atcgaactgc cgcggagctg gccaggtttg gtgggcaatt gcactacgtc ttccaggctc
      361 gaagcggggt gtcggcggcg cgaaacctgg gcgtccgccg gagccgcggc cggttgatcg
      421 cgtttctcga ttcggacgat ctctggcgcc cggacaagct cctcgtgcaa gtggctttta
      481 tggaggaata tccagaggtg cagatttgcc aaaccgagga gatctggatt cgccacggta
      541 tccgcatcaa tccaaagctc aagcatcgca agccgtcggg agatattttc gagcctagtc
      601 tcgaactctg tttgataagt ccgtccgcgg tgatgacgac gcgggaattg ttcgaccgca
      661 gcggcggctt cgacgagtcg ttgccggttt gcgaagacta cgacctctgg ctgcgtatcg
      721 cccgggatta tccggtgccg ctgatcgatc aggctttggt cgtcaaacat ggcggccatg
      781 ccgaccaatt gtctcattcg cggtggggct tggaccgtta tcgggtggtt gcgctgcaaa
      841 agcttttacg cgccggcatc gccggcccac agcgccgcgc ggtattgggg gtttggcaaa
      901 aaaagatttt cgtgctcgcc aacggggcgc gcaagcgcgg caaagtggcg gacgctaatg
      961 gatatctagc gatgctcagc gagtttgccg aggagatgag cgatgtcgga gagtgggttt
     1021 caaagtttcg cggcggcgaa gggcttccat ccgcagacac tggcgcgctg gcttaactgg
     1081 ccggcgcagg ctcgcggcgt gctggcacag ctcgctttgg acctcaagat cggcgaaaat
     1141 cagctgcgcg acttgatgga ttggggagaa gaaatcgctt tgcgcgacgg catcgccatc
     1201 gaggcgatat ttgccggcaa agccatcacc actattgcca ccgatccgcg cctgggccgt
     1261 gcggacaagg tgaaaagaat taaggaaagt ctcagaagat tgcgctttcc gcgcttggcc
     1321 caggccgaag acgaaatcca cggtaaaatt cgcgcgctca agctgcaacc ggagattcgc
     1381 atgacggtgg cgcctggatt ggagggcggc aggttgcaga tcgagtttac cgtgaccaac
     1441 cagcaggatt ttaaacgact ggttgaaaag ttggccgtcg ccgccgagac gatttctact
     1501 aaagagattt tttcgctctt aggcggtagc gcgacgagtg ccgaacccgc ttagtgtagt
     1561 tgacggcagg cccaatgaaa gatttacaac ccgaagaagt gatcgtcgag cgcggctccg
     1621 aaggcagccc catcctgcaa aatttgcgtc gcactttacc gcagattccg tttcgctttg
     1681 tcgatgacat tgctgcatcg aatggcagcg ccggcgtggt cggcgactcc tttggcgcgg
     1741 ccaagaaaaa gctttatctg gcaaagcata agggcgaatt cttgaaaaaa tgtccgggca
     1801 gcgatggaca ggtgtgctgc aactatttcg tcatcaattt cgccagcaac tgtccgatgg
     1861 attgcagtta ttgttatttg caagagtact tggccgataa ctctgcgctc aaggtcttca
     1921 gcaacgtcgg cgacttgatc gacgaggtcg aagcgacctt gaaaaagcat cgcggggtat
     1981 tttttcgcat cggcaccggc gagatcaccg atagtttggc gttggagccg tataccggca
     2041 tggtgcgaca gattttgccg ttctttgccg agcagcctaa cgtgctcttg gagctcaaga
     2101 ccaagagcga ctgcatcgat agtttgctcg atctcgatcc caaggggcgg gtggtcgtgg
     2161 gctggtcgat gaatccgcag cgggtgatcg acgccgatga gcatgctacc gcttccttga
     2221 cagagcgctt gaaggcggca cggcgcgtcc aagctgccgg ctaccggctg ggctttcatt
     2281 tcgatccgat gatcgaatat tccggctggg aagccgatta tcaggccatg ctggaagaga
     2341 ccttcgcgac cattgactgg cgtggacttt cctgggtcag tctcggcgtc ctgcgcgaca
     2401 cgccgggttt gaaacggacc atgcgcgagc gcttcccggg cagccggttg ctcaccggcg
     2461 aacaggtgct ctgtcccgac ggcaagctgc gatactatca gccgctccgg gttcaaatgt
     2521 accgccagat ggttagctgg attcgccatg ctgcgccaac ggtgaaggtc tatctatgca
     2581 tggagtcccg cgaagtgtgg gagcaggtct ttggttttgc gccgtcctgc gaaaaagagc
     2641 tgggcaatca gatggcgccg tcgagctaaa ttgcgatcga taataaacaa agatagtgca
     2701 atcggggtgt tcgactcggg cattggtggg cttaccgtac tgcatcaaat catcgaagca
     2761 ttgccgcgcg agaacacggt ttatttggga gacacggcgc gctcccccta tggcaccaag
     2821 tcggtggaaa ccgtcatgcg ctactcttgc gagaacagcg aatttttggt ggaaaagggc
     2881 gtcaagctgg tggtcgtcgc ctgcaatacc tctacggcca tcgcgctgag ccatttgcaa
     2941 gaaagccttt cggtgccggt cattggggtg atcgaacccg gggtgcgtcg ggcgatcaag
     3001 agcaccaaga atagaaaagt cggcgtcatc ggcaccgaag cgacgattca aagcggcgcc
     3061 tacacccggg ccttgaaagc cgcggacccc aagatcgaag tttacagccg ggcttgtccg
     3121 ctgttcgtgc cgctagtcga agaaggttgg accgagaacg cggtggtcga gatggctatc
     3181 aaagcgtatc tcagcagtct caaacagagc ggcatcgaca cgttggtatt aggttgtact
     3241 cactatccct tgctcaaaaa ggcgatccgc aagttcatgg gcccgggggt gcgtctggtc
     3301 gactccgccg aagagaccgc cagcgaagta gctgcggcac tcaaaaaagg tgcgagtggg
     3361 cgcaaggcgg gtaaaggaat tcacagtttt tttgtaaccg atgcgccgga gcgcttcgtc
     3421 aaagtcggtc ggcggtttct tggggagaac gtcgaatcgg cggtacggat cgaacgctaa
     3481 gctcccttgc gctcctagac tagctttaag tcgctggtca attcgtgtat tttaataagg
     3541 tctttagcag cccaatggct cctccatttc gagtgaattc gggagtaaat ttttaacatg
     3601 tgggatttca tcaaaaaaat tgtcggcacc aaaaacgacc gcgagatcaa gcgcatccgt
     3661 ccctatgtcg atgaaatcaa caaactcgag ccggaatacc aggggctcaa cgatgacgac
     3721 ctcaaagcga agaccgatca gtttaggcgc cgactcgatg aagccacggc gactttgaaa
     3781 agcgagttga cggaggccca aaaccgcgcc agcgttgccg agccggacga gcgcgaagaa
     3841 ctcaagaccc aggccgaaga gcttgacaag gagctgcgcc aaaaagaggc cgagatacta
     3901 gaccaactgt taccggaggc gttcgcggcg gtgcgcgaag cgtcacggcg caccattggc
     3961 ttgcgccatt acgatgttca gttgatcggc ggcgcggtgc ttcactccgg caaaattgcc
     4021 gaaatgaaaa ccggcgaggg taagacgttg gttgcgacct tggcgctgta tttgaatgcg
     4081 ctcactggcc ggggcgcgca cttaatcacc gtcaacgact acttggcgcg gcgcgatgtc
     4141 cagtggatgg ggccgattta ccataagttg ggactcaccg cggcctccat cgtgcacgag
     4201 gtcagttatc tattcgatcc cacttatttg accaaggatt atcgtttttt gaatctgcgg
     4261 ccgattcaac gccaagaggc ttaccacgcc gatatcactt acggcaccaa taacgagttc
     4321 ggcttcgact atctgcgtga caacatgaag ttctccctgg aagagtatgt ccagcgcgag
     4381 ctcaacttcg tcgtcgttga cgaggtagat aatatattga tcgacgaagc gcgcacgccg
     4441 ttgattattt ccggaccggc cgaggagtcg acggataagt actatctcat cgaccgggtg
     4501 atccccaagt tgcagcgggg cgcggtgatt caaggtgatc catcgcagga agaccgcgcc
     4561 gccgtcgaag ccaagggcga ttacaccgtc gacgaaaaaa gccgctcggt gaccttgacc
     4621 gaaaccggcg ttgccaaagt cgaacgtctg ttaggcgtgc ataaccttta cgacccgcgt
     4681 caaatcgata ccttgcacca tgttcagcaa gcgctcaaag cccacgcgat tttcaaacga
     4741 gatgtcgact atgtcatcaa agaaggcgaa gtaatcatag tcgacgagtt caccggccgt
     4801 ttgatgccgg ggcggcgttg gtccgacgga ctgcaccagg cggtggaagc taaggagcgt
     4861 gttcatattc gcgaagaaaa ccagaccctg gcgaccatca cgattcaaaa ttacttccgc
     4921 atgtacaaaa agctcgcggg catgaccggt accgccgata ccgaggcgcc cgagtttcaa
     4981 aaaatttaca agcttgatgt caccgtagtg ccgacccacc gcaacatggt gcgtcaggac
     5041 aatgccgacg tggtttatcg tacggacaac gaaaaatatg atgccgtggt gttggagatc
     5101 gaagagcggc acaagcacgg gcagccggtg ctggtcggca ctacctcggt ggccaagtcg
     5161 gaaaagctat cgcgcctgct caagaagaaa aacataaaac acaatgttct gaacgcggtc
     5221 aatcatgaag cggaagcgag cattatcgcc caggccggtc gctatggggc gctcaccatc
     5281 gcgacgaata tggccggccg cggtacggat atattgctag gcggcaatcc ggaatttctc
     5341 gcgcgctcgg atatggaaaa cgaatggatc aaccgggctt cgaaattgcc gctgcagggc
     5401 gcagcacgct acgaggactc gctacgggaa ttgcgcgaaa agtacgaaga gcaggtccag
     5461 ggcgccgaga agaaattcct caaggacagc gaactgttcg atcagcagcg cagcgcagcc
     5521 ttgaaacgca ccaccgaatt gcaacgcgag atccgtatag cgtcgccctt cggcgaggcc
     5581 agagagaagt acgaagatgt ctcggccacc caattgatcg aagctttgca cggcgcgcgg
     5641 ccgatcccgg aagggtactt gaaggccaaa gagagctttg aaggcgcggt ggtggcggcg
     5701 aacaacggac aggcggctag cttgccggac gaatttgtcg aggctcggga cgagtttcag
     5761 gccactctgg accgctggca gcaaagggcc gacgagcgca acgcgttgac cgagtttctc
     5821 gatgaaaaac ggcgtgtcta tgaacaacgg ctgatcgatc tggaatttga ccgtgccaaa
     5881 gcggcactcg ctcaaggcga tttggccgga gcggtcgatg aatatgaaca gtcccatcgg
     5941 tcgtttgagg aggcggaggc acgttgcgag gaactccggt tgccctacga aaaggcaatt
     6001 caggacgctc aagaagacta tgagaccaag cgccaggaat atttgcgcat ggtcgaggct
     6061 attcgcgacc aactggaaaa ggcgccggat gagtaccgtc tccgttttga cgagatgttg
     6121 gcgcagtaca ataaaagttg cgccgaggaa aaggacaaag tgatcgccgc cggcgggttg
     6181 catatcctgg gtaccgagcg ccatgaaagc cggcgcatcg ataatcagct acgcggccgg
     6241 gctggacgcc aaggcgatcc cggctcgtcg cgattctacc tttccctcga ggacgatctc
     6301 atgcgcatct tcggcgcgga ccggattcaa ggcatcatga gccgattggg tatggaagaa
     6361 ggcgagccga tcgagcatgg gctagtcacc cgcgccatcg agaatgccca gaagaaggtc
     6421 gaggcgcaca acttcgatat ccgcaaacat ctactcgagt atgacgacgt gctcaacaag
     6481 cagcgcgaag tgatttatgg ccatcgacgc gaggtgctca aaggcgaaac tttgaaagag
     6541 caagtgaccg agtgggccga tgaactcgcc gaagagatca ttgcccgttg cgccgaaaag
     6601 gaccgccacc cttcggattg ggactggaac gcactgcgcg aagggatgaa ccatcaattc
     6661 aacatccgcc ccgagtttgc cagcgatgaa atcgaacaag tgactgaggg gcagcttgcg
     6721 gacacggttc accagaaaat tctggaaaag tacgaagata aggaaaagcg gtttggcgag
     6781 ccgatgttac gccagctgga aaagatcatc atgttgcaga ccatcgatac tctttggaaa
     6841 gaccatttgc tcagcatgga ccacctcaag gaaggcatcg ggttgcgcgg ctatggccag
     6901 aagaatccgt tgcaagagta tcaaaaagag ggctacgaca tgttcgagga catgaatcac
     6961 cgtgtccaag aagacgtcgt gcaaaaactt tttacggtgg aactagctcg ggaaaatgcc
     7021 cagcaagagg tcgccgttga acagccgcgc caacaacgca tggttctcag tcatggccaa
     7081 gatccgatcg cggcccagcc caacccagcg cctcgccgtg agaccgccaa agttgggcgc
     7141 aacgatgtat gtccctgcgg cagcggtaag aaatataagc gttgccacgg caaatagtct
     7201 gacatttcag cggcgctggc agggcttgac gtcacgtgtg gc
//
LOCUS       DASQKS010000384         4993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1874784, whole genome shotgun sequence.
ACCESSION   DASQKS010000384 DASQKS010000000
VERSION     DASQKS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874784"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="secD"
                     /locus_tag="VE170_09970"
     CDS             <1..879
                     /gene="secD"
                     /locus_tag="VE170_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYJ15807.1"
                     /translation="SSPAKVPYVLESRTLMTGEYIKDARVRPGEQLQGPYVELILNDQ
                     GGRLFEQITAANVKRRMAIVLDNNVYSAPVIQERIGGGRASITGSFDIKEARDLSIVL
                     RAGALPAPVEIVEERTVGPSLGRDSITQGLTSFVVGGALVILFMVAYFKGAGLVADVA
                     LVFNVLFMAAILAGFQAVLTLPGIAGIVLTLGMAVDANVLINERIREELRAGRAVRSA
                     IEAGYQNALPAILDSNITTFLSGVILFQFGTGPIKGFAVTLCIGILTTVITAVYMTRI
                     YYDYRIGARRLQSISI"
     gene            887..1810
                     /gene="secF"
                     /locus_tag="VE170_09975"
     CDS             887..1810
                     /gene="secF"
                     /locus_tag="VE170_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYJ15808.1"
                     /translation="MDIIKPGSKIPFTRYRKIALIASMAVNLAVLAWLFAKGPNLGVD
                     FAGGTMVQLKFQQRTTIPDIRQALQTIDLGGSTIQEFGQEGAFEFLVRVEKVGVEIAA
                     LGDQMKKALTAKFGADKFEIRRVEFVGPKIGEDLRWRGTLSVIAAMVMMGVYIWVRFA
                     SAFGAKFGLHFGLGAVIALMHDVLVTVGALMLANYEFDLTVVAALLTIVGFSVNDTVV
                     ICDRIRENLRKIRRESLESIINTSLNETLSRTILTTGTALLVLLALYLLGGAVIRPFA
                     FALLVGFVSGVYSTIFIASPVILLWEKSFRR"
     gene            1960..3723
                     /gene="recJ"
                     /locus_tag="VE170_09980"
     CDS             1960..3723
                     /gene="recJ"
                     /locus_tag="VE170_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HYJ15809.1"
                     /translation="MPHDLAKRWRLKPSDEATVMRFAAELKLSPLIARILTLRGFIDN
                     DATRCYLSSSLRSDLPSPFEMADMDAAVERIAAAVTSNEQIAIWGDYDVDGTTGASVL
                     VSFLREVGAKPFYHVPHRIEEGYGLNLEGLKALKERGAGLVITVDCGISNAKEVAAAN
                     GFGLDLVVVDHHQPPEVLPPAVAVVNPHRKDCRFPDKGMCAAGLAFYLVIGLRAKLRE
                     AGWFKHGAAPDIRHFLDIVTLGTIADMVPLRGVNRTLIKRGLTELGISTRPGVVALKK
                     VAGIPDGDVTAGQVGFRLGPRINAAGRVDYGIKVVELLTTESSEIAQRIAQELEEHNL
                     ERRAVEAEVQQRALAQAEAAVAAGSCYSLVLAGAGWHPGVLGIVASRVVERFYRPTVV
                     IGFDNGVGKGSARSIRGFNIVEAFHVCAAHLEKFGGHEFAGGLSIRPECLEPFAAAFE
                     KAARQTLSNEQLSPLLQIDAQLDFSQLTLPLMRELEVMQPFGVGNPEPMFTTVNAEIC
                     ERKVFSAGVRFRLRQAGKVVSGVIFGVGEEYPGQPGESVDVAYRLSENQWNGNTTVEL
                     KIIDMRMAKPGFAVIVRAAGD"
     gene            3761..4762
                     /locus_tag="VE170_09985"
     CDS             3761..4762
                     /locus_tag="VE170_09985"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15810.1"
                     /translation="MFKALLFSTFLMHASVQVGLAAEPGGKPEKPDVIVTYAQPSGAF
                     TPIWVAYEAGLFKKYGLNATLQLLNPQVSAQAVIAEEADFYTNGPDLINARLHGGQVK
                     YFGGTIQQLVFQIWGAKEIKTIQDLKGKTVAASTPRAALDTATREALKKNGLVPDKDV
                     QILYVQSVPAILSSILGGKTAAGTLSAPNTVKAKESGLNLLVDIGKLNIPAFQVTYGA
                     TEKYLKNNPNTVYAFLKAIAEGVVLSRNDPAVAKKAIGKYVKIDDAPTIDGTYDAFAP
                     YWAMSLAVRPEAIRGQFEYIDEKEFPKVKNADPKEFYDNSFVEALTKSGFLKSLGMK"
     gene            4794..>4993
                     /locus_tag="VE170_09990"
     CDS             4794..>4993
                     /locus_tag="VE170_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HYJ15811.1"
                     /translation="MGIQSRRDIVYLALAGFFVTNAILGELTGGKLFTLGPFTLSIGV
                     IPWPIVFIATDLINEYFGREGV"
BASE COUNT         1027 a         1368 c         1503 g         1095 t
ORIGIN      
        1 tcaagcccgg caaaagttcc ctatgtcctg gaatcgcgca cgctaatgac tggagaatat
       61 atcaaagacg cgcgggtgcg tcccggcgag cagctgcaag gcccctatgt cgaactcatt
      121 ctcaacgatc aaggcgggcg gcttttcgaa cagatcaccg cggctaacgt gaagcggcgc
      181 atggccatcg tgctcgacaa caatgtctac tcggcgccgg tgatccagga gcgcatcggc
      241 ggcggccggg cctcgatcac cggcagcttc gacatcaaag aagcgcgcga tctatcgatc
      301 gttctgcgcg ccggcgcgct gccggcgccg gtggagatcg tcgaagaacg caccgtcggg
      361 ccatcgttgg ggcgcgactc gatcactcag ggcctgactt cgtttgtcgt cggcggcgcg
      421 ttggtgatcc tgttcatggt cgcatacttc aagggcgccg gcttggtcgc tgacgttgcg
      481 ctggtcttca acgttttgtt tatggctgcc atcctagccg gtttccaagc tgtattgacg
      541 ctcccgggca tcgctggaat tgtcttgacc ctcggcatgg cggtggatgc caatgtgctc
      601 atcaacgaac gcatacgcga ggaactccgc gcgggacggg cagtccgttc tgctatcgaa
      661 gccgggtatc aaaatgccct gccagcgatc ttggactcca acattacgac gtttctgtcg
      721 ggtgtgattc tctttcagtt cggcaccggc ccgattaaag gtttcgccgt gacgctgtgc
      781 atcggcatcc tgacgaccgt aatcacggcg gtctatatga cccgtatcta ttacgactat
      841 cgcatcggcg cgcgccgact gcagagcatc agcatctagg gaagcaatgg atatcatcaa
      901 gcctggcagt aagatcccgt ttactcgtta tcgaaaaatc gctctgatcg cttcgatggc
      961 cgtgaacctg gcggtgctcg cctggctctt cgctaagggg ccaaatctgg gcgtcgactt
     1021 cgccggcggc acgatggtgc aattgaagtt tcaacagcgg acgacgatcc cggatatccg
     1081 gcaagctttg cagacgatcg atctcggtgg cagcacaatt caagaatttg gccaggaagg
     1141 cgcttttgaa tttctcgtcc gcgtggaaaa ggtcggggtc gagatcgctg cgctgggcga
     1201 tcagatgaaa aaggcgctaa cggcaaagtt cggcgccgat aaattcgaga tccgccgcgt
     1261 cgaattcgtc ggtccgaaaa ttggcgagga tttgcgctgg cgcggcacgc tttccgtgat
     1321 cgccgccatg gtgatgatgg gtgtgtacat atgggttcgc ttcgcctcgg cgttcggtgc
     1381 gaagttcggt ctgcatttcg gcctgggcgc ggtgatcgct ttgatgcatg acgtgctggt
     1441 gaccgtcggc gccttgatgc tggccaacta cgaattcgat ctgaccgtgg tcgcggcatt
     1501 gctgaccatc gtcggttttt cggtcaacga tacggtggtt atctgcgatc ggatccggga
     1561 gaacttgcgt aagatccggc gcgaaagttt ggaaagtatc atcaatacca gcttaaacga
     1621 gactctgagc cggacgatac tgaccaccgg cacggcatta ttggtattgt tggcgctcta
     1681 cctgctcggc ggcgcggtga tccggccctt cgccttcgct cttttagtcg gttttgtctc
     1741 cggcgtctac tcgaccatct tcatcgccag cccggtcatc ctgctgtggg agaagtcgtt
     1801 taggagatag cggcgaccaa cgaagataaa gatagttttg accgggaaga cacgaagaca
     1861 acgaagttcg aacattagtt ttccgaaacc ttcgtgacct tcgtgacctt cgtgtcttcg
     1921 gggtgagatc ggattctctt ttcaatctcg taactctcca tgccgcatga tcttgccaag
     1981 cgttggcgct tgaaaccgag cgatgaggcg acggtcatgc gcttcgccgc agaacttaag
     2041 ctttcgccgc tgatagcgcg catcctgacc ttgcgcggct ttatcgacaa cgacgcgacc
     2101 cgctgctacc tgtcatcgag tttacgttcg gatttgccgt ctcccttcga gatggcagat
     2161 atggacgcgg cggtggagcg cattgccgct gcagtgacaa gcaatgaaca gatcgctatc
     2221 tggggcgatt acgatgtcga cggcaccact ggggcatcgg tgctggtgtc attcctgcgc
     2281 gaggtcggcg ctaagccgtt ctaccatgtg ccccatcgca tcgaagaggg atacggtctc
     2341 aacctcgaag gattaaaagc gctcaaagag cgcggcgccg gtttggtgat caccgtcgac
     2401 tgtggcatct ccaacgccaa agaagttgcc gccgccaatg gcttcggtct cgatctagtc
     2461 gtcgtcgatc atcatcaacc gcccgaagtg ctgccgccgg cggtggcggt ggtgaatccg
     2521 caccgcaagg attgccgttt tcccgacaaa ggcatgtgcg ccgccgggct ggccttctat
     2581 ttggtgatcg gcttgcgcgc caagttgcgt gaagcgggtt ggttcaagca cggcgccgcg
     2641 cccgatatcc gccatttcct cgacattgtt acgttgggaa ccatcgccga catggtgccg
     2701 ctcaggggcg tcaaccggac gttgatcaag cgcggcctta ccgagttggg gatttcgact
     2761 aggcccggcg tggttgcgct aaaaaaagtt gccgggattc ctgacggcga tgtcactgct
     2821 gggcaggtag gctttcgctt ggggccgcgc atcaatgccg cgggtagggt ggattacggc
     2881 atcaaagtcg tcgagctttt aaccaccgaa tccagcgaaa ttgcccagcg catcgcccag
     2941 gaacttgagg agcataatct cgaacggcgc gccgtcgaag cggaagtaca gcagcgtgcc
     3001 ctggcgcagg ccgaagcggc ggttgccgcc ggtagttgct attcactggt cctggccggc
     3061 gcaggctggc atccgggcgt gctcggaatc gtcgcgtcgc gggtggttga gcgcttctac
     3121 cgtccgacgg tggtgatcgg tttcgataac ggcgtcggca agggctcggc acggagtatc
     3181 cgtggcttca atattgtcga ggcgtttcac gtttgcgccg cacacttgga gaaattcggc
     3241 ggtcatgagt tcgccggcgg cttgtcgata cggccggaat gtctagagcc gtttgccgca
     3301 gctttcgaaa aggcggcgcg ccaaactctc agcaacgaac aactctcgcc gctgttgcaa
     3361 atcgacgcgc agttggattt ctcgcaactg actttgccgt tgatgcgtga attggaagtc
     3421 atgcagccct tcggtgtcgg caatccggaa ccgatgttca ccacagttaa cgcggaaatt
     3481 tgcgagcgca aagtgttttc cgccggtgtg cggttccgat tgcgccaggc cggcaaagta
     3541 gtcagcggcg tgattttcgg cgttggcgag gaatacccgg gacagcccgg tgagagcgtc
     3601 gacgtggcgt atcggctctc ggaaaatcaa tggaatggta acaccacggt cgagttgaag
     3661 atcatcgata tgcggatggc gaaaccgggg ttcgcggtta ttgttagagc ggccggggat
     3721 tagttcgggt atttcaaata agtggggacg gtgaatgaaa atgtttaaag cgctcctgtt
     3781 cagcactttt ttgatgcatg catccgtcca agtggggctc gccgccgagc ctggcggcaa
     3841 gccggagaag ccggatgtca tagtaaccta tgcacagccg agcggcgcgt tcacgcctat
     3901 ctgggtggcc tatgaagcgg ggttgttcaa aaagtatggc ttgaacgcca ctttgcagct
     3961 cttgaaccca caggtgtcgg cgcaagcggt gatcgccgag gaggcggatt tttacaccaa
     4021 cgggccggat ctgatcaacg cgcgattgca cggcggccag gtgaaatatt tcggcgggac
     4081 gattcagcag ctagtgtttc aaatctgggg cgccaaggaa atcaagacca tccaagatct
     4141 aaagggtaaa accgtggcgg cgagtacgcc gcgcgccgcg ctcgataccg ctacccgcga
     4201 agcgctcaag aaaaatggcc tggtaccgga caaggacgtg cagattcttt acgtgcagtc
     4261 ggtgccggcg atcctgagct cgatcctcgg cggtaaaacc gccgccggca cgctgtcggc
     4321 acccaatact gtcaaggcca aagagtcagg gctgaatttg ctggtcgaca tcggcaagtt
     4381 gaatatcccg gcgttccaag tgacctacgg cgccacggaa aaatatttga aaaataatcc
     4441 caacaccgtg tacgctttct tgaaagcgat cgccgaaggc gtggtgctat cgcgcaatga
     4501 cccggcggtg gcgaaaaagg cgatcggcaa atacgtcaag atcgacgacg cgccgaccat
     4561 cgatggcacg tacgacgcat tcgcgcccta ttgggcaatg agcctggcgg tgcggcccga
     4621 ggcgatccgc ggccagttcg agtacatcga tgaaaaagaa tttcccaagg tgaaaaacgc
     4681 cgatcccaaa gagttctacg acaactcctt cgtcgaagca ttaaccaagt caggttttct
     4741 gaaaagtctc ggcatgaagt agccgacaca gatcgctgac ccgacagcgg gccatgggaa
     4801 ttcaaagccg cagggatatt gtctacctgg cgctggccgg tttcttcgtc accaatgcga
     4861 tcctcggcga actcaccggc ggtaagcttt ttactctcgg tccatttact ctaagcatcg
     4921 gcgtgatccc ctggccaatc gtcttcatcg ctaccgatct gattaatgaa tacttcggcc
     4981 gtgaaggggt cag
//
LOCUS       DASQKS010000385         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3571752, whole genome shotgun sequence.
ACCESSION   DASQKS010000385 DASQKS010000000
VERSION     DASQKS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3571752"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VE170_09995"
     CDS             <1..1104
                     /locus_tag="VE170_09995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HYJ15812.1"
                     /translation="IYRHHVPKCPAEKNCVADFVTFYASGQIIWDDRQALYDLERQHA
                     YQNRIAPVERVLPFVYPPITAAFLAPLAWLQFPTAFLLLTTVNLLLIGQSLRLLMRHL
                     DLTIDQAHWLMLFTLCNFAVHGVIFYGQTSAIVLFFLTLHVLAQRKAERAVTGVWAGL
                     LCVKPQYLAVPHFILLVRRRWREFVVGAAVGSLLIIGMFLWIGLEASAQYFQLAGRMV
                     SADSDWWNQWRGMHNLRVLVIYGLPKAWQAAAWWSLSAIVVAILAWVNWPAAQHARDH
                     ASCWIVNCLGLLIVLPHLFTHDLTLLIVPCALLLSKVKPQVPLAMGIGLVMLAMLPAL
                     NSTVPAIMAIALVLLFATSLLLLTRGKMGWVGK"
     gene            complement(1114..2094)
                     /locus_tag="VE170_10000"
     CDS             complement(1114..2094)
                     /locus_tag="VE170_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15813.1"
                     /translation="MIQTIFATLILLMGAGVAVAQTPARVMTGYAATSGPHAVLWIAR
                     EAGLFDKNGIKAEVAYIRSGSTMAQTLVSGEIHLSQMGGPAMLSAGVAGMDVTFVAVA
                     LNTTPIVIMGNVAKMEDLKGKSLGVTRYGSNTDTSARYAIRKAGLQPEKDVALIQLEE
                     YANILSSIQTGRVAAGALADPFTYAAKKLGINELADIAAMGLEFPFVGIAAKKSYIKD
                     NPDTVQRFVRAYTEAIAIYKNNRELAMKVTSKYTGMKDPAVLSATVDFYAPKLQRVPH
                     ATVAGLKFVLDEVAVRDPRAKNYTPETFMDTRFVKQLEDSGFIKSLYPKG"
     gene            2197..3180
                     /locus_tag="VE170_10005"
     CDS             2197..3180
                     /locus_tag="VE170_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15814.1"
                     /translation="MTRIRRILALSAVTLAFWQTTAAVASNINVVWTSEVGQFAPLWV
                     TKEAKLFEKYGNNVELIFIQGASLAAAALTSGDAQVGMFSPQVVITNPGLELVMFGRL
                     GNTMDNRVYGKKGIKSIKEVKRIAISRFGSNADFAARFLLQREGLRPDTDVALLQFGN
                     QSNRIAAVETNNADAAMLTPPMTLQARKLGMTLLLDASKLNIPYSSLFFVSRRPYLTK
                     SRAELINFTKAMIEGVALYKSNKEFALKVLAKYLKVQDREILEENFREYDFPLKPSPS
                     REYFELPIQEVGKKDPKVLKENPERFTDLSLVKEIESSGFIDKVSQEYRVK"
     gene            3296..4390
                     /locus_tag="VE170_10010"
     CDS             3296..4390
                     /locus_tag="VE170_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYJ15815.1"
                     /translation="MASVRGATLLGPDKLELREYPMPEIPADGGLLKIEMGGVCGTDV
                     KYLHGKLKVDYPIILGHEILGRVEKLGKNAAAIHHVKEGDRVILKGALGCGRCADCRR
                     NNQRFCRQRTNYGGRTKSDLPPHLFGGFADYLYLAPDVLCTKVSESLSAEAAALVGSV
                     MANGFQWAIRRGGVKMGDYVLIQGPGQQGLSCTFASKHAGAAKIFVTGIGRDQPRLEL
                     AKKFGATRTINVEEEDVVEVIRKETNGELCDVVIDVSGNPNAILKSVDCLHRQSTMVL
                     GGLTGDTTVTPMLMDKLVWNEIKLQGCFTADNDATEAALRIIEETKFPVEQMVSHVFP
                     LKDIEHCIRSIGGEVPGTFPTKALINPALG"
     gene            4458..>4671
                     /locus_tag="VE170_10015"
     CDS             4458..>4671
                     /locus_tag="VE170_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15816.1"
                     /translation="MIEGLTDKVVIVTGGAHGIGKAYCNGFAGAGARVVIADIDLPGA
                     AAAATEIGAQALAMQVDVSNQEATKQM"
BASE COUNT          993 a         1317 c         1337 g         1024 t
ORIGIN      
        1 atctataggc atcatgtgcc caaatgtccc gccgaaaaaa actgtgtggc ggatttcgtc
       61 accttctacg cttccgggca aatcatctgg gacgatcgtc aagcgcttta cgatctcgag
      121 cgccagcacg cctatcaaaa ccgcatcgct cctgtggaaa gggtcttgcc tttcgtttac
      181 ccgccgatta ccgcggcgtt tctcgccccc ttggcatggt tacaattccc gaccgccttt
      241 ttactgctca cgacggtcaa cctactgctg attggccaga gtttacgatt gctcatgcgc
      301 cacttggatt tgaccatcga tcaagcgcat tggttgatgt tattcacctt gtgcaatttt
      361 gccgtgcacg gagtgatctt ctacggccaa acctcggcga ttgtgctgtt tttcctgacc
      421 cttcatgtac tcgcccaaag aaaagcggag cgcgccgtga cgggagtttg ggctggactg
      481 ttgtgcgtca aaccgcaata tttggcggtg cctcatttta ttctcctggt gcggcgccgc
      541 tggcgggagt ttgtcgtcgg cgccgccgtc ggcagcctgc tgattatcgg catgttccta
      601 tggatcgggc tggaagcctc ggcgcagtat tttcagctcg caggccggat ggtgagcgcc
      661 gatagcgact ggtggaatca atggcgtggc atgcacaacc tgagggtgtt ggtaatttac
      721 ggcttgccta aggcttggca ggccgcggcg tggtggagct tgagcgccat cgtcgtcgcc
      781 atcttagctt gggtcaattg gccggcggcg cagcatgcca gggatcacgc tagctgctgg
      841 atcgtcaatt gtcttggcct gctcattgtg ctgccgcatt tgttcaccca tgatcttacc
      901 ttgctaatcg tgccctgcgc gctactgctg agcaaggtca agccgcaagt gccgctcgcc
      961 atgggtatcg gtttggtcat gctggcgatg cttcccgcgc tcaactctac agtaccagcg
     1021 atcatggcca tcgccctggt gctgttattc gccaccagcc tgttgcttct gacccgaggg
     1081 aaaatgggct gggtcgggaa atagccggcg gccttacccc ttggggtaga gcgacttgat
     1141 aaagccgctg tcttccaact gtttgacgaa gcgcgtgtcc atgaaagtct ccggggtgta
     1201 gttcttggcg cgcggatcgc gcaccgcgac ttcgtccaag acaaatttca aacccgctac
     1261 cgtcgcatgc ggcacccgct ggagcttggg agcgtagaaa tccaccgtgg cgctgagcac
     1321 cgccgggtct ttcataccgg tatatttgct ggtgactttc atcgccagct cgcgattatt
     1381 cttataaatg gcgatcgctt cagtgtaggc gcgcacgaaa cgctgcaccg tgtccggatt
     1441 gtctttgata taggacttct tggcggcaat gccgacaaag ggaaattcca gccccatggc
     1501 ggcgatgtcg gcgagttcat ttatgccgag cttttttgcc gcgtaggtaa acggatcggc
     1561 gagggcgccg gcggctaccc ggccggtctg gatgctgcta agaatattgg cgtactcttc
     1621 aagctggatc agcgcgacat ccttttccgg ttgcaagccc gccttgcgga tggcatagcg
     1681 tgccgacgtg tcggtgttgg agccatagcg cgtgaccccc agcgatttgc ctttcaaatc
     1741 ttccatcttt gcgacattgc ccatgatcac gatcggcgtc gtgttgagcg ccaccgcgac
     1801 gaaggtgaca tccatgccgg cgaccccggc ggagagcatc gccggcccgc ccatctgcga
     1861 caggtgaatt tcccccgaca ccagggtttg cgccatcgtc gacccgctgc ggatgtaagc
     1921 gacttcggct ttgatgccgt tcttatcgaa cagtcctgcc tcgcgcgcga tccagagcac
     1981 cgcatgagga ccggaagtgg cggcataacc cgtcatcacc cgcgccggcg tctgcgcgac
     2041 ggcaacgccg gcgcccatca ataatattag cgtcgcaaaa atcgtttgga tcatttgtcc
     2101 tccactaact tgattccaag gtcggttcgg aagtataggg gtaagcgtaa accaaggtca
     2161 accgcagttg cggttcattc ttttaaggag aagtgaatga caaggattcg ccgaatctta
     2221 gcgctgagcg cggttacact cgcgttctgg caaacgacag cggctgtcgc ttcaaacatc
     2281 aatgtggttt ggaccagcga ggtcggccag ttcgcgccgc tttgggtgac caaggaagcc
     2341 aagctgttcg agaaatacgg caacaacgtt gagctaattt ttatccaagg cgcgagttta
     2401 gccgccgcgg ctttaacatc gggcgacgct caagtcggta tgttctcgcc ccaggtggtg
     2461 attaccaacc cggggttgga attagtcatg ttcggacgcc tcggcaatac catggacaat
     2521 cgggtgtatg gcaagaaggg catcaagagc atcaaggagg taaagcgcat cgccattagc
     2581 cgcttcggca gcaacgccga tttcgccgcc cgtttcttgc tgcagcgcga gggcctgcgg
     2641 cccgacaccg atgtcgcctt gctccaattt ggcaatcaat cgaaccgcat cgccgccgtc
     2701 gagaccaata acgccgacgc cgccatgctg acgccgccga tgactctgca agcgcgcaag
     2761 ctcggcatga cgttgctgct cgatgcgtca aaattgaata ttccctattc cagcctgttt
     2821 ttcgtgtcgc gccggcccta tctgacgaag tctcgggcgg agttgatcaa tttcaccaag
     2881 gcgatgatcg aaggcgtggc gctttataag tccaacaagg agttcgcctt gaaggtctta
     2941 gcgaaatacc tgaaagtgca agaccgcgag atcctcgaag agaacttccg cgaatacgat
     3001 tttccgctga aaccctcgcc atcccgcgaa tatttcgaac tgccgatcca ggaagtcggc
     3061 aagaaagatc ccaaggtctt aaaagagaac cccgagcgct tcaccgacct atcgttggtg
     3121 aaagagattg aaagcagcgg cttcatcgat aaagtgtccc aagaataccg ggtgaaatga
     3181 cttaacccct gattgttatc aggggttaag tcatcccgag cgacagcgag ggatctcgac
     3241 cgagaagatt tcgtccggaa agatttggat aatgaatatt taaggaggac aaaccatggc
     3301 aagcgtaaga ggcgcaaccc tactggggcc ggataaactg gaactgcgcg aatatcccat
     3361 gccggaaatt cccgcagatg gcggcttact taaaattgaa atgggcggtg tgtgcggcac
     3421 cgacgttaaa tatctccacg gcaagctcaa ggtcgactat ccgatcattc tcggccacga
     3481 gattcttggc cgggtcgaga agctcggcaa gaacgccgcg gcgatccacc atgtcaaaga
     3541 aggcgaccgg gttattctca aaggcgcgct gggctgcggc cgctgcgccg actgccggcg
     3601 caacaaccaa cgtttttgcc gccaacgcac caattatggc ggccgcacca aatccgacct
     3661 gccgccccat ctgttcggcg gctttgccga ttatctttat ttggcgcccg atgtgctttg
     3721 caccaaagtg agcgagtcgc tgtccgccga agcggcggcg ctggtcggct cggtcatggc
     3781 caacggtttt caatgggcga tccggcgcgg cggcgtcaag atgggtgatt acgtgctgat
     3841 ccaaggcccc ggccaacaag ggctgtcctg caccttcgcg tccaaacacg ccggcgctgc
     3901 gaaaatcttt gtcaccggca tcggccgcga ccagccgagg ttagaattgg cgaaaaaatt
     3961 cggcgccacc cgcacgatca atgtcgagga agaagatgtc gtcgaagtga tccgcaagga
     4021 aaccaacggg gagttgtgcg atgtcgtcat cgacgtgtca ggcaatccca acgcgatctt
     4081 gaagtcggtg gattgtctgc accgtcaatc gaccatggtg ctgggcggtt tgaccggcga
     4141 caccacggtg acgccgatgt tgatggataa gctagtgtgg aacgagatca aactgcaagg
     4201 ctgcttcacc gccgacaacg acgccaccga agccgcgttg cggatcatcg aggaaaccaa
     4261 gtttccggtc gaacagatgg tcagccatgt ctttcctttg aaagacatcg aacattgcat
     4321 tcgctccatc ggcggcgagg tgccgggcac ctttccgacc aaggcattga tcaatccggc
     4381 acttgggtag gcgataggcg acggcgcgat ccgtggtagg ggcgattcat gaatcgcccc
     4441 tacatttaat caatggaatg atcgaaggcc tgacagacaa agtcgtcatc gtcaccggtg
     4501 gcgcccatgg tatcggtaaa gcttattgta acggcttcgc cggcgccggc gcgcgcgtcg
     4561 tcatcgccga catagacctg ccaggcgcgg cagcggcggc cacggaaatc ggcgcacaag
     4621 ccttggcgat gcaggtcgat gtatcgaacc aggaagcgac caagcaaatg g
//
LOCUS       DASQKS010000386         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5653781, whole genome shotgun sequence.
ACCESSION   DASQKS010000386 DASQKS010000000
VERSION     DASQKS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5653781"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="tpiA"
                     /locus_tag="VE170_10020"
     CDS             complement(<1..560)
                     /gene="tpiA"
                     /locus_tag="VE170_10020"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYJ15817.1"
                     /translation="MSTPLVVANWKMHGDQARCASLARQVVRALRRPPVDAEVVLAPP
                     FTALNTVHRCLGGSPIKLAAQNCHWESQGAYTGEISPAMLNELGCAYIIVGHSERRQL
                     FHESDAIIARKLKSVLSQGMRAILCVGETLDERQSKNTVLVIRRQLRIALKGLEKTDI
                     ANIEIAYEPVWAIGTGQNATPKQISQV"
     gene            complement(557..1738)
                     /locus_tag="VE170_10025"
     CDS             complement(557..1738)
                     /locus_tag="VE170_10025"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYJ15818.1"
                     /translation="MIRILTELELNDKTVFLRVDFNVPLKDGKVGEPHRIDSALPTLR
                     FILDRAKKVIVASHLGRPDGKFVDKYSLAPVQRYLQDALQFPVAMASDCIGPSVENLL
                     RTGAGKVVLLENLRFHPEEEKNDENFSRALAALADTYVNDAFGAAHRAHASIAGMTKF
                     FKNKGAGLLLQRELDYLAPLLANPAQPFVTILGGAKVSDKIGVIRNLLPKVSALLIGG
                     GMAYTFLKAQGIEIGASLLEQDKIELAAELMQQAKQLGVNLLLPVDHIAGDRERKNPV
                     TINDSAVPRDLMGLDIGPKTAQVFAAEIERAKLVLWNGPVGLFEVPPYDQGSRVLAQA
                     LADNHPAVTGIIAGGDTVAAVSAAGLSSRIAHLSTGGGATLEFLEGKILPGIKALDDQ
                     P"
     gene            1841..2581
                     /gene="tolQ"
                     /locus_tag="VE170_10030"
     CDS             1841..2581
                     /gene="tolQ"
                     /locus_tag="VE170_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HYJ15819.1"
                     /translation="MSFFGPLDLIVQNALPAQHQHGILDLVRGSGLVVQGILYVLVFF
                     SVVSWGIIAQKFQQIRRAKRESAKFIEIFWERRNLSSIHDASRELASSPVGQVFRAGY
                     EELVRVSHSKKELSPGENLTTELGGVDNVSRAMQRATSVEITKLEKHCTFLATTASSA
                     PFVGLFGTVWGIMNAFLGLSVTQSSSIQAVAPGIAEALIATAVGLGAAIPALIAYNYF
                     VQQIKVVAVEMDNFSHEFLNIAERHFFK"
     gene            2641..3057
                     /gene="tolR"
                     /locus_tag="VE170_10035"
     CDS             2641..3057
                     /gene="tolR"
                     /locus_tag="VE170_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HYJ15820.1"
                     /translation="MDASSQRNGATIAQINVTPLVDVMLVLLVIFMVTAPIIQQGVQV
                     NLPAARSNAIPGTEEHLVVTVAKNGRIYLNDNAMTSGELGSKLRAIVKSQPNKQVYLR
                     ADQDVRYGLVMQTIAEIKQAGIERLGMVTRPQDKEG"
BASE COUNT          667 a          854 c          934 g          762 t
ORIGIN      
        1 acttgactga tttgttttgg cgtcgcgttt tgtccggtgc cgatggccca gaccggctcg
       61 taagcaattt cgatattggc tatatcagtt ttctccaatc ccttcaatgc gatccgcagc
      121 tggcggcgaa tcaccaggac agtgtttttg gattgacgtt catcgagagt ttcgccaacg
      181 cagagaattg ctcgcatccc ctggctcaac acggatttta gcttgcgcgc gattatcgcg
      241 tcactttcgt gaaaaagctg gcgtcgctcg gaatggccga caatgatata ggcgcaaccg
      301 agttcgttta gcatcgcggg gctgatctcg ccagtatacg cgccctggct ttcccagtgg
      361 caattctgcg cggcgagctt aattgggctg ccgccgaggc aacgatgaac cgtattcagc
      421 gcggtgaatg gcggtgccag aacgacctct gcgtcgaccg gtgggcggcg caacgcgcgg
      481 acgacctgcc gtgccaggct ggcgcagcgg gcctgatcgc cgtgcatctt ccaattggcg
      541 actaccagcg gtgtgctcat ggttggtcgt ccagcgcctt aattcccggt agtattttgc
      601 cttcaagaaa ttccaaagtc gcgccgccgc cggttgacaa atgggcgatg cgtgaactca
      661 gcccggctgc cgagacggcc gctaccgtgt cgccaccggc aatgatgccg gtcaccgcgg
      721 gatgattgtc ggcgagggct tgcgcgagta cgcgactccc ttggtcgtag ggtggcacct
      781 cgaagagccc caccggcccg ttccatagaa ccagcttggc cctttcgatc tcggcggcga
      841 agacttgcgc ggtcttaggg ccgatgtcga gtcccatgag gtcgcgtgga accgcgctgt
      901 cattgattgt tacaggattt ttcctttcgc ggtcgccggc gatatgatcg accggcagta
      961 gcaggttgac accgagctgc ttggcctgct gcatcagttc ggctgcgagt tcgatcttat
     1021 cctgctccaa aagcgacgcg ccaatttcga tgccctgggc ctttaggaag gtataggcca
     1081 tgccgccgcc gatcagaaga gcgctcactt tgggcagaag atttcttatc acaccgattt
     1141 tgtcggaaac ctttgccccg cccaggatcg taacgaaggg ctgcgctgga ttggccagta
     1201 gaggcgcgag gtagtccaac tccctctgca agagcagtcc ggcgccttta ttcttgaaga
     1261 atttagtcat gccggcaatc gaggcgtggg cacggtgggc cgctccgaag gcgtcgttaa
     1321 cataggtgtc ggcgagggcg gccagcgcgc gggaaaaatt ctcgtcgttt ttttcttctt
     1381 ccgggtgaaa acggaggttc tccaggagta cgactttgcc tgcgcccgtg cgcagcagat
     1441 tttcgaccga cgggccgatg cagtcgctgg ccatggcgac gggaaattgc agggcatcct
     1501 ggagataacg ttgcaccggc gccaggctgt atttgtcgac gaatttaccg tcaggtctgc
     1561 ctaggtgcga agcgacaata actttcttgg cgcgatccaa aatgaaacgg agggtcggta
     1621 gcgcgctgtc gatgcgatgc ggctcgccca ctttgccgtc tttgagcggc acgttaaagt
     1681 cgacgcgcag aaaaaccgtc ttgtcattca gctcgagttc ggtaagtatt cgaatcatag
     1741 ttcgatctcc gcccaggaaa ttctagttaa aagcgctttg tcaattgact aattacaagg
     1801 cttgtaatat tttttcaatt atccgcagca ataacccgtg atgtcttttt ttgggccgct
     1861 cgatctaatc gtacaaaacg ctctgccagc gcagcaccag cacggaattc tcgatctggt
     1921 tcgtggctcc ggactggtcg tgcaggggat actctatgtc ctggtttttt tctcggtcgt
     1981 gagttggggc attatcgctc agaagttcca gcagattcga cgcgccaaga gggagtcggc
     2041 gaagtttatc gaaatatttt gggaacggcg caatctttcg tcgatccacg atgccagccg
     2101 tgaattagca tccagtccag tggggcaagt gtttcgtgct ggttacgaag agctggtgcg
     2161 ggtatcacac tcaaagaagg aattatcgcc cggggaaaat ctcaccaccg agttgggcgg
     2221 ggtcgacaat gtctcgcggg cgatgcagcg ggcgaccagt gttgagatca cgaagctgga
     2281 aaagcactgc acattcctgg ccacaacggc aagttcggcg ccgttcgtcg gactgttcgg
     2341 tacggtttgg ggcatcatga atgcttttct cgggctcagc gtcacccagt cttcgagtat
     2401 ccaagctgta gctccgggca tcgccgaggc gttgatcgcc accgcggtcg ggttgggtgc
     2461 ggcgatccca gccttaatcg cctacaatta ttttgttcag cagatcaagg tcgtcgccgt
     2521 cgagatggat aacttttctc acgaatttct caatatcgcc gagcgtcact tcttcaagta
     2581 accaaataaa agcgtataat cagagtatcg ccggtagata actgaggaga cggtctagct
     2641 atggatgcat cgtcccagcg caatggcgca acaatcgcgc agatcaacgt cacgcctctg
     2701 gtcgatgtaa tgctggtttt gctggtgatt tttatggtga cggcgccgat catccagcag
     2761 ggcgtccagg tcaacttgcc ggcggcgcga agcaatgcca tacccggcac cgaggagcat
     2821 ctggtggtga ccgtggccaa gaacggtagg atctatttaa acgacaatgc catgacctcc
     2881 ggcgaactcg ggtcgaaact gcgggcgatt gtgaagtccc agccgaacaa gcaggtctat
     2941 ttaagggccg atcaagatgt ccgctacggc ttggtgatgc aaactattgc tgaaatcaaa
     3001 caggccggca tagaaaggct gggaatggtc acccggccgc aggacaaaga gggctgattg
     3061 tcgatggccg gagataattc ctctccaacc attagcttgc gtgaacgttc gtttcctctt
     3121 gccggcgccg aagaacgact gccgagatgg gtgacgtttt ccctgatcgt ccatgttggt
     3181 ttggcactat tgattattat ttcaccgttt acatcgt
//
LOCUS       DASQKS010000387         6888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3149479, whole genome shotgun sequence.
ACCESSION   DASQKS010000387 DASQKS010000000
VERSION     DASQKS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149479"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1257)
                     /locus_tag="VE170_10040"
     CDS             complement(265..1257)
                     /locus_tag="VE170_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15821.1"
                     /translation="MAKLKRFATLVPLFLTLLCSWVNAALSADSSEKLTRIRMGLAAR
                     STTSMPFFVAKERGFFREEGLEVELIVMQAIQTIQATLGNSTQFASATGSAVSAAVRG
                     ADIKVILAVTDRPSFDLIVQPNITSVQQLRGKKIGTGGVGSLAEILTRRILLANNVRP
                     EEVAILATGPSHVTYLSLKAKVIDAAPLQMPLTFTAQDEGFRKLVAAGDVYQTVQGGL
                     ATTTTMLTEQPEVVTKVVRAMLRATRLFKSDRKYGIEFLKGPWLDIGKDPEKIAARTY
                     DIAAPALLENGIVGEDIQRQMIADAAAQIKPKQPVLPAQVFDFSIARKVGEALR"
     gene            complement(1385..2002)
                     /locus_tag="VE170_10045"
     CDS             complement(1385..2002)
                     /locus_tag="VE170_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1958..1960),aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HYJ15822.1"
                     /translation="MSEPLVLEIFSDFVUPWCYLVTGRVEKLKNQYGLGIKFTNFPLH
                     PETPEEGSVRGPEIIARNGRMKVNMDREGLPFNAERNMSFNSRLAQELATWAATKGKA
                     EEVVNALFRAYFVDVKNIGKVDVLAQIAEQNDLPKDEATEILLSRKYKDAVDTDWKRC
                     ASFGINGVPTFLAGKYLMVGAQPYEELQRLIEHVLKEPAAHSSDG"
     gene            2211..2936
                     /gene="pcaD"
                     /locus_tag="VE170_10050"
     CDS             2211..2936
                     /gene="pcaD"
                     /locus_tag="VE170_10050"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HYJ15823.1"
                     /translation="MVFSNSLGSDLRIWDGVAARLLQHCHVVRYDLRGHGLSEAPTPP
                     YSAADLARDVVALLDRLEIRGAVICGVSVGGMIAQAVALNHPERVRALVLSDTGARIG
                     SAAAWQQRIDKVRADGVDSLVELTMERWFSAGFREKWPADIRGYSLMLRQCSADGYIG
                     TCAALRDADFRSTAVQIRQPTLVLCGAEDIATPPELGRELASLIPGAQFSLIEKAAHL
                     PCVEQPDAMFERMIQFFREVDIV"
     gene            2929..3315
                     /gene="pcaC"
                     /locus_tag="VE170_10055"
     CDS             2929..3315
                     /gene="pcaC"
                     /locus_tag="VE170_10055"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HYJ15824.1"
                     /translation="MSDELFDKGMAVRKAVLGEEYVARAEANKTDFDADWQRYVTEHA
                     WAAVWTRAGLEKRTRSMLTIAMLASLGRLDELGAHIRATRNTGVTKDEVKEVLLQVAV
                     YAGAPAANSAFAIAKRIYKEMEMEGK"
     gene            3434..4143
                     /gene="pcaH"
                     /locus_tag="VE170_10060"
                     /pseudo
     CDS             3434..4143
                     /gene="pcaH"
                     /locus_tag="VE170_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
     gene            4140..4712
                     /gene="pcaG"
                     /locus_tag="VE170_10065"
     CDS             4140..4712
                     /gene="pcaG"
                     /locus_tag="VE170_10065"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HYJ15825.1"
                     /translation="MSKQTPSQTVGPFYAIGLTRQPMNVVAWESTQGQRIRIEGQVCD
                     GDGVAIPDVMVEIWQANSYGRYNHPDDKQEKPLDPAFSGWGRSGTDEKCFYSFETIKP
                     GPVPGNDDTVQAPHVNVVIFARGMLVHAYTRIYFDDEAANANDPVLLSIKNKARRRTL
                     IAQRGERDGNAVYRFDIRLQGGNETVFFDM"
     gene            4820..6160
                     /gene="pcaB"
                     /locus_tag="VE170_10070"
     CDS             4820..6160
                     /gene="pcaB"
                     /locus_tag="VE170_10070"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HYJ15826.1"
                     /translation="MSLLDSTIFAPLFGDAAIDDLFSDQAYVHALVDIEIALARAGAR
                     VGVIPTGAAEQIAKASADGIDVSALTKGTLRSGFPIIALVQELRKQVGGNAAPYIHWG
                     ATTQDIMDTACVLQLREAIQLCSKTIGELTGHLTALAGKHRTTVLAGRTHSQQALPIT
                     FGLKAAGWLAPLIRHVDRLAEIEPRLLVVQFGGAAGTLAALGDQGLTVMQALANELKL
                     GTPAMPWHAQRDSFVEFGGWLSLVSGSLAKIAQDVILMAQTEVGEVGESAEEGRGGSS
                     TMPQKSNPITSELIVAAARMNASLLSALHNAQIQEHERGTHGWQVEWLALPQMVTLTG
                     GALKHAVYLAKNLQVDAAAMRANISRANDVILAEAAVFALAQALPRPQAEELVKKACA
                     VAMAENKSLIDIVKQLAGDSAVAINWQALARPENYLGESDRLIDRVLELARKRF"
     gene            6163..>6888
                     /locus_tag="VE170_10075"
     CDS             6163..>6888
                     /locus_tag="VE170_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HYJ15827.1"
                     /translation="MEHAPPTTLTELLSSRAQRHPDKLALIFKDRRWSYGELQREAER
                     VANGLLRMGVEKGERVAFFLPNCGEFLFSVFAVTQIGAVFVPLNPQYIAEEAEYVLQH
                     SEASIVLTSPELLPLIESVRGKLANLKQVIVTGAGEFAGALSWDKFLAGVKTGAPRVP
                     LDPEELASITYTSGTTDRPKGVMLSQYAYAFAPRKRAAGLGWNDHDRVLCVLPLFHVN
                     ALCHTCIAMLSVGGSIVLTEKFSA"
BASE COUNT         1391 a         1899 c         2077 g         1521 t
ORIGIN      
        1 gcctgtcgcg gcagaaaata cttgggctgg cggtggggtt ggtggcggtg gcgttgttga
       61 atcggtaaga cccaacagcg ccgccgtttc agcgagtcga gattggaact gcgctaccgt
      121 cctgggcggc aggtcccttc aacataggct ctatcagccc gctgtaattc atccttcgga
      181 acaacaaacc aaagaacaaa tagtggtgtg gttaggcgtc tcatcggatc tgttagttcg
      241 cctgatcgct gtggccgatg tatcctatct caatgcttca ccgaccttgc gggcgatgct
      301 aaagtcgaac acttgcgccg gcagcaccgg ttgcttaggt ttaatttgcg ccgccgcatc
      361 ggcgatcatt tggcgttgaa tgtcttcgcc gacgataccg ttttccaaca gcgccggagc
      421 ggcgatgtcg taggtgcgcg cggcgatttt ttccgggtcc ttgccaatgt ccaaccacgg
      481 acctttgagg aattcgatgc catacttgcg atcggatttg aagagccgag tcgctcgcaa
      541 catggcgcgc acgactttgg tcacgacctc gggttgctcc gtgagcatag ttgtggtcgt
      601 cgccagaccg ccttggaccg tttgatatac gtcgccggcg gcgaccagct tacggaagcc
      661 ttcgtcctgg gcggtaaagg ttaacggcat ttgcagcggc gcggcatcga tgactttggc
      721 ttttagcgac aagtaagtaa cgtggctggg acccgttgcc aggatagcaa cctcctctgg
      781 gcggacatta ttggctaata gaatccggcg cgtgaggatt tccgccaaag aaccgacacc
      841 gccggtgccg atttttttgc cgcgcaattg ttgaacgctg gtgatattgg gctggacgat
      901 aagatcgaat gacggccggt cggttacggc caatatcacc ttgatgtcgg cgcctcgcac
      961 cgcggcgctc accgccgacc cggtggccga ggcgaattgc gtgctattgc ctaacgttgc
     1021 ttgaatggtt tggatcgcct gcatgacgat caactcgacc tccaaacctt cttcgcggaa
     1081 gaagccgcgt tccttggcca cgaagaaggg catggaagtc gtgcttcgcg cggccaggcc
     1141 catgcggatt cgtgtaagtt tttcgctgct atcggctgat agggccgcat tgacccaaga
     1201 gcagagtaac gtaaggaaga gtggaacgag agtggcgaat cgttttagct tggccatcgc
     1261 gtccttcttg cgcttgcgtc catcccttca gccaatgaat ttcgatcgac ggatcaataa
     1321 attctgcggg gctgcctaca gcgccgctcc cggcccattt ctcgcgtgaa agttggcaaa
     1381 ccgactagcc atccgagcta tgggctgccg gctccttcaa gacatgctcg atcaaccgct
     1441 gcaattcctc atagggctgg gcgccgacca tcaaatattt gccggccaga aaggtcggca
     1501 cgccattgat gccaaaggag gcgcagcgct tccagtccgt gtcgacggcg tctttgtatt
     1561 tgcggctgag gagtatttcg gtggcttcgt ctttcggcaa gtcattctgc tcggcgatct
     1621 gcgccagcac gtcgaccttg ccgatgtttt tcacgtcgac gaagtaggcg cggaacagcg
     1681 cattgacgac ttcctccgct ttgcctttgg tcgcggccca ggtggcgagt tcttgagcga
     1741 gacggctgtt gaaggacatg ttgcgttcgg cgttgaacgg caagccttcg cgatccatgt
     1801 tcactttcat ccggccgttg cgcgcgatga tctcggggcc gcgcacgctg ccttcttccg
     1861 gcgtctcagg gtggagcgga aagttggtga atttaattcc cagaccgtat tgattcttga
     1921 gtttctcgac acgcccggtg acgaggtagc accacggtca aacgaagtcg gaaaagattt
     1981 ccaataccaa gggttcagac ataaatacct ccgtttaaat cgttcaaaag ttcaaggttc
     2041 aagggttaaa atggattcta gggttgttga ttggcggaag tcaaagactt tctcgccggt
     2101 taatttgaca ttgttgcgct ggctcggata gtttcacagc gatgccattc ttgcgaatcg
     2161 gggactctgt tcatcactat caaacgcacg ggccgaaaga aaaacccgcg gtggtttttt
     2221 ccaactcgct cggcagcgac ctgcgcatct gggacggggt ggcggcgcgc ttgttgcagc
     2281 attgtcacgt ggttcgttac gacttacgcg gccatggttt gtcggaagct cctacgccac
     2341 cgtattcggc tgcggattta gcgcgcgacg tagtcgcgtt gctcgatagg ttggaaattc
     2401 gcggcgcggt gatctgcggc gtgtcggttg gcggtatgat cgctcaggcg gtggctttga
     2461 accatcccga gcgcgtgcgc gcgctggtgc tatccgatac cggcgcgcgc attggcagcg
     2521 cggcggcgtg gcagcagcgt atcgacaagg tgcgcgccga tggcgtcgat tcgctagtcg
     2581 agttgaccat ggagcggtgg ttttccgccg gcttccgtga aaaatggccg gccgatattc
     2641 gcggctattc tctaatgttg cggcaatgtt cggcggacgg ctacatagga acttgcgctg
     2701 cgctgcggga tgcggatttt cgctcgaccg cggtacagat caggcagccg actctggttc
     2761 tctgcggcgc cgaagatatt gccacgccgc cggaacttgg tcgagagttg gcgagtctga
     2821 tccctggagc tcagttttct ctgattgaaa aggcggctca tctgccctgc gtcgaacagc
     2881 cggatgcgat gttcgagcgc atgatacaat tttttcggga ggtggacatt gtctgacgaa
     2941 ctcttcgaca aagggatggc cgtgcgcaaa gcggtattgg gcgaagaata cgtcgcgcgc
     3001 gcggaggcga acaaaaccga cttcgatgct gactggcagc gttatgtcac cgaacacgcc
     3061 tgggccgcgg tatggacccg agcggggctg gaaaagcgca cgcgcagcat gctgacgatt
     3121 gccatgctcg cttctctggg acgtctcgat gaactcggcg cgcatatccg ggcgacgcgc
     3181 aataccgggg tgaccaagga cgaggtcaag gaagtgttac tgcaagtcgc cgtttacgcc
     3241 ggcgcgccgg cggctaactc ggcatttgcc atcgccaagc ggatctacaa agaaatggaa
     3301 atggaaggaa aataagacta ttcaccacga aggcagcgag gaccacgaag ttcggtgaaa
     3361 ttattattcg aaaccttcgt gtccttcgcg atcttcgtgg cgaaaccatt tgtgcttgag
     3421 gccggctgtt atgatgaaca tcaaacccta caatccacgc gactggcgct cccatccgcc
     3481 ttacagtttc catgcctatg gttcgtcggt caagcgcgca ccgctcaaga agctcatgcc
     3541 gatagatcaa actctatcgg agatcaccgg cccgttgttc aacgatatca aactaacgcc
     3601 cggggaagac gacctagccc gcaatgccga cggcaaggaa gcggtcggcg agcgcatcat
     3661 cgtcgtcggc cgagtgttgg atgaggatga taagcccgtc cccaatacgt tgatcgagat
     3721 ttggcaggcc aacgccgctg gtcgctataa tcatccggtt gaccaacaca acgcgccgct
     3781 cgatccgaac ttcctcggcg cgggccgctg tatgaccaat gacaagggcg agtatcgtta
     3841 cttgaccatc aaaccgggcg cttatccgtg gctgaatcat acgaatgcct ggcgtccggc
     3901 gcatattcac atctcgctgt tcgggcctag cttgtgacta ggctggtgac gcagtttttt
     3961 ttccccggcg atcccttgat cccgcacgat ccgattctca attcagttcc gacacggagt
     4021 ggccgtgagc ggctgatggc gagctatgcg catgatgtaa ccgagcctga gttcgccttg
     4081 ggttatcgct tcgacatcgt gctgcgcgga cgcaaagcca cgcccttcga ggggccgcaa
     4141 tgagcaagca aacaccttcg caaacggtcg gcccgttcta cgccatcggt ctgacccgcc
     4201 aaccgatgaa cgtagtggca tgggagtcga ctcaaggcca gcgcatccgc atcgaagggc
     4261 aagtctgcga cggcgacggc gtcgccattc ccgatgtcat ggtggagatc tggcaagcga
     4321 actcctacgg tcgttacaat cacccggatg acaagcagga aaaaccgctc gatccggcgt
     4381 tcagcggttg gggtcgttcc ggcaccgacg aaaaatgttt ttacagtttt gaaaccatca
     4441 agccgggacc agttcctgga aacgacgaca cagtccaagc gccccatgtc aacgtcgtga
     4501 tttttgcccg cggcatgctg gtgcacgctt acacgcggat ttactttgac gatgaagccg
     4561 cgaatgccaa tgatccggtg ctgttatcga tcaaaaacaa ggcgcggcgt cggacgttga
     4621 tcgcgcagcg cggcgagcgg gacggtaatg ccgtttaccg tttcgacatc cggctgcagg
     4681 gaggcaatga aacggttttc ttcgatatgt gaggttgtaa ttcgagtgaa gccattggtt
     4741 cgagttgtcc aactttcctc cctttaccgg aagaagtttt taatggcttt cggaaaatga
     4801 aaaatttctc aagctaccga tgagcttact tgattcgact atttttgcgc ccttgttcgg
     4861 cgatgcggca attgacgacc ttttcagcga tcaagcctat gtccacgcgc tggtcgacat
     4921 tgaaatcgct ctcgcccgcg ccggcgcgcg tgttggtgtc attccaaccg gcgcggcgga
     4981 acagatcgcc aaagcaagcg ccgatggtat cgatgtctct gcgctgacga agggaacgct
     5041 gcgttcaggg tttccgatca tcgctttggt gcaggagttg cgtaaacagg tgggcggcaa
     5101 tgcggcgccg tacattcatt ggggcgcgac gacgcaggat atcatggaca cggcttgcgt
     5161 gttgcaactg cgtgaggcga ttcaactatg cagcaaaaca atcggcgagc tcaccggcca
     5221 cctgacggcg ctagccggca aacatcggac caccgttctc gccgggcgca cccatagcca
     5281 gcaggcgttg ccgatcacat tcggtttgaa agcggccggc tggttggctc cgctcatccg
     5341 ccatgtggac cgattggcgg aaatcgaacc tcggttgctg gtggtgcaat tcggcggcgc
     5401 ggcgggaacc ttggcggcgc ttggtgatca ggggttgacg gtcatgcaag cactggccaa
     5461 cgagttgaag cttggtacgc cggcgatgcc atggcatgca cagcgcgata gtttcgtcga
     5521 atttggcggc tggctcagtt tggtttcagg ttctcttgcc aagattgccc aggatgtcat
     5581 cctcatggcg cagaccgaag tgggcgaggt cggcgagtcg gccgaggaag gtcgcggcgg
     5641 cagcagcacc atgccgcaga agtccaatcc aatcaccagc gagttgatcg ttgccgcggc
     5701 gcgcatgaac gcgtcgctgc tctcggcgct gcacaacgcg cagattcagg aacatgagcg
     5761 cggcacccat ggctggcagg tggaatggct ggcgctgccg cagatggtca ccttgaccgg
     5821 cggcgcttta aaacatgcgg tttatctagc gaagaatttg caagtcgacg ccgccgcgat
     5881 gcgggcaaat atttcccgcg ccaacgatgt gattctcgcc gaagcggcgg tgttcgcctt
     5941 ggcccaagcg ttgccgcggc cgcaggcgga ggaattggtc aaaaaggcat gcgctgtcgc
     6001 catggcggaa aacaaatcgc tcatcgatat cgtcaagcag ctcgccggcg attcggcggt
     6061 tgcgatcaac tggcaagcgt tggcgcggcc ggaaaattat ctcggtgaga gcgatagatt
     6121 aatcgaccgc gtattggagc tggcaagaaa gcgtttttga ccatggaaca tgccccaccg
     6181 accacgttga ccgaattact gagcagccgg gcacagcgcc atcccgacaa actcgcgctg
     6241 atctttaaag accggcgctg gagctacggc gaattgcaac gtgaggcaga gcgagttgcc
     6301 aacggtctgt tgcgcatggg cgttgagaag ggcgagcgcg tcgcgttctt tctgcccaac
     6361 tgcggcgagt ttctgttttc cgtctttgcc gtcacccaga tcggcgcggt tttcgtgccg
     6421 ctcaatccgc agtacatcgc cgaagaggcc gagtatgtgt tgcagcactc cgaggcgtcc
     6481 atcgtgctca cctcgcctga gctgttgccg ttgatcgagt cggtgcgcgg caaactcgcc
     6541 aatctcaagc aagtgatcgt caccggcgcg ggtgagttcg ccggcgcgct atcgtgggac
     6601 aagtttttag ccggtgtaaa aaccggggcg ccgcgggttc cccttgatcc ggaagagctg
     6661 gcgtcgatca cctacacctc cggtaccacg gaccggccca agggtgtgat gttgtcacaa
     6721 tatgcttacg ccttcgcgcc gcgtaagcgc gcggcggggc tgggttggaa cgaccacgat
     6781 cgcgtgctct gtgtgctgcc gctgtttcat gtcaacgcgc tgtgccacac ctgtatcgcc
     6841 atgctgtcgg tgggcggcag catcgtcctg acggaaaaat ttagcgcg
//
LOCUS       DASQKS010000388         5547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3572477, whole genome shotgun sequence.
ACCESSION   DASQKS010000388 DASQKS010000000
VERSION     DASQKS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3572477"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..216
                     /locus_tag="VE170_10080"
     CDS             31..216
                     /locus_tag="VE170_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYJ15828.1"
                     /translation="MARSAALKHEPRFAAAAIEKVRLGTRVTVLSKERDWFKVKVKPS
                     GNVGYLRKEYLAAANIR"
     gene            complement(298..2265)
                     /locus_tag="VE170_10085"
     CDS             complement(298..2265)
                     /locus_tag="VE170_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HYJ15829.1"
                     /translation="MEIEESVRGRELSGFVGGLGRLLLVAIPLTGIFFLLNVPQYLGW
                     LVFNEQYMGLFLGLGLSATYILIPVKPSTGRNVVPWYDIIAALAGFAVGLYIFIFYPS
                     IVNSLGDIATERVILGCMTILLLAEASRRLVGWPLVIIASCFLLYAYFAYIFPGDFYG
                     KGWSINRLATYLYLDANGIFGQALQVGVSIVIVFVIFGEVLYVVGGAGFLSDFSLSLM
                     GRYRGGQAKIAIVSSSLFGNISGSAVANVVVDGAFTIPMMKKAGYPAPQAAAIEATAS
                     TGGQIMPPVMGAAAFLIAEYLQIPYAQVALAAAVPAVLYYVTLFIQVDLLAARNGIHG
                     LPREEVPHLLPVLKRSASFVLPLTVLIVWMFILNRRPEEAGLLAALAALIVGFLTPGI
                     KVGQAEILRILTNAGRGMLEIVAITGLAGVVIGVLQLTGLGFTLTLTLLNIGQSNALL
                     LLVLTALVSIILGMGMPTTAVYVLLAVLVAPGLAKLGIIPIAAHLFIFYFGMLSMVTP
                     PVCMASYAAASIGKTDPVQTGWEAMKLCSIAYIVPFLFVFSPSLLLIGHWYEVVLSVV
                     TAIVGSILLGVGMVGYLFRPVAGLKRLLFAIAAVGLLIPVVQTGKFASLTWASNGIGL
                     VLAVLLVGVEWLARGHAKTVVAPGGVKSPAR"
     gene            complement(2360..3361)
                     /locus_tag="VE170_10090"
     CDS             complement(2360..3361)
                     /locus_tag="VE170_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ15830.1"
                     /translation="MLALLLVAAPVQIADAKLDCGPTANKSIQLPRTIAIGTNPAGTG
                     AHAIASGLAAIASKTTQVSAKVQPYNGPNAWMPLLESGEIEFGIINILDSNMAATGTG
                     NYKKAYPGVRIVAGGVFPFTAGLIVKDKSDIKQMADLKGKRMAWDYGGHAINQTWQLA
                     VMEASGLKPADVQQVRVSNLNDGVRAVPEGKVDAAFTAVGIGVVEEANAMEPVRFLSM
                     AYTPAANKILGRYGASVVKSEAAAGVKGETYVIGYPLHLASMINVSDKTVYTMLKAWW
                     DNLPELQTIHPLFKKWPKDMQALTNFTVPYHSGAIQFYKEMKVWGAKQEARTKEICG"
     gene            complement(3431..4474)
                     /locus_tag="VE170_10095"
     CDS             complement(3431..4474)
                     /locus_tag="VE170_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ15831.1"
                     /translation="MLRVKNFRVISVVMAFMLSPLGNFRGSAEAKIDCSPTVEKSNAL
                     PRTVAIASNLAGTGAHALASGLSAVASKVTTMSAKVQPYNGPNAWMPLLEQGEIEFGI
                     INILDANMAATGSGNYKKAYPTIRIVSGGVFPFTGSIMVRDKSEIKSAADLKGKRMAW
                     DFGGHAITQTWQQAAMEVLGLKASDVQQVRFSNLNDAIRAVPEGKVDATFAAIGIGIN
                     EEANAMEPIRFLNLPDNAGSNKILAKYGAIIVKQDPTAGIRSDTRVIGYPLHLASSTK
                     VSDKTVATLLKAWWDNLAELQTIHPQFKKWTKDVQAITNFTVPYHDGAVKFYKEASVW
                     GAKHDARTKEICG"
     gene            complement(4512..>5547)
                     /locus_tag="VE170_10100"
     CDS             complement(4512..>5547)
                     /locus_tag="VE170_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ15832.1"
                     /translation="HSKQLIGAATVVTLLVAQTFVVGSANAKVDCGPVINKGNELARS
                     VAIGTSPAGTGAFSLGSGLAAVASKATPIIAKVQPYAGPNTFVPLLESGELEFGIINI
                     LDAHMAMTGTGTYKKAYPSLRVLTGGVFPFTGGIMVRDKSDIKQISDLKGKRLAWDFG
                     GHAVIQALLNAALETGGIKPTDITQVRLTTSNDGIRGVAEGKVDGTYASLGTGINEEA
                     NAMEPVRFLSIPHSEANGKILAKLGASVVKAEAATGVRGDTNIIGYPLHLLTSTKVNE
                     RTVTAMLKAWWDNHAELQTIHPLFKQFTKDVQAITNFTIPYHPGAVKFYKEAGMWGAK
                     HDARTREICG"
BASE COUNT         1247 a         1665 c         1489 g         1146 t
ORIGIN      
        1 tgaactaccg ttctcgatcg tgaaactgaa atcgcccgta gcgctgcgct caaacacgaa
       61 cctcgcttcg ccgccgccgc aattgaaaaa gtccgcctgg gaacccgcgt gacggtgctc
      121 agcaaagagc gcgactggtt caaggttaaa gtgaaacctt caggcaacgt cggttacctg
      181 cgtaaggagt atctggcggc cgccaatatt cgctaaccca ttgcgccgaa ttcaacctgg
      241 tcgacgcgat ggactctcca cggcttagtt cgcatagaga ccataacgtt gaaccgtcta
      301 ccgtgccggt gatttcacac cgcccggcgc gacaaccgtc ttagcgtggc cgcgggccaa
      361 ccactcgacc ccgaccaata agacggcgag caccaaaccg ataccattgg acgcccaggt
      421 caaactggcg aacttgccag tctggacgac cggaatgagc aagcccaccg cagcaatggc
      481 gaacaagagc cgcttaagcc cggcaaccgg gcgaaacaaa tagccaacca tgccgacgcc
      541 aagaagtatc gagccgacga tcgcggtaac caccgaaagc accacctcgt accagtggcc
      601 gatcagcaaa agggatggcg agaagacaaa taggaaggga acaatgtagg cgatactaca
      661 aagcttcatc gcctcccagc cggtttgcac cggatcagtc ttaccgatcg aggcggcggc
      721 atagctcgcc atgcacacag gcggcgtcac catgctgagc atgccaaagt agaaaataaa
      781 caaatgcgcg gcgataggga taataccgag cttcgctagg cccggcgcca ccaaaacggc
      841 gagcagaaca tagacagccg tcgtcggcat acccatgcca aggataatac tgacaagggc
      901 ggtgagcacc agcaggagca acgcgttgct ctggccgatg ttgagcaggg ttagcgtcag
      961 cgtgaatccc agcccggtga gttgcaaaac tccgatgacc acgccagcaa ggccggtaat
     1021 ggcgacaatc tcaagcatgc cacggccagc attggtgagg attctaagaa tctctgcctg
     1081 accaacctta atcccaggag tgagaaaacc gacgatgaga gcggcgagcg cggcaagcag
     1141 gccggcttct tccggtcggc gattgaggat aaacatccag acaatcagca ccgtcagcgg
     1201 caaaacgaag ctcgccgaac gtttgagcac gggcagcaga tgcggcactt cttcccgcgg
     1261 caggccgtgg ataccgttgc gtgccgccaa gagatcgacc tggatgaata gcgtcacgta
     1321 atacagtacg gctggcaccg ccgcggcgag cgccacttgg gcgtagggaa tttgcaaata
     1381 ctccgcgatc aaaaacgccg cggcgcccat taccggcggc atgatctgtc cgccggtgga
     1441 agcggtcgct tcgatagccg cggcctgcgg cgccggatag ccggcttttt tcatcatcgg
     1501 aatggtaaac gcgccgtcga ccaccacatt ggccaccgcg ctgccgctaa tgttgccgaa
     1561 caggctcgac gagacaatgg cgatcttcgc ctggccacca cggtagcgcc ccattaagct
     1621 caacgagaaa tcgctcagaa agccggcccc gccgacgacg tagagcactt cgccgaagat
     1681 gacgaagaca atgacgatgc tgacgcccac ttgcagcgcc tgcccgaaaa tcccattggc
     1741 atcgagataa agataggtcg ccagccgatt gatcgaccac cccttgccat aaaaatcgcc
     1801 tggaaagata taggcgaaat aagcgtatag cagaaaacaa ctagcaataa tcaccaacgg
     1861 ccaacccacc agccgccgcg acgcttcggc gagcaagaga attgtcatgc agccaaggat
     1921 cacccgctct gtggcgatat cgccgaggga attcacgatt gacggataga aaatgaaaat
     1981 gtagagaccc acggcgaagc cggccagcgc ggcgatgata tcgtaccagg ggacgacatt
     2041 gcggccggtg ctcggtttca ccggaatcaa gatataggtt gcggataatc cgaggccgag
     2101 gaatagcccc atgtactgct cgttgaaaac caaccagcca agatattgcg gcacgttgag
     2161 tagaaaaaat ataccggtca gggggatggc cactagcaac agccgaccca gcccgccgac
     2221 aaagccgctg agctcccgcc cgcgtacgct ttcttcgatc tccactaggc tctccttgtc
     2281 aacgtggcac gacaacggtc atggccgtga tgagatgacc gatcgctcca ggacctcccc
     2341 agggcaattt tatcacctat cagccgcaga tctctttcgt ccgtgcttcc tgcttggcgc
     2401 cccacacctt catctccttg taaaactgaa ttgcgccgga atgataaggc acggtgaagt
     2461 tggtcagggc ctgcatgtct ttaggccatt tcttgaacag cggatggatt gtctgcagct
     2521 ccggcagatt atcccaccag gctttcaaca tggtgtagac ggttttatcg ctgacgttga
     2581 tcatactcgc caaatgcagc ggataaccaa tgacgtaagt ttcgcccttg acgccggccg
     2641 ccgcttccga tttgaccacc gatgcaccgt agcggccaag gattttattc gccgccgggg
     2701 tgtacgccat gctcaagaaa cgcaccggct ccatggcgtt ggcttcttca acgacgccga
     2761 tgccgacagc ggtgaacgcc gcatcgacct tgccttcggg cacggcgcgc acgccgtcgt
     2821 tcaggttcga cactcgcact tgctgcacgt cggcaggttt caatccgctc gcttccatca
     2881 ccgccaactg ccaggtttgg ttgatcgcat ggccgccgta gtcccaagcc atccgcttgc
     2941 ccttgagatc ggccatctgt ttgatgtccg atttgtcctt gacgatgagt cctgcggtaa
     3001 atggaaacac gccgccggcg acgatgcgca cgcccgggta agctttcttg tagttgccgg
     3061 tcccggtcgc cgccatgttg gaatcgagaa tattgatgat accgaactcg atctcgccgc
     3121 tctccaataa cggcatccag gcgttgggac cattgtaagg ctgcaccttc gccgacacct
     3181 gagtcgtttt gctggcgatc gccgccaggc ccgacgcgat cgcatgagcg ccggtgccag
     3241 ccggatttgt accgatggca atggtccggg gcagctgaat actcttgttg gccgtcggtc
     3301 cgcagtcgag cttggcgtcg gcgatctgca ccggcgccgc gaccagtaga agcgctaaca
     3361 ttagcgcgaa tcccttctta accattaccc cgttactcca ggacccttta ctccgatact
     3421 ccatttctat ttacccgcag atctctttcg tgcgcgcatc atgcttcgcg ccccacacgc
     3481 tggcttcttt gtaaaacttc accgcgccat catgataggg cacggtgaag ttggtgatcg
     3541 cctggacatc tttcgtccat ttcttgaact gcgggtgaat cgtctgcagc tccgccaagt
     3601 tatcccacca ggctttgagc agcgtcgcga cggttttgtc gctgactttg gttgagctcg
     3661 ccaggtgcag cgggtatccg attacccgcg tatcgctgcg aatgcccgcg gtcggatctt
     3721 gtttgacaat aatggcgcca tacttggcga gtattttgtt ggacccggca ttgtcaggca
     3781 ggttgagaaa ccggatcggc tccatggcat tggcctcttc attgatgcca atgcctatgg
     3841 cggcgaaggt ggcgtctact ttgccctcag gtacggctcg aatggcatcg ttcaggttgg
     3901 aaaagcgcac ctgctgcaca tcgctcgcct tgagtcccaa gacttccatg gccgcctgtt
     3961 gccaggtttg ggtgattgca tgaccaccaa agtcccaggc catgcgtttg cccttcaaat
     4021 cagcggcgct tttgatctcc gacttatcac gcaccatgat gctgccggta aacggaaaca
     4081 ctccgccgga gacgatgcgg atcgtcggat aggctttttt gtaattaccg ctcccagtgg
     4141 cggccatgtt agcgtcaaga atgttgatga tgccaaactc gatctcgccc tgttccaata
     4201 atggcatcca ggcgttggga ccgttgtaag gctgcacctt ggccgacatg gttgttacct
     4261 tgctcgccac cgcggacagc ccggaggcca gcgcatgggc gcctgtaccc gccaaattgg
     4321 acgcgatcgc cacagtgcgt ggtaaagcgt tgctcttttc caccgtcggg ctgcaatcaa
     4381 tttttgcctc agcgctgccg cggaaattgc ctaagggcga aagcataaat gccatcacca
     4441 ccgagattac tcggaaattt ttcactctta gcatcagacc atcactcctt tactccatca
     4501 cgctactctt ctcatccgca aatctcccta gtccgcgcgt catgtttggc gccccacatg
     4561 ccggcctcct tgtaaaactt caccgcaccc ggatggtagg gaatcgtgaa attagtgatc
     4621 gcttgaacat ctttagtgaa ctgcttgaac aggggatgga tcgtctgcag ctcggcgtgg
     4681 ttatcccacc acgctttaag catcgcggtg acggtacgct cgttgacttt ggtcgacgtc
     4741 aacaagtgca gcggataacc gataatgttg gtatcaccgc gaacaccagt ggccgcttct
     4801 gctttgacca ccgacgcgcc aagtttggca agtatcttgc cgttcgcctc gctatgagga
     4861 atgctgagaa accgcaccgg ttccatggca ttagcctctt cattgatacc ggtgccaagc
     4921 gatgcgtagg tcccatccac ttttccctcg gcaacaccgc ggatgccgtc gttagaagtc
     4981 gttaaccgca cctgagtgat atcggttggt tttatcccgc cggtttccaa agcggcgtta
     5041 agcaacgcct gtatcacggc atggccgcca aaatcccacg ccagccgttt acccttgagg
     5101 tcgctgatct gtttgatgtc tgatttatcg cgcaccatga tgccgccggt aaatggaaat
     5161 actccgccgg taagcacgcg cagactcgga taggctttct tgtacgtccc agtgccggtc
     5221 atcgccatgt gcgcatcgag aatattgatg atgccaaact ccagctcgcc gctttcgagc
     5281 agtgggacaa acgtattcgg cccggcgtaa ggctgcactt tggcgatgat cggtgtcgcc
     5341 ttgctcgcca ccgcggccaa tccagagcct aaagaaaacg cgcccgtacc tgccgggctc
     5401 gtgccgatgg cgacgctgcg agcgagttcg ttacccttgt tgatcaccgg cccacagtcg
     5461 accttggcgt tggcactgcc gacaacaaac gtttgagcaa cgagcagagt gacgacagtc
     5521 gccgcaccaa tcaattgctt gctgtgc
//
LOCUS       DASQKS010000389         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1877070, whole genome shotgun sequence.
ACCESSION   DASQKS010000389 DASQKS010000000
VERSION     DASQKS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877070"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..597
                     /locus_tag="VE170_10105"
     CDS             181..597
                     /locus_tag="VE170_10105"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ15833.1"
                     /translation="MIEILDQMKEMIDPALANGCPCILATVSGDGEPDIGYKGSMMVF
                     DKQSLAYLERTKRVHLKNLKENPKVIVLFRDAKTKVAWRFHGTATLHESGPTWEQGMA
                     RTVKEELDKDPERKGIIVQIQLDKITNMGGQVLQSR"
     gene            complement(684..1745)
                     /locus_tag="VE170_10110"
     CDS             complement(684..1745)
                     /locus_tag="VE170_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15834.1"
                     /translation="MSEAEILRELDEKLRDLSVEGLWAPASAADLATYSKDPHTTVLP
                     HVWKWPALYDAIQTVANMHGLDGKAERRVLRLINPAYLQQHNRRQRTTTHTMLMTLQL
                     LKPGEIAHDHRHNFAAFRFILKGRGAYTVVEGEEIPMAEGDLVLTPSMTWHGHRNNGE
                     PVVWLDGLDNPLLFLLQAITWEAYPGGLQPIKERSEHTAPRTGTTRPVWEKISDRPAY
                     NLHYKWQDTYEKLQQLSQGPCSPFDGVALEYVDPEGGHTMPTMSCGMQMLRPGEATKT
                     HRHNYSVVYHAFRGRGATVIDGQRFEWQQGDCFVVPLWSWHSHQNGSNSDEALLFSVS
                     DLPVMAALKLLREEVGEGA"
     gene            complement(2066..3034)
                     /locus_tag="VE170_10115"
     CDS             complement(2066..3034)
                     /locus_tag="VE170_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15835.1"
                     /translation="MKLKAALIILSLLTLSVPMQRGEAADKARIAVTNFNLSFLPVGV
                     AIKRGFFKDEGLEVEVIRMNTPTTLTAMMTGDVGYTLLFGSVVRAALRGLPIRALASL
                     LDSPTYALIARAEYKTLKDLKGKTVGIANFGGTDEVLSKLIFKGQGIDPERELKFIAL
                     GTDRARLAALKEGLVDVAIISPPGDTMGRQMGFNVLTRAHEHFNFPFLGVGTNLKSLK
                     ERPLEVRRVTKALVRANRFIRDDKEGAVRVLVDWAKIEREHALASYDSAWKVFSPDGN
                     ISPEGLRLVLEQAKIELKLTREIPLSEIVDPLPLQEAQRELAIRRP"
BASE COUNT          673 a          913 c          856 g          686 t
ORIGIN      
        1 atactagtac tcccaaacct tcgcgtcttc ataaacttcg gggcgagaac cttagttctg
       61 ctgcttgaac gctaaaatcc tgccgcatgc caggttcgtc aattacgtag tggcgcaccc
      121 gggaatcggt ttgacgaggg accaatagtt taataggttg agttcttcag gaggtgaccc
      181 atgattgaaa ttctcgacca gatgaaagaa atgatcgacc cggctttggc caacggttgt
      241 ccgtgcatcc tcgccacggt ttccggcgac ggcgaaccgg acatcggcta caagggcagc
      301 atgatggtgt tcgacaagca gtcgctggct tatttagagc gcaccaagcg ggtccatcta
      361 aagaatctca aagaaaaccc caaagtcatc gtgctgttcc gcgatgccaa aaccaaagta
      421 gcctggcgct ttcacggcac cgcgaccctg cacgaatcgg gaccgacctg ggaacagggc
      481 atggcgcgca ccgtcaaaga agagttggac aaagatcccg agcgcaaggg catcatcgtg
      541 caaatccagc tcgataagat caccaacatg ggcggccagg tgttacaaag ccgctgatcc
      601 ggatgatcga gcaaccggat tcgtggtttg caatctgtaa tgtgaaattc tcaacttgag
      661 atggtgcccg gcaacgcgcg atctcacgcc ccctcgccaa cctcttcacg cagcagttta
      721 agcgccgcca tcaccggcaa gtcgctgacc gaaaagagca gcgcttcgtc gctgttcgag
      781 ccattctggt ggctgtgcca gctccacaac ggcacgacaa aacaatcgcc ctgctgccat
      841 tcaaaccgtt gtccgtcgat caccgtcgcg ccgcggccgc gaaacgcgtg atagacgacg
      901 ctgtaattgt gccgatgggt cttggtcgct tcgccgggac gaagcatttg catgccacag
      961 gacatggtcg gcatggtgtg gccaccctcg gggtcgacgt attcgagggc aacgccatcg
     1021 aacggactgc atggcccctg gctcagctgt tgcaatttct cgtaggtgtc ctgccatttg
     1081 tagtgcaagt tataagccgg acgatcggaa attttttccc ataccggccg ggtcgtcccg
     1141 gtacgcggcg cagtatgttc ggagcgctct ttgatcggtt ggagcccgcc aggatatgct
     1201 tcccaggtga tcgcctgaag caagaacagc agtgggttat cgaggccgtc taaccacacc
     1261 accggttcgc cgttgttgcg gtggccgtgc caggtcatcg acggcgtaag caccaggtcg
     1321 ccttcggcca tgggaatttc ttcgccttcg acgacggtat aggcgccgcg gcctttgagg
     1381 atgaagcgga aggcggcgaa attatgccgg tgatcgtggg cgatctcgcc gggcttgagc
     1441 agctgcaacg tcatcagcat ggtatgggtg gtggtgcgtt ggcggcgatt gtgttgctgc
     1501 aaataagccg gattgatcaa ccgcagcacg cgccgttcgg ccttgccgtc gagcccatgc
     1561 atattggcca cggtttgaat ggcgtcgtac agggccggcc atttccacac atggggcagc
     1621 acggtggtgt ggggatcttt actgtaggtc gccaggtcgg cagccgacgc cggcgcccac
     1681 aacccttcga cgctcaagtc ccgcagcttc tcatcaagtt cacgcaatat ctctgcttcc
     1741 gacattttgt gctccttagc gtcatgtgtc cgcgcagcga ttattcgcca cgaagtgaat
     1801 catgatctac gctcgtctac agaatatgaa aaggcatggg tttcgctcta taggcagtct
     1861 actcgacgcg tcattcccgc gcccgcggga atccaggttt gatctgcgcc caattagcct
     1921 ggatacccgc tttcgcgggt atggacggaa cccagatacc cctcttcgtt ttggcgacca
     1981 ttccttacat gtattttcgc aggaggagaa gaggattacg aagtcaaaat ccgcggaccc
     2041 atcgctgctt cgcgcccctc gcccgttacg gccttctgat cgccaattcc cgctgcgcct
     2101 cttgcaacgg cagcggatcg acaatttcac tcagcgggat ttcgcgggtc agcttgagct
     2161 cgatcttggc ttgctccaac accagtcgta aaccttcggg cgaaatattt ccgtccgggc
     2221 tgaaaacttt ccaagctgag tcataagacg ccaacgcatg ctcgcgttcg atcttggccc
     2281 aatctaccaa cacgcgcacc gcgccttctt tgtcatcgcg gatgaaacga ttggcgcgca
     2341 ccaatgcctt ggtcacgcgg cgaacttcca gcggccgctc tttcaatgat ttaagattag
     2401 ttccgacgcc aagaaacgga aagttgaaat gctcatgagc ccgagtcaac acgttaaagc
     2461 ccatctgccg gcccatggta tcgcccggcg gcgagatgat cgcgacgtcc actagccctt
     2521 ctttgagtgc ggctagacgg gcccggtcgg tgcccagggc gatgaatttg agctcccgtt
     2581 ccgggtcgat cccctggcct ttgaaaatca gtttcgacag cacttcgtcg gtgccgccga
     2641 agttggcgat gccgacggtc ttgcctttca aatccttgag cgtcttgtat tctgcccggg
     2701 caatcaacgc ataggtcgga ctgtcgagca ggctcgccag agcgcggatc ggcagaccgc
     2761 gcaaggccgc gcggaccacc gaaccgaaca gcagcgtgta accgacatcg ccggtcatca
     2821 ttgccgttaa agtggtcggc gtattcatgc gaatcacttc cacctcaagc ccctcatcct
     2881 tgaagaaacc tcgtttgatc gccaccccga ccggcaaaaa ggacaggttg aaattagtca
     2941 ccgcgatgcg cgccttatcc gcggcttcgc cgcgctgcat tggcacactg agcgtgagca
     3001 aactcaagat tattaaagcc gccttcaatt tcacaattat tccccaagtc attagaattt
     3061 taggggcgag agattgtcgg ttccgagaat tggttcacca cgaagtgaat catgatcgac
     3121 gctcgtct
//
LOCUS       DASQKS010000390         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_630412, whole genome shotgun sequence.
ACCESSION   DASQKS010000390 DASQKS010000000
VERSION     DASQKS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630412"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VE170_10120"
     CDS             complement(<1..1373)
                     /locus_tag="VE170_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ15836.1"
                     /translation="MADSPIKKSEKRWQSDVIVDMIKKYDFEYIALNPGASYRGLHDS
                     LVNYGENDPPMLTCQHEKIAVQIAHGYARAKGKPMIAIVHNLVGLLHAPMGIYYAYLD
                     RAPVFIMGATGPLAEPKRRPFIDWIHTANVQGEAIRNFVKWDYQPSTVDGVPGAFARA
                     YSVMMSARQGPIYMCYDAGLQEAELDHDVEMPPAGSAEVPTAPSPDPVALAKAADTLA
                     AANRVAIIADFAARPPHGWKHVVDIAETLGASVWDVGSRLNFPSDHPLNLTADFDGCY
                     DGVDVVLTLDIADYEKPTHVRDIATRTVVSKVQANATWIDIGFTDIEISKWSMDYARP
                     YYAQQRMTADPVTATPQLTQLLKERIAKTPGLAEKIAKRTEAIGKRHAETRAKWAKQA
                     QEHWDQKPMTVPRLASEVWEAIKGEDWVLTAGDLGSWGKKLWNFDRPYCHGGRELGTG
                     TQIGLSLG"
     gene            complement(1546..3315)
                     /locus_tag="VE170_10125"
     CDS             complement(1546..3315)
                     /locus_tag="VE170_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ15837.1"
                     /translation="MAETSIKKSGERWQSDIIVDLIKQFDFPYIALNPGASYRGLHDS
                     LVNYGENKPEMLLCNHEKIAVQIAHGYAKATGKPMIAIVHNVVGLLHAPMGIYYAYID
                     RSPVFIIGATGPMDEKLRRPFIDWIHTAFAQGDVVRGFTKWDYQPGSIHGVPDSFARA
                     YSIMMSEPQGPVYMVYDSAMQEAKLTEDVPVPNFHIKVPSRVAPEQAVIDQAADMLVA
                     ANNPLLLTEYAARMPMGFQSTVELAETVGASVYDINKRLGFPNKHPLSVSFHNDAFNG
                     VDLVTALDVVDWTRGSHKLNTQTREIQVMTAADCKWIDVGFADIEISKWATDYNKHHN
                     WDHRVLGDSALSIPALTAACKKRIDGDKTLTGRIAERKKTIGERHDRQWQKWQDQVKE
                     QWNERPMTEARLAHEAWQVIKGEDWVLTANTLKEWVHKIWDFDKPYRHLGRELGTGTQ
                     IGMSIGAALAYKGTGKLVVDVQPDGDLMFDLGALWMPVKYNIPMLILMYNNRAYYNDW
                     AHQIHMAHQRGTDPQRAYIGMDLEGPPPDFAHIAKGIGWYSEGVIEDPAEVGPALKRA
                     IAQVKAGKPALVDAVVWRRGENA"
     gene            complement(3491..4195)
                     /locus_tag="VE170_10130"
     CDS             complement(3491..4195)
                     /locus_tag="VE170_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15838.1"
                     /translation="MLEVTNLSLSYGGAAALNGVSLAIAKGEMVALIGANGAGKSSFI
                     RAIAGIERPITGQIVFAGRDIAALESHQICNLGLGQVAEGRQLFPTLTVLENLQLGAM
                     LQRARGQTTQSLNRVFALFPRLDERRKQLAGTLSGGEQQMLAIGRCLMGCPELIMFDE
                     PSLGLAPNLVQETLLAIRQLNHSGLTVLLVEQNVAASLKICRRGYVLENGSIVMTGNG
                     NELLNDDGVRQAYLGL"
     gene            complement(4201..>4539)
                     /locus_tag="VE170_10135"
     CDS             complement(4201..>4539)
                     /locus_tag="VE170_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15839.1"
                     /translation="RDLPAAALTLPDRKRLEVARALATQPKLLLLDEAMAGLRPTECD
                     QMVAVLRELNRREGITILLIEHVMRAVMALADNVIVLHHGEIIGAGLPGEVVRQPAVV
                     ASYLGENSGL"
BASE COUNT          852 a         1267 c         1397 g         1023 t
ORIGIN      
        1 ccgagcgata agccgatctg ggtaccggtt ccaagctccc gcccaccatg gcagtacggc
       61 cggtcgaagt tccatagctt cttgccccat gacccaagat cgccggcggt gaggacccaa
      121 tcctcccctt tgatcgcttc ccagacttcg ctggcgagac gcggcacggt catcggtttt
      181 tgatcccagt gttcctgggc ttgcttggcc catttggcgc gggtttcagc atggcgtttg
      241 ccgattgctt cggtgcgctt ggcaattttt tcggcgagcc ccggggtttt ggcaatacgt
      301 tccttcaaga gttgagtcaa ctgcggggtt gccgtgaccg gatcggcggt catgcgttgc
      361 tgggcgtaat agggccgggc gtaatccatc gaccatttgc tgatttctat gtcggtgaag
      421 ccgatatcga tccaggttgc attcgcctga accttgctga ccaccgtgcg cgtcgcgatg
      481 tcgcgtacat gggtcggttt ttcgtagtcg gcgatgtcga gcgtgagcac gacgtcgacg
      541 ccgtcgtagc aaccatcgaa gtcggcggtg agattcaacg gatgatcgct cgggaaattc
      601 agccgcgagc cgacgtccca caccgacgcg cccagggttt cggcgatgtc gacaacatgc
      661 ttccagccat ggggtgggcg ggcggcgaag tcggcgatga tcgcgacccg attggccgcg
      721 gcgagagtat cggcggcttt ggccagcgcg acagggtccg gcgaaggcgc ggtgggcact
      781 tcggcgctgc cggcgggcgg catttcgaca tcatgatcga gctcggcttc ctgtaaaccg
      841 gcgtcatagc acatatagat cggaccctgg cgcgcgctca tcatcactga gtaggcgcgc
      901 gcgaaggcgc ccggcacacc gtcgacggtg gacggctgat aatcccattt gacaaaattg
      961 cgaatcgcct cgccctgaac gttggcggtg tgaatccagt cgataaacgg ccggcgcttg
     1021 ggttcggcga ggggtccggt ggcgcccatg atgaacaccg gcgcgcggtc gagataagcg
     1081 taatagatgc ccatcggcgc gtgtagcagg ccgaccaagt tatgcacgat ggcgatcatg
     1141 ggcttgccct tggcgcgggc gtaaccatgg gcgatctgca cggcgatctt ttcatgctgg
     1201 caggtgagca tcggcggatc gttctcgcca tagttgacca gcgagtcatg caagccgcgg
     1261 tagctggcac cgggattgag cgcgatgtac tcgaaatcat atttcttgat catgtcgacg
     1321 atcacatcgg actgccaacg tttttcggat tttttgattg gtgaatctgc catgcggata
     1381 tcctccttaa gaataaattc attaaccaca aagggcacaa agatcacaaa gcaggccggt
     1441 tttaaaccgg cctacaaatg ttttgcgctc ctcgtggcta aatttccgag tccgaatatt
     1501 ttttctgtgt gaccctttgt gctcttggtg gtgaaatcgt ttcttctagg cattctcgcc
     1561 gcggcgccag acgacggcat cgaccagagc cggtttcccg gctttgactt gggcgatggc
     1621 gcgtttcagg gccgggccga cctcggcggg atcttcgatg accccttcag agtaccagcc
     1681 gatgcccttg gcgatatggg cgaagtcggg cggcggccct tcgaggtcca tgccgatgta
     1741 ggcgcgctgg ggatcggtgc cgcgttgatg cgccatgtgg atttgatgtg cccagtcgtt
     1801 gtagtaggcg cggttgttgt acatcaagat cagcatcgga atgttgtact tgaccggcat
     1861 ccacagggcg cctaagtcga acatcagatc gccgtcgggc tgaacgtcga cgactagctt
     1921 tcccgtgcct ttataagcca gggcggcgcc gatcgacatg ccgatctgcg tgccggtgcc
     1981 caactcgcga ccgaggtggc gatagggctt gtcgaaatcc catattttgt gcacccattc
     2041 tttcaaagta ttggcggtga gcacccagtc ttcgcctttt atcacctgcc aggcttcgtg
     2101 cgccaggcgc gcttcggtca tcggccgctc gttccactgc tccttcactt gatcctgcca
     2161 cttttgccat tggcgatcgt ggcgttcgcc gatggttttc ttgcgctcgg cgatgcggcc
     2221 agtgagggtt ttgtcgccgt cgatacgttt cttgcaggcc gcggtgagcg ccggaattga
     2281 cagcgcgctg tcgcccagca cgcgatgatc ccagttgtgg tgtttgttgt agtcggtggc
     2341 ccacttgctg atttcgatgt cggcgaaacc gacatcgatc cacttgcagt ccgccgcggt
     2401 catgacctgg atttcgcggg tctgggtgtt caatttgtgc gagccgcggg tccagtcgac
     2461 gacatcgaga gcggtgacca gatcgacacc attgaaagcg tcgttgtgga agctaacgct
     2521 caacggatgt ttattgggga atcccaagcg cttgttgatg tcgtagaccg aggcgccgac
     2581 ggtttcggcg agttcgacgg tggattgaaa acccatgggc atgcgcgccg cgtactcggt
     2641 caaaagcagc gggttattcg ccgccaccag catgtcggcc gcttggtcga tgaccgcctg
     2701 ttcgggggcg acgcgggacg gtactttgat atgaaagttg ggcaccggca catcctcggt
     2761 cagcttggct tcctgcatcg ccgaatcgta gaccatatag accggccctt ggggctcgct
     2821 catcatgatc gagtaggcgc gcgcgaagga atccggcacg ccgtgaatgc tgccgggctg
     2881 gtaatcccat ttggtgaaac cgcgcacgac atcgccttgg gcaaaggcgg tatgaatcca
     2941 atcgataaac ggccggcgca atttttcgtc catcgggccg gtggcaccga tgataaatac
     3001 cggcgaccgg tcaatgtagg cgtagtagat gcccatgggc gcatgcaata gaccgacgac
     3061 attgtgaacg atggcgatca tcggcttacc cgtggccttg gcgtagccat gggcgatctg
     3121 cacggcgatc ttttcatggt tgcacagcag catctccggc ttgttctcgc cgtaattgac
     3181 caaggagtca tggagaccgc ggtagctggc gccgggattg agagcgatat aggggaaatc
     3241 gaattgtttg attagatcga caatgatgtc ggactgccaa cgttcgccgg attttttgat
     3301 gctggtttct gccatggcgt tatcctcctg aaaatacccc gtgccggtta tttttcgatc
     3361 tcctttgtta tctgggaaat ccctttgtgg caagggctat ggggcacgaa gtgcgctctg
     3421 ttcaaggttc aagggttcaa aggttcaagg tcagagggat accactgtaa tttcagcgga
     3481 gtcaggagtt ctataacccg agatacgcct ggcgtactcc atcgtcgttc agcagttcat
     3541 tgccattgcc ggtcatgacg atgctgccgt tctccagcac atagccgcgt cggcaaatct
     3601 tcaacgacgc ggcgacgttc tgttcgacca gaagcaccgt caggccgctg tgattcaact
     3661 ggcgaatggc gagaagcgtt tcctgcacga ggttgggcgc caggccgagg gatggctcgt
     3721 cgaacatgat gagttccggg cagcccatca ggcaacggcc gatggcgagc atctgttgtt
     3781 cgccgcccga taaagtgccg gcaagttgct tccgtctttc atccagacgc ggaaagagcg
     3841 cgaaaactct gttgagcgat tgtgtcgttt gcccgcgtgc gcgttgcaac atcgcgccta
     3901 gttgcaaatt ctccagcacg gtcaaagttg gaaacagctg gcgcccctcg gcgacttggc
     3961 cgaggccaag attgcatatc tggtgcgact cgagcgcggc gatgtcgcgg ccggcaaaaa
     4021 caatctggcc ggtgatcggc cgctcgatac ccgcgatggc gcgaatgaac gaagatttgc
     4081 cggcgccgtt ggcgccgatc agcgcgacca tctcgccttt ggcgattgcc agcgagacgc
     4141 cgttaagcgc tgcggcgccg ccgtaactta gggagaggtt ggtaacctca agcaaagcgc
     4201 tcataaaccg ctattttcac ctaagtagct tgccaccacc gcgggttggc gcaccacttc
     4261 gccgggtagg cctgcgccga tgatttcgcc gtgatgcagc acgatcacgt tgtcggccaa
     4321 cgccatcacc gcgcgcatga catgctcgat caagagaatg gtaataccct cgcgccggtt
     4381 gagctcgcgc agcacggcga ccatctgatc gcattcggtg ggacgcaggc cggccatcgc
     4441 ttcgtcaagc agcaagagtt tcggctgggt cgctaatgcg cgcgcgacct cgaggcgttt
     4501 tcggtccggc agtgtgagcg ctgcagcggg cagatcgcg
//
LOCUS       DASQKS010000391         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_197468, whole genome shotgun sequence.
ACCESSION   DASQKS010000391 DASQKS010000000
VERSION     DASQKS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197468"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VE170_10140"
     CDS             complement(<1..427)
                     /locus_tag="VE170_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYJ15840.1"
                     /translation="MTEKRKSPSEIMQENIRLRAQVAQLEDRLDHLSEHGAAAKSLQY
                     AEALHADVMNAVSDVVLVTDIAGRLTYASPNSKLIFGQSPAEMLKVGRIGLLLPTNLY
                     DPDALEQRGEISNLECQIRDPIGRARDLSITVRRLGKESA"
     gene            complement(539..1210)
                     /locus_tag="VE170_10145"
     CDS             complement(539..1210)
                     /locus_tag="VE170_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HYJ15841.1"
                     /translation="MLDSIVLSFFHCFSFTSNLVPMAPTIRIAEPADAAALLAIYAPY
                     CESSPISFEVVAPSVEQMAQRIADIAADYPWLVCEIDGVIAGYVYASRHRERAAYRWI
                     VEVTVYISERYRRLGVGRALYTSLFEILAEQKYVKAFAGVTLPNPQSVGLHESLGFTL
                     IGVYPGVGYKLGRWLDVGWWQLQLRSEAIPPPEPVPFAALRSTAVVVNALSAGERLLT
                     HVAGA"
     gene            complement(1288..1755)
                     /locus_tag="VE170_10150"
     CDS             complement(1288..1755)
                     /locus_tag="VE170_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HYJ15842.1"
                     /translation="MEYEIKLEQSVGRPLAIVRRRASRQELSKIVPDACGVVWTVIRS
                     QQVPGAGRHVAVYLDDKINLEVGVELEAPFDGYGEVIASKLPAGPVATTTHYGPYGQL
                     HQAHDAIHQWCQSNGRTLAGPSWDIYGHWKDEWNTDPSKITTDVCYLVHANGQ"
     gene            complement(1822..2043)
                     /locus_tag="VE170_10155"
     CDS             complement(1822..2043)
                     /locus_tag="VE170_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15843.1"
                     /translation="MTSEQFKSTLRLQPFPQFTIRMADGRTFDVSHPDFVAQSPSGRT
                     VIVFQPDESYSVLDLLLMTELQVANGQHH"
BASE COUNT          469 a          618 c          674 g          446 t
ORIGIN      
        1 aggcgctctc tttgccgagg cggcggacgg tgatcgaaag atcgcgggcg cggccgatcg
       61 ggtcgcgaat ttggcattcc aagttggaaa tttcaccgcg ctgttcgagc gcgtcggggt
      121 cgtagagatt ggtgggcagc agcaggccga tgcggccgac cttcaacatt tcggcgggcg
      181 actggccgaa gatgagtttc gaattcggtg aggcgtaggt cagccggccc gcgatgtccg
      241 tgactaatac gacgtcagag accgcattca tcacatcggc atgaagagcc tccgcatact
      301 gaagcgactt ggccgcggcg ccgtgctcgc tcaagtggtc gagccggtct tccaattgcg
      361 cgacctgagc gcgcaggcga atgttttctt gcatgatctc agacggtgat ttacgttttt
      421 ctgtcataac cgtgtacgcg accttgatcg actgccgcgc ggcggggcgg agcttttgtc
      481 gccaccgatt tgcgggaagg cgcggcgatg tattataacc gaaagcggcc cggttgcatc
      541 aagccccagc tacgtgcgtc aaaagccgct cgcccgctga caacgcattc acgacaacgg
      601 ccgtcgatcg aagggccgca aagggcactg gctccggtgg tggaattgcc tccgaccgca
      661 gttgaagctg ccaccagccg acatccagcc aacgacctaa tttatagccc acgcctggat
      721 atactcctat gagcgtaaaa ccgagcgatt catgcagacc gacgctttgc ggattgggca
      781 gcgtcacgcc ggcaaatgcc ttgacgtatt tttgttcggc caaaatctcg aacaagcttg
      841 tgtagagcgc ccgccccacg ccgaggcggc gatagcgctc ggaaatgtag acggtcactt
      901 ccacgatcca tcgataagcg gcccgctcgc gatgccgcga cgcgtagacg tacccagcga
      961 tgaccccatc gatttcgcat accagccatg gatagtcggc cgcgatgtcg gcgatgcgct
     1021 gcgccatttg ctcgaccgag ggggcgacca cttcgaacga gatgggcgag gattcgcagt
     1081 agggcgcgta aatggccaag agggcggcgg cgtcggcggg ctcggcgatg cggatggtgg
     1141 gcgccatggg aacgaggttg gaagtgaaac taaagcaatg aaaaaacgaa agaacgatac
     1201 tatccagcat cttcgagcag agagtcgaag tcaacgtcga aagatggcgc gagagggcgc
     1261 acttgtgctt acgcctggcg actgatgtta ctggccgttt gcatggacca gataacaaac
     1321 atccgtcgtg attttcgagg gatccgtgtt ccactcatct ttccagtggc cgtaaatatc
     1381 ccagctcggg ccggccagtg tgcggccgtt gctttggcac cactggtgaa tcgcgtcgtg
     1441 cgcttgatgg agttggccgt aagggccgta gtgagttgtt gtggcgaccg gcccggccgg
     1501 cagctttgag gcgatcactt ccccataacc gtcgaacggc gcttcgagtt cgacgcccac
     1561 ctcgagatta attttgtcat ccagatagac cgcgacgtgc cggccggcgc caggaacttg
     1621 ctgcgagcgg atcaccgtcc acacaactcc gcaggcgtcg ggaacgatct tcgagagctc
     1681 ttgccggctc gcccgccgac gcacgatggc aaggggccgg ccaacggatt gctcaagttt
     1741 gatttcgtat tccatgcgcg ggtgttccgc tcgctattga tcgcgctccg cgcctgccgg
     1801 ccgataagat cagcaggccg attagtgatg ttgcccgttg gcgacttgca attccgtcat
     1861 cagcaaaaga tcgagcacgc tgtagctttc atcaggctgg aaaacgatta ccgttcggcc
     1921 cgatggcgat tgcgcgacaa agtcggggtg cgagacatca aatgttcgcc catccgccat
     1981 gcgaatggtg aactgcggaa aaggctgaag gcgcaacgtc gatttaaact gttcggaggt
     2041 catatgccaa ttatatctcc tagcgaatca accgctatcg agagaacctg gccgttgtct
     2101 cggtcattat gcccgcaata cttgcacaaa aaaagccggc ccttttgggg ccggctatga
     2161 aaaatggcgg ggacaggatt cgaacctgcg acctcgaggt tatgagt
//
LOCUS       DASQKS010000392         6176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2736563, whole genome shotgun sequence.
ACCESSION   DASQKS010000392 DASQKS010000000
VERSION     DASQKS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736563"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /gene="cysS"
                     /locus_tag="VE170_10160"
     CDS             <1..217
                     /gene="cysS"
                     /locus_tag="VE170_10160"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522125.1"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYJ15844.1"
                     /translation="GLLQEGYFERKKQRWLKNSILSEGEILAAIGLRNEARKAKNFPE
                     ADRIRDELNQRGVAIEDTPEGTKWKVR"
     gene            202..2016
                     /locus_tag="VE170_10165"
     CDS             202..2016
                     /locus_tag="VE170_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15845.1"
                     /translation="MESKMMGGAGGGFLIAVGLSAAVAGNWSVLAQETAGGGQTEINL
                     RRSAQSRPYQGREVEGEERQIAPGDSLWRILVRDKNLPGQHFHSYLVVIQGLNPQIKD
                     PQILRVGDKIFIPLRPDQVASARPTSNTPADSSNSSRGTTINYRVKAGEHLYQILREQ
                     LKPTDDRKLAQYYALAKDLNPERKNWDTLVEGDIIRLPTLGQGNQVAAAPALKPLPSI
                     ETTPTESKRTLESRPTDGTKPAASAVDRSQAMRAPARENMALFARVAEALGGEVQNSG
                     EEVVSIKDGNLRFDKGSYPVVYSAALRQRVVIDPDGKIPSTVRSRLADPSVGTAILTM
                     ANGVSIQGAVSQLLAGLGYQTLPPDRPVIVQEEGVAYEAKGGWMALAAAQSDKVQEIY
                     VISLTDNAAEIPDYLKSHLQKSGLHLKDILLPGAPNQPLAPAKAESEGVFTQIKTWPR
                     DKREMVDALLFSFGVPFGVAESISVQLRDGLHLDARSDRVFEVSGTKTVLFFQRPDPE
                     VVKALQEIPGLRAVDLDLTNLSSREVINRMVNLLGDQTTYREHRFAAGSGGAKDRLTV
                     TVSGFHLARRSMFITDRQIPAGLHRFFFEKGLDIVYFQ"
     gene            2030..2106
                     /locus_tag="VE170_10170"
     tRNA            2030..2106
                     /locus_tag="VE170_10170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2064..2066,aa:Arg,seq:tcg)
     gene            2750..3169
                     /locus_tag="VE170_10175"
     CDS             2750..3169
                     /locus_tag="VE170_10175"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HYJ15846.1"
                     /translation="MKHRLRKQIEVVAGLILAEQKLLVCQRHRSGAFPYKWEFPGGKI
                     ESEESAEAALARELKEELDISTGEFSFIYRCNHAYANGLDVSLSFYRVINYQGVIKNL
                     VFEQIAWSNLEELTKFDFLEGDVSLVHQLANGELSVN"
     gene            3278..3886
                     /locus_tag="VE170_10180"
     CDS             3278..3886
                     /locus_tag="VE170_10180"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYJ15847.1"
                     /translation="MKRIIITLAAVCLLVTAAPPARADVYMFKDKQGILTFTNVPNHV
                     GYKRILRDSGVTLGGGNVTGTAFDELISSASGRYNVDPDLIRAVIKTESDFNTNARSH
                     KGAMGLMQLMPETARMHNVSDAYNPNENVDGGVRHLRMLLERYQGNFELSLAAYNAGA
                     GAVEKHGGIPPFNETREYVRKVLRFYDAFRDTGLNSLQQARQ"
     gene            3924..4493
                     /gene="pgsA"
                     /locus_tag="VE170_10185"
     CDS             3924..4493
                     /gene="pgsA"
                     /locus_tag="VE170_10185"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HYJ15848.1"
                     /translation="MVWTLPNLLSLFRILIIPPLVYLLSFPGRSTSLWAAVLFLVASI
                     TDYFDGYFARRNKSVSDLGKILDPLADKLMVASALIMLAAMDRPGEPSVPAWLVVVIL
                     ARESAVTVIRGIALSDGIVMQAETLGKYKFIMQAYAIVGLLVHYRYWGFDFFVAGMYF
                     LVLSAVIAAWSGVNYHVQFFRLRLVRSAR"
     gene            4547..4623
                     /locus_tag="VE170_10190"
     tRNA            4547..4623
                     /locus_tag="VE170_10190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4581..4583,aa:Gly,seq:gcc)
     gene            complement(5024..5800)
                     /locus_tag="VE170_10195"
     CDS             complement(5024..5800)
                     /locus_tag="VE170_10195"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYJ15849.1"
                     /translation="MQVFFEKRLLEYLENQDLPYIIVARLTAVVRKLVVHQIPATAWR
                     AVARGIDVADLEVSLPNWKTVKRRFVCLRQEISERPQASGRRLIDYPGYSYRVMVTSM
                     PYAAEIVTRMYAGRADSENRIKELKEDLSLDTFCLKSFDATDAAFRMGGVLYNLLADF
                     RETVLPRSWFEKRLRAVRDFVFLVGADLIHQGRRVRVRFAIPEPDRPELLRCLRTKSE
                     GLPIAAQLEWSLPNQTPTHPVKIISPIPLLSPLSTVSQSP"
     gene            complement(5784..>6176)
                     /locus_tag="VE170_10200"
     CDS             complement(5784..>6176)
                     /locus_tag="VE170_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYJ15850.1"
                     /translation="RFGSPDTLRRLFLRFGYREVTEVSERLMRFSLTRMRPILMGHTL
                     DLDSTILCRYGEQEGSLKGYNPKKPGRPSDHPLVGFLAEKRRLLWATLRSGNSGSANG
                     CVEFLKQSLTVLPDGQRIGLVRADAGFF"
BASE COUNT         1455 a         1646 c         1635 g         1440 t
ORIGIN      
        1 gggattactt caagaaggtt atttcgagcg caagaaacag cgctggttaa aaaacagcat
       61 cctatcggaa ggtgagatcc tggcggcgat cggcctgcgc aacgaagcgc gcaaagccaa
      121 gaattttccg gaagcggatc gcattcgcga tgagctcaat cagcgtggcg tcgcgatcga
      181 agacacgccg gaggggacga aatggaaagt aagatgatgg gcggggctgg tggcggattc
      241 cttattgcgg ttgggttgtc ggcagcggtc gctgggaatt ggtcggtttt ggcgcaggaa
      301 acggccggcg gcggccaaac ggaaatcaat ttacggcgct cggcgcagag ccggccttat
      361 cagggacggg aagttgaagg tgaggagcgc caaatcgctc ccggagatag tctgtggcgc
      421 atattggtgc gcgacaaaaa tttgcccggg cagcactttc atagttattt ggtggttatt
      481 caaggtctga atccgcaaat caaagatccc caaatcctcc gggtcggcga caagattttc
      541 atcccgctgc ggccagatca agtcgccagc gcacgcccga cgtcgaacac accggcagat
      601 agctcgaaca gcagccgggg aaccacgatc aactatcggg tcaaagccgg cgagcatcta
      661 taccaaatcc tgcgcgaaca gctaaaacct accgacgatc gcaaattggc ccagtattat
      721 gctttggcca aagacttgaa tcctgagcgg aagaactggg acaccctagt cgaaggtgac
      781 atcattcgct taccgacact aggccagggg aatcaagtcg cggcagcgcc ggcgctcaaa
      841 cctttgccat cgattgaaac aacacctacg gaaagtaaac ggacgttgga atctcggccg
      901 actgacggga ctaaaccagc agcctccgct gttgatcgct cccaagcgat gcgcgctccg
      961 gcccgcgaga atatggcact ttttgccaga gtcgctgagg cgctcggcgg tgaggtgcag
     1021 aatagtggtg aagaagtagt atcgatcaaa gacggcaatc ttcgtttcga caagggcagc
     1081 tacccggtgg tgtacagtgc ggcattgcgg cagagggtgg tgatcgaccc ggacggaaag
     1141 attccttcga ccgtgcgtag ccggttggcg gatcccagcg tgggcacggc aattctgaca
     1201 atggccaacg gcgtttcgat ccaaggcgcg gtgagtcagt tgcttgctgg gttaggctat
     1261 cagacgttgc cccccgatcg accggtgatc gttcaagaag aaggggttgc ctacgaggcc
     1321 aagggtggct ggatggcgct ggcggcggcg caaagcgaca aggtacagga gatttacgtc
     1381 atcagcctga ccgacaatgc ggcggaaatc cccgactatc tcaaaagcca tctacaaaaa
     1441 agcggtttgc acctgaaaga tattctcttg ccgggcgcgc cgaaccaacc cctggcgcca
     1501 gccaaggccg agtccgaggg tgtctttact cagataaaaa cctggccgcg cgacaagcgc
     1561 gaaatggtcg atgcgctatt attttccttc ggtgtgccgt tcggcgtggc ggaaagcatt
     1621 tccgtgcagc tgcgcgacgg cttgcacctc gacgctcgta gcgatcgcgt ttttgaggtc
     1681 tccggcacaa agaccgtgtt atttttccaa cgtcccgacc ctgaagtcgt aaaggcgcta
     1741 caagaaatcc cagggttgcg cgctgtcgac ctcgatttga ccaacttaag ttcgcgagaa
     1801 gtgataaatc gcatggtgaa cttgttgggc gaccagacga catatcgcga gcatcgcttt
     1861 gccgccggta gcggtggggc gaaagatcgc ttgaccgtca ccgtttcggg tttccatctg
     1921 gcgcggcgct cgatgtttat caccgaccgg cagattccgg ctggtttaca ccgcttcttt
     1981 tttgaaaaag gactagatat cgtctatttt caatgaccac gccgcatacg cgcctgtagc
     2041 tcaagtggat agagcatcag acttcgaatc tgagggttgg gggttcaagt ccctccaggc
     2101 gcgccaactt accgctactg cctcgctagt cgttatcgtc tgtttccaca cagtgtcctt
     2161 caggcgggaa aatgaatttc gtctttttgt gcaagcctca cgcttcatct ttgtagtgaa
     2221 agagtcgtag ttgttcgatg cctgataatg cagttgctgg atatcgaata gttgccggaa
     2281 agcatgttcg cacgctcaca acttccattc ttcgcacccg tctcaaccct ctccgactct
     2341 agctcgctga ctatcggccc gtaatccatg cgcctttgcg cccgtgcgct tttttaaatc
     2401 ttctacctgt atatttcgct aggattagtt cttgattacc tatttgttgt gggacagaat
     2461 cgaaaagtca tcacctcgga gcctgcaatg ctgaaaacaa tttggaagtg gatcaagtgg
     2521 atgaaagagt ttactaaaaa atcaactcta gctggggctg ttttagctgt tagcggagtt
     2581 ctgggggtat cttgtaatta gatttttact caagcaacta ttgaatataa gcgacgacat
     2641 aagcgacatt atggatgagt ggagagatgt cggacgacat cagatattaa gttagggatt
     2701 ccggttgcga gcttcccgaa gtttccgaat agtaatagcg cgacaatcaa tgaaacatcg
     2761 cttacgtaag caaattgaag ttgtcgcggg attgattcta gctgagcaaa agttgctggt
     2821 ttgccagcgc catcgcagtg gcgcgtttcc atacaaatgg gagtttcccg gcggcaagat
     2881 cgaatcggaa gagtccgctg aagcggcgtt ggctcgcgaa cttaaagaag aactcgatat
     2941 tagcaccggt gagtttagct ttatttatcg ctgcaatcat gcctatgcca atggcctgga
     3001 tgtctcgctg agcttttatc gggtcatcaa ttaccaagga gttataaaaa atctagtatt
     3061 cgaacaaatc gcctggtcaa acttagagga actgacgaaa tttgattttc tcgaaggcga
     3121 cgtctccctt gtgcatcagt tggcaaacgg cgaattatcg gtcaactaac cgcggcgaca
     3181 aaaaattaac agatttgtga cgtattgtct gccgatcaat ttgacagccc accgcgccac
     3241 agttatccta acgccactag gatttcttta gtatctcatg aagagaataa tcataaccct
     3301 tgccgcggtt tgtctacttg ttaccgcggc tccgccggcg cgcgccgatg tttatatgtt
     3361 caaagacaag caggggattt tgaccttcac caacgtgcca aatcacgtag gctataagcg
     3421 aatacttaga gacagtgggg taacgctcgg cggtggcaat gtaaccggca cggcctttga
     3481 tgagttgatc agctccgcgt caggccgtta taacgtcgat ccggatttaa ttcgtgccgt
     3541 gatcaagacc gaatccgatt tcaacaccaa cgcgcgctcg cataagggtg ccatgggttt
     3601 gatgcaactc atgcctgaga cggcgcgcat gcataacgtc agcgatgcct acaaccccaa
     3661 tgaaaatgta gatggcggcg tgcgccatct acgaatgctg ctggaacgct accaaggcaa
     3721 tttcgagttg tcgctggcgg cttataatgc cggagctggc gcagtcgaga agcatggcgg
     3781 tattccaccg tttaacgaga cccgcgagta tgtccgcaaa gtcctgcggt tttacgatgc
     3841 ctttcgcgac accggtttaa attctttaca gcaagcgcgc cagtagcgct tttatttttt
     3901 cgagtagctt cttttgatgc cttatggttt ggacactacc gaacctcctt agtctatttc
     3961 gcattctgat tatcccgcca ttggtttatc tgctcagctt tcctggccgc agcacgtcgt
     4021 tatgggctgc cgtgttgttc ctggtggcat ctatcaccga ttacttcgac ggttacttcg
     4081 ctcggcgtaa caagagtgtc tccgatctcg gcaagatcct cgatccactt gccgacaagc
     4141 tcatggtcgc ctccgccttg atcatgcttg ccgccatgga ccgcccgggt gagcctagtg
     4201 tacccgcttg gttggttgtg gtaatcctcg cgcgtgagag cgccgtcacg gtgattcgcg
     4261 gtatcgcgct ctctgacggc atcgtcatgc aggctgagac cctcgggaaa tataagttca
     4321 tcatgcaagc ctatgcgatc gtcggccttc tcgtgcatta tcgatactgg ggttttgatt
     4381 ttttcgtcgc agggatgtac ttcctggtac tgtccgcagt aatcgcagca tggtccggcg
     4441 tcaattatca tgtgcagttc tttcgcctgc gactggtccg cagcgcccgg taattttttt
     4501 tgcgaaattg acaaagccga aaccgtcatg tagaaattta attgatgcgg gtgtagctca
     4561 gttggctaga gcgtatgctt gccaagcata aggtcgtcgg ttcgaccccg atcacccgct
     4621 ccagtttcaa gcccttggga agccaagggc tttttttccg atgcgcttct ctcaaacctc
     4681 tgataatcct ggcgagatat catgtcgcac tattttcgca tgttttcgag taacgcttcg
     4741 aaggaatgcg ccgcagtcca tctcagtagg ggcctccgag ggaatcttcc tgcggtattg
     4801 atcgagtctc cgctcgttcg acagaaagat cgaagagacc ggaccggagt tccgcatgac
     4861 ggtaatcaaa cgactgtgag cggatccgaa gccggcgcag catcggcacc ggcaccacgc
     4921 ggaagaatgg ttaaacctcg gttcatcgat cggcgccgta gcgtgaggcg tggatatgta
     4981 gcatttatcc gacgtagttg tcccggattc cgcagtagaa gattcaaggc gactgactga
     5041 cagtagagag cggagataac agcggaatcg gggaaatgat tttaacggga tgagtcggag
     5101 tttggttggg cagactccat tccaactgcg ccgcaatcgg caatccctcc gacttcgttc
     5161 gcaaacagcg caagagctcg ggtcggtcag gctctgggat ggcaaaccga actctgaccc
     5221 ggcgtccctg gtggatcaga tcggcaccca ccaaaaagac aaaatccctg accgctctga
     5281 ggcgcttctc aaaccacgag cgtggcaaca ccgtctctcg aaagtccgcc agtagattgt
     5341 acagcacacc gcccatgcga aaggctgcat cggtggcgtc aaaggacttc aaacaaaagg
     5401 tgtccaggct caggtcttct tttaactcct tgatccggtt ctcgctgtcg gccctgccgg
     5461 catacatccg agtaacgatt tcagcggcat agggcatgct ggtcaccatc accctatagc
     5521 tatatcccgg ataatcgatt aaccgtctcc cactggcttg gggccgctct gaaatttctt
     5581 ggcgcaggca aacaaagcgc cgcttgaccg ttttccagtt cggtagactg acttctaagt
     5641 cggcgacatc gatcccccgg gccactgccc gccacgcagt agccgggatc tgatgaacca
     5701 ccaacttgcg caccaccgcc gttaaccggg caacaataat gtagggcaga tcctggttct
     5761 ccagatactc caacaaacgc ttctcaaaaa aaacctgcat cggctcgcac caatccgatc
     5821 cgttgcccat cgggcaacac cgtcagcgat tgcttcaaaa actctacaca gccgttggca
     5881 ctgccactat tcccactgcg caacgtcgcc cacaacaacc ggcgcttttc ggctaaaaac
     5941 cctaccaacg gatgatccga cggccgccca ggcttcttag gattgtaacc cttcaagctc
     6001 ccttcttgtt cgccgtaacg acaaaggatt gtagaatcca gatccaacgt atgacccatc
     6061 aagatcggcc gcatcctagt caaactaaac cgcatcagcc gctcgctcac ctcggtgact
     6121 tcccgatacc caaaacgcaa aaacagccgc ctcaatgtat ccggtgaacc aaaccg
//
LOCUS       DASQKS010000393         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6102929, whole genome shotgun sequence.
ACCESSION   DASQKS010000393 DASQKS010000000
VERSION     DASQKS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6102929"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1355
                     /locus_tag="VE170_10205"
     CDS             273..1355
                     /locus_tag="VE170_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ15851.1"
                     /translation="MISIRALQKSFNAQQGRVDALRSINLEVAEGEFCVLLGPSGCGK
                     TTTLRCVAGLERPDGGEIEIAGKLVNSPARRVFVATERRELGMVFQSYAIWPHMNVFQ
                     NVAFPLTQGRTRHSKADVADRVEKALKRVQLNGLGERPATDLSGGQQQRVAMARAMVT
                     EPKILLMDEPLSNLDARLREQMRVELRKITKSIGVTTLYVTHDQAEALSLGDRVCVMN
                     AGEILQIAPPHDVYEKPASLFVAQFVGEMNFVKGTVEAKDRVASPLGAIAVAVPDGIH
                     AGAAVTLAIRPEHVQVSAACRDNVASTFGIVNSKNYLGDAALLEVEVNGVTLLAKLAG
                     DTEVSVGQQAAVALPAHRWQVFGELG"
     gene            1546..3603
                     /locus_tag="VE170_10210"
     CDS             1546..3603
                     /locus_tag="VE170_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYJ15852.1"
                     /translation="MATFTAKPVGNLLRAKSVAIIGASQKGAWPQGIYRNLKGAKFPG
                     GVYLINPNYQELYGDKCYPSLTACPEVPDELLILIPTRAVLGVLEEAAQLGTRSATIY
                     TAGFGEGDDEKGKERAQQMRELCDKTGMVICGPNCMGSYSVGEGLWTFPTATPLLKKG
                     PVGLIFQSGGSLGNWIKGATERGIGFSYAISSGNEVSLDLVDYLSFLVDDPDTKVITL
                     MIEGIRRPEAFMAVAQQALQKGKPILAVKLGRSEMGKRQAISHTGSLAGSDEVFDAMC
                     KRLGIIRCPTLEDMTELTLAFMLGRFPKGGRAAIVVNSGGMKGLICDHCDDLSTNLAQ
                     LSDKTKEAVRPLIPAELAVENPLECGVAGFGDEAGFINIVKLHAEDPGVDLMAIHGEL
                     PRGQTRNAGLFKSVLEATDKPVLAFGRSTYSCTDESRAFQEEAGMPFLQAIKPTLRAL
                     AGLGLYGERRSAGVPTLPAPNGNPAVLDGEMLHKLLQSKGIGLPKQALADSAAEAAAR
                     AKEIGFPVALKLIAADVVHKTESGAVVLGLKSGEEVQAEGQKLLSKTSGKAQLLVQEM
                     VQGTEVLIGARTDPQYGPFLMVGLGGIFVEVLKDVSIRLLPVDDKEARAMLKELRGFK
                     VLEGVRGQGPRDVDALVKAMVGLSEIFATHRNSLSDMEINPIMVRAQGGGVAAVDVRL
                     VRK"
BASE COUNT          785 a         1025 c         1202 g          787 t
ORIGIN      
        1 acagtggtgt ggcagttttg gggcagcggc tgggtgccgc aagtggccgc gctgggcgtg
       61 attttgattc ttttcgccgc catcgtcgtc ggcgccgtgc gcttgatcct atcgcgtatc
      121 ggcgagatcg gcgaggccgc atagcgcgag cgctacgcga ttccaaattc aatattccag
      181 attccagatg ggatacttaa ggcgtgacgg cagttcgaag gatttggaat atggaatctg
      241 taatttgcaa ttccgaacgc cagtgaggtc ccgtgattag catccgtgct ttgcagaaat
      301 ctttcaacgc gcagcaaggg cgcgtcgacg ccctgagatc catcaacctg gaagtcgccg
      361 agggcgagtt ttgtgtcttg cttgggccga gcggttgcgg caagactacg accttacgct
      421 gcgttgccgg gttggagcgg cccgacggcg gcgagatcga gatcgccggc aagctggtca
      481 attcgccggc gcggcgcgtt tttgtcgcga ccgagcggcg cgaactcggc atggtgtttc
      541 aatcctacgc catctggccg cacatgaacg tgtttcaaaa tgtcgccttt ccattaactc
      601 aggggcgcac gcgtcattcc aaggcagacg ttgccgatcg cgtcgagaag gcgctcaagc
      661 gcgtccagct caatggcctg ggcgagcggc cggcgacgga tttgagcggt ggccagcagc
      721 agcgcgttgc gatggcgcgc gccatggtga ccgagccgaa gattctcttg atggatgagc
      781 ccttgagtaa tctcgacgcg cgcttgcgcg aacagatgcg cgtggaattg cgaaaaatca
      841 ccaagtcgat cggcgtgacc actctctatg tcacccacga ccaggctgaa gctctgagtc
      901 tcggcgaccg cgtctgcgtg atgaacgccg gcgagattct tcaaatcgcc ccgccgcacg
      961 atgtttacga aaagccggca agcctgttcg tcgcgcagtt cgtcggtgag atgaattttg
     1021 tcaaaggcac cgtcgaggcg aaggatcggg tggcgtcgcc attgggcgcg atcgccgtgg
     1081 ctgtgccgga tggtatccat gccggcgccg ccgtgaccct ggcgattcgg cccgagcatg
     1141 tgcaagtatc cgcagcgtgc cgtgataatg tcgccagcac cttcggcatc gtgaacagca
     1201 agaattatct cggcgacgcc gcgctgttgg aagtagaagt gaacggcgtg acgctgcttg
     1261 ccaaactcgc cggcgatacg gaggtgagcg tgggacagca ggcggcggtc gcgctaccgg
     1321 cgcaccgctg gcaggtgttc ggggagttgg gttgaggtag ccgcaaagaa cgccaaggtc
     1381 gcaaacaaat tttgcgataa atttcgcaca gccgctgcgg aagtgatcgg cgctcgatcg
     1441 tcaaatcttt actttccatt gctccaacac tccgaccctg ccgattcttg cagtcatcct
     1501 gcgtctatga catagcaaac ccatctcaat ttagaaggaa attaaatggc tacattcact
     1561 gcgaaaccgg ttggaaatct cttgcgcgcg aagtcggtgg cgatcatcgg cgcgtcgcag
     1621 aagggcgcgt ggccgcaggg gatttatcgc aatttgaaag gcgcgaagtt tcctggcggc
     1681 gtatatttga tcaaccccaa ttaccaagaa ctttatggcg acaagtgtta cccgagcttg
     1741 acggcatgcc cggaagtccc cgacgaattg ctgattctca ttccgacccg cgccgtactc
     1801 ggcgtcctcg aagaagcggc gcagttgggc acccgttcgg cgacgattta caccgccggc
     1861 ttcggcgaag gcgacgatga gaaaggcaaa gagcgggccc agcagatgcg cgagctatgc
     1921 gataagaccg gcatggtgat ctgcggtccc aactgcatgg gttcctattc cgtcggcgaa
     1981 ggactgtgga cgtttcccac cgcgacgccg ttgttgaaga aaggtcccgt tggcttgatt
     2041 tttcagagcg gcggttcgct cggtaactgg atcaaaggcg cgaccgagcg cggcatcgga
     2101 ttctcatatg cgatttcgag cggtaacgaa gtaagcctcg acttggtcga ttatctttcg
     2161 ttcctcgtcg acgaccccga cactaaagtc attacgctga tgatcgaggg cattcgccgg
     2221 cctgaggcgt tcatggcggt ggcccaacag gcgctacaaa aaggcaagcc aatcttagcc
     2281 gtgaagctcg gccgttcaga aatgggcaaa cgccaggcga tctcccacac cggttccctc
     2341 gccggctccg atgaagtatt cgacgccatg tgcaagcgct tgggcattat tcgttgcccg
     2401 acgttggaag acatgaccga gctaaccttg gcgttcatgc tcggaaggtt tcccaagggc
     2461 ggccgcgcgg cgatcgtcgt caactccggc ggtatgaaag ggctgatctg cgatcattgt
     2521 gacgacttga gcaccaacct ggcgcagctg agcgacaaaa ccaaagaagc ggtgcggccg
     2581 ctgatcccgg cggaattggc cgtcgaaaat ccgcttgaat gcggggtcgc cggtttcggc
     2641 gacgaggcgg gattcatcaa catcgtcaaa ttgcacgccg aagatcccgg cgtcgatctc
     2701 atggcgatcc acggcgaact gccgcgcggc cagacgagga atgccggctt gtttaaaagc
     2761 gtgctcgagg ccactgacaa gccggtgctg gcgttcggcc gttccaccta cagctgcacg
     2821 gacgaaagcc gcgcctttca ggaagaagcg ggcatgcctt tcttgcaggc gatcaagccg
     2881 acgctgcgcg cgctggcggg attgggtttg tatggggagc gcagaagcgc cggtgtgccg
     2941 acattgccgg cgccgaatgg taacccggcg gttcttgacg gcgagatgtt gcataagctt
     3001 ttgcaaagca aaggcatcgg cttgccgaag caagcgttgg cggactcagc ggccgaggca
     3061 gccgcgcggg caaaggagat cggctttccg gtggctctta aattgatcgc cgcagatgtc
     3121 gtgcataaga ccgagtccgg cgcagtggtg cttggtttga agagcgggga ggaggtacaa
     3181 gccgaagggc aaaaactgct ttccaagact tcgggcaaag cgcagttgct cgtacaagaa
     3241 atggtccagg gaactgaggt acttatcggc gcgcgcaccg atcctcagta tgggccgttc
     3301 ttgatggtcg gtctcggcgg catcttcgtc gaagttctga aagacgtgtc gattcggttg
     3361 ctgccggtag acgataagga agcacgtgcc atgttaaagg agctgcgcgg cttcaaggtt
     3421 ctcgaagggg tgcgtggcca gggcccgcgc gatgtcgatg cgctggtcaa ggcgatggtc
     3481 ggcctgtcgg aaattttcgc cacgcacaga aattctctgt cggatatgga gatcaatccg
     3541 atcatggtaa gggcgcaagg cggcggtgtg gcggcggtgg atgtgcgatt ggtgcggaag
     3601 taggaaaatc ggccgcaaag aacgccgacg gcgcaaagag agattggttt ctacaggacc
     3661 gccggtttga tcttctgccc gtgtaggaac ctccgcgctt tttcaattcg aaagtcgcat
     3721 tgcgccgatt gcgctatttc agcttcttca aaaacccttc atcgtcgatg gctttgatta
     3781 ggcttgggtc gaccatcga
//
LOCUS       DASQKS010000394         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3576547, whole genome shotgun sequence.
ACCESSION   DASQKS010000394 DASQKS010000000
VERSION     DASQKS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576547"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VE170_10215"
     CDS             complement(<1..948)
                     /locus_tag="VE170_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYJ15853.1"
                     /translation="MSPFSLTFFLTLLLVGHASFSVRAADAQNTPFSVPAGLQDAVNF
                     WQQVFTRYGTGEVILFDPLDPGKIYGVVRAAETEQGRALVSTLRARIAAEYDLADDEM
                     RVRSQRGAKEHFTEGLRISGRYISQMQKIFRDEGLPSELAYLPLVESSFNVRARSTAG
                     AVGMWQFIPETGKKFMRIDNAVDERRDPTASTRAAARLLKENYRIFRSWPLALTAYNH
                     GTEGIFRGIESVGSRDLVELIRQYRSPTFGFASKNFYAEFLAVIEIAKRPEKYFPFLR
                     LHQPVVLRTVEVKKSTVLDAVLRPAAISHTTFFQWNPALE"
     gene            1020..2546
                     /locus_tag="VE170_10220"
     CDS             1020..2546
                     /locus_tag="VE170_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15854.1"
                     /translation="MPRYFNRSCWLIVLIYLAAGCRWSGQERRPNYLVLGIEANPAQL
                     DPRYATDANAVRIGHLIYNSLLRADEKMQLQAELAENWQRLGERTFQFDLRENIRFHD
                     GRPLTAADVKFTYESIADPATRSPKRALLRQLEAIELAGSHRLRFHLKEPHAPFVEHF
                     AIGIVPRGSPSDTRPPPGSGPFELESFVSGEKVILKANANYWERKPALDGLVFKIVPD
                     AMVRALEFKQGAIDFMQNDLEPDLLPWLQSQTDAEVSSHQGTTFQYIGINLTHPILQQ
                     LKVRQAVAHAIDRPRIIRHLLKDAAVAANGLISPLNEAYDGKVTEWPYDPAKAKQLLD
                     DAGFKDPDGDGPRARFRLSYKTTNIDLRRRIAEALKEQLLQVGIELEIRSYEWGTFFS
                     DVKKGNFHLYSLAWVGIRDPDVQHQIFHSASVPPNGDNRGRYANPQVDRLLDRGRITV
                     EPVERKRIYGAAQRLIADDLPYVPLWWQKNVIAKKSYVQGFIPYPDGDLISLKQVSLR
                     "
     gene            2556..3479
                     /locus_tag="VE170_10225"
     CDS             2556..3479
                     /locus_tag="VE170_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ15855.1"
                     /translation="MKKFIWRRLLLLLPTLFGALSLVFLLVHLIPGDPVEVMLGETAS
                     GADKQELRRALGLDKPLATQYRTFLTKLAAGDLGSSLFEQASVSEIIGQRLPATLELA
                     LCAMIAALAISFPLAFLAARRPGSSADRMALLFSLLGLSLPNFWLGPLLMIVFSIQLG
                     WLPVSGRGGPLHVILPAFTLGLGMAAILTRMLRASLLQVARTEFVQTARAKGLSEKSV
                     WLKHMLRNALLPVVTIMSLQFGALLAGSLITETVFSWPGIGRLTVQAIQTRDYPLVQG
                     CVLVIAVAYVLVNFFTDIIYKWVDPRVSYGD"
     gene            3469..4269
                     /locus_tag="VE170_10230"
     CDS             3469..4269
                     /locus_tag="VE170_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ15856.1"
                     /translation="MATRVWFNTGAAILMVLLVAALTAPVIAPHNPLRQDLENDLIAY
                     SAAHPLGTDKLGRDILSRTLYGTRISLSVGFATVAISLAVGFVVGALAGYFGGWIDLM
                     LMRLVDILLAFPGILLAIAFTAVLGPGLDHVILALCLIGWTGYARLVRGEIIALRERE
                     FIQAARALGGSPKRVVFHHLLPNLLAPLLIQATFGLAAAIIAEGSLSFLGLGVEPPTP
                     SWGSMLNDGRQFLLVAPHLTTYPGLAIMITVFALNLVGDGLQERLERR"
     gene            4475..4648
                     /locus_tag="VE170_10235"
                     /pseudo
     CDS             4475..4648
                     /locus_tag="VE170_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            4670..>4975
                     /locus_tag="VE170_10240"
     CDS             4670..>4975
                     /locus_tag="VE170_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ15857.1"
                     /translation="MPRNQLDRAKVRIWVDFFNTRIHPTGSDLQKKRDPEKAQVRMDE
                     HLRSLDDALAGKDYLVGNQFTLADVTFIPFYTRRDRYTVAFDDYPNIKRWAEKLIARP
                     "
BASE COUNT         1055 a         1512 c         1345 g         1063 t
ORIGIN      
        1 ttctagggcc gggttccatt ggaaaaaagt ggtgtgagat atcgccgccg gccgaagtac
       61 ggcgtccagc actgtgcttt tcttgacttc gaccgtgcgt agcactaccg gctgatgcag
      121 ccgcaagaat ggaaagtatt tctcgggacg tttggcgatc tcgatcaccg cgagaaactc
      181 ggcgtaaaaa ttcttcgacg cgaaaccgaa agtcggcgat cgatattggc gaatcagttc
      241 gaccaagtcg cgcgagccga cgctctcaat gccgcgaaag atcccttccg tgccgtgatt
      301 ataggccgtc agcgccaacg gccaactgcg aaatatacga taattctctt tcaacaaccg
      361 cgccgccgct cgggtcgacg ccgtcggatc gcgccgctcg tccacggcgt tgtcgatgcg
      421 cataaacttc ttgccggtct caggaatgaa ctgccacatg ccgacagcgc cggcagtgga
      481 acgggcacga acgttaaacg acgactcgac cagcggcagg taagccaatt cagaaggcag
      541 cccttcgtca cgaaagattt tctgcatctg agagatataa cggccggaga ttctcaaccc
      601 ctcagtgaaa tgttccttgg cgccccgctg actccgcact cgcatctcgt cgtcagccag
      661 gtcgtactct gcggcaatac gtgctcgcag cgtgctgacc aaagcacggc cttgctcggt
      721 ttcagccgcc cgcaccacgc cgtagatttt tcccggatct aacggatcga acaggatcac
      781 ttcgccggta ccatagcggg tgaaaacctg ttgccaaaaa ttcaccgcat cttgcaaccc
      841 agccggcacc gaaaacggcg tgttctgggc gtcagccgca cgcacgctaa aagacgcatg
      901 gccgaccagt aggagagtga gaaaaaatgt taaggagaat ggagacattg gcgcttgttg
      961 aaaattgacc gatagtagca aacaagctag agtcagtcaa aattcgtttg agattcgcct
     1021 tgccgcgcta ctttaatcgc tcctgctggc taatcgtgtt gatttacctt gccgctggtt
     1081 gccgctggtc aggtcaggaa cgccggccca attacctcgt tctcggcatc gaagccaatc
     1141 cagcgcagct cgatccgcgc tatgctaccg acgccaacgc cgtgcgtatc ggccatttga
     1201 tttataattc gctgctgcgc gccgacgaga agatgcaatt gcaagccgag ctggccgaga
     1261 actggcagcg gctcggcgag cgcacttttc aattcgatct acgcgaaaac atcaggtttc
     1321 acgacggccg gccgctgacc gcggcggacg taaaattcac ctacgaatcg atcgccgatc
     1381 ccgccacccg ctcgcccaag cgtgcgctgc tgagacaact tgaagccatc gaattagccg
     1441 gcagccatcg gcttcgtttc catttaaaag agccccatgc gcccttcgtc gaacacttcg
     1501 cgatcggcat cgtgccccga gggtcgcctt cggacactcg tccgccgcct ggctcgggac
     1561 cgtttgaatt agagagcttt gtgtccggcg aaaaagttat cctgaaggcg aacgctaatt
     1621 actgggagcg caagcccgcg ctggacggcc tggttttcaa aatcgtcccc gacgccatgg
     1681 tgcgagcctt ggagttcaag caaggcgcaa tcgacttcat gcaaaacgat cttgagcccg
     1741 acttgttgcc ctggctgcag agccagaccg atgcggaggt cagtagccat caaggcacga
     1801 cgtttcaata catcggcatc aatctcaccc acccgatttt acaacagctc aaagtccgcc
     1861 aagcggtcgc ccacgccatc gatcggccgc gcatcattcg ccatttgctc aaagacgccg
     1921 cggtcgcagc caacggcttg atctcgccgc tcaacgaagc ctacgacggt aaagtgacgg
     1981 aatggcccta cgacccggcc aaggccaagc agttgctcga tgacgccggc ttcaaagatc
     2041 ccgacggcga cggcccaaga gcgcgctttc ggctttccta caagacgacc aatatcgatc
     2101 tgcgccgacg catcgccgaa gcgttaaagg agcaactgct ccaagttggc atcgagctcg
     2161 agatccgcag ctacgagtgg gggacatttt ttagcgatgt gaagaaaggc aatttccatc
     2221 tctactccct agcctgggtc ggcatccgcg atccggatgt tcagcatcaa atctttcatt
     2281 cggccagcgt gccgcccaac ggcgataacc gtggccgcta cgccaacccg caagtcgatc
     2341 gtttactcga tcgcggccgg attaccgtgg agccggtgga gcgcaagcgc atctacggcg
     2401 cagcccaacg gctgatcgcc gatgacctgc cctatgtgcc tttgtggtgg cagaaaaatg
     2461 tcattgccaa gaagagctat gtccaaggct ttatccccta tcccgacggc gatctgatat
     2521 cgctgaagca agtctcgctg cgctgagcca aatccgtgaa aaaatttatt tggcgccgtc
     2581 tgctactctt gctgccgacg ttgttcggcg cgctgtcatt ggtgtttctg ctggttcacc
     2641 taattcccgg cgatccggtc gaagtcatgc tcggcgagac cgccagcggc gccgataaac
     2701 aggaactgcg gcgcgctctc ggtttggaca aacccctggc gacgcaatac cgaacctttc
     2761 tcacaaaact cgccgccggc gatcttggct cctcgctgtt tgaacaagca agcgtcagtg
     2821 aaatcatcgg ccaacggctg ccggcgactc tcgaactggc gttgtgcgcc atgatcgccg
     2881 cgctggccat atcgtttccc ctggcgtttc tcgccgcccg ccgtcccggc agcagcgccg
     2941 accggatggc tttgctgttt tcattgcttg gcctgtcgct gccgaatttt tggctcgggc
     3001 cgttgctgat gattgtgttt tcgattcagc tcggctggct gccggtgtcg gggcgcggcg
     3061 gcccgctcca cgtcatatta cccgcgttta cattgggcct gggaatggcg gctatcctga
     3121 cgcgcatgct gcgcgccagc ttactgcaag ttgcccgcac cgagttcgtc caaaccgctc
     3181 gcgccaaagg gttgagtgaa aaatcggtct ggctcaaaca tatgctgcgc aatgcgttgt
     3241 tgccggtggt gacgatcatg agtctgcaat tcggcgccct gctcgcaggc tctttgataa
     3301 cggaaaccgt attcagttgg cctggcatcg gcagactgac cgtgcaggct atccaaactc
     3361 gcgactatcc gctggtgcaa ggctgcgtgt tggtcatcgc cgtcgcctat gtgctggtga
     3421 atttctttac cgatattatc tacaaatggg tcgacccgcg ggtgagctat ggcgactaga
     3481 gtctggttca acaccggcgc cgcgatcttg atggtgctgt tagtcgcggc gctaaccgca
     3541 ccggtcatag cgccgcataa tccattacgg caagacttgg aaaatgacct gatcgcgtac
     3601 agcgccgctc atccgctcgg caccgataaa ctcggccgcg acatcttaag ccgcactctg
     3661 tatggcacgc gcatctcgct ctcggtcggc tttgccaccg tggcaatttc cctcgccgtc
     3721 ggcttcgtcg tcggcgcgct cgccggttac ttcggcggct ggatcgattt aatgctcatg
     3781 cgcctggtcg acatcctgct ggcgtttccc ggcattctcc tggccatcgc cttcaccgcc
     3841 gtgctcgggc ccgggctcga tcacgttatc ctcgcgcttt gcctgatcgg ctggaccggc
     3901 tatgcccgtc tggtgcgtgg cgagatcatc gctctgcgtg aaagagaatt catccaagct
     3961 gcgcgcgcct tgggcggcag tccgaagcga gttgtgtttc accacctgtt gcccaacctt
     4021 ctggcgccct tgttaatcca agcaacgttc ggcctcgccg ccgcgatcat cgccgaaggc
     4081 agcttgagct tcttgggcct gggcgtcgaa ccgccgacac catcctgggg atcgatgctg
     4141 aacgacggac gccaatttct tttggtcgcg ccccacctca ccacctatcc ggggctggcg
     4201 atcatgatca cggtgtttgc gctcaatctc gtcggcgatg gactgcaaga acggctagag
     4261 aggcgatagt tcaagccgtt caaatcgctc aatcgctacg ctgcgttcaa accgtatccc
     4321 gcggttttct gtctatttga acgacttgaa cgttttgaac ggcttgaacg agttgtatca
     4381 gattgcctaa ccccggccac gatgataatt agtctcacat caaggaggcc ccgcgatgaa
     4441 attatacacc agccacactt gaccctacgc ccataggaca aggattgtcc tggccgagaa
     4501 gaacatccct tacgaaaaga tcgaagtcga tctcaagaat aaaccggacg atctggttaa
     4561 acaaaatccc tacggcaagg tgccggtgct ggtcgacggc gacggtgtca tctacgagtc
     4621 ggcggtgatc aacgaatatc tcgacgagaa attcaacgat gcaccgctca tgccgcgcaa
     4681 tcaactcgac cgcgccaagg tgaggatttg ggtggatttc tttaacacaa ggattcaccc
     4741 gaccggcagc gacttgcaaa aaaaacgcga ccccgaaaaa gcccaggtgc ggatggacga
     4801 acacttgcgg agcctcgacg acgccttggc cggtaaagac tatctggtcg gcaatcagtt
     4861 caccttggcg gatgtcacgt tcatcccctt ctatactagg cgcgaccgct acaccgtcgc
     4921 gttcgacgac taccccaaca taaaacgttg ggcggagaaa ctgatcgcgc gccca
//
LOCUS       DASQKS010000395         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3576822, whole genome shotgun sequence.
ACCESSION   DASQKS010000395 DASQKS010000000
VERSION     DASQKS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576822"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VE170_10245"
     CDS             <1..571
                     /locus_tag="VE170_10245"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ15858.1"
                     /translation="RGRSSLKLYFKTDPARDYLNFHIIPREAISLSGIGDMLGFSCNL
                     LEQTNRGRLTLQSADPNDLPNIDPQVLEHPNDIAAMLAAMKFVQRLTSSEPLNEFFGD
                     LFSPDPKEDWEKFARSSYTSYYHGVGTCKMGPASDPMAVVDQRLRVHGMENLWVGDAS
                     IMPTVVHANTNFTVMMIAERAAEFIKAQS"
     gene            722..1750
                     /locus_tag="VE170_10250"
     CDS             722..1750
                     /locus_tag="VE170_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15859.1"
                     /translation="MKRILFVLALVFVVPVNASAQAPFYQDKTIRIVAGYGAGSVDDA
                     WTRLIARYLAKHIPGNPNMIVQNMPGAGAMIAANYVYKVAKPDGLTLGGIRSGLYFDQ
                     LVGRQQVQFDWPKFTWLGSPTQVEQLIYIRANTPYKTIADVRKAAVPPKCGATGTSST
                     GYYTGNLLEETLGAKFQTLTGYKDGPDIELAVERDEVQCRGISIETLFGREPLIGWHK
                     SGFIRVLIQTGSKRDPRLADVPTIWELMSEHKTPDAGIRLAKIILAVGAFGRPYVSSP
                     GLPPERAKIIQGAFKKTITDPDFQAQAKERKLEIDPVGGEELAALAREVIAQPTEVVE
                     RMKRLLEK"
     gene            complement(1752..2717)
                     /locus_tag="VE170_10255"
     CDS             complement(1752..2717)
                     /locus_tag="VE170_10255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15860.1"
                     /translation="MKRLASVVFLFSALYAPLAGFGAAPRSGAGEKVIIGLPTTSMSM
                     LPVFFAHDKGFFREEGIEPQLVFVGGRLQVPALAAGEVDFSASAETALRAAVQGLPLK
                     VVVYMNSRMAVSLMAAPEIKTIAGLKGRSIAVTSIGGSLDYAAREILAKNGLNPERDA
                     RFIGIAQSEQMIALSAGSIHAAMLTPPFDAMMEKKGFRRLVFVGDVLDYPQGGLATTD
                     KKIQENPAQVKRVVRTMVRSLIRIRQNRDELVGYIAQRWKMAPEFAAASYDTMLKAYS
                     PDGMTSAQSIQSVIDSIKGRNPAVGISQVVNFASLEEVQREMKLK"
BASE COUNT          648 a          893 c          792 g          566 t
ORIGIN      
        1 gcgcggccga tcaagcttaa agttatactt taagactgac ccggcgcgcg actatcttaa
       61 tttccacatc attccccgcg aagcgattag tctgtccggt atcggcgaca tgttgggctt
      121 ctcgtgcaat ctcttggagc aaaccaaccg cggccggctc accctccaaa gcgccgatcc
      181 aaacgatctg ccaaacatcg atccgcaggt cttggaacac cctaatgaca tcgcggcgat
      241 gctagcggcg atgaagttcg tccagcgcct caccagcagc gagccgctaa acgagttttt
      301 cggcgactta ttctcgcccg accctaagga agactgggaa aaattcgcgc gctcgtcgta
      361 cacgagctac taccacggcg tcggcacctg caagatgggc ccagcctcgg accccatggc
      421 cgtggtcgat caacgattgc gcgtgcatgg catggagaat ctctgggtcg gtgacgcctc
      481 gatcatgccc accgtggtcc acgccaacac caatttcacc gtcatgatga tcgccgaacg
      541 ggccgcggag tttatcaagg cgcagtcgta aaaaaacgct gcggcggcac gagaaccata
      601 agaattcgcg cattgcgaac ttctggtctc tgcgtgaggc ccagcctcac gtctcgctct
      661 tgtaagtttc ccggcaacct cgctatgatc gccgccgtca agtcacctac gggaggctgt
      721 catgaagcga atcctgttcg ttctggctct cgtcttcgtt gtgccggtta acgcatcggc
      781 gcaagcgcca ttctaccaag ataaaaccat acgcatcgtc gccggctacg gcgccggcag
      841 tgtcgacgac gcatggacgc gattgatcgc gcgctatctc gccaaacaca ttcccggcaa
      901 tccgaatatg atcgtgcaga acatgcctgg cgccggcgcg atgatcgcgg ccaactacgt
      961 ttacaaagtc gccaagcccg acggcctcac gcttggcggc attcgcagcg ggctatattt
     1021 cgaccaactg gtcggcagac aacaggttca attcgattgg ccaaagttta cctggctcgg
     1081 cagtccgacc caggtcgaac agctgattta tattcgcgcc aacacgccgt ataaaaccat
     1141 cgccgacgtg cgcaaagctg ccgtgccgcc aaaatgcggc gctaccggga cgtcgtcgac
     1201 cggttactat accggcaacc tgttggagga aaccctcggc gcaaaattcc agacgctcac
     1261 cggctacaag gacggtccgg acatcgaatt ggctgtggag cgcgatgaag tgcaatgccg
     1321 gggtatctca atcgagactt tatttggccg cgagccgctg atcggctggc acaagtcagg
     1381 tttcatccgc gtgctgatcc aaaccggcag caagcgcgac ccaagattgg ccgacgtgcc
     1441 gacgatctgg gagctgatga gcgaacataa gactccggac gcgggcatac ggctggcgaa
     1501 gatcatactc gccgtcggcg cgtttggccg tccctatgtc agctcgcccg gcctgccgcc
     1561 tgagcgggca aaaataattc aaggcgcttt caagaaaact atcaccgacc cggattttca
     1621 ggcgcaagcc aaagagcgaa aactggaaat cgacccggtc ggcggcgagg agttagcagc
     1681 gcttgctcgc gaagtcattg cccaaccgac ggaagtcgtc gagcggatga aacgattgct
     1741 cgagaaataa tctatttgag cttcatctcg cgctgcacct cttcgagaga tgcaaagttg
     1801 accacttggg agataccaac ggcgggattg cgccctttga tcgagtcgat cacgctttgg
     1861 atcgactgag cgcttgtcat gccgtccggg ctgtacgctt tgagcatggt gtcatagctg
     1921 gccgcggcaa attccggcgc catcttccag cgttgtgcga tgtatccgac caattcgtcg
     1981 cgattttgcc ggatccggat cagcgaccgc accattgtcc gcacgactcg cttcacctgc
     2041 gcaggatttt cctgaatttt tttgtcggtg gtcgccagac cgccctgtgg gtagtcgaga
     2101 acgtcgccga cgaataccag gcgccgaaag cctttctttt ccatcatcgc atcgaagggc
     2161 ggagtaagca tggcggcatg aatcgagccg gccgataaag cgatcatttg ctcgctctgc
     2221 gcgatgccga taaagcgcgc gtctctttcc ggattgagcc cgttcttcgc caggatttcc
     2281 cgcgccgcgt agtccaaact gccgccgata gaggtgacgg cgatggagcg tcctttcaag
     2341 ccggcgatcg ttttgatttc cggcgcagcc atcaaggaga cggccatccg tgaattcata
     2401 tacacgacaa ctttgagcgg caaaccctga accgcggcgc gcaacgcggt ttccgccgac
     2461 gcgctgaaat cgacctcgcc cgccgccaaa gccgggacct gcaaccggcc gccgacaaat
     2521 accagctgcg gctcgatccc ctcctcccgg aaaaatccct tgtcgtgggc gaaaaacacc
     2581 ggcagcatac tcatcgacgt agtcggcagc ccgatgatta ccttctcgcc ggcgccgctc
     2641 cggggcgccg caccgaaccc agccagcggc gcatacagcg ccgagaaaag gaacacaacc
     2701 gaagccaatc ttttcatctg aaaccttcca tcgatcgcat tgaccgataa gtcgctgtcc
     2761 atcagcaccg aaaataggcg acaacggcga gcggcgcaag gccgtcggat gcgcgggcag
     2821 tgccaatatc accgacaggt tcgatgccga acggccacgg attttaccaa agcgcgatcg
     2881 tgtcacaata acggagata
//
LOCUS       DASQKS010000396        11488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2740084, whole genome shotgun sequence.
ACCESSION   DASQKS010000396 DASQKS010000000
VERSION     DASQKS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740084"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VE170_10260"
     CDS             complement(<1..850)
                     /locus_tag="VE170_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15861.1"
                     /translation="MRWIIQWACGVMISIGMLAPSPGTFAAETALKVVDVSLPAVSLL
                     QSPLFAAIESGAFKKYGMEVRYIVTGARSIQALIGGSVNFAQGVSSRTVPSAVLAGFD
                     AVLIANFSDKFLFTMHSAPEINSLQDLKGKIVGVSGLGGSTDFATRVALREAGLVPDK
                     DVMIRGVGGVPETVAALRAKLVHAGTLSPPSSFVAQKAGFKMLFDMTSLGVDYVSSGL
                     GVKKSWLVANRLEAKQFVMAMIEGAKILSTDEEFSLRVLAKHTRISDREVLQQSYRYI
                     KPYFLKV"
     gene            complement(933..1514)
                     /locus_tag="VE170_10265"
     CDS             complement(933..1514)
                     /locus_tag="VE170_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15862.1"
                     /translation="MKEFWFIGALLFGVYYWSGREIARPGGVLVPEEPVQHQVYTSRL
                     REKNGFQIAILATFEIRARVIAVESYRFDRGAALSPVDLALGWGPMSNGDVLKQISFS
                     QGGRFYSWWTRKFPVPRNVIESHSANMHMIPADDDIERRLKSTRVGNLVRIKGYLVEA
                     TSKEGFRWKSSLSRSDTGAGACELIWVESLESW"
     gene            2144..2959
                     /locus_tag="VE170_10270"
     CDS             2144..2959
                     /locus_tag="VE170_10270"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HYJ15863.1"
                     /translation="MAKYALCIGINNYPGTDSDLSGCVNDANDWAAALTKRGFTTTKM
                     YDKKASGKNMRAAIKSVIGAAGAGDLVVIQYSGHGSYVPDDNGDEPDGKDECLCPYDI
                     YTNGPITDDELFEMYSAKKTGVKLLVISDSCHSGSNAKFAPITTPPTIKGRKAPQRKV
                     RFLPPAAFLSKRELAKLGSSRAIRRSSPPGRHAGLLMAGCQDSEYSYDASFQGRPNGA
                     FSFVALRELAKLPASANYQRWYDAIRKALPSQQYPQTPNLYGTKTMKTWKIFV"
     gene            3286..4125
                     /locus_tag="VE170_10275"
     CDS             3286..4125
                     /locus_tag="VE170_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYJ15864.1"
                     /translation="MAKAKFASGGARGTALFLKEGSTGPAVAKLQAKLKEVGFNPGRP
                     DGDFGPATTAALIGFQKSAGLLADGIAGPRTLTALNLAASDRLPSVIPGVTVNIVSKM
                     FPFTRVDNIRDNLPFVLKGLVERMLGDKPMVLMALPTIRAETESFQPIEEGLSRFNTS
                     PGGHPFDLYDFRSDLGNRGKGDGAKYRGRGYIQLTGRFNYQKYGAALGMGNKLATDPE
                     LANDPAIAGNLLAAFLKDKERALKEALIIGDLRLARRLVNGGSHGLDRFTEAFRIGDS
                     LIV"
     gene            4177..6267
                     /locus_tag="VE170_10280"
     CDS             4177..6267
                     /locus_tag="VE170_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HYJ15865.1"
                     /translation="MPKRTLEIAGNKITLDARPDRLDIRDLPYRPAVVSFPPIYPTDK
                     DIARLLSNYIAAGLILDQKSEGACTGFGLAAAINFLLWRRSQHKLKKKDRVSQRMLYH
                     LAKFYDEWPGEDYQGSSCRGALKGWQRHGVCRDSLWPYKDKEFVRPLAGWDSDALCRP
                     LGVYYRVDRQSVVDLQAAIFDSGAVYVSANVHAGWEVKPYKGTIKHRTLPVIEASAVG
                     YGGHAFALVGYNEIGFIVQNSWGEKWGARGFAVLPYEDWAVNGTDAWVMGLGAPVQRL
                     QQRQYFVKHNDGIAGSAARSALIGPARAKSKTTAETWSEQETYWHTIVTGSDVQIINR
                     LPHVRDEKDNVAWAAYEQPRQWFKKQSGGGKWRITVYAHGGLHSETSSIDRIRLLGPC
                     FSDNGVYPLFITWKNGWQEILGNMLADGASKLFGDKLAPSRGRGDTFREAADRALEVF
                     LRGALGKSVWSEMKENLMQSTGGGIAEIAAQLARLQKDSGNRLEIHLIGHSAGSFLCG
                     RLLAELRERNLKARSCTLFAPACDLKFALDHFKPAVDEGQLARSALRIHLLADRVERD
                     DAVGPYQKSFLYLVSRALERWHKTPLLGLVNAFDGDRATEEYWHSDTIKNSVKPWQQF
                     FWQGKVPHGFADEGSATPGGTLRILNAPEISCGPKNIKSSHGCFDNSVEIISGTIKAI
                     LGSELKRPITSLDY"
     gene            complement(6510..6773)
                     /locus_tag="VE170_10285"
     CDS             complement(6510..6773)
                     /locus_tag="VE170_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15866.1"
                     /translation="MTDNCDGTMRPLTRLLDTNDAEVLDFAYQYLHANSEATLYPPQD
                     AVKNLIRMAGYMDKKLASISVNRVMDLSLLDELGTKRHQRGQR"
     gene            6799..7281
                     /locus_tag="VE170_10290"
     CDS             6799..7281
                     /locus_tag="VE170_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15867.1"
                     /translation="MTNFFSVPRCPVAKERLTADWRVHERRRLAAQNKKSAFFHDRFE
                     QHVGKRGIDPAAHQRADPFYRAAAHLQRCEILGRIGTGVLQQHLDNQPGFTTGAIDAD
                     FFAAQVADVGDFFPRHPDIGHQIIPAAQRDHIRALRMARQQAGWIHDKFLRKIIGQFN
                     "
     gene            7373..8218
                     /locus_tag="VE170_10295"
     CDS             7373..8218
                     /locus_tag="VE170_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYJ15868.1"
                     /translation="MVPRPRDIGGFDVRRALPSAERRNVGPFVFFDQMGPAELAPGSG
                     IDVRPHPHIGLATVTYLFEGTIVHRDSLGSVQAIEPGAVNWMTAGRGIVHSERSDNEL
                     RKRRQKLSGIQIWVALPKQHEEIDPDFTHYAAGSLPRIEGEGKTVRIIAGSLFGKESP
                     VKTLSRLFYADAALDAGASLLLNNDYNERGIYLLEGDVDIHGQRFEPGRLLVFSSGDE
                     ITIKAVAAARFVLFGGEPLDSPRHLWWNFVSSSPERIEQAKSDWKSGRFAPVPGESEF
                     IPLPE"
     gene            8604..9542
                     /locus_tag="VE170_10300"
     CDS             8604..9542
                     /locus_tag="VE170_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ15869.1"
                     /translation="MFVTKQARSLAIFSAILLFEIILVSGAAGQKPGRSEAKTITLGI
                     VAETNQKEIEAHFQDFVGYLAKKLSSGSKVEGRIVIVSTQSRLASLLSARKADFYMES
                     PHPTHMVNNVYGAGKLLLRRWKGGMAEYHAVIFTAKNDGTKRLEDLQGKVIAFEDAES
                     TSGYFLPKLYLTKKGFRLVQKTQSDAKVSSGEIGYIFAGSQEKLVDAVLTNKVAAGAF
                     SNDDHAALDDSKKLQITVLAETASLPRHLVSVRKDLAPELAKGLEKALLSMHQDPEGR
                     SILQKADGTTKFDPLPGGELAVRQRLLDTYYSPQKK"
     gene            9749..10399
                     /locus_tag="VE170_10305"
     CDS             9749..10399
                     /locus_tag="VE170_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15870.1"
                     /translation="MKFKRRLQQLLLSALITSATSAMAATLFSEAWSDLTKYFTAAAS
                     LVLQASIGLDNTRFDTLPPAQRLEARTQLQKLKDQLNILFLEQSTLVSQFEFFVKIAR
                     DPSKTPAERKTYWDNNVLPRISTVSEAVSEVRTFSEKDGQPFSVVLSADDRLALGDTL
                     AARGIALKTFEKMAPPASADEVLQFQGLINDYNVLRQNLFKLRLAIDAALGRLASA"
     gene            10402..11250
                     /locus_tag="VE170_10310"
     CDS             10402..11250
                     /locus_tag="VE170_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYJ15871.1"
                     /translation="MAQLFISYASEDRNFVDSLAGGLTLAGHDVWFDRDLDAGPYREQ
                     ILARLTRAQVAIVVWSARSKQSRYVIDEAERAVSRGILLPIRTDDTDLPLGFGGLQTF
                     DLSSWSGRADDLQFTVLLDQIDKILKHPTVSRGRPAVPFIAKSLLLAGFAAAICAPAL
                     AWLYAVRNGSASTPHLYTILQAFVLAAVCTTPVMLWCGFEVGRFGLSHAAPILRRAIG
                     IYAIAAVVSLALVAAAVVGGVTTGRTPAAALGQIFFVAFPATLTVGVVITLLKATAYL
                     IKKLRM"
BASE COUNT         2544 a         3340 c         3184 g         2420 t
ORIGIN      
        1 gcaccttgag gaaatacggt ttaatatagc gatagctctg ctgcaaaacc tcgcggtcgg
       61 aaatcctcgt gtgcttggcc aacacgcgca gggagaactc ttcgtccgtg ctgaggattt
      121 tcgcgccttc gatcatcgcc atgacgaact gtttcgcttc cagccggtta gcgacgagcc
      181 agctcttctt gaccccaagt ccggaggaaa catagtcgac gccgaggcta gtcatgtcga
      241 acaacatctt gaaacctgcc ttctgcgcga cgaaggacga cggcggcgac agcgtcccgg
      301 cgtggaccag tttggcgcgc agcgccgcca cggtctccgg cacgccgccg acgccgcgaa
      361 tcatgacatc tttgtcgggc accagtccgg cttcgcgcag cgccaccctg gtggcgaaat
      421 ctgtgctgcc gccgaggccg gaaacgccga caattttacc ttttaaatct tgcagggaat
      481 tgatttccgg cgcgctgtgc atggtgaata aaaacttgtc gctgaagttg gcaatcagca
      541 ccgcatcgaa tccggcaagc accgccgagg gcacggttcg actcgaaact ccctgggcga
      601 aattgaccga gccgccgatc agcgcctgga tgctgcgcgc tccggtaacg atgtagcgca
      661 cctccatgcc gtacttttta aacgcgccgc tttcgatggc ggcaaacagc ggcgactgca
      721 ataacgacac cgccggcagc gaaacgtcga cgaccttaag cgccgtttcc gctgcaaaag
      781 tcccaggtga cggcgccagc atgccgattg aaatcatgac accgcaggcc cactggatta
      841 tccatcgcat cttcgctccg tgactaaact cgctatgcgc ggtttactcc aaggttggtg
      901 gaatgtctat gaatttgtca acacgccggc gtttaccaac tctccaacga ctcgacccag
      961 atcaactcgc aggcacccgc accagtgtcg ctgcgtgaca gcgagctttt ccagcgaaaa
     1021 ccctccttgc tcgtcgcttc caccaggtat cctttgatac gtacgagatt tcccacgcgg
     1081 gtcgatttca accggcgctc gatgtcgtcg tccgccggaa tcatgtgcat gttggcacta
     1141 tggctttcga taacattgcg gggaaccgga aatttccgcg tccaccagct atagaaacgg
     1201 ccgccttggg agaagctaat ctgcttcaaa acatcgccgt tggacatcgg tccccaaccc
     1261 aaagcgaggt ccacgggcga tagcgccgcg ccgcgatcaa accgatagct ttcgacggcg
     1321 atgacccgcg ctctgatttc gaaagtggcg agaatcgcga tctgaaaacc attcttctcg
     1381 cgcaaccgcg acgtgtaaac ctggtgctgg actggttctt ccggcaccag tacacctcct
     1441 ggccgggcaa tctctcgacc gctccagtaa tacacgccga aaagcagcgc gccgatgaac
     1501 cagaactctt tcattgcaat cgatctgcgc cgacagttta accaaagtcg gcaagccaga
     1561 gaagctaaaa gattgagcca ctaggttgct accggttaaa tttaggcccg taggcttcga
     1621 catcaacatt ggcctagaac aaccgaaaga gttcaccata gcgcagatat cccagcgcgg
     1681 atccgccacg accaaatgag aagaggttat cattacgaag gacctggcgc gcggcgcaag
     1741 tagtccgcat ttacctattg tcagaagaca cgcgaatatg taattacgga acggcaacga
     1801 tctagcgcat tttcatgagt gtctccctac tgtcattgat agagaattgc accagccggc
     1861 caacctatac ggttccggct aaaaattttt gcgatacgat atcgtcaagc gtctatggga
     1921 tacaccaaag taaccaatgg atacacccgc tgtatacgag agttaccggc ggccggcgcg
     1981 tctctctgaa tgtccgacaa taatcatgag attgcgccac gattgcgcgt ttttcagatt
     2041 tttatttaac cgctaacttg gcatcgccgt tgctgtaacc aatggctacg aaccgacaaa
     2101 ctggtaataa aaacttaata aaacgttgag gaggagaact tatatggcaa agtatgcgct
     2161 ttgtatcggt atcaataatt atcccggcac cgacagcgac ctttcgggtt gcgtcaacga
     2221 cgccaacgat tgggcggcgg cgctcacgaa acgaggcttc acgacaacca agatgtacga
     2281 caaaaaggcc agcggcaaaa atatgcgcgc cgccatcaaa tccgtgattg gcgcagccgg
     2341 ggccggcgac ttggtggtga ttcaatattc gggacatggc tcctatgtac cggatgacaa
     2401 cggcgatgag ccggacggta aagacgagtg tctgtgcccc tacgacatct ataccaacgg
     2461 accgatcacc gacgatgaac tcttcgaaat gtatagcgcc aagaaaaccg gcgtaaaatt
     2521 actggtgatt tccgactcct gccattccgg cagcaatgca aaatttgccc cgatcaccac
     2581 cccgccgact atcaaaggcc gcaaggcgcc gcagcgcaaa gtgcgcttct tgccgccggc
     2641 ggcgtttctg tcgaaacgag agttggcgaa gttaggttcc agccgtgcga tccgccgttc
     2701 cagcccccca gggcgtcacg ccggcctgct gatggccggt tgccaagaca gtgaatatag
     2761 ctacgacgct tcttttcaag gccggcccaa tggcgcgttt agcttcgtcg ctttgcgcga
     2821 gcttgccaag ctgccggcga gcgccaatta tcaacgctgg tatgacgcga tccgcaaagc
     2881 gctgccgtcg caacagtatc cccagacgcc gaatctgtac ggcaccaaaa ccatgaaaac
     2941 ctggaaaata tttgtctaac cacactttat ttcgctgcgc ttgtggtgtt gttaaccgcg
     3001 aaagaactgc cacacgaagg actcgaagat gcattaccgg acgaaagatt gttcgccctt
     3061 gcatcattcg aaccttcgtc tatttcctga tcatggtcgt tccgaataga cgaccaatca
     3121 aaagaagctt caccgcagcg tcacgaacgc tgcggagagt ttaactaaat tttgccaggc
     3181 ccctgctcac gacgatcgat attgacgatg gcgcatccgt cgaaccgaga aatgctaatt
     3241 tcggacaaga accaatagtg acaactctcg tgcaaggaga acctaatggc gaaagccaaa
     3301 tttgcgtcag gcggggcgcg cggcactgcg ctgtttctca aggaaggctc aaccggcccc
     3361 gctgtcgcca agctgcaggc taagctgaag gaagtcggtt tcaatcccgg ccgccccgat
     3421 ggcgatttcg gcccagctac caccgcggcg ctgatcggat ttcaaaagag tgctggtctg
     3481 ctcgccgacg gcatcgccgg accgcgcacg ttgactgcat tgaatttggc cgccagcgac
     3541 cggttaccct cggtgatccc gggcgtgacc gtcaacatcg tgtcgaaaat gtttccgttc
     3601 acccgggtgg acaacatccg cgacaacctg ccgtttgttt tgaaaggttt ggtcgagcgc
     3661 atgctcggcg acaagccgat ggtcttgatg gcgctgccga ccattcgtgc cgagaccgaa
     3721 agcttccagc caatcgaaga agggctgtcc aggttcaata cttcgcccgg cggccacccc
     3781 ttcgatctct acgattttcg ctcggacctt gggaaccggg gcaaaggcga cggcgccaag
     3841 tatcggggcc gcgggtatat ccaattgacc ggccgtttca attatcaaaa atatggcgcg
     3901 gctctaggca tgggcaacaa actcgcgacc gacccggaac tcgccaacga tccggccatc
     3961 gccggcaacc tgctcgcggc atttctcaag gacaaagagc gcgcccttaa agaagctttg
     4021 atcatcggcg atttacgcct ggcgcgccgg ctggtcaacg gcggcagtca cggtttggat
     4081 cgcttcaccg aggcgttccg catcggcgat agcctgatcg tctagcacaa cacgaaccat
     4141 ccctgcgcag cctgatccac ccgaaaggac gatttcatgc cgaaacgaac tttagaaatc
     4201 gccggcaata aaataactct cgatgcccgc ccggatcgtt tggatattcg cgatctgccc
     4261 tatcgccccg ccgtcgtgtc gttcccgccg atatacccca ccgataaaga catcgcccgg
     4321 ctactgtcaa attacatcgc cgccggtctg atcctcgacc aaaaatctga gggagcctgc
     4381 accggtttcg gtttagccgc tgcgattaat tttttattgt ggcgccggtc acagcataag
     4441 ttgaagaaaa aagatcgcgt gagccagcgc atgctgtacc atctggcaaa attttatgac
     4501 gagtggccag gcgaagatta tcaaggctcg agttgtcgcg gcgctctcaa gggatggcag
     4561 cgccacggcg tctgccgcga ctcactctgg ccttacaaag acaaagagtt tgtccgcccg
     4621 ttggcaggtt gggatagcga cgccctctgc cgtcccctcg gcgtctacta ccgcgtcgac
     4681 cgccaatcgg tggtcgatct gcaagcggca atctttgaca gcggcgcggt gtacgtttcg
     4741 gccaatgtcc acgccggctg ggaagttaag ccgtacaaag gcaccatcaa acaccggacc
     4801 ctgccagtca tcgaggcgag tgcggtgggt tacggcggcc acgcctttgc gctggtgggc
     4861 tacaatgaga ttggcttcat cgtgcaaaat tcctggggcg aaaaatgggg cgcccgcggt
     4921 tttgccgtct tgccctacga ggattgggcg gtcaacggca ccgacgcctg ggtcatgggc
     4981 ctcggcgcac cggtgcagag acttcaacaa cgccaatact tcgtcaaaca caacgacggc
     5041 atcgccggca gcgccgcgcg ttcggcgcta atcgggccgg cacgcgccaa atcgaagacc
     5101 acggcagaaa cctggtccga acaggaaacc tattggcaca ccatcgtcac cggcagcgac
     5161 gttcagatca tcaaccgctt gccccatgtc cgtgacgaaa aggacaacgt cgcgtgggcg
     5221 gcatacgagc aaccgcgcca atggtttaaa aaacaatccg gcggcggcaa gtggcgcatc
     5281 accgtctacg ctcatggcgg cttgcattcc gagacatcgt cgatcgatcg cattcggctg
     5341 ctcgggccct gctttagcga caatggtgtt tatccgctat tcatcacctg gaagaacggc
     5401 tggcaggaaa tcctcggcaa tatgctcgcc gacggcgcct cgaaactatt cggcgacaag
     5461 ctcgcgccga gccgcggccg cggcgatacc ttccgcgaag ccgcggatcg cgccttggaa
     5521 gttttcctgc gcggcgccct gggcaaatcc gtgtggtcgg agatgaaaga gaatctcatg
     5581 caatccaccg gcggaggcat cgccgagatt gccgcgcaac tggcacgcct gcaaaaagac
     5641 tcggggaatc gtttggaaat tcatctgatc ggccactcgg cgggctcatt cctgtgcggc
     5701 cgactgcttg ccgagctgcg cgagcggaat cttaaagcgc ggagctgcac gctgttcgcg
     5761 ccggcctgcg atctgaaatt cgctctcgac catttcaaac ccgccgtcga cgaaggccaa
     5821 ctcgctcgat ccgccttgcg cattcatctg ctcgccgacc gcgtcgaacg ggacgacgca
     5881 gtcggaccgt atcaaaagtc ctttctctac ctggtcagcc gggcactcga gcgctggcac
     5941 aagactcccc tgctcggtct ggtcaatgcc ttcgacggcg accgcgccac cgaagaatac
     6001 tggcacagcg atacaatcaa aaacagcgtc aaaccttggc agcaattttt ttggcagggc
     6061 aaggtacccc acggcttcgc tgacgaaggc agcgccacgc cgggtggcac tctgcgcatt
     6121 ctcaacgctc ccgaaatcag ctgtggcccg aaaaatatca aaagcagtca cggctgcttc
     6181 gacaacagtg tcgagatcat cagcggcacc attaaggcta tcctcggtag cgaacttaag
     6241 cgtccgatca cgagcctgga ttattgagtt cggacgtctt cccagagaca agaaaagacg
     6301 gatcacaaat tcgtcgctga tacgacccga tcgcaaccgg tgaatctgct ggcgcgggca
     6361 ccggccgagg ttgatctacg agcgccgcaa agcaatcgtc atatagaagg aatattcata
     6421 ggcagaagcc cgtgatttca cgtcaagtag atcaagcgca ggtgcgaggc cttgcgacaa
     6481 cttatgtctt cggagcgaat tcttgaccgc tagcgctggc cgcgttggtg acgtttggtc
     6541 cccagttcgt cgagcagcga gaggtccatg acccgattga cactgatgga cgccaatttc
     6601 ttgtccatgt agcccgccat gcggatgaga ttcttcaccg cgtcttgagg cggatagaga
     6661 gtcgcctcgg aattggcgtg caaatactgg taggcgaagt cgagaacctc ggcgtcgttg
     6721 gtgtcgagca gtcgggttaa agggcgcatg gtgccatcgc aattgtcggt catgtagcgg
     6781 gtcgcgtcgg cgtaggcttt gacgaatttc ttcagcgttc cccgctgccc ggtggcgaag
     6841 gaacgcttga cggcggattg gcgcgttcat gaacggcgtc ggctcgccgc gcaaaacaaa
     6901 aaatccgcgt tttttcatga ccggttcgaa cagcacgtcg gcaagcgtgg catcgatccc
     6961 gccgcgcatc agcgcgccga tccgttctat agagccgccg cccatctgca gcgatgtgag
     7021 atacttggcc ggatcgggac cggcgtcctg caacagcact tggacaatca accagggttt
     7081 actaccggcg cgattgacgc cgattttttt gccgcgcagg tcgcggatgt cggcgatttt
     7141 ttccctcgtc acccagacat agggcaccag attatcccag ctgcccagcg cgatcacatc
     7201 cgcgccttgc gcatggctcg gcagcaagcc ggttggattc acgacaaatt cctgcgtaag
     7261 attattgggc agttcaatta atctggccgc gcaaggagat gcatatgagt tggctaccgg
     7321 ctgaagaacc ccagacgaag atggcaccgg cgtgcagggc gctggaacta tcgtggtgcc
     7381 gcggccgcgt gacatcggag gatttgacgt ccgccgtgca ctgccgtctg cggagcggcg
     7441 aaatgtcggc ccgttcgttt tcttcgacca gatgggacct gctgagctgg cgcccggttc
     7501 cgggatcgac gtccgaccgc atccgcacat cggtcttgcc accgtgacct atctgttcga
     7561 gggcacgatc gttcatcggg acagtctcgg cagcgtgcaa gcgatcgagc ccggcgcggt
     7621 taactggatg accgccggac gcggcatcgt acattcagag cgctccgata acgagctcag
     7681 aaaacggcgc cagaaactct ccggcattca gatctgggtc gcgctgccga aacagcacga
     7741 ggagatcgac ccggatttca cccattacgc cgccggctcg ctgccccgaa tagagggcga
     7801 aggcaagacg gtgcgtatca tcgccggctc acttttcggc aaggaatcgc cggtcaagac
     7861 gctctcgcgg ctgttttatg ccgatgccgc tctcgatgcc ggcgcttcac tcctgctcaa
     7921 taacgattat aacgaacgcg ggatttatct tctggagggg gacgtcgaca ttcatggcca
     7981 aagatttgag ccgggccggc tgctggtctt ctcgtccggc gacgagatta ccatcaaggc
     8041 agtagcggcc gcacggttcg tcctgttcgg cggtgaaccg ctcgacagtc cgcgtcatct
     8101 ttggtggaac ttcgtctcaa gctcgcccga gcgcatcgag caggccaagt cggattggaa
     8161 gagcggccgg tttgcgccgg ttcccggtga gtccgagttt attccgctcc ccgagtgaag
     8221 gttggcaatc cgaagccgtg gcggagccga tatcgcgcgc agattggccg cgcgggctgg
     8281 agactgacat tggccgaggc ggggtgacag attggcatcg cgacgtccgg agtgtcgaag
     8341 ttattgaccc ggcgtttgtc aatttaattt aatcaattac gtttcaatat cacggccgac
     8401 taacagctcg cagcatcaag gcgatgcttg atcaatgttt aacatgctca ttgccggtct
     8461 tggttcgcca gtccttgagc taatcgcctt cgggtcgcag agccggaaca ccgtaggccg
     8521 ggaagcggcg tgccgggttt tttcttgcca acatcattgg gtttgtatat tacctacgcc
     8581 aaggagcggc tggtggttaa attatgtttg tgaccaaaca ggcccgcagt ctggccatct
     8641 tttccgcgat tctcctcttc gaaatcatcc tggtttccgg cgcggcgggg cagaagcccg
     8701 gcagatctga agcaaagacg attactctag gaatcgtcgc tgagacaaat caaaaggaga
     8761 tcgaagcgca ttttcaggat tttgtcggct atctggcgaa aaaactttcc tccggatcga
     8821 aggttgaagg aaggatcgtg atcgtttcga cccagtctcg gctcgccagc ctgctttcgg
     8881 cgaggaaggc cgatttctac atggagagcc cccatccgac gcatatggtc aacaacgtat
     8941 acggcgcggg taaattactt ctccggcgct ggaaaggcgg catggccgag taccacgccg
     9001 tgattttcac cgcgaaaaac gacgggacta agcgtctcga agacctgcag ggaaaggtca
     9061 tcgcttttga agatgccgaa tcgacttcag gttattttct cccgaagcta tacctgacga
     9121 aaaaaggctt cagacttgtg cagaagactc aaagcgatgc caaggtctcc agtggcgaga
     9181 tcggttacat ttttgccggt tctcaggaga aactcgtcga cgcggttctc acaaataaag
     9241 tcgccgccgg agcttttagc aacgatgatc acgccgccct tgacgacagc aaaaaattgc
     9301 aaataaccgt tttggcggaa acggcgagtt tgccgcgcca ccttgtctct gttcgaaaag
     9361 atttggcccc ggagctggcc aagggactgg agaaggctct actgtcgatg caccaagatc
     9421 ccgagggacg gtcgatattg caaaaagccg acggaacgac aaagttcgac cccctaccgg
     9481 gcggggagtt ggcggtgcgc cagcggttgc tcgataccta ttactcgccg cagaaaaagt
     9541 agaaaaccct gtcgaatcga tcccaaagcc gatccccgga atgccgcgtc gaccttgccg
     9601 tagaatcgat catcgcgtag tttcccatct gacgtctgcc aaaataaatt tgtcgctgaa
     9661 ttcaccggca gatttcatgg ataatatctc ccgcgggttt tttggcgtcg attgcgacca
     9721 tgcgccgctt tggcagaaag ggtctgcaat gaagtttaag cgccggctgc agcagctttt
     9781 actttcggcg ttgattacgt cggccaccag cgccatggca gcaacgctgt tttccgaagc
     9841 ctggagtgat ctcaccaaat atttcacggc tgctgcgtcg ctggttctcc aggcctcgat
     9901 cggcctcgat aacacgaggt tcgatacttt gccgcccgcc cagcgcctgg aggcgagaac
     9961 acagctgcaa aagttaaaag atcaactgaa tatattattc ctcgagcagt ccacgctggt
    10021 aagtcagttc gagtttttcg tcaaaatcgc tagggaccca tcgaaaacgc ccgcggaaag
    10081 gaaaacatat tgggacaata atgtcctgcc tcggatcagc actgtttccg aagcggtgag
    10141 cgaggtcaga actttttcgg agaaggacgg ccagcccttc agtgtcgtcc tttcggccga
    10201 cgatcgcctc gccctcggtg acacactcgc cgcgcgcggc attgccctga aaactttcga
    10261 gaagatggct cctcccgctt cggcggacga agtgctgcaa ttccaggggc tcatcaacga
    10321 ctataatgtt ttgcggcaga atctattcaa actccgtctc gccatcgatg cggcgttggg
    10381 ccggctcgcg agcgcctgaa cgtggcccag ctattcatca gttacgcgag cgaagatcgc
    10441 aattttgtcg acagcctcgc cggcggcctc actttggcgg gtcatgacgt ctggtttgac
    10501 cgcgatctcg atgccggtcc ctaccgcgag cagattcttg cgcggctgac gcgcgcccag
    10561 gtagcgatcg ttgtttggtc agcgcgctcg aaacagagtc gctatgtcat cgatgaagcg
    10621 gaacgcgcag tgtcgcgcgg catattgctg ccgattcgca cggatgacac tgatctgccg
    10681 ctcggattcg gcgggttgca aactttcgat ctatcttcgt ggtccgggcg agccgacgat
    10741 ttgcagttca cagtgctcct ggatcaaata gacaaaatcc taaaacatcc tacggtctcg
    10801 cgaggacgtc cggctgttcc tttcattgcg aagtcgctgc tgcttgctgg ctttgcagcg
    10861 gcgatttgcg ctcccgctct agcgtggctc tacgccgtaa gaaatggctc tgcttcgacc
    10921 ccgcacctgt acacgatcct gcaagccttc gtcctggctg ccgtttgcac tactccggtc
    10981 atgctctggt gtggcttcga ggtcggccgc ttcggactgt ctcatgccgc tcccatcctg
    11041 cgcagggcca tcggcattta tgcaattgcg gcggtcgtgt cgttagctct tgtcgcggcg
    11101 gcggttgttg gcggcgtgac cacgggacgg acgccggccg ccgcactagg acagattttt
    11161 ttcgtcgctt ttcccgcgac gttgacggta ggcgtagtta tcaccttgct gaaggccacg
    11221 gcttatctga tcaagaaact gcgaatgtga aatggcagcg caggcacagg gtcggcggta
    11281 gagtcgagcc cgagttttga ttcattgcat atggacaggc gcgcacgggc aggctattac
    11341 agcttgatca actaacccga ccaaagagag gcaaattata gatgagattc tccgctgcga
    11401 tctgtttatc cttatcactg ctgtcgattg agttcttgct acccggcgcg gcgccggcgc
    11461 aggcgcccta ctacgcgggg aaaaccat
//
LOCUS       DASQKS010000397         6734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1881652, whole genome shotgun sequence.
ACCESSION   DASQKS010000397 DASQKS010000000
VERSION     DASQKS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881652"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VE170_10315"
     CDS             <1..424
                     /locus_tag="VE170_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ15872.1"
                     /translation="VTHEVNRPYFERAALNSWNLAPDRLAKSKKKPVFQTMGDNMVLT
                     DGTRSVELYQITNNTHHDGIVMAYLRKEKLLIEADAFNPLAAGAEPPKVPNPFWVALE
                     ANVRRLNIDVDRIVPIHGRMVPYSDLLGAIGKKPAPAK"
     gene            543..1574
                     /locus_tag="VE170_10320"
     CDS             543..1574
                     /locus_tag="VE170_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15873.1"
                     /translation="MKNLTAILLFIFLTHSDAGAQSSYFQGKTVRLIVGYQAGDTHDL
                     WARAYARHMGKHLPGNPNFVVQNMPGAGSMVAANHVYNVAKPDGLTLGSIAPGLYLAQ
                     LTGGKEVKFDWARFSWIGTPEHNGTLLFMRADAPFKSIEDIRKAKEGPKCSATGVGTS
                     GHLIPRLLEETLNLKFQIVTGYPGGADQDLALERGEVQCRAITVAAYFGREPFISWYK
                     KGFVRILIQTSRKRHARIPEVPTLWEYMDKEKLPESNRRLAVVALGAGGFGSWPIVST
                     PKLPVELLKTLREAYAKTLQEPDLMDEAKKRGWELRPVGGDDLATLAKEVSAQPADVV
                     ERMKKLMGE"
     gene            1619..2647
                     /locus_tag="VE170_10325"
     CDS             1619..2647
                     /locus_tag="VE170_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15874.1"
                     /translation="MMKFFFSVMVILSAWVNVHAQAPFYQNKTVTIVVGYLAGDGYDI
                     WARLLAAHMGKHIPGNPNFVVNNMPGAGSMIAANNVYNVAKPDGLTMAAIGPSLYLDQ
                     VIGKKEVQFDWGKFGWVGSTENTPWLFYMSTNTPFKTIDDVRKAKEAPKCSATSSGTS
                     GHFIPKLLEEAMGAKFTVVMGYQGGSEQDLAFERGEVVCRALSIPTFFAREPFTTWRK
                     KNMVRILLQTGRKRDAKMPDVPTIHELMTEYKTPASTRALVMAILASGDLGRPFITPP
                     AIPAERLKILREAFRKTMADPGFLADVKARKLEIDPDYGEQLEALAKEIVSQPREISD
                     RIRKLLSE"
     gene            2898..3389
                     /locus_tag="VE170_10330"
     CDS             2898..3389
                     /locus_tag="VE170_10330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ15875.1"
                     /translation="MSQVKCPSCSREFVRRVVRAGLRERLAGWFFIYPFKCQICGVRF
                     YSLQWGVRYLRLREDNRDYDRLAMNFPVLFRGDRFDGAGSVTNISMGGCSFTTASKLT
                     RRAIMRLELKVSDEAAPVIVELAVLRHWHNQVAGVEFLRCERSEHERLQHFVHGLLIG
                     RSS"
     gene            complement(3395..5341)
                     /locus_tag="VE170_10335"
     CDS             complement(3395..5341)
                     /locus_tag="VE170_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HYJ15876.1"
                     /translation="MAEGSIDPSQIAAAQRFVDEEAGPRRHLTGAIGTITSALAVGMS
                     LTFLYWAWASVTAQVLRLVFLGLALTLTFLIYPTHRSADRTTIPWHDWLLALASIAVV
                     AYPLWDFEEFIYRAAMPTEPDQIFGGFTMLLVLEATRRTTGWALPVVAVSFLAYAIFG
                     AYLPSPWTHQGYQLDRLVGHMYITLEGIFGVPLDVAATFIILFTIYGAVLEFSKAGEF
                     YVNFSLAATGGKPAAAGRTVTLASFLLGGPSGSGVATTVTLGSVAWPLLAKAGYDKDS
                     AGGLLSAGGIGAIISPPVLGAAAFLIAEFLKISYLEVLAMAAIPTLLYYWSIFTMVEL
                     DARKFGAKPISVEGESMGSLTRKYGFHFLSLVAIVGFMMWGYTAILSVAYAIVLGIAL
                     SYMRRDTALTPGRIWRLFEKGTVDVLSVASTCATAGIIVGVVTLTGLGLKFSLIILTL
                     AGNSLFLTVLYTALSLWVLGLAVPVTASYIIAAVITAPAMIKLGIPEYAAHMFIFYYA
                     VLSEVSPPTALAPFAAAAITGGSPYKTTMMAWKYTAPAFLVPFMFTLHPDGIGLLLKG
                     EWSNILWTAATAAVGLGALAAGLTGWLIVKTTLAERAILIMAGLVLVYPSLVQDAIGI
                     GLFGLAGVLQYRRHRTPPVGEIIK"
     gene            complement(5334..5978)
                     /locus_tag="VE170_10340"
     CDS             complement(5334..5978)
                     /locus_tag="VE170_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15877.1"
                     /translation="MLCALVAVIAMIDPAGSIQLQDISRRIVTWNSHLLVLTTSGTAE
                     FSTPGVPAIVQDRFPAIPLFQTRQTQPQFSTSLRSTAQSMCIELSSDTGAPRSLPIDV
                     GSMLRLSFRHSIYGSRVEELFALRRDGFLLTRLRYGEARLVDFYGYENAKLDNGAWVV
                     NPPPVLLPSISLNTSADGAMSLQLDRHDHTKPHAIQTTGALRLTLASCRNNPHG"
     gene            complement(6023..>6734)
                     /locus_tag="VE170_10345"
     CDS             complement(6023..>6734)
                     /locus_tag="VE170_10345"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ15878.1"
                     /translation="AWDGFSGVIKGLPAKANIRALMALYSNFMHIVAADGSGIKSVAD
                     LKGKRVSTGAPGSGVEVKGLRVLEAYGLTLKDLKSQERLGAGESAGALKDRKIDAFFW
                     DGGLPTGAVLDLAATPGIKIHLIPHGDAIPKMTAKYGPLYFTANIPKGMYKGIDEDTP
                     VAAATNLLIANEKLDENLAYQITKLLLEHTADLVAVHKAATEMSLKNAVVGSPIPFHP
                     GALRYYKEKGIKVPTPGK"
BASE COUNT         1447 a         1873 c         2063 g         1351 t
ORIGIN      
        1 cgtcacccac gaagtgaacc gaccttactt tgagcgcgcg gcactgaact cgtggaatct
       61 cgccccggac cgtttggcaa agtcgaagaa aaagccggtg tttcaaacca tgggcgacaa
      121 catggttttg accgacggca cgcgctcggt tgagctctac cagattacca acaacaccca
      181 tcatgacggc atcgtcatgg cgtatctgcg caaggaaaaa ctcttgatcg aggctgacgc
      241 cttcaatcct ctcgccgcgg gagcggagcc gcccaaggtg cccaatccgt tctgggtagc
      301 cctcgaggcc aacgtacgac gcttgaatat agacgttgat cgcattgtgc cgatccatgg
      361 ccggatggtt ccttatagcg acttgctcgg cgccatcggc aagaagccgg caccggcaaa
      421 gtaatcggcc aactgacatt ggagtcgtgg tgctatgaag tttttgagtg gtgggtttgt
      481 caaaccccaa cacttgatta ttccatcgat ccacgactcc ctcgccgctt tttctaactt
      541 cgatgaaaaa cctcactgct atcctgttat tcattttcct gacgcactcc gacgccggcg
      601 cgcagagttc ctattttcaa ggcaagaccg tgcgcctgat cgtcggctac caagccggcg
      661 acactcatga tttatgggcg cgcgcttatg cgcgccatat gggcaagcac cttccaggca
      721 atccgaactt tgtcgtgcaa aatatgcctg gggcaggctc catggtggcg gccaatcacg
      781 tttacaatgt cgccaagccc gatgggttga cgctcggttc gatcgccccg ggtctttatt
      841 tggcgcagct caccggcggc aaggaagtca aattcgattg ggccaggttt tcctggatcg
      901 gcactccgga gcataacggt acgttattat tcatgcgcgc cgacgcgccg ttcaaatcca
      961 tcgaggacat tcgcaaggcc aaggaggggc ccaaatgttc ggcgactgga gtgggcacga
     1021 gcggccattt gattccgcgc ctgctcgaag agacgctgaa tttaaaattt caaatcgtca
     1081 ccggttatcc cggcggcgcc gatcaggact tggctctcga acgcggcgaa gtgcagtgcc
     1141 gggcgatcac ggtggcggct tatttcggcc gcgagccgtt tataagctgg tataaaaagg
     1201 gatttgtgcg gatcttgatt cagacctcgc gcaagcgcca cgcgcggatt cccgaggtgc
     1261 cgacgctgtg ggagtatatg gacaaagaga aattgcccga gagcaatcgg cgcctggcgg
     1321 tggtcgctct cggtgccggt ggcttcggct cctggccgat cgtgtcgacg cccaaattgc
     1381 cggtggagct attgaaaact ttgcgcgaag cctatgcgaa aacattacag gagccggatc
     1441 tcatggacga ggcgaagaag cgcggttggg aactgcgtcc ggtcggtggc gacgatctgg
     1501 cgacgctggc caaggaagtg agcgcgcaac cggccgacgt ggtcgagcgg atgaagaagc
     1561 tgatgggcga atgagacgga gaagaaacct gagttcgtat gattgggagc agtaaattat
     1621 gatgaaattt tttttcagcg tgatggtgat tctaagtgcg tgggtcaatg tccacgcgca
     1681 ggcgccgttc tatcagaaca aaacggtgac catcgtcgtc ggctatctgg caggcgacgg
     1741 ctatgatatc tgggcgcggc ttttggcggc gcatatgggc aagcatattc ccggcaatcc
     1801 taacttcgtg gttaataaca tgcccggcgc cgggtcgatg atcgcggcga acaatgttta
     1861 caatgttgcc aaaccggacg ggctaacgat ggcggccatc gggccgtcgt tgtatctcga
     1921 ccaggtaatc ggcaagaaag aagtgcaatt cgattgggga aaattcggct gggtcggctc
     1981 gacggagaat actccgtggc ttttctacat gagcaccaat acgccgttca aaaccatcga
     2041 cgatgtgcgc aaagccaagg aagcgcccaa gtgttcggcc accagcagcg gcaccagcgg
     2101 ccattttatt cccaagctcc tcgaagaggc gatgggcgca aaattcaccg tcgtcatggg
     2161 ctatcaaggc ggctctgagc aggatctggc cttcgagcgc ggtgaagtgg tctgccgggc
     2221 gctgagcatc ccgacttttt tcgcgcggga gccttttacc acgtggcgca agaaaaatat
     2281 ggtgcgtatc ctgttgcaga ccggccgcaa gcgcgatgcc aagatgcccg atgtgccgac
     2341 gatccatgag ttgatgaccg aatacaagac gccggcttcc actcgtgctc tggtgatggc
     2401 gatcctcgcc tccggcgatc tcggccggcc gtttatcacg ccgccggcga ttcccgctga
     2461 acgtctaaag atcctgcgcg aggcctttcg caaaaccatg gccgatccag ggttcctggc
     2521 cgatgtcaaa gcgcgcaagt tggagatcga tcccgactat ggcgaacagt tggaagcgct
     2581 ggccaaagag atcgtctctc agccgcgcga gatttccgat cgcataagga aactgctatc
     2641 cgagtagtcc gcgaaggttg cgtccgtgcc gaccaatcta cccggcccta ggggcggcgg
     2701 gcgacgttga gacaattgtc ctgcggtttg gcacttttat ccgcatggcc atcccgttgc
     2761 taattgcctt tcgccggtcg ccatttgcca atcgttgcgc aagcgccgct caagaaattg
     2821 ccggtgatgt ttggccggaa ctctcaatgt gttagctatg ggagaaggat gccatctaat
     2881 gcgtggagag ttggcttatg tcgcaagtga agtgtccgag ttgttcccgc gagtttgtcc
     2941 ggcgtgtcgt cagagccgga ttgcgggaaa ggctagcggg ctggtttttc atttatccct
     3001 ttaaatgcca gatctgcggc gtgcgttttt attcgctcca gtggggcgtg cgctatctaa
     3061 ggctgcgcga ggacaatcgc gactacgacc gtctggcgat gaacttcccg gtgctgtttc
     3121 gcggcgatcg gttcgacggc gccggcagcg tgacgaatat ttccatgggc ggctgtagtt
     3181 ttaccacggc gagtaagctt acccggcgtg cgattatgcg gttggaactc aaggtgtccg
     3241 acgaggccgc gccggtgatc gttgaactcg cggtgctgcg ccattggcac aatcaagtcg
     3301 ccggcgtcga gtttcttcgc tgcgagcgaa gcgagcatga gcgcttgcag cacttcgttc
     3361 acggcctgct gatcgggcga agctcgtaga ccgtctattt tataatttct ccaactggcg
     3421 gcgttctgtg ccgccggtac tgtaaaaccc cagccaggcc aaataagccg atgccgatcg
     3481 catcttgtac gaggctcgga tagaccaaca ccaatccggc catgatcagg atggcccgct
     3541 ccgctagtgt ggttttgacg atcaaccagc cggtcagacc ggcggcgagg gcgccgaggc
     3601 cgaccgccgc ggtggcggcg gtccacaaga tattgctcca ttcgcccttc aacagtaagc
     3661 cgatgccgtc gggatgaagc gtgaacatga acggtacgag gaacgccggc gcagtgtatt
     3721 tccacgccat catcgtggtc ttgtacgggc tgccgccggt gatcgcggcg gcggcaaagg
     3781 gcgccaacgc ggtgggcggc gagacctccg agagcacggc gtagtagaaa ataaacatat
     3841 gcgcggcgta ctcgggaatc cccaacttga tcatcgccgg cgcggtgatg acggcggcga
     3901 tgatatacga cgccgtcacc ggcacggcga gaccgagcac ccagagagac aacgcggtgt
     3961 agaggacagt gagaaacaga ctattgccgg cgagggtgag gatgatcagc gagaacttta
     4021 gcccgaggcc ggtgagcgtg acgacgccga ctatgatgcc ggcggtggcg caggtcgatg
     4081 ccaccgacaa aacatcgacg gtgccctttt cgaacaaccg ccagattctt cccggcgtga
     4141 gcgcggtatc gcgccgcatg tagctcagcg cgatgccgag gacgatggca taggcgaccg
     4201 aaagtatcgc ggtatagccc cacatcatga aaccgacaat ggcgacgagc gagaggaagt
     4261 gaaagccgta cttgcgggtc agactcccca tgctctcgcc ttcgactgag atcggcttgg
     4321 cgccgaactt tcgagcgtca agttcgacca tggtgaagat cgaccagtaa taaagcagtg
     4381 tcgggatcgc cgccatggcg agcacttcca ggtatgaaat cttgagaaac tcggcgatga
     4441 gaaacgccgc ggcgcccaac accggcggcg agataatcgc gccaatgccg ccggccgaca
     4501 ataggccgcc tgccgagtct ttgtcatagc cggctttggc gagcagcggc cacgcgaccg
     4561 agcccagagt cacggtggtt gcgacgccgg aaccggaagg gccaccgagc aggaacgatg
     4621 cgagcgtgac ggtgcgcccg gcggcggcgg gtttgccgcc ggtggccgct aaagaaaaat
     4681 tgacgtaaaa ctcgccggct ttggaaaatt ccaacaccgc gccatagatc gtaaacaaga
     4741 tgataaacgt cgcggcgacg tcgaggggca cgccgaagat gccttcgagg gtgatgtaca
     4801 tgtgaccgac caagcggtca agctgataac cttgatgggt ccagggcgac ggcaaatagg
     4861 cgccgaagat ggcgtaggcg agaaaactga cggcgaccac aggcagtgcc caacccgtag
     4921 tgcgccgggt cgcttccaaa accaagagca tggtgaagcc gccaaaaatc tgatccggct
     4981 cggtcggcat cgcggcgcgg taaatgaact cttcgaaatc ccacagcggg taggcgacca
     5041 cggcgatact ggcaagcgcg aggagccagt catgccaggg aatagtggtt ctatcggcgc
     5101 tgcgatgggt cggatagatc agaaaagtaa gcgtcagcgc aaggccgaga aaaaccagcc
     5161 gcagcacctg cgcggtgacg ctcgcccatg cccagtaaag gaacgtcagc gacatgccga
     5221 cggcgagcgc cgaagtgatt gtgccgatgg cgccggtcaa atggcgccgc ggtccagcct
     5281 cctcgtcaac gaatcgctgc gccgccgcga tctgcgacgg gtcgatggag ccttcagcca
     5341 tgggggttgt ttctacagga tgcaagagtc aatcgcagcg cgccggtggt ttgaatcgcg
     5401 tgcggtttgg tgtggtcatg gcgatcgagc tgaagcgaca tggcgccgtc ggcgctagtg
     5461 ttaaggctga ttgacggcag cagcaccggc ggcgggttga ctacccacgc gccgttgtca
     5521 agtttggcat tttcatagcc gtagaagtcc accaagcgtg cttcaccgta gcgtagtcgc
     5581 gtcaataaaa acccgtcgcg ccgcagcgca aacaattctt cgactcggct gccatagatc
     5641 gagtggcgga aactcaagcg taacatgctg ccgacatcta tgggcaggct gcggggcgcg
     5701 cctgtgtcgc tcgatagctc aatgcacatc gactgcgccg tgcttcgcag cgacgttgag
     5761 aattgcggct gcgtctgccg cgtttgaaaa agcgggatcg caggaaatct gtcttggacg
     5821 atggccggca cgcctggtgt cgaaaactcg gccgtgcctg acgttgtcaa aacaaggaga
     5881 tgggagttcc acgtgacgat ccggcgggaa atatcttgca gctggatact gcccgccgga
     5941 tcaatcatcg cgatcacggc gaccaaagcg cagagcatga ggcagcagac caaaccgcct
     6001 cgcctgaacc tcgccggtgc aatcatttgc caggtgtcgg caccttgatg cccttttctt
     6061 tgtagtaacg cagcgcgccc ggatgaaacg gaatcggcga gccaaccacg gcatttttca
     6121 agctcatttc tgttgctgct ttatgcacgg cgaccagatc ggcggtgtgt tcgagcagca
     6181 gtttggtgat ttggtaggcg agattctcgt cgagtttttc attggcgatc aacagattag
     6241 tcgccgcggc gaccggcgtg tcttcatcga tgcctttgta catgcccttg ggaatgttgg
     6301 cggtgaagta aagcgggccg tacttcgcgg tcatcttagg aatcgcatcg ccatgaggaa
     6361 tcaagtggat tttgatccca ggtgtcgctg ctaaatccag cactgcgccg gtgggcaggc
     6421 cgccgtccca aaaaaacgca tcgatcttac ggtctttgag agcgccggcg gactcaccgg
     6481 cgccgagccg ttcctggctc ttcaggtctt tcagggtgag gccgtacgct tccagcacgc
     6541 gcagcccttt gacttcgaca ccggagccgg gcgcgccggt ggacacgcgc ttgcctttga
     6601 gatcggctac cgacttgatg ccgctcccat cagcggcgac gatatgcata aagtttgagt
     6661 agagcgccat caaagcccga atattcgcct ttgccggcag tcccttgata acgccgctga
     6721 agccgtccca ggcg
//
LOCUS       DASQKS010000398         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2328102, whole genome shotgun sequence.
ACCESSION   DASQKS010000398 DASQKS010000000
VERSION     DASQKS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328102"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VE170_10350"
     CDS             complement(<1..307)
                     /locus_tag="VE170_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ15879.1"
                     /translation="MQAEKAQLVIQGLKEAGIDFASGVPDAQFIEVYKMLAEDRDIRY
                     VGAANEAEATGIGFGAWFGGKKPALILATSGLLVATYHLARINLLHEVPVLIVIPYRG
                     "
     gene            complement(321..1379)
                     /locus_tag="VE170_10355"
     CDS             complement(321..1379)
                     /locus_tag="VE170_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15880.1"
                     /translation="MKAIRRKVELAVVSVLWLGSVAAVAFAQAPFYQGKTITIIRGSA
                     PGGVGEMRTRAVANYLKKHVPGNPTVVIEFMAGAGGTKAANHLYRGARADGLIIGSAP
                     SGMVSSALLGEAGVQYDLDKFIYLGSPNSESHYVFFTNRKLGLGNLEKLRAHSGLRIG
                     AQTVGHPIYLTGRLFAYLLGLKDPKYVVGYSSPELDVAMLSGELDAGSGVASSIVKQN
                     PEFLDKGLVDFHSIIEIPRGDKHPRFAQLPELDTSVKSDKERKLLALHRTFRLAGSPF
                     ILPPGTPKDRAEVLREAMRKIYADREFLAEYLKLTGEEASPLLPDANDKAIRELPRDP
                     EVIEFYKLFGGTQALPPR"
     gene            complement(1354..3609)
                     /locus_tag="VE170_10360"
     CDS             complement(1354..3609)
                     /locus_tag="VE170_10360"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ15881.1"
                     /translation="MSTDKRSFSVVGNSVPRVDGADKVTGRAKYVADLIIPGMIEGVF
                     LRSPYAHARIVSIDAREAEALPGVFAVLTSKDFSDISPYMGRGNRKDQPIIAVDRVIY
                     AGQPVAAVAAIDKATAEEALRKIHVEYAELPAVISVEEAMADGAPRLHAFAEKNVCAQ
                     HELVKGDVAKGFAEADVIVEDEFDFPMIYHYSMEPHTSIAQVDSDGITIWTSTGHPFG
                     VRADVAEVFNAPLSKVRVHVNYVGGAYGSKSGGKIEPLVTALARKAGRPVRVVTSVAE
                     AMVTCRRHSIKCLVKTGVKKDGTLVAKQADIFLNTGAYAETGPTVAGRTLTRILGPYR
                     YPNLKITAYCVYTNTVSAASFRSIGGPQTAWATESQMDIIADKLGIDPVELRRKNLVM
                     KGEELRPGYRALDTDLAKGFKLVTDKLGWDGPRSKAGHGRGCGFGTTDPGAPLASTST
                     VHVLADGSVVFMCGTSELGQGAKTIMSQIVAEELRVPLERVTIRSIDTAFTPFDRSTG
                     SSRSTTVMGKAVELAGGDARRQIVELAVEHFECPEEAITLKDGEAIAGGKKVTYGALI
                     NKHFADQGGELVGTGYAHSGMAPTPANPLFWEIGIGAVEVSVDQETGTIHVEKYVTAA
                     DAGKAIHPLQCEGQDEGSAMMGFGHTFYEAYQWEGGQIINSSLVDYKVPTFDDVPDVF
                     ESILIEDGNGPGPYGAKGLGEGGIIPVAPAVANAVFWSTGARVKSLPLTPEKVWRAIK
                     EVANEGDKTKS"
BASE COUNT          736 a         1205 c         1110 g          794 t
ORIGIN      
        1 cgccgcggta tgggatcacg atcagcaccg gaacttcgtg gagcagattg atgcgcgcca
       61 aatggtaggt tgcgaccagt agccctgaag tcgctagaat cagcgccggc ttcttgccgc
      121 caaaccaggc gccgaaaccg ataccggtcg cctcggcttc gttggccgcg ccgacatagc
      181 gaatatcgcg gtcttcggca agcattttgt aaacttcgat gaactgagca tcaggaacgc
      241 ccgaggcaaa atcgatgccg gcttccttga gtccttgaat caccagttgg gctttttcgg
      301 cttgcatatt aaattctccc tcaccgcggc ggcagcgctt gcgtgccgcc aaatagtttg
      361 tagaactcga tcacttccgg gtcgcgcggc agctcccgga tcgccttgtc gttggcgtcg
      421 ggcaatagcg gcgacgcctc ttcgccggtc aacttcagat actcggcgag aaattcccga
      481 tcggcgtaaa tttttcgcat ggcttcgcgc aaaacttccg cgcggtcttt gggcgtaccg
      541 ggaggcaaaa tgaacggcga gccggcgagt ctgaatgtgc gatggagggc gagcaacttg
      601 cgctctttgt ccgacttgac ggaggtatcg agttccggca gctgggcgaa gcgcggatgt
      661 ttgtcgcctc ggggaatttc aatgatgcta tgaaagtcca ccaacccttt gtcgagaaac
      721 tccggatttt gcttgacgat actactggcc acgcccgacc cggcgtcgag ctcgccgctc
      781 agcatcgcca catccagctc cggcgaagaa taaccgacga catatttcgg gtccttcaag
      841 ccgaggagat aagcaaacaa acggccggtg agataaatcg gatggccaac ggtctgagct
      901 ccgatcctca atcccgagtg agcgcgcagt ttttccaaat tgccgagacc gagctttcga
      961 ttggtaaaaa acacataatg ggattcgctg ttcggcgagc cgagataaat gaacttgtcg
     1021 agatcgtatt gcacgccggc ttcgcccaaa agcgccgaag acaccatgcc gctcggcgcg
     1081 ctgccgataa taagaccatc ggcgcgcgcg ccgcgataaa gatgattggc ggccttggtg
     1141 ccaccggcgc cggccatgaa ctcgatcacc accgtgggat ttcccgggac atgttttttg
     1201 agatagttcg ccaccgcccg ggtgcgcatc tcaccgacgc cgcccggcgc gctgccgcga
     1261 atgatcgtga tggttttccc ctgataaaag ggagcttgcg cgaacgccac cgcggccaca
     1321 gaaccgagcc agagaacgct tacaacggca agttcaactt ttcgtcttat cgccttcatt
     1381 agccacctct ttgatcgcgc gccagacttt ttccggcgtt aacggcaagc tcttcacccg
     1441 ggcgccggtg ctccaaaaga ccgcattagc caccgccggc gcgaccggaa tgatgccgcc
     1501 ttcgcccagt cccttggcgc cgtacggtcc cggtccgttg ccgtcttcga tgagaatcga
     1561 ttcgaagacg tccggcacgt cgtcgaaggt cggcaccttg tagtcgacca gggatgaatt
     1621 gatgatctgc ccgccctccc attggtaggc ttcgtaaaac gtatggccga agcccatcat
     1681 cgccgagcct tcgtcctgcc cttcgcattg cagcgggtgg atcgccttgc cggcgtcggc
     1741 ggcggtgaca tatttttcga cgtgaattgt gccggtctct tggtcgacgc tcacttcgac
     1801 cgcgccgatg ccgatctccc agaacagcgg attggccggc gtcggcgcca tgccgctgtg
     1861 ggcatagccg gtgccgacca gctcgccgcc ctggtcggcg aagtgcttgt tgatgagcgc
     1921 gccgtaggtt actttcttgc cgccggcgat cgcttcgccg tctttcagtg taatcgcctc
     1981 ttccgggcac tcgaaatgct ccacagcaag ttcgacaatc tgccggcgcg catcgccgcc
     2041 ggccagctct accgccttgc ccatcacggt cgtcgaccgg ctcgaaccgg tggagcggtc
     2101 gaagggcgtg aatgccgtgt cgatcgatct tatcgtcacg cgctcgaggg gcacgcgcag
     2161 ctcctcggcg acgatctggc tcatgatcgt cttggccccc tggcccaact ccgaagtgcc
     2221 gcacataaac accaccgaac catcggccag cacatgcacg gtcgaagtcg acgccagcgg
     2281 cgcgccggga tcggtggtgc cgaaaccgca gccgcggccg tggcctgctt tcgagcgcgg
     2341 cccgtcccag cccaatttat cggtgaccag cttgaagccc ttggccaagt cggtatcgag
     2401 cgctcggtat ccgggccgca gctcttcacc tttcatcacc agatttttgc gccgcagctc
     2461 caccggatcg atgccgagct tgtcggcgat gatatccatc tgcgattcgg tcgcccaggc
     2521 ggtctgcggc ccgccgatgg aacggaacga cgcagcggaa accgtattgg tataaacgca
     2581 gtaggcagtg attttcagat tgggatagcg gtacgggccg agaatccgtg tcagcgttcg
     2641 ccccgcgact gtcgggccgg tttcggcgta ggcgccggtg ttgagaaaga tatccgcttg
     2701 cttcgccacc agggtgccgt cttttttgac gccggttttg accaagcatt tgatcgaatg
     2761 gcgccggcag gtgaccatcg cttcggcgac ggaggtcacc acccgcaccg gccggcccgc
     2821 cttgcgcgcc agcgcggtga ccaggggctc gatcttgccg cccgacttgc tgccgtaggc
     2881 gccgccgacg tagttcacgt gcacgcgcac tttcgataac ggcgcgttga acacctcggc
     2941 gacatcggcg cgcacgccga aaggatggcc ggtggaagtc cagatggtga tgccgtcgct
     3001 gtcgacctgg gcgatgctcg tgtgcggttc catcgagtag tgatagatca tcggaaagtc
     3061 gaactcgtcc tcgacgatca cgtccgcctc ggcaaaacct ttggcgacat cgcctttgac
     3121 cagctcgtgc tgggcgcaaa cgtttttctc ggcgaatgca tgcagccgcg gcgcaccgtc
     3181 ggccattgcc tcttccacgc tgatcaccgc cggcaattcg gcatactcga cgtgaatctt
     3241 gcgcaacgcc tcttctgccg tcgccttgtc gatggccgcc accgccgcca ccggttggcc
     3301 ggcgtagatt acccggtcca cggcgatgat cggttgatcc ttcctattgc cgcgccccat
     3361 gtacgggctg atatcgctga aatctttgct ggtaagaact gcgaagacgc cgggcagcgc
     3421 ctccgcctcc cgggcatcga tcgagacaat ccgcgcatga gcgtaggggc tgcgcaggaa
     3481 cacaccctcg atcatgcctg gaatgatcag atcggcgaca tacttcgccc ggcccgtgac
     3541 cttatcggcg ccatcgaccc gcggcaccga gttgccgacc acggaaaagc tgcgtttgtc
     3601 agtgctcacc caacacctct tgccaaataa aaactccgct tcagcagatc gaaaccattg
     3661 aaaagcctgc gaactgcgta taacggccct gctgcttctg tcaaggctga ttcagacttg
     3721 tatgcgcgcg gcaacgatgc aatcctttat ggcgacatgt tgcgaccgac gagacgacgt
     3781 tcgacgataa tcgcgttggc aattctttgt tcattgctat cgggtaaaag cgccgtcatc
     3841 ataga
//
LOCUS       DASQKS010000399         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3155615, whole genome shotgun sequence.
ACCESSION   DASQKS010000399 DASQKS010000000
VERSION     DASQKS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3155615"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VE170_10365"
     CDS             complement(<1..587)
                     /locus_tag="VE170_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ15882.1"
                     /translation="MRLSGRVAIVTGGARHIGAAYCRRLAEEGAAVVIADILDGENIA
                     NEIRSKGGKAFALKIDVSNEEDTSRMASEAVKEFGRIDILVNNAAIFINIQRHPFYEI
                     TSDEWDKVSAVNIKGPFLCAKAVFPQMKEQKSGKIINISSSTAYWGTPNFLHYVASKA
                     ALIGMTRSLAREVGEFGICVNAIAPGLVEHEGQNAP"
     gene            693..1670
                     /locus_tag="VE170_10370"
     CDS             693..1670
                     /locus_tag="VE170_10370"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15883.1"
                     /translation="MLVGFAASLLIVGTADAQALKKIKIGYPSLSFRQSNVWVAREMG
                     LFAKYGLEVEPILFRGGQVATQALVSGDPPIVNIGTVVQATIQGHNLVLTAAVETKYD
                     LIVFSRPGITQLEQLKGKRLGITGFNSATHYASIILARHLGVELKEFTLIPAGLDTER
                     IASVMSGVIDATYLSSSAAPLGRRAGLVEHVSIGDLGVEVQGNGFATSRAYIASNRET
                     VRSALRGFVEAIYFVYGNKAATQKVFAKYMRTNDPAVLEDSYNGYIKSIPKKPYPTLK
                     GIQFMLDVLTPTLPNAKNFKPEQFVDLSFLQEMEKDGFFNEMAKRYPSK"
     gene            1776..2165
                     /locus_tag="VE170_10375"
     CDS             1776..2165
                     /locus_tag="VE170_10375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ15884.1"
                     /translation="MARKDVQPKTLSDPRPRYTQAIQTKGGKLLFIAGQTASDKDGNV
                     VGKGNIKKQTEQVYANINAVLKKAGGSIDNLVMTTTYIVDRKYRPGYNEVRMKQYKKK
                     SPTSTLVIVKGLADPDYLIEIAGIAVI"
     gene            2247..3236
                     /locus_tag="VE170_10380"
     CDS             2247..3236
                     /locus_tag="VE170_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HYJ15885.1"
                     /translation="MKVTVLDDYQHAIAPTPAIARLRQSVDVQIIHEKLADEAAIVEA
                     LKDSQAVMPIRERTKFTAALFHKLPALEYISQTGNHAYHIDMEAATAHGVVVSLAPGG
                     NSTTELTFGLMLDVMRHISQGDQALRRGEWPLMLGYVLKGKTLGILGLGKIGTEVAAI
                     ARAFGMNVIAWGPTLNQARAAKSSATFMPLEDVLRGADVVTVHLKLSEESKNLLNETR
                     LRLMKKTAYLVNTARGAIVDETALVKILRAKTIAGAALDVFVEEPLAKNHPILELDNV
                     VLTPHLGWPTDSGFEGFAENAVTNIFDYMAGKLTRALNPEALNHRRKRNQSTP"
     gene            complement(3359..>3910)
                     /locus_tag="VE170_10385"
     CDS             complement(3359..>3910)
                     /locus_tag="VE170_10385"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYJ15886.1"
                     /translation="RTAADPEQIYRAVRWGRAVDLLILDTRQYRAADRSTMLGRRQKD
                     WFFDRLVNSSATFKFVATTVPMAGGGQDRWDGYPQERDEILRFIHRQKIPGVVFMSAD
                     MHYAAVTKIPRSNGLIDITAGPLAAQLNRVTNSANSRYEFFLAENFNFAKITVDPKVN
                     ANEALLEFIDQDNRVFFTRKIRA"
BASE COUNT          892 a         1180 c         1052 g          786 t
ORIGIN      
        1 ggcgcgttct gaccttcatg ctcgaccaaa cccggagcga tggcattgac gcaaatgccg
       61 aactcgccga cttcgcgcgc caacgaccgg gtcatgccga tcagcgccgc tttcgacgcg
      121 acatagtgta aaaagttcgg cgtgccccag tacgccgtcg acgacgagat gttgataatc
      181 ttgccgctct tctgctcttt catctgcgga aagaccgcct tggcgcaaag aaacggccct
      241 ttgatgttta ccgccgacac tttgtcccac tcgtcactgg tgatttcgta aaacggatgg
      301 cgctggatgt tgatgaagat ggcggcgttg ttgaccagaa tatcgatgcg gccgaattct
      361 ttcacggctt cgcttgccat gcggctggtg tcttcttcgt tcgacacgtc aattttgagc
      421 gcgaacgcct tgccgccctt cgaacgaatc tcgtttgcga tattttcacc gtcaagaata
      481 tcggcgatga cgaccgcggc gccttcttcc gccaagcgcc ggcagtatgc cgcgccgatg
      541 tggcgtgcgc cgccggtgac gatggcaacg cggccgctca gcctcaccgg ccactcccag
      601 ggaaatggag tgttggagta ctggagtatt gggtttcgaa cccatcactc caccactcca
      661 ttactccatc actccaggtc gcccgcgctt tgatgctcgt tggcttcgcc gccagtctgc
      721 tcatcgtcgg caccgctgac gcccaagcac tcaagaagat caagatcggc tatccgtctt
      781 tgagctttcg ccagagcaat gtctgggtcg cgcgcgagat gggcttgttc gccaagtacg
      841 gtttggaagt cgagccgatc ttgtttcgcg gcggccaggt ggcgacccag gcgttggtca
      901 gcggcgatcc gccgatcgtg aatatcggca ccgtcgtcca ggcgaccatc cagggtcaca
      961 atctcgtgct caccgccgcg gtcgaaacca aatacgacct gatcgtgttt tcccgtccgg
     1021 gaatcaccca gctggaacaa ctcaaaggca aacgcctcgg catcaccgga ttcaactcgg
     1081 caacccatta cgcttcgatc atcctcgcgc gccacctcgg cgtcgaactc aaagaattca
     1141 ccctgatacc cgccggtctc gacaccgagc gcatcgcctc ggtgatgtcg ggtgtgatcg
     1201 atgccaccta cctgtcatcc tcggcggccc cgctgggacg ccgcgccggt ctggtcgagc
     1261 atgtttccat cggcgatctc ggcgtcgagg tgcaaggcaa cggcttcgcc acctcgcgcg
     1321 cctatatcgc cagcaatcgc gagacggtgc ggagcgcgct gcgcggcttc gtcgaggcga
     1381 tctactttgt ctatggcaac aaagccgcga cgcaaaaggt cttcgccaaa tacatgcgca
     1441 ccaacgatcc ggcggtgctt gaggattcct acaatggcta tatcaaatcg ataccgaaga
     1501 aaccttatcc aaccctgaag ggcattcaat tcatgctcga cgtcctgacg ccgaccctgc
     1561 ccaatgctaa gaatttcaag cctgaacagt tcgtcgatct ttcttttctc caagagatgg
     1621 agaaagatgg tttctttaac gaaatggcca agcgataccc gtcgaagtag acaagcacgg
     1681 agcaattgga gtaatggagt ggtgagtggt ggcttggatc aacaacccaa caatccaata
     1741 atccattact caaatttata aaccgaggag tttccatggc acgcaaagac gttcaaccga
     1801 agacgctcag cgatcccagg ccgcgttaca cccaggcgat ccaaaccaag ggcggcaagc
     1861 ttctattcat cgccggacag accgcatcgg ataaggacgg caacgtggtc ggcaagggta
     1921 atatcaagaa gcagaccgaa caggtctacg ccaatatcaa cgcggtgctc aagaaagccg
     1981 gcggctccat cgataatttg gtcatgacga ccacctacat cgtcgaccgc aagtatcgcc
     2041 ccggctacaa cgaagtacgc atgaagcagt ataagaaaaa atccccaacc agcaccttgg
     2101 tgattgtcaa aggcctagcc gatcccgact acttgatcga aatcgccggc atcgcggtaa
     2161 tctgaaagaa agaaatttca ccgcaaaagc gcagaggacg cgaaaatgta aattgacctc
     2221 cgcgagtctg cggtgagact gccgaaatga aagtcaccgt tctcgacgac taccaacacg
     2281 ccatcgcgcc gaccccagcc atcgcccgac tgcggcaaag tgtcgacgtg cagatcatcc
     2341 atgaaaagct cgccgacgag gcggcgattg tcgaagcgct caaggactcc caagcggtga
     2401 tgccgatccg cgagcgcacc aaattcaccg cagcgttatt tcataagtta ccggcgttgg
     2461 aatacatctc gcagaccggc aatcatgcct accacatcga catggaggcg gcgacggccc
     2521 acggcgtcgt cgtcagcctc gctcccgggg gcaacagcac caccgaactt acttttggct
     2581 tgatgctcga tgtcatgcgg cacatttccc agggtgatca ggcgctgcgc cgcggcgagt
     2641 ggccgttgat gctgggctat gttttgaaag gcaaaaccct gggaattttg ggcctcggca
     2701 agatcggcac ggaagtagca gctattgccc gagccttcgg catgaacgtg attgcctggg
     2761 gaccgacctt gaaccaagca cgcgccgcga aatcgtcagc cacatttatg ccgcttgaag
     2821 atgtcctgcg cggcgccgac gtcgtcaccg tccacttgaa gctatccgag gaaagcaaga
     2881 acctcttgaa cgaaacccgc ctgcgcttga tgaagaagac ggcctacctg gtcaacacgg
     2941 cgcgcggcgc gatcgtcgac gagacggcac tggtgaaaat attgcgggcg aaaaccatcg
     3001 ccggcgccgc tcttgatgtc ttcgtcgaag aaccgctggc gaaaaatcat ccgatcctgg
     3061 aactcgacaa tgtcgtcctg acgccgcacc tgggctggcc caccgactcc ggcttcgagg
     3121 gcttcgccga aaatgccgtg actaacatct tcgactacat ggcaggcaaa ctcacccgtg
     3181 cgctcaatcc cgaggcgctg aaccatcgcc gcaagcgcaa tcaatccacg ccgtagttgc
     3241 aaaatctgcc gacgagaatt cgactgacag ccatcattcc gtctctgggg aaatccacgc
     3301 gattgttgct gcaacaacgg cggcaatggc tgaccgtctg caccaatgca gttttcactc
     3361 aggcgcggat cttccgcgtg aaaaacaccc gattatcttg atcgatgaac tcaagcagcg
     3421 cctcgttggc attcaccttg gggtcgacgg taattttggc gaagttgaaa ttttccgcga
     3481 gaaagaattc ataacggcta ttggcgctgt tggtgacgcg gttgagctgc gcggccaaag
     3541 gcccggcggt gatgtcgatg agaccattgc ttctgggaat cttggtcacc gccgcgtaat
     3601 gcatatcggc gctcatgaat accactccgg gaattttctg acggtgaata aagcgcaata
     3661 tctcgtcccg ttcctgggga taaccatccc agcgatcctg accgccgccg gccatgggca
     3721 cggtggtcgc gacgaactta aaggtcgcgc tggagttgac caaccggtcg aaaaaccagt
     3781 ctttttgccg gcgtcctaac atggtgctgc gatccgccgc gcggtattgc cgggtatcga
     3841 gaataagcaa atccaccgcc cggccccagc gcaccgcgcg ataaatctgc tccgggtcag
     3901 ccgccgtgcg
//
LOCUS       DASQKS010000400         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3579319, whole genome shotgun sequence.
ACCESSION   DASQKS010000400 DASQKS010000000
VERSION     DASQKS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3579319"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /gene="rnr"
                     /locus_tag="VE170_10390"
     CDS             complement(<1..1706)
                     /gene="rnr"
                     /locus_tag="VE170_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HYJ15887.1"
                     /translation="MIDKTQEKILGLFNKQPRARFTPAEVQRRAGIERGDLQAIIDAL
                     RQLCRDGRLVRLKKNHYALPDRQNLLRGRVHAHPDGYGFLILEDKGSDDVYLNRREMR
                     RVMHGDQVMVRIDRKQRGGSEAHIVQIIERGQKRLLGTYEEIAGRPFLVPMDARIGAM
                     PLKSAGAKLSQGKVIAAEISRFASAMSPPEATVVQVMGDPNDPEVQAQSIIFRFGLSP
                     SFPAEVHREVKNAAYEIDAAEIARREDLRALPIVTIDGENARDFDDAVYVRRKDQGYE
                     LFVSIADVAHYVQPNTALDQEAYGRATSVYFPDRAIPMLPEALSNGICSLNPGEDRLT
                     KTAWIEYNSKGEAIGSRFFDSVIQSHARMTYTNVRRILVDQDPECLENYRDLVDGFKL
                     MEELALAIYANRKARGNLDFDLPEAEIVLDLQGLPEDIVRAERNIAHRIIEEFMIAAN
                     EAVARQLTAKNFPTLYRVHAGPDEDALEALEPFLLSLGYRLPQDKENLPPLELQRLLE
                     SARGKPEERVINRVLLRSMKQAVYQPENIGHFGLASACYTHFTSPIRRYPDLIVHRML
                     GRV"
     gene            complement(1819..2100)
                     /locus_tag="VE170_10395"
     CDS             complement(1819..2100)
                     /locus_tag="VE170_10395"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HYJ15888.1"
                     /translation="MDLQEIYLEIAPEHIAYIKFIFESYEEVGIIRTVDRKKSIIVLL
                     AMPDFGDAARAIIDSLKQDIPLAEIPRPADLTDDWFMLELAGAMSTAEK"
     gene            complement(2112..2831)
                     /locus_tag="VE170_10400"
     CDS             complement(2112..2831)
                     /locus_tag="VE170_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HYJ15889.1"
                     /translation="MQGALEIKGANLQPLLTSLLPLFKDRLDGNISGHTLYDVTLSDG
                     GMMAQSLKATGATTIERGVIKDFNLLSQLLLRGSGSSVSEATKERLPAALIELAKHND
                     TRFDALKSDFTVDNGRISSANLSLSTPDYTVTGAGWFGLDLTTRWNGLLVLSPRLTQE
                     IQRDYRWIRHLLDRRGSLAIPFRVDGTIPNVRIRIENRNLSQVLRGSQPRDKDRDGDD
                     DRPPKEEKGWLPDALDRFLNR"
     gene            complement(2847..>3569)
                     /locus_tag="VE170_10405"
     CDS             complement(2847..>3569)
                     /locus_tag="VE170_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15890.1"
                     /translation="RNQRLTIHESELTLGAAKTVFSGVVQDIDAPRLHLRLRAEDAPL
                     ARWTPLSNGMVTAQSGRASWHLAIERAPAAAPTRWHIDGQLTIADGEVHGRDNRAKLE
                     QLNTHVTFLGQQARIEHASFRLGAAQIALTGFIPNLVEPKLDFQLTAAEFDLADVPVL
                     AISRPLRLQQFRIKGRAQSQSEALSVNGDAAALQGNFDGLPVSDFRSAFSWSAAGLRF
                     KDLTFRAAQGWFRSDGVQTAAA"
BASE COUNT          661 a          998 c         1134 g          776 t
ORIGIN      
        1 acgcggccga gcatgcgatg gacgatcaga tcgggatagc gccgaatcgg cgacgtgaag
       61 tgggtgtagc acgccgacgc cagaccgaag tggccgatgt tttccggttg atagacggcc
      121 tgtttcatcg agcgcaggag cacgcggttg atcacccgct cttccggttt gccgcgggcg
      181 gattccaaca gtcgctggag ctccagcggc ggcaggtttt ctttatcctg gggcagacgg
      241 tagcccaggc tcaagagaaa aggctccagc gcttccagtg cgtcttcatc cggtccggca
      301 tggacacgat agagcgtcgg gaaatttttg gccgtgagtt gccgcgcgac ggcttcgttg
      361 gcggcgatca tgaattcttc gatgatccga tgggcaatat tgcgctcggc ccggacaatg
      421 tcttccggca agccttgcag atcgaggacg atctcggcct ccggcaggtc gaagtcgaga
      481 ttgccgcgcg ctttgcggtt ggcgtaaatc gccagcgcga gctcttccat cagtttgaag
      541 ccgtcgacta gatctcgata attctccaga cattcgggat cttggtcgac caggatgcgc
      601 cggacattgg tgtaagtcat gcgcgcatgg ctttggatta ccgagtcgaa gaagcggctg
      661 ccgatcgctt cgcctttgct gttatattcg atccaggcgg ttttggtcag acggtcttcg
      721 ccgggattca agctgcagat gccgttggac agcgcttcgg gcaacatcgg gatcgcccga
      781 tccggaaaat aaacgctggt ggcacggccg taggcttctt ggtcgagcgc ggtgttcggc
      841 tggacgtagt gagcgacgtc ggcgatggaa acgaaaagct cgtaaccttg atccttgcgc
      901 cgtacataaa cggcatcgtc gaagtcgcgg gcgttctcac cgtcgatggt gacaatcggc
      961 agcgcgcgca ggtcttcgcg ccgggcgatc tccgccgcgt cgatttcata ggcggcgttt
     1021 tttacttcgc ggtgcacttc cgcgggaaag ctgggcgaca ggccgaagcg gaaaatgatc
     1081 gattgcgcct gcacctcggg gtcgttgggg tcgcccatga cttggactac ggtggcttcc
     1141 ggcggcgaca tggcgctggc gaagcggctg atctctgcgg cgatgacttt gccttggctc
     1201 agtttcgcgc cggcgctctt gagcggcatg gcaccgatgc gcgcgtccat cggcaccaag
     1261 aaaggtcgac cggcgatctc ttcgtaggta cccagcagcc gtttttgtcc gcgttctatt
     1321 atctggacga tatgggcttc gctgccgccg cgctgtttgc ggtcgatgcg caccatcact
     1381 tgatcgccat gcatgacccg gcgcatttca cggcggttca agtaaacgtc atcggagccc
     1441 ttgtcttcca ggatgagaaa gccgtagccg tcggggtggg catggacacg accgcgcagg
     1501 agattttgtc gatcgggtag cgcgtaatga ttttttttga gccggactag gcggccgtcc
     1561 cggcagagct ggcgcagggc gtcgatgatc gcttgtaaat cgccgcgttc aatgccggcg
     1621 cggcgctgca cctcggccgg cgtgaaacgg gcgcgcggtt gtttgttgaa cagcccgagg
     1681 attttttctt gtgtcttgtc gatcatggat gttgcgcgag cttctactgg aatccgcggg
     1741 atttcaatct ctcagggtcg cgtcgttatc ggacgtccgc ttgtgctttg gacggcggat
     1801 ggatcgagta ggctggcctt acttttccgc cgtcgacatc gcgccggcca attccagcat
     1861 gaaccaatca tcggtcaggt cggcgggccg gggtatttcc gccagcggga tatcctgctt
     1921 gagcgagtcg atgatcgcgc gcgcggcgtc gccgaagtcg ggcattgcca gcagcacaat
     1981 gatcgatttc ttccgatcca cggtgcgaat gatgccgact tcttcgtagg actcgaaaat
     2041 gaatttgata taggcgatat gctcgggagc gatctctaaa tagatttctt gtaaatccat
     2101 agcggttccg actagcggtt gaggaaacga tcgagagcgt cgggaagcca acccttttct
     2161 tccttgggcg ggcggtcgtc gtcgccgtcg cgatccttgt cgcgcggctg cgagccgcgc
     2221 aatacctggc tcaagttacg gttctcgatg cgaatcctga cattgggtat ggtgccgtcg
     2281 acgcgaaacg ggatcgccaa gctgccgcgg cggtccagca gatggcgaat ccagcgataa
     2341 tcccgttgaa tttcctgagt caagcgcggc gacagcacca acaggccgtt ccatcgagtg
     2401 gtaagatcca agccgaacca gccggcgccg gtgacggtat agtccggcgt cgagagactc
     2461 aagttggcgc tgctgatgcg gccattgtcg acggtgaaat cggacttgag cgcgtcgaag
     2521 cgggtgtcgt tatgtttagc aagttcgatc agggctgccg gcaggcgctc cttggttgcc
     2581 tccgacacgc tggagccgct gccgcgcagg aggagctgcg acaataaatt aaagtccttg
     2641 atgacaccgc gctcgatggt cgtagcgccg gttgccttga gcgattgcgc catcatgccg
     2701 ccgtcgctca gggttacatc gtaaagcgtg tgaccgctga tattaccgtc gagacgatct
     2761 ttgaacagcg gtaacaagct ggtgagcaac ggctgcaagt tggcaccttt gatttccaat
     2821 gcgccctgca attgcgcgct atttcttcaa gcggcggccg tttgaacgcc gtccgagcga
     2881 aaccagccct gggcggcgcg aaacgtcagg tccttgaaac gcagtccagc cgccgaccag
     2941 gagaaggccg agcgaaaatc gctcacgggc aaaccgtcga agtttccttg cagcgctgcc
     3001 gcgtcgccgt taacgctcag cgcctcgctt tgagattgcg cccgtccctt gatcctaaac
     3061 tgctgcaggc gcagcggccg ggagatcgcc aagaccggca catcggctaa gtcgaactct
     3121 gcggcggtca attggaaatc gagttttggc tcgaccagat tcggtatgaa gccggtgagc
     3181 gcaatctgcg cggcgccgag ccgaaaactg gcgtgctcga tacgcgcttg ctggccaaga
     3241 aaagtgacat gggtattcaa ttgttcgagc ttggcgcggt tatcccggcc gtggacttcg
     3301 ccatcggcga tggtcaattg gccgtcgata tgccaacgcg ttggcgccgc tgccggtgcc
     3361 cgttcgatgg cgagatgcca gctggcgcgg ccggattgtg ccgtgaccat gccgttactg
     3421 agcggtgtcc agcgcgcaag gggagcgtct tcggctcgca atcttaaatg caaccgcggc
     3481 gcatcgatgt cttgaaccac accggaaaat accgtcttgg ccgcgcccag ggtgagctcc
     3541 gactcgtgaa tcgtcagacg ctggttgcg
//
LOCUS       DASQKS010000401         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6108079, whole genome shotgun sequence.
ACCESSION   DASQKS010000401 DASQKS010000000
VERSION     DASQKS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6108079"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VE170_10410"
     CDS             <1..973
                     /locus_tag="VE170_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15891.1"
                     /translation="LILSLLGSGFFASGSDAQVTRLKTSYSALTANMAAYWLAKEAKI
                     FEKHGLSVDVVLIESGVTTVQALTAGETQIAMGGGTVAVSSNLAGSDIVSISSIESRM
                     PYALLAQKEIKTIDQLKGKRLAVSRFGSASDLAARLILQRYGLTPDKDVTILQTGGTS
                     TRLSALSKRSVDCTVLTPEFFNLGKKAGFTILVDPTQYDIPFPQLEVITSRAYLKAQP
                     EVITRYMRAIVEGIYMVKQNPEPSIRALSKYLKIDDREALEEVYKLYKELYPQIPHPS
                     PAAIQTQLTWMAEKDPRAKAAKPEQFIDGTIMREIEKSGFVAKLYQK"
     gene            complement(1001..1951)
                     /locus_tag="VE170_10415"
     CDS             complement(1001..1951)
                     /locus_tag="VE170_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15892.1"
                     /translation="MRRVFALLLVVSLETTVQAADKIRIGMPSDAGQFTLPLAQKRGY
                     LRDEGFDAEIITITGPVANVALANGDIDYFTGFGSAIRAMLQGQLPTRIVVCYRPVAH
                     FVLVSRPENKSIKDLKGKTVGASPGTAPDLLARLMVRHYGLDPDKDMKFISSPGGETA
                     LVRMSQGLIDAQAMPVPWDYRSKKLGLTVLARAEDLFTYPISGLIAHTRKIKERPDEV
                     RRVIRAGIKANRYMRTNRDGTIEVLMSTYRLDKETATALYSSLIKGFNEDGNLPEDGL
                     RRLIDGVKSVIKVDREIALSEVADLSILREVQRELGIKGK"
     gene            complement(2376..3800)
                     /locus_tag="VE170_10420"
     CDS             complement(2376..3800)
                     /locus_tag="VE170_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYJ15893.1"
                     /translation="MMGSDICLTSARTLAGMIRARKISAREVMAAFCAQIARLNPKIN
                     AIVAKLPDAACLALADEADRRMVRGEAVGPLHGLPFAFKELDPVVGFPMTRGSLIFKN
                     FMPTEDSVLAERLRHAGVVPIGKTNISEFGMGSHSYNQVYGTTLNPYDLTKSAGGSSG
                     GAGAALAAGLVPLASGSDLGGSLRNPANFNNIVALRPSVGLVPAAPVALPFLGFTVKG
                     PMARSVADTSFLLSIISGADARDPACYPSDPLVFAAPLERDFKDVRLAWCPDLGHLPL
                     DRRVRAVLAAQRQTFVDLGCAVEDAVPDLTGADEVFLTLRAWHYWHTLGSLLERHRHK
                     MKPEAVWQIELGRNLTGDDIARVMQLHGELMQRMRRFQEQYEFIVCAVNQLPPFDASI
                     DWPREIEGVRMATYLDWMKSAYWITSTFCPAISVPAGFSPEGLPVGIQLIGRYRDDFG
                     LLQLAHAFESATGFAKRRPEISRQ"
BASE COUNT          867 a         1157 c         1064 g          781 t
ORIGIN      
        1 tctgatctta agtttgctcg gcagcgggtt ttttgccagc ggcagcgatg cccaggttac
       61 ccgcctcaag acctcctaca gcgcactgac ggccaacatg gcggcttact ggctcgccaa
      121 ggaagccaag atcttcgaga agcacgggct cagcgtcgac gtcgttttga tcgagagcgg
      181 cgtcaccacg gtgcaggcac tcaccgccgg tgagacgcaa atcgccatgg gcggcggcac
      241 cgtggccgtc agcagcaatc tggcgggcag cgatatcgtg tcgatctcga gcatcgagag
      301 ccgtatgccc tacgcgctgt tggcgcagaa agaaataaag accatcgacc agctcaaggg
      361 caagcgcttg gcggtgtccc gctttggcag cgcatccgat ctggcggcgc gtttgatcct
      421 gcagcgttac ggattgacgc ccgacaaaga tgtcaccatc ttgcaaaccg gcggcacgag
      481 cacgcggcta tcggcgttaa gcaagcgcag cgtcgactgc accgtgttga cgccggaatt
      541 ttttaatctc ggcaagaaag ccggcttcac catcctcgtc gacccgaccc agtacgatat
      601 ccccttcccg caactcgaag tgatcaccag ccgcgcctat ctgaaagccc agcccgaggt
      661 gatcacccgc tacatgcgcg ccatcgtcga aggcatctat atggtcaaac agaacccgga
      721 gccgtcgatc cgggcgctga gcaagtatct gaagattgac gaccgcgaag cgctcgaaga
      781 agtttacaag ctttacaagg aactctatcc gcagattccc cacccctcgc ccgcggcgat
      841 ccaaacccag ctcacctgga tggcggaaaa agatccccgc gccaaggcgg ccaaaccgga
      901 acagttcatc gacggcacga ttatgcgcga gatcgaaaaa agcggctttg tggccaagct
      961 gtatcaaaag taacaacgtc tgtagcaggt tactactaag ttacttgccc ttgatgccca
     1021 gctctctctg cacctcgcgt agaattgaaa gatcggcgac ttcgctcaag gcgatttccc
     1081 ggtcgacttt aatcaccgac ttgacaccat cgatgagtct gcgcagtccg tcttccggta
     1141 gatttccgtc ttcgttaaag ccctttataa gcgaactata cagcgcagtc gccgtctctt
     1201 tgtcgagtcg gtaggtactc atcaatactt cgatggtgcc gtcgcgattg gtgcgcatgt
     1261 agcggttggc tttgatgccc gccctgatca ctcggcgaac ctcatcgggt cgctctttga
     1321 tcttccgggt atgagcgatc aagccgctga tcggataggt gaatagatct tccgctcgcg
     1381 ccagaacagt caaacccaat ttcttactac gataatccca gggaaccggc atcgcctgag
     1441 catcgatcag cccttgactc atccgaacca gagcggtctc gccgcccgga ctactgataa
     1501 atttcatgtc tttatctggg tccaagccat agtgccttac catcagccgc gcgagcagat
     1561 caggcgcggt accgggcgaa gcgccgacag tcttgccttt gagatcttta atggatttat
     1621 tctcgggtcg gctgaccaat acgaaatgcg ccacggggcg atagcaaacc acgatcctgg
     1681 tcggcagctg cccctgaagc atggcgcgaa tcgccgagcc aaagccggtg aagtagtcga
     1741 tgtcgccgtt tgccagcgcc acattcgcca ccggccccgt gatcgtaata atctcggcat
     1801 cgaatccttc gtcgcgcaag taaccacgct tttgcgccaa gggaagcgtg aattggccgg
     1861 cgtcggaagg cataccgata cgaatcttgt ccgccgcctg tactgtagtt tcgagcgaaa
     1921 caaccagcag taacgcaaag actcttctca tggatttgcc ctccggatag attgcctggc
     1981 ttggggccaa ggaatcagcc ctgtgccgat aatcaaacgc gtctcgggag cccttacata
     2041 accggccgcg tttcgataac ccaaaagatc gattccgtcg gaattacttt tgttaatttc
     2101 aaaccaattg tctcgcagag cgcttgatac tcagtcaggg tgcgttccgg tccgccggtc
     2161 atccaaatgt tccggcatga tcctctcgat gagcattaga cgggagtccg gcgccatccc
     2221 cggcgggcct ggttcatccg ccgcaatacc gctaccgagg attcagcgga actgtcgcct
     2281 tgtctggttt gattcatcga gcacccctat gcattcggag ttcatacttg ccgggtcaaa
     2341 agcctaatta ctgtaacaag acatcttgcc tgaaatcact gccgagagat ctcgggccgg
     2401 cgtttggcaa agccggtggc actctcgaaa gcgtgggcaa gctgcaacag accgaaatcg
     2461 tcgcggtaac ggccgataag ctgaattccc actggcaatc cctccgggct aaatccggca
     2521 gggacggaaa tcgccggaca gaatgtcgaa gtgatccaat aggccgactt catccagtca
     2581 agataggtcg ccattcgcac accttcgatc tcgcggggcc aatcaatgct ggcgtcgaaa
     2641 ggcggcagct gattcacagc gcagacgata aattcgtatt gttcttgaaa gcgccgcatg
     2701 cgctgcatga gctcgccatg cagctgcatc actcgcgcga tatcgtcgcc agtcaaattg
     2761 cgccccaatt ctatttgcca caccgcttct ggtttcattt tatgacgatg acgttcaagc
     2821 agcgatccga gggtgtgcca gtaatgccac gcgcgtagag tcaaaaatac ttcatcggcg
     2881 ccggtcaaat ccggcacggc gtcttccacg gcgcagccga gatcgacgaa tgtctgacgc
     2941 tgagcagcca acaccgcgcg cactcgccgg tcgaggggca ggtggcctag atccggacac
     3001 caagcaagcc gaacgtcttt gaaatcgcgc tccaacggtg cggcaaacac aagcgggtcg
     3061 gacgggtagc atgccggatc gcgcgcatcg gccccggaga ttatgctcag caaaaacgac
     3121 gtgtccgcta ccgagcgcgc catcggccct ttgacggtaa agccgagaaa cggcagcgcc
     3181 accggtgcgg ctggcaccag tccgacactc ggacgcagcg cgacaatgtt attgaagttt
     3241 gccggattgc gcagcgaacc gccgagatcg ctgccgctcg ccaatggcac caggcccgcc
     3301 gccaatgccg cgccggcacc gccgctcgat ccaccggcgc ttttggtcaa atcgtaggga
     3361 ttgagggtcg tgccgtagac ttggttgtag ctatgcgatc ccatgccgaa ctcggagatg
     3421 ttggttttgc cgatcggcac gacgccggca tggcgcagcc gttcggcgag aaccgagtct
     3481 tcagtgggca tgaagttttt gaatatcaac gacccgcggg tcatcggaaa accaacgacc
     3541 gggtcgagtt ctttgaaagc gaaaggcaag ccatgcagcg gcccgacagc ttcgccgcgg
     3601 accattcgcc ggtccgcttc gtccgccaag gcgagacacg ctgcgtcagg cagcttggcg
     3661 acgatggcgt tgatcttggg attgagccgc gcgatctgcg cgcaaaacgc cgccatgact
     3721 tcgcgcgccg atatcttgcg agcgcgtatc ataccggcga gagtccgggc gctggttaag
     3781 cagatatcgc tgcccatcat tgggaaaaac tccagctcta acgtaagagc gaatgaatcg
     3841 tatacatgaa ctcgcggtag agatcagcg
//
LOCUS       DASQKS010000402         5313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1883487, whole genome shotgun sequence.
ACCESSION   DASQKS010000402 DASQKS010000000
VERSION     DASQKS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883487"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VE170_10425"
     CDS             complement(<1..1108)
                     /locus_tag="VE170_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ15894.1"
                     /translation="MDQDIISRLKQRIIAAGFDAIVAMSPENVTYVSGFVVPSQSLMR
                     WRHAACIVTANGRISMVAIDMEATTVKAHAGIDDLRIYREFSDDPMEKLAEALRDLKL
                     AGSKVAIEMEYLPARDFATLQKSLPGVQWLAADGVFNKARQIKTPRELALLRSLSKLT
                     DNAIGTALRSATIGMSEMEMAGTLLTTLFAGGAESYKLMIIASGERSEFPNVGPSERK
                     LKHGDIIRMEIFGQKSGYLTGVCRTAVVGEATPEQYKIWSNLIECKYLVMDLIKPGAS
                     CAEVYRKFLEKFSQLGFEPISFVAHGIGLHLHEEPYMGRYGNEIIEAGMVGAFEPLVY
                     IPGRFGMQNKDMFCVIDEGCELLSDVTPTDTLLRV"
     gene            complement(1177..2181)
                     /locus_tag="VE170_10430"
     CDS             complement(1177..2181)
                     /locus_tag="VE170_10430"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYJ15895.1"
                     /translation="MSGIFISYRREDSAPYAGRLCDRLSEHFGADQVFMDVDDIPPGA
                     DFAAHIGAKIGACNALLAVIGKQWLTARTGAGQLRLSEPNDWVSREIALALQRRILVI
                     PVLVEGTSMPKADALPRDLRPLAQRNAVIIGDHDFQADTAKLIKALEALPALGKQTPQ
                     ASDNWKAELRQRLRRRLVWKIPLIILLVSFAIWWQWRKDANPPLSTAEQAAAAHVAAA
                     VSGTWRGDVTYPWGAKYSERFLFNAEGAKLFGVATFLASKRAIEDGQIVGETILFRVR
                     YQEVGDGDARTRLNHYEGKLSGNQISLRVFDDKGGAPVEFDLTRIVDGGGTDVGAAKR
                     "
     gene            complement(2297..3742)
                     /locus_tag="VE170_10435"
     CDS             complement(2297..3742)
                     /locus_tag="VE170_10435"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15896.1"
                     /translation="MPKSLRTFLDDFRRLYPNEVVSIAKTVNPLTYDITAIVKHLGAL
                     KKFPVLVFENPLNARGQPTDMKVVMSAENSQKKIQVALGLPADIDRAEMARECLRREA
                     GKVGPVIVSKDAAPVKDIIQTGEQVDLYLLPLLKHHEMDGGPYLDMSSVAKDRKNGIY
                     NCSYHRMEVKDRNHTGFLMTLQHMWRIFRGYEEAGEECPVATVIGHHPAYQMGACYGG
                     PFDISEYDVIGGYMQEPLRLAPSETWGERLLVPADAEIVIEGALLPGKRMVEGPFGEV
                     NGYLGVQGYQQSAHYEVRAITRRRAALHTSILTPEGDKPWMDLAREGAYLRRAREAVP
                     GVLAVCTSGRHALLNVFISMKKISEGDPGRAAAAVLTWDWCKNVFVFDEDINVYDPTE
                     ILWAIATRVQPHLQVSIIKEIMRGSLIDPSMVDARKTSAMIVDATKPLDRPFSPVSKC
                     PDEAMARIHIEDFVPGEVLQHIPIDRTTYWA"
     gene            3946..5028
                     /locus_tag="VE170_10440"
     CDS             3946..5028
                     /locus_tag="VE170_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15897.1"
                     /translation="MGIIERTYGTAKNAPTNELLISSDSHVIEPAGLWQKELPKPYKD
                     RAPDFGGQRPNDTPGGAQDKNKRVAEMTADGVSAEVLYPTHGLKCLSLDDHELEGACC
                     RVYNDWLIDYCSAAPDRLIGISMLSMYDTDGAVKELERCRKAGMRGSVIWQVPPPELP
                     FSSSHYDKFWAASQDLDMSVNLHILSGHGYSRSRSVGLGDTPTGMTAEHNSVNMKLFQ
                     SVDSLYELIYSGVLERFPKLKVVLVENELGWIPFVLEQWDYYFKRHGANRKDLAIKKL
                     PSEYFHGQIYTTFFNDAVGGHILNWWGEDNCMWSNDFPHGNSTWPKSREVVARDLGDL
                     PAKLRAKLVRENVAKVYNIPLPTQIQ"
BASE COUNT         1160 a         1590 c         1412 g         1151 t
ORIGIN      
        1 ccactctcaa caaagtatct gtcggcgtca catcggaaag caattcgcag ccctcgtcaa
       61 tcacgcaaaa catgtctttg ttctgcatgc cgaagcgccc ggggatataa accaaaggtt
      121 cgaaagcgcc gaccatgccg gcttcgatta tctcattgcc atagcgcccc atgtacggct
      181 cttcatgcag atggagaccg atgccatggg cgacgaagct gatcggctcg aagcccagct
      241 ggctgaactt ctcaagaaat tttcggtaca cttccgcgca gctcgccccg ggcttgatca
      301 aatccatgac cagatacttg cactcgatca aattagacca gatcttgtac tgctccggcg
      361 tcgcctcgcc aaccaccgcg gtgcggcaaa cgccggtgag gtagccgctc ttctggccga
      421 agatttccat gcgaatgata tcgccatgtt tcagcttacg ctcgctcggt ccgacgttgg
      481 gaaactcgct gcgctcgccg gaggcgatga tcatcaactt ataactctcg gcaccgccgg
      541 caaacagcgt cgtgagcaaa gtgcccgcca tctccatctc gctcatgccg atcgtggcgg
      601 agcgcaacgc cgtgccgatg gcgttatccg tgagcttgct cagggagcgc agtaaggcaa
      661 gctccctggg cgtcttgatc tggcgcgctt tgttgaagac cccatccgcc gccagccatt
      721 gcaccccggg caaactcttt tgcagcgtgg cgaaatccct tgccggtaag tactccatct
      781 cgatggccac tttactgcca gcgagcttca agtctcgtaa cgcctcggcc agcttctcca
      841 tcggatcgtc gctgaactcg cgataaatgc gcaggtcgtc gatgccggcg tgcgccttaa
      901 ccgtcgtcgc ctccatgtca atggcgacca tagaaatcct gccattggcc gtgacgatgc
      961 aagctgcatg gcgccagcgc atcagcgact gggacggcac gacgaaaccc gagacatagg
     1021 tgacgttttc cggcgacatg gcgacgatgg catcgaaccc cgcggcgatg atccgttgtt
     1081 ttaaccgtga gattatatct tgatccatga agttatcggc gagaatatcc actggataac
     1141 ggggggtcaa gtcaagctac gcgggattgc tcgagcctat cgcttggcgg caccgacatc
     1201 cgtgccgccg ccatcgacga ttctggttaa atcgaattcc accggtgcgc cacctttgtc
     1261 gtcgaacact cgcaagctga tttggtttcc cgagagcttg ccctcgtagt ggttcaagcg
     1321 ggtgcgcgcg tcgccgtcgc ccacttcttg gtatctgact ctgaatagaa tcgtctcgcc
     1381 aacgatctgc ccgtcttcga tggcgcgctt agaggcgagg aacgtcgcga cgccaaatag
     1441 cttcgctccc tcggcgttga acaagaatcg ttcgctgtac ttggcacccc atgggtaagt
     1501 cacatccccc ctccaggtgc cagacactgc cgcggcgaca tgggcggcgg ccgcttgttc
     1561 ggcagtgctc aacggcggat tggcatcttt tcgccactgc caccagatcg cgaagctgac
     1621 cagcaaaata atcaagggga tcttccacac cagccgccgc cgcaaccgtt ggcgtaattc
     1681 agctttccag ttatcgctag cctgcggcgt ttgtttacct agtgccggca gcgcttcgag
     1741 cgcctttatc agcttcgccg tatcagcttg aaaatcgtga tcgccaatga tcaccgcatt
     1801 gcgctgggcc aagggccgca agtctcgcgg cagcgcatcg gctttgggca tgctcgtgcc
     1861 ttcgaccagc accggaatga cgaggatgcg gcgctgtaag gctagtgcta tttcacggct
     1921 gacccaatcg ttgggttcac tgaggcgcaa ctggcccgcg ccggtgcgtg cggtgagcca
     1981 ctgtttcccg atcaccgcta acagagcgtt gcaggcgccg atcttcgcgc cgatatgggc
     2041 ggcgaagtcc gcgcctggcg gaatgtcgtc gacatccatg aagacttggt ctgcgccaaa
     2101 atgctcgctc aaccggtcac acaaccggcc ggcgtagggc gcgctgtctt cgcgccgata
     2161 actgatgaag ataccggaca tgattcacaa tactattcgt tcaagggttc aaaagttcaa
     2221 ggttcaatcg gaaattcagc gattttccga tcgtccgacc ttgaactttt gaacccttga
     2281 accttgaacg atttgtttac gcccaatacg tcgtcctgtc gatgggaata tgctgcagga
     2341 cttcgcctgg cacaaagtct tcgatatgaa ttcgcgccat ggcttcgtcg ggacatttcg
     2401 acacaggtga gaacggtctg tccaacggct tggtagcgtc gacgatcatc gcggaagtct
     2461 tgcgcgcatc gaccatggac gggtcgatca aactgccgcg catgatctcc tttatgatcg
     2521 aaacctgcag gtgcggctgc actctcgtcg cgatcgccca gaggatttcc gttggatcat
     2581 aaacgttgat gtcttcatcg aagacaaaaa cattcttgca ccaatcccaa gtcagtaccg
     2641 cggcggcggc acgtccggga tcgccctcgg agatcttttt cattgaaatg aaaacgttca
     2701 acagcgcgtg cctgccgctg gtgcagaccg cgagcacgcc gggcaccgct tcacgcgcgc
     2761 ggcgcagata cgcgccctcg cgcgccaagt ccatccacgg cttatctcct tcaggcgtaa
     2821 gtatcgacgt gtgcaacgca gcgcgccgcc gggtgatggc gcgcacttcg tagtgcgccg
     2881 attgctgata accctgcacg cctaggtaac cgttcacttc accgaacggt ccttcaacca
     2941 tgcgcttgcc gggaagcaag gcgccttcga tgacgatctc cgcgtccgcc ggtaccagca
     3001 gtcgttcacc ccaagtctcc gacggtgcca ggcgcagcgg ctcctgcata tagccgccaa
     3061 tgacgtcgta ttcactgatg tcgaacggcc cgccataaca cgcgcccatt tgatacgccg
     3121 gatgatggcc gatcaccgtg gccaccggac attcctcgcc agcctcttca taaccacgaa
     3181 aaattcgcca catatgttgc agcgtcatca agaagccggt gtggttgcga tccttcacct
     3241 ccattcgatg ataggagcag ttgtagatac cgtttttgcg gtctttggcc acgcttgaca
     3301 tgtcgagata cggtccgcca tccatctcgt gatgcttgag cagcggaagt aggtagagat
     3361 cgacttgctc gccggtctga atgatgtcct tcaccggggc cgcatcttta ctgacgatta
     3421 ccgggccgac cttgcccgcc tcgcgacgta aacactcccg tgccatctcg gcgcgatcga
     3481 tgtccgccgg caaacccagc gcgacctgaa tctttttctg cgaattctcc gcgctcatca
     3541 ccaccttcat atcagtcggc tggccgcggg cgttgagcgg attttcgaag accaaaactg
     3601 gaaacttctt gagtgcaccg agatgcttga cgatcgccgt gatatcatag gtcagcggat
     3661 tgacggtctt ggcgattgac acgacctcgt tcggataaag ccgccggaaa tcgtctagaa
     3721 aagtgcgcag cgatttaggc atgattgagg actcttcgtt gttacctttt gattcgaacg
     3781 tattcgccga ttattatgca actaccgtcg ccttcacaag cgatcaggtt gctcacttca
     3841 ttccggcgat acccgagtgc caatttttga taatccgctg gagccgcctc ttgcaccgta
     3901 ccctgcgcct gagttatttg aatggaaaga ggaggatcaa atcccatggg cattattgaa
     3961 cgaacttacg gcacagcgaa gaatgcgccg accaatgaat tgctaatctc ctcagactcg
     4021 catgtcattg agccggcagg actatggcaa aaagaactgc caaaaccata caaggaccgg
     4081 gcaccggact tcggcggtca acgcccgaac gatacgcccg gcggtgcgca ggacaaaaac
     4141 aagcgcgtcg ccgaaatgac tgccgacggc gtcagcgccg aagtgctcta tccgacccat
     4201 ggtctgaagt gcttgagcct cgacgatcac gaattagagg gggcatgctg ccgagtctac
     4261 aacgattggc tgattgacta ttgcagcgcc gcaccagacc ggctcatcgg catatcgatg
     4321 ttatcgatgt acgacaccga cggcgcggta aaagaactgg agcgctgccg caaagccggc
     4381 atgcgcggct cggtcatctg gcaggtgccg ccgcccgagt tgccattttc ctcgtcgcac
     4441 tatgacaaat tttgggcggc ctctcaggat ctcgatatgt cggtcaacct gcacatcttg
     4501 agcggtcacg gctacagccg cagccgttcg gtaggtctcg gtgacacgcc aaccggcatg
     4561 actgcggagc acaatagcgt caacatgaaa ctgtttcagt cggtcgatag cctatacgaa
     4621 ttgatatatt ccggagtgtt agaacgcttt cctaaactaa aagtagtgct cgttgaaaac
     4681 gaactcggct ggattccgtt tgtcctggag cagtgggact actatttcaa acgccacggc
     4741 gccaatcgca aagacctggc gatcaaaaag ctgccgagcg aatactttca cggccaaatc
     4801 tacaccacct tcttcaacga cgccgtcggt ggccacattc tcaactggtg gggcgaagac
     4861 aactgcatgt ggtccaacga tttcccacac ggcaattcca cctggccaaa gtctcgcgag
     4921 gtcgtcgccc gggacctggg cgatctgccg gcaaagttgc gcgcaaagct ggtacgagaa
     4981 aacgtcgcga aggtctacaa catcccattg ccaacgcaga tccagtaacc taaaaaacac
     5041 ctcggtaccg gttcgcttcg gggggccggt actgatccat cctttctcaa atcccatttg
     5101 agccgcggcg aaaccgtgcg atagagacca gaaccaactc tgaccccatg ccctgaggtc
     5161 gttttcccga tgattccaga ggcaagttga ccattatagc tataatagct aatatggtcg
     5221 atacggggga ttgccatgtc atctctagga ttcagaaata gccggggcgc ctgggttacg
     5281 gtacccaccg tcgccgccac cagagttaag aac
//
LOCUS       DASQKS010000403         8875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3990884, whole genome shotgun sequence.
ACCESSION   DASQKS010000403 DASQKS010000000
VERSION     DASQKS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3990884"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VE170_10445"
     CDS             complement(<1..607)
                     /locus_tag="VE170_10445"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HYJ15898.1"
                     /translation="MYQEDTIAAIATPPGEGGLAVVRISGPDAEKVALRIFLRSNNRN
                     DKLRSHTLYHGRISDPQDAQPIDEVLLTIMRKPHSYTGEDVVEVHCHGGNFLVREILA
                     LILTQGARQAEPGEFTKRAFLNGRVDLAQAEAVLDLIRARTTQGAQLALNQASGELSK
                     YVAELREELLNIMVQVEAAIDFPEEEIELLQRHRLVAKIFAL"
     gene            complement(850..2505)
                     /locus_tag="VE170_10450"
     CDS             complement(850..2505)
                     /locus_tag="VE170_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15899.1"
                     /translation="MIGRSFLYLNLPSRRIVALVTMLSFLFAPLLLALAMDATRNGVL
                     PQRLPLFPADNWWNLDISNWPVDQNSPSYINFINNGGTRKLHPDFGGNSGTGYGIYGL
                     PYALLNNVTDADRKPVQFQYWDESDGVNLQTGASVPFYPIPDAAASQPYWVEGGDPGN
                     IDKRSSQDRHLLLVDRERNHLYELYNVFYNATLGKWYAGSGAFFDMNTNNRRPDTWTS
                     ADAAGLAILPGLVRYDEVYDPNVTEINHALRVTVRASNGYVYPASHRAGSTLGALPMG
                     ARLRLKNTVNVAQRTSDPNVQKIFRAMQRYGLIVADNGSDMYITGTYDTRWNNGVLNP
                     AFANLSASDFEVIQLGYNPVPAAAALASVTVTPASATGGQSRSGTVTLTSAASATVVV
                     NLTSNMSTAQVPTSVTIPANASAANFTVTTQAVSSLTAGNISASYNNVTKSASISLLP
                     APVTPSAGLASLTLSPTTVSGAAASIGTVTLTAPAPAAGLVVKLSSNIPSRAKVPTTV
                     TVPSGSASASFTVTTVKVNRKFSPTITASYSGVKKTAVLTITR"
     gene            complement(2718..3866)
                     /locus_tag="VE170_10455"
     CDS             complement(2718..3866)
                     /locus_tag="VE170_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYJ15900.1"
                     /translation="MKIADVKTIRLRAEIPAAGQVFSRSGVRNTRSTTLVKVETDDGI
                     YGLGSASGNGELIEFIVARVLKPLLVGMDPTDIDEIWDTAYVRGGHKEFGTRGIGVVA
                     LSGVDIALWDILGKVRGVPLYQLLGGKVRDKVPVYATALYPEEPSKVARRARGFAEQG
                     FHGVKIKVGFDLDQDIRIVRAVRQELGKDFIVMTDANQGYSIDVGIKAAQAFADAGAF
                     WLEEPLFVEDIEGHAILRERGNTPIAVGENLHMTYGFENFILRGAVDFIQPDVARAGG
                     ISEIRKITALAAKHKVPVSFHTWGDGVALAASVHLSAALQDCIVMELDYTYNPLREEL
                     LQEPFKVDNGYLTPSDKPGLGIELNQDALQRFAFAGAEDMAPRQKTLR"
     gene            complement(3976..4410)
                     /locus_tag="VE170_10460"
     CDS             complement(3976..4410)
                     /locus_tag="VE170_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15901.1"
                     /translation="MQASTFKLKTNLLSGGRSHTPLAKSGALTMGLNFYTVGRKNKLH
                     THPGEDHAFVVIDGQATFYDKEGKATVLNKGEGIMLPDGYYYYFESTGTVPLAILRSS
                     AARKDRPAVLRVDVKGDQRMEEEEGFAVADGSAIEGQYWEMK"
     gene            complement(4460..4891)
                     /locus_tag="VE170_10465"
     CDS             complement(4460..4891)
                     /locus_tag="VE170_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ15902.1"
                     /translation="MDAFTYKLETPMITGGRSHIPLADTELMSVGINYYAVGRKNKLH
                     AHFGEDHTFVVLDGQATFYNKEHQPKVLNKGEAIMLPIGTLYYFENSGDKPLALLRVS
                     ALKGKPEFTRVDAEGNKRTAVEDKYITVDGEPIPGQFWQMT"
     gene            5107..6015
                     /locus_tag="VE170_10470"
     CDS             5107..6015
                     /locus_tag="VE170_10470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ15903.1"
                     /translation="MAKIGLAWVTPAPLTKPENVVNFAKKCEAMGCDSMWTIDRIAYD
                     NLEPLTILAAAAGATQKIRLGTSVLLGNTRHPAHLAKIIATLDFISNGRVTIGLGFGS
                     REPDYKAVEIPYEHRGSRAVEQVQLMKRLWTEDNVTHKGRFYNVENLSIGPRPVQKPH
                     PPLWTGGSAEVALKRAGTWADGFISGSAAIADFHVTWDKIASYAAAAGRNPNDIEKAS
                     LAFMAIDDDKAKAVKVIEDYTTRYYGRIRTPVEPVSIVGNPDQCVEKINYFLSKGLDT
                     LIIGVADPDPSQLDLFGEKVLSKFKS"
     gene            6108..7241
                     /locus_tag="VE170_10475"
     CDS             6108..7241
                     /locus_tag="VE170_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15904.1"
                     /translation="MIKAWDADGHVSESEVTFSDKYWDPKLKDRRPTILETGPDGPLY
                     WMIDTRSFPVRSGPYQQAGFPLSKNGVAARVTPGISPNDPVESAEMRSVKARLEQLDR
                     ENTALQVIYPTMFLSYPITYDRELAVAMMRAYNSWVADLASQCPERLKWVTIIDPIDP
                     QASVREIERGKKLGAVGVMLLGVTGDVRVDDPPMEPIWAAANDTGLPVAVHTGHSFRA
                     LGQVNDTHHDKTALSFWLTVQFAFHRVISKGIADRYPNLRIAFLEAGCSWVPALVERI
                     EEYSGFPGARAGVDFHRGYTGKHWPKEYIERGQIYFGFEVDEKLLPFAIEEFGDQCWV
                     YGSDIPHGDRLYGAVDVFLKRDDISDESKRKLLVDNTARFYGL"
     gene            complement(7407..8519)
                     /locus_tag="VE170_10480"
     CDS             complement(7407..8519)
                     /locus_tag="VE170_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HYJ15905.1"
                     /translation="MEQNSANKYFPTVGDGSGLAQTDALYKEELQLALRNRGMPLEAL
                     RYPITPTGLHYLLIHYDIPAVDADGWRLKIAGQVATPLELTLEAIKKRPRQTIPVTME
                     CAGNGRALFAPRRISQPWLLEAIGTAEWTGTPLRTILQEAGIDGAAAEIVFTGLDRGY
                     EGDQVQHYQRSLTVAEATRAEVLLVYEMNGEPLPPQHGFPLRLVKPGWYGMASVKWLN
                     RIEAVAEPFQGYQMVSAYRYAQSAEDPGVVVDVIRARALMIPPGIPDFMTRDRLVDAG
                     AVTLTGKAWAGQGGVSRVEVSVDGGATWSEAQLGESASPFAWRSWTFEWHATAGAHLL
                     QVRASDATGNVQPAVQQWNFGGYGNNGVQRVHVVVR"
BASE COUNT         1859 a         2544 c         2534 g         1938 t
ORIGIN      
        1 ccaaggcgaa gatttttgcg accaggcgat ggcgctggag gagctcgatc tcctcctcag
       61 gaaaatcgat ggcggcttca acctggacca taatattgag cagctcctcg cgcagctcgg
      121 cgacgtactt cgataactca ccgctggcct gattcaatgc caattgtgcg ccctgggtag
      181 ttctggcgcg aatgagatcg agcaccgcct cggcctgggc gagatcgacg cggccgttca
      241 aaaaagcccg cttggtgaat tcgccgggct ctgcttgacg cgcgccttga gtcaagatca
      301 gcgcgagaat ttctcggacc agaaagttgc cgccatgaca atgaacctcg accacatcct
      361 caccggtgta actgtgcggc ttgcgcatga tcgtgagcag cacttcatcg atcggttgcg
      421 cgtcctgcgg atcactgatt ctcccatgat agagagtatg cgaacgaagt ttatcgttgc
      481 gattgttcga ccgcaaaaat attcgcagcg cgactttctc ggcatcgggt ccgctgattc
      541 ggaccacggc gagaccgcct tcgcccggcg gcgtggcgat cgcggctatg gtgtcttcct
      601 ggtacatgga gccgaataat cttaacagct ctccggtcca agacaaccga actcgaaagc
      661 gtgaatctgt tgagaatggc ctggctggct gcccatacca gagattgttt tgtgaaaatg
      721 ccggctagtt gccgtggagc attttcacca aaccattgtc gctgattttc ttgggacgcc
      781 gcgacgggtt ggatggtagg gcaacgcaag cactgtcgag tgatcgacag tgcttgctcg
      841 aagagtttat taccgggtaa ttgtcagaac tgcagttttc ttaacgccac tataggatgc
      901 ggtgattgtt ggcgaaaact ttcggttcac tttgacggtt gtaacagtga aactcgcgga
      961 tgcactcccg gaaggaacgg ttacggttgt cggaactttt gcacggctgg gaatattgct
     1021 cgacaatttc actactaaac ctgcagcagg tgcgggcgcg gtgagggtca ctgtcccaat
     1081 cgatgcggcg gcgccgctga ctgtggtcgg gctgagggtt agcgaagcca gacctgccga
     1141 tggcgttacc ggcgctggca ataaactgat cgatgctgac ttggtcacat tgttgtagct
     1201 tgctgagata tttcccgccg tcaacgaact cacggcttgt gtggttaccg tgaaattcgc
     1261 tgcgctggcg tttgccggaa tggtcaccga cgtcggtacc tgtgccgttg acatgttgct
     1321 cgttaagtta accacgacag ttgctgatgc agcgctggtc aaggttaccg ttcctgatct
     1381 cgattgtcct cctgtagcag acgccggtgt tactgttact gaagcgagcg ccgctgccgc
     1441 gggaacggga ttgtagccga gctgaatgac ttcgaaatca ctggccgaga gattggcaaa
     1501 ggcaggattc aatacgccat tgttccaacg ggtgtcgtag gtgccggtga tgtacatgtc
     1561 cgagccgtta tccgccacga tcaagccgta tctctgcatg gcgcgaaaga ttttctgcac
     1621 gttgggatcg ctggtccgct gcgcgacatt gacggtgttc ttcaagcgca ggcgcgctcc
     1681 catcggcaac gcgcctaggg tcgaaccggc gcgatgagag gccgggtaga cgtagccgtt
     1741 gctcgcccgc actgtgacgc gcaacgcatg gttaatttcc gtcacgttgg gatcatagac
     1801 ttcatcgtag cgaaccagtc cgggaaggat tgccaaaccc gcggcatcgg ccgaagtcca
     1861 ggtgtctgga cggcggttgt tggtattcat gtcgaagaag gctcccgagc ccgcgtacca
     1921 tttccccagg gtggcattgt agaaaacatt gtagagttca tatagatgat tgcgctcgcg
     1981 gtcgaccagc aacagatgcc gatcctgcga gctgcgcttg tcgatatttc ccggatcgcc
     2041 gccttcgacc cagtaaggtt gagaagccgc cgcatcggga attgggtaaa acggcacgct
     2101 tgcgccagtc tgcaaattaa cgccgtcgct ttcatcccaa tactgaaact gcaccggttt
     2161 acgatctgca tcggtgacgt tgttgagcag cgcgtagggc aggccgtaga tgccgtatcc
     2221 ggtgccggaa ttgccgccga agtcgggatg gagctttcgc gtgccgccat tattaatgaa
     2281 attgatgtag ctaggagagt tttgatccac cggccagttg ctgatatcga gattccacca
     2341 gttgtcggcc ggaaacagcg gcagccgctg gggtaatacg ccgttgcgcg tcgcgtccat
     2401 cgccagggct agaagaagcg gggcgaatag gaacgacagc attgtaacta acgctacgat
     2461 ccgacggctc ggcaaattta gatagagaaa tgagcgacca atcatgcttg cctcctcggc
     2521 ggtttgcacg ccgggattta tcgggggcaa gccccatgcc atgaaaccct ggacgcaaac
     2581 tttcagttgt gcgccggaaa gttcctagtt gaccgatcga tgtgggagcg ggttaggtgt
     2641 gattcggcgg attgcccggc agcggtacag atgtgttcgc aaattatcgc aaaccgcgag
     2701 attcgttttt atgccaatta gcgcaaagtc ttctgccggg gcgccatatc ttcagcgccg
     2761 gcgaaggcga agcgctgcag cgcatcctga ttcagttcga tgcccaatcc cggtttgtcg
     2821 ctgggcgtca gataaccgtt gtcaactttg aacggctcct gtaacaattc ttcgcgcagc
     2881 ggattgtagg tgtagtcaag ttccatgacg atgcaatcct gcagcgccgc ggaaagatgg
     2941 acgcttgcgg caagcgctac gccgtcgccc caggtatgaa acgacaccgg cactttgtgc
     3001 ttggccgcca acgcggtgat cttgcgaatc tcgctaatgc cgccggcgcg ggcgacatcg
     3061 ggttgaataa aatccaccgc gccacgcaga atgaaattct cgaaaccata ggtcatatgc
     3121 agattttcgc cgacggcgat gggcgtattg ccgcgttcgc ggagaattgc atgaccttcg
     3181 atgtcttcga cgaagagcgg ttcctccaac caaaaagcgc cggcgtcggc aaatgcctgg
     3241 gccgccttga tgccgacgtc gatgctgtag ccttgattgg cgtcggtcat gacgatgaag
     3301 tctttgccga gctcctggcg cacggcgcgg acgatacgaa tgtcttgatc gagatcgaag
     3361 ccgactttga tttttacgcc gtgaaaccct tgctcggcga aaccacgcgc ccggcgggca
     3421 actttcgacg gctcttcggg atagagcgcg gtggcgtaca ccgggacttt gtcgcgcact
     3481 ttgccgccga gcagttgata gagcggcacg ccgcgcacct tgccgagaat atcccaaagc
     3541 gcgatgtcga cgccgctcag ggcgacgacg ccgatgccgc gggtgccgaa ctccttatgg
     3601 ccgccgcgga cataagcggt gtcccaaatc tcatcgatgt cggtcggatc catgccgacc
     3661 agcaacggct tgagcacccg ggcaacgatg aattcaatca attcgccgtt tcccgaagcc
     3721 gagccgagac cgtagatgcc gtcatcggtc tccactttga ccaacgtcgt cgagcgcgtg
     3781 tttctcacgc cggaacggct gaacacctgt cccgccgccg gtatttccgc gcgcagccgg
     3841 atggttttta catcggcgat tttcatcttt cacctaaagg attcgagctt cgccgctgcg
     3901 cagacgaata cacctgggcg gaaccaccga tgcacgagca aatcaaagca gcgatgacag
     3961 cgccgtcttc acggcctatt tcatttccca gtattgacct tcgatggcgc taccatccgc
     4021 cacggcgaaa ccttcttcct cttccatgcg ctggtcgcct ttgacgtcga cgcgcagcac
     4081 cgccggccgg tctttgcgcg ccgcgctgga gcgcaggatc gccaacggca ccgtgccggt
     4141 gctctcgaaa taatagtaat agccgtcggg cagcatgatg ccttcgcctt tattcagcac
     4201 cgtggccttg ccttctttgt cgtagaacgt cgcctggcca tcgatgacca cgaaggcgtg
     4261 atcctcgccg ggatgggtgt gcagcttatt ttttcgaccg acggtgtaaa agttcagacc
     4321 catggtcagg gcgccggatt ttgccaatgg tgtgtggctg cggccgccgg agagcaaatt
     4381 agttttcaac ttgaaagtcg acgcttgcat atcgttggtt ctccttcgac tatcctcaaa
     4441 gggatgcatc cgcagggcat caggtcattt gccaaaactg ccccgggatc ggctcgccgt
     4501 ccactgtgat gtacttatcc tccaccgccg ttcgcttgtt accttcagcg tccacgcggg
     4561 tgaattccgg cttgcccttg agcgcgctca cgcgcaacaa tgccaacggc ttatcaccgg
     4621 aattttcaaa gtaatatagg gtgccgatcg gcaacatgat cgcctcgccc ttgttaagca
     4681 ccttcggctg atgttcttta ttgtagaacg tcgcctggcc gtccaacacg acaaaggtgt
     4741 ggtcctcgcc gaaatgggca tggagtttat ttttacgtcc cacagcataa tagttaatac
     4801 cgacggacat taattccgtg tcggcgagag gaatgtgact tctcccaccg gtgatcatcg
     4861 gcgtctcaag cttataggta aaagcgtcca taacttcgta cctcctctgt ttggataaac
     4921 tgcaaaatgg ttctctgttg tcccgtatat cgcagaccat cctccttgaa aagggtcgat
     4981 cgactggtct atttaatatt aaccccgcct cttcaggcga atatttaaat tggcggcggg
     5041 ttaaatcccc gtccgcgaaa tggctcggtg gaaattgccg cgacgttaaa ttgaaaggac
     5101 aaagcaatgg ccaaaatcgg actcgcctgg gtcactcccg cacccctgac caaaccggaa
     5161 aatgtcgtca acttcgccaa aaagtgcgaa gcgatgggct gcgactcgat gtggaccatt
     5221 gaccgcatcg cctatgacaa tctcgaaccg ctgacgattc tcgccgccgc cgccggcgcg
     5281 acgcaaaaaa ttcgccttgg cacgtcagta ttactgggca acacgcgcca ccccgcccat
     5341 ctcgccaaga ttattgccac cttggatttt atctccaacg gccgcgtcac catcggctta
     5401 ggtttcggca gccgcgaacc ggactacaaa gcggtggaaa ttccgtacga acaccgcggc
     5461 tcccgggcgg tggagcaagt gcaactgatg aaacggctct ggaccgagga caatgtcaca
     5521 cacaaaggaa gattttataa cgtagaaaac ctgagcatcg ggccgcgtcc ggtacaaaaa
     5581 cctcacccgc cgctatggac cggcggcagt gccgaggtcg cgctcaaacg cgcaggcact
     5641 tgggccgacg gatttatctc cggcagcgcc gccatcgccg actttcacgt tacctgggac
     5701 aagatcgcca gctacgccgc cgccgccggg cgcaacccca atgacatcga aaaagccagc
     5761 ctggcattca tggcgatcga cgacgacaag gccaaggccg tgaaagtcat cgaagactac
     5821 accacgcgct actacggccg catcagaacc ccagtggaac cggtatctat tgtcggcaac
     5881 cctgaccaat gcgtcgagaa gattaattat ttcctgagca aaggtctcga cactctgatc
     5941 atcggcgtcg ccgatcccga tcccagtcag ttggatctgt ttggcgagaa ggtgctgtca
     6001 aagtttaaat cgtaataaaa ccaatccggc cgcgaaagcg ttccatggca atcatgcacg
     6061 cttgaactcg gttgagcctt gagattagtt tgagccaaga ggtgcacatg attaaggcgt
     6121 gggacgccga cgggcatgta tcggaatccg aagtcacgtt ctcggacaaa tactgggacc
     6181 ccaaactaaa ggatcgcaga ccgaccattc tagagaccgg tccggacggc ccgctttact
     6241 ggatgatcga tacccgctct tttcccgtgc gcagcggacc ctatcagcaa gccggctttc
     6301 cccttagcaa gaacggcgtc gcggcacgag tcactccggg aatcagtcct aacgatccgg
     6361 tcgaaagcgc ggaaatgcgc tcggtgaaag ctcgcctcga acaattggac cgggagaata
     6421 ccgcgcttca agtgatctat ccgacgatgt tcctgtccta tccgatcacc tatgatcggg
     6481 agttggcggt ggcaatgatg cgcgcctaca acagctgggt cgccgacctc gccagccaat
     6541 gtcccgagcg cttaaaatgg gtcacgatta tcgaccctat cgatccccag gcatcggtgc
     6601 gcgaaatcga gcgcggcaaa aaactcggag ccgtaggcgt aatgctcctc ggcgtcaccg
     6661 gcgacgtgcg ggtcgacgat ccgcccatgg aaccgatttg ggccgcagcc aacgacaccg
     6721 ggctccccgt cgcggtgcat accggacaca gctttcgcgc cctcggccag gttaacgaca
     6781 cgcaccatga caagacggcg ctatcttttt ggctgaccgt gcagttcgcc ttccatcggg
     6841 tgatttcgaa agggatcgcc gaccgctacc cgaacttgcg catcgccttt ctcgaagccg
     6901 gttgttcctg ggtgccggcg ttggtggagc ggatcgaaga atacagcggt ttccccggag
     6961 cgcgggcggg tgtcgatttt caccgcggtt ataccggcaa gcattggccc aaagaataca
     7021 tcgagcgcgg ccagatttac ttcggcttcg aagtcgacga gaaactgctg ccgttcgcca
     7081 tcgaagagtt cggcgatcag tgctgggtct acggctcgga tattccccac ggcgaccgcc
     7141 tctacggcgc ggtcgatgtc tttctcaagc gcgacgacat tagcgacgaa agcaagcgca
     7201 agctgcttgt cgataatacg gcgcgctttt atgggctcta ggggtggaaa cttgaggctg
     7261 tcctagtcta agacagacgg gcccgggcaa acagggatag caggagtatt ttgcaatgcg
     7321 ggccttcgca agatgagtag gacacgagtt catagcattg ccggacctga ctttaggttt
     7381 ttttctcagc caccggtgaa aagactttag cgcacgacga cgtgtacgcg ctgcacgccg
     7441 ttgttgccat agccgccgaa attccactgt tgcacggctg gctgcacgtt gccggtggca
     7501 tcgctggcgc gcacctgcag caggtgcgcg ccagctgtgg catgccattc gaatgtccaa
     7561 gatctccagg caaatgggga tgccgattcg ccgagttggg cttcggacca ggtcgcgccg
     7621 ccgtcgacgc tgacttcgac acgcgacacg ccgccttgcc ctgcccacgc tttgcccgtg
     7681 agcgtcacgg caccggcgtc gacgagccgg tcgcgggtca tgaaatccgg aatgccggga
     7741 ggaatcatca gggcgcgcgc tctgatcacg tcgaccacca ctccggggtc ctcggccgat
     7801 tgcgcgtatc ggtaggcgct taccatttga tagccttgaa acggctcggc gacggcttcg
     7861 attcggttca gccatttcac gctcgccatg ccgtaccagc ccggtttcac cagcctcagc
     7921 ggaaagccgt gctgcggcgg cagcggctcg ccgttcattt catagaccag caaaacctcc
     7981 gccctggtcg cttctgcgac ggtcagactg cgctggtagt gttgcacctg atcgccttcg
     8041 tagcctcgat ccaaaccggt aaaaacaatt tccgcggcag cgccgtcgat gccggcttcc
     8101 tgcaggatgg ttcgaagcgg cgttccggtc cactcggcgg tgccgatggc ttcgagcagc
     8161 cagggttggc tgatgcgccg cggcgcaaac aacgcccggc cgttgccggc gcattccatg
     8221 gtgaccggga tggtttggcg cggccgcttt ttaattgcct cgagagtcag ctcgaggggg
     8281 gtggcgactt ggccggcgat tttcaggcgc catccgtcgg cgtccaccgc cggtatgtcg
     8341 taatggatca gcagatagtg caggccggtt ggcgtgatcg gatagcgcag cgcctccagc
     8401 ggcatgccgc gattgcgcag cgcgagctgc agctcttctt tataaagcgc atcggtttga
     8461 gccaggccgc tgccgtcgcc aaccgttggg aaatatttgt ttgcgctgtt ttgttccatc
     8521 tgcgccaacc tctcgctaga ttttactttg ccgcgatcaa aatgaccggt atttaggcgc
     8581 ttgtgcggtg cgtcgggtcc agtttgtttt atgcaccgca atgcgttcat gagcttgtgc
     8641 tcgacgacag acaatgaaaa tggatgggat tctctccata gaaagtttgc tcgactcgtc
     8701 attcccgcgc acgcgggaat ccaggtttgg tctgcgccca attagcctgg atacccgctt
     8761 tcgcgggtat gacggaacgc cgctgacctc ttcattgtga gagaccatcg cctcacatgt
     8821 attttctaac gagagcgcga ggaacaccaa ggttgcggat gacgacacct ccgaa
//
LOCUS       DASQKS010000404         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1479484, whole genome shotgun sequence.
ACCESSION   DASQKS010000404 DASQKS010000000
VERSION     DASQKS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479484"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..250)
                     /locus_tag="VE170_10485"
     tRNA            complement(161..250)
                     /locus_tag="VE170_10485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ser,seq:tga)
     gene            623..826
                     /locus_tag="VE170_10490"
     CDS             623..826
                     /locus_tag="VE170_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYJ15906.1"
                     /translation="MATGTVKWFNATKGFGFIQPDSGGKDVFVHISAVEKAGLSSLNE
                     GAKVSYEEVANKGKTSAENLRVG"
     gene            925..1257
                     /locus_tag="VE170_10495"
     CDS             925..1257
                     /locus_tag="VE170_10495"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYJ15907.1"
                     /translation="MFIAMNRFQVIKGNETAFEQVWLSRDSHLGSVPGFVEFHLLRGP
                     EAEDHTLYASHTIWKNRDSFDAWTKSEAFRAAHANAGSNKPLYLGHPRLEGFETLQTV
                     TNSATHGG"
     gene            1951..2187
                     /locus_tag="VE170_10500"
     CDS             1951..2187
                     /locus_tag="VE170_10500"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ15908.1"
                     /translation="MSTGGLSMRCRLLNVSSQGAAIEVPNPALVPSRFQLMTEKDRVI
                     RFCRVIWIKQDRIGIAFDPPVADAGSGVPSRDLV"
     gene            2335..3249
                     /locus_tag="VE170_10505"
     CDS             2335..3249
                     /locus_tag="VE170_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYJ15909.1"
                     /translation="MGKPNSDRIGHAACAVAVAGACLMLANCGNLSRIDPKYGVSSSP
                     RVVASGEAVPKGGGTYRVGKPYTVAGRVYVPEENAKYRAEGLASWYGDNFHGRLTANG
                     EVFDMASLSAAHPTLPLPCYVRVTNLGNKKSLVVRVNDRGPYHGNRLIDVSNKAAELL
                     DFKQNGVASVRVEYVGRAPLEGSDDRQLMATLRTGVPAPSPSTVRIASARPFVPELAP
                     SERSIGDDVSTRRHTPDHTAMDIASINADSQISASSRVPARRQAMEEPREVSYGEGNV
                     AGHVRPVTAYAPVDQSGPSELLSGRGLY"
BASE COUNT          745 a          990 c          956 g          727 t
ORIGIN      
        1 agtaaatcga gcgccggaat aacgaacgtg ttcacgcaac gagccccttc acccaggctc
       61 tactatacag cttcaaggct atttcgttcc aacgaagcca atttacggta aaacatcaca
      121 caatccggtt ctggaaaatc cgagctggac agataaccat tggcggaagg ggtgggattc
      181 gaacccacgg tgggcttgca cccacgccgg ttttcaagac cggtgcctta aaccactcgg
      241 ccacccttcc aaccgatgga cactgtttag ctattgtcga agccgattgc ctaaaaggcc
      301 atctgctacc gctttgctac ccaacgcttt ggtgcgcttg tttatggcgg cacgtaacgc
      361 atcgtcaacc tgcgccacgg cgtcggcctg catattcggc aggacgtgcg aatacaatcc
      421 agcgtgatgc cgaccttgcc aagccggctc gctagcgatc tttgggtgaa agacgcaatt
      481 cgcctcttac agcatactgc aggcctgctt cgatggcgcc gagcacaaaa gtagcccgcc
      541 ggcgcgtgag cctgcggttt tccttgcgac cagcgaagct agcgctcatg atagttcgat
      601 cgttccaaaa gggagttcgg ccgtggcgac aggtacagtg aagtggttca atgcgacaaa
      661 gggtttcggg tttattcagc ccgacagtgg cggtaaggac gtgttcgtcc atatctcagc
      721 cgtggagaag gcgggcctta gctcgctcaa tgagggcgcc aaggtgagct acgaggaagt
      781 agcgaacaag ggaaagacct cagcagaaaa cctacgggtc ggctgatctt ctcgacccgc
      841 cggcttaccg gcgggtcatt tttcgtcgaa ggggaacgcg ttccacaatc ccgcgacggc
      901 acaaattacc aggagacttt tctgatgttc attgccatga atcgcttcca ggtcatcaag
      961 ggaaatgaaa cggcttttga acaggtctgg ctctcgcgtg acagccatct agggagtgtg
     1021 cctggattcg ttgaatttca tcttttgcgt ggacctgagg ctgaagatca cacactatat
     1081 gcctcgcaca cgatctggaa aaaccgcgac tccttcgatg catggaccaa atcagaggcg
     1141 ttccgagccg ctcacgcgaa tgctggcagc aataagccac tctatctggg tcatcctcga
     1201 ttggaggggt tcgaaacgct ccagaccgtg actaattctg caactcacgg tgggtaacga
     1261 ttggaggtca ttcagcgatt tgcgatcaag atcgatcccg agcgatcgtg cgcgcaagct
     1321 tagctgcttt taaggactta atcccagccc gccagttcat gccggcgggt tttcatatgc
     1381 ggggcggttc agcaaccgaa agttagaaag tcctaataaa taaggacatt tcataattga
     1441 acatacgcga cggacgacca ttagcgaatt tcaagaccgg tgccttaaac tactcggcca
     1501 ctcctccaac tcaggaattt ctaccgctta gcgcacccct gagcgcaatg cagacgcgct
     1561 gccgatccct tcaacaaagg tcagctgcat aagaaaactt tgcgaaatat actaaattgc
     1621 ggatattggt tctaagctgc tccccatgtt cctccagtac taccctaagt cgccgctgca
     1681 cgctttagag tgtcgcgatc cgcctccagt gccgtttccg cgaggagggc acctggcgcg
     1741 tcctgcacgg ggtgagatgc ggcccgaaca tgtcggaatt ttttgcgatt cgtgtttcat
     1801 taggctggcc gcatagaacc atcccgcacg ttcggatgac gatcataacg acgctgtaaa
     1861 accggacacg cgtgcggacg tacaggcaag aattcaccaa cagaagaccg ccgcaaacat
     1921 ccgcgcgtcg aaatcgacga gccggcttac atttccacgg gcggattaag tatgcggtgc
     1981 cggctgttga acgtatcttc acagggagcc gccattgagg tcccaaatcc ggcccttgtc
     2041 ccgtcgcgtt tccagctcat gaccgaaaag gatcgggtga ttcggttctg ccgggtcatc
     2101 tggatcaagc aggaccggat cggaattgct ttcgatccgc cagttgccga tgcaggctcc
     2161 ggcgttccga gtcgcgacct tgtttgacca cccgtctcgc tcctgaaggg ctcaccccat
     2221 ttccgccttg tttgctctgc gatatgttca cggcttggtg tggttatgga ccgtatcgtg
     2281 ctgaccggcg cggtgaagtg gggcagtcag ccggggaccg ggtcttgggg acggatgggg
     2341 aaaccaaatt cagatcgaat tgggcatgct gcatgcgctg tagccgtcgc gggcgcgtgc
     2401 ctgatgctgg ccaattgcgg taacctcagc cggatcgatc ctaagtacgg agtttcgagc
     2461 agccctcggg tggtcgcctc cggcgaggcg gtgccaaaag gcggcggcac ttaccgggtc
     2521 ggcaagccct atacagtggc cgggcgcgtt tacgttcccg aggaaaacgc caaatatcgc
     2581 gccgaagggc tcgcttcctg gtatggcgat aacttccatg gccgtctgac agccaacggc
     2641 gaggtttttg acatggcctc gctgtctgcg gcgcatccaa cccttccctt accgtgttat
     2701 gtccgagtga caaacctggg caacaaaaaa tccctggtgg tccgcgtcaa cgatcgcggg
     2761 ccgtatcacg gtaaccggct gatcgatgtc tcgaacaagg cggccgagtt gctggatttc
     2821 aagcaaaatg gtgtcgcgtc ggtgcgggtc gaatacgtcg gacgcgcgcc gcttgaaggt
     2881 tctgatgatc ggcaattaat ggcgaccctg cgaacgggcg tgcccgcgcc gtcgccgtcc
     2941 accgtcagga tcgcctcggc gcgaccgttt gtccccgaac tcgcgccgtc cgaacgctca
     3001 atcggcgacg atgtttcgac tcggcgccat acgcccgacc ataccgcgat ggacatcgca
     3061 tcgatcaacg cagattccca aatatctgcg tccagccgcg tcccggcccg tcggcaagcc
     3121 atggaagaac cgcgggaggt ttcatacggc gagggcaacg ttgcaggcca tgtccgcccc
     3181 gtcaccgctt atgcgccggt agaccagagc ggaccgagcg agctactctc gggccgaggg
     3241 ctctattaag cgtcgctcac ctaccgagaa agcttgaaat ctgctttggt ttattcggcc
     3301 cgcttctcag gcgcggcgcc atgttgtttg cgaccacccc aactgataaa aggatctatc
     3361 cggaatcgca ccatgacaac cagctcttcc acgcccgaca gacgcggggg attcgcct
//
LOCUS       DASQKS010000405         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1887492, whole genome shotgun sequence.
ACCESSION   DASQKS010000405 DASQKS010000000
VERSION     DASQKS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887492"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..1865)
                     /gene="glmM"
                     /locus_tag="VE170_10510"
     CDS             complement(426..1865)
                     /gene="glmM"
                     /locus_tag="VE170_10510"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYJ15910.1"
                     /translation="MIGNKKKLTRQLFGTDGVRGVANKDPMTSEMAIKLGRAIAKVLS
                     ESERDLPGRMPSPSFAHRNGETDKHRFKILIGKDTRLSGYMLETALASGIVSMGADVL
                     LVGPLPTPGVAFLTRSMRADAGVVISASHNPYQDNGIKFFSWDGFKLPDEVEARMEEM
                     IFSGETEESRPTASAIGKAARIQDAVGRYNVFLKNSYPRHLTLEGLKIVVDCGHGAAY
                     KVVPEVLTELGAEVLAIGVQPDGENINRRCGALYPETAREMLLHEGADFAVALDGDAD
                     RAVFVDETGEILDGDQVLAISALDMKEKGILKGNGVVGTVMTNLGLELAMHKAGLEVV
                     RAQVGDRYVVEKMIAGNFNLGGEQSGHILYLDHNSTGDGAITCLQMLALMVEKRKRLS
                     ELKNVMIRLPQVLVNVNVTERREFDQMIKVQQQIGEIERNLAGRGRVLVRYSGTELLA
                     RVMLEGEDAKKIRAMAQSIADEIRAEIGK"
     gene            complement(1889..2818)
                     /locus_tag="VE170_10515"
     CDS             complement(1889..2818)
                     /locus_tag="VE170_10515"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HYJ15911.1"
                     /translation="MKELWEKIVTLAGSNVGLKAVALTIAVGLWLAGHRDIERSIEVP
                     VEFRNIPSDLMVMDNRVDFVVLRVTGPRTLVSTMDSEEMKLALDLASAKPGSLSYPLS
                     GSLFNIPRGVTVARITPPVVHLRMEPVIKLTLPVSVRLSGKPAAGFRVAQITVNPERV
                     TVQGPAEEVRRLTAVETVPVDLNETRGVPKRRVRLSTDGKPLTISPDQVDIAVALQED
                     ESIRDFLQVEVNALDSKGLYTFTPRTVFLRVAGPKSIVDKLQLPADAVTFSLKGLSPG
                     EYTLPLVIKLPAGIKVIEQKPASVKVRVAKPTP"
     gene            complement(2815..3573)
                     /gene="cdaA"
                     /locus_tag="VE170_10520"
     CDS             complement(2815..3573)
                     /gene="cdaA"
                     /locus_tag="VE170_10520"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HYJ15912.1"
                     /translation="MLEVLHQIRWQDLLDIGIITFLVYRALQIVRGSRAMQMIIGLAV
                     ILVAYVSSRVLGLFTLNWILDNFLSSIILVIIVIFQSDIRRALTQVGSTPLFATLERS
                     QQRHEDVVEEVAQAAFALASKRIGALLVFQRDVGLSEFMEIGTRLDAWVSRELLESVF
                     QPHSPIHDGALVIQKSRVTAVRCLLPLSSNPHLRKSWGTRHRAALGVTEETDAVAVVV
                     SEQEGTVALVVGGNVTENIDGVRLRSALRDLIKS"
     gene            complement(3585..4508)
                     /gene="folP"
                     /locus_tag="VE170_10525"
     CDS             complement(3585..4508)
                     /gene="folP"
                     /locus_tag="VE170_10525"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYJ15913.1"
                     /translation="MSQSNPPDLNRTVSHFNPLVPMASDGSRELKTRHGRVDLARRTA
                     VMAVLNVTPDSFFDGGRHFDTGRAIADGLAMAQAGADIIDIGGESSRPAAVPVAEADE
                     LARVIPVIQGLRRQCTLPISIDSYKAAVARAALDAGADIVNDISALGFDPAMVQVVAS
                     EKVPVILMHMQGTPRTMQSDPHYDDVVREVRDFLAERLYDAMDAGIDAEAIVLDPGIG
                     FGKTIAHNLQLLRGLPVLAALGQPLLVGVSRKAFIGKILGLEPDQRLEGSLAAGVAAV
                     LGGASILRVHDVAPTCRAVRVADAIRFGFNG"
     gene            complement(4663..>5160)
                     /locus_tag="VE170_10530"
     CDS             complement(4663..>5160)
                     /locus_tag="VE170_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HYJ15914.1"
                     /translation="LTQQLPIDEKHTYGKEYLLNNLVILFGGRVAEELILDHTTTGAG
                     NDIEKATDLAHRMVCEWGMSEKLGPMTFGKKEEEIFLGRDFTQKVDYSESTAIEIDSE
                     VRRIIQESYHKAKDLLRTNIRLLHKVAESLLEKEVLDGSEIDAIVREFGGNGGAPYTP
                     SAAVA"
BASE COUNT         1095 a         1536 c         1472 g         1057 t
ORIGIN      
        1 ggatcgggaa gcttggccag ccgggcttgg cgcaccgtgg cgacatgatc gacgttgacg
       61 ccgagacgaa tcatggtatg cgaattttca ggggttggta ccgatagcca gtcatggtca
      121 acggctttct ctcactacga atgacctagc gcgccgacgc ggtaaccaaa aagaatggcc
      181 gcaaagaacg caaagagcac aaaataatag tggaatcgag agtcataagg gaagcagcgc
      241 aaatgatcac gcatcgttgt aggaagtgca aagagttcgg aatttgttga tgccttcagg
      301 ttcgtccgac tgccgagaat cacaagctca gccgtgcaac cccatggaag aaccaaagga
      361 ttcgcgtttt tttaagtact ttgcgttctt tgcggccagt cttctgaatc gcaatctcgc
      421 gatatttact tgccgatctc ggcgcgaatc tcgtcggcga tgctctgagc catggcgcgg
      481 attttttttg cgtcctcgcc ttccaacatc acccgggcca acagctcggt gccggaatag
      541 cgcaccagca cccggccgcg cccggcgagg ttgcgctcga tctcgccgat ttgttgctgc
      601 accttaatca tctggtcgaa ttctctgcgt tccgtcacgt taacattgac cagtacctgg
      661 ggcagccgga tcatgacgtt cttcaactca ctcagccgtt tacgcttttc caccatcagt
      721 gcgagcattt gcaaacaagt aatggcgccg tcgccggtgc tgttatggtc gagataaaga
      781 atatggccgg actgctcgcc accgagattg aaattaccgg cgatcatttt ttcgaccacg
      841 tagcgatcgc ccacttgagc gcgcacgact tcgaggccgg ccttgtgcat cgccagctcg
      901 aggcccaggt tggtcatcac cgtgccgacc acgccgttgc ctttaagaat gcctttctct
      961 ttcatgtcga gggccgagat cgccagcacc tggtcaccgt cgaggatctc gccggtttca
     1021 tcgacgaaca cggcacggtc ggcgtcgccg tccaaggcga cggcaaaatc ggcgccctcg
     1081 tgcagcaaca tttcgcgcgc ggtttcggga tagagcgcgc cgcaacgccg attgatgttc
     1141 tcgccgtcgg gctggacgcc gatggctagg acttcagcac ctaattcggt caacacttcg
     1201 gggacgacct tataagcggc gccgtggccg cagtcgacga cgatcttcaa cccttccagg
     1261 gtgagatggc gcggataact gttcttgaga aaaacattgt agcggccgac ggcgtcctga
     1321 atccgcgcag ctttaccgat tgcgctggcg gtgggccggg actcttcggt ctcgccacta
     1381 aaaatcattt cctccatgcg cgcttcgact tcgtcgggaa gcttaaagcc atcccaggaa
     1441 aaaaacttga tgccgttgtc ttgataagga ttatgcgatg ccgatatcac caccccggca
     1501 tcggcgcgca tgctgcgggt gagaaaagcc acccccggcg tcggtaacgg accgaccagc
     1561 aacacgtcgg cacccatgga aacgatgccc gaggccagag cggtctcgag catgtagccc
     1621 gaaagccggg tatccttgcc gatgagaatc ttgaagcgat gcttgtcggt ctcgccatta
     1681 cggtgcgcga acgaaggcga cggcatgcgg ccgggcagat cgcgctccga ttcagataag
     1741 accttggcga tggcgcggcc gagtttgatc gccatctccg aggtcatggg gtctttattg
     1801 gcgacgccgc gcacgccgtc ggtgccgaac agttggcgcg tcaatttttt cttattgccg
     1861 atcatcctgg ttgactcctg gttgcgattt acggcgtcgg cttggcgacg cgaaccttga
     1921 cgctcgccgg cttttgctcg attactttga tgcccgccgg caacttgata accaggggca
     1981 aggtatattc gccggggctc aaccccttca aactaaaagt aaccgcatct gcgggcaatt
     2041 gtagcttgtc gacgatactt ttgggaccgg cgacgcgcaa aaaaaccgtt ctcggcgtaa
     2101 aagtgtacag ccctttggag tccagggcgt ttacctcgac ctgcaggaag tcgcggatgg
     2161 actcgtcctc ctggagggcg acggcaatat ccacctggtc gggcgagatc gtcagcggct
     2221 tgccatcggt tgataaccgc actcggcgct tgggcacgcc gcgggtttcg ttgagatcca
     2281 ccggcacagt ttccaccgcc gtgagccggc gcacttcctc tgcgggcccc tgcactgtca
     2341 cccgttcagg gtttaccgtg atttgcgcga cgcggaatcc ggccgccggc ttgcccgata
     2401 agcgcaccga aaccggcagg gtcaacttga tcaccggctc catgcgcaga tggaccaccg
     2461 gcggggtaat ccgcgccact gtcacgccgc gcgggatatt gaataaactg ccactcaaag
     2521 gatagctgag agagccaggc ttggcactgg cgagatcgag cgccagtttc atttcctcgg
     2581 aatccatagt cgaaaccaac gtgcgcggtc cagtgacgcg caggacgaca aaatcgacac
     2641 gattatccat caccatgaga tcgctgggaa tattgcgaaa ctccaccggt acttcgatgg
     2701 agcgctcgat atcgcggtgg ccggcgagcc atagcccgac ggcgatggtc aaggccaccg
     2761 cctttaggcc aacgttgctg ccggccagcg tgacgatctt ttcccaaagc tctttcatga
     2821 cttgatcaaa tcccgcaatg ctgaacgcag cctgacacca tcgatatttt ccgtcacatt
     2881 gccgccgacc accagcgcca cggtaccttc ctgctccgac actacgacgg cgaccgcatc
     2941 agtctcctcg gtaacgccga gggcggcacg atggcgcgtt ccccaggatt tacgcaggtg
     3001 cggattggag ctgagcggca gcaaacagcg caccgcagtc acccggcttt tttgaatcac
     3061 cagggcgccg tcgtgaatcg gcgaatgggg ctgaaagaca ctttccaaca gctcacggct
     3121 gacccaagca tcgagccgcg tgccgatctc cataaattcg ctcaagccga catccctttg
     3181 aaataccaac aatgcgccaa tacgtttgct ggcgagggca aacgccgcct gggcgacttc
     3241 ttcgacgaca tcttcgtggc gttgctgcga tcgttccagc gtggcgaaca acggcgtgct
     3301 gccgacctgg gtgagcgcgc ggcgaatatc gctctggaag atcacgatga tcaccaagat
     3361 gatagagctc aagaagttat cgaggatcca gttcagcgtg aataacccga gaacgcggga
     3421 cgacacgtag gcgactagga tgaccgccag gccgatgatc atttgcattg cccggctgcc
     3481 gcgcacaatc tgcagcgccc ggtagaccag gaatgtgatg atgccgatat cgagcaggtc
     3541 ttgccagcgg atttggtgca gcacttctag cattgtcgcc tcggctaccc gttaaaacca
     3601 aatcgtatcg catcggcgac ccgcaccgcc cggcaagttg gcgcgacgtc gtggacgcgg
     3661 agaatactgg cgccgccgag caccgccgcc accccggcgg ccagactgcc ttcgagccgc
     3721 tggtccggct cgagaccgag aatcttgccg ataaaggctt tgcgcgagac gcccaccagc
     3781 aacggctgcc cgagggcggc gagcaccggc aagccgcgca gcagttgcag gttgtgcgcg
     3841 atggttttcc cgaaaccgat tccgggatcg aggacgatcg cttcagcgtc gatcccggca
     3901 tccatggcat cgtagagccg ctcggcgagg aagtctctca cctcgcgcac gacatcgtca
     3961 tagtgaggat cgctctgcat ggttcgcggc gtgccctgca tatgcattaa aatcaccggg
     4021 accttttcgc tggcaaccac ctgaaccata gccggatcga aacccagggc gctgatgtcg
     4081 ttgacgatgt cggcgccggc atcgagagca gcgcgagcca ccgcggcttt ataactgtcg
     4141 atcgagatcg gcagcgtaca ctgccggcgc aaaccttgga tcaccggaat cacgcgggcg
     4201 agctcgtcgg cttccgccac cggcacggcg gcggggcgcg aagactcgcc gccgatgtcg
     4261 ataatgtcgg cgccggcttg ggccatcgcc aaaccatcgg caatcgcccg gccggtgtcg
     4321 aagtgacggc caccgtcaaa aaacgaatcc ggcgtgacgt tgagcaccgc catcaccgcg
     4381 gtgcggcgcg ccaaatcgac ccggccatgg cgggttttca gctcgcggct gccatccgac
     4441 gccatcggca ccaacggatt gaaatggctt acggtgcggt tcagatctgg cggattgctt
     4501 tgggacatcg ctgcaaccga catgaaaaat aaactgaagt caaagaaaag ttaatgccac
     4561 agtctataaa taataacgcc ggctcggtcc attgccgacg ccggcgcata tagttaattg
     4621 ggcaggtgga gttggaaagc cgcagctcca ggaatgaacc tactaagcga ccgccgccga
     4681 cggcgtatat ggagctccac cattaccgcc gaattcgcgc acgatggcgt cgatttcgga
     4741 accgtcgagc acctcttttt cgagcaaaga ctcggcgact ttatggagca gccgaatgtt
     4801 cgtgcgcaag agatctttgg ccttatgata actttcctgg atgatgcgtc tcacttcaga
     4861 atcgatttcg atggcggtgc tttccgaata atcgactttt tgcgtgaagt cgcgccccag
     4921 gaaaatctct tcctcttttt taccgaaggt catcggaccg agcttctcgc tcatgcccca
     4981 ttcgcagacc atgcggtgcg ccaaatcggt ggctttctcg atatcgttac ccgcgccggt
     5041 ggtggtatga tcgaggatca gctcttcggc gacccggccg ccgaaaagaa tcactaggtt
     5101 attgagcaga tactctttgc cgtaggtatg tttctcgtcg atcggcaact gttgggtcaa
//
LOCUS       DASQKS010000406         6404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2747503, whole genome shotgun sequence.
ACCESSION   DASQKS010000406 DASQKS010000000
VERSION     DASQKS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747503"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /gene="mrdA"
                     /locus_tag="VE170_10535"
     CDS             complement(<1..1620)
                     /gene="mrdA"
                     /locus_tag="VE170_10535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYJ15915.1"
                     /translation="MTVLQINKQDGAPEIRRRANFLYGLLAIVFIGLVARLVHLQLVQ
                     GERYTFLSENNRVRLKRVAGTRGMIYDRHGELLVDSRPSFDLIFVPEDSKEPEQTLRV
                     LASRLGRDADELLQTYADSKSRSAFDEIVLGRDIEWAAVVAVETHQLDLPGVSLRIRP
                     RRNYVDGQTTAHILGYMGEINDRQLKNYKEQGYLLGDEIGQSGVERRWEEVLRGQHGG
                     QQVEVDALGRRVRVLHQVSDVPGYTVHLTLDRQMQQTAFEALKGKEGTIVAIDVNSGA
                     ILALASTPAFDPNVFARGIKGNEWNALITDRLRPLSNRATQGQFPPGSTFKIVMFIAG
                     LEEGAVDPNAQISDPGFFHFGNRNFRDWKQGGHGAVNLHYSLVISCDTYYYQLGNKLG
                     VDKIAKWARAFGLGEKTGIALDDERGGMIPDSAWKLKRYRQPWYPGETLSVAIGQGYV
                     TVTPLQMANMMAAVANGGKLYRPRLVSKVESLDGSNVREFAPELIRTIEMKPDTLARV
                     HNALADVVKGGTGGMARSPLIEVAGKTGTAQV"
     gene            complement(1628..2131)
                     /gene="mreD"
                     /locus_tag="VE170_10540"
     CDS             complement(1628..2131)
                     /gene="mreD"
                     /locus_tag="VE170_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYJ15916.1"
                     /translation="MKLFLLFLLVGIFLVVLQTTFFHLLPIGPVVPDLILVLCVYLGL
                     YHPSVGAALGSFLLGYSIDTVSSPILGFNAFAMSLVYLAVYLSARSIWLHHPIVSSVV
                     VLLAALVKGAAMVVIWAIFLSVDGFWAGAARYVLWEALLAALLAPLVFALLRRGQIFV
                     ERFNPTA"
     gene            complement(2159..2866)
                     /gene="mreC"
                     /locus_tag="VE170_10545"
     CDS             complement(2159..2866)
                     /gene="mreC"
                     /locus_tag="VE170_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYJ15917.1"
                     /translation="MWILRPLQIVGQGTANWIKGFRDNYDTLAGFRSENERLRNRLQT
                     LEIERQRLLEAEATNQRLQQLLDFRGELPAGAITASIIAASATSWFQSCVLNKGSADG
                     VRKDMAVVTPLGVIGKVVSVTGRTAKVLLVTDPNSGIDVMVQRTRSRGIVSGSLESGT
                     VLKYIKRSEDVREGDRLVTSGLDNIFPKGMLVGTVIKVRKQSRGLFQIVEVLPAIQAS
                     LVEEVLIVSGENEAAKK"
     gene            complement(3039..4073)
                     /locus_tag="VE170_10550"
     CDS             complement(3039..4073)
                     /locus_tag="VE170_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYJ15918.1"
                     /translation="MFDALFGVFSNDLAIDLGTANTLIYVKNQGIVCNEPSVVAVQQR
                     NERGGKKVLAVGAEAKRMLGRTPGSIVAIRPLKDGVIADFEITEAMLRYFIHKVHNRR
                     TLVRPRIIVGVPFGITEVEKRAVRESAESAGAREVYLIEEPMAAAIGAGLPITEPTGN
                     MIVDIGGGTTEVAVISLSGIVFSRSVRVGGDKMDEAIAQFIKRKYNLLVGERTAELIK
                     ITIGSAYPGDEVQTMEIKGRDLVAGVPKTVVITDEEIRDSLLEPINQIVEAVRLSLER
                     TPPELASDIVDRGIVLAGGGSLLRNLDILLREETGLPVMMADDPLTAVVMGAGKALDE
                     ITLLREVAVH"
     gene            4268..6178
                     /locus_tag="VE170_10555"
     CDS             4268..6178
                     /locus_tag="VE170_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HYJ15919.1"
                     /translation="MLDFLRKKKRNWAILFLLGLIIIVFVAFYGGNKMGDRPTSEVAM
                     INGEPISQREFAAEYQRTIERYREMLKGQLTEEMIKGLNLKGNIVENLVQKKLVLQEA
                     RSLGLVATDDDVVHQLAKVPEFQVAGRFNKERYLQLLQANRLQPAQFEEDQRDQITLQ
                     RLYNIIIDSVHVSDAEVHERYRLDQEKINLGFIKLSVNDFASQVKLTEDDIKKFYERN
                     KESLKEPLKIQVEYLTYPFEQFSADTQVSAKEIEEYYQNNQKTKFHRPREAKVRYISL
                     ALAPTASPEEKKVVLAKAEAIVKEARRGKDFAQLAKKESADATAAKGGDVGWVVQGQM
                     PPEVDKLIFGTGKGDVSDPVESPGGVQIFKIDDTREEKNLTLEEATAEITQILKTDQA
                     KKAAGKVADTDRVKAQAGTEFIKLAQASGVMAKETKLFAAGEILPEFGQNQEIYKIAF
                     GLGQQDVSQIVEANNGYIFLRLKQRKESTVPPLESIKEQVEKGAKEAKAYELTLQKGN
                     SLLDQLKKEKDLAKLAGANDLKVDETGWFQRNAPQLPKIGDLPELRGSGLPVSAQQPV
                     ADRLYTQKDAAYILAFKESRPADMEQFEKEKVLLKKQALVETRQRALAKYLESLKAKA
                     KIQFNNDFLEAS"
     gene            6196..>6404
                     /locus_tag="VE170_10560"
     CDS             6196..>6404
                     /locus_tag="VE170_10560"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYJ15920.1"
                     /translation="MQIVLVRHGATDWNLQHRCQGSSDRDLSEVGLRQAEEIAALLRP
                     ETLHGIYSSDLKRARRTAEVINAHH"
BASE COUNT         1618 a         1827 c         1693 g         1266 t
ORIGIN      
        1 cacctgggcg gtaccggtct tgccggctac ttcgatcagc ggcgaacgcg ccatgccgcc
       61 agtgccgcct ttaacgacat cggcgagcgc gttgtgcaca cgggccagcg tatccggttt
      121 catttcgata gtgcgaatca gctccggggc gaattcccgc acattagagc catcgagaga
      181 ttcgaccttg ctgaccaagc gcggccgata gagcttgccg ccgttggcta ctgcggccat
      241 catgttggcc atttgcaagg gcgtcaccgt cacataacct tggccgatgg cgaccgatag
      301 ggtttcgccg ggataccaag gctgccggta gcgtttcagt ttccaggcgg agtcgggaat
      361 catgccgccc cgttcatcgt ccaacgcgat gccggttttt tcaccgagac cgaaggcgcg
      421 cgcccacttg gcgatcttgt cgacgcccag cttattgccc aactggtaat agtaagtgtc
      481 gcaggagatc accagggaat aatgcaaatt taccgcgcca tggccgccct gtttccagtc
      541 acgaaaattg cgattaccga aatgaaagaa accaggatcg gaaatctgcg cgttgggatc
      601 gacggcgccc tcttcgaggc cggcaatgaa cataacgatt ttgaacgtcg atcctggcgg
      661 aaactgcccc tgggtggccc gattacttaa cggccgcaac cggtcggtaa tcagagcatt
      721 ccattcgttg cctttgatgc cgcgggcgaa aacgttggga tcgaaagccg gcgtgctggc
      781 gagggccaag atcgctcccg agttgacatc gatggcgacg atggtgccct cctttccctt
      841 gagcgcttca aaggcggtct gttgcatctg ccggtccaaa gtcaaatgca cggtgtagcc
      901 gggtacgtcg ctcacttggt gcagcacgcg cacccgccgg ccgagcgcgt cgacttccac
      961 ttgctgaccg ccatgctgcc cgcgcaatac ttcttcccag cgccgctcga ccccggactg
     1021 gccgatttcg tcgccgagaa gatatccctg ctctttgtaa ttctttaatt gacggtcgtt
     1081 gatctcgccc atgtaaccca ggatatgcgc cgtggtctgg ccatcgacgt agttgcggcg
     1141 cggacgaata cgcaaactta cgccgggcaa atcgagctgg tgggtctcca cggctaccac
     1201 ggcggcccat tcgatatccc ggcccaagac aatctcatcg aaggcagagc ggctcttgct
     1261 gtctgcgtag gtctgcaata gttcgtcggc gtcccgcccg agacggctgg ccagcacacg
     1321 caatgtctgc tcgggctcct tggagtcctc gggaacgaaa atcagatcaa aggacggccg
     1381 gctatcgact aacagctcac cgtggcgatc gtaaatcata ccgcgagtgc cggcaacgcg
     1441 cttgagccgg acgcggttat tttcggacag aaacgtgtag cgttcccctt ggaccaattg
     1501 caagtggacc aggcgggcga ccaagccgat gaagacgatg gcgagcagtc cataaagaaa
     1561 gttcgcccgc cggcggatct ccggcgcgcc gtcttgttta ttgatttgca gtactgtcat
     1621 tttttgatca agcggtagga ttgaatcgct cgacgaaaat ttggccacgc cgcaataggg
     1681 caaacaccaa tggcgcgagg agcgctgcca gcaaagcctc ccacagcacg taacgagccg
     1741 cgcccgccca gaagccatcg acgctgagaa agatcgccca gatcaccacc atcgccgcgc
     1801 ctttgaccag agcggcaagc aaaaccacca ctgaactgac gatcggatgg tgcaaccaga
     1861 tcgatcgcgc ggatagatag acagccaaat agaccaatga catggcaaag gcattaaacc
     1921 ccaatatcgg gcttgacacg gtatcgatcg aataacccaa tagaaacgat ccgagtgccg
     1981 ccccaaccga cggatgatag agccctaagt aaacgcatag gacgagaatt aagtcaggca
     2041 ccaccggacc gatcggcaac aggtgaaaaa atgtcgtctg taataccacc agaaaaatcc
     2101 cgacaagtaa aaacaacaag aacagtttca tgggcacata ggtcagagca ataatcggct
     2161 actttttcgc cgcttcgttt tcgccactta caatcaggac ttcttcgaca agcgaagcct
     2221 ggatagcagg caaaacctcg acgatttgaa acagcccccg gctctgtttg cgcactttga
     2281 tcacggtacc gaccagcatg cctttcggga aaatattatc caaacctgag gttaccaagc
     2341 ggtcgccttc gcgcacgtct tcgctgcgtt tgatatattt tagcacggta ccgctctcga
     2401 gcgaaccgga aacaatccca cgactgcgcg tgcgctgaac cattacatcg atgccgctgt
     2461 tcgggtccgt gaccaggagc acttttgccg tccgtcccgt gacgctaaca accttgccaa
     2521 tcacacccaa aggcgttacc accgccatat ctttacgcac tccatcggcg ctacctttgt
     2581 ttagaacgca gctttgaaac caactagtgg cgcttgcggc gataatcgat gcggtaatcg
     2641 caccggccgg taactcgccg cgaaaatcga ggagttgctg caagcgttgg ttggtcgctt
     2701 cggcttctaa cagccgttgc ctttcgattt ctagggtttg gagccggttt ctcagccgct
     2761 cgttttcgct gcgaaatccg gcgagggtat cgtagttatc gcgaaatccc ttgatccaat
     2821 tggcggtgcc ttgaccgacg atctgcaacg gccggagaat ccacatcaat agggcgccaa
     2881 ccggttcgtt tttgatctgg ccccgggccg tagcggttaa gatgtacagc gaaagcagta
     2941 aacagaaaca ggaacagaag aatatttggt tcctgcgtag gaaagcaagc atggcaggct
     3001 cgatcgtacc tgaaacacgg aatccatcca aacccggtct agtggaccgc aacctcccgc
     3061 aatagggtga tctcatccag ggccttaccg gcgcccatca ccaccgccgt aagcggatcg
     3121 tcggccatca tcaccggcag gccggtttct tcgcgaagaa gaatatccaa attgcgcagc
     3181 aacgacccgc cgccggcgag cacaatgccg cggtcgacaa tgtcggaggc aagttctggc
     3241 ggcgtccgct caagggacaa acgcaccgct tcgacgattt ggttgatcgg ttcgagcagc
     3301 gaatcacgga tctcttcgtc ggtaataacc acggttttgg gcacacccgc gaccaaatcg
     3361 cggcctttga tttccatagt ctgcacttca tcgccgggat aagccgaacc gatggtgatt
     3421 ttaatcagtt ctgccgtgcg ttcgccaacc aacaaattgt atttgcgttt gatgaactgg
     3481 gcgatcgctt catccatctt atcaccgcca acacgaaccg agcgggaaaa cacgataccg
     3541 gaaagcgaga tcaccgcaac ctcggtagta ccgccgccga tgtcgacaat catgttaccg
     3601 gtgggctcgg tgatcggcaa cccagcgccg atcgccgccg ccatcggttc ttcgatcaag
     3661 tagacttccc gcgcgccggc ggactctgcg gattcgcgca ccgcgcgttt ctcgacttcg
     3721 gtaatgccaa agggcacacc gacgatgatg cgcggccgca ccaaggtgcg gcgattgtga
     3781 actttgtgga tgaagtaacg cagcatcgcc tcggtgattt cgaagtcggc gatgacgcca
     3841 tccttaagtg gccgaatggc gacaatacta cccggagtac gtcccaacat gcgcttggct
     3901 tccgcgccca cggcaagaac tttcttgccg ccgcgttcat tgcgttgttg gaccgcgacc
     3961 acggaaggtt cgttacagac aatgccttgg ttctttacat aaattaaagt attggctgtc
     4021 ccaagatcga tggcaagatc attagagaag accccaaaaa gggcatcaaa catcagcaac
     4081 tccttcttgt gagcgcgcaa ccgactaaag ctatgagaaa atatcagatt ttgcgaaagc
     4141 tggcaaggta atgataaatc tctggaaaaa tcgttttaat cgaacgtttg taagttgcat
     4201 atgcgcagac cattttttat catgcttaca ctaatttcag gggtttttat gaaaggatcg
     4261 accaccgatg cttgattttc tccgtaaaaa aaaacgaaac tgggcgatcc ttttcctgct
     4321 cggcctcata atcattgttt tcgtggcgtt ttatggcggc aacaagatgg gcgaccgccc
     4381 cacctcggaa gtggcaatga tcaacggcga accgatcagc cagcgggagt ttgccgccga
     4441 atatcagcgc acgatcgagc gttatcgcga gatgctcaaa ggccagctca ccgaagaaat
     4501 gatcaaagga ctcaatctca aaggcaacat cgtcgaaaac ttggtgcaaa aaaagctcgt
     4561 cctgcaggaa gcgcgaagcc ttggcttggt ggcgacagac gatgacgtcg tccatcagct
     4621 ggccaaggtg ccggagttcc aggtcgccgg ccgcttcaac aaagaacgct atttgcagct
     4681 attgcaagcc aatcgtctgc aaccagcaca gttcgaagag gaccagcgcg atcagatcac
     4741 actgcagcgg ctctacaaca ttattattga ctcggttcac gtcagcgatg ccgaggtgca
     4801 tgagcgctat cgcctcgacc aagagaaaat caacctggga ttcattaaat tatcggtcaa
     4861 cgatttcgcc tctcaggtca aactcaccga ggacgacatc aagaaattct acgaacgcaa
     4921 caaagaatct ttgaaagaac cgctcaagat ccaagtcgaa tatctaacct accctttcga
     4981 gcaattctcc gccgacacgc aagttagcgc caaagagatc gaagaatatt atcagaacaa
     5041 tcaaaaaacc aaatttcacc ggccccggga ggccaaggtt cgctatatat ctctcgccct
     5101 cgcgcctacc gccagcccag aagaaaagaa agtggtatta gcgaaagccg aggctatcgt
     5161 caaagaagca cgccgcggca aagatttcgc ccagttggca aaaaaggagt ctgccgatgc
     5221 caccgcggcc aaaggtggag acgtcggctg ggtcgtccaa ggtcagatgc ccccggaggt
     5281 ggataaatta atttttggta ccggtaaggg cgatgttagc gatccggtgg aaagccccgg
     5341 cggcgtgcag atatttaaga tcgacgacac gcgtgaggaa aaaaatctta cattggagga
     5401 agccaccgcg gagatcacgc agatcctgaa gaccgatcag gccaagaaag cagccggcaa
     5461 ggtagccgat accgaccgcg tcaaagccca ggccggcacg gaatttatca aactggcgca
     5521 agcgagcggc gtcatggcca aagagaccaa attattcgcc gccggcgaaa tcctccccga
     5581 gttcggccaa aaccaggaga tctataaaat cgctttcggc cttggacaac aggacgtgag
     5641 ccagatcgtc gaagccaata acggctatat ttttctccgg ctcaaacaac gcaaggaatc
     5701 aaccgtaccg ccgctggaga gtataaaaga acaagtcgaa aaaggcgcca aggaagccaa
     5761 ggcctatgag ctgacgttgc aaaagggcaa tagcctgctc gaccagctga agaaggaaaa
     5821 agatctcgcc aagctcgccg gcgccaacga tttgaaagtt gacgagaccg gctggtttca
     5881 gcgcaacgcg ccgcagctgc caaaaatcgg cgatctcccc gagctgcgcg gcagcgggct
     5941 gccggtatcg gcgcaacagc cggtagccga tagactttac acgcaaaaag atgccgccta
     6001 catcttagcc ttcaaagaaa gccggcccgc cgacatggaa caattcgaaa aggagaaagt
     6061 gctgctgaag aaacaagccc tggtggaaac ccgccaacgg gctttggcca agtacctcga
     6121 aagtctcaag gccaaggcga agatccaatt caacaacgat ttcttggaag cgagctgagc
     6181 gtcgcggtac ccaacgtgca aatcgtcctc gtgcgccacg gcgctaccga ttggaatctg
     6241 caacaccgct gccaaggttc gagcgatcgc gacctgagcg aggttggcct gcgccaagcc
     6301 gaggagatcg ccgcgctact gcgcccggaa acccttcacg gtatctattc cagcgatctg
     6361 aaacgggcgc gacgcactgc tgaagtcatc aacgcgcatc acaa
//
LOCUS       DASQKS010000407         5874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3162534, whole genome shotgun sequence.
ACCESSION   DASQKS010000407 DASQKS010000000
VERSION     DASQKS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162534"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VE170_10565"
     CDS             <1..589
                     /locus_tag="VE170_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYJ15921.1"
                     /translation="RGAPGAAPGNAGPAAPGAGAPAAAQAAGGSPAAPAAGAPPAGGR
                     RGGGIGGPTGDGWSMEKEILEYVIPLVEKDYRVVKDKNARAIMGYSMGGGHATQIGFA
                     HPEVFSVVGGMSGGGANAITNSPERSATANKDYKIIFVGSGTEDGAINGARTSHQAMD
                     ALGIKHIFSEDVGYGHDYQVWRQYLYRLAQLTFRD"
     gene            646..807
                     /locus_tag="VE170_10570"
     CDS             646..807
                     /locus_tag="VE170_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15922.1"
                     /translation="MTDDVGRSLGVDGAGNVLVAGCFTDIVDFNPDPLATCYPGGRRN
                     GFLLRLGQS"
     gene            1037..1810
                     /locus_tag="VE170_10575"
     CDS             1037..1810
                     /locus_tag="VE170_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYJ15923.1"
                     /translation="MAPELEYALRLVVLFIAAVWAGAQNQIAGGGSFLMLPALMLTGI
                     DARAANITSTVALFPGQVTGGLMARHLAAGLERLSFRALTIISLIGGALGAVLLLLTP
                     STFFQQLVPWLVLFATLAFAWGSFGRKPVASSNHLNPWVAGAMQLAISIYGGYFGGGI
                     GFLMMAAFTLAGMAARTASATKNVLAAVMNATAVVVFLASGEVWWFAAAIACLGALVG
                     SVIGARLIERINERLLRVAIVVIGALLTIGLFLRSAHVI"
     gene            2170..3693
                     /locus_tag="VE170_10580"
     CDS             2170..3693
                     /locus_tag="VE170_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HYJ15924.1"
                     /translation="MSARIRSKLNQSQGQSLLDMPAARARAFLLKPESYCNIDLPNYF
                     VFSGVLSAVSKALQGTNLSTLRKETPRKSEDVNYAMLSNKDGRHAWRPFQLIHPALYV
                     SLVNLITEKNNWDTIRKRFSEFQGLGNFKCLSIPVRSLSRRKDKAAQILQWWQGIEQG
                     SIELALDYSYVLHADIADCYAAIYTHSISWALHGKSVAKEKRDDKSLVGNTIDAYIQD
                     MRHGQTNGIPQGSVLMDLISEIVLGYADLELSNRLTVKNIDEYRILRYRDDYRIFVNN
                     PQAGEAILKALTEVMIDLGLKLNTSKTTGSQSVVSSSLKPDKRAWFRGRQGDSNLQKH
                     LLVIHAHGADFSNAGSLTVALTSFHERLSKTRSVQNPISLISIAVDIAYQSPRTFPVC
                     AAIVSKLLSLLDSDEVRVQVIRKIYRKLSQLPNTGHMEVWLQRISHAYEPELEFNEPL
                     CKLVRGEVVCIWNNDWISSVKLKSALAPGKLVDRKKLGTIKPVILPEEVVLFSYERY"
     gene            3993..5453
                     /locus_tag="VE170_10585"
     CDS             3993..5453
                     /locus_tag="VE170_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HYJ15925.1"
                     /translation="MHENYRNGARTCVRSSGSNRRRVRQIGIAQSSVEILINLAPAAV
                     EKNGTWLDLPLAVIILQASGLLPDLAPEIESKLILFGELGIHGELRRVPGSLSLAFCA
                     KPGDTLIVPKGNEKECALILAKPGHEGCRVYPAGTLEEVLEFFHGRSKLPNALKEEIK
                     FQSIIDKALDFGRIRGQDEAKRAAVITAAGGHNLLLIGPPGEGKSLLASALPGILPQL
                     TDGEKVELTRIYSAVGALAEDGQAVTRRPMRSVHHSVSMQALIGGGSGVPRPGEITMA
                     HHGILFLDELPEFSRQALESVRQPLESGVVRITRVKASLEFPARFTLVAAMNPCPCGY
                     SGSAQCTCDSKSIEKYQKKLSGPLLDRIDLQVEIKPLTTEERFAPVQDGESPKLRALV
                     QKARQRQQQRFAGTDIPHNAAIPGGQVVDHCKFSEAGLRAFKEIASRGKVSTRTTDRL
                     AKVARTVADLDDSIEVQPHHIEEAAKFVAGGVLQSE"
     gene            5461..>5874
                     /locus_tag="VE170_10590"
     CDS             5461..>5874
                     /locus_tag="VE170_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15926.1"
                     /translation="MSTANDTELSICSFCEQVIQIKREHIGRRTGCPKCRRAILLFPN
                     KDLNIDDRLSSTWYYRRERLLLRAEDIGPISDEQFVELVRQGDIRVDTEICSQEITRS
                     QWVPAATVKLEVITERIDQRAAERQRRSKREIRKRE"
BASE COUNT         1368 a         1523 c         1646 g         1337 t
ORIGIN      
        1 tcgcggcgcc cccggcgccg ctcctgggaa tgcgggtccg gcagctccag gcgccggtgc
       61 gcctgcggcg gctcaagctg ctggtggatc acctgcggct ccagctgctg gtgcaccccc
      121 agccggtggc cggcgtggcg gcgggatcgg cggaccgacc ggcgacggct ggtccatgga
      181 gaaggagatc cttgaatacg tcattccctt ggtcgaaaaa gattatcgcg tggttaagga
      241 taagaacgcc cgcgctatca tgggctactc gatgggcggc ggccatgcca cccagatcgg
      301 ctttgcgcac cctgaagtgt tctcggttgt cggcggcatg agcggcggtg gtgccaatgc
      361 gatcaccaac agtccggagc ggtcggcgac ggcgaacaag gattacaaga taatcttcgt
      421 cggttcgggc accgaggacg gcgcgatcaa tggtgcccgc acatcgcacc aggcgatgga
      481 cgcgctaggc atcaagcaca tcttcagcga ggatgtcggc tacggccatg actaccaggt
      541 ctggcgccaa tacctttacc gattggcgca gctgaccttc cgcgattgag cgcgtaagtt
      601 tccctttttt ttcgtaaatc ggacgcaccc gtaagttaac gcggcgtgac cgacgatgtt
      661 ggccggagtc taggcgttga cggcgctggc aacgtcctcg tcgccggctg cttcaccgac
      721 atcgttgatt tcaaccccga cccactggcc acctgctacc ccggcggccg ccgcaacggc
      781 ttcttgctcc gtttgggtca gtcatgagga ggcactttcc ggccggcagt gctaggttca
      841 ggttgaaatg agcaagcaaa gcggggcaaa ggcttgaacg atctggtttg ggaaacggcg
      901 cgaaggcact taggtcaata aacgcaacgc caaagcgcac atgacgaacg gtcctatgtc
      961 acaaacggaa attccctggt attctccaca cgctttggtg aacgattgtc aggccgacta
     1021 acataagaat ttttgaatgg cgccagagct cgaatacgcg ttgcgcctgg tcgtcctatt
     1081 tatcgccgcc gtgtgggccg gcgcgcagaa ccaaattgcc ggcggcgggt cgtttctgat
     1141 gttgcctgca ctgatgctga ccggcatcga tgcgcgtgca gccaacatca cctcgacggt
     1201 cgcattgttt cccggtcaag tcaccggcgg cttaatggcg cgccacttgg ccgcaggttt
     1261 ggagcggctt tcattccgcg ccctgacgat catcagcctg atcggcggcg cactcggcgc
     1321 ggtactgctg cttctgacgc ccagcacttt cttccaacaa ttggtgccct ggttggtttt
     1381 gtttgccaca ctggcgtttg cctggggcag cttcgggcga aagccggtgg caagttcaaa
     1441 tcatctcaat ccatgggtag ccggagcgat gcagttggcc atctccattt acggaggtta
     1501 ttttggcggc gggattggct tcctcatgat ggctgctttt actctggccg gcatggcagc
     1561 gcggactgcg tccgcaacca agaatgtgct ggcggcggtg atgaacgcga ccgcagtcgt
     1621 agtattcctg gcctctggag aggtttggtg gtttgccgcg gctatcgcct gtctcggggc
     1681 gcttgtcggc agcgtgatcg gcgcccgcct aattgaacgt atcaatgagc gcctgctacg
     1741 cgtcgccatc gtggtgatcg gcgcgctact gaccatcggg ctgtttttac gatccgcgca
     1801 cgtgatctag gacttcgcga gcgacagcgg ggtcgagtta gaattcggcc cacgaaatag
     1861 tgcgaccgcg tggttcttgt cccttaatcc ggctatagca tggccgtaag gctgtccaaa
     1921 aaaaacattc gattttcgga tgctttgcat ttgtgtatgc ttatggggtt aataattcta
     1981 tattggtcag cacctcgaac caacttaagt gatgaggaaa acaaaaacca gagacaggaa
     2041 acatggcagc gcaatcatcg gtttcgaagc tgattgatgg cggacagccg acgcacttcg
     2101 ctataagatg gcagccgcgt ttgagtcgcg ggtgccggac gcggagcgga tcgtagggag
     2161 gtcgagactt tgagtgcgag aatccgcagt aagctaaacc aatcgcaggg acagtccctt
     2221 cttgacatgc cggccgcgcg tgcgagagcc tttctcctca agcctgagag ttattgcaac
     2281 atcgacctcc cgaattactt cgtcttcagt ggagtccttt cggccgtctc aaaggcactt
     2341 caaggaacga atctttcgac actgcggaaa gaaacgccgc gtaaatccga agatgttaac
     2401 tatgcgatgc tatccaacaa ggacggtcgc catgcttggc gacctttcca gctgattcac
     2461 ccagcccttt acgtctctct tgttaacctc attaccgaaa agaacaattg ggacacaata
     2521 cgaaaacgtt tctccgagtt tcaaggactc ggcaacttta aatgtttgag cattcctgtc
     2581 agatcactat ctcggagaaa ggataaggcg gcacaaattc ttcaatggtg gcaaggcatc
     2641 gagcaaggat caattgaatt ggcacttgat tactcctatg tgcttcatgc cgacatagcc
     2701 gactgctatg ccgcaatcta cacccattca atttcatggg ctcttcatgg aaaaagcgtg
     2761 gccaaggaga agcgggatga taagtctttg gtcggaaaca caattgatgc gtacatccaa
     2821 gacatgcggc atggccagac gaacggtata ccgcaaggct cggttttgat ggacctaatc
     2881 tccgaaatcg tgttaggcta cgcggacctt gaacttagta atcgcctcac ggtgaaaaat
     2941 atcgatgaat atcgcatcct tcgttatcgt gacgactacc gcatcttcgt aaacaatcct
     3001 caggctggcg aggcgatcct aaaggctctg acggaagtaa tgattgattt aggtctcaag
     3061 cttaatactt caaaaacgac tggcagccaa tcggttgtga gtagctcctt aaagccggac
     3121 aagcgagcgt ggtttcgagg gaggcaaggc gattcgaatt tgcaaaagca cctactagtg
     3181 attcatgctc acggcgctga tttctcgaat gcgggaagtc tgactgttgc attaacaagt
     3241 ttccacgaac gtctgagcaa aacgcgatcg gtccaaaatc ccatatcgct cataagtatc
     3301 gcggtagata ttgcatatca gagtccgcga acttttccgg tgtgtgcggc aatcgtaagc
     3361 aagctcttga gccttcttga cagtgacgaa gtaagagtgc aggtgattag aaagatctat
     3421 cgtaagctgt ctcagcttcc gaataccggg cacatggaag tgtggctaca acgaattagt
     3481 cacgcgtacg agccggaact ggaattcaat gagcccctgt gcaaactagt tcgcggcgaa
     3541 gttgtttgca tctggaataa tgactggata tcgtccgtca aactcaagtc ggctctggct
     3601 ccagggaaac ttgtagaccg aaagaaactt ggaaccatca agccggtgat actacctgag
     3661 gaagtcgtat tgttttcgta cgagaggtat tgacgagttg ggtcagcgtt gtgcctgccg
     3721 atttcgtcaa tgccagcaca caagtaaact ttatgcccgg gcatttcgct ttcattacgt
     3781 tttgtggcgg ttcagtatcg atatgttggt ttgatcttga gttactggta tcgtctaggg
     3841 gacaagctgg tgggcctttt caacttcaac gatcagctgc ttaggaggga tcacacgctt
     3901 cgaggaggaa ttctccatgg aattgacggt caattgatcg aggtgcaagc tcgcgccaca
     3961 agcgtacttc gacggccagt gccagcagtc caatgcacga gaattacagg aatggcgcgc
     4021 ggacctgtgt ccgaagctca ggatcgaatc ggcggcgcgt tcggcaaatt ggcatcgcgc
     4081 agagttcggt agaaatactt atcaatttgg cgccagctgc tgtcgagaaa aatggaactt
     4141 ggctggattt accactcgcg gtcatcatac ttcaggcttc aggcttgttg cctgacctgg
     4201 ccccagaaat tgaaagtaag ttaatcctat ttggggagct cggaatccat ggcgaattgc
     4261 gtcgcgtgcc cggttccctt tcactggctt tttgcgccaa acccggcgac acgctgatcg
     4321 tcccgaaagg aaacgagaaa gaatgcgccc ttattcttgc caagccaggg cacgagggat
     4381 gtcgagtata tccggccggc acattagaag aagtgctaga gtttttccac ggtcgcagca
     4441 aactacctaa cgcgcttaaa gaagaaatca agtttcagtc catcatcgac aaggcgcttg
     4501 attttggacg cattcgcggc caagacgaag ctaaacgcgc tgcggtgatt accgcggctg
     4561 gaggtcataa tctgcttctt attgggcctc caggagaagg gaagtccttg ttggcgagtg
     4621 cacttcctgg gattctgcca caacttaccg atggcgaaaa agtcgagctg acaagaattt
     4681 actctgctgt tggtgccctc gccgaagatg ggcaggccgt gacgcggcgc ccaatgcgat
     4741 cagtccacca tagcgtttcg atgcaagccc taattggtgg cgggtcaggc gtaccgaggc
     4801 caggcgaaat cacgatggcg catcacggaa tcttgtttct cgatgaactg cctgagttct
     4861 ctcggcaggc cttggaatca gtgcgccagc cacttgaaag cggagttgtc cgaattacgc
     4921 gtgtgaaggc aagtctggag ttcccagccc gctttaccct ggtagcggcg atgaacccct
     4981 gcccttgcgg ctattcaggc tcggcacaat gcacatgtga ttcaaagtca atcgaaaagt
     5041 atcagaaaaa actcagcggc ccgttacttg atcgaatcga tttacaagtc gaaataaaac
     5101 ctttgacaac cgaggagagg tttgcacctg tccaggacgg agaatcgcca aagctgcggg
     5161 ccctagttca gaaagctcgg cagcgccagc agcagcgatt tgccggaact gacatccccc
     5221 acaacgccgc aattcccggc ggccaggtag tggatcattg caagttttcg gaagcgggcc
     5281 tgcgtgcttt taaagaaatt gcatcgcgag gcaaagtctc gacgcgcacc accgatcgcc
     5341 tggcgaaggt cgctcggact gtggccgatc tagacgacag tatcgaagta cagccgcatc
     5401 acattgagga agctgccaag ttcgtggccg gcggcgttct ccaatcggaa tgagagttcg
     5461 atgagcacgg caaacgatac tgaattgtcc atttgctcat tttgtgaaca agtgattcag
     5521 atcaagcggg aacacattgg ccgccgcacc ggatgtccta agtgtcgtcg agcaatcctc
     5581 ctttttccaa ataaggattt gaatattgac gaccgtctgt cctcaacttg gtattatcgg
     5641 cgcgaacggc tgctgctaag agcggaagac attggtccga ttagtgatga gcaatttgtc
     5701 gaactggttc ggcaaggcga tatccgtgtg gacacagaga tttgcagcca agaaattaca
     5761 aggtctcagt gggtccctgc cgcaactgtc aaattggagg taatcaccga acgtatcgac
     5821 caacgcgcgg cagagagaca gcgacgttca aaacgagaaa tccgtaagcg agag
//
LOCUS       DASQKS010000408         5328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6113062, whole genome shotgun sequence.
ACCESSION   DASQKS010000408 DASQKS010000000
VERSION     DASQKS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6113062"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..718)
                     /locus_tag="VE170_10595"
     CDS             complement(32..718)
                     /locus_tag="VE170_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ15927.1"
                     /translation="MATTQTRRITRKELRQPDRFQVATEKALEYIQSHKSLACAAGAA
                     LVLAGLTILGWQFFKDRQNNAAALEFTDATALYQAEKYREALTALEKVQSYRWSRYAG
                     LAHLYQANSHIALGELDKALISGQHAVTATRPNTLYRQLALMTVANAAEQKNDCRQAI
                     ESYSEAQKIAAAQQGEALLGKARCLEKTGNAAGALAAYKEYVKDQPGSLISAKVSELE
                     SKVSAPAVVK"
     gene            complement(757..1284)
                     /locus_tag="VE170_10600"
     CDS             complement(757..1284)
                     /locus_tag="VE170_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HYJ15928.1"
                     /translation="MSNKSECIEMSVGGLTLDPVTKTPIVILKDTENKLNLPIWIGLL
                     EATAMATEIEGITMARPMTHDLLKTLLGEVGCSVQSIEITELKENTYYAAINLTLAGG
                     RPLMLDSRPSDAIALALRAKCPIYVAKAVLEASSVLQQDETKEMPVENVSNVSQEKWG
                     EILEKMTPDDFKYKQ"
     gene            complement(1281..2648)
                     /gene="miaB"
                     /locus_tag="VE170_10605"
     CDS             complement(1281..2648)
                     /gene="miaB"
                     /locus_tag="VE170_10605"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYJ15929.1"
                     /translation="MAKKLFIQTYGCQMNQYDSERIAQVLARKDYVQTDRIDSADVLL
                     LNTCSVRDRAEQKVYSALGSWKEFKDYREGVIIGVGGCVAQQEGEKLLRRVPHLDLVF
                     GTHNIHKLPEMIEQVENARERPVETAFYRDAAYMDGGVLRTHVDGVKAFVTIMQGCNK
                     VCSFCIVPHVRGREVSRPSDKILGEIESLARHGVKEVMLLGQNVNSYGKLTPGELSFA
                     ELLAEVDRIDGLRRIRFTTSHPQDLSPELIEGFATLQNLCEHLHLPVQSGADTVLARM
                     RRGYAKAEYLDRLARLRDRRPDVALSTDIIVGFPGETDGEFEETLELLRLVEYDDIYS
                     FTYSPRPQTVSAKIYPDDVHDDVKKERLKIVQTLQREISLAKNRQRIGDREEVLVDGP
                     SKLRAEQIMGRTRSNRIVNLTGSSDLIGEFVHARIIGATANSLLGELMCANAAIESQR
                     EEGRP"
     gene            complement(2653..4059)
                     /locus_tag="VE170_10610"
     CDS             complement(2653..4059)
                     /locus_tag="VE170_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ15930.1"
                     /translation="MQARVIEFANVLRRNGIRVSLSENMDAFRALELIGIADPQLFRN
                     ALRTTLIKRSGDQKSFDELFDYFFLGIGQALDAIDRKIMEELGLSPEKFQEMLEQIQK
                     LMKEMEGDLAELTKALLNNNRAELERLLREAMQQETQEGTPDSLRHTPYTRMMMRLGL
                     DRVQSEIERFRGMLQMLGENGEDLQNVLRYLDERMRDLNRLLREVIQQEQRKQGAELR
                     DSSQRGALADKSFSFYTEDDIRRMNDAVARLAQRLKNRLSVRRKKAARGRFDVKATLR
                     KNMQYGGVPFLIQLDRRKKTKPQVMVLCDISDSVLNASRFMLQFVYSIQDLYAKVRSF
                     VFVSEIGEVTKLFEEHDIQTAVETALKGDVIDVFSHSNFGRAFEQFHRNFFASVTSKT
                     TVLVIGDGRNNYNRPNEWVLREVKQKAKQLIWLNPESRMTWGIGDSEMPRYAPHCHIA
                     EECRSINQLYKIVDLIVP"
     gene            complement(4089..5045)
                     /locus_tag="VE170_10615"
     CDS             complement(4089..5045)
                     /locus_tag="VE170_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYJ15931.1"
                     /translation="MFQSVDEVIAGLGKQKYLCNKNVATVVYLGTSLQKPLLVEGPAG
                     VGKTELGKVLADSLGMELIRLQCYEGLDEAKALYEWEYAKQLLYTQILKDKIGEILEG
                     AKTLQQAVDHVANQDGVFYSDRFLLPRPLLRAVLSEKPVVLLIDEIDKSDAEFEAFLL
                     EVLSDFQITVPEIGTLKAKHIPLVVLTSNNSREVSDALKRRCLHLYLDFPDPEREKEI
                     IKLKVPNVGDKLADEVVRLLHRLRKLDLKKTPSISESLDWVRALTLLNIKDLDNELVE
                     QTMSALMKYEADIRKAHQELKSYLAEKQARRPDVSDDKDHLH"
BASE COUNT         1080 a         1510 c         1467 g         1271 t
ORIGIN      
        1 aatatatctt atcagactct aattaagggc tctatttaac caccgcaggc gccgaaacct
       61 tgctttcaag ctcggagact tttgcgctta tcagcgatcc cggctggtct ttgacgtatt
      121 ccttgtaggc ggccaaagcg ccggcagcat taccggtctt ttcaaggcaa cgtgctttac
      181 cgagcaacgc ttcgccttgc tgtgccgcgg cgattttctg tgcttcgctg taagattcaa
      241 tcgcttggcg acagtcgttt ttttgctccg cggcgttagc cacggtcatc aaagccaact
      301 gacggtacaa cgtgttgggc cgggtcgctg taaccgcatg ctggcccgag atcaacgcct
      361 tatccagctc gcctaatgcg atatggctat tggcctgata cagatgcgcc aagccggcat
      421 aacgagacca gcggtacgac tgaacctttt ccaatgctgt cagggcctcg cgatacttct
      481 cggcctgata aagcgccgtg gcatcggtga actccagggc tgccgcatta ttttgcctat
      541 ccttgaagaa ctgccatccc aggatggtga gaccggccag aacgagcgcg gcgcccgcgg
      601 cacaggctaa actcttgtgg gattggatat actccagcgc cttttcggtg gccacttgaa
      661 accgatcagg ctgacgcagt tctttacggg ttattcgtcg cgtttgtgtc gtggccatgg
      721 attgaatttg ggattcgaaa taatcacttc aactagctat tgcttgtact tgaaatcgtc
      781 cggggtcatt ttctcgagga tctcgcccca tttttcctgg gatacattgg agacgttctc
      841 aaccggcatc tctttggttt cgtcttgttg caggacgctc gatgcttcca acactgcttt
      901 ggcgacatag atcggacact tggcccgcag cgccaatgca atggcatcgc tggggcggga
      961 gtcgagcatg agtggccgcc cgccggccaa agttagatta atcgccgcgt aataagtgtt
     1021 ttctttaagt tcggtgatct cgatggactg cactgagcag ccgacttcac ccaataaggt
     1081 cttgagcaaa tcgtgagtca tcggccgggc catggtaatg ccttcgatct cggtggccat
     1141 ggcggtcgct tccagaaggc cgatccagat cggcagattc agtttgttct ccgtgtcttt
     1201 gaggatcacg atgggtgtct tggtcaccgg gtcgagagtt aaaccgccga ccgacatctc
     1261 tatgcattct gatttgttgc tcatggtcta ccctcttctc gctgggattc tattgcagca
     1321 ttagcgcaca tcaactcgcc gagcaatgag ttggcggtgg cgccgataat tcgagcatgc
     1381 acgaattcac cgattaagtc ggaggaaccg gtgagattaa cgatgcggtt gctacgggtg
     1441 cgccccatga tttgttcggc cctaagtttc gacggtccat cgaccaggac ctcttcgcgg
     1501 tcgccgatgc gttggcggtt tttcgccagc gatatttcgc gctgcaaagt ttgcactatc
     1561 tttagacgct cttttttcac gtcgtcatga acatcgtcgg gataaatttt cgcggagacg
     1621 gtttgcggcc gtggcgaata ggtgaacgag taaatatcat cgtactccac caaacggagt
     1681 aattctagcg tctcttcgaa ttcgccatcg gtttcgccgg gaaagccgac aatgatgtcc
     1741 gtgctgagcg ccacatcggg acgacggtcg cgcagacgag cgagccggtc caaatactcc
     1801 gccttggcgt agccgcggcg catccgagcc aacaccgtgt ccgcgcccga ctgaacaggc
     1861 agatgcaggt gctcgcacaa attttgcagc gtagcgaacc cttcgatcag ctccggcgac
     1921 aggtcttgcg gatgggaagt ggtaaatcga atgcgccgca agccatcgat ccggtcgacc
     1981 tcggcgagaa gctcggcaaa gctcaactca cccggcgtga gtttgccgta ggaattgacg
     2041 ttttggccga gtaacatcac ttccttgacg ccgtggcgcg ccagcgactc gatttcgccg
     2101 agaattttat cgctgggccg gctcacctcg cggccgcgca catggggcac gatacagaag
     2161 ctgcacactt tgttgcagcc ctgcatgatc gtcacaaagg ccttaacgcc gtcgacatgc
     2221 gtgcgcagca cgccgccgtc catgtaagcc gcatcgcggt aaaaagcggt ctccaccggc
     2281 cgttcccggg cgttttccac ctgttcgatc atttccggca gcttgtgaat gttgtgggtg
     2341 ccgaaaacca aatccagatg ggggacgcgc cttaacagct tttccccttc ctgttgtgcc
     2401 acgcaaccgc cgacgccgat gatgacgcct tcgcggtagt ctttgaattc cttccagctg
     2461 cccaaagcgc tatagacttt ctgctccgcc cgatcacgca cgctgcaagt attgagcaga
     2521 aggacgtccg cgctatcgat gcggtcggtc tggacataat ccttgcgcgc cagtacctga
     2581 gcgattcttt ccgaatcgta ctggttcatc tggcagccgt aggtctgaat gaaaagcttt
     2641 ttcgccataa ccttagggca caatcagatc gacgatctta taaagttgat tgatgctgcg
     2701 gcactcctcg gcgatatggc aatggggcgc gtagcgcggc atctcgctgt cgccgatgcc
     2761 ccaggtcatg cggctttccg ggttcagcca gatcaactgc ttggcctttt gtttgacttc
     2821 gcgcagcacc cattcgttcg ggcggttata attgttgcgc ccatcgccga tcaccaagac
     2881 ggtggtcttg ctcgtgaccg aggcaaagaa gttgcgatgg aactgctcga aggcgcggcc
     2941 gaaattcgaa tgggagaaga cgtcgatgac atcccctttc aacgccgtct cgacggcggt
     3001 ctgaatgtca tgttcttcga atagcttggt gacctcgccg atctcggaga cgaagacaaa
     3061 actgcgcacc ttggcgtaga gatcctggat agaataaacg aactgcaaca tgaagcgtga
     3121 agcgttcaac accgagtcgg agatatcaca tagcaccatc acttgcggct tggtcttctt
     3181 gcgccggtcg agttgaatca gaaacggcac gccgccgtat tgcatgtttt tacgcaaggt
     3241 ggctttaacg tcgaaacggc cacgggcggc ttttttgcgc cgcaccgaca ggcggttctt
     3301 caagcgctgc gccagtcgcg ccacggcgtc gttcatgcgc cggatatcgt cttcggtgta
     3361 gaaagaaaaa ctcttgtccg ccagggcgcc gcgctgggag gaatcacgca gttccgcgcc
     3421 ctgtttgcgc tgctcctgct ggatcacctc gcgcaacagc cgattcaagt cgcgcatgcg
     3481 ctcgtctaaa tagcgcaaga cgttttgcag atcttcgcca ttctcgccga gcatttgcag
     3541 catgccgcgg aagcgctcga tttcgctctg cacgcggtcg aggcccaagc gcatcatcat
     3601 gcgtgtgtag ggcgtgtggc gcagactgtc cggcgtaccc tcctgggtct cctgctgcat
     3661 cgcttcgcgg agcagacgct cgagctcggc gcggttattg ttgaggagcg ccttggtcag
     3721 ctcggccagg tctccttcca tctccttcat gagtttctgg atctgctcga gcatctcctg
     3781 aaacttttcc ggcgacagcc ccagctcctc catgatcttg cgatcgatgg cgtcgagggc
     3841 ttggccgatg cccaagaaaa agtaatcgaa tagttcgtcg aaggatttct ggtcgccgct
     3901 gcgcttgatc agcgtcgtgc gcaaggcatt gcgaaacagt tgcgggtcgg cgatgccgat
     3961 cagctccaga gcgcggaacg catccatgtt ttccgacaac gacacccgga tgccgttgcg
     4021 gcgcaataca ttggcgaact cgatgacccg cgcttgcata gtgttgctgc aaccccgcgt
     4081 cctagcggct aatgtagatg atccttgtca tcgctcacgt cgggacgccg ggcttgcttc
     4141 tcggctagat aactcttgag ttcctgatgc gccttgcgta tgtcggcttc gtacttcatc
     4201 agcgccgaca tggtctgctc gaccagttca ttgtcgaggt ctttgatatt gagcaatgtc
     4261 aatgcacgca cccagtccag gctctcgctg atgctcgggg tctttttcag atccagcttg
     4321 cgcaagcggt gcagcaagcg caccacttca tcggcgagct tgtcgccgac attgggtact
     4381 ttgagcttga tgatctcttt ctcacgctcg ggatcgggga agtcgaggta caggtggagg
     4441 cagcggcgct tgagcgcgtc ggaaacttcc cggctgttat tgctggtcag caccacgagc
     4501 gggatatgct tggccttgag cgtaccgatc tcgggcacgg tgatttgaaa gtcgctcaat
     4561 acttcaagca aaaacgcctc gaactcggcg tccgatttat cgatctcatc gatcaacagc
     4621 accaccggct tttccgacaa gaccgcgcgc agcaacggcc gcggcaggag aaagcggtcg
     4681 gagtaaaaca ccccatcctg gttagcgaca tggtcgaccg cttgctgtag ggttttggcg
     4741 ccctccagga tctctccgat tttgtccttc aaaatttgcg tgtagagcag ctgcttagca
     4801 tactcccatt catataaggc tttcgcttcg tcgagccctt cgtaacactg gaggcgaatc
     4861 agctccatgc cgagagagtc cgccagcacc ttgcccagtt cggtcttccc cactcccgcg
     4921 gggccttcca ctaacagcgg tttttgcagc gacgtgccca ggtagaccac ggtagcgacg
     4981 ttcttgttgc acagatattt ctgtttgccc aggccggcga tgacttcatc aacagattga
     5041 aacatggttg ccctttccga atttatcgaa gctaacacaa tcaatattca acaacaatca
     5101 aggcttagcg gcggcgacgt aggaaccaaa gatccctcgc aaaggcgcaa agacggcagt
     5161 tcggagctcg attaattctc aagctttacg ccgtcgcgcc tcggcgggag tgaagcgaga
     5221 ggcacccgtg cggggcgagg cagacataat ctttaggctg ggggtatcct tatcttgcac
     5281 aggggatcgc acgaagattg cacaagggcc ggccattcgg taagtctt
//
LOCUS       DASQKS010000409         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2338297, whole genome shotgun sequence.
ACCESSION   DASQKS010000409 DASQKS010000000
VERSION     DASQKS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338297"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VE170_10620"
     CDS             complement(<1..613)
                     /locus_tag="VE170_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ15932.1"
                     /translation="MEPITTEILVLGAGPGGYAAAFYAADKGKQVTLVEQNPRLGGIC
                     LNVGCIPSKALLHATEILRESKASAARGIVLGAPKIDLEQLRRWKTGILDKLGQGIKT
                     LAEHRKVQVIHGRGHFEDSQTLRVETSKGQKFVRYEKAIIAVGSKPALPNAFDLGNPR
                     VMTSTGALEIPDIPENLLVVGGGYIGMELGTVYAALGSNVVVLE"
     gene            complement(626..1951)
                     /locus_tag="VE170_10625"
     CDS             complement(626..1951)
                     /locus_tag="VE170_10625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYJ15933.1"
                     /translation="MDVKLPQLAEGVEGGTVVSIMVTEGQAIEKDQPFMELETQKAVG
                     SIPAPSAGVVTKIHVKQGMEVNVGQTLISIEAAASDTPAAAPAVQAAPSAAANPNQPP
                     TPTGEQSHSSAQAQHAEPVDDYRYQSASGMPPPASPAIRKIARDLDLDLTRVRGSESG
                     GRINMADLRAYVRKLQDLAVQNPAPAASVAVPSSASRPTEPIDFAKWGPIRREKMSPL
                     RRTVSRRMVESWTTIPKINQFADADITALLALRKKHSPVYEKRDTHLTLTSFLLFILG
                     RALKKHPRANASLDEASLEIVYKDYCHIGVAVDSEGGLIVPVLRDADKKNLFELAVDL
                     LALTEKSRQRKISIEELQGGSFTISNQGSLGGGYFTPIIYAPQVAILGVGQGQAKPVA
                     VDGKIAIRTILPLCLAYDHRVLDGADAVRFLKDIIAGLEAFAEADIKLK"
     gene            complement(1987..>4587)
                     /gene="aceE"
                     /locus_tag="VE170_10630"
     CDS             complement(1987..>4587)
                     /gene="aceE"
                     /locus_tag="VE170_10630"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYJ15934.1"
                     /translation="SILKNEQPEQAALLVEELSDRLRQSGLKIPLAVSTPYINTIPPE
                     KQPPYPGNREIERRIKSYVRWNAMAMVVKANRLHADIGGHISTYASSATLYEVGQNHF
                     FRGSDGTHLGDMVYFQGHASPGVYSRAYVERRIDAPKLHHFRQELSSVGGTSSYPHPY
                     LMPDFWQFPTVSMGLAPIMSIYQARFVRYMKSRGLISGEEPRIFCFVGDGEMDEPESS
                     GALTLASRENLDNLVWVVNCNLQRLDGPVRGNGKIIQELEALFRGAGWNVIKVIWGSD
                     WDPLLAMDHDGLLLKRMEEAVDGDYQKYSVEPGSYTRKHFFGKYPALLEMVNHMSDDQ
                     IHKLLRGGHDPMKVYSAYLAAVQHKGAPTVILAKTVKGYGLGEAGEGRNITHQQKKLN
                     ERELREFRTRFDIPLPDEEVVETPFYRPPIDSPETEYLLERRKQLNGFLPERKVRAQP
                     LNVPADSEYAEFYKGSGGHSMSTTMAAVRLLTKLLRHEGIGKRIVPIIPDEARTFGMD
                     SLFRQIGIYSSKGQLYEPVDRETLLYYNETKDGQILEEGITEAGAMSSFIAAGAAYAN
                     LGVNMVPFYFYYSMFGFQRIGDLIWAAADNKSKGFLLGATAGRTTLNGEGLQHQDGHS
                     HILASTVPTLLTYDAAFAYEVAVIVKDGLRRLYTEGEESLYYLTLYNESYEMPAMPEG
                     AEEGILKGLYKVKAATKGKKYKAHLFGSGPILREALRAQEVLAEKFDVSADVWSATSY
                     KLLRGDALNTKRWNMLHPTAVPKKSYLESILENEQGVFIAVSDYMKMVPDQIAPWVPG
                     GLMSLGTDGFGRSDTRANLRRFFEVDAELTTLATLYALHQKEAFPAQVVEAAIRTLGI
                     DPEKSFPFYR"
BASE COUNT          909 a         1261 c         1352 g         1065 t
ORIGIN      
        1 cttccagcac gactacgttg ctgccaaggg ccgcatagac cgtgccgagt tccatgccga
       61 tatagccgcc gccgacgacc agcagatttt ccggaatatc gggtatctct agcgctccgg
      121 tggatgtcat tactctagga ttgcctagat cgaaagcgtt gggcagggca ggcttggagc
      181 cgacggcgat gatcgccttt tcgtaacgga caaacttttg ccctttggaa gtttcgaccc
      241 tgagcgtttg tgaatcctcg aagtggccgc ggccatggat gacctggacc ttgcgatgtt
      301 cggcgagggt cttgatccct tggccaagct tgtcgagaat gccagttttc caacgacgca
      361 gctgttcgag atcgatcttc ggcgcgccaa ggacgatgcc gcgggcggcc gatgctttgc
      421 tctcgcggag gatttctgtg gcgtggagta aggccttcga cggaatgcag ccgacgttga
      481 ggcagatacc gccgaggcgt ggattttgct cgaccagagt gacctgcttg cctttatccg
      541 ccgcataaaa ggccgcggcg tagccgcctg gaccggcgcc cagaactaag atttccgtcg
      601 taatcggttc cataattgaa tctaactact ttagcttgat gtcggcttcc gcgaaggctt
      661 cgaggccggc gatgatgtct ttaagaaagc gcacggcgtc ggcgccgtcg agcacgcgat
      721 gatcgtaggc taaacaaagc ggcaggatgg tcctgatggc gatcttgccg tcgacggcga
      781 ccggcttagc ttggccttgg ccgactccca aaatcgccac ctgtggcgca taaatgatcg
      841 gcgtaaagta gccgccgccg aggctccctt ggttggagat cgtaaagctg ccgccttgca
      901 gttcttcgat ggaaatcttg cgctgccggc ttttctccgt cagcgcgagc aagtccacgg
      961 cgagctcgaa caagtttttt ttgtcggcat cgcgcagcac cggcacgatc agaccgcctt
     1021 cgctgtcgac cgccacgccg atatggcaat agtccttgta gacgatttcc agcgatgctt
     1081 cgtccaaact ggcgttggcg cgcggatgtt tttttagcgc gcgtccgagg ataaatagga
     1141 gaaatgaagt gagcgtgaga tgggtgtcgc gcttctcata aactggcgag tgtttcttgc
     1201 gcagggcgag cagcgctgtg atatcggcgt cggcaaactg attgattttt ggaatcgtgg
     1261 tccaagactc caccatgcgc cggctcaccg tgcgccgtag cggcgacatc ttttcgcggc
     1321 ggatcggccc ccatttggcg aagtcgattg gctccgtcgg ccttgatgcg gatgatggca
     1381 cggccaccga agctgccggc gctgggtttt gcacggcgag atcctggagc tttcgaacat
     1441 aggctcttag atcggccata ttgatgcgcc cgcctgattc gctaccgcga accctggtca
     1501 ggtcgaggtc gaggtcgcgg gcgattttgc ggatcgccgg cgaggcgggc ggcggcatgc
     1561 cgctagccga ttggtaacgg tagtcatcaa ctggctcggc gtgttgcgct tgagccgagc
     1621 tgtgagattg ttcgccggtt ggcgtaggtg gctggttcgg gttcgcggct gcgcttggag
     1681 cggcttgcac cgccggagct gcagccggag tatcgctggc tgccgcctcg atggaaatga
     1741 gcgtctggcc aacattgact tccatgcctt gcttgacatg aatcttagtg accacgccgg
     1801 cactgggggc ggggatggaa cccacggctt tttgcgtttc aagttccatg aaaggctggt
     1861 ctttttcgat cgcctgccct tccgtgacca tgatgctgac gaccgtgccg ccctctacgc
     1921 cttcagcgag ttggggaagt tttacgtcca tggagaaaat tcgctgctgt cgagtgctta
     1981 gattgcttat cgatagaacg ggaatgattt ttccggatcg atgccgaggg tgcggatcgc
     2041 tgcctccacg acttgcgccg gaaacgcctc tttctggtgc aatgcgtaga gtgtcgcaag
     2101 ggtcgtcagc tcggcgtcca cctcgaaaaa tcgtcggagg tttgcccggg tatcgctgcg
     2161 gccgaaaccg tcggtgccga ggctcatcaa tccgcccgga acccacggtg cgatttggtc
     2221 cggcaccatt ttcatataat cggaaaccgc aataaacacg ccttgttcgt tctctaaaat
     2281 tgattcgaga taggattttt tcggcaccgc tgtgggatgg agcatgttcc aacgtttggt
     2341 gttcaaggcg tcgccacgca ataacttgta gctggtcgcg ctccagacat cggcgctcac
     2401 atcgaatttt tcggccaaga cttcttgggc ccgcaacgct tcgcgcagta ttgggccgct
     2461 gccgaacaga tgcgctttat attttttgcc cttggtcgcc gctttcacct tgtacaggcc
     2521 cttgaggatg ccttcttcag ccccctcagg catggccggc atctcatagc tctcattgta
     2581 aagcgtcaag taataaagac tttcttcgcc ctcggtgtaa agccgacgca aaccgtcctt
     2641 gacgatcacc gcgacctcgt aggcgaaggc ggcgtcgtag gtcagcaagg tcggaaccgt
     2701 gctggcaagt atatggctat ggccgtcttg gtgctgcagc ccttcgccgt tcaacgtggt
     2761 cctgccggcg gtggcgccca gcaggaagcc tttgctcttg ttgtcggcgg cggcccaaat
     2821 taaatcgcca atgcgctgaa aaccgaacat cgaatagtag aagtagaagg gtaccatatt
     2881 gacgccaaga ttggcgtagg cggcgccggc ggcgataaag gacgacatgg cgccggcctc
     2941 ggtgatgccc tcttcgagaa tttgaccatc tttcgtttcg ttgtagtaga gcagagtctc
     3001 cctatcgact ggttcgtaaa gctgcccttt ggaagaataa atgccaatct ggcgaaacaa
     3061 cgaatccatc ccgaaggtac gggcttcgtc ggggatgatc ggcacgatgc gcttgccgat
     3121 gccttcgtgg cgcaagagct tggttagcaa gcgcacggcc gccatggtgg tcgacatcga
     3181 atgaccgccc gaacccttat aaaattctgc gtactccgag tcggcgggga cgtttaaggg
     3241 ttgagcacgc accttacgtt cgggcaagaa cccgttcaac tgcttgcgcc gctccaacag
     3301 atactcggtc tccgggctgt cgataggcgg gcggtaaaaa ggcgtctcga cgacttcttc
     3361 atctggtagg gggatatcga agcgtgtacg aaactcgcgt aactcccgtt cgttcaattt
     3421 cttttgctgg tgggtgatat tgcggccttc gcccgcttcg cccagtccgt agcctttaac
     3481 ggtttttgcc agaattacgg tcggcgcgcc tttatgttgc accgcggcta aataggcgga
     3541 ataaactttc atcgggtcgt gtccgccgcg caggagcttg tgtatctgat cgtcggacat
     3601 gtggttgacc atctcgagca gcgcaggata cttgccgaaa aaatgctttc tggtatagct
     3661 gccgggctcc acggagtatt tctgataatc gccgtcgaca gcctcttcca tgcgcttgag
     3721 caagagcccg tcatggtcca tggcgagcag cgggtcccaa tccgagcccc aaattacttt
     3781 aatgacgttc caaccggcac cgcgaaacag cgcttcgagc tcttgaatta tcttgccatt
     3841 gccgcgcacc ggtccgtcca ggcgctgcag attgcagtta accacccaga ccaaattgtc
     3901 gagattttcc cgcgatgcca gagtcaacgc gccggaagat tccggctcat ccatttcgcc
     3961 gtcgccgacg aaacaaaata tccgtggctc ctcgccactg atcaatccac ggcttttcat
     4021 gtagcggacg aatcttgcct ggtaaatcga catgatcggt gccaggccca tggaaacggt
     4081 cgggaactgc cagaaatctg gcattaggta gggatgggga taggaggaag taccgccgac
     4141 actggagagc tcttggcgga agtgatgcaa cttcggcgcg tcgatgcgcc gttcgacata
     4201 ggcgcgagaa taaacccccg gcgacgcatg accctgaaaa taaaccatgt cgcctagatg
     4261 ggtgccgtca ctaccgcgaa agaaatgatt ttggccgact tcgtacagcg ttgccgacga
     4321 tgcgtaggtg gaaatatggc cgccgatatc ggcatgaagc cgattggctt tgaccaccat
     4381 ggccatcgcg ttccagcgca catagctctt gatgcgccgc tcgatctcac ggttgcccgg
     4441 atagggaggc tgcttttccg gcgggatcgt attgatatag ggggtgctga ctgccagagg
     4501 aatcttgaga ccggattggc gcaaacgatc ggatagctcc tcgaccagca gcgctgcttg
     4561 ttccggttgt tcgttcttta agataga
//
LOCUS       DASQKS010000410         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_645052, whole genome shotgun sequence.
ACCESSION   DASQKS010000410 DASQKS010000000
VERSION     DASQKS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645052"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VE170_10635"
     CDS             <1..416
                     /locus_tag="VE170_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15935.1"
                     /translation="PPASFQLEKMGFHQLTSLLDVPYQNVGIVIRRSRFDELRGRLLP
                     LLRSLRSGIDRYNSDKNFSLKVIAKYNKENDPDALDRSYEFYRRAGFRRELTISEPGV
                     QGMLDFLSETIPEAKKAKPAQFFDDRLVKQLDAGK"
     gene            517..1560
                     /locus_tag="VE170_10640"
     CDS             517..1560
                     /locus_tag="VE170_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ15936.1"
                     /translation="MSSITAIDADGHVIERESEIRKHLKAPWNLRTTALRPGDQPWDN
                     YMFGHFVTELTWSKLSAREQVGKWHEILDEHGIETAVCFPTGSGSVVRLQEIQFQIAV
                     ARACNDHFAADYNALSKRIKCVGVLPLRSPQAAAEELERAVKVLGITAFEILPVGLPF
                     ALGDSFYDPVYAAAERCGAVLGIHGTRSWSRELGAEGLNTFAEVHAYAFTAGILLQFT
                     SMICQGVPVRFPKLRFAFLEIGATWLPYYLDRLDEHWEKRAEVEMPLLKEKPSDLVRQ
                     SEVYFSIEPGESQLPQTIDYVGADHFVWASDIPHWDCEFPGNLEHLRNHGVLSADVRE
                     KILYKNAKRLFNL"
     gene            complement(1626..2090)
                     /locus_tag="VE170_10645"
     CDS             complement(1626..2090)
                     /locus_tag="VE170_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ15937.1"
                     /translation="MNFPLPEADAATAAEVRDWLDRFAVCVREVDYAAARPFWHPDIV
                     IFGTYQELVKSLSGWTEQQWDNVWPRTAEFRFDLASTMVMISPDGMLAVAIAPWTSTG
                     FHANGTPFDRPGRATIVLARQSDERWLGVHSHMSLARGVPQESHGKRAVKAR"
BASE COUNT          487 a          683 c          628 g          418 t
ORIGIN      
        1 gcccgccggc gagtttccaa ctggagaaaa tgggctttca ccaactgacc agcctattgg
       61 acgtgcccta tcaaaacgtc ggcatcgtca tccgccgcag ccgcttcgac gagttgaggg
      121 gccggctact gccgctgttg cgcagcttgc gcagcggtat cgatcgctac aacagcgaca
      181 agaacttcag cctcaaagtg atcgccaaat acaacaaaga aaacgacccc gatgcgctgg
      241 acaggtccta tgaattttac cgccgcgccg gctttcgccg cgaactgacg atctccgagc
      301 ccggcgtcca gggcatgctc gattttttat ccgagacgat tcccgaagcg aagaaggcca
      361 aaccggcgca gttcttcgac gatcgcctgg tcaagcagct ggacgcggga aaatgaattc
      421 gggcgactag agaaatgaga atgactgcct caccgcgaag gacgcataga gaaaggaaat
      481 ggctatctag cttccagtcg accaaggagt gaaaccatga gcagcataac cgccatcgac
      541 gccgacggcc atgtcatcga gagagaaagc gaaatccgca aacacttaaa agcgccttgg
      601 aatctgcgca cgacggcact gcggccgggc gaccaaccct gggataatta catgttcggc
      661 cactttgtca ccgagctgac ctggagcaag ctgtcggcgc gcgagcaggt cggcaaatgg
      721 cacgagatcc tcgacgagca tggcatcgag accgcggtct gttttcccac cggctccggc
      781 agcgtcgtcc gtcttcagga gatccagttt caaattgccg tggcgcgcgc ctgcaacgat
      841 catttcgccg ccgactacaa tgcgctgtcg aagcgcataa aatgcgtcgg cgtgttgccc
      901 ctgcgctcgc cgcaagcggc cgctgaggaa ttggaacgcg cggtaaaagt tctgggcatc
      961 accgccttcg agatcctgcc ggtcggcctg ccattcgcgt tgggcgattc tttttacgac
     1021 ccggtctacg ccgcggcgga acgctgcggc gccgtccttg gcattcacgg cacccgcagt
     1081 tggtcgcgcg aactcggcgc cgaagggcta aacacgttcg ccgaagtgca cgcctacgcc
     1141 tttaccgccg gcattctgct ccaattcacc agcatgatct gccaaggcgt gccggtgcgc
     1201 tttcccaagc tgcgcttcgc cttcctggag atcggcgcta cctggctgcc ctactatttg
     1261 gaccgtctcg acgagcactg ggagaaacgc gccgaagtcg agatgccgct gctgaaagaa
     1321 aaacccagcg acttggtgcg ccagtcggaa gtctatttca gcatcgagcc cggcgagtcg
     1381 caattaccgc agaccatcga ctacgtcggc gccgatcatt ttgtctgggc ctcggatatt
     1441 ccccactggg actgcgagtt tcccggcaac ctggaacact tgcgcaacca tggggtgctg
     1501 tccgcagacg tgagagagaa aatcctctac aaaaacgcca agcggctgtt caatctgtaa
     1561 acagcgcgat ggccatgccc gccattgaat cgggctattg aataatcgca agtgacgcaa
     1621 atcgtctaac gcgccttcac cgcccgtttc ccatgacttt cctggggcac gccgcgcgcc
     1681 agcgacatat gtgaatgcac gccgagccag cgctcgtccg attgccgcgc caggacgatt
     1741 gtcgcgcggc ccggccggtc gaagggtgtt ccgttggcgt ggaagcccgt gctagtccag
     1801 ggcgcgatcg cgaccgctag catgccgtcc ggggaaatca tgaccatggt gctggccaga
     1861 tcgaaacgga actccgccgt gcgcggccaa acgttatccc attgctgctc ggtccagcct
     1921 gacagacttt tcaccagctc ttggtaggtg ccgaagataa cgatgtcggg gtgccaaaac
     1981 gggcgcgccg ccgcgtagtc cacctcgcgg acacagacag cgaagcgatc gagccaatcg
     2041 cgcacctctg ccgcggtcgc ggcatcggcc tctggaagcg gaaagttcat tttactccaa
     2101 gcaaatgcta agtaccgtcc cgggtgccat taaactattt gccgcgattt tattgtcttg
     2161 ttcagcacga agaaagcgag agcgaagata gcgagattca aacccgcctg caactt
//
LOCUS       DASQKS010000411         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4834691, whole genome shotgun sequence.
ACCESSION   DASQKS010000411 DASQKS010000000
VERSION     DASQKS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4834691"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..821
                     /locus_tag="VE170_10650"
     CDS             102..821
                     /locus_tag="VE170_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYJ15938.1"
                     /translation="MSAPSKPKQAATVILLRPVEARGFEVFLTRRPDSMPFLGGMYCY
                     PGGTVSKDDCRPAMIARVVGRTPKQARQIIGAAFTPEEAIGLWIAAVREVFEEVGVLL
                     AAEYSGAGLSMTPERAARLAERQRELSNKSLSFAALLETERLYCDLSSLSHFSYWRTP
                     AQFAMRFDTRFFIAALPPGQTPLQTSYEVAHSVWLTPDRAMQLCNRHELPMIFPTFAA
                     LRTLADFDTLDSVLREFGLAK"
     gene            1013..2269
                     /locus_tag="VE170_10655"
     CDS             1013..2269
                     /locus_tag="VE170_10655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ15939.1"
                     /translation="MLRSFYYRIGGLYFGWRMVAVGAVLRVLGGGLYFYGFSVFFLPL
                     SQDLGLSRASTSLVFSLARAQGAFEGPFAGYFMDRHGPRPLMILAILCAGIGHMLLAG
                     VNSYVMLLLVYMGVVSLAFHAGFMDAPMLIANTWFIRQRTMAMAVISGSIGIGGFIFT
                     PFLSMAVHAYGWRPTAFACGITFIVVGLPLALLVRRSPESMGLQPDGDPLPPAVHDNY
                     NGPAHAAEEADFTLREALRTSAFWFITAATAVRVIILSAINVHYVALMVWTGMSEQRA
                     AFFLAAQAFMALPSHILFGWLGDKVDKPKLMAGCMLSAMVSMILLGVVQTEWAILLFT
                     ALFSTVESTFPVNWSTVGEYFGRKNFAKIRGSMSFVSTWGSVIGPVIAGLVYDQTKSY
                     ELLIWSSAGLLLLASALYAMVKKPLR"
     gene            complement(2319..>2834)
                     /locus_tag="VE170_10660"
     CDS             complement(2319..>2834)
                     /locus_tag="VE170_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15940.1"
                     /translation="VELPGAKSRDVSFGVFAARALQAGKVDGFWANAMGGATATSCGA
                     GKVLVDVRRGDDPAEVRYCTFAGMATTDDFIAREPEAMAAAVRAIVKAQKALRADPSL
                     ARVVGLGKFPADAAELIAAVVTRDAEFYDPAISEERIAKMNHFAASVGQLTAPVKYED
                     VVAVKFRESWK"
BASE COUNT          467 a          849 c          849 g          669 t
ORIGIN      
        1 gtggtcgcat tggcagcggc gcgggcatgc ttcgaaacat ttttttgtcc cggcccgcct
       61 tgtcaaaaca agtttatcgg cccggtgagt ttggcgattc gatgagcgca ccgagcaagc
      121 ccaaacaggc ggcgacggta attctcctgc ggccggttga ggcccgaggc ttcgaagtgt
      181 ttctgacccg ccggcccgac agcatgccat tcttgggcgg catgtactgc tatcccggag
      241 gcaccgtgag caaagacgat tgccggccgg caatgatcgc gcgtgtggtc ggccgtacgc
      301 ccaaacaggc gcgccagatc atcggcgccg ctttcacacc cgaagaagct atcggcctgt
      361 ggatcgccgc ggtgcgcgag gtcttcgagg aagtcggcgt gctcctcgcc gccgagtatt
      421 ccggcgcggg attgtccatg actccggaac gggccgcgcg cctagccgag cgccagcgcg
      481 aactgtcaaa taaatccctc agttttgccg cgttgctgga aaccgaacgg ctctactgcg
      541 atctctccag cctgagccac ttttcctatt ggcgaacgcc ggcacagttc gccatgcgct
      601 tcgacacgcg cttctttatt gccgctttgc cgccggggca aacgccactg cagacttcct
      661 acgaggtggc gcatagcgtc tggctgactc cagaccgcgc catgcagttg tgcaatcgcc
      721 atgagctgcc gatgatcttt ccgaccttcg cagcgctgcg cacgttagcc gacttcgaca
      781 cgttggacag cgtgctgcgc gagttcggcc tggctaagta gccggatggc caacccgtgg
      841 ggcgcccggc atatccccac tctcggtttg ccaaccttgc gtcatttgcc gccgagcccc
      901 ttcttttccc tgctttgttg aattcgccgg ctggctcggc tactttgaaa cctctcgcct
      961 gtcatcccgc acgggaactg ccggcattta gattcgtttc ataaggattc agttgctgcg
     1021 ttcgttttac tatcggattg gcggtctcta ttttggctgg cgcatggtcg ccgtcggcgc
     1081 ggtgctgcgt gtgttgggcg gtggattata cttttacggc ttctcggttt tctttctgcc
     1141 gttgagtcag gacttgggac tgagccgcgc ttcgacttca ctggtgtttt ccctggcccg
     1201 cgctcagggg gcttttgaag gaccgttcgc cggctacttc atggatcgcc acggcccgcg
     1261 gccattgatg atcctggcaa ttctttgcgc cggcatcggc catatgctgc ttgccggtgt
     1321 caacagctat gtcatgctgc tgctggtcta catgggcgtg gtgtcgctgg cgtttcacgc
     1381 cggtttcatg gatgcgccaa tgttgatcgc caatacctgg ttcatccgcc agcgcacgat
     1441 ggcgatggcg gtgatcagcg gctccatcgg tatcggcggt tttatcttta cgccgttctt
     1501 gtcgatggcg gtgcatgcat acggctggcg cccgacggcg tttgcctgcg gtatcacttt
     1561 tatcgtcgtc ggcttgccgt tggctttgct cgtgcggcgc tcgccggaga gcatggggct
     1621 gcaaccggac ggcgatccgc taccgcctgc ggtgcatgat aattataacg gaccggcgca
     1681 tgccgccgaa gaagccgact ttaccctgcg cgaagcgctg cgcacctcgg cattttggtt
     1741 tatcaccgcc gccacggcgg tgcgtgttat tatcttgagc gcgattaacg ttcactatgt
     1801 cgcgctgatg gtctggaccg gcatgagcga acagcgggcg gcgttttttc tcgccgccca
     1861 ggcgttcatg gcgctgccgt cgcatatctt gttcggctgg cttggtgaca aagtcgacaa
     1921 gcccaagtta atggccggtt gcatgctgtc ggcgatggta tcgatgatcc tcttgggcgt
     1981 ggtgcaaacc gagtgggcga ttttactttt caccgctttg ttcagcacgg tggagtcgac
     2041 ctttccagtc aactggtcaa cggtgggaga atatttcggc cggaaaaatt ttgctaagat
     2101 tcgcggctcg atgagcttcg tatcgacctg gggcagcgtc atcggcccgg tcatcgccgg
     2161 cctcgtctac gatcaaacta agagctatga attattgatc tggtcatcag cgggactgtt
     2221 gctgctcgcc agcgcgctat acgcaatggt gaaaaagccg ttacggtaaa aattgggtga
     2281 agcgtgtcgg aagtgatagc tttggtccct gacgattgtt atttccacga ttcccgaaac
     2341 ttgaccgcca ccacgtcttc atatttcacc ggcgccgtta gctgtccgac ggacgcggcg
     2401 aagtggttca ttttagcgat gcgttcttcg ctgattgccg ggtcgtagaa ctcggcgtca
     2461 cgtgtaacca cggcggcgat cagttcggcg gcgtctgcgg ggaatttgcc caggccgacc
     2521 acccgagcaa gcgacggatc ggcgcgcagc gctttctgcg ctttgacaat ggcgcgtacc
     2581 gccgccgcca ttgcttccgg ctcgcgcgcg atgaaatcat cagttgtcgc cataccggca
     2641 aacgtgcaat accgcacctc cgccggatcg tcgccacggc gcacgtcgac aagaactttc
     2701 cctgcgccgc agctggtggc ggtcgcgccg cccatggcgt tggcccagaa gccgtcgacc
     2761 ttaccggctt gcagcgcacg cgcggcgaac acgccgaagg acacgtcgcg cgatttcgcg
     2821 cccggtagtt cgac
//
LOCUS       DASQKS010000412         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5251759, whole genome shotgun sequence.
ACCESSION   DASQKS010000412 DASQKS010000000
VERSION     DASQKS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5251759"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VE170_10665"
     CDS             complement(<1..399)
                     /locus_tag="VE170_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYJ15941.1"
                     /translation="MKQRAPQLPKRQGIIFILSAPSGAGKTTLYNGLKKNYPDIQLSV
                     SCTTRAQRRGEVPGRDYRFLSEARFEALKSRGEFAEWAQVHDYRYGTPRKPIDRAIKA
                     GRDILLDIDVQGARKIKKAYPAAVAVFVLPP"
     gene            complement(396..1256)
                     /locus_tag="VE170_10670"
     CDS             complement(396..1256)
                     /locus_tag="VE170_10670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HYJ15942.1"
                     /translation="MKSMTGYGEASQNIHGARITVQIRSVNHRHLDLQVRTPREYMAF
                     EEEIRKLLREKIARGRIDLFVNRYVAKGQARKLEMDEALIGQFLSGVAQLKKKYKLGG
                     EVDVALVSNIPDVFHVRELEIDSKGERQAVLKALNGALKKLEQSREREGIHLRTDMEA
                     QIGHLREIAAALEARSLETGARQYKGGWPDGQNGERPERNMPEVATAIAKGDINEELV
                     RLKTHVVALAKVVREREPVGKKVDFMLQEVNRELNTITSKVPYLPVTQLVLQGKERVE
                     KIREQTQNIE"
     gene            complement(1253..2236)
                     /locus_tag="VE170_10675"
     CDS             complement(1253..2236)
                     /locus_tag="VE170_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYJ15943.1"
                     /translation="MNSLLDILARFKGAHLLVVGDLMLDRFVLGDVERLSPEAPVPVL
                     RVVSEDRRLGGAANVVHNIRALGGRVTACGIIGNDDAGKRILAILRRVGASTAGIFTD
                     RKFQTIQKSRIIASPHHQQIVRLDRESREPIHGATLKRLREFILDRAADCDGVLISDY
                     GKGAVHAELLSALAGQAGKRKMLYVVDPKKENFRHYRFPTLITPNKSEAADAAAIDID
                     DDRTLFAAGTKLLRLWRAQGVLITRGADGMSLFRPRSKPRHFPTAPREVFEVTGAGDT
                     VVAVCALVLACGGDFAAAAMVANLAAGFVGDEIGTVAVPFKKLKQKIEELT"
     gene            complement(2259..3374)
                     /locus_tag="VE170_10680"
     CDS             complement(2259..3374)
                     /locus_tag="VE170_10680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ15944.1"
                     /translation="MTTSGARDGRKLLKILHVDPERNWGGGEAQVFGLLSYLAAQGHR
                     NDLLTHPEGRLFDEARGFTVDLLPLVVRNDLDLRAIPKLRRQIAAANYDVVHLHTKRA
                     HALSLWLPRGDPSPRYVVTRRMDYAEKKNWRTRCLYNRRVDGVVAISQTIVELLVVAG
                     VERRKIRLIHSGIDPSRFAAAQLPRDSDHEVINVGCLAVLEERKGIDFLLQAAARLQS
                     QGVRLNWLIGGDGSQRGALASKARALGLDERVNFLGFVSKPEDFLARIDIFVMPSLFE
                     GLGVAALEAMAAGKPVVATRVGGLAESVLDGRTGILVAPRDSSGLADAIARLAGDPPL
                     AQRMGLQGRQRALEKFTLTQMAAQNEAYYYELLEMGN"
     gene            complement(3352..4140)
                     /locus_tag="VE170_10685"
     CDS             complement(3352..4140)
                     /locus_tag="VE170_10685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ15945.1"
                     /translation="MARPSFTISAIVVCFNEEERIEDCLKSLVWCDEIVVVDAFSSDR
                     TPEICQRYTQRFVQRAWAGYRDQKAYAHSLAAMDWVLLVDSDERVTDALRNEIVESLT
                     QDAGLYAGYAIPRLVHYLNRWWWRGGWYPDYDVRLFRRDRATWGGVDPHEKILVDGKV
                     RRLRQPLHHFSYRSIDDHIQRINRFTSISAAELRKSGGRWRLSDALLRPAVRFFRSYI
                     LKRGFMDGFAGFHVALTAAVYVFLKYAKLWELEIKEKRDDERRP"
     gene            4214..4957
                     /locus_tag="VE170_10690"
     CDS             4214..4957
                     /locus_tag="VE170_10690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ15946.1"
                     /translation="MKNLESVYQLPGMLTTHEVDCLYRLGQFSEQKGVIVEIGSWKGK
                     STVALALGAAKVNAEKIYAIDPHRIMPEEGYLEDTEADFLANIKQAGVENQIVPMVMT
                     SEAAAQHWDKPIRLLWIDGDHRYEPTKLDFSLWEPHLVEGGILAMHDTIRKNGPKRVL
                     WEKVFRSDRFQEITIVDNITAVRKVRKASFEAKCLKALTLSTRAAYIAARKIGVPKSK
                     EIGRTLLSRVTAQTWTRLLVFVFSASIAR"
     gene            complement(4903..5658)
                     /locus_tag="VE170_10695"
     CDS             complement(4903..5658)
                     /locus_tag="VE170_10695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ15947.1"
                     /translation="MEQVVEGFRGSGRAEFRIVEEPLPGKCRAQNAAIKLARGEIIAF
                     FDDDVVVTPEWLKVAADFFKHESFDVMQGPILVPPEIEHDENFIQAQRRFRTINFVKY
                     PLGLKEIKTLTGANMAVRREVFSKIGVFNEELGPGRSGISEDVEFAKRVLKAGGRIGY
                     EHRAAVYHIVDWSRLTEEFFRDRHEQQGRSRLIYKKQSLTSIVPNLLRSMWTLAWYSM
                     MGDIRQKYRAKGRYFHYRAMLAEKTKTSSRVQV"
BASE COUNT         1230 a         1717 c         1518 g         1199 t
ORIGIN      
        1 cgggggcaaa acgaataccg ccaccgccgc tgggtaagct tttttgattt tgcgcgctcc
       61 ctggacatcg atatcgagca gaatgtcgcg gcccgccttg atcgcgcgat cgatgggctt
      121 tcgcggcgtg ccatagcgat agtcatgcac ctgagcccat tcggcgaact caccgcggct
      181 tttcaaagcc tcgaatcgcg cctcgctcag aaaacgatag tctcgccccg gcacctcgcc
      241 gcggcgctgc gcgcgcgtag tgcaggaaac cgaaagttga atatccggat aatttttttt
      301 tagcccgtta taaagggtcg tcttgccggc tcccgacggt gccgaaagaa taaatataat
      361 cccctgcctt ttaggcagtt gaggtgcgcg ctgtttcatt cgatgttttg ggtttgctca
      421 cgaattttct cgacccgctc tttgccttgc agcaccagtt gggtaaccgg cagatagggc
      481 accttggatg tgatggtgtt gagttcgcga ttgacctctt gaagcatgaa gtcgaccttt
      541 ttgcccaccg gttcacgttc gcgcacgacc ttggccagcg ccacgacatg ggtctttaaa
      601 cgaaccaatt cctcgttgat gtcgcctttc gctatcgcgg tggcaacctc cggcatattc
      661 cgctccggcc gctcgccgtt ctgtccatcc ggccagccgc ccttgtactg gcgcgctccg
      721 gtctcaagcg agcgcgcttc cagagccgcg gcgatttctc gcaaatgacc gatctgggcc
      781 tccatgtcgg tgcgcaaatg aataccctcg cgctcgcgcg actgctcgag tttcttgagc
      841 gcgccgttga gcgccttgag caccgcctgg cgctcgcctt tgctgtcgat ttcaagctcg
      901 cggacatgga aaacatcggg aatgttggaa accagtgcga catcgacttc gccgccgagt
      961 ttgtacttct tcttgagctg agcgaccccc gaaaggaatt gaccgatcag agcctcatcc
     1021 atctcgagct tgcgggcttg ccctttggcc acatagcggt tgacgaacaa atcgatgcgg
     1081 ccgcgggcga ttttttcgcg caggagcttg cgaatttctt cctcgaacgc catatactcg
     1141 cgcggcgtgc gcacttgcag gtccagatgg cggtgattga cgctgcggat ctgcacggtg
     1201 attcgagcgc cgtgaatatt ctgcgacgcc tcgccatagc cggtcatgct tttcatgtga
     1261 gctcctcgat cttctgcttg agctttttga aaggcaccgc cacggtgccg atttcgtcgc
     1321 cgacaaaccc cgccgccagg ttggcaacca tcgcggcggc cgcaaagtcg ccgccgcagg
     1381 ccagcaccaa cgcgcacacc gccaccaccg tatcgccggc gccggtcacc tcgaaaacct
     1441 cgcgcggcgc ggtgggaaaa tggcgcggct tggaacgagg ccgaaacaag ctcatgccgt
     1501 ccgcgccgcg agtaatcaat accccctggg cacgccatag gcgcagtaat tttgtcccgg
     1561 cggcgaacag cgtccggtca tcgtcgatgt cgatggcggc ggcatccgcc gcttcgcttt
     1621 tattgggagt aatcaaggtc ggaaatcgat aatggcgaaa attttctttc ttgggatcga
     1681 cgacatagag catttttcgc ttgcccgctt gaccggcaag cgccgacaac agctcggcat
     1741 gcacagcccc cttgccgtaa tccgagatca agacgccgtc gcaatccgca gcccgatcga
     1801 gaataaattc gcgcagccgt ttcaacgtcg caccgtgaat cggctcgcgg ctctcccggt
     1861 cgagccggac aatctgttgg tggtgcgggc tggcgataat tcggctcttc tgaatggttt
     1921 gaaacttgcg atcggtaaag attccagccg tcgacgcgcc aacgcgccgc aggatggcga
     1981 ggatacgctt gccggcatcg tcattgccga taatgccgca ggcggtgacc cggccgccca
     2041 atgcgcgaat gttgtggaca acgttcgccg cgccgccgag acggcgatcc tcggacacca
     2101 cccgcagtac cggcaccggc gcttccggcg acaaccgctc gacatcgccg agaacaaaac
     2161 ggtcgagcat cagatcgccg accaccaaca aatgcgcgcc tttgaatctt gcgagtatat
     2221 cgaggaggct gttcattcga agcaaagtga gacggccgct aatttcccat ctctagtaat
     2281 tcgtagtaat acgcttcgtt ctgcgccgcc atttgcgtca gggtgaactt ttccagcgcc
     2341 cgctgccggc cctgcaatcc catccgttgc gccaatggcg gatcgccggc tagtcgggcg
     2401 atggcatcgg cgagcccgct gctgtcgcgc ggcgcgacca atatccccgt tcgtccgtcc
     2461 agcaccgatt cggccaatcc accgactctg gtggcaacca ctggtttgcc cgccgccatc
     2521 gcttccagag ccgccacccc caagccctcg aacaacgacg gcatgacgaa tatatcgata
     2581 cgcgcgagaa agtcctcagg cttggaaacg aagcctagga agtttacccg ttcgtcgaga
     2641 ccaagcgccc gcgctttgct tgccagggct ccgcgctgag acccgtcccc gccgatcagc
     2701 cagttgagcc gtactccttg agattgcaat cgcgccgccg cttggagcag gaagtcgatc
     2761 cccttacgct cttccaggac agcgagacac cccacattta taacctcgtg gtcagagtcc
     2821 cgcggcaatt gcgcggcggc aaaccggctc gggtcgatac cgctatgaat cagccgaatc
     2881 ttgcgccgct ccaccccggc aacgaccagt agctcgacga tggtttgcga gatcgcgacc
     2941 acgccatcga cccggcgatt gtataggcag cgggtgcgcc aatttttttt ctcagcataa
     3001 tccatccggc gcgtgacgac gtaccgtgga ctcggatcgc cgcgcggcaa ccacaaagac
     3061 agggcatggg ctctcttggt gtggagatga accacatcgt agttggccgc ggcgatctgc
     3121 cggcgcagct tcgggatcgc ccgcagatcc aaatcgttgc gcaccaccaa gggcaacagg
     3181 tccaccgtaa atccgcgggc ttcatcgaac aaacggcctt ccggatgggt caatagatcg
     3241 ttacgatgcc cctgcgccgc aaggtaggaa agcaagccaa agacttgtgc ctccccgcca
     3301 ccccagttcc gttccggatc gacgtgtaga atcttcagca gcttgcggcc atcacgggcg
     3361 ccgctcgtcg tcacgtttct ccttgatttc aagttcccac aatttagcat acttcaagaa
     3421 tacgtaaacc gccgcagtca gcgccacatg gaagccggcg aacccatcca tgaacccgcg
     3481 ctttagaatg taggagcgaa aaaaccgcac cgccggccgc agcaaagcat cgctcagccg
     3541 ccaccggccg ccggattttc ggagctctgc cgccgagatc gaggtaaaac gattgattcg
     3601 ttgaatatgg tcgtcgatgc ttcgataaga gaagtgatgc aacggctggc gcaaccggcg
     3661 caccttgcca tcgacaagta ttttctcatg gggatcgacc ccaccccagg tcgcccggtc
     3721 gcgccggaac aagcgcacgt cataatccgg gtaccagccg ccgcgccacc accagcgatt
     3781 aagataatgc accaaacggg gaattgcgta acctgcgtaa aggcccgcgt cctgggtcag
     3841 actctcgaca atctcgtttc tcaatgcgtc ggtcacccgt tcatcggagt caaccagtaa
     3901 cacccaatcc atggccgcca aggaatgagc gtaggccttt tgatctctgt agccggccca
     3961 tgcgcgctgg acaaatcgtt gggtgtagcg ctgacaaatc tccggcgtac gatcgctgct
     4021 gaaggcatcg acaacgacga tctcatcgca ccaaacgaga ctctttagac aatcctcgat
     4081 gcgttcttct tcgttaaaac aaaccacaat tgccgaaatg gtaaatgaag gtcgggccat
     4141 gcgactaaag cttgcgtcaa attctcttca ttatgacagg cgggtttgct ataagtggcg
     4201 caggagtatc gccgtgaaaa atctcgagag tgtttaccaa ctgcccggca tgctgacgac
     4261 gcacgaggtc gattgcctgt atcgactagg tcaattcagc gagcaaaaag gtgtcatcgt
     4321 cgaaatcggc agctggaaag gcaagtccac cgttgccctc gccctcggcg ccgcaaaagt
     4381 caacgcagaa aagatttacg ccatcgatcc ccatcggatt atgccggaag aaggctacct
     4441 ggaagacacc gaagcggatt ttttggcgaa tataaaacag gccggagtcg agaatcagat
     4501 tgtcccaatg gttatgacct cggaagcggc ggcgcaacat tgggataagc cgattcgcct
     4561 actttggatc gacggcgacc atcgctacga accgaccaag ctcgactttt cgttgtggga
     4621 gccgcattta gtcgaaggcg gaatcttggc catgcacgat acgattagaa aaaatggacc
     4681 taagcgcgtg ctgtgggaaa aggtttttcg gtcggatcgt ttccaagaaa tcaccatcgt
     4741 agacaacatc acggcggtga gaaaggtgag aaaagcgtcc ttcgaagcca aatgtctcaa
     4801 ggcgctgact ctttccacgc gcgccgctta tatcgccgcg cggaaaatcg gcgtccccaa
     4861 atccaaagag atcggcagga cgcttctgag cagagtcacg gctcaaacct ggacccgact
     4921 gctggttttt gtcttctcag cgagcatcgc gcggtaatga aaatagcgtc ccttggcgcg
     4981 atatttttgt cggatgtcac ccatcattga ataccacgcc agtgtccaca tggaacgcag
     5041 caggttcggg acgatggacg ttaaagactg ctttttataa ataagccgac tgcgcccttg
     5101 ctgttcgtgg cgatcgcgaa aaaactcctc agtcaacctg ctccagtcaa cgatgtgata
     5161 gaccgcggca cggtgctcgt aaccgatccg ccctccggct ttgaggacgc gtttggcgaa
     5221 ttcgacatcc tcggatatgc cggatctacc aggacccagt tcctcattga atacaccgat
     5281 ttttgaaaac acttcccggc gcaccgccat attggcgccg gtgagcgttt taatctcctt
     5341 gaggcccaat ggatatttaa caaaatttat ggttctgaac ctcctctgag cctgaatgaa
     5401 attttcatca tgttcgattt ccggcggcac aaggatcggc ccttgcatga cgtcgaaact
     5461 ttcgtgcttg aaaaaatctg ccgccacctt taaccactcg ggagtgacga cgacatcatc
     5521 gtcgaaaaaa gcgattatct cgcctcgcgc caatttgatc gccgcatttt gcgcgcgaca
     5581 cttgcctggg agaggttctt caactatgcg aaactcggct cgtccggagc cgcggaaacc
     5641 ctcgacgacc tgctcgattt tttt
//
LOCUS       DASQKS010000413         5277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4414929, whole genome shotgun sequence.
ACCESSION   DASQKS010000413 DASQKS010000000
VERSION     DASQKS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4414929"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VE170_10700"
     CDS             <1..764
                     /locus_tag="VE170_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15948.1"
                     /translation="ITGGPVAMQALLAKSLDIITTSATVFLHGFVEGADVKIIGGVNN
                     RFPYTFFSRPNITTAAQLKGKVIGITRYGSTDELATRMALEQFGLNARTDVKFIQGGG
                     SAGRINALQAGSIDGTSLISGVSHVAKKAGMNPMLDFAEKEIDYQMTGVVARGDYLKS
                     NPDALRRFLRAYVESIRYYKSNRADAIRETMRAIRTDDRQLAEADYNFRARAFPDDGK
                     PTLKGIQLAIDELAKENPKAKTVTPQQVIDLSFLP"
     gene            796..1695
                     /locus_tag="VE170_10705"
     CDS             796..1695
                     /locus_tag="VE170_10705"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HYJ15949.1"
                     /translation="MSNPTIGFIGLGIMGKPMARHLLQASYPLVVHNRSRAAVEELSK
                     EGAQSAASSQEVAQHSEVIITMLPDSPDVELVYAGERGIFAGMKSGALLIDMSSISPV
                     VARKLAAEAEKRSCDMLDAPVSGGEAGAINAALSIMIGGKNSAVQRAMPIFEKLGKNI
                     VHVGDAGAGQVTKAANQMVVGTTIAIVSEALVLAAKAGVDPAKVRQALLGGFAQSKIL
                     EAHGQKMLDRNFKPGFRIRLHEKDMKIALATGSEYGVPLMVTGVVGQMMTAMKGMGNG
                     DLDHAGLVKFVEELAKRELPNAK"
     gene            1963..2412
                     /locus_tag="VE170_10710"
     CDS             1963..2412
                     /locus_tag="VE170_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15950.1"
                     /translation="MRREFLRGTIPCFLLAWAVLAQAAELRSFYDGKTINLIVSTSPG
                     GATDVAGRLVSRHLGKYIPGHPNILAQNMAGAGGIVSANYLYNVAKPDGLTILAVNRA
                     NYLEQMVGRPEVKLDFQQAQLDRKFQPRAHDDHLPQGFGLHHHRRFA"
     gene            2598..3011
                     /locus_tag="VE170_10715"
     CDS             2598..3011
                     /locus_tag="VE170_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15951.1"
                     /translation="MRPPWPTWAQQGFMVFVVQQGPSNSRLLPQTTPTVYDLAPSTAK
                     AALGLMDVMVAYTDFDRPFAAPPAIPRERLQVLRDAFEKMLEDANFMLEAKKLVDWDG
                     TSHLSGVELQIKIAKTITQPPSVIQRIKDVLKESS"
     gene            3424..3846
                     /locus_tag="VE170_10720"
     CDS             3424..3846
                     /locus_tag="VE170_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15952.1"
                     /translation="MKKNLALLLMFLCATFMAIVPSHGNEFKEREAKIAQYKKWLDTL
                     GPRGSQYWLRLDSTRRPHRLYLGEAFRRADAQSQEQFVDKYSNYLAGHPEKFMLIDLF
                     DADTSKLLGEYGFGGFKMYIEAAPPDSDNLKIDATDTE"
     gene            complement(3969..4961)
                     /locus_tag="VE170_10725"
     CDS             complement(3969..4961)
                     /locus_tag="VE170_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15953.1"
                     /translation="MTRTSLNISTFSAAAFGAILLTSKFAIAQPLQIVNMAIPARSFQ
                     MVIYPVAQQKGYMKEEGIDQRVIFVAPTTSIQAMLGGDIHFTGAGSSALVSVARGNTP
                     LKVVLATNDRVLQWFVTKPEITSPKDLKGKRIATTGVAAIATFMARQVLTKHGLDGNK
                     DVTYFDVGQGNQLAALLGGGFDAAILSVEQRYVALDKGMRELFFMGNEVKNSWGTLAT
                     TDKLIKENPKMVGGFMRATIKALRYIRQDKEGTISAMVKFANVSRQQANRVYDDIIPT
                     FTRSGMVDDETQRNDLAIIRQVINGNETLPNARGYDFSFASEADAQLNKSGWKP"
BASE COUNT         1186 a         1529 c         1458 g         1104 t
ORIGIN      
        1 tgattaccgg cgggccggtg gccatgcagg ccttgctggc gaaaagcctc gacatcatta
       61 caaccagcgc gacggtgttt ttgcacggct ttgtcgaagg cgccgatgtg aagatcatcg
      121 gcggcgtcaa caaccgcttt ccctacactt tcttttcccg tcctaacatc acgacggcgg
      181 cacagctcaa aggcaaagtt atcggaatta cccgctatgg tagcaccgat gagttggcga
      241 cccggatggc gctggaacag ttcggcttga atgcaagaac cgacgtgaaa ttcatccaag
      301 gaggcggttc ggcgggacgc atcaacgcgc tccaggccgg cagcatcgat gggacatcgc
      361 taatctccgg cgtcagccat gtggcgaaga aggccggcat gaatcccatg ctcgacttcg
      421 ccgagaaaga gatcgactat caaatgaccg gcgtggtcgc ccgcggcgat tacttgaaga
      481 gcaatcccga tgccctccgg cgtttcctgc gcgcctacgt cgaatcgatc cgttactata
      541 aaagcaaccg cgccgacgcg atcagagaaa ccatgcgagc gatccgcacc gacgaccgcc
      601 agctggccga ggccgattat aatttccgcg cccgcgcctt ccccgacgac ggcaagccga
      661 cattgaaggg aatccagctg gcaatcgacg agctggcgaa agaaaatccc aaagccaaga
      721 ccgtcacgcc gcagcaagtg atcgatctga gtttcttgcc gtaaaacttt accagcaagc
      781 gatggagaat atcgtatgag caatccgacc atcggtttta tcggtctcgg catcatgggc
      841 aaacccatgg cgcgacatct gttgcaagcg agttatccgc tggtggtcca caaccgcagc
      901 cgcgcggcgg tggaggagtt gagcaaggag ggcgcacaat ccgcggcaag ctctcaagaa
      961 gtcgcccagc acagcgaggt catcatcacc atgctgcccg actcgccgga tgtcgaactc
     1021 gtctacgccg gcgagcgcgg catcttcgcc ggtatgaaat ccggcgccct gttgatcgat
     1081 atgtcgagca tttccccggt cgtcgcccgc aagctcgcag ccgaagcgga aaaacgcagc
     1141 tgcgacatgc tcgacgcccc ggtgagcggc ggcgaagccg gcgcgatcaa cgcagcatta
     1201 tcgatcatga tcggcggcaa gaatagtgcc gtccagcgtg ccatgccaat cttcgaaaag
     1261 cttggcaaga acatcgtaca tgtcggcgac gccggcgccg ggcaggtgac caaagcggcg
     1321 aaccaaatgg tcgtcggcac gacaatcgcc atcgtcagcg aagcgcttgt gctcgcggct
     1381 aaggccggcg tcgatccggc gaaagtcaga caagcgctgc tcggcggctt cgcccaatcg
     1441 aaaatcctcg aggcccatgg acagaaaatg ttggatcgca acttcaaacc cggtttccgc
     1501 attcgcttac acgagaaaga catgaagatc gcgctggcga ccggttcgga gtacggcgtc
     1561 ccgctcatgg tcaccggcgt cgtcggccaa atgatgaccg ccatgaaagg aatgggcaat
     1621 ggcgacttgg atcatgccgg gttggtgaag ttcgtcgagg aattggccaa gcgagaatta
     1681 ccgaacgcga aatgatgtgg tagacacggc ctccgactcg agcgcttggt cattcctgat
     1741 tgcaccaaga tcgagccccg cactggtagc attcaccgcg atcgtgagga atgcaaacct
     1801 ggcagcggtt tcgcttcacg cacggccggc cggcacagca gcttcactca cgcggacgct
     1861 tggaatgaga aaaccacgcg gggcaatttt gatggtaaac cttgaccgcc ggttgactac
     1921 cgtgacacag cgcgaagcgg tgtgattggg aggttgacga cgatgcgccg agagtttttg
     1981 cgaggaacca tcccatgctt cctcctcgct tgggcggtgc tcgcgcaagc tgcggaactt
     2041 cgttcctttt acgacggcaa gaccatcaac ctgatcgtct cgacctcacc cggcggcgcc
     2101 accgacgtcg ccggacggtt ggtgtcgcgc catcttggca aatacattcc gggccatccc
     2161 aatattctgg cgcagaacat ggccggcgcc ggcggtatcg tctccgccaa ctatctgtac
     2221 aacgttgcca aaccggatgg cttgacgatt ttggcggtca atcgcgccaa ttacctcgaa
     2281 caaatggtcg gccggccgga ggttaagctg gatttccagc aagctcaatt ggatcggaag
     2341 tttcaaccgc gcgcccatga tgatcacttg ccgcaaggat tcgggcttca ccaccatcga
     2401 cgctttgcgt agcgcgaaga atcccgcacg cttcggtgaa ggcggcacgg gcagtatcat
     2461 tatgtgttca gtagtttgct cggcgagatc ttcgatttca aagtcaaaca tgtcaccggg
     2521 tacggctcgg cgcgggaatc gatctcggca tagagcgcgg tgaggccgac tgccgcgcca
     2581 ccaccgacat cgcgctggtg cgccctccgt ggccaacctg ggcgcaacag ggcttcatgg
     2641 tgttcgtcgt ccagcaaggc ccgtccaata gccggctgct gccccaaaca acgccgacgg
     2701 tctatgatct ggcgccgtcc actgcgaaag cggctttggg tctgatggac gtgatggtag
     2761 cctatactga cttcgaccgt cccttcgccg caccaccggc cattccgagg gagagactac
     2821 aagttctgcg cgacgccttt gaaaaaatgc tggaggacgc gaatttcatg ctggaagcca
     2881 agaagctcgt cgattgggac ggcaccagcc atttgagcgg cgtcgagctg caaatcaaga
     2941 tcgcgaaaac catcacccaa ccacccagcg tcatccaacg gatcaaagat gtcctgaaag
     3001 aatcctcata gatacctgac cgttcaacgg ctaccttctt ggcgagaggc aaacgtttcg
     3061 ccgctatccg tcctccgaca ttatgcgcga atgattaccc ttcttgcttg gccttgagcg
     3121 aacgatacat cctagcctgg ccgcagcaac ctaaggtttt cacttgcgaa atacgttgcg
     3181 aatcccaggg ataacgtgga cgcttttcgt cccgcgcggg caatattgtt caatgacgcc
     3241 gaccaataaa acctttcaag tccagctagg gatcgagcag tcccggactt cgtctcgcaa
     3301 ctaatgacaa ttgcacctga attggttcac tgttaccagt tattgccagc agcgtggaaa
     3361 acattcctgt agtggccgca gccttgcgtc aagaattgcc agtatcttcc atggaggcaa
     3421 cacatgaaaa aaaatctagc cctgcttctg atgtttctct gcgccacgtt catggccata
     3481 gtgccgtcac atggcaacga attcaaagaa cgggaggcca agatcgccca gtacaagaaa
     3541 tggctcgata cgctcggccc tcgaggttcc cagtattggc tacggctcga ttccacacgc
     3601 cgtccccatc gcttgtactt gggcgaggct tttcgccgcg ccgatgcgca aagccaagag
     3661 caattcgtcg acaagtactc caattacctc gccggccatc cggaaaaatt catgttgatt
     3721 gatctcttcg atgccgatac cagcaaactg ttgggcgaat acggctttgg cggcttcaag
     3781 atgtatatcg aagcggcgcc gcctgactct gataatttaa aaatagacgc caccgacacc
     3841 gagtgaaggc gggcgtgcac ccgcgagtcg aacaagtgat tgcagatctt aagagtacgt
     3901 gttggccagc aatggttgaa aacgccgacg cgattgttca tccgcagtat ccgccttcaa
     3961 cttgattact acggtttcca gccgctcttg ttgagttggg catcggcctc cgacgcgaag
     4021 ctgaagtcat aacccctggc gttaggtagg gtttcgttgc cgttgatgac ctgacggatg
     4081 atggcgagat cgttgcgctg ggtttcgtcg tcgaccatgc cgctgcgagt gaaggtcggt
     4141 atgatgtcgt cgtagacgcg gttggcctgc tggcgcgaga cgttggcgaa tttcaccatc
     4201 gccgaaatcg tgccttcttt gtcctgacgg atatagcgca acgctttgat ggtcgcgcgc
     4261 atgaagccgc cgaccatttt gggattctcc ttgataagtt tgtcggtggt cgccaacgta
     4321 ccccaggagt ttttcacttc attgcccata aaaaacagct cgcgcatgcc tttgtccaag
     4381 gcaacgtagc gctgctcgac gctgagaatt gccgcgtcga aaccgccgcc gagtaatgcc
     4441 gcgagctgat tgccctgtcc gacatcgaag taagtcacat ctttattgcc gtcgaggcca
     4501 tgcttggtga gcacctggcg cgccataaac gttgcgatgg cggcgacccc ggtggtggcg
     4561 attcttttac ctttcagatc tttcgggctg gtaatttccg gcttggtgac gaaccactgg
     4621 agcactctgt cgttggtcgc aagcacgact ttcaacggcg tattgccgcg tgcgacgctg
     4681 accaaggcgc tgctgccggc gccggtgaaa tgaatatcgc cgccgagcat cgcctggatg
     4741 ctcgtggtcg gagcgacgaa gatcaccctc tgatcaatcc cttcctcttt catgtacccc
     4801 ttctgctggg cgaccgggta gatgaccatc tggaagctgc gcgccggaat cgccatgttg
     4861 acgatctgca gtggctgggc gatggcaaac ttggaagtta acagaatagc gccaaacgcg
     4921 gcggctgaaa aagttgaaat atttagagaa gtacgtgtca tgaccaactc ctctctctgc
     4981 aatgttatcc cgacggtggg tttgcaaccc aactggacaa agcaatgtct atttgcgttc
     5041 tttgccgatt atcgtcaaag gcaggctgcc gccccttctt tcatccttct ccactcacgg
     5101 tgtcccgctc tcccacaact agctcttgcg caattgagta atcgctactt ggcaactgcg
     5161 cgatggggtt cgcaaggttg acggtctgcg atacttgcct gacccaaatg cggcgccaca
     5221 agaaataccg ataaaatcaa gaatgccaat tatgccagca tagctctaac ctgaacg
//
LOCUS       DASQKS010000414         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1488084, whole genome shotgun sequence.
ACCESSION   DASQKS010000414 DASQKS010000000
VERSION     DASQKS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488084"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VE170_10730"
     CDS             complement(<1..1497)
                     /locus_tag="VE170_10730"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ15954.1"
                     /translation="MPFVALVVRDLFDESKNMPINDLREFIDAARALGEVKDIHGAHW
                     ELEIGALTELFAFKEPSPLVLFDQVPGYAPNFRVASNLINTPSRSSLTVGMPPNARPI
                     ELIAKWKELLKGVKPVPPRLVSSGPILDNVKSGADVDMTIFPTPHWHELDGGRYIGTA
                     DCVITGEPGEGGWVNVGIYRVQIHDKNTLGLYVSPGHHARIMREKYWEQGKSCPVVVT
                     FGQDPLLFLVAGQSMPYGLSEFDYYGGLRGAPLDVIRGELTGLPIPAHAEIAIEGEVP
                     PPQEQMQMEGPFGEWPGYYAHGAAKEPVIRVKRVYYRNDPILCGAPPLKPPYVTFGVP
                     IGAAAIWNYLEKADVPDIKGVWCAIGSSAAAGGAPFIIVSVKQRYHGHAQQAGLAALA
                     SRGGNYHGRFVIVVDDDIDPSDMKDVIWAVSTRCDPKTAISIIDGCWSTPLDPAMHPD
                     QRDAGNFVNSRAVLNACRPYAWRDRFPPVNALSPELRQQITEKWKKELA"
     gene            complement(1548..2342)
                     /locus_tag="VE170_10735"
     CDS             complement(1548..2342)
                     /locus_tag="VE170_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15955.1"
                     /translation="MNQNPLVLEIGKKHSPAEAKAVTDAIKQRMYALWDEKVFGGAFM
                     RDLEAGRLPFETLKLFWRNWYSYPVEVNNFHLIIYQRHQGIFARHRDLLAPYVGKISD
                     ELVNPKIPGHIEVLIKQGEQFGVSLDEMINCQVYAECRALTEFARGLVYEGSMIEWWA
                     RSLNEEMFGHWAKRWREALLGKYHVKEADLHYFAVHEEADLHEHLDGLMAHGEVARQI
                     FERLLQDGNTWYRPGWGPEYCCLTNAECVAMFHDGIYKHAKELGHF"
     gene            complement(2474..3391)
                     /locus_tag="VE170_10740"
     CDS             complement(2474..3391)
                     /locus_tag="VE170_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ15956.1"
                     /translation="MASNRKIEGILMPMPVAFKHDGAIDTKATDAIIDFYMDAGVHGF
                     FPLGTHGQGMVMEIDERKKVAEQIVNRVRGRVPVVMHIGTANTLSSIELAKHAVGLGV
                     DAVGVVPPYYYPHDDWEVYAHYKAIAQAVPGTPMFIYDNTETTWVEITPAKANKIIEA
                     IAPNPLAGIKVSFIAYDKLLGYVYQLPKTTGVFPGGIFSLYSGYSVGVRGAIHPPSTP
                     FPEACVKMYEALKADDLKAARTEHDLLTRLMQVVGKYLRSHGRGVFCELMKLRGLPVE
                     RFPRWETPPFTDKDRAELKEGLENAGFKG"
     gene            3552..>3818
                     /locus_tag="VE170_10745"
     CDS             3552..>3818
                     /locus_tag="VE170_10745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020442935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ15957.1"
                     /translation="MADKNPVVLMSTSMGDIRIELDAANAPITTKNFVDYVNAGHYDG
                     LIFHRVIPGFMIQGGGMDAAMHEKANKAPIKNEAANGLKNELGTL"
BASE COUNT          810 a         1067 c         1108 g          833 t
ORIGIN      
        1 tgccagctct tttttccact tctcggtgat ctgttgtctg agctcggggc tgagcgcgtt
       61 gaccggcggg aagcggtcgc gccaggcgta gggacggcaa gcgttgagca ccgcccggct
      121 gttgacgaag ttaccggcat cgcgctggtc agggtgcatc gccggatcga gcggcgtact
      181 ccaacagccg tcgatgatgc tgatggcggt cttgggatcg cagcgggtcg acaccgccca
      241 gatcacatct ttcatatcgg acggatcgat gtcgtcatcg acgacgataa cgaagcggcc
      301 atggtagttg ccgccgcgtg aggccagcgc ggcaagtccc gcctgttgcg cgtggccgtg
      361 atagcgctgc ttcaccgaga cgatgataaa cggcgcaccg ccggcggccg ccgagctgcc
      421 gatggcacac cagacgcctt tgatgtcggg cacatcggct ttttccaaat aattccagat
      481 cgcggcggcg ccgatgggca cgccgaatgt gacgtagggc ggcttcagcg gcggcgcacc
      541 acagagaatc gggtcgttgc gatagtagac tcgcttgacc cggatcaccg gttccttggc
      601 cgcgccatgg gcgtagtaac ccggccactc gccgaacgga ccctccatct gcatttgttc
      661 ctggggcggc ggcacttccc cttcgatggc gatctcggcg tgggcgggaa ttggcaggcc
      721 ggtgagctcg ccgcggatca cgtcgagcgg cgcaccgcgc aggccgccgt aataatcgaa
      781 ctcggacagg ccatagggca tcgattgtcc ggcgactaaa aatagcagtg ggtcttggcc
      841 aaaggtcacg accaccggac agcttttacc ttgctcccaa tatttctcgc gcatgatgcg
      901 cgcatgatgg ccaggcgaga cataaaggcc gagggtgttc ttgtcgtgga tctgcacgcg
      961 atagataccg acgttgaccc atccaccttc acccggttcg ccggtaatga cgcagtcggc
     1021 ggtgccgata tagcggccgc cgtcgagctc atgccagtgc ggcgtcggga aaatcgtcat
     1081 gtcgacgtct gcgccggatt tgacgttgtc gagaatcgga ccgctagaga ctagccgtgg
     1141 cggcaccggt ttcacgccct tgagcagttc tttccatttg gcaatgagtt caatgggccg
     1201 ggcattcggc ggcatgccga ccgtgagact cgaacgcgat ggtgtattga ttaagttcga
     1261 cgcaacgcga aagttgggcg cgtagccggg cacttgatcg aaaagcacca ggggcgaggg
     1321 ttctttgaaa gcgaatagtt cggtgagagc accgatctcc aattcccaat gggcgccatg
     1381 aatgtctttc acttccccga gcgcgcgcgc ggcgtcgata aattcccgta gatcgttgat
     1441 cggcatgttc ttgctttcgt cgaagagatc tcgcaccacc aaggccacga acggcatagg
     1501 taggggcgaa aaatctttcg cccctacatc gtggtgcaat atgtttatta gaagtggcct
     1561 agctcttttg catgtttata gatgccgtcg tgaaacattg cgacgcattc ggcattcgtc
     1621 aggcagcaat attcggggcc ccagccgggg cgataccaag tattaccgtc ctgcaataaa
     1681 cgctcgaaga tctgccgggc cacttcgccg tgggccatga ggccgtccag atgttcgtgc
     1741 aagtcggctt cttcatgcac cgcgaaataa tgcaggtccg cttccttgac atgatatttg
     1801 ccgagcagcg cctcgcgcca gcgcttggcc caatggccga acatctcttc atttaacgac
     1861 cgcgcccacc actcgatcat gctgccttcg taaaccaggc cgcgggcgaa ctcggtcaag
     1921 gcgcggcatt cggcatagac ttggcaatta atcatttcgt cgagggacac gccgaattgt
     1981 tctccctgtt tgatcagtac ttcgatgtgg ccgggaattt tcggattgac caactcgtcg
     2041 ctgattttac cgacgtaggg cgcgagcaag tcacgatggc gcgcaaaaat gccctgatgg
     2101 cgctggtaga tgatcaagtg aaagttgttg acctccaccg gatagctgta ccagttgcgc
     2161 cagaacagtt tcagggtttc gaagggcagc cggccggctt cgagatcgcg catgaacgcg
     2221 ccgccgaaaa ctttctcatc ccatagcgcg tacatgcgct gtttgatcgc gtcggtaaca
     2281 gctttcgcct cggcgggcga atgttttttg ccgatttcca aaactaatgg attttgattc
     2341 atgatcgtcg tgtcgccggt tgttccagca aaatttcctc tcgatcccct ttttcaaacg
     2401 tggtaaatat ccaagctaaa gagcgtggtt atccgcgctc cgaaaccctc tttgaagaag
     2461 agggcaggga aatctatcct ttaaaaccgg cattctccaa gccttccttc aattccgccc
     2521 ggtctttgtc ggtaaatggc ggggtttccc aacgcgggaa acgttccacc ggcagacctc
     2581 tgagcttcat gagctcgcag aaaacaccac ggccatggct gcgaagatat ttgccgacca
     2641 cctgcatgag ccgggtcaat agatcatgct ccgtacgcgc ggccttgaga tcgtcggctt
     2701 tgagcgcttc gtacattttc acgcaagctt cgggaaacgg cgtcgacggc ggatgaatcg
     2761 cgccgcgcac gccgaccgag taaccggaat agagagaaaa aatcccgccg ggaaaaactc
     2821 cggtagtctt cggcaattga tagacgtagc cgagcagctt gtcgtaggcg atgaaagaaa
     2881 ctttgatgcc ggccaatgga ttgggcgcga tggcttcgat aatcttgttg gccttcgccg
     2941 gggtgatttc gacccaggtc gtttcggtat tgtcgtagat aaacatcggc gtgccgggca
     3001 ccgcctgggc gatcgccttg taatgggcgt aaacttccca gtcgtcatgg gggtaatagt
     3061 aaggcggcac gacaccgacg gcgtcgacgc ctaggccaac ggcatgcttg gccaattcga
     3121 tcgaggagag cgtgttggca gtgccgatat gcatgaccac cggcacgcgg cctctgaccc
     3181 ggttgacgat ctgctcagcg actttcttgc gctcgtcgat ttccatgacc atgccctggc
     3241 cgtgggtgcc caggggaaaa aatccatgca cgcccgcgtc catgtagaaa tcaatgatcg
     3301 catcggtcgc cttggtgtcg atggcgccgt catgtttgaa cgccaccggc atgggcatca
     3361 aaatgccttc gatttttcta ttacttgcca taaatgctcc tttcgctgcg cctgaaactt
     3421 attacttatg ctaacccata gcgcacccct ggcgagattt ccaactgaat cgaacggcgg
     3481 caaagcgcca ggggtgtaag tcggttacgg gaaatgttag ggttcgcaaa atatttcttt
     3541 gggaggcaat catggctgat aaaaatcctg tggtattgat gtcaacgagc atgggcgata
     3601 tccgcatcga gctggacgcg gcgaacgcgc cgatcacgac caagaacttc gtcgattatg
     3661 tgaacgccgg ccattacgac ggactgatct ttcatcgagt cattcccggc tttatgatcc
     3721 aaggcggcgg catggacgcg gcgatgcacg agaaggccaa caaagcaccg ataaaaaacg
     3781 aggcggccaa cggcctaaag aacgaactcg gcactctg
//
LOCUS       DASQKS010000415         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2342374, whole genome shotgun sequence.
ACCESSION   DASQKS010000415 DASQKS010000000
VERSION     DASQKS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342374"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2416
                     /locus_tag="VE170_10750"
     CDS             101..2416
                     /locus_tag="VE170_10750"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ15958.1"
                     /translation="MTTEHQPTGLIGASPKRVEDPPLITGKGCYTDDIQLPGMLYLAF
                     QRSPYPHAKILSIDTAKAKTMAGVALVVTGDDLGEKLNLAPSQVLPNMIIPAHPVLAR
                     GAVHCVGVPVAAVVAKTRAQAQDAAQAIEVEYAELPGVSNAEDALKPGAPLAHQELAS
                     NICYTMTKSGGDVDKAFAAADHVFTMHIVSPRQVALAMEPRGIAVNPDPFGKNLTVWL
                     STQGPHRARAEISGVLGFPEHRIHLIAPDVGGGFGSKGPVYREDIVACYVALKLRKPV
                     KWIATRSEDFLTTIQGRDQAMSSEMALSKDGKILGLKVKVVANLGAYLHSSTAGPPQR
                     MMGMACGSYQIKDCRVEIVSAFTNTCPTGPYRGAGRPESVLNIERLLDKAADVLGMDR
                     LAIRRKNFIQPDQFPYTTGTGVEYDSGDYEKPLREALKLSNYEELLRQRDARRKNGEL
                     VGVGFSTFVEPSGGAGFESGSVRVERTGEITVLTGSSSHGQGHETSWAQIAAGLMQTS
                     MDHVTVLHGDTHVSQQGTGTFGSRSAVVGGGALATAAERVIAKGKRIAAHLVEASPED
                     IVQADDGFAVKGVPDKKVSWRQVAAAAYGGRVPQGMEIGLQETAFFDPKREAWGFGTH
                     ISLVSVDRDTGNFNLEKLVMVDDCGVVINPMIVEGQIHGGVAQGLGEALREQMLYSPE
                     GQVQTDTLMNYAIMRAGDMPPMTLGETITPNPFNPLGVKGVGECGTNGAPPSVANALM
                     DALKPLGIDHVDMPYTAPKLWQLIRQAEAKH"
     gene            2514..>3304
                     /locus_tag="VE170_10755"
     CDS             2514..>3304
                     /locus_tag="VE170_10755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15959.1"
                     /translation="MLTGILRSLVALTFILTFSSTSHAQVTPDKIVVSYPSKSITNFP
                     ILETAQRRGFFQKENLAVSMVYMRGGIDIKALITGDADFGTGSTTAVTAYVAGAPLRV
                     VMSMNAFVDQALYAQPKYRNLAQLKGQSIGSLNPGGLVDTLLRRIIAKEGFSPERDFI
                     LLNMGGTPERYAALKSGTLAASMLSAPHSLRAEKDGFIKIAATRDYVDVPGTAFVTHT
                     DKIKKQPNVIKRFMRAGLRAMNYIRDNRTDTVQMIMREFGMDQEIA"
BASE COUNT          706 a         1082 c          940 g          576 t
ORIGIN      
        1 aaacccgtga tagtggctcg tgttcgcaac agatgacagc tgtcgacagt cttgaagaag
       61 gcgaacacgc atcgcgactg cgttattttg agaggtaacc atgactaccg aacatcaacc
      121 cactggactc attggcgcct cacccaagcg cgtcgaggat ccaccgctga tcaccggcaa
      181 gggctgctac accgacgata tccaattgcc gggcatgctc tatctcgctt ttcagcgcag
      241 cccctatccg cacgcgaaaa ttttatccat cgacaccgcc aaggccaaaa ccatggccgg
      301 cgtcgcgctg gtcgtcaccg gcgatgatct cggcgagaag ttgaatctcg cgccgtcgca
      361 ggtgttgccg aacatgataa ttccggccca tcccgtgctc gcccgcggcg ccgtgcattg
      421 cgtcggcgtg ccggtggcag ccgtggtcgc gaaaacccgc gcccaggcgc aagacgccgc
      481 tcaggcgatt gaagtcgaat acgccgagct gcccggcgtc tccaatgccg aggacgcgct
      541 caagcccggc gcgccgttgg cacaccagga actcgctagc aacatctgct acacgatgac
      601 gaaaagcggc ggcgacgtcg acaaagcctt cgccgccgcc gaccatgtct tcaccatgca
      661 tatcgttagc ccgcgccagg tggcgctggc gatggaaccg cgcggtatcg cggtcaatcc
      721 cgacccgttc ggcaagaacc taaccgtctg gctgtcgact caggggcccc atcgcgcccg
      781 cgccgagatt tccggcgtgt tgggctttcc cgagcatcga attcatttga ttgcacccga
      841 tgtcggcggc ggcttcggca gcaaaggacc ggtctaccgc gaagacatcg tcgcctgtta
      901 tgtcgcgttg aagctccgca agccggtcaa gtggatcgcc acccgcagcg aagattttct
      961 caccaccatc cagggacgcg atcaagcgat gagctccgag atggcgctca gcaaagacgg
     1021 caagatcctc ggcttgaaag tcaaagtggt cgccaacctc ggcgcgtatc tgcactccag
     1081 caccgctgga ccgccgcagc gcatgatggg catggcctgt ggcagctatc agatcaaaga
     1141 ctgccgcgtc gagatcgtca gcgccttcac caatacctgc ccgaccggtc cctatcgagg
     1201 cgccggccgg cccgagtcgg tgttgaacat cgagcggctg ctcgacaaag ccgccgacgt
     1261 gctcggcatg gatcgcttgg caatccgccg caagaacttt attcagcccg atcaattccc
     1321 gtacaccacc ggcaccggcg tcgaatacga ttccggcgat tatgaaaagc cgctgcgcga
     1381 agcgttgaaa ctgtccaact atgaagagct gctgcgccag cgcgatgctc ggcgcaaaaa
     1441 cggcgaactg gtcggcgtcg gcttttccac cttcgtcgaa ccgagcggcg gtgccggttt
     1501 cgagagcggc tcggtgcgcg tcgaacgcac cggcgaaatc accgtgctca ccgggtccag
     1561 ttcccacggc caaggccatg aaacttcctg ggcgcagatc gccgccggtc taatgcagac
     1621 cagcatggat cacgtcaccg tgctgcacgg cgacacccat gtgagccagc aaggcaccgg
     1681 cacgttcggc agccgcagcg ccgtggtcgg cggcggcgcg ctcgccaccg ccgccgagcg
     1741 ggtcatcgcc aaaggcaagc gcatcgccgc gcacctggtc gaagcgtcgc cggaagacat
     1801 cgtccaagcc gatgacggct tcgcagtcaa aggcgtgccc gacaaaaaag tcagctggcg
     1861 ccaggtcgcc gccgccgcct acggcggacg agtgccccaa ggcatggaaa tcggcctgca
     1921 agaaaccgct ttcttcgatc ccaagcgcga agcctggggc ttcggcaccc atatctcgct
     1981 ggtcagcgtc gatcgcgaca ccggcaactt caatctggaa aaactcgtca tggtcgacga
     2041 ctgcggcgtg gtcatcaacc cgatgatcgt cgaagggcag attcacggcg gcgtcgccca
     2101 agggctcggt gaagcgttgc gcgagcagat gctctacagc ccggaaggcc aggtgcaaac
     2161 cgacacgttg atgaattacg ccatcatgcg cgccggcgac atgccgccga tgaccctcgg
     2221 cgaaaccatc acacccaacc cgttcaatcc gctgggcgtc aaaggcgtcg gcgaatgcgg
     2281 caccaacggc gctccgccat cggtggccaa cgccctcatg gacgccctca agccgctcgg
     2341 catcgatcac gtcgacatgc cctacaccgc gccgaagttg tggcagctga tccgtcaggc
     2401 ggaggcgaag cactaggcag cagactttaa cgagagtgca agggccgacg cttggcgcgg
     2461 cccttttctt tttctgagtg gttttctata caggcgctgt aaggagaaca aatatgctca
     2521 cgggaattct ccgctcccta gtggcgctta ccttcattct gacgttttca agtacaagtc
     2581 acgcccaggt caccccagac aaaattgtcg tcagctatcc aagcaagtcg atcactaatt
     2641 ttcccatcct tgaaaccgcc cagcggcgcg gcttttttca aaaagaaaat cttgccgtca
     2701 gcatggtcta catgcgcggc ggcatcgaca tcaaagcgct catcaccggc gacgccgact
     2761 tcggcaccgg tagcaccacc gcggtcaccg catacgtcgc cggcgcgccg ctgcgagtgg
     2821 tcatgagcat gaacgctttc gtcgatcagg cgctctacgc acagcccaaa tatcgaaact
     2881 tggcgcagct caaaggccag tccatcggct ccctcaaccc cggcggtttg gtcgacacgc
     2941 tgctgcgcag aatcatcgcc aaggaaggct tctcgccgga gcgtgatttt attttgttga
     3001 acatgggcgg cacgcccgaa cggtatgccg cgctgaagtc gggaaccttg gccgcgtcga
     3061 tgctgagcgc gccgcacagt ctgcgcgccg aaaaagacgg gtttatcaag atcgccgcca
     3121 cccgcgacta tgtcgatgtt cccggcacgg cgttcgtcac ccacaccgat aagatcaaaa
     3181 aacaaccgaa cgtcatcaag cgtttcatgc gcgccggatt gcgcgccatg aactacatcc
     3241 gcgacaaccg caccgacacg gtgcaaatga tcatgcgcga attcggtatg gaccaggaga
     3301 tagc
//
LOCUS       DASQKS010000416         5610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6117611, whole genome shotgun sequence.
ACCESSION   DASQKS010000416 DASQKS010000000
VERSION     DASQKS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6117611"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..753)
                     /locus_tag="VE170_10760"
     CDS             complement(241..753)
                     /locus_tag="VE170_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15960.1"
                     /translation="MPHSKSLRAVGESLHSLEVTSFVLEKSGQGYTVRTHQPIDPAHL
                     LKLYSSEETLEAPNPSSRHAKLFREDGMLHYDASYVSWLGALGKRQRRNRFTAQASST
                     ANLAQLLRTLGRHLDRVEPHDFRIVWSDGAAVSLDYQRPDGSWVQEMLTTAKLRELAL
                     QNRLRRAPRK"
     gene            complement(802..1620)
                     /locus_tag="VE170_10765"
     CDS             complement(802..1620)
                     /locus_tag="VE170_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYJ15961.1"
                     /translation="MTEKFETLLLSRNGGITTIMFNRPEKRNAMSPQLHREMYQVLSE
                     LRYDKQTKVVILTGAGDNFCAGQDLKQYGLEMESQPEWVKDEIREKVRKWRTDLLRAL
                     PQPIIARITGWCLGGALTVTVGCDVAIAADDAKFGLPEVNFGHFPAGETTPILANYLQ
                     SKHGLYYALSGKMMSAQEAERIGLISRVVPRTELDNEVNEFAKCLAEKSPRALRAVKE
                     AWYTSMKASPDVGFTISNFISERIVREQGGRPGLEQFVAKKLRPMSGTMKLEKE"
     gene            1722..2747
                     /locus_tag="VE170_10770"
     CDS             1722..2747
                     /locus_tag="VE170_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ15962.1"
                     /translation="MNNIALAFLIMVLSAGRLSAQGSFFEGKTIRIIVGLPAGDAYDI
                     YARMLATYMGKYIPGNPAVMVQNMPGASSMITANYVYNVAKPDGLTFGSTLPSLYFDQ
                     LLGRKEVKFDWRKFTWLGSFESSNNLIYMRADTPYKTIHDVTKAAEPPKCGSTGTGSP
                     SYFLVKLLNEAIGTKFTVVTGYQGGQEIDLAVERGEAVCRGFTVTTFFAREPFHSWRK
                     RGFVRVLVQTGRKRDPRLADVPTVNELLDEYKVSDGIRRLSGLALASGDFGRPIVASP
                     GIPAERAKMLREAFARTLKDGELLSEAKKKNLEIDPTSGEDLENLAKDVMSANKEAVD
                     RLHNLLR"
     gene            3007..3642
                     /locus_tag="VE170_10775"
     CDS             3007..3642
                     /locus_tag="VE170_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15963.1"
                     /translation="MCGTFQFNQGGKTIVANEASPERSHLILASLSISAPFLACEGQA
                     NAINIRLMVSRYNHARELRTVGQQLAKQNIDIFELRYNDGEYVLICGDPNPPFTDLIH
                     LRYSTFELKSLEFRAIEARSSGFKLVEFTGLAEILRAIGRHVERLDAKLVSISAPDGA
                     LDGSILKIAYETRIGRNRNEDMVADDIASLAMRMYKERAYINGGSREGRSA"
     gene            complement(3639..5141)
                     /locus_tag="VE170_10780"
     CDS             complement(3639..5141)
                     /locus_tag="VE170_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15964.1"
                     /translation="MDEISFVDTTLRDGHQSLWAEGMLTGMILPIAERMDQAGFEAIE
                     LVAPSFFKKACREFKEDIWERIRLVRQRVANTPLRAIRNRYMTGFQVTPALVTQLWLE
                     RLAANGIRELRSSDPSNTAANWAEMVRSANEVGIATIINLTFSESPKHSDAYYAERAR
                     QAAALRPARICIKDPGALLTPDRTRTLVPTVLAAVGAVPVEFHTHCITGLGPMCCYEA
                     IKLGIRSINTAIPPLANGSSNPSLFTVARNARALGYRTAVDEAVLQPVAGHFNIIAKR
                     EGFAPGEPREYDSYHYHHQVPGGMISNFRFQLGKLGMAHRIGEVLEEVARVRRELGYP
                     IMVTPYSQFVGVQAAMNIILGARYQECTDEVIQYALGAWGEDESSAIEANVKDRILDR
                     PRARELKQDQAPELTLQQLRVKLAGAGVSDDELLLRYFAGKEDVERMKGAAPVRPYLS
                     GQQPLVLLIQELSKKKNLGQVVVRRGELTIRLERQGERPTLRSGSAARVG"
     gene            complement(5144..>5610)
                     /locus_tag="VE170_10785"
     CDS             complement(5144..>5610)
                     /locus_tag="VE170_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HYJ15965.1"
                     /translation="ENHFGSDHVALLLASLRRLTGRDLVDRQLPMVEQARRIFYAPFA
                     VLSHNCAADPILNYGNRVAMELFELSWQELIRTPSRLTAEPVRRDERERLLDAVRRHG
                     FIDDYCGVRVSQSGRRFYIETATVWNVMDEAGARYGQAATFSAWKHVDAEIR"
BASE COUNT         1206 a         1682 c         1537 g         1185 t
ORIGIN      
        1 tgagccttga gcaccagcgc cggacgtttc tgatctgccg gcggaaaggg atcggcaaag
       61 atattcgctg gcaacatcat gcgccaaacc ataagcgcaa agagcagtcg agtttttact
      121 gatcgcatgg gcagcgcctc cgccagcttt tcacctgtca gcggcagtgg attcaggttc
      181 gttgtacgtt tgaagcgcgc gagaagtcaa tcgccatacg cggggcgctg ccgggcatgc
      241 ttacttgcgt ggcgcccgcc gcagccgatt ctgtaaggcc agttcgcgca gcttcgcagt
      301 ggtgagcatt tcttgcaccc aactgccgtc aggccgttgg taatcgagag acaccgcggc
      361 gccgtcgctc cagacaatgc gaaaatcatg gggctcgacg cgatcgagat gacggccgag
      421 ggtgcgcaac agctgcgcga gattcgcagt gctactcgcc tgtgctgtaa aacgattgcg
      481 ccgttgacgc ttgccgagag cacccaacca ggaaacatag ctggcgtcgt aatgcagcat
      541 accgtcttcg cgaaacagtt tggcgtgacg gctggaaggg ttcggcgcct ctaaagtttc
      601 ttccgatgag tagagtttga gcagatgcgc gggatcgata ggttgatggg tacgtacggt
      661 gtacccttgc cccgactttt ccaaaacgaa gctcgtcacc tcaagagaat ggagtgactc
      721 accgacggct ctgagagact tgctatgggg catttggatg atcggtatcg cgcgagccga
      781 tgccacctcg gcaacaggga actattcctt ttccagcttc atcgtgcccg acatcgggcg
      841 cagttttttc gcgacgaact gttccagtcc gggccggcca ccctgttcgc gcacgatccg
      901 ctcggaaata aagttggaga tcgtaaagcc gacgtccggc gaggctttca tactcgtgta
      961 ccatgcctct ttaacggctc tcaacgcccg cgggctcttc tcggcgaggc acttggcgaa
     1021 ctcattcact tcgttatcga gttcggtgcg cggcaccacg cgcgaaatca gaccgatccg
     1081 ctcggcttcc tgagcggaca tcatcttgcc cgacaaggcg tagtaaagcc catgcttcga
     1141 ctgcaagtaa ttggccaaga tcggcgtcgt ctcgccggcg gggaaatggc cgaagttgac
     1201 ctccggcagg ccgaacttgg cgtcatcggc ggcgatcgcc acgtcacagc ccacagtgac
     1261 cgtgagcgcg ccgcccaaac accagccggt gatgcgcgcg atgatcggtt gcggcaaggc
     1321 gcgcaggagg tcggtgcgcc acttgcgcac tttctcgcga atttcatcct tcacccactc
     1381 gggctgggac tccatctcca agccgtactg tttcaaatct tgcccggcac agaaattatc
     1441 ccccgcgccg gtgagaatca ccaccttggt ctgcttgtcg tagcgtaatt cgctcaagac
     1501 ctgatacatc tcacgatgca attgcgggct catggcgttg cgtttttccg gacgattaaa
     1561 catgatcgtc gtgatgccgc cgttgcggct caacaataag gtttcaaact tttcggtcat
     1621 ggcctttctc cttgtgaatc agcggaatca atttgacaca gacgagcgca ctcggctagg
     1681 cttcagccgt ctttagccta gcggttacga ggagctgcgc tatgaacaac atcgctctgg
     1741 cgttcctgat aatggtttta tccgctggca ggctgtcagc gcaaggatca tttttcgagg
     1801 gcaagacgat ccgcatcatt gtcggtctgc cggccggcga cgcctacgat atctatgccc
     1861 gcatgttggc gacttacatg ggcaaatata tccccggcaa tcccgcggtg atggtgcaaa
     1921 acatgcctgg cgcatcgagc atgattaccg ccaactatgt ttacaatgtc gcgaagcccg
     1981 acggacttac cttcgggtcg accttgccgt cgctttactt cgatcaactt ctcggccgta
     2041 aggaagtgaa gttcgattgg cgcaaattta cctggttggg cagctttgag agctcgaata
     2101 acttgatcta catgcgcgcg gatactcctt ataaaaccat ccacgatgtg accaaagccg
     2161 cggagccgcc caaatgcggc tccaccggca ccggctcgcc gtcttatttt ctcgtcaagc
     2221 tgcttaacga agcgatcggc accaaattta ccgtcgtcac agggtatcag ggcggtcagg
     2281 aaatcgatct agcagtcgag cgcggcgaag cggtgtgtcg cggtttcacc gtgacgacat
     2341 tctttgcccg cgaaccgttt cacagctggc gcaagcgcgg ttttgtccgt gtcctggttc
     2401 aaaccggcag gaaacgcgat ccgcgcttgg ccgatgtgcc gacggtcaat gagttgctcg
     2461 atgagtacaa agtttcagat ggaatacgcc gattgtcagg cctagcactg gcgtccggcg
     2521 atttcggccg gcccatcgtc gccagccccg gcatccccgc cgaacgcgcg aaaatgctcc
     2581 gtgaggcttt tgccaggaca ctgaaggatg gcgagctact gtccgaagcg aaaaagaaaa
     2641 acctcgagat cgatccgacc agcggcgagg atctcgaaaa tttagccaag gacgtcatgt
     2701 cggcaaataa agaagccgtc gaccggctgc acaacctatt gaggtgaatt ggctgatctc
     2761 gactttaagc cgccggctat tgcaagtgtc gatttgaagt cgacatgtcg tcatttccgc
     2821 aaacgcgaaa tccagcttcc agtcattcac cccaaccgat ttggacgcca gccgccgtcg
     2881 ccgtaacgta attcatcaaa gttcgtcacc ggtcagatct gccgagcgcg cctctggatc
     2941 gcctctctcc cgcgcgcccg attcattcac tgtctcgatc gacggtctcg ttcaccgaga
     3001 aggtgagtgt gcggcacttt ccagttcaat cagggaggaa aaaccatcgt agccaacgag
     3061 gcttcgcccg agcgcagcca cttgatatta gcgagcctat caatttcggc gccatttttg
     3121 gcttgcgagg gccaggcgaa tgcgataaat attcgactca tggtgtcccg ttacaatcac
     3181 gcccgagagc tgcgcaccgt cggccaacag ctcgctaaac aaaacatcga catcttcgag
     3241 ttgcgctaca atgacggcga atacgtcctg atctgcggcg atccgaatcc acctttcacc
     3301 gatttgatcc atctcagata ttcaaccttc gaactcaaat ctttggaatt tcgtgccatc
     3361 gaggctcgaa gctccggatt caagttggtg gagttcacgg gtctggcgga aatcctccgc
     3421 gccatcggcc ggcatgtcga acgactcgac gccaaattag tcagcatatc ggcaccggat
     3481 ggcgcattgg acggcagcat actcaagatc gcctacgaaa cccgcatcgg ccgcaaccgt
     3541 aacgaagaca tggtcgccga cgatatcgcc agtctggcga tgcgcatgta taaagagcga
     3601 gcttatatca acggcggttc tcgcgaaggc cgctccgcct aaccaacccg cgctgcgcta
     3661 cctgatctaa gggtcggccg ctctccctgc cgctccaggc ggatcgtcag ctcgccgcgg
     3721 cgcacgacaa cttgacccaa gtttttcttt ttactgagtt cctgaatcag caacaccagc
     3781 ggctgttgcc cgctcaagta cgggcgcacc ggcgccgcac ctttcatccg ctcgacatct
     3841 tccttaccgg cgaaatagcg cagtaacaat tcatcgtcgg ataccccggc accagccaat
     3901 ttgacgcgaa gctgttgcaa ggtgagctcc ggcgcttgat cctgctttag ctcccgcgct
     3961 cgcggccggt cgagaatcct gtctttgaca tttgcttcga tggctgagct ctcgtcctca
     4021 ccccaggctc ccaaggcgta ctgaatcact tcgtcggtgc attcttggta acgcgcgccg
     4081 agaatgatat tcatcgccgc ctgcacgccg acgaattgcg aatacggcgt caccataatc
     4141 gggtaaccca actcccggcg cacccgagcg acttcctcca acacttcacc aatgcgatga
     4201 gccatgccga gcttgcccag ctgaaaacga aaattggaaa tcatcccgcc gggaacctgg
     4261 tgatggtaat gataactatc gtactcgcgc ggctcgccgg gagcgaaccc ttcgcgcttt
     4321 gcgataatat tgaaatggcc tgccaccggc tggagcaccg cttcatcaac ggcggtgcga
     4381 tatcccaatg ctcgggcatt gcgcgcgacg gtaaaaagcg acggattcga tgaaccgttg
     4441 gcgagcggtg ggatagccgt attgatcgaa cggatgccta gcttgatcgc ctcatagcaa
     4501 cacataggcc ccagcccggt gatgcaatgg gtatgaaact cgaccgggac agcgccgaca
     4561 gcggccagca ccgtagggac taaagttcgg gttcgatcgg gggtcaacag cgcgcccggg
     4621 tctttgatgc aaatgcgtgc cggccgcagc gccgccgcct gccgggcgcg ctcggcgtaa
     4681 taagcatccg aatgtttggg cgactcggaa aaagtgagat tgataatcgt cgctataccc
     4741 acttcgttgg ccgagcgcac catctcagcc caattggctg cggtattgga gggatcggag
     4801 ctgcgcagct cgcgaatgcc attggcggcg agccgctcca gccacagctg ggtgaccaag
     4861 gccggcgtca cttgaaagcc cgtcatgtaa cgattgcgaa tggcgcgtag cggcgtgtta
     4921 gcgacgcgct ggcgcaccag tcgaatgcgc tcccagatgt cttccttgaa ctcccggcaa
     4981 gcctttttga aaaacgacgg cgccactagt tcgatggctt cgaaaccagc ctgatccatc
     5041 cgctcggcga tcggcagaat catgccggtc agcattcctt cagcccatag gctctggtgt
     5101 ccatcgcgca gcgtcgtatc gacaaagcta atctcatcca tgatcatctt atttcggcat
     5161 cgacatgctt ccacgcgcta aacgtcgcag cctgtccata acgcgcaccc gcctcatcca
     5221 tcacattcca caccgtggcg gtttcgatat agaagcgacg cccgctttgg gagactcgaa
     5281 caccgcaata atcatcgatg aagccatgcc gcctcactgc gtcgagcaag cgctcccgct
     5341 cgtcgcggcg caccggctcc gcggttaagc gcgagggtgt acggatcaac tcctgccagc
     5401 tcagttcgaa gagctccatg gcgacacgat tgccatagtt cagaatcgga tcggcggcgc
     5461 agttatgcga cagaactgca aacggcgcat aaaatatccg gcgcgcctgc tcgaccatcg
     5521 gcagttgccg gtcgactagg tcgcgcccgg tcaagcgccg gaggctagcg agcaacaacg
     5581 cgacatggtc gctgccaaag tggttttctt
//
LOCUS       DASQKS010000417         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1489865, whole genome shotgun sequence.
ACCESSION   DASQKS010000417 DASQKS010000000
VERSION     DASQKS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489865"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1957)
                     /gene="treZ"
                     /locus_tag="VE170_10790"
     CDS             complement(32..1957)
                     /gene="treZ"
                     /locus_tag="VE170_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HYJ15966.1"
                     /translation="MTRPRHRDRPVEAAATTQWATDEKTPAQNHDETLGAVILSGTTC
                     RFRVWAPTRSTVNVHLLGPNRIVPMRQSGRGYYEANVEHVHPGDLYLYQLAPDIERAD
                     PASRFQPKGVHGPSQVIDCKIRWDEKGWSGRPLKDYIIYEIHVGTFSPTGTFDAIIPY
                     IEYIIQLGVTAIELMPVAQFPGARNWGYDGVFPFAAQNSYGGPPALKKLINACHQRGL
                     AVILDVVYNHLGPEGNYLADFGPYFSDNDNTPWGPSVNFDGEFSDEVRHYFIQNALYW
                     IGEFHIDALRLDVGHTMVDDAPVTFLEELSAQVHRTAERLNRQIFLIAESADNDARLI
                     RSHEQGGCGLDAQWNDDFHHCLRTLLTGERAGYYEDYGEFAQLIKAYRDGFVYSGEYS
                     RFHHRRHGNVSHDIPANHLVVFAQNHDQVGNRMDGDRLSQTVSIEELKLAAGLVILSP
                     FIPLLFMGEEYGETAPFPYFISHSDAELAEAVRRGRSDALAAFGWQGELPDPRDARMF
                     LRAKLNHELRNADQHLVSLKFYRELIRLRKKVAALNSLKADNMEVEGFEKERVIVVHR
                     WQDEDLAFLIANLGAAQACVSLSVAGGHWRNVVDSADETWRGKGSLLPRDFCASAENQ
                     LALMPRSVALFVREKVA"
     gene            complement(1957..3957)
                     /gene="glgB"
                     /locus_tag="VE170_10795"
     CDS             complement(1957..3957)
                     /gene="glgB"
                     /locus_tag="VE170_10795"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYJ15967.1"
                     /translation="MSTESLPNNSPTPGVTQQGAARRLGYGFSLLTDDDLFLFNQGSH
                     SRAYDKLGARRLIRDGVEGTYFAIWAPAAEKVFVTGTFNNWDKQSDPLQPRGNSGIWE
                     GFVAGVNKGALYKYVIHSRFFGQRLEKADPFSIFNEIPPRTASIVWDLEYLWNDQAWM
                     ADRRSRNSLAAPISIYEVHLGSWRRIGAEGNRSLSYREMAPLLADYVRHTGFTHVEFL
                     PLMDHPFFGSWGYQITGYFAPSANYGTPQDLMFLIETLHQNNIGVILDWVPSHFPGDA
                     HGLAYFDGTHLFEHADPRQGVQPEWNSNIFNYGRHEVRSFLISNALFWLDRYHADGLR
                     VDAVASMLYLDYARRDGEWVPNRHGGRENLDAIDFLKQFNQTVYENYPDVQTFAEEST
                     SWPLVSRPVHAGGLGFGLKWDMGWMHDTLEYFSQDPIYRKYHHHRLTFRMLYAFHENF
                     VLPISHDEVVYGKRSVLNKMPGDDWQKFANLRLLFGYMYAQPGKKLLFMGCEFGQRRE
                     WTHDQSLDWELLETPLAAGVQKWVGDLNRCYRDHGALHELDFDPAGFEWIDCNDRDAS
                     VVSLSRKGRDDAASILIVCNFTPLVRGNYRVGAPRGGTWRELLNSDAKYYGGSGVGNL
                     GKIEAGPVGCQGQRYSLYLTLPPLSVLFLTDLDGRHRSGGSS"
     gene            complement(4683..>5210)
                     /locus_tag="VE170_10800"
     CDS             complement(4683..>5210)
                     /locus_tag="VE170_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ15968.1"
                     /translation="CERLVQELLEFGRPKTAEFALTDIEQIVRRTVDLVQPHATKNHV
                     ETLMHIEDRLPPIHADAQQLQQVVLNLCLNAVDAMPNGGTLTVGASFDGANQVTVTVA
                     DTGIGIDAEILPRIFQPFFTSKKRRGLGLGLPICDRIVKSHSGKIDVSSAPGRGTIFK
                     IGLPLKPCAEDKPLQ"
BASE COUNT         1165 a         1462 c         1457 g         1126 t
ORIGIN      
        1 caggttttca tggtcctgac tctgctcgac cttacgcaac tttttcacga acgaacaggg
       61 ccaccgaccg tggcatcaag gctaattgat tttccgccga ggcgcaaaag tcccgcggaa
      121 gcaggcttcc tttaccgcgc caggtttcgt ccgcggaatc gacgacgtta cgccaatggc
      181 cgccggcaac ggacaacgat acgcaggctt gcgcagcgcc gagattggcg atcaaaaatg
      241 ccaggtcttc gtcttgccac cggtgcacga caattacccg ctctttttcg aaaccttcca
      301 cctccatgtt gtccgccttc agcgaattga gagcggcaac ttttttcctg agacggatca
      361 gctcgcgata gaatttaagt gagactagat gctgatcggc gttgcgaagc tcatgattga
      421 gcttggcgcg caaaaacatc ctggcgtccc gcggatcggg gagttccccc tgccagccga
      481 aagcggcgag cgcatcgctc cggccgcgac ggaccgcctc tgcaagctcg gcatccgagt
      541 ggcttatgaa ataggggaat ggcgccgttt caccgtactc ttcgcccatg aacagcaggg
      601 gaatgaacgg tgaaagaatg accagccccg ctgccagctt taactcctcg atcgacaccg
      661 tttggctgag ccggtcgccg tccatgcgat tgcccacctg gtcgtggttc tgcgcgaata
      721 cgacaaggtg attggccggg atatcgtgcg aaacattgcc atgacggcgg tggtgaaacc
      781 gggaatattc gccggaataa acaaagccat cgcgataggc tttgataagc tgggcgaact
      841 cgccataatc ttcgtagtaa ccggcgcgct cgccggtgag aagcgtgcgc aaacagtgat
      901 ggaaatcgtc attccactgt gcgtccagac cgcaaccgcc ctgctcatgc gagcggatca
      961 gtcgcgcatc gttgtcggcg ctttccgcga tcagaaaaat ctgccgattg agccgttccg
     1021 cggtgcgatg gacttgtgcg ctgagttctt cgagaaaagt caccggggca tcgtcgacca
     1081 tcgtgtgtcc gacgtcgagg cgcagggcat cgatgtggaa ttcaccgatc caatacaaag
     1141 cattctgaat gaagtagtgc cttacttcat cgctgaactc gccgtcgaaa ttcaccgatg
     1201 gtccccatgg ggtattgtcg ttatcggaaa aataggggcc gaagtcggcg agataattcc
     1261 cctccggtcc aagatggtta taaactacat cgagaatcac cgctaggccg cgctggtgac
     1321 aggcgttgat taatttcttt aacgctggcg gtccgccgta ggagttctgc gcggcaaacg
     1381 gaaacacccc gtcgtagccc cagttgcgcg cgccgggaaa ttgcgccacc ggcatgagct
     1441 cgatggccgt gacgccgagc tggattatgt attcgatgta aggaatgatc gcatcgaaag
     1501 ttcctgtggg agagaaagtg ccgacgtgaa tctcataaat gatgtaatct ttaagcggcc
     1561 ggccggacca gcccttctcg tcccaacgaa tcttgcagtc gatcacctgc gacgggccgt
     1621 gaacaccctt gggctgaaag cgcgacgccg ggtcggcgcg ctcgatgtcg ggtgctagtt
     1681 gataaagata aagatcgccg ggatgcacgt gctccacgtt tgcttcgtaa tagccgcgtc
     1741 cggactgacg catcggcacg atgcgattgg gtcctagcag gtgcacgttg accgttgacc
     1801 gggtcggcgc ccacactcga aatcggcagg tcgtgcccga caagataacc gcgccgagtg
     1861 tttcatcatg gttttgcgcc ggggtttttt catccgtcgc ccattgcgtc gtcgctgcgg
     1921 cttcgacggg tctgtcacgg tgtcgtggtc ttgtcatcac gaagatcctc cagagcggtg
     1981 ccgcccatct agatcagtga gaaacagcac cgacaacggc ggtaaagtca ggtacagaga
     2041 gtagcgctgg ccctggcagc ccaccggccc tgcctctatt ttgccgaggt tgcccacgcc
     2101 gctgccgcca taatatttgg cgtcgctatt aagtaactct ctccaggttc cgccgcgtgg
     2161 cgcgccgacg cgatagttgc cgcgaaccag cggtgtgaaa ttacacacga tcaagatcga
     2221 cgcggcgtcg tcccgccctt tgcgcgacaa gctcacgacg ctggcatcgc ggtcattaca
     2281 gtcgatccat tcgaatcctg ccggatcgaa atccagttca tggagcgccc cgtggtcgcg
     2341 atagcaccgg ttcaaatcgc cgacccattt ttgcacgccg gcggcaagcg gcgtttccag
     2401 tagttcccag tcaagacttt gatcatgagt ccactcgcgc cgttggccaa actcacagcc
     2461 catgaacagc agtttttttc ccggctgcgc gtacatgtag ccgaacagca gtcggagatt
     2521 ggcaaatttt tgccagtcat cacccggcat tttatttaag accgaacgtt tgccgtaaac
     2581 cacctcgtcg tgggaaatgg gcaggacaaa attctcatga aaagcataaa gcattctaaa
     2641 tgtgagacgg tgatgatgat acttgcggta gatcggatcc tgggagaagt actccagggt
     2701 gtcatgcatc caacccatgt cccacttgag tccgaacccc aatccaccgg catggacggg
     2761 acgtgacacc aacggccagg atgtggactc ctcggcgaac gtctggacgt cgggataatt
     2821 ctcgtagacg gtctggttga actgcttgag aaaatcgatg gcatcgagat tttctctacc
     2881 gccgtggcgg ttaggcaccc attcgccgtc acggcgcgca tagtcgaggt aaagcatcga
     2941 tgccacagcg tcgacgcgca atccgtcggc gtgataacga tcgagccaaa agagtgcatt
     3001 gctgatcaga aagctgcgca cttcatggcg accgtaatta aaaatattac tgttccactc
     3061 gggctggacg ccttggcgcg ggtcggcatg ctcaaaaaga tgcgtgccgt cgaaataagc
     3121 gaggccgtgc gcgtcgcccg ggaaatgcga cggcacccaa tcgaggatga cgccgatgtt
     3181 gttttgatgc agtgtctcga tgagaaacat cagatcctgc ggcgtgccgt aatttgccga
     3241 cggggcgaaa tagccggtga tttgatagcc ccaggaaccg aaaaaggggt ggtccatcaa
     3301 gggcaggaat tccacgtggg tgaaacccgt gtgccgcaca taatcggcga ggagcggcgc
     3361 catctcgcgg taggaaagcg agcgattgcc ttccgcaccg atgcgccgcc aagagcccaa
     3421 gtgtacttca tagattgaaa tcggggcagc gagcgagttg cgtgatctgc ggtcggccat
     3481 ccacgcctgg tcgttccaca aatactccaa gtcccagaca atcgaagcgg ttcgaggtgg
     3541 aatctcgttg aagatggaaa atggatccgc tttttccagc cgctgtccaa aaaatcggga
     3601 atggatcaca tacttgtaaa gcgcgccttt gttgacaccg gcaacaaagc cctcccagat
     3661 ccctgaattt cctctcggct gaagcggatc actttgcttg tcccaattgt taaacgtgcc
     3721 ggtcacgaaa accttttcgg cagccggcgc ccatatagcg aaataggttc cctcgacgcc
     3781 gtcacggatt aaccgacggg cgccgagttt atcgtacgcg cgcgaatggc ttccctggtt
     3841 gaagagaaac aaatcatcgt cggtaagcag gctgaagccg tagcccagcc gccgcgctgc
     3901 gccttgctgc gttacccctg gggtggggga gttgttcggt aaactttcgg tgctcatggc
     3961 tctctcataa ccccgtcgaa tactgccggg cccgcccgtc gccacgacac gcgtggctcc
     4021 cgaccgggtc agatcgccgc gctcgacgac gacaagacca tcctcgtaaa ctaaataact
     4081 ttcgatcccg tgcgcatctg agcgggcagt gttttgacga aaccatgctc aaaaatccag
     4141 aaaggtgccg gcccgccggc aactgatcaa tgacggattg gcttgaatcg ggtgagaaca
     4201 taaattcggc gcataccggt attgaaaaaa ttggcaggaa caaagtcgat agtaggtcct
     4261 tgcgactgca ggaagcggcc ggtttggtgg cgcgctctta ggtcagtgga aaaagccgtt
     4321 aacctagccc gtccgccacg acaatgtcga caacattttc gttgtttgtc ttttacaaga
     4381 ttttgttgtc tgcgagcatt acgacgtcca ttcactcgat tccgcgcgct ccagacttaa
     4441 tcatagtcct ctccagtaat tcagaacgag gtaatgcgta gcgcaaaaaa gggaccagcg
     4501 cctcgtctaa tccaagtggt ttaatttaaa gccagagatg cgattcgcgg ccggcgtgcg
     4561 tggaggaaat tttctgcctt gcaggagtat ttcatacacg gccgagaatt cttcccgatc
     4621 gatgacggcg aggttgaagc agcgcacaac ccgtgtgacg gagcgaaaat ttatcagtcg
     4681 ccttactgca gcggtttgtc ctcggcgcaa ggcttcaaag gcagccctat tttgaaaatg
     4741 gttccacggc cgggggcgct cgacacgtcg atttttccgc tatgagactt tacgatgcgg
     4801 tcgcagatcg gcagacccaa ccccaagccg cggcgcttct tggaggtaaa aaacggctgg
     4861 aagattctcg gcaagatttc ggcgtcgatg ccgatcccgg tatcggccac cgtaaccgtc
     4921 acttgattcg ctccgtcgaa gcttgccccg accgtcaagg ttccaccgtt tggcatcgcg
     4981 tctaccgcat tcaagcaaag attcaacacc acttgctgca gctgttgcgc gtccgcgtgg
     5041 atagggggta atcgatcctc gatgtgcatc agcgtttcga catggttttt cgttgcgtgg
     5101 ggctgcacca gatcgacagt cctacgaacg atctgttcga tatcggtcag ggcgaattcg
     5161 gccgtcttgg gccggccaaa ttccaatagt tcctgcacca gccgttcaca
//
LOCUS       DASQKS010000418         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5675075, whole genome shotgun sequence.
ACCESSION   DASQKS010000418 DASQKS010000000
VERSION     DASQKS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5675075"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VE170_10805"
     CDS             complement(<1..199)
                     /locus_tag="VE170_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15969.1"
                     /translation="MARSHVVRSFLSVVLALHILFSAAEAKAADKLVGFQSARTMSQA
                     MPWIAEEVGLFRKYDLDFQLVY"
     gene            434..1384
                     /locus_tag="VE170_10810"
     CDS             434..1384
                     /locus_tag="VE170_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ15970.1"
                     /translation="MKIVVYGPEHRVGALAGDRNLIDLNRASAQLPARLLSFIEAGKP
                     ALDEAKRVIDKFANAAPADDGIVQALAGVQLHAPWPERRIACVGGNYAKHLLGMWANR
                     LGRTDITVEEVAAEAKKAGQWGFWKVPADVAGPNGTVPFPRRVTYFDYEGEVAIVIGK
                     RGKNIPATKIDEFVWGVTLFHDWSIRDGGGTDRAVSYNLQKNFDGAASMGPCIVVGET
                     GQQDVDVETRVNGVVRQSYSTSEMIWNFGEVLEYLSQDFTFVPGDVIAGGTSAGTAAD
                     KSRRSPDGKRPLDLFLKVGDLVEVSSSKIGALSNRIVAAD"
     gene            1477..2643
                     /locus_tag="VE170_10815"
     CDS             1477..2643
                     /locus_tag="VE170_10815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ15971.1"
                     /translation="MDFNLPEELQILRDTVRRFVDKELIPLEREYRHDSEGPMPDHLL
                     KPLQEKTKAMGLWMLDVPAEYGGAGLGLFPRCIIHEEIARAASLPFRSLELFGPEVRP
                     VLFYCSDEQKERFLKPVLAGEMKFCFAQTEPDAGSDPANMRTRAVRDGDDFIINGTKR
                     FITAAGRADYVQLMAVTDPEKKARGGISCFAVDLKSPGVNLERQWPAMMGEAPWQLYF
                     ENVRVPANNVIGEIGGGFSIAQKWIGEGRIRGHGARPVGIASRALDMMIDYSKVRTTF
                     GKPLSERQAIQFMIADSAMEIHSVRNMVYECAWRVDRGEDVRDLSYMVKVMATEMASR
                     VVDRSIQVHGGLGLMKELPLEWWYRQIRSTRITEGANEVLRWRLGQNIIRAHKD"
     gene            complement(2805..2951)
                     /locus_tag="VE170_10820"
     CDS             complement(2805..2951)
                     /locus_tag="VE170_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15972.1"
                     /translation="MIRATVMADAPMGWRFLYFLPVGGSSGAKEITSQPPDVVERVKK
                     ILEN"
     gene            complement(2973..3959)
                     /locus_tag="VE170_10825"
     CDS             complement(2973..3959)
                     /locus_tag="VE170_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15973.1"
                     /translation="MTVRLRVAIVFICAVWGFDMGLAQERPKIYLGATSKTLGYSPLW
                     VAAKKGFFEQQGLDVQLVLLRGVPMTVQALAANSLHFGSGGPEPYIEASERGLDFTVI
                     GGIINGIAQFLVAGKNYRTYEDLRGATFGTSSLTGGIVTAMREALKLKGLEYPRDYKL
                     LIIAGGSSANLAALQAGQIAATTMAAPLNYAAQEAGFNILGKLSDGIPHFQTNAFVVR
                     RAWAEKNRPITVRFMKAHVLAMRWLFENKDAAVEFLSQEMKLKPLHARLGWEFYTQNR
                     HWPPDGDVTMEGMKNNIRIYAELTGLKGPLPDPVKYVDRSYLTDALKELERR"
BASE COUNT          999 a         1286 c         1238 g          888 t
ORIGIN      
        1 tatataccaa ttggaagtct aaatcatatt tacgaaacaa cccaacttcc tctgcgatcc
       61 acggcatcgc ctgggacatc gtccgggccg actgaaagcc gacgagtttg tcggccgcct
      121 ttgcttcggc ggcgctaaac agaatatgta atgcgagaac cacgcttaga aacgaccgaa
      181 caacatgcga tcttgccata ggtgcgacct cctgcgactt ttccgtctca tcgagcgatg
      241 cgccaagccc gacgacagta tcaaaaagca cggtttccga ctacagcgac tcgatgtcat
      301 ttctccgagt cccgtcgcgc ggtgaaatag ccaaatcaac gcttccgtcc ggcacggcga
      361 aaaccgtcca ggccttgacc gcaagcgcat ggcgatgtta cgcaatcatt catagaatac
      421 tgcggaggca aggatgaaaa ttgttgtcta tggaccggag caccgcgtcg gggcattagc
      481 cggtgatcgt aatctaatcg atttgaatcg ggcgtcggcg cagctaccgg cacgcctgtt
      541 gagcttcatc gaagccggca aaccagccct cgacgaggcc aagcgggtca tcgacaaatt
      601 tgccaatgcc gcccctgccg acgacggcat cgttcaggct ctcgctggcg ttcagttgca
      661 cgctccctgg ccggaacggc gcattgcttg cgtcggcggc aactacgcca aacatctgct
      721 cggcatgtgg gccaaccgcc tgggcaggac cgacatcact gtcgaggagg tcgcggcgga
      781 agccaagaaa gctggccaat ggggattctg gaaagtgccg gccgacgtcg ccggccccaa
      841 cgggaccgtt ccctttcccc ggcgggttac ctacttcgat tatgaaggcg aagtggcgat
      901 cgtcatcggt aaacgcggca agaatattcc ggcgaccaaa atcgacgaat tcgtttgggg
      961 tgtcactttg ttccacgatt ggtcgatccg cgacggcggc ggcaccgata gagccgtcag
     1021 ttacaacctg caaaagaatt tcgatggcgc cgcatccatg ggaccgtgca tcgtcgttgg
     1081 cgaaaccggc caacaagatg tcgacgtcga aacccgcgtc aatggcgtgg tgcgccagag
     1141 ttacagcacc agcgagatga tttggaattt cggcgaggta ttggaatatc tgtcccagga
     1201 ctttaccttc gtgccgggcg atgtcatcgc cggcggcact tcggccggca cggcggcgga
     1261 taaatctcgc cgcagccccg atggcaagcg ccccctcgat ttgtttctca aagtcggcga
     1321 cctcgtcgaa gtgtcgtcgt cgaagattgg cgcgctatcg aacaggatcg tcgccgctga
     1381 ttagccagcg ccttatttta tctcggcgcg acaatccttg ccccgacaat cgccgcaagg
     1441 cttaggtgac gcaaatattt tacaaaggaa tcgttcatgg acttcaatct gccggaggag
     1501 ctacaaatcc tcagagacac cgtgcgacgc tttgtcgaca aggagctgat cccgctggag
     1561 cgcgagtacc gccacgacag cgaaggtccg atgcccgatc atttactcaa accgttgcag
     1621 gaaaagacca aggccatggg tttgtggatg ctcgacgtgc cggcggaata cggcggcgcc
     1681 ggtctcggcc ttttcccgcg ctgcatcatt catgaagaaa tagcccgcgc ggcatcactg
     1741 cccttccgca gcctcgaact gttcggaccg gaagtgcggc cggtgctatt ttactgcagc
     1801 gatgagcaga aagaacgctt tctcaaaccg gtgttggctg gcgagatgaa attttgcttc
     1861 gcccaaaccg aacccgatgc cggcagcgac ccggccaaca tgcgcacccg cgccgtgcgc
     1921 gacggcgatg actttatcat caacggcacc aagcgcttca tcaccgccgc cggtcgggct
     1981 gactacgtcc aacttatggc ggtgaccgac ccggaaaaga aagcgcgcgg cggcatcagc
     2041 tgctttgccg tggatttgaa aagtcccggc gtcaatctcg agcgccaatg gccggcgatg
     2101 atgggtgaag cgccctggca attgtatttc gaaaacgtcc gcgtgccggc gaacaatgtc
     2161 attggcgaaa ttggcggcgg gttttccatc gcgcaaaaat ggatcggcga agggcgcatc
     2221 cgcggccatg gagcccgccc ggtgggcatc gcctcgcgcg ccctcgacat gatgatcgac
     2281 tactccaaag tgcgcaccac cttcggcaag ccgctctccg agcggcaagc catccagttc
     2341 atgatcgccg actcggccat ggaaattcac tcggtgcgca acatggtcta cgaatgcgcg
     2401 tggcgcgtcg atcggggcga ggacgtccgc gacctttcgt acatggttaa agtcatggcg
     2461 acggaaatgg ccagccgtgt ggtcgaccgc tcgattcaag tccacggcgg tttgggttta
     2521 atgaaagagt tgccgctgga atggtggtat cggcagatcc gcagcacgcg catcaccgag
     2581 ggcgccaacg aagtcctgcg ctggcgccta gggcaaaata ttatccgcgc ccataaagat
     2641 tagctataaa aataacctcg ggagttccac cacgaaagca cgacggacac gaggatttgc
     2701 gtaagaaagc tgcaaacttc gtgatcttcg tgctctcgtg gtaaggcaat tcgctccagc
     2761 aagtctgcca agttctcatc aacccgcgat tgaatacagc aaaatcaatt ttccaaaatc
     2821 tttttcaccc gttcgacgac atccggcggc tggctggtga tttccttggc gccagaacta
     2881 ccacccactg ggaggaagta caaaaaccgc catcccatcg gcgcgtcggc catgacggtt
     2941 gcacgaatca agaatcgttt cgagcgcact tgctaacgcc gttcgagttc cttcagcgca
     3001 tcggttaagt aactgcgatc gacgtacttg acggggtccg gcagcggccc tttgagaccg
     3061 gttaactcgg cataaatgcg aatgttgttt ttcatgcctt ccatggtaac gtcgccatcc
     3121 gggggccagt ggcgattttg cgtataaaac tcccaaccga gccgagcatg aagcggtttt
     3181 agtttcattt cctgggacag aaactccacg gcggcgtctt tgttctcgaa caaccagcgc
     3241 atcgccagca catgggcttt catgaagcgc accgtaatcg gacgattctt ttccgcccaa
     3301 gcgcggcgca cgacaaatgc attggtctga aaatgcggga tgccgtcgct aagcttgccg
     3361 aggatattga acccggcctc ttgcgcggcg taattcaacg gggcagccat cgtcgtcgcg
     3421 gcgatctgtc ccgcctgcag cgcggcgagg ttggccgaag agccaccggc gatgatcaac
     3481 agtttgtaat cgcgcggata ttccaaccct ttgagtttga gagcttcgcg catggccgtg
     3541 acgatgccgc cggtgagcga tgacgtgccg aaggtcgcac cgcgcaaatc ttcgtaggtt
     3601 ctgtaattct tgcccgcaac caagaactgc gcgatgccgt tgatgatgcc gccaatcacg
     3661 gtgaagtcga gaccgcgctc cgaggcctcg atgtaaggct ccggccctcc ggagccgaag
     3721 tgcagcgagt tggctgccag cgcctgcacg gtcatcggca cgccgcgcaa gagaaccagt
     3781 tgcacgtcca aaccttgctg ctcgaaaaac ccttttttcg ccgctaccca gagcgggctg
     3841 tagcctagag tcttgctcgt ggcgccaaga taaattttcg gccgctcctg cgccaagccc
     3901 atgtcgaaac cccagacggc gcaaataaaa acgatcgcaa cgcgcagtcg aacggtcatg
     3961 attcctccca ggccgacgat ctcgaaatca aattgtcgcg tgaccgagct ttacaacgag
     4021 gctgattgag ctttcaagag atgacccgga aaatcaccag cttaccgaaa gtgcctattc
     4081 atacgcctgg ttcaatcgct tcgctgtggc tccacagcgc cggacaattt ttctagctgc
     4141 aagtaattat tacgcgagca aaccgacagc ggcggcgtgg cagcatggat atttcaacca
     4201 gttacggaca gaccagactg gcatctcggt tgctctactg gtctgccaac tgacggagca
     4261 aagatgactg gaggcataat gaacaagatc aagaaaatga ccatgggatt tctaatgcta
     4321 accgcgatca ccgcggccgg ctgcgccggt ggttcgttaa ccagcagaga aaaaggcgca
     4381 ggcattggcg ctcttggcgg ggctgcaggc g
//
LOCUS       DASQKS010000419         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3168966, whole genome shotgun sequence.
ACCESSION   DASQKS010000419 DASQKS010000000
VERSION     DASQKS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3168966"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..832
                     /locus_tag="VE170_10830"
     CDS             17..832
                     /locus_tag="VE170_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ15974.1"
                     /translation="MSGRILEVNGRKVWLLENGSGAPLLYLHGFADVHAVKESWLPFH
                     EQLAKSARVIAPAHPGCAQSDENKDVDMIEDVVFHYLEVLDALKLDKFDLVGHCLGGW
                     IAAEIAVRHPEKIRNLVLIGASGLFVEGALIGDIFMNAQPEFGESYATLREMLFANAQ
                     QSNALDMFPDGKGEIEDEVRRYQTLRLSSRIGFKPPYFYNRPLRNRLHRITSPALVIW
                     GEKDGFVPRSHGETYAKLIPNSGDLRIIAAAGHSAHVEKAEETAKMVTEFLAN"
     gene            complement(949..1452)
                     /locus_tag="VE170_10835"
     CDS             complement(949..1452)
                     /locus_tag="VE170_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15975.1"
                     /translation="MKLLTLAFSIIHASMAALFACAALMLITIATKAAWIAFAGSLGR
                     AAAQEIIEAVGLLAAAVVALQIAQTIIEEEVVRDAHISGPTRVRRFLSRFLVVVVVAI
                     AIEGLVATFRAAHEDSAQLIYAASILVAAGVLLAAWGIFIRLNRYAEELEPEAMAEAK
                     REDRKLE"
     gene            complement(1679..2749)
                     /locus_tag="VE170_10840"
     CDS             complement(1679..2749)
                     /locus_tag="VE170_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15976.1"
                     /translation="MKSLSRLGFCGFVHGWLIGLAALLLASEAFAQADFYKGKTIKVV
                     RGGGPGGSGEFQTRALVKFLEKYIPGKPHIMVEFIEGAAGRKAANVIYSTTKPDGLTI
                     GSIGAGLVVGPILGLPGSAYDLDKFVYLGSTDGGDPYVFYTKADAGLDSLAKLRAASG
                     VRFGAHSVGHPVYVTGRIIAYLLGLKDPKFVTGFTGPEIYIAMDRGEIDAHATGAARF
                     VIEKPEWIEKKQIHLHATLTVPRGRHHPSFAQLPEFGSFAKTDTERKLLDMFRAFQYP
                     RWPYIFPPGTPKDRVQIIRDAMNKSFADPEFPVEFKKLMGDGPAPLSGEELERAIKEL
                     PRDKEVVQLYQKVAGADPLPAR"
     gene            2933..4150
                     /locus_tag="VE170_10845"
     CDS             2933..4150
                     /locus_tag="VE170_10845"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYJ15977.1"
                     /translation="MIKKVKVDQLKPGVFIADFNCGWLHHPFLTDRTKLETDKQIQKV
                     IKYGIRELYIDTDKGLDIDDAPTQREADQEIQSEFSRLNAPAAGPNRGDGFKAELARA
                     HRLVSEAKLTTKKLMHDIRLGKRMAMNQVESTVDKMTDSILRNKDALVSLLRIKEVDE
                     YTYVHSISVSALCISFAHGLGLDATKIKQIGIGGLLHDIGKMKVPLEILNKPGPLSEK
                     EFEVMKRHVKDGEAALQQYSNLDQECTCVTSHHHERLDGTGYPDGLKGNEISEFGQIA
                     AIVDIYDALSSERCYKNAMPPTDALKKLFEWSQSYLNRELVEKFIAHLGIYPIGTLVR
                     LENLLLAVVIDHGEKNLLHPVVRVVYDMKKASRLEPFDIVLEGQINAGNPYGIVGCES
                     PDKWQIKPVDYLY"
BASE COUNT         1038 a         1208 c         1166 g          927 t
ORIGIN      
        1 cacaggcggt gcagtaatga gcggtcgcat cttggaagtc aacggacgaa aagtttggct
       61 tttggaaaac ggcagcggcg cgccgctgct ttacttgcat ggcttcgccg acgtgcatgc
      121 ggtcaaggag agctggttgc cgttccacga acagctggcg aaaagcgcga gggtcatcgc
      181 cccggcccat cccggctgcg cccagagcga tgagaataaa gacgtcgata tgatcgaaga
      241 tgtggtcttc cattatctcg aagttctcga cgccttgaag ctcgacaagt tcgaccttgt
      301 cggccactgc ctgggcggct ggatcgccgc cgagatcgcc gtgcgccatc cggaaaaaat
      361 tagaaacctg gttttaatcg gcgcctccgg cctgtttgtc gaaggtgcgc tcatcggcga
      421 catattcatg aacgcccagc ctgagttcgg cgaaagctac gccactctgc gcgagatgct
      481 tttcgccaac gcgcagcagt cgaatgctct ggacatgttc cctgacggca aaggcgagat
      541 cgaagacgag gtgcgccgct atcaaacgct ccgcttaagc tcgcgcatcg gcttcaagcc
      601 gccatacttt tacaatcgcc cgctacgcaa ccgcctgcac agaatcacct cgccagcgtt
      661 agtcatctgg ggtgaaaaag acggcttcgt gccgcgctct catggcgaga cctatgcgaa
      721 gctaattcca aatagcggtg atttgagaat catcgccgcc gccggtcata gtgcgcatgt
      781 ggagaaggcg gaggaaacgg cgaagatggt gacggagttt cttgcgaact agccagtctg
      841 cgattcacgt tcacgagctt ctaaacgaac cggaaactaa atccatcctg agtaactttt
      901 gagctcagcg gctgccatag gggtccgatg ggcgaagggt tagccgcgct actccagctt
      961 ccgatcctca cgtttggctt cagccatggc ttcgggctca agctcttctg catagcgatt
     1021 caggcgtatg aaaatacccc aggcggcaag cagcacgcca gctgcgacca aaatgctggc
     1081 cgcgtagatc aactgcgccg aatcttcatg tgctgcgcgg aacgtcgcca cgagtccctc
     1141 gatcgcaatt gccacaacga ctactaccag gaacctcgat aggaagcggc gtacacgagt
     1201 ggggccgcta atatgagcat cgcgcacgac ctcttcttca atgatcgtct gcgcgatctg
     1261 gagcgccacg actgcagcag ccagcagtcc tacggcttca atgatctcct gtgcggccgc
     1321 ccggcccaag ctaccggcaa aggcaatcca tgccgctttc gttgcgatgg tgatgagcat
     1381 gagcgcagcg caagcgaaaa gtgccgccat acttgcgtgg ataattgaga aggcgagggt
     1441 gagcagtttc attggcagaa tcgcaacata gacagcgctg aagagtggag ttcgtgcgcc
     1501 ctaaaacaac cgtttttcag tgaaattcat gcacgtgaaa tatcaacata ctcgcacaat
     1561 aaatctttgc gggatctctg tctgtctgct cgactcatca ctcaatgttg gggtcaggac
     1621 atggtttgtg cggcgagcca tctcaggttc cgactgaaga cggcttccac tatcggcgtc
     1681 accgtgccgg caacggatcg gctccggcga ccttctgata gagctggacg acctctttgt
     1741 cgcgcggcaa ttcctttatt gcgcgctcca gttcttcgcc actgagcggc gcagggccgt
     1801 cgcccattaa ttttttaaac tcgacgggaa attccgggtc ggcgaaggac ttattcatcg
     1861 cgtcgcggat gatttgcacg cgatctttgg gcgtgccggg agggaagata taaggccagc
     1921 gcggatattg gaaggcgcgg aacatgtcga gcaatttgcg ctcggtatca gtcttggcga
     1981 agctgccaaa ttccggcaat tgagcgaagc ttgggtgatg gcggcctctg ggaaccgtca
     2041 aagtggcgtg gagatggatc tgcttctttt cgatccactc cggtttttca atgacgaacc
     2101 gggcagcgcc ggtggcatgg gcgtcgattt cgccgcgatc catggcgatg tatatttccg
     2161 gaccggtgaa gccggtgacg aacttgggat ctttcagacc gagcaaatag gcgatgatac
     2221 gccccgtgac gtaaaccgga tggccgaccg agtgggcgcc gaacctgaca ccgctggcgg
     2281 cgcgaagttt ggcaagagaa tccaagcccg catccgcttt agtgtagaac acgtaggggt
     2341 caccgccgtc ggtcgagccc aggtaaacaa acttgtcgag atcgtaggcg ctaccgggca
     2401 aacccaatat cggcccgacc accagtcccg caccaatgga gccgatggtt agtccatcgg
     2461 gtttggtcgt gctgtagatg acattagcgg cttttcttcc cgccgcgccc tcgataaatt
     2521 caaccatgat atgaggtttg ccgggaatat atttttcaag aaacttcacc agcgctctgg
     2581 tctgaaactc acctgagccg ccggggccgc cgccgcggac gaccttgatg gttttacctt
     2641 tgtaaaaatc ggcttgggca aacgcctcgc tggcaagcaa gagcgccgcg aggccaatca
     2701 gccatccatg tacgaaaccg caaaatccta atctactcaa ggacttcatc aatacctccc
     2761 aaaaccctca gttaggcgag tcttcaggtc agtctgttgt tctcttcaat taccataatt
     2821 ccgatagcac tatcatgcaa tatgacgtta cggcaccggc taccggcatc cgcccaataa
     2881 agcttagcta atcgccgccg atactattcc taaggttatc tccggcattg ttatgattaa
     2941 aaaagtcaaa gtcgaccaac tcaagccggg tgtcttcatc gccgatttta actgcggctg
     3001 gttgcaccat ccgtttctca ccgaccgcac caaactcgag accgacaaac agattcagaa
     3061 agtcatcaaa tacggcatcc gcgaactcta catcgacacc gacaaggggt tggatatcga
     3121 tgacgcaccg acacagcggg aagcggacca ggaaattcaa agcgagttta gccggcttaa
     3181 cgcccccgcg gcgggaccta atcgtggcga cggttttaag gcagaactcg cccgggcgca
     3241 ccggctggtc agcgaggcga aactaaccac gaaaaagctc atgcatgaca tcaggctcgg
     3301 taagcgcatg gcgatgaacc aagtggagag cacggtcgat aaaatgaccg actcgatcct
     3361 gcgcaataaa gacgcgttgg tcagcctgct acgcatcaaa gaggtcgatg agtacaccta
     3421 tgtgcactcg atttcggtat ccgctttgtg tatttccttt gcccacggct tgggcctcga
     3481 cgcgacaaaa atcaagcaaa tcggtatcgg cggcttactg cacgacatcg gtaagatgaa
     3541 agtgcccctg gagattctca acaaaccggg accgctttcc gaaaaagaat tcgaagtcat
     3601 gaagcggcat gtcaaagacg gcgaggctgc gctgcaacag tattccaacc tcgatcagga
     3661 gtgcacctgc gtaacctcgc atcatcacga gcgtctggac ggcaccggct acccggatgg
     3721 gctgaaaggc aacgaaatta gtgaatttgg ccagatagcc gcgatcgtcg atatctacga
     3781 cgccctgagc tctgaacggt gctacaaaaa cgccatgccg cccaccgacg cgctaaaaaa
     3841 attgtttgag tggagtcaaa gctacttgaa tcgcgagctg gtcgagaaat ttatcgccca
     3901 tctcggcatc tatccgatcg gcaccctcgt ccgtctggaa aatctcttgc tagccgtcgt
     3961 gatcgaccac ggcgaaaaga acttgcttca tcccgtcgtg cgcgtggttt atgacatgaa
     4021 aaaagcgtcg aggcttgagc cattcgatat tgtccttgaa ggtcaaatca acgcagggaa
     4081 tccctatggc atcgtcggtt gcgagtcccc ggataaatgg cagatcaaac ccgtggacta
     4141 cttgtactaa gcatttctga cgagcccctt cgtcatcagc atctacgaca acctaaccag
     4201 tgccatcagc gatagagcgg gctggtaaaa ttgcttcgtt cagatttatc ggccccagag
     4261 cttttttacg aatccttctt cgtccaaccg tttaagatag cgatcattga cataaagctt
     4321 agcgtcgatg ccacgcgct
//
LOCUS       DASQKS010000420         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_650737, whole genome shotgun sequence.
ACCESSION   DASQKS010000420 DASQKS010000000
VERSION     DASQKS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650737"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1115
                     /locus_tag="VE170_10850"
     CDS             174..1115
                     /locus_tag="VE170_10850"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15978.1"
                     /translation="MKLKLLFLLWLFLFATPYTSEARIIIGLSSVNIAFLPVYVAQEK
                     GFFKDEGLDVQFVMFNSGSTNLQSLMGGDIQMMGSAFVETLGGRAAGYDIKNFWGICN
                     LMPFELYSTAKFTSIKEAKGKRFAISRFGSLTDFLTRATLQHHGIDPKEVTILQIGST
                     PARFAALSAGAVDASVVWFPVTEIAKAQGFRKLFDLKDMFPEWPYETFAARESWLAKE
                     RGDVTKFLRAYQRGVKHTWDSKDDSVRAMRKYVKIDPAYAPAGYDQYRTSFPLNGVIA
                     EKVIPTVIDQEFENGRLKRRITVDEMIDRSFMKALAK"
     gene            1518..2789
                     /locus_tag="VE170_10855"
     CDS             1518..2789
                     /locus_tag="VE170_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYJ15979.1"
                     /translation="MAVAIVAAAVGVGIASTQAIAAESTEDLKLLRQELAKQRAYIEQ
                     LEKRVESQEAKNEARAKAQAEAIAKLPAVEAGYDDGFYIRSKDKPFSLVTNGFAQFRY
                     TFDAPENGHTNQNFDVGLARLALSGMMFDPKLNYFMQLQGSTFGNNNNITMLDWWMKY
                     SFAPEFAVQGGRFVLPYSRQFYTHPGNLLFSDLSEADYAFNLPRGIGAHASGKLGPLS
                     YHVATLNSVRALDAPGQQNIGSNIGALGRLELDILKPYGYYESSPKPVTDPQLSIGVA
                     VAYNPIDGASSFQNLIAKDSTTNVTLDLGFRWDQLTIQGAGYYRRDDFTTPGLSDGND
                     WGYYSQLGYYLVPGKLELAGRISGVEFDKLNVAGVNRKTTAYTGGLNYYIHGHNLKFQ
                     TDYSFLDNDNFRGQPRARDAHRFRLQAQFLF"
     gene            2934..4184
                     /gene="urtA"
                     /locus_tag="VE170_10860"
     CDS             2934..4184
                     /gene="urtA"
                     /locus_tag="VE170_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HYJ15980.1"
                     /translation="MVIAALLAMPLPVRAQSKDTIKIGVLHSLSGTMAISETVLKDVT
                     LMAIEEINAKGGILGRKLEAVVVDPASNWPLFAEKARQLIAQDKVAVVFGCWTSVSRK
                     SVLPVFEELNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYLMSKDGGGAKRFV
                     LLGTDYVYPRTTNKILRAFLKSKNIPEADILEEYTPFSHTDYQTIIGKIKKFAGEGKK
                     TAVISTINGDSNVPFYKELGNQGLKAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNY
                     FMSLKNPTNEEFTKKWAAYAKAKNLPGHKDKPLTNDPMEAAYIGIYMWKQAVEKAKTT
                     DVDPVIAAVGGQTFKAPSGFTIKMDEKNHHLHKPVFVGEIKADGQFNIVWKTPGPIKA
                     KPWSPYIAGNDKKKDEPEIIAKKK"
     gene            4278..>4542
                     /locus_tag="VE170_10865"
     CDS             4278..>4542
                     /locus_tag="VE170_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15981.1"
                     /translation="MITTRWVRVAALSLSVLLFANVSRCIAIDVETAKQLAAEDGDVK
                     LAAIQKLVASADPAAIALLTALQDDVLVLFKGRLIIAGSSGTKD"
BASE COUNT         1114 a         1196 c         1233 g          999 t
ORIGIN      
        1 tcgtttgcgt cgcaccggcg gtgggcaacg cgctgttcaa tgccaccggc gtgcgtgtgc
       61 gcgagctacc gctgactccc gagcgggtct ggcgggcgtt aaacgaatcg gagcggaatt
      121 gacgagtcga ttgatctaac gaatcgtgct ttaacccact aaggagcgat gccatgaagt
      181 tgaagctgtt gtttcttctt tggttgttct tgtttgccac tccgtacacc agcgaggcac
      241 ggatcatcat cggtctttca tcagtgaata tcgcattttt gccggtctac gtggcgcagg
      301 agaaaggctt tttcaaagat gaagggctcg acgttcagtt cgtcatgttc aattccggtt
      361 cgaccaatct acaatcgttg atgggcggcg acatccagat gatgggcagc gccttcgtcg
      421 aaactttagg cgggcgggcg gccggttacg acatcaaaaa cttttggggc atctgtaact
      481 tgatgccgtt cgaactttat tcaactgcaa agttcacttc gataaaggaa gccaagggca
      541 aacgctttgc gatcagccgc tttggctccc tcactgactt cctgacccgc gccaccttgc
      601 agcaccatgg tatcgatccc aaggaggtga cgattttgca aattggcagc actccggcac
      661 gctttgccgc gctctcagcc ggcgccgtcg atgcttctgt ggtctggttt cccgtcactg
      721 agatcgccaa agcccagggc ttccgcaagc tctttgattt gaaagatatg tttcccgaat
      781 ggccctacga gacattcgcc gctcgcgaat cgtggttggc taaggagcgc ggcgacgtga
      841 ccaaattcct gcgcgcttac caacggggcg tcaagcacac ctgggacagc aaggatgact
      901 ccgtgcgggc catgcgcaag tacgtgaaaa tcgatcccgc ctacgcgccg gcggggtacg
      961 atcagtaccg tacttcattt ccgttgaacg gtgtgatcgc cgaaaaagtc atacccacgg
     1021 tcatcgacca agaattcgaa aacggccggc tcaagcggcg catcaccgtc gacgagatga
     1081 tcgaccgatc tttcatgaag gcgttagcga aatgatcgac acgttccccg gcacaacgct
     1141 gaagttttga aagaacttgt tagtcagcca atttaaccgc aataaaaact cgtgcaaatc
     1201 ccccatcagt agtatcgcgc cctacgattt cctgctcgaa atatttcctc tgatcgcaaa
     1261 ataggcatgt gcctacggat tagccggcac atgcaaaata aatgtgcatg aaaccgtgcg
     1321 gcacgcgacg aaaccgcttt gtttcaagta ggttaagttt atgtacgagc aactttcttt
     1381 tcggcatggg agttgctgag cggtcataac gtttgcggaa gacaacgtag tctcctcctc
     1441 gatccaacgg cggatcgaaa cttcatcaag cagtaaatca acgtggggag gagactatga
     1501 gaaaaatatt ggctcgagtg gccgtcgcca ttgtcgcggc ggctgttggt gtggggatag
     1561 cttcgacgca ggcgatcgcg gcggagtcca cggaggatct aaaactcctg cgtcaagagt
     1621 tggcaaagca acgtgcttat atcgaacagc tggagaagcg cgtcgagtcg caggaggcga
     1681 aaaacgaggc gagagctaaa gcccaggcgg aagcaattgc aaaattgccg gccgttgagg
     1741 ccggctacga cgacggattt tatatccgca gcaaggacaa accgttttcg ctggtgacga
     1801 acggctttgc ccagttccgt tacaccttcg acgcgcccga gaatggacac acaaaccaga
     1861 atttcgatgt cggcctggcc cgattagccc tatcaggaat gatgttcgat cctaaattaa
     1921 attacttcat gcagctgcag ggaagcacat tcggtaataa caataacatt acgatgctcg
     1981 attggtggat gaaatattcg ttcgctccag aatttgccgt ccaaggcggt cgctttgttc
     2041 taccgtacag ccggcaattc tacacccatc cgggaaatct gctgttctcc gatttatcgg
     2101 aagctgacta tgcgtttaac ttgccgcgcg gcatcggcgc tcatgcttcc ggcaaactcg
     2161 gcccactttc ctatcacgta gcgacgctta acagtgtccg cgcccttgat gcgcctgggc
     2221 agcaaaatat cggcagtaac atcggcgcgc tcggacgctt ggaactggac atccttaaac
     2281 cttatggcta ttacgagagt tcgcccaagc ccgtgaccga tccgcagctc tcgatcggcg
     2341 tggcggtagc gtacaatccc atcgatggtg cttcgtcatt ccaaaatttg atcgccaagg
     2401 atagcacgac caacgttacg ctggatctcg gtttccgttg ggatcaactt actattcagg
     2461 gtgccggcta ttatcgccgc gacgatttta ctactcctgg attatccgac ggcaatgact
     2521 ggggatacta ctcccagcta ggttactacc tggtgcccgg gaagttggaa ttggccgggc
     2581 ggatctcggg ggttgaattc gacaagctga atgttgctgg ggtgaaccgc aagaccaccg
     2641 cgtacaccgg cggcttaaac tactacatcc atggccacaa tctgaagttt cagaccgact
     2701 acagctttct cgacaacgac aactttcgcg gccaacccag ggcgcgcgat gcgcatcgct
     2761 tcaggttgca agcgcagttt ttattctagc caagatttgt gaccggggaa tttagagaaa
     2821 tgagttatta accgtttgcc agatggagga agttcatggc taccgcattt aagagaatca
     2881 atgtcacggt tcgacaaggc cggcgcattt tttttaagcg cctgggcgcg ttcatggtaa
     2941 ttgcggcatt gctagcgatg ccgctgccgg tcagagcgca gagcaaagat acgatcaaaa
     3001 taggcgtgct tcattcgttg agcggaacga tggcgatcag tgagacagtg ttgaaggacg
     3061 tcaccctgat ggccatcgaa gaaatcaacg ccaagggcgg catattgggt aggaagctcg
     3121 aggccgtggt tgtcgatccg gcgtcgaatt ggccgctgtt tgcggaaaaa gcgcgccagt
     3181 tgattgccca ggacaaagtc gccgtggtgt tcggctgctg gacctcggtg tcgcgcaaga
     3241 gcgttttgcc ggttttcgag gaactgaacg gattgctgtt ctatccggtg caatacgaag
     3301 gcgaggagct ttcgaaaaat gttttctata ccggcgccgc gccaaatcaa caggcgattc
     3361 cggcggtgga gtatttgatg tcgaaggacg gcggcggcgc gaaacgcttc gtattgctcg
     3421 gcacggatta tgtgtacccg cgcaccacta acaaaatcct gcgcgctttc ctgaagtcta
     3481 agaacattcc tgaagccgat atcctggaag aatacacccc gttcagccac accgactatc
     3541 agacaatcat tggcaaaatc aaaaaattcg ccggcgaagg caagaagacc gccgtgatct
     3601 cgaccatcaa cggcgactcc aacgtgcctt tttacaagga gctgggtaat cagggcctca
     3661 aagccaagga tgtcccggta gtggcattct cagtgggcga agaggagctg cgcggcgtcg
     3721 ataccaaacc gctggtggga catctggcgg cgtggaatta tttcatgtcg ctaaaaaatc
     3781 cgactaacga ggaattcaca aagaagtggg cggcctacgc caaagcgaaa aatctcccag
     3841 gccacaagga taaaccgttg accaacgacc cgatggaagc cgcctacatt ggcatataca
     3901 tgtggaagca agcggtggaa aaggcgaaaa ccaccgatgt cgacccggtg attgccgccg
     3961 tgggcggtca aaccttcaag gcgccttcag gatttacgat taagatggat gagaagaatc
     4021 atcatctgca taagccggtg ttcgtcggcg aaatcaaagc cgacggtcag ttcaacatcg
     4081 tctggaagac tccggggcct ataaaggcga agccctggag cccctacatc gccggcaacg
     4141 acaagaaaaa agacgaaccg gaaatcatcg cgaagaagaa gtaacaagca cagccacgcc
     4201 ggaacctata agacgtccgg cgtggctgtt taatccattc gatagagatt tccgctaaca
     4261 accgcctaag catttccatg ataacaacgc gctgggtccg agttgcggca ctctcactct
     4321 ctgtccttct gtttgccaac gtaagccgat gcatcgccat cgacgtcgaa accgccaagc
     4381 aactcgccgc cgaggatggc gatgtcaagc tcgccgccat acaaaaatta gtcgcctcgg
     4441 cggatcccgc ggcgatcgct ttactgacgg cgttgcaaga cgatgttctc gtgctgttta
     4501 aaggacggct aataatcgcc ggcagcagcg gtacaaagga cg
//
LOCUS       DASQKS010000421         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1074060, whole genome shotgun sequence.
ACCESSION   DASQKS010000421 DASQKS010000000
VERSION     DASQKS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074060"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VE170_10870"
     CDS             complement(<1..312)
                     /locus_tag="VE170_10870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ15982.1"
                     /translation="MAANADSQEKKGPLYATFKTSMGDIVIQLFDDKAPKTVANFVGL
                     ASGTKEWTDPKTGEKVKKPIYNGTIFHRVIPGFMIQGGDPLGNGTGGPGFRFEDEFHP
                     DL"
     gene            781..1281
                     /locus_tag="VE170_10875"
     CDS             781..1281
                     /locus_tag="VE170_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ15983.1"
                     /translation="MAQPAQPEPLPTPVSAAPVLQPTAKTEAVPPPQSTAGLIGYLYK
                     GSRVAGQLTFQGSARIDGKVDGEVSCQGTLTIGEEAEVKAKITGQVVIIHGKVEGNVT
                     AKERIELLAPARLIGNIDAPRLVITAGVVLHGDCSMGVAKQKGEVVVARSPSADQAAV
                     AAATKL"
     gene            1444..1872
                     /locus_tag="VE170_10880"
     CDS             1444..1872
                     /locus_tag="VE170_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HYJ15984.1"
                     /translation="MIHLDYSIIYQIVLFVVLWLILDKLLFQPYLRLLDEREHKTTGA
                     QHDSVDLEHEGAKLKADFDEQFAQGQAAGYAAKEAINQEARRQREMILAQARDEAARS
                     LDQVRGEVAAAMEQEKRLAAAEVSTVAADMVHKVLGRRVG"
     gene            1869..2423
                     /locus_tag="VE170_10885"
     CDS             1869..2423
                     /locus_tag="VE170_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15985.1"
                     /translation="MISQDFFGIFMIAQAWAAGGAEHHTPSITEVIFPALNFLIYLAI
                     IVKFALPPIRSYLKNRRDEVVATMTQASAKKAAAQALVAEYKAKLAALDQQVMSLQAT
                     LREEGERDKAKLLGDANTMAAKIKADASVLADQEVKMARQKIREDMAIQAEATARALL
                     QRNLSADDQNRLADEFIQSIGQAR"
     gene            2420..2962
                     /gene="atpH"
                     /locus_tag="VE170_10890"
     CDS             2420..2962
                     /gene="atpH"
                     /locus_tag="VE170_10890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HYJ15986.1"
                     /translation="MIEGSLARRYTRALFQLAREGGQEDAVGVEIEQFFSDYNKSALQ
                     QVLTNPAFAVDTRKQILNQVAQSQKLSSLTVRFLSLLLERDRLGHLTGIVNTYRRLLN
                     EARGRVDAKVVSATALEPAMVERVRGQLRGISGKEVILQQLVDPELLGGLQVELEGKI
                     YDGSVRTQLARMKQRIARGY"
     gene            2973..>4176
                     /gene="atpA"
                     /locus_tag="VE170_10895"
     CDS             2973..>4176
                     /gene="atpA"
                     /locus_tag="VE170_10895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYJ15987.1"
                     /translation="MEIGTAEISRIIKEQIRDYDKTVEIQEVGTVLSTGDGIARIHGL
                     DKVAAGELLEFPHGIFGIALNLEEDNVGAALFGETHMIKEGDTVKRTGRIAEVPVGNE
                     MIGRVVNALGEAIDGRGPIDAKDRRRIELKAPGIVARQPVKEPLQTGLKAIDGMIPIG
                     RGQRELIIGDRQTGKTAVALDAIINQKGGNVICIYVAIGQKRSTVAQVVDKLREAGAM
                     EYTIVVAATASESAPLQFIAPYSGCTMGEHFRDNGGHALVIYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKMSADRGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGGNAQIKAMKQVAGTLRLELAQYREM
                     AAFAQFGSD"
BASE COUNT          953 a         1176 c         1212 g          835 t
ORIGIN      
        1 cagatcggga tggaattcat cctcgaagcg aaaacccggg ccgccggtgc cgttgcccaa
       61 gggatcacct ccttgaatca tgaatccggg gatgacgcgg tggaagatcg tgccgttata
      121 aatcggcttt ttgactttct caccggtctt agggtcggtc cactccttgg tgccgctggc
      181 caggccgacg aaattagcca cggtcttggg cgctttatcg tcgaatagct gaatcacgat
      241 gtcgcccatg ctggttttaa acgtggcgta aagcggccct tttttttcct ggctgtcggc
      301 atttgcagcg atgccgacaa ccgcgcacag cactccaact ataaatagtt ttttcactct
      361 tgcctccgct gattacttct gcgttaattc ggccatcact tcattgacga agcccaggtc
      421 catatatcgc gcacggccgc ttaggtttgg tatggagcgt cacagcttgc catgaaatta
      481 gctctataga atatcgctct aagctaaaca aagtggcgcg agcggtcaag aacatgcccc
      541 actttgttga ttttccggtg accgagagcg gctttttctg gcttttgcga tttaattgga
      601 tcaatgtctg aatatcgagc ggcggccggc agacaagcac tgatcggttc gcgcgccgcg
      661 ttttgacaat aagattctcg gatgttatag gaaactctgc ccaaccactg ggagaaccaa
      721 aaaatggctt ggtttgataa aaaccctggc ggaaaaaaga ccgaaacgga gccgcaaagc
      781 atggcccaac cggcgcaacc ggagccattg ccgactccag taagcgcggc tccagtgctg
      841 cagcctactg ccaagacaga agcagtgccg ccgccgcagt caacggcggg tttgattggt
      901 tatctatata aaggcagccg cgtcgccggt cagctgacgt ttcaggggtc ggcgcgcatc
      961 gacggcaaag tcgacggcga ggtgagttgc caaggcaccc tcaccatcgg cgaggaagcg
     1021 gaagtgaaag ccaaaatcac cgggcaagtc gtgattatcc atggcaaggt cgaaggcaac
     1081 gtcaccgcca aggaacggat tgaactgttg gcgccggccc gcctgatcgg caacatcgac
     1141 gcgccccggt tggtaatcac cgcgggcgtg gtgctgcacg gggactgctc catgggagtg
     1201 gccaagcaaa agggtgaagt cgtggttgcc cgcagcccaa gcgccgacca agccgcagtg
     1261 gctgcagcaa ctaagctgta aactgactgt cagcattcga cttcagttcg ctgcccgtca
     1321 cctcccgatc ctagatccta cgttcaaact agaatgtagg ttttttatta attgggtact
     1381 gggttacgcc atcacgaacg gcggaacccg agcgatagga tgtttgtaag cggtcattga
     1441 tccatgattc atcttgatta cagcataatt tatcagatcg tcctgttcgt cgtgttgtgg
     1501 ctgattctcg ataaactgct atttcagcca tatttgcgtt tgctggacga acgcgagcac
     1561 aaaaccaccg gggcgcagca tgattccgtg gatcttgagc atgaaggcgc caagctcaag
     1621 gccgacttcg atgagcaatt cgcccagggc caagccgccg gttacgcggc caaagaggcg
     1681 atcaatcagg aggcgcgccg tcagcgcgaa atgatcttag cccaagcgcg cgacgaggcc
     1741 gcccgaagtc tcgatcaagt cagaggggaa gtcgctgccg ccatggaaca ggagaagcga
     1801 ctggccgcgg cggaagtcag caccgtggct gccgacatgg tgcataaagt tctcgggagg
     1861 agagtcgggt gatcagccaa gattttttcg gcatattcat gatcgcccag gcttgggctg
     1921 ccggcggcgc tgaacatcat accccgtcga tcactgaggt tatctttccg gccctcaatt
     1981 ttctcattta cctcgccatt atcgtcaagt tcgcgcttcc cccgatacgc agttatctga
     2041 aaaatcgccg tgacgaagtt gtcgccacga tgactcaagc ctcggcaaaa aaagccgcgg
     2101 cccaagcgct ggtggctgag tacaaagcca aattggccgc actcgaccag caagtgatga
     2161 gtctgcaagc tacgctacgc gaggaaggcg agcgggacaa agctaagctc ctcggcgacg
     2221 ccaataccat ggcggcgaag atcaaagccg acgccagcgt tctcgccgat caggaagtaa
     2281 aaatggcgcg gcaaaaaata cgcgaggaca tggcgatcca ggccgaagcc acggcgcggg
     2341 cgttgcttca acgcaacttg tcggcggacg atcaaaaccg cctggcggac gaatttatcc
     2401 aaagtattgg acaggcgcga tgatcgaggg cagcttagca cgcagataca ccagggcgct
     2461 gtttcaattg gcacgcgaag gcggccagga agatgccgtc ggcgtcgaaa tcgaacagtt
     2521 tttctcggac tataataaat cagccctgca acaagtcctc accaatcccg ctttcgccgt
     2581 cgacacgcgc aaacagattc ttaaccaggt cgcccaaagt caaaaacttt cgtctttgac
     2641 cgtaaggttt ctgtcgctgc tcttggagcg cgatcgcttg ggccacttga ccggcatcgt
     2701 caatacctat cggcgcttgc tcaatgaagc gcgcggccgg gtcgatgcta aagtggtcag
     2761 tgcgaccgcc cttgagccgg cgatggtcga gcgcgtgcgt ggccagctgc gcggcatttc
     2821 cggcaaagag gtaatcttgc agcaactggt cgatcccgag ttgctcggcg gcctgcaagt
     2881 ggagctcgaa ggaaagattt acgacggcag cgtgcgaacg caattggcaa ggatgaaaca
     2941 gcgcatcgcg cgcggttatt aaggagtaag cgatggagat cggaacagcg gaaatcagcc
     3001 ggatcatcaa ggagcagatt cgtgactacg ataagacggt tgagatccag gaagtcggca
     3061 ccgtgctttc caccggcgac ggtatcgcgc gcattcacgg cctggacaag gtggctgccg
     3121 gtgagctgct ggagtttccc cacggaattt tcggtattgc gctgaatctg gaagaagaca
     3181 acgtcggcgc cgcattgttc ggcgaaaccc acatgatcaa agagggcgac acggtcaaac
     3241 ggaccgggcg catcgccgaa gtcccggtgg gaaacgaaat gatcggcagg gtggtcaacg
     3301 ccctcggcga ggcgatcgac gggcgcgggc cgattgacgc caaagaccgc cggcgcatcg
     3361 aattgaaagc ccccggcatc gtcgcgcgcc agccggtgaa agagccgctg cagaccggtt
     3421 tgaaagccat cgacggcatg attccgatcg gccgcggcca gcgcgagctg atcatcggcg
     3481 accgtcagac cggcaaaacc gccgtcgctc tcgacgccat catcaatcaa aagggcggca
     3541 acgtcatttg catctatgtt gccatcggtc aaaagcgttc cacggttgcc caggtcgtcg
     3601 acaagctcag agaggcgggc gcgatggaat acaccatcgt cgtcgccgcg accgcatcgg
     3661 aatccgcccc gcttcagttc atcgcgccct attcgggttg caccatgggc gagcatttcc
     3721 gcgacaatgg cggccatgcc ttggttatct acgacgatct gtcgaaacac gcggtagcct
     3781 atcgccaatt gtctctgctg ctgcgccgcc cgccggggcg cgaagcttat cctggcgatg
     3841 tattttacct gcattcccgc ttgctcgagc gcgccgccaa aatgagcgcc gaccgcggcg
     3901 gcggttcact aaccgcattg ccgatcatcg agacccaagc cggcgacgtt tcggcatata
     3961 ttccgaccaa cgtgatttcg atcaccgacg ggcagatctt tttggaaagc gatctgtttt
     4021 actccggtgt ccggccggcg atcaacgtcg gtatctcggt ctcccgggtc ggcggcaacg
     4081 cgcagatcaa ggcgatgaag caggtcgccg gcacactgcg gctggagtta gcgcaatacc
     4141 gtgaaatggc ggcgttcgcc cagttcggat cggact
//
LOCUS       DASQKS010000422         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4420849, whole genome shotgun sequence.
ACCESSION   DASQKS010000422 DASQKS010000000
VERSION     DASQKS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4420849"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VE170_10900"
     CDS             <1..187
                     /locus_tag="VE170_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15988.1"
                     /translation="AEKVEDRAKDQIEYCNEDKWVREKFEGEMSKRGITTLMPPEAYT
                     DPNFEWNWKPFVQESQK"
     gene            complement(309..1220)
                     /locus_tag="VE170_10905"
     CDS             complement(309..1220)
                     /locus_tag="VE170_10905"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15989.1"
                     /translation="MKKIRVATPGYTVSMLPFFAAKMNGYYAAESLDVDLLATRAPTA
                     NLAVLSGSVEFSAVPLAGLTTALRGAPLKLLFVPFDKPQHELFVKPELQGIKALRGKK
                     IAVAGIGTIGEVILREYLTANGLDAGHDVTLLGIGAPETRLASLLTGSFDAAMLIAPY
                     TLNAKEAGFRAMIVFKDHNFVLPSGGIVARDEVLRNDPVTVEKFVRASLMGFLFVRDN
                     RSEAIKVLMRNLKIDESMAANIYDSSRPIMTSDGALSEETQKKMIAFFGKIAGAKDVS
                     SADKVFDFSILRKAQAALQARGWKPAL"
     gene            complement(1472..1816)
                     /locus_tag="VE170_10910"
     CDS             complement(1472..1816)
                     /locus_tag="VE170_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HYJ15990.1"
                     /translation="MALVRCVTEMGMGVDVHGLDYTAAAKRAVFDAIHHSSLGFPRLV
                     GKTADDMRVDVTIGVPKPEAVDRQAVLETLPHGGGHVSVVQGGLQVLNEKGDDGWLIA
                     NAIIVVSIDDGK"
     gene            complement(2047..2952)
                     /locus_tag="VE170_10915"
     CDS             complement(2047..2952)
                     /locus_tag="VE170_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ15991.1"
                     /translation="MKTLRLETTAPFQGLAELVAYDEGLFEKEGIEIEWMDREKGVDK
                     SPQLHITDHKDASRYTSHGRMMESGQADLYNACEWGNFSRVETTNVGSRQVGRRSMVN
                     FAGVVVRADSPVQVPQQFANVQIGVPMYSGTHYLCLQMLEGFVPRELIKIGRVPNGSN
                     YRFKMLMDKTIEATTLTEPYISLAEKMGCRLVISAFHHGTDVASERVDSETYAAFNRA
                     IREAVRRVMSNKRAYMHYFIDYYRDRDPQISQLIVDDLRESRLYLVDPAPIPADELQR
                     TYEWMKSWDFLETAPCAADLVNLNG"
BASE COUNT          651 a          856 c          815 g          648 t
ORIGIN      
        1 cgctgagaaa gtcgaagacc gggctaaaga ccagatcgaa tactgcaacg aagacaagtg
       61 ggtgcgcgaa aagttcgaag gcgaaatgtc caaacgcggg attacgacat taatgccacc
      121 ggaggcttac accgatccga actttgaatg gaactggaaa ccgtttgtcc aagagagcca
      181 aaagtaagct gcgcgctaaa ttccaaaaag ccccactagg catggggctt ttgttggcga
      241 gcagtcaaaa ttccggtggc tgtttccaac tataaactga ggacttgagc cgattgtgat
      301 ggctcgctct ataacgcggg tttccagccg cgtgcctgca aggccgcctg tgcctttcgt
      361 aggatcgaga aatcaaatac cttgtcggca ctggaaacat ccttcgcccc ggcgattttg
      421 ccgaagaagg cgatcatttt tttctgcgtc tcctccgaga gcgccccgtc gctggtcatg
      481 atcggccggg aggaatcgta gatattcgcc gccatcgact cgtcgatttt tagattacgc
      541 atcagaactt tgatcgcttc ggagcggttg tcgcgcacga acagaaaccc catgagcgag
      601 gcgcgaacga atttttctac cgtcaccggg tcattgcgca aaacctcgtc gcgcgcaacg
      661 atcccaccgc tgggcaaaac gaagttgtga tctttgaaga cgatcatggc tcggaatcca
      721 gcttccttgg cgttcaacgt gtatggtgcg atcagcatcg ccgcgtcgaa gctgccggtc
      781 aataggctcg ccagccgagt ttccggagcg ccgatcccca gcagcgtgac atcatggccg
      841 gcatcgagcc cgttggcggt caggtactct cgtaaaatga cttcgccgat ggtgccgatc
      901 ccagcaacgg cgattttctt accgcgcaaa gccttgatcc cttgaagctc gggtttaacg
      961 aacagttcat gttgcggttt gtcgaagggc acgaagagca gtttcagcgg cgcgccgcgc
     1021 agcgcggtgg tcaggccagc gagcggcacc gcggaaaact ccacgctgcc gctcagcacg
     1081 gctagattcg ccgtcggtgc ccgcgtcgcg agcaggtcga catccaaact ctccgccgcg
     1141 tagtaaccgt tcatcttcgc ggcaaaaaaa ggcagcatcg atacggtata acccggagtc
     1201 gctacccgga tctttttcac ctgcgcttgt gcgccgccga tgaaaagcgt cgcgacaaaa
     1261 aataggcaag cggcgaaggt aacttgttta ttgacgaggt gatcgcctgg ggcaaacgtt
     1321 taccaccaag ccttctccaa acctggaaaa ttcatcttgt ggtcgggtct ggaatggcga
     1381 gcgtttgtgg gccgacgtga aataacgtcc tgtaatattt agcgatgcga aaataagcgc
     1441 gatgcggtca tatgacccgc cggggataga attatttacc gtcgtctatg ctgacgacga
     1501 taatggcgtt ggcaatcagc caaccgtcgt cgcccttttc attgagcacc tgcaatccgc
     1561 cctggacgac gcttacgtgg ccgccgccgt ggggcagcgt ttccagcacc gcctggcgat
     1621 ccaccgcctc aggtttgggt acgccgatgg ttacgtccac ccgcatgtcg tcggcagtct
     1681 tgcctaccag ccgcggaaat cccaagctgc tgtgatgaat cgcatcgaac accgcccgct
     1741 tggcggcagc ggtgtagtcc aatccgtgaa cgtccactcc catacccatt tctgtaacgc
     1801 accttacaag ggccatgcta gtcacctcca cctgcgagtt gcagccgact cgattgatac
     1861 cgttactcat tagcaacggg aggactcttc cgtcaagcgt gccgtttcaa ttccagaaat
     1921 tatccgtaac cccgcatgcc attcggcccg gggggttcag aagtttcctt atcgttttga
     1981 actgagcgaa gcagctgaac actggaacga attgaaccaa ctccattccc gctcgtagcc
     2041 ctgctgctac ccgtttaaat tgaccagatc ggcagcgcag ggcgcggtct cgagaaaatc
     2101 ccagctcttc atccactcgt aggtgcgttg cagttcatcg gccgggattg gcgccggatc
     2161 gaccaaatag aggcggctct cgcgcagatc gtcgacaata agctgactga tctgcggatc
     2221 tcgatcacgg tagtagtcga tgaagtagtg catgtaggcg cgcttgttgg acatgacacg
     2281 ccgcacggct tcgcgtatcg cgcggttgaa ggcggcgtaa gtttcgctat caacgcgttc
     2341 ggatgccacg tcggtaccgt ggtgaaacgc cgagatgacg agccgacagc ccattttctc
     2401 cgccaaagaa atatacggtt cggtcagggt cgtcgcttcg atcgtcttat ccatgagcat
     2461 cttgaagcgg taattggaac cgttcggcac gcggccgatc ttgatgagct cgcgcggcac
     2521 gaagccttcc agcatctgca ggcagagata gtgcgtgccg ctatacatcg ggacgccgat
     2581 ctgcacgttg gcaaattgct gaggcacctg cacgggtgaa tctgcgcgga cgacgacacc
     2641 ggcaaaattc accatcgaac gacgccccac ctgccggctt ccgacgttgg tggtttcaac
     2701 gcgcgaaaaa ttgccccact cacaagcgtt gtagaggtcg gcttggcctg attccatcat
     2761 gcggccgtgg cttgtatagc gcgaggcgtc cttatgatcc gtaatgtgca gctgcgggct
     2821 tttgtcgacg cccttctcac ggtccatcca ctcgatctcg atgccttcct tctcgaacaa
     2881 accctcgtca taggccacga gctccgccaa accttggaac ggcgccgtgg tttccaatcg
     2941 cagtgttttc atagattcct ctccgtgggg
//
LOCUS       DASQKS010000423         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2759741, whole genome shotgun sequence.
ACCESSION   DASQKS010000423 DASQKS010000000
VERSION     DASQKS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759741"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VE170_10920"
     CDS             complement(<1..265)
                     /locus_tag="VE170_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYJ15992.1"
                     /translation="MAMERTLSIVKPDAVARNLIGAILKKFESAGLRIAGGKLIRLSP
                     EQAQAFYTVHKARPFFQGLCDYMSSGPIFVSVLEGENAIAKNRE"
     gene            complement(267..1139)
                     /gene="sucD"
                     /locus_tag="VE170_10925"
     CDS             complement(267..1139)
                     /gene="sucD"
                     /locus_tag="VE170_10925"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYJ15993.1"
                     /translation="MAILVNKNTRVLTQGITGATGQFHTKACQEYGTQMVAGVTPGKG
                     GTAYEGIPIFDTVDKAVRETGANATVIYVPPPFAADAILEAADANIPLIICITEGVPV
                     RDMVKVKRFLAGRSSQLLGPNCPGTITPGECKIGIMPGHIHRPGSIGVVSRSGTLTYE
                     AVDQLTRLGLGQSTCVGIGGDPVHGFDFIDVLKLFNDDPATEAVVMIGEIGGSSEEDA
                     AVWITAHMKKPMAGFIAGQTAPSGKRMGHAGAIISGGKGTASEKVTAMKQAGIRMASS
                     PADIGRSMQQALGK"
     gene            complement(1238..2398)
                     /gene="sucC"
                     /locus_tag="VE170_10930"
     CDS             complement(1238..2398)
                     /gene="sucC"
                     /locus_tag="VE170_10930"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HYJ15994.1"
                     /translation="MNIHEFQAKEILKRFGVVVPRGIVASTPEQAKQAALDLGGGVCV
                     VKAQIHAGGRGKGGGVKVVKTPDEAAARAGDMLGKKLVTHQTGAEGRLVRRVLVEQGL
                     NIAREMYLAMVLDRAQSRVSVICSTEGGVEIEEVAEKHPEKILKESIDPVIGLAPFQC
                     RRIASALGIPAELTGKFIAAMQGLYRAFDECDAALAEINPLIITQEGQIMALDAKMNF
                     DSNAFFRQKDILALRDTDEEDPREVEASKFDLSYISLDGNIACMVNGAGLAMATMDIV
                     KVYGGEPANFLDVGGGADKAKVANAFRILLADARVKGVLINIFGGIMRCDVLAQGVVD
                     AARELSIKVPLVVRMQGTNVEQGRKILADSGLPIISAETMAEAAEKIVKAIK"
     gene            complement(2478..3422)
                     /gene="mdh"
                     /locus_tag="VE170_10935"
     CDS             complement(2478..3422)
                     /gene="mdh"
                     /locus_tag="VE170_10935"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020057320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HYJ15995.1"
                     /translation="MARKKIALIGAGNIGGSMAHLALLKGLGDVVLFDVVDGLPQGKA
                     LDLSHAGPIEGFDGAVTGTNKYEDIAGADVCIVTAGIARKPGMSRDDLLGTNAKILSS
                     VAEGIKTHAANSFVIVITNPLDAMVTLMQRKLGFPKNRVVGQSGILDSSRYRSFIAKE
                     LNVSVKSVHAMVLGGHGDDMVPVRSSCIVGGVPVEKLISGERLNEIEARVRNAGGEVV
                     ALLKTGSAYFSPASAAIQMTEAFLFDKKELLPCAALLEGEYGVNGYYFCVPVQIGAGG
                     VEKIIDIGLNAADKKAFNESLSHVKDIVQAMDRVLSAA"
     gene            complement(3443..>3714)
                     /locus_tag="VE170_10940"
     CDS             complement(3443..>3714)
                     /locus_tag="VE170_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYJ15996.1"
                     /translation="YESGHAVFEATHGTAPKYADLDKVNPGSVILSGEMMLRYMGWGE
                     AADLIIKGIEGAVDNNTVTYDFERLMPGAKLVTCSGFGDAIISNM"
BASE COUNT          781 a         1140 c          983 g          810 t
ORIGIN      
        1 cttcgcgatt cttggcgatc gcgttttcgc cttcgagcac cgatacgaag atcggtccgg
       61 atgacatgta gtcgcacaag ccctgaaaaa acggccgcgc tttatgcacg gtataaaaag
      121 cctgcgcttg ctccggcgag aggcgaatca atttgccgcc ggcgattctc agtcccgcgc
      181 tttcgaattt tttcaggatc gcgccgatca ggttacgcgc caccgcgtca ggtttaacga
      241 tcgatagggt tctttccata gccattttac tttcctaatg cctgctgcat cgagcggcct
      301 atatccgccg gcgacgaagc catccgaatt cccgcttgtt tcatcgctgt gactttctcg
      361 cttgctgtac ccttgccgcc ggagatgatc gcgccggcgt gacccatgcg tttgccggag
      421 ggtgccgtct ggccggcgat aaatccggcc atgggctttt tcatgtgcgc cgtaatccaa
      481 accgccgcgt cctcttccga gctgccgccg atctccccga tcatgaccac cgcttcggtt
      541 gccggatcat cgttgaataa tttcaaaaca tcgatgaagt cgaaaccatg caccggatcg
      601 ccgccgatgc cgacacaggt cgattggccc agcccaagcc gggtcagttg atcgaccgcc
      661 tcataggtca acgtgccgct gcgcgagacc acgccgatgc tgccgggacg atgaatatgg
      721 cccggcatga tgccaatctt acattcacca ggggtaatcg tcccaggaca gttgggacca
      781 agcagctgcg agctgcggcc ggcgagaaac cgtttaacct taaccatgtc gcgcaccggc
      841 acaccctcgg tgatgcatat gatcaacggg atgttggcat cggcggcctc cagaatcgca
      901 tccgcggcaa acggcggggg cacgtaaatg accgtggcgt tggcgccggt ttcacgcacc
      961 gctttgtcga cggtatcgaa gatcggaatc ccctcgtaag ctgtgccgcc ctttccgggc
     1021 gtcacaccgg caaccatttg cgtgccatac tcttggcacg ctttagtgtg aaactggccg
     1081 gtagccccgg tgatgccttg ggtcaaaacc cgcgtgttct tgttaactag aatggccatg
     1141 aatttatttt agatttcaga tttagctatt cggacaaatt cactaaagcc ataaggcttt
     1201 cttcacaatt ctcctaagcc aaaatttcga tgcaaaatta tttgattgct ttgacgattt
     1261 tttccgccgc ctccgccatg gtttcagcgg aaataatagg cagcccggaa tcggcgagaa
     1321 ttttccgtcc ctgctcgaca ttggtccctt gcatgcgcac aaccagcggc actttgatgc
     1381 tcaactccct ggcggcatcc accacaccct gggctaacac atcgcaacgc atgataccgc
     1441 cgaaaatatt gatcagcacg cctttgacgc gcgcatcggc aagcaagatg cgaaaggcgt
     1501 tggcgacctt cgccttatcc gcgccgccgc cgacatcgag aaagttagcc ggctcgccgc
     1561 cataaacttt gacgatgtcc atggtcgcca ttgccaggcc ggcgccattc accatgcatg
     1621 cgatgttacc gtcgagggaa atatacgaca gatcgaattt gctcgcctcc acttcgcgcg
     1681 gatcttcttc gtcggtatcg cgcagagcga ggatgtcttt ttggcgaaag aaggcgttgc
     1741 tgtcgaagtt catttttgca tcgagggcca tgatctggcc ctcttgggtg ataatcagcg
     1801 ggttgatctc agccagcgcc gcgtcgcact cgtcaaaggc gcgatagagc ccttgcatgg
     1861 cggcaatgaa tttccccgtg agttcggctg gaataccgag agcggaggcg atccggcggc
     1921 attgaaaggg cgccaaaccg atcaccggat cgatgctctc cttgagaatt ttttccggat
     1981 gtttctcggc gacctcttcg atctcgacgc caccctcggt cgagcaaatg acgcttaccc
     2041 gcgattgcgc gcgatcgagc accatggcga gatacatctc gcgggcgata ttgagcccct
     2101 gctcgaccag cactcggcgc accagacggc cttcagcacc ggtttgatgg gtcaccaatt
     2161 tcttgccgag catgtcaccg gcgcgcgcgg ccgcttcatc cggggttttg acgaccttga
     2221 cgccgccgcc cttgccgcgg ccgccggcat gaatctgcgc tttgaccacg cagacgccgc
     2281 caccgagatc cagggcagcc tgcttcgctt gctccggcgt cgaggcaacg atgccgcgag
     2341 gcacgacgac accgaagcgt ttgaggattt ccttggcttg aaattcgtga atattcattc
     2401 gcttatcgct ggcaaagcga aaccccgaaa ccaacgagtc ggccgcagag tctccctcga
     2461 gtcaccggga agtagggtta cgccgcgctc aaaacgcggt ccatcgcctg gacgatatcc
     2521 ttgacgtgac tgagagactc gttgaaagcc ttcttgtctg cggcgttcaa cccgatatcg
     2581 atgatttttt cgacaccgcc ggcgccgatt tgcaccggca cacagaagta ataaccgttg
     2641 acaccatatt cgccttccaa caaagccgcg caaggcagca gctctttctt gtcgaagaga
     2701 aaggcttcgg tcatttgaat cgccgccgac gccggggaaa aataggccga gccggtcttc
     2761 aatagagcga cgacttcacc gccggcattg cgcacccgcg cttcgatttc attgaggcgc
     2821 tcgccggaaa tcaacttctc gaccggcacg ccgccgacaa tgcacgagct gcgcaccggc
     2881 accatatcgt caccgtggcc gcccaacacc atcgcatgaa cgctcttgac tgacacgttc
     2941 agctctttgg cgatgaacga ccggtagcgc gacgaatcga gtattcccga ctggccgacc
     3001 acgcggttct tggggaagcc gagcttgcgc tgcatcaaag tgaccatcgc atccaacggg
     3061 ttggtgatga cgatgacaaa cgaattggcc gcatgggtct tgatgccctc ggccaccgag
     3121 ctcaaaatct tggcgttggt gccgagcaaa tcgtcccggc tcatgcctgg cttgcgcgcg
     3181 atacccgcgg taacgataca gacatcggcg ccggcaatat cttcatactt attggtgccg
     3241 gtgaccgcgc cgtcgaagcc ttcgatcgga ccggcgtggg aaagatccag ggccttgcct
     3301 tggggcaaac cgtcgacgac gtcgaacaac accacatcgc ccaatccctt gagcagcgcc
     3361 aggtgggcca tcgaaccgcc gatgttgccg gcgccaatta aagcaatctt tttccttgcc
     3421 acaactttat cctctttgaa acttacatgt tggagataat cgcgtcaccg aatccagaac
     3481 aggtaacgag tttcgccccc ggcatgagcc gctcgaaatc gtaagtgacg gtattgttgt
     3541 cgaccgcccc ttcgatgcct ttgatgatca aatcagccgc ctcgccccaa cccatatagc
     3601 ggagcatcat ttccccggag agaatcaccg aacccggatt caccttatcg agatcggcgt
     3661 atttcggcgc cgtaccgtgg gtcgcttcaa acaccgcgtg accgctctcg taat
//
LOCUS       DASQKS010000424         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1076095, whole genome shotgun sequence.
ACCESSION   DASQKS010000424 DASQKS010000000
VERSION     DASQKS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076095"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VE170_10945"
     CDS             complement(<1..620)
                     /locus_tag="VE170_10945"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ15997.1"
                     /translation="MNRETFREQLLGVMERKNHWAWPAFTSGRVPKERLHFHFEQEYG
                     TYVRDFPILVGRSYIRCPIAEIRRSLAENLYEEETGGLIAGRPHPELFLEYPRGLGMD
                     LSRFQNIQLLPAAQRYREFLDVATESFGWDIASAIVTIFVEGSSAERAALDVRQNQPP
                     PPLEDHPLVKYYGLSVDHLALTKAHRQVEGSHRAAAWNAILDHVVPL"
     gene            1006..2553
                     /locus_tag="VE170_10950"
     CDS             1006..2553
                     /locus_tag="VE170_10950"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HYJ15998.1"
                     /translation="MTANYSETSTPLASGTPEHVDNLRVVLNICRRMSAITDLASLQN
                     LITREAKELLQADRVSVFLFDRDNCELWSAISEEGKIMRFDARLGIAGAVAMTGKAIN
                     TANAYEHPLFYKDVDIETGYRTRNLLAVPLYSLDGAVIGVGEATNKIGGDFTDADAEI
                     LTTLAAQLADVIENSPIASELKTHKPEPRSYPAAEFISGFATQKIVGMNHRIQSIIRL
                     IDQIRPSSVDVLIQGESGTGKELIAKALHFNSTRAKYPFVALNCAALPDNLVETELFG
                     IEKGVATGVDRRIGKFEAAHGGTLFLDEIGDLSLSAQAKILRVVQERCIDRVGSRAPV
                     PVDVRIVAATNRDLETAMKEKLFREDLYYRLSVVRIQTPSLREVPEDIPMLANHFLQK
                     HCSASQVEPKQFTSGAVASLSAYTWPGNARQLENEIKRLVASVRSTAIDAGQLLLESR
                     GGHAIAKPAAEPSPGQTLSQAIDALERKMIQQTLDGCDGNKAKSAQILGLSRQGLIKK
                     LRRLQMT"
     gene            complement(2708..2947)
                     /locus_tag="VE170_10955"
     CDS             complement(2708..2947)
                     /locus_tag="VE170_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ15999.1"
                     /translation="MGKGVNLSDAAVRALRKANRKLISANKDLNAAVQALVKASKAKS
                     PAAKAAAARKAYSTVVKRNPLLRAAVKENLKKLAG"
     gene            complement(3040..>4304)
                     /locus_tag="VE170_10960"
     CDS             complement(3040..>4304)
                     /locus_tag="VE170_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16000.1"
                     /translation="ALPAQLYAARFGPKKVKTLILSAPVVRDRDTHVARARMTGANLK
                     LILTTYRAPAGLPCSCGDGKLPVPVQTFLGTAAHKLSEVSAVVNAHSRYNFCFVDPSQ
                     AGVLGDRLEKLLGEIEEQFGSVDFVTDRYDDLLRSADVNSHPRFPREFYAAIKVLQFS
                     GAPETGVMPYSADFARQIDAALVLGHYLTLMEKSASNSAPVARQCAADAPFLNNGRCA
                     AGFCRIIAPAKPTGQRGGQLESMRANQVFGLYDGVARSLLRPGMVERDGNGCFTGKDV
                     TAFASGDGGKQLLRDDIGRLGAVAAEKVCPWTPAKHRHDVRTLILKGGRDSVIAGCQA
                     EDFYRDGLKGERALIEFRGMGHAMYAAPFNLIGDRTVYAAALRVVVERFMDLPVAQLR
                     SDPRVAASLDRLQALFREPDGERTGCPS"
BASE COUNT          942 a         1294 c         1195 g          873 t
ORIGIN      
        1 agcggtacga cgtggtccaa gatcgcgttc caagcggcgg cgcggtggct gccttcaacc
       61 tgtcgatgcg ctttggtcag cgccaaatga tccaccgaca gtccgtaata tttaaccagg
      121 ggatggtctt ccagcggcgg aggcggctga ttttgcctga catcgagcgc ggctctctca
      181 gcgctcgatc cttcgacaaa gatggtgacg attgccgacg cgatatccca gccgaaggac
      241 tcagtcgcga cgtcgagaaa ttctcggtaa cgctgcgccg ccggcaacaa ttggatgttt
      301 tgaaatcggc tcaggtccat gccgagaccg cgcggatatt ctaaaaaaag ctcggggtga
      361 ggccggccgg cgattagtcc gccggtttct tcctcgtaga gattttccgc caagctgcgg
      421 cgaatctcgg cgatgggaca gcgaatatac gatcgcccga caagtatcgg aaagtcgcgc
      481 acgtaggtgc catattcctg ctcgaaatga aaatgcagcc gctctttggg cactcggcct
      541 gatgtaaaag cgggccaggc ccaatgattc ttacgttcca tcacaccgag aagctgttcg
      601 cgaaaggttt cccgattcat cgagacctcc agtagttaaa ggctattgtc tgtccgagta
      661 gtgagcaacc tcagggccag cggataactc cgcgaaataa ctgttttcat gcgccaggta
      721 ccggcatcgg cgcgaagcaa ttagaatatc ccgatgaatc gggccgcatt gccgcaacgc
      781 tgctgatctg acggtggtcc gagataatcc cgggcgaagg acactgcgtt ccgatgtggg
      841 tccctctcca gctcgggcaa cggagcggtc tgcgatcctg cggcaccatt ttgatcaacc
      901 agcgagctat gctgtccctt caatcatatt gttgccagtt tggcatttcg ggttcaatga
      961 gacttttatt ggcagatttt tgccatctgt gctaggcaga aagggatgac tgccaattac
     1021 agcgaaactt ctactccact ggcgagcggc acacctgaac atgtcgataa cttgcgcgtc
     1081 gtgctcaata tttgccggcg catgagtgcg atcaccgacc tggcgtcgtt acagaacttg
     1141 attacccgtg aagccaagga actattgcag gccgatcggg tcagcgtctt tcttttcgac
     1201 cgcgacaact gcgagttgtg gtcggcgatc tccgaagagg gcaagatcat gcgcttcgac
     1261 gcccggctag gtattgccgg cgcggtggcg atgaccggca aagccatcaa cacggcaaac
     1321 gcctacgagc atccgttgtt ttataaagat gtcgatatag aaaccggtta tcgcacccgc
     1381 aatctgctgg ccgtgccgct ttacagcctc gacggcgccg tcatcggtgt cggcgaggcg
     1441 accaacaaaa tcggcggcga ctttaccgat gccgacgccg agatcctgac gacgctggca
     1501 gcccagctcg ccgatgtgat cgaaaactcg cccatcgcca gcgagctcaa gactcataag
     1561 ccggaaccgc ggagctaccc ggccgctgag tttatcagcg gctttgctac ccaaaagatc
     1621 gtcggcatga accatcgcat acaatcgatc atccgcttga tcgaccagat tcgtccgagc
     1681 tcggtggacg tcttgattca aggcgaaagc gggactggta aggaactgat tgccaaagcg
     1741 ctgcacttca acagcacgcg cgccaagtat cccttcgtcg ccctcaactg cgccgccctc
     1801 ccggacaact tggtagaaac cgaacttttt ggcatcgaaa aaggcgtcgc caccggcgtc
     1861 gatcggcgca tcggaaaatt cgaagcggcc catggcggca cgttgttcct cgatgaaatc
     1921 ggcgacctga gcctgtccgc gcaggcgaaa attctccggg tcgtgcaaga acgttgcatc
     1981 gatcgcgtcg gtagccgagc gccggtgccg gtggacgtga ggattgtcgc cgccaccaat
     2041 cgcgatctcg aaaccgccat gaaagaaaaa ctctttcgcg aggatcttta ttaccggctg
     2101 agcgtcgtgc gcattcagac gccgtctctg cgcgaagtcc ccgaggacat tccgatgctg
     2161 gccaatcact tcttgcaaaa gcattgcagc gccagccagg tcgaacccaa gcagttcacg
     2221 tccggggcag tcgcaagctt gagtgcgtac acctggcccg gcaacgcgcg ccaactggaa
     2281 aatgaaataa aacgcttggt cgcatcggtg cgcagcactg ccatcgatgc cgggcagctg
     2341 ttgctcgaga gccgtggcgg ccacgcgatt gccaaaccgg cagccgaacc gtcgccgggg
     2401 caaacgttaa gccaagccat cgatgcactg gaacggaaga tgatccaaca aaccctcgat
     2461 ggctgcgacg gcaacaaagc caaatcggcg cagatactcg ggttaagccg gcagggattg
     2521 ataaaaaagc tgcggcggct acaaatgaca tagcggtgtg attctcactg ccgtcgcgca
     2581 tacctggtta accgatgctt catcggatac cgatgccatg acatataagt cgtcagtccc
     2641 agctcggtta cagtcggcgc tgccgaacga tccgtcgcga tcaggtgaac cgccctgccc
     2701 tcgctctcta gccggcgagc ttctttagat tctctttcac cgcagcccgc agcagcggat
     2761 ttcgtttaac caccgtcgaa taggctttac gcgccgccgc cgctttggca gccggggatt
     2821 tagccttcga tgccttcacc aaagcttgaa ccgcggcatt cagatcttta ttagcgctaa
     2881 ttaacttgcg attagctttg cgcaatgccc tcactgccgc gtccgataag tttacacctt
     2941 tgcccacgtt gcacctcttt ttcacttgac gttagaagtc agacaatagt tcagatcgac
     3001 tgccaaagaa attagcttat tttttcgcta aaccaagttt taactgggac aacccgtccg
     3061 ttcgccatca ggctcgcgaa atagcgcttg caatctgtcg aggctcgccg cgactcgcgg
     3121 gtcgctgcgc agctgcgcca ctggcagatc catgaacctt tccacaacga cccgcaacgc
     3181 cgccgcataa accgtccgat cgccgatcag attgaatggc gcggcataca tggcgtggcc
     3241 catgccgcgg aactcgatca acgcgcgctc acctttgaga ccgtcgcggt aaaaatcttc
     3301 agcctggcag ccggcaatca ccgagtcgcg cccgcccttg agaatcaacg tgcggacgtc
     3361 atggcgatgt ttcgccggcg tccatgggca gactttctcc gccgccacgg cgccgaggcg
     3421 cccgatgtcg tcacgtagga gctgtttgcc gccgtccccg ctagcgaacg ccgtgacatc
     3481 cttgccagta aagcagccat tgccgtcgcg ctcgaccatg cctggccgca gcagcgagcg
     3541 ggcgacaccg tcatacaagc caaacacctg gttggcgcgc atggattcca attggccgcc
     3601 gcgctggcca gtcggctttg ccggcgcgat gatccggcag aacccggcgg cgcatcggcc
     3661 attatttaga aatggcgcat cggcggcgca ctggcgcgcc accggtgcgc tgttcgaagc
     3721 gcttttttcc atgagagtca aatagtggcc aagcaccagc gccgcgtcga tttgcctggc
     3781 gaagtcggct gagtaaggca tgacgccagt ttccggcgcg ccgctaaact gcagcacttt
     3841 gattgccgca taaaactccc gcgggaaacg cggatgggaa ttgacatccg ccgaccgcag
     3901 caagtcgtcg taacgatcgg tgacgaaatc caccgagccg aactgctctt cgatctcgcc
     3961 caagagcttt tccaagcgat cgccgagcac gccggcctgg ctgggatcga caaaacaaaa
     4021 attatagcgg ctgtgggcat tgaccaccgc gctcacttcg ctaagtttgt gcgcggcagt
     4081 gcccaaaaat gtttgcacag gcaccggcaa tttgccatcg ccgcagctgc agggcagccc
     4141 ggccggcgca cggtaagtgg tcaagataag tttcaggttt gccccagtca tacgtgcacg
     4201 ggcaacatgg gtgtcgcgat cgcgcaccac tggcgccgac aagatcagcg tcttcacttt
     4261 tttggggccg aatctagccg cgtacaattg cgccggcagc gcac
//
LOCUS       DASQKS010000425         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2762087, whole genome shotgun sequence.
ACCESSION   DASQKS010000425 DASQKS010000000
VERSION     DASQKS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762087"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..442)
                     /locus_tag="VE170_10965"
     CDS             complement(38..442)
                     /locus_tag="VE170_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16001.1"
                     /translation="MRSRLNSTHCRFSHTVLLLVILASSCAGSRDETIGGITVPVPSA
                     MKKSAEKPLEVSILGFGAGQATFHGAMESAELVEFYKKELPARGWQENLKLLSGGAML
                     AYSKDGKTLLIGANKQDKDTVLSLTVGGIGNK"
     gene            complement(485..1666)
                     /locus_tag="VE170_10970"
     CDS             complement(485..1666)
                     /locus_tag="VE170_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16002.1"
                     /translation="MADSAHSFTREEIAPVITVSLISALRLLGIFLMLPIFSAYAVRY
                     PGATAGLAGVAFGIYALIQCVFQIPLGWASDRWGRKPVLLVGLLLFSLGSIACAWATD
                     IYGLILARALQGTGAVGAVAFAVLADLTRPSVRAQAYTITGIAIGASFMIGLLAGPLL
                     AATIGLNGLFYLLAGLGFLAMILTATSFPPDRRGASAETPITFKAVLAQEKLRPIFTA
                     AFVLSFTLNLFFFTYPLSWTEIGFEKANLWQVYSIIFLPSVLLVFPYMRRAEKAGRFR
                     RPIMIGWFAATVGYAIYLVAANAQYELLLYISGAAFFFGYSIYQPMLPTFLAHQVPAG
                     GRGTASGVYNSFGFIGSSLGGMLGGALTHISPALPEFVGVALLILWFFVGLPVPPEAN
                     S"
     gene            complement(1997..2890)
                     /locus_tag="VE170_10975"
     CDS             complement(1997..2890)
                     /locus_tag="VE170_10975"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYJ16003.1"
                     /translation="MSPIAWESFVMRIMAVGVNAVQGTIRIAVIVATAYVVVKLLRAT
                     LRQFEAFLIRTTSYDEIYPGAAAKRIKTLSSVLWTISSGLLWFVVVLITLSQIGVNIG
                     PILAGAGVVGLAVGFGAQHLVRDLVSGFFLLLENQIRVGDIAIVNGTGGTVEEVTFRT
                     VALRDQSGVVYVFPNGSITTLANATMDWSAYVIDVTVSYNEDTDRIVDIMRRVADDMR
                     KEPEYGRAMIEPIEIYGVDSFTEAAVTIKARFKTQPAQQYAIGREYRRRLKKAFDSEG
                     VELANAPRRAATKGTVPATPA"
     gene            3226..>3997
                     /locus_tag="VE170_10980"
     CDS             3226..>3997
                     /locus_tag="VE170_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16004.1"
                     /translation="MRSKTSGSHGATLLALAFAVLLSIPFTAQGQSAPVKVRLALPTK
                     AVSFLAFYIAHRRGFYKDEGIEFEPILMQPSLASTAVLTGDLDYNGAVTGVIGAAVAG
                     RPMKAVLFTVARPMQYLMSRNDIKDARELKGKKIAGSSPGGTVTFLTKLVLRNVGLDS
                     ERDVFLNPMGGTGASRLAALESGVVDAVILESPDNFLAQRKGFRELLFFGDQVEFPQN
                     GFGTSDKKLKENPDEVFRMVRATLRGLAFSWDKRNHDQV"
BASE COUNT          865 a         1079 c         1225 g          828 t
ORIGIN      
        1 tggcatctag cgatcatcca ccgcctattg ccatcgccta tttatttccg atgccgccga
       61 cggtcaaaga cagaacagtg tccttgtctt gcttgttggc gccgataagc agggttttgc
      121 cgtctttgct ataagcgagc atcgcgccgc cgctaagtag cttcaggttt tcctgccagc
      181 cgcgcgccgg cagttccttt ttgtaaaact cgacaagctc ggcgctctcc atggcgccat
      241 gaaaggtcgc ctggcccgcg ccaaatccta agatcgatac ttccaaaggt ttttccgcgc
      301 tcttcttcat ggcgctcggc acaggaacgg ttatgccgcc gatggtttcg tcccgcgagc
      361 cggcgcagga gctggccaat atgaccagca acaggacggt atgtgaaaaa cggcagtgtg
      421 tggaatttaa tctcgagcgc atcggacctc cagctaactc caactcaatt tggcaaacca
      481 tgatttatga attggcctcc ggcggcaccg gcaggccgac aaaaaaccac aggatcagca
      541 aagcaacgcc gacgaattcc ggcagcgccg gggagatatg agtcaatgcg ccgccgagca
      601 tgccgccgag ggacgagccg atgaagccga acgagttgta aacgccgctc gcggtgcccc
      661 gtccgccggc gggcacctga tgggcgagaa acgttggcag catcggctgg tagatgctgt
      721 agccgaagaa aaatgccgcg ccgctgatat aaagaagcag ttcatattgg gcattggcag
      781 cgaccaaata aatcgcgtag cccaccgtcg ccgcgaacca gccgatcatg atcggccggc
      841 gaaagcgtcc ggctttttcg gcgcggcgca tataggggaa gactaacaag acgctgggca
      901 gaaaaatgat cgaatagacc tgccaaaggt tggctttttc gaaaccgatt tcggtccagc
      961 tcaacggata ggtgaagaaa aataaattca aggtaaagga aagcacgaaa gcggccgtga
     1021 atatcgggcg caatttctct tgggcgagaa ccgctttgaa cgtaatcggc gtttcggcgc
     1081 tggcgccgcg ccgatctggg ggaaatgacg tagcggtcag gatcatggcg agaaatccca
     1141 gccccgccaa caggtagaac aaaccgttga ggccgatcgt agcggcgagc agcggcccgg
     1201 cgagcaggcc gatcatgaac gacgcgccga tggcgatgcc ggtgatggtg taagcttgag
     1261 cgcgcacgct tggacgagtg agatcggcga gcaccgcaaa ggcgacggcg cctaccgcgc
     1321 cggtgccctg gagagcgcgc gctaaaatga gaccgtagat atccgttgcc caggcgcagg
     1381 cgatactgcc gaggctaaaa agcagcaggc cgacgaggag cacgggtttg cgtccccaac
     1441 ggtcgcttgc ccaaccgagc ggaatctgga agacacactg aatcagcgcg tagatgccga
     1501 aggcgacgcc ggcgaggccg gccgttgcgc ccggatagcg caccgcatag gcgctgaaga
     1561 tcggcagcat gaggaaaatc ccgagcaagc gcagcgccga gatcaagctg acggtaataa
     1621 ccggcgcgat ctcttcgcgc gtgaaggagt gagcactatc agccaatgtg aagcgtgatc
     1681 tccgatgccg ttgggataga ccgttaaatt acatgctttg ccggaaatag gaagagggcg
     1741 ttgttggcga tgccgtcaga ttaacgcgct ttgcaccatg gcaattatct ttattcgaac
     1801 tccatactac gatccgtgat agtttgcgcg gcttgcgcag ttactcgata cgttaagagg
     1861 agagcgcggg ttttcaagcc tgccgctata actgccgatg cttttttgcg cctctgcgtt
     1921 ctttggcgat tgttatttgc gcttgcggct tgccgcgcta gactcttcgt gctctcccgg
     1981 tgaatgcctc gaagattcat gccggcgttg ccggaaccgt gcctttggtt gcggcgcggc
     2041 gcggcgcgtt ggctagctcg acgccttcac tgtcgaaggc cttctttagg cggcggcgat
     2101 attcgcggcc gatcgcatac tgctgggcgg gttgggtttt gaaccgcgcc ttgatcgtta
     2161 cggcggcctc ggtgaagctg tcgacgccgt aaatttcgat gggttcgatc atcgcccggc
     2221 cgtattccgg ttcttttcgc atgtcgtccg cgactcgccg catgatatcg acgatgcggt
     2281 cggtatcttc gttgtaggag acggtgacat cgatgacata cgcggaccag tccatggtgg
     2341 cgttggccag ggtcgtgatc gagccgttgg gaaagacata gacgactccc gattgatccc
     2401 gtaaagcgac ggtgcggaag gtaacttctt cgaccgtgcc gccggtgccg ttgacgatcg
     2461 cgatatcgcc gacccgaatc tgattctcga gcaggagaaa aaaaccgctg accagatcgc
     2521 gcaccagatg ctgcgcgccg aagccgacgg cgaggccgac gacacccgcc ccggcaagga
     2581 tcgggccgat gttgacgccg atctggctga gcgtgatcaa caccacgaca aaccacagca
     2641 gtccgctgct gatggtccag agcacactcg ataaagtctt gatgcgcttg gcggcggcgc
     2701 ccggatagat ctcgtcgtaa ctggtggtgc ggatcaggaa tgcttcgaat tgccttaagg
     2761 tagcgcgcag tagtttgacg acgacatagg cggtggccac gatcaccgca atccggatgg
     2821 tcccttgaac ggcgttaacc ccgacggcca tgatcctcat gacgaaagac tcccaggcga
     2881 ttggcgacat gttggtttcc cccttgagcg atagtgctgg aacttttgtc gcaagaaatc
     2941 agtacagata ccaggccgag cggcgaatcg accggtgaaa aatttcttga cttgggttca
     3001 ggctgggata agttgctcga atcctcaagt tggcggaggg atttttctta ggaactaatc
     3061 gagtgacagg aggctagagg agagttgccg gttttccgac gctgcgctgc gcaagagagg
     3121 ttttcatggc gccggaggcg ttcttgcgct cgtcggacac ttgttcagtc agctacttgg
     3181 atgagggtgc ggtggtcaat ctcaacggag gacgataact tggtaatgcg tagcaaaact
     3241 tccggcagtc atggggcaac gctgttggca ctcgctttcg cggttttgct gtcaattcca
     3301 tttactgcac aagggcaatc ggcgcccgtg aaagtccgct tggcgcttcc gaccaaggcg
     3361 gtttccttct tggcgtttta tatcgctcat cgccggggtt tttacaaaga cgagggcatc
     3421 gagttcgagc cgattctcat gcagccgtcg ttagccagca cggcggtgct taccggcgac
     3481 ttggactaca acggcgcggt taccggtgtg atcggtgcgg cggtagccgg ccggccgatg
     3541 aaagcggtgc tgtttacggt cgctcgtccg atgcagtatt tgatgagtag gaatgacatc
     3601 aaggatgccc gcgagctcaa aggcaaaaaa attgccggca gttctcccgg cggcacggtg
     3661 acattcctga ccaaactcgt gttgaggaat gtcggattgg attccgagcg cgatgtcttt
     3721 ctcaatccga tgggtgggac gggagcgagc cgcttagcgg ccctggagtc cggcgtggtc
     3781 gatgcggtga tcttggagag cccggacaac tttctcgccc agcgcaaggg ttttcgcgaa
     3841 ctgctttttt tcggcgacca ggtggagttc ccgcaaaacg gcttcggcac atccgacaag
     3901 aaattgaagg aaaatcccga cgaggttttc cgcatggtgc gcgcgacatt gcgcggcctc
     3961 gcgttcagct gggacaaacg gaatcacgac caggtga
//
LOCUS       DASQKS010000426         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1495063, whole genome shotgun sequence.
ACCESSION   DASQKS010000426 DASQKS010000000
VERSION     DASQKS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495063"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VE170_10985"
     CDS             <1..714
                     /locus_tag="VE170_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16005.1"
                     /translation="AKEVQFDWAKFVWLGSPEQTQRIIYIRGDSPFKTIEDMRGAAEG
                     PRCGATALGTVGHYFPRLVEEALGVKFNLVLGYPGAAEIDLAIQKNEVQCRAGSLEGY
                     FGSEPSKTWAKNGFARVLITGGLKRDPRIADVATLHELMDKRKAPDVVRRLATVLLSP
                     DYIGRPLATTPGVPADRVKLLRDAFNKALTAPELVAEAKTRGWELEPVTGEELETIGK
                     RVMTQPADVIERMKKLLGQ"
     gene            904..2127
                     /locus_tag="VE170_10990"
     CDS             904..2127
                     /locus_tag="VE170_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16006.1"
                     /translation="MYKTTKEENEILTRTGKGTPMGEFLRCYWWPVGISAHLKIKPTF
                     IRVFGEDLVLFRDGNGKAGVIGAYCAHRRANLCLGDVEKDGLRCRYHGWLYDRDGRCR
                     QIPGEPPNSELKKESIRLPAYPVEELGGLIFAYFGPQPAPLVPRYNFLVGDGDVYITI
                     QGMQNCNWLQCVENGMDPVHPSFTHGGAWPDIMSTEPELGFHETEWGMVYKAYRNTKE
                     PGILNYREHHLLMPGVSCGGSGGRYLSGQNANTPVSAARWSVPIDDTHTMLMRVRFKP
                     SDNPGKYEGDPFSRRWKPPQQFIVPYKEYMDSDNPELGYPIPPLHFIEDGMVVDSMPP
                     IADRENENLGPAIDDGIIKLRQMYLREIDKVKRGEDPKCIVRDPEMNKMIVIPAYEKW
                     VAESERNLGIVATVG"
BASE COUNT          491 a          618 c          657 g          422 t
ORIGIN      
        1 gccaaggaag tgcagttcga ttgggccaag tttgtttggc tcggctcgcc ggaacagacc
       61 cagcggatca tttacattcg cggcgattcg ccatttaaaa ccatcgagga catgcgcggc
      121 gccgccgagg gaccgcgctg cggtgccacg gcgttgggca cggtgggaca ttacttcccg
      181 cgcttggtcg aggaggctct cggagtcaaa ttcaatctcg tcctcggcta tcccggcgcg
      241 gcggaaatcg atctggcgat acaaaagaac gaagtgcagt gccgcgccgg cagtttggaa
      301 ggttacttcg gcagcgagcc gagcaaaact tgggcgaaga acggctttgc ccgggtgctg
      361 atcaccggcg gcctcaagcg cgatccgcgc atcgccgatg tcgccacgct ccacgagctg
      421 atggacaagc gcaaagcgcc ggatgtggtc cgccgtctgg cgacggtcct gttgtcaccg
      481 gactacatcg gccggccgct ggcgaccacg ccgggagtgc ctgccgatcg ggtcaagctc
      541 ttgcgcgacg ctttcaacaa ggccctgact gcgccggagt tggtcgccga agcgaaaacc
      601 cgcggttggg aactcgaacc ggtgaccggc gaagaattgg aaaccatcgg caaacgggtg
      661 atgacccagc cggccgatgt catcgagcgg atgaaaaagc tattgggaca gtaggaaaaa
      721 gatgttaacc acaaagggca caaaggtcac aaaaaataat tgctcaatgc gcggcgctcc
      781 gacgtaacct tttgcgtctt ttgcgttcta tgcggccaat tctcagtccg aaccgggccg
      841 gcccccccgc ccctactttg tgatctttgt gccctttgtg gttaaatatc cggagaacag
      901 taaatgtaca aaaccaccaa agaagaaaat gagatcttga cccgcaccgg caaggggacg
      961 ccgatggggg agtttttacg ctgctattgg tggcccgtcg ggatttctgc gcatttgaaa
     1021 atcaagccga cgttcattcg cgtgttcggc gaagatttag tcctgtttcg cgacggtaac
     1081 ggcaaagccg gcgtcatcgg cgcctactgc gcgcaccggc gggcgaattt atgtctcggc
     1141 gatgtcgaga aagacgggct gcgctgccgc tatcacggtt ggttgtacga ccgcgacggc
     1201 cgctgccggc agattcccgg cgagccgccg aacagcgagt tgaaaaaaga atcgatccgc
     1261 ttgcccgcct atcccgtcga agaactgggc ggattgatct tcgcgtactt cggaccgcag
     1321 ccggcgcctc tggtgccgcg ctataatttt ctcgtcggcg acggtgatgt ctatattacc
     1381 atccaaggca tgcagaattg caattggctg cagtgcgtcg agaatggcat ggacccggtg
     1441 catccgagtt tcacccatgg cggcgcgtgg ccggatatca tgagcaccga gccggagctg
     1501 ggctttcacg aaaccgagtg gggcatggtg tacaaggcct atcgcaatac taaagagccg
     1561 ggcatactca actaccgcga gcatcacttg ctcatgcccg gcgtcagctg cggtggcagc
     1621 ggcggccgct atctgtccgg tcagaacgcc aacacgccgg tgtcggcagc gcgttggagc
     1681 gtgcccatcg acgacaccca cacgatgctc atgcgcgtgc gcttcaagcc gtcggacaat
     1741 cccggcaagt atgaaggcga tccgttcagc cggcgctgga agccgccgca gcaatttatc
     1801 gtgccctata aagaatatat ggattccgac aatcccgagc tcggttatcc gatcccgccg
     1861 ctccatttca tcgaggacgg catggtggtc gacagcatgc cgccgatcgc cgaccgcgaa
     1921 aacgaaaatc tcggcccggc gatcgatgac ggcatcatta aattgcgcca gatgtatttg
     1981 cgcgaaatcg acaaagtaaa gcgcggcgaa gatcccaagt gtatcgttcg cgatcctgaa
     2041 atgaacaaaa tgatcgtgat tcccgcctat gaaaaatggg tggcggagag cgagcgcaat
     2101 ttaggaatcg tcgcgacggt gggataggga cgattcacca cgaagacgcg aagagcacga
     2161 agttcggagt atcaattttc gaaacctt
//
LOCUS       DASQKS010000427         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6128547, whole genome shotgun sequence.
ACCESSION   DASQKS010000427 DASQKS010000000
VERSION     DASQKS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6128547"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..471)
                     /locus_tag="VE170_10995"
     CDS             complement(199..471)
                     /locus_tag="VE170_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16007.1"
                     /translation="MQNRNAQIIAGSIVLICHLAGCSHQTVTMIQPGTGATAECSGSS
                     YGFGPLFSEKTADSCARVYQDRGFVPLERLSPEDRASLEQRGLLPK"
     gene            complement(562..696)
                     /locus_tag="VE170_11000"
                     /pseudo
     CDS             complement(562..696)
                     /locus_tag="VE170_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(803..2647)
                     /gene="nhaA"
                     /locus_tag="VE170_11005"
     CDS             complement(803..2647)
                     /gene="nhaA"
                     /locus_tag="VE170_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HYJ16008.1"
                     /translation="MSELNETNYRLNDPVDDKRDHILGPNDAPITLVEYGSYDCPHCR
                     AANERIVQVRGEFGDRLRYVFRHRPISGSDIARRAAELVESIEDPKQFWDAHVALMTR
                     SKTLTEEDLGAVADKLGVARQNAQDAQAAAERAKARVDRDVESARASGVQFTPTFFIN
                     GRRYDGPWDESSFSDAMLAPLGYRVRTAALSFASWAPSTGVLLLLASVLAVGLTNSAL
                     GPSFDAFWHTYFGLMFGKFDFRMPLLDWVNDGVLTIFFLVVGLEIKREFTVGHLASWR
                     SAALPIAAAIGGMVVPALLYKLVIRDGPWSIGWGVPMATDTAFAVALIAMMGRRVPIE
                     LRIFLTAAAIVDDIGAIIVVALFYSGELNFGYLGAAAVITGALALLNRSAVYNVSIYL
                     LLGVGLWACVHAGGLHATLAGVLLAAFIPTRPPPDFRTLAMQADTILAAEARHGGEQL
                     RQGASAPALRALDAIHDRLESPADRLLRNVGPRSSYIVLPIFALANAGVAITPDALHG
                     REPLLLAIMLGLVIGKPLGFMMASAVAVRAGLAQKPNAYSWRHLAGAGALAGIGFTMS
                     LFISGQAFPVPGDFAAAKIAVFTASIMSSLIGIAVLWNAHSHETADGE"
     gene            3137..4219
                     /locus_tag="VE170_11010"
     CDS             3137..4219
                     /locus_tag="VE170_11010"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYJ16009.1"
                     /translation="MESEITNCARRAEQLNGHIRRTGIDPNRWYMIAKASDVLDQPLG
                     REIWRQPIVVFRNRHGELRALEDRCAHRLVRLSHGRVIEDKIECAYHGWQFNDAGRCV
                     HVPHLASKTSLPNCRVRPFPVLERHGFVWIFPGDPAAAASVSPMRMNEWDDLNEIPSW
                     VRLTSRAHFSYLIENLMDMYHGSLHAQYQVWTAQALKEVVEDDDRVTAIYDATTYYRV
                     KDLSSILELFIPPLRKLHSAPLTVTYEYPNWKSTLGDDFKIFCLIRPVNERFTDAYLI
                     HYTSLAKFAGSNKAPPAVRRLIKRVLTNVAKRLLANLVRQDVLMIEEEQQAFDADPHR
                     QPTEINRTVHPVQRLIRRKASAVESR"
     gene            4251..>4695
                     /locus_tag="VE170_11015"
     CDS             4251..>4695
                     /locus_tag="VE170_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ16010.1"
                     /translation="MLKAFLQSRIRNKLGGYGVTINQVDRSKPASVSPAKKVAVVGAG
                     LAGIGAASVLAERGFAVTLFERNSYIGGKVGSWDVAFADGYATKVDHGFHAFFRHYYN
                     LRCFMEKIGAHRRFKTIDDYLVLAHDGKKYSFKNVETTPVLNILSL"
BASE COUNT         1052 a         1420 c         1308 g          915 t
ORIGIN      
        1 aatatagctt tgggactgct tagatttacc cggctgtcaa aatcgaccgg tgatccgacc
       61 gggatgtgag agccgggctg atctcatgac ctaaagcact cgaggtcgtc cgcgagaccg
      121 aaacgtcgaa agccggcgcc aacggtgatt tttgttggct gcgcctgaat aggaacgtgg
      181 tgacggctgc cttcacgcct attttggcag caggccgcgc tgttctaagc tggcgcgatc
      241 ttccgggctc agccgttcga gcggcacgaa accacggtct tggtagacac gcgcgcagct
      301 atcggcagtc ttttcgctga agagaggccc gaagccataa ctggaaccgc tacactcggc
      361 agtagcgccg gtgcctggct ggatcattgt cacagtttga tgactgcatc ctgcgagatg
      421 acaaatcagg acgatacttc cggcaatgat ctgcgcgttg cggttttgca tatattctct
      481 cctgcgactt gaaagcgatt ggcaattcta ccgattccag ctaacccctc aaagagtaaa
      541 tctggattca cttgccggta tattgttcca ctcattccag aagaaatcga tggcgttctt
      601 gagcgcgccg gaagtcgctc ggtcgtactc gggcatcgca aagacgtaag cgtcggcacg
      661 ccgcctgcct ctgctaaggg gacgcgggtt cgtcaagtag taaaaaactc aagtctgtga
      721 cggagagcga tccaaaacgg cgcgcactca gccgcaccaa taaacataat gcgctcttct
      781 gcctgatcgc gctcggtgac gatcattctc cgtctgcggt ttcgtgactg tgggcattcc
      841 atagcaccgc aatgccgatc aggctcgaca ttatcgatgc ggtgaacaca gcaatcttgg
      901 cggcggcgaa atcgccgggt accgggaacg cctggcccga aatgaaaagc gacatggtaa
      961 agccgatacc ggccagcgcg ccggcgccgg cgagatgccg ccaggaatat gcgttgggct
     1021 tttgcgcaag cccggcgcgc acggccacgg cggacgccat cataaatccc aacggcttgc
     1081 cgatcaccag tcctaacatg atcgccagca gcagcggctc tcggccgtga agcgcatccg
     1141 gcgttatcgc cacacccgcg ttcgccaacg caaagatcgg cagcacgata tagctcgaac
     1201 gcggaccgac attgcgcagc agccggtccg ccggtgattc cagacgatca tggatcgcgt
     1261 ccaaggcgcg cagcgccggc gccgaagccc cttggcgcaa ttgctcgccg ccgtggcgcg
     1321 cttcagctgc caggatcgta tccgcctgca tcgccagagt tctaaaatcc ggcggcggcc
     1381 gggtcggaat aaacgcggcc aggaggacgc cggccaatgt cgcgtgcaaa cccccggcgt
     1441 ggacacaagc ccacagaccg acgcccagca ataaataaat tgacacatta taaacggccg
     1501 atcggttgag caacgccaac gctccggtga tcactgccgc agcgccgagg taaccaaaat
     1561 tcaactcacc ggaataaaag agagccacaa cgatgatagc gccgatgtcg tcgacgatcg
     1621 cggccgcggt caagaagata cgcagctcga tgggcactcg tcttcccatc attgcgatca
     1681 gagccaccgc gaaggcggtg tctgttgcca tcggcacgcc ccagccgatg gaccacggcc
     1741 catctcggat gaccagctta taaagaagcg ccggcaccac catgccgccg atcgctgctg
     1801 caatcggcaa tgcagcggag cgccagctag ccaaatgacc gacggtgaat tcgcgtttga
     1861 tctcaaggcc gacaacgagg aaaaagatcg tcagcacacc gtcattgacc cagtccagca
     1921 acggcatccg aaaatcgaat ttgccaaaca taagcccgaa ataagtgtgc caaaatgcat
     1981 caaagcttgg accaagcgcc gagttggtga ggccaaccgc cagcaccgac gcgagcagca
     2041 gcaacacacc ggtggacggc gcccaactgg cgaagctcag cgcggcggtg cgcacgcgat
     2101 agccaagcgg cgcgagcatg gcatcggaaa aagagctttc atcccagggg ccatcgtagc
     2161 gccgtccgtt gatgaagaag gttggggtaa attgcacacc gctggcgcgg gcgctctcga
     2221 catcccgatc aacacgcgcc ttagcgcgtt cggctgccgc ttgcgcatct tgcgcatttt
     2281 gccgcgccac gcccagctta tctgcgacgg cgccaagatc ttcctcggtg agggttttcg
     2341 agcgcgtcat caacgcgaca tgggcgtccc agaactgctt aggatcctcg atggattcga
     2401 ccagttcggc ggcgcgccgc gcaatatcgc tgccgctgat cggccgatgc cgaaacacat
     2461 agcgcaatcg gtcgccgaac tcgccgcgca cctgcacgat gcgctcgttc gcggcgcggc
     2521 aatgcgggca gtcgtaactg ccatattcca ccagcgtgat cggagcgtcg ttggggccga
     2581 gaatgtgatc ccgcttgtcg tccaccggat cattgagacg atagttggtt tcgtttaact
     2641 ctgacatctc agctccaatg aagacgccga ggcatggcgt gcattctctt ttattcttgt
     2701 cgggtcattc aacaacgaag gaattccttc gcctggccgt cggcgcgcgc gtctgaagaa
     2761 tcaggtagat cagattgcac taacatatcg acaaaactac gcagcatatg cggcgaattc
     2821 aaactcatga gataatcgct gcgccaatgt ttaaggctga aacaggagcg agagtcattt
     2881 acgatcgctt gggcgcccgg gtgattcgcg aggaaatgca tagatccaaa tttttccata
     2941 gcagggcaat cccggcgagc aactcacaaa aatcttgccg tctggcagca agactcgccc
     3001 tcggaattgg cgaggattca gtggagtcgc ggcgtgcggt tatgaccctc tcgggtgcct
     3061 ccggactcgc tgccttgtgc cgccaaatgt tttatcttat ctttcatgct tcgatgcgcg
     3121 gacccttgac aaccccatgg agtcagagat cacaaattgc gccaggcgcg ctgagcagtt
     3181 gaacggccac atccgccgca ccggcatcga tcccaaccgc tggtacatga tcgccaaggc
     3241 atccgacgtc ctcgaccagc cgctcgggcg ggagatttgg cgccaaccca ttgtcgtctt
     3301 tagaaaccgc cacggcgagc tgcgcgcgtt ggaggatcgc tgtgcgcacc ggcttgtgcg
     3361 gctcagccac ggccgcgtca tcgaagacaa gatcgaatgc gcttatcacg gctggcaatt
     3421 caacgacgcg ggccgatgcg ttcacgtgcc gcatctcgcg tccaaaacca gcctgcccaa
     3481 ctgtcgggtt agaccgttcc ccgttctcga aaggcacggc ttcgtgtgga tttttcccgg
     3541 cgatcccgct gccgccgcgt ccgtgtcgcc gatgcgtatg aacgaatggg acgatctcaa
     3601 cgaaattccc agttgggtaa gactcacttc gcgcgcgcat ttttcttacc tgatcgaaaa
     3661 cttgatggac atgtatcacg gcagcctgca cgcccagtat caggtgtgga ccgcgcaagc
     3721 gctcaaggag gtcgtcgaag acgacgatcg agtgacggca atttatgacg ccaccactta
     3781 ctatcgcgtg aaggatctca gttcgatttt agagctgttt attccgccgc tgcgcaagct
     3841 tcactcggcg cctttaaccg tgacctatga atatcctaac tggaaatcga cgttgggcga
     3901 cgatttcaaa atcttttgtc tgatccgacc ggtgaacgag cgcttcaccg acgcctatct
     3961 tattcactac acttcccttg ccaaatttgc cggctcgaat aaggcgccgc ctgccgtgcg
     4021 gcgtctgata aagcgggttc taaccaacgt ggcaaaacgg ctgctggcca atctagtgcg
     4081 ccaggatgtc ctgatgatcg aggaggagca gcaggcattc gacgccgacc cgcaccggca
     4141 gccgactgaa atcaaccgca ccgtccaccc cgttcaacga ctcatacgcc gcaaagccag
     4201 cgccgtggaa tcccgatagt tgggctcgcg catcccggga attgacaacc gtgctcaaag
     4261 ccttcctaca atcgcgcatc agaaacaaac ttggcggcta tggcgtaacc atcaaccaag
     4321 tcgaccggag caagccggca tcggtttcgc cggcgaagaa agttgccgtg gtcggggccg
     4381 gcctcgccgg catcggcgcc gcctctgtgc tcgcggaacg cggcttcgcg gttacgcttt
     4441 tcgagcgaaa ttcgtacatc ggcggtaagg tcggctcgtg ggatgtcgct ttcgccgacg
     4501 gctatgcgac caaagtcgac cacggctttc acgccttttt tcggcactac tacaacctgc
     4561 gttgcttcat ggaaaaaatc ggcgcgcacc gacgcttcaa gaccatcgac gactacctgg
     4621 tcctggcgca tgacggcaaa aaatatagtt tcaagaatgt cgaaaccacg ccggtgctca
     4681 acatactgtc actgg
//
LOCUS       DASQKS010000428         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3173835, whole genome shotgun sequence.
ACCESSION   DASQKS010000428 DASQKS010000000
VERSION     DASQKS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173835"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VE170_11020"
     CDS             complement(<1..368)
                     /locus_tag="VE170_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16011.1"
                     /translation="MPPVSRGYRLNGLTARLASYKRMIYGKWQLAAASNGIVGGFLRR
                     LGLSIVFICWLGAGAQAQSNFYEDKTIRLVIGFSAGGISDLWGRALARAMSQYIPGKP
                     QIVLHNMPGAGSLTAANYLY"
     gene            503..1582
                     /locus_tag="VE170_11025"
     CDS             503..1582
                     /locus_tag="VE170_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYJ16012.1"
                     /translation="MTIALSHGGSTIYSSPTLSNHILVGTREGAVSIERDAPGAPWRI
                     GPRSLTDKHVSAIVKEPESGLAFAGAFHGGVMVSADDGKSWEARDHGLSQNNVYSLAA
                     KNMNGHVRLFAGTEPAHLFVSEDLGLNWRELPALRSVPSVPKWSFPAPPHVGHVKHIN
                     FDPDNPNTIYASVEVGGLLRSTDGGESWDEFPGLYEDVHRLMVHPADPSFLYAVTGRG
                     LYTAPERGARWEQWTKREDEIGGYPDGFVFRPSDPKLLFMTAAHDAPGTWRNTHFAGA
                     RISRSKDGGRSWEILKNGLPDRLQASIEAFCLEEAGSSTEIYAGTTAGEILCSQDLGD
                     SWKVAVKGLAPISKGGHYRALVQAA"
     gene            complement(1592..2455)
                     /locus_tag="VE170_11030"
     CDS             complement(1592..2455)
                     /locus_tag="VE170_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16013.1"
                     /translation="MLTNEPIAYLRRPPVLLVTGGDSGIGLEIARKFAESGYRVAIGG
                     LDRRKGDRVVTTLRALGGDAAYFLADVRSEYQIRTLIRRAVQRFGRIDVLCNNAGIRR
                     LAPVDKASAAQWNDVMAVNARAVFFSSKYGLPHLKWSRGTIINIASTAGLVGYAGGLA
                     YCASKAALVMMSKTAALEFATYGIRVNCICPGATRTPLIPAPQIKKLSSRFPSGGSAN
                     RATSPSWRYFLPRKKPGRSPAVSTSSTAAPRPAGRSWREKFRTPEDWPQRTQRARTRI
                     VGAKNFSPRVS"
     gene            complement(2491..3207)
                     /locus_tag="VE170_11035"
     CDS             complement(2491..3207)
                     /locus_tag="VE170_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ16014.1"
                     /translation="MITKEDVLQAVAESWWEAKPKIIEFVNHHLTREGARIFTPEHTY
                     FAAHFPRWFGNVIANCPDIGPRSYMIGNIYVEEVDDPTIHDTHHGSMVTFGEGLGMTR
                     EQVLNHVPSIPMRMGIHYWDNMSRTRPWLEGFAAVGSLELTNHGALAARYGEIALNAR
                     KKWQPLGLDSKYLVHWEAADAADPGEGGHADETVRILVQYAKNDDEGRRVLEVVRESI
                     SVFRFIYDHIAERAIAAGKQ"
     gene            complement(3329..>4380)
                     /locus_tag="VE170_11040"
     CDS             complement(3329..>4380)
                     /locus_tag="VE170_11040"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16015.1"
                     /translation="HWHEKDGGRYIGTFDAVISRDPDSGYVNVGTYRVQVHDDKTVGL
                     WIIPGKHGNLIAQKYWSKGQDCPFLIACGVPPSMLLASAVGIPWGVSEYDFLGGLLGA
                     PVPIAIGKQTNLPMPANAEVVLEGFAPPPDKVSHPEGPFGEWPGYYASGTVDRPVVKV
                     STVYHRNDPIVTGDPPLKTYLNTDIYMYIRSANIWSAMERGGIPDIHGVWFPRQGRFV
                     VAVAIRQRYPGHARQAAHGVLATRDGGRDTRMIIVVDDDIDITNINELLWAVASRWDP
                     KRQSEIVEVPASDLNPTVTPEQRAAGEMVSSCQIIDACRPMARKDDFPQVSSVSPEYR
                     AKVLQQWGHLFATKA"
BASE COUNT          871 a         1393 c         1265 g          851 t
ORIGIN      
        1 ctatacaggt aattcgccgc ggtcagggac ccggcgccgg gcatgttgtg aagcacgatc
       61 tgcggcttgc ccgggatata ctggctcatc gcccgcgcca acgcccgtcc ccaaagatcg
      121 ctgatgccgc cggcggagaa accgatgacc agccgaatgg tcttgtcttc gtagaagttg
      181 ctctgcgcct gggcccctgc gccaagccaa caaataaaca ctatcgatag cccaaggcgt
      241 cgcaaaaatc ctccgacaat cccgttcgat gcagcggcca attgccattt gccgtagatc
      301 attcgtttat agctcgctaa cctcgccgtc aagccgttca gccgataacc gcgcgatacc
      361 ggcggcaagt ttcatccagc cattaaaaca acggttatgc caacggccaa accgccttga
      421 cagaaaaaca gcgcggtgga atggtgggcg cagtctgcgg catcattgcg ccgcaagaac
      481 cgatcatcag gagggcaaaa ccatgaccat cgccttgtcc cacggcggct ccaccatcta
      541 ttcatcaccg acactttcca atcatatcct cgtcggcact cgcgaaggcg cggtcagcat
      601 cgaacgtgac gcgccgggtg cgccatggcg catcggcccg cgctcgctca ccgacaaaca
      661 tgtcagcgcg atcgtgaaag aaccggagag cggcctggcc ttcgccgggg cgtttcacgg
      721 cggcgtcatg gtcagcgccg acgacggcaa aagctgggaa gcgcgcgacc acggcctgtc
      781 gcaaaacaat gtctactcgc ttgctgccaa aaatatgaac ggccatgtgc gcctgttcgc
      841 cggcaccgag ccggcgcatc ttttcgtcag cgaagacttg ggtctcaact ggcgggagct
      901 gcctgccctc cgttccgtgc cgagcgtgcc caagtggagc ttcccggcgc caccccatgt
      961 cggccacgtc aaacatatca atttcgatcc cgacaacccg aatacgatct atgcgtcggt
     1021 cgaagtcggc ggtttgttgc gcagcaccga tggcggcgag agttgggacg aatttcccgg
     1081 cctctacgaa gacgtgcacc gcttgatggt gcatccggcc gacccgagct ttttatatgc
     1141 ggtcaccggc cgcggccttt acaccgcgcc cgaacgcggc gcgcgctggg aacagtggac
     1201 caagagagaa gacgaaatcg gcggctaccc cgacggcttt gtctttcgcc cgagcgaccc
     1261 gaaactgctt ttcatgaccg ccgcccacga tgcgccgggc acctggcgta acacccactt
     1321 cgccggcgcg cggatctcgc gcagcaagga cggcggccgc agttgggaaa tactgaagaa
     1381 cggtctcccc gaccgcctgc aagccagcat cgaagcgttc tgtctagaag aagcgggatc
     1441 ttccaccgag atctacgccg gcaccaccgc cggcgaaatc ctctgcagcc aggacctcgg
     1501 cgacagctgg aaagtcgcag tcaaaggact ggcgccgatc tccaagggcg gacattaccg
     1561 cgcgctggtg caagcggcat aatttacggc ttcaactaac tcggggcgaa aaatttttcg
     1621 ctccgacaat cctagttcgt gccctttgcg ttctttgcgg ccagtcttcc ggagtcctaa
     1681 atttctcacg ccagctgcgg ccggccggcc gtggtgccgc cgtcgatgac gtagacaccg
     1741 ccggtgatct gccgggcttt ttccgaggca agaaataacg ccaactcggc gacgtcgccc
     1801 ggttcgccga tccgcccgag gggaatctgc tggacagttt tttgatctgg ggcgccggta
     1861 tcaacggcgt gcgcgtcgcg ccggggcaga tgcagttgac ccgaatgcca taggtggcaa
     1921 attccagggc ggcagttttg ctcatcatca ccagagcggc tttcgacgca cagtaggcca
     1981 aaccgccggc gtagcccacc aggcctgcgg ttgaagcgat attgataatc gttcctctgc
     2041 tccacttgag gtggggcaag ccgtatttgc tgctgaaaaa taccgcgcgc gcattgaccg
     2101 ccatgacatc gttccattgc gccgccgaag ccttgtcgac cggcgccagc ctgcgaatgc
     2161 cggcgttgtt gcacagaaca tcgatgcggc caaagcgttg cacggcgcgg cgaatcagtg
     2221 tgcgaatttg gtactcgctg cgcacgtcgg cgagaaaata ggccgcatcg ccgcccagcg
     2281 cccgcagcgt cgtcaccacc cgatcgccct tgcgccgatc caagccgccg atcgccaccc
     2341 ggtagcccga ctcggcaaac ttgcgcgcaa tctcaaggcc gatgcccgaa tcgccgccgg
     2401 tcacgaggag cacaggcggt ctgcgcaggt atgctatcgg ttcattggtt aacattggcg
     2461 ttgccaagcg gtctccaagc cgcggcggcg ctattgcttg cccgccgcga tcgcccgctc
     2521 ggcgatgtgg tcataaatga aacgaaacac gctgatgctt tcgcgcacta cttcgagcac
     2581 gcgccggcct tcatcgtcgt tcttggcata ctgtacgaga atgcgcacgg tttcgtcggc
     2641 atggccgccc tctcccggat cggcggcatc cgccgcctcc caatggacga gatatttgct
     2701 gtcgagcccc agtggctgcc atttttttcg agcattgagc gcaatttcgc cgtagcgcgc
     2761 ggccagcgcg ccgtgattgg taagctccag cgagcccacc gcggcaaagc cttcgagcca
     2821 aggccgggtg cgactcatgt tgtcccagta gtgaatgccc atgcgcatcg gaatgctcgg
     2881 aacgtgattg agcacctgct cgcgcgtcat gcccaaacct tcgccgaaag tcaccatgct
     2941 gccatgatgg gtgtcgtgaa tcgtcggatc gtcgacttcc tcgacgtaaa tgttgccgat
     3001 catgtaggag cgggggccga tatccgggca gttggcgatg acattaccga accagcgcgg
     3061 gaaatgcgcc gcgaaatagg tatgctcggg cgtaaagatc cgcgcccctt ctctggtcaa
     3121 atgatggttg acgaactcga tgatcttcgg cttggcttcc caccatgact cggcgaccgc
     3181 ttgcaagacg tcttctttgg taatcatctt cgcttccttc tatctatctg caactggcga
     3241 taaaccccgg aaagatttct cgttgtgctc gaaatgacag gggaagcata ctttctgtta
     3301 cctcgaacca aacgcgagat tggcccggtc acgctttcgt ggcaaataaa tgaccccact
     3361 gctgtaatac tttcgcgcgg tactccgggc tgacactgct cacctgggga aagtcgtcct
     3421 tgcgcgccat cggccggcaa gcgtcgatga tttggcacga gctcaccatc tctccggccg
     3481 cccgctgttc cggcgtcacg gtcggattca aatcgctggc cggcacttcg acgatctccg
     3541 actgccgttt cgggtcccag cgcgacgcca ccgcccacaa cagctcgttg atattggtga
     3601 tgtcgatatc gtcgtcgacg acgatgatca tgcgcgtgtc gcgtccgcca tccctggtgg
     3661 cgagcacgcc gtgggctgcc tggcgcgcgt ggccggggta acgctggcgg atcgccaccg
     3721 cgacgacgaa gcggccctgg cgtggaaacc agacgccgtg gatgtcggga atgccgccgc
     3781 gctccatcgc cgaccagatg ttcgccgatc ggatgtacat gtagatgtcg gtgttgagat
     3841 aagtcttgag cggtggatcg ccggtaacaa tcggatcgtt gcgatgatag accgtcgaaa
     3901 ctttcaccac cgggcgatcg accgtgcccg acgcgtagta tcccggccac tcgccgaacg
     3961 ggccctcggg gtgggaaact ttatccggcg gcggcgcgaa accctcgaga actacttcgg
     4021 cattggccgg catcggcaag ttggtctgct tgccgatcgc gatgggcacc ggcgcaccca
     4081 aaagtccgcc gagaaaatca tactcgctca caccccaggg aatgcccacg gcgctcgcca
     4141 acagcatcga cggcggcacg ccgcaggcga tcaggaaggg acagtcctgc cccttgctcc
     4201 aatatttttg cgcgatcaaa ttgccgtgct tgccgggaat gatccacaag ccgacggttt
     4261 tatcgtcatg cacttgcacc cggtaggtgc cgacattgac atatccgcta tcgggatcgc
     4321 ggctgatcac ggcgtcaaaa gtaccgatat agcggccgcc gtctttttca tgccagtgcg
//
LOCUS       DASQKS010000429         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4427120, whole genome shotgun sequence.
ACCESSION   DASQKS010000429 DASQKS010000000
VERSION     DASQKS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4427120"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1046
                     /gene="fmt"
                     /locus_tag="VE170_11045"
     CDS             105..1046
                     /gene="fmt"
                     /locus_tag="VE170_11045"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYJ16016.1"
                     /translation="MAAPWRIIFMGTPQIAAATLAHILTGPDTVVGVVTQPDSSAGRG
                     QKISPSPVRKLAASYAIPVIAAEKIRTPEFIQSLRAWQPDIIIVVAFGRILPKTVLEL
                     PPQGCVNVHYSLLPKYRGAAPAAWTIINGEAEAGVSTMRLVEKMDAGSIYLQEAIRLS
                     GEETTGSLQTQLTPIGARLLLETLRRLKAGTLGVTEQDESRVTLAPILRKEDGLIDWQ
                     RPAIEIERRIRGFDPWPGSFTHFAGKLLKIHRATIVGTERRGGPGEIIRADAAGFWLA
                     TSSGVLSLEEVQQENKKRLAGIEFIKGARIKPGDRLE"
     gene            1039..2391
                     /gene="rsmB"
                     /locus_tag="VE170_11050"
     CDS             1039..2391
                     /gene="rsmB"
                     /locus_tag="VE170_11050"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HYJ16017.1"
                     /translation="MNDGKNVRQIAGEILHRVESQKSYADLLLDHHLRSDDFNDADRS
                     LLTELTYGTLRWRGKIDAQLLPFLNRSLAETDSFNRNLLRVTLYQLLFLDRIPAYAAV
                     NEAVEIAKATQPGSASFVNGVLRSFLRHLAKGGMPTDTQSSVANLADEYSHPQWLVNR
                     WREYFGDEQAKALMLASNQKTPLVVRTNLGKTSRKELLACWHEQDIAAEASAVSPQAI
                     RLPSGLSIEMLPGFNNGLFQVQSEASQLVSYLVGPASGDLVLDACAAPGGKATHLAEL
                     IGDQGNIVAIDTSARGVERIEQNAQRLGLATIQAECANASKALPNHLAGPYDCILVDA
                     PCSGLGTLRSHPEIKWQRDDSDIARLAALQARIVQSVAQYLKVGGVLVYSTCTLTVDE
                     NERVVDNFLRENSRFELVDAARYLPEAARHMVHGKYFQALPQRDDTDGFFAARMRKVT
                     "
     gene            2392..3051
                     /gene="rpe"
                     /locus_tag="VE170_11055"
     CDS             2392..3051
                     /gene="rpe"
                     /locus_tag="VE170_11055"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYJ16018.1"
                     /translation="MPGIKIAPSILSADFSRLKDEIQAVESAGADWIHVDVMDGHFVP
                     NITIGPVVVEWVRKVTTIAVDVHLMITDPDKYAPEFIKAGADWLSIHPDTCTDPNTTL
                     RKIRNLGAKASIAVNPDVPLSKVVGCFDNVDMILMMTVFPGFGGQAFIADVLKKITEV
                     RKVIDAGRPGVLIEVDGGIKTDNIAAVVQAGGDVIVSGSGIFKTPSYAETIRQMRQAV
                     A"
     gene            3203..3279
                     /locus_tag="VE170_11060"
     tRNA            3203..3279
                     /locus_tag="VE170_11060"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3237..3239,aa:Pro,seq:cgg)
BASE COUNT         1000 a         1181 c         1156 g         1010 t
ORIGIN      
        1 tgcgcagtgg cattgagccg gctcacgaaa cagcgcacat catgatctaa gcgcggccat
       61 cagtttcttg aataactgcc gcatcgcttc gtcacgtcat tttcatggca gcaccttggc
      121 gcatcatctt tatgggcacg ccgcaaatcg ccgcggcaac tttggcgcac atcctcaccg
      181 gcccagatac cgttgtcgga gtggtcaccc agccggatag cagcgccggg cgcggccaga
      241 aaatttctcc ctcgccggta cgaaaattgg ccgcgtcata tgccatcccg gttattgccg
      301 cggaaaagat tcgcacaccg gaatttattc aatcactgcg cgcatggcag cctgacatca
      361 tcatcgtagt cgcctttggc cgcattctac cgaaaacagt tttagaactc ccgcctcaag
      421 gctgcgtcaa cgtccattat tcgctcttgc cgaaataccg cggtgcagcg ccggccgcgt
      481 ggacgattat caacggcgaa gctgaagccg gtgtcagcac catgaggctg gtggaaaaaa
      541 tggatgctgg gtcgatctat ttgcaagagg ctatcaggct cagcggtgag gaaactaccg
      601 gatcactgca aacccaactg acacccatcg gggccagact attgctggaa accttgcgcc
      661 gactcaaggc aggaaccctc ggcgtaaccg aacaagatga aagccgggtg acccttgcgc
      721 cgatcttaag gaaagaagac ggcctcatcg actggcaacg cccggcgata gaaattgagc
      781 gccgcattcg tggcttcgac ccttggccgg gaagtttcac gcatttcgcc ggtaagctgc
      841 ttaagataca tcgagccaca atagtcggca ctgagcggcg cggcggcccc ggtgaaatca
      901 tccgtgcaga tgcagccggg ttttggctgg caacttcatc gggggtattg agcctcgaag
      961 aagtgcagca agaaaataag aagcggctgg cgggcatcga atttatcaag ggcgcgagaa
     1021 ttaaacccgg tgaccgcctt gaatgatgga aagaacgtcc gccaaatcgc cggcgagatt
     1081 ctgcatagag tcgagagcca gaagtcgtat gccgacctac tgctcgatca ccatttgcgc
     1141 agcgatgatt tcaatgacgc agaccgttcg ctgttaaccg aattgactta cgggacgctt
     1201 cgctggcgcg gtaagatcga cgcccagctt ttgccatttc tcaatcgttc gttggccgaa
     1261 actgattcgt tcaatcgtaa cttacttcgc gttaccctgt atcaattgct cttcctcgac
     1321 aggattcctg cctatgccgc agtcaatgaa gctgtcgaga tcgccaaagc gacacagccc
     1381 ggaagcgcca gcttcgtcaa tggcgtcctg cgcagttttc tcagacacct ggcgaagggt
     1441 ggcatgccca cggacaccca gagttcagtt gcgaatctag ccgatgaata ttcgcatcct
     1501 caatggctgg tcaatcgctg gcgggagtac tttggcgatg agcaagcgaa agcattaatg
     1561 cttgcgagca atcaaaaaac gccgctggtg gtgcggacca atttgggtaa gaccagtcgt
     1621 aaagaattgt tagcgtgctg gcacgaacag gacatcgcag ccgaagccag cgcagtctcg
     1681 ccgcaagcca tccgtttgcc ttcgggttta agcattgaaa tgctgccggg ctttaacaat
     1741 ggattgtttc aagtgcaaag cgaagcgtcg caacttgtat catacctggt cggcccggcg
     1801 tcaggagacc tggttctcga cgcatgcgct gcgccgggcg gcaaagccac ccatctcgcc
     1861 gagttgattg gcgatcaagg taacatcgtg gctatcgata cttcggctcg cggcgtcgag
     1921 agaatcgagc agaatgctca gcgcttagga ctggcaacta tacaagccga gtgcgccaac
     1981 gccagcaagg ctttaccaaa ccaccttgcc ggtccctacg attgcatcct cgttgacgcg
     2041 ccatgcagcg gtcttggtac tttgcgatct cacccggaaa tcaaatggca gcgcgatgac
     2101 agcgacatcg cacgtctagc cgcgctccaa gcacgcattg tacagtccgt ggcgcaatat
     2161 ttaaaagtgg gcggggtgct ggtctattcc acctgtacct tgacggtcga tgagaatgag
     2221 cgagtagttg ataacttttt gcgagaaaac agtcggtttg agttggttga tgccgcgcgc
     2281 tatcttcccg aagcggcgcg ccacatggtc catggtaaat attttcaagc gctgccccag
     2341 cgtgatgata ctgacgggtt tttcgccgcg cgtatgagaa aggtgactta gatgcccggg
     2401 ataaaaattg ctccgtcaat actgtcggcc gacttcagcc ggttgaaaga cgaaatccaa
     2461 gcggtcgaat cggccggcgc agactggatt catgtcgatg ttatggacgg tcacttcgtg
     2521 cccaatatta ctatcggtcc ggtggtagtc gagtgggtgc gcaaagtgac gacgatcgcc
     2581 gtcgatgttc atctcatgat caccgatccc gacaaatacg cgccggaatt catcaaagcc
     2641 ggcgccgact ggttgtcgat tcatcccgat acttgcaccg atcccaacac tacgttgaga
     2701 aaaatccgca atctcggcgc caaggcatcg atcgcggtca atccagatgt cccgctcagc
     2761 aaagtcgttg gctgctttga caatgtcgat atgattctga tgatgaccgt atttcccgga
     2821 tttggcgggc aggcgttcat tgccgatgtc ctgaagaaaa tcaccgaagt caggaaggta
     2881 atcgacgccg gcaggccggg tgtgctcatc gaagtagacg gcggcatcaa aaccgataac
     2941 atcgcggcgg tggtccaagc cggtggcgac gtcatcgttt ccggctctgg gatcttcaaa
     3001 acccccagtt acgccgagac tattcgccaa atgcgccagg ccgtggccta accaaacctt
     3061 cttgccagcc agttgcaaat ttcgtatcga aattccaaac gcttcgcttt attcttgcaa
     3121 tatagaattt gtaattttgt aatctggaat cgaccggcgg tcgagcttgt tttccctctg
     3181 cggtgtgtta gtttgtccgg gtcggggcgt ggctcagctt ggtagagcgc acggttcggg
     3241 tccgtgaagt cggtggttca aatccactcg ccccgaccat ttcttttctt ccaagttatt
     3301 cacaagttga ctgtctttta tatcccgctg ccggtagggt ttcgggaaag gtttcggttt
     3361 acgtctcaaa aagccgcttt aactactcat ccgaatcgtt cttgacgtag cggacatagt
     3421 gcttttcaat catccctacc gatgctccac aatattccac gagccatttg atgttcaccg
     3481 catgcgctaa tgcgtccgat atgaatgtgt gccacgttgc gtagaatttc cggggtctaa
     3541 caccacaagt tctcaaagct cgataccagt atttggcccg ccacttgtct tcgtctatcg
     3601 gtttgccttg gtggttatta aatacgaaat cagtctgtgt tacgtgtaag ggcttaagaa
     3661 agatcaacac tgctgcaagc ggctccgatc ttgttatttc tcgctctcac gcagcagttt
     3721 tagtgggatt gtggcgcttg agattcacat cgccccatcg aagggctatc atctcgcttg
     3781 gtcgagcgcc ggtgccgaac tggccgagac gaaaggtaag tagaagggca cttctccctg
     3841 acatgcacaa taatcgtatc acgctcttca gccgtgaacg gatcgggcat gggtgaaact
     3901 cctctggctg actaaggttg taagagtggt ctttctctcc gatcccatgt ttcagcgcgt
     3961 ctcgcatcat tgcccggaag gtaccgtcga tgacactcct aaaggtaagt ggccgtcaca
     4021 ggtaacgttg gcgaaatctc cccgtgatgg ggccatcgcg agaacatcgg ctcgcctgtt
     4081 gagagcctcc ttcatagaaa aactgttgta gccaactcca tcgaccatca gcccaccggg
     4141 actgagcatg gcgatgcctc atgatccctg acgtgattga gcatgtcgaa ttgccaagca
     4201 ttcgcaccta gctcattggc aatttccaac atcacgttgt ccagattccc caagtgagac
     4261 gattgtggac gcgtatgcgt tctcagggta tggaccaact cctaaagtgc gaggagtatt
     4321 cggggatccc aaggcacgca ttgttac
//
LOCUS       DASQKS010000430         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2766467, whole genome shotgun sequence.
ACCESSION   DASQKS010000430 DASQKS010000000
VERSION     DASQKS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766467"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VE170_11065"
     CDS             <1..985
                     /locus_tag="VE170_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ16019.1"
                     /translation="LTYRNLLQYPMVMGAMNVCDASTMRGCVLPMSHIVGPVVCNELA
                     EKGYTLVIFDQINPVTLLEGIQKYRVNVFESVPIVFQLLLGVKNLASYDTSSMKIAAM
                     MGTSIPMPLLRAFQTAQPHIKVIQGYGLTETSPMITLVEPEKAESKMGSIGRAVPGVE
                     VRIVDDDDKDVPVGEAGEIITRGPHIMKGYFRRADATAQRIRNGWLYTGDVGKVEADG
                     YYYHLGRRDDMIITGGLNVYPAEVENLIYTYPGVQETIVFAIPDAKRGQVIGAAVVPR
                     AGVTLVEKELLAFLRANLANFKVPDRVVFRESLPRTSSGKTIREAATLLEK"
     gene            1133..1846
                     /locus_tag="VE170_11070"
     CDS             1133..1846
                     /locus_tag="VE170_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HYJ16020.1"
                     /translation="MWPAAPAELPALERTVHVWAVPLDDARVDVDGGRELLSPDERER
                     AARFHFEQHRRRFLLAHIALHWILSGYLKIPPARLYFELGSNGKPKLPPSLANSGFEF
                     NLSHSNDMALLAINLIGEVGVDIEYVKPGFKFEEIAESFFTAREVAALRGLPSELQRQ
                     AFFKCWTCKEAFLKAKGTGLSGRLDEVEIALSASDEVRIRADVAGWSLAQLNPIDAYE
                     AALVVASPPVPICCFRWQP"
     gene            2123..2917
                     /locus_tag="VE170_11075"
     CDS             2123..2917
                     /locus_tag="VE170_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HYJ16021.1"
                     /translation="MPCPYTLGFMLTEALKNRYLIATGVLFPVALMLILFVSGHENLS
                     LPWMQSSTSTDPSGSDAIAQMREILTKERRQLAQSEREQSTLRAEVIRQRKLLQEGQL
                     AKEQVQQTEQLFVAALKRVHEMRYTVTETDIAITEAILGEKVLRMPALPIGGFSQTAE
                     LARFNGPFKWSIAEAPRVEKFFSQTFGRRLPITALGQSATHNRLRVDHRDSMDVGLHP
                     DSGEGRTLVGYLRKSGIPFLAFRGIVPGAATGPHIHIGRPSPRLVK"
     gene            3108..3287
                     /locus_tag="VE170_11080"
     CDS             3108..3287
                     /locus_tag="VE170_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16022.1"
                     /translation="MKKFLREEHGQGTVEYTLIVLVVALIFWMGVGTTDIGTSFTNGW
                     SKISDCVFSPFSCSP"
     gene            3630..4379
                     /locus_tag="VE170_11085"
     CDS             3630..4379
                     /locus_tag="VE170_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16023.1"
                     /translation="MKICRRATLLIFAVTALLAPVLGLSQGTPRGFRSSATDSELSRS
                     RSEVIQKTKETRAGAETLLALHEAEVKRITAEYERRRELYHQGFISRSELIQAQDAVA
                     QAVLRLEEDKRFIIETDTALTEFAMRDELLRLPGLVLGGYSETNTLLRFNGAAGFTLA
                     EVDKIGRFFLQSFGRGMPISALGQTATHERMKFDHRNAIDVALHPESAEGKSVLNYLR
                     QAGIPFTAFRGAVAGAATGAHIHIGKPSPRL"
BASE COUNT          988 a         1248 c         1293 g         1035 t
ORIGIN      
        1 gctaacctat cgcaatttgc tccagtatcc gatggtgatg ggcgcgatga acgtctgtga
       61 tgcctcgacg atgcgcggct gcgtgctgcc gatgtcgcat atcgtcgggc cggtggtgtg
      121 taacgagttg gcggaaaaag gctacacgct ggtgatcttc gatcaaatca atccggttac
      181 tttgctcgaa ggcattcaaa aataccgggt caatgttttc gaaagcgtgc ccatcgtgtt
      241 tcagctattg ctcggcgtga agaatctcgc cagctacgat accagcagca tgaagatcgc
      301 cgcgatgatg ggcacgagta ttccgatgcc gctgctgcgc gcgtttcaaa ctgcccagcc
      361 gcatatcaaa gtgattcaag gttacggtct gacggaaact tcgccgatga tcacgctggt
      421 cgagccggaa aaagcggaat ccaagatggg cagcatcggc cgcgccgtgc cgggcgtgga
      481 agtgcgcatc gtcgatgacg acgacaagga cgtgccggtc ggcgaagcgg gagaaatcat
      541 cacccgcggc ccccatatca tgaaaggtta ttttcgcagg gccgatgcta cggcgcaacg
      601 aatccgcaac ggctggctct ataccggcga tgtcggcaag gtcgaagccg acggatatta
      661 ttatcatctc ggccggcgcg acgacatgat catcaccggc ggtttgaacg tctatcctgc
      721 cgaagtggag aacttaattt acacttatcc tggcgtgcag gagaccatcg tctttgccat
      781 cccggatgcc aagcgcggcc aagtgatcgg agccgccgtc gtgccgcgag cgggagtgac
      841 gcttgtcgag aaagagctgc tggcctttct gcgcgccaac ctggctaatt tcaaagtacc
      901 cgaccgcgtc gtcttccgcg aatcgctgcc gcgcacatcc tcgggcaaga cgatccgcga
      961 ggcggcgaca ctgctggaga aatagcacgg ggttcctact atgggcacga ggcggatttc
     1021 tcccgcagca ccgccaggtg atcggcgttg ccggtcgctt aatatcttcg tgcccttacg
     1081 ggagaattgc caaatccgaa gtctgcatcc tccacggtga ttgttaatga ccatgtggcc
     1141 ggcagcgccc gccgaacttc cggcgctcga acggactgtc catgtttggg cggtgccgtt
     1201 agatgacgcg cgcgtcgatg tcgacggcgg gcgggagttg ctgtcgccgg acgagcgtga
     1261 acgcgcggcg cgctttcact tcgagcagca ccgacggcgc tttttgcttg cccatatcgc
     1321 tctgcattgg attctcagcg gctatttaaa aattcccccc gcgcggttgt atttcgagct
     1381 tggcagcaac ggcaagccca aattaccacc gtcactggcc aatagtggct ttgagttcaa
     1441 tctctcccat tccaacgaca tggctttgct cgcgattaac cttatcggcg aggtcggtgt
     1501 cgatatcgag tacgtcaagc ccggcttcaa gttcgaggaa atcgccgaga gcttttttac
     1561 ggcgcgagaa gtcgcggcgc tgcgcggact tccatccgag ctgcagcgcc aggcgttttt
     1621 caaatgttgg acctgtaaag aggcctttct taaagccaaa ggcacggggc tatccggcag
     1681 gctagatgag gtggaaatag cacttagcgc ttccgacgaa gtgaggattc gagctgacgt
     1741 ggctggctgg tccctcgccc aattgaatcc catcgacgct tacgaagcgg cactcgtggt
     1801 ggccagtccg ccggtgccga tctgctgctt tcgttggcag ccgtaataat ttgtccggtc
     1861 ggtcggaacg acactgtcta acgcttgcgg gcctgcatcg caaaagatcg acgaaggaaa
     1921 acgacgaaag ccatgaagcg gtgattcaac gcttcgattc ggttgttaat agttcggcca
     1981 gcattcaaga gcgggcgcgg gaagaggcat agactcctct tggcaaaggt gcatcctccc
     2041 gccccgatac aattaggttg acgttaactc ccgtcaacat cacaattgct acaccggctg
     2101 gcaatcttga cttgacccgt ttatgccgtg cccttacact cttggcttca tgctcaccga
     2161 ggctttaaag aatcgttact tgatcgccac cggggtgttg tttccagtgg cgctgatgtt
     2221 gatcctgttc gtcagcggac atgagaatct tagtctgcct tggatgcaga gcagtacaag
     2281 cactgatccg tcgggttccg atgccattgc ccagatgaga gaaattctta ccaaggagcg
     2341 gcgccagctc gcccagtccg agcgcgaaca atcgacgctt cgtgccgaag ttattcgcca
     2401 gcgcaagctg ttgcaggaag gccaacttgc caaggaacag gtacaacaga cggagcagtt
     2461 gttcgtggcg gcgctcaagc gggttcatga gatgcgctac acggtgaccg aaacggatat
     2521 cgctattaca gaagcgattc ttggcgagaa ggtgctgcgc atgccggcgt tgccaattgg
     2581 tggatttagt cagaccgcgg agttggcccg cttcaacggc cccttcaaat ggtcgatcgc
     2641 cgaagcgcca agggtggaaa aattcttctc ccaaaccttc ggccgccgac tgccgataac
     2701 ggcgttgggg cagtcagcta ctcataatcg gttaagggtt gatcaccgcg attcgatgga
     2761 tgtcggactc catcctgaca gcggcgaggg caggacgctg gtcggttatc tacgcaagtc
     2821 cggcattccg tttctagctt ttcgcggcat agttcccggc gccgctactg gaccacatat
     2881 ccatataggc cgaccctctc cccggctcgt caagtgaacc gctttctttg tgtgcgggca
     2941 actccgcccg tcaacgttgt cattcctggc aaacgggaat tttggctcat gacttccctg
     3001 ctttttccgg ctacgggcaa tcatgatgcc ggcatcttga ttgctttgct gcggtctggg
     3061 actgaaaaac aacttagaat ttcactctaa ggggcagcat gcgtccaatg aaaaaattct
     3121 tgcgggaaga gcatggccag ggaactgtgg agtacacctt gattgtgctc gtcgtcgcac
     3181 tgatattctg gatgggtgtg ggcactaccg atatcggcac tagcttcact aacggttggt
     3241 cgaaaatttc cgattgtgtc ttctcgccat tttcctgcag tccctagttt ggctaacggt
     3301 gctggcgtca aaacaatttc ttactgggtt tcgatgccaa ccgggtggca aagtaatagc
     3361 aagtgcattg actctgaagt ccgttacaaa gaataaacgc cattcgagat acgccggcaa
     3421 tagccggtgc gatctgaaat tcacaacaag cagcgtttat ggctggcgac tcatacggtc
     3481 ccgcgagtgg acaaatgttc gctttttgat aaaccctctg atgcagtaaa acaacttgcc
     3541 cgcggcgatt aaaggcttcc ttacctagtg cttgaccgcc agtcgtagac ttcatagact
     3601 tacgggcagt tttgagttgt gtcagcgcta tgaaaatctg ccgacgagcg acgctgctaa
     3661 tatttgctgt gacagcacta ttggcacccg tgctgggctt gagccagggc acgccaagag
     3721 gcttccgttc cagtgcgacc gactccgaac tctcgcggtc gcgctccgag gtgattcaaa
     3781 aaaccaagga gacccgcgcc ggcgccgaaa cgctgttggc gttgcacgaa gctgaagtta
     3841 aacgcatcac cgcggaatac gagcgccggc gcgaacttta ccaccagggg ttcatttccc
     3901 gtagcgagtt gattcaggcg caggatgcgg tggcgcaagc cgtgttgcgg ctcgaggaag
     3961 acaaacgctt cattatcgaa accgatacag ctctcactga atttgccatg cgcgatgaat
     4021 tgctgcggct ccccggtcta gtcttgggcg ggtatagtga gaccaatact ttattgcgtt
     4081 tcaatggagc ggcgggattt actttggcgg aggtcgataa aatcggcaga ttttttttgc
     4141 aatcctttgg ccgcggtatg cctatcagcg cgcttggcca gacggcgacc cacgagcgca
     4201 tgaaattcga tcatcgcaat gcgatcgatg tggcgctcca ccctgaaagc gccgaaggca
     4261 agtcggtgct caactactta cgtcaggcgg gtattccatt tactgcattt cgcggcgcag
     4321 tcgccggtgc cgccactggg gcgcatattc atatcggcaa accatccccg cggttgtaga
     4381 ggagatctgc aacgaattcg ggctgagctt tccgccgcag ttgcagctgc gccgggtttt
     4441 agttaaatca ctgaaccatg gcacagccaa acattcccat gcccgcttcg acagtcgttc
     4501 tagcgcgccc cagtgaggac ggcgcattcg aaatctttat gaaccggcgc ccggaaaaaa
     4561 tggg
//
LOCUS       DASQKS010000431         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1496777, whole genome shotgun sequence.
ACCESSION   DASQKS010000431 DASQKS010000000
VERSION     DASQKS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496777"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VE170_11090"
     CDS             <1..383
                     /locus_tag="VE170_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16024.1"
                     /translation="MIEGVTLTTTTTYVQKHEEEARSLIMALIDGIHFYKTNKADTLK
                     ILQKHCSELLKMHGDEEWNCFYENQSESLESAPYPSIEAIQNVFALGVKRNPEISEFN
                     PLALWDLHYVKEIDDIGYIRKLYN"
     gene            646..2094
                     /locus_tag="VE170_11095"
     CDS             646..2094
                     /locus_tag="VE170_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HYJ16025.1"
                     /translation="MEEVRIVDTTLRDGDISLWAYGMTTGMMLPALRHMDEAGFDSME
                     FFLSLRFKKFVREHKEDPWDWLRLGTKEIKKTRLRYHGGLHSGFEFIPECIRRLVIST
                     VAKYGIDLTRTSNAWNNFALLGAEAIELKKLGMDTVMNLIYAISPRHTDEYYAQKARD
                     AAALQPYRLCFKDVGGMLTPERTRTLVRLIKQNIGTVPLEFHAHCNNGLAPFNLLEAV
                     KEGVRIVHTAIPPLANGSSQPSIFNVVDNLRALGYQPLVDTTVLPPVREHFSRIAAAE
                     NLPTGAPREYDQAWYGHQVPGGMISNLRHQLKLMGKEEKLAAVLDEIAQVRAELGYPI
                     MVTPFAQFVGSQAAINVIVGERYREATDPVIQYALGQWGKEGADYMDPVVKAKILDRP
                     RAREIAKMTHPEPSLAEVRKQYGGAHLSDEELLLRYYAGPEFVDGLRNAPPRRSYVNG
                     HKPLLRLIEELGKKKLAHVYIRKGDFSLTIQK"
     gene            2153..3175
                     /locus_tag="VE170_11100"
     CDS             2153..3175
                     /locus_tag="VE170_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HYJ16026.1"
                     /translation="MAEKDQQYTEHWGREGFYGDGINVMRAHHVPDFVQVSGPHAPHR
                     LNINDVALPDRNDPQELPMPIFVSRSGAQLSVSGRRRPMPFVISNVEADEVHFIQSGQ
                     ARFATAYGWIEAVPGDFVFIPRAVPYQIDGIEGATLSIIVAIPGAVKFDGAPKFEPRI
                     TVPMVESSTPLAGETTVLVKSFDGITRYVKPYSPLAMVSVNNGNNPVWKINLTDIPPN
                     EVGHPTQFAASPHNDELLYTLSARKRRRPPIHYNVDYDEMIFYFAGPGAWGAVKEAGT
                     LTWVPKGVAHHGPTEDVPEGYLAWMLESRPTLRLTDAGLKVAQLMETDLYGPHPAKSS
                     VRRLGF"
     gene            complement(3249..4742)
                     /locus_tag="VE170_11105"
     CDS             complement(3249..4742)
                     /locus_tag="VE170_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYJ16027.1"
                     /translation="MAQSNFNPVEATVDDIHAGYKSGRFTCRQVVQAYLDRIDAFDKK
                     GPNLNAMITISADALSEADRLDAAYKSAGPVGPLHGIPVIFKDQADVKGMPTTLGSLL
                     FKNYYPDRDSFVAEKLKKAGAVILGKATLGELGGGDTHGSLFGSTRNPYDTERTVGGS
                     SGGSAATVAANYATLAVGQEGLASIRRPSTWNCIAGMRPSAGLVSRSGVYGCWPELFG
                     SLGPMARTVKDLAILLDAMAGYDAEDPITARGVGNTPDTFTRFLNADGLKGARLGVLR
                     ESIGLNSEPDSEDYRKIQEVFDKSIAELKAGGAEIVDPIVIPKIKELLAKRSGAPGDT
                     DASFKNYYGRSANPPFKTPQDVTQSPDFPKVVKRSRERFERKADAAKHYDSLRAQDEL
                     MTNFLKVMADHKLDAIVYKSVEHQPTLIKDGIAPPFTDQKGAPHLNTFLIYVPTITVP
                     AGFTRDNLPAGVCFIGRPYDDGNLIKYAYAYEQATHHRRPPASTTGL"
     gene            4966..>6089
                     /locus_tag="VE170_11110"
     CDS             4966..>6089
                     /locus_tag="VE170_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ16028.1"
                     /translation="MSHRVAGFGTTIFADINALAAKHGAVNLGQGAPDFDGPPEVLAA
                     AVTAINSAINQYAPGIGMAPVRQAIAQHAQRFYQQQLNADTDVLVTSGATEAMFAAIL
                     GLTDAGDEVIVFEPVYDTYVPNLVMAGVTPQYVPLRGENWSFDPDELARAFNARTKAI
                     VVNTPHNPTGKVFSRDELGLIAELCQKHDVIGITDEVYEHIVYDDASHVRLATLPGMA
                     ERTLTISSLGKTFSVTGWKIGWAMGPAALVQAVNQAHQFITYAVAAPLQAAAATALNL
                     PFGYFENLQANYQSRRDRMLQVLRSAGFTVFKPQGGYFIMIDWRGVAPRHVQDDRQFA
                     RWLIEAIGVACIPASPFYQDSDKHLGKYFARFAVCKKDET"
BASE COUNT         1276 a         1730 c         1818 g         1265 t
ORIGIN      
        1 cgatgatcga gggcgtgacc ctgacgacca ccaccaccta cgtgcagaaa cacgaggagg
       61 aagcccgttc gttgatcatg gcgctgatcg acggcatcca cttctacaag accaacaaag
      121 ccgacacttt gaagatcctg caaaaacact gttcggagct gctcaagatg catggcgacg
      181 aggagtggaa ttgcttctac gaaaaccaat ccgagtcgct ggaaagcgcg ccctatccgt
      241 ccatcgaagc gattcaaaac gtcttcgccc tgggcgtcaa gcgcaatccc gagattagcg
      301 agttcaatcc gctggcgctg tgggatctcc attacgtgaa ggagatcgac gacatcggct
      361 atattcgcaa gctctacaat tgaactgact ggaacaggcg tcgaaccgtt gggccgcaaa
      421 gggcgctcaa agaaatcttg gcgtggcgca agtttggtta aaaatgcgcc tccggtgaat
      481 cgccggcgca ctgcaacttc aattgagttt cgttctggcc caatgattgt cgtgtaccga
      541 tcttcgccag acctaaccgg cgcgtcgcgc taagagcagc ggtaagcgtt tctcgctatc
      601 gaccaacggg cgccggaata catttgattt tcgcgagggg ggcaaatgga agaagttcgc
      661 atcgtcgata caaccttacg ggacggcgat atcagcctgt gggcttacgg catgacgacc
      721 ggaatgatgc tgccggcgct gcgccacatg gacgaagccg gcttcgattc catggaattc
      781 ttcctgagcc tgcgcttcaa gaaatttgtc cgcgagcata aggaagatcc ctgggactgg
      841 ctgcgcctgg gcaccaaaga gatcaagaaa acccggctgc gctatcatgg cggcttgcac
      901 agcggctttg agttcatccc cgaatgcatt cgccgcctgg tgatttcaac cgtggccaaa
      961 tacggcatcg acctgacgcg cacttccaac gcttggaata atttcgcgct gctcggcgcg
     1021 gaagcgatcg aactgaaaaa actcggcatg gatacggtga tgaacctgat ctacgcaatc
     1081 tcgccgcgcc ataccgatga atactatgcg cagaaggcgc gcgacgcggc ggcattgcag
     1141 ccctaccggc tttgtttcaa agatgtcggc ggcatgttga cgcccgagcg cacgcgcaca
     1201 ctagtgcggt tgatcaagca aaacatcggc acggtcccgc tcgagtttca cgcccattgc
     1261 aacaacggct tggcgccgtt caatttgctc gaagcggtca aggaaggcgt gcgcatcgtc
     1321 cacacggcga ttccgccgct ggccaacggt tcttcccagc cgtcgatttt taacgtcgtc
     1381 gacaatctgc gcgccttggg ataccagccg ttggtcgata ccacagtatt accgccggtg
     1441 cgcgagcatt tctcgcgcat cgccgcagcg gagaatcttc ccacgggcgc gccgcgggag
     1501 tacgatcaag cctggtatgg ccatcaggtg cctggcggta tgatttccaa cctccgccac
     1561 caattgaaac tcatgggcaa agaggaaaag cttgccgcgg tgctcgacga aatcgctcaa
     1621 gtgcgcgccg aattgggcta tccgatcatg gttacgccct tcgcccagtt cgtcggcagc
     1681 caagcggcga tcaatgtcat cgtcggcgag cgttaccgcg aagcgaccga tccggtgatt
     1741 caatatgcgc tcggccagtg gggcaaagag ggtgccgact acatggaccc ggtcgtgaaa
     1801 gcgaagattc tcgaccgccc gcgcgctcgg gaaatcgcca agatgacgca tccagagccg
     1861 agcctggctg aagtgcgcaa gcaatatggc ggtgcccact tgtctgatga agaattgctg
     1921 ttgcgctact acgccggtcc cgagttcgtc gatgggttaa gaaatgcgcc gccgcgcagg
     1981 tcttacgtga atggccacaa accgttgcta cgtctgatcg aggagctagg caagaaaaag
     2041 cttgcgcatg tatatattcg caagggcgat ttttctttga caattcaaaa ataaaaattt
     2101 aacgcggcga gctaacattc attatcgtcc gtgtccaatc ctggaggggg ccatggcgga
     2161 gaaagaccag cagtataccg aacattgggg gcgagaagga ttttacggtg atggcatcaa
     2221 cgtcatgcgc gcgcatcatg tgccggattt tgtccaggtc agcggacctc atgcgccgca
     2281 tcggctgaac atcaatgacg ttgcgttgcc cgatcgcaac gatccccagg agctgccaat
     2341 gccgatcttc gtaagccgca gcggcgcgca gctcagcgtc tccggccggc gacgccccat
     2401 gccgtttgtg atttcaaacg tcgaggccga tgaagtgcat ttcatccagt ctggccaagc
     2461 gcgttttgcc accgcttatg gctggatcga agcggtgccg ggcgatttcg tttttatccc
     2521 gagagccgtg ccctaccaga ttgacggcat cgagggagcc acgctgtcga tcattgtcgc
     2581 cattcccggc gccgtgaagt tcgatggcgc gccgaaattc gagcctcgga tcactgttcc
     2641 catggtcgaa agttccacgc cgctggccgg tgaaacgact gtcctggtaa aatcattcga
     2701 cggcatcacg cgctacgtca agccctatag tccgttggca atggtgtcgg ttaacaatgg
     2761 caacaatccc gtctggaaga ttaatctaac ggacataccg ccgaacgaag tcggccaccc
     2821 gactcaattc gccgcctcgc cgcacaacga tgaattgctc tacaccttga gcgcgcgcaa
     2881 gcggcgccga ccgccaatcc attacaacgt tgactacgat gagatgattt tttacttcgc
     2941 cggccccggc gcatggggcg ccgtgaagga ggcggggacc ctcacctggg tgcccaaggg
     3001 cgtcgcccac catggtccaa ccgaggatgt cccggaagga tatcttgcct ggatgctcga
     3061 gtcgcggcca accctgcgcc ttaccgatgc cgggctgaag gttgcgcaac tcatggagac
     3121 cgacttgtat ggtccccatc ctgctaaatc gagcgtgcga cggttgggct tctagagaag
     3181 cggaccctcg aagcagcggg cgcggcgtag cagtcccggc aactgtgctg cggtgataat
     3241 cggcattctc acagccccgt cgtgctcgcc ggcggccggc ggtgatgggt cgcttgttcg
     3301 taggcgtagg cgtacttgat caagttgccg tcgtcgtagg gacggccgat gaaacagact
     3361 cccgccggta aattgtcccg tgtgaagccg gccggtacgg tgatcgtcgg cacgtagatt
     3421 aaaaatgtat tcagatgggg ggcgcctttc tgatcggtga aaggcggcgc gatgccgtct
     3481 ttgatcaagg tcggctggtg ctccaccgac ttatagacaa tcgcgtcgag tttgtggtcg
     3541 gccatgacct tcaaaaagtt cgtcatcagc tcgtcctggg cgcgcaggct gtcgtagtgc
     3601 ttggcggcgt cggccttgcg ctcgaagcgc tctcgagaac gcttgacgac cttggggaag
     3661 tcgggggact gggtcacgtc ctgtggggtt ttgaacggcg gattggcgct gcggccataa
     3721 tagtttttaa acgacgcgtc ggtatcgccg ggggcgccgg agcgcttggc gagtaactct
     3781 ttgatcttcg ggatgacgat gggatcgacg atctcggcgc cgccggcttt gagctcagcg
     3841 atggatttgt caaacacttc ctggatcttt cggtaatctt ccgagtctgg ttctgaattc
     3901 aagccgatgg actcgcgcaa gacgcccaag cgcgcgccct tgagtccgtc ggcattcaaa
     3961 aacctggtaa aggtatcggg cgtgttgccg acgccgcgcg cggtgatcgg atcttccgcg
     4021 tcataaccgg ccatggcgtc caatagaatc gctaagtctt tgaccgtgcg cgccatcggc
     4081 cctagggagc caaatagttc gggccagcag ccgtagacgc cgctgcggct aaccaggccg
     4141 gcggaagggc gcatgccggc gatacagttc caggtcgacg gccgtcggat tgacgctaag
     4201 ccctcctggc cgacggcgag ggtcgcgtaa tttgccgcca ccgtggcggc tgagccgccg
     4261 gaagaaccgc cgacggtgcg ttcggtatcg tacgggttgc gcgtcgaacc aaacagcgaa
     4321 ccatgggtgt cgccgccgcc gagttcgcca agggtggctt tgccgaggat caccgcgccg
     4381 gcttttttca atttttccgc tacgaaagaa tcgcgatccg gatagtaatt tttgaacaac
     4441 agcgagccca aagtcgtcgg catgcctttg acatcggcct ggtctttgaa gatcaccgga
     4501 atgccgtgca gcggccccac cgggccggcg gatttgtagg ctgcatcgag ccggtcggct
     4561 tcgctcaacg catcggcgct gatggtgatc atggcgttca agttcggccc ttttttgtcg
     4621 aaggcgtcga tccggtccaa gtacgcctgc accacttggc ggcaggtgaa tcggccggat
     4681 ttatagccgg catggatgtc gtcgacggtc gcttcgactg gattgaaatt gctctgagcc
     4741 atgatgatct ccttgttact gccttaaata ttgaaatggc gcggcaatgt ccagagacaa
     4801 cttattgggg ttcacgattc tctagcgtca atcgccaggc ttgccatgct caggctatgc
     4861 aaaaagggtt aacccgggtg cgatgaggct gccattgaaa tcgcggcggg ttaatgatta
     4921 ttgcaggcaa ggaattttct cgaggcgaat cagaagatgt caccaatgtc ccatagggtc
     4981 gccggtttcg gcacgacgat atttgccgac attaacgctc tggcggctaa acatggcgcg
     5041 gtcaacctcg gccagggcgc gccggatttc gacgggccgc cggaagtgtt ggcggcggcg
     5101 gtgacggcga tcaatagcgc gatcaatcag tacgctcccg gtatcggcat ggcgccggtg
     5161 cgccaggcga tcgcccagca tgcgcagcgc ttttaccagc agcaattaaa tgccgacacc
     5221 gacgtgctgg tgacctcagg cgcgacggag gcgatgtttg cggcgattct cggtttgacc
     5281 gacgccggcg acgaggtgat tgttttcgag ccggtctatg acacctatgt gccgaacctg
     5341 gtcatggccg gcgtgacgcc gcaatatgtt ccgttgcgcg gcgagaattg gagcttcgat
     5401 ccagacgaac tggccagggc cttcaacgcg cgaaccaagg cgatcgtcgt caacacgccg
     5461 cataatccga cgggtaaagt tttctcgcgc gacgagttgg gattgatcgc cgagctgtgc
     5521 caaaagcacg acgtaatcgg catcaccgac gaagtttacg agcatatcgt ttacgacgat
     5581 gccagccacg tgcggctggc gacgcttccc ggtatggctg agcgcaccct gacgatcagc
     5641 agcctgggca agacgttcag cgtcaccggt tggaaaatcg gttgggccat ggggccggcg
     5701 gcattggtac aggcggtcaa tcaggcgcat cagttcatta cctacgccgt ggccgcgccg
     5761 cttcaagccg cggcggcgac agcgctcaat ttgccattcg gatattttga aaacttgcag
     5821 gcgaattatc agtcccggcg cgatcgcatg ctgcaagtcc tgcgcagcgc cggtttcacg
     5881 gtcttcaagc cccaaggcgg ctacttcatc atgatcgact ggcgcggcgt cgcgccgcgt
     5941 catgtgcaag acgataggca gtttgcccgc tggctgattg aagcgatcgg cgtcgcctgt
     6001 atcccggcga gcccatttta tcaggactcc gacaagcatc tgggcaaata tttcgcgcgc
     6061 ttcgcagtgt gcaagaaaga tgaaacctt
//
LOCUS       DASQKS010000432         6099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_656203, whole genome shotgun sequence.
ACCESSION   DASQKS010000432 DASQKS010000000
VERSION     DASQKS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656203"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VE170_11115"
     CDS             <1..608
                     /locus_tag="VE170_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYJ16029.1"
                     /translation="VEKMKNFMKNYSCPIPPALLFILLALSACARSYGDDAIKVTQPT
                     EKVAETVKQKAQATEKREGSQDLVKDEVSVKKSVEGTPIIEQTGEASSYGRGFHGKAT
                     ANGEKFDKNNLTAAHPTLPMGTKATVTNLDNGKSVDVTINDRGPYVKGRDIDVSEGAA
                     KELDMTKSGIAPVKIEAEIPAGEKPASKGNASAPQKKAGQK"
     gene            complement(685..1644)
                     /locus_tag="VE170_11120"
     CDS             complement(685..1644)
                     /locus_tag="VE170_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYJ16030.1"
                     /translation="MQRSIFFIIIVLLCPSFVSGEYLTGAEQLKSAGAEFSWRDVESR
                     SVKILLEYQNGEAKQRIHIGSGFLISRDGLFITAYHVMKYCLEARASRFAAHSDCSES
                     RPRMRYLAQNGPSEYEIEVLSHLNEADSTHGKSSHSPDEIIKQRDFVIARLKAGPPTG
                     FPYWKLKDFVQGKIDLGQPRADFELQPLFPPQKVFIAGYPQQGQFHISHGFLNLRDDK
                     HRGYFSADYELYSPAYLRNAGIALDTKWGMRVENHMSGGVVLDASGAPVGIVVNGSDN
                     IAGVLSIENILENFFSRRRQANAKPAIFLTPNRTPLYLKTTPN"
     gene            2087..2392
                     /locus_tag="VE170_11125"
     CDS             2087..2392
                     /locus_tag="VE170_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYJ16031.1"
                     /translation="MKVGLLARFDAKPGKENELAGYLRAGLARVQQNPGTASWFALRF
                     GPAKFAIFDVFTDEAECQAHLHGIITATLKGKSAELLARPPVIEKVDVIAAKLADFG"
     gene            2475..2639
                     /locus_tag="VE170_11130"
     CDS             2475..2639
                     /locus_tag="VE170_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16032.1"
                     /translation="MAKTKKPKKQTAAKKSTRKRARGISDSQTHQPFEQDVKRRIGHY
                     GGAGEPPIMK"
     gene            3209..3463
                     /locus_tag="VE170_11135"
     CDS             3209..3463
                     /locus_tag="VE170_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16033.1"
                     /translation="MKIIIKVAPRDYSRLRGYIPNESPAYEAMEKATRLDYSLDGVLF
                     AGYSIPCDEEQARIILEIAKRCCPEIASEIEHAMSRAEHV"
     gene            complement(3727..4167)
                     /locus_tag="VE170_11140"
     CDS             complement(3727..4167)
                     /locus_tag="VE170_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ16034.1"
                     /translation="MLIREIMTQGAEVVYLDDTAQEAAAKMRELDVGALPVCDGDRIQ
                     GLVTDRDMAIRLVADGRDPANTKVSDIMTPGVSYCFDDQSVEEAVMLMEAQQIRRLPI
                     LNRDKQLVGMLSLGDLALRTEGTEDEDLADEALKDISEPSELSR"
     gene            4731..5888
                     /locus_tag="VE170_11145"
     CDS             4731..5888
                     /locus_tag="VE170_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ16035.1"
                     /translation="MARKSLIGLILLALIACQRAEAPKAKSESPPPAPAPRLDRDSTP
                     PGSYAPIVDRVAPSVVTVRSARRTRAPRQFPFLNDPRLGELFGGLFGAPGGGREQIRL
                     GLGSGVIVRADGYVLTNQHVIDGADEITIELSNRRSYPAKVIGSDPPSDLAVLKVDAN
                     DIPALAFGNSDEARVGDIVLALGNPLGIGQTVTAGIISAKSRSTGLSDGSFEDFLQTD
                     APINQGNSGGALVNTAGLLLGINSQILSPTGVNIGIGFAVPVNMARNVMEQLISSGKV
                     ERGHLGVGIQQVTPEIAASIGLKEARGVIIAEVSPNSAAARAGIQRGDVVVAFNDTPV
                     VDGNALRNAIAGSKPGTPVKLTILRNGKEQTIEAKLDEYRGPAKAPPAPRE"
BASE COUNT         1484 a         1635 c         1675 g         1305 t
ORIGIN      
        1 gagttgagaa aatgaagaat tttatgaaaa attattcttg ccccataccg cctgcactac
       61 tatttatcct attagcattg tccgcttgcg ctcgaagcta tggcgatgac gccataaaag
      121 ttacccaacc taccgagaaa gttgctgaaa ctgtaaaaca aaaggcccag gctacagaga
      181 agcgcgaggg cagtcaggat ttagtcaaag acgaagtcag cgtaaaaaaa tccgtagagg
      241 gaacaccgat aatcgaacaa accggcgagg catcaagtta cgggcggggg tttcacggta
      301 aagctaccgc caatggggaa aaattcgaca agaataattt aaccgccgcg cacccgacac
      361 tgccgatggg aacaaaagcc accgtgacca atctggacaa cggaaaatcc gtcgatgtga
      421 cgattaacga ccgcgggcct tacgtcaaag gacgcgacat cgatgtatcg gaaggcgcgg
      481 cgaaagaatt agacatgacc aagagcggca ttgctcctgt gaaaatcgag gcggagatac
      541 ccgccggcga aaaacctgca agcaagggca atgcatcggc gcctcagaaa aaggcgggtc
      601 agaaataacc gtcggatgag atcgatcctt attcaacctt gtccggtagg tttcgaccga
      661 gggccgcttt gaaggattgc cgacttaatt cggcgtggtt ttcagataga gcggagttcg
      721 gtttggcgtc aagaaaattg ccggcttggc gttggcttgt cgacgtcggg aaaagaaatt
      781 ttccaggata ttttcgatag aaaggactcc ggcgatgttg tcgctgccgt tgacgacgat
      841 accaaccgga gcgccgctgg cgtcgagcac gacgcctcca ctcatgtgat tctcgacccg
      901 catgccccat ttggtgtcca aggcaatgcc tgcgttacgt agataagccg gtgaatagag
      961 ctcataatcg gcggaaaaat agccgcgatg cttatcgtcg cgcaggttca agaatccgtg
     1021 gctgatgtgg aactgtccct gctgcgggta gccggcgatg aatacttttt gcggtgggaa
     1081 gaggggctgg agttcgaaat ccgctcgcgg ttggcccagg tcgattttgc cttgaacaaa
     1141 atctttaagc ttccaatacg gaaaacccgt tgggggcccg gcctttagcc gggcgatgac
     1201 aaaatcgcgt tgtttgatga tctcgtcggg gctgtggctc gatttgccat gggtcgagtc
     1261 ggcttcgttc agatgggaga gcacttcgat ctcatattcg ctcggcccat tctgcgccag
     1321 atagcgcatg cgcggacgac tttccgagca atcggaatgg gcggcgaagc ggctggcgcg
     1381 cgcttccaaa cagtatttca tcacgtgata tgccgtgatg aacaggccgt cgcgggaaat
     1441 cagaaaccct gagccgatat gaatccgctg tttggcttcg ccgttttgat attcaagcaa
     1501 aattttcacg ctcctcgact caacgtcgcg ccatgaaaat tccgcgccgg ctgatttgag
     1561 ctgctcagcc cccgtcaaat actcgccaga aacgaacgac ggacaaagca acacaatgat
     1621 gatgaaaaaa atcgaacgtt gcacgggaaa ttctccgcag gttaagagac cgtccaaaaa
     1681 aaatcaacta aattcacgca attgaatgaa tttattccgg cgctcaaaac gattggattt
     1741 ttgcgacgga aaaaaatggc acgatcagcc catgtgaccg gctataccta tgattcccca
     1801 taggggatgg ctggatgatc tggcaatttt taatttccgg acccagcgga ttcacgcctc
     1861 gcagtgagcg cacgctggtc tggagattgc agtttattag cctatgggtt tgtcgccgcc
     1921 tcataccgga cggcgaggtc tggagcgcac aatggcttct cgaaatatgg cgaagtattt
     1981 aaaaaaagtc gtggagccgc ccagcgccct gacgcggccc agaccgctat tcaagaagcc
     2041 gcggcctgtc ggggccaagc catcgccgga gccgaaatgg caacgggtga aagtcggttt
     2101 attagctagg ttcgacgcaa aacctggaaa ggaaaacgag ttggcgggtt atctccgagc
     2161 cgggttagcg cgtgtccaac aaaatccggg caccgcatcc tggttcgcgc tccgcttcgg
     2221 gccagcgaaa tttgcaattt tcgatgtctt tacagacgag gccgaatgcc aagcccattt
     2281 gcacggtatc atcacggcga cgctcaaggg aaaatctgcc gagctgttgg cgcggccgcc
     2341 ggtgatcgaa aaagtcgacg taatcgcggc caagctggcc gacttcggct gaagctctac
     2401 gtaagttcgt gcaaactgat ccccgagttt ccggccaagg tttcggcagg cggctactaa
     2461 aaacgggagg ctttatggcg aaaactaaaa aacccaagaa gcaaacagcg gcaaaaaagt
     2521 ccacgagaaa aagagcgcgc ggcatcagcg attcacagac ccatcagccc ttcgagcagg
     2581 acgttaaacg ccgcatcggg cactacggcg gggcgggcga accacccatc atgaagtagg
     2641 ttagggatat taaaatgatt cggatgctta agtccggcga gtatcggttg tattcacgta
     2701 agaaatccaa aaaccggtaa acgtaggaat ctcggcacct tccccagtct gaaggcagcg
     2761 cggagtcatg cacgggccgt gcaatttttt aagcgccatt gatttttaat cgctagcgat
     2821 cgctcgtcac tcagagacga agcgcgcttt gcatataagg aatcgatgac tacatcaaaa
     2881 aaatgggtcc ggaaagtaaa aacggtttcc actgcgccgc ctaaagactt attcaccaaa
     2941 gacgctggaa cgattgccaa gacaatggca aggaagagcg ttagtcccaa aggcatcgga
     3001 tccggtattc ggatgatcca atatttcatc aatcgaggcg gcaagaacct gcctctcacg
     3061 cgcaaacggg agctcgagaa agcaaaaaag ctgctgcaaa ggaaacaagc gaaaaaggcg
     3121 caatgaatgt aaactctcag cgaaaatcgc ggccaatcgc atactcccct tgtgatacaa
     3181 tgtaacgcga agccttggag acgacggcat gaagataatt ataaaagttg cgccgcgaga
     3241 ttacagccga ttgcgaggtt atattcccaa tgagtctccg gcctacgagg cgatggagaa
     3301 ggcgacccgg ctcgattatt cactcgacgg tgtgctgttt gccggatact ctattccgtg
     3361 tgacgaggag caagcgcgga ttatccttga aatcgccaaa cgctgctgtc cggaaatagc
     3421 ctcggaaatt gaacacgcta tgtcgcgcgc tgaacatgtg taaacggatc gctgcgccga
     3481 gatcgtggct tgctattgga gtgggactcc gaacaagcca acagccattc ctaaatgacc
     3541 cgcgcggtac ggacgtcaaa tttcccgctg taagccactt tgagcaactt atggtcgcac
     3601 tgggtatgca gattttttga tcgactttcc cttgccgcgt ttacgttttg gtttaggccg
     3661 cttcggttcg acgatgaccg ccggcgattt ttttgccatg aatagtttct ccaatcaaaa
     3721 ctgcttctat cgactcaact ccgacggctc ggagatgtct ttaagcgctt cgtccgccaa
     3781 gtcttcgtct tctgtgcctt cggtgcgtag ggcgaggtcg ccgagggata acatgcccac
     3841 cagctgtttg tctcggttta ggatcggcag gcgacgaatt tgctgggcct ccatcagcat
     3901 cacggcttct tcgaccgatt gatcgtcgaa acaataactc acaccgggcg tcataatgtc
     3961 gcttacctta gtgttcgcgg gatcgcgccc gtcggcgact aaccgtatcg ccatatccct
     4021 gtccgtgact aatccttgaa ttctgtcgcc gtcgcagacc ggcagcgctc cgacatcgag
     4081 ttcgcgcatt ttcgccgcgg cttcttgggc cgtgtcatcc aagtaaacca cctccgcgcc
     4141 ttgggtcatg atctctctaa ttagcattaa gcacctccgg tttgtttttt tccttcgtcg
     4201 ctcgtttact taatgttgcc gcgcgaccgg taacttggca tgcgagacac gacgcacgct
     4261 ttgccggtga tcgaatgaat ggattcagcc atttcagaat tctcttggcg acgcttgcca
     4321 tgttaatcgg cgcaagaaga atgccagcga ccctccgaga ggcgctggtt actccgtacg
     4381 tagcgagcga ctagctcagg gaggtaataa tttggatcga gcaatgtccc aaatctgggt
     4441 cacatagctt cggacaatct catttgtagt aattttctgg cgtgatcctg ttcgttcaag
     4501 ccgtcggcat gatgcgcgac aaatcgtccc agcgcgtatt ctaacctggc gtagcgactc
     4561 gccaataaca ttgctctggc gatattcacg atgagctttt gcggtgccaa gccaatcacg
     4621 tcctagttac tgtggggcgg atggaatctt gcatgcacaa ggacggaatt taaattgcgc
     4681 ggagcaatca ttcaatccga ggagcgcaca gcttcaatat cggggtggcc atggctagga
     4741 aatctctgat cggcttgatc ttattggcgc tgatcgcttg ccagcgcgcc gaggcgccta
     4801 aggccaagag cgaaagcccg ccgccggcgc cagcgccgcg gctcgatcgc gactccaccc
     4861 cgccggggtc ctatgcgccg atcgtcgatc gggtcgcgcc gtcggtagtg accgttcgct
     4921 cggcccggcg aacgcgcgcg ccgcggcaat tcccatttct caacgatcca cgtttaggcg
     4981 aactcttcgg cggtctgttc ggcgcgccgg gtggcggccg cgaacagatt cgtctgggat
     5041 tgggctccgg cgtcatcgtg cgcgcggacg gctacgttct caccaatcaa cacgtgatcg
     5101 acggagcgga tgaaatcacc attgagttgt ctaaccggcg cagttatccc gcaaaggtga
     5161 tcggcagcga tccgccgagc gatcttgccg tgctcaaggt cgatgccaac gatattccgg
     5221 cgttggcatt cggcaactcg gatgaggcgc gggtcggcga catcgtcctc gcattaggta
     5281 atccattggg tatcggccaa acggttaccg ccgggatcat cagcgccaag agccgatcca
     5341 ccggactgag cgacggcagc ttcgaagatt ttctccagac cgatgcgcca atcaatcagg
     5401 gcaactccgg cggagcgctg gtcaataccg ccgggttgtt gctcggcatc aactcgcaaa
     5461 tactctcacc caccggggtc aatatcggta tcggtttcgc ggtgccggtc aacatggcgc
     5521 gcaacgtcat ggagcaactg atcagcagcg gcaaagtcga gcgcggccat ttaggcgtcg
     5581 gtattcagca ggtgacgccg gaaatcgccg ccagtatcgg actaaaagaa gcccgcggtg
     5641 tgatcatcgc cgaggtcagt cccaatagcg ccgctgcccg ggccggaatc cagcgcggcg
     5701 atgtcgtcgt tgctttcaat gacacaccgg ttgtcgatgg caatgcgttg cgcaatgcca
     5761 tcgcgggcag taagcccggc accccggtca agctgacaat tctacgcaat ggcaaagagc
     5821 aaacgatcga ggccaagttg gacgagtacc gcggccccgc gaaagcgccg ccagcgccgc
     5881 gagaataaaa cacaacttgc tacagcgatc cgacaactgg ccaaagcgat tcaagcacgg
     5941 cgtgccaacc cgcaagcaca agtatccatc ggctagcggc tacggcacgc aaacttttct
     6001 aaattgtata attgccggtt tcaccgccgc gcggcgccgt ttcttgcgcg cggagagtgc
     6061 taacgctctt cttgcccccg gcaagatttt tgataatgt
//
LOCUS       DASQKS010000433         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2353777, whole genome shotgun sequence.
ACCESSION   DASQKS010000433 DASQKS010000000
VERSION     DASQKS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353777"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VE170_11150"
     CDS             complement(<1..747)
                     /locus_tag="VE170_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ16036.1"
                     /translation="MSTQVIMPEPRGSNINWSQLSLVAVAGIAAISILILLGMIIWMS
                     LRTGVPGQPSDYSLKNYVTILADPYTYKVMWTTLYFSAVTIAVAVPLGFIFAWLVERT
                     DMPYKALAMSLLSIGILFPTFLKAMGWVFLLHPRIGVINIFLMQLFGLSTAPLNIATL
                     TGIGWVQGLTLTPLAYVMISAALKSMNPSLVEAASVHGVSQWRTLVRIELPLIWPALF
                     SAVIWMFTVAIAAFDVPGVIGMANNIFTFST"
     gene            complement(951..1991)
                     /locus_tag="VE170_11155"
     CDS             complement(951..1991)
                     /locus_tag="VE170_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16037.1"
                     /translation="MKLIAAILLMLPQFALAQKLEQIVAEAKKEGEVTLVASMSTFGG
                     KKGFTELEALFTKRYGFKAKLNLSGGPSFPQMAARIITEQKAGSKSSTDMYLGSDGTF
                     ATMQEEKALEKVNWAGIFPWVTKEMETTPQESLLVYASFHGIIYNSNMIPKAKAPKSY
                     EDLIDPALSPAWAGKMAIPAYIHWLIRLTPIWGKEKVLNFARKLAPLAGGRLRQGEEE
                     RIVSGEFPIMASTGGPLEAMWKWQTKNAPLVGLPGSSPATSSYYQLAVPKNSVHPNMA
                     KLFIAFMTTREAQSVLEKQDHRSSHLVEGTILANYVKANKIKLQDAKQLSEVFLKEDA
                     GLEEELNKILLK"
     gene            complement(2108..3235)
                     /locus_tag="VE170_11160"
     CDS             complement(2108..3235)
                     /locus_tag="VE170_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16038.1"
                     /translation="MMTQEENELMTRVGAGTPAGGMLRHYWWPVAFTDQVQAKAAPTK
                     VTLLAEDFVLFRDGGGKLGLVALHCSHRGTSLEYGRVEENGIRCCYHGWLWDARGNCL
                     DQPAEPADSTFKERVKHPAFHAQDAGGLIFAYIGPDPAPLLPPYDLLLRDDGCRVVGG
                     GEEFCNWLQRAENSADGTHSIALHAAGYPNMAMKRPTIKWEPTPFGLKETTWVDGVSK
                     PRISHFVFPSHIRHSAARIDEQPRQVIRFRVPKDDYTTNTYYIDFYPHKDGKPTRPEA
                     LRVKGFRKSEPGVYARVQDGWWNLPNREQDRVAQETQGVIADRTKEHLASSDQGILML
                     RKMIRESIDAVAQGKDPFGVMRETDGVITFDSSRDAVEALS"
     gene            complement(3316..>4002)
                     /locus_tag="VE170_11165"
     CDS             complement(3316..>4002)
                     /locus_tag="VE170_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16039.1"
                     /translation="WISKPMEIFPNEGLLIFTSLRGMIYNSNLIPKDKAPKTYEDLVD
                     PKMSPTWAGKLAIPPYVSWLVELSMIWSEDKVKNFTKRLVALSSGRLRYSEEERVVSG
                     EFPVAANMGGATEQMWQWQAKGAPLMAVMGSTPVLPSYFQLAVPKNSVHPHLAKLFVG
                     FMASKEAQAILEKNDFRTSHLVDGTLMSNYLKQNKIKLQEAKDSINFYLQGEDSGLEF
                     KEELTRMLKQ"
BASE COUNT          848 a         1203 c         1078 g          873 t
ORIGIN      
        1 cgtactgaaa gtaaaaatat tgttggccat gccgatcacg cccggcacat cgaaggcggc
       61 gatcgccacg gtaaacatcc aaatcaccgc cgagaacagc gccggccaga tcagcggcag
      121 ctcgatgcgc accagtgtgc gccattggct cacgccgtga acgctcgccg cttcgaccaa
      181 cgacggattc atgctcttga gcgcggcgga aatcatcacg taagccagcg gcgtcagggt
      241 cagtccttgc acccagccaa tgccggtcaa cgtcgcgatg ttgagcggtg cggtgctgag
      301 accgaacagc tgcatgagaa aaatattgat cacgccgatg cgcgggtgga gcagaaatac
      361 ccagcccatg gctttgagaa aagtcggaaa caaaattccg atgctgagca ggctcattgc
      421 cagcgccttg tagggcatgt cggtgcgctc gaccagccag gcgaagataa atcccaacgg
      481 caccgccacg gcgatggtca cggcagagaa atacagcgtc gtccacatca ctttgtacgt
      541 gtacggatcg gccaagatcg tcacatagtt cttgagcgaa tagtccgatg gctgccccgg
      601 cacgccggtg cgcagactca tccaaataat catgcccagc aggatcagaa tggaaatcgc
      661 cgcgatgccg gccaccgcca ccagcgacaa ctgcgaccaa ttgatattcg agccgcgcgg
      721 ctcgggcatg atgacttgag tggacattga aactcgatct ttcgctataa aaacttggat
      781 tctatttgcg tcatacggcg aaacacagaa tccgggcgga gggcgggtcg tgatccgccc
      841 gtacaaccaa ttaccaacaa attacgtcga taccggttct caccggtatg acggatcaca
      901 ggattaacgg ctgccggtca atataatgcg cgttggcagc gaacgcggca tcacttcaag
      961 agaatcttat tcaactcttc ttcgagcccg gcatcttcct tgagaaacac ctcgctcaat
     1021 tgcttggcgt cctgcagctt gatcttgttc gctttgacgt agttagccaa tatcgttccc
     1081 tcgaccaaat gggagctgcg atgatcctgc ttctccagca cgctctgcgc ctcgcgggtg
     1141 gtcatgaagg cgataaacaa tttggccatg ttcgggtgca cggaattttt cggcaccgcg
     1201 agttgatagt acgacgaagt tgccggcgat gagccgggca gtccgaccag cggtgcgttt
     1261 ttggtttgcc atttccacat cgcctccagc ggtccgccgg tgctcgccat gatcggaaat
     1321 tcgccgctga caatgcgctc ttcttcaccc tggcgcagcc ggccgccggc aagcggcgcc
     1381 agtttgcgcg cgaagttgag aactttttct ttgccccaga tcggcgtcaa gcggatcaac
     1441 cagtgaatgt acgcggggat cgccatcttg ccggcccatg ccggcgacag cgccggatcg
     1501 atgagatctt cgtagctctt cggcgccttg gctttcggga tcatgttgga gttgtagatg
     1561 atgccgtgaa acgacgcgta gaccagcaac gattcctgcg gggtggtttc catttccttc
     1621 gttacccagg gaaagattcc cgcccaattc actttctcca gcgctttctc ttcctgcatg
     1681 gtcgcgaagg tgccgtcgga accaagatac atatccgtcg acgacttcga gcccgccttc
     1741 tgctcggtaa tgatgcgcgc cgccatctgc ggaaagctcg gcccgccgct caagttcaat
     1801 ttcgccttga agccatagcg cttggtgaac agcgcttcga gttcggtaaa acctttctta
     1861 ccgccgaacg tcgacatgct cgcgaccagc gtcacttccc cttccttctt agcctcggca
     1921 acaatttgtt caagcttctg cgccaacgcg aattgcggta acattaacag gatggcagct
     1981 ataagtttca tcacagcgat tgctcctttt gagttataac gatgcgaggt cgaggataga
     2041 ggatggaaga tcgcgaaaga atcggaacgc catcgtcgat cccccgtcat cgaccctcga
     2101 cccaatttca cgaaagcgct tccaccgcat cgcgactcga gtcgaacgta atcacgccgt
     2161 cggtctcgcg catcaccccg aagggatctt tgccctgggc caccgcgtca atcgactcgc
     2221 ggatcatttt gcgcagcatg agaatgccct gatcggagct cgctagatgt tccttggtcc
     2281 ggtcggcgat cacgccctga gtttcctgcg ccacccgatc ctgctcgcga ttgggcagat
     2341 tccaccagcc gtcttgcacc cgcgcgtaca cgcccggctc gctcttgcga aagcccttca
     2401 cccgcaacgc ttcgggccgg gtcggcttgc catccttgtg cgggtagaag tcgatgtaat
     2461 aggtgttggt cgtgtagtcg tccttgggca cgcggaaacg gatcacctgg cgcggctgct
     2521 cgtcaatgcg cgccgcggaa tggcggatgt gggaaggaaa aacaaaatgc gagatgcgcg
     2581 gcttcgaaac accgtcgacc caggtggttt ctttcaggcc gaacggcgtc ggctcccatt
     2641 tgatcgtcgg ccgcttcatc gccatgttgg gatagccggc ggcatggagc gcgatggagt
     2701 gcgtaccgtc ggcggaattc tccgcccgtt gcagccagtt gcaaaactct tcgccgccac
     2761 cgaccacccg gcagccatcg tcccgcaata gcaaatcata gggcggcaag agcggtgccg
     2821 ggtcgggtcc gatgtaggcg aaaatcaatc cgccggcatc ctgcgcgtga aaagccggat
     2881 gtttgacccg ctctttgaac gtgctatccg ccggctcggc cggttgatcc aggcaattgc
     2941 cgcgcgcatc ccagagccag ccatggtagc aacagcgaat gccgttctcc tcgacccggc
     3001 catactccaa cgacgtgccg cgatgggaac agtgcagcgc gaccaagccc agcttgccgc
     3061 cgccgtcgcg aaacagcacg aagtcttcgg ccagcaacgt cactttggtc ggcgccgcct
     3121 tggcctgcac ttggtcggta aacgccaccg gccaccaata atgccgcagc attccccccg
     3181 ccggcgtacc cgcgccgacg cgagtcatga gttcgttttc ttcttgagtc atcataatta
     3241 gcctccgttg aggacacact cagagtgacg aggtatttga gagtagagaa tccgtgatcc
     3301 ctcgcttccg ctcttctact gtttcagcat cctggtcagc tcttccttaa actccagtcc
     3361 gctatcctcg ccttggaggt agaagttgat cgaatccttg gcttcctgca acttgatctt
     3421 attttgcttc aaataattcg acatgagcgt gccgtctacc agatgcgatg tgcgaaaatc
     3481 gtttttttcg agtatcgctt gggcttcttt ggaggccatg aaaccgacga agagcttggc
     3541 caaatgcgga tggacggaat ttttcggcac cgctagttga aaataggacg gcaacaccgg
     3601 cgtcgatccc atcaccgcca tcagcggcgc gcccttggcc tgccactgcc acatttgctc
     3661 cgtcgccccg cccatattag cagcgacggg gaattcgccg ctgaccaccc gttcttcttc
     3721 gctatagcgc aatcgacccg agcttaacgc caccaatctt ttggtaaaat tcttgacctt
     3781 atcctcgctc cagatcatcg acagctcgac cagccaggaa acatagggcg ggatggccag
     3841 cttgccggcc caggtcgggc tcatcttggg gtcgaccaga tcctcgtaag ttttcggcgc
     3901 cttgtccttg gggatcaagt ttgagttata aatcatgccg cgcagcgaag taaaaatcag
     3961 cagtccttca ttgggaaaaa tttccattgg tttgctgatc ca
//
LOCUS       DASQKS010000434         9581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2354410, whole genome shotgun sequence.
ACCESSION   DASQKS010000434 DASQKS010000000
VERSION     DASQKS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354410"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VE170_11170"
     CDS             complement(<1..1424)
                     /locus_tag="VE170_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ16040.1"
                     /translation="MKTNQTLTVVGTNAHRVDAIEKVSGKAIYTSDIHLPGMAHARIL
                     RSPVAHARLVKVDASKAKEFPGVIATLTRDDIQGFNFKYGATYKDQSIVAVDKVRYVG
                     DPVAAVLADTPDIAEQALELIEVEYDELPKVTNIEEATAPGAVQVHEGGVARAELRGS
                     TYGAPDRFNGTNICYYLTFGREAADKGFAQSEHIFEDTFRFQKVQHYSLEAHNNIASY
                     DGEKLTVWASCQDPFTLRDHLSGIFGLPLSRIRIIVPYVGGGYGGKLYVKAEPISAAL
                     TWMTRRPVKLQLSINESFKTVTRHPARVTVKTGVSKDGKLIARECQIYMDTGAYADAG
                     PRVTQKAGYRSLGPYNIPYAKVEAYGVYTNTVPAGAFRGFGALQVTWAYESQMDMIAD
                     RLGLDPLDFRLRNLLKKGDLYTKGDTPVDCDMSEGLLKVADRIQWHQKSDQPNRAKGI
                     SCCIKDGGGTYKVAGATVKMSSDG"
     gene            complement(1483..1629)
                     /locus_tag="VE170_11175"
     CDS             complement(1483..1629)
                     /locus_tag="VE170_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16041.1"
                     /translation="MGQAVEITCPSCQKIFVVNPHMLGSGMNFHCPFCDKYFPEKDSP
                     KIRK"
     gene            complement(1826..3271)
                     /locus_tag="VE170_11180"
     CDS             complement(1826..3271)
                     /locus_tag="VE170_11180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16042.1"
                     /translation="MAKDLRTFIADCERQLPGEVIHITKEVNPANYDVTAIIKHLGAQ
                     KKFPIIIFDNPLNLNGKVGPIKLTMNCEISQGKTQIALGMPRDATRPQMAEKCLEMEE
                     HRVPPSIISRKDAPIKQNTMVGDKVDLYQIPIMRHHEMDGGPYIVLSTITKDRKSGIY
                     NLSYHRMEVKSKNSTALYASPRHLWKIFKDYEDNNMEMPVATVLGHHPAFHMGACYSG
                     PFEVSEYDVIGGYMGEPLRLVASETFGDDLLIPADAEVVIEGVLKPGGRVVEGPFGEA
                     PGYLGPQRYTTCVHYEVRAINYRNGAMYQSVITPEGDKPWMDLPREGAYLRRCREAVP
                     GVTAVCKQGRHAHYNVFISMKKMSEGDPARAAAAALTFDHTKNVFVFDEDIDVFNPTD
                     ILWAIATRVQPHLQVNILKPLFRGNFLDPSLRDEIKTSGMIVDATKPTDRPFSPVSKC
                     PDEAMARIKLEDYVPGEVLQHIPMDRTTYWA"
     gene            complement(3375..4211)
                     /gene="purU"
                     /locus_tag="VE170_11185"
     CDS             complement(3375..4211)
                     /gene="purU"
                     /locus_tag="VE170_11185"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HYJ16043.1"
                     /translation="MPPHILRIDCPDEPGLVHKITGVLFNAGYNILNNQEFVDLEAKH
                     FFMRTAFEGATQPDRVVSYLQQILPAESVVKLVATERQSIVILATTEAHCLGDLLIRH
                     YSGELPAQIKAVVANHETLGSLVKSFSIPFHYVSHKDIERAEHEKRLIEVLDLYGPDY
                     LVLAKYMRILTPSFVARYRNRVINIHHSFLPAFIGAKPYHQAYHRGVKIIGATAHIVT
                     DDLDTGPIIAQNVIPVNHTYTATAMVQAGRDAEKIALARAVRLMLDERVFLHGNRSVV
                     FE"
     gene            complement(4303..5130)
                     /locus_tag="VE170_11190"
     CDS             complement(4303..5130)
                     /locus_tag="VE170_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ16044.1"
                     /translation="MKLHLKRTVFTRLVILTVMTAYPVAAALANDQSGLAQLLTAPYR
                     TQANVVRDGHRHPVETLTFFGVKDNSTVVEILPGSRGYYLEILAPFLKAKGLYIAANR
                     DENAAPQYLADHQKFLARLKEDPEVFAKVQVTKFNADRHEIAPAGSADFVLTFRNLHN
                     WIERNEIDGALRAFHKALKPGGVLGVVDHRGRTDIAQEAQMKFGYVREDVAVKLMEKA
                     GFKLAGSSQVNNNPKDTKDYPDGVWTLPPSFRLGDKDRAKYQAIGESDRFTHRYVKQ"
     gene            complement(5353..6312)
                     /locus_tag="VE170_11195"
     CDS             complement(5353..6312)
                     /locus_tag="VE170_11195"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16045.1"
                     /translation="MKLGIGSLLALALALIVDSSTAGAQPKKLRPLRIALPSHSVSST
                     PIYIARSLGIFESMGFEPQILVLEPRAALAALLTGDLDFYTAAGTTGRAALRGVPVRV
                     VMVSANRSDLCVVAAKEFTSVEQLRGKTFGGYTAQASANIVLIELLRRKGLRPDDYKI
                     LNIGTARSPALLSGTVPAAVLTAVEAARLSRQGFRVLARASDDIDLTSGGLGTAIASL
                     QNKRDVMRSTVQAALEGLRIAATQRDKVIPIYMKQFALTAEESAWVYDNVARSWALDG
                     KPTAAANKLDFELTQRDMGLKEPPKMEQIYDFSLLDELAAGKR"
     gene            complement(6362..7333)
                     /locus_tag="VE170_11200"
     CDS             complement(6362..7333)
                     /locus_tag="VE170_11200"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16046.1"
                     /translation="MLRIARYTLIAAALAIGSSWVFTAGTGLAQAKPKLRPLHIALAN
                     NSVTMTAIYVAKHLGIFESYGYDARVLVLEPRAGLAALLAGELDFYTAIGSTARAALR
                     GLPVRVGLVALNRPDFSVVATKDIASFDQLRGRVIGAYTPQGTVNVVLHEMMRRRGFK
                     PDDYKVVNAGTARAAALSGGAVQVAVLNSVETVRMVKAGFHVLGRASDDLELPQSGLG
                     MSLGSMQSRRDFLRPGVQAVLDAIRVIVNQKDKTVPVLMKQLAMSQDEASYVYDAIRP
                     GWALDGRPTANAIKLDAELSQRDMGLKELPRVEQTYDFSILDELTKK"
     gene            complement(7711..9288)
                     /locus_tag="VE170_11205"
     CDS             complement(7711..9288)
                     /locus_tag="VE170_11205"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16047.1"
                     /translation="MKASFRDYIDTLRKSDELLEITKPVDLRDVAALVAQSEKALLFK
                     NLPGYSMSLASGLLQSRNRIALGMGVAYENIADKLGKAMDKPIQPQRVTNAPVKEVQI
                     TGKNINLFDLPVPVFSIMDGGPMITGGVVIAEDPEFGINAGIYRLMLKERNITGIDIV
                     TPNNMRKFAERALAKKQPLPISISIGTHPYELVASTFKANLGVNELTFAGGLRNEALR
                     LADGETVPVPCIADAEIVLEGEILPEGWIHTEGPFAEFNRLMGGLHMNPRVRITSIMH
                     RQEAMYYALQMPWENIWMSAPIYEAAARRVLFEAGVQTVEVNVTPGGCCHWHIVASIK
                     KVPGDGKNAIMALLSIADMKHVTIVDDDIDVFDPVDVEWAVATRVQADRDVVIISNAR
                     SKPLDPSLPLPLLGKVPTTAKMGIDATIPENIPAERYKRIVYFNQDKIKLSDYLGSAA
                     TTAESKAAIKPVESVETLSAKIVAALADSHSFFADLLDKFPTVDYKTVANSVAHLYEH
                     GKIKQDKDGKFELTAVS"
BASE COUNT         1965 a         2732 c         2717 g         2167 t
ORIGIN      
        1 ctgccgtcgg acgacatctt taccgtggcg ccggcgacct tatacgtccc gccgccgtct
       61 ttgatgcaac agcttatccc cttcgcgcga ttcggttggt cgcttttctg gtgccattgg
      121 attcggtcag cgactttgag cagtccttcg ctcatatcgc aatcgacggg cgtatctccc
      181 ttggtgtaga gatcaccctt tttcaggaga tttctcagac gaaaatcgag aggatcgagg
      241 ccgagacgat cggcaatcat gtccatctgc gactcgtacg cccaggtaac ttggagcgcg
      301 ccaaagccgc gaaacgctcc cgccgggacg gtgttggtgt aaaccccgta agcttcaacc
      361 ttggcgtaag gaatattgta gggaccgagc gaccggtagc ctgctttctg cgtcacccgc
      421 ggcccggcat ccgcataggc gccagtgtcc atataaatct ggcattcgcg cgcgattaat
      481 ttgccgtcct tgctcacgcc ggttttcacc gtgaccctcg ccggatggcg ggtcacggtc
      541 ttgaaacttt cgttgatcga aagttgaagc tttaccggcc ggcgcgtcat ccatgtcagc
      601 gctgcagaaa tcggctcggc tttgacataa agcttgccgc cataaccgcc accgacgtag
      661 ggaacaatga tccgaatacg gctcagcggc aagccaaaaa tccccgacaa gtgatcgcgc
      721 aaggtaaaag ggtcctgaca ggacgcccaa acggtcagtt tttcgccgtc ataggaagcg
      781 atattgttgt gcgcctcgag agagtaatgc tggacctttt gaaaacgaaa ggtgtcctcg
      841 aaaatatgct cggactgggc gaagcctttg tcggccgctt ccctgccgaa ggtcaagtaa
      901 tagcagatgt tggttccgtt gaatcgatcc ggcgcgccgt aggtcgagcc gcgtaactca
      961 gcccgcgcga cgccgccttc gtgcacctga accgcgcccg gcgcagtcgc ttcctctata
     1021 ttggtcacct tcggcagctc gtcgtactcg acttcgatca gctccagagc ttgctccgcg
     1081 atgtccggcg tgtcggccaa caccgccgcc actggatcgc cgacgtaacg caccttgtcg
     1141 acggcaacga tggattgatc cttataggtc gcgccatatt taaagttgaa accctggata
     1201 tcatcgcgcg tcaatgtcgc aatgacgccg ggaaattcct tggccttgga ggcgtcgacc
     1261 ttcaccaacc gcgcatgagc gaccggacta cggagtatcc gcgcatgggc catgcccggc
     1321 aagtgaatat cgctggtgta gatcgccttg ccgcttactt tttcgatggc gtcgacccta
     1381 tgcgcattgg tgccgacgac cgtcaaggtt tgattcgtct tcatcgtgcg agctgcaaaa
     1441 aaaagaccac ggtgaaatcg tcaccatggc catggacgga atttatttgc ggattttggg
     1501 actatctttt tcggggaaat atttgtcgca gaacggacaa tggaaattca ttcccgatcc
     1561 cagcatatgc gggttgacga caaaaatttt ctgacaactt ggacaagtga tttcaacggc
     1621 ctggcccatg atttacctct ctttagctat actggatttt tgattttcgc ttttggattg
     1681 gcgcatctga gcaatttggc aatccgcgca acgtgcccaa gattcctttt gcgtcctatg
     1741 cgttcatgcg gccaatccct gagttattcg gggccggtga taccggcccc gactttgtga
     1801 cctttgtggt taaattccct atttcctacg cccaataggt cgtccggtcc atcgggatat
     1861 gctgcaaaac ttcgccgggg acgtaatcct ccaacttgat gcgcgccatc gcttcatccg
     1921 ggcacttgga taccggtgaa aatggccggt cggtcggttt ggtggcatcg acgatcatgc
     1981 ccgaggtctt gatctcgtcg cgcagtgacg gatccaagaa gttgccgcgg aacagcggct
     2041 ttaaaatatt aacctggaga tggggctgta cccgcgtcgc gatcgcccag agaatgtcgg
     2101 tgggattgaa gacgtcaatg tcctcatcga agacaaacac attcttggtg tggtcgaagg
     2161 tcaacgccgc cgcggcggcg cgcgccggat cgccttcgga catcttcttc atcgagatga
     2221 atacattgta atgcgcgtga cgcccctgct tgcagacagc ggtgacgccg ggcacggcct
     2281 cgcggcagcg gcgcaggtag gcgccctcgc gcggcagatc catccacggc ttgtcgcctt
     2341 cgggcgtgat caccgactga tacatggcgc cgttgcgata gttgatggcg cgcacttcgt
     2401 agtgcacgca ggtggtatag cgctgcggtc cgaggtagcc cggcgcttcg ccgaagggac
     2461 cttcgaccac acggccgccc ggtttgagga caccttcgat gacgacctcg gcatcggccg
     2521 ggatcagcaa atcgtcgccg aaggtttccg acgcgaccaa gcgcaagggt tcgcccatgt
     2581 agccgccgat cacgtcatac tcgctcactt caaacggtcc gctgtagcag gcgcccatgt
     2641 ggaatgccgg atgatggccg agcacggttg ccaccggcat ctccatgttg ttgtcctcgt
     2701 aatctttgaa gatcttccac agatggcgcg gcgaagcgta gagcgccgtg gagttcttgg
     2761 acttaacttc catgcggtgg tacgagaggt tatagatccc cgacttgcgg tctttggtga
     2821 tggtcgagag cacaatatac ggcccaccat ccatctcgtg atggcgcatg atcgggatct
     2881 ggtaaagatc gaccttatcg ccgaccatgg tgttctgttt gatcggcgcg tccttccggc
     2941 tgatgattga cggcggcacg cggtgctctt ccatctctaa acatttttcc gccatttgcg
     3001 gccgggtcgc atcccgcggc atacccaagg cgatctgcgt cttaccctga gagatctcgc
     3061 agttcatggt cagtttgatc ggcccgactt tgccgttcaa gtttagcgga ttgtcgaaaa
     3121 tgatgatcgg aaacttcttc tgggcaccga gatgtttgat aatcgcggtc acatcgtagt
     3181 tcgccggatt gacttccttg gtgatgtgaa ttacttcgcc cggcaactgc cgttcacaat
     3241 cggcaataaa agttcgcaaa tccttcgcca tctaggcctc ctgctaagtt atgaaattat
     3301 ttatccaaaa gatttatcgg atggccgaaa acttgtcaat gtagctgatt ataacgggtg
     3361 ccggcaagca gacgttattc gaacaccacc gaccggttgc catgcaggaa aactcgctcg
     3421 tcgagcatga gccgcacggc tctcgccagg gcgatctttt cggcgtcccg gcccgcctgg
     3481 accatcgcgg tggcagtata ggtatgattc accggaatca cgttttgcgc gatgatcggc
     3541 ccggtgtcga ggtcatcggt gacgatatgc gcagtcgcgc cgatgatttt gacaccacgg
     3601 tggtaggcct ggtgataggg cttggcgccg atgaacgctg gcaagaaaga atgatggata
     3661 ttgatcaccc gattacggta acgagcgacg aaggatgggg tcaggatgcg catgtacttg
     3721 gcgagcacga ggtagtcggg tccatacaag tcaagcactt cgattaaccg cttttcgtgt
     3781 tcggcgcgct ctatatcctt gtggcttacg taatgaaacg gaatgctgaa ggatttaacc
     3841 aaagaaccta aggtctcatg gttggcgacc accgctttga tctgcgccgg cagttccccg
     3901 gaataatggc ggatcagaag atcgcctaga caatgagcct cggtggtcgc caggatgacg
     3961 atcgactggc gctcggttgc gaccagctta accaccgact ctgccggcag tatttgttgc
     4021 agataggaaa ccacccggtc cggctgggtc gctccctcga atgccgtacg catgaagaaa
     4081 tgcttggctt cgagatcgac aaactcctga ttattcagaa tattgtaacc ggcgttgaac
     4141 aatactccgg tgatcttgtg aactagacct ggctcgtccg ggcaatcgat acgtaatata
     4201 tgtggcggca cgaataactc tcctgtcact cgtcaatgcc gcgcagtatc gcatggacag
     4261 gggcggagat aaacggacca agacagaacc tgcgcttcag tcttactgct taacgtatcg
     4321 atgggtaaag cggtcgctct ccccgattgc ctggtatttc gctcgatctt tgtcaccgag
     4381 gcgaaagctt ggcggcaagg tccacacgcc gtcgggataa tccttggtgt ccttgggatt
     4441 attattgacc tgggaactgc ccgccagttt gaaaccggct ttttccatca gcttgaccgc
     4501 cacgtcctcc cggacatagc cgaacttcat ctgcgcttct tgagcgatgt cagtgcggcc
     4561 gcgatggtcg acgacgccga gcacgccgcc gggcttgagc gctttgtgaa aggcgcgcag
     4621 cgccccatcg atctcattac gctcgatcca gttgtgcaag ttgcggaagg tcagaacaaa
     4681 gtcggcgctg ccagccggcg cgatctcatg ccggtcggcg ttgaatttgg tcacctgcac
     4741 tttggcgaag acttccggat cttctttgag ccgggcgaga aacttctgat ggtcggctag
     4801 atattgcggc gcggcgttct cgtcgcggtt ggcggcgatg tagaggcctt ttgctttcag
     4861 aaagggcgcg agaatttcca gataataccc gcggctgccc ggcaagatct ccaccacggt
     4921 gctgttgtcc ttgacgccaa agaaggtcag tgtttcgacc ggatggcgat ggccatcgcg
     4981 cacgacattg gcctgggtcc gatagggtgc cgtcaacaat tgggcgaggc cgctttgatc
     5041 attggccagc gcggcggcaa ccgggtacgc cgtcatcacc gtcaaaataa ccagccgcgt
     5101 gaaaacagtt cgtttcaagt gtaacttcat gtcatcctcc gttcacgggt caatgtcccg
     5161 actggaaagt cgattttctc tttgcggtca ttcccgcgca cgcgggaatc caggttaatt
     5221 ctggcacgga atggagagaa cctgcccgct ttcgcgggca ttacggaact tcactccggc
     5281 ttgacggtga gacatttcaa ctatcgccaa aatcgacatt aattccaagt ttaatcgtca
     5341 gcaacaagcc gatcatcttt taccggcggc gagctcgtcg agcagcgaaa aatcataaat
     5401 ctgctccatc ttcggcggtt ccttcagccc catgtcgcgc tgggtcagct cgaagtcgag
     5461 tttgttggca gcggccgtcg gtttgccatc gagggcccag gaacgcgcaa cattgtcgta
     5521 gacccaggcc gattcttcgg cggttaaagc gaactgcttc atgtagatcg gaatcacttt
     5581 gtctctctgt gtcgcagcaa tgcgcagccc ttccaaggcc gcttgcaccg tcgagcgcat
     5641 gacatcgcgc ttgttttgca gactcgcgat ggccgtcccc agtcccccgg atgtcaaatc
     5701 gatatcgtcc gaggcccgcg ccaggacgcg aaaaccttgg cggctaagcc ttgcggcctc
     5761 cacggcggta agaactgccg cgggaaccgt gccgctgagc agcgccgggg acctagcggt
     5821 gccgatattg aggatcttgt aatcatcggg ccgcaagcct ttacgccgca acaattcaat
     5881 cagcacgatg ttcgccgaag cctgagccgt gtagccgccg aaggtcttgc ctcttagctg
     5941 ctcgacgctg gtgaattcct tagccgcgac gacgcacaaa tccgagcgat tggccgaaac
     6001 catcacgacc cgaaccggca cgccgcgcag cgcggcgcgg ccggtggttc ccgccgcggt
     6061 gtaaaaatcc aaatctccgg tcagcaacgc ggcgagcgct gcccggggtt caagcacgag
     6121 gatctgcggt tcgaatccca tgctctcaaa gattcccaga ctgcgcgcga tataaatcgg
     6181 cgtcgagctg actgaatggc tcggcagggc aattcgtagg ggacgcagct tcttcggctg
     6241 cgcgccggcg gtagaactgt cgacgattag cgccagcgcc agtgctagaa gagaacctat
     6301 tccaagtttc atccatgcat ctccggtaga tcaactcatc tctaggacac caaagccggc
     6361 gctacttttt cgtcagttca tccaagatgg aaaaatcgta cgtctgctct acccggggca
     6421 gctctttcag tcccatgtcg cgctgcgaaa gctcggcatc gagtttaatc gcgttggcgg
     6481 ttgggcggcc gtccagcgcc cagccggggc gaatcgcatc atagacatag ctggcttcat
     6541 cttggctcat ggccaactgc ttcatcaaca ccggtacggt cttatccttt tgattgacga
     6601 tcacgcggat ggcatcgagc acggcttgga cacccgggcg taagaagtca cggcggcttt
     6661 gcatactgcc gagcgacatg cccaagccgc tttgcggcag ttccagatca tcgctcgctc
     6721 ggccgaggac atgaaatcca gctttgacca tgcgcacggt ctccacgctg ttgagcaccg
     6781 ctacctgcac cgcgccaccg ctcaacgccg cggcgcgcgc cgtgccggcg ttgacgactt
     6841 tgtagtcatc gggcttgaag ccgcgccggc gcatcatttc atggagcact acattgaccg
     6901 tcccctgcgg cgtgtaagcg ccgatcaccc ggccgcgcaa ctgatcgaag ctggcaatgt
     6961 ccttggttgc gaccaccgag aaatcgggcc ggttgagcgc caccaggccc acgcgcaccg
     7021 gcaaaccgcg cagagcagcg cgggcggtgg aaccaatggc ggtgtagaaa tccaactccc
     7081 ccgccagcaa ggccgccaag ccagcgcgcg gttccaggac caggacgcgg gcgtcataac
     7141 cgtagctttc gaagatgccc agatgcttgg cgacgtagat cgccgtcatg gtcaccgaat
     7201 tgttggccag ggcaatatgc aggggacgaa gtttcggctt cgcctgcgcc aatcccgtgc
     7261 cggcagtgaa tacccacgat gagccgatgg ccagcgctgc ggcgatgagc gtatagcgtg
     7321 cgattcgcaa cattgcgcct ccttcaatgc taatgctaat acctgcgtgg aatcgatgct
     7381 gtcaaggtaa agagtggtcg gcagtcacag aatcatactg actcgctagc cgcggcggtc
     7441 acagtaaaat cgtttttatt ctcggagagt ggtgacttga atcaatcctg atcgccacga
     7501 gcgaatgtgg cgtgtgcgcg atttgatagg ggacgacccc gatcgctttt cctgcgccct
     7561 taacggctgg cgagccgatc tggccctggg gcatggaaac gaaaatgcgc gctgctgaag
     7621 aaggaagctg aaaaataaat ttagctttag gcgcggcgac tgccgacgag aaaggttcga
     7681 ttctcacttc gttcgaatcg accggactaa ctaactgacc gccgtgagtt caaatttgcc
     7741 gtctttgtcc tgcttgatct tgccgtgctc atacaagtgc gccaccgagt tggcgacggt
     7801 cttgtaatcc accgtgggaa atttatccag caggtcggcg aagaaagagt gtgagtcggc
     7861 aagcgccgcg acgatcttgg cgctcaacgt ttccacgctt tccaccggtt taattgccgc
     7921 tttagattct gccgtggtcg cagcgctgcc gaggtagtcg ctcagtttga tcttgtcctg
     7981 attgaaataa acgatccgtt tgtagcgctc ggccggaatg ttttccggaa tcgtcgcatc
     8041 gatgcccatc ttcgccgtcg tcggcacttt gccaagcagc ggcaacggca atgacgggtc
     8101 gaggggcttc gatctggcat tggaaataat caccacgtcg cggtcggcct gcacccgggt
     8161 ggcgactgcc cactcgacgt cgactgggtc aaatacatcg atgtcgtcgt cgacaatggt
     8221 gacatgcttc atatcggcaa tggagaggag cgccatgatg gcgttcttgc catcgcccgg
     8281 caccttcttg atcgacgcga cgatgtgcca gtggcagcag ccgcctggcg tgacgttgac
     8341 ctcgacggtt tgcacaccgg cttcaaacag cacccggcgc gcggcggctt cgtaaatcgg
     8401 cgctgacatc cagatatttt cccagggcat ttgcagggca tagtacatcg cctcttggcg
     8461 atgcatgatc gatgtgatgc gcacccgcgg gttcatgtgc aggccgccca tcagccggtt
     8521 gaattccgcg aacggtcctt cggtgtggat ccagccttcg ggaaggattt ccccttccag
     8581 gacgatctcc gcgtcggcga tacacggtac cggcacggtc tcgccgtcgg ccaagcgcag
     8641 ggcttcgttg cgcagccctc cggcaaaggt caactcgttg acgccgagat tggctttaaa
     8701 agtcgacgcg accagctcgt acggatgggt gccgatggag atcgatatcg gcagcggttg
     8761 ctttttcgcc agggcgcgct cggcgaattt tcgcatgttg ttgggcgtga cgatgtcgat
     8821 gccggtgata ttgcgctcct tgagcatcag gcgataaatg ccggcgttta tgccgaactc
     8881 cggatcctcg gcgatgacta cgccgccggt gatcatcggg ccgccgtcca taatcgagaa
     8941 gaccggcacc ggcagatcga ataaattaat gtttttgccg gtgatttgta cttccttcac
     9001 cggcgcgttg gtgacgcgct gcggctggat gggcttgtcc atcgctttgc ccagcttgtc
     9061 ggcgatgttt tcataggcga cgcccatgcc cagggcaatg cggttacgcg attgcaacaa
     9121 gcccgacgcc aacgacatgg aatagcccgg caggtttttg aacagcaaag ctttttccga
     9181 ctgcgccacc agcgccgcga catcgcgcag gtccaccggc ttggtgattt ccagtagctc
     9241 gtcactcttg cgcagcgtat cgatgtagtc gcgaaagctc gccttcatga aatcactcct
     9301 tgggttcgtt tcgaggagac ttaacatggc gatacggcta attccatcgg gattaatgtt
     9361 ctttacggcg atttccgttc ttcgtatttc gcaatcccac accatgtgtt ataaacgcgg
     9421 gcgaactgaa gcatttcgaa aggcgatcta tggcatcatt cggacttact ttggcaaacc
     9481 gcggcgtgat cataggcgcg gtcacggtac cggaactctt cgacatggcc aaacgcggcg
     9541 aagactccgg cgccttttcc gctgtttggg tcggcgatag c
//
LOCUS       DASQKS010000435         5955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4019678, whole genome shotgun sequence.
ACCESSION   DASQKS010000435 DASQKS010000000
VERSION     DASQKS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019678"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1304
                     /locus_tag="VE170_11210"
     CDS             279..1304
                     /locus_tag="VE170_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16048.1"
                     /translation="MNKCLRALALAGAVIIAGQSAVGAQEYKDKTLTFVVGYSPGGSF
                     DLYARVLARYIGRYLPGNPTRIVENMTGAGGMIAANHLYNRVKPDGLTVGAWASPLVL
                     QHIMGNDAAQFDGRRFGYLGIPAEYDTVCTFNQKSGITKMEDWVHAKRPQKISTIGPG
                     TSTSDIPKLLKVALNLPMDVLDGYKGGADARLAVESGEVDGYCGSWGTVETVWRSAYE
                     SKKIIPVLQATLKPNPKYKQIPVAMSYAKTEEARELLKIADDVHGAQFPFSVPPGMAK
                     DRLELLQRAFIKTFSDADLIAEAKKAQLDIGPVAGPRIAKTLSGLYDLKPATIARLKD
                     ILLPKKN"
     gene            1447..2400
                     /locus_tag="VE170_11215"
     CDS             1447..2400
                     /locus_tag="VE170_11215"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16049.1"
                     /translation="MNIKSLLKFAVILFLSATLQESYGAERPRERIRIAVSSKSIGFF
                     DTWVAHEKGFFRKYGLDSEVITIRPNLSVVAVVAGEVDYTLMTSTVIRSAVKGMPLKI
                     VTIGLKSSFHALVGRGSFKSIAEVKGKKIAISNVGATDELVARALLQKAGLDARRDVT
                     MLSIGASETRFQSLLSGQVDAATLSLPHSVLAKQQGFRFLGSAGDVLHIPFTGFSTTT
                     TKIQRDREQVKRVIQAQMDAMRWIKSQKQEAIQFLRQFFGTDESTAIESYNVYAPLIV
                     DDVRVTAEAVRAILDSEGATAIAWTQVADASLVEELLQKSR"
     gene            complement(2525..3616)
                     /locus_tag="VE170_11220"
     CDS             complement(2525..3616)
                     /locus_tag="VE170_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ16050.1"
                     /translation="MAATSRGATLLGPDKLEIREYPIPDISADGGLVKVEMGGVCGSD
                     FKYLHGGKNGLPYPLILGHEILGRVEKLGKEAAAIHGIEEGDRIIMKGAKGCGRCAHC
                     RRGAARFCKARIGYGGQTTCANPPHLFGGFADYLYLVPDILVTKVSNDLPAEAAVLIG
                     AVMANGYQWAVRQGGVKMGDSVLIQGPGQQGLACTFAAKMAGAAKIIITGLSRDAERL
                     AVAKKFGATRTINVEKENVLEVVRAETDGELVDVAVDVSGNVQAIRTSVECVRTLGTL
                     VVGGLTRDGVDVPLNVNLIVRRQIRFQGAFTTDNDATEIAMNAVRASKFPVEQMVTHT
                     FPLDETEKCIRAVGGEIEGLYPVKALIKP"
     gene            3862..4818
                     /locus_tag="VE170_11225"
     CDS             3862..4818
                     /locus_tag="VE170_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HYJ16051.1"
                     /translation="MAEIVIGIGTSHSPQLSIRAKDWDHLLKKDQTDPRLDYQGLLAK
                     AKPGLAAELTAEKFHQRDEACLKAIKTLGDALQKANADVVVVFGDDQQEQFHDDNMPM
                     FAIYHGKGLPVVKHNNLRPAAWKNAEEQGWADTAPEYETASDLANHLILSLTDAEFDI
                     ARCNKLRAEIGVGHAFSFLYRRILPGTKLPMVPVMVNTYYPPNQPTPKRCYAFGQAVR
                     KAIESWDSTKRVALLASGGLSHVVIDEEIDAMVIDGLKNKKPEVLFQLPRKKLTGGTS
                     EILNWVALAGAMEHRDLKYLEYVTTYRSPAATGCGMGFAYWQ"
     gene            4897..5559
                     /locus_tag="VE170_11230"
     CDS             4897..5559
                     /locus_tag="VE170_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HYJ16052.1"
                     /translation="MAREPIIDRLIKLDTCAVSDALDSLSLKGATWGVRPQWPCAKIA
                     GRAVTMKIKPAGLQQPTQHLGTAPIEAAQPGDVIVIDNGGKLEFSCWGGLLALSAKLK
                     GVSGVVIDGASRDIDEARELEFPVYARGVVPMTARNRVVQESYNQEIQFAGVQCRPGD
                     LVLADGSGIIIVPKEKEQEVVAAAEGIYAKEQEMAAGIREGYSGREMLERLGYEQMLN
                     KK"
     gene            5579..>5955
                     /locus_tag="VE170_11235"
     CDS             5579..>5955
                     /locus_tag="VE170_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16053.1"
                     /translation="MLTHDENSLLTQVGRGTPCGELLRRYWHPVAAAAELTEEKPIRA
                     VKILGEELVVYRDKSGNYGLVGEHCPHRLASLAYGRVDAQGIRCPYHGWKFDGTGKCL
                     EQPAEPRDSTFKDRIKQVAYPVQS"
BASE COUNT         1285 a         1652 c         1819 g         1199 t
ORIGIN      
        1 gcctgctaca tcgcgagata ccgcgtccca acaccgatct tgccgaaaac aaatacacct
       61 tcgtcagata tctcgagcgc accgaggaaa gagcgatgcc gttcgtcggc cgcggttaaa
      121 gcttctggcc cgttcaagaa gtatggccgc aaagaacgca aagggcgcaa gaaagaatcg
      181 ggatgtcggg gcgggttgta aaacgcctcc tgagtagttg cggcccttta tgcctattcg
      241 aggctgaagc atcaagtttt taatcacgag gagattttat gaacaagtgt ttgcgcgcgc
      301 tagcactggc cggcgccgtt atcattgccg gccagagcgc cgtcggagcg caggagtaca
      361 aggacaaaac tttgacgttc gtggtcggct actcgccggg gggatcgttc gatctctacg
      421 cacgggtgct ggcgcgttac atcggccgct acttgccggg caatccgacg cgtatcgtcg
      481 agaacatgac cggcgccggc ggcatgatcg cggccaatca tctgtacaat cgggtcaagc
      541 ccgacggctt gaccgtcggc gcctgggcgt cgcctctggt tttgcaacat atcatgggca
      601 atgacgcggc gcagttcgac ggcaggcgct tcggctatct cggtattcca gccgagtacg
      661 ataccgtctg cacgtttaac caaaagagcg gcatcaccaa gatggaggat tgggtccatg
      721 ccaagcggcc acaaaaaatt tccaccatcg gtcccggcac cagcacgtcc gacattccca
      781 aattgctcaa agtcgcgctc aatctgccga tggacgtgct cgacggctac aagggcggcg
      841 cggacgcccg cctcgcggtg gagtccggtg aggtcgacgg ctactgcggc tcgtggggta
      901 cggtagagac ggtgtggcgc agcgcctacg agagcaagaa aataatcccg gtactgcaag
      961 cgacgcttaa gcccaatccc aaatacaaac aaattccggt ggcgatgagt tacgccaaga
     1021 ccgaagaagc gcgcgagctg ctgaagattg ccgacgatgt ccacggcgcc cagttccctt
     1081 tctcggtacc gccggggatg gcgaaggatc ggctggagct attgcaacga gcgtttatca
     1141 agacatttag cgatgccgat cttatcgccg aagctaaaaa agcccagctc gacatcggtc
     1201 cggtggcggg gccgcggatc gccaagacgc tctccggtct ctacgatctc aaacccgcca
     1261 cgatagcgcg gctgaaagat attttattgc cgaagaagaa ctaggaaagg ttttggcgaa
     1321 gtcaagacaa actggccgcg gtgcggatgg tcggattcga tcgtgatggc gggttgtgta
     1381 aatgcgaaga cgggcgcaac gtgctgcggc cctaaggcgg aaaagctttc gctcaggtgg
     1441 agacttttga atattaaaag cctactcaag tttgcggtga tcctatttct cagtgccacg
     1501 ctgcaggaga gctacggtgc cgagcgtccc cgcgagcgta tccgcatcgc tgtgtcgtcg
     1561 aagtcaattg gctttttcga tacctgggtg gcgcatgaga aaggcttctt tcgcaagtac
     1621 ggtctcgatt ccgaagtgat tacgatccgg cccaatcttt cggtggtcgc tgtggttgcc
     1681 ggcgaggtcg attacacgtt gatgaccagc acggtgatcc gctcggcggt gaagggcatg
     1741 ccgctgaaga tcgttactat cggcctcaag tcgtcgtttc acgctctggt cgggcgcggc
     1801 agtttcaaat cgatagccga ggtcaaaggc aaaaagatcg cgatctccaa tgtcggcgcc
     1861 accgatgaat tggtcgcgag ggcgctgttg cagaaagccg ggctcgatgc gcgccgcgat
     1921 gtcaccatgc tatcgatcgg cgcttccgaa acgcgcttcc agtcgctcct ctccggacag
     1981 gtggacgccg cgaccttgtc actgccgcat tcggtgttgg ccaagcagca gggctttcgc
     2041 tttctcggtt ccgccggcga cgtgttgcat attcctttca ctggattctc caccacgacg
     2101 accaagattc aacgcgaccg cgaacaggtg aagcgggtga tccaggcgca gatggatgcg
     2161 atgcgctgga tcaaaagcca aaaacaagag gcaattcagt tcctgcgcca gttcttcggc
     2221 accgacgagt ccaccgctat cgaatcgtac aacgtgtacg cgccgctgat cgttgatgac
     2281 gtgcgtgtga ccgccgaggc ggtgcgggcg attttagact cggaaggcgc gacggccatt
     2341 gcttggactc aagtggcgga tgcgtcgctg gtggaagaac tgctgcagaa aagccgctga
     2401 tattgcgtgc tatacaagca gaccagccgc tgttcgtgtc acgccgaacg gggcgttggt
     2461 ttgctcaggg atacccacaa agaactgatg tttcggctgc gccaaccgtg aacctcccac
     2521 ccggctacgg tttaatcaac gccttcaccg gatacagccc ctcgatctcg ccgccgacgg
     2581 cccggatgca tttttcggtt tcatcgagcg gaaaagtgtg ggtcaccatt tgctcgacgg
     2641 gaaacttgct cgctcggaca gcgttcatgg cgatctcggt ggcgtcgttg tcggtggtga
     2701 aggcgccctg gaagcggatt tgccggcgca cgatcaaatt tacgttcaac ggcacgtcga
     2761 cgccgtctcg cgtcagcccg ccgacaacca gcgtgccgag ggtgcggacg cattccaccg
     2821 aggtacggat cgcttggacg ttgcctgaaa catcgaccgc gacgtcgacc agttcaccgt
     2881 cggtttctgc gcggacgact tcaaggacat tttctttttc gacgttgatc gtccgcgtgg
     2941 cgccgaactt tttggcaacc gccaagcgct ccgcatcacg gcttaatccc gtgatgataa
     3001 tttttgccgc gcccgccatc tttgccgcga aggtgcaggc gaggccttgt tggccaggcc
     3061 cctgaatcag cacgctgtcg cccattttga cgccgccttg gcgcaccgcc cattggtagc
     3121 cgttggccat gacggcgccg atgagcaccg ccgcttcggc cgggagatcg ttgctgactt
     3181 tggtgacgag aatgtcgggc acgagataaa gatagtcggc gaagccgccg aacagatggg
     3241 gtgggttggc gcaggtggtt tggccgccgt agccgatacg cgctttgcaa aagcgcgccg
     3301 cgccgcgccg gcagtgggcg cagcggccgc agcccttggc gcctttcatg atgatgcggt
     3361 cgccttcttc gatgccgtgg atcgctgccg cttccttgcc gagtttttcg acgcggccca
     3421 aaatttcatg gccgagaatt agcgggtagg gcaggccgtt cttgccacca tgcagatatt
     3481 tgaaatcgga accgcagacg ccgcccattt cgactttgac caggccgccg tcggcgctaa
     3541 tatcggggat gggatattcc ctgatttcca gtttgtccgg tccgagtaac gtcgcgcctc
     3601 ggctggttgc cgccatggag cacctccaag cttttgcgca attgttacgg caagcgcggg
     3661 gcgatcgtca agtcaaagac agtcttgccg catagaatgc aaataagaat caggacgtaa
     3721 ttgcagaaac gcaaaaccat ggacaaaaac gaatccgaca atcgcgttgc ttgacaggga
     3781 cggaagtttg cttgtagtca ttgacacatt ggctactggc aaccgtttaa tggcgcccag
     3841 tggccaagga ggagtaaatt tatggcagag atcgttatcg gcatcggcac atcgcatagt
     3901 ccgcagttga gcatccgcgc caaagactgg gatcatttac tcaaaaaaga tcagacggat
     3961 ccgcgcctcg actaccaagg gttgctggcg aaggcgaagc ccggattggc ggcggagctc
     4021 acagcggaaa aatttcacca gcgcgacgaa gcttgtctaa aggcgattaa aaccttaggc
     4081 gacgcgctgc aaaaggctaa tgccgatgtg gtggtggttt ttggcgacga tcaacaggag
     4141 cagtttcacg acgacaatat gccgatgttc gccatctacc acggcaaggg gctgccggtg
     4201 gtgaagcata acaacctgcg gccggcggca tggaaaaatg ccgaagagca gggctgggcg
     4261 gacaccgcac cggaatacga gaccgcatcc gacttggcta accatttgat ccttagcctc
     4321 accgacgcgg agttcgacat cgcgcgctgc aacaaactgc gcgccgagat cggtgtcggc
     4381 catgcattca gttttcttta tcggcgcatt ttaccgggaa ccaaactgcc gatggtgccg
     4441 gtgatggtga acacttacta tccgcccaac cagccgactc cgaagcgctg ctacgcattc
     4501 ggtcaggcgg tgcgcaaagc catcgagtct tgggatagta ccaagcgcgt cgcgctattg
     4561 gcgtcgggcg ggctgagcca cgtggtgatc gacgaagaaa tcgacgccat ggtgatcgac
     4621 ggcctgaaaa ataagaagcc ggaagtgctg tttcagttgc cgcgaaaaaa attgaccggc
     4681 ggcacctcgg agattctcaa ctgggtggcg ctcgccggcg cgatggagca tcgcgacctg
     4741 aaatatctcg aatatgtcac gacctatcgc tcgccggcgg cgaccggctg cggcatggga
     4801 ttcgcgtact ggcagtaaat agattggttc aaggttccag attcaagagt tcaaagttaa
     4861 acggattcaa gttgctaaat cgcgtctgga gttttaatgg cacgtgaacc gataatcgac
     4921 agactgatca agctcgacac ctgcgcggtg tcggatgcgc tggacagttt gagcctcaag
     4981 ggggcgacct ggggagtgcg gccgcagtgg ccctgtgcaa agatcgccgg ccgggcggtg
     5041 acgatgaaga tcaagccggc cggcttgcag cagccgacgc agcatttagg cacggctccg
     5101 attgaagcag cgcagccggg cgatgttatc gtcatcgata acggcggcaa gctggaattc
     5161 tcctgttggg gcggtttgct ggcgctgtcg gccaagctta agggcgtgag cggcgtggtc
     5221 atcgacggcg cgtcgcgcga catcgacgag gcgcgcgagt tggagtttcc agtctacgcc
     5281 cgcggcgtgg tgccgatgac ggcgcgcaat cgcgtggtcc aggaatccta caatcaagaa
     5341 attcaattcg ccggcgtgca gtgccgtccc ggtgatctgg tgctcgccga cggcagcggc
     5401 atcatcattg ttcccaagga aaaagagcaa gaagtggttg ctgccgccga ggggatttac
     5461 gccaaggaac aggaaatggc cgccggtatc cgcgagggtt attcgggccg cgaaatgttg
     5521 gagcggcttg gatacgagca gatgctcaac aagaagtagc cgctgataaa aatgacctat
     5581 gctgacccat gacgagaaca gcctgctgac tcaagtgggc cggggcacgc cgtgcggcga
     5641 attgttgcgc cgttattggc atccggtcgc cgcagccgcc gaattgaccg aggaaaagcc
     5701 gattcgcgcc gtcaagattc tcggcgagga attggtggtc taccgcgaca agtcgggcaa
     5761 ctacggcttg gtcggcgagc attgtccgca tcgtttggcg tcactcgcct acggccgggt
     5821 cgatgctcaa ggcattcgct gtccctatca tggctggaag ttcgacggca cggggaagtg
     5881 cctggagcag ccggcggaac cccgcgacag taccttcaaa gatcgcatca aacaggtcgc
     5941 ctatcccgtg caatc
//
LOCUS       DASQKS010000436         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5269997, whole genome shotgun sequence.
ACCESSION   DASQKS010000436 DASQKS010000000
VERSION     DASQKS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5269997"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VE170_11240"
     CDS             complement(<1..764)
                     /locus_tag="VE170_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYJ16054.1"
                     /translation="MRVREFLALHAVQQADGDLEQEVHGLTYDSRQAAAGKIFFAVPG
                     EQADGHDYIGEAIKRGAAAVVVSRAGAWPHARATVRVNNVRNAMGLWAAHFYRRPSGS
                     LRLVGVTGTNGKTTLTYLVESILHEAGLTPGVIGTINYRYRGRTLPSHHTTPESVDLQ
                     ELLAEMVRSGVQAVAMEVSSHALAQERVRGLDFDVGVFTNLTRDHLDYHRDMDDYFAA
                     KSRLFNDYLPASVKPHKAAVIYGDDPRGESLTVNCRT"
     gene            complement(770..2719)
                     /locus_tag="VE170_11245"
     CDS             complement(770..2719)
                     /locus_tag="VE170_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HYJ16055.1"
                     /translation="MRRSLKRNRLRMGCVKIVFLLVFLIVGGRAFHLQVFQGDKLMRL
                     GQRQHLKEWIVLPKRGALYDRGSEPLALSLESQSVYARPHRLQNRAEADVKLARILNM
                     KRADVEQKLAAKKPFVWLKRQVSPVEAEQIQALRMDGIGMSLEPTRYYPQGGMAGQLL
                     GFVNRDSEGLEGIELQYNDYIRGAAGSSVSERDALGRRVLVEGVEGLHVPPGSDVHLT
                     LDSPIQHLAEKELAATIQKYRARAGVAIVVDPATGEVLALANYPSFDPNSAAQQSADQ
                     RRNRAVTDSFEPGSTFKTILAAAALEEGVVGKDDLFYCEMGKYHYADRVIHDTHPNGW
                     LPFSKILQVSSNIGFTKVAEKLKKDRYFKYIDKFGFGRLTGLDVPGEVAGLLRKPENW
                     SGIDLATHAFGQGISTTPMQMVMAYAAIANGGILMRPYVTRKIVDPQGGVIVENQPQI
                     VRRVISEKTAKLLASMLRDVTNEGGTGVAAKVDGFEVAGKTGTAQKAERGGYSAKKRV
                     SSFVGFVPAENPRLVALVLVDEPEGNVYGGVVAAPAFRNIAQGALRQLAVAPRDGAVM
                     PSAPAQAETALPRPVKRAGIQLVSITEDAVPDFLGLSMREALEKAQSLRINLKVQGNG
                     YVVKQAPAAGERRPEGQTVVVNLQG"
     gene            complement(2716..3069)
                     /locus_tag="VE170_11250"
     CDS             complement(2716..3069)
                     /locus_tag="VE170_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HYJ16056.1"
                     /translation="MAMATAVELENRRVARPKRAHNSRAAQATRRRRHLGVSLLIAML
                     LGIVLVHVWLRLQVVHMGYVLATASKLQNRLEQENRELKVELATLTTRDRLNEMARRR
                     LGLAAPQKGQVIVLP"
     gene            complement(3073..>3712)
                     /gene="rsmH"
                     /locus_tag="VE170_11255"
     CDS             complement(3073..>3712)
                     /gene="rsmH"
                     /locus_tag="VE170_11255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYJ16057.1"
                     /translation="VILDLGVSSWQIDAAERGFSFRLGGRLDMRMDRRQSLDACRLVN
                     TATVDELMRIFRSYGEEPQARRIAERIAVERKLRPIESTDELARLVEGVKGGRRRDHH
                     PATQVFQALRIAVNQELDQLEHFLDDGYALLQPKRRMVIISFHSLEDRLVKNAFRKWG
                     RACLCPPRALVCQCGWNQKVKLLTKKPLVPSDSETAANPRARSAKLRAVERI"
BASE COUNT          788 a         1158 c          999 g          767 t
ORIGIN      
        1 gtccggcagt taacggtcaa tgactcgcca cgcggatcat cgccgtaaat caccgccgcc
       61 ttatgaggct tcaccgatgc cggcaggtag tcattgaaca agcggctctt ggcggcaaaa
      121 tagtcgtcca tgtcacgatg ataatcgaga tgatcgcgcg tcaagttggt aaacacgccg
      181 acgtcgaaat ccagaccgcg gactctttcc tgggccagag catgggacga aacttccatc
      241 gccaccgcct gcactccgct ccgcaccatc tccgccagca actcctgcaa atcaacggac
      301 tcgggcgtcg tatgatgcga cggcagcgtg cggccgcggt aacggtagtt gatcgtgccg
      361 atgactccgg gtgtcaagcc ggcctcatgc aaaatcgact cgaccaaata ggtcaaagtg
      421 gttttgccat tggtaccggt tacgccgacc agccgtaaac tgccgctcgg gcgccgataa
      481 aagtgcgccg cccataaccc catggcgttt ctgacattgt tgaccctcac cgtggcgcgc
      541 gcgtggggcc aagcaccggc gcgcgacact actaccgcgg cggcgccgcg cttgatcgct
      601 tcgccgatgt agtcatggcc gtcggcctgt tcgcctggca cggcaaagaa aattttcccc
      661 gcagccgctt ggcgcgagtc gtaagttagg ccgtgcactt cctgctccag atcgccgtcc
      721 gcctgttgga ccgcgtgcaa tgccaagaac tcgcgtactc gcatcggcgt catccttgca
      781 gatttaccac caccgtttgg ccttccggcc ggcgttcgcc cgctgcgggt gcctgcttga
      841 caacatagcc gttgccctgc actttcaaat taattcttaa agactgggct ttctccaaag
      901 cctcgcgcat gctcaagccg agaaaatccg gcaccgcatc ttcagtgatg gagaccagtt
      961 gaatgccggc acgcttgact ggacgcggta gcgccgtttc ggcttgcgcc ggcgcgctcg
     1021 gcatcaccgc cccgtcacgc ggcgccaccg cgagttgacg caatgcgccc tgcgcgatat
     1081 tgcggaatgc cggtgcggcg accacgccgc cgtaaacatt tccttccggt tcatcgacta
     1141 agaccaacgc caccaagcgc ggattctccg cggggacgaa gccaacgaac gaactcaccc
     1201 gttttttcgc cgagtaacca ccgcgctcag ccttctgtgc cgtaccggtc ttgccggcga
     1261 cttcgaagcc atcgactttg gccgccacgc cggttccgcc ttcgttggtg acatcacgca
     1321 gcatcgacgc caataacttc gccgtcttct cggaaataac ccggcgaaca atttgcggct
     1381 ggttttcgac tatgacccca ccctgcgggt caacgatttt gcgggttacg taggggcgca
     1441 tgagaatacc gccgttggcg atggccgcat aggccatgac catctgcatc ggcgtcgtcg
     1501 atataccctg accgaatgca tgagttgcca gatcgatacc ggaccagttt tccggtttcc
     1561 gtagcagacc ggcaacctcg ccggggacat cgaggccggt cagccggcca aagccgaatt
     1621 tgtcgatgta tttgaaataa cggtctttct tgagcttctc agcgaccttg gtaaagccga
     1681 tattactcga cacctggaga atttttgaaa atggcagcca gccgttggga tgggtgtcgt
     1741 ggatcacccg gtcggcatag tgatacttgc ccatctcaca gtaaaacaga tcgtctttgc
     1801 cgaccacccc ttcttcgagc gccgcagccg cgagaatagt tttaaatgtc gacccgggtt
     1861 cgaagctgtc ggtcaccgca cgattgcgcc gttggtcggc gctctgttgg gcagcactgt
     1921 tgggatcgaa gcttggataa ttagccaacg ccaagacttc gccggtggca ggatcgacaa
     1981 cgatcgcgac ccctgccctg gcgcggtatt tttgaattgt cgctgccagt tccttttccg
     2041 ccaaatgctg gattggcgaa tccaaggtca gatggacgtc gctgccgggt ggcacgtgta
     2101 aaccttccac accctcgacc aagacccggc gcccaagcgc gtcccgttca gatactgaag
     2161 accccgccgc gccgcgaata tagtcgttgt attgcaactc gataccttcg agcccttcgg
     2221 aatcgcgatt gacgaacccc aacaattgtc cggccatgcc gccctgcggg taatagcgcg
     2281 tcggttccag ggacatgcca atgccgtcca tacggagcgc ttgaatctgt tccgcttcca
     2341 ccggcgagac ctggcgcttg agccaaacaa aaggtttttt ggcggcgagc ttttgctcca
     2401 catcggcgcg tttcatattg agaatacggg cgagctttac atcggcctct gcgcgatttt
     2461 gaagccgatg aggtcgggcg taaaccgact gagactccaa actcaatgcc aatggctcgc
     2521 tgccgcggtc gtataaagca ccgcgcttgg gcagaacgat ccattccttc agatgttgac
     2581 gctggccgag acgcatcaac ttgtcacctt ggaaaacttg caagtgaaat gcgcgcccgc
     2641 cgacaatcaa aaataccaga aggaaaacaa tcttcacaca acccatgcgc aggcgatttc
     2701 gtttaagtga acgtctcatg gtaagacgat gacctgacct ttctgcggcg ctgccagacc
     2761 caaacggcgg cgcgccatct cattgagccg atcccgcgtt gtcaaagtcg ccagctcgac
     2821 cttgagctcg cgattctcct gctccaaacg attctgcaac ttactcgcgg tggcgagcac
     2881 gtatcccata tggacaacct gcaaacgcaa ccaaacatgg accagcacga taccgagcag
     2941 catggcgatg agtaaactca cgcccaaatg gcggcgccgg cgcgtcgcct gagcggcgcg
     3001 gctgttgtgc gcgcgtttgg ggcgcgcgac acgccgattt tcaagttcaa ccgcagttgc
     3061 catagccatg actcagatgc gctcgacggc gcgtaatttg gccgaccggg cgcgcgggtt
     3121 ggccgccgtc tcagagtcgc tcggtactaa cggtttctta gttaaaagtt tcaccttttg
     3181 attccaacca cactggcaaa ccagcgcccg cggcggacat aaacaagcgc gaccccattt
     3241 acgaaaagca ttcttcacta ggcgatcttc caacgaatga aacgaaataa tcaccatgcg
     3301 ccgtttgggc tgcagcaagg cataaccatc gtcgagaaaa tgttccaact gatcgagctc
     3361 ctggttcacg gcgatgcgca gtgcttgaaa cacctgggtc gccggatggt ggtcgcggcg
     3421 ccggccgcct ttgactcctt caaccaagcg cgccagttca tcggtgctct cgatcggccg
     3481 gagctttctt tcgacggcga tccgctcggc tatgcgccgg gcttgcggct cctcaccgta
     3541 agagcgaaag attcgcatca gctcatcgac cgtggccgtg ttaaccagcc ggcaagcatc
     3601 gagagattgc cggcggtcca tgcgcatatc caagcgcccg cccaagcgaa aactaaatcc
     3661 gcgctccgct gcatcgattt gccacgatga aacacctaag tccaaaataa ca
//
LOCUS       DASQKS010000437         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4024434, whole genome shotgun sequence.
ACCESSION   DASQKS010000437 DASQKS010000000
VERSION     DASQKS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4024434"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..2048)
                     /locus_tag="VE170_11260"
     CDS             complement(12..2048)
                     /locus_tag="VE170_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYJ16058.1"
                     /translation="MRIAVDIGGTFTDLVAVDDDGRVSRSKALTTPDDLARGIDDCLK
                     GANVEVGDASFFVHGSTVTINAVLERKGARTGLITTKGFRDVYEIGRGNRPEGYNLFF
                     KRPVPLVPRDLRLEVDERLYATGEVLTPLDETSAAAAIAALKKAGVESVAVCLLHSYA
                     NAAHEQRLGELLRQQFPEAYVSLSHEILREFREYERTSTTALNSYVGPLVRRYLLSLE
                     KMLSAAGFRGTFRVMQSNGGVMSAETAKKMPVTMMESGPVAGVIAAARLGEALGCRHI
                     ISFDMGGTTAKSSLIKDFHPEVTSSYYVGGYVTGHPMMLPVVDIVEVGNGGGSIAWID
                     PAGGLKVGPQSAGAAPGPACYGKGGVEPTVTDANLIAGRIDPEYFLGSGIRLQRDQAA
                     AAIMAKVGKPLGLSLEDAALGILTIANFNMSLSVRAVSVEKGYDPRDCVLVPSGGGGA
                     LHAIAIARELSVPRVIIPPMPAHFSALGMLMADLKHDYVQTFVRELADTSGAQIADAF
                     AVLEKSAGDTLREEGCKPEQMVLRRFLDMRYRGQEYTLPVPLTEELRTLSDFSAIRAR
                     FDQMHQDHYGHSAPKEPVMMVNLRLSALGKFDNQLPLATASSAEDRGERGRRPVIFDT
                     QPVDCPIYLSSGFKSGDQLAGPAVIEELGATILVYPGDTMQVNEHGQLVIEVGS"
     gene            complement(2180..3337)
                     /locus_tag="VE170_11265"
     CDS             complement(2180..3337)
                     /locus_tag="VE170_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYJ16059.1"
                     /translation="MIFGRHSVLKRGCSTWDAAAVPQAEFQARLDGVRREMARRDLDA
                     LVIYGDNYSFADLCYLTNYFPKVRGGIAVVPRQGAISMLLNIGSRDVPFAKTLTWVED
                     VRASNQVGSDGAKMIKEKGFESARIGLADSGHGFPLPQLEDMKSALPDVSWLDCDDSV
                     APLRLAKTARELGAMSEAGRLLKEVCAGAVAFIKPGRKEYEVVADIDRLARDMGAEDI
                     RILAGAQRLNPPSFKQAASVGSHWAVYLAVQHERYWAEAGRTFILSGDNKILSCYDRA
                     RKIVAAMARQLRPNGAVAAIDETARNELGEFYSKAAVYGLANGIGLNQWEAPFLNASD
                     AHEVGATTVGASTLNENMTVALRVIIESEGKIVLYGDSFHVTASGAKSLVA"
     gene            complement(3465..>4337)
                     /locus_tag="VE170_11270"
     CDS             complement(3465..>4337)
                     /locus_tag="VE170_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ16060.1"
                     /translation="WVADVRPFLDFHDRLAKMAGKVGNVGLIGREAMHVGLHDAIVKV
                     LGRTPVNAKDIIETMARCKGPFELERMRRAAAIADAGVSALHEDAQPGLKEYELAAIV
                     EYKMRSLGAEDNFGMVVANNHNQALHPPTDRIVQPGDIIIGEITPCIGGLFVQICRTV
                     VLGEPPPVVREKYAILHKAMGLGMAAAQTGAPANAVAKAINGTFIEYGYAEYCRPPFM
                     RVRGHGLGCGSSAPGSLEDDNKTQLEEGMTFIIHPNQYVPETGYLTLGDTVVMGKIQA
                     ESLMQSSWDLHIKE"
BASE COUNT          887 a         1383 c         1192 g          875 t
ORIGIN      
        1 cgaatcttct cctagctccc cacttcgatc accagttggc cgtgttcgtt cacctgcatg
       61 gtgtcaccgg ggtaaacgag aatagtcgcg cccaactctt cgatcaccgc gggacctgct
      121 aattggtcgc cagatttgaa accgctgctt agataaatcg gacagtcgac gggctgggtg
      181 tcgaagatta ccggccgtcg tcctcgctcg ccgcggtctt cggcagatga cgccgtcgcc
      241 agcggtagtt ggttatcgaa ctttcccaat gcggatagac gcaaattgac catcatcacc
      301 ggctctttag gcgccgagtg accataatga tcttgatgca tttgatcgaa acgcgcgcgg
      361 atcgcgctga agtcgctgag cgtgcgcagt tcttcggtca gcggcaccgg caacgtgtac
      421 tcctggccgc ggtagcgcat gtcgagaaag cgccgcaaga ccatctgttc cggcttgcag
      481 ccctcttcgc gcaaggtatc gccggccgat ttctccaaca ctgcgaacgc gtcggcgatc
      541 tgcgcgccgc tggtgtcggc caactcgcgg acgaaagtct gcacatagtc atgcttcaaa
      601 tctgccatca gcatgccgag ggcggaaaaa tgcgccggca tcggcgggat gatcacccgc
      661 ggcaccgaca gctcgcgagc gatggcgatt gcgtgcagcg cgccgccgcc gccgctcggc
      721 accagcacgc aatcgcgcgg gtcgtaacct ttctcgacgg aaaccgcgcg caccgacagc
      781 gacatgttga aattggcaat ggtgagaatt cccagcgccg catcttccaa cgacagcccg
      841 agcggcttgc cgacctttgc catgatcgcc gccgccgctt ggtcgcgctg caagcgaatg
      901 ccgctgccga ggaaatattc cgggtcgatt ctgccggcga tcaaatttgc atcggtcacc
      961 gtcggctcga ccccgccttt gccatagcag gccggaccgg gcgccgcgcc ggcgctctgc
     1021 ggcccgactt tcaaaccgcc ggcgggatcg atccaagcga tgctgccgcc gccgttgccg
     1081 acctcgacga tgtcaactac cggcaacatc atcggatggc cggtgacgta accgccgacg
     1141 tagtagctgc tcgtcacttc gggatgaaag tctttgatca agctcgactt tgccgtggtg
     1201 ccgcccatgt cgaaggaaat gatatggcgg cagccgagcg cttcaccaag ccgcgcggcg
     1261 gcgataacac ccgccaccgg ccccgattcc atcatggtca ccggcatttt cttggcggtc
     1321 tcagccgaca tgacgccgcc gttggactgc atgacgcgaa aggtgccgcg aaaaccggcg
     1381 gcgctgagca tcttttccag cgagagcaaa tagcggcgca ccagcggccc gacgtaggaa
     1441 ttgagcgccg tcgtcgaagt gcgctcgtat tcgcggaact cgcgtagaat ctcgtgggac
     1501 agcgacacgt aggcttcggg aaactgttgg cgcagcaact cgccgagccg ctgctcgtgc
     1561 gccgcgttgg cataggaatg gagcaaacac accgcgacgc tttcgacgcc ggcttttttt
     1621 aacgcagcta tcgccgccgc cgctgaagtt tcatcaagcg gggtgagcac ttcgccggtc
     1681 gcatagagtc gttcgtccac ttccaggcgc aagtcgcgcg gcaccagcgg caccggccgt
     1741 ttgaaaaaca aattataacc ttccggacgg ttgccccggc cgatctcgta gacgtcgcga
     1801 aaacccttgg tggtgattag accggtacgc gcgcctttgc gctccagcac cgcgttgatc
     1861 gttaccgtgg aaccatggac aaagaagctg gcgtcgccaa cttcaacatt ggcacctttc
     1921 aaacaatcgt cgatgccgcg cgctaaatcg tccggtgtcg tcagcgcttt actgcgcgac
     1981 acccggccgt cgtcatcgac ggcaaccaaa tcggtaaacg tcccaccgat atcaactgcg
     2041 attctcatgg agtctttcag tcaaaataaa tcaatccaaa tttaacgatt tcacggggcg
     2101 agcttcctcg cttagcctgg tatgataatt tttcccctgt cacgacgaac ggagcaaggg
     2161 gtctcgtctt ttatcgggct catgccacca acgacttcgc gccgcttgcg gttacatgaa
     2221 aactatcgcc gtagagcacg atcttccctt cgctctcgat gatcacacgc agcgcgaccg
     2281 tcatattctc attcaaagtc gatgcaccga cggtggttgc accaacttca tgggcgtcac
     2341 ttgcattcaa aaacggcgct tcccattggt tgaggccgat gccattggcg aggccgtaaa
     2401 cggctgcctt cgagtagaac tcgcccagtt cattgcgggc ggtttcgtcg atcgcggcga
     2461 cggcgccgtt gggccggagt tgccgcgcca tcgccgcaac gattttccgc gcgcggtcat
     2521 agcaggatag aatcttgtta tcgcctgaca atataaaggt ccggccggcc tcggcccagt
     2581 agcgctcatg ctggacggcg agataaaccg cccaatggga accgacgctt gccgcctgtt
     2641 tgaagctcgg cggattgagc cgctgggcgc cggcgaggat gcgaatatct tcggctccca
     2701 tatcgcgcgc caagcgatcg atatcggcga cgacctcata ctccttgcgc cccggcttga
     2761 tgaaggcaac ggcaccggcg cagacttctt ttagcagccg gccggcttca gacatggcgc
     2821 cgagctcacg cgcagtcttg gccaaccgca acggcgcaac gctgtcatcg caatccagcc
     2881 agctcacgtc gggcagcgcg gatttcatat cctccaactg cggcagcggg aatccatgac
     2941 cggaatccgc taaaccgatc ctggcgctct cgaagccttt ctctttgatc atcttggcgc
     3001 catcgctacc gacctgattc gacgcgcgca catcttcgac ccaggtgagc gtcttggcga
     3061 acggcacatc gcgactgccg atattgagca acatggatat cgccccttgc cgcggcacca
     3121 cagcgatgcc gccgcgcact ttggggaaat aattggtcag gtaacaaaga tcggcgaagg
     3181 aatagttgtc gccgtaaatc accaaagcgt cgagatcgcg gcgcgccatc tcccggcgca
     3241 caccatcgag gcgcgcttga aactccgcct gcggcaccgc cgccgcatcc caagtagaac
     3301 aaccacgctt caagactgaa tgtcggccaa aaatcatatt gtcctcatcg actccgactc
     3361 gatccgacga tttgaacgac ttgaacgttt tgaacgattg gaacgagaag gttcaagccg
     3421 ttcaaatagt tcaattcgtt caaaacgttt aatttcatcg gaggctactc tttaatatgg
     3481 agatcccacg acgattgcat caacgactcc gcctgaatct ttcccatcac cacggtatcg
     3541 ccaagcgtaa gataaccggt ttccggcaca tattggttgg gatgaatgat gaaagtcatc
     3601 ccctcttcca actgggtctt gttgtcatct tctaggctgc ccggcgccga cgaaccgcag
     3661 cccaggccat ggccacgcac gcgcatgaac ggcggccggc aatactctgc gtagccatat
     3721 tcaataaacg tcccgttgat cgccttggca acggcgttcg ccggcgcgcc ggtctgcgcc
     3781 gcggccatgc ccaagcccat cgctttgtgc aggatcgcgt acttctcgcg caccaccggc
     3841 ggcggttcgc ccaacaccac ggtgcggcag atctgcacga acagtccgcc gatgcacggc
     3901 gtgatctcgc cgataataat atctcccggc tgaacgatcc gatcagtggg tggatgaagc
     3961 gcttgattgt gattgttcgc caccaccatg ccgaaattat cttcggcgcc gagggaacgc
     4021 atcttatact cgacgatggc ggcgagctcg tattctttca atcccggctg cgcgtcttcg
     4081 tgcaaggcgc tcaccccggc atcggcgatc gccgccgcgc gccgcatacg ctccagctcg
     4141 aacggaccct tgcagcgcgc catcgtctcg atgatgtctt ttgcgttcac cggcgtccga
     4201 cccaggactt tgacgatcgc gtcatgcagc ccgacatgca tcgcttcccg gccgatcaat
     4261 cccacattgc caaccttgcc ggccatcttc gccaagcggt cgtggaaatc caggaatggc
     4321 cggacatcgg cgaccca
//
LOCUS       DASQKS010000438         6495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_664816, whole genome shotgun sequence.
ACCESSION   DASQKS010000438 DASQKS010000000
VERSION     DASQKS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664816"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VE170_11275"
     CDS             complement(<1..207)
                     /locus_tag="VE170_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HYJ16061.1"
                     /translation="METKNLFANLANAQRGEEFLTLFDNANVKIERIVSHANPSPAGF
                     WYDQDEDEWVVVLRGSATLEFAGGE"
     gene            complement(402..1064)
                     /locus_tag="VE170_11280"
     CDS             complement(402..1064)
                     /locus_tag="VE170_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYJ16062.1"
                     /translation="MTTPLDILVTGGTGYIGNHLIPLLIARGHRVRVLAREQSLSRVP
                     SGAVGVKGDALDADSVAGALRPGDTVIHLVGTPHPSPSKADQFEKVDLISIRSTVSAA
                     QKVGIAHLVYISVAQPAPIMQAYLWVRMLGETMIREAGLTASIVRPWYVLGPGHWWPK
                     LIQPLYKLADMIPSLRPAVERLGLLNIEQMVAAMAFAVENPPAQGQRRIYDVPAIRRA
                     KL"
     gene            complement(1091..1828)
                     /locus_tag="VE170_11285"
     CDS             complement(1091..1828)
                     /locus_tag="VE170_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYJ16063.1"
                     /translation="MTLPGWPNSCDGLRVAVLADLHVGSPFNGVDKLEQIVDLTLKAK
                     PELILLAGDYTIHGVVGGNVVAPEIIAKGLSRLTAPLGVYAVLGNHDHREGAKRVRRA
                     LESNGIKVLEDDAIPLKLAECQIWLAGLSDLWTGRRNYQAALALIPQGAPAIAFTHNP
                     DSFPVLPKPVTLTIAGHTHGGQVRLPLLGRLVVPSEYGERYAIGHVVENGRHLFVSPG
                     LGTSIFPVRFLVPPEVSMLTLRSEKHK"
     gene            complement(1952..2974)
                     /locus_tag="VE170_11290"
     CDS             complement(1952..2974)
                     /locus_tag="VE170_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HYJ16064.1"
                     /translation="MKRIVICFDGTWNRPAEESIPETQQVETNVRRFYESVRPQSADG
                     VKQVKWYDEGVGTKWYDRAIGGGIGAGLEINIVQGYEFLAKEYQEGDEIYVLGFSRGA
                     YTARSLVGMVRNCGLIYPKHLGLRALMAYGIYRTRDDNVDSATAKLFRSSFCRDVAIK
                     FIGVWDTVGALGVPLDILKDVHMKFYEFHDTNLSNIVENGYQAMAVDEHRNDYDVCTW
                     SPAEKLQQNIEQRWFIGAHCDVGGGYPDRRLSDLPLRWMQDKASALGLALDPVVIDGK
                     NHLGASTDSYAKFLKGLYAKRNARHYRAIGATKFGNEVVDPSVQQRRKDDRDYEPQNN
                     GLPKLV"
     gene            complement(3063..4763)
                     /locus_tag="VE170_11295"
     CDS             complement(3063..4763)
                     /locus_tag="VE170_11295"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ16065.1"
                     /translation="MADAIADVCIVGVGGMGGIIAKELASAGLKVVGFERGPAPKKAD
                     YAPRDSIRFLIRPEQLEWVRHEPTTTRKKAGQKTNLQYRTSPLNVLGGALLHWTGQSS
                     RYMPDDFKIYSNEIASGSAERAKADLTGYDIIDWPLSYDDLEPYYEKFEWEFGVSGQA
                     GLNPFAGPRRRGFPLPPLRHSGKMQLFAEACRKLGYHPYDTAAGIASQPYKPAAPFDT
                     RIDARPACVYCGHCNFYGCHVHAKSASLYITIPVAVQTGNFELKTNSKVCRINSDASG
                     QVTGVNYFDADGREHEQRARVVILSAFIFEHARLLLLSKTDSGKFAKGLANSSGYVGR
                     NILAHGDVRAMGAFDDFVINGYIGPGSAAMRIDDFNGNNFDHTGLGFIRGGTIGTSGD
                     GTPVTRVDVLPPDVPTWGKEFKEFYQRYYNRIMDLNMQPETLPHKANRIDLDPRARDR
                     WGLPLPRVTFDFHQNEHRLQKYLAGVGDRIMRATGASKVWTEEKGRPNRWAGGTRMGA
                     DPKNSVVNQYCQTHDIANLFVVGASVFPTMAGYPPTATVAALSYRTSEFILQQKDWFK
                     "
     gene            complement(4765..5268)
                     /locus_tag="VE170_11300"
     CDS             complement(4765..5268)
                     /locus_tag="VE170_11300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HYJ16066.1"
                     /translation="MDSNPRILTNLERRTLEAIAERIFPKTDSPGAVEIGAVDYIEIA
                     LAGDYAALLPLYRIGMKALNRHAKTACGGQFARLDGKQQDELLKAFESGAVVGFKKAA
                     EFFDMVKYHVFEGLFCEPHYGGNKNMDGWRAVGFPGQQWGYPDPYINKPVDMAPVAVD
                     YTKAEMK"
     gene            complement(5234..5617)
                     /locus_tag="VE170_11305"
     CDS             complement(5234..5617)
                     /locus_tag="VE170_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16067.1"
                     /translation="MSSRWWKIAHSKLKLAAIDPQIAEDLNRHASESARFIFANVVSL
                     RRVPADSAIERAWRFKTAGSNLCHFIPVSIVFARCDEHRPSSFRDLPRICFFFTGDFC
                     YSNSILVMKERLSRHGLKSKNSNQS"
     gene            complement(5802..6395)
                     /locus_tag="VE170_11310"
     CDS             complement(5802..6395)
                     /locus_tag="VE170_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYJ16068.1"
                     /translation="MSNLTGRICVFTGSRHGARSEYAEAAKRLGRELVARGYGLVYGG
                     GKVGLMTVIADTVLELKGHVTGVIPSSLVSKEVAHLGLTALRVVDSMHERKALMAELS
                     DGFIAMPGGIGTMEELFEVFSWAQLGLHEKPCALLNVAAYYDPLIQFLDRAVEQDFIK
                     TKHRALLIVESDPGVLLDRFAELIAVKPVEKFDAKVT"
BASE COUNT         1401 a         1978 c         1756 g         1360 t
ORIGIN      
        1 ctcgccgccg gcgaattcta aagtcgccga gccccgtaac accacgaccc actcgtcttc
       61 gtcctgatcg taccagaaac ccgccgggct cggattggca tgagaaacga tgcgttcgat
      121 cttgacgttg gcattgtcga atagcgtcag aaattcttca ccgcgctgcg cgttcgcgag
      181 attggcgaat agattctttg tttccacggc aaagatcctg atcaaggaat tagtttagga
      241 tgatgatcgg aagatcccgt tcctgtcatc ccgagcacag cgagggatct cgactggtcc
      301 gacccttgcg cggacgagat tccgcggcgt cgcctcggaa tgacaaatac gatctagtct
      361 cttgagaaca aaccaatcct tagacaggaa attcaaaact ttcacaactt cgcccggcga
      421 atcgccggca cgtcgtaaat gcgccgctgc ccctgtgccg gcggattttc cacggcaaac
      481 gccatcgccg caaccatctg ttcgatgttc agcaacccca agcgctccac cgccggccgc
      541 agcgacggta tcatgtcggc aagcttgtac agcggctgga ttaactttgg ccaccagtgg
      601 ccgggaccca gtacgtacca tggccggacg atgctcgccg tcaggccggc ttcgcgaatc
      661 atcgtttctc ccaacatgcg cacccagaga tacgcctgca tgatcggcgc cggctgggcg
      721 acactgatat aaaccaaatg agcgataccg accttttgcg ctgcgctgac cgtcgaacgg
      781 attgatatca gatcgacctt ctcgaactga tccgctttac ttggactcgg atggggcgtg
      841 ccgacgagat gaatcacggt atcgcccgga cgcagtgcgc cagccacgga atcggcgtcc
      901 agcgcatcgc ccttcactcc caccgcgccg ctcggcacgc gactcagtga ttgttcgcgc
      961 gccaacacgc gcacccgatg gccgcgcgcg atcagcagcg ggatcaaatg attgccgatg
     1021 tagccggtgc cgccggtgac aagaatatcg agtggcgtgg tcattgggcc gattctaatt
     1081 tgctggacac ttacttatgt ttctcggagc gcagggtcaa catcgacact tccggcggga
     1141 ctaaaaaccg cacggggaaa atgctggtgc cgagacccgg actgacgaac aggtgccggc
     1201 cgttctccac cacgtggccg atggcataac gctcgccata ttccgacggc acaacaagtc
     1261 gccccaacaa cggcaatcga acctggccgc catgggtatg tccggcaatg gtcagagtga
     1321 ctggtttcgg cagtaccgga aagctgtccg gattatgcgt gaatgctata gcaggcgcgc
     1381 cctgtgggat gagcgccaat gccgcttgat agttccgcct gccggtccat aaatcgctca
     1441 agcccgccag ccaaatctgg cattctgcca atttcaacgg gatcgcatcg tcttcgagaa
     1501 ccttgatgcc attggactcc agcgccctgc gaactcgctt ggccccttcc cgatgatcgt
     1561 gattaccaag cacggcgtat acgcccaacg gtgcggtcaa gcggctcaac cccttggcaa
     1621 ttatttccgg cgcaacgaca ttgccgccga ccactccatg gatcgtgtaa tcgccggcga
     1681 gcaaaatcaa ctcgggtttg gccttgagcg ttagatcgac aatttgctcc agcttatcga
     1741 cgccgttgaa cggcgagccg acatgcagat ccgccaagac tgcgacacgc aagccatcgc
     1801 agctgtttgg ccatcctggc agtgtcattt catggtcttc attactcaga ctcgccggct
     1861 cgagccaaaa tgcccacaag cccaagccaa gtgcgactaa cgaagcgcaa acaaaaagcc
     1921 gccgtagccc aatccccatc cacagactat atcacaccag cttcggcagc ccattatttt
     1981 gcggctcgta gtcgcggtcg tctttgcggc gctgctgcac cgacgggtcg accacttcat
     2041 tgccgaactt ggttgcgccg atggcgcggt agtgccgggc attgcgcttg gcgtagaggc
     2101 ctttgagaaa cttggcatag gagtcggtgg atgcaccgag atgatttttg ccgtcgatga
     2161 caacagggtc gagcgccaag cccagcgcgc tggccttgtc ctgcatccag cgcagcggca
     2221 aatcggacaa gcgccggtcg gggtaacccc cgccgacatc acagtgcgcc ccgatgaacc
     2281 aacgttgttc gatattctgc tgcagcttct ccgccgggct ccatgtgcag acgtcatagt
     2341 cgtttcgatg ctcatccacc gccatcgcct gatagccatt ttcgacgatg ttgctgagat
     2401 tggtatcgtg gaattcgtaa aacttcatgt gcacatcttt gagaatgtcg aggggcacgc
     2461 cgagggcccc gacggtatcc caaaccccga taaatttgat cgccacgtcg cggcaaaagg
     2521 acgagcgaaa cagcttcgcc gtcgcggaat ctacgttgtc gtcgcgggtg cgatagatcc
     2581 cgtaagccat caaggcgcgg agcccgagat gttttgggta gatcagaccg cagttgcgca
     2641 ccatgccgac taaactgcgc gctgtgtacg cgccgcggct aaaaccgagc acataaatct
     2701 cgtcgccttc ctggtattcc ttggccaaga attcgtagcc ctgaacgata tttatttcca
     2761 acccggcgcc gataccgccg ccgatcgccc ggtcatacca cttggtgccg actccttcgt
     2821 cataccattt gacttgtttg accccatccg cagactgggg acgcaccgat tcgtagaaac
     2881 gccgcacatt ggtctccacc tgttgagttt ccggtatcga ttcctccgcc ggcctgttcc
     2941 acgtcccatc gaagcaaatg acaatgcgct tcataaaacc tcccggtaag actggactga
     3001 tggagtactg gagtgttggg ttcataaacc caacgctgca ccacttgatt tttttcccta
     3061 tcctacttga accaatcttt ctgctgcaag ataaactccg aagtccgata actcaacgcc
     3121 gccaccgttg cagtcggcgg ataaccggcc atggttggaa acaccgaggc gccgacgacg
     3181 aagagattgg caatatcgtg agtctggcag tattgattga cgacggaatt tttcgggtcg
     3241 gcgcccatgc gcgtgccgcc ggcccagcgg ttgggccggc ctttttcttc cgtccaaact
     3301 ttgctcgcgc cggtggcgcg catgatgcga tcgcccaccc cggccagata tttctgcagc
     3361 cgatgctcgt tctgatgaaa atcgaaggtc acccgcggca gcggcaaccc ccagcggtcg
     3421 cgcgcccgcg gatcgagatc gatgcgattg gctttatgcg gcaaggtctc cggttgcatg
     3481 ttcaggtcca tgatccggtt gtaatagcgt tggtaaaatt ccttgaactc cttgccccag
     3541 gtcggcacgt ctggcggcaa tacgtcgacc cgcgtcaccg gcgtgccgtc gccgctggtg
     3601 ccgatggtgc cgccgcgaat aaaacccagg cctgtgtgat cgaagttatt gccgttgaaa
     3661 tcgtcgatgc gcatggccgc cgagcccgga ccgatgtagc cattgataac aaaatcgtcg
     3721 aaggcgccca tggcgcggac atcgccgtgg gcgagaatat tgcgcccgac atagccgctg
     3781 gaattagcca aacctttggc gaattttccc gaatcggtct tcgacagcaa cagcaagcgc
     3841 gcatgctcga agataaacgc gctcaatatc accacccgcg cccgctgttc gtgctcgcgg
     3901 ccatcggcgt cgaaatagtt cacgccggtc acttggccgc tggcgtcgct gttgatgcgg
     3961 cagacttttg agttggtctt gagctcgaag ttaccggtct gcactgccac cgggatcgtg
     4021 atgtagagcg acgccgactt ggcgtgcaca tggcaaccgt agaaattgca gtggccgcag
     4081 tacacgcagg ccggccgcgc atcgatgcgc gtgtcgaagg gcgccgccgg cttgtagggc
     4141 tgggacgcga tgccggcggc cgtgtcgtag ggatgatagc cgagttttct gcacgcctcg
     4201 gcgaacagct gcatcttgcc gctatggcgc agcggcggca gcggaaaacc gcgccggcgc
     4261 ggcccggcga aaggattgag cccggcctgc cccgagacgc cgaactccca ttcgaacttt
     4321 tcgtaatacg gttcgagatc gtcgtagctg agcggccagt cgatgatatc gtagccggtg
     4381 aggtccgctt tggcgcgctc ggcgctgccg ctggcgattt cattgctgta aatcttgaag
     4441 tcgtcgggca tatagcgcga cgactgtccg gtccaatgca gcaaagcacc gcccaacacg
     4501 ttcaagggac tcgtgcgata ttgcaagttg gttttttgac cggccttttt ccgcgtcgtg
     4561 gtcggctcat ggcgcaccca ttcgagttgt tccggacgga taagaaaacg gatcgagtcg
     4621 cgcggcgcgt agtctgcttt ttttggtgcc gggccgcgct cgaagccgac cactttcaaa
     4681 ccggcggagg ccaactcctt ggcgatgatg ccgcccatgc cgccgacgcc gacaatacaa
     4741 acatccgcga tggcatccgc catgctattt catctccgcc ttggtgtaat ccaccgccac
     4801 cggcgccatg tctaccggct tattgatata ggggtccggg taaccccact gttggccggg
     4861 aaatcccacc gcccgccaac cgtccatgtt tttgttgccg ccataatgcg gctcgcagaa
     4921 caatccttcg aagacatggt atttgaccat gtcgaaaaac tccgcggcct ttttgaatcc
     4981 caccaccgca ccggactcga aagctttgag aagctcatcc tgctgttttc cgtccaggcg
     5041 ggcgaactgg ccaccgcacg ccgtcttggc gtggcgattg agcgctttca taccgatgcg
     5101 ataaagcggc aacagcgccg catagtcacc ggccaaggcg atctcgatat aatccaccgc
     5161 gccgatctcc accgcgcctg gactgtcggt cttgggaaaa attcgctcgg cgatcgcttc
     5221 gagagttcgg cgctcaagat tggttagaat tcttggattt gagtccatgc ctgctaagcc
     5281 gctccttcat caccagtatc gaatttgaat agcagaaatc accggtgaaa aaaaagcaga
     5341 ttcgcggcaa atcccgaaaa gatgatgggc ggtgttcgtc acatcgagca aacacgatgg
     5401 atacaggaat gaaatgacac aagttcgaac ctgctgtctt gaagcgccat gcccgctcta
     5461 ttgcagaatc cgccggtacg cggcgtaaac tcacgacatt agcgaaaata aatctcgcgg
     5521 actcgctggc gtggcgattc aaatcctcgg cgatctgcgg atcgatggcg gccagtttca
     5581 gtttgctgtg agctattttc caccagcggc tcgacatcgg cgcgtcccga aatgcaatta
     5641 gacgtgcggc aaaactaccg ggaatctttc gacagtaagg aatcgagcag cagacgcgcc
     5701 gcgttgggat gggggctctt tggctgccaa tgccacgggc gcaagagata cgaactcggg
     5761 attcaccgtg ttgatccatt cgatcggcgt gatctctttc gctacgtcac ttttgcatcg
     5821 aatttttcca ccggcttaac cgctatcaat tcggcgaagc gatcgagcaa cacgcccggg
     5881 tcgctttcga caatcagcaa cgcgcgatgc ttcgtcttga taaaatcctg ctcaacagca
     5941 cgatcgagaa actgaatcag cggatcgtaa tatgccgcga cattcaacaa agcgcagggc
     6001 ttttcatgca accccaactg cgcccaacta aaaacctcga acaactcttc catggtgccg
     6061 ataccgcccg gcatggcgat aaaaccgtcg gacaactccg ccatcagcgc cttgcgctcg
     6121 tgcatcgaat cgacgactcg caaagcggtc aagcccaagt gcgcaacttc tttgctcacc
     6181 agagaactgg gaatcacacc ggtgacatgt cctttcaatt cgagaaccgt gtcggcgatc
     6241 accgtcatca aaccgacctt gccgccgccg taaacaaggc catagccacg cgccaccagt
     6301 tcgcggccaa ggcgtttcgc cgcctcggcg tattcactgc gcgcgccatg gcgcgaaccc
     6361 gtgaacacac agattcgccc ggtcaaatta ctcatcgaag tgaagctctc cgcaactttt
     6421 tatcatcgcc ctgcagcgcc gattctaaat ggaaacagcg cattcgtcta tggcctgcga
     6481 gcggccaaag aactc
//
LOCUS       DASQKS010000439         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1910806, whole genome shotgun sequence.
ACCESSION   DASQKS010000439 DASQKS010000000
VERSION     DASQKS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910806"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VE170_11315"
     CDS             complement(<1..277)
                     /locus_tag="VE170_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16069.1"
                     /translation="MRVALSTPTPHLAPLFVAKDKRFAEKYGLDVQLIVVNSGSLVAQ
                     MFQAREIQITANALGSLVSLAVTDEKMSFFLDLSNTSPFSVVIQPAIR"
     gene            complement(425..>2789)
                     /locus_tag="VE170_11320"
     CDS             complement(425..>2789)
                     /locus_tag="VE170_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ16070.1"
                     /translation="QEGQEKARTVLEFAAGLKKTATRTGLMTFGAAPEWEFPPRDGAL
                     PVEFSTHQDREQSDIQAALQAAAAQIGEGRQGRILLFSDGNENRGESARVVPLLRSQG
                     VQVWTLPVGLSRGRNEIYLADLVLPRQVDSAESFEIRGAIESLREAPARLRLLRDGVL
                     SAERELRLSAGSNNVSFRDTLNERGNHRYELLVESADDTLAENNLLQGVVEVKGPPRV
                     LLLSAQKDSQRVLSRVLQVQGYAVVEAAPEAHSVSLAELAAFDLLVLDNVPAFQLTHA
                     KMESIEKYVRDFGGGLLVVGGSQSYGAGGYYRTPLERILPVDMRPPAKLEMPHVALLF
                     VIDKSGSMGSGGEGSTKLDLAKAAAIAAADIMNPTDQVGILAFDANWDWALPFRQVGK
                     GEWITEKLANLQSDGGTDLYKAMVEAYRGIAGKTASIKHVIALSDGLTDKADFQALVA
                     KMARDGITVSTVSIGSDADVKLMAEIAKEGKGRGYVALDPQTIPQIFTTETLLISRDL
                     LIEKAVAPSIVAATGALKGIPQNNLPALRGYVLTYPKPRAELLMRVDTDPLLVSWRYG
                     LGRVMAFTSDLSGRWGKDWVGWNAFPQWASQIGRDTMRKILDGKLRVDFHSDGDAVKV
                     VGDLVAQDGKFLNHLKIQATVVAPNQTSREQPLQQSAPGRYEGKFTPAQRGIHFITLY
                     AEGQAGAAPLPVATVPYIAPYPKEYRDLKPNLALLSRLAEETGGEMLDAEKFHDGLKR
                     LYTPTPGKSSQGQETWWPLASAGLLLFLTDLVLRSWPWRRTAILNPS"
BASE COUNT          541 a          869 c          803 g          576 t
ORIGIN      
        1 gccgaatagc cggctggatg accacggaaa atggcgaagt gttgctcaag tccaaaaaga
       61 aactcatctt ctcatcggtg acggcgagac tgaccagcga ccccaacgca ttggcggtga
      121 tctgaatttc cctggcttga aacatttgcg ctaccagtga tccgctgttg accacaatca
      181 attgcacgtc gagcccgtac ttttcggcga atcgcttgtc cttggcgacg aacaacggcg
      241 ccaggtgcgg cgtcggcgtc gacagcgcca cacgcacctg caggagcttc tacgcgtcta
      301 cggatgaatt gacgactaga cttgtgagca aagataccaa aatagtgact attgggttca
      361 agtgaatcct ccatttgtcg tcgatctttg aagttcgggc accgcccatt caagcaatca
      421 gctattatga tgggtttaaa atagcggttc gccgccatgg ccagctgcgc agcaccaggt
      481 cggtaagaaa cagcagaagt ccggcgcttg ccaacggcca ccaggtctcc tgcccctgag
      541 aacttttccc cggcgtcggg gtgtaaagcc gcttgaggcc atcgtgaaac ttttccgcgt
      601 cgagcatctc accgccggtt tcctcagcga ggcggctgag cagcgccaag ttgggtttca
      661 ggtcgcgata ttctttaggg taaggcgcga tgtatggcac cgtggccacc ggtaacggcg
      721 ccgctcccgc ttggccctcg gcgtagagcg taatgaaatg gatgccccgc tgcgccgggg
      781 taaactttcc ttcatagcgt cctggggcgc tttgctgtag cggctgctcc cgactggttt
      841 gattgggcgc aacgacggtc gcctggattt ttaaatgatt caaaaacttg ccgtcttggg
      901 cgactaaatc gccgaccact tttacggcgt cgccgtcgct gtgaaaatcc acgcgcagct
      961 tgccgtcgag aattttccgc atggtgtcgc ggccgatctg gctagcccac tggggaaaag
     1021 cgttccagcc gacccagtct ttgccccacc gcccgcttaa atctgaagtg aatgccatca
     1081 cccggccgag accgtaacgc caggacacca gcaacgggtc cgtgtcgacg cgcatgagca
     1141 attcggcgcg cggcttggga taggtgagca cgtagccgcg cagtgccggc aaattatttt
     1201 gcgggatacc tttgagcgct ccggtggcgg cgacgatact cggcgccacc gctttctcaa
     1261 tcagcaaatc gcgcgagatc aacaatgtct cggtggtaaa aatttgcggg atcgtctgcg
     1321 gatcgagggc gacgtagccc cgccccttgc cctctttggc gatctccgcc atcagcttca
     1381 cgtcggcgtc gctgccgatg gacaccgtcg aaacggtgat accgtcacgc gccatcttgg
     1441 ccaccagcgc ctgaaagtcg gccttgtccg tgaggccgtc ggagagcgcg atcacatgtt
     1501 tgatcgatgc cgtcttgccg gcgatgccgc gataggcttc gaccatggct ttatacaaat
     1561 ctgtcccgcc gtcggattgt agattggcca gcttttcggt gatccattcg cctttgccca
     1621 cctgccgaaa cggcagcgcc cagtcccagt tggcgtcgaa ggcgaggatg cccacctgat
     1681 ccgtcgggtt catgatatcg gcagcggcga tagcggcggc cttggcgaga tcgagcttgg
     1741 tgctgccttc gccacccgag cccatgctgc cggatttgtc gatgacaaac agcagcgcga
     1801 catgaggcat ttccaatttc gccggcggtc gcatgtcgac cggcaagatg cgctccagcg
     1861 gcgtgcggta gtatccgccg gcgccatagc tttgcgagcc accgacgacc agtaatccgc
     1921 cgccaaagtc gcgcacgtat ttttcaatac tctccatttt cgcgtgggtg agctgaaatg
     1981 ccggcacgtt gtcaaggacc aataggtcga aagcagcaag ctccgcaagc gaaacggaat
     2041 gagcctcggg cgccgcctcc accacggcgt agccttgtac ttgcagaact cgggaaagaa
     2101 ctcgctggct gtctttctgc gccgacagca acagcacccg tggcggtcct ttcacttcga
     2161 cgacgccttg gagcaggtta ttttccgcca gcgtgtcgtc cgccgattcg accaagagct
     2221 cataacgatg attgccgcgc tcgttgagcg tatcgcggaa ggaaacgttg ttgctgccgg
     2281 cgctcaagcg cagttcccgc tcagccgaaa gcacgccgtc gcgcagcaag cgcagccgcg
     2341 ccggcgcctc gcgcagactc tcgatcgcgc cgcgaatttc gaagctctcg gcgctgtcga
     2401 cttggcgcgg caagaccaga tcggccagat aaatctcatt gcggccacgc gataggccca
     2461 ccggcaaggt ccatacctgc acgccttgac tacgcaagag cggcaccacc cgcgcgctct
     2521 cgccgcggtt ctcgttgccg tcggagaaga gcaatattct cccctgccgc ccttcgccga
     2581 tttgcgccgc cgcagcttgg agggcggctt ggatatcgct ttgctcccgg tcctggtggg
     2641 tcgaaaattc caccggcaga gccccgtcgc gcggcggaaa ctcccactcc ggtgcggcgc
     2701 caaaggtcat cagcccggtg cgcgtcgcgg tttttttcag acccgcggca aactccagaa
     2761 ccgtgcgcgc cttctcctgt ccctcttga
//
LOCUS       DASQKS010000440         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5693701, whole genome shotgun sequence.
ACCESSION   DASQKS010000440 DASQKS010000000
VERSION     DASQKS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5693701"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VE170_11325"
     CDS             <1..828
                     /locus_tag="VE170_11325"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16071.1"
                     /translation="EMGFSKKYGVDLETILIQAGSPNIQALVGGSLQLTQTAASSAVI
                     GANQGAPVVIVATLENKLPMLLVSRPEIKEPQQLRGKVIGINRFGGSNDAAVLMAIKA
                     WKMDPKDISMLQTGGTSARMAALLTKKVDATVQSYPEIYQARKLGMNVLADIGDFGFF
                     TNTSNIVTRAELQQNRGAIKGFLKGQIEGMHYVKTNKEGSLKILRKYLQIADAEAVDG
                     TYEFFAKRLARTPRTELEGIKNILLSINAGQRNPTEFVDMSLLDEIEKEGFVQKLYQ"
     gene            950..1939
                     /locus_tag="VE170_11330"
     CDS             950..1939
                     /locus_tag="VE170_11330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16072.1"
                     /translation="MSAKLRLSVACGDYEIIRALKDGTVQADGLELVVLTSHGPRERH
                     WRMARNLEYDVCEFNVGAYFMAREHGVAVTSIPVFLHRRFRHGFVFINVNAGIESPKD
                     LIGKRVGGTNFQPAGNIWIRGILAEHYGVPHESITWLVDRSEDVEFTPPPGLKIEMIP
                     TGKHMDTMLAEGEIPAMINPYIPRPIVDGDPRVSYLFPNYKEVEREYFQQTGIFPIMH
                     VTVIKQEIVDKHPWAAVSLAKAFEKAKQLCYQRVVNPRIVPLAWFAHQWQEERQVLGA
                     DPWAYGLGEANRKNLQTIIRYCHDQGLIKREMTVDELFFDTDPGDAGGDEGHY"
     gene            2082..3104
                     /locus_tag="VE170_11335"
     CDS             2082..3104
                     /locus_tag="VE170_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16073.1"
                     /translation="MNSLAFSSRVFLGMAACLALVFSGRTVGAAEGLQKLRVAYAAIT
                     AAFSLPWLAKEAGIFQRHGLDVELVYIASGARAVQTLVGGSVDVVEIGGPAGVDARLA
                     GADTVYVALPVTRVLVFTVAAPQIQRIEEMRGKTIGVTRIGTVTDFFTRLYLRQNNLV
                     PDKDVMIRQAGGLPEIVAALKAGQVEGGTFGFPAVLHARAAGFRVLVDYNVSGYRYPL
                     SSVIVTQRLLRNNESAVRRLLEAHIEAAHRFKTDASFAMNVIGKYTQTSERAMLEETQ
                     RVYAAAFERVPHPDLEAMKLGITQVSETNPKVRAVDPREFVDGRLLREIEASGFVKRL
                     YGETGK"
     gene            3250..4182
                     /locus_tag="VE170_11340"
     CDS             3250..4182
                     /locus_tag="VE170_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16074.1"
                     /translation="MIIDTHFHAFPGKFLELIPEAKNDVRGVGFHAFDHQEYLDVMDK
                     YGIDMGVLSNTGGRIEKGGDRAKALELCRVLNDGFAEAHAKYPRRFLAFARLPMLDME
                     DCVRELERCYKDLKMHGVMMPTNVAGKYIDEPEFKPFWDALAAGAKPLFLHPANAPCQ
                     ANWDKYSLHQKILWPTDSTLALSRIVYAGMFDRYPDLKLIASHLGAMILCYLDRLNWR
                     EGDPLCKEEPEVYFKKIFYDTAGPIRAPFIKLVCDTVGADQVLFGADYPHGREGRDDQ
                     FYPMTLQAMEELDVPAADKEKIYYLNAKKLFGLN"
     gene            complement(4317..5312)
                     /locus_tag="VE170_11345"
     CDS             complement(4317..5312)
                     /locus_tag="VE170_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16075.1"
                     /translation="MPHTAIDIHHHYVPQQLIEETKKHGKTLGVEVAGDKGSWSLSFS
                     GSKPHRLQPPIFDVEKHIEIMDQGQVRLATLEANTNSLGYRLNGEQGEGWCNLYNDCI
                     QLLAKARPDRFIGMAVVPLQDAARAARVLQHAIVDLKFRGAFIGTNVNGQYYNITAFD
                     PFWAKAQELDVMIVMHPEHIAGAERMTEYGLNAVCGNPADSTLSLGYMFYSGLFDRFP
                     NLKICALHGGGFLPFHLGRFDKEFVTGQKTRPALAKQPPSAYLKNLYFDTLVYDVDTL
                     EYLKAKVGTDKLMLGTDYPYTLGDWMAVEKVQALKCSDADKEAILTGNAKRLLKL"
     gene            complement(5340..>5558)
                     /locus_tag="VE170_11350"
     CDS             complement(5340..>5558)
                     /locus_tag="VE170_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16076.1"
                     /translation="KYLRITDPKVLEASYNSEIAAMEHRLEIHEAALQASLDEIAPLD
                     ARAKSIKPADMIDRRYLVELEKSGLFAK"
BASE COUNT         1188 a         1496 c         1666 g         1208 t
ORIGIN      
        1 gagatggggt tcagtaaaaa gtacggcgtc gatctggaaa cgatcttgat ccaagccggt
       61 tcgcccaaca tccaggcgct ggtcggcggt agcttgcaac tgacgcagac ggcggcgtct
      121 tcggcggtga ttggcgcgaa ccagggggcg ccggtggtga tcgttgcgac tttggaaaat
      181 aagttgccga tgctgttggt ctcgcgcccc gagatcaagg agccgcaaca gttgcgcggc
      241 aaggtgattg gcatcaatcg cttcggcggc tcgaacgacg cggcggtgct gatggcgatt
      301 aaagcctgga aaatggatcc caaggatatt tccatgttgc agaccggcgg cacctccgcg
      361 cgcatggcgg cgctgctcac caagaaggtc gatgcgacgg tgcagtccta tccggagatc
      421 tatcaggcgc gtaagctcgg catgaacgtg ctcgccgaca tcggcgactt cggttttttc
      481 accaacacct cgaatatcgt cacgcgcgcc gagttgcagc aaaaccgtgg ggcgatcaag
      541 ggatttctca aaggtcagat cgagggcatg cactatgtca agaccaacaa agagggcagc
      601 ctgaaaattc tccgtaaata tctgcaaatc gctgatgccg aagccgtcga tggcacttac
      661 gaatttttcg ccaaacgttt ggcgcgaacg ccgcgcacgg aactggaggg gatcaagaat
      721 attctcctct cgatcaacgc cggccaaaga aatccaaccg agtttgtcga catgagcttg
      781 ctcgatgaaa tagagaagga agggttcgtg cagaagctct accagtgaag aatggagtgt
      841 tggagaaatg gagtgatggg ttgaagatgt ccggatctta aaccaatact ccgtcacttc
      901 accactccat gacttactcc aattcgggtt taggatcggg ggagggttca tgtcagcgaa
      961 gcttcgattg tcggtggcct gcggcgatta cgagattatc cgcgcgctca aagacggcac
     1021 ggtgcaagcg gatgggctcg agctggtggt cttgaccagc catggtccgc gcgaacgcca
     1081 ctggcgcatg gcgcgcaatc ttgaatacga tgtctgcgag ttcaacgtcg gcgcctattt
     1141 catggcgcgc gagcatggcg tggcggtgac gagcattccg gtgttcctgc accggcgatt
     1201 ccgccatggt tttgtcttca tcaacgtcaa cgccggcatc gaatcaccca aggacttgat
     1261 cggtaagaga gtcggcggga ccaactttca gccggcggga aatatctgga ttcgcggcat
     1321 tttggccgaa cattacggcg tgccgcacga gtcgatcacc tggctggtcg atcgcagcga
     1381 ggatgtcgag tttacgccgc cacctgggtt gaagatcgaa atgatcccga cgggcaagca
     1441 tatggatacg atgctcgccg agggcgagat tccggcgatg atcaatcctt atattccccg
     1501 gccgatcgtc gatggtgatc cgcgggtttc ctatttattt cccaactata aagaagtcga
     1561 gcgcgagtat ttccagcaaa ccggcatctt cccgatcatg catgtcaccg tgatcaagca
     1621 agagattgtc gacaagcatc cctgggcggc ggttagttta gccaaggcct tcgaaaaggc
     1681 gaaacagctc tgctatcagc gggtcgtcaa tccgcgcatc gttcccttgg cctggttcgc
     1741 tcatcaatgg caggaggaac gccaagtcct gggcgccgat ccctgggctt acggcttggg
     1801 cgaagcgaac cgaaaaaatc ttcaaacgat catccgctat tgtcacgatc aggggctgat
     1861 caagcgtgag atgaccgtcg acgaattgtt tttcgacacc gatcccggcg acgccggcgg
     1921 cgatgaaggg cattactaaa tgtttgtcac caaggtcgga agaagagact aaccgcgaag
     1981 aacgcaaaag tataggcaca taccaggggc ggttttgaca tccgccctct tggttgggtc
     2041 gttgcgcttg ggcggaagct ataccggtga ggagaaaaaa catgaactcg ttggcttttt
     2101 cttcgcgcgt atttctcggc atggcggcgt gcctggcgtt ggtcttctcc ggtcgaaccg
     2161 tcggcgcggc tgaggggctg caaaaactgc gcgtagctta cgccgcgatc accgcggcct
     2221 tttctctgcc gtggttggct aaggaagcgg gcatcttcca gcgccatggc ttggacgtcg
     2281 aattggttta tatcgcgtca ggcgcgcgcg cggtccagac tttggtgggt ggtagtgtcg
     2341 acgttgtcga gatcggcggg ccggcggggg tcgatgccag gctggccggt gccgatacgg
     2401 tctatgtcgc tttgccggtc acgcgggtcc tggtcttcac ggtggcggca ccgcagattc
     2461 agcgcatcga agagatgcgc ggtaagacca tcggcgtgac gcgcatcggc acggtgacgg
     2521 actttttcac gcgcttgtat ctgcgccaga acaatctcgt gccggacaag gatgtcatga
     2581 tccgccaggc gggcgggctg ccggagatcg tcgcggccct taaggccgga caggtcgaag
     2641 gcggcacttt cggttttccc gcagtgttgc acgcccgggc ggcgggcttt cgtgtcttgg
     2701 tcgattacaa cgtctcggga tatcgctacc cattgtcgtc ggtaatcgtt acccaaaggc
     2761 ttttgcgcaa caatgaatct gcggtgcgcc ggttgctcga agcgcacatc gaagcggcgc
     2821 atcggttcaa gaccgacgcc agtttcgcca tgaacgtgat cggaaaatat acccagacca
     2881 gcgagcgggc gatgttggag gaaacccagc gggtttatgc cgccgccttc gagcgggttc
     2941 cccatcccga cttggaagcg atgaagctcg gtatcaccca ggtgagcgaa accaatccca
     3001 aagtgcgcgc cgtcgacccg cgcgagttcg tcgatggccg cttgctgcgt gaaatcgagg
     3061 cgagcggctt cgtcaagcga ctgtatggcg agacgggaaa gtaggcagcc gccgaaataa
     3121 gaatggccgc aaagaacgca aaggggcgca aaaaattccc aaaggtaggg gcgggtttga
     3181 aacccgccct caggtattct gatccaaaac catcgaacaa tcacggcatg accacgaaag
     3241 gaaactcaca tgatcatcga tactcacttt cacgcctttc ccggaaagtt tctcgagctg
     3301 atacccgagg ctaaaaatga cgttcggggc gtcggcttcc atgccttcga tcatcaggaa
     3361 tatctcgatg tcatggacaa gtatggaatc gatatggggg tattatcgaa caccggcggc
     3421 cggatcgaaa aaggcggcga tcgggccaag gcgttggagc tgtgcagggt actgaacgat
     3481 ggcttcgccg aagcccacgc caagtatccg cggcgctttt tagccttcgc gcgattgccg
     3541 atgctcgata tggaggattg tgtgcgcgag ttggagcgct gctacaagga tctcaagatg
     3601 catggcgtga tgatgccgac caatgtcgcc ggcaagtaca tcgacgaacc ggagttcaaa
     3661 ccgttttggg acgcgctggc cgccggcgct aaaccgttgt tcctccatcc cgccaacgca
     3721 ccgtgccagg ccaattggga caagtactcg ctgcaccaga aaatcctctg gcccaccgat
     3781 agcacgctgg cgttatcgcg tatcgtctat gccggtatgt tcgaccgcta tcccgacttg
     3841 aagctgatcg catcccattt aggcgcgatg attctgtgct atctcgaccg gctcaactgg
     3901 cgcgagggcg atccgctgtg caaggaagag ccggaagtct atttcaagaa aatcttttac
     3961 gacaccgccg ggccgatccg cgcgccgttt ataaaactgg tctgcgacac ggtgggcgca
     4021 gatcaggtcc tgtttggcgc cgactatccg cacgggcggg aaggacgcga cgatcagttc
     4081 tacccgatga ctttgcaagc gatggaagaa ttggatgtac cggcggcgga taaggagaag
     4141 atttattatt tgaatgcgaa aaaacttttc ggccttaact aaactagccg ttcaacagca
     4201 tttcgattcc ctctcccgct gggagagggt tagggtgagg gccgcagttt agtgagccct
     4261 catcccagcc ttctcccaga gggagaagga gcatgattag gcagctgcgt cgctccttac
     4321 aacttcaaca accgcttggc gttacctgtc aaaatcgcct ctttgtcggc gtcggagcat
     4381 ttcagcgctt ggactttttc taccgccatc cagtcgccca gggtgtaagg atagtcggtg
     4441 ccgagcatga gcttgtcggt gccgactttg gcctttaaat attccaacgt gtcgacgtcg
     4501 tagaccaggg tgtcgaagta gagatttttt aaataggcgc tgggcggttg tttggccagc
     4561 gccgggcggg ttttttggcc ggtgacgaat tccttgtcaa agcggccgag gtgaaacggc
     4621 agaaagccgc cgccgtgcag cgcgcagatt tttagattag gaaaacggtc gaacaggccg
     4681 ctgtagaaca tgtagccgag cgataatgtc gagtcggcgg gattgccgca gaccgcattc
     4741 aaaccgtatt cggtcatgcg ctcggcgccg gcgatatgct cggggtgcat gacgatcatg
     4801 acgtcgagct cctgagcctt ggcccagaaa ggatcgaagg cggtaatgtt gtaatactgg
     4861 ccattgacgt tggtgccgat gaaagcgccg cgaaatttta gatcgactat ggcgtgttgc
     4921 aatactcgcg ccgcccgggc ggcgtcttgc agcggcacca ccgccatgcc gatgaagcga
     4981 tccggccgcg ctttcgccaa caactgaatg caatcgttgt agaggttaca ccaaccttcc
     5041 ccttgctcgc cgttcaaccg atagcccagg ctgttagtgt tggcttccag cgtcgccaag
     5101 cgcacctggc cctggtccat gatctcgatg tgtttctcga cgtcgaagat cggcggctgc
     5161 aaacgatgcg gcttgctgcc actgaacgac agcgaccaac tgcctttgtc gccggcgacc
     5221 tccacgccca gcgtttttcc atgcttcttg gtttcctcga tcagctgctg cggcacgtaa
     5281 tgatgatgaa tgtcgatcgc tgtgtgtggc attgaaatct ttgcttcctc tcagatcgct
     5341 tatttggcaa acaacccgct cttttccaat tcgaccagat agcgccggtc gatcatgtcg
     5401 gccggtttga tcgatttggc gcgcgcgtcg aggggcgcga tctcgtcgag cgacgcttgc
     5461 agcgccgctt cgtggatctc cagccggtgc tccatggcgg cgatctccga attgtacgat
     5521 gcttcgagaa ccttcgggtc ggtgatgcgc aggtattt
//
LOCUS       DASQKS010000441        10213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2774004, whole genome shotgun sequence.
ACCESSION   DASQKS010000441 DASQKS010000000
VERSION     DASQKS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774004"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VE170_11355"
     CDS             <1..612
                     /locus_tag="VE170_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16077.1"
                     /translation="GLLAHLGISRCHAVGFALGGQIMQALAIERPDLVATLTIAASGA
                     GTKTLAGGQRNLSPHTEREIRAIGFERYIRNYIENDTMAFNRQFYRDHGNVVAALADA
                     VWSGQSTVEIFRRHEDARLTWDTLGKAAEVTVPTLVLCGADDDVARRGSTPVGTARRL
                     AELTPRAELCLIPNTKHMTFWDGNGALVALQEFLLRHPIAKSH"
     gene            1173..2240
                     /locus_tag="VE170_11360"
     CDS             1173..2240
                     /locus_tag="VE170_11360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ16078.1"
                     /translation="MGKTLERQCEEVIRIGPLGQHTDDVLRAKQIFYQYVVKKNYKAD
                     RTPKVLKSYADQVSACLKDASVDVVFSPGTIPISYLESSAPMVFWTDATVAGMIDFYP
                     GWSNLCNETLRDAHRMEQNALSNCRLAIYSSNWAAETAINNYRVDPDKVKVVPFGANV
                     ESVRDLNDIRKIVARRSMDVCKLLFIGSDWYRKGGDIAVEIAGSLNAMGLKTELTMLG
                     GHMAGAVPDFVSIRGFVSKKSPAGRTLIDRLFAESHFLLLPSRADCAPVVIAEAGSFG
                     VPVVASNVGGISTAVRNGINGYVLAGQLVTDACNVIFKSMESPSAYRQLACGSFGEYT
                     ARLNWPTAGRRVHELLKNILD"
     gene            complement(2292..3065)
                     /locus_tag="VE170_11365"
     CDS             complement(2292..3065)
                     /locus_tag="VE170_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16079.1"
                     /translation="MFFKNLPADGEYCNSKRIQLLKEPAEDREHRPYLAIIRCGAIHR
                     LIDDGSQRNFDIALSLYACPNDKRFLDGCEYSYCGGINKYKAACQFIDGALLEKFRGF
                     IFLDEDLEITYSHLSQFLDYCWAHGFGLAQPSLTSDSFYSHKHLLNASRSGWRPVGMV
                     EVMCPYFSSDSLRVALNTFDLSYSTWGLDHIWPRLLDVEPVVVDEFTIKHTTPVGGSD
                     SPFYKYMKRIGVSPQRERDKLRKLSGGEIASMAVIHLRP"
     gene            complement(3158..3973)
                     /locus_tag="VE170_11370"
     CDS             complement(3158..3973)
                     /locus_tag="VE170_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16080.1"
                     /translation="MDPDIERCFGRVDFVVQRHGKLIDQLTSRGDPLGIHVHPYRWNP
                     EQAVAFSDHGDSAWANHCLRVAADAFGNAFGRPARLSSHGGYFLTETLLDAAVELGIT
                     IDLTVEPGLAAKTADASFGAYATGPSTNFIDYPRHPYYPSRQALGVPSSSPADSRPIL
                     IVPLTSFDYQTALTPWHRRIADRLLDRPRRHSPLSAWRKWPSPKIYWDFVARAADEQQ
                     FRYFAFATRTDDPASSSHQNVWNLLEHLPDHPIAERLHFVDPLAFKIQPLATP"
     gene            5012..5851
                     /locus_tag="VE170_11375"
     CDS             5012..5851
                     /locus_tag="VE170_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ16081.1"
                     /translation="MPKAKLNDVELYYEEAGAGDAVLLCPPSWWPCDTWNVGVVPFLS
                     KKFRTLIFDCRGTGHSSKPDHGYTIEQFAKDCAGLLEHLKIARCHAAGFALGGQIAQA
                     LAIQRPDLAATLTIAAAGAGVKTTDGGLRVVRNTDEEEIRAHGFEQFIRGHIENTHMA
                     FDGKFFAEHQDVVKRLSDALWKRQTSPEQHRYHFDARRTWDTLGNAPRVKVPALILCG
                     AGDDVNRGGSTPVGTARKLGELVPGSELALVPNVKHMTFWDGDGALTVLQEFLARHPI
                     EKL"
     gene            5922..6983
                     /locus_tag="VE170_11380"
     CDS             5922..6983
                     /locus_tag="VE170_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16082.1"
                     /translation="MELRRFTVSAIAILMTLVIGGIGYAQDKGKGASDAVMQLYNAAK
                     KEGTVVIWGPTDAIIYQRAQEAMSKQFPGIKIEHFESIPEPLVQRIIAESQAGKPAAV
                     DIIQSGSLRALRPLIDRDMLASYPGWEKDFGLDAVYANQRFVGAYNLTLPIAYNTKLV
                     SAQDAPKNWEDLADPKWKGRKLIMEARLVPFAMLGTQWGKGKATELIKRILSQQQPLI
                     VQGGTTVANALAGGQVSIAVGTYAYTIERLKKQGAAVDWVAVSPLPVLNSAVGVLKTA
                     PHPNAARFFAGWLGSTEGQTVRYTNTGQAIEVGKNAIGNVAARIKTTQPAIILETENN
                     FAGILEIQRELGKLLGALR"
     gene            7174..8832
                     /locus_tag="VE170_11385"
     CDS             7174..8832
                     /locus_tag="VE170_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ16083.1"
                     /translation="MLVPLVMLFYASVKSTEDKLPFEATGMTLANYTTVLTSPSTLPI
                     FLNTFLFTAGSLAVGLPLAVLLAWLLERTAIPARRWIATFILVPMTVPSLLSAIAWIQ
                     LLDPRIGIVNVVLRNLLGLSGDFGPFDIYTLYGMCFVQGLRLVPSAYLMIAASFRAMD
                     PSLEEQSAMAGRGVGQTTLRITLPIMRPALLAMLIYYIIVVIESFDIPGLLGFTARIR
                     VLSTAIYWATHSEVGLPDYGLASALGALVLLVALLLMWAYQRLTAHQERFATITGKGY
                     RPRRVSLGAWTAPASALCLLYVALAVVLPFLMLIWTSVQPFYAVPSADSLARVTFDGY
                     VKIWQESTVMRALWNTLVIALVTSAATILLAVMISWFVVRRGRTADGLSHYLATVSFL
                     PQCVPSIVIGLAFIFVYVRFPIPIYGTLWIIALAMTTRYLAYSSRTMTSALMQVHGEL
                     EEASQMSRAPWARTLRKITLPLLAPAMINVFFWVAVHAMQELSMALMLYNPETVVVST
                     MIWSMWQNGRTTDAAVLGVILILLSAVLLLGGQLFAQLRRSADS"
     gene            8979..10073
                     /locus_tag="VE170_11390"
     CDS             8979..10073
                     /locus_tag="VE170_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16084.1"
                     /translation="MISVIDLQKSFAGARGEVRALRGVSFEVGKGELFTLLGPSGCGK
                     TTTLRCIAGLEQPLSGKIVIGGQVVFSAANGTFIPPERRNIGMVFQSYAIWPHMTVFQ
                     NVAYPLAKEANRIEIAERVGRILDRLSLGTLADRLAPNLSGGQQQRVALARALVAQPQ
                     VLLLDEPLSNLDAKLREQMRFELKSLQESFGITTVYVTHDQEEALALSDRIGLMHEGM
                     LLEVGAPKELYLRPAHRITADFLGTANFIAGHVELAGNAAWDEMLIATAIGKFAACRS
                     VEWQPGMAAELFFRPESIELMDESMADGPANHTSALVERVTFLGNSADVILRCGEVRI
                     RLRAHSARTPHVGSTVKLSVPADSCIVFPA"
BASE COUNT         2200 a         2694 c         3002 g         2317 t
ORIGIN      
        1 gggttgttgg cgcatctcgg catttcgcgc tgtcacgccg tcggcttcgc tctgggcggg
       61 caaatcatgc aggccctggc gatcgagcgg cccgatctag tggcgacgtt gacgattgcg
      121 gcttcgggag cgggcacgaa aactctcgcc ggcggacagc gaaatctttc gccgcacacg
      181 gaacgggaaa tccgcgccat cggtttcgag cgctatattc gcaactacat cgagaacgac
      241 acgatggcgt tcaatcggca attctaccgc gaccatggca acgtcgtggc agcgttggcc
      301 gatgcggtgt ggtcgggaca gagcacggtg gaaatatttc gccgccacga agatgcgcgg
      361 ctcacctggg atactctcgg gaaagcggcg gaagtcacgg tgccgacgtt ggtgctttgc
      421 ggcgccgacg acgatgtcgc ccggcgcggc agcacgccgg tcggaactgc ccgtcgtctc
      481 gccgagctga cgcctcgcgc tgagctgtgc ttgattccca acaccaagca catgactttt
      541 tgggatggca acggcgcatt ggtggcgttg caggaatttc tgctgcgcca tccgatcgcc
      601 aagagccact gagtgctgat tcagaccgag gtgtcgcgta gttgtgctgc taggttcctg
      661 aaattgatcg atgcagatgg cagagttctc gccgtgtatc ctgatggctc cgtagtctcc
      721 attgcaaaca gcgattctag aagcttacac ttcgataata accaacttgc cagcgaaatg
      781 aggctcaacc gcgccgaata tgatgcgcgg gagatgtgtt ccgtcgagct taatgctgcc
      841 acccgacaaa atcagtaagg ggcccaacta tgctataatc ttggctggcc tgccagatgt
      901 agccatggtc gcggcattat tgattggtac gcgcgggttt ttagcggctc ttagagatgg
      961 ttgtgtcttt ttggcagatg aagaggcgca tcggaaatcg aacaagccgg atgggcggcc
     1021 gcgtcaactc gcggggtgac atatcgaccc gggctcataa actactgagg tgcgttaaac
     1081 ccaacttgcg aaagggaggg catacgaaga ttgcgtacgt caccacatac gactctttgg
     1141 acgtcgagaa ctggtcgggt tcaggttact tcatgggtaa gacactcgaa cgccaatgcg
     1201 aagaagtaat tcgcatcgga ccgctagggc aacacacgga tgacgtgttg agagccaaac
     1261 aaatttttta ccagtatgtg gtgaaaaaaa actacaaggc agaccgcact cccaaggttc
     1321 tgaagtctta tgccgatcag gtctcggcgt gcctgaagga tgcctctgtg gacgtggtgt
     1381 tcagtccggg aacaattccc attagttatc tcgaatccag cgcacctatg gtattttgga
     1441 cagatgcaac cgttgcggga atgattgatt tctatccggg ttggtcgaat ttatgcaacg
     1501 agactctcag agatgcccat cgcatggagc aaaacgccct gtcgaattgt cgattagcga
     1561 tttacagctc caactgggcg gccgagacgg ccataaataa ctaccgggtg gacccggaca
     1621 aagtgaaggt cgttcccttt ggcgcgaatg tcgaaagtgt tcgcgatttg aatgatataa
     1681 gaaaaatcgt tgcgcgaagg tcaatggatg tatgcaagct gctttttatc ggcagtgact
     1741 ggtatcgcaa aggcggagac attgcggttg aaattgccgg atcattaaat gctatgggtt
     1801 tgaaaacaga gttgactatg ctcggaggcc atatggcagg ggctgtcccg gattttgtgt
     1861 cgatcagagg attcgtatcc aagaaatccc ccgcaggccg aactctgata gatcgtcttt
     1921 ttgccgagtc gcatttcctg ttgctgccat caagggcgga ttgcgcacct gtggtcattg
     1981 ccgaagccgg ttcatttggc gtgccagttg tcgccagcaa tgtgggaggc atctcaacag
     2041 cagtgagaaa cggcatcaat ggatatgtct tggcagggca attggttacc gacgcctgca
     2101 acgtaatttt caaatccatg gaaagcccca gtgcttatcg tcaactggcc tgcgggtcgt
     2161 ttggtgaata caccgcacga ctcaactggc caacagcagg gcgtcgggtg catgaactcc
     2221 tgaaaaacat cctcgattga attccgtctt cgaagtaatc cgttccaggc aagcggggag
     2281 tctggtcgcg gtcacggacg caaatggatt acggccattg aagcaatctc cccgcccgaa
     2341 agctttctca gcttgtccct ctcacgttgc ggcgaaacgc cgatgcgttt catatactta
     2401 taaaagggac tatccgaacc accaactggc gtggtgtgtt tgatggtgaa ctcatcaacc
     2461 acgactggtt ctacgtccag cagcctcggc caaatatggt ctagccccca ggttgaatag
     2521 ctcagatcaa aagtgttcag cgctactctt agggaatcgc tcgaaaaata agggcacatc
     2581 acctcaacca taccaaccgg gcgccagcca gatcgtgatg cattgagcag gtgtttatgg
     2641 gagtaaaaag aatccgaggt caagctgggt tgtgccaggc cgaacccgtg agcccaacaa
     2701 tagtccaaga actgacttaa atgggaatag gtaatttcaa gatcttcatc gagaaatatg
     2761 aaaccccgga acttctccaa aagtgcaccg tcgatgaact ggcaagcagc cttgtactta
     2821 tttattccgc cgcaatacga gtactcgcaa ccatccaaaa aacgcttgtc gttcggacat
     2881 gcatacaaac tcaacgcgat atcaaagttc ctttggctgc catcatcgat tagacgatga
     2941 atcgccccgc agcgaatgat tgcaagatat ggccggtgct cgcggtcctc ggcaggttcc
     3001 ttaaggagtt ggatgcgttt ggagttgcaa tattccccat cggcggggag attcttgaaa
     3061 aacaaatgaa atctaagtcg gttagcctta agaatgaaac tatcggcgat gttcctgact
     3121 agacgccaga ttcggttgag aatcagccgg aaaaatctca cggggtggca agcggttgaa
     3181 ttttaaatgc gagcgggtcg acaaagtgca atcgctcggc aataggatga tccggtaaat
     3241 gctcgaggag attccagacg ttttgatgtg agctggaggc aggatcgtca gttcgggtgg
     3301 cgaacgcgaa gtagcgaaac tgctgctcat ctgcggcgcg cgccacaaaa tcccaatata
     3361 tcttcgggct gggccacttt ctccaggcgc tgaggggcga atgccgccgg ggcctgtcga
     3421 gaagtctatc agcgattcgg cgatgccacg gtgtcagggc agtctggtag tcaaacgagg
     3481 tgaggggcac gatcaatatc gggcgcgagt cggccggcga ggatgaagga acaccgaggg
     3541 cctggcgtga cgggtagtaa gggtgccgcg gatagtcgat gaagtttgtc gagggcccag
     3601 tcgcgtacgc cccgaatgat gcgtcggcgg ttttcgccgc gagacccggc tcaacggtaa
     3661 ggtcgatcgt gatccctagt tcgacggcag cgtcgaggag agtttcggtg aggaagtacc
     3721 cgccatggct ggaaaggcga gcgggccggc caaaagcgtt cccgaaggca tccgcagcga
     3781 cccgcaggca atggttagcc caggcgctgt cgccatggtc ggagaatgcg acggcttgtt
     3841 cggggttcca tcgatacggg tggacgtgga tgccaagggg atctccccga gaagtcagct
     3901 gatcgattaa cttgccatgt cgttgaacca cgaaatcgac gcgaccaaaa caccgctcaa
     3961 tatcggggtc catgcgcagt agccaggtgg gacgtacgac actgcccgat cgacccctta
     4021 agcgatcgct cagcttatcg actagagcca ctgtcgtaac aaatccctcc caaggtcgtt
     4081 ggcccacggg aggttggctt tggtcaggct ccacatcgat acgccagacc gttggtattg
     4141 gcggcggttt gatcatcgag gagttttgga cgttcgcgat gatgccgcat ctaaaggctg
     4201 ctgaagcgtc gcgccattac tataccgaga cgttagtatg gcaccatacc aatagatgaa
     4261 cggggaattt aaacaccctt tttgctgacg gagttatatc gacgaacctg agagtagtca
     4321 attgcgcgag tgtttagtaa agttggcgcc gcgggggatt cacctatcct ggtcttcatt
     4381 tagaagcccg atggcctaac aatctttttc tgctggtcat atccgaatat ccggctcaga
     4441 tacatcgcca tcgaccgcgt tcgttctctt cgcagcctca atttgttttg agctcatgct
     4501 ccttcttccg accactccgg ctcctagggg tctaaaatgt aattgaactc tttttaggtt
     4561 tgttgtagcg gtagctgatg gcccgacccc aacggatcgg ggctcctagt gccccacggc
     4621 ggaattgtcg cgctcgccca taagttttta ttcgggcgcg gcaatcgccc gggaacgatc
     4681 aaagtgatct cgtgcatgtt tagaatccaa ccaacgccgc agccaagctt tatgacactt
     4741 cgcgattgac gatgaccgcc gcttagggta accggcgatg ccgagaaaaa accggcttaa
     4801 tactttgaac cgtcggtgca aagaaagcgt caccctgcgg atttgaaaaa agcgcatttt
     4861 tcgcgcgacc agtgaatggc tgcaatggtt tcctagaggg gaaacgatcg cgcgcgatcg
     4921 cgttatctgg attgagtcac acggatgcac agctcgctgt aatttatgcg aaagtcaaat
     4981 acaaataggc gcgttaaacg agaggatcta aatgccgaag gcaaagctta acgatgttga
     5041 actgtattat gaagaagccg gtgccggcga tgcggtgctg ctctgtccgc cgtcgtggtg
     5101 gccttgcgat acttggaacg tcggcgtggt gcctttttta tccaaaaagt ttcgcacgct
     5161 catcttcgat tgccgtggca ccggccattc gagcaagccg gatcacggct acaccatcga
     5221 gcagtttgcc aaggactgcg ccgggctgtt ggagcatcta aaaatagcgc gctgccacgc
     5281 cgcaggattc gctctgggcg gccagatcgc ccaggctttg gcgatccagc gtcctgacct
     5341 ggccgcaacc ttgaccatcg ccgccgccgg tgcgggcgta aagacgacgg acggcgggct
     5401 tcgggtggtc cgcaacaccg acgaggaaga gattcgcgca catggcttcg agcagtttat
     5461 tcgcggccat atcgagaaca cccatatggc gttcgacggc aagtttttcg cggagcatca
     5521 agatgtggtg aagcgtttgt ccgatgcgtt gtggaaacgc caaaccagtc cggagcaaca
     5581 tcgttatcac tttgacgcgc ggcgcacctg ggataccttg ggcaacgcgc cccgggtcaa
     5641 ggtgccggcg ttgatcctgt gcggtgccgg cgacgatgtg aaccgcggcg gcagcacgcc
     5701 ggtaggtacg gcgcgcaaac tgggcgagtt ggttcccggc tccgagttgg cgttggtgcc
     5761 caacgtcaag catatgacct tttgggacgg cgatggggct ttgacggtgt tacaggaatt
     5821 cttggcgcga catcccatcg aaaagctttg aacgcagagc cgtacgacgc tacggatgtt
     5881 gtcttaattc atttcgctta attggcgagg agctgtcgat catggaactt cgacgattca
     5941 ctgtgagtgc cattgccatt ctcatgactt tggtaattgg cggcatcggc tacgctcaag
     6001 ataaaggcaa aggtgccagc gatgcggtga tgcagctata taatgccgca aagaaagaag
     6061 gcacggtcgt gatatgggga ccgaccgacg caattatcta tcagcgtgcg caggaagcca
     6121 tgagcaagca gtttccgggt attaagatcg agcacttcga gagtatccct gaaccgctgg
     6181 tgcaaagaat tatcgctgag agtcaggccg gcaagccggc ggccgtagat atcattcaat
     6241 ccggctcgct gcgcgcgttg cggcctttga tcgatcgcga catgttggcc agctatcctg
     6301 gctgggagaa agatttcggg ctcgacgcgg tttatgccaa ccagcgcttc gtcggcgcct
     6361 ataatctaac cttgccgatc gcttataaca ccaagctggt aagcgcgcag gatgcgccga
     6421 agaattggga agatctcgcc gatccgaaat ggaaaggcag aaaactgatt atggaggcga
     6481 gattagttcc ttttgccatg ctgggtacgc aatggggcaa gggcaaagcg actgagttga
     6541 tcaagcgaat actttcgcag caacagcccc tgatcgttca aggcggcact accgttgcca
     6601 atgctctggc ggggggccag gtttccatcg ccgtcggtac ctatgcctac accatcgagc
     6661 gcttgaaaaa acaaggcgcc gccgtcgact gggttgccgt ttcgccgcta ccggtgctca
     6721 attctgcagt cggagtgctc aagaccgcgc cgcaccccaa cgctgcgcgc ttttttgccg
     6781 gttggctggg aagtaccgag gggcaaacgg tcagatatac caacaccggc caggcgatcg
     6841 aggtgggcaa aaacgcaatc ggcaatgtcg ccgcgagaat caaaacgact cagcctgcga
     6901 tcatcttgga gaccgaaaat aacttcgccg gaattctcga gatccagcgc gaattgggaa
     6961 aactgctcgg cgcgctgcga tgaataagac gagactgggg acccggcatg atgcgccccc
     7021 ggatggattg actgccggac tatttaactt gaaatgagtg tatcgccaac cacattcggt
     7081 cgcagtgctc agaccggggc ttggtcatgg acgcggacat tgtcggttcg ttcggttgct
     7141 tttggctgca cccttgccgt ggtctcttat cttgtgctgg ttccgctcgt gatgctgttt
     7201 tacgccagcg tcaaaagcac ggaagataag ttgcccttcg aagcgacggg catgacgctg
     7261 gcgaattaca ccacagttct caccagtcct tccactttgc cgatcttttt gaatacgttt
     7321 ttatttaccg ccgggtccct agcagtcggc ttgccgctgg cggtcctgtt ggcgtggctt
     7381 ttggaacgta ccgcgatccc ggcgcgccgg tggattgcta cgttcattct cgtgccgatg
     7441 acggttcctt cgctgctctc ggcgatcgcc tggattcagc tgctcgatcc cagaatcggc
     7501 attgtcaacg tggtcttgcg caaccttctc ggcttgtcgg gggacttcgg tccgttcgat
     7561 atttatacgc tctacggcat gtgctttgtt caagggttgc ggttggtgcc atcggcttat
     7621 ctcatgatcg cggcttcgtt tcgcgcgatg gacccgtcgc tggaagaaca aagcgctatg
     7681 gcaggacgcg gcgttgggca aacgaccctg cgcatcaccc taccgatcat gcggccggcg
     7741 ctgctcgcca tgttgatcta ctacattatc gtcgtcatcg aatccttcga tattcccggg
     7801 ttgcttggtt tcaccgcgcg cattcgcgtg ctcagcacgg cgatctattg ggcgacccac
     7861 tcggaagtcg ggttgccgga ttacggtctg gcttccgcct tgggagcgct ggtgttgctt
     7921 gtcgcgctgt tgcttatgtg ggcgtatcaa cgcttgacgg cgcaccagga aagattcgct
     7981 acgatcaccg gcaaaggata ccggccgcgg cgagtcagtc tcggcgcctg gacggcgccg
     8041 gcaagcgcgc tctgtttact ctacgtggcg ctcgccgtag tgctgccttt tttaatgctg
     8101 atctggacca gcgtccagcc gttctatgcg gtgccttcgg cggattcgct ggcgcgcgtc
     8161 actttcgatg gctacgtgaa aatctggcag gaaagcacgg tcatgcgcgc gctatggaat
     8221 actctggtga tcgcgttggt aacttcggcg gcgacgatct tgctcgccgt catgatcagc
     8281 tggtttgtcg tccggcgcgg ccgaacggcg gacggactgt cgcattatct tgccacggtg
     8341 tcgtttttgc cgcagtgcgt gccgagcatc gtcatcggtc tggcatttat cttcgtctac
     8401 gtccgctttc ccattccgat ttacggcacc ctatggatca tcgcgctggc gatgacgacc
     8461 cggtaccttg cgtacagctc acgcacgatg acctcggcgc tcatgcaggt gcacggcgag
     8521 ttggaagaag cgtcgcaaat gagccgggcg ccgtgggcgc gcaccttgcg caaaatcacc
     8581 ctgccgctct tggcgcccgc catgatcaat gtgtttttct gggtggcggt acacgcgatg
     8641 caggagttat cgatggctct gatgctctac aatccggaga cggtggtcgt ctcgacgatg
     8701 atttggagca tgtggcaaaa cggccgcacc acggatgccg cagtcttggg ggtgatcttg
     8761 attttgcttt ccgccgtctt gctgctcggc ggtcagctat ttgcgcaact tcggcgcagt
     8821 gctgactcct agaaagttgc gcggtatttt gatttcacag cgcccgaaac tatctcaccg
     8881 gcgaagccgg gtcctggccg tcttcgttga tctgttcgat accatgagcg agatcaccgc
     8941 gcacggcaac gatcgattat ctgcaaaccg gatcattcgt gattagcgta atagatctgc
     9001 aaaagtcttt tgccggcgcg cggggcgaag tccgcgcctt gcgcggcgta tccttcgaag
     9061 tgggcaaggg cgaattgttt acgctgctcg gcccgagcgg ctgtggcaag acgacgacgc
     9121 tgcgttgcat cgccggattg gaacagccgt tgtccgggaa aattgtcatc ggcggtcagg
     9181 tggtttttag cgccgccaac ggcaccttta ttcccccgga gaggcgcaac atcggcatgg
     9241 tttttcaatc ctacgcgatt tggccgcaca tgacggtgtt ccagaatgtc gcctatccat
     9301 tggccaaaga ggcgaatcgg attgagatcg ccgagcgcgt cggacgaatt cttgaccgat
     9361 tgagcctcgg gacgctggcg gatcgacttg cgccgaacct gagcggcgga caacagcagc
     9421 gcgtggcact ggcgcgcgcg ttggtcgcgc aaccccaggt gctgttgctc gacgaaccgc
     9481 tcagcaatct cgacgcgaaa ttgcgcgagc agatgcgctt cgagttgaag tcgctgcaag
     9541 aatctttcgg catcacgact gtgtacgtca cccatgacca agaggaagcg ctggcgctgt
     9601 cggatcgcat cgggttgatg catgaaggca tgctcctgga agtgggtgct cctaaggagc
     9661 tgtatcttcg cccagcgcat cgaatcaccg ctgattttct cggcacggcg aattttatcg
     9721 ccggtcacgt cgagctggcg ggcaatgccg cttgggatga aatgttgatc gcgactgcga
     9781 ttggcaagtt cgctgcatgc cgcagcgtcg aatggcaacc tggcatggcg gcggagctgt
     9841 tttttcgacc ggaaagcatc gagctgatgg atgagagtat ggccgatgga ccggcgaatc
     9901 acacttcggc cctggtcgaa cgggtgactt tcctcggcaa ctcggccgat gtgatattgc
     9961 gctgcggcga agtgcgcatt cgcctgcgcg cccactcggc gcgcacccct catgtcggtt
    10021 ctactgttaa gctttctgtg ccggcggatt cttgtatcgt gtttccggcg taagatgttt
    10081 atttataccc gccccgtcgc gatgggcgtg gctagggtga gtttcctttc gcacagtgag
    10141 aggggaagaa agaagggaga atgacgactg aatcatcgat tgtagaacga cgcgagcagt
    10201 ctaataccat cga
//
LOCUS       DASQKS010000442         6038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5694555, whole genome shotgun sequence.
ACCESSION   DASQKS010000442 DASQKS010000000
VERSION     DASQKS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5694555"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="VE170_11395"
     CDS             <1..1601
                     /locus_tag="VE170_11395"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HYJ16085.1"
                     /translation="AKKKTDREKYIDEVISVIGKKLEAEGIDAEVTGRPKHFYSIYQK
                     MESQNLLYDQIYDLVAFRILVDTPRECYETLGVVHAQWKPVPTRFKDYIALPKPNMYQ
                     SLHTTVIGPYGERIEIQIRSHEMHRVAEEGIAAHWRYKEGEAFQVSDIQRFSWLRQLL
                     EWQENLQDPQEFLHSLKEDLFAAGMYVFTPKGDLLHFPRGSTVVDFAYRIHTEVGNRC
                     SGARVNGQLVSLKYILRSGDTVEIITTPQQVPSREWLKWVKTPRAKSKIRNWLKTQQH
                     ERSVVLGREIFDSDLHRYRLDYGQLKREGKIDWLSHELGMKDEEALLSAIGYGRITTR
                     HAIVKLVPADKLDSGSKKAGGALESLFKLVSRQKRSLGIRVKGVDDVLVRFALCCHPL
                     PGEHIVGFITRGRGVTIHTVGCTTVLESDPHRKIEVAWEESGQPPRPVKIEVTCVDQP
                     GLLAAISTAITSADANIARAQVRTFSSQRAVNTFEVMIKSSEHLKEVLLNISKVKGVY
                     KAIRGRGRAGSDIESDGTVKEELH"
     gene            complement(1612..1803)
                     /gene="rpmB"
                     /locus_tag="VE170_11400"
     CDS             complement(1612..1803)
                     /gene="rpmB"
                     /locus_tag="VE170_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYJ16086.1"
                     /translation="MARICKICGKGRSVGMNVSHANNKTKRSWGANLKTVRGKFGGQV
                     KRLLVCTDCIHSGRIQKVA"
     gene            complement(1796..2275)
                     /gene="rfaE2"
                     /locus_tag="VE170_11405"
     CDS             complement(1796..2275)
                     /gene="rfaE2"
                     /locus_tag="VE170_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HYJ16087.1"
                     /translation="MPEKLKSLGQLAAIAAAARANGQTVVFTNGCFDLLHRGHVHTLR
                     QAKAAGDLLIVAVNSDRSVKSIKTAQRPILPETDRIELIAAMEMVDYVILFDDPDPYQ
                     LIAAIKPQVLAKGGDWSSAEIIGADIVQAAGGRVALIPYLKGFSTTEIIERIRNLDG"
     gene            2434..3870
                     /locus_tag="VE170_11410"
     CDS             2434..3870
                     /locus_tag="VE170_11410"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16088.1"
                     /translation="MEYQDLRDWIQIADEMGELKTLKGCDWNLEIGAITEIVAHKEDG
                     PAVLFEDIKGYPKGYRVLSNSLSSRKRLALTLGLPAGDTKMDFVRIWKENYKKVKPIP
                     PKFVKKSGLFDNVYRDGDIDMFKFPTPKWHDKDGGRYIGTGSVDITRDPDEGWVNYGT
                     YRVMIHEKDKVGFYISPGKHGRIQREKYQASGAPFKIAMSFGHDPLTFLAGSIEVPYG
                     VPEYEFIGGIRGEPFEMIEGEYSGLPIPANAEIVIEGDVHFDQPKVEGPFGEWTGYYA
                     SAERAEPWVEVKRLYHRNDPIILGSPPGRPPAELGWYRSYLRSALIWDEMEKAGVPDV
                     KGVWLTVSGGSRLMMCVSIKQRYPGHAKQAAVIASQCHAGAYLGRFVIVVDDDIDPSN
                     TDDMIWAMSSRCEPEADIDILRRCWSGPLDPVIHPTKKGFNSRAIIDATRPYEWIKDF
                     PPVAESSREVLDATAKKWGAELFGNGKK"
     gene            4004..5785
                     /gene="mutL"
                     /locus_tag="VE170_11415"
     CDS             4004..5785
                     /gene="mutL"
                     /locus_tag="VE170_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HYJ16089.1"
                     /translation="MKIHILSEAMASRIAAGEVVERPASVVKELMENSLDAGATEIFV
                     WLEKSGTVVIRITDNGEGMTAEDLALAVERHATSKLREDDDLFRITTLGFRGEALPSI
                     GSIAKMDISSRPASSSIGTKLTVDGGNRGEIHAAAAAVGTTIEIRDIFYNTPARRKFL
                     KAPATELSHVCDVFNRLALANPQVHFRLQHDGRTVADYLSVQQGRDRLQQVLGVDVAK
                     GLKPFTFTQDEVRVSGFLSTAPTSFPNARYLFTFVNRRYVRDKVITHALLHGYDTLLM
                     KGQYPAVVLFLEVPFAEVDVNVHPAKYEVRFRRQSDVHETVSRSVRSALQRDAKAPGA
                     VVAMSGYAASPGEWRPAYSFAPAADPTVRESILGHSIHEYPNVESMPSRDVTFTVPQN
                     ERPGRVGFFSSLTIHDQILGCYLVCSATDRLILIDQHAAHERIAFERLRHQLRANAVE
                     RQDLLIPQSVELSAGEMLMLQQKLAVMERHGFCLEPFGPSSFAIMSVPALLPEGDYTQ
                     LVRQMVAELAEVDTSARLRQHLEDRLATMACHSVIRANRKLGMEEMRALLCDLDQVEF
                     ATQCPHGRPVLVELSRDQLERMFKRIA"
     gene            5898..>6038
                     /locus_tag="VE170_11420"
     CDS             5898..>6038
                     /locus_tag="VE170_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HYJ16090.1"
                     /translation="MKPKIVIILGPTAVGKSALALELAESLNGEIVNADSQQVYRYLD
                     IGT"
BASE COUNT         1310 a         1648 c         1752 g         1328 t
ORIGIN      
        1 tcgccaagaa aaaaaccgac cgggaaaagt atattgacga agtgatttcg gtaatcggca
       61 agaagttgga agccgaaggg atcgacgccg aagtgacggg ccggcccaag cacttctaca
      121 gcatctacca aaagatggag agccagaacc tgctttacga tcagatctac gatctggtcg
      181 cttttcgtat tttggtcgac acgccgcggg agtgttatga gaccttgggg gtggtgcatg
      241 cgcagtggaa accggtgccg acgcgcttta aagactacat cgcactaccc aagcccaaca
      301 tgtatcagtc cctgcatacc acggtgatcg gtccctacgg tgagcgcatc gagatccaga
      361 tccgctccca tgagatgcat cgggtcgccg aggaagggat cgcggcgcat tggcgctaca
      421 aggagggcga ggcctttcag gtgagcgata tccagcgctt ctcgtggctg cgccaattac
      481 tcgagtggca ggaaaatttg caggacccgc aggagtttct tcacagcctt aaggaagacc
      541 tgtttgccgc cggcatgtat gtctttaccc cgaagggcga tcttttacac tttccgcgcg
      601 gctccacggt tgtcgatttt gcctaccgga ttcataccga ggtcggcaac cgctgttccg
      661 gagcccgcgt caacggccaa ctggtgtcgt taaaatatat actgcgcagc ggcgacacgg
      721 tggaaatcat cacgacacca cagcaagtgc cgagccgtga gtggctcaag tgggtgaaga
      781 cgccgcgggc gaaatcgaaa atccgtaatt ggctcaagac ccagcagcac gagcgcagcg
      841 tggtgctggg ccgcgagatc ttcgacagcg acctgcatcg ttaccggttg gactatgggc
      901 agctcaaacg ggaaggcaag atcgactggt tgagccatga gctcggcatg aaagatgagg
      961 aagcgctgct atcggccatc ggttacggcc ggatcaccac gcgccatgcc atcgtcaagc
     1021 tggtgccggc cgataaactc gacagcggca gcaaaaaggc cggcggcgcg ctggagtcat
     1081 tgttcaagct cgtctccagg cagaagcgca gtctcgggat tcgcgtcaaa ggcgtcgacg
     1141 atgtgctggt tcgcttcgcg ctttgttgtc atccgctacc cggcgaacat atcgttgggt
     1201 ttattacccg gggccggggt gtgacgattc ataccgtcgg ttgcacgacg gtcttggaaa
     1261 gcgatcctca tcgcaagatc gaagtcgcct gggaggaaag cgggcagccg ccgcggccgg
     1321 tcaagatcga agtgacctgt gtcgatcagc ccggcttact ggcggcgatc agtacggcga
     1381 tcactagcgc cgatgccaat atcgcccggg cccaggtaag gacattttcg agccagcggg
     1441 cggtcaacac cttcgaggtg atgatcaaaa gttcggagca cttgaaggaa gtgcttctga
     1501 acatttcaaa agtaaaagga gtctacaaag cgatacgcgg gcgtggccgg gccggcagtg
     1561 atatcgagtc cgatggaacg gtcaaggagg agttacactg aacaagagtc gctaggcgac
     1621 cttctggata cgacccgagt ggatgcaatc ggtgcacact agcaagcgtt tgacctggcc
     1681 gccgaacttg ccccgcaccg tttttaggtt cgctccccag gagcgtttag tcttgttgtt
     1741 ggcatggctg acgttcattc cgaccgaacg gcctttgccg caaattttgc aaattctagc
     1801 catctaaatt ccttatcctt tcgattatct cggttgtcga aaatcccttc aagtacggta
     1861 tcagtgcgac ccgtccaccg gccgcctgga cgatatcggc gccgatgatt tcagcgctgc
     1921 tccaatcgcc gcctttggcc aaaacctgcg gcttgatcgc ggcgatcagt tggtacgggt
     1981 ctggatcgtc aaaaagaatg acgtaatcta ccatctccat ggcggcgatc aactcaatcc
     2041 gatcggtttc cggtaaaatc ggccgttgcg ccgtcttgat cgacttcact gatcggtcgc
     2101 tgttgaccgc gacgatgagc aaatcgccgg cggctttggc ttggcgcagc gtgtgcacgt
     2161 ggccgcggtg cagcaaatcg aaacagccgt tggtgaaaac caccgtttgc ccattggcac
     2221 gcgccgctgc ggcgattgcc gccaattggc ccaagctttt caatttttcc ggcacaatct
     2281 tggggtaaca tgataagtcg ttgttttcaa ttaaattttg cctagctgcg taccggcacc
     2341 ggcttgaatc agcgggagaa tttcgttaag gtcgtcggca agttgtttcg gtcaccaacg
     2401 accttcggtt gtgaattttt taggaggaaa atcatggaat atcaggatct gcgagactgg
     2461 attcaaattg ccgatgagat gggtgagctc aagacgctca agggctgcga ttggaatctg
     2521 gaaatcggcg cgattactga aatcgtcgca cataaggaag atggacctgc ggtccttttc
     2581 gaggacatca agggctatcc caagggttat cgggtgctat ccaattcgct gtcgtcacgc
     2641 aagcgcctcg cgctgaccct aggcttgccg gccggcgata ccaaaatgga tttcgtcagg
     2701 atctggaaag agaactacaa gaaggtcaaa ccgatcccgc ctaagttcgt caagaagagc
     2761 ggactgttcg acaatgtcta tcgtgacggc gatatcgaca tgttcaagtt tcccacgccc
     2821 aaatggcatg acaaagacgg cggccgttac atcggtaccg gcagcgtcga tatcacccgc
     2881 gatccggacg agggttgggt caactacggc acctaccgcg tcatgattca tgagaaggac
     2941 aaagtcggat tttacatctc tcccggcaag catgggcgca ttcaacggga aaaatatcag
     3001 gccagcggcg caccttttaa aattgccatg tccttcggcc atgacccgtt gacgtttttg
     3061 gccggtagca tcgaagtgcc ctacggcgtg ccggaatacg aatttatcgg cggcatccgc
     3121 ggcgagcctt ttgaaatgat cgaaggcgag tactccggct tgccgatccc agccaacgcc
     3181 gaaatcgtca tcgagggcga cgtccatttc gatcaaccca aggttgaagg tcccttcggt
     3241 gagtggaccg gttattatgc cagcgctgaa cgcgccgagc cttgggtcga agtgaagcgt
     3301 ttgtatcatc gcaacgatcc gatcattctc ggttcccctc cgggccgtcc gcccgcggaa
     3361 ttgggctggt accgttccta tctccgctcc gcattgatct gggatgagat ggaaaaagcc
     3421 ggcgtgcctg atgttaaagg cgtctggctc acggtctccg gcggcagccg cttgatgatg
     3481 tgtgtatcga tcaaacagcg ctaccctggc catgccaagc aggccgcagt gatcgcctcg
     3541 caatgtcacg ccggtgcgta tctcggccgt ttcgtgatcg tcgtcgacga tgacatcgat
     3601 ccttcgaata ccgacgacat gatctgggcg atgtcgagcc ggtgcgaacc cgaggcggat
     3661 atcgacatcc tgcgccgttg ctggagcgga ccgctcgatc cggtcattca tccgaccaag
     3721 aagggtttca actcgcgggc gatcatcgac gccacccggc cctatgagtg gattaaggac
     3781 tttccaccgg tcgccgagtc gtcccgggaa gtgctcgacg ccaccgcaaa aaagtggggc
     3841 gccgaactgt tcggcaacgg caagaagtaa ttaaatttct ttaccgtgaa aagggcaagc
     3901 ggctaaatgc cggcttgccc tttttatttt ttggcgcatc gatcgagccg ttgacccaat
     3961 cttttgccct gggttatggt ttcatacgga gccaagacaa aatttgaaga tccatattct
     4021 atctgaggcc atggccagcc ggatcgccgc cggcgaggtg gttgagcggc ccgcgtcggt
     4081 ggtcaaagag ttgatggaga actctcttga cgccggcgcc acggaaatct ttgtttggct
     4141 cgaaaagagc ggcaccgttg tcattcgcat caccgacaac ggcgagggga tgacggcgga
     4201 ggatttggcc ctcgcggtgg aacgccacgc caccagcaaa ctgagagaag acgacgatct
     4261 gttccgcatc acgacattgg gatttcgcgg cgaggcgttg ccgagcatcg gctccatcgc
     4321 caagatggac atttcgtctc gcccggcgtc tagctctatt ggcaccaaat tgactgtcga
     4381 cggcggtaat aggggcgaaa tacacgccgc tgcggctgcc gtgggcacga ccatcgagat
     4441 tcgcgatatc ttttacaaca ccccggcgcg acgcaagttt cttaaagcgc cggcgacgga
     4501 gttaagccat gtctgcgatg tattcaaccg tttggccctg gctaacccac aggttcattt
     4561 tcgccttcaa catgacggcc gcacagtggc ggattatctt tcggtgcagc agggacggga
     4621 ccgtttgcag caagtactgg gtgtcgatgt ggccaagggc ttgaagcctt tcaccttcac
     4681 ccaggatgag gtgcgcgtca gcggtttcct gagtaccgcg ccgacctcat ttcccaacgc
     4741 tcgttacctg tttactttcg ttaaccgccg ctatgtgcgt gacaaagtga ttacccatgc
     4801 gctgctgcac ggctacgata ctctgctaat gaaaggccaa tatccggcgg tggtgttgtt
     4861 cttggaagtg ccgttcgccg aggtcgatgt caatgttcat ccggcaaagt acgaagtgcg
     4921 cttccgtcgc caaagtgatg tccatgaaac ggtgtcgcgg tcggtgcggt cggcgttgca
     4981 gcgcgatgcc aaagcgccgg gcgctgttgt agcgatgtcg ggctacgcag cttcgccggg
     5041 cgaatggcgg ccggcgtatt cctttgctcc cgccgccgat ccaactgtac gcgagtcgat
     5101 tttgggccat tcaattcatg agtacccaaa tgtcgaatcg atgccgtcgc gcgacgtgac
     5161 gtttacagtg ccgcagaacg agcggccggg gagggtcggt ttcttctcat cgctcactat
     5221 ccacgatcag atccttggct gctatctggt ctgctcagcc acagaccggt tgatcctgat
     5281 cgatcaacac gccgcccatg aaagaatcgc cttcgaacgg ctgcgccatc agttgcgggc
     5341 caacgccgtc gagcggcagg atctactgat tccccagagc gtcgaactta gcgccggtga
     5401 gatgctgatg ctccagcaga aattggccgt tatggaacgt cacggatttt gtttggagcc
     5461 gtttgggccg agttccttcg caattatgtc tgtccccgca ctgctgcccg agggcgacta
     5521 cacccagttg gtacgccaga tggtcgccga gttggctgag gtcgatacct cggccaggct
     5581 gcgtcagcac ctcgaggatc gtttggcgac gatggcttgt cacagcgtga ttcgcgccaa
     5641 ccgcaaatta gggatggaag agatgcgcgc gctcctgtgc gatctcgacc aggttgagtt
     5701 cgccactcaa tgcccccatg ggcggccggt gttggttgag ctgagccggg accagctgga
     5761 gcgcatgttc aaacgcatag cgtaatactc tcgttgaagg ttcaagggtt caacaattca
     5821 acctcagttt cagagttcgg acaaaagctt tgaacgcttg aacggttgaa cttcgaacgg
     5881 atgttatctt taattcgatg aagccgaaaa tcgttatcat ccttgggccc acggcggtgg
     5941 gcaagagcgc gttggccttg gaactggccg agtcattgaa tggcgagatc gtcaatgccg
     6001 actcccagca agtttaccgt tatctcgata tcggcacg
//
LOCUS       DASQKS010000443         6373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5271655, whole genome shotgun sequence.
ACCESSION   DASQKS010000443 DASQKS010000000
VERSION     DASQKS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5271655"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VE170_11425"
     CDS             <1..270
                     /locus_tag="VE170_11425"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HYJ16091.1"
                     /translation="GKSTSKNRFGAHQPAEQVYGNTLYVTWFAGGLRAVDFSNPYQPK
                     ETGYYVPTPGRGQSVVMSNDVFHDKDGRLYLIDRYDGLEILESQV"
     gene            399..992
                     /locus_tag="VE170_11430"
     CDS             399..992
                     /locus_tag="VE170_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ16092.1"
                     /translation="MKLYNVAYSGNSYKVRLLLAQLDIPCEIVEVDILHGASRTPEFL
                     KINPNGRTPVLDDNGFILAESNAILAYLARATKYLPDDREKFGQVFQWMFFEQYSHEP
                     FIATSRFWLQHKPDTAKRAALLAAKRDGGWAALNIMEDHLAKNDFFVGDYSIADIALF
                     AYTHVADEGGFLLSDFPKIRGWIGRVTTQFRFLPMSA"
     gene            complement(1087..2796)
                     /gene="ybaL"
                     /locus_tag="VE170_11435"
     CDS             complement(1087..2796)
                     /gene="ybaL"
                     /locus_tag="VE170_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HYJ16093.1"
                     /translation="MPHNVALITTLVAALGAALAFGFLAARFKLPVLVGYLAAGIVIG
                     PFTPGFVADAELSAQFAEIGVMLLMFGVGLHFSIDELLAVRKIAVPGAIVQITVATLL
                     GLALSNLWGWKLAEGLVFGLTLSVASTVVLLRALESRGILETVNGHIAVGWLIVEDLV
                     TVLVLVLMPPLSLWLGGSGGGQVTGQDLFTALALTLGKVALFVGFMVIGGRRLVPWIA
                     WHIARVGPRELFTLCVIAIAMGIAYGAAELFGVSFALGAFFAGMVMRESPLSHRAAEE
                     SLPFRDSFSVLFFVSVGMLFDPQVLIHEPLRIVAVVAIIVIGKSLAAFVLVLLFGYPL
                     NTALTVSAGLAQIGEFSFILAGLGMSLGILPEAARSLILAGAIISIAVNPLVFHAIEP
                     LQVWIRAKSKRAQLFERSEDPLATLPMTTDLNRLSGHVVIVGYGRVGRRIGAALAAQG
                     ISFVLAEENREIIQAAREQGIAAVSGDATEPMVLVQAHIHRARLLVIATPDTLNVRRM
                     IEIARTVNPRIETVVRTHSEQEAELLEKEGAGKIFLGEHELATSMSQYVLGRYAVKVE
                     QAS"
     gene            complement(2927..3826)
                     /locus_tag="VE170_11440"
     CDS             complement(2927..3826)
                     /locus_tag="VE170_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYJ16094.1"
                     /translation="MPELPDITIYIEALESRLLNQPLEKIRLASPFLLRTFEPPIGSA
                     EGKKIVGFQRIGKRIVIELEDELFLVFHLMITGRFHWKKRGIAVPRKYGHAAFDFSMA
                     TLLLTEMGTKKRASLHLVRGREHLQEHDPGGLEIFAADLASFRAALTLENHTLKRTLT
                     DPHLFSGIGNAYSDEILHRAKLSPVKQTKLLSDDEVDRLFHATRACMAEWIERLRRDT
                     GKNFPERVTAFRDDMAVHGKYRKPCPVCGAPVQRIVYAENETNYCAKCQTGGKLLADR
                     SLSRLLKDDWPRTLEEMEEGRRS"
     gene            complement(3931..5352)
                     /locus_tag="VE170_11445"
     CDS             complement(3931..5352)
                     /locus_tag="VE170_11445"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HYJ16095.1"
                     /translation="MSEEILNQLAVDMTALLTEPAGSRRVKITDLTPRDGQQCKLATR
                     VTTDDLLPLCASIDNCGFYAVEVWGGATFDVCMRYLNEDPWQRLRRIKAVMPKTKLQM
                     LLRGQHILAYRPYTDKIVYKFVERAVANGINVFRVFEATNDFRNIETATKAVKEFGGE
                     AHVEVNYTVSPVHTLEKWLEYAEQLIDVGADWLSLKDATGILTPFNAYRIIKGIKDRA
                     KGNLPVLLHCHDMGGTSSMCHLMAVLAGVDMIDTVLSPLSFGSAHPATETMVASLAGT
                     PFDTGIDLKVLEEPSLITRQIKEKYEKYETGYTGVSAEVFIHKVPGGMISNMVAQLKE
                     AKRMDLMDAALKETPSVEKDLGHPPLLTPTSQIVGVQAVLNVLAGERYKIVTKETQDY
                     VKGKYGRPPGTVNPELIKKIMKDKQPDYSIRSGEMADPADWDKAIEALGPLAKCEEDL
                     LLGVLFPMQAKEFLTKRENVIQN"
     gene            5655..6002
                     /locus_tag="VE170_11450"
     CDS             5655..6002
                     /locus_tag="VE170_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16096.1"
                     /translation="MKIVRLYTGADNESHFEDIEVEMTAQGVDQVSELQPAHGVMFRS
                     APLSHRSGYHTVPRRQYVITLSGQVEIETGDGTVRRFGAGDVMLAEDTTGRGHMTRVV
                     GDQPRHYVFIPLK"
     gene            6081..>6373
                     /locus_tag="VE170_11455"
     CDS             6081..>6373
                     /locus_tag="VE170_11455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16097.1"
                     /translation="MPSMMVATLGIALPEIRQTFSLTELQAGSLFSVMMIIAAVTSAI
                     AGRLADRIGRKTVLITGLSLLASGFGCAGISGDPLLFFLLLAVTGVGYGFTPPS"
BASE COUNT         1426 a         1866 c         1690 g         1391 t
ORIGIN      
        1 ggaaaatcga cgtccaagaa ccgcttcggc gcgcaccagc cggctgagca agtttacggc
       61 aacaccctct acgtcacctg gttcgccggc ggcctgcgcg ccgtggattt ttccaatccg
      121 taccaaccca aggaaaccgg ctactacgtc cccactcccg gcagaggtca gagcgttgtc
      181 atgagtaacg atgttttcca cgataaagac ggcaggcttt atttgatcga tcgctatgac
      241 ggactggaaa tactggaaag tcaggtttaa gcgaagcttc aaccgttcaa aggttcaaaa
      301 gttcaaggtt caatcggaca acgccaccgt agtcattttg tacaagagag cctctgacct
      361 tgaacctttg aaccttgaac tcttgaacgg tgttttccat gaaactctac aatgtcgcct
      421 actccgggaa ctcctacaaa gtccgcctgc tgcttgcgca gttagacatc ccctgcgaga
      481 tcgtcgaagt cgatattttg catggtgcaa gccgaacacc tgagtttctc aagatcaacc
      541 ccaacggccg cacaccggtg cttgacgaca atggattcat cctggccgaa tccaacgcca
      601 tcctcgccta cctcgcccgt gccacgaaat atctccccga cgatcgcgaa aaattcggcc
      661 aggtatttca gtggatgttc ttcgagcaat acagccacga gccgtttatc gctacgtcgc
      721 gcttctggct ccagcacaaa cccgacactg ccaagcgcgc cgcgttgcta gcagccaaac
      781 gcgacggcgg ctgggccgca ctcaacatca tggaggacca tctggccaag aacgatttct
      841 tcgtcggcga ctattccatc gccgacatcg cgctgttcgc ttatactcac gtcgcggatg
      901 aaggtggctt tttactgagt gattttccaa agattcgtgg atggatagga cgagtaacaa
      961 cgcagtttag atttttgccg atgtccgcct agcaagaaat ccgcggcaat ccagtctgac
     1021 attgttgccg tgagatcctg caacaacatt tctaatcgcc gctagttgcc aagcccatgg
     1081 taattcttag cttgcctgct cgactttaac cgcatatctg ccgagcacgt attggctcat
     1141 gctggtagcc aattcatgtt ccccgaggaa aattttgccc gcgccttcct tctccagcag
     1201 ttccgcctct tgttcgctgt gggtacgcac cacggtctcg atgcgcgggt tgacggtgcg
     1261 ggcgatctcg atcatgcggc gaacgttcaa ggtgtcgggc gtggcgatga ccagtaaccg
     1321 cgcgcggtgg atgtgcgcct gtaccagcac catcggctcg gtcgcatctc ccgacacggc
     1381 tgcgattcct tgctctcttg ccgcctggat aatttcacga ttttcctcag caagcacaaa
     1441 cgagatcccc tgcgcggcta gcgcagcccc gatgcgtcgc cccactctgc cgtagccgac
     1501 gatcacaaca tggccggaga gtcgattcag gtcggtggtc atcggtaatg tcgcgagtgg
     1561 atcctcggaa cgttcgaata gttgggcgcg tttagatttt gcccgaatcc atacctgtaa
     1621 aggctcgatc gcgtggaaga ccagcggatt gacggcgatc gaaataatcg ctccggcgag
     1681 aattagactt ctagccgctt ccggcagaat gcccagggac atccccaatc cagctaggat
     1741 aaaagaaaac tcaccgatct gagctagacc tgcggagacg gttagggcgg tattgagcgg
     1801 ataaccgaag agcaagacca aaacaaaagc cgcgagagat ttgccgataa ctataatcgc
     1861 cacgaccgcc acaatacgca gcggttcgtg aatcagcacc tggggatcaa agagcatgcc
     1921 gaccgaaacg aaaaacaaaa ccgaaaaaga gtctctgaaa ggcaacgact cctccgctgc
     1981 ccggtgactc aatggcgact cgcgcatgac cattcccgcg aagaatgcgc cgagggcgaa
     2041 cgagaccccg aagagttcgg cagcgccgta ggcgatcccc atcgcgatgg caatgacgca
     2101 caaggtgaaa agttcgcgcg gtccaacccg tgcgatatgc caggcgatcc agggaaccag
     2161 acgtcgcccg ccaatcacca taaagccgac aaaaagcgcg accttgccca aggtcaacgc
     2221 gagggcggtg aacaagtctt gaccggtaac ttgaccgccc ccggaaccgc cgagccacaa
     2281 tgaaaggggc ggcatcagaa ccaataccag caccgtgacc aaatcctcga cgatcaacca
     2341 accgaccgca atgtggccgt tgaccgtttc cagaatgccc cggctttcca aggctctgag
     2401 gagcacaacc gtactcgcga ccgataatgt cagtccgaaa acgagcccct cggcgagctt
     2461 ccaaccccaa agattgctaa gggcaagtcc aagcagtgtc gcgacggtga tctgcacgat
     2521 cgctccaggt acagcaatct tgcgaaccgc cagcagctcg tcaatggaga aatgcagtcc
     2581 cacaccgaac atgagcagca tgacgccaat ttcggcaaac tgggccgaaa gttcggcgtc
     2641 cgcgacaaag ccgggtgtaa atggaccgat gacaatgccg gcagcgaggt agccgaccag
     2701 aaccggcagc ttgaaccgcg cggccaaaaa gccaaaagcc aatgccgcgc caagcgcggc
     2761 aacaagcgtc gtaatcaggg caacgttgtg tggcaagtgc cggactccat tcgttacgat
     2821 caaagatccg cgcttggatg aagacaacta acacctgcgc agtgagtaaa cacaggcgaa
     2881 aagatacaac attggcgagc tagggcaaca tggcgcaatc gattaattag gaacgcctcc
     2941 cttcctccat ctcctccaac gttcgtggcc aatcgtcttt gagcagacgc gacaagctac
     3001 ggtcggcgag caacttgccg ccggtctggc acttggcgca gtagttggtc tcgttctccg
     3061 cgtagacgat ccgctgcacc ggggcgccgc acaccggaca cggtttgcgg tacttgccgt
     3121 gcacggccat gtcatcgcgg aaggcagtca cccgttcggg gaaattcttg ccagtatctc
     3181 tgcggagacg ctcgatccat tctgccatgc aggcgcgcgt tgcatggaag agtcggtcga
     3241 cctcgtcgtc gctcaatagc ttggtctgct tcaccggcga cagcttggcg cggtgcagga
     3301 tttcgtccga ataggcgtta ccgataccgc tgaacaaatg gggatcggtc aaagttcttt
     3361 ttagcgtatg attctcgagc gtcagcgctg cgcgaaaaga cgccagatcg gcagcaaaaa
     3421 tctccaggcc cccgggatca tgttcctgga gatgctctcg gccccgaacc agatgcagcg
     3481 aagcccgctt cttcgtcccc atctccgtga gcaacaaagt agccatggaa aaatcgaaag
     3541 ctgcgtggcc gtacttgcgc ggcacggcaa tgccgcgctt tttccagtga aacctgccgg
     3601 tgatcattag atggaaaacc aggaagagct cgtcttcgag ttcgataacg atgcgcttgc
     3661 cgatgcgctg gaatccgacg atttttttac cttcagcaga accaattggc ggctcgaagg
     3721 tgcgcaagag aaacgggcta gccagtcgaa ttttctccag aggctgattt agtaatcgtg
     3781 actccaacgc ctcaatgtag atggtaatgt caggaagctc cggcatattt aaaatggggc
     3841 gaggattgag actggagaat agagcatcgc tatcctcgat cctctatcgt ccatcctcga
     3901 gtttattcgc gccctttgcg ttctatgcgg ctaattctgt attacgtttt ctcgcttggt
     3961 caaaaactcc ttcgcctgca tcggaaacaa cacgcctaga agcaaatcct cttcgcactt
     4021 cgccaacggt ccgagagctt cgatcgcttt gtcccaatcc gccggatcgg ccatttcccc
     4081 ggagcgaatc gagtagtccg gctgtttgtc cttcatgatt ttcttgatca actctgggtt
     4141 caccgtcccc ggcgggcggc cgtatttgcc cttcacgtag tcctgggtct ctttcgtcac
     4201 gatcttgtag cgctccccgg cgaggacatt gagcaccgcc tgtacgccga cgatctggct
     4261 ggtcggcgtc agcagcggcg gatggcccag gtctttttcc acactcggag tttctttcaa
     4321 cgcggcgtcc atcaggtcca tacgtttggc ttcttttaac tgcgcgacca tgttggagat
     4381 catgccgccg ggaactttgt ggatgaacac ttccgcgctg acaccagtgt aaccggtctc
     4441 gtatttttca tatttctcct tgatctgacg ggttatcaac gacggctctt caagtacttt
     4501 cagatcgatg ccggtgtcga acggcgtgcc ggccagcgat gcgaccatgg tttcggtggc
     4561 cggatgggcg gaaccgaacg acaacggcga tagcaccgta tcgatcatgt caacgccggc
     4621 gagaaccgcc atcagatgac acatgctcga ggtgccgccc atatcgtggc aatgcagcaa
     4681 caccggcaga ttccccttcg cccggtcctt gatgcccttg atgattcggt aggcattgaa
     4741 cggcgtgaga attcctgtgg cgtctttgag cgacagccag tcggcgccga cgtcgatcag
     4801 ctgctcggcg tactccagcc atttttccag cgtgtgcacc gggctcaccg tgtaattgac
     4861 ttccacatgg gcttcgccgc cgaattcttt gaccgccttg gtcgccgtct cgatgttgcg
     4921 aaaatcattg gtagcttcga acacgcgaaa cacattgatg ccgttggcca ccgcgcgctc
     4981 gacgaacttg tagacgatct tatcggtgta cggcctatag gctaggatat gctggccgcg
     5041 cagaagcatt tgcagcttgg tcttgggcat caccgctttg atgcgccgca gccgttgcca
     5101 aggatcttcg tttaaataac gcatgcagac atcgaatgtc gcgccgcccc aaacttcgac
     5161 ggcgtagaat ccgcagttat cgatggacgc gcagagcggc aaaagatcgt cggtagtcac
     5221 ccgggtggcg agtttgcatt gctggccatc gcgcggcgtg aggtcggtga tcttgacccg
     5281 ccggctgccc gccggctcgg tgagcagtgc ggtcatgtcg acggccaatt gattcaagat
     5341 ttcttcgctc atggaaattc tcctctgttc tcgatgcgga aatatctgtc cgcgttggca
     5401 cagtttatag gccatttcag gagcgacagc aaagccagga gaatggctca tcacgaagac
     5461 atgagaaaca tacgaagcta agcggtaagc gggcggatta aaaacccgag tgacaattcc
     5521 cgattttttt tgtgtacttt gcgttctttg cagccaattt tccgtccgaa tttcttccta
     5581 ctctgtgacc tttgtgcctt ttgtggtcaa tcttcttctt gcatttacga acttattctc
     5641 ctacaaaccg gcagatgaag atcgtccgac tttatactgg cgccgataat gaatcgcatt
     5701 tcgaagatat agaagtcgag atgactgccc aaggagtcga ccaggtttcc gagctgcaac
     5761 cggcccatgg cgtcatgttt cgttcggcgc cactgagcca tcggagcggc tatcacaccg
     5821 tgccgcggcg gcaatatgtc atcacgctgt ccggccaagt cgaaatcgaa accggcgacg
     5881 gcaccgttcg ccgtttcggt gccggcgatg tcatgctcgc cgaggacacc acggggcgcg
     5941 gccacatgac tcgggtcgtc ggcgaccagc cgcgtcacta tgttttcatc ccgttgaaat
     6001 aggatacgtt catcgacccg caaccgtcgt cgctcagacg agagcgcctc tccgccgcca
     6061 tcgtaccctg ttcgaacttg atgccgtcca tgatggtggc gacgttgggc atcgccttgc
     6121 cggaaatccg ccagacgttt tcactaaccg aattacaagc cggcagtttg ttctccgtca
     6181 tgatgatcat cgccgcagtc accagcgcca tcgccggccg cttggcagat cgaatcggcc
     6241 gcaaaactgt attgataacc ggtttgagcc tgctcgcttc gggatttggc tgcgccggca
     6301 tcagcggcga tccactgctc ttcttcttgc tcctcgccgt taccggtgtc ggttacggtt
     6361 tcacgccgcc gtc
//
LOCUS       DASQKS010000444         8677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_226327, whole genome shotgun sequence.
ACCESSION   DASQKS010000444 DASQKS010000000
VERSION     DASQKS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226327"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VE170_11460"
     CDS             complement(<1..580)
                     /locus_tag="VE170_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ16098.1"
                     /translation="MFDAFIDGLFLVLQWKAFSLMLVGMGLGFCVGLLPGIGGAATLA
                     LMIPFVFKMQPAEAFAFLLGMHAVAATTGDITSVLFGVPGEAISAATVVDGHPMAKNG
                     EAGRALGAALMSSLVGAVIGAVALALAIPIVRPLVLAFGSPELFMLAVIGISCITSLS
                     GAGARGQVKGFAMGLFGLLVSTVGQERQSGSLR"
     gene            complement(869..3331)
                     /locus_tag="VE170_11465"
     CDS             complement(869..3331)
                     /locus_tag="VE170_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ16099.1"
                     /translation="MNISRPEPFRSIGSASSLLRSFPGIGSRYVWLLAVLLVGLFYLL
                     DLWTAAYFEIPISYAAALVLAVGVRGRREKIAVAASCTAMFAAEVYFIHAAAAPPTSA
                     GLTHQGFALLLVWSVTTLGLRHHSVEADRRDSERIANERLALLNTIYTSAPVGLCFVD
                     LNLRYLSMNAALAEMHDRVPEYFIGKTIREANPDLADTIEAYYRRVIESGKPILDIEV
                     KAATAARPDERHSWLASYFPVYHPSGELLGVNVAARDITARKQAEADTLFLLDLADCI
                     RFAANAEELTWVVAVALGEHVKVSRCAFLDIDTAQDRLIIRRDYHPHMPSLVGTYRLG
                     EFGPLLTEASRAGQTTTIANVALDERTRMHIESYRRVGVGAVVAVPLLRDGAVVSALA
                     MAHAEPRQWSEREVLLINMVAERTWLAVEKLRLDDALRLSGEALREADRRKDEFLATL
                     AHELRNPLSLVRNVVNLQQDASNPLPDPHWGPGIIDRQVKYLTRLTDDLFDISRITRE
                     KLVFQKEPLNLAEIVRAAVESSQPLIDQRQHELTVTMSQDSIYVDADRLRLTQVFTNL
                     LNNAAKYTPDPGHIWLNVEQAGDKVVIRVKDTGIGIAAECLPRVFELFYQVDRSFTRS
                     EGGLGLGLTLVQQLVELHGGKVEVRSEGTNRGSEFTVQLPVTAHRPDTDDLRQNGADE
                     AGLPVRSWRILVADDFPQSAETLARLLEKDGNEVQIAQDGVAAVEAAGEFLPDIILMD
                     LAMPRLDGYQAAQRIRQQPWGKPMILIALTGWGQQQDRRRTQEAGFDAHLTKPVNYDA
                     IVDLLTSLTTRVDADETRTLPN"
     gene            3501..5099
                     /locus_tag="VE170_11470"
     CDS             3501..5099
                     /locus_tag="VE170_11470"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16100.1"
                     /translation="MANKLGYYRDFREHLRALEERGKLVRIKREINKDTELMPLVRWQ
                     FRGLDERDRKAFMFEKVIDSKGKRYSMPVTVGTLAATTEIYAIGMMCEPEQIAERWNQ
                     AQLNPFDPVKVTSAPVHEEVWQGQDILNGQGLDMIPVPISTPGFDNAPYLTSANWITK
                     DPDTGIYNIGNYRSQIKSANRTGGLFTSQHMGQHWRKAKAKGQRLEACIAIGVVPSIA
                     YSATAKIPYDFDEYRLAGGLAGAPVEVVQAKTVDLLVPATAEIIIEGTIPTDVVEPEG
                     PFGEYPGYMGHRTESPFLEVTCITHRRDAIYTALMSQFPPSESSKIKHTGTEKIIYKL
                     LRHDSGNAAVVDVALHDEVSGSGQAYCVIKMRNATKAEAWRALHATAGFTGSYAKVCV
                     AVDEDIDIHDPAMINWAICYNMRPAEDVEISKGKMPGLDPSTYPPGMENEDRRAASTS
                     ALLINAIRPWPYTPVSLPKKEFMERSKQIWEELELPPLKPRMPWYGYSLGAWTKQDEE
                     EADLAVKGDYFETGKKQTGHRKKS"
     gene            complement(5222..5602)
                     /locus_tag="VE170_11475"
     CDS             complement(5222..5602)
                     /locus_tag="VE170_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ16101.1"
                     /translation="MRFFSRTPVRTFVVYPAVALTWELAFAPSQFQANLWFLPLLLWG
                     YLQYRLCGRYRVRRGGGGPGVETPPERLVTTGLYRYTRNPMYLGHLIFLIGLTLPLKS
                     WLAASITSPRQYGFTAVLSAMKKS"
     gene            5618..6373
                     /locus_tag="VE170_11480"
     CDS             5618..6373
                     /locus_tag="VE170_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYJ16102.1"
                     /translation="MSFDLTHIFVGVALLFAAFIKGATGLGFPLIATPMVALLLDIRV
                     AVTILILPNLLMDITQVFRDGFPNAVLQRFKSMIGLTVIGVFLGTMVLVLLPLWVLNL
                     CLGIMVIVFVVSNLLKFDFVISPATEKTLSPIVGLLSGFLNGMTNAAGPLLAIYFYSL
                     KLEKRNFVKSVATIFMITKAAQLVAVSTWNLFNWNTLTLSLQVVLFTLIGFFAGLKIQ
                     DRVNQTTFSRGLMVLLAIIGVVLITRALTQALP"
     gene            complement(6525..7247)
                     /locus_tag="VE170_11485"
     CDS             complement(6525..7247)
                     /locus_tag="VE170_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HYJ16103.1"
                     /translation="MATWDDILTPRDKEVFALSGFGKKAGLGQRPALLIIDVNYNFTG
                     DKPEPILESVKRFRTSCGEEGWDGVHAIGELLGEARKKHMPTFYTTAEENRANSTVLV
                     GGWAAKSNRSTEDMTEQWEKANQIVDEIAPQPGDIIVRKQKPSAFFGTALMSMLTEVH
                     ADSLLVCGTTTSGCVRASVIDAFSYNLKVSVVEECVFDRGQASHKINLFDMAMKYADV
                     IPLKEAIDYVRALPNNLYAPAL"
     gene            7369..7956
                     /locus_tag="VE170_11490"
     CDS             7369..7956
                     /locus_tag="VE170_11490"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ16104.1"
                     /translation="MLRQATRDSSLNLKRVREKLRLVLAMLLWSSCGSPIAIWTDGEV
                     PFVRSTPEVIERMLELARVKPGDVIYDIGSGDGAIIIHAAKKYGVKGVGIEIDQDLVT
                     RARNNAYREKVEHLVEFRAQDAFTVDISPATVVTLYMLPEFNAKLRPILDRQLKPGTR
                     VVSHDYPVEGWAPDRVELVHGDFVHNHTVLLFEIR"
     gene            complement(8010..>8677)
                     /locus_tag="VE170_11495"
     CDS             complement(8010..>8677)
                     /locus_tag="VE170_11495"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HYJ16105.1"
                     /translation="QVQHLVFSGGVSEHVYDRDAKSYGDIGPVLGKNMRDYLKKLPKD
                     VLREPIEGIRATVIGAGEYTIQASGNTSYVSNEKALPVHGLKVIQAPVRDGESVADAI
                     KQSLRKFDLSCFTSGLALSLTVSGVPDYQSVRRIAEGVAQILKEADDTTCPLYLTLDL
                     DIAKSLGAILREELKVSRDIIAIDGIEVGDLDYIDIGECLGITEVIPVTVKSLMFPTT
                     VSD"
BASE COUNT         1992 a         2527 c         2399 g         1759 t
ORIGIN      
        1 aacggaggga cccggactgg cgctcttggc caacggtcga caccagcaga ccaaacagac
       61 ccatggcgaa acccttgacc tgtccgcgtg cgccggcgcc gctgagcgag gtgatgcagg
      121 atatgccgat caccgccagc atgaatagct ccggcgaacc aaaggcgagc accagcggac
      181 ggacaatcgg aatcgccaac gccaatgcca ctgcgccgat caccgcgccg accagcgagc
      241 tcatcaacgc ggcgccgagg gcgcgtccag cctcgccgtt tttcgccatc ggatgaccgt
      301 cgaccaccgt ggcggcggat atcgcttcac ccggcacgcc gaaaagcact gatgtgatat
      361 cgccggtagt cgcggcgacg gcatgcatgc caagcaggaa ggcgaacgcc tcggcgggct
      421 gcattttgaa gacgaacggg atcatcaaag cgagagtcgc cgctccgcca atgccaggaa
      481 gcagaccgac acagaagccg aggcccatac cgaccagcat caagctgaat gctttccatt
      541 gtagcaccag gaaaagaccg tcgatgaagg cgtcgaacat cgtctgactg cctctaccgc
      601 ggcgactgaa ccgggttaca aaacctcaga cgtaattggg agacaaacac cggcgcgact
      661 atacgggagc ggcaatttta ctgtcaagca cagattggtg ctaagcggcg aaatgtcgat
      721 taggatcaac ctcagaccaa gggtcagtaa cttttctgct ggatagcgtc acagtaactt
      781 accgtgcctt gggtacagct cgacacttaa gtctggtgga taccggcgct gatcttgttg
      841 gaaaatttcc gccaccgaca aatattgctt aattcggcag agtgcgagtt tcgtcggcgt
      901 cgacgcgcgt agtgagactt gtcagcaaat cgacgatggc gtcgtaattg accggtttgg
      961 ttagatgggc gtcgaagccg gcttcctggg tacgacgccg gtcctgctgt tgtccccatc
     1021 cggtaagggc gatgagaatc atcggctttc cccaaggctg ttggcgtatc cgttgtgcgg
     1081 cttggtatcc gtcgagtctc ggcatggcca ggtccatcaa aataatatcc ggcaagaatt
     1141 ccccagcagc ttccaccgct gcgacgccgt cctgggcgat ttgcacttcg ttgccgtctt
     1201 tttccagcag gcgcgccaag gtttcggcgg attgggggaa atcatccgcc accaggatgc
     1261 gccagctccg aaccggcaag ccagcttcgt ccgcgccatt ttgtcgaagg tcgtcggtgt
     1321 ccggccgatg cgcggtgacc ggtaattgca cggtgaattc gctgccgcgg ttcgtgccct
     1381 cgctgcgcac ctcaactttg ccgccgtgca gttcgaccag ttgttgcacc agtgtcaaac
     1441 ccagacccag gccaccctcg ctgcgtgtaa acgagcgatc cacctggtaa aacaactcga
     1501 agacacgggg caagcattct gcagcaatac cgattccagt atctttgact cggatgacga
     1561 ccttatcacc cgcctgctcg acattgagcc aaatatggcc tggatccgga gtgtacttgg
     1621 cggcattatt caaaagattc gtgaagacct gggtaagtcg aagccgatcg gcgtcgacgt
     1681 agatcgaatc ctgcgacatg gtcacggtca actcatgttg ccgttgatcg atcaacggct
     1741 ggctcgattc caccgccgcg cggacaatct cggcgagatt gagcggttcc ttttggaaaa
     1801 ccaacttttc ccgagtgatg cggctgatat cgaagagatc gtcggtcaag cgcgtcagat
     1861 atttcacctg acgatcgata atacccggac cccaatgcgg atcgggtaat ggattactgg
     1921 cgtcttgttg gagattgacg acgttgcgaa ccagcgacaa aggattgcgt aattcgtggg
     1981 cgagagtggc taagaactcg tctttgcgcc ggtcggcctc acgcagagcc tcgccgctca
     2041 aacgcagggc gtcgtcgaga cgcaattttt caaccgccag ccaggtgcgt tcggcgacca
     2101 tattaatgag caagacttcc cgttcgctcc actgacgcgg ctcggcgtgc gccattgcca
     2161 atgcggatac caccgcgcca tcgcgcagca ggggcaccgc gaccacggca cccacgccga
     2221 cccggcggta agactcgata tgcattcttg tccgctcgtc cagggcgacg tttgcgatcg
     2281 ttgtggtttg accggcacgg ctggcctcgg tcaacaacgg tccaaactcg cccagccgat
     2341 aggtaccgac taacgacggc atatgcgggt gataatcgcg ccgaatgatc aaccgatcct
     2401 gggccgtatc gatgtcaaga aacgcgcagc ggctgacttt gacatgttcg cccaaggcca
     2461 ccgcaaccac ccaggtgagc tcctcggcgt tggccgcgaa gcgtatgcag tcggcgagat
     2521 ccagcagaaa aagcgtatcg gcttcggcct gtttccgcgc tgtgatatcg cgcgccgcga
     2581 cgttgactcc gagtaattcg ccgctcgggt ggtacaccgg gaaatagctg gctagccaag
     2641 aatggcgctc gtcggggcgc gccgccgtag cagctttgac ttcgatatcc aggatcggtt
     2701 taccgctctc gatcacccgg cggtaataag cttcgatggt atccgccagg tccggattgg
     2761 cctcccgaat ggttttaccg ataaaatact ccggcacccg atcatgcatc tcggctaaag
     2821 cggcgttcat cgataaatag cggagattga gatcgacaaa acacaaaccc accggtgccg
     2881 aagtatagat ggtgttgagc agagcgagcc gttcattggc gatgcgctcg ctatcgcgcc
     2941 ggtccgcttc gaccgagtga tgacgcaacc ctaaagttgt cacgctccag accaacaata
     3001 gagcaaaccc ttgatgcgtc aatcctgccg atgtcggcgg agccgcggcg gcatgaatga
     3061 aataaacctc cgccgcgaac atcgccgtgc acgacgccgc cacggcaatt ttctcccgcc
     3121 tgccgcgtac ccctacagcc aagaccaagg cggcggcgta gctgatggga atctcgaagt
     3181 acgccgccgt ccacaggtca agtagataaa acaggccgac taacaagact gcgagcagcc
     3241 aaacgtagcg cgagccgata ccgggaaagc tgcgtagcag tgacgaagca gagccgatac
     3301 tgcgaaacgg ttcagggcgc gaaatattca tcacttgtag tcgtaccgca tagaactcta
     3361 gaacagtgcc gcagtgtatc agacacgctc tagatcaaac aagcatcaaa gcgcccgccc
     3421 caccggtttg cttggggacg cttgacaacc tcccgagccc atacatagat gacggaagca
     3481 tctatgatag ggagaaaatg atggccaaca agttaggtta ttaccgagat tttcgcgagc
     3541 atttaagagc cctcgaagag cgtggcaaat tggtccgtat caagcgcgaa attaacaaag
     3601 acaccgaact gatgccgctg gtgcgctggc agtttcgcgg gctcgatgaa cgcgaccgca
     3661 aggcgttcat gttcgagaaa gtcatcgatt ccaagggtaa acgatactcg atgccggtaa
     3721 ccgtgggaac cttggcggct accacggaaa tctacgccat cggcatgatg tgcgaacccg
     3781 agcagatcgc cgagcgctgg aatcaagcac agttgaaccc cttcgacccc gtcaaggtaa
     3841 cctcagcgcc cgtccatgag gaggtctggc aaggtcaaga tattctcaac ggccagggcc
     3901 tcgacatgat cccggtgccg atctcgaccc caggcttcga taatgcgcca tacttaactt
     3961 cggccaactg gatcaccaag gatcccgata ccggcatcta taacatcggc aactatcgca
     4021 gccagatcaa gtcggccaac cgcaccggcg gcctgttcac tagccaacat atgggacagc
     4081 actggcgcaa ggcgaaagcc aaggggcagc ggctggaagc ctgcattgcc atcggtgtcg
     4141 tgccgagcat cgcctattcg gcgactgcca agattcccta cgacttcgac gaataccgcc
     4201 ttgctggcgg cctggccggc gcgcccgtcg aggtcgtgca agcgaaaacc gtcgacctac
     4261 tcgtgcctgc caccgcggaa atcatcatcg aagggacgat tcccaccgac gtcgtcgagc
     4321 ccgaaggccc gtttggcgag tatccgggct acatggggca tcgcaccgaa tcgccatttc
     4381 ttgaagtcac ctgtatcacg caccggcgcg acgcgattta caccgcgctg atgagccagt
     4441 tcccgcccag cgagtcgagt aagatcaagc acaccggcac ggaaaaaatt atttataaac
     4501 tattgcgcca tgacagcggc aatgcggcgg tggttgacgt cgcgctccat gatgaagtca
     4561 gtggcagcgg ccaagcctat tgcgtgatca aaatgcgcaa cgcgaccaag gccgaagcct
     4621 ggcgcgcgct ccatgccacc gctggattca ccggcagtta cgccaaagtc tgcgtcgccg
     4681 tggacgaaga catcgacatt catgatccgg cgatgatcaa ttgggcgatt tgctacaaca
     4741 tgcgccccgc ggaggatgtc gagatctcca aaggtaaaat gcccgggctc gatccgtcga
     4801 cctacccgcc gggaatggaa aatgaagacc ggcgcgccgc atcaacatcg gcattattga
     4861 tcaacgccat tcgtccttgg ccctatacgc cggtgtcgct gcccaagaaa gagtttatgg
     4921 agcggtccaa acagatctgg gaagagctcg agctcccgcc gctcaagccg cgcatgccct
     4981 ggtacggtta cagcttgggc gcttggacta agcaagacga agaagaagcc gatttagcgg
     5041 tcaaaggcga ctacttcgaa accggcaaaa agcagaccgg acacaggaag aaatcgtaag
     5101 tgcggatcag gaaatggcgg aagaataact tcgtccatca ttcttctacc ttagaaaaga
     5161 gctggcaccc aacgtttcac cgccgccata taattcacat aaggttggcc gaggatttga
     5221 attagctttt tttcatcgcc gacaacacgg cggtgaaacc atattgccgt ggcgacgtga
     5281 tcgacgctgc gagccaggat tttaagggca gggtaaggcc gatgagaaag atcagatggc
     5341 ccaagtacat cgggtttcgc gtatagcgat agagaccggt ggtaaccaac cgctccggcg
     5401 gcgtttccac tcccggaccg ccgcccccac gcctcacccg gtagcggccg cacagtcgat
     5461 attgcaaata accccaaagc aacagtggca ggaaccagag attggcttga aattggcttg
     5521 gcgcaaacgc cagctcccac gttaacgcga cggccggata gacaacaaag gttcgcaccg
     5581 gtgtgcggct aaagaatctc atcgttgccc caacagtatg tccttcgacc taacccatat
     5641 tttcgtcggc gtcgctttgc tatttgctgc cttcatcaaa ggcgccaccg gtttgggctt
     5701 tccgctgatt gcgacgccga tggtcgcgct actcttggac attcgcgttg ccgtgacaat
     5761 acttatcctg cccaatcttt tgatggacat cacccaggtg tttcgcgacg gcttcccgaa
     5821 cgccgtcctg cagcgtttca aatccatgat cgggctgacc gtcatcggag tttttcttgg
     5881 cacgatggtc ctagtgctgc tgccgctatg ggtgctcaat ttgtgtctcg gcatcatggt
     5941 catcgtcttc gtagtgtcca acctgctcaa gttcgacttc gtcatatcac cggcgacgga
     6001 aaaaaccctg tcgccgattg tcgggctcct gagcggattc ttaaacggca tgaccaatgc
     6061 agccggaccg ctcttggcaa tttacttcta tagcctaaaa ttagaaaagc gtaacttcgt
     6121 caaatccgtc gcgacgatct ttatgatcac caaggcggcg cagttggtcg cggtgtcgac
     6181 ctggaatctg ttcaattgga ataccctcac gctatcgctg caagtggtgc tgtttacctt
     6241 gattggattt ttcgccggac tgaagatcca ggatcgggtg aatcaaacga cctttagtcg
     6301 gggcttgatg gtgctgttag cgatcatcgg cgtagttcta atcacgcgcg cgctgaccca
     6361 ggcactacct tgattcctgg ctagaaaagt aagctggtta ggcaaaagct agcctatcga
     6421 ttggatggca gcacaaaggt cagagggatg cagggggttg acagctccgg agtgaaaggc
     6481 tgccgcttgt tcaggatgtc aaacctcggt cgcggctagt ttgctcacag cgccggcgcg
     6541 taaagattat tcggcaaagc gcgcacgtaa tcgatcgcct ctttgagcgg gatcacgtcg
     6601 gcatacttca tcgccatgtc gaacagattg atcttgtgcg aagcttgacc gcggtcaaaa
     6661 acacattctt cgaccaccga aacttttaga ttataggaaa acgcatcgat gacgctcgcg
     6721 cgcacgcagc cgctcgtggt ggtgccgcaa accagcaagc tgtcggcatg gacctcggtc
     6781 agcatgctca tgagcgccgt gccaaaaaaa gcgctgggct tttgcttgcg cacgatgatg
     6841 tcgccgggct ggggcgcgat ctcgtcgacg atctgattgg ctttctccca ctgctcggtc
     6901 atatcttccg tggatcgatt gctcttggct gcccacccgc cgaccagcac ggtggaattg
     6961 gcgcgattct cctcggcggt ggtataaaac gtcggcatat gctttttgcg agcttccccg
     7021 agcaactcgc cgatggcatg gacgccatcc caaccttcct cgccgcaact ggtgcgaaag
     7081 cgcttgaccg attcgagaat cggctccggc ttgtcgccgg taaaattata attgacgtcg
     7141 atgatcaaca gcgccggtcg ttgaccgagg ccggctttct tgccgaaacc ggaaagcgca
     7201 aacacttctt tatcgcgcgg cgtcagaata tcgtcccagg tcgccatgag aaatcctccc
     7261 tatcggcaag ttagggcaat gttcataaca aaccgccggc gatgtcaaga tgatattgct
     7321 gccggcgcat tgaccgcgac agggaccgcg cctataacag gagagccaat gctgcgacaa
     7381 gccacaagag atagctcatt aaacctgaag cgggtccgcg agaagctgag attagtgttg
     7441 gcaatgctgc tgtggtccag ctgcggttcg ccgatagcca tctggaccga cggcgaggtc
     7501 ccgtttgtcc gctcgacgcc ggaagtgatc gagcgcatgc tcgaattggc gcgggtcaaa
     7561 cccggcgatg tcatctacga tatcggcagc ggcgacggcg ccatcatcat ccatgccgcc
     7621 aagaaatatg gcgtcaaagg cgtcggcata gaaatcgacc aggatttagt cacgcgggcg
     7681 cgcaacaacg cttatcggga aaaagtcgaa catctcgtcg agttccgcgc ccaggatgcc
     7741 ttcaccgtcg acatctcgcc ggccaccgtc gtgactttgt acatgctgcc tgagttcaac
     7801 gccaagttaa ggccgatcct ggaccgccag ctcaaacccg gcacccgcgt cgtctcccac
     7861 gattatcccg tggaaggatg ggcgccagac cgcgtcgagc tggttcacgg cgacttcgtg
     7921 cataaccata cggtgctgct cttcgagatc cgctaaacgc aaaaggcccc gcactggcgg
     7981 ggccctttga ttagtccctc gaaaaaatgt taatcgctca ccgtcgtcgg aaacatcagc
     8041 gatttaaccg tcaccggaat cacctccgtg atacctaagc attcgccgat atcgatataa
     8101 tccagatcgc cgacttcgat accgtcaatc gcgataatgt cccgagaaac tttgagctcc
     8161 tccctgagaa tggcgccgag tgacttggca atgtccaagt ccaaagtcag gtagagcgga
     8221 caagtggtat catcggcttc tttgagaatc tgcgccacgc cttcggcaat gcgccggacc
     8281 gattggtagt cgggcacgcc gcttactgtc aatgacaagg ccaaacctga cgtgaagcag
     8341 ctcaggtcga actttctgag cgactgtttg atcgcatcgg ccaccgattc gccgtcgcgg
     8401 accggcgctt ggataacttt caatccatgc accggcaagg ctttctcgtt ggagacataa
     8461 ctggtattgc cgctcgcttg aatggtgtac tcgccggcgc cgatgaccgt ggcgcgaatc
     8521 ccttcgatgg gctcgcgcag gacatccttc ggcagctttt tcaaatagtc gcgcatgttc
     8581 ttgccgagca ccggcccgat atcgccatag gatttggcat cgcggtcgta aacatgctcg
     8641 gaaacgccgc cggagaacac cagatgctgc acttgtt
//
LOCUS       DASQKS010000445         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5272882, whole genome shotgun sequence.
ACCESSION   DASQKS010000445 DASQKS010000000
VERSION     DASQKS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5272882"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VE170_11500"
     CDS             complement(<1..260)
                     /locus_tag="VE170_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16106.1"
                     /translation="MTKSKINHGNVAQSSYQQLLDDLPRTGPGTKGGAMLRRYWHPVC
                     LSANLQDIPIAVRMLSEDLVVFRDGKGRPGLLGIRCPHRLASL"
     gene            402..1418
                     /locus_tag="VE170_11505"
     CDS             402..1418
                     /locus_tag="VE170_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ16107.1"
                     /translation="MTIGITTLARAVLLLVLPITSAQAGPTADGALKKGFIQCGVNTG
                     LAGFAQPDSNGEWRGIDVDLCRAVAAALFSDAKKNRYTPLTAQQRFTALQSGEVDILS
                     RNTTWTFARDTGLGLNFVGVNFYDGQGFMVTKKRNVKSAKQLNGAAICVQPGTTTELN
                     MADYFRAHKMKFKPVVIEKLEEVLNAYFTGRCDVFTTDVSGLIAVRAARATNPADHVI
                     LPEVISKEPFGPVVRHGDDHWHDLVRWTLFAMIEAEELGLTSKNIDQQAQNSNPVVQR
                     FVGATGDFGKMLNLDNRWAYNIVKQVGNYGESFERHLTPLGLDRGLNKLWNKGGIMYA
                     PPLR"
     gene            1453..2439
                     /locus_tag="VE170_11510"
     CDS             1453..2439
                     /locus_tag="VE170_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746028.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ16108.1"
                     /translation="MARRLDSRQRAALYQILLLAAGCLVGWYFIRNAVDNLLARRIAS
                     GFVFLFREAGFEIGETTLVSFSAADSYLKALTVGLLNTFRVALFAVGSSTIIGVAVGL
                     ARLSPNWLLAKLASGYVELLRNVPLVVQLFFWYAVITESLPAPAQALTPLPGIYLSNR
                     GLAVPTIASLLPFEVEYPSLSGFNFSGGVALSPEFAALLLGLALYTAAFIGEIVRAGI
                     LAVDRGQYEAAQSQGLSQAQLMRLIILPQALRVVVPPVTSQYLNCVKNSSLAVAIGYP
                     DLVSIANTTINQTGQAIEGIAIIMLVYLSISLAIAGLMNLYNRRIALRGART"
     gene            2436..>3505
                     /locus_tag="VE170_11515"
     CDS             2436..>3505
                     /locus_tag="VE170_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HYJ16109.1"
                     /translation="MNPGVGFPAAMWHWTRTRLFSSWPSSIISLTIIYLAVKLLPGLI
                     DWALVRAVWSPHNAAACRDAATGGACWAFVAEKHRFFLFGTYPADEHWRPALVIASLL
                     ALYAVSACQLVRRLNLFLLWLVALVFIGILMWGGVFGLAYVENERWGGLTLTLLLSTF
                     GIALAFPLSILLALGRRSEMPLVRWFSIGYIELIRGVPLISVLFMASVMLPLFLPQGV
                     SIDKLLRAQLAMIFFAAAYLAEVIRGGFQAIPPQQEEAALATGLGYWQRVRYVTLPQA
                     LHVAIPPLVNTFIGFFKDTSLVVIIGLFDFLTTIKVSLSEPAWSGFGIEAYLFAALGY
                     FLFCYPMSRYSQALERQTAHGTG"
BASE COUNT          643 a         1127 c          958 g          777 t
ORIGIN      
        1 agcgaagcca ggcgatgggg gcaacgaatg cctaacagac cgggccgtcc cttgccatcg
       61 cggaaaacca ccagatcctc actcagcatc cgcaccgcga tcgggatgtc ttgcagattg
      121 gccgacaaac agaccgggtg ccaatagcgc cgcagcatcg ccccgccctt ggtgccgggg
      181 ccggtgcgcg gcaagtcgtc gagcaattgc tggtaggaac tctgcgcgac gttgccgtgg
      241 ttgatcttgc tcttcgtcat tgccgtcctc caccatgccg aatattgctt ttcattccaa
      301 agaatatcta taaacgcaga ctaaccgctg tcaacaattg gccgagtgta ataagttcgg
      361 cgcacagact caaaccaccg ccatcatgag gaaggacgac catgaccatc ggaattacca
      421 ctttagcgag ggcagtgctg ctccttgtcc tgcccatcac cagcgcccag gccggcccca
      481 ccgccgatgg cgcactcaag aagggcttta ttcagtgcgg cgtcaacacc ggcctcgccg
      541 gcttcgccca gccggatagc aacggtgaat ggcgcggtat cgatgtcgac ttatgccgtg
      601 ccgtggccgc ggcgctgttc agcgacgcta agaaaaaccg ttacacgccg ctcaccgccc
      661 agcagcgctt caccgccttg cagtccggcg aagtcgatat tctctcgcgc aataccacct
      721 ggaccttcgc ccgcgacacc ggcctcggcc tcaattttgt cggggtcaat ttctacgacg
      781 gccaaggctt catggtgacg aaaaaacgca acgtcaaaag cgccaagcaa ctgaacggtg
      841 ccgccatctg cgtgcagccc ggcactacca ccgaacttaa tatggccgat tacttccgcg
      901 cccataagat gaagttcaaa ccggtagtca tcgaaaagct cgaagaggtc ttgaacgcct
      961 atttcaccgg ccgttgcgat gtctttacca ccgacgtctc aggattgatc gccgtgcgcg
     1021 ccgcgcgggc gaccaacccg gccgaccatg tgatcttgcc ggaggttatt tccaaagaac
     1081 ccttcggtcc ggtggtgcgc catggcgacg accattggca tgacctcgtc aggtggaccc
     1141 tgttcgccat gatcgaagcc gaagagctcg gcctgacttc gaaaaacatc gatcagcaag
     1201 cccaaaactc caaccccgtg gtgcagcgct tcgtcggcgc cactggcgac ttcggcaaga
     1261 tgctcaacct cgacaaccgc tgggcttaca acatcgttaa acaggtcggt aattacggcg
     1321 aaagcttcga gcgccatctg accccgctgg gcctcgaccg cggcctcaac aagctatgga
     1381 acaaaggcgg catcatgtac gcgccgccat tgcgctgagc gcttcgatcg attgcgagag
     1441 cggcaatttc caatggcccg gcgactcgac agccgccagc gcgcggcgct ctatcaaatc
     1501 ctcttgctgg cggccggctg cctcgttggc tggtatttca ttcgcaatgc ggtggacaat
     1561 ctgctggcgc gccgcatcgc cagtggcttt gtttttctgt ttcgcgaggc cggtttcgag
     1621 atcggcgaga ccactcttgt ttccttcagc gctgccgata gttacctgaa ggcgctcact
     1681 gtcggcctgc tcaacacttt ccgggtcgcg ctgttcgccg tgggttcatc gacgattatc
     1741 ggtgtcgccg tcgggttagc gcgtttatcg cctaactggc tcttggccaa gctcgcgtcc
     1801 ggatatgtcg aactgctgcg caacgttccc ctggtcgtgc aactattttt ttggtacgcc
     1861 gtaatcacag aaagcctgcc ggcaccggcc caggcgttga ctcctttgcc gggaatttat
     1921 ctcagcaacc gcggcctggc agtgccgacg atcgccagct tgctcccatt cgaggtcgaa
     1981 taccctagcc tctcgggatt caatttttcc ggcggtgtag cgctgtcgcc ggaatttgcc
     2041 gcgctgctgc tcggcctggc gctttacacc gcggcattta tcggcgaaat cgtccgcgcc
     2101 ggcatcctcg ccgtcgaccg cggccagtac gaggcggcgc agtcccaggg attgagccaa
     2161 gcccagctca tgcgcttgat catccttccg caagccctgc gcgtggtcgt gccgccggtc
     2221 accagccaat acttgaactg cgtcaaaaac agctccctgg ccgtggccat cggctacccc
     2281 gacctggtgt ccatcgccaa cacgaccatc aaccaaaccg gccaagccat cgaaggcatc
     2341 gccataatca tgctggttta cttgagcatc agcctggcta tcgccgggct catgaacctc
     2401 tacaatcggc gcatcgccct gcgcggagcg cgcacatgaa ccccggcgta ggattccctg
     2461 ctgccatgtg gcactggaca aggacgcgat tgtttagcag ttggccgtct tcgatcattt
     2521 ccctgacgat tatttattta gccgtaaagt tgctgccagg actgatcgat tgggcgttgg
     2581 tccgcgccgt gtggtcgccg cacaacgccg cagcctgccg cgatgccgcc accggcggcg
     2641 cctgctgggc tttcgtcgcg gaaaagcacc gatttttttt attcggcaca tatcccgcag
     2701 acgaacactg gcgacccgcc ttggtcattg ccagcttgct cgcgctttac gccgtcagtg
     2761 catgccagct ggtgcgccgg ttgaatctgt ttttgctctg gctggttgca ctcgtattca
     2821 tcggtatact tatgtggggc ggggttttcg gcctcgccta cgttgaaaac gagcgctggg
     2881 gcggcctgac tctgactctc ctgctcagca ccttcggcat cgccttggcg tttcccctgt
     2941 cgattctctt ggccctcgga cggcgctcgg aaatgccctt ggtccgctgg ttttccattg
     3001 gctatatcga attgattcgc ggcgtaccgt taatctcggt gctgttcatg gcctcggtca
     3061 tgctgccgct gttcttgcct cagggtgtgt ccatcgacaa gctcctgcgc gcccagctgg
     3121 cgatgatctt ttttgccgcg gcctatttgg ctgaggtgat tcgcggcggc ttccaggcga
     3181 tcccaccgca gcaggaggaa gccgccctcg ccaccggcct cggctactgg caacgggtcc
     3241 gttacgtcac cctgccgcaa gccctccacg tcgccatccc gccattggtc aataccttca
     3301 tcggtttttt taaggatact tcgctggtgg ttatcatcgg tctgttcgac tttctcacga
     3361 cgatcaaagt atccctcagc gaaccggcct ggagcggctt cggcatcgag gcgtatttgt
     3421 tcgccgccct gggatatttc cttttctgtt atccgatgtc gcgctatagc caggcgctgg
     3481 agcgccaaac cgcccatggc accgg
//
LOCUS       DASQKS010000446         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_227040, whole genome shotgun sequence.
ACCESSION   DASQKS010000446 DASQKS010000000
VERSION     DASQKS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227040"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VE170_11520"
     CDS             <1..1431
                     /locus_tag="VE170_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYJ16110.1"
                     /translation="DVIITNHQAVAGQHLNNIVIYTPYIRDGELLCFAMVRAHWIDVG
                     GGSTGFGSSNTRDVYEEGLQVRSVKIYKRGQPNAEVLRLIEDNIRFPESSLGDLRAQI
                     ACCRTGEERLQQICRKYGGARFQAAVQAIWAQTDKLVRESVRGIPDGVYEAASFLDDD
                     GRVMNKTIPLKIKVVVRGDELTIDYSEVADQVPGFINCGESGGMAAARVAFKALTSPQ
                     REVNEGSFLALKVILPPGKLLSARRPAPIGGWSLSLPTVLDTIFCALAPALPNLVPAA
                     HKGDMGGYAIFGMNSKTGRRYVCQNIIGGGWGGRPFEDGVSSAVSMCQGDVKNTPIEL
                     QELYYPLLYECHAFRTDSGGAGKFRGGNGVEVRVKPLENCFLSRNTDRIKCPPWGLLG
                     GAAAMVNETRIKRGGKDEQLPGKFSQMQIHRGETVTFLTAGGGGYGAPGERKAAAVAR
                     DVELGYSSPQNARKNYPQAFPAEAKT"
     gene            1459..2517
                     /locus_tag="VE170_11525"
     CDS             1459..2517
                     /locus_tag="VE170_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16111.1"
                     /translation="MKKLIRYAPMMFATLLYCADGWAQAKKAVSLAELAAYTGADREQ
                     RLIAGAKAEGKVVWYTSLAGSSYKDLAKGFESKYGIKIEPYRATSTDIMTKVTAEAQA
                     KQVIADVIETTLPTLRFLRESKMLIPYTSVHLAKYPAHAKENATKGLVYWGIDRETYM
                     GVGYNTNVIPAAAIPKNYGDLLKPELKGKIGFVSNETGTRTLGSILNVKGEEFVKKLR
                     GQDITLQNVSGRAMADLVLSGEVPLSPTIFRDHALEMKAKGGPIDWAAMEVVPTNAGG
                     VVIVAQAPHSHAAVLLTDYLLGPEAAKILGDLEYGSVFKPVSYKLWYPESGMSTAQYD
                     KAAERWEKLLREIGKKQS"
     gene            complement(2534..>2901)
                     /locus_tag="VE170_11530"
     CDS             complement(2534..>2901)
                     /locus_tag="VE170_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16112.1"
                     /translation="LSGRSCKLQKSATDAFPTFRPFTKSWINKVSEAGRRLATVLLAP
                     AVFGRPMVAAPGVPPARVKILRDAYHRSLKDPGLLEELKKRRWVVDAIGGEEMAALAR
                     EVIAQPPEIIERMKKLLGS"
BASE COUNT          608 a          833 c          900 g          560 t
ORIGIN      
        1 gacgtgatca tcaccaacca ccaggcggtg gcgggtcagc atctgaacaa catcgtcatc
       61 tatacgccct atatccgcga cggcgagctg ttgtgcttcg ccatggtacg cgcccattgg
      121 atcgacgtcg gcggcggcag caccggtttc gggtcgagca atacgcgcga tgtttatgaa
      181 gaggggctgc aagtacgctc ggtgaaaatt tacaagcgag gccagccaaa cgcggaagtg
      241 ctgcgcttga tcgaagacaa tattcgcttc cccgagtcat cgcttggcga cttgcgggcg
      301 cagatcgcgt gctgccgcac cggcgaggag cgtttgcaac aaatttgccg caaatacggc
      361 ggcgcgcgct ttcaagcggc ggtgcaagcg atctgggctc agaccgataa gctggtgcgc
      421 gagtcggtgc gggggattcc cgacggcgtc tacgaagcgg cgtcgtttct cgacgacgac
      481 ggccgggtca tgaacaagac tattccgctc aagatcaaag tcgtcgtgcg cggcgatgag
      541 ctgaccatcg attattctga agttgccgat caagtgccgg gctttatcaa ctgcggcgaa
      601 tccggcggca tggccgccgc gagggtcgct ttcaaggcgc tgacgtcgcc gcaacgtgaa
      661 gtgaacgaag gatcctttct cgcgttgaaa gtgatcctgc caccgggaaa attgctcagt
      721 gcgcgccggc cggcgcccat cggcggctgg agtctgtcgt tgccgaccgt gctcgatacg
      781 atcttttgcg ccctggcgcc ggccttgccg aatcttgtgc cggcggcgca taaaggcgac
      841 atgggcggct atgcgatttt cggcatgaac tccaaaaccg gccggcgtta cgtctgccag
      901 aatattatcg gcggcggctg gggcggacgg cccttcgaag acggcgtttc gtcggcggtg
      961 tcgatgtgtc aaggcgatgt caaaaacacg cccatcgaat tacaggaact ttactatcca
     1021 ttgctttatg agtgccatgc ttttcgcacc gacagcggcg gcgccggcaa gtttcgcggc
     1081 ggcaacggcg tcgaagtgcg ggtcaagccg ctggagaatt gtttcctgag ccgcaacacc
     1141 gaccgcatca agtgcccgcc ctggggcttg ctgggcggcg ccgcggcgat ggtcaacgag
     1201 acgcgcatca agcgcggcgg caaagacgaa cagttgcccg gtaagtttag ccagatgcaa
     1261 attcatcgcg gcgaaacggt aacctttttg accgccggcg gtggcggcta cggcgccccc
     1321 ggcgaacgca aagcggcggc ggtggcgcgc gacgtcgaac tcggttattc gtcgccacag
     1381 aacgcccgta agaattatcc gcaggccttt ccagccgagg cgaaaacctg accaacaatt
     1441 gtcgaatcgg aggcggtcat gaaaaaactc attcgatatg cgccgatgat gtttgcgact
     1501 ctgttgtatt gcgctgacgg ttgggcgcag gcgaagaaag ccgtttccct cgccgagctc
     1561 gccgcttaca ccggcgccga ccgcgagcag cggctgatcg ccggggcaaa agcggaaggc
     1621 aaggtggttt ggtacacgtc gctcgccggc agctcataca aggatctcgc caaaggattc
     1681 gagagcaaat acggcatcaa gatcgagccg taccgcgcca ccagcaccga tatcatgacc
     1741 aaggtcacgg ccgaggcgca ggccaagcag gtcatcgccg acgtgatcga gacgacgctg
     1801 ccgaccctgc gttttctgcg cgagagcaag atgctgattc cttacacttc ggtgcatctc
     1861 gccaaatatc ccgcccatgc caaggaaaat gccacaaaag gattggtcta ctggggcatc
     1921 gatcgcgaga cctacatggg cgtagggtac aataccaacg tcatccccgc cgcggcgata
     1981 ccgaaaaact atggcgatct attgaagccg gagctgaaag gtaagatcgg cttcgtctcg
     2041 aacgagaccg gcacacgcac cctcggctcg atactcaacg tcaaaggcga agagttcgtc
     2101 aagaagctcc ggggccagga tatcactctg caaaatgtct cgggccgggc gatggccgat
     2161 ttggtgttgt caggcgaagt accgctctcg ccgacgattt tccgcgatca cgcgctggag
     2221 atgaaagcca aaggcggtcc catcgattgg gcggcgatgg aagtagtgcc gaccaatgcc
     2281 ggcggcgtgg ttattgtcgc ccaggcgccg cattcccatg ctgcggtttt gctcaccgac
     2341 tatctcctcg gacccgaagc ggcgaaaatt ctcggcgacc tggagtatgg cagcgtgttc
     2401 aagccggtct cctacaaact ctggtatccc gaatcgggaa tgtcgacggc gcagtatgac
     2461 aaggccgccg agcgctggga gaagttgctg cgggagatcg gcaaaaagca gtcgtaatag
     2521 ttgccgggtt atatcaactt cctaacaatt ttttcatgcg ttcgatgatc tccggcggct
     2581 gggcaataac ttctcgcgcc agagccgcca tctcctcgcc gccgatggca tcgacgaccc
     2641 agcggcgctt tttcagctcc tccagcagcc cagggtcttt gagcgatcga tggtaggcgt
     2701 cgcgcaggat ttttactctc gcaggcggca cgccgggggc ggctaccatg ggtcggccga
     2761 agacggcggg cgcgagcagg accgtggcta gacggcgacc ggcctcggaa actttattga
     2821 tccatgattt cgtgaatggt cggaacgtcg ggaatgcgtc cgtcgcgctt ttttggagtt
     2881 tgcatgatcg gccggacaaa t
//
LOCUS       DASQKS010000447         7902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1091935, whole genome shotgun sequence.
ACCESSION   DASQKS010000447 DASQKS010000000
VERSION     DASQKS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091935"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VE170_11535"
     CDS             complement(<1..661)
                     /locus_tag="VE170_11535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16113.1"
                     /translation="MKRQLPSIVLLTAMSFLLPPASYSAEAMQKIRVGLPSLALTYMP
                     FYVAQEKGLLKKLGLEAEYIQMNTGIQPQAIMGGNINFFPSLATGISAAVAGLPLVVV
                     LNFYNFTPWMLVTSKEINKPQDLIGKKVAISGIRTTGHYLMVAWFKKFEINEKDVGFV
                     MTGGTASSFASLASEQVAGAVLTPPFDDKAVSKGFKKYMLIGDLMDIPTSGLVASKAE
                     VS"
     gene            911..2035
                     /locus_tag="VE170_11540"
     CDS             911..2035
                     /locus_tag="VE170_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16114.1"
                     /translation="MAKTLLILLFILGAWANSFGADARPAWRIEWDKTVTAAEKEGAV
                     SIYIFEAGPLTEETVLAFERAYPKIKVNQLRGRGNDLGPRIVAERRAGKYLVDLFAGG
                     KGTALGTLYVGKLLDPIRPLLLLPDVLDETKWWRREHKYVDPENKYIFAYVGNAGGVE
                     INYNSNSVNPKEFTSYWDLTQPKWKGKIAATDPRSRGMDNPVLFFYYHARLGPEFIRK
                     LYGDMEVALSRDYRQPVDWLASGRYSLCIPCVSDEMDKAMDQGLPVGQVLRLKEGGTL
                     SSSGGTLTLLQNAPHPNAAKVFVNWILSREGQTVVQKGRKGRTRTASNSLRTDIAKND
                     LPEEVQRREGIDYFDADDDNFADRRPADKLFNEIFGKTAK"
     gene            2237..3244
                     /locus_tag="VE170_11545"
     CDS             2237..3244
                     /locus_tag="VE170_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16115.1"
                     /translation="MTKIVGRSAVLALVLSLLVFATHGAAQDKPLKKIYWGVSTLSPT
                     LWIPWIAKEAKIFEKNGLDVEAVLLNGSGRTSQALLSGSLFAASVALPQVMLADLTGA
                     DLANVAHGIGVQASRLMVRPEIRKVEDLKGKRIGISSLGSAGDLLFGYVLRKYGIDTT
                     REIIWLAVGNTAERLQALITGTVDAADMTYPADVEAERKGFRPLLDARKEIVYPTASV
                     VTRRKTIREERDTVMRFVRSFVEGIAYLKQNKEFSKKVLTKYMRTTDAEYLEGSYNMF
                     REDFISVPYPITKNLDAIYEIAAFRRPEIRTHKPEEFVDPSFVAELEKSGFIKKLYER
                     K"
     gene            complement(3383..4447)
                     /locus_tag="VE170_11550"
     CDS             complement(3383..4447)
                     /locus_tag="VE170_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16116.1"
                     /translation="MPILIFILSALTLSSMLFPGEVDAQPRKPQTASELVTYNGKDRE
                     AVLYAGAKSEGKATWYTSLAGDSYKAMIKSFETKYAGVKIEAYRANGGEITNRMFEEA
                     KARKSIVDTIETTESNLMFQREAFLLRPYHSPYFASYPEDAKVKGERGLYFWGTARES
                     YIGFGYNKNLLPKDAVPKNYQGLLHPALKGRMGLTLDDTSNKSIAGMLKTRGPEFLKK
                     LKGQDIPLHTIIPPALLDLIASGELIAGAAIFRNHVIVAQQKGAPVEWVAMDVVPTNV
                     GGVAIAAQPPHPHAALLLADYLLGPEGQSILEKYHYGNGAKNYGFKKWRPERDLPLDK
                     YEKELERWDKLLKETGRKGA"
     gene            complement(4515..5567)
                     /locus_tag="VE170_11555"
     CDS             complement(4515..5567)
                     /locus_tag="VE170_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16117.1"
                     /translation="MHFLIFALSVGSILFPAGLLAQLKPQTISELVTYNGKDREAMLY
                     AGAKSEGKVTWYTSLSGDSYKGMAKAFEAKYPGVKLDAYRVSGSDMTVRMMEESKAKR
                     YIVDTVETTEGNLMFMRDAFLLRPYHSPHFARYPEEAKEKGERGLYFWGIARESYIGF
                     AYNKKLLSKNAVPKNYEGLLHPELKGRMGVSVSDPAYKVIGAMLRTKGQEFVKKLKVQ
                     EIALHTIIPPALLELIASGEVLASPGIFRNHTLNAISKGAPVEWVPMDIVPTNVGGAA
                     IAVQPPHPHGALLLADYLLGGEGQAVLEKYEYGSAAKKLGFNKWRPDHGITTDKYEKE
                     LERWEKLLKEISRRGA"
     gene            complement(5590..6642)
                     /locus_tag="VE170_11560"
     CDS             complement(5590..6642)
                     /locus_tag="VE170_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16118.1"
                     /translation="MHSLDIIALLYVGSVLCSAHLHAQARKPTTIADLAAHSGKDREQ
                     LLYSGAKSEGKLTWYTSLAGDSYKAVARAFEVKYPAVRLEVYRAGGTDFIMRMIEEVK
                     ARRPACDTLETSEDSLLLARANNLLQPYFSPHLSKYPDDAMEKAGKGWSHWTTIRESY
                     LGFGYNKTQIPAHLVPKNFDGLLHPELKGKLSITLNESATRAIGAMIKVKDEAFVRKL
                     KGQEIKAYTVSSAAMADLIASGEIAGSFQIFRNHALVSAERGSALGWVPMELVPTNAG
                     SVALAAHAPHPHGAPLLIDFLLTDAPKILDKFNYGHPATDYGFKRWYQDQGRSLEDYE
                     KDSIKWGKLAREISQR"
     gene            complement(6665..7792)
                     /locus_tag="VE170_11565"
     CDS             complement(6665..7792)
                     /locus_tag="VE170_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16119.1"
                     /translation="MHHVIRAEEICVASRTGQQRHREIIVMLHALSGILILSFLCANQ
                     VFAQTRKPATVSEISTYMGADREQLLYAGAKSEGVVTWYTSLAGDSYKTLARAFETKY
                     PGVRVEAFRAGGSELVTRMSEETKARRPTMDALETTYDFTMVSKAYGLTRPYSSPLLA
                     SYPADAKEKADAGLTFWTIFRESYMGFGFSKDQVPADAVPKGFDGLLDPQLRGKMGIT
                     LNESSARMIGAMIKIKGEAFVKKLKAQQIRIYTVSSAALVDLMASGELGASFHIYRNH
                     AMVSAERGSPASWVPMELVPTNSGCVSLGAQPPHPHAATLFVDFLLGAESRKVLEKFQ
                     YGHPSNTLPFKRWHPGFGRSLEQFEKDSEKWEKLAKEITQR"
BASE COUNT         1603 a         2127 c         2344 g         1828 t
ORIGIN      
        1 tcgagacctc ggctttcgac gcaaccagac cggaagtcgg tatgtccatc aggtcgccga
       61 tcaacatata ctttttgaaa cctttggaaa ccgctttgtc gtcgaagggc ggggttagca
      121 cggcgccggc gacttgctcg ctcgccagcg acgcgaagct cgacgctgtc ccgccggtca
      181 taacaaaacc gacatccttc tcgttgattt caaatttttt gaaccaagcc accatcaagt
      241 agtggcccgt ggttcggata cccgagatcg caaccttttt gccgatcaga tcctgcggct
      301 tgtttatttc tttgctggtg accagcatcc acggcgtaaa attgtagaaa ttaagcacaa
      361 ccacgagcgg cagtccggcg actgccgcgg aaattccagt tgccagggag ggaaaaaaat
      421 tgatattgcc gcccataatc gcctggggct ggatgcccgt gttcatctga atgtactccg
      481 cttccaggcc gagtttcttg agcaagcctt tttcttgggc gacgtagaaa ggcatgtagg
      541 tgagggcgag ggacggcagg ccgacgcgga ttttttgcat cgcctcagcg ctgtagctcg
      601 cgggcggcag caaaaaactc attgccgtca ataggacgat cgatggcagt tgtcgtttca
      661 tgattgaatc cttcacacag tgctgtccga ttatgtttgg agagagtctc ctcagcctgc
      721 gctggcgatc ttaatcaagg gggagagcgg ccgtcaaact cgcgcttatg cgcgctaacc
      781 acatgggcga taagcgttca atgccttgtc ggtcgagcag ccgtgttttt gaatttggcg
      841 acaattgttg acagccagca acgccgctct tagagtgggc accggctcct tcgaggtgtg
      901 attattgaag atggcaaaga cactccttat ccttctattc atactcggtg catgggcaaa
      961 cagtttcggg gcagacgcgc ggcccgcttg gcgcatcgaa tgggacaaga ccgtgaccgc
     1021 ggctgagaag gagggtgcgg tttcgattta cattttcgaa gccgggccgc tcaccgaaga
     1081 aaccgtgctt gccttcgaac gcgcctatcc gaagattaag gttaaccaat tgcgcggccg
     1141 cggcaacgat ctcggtccgc gcatcgtcgc cgagcggcgt gccggcaaat atctagtcga
     1201 tctcttcgcc ggtggcaaag gcacggcgtt gggcacgctt tacgtcggca aacttttaga
     1261 tccaatccgg ccgcttttgt tgctgccgga tgtcttggat gaaaccaagt ggtggcgccg
     1321 cgaacataaa tacgtcgatc cggaaaataa atatattttc gcttatgtcg gcaacgccgg
     1381 cggtgtggag atcaactata attctaactc ggttaaccct aaggagttta cttcctattg
     1441 ggatctgacc cagcccaagt ggaaaggcaa aatcgcggct accgacccgc gttcccgcgg
     1501 catggacaat ccggtgctgt ttttctacta ccatgccaga cttgggcccg agttcattcg
     1561 caaactttac ggcgacatgg aggtcgcgct ctcgcgcgac tatcgccagc cggtggattg
     1621 gctggcatct ggacgttact ccctatgcat tccctgtgtt tcggatgaaa tggacaaggc
     1681 gatggatcaa ggtctgccgg tagggcaagt tctccggctc aaagaaggcg gcaccctgag
     1741 ttcatccggt gggacgctga cgttgctgca aaatgcgccc catcccaatg cggcgaaggt
     1801 atttgtcaac tggatattat ctcgggaagg acaaaccgtg gtgcaaaaag gccgcaaggg
     1861 tcgtactcgc actgcatcca attcactgcg aaccgatatt gccaagaacg atctgccgga
     1921 agaagttcaa cgccgagagg gcattgacta cttcgatgcg gacgatgata acttcgccga
     1981 ccggcgcccg gcggacaagt tgttcaatga gattttcggc aagacagcta aatagagatt
     2041 ggccgattag acacgaatgt ccagcgcggt taagccgcac ccgaaaataa gaatcgccgc
     2101 aaagaacgca aaggacgcaa aatagaattg ggagttgtcg ggacggggtt tgaaagccct
     2161 cttaagtttg ctattggcgg atcatttcgc gcggtgagta gttcagcgaa cagttagatt
     2221 gcgccaggga gatgccatga ccaagatcgt tgggcgaagt gccgtgctgg ctttggttct
     2281 gtctctgctt gttttcgcga cccatggtgc ggctcaagac aaaccgctca agaagattta
     2341 ttggggcgtg tcgacgttgt cgccgacctt gtggatccct tggatcgcca aggaggcgaa
     2401 gatttttgag aagaacggcc tcgatgtcga agcagttttg ctcaatggct caggacgaac
     2461 ttcacaggcg ctcttgtccg gcagtttgtt tgccgcgtcg gtggccttgc cgcaagtcat
     2521 gctggcggat ttgaccggcg cggacttggc caatgtcgcc catggtatcg gtgtacaggc
     2581 gagccgtctc atggtgcggc cggagattcg caaggtcgaa gatctcaagg ggaaacggat
     2641 cggcatatcg agcttgggtt ccgccggcga tctgttgttc ggctacgtgc tgcgcaagta
     2701 cggcatagac acgacccgcg agatcatctg gctagcggtc ggcaataccg ccgagcggct
     2761 gcaagcactc ataaccggga cggtcgatgc ggcggacatg acctatcccg ccgacgttga
     2821 ggcggaacgc aaaggctttc gccccttgct cgatgcgcgc aaagaaattg tctatccgac
     2881 ggcgtcggtg gtgacgcggc gcaagacgat acgcgaagag cgcgataccg tgatgcgctt
     2941 cgtgcgttcg tttgtcgaag gcatcgccta tctcaagcaa aataaagaat tcagcaaaaa
     3001 agtgctgacc aaatacatgc gcacgaccga tgcggaatat cttgaggggt cctacaacat
     3061 gtttcgggaa gatttcattt ccgtgcccta tccgatcacc aaaaatctcg atgcgatcta
     3121 cgagatcgcg gcgtttcgcc gcccggagat ccgcacgcac aaacccgagg agttcgtcga
     3181 cccgagcttt gtcgccgaac tcgagaagag cggcttcatc aaaaagcttt atgagaggaa
     3241 gtaggaggga atggccgcaa agaacgcaaa ggacgcaaaa agaattggga cagccccgac
     3301 aaaatccgaa gccctttttg cgaactatgc gttctttgcg gctaaatctt ttgtctcatt
     3361 tggttaccag ttacggcacc attcacgcgc ctttgcggcc ggtttccttc agtagcttgt
     3421 cccaccgttc gagttctttt tcgtatttat ccagcggcag atcgcgttcg ggacgccatt
     3481 ttttaaagcc gtaatttttt gcgccgttgc cgtagtggta tttctccagg atgctttggc
     3541 cttccggccc gagcaggtag tcggcgagca gcaacgcggc gtgcgggtgg ggtggttgcg
     3601 cggcgatggc gacgccgccg acgttggtcg gcaccacatc catggcgacc cattccaccg
     3661 gcgcgccttt ttgttgggcg acgatcacat ggttacgaaa gatcgccgcg ccggcgatca
     3721 attctcccga ggcgatgaga tcgagcaacg ctggcgggat gatcgtgtgc agagggatat
     3781 cctggccttt gagttttttc agaaattcag gcccgcgggt tttcagcatg ccggcgatgg
     3841 acttgttact ggtgtcgtcg agggtgaggc ccattctgcc cttgagggcg ggatgcagca
     3901 gcccttggta atttttcggc acggcgtctt tgggcagcag gttcttgtta tagccgaagc
     3961 cgatgtagga ctcccgcgcc gtgccccaga agtagaggcc gcgttcgcct ttgaccttgg
     4021 cgtcctcggg ataggaggca aaatagggag agtgataagg ccgtagcaaa aacgcctcgc
     4081 gctggaacat taagtttgat tcggtggttt cgatggtgtc gacgatggac tttctcgcct
     4141 tggcttcctc gaacatacgg ttggtgatct cgccgccgtt ggcgcggtag gcttcgattt
     4201 tgacgccggc atattttgtc tcgaaggact tgatcatggc cttgtaggaa tcgccggcga
     4261 gggacgtata ccaggttgct ttcccttcgc tcttcgcgcc ggcgtagagc accgcttcgc
     4321 ggtctttgcc gttgtaggtg accagctcgg aagcggtctg cggtttgcgc ggctgagcgt
     4381 cgacttcgcc ggggaatagc atggaactga gtgttagggc tgacaggatg aatattaata
     4441 tgggcatgat tgtatggctc tctcctgcaa aactcttatc gtgcgccggc tccgctgaca
     4501 agccgggctc actcttaagc gccgcggcgg ctgatttctt ttaaaagctt ttcccagcgc
     4561 tccagctcct tttcgtattt gtcggtagtg atgccatggt cggggcgcca tttgttgaaa
     4621 ccgagtttct tcgctgcgct gccgtattca tatttctcca agaccgcttg gccttcaccg
     4681 ccgaggagat agtcggctaa cagcaaagcg ccgtgcgggt gcggcggttg cacggcaatg
     4741 gcggcgccgc cgacattggt cggcacgatg tccatgggca cccactccac cggcgcgcct
     4801 ttgctgatcg cgttcaacgt gtggttgcga aagatgccgg gcgacgccag cacttcaccg
     4861 gacgcgatca attccaacag cgccggcgga atgatcgtgt gcagggcgat ttcctgcacc
     4921 tttagttttt tgacaaactc ttggcccttg gtgcgcagca tggcgccgat gactttgtag
     4981 gcgggatcgc tgaccgacac gcccatgcgg cctttgagct ccggatggag cagcccttca
     5041 tagtttttcg gcacggcgtt tttcgacagg agcttcttgt tgtaggcaaa accgatatag
     5101 gactcccgcg cgatgcccca gaaatataat ccgcgctcgc ctttttcctt ggcctcctct
     5161 ggatagcggg cgaagtgggg tgaatgatag ggacgcaaga gaaaggcatc gcgcatgaac
     5221 atcaagttgc cttcggtggt ttcgacggta tcgacgatgt aacgcttggc cttcgactcc
     5281 tccatcatgc gcacggtcat gtcggagccc gagacgcggt aggcatcgag cttgacgccg
     5341 ggatacttgg cttcgaaggc ttttgccatg cccttatacg agtcgcccga cagcgacgtg
     5401 taccaggtga ctttgccttc gctcttggcg ccggcgtaga gcatcgcctc gcggtctttg
     5461 ccgttgtagg tgacgagctc ggagatggtc tggggcttga gttgggcgag caggccggcg
     5521 gggaacagga tcgatccgac cgatagggcg aatattaaaa aatgcatcgc gataggctcc
     5581 tttggtgcgt tagcgttggc tgatttctct ggcgagtttg ccccacttta tactgtcctt
     5641 ctcataatct tccagggatc gaccctggtc ttggtaccat cttttaaaac cataatccgt
     5701 tgccggatgg ccgtagttga atttatcgag gatcttcggc gcgtcggtga gcagaaagtc
     5761 gatcagcaac ggtgcgccat ggggatgagg cgcgtgggcc gccagcgcca ccgagccggc
     5821 gttggtcggg accagttcca tcggcaccca gccaagcgct gaaccgcgtt cggctgagac
     5881 cagagcgtga ttgcgaaaga tttgaaacga tccagcgatt tcgccagagg cgatcaagtc
     5941 ggccatggcg gcgctgctga cggtataggc tttgatctcc tggcccttga gcttcctgac
     6001 gaacgcttcg tctttcactt tgatcatggc gccgatggcg cgcgtcgccg actcgttcag
     6061 ggtgatgctc aatttgccct tgagctcggg atgtaacaag ccgtcgaaat ttttcggcac
     6121 caggtgggcc ggaatctgcg tcttattgta gccgaagcct aaataagact ctcggatggt
     6181 ggtccagtga ctccacccct tgcccgcttt ttccatggcg tcgtcgggat acttgctcaa
     6241 atgcggcgag aagtaaggct ggagtaaatt attcgcgcgc gccaacagta gcgaatcctc
     6301 agaggtctcc aatgtgtcgc aagccggccg gcgcgctttg acttcttcga tcatgcgcat
     6361 gatgaaatcg gtgccgccgg cgcgatagac ctccagccgc acggcgggat atttgacctc
     6421 gaaggcgcgc gcgacggctt tataggagtc gccggcaagt gacgtgtacc aggtaagttt
     6481 gccttcgctc ttggcaccgc tgtaaagcag ctgctcgcgg tctttgccac tatgggcggc
     6541 cagatcggcg atcgtcgtcg gcttgcgcgc ctgggcgtgg agatgcgccg agcataggac
     6601 cgatccgaca tataggaggg ctatgatgtc tagagagtgc atcgcggaag tgacctgtgc
     6661 ggcgtcaccg ttgggtgatt tccttggcga gcttttccca cttctcgctg tctttctcga
     6721 attgttcgag ggagcggccg aagccgggat gccaacgttt gaacggcagg gtgttggacg
     6781 ggtgaccgta ttgaaacttc tccaagacct tgcgcgattc ggcgccgagc agaaagtcga
     6841 caaacagcgt cgctgcgtgg gggtgcggcg gctgggcgcc gaggctgacg cagccagagt
     6901 tggtcggcac cagctccatc ggcacccagc tcgccggcga tccgcgctcg gccgagacca
     6961 tggcgtgatt tctgtaaata tgaaacgatg cgccgagctc gcctgacgcc attagatcca
     7021 ccagcgcggc gctgctgacg gtataaatgc ggatctgctg cgccttgagt tttttgacaa
     7081 aggcttcgcc cttaatttta atcatggcgc cgatcatgcg cgccgaactt tcattgagcg
     7141 tgatgcccat tttccctctg agctgcggat cgagcaggcc atcgaaccct tttggcacgg
     7201 catcggccgg cacctgatcc ttgctgaaac cgaagcccat gtatgactcg cgaaagatcg
     7261 tccagaaagt taggccggca tctgccttct ctttcgcatc cgctgggtaa ctcgcaagca
     7321 gcggcgaact gtacggccgg gtcagaccgt aggccttcga caccatggtg aaatcgtaag
     7381 tggtttcgag cgcatccatg gtcggccggc gcgccttggt ttcctccgac atgcgcgtga
     7441 ccagttcgct gccgccggcg cgaaacgctt cgacccgcac ccccggatat ttagtttcga
     7501 aggcccgtgc cagcgtcttg taggagtccc ccgccagcga ggtgtaccac gtgactacgc
     7561 cctcgctctt ggcgccggca tagagtagct gctcgcggtc cgcgcccatg taggtggaga
     7621 tttccgatac ggtggcgggt ttgcgcgtct gggcgaagac ttgattggcg caaagaaacg
     7681 ataaaattaa tatgccggat agcgcatgca acatgacaat gatctccctg tgcctttgct
     7741 ggccggtgcg gctggcgacg catatctctt ccgcccggat aacatggtgc atcgaagcga
     7801 gtcaatgcgc aggcgatcct tcttcccaaa agggggcgat cgtcaactta cccgaatggg
     7861 gcttgtggga agttttcgta cttgttatcg ctctgaccgg ct
//
LOCUS       DASQKS010000448         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3617383, whole genome shotgun sequence.
ACCESSION   DASQKS010000448 DASQKS010000000
VERSION     DASQKS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617383"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VE170_11570"
     CDS             <1..389
                     /locus_tag="VE170_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HYJ16120.1"
                     /translation="TFIGPWTKSGDKYSESDDGYFTYGGRADDMLKVSGIWVSPFEVE
                     SALASHTAVLEAAVVGQADDNGLIKPKAFVVLRSDTPQSTELLSALQQHVKGQLAPYK
                     YPRWIEFVAELPKTATGKIQRFKLRA"
     gene            491..1615
                     /locus_tag="VE170_11575"
     CDS             491..1615
                     /locus_tag="VE170_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16121.1"
                     /translation="MLLPQSFAIAQAQRIRVGLMLPYSGTYAQLGTAITNGFKLAVNE
                     RGGKLGGRELEFFTVDDEAEPSKAPENTNKLVQRDKVDVLVGTVHSGVQMGMVKIAKE
                     TNTLMIIPNAGLDAATGALCAANIFRTSFSNWQPSYPMGKVLADRGIRNVVSLAWKYG
                     AGEEAVAGFTEGFIKAGGKSVRGLWIPFPNAEFQALLTEIASLKPDAVFVFFAGAGAA
                     KFVKDYAAAGLRKTIPLYGSGFLTDGILDAIQDSGQGLETTLHYADGLNSKRDKEFRA
                     AYTKAFTSEPDVYAVQGYDAALLLTTGLDAVKGDISKRKEMIAAMEKAKIDSPRGPWT
                     MSKAHNPIQDIYLRKVVGKENRLVSIAHKALADPARGCKL"
     gene            1722..2594
                     /locus_tag="VE170_11580"
     CDS             1722..2594
                     /locus_tag="VE170_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYJ16122.1"
                     /translation="MNVLNMFYLIQLLNAVQYGLLLFLLSSGLTLIFGIMGVINLAHG
                     SFYMVGAYLTYWLAKTTGSLWFAIPIGIAIAFAIGLLLETTIIRRLYKRDHLYQVLLT
                     FGLILIFEEVRSILFGDEVHGVAVPELLNYSIPLTANLAYPVYRLFVTAICLIVAIVM
                     YLVIHKTRAGMMIRAGNSNRDMAATLGVNIPLLFALVFACGMALAAFAGAIAAPITSV
                     APGMGNQVLIICFVVVVIGGIGSINGALIASLFIGLADTLGKVLAPEYSGVAVYLLMA
                     AILLWRPQGLANRS"
     gene            2591..3601
                     /locus_tag="VE170_11585"
     CDS             2591..3601
                     /locus_tag="VE170_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYJ16123.1"
                     /translation="MTRITLPRWWLLGIVLLLAIVPLGATTFYLQLGAKIMIMAIFAL
                     SLDLLIGHTGLVSFGHSAYFGVAAYALALITPKYEAASFWLTLPAAMTAAALAALVIG
                     VLVVRTAGIYFIMVTLAFAQMLYAIFHDTNLGGGSDGIYINVRPELNVAGFKPLDLES
                     PAQFFYLALIALIAVYWSLNKILHAPFGRALAGIRVNEQRMRAIGFPVFFYKLGAFTL
                     AGALAGLAGYLAACQFGFVNPEILAWHHSGTVLMMVILGGMGRLHGAVIGAAAYVLLQ
                     ELFSSSALFGAYAKHWQLFMGGLIVFIVLALPHGIGGLIDNAWVAWRRRNGGLNPEST
                     DG"
     gene            3594..>3988
                     /locus_tag="VE170_11590"
     CDS             3594..>3988
                     /locus_tag="VE170_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ16124.1"
                     /translation="MAEAILHVRNLTKHFDGLAAVENISLEVHRREIHAVIGPNGAGK
                     STLINLLAGELRASAGEINFQGRDISSLASEKRSRIGIGRSFQKTNIFPSFTALENCR
                     LAAQSREPRPWRWLRDAQSYQSIMSKAHQA"
BASE COUNT          792 a         1202 c         1083 g          911 t
ORIGIN      
        1 atactttcat cgggccctgg accaagagcg gcgacaagta cagcgagagc gatgacggct
       61 attttaccta cggtgggcgc gccgacgaca tgcttaaagt cagcggtatc tgggtgtcgc
      121 cgttcgaagt ggaatccgcc ctcgcgtccc acaccgccgt gctcgaagcc gccgtggtcg
      181 gccaggccga tgacaacggc ctgatcaaac cgaaggcttt cgtggtgctt cgctcggaca
      241 ccccgcaaag caccgagctc cttagcgcgc tccagcaaca tgtcaaaggc caactggccc
      301 cctacaaata tccgcgctgg atcgaattcg tcgccgaatt gcccaagacc gctacgggta
      361 aaattcaacg cttcaagctg cgcgcttgaa atccatctcg tttctggcac aaaagagcag
      421 ctattcggag gagacaatga aacttacgcg tcgtacattg ctgattcaga ccggcctgct
      481 catctggacc atgttgttgc cgcagtcctt cgccatcgcc caggcgcaac ggatccgcgt
      541 cggtctcatg ctgccgtact caggaaccta tgcgcagctc ggtaccgcaa ttaccaacgg
      601 cttcaaactg gcggtgaatg agcgtggcgg caaactcggc ggacgggaat tagaattctt
      661 caccgtcgac gacgaagccg aaccatccaa ggcgccggag aacaccaata agctagtcca
      721 gcgcgacaaa gtcgacgtgc tggtcggcac cgtgcacagc ggcgtgcaga tgggaatggt
      781 taagatagcc aaagaaacca acacgctgat gatcatccca aatgccggcc tcgacgccgc
      841 caccggagcg ctgtgcgcgg ctaatatttt tcgcacttcg ttttccaact ggcagccgtc
      901 ctatccgatg ggcaaggttc tcgccgaccg cggtatccgc aatgtcgttt cgctggcatg
      961 gaaatatggc gccggcgaag aagcggttgc cggcttcacc gaaggcttca tcaaagccgg
     1021 cggcaagtcc gtgcgtgggc tatggatccc attccccaat gctgaatttc aagcgttgct
     1081 cacggaaatc gcctcgctca aacccgatgc cgtgttcgta ttctttgccg gagccggtgc
     1141 tgccaagttc gtcaaggatt atgccgctgc aggtttacgg aaaactatac cgctctatgg
     1201 ctcgggattt ttgaccgacg gcatcttgga cgcgatccaa gactccgggc agggcctgga
     1261 aacgactttg cattacgcgg acggcttgaa cagcaaacgt gataaagagt tccgggccgc
     1321 gtacacgaaa gcattcacca gtgaacccga tgtctacgcg gtccaaggtt atgacgcggc
     1381 actgctccta accaccggcc tcgacgctgt gaaaggcgat attagcaagc gcaaggaaat
     1441 gatcgcggca atggaaaaag ccaagatcga cagcccacgc ggtccttgga ccatgtcgaa
     1501 ggcgcacaac ccgatccagg acatttactt gcgcaaagtc gtcggcaaag aaaatcgcct
     1561 cgtcagcatc gcgcacaaag cgctggccga cccggcccgg ggatgtaagt tatagcggcg
     1621 gtgattccgc cgctgcgttc aaaccgctca agtcgttcaa gccgttcaaa tcgttcgtag
     1681 aaatcggccc gaattctgac ggcttgaaca ttttaaacgg cttgaacgta ttgaacatgt
     1741 tctatttgat tcagttactg aacgcagtcc aatacggtct cctgctcttc cttctgtcca
     1801 gcggcctgac cctcattttc ggcatcatgg gcgtgatcaa tcttgcccat ggcagtttct
     1861 atatggttgg cgcctacctg acctattggc tggcgaaaac gaccggcagt ctatggttcg
     1921 ccatacccat cggcatcgcc atcgcttttg ccatcggtct attgcttgaa acaaccatca
     1981 tccgccggtt gtataagcgc gatcatctct atcaagtgtt gctcaccttc ggtctgatct
     2041 tgatcttcga agaggtgcgc agcattttgt tcggcgatga agttcatggc gttgccgtgc
     2101 cggagctctt gaattattcg atcccgttga ccgcgaactt ggcttatccc gtctaccggc
     2161 tcttcgtcac ggcgatctgc ttgatcgttg ccatcgtcat gtacttggtc attcacaaaa
     2221 cccgcgccgg catgatgatc cgcgctggca acagcaatcg tgacatggcc gcgacgctgg
     2281 gcgtcaacat tccgctgctg tttgctctgg ttttcgcctg cggcatggcg ctggcggcct
     2341 ttgccggtgc catcgctgcc ccgatcacgt cggttgcgcc gggcatgggc aatcaggttc
     2401 tgattatctg cttcgtggtc gtggtcatcg gcggcatcgg ctcgatcaat ggcgctctca
     2461 tcgcctcgct gtttatcggc ctcgccgaca ccctgggcaa agtgctggcg ccggaatatt
     2521 ccggcgtcgc cgtttatctt ttaatggcgg caattctgct gtggcggccg caggggctgg
     2581 ccaacagatc atgacccgga tcacgctccc gcgctggtgg ttgcttggca tcgtgctgct
     2641 actcgccatc gtgcccctgg gcgcgaccac cttttatctc caacttggcg ccaagatcat
     2701 gatcatggcg atcttcgcct tgagtctcga tctgctcatc ggccatacgg gattggttag
     2761 cttcggccac tccgcctact tcggcgtggc ggcctatgcg cttgccttga tcacacccaa
     2821 atatgaagcg gccagtttct ggctgacact tcccgcggcc atgaccgccg ccgcgcttgc
     2881 cgcgctcgtc attggggttt tggtcgtgcg taccgcgggc atctatttca tcatggttac
     2941 cctcgccttc gcacaaatgc tctatgcgat ctttcacgac accaatctgg gcggcggctc
     3001 cgatggcatt tacatcaacg tccggccgga attgaacgtt gccgggttca agcccctcga
     3061 tctcgaaagt ccggcgcagt ttttttactt ggcgctgatc gcattaatcg ccgtctattg
     3121 gagtttgaat aagattttgc acgcgccctt cggccgtgct ctcgccggta tccgtgtcaa
     3181 cgagcagcgc atgcgcgcca tcggttttcc ggtgttcttt tacaagctcg gcgccttcac
     3241 cctcgccggc gcccttgccg gtctcgccgg ctatctcgcc gcctgccagt tcggcttcgt
     3301 caatccggaa atcttggcat ggcaccactc cggcactgtg ctcatgatgg tgatcctggg
     3361 cggcatgggc cggctccacg gcgccgtcat cggcgcggcg gcgtatgtct tattgcagga
     3421 attattttcc tcgtcggctc tgttcggcgc ctatgccaag cactggcaac tgttcatggg
     3481 cggtttgatc gtcttcatcg tgctcgcgct accgcacggc atcggtggac tcatcgataa
     3541 tgcctgggtg gcctggcgcc gccgcaacgg cggtttgaac ccggagagca ccgatggctg
     3601 aggcgatttt acatgtgcgc aacctcacca aacacttcga cggcctggcc gccgtcgaaa
     3661 atatttctct ggaagttcat cgacgtgaga tccacgcagt catcggccct aatggcgccg
     3721 gcaaatcgac gttgatcaac ctgcttgcgg gagaactgcg cgcgtcagcg ggtgaaatca
     3781 atttccaagg acgcgatatc agctcgcttg cgtcggaaaa acgctcgcgt attggcatcg
     3841 gccgcagctt tcaaaagacc aacatattcc cgagtttcac agcactggaa aattgccgct
     3901 tggcagcgca atctcgcgaa ccgcgcccat ggcgttggtt acgcgatgct cagagctacc
     3961 aatcgatcat gtcgaaggca caccaagc
//
LOCUS       DASQKS010000449         5615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5699069, whole genome shotgun sequence.
ACCESSION   DASQKS010000449 DASQKS010000000
VERSION     DASQKS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5699069"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1359)
                     /locus_tag="VE170_11595"
     CDS             complement(166..1359)
                     /locus_tag="VE170_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYJ16125.1"
                     /translation="MSITNKAAIVGIGDTPTDRLGSKPGEPRKSSAEYLAWAMQLALA
                     DAGLSLKDFQGQGLGVTIPTAYPQPFWPEEVSEILGITPGFLLGGATGGAGAVSLIGG
                     MSAAINSGLIDLALVVGAAAPFSEHWGGGIQPGDMRDWEIPYGTMGPNSKIAMVMRRH
                     MHEYGTTLDHLGKIAVTARYHSSLNPLGYLRSPMTIEDYKNSRLVADPVRLFDCVMPA
                     NGGKAYILASPERAKSLRKKPVWVKGFGERSNPSYGPRAGSDALVMGVKDAGRVAMEM
                     AGVKHKDINFLQLYDDYVIVVYLQIEDLGFCQKGDLKFFENTDFTFKGQLPIQTGGGI
                     INCGQPSTAGGVHHILEGVRQLRGEGGDRQVPNAKIGLISGLGVLPYGKNLGCCAVAI
                     LGNEL"
     gene            1502..2491
                     /locus_tag="VE170_11600"
     CDS             1502..2491
                     /locus_tag="VE170_11600"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16126.1"
                     /translation="MINQLAKFLFALSLLLLPGAAQAQKFVQAYSSISTLNATFWIIQ
                     DAGFAKQEGLDTELVYIPSSSTVAQATLAGEVIISPANGQVIADVGLQGGDLVAMGGI
                     TNVVAFYIMATPEIKTIADLRGKTVGVTRFGSSGDVGMRMFLTKYGLEPVKDVPMIQL
                     GGLPEIAAAMGKRTVHAAAFSQPMAYVAQQGGAHLLANLAKENIPFLHVGVTTTRKFM
                     RERRAQAKAYLRAYGRAVHFMHTKKEDSKAIISKYTKIKDQGMLDGSLTYGYDFIEKV
                     PLVKAAAFQVTLDDIVKKNPKAKSAKPEQFYDNSLVQELIDEGFFVKLWGRVP"
     gene            2488..3486
                     /locus_tag="VE170_11605"
     CDS             2488..3486
                     /locus_tag="VE170_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16127.1"
                     /translation="MMENHASARGAIIFILLALWISPAQAQKLVIAWTAVSAFNAPFW
                     IMNDAGFYKEEGLDVDTMYIASSPVAAKATLAGDVVISAQNSQVIADSGLQGGDLVAI
                     GATVNIVPFYVMAAPEIKTVADLKGKTIGVSRFGAATDFGMRMFLAKHGLEAHKDVAF
                     IQIGGMPEIAAALSKKIIAAAPMSYPMVYVAEQGGAKVLANLAKDEIPFVHLTLTTTK
                     RFVKERRPAAKAFLRAYGKAMHFIYNRPVETKAIFAKYTKVTDPRILDGSLKYGYEFM
                     EKVPLVKAAGFQVTLDEIGRTNAKAKGAKPGQFFDNSLVQELVDEGFFVKLWGRNP"
     gene            3711..4802
                     /locus_tag="VE170_11610"
     CDS             3711..4802
                     /locus_tag="VE170_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ16128.1"
                     /translation="MPLFLNNDEVGQLITMKDTMEALEILYGEMGQGAAVTAPRSDVH
                     SPTAAAATAEGPMAHYLKSMSGASPHFGTAALRFSSDIVAWRDSAGGMRREKLPLLPG
                     QRWMGVILLFSSANGELLAVMNDGVLQRFRVGGANGVSTKYLARANAETVGLIGSGWQ
                     AGTQVLAVCEARKIKRINVYSPTKANRENFAKETSAQVGVEIVPVDSYEAAVKDVDIL
                     ITSTNARRPFLGKWVLREGLHISAMQRDEFDDEALMACQPLVLHTHSTENNVTSSALA
                     HFERDDFTLRDHPTTRSIDWQSLPTLPDLICRRIKGRESDRQITGFVNNIGMGAQFAA
                     VGKKVYDAAQAKGLGREVPGEWFTQDVHP"
BASE COUNT         1252 a         1514 c         1655 g         1194 t
ORIGIN      
        1 ttggcagcgc tggatggata gttgttgttg cgccgcggca ttccaaaagg ctcgcgcttc
       61 gttggtgatc gccggcaggg gttttcttac gtctagttca gccatgattt tattcttttc
      121 gccgaggtcg cgtttcaatg agcgcaacgc gaacggtttt tgtgtctaca gttcgttgcc
      181 caagatcgcg acggcacagc agccaagatt tttgccgtag ggcaggacac ccaggcctga
      241 gatcaagccg atcttggcgt tgggcacctg acggtcgccg ccttcgccgc gcaactggcg
      301 cacgccttct aagatatgat gcacgccgcc ggcggtggaa ggctggccgc aattgataat
      361 cccgccgccg gtttggatcg gcagttggcc tttgaaagta aagtcggtgt tctcgaagaa
      421 tttaagatcg cccttttggc aaaagcccaa gtcttcgatc tgcaagtaga ccacgatcac
      481 gtagtcgtca tagagctgca aaaaattaat gtccttgtgt tttaccccgg ccatctccat
      541 ggcgacgcgg ccggcgtctt tgacgcccat aacaagagca tcggacccgg cgcgcggccc
      601 gtaggagggg ttgctgcgtt cgccaaagcc tttgacccac accggctttt tgcgcaatga
      661 tttggcgcgc tcaggcgagg ccaagatata ggccttgccg ccgttagcag gcatcacgca
      721 atcgaatagg cgcaccggat cggcgaccaa gcgggaattc ttataatcct caatcgtcat
      781 cggtgaacgt aggtagccga gcggattcaa gctcgagtga tagcgcgccg tgacggcgat
      841 tttgccgaga tggtcgaggg tggtgccgta ttcatgcata tgccggcgca tgaccatggc
      901 gattttgcta ttgggcccca tggtgccgta gggaatttcc cagtcgcgca tgtcgccagg
      961 ctgaatgccg ccgccccaat gttcggagaa cggcgcggct gcgccaacca cgagcgcgag
     1021 atcgatcagt cccgagttga tcgccgcgct catgccgccg atgagcgaca ccgcaccggc
     1081 gccgccggtg gcgccgccga gcaggaagcc cggcgtgata ccgaggattt cgctgacttc
     1141 ttcgggccag aacggctgcg gataggccgt gggaatcgtc acgccgagcc cttgtccttg
     1201 aaaatccttg aggctcaggc cggcgtccgc cagcgcgagc tgcatggccc aggctaaata
     1261 ttcggcgctc gatttgcgcg gctcgccggg cttcgagccc aagcgatccg tcggggtgtc
     1321 gccgatgcca acgattgctg ctttgtttgt tatgctcata gtccgtccaa tttggtttca
     1381 gccgggacct taggatagct gtcgaatcat ttcaagggaa atccagtttg gcttgacagc
     1441 gttgtgaggt gccgatacca ctgctggaaa agtattggac gaatcaacgg ataggacgga
     1501 tatgatcaat caattggcaa aattcttatt cgcgctttcc ttgctgctat tgcccggcgc
     1561 ggcgcaggcg caaaagttcg tgcaggcgta ctcgtcgatt agcacactca acgcgacctt
     1621 ttggatcatc caagacgccg ggttcgccaa gcaggaaggg ctcgacaccg aattggttta
     1681 catacccagt tcgtcgacgg tggcgcaggc gacgcttgcc ggtgaagtga tcatatcacc
     1741 ggccaacggc caagtaatcg ccgatgtcgg cctgcaaggc ggcgatttgg tcgccatggg
     1801 cggcatcacc aatgtcgtgg cgttctacat catggcgacg ccggaaatca aaaccatcgc
     1861 cgatctcaga ggaaaaaccg tcggtgtgac acgcttcggc tcttccggcg atgtcggcat
     1921 gcgcatgttc ctgaccaagt acggcctgga accggtgaaa gacgtgccga tgattcaact
     1981 cggcgggctg cccgagatcg ccgcagcgat gggcaagcgc acggtgcatg ccgcggcgtt
     2041 ttcccaaccc atggcttacg tcgcgcaaca gggcggcgcg catttattgg cgaatctggc
     2101 gaaggaaaac attccctttc tccatgtcgg cgtgaccacg acgcgcaaat ttatgcgcga
     2161 acggcgcgcc caggccaaag cttatctgcg cgcctacggc cgcgccgtcc atttcatgca
     2221 tacgaaaaaa gaagacagca aggcgattat cagcaaatac accaagatca aagatcaagg
     2281 catgctggat ggcagtctga cctatggtta cgacttcatc gaaaaagtgc cgctggtcaa
     2341 agccgctgct ttccaggtca cgctcgacga catcgtcaaa aagaatccta aagcgaagag
     2401 tgccaagccg gaacagttct acgacaacag cctggtgcag gaactgatcg atgaaggttt
     2461 cttcgttaag ctgtggggga gagtgccatg atggagaatc atgccagtgc tcgtggcgcg
     2521 ataatattca ttctgcttgc gctgtggatt tcgccggccc aagcccaaaa gctggtcatt
     2581 gcctggacgg cggtgagcgc gttcaatgcg ccgttttgga tcatgaacga cgcgggattc
     2641 tacaaagaag aaggtttgga cgtcgatacc atgtatatcg ccagctcgcc ggtggcggcg
     2701 aaagcgacgc tggccggtga tgtggttatc agcgcgcaaa atagccaggt aattgcggac
     2761 tcggggctcc aaggcggcga cttggtggcg atcggggcga cggtgaatat cgtgcctttc
     2821 tatgttatgg ccgcgcctga gataaaaacc gtcgctgacc tcaaaggcaa aactatcggc
     2881 gtctcgcgtt ttggcgcagc cactgacttc ggtatgcgca tgtttctggc caagcatggg
     2941 ctcgaagctc ataaggacgt agcgtttatt caaatcggcg gcatgcccga gatcgccgcg
     3001 gcgctgtcca agaagattat cgcggcggcg ccgatgtctt atccgatggt ctatgtcgct
     3061 gaacagggag gggcaaaggt gctggccaat ctcgccaagg atgagattcc cttcgttcac
     3121 ttgactctga cgaccactaa acgcttcgtc aaagaaaggc ggccggcggc gaaagctttt
     3181 ttgcgcgcct acggtaaggc gatgcatttc atttataacc gaccggtcga gacgaaggcg
     3241 atattcgcta aatacaccaa ggtcactgat ccaagaatcc tcgacggcag cttgaagtac
     3301 ggctacgagt tcatggagaa agtgccgttg gtcaaagcgg cgggtttcca agtgacactc
     3361 gatgaaattg gccgcaccaa tgccaaggca aagggtgcca agccggggca attcttcgac
     3421 aacagtctgg tgcaagaatt ggtcgacgaa ggattcttcg tgaaattgtg gggccgcaat
     3481 ccgtaaaccg taatactcac cacgaagtgt ttcatgatct tacgctcgtc tgcagaatat
     3541 gaagatggct gggattccct gcatcggcgg gctgcttgac gcgtcgacgg agtggttctt
     3601 gcgggatata cgggattagt gcaccgacca tcggggatgt ttgaatctaa acattcctcg
     3661 gagcttgccg cggggcaccg cagggggcga gggaactttt cgaggtatac atgccactat
     3721 ttctcaacaa cgacgaagtc ggtcaattga tcaccatgaa ggacaccatg gaggcattgg
     3781 agattctcta tggtgaaatg gggcagggcg cggctgtcac cgcgccgcgc tccgatgtcc
     3841 atagtccgac tgccgcggcg gcgaccgctg aggggccgat ggcgcactat ttaaagagta
     3901 tgagcggcgc cagtccgcac tttggcaccg cggcgctgcg cttttcctcc gacatcgtcg
     3961 cctggcgcga cagcgccggt ggcatgcgcc gggaaaagct accgctgttg ccggggcagc
     4021 gttggatggg agtgatttta cttttcagtt cggccaacgg tgaattgctg gcggtgatga
     4081 atgacggcgt cttgcaacgc ttccgcgtcg gcggagccaa cggcgtgtcg accaaatacc
     4141 tggcgcgggc caatgccgaa accgtcggcc tgatcggttc cggctggcag gccggcaccc
     4201 aggtgttagc cgtatgcgag gcgcgcaaga tcaagcgcat caacgtctac agtccgacca
     4261 aagccaaccg cgaaaatttc gccaaggaaa ccagcgccca agtcggcgtt gagatcgtcc
     4321 ccgtggactc ctacgaggcg gcggtcaagg atgtcgacat cctgatcaca tcgaccaatg
     4381 cgcgcaggcc gtttctcggc aagtgggtgt tgcgcgaagg cttgcacatc agcgccatgc
     4441 agcgcgatga attcgacgac gaagccttaa tggcctgcca accattagtg ctccacactc
     4501 actcgactga gaacaatgtg acttcttcgg cgctggccca tttcgagcgc gacgatttca
     4561 ccttgcgcga ccatccaacg acgcgctcca tcgattggca gtctctgccg accttgccgg
     4621 atctcatttg cagacggatc aagggacgcg aaagcgaccg gcaaatcacc ggcttcgtaa
     4681 ataacatcgg catgggtgcg caattcgccg cggtcggtaa gaaggtttac gatgccgcgc
     4741 aggccaaggg actgggacgg gaagtgccgg gggagtggtt tacccaggat gtccatccgt
     4801 agcggtgtcc ggccgaaagt tgcctgttta aaatttctag tttcagattt gatactccaa
     4861 gccaaacagc ttgaaataga atgaattttg tcttagcctg ggtgcctacc tacttcttat
     4921 catgcgcagg ttgggcgcag aataagttcc ccctcagtta ttcatattca gtttccaatc
     4981 aaacgctgct ttggttacta aagaataggg gctgtttaac gttacggcgc atcgacccgg
     5041 atttgatttt gatcgaaggg gcacgcgcga cgcccaagcg acgcccaagc gttatttggg
     5101 agacatcccg atcgtggcat gtcgggccag ccggtgatta gcacgcgggc acgcagcgcc
     5161 gatctggcat gtttgccggc acggtcaata aattgaatta catcttggta acgtcaataa
     5221 cgatcaagaa acctgaagat ctcaaaggca gcccgatcgg cgcagcccaa gttggttgaa
     5281 agctttggag cctggatgcc cggcgcgccc gcatcacgat ccttcaattg gacaattaag
     5341 ccgcgcgggg cggttcgtag agcattcggc cagcgatagc tacagcctgg tcccgcgcaa
     5401 caaggaaagg tcaagcaagt catcgccaaa tcgatcccgg tagtggccaa tttcgatagt
     5461 gaaaaaactc aaccacagcg gagttattta gcgaagcgat gagacggcgg tcgatgcaga
     5521 attcagcggg gttggaagtg cagggcgcta tgccagggga caatgcgaaa atccgatgag
     5581 cgtaaagttt acataatata tcttatcaga ctcta
//
LOCUS       DASQKS010000450         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2779076, whole genome shotgun sequence.
ACCESSION   DASQKS010000450 DASQKS010000000
VERSION     DASQKS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2779076"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..736
                     /locus_tag="VE170_11615"
     CDS             536..736
                     /locus_tag="VE170_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16129.1"
                     /translation="MGFLSDEELKKLLPAETAAFPAPIPTQSVSSDEFMPVPQTEKQR
                     EFEARIKAYGSELARHQGLSRH"
     gene            791..1474
                     /locus_tag="VE170_11620"
     CDS             791..1474
                     /locus_tag="VE170_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYJ16130.1"
                     /translation="MPGQLNILSAGAVSPGLVRVIDAFRRETGDDVIVSFATAPELRK
                     RLASGESADMVIAPPELLDELTRSGRAAAEHVMVGRIGVGVMVRDDAPLPGIATVEEL
                     KQSLLNSESIVFNQASTGLYIDSLFKRLGIAAQTEAKIIRCGDFTAVRDHISQGQSNA
                     IGFGATTVIVASSDKGVKFAGPLPSEIQNYTAYAAAVTASAGNHAAAFLDYLATPAAK
                     LILTSVGID"
     gene            1510..2550
                     /locus_tag="VE170_11625"
     CDS             1510..2550
                     /locus_tag="VE170_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16131.1"
                     /translation="MKQLLIVVIVLHLHPIAPNMAQATSHDFYKGKAVRIVVGNSVGG
                     AMDDWARFIALYLGKHIPDSPTVVVQNMPGAGTIIAANHVYNIAKPDGLTLGLVNPAV
                     YIDQLLRATEVKFDWPKFSYIGSPERIDQVLFMRADGPYKTLEDIRKAAEPPRCAAQA
                     RTTPSYFFPKLIEEGLGLKVNIVAGYGGGGEMNLAIEKGEAHCRSGTVSAYVGREPTR
                     TWIKSGFVRALVQSGATRYPKLPETPTIYELLEAHKASDTSKRVTKVMLSSGDLGRPF
                     FAPPGTPADRVKVLRAAFVKTMNDPELLAEAKRRGWDIDLTTGDELEKLAKEVMVQPP
                     EVIERVKKMLAN"
     gene            2562..3053
                     /gene="rimI"
                     /locus_tag="VE170_11630"
     CDS             2562..3053
                     /gene="rimI"
                     /locus_tag="VE170_11630"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYJ16132.1"
                     /translation="MINSELSLRLARASDAAAIANLSRELIEYGLRWRWTAERVAASI
                     SADNTNVLVARVDERVVGFAIMSYGVHVAHLNLFAVASEYQRTGVGSQLLRWLEKCAT
                     VAGIVTIVLEVRAANTGAQRFYESMGYRILVQLPGYYQGTEAALRMGRRLTSLDIKGK
                     KTL"
     gene            complement(3109..>3634)
                     /locus_tag="VE170_11635"
     CDS             complement(3109..>3634)
                     /locus_tag="VE170_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16133.1"
                     /translation="VSFIQAGGAPETLLALVAQGIDAAVLTVPTDAQALKLGYHYVVY
                     GPDLKIAYAATTFITRRSIAAKRGPVIGRFMRAMAEAAKIMHTDKEFTYKVMEKYLRV
                     DDRKLLEASYNVEIKALEPRLAMKPEGFQSTLDEIAPTEPRAKAVKPQEMMDPHYLDE
                     MDKSGFFEQIWGKK"
BASE COUNT          821 a         1030 c         1007 g          776 t
ORIGIN      
        1 gctaaacaca agcaacaaaa ttttccaatt catgaaacct cgcggccact aactcgaaga
       61 cttttcctct tatagcgtaa cgccgcagcg ggctcaacaa aagaaccagt ttcagtggtg
      121 caaatcgttc gagcctttgc aattgttgcc caaggaatcc gaatccggag atatagccgc
      181 atagtatgta agtaggatgc ggttcggttg gttttaggtt tgtagaaatc atgggtcgac
      241 gcggccaagg tcgagtagat aatcttcacg agcatcagcg cgccagagac tagactacag
      301 ccggtctgct tgcctccata tcgatgacct gtccttgaaa tattcggcga cacatccgat
      361 cggtgcggca gccaaaatcg cgattgacat cgctggcagc tctcggctag gtttttgctc
      421 aatcgcagga acagaggtgg cgatttttcc atgccgcatt tttcaaagtc agtccgattg
      481 gcccgttcgt tgtaaggggt ttaatcttta tttctcagtc tcgggagatg ccgttatggg
      541 atttcttagc gatgaagaac tgaaaaagtt attacccgcg gaaaccgccg cttttccggc
      601 gccgatcccg acccagtcgg tgtctagcga cgagttcatg ccggtgccgc aaactgaaaa
      661 gcagcgcgag ttcgaagcgc gtatcaaagc atacggcagc gagctggcac gccatcaagg
      721 cctaagccgg cactaagatt cattgtcggg aagaaaaacg actctcccct gctctaacga
      781 agactccaca atgccagggc aattaaacat tctcagcgcc ggcgcggttt ccccgggtct
      841 ggtgcgggta atcgacgctt tccgccgtga aaccggcgac gatgttatag ttagcttcgc
      901 caccgcgccg gaactgcgca agcggttggc gagcggcgaa tcggcagata tggtgatcgc
      961 gccgccggag ttgctcgacg aactgaccag gtcagggaga gccgccgccg agcatgtcat
     1021 ggtggggcgc atcggcgtcg gcgtcatggt gcgcgacgat gcgcccttac ccgggatcgc
     1081 cactgtcgag gaactcaaac aatcgctttt gaacagcgaa tcgattgttt tcaaccaagc
     1141 ctccacggga ctctacatcg acagcttgtt caagcgcctg ggcattgccg cgcaaaccga
     1201 ggccaagatc atacgttgcg gcgattttac cgccgtgcgc gatcacatca gccagggcca
     1261 gagcaacgca atcggtttcg gcgcgaccac ggtgatcgtc gcgagctctg acaaaggcgt
     1321 aaaattcgcc gggccgctgc cgtcggagat ccaaaactac acggcttatg ccgcggcggt
     1381 gactgcaagc gcgggcaacc acgcggcggc attcctggac tacctggcga caccggcggc
     1441 gaagttaata ttgacatcgg tggggatcga ttgagcgagg gcagtaaatc aaccgcacag
     1501 gagaaagtta tgaagcagct tctgatcgtg gtaatcgttc tgcacctgca cccaatagcg
     1561 ccgaacatgg cgcaggcaac ctcccatgac ttttataaag gcaaggcggt cagaatcgtc
     1621 gtcggcaact cagtcggcgg tgcgatggac gattgggcgc gctttatcgc gctctacctg
     1681 ggcaaacata ttccggactc tccaactgtc gtcgtgcaaa acatgcctgg cgccggcacg
     1741 atcattgccg ccaatcatgt ctacaacatc gccaagcctg acggattgac tctcggactg
     1801 gtcaaccccg ccgtctatat cgatcagctc ctgcgcgcca cggaagtaaa attcgactgg
     1861 ccgaaatttt cctatatcgg ttcgccggaa cggatcgatc aggttttgtt catgcgcgcc
     1921 gacggcccct ataaaacttt agaagatatt cgcaaagcgg ctgagccgcc caggtgtgcc
     1981 gctcaagctc gcaccacgcc tagttatttt tttccaaaac tgatcgagga aggactcggg
     2041 cttaaagtta atattgtcgc cggctacggt ggtggcgggg aaatgaatct ggcgatcgag
     2101 aaaggcgagg cccattgccg ctccggaacc gtatcggctt atgtcggtcg cgaacctacc
     2161 cgcacgtgga tcaagtccgg ttttgtccgc gctctagttc aaagcggcgc gactcgctat
     2221 cctaagttac ccgagacccc gaccatctat gaattattgg aagcacacaa agcatccgat
     2281 acctctaagc gggtcacgaa agtgatgcta tcttccggcg atctgggccg ccccttcttc
     2341 gctccgcctg gcacgccggc cgaccgggtc aaagttttac gcgcagcctt tgtcaaaacc
     2401 atgaacgacc ccgagctact cgcagaagcc aagagacgcg gttgggatat cgatcttacg
     2461 accggcgatg aacttgaaaa actcgccaaa gaagttatgg tccagccgcc agaggtcatc
     2521 gagcgagtaa agaagatgct agcgaactag gacaggagat catgataaac tccgagcttt
     2581 cgttgcgctt ggcgcgcgcg agtgacgcgg cggctatcgc caatctgtcg cgggagctga
     2641 tcgagtacgg cctgcgctgg cgctggacag cggagcgggt ggcagcgagc attagcgccg
     2701 acaataccaa cgttttggtg gcgcgtgtgg acgagcgagt cgtcggcttt gcgatcatga
     2761 gctacggcgt ccacgtcgct catctcaatc tctttgcggt ggcttcggag taccagcgca
     2821 ccggcgtcgg cagccaactg ctgcgatggc tggaaaaatg cgcgaccgta gcgggaattg
     2881 tcacgatcgt tcttgaagtt cgagccgcga acactggcgc gcaacggttc tacgaaagca
     2941 tgggctatcg tatcctcgtc caactgcccg gctattatca aggcaccgaa gcggcactgc
     3001 gcatgggccg gcggctgacg agtttggata tcaaaggcaa gaagactcta taaccagccc
     3061 tagctgctca ttttattcat acgtattcgt caataccgcc tcgagcccct atttcttgcc
     3121 ccaaatctgc tcaaagaagc cgctcttgtc catctcgtcg aggtaatgcg ggtccatcat
     3181 ctcttgcggc ttgaccgcct ttgcccgcgg ttcggtgggc gcgatttcgt ctaacgtcga
     3241 ttgaaaaccc tcgggtttca tcgccagacg cggttccaag gctttgatct cgacgttata
     3301 gctcgcctcc agcaacttgc gatcgtccac ccgcaaatat ttctccataa ccttgtatgt
     3361 gaattcttta tccgtgtgca tgatcttcgc cgcctccgcc atggcgcgca tgaagcggcc
     3421 gatcacaggc ccgcgtttgg cggcaatcga gcggcgggta atgaaagtcg tggcggcgta
     3481 ggcgattttc aagtcgggac cgtagaccac atagtgatac ccaagcttga gcgcctgggc
     3541 atcggtggga accgtcagca ccgccgcatc gattccctgc gcaaccaaag ctaagagcgt
     3601 ctccggcgcg ccgccggcct ggataaaact aact
//
LOCUS       DASQKS010000451         5294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5700124, whole genome shotgun sequence.
ACCESSION   DASQKS010000451 DASQKS010000000
VERSION     DASQKS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5700124"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VE170_11640"
     CDS             complement(<1..244)
                     /locus_tag="VE170_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16134.1"
                     /translation="MALARLAELELYYETQGSGEPVLLAPASWWPSATWNVGVVPFLT
                     GRFETITFDCRGTGRTSQPHNGYSVMQFAQDCLGLLA"
     gene            372..1037
                     /locus_tag="VE170_11645"
     CDS             372..1037
                     /locus_tag="VE170_11645"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ16135.1"
                     /translation="MSNPFVESILKEIIHPGVEQLMSTRFFTELRAGKLSKKRIQGFA
                     VQHYLSNHVINKGLAFSMVRNANNQAMYEQFTELFTEEQSHPGLMRRFGLATGLTDAD
                     FDDGIMIFECVAHTGAIVRGMLVGAPAETRVGALVNETMVQRYSDEFDAALEKHYGLD
                     SRARTFFAVHGKVDVEHTAMAADTVAKFAITDRDKEMVRVAAKNMVRFKLNKFDGIYD
                     TYA"
     gene            1108..2100
                     /locus_tag="VE170_11650"
     CDS             1108..2100
                     /locus_tag="VE170_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16136.1"
                     /translation="MSLTKESLAALWALFCFALGANNALHAAEKSAKLIPLRIAYVSR
                     STLDMPFIIARDRGFFREEGLEPEFIFMKAIQTVQAMLAGGVDFGTATGTAISAAVNG
                     ADVRVVFALSDKPSFDLISAPALTSVAQLRGKKIGISAPGSLTEIIARQILIANKIPL
                     DQVTMLPLGTSDITWVALKAGTIDATMLQIPQNFIAVDEGYRKLAAGADVYRAVQGGL
                     TTTKTVINDKPELVTKVIRATQKALRLFRSDKKYATEFIRGPFLDMGKDRDKYAERVY
                     EAALGYLSTTGTVDDKLQREMIATAAQRTKLAQPPPPERVFDFSFAQKIAETLR"
     gene            2177..3328
                     /locus_tag="VE170_11655"
     CDS             2177..3328
                     /locus_tag="VE170_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ16137.1"
                     /translation="MKYSTDRILTTHAGALPLPPDLKELHNERTAGKSVDEQAFSKRV
                     SGAVADIVKKQIACGLDIINDGEQGKSNFSRYARDRLDGFIEREVKASDHTSTIYARD
                     LTEFNDYFSARAYHRGDNVKRVFCNAPLKYTGHASLKAEIADFKAGLQGQKYADAFLP
                     AVAPGTIEHWMKNDFYANDEAYLAAIADAMHEEYKAIADAGFLLQIDDPDLPDGWQFM
                     SQMTVPEYRKYAELRVEALNHGLRDIPPERVRFHTCWGSYHGPHKYDIPLRDIIDIIL
                     KVNANTISIEAANPRHEHEWRVWQEVKLPAGKVLVPGVVGHATDIVEHPQAIADRLVR
                     YAKIVGKENLMAGTDCGLGPRVGSPQICWAKFEAMAEGARLASKELWGK"
     gene            3473..4498
                     /locus_tag="VE170_11660"
     CDS             3473..4498
                     /locus_tag="VE170_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ16138.1"
                     /translation="MRRGLIYGFIGIALLSVSAGAQAQQLPKSVAIGSNPPGSLFYAL
                     ASGLAKVVSDAAAIQAQVQPYAGTSTFVPLFDTGELEFGVVNAVDMGMVYRGANFKVG
                     GRNPFPHVPSSRLLMRGSPLRSSFIVRKDSPIKTLADVKGKRVTGEYPAQLAVWYNMF
                     GALSNAGLGWNDVKVVPVPAVNEGVDALIQGRADVTTHAMGSAKLKEADAAVGIRFLS
                     LDCSKLGEERIKKAIPGYYLSTVKSGSSSGILEDVCAYTYDIYLVGHAKLPDAVVRSV
                     LQAIWVNIDKLPQFHPGFVEWTKARAVDAEVTIPYHPAAVRYFKDAGVWPAKMDDAQK
                     KLLAWKP"
     gene            4560..>5294
                     /locus_tag="VE170_11665"
     CDS             4560..>5294
                     /locus_tag="VE170_11665"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYJ16139.1"
                     /translation="MIYLHKVATLFFIILLLGVTAPVLAQKLPPSVTIGTNPAGTVFY
                     AVGAGLAKVISGVGPMQSIVQPYTGTSTLLPLLDSGELDFAIINAVESNLAYQGPAKL
                     KIGGKNPLPHVPNARLIMRGSPLFIGLLAKKDGVKSVQEIKGKRVTGEYPANIAIWYH
                     GYAALASAGLTWDDVKVVPVPAVNDGVDAVAQGRADVSIHAIGAAKVKEADAAVGIRH
                     VPVDCSAQGDARVRKAVPGYSTTTLKA"
BASE COUNT         1199 a         1595 c         1455 g         1045 t
ORIGIN      
        1 gcgccaacaa cccgaggcaa tcctgcgcga attgcattac cgagtaaccg ttgtgcggtt
       61 ggctggtccg tccagtaccg cggcagtcga atgtaatggt ctcaaagcgt ccggtcaaaa
      121 acggcacgac gccgacgttc caagtggccg agggccacca ggacgccggg gcaagcagca
      181 ccggctcgcc gctgccctga gtttcgtaat agagttcaag ctcagctaat ctcgccagag
      241 ccatattcct gtcctataga gagctaaggt ttagtagtct gatggttccc tgtcaactga
      301 ttatgtaccg cttgacagcc tatccatccc tctgccactg ttcgcttaca aaatcttata
      361 ggggaatgct tatgtccaac ccatttgtcg aatctatcct caaggaaatt atccacccgg
      421 gcgtggagca gttgatgagc acgcgctttt tcaccgaact gcgcgccggg aaactttcca
      481 agaaacggat acaaggtttt gccgtgcagc attacctgtc gaatcatgtg atcaacaaag
      541 gcttggcttt ctcgatggtg cgcaacgcca acaaccaggc aatgtacgaa cagttcaccg
      601 agttattcac cgaagagcaa tcacatccgg gcctcatgcg acgcttcggc ctggcgactg
      661 ggctgaccga cgccgacttc gacgacggca tcatgatttt cgaatgcgtc gcccacaccg
      721 gagcgatcgt ccgcggtatg ctcgtcggcg cgccggcgga aacccgcgtc ggcgcgctgg
      781 taaacgaaac catggtgcaa cgctattctg acgaattcga tgcggcgttg gaaaaacatt
      841 acggcctcga cagccgggca cgaacgttct tcgcggtcca cggaaaagtc gacgtcgaac
      901 acaccgccat ggcggccgac accgtggcga aattcgccat caccgaccgc gacaaggaaa
      961 tggtccgggt cgcggcgaaa aacatggtgc gcttcaagct caacaagttc gacggcattt
     1021 acgacaccta tgcataagtc ggcttcgagt ttcgggtctt gagtttcgag ttctcttctc
     1081 aaaccatacg gaataggagt gagactcatg agcttgacga aagaaagttt agcggcgcta
     1141 tgggcgctat tttgcttcgc cctaggcgcg aacaatgcgc ttcacgccgc cgaaaaatcc
     1201 gccaagctca tccccctgcg catcgcctat gtctcgcgca gcaccctcga catgccgttc
     1261 atcatcgccc gcgaccgcgg cttctttcgc gaagagggtt tggaaccgga gtttattttc
     1321 atgaaggcga ttcagaccgt ccaggcgatg ctcgccggcg gcgtcgattt tggcaccgcc
     1381 accggcaccg caattagcgc cgcggtcaac ggcgccgatg tccgggtcgt cttcgcgctc
     1441 tcggataagc cgtccttcga tctgatttcc gcgcccgccc tgaccagcgt ggcgcaactg
     1501 cgcggcaaga agatcggcat ctcggcgccg ggatcgctga cagaaatcat cgcgcggcag
     1561 attctcatcg ccaacaaaat cccgctggat caagtcacca tgctgccgct cggcaccagc
     1621 gatatcacct gggttgcttt gaaagccggc accatcgatg ccaccatgct gcaaataccg
     1681 cagaatttca tcgctgtcga cgagggttat cgcaagctcg ccgccggcgc cgatgtctat
     1741 cgcgccgtcc agggcggact gacgacaacc aagacagtta ttaacgacaa accggaactg
     1801 gtgaccaaag tcatccgcgc cacgcaaaaa gcgctccggc tgttccgcag tgacaaaaag
     1861 tacgccaccg agtttatccg cggcccgttc cttgatatgg gcaaggatcg cgataagtat
     1921 gccgaacgcg tctacgaggc cgcgctgggt tacttatcga ccaccggcac cgtcgacgac
     1981 aaactccagc gcgaaatgat cgctaccgcc gcccagcgca ccaagctcgc ccaaccgccg
     2041 cccccggagc gggtcttcga tttcagcttt gcgcaaaaaa tcgccgaaac attacgttga
     2101 caactttttt cccgaatacc tttggaacgg ttccaatcgt tccactcgtt ccatcgcaag
     2161 aaagagggaa ggttaaatga aatacagtac cgatagaata ttaaccaccc acgccggcgc
     2221 cttgccgctg ccgcccgact taaaagagct gcacaacgaa cgcacggcgg gaaaatcagt
     2281 agacgaacaa gcctttagca aacgggtcag cggcgccgtc gctgatatcg tcaagaaaca
     2341 gatcgcctgc ggcctcgaca tcattaacga cggcgaacag ggcaagtcca acttctcgcg
     2401 ctacgcccgc gaccggttgg acggctttat cgagcgcgaa gtcaaagcca gcgaccatac
     2461 ctcgacgatt tacgccaggg atttgaccga gttcaacgac tacttcagcg cgcgcgcgta
     2521 tcaccgcggc gacaacgtca agcgggtatt ttgtaacgcg ccgctgaaat acaccggcca
     2581 tgcgtcgttg aaagctgaaa tcgccgactt caaagccggc ctgcaaggtc agaaatacgc
     2641 agacgccttt cttccggccg tcgcgcccgg aacgatcgag cactggatga agaacgattt
     2701 ctacgccaac gacgaggcct atctggccgc catcgccgat gctatgcacg aggagtataa
     2761 ggccatcgcc gacgccggct tcttgctgca aatcgacgat cccgatttac ccgacggttg
     2821 gcagttcatg tcgcagatga cggtgccgga atatcgcaaa tacgccgagc tgcgagtcga
     2881 agcgttaaat catggcctgc gcgatatccc gcccgagcgc gtgcgctttc atacctgctg
     2941 gggcagctat cacggcccgc acaaatacga catcccgttg cgcgatatca tcgacatcat
     3001 cttgaaggtg aacgccaaca ccatatccat tgaagctgcc aatccacgcc acgaacatga
     3061 gtggcgggtc tggcaggaag tgaaactccc cgccggcaaa gtgctggtcc ccggcgtcgt
     3121 cggccacgcg acggatatcg tcgaacatcc gcaagcgatc gccgatcgat tggtccgcta
     3181 cgcgaaaatt gtcggcaaag aaaacctcat ggccggcacc gactgcggtc tcggcccaag
     3241 agtcggcagc ccgcaaattt gctgggcgaa gttcgaggcg atggcggaag gcgccaggct
     3301 ggcaagcaag gagttgtggg ggaaataatt ccgccaagac gcaggggcga cccgctggtc
     3361 gccctcggaa tattcacacc gaacttttca gtttccccgc tcactctcga atgccattga
     3421 cctagtcacc gtgttcgcat tacagtgacc gccgatttgc cgaggaggat tcatgcgtcg
     3481 aggtttgatt tatggcttca tcgggatcgc cctgctaagc gtcagcgctg gcgcccaagc
     3541 ccagcaacta cccaagtccg tcgccatcgg ctccaatccg ccgggctcgc tgttctatgc
     3601 gttggctagc ggtctggcga aagtcgttag cgatgccgcg gcgattcaag cgcaagtcca
     3661 gccctacgcc ggcaccagca cattcgtgcc attgttcgac accggcgagc tcgaattcgg
     3721 ggtggtcaat gccgtcgata tgggcatggt ttatcgcggc gcgaatttta aagtcggcgg
     3781 ccgcaaccct tttcctcatg tgcccagctc ccggctgctc atgcgcggct cgccgttgcg
     3841 tagcagcttt atcgttagaa aagattcgcc gatcaaaacg ttggccgatg tcaaaggcaa
     3901 gcgcgttacc ggagagtacc cggcgcagtt ggccgtctgg tacaatatgt tcggcgcgct
     3961 ctcgaatgcc gggctcggtt ggaacgatgt caaagtcgtg ccggtgccag ccgtgaacga
     4021 gggtgtcgac gcgctcattc agggccgcgc cgatgtcacc acccacgcca tgggctcggc
     4081 gaaactaaag gaagccgacg cggcggtggg aatccgcttt ttatccctcg actgttccaa
     4141 gctcggcgaa gagcggatca agaaagcgat cccgggatat tatttatcca ccgtcaaaag
     4201 cggctcgtct tctggaattc tcgaagacgt ctgtgcctac acctatgata tttatctggt
     4261 cggccatgca aaactgcccg acgccgtagt acgcagtgtg ctgcaggcga tctgggtcaa
     4321 catcgataag ctgccgcaat ttcacccagg ttttgtcgag tggaccaagg cccgcgccgt
     4381 cgatgccgag gtgacgattc cctatcatcc ggccgcggtg cgctatttca aagacgccgg
     4441 agtctggccg gccaagatgg acgacgccca gaaaaagctt ttggcttgga agccataacg
     4501 actagcgaag tcttggattg atggaaattt gccgtagtgg atctgaacgg agagaagtaa
     4561 tgatttatct gcataaagtc gccacattat ttttcatcat tttgttactc ggtgtaactg
     4621 cgccagtcct cgcgcagaaa ctcccgcctt cggtcacaat cggcaccaat cccgcaggca
     4681 ctgtgttcta cgccgtcggc gccggcttgg cgaaagtgat cagcggcgta gggccgatgc
     4741 aatcgatcgt gcagccttac accggcacca gcacgttatt gccgctgctc gatagcggcg
     4801 aactcgactt tgccatcatc aatgcggtcg aatccaacct cgcctatcaa ggtccggcaa
     4861 agctcaaaat cggcgggaag aatcccctgc cccatgtgcc caacgcccgg ctgatcatgc
     4921 gcggatcacc attattcatc ggcctccttg ccaagaaaga cggcgtaaaa agcgtccagg
     4981 agatcaaagg caagcgtgtt accggcgagt acccggcgaa cattgcgatc tggtatcacg
     5041 gctatgcagc gctggcgagc gcgggactta cctgggacga tgtcaaagtc gtgccagtgc
     5101 cggcagtcaa cgacggcgtc gacgccgtcg ctcagggccg tgccgatgtg agcatccacg
     5161 cgattggcgc ggcaaaggta aaagaagccg acgccgcggt cgggatacgt catgttccgg
     5221 tcgactgctc cgcccaggga gacgcgaggg ttagaaaagc cgtcccgggc tactcgacca
     5281 cgacactgaa agcc
//
LOCUS       DASQKS010000452         7618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5276316, whole genome shotgun sequence.
ACCESSION   DASQKS010000452 DASQKS010000000
VERSION     DASQKS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5276316"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VE170_11670"
     CDS             <1..843
                     /locus_tag="VE170_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018723375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16140.1"
                     /translation="AVQLGAAGAIVYVTGRTTRSQGSDMNRPETIEETAALVDQAGGR
                     GIAVQVDHLIPDQVRALLTRIESEQGGLHILVNDIWGGTKMEWNKSVWESSLDYGLRT
                     LRLAVDTHAITSHFALPLLIKRPGGLVVEVNDGTEEYNATHYRVSFFYDLAKAAVGRM
                     AFALAHELQPHGATALSLTPGWLRSEFMLDEFGVTEATWREATKRVPHFAISESPAFV
                     GRAVAALAQDPDVSRWNGKSLSSGQLAKIYDFTDLDGSQPDAWRYLVEVQDAGKPADV
                     TGYR"
     gene            1002..1376
                     /locus_tag="VE170_11675"
     CDS             1002..1376
                     /locus_tag="VE170_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ16141.1"
                     /translation="MKFLCLSYIDENKFAILSESERDGFVKKCAAYDDTLRKGGHFIR
                     LEALGSAPSAKTLRSRNGKVSVVDGPFAETKEQIGGILLLEAGDLTEATELMAKHPGI
                     QVATFEIRALDEESTARVNPAA"
     gene            1459..1872
                     /locus_tag="VE170_11680"
     CDS             1459..1872
                     /locus_tag="VE170_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16142.1"
                     /translation="MAIEPYLFFNGRCEDAVEFYKKALGAEVLMMMRFKDSPEPPEPG
                     TVAPGSDNKIMHLCMRIGDANIMASDGHCTGQTSFQGFSLSLTAADEADAKRKFAALA
                     EGGQVQMPLAKTFWSPCFGMVADRFGVGWMVTVAP"
     gene            1902..2273
                     /locus_tag="VE170_11685"
     CDS             1902..2273
                     /locus_tag="VE170_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ16143.1"
                     /translation="MKFMLLVHHDEVAFDQIDNAKQQQMLAESIALTHELHAQGQYLG
                     ASPLQPAATAVIVRVHDGETLVTDGPFIETREQIAGYFLIDARDLSEAVSIAAKVPGA
                     RIGPVEVRPLREISGLPDTGA"
     gene            2283..2756
                     /locus_tag="VE170_11690"
     CDS             2283..2756
                     /locus_tag="VE170_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16144.1"
                     /translation="MQKITPCLWFDNNAEEAVNHYLSIFKNSKINKVLRCGDAGPGPK
                     GSVLTIAFQLEGQDFIALNGGPIFKFNEAISLSVDCKSQIEVDDLWEKLSAGGQESRC
                     GWLKDKFGLSWQVVPSILVEMLQDPDPEKAKRVMAAMMKMGKIDIAQLKQAYDRQ"
     gene            2788..3147
                     /locus_tag="VE170_11695"
     CDS             2788..3147
                     /locus_tag="VE170_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HYJ16145.1"
                     /translation="MRYVDGFVLPVPKKNLAAYRRMAQTAGKVWRDHGALEYVECVAD
                     DVKPGKHTSFPQSVKLKPGETVIFAYIVYKSRAQRDRVNAKVMKDPRLAKMMNPKAMP
                     FDGKRMFWGGFNAMIAL"
     gene            3368..3721
                     /locus_tag="VE170_11700"
     CDS             3368..3721
                     /locus_tag="VE170_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16146.1"
                     /translation="MPAPGGTIGHAEIQIDDSHIMLADEYPDMNFRGPKSFGGTSVHI
                     HLYVRDVDRVVKKAVAAGAKVLRPVMDQFYGDRSGSLEDPFGHVWHVATHKEDVPAKE
                     LKKRAAAMAAAAEKK"
     gene            3735..4094
                     /locus_tag="VE170_11705"
     CDS             3735..4094
                     /locus_tag="VE170_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ16147.1"
                     /translation="MKYMLLIYLNEQTPLSEVERQDCYAESAQLAQELQLSGKYLSAN
                     PLHPTAMATSIRVRDGKRLVTDGPFAETREQLGGYFLIDAKDLDEALGVAGRIPMARK
                     GTVEVRPVIEIPGLPQG"
     gene            4154..4561
                     /locus_tag="VE170_11710"
     CDS             4154..4561
                     /locus_tag="VE170_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16148.1"
                     /translation="MNALVNIDVDDLDKAARFYLSAFGLSIGRRLGDFGVEMLGGPAP
                     IYLLARSAGTPASPTTSQRRSYQRHWTPVHLDFVVEDIDPAIQRAVSAGARIETPTIT
                     HNWGKLALMVDPFGHGFCFVQFLGRGYDEIALN"
     gene            4610..4957
                     /locus_tag="VE170_11715"
     CDS             4610..4957
                     /locus_tag="VE170_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ16149.1"
                     /translation="MKYKGSCHCGKITLEVEGELTGAMACNCSMCRRKGSLLWFVPRD
                     QMRLLTPENNMATYTFNKHVIKHRFCPTCGIHPFGEAVDPKGIPTAAINIRCLDGVDL
                     DKVPVQHFDGRSL"
     gene            5003..6190
                     /locus_tag="VE170_11720"
     CDS             5003..6190
                     /locus_tag="VE170_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HYJ16150.1"
                     /translation="MSRVRVLVGTRKGAFVLTSDGKRATWDISGPHFAGWEIYHLKGS
                     RVDPKRIYASQSSGWFGQVIQRSDDGGQTWHQPGTPLGEPTTTPDGEPKGESNKFVYD
                     TSPQTGAPLTTHQWYDGTAHPWEFKRVWHLEPSLTDPDIVYAGVEDAALFRSNDGGQT
                     WQELAGLRGHGSGSLWAPGAGGMCLHTILLDERNPNRIFVAISSAGAFRTDDNGKSWR
                     PINRGLHSLYIPNPTAEIGHCVHRIAMHPARPDVLYMQKHWDVMRSDDAGDTWHEISG
                     NLPTDFGFVIDVHAQEPNTIYVVPIKSDSEHFPPDGKLRVYRSRSGGDEWEALTNGLP
                     QSDCYVNVLRDAMAVDALDECGVYFGTTGGQVYVSADGGDNWQAIVRDLPAVVSVEVQ
                     TLP"
     gene            6187..6483
                     /locus_tag="VE170_11725"
     CDS             6187..6483
                     /locus_tag="VE170_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYJ16151.1"
                     /translation="MIRVTLPAHLAKLAQVNGEVKLDVDGQATLGSVLDALEARYPML
                     RGTIRDHVTYKRRAFVRFYACGEDLSHEAADKRLPDAVAKGTEPFMIIGAIAGG"
     gene            6515..7054
                     /locus_tag="VE170_11730"
     CDS             6515..7054
                     /locus_tag="VE170_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ16152.1"
                     /translation="MLKKILIALAVIVIVLVIVVAFQPAEFRVSRSTVIAAPAPAVFA
                     QVNDFHKWDGWSPWAKLDPNMKTTHAGAPAGTGAIYSWAGNSDVGEGRMTLTESRPSE
                     LVRIKLEFKKPFEATNTTEFSFKPESEKTVVTWSMAGENKFMAKAFGLVMNMDKMVGG
                     DFEKGLAQLKSVAETTGKQ"
     gene            7101..7472
                     /locus_tag="VE170_11735"
     CDS             7101..7472
                     /locus_tag="VE170_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ16153.1"
                     /translation="MNIALWIIQALLAALFLFAGVMKLIMPIEEMTKEIAMPGGFLRF
                     IGVVEVLGGIGLILPWLLRMRPNLTPLAAAGLVIIMIGATVVTLISGATATALIPFVV
                     GLFAAFVAYGRWRLTPLPSKS"
BASE COUNT         1703 a         2159 c         2241 g         1515 t
ORIGIN      
        1 gcggtgcagc tgggagcggc cggagcgatc gtatacgtaa ccgggcgaac gacgcgatcg
       61 cagggctcgg acatgaaccg gccggaaacg atcgaagaga ccgcggctct cgtcgatcaa
      121 gccggcggcc gcggcatcgc agtccaggta gaccatctca ttcccgacca ggtgcgcgca
      181 ttgctcactc gcatcgaaag cgaacagggc ggcctacaca ttctcgttaa cgacatctgg
      241 ggcggcacca agatggaatg gaacaaatcg gtctgggaat cctccttgga ctatgggctg
      301 cggacgcttc gtctggccgt ggacacccac gcgatcacca gccactttgc gctaccgctg
      361 ctgattaaac ggccgggggg cctggtcgtc gaggtgaatg acggaaccga ggaatacaac
      421 gcaacccatt accgggtgtc tttcttttac gacttggcca aggcggcagt aggacgaatg
      481 gcattcgcgc tagcgcacga attacaaccc cacggcgcga cggctctatc ccttactccg
      541 ggttggctgc ggtccgagtt catgctcgat gagttcggcg tcacggaagc cacctggcgt
      601 gaggcgacta agcgcgtgcc gcacttcgcg atatcagaaa gtccggcgtt cgtcggccgg
      661 gcggtggcgg cgcttgcaca agatccggac gtgtcgcgct ggaacggaaa atcgctgtcc
      721 agcgggcaat tggcgaagat ctacgacttt accgatctcg atggcagcca gcctgacgcc
      781 tggagatatc tcgtcgaagt tcaggacgcc ggcaaacccg ccgacgtaac cggctacaga
      841 taaggcgcca gccgacaaag gcatccaagt cgacgcctgc agggtcacac ggagaatatt
      901 ttgcgaagag ctgtcgaatt tacggttggc taatcgatta taggtagagt agcggtttac
      961 gccgcttaat taataaaatt tcccgtacgc ggatcgtcat tatgaaattt ctttgcctct
     1021 catacattga tgaaaataaa ttcgcgattt tgtctgaaag cgagcgcgac ggcttcgtca
     1081 agaaatgtgc cgcttacgac gatacccttc gtaagggcgg tcatttcatt cgtctcgagg
     1141 cactgggaag tgcgcccagt gccaaaacgc tgcgctcccg gaacggcaaa gtgtccgtcg
     1201 ttgacgggcc gttcgctgaa accaaagaac aaataggcgg cattcttttg ctcgaggcag
     1261 gtgatcttac ggaagccacg gagctgatgg ccaagcatcc cggcatccag gtggcgacgt
     1321 tcgagatccg ggctctcgat gaagaatcta cggccagggt caacccggcc gcgtagcagt
     1381 gcaggcgatg gcgtccgctt ccatcgatct acgagttaca cgggcaaacc acaataactt
     1441 gcaaaaggag gaaggtttat ggctatcgaa ccctatctat tcttcaacgg ccgctgcgaa
     1501 gatgcggtcg agttttacaa gaaagcattg ggcgcggaag tgctcatgat gatgcgcttc
     1561 aaagacagcc cggaaccgcc cgagccgggc accgtggcgc ccggctcgga caacaaaatc
     1621 atgcatctgt gcatgcgcat cggcgatgcc aacatcatgg cctccgacgg tcactgcacc
     1681 gggcaaacca gtttccaagg gttctccctg tcgctgacgg ccgccgatga agccgatgcc
     1741 aagcgtaagt tcgcggcgct agccgaaggc gggcaagtgc agatgccgct ggcaaagacg
     1801 ttctggtcgc cctgcttcgg catggtcgcc gaccgcttcg gcgtcggctg gatggttacc
     1861 gtagcgccgt gacgctgggc tgaagagcag gagataggct tatgaaattc atgctgttgg
     1921 tgcaccatga tgaagtcgcc tttgatcaaa ttgacaatgc taagcaacag cagatgctgg
     1981 ctgaatctat tgcgctcacc catgagctcc acgcccaagg tcaatatctc ggcgcatcgc
     2041 cgcttcagcc ggcggcgacg gcggtcatcg tgcgtgtgca tgacggcgag actctcgtca
     2101 ccgacgggcc gttcatcgag acccgtgaac agatcgcggg ctattttttg atcgatgcca
     2161 gggatcttag cgaggcggtg agcatcgccg cgaaagtgcc gggggcacgc atcggtccgg
     2221 tcgaagtgcg gcccttgcgg gaaatttccg ggctaccgga cacaggagcc taaggagacg
     2281 ttatgcaaaa aatcacgccg tgtttatggt tcgataacaa cgctgaagaa gcggtaaacc
     2341 actacctgtc tattttcaag aactcgaaga tcaataaagt cctgcgctgc ggcgatgccg
     2401 ggccgggacc gaagggctcc gtcctcacca tcgccttcca actcgaaggt caggatttca
     2461 tcgctttaaa cggcggcccg atattcaagt ttaacgaagc catatcgctt tcggtggact
     2521 gcaaatcgca aatagaagtt gacgacctgt gggagaagtt atccgccggc ggtcaggaaa
     2581 gccggtgtgg ctggctcaaa gacaaattcg gattgtcgtg gcaggtggtc ccgtccatat
     2641 tggtcgagat gttgcaggac ccggaccctg aaaaagccaa acgggtcatg gccgcgatga
     2701 tgaaaatggg caaaatagac attgcccaac tcaagcaagc gtacgaccga caataaatag
     2761 ttgaaaaaag gaaaaaggag attccctatg cgttatgtcg acggattcgt tctacccgtg
     2821 ccgaagaaaa acctcgcagc ttatcggcgc atggcccaga ccgccggtaa ggtctggcgc
     2881 gaccatggcg cccttgagta cgtagaatgc gtggctgacg atgtgaagcc aggcaaacac
     2941 acgtcatttc cgcagagcgt caagctgaaa cccggcgaaa ccgtaatttt cgcttacatc
     3001 gtctacaagt cgcgcgcaca gcgcgaccgc gtcaacgcca aagtgatgaa agacccgcgc
     3061 ctggcgaaga tgatgaatcc caaagccatg ccgtttgacg gcaagcgcat gttttggggc
     3121 gggttcaatg ccatgatcgc cctgtaaaat gccttagccg ggcgatagcc cgcgaagttt
     3181 aatcgagatc gataaaacgg ctgaagcatt ttacgcaacg ttttttgaca gccgtaaagg
     3241 aagcgtcggt gagaaacacc gtgaaggcta ttcctcgggg ctaccacgcc gtcacaccgt
     3301 atttgagtgt gcggccagcg ccatctcgtt ctacaagaaa gctttcgcag ccaaggagat
     3361 catgcgcatg ccggcgccgg gaggaaccat cggccacgcg gaaatccaga ttgacgattc
     3421 ccatatcatg ctcgccgacg aatatccgga tatgaacttt cgcggaccga aatcgtttgg
     3481 cggcacgtcg gtgcatattc atctctacgt ccgcgacgtc gaccgggtgg taaaaaaagc
     3541 ggtcgccgcc ggcgcgaaag tgctgcgtcc ggtgatggac caattttacg gcgaccgcag
     3601 cggctccttg gaagatccct tcggtcatgt ctggcatgta gcgacccaca aagaagacgt
     3661 cccggcaaaa gaattaaaaa agcgcgcagc ggccatggct gcagcggcgg agaagaagtg
     3721 acagagggct gcccatgaaa tacatgttgc tgatttatct aaacgagcaa actccgttga
     3781 gcgaagttga gcggcaggat tgttacgccg aatccgcgca gctcgcgcaa gagctgcaac
     3841 tgagcggaaa gtatctaagc gccaatcctc tacatccgac agcgatggcc accagtatcc
     3901 gcgtgcgtga cggcaaacgg ttggtgaccg atggcccatt tgccgagacc cgcgaacagt
     3961 tgggcggcta ttttttgatc gacgccaagg atctcgacga agcgcttggc gttgccgggc
     4021 ggattcccat ggcacgcaag ggtacagtcg aggtgcggcc ggtgatcgag atcccggggc
     4081 tgccccaggg ctagtgctgg atagtgaata tcgatagcgt gatcgatttc gccatgaagt
     4141 aaatacccaa gccatgaacg ctcttgtgaa catcgatgtt gacgatctcg acaaagccgc
     4201 gcgcttttac ctatcggcgt ttggcttaag cattggccgt cgccttggtg acttcggcgt
     4261 cgagatgcta ggcggtccgg cgccgattta tctgctcgcc aggtcggctg gaacaccggc
     4321 ctcgccaacg acatcgcagc gtcggagcta ccagcgccat tggacgccgg tccatcttga
     4381 ttttgtcgtt gaagatatcg acccggcgat tcagcgcgcg gtatcagccg gggcgcggat
     4441 agaaacgccg acgattacgc ataattgggg caaactcgct ctcatggtcg acccatttgg
     4501 ccacggcttt tgttttgtcc aattccttgg ccgcggctac gacgaaatcg cgttgaattg
     4561 acgaacatcg agatgaacaa agacatgcaa caatttagga ggttgcccaa tgaagtataa
     4621 aggtagctgt cactgcggca agataaccct tgaagtcgaa ggcgaactca ccggcgcgat
     4681 ggcgtgcaat tgctccatgt gccggcgtaa aggttcgctc ctgtggtttg tgccgcgtga
     4741 tcagatgcgc ttgcttacgc ccgaaaacaa tatggccacc tacacgttca acaagcatgt
     4801 catcaagcat cgcttttgcc cgacctgcgg tattcatccg tttggcgaag ctgtcgatcc
     4861 caaaggcatt ccgacagcgg caattaatat tcgttgtctc gatggagtcg acctcgataa
     4921 agttccggtt caacatttcg acggccggtc gctgtgaagc taagtcgggc cgtagcagcg
     4981 taaattcaca aaggagcaaa ttatgagcag agttcgagtt ttggttggca cccgcaaggg
     5041 cgcgttcgtt ctgacctcgg atggtaagcg cgcaacctgg gacatcagcg ggccgcattt
     5101 cgccggctgg gagatttatc atctcaaggg ttcccgcgtg gaccccaagc gaatctacgc
     5161 atcgcaatcg agtggctggt tcggccaagt cattcaacgc tccgatgacg gcggccaaac
     5221 ctggcatcaa cccggcaccc cgctaggcga accgaccacc accccggacg gcgagcccaa
     5281 gggcgagagc aacaaatttg tctatgacac ctcgccgcaa accggcgccc ccctgaccac
     5341 gcaccaatgg tacgacggca cggcgcaccc ttgggaattc aagcgcgtct ggcaccttga
     5401 accttccctc accgatccgg atatagttta cgccggcgtg gaagatgccg cgttgttccg
     5461 ttccaacgat ggcggtcaaa cctggcagga gttagccgga ctgcgcggtc acggctcggg
     5521 gtcgttgtgg gcgccgggcg ccgggggcat gtgtttgcac acaatcctgc tcgatgaacg
     5581 caacccgaac cggatctttg ttgccatttc atcggctggc gcgttccgca ccgatgataa
     5641 cggcaagagc tggcggccga tcaaccgcgg cctgcattcc ctttatattc ccaatccgac
     5701 ggcggagatc ggccactgcg tgcaccgcat cgccatgcat ccggcacgcc ccgacgtgct
     5761 ctacatgcaa aagcactggg acgtcatgcg cagcgatgac gccggcgata cctggcatga
     5821 gatcagcggc aatctgccca ccgacttcgg attcgtgatc gacgtgcacg cccaggagcc
     5881 caacacgatt tacgtcgtgc cgatcaagag cgactccgag cacttcccgc ccgacggcaa
     5941 gctgcgcgtg taccggagcc gcagcggcgg cgatgagtgg gaagcgctca ccaacggctt
     6001 gccgcaaagc gattgctatg taaacgtctt gcgcgacgcc atggccgtcg atgcactgga
     6061 tgagtgcggc gtgtacttcg gcaccaccgg cgggcaagtt tatgtatcgg ccgacggcgg
     6121 cgacaactgg caggcgatcg tgcgcgatct gccggcggtt gtgtccgtcg aggtgcagac
     6181 gctgccatga tccgtgtcac gctgccggcg catctcgcca aacttgccca agtgaacggc
     6241 gaagtgaaac tcgacgtcga cgggcaggca acgctcggat cggtgctcga cgccttggag
     6301 gcgcgctacc cgatgttgcg gggaacgata cgcgatcacg tcacctataa gcgccgtgcg
     6361 tttgtgcgct tctacgcttg cggcgaggat ctttcccatg aagccgcgga caagcgactc
     6421 cctgacgcgg tggcgaaggg gaccgagcct ttcatgatca tcggcgctat cgccggcggt
     6481 taaagttatt agaaaccgtg ccgggggaat ctctatgctc aagaaaattc tcatcgcgct
     6541 tgccgtaatt gtcatcgtct tggttatcgt cgtagccttc caaccggcag aatttcgcgt
     6601 ctcccgctcg actgtcattg ccgcacccgc cccggcggtg ttcgcgcagg tcaacgactt
     6661 tcataaatgg gatggctggt cgccctgggc gaagctcgat ccgaatatga aaactactca
     6721 cgcaggcgcg cccgccggaa ccggggcgat ctactcgtgg gccggcaata gcgatgtcgg
     6781 cgaagggcgc atgacactga cggagagccg tcccagcgag ctcgtgcgta tcaagctcga
     6841 attcaagaaa ccatttgagg cgaccaacac cacggagttc agcttcaagc ccgaatccga
     6901 gaaaaccgtg gttacttgga gtatggcagg tgaaaacaaa ttcatggcca aggctttcgg
     6961 cctagtcatg aacatggata aaatggtcgg cggcgatttc gagaagggcc tggcgcaatt
     7021 gaaatcagtg gcggaaacta ccggcaaaca gtaacggaag gttgatacgg cgacggcggt
     7081 cgccgccaag gagagccatc atgaacattg ccctgtggat cattcaagcg ttgctcgccg
     7141 cgctgtttct atttgccggc gtcatgaagc tgataatgcc gatcgaggaa atgaccaaag
     7201 agattgccat gcccggcggt tttctccgct tcattggcgt agtggaagtg ctcgggggga
     7261 tcggactgat ccttccctgg ctcctgcgta tgcgcccaaa cctcacaccg ctcgccgccg
     7321 ccggactagt catcatcatg atcggcgcta cggtcgtgac gctgataagc ggcgctaccg
     7381 cgacggcgct gatcccgttc gtggtcggac tctttgcggc gttcgtagcc tatggccgat
     7441 ggcggctgac accgcttcca agcaaatcct gacgctctca acagcgccgc agccgtctca
     7501 ctgcatgccc tcggtaatgt actggatcac gtagccggcg ggatcgtcca tgtaggtgcc
     7561 ggcggtgccg cccgggtggc gacctttggc ttcttcgccg cggtcgccat tggcgacg
//
LOCUS       DASQKS010000453         5593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3185303, whole genome shotgun sequence.
ACCESSION   DASQKS010000453 DASQKS010000000
VERSION     DASQKS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185303"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VE170_11740"
     CDS             complement(<1..184)
                     /locus_tag="VE170_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16154.1"
                     /translation="MKNLLANLEWTRPYFLWLLVLVPLLWLRFGDRRLWVLLARTAIL
                     ALVIFTLADPQMTSQQS"
     gene            complement(181..2001)
                     /locus_tag="VE170_11745"
     CDS             complement(181..2001)
                     /locus_tag="VE170_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HYJ16155.1"
                     /translation="MEFLNLTGLFGFLALPLLLIPYLIRRKPQRLPFSSLLLFMDAGE
                     SASAKPWGRLRLPPLFYLQLLLLALLIFALTEPVFSIRPTNVAMVLDNSASMQALEDG
                     KTRFSLAQERADALAGELGVGAKVDVYLTTPTLEKVHAVPLSPGEAAAIVGKLAPYDL
                     GEAPIDYDTVLEQLARQHKYQRVYLLTDRPARGQSALARVITIGQPHGNTALTAFDVQ
                     RSSLVNARLDASVEVANYSERDEKIKILLKGNGAELAHREVLVGSGKTAAVAFEGIAD
                     HPSYEAEIANPDVLPVDNRRFAVAPASRNLRILGITPRPRELESLKAIPGVQIDIIAP
                     ADYDKSEHSGYGLEIYHFATPAALPRIAALFILPPESSTVVDLGQPVSNVTVSNWRDP
                     HVLTRYVNFNLFRPAYARPLKPQTAGDIIIDSAAGPLAFASERQGVRYLTVGFDPFPY
                     LGRDNLPMSVFTLNLLDWFFDSRGGKSQATGEAIFLGKVQQGDHIVLPGGKKLSITPG
                     AGFFGGAFYQGIYQLTHGGERQLFARNLRDPGESDLRTPAPVAIGGDSPNGASASVLF
                     SFWPYLLIASLALLLLEWFLSPRMAFLKSSRAPARAAQRL"
     gene            complement(2007..2900)
                     /locus_tag="VE170_11750"
     CDS             complement(2007..2900)
                     /locus_tag="VE170_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYJ16156.1"
                     /translation="MLPSPFTPHFLTQLEILRLRTRKEFLGSHTGGYSSPRRGTSLEF
                     ADYRRYTPGDDLRYLDWGIYARTDRLYVKLFREEVDLFAYVFIDASASMGYPSREVKF
                     LPATHVGLALSYVVLANHDHVKLHLLQENSRTNATPFYRGRRRMTDCVNFLALAAPDG
                     SLQLAPVLADHLKKMRRPGKAILISDFLMPAASYQPGLNLLRAFNLDIAVIQILSRHE
                     VDPRFPNGSLALIDSETQKEIRYQWSAEARQDYQAKLAHHNAELRSFCHQSGIHYSLY
                     VTDRDLSDFVFATLPAIGLFK"
     gene            complement(2916..3608)
                     /gene="queC"
                     /locus_tag="VE170_11755"
     CDS             complement(2916..3608)
                     /gene="queC"
                     /locus_tag="VE170_11755"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HYJ16157.1"
                     /translation="MSGTPAVVLLSGGVDSATAAALAQQAGFAIHALSFRYGQRHVCE
                     LDAARRVAHFLNAHSHLVIDFDLRAIGGSALTDDIAVPKSRSATAIAQGIPVTYVPAR
                     NTIFLSFGLALAERIGASDIFFGANQLDYSGYPDCRDEYIRAFETMANLATKAGVEGG
                     SRLTIHAPLLHMTKAAIIAKGIELGVNYGLTWSCYEPGKDGRACGLCDSCQLRVKGFN
                     EAGVIDPIAYVE"
     gene            complement(3756..4088)
                     /locus_tag="VE170_11760"
     CDS             complement(3756..4088)
                     /locus_tag="VE170_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYJ16158.1"
                     /translation="MKRTAWVRLSIWFAPVTLALILLVVPAANADPSKYPEFAQQTLP
                     ADVTPEFLGIDQLVADIKAGAKPVLLDVRTKEEFDEVHILGAQSVPLGEFKTYLKSIP
                     RDRPVVLY"
     gene            complement(4085..4957)
                     /locus_tag="VE170_11765"
     CDS             complement(4085..4957)
                     /locus_tag="VE170_11765"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HYJ16159.1"
                     /translation="MASEKKKPKAKQPAKALLRDGPNWPLFTLAALGMVLSAYLTYTA
                     WAEKLVAFCVQGSGCDVVLNSRWSTLFGMPISFWGFLTYGLLAAVAWNRYADNQWRWA
                     WVISLFGLLFSLYLTAIAFFELKAACPYCLTSLGLMAVIFIMTTLQKPKNLPNFAWGP
                     WLGKSVGVGAAVIVALHLYFAGYWGSAPGPEDPWVRGLAEHLTKTDAKFYGASWCPHC
                     NEQKQLFGSSAKRVPYVECSPGGSQGPLAAICKEKDIKSYPTWIINGQRSTGVQSLDT
                     LAQMSKYTAQGGKP"
     gene            complement(4961..>5593)
                     /locus_tag="VE170_11770"
     CDS             complement(4961..>5593)
                     /locus_tag="VE170_11770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ16160.1"
                     /translation="TRFFLLGAAIIVMGVMLGTMLWWKLGPPTRLLLADGTGDDGLKD
                     YGPVPDFTLTERDGTDMNLARLRGKIWIADFIYTTCTDTCPLQTAAMAELQKEFAANT
                     DVQLVSVSVDPVRDTPKVLSAYADKHAADRRRWYFLTGQRDQVIKLIRDGFHLSVAAL
                     PDSAEQSGMIPHSPRFVLIDRQAQIRGYYDSRAIDGLARLRNDIKTLLKG"
BASE COUNT         1239 a         1541 c         1705 g         1108 t
ORIGIN      
        1 gggattgctg gctcgtcatc tgcggatcgg caagggtgaa gatcaccagc gccaagattg
       61 ccgtgcgcgc cagcaaaacc cacaagcgcc gatcgccgaa gcgcagccaa agcaacggca
      121 ccaaaaccaa aagccagaga aaatacggcc gggtccactc gagattggca aggagatttt
      181 tcataaccgt tgggccgctc gcgctggggc gcgcgaagac ttgaggaaag ccatgcgcgg
      241 acttagaaac cattcgagta aaagtagggc taacgacgcg atcaatagat agggccaaaa
      301 ggaaaaaagc accgacgcgc tggcgccgtt ggggctatcg ccgccgatgg cgacgggcgc
      361 cggcgtacgc agatcggact cgccggggtc gcgcaaatta cgcgcgaaga gctggcgctc
      421 gccgccgtgg gtcagttgat agatgccctg gtagaatgct ccgccaaaaa atcccgctcc
      481 tggagttatc gatagttttt tgccccccgg caggacgatg tgatcgccct gttggacctt
      541 accgagaaaa atcgcctcac cggtcgcttg gctcttgccg ccgcggctgt cgaaaaacca
      601 gtcgagtaga ttcaacgtaa acaccgacat cggcaagttg tcgcggccta agtagggaaa
      661 cgggtcgaag ccgacagtga gataacgcac cccttgccgt tcgctggcga atgccagtgg
      721 accggcggcg ctgtcgataa ttatatcgcc ggcggtttgc ggcttgagcg gccgggcgta
      781 tgccggccgg aacaggttga aattcacata gcgggtcagc acatgggggt cgcgccagtt
      841 ggaaacggtg acgttgctca ccggctggcc gagatcaacc acggtgctgc tttccggcgg
      901 caaaataaac agcgccgcga tcctcggcag cgccgccggg gtggcgaaat ggtaaatctc
      961 caaaccgtag ccggagtgct cggatttgtc ataatcggcg ggcgcgatga tatctatttg
     1021 cacgcctggg atggccttca aactttccag ctcgcggggc cgcggggtaa tccccagtat
     1081 gcgcaggttg cgtgacgccg gggcgacggc gaagcgccgg ttgtcgaccg gcaatacatc
     1141 ggggttggca atctcggctt cgtaactggg atggtcggca ataccctcga aggcaactgc
     1201 cgcggtcttg ccgctgccga cgagaacctc acggtgcgcc aattcggcgc cattgccttt
     1261 tagaagtatt ttgattttct catcgcgctc ggaatagttg gcgacttcca ccgaagcgtc
     1321 caaacgggcg ttcactagcg atgagcgctg tacatcgaaa gcggtcagcg ctgtgttgcc
     1381 gtgcggctgg ccaatcgtga tcactcttgc aagggcgctt tggccgcgcg ccgggcgatc
     1441 ggtaagcaga taaaccctct ggtatttatg ctggcgcgcc agttgctcca gcacagtatc
     1501 gtaatcgatc ggcgcttcac ccaagtcata aggcgcgagt ttgccaacga ttgctgcggc
     1561 ttcgccgggt gagagcggca cggcgtgaac cttctccagc gtcggtgtgg tcagatagac
     1621 gtcgacttta gcaccgacac cgagctcgcc ggccagcgcg tcggcccgct cttgcgccag
     1681 ggaaaaacgc gtcttgccgt cttccagcgc ctgcatactc gcagaattgt ccaaaaccat
     1741 ggctacgttg gtgggccgga tcgaaaacac cggttcggtc aaggcgaaaa ttaacagcgc
     1801 cagcagcaac agctgtaagt aaaatagcgg tggcaatcgg agtcgccccc aaggtttggc
     1861 gctggccgat tcgccggcat ccataaagag cagcaagctc gaaaacggca ggcgctgggg
     1921 tttgcgccgg atcagatacg gaatcagcaa cagcgggagt gccaagaaac caaaaagacc
     1981 ggtaaggttg agaaattcca tggggtttat ttaaacaaac cgatggccgg caacgtggcg
     2041 aagacgaagt cacttaagtc gcgatctgtg acgtagagtg aataatggat gccgctttga
     2101 tggcaaaagc tgcgcagttc agcgttgtga tgggccagct tagcctgata atcctggcgc
     2161 gcctcggcgc tccactgata cctgatttcc ttctgagtct cgctgtcgat caaagccaag
     2221 ctgccgttgg gaaagcgcgg atcgacttca tggcgcgaca ggatttgaat gacggcgata
     2281 tcgaggttga aggcccgcag taaattcaag ccgggctggt aggacgccgc cggcatcaaa
     2341 aaatcagaga tcagaatcgc tttgcctggc cggcgcatct tcttcaaatg gtcggccaac
     2401 acgggtgcca gttggaggga gccgtccggg gctgccaagg cgagaaagtt gacgcagtcg
     2461 gtcatgcgcc gccgaccgcg atagaatggg gtagcattcg tgcggctgtt ttcctgcaac
     2521 aaatgcagct ttacatgatc gtgattggcg agaaccacat agctcaacgc cagaccgaca
     2581 tgggttgccg gcagaaactt cacctcccgc gatgggtaac ccatggacgc actggcgtcg
     2641 atgaagacat aggcgaaaag gtcgacttct tcgcggaaaa gtttcacata gaggcggtcg
     2701 gtgcgcgcgt agatgcccca gtcgagataa cgcaaatcgt ccccgggggt gtaacggcgg
     2761 tagtcggcaa actccaaact ggtgccgcga cgcggcgacg agtaaccacc ggtatggctg
     2821 ccgaggaact ctttgcgtgt gcgcaggcgg agaatttcca gctgggtcaa gaagtgcggt
     2881 gtgaaggggc ttggcagcat gttcaacccg ctagtttact cgacgtaggc gatagggtcg
     2941 ataacgccgg cttcattgaa tcctttgacg cgcagctggc aactatcgca caggccacag
     3001 gcgcggccgt ctttgccggg ttcgtagcaa ctccaagtca ggccgtagtt gacgccgagt
     3061 tcgataccct tggcgatgat cgccgccttg gtcatgtgca acagcggtgc gtggatcgtt
     3121 aaacggcttc cgccctcgac gcccgccttg gtggcgagat tggccatggt ttcaaaggcg
     3181 cggatgtatt cgtcgcggca gtcggggtag ccgctgtagt cgagctgatt agcgccaaag
     3241 aaaatgtcgc tggcgccgat acgttccgcc aaggcgagac caaaggacag aaagatcgtg
     3301 ttgcgtgccg gcacgtaggt gaccgggatg ccctgggcga tggccgtggc gctgcggctt
     3361 ttcggcacgg cgatgtcatc ggtgagggcc gagccgccga tggcacgcag gtcgaaatcg
     3421 ataaccaagt ggctgtgcgc atttaaaaaa tgcgccacgc ggcgcgccgc gtcgagctcg
     3481 caaacgtggc gctggccgta gcggaaactg agcgcatgaa tcgcaaagcc ggcttgttgg
     3541 gcgagcgccg cggcggtcgc cgaatcgacg ccgccgctca gcaagacgac ggcaggtgtg
     3601 ccagacatag attatgagcg cggaactttg cttccttcca ttgggtagcg tttctgatac
     3661 caaccgacaa ggccctcgtt gagcactttc atgttccgat agccgttttc gtggagtatg
     3721 ccataagcca gactggccag gtgatggggg caagctcaat agagtactac cggccgatcg
     3781 cgcggaatgc tttttaaata agttttaaat tcgcccaggg gaaccgattg cgcccctaag
     3841 atatgaacct cgtcgaactc ctctttggta cggacgtcta gaagaaccgg tttagcgccc
     3901 gctttgatgt cggctaccag ttgatcgatg ccgagaaact ccggcgtgac atcggccggc
     3961 agagtttgtt gggcaaattc gggatacttc gacggatcgg cattggccgc tgggacaacc
     4021 agcagtatca aggccagcgt gaccggggcg aaccaaatag ataatcttac ccacgcggtc
     4081 cgcttcatgg ttttcctccc tgggcggtat atttgctcat ttgcgctagt gtatccaagg
     4141 attggacgcc ggtggaccgc tgtccgttaa tgatccaagt gggataactt ttgatatctt
     4201 tctctttgca gattgctgca agcggtccct gggagccgcc tggactgcac tcgacatagg
     4261 gaacacgctt ggccgagctg ccgaatagtt gtttctgctc gttacaatgc ggacaccaag
     4321 acgcgccgta aaacttggca tcggttttcg tcaagtgttc tgccagccct ctgacccagg
     4381 gatcttcggg gccgggagcg ctgccccagt agccggcgaa atagagatgc agcgcgacga
     4441 tcactgcggc gccgacgccg acgctcttac ccaaccatgg accccaggca aagttcggta
     4501 gatttttggg cttttgcaag gtggtcatta taaagatgac cgccataagc ccgagggagg
     4561 tgaggcaata ggggcaggcc gccttgagtt caaagaatgc gatagccgtc aggtagagac
     4621 taaaaagcag tccgaagagc gaaattaccc aggcccagcg ccattggtta tccgcgtagc
     4681 ggttccaggc caccgcggcg agcaggccgt aggttaaaaa tccccagaac gaaatcggca
     4741 tgccgaatag cgtcgaccag cggctgttca agacgacatc gcagccgctg ccttggacgc
     4801 aaaaggcgac gagcttttcg gcccacgcgg tgtaggtgag ataagcggat aagaccatgc
     4861 ccaacgccgc cagggtaaag agcggccaat tggggccatc gcgcaggagc gctttcgccg
     4921 gttgtttagc tttgggtttc ttcttctcgc tcgccatcga tcatcctttt aataaagttt
     4981 taatgtcgtt tctaagccgc gccagcccat cgattgcacg actgtcgtag tagccgcgga
     5041 tctgggcttg ccggtcgatc aagacaaagc gcgggctatg aggaatcatg ccgctctgct
     5101 cggcgctgtc gggaagcgcc gcgacgctca aatggaagcc gtcgcggatc agcttgatga
     5161 cttgatcccg ttgtccggtg agaaaatacc aacgccgccg atcggctgca tgtttgtcgg
     5221 cgtaggcgct cagcactttc ggcgtatccc taaccgggtc gacactgacc gaaactagtt
     5281 ggacgtccgt gttcgccgcg aactctttct gcagctcagc catcgccgcc gtctgcaaag
     5341 gacaggtatc ggtgcaggtc gtgtaaataa aatcggcgat ccaaattttg ccgcgcagcc
     5401 gagccagatt catatcggtg ccatcgcgct cggtcaatgt gaaatccgga accggcccat
     5461 agtccttcaa accgtcatcg ccagtgccat ccgcaagcaa caggcgggtt ggcggcccga
     5521 gtttccacca caacatggtg ccgagcatca cacccataac gatgatcgcg gcgccgagca
     5581 gaaaaaagcg ggt
//
LOCUS       DASQKS010000454         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5700655, whole genome shotgun sequence.
ACCESSION   DASQKS010000454 DASQKS010000000
VERSION     DASQKS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5700655"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VE170_11775"
     CDS             complement(<1..262)
                     /locus_tag="VE170_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16161.1"
                     /translation="MQFVAWGFLVLQALIVVASLLGYGIFTSRPDLLLQFDPQARFFT
                     WAFHGFAVGNMLFGGLAVVADALLRNRRAALVSLVAVYAISLA"
     gene            complement(291..1454)
                     /locus_tag="VE170_11780"
     CDS             complement(291..1454)
                     /locus_tag="VE170_11780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ16162.1"
                     /translation="MKACDVAVVGAGPAGCSAAITLAQRGLQVALIDRAVFPRDKVCG
                     DFLNPINWPVLQELNVAEDLFSVPHTRVSKFRLTVADGSEAVSVFPEQSERQFGLGLR
                     RIHLDHLLVEQAKRAGVDVYEGARLTAVRHQSSGWCLDLNRGGELDSLRPKLLVGADG
                     RNSWVARQLGVASGSPVRSATVGFALQLQNVPGVGGSVEIHQFAGGYAGLVRVDEDTV
                     NLGFAVSRSLLGRSICFETLRANHLRRNQSLDELLSAAEPKSDLRSAWPVYFPPRRCF
                     GDGFLLVGDAARVTEPITGEGVYLALRSGQLAAATIATAIEEGNLSSVRLAAYDRACR
                     EEFGARLRLNSLIRYFVYRPRLLSPAIALLSRRQRLLDSLVSAVCLRRAVDHG"
     gene            complement(1451..2155)
                     /locus_tag="VE170_11785"
     CDS             complement(1451..2155)
                     /locus_tag="VE170_11785"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ16163.1"
                     /translation="MKRSHENEMMDRAGNSPVLLAGELRNLRLLNRYARGSHGVMLAL
                     WRMLGREPLKHLSVLDMGTGSADIPAAILARAKRRRLAANIVGMEADPLTARIAIERI
                     RQHADINIIQGDAGAPPFSPGSFDYVIASQFLHHFTEAKIIDLLKQWAKLARRGIIIS
                     DLVRHPLAYYGIRLLTHLTTRNIMTLTDAPLSVRRSFTFKEWRDLLRQADIGPVEMFS
                     VFPFRMAAVVRLGDPR"
     gene            complement(2152..>3439)
                     /locus_tag="VE170_11790"
     CDS             complement(2152..>3439)
                     /locus_tag="VE170_11790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HYJ16164.1"
                     /translation="NNGLVSNGGPPRRRVVVTGIGVISPNGIGREAYWKALRSGRSGV
                     DLISQFPTEELSCRIAGEVKNFHAEDYLPPQDLRRVGRAVPLLLGATQEALIDAGVVW
                     NQLSLDEKRSWGVIIGSGGGTPDFTEEQYRLFFSNQMRKVSAYNVSSSTPGNLPSEVS
                     LYFDLRGPSHLISTGCTSSTDALGYAFNQIRFGLADHFVTGGADATITPAIMQGFCVM
                     QAVSTSHNNEPARASRPFDRSRDGFVLGEGAWILVLEERELALIRGATIYAEVRGYAS
                     TCDAYHRVRLEPSGEEPARAMSLAIEDARLAKDEVGYIALHGTATVLNDKTETRATKL
                     CFGKRARDLPMSSVKSMIGHPQGASGAAGVVAAICGMNGGFLPPTINLDESDPECDLN
                     YIPNQSIAQDTDIALCNCIGFGSKNSALVIERGATV"
BASE COUNT          810 a         1053 c          959 g          617 t
ORIGIN      
        1 tcgccaaact aatcgcgtag acggcgacca aggagacaag cgccgcccgg cgatttctga
       61 gcaaggcgtc ggccaccacc gcgagtccgc cgaaaagcat attgccgacg gcaaaaccgt
      121 gaaacgccca ggtgaagaaa cgagcttggg gatcgaactg cagtagcaga tcgggacgtg
      181 aagtaaaaat accgtagccc aacaagctcg cgaccacgat cagcgcctgt aatactaaga
      241 agccccacgc tacgaactgc atcgtctatc tccgtccgaa gttgaaaacc tcagccatga
      301 tcaacagcgc gccgcaaaca cacggcgcta accaacgaat ctaggagccg ctggcgacgg
      361 ctcaatagcg caattgccgg cgacaacagt ctcggccggt acacgaaata acgaatcaga
      421 gaattgaggc gaagccgggc gccgaactcc tcccgacaag cgcgatcata ggcagcgagc
      481 cgcaccgagc tcaagttgcc ttcctcgatg gccgtagcga tcgtcgccgc cgcgagctgc
      541 ccgcttctca gtgccaagta aactccctca ccggtgatag gttccgttac acgagcggca
      601 tcccccacca ggagaaaacc atcgccgaaa caacggcgcg gaggaaaata taccggccag
      661 gccgagcgca gatcgctctt cggttcagcg gcgctcagca gctcatcaag gctttgattg
      721 cggcgaagat ggttcgcgcg cagggtctca aagcaaatcg atcggccgag cagcgatcgg
      781 ctgacggcaa agccaagatt caccgtatcc tcgtcaactc gcaccaaacc ggcgtaaccg
      841 ccagcaaact gatgaatttc cacactgccg cctacacctg ggacattttg cagttggagc
      901 gcgaatccca ccgtcgcaga tcggactggc gaacccgaag ccacgccaag ttgtcgcgca
      961 acccaggaat ttcgcccgtc tgcaccgaca agaagcttcg gccgaagaga atctaattcg
     1021 ccgccccgat tcaggtccaa acaccagccc gacgattggt gccgcaccgc cgtcagtcta
     1081 gccccttcgt agacgtccac accggcgcgc ttggcttgct ccactagcag atgatccagg
     1141 tgaatccgtc ttagcccaag gccaaactgg cgttcgcttt gttcagggaa gacactaacc
     1201 gcttcagatc cgtccgctac ggtcaagcga aacttcgaaa ctctggtatg tgggacggaa
     1261 aacaaatctt cggcgacgtt caattcttgc aataccggcc aattaattgg attcaaaaaa
     1321 tcgccgcaca ctttatcgcg tggaaatacc gcgcggtcga tcagcgcgac ctgaaggccg
     1381 cgttgcgcca acgtgatggc ggcagaacat ccggcgggtc cggcgccgac aacggccacg
     1441 tcacacgcct tcatcgggga tctccaagcc gcaccaccgc agccatacga aaggggaaca
     1501 ccgaaaacat ttccaccggt ccaatgtcgg cttggcgcaa taagtcacgc cactctttga
     1561 acgtaaacga acgccgcacc gacagcggtg cgtcggtgag tgtcatgatg ttacgggtgg
     1621 taaggtgagt cagcaaacga atgccataat aagcaagtgg atgtcgcacc aggtcgctga
     1681 tgatgatacc gcggcgggca agcttggccc attgctttaa caaatcgatt attttcgctt
     1741 cagtaaagtg atggagaaac tgtgatgcga tcacgtaatc gaaagagccg ggcgaaaagg
     1801 gcggcgcgcc ggcgtcgcct tgaatgatgt tgatatcagc gtgctgccgg attcgctcga
     1861 tggcaatgcg cgccgttagc gggtcggctt ccatgccaac gatattggcg gcgagacggc
     1921 gccgctttgc cctggcaagg atcgctgccg gaatatcggc gctaccggtc cccatgtcga
     1981 gtacggaaag atgcttaagc ggttcgcgtc cgagcattcg ccagagcgcc agcatcacgc
     2041 cgtgcgagcc gcgtgcgtat ctgttgagca ggcgtaagtt acgcagctca cccgccagaa
     2101 gcaccgggct attgcccgcg cgatccatca tttcattttc gtgacttcgc ttcacaccgt
     2161 cgctccacgc tcgatgacca atgcggagtt tttggaaccg aaaccaatgc aattacaaag
     2221 cgcgatatcg gtgtcttggg cgatgctctg attgggaatg taattgagat cgcactccgg
     2281 atcggactca tccagattga tcgtcggcgg caaaaagccg ccattcatac cacagatggc
     2341 ggcgacgacg cccgccgcgc ccgatgcccc ctgaggatga ccgatcatcg acttgaccga
     2401 actcatcggc aggtcacggg cgcgtttgcc gaaacacaac ttggtcgcgc gggtctcagt
     2461 cttatcattg agaactgtcg cggtgccgtg cagggcaatg taaccgactt cgtctttggc
     2521 gagccgcgca tcttcgatcg ccaagctcat cgcccgggcg ggctcctcgc cgctcggctc
     2581 gagacggaca cggtgataag cgtcgcaagt cgaggcgtaa ccgcgcacct cggcatagat
     2641 cgtcgcaccg cggatcaagg ccaactcgcg ctcttcgaga accaaaatcc acgcgccctc
     2701 gccgaggaca aagccgtccc ggctccggtc gaacggccgc gacgcccgcg ccggttcgtt
     2761 gttgtgggat gtcgacaccg cttgcatgac gcaaaacccc tgcatgatcg ccggcgtaat
     2821 cgtcgcgtcg gcgccaccgg tgacaaaatg atcggccaag ccgaaacgga tctgattgaa
     2881 agcgtagccg agagcatcgg tggaactggt gcagccggtg gagatcagat ggctcggacc
     2941 gcgcaggtca aaataaagag agacctcgct gggcaagtta cccggggtcg agctcgagac
     3001 attatacgcg ctcactttgc gcatctgatt tgaaaagaac aggcggtatt gttcttcggt
     3061 aaagtccggc gtgccgccgc cgctgccgat gatcacaccc caggaacgct tttcatccag
     3121 actcaattga ttccaaacaa ctcctgcatc gattaatgct tcctgagtag cgccgagcag
     3181 tagcggcacg gctcgcccga cccgacgcaa atcctgcggc ggtagataat cctcggcgtg
     3241 aaagttttta acttcaccgg cgatccggca cgacagctcc tcggtaggga actgcgaaat
     3301 cagatcgacg ccactccggc cggagcgcag agccttccag taagcctcac gaccaatgcc
     3361 gtttgggctg atgacgccga tgcccgtgac cactacacgg cggcgcggcg gaccaccgtt
     3421 ggacacgaga ccgttattg
//
LOCUS       DASQKS010000455         5695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3186802, whole genome shotgun sequence.
ACCESSION   DASQKS010000455 DASQKS010000000
VERSION     DASQKS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186802"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..1031
                     /locus_tag="VE170_11795"
     CDS             375..1031
                     /locus_tag="VE170_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16165.1"
                     /translation="MTLATNRRIPYWVKLLYTAFVAIVVPLYWVTYTPWNFLYFCDIA
                     LLVTAAALWMESPLLISMQAVAIVAPQLLWVVDLLCRVVAGVEITGVTSYMLNESIPL
                     YLRGLSLFHGWLPFLLLWLVSRLGYDRRALVLQSAAAIAILLISYLFAPAPPPSAAAP
                     NHAVNINYVYGFTDKQPQTLMAPWLWLVSLMTINIVAFYIPTHFVLRRVFSPRQWNEY
                     "
     gene            complement(1142..1468)
                     /locus_tag="VE170_11800"
     CDS             complement(1142..1468)
                     /locus_tag="VE170_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ16166.1"
                     /translation="MGSKLYVGGLPYSVTEGRLSELFAAHGTVDSASVISDKFTGQSR
                     GFGFVEMSSSSEAQAAIQSLNGTQFEGRSLTVNEAKPMAKRDNFGGGGGGGGRGGDRD
                     GGRNRW"
     gene            1853..2827
                     /locus_tag="VE170_11805"
     CDS             1853..2827
                     /locus_tag="VE170_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ16167.1"
                     /translation="MITANDLKGIMTMMPAFTKKDGDRIEATDTVDTDELSRTVDKII
                     SDGGCNLLTTTGSFGEFHTLLWDEHKKLIEATVAANKKRVPLFVGCTNLNPREAIRQA
                     KFAQDAGADGVLLGVPFYYQASVDNAVQFYHDVADALPKMGIMIYHNPTHHRVTIPVS
                     AFKKLTEKPNIVAMKDSHRTPLQFVELTNITKGKMSLFVNQTQMYPYYQMGAAGCWSF
                     NVWMGPSPVMTLRDACMAQNWELAKQICLDLEGVNRVGPNIGNLVWRENVFKLAVNEA
                     DYCTAGPLRAPWRIVPDEVKDNSKKIAAYWKSLCAKYPMSKEQMKKSA"
     gene            3012..4100
                     /locus_tag="VE170_11810"
     CDS             3012..4100
                     /locus_tag="VE170_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16168.1"
                     /translation="MSTEQKIIDGDGHVVEDIASIVKYMPQEYVARSFSEARGKNPFP
                     PIDHLHSANRHITPPGAFANVGREGWELFLEDVGIGTTVLYTSAGLAFGKIVSRDWAI
                     ELARAYNNWLHDTYLSKSPRFKAMGLIPLQDPTEAVIELRRIVRDLGFSGAMLPSMGV
                     NMPHLGSQKYWPIYGEAERLGCAIAIHGGAHENLLMDDMSPYAVPNALGHPMSQMIAF
                     AGIIFNGVFDKFPGLRIGFMEAGCAWLLTCMERFTGSWASHVQHDPRGRFLQLKKGEK
                     IIDYINRHIDEGRIFVGCEGEELSISEAVRITGNKPYLFSTDYPHEVDAQTCKHELEE
                     LRENPNLKPADKDAILFGNSKRFYQLTN"
     gene            4229..5320
                     /locus_tag="VE170_11815"
     CDS             4229..5320
                     /locus_tag="VE170_11815"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16169.1"
                     /translation="MSNKKHKIIDGDGHVVEDHSAIAGRMPEIYRDRFGSSRSPYPPN
                     DHLHSANAHTLPAGAFAKVGRQGWIDFMDDVGLTSTVLYPTNGLSFGRVVSLDWAIEL
                     AHAYNDWMHDEYLSKSSRFQALSLIPLQEPPEAVKELRRSVKELGFCGAMLPSTGVAG
                     AQNHLGDEKYWPIYEEANRLGCCIGIHGGVHDNMGLDDMTPYAPVNALGHPFGQMVNF
                     SGILFNGIFDKYPKVRFGFMEAGAGWFVNCIERLERAWNSHVQYDPRGRFLKLREKES
                     VTDYIKRHVDEDRIFVGVEGDELTLPFAVSVVGNKPFVFSSDFPHEVNNQTCKEELDE
                     LDENPRLTAEDKDGVRFRNAERFYGLPAN"
     gene            5461..>5695
                     /locus_tag="VE170_11820"
     CDS             5461..>5695
                     /locus_tag="VE170_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16170.1"
                     /translation="MKNAAQTILLLGTLVISFFSTTAINAQPLTNFRIANGTSGENPA
                     VLWVGVELGFYRKHGLNVEVIYMRVGSLAASALV"
BASE COUNT         1352 a         1624 c         1514 g         1205 t
ORIGIN      
        1 attccgtgat ggtatggcaa taattggtat cccagattta aagcttgcca tcgtctgaga
       61 catgattcaa cacaaggaac atgcacttat agtatagaat atagactggg ggcgatgcga
      121 acttctgttc aatacgagac aacggcacgg ggtatgccgg ccaattacga tacgacaaca
      181 cgcattccaa tcgttccaga tacatgcaaa acatcattgc agataaaatt taattctgac
      241 ggctaagttc gcttaaccgc gcgggccgac gaatggccgt aagcgcgacg gtttggagat
      301 ccgggcactg tccatcgatt tatgctaact atcctaagga gcgcttgcgt taacggcgcg
      361 cctcgacagg cactatgacc cttgcaacca accggcgcat tccatactgg gtaaagctgc
      421 tctacacagc cttcgtagcg atcgtcgtgc cgctctattg ggtgacctac accccgtgga
      481 actttcttta cttctgcgat atcgcgctgc tggtcaccgc cgccgcgctc tggatggaaa
      541 gtcccctgct catcagtatg caggcggtag cgatcgtggc gccgcagctg ttatgggttg
      601 tcgacctgct atgtcgcgtc gtcgccggcg tggagataac cggcgtaacc agttacatgc
      661 tgaatgagag catcccgcta tatctccgcg ggctttcgct atttcacggc tggttgccgt
      721 tcctcctgct gtggttggtg tcgcgattgg gttacgatcg tcgagcgctg gttctccagt
      781 ccgcagcggc aatcgcgata ctgttgatct cttatctttt tgcaccggca ccgccacctt
      841 ccgccgccgc tccgaaccat gcggtcaata tcaattacgt ctacggcttc actgacaaac
      901 aaccgcaaac tctaatggcg ccttggctgt ggttggtatc tttgatgacg atcaacatcg
      961 tcgcattcta cataccgact catttcgtgt tacgtcgggt ctttagccca cggcagtgga
     1021 atgagtatta agacgataag caaaggcgac agtcggccgc ttagaaatcg aatggaataa
     1081 aaaagggagg cgccccgacc agacacctcc ccaaatctcg attatttatt taacgagaaa
     1141 actaccagcg gttgcggccg ccgtcgcgat caccgccacg gcctccgccg ccgccgccac
     1201 caccgaagtt atcgcgcttg gccatcggct tggcttcatt gacggtcaag ctgcgacctt
     1261 cgaattgcgt gccattgagc gactgaatgg cggcttgcgc ctcgctgctt gacgacattt
     1321 cgacaaagcc gaaaccgcgc gattgcccgg taaacttatc cgagatcacg ctggcggaat
     1381 ccaccgtgcc atgtgccgcg aaaagttccg ataaacgccc ttcagtgact gaataaggca
     1441 aaccaccgac gtataattta ctacccatgt tgtcctccta gagacagagt tgaggtcttt
     1501 gatcctgaga atctaactgc taaaggggca gaacctgggc tcgtaaaaac taaaaccccg
     1561 ctctggtcgg attgtcattt agaaattgct atcagaggat agagacggaa agaggccttc
     1621 aaattttgct gtcctctcag aaggccattg actgaagaag atgattcaag gtacgggtta
     1681 cacccggtga agtcaaccac acatcacgaa ataaaattat tagatcctaa agctatgttg
     1741 ccgcagaagg aaacggcggc gccacggtat agagccgcgg caattgcgct cgcgccttga
     1801 ctctttcggt cgcctattag acaatccgcc aaaatccagc aaggagattt gcatgattac
     1861 tgccaacgat ctcaagggta tcatgaccat gatgccggca tttactaaga aagatggcga
     1921 ccggatcgaa gccacagaca ccgtggacac cgatgaattg agccgcaccg tcgataaaat
     1981 aatcagcgat ggcgggtgca acctgctgac cacgacgggc agctttggcg agtttcacac
     2041 cttgctttgg gacgagcaca agaagctgat cgaagctacg gtagcggcga ataagaagcg
     2101 ggtgccgtta tttgtaggct gtacgaattt gaatccacgc gaggcgattc gccaggcaaa
     2161 gttcgcccag gacgctggcg ctgacggggt gctgctcggt gtccccttct actatcaggc
     2221 gagtgtcgat aacgcggtgc agttttatca cgatgtcgcc gacgcgttgc cgaagatggg
     2281 cataatgatc tatcacaatc cgacccatca ccgggtgacg ataccggtga gcgcgtttaa
     2341 aaaactcacc gagaaaccca acatcgtcgc catgaaggac agccaccgca cgccgctgca
     2401 attcgtcgag ctgaccaata tcaccaaggg caagatgagc ctcttcgtca accagaccca
     2461 gatgtatcct tactaccaga tgggcgcggc aggctgctgg tcgttcaacg tctggatggg
     2521 accgtcaccg gtgatgacgt tgcgcgatgc ttgcatggcg cagaactggg agctcgccaa
     2581 gcagatctgc ctcgatctcg agggcgtcaa ccgcgtcggc cccaacatcg gcaatctcgt
     2641 ctggcgcgag aacgtcttca aactcgccgt caacgaagcc gactactgca ccgccgggcc
     2701 gctgcgcgcg ccgtggagaa tcgtcccgga tgaagtcaaa gacaacagca agaagatcgc
     2761 ggcctattgg aagagcttgt gcgccaagta tccgatgagc aaagaacaga tgaaaaagag
     2821 cgcataggac gatttaggct ccagcttttc aatagacaaa tgtcagagca gaggcctctc
     2881 ggtgcacatc gaggggcttc tatttttgtg gcgctactga ccaagcgaga tcttcggtgc
     2941 cagctggacc ttcggcctta tttcgttata aaagtatcca ttgcagcaaa acccaacgtt
     3001 agggagaaat catgagtaca gaacagaaaa tcatcgacgg cgacggccat gtggtcgagg
     3061 acatcgcgtc gatcgtcaag tacatgccgc aggagtatgt cgcccggagc ttttccgaag
     3121 cgcgcggcaa gaacccattt ccgcccatcg accatctcca ttcggcgaac cgccacatca
     3181 cgccccccgg cgccttcgcc aacgtcggcc gcgagggctg ggaactgttt ctcgaagatg
     3241 tcggcatcgg caccaccgtg ctctacacca gcgccggcct cgccttcggt aaaatcgtca
     3301 gccgcgactg ggccatcgag ctcgcgcgcg cttataataa ctggctgcat gacacctatt
     3361 tgtcgaaaag cccacgcttc aaggcgatgg gcttgatccc gctgcaagat ccgacagaag
     3421 cggtcatcga gttgcgccgg atcgtccgcg atctcggctt ttccggcgcc atgctgccct
     3481 ccatgggcgt caacatgccg catttgggtt cgcagaaata ttggccgatc tatggcgagg
     3541 cggaaagact gggctgcgcc atcgccatcc acggcggggc gcatgaaaat cttttgatgg
     3601 acgacatgag cccctacgcg gtgcccaacg ccctcggcca tccaatgagc cagatgatcg
     3661 ctttcgccgg catcatcttc aacggcgtct tcgacaagtt cccgggcctg cgcatcggct
     3721 tcatggaagc cggctgtgct tggctgctca cctgcatgga acgctttacc ggctcctggg
     3781 cgagccatgt gcagcatgac ccgcgcggcc gtttcctgca gttaaagaaa ggcgagaaga
     3841 tcatcgatta tatcaaccgt cacatcgacg aaggacgcat cttcgtcggc tgcgaagggg
     3901 aagaactgag catcagcgaa gccgtgcgta tcaccggcaa caagccctat cttttctcca
     3961 ccgactatcc ccatgaggtc gatgcccaga cctgcaaaca tgagcttgaa gagctgcgcg
     4021 agaaccccaa tcttaaaccg gccgacaagg acgccattct gttcggcaat tcgaagaggt
     4081 tttatcagtt gacgaattga cccgctcgcc gcgagcgggt catccctgag cgccagcgaa
     4141 ggatctcgac cgataagacg agattcttcg tcgctgcgct tctcaaaatg acgatcttgg
     4201 aaagagacag tttttaggag agaaagccat gagcaacaag aagcacaaaa tcatcgacgg
     4261 cgacggccat gtggtggaag atcactcggc gattgccggg cgcatgccgg aaatttatcg
     4321 cgaccgattc ggctcgagcc gcagccccta tccgcccaac gatcacttac actcggccaa
     4381 tgcccacact ttacctgccg gtgccttcgc taaagtcggt cgtcaaggct ggatcgattt
     4441 catggacgac gtcggcttga ccagcacagt gctctacccg accaatggct tgtccttcgg
     4501 ccgcgtcgtc agtctcgact gggcgatcga gttggcgcat gcctacaacg attggatgca
     4561 tgacgaatac ctgagcaaaa gttcgcgttt tcaggccttg agcctgatcc cgctacagga
     4621 accgccggaa gcggtgaaag agctgcgccg cagcgtaaaa gagctgggct tctgcggcgc
     4681 catgctgccc agcaccggtg tcgccggcgc gcagaaccat ctcggcgatg aaaagtattg
     4741 gccgatctat gaagaggcca atcgtctcgg ctgctgcatc ggcatccacg gcggtgttca
     4801 tgacaacatg ggactggacg acatgacgcc ctacgcgccg gtcaatgccc tcggccaccc
     4861 cttcggccag atggtcaact tttccggaat actatttaac ggcatcttcg ataagtatcc
     4921 caaagtccgc ttcggtttca tggaagccgg cgccggctgg ttcgtcaatt gtatcgaacg
     4981 cctggagcgc gcctggaaca gccatgtgca atatgatcca cgcggccggt tcttgaaact
     5041 aagagaaaaa gaatcggtga ccgactacat caagcgccat gtcgacgagg accgcatctt
     5101 cgtcggtgtc gaaggcgacg agctgacctt gccgtttgcc gtcagcgtgg tcggcaacaa
     5161 gccgtttgtt ttctcgtcgg actttccgca cgaggtcaat aaccaaacct gcaaggaaga
     5221 actcgacgaa ctggacgaaa acccgcggct cacggcagaa gataaagacg gcgtgcgttt
     5281 ccgtaacgcc gagcgattct acggcctgcc ggcgaactaa gagaaaccgg cacgttccat
     5341 tcgttcaaaa tgttcaagcc gttcaaaacg tcgacggaaa gatctgcaac cggataacca
     5401 accgcaaaac tctgagctca actcccttcg ggcgagggtt cagattttag gaaattattt
     5461 gtgaaaaatg ccgcgcaaac aattctactt ctcggcacgc tggttatttc gtttttctcg
     5521 accactgcga ttaacgctca acccctaacc aacttccgca tcgccaacgg caccagcggc
     5581 gaaaatcccg cggtgctctg ggtcggcgtc gagctaggtt tttaccgcaa gcacggcttg
     5641 aacgttgagg tcatctacat gcgcgttgga tcgctcgccg catcggcatt ggtgt
//
LOCUS       DASQKS010000456         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2781843, whole genome shotgun sequence.
ACCESSION   DASQKS010000456 DASQKS010000000
VERSION     DASQKS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781843"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1056)
                     /locus_tag="VE170_11825"
     CDS             complement(40..1056)
                     /locus_tag="VE170_11825"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16171.1"
                     /translation="MKPVMARGANRLLGNQRGVALIVVLWIFIFLFVVAFDFSASVRE
                     EAEATHRFSDETQGYYLAVAGFERGLYEFINQARGGNAPADPEKSDLFDGSWREEKLG
                     SGGFRVRLVDEGGKINLNRVNEETLRRVFSNLGVDASVVDILVDSIMDWRDPDELHRT
                     NGAESDYYGSLSPPYSAKNGPLDSVEDLLWIRGMTPALYYGSADDSDPRSERAERIGL
                     REVFTIDSPIVRVNLRTATVQVIHAMTGIPIEKCRSFVEERKRLSDKTLTDLLPLLGV
                     GSGDPMLQSFIFTNPSVVAVDAEGRPTDSRAARRVKGVVRLGGAQGFEILRWVDREIG
                     LSQP"
     gene            complement(1053..1718)
                     /locus_tag="VE170_11830"
     CDS             complement(1053..1718)
                     /locus_tag="VE170_11830"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HYJ16172.1"
                     /translation="MSSKWSAISDDGQARRGFHGARGFTLIEVVLAITIFALMGGVLY
                     GAFSLGHSAVEKSAASFSRNQRTRSIGDLLATYIRSAYPYRGGIQDQAIFFTGEQQSV
                     TFVSSYSHAMGGRGMAKIAIEKEEVDNGRARIRLAETAPVRVSGDASDGGQRHSLVIL
                     EGVRDFQLAFLDAEGEKENWEEFWDGAARRRLPRALRMTFIDADGKEVRWVFPLMLAV
                     LSQ"
     gene            complement(1715..2125)
                     /locus_tag="VE170_11835"
     CDS             complement(1715..2125)
                     /locus_tag="VE170_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYJ16173.1"
                     /translation="MKAFPGRGKDGFTLLEVLIAMTIVGLGVVTLLQVFSLGLRLASR
                     SNVQTDAIAQGARVMDELLARPTLRDGSENGGVAPASRWRTQIQTQRDTEPSLNLGSN
                     WELKEVAVEMTVADGERERRVELKTLRLTRKGNP"
     gene            complement(2122..2589)
                     /locus_tag="VE170_11840"
     CDS             complement(2122..2589)
                     /locus_tag="VE170_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYJ16174.1"
                     /translation="MILPAGKDSRGFSLLELVLVLVVIGISMAIVLPNIGRRFQESEV
                     RSSALGLAAVARQLRSRALVDGVAQQLIVSMPQNIYFVPKVREVHLPEDVRIASIEGG
                     EAMERDTKRFYFFPNGSSLGGEIVIASIANSISYLVRFEPLTGKIEVSRGGQL"
     gene            complement(2564..3001)
                     /gene="gspG"
                     /locus_tag="VE170_11845"
     CDS             complement(2564..3001)
                     /gene="gspG"
                     /locus_tag="VE170_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HYJ16175.1"
                     /translation="MKRLLNQAMKNQAGFTLVELIVVVIIIGLLAGLVVPQFIKQEEK
                     ATAKAAKAQIELFGTALDTFRLDVGRYPNSQEGMAALMQKPGGVDRWDGPYLRKDLPP
                     DPWGKPYVYKSPGDHGVYDIISYGADGVPGGDGNNRDITSWER"
     gene            complement(2998..4218)
                     /locus_tag="VE170_11850"
     CDS             complement(2998..4218)
                     /locus_tag="VE170_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYJ16176.1"
                     /translation="MAFYQYRAADQAGKVVEGVMEADAEQSVVSRLREMGCVPLRIAM
                     PSERAVGATRQQRPLFERRKVSQQQLLQFTRELGTLLHAGLPLDRSLSVLGNLFEGGE
                     FTKVMHSLVEAVRAGKSLAVSMGEHPQIFPRIYVNMIRAGEAGGILESVLRYLTEYLE
                     STTALKEEIKSALIYPIILATAAGTSLIVLFLFVIPKFAALFHDNRKAIPWITKVVID
                     SSQMLSDYGWLVLLVVVAAVAGVILYVKNPEGRSEWDRLCLKAWLIGDLVRKFETARF
                     SRTLSALLRGGVPLLEALGTVQGVVGNRLLARAIGQVQVRVREGKGMARPLSESGLFP
                     VLALNMIAVGEESGKLDGMLTEVASFYDQEVRRSTKRLTSLLEPVLILGMGLIIAVVV
                     ISMLMAIFSINEIQ"
     gene            complement(4308..>4616)
                     /locus_tag="VE170_11855"
     CDS             complement(4308..>4616)
                     /locus_tag="VE170_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HYJ16177.1"
                     /translation="TGLEGVAPEMALANGEPLHTIWEAAGCGVCSDTGYLGRVGVFEL
                     LPVTADICKLINQKADANGIRNLAVQQGMRLLREDGWDKVRQGTTTLAEVVRVTREEG
                     "
BASE COUNT         1068 a         1436 c         1192 g          920 t
ORIGIN      
        1 tctaaaaaag aagttaacta cgccatatta cccgaaagct cacggttgcg atagtccaat
       61 ttcccgatcg acccaacgga gtatctcgaa tccttgggcg ccgccgagcc tgaccacccc
      121 tttgacccgg cgcgccgcgc gggaatcggt tggccggcct tcggcgtcga ccgcgaccac
      181 cgaggggttg gtaaaaatga acgattgcaa catcgggtca ccggatccca cacccaagag
      241 cggcagcaag tcggtcaagg ttttgtccga caatcgtttg cgttcctcga cgaagctgcg
      301 acatttctcg atcggaatac cggtcatggc atgaatcacc tgcacggtgg cggtgcgcag
      361 gttgacccgg acgatgggac tgtcgatggt gaacacctcg cgcaatccga tccgctccgc
      421 gcgctccgac cgtggatcgc tgtcgtcggc agaaccataa taaagcgccg gcgtcatgcc
      481 gcgaatccac aggagatcct cgacgctatc aagggggccg ttcttcgccg aatagggtgg
      541 cgacagtgac ccataataat cgctctcggc gccattggtg cggtgcagct cgtcgggatc
      601 gcgccagtcc ataatcgaat cgacaagaat atccaccacc gaggcgtcca ctccaagatt
      661 ggaaaatacc cggcgcaaag tctcttcgtt aacgcgattg agattgattt taccgccctc
      721 gtcgaccagc cgcacacgaa acccgccgct gccaagtttc tcctcgcgcc aactaccgtc
      781 aaacagatcg ctcttttccg gatcggccgg cgcattgccg cccctggcct gattgatgaa
      841 ctcgtacaag ccgcgctcaa aaccggctac agcaagataa tagccttgcg tctcatcgct
      901 gaaacgatgg gttgcctccg cttcttcacg caccgaggcg ctaaagtcga atgcaacgac
      961 aaaaagaaaa atgaatatcc ataaaaccac gatcagcgcc acaccgcgtt gattgccaag
     1021 taaccgattg gcgcctctgg ccataaccgg cttcattggg acagcaccgc caacatcaag
     1081 gggaataccc agcgcacctc tttgccatcg gcgtcgataa aagtcatgcg tagagcgcgc
     1141 ggcagacggc gacgcgccgc cccgtcccag aattcttccc agttttcttt ttccccctcg
     1201 gcatcgagaa aagcgagctg aaaatcacgg acaccttcga gaatcaccaa actatggcgt
     1261 tgcccgccgt cactggcgtc gccgctaacc cgaaccggcg cagtttccgc cagccgtatc
     1321 cgcgcgcggc cgttgtcgac ctcctccttt tctatggcga tcttggccat gccacgaccg
     1381 cccatagcgt gggaatagga cgatacgaag gtcacactct gttgctcgcc ggtgaaaaaa
     1441 atcgcctgat cttgaatccc cccgcgatag gggtaagccg atcggatata ggtcgctagc
     1501 aaatctccga ttgaacgggt cctttggtta cggctgaaac tcgcagcgga tttttccacc
     1561 gccgagtgtc caagagaaaa agcgccatag agcacgccgc ccatgagggc aaagatagtg
     1621 atcgccaaca ctacttcgat caaagtaaag ccacgggcgc catgaaagcc gcgtcgcgct
     1681 tgaccatcgt cagagatcgc gctccatttc gaactcatgg attgcctttg cgcgttagac
     1741 gcaacgtctt tagttccacc cggcgttctc tctcaccgtc agccacagtc atttcgacgg
     1801 caacttcttt gagctcccag ttgctgccca agtttagcga gggctcggta tctcgctgcg
     1861 tttgaatctg cgtgcgccag cggctggccg gcgccactcc accgttttcc gaaccgtcgc
     1921 gcaaagtcgg gcgagccagc aactcgtcca tgacccgcgc gccttgggcg atcgcgtcgg
     1981 tctgcacgtt gctgcgcgac gccaaacgca agccaaggga aaatacctgc agcaaagtca
     2041 ccacgcctaa accgacgatg gtcatggcga tgagcacttc aagcaacgtg aagccgtctt
     2101 taccgcgacc cgggaaagct ttcacaactg accgccacga ctgacttcga tcttgccggt
     2161 gaggggttcg aaacgaacca gataggaaat cgaattggca atactggcga tgacaatctc
     2221 gccaccgagg ctgctgccgt tgggaaagaa ataaaatcgc ttggtgtcgc gctccatcgc
     2281 ttcgccgcct tcgatggatg cgatgcgcac atcctcgggg agatgaacct cgcgaacctt
     2341 tggcacgaaa taaatgtttt gcggcatgct gacgatcaac tgctgggcga cgccatcgac
     2401 taaagcccgg ctccgcagct gccgcgccac cgcggccaaa ccgagcgccg aactgcgcac
     2461 ctcgctctcc tgaaagcgcc ggccgatatt tggcaggaca atcgccatgc tgatgccgat
     2521 gaccaccagc accaggacga gttcaaggag cgaaaatccc cggctatctt tcccagctgg
     2581 taatatcacg gttattacca tcgcctccgg ggacaccgtc ggcaccatag gaaataatgt
     2641 cgtagacgcc atgatccccc ggactcttgt agacataagg cttgccccaa gggtccggcg
     2701 gcaaatcttt tctcagatag ggaccatccc agcggtcaac gccgccgggt ttctgcatca
     2761 aagcggccat gccttcttgg ctattcggat agcgcccgac gtcgagacga aaggtgtcaa
     2821 gcgccgtgcc gaataactcg atctgcgcct tggcggcttt ggccgtggcc ttttcctcct
     2881 gtttaatgaa ttgcggcacc accagcccag ccaacaggcc gataatgata accacgacaa
     2941 tcagttcgac gagggtaaat ccggcttggt tcttcatagc ttggttaagt agacgtttca
     3001 ttgtatttca ttgatgctga atattgccat caacatggag atgacgacca cggcgatgat
     3061 cagtcccatg cccaatatca ataccggttc cagcaacgaa gttaatcttt tcgtcgatcg
     3121 tcggacctct tggtcataga acgacgccac ctcggtcaac ataccatcca acttccccga
     3181 ctcctcgccg acagcgatca tgttcaaagc caagaccggg aataaaccgc tctcgctcaa
     3241 cggccgcgcc atgcccttgc cctcgcgcac ccgtacctgc acttggccga tggctcgcgc
     3301 caagagccgg ttgccgacga cgccttggac cgtacccaag gcttcgagca gcggcacgcc
     3361 gccgcggagc aatgcggata acgtccggga aaaacgcgcg gtctcaaatt ttcgcaccaa
     3421 atcgccaatc aaccaggcct tcaagcataa acgatcccat tcgcttctgc cctcgggatt
     3481 tttcacgtaa agaatcaccc cggcgacggc ggcgacgacc accaacaata ccagccagcc
     3541 atagtctgac agcatctggc tggagtcgat gactactttg gtgatccagg gaatcgcttt
     3601 gcggttgtca tggaacagcg cggcgaattt aggaatgacg aagagaaaca agacgatcaa
     3661 cgaggtgccg gcggcggtgg ctaaaataat cggataaatt aacgccgatt taatctcctc
     3721 tttgagcgca gtagtgcttt ccaagtattc ggttaaatag cgcaggacgc tttccaaaat
     3781 gcccccggct tcaccggcgc gaatcatgtt gacgtaaatc cgcggaaata tctgcgggtg
     3841 ctcgcccatc gacaccgcca gggattttcc ggcgcgcacc gcttcgacca aggagtgcat
     3901 caccttggtg aattccccgc cttcgaatag attaccgagg accgacaaac tgcgatcgag
     3961 cggcaacccg gcgtggagca aggtgccgag ttcacgggtg aactgaagca actgctgctg
     4021 actgaccttg cgtctttcga acagcggccg ctgctggcgg gtagcgccaa ccgcgcgttc
     4081 ggacggcatg gcgatacgca ggggaacaca gcccatctcg cgcaggcgtg ataccacgct
     4141 ctgctcggca tcggcttcca tcacgccctc aaccactttg ccagcttggt cggcggcccg
     4201 atattggtaa aacgccatga agatttttta ggttacgatc agacgactta ggatcaaaaa
     4261 tatatgaccg gtaagcagcc actcccaatc aaatggactg gcgcaaacta tccttcttcg
     4321 cgggtcacgc gcaccacctc ggccaaggta gtcgtcccct ggcgaacctt atcccagccg
     4381 tcctcgcgta aaagccgcat gccctgctgg acggcaagat tgcggatgcc attagcgtcg
     4441 gccttttgat tgatcaattt gcaaatatcc gccgtcacag gtagcagctc gaagacgccg
     4501 acacggccca aataacccgt atcgctgcaa actccacagc cggccgcttc ccagattgta
     4561 tgcaacggct cgccattggc cagcgccatt tcaggggcca cgccctccag tccggt
//
LOCUS       DASQKS010000457         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5702708, whole genome shotgun sequence.
ACCESSION   DASQKS010000457 DASQKS010000000
VERSION     DASQKS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5702708"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VE170_11860"
     CDS             complement(<1..585)
                     /locus_tag="VE170_11860"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HYJ16178.1"
                     /translation="MSQNGAQQFRIEHSTAYRYSEPVMLSHQQLHLTPRRLDYQTTQA
                     HEAVIKPAPTQQRDIIDAFGNPVTEIAIEAAHTSLDIIAKSTVTVIERRLIEPEETPA
                     WETVRDSLAYRASWHPEPAILEATQFLFESPYARVKRTLRAYAGDCFEPGRPILAAAA
                     KLMGKIHGEYKFDAAATNVTTPVMEFFVKKRGVCQ"
     gene            complement(582..3071)
                     /locus_tag="VE170_11865"
     CDS             complement(582..3071)
                     /locus_tag="VE170_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HYJ16179.1"
                     /translation="MNYPLNSDRYDEVFAAQGRPRAHWTRLAQAATRAGRTALSHRAG
                     TIRRAVEQDGVTYNVYGDPKGTDRPWEVDLLPFVISAEEWQFLAKGLAQRARLLNLVL
                     ADLYGANRLLAEGLIPPAIIHGHHNYLWPCRSIEPPGGTFLHLYACDLARSPDGRWWV
                     MGDRTQGPSGAGYAVQNRLIVTPLYENIFRSLGVQRLANFFRTLQQQLAALAPTDGEE
                     PLVALLTAGPYNETYFEHVFLARYLGIPLVEGSDLTVRNNRVYLKTLQGLKRVHGLLR
                     RLDDEYCDPASLRADSALGVAGLISAARAGNVLIANALGSGVIESPALLGFLPAICES
                     LLGERLALPSVATWWCGEALALEHAIDHLPELVIKPVFPSMKLEPTFGHNLDNAKRAA
                     MVERLRATPHAFVAQEWVRLSQAPAWSNESQQFEPRVVGLRLYATAKGDGYEVMPGGL
                     ARVAPESAVEVVTIQRGGSSKDVWVLGDGATPWQSLLAPRLGARNIVRGGFYSPSRAV
                     ENLFWLGRYTERVENVGRLLRAVGQRLAESDPAHLATLKVLTRLSDKARLREAREKAS
                     SVAPATKSPVSLGPEWVIAATGDPQVVNGVPANAARLFFCATQLRERMSLDHWRTVQR
                     LAHVHEPVPQSLEASLAILDRLIPACTALAGYALDDMTRDDAWRFLVIGRDLERMAFL
                     SSAAMQALGLPDEDSDGVLGALLEIGNVSMTYRARYQRHPELIPVLDLLVLDESNPHS
                     VCFQMAALYNHLKQIWARLGFRPINDPASLLLALREFDLVELEDLRMSRDEPLAALLT
                     ACESCVYGLSDELTQRFFIHAGERPQTSVAA"
BASE COUNT          673 a         1007 c         1049 g          644 t
ORIGIN      
        1 ctggcagacg ccgcgcttct ttacgaaaaa ttccatcacc ggcgtggtca cgttggtcgc
       61 cgccgcgtcg aatttatact cgccgtgaat tttacccatc agcttcgccg ctgccgctag
      121 gatcggccgt cccggttcaa aacaatcgcc ggcataggcc ctgagagtgc gtttcactcg
      181 cgcgtaggga gattcgaaga gaaactgtgt ggcctcgagg atcgccggtt cgggatgcca
      241 cgacgcgcga tatgcgaggc tatcgcgaac ggtctcccag gcaggtgttt cctccggctc
      301 gatcaagcgc ctttcgatga ccgtgacggt ggatttggcg atgatgtcca agctggtgtg
      361 cgccgcttcg atggcgatct cggtcaccgg attgccgaag gcatcgatta tgtcgcgctg
      421 ctgtgtcggc gccggtttga ttacggcttc gtgcgcctgg gtggtttgat agtccaaccg
      481 ccgcggcgtc aggtgcagtt gctggtgcga cagcatcacc ggttccgagt accggtaggc
      541 ggtggagtgt tcgatcctaa actgttgggc gccgttttga ctcatgccgc cacgctcgtc
      601 tgcgggcgct cgccggcgtg aataaaaaag cgttgcgtaa gctcatcgga aaggccgtaa
      661 acgcagcttt cgcaagccgt cagcagcgcg gcgagcggtt catcccggga catgcgcaaa
      721 tcctccaact caaccagatc gaattctctc agcgccagca gcaacgacgc cggatcgttg
      781 atcggccgga atcccagccg tgcccagatt tgtttcagat ggttgtagag cgccgccatc
      841 tgaaaacaga ccgagtgcgg gttcgattcg tcgagcacca acagatcgag caccggtata
      901 agttccgggt ggcgctggta gcgcgctcga taggtcatgc tgacgttgcc gatttccaac
      961 aatgcgccaa gcacgccatc gctgtcttcg tccggcagtc caagcgcctg catcgccgcc
     1021 gagcttaaaa atgccatgcg ctcgagatct cggccgatga ccaggaagcg ccaggcgtca
     1081 tcccgggtca tatcgtcaag ggcgtaaccg gccagggccg tgcaggccgg gattaaccgg
     1141 tcgagaatcg ccaacgaggc ttccagactt tgcggcacag gctcgtgcac atgggcgagc
     1201 cgctgtacgg tgcgccaatg atcgagtgac atgcgttccc gcagctgggt cgcacagaag
     1261 aacaggcgcg ccgcgttggc cgggacaccg ttgacaacct gtggatcgcc ggtcgcggca
     1321 atcacccact cggggcctaa gctgaccggt gattttgttg ccggcgctac cgaactggcc
     1381 ttctctcttg cttcgcgcaa gcgcgctttg tccgataaac gagtaaggac cttgagcgtc
     1441 gccaaatggg cgggatcgct ttcggccagc cgttgaccga cggcgcgcaa caagcgtccg
     1501 acattttcca cacgctccgt gtaccgcccc agccaaaaca gattttccac cgctcgtgac
     1561 ggcgaataaa agccgccgcg gacgatgttg cgggcgccga gccgcggcgc cagcagcgat
     1621 tgccacggcg tggcgccgtc gccgagcacc cagacgtcct tgctcgagcc gccgcgctgt
     1681 atggtaacga cctcgacggc cgattcggga gcgacgcggg cgagaccgcc gggcatgact
     1741 tcatagccat cgcctttggc ggtggcgtat aggcgcaaac cgacaaccct tggttcgaac
     1801 tgctggctct cgttcgacca ggccggcgcc tgggagagcc gcacccattc ctgagcgacg
     1861 aaggcgtggg gcgtggcgcg cagccgctcc accatggctg cgcgcttggc gttatcgagg
     1921 ttgtgtccga aagtcggttc gagtttcatc gaagggaaga ccggtttgat gaccagttcc
     1981 gggagatgat cgattgcgtg ctcgagcgcc agcgcttcgc cgcaccacca ggtcgccacc
     2041 gacggcaagg ccagccgctc gcccagcagg ctctcgcaaa tggccggcaa aaagccgagc
     2101 agcgccggcg attcgataac gccggagccg agcgcattgg cgatcagcac attgccagcg
     2161 cgcgccgccg aaatcaggcc ggcgacgccg agcgccgagt cggcgcgcaa actggccgga
     2221 tcgcagtact catcgtcgag acggcgcaac agtccgtgga cccgcttcaa tccctgcaat
     2281 gttttcagat aaacacggtt gttgcgcacc gtcagatccg atccttcgac cagcgggata
     2341 ccgagatagc gcgccaaaaa aacatgctcg aaataggttt cgttgtacgg ccccgccgtc
     2401 aatagtgcga ccagcggctc ttcgccatcg gtgggcgcga gggcggcgag ttgttgctgc
     2461 aaggtgcgaa aaaaattagc cagtctttgc acgccgaggg agcggaatat gttttcatac
     2521 aagggcgtga cgatgagccg gttttgcacc gcgtaacccg cccccgaagg gccttgggtg
     2581 cgatcgccca tcacccacca tcggccatcg ggcgagcgcg cgagatcgca ggcgtaaagg
     2641 tgcaggaaag tgccgcccgg cggctcgatg ctgcggcagg gccaaaggta attgtggtga
     2701 ccgtgaataa tcgccggcgg gatcagccct tcggcgagca gccggttagc gccgtacaag
     2761 tcggcgagca cgagattcag caggcgtgcg cgttgggcta agcctttggc gagaaactgc
     2821 cattcctccg ccgaaatcac gaacggcaac aggtccactt cccagggccg gtcggttccc
     2881 ttgggatcgc cgtagacatt gtaggtgacg ccatcctgct cgacggcgcg gcgaatcgta
     2941 ccggcacgat gcgacagtgc cgtgcggccg gcccgcgtgg ccgcttgggc caaccgcgtc
     3001 caatgcgcgc gtggccgacc ttgggccgcg aaaacttcgt cgtaacgatc ggagttgagt
     3061 gggtagttca taaagggttt gccctggtca tgatgcagaa atggcgctgt ctggtccggc
     3121 aaagggctgc accgagcttc tctctgggct cagagattgg caacgggata gttgaaaaca
     3181 ttgaggaatt taccgtaaca agccggtttg gtgaacacaa ggattttacc atgaactacg
     3241 agaacatgat caaagaaatt ggccgtgatc ttttactgta actggtttcg tagaatgcct
     3301 cttgccaagg cggtcgtacc gtcggaattc aatgtggtaa acaccatgat caattttacc
     3361 gagaccaatc ccg
//
LOCUS       DASQKS010000458         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3188546, whole genome shotgun sequence.
ACCESSION   DASQKS010000458 DASQKS010000000
VERSION     DASQKS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188546"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1480
                     /locus_tag="VE170_11870"
     CDS             431..1480
                     /locus_tag="VE170_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16180.1"
                     /translation="MHKITLTMLAAFVSAVGLLAPREAAPQEKFYQDKTIKVIVGFTS
                     GGFYDRWSRLLARYVPKYLPGNPEMIVQNMPGAGGLIAANNLYNVAKPDGLTLGMTSY
                     GMYLDQLVGRKEAQYEVRKFNWIGSPEKSDVLLYMRADAPYKSIADIQKAAAPPKCGS
                     TGTAGTDFILARLLEETLDAKIETVMGYPGGSEVDLAVEKGEVHCRGMTSAPFFGREP
                     FISWRKRNFVNVLMTGGLKRDPRAPETPTIYEIFDQEKTSEESRRVADVILRGGDFGR
                     PWVMPPETPAEIVKTMRAAYANAMVDTALLDEAKKGKLEVDYVAGEELQKLAETMLSH
                     PPAVVARVKNLLQRK"
     gene            1519..2559
                     /locus_tag="VE170_11875"
     CDS             1519..2559
                     /locus_tag="VE170_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16181.1"
                     /translation="MNPQKLSLISIVLASLLSGGAGMAQTPYYAGKTITIVAGTKAGD
                     VYDLYARHFAQFMPKYIAGNPNIIVQNMAGAGSMIAANFVYNVAKPDGLTIGAIFPAL
                     YFDQVVGRSEVKYDWAKFAWLGSPVTSNHLLYMRADSPYKTIHDVVKASAAPKCGATG
                     TSSTAYYIPKLLDETIGTKFDVVLGYQAGQDIDLAVERNELVCRAFTLTAFFAREPFG
                     TWRKNKFVNVLVQTGRKRDARIKDVPTIYELMDQYKTPEAGRNLATLVLAGGDFGRPY
                     VMPPNTPAEHLKIIREAFEKVLTDEAALADAKKKKLEIDPSTHDELEKLAKEVTSQSP
                     EIVAKMKSLLER"
     gene            2596..3636
                     /locus_tag="VE170_11880"
     CDS             2596..3636
                     /locus_tag="VE170_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16182.1"
                     /translation="MKSAVSIALVISLSFLFGAPQGARAEDFYKGKTVRFIVGQAPGG
                     GYDTYVRTIARHINKHIPGQPSIIVENMEGAGSLILANYVYKQASKDGLTVVIFNNSL
                     VVQSALGDPRIKIDFRKLGWVGAPSVGVPVCMVMGFTGLKTLDDVLKTKLPLKMGATR
                     AGSAGYDMPLILNETLGTKFEVISGFSGTATTRVALQKREINAFCSQWESMRVTARAM
                     LDAKGDDKLIPFVMGGRWDDPEVKDLPLLKDVIKDAKKYAIYRAWASQMDFQRPLTVA
                     PGTPKDRLTILRTALAETLKDPELLEDAKKSKLVITAVSGEKTEKLVDEVLSMSPEAK
                     ASLSFLVRKPGK"
     gene            3786..>4093
                     /locus_tag="VE170_11885"
     CDS             3786..>4093
                     /locus_tag="VE170_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16183.1"
                     /translation="MVKTSIVTLLAMVALSMSPMAEVRAQESFYKGQTIRLVVGLAAG
                     GGYDLYSRVIARHLGKHIPGNPTVIVENMTGAGSVIAANYMYRTAKPDGLTIGHILGG
                     L"
BASE COUNT          892 a         1136 c         1223 g          842 t
ORIGIN      
        1 tggcaatggt ctcgtggaca gaccgggcgc ctatccaatg caccgacgca ggcaagtagt
       61 ggttctaccg atggccgatc ccccacgctg aaggctgcgt gggctgtgaa ccttttttgc
      121 ggccggtcga ggacacgccg aaaattgctt tttcacccgg ccaactccag gtctggcgca
      181 acacccagcg acgcgcgcaa ccgattcgcg ggcgcgccga gtgtaaagca tttgctctgt
      241 ttatctcggc gcccgggcct aaactgtaaa gtatctgcac aagcgctccg ccggcccccg
      301 ctcgggcgaa tcagatttgc ggtaacagct tgtctttgtg cggcattttg ccgatggcac
      361 cggcgttgct ctataccgtg tgcggattcg ggggctgtcg gttcgaacca taatgttcag
      421 agaggatttt atgcataaaa tcacccttac catgttggcg gcttttgtta gcgcggtagg
      481 cttactggca ccgcgcgaag cggcgcccca ggagaaattc tatcaggata aaactatcaa
      541 agtgattgtc ggtttcacgt ccggcggttt ttatgaccgc tggtcgcggt tgctggcgcg
      601 ttacgtgccg aaatatctgc cgggaaatcc agagatgatc gtgcagaaca tgccgggtgc
      661 cggcggtctg atcgcggcaa acaatctcta taatgttgcc aagccggatg gtctgactct
      721 cggcatgact tcctacggca tgtacttgga ccagctggtt gggcgcaagg aagcccagta
      781 tgaagtgcgc aagttcaatt ggattggctc gccggaaaag agcgacgttt tgctctacat
      841 gagggccgac gcgccgtaca aatcgatcgc cgacatccag aaggccgccg cgccgcccaa
      901 atgcggctcg accggtacgg ctggcacgga cttcatcttg gcccgcctgc tggaggagac
      961 tctcgacgcc aagattgaga cagtaatggg ttatccgggc ggcagcgagg tcgacctcgc
     1021 ggtggaaaag ggcgaggtgc actgccgcgg catgacctcg gcgccgtttt tcggccgcga
     1081 gccgtttatt tcgtggcgca aaagaaactt cgtcaacgtg ctgatgaccg gcggcttgaa
     1141 acgcgacccg cgcgcgccgg agacgccgac gatctacgaa atttttgacc aggaaaagac
     1201 ttccgaggaa agccgccggg tcgccgatgt gattctacgc ggcggcgatt tcggccgccc
     1261 ctgggtgatg ccgcccgaga cgccggcgga aatcgtcaag acgatgcgcg cggcctacgc
     1321 caatgcgatg gtggacacgg cgctgttgga cgaagccaag aaaggcaaat tggaagttga
     1381 ctatgtcgcc ggtgaagaat tgcagaagct cgccgaaacc atgctgagcc acccgccggc
     1441 ggtggtcgcg cgggtgaaaa acttgttgca aagaaaataa aggcaatttc ggtttaacca
     1501 ctttcgaggg agttatttat gaatccgcag aagctctcgc taatctcaat cgtgctcgcg
     1561 tcgctgttgt cgggcggcgc cggcatggcc cagacgcctt actacgccgg caagacgatc
     1621 acgattgtcg cgggcaccaa agccggggac gtgtacgatc tttacgcgcg ccacttcgcc
     1681 cagttcatgc ccaaatacat tgccggcaat cccaacatca tcgtgcaaaa catggcgggc
     1741 gcaggttcga tgatcgcggc gaattttgtc tacaacgtgg ccaagccgga cggcctgacc
     1801 atcggtgcga tttttccggc gctgtacttc gatcaagtgg tcggccgctc tgaagtgaaa
     1861 tatgactggg ctaagttcgc ttggctcggc agcccggtga cgtcgaacca cctgctctac
     1921 atgcgggcgg attcgccgta caaaacgatt cacgatgttg tcaaggcgtc ggcggcgccg
     1981 aagtgtggcg ccaccggtac gagttccact gcctattaca ttcccaagct gctcgacgaa
     2041 accatcggca ccaagttcga tgtggttctg ggctatcaag cgggccagga catcgacctc
     2101 gcggtggaga gaaacgaact ggtctgccgg gccttcactt tgacggcgtt tttcgcccgc
     2161 gagcccttcg gcacctggcg caagaacaag ttcgttaacg tgctcgtgca aaccggccgc
     2221 aagcgcgatg cgcgcatcaa agacgtgccg acgatctatg agctgatgga tcaatataag
     2281 acgcccgaag ccgggcgtaa cctggcgact ttggttctcg ctggcggcga ttttggcaga
     2341 ccctatgtga tgccgccgaa cactccggcg gaacacctga agattatccg tgaggccttt
     2401 gaaaaagtgc tgacagacga agcggccctc gccgacgcca agaaaaagaa gttagagatc
     2461 gatccgagca cccacgacga gttggaaaaa ctcgccaagg aggtcacgag tcagtcgccg
     2521 gagatcgtcg ccaagatgaa atcgttgttg gagagatagt ccggctcggt gagatggagc
     2581 gtcacgaggt ccattatgaa atcagcagta tccattgcat tggtgatatc gctaagtttt
     2641 ctcttcgggg cgccccaggg ggcgcgggca gaggactttt acaaaggcaa aaccgttcgc
     2701 ttcattgtcg gccaggcccc cggcggcggc tacgatactt acgtgcgcac gatcgcccgc
     2761 catatcaaca agcatatccc tggccagcct tcgatcatag tcgaaaacat ggaaggcgcc
     2821 ggcagcctga tcttggcgaa ctatgtgtac aagcaggcca gcaaggacgg tcttaccgtg
     2881 gtgattttca acaattcgct ggtggttcag agcgccttgg gcgacccgcg catcaagatc
     2941 gatttccgca aactcggctg ggttggcgcg cccagtgtcg gcgtgccggt gtgcatggtc
     3001 atgggtttta ccgggctaaa aactttggac gatgtcctca agaccaagtt gccgttaaag
     3061 atgggcgcca cccgcgccgg ttccgccggc tatgacatgc cgctgattct taacgagact
     3121 ctcggcacca agttcgaagt catcagcgga tttagcggca cggcgaccac gcgagtggca
     3181 ttacaaaaac gcgaaatcaa cgccttctgc tcgcaatggg agtccatgcg cgtgaccgca
     3241 cgcgccatgc tcgacgccaa gggcgacgac aagttgattc cttttgtcat gggcggccgg
     3301 tgggacgatc cggaagtcaa agatctgccg ttgcttaagg acgtcatcaa ggacgcgaaa
     3361 aaatacgcca tctaccgcgc ttgggcgtcg cagatggatt tccaacggcc gctcaccgtt
     3421 gcgcccggta cgccgaaaga ccggctgacg attttgcgca cggccttggc cgagacgctc
     3481 aaagatcccg agctgttgga agacgcgaaa aaatcgaagt tggtgattac cgcggtctct
     3541 ggtgagaaaa ccgaaaagct ggttgacgag gtgttgtcga tgtcgccgga ggccaaagcc
     3601 agtctgtcgt ttctggttcg taagccgggg aagtagcgcc ttcagtttcg cgtttacgct
     3661 cggcgtggcg cttccggcta cgctgttcga gcaggttgct cgtcgctaca gcatcaacgt
     3721 gtagccgcat cagcaaattg tcggagaagt cattgcattg cccaaaaagg ttgaggtcgg
     3781 aaaccatggt taagacaagc atcgttacgt tactggccat ggttgctctg tcgatgtcgc
     3841 cgatggcgga ggtgcgcgcc caggaatcat tctataaagg tcagacgatt cgcctggtcg
     3901 ttggcctcgc tgccggcggc ggttacgatc tgtactcccg agtgatcgcg cgccacctcg
     3961 gaaaacatat tccaggaaat cccacagtaa tcgttgagaa tatgaccggc gcgggcagcg
     4021 tgatcgccgc aaattacatg tacagaaccg ccaagcctga cgggctgacg atcggccaca
     4081 ttctcggtgg cct
//
LOCUS       DASQKS010000459         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2364574, whole genome shotgun sequence.
ACCESSION   DASQKS010000459 DASQKS010000000
VERSION     DASQKS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364574"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..2773
                     /locus_tag="VE170_11890"
     CDS             470..2773
                     /locus_tag="VE170_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYJ16184.1"
                     /translation="MASVADGIAAGIERKFTEIELRQSEERFRELAENINEVFFSISA
                     DGSALHYVSPAYEQVFGRKKETLYQNPHAWLDAVHSDDRRRVGQAEQNHPEAFDEEYR
                     IVRPDGQLRWIHARTFPVKNSAGEIVRRVGFAEDITERKRSEEKVQQNLQRIRALRNI
                     DTAISSTLDLRTVLNILLENIELFLPIAVATTVRLLNDETGKLESLACRGLDEEEWRG
                     QQQPTPAGRAQKIVETRAPVIVRNIVDDQDTYNGDIFRKHGLVSYLGMPLIAKDKPLG
                     VLSLYTNHEHEFDKQEVEFLDTLAGRAAIAIHNAQLHEQTKKQHLALIEQEGIQRVMK
                     ELSQDITTMTVDRLLEKLTATIRQLLNVDIADVHLIGAQKLLDLLVSSDQRVERFGEG
                     GGFNGASQWVINQRRSIAIRDFPAQTEFPLGGVVEKFGIRGFLAAPLTARSGEVIGVI
                     RALSKEPRDFSAQEIDLFEQLANGAAVALVNEQLYTESQKASRAKSEFLCVMSHELRT
                     PLNVIMGYAALVKEDMERAGDERHRAWLETIQAQARELQDMFDAIMQATAIESGSARA
                     NFDQVDCSGLLQDLQETHEHRAKKKVAVIWNYPVDLPPLRTDPAKLRWILHNLIKNAI
                     KFTDQGSVTVSARLRARTNRTNKESAKQKIEETTNGRMGERRNLACLEVSVTDTGIGI
                     AKETLPIIFDIFRQVDSSDTRAYGGAGLGLYIVKKLTEVLGGTVDVQSVLGKGSTFVV
                     SIPCEPLRIVARPADTRTSARQSEGQV"
     gene            complement(2885..>3122)
                     /locus_tag="VE170_11895"
     CDS             complement(2885..>3122)
                     /locus_tag="VE170_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16185.1"
                     /translation="HAVHEAGSISGGRDNTFDFRHRASLCCASKVGSVGSGGLIERRT
                     IKLFSAMAQSPFAFCIPHNTKGQAFTSQQSARKV"
BASE COUNT          775 a          831 c          902 g          614 t
ORIGIN      
        1 tgaaaaattt gccgcggcga acaaaatcct cgaagaacga acaatactag caagttttaa
       61 cgctgaaatc gcccatgcgc tgacacaaag gatagttcta gcggaaatcc tgcagctctg
      121 tacggaagct atggttcgtc acttgaacgc cgccttcgca cgcatctgga cttacaacca
      181 aaacgatcac gtccttgaaa tgcaagctag cgcgggcatg tacacgcacc taaacggcgg
      241 ccatgcgcga gtacctatgg gggagttcaa gatcgggctt atcgcctctg agcaaaggcc
      301 tcatttgacc aactatgtaa tcggcgatcc tcgtgtgaac gatcaggaat gggcgaagcg
      361 cgaaggcatg gtggccttcg ccggttatcc gctcatcgtc gaagaacgac tggttggcgt
      421 aattgcgatg tttgctaaaa acccgctcag tcaaaacacc ttggatgcca tggcatccgt
      481 cgccgacgga attgccgccg gcatcgaacg caaatttacg gagattgaac tgcgacaaag
      541 cgaggagcgt tttcgtgaat tggcggaaaa tatcaatgaa gtgtttttct ccatcagtgc
      601 cgacggtagc gcgttgcact atgtcagtcc ggcctatgag caggtctttg gtaggaaaaa
      661 ggaaacgctc taccagaacc cccacgcgtg gttggacgcc gttcattcgg atgatcggcg
      721 gcgcgtgggg caggctgaac aaaatcatcc ggaggctttt gacgaggaat atcgcatcgt
      781 ccgccccgat ggccaactgc gctggattca cgcgcgcacc ttcccggtga agaacagcgc
      841 cggcgaaata gtacgtcggg tcgggttcgc cgaagatatc accgagcgaa aacgatccga
      901 agaaaaagtt cagcagaatc tccagcgtat ccgggccttg cgcaatatcg ataccgccat
      961 cagctcgacc ttggacctgc gcacggttct taacattttg ctggagaata tcgagctctt
     1021 cttgccaatt gccgtagcca cgacggtgag actcttgaac gacgaaaccg gcaagctgga
     1081 atccctggcg tgtcggggtc tggatgagga ggaatggaga gggcagcaac aaccaacgcc
     1141 ggccggacgg gcccaaaaga ttgtcgaaac cagggctccg gtaatcgtcc gcaatattgt
     1201 tgatgatcaa gatacttaca acggggatat ctttcgtaag cacggacttg tgtcatatct
     1261 aggaatgccg ctgattgcta aggacaaacc tcttggtgtc ctaagtctgt ataccaatca
     1321 tgagcatgaa tttgataaac aagaggtcga gtttctcgat acgctagcgg ggcgggccgc
     1381 tatcgcgatc cataacgccc agcttcatga gcaaacgaaa aaacagcatc ttgccttgat
     1441 cgagcaggaa gggattcagc gcgtcatgaa agagctgagc caggacatca caacgatgac
     1501 cgtcgatcgg ctgcttgaga agcttacggc taccataaga caattgttga acgtcgatat
     1561 cgccgatgtg catttaattg gcgcgcaaaa attgctcgac ttgctagtct caagcgacca
     1621 gcgggtcgag cggttcggcg agggcggcgg cttcaacggc gcgagtcagt gggtcatcaa
     1681 ccagcgccgt tccatcgcga tccgcgactt cccggcgcaa actgaattcc cgctcggagg
     1741 tgtcgtggag aagttcggca tccgcggctt tctcgccgcg cccctgaccg cgcgcagcgg
     1801 cgaagtgatc ggcgtcattc gcgcgctgtc gaaagagccg cgtgatttta gcgcgcaaga
     1861 aatcgatctt ttcgagcaat tggccaacgg cgctgccgtc gccctggtga acgagcagct
     1921 gtacaccgag tcccaaaaag caagccgcgc caagtcggaa tttttgtgcg tcatgtcgca
     1981 cgagctgcgc acgcctttga atgtcatcat gggttacgcc gcgctggtga aggaagatat
     2041 ggaacgcgcg ggcgatgagc gccatcgcgc gtggctggaa acaatccagg cccaggcgcg
     2101 cgagctgcag gatatgttcg acgcgattat gcaggcaacg gccatcgagt ccgggtcggc
     2161 ccgcgccaac ttcgaccaag tcgactgcag cgggctgctg caagatctgc aagagaccca
     2221 cgaacaccgg gccaaaaaaa aggtggccgt gatctggaat tatcctgtcg acctgccgcc
     2281 gctgagaacc gacccggcca agctcagatg gattctccac aatttgatca aaaacgcgat
     2341 caagtttacc gaccaaggca gcgtcaccgt cagcgcgcgg ctgcgcgcac ggacgaatcg
     2401 gacgaacaag gaatcggcga agcaaaaaat tgaagaaacg acgaatggga gaatgggaga
     2461 gcggaggaat ctcgcttgcc tagaggttag cgtcaccgac accggcatcg gtatcgccaa
     2521 agaaactctg ccgattattt tcgacatatt ccgtcaggtg gacagctcgg acacgcgcgc
     2581 ctatggcggc gcgggcttgg gactttacat cgtaaaaaag cttaccgagg tgttgggcgg
     2641 gaccgtagac gtgcagagtg tgctcggcaa gggatcgaca ttcgtcgtgt cgattccctg
     2701 cgagccgtta cgcatcgtcg cgcgcccggc agacactcga acgtcggcaa gacaaagcga
     2761 ggggcaagtc taagaagctt ggccaagcaa tcgaacaact cctagacaag gctagggtga
     2821 accgatgata agccatactc tcagatcggt cccggggtga gggtccgaac tctgaccttt
     2881 atttctagac tttccttgca gattgttgcg acgtaaaagc ctggcccttt gtgttgtgcg
     2941 ggatgcagaa ggcgaacgga gattgtgcca tggccgagaa taacttaatt gtgcggcgct
     3001 cgataaggcc gccggaaccg acgctgccaa ctttagaggc gcagcacaag gaggctctgt
     3061 gacggaagtc gaacgtattg tcccgcccgc cggaaataga gcctgcctca tgaaccgcgt
     3121 ga
//
LOCUS       DASQKS010000460         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_233445, whole genome shotgun sequence.
ACCESSION   DASQKS010000460 DASQKS010000000
VERSION     DASQKS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233445"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VE170_11900"
     CDS             <1..768
                     /locus_tag="VE170_11900"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16186.1"
                     /translation="VAISFTAAGATVAANLQGADVVLLGATIDSLPFELWSVPSISAP
                     EQLKGSKLGVSRIGATTDFVARYLLKKWNLQPDKDVAIFQTGSGPQIFAAIKGGSVQS
                     GVLATGPETISAEAAGFVRLADVSTSGLVYPFGPFAARQAFLKSQPDLVTRFMKAYVE
                     GIHRFKADKTAALAVLEKYTKQKTTPAAEKVYEIYANKYFKRVPEATPAGIQTIIEEL
                     SGSRAIPPGTMPARFAESRFIRDLAANGFVDALYKSR"
     gene            804..1427
                     /locus_tag="VE170_11905"
     CDS             804..1427
                     /locus_tag="VE170_11905"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HYJ16187.1"
                     /translation="MKTILQNHVPHNTGWSAAMKQGQVIRITATTTVDFVFFKLQNLI
                     ERFDQPRTKVYNMKLFISTGDKLMGRNNQHMMTIVYDGNQVGTHDLQKGMCSGYRFKL
                     AQQENRLAEYYPREIKEIPDHGCYENLSKAVASYGIIPEDIPSPFNLNQHMIIDGKTG
                     KMEHTQVRPPEGNYMDLRAEMDLLVALSACPDLAVGGKPVDVMIYEE"
     gene            1565..2101
                     /locus_tag="VE170_11910"
     CDS             1565..2101
                     /locus_tag="VE170_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16188.1"
                     /translation="MPYGVLDPQKLSQASRSCVERALDWWQKSMQTTKHKTYLVLAGY
                     DSDQGQEIKLRRELVHSSVQDPELIGNIVEVSASNEVGLTQKISRIRTLLPVETITVF
                     VESRNAISVKAIFQRKFGKTLQIRKFKANFEFNHQWITTSTSIAWSARNWFLRLWFEL
                     KKRMGRGLRKKIRYWFRG"
     gene            complement(2144..3001)
                     /locus_tag="VE170_11915"
     CDS             complement(2144..3001)
                     /locus_tag="VE170_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ16189.1"
                     /translation="MSLEEMHSNGKIHWPGDARIAVLLSFDFQGGEDVRPDKNGKINH
                     EEYTQAEYGPNTGIWRILRILAAHEVRATFLTCGGIAERYPDAVTAIIAAGHEIAGHG
                     YHHEVARDLSKDEERDVMKRTTDMILNRAQKRPVGWRSCTQSPNSIELLMEQGYLWNS
                     NSFSHDLPFIWQNGKKKLVELPRQPFGDGRTYGHRDSGNPNDTLVVWKAMFDQFYEES
                     SLAPTFCPFQFHPYISSRAGRAKTLSDIIGHMKSHKGVWFATGSEVAHWWLEKGFSAE
                     VNGKISKVA"
     gene            3196..4413
                     /locus_tag="VE170_11920"
     CDS             3196..4413
                     /locus_tag="VE170_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019665239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16190.1"
                     /translation="MAEGKFNVGGVVLERPFKIRRLGHFGFNLANMEAGRHFYVDLLG
                     FKVSDVMDYSLRAKSPEDVAGLGDPNGYFTRYGGDHHAMVLFPKRVRDALGRSEAPGV
                     TINQITWQVGSMREVGMAIQWFNEHGIKQQRSGRDMPGSNWHTYLLDPDGHSNELYYG
                     IEQIGWDGYSKPKSMYDRGFNTPPELPQISEFDEVEQAKANGIDLTSGYHHVDDLPAS
                     FDVDGILLPRPFKIVRLGPVYLFADDMAAAEKFYRQRMGFTLTEEVNWQGHRCLFFRC
                     NTEHHSLALFPTAARAALGLSGHSKTAALGLQLANYRQLKDAVAFLRSQGVKVSEAIP
                     SELHPGIDLAAHATDPDGHAIQLYCAMEQIGWDGKPRPKELRQPKPLSDWPATLEANS
                     DVYAGEPFLGPWG"
BASE COUNT         1012 a         1358 c         1260 g          936 t
ORIGIN      
        1 gtggcgatct cgtttaccgc cgccggcgcc acggtagctg ccaatctcca gggcgcggat
       61 gtggtcctgc tcggcgcgac catcgactcg ctgccattcg aactgtggtc ggtgccgagc
      121 atcagcgcac cggagcaact aaaaggcagc aagctcggcg tcagccgcat cggcgcgacc
      181 accgatttcg tcgcccgcta tttgctcaag aaatggaatt tgcagccgga taaggacgtg
      241 gcaatttttc aaaccggttc gggaccgcag atcttcgccg cgattaaagg cggctcggtg
      301 caaagcggcg tactcgccac cgggccggaa accatcagcg ccgaagctgc cggcttcgtg
      361 cgcttggccg atgtctccac ctcggggctg gtctacccct tcggcccgtt cgcggcgcgc
      421 caggcgttct tgaagagcca gcccgacttg gtaacgcgct tcatgaaagc ttacgtcgag
      481 ggcattcacc gctttaaagc cgacaagacg gccgccttgg cggtgctgga aaaatacacc
      541 aaacaaaaaa ccacgccggc tgcagaaaag gtctacgaaa tttacgcaaa caaatacttt
      601 aagcgggttc ccgaagcgac ccccgcgggt attcaaacca tcatcgaaga gctgtcagga
      661 tcgcgcgcga ttccgcccgg caccatgccg gctcgatttg ccgaatcccg cttcatccgc
      721 gatctggccg ccaacggctt cgtcgacgcg ctctacaagt cgcgataaac gctcggtctc
      781 gaacctcaag gaggacttta gctatgaaaa ccattctgca aaaccatgtg ccgcacaaca
      841 ccggatggtc ggcggcaatg aaacaaggcc aggtgatccg catcacggcg accaccacgg
      901 tggattttgt tttcttcaaa ctgcaaaact tgattgaacg tttcgatcag ccgcgcacca
      961 aagtttacaa catgaagctg ttcatcagca ccggcgacaa gctcatgggg cgcaacaacc
     1021 agcatatgat gacgatcgtc tacgacggca accaagtcgg cacccatgat ttacagaaag
     1081 gcatgtgcag cggctaccgc ttcaagctgg cgcagcagga aaatcgcctg gcggaatatt
     1141 acccgcgcga aatcaaggaa atccccgatc acggctgcta tgaaaatctg tccaaagcgg
     1201 tcgcatccta cggcattatc ccggaagata taccgagccc gttcaacttg aatcagcata
     1261 tgatcatcga cggtaaaacc ggcaaaatgg aacatactca ggtgcggccg ccggaaggca
     1321 actatatgga tctgcgcgcc gaaatggatc tgctggtggc gctgagcgcc tgtccggatc
     1381 tcgccgtcgg cggtaaaccg gtcgatgtca tgatctacga ggaataggac gggagtgatg
     1441 gaataaatgg agtattggag tgttggtctt tccccatcac tccagtactc caacgctcca
     1501 ttactccttt tttcgttagc ccgagcgtga aggagaaccc cccatttcta tcgttatcgt
     1561 cgccatgccc tacggcgtct tggatccgca gaagctgtcg caggcttcgc gttcatgcgt
     1621 cgaacgcgcc ctcgactggt ggcaaaagtc gatgcaaacc accaagcaca agacctattt
     1681 ggtcttggcc ggctatgaca gcgaccaggg gcaggaaatc aaattgcgcc gggaactcgt
     1741 ccatagcagc gtgcaagatc cggaactcat cggcaatatc gtcgaggtta gcgccagcaa
     1801 cgaagttggc ctgacgcaga agatcagccg catccggacc ctgctgccgg tggagaccat
     1861 caccgtattc gtcgagtcgc gcaacgccat cagcgttaaa gcgatctttc agcgcaagtt
     1921 cggcaagacc ttgcaaatta gaaaattcaa agccaatttc gaattcaacc accagtggat
     1981 cacgacctcg acatcgatcg cctggtcggc acgcaactgg tttctccggc tctggttcga
     2041 attgaagaaa cggatggggc gcggattgcg caagaaaatt cgctactggt tcagagggta
     2101 aacggtaggg gcgaaaaatc tttcgcccct acagattatt taactacgcg acttttgaaa
     2161 tcttgccgtt cacctcggcc gagaaaccct tctccaacca ccagtgggcg acttcactcc
     2221 cggtcgcaaa ccacacgccc ttgtgcgact tcatgtggcc gatgatatcc gacaaagtct
     2281 tggcgcggcc ggcgcggctc gagatatagg ggtggaactg aaacggacaa aatgtcggcg
     2341 ccaggctcga ctcttcgtag aattggtcga acatcgcttt ccacaccacc aaagtatcgt
     2401 tggggttgcc cgagtcgcga tggccatagg tgcggccgtc accgaagggc tggcgcggca
     2461 gctcgacgag ctttttcttg ccgttctgcc agatgaacgg cagatcgtgg gagaacgaat
     2521 tgctattcca taagtaaccc tgctccatca agagctcgat gctgttgggg ctctgggtgc
     2581 aggaacgcca gcccaccggc cgcttctgcg cccggttgag gatcatgtcg gtggtccgct
     2641 tcatgacatc gcgctcctcg tccttgctca gatcgcgcgc cacctcgtga tgatagccgt
     2701 ggcctgcgat ttcatggccg gcggcgataa tcgccgtaac cgcgtccgga tagcgctcgg
     2761 cgatgccgcc gcaggtcaga aaagttgccc tcacttcatg cgccgcgagg atgcgtagga
     2821 tacgccagat accggtgttc ggaccatatt cagcctgggt gtattcttcg tgattaattt
     2881 ttccgttctt atcaggccgg acatcttcgc cgccctggaa atcgaaactg agcaaaaccg
     2941 cgatgcgggc atcgccgggc caatgaatct tgccgttgct gtgcatttct tctagtgaca
     3001 ttaatcctcc ttggttggga ccgcagatcg tgaactgccg attgcgcttt cgctgaccta
     3061 tagatattcc accggcttga cgcttgcaag taaaatttgc cgatccttgc tatgtcgctc
     3121 cgctggcgcc ggttgcgttg taggcgattg ggaaagattg tatagagagg gccggagatt
     3181 tcacggaggc attttatggc agagggaaaa ttcaatgtcg gcggcgtcgt gctcgagcgg
     3241 ccgttcaaga ttcgccggct cggtcacttc ggtttcaatc tggcgaacat ggaggcggga
     3301 cggcattttt atgtcgacct gttgggcttc aaggtctccg atgtcatgga ctattcgctg
     3361 cgcgccaaga gtcccgaaga cgtcgccggc ctgggcgatc ccaacggtta ttttacccgc
     3421 tacggcggcg atcatcacgc catggtgctt ttccccaagc gcgtgcgcga tgccctgggc
     3481 cgcagcgaag cgcctggggt gacgatcaac cagatcacct ggcaggtcgg cagcatgcgc
     3541 gaggtcggca tggcgattca gtggttcaac gaacatggca tcaagcagca gcgttccggc
     3601 cgcgacatgc ccggctccaa ttggcacacc tacctgctcg atcccgacgg tcacagcaac
     3661 gaactttact acggcatcga gcagatcggc tgggacggtt acagcaaacc gaagagcatg
     3721 tacgaccgcg gcttcaacac gccgcccgaa ctgccgcaga tctccgagtt cgacgaagtc
     3781 gaacaggcca aggccaatgg catcgacctg acctccggct atcaccatgt cgatgatctg
     3841 ccggcgagct tcgatgtcga cggcatcctg ttgccgcgcc ccttcaagat cgtccgcctg
     3901 ggtccggtgt acttattcgc cgatgatatg gccgcggctg aaaaatttta tcgccaacgc
     3961 atgggcttta cactgaccga ggaggtcaac tggcaaggcc atcgctgtct gttctttcgc
     4021 tgcaataccg aacatcactc actggcgctt tttcccactg cggcgcgcgc ggccctgggt
     4081 ctgagcggcc atagcaaaac cgccgccctc gggctgcagc tcgccaatta ccgccaactc
     4141 aaggacgccg tggcatttct ccgcagccag ggcgtgaaag tcagcgaagc gattcccagc
     4201 gaactccatc ccggcatcga tctcgccgcc cacgccaccg accccgatgg tcacgccatc
     4261 caactttatt gcgccatgga acagattggc tgggacggga aacctcgccc caaagaattg
     4321 cgccagccga aacccctgag cgactggccg gcaaccttgg aggcaaactc cgacgtctac
     4381 gccggcgagc cgttcctcgg cccgtggggg taatcaagta aggtgaattc aatccaatca
     4441 aagagggccg attcgatgct gcaatggacg atagccatca tcttcgtgat tgtgccggcg
     4501 cttgcacagc atgccgcggc gcaggaaaaa cggctcgatc cgctgatcgt ttcctacagc
     4561 tccgtc
//
LOCUS       DASQKS010000461         7197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5706859, whole genome shotgun sequence.
ACCESSION   DASQKS010000461 DASQKS010000000
VERSION     DASQKS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5706859"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..837)
                     /locus_tag="VE170_11925"
     CDS             complement(76..837)
                     /locus_tag="VE170_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ16191.1"
                     /translation="MAELVVQDIEKWFRREDGQPFKVIDRLSFRAKEGSVTVLIGPSG
                     CGKSTLLNSIVGLERLDRGDLTFVNNGKSQERPLIGYVFQSPRLLPWKTIGDNLRLAL
                     KGAGVAQGESEKRVLRYLELVGLEEYINQFPLYLSGGQRQRVGLARALAIESDLVLMD
                     EPFASVDELTARQLRQTTRRLCEQLHRTVLFVTHNLAEAAYMSDYVVTLTGRPARLDA
                     YVTNPLPTPRQWGPALYGFAAELETKVTLKVEEEK"
     gene            complement(838..1710)
                     /locus_tag="VE170_11930"
     CDS             complement(838..1710)
                     /locus_tag="VE170_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ16192.1"
                     /translation="MLSMAFDPNNQAAISDQAADIAGAATIPTFGNSAGRDLLGRILP
                     PLLAVSSVLGIWWLTYLIYPRLLPSPMSTLNEAHRLLSDGTFFFHMYQSLRRVFVGAT
                     VAMIFSVAVGIYMGTVRMGEHFFQPLVVLGLTIPGLMWALIAVMLFGINEFASYFAVA
                     VTTFPMLVINIWAGVKSLDKELMDMSHVFHFTKSMKITQVILPQLVSNIFAATRYGLG
                     LAWKVVVVVEMFGTSNGVGYQVMKSYQVFNMEGVIAWTLTFVVAMIVIEYGIINLVER
                     RLTAWRPKIDVWRR"
     gene            complement(1947..2438)
                     /locus_tag="VE170_11935"
     CDS             complement(1947..2438)
                     /locus_tag="VE170_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16193.1"
                     /translation="MATKLSVLLSLFLLLTGLGKVWAAADQLEEILDEANKARQEQKA
                     KELKAREQIEQLQRDQRASEMQQQLDQIKRQKTDPADGQKSQAEKTQQQLDQQKNDQQ
                     LQRLQSDQQLRRIEQDKDPLRQQQQINELQRQQQIRSLQDQIQRNQLQQDLDRTRQQQ
                     NNR"
     gene            complement(2444..3430)
                     /locus_tag="VE170_11940"
     CDS             complement(2444..3430)
                     /locus_tag="VE170_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16194.1"
                     /translation="MVLFAILLCAGVIDAKPTPAAEFSNKLRIGYPSSAVSTLPFDIA
                     KEKGFYSKAGLDVEYIQMRSALGPQAILNGNIQFFTSPQSAINAAVAGLPLVVVLSLY
                     RDTPWVLVTNKEINKAADLSGKRIAISDIRSSPYYFARAGLRKLGLDEKQVNLVTTGG
                     TSNSFATLATHQVAGAVLSPPFDEKAVSLGYKKFLFLGDLADIPYVGLFTSQAEIRAN
                     RERVRRTIGAVMNAIDWQKSNRSEAVKMIAARYQISSGEAERSYDTMVAILSPDGAIN
                     MKKVRGYLNLLREERPVADDLDPEKLVDFTMLPGVPNPAQSGLEMTASACQH"
     gene            complement(3515..4648)
                     /locus_tag="VE170_11945"
     CDS             complement(3515..4648)
                     /locus_tag="VE170_11945"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16195.1"
                     /translation="MSNHGCKVLDSDMHILEPADLWQRYLDAKFKSRAPVGLTDHVRD
                     LRLVGPDGIAWGRPADPAPGTLPSPGHIFHKNQKLFKPHNQRGWSSQVQLDAMAEEGI
                     DVAILYPSRGLNVLSVPDLDAEFAAALARAYNDWLYDFCQADPGKMIGAGMISPFDID
                     RAVQEAERCVKQLGFRAIFLRANIVNRHNWHEPYYEPLWAALEDLGVPLGFHEANNSL
                     APQSGDTFGYDFMLRHTYAHPVEQMLAVGAFCGGGILDRHPKLRVAFLEGNCGWLPFL
                     LWRLDEHWEQYGDQWAPGLQQPPSYYFKRQCYASVECDEEPVKYTIDYLGNERLVFST
                     DFPHVDTKYPRAVERFLELPIGDEDKRKILWDNCAIYYGMLGM"
     gene            complement(4788..5735)
                     /locus_tag="VE170_11950"
     CDS             complement(4788..5735)
                     /locus_tag="VE170_11950"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16196.1"
                     /translation="MPTVGFIGLGNMGGPVAGHIQRAGFPMIVCDLRAEATQPFCERG
                     ATVAESIIALVDRSEVVITALPMPDDVEKVAGEILQAIKPGTIYIDISTSPPSLLRNL
                     EPLFHAKGAHVLDAPVASGQPGAARGIHEVMVGGAREIFERAKPVLDAFGDQVIHAGP
                     LGSGSICKLVHQMINSTIAQAIAEGLTLGVKAGVDTVALWECVRRGMVGRMHVLHSQV
                     PQNVFRNNYETDTFPLKLLRKDVGLATALGRDLNVSLPLAHLAEQKLIEAVNRGWGDL
                     SAYTVTFKLQEEAAQVALRAEGVDPQKAARYISTNPKTD"
     gene            5879..6865
                     /locus_tag="VE170_11955"
     CDS             5879..6865
                     /locus_tag="VE170_11955"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ16197.1"
                     /translation="MGHMMNGKWVGDDRISADARGAFVRADSQFRNWITPDGSAGFSG
                     TGGFKAEPGRYHLFVSYNCPWAHRSIIFRKLKQLEDVVSMSLADKPKSAGWAYSEAID
                     DFHPEADGVFRLHQVYAAADAGYTGKVTVPTLWDRERRTIVNNESSEIIRMFNADFRN
                     HTNASYDYYPETLHGEIDKINELVYSHFNNGVYRAGFARSQEAYDEAVKRVFACLEQM
                     EEWLSQRRYLAGTRITEADWRAFPTLLRFDPVYHGHFKCNWRRVQDYPNLANYLRELY
                     QWPGIKETFDLEKTKAGYYGQRNVNPTGIVPIGPDMSHLEAAHDRGRLEAEG"
BASE COUNT         1498 a         1964 c         2148 g         1587 t
ORIGIN      
        1 ggccggccgg tgcttcggtt gctatggtga cgagcatcgt ccaagcggtc gcgattgaga
       61 cgaaaaattt acctgtcatt tttcttcctc cactttgaga gttactttgg tttcgagttc
      121 ggcggcgaag ccataaagcg ccggccccca ttggcgcggt gtcggtaatg gattggtcac
      181 ataggcatcc agacgcgccg gccggcccgt gagcgtgacg acgtagtcac tcatgtaggc
      241 ggcttcggcg agattgtggg tgacgaataa tacggtgcgg tgcagctgct cgcagagccg
      301 tcgagtggtt tgacgcaact ggcgcgcggt caattcatcg acgctggcaa acggctcgtc
      361 catgagtacg agatcggatt cgatggccag cgcgcgcgcc agaccgacgc gctgacgctg
      421 gccgccggaa agataaagcg gaaattgatt gatatattct tcaagcccga ccaattccaa
      481 atagcgaaga acccgcttct ccgattcacc ctgggcgaca ccggcgcctt ttaaagccag
      541 ccgcagattg tcgccgatgg ttttccacgg cagcaggcgc gggctttgaa atacgtagcc
      601 gattagcggc cgctcttggg acttgccatt gttgacaaag gttagatcgc cgcggtcgag
      661 ccgttccaag ccgacgatgg aattgagcag agtggacttg ccgcagccgg aaggcccgat
      721 caaaacggtg accgaaccct ctttagcgcg gaaggacaaa cggtcgatga ctttgaaggg
      781 ttggccgtcc tcgcggcgaa accacttttc aatatcttgg acgaccagtt ccgccatcta
      841 acgtctccag acgtcgatct tcgggcgcca ggcggtgagc cggcgctcaa ccagattgat
      901 aatgccatat tcgatgacga tcatggcgac gacgaaggtc agcgtccagg cgatgacgcc
      961 ttccatattg aagacctgat acgacttcat gacctgatag ccgacgccgt tgctggtgcc
     1021 gaacatttcg acgaccacga cgactttcca ggcgagcccc aaaccataac gggtggcggc
     1081 aaaaatattc gacaccaact gcggcaggat gacttgggtg attttcatcg acttggtgaa
     1141 atgaaacaca tggctcatgt ccatcagttc tttgtcgagg gatttcacgc cggcccagat
     1201 gttgattacc agcatgggaa aggtcgtcac cgctacggcg aaataggaag cgaattcatt
     1261 gatgccgaac aacatgacgg cgatgagcgc ccacatgagg ccggggatgg tcagccccaa
     1321 gacgaccagc ggctgaaaga aatgctcgcc catgcgcacc gtgcccatat agatgccgac
     1381 ggcgacgctg aaaatcatcg ccaccgtggc gccgacgaag acccggcgca aactttgata
     1441 catatggaag aaaaacgtgc cgtccgaaag tagccggtgc gcttcgttca aggtgctcat
     1501 cgggctcggc agcaggcgcg gatagattag ataggtcagc caccagattc ccaggacact
     1561 gctgacggcc aacagcggtg gcaggattct gccgagcaaa tcacgtcccg ctgagttgcc
     1621 gaaggtcggg atggttgcgg cgccggcgat gtcggccgct tggtcggaga tcgccgcttg
     1681 attgttggga tcgaaggcca tcgacagcat ttgtgctata gaccgcagac gctgtcaatc
     1741 gtcgcggtca atgcaacgcc gcggggcaac cagccgttct tgcttcggct agggtgcgac
     1801 aaatgtgtcg aggatcaaat attgccgata caattgtcat cacggcaggt ggttgaaatg
     1861 aagcaggctg gaatggcggc gtaaccaatt aagtcactgt aggtccgctg ctgaacaccc
     1921 acagttcacc ttgcaggcca cggctcttaa cggttgttct gctgctgtcg agtgcggtcc
     1981 aaatcctgct gcagctgatt gcgttggatt tgatcctgca atgagcggat ttgttgttgg
     2041 cgttgtagct cgttgatttg ttgctgctgg cgcagtgggt ctttgtcttg ttcgatgcgg
     2101 cggagttgct ggtcgctttg caggcgctgg agttgctggt cgttcttttg ttgatcgagc
     2161 tgttgctggg ttttttccgc ttgagacttt tggccatcgg caggatcggt cttttgccgt
     2221 ttgatttggt cgagttgctg ctgcatctcg ctggcgcgct ggtcgcgctg gagctgttcg
     2281 atctgctccc tggcttttaa ctctttggcc ttttgttcct ggcgcgcttt gtttgcttcg
     2341 tccaggattt cctcgagctg gtcggcggcc gcccaaactt tgccgagccc agtcaaaagg
     2401 agaaataaag aaaggagcac cgatagcttg gtcgccatat gcatcaatgc tggcaagctg
     2461 aagcggtcat ttcaagtcct gattgcgcag gattaggcac acccggcagc atggtaaaat
     2521 ccaccagctt ttccgggtcg aggtcatcgg cgaccggccg ctcctcgcgc agcagattta
     2581 agtagccgcg gactttcttc atgttgatcg cgccatcggg ggaaaggatc gcgaccatgg
     2641 tgtcgtagga gcgctcggct tcgccgctgc taatctggta gcgcgccgcg atcattttga
     2701 ccgcttcgct gcggttgctc ttttgccaat cgatggcgtt catgaccgcg ccgatggtgc
     2761 ggcggacgcg ctctcggttg gcgcggatct cagcttgcga tgtgaatagc ccgacatagg
     2821 gaatatcggc caagtcaccc agaaaaagaa acttcttgta gcccagggaa accgctttct
     2881 cgtcgaaggg cggactcaat accgcgccgg ccacctgatg ggtcgccaga gtcgcgaagc
     2941 tattcgaggt gccgccggtg gtgaccaggt taacttgttt ctcatccagg ccgagtttgc
     3001 gcagtccggc gcgggcgaag tagtagggcg acgagcggat gtccgagatc gcgatccgtt
     3061 tgccggacaa gtcagcggct ttgttgatct ccttgtttgt caccagcacc catggggtgt
     3121 cgcgatagag cgacagcacg acgaccagcg gcaatccggc cacggcggca ttgatcgccg
     3181 actgcggtga agtgaagaat tggatgttgc cgttgaggat cgcctgcggg ccaagcgcgc
     3241 tgcgcatctg gatatattca acgtcgagtc cggctttgct gtagaagcct ttctctttgg
     3301 cgatgtcgaa gggcaaggtc gagaccgccg aagaaggata accgatgcgc agcttattcg
     3361 agaactccgc tgcgggtgtc ggtttggcat cgatcacgcc ggcgcacagc aggatggcaa
     3421 agagcaccat gcgggtcgct ctagctcttg tttgcatgga accctgtggg gttgtccttt
     3481 cgtatgccaa acaccgaggc ctgtgcatcg acttctacat ccccaacatg ccgtaataga
     3541 tcgcgcagtt gtcccagagg attttgcgtt tgtcttcatc accgatgggc agttcgagaa
     3601 aacgctcgac ggcgcgggga tacttggtat cgacatgggg gaagtcggtg gagaagacca
     3661 gccgttcgtt gccgagatag tcgatggtgt atttcaccgg ctcctcgtcg cactcaaccg
     3721 acgcgtagca ttggcgtttg aaataatagc tcggcggctg ctgcaacccc ggcgcccact
     3781 gatcgccgta ctgctcccaa tgttcgtcca agcgccagag aaggaacggc agccagccgc
     3841 agttgccttc gagaaaagcg acccgtaatt ttggatggcg atctaaaatg ccgccgccgc
     3901 aaaacgcgcc gacggcgagc atttgctcca ccggatgagc ataggtgtgg cgcaacatga
     3961 agtcgtagcc gaaggtatcg ccggactggg gagcgagcga attgttcgct tcgtgaaaac
     4021 ccagaggtac gccgagatct tccaaggcgg cccacagcgg ttcatagtag ggctcatgcc
     4081 agttgtggcg gttgacgatg ttggcgcgga gaaatatcgc gcgaaagccc agttgtttta
     4141 cgcatcgctc ggcttcttgg acggcccggt cgatatcgaa cggggaaatc atcccggcgc
     4201 cgatcatttt accaggatcg gcttggcaaa aatcataaag ccaatcgtta taagcgcggg
     4261 ccagagccgc ggcgaattcc gcatccagat cgggaacgct gagcacattc aacccgcggc
     4321 tcggatagag aatcgccaca tcgatgcctt cctcggccat ggcatcgagt tgcacctgcg
     4381 acgaccagcc gcgctggttg tgcggcttaa acagtttctg atttttatga aaaatatggc
     4441 ccggcgacgg taaagttccg ggcgcgggat cagctggccg cccccaggct atgccgtcag
     4501 ggccgacgag gcgcagatcc cgcacgtgat cggttaggcc cacaggcgcg cggcttttga
     4561 acttcgcatc caggtagcgc tgccagagat cggcgggctc gaggatgtgc atgtcgctgt
     4621 ctaatacttt gcagccatgg ttggacataa taacctctcg aagttcaagc cgttcaaaac
     4681 attcaagtcg gtcaagccgt ggaataagaa agcagcagag gattgcacgg caaaacctcg
     4741 gaaatacgtt ttgaacgaat tgaacggctt gaactgtttg aacgcgttca atccgtcttt
     4801 ggattggttg aaatataacg tgcggctttt tgcgggtcca cgccttcggc gcgcagcgcc
     4861 acttgcgccg cttcctcctg aagcttgaac gttaccgtgt acgccgacag gtcgccccag
     4921 ccgcgattga ccgcttcgat tagcttttgc tcggcgaggt gcgccaacgg caacgacacg
     4981 ttgagatcgc gacccaatgc cgtcgccaaa ccgacatctt tgcgcagcag cttgagcgga
     5041 aaggtgtcgg tctcgtaatt gttgcgaaac acgttttgcg gcacctggct gtgcagcaca
     5101 tgcatgcgcc cgaccatgcc gcggcgcacg cactcccaaa gtgccaccgt gtcgacgccg
     5161 gctttgacgc cgagggtgag tccttcggcg atggcctgag cgatggtgct gttgatcatt
     5221 tgatggacga gtttgcatat gcttcccgag ccgagcggcc cggcatggat cacttgatcg
     5281 ccaaaggcgt cgagcaccgg cttggcgcgc tcgaagattt ctctggcgcc gccgaccatc
     5341 acttcatgga tgccgcgcgc cgcgccgggc tggccagacg ccaccggggc gtcgagaaca
     5401 tgggcgcctt tggcgtgaaa cagcggttcg aggttgcgca gcagtgatgg cgggctggtg
     5461 gagatatcga tgtagatcgt tcccggtttt atggcttgga gaatttctcc ggcaaccttc
     5521 tcgacgtcat cgggcattgg caaggccgta attacaactt cgctgcggtc aacgagcgcg
     5581 ataatggatt cggcgaccgt tgcgccgcgt tcgcaaaacg gttgcgtcgc ttccgcgcgc
     5641 aggtcgcaaa cgatcatcgg aaaaccggcg cgctgtatat ggcccgccac cggtccgccc
     5701 atgttgccca agcctatgaa tccgacggtt ggcatttctt acctcgttgg ttctgcttat
     5761 cgcagaagtg agattcgggt gtcaacgcgg cgtgccgtag tatcgagtcc taggtttcgg
     5821 cttggtcgtc ggggcgattt ttgttaagag tagacctccg gcgataggag aatgaattat
     5881 gggccatatg atgaacggca aatgggttgg cgatgaccgg attagcgccg atgcgcgcgg
     5941 ggcgttcgtg cgcgccgact cgcagtttcg taactggata acgcccgacg gcagcgccgg
     6001 tttttccgga actggcggat tcaaagccga gccgggacgt taccacctgt tcgtttcata
     6061 caactgcccg tgggcgcacc ggtcgatcat ctttcgcaag ttaaagcagt tggaagatgt
     6121 cgtctcgatg tcgctggccg acaagcccaa gagcgcgggt tgggcctatt cggaagccat
     6181 cgacgatttt catcccgaag ctgacggcgt atttcgcctg catcaagttt acgccgcggc
     6241 ggatgccggc tacaccggca aagtcaccgt gccgaccctg tgggatcgcg agcggcgcac
     6301 catcgtcaac aacgaatcgt cggaaatcat ccgcatgttc aacgccgact ttcgcaatca
     6361 caccaatgcc agctacgatt actacccgga aacgctgcac ggtgagatcg ataagatcaa
     6421 cgagctggtc tacagccatt ttaacaatgg agtgtatcgt gccggcttcg cgcgcagcca
     6481 ggaagcttac gatgaagcgg tgaagcgagt gtttgcctgc ttggaacaga tggaagagtg
     6541 gctcagccag cggcgctatt tagccggcac gcgcattacc gaagccgact ggcgcgcctt
     6601 tccgacgctg ctccgcttcg atccggtcta tcacggccac ttcaagtgca attggcgacg
     6661 ggtgcaagac tatccaaact tggcaaatta cctgcgcgag ctgtaccagt ggccggggat
     6721 caaagagaca tttgacttag agaaaaccaa ggcggggtac tacggccagc gcaacgtcaa
     6781 cccaaccggc atcgtgccga tcgggccgga catgagtcat cttgaggcgg cgcacgatcg
     6841 cggacggcta gaggcggagg gttaagagat tggccgcaaa gaacgcaaag ttcgcaaaac
     6901 cagaattacc agactcctat ctgcgtcaat ttgagtaatt tgtccgcttg cgatttcgcg
     6961 caaagcgaga aatcttatgt tgcgttctct tatgaaaatg ccgactaggg gcaaaaatcc
     7021 cccggtcctt cggcccgccc cctttggcaa agggggccat tgggggattt tccatcgttg
     7081 ctttgaacct ggcgcagctc attttcattt gctgtgttgt accgattggt acatgaatta
     7141 ctttgcgtgt ttgcggccct cttcctctgc tacgctatct tcggccctgt gtactcc
//
LOCUS       DASQKS010000462         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1511053, whole genome shotgun sequence.
ACCESSION   DASQKS010000462 DASQKS010000000
VERSION     DASQKS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511053"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="VE170_11960"
     CDS             complement(<1..1774)
                     /locus_tag="VE170_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ16198.1"
                     /translation="MNLFPATRTTPLPGGYMGKILRVNLTTGKLTDENLPEEPILKKF
                     IGGQALALYILMSELPIDATPFGAENKVVMMTGPLTGNGFTPGGTKMTAVYLSPLTNK
                     TLGRGATSGYWATYLKAAGYDGVIVEGVSENPVYLYVKDGKAEIRDGAKVWGLGTRDT
                     EDRLREIVGNRDARVLCIGPAGENLNRGAMLANDYNHFAAHSGGAVLGSKKLKAIVVA
                     GTLRPSHRDKAKLADAGLRWRNVLQQHDVKKKKTKYGHGDAWGALNNLNWRSTDIQPA
                     HIAGFADNDVVLRPCFQCARMCPWDAKLGEGEFKGTVVHFNAGGEWLDTFFNLGIKGN
                     AVLYLAEKINNLGIECSHFSCGAGVAFEAWEKGLLGPDRTGGLKLEWGNVEAVSKLLD
                     MAAHREGWLGNLLAEGPKQLAEALGGDAPNWAVHTKGGTPAMHEWRPLLGQMLRELVA
                     SGGMKPQGGGSKNPPPDLRENEHWGPLEMDSPEGWVWSHVLSEQYRQFTGIFGGCWFA
                     QESHKKDGLNSIVDAFNAITGWNFSMAEALQAGHRSMILQSLFGTQRGWIADFDWQDV
                     GPRFLEPIPDGKYKGFTIAKWLPGM"
     gene            complement(1857..2846)
                     /locus_tag="VE170_11965"
     CDS             complement(1857..2846)
                     /locus_tag="VE170_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16199.1"
                     /translation="MLQSLLVLFVLTVLASSTGAATLDDPARAEGEVVLYSSLNNEQI
                     VTLVDAFKKKYPAIKPSFYRGTSERVLQRANTEAKAGRFAVDVITSAGFQVQLMKESG
                     LTQRFVPPEAAAYDEGFKDPDGHWVNVHSLLNSMAYNTQLVKVGEAPKKYEDLLAPRW
                     KGRIGVNLQDPEWYVSLQRRWGKDKARNFLKALAAQQPAVRDGHNITAQLLAAGEYHV
                     VTNTYAHIAARIKGQGGPVQYVFDEPVITYVHPMVLAKGAPHPNAGKLLISFILSIEG
                     QRMLRDQGRIPAHRDVDPQVFSLRNVKLQASDPRQAKEYGPAGEEMRGIFGVR"
BASE COUNT          539 a          874 c          906 g          678 t
ORIGIN      
        1 tcatgccggg cagccacttg gcgatggtga aacctttgta tttaccgtcg gggatcggct
       61 ctaaaaatct cgggccgacg tcttgccagt cgaaatcggc gatccagccg cgctgggtgc
      121 cgaacaggct ttgcaggatc atgctgcggt ggccggcttg caacgcctca gccattgaga
      181 aattccagcc ggtgatggcg ttgaaggcgt cgacgatgga gttcaagccg tcttttttgt
      241 ggctctcctg ggcgaaccag cagccgccga agatgccggt gaattgccgg tattgttctg
      301 acagaacatg ggaccagacc cagccctccg ggctatccat ttccagcggt ccccaatgtt
      361 cgttctcacg cagatccggc ggtggatttt tcgagccgcc gccttgcggc ttcatgccgc
      421 cgctggcgac cagttcccgc aacatttgac cgagcagcgg gcgccactcg tgcatcgccg
      481 gcgtgccgcc tttggtgtgc acggcccagt tgggcgcgtc gccgccgagg gcttcggcta
      541 attgctttgg gccttcggcg agcaagttgc cgagccaccc ttcgcggtgc gctgccatgt
      601 cgagcaattt actaaccgcc tcgacgttgc cccattcgag tttcaaaccg ccggttcgat
      661 ccggaccgag cagccctttt tcccaagctt cgaacgccac gccggcgccg caggaaaagt
      721 ggctgcactc gatgccgaga ttattgatct tctcggcgag ataaagcacg gcgttgcctt
      781 tgatgccgag attgaaaaag gtatcgagcc actcgccgcc ggcgttgaag tgaaccaccg
      841 tgcctttgaa ttcgccctcg cccagcttgg cgtcccaggg acacatgcgg gcgcattgaa
      901 agcatggacg gagcacgacg tcgttatcgg cgaagccggc gatatgcgcc ggttgaatgt
      961 cggtgctgcg ccagttcaaa ttattcaggg caccccacgc gtcgccgtgg ccatacttgg
     1021 ttttcttttt cttgacatcg tgttgctgca agacgttgcg ccagcgcagg ccggcgtcgg
     1081 cgagcttggc tttgtcgcga tggctcggcc gtagcgtgcc ggcgacgacg atggccttca
     1141 actttttcga gccgagcacg gcgccgccgc tgtgggcggc gaaatgattg tagtcgttgg
     1201 cgagcatcgc gccgcggttc agattttcac ccgcggggcc gatgcacagc actctggcgt
     1261 cccggttgcc gacgatttcg cgcaggcggt cttcggtgtc gcgggtgccg agaccccaga
     1321 ctttggcgcc atcgcgtatc tccgccttgc cgtctttgac gtaaagatag accggatttt
     1381 ccgacacgcc ctcgacgatc acgccgtcgt aaccggcggc tttgagatag gtcgcccaat
     1441 agccgctggt ggcgccgcgg ccgagagtct tgttggttaa cggacttaaa taaaccgcgg
     1501 tcatcttcgt gccgcccggg gtaaagccat tgccggtgag cgggccggtc atcatgacta
     1561 ctttgttttc cgcgccgaag ggcgtcgcgt cgatcggcaa ctcgctcatt aagatataaa
     1621 gcgccaacgc ctggccgccg ataaattttt tcaagatcgg ctcttcgggc agattttcgt
     1681 ccgtcaattt gccggtggtc agattgacgc ggagaatctt gcccatatag ccgccgggca
     1741 ggggggtggt gcgcgttgcc gggaataggt tcatcgtgat tgcgacctcg cactcattga
     1801 ttcgttccaa tcgttccagt cgttcaaaac gttcaagccg ttcaaatcga tttggatcac
     1861 cgtacgccga agatcccacg catctcttcg ccggctggac catattcctt cgcctgacgc
     1921 ggatcgctgg cttgcagctt cacgttgcgc agggaaaaca cttggggatc gacgtcgcga
     1981 tgggcgggaa ttcgtccctg atcgcgcaac atgcgttggc cttcgatgga cagaataaag
     2041 ctgattagca gtttgccggc attaggatgg ggcgcgccct tggccaagac catcggatgg
     2101 acataggtaa tcaccggctc gtcgaaaaca tattgcaccg gcccgccttg gcctttgatg
     2161 cgcgcggcga tatgggcgta ggtgttggtg acgacgtgat attcgccggc agccagaagt
     2221 tgcgctgtga tgttgtgacc gtcgcgcacc gccggttgct gggcggcgag cgccttcaaa
     2281 aaattgcgcg ccttatcctt gccccagcgc cgctgcaggc tgacgtacca ctccggatct
     2341 tggagattca cgccgatgcg tcctttccaa cggggcgcca acaggtcttc gtatttcttt
     2401 ggcgcttcgc cgaccttcac cagctgcgta ttgtaggcca tggagttgag cagcgagtgg
     2461 acgttgaccc aatggccgtc gggatctttg aacccttcgt cataggccgc ggcttccggc
     2521 gggacgaaac gctgggttaa ccccgactct ttcatgagct gcacctgaaa ccccgccgaa
     2581 gttatgacgt cgacggcaaa gcggccggct ttcgcttcgg tgttggcacg ttgtaggacg
     2641 cgctcgctgg tgccgcggta gaaagacggt ttgatcgccg gatacttctt cttgaaagcg
     2701 tcgaccaggg tgacgatctg ctcgttgttt agcgagctat agagtacaac ctcgccttcg
     2761 gcgcgggcgg ggtcatccag ggtggcggcg ccggtcgagg aggctaggac ggttaagacg
     2821 aataaaacca atagcgattg cagcatcttt acactcgggt tctagacatg gccgacgaag
     2881 agatcgtcca tcggcagcat ggttttcatc aagccttgct cgatctggta gcggttcatc
     2941 gccgtcagcg tgcgtttctc gttctcaccc atgacgtagt catagggatc ttcgccg
//
LOCUS       DASQKS010000463         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4033516, whole genome shotgun sequence.
ACCESSION   DASQKS010000463 DASQKS010000000
VERSION     DASQKS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4033516"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VE170_11970"
     CDS             <1..357
                     /locus_tag="VE170_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ16200.1"
                     /translation="PKDPYLRARGKILIDYFLNYLHEPYWALRGEMIKKIEAERDQKL
                     IGETRKEVAARLQYLEKALGDKPYFLGDYSLTDIAMLPRFPRLEQYGVLPSAALPKLT
                     AWFERMKQRPAVQAIL"
     gene            386..1405
                     /locus_tag="VE170_11975"
     CDS             386..1405
                     /locus_tag="VE170_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16201.1"
                     /translation="MKIILLSVLLLAGITSQPLFADDFYKGKTVRVIVGGTAGGGFDV
                     YTRAMTRHMGKHIPGHPIFIVENMTGAATRIAAKYLHSVAKPDGLTFGIFNGYLILSQ
                     VLDPKGFDFNVRQFEWLGVPIQDNVACVLRKETGITNIEQWMAAKTPVKMGGLGPGNS
                     TSDAVRIVKATLNLPIQLVEGYKGTADLRLAADSGELGGACWAWESIRATWTKALQSG
                     EVNPILQVTPKKQPDLQHVPLALDLAKTEDAKQIIRAGGIAPSAVTRVYVTTPKTPKD
                     RIDILRKGFANTLKDPEFLLEAKKGGLEVNPLDGDEVKKVVDSLFSLNPQTVAKLASI
                     LTVKQ"
     gene            complement(1497..2528)
                     /locus_tag="VE170_11980"
     CDS             complement(1497..2528)
                     /locus_tag="VE170_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16202.1"
                     /translation="MRRTIFSAVTLAAVITGAHGAHGATASADFYQGKVMRIIVGFSA
                     GGGFDTFARTVARYMGKYVPGNPSIVVDNMPGAGSLIAANHIYRVAKPDGLTIGAFNG
                     NQILGQLVGAQGTNFDARKMQWMGAPGYNHDLCVLHQRTGIASAEQWIAAKTPLKLAG
                     SAPGSTTDDVAKVLKEAIGLPMRLVTGYKGTADMRIAVESGEMDGLCGFSWVSVKSTW
                     RRAVESGQAIIMLQSAPKAHADLPKVPLTISFAKTAEARQLIEAGVHQPGAITYGYSL
                     PPNTPQERVQLLRRAFIQAIKDPDLLNDANKANLEIAPASGEEIAGAIEKMFQTPPAV
                     IAKLKDTLK"
     gene            2670..3689
                     /locus_tag="VE170_11985"
     CDS             2670..3689
                     /locus_tag="VE170_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16203.1"
                     /translation="MQPRLALLTLLLALLTSADLPAQEFYRGKVIRVIVGGSAGGGFD
                     IYTRAMARHMGKHIPGNPSLVVENMTGAGTMIAAKYVHSSAKPDGLSIGMFNGGLILG
                     RVLGMKGIDFDMRELEYLGVPVQDNPVCALRKESGVTNMEQWAASKTPIKLGGLSPGN
                     STSDVARIIQSALNLPVQLVEGYKGTNEIRLAADAGELHGACWAWETLKSAWGKAIPA
                     GEVNVVLQVTAKKLPELPNAPLALDLAKSEEAKLLLRAGAIDPAAIVRVYVTSPRIAR
                     DRLQILRAAFAKTLTDPEFVAEAKKANLDINPLTGEEVKKLVDDLFKLNPTTKSKLIS
                     TLIPK"
BASE COUNT          836 a         1121 c         1042 g          719 t
ORIGIN      
        1 cccaaagacc cctacctgcg cgcccgcggc aaaatcttga ttgactactt tttaaactac
       61 ctgcacgagc cctactgggc cctgcgcggc gagatgatca aaaagatcga agcggaacgc
      121 gaccaaaagc tcatcggcga aactagaaaa gaagtagccg ccaggctgca atatctcgaa
      181 aaagccctcg gcgacaaacc atatttcttg ggcgattaca gtctcacgga catcgccatg
      241 ttgccgcgct ttccgcgcct ggagcaatac ggtgtcttgc caagcgcggc gctgccgaaa
      301 ctaacggctt ggttcgagcg catgaaacaa cggcccgcgg tacaagcaat cctgtagatt
      361 tctctgtttt aatcggagaa ttcacatgaa gataattttg ctcagcgttc tcttgttggc
      421 aggtataacg tcacagcccc tcttcgccga cgatttctat aaaggcaaaa cggtccgggt
      481 tatcgtcggc ggcacggccg gtggcgggtt cgacgtctat actcgggcga tgacccggca
      541 tatgggcaaa catattcctg gccatccgat tttcatcgtc gagaacatga ctggcgcggc
      601 gacgcggatc gctgcgaaat atttgcatag cgttgccaag ccggatgggc tgaccttcgg
      661 gattttcaat ggctacttga tcctgagcca ggtgctcgat cccaagggtt tcgatttcaa
      721 cgtccgccaa ttcgagtggc tcggcgtgcc gatccaagac aacgttgcct gcgtcctgcg
      781 caaggaaacc ggcatcacca acatagagca atggatggca gcaaagaccc cggtaaaaat
      841 gggcggactc ggtcccggca atagcaccag cgacgccgtg cgcatcgtca aagcgacctt
      901 gaatctgccg atccaattgg tcgagggtta caagggcacg gccgatcttc gcctggctgc
      961 cgacagcggc gagctcggcg gcgcgtgctg ggcttgggaa tctatccgcg cgacgtggac
     1021 caaagcgctg caatcgggag aggtcaatcc aatcctgcag gtgacgccga aaaaacagcc
     1081 cgacctacag catgtcccgc tggccctcga tttggcgaaa accgaagacg ccaagcagat
     1141 catccgtgcc ggcggcatcg ctccgagcgc ggtcacccgc gtctacgtga ccacgccgaa
     1201 gacgcccaaa gatcgaatcg acattttgcg caaaggcttc gccaacacgc tgaaagatcc
     1261 ggaatttctc ctcgaagcaa aaaaaggcgg gctagaagtc aatccgttgg acggcgacga
     1321 agtcaaaaaa gtcgtcgaca gcctcttttc actcaatccg cagactgtcg ccaagctcgc
     1381 cagcattctt acggtcaagc agtgacgatc tcgtaaatcg taaggggtaa ggggtgaagc
     1441 ggaggcgacc gatcaacgcc tcacccctta cccttcaccc ctcgcgattt atcgcatcac
     1501 ttcagcgtgt cctttagctt tgcgatcacc gccggtggcg tctggaacat tttctcgatg
     1561 gcgccagcga tctcttcacc cgatgccggg gcgatttcca gattggcttt gttcgcgtca
     1621 ttcaatagat cgggatcttt gatcgcttgg ataaatgccc tgcgcagcaa ttgaacgcgc
     1681 tcctgcggcg tgttcggcgg cagcgaatag ccgtaggtga tcgcgccggg ttgatgcacg
     1741 ccggcttcga tcaattggcg cgcctcggcc gtcttggcga aactgatcgt cagcggcacc
     1801 ttgggcagat cggcgtgagc cttgggtgcg ctttgcaaca tgatgatcgc ctgccccgac
     1861 tccaccgctc tgcgccaagt cgatttcacc gacacccacg agaagccaca caacccatcc
     1921 atttcacccg actccacggc gatgcgcatg tcagccgtac ccttgtaacc cgtcaccaag
     1981 cgcatcggta gcccgatcgc ttccttcaag actttcgcga catcgtcagt cgtcgatccc
     2041 ggcgcgctcc cggcgagctt aagcggcgtc ttcgcggcaa tccattgctc ggcgctggca
     2101 ataccggtgc gctggtgcaa tacgcaaaga tcgtgattat agcccggcgc gcccatccac
     2161 tgcatttttc gcgcatcgaa attcgttccc tgggcgccga ccaactggcc gagtatctga
     2221 ttgccattga aggcgccgat ggtgaggcca tctggtttgg cgacacggta aatgtgattg
     2281 gcagcgatca agctgccggc gcccggcata ttgtcgacga cgatcgacgg attacccgga
     2341 acatatttgc ccatgtagcg cgccaccgtg cgcgcgaagg tatcgaagcc gccgccggcc
     2401 gagaagccga cgatgatgcg catcaccttg ccctgataaa aatcagccga cgcggttgcg
     2461 ccatgagccc catgggcgcc ggtgatgaca gcggcgagag tcacagcgga aaaaatagtt
     2521 cgtctcatcg agattcctct ttccagccaa aaccaagatt ggcacataag ctacgcgaag
     2581 cggacgacag ccgtcaagcg gcgtgcgctt cggaaacatt gacaaggccg cgatttccaa
     2641 cataatcagc cgaactcgga ggtgtcacga tgcaaccgcg cttggcgcta ctcacgctat
     2701 tacttgcgct gctaacgtcc gcagacttgc cggctcaaga attctatcgc ggcaaagtga
     2761 tccgcgtcat cgtcggcggc agcgccggcg gcggcttcga tatctacaca cgcgccatgg
     2821 cgcgtcacat gggtaaacat ataccgggca acccgtcatt ggtcgtcgag aacatgaccg
     2881 gcgccggcac gatgatagcc gcgaagtatg tccacagcag cgccaagccc gacggtctgt
     2941 ccatcggcat gttcaacggc ggattgatcc tcggccgcgt cctcggcatg aaaggcatcg
     3001 acttcgacat gcgcgagctc gaatacttgg gcgtgccggt gcaggacaat ccggtttgcg
     3061 ccctgcgcaa ggaaagcggt gtcaccaata tggagcaatg ggcggcgtca aagacgccga
     3121 tcaagttggg cggcctgagt ccgggcaaca gtaccagcga cgtggcgcgc atcatacaaa
     3181 gcgctctcaa cctgccggta caattggtcg agggctacaa gggcaccaat gaaattcgtt
     3241 tggccgcgga tgccggtgaa ttgcatggcg cctgctgggc ctgggagacg ctgaagagcg
     3301 cctggggcaa ggcgatcccg gcgggcgaag tcaatgtcgt cttgcaggtg acggcaaaga
     3361 aactgccgga gctgcccaat gcgccgctcg ctctcgattt ggcgaaatcc gaggaagcca
     3421 agctgctgct gcgcgccggc gccatcgatc cggccgcgat cgtccgcgtc tacgtaacct
     3481 cgccgcgcat agccagagac cgtttgcaga ttctgcgcgc cgccttcgcc aagaccctga
     3541 ccgatccgga gtttgtcgcc gaagcgaaga aagccaacct cgatatcaac ccgctcaccg
     3601 gcgaagaggt caagaaactg gtcgacgacc tgttcaagct gaacccgaca accaagtcaa
     3661 aactgatcag cactttgatt ccgaaataat ccgcgaatgc tctgcattta ggagaccg
//
LOCUS       DASQKS010000464         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6144386, whole genome shotgun sequence.
ACCESSION   DASQKS010000464 DASQKS010000000
VERSION     DASQKS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6144386"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VE170_11990"
     CDS             complement(<1..839)
                     /locus_tag="VE170_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ16204.1"
                     /translation="MRIRFWGTRGSLAKPGPSTIRYGGNTSCVEVRADDGTLIILDCG
                     TGAHELGRSLIASGECPIRGHLLLTHTHWDHIQGLPFFAPLFVQGNEWDIYAPQGLGQ
                     RLEETLAGQMEYAYFPVTLGQLGATIRYHELSEGSFALGTVHVTAGYMNHPGLALGYR
                     LEAGGVLMVYATDHEPHSRHQAEGVDTMQIEPIHREDQRHVEFLAGADLVIHDAQYTL
                     AEYPNKRTWGHSPAELAVDFALAAGVKRLALFHHDPLRDDAALDRLVETCRRRAATGA
                     LEV"
     gene            complement(853..2211)
                     /locus_tag="VE170_11995"
     CDS             complement(853..2211)
                     /locus_tag="VE170_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ16205.1"
                     /translation="MTTLDQASIAELSARFSGVLLGPDQSGYDDARRIHNGMIDRRPA
                     LIARCLGTADIADAVNFARTRGLDLAVRGGGHNVAGRAVCDDGLMLDLSLMKGIHVDP
                     VRRTARAQGGVTWREFNRETQAHGLATTGGVVSTTGIAGLTLGGGLGWLMAKHGLALD
                     NLLSAEVVTASGATLRASKDENADLFWALQGGGGNFGAVSWLEYRLHPVGAVTSGLIA
                     HPFEHARDVLKFCREITRGLPDDLTIFGALLHAPDGAKIAAIIACHCGDPAAAEAALQ
                     PIKKFGTPIMDTIGPTTYEATNMMLDAGFPRGALNYWKSNFMMELSDQAIDTLIAQFA
                     ACPSPMSGLVLEHIHGAATRVGIDETAFPHRREGYNFLVAAEWLNPDDNGRNIAWARE
                     SYDLLRPCFAPGRYINYLADDDGEDAIAAAYGPNYPRLRMLKAKYDPTNLFHLNQNIR
                     PA"
     gene            complement(2374..2547)
                     /locus_tag="VE170_12000"
     CDS             complement(2374..2547)
                     /locus_tag="VE170_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16206.1"
                     /translation="MDLFAMRDSSFLPLQIALAREYKMAAFEWGTKAQVMFWPTEAPP
                     WNVRAATMRIAQV"
     gene            2621..>3519
                     /locus_tag="VE170_12005"
     CDS             2621..>3519
                     /locus_tag="VE170_12005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ16207.1"
                     /translation="MESQWSRRQFLKTTAAAVVLGTAEKAMTAGAGSLPKRRLGKTSA
                     MISCIGFGSGTRFCSIENEDAAQALLERAFTLGINYFDTAGSYTRRPIERLSEKRLGE
                     FIKPRRKEVFLATKIDPRDRDGALRSVETSLKFLQTDYIDLVQIHSLSDLDDLKRIAS
                     ANGVLGAIQQLKQQKVARLIGITGHNDGVAMADALRRYEFDTVLMALNAAQAANPIAQ
                     RKMEPIPAFESAALPVALEKNLGILSMKVMGQGMLVGSGAGRASAAELLQFNLSQPVA
                     SVVIGCEQLAPLEQNVQAAMNFTP"
BASE COUNT          710 a         1118 c         1035 g          656 t
ORIGIN      
        1 aagacctcga gagcgccggt ggctgcgcgc cggcggcaag tctctaccaa tcgatccagc
       61 gccgcgtcat cacgcagcgg atcatggtga aacagcgcca gccgcttcac gcccgccgcg
      121 agggcaaaat ccacggccag ctctgccggg ctgtggcccc aggtgcgttt gttcgggtac
      181 tccgccagcg tgtactgtgc gtcgtggatc accaggtcgg caccggctag aaattcaaca
      241 tggcgctggt cctcgcggtg gatcggttcg atctgcatcg tgtccacccc ctcggcttga
      301 tggcgcgagt gcggctcgtg atcggtggcg taaaccatta agacgccgcc ggcttccaaa
      361 cgatagccca gcgccagccc ggggtggttc atgtaaccgg cggtgacatg aaccgtgccc
      421 agcgcaaagc ttccttcgct aagctcgtgg tagcggatcg tcgcgccgag ctggcccagt
      481 gtcaccggga agtacgcata ctccatctgc ccggcgagag tctcctcgag acgctgcccc
      541 aaaccctgcg gcgcgtagat gtcccattcg ttgccctgga caaacagcgg cgcaaaaaag
      601 ggcaaaccct ggatatgatc ccagtgagtg tgggtgagca ggagatgacc gcgtatgggg
      661 cattcgcccg acgctatcag gctccggcct agctcgtggg caccggtgcc gcagtccagg
      721 ataatcagcg ttccgtcatc agcgcgcacc tctacacagg aggtattgcc gccgtagcgg
      781 atcgtgctcg gccccggctt tgccagcgag ccgcgcgtgc cccagaaccg aatgcgcatc
      841 gctattccta actcaggccg gccggatatt ctgattcaga tggaacagat tcgtcgggtc
      901 atacttggct ttcagcatcc gcaggcgcgg atagttcggt ccgtaggctg ccgcgatggc
      961 atcttcccca tcatcatcgg ccaaatagtt tatgtagcgc ccgggcgcga agcatggccg
     1021 caataagtca taggattccc gcgcccacgc aatattccgc ccgttatcat ccgggttgag
     1081 ccattcggca gcgaccagaa agttatagcc ctcgcgccgg tgcggaaacg ccgtctcgtc
     1141 gatgccgacc cgcgtcgctg cgccgtgaat atgctccaac actagtccgc tcatcggcga
     1201 cgggcacgcc gcgaactgcg caatgagtgt gtcgatcgcc tgatcgctca gttccatcat
     1261 aaagttggat ttccagtaat tgagcgcgcc gcgagggaag ccggcatcca gcatcatatt
     1321 cgttgcctcg taggttgtcg gaccgattgt gtccatgatc ggggtgccga attttttaat
     1381 cggttgcagc gcagcctcgg ccgcggcggg gtcaccgcag tggcatgcaa tgatagcggc
     1441 aattttggcg ccgtcgggcg cgtgcaggag cgcgccaaag atcgtcagat cgtccggcag
     1501 gcccctagtg atctcccggc aaaatttcaa gacgtcgcgc gcatgctcaa atggatgggc
     1561 gatgagaccg ctcgtcaccg cccccaccgg atgcaagcgg tactctaacc acgagaccgc
     1621 gccaaagttg ccgccgccgc cttgaagagc ccagaagaga tcggcgtttt catccttgct
     1681 cgcgcgcagg gtggcacccg aggcggtcac gacttccgcc gaaagcagat tgtccagcgc
     1741 taggccgtgc ttggccatga gccagccgag gccgccgccg agcgtcaaac ccgcgatgcc
     1801 agttgtggag accacgccgc cggtggtcgc cagtccatgg gcttgggtct cgcggttgaa
     1861 ttcgcgccaa gtaactccgc cctgggcgcg tgccgtgcgg cgcactggat cgacgtggat
     1921 gcctttcatg agcgaaagat ccagcatcag cccgtcatca cagacggctc ggccggcgac
     1981 gttatgaccg ccgccgcgca ccgccaagtc aaggccgcgc gtgcgcgcga agttgaccgc
     2041 gtcagcgata tccgccgtcc ccaggcaccg ggcaatcaat gccgggcgcc ggtcgatcat
     2101 accgttgtgt atccggcgtg catcatcgta gcccgattga tcgggaccca ggagcacgcc
     2161 cgaaaagcga gcggacaact cggcgatact tgcttgatcg agtgtagtca tgacagttcc
     2221 tccggcaccg cattaggcgg gtaggttttt aaatcgccga caatacgccg gcgggtccat
     2281 acgaagtgcg gcatgcaaaa agactctcgg cgataaccta tagtgatagt gctgcgtgac
     2341 cgcgataatc tttgcccaca ctcgccgcag gtttcacacc tgtgcgattc tcatggtggc
     2401 agcgcggaca ttccatggcg gcgcctcagt tggccaaaac attacctggg cttttgtccc
     2461 ccattcaaac gcagccattt tgtactcccg cgccagcgcg atttgcaagg gaagaaaact
     2521 tgaatcgcgc atggcgaata aatccatttg acgacaccac cacgggttgg agtatgatgg
     2581 cggcgctata gttcttcggt tggcaccgtt aggaggatcc atggaatcac aatggtcccg
     2641 tcgtcaattt ctcaaaacca ccgccgctgc cgtcgtgctt ggcaccgcgg aaaaggcgat
     2701 gaccgccggc gccggttcac tgccaaagcg acgcctcggc aaaacctcag caatgatctc
     2761 ttgcatcggt ttcggctccg gcacccgctt ttgttccatc gaaaacgaag acgcggcgca
     2821 agccctgctc gaacgggcgt tcacgctcgg catcaattac ttcgacaccg ccggctcata
     2881 cacgcgccgt ccgatcgaaa gattgagcga aaagcggctc ggcgagttca tcaagccgcg
     2941 gcgcaaggaa gtctttctcg ccaccaagat cgacccacga gaccgcgacg gcgcgctgcg
     3001 ctcggtggaa accagtctca agttcttgca aacggattac atcgatctgg ttcagatcca
     3061 cagcctgagc gatctcgacg atctcaagcg catcgcgagc gccaacggcg tgctcggcgc
     3121 catccagcaa ttgaaacagc aaaaagtcgc ccgcttgatc ggcatcaccg gccacaacga
     3181 cggcgtcgcc atggctgacg cgttgcgccg ctacgaattc gacacggtct tgatggcgtt
     3241 gaacgccgcc caagcggcaa atccgatcgc ccagcgcaag atggaaccga tccccgcctt
     3301 cgagagcgcc gctttgccgg tggcgttgga gaaaaaccta ggcatcctgt cgatgaaagt
     3361 gatgggccag ggcatgctcg ttggcagcgg cgccggccgc gcctccgccg ccgagctttt
     3421 gcaattcaac ctgagccaac ccgtcgccag cgtcgtcatc ggctgcgagc aactggcgcc
     3481 gctcgaacaa aacgtgcaag ccgcgatgaa tttcacgcc
//
LOCUS       DASQKS010000465         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4034426, whole genome shotgun sequence.
ACCESSION   DASQKS010000465 DASQKS010000000
VERSION     DASQKS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4034426"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..469
                     /locus_tag="VE170_12010"
     CDS             71..469
                     /locus_tag="VE170_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16208.1"
                     /translation="MAFNSQALLANLTEREQVLGHHSAEGQAIRLLSRALSGWTSGNL
                     AATDVVDMSGQAVEDWLKRRLQVSPWSAKGLTTLLAAAVAAGLLAPSEAERLELLAGF
                     RAGSNARVFTPTEIETVLQTSIEIVERHWT"
     gene            466..1890
                     /locus_tag="VE170_12015"
     CDS             466..1890
                     /locus_tag="VE170_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16209.1"
                     /translation="MTQSQINWLLLSNAALGNFLAGTSSRIFSVSLPTVANSLETTIV
                     GISWAVISYQISQISLSLIFGRIGDIYGRHTLFGLGLLVSSLASLACGLSQNVTQLII
                     FRMFQGVGASMTQSQGRALALESMPKESSGKAQGFMTTAFHTGVLIGPSIGGLIIDYI
                     HWRAIFFFLIPIAMCAMALTLVNRAKGNVPPRSALADRSVDYLGALLLIGATIALIAA
                     VDHRIMESLAPELRVALVVGFFVVFLGFLYREKTTPSPILDLSLFRIRMFTLSSLALL
                     LVGVAQVMIGFVLPFYLQDVLNLKPSFIGLLFVSAPIFTVTLSPLVGWAVDKVGPRLP
                     ATVGIAFLAGAGFVGSYLRTDSHWLSAVLVLGLWGLATALFFTSNHTAMITCVPTQHR
                     GVATGAIYVMFGLGTTFGVSLGTMLLTASFRYFTGDPSATPTAANPGMFVRSMNFSFM
                     IGGSMALLAMLLSALRGDRRVSTH"
     gene            1940..2734
                     /locus_tag="VE170_12020"
     CDS             1940..2734
                     /locus_tag="VE170_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYJ16210.1"
                     /translation="MTTSRWLNKLLAVLMVLSAGCSREESRVPAKSSEPPAGAAASPS
                     ASATSTPNNSTPGHGVGTITGKVVFKGTHGPGKVTIGKDKEVCGEFKLDPVLVVGSQG
                     EVKNAVIQIADLKQTVSAPKEAVIDQMKCEYVPHVSAIPAGATVKIKNSDGILHNVHS
                     FSEKNSPFNRAQPKYLKEISEKFIHPEVISLRCDVHGWMSGWIVVTESPFYYVTADDG
                     SFKLESVPVGKYTLEVWHETLGKTTQSVEVKLGEVTHVTFEFQMKK"
     gene            3000..>3588
                     /locus_tag="VE170_12025"
     CDS             3000..>3588
                     /locus_tag="VE170_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16211.1"
                     /translation="MNEAITSSSAALKRYGLVALAFLHIAVGRGLHGTFGVFFVAMLD
                     AFGWSRATTAGAISLAIIFEGACLPWAGGLIDRIGGRKTLIFGGLVLAIGLSFASTIA
                     SIWHLYFWIGIVAAMGIALIGMVPHVAIITRAFPERKGTALGIAWAGGGVGIVLLVPA
                     TQLMIDRWGWSTAYIGLAVVTALLVIPPVLVFLPRN"
BASE COUNT          683 a          976 c         1044 g          885 t
ORIGIN      
        1 acaaggattc taccttgtcc ttggtgatct tcgcggtgaa ttatgtcaaa acagaggacc
       61 tgtatttgcc atggcattca attcacaggc gttgctcgcc aatctcaccg aacgggaaca
      121 agttctcggc catcattcag ctgaagggca ggcgatccgc ttgctcagcc gtgcgctcag
      181 cggttggacg tcggggaatt tggcggccac cgatgtcgtg gacatgtctg ggcaagcggt
      241 ggaggattgg ctgaaacggc gcttgcaagt gtctccctgg tcggcaaaag gcttgacgac
      301 attgctcgcc gcagcggtcg cggccggttt gttggcgcca agcgaagccg agcgcttgga
      361 gctattagcc ggttttcgag ccgggtccaa tgcccgcgtg tttaccccca ctgagataga
      421 aaccgttctg caaacctcga tcgagatcgt cgagcgccac tggacatgac ccaatcacaa
      481 atcaattggc ttctgctgag caatgcggcg ttgggaaact ttcttgccgg cacttcgagc
      541 cggatttttt cggtatcgct gccgacggtg gcgaatagct tggaaaccac catcgtcggc
      601 atttcttggg cggtgatctc ctatcaaatt tcccagatca gcctgtcgct aatctttggc
      661 cgaatcggcg atatctacgg ccgccacaca ttattcggtt tgggtttgct ggtctcgtcg
      721 ctggcatcgc tggcctgcgg tttgtcgcag aacgtgacgc agttaatcat ctttcgcatg
      781 tttcagggtg tcggcgcgtc gatgacgcaa tcccaaggcc gggctttggc gctcgaatcg
      841 atgccaaaag aatcgtccgg caaggcgcag ggcttcatga ccacggcgtt tcacaccggg
      901 gtcctgattg gcccgagcat cggcggtttg atcatcgact atattcattg gcgcgcgata
      961 tttttctttc tgatcccgat tgccatgtgc gcgatggcat tgaccttagt gaatcgtgcc
     1021 aaaggcaacg tgccgccacg gtcggcgctg gccgatcgca gcgtcgatta tttgggggcg
     1081 ttgttgttga tcggtgcgac catcgctttg atcgccgccg tcgatcaccg catcatggag
     1141 tcacttgcgc cggagctgcg cgtggcgcta gtggttggct tcttcgtcgt ctttctcggt
     1201 tttctctacc gggaaaaaac cacgccgagc ccgatcctcg atctatcgct atttcgcatt
     1261 cgcatgttca ccttgagtag tctggcgctt ttgctggtgg gcgtcgcgca agtgatgatt
     1321 ggcttcgttt tgccgtttta cctgcaagac gtgctcaact tgaaaccttc tttcatcggc
     1381 ttgctgttcg tcagcgcgcc gatctttacc gtgacgcttt cgcctttggt cggatgggcg
     1441 gtggacaagg ttgggccgcg cctgccggcg acggtgggca ttgcgtttct cgccggtgcc
     1501 ggcttcgtcg gctcttacct gcgcaccgac tcccattggt tgtcggcggt actggtgctg
     1561 ggcctatggg ggctggcaac cgcgctattt tttacctcga accatacggc gatgattacc
     1621 tgcgtgccga cacagcatcg cggcgtcgct accggggcga tctatgtgat gttcggcttg
     1681 ggcacaacgt ttggcgtctc cctcggcacg atgctcttaa ccgcttcgtt tcgctacttc
     1741 accggcgatc catcggcgac gccgaccgcg gccaatcccg gcatgttcgt gcgctcgatg
     1801 aacttcagct tcatgatcgg cggatccatg gcattgctag cgatgctgtt gtccgcgctg
     1861 cgcggcgatc gccgtgtttc cacccattga gcttgtgcga acatgagagt cttgtagtct
     1921 acagccggag gaacttgcga tgacgacatc tcgttggctc aacaagctgc ttgccgttct
     1981 catggtattg tcggctggct gctcaaggga agaaagccgc gtaccggcga agtcttccga
     2041 gccgccggct ggggcagcag catctccaag cgcatcggca acgtcgacgc caaacaattc
     2101 gactcccgga cacggcgtcg gcacgatcac cggcaaagtt gtgttcaaag gcacccatgg
     2161 gccgggaaaa gtgaccatcg gcaaggataa agaagtctgt ggcgagttta agctcgatcc
     2221 cgtgctcgtg gttgggagcc aaggagaagt caaaaacgcg gtgatccaaa tcgctgactt
     2281 gaagcagacg gttagcgcac ctaaagaagc ggtgatcgac cagatgaagt gtgaatacgt
     2341 gcctcatgtg tcggcaattc ccgccggcgc tacggtcaag atcaaaaata gtgacggtat
     2401 cttacataac gtccactcct tcagcgagaa gaattctcca ttcaaccggg cacagcccaa
     2461 gtatctgaag gaaatcagcg aaaagttcat ccatccggaa gtcatttcgt tgcgctgcga
     2521 cgttcatggt tggatgagcg gttggatcgt tgtcaccgag agtccttttt attacgtgac
     2581 cgccgacgac ggcagcttca agctggaaag tgtcccggtg gggaaatata cgctggaggt
     2641 ttggcatgag acccttggca aaaccaccca atccgttgaa gtgaagttgg gcgaggtgac
     2701 tcacgtgacg ttcgagttcc agatgaagaa gtagccgcga actcctttca gtgaataggc
     2761 aattctccgc acaaagggaa aaaattccac atggtcgagc gtttttccct cgtgcgagtc
     2821 acgtgtccaa gtgtccacca agtgtccaag ttgagataag ttgatagaag ttgacgtgat
     2881 gaagtggagt tgaaaatctt ttattttcaa cctgatacga tttaaggcaa cttcaaagaa
     2941 cctgcctttt agaattagac attcagccgc gcccgagtta ttgctaacgt ttcatcgtca
     3001 tgaatgaagc tattacctct tcgtctgcgg cgctcaagcg ttacggtctc gtcgcgctgg
     3061 cgtttttgca tatcgcggtc ggccgcggat tgcacggcac gttcggagtg ttctttgtcg
     3121 ccatgctgga tgccttcggt tggtcgcgcg cgacgaccgc cggcgcgatc tcgctggcga
     3181 tcatattcga aggtgcttgt ctgccctggg ccggcggatt aatcgaccgt atcggcgggc
     3241 gcaagacctt gatcttcggc ggtcttgtgc tcgcaatcgg attaagcttc gcgtcgacca
     3301 tcgcctcgat ctggcacttg tacttttgga tcggcattgt cgccgccatg ggcatcgcgc
     3361 tgattggcat ggtgccccat gtggcgatca tcacgcgcgc gtttcccgaa cgcaaaggaa
     3421 ccgccctcgg tatcgcctgg gccggcggcg gggttggcat tgtgctgctc gtgccggcca
     3481 cccagttgat gatcgatcgc tggggctggt caacggcgta catcggtctc gcggtagtga
     3541 ccgcattgct ggtgattccg ccggtgctgg tttttctgcc gcgcaatg
//
LOCUS       DASQKS010000466         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5708383, whole genome shotgun sequence.
ACCESSION   DASQKS010000466 DASQKS010000000
VERSION     DASQKS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5708383"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VE170_12030"
     CDS             complement(<1..434)
                     /locus_tag="VE170_12030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ16212.1"
                     /translation="MSSSWENVKVDGSNMRAYVSSPEGTTKVPAIVIVQGQTGVDDFV
                     QFTRMVASEGFVGAAPDLYHRDSADCKDDAPTRRMRLTDETVSADVNATVEYLKSHPS
                     VDPARVGIVGFCMGGRAVYLMSALNPDLKAGVMYYGGDIFHA"
     gene            complement(498..1529)
                     /locus_tag="VE170_12035"
     CDS             complement(498..1529)
                     /locus_tag="VE170_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16213.1"
                     /translation="MRKEILLSLLMLTASVATAAAQTPFYQGKTITIITGSQTGDVFD
                     IYARMLAAHMGKNIPGNPNLVVQNMTGAGHIVAANFVYSVAKPDGLTLLAPNPNLYVD
                     QLVGRPEIKFDWAKFAWIGNASRTRDMLYMRTDAPFKTMEDVRNAKEPPKCGATGTGT
                     TGFMVPRLLEETIGAKFNVITGYKGGNEVDLAVERGEIQCRAFSVTAFFAREPFHTWR
                     KNNFARAIIQMGEKRDSRLADVPTLNELMEKYKTPDIGRRVALVVTASEIFQRPYMGP
                     PGMRPELVKILREAFQKTLKDNAFLEEVKSKKLDAEYTSGEEMETMAKEMMSQRPEVV
                     ERLRKVLGN"
     gene            complement(1803..>2317)
                     /locus_tag="VE170_12040"
     CDS             complement(1803..>2317)
                     /locus_tag="VE170_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16214.1"
                     /translation="GDIITHCYTPQKPSIIDDQGKLLPLVRRAKERGVIFDVGHAGGH
                     FDFALVERAMGEGIIPDIISSDLHGRLSQPGFGVVGDLPTVLTKFLPLGLSLDEIIAK
                     CTVDAARAVGWQDRIGSLEVGREADIAVLQVVDEPVMLRDSVGGEKLHNQRIAAKWTM
                     RAGEVFQGRG"
BASE COUNT          459 a          686 c          646 g          526 t
ORIGIN      
        1 caagcatgaa aaatatcgcc gccatagtac atgacgccgg cttttagatc cggattgagc
       61 gccgacatga ggtagacggc gcggccgccc atgcagaagc cgacgatgcc gacgcgcgcc
      121 gggtcgactg aaggatgcga cttgagatat tcgacggtgg catttacgtc cgcgctcacg
      181 gtctcatccg ttaagcgcat gcggcgcgtc ggtgcatcgt ctttacagtc cgccgaatcg
      241 cgatgataaa gatccggcgc cgcgccgacg aatccttcgc tcgcgaccat gcgggtaaat
      301 tggacaaaat cgtcgacgcc ggtctgaccc tggacgatga cgatagcggg aactttggtc
      361 gtgccttccg gcgagctgac ataggcgcgc atgttcgaac cgtcgacttt gacgttttcc
      421 cacgatgaac tcatgataaa cctccgaagt gccggggcac ggttattgac cccgaatgga
      481 ttgccttatt tgcaaaacta gttccccaat actttcctca atcgttcgac aacctcgggc
      541 cgctgcgaca tcatctcttt ggccatggtt tccatctctt cgccggaggt gtactcggca
      601 tcgagcttct tgctttttac ttcttccaaa aacgcgttgt ccttcagcgt cttctgaaac
      661 gcttcgcgga gaattttcac cagctcgggg cgcatgcccg gcgggcccat gtaggggcgt
      721 tgaaagattt ccgacgcggt taccaccaaa gccacgcggc ggccgatgtc gggagtctta
      781 tatttttcca tcaattcatt gagcgtcggc acgtcagcta accggctatc tcgcttttcg
      841 cccatttgga taatcgcccg cgcgaagttg tttttgcgcc aggtgtgaaa aggctcgcga
      901 gcgaagaatg ccgtgacact gaaggcccgg cactggatct cgcctcgttc caccgccaag
      961 tcgacttcgt tgccgccttt gtaaccggtg atcacattga atttcgcgcc gatggtttcc
     1021 tccaacagcc gcggcaccat gaatccggtg gttccggtgc cggtggcgcc gcacttcggc
     1081 ggctctttgg cgttgcgcac atcttccatg gttttgaaag gagcgtcggt gcgcatgtag
     1141 agcatgtccc tcgtgcgcga agcattgccg atccaggcaa actttgccca gtcgaacttg
     1201 atttcgggcc ggcccaccag ttgatcgacg tagagattgg ggttgggcgc cagcaaggtc
     1261 agaccgtcgg gtttggcgac gctgtaaacg aaattggccg cgacaatatg gccggcaccg
     1321 gtcatattct gcacgaccag attgggattt ccgggaatat tttttcccat atgcgccgcg
     1381 agcatgcgcg cgtagatatc gaaaacgtcg ccggtctggc tgccggtgat gatcgtgatc
     1441 gtcttgcctt gataaaaggg cgtttgcgcc gccgccgtcg caaccgatgc ggtcagcatc
     1501 aataatgaaa gcagaatttc ttttcgcatt gttccctctc tttgcgatcc atcctttgtg
     1561 tctcctttga aatacacgta aggacagggt cttcacaatg acgatgtcac ctggatcccg
     1621 tcatactcgc gaaagcgggt atccaggcta attgggcgca gaccaaacct ggattcccgc
     1681 gtgcgcggga atgacgcgtc aagcaaaccg cccatggagc gaatcccact caatttcata
     1741 ttctatagac gagcgtaagc tcatggacgc tttgtggtga atctccgcat tccggtacca
     1801 tctcatcccc gcccttgaaa tacttcgccg gcgcgcatcg tccacttggc ggcgatgcgt
     1861 tggttgtgca acttctcgcc accgacggag tcgcgcagca tcacgggctc gtcgacgact
     1921 tgcagcaccg cgatgtccgc ttcgcggccg acttcgaggc tgccgatccg atcttgccag
     1981 ccaaccgcgc gcgcggcgtc gaccgtgcac ttggcgatga tctcatcgag actcaagcct
     2041 agcggcaaga attttgtcag caccgtcggc aagtcaccga cgacgccaaa gccgggttgg
     2101 ctcaatcgcc cgtgcaggtc cgagctgatg atatcgggta tgatcccctc gcccatggcg
     2161 cgctcgacca gagcgaaatc gaaatggccg cccgcgtgac cgacatcgaa gatcacgcca
     2221 cgctccttgg ccctgcgcac gagcggcagc agcttgcctt ggtcatcgat gatcgacggt
     2281 ttctgcggcg tgtagcagtg ggtgatgatg tcgcccg
//
LOCUS       DASQKS010000467         9224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3194985, whole genome shotgun sequence.
ACCESSION   DASQKS010000467 DASQKS010000000
VERSION     DASQKS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3194985"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VE170_12045"
     CDS             complement(<1..1301)
                     /locus_tag="VE170_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HYJ16215.1"
                     /translation="MSIVGHYYDGGLTPAHAASSAATPAQNLPDFINLAKKVGPSVVN
                     VSTSRIRGAAQEAPSPFGDNDPGEEFWQRFFGGRIPRGPQRQSGIGSGFIVDGNGTIL
                     TNYHVVDGAQKLSVTLSDGKNYDAKVVGKDQKSDMAVIKIEAGRDLPAVALGDSDRLE
                     VGEWVMAIGNPFGLDHTVTSGIVSAKGRNIGQGPYDNFIQTDASINPGNSGGPLINLR
                     GEVVGINTAIFSQSGGNIGIGFAIPSNAVKELLPQLRDKGKVVRGFIGVSVQKVSPEI
                     AESLSINPARGALVADMVNGGPADHAGVKIGDVIVEFDRRPIKDSADLPQQVARVVPG
                     AAVQIKVIRNGKETPLSLTIGEMKETEVAAAAQEGELGLTVQPLTPQLAETLGVERGA
                     GLVISAVKPGSAAEEAGLRSGDLLVEINRRPVKNLADYNRE"
     gene            complement(1482..1940)
                     /locus_tag="VE170_12050"
     CDS             complement(1482..1940)
                     /locus_tag="VE170_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16216.1"
                     /translation="MGAKREKVSTSKANHERQPFWVKDNNNGPRMQLDERAMAIFQPD
                     VLIESQYLATQTRRVHLDAERVLMFAVLRDAVSCFQDNVGSSCKRKQAMHHDAEEWIL
                     DNDRSHLFAFDNVCEILGLDPQYLRAGLLRWKSAALQGRPVKARAQSLAG"
     gene            2158..3336
                     /gene="ribB"
                     /locus_tag="VE170_12055"
     CDS             2158..3336
                     /gene="ribB"
                     /locus_tag="VE170_12055"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HYJ16217.1"
                     /translation="MPLATIEEAIDEIRQGRMVILTDDKDRENEGDLCMAAEKVTPEA
                     INFMATHGRGLICLTLTDERVRHLGLAMMVSDNTSPFGTAFTVSIDSAQGITTGISAA
                     DRAKTILDSIADDAKPQDLATPGHIFPLRARNGGVLVRAGQTEGSVDLARLAGLKPAG
                     VICEIMKDDGTMARQPDLMRFAKKHKLKVVTNADLIQHRLNYDSLVYRAASAPLPTRI
                     GGEFQAIVYNTHVDESEHLALIKGQISPNEETLVRVHTKYIPGDVFGFELLNTGAVIQ
                     RSMELIAKAGKGVILYLQPKIKAHHPAMVAYPKVEGKQQKGMNASFVYQADFKEYGIG
                     AQILRDLGVRKMRLLTNNKKHLVGLRGYGLEVTSLVPIPRENPLRATTKHPRAKAKHA
                     "
     gene            3382..3591
                     /locus_tag="VE170_12060"
     CDS             3382..3591
                     /locus_tag="VE170_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYJ16218.1"
                     /translation="MPIFEYTCRDCDQQFETLVLSIRDKISCPKCHSQQVNKQLSVFA
                     ASAAGADSEPSGGACTGTPQSCGCR"
     gene            complement(3721..4251)
                     /locus_tag="VE170_12065"
     CDS             complement(3721..4251)
                     /locus_tag="VE170_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16219.1"
                     /translation="MADEKTPAFEETKWWIDRRAVKERDKSAAPPVRGIIEKWAVDLL
                     QNYNQDEAAKNLVDINLKIFKELKVEPGQWGYYSLDIGKYPPDKLEKIKAALPLPPRA
                     RGIVIDAGYSVRVMITGEPVGERYVDINFLDTPSLDYWVKGQLVEERVFGNVGEALKR
                     IRLDLLSYMTPSEDSA"
     gene            complement(4288..5280)
                     /locus_tag="VE170_12070"
     CDS             complement(4288..5280)
                     /locus_tag="VE170_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HYJ16220.1"
                     /translation="MGKIRLTLSCWDYDRTRALQDGRVEVEGVDLTYLPLRVEETFWR
                     MLRYGEFDVAEMSTGSFLMSRDKGSPRLIGIPVFPSKTFRHSCIYINTDSGIKKPADM
                     TGKRVGVPEYQITMATWARGILQHEYGVLPEKMHWFTGGEETPGRQDKVQHKLPPNID
                     IQPIAGDKTLSGMLERGEIDAMISAHMPSPFVRRHPKVQRLIPNFREVETDYFKRTKI
                     FPIMHTIVIREEFYEQHPWIAQSLFKAFNESKRICQEAMYEFSALKYMMAWSIDEMEK
                     EREALGPDPWAYGLEANRHILETLVQYTHEQGLISKKLDVDSLFAKSTLEDFKI"
     gene            complement(5312..5512)
                     /locus_tag="VE170_12075"
     CDS             complement(5312..5512)
                     /locus_tag="VE170_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16221.1"
                     /translation="MEKIDPQPDHLDLERYHPGAQVNYKGKLYLIQRRTTLASGEAAV
                     VLQSDREQFVVSAHQFLANVGS"
     gene            5864..6778
                     /locus_tag="VE170_12080"
     CDS             5864..6778
                     /locus_tag="VE170_12080"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYJ16222.1"
                     /translation="MGTGAVGGYFGAKLASAGHQLAFIARGRHLQALRKNGLWVTSQN
                     GNLRIDNAQFTDDTATIGKVDAILFCVKSYDTEVAAEQIKQLVSDQTLILSLQNGVDN
                     PDKLARVFDPGQILPAVVYVGAQLTAPGLVVHANGGRIIFGQIDAGVSESSNSLRQIL
                     SNAGIPCEISADIQKIQWTKLLWNAPFCAIGCLAHANVKQIVESESLTALALDCMTEV
                     QGAAHARNIDLRREQFDEIMTFSRTLGQFKPSMLQDLEAGKPLEYEAFNGIVVQTLRQ
                     AGAPAPINQVFYATLKFLDQRIRAQLKA"
     gene            6788..7684
                     /locus_tag="VE170_12085"
     CDS             6788..7684
                     /locus_tag="VE170_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ16223.1"
                     /translation="MPIIQSKKVPLLLWLFLLCATPLAYAQGTQKELVARGQYIFAVS
                     GGCACHTEVKGVHNAGARPFRIPLGTVYSTNLTSDNETGLGSWTDQQIIDAIIKGLRK
                     DGSRQLPLMPYTLYSGMAQEDLKALVAYLRTLKPVKKATPELTTSTPFMRSVVTEIWL
                     KAFGIFYESPAQAPKSGIQRGKYLANHVAICGDCHTPRNSFGVPNQSLYMAGSPKDGL
                     IGELVPNITPDKETGIGDWKREEIAELLLTGVKPDSDTVQGLMEEVIRGTTHGYKDMT
                     KEDVLAIADYLKSIPAIKNKMK"
     gene            complement(7908..8891)
                     /locus_tag="VE170_12090"
     CDS             complement(7908..8891)
                     /locus_tag="VE170_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ16224.1"
                     /translation="MELEQFQQIFKGLETEVQKVIVGNGNVIRKVLIAFFSGGHVLLE
                     GVPGLGKTLLVKSLSHALGMSFKRIQFTPDLMPSDIVGTEVLTEANGRREFQFKKGPI
                     FAHVVLADEINRATPKTQSAVLEAMEEKQVTVFGESHILESPFIVLATQNPIELEGTY
                     PLPEAQLDRFFFKLLVAPPAPDELREILKRTTGVEIGDAAKVLPEDGGKLVNEMKQLL
                     RQVLIAPPIEDYVVRLVHATQGNGQSGGTAGSIKQYIRFGSSPRGAQAIILGAKGNAL
                     ADGRVHVSYEDVENVIYPALRHRIILNFQAEAENVSADQVIAEVMKQVRRN"
     gene            complement(8906..>9224)
                     /locus_tag="VE170_12095"
     CDS             complement(8906..>9224)
                     /locus_tag="VE170_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16225.1"
                     /translation="QGPEKDGAGKNQASEEPPQGAPPAERFYKAGEGKEGIKGAGYVT
                     VQLPEDLAADSKGQGKPTKAGATGNRARPQVPVSNAPLPAHVPNAPTEKQQMPIEYRG
                     IIR"
BASE COUNT         2068 a         2704 c         2435 g         2017 t
ORIGIN      
        1 atctcgcgat tatagtcggc gagatttttc accggccggc gattgatttc gaccagcagg
       61 tcaccggagc gtaaacctgc ttcttcggcg gcgctccccg gcttcaccgc cgagatgacc
      121 aaaccagcgc cccgttccac accaagagtt tcggccaatt gcggcgtcag cggctggacg
      181 gtcaggccga gttcgccctc ttgcgcggca gcggcgactt cggtttcctt catctcgcca
      241 atggtgagcg acagcggggt ttccttgcca ttgcgaatca ctttgatttg caccgctgct
      301 cccggcacca ctcgcgccac ctgttgcggc aggtcggcgg agtctttgat cggtcggcgg
      361 tcgaactcga cgatgacatc gccgattttc accccggcgt ggtccgccgg cccgccattg
      421 accatgtctg cgaccaaggc gccccgcgca ggattgatgc tcaaagattc ggcaatttcg
      481 ggactgactt tttgtaccga cacaccgatg aaaccgcgca cgactttgcc cttgtcacgc
      541 aattgcggca acagttcttt aacggcattg gaagggatgg cgaaaccgat gccgatattg
      601 ccgccggact ggctaaagat cgcggtgttg ataccaacga cttcgccacg taaattgatc
      661 agcggtccgc ccgaattacc gggattgatc gaggcatcgg tctgaataaa attgtcgtac
      721 ggtccctggc cgatattgcg ccccttcgcg ctgacgatgc cggaggtaac cgtgtggtcg
      781 aggccgaacg gattgccgat ggccatgacc cactcgccga cttccagtcg atccgagtca
      841 cccaaagcta ccgcgggcaa gtcgcgacca gcttcgattt tgatcaccgc catgtcgctc
      901 ttctggtcct taccgactac tttcgcgtca tagtttttgc cgtcagacag tgtcacacta
      961 agtttttgcg cgccgtcgac gacatgatag ttggtcaaaa tcgtgccgtt gccgtcaacg
     1021 atgaagcccg aaccgatgcc gctttgacgc tgcggtcccc ggggtatgcg cccgccaaaa
     1081 aatcgctgcc agaactcttc cccgggatcg ttgtccccaa acggtgaggg tgcttcctgc
     1141 gccgcgccgc ggattctgct tgtcgaaacg ttcaccaccg aaggcccgac cttcttagcc
     1201 aagttgatga agtcgggtag attctgtgcc ggagtagctg cgctggacgc ggcatgggct
     1261 ggcgtgagac caccatcata gtaatgcccg acgatgctca tccctcccat gcccgccaat
     1321 acggcaatgg cacaagcacc aactaatggg gacggttttg atcggcttgg ctgctggtct
     1381 gaaaactgag acatggtttt tttcttcctc cactaacatt agacgcggcg acgaacgatt
     1441 ggttcctgct aagttcccaa gcggtccgcg tctaggggag gttagccggc gagcgactga
     1501 gcacgcgctt tgacgggacg gccctggagc gcggcactct tccagcgcag cagtccagca
     1561 cgcaaatatt gcggatctag accaagaatt tcacaaacat tgtcgaaggc aaataaatgg
     1621 gatcgatcat tgtcgaggat ccactcttcg gcatcgtggt gcatcgcctg cttacgcttg
     1681 caactcgacc cgacgttatc ctgaaaacag ctaaccgcat cgcgcagcac ggcgaacatc
     1741 aagacccgct cggcgtccaa gtgtacccgg cgcgtttggg tcgctaagta ctggctctcg
     1801 atcagcacgt cgggctgaaa gatcgccatc gcgcgctcgt cgagctgcat gcgcggccca
     1861 ttattattgt ctttaaccca aaacggctgg cgctcgtgat tggctttgga tgtagacact
     1921 ttttctcgtt tggcccccat aagacacctc ctctgagtga gttagacgac ccagagttgc
     1981 ccaaggttcc aaagatttga cgatttttga ctagcgaccg catgctgggg gccggctagt
     2041 ccggaagcaa tgtcgctaat caatggccac aacatggctt tcaggtccca actctcgatt
     2101 cctagttgcc attctcgcca gttgacccca ccacgcaagg tgataaatta accggccatg
     2161 ccactagcga ccatcgaaga agcgattgac gagattcgcc aaggccgtat ggtcatcctc
     2221 accgatgaca aagaccgcga aaacgaaggc gacctatgca tggcggccga aaaggtcact
     2281 cccgaagcga ttaacttcat ggcgactcat ggccgggggt tgatctgtct gaccttgacc
     2341 gatgaacggg tccgacattt gggattggcc atgatggtgt cggacaacac ttcgccgttt
     2401 ggcaccgcct tcaccgtttc catcgattcg gcccagggca tcaccaccgg gatttccgca
     2461 gccgaccgcg ccaaaacgat cctcgactca atcgctgatg acgccaagcc ccaggatctg
     2521 gcgacgcccg gtcatatctt tccgctgcgc gcacgcaacg gcggcgtcct cgtccgcgcc
     2581 ggccagaccg agggctcggt cgacctcgcc cgcttagccg gccttaagcc cgccggagtc
     2641 atttgcgaga tcatgaaaga tgacggcacc atggcgcgcc agccggacct catgcgcttc
     2701 gccaaaaagc acaagctcaa agtcgtcacc aacgccgatc tgatccaaca ccggttgaac
     2761 tacgattcgc tggtctatag ggccgcatcg gcgcccctgc caacccgcat cggcggcgag
     2821 tttcaagcca tcgtctacaa tacccacgtc gacgaaagcg agcacttggc gttgatcaag
     2881 ggacagattt cgcccaatga agaaaccctg gtgcgagtgc ataccaaata tatacccggc
     2941 gatgttttcg gcttcgaact gctcaacacc ggcgcggtta ttcagcgctc catggagttg
     3001 atcgccaaag cgggcaaagg tgtgatttta tatttgcagc ccaaaataaa agcgcatcac
     3061 ccagccatgg tcgcctatcc taaagtcgaa ggtaagcagc aaaaaggcat gaatgcgtcg
     3121 ttcgtatatc aagcggattt caaggaatac ggcatcggcg cgcaaatcct gcgcgacctg
     3181 ggcgtgcgca agatgcgcct actgaccaac aacaaaaaac atctcgtcgg tctgcgcggc
     3241 tacggtttgg aagtgacctc attggtgccg atcccaaggg aaaaccccct gcgcgccacc
     3301 accaaacatc ctagagccaa agccaaacat gcctaagcgg atgtaaaatt tgccatttga
     3361 ttccgaagga cgcgaagagt tatgccgatc ttcgaataca cctgccgcga ctgcgaccag
     3421 cagttcgaga cccttgtctt atcgatacgg gataaaatca gctgccccaa atgccatagc
     3481 cagcaagtca acaagcaatt gtcagtcttc gccgcgtcgg cagcgggcgc cgacagcgag
     3541 ccaagcggcg gcgcctgcac cggcacgccg caaagctgcg gctgccgcta gcggcggtga
     3601 ggaaaaaatc acggaccaaa attttagatt tcggtcggtg gcagaaggaa gaatctagcc
     3661 cctattctga acatcgattt tgaatcggca actggctcac cgcacggtaa cggcaactga
     3721 ctacgcggaa tcttcagatg gagtcatata gctcaataaa tcgagacgga tgcgcttgag
     3781 tgcttcgcca acattgccga acacgcgttc ttcaaccaac tgccctttga cccaatagtc
     3841 gagtgacggc gtatcgagaa aattgatgtc gacgtagcgc tcgcccaccg gctcaccggt
     3901 gatcatgacg cgcaccgaat agccggcatc gataacgatg ccgcgcgctc gcggcggtag
     3961 cggcagcgcc gctttgatct tctccagttt gtccggcggg tacttgccga tatcgaggga
     4021 atagtagccc cactggccag gttcgacttt gagctccttg aaaattttca ggttgatatc
     4081 gacaagattc ttcgccgcct catcctgatt gtagttctgc aagaggtcga ctgcccattt
     4141 ctcgatgatg cctctgaccg gtggtgccgc ggacttatcg cgctccttga ccgcacggcg
     4201 gtcaatccac catttggttt cttcgaatgc aggagttttt tcgtcagcca taagtttagc
     4261 ctaactcatg attgcgccca ggcaagttta gatcttgaaa tcctccagcg tgctcttggc
     4321 aaacagcgaa tcgacgtcga gctttttgct gatcaatcct tgttcgtggg tgtattgcac
     4381 taaagtctcg agaatatggc gattggcttc cagaccatag gcccaaggat caggccccag
     4441 ggcttcccgc tccttttcca tctcgtcgat ggaccaagcc atcatatatt taagggcaga
     4501 aaactcgtac atggcttcct ggcagatgcg tttggattcg ttgaaggctt taaacaagct
     4561 ttgggcgatc catggatgtt gttcgtaaaa ctcttcgcgg atgacgatgg tgtgcatgat
     4621 gggaaaaatc ttcgtgcgct tgaaatagtc ggtttccact tcgcgaaagt tcggaatcag
     4681 cctctgcact ttaggatggc ggcgcacaaa cggcgacggc atatgcgcgg agatcatcgc
     4741 atcgatctcg ccgcgctcga gcatgccgga aagcgtctta tcgcccgcga tcggctggat
     4801 atcgatgttg ggtggcagct tatgctgcac cttgtcctgg cgccctggag tctcctcacc
     4861 gccggtaaac caatgcatct tttccggcaa cacaccgtat tcatgttgca agatgccgcg
     4921 cgcccaagtc gccatggtaa tctgatattc gggcacgccg acacgcttgc cagtcatatc
     4981 cgccggtttc ttaatgcccg agtcggtatt gatatagatg catgagtggc gaaaagtctt
     5041 cgacggaaag actggaatac cgatgagtct cggcgagccc ttgtcgcgcg acatgaggaa
     5101 ggaaccggtg gacatctccg cgacatcgaa ttcgccgtag cgcagcatgc gccaaaaagt
     5161 ttcttccact cgcagcggca aataagtcag gtcgacaccc tcgacctcga cgcgcccgtc
     5221 ctgcaatgcc cgcgtgcggt catagtccca gcaagataaa gtcaaacgaa ttttccccat
     5281 gatgaactcc tgtgcgaaaa tattggcgaa gttacgaacc gacgttggcc agaaactgat
     5341 gggcgctgac aacgaactgt tcgcggtcgc tttgcaacac gaccgcggct tcacccgaag
     5401 cgagggttgt tcggcgctga attaagtaga gcttgccttt atagttgacc tgggcgcccg
     5461 gatgatagcg ttcaagatcc aagtgatccg gctggggatc gatcttttcc atgaccgatt
     5521 tctataacat agcgcctggc aagcttaaac cgcccaaggg agttgcgcac ggaaccgcac
     5581 tctaaactat tccagaaccg caggctgtga ggaagtgcag ccttctgacg gtggcagcaa
     5641 agtccggttc catccgccac cccagctcat cacttttcac agagccggct cggcgcacta
     5701 tcgtcaattt actgctcaaa agtagaggct catcggctcg ccgtggatgg gtcgccccca
     5761 ccttggtaga actgagaacg cgcttttcgg ataaattgac aagcttccag gtcaagctta
     5821 cagttcgctc cagattggga ttcccgctat gcgtatcgct gtcatgggca caggtgctgt
     5881 cggcggttat ttcggcgcga aactcgcatc cgcaggccat cagctcgcct tcatcgcccg
     5941 cggtcggcac ttgcaggcac tcaggaaaaa cggcctttgg gtcacgagcc agaatggcaa
     6001 cttgcggatc gacaatgccc agttcaccga cgatacggca acgattggca aggtcgatgc
     6061 gattctgttt tgcgtcaaat cctacgatac cgaagtagcc gccgaacaga tcaaacagct
     6121 ggtgagcgac caaacgctga ttctatccct gcaaaacggc gtcgataatc cggacaaact
     6181 cgctcgtgtt tttgaccccg gtcaaatctt gccggcagtt gtctacgtcg gtgcgcaact
     6241 taccgctccg ggactcgtcg tccatgccaa cggtggccgg ataatctttg gccagatcga
     6301 tgccggcgtc agcgaatcga gtaattcgct gcggcaaatc ctcagcaatg ccggtatccc
     6361 ctgtgagatc agcgccgata ttcagaaaat tcaatggacc aaactgctgt ggaacgcgcc
     6421 cttttgcgcc atcggctgtt tggcccacgc caacgtcaaa cagattgtcg agtctgagtc
     6481 tttgaccgcg cttgcgctcg actgtatgac cgaggtccaa ggcgccgcgc atgcgcgtaa
     6541 catcgatctg cgccgcgaac agttcgatga gatcatgacg ttttcgcgca cactgggcca
     6601 gttcaaaccc tccatgctgc aagatctaga ggcaggcaaa ccgcttgaat acgaagcttt
     6661 caatggcata gtggtgcaga cgctacgaca agccggagcc ccggcgccga tcaatcaagt
     6721 attttatgcc acgctcaagt ttttagatca aagaattcgc gcgcaactca aagcctaagg
     6781 agctttcatg ccgattatac aaagcaaaaa agtcccgcta ttgctttggc tattcttact
     6841 atgcgccacg ccgctggctt acgcccaagg gacgcaaaaa gaattggtcg cccgcgggca
     6901 atatatcttt gccgtctccg gcggttgcgc ttgccatacc gaggtcaagg gcgtccataa
     6961 cgccggcgcg cgcccgtttc ggatcccgct cggcacagtc tacagtacca acctcacctc
     7021 ggacaacgaa accggcctgg gtagttggac cgatcaacag atcattgatg caatcatcaa
     7081 gggcctgcgt aaagacggca gccgacagct gccgctcatg ccttacactt tatactccgg
     7141 catggcacag gaggatttaa aagcgctggt ggcctatctc aggacgctca agccagtcaa
     7201 aaaagcgaca ccggagttga cgacctctac gccctttatg cgcagcgtcg tcaccgagat
     7261 atggctcaaa gccttcggta ttttttacga atcaccggcc caggccccca aaagcggcat
     7321 ccagcgggga aaatatctgg cgaatcatgt cgccatctgc ggcgattgcc acacaccgcg
     7381 aaactcattc ggcgtgccca accaatcgct ctatatggca ggctcgccca aagatggcct
     7441 gatcggcgaa ctggttccta acatcacgcc ggacaaagaa accggcatcg gcgactggaa
     7501 acgcgaagaa atcgcggaat tgttgctaac gggagttaaa cccgactccg ataccgttca
     7561 gggtctcatg gaagaggtga ttcgcggcac gactcacgga tacaaagaca tgaccaaaga
     7621 ggatgtccta gcgatcgccg actacttgaa atcgattcca gcaataaaga acaagatgaa
     7681 ataattctga aatccgcaat tgaaatacgg caaccgactc aagtgacaag tccggagcag
     7741 tgacgccacc cggcgatacg gtcgcagcgt cttatctcct ccactccact cgatcccttt
     7801 ccaaccgagc tgactggacg cagcagaagt ttagtcactg ctcagcgcta accgtgcggt
     7861 aaccacaatt atgctccagt gaagccatgg gtctctagcg attcggacta atttcgcctc
     7921 acctgcttca tcacctcggc gatcacctga tcggcgctga cattctcggc ttcggcctgg
     7981 aaattaagaa tgatgcgatg gcgtaacgcc gggtagatga cgttctcaac atcttcatag
     8041 ctgacgtgca cgcggccgtc agccagcgca ttccccttgg cgccaaggat tatcgcctgg
     8101 gcaccgcgcg gactggaacc gaaacgaata tattgcttga tcgaaccggc cgtgccgccg
     8161 ctttgaccgt tgccttgggt cgcgtgaacc aggcgaacga cataatcttc gatcggcggc
     8221 gcgatgagaa cctgacgtaa tagctgtttc atttcgttga ccagtttacc gccgtcttcg
     8281 ggcaatactt tggcggcatc gccgatttca accccagtgg tgcgtttgag gatttcgcgc
     8341 agttcgtctg gcgccggagg agccactaaa agtttgaaga aaaaccggtc gagctgggct
     8401 tcgggcagcg ggtaggtgcc ctccagttcg atgggattct gcgtcgccag aactatgaac
     8461 ggcgattcga gaatatgcga ctcgccgaaa accgtcacct gcttttcttc catcgcctcc
     8521 aacaccgccg attgagtctt tggagtggcc cgattgattt cgtcggccaa taccacatgg
     8581 gcgaagatcg gccctttttt gaattgaaat tcgcgccggc cattggcctc ggtcagcact
     8641 tcagtaccga cgatgtccga tggcattaaa tccggcgtga actggatgcg tttaaaggac
     8701 atgccgagcg cgtggctcaa tgacttgacg agcagggttt tgcccaaccc cggcacgccc
     8761 tcgagcagca catgaccgcc ggagaaaaac gcgataagca ctttgcggat gacattgccg
     8821 ttgccaacga tgactttctg cacctccgtt tcgagaccct tgaatatttg ttgaaactgt
     8881 tcgagctcca tcgcactccc ttccatcaac gaatgatgcc gcgatattcg attggcatct
     8941 gctgtttttc cgtgggcgcg ttgggcacat gcgcgggcaa tggcgcgtta ctaaccggca
     9001 cctgtgggcg cgcgcgattg ccagtggcac cagccttggt cggtttgccc tggcctttgc
     9061 tgtccgccgc caaatcctcc ggcaactgca ccgttacgta gcctgcccct ttaatgcctt
     9121 ccttgccttc accggcttta tagaatcttt cggcgggcgg cgccccctga ggcggctctt
     9181 ccgacgcttg gtttttcccc gcgccgtctt tctcagggcc ttgg
//
LOCUS       DASQKS010000468         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1512577, whole genome shotgun sequence.
ACCESSION   DASQKS010000468 DASQKS010000000
VERSION     DASQKS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512577"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..1264)
                     /locus_tag="VE170_12100"
     CDS             complement(326..1264)
                     /locus_tag="VE170_12100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16226.1"
                     /translation="MSNFPVIDTDGHVLERQSDIRKYLAEPWAKRTTALWPGDQPWDN
                     DMMDGFEMARNWKGLSAAQQVDNWHKIMDEHGIDKAICFPTGSGNIAKNQEVPYQIAV
                     TRAANTQFAKEYNARSDRLFCVGCLPMRSPKAAAEELRRAVTELGLKGFEILPTGLPL
                     ALGDPYYDPIYEEAEKLNVVLGIHGTRNTSRELGTSGLSTFAEVHSYAFPVGILLQFT
                     SIVCQGLPLRFPKLKLAFLEIGATWLPYYLDRLDEHWEKRAAIEMPLLKQKPSEVVRQ
                     AKLLQRRARRVAIDTHRRLRRRRAFSLRLRHPALGQ"
     gene            complement(1430..2293)
                     /gene="rfbD"
                     /locus_tag="VE170_12105"
     CDS             complement(1430..2293)
                     /gene="rfbD"
                     /locus_tag="VE170_12105"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HYJ16227.1"
                     /translation="MKLLVTGAAGLVGRHVARRMAHGHEVLALEHGDLDITDRAAVRR
                     CLFDEKPALIFNCAVLQVDDSERLPAQAQAVNVAGPAFLAEAANDIGAEIVHFSTQYA
                     FDGEPVGRLPFTIHDEPNPLNNYGKTKVGGEAAVLAACPRSFVVRTSWVYGSGKNSFL
                     CTVHDDLKAGNKVRAIDDIWSSTTYVGDLIERVLKIRATGRYGTYHVVNNGICSYYEF
                     ALEAGHVLGLTRAQIDLVIEITHERDMKRVAARPRYTPLRCLLSEQLGLPPMRDWRAA
                     LAEYVGSGSME"
     gene            complement(2505..2846)
                     /locus_tag="VE170_12110"
     CDS             complement(2505..2846)
                     /locus_tag="VE170_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HYJ16228.1"
                     /translation="MKNAIKSISGLALAATLLAGCAGGSITTREKGLGIGALGGATAG
                     GLIGAAVRRPAAGAIIGTVLGAGAGGLIGDQMQGREIQGNEHDQAIQRNEQEILRQRR
                     ELEDLKQRREY"
     gene            complement(2989..3729)
                     /locus_tag="VE170_12115"
     CDS             complement(2989..3729)
                     /locus_tag="VE170_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ16229.1"
                     /translation="METTMLSVASRRPPAKLRRSWPALVALFCLAPLGLSACLTIASP
                     PSRRAPQDYPQSRTQSYGSRGADANDSERLRRVMMPLIQAMDRPCRLDQTKIGIINQN
                     EINAANAGSCQFYVTMGLLRRASDDQLRGVLAHELAHQDLGHVAKAQVLGATINALSA
                     GLQQLSPLAGVVAPIAGELAARSYGRNEEFSADKHGVDILRRAGYSKVVMVESLAWIR
                     RISGDQGGGFLSTHPELNDRIATIQRLR"
     gene            complement(3757..>4257)
                     /locus_tag="VE170_12120"
     CDS             complement(3757..>4257)
                     /locus_tag="VE170_12120"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYJ16230.1"
                     /translation="RQRENSRAIDTDAKLDTNSKRWAVNRQQNGKATRIYEFDSQNTF
                     DPVTAVYLVRSIDFKVGDKLYFKVFGGRYQYLLELFVEKKEPVELQSGKTVEAYKIVP
                     RVQNLKKSGYASRLTEAAIWISTDERRLPVKLSSKVAFGSVQLDLMEDKRGTQSTASN
                     NNRPAS"
BASE COUNT          887 a         1208 c         1222 g          940 t
ORIGIN      
        1 gtccgtactt ggcgtaaaga ccgcgcttct cggccaatga aaccaacagc gcgtgcggat
       61 tgaccgagct gtaggcaatg cggatggtgt cggccgcgtg agctagtccc gcccaggtcg
      121 cgcaaactag gtatagcgaa atgattcggg taattttctt ggtcatcgat taatttattc
      181 ctttgcgtct ttgcgccttc gcgggaaaaa tatccgaacc acttacaacg aaaacagttc
      241 cttggcattc ttatacaaaa tcttttcctt ggtctcattc gccagctgtt tatgattctg
      301 caactcatgc aggttatgag gaaactcatt gtcccaatgc gggatgtcgg aggcgtagag
      361 aaaatgctcg gcgccgacgt agtcgacggt gtgtgtcaat tgcgactcgc cgggctcgac
      421 gctgaagtag ctttgcctga cgcacgacct cgctcggctt ctgttttaat aacggcattt
      481 cgattgccgc gcgtttttcc cagtgctcat ccaagcgatc caagtaatac ggcaaccaag
      541 tggcgccgat ttccaggaat gcgagcttca atttcggaaa gcgcagcggc aaaccctggc
      601 agacgatgct ggtaaattgc agcaggatac ccacggggaa agcgtaggag tgaacctcgg
      661 cgaatgtcga taaccccgac gttcccaact cccgcgaggt gttgcgcgtg ccgtggatgc
      721 cgagaacgac gtttagtttc tcggcttctt cataaatcgg atcgtaatac gggtcgccga
      781 gggccaacgg caaccccgtc ggcaatatct cgaagccctt gaggcccaac tccgtcaccg
      841 cgcgacgcaa ttcctcggcg gctgctttcg gtgaacgcat cggcaagcag ccgacgcaaa
      901 acagccggtc ggaacgcgcg ttgtactctt tggcgaactg cgtattggcg gcgcgggtga
      961 cggcgatttg atagggcact tcctggttct tggcgatgtt gccggagccg gtgggaaagc
     1021 aaatcgcctt gtcgatgccg tgctcgtcca ttattttgtg ccagttgtcg acctgctgcg
     1081 ccgcgctcaa ccccttccaa ttacgcgcca tctcgaagcc atccatcatg tcgttgtccc
     1141 agggctgatc gccgggccac agtgcggtgg tccgtttcgc ccagggctcc gcaagatatt
     1201 tacggatgtc cgattgccgt tcgagtacat gaccgtcggt atcgatgact ggaaagttcg
     1261 acatgggagg atcctccgtc gcgacgactt aacctctacg cccttttagt cctgagtgaa
     1321 ggagggtgtc aatgcgccaa tagacggaga attgacgtgt tggagtagag cattgatggg
     1381 caaaagcggg acgggtctaa agatcagaca cccaatacgc actactccat tactccattg
     1441 agccactgcc gacgtactct gccaacgcgg cgcgccaatc gcgcatcggc ggcaaaccga
     1501 gttgttccga aagcaaacag cgcagcggtg tgtaacgcgg ccgcgcggcg actcgtttca
     1561 tgtcgcgctc gtgagtgatt tctatgacta gatcgatttg cgcgcgcgtg agtccgagca
     1621 catggcccgc ttcgagggcg aattcatagt acgagcagat gccgttgttg acgacatgat
     1681 aggtgccgta tcgcccggtc gcacggatct tcagcacccg ctcgatcaag tcgccgacat
     1741 acgtcgtgct cgaccagatg tcgtcgatgg cgcgcacttt attgccggct ttgagatcgt
     1801 cgtgaacggt gcagagaaaa ctgttcttgc cgctgccgta gacccaagaa gtgcgcacga
     1861 caaaactgcg cgggcacgcg gcaagtactg ccgcttcgcc gccaacttta gtcttgccgt
     1921 aattgttaag cggatttggc tcgtcgtgaa tggtaaaagg aagccgcccc accggctcgc
     1981 cgtcaaacgc gtattgcgta ctgaaatgaa cgatttcggc gccgatatcg ttggccgctt
     2041 cagccaagaa agctggtccg gcgacattga cggcttgggc ttgcgctggc agccgttccg
     2101 aatcatcgac ttgaagcact gcgcaattga agatcagcgc cggtttttca tcaaacaagc
     2161 aacgtcgcac ggcggcgcga tcggtaatat cgaggtcgcc atgctcgagt gcgagcactt
     2221 cgtgcccgtg cgccatacgg cgcgccacgt gacgacccac caacccggct gcgccggtga
     2281 ccaataactt catcgcccgc caactctaaa acgcggcgaa gaagtcatca agcgcaacgc
     2341 aattgcacgg cggcatcaaa cgacattcaa aggcgtgttt cgctctaaac gcttccggaa
     2401 gtgtaagggg cgatcttctg tcaacgctga gagggaaaag acggagcggg gggcatatgc
     2461 cccccgctgt aagaatctaa ataatatgag atggagattg gtgactaata ctcgcgccgc
     2521 tgtttgaggt cttcgagctc gcggcgctgg cgcaggattt cctgctcatt gcgctggatc
     2581 gcctggtcat gttcgttgcc ttgaatctcc cggccctgca tctggtcgcc gattagacca
     2641 ccggcgccgg caccgagaac cgtgccgatt atcgcccctg cagcgggtct gcgaacggca
     2701 gcgccaatga gaccgccggc agtcgcgcca cccaaggcac cgatgcccaa tcccttttca
     2761 cgcgtggtga tcgagccccc ggcgcatcct gccagtagag tcgccgctag cgccaagccc
     2821 gaaatcgatt taatagcgtt tttcatggtc tccgcctcct tgctggttta gacggaggct
     2881 tcgataaaat gtttacagta aagattcgat ttcagaggga tttggacctt gccgggatga
     2941 tcaaagggca atatagcgaa ttacgccgaa gggttcgtat tcttggtcct accgtagccg
     3001 ttgaatcgtc gcgatccggt cattgagctc cggatgagtg gaaagaaagc cgcctccttg
     3061 gtcgccggaa atgcggcgaa tccaggcgag ggattcgacc atgaccacct tggagtagcc
     3121 cgcgcggcgc aatatatcga cgccatgttt gtcggcgcta aattcttcat tacggccata
     3181 actgcgcgct gccagttcgc cggcgatagg cgccacgacg ccggccaggg gagacaactg
     3241 ctgcagaccc gcgctcaggg cattgatcgt cgcgccaaga acctgcgcct tggcaacatg
     3301 gcctaaatct tggtgcgcca gctcatgggc taaaaccccg cgcaactggt catcggaagc
     3361 gcgccgcaac aaacccatag tcacgtagaa ttggcagctg ccggcattgg cggcgttgat
     3421 ctcgttctga ttgatgatgc cgattttggt ttgatcgagc cggcagggcc gatccatggc
     3481 ctgaatcaaa ggcatcatga cgcggcgcag gcgctcggag tcgttggcat ccgcgccgcg
     3541 tgacccgtaa ctttgcgtcc gggattgcgg gtagtcctgt ggcgcgcgtc ggctgggtgg
     3601 cgacgcgatg gtgagacacg ccgagaggcc caacggagct aagcaaaaaa gcgccaccaa
     3661 cgcgggccag gagcggcgga gtttcgccgg cggccggcgg ctggctacag acaacatggt
     3721 cgtttccatc tgaattccgc ccatgaccaa tttattctaa gatgctggcc gattgttgtt
     3781 gctcgcagtc gactgggtgc cgcgtttatc ttccatcaga tcaagctgca cgctgccgaa
     3841 ggcgaccttg ctgctgagct ttaccggtaa gcgccgctcg tccgtcgaga tccagatcgc
     3901 cgcttcggtt aatctgctgg cgtagccgct cttcttgaga ttctgcaccc gcggcacgat
     3961 cttgtaggct tcgacggtct tgccggactg cagctccacc ggttcttttt tctcgacgaa
     4021 caattcaagc aaatactgat atcgtccacc aaataccttg aaataaagct tgtcgccgac
     4081 cttgaagtcg atactgcgga ctaaatacac cgcggtgacc ggatcgaagg tgttctgcga
     4141 gtcgaactcg tagatcctgg tggctttgcc gttctgttga cggttcactg cccagcgttt
     4201 gctgttcgtg tcgagcttgg cgtcggtatc gatggcccgg gaattttccc gctggcg
//
LOCUS       DASQKS010000469         5605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4036951, whole genome shotgun sequence.
ACCESSION   DASQKS010000469 DASQKS010000000
VERSION     DASQKS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4036951"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..2260)
                     /locus_tag="VE170_12125"
     CDS             complement(221..2260)
                     /locus_tag="VE170_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYJ16231.1"
                     /translation="MRSRRKSKGSNKRAAKKTAFRVEEATITAVHRAFRAKKLTAAEL
                     VSRYFERIRAHNGVCVGGEVDAATGLQLGDITPIPNAGQLNAFITLNLKEDKRIKLGF
                     PEKLKRTHTGRDDAKYPDALDRAWELDRYFARTGKFAGPLHGIPIAIKDNYDTFDMRT
                     TAAAAAQYADDRPPDDATMVAKLRAAGAILIGKTNMDEYAPAGIARSAFGGQTCNPYD
                     TTRIPGGSSGGSAAAVAANLAMCALGTDTSGSVRFPSSCCSLVGLVATQGLVSRDGIV
                     PLSFTRDRGGPICRTVQDTAILLEVLAGYDPKDPVTAACAAQPRLSYARFAGGKSLRG
                     KRLGVLRDLMIEASVADRDPIRVANEAIAAMKKAGATIVDPVNVDNAIADLVPYLEPG
                     WLARNFPAAFPESAKPIDQIVSMAFDHALIPSGARGVNLRMLAAQPRGHEAHYAINRY
                     LRERGDPKFKNLEDMLAMPMFSGSLDNFKRAFSDDAATLDTPAQMDHLVRMQSLRQII
                     LQVMAENSLDALVYAYTTIPPHVILPNRLAKTVETRTEPKILKAGTVMSDPTLVPGEP
                     VLQSDLDTHRGSGSSFAVNLSPETGFPAIVVPAGFTREVYDRVPDDSDPNGSRLEGPK
                     KVELPVALEFLARPFEETTLFAIASAFETIKRHRRPPPGFGELVDGPARTTTGKF"
     gene            2476..2691
                     /locus_tag="VE170_12130"
     CDS             2476..2691
                     /locus_tag="VE170_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16232.1"
                     /translation="MARPLAFYHVINRGQSRLHAEIAWLGKVKIYAYCLMSNDCHLLV
                     STPAAVSSVFLQMKSRSAQEKGSLCTG"
     gene            complement(2734..3981)
                     /locus_tag="VE170_12135"
     CDS             complement(2734..3981)
                     /locus_tag="VE170_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYJ16233.1"
                     /translation="MTDYVVARRLLYTVGKHMKNPLERINLQGRSGHSSATLKPLISF
                     ATFVLIIACLYWGQAVLIPVALAILLTFLLSPVADTLERIALKRLPSVIVVVVLTFSL
                     LGGIGWVVMLQFVSLANELPKYKGNIKQKIADVRGAGKGGALEKVQKTVEEVKSEMQK
                     GSEPTKAKDQRRPVIVQAMESFAFWPEEVAAEPMAETFARAGLVIVLVILMLIQREEL
                     RERLILLVGHGSFTVTNQALEEAGQRISRYLLMQTIINCSFGIAVGFALYLIGLPYAI
                     LWGFLAVVLRFIPYVGPFAAAGMPSVLSLAVFEGWYWPMVVAGVFLVLELLINLVLEP
                     LLYSESAGVSGVGLVVAIAFWTWLWGPVGLVLATPLTVCVVVLGKYVRGMDFIGVLIG
                     DQPARNSNIAAVEESPRPVLVGT"
     gene            complement(4376..5155)
                     /locus_tag="VE170_12140"
     CDS             complement(4376..5155)
                     /locus_tag="VE170_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ16234.1"
                     /translation="MSSAPCPLVKVTKEKITEEEARGLAQGFADKNWTDFKVVRPLSY
                     GGGYTTMCYQTAGASSPTRPLVLYSVEYSMDLKNSTGETRNLCVDQYGFVTEFCGDFA
                     MAGATGPIGPAGPMGAQGPAGATGAQGQLGSVGPQAQASQWSSFRDFLFDFDKSEIRA
                     NETKHISDIAAYMKQYPAVQVGIDGHTDPRGTDAFNQGLSERRVNVISEALINAGVSA
                     ARIKAGAFGESRPKCSAPSESCWQSDRRVEVLISSAQTASR"
BASE COUNT         1213 a         1621 c         1588 g         1183 t
ORIGIN      
        1 gaaaggcata agggcctgag tgttgactat ttacccagtt cgcgggccag cctctcttac
       61 gtttgtttat tccgatgtcc ctggcaccaa ctgtacaacc ggctaatcat cgacggtccc
      121 gatgcagcag ccgccctaac tctttggtcg tgagcctact ccactctcgc gccagctggt
      181 aacagtaagt cgtttaacgg cttcgctctg atcaatccgt ttagaatttt ccagttgtcg
      241 ttctcgcagg tccatcgaca agctcgccga atccgggcgg tgggcggcgg tgtcgtttga
      301 tggtttcaaa agcagaggcg atcgcgaaga gcgtagtttc ctcgaagggg cgagcgagga
      361 attccaaagc aaccggcagc tcgacttttt tcggtccttc caaccggctg ccgttgggat
      421 cgctatcgtc cggcacgcgg tcatagactt ctcgggtgaa tccggccggc acgacgatgg
      481 ccggaaagcc ggtctcgggg ctaagattga ccgcaaagct cgatcccgaa cctctgtggg
      541 tgtcgaggtc ggattgcagc accggctcgc cgggtacaag ggtcggatcg gacatgaccg
      601 tcccggcttt tagaattttc ggctcggtcc gcgtctccac ggttttcgcc aggcggttgg
      661 gcaagatgac gtgcggcggg atcgtcgtgt aggcataaac cagcgcgtcg agactgtttt
      721 ctgccatgac ttggagaatg atctgccgca agctctgcat tcgcaccaga tgatccattt
      781 gcgccggcgt gtccaaagta gcggcatcgt cgctgaacgc tctcttgaaa ttatccaggc
      841 tgccggaaaa catcggcatg gcgagcatgt cttcaagatt cttgaacttc ggatcgccgc
      901 gctcgcgcag gtagcgattg atcgcgtagt gcgcttcatg gccgcgcggc tgcgcggcga
      961 gcatgcgcag gttcacgccg cgggcgccgg agggaatgag ggcgtgatcg aacgccatcg
     1021 acacgatctg gtcgatgggc ttggcggact ctggaaacgc cgcggggaaa tttctcgcca
     1081 gccagccggg ctcaagatag ggaaccagat cggcgattgc gttgtcgacg ttcaccggat
     1141 cgacaatcgt cgcgccggcc tttttcatag cggcgatcgc ctcgttggcg acgcgaatag
     1201 ggtcgcgatc ggcgacgctc gcttcgatca tcagatcgcg cagcaccccc agcctttttc
     1261 cgcgaagcga ttttccaccg gcaaaccgcg catacgaaag cctgggctgc gcggcgcaag
     1321 ccgcggtgac cggatctttg ggatcgtagc cggcgagaac ttccagaagg atcgccgtgt
     1381 cttgcacggt gcggcagatc gggccgccgc ggtccctggt aaatgacagc ggcacgatgc
     1441 cgtcgcggct gaccagccct tgggtcgcga ccaggccgac caggctgcag cacgacgacg
     1501 gaaagcgcac cgagccggat gtgtccgtgc ccaaagcgca catggcgagg ttggccgcca
     1561 ccgccgcggc cgagccgccg ctcgatcccc cgggtatcct agtcgtatca taaggattgc
     1621 aggtctgacc gccgaaggcg ctgcgcgcga ttcccgcggg agcatactcg tccatgttgg
     1681 tcttgccgat aaggatcgcg ccggccgcgc gcagtttcgc gaccatggtg gcgtcatcgg
     1741 gcgggcgatc gtcggcgtac tgcgccgccg ctgccgccgt cgtgcgcatg tcgaaagtat
     1801 cgtagttgtc cttgatcgcg atcgggatgc cgtggagcgg gccggcaaat ttgccggtgc
     1861 gggcgaaata acggtcgagc tcccacgccc gatccagcgc gtcgggatat tttgcgtcat
     1921 cgcgccctgt gtgagttcgt ttcagtttct cgggaaaacc gagcttgatg cgcttgtctt
     1981 ctttcagatt cagagtgatg aaggcattga gctgcccggc gttcgggatc ggtgtaatgt
     2041 ctcccagttg cagtccggtg gcggcatcga cctcgccgcc gacgcagacg ccgttatggg
     2101 ccctgatgcg ctcgaaatag cggctcacga gctccgctgc tgtaagcttc ttggcgcgaa
     2161 acgcccgatg cactgcggta atcgtcgcct cttccacgcg gaaggcagtt ttctttgccg
     2221 cccttttatt tgaacctttt gactttcgcc gcgagcgcat gattcacccc tctccggaag
     2281 aacagagcgt caacctaaca aaccggcgcc gtctggtgca attgcttatt tgaaaagctg
     2341 cgtagggcat cgtagcgcgc aggtaatttc ctgccacggc ttaccgcgcc accgcggcac
     2401 aggccaaagc gaaaaaaagg ggtcaagtct acgtttagct cttgaccgcc gaccgggcat
     2461 aaaataaacg gccgcatggc tcgaccgctg gcgttctacc acgtcatcaa tcgtggccaa
     2521 tcgcgcttac acgctgaaat cgcctggctc gggaaagtta agatctatgc atactgcttg
     2581 atgagcaacg attgccatct actggtgtca acgccggcgg cggtgagctc agtgtttttg
     2641 cagatgaagt cgaggtcggc acaggaaaaa ggctcgctgt gtacgggtta agtcgaagag
     2701 ttaagcgacg ctggataacc cggcggtccg ggatcatgtc ccaactaaca cgggccgagg
     2761 gctctcttcc acagcagcta tgtttgaatt cctcgccggc tgatcgccta tcagcactcc
     2821 tatgaaatcc atccctcgta cgtacttgcc caacacgaca acacagaccg tgagaggggt
     2881 ggcgaggacg agtcctaccg gtccccaaag ccatgtccag aacgcgattg caactacgag
     2941 tccgactccc gaaacgccgg cactctcgct gtagaggaga ggctccaaaa ccagattgat
     3001 gaggagttcc aataccaaaa aaactccggc aacaaccatc ggccagtacc aaccttcaaa
     3061 aaccgccagg ctgaggacac tgggcatgcc cgccgcagca aagggtccga catagggaat
     3121 gaaacgcaat accacagcaa gaaaacccca aagaatggca taaggtagac cgatgagata
     3181 gagcgcgaaa cccaccgcta tcccgaagga gcaattaatg attgtctgca tgagcaagta
     3241 gcggcttatg cgctgacccg cttcttcgag cgcctgattc gtaaccgtaa aactgccatg
     3301 gcctacgaga aggattaacc gttcgcgcaa ctcttcgcgc tgaatcagca taaggatcac
     3361 cagaacaatc acgaggccgg ctcgggcaaa cgtttccgcc atcggctcgg cggccacttc
     3421 ctcgggccaa aacgcgaagg attccatagc ctgcacgatg actggacggc gctgatcttt
     3481 ggcctttgtg ggctcgctac ccttctgcat ttcgctcttc acttcctcaa ccgtcttttg
     3541 gactttctct aaggcacccc ccttacccgc cccgcgcaca tcggcgattt tttgtttaat
     3601 gttacctttg tacttgggca gctcattagc caggctgacg aattgaagca tgacgaccca
     3661 gccaattcct cccaggagag aaaatgtaag aactacgaca acgataaccg aaggaagtcg
     3721 tttaagggct atccgttcca gagtatcagc aaccggactt aacaagaacg tcagtaaaat
     3781 cgccagagcg actggaatca aaacagcttg cccccaataa aggcacgcaa ttatcaaaac
     3841 gaaggtcgcg aaggagatca gaggttttag tgttgcgctg ctgtgaccgc tcctgccctg
     3901 gagattgatt cgctctagtg gatttttcat gtgcttgcct accgtgtaga gcaagcgacg
     3961 cgccacgaca taatccgtca cgaaggggtg tcaataacgc gagagttacc cgacgttttc
     4021 ccggcgtagg cggcaggctc ctattattgt tcaatactag acaaggatga aaacaccgag
     4081 cgcctgcgtg ccaatctgct gaccgaagac gagcaagctc gacgtgatgc tcgaacagct
     4141 atgagggtat ggggaaggtg aaaggatgag ggcgaaatgt tcagcgtcta gcgcctggcg
     4201 caagcccgat gcgagaaagt ggcggggtag aaaaaaaacc ttcatggacg tgagcatcat
     4261 tggcacgcct gcctcgcgaa tacgcaacga cccccgggac cggtgggtcc caggggtcgt
     4321 tggtgttttc tctttggctg aacactggct ctgccagagt gatcgaagct accggttatc
     4381 tactggcggt ttgcgcgcta ctgatcaaca cctcgacgcg gcggtcgctt tgccagcaac
     4441 tctcactggg cgcactgcat ttaggccgcg actcaccgaa ggcgccggcc ttgatcctgg
     4501 cggcggacac gccggcatta atcagcgcct cgctaatgac gttgacacgg cgctcgctca
     4561 gcccttggtt gaaagcgtcg gtgccgcgcg gatcggtgtg accgtcgatg ccgacttgga
     4621 cggctggata ttgcttcata taggccgcga tgtcagatat atgcttggtc tcgttagcgc
     4681 gaatctccga cttatcgaag tcgaacagaa agtctctgaa cgaggaccac tgggacgcct
     4741 gggcttgggg acctaccgat cccaattgac cctgcgcacc cgtggctccg gctggacctt
     4801 gcgctcccat ggggccggcc gggccgatgg gaccggttgc gcccgccatg gcgaaatcac
     4861 cgcaaaattc cgtgacgaag ccgtactgat cgacacaaag attacgtgtt tcacccgttg
     4921 aatttttcag atccatggaa tactcgacgg agtataacac tagcgggcgt gtcggcgatg
     4981 aagcgccggc tgtctggtag cacatggtag tatagccacc gccgtagctg agcggccgca
     5041 ccactttaaa gtcggtccaa tttttgtcgg caaacccttg agctaacccg cgcgcttcct
     5101 cttcggtaat tttctccttg gtaactttga ccagtggaca gggcgcacta gacataaatt
     5161 cgtgcgccct tgcgcttgcc tcacggtgtg aaccgctggg tgtctgagcc aagccgatac
     5221 ccgacagcgc caagacactc gctgccgcaa tcaccggcgt tagaactgat ctcaatctct
     5281 gcataagaag attctccact taaattttac taatctagct ttagcgcaac tgttatgccg
     5341 ccacccggcc ccaggcacag gcggtttttc tcaaacattg cgacttgttc aacggtgaat
     5401 cactaagcag gtgcgacaca agcgtcgaga gcttcgacca aacggtcgga atagatttca
     5461 ccgaaacaag aaaaggttta tctatactgg gtattctgag ataccgagcg ttctgcgcgc
     5521 tcaactaccg ctcgtttccc tcacctcgag caggtcatcg aacaacgcgc gtaatgcaca
     5581 accgctttgc gcagtttctc ggtat
//
LOCUS       DASQKS010000470         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_676693, whole genome shotgun sequence.
ACCESSION   DASQKS010000470 DASQKS010000000
VERSION     DASQKS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676693"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VE170_12145"
     CDS             <1..1279
                     /locus_tag="VE170_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYJ16235.1"
                     /translation="FIVNKVLTLMVPLALTVGTYAGIWWYVVVHSAPAAVSVATPELE
                     PLSGSATPAQSDKPQDLGAVQEKPQELATDEVKPQELGSAEITAESSETARSPDAPPQ
                     EMGSLAAPTTAGGGGKVPERFYIWFWASVALSTLLLAYYYWRMDGEQFQILKELAISV
                     VPLGVLTIVVLVVILLGICTATESAAIGALGALYLAVMVKFPRRCGWWSLLGAVIGLL
                     VGLQRGDIATLVVSGSLGAVFVGTGVPLAIGLPKSPELRRNLQEATFLTAKTTAMVCW
                     LFIGSALFSGVFALHGGQGLIERWVLGLNLSPLGFQLVAQAIIFILGWPLEWTEIIVI
                     FCPIFIPLLSHFNVDPILFGTMVAVNLQAAFLSPPVAMSAFYLKGVAPKHVTLNQIFA
                     GMMPYMLIVCLCLVCMHIWPGMTLWLPEFLYGN"
     gene            1355..2695
                     /locus_tag="VE170_12150"
     CDS             1355..2695
                     /locus_tag="VE170_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008279745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYJ16236.1"
                     /translation="MDLADLHLLSASEAALLMRDGVISSEQLVEACLTHIRAVDDNIQ
                     AWAFLDPEYALTQARALDRQRLEGRPIGPLHGVPIAIKDIFDTADMPTEYGSPVHAGR
                     TPSRDATAVALLRGAGSVIMGKAVTTEFAYLYPGKTRNPHNREHTPGGSSSGSAAAVA
                     AHMVPLAIGSQTAGSTIRPAAYCGVIGFKPSHGLISRHRVSPLSRTLDHVGLFARTID
                     DIALLAEQLVAYDENDPDTHPRARIPFATISAEEPPLAPMFAFIKTPMWQRADDDSKE
                     GFAEIVEQLGAQVEEVELFPSAQDAWQWHQTIMGAEVAHNFEREWQNSRALLSEQLRA
                     QIERGREVRAMDYQRALKQIAPLHDSFVELFEQRYDAILTPAAPSAAPKGLTSTGDPS
                     FCTLWTLCGMPCITLPLLQNSDALPIGVQLVGPRGGDARLLRTARWLSKRMAES"
     gene            complement(2776..3624)
                     /gene="lipA"
                     /locus_tag="VE170_12155"
     CDS             complement(2776..3624)
                     /gene="lipA"
                     /locus_tag="VE170_12155"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HYJ16237.1"
                     /translation="MGRRHPDWIKVKAPGNPNYLRLKKLMRGKNLHTVCEEAHCPNIG
                     ECWGNKTATFLILGDTCTRGCRFCAIAKGKPLALDPEEPRNVGLTVKDLGLDHIVVTS
                     VNRDDLPDGGADHFARTVFWIKNLNPGIRVELLIPDFDGNLDALKTVVGSGIEILNHN
                     IETVPRLYSKVRPGHTYECSVNILKSAKEMRPEVLTKTGMMLGVGETREEVMTTLAEL
                     KENNIDIITLGQYLQPSPKLLKVERYLHPDEFKEFKTAADKLGFRHVESGPLVRSSYH
                     AWSHVN"
BASE COUNT          747 a         1068 c         1106 g          887 t
ORIGIN      
        1 atttatcgtc aataaagttc tgactttgat ggtgccgctg gcgctgaccg tcggaaccta
       61 cgctggcatt tggtggtatg tggtcgttca cagcgcgccg gcggcagtca gtgttgcgac
      121 gccagagctg gagccactat cagggtcggc tacgccggcg cagtctgaca aaccgcagga
      181 cttaggcgcc gtgcaggaaa agccacaaga attggcaacc gatgaagtaa agccccagga
      241 actgggctca gcggaaataa ccgctgagtc aagcgaaacg gcgcgatcgc ctgacgcacc
      301 accccaagag atgggttctc tcgctgcgcc gacgaccgcc ggtggcggcg gtaaagtgcc
      361 cgagcgattc tacatttggt tttgggcttc tgtcgcgctt tcgacactcc tgctcgctta
      421 ttattactgg cgcatggacg gcgagcagtt tcaaattctc aaggaactgg cgatctccgt
      481 tgtgccgctg ggtgtgctga ctattgtcgt gctcgttgtc atactcttgg ggatctgcac
      541 tgccaccgag tcggcggcaa ttggcgcctt gggcgctctc tatctcgccg tcatggtgaa
      601 gttcccgcgt cgctgcggct ggtggagtct tctgggcgct gtcatcggtt tactcgtagg
      661 gctccagcgc ggcgatatcg ccacgctagt cgtttcagga tccctgggtg cggtgtttgt
      721 cggcacggga gttccgctcg ctattggcct gccgaagtcg ccggagctca ggcgcaatct
      781 tcaagaagcg acatttctta cagctaagac caccgcgatg gtgtgctggc tgttcatcgg
      841 ttcggcgttg ttctccggcg tcttcgcctt gcatggtggt caaggattga tcgaacgttg
      901 ggtattgggc ttgaatcttt ctccgttggg ctttcagctg gtcgcccaag cgatcatttt
      961 catactcggc tggccgctcg agtggaccga gatcatcgtc attttctgtc cgatctttat
     1021 tccgctgctg agtcatttca acgtcgatcc gatcctgttc ggcaccatgg tggcagtcaa
     1081 tctgcaggcg gcgtttctct cgccgccggt ggcgatgtcg gcgttttatc tcaaaggcgt
     1141 ggcgcccaaa catgtcacgc tcaatcaaat cttcgccggc atgatgccct acatgctgat
     1201 cgtctgtctg tgtctggtgt gcatgcacat ctggccgggc atgactttat ggctgccgga
     1261 gttcttatac ggaaattgaa gcgttcaaat cgttccagcc gtcgagacag ttcaaaacgt
     1321 ccgattgggc cgaacccaca atgacgtgag tgtcatggat ttagctgatc ttcatctgct
     1381 ctcggcgtcg gaagccgctc tactgatgcg cgacggtgtg atcagttcgg agcagctggt
     1441 tgaagcctgt ctgacccata tccgtgcagt cgatgacaac attcaagcct gggcatttct
     1501 cgatccggag tatgcgctaa cgcaagcgcg cgcgctcgat cgacagcgcc tcgaaggcag
     1561 gccgatcggg ccgttgcacg gcgttccaat cgccatcaag gatattttcg atactgccga
     1621 catgccgaca gagtatggtt cgccggttca cgccgggcgg acaccgtcgc gggacgcaac
     1681 cgcggtggct ttgctgcgcg gcgccgggtc agtgatcatg ggcaaagcgg tgacgaccga
     1741 gtttgcctac ctataccctg ggaaaacccg caatccgcat aaccgggagc atacgcccgg
     1801 aggctcgtcg agcggttcgg cggcggcggt ggcggcgcat atggtgccgc tcgccatcgg
     1861 cagccagacc gccggatcga cgattcgacc ggccgcctac tgcggcgtca tcggtttcaa
     1921 acctagccat ggattgattt cacgtcatcg ggtttcaccg ctctcgcgca ccctcgatca
     1981 tgtcggcctg ttcgcccgga ccattgacga catcgcgctg ctcgccgagc agttggtcgc
     2041 ctacgatgaa aacgatcccg acacccatcc gcgggcgcgg attccgtttg ccactatttc
     2101 cgccgaagag ccgccgctgg cgccgatgtt tgcttttatc aagacgccga tgtggcaacg
     2161 cgccgatgac gacagcaagg aagggtttgc cgagatcgtc gagcagctgg gcgcgcaagt
     2221 ggaagaagta gagctgttcc catccgccca ggacgcttgg caatggcacc agacaatcat
     2281 gggtgccgag gttgcccata acttcgaacg agaatggcaa aacagccgcg cactgttatc
     2341 cgaacaactg cgcgcgcaga tcgaacgggg acgtgaagtg cgcgcgatgg attaccaacg
     2401 ggcgctcaaa cagatcgcgc cgctgcacga cagcttcgtc gagctcttcg aacagcgcta
     2461 tgacgcgatc ctgacgccgg cggcgccgag cgccgcgcca aaggggctta cgtccacggg
     2521 agatccatca ttttgtaccc tgtggacgct gtgtggcatg ccctgcatta ctttgccgct
     2581 actgcagaat agcgacgctc tgcctatcgg cgtgcagctg gtcgggccgc gcggcggcga
     2641 tgccagatta ttacgaaccg cgcgatggct ttctaagcga atggcggaga gttaaagatc
     2701 gtcgagtaga gcggtgtgaa gaaggcgcga tcctccgtca gcgatcaact attttcggat
     2761 cctttattcc cgcacctaat tcacatggct ccaagcatga taggaactgc gcacaagcgg
     2821 gcctgattcg acatggcgaa agccaagttt gtcggcggcg gttttgaatt ccttaaattc
     2881 gtcaggatgg agatagcgct cgactttgag cagcttcggt gacggctgca agtactgtcc
     2941 taacgtaatg atgtcgatat tgttttcttt cagttcggcc agcgtagtca tgacttcttc
     3001 tctggtttcg ccaacgccta acatcatgcc ggtcttggtg agaacttccg ggcgcatttc
     3061 tttggcggac tttaaaatat ttaccgagca ttcatatgta tgcccagggc ggactttgct
     3121 gtacagacgc ggcacggttt caatgttgtg attcaatatc tcgatgccgg aaccgaccac
     3181 ggtcttgagc gcatcgagat tgccgtcgaa gtcgggaatt aacaattcga cgcggattcc
     3241 aggattcaaa ttctttatcc agaaaaccgt cctggcgaaa tgatcggcgc cgccatcggg
     3301 cagatcgtcg cgattgaccg aggtgaccac gatgtggtcc aagccgagat ccttgacggt
     3361 gagaccgaca ttgcgcggtt cttcgggatc cagcgccagc ggtttgcctt tggcgatcgc
     3421 gcagaagcgg cagccccttg tgcaggtgtc gccaagaatt aaaaaggtcg cggtcttgtt
     3481 gccccagcac tcgccgatgt tcgggcagtg tgcttcctca cataccgtgt ggaggttttt
     3541 cccccgcatt aatttcttca gtcgcaggta gttgggatta cctggagctt tgactttgat
     3601 ccaatcggga tgtcttcgac ccatgggatt cacggattca aatcggtcaa gccgtccaaa
     3661 ttgttcaagt cgtggatctg cgcggtcgct tcaaccacat cacgataccc gaagctctcg
     3721 gcgaagtgat ggagaaagcg tactcgaaca tcgctgacgc tttgctcaaa accgagttcc
     3781 ttttgcatcg acgtcacttc agcccaag
//
LOCUS       DASQKS010000471         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4858793, whole genome shotgun sequence.
ACCESSION   DASQKS010000471 DASQKS010000000
VERSION     DASQKS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4858793"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3040)
                     /locus_tag="VE170_12160"
     CDS             complement(<1..3040)
                     /locus_tag="VE170_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HYJ16238.1"
                     /translation="MGYLTDELDGETAPITQKRGRTYFAHGAVRLLDTGDRNVSARVQ
                     GSQVYRVNLTVADGFIDYSCTCPFFTGDLNACKHIWATAMAAELRGCLKKILETEHQR
                     GARSSGDQPSGAPARRTRSAQAQAPNWKQQLQTLRHVLTPAASPFNDPPAPERQLFYL
                     IDADYTLSRQKLTLEVARRERKNNGEWGKLKSQRLYSRELDALTDPADRRITALLFGA
                     RQDLGYSYSSYYDSAPSLFAVPENLWELALPLMCQTGRCLLRSPKLNDQTPLSWDAGP
                     PWELCLTVEPESDGTRYRLAGSLRRDEQVLELSEPVLLLAGGLVFHDGRIARLNDYGA
                     FAWVSILRAPSALSFTVEDSEVFLDELVSIPNRPRLELPAELQFETVAGIPQPRLSLK
                     AGKHNNWGSERLSAELSFDYGGSVVPHQHRGGGFYQKAQRRLVTRDLGAEEKARARLK
                     QLGIRKGWESDRGDLQLAPEQLARVVRALSAEGWHIEAQGSLYRTAAGITMQVNSGVD
                     WFGIDGAAAFGDTQVGLPRLLRAIKQGEQMVRLDDGSLGIIPEEWMTKYGMLAGLGTF
                     EGDGMHFKRSQAGLLDALLASEPAVSFDAAFERVRRELGDFSGVAAVAPAGEFHGELR
                     HYQLEGLGWLHFLQRFGFGGCLADDMGLGKTIQVLALLEARRELRRNDPENSPPPALV
                     VVPRSLIFHWKQESARFTPKLSILDHTGVARLKPGEHFDNYDVVLTTYGTLRSDAVQF
                     KNIRFDYCILDEAQAVKNASTLAAKAVRLLKADHRLAMSGTPVENHLGELWSLFEFLN
                     PGMLGTAAVFGRAGKNPDESTRQVLSKALRPFILRRTKAQVAPELPEKTEQTIYCDLE
                     GNDRKLYDELRDYYRTQLLKNGAGENSGQYKIQVLEALLRLRQAACHPGLIDKKKVSE
                     PSAKVDTLLAQLDQVLEENHKALVFSQFTSLLAIVRSRLDQEKIRYVYLDGRTRDRQA
                     KVEEFQNDPDAKLFLISLKAGGLGLNLHAAEYVYLLDPWWN"
     gene            3343..>3982
                     /locus_tag="VE170_12165"
     CDS             3343..>3982
                     /locus_tag="VE170_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16239.1"
                     /translation="MKTNATRIIDADGHVRETDEEIIEYMSGGYRNRRDAMLYFPLTP
                     HHGWHRSIPANDFRPSDFRVPDWREWVDKLDEGRFELTVLYPTRFMHVGQIGNPTYAV
                     ELCRAYNDYLNDRFLIHDRRFRGMALLPMQDPKAAVKELRRVVKRYGMVGGILPADGL
                     QLPLGHKQYWPVYQEADRLGCTLAVHSCNSLRDNDRYLVPNEAATLTHVIPQM"
BASE COUNT          782 a         1229 c         1190 g          781 t
ORIGIN      
        1 gattccacca gggatcgagc agatagacat actcggcggc gtgcaggttc aagcccaggc
       61 cgccggcttt caagctgatc aggaacagtt tggcgtcggg atcgttttga aactcttcga
      121 ccttggcttg gcggtcgcgg gtgcggccgt cgaggtagac gtagcgaatt ttttcttgat
      181 ccaggcggct gcgcacaatc gccaataagc tggtgaattg agagaacacc agcgccttgt
      241 gattttcctc gagcacctgg tcgagctggg ccaacagcgt gtccactttg gcactgggtt
      301 cggagacttt tttcttgtcg atcaagcctg gatggcaggc cgcttggcgc agccgcagga
      361 gcgcttcgag tacttggatt ttgtactgcc cggagttttc gccggcgccg tttttcagca
      421 gttgcgtccg gtagtagtcg cgcagctcgt cgtaaagttt ccggtcgttg ccttcgaggt
      481 cgcaatagat cgtctgctcg gttttctccg gcagctcggg ggcgacttgc gctttggtgc
      541 ggcgcagaat gaacggccgc agcgccttgc tgagcacttg gcgcgtgctc tcgtcgggat
      601 tcttgccggc gcggccgaac accgccgcag tgccaagcat gccgggattg aggaattcga
      661 aaaggctcca cagctcgccc agatgatttt ccaccggcgt gccgctcatt gccaaacggt
      721 gatcggcctt gagcaagcgc acggctttgg ccgccagggt gctggcgttt ttcaccgcct
      781 gcgcttcgtc gaggatgcaa taatcaaaac gaatattttt gaactgcacg gcgtcgctgc
      841 gcagcgtgcc gtaggtcgtc aaaactacgt cgtagttatc gaaatgctcg ccgggcttga
      901 gccgcgcgac gccggtgtga tcgagaatgc tcagcttggg cgtaaagcgc gccgactcct
      961 gtttccaatg aaatatcagc gaacgcggca ccaccaccaa ggccggaggc ggcgagtttt
     1021 ccgggtcgtt acggcgcaat tcccggcgcg cttcgagcag cgccaggact tgaatggttt
     1081 tgcccaaccc catgtcgtcg gccaggcagc cgccaaaacc gaagcgctgg agaaagtgca
     1141 gccagcccaa accttcgagc tgatagtggc gcagttcacc atggaactcg ccggcgggcg
     1201 ccaccgccgc gacgccggaa aaatcgccca gctcccggcg cacgcgctcg aacgccgcgt
     1261 cgaaagacac cgccggttcg ctcgccaaca gcgcatcgag caggcccgct tgggagcgtt
     1321 tgaagtgcat gccgtcgcct tcgaaagtgc cgaggccggc gagcatgccg tatttcgtca
     1381 tccactcttc gggaatgatg ccgagcgaac cgtcgtccag ccgcaccatc tgctcgccct
     1441 gcttgatcgc ccgcagcaag cgcggcaagc cgacctgggt atcgccgaat gcagcggcgc
     1501 cgtcgatgcc gaaccaatcg acgcccgagt tcacctgcat ggtgatgcca gcggcggtgc
     1561 ggtaaaggct accctgggct tcgatgtgcc aaccttcggc ggacaacgcc cgcaccaccc
     1621 gcgcgagctg ttcgggcgcc agctgcagat cgccgcggtc ggattcccag cctttgcgaa
     1681 tgcccaattg cttgagccgt gctcgcgctt tttcctccgc gccaagatcg cgggtcacca
     1741 ggcggcgctg cgccttctga taaaagccgc cgccgcgatg ctgatgcggc accaccgagc
     1801 cgccatagtc gaacgacagc tccgcgctaa gccgctcgct gccccaattg ttatgcttac
     1861 cggcttttaa cgatagccgc ggctggggta tgccggcgac ggtttcgaac tgcaattcag
     1921 cgggtaactc caggcgcggc cggttgggga tcgataccag ctcgtcgaga aacacctcgc
     1981 tgtcttcgac cgtgaaagac agcgccgacg gcgcgcgtaa aatcgatacc caggcaaacg
     2041 cgccgtaatc gttcaagcgc gcaatccgcc catcgtgaaa caccaaaccg ccggcgagca
     2101 gcaagactgg ctccgacaac tccaacactt gctcgtcacg gcgcaaggag ccggcgagcc
     2161 gatagcgcgt gccgtcggac tcaggctcca ccgtcagaca aagctcccag ggcggaccgg
     2221 cgtcccagct gagcggcgtc tgatcgttca acttcggact gcgcaacaga caccgtccgg
     2281 tctggcacat cagcggtaac gccaattccc acaaattttc cggcacggcg aataacgacg
     2341 gcgcggaatc gtaataggac gaatagctat agcccaagtc ctggcgggcg ccgaacagca
     2401 aggcggtgat gcggcggtcg gcgggatcgg tcagggcgtc gagctcgcgc gagtagagcc
     2461 gttggctttt gagcttgccc cattcgccgt tgttcttacg ctcgcgccgg gccacttcga
     2521 gggtcaattt ttgccggctc agggtgtaat cggcatcgat caaatagaac agttggcgct
     2581 ccggcgccgg cgggtcgttg aacggagaag ccgccggggt caatacatgc cgcagggttt
     2641 gcagctgctg tttccaattg ggcgcctgcg cctgagccga acgcgttcgg cgcgccggcg
     2701 cgccggaggg ctgatcaccg gaactgcggg caccgcgttg atgctcggtc tcaagaatct
     2761 tcttcaaaca accgcgtagc tccgcagcca tcgcggtcgc ccagatatgt ttgcaggcgt
     2821 tgagatcgcc ggtgaaaaac ggacaggtac aagagtagtc gataaagccg tcggcgaccg
     2881 tcaaattcac tcgataaacc tgcgagccct gcactctggc gctgacattg cgatcgccgg
     2941 tgtcgagcag acgcactgcg ccgtgggcaa aataggtacg cccgcgcttc tgggttatgg
     3001 gcgcggtttc gccgtcgagt tcgtcggtca gatatcccat gtcgaactga taagggtaac
     3061 gcacgcatcg agagaagtct atgtttgtcg ttacttactc aacgactggc ggcatatttg
     3121 gcttaaagag ctctggctgc caagggaaag aaactgtttc gccacgaagg cacgaaggac
     3181 acgaacagtt gagccaatca atgctccgaa cttcgtggtc ttcgtgtgct tggtaatgag
     3241 atccgctcgt cccatcatgt cgtcgcagtc gaataccggc tttcaattgc aactcagcgc
     3301 cgcgcgtgcg acaaaatctt agaacgattt cctggaggcg gcatgaaaac caacgcaacg
     3361 cggatcatcg acgccgacgg ccatgtgcgc gagaccgacg aagaaatcat cgaatacatg
     3421 tccggcggct accgcaatcg gcgcgatgcc atgctgtatt tcccgctgac gccgcatcat
     3481 ggctggcacc gctcgattcc cgccaacgat tttcgccctt cggacttccg cgtgcccgac
     3541 tggcgcgagt gggtcgacaa gctcgacgaa ggtcgatttg aattgaccgt gctctatccc
     3601 acccgcttca tgcatgtcgg ccagatcggc aatccgacct acgccgtcga actgtgccgc
     3661 gcctacaacg attatctcaa cgatcgtttc ttgattcacg accgccgctt ccgcggcatg
     3721 gcactattgc cgatgcagga tccaaaggct gcggtcaagg aattgcgccg ggtggtcaag
     3781 cgctatggca tggtcggcgg tatcctcccc gccgatggct tgcagctgcc cctcggccac
     3841 aagcaatact ggccggtcta ccaagaggcc gatcgcctcg gctgcacctt ggcggtgcat
     3901 tcctgcaact cgctgcgcga caacgaccgc tatctggtgc ccaatgaagc ggcgacgttg
     3961 acccatgtca tcccgcagat gc
//
LOCUS       DASQKS010000472        10001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_678909, whole genome shotgun sequence.
ACCESSION   DASQKS010000472 DASQKS010000000
VERSION     DASQKS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678909"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VE170_12170"
     CDS             complement(<1..373)
                     /locus_tag="VE170_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYJ16240.1"
                     /translation="MEDRGLKDKDSLQIETPLEPELPICDAHHHLWERPPHGYLLDDL
                     LLDLGAGHNIVSTVAIECRYGYRKGGPEELQPVGEVEFLEAIASQVAANPAITTRIAA
                     AIVGHANLILGDAVAPVLEAQL"
     gene            complement(396..1715)
                     /locus_tag="VE170_12175"
     CDS             complement(396..1715)
                     /locus_tag="VE170_12175"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16241.1"
                     /translation="MPGDKESLRDSLDAEAAGSAAQPKRDPLAWLYRHRAAEILRYRD
                     FRLLWFGHVFGSMSFWMDQVTRGWLIYELTNSTVQLGMIRGVQAIPILFLSPIAGSVA
                     DRYSRKKQILITQIADGVMLAVLALLILYGAVEPWHVYVSAFGMAVVQTFHQPARAAI
                     MTDTVPASHLTNAIGLNSMIFNVARSLGPALAGVLISWSGTGGAYTVQAIFYLLATFW
                     TLRLSDVEAGAARTGGHDAHRFSLGSSIVEGWKFSWQNENVRTALLIAMLAAFFIVPF
                     TTLLPVFARDILQVGASGQGLLLTAMGVGALGSAVMITGFGDRMPRGKIMLGGAAVYG
                     LGAAAFAFSPWFQLAMFLMIIIGLANVCTHALVQTVIQTYSPPQFRGRTIALFHMSQV
                     VMTVGSMFIGSLAALSGTQWAVVLMGSAGALAMLGIHILLPRAWHIR"
     gene            complement(1886..2473)
                     /locus_tag="VE170_12180"
     CDS             complement(1886..2473)
                     /locus_tag="VE170_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16242.1"
                     /translation="MQNNIITKSPSLENSGGGADHDYSRLLRVVGQELETKGISTCCL
                     WMEATRIDVIGLCTGNGGVVEAGFWQRLKSKSAVAGYTTTELRFSIEGLLRLDELNRA
                     KRTLASGRADFLKLSEQLRTVGAIVDRRGGQLIRLDRTAYEGMISSFTVQYRMATGEL
                     VTEEFSASSLYDFSVQIFMMEKSRSSNSRPVSRAA"
     gene            complement(2848..4092)
                     /locus_tag="VE170_12185"
     CDS             complement(2848..4092)
                     /locus_tag="VE170_12185"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16243.1"
                     /translation="MWFNWLVQFKIFEALRHPQYRLLWISHVFSAMGTWMDQVSRGWL
                     IYELTNSSFQLGLVRGVQALPILLLSPIAGSTADRYSRKSQVMIALVVDAFFYGLLAL
                     MIFTGSVKPWHVYASAICMASVWTFQQPSRAAMVSDSVPPDSLTNAIGLNAMMFNVAR
                     ITGPALAGLIIAGWSSGGAYAVQALFYILAAIWNVKLRDDGFVLSSSHGDGDHDEGFI
                     QSIVEGWKFSWHNVEVRTGLLVVIWASLFIIPFNTLLPVFARDVLGVGAKGQGLLLTS
                     MGAGALASSVLIAALGDRMPRGIVMIGGVGVYGLLVAVFAMSTWFSLSMVLMAVMGLC
                     HVASHALVQTVIQAYSPREFRGRTMALFHQTHVILMAGGLIIGALASAIGAPWAAALM
                     SIAGTLSMLALYLFSPRARQIR"
     gene            complement(4278..5555)
                     /locus_tag="VE170_12190"
     CDS             complement(4278..5555)
                     /locus_tag="VE170_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16244.1"
                     /translation="MDETGSTPSEKLAWPIALLVQLRAFEALRHRDYRLLWYGQVFGS
                     MGTWMDQVTRGWLIYELTDSALQLGLVRGIQAIPFLLLSPIAGSAADRYPRKVQVVGA
                     QFASGFLFAVTAALVLGGLIRPWHVYVTAFLMACVQVFLQPSRSAMISDFVPQKTLTS
                     AIGLNAMVFNTARSTGPALAGLLISHFSTGISYAVQALFMILATVWTTQLSQVAPGAP
                     NSHGGHAESFGESIIEGWKFSWRTPEVRTGILIVVVAALFMIPFSTLLPVFARDLLQV
                     GARGQGLLLTAMGIGALCSSILIASVGDRLPRGLLMLGGVALYGVLVVIFSLCHWFPL
                     SMGLMAIIGLCHVSSHALVQTVIQTYSPPEFRGRATAIFHMTQVLLLVGGMLVGALSA
                     VLGAPWAASSMAIAGTLCMVAIFLAVPRARDIR"
     gene            complement(5943..6587)
                     /locus_tag="VE170_12195"
     CDS             complement(5943..6587)
                     /locus_tag="VE170_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16245.1"
                     /translation="MLQWKTLLLGLVWFLMLAFGAVNFSHGQVGQSQLVNPDLAGEKD
                     LLALPHMNATVVKSILEQRPFLKMADLHALLSKTLNKEQLAELYGKMFVQINLNTASD
                     EELLLIPGAGSRITREIRDKRPYKTLAQFRTEIGKSADPKEAARLEQYVFVPINLNSA
                     SDDDILSVPGAGKRMVREFKEYRPWTTFEQFRKEIGKYVDKKEVARLEKYVTLN"
     gene            complement(6589..6711)
                     /locus_tag="VE170_12200"
     CDS             complement(6589..6711)
                     /locus_tag="VE170_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16246.1"
                     /translation="MIGAKAPPLLAPGSMVQLGLLGLAYTYKHPALKSSQRKGE"
     gene            complement(6715..7065)
                     /locus_tag="VE170_12205"
     CDS             complement(6715..7065)
                     /locus_tag="VE170_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16247.1"
                     /translation="MTNPESIVGVEGWRLSRLRKILLSILALGMIGSLTELILLKHSE
                     DLYQWIPLVLLGFGLPMLAWQGALGGVLSLRLLRWLMCGFVAAGIAGVYFHFQGSVEF
                     RLESQPKLAGMELF"
     gene            7307..7552
                     /locus_tag="VE170_12210"
     CDS             7307..7552
                     /locus_tag="VE170_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16248.1"
                     /translation="MNRHLVQGIIVGGLFALPITWIAASDALSAGSFPEILVSVIFGL
                     AAGLCIGALIAANFAMLALEEKEHAVARRRVDAHAHA"
     gene            7900..8934
                     /locus_tag="VE170_12215"
     CDS             7900..8934
                     /locus_tag="VE170_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16249.1"
                     /translation="MKKYLAKRKKTSRGLLVASASPSVAAAPPPVLKEAGSFFVNGEV
                     VQTDNPGGGNPGGRIVVNQMYVEYAIPQKQKAGAWPVIMVHGSGHTGKTYDTTPDGRD
                     GWKQHFLRKGYSVYVVDQVGRARSGWNTEPINNAETKGEATLIPVGGIVQFTYERAWS
                     IFRFGPKPDEWWADSKFPKDCIDQYMAQLVPNTEATLAQPPRKTVDALIALMEKIGPS
                     ILMVHSQSGVYGALTAVARPDLVKGFVNVEGRTGCDLDPQQIKIIAKVPMLVVAGDHE
                     WNGEEHCRKTVAAINTAGGKSTFMGTYERDVRGNTHMMMMDSNNLQVAEWILAWVEKN
                     VPKKSQVSKR"
     gene            complement(9091..9810)
                     /locus_tag="VE170_12220"
     CDS             complement(9091..9810)
                     /locus_tag="VE170_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYJ16250.1"
                     /translation="MGQILPDGLLVHLGRKDLMVKIRGYRVEIEIEMTLTQHPAVKDA
                     GVTAWERDNGDKYLTAYIVSRGPNHATIDQLRMFLRDKLPGYMVPSTFMFLDSLPVTN
                     GKLDRRALPEPSDKRPELSTAYALPTDEIEKTLASIWGKVLDVRPVGIHDNFFDLGGH
                     SLSATQVISQVIKQFQLELPLHSLFQSPTVAMMAAVIRQNQAKRASQAELPQMLREVE
                     AMTEQEAQRRLDEIKSAIAKT"
BASE COUNT         2378 a         2975 c         2741 g         1907 t
ORIGIN      
        1 ccaactgggc ctcgagcacc ggcgccaccg cgtcgccaag tatgaggtta gcgtggccga
       61 caatcgctgc cgcgatgcgc gtagtgattg ccggattcgc ggcgacctgg ctggcaatcg
      121 cttcgagaaa ctcgacttcg ccgaccggct gcagttcctc cgggccacct tttcgatagc
      181 cgtagcggca ctcgatcgcc actgtcgaaa ctatgttgtg gccggcgccc aagtccaaaa
      241 gcaaatcatc cagcaaataa ccgtgcggcg gccgctccca gagatgatgg tgcgcatcac
      301 agatcggcag ctcgggttcc aatggggtct cgatctggag cgaatccttg tctttcaatc
      361 ctcgatcttc caagggtcta tcctcgattt gcatattagc gaatatgcca ggcgcgcggt
      421 aatagaatgt gaatacccag catcgccaac gcgccggcgc tgcccatcag caccaccgcc
      481 cactgcgtgc cggaaagcgc ggcgagggat ccgatgaaca tgctgccgac ggtcatcacc
      541 acctggctca tatgaaacag cgcgatggta cggccgcgga attgcggcgg cgaataggtt
      601 tgaatcaccg tctgcaccag cgcgtgggta cagacattgg ctaagccgat aataatcatc
      661 aagaacatcg ccagctgaaa ccacggcgaa aacgcgaagg cggcggcgcc gagtccataa
      721 accgccgcgc cgcccagcat gattttgccg cgcggcatgc gatcgccgaa gccggtgatc
      781 atcacggcgc tgccaagcgc gccgacaccc atcgccgtca gcagcaaacc ttggccggag
      841 gcgccgactt gtagaatatc gcgggcaaac accggcagga gcgtcgtgaa cggcacgata
      901 aaaaacgccg ccagcatggc gatcaatagc gccgtgcgga cgttttcatt ttgccaactg
      961 aacttccaac cttcgacgat gctgctgccc aaggaaaacc gatgcgcgtc atggccgccg
     1021 gtgcgggcgg cgccggcctc gacatcgctc aagcgcagag tccaaaaggt cgcgagcaaa
     1081 taaaaaatcg cctggacggt gtaggcgccg cccgtgccgc tccaagagat cagcacgccg
     1141 gccagggccg ggcccaggct gcgcgcaaca ttgaagatca tcgagttgag gccaatggcg
     1201 ttggtcaaat ggctcgccgg caccgtatcc gtcatgatcg ccgcccgcgc cggctggtga
     1261 aaagtttgca ccacggccat gccaaacgcg gagacgtaga catgccaagg ctcaaccgca
     1321 ccgtaaagta tcaacaacgc caacaccgcc aacatgactc cgtcggcgat ctgggtgatg
     1381 aggatttgct tcttgcgcga gtagcggtcg gcgacgctgc cggcgatggg cgagagaaac
     1441 aggatcggaa tcgcctggac gccgcggatc atgcccaact gcacggtgga attggtcagt
     1501 tcatagatca gccagccgcg cgtcacctga tccatccaaa acgacatgga accgaacaca
     1561 tggccgaacc acagtaagcg gaaatcgcgg tagcgcagaa tttcggcggc acgatgtctg
     1621 tataaccaag ccaacgggtc ccgcttgggt tgcgccgcgg aacccgcggc ctcggcatcg
     1681 agagaatcgc gaaggctttc cttatcgcct ggcacggagc tcagaaccga ctcccgaacg
     1741 agtgacgaaa acgcatgatc ttttggctat agcacgagcg aaatgatcgc gtccagcggc
     1801 gagaacgaac cgaaagcagt gaggatctcg gcacggccga gatatttggg ataatgtgaa
     1861 aactagcgat cgagcaagat acgaattaag cggcccggga gaccggtcga ctgttgctcg
     1921 atcggctttt ttccatcatg aagatctgca ccgaaaaatc gtaaagactc gaggcgctaa
     1981 attcttccgt taccaactca ccggtggcca tcctgtactg cacagtgaag gacgaaatca
     2041 tgccttcgta agctgtgcgg tctaggcgga tcaattggcc gccgcggcga tcgacaatgg
     2101 cgccgacggt gcgcagttgc tcggagagct tgaggaaatc ggctcgcccc gaggcgaggg
     2161 tacgcttggc tcggttaagt tcgtcgagcc gcaacaaccc ttcgatggaa aagcgcaact
     2221 cagtggtggt gtaacccgcg acggcgctct tcgatttgag gcgctgccaa aaacccgcct
     2281 caacgacgcc gccgttgccg gtgcacaggc cgataacatc gatacgggtc gcttccatcc
     2341 acagacaaca ggtggagatc cccttggtct cgagttcctg tccgaccacg cgcaggagcc
     2401 gcgagtaatc gtggtccgca ccgccgccgc tgttctcgag actcggcgat ttcgtgatta
     2461 tgttgttttg catttccatt tttgtcttct ccgctctcac aaattcaaat cggtgcaccc
     2521 ggcaataact ttaaccgcgc agagaaacga tctcggcaat gaagttcacc cgcgccagag
     2581 ttccgccacc gtcgaaaagt tgacggcacg gcagcgccat tttaaccgtg gagaaaaagc
     2641 cggtgcgagg acacccaagc gggcgatgac agagttcatg cgcatgagcc gatccgaggt
     2701 gctgggttca cggcgctttt cgggccagcg cggggattgc tcgccactta caggcagtca
     2761 ccgttttctt cacgtggcgc gtcttgtgcc gcgcaaaaaa aatatggcac cgtcaggaat
     2821 ttggcgtcag tgaaatagct aggtaggcta acgaatttgc cgcgcccgcg gactgaacaa
     2881 gtagagagcg agcatggata acgtgccggc gatgctcatc agcgcggcgg cccatggcgc
     2941 gccaatggcc gaagccagtg cgccaatgat caagccgccg gccatcaaga tcacatgggt
     3001 ctggtgaaac agcgccatgg tgcggccgcg aaactcgcgc ggcgaatagg cttgaatgac
     3061 cgtctgtacc aacgcatggg acgccacatg acacaacccc ataaccgcca tcagtaccat
     3121 cgacagcgaa aaccacgtcg acatggcaaa gaccgcgacc aacaggccgt acacgccgac
     3181 gccgccgatc atcacaatac cgcgcggcat gcggtcgccc agggcggcga tcagcaccga
     3241 gctggcgagg gcgccggcgc ccatcgacgt cagcagcaat ccttgtccct tcgcgccgac
     3301 gccgagcacg tcgcgcgcga acaccggcaa cagcgtattg aacggaatga taaacaaaga
     3361 cgcccagatc accaccagca gcccggtgcg aacctcgacg ttgtgccaac tgaacttcca
     3421 gccttcgacg atgctttgaa taaacccctc gtcatggtcg ccgtcgccgt ggctgctgga
     3481 caagacaaat ccgtcatcgc gcaatttgac gttccagata gccgccaaaa tgtaaaacag
     3541 cgcctgaacc gcataggccc cgccgctgct ccagccggca atgattagac ccgccagcgc
     3601 cggaccggta atgcgcgcga cgttaaacat catggcgttg aggccgatcg cattggtcag
     3661 ggagtccggc ggcacggagt cggaaaccat tgccgcccgc gacggctgct gaaacgtcca
     3721 caccgaagcc atgcagatcg cgctggcata aacatgccag ggcttcaccg agccggtaaa
     3781 gatcatcagc gccaacaagc cataaaaaaa cgcatctacc accaacgcta tcatcacctg
     3841 cgacttgcgc gagtagcggt cggcggtgct gccggcaatc ggcgatagca gaaggattgg
     3901 cagtgcttgc acaccgcgca ccagtcccag ttgaaacgaa gaattggtca gctcgtagat
     3961 cagccaaccc cgcgacactt gatccatcca ggtgcccatc gccgaaaaca catggctgat
     4021 ccacaacaag cggtattgcg gatggcgcag cgcttcgaag attttgaatt ggacaagcca
     4081 gttaaaccac atggtaaatt tgtcgaaaga ctccgacgcg gaaagaaccg cgatcggacc
     4141 aactcactag ccactcaaag ggtctgaaca gttgctctgg caaaggtccg atacattcct
     4201 atcggcaaac ccgggccggc ggcacgcatc acagtcgccg cccattcgct cgcgacgccg
     4261 tgccatcgac cgcctggcta tcgaatgtcg cgcgcccgcg gcaccgctag gaagatcgcc
     4321 accatacaca gcgtgccggc gatggccatg gaggatgccg cccaaggcgc gccgagtacc
     4381 gcggaaagcg cgccgacgag catgccgccg accagtaata atacttgggt catgtggaaa
     4441 attgcggtgg cccgaccgcg aaactccggc ggcgaatagg tttgaatgac ggtctgcacc
     4501 aatgcatggg aactgacatg gcataaaccg atgattgcca tcaatcccat agataacgga
     4561 aaccaatggc acagagaaaa gatcaccacc agcacgccgt aaagcgcgac acctccgagc
     4621 atcagcagac cgcgcggcaa ccggtcaccc accgaagcga tcaagatcga gctgcataaa
     4681 gcgccgatcc ccatggccgt gagcagcaag ccctgcccgc gcgctccaac ctgaagcaaa
     4741 tcccgggcga agactggcaa cagcgtgctg aatgggatca taaacaaagc ggcaacaact
     4801 acgatcaaaa tgccggtgcg tacttccggt gtgcgccagc tgaatttcca gccttcgata
     4861 atgctctcgc cgaaggactc agcgtgcccg ccatggctgt tcggcgcgcc cggcgccacc
     4921 tggctcaatt gcgtggtcca aactgtggcg agaatcataa atagcgcttg caccgcgtag
     4981 gaaatcccgg tgctgaaatg agaaatcaac aatcctgcaa gcgccggtcc ggtactgcgc
     5041 gccgtattga acaccatggc gttcaaaccg atggcgctgg ttaaagtttt ttgcgggaca
     5101 aaatcggaaa tcatcgccga gcgcgagggt tgtaagaaca cctgcacaca agccatcaaa
     5161 aacgccgtca cataaacatg ccacggccga atcaaaccgc caagcaccag cgccgccgtc
     5221 accgcaaata ggaaaccgct ggcgaactgc gcgcccacca cctgaacttt gcgcggatac
     5281 cgatccgccg cgctaccggc gatgggcgac aataataaga atgggatcgc ctggatacca
     5341 cgcaccaagc cgagttgtag agccgagtcg gtcagttcat aaatcagcca gccccgcgtc
     5401 acctggtcca tccaggtgcc catggaaccg aaaacctgac cataccagag caaacggtaa
     5461 tcgcggtggc gcagcgcttc gaaggcgcgc agctgaacta aaagtgctat cggccaagcg
     5521 agtttttcac tgggagttga gccggtttca tccatcggcg gtttggcgtt gcatcgacga
     5581 gttcgaaaca ttcatagaag tgcgtatcga ccggaaacga caggaaaaat cgcagtccca
     5641 acgcgaggcg tcgactgtaa cacgggtaag gtagagcgtc caatctttga cgagaaatta
     5701 ccggcattgg acttgccgcg tttcgggcaa actggcaagg accgtgctga caaccgcgcg
     5761 tcgttagttt cagctgaccg gcgacatcgt tgtccaatgc ggaaattcac agggaatctg
     5821 gcggggcgaa gcagcagaga tgtgtcgctg cgcaaacgcc aactggccaa ctatcatcgc
     5881 gcgacgacgt cggaaaatcg tccggcgatc tgcgccgaat ccacaagatc gcgaagcgtc
     5941 gcttagttga gcgtgacgta cttctcgagt cgcgccactt ctttcttgtc gacgtactta
     6001 ccgatctctt tgcggaactg ttcaaaggta gtccaggggc ggtattcttt aaactcgcgc
     6061 accatacgtt taccggcgcc cggaaccgat agtatgtcat catcgctggc gctgttaaga
     6121 ttgattggca cgaaaacata ctgttccaag cgcgccgcct ctttggggtc ggcacttttg
     6181 ccgatctcgg tacggaactg cgccagggtt ttgtagggac gcttgtcgcg gatctcgcgc
     6241 gttattcggc tgccggcgcc tgggatcagc agtagttctt cgtcgctcgc ggtgttcaaa
     6301 ttgatctgga caaacatctt gccgtacagc tcggccaatt gctctttgtt caatgtcttt
     6361 gacagcagcg cgtggagatc ggccatttta agaaacggcc gctgttccag gatgcttttg
     6421 accaccgtcg cgttcatatg aggcaaggcg agtaaatctt tttcgccggc caggtcggga
     6481 ttgaccagtt gcgattggcc gacctgaccg tggctaaaat tcacggcgcc gaaagccagc
     6541 ataagaaacc aaactaaccc gagcaacagc gttttccact gcaacatatt attctccttt
     6601 tcgctgcgaa cttttcagcg ccggatgttt ataagtataa gccaagccta gaagtcccaa
     6661 ctgcaccatc gaacccggcg ccagcagcgg cggcgccttg gcaccgatca ccgcctagaa
     6721 aagctccatg cccgccagtt tcggttggga ttccagccta aactcgacgc tgccttgaaa
     6781 gtgaaaatag acaccggcaa tgccggcggc gacaaagccg cacatcaacc agcgaagcaa
     6841 gcgaagacta agcacgccgc ctagcgcacc ctgccaggcc agcatcggca aaccaaagcc
     6901 aagcagcacc agcgggatcc actgataaag atcctcgcta tgcttgagca agatcagttc
     6961 cgtcaagctg ccgatcatgc ccaacgccaa aatcgataaa agaattttcc gcaggcggga
     7021 tagccgccat ccttcaaccc cgacgattga ttccgggtta gtcatgaacg gctaccgggt
     7081 tcatcattta tgttagacgg accgattctg ttaatcggcc tactccatcg cagaggtttc
     7141 tcaaaatatc tccattctta gaatttagaa ataatcctgt tgaagttctt tctaatgaga
     7201 acgacctgcc accaatcctg tgcaatttca cggtttcacc gcccagtagt ttggcatcgc
     7261 tgttgcacaa tagctaatgc aagaaacttt taatcggagg ttatctatga accggcacct
     7321 tgttcaaggc atcatcgttg gcgggctatt cgcattgccg atcacctgga tcgccgcttc
     7381 ggacgctctg tccgcaggca gctttccgga gatacttgtc tcggtaatat tcggcttagc
     7441 cgccggcctg tgcatcggtg cattgatcgc cgccaacttc gccatgctcg cgttggaaga
     7501 aaaagaacac gctgtggcgc gacggcgagt cgatgcccat gcccacgcgt aaaccaccgt
     7561 gccgtgaaga tgccacggac gcgaagcgtt ccttcagcgg ggcaagacgc gccgtgccct
     7621 gccaatttcg cgcttgaaga tttgcctggt ctgccgcgca aatcttcggt tgcaacgcag
     7681 caaaggtgcc gaccgaagcg ggcgagtttc gaatcggcgc ccacattcac tgttctttcc
     7741 tcgagctctt tgcgttctat gcatcccgat cgcccctgag cattatcgaa gggtgcggca
     7801 cctgctgaat cctatctaga caattgcatt gaaaatcgcc ttgttcaccg ccgggcgatg
     7861 cgctatggct ttgccatgtt ttcatgagcg aactccaaca tgaaaaagta cttagctaag
     7921 cgtaaaaaaa cctcccgtgg gttacttgtc gcgtcggctt cgcccagcgt cgctgcggcg
     7981 ccgccgccgg tgctcaaaga agccggcagc tttttcgtca atggcgaggt agtccagacc
     8041 gacaatcccg gcggcggcaa tcccggcggg cgcatcgtcg tcaaccagat gtacgtcgag
     8101 tacgccatcc cgcagaagca aaaagccggc gcttggccgg tgatcatggt gcatggctcc
     8161 ggccacaccg gcaaaaccta cgacaccacg cccgacggcc gcgacggctg gaagcagcat
     8221 ttcctgcgca agggatattc cgtttacgtc gtcgatcagg tcggccgcgc ccgttcgggt
     8281 tggaacaccg agccgatcaa caacgccgag accaaaggcg aagccacgtt gatacctgtg
     8341 ggcggtatcg tgcagttcac ctatgagcgc gcctggtcga tctttcgctt cggccccaag
     8401 cccgatgaat ggtgggccga ttcgaagttt cccaaggatt gtatagacca atacatggcc
     8461 cagctcgtgc ccaataccga agcgaccctg gcgcaaccgc cacgcaagac cgttgacgcc
     8521 ctcatcgcgc tcatggagaa aatcggcccg tcgatcttga tggtccactc ccaatcggga
     8581 gtctacggtg cgctcaccgc cgtcgcgcgc cccgacttgg tcaaaggctt tgtcaacgtc
     8641 gaaggccgaa ccggctgcga tctggacccg caacaaatta aaatcattgc caaggtgccg
     8701 atgctggtag tcgcagggga tcacgagtgg aacggtgaag agcattgccg caaaaccgtc
     8761 gctgccatca acaccgccgg cggcaaatcg acgttcatgg gcacctacga aagggacgtg
     8821 cgcggcaaca cccacatgat gatgatggat tcaaataatc tgcaggtcgc ggaatggatt
     8881 ttggcatggg tggagaagaa cgttccgaag aaatcgcaag tgagtaaaag ataaagccgc
     8941 attgccagac aaccactgaa gtatgcggct ttgaatattc accggatcat cgtgccaatt
     9001 cacccttctc ggtgaattcg cgcgaaaaac cccgcgatag agtgtcattt aacacgacgc
     9061 tatgggtgcc tgcacaacgc ctacgacaag ttatgtcttg gcgattgccg atttaatctc
     9121 gtccaggcga cgctgcgctt cttgctcggt catcgcctcc acctcgcgca gcatttgcgg
     9181 cagctccgct tggcttgcac gcttcgcctg attttgcctg atgactgccg ccatcatggc
     9241 aaccgtaggc gactgaaata aagaatgaag cggcagctct aattggaatt gtttgatgac
     9301 ctgtgagatc acttgtgtcg cgctaaggga gtggccgccg agatcgaaga agttatcgtg
     9361 gatgcctacg ggacgcacat cgaggacttt tccccaaatc gacgccagag tcttttcaat
     9421 ctcatctgtc ggcaaagcat aggctgtgct cagctctggt cgcttatcac tcggttcagg
     9481 caaggcccta cgatcgagct tgccgttggt gaccggtagc gagtccaaaa acataaaagt
     9541 cgacggcacc atgtagcccg gaagcttatc tctgagaaac atccgaagtt gatcgatcgt
     9601 tgcgtgattc gggccacgcg agacgatata ggctgtcaaa tacttatcgc cgttgtcccg
     9661 ctcccatgcg gtaaccccgg catccttaac tgcggggtgc tgagtcaatg tcatctcgat
     9721 ctctatctcc acgcgatacc cacgtatttt gaccatcaaa tccttgcgac ccaaatggac
     9781 aagaagtcca tcaggtaaaa tctgacccaa atcaccggtc agatacgtgc gctcttggcc
     9841 gccgtgtgga tcgggtagaa actttgcccg ggttaaggcc ggatttttcc agtcaaagag
     9901 cctaacaatg cttgccgtcc tctaccggca gcggtccgtg gtggagattg gcactcaatc
     9961 acgtacctgg gctgcggcga tgcctgctca accgagaact g
//
LOCUS       DASQKS010000473         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2372031, whole genome shotgun sequence.
ACCESSION   DASQKS010000473 DASQKS010000000
VERSION     DASQKS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372031"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VE170_12225"
     CDS             complement(<1..1078)
                     /locus_tag="VE170_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16251.1"
                     /translation="MSKAARKQNRKNASSERTALRQGQGARKPRVLFAVGDADAGYSE
                     GRIWQLVERLHKQSGCDVVALTHEAELADAAKKHHIDHQLVPIRPTGVTVTDRLQATD
                     EMIRETANMNIPGSQLPLWKVLAMDDFLSSLQLFGARPTATLDADALIVPLMAVDNNT
                     KGTCGLYTWAVSEARRQGIPVIGLEVSPLGNKNTLSHLPADHFAVKSRWSRDFLIQQG
                     IARSDQVSLLRWEESYLLWPGKDEFTDAYLALEPKAREMLGLAPDRFMILIPHHVAFL
                     WEVRKILDALSRLEFPFTVVIRVDGRTVRRHYHEREIVQESYIHELANLPGVIIDERV
                     GIGLLVQMADLVIAPFAGTATERAA"
     gene            complement(1111..1929)
                     /locus_tag="VE170_12230"
     CDS             complement(1111..1929)
                     /locus_tag="VE170_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HYJ16252.1"
                     /translation="MSNRERVVAIVQARMGSTRLPGKVLANIEGEPMLWHVIERARRA
                     RGVDAIVVATSTAPLDDAIDKFCQSHDIELFRGSESDVLDRYLRAATHYLADNVVRIT
                     ADCPLIDPQIIDRVVAEFLAEDCDYAANVIERTFPHGLDTEVFKYAALVKAAREATAP
                     TEREHVTPYLRDSGRFSWHNVVADVALAPGYYRWTVDEACDLDFVRAVYRCLGTTQFF
                     DWRTVVELLDAHPELTELNAMIVNDAGYIGESGRGPRIPSGRQNGNSNYPPSQR"
     gene            complement(1939..3063)
                     /gene="pseI"
                     /locus_tag="VE170_12235"
     CDS             complement(1939..3063)
                     /gene="pseI"
                     /locus_tag="VE170_12235"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HYJ16253.1"
                     /translation="MSAYIEIAGRRIGPGSPVYVIAELSANHGQDYAQALELVYAAKA
                     CGADAVKLQTYTADTLTLDCDNEWFRIHGTLWHGRKLYELYQEAFTPWEWQPKLKRAA
                     DDIGIALFSTPFDLSAVEFLEAMEVPAHKIASFENGDIALLQQVAKTGKPVIASTGMA
                     SLAEIDELVQTLRVAGACQVALLKCTSAYPAPPEDMNLRTIAHLADAFGVPVGLSDHT
                     LGTTVAVAAVSLGACIIEKHFTLSRAHAGPDSAFSLEPREFKGMVDAIRITEKALGRI
                     HYGLSAAEAKSRSFRRSLFVVKDIKAGERFSDENLRSIRPGHGLHTRYLPHVIGQTAG
                     RDLARGTPLAWNMIGAPVADCLTQTNVSDPARAMIAARES"
     gene            complement(3047..>4363)
                     /gene="pseG"
                     /locus_tag="VE170_12240"
     CDS             complement(3047..>4363)
                     /gene="pseG"
                     /locus_tag="VE170_12240"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HYJ16254.1"
                     /translation="AALPWVILDGYHFTPAYQKAIRHSGCRLVVIDDAAHLARYDADI
                     VLNHGALAPRLEYQVNTDAWLLLGTRYALLRGEFERWRDFQRPIPETARNILLTLGGA
                     DGDNLTAKVVDALALIKDCAFDVQILAGPLNPHIDALRRHAAATPNMQIHTDVTDPSP
                     LMAWADIAIAAAGTTAWELAFMQTPALLLVVAENQTAVAAGVTEFAAGRSLGRAETLS
                     LDAIAAALRELINDPARRRLMAERGKNLVDGGGPARVLAAMHERQSFSCQSEFTIRRA
                     GVDDALLLWQWANDPLTRSNSLSNAAISWDDHQAWCAKKFAAPDCRLWIMQIGALPVA
                     QIRYDRVVSNVGVAAEISFSVAPGFRGRQLGTRLLEATVPLAVDELGADSIQAVALQG
                     NEASRRVFLKAGFAVAEAHRPDGRRCMVFRHDYAANSAPRSHVSLH"
BASE COUNT          884 a         1314 c         1278 g          887 t
ORIGIN      
        1 gggccgcgcg ctcggtggcg gtgccggcga atggcgcaat gacgagatcg gccatctgca
       61 ccagtagccc gataccgact cgctcgtcga tgatcacccc cggcagattg gccaactcgt
      121 gaatatagga ttcctggacg atttcgcgct cgtggtaatg gcggcgcact gtgcgtccat
      181 cgacgcggat caccaccgta aaggggaatt ccaaccggct gagggcatcg agaattttgc
      241 gcacttccca gaggaacgcc acatgatgtg gtattagaat catgaaccgg tcgggcgcca
      301 gaccgagcat ctcgcgggcc ttgggttcga gcgcgagata ggcgtcggtg aattcatctt
      361 taccgggcca caagagataa ctctcttccc accgtaataa tgaaacctgg tcgctgcgag
      421 cgattccttg ttggattaag aagtcgcggg accagcggct cttaaccgcg aaatgatccg
      481 ccggcaggtg actcaaggta tttttattgc ccaacgggct cacttccagt ccgatcacag
      541 ggatcccctg gcggcgcgct tcggacactg cccaggtata gagcccacag gtgcctttag
      601 tattattgtc gaccgccatc agcggcacga tcagcgcatc ggcatccaaa gttgccgtcg
      661 gccgcgcacc gaacagttgc aacgaactca agaaatcgtc cattgccaac actttccaga
      721 gcggcaactg gctgccgggg atattcatgt tggcggtctc gcggatcatc tcgtcagtgg
      781 cctgtaagcg atccgtcacg gtgactccgg tgggacgaat cggcactagt tgatggtcta
      841 tatgatgctt tttagccgca tcggccaatt ccgcttcatg ggtgagagcg acaacgtcgc
      901 agccagactg tttgtgcaag cgctcgacca attgccaaat ccgtccctcg ctatagccgg
      961 catcggcgtc gcccacggca aagagcaccc gtggttttcg tgccccttgc ccttggcgca
     1021 gcgccgtgcg ctccgagctc gcgtttttgc ggttctgttt tctcgctgct ttcgacatgg
     1081 aagtctcctt gaaaatgctc gtgaacgtcg tcaccgttga ctaggtggat aattgctatt
     1141 tccgttttgc cggcccgatg ggatccgcgg cccgcgccct gactcaccga tatatcctgc
     1201 gtcgttaacg atcatggcat tgagttccgt caattccgga tgggcatcga gcaattcaac
     1261 caccgtgcgc cagtcgaaga actgcgtagt gccgagacag cggtacacgg cgcggacaaa
     1321 atccaggtcg caggcttcgt ccaccgtcca gcgataatag cccggcgcca gcgcgacatc
     1381 ggcgacaacg ttgtgccatg agaaccgccc gctgtcgcgc agatagggag tcacatgctc
     1441 gcgctcggtc ggcgccgtag cctcgcgcgc agccttgacc agcgcggcat acttgaagac
     1501 ttcggtatcg agaccatggg gaaaggtgcg ctcgatgaca ttagcggcat agtcacaatc
     1561 ctcggcgagg aactcggcga cgactcggtc gatgatttgc ggatcgatca gcggacagtc
     1621 cgccgtgatg cgcacgacat tgtcagcgag atagtgggta gccgcgcgta agtagcgatc
     1681 gagcacatcg ctctcactgc cccgaaacag ctcgatatcg tggctttgac aaaacttatc
     1741 gatggcgtcg tccagcggtg ccgtcgaggt cgcgaccacg atcgcatcca cgccgcgcgc
     1801 gcgtcgcgcc cgttcgatca catgccagag catcggctcg ccttcgatgt tggccagcac
     1861 tttgcccggt aatcgggtcg atcccatgcg cgcttgaacg atggccacta cacgctcacg
     1921 attggacaag gcgtactcct agctttcccg cgcagcgatc atcgcccgtg ccggatctga
     1981 cacattggtt tgagttaaac aatcagccac cggcgcgccg atcatgttcc aggccaatgg
     2041 cgtgccgcgg gctaaatcgc ggcctgccgt ttgacctatg acgtggggca aatatctcgt
     2101 gtgcaggcca tgaccgggac gaatcgagcg caggttttca tcgctaaagc gctcccctgc
     2161 cttaatgtcc ttgacgacga acaacgaacg gcgaaaggaa cggctttttg cttcggcggc
     2221 gctcaagccg tagtgaatcc gcccaagggc tttttcagtg attcggatgg cgtcgaccat
     2281 gcctttgaat tcccggggtt cgagcgaaaa cgcgctgtcc ggtccggcat gtgcgcgtga
     2341 gagcgtaaaa tgtttttcga tgatgcaggc gcccaggctt accgccgcca ccgccaccgt
     2401 tgtaccgagc gtatggtccg ataaacccac cggcacgccg aaggcatcgg cgagatgggc
     2461 gatggttctc agattcatat cttcgggcgg cgcgggatag gcgctggtgc atttcaacag
     2521 cgccacttgg catgcgccgg ccacgcgcag ggtttgtacc agttcgtcaa tttccgccag
     2581 actcgccata ccggtggacg cgattaccgg cttgccggtt ttcgcaacct gttggagcaa
     2641 tgcgatatcg ccgttttcga aggacgcgat cttatgcgcc ggcacctcca tggcctcgag
     2701 aaactcgacg gcgctcaagt cgaagggggt ggaaaatagg gcgataccga tgtcgtcggc
     2761 agctcgctta agcttgggct gccattccca tggggtgaac gcttcttggt acaactcgta
     2821 gagttttcgg ccatgccaca gagtgccgtg aatgcggaac cactcgttgt cacaatcgag
     2881 cgtcagcgta tccgccgtat aggtttgcag tttcaccgcg tcggcgccgc aagctttggc
     2941 ggcatagacg agttccagtg cttgagcgta atcttgtccg tggttcgccg acagttcggc
     3001 gatcacatag acaggcgatc ccggcccaat gcgccggccg gcgatttcaa tgtaggctga
     3061 catgactccg tggtgcggaa ttggcagcgt aatcatggcg aaacaccatg caccgccgac
     3121 catcggggcg gtgcgcttcc gccacggcaa aacccgcctt aagaaatacc cggcgcgacg
     3181 cttcattgcc ctggagcgcc acagcttgaa tgctatctgc cccgagttca tcaactgcga
     3241 gcggcaccgt cgcttcgagc agacgtgtgc caagttgccg gccgcgaaaa cccggcgcga
     3301 cgctgaagct gatttccgcg gcgacgccaa cattcgaaac cacgcggtca tagcggatct
     3361 gcgccaccgg caaggcaccg atctgcatga tccacaaccg acagtcgggc gcggcaaatt
     3421 tcttggcgca ccaggcttgg tggtcatccc agctaattgc agcattagac agagagttgc
     3481 ttcgagtaag cggatcattg gcccactgcc acagcaacaa cgcgtcgtca acaccggcgc
     3541 gccgaatcgt gaactcgctc tggcagctaa agctttgccg ttcgtgcatc gccgccagca
     3601 cccttgccgg accgccgcca tcgactaaat ttttgccgcg ctcggccatc aaccggcggc
     3661 gcgctgggtc gttgatcaac tcgcgcaggg cggcggctat ggcgtcaagg cttagtgttt
     3721 cggcgcgccc tagcgatcga ccggcagcga actcagtgac accggcggcg acggcggttt
     3781 ggttttccgc caccaccaac agcagcgccg gggtttgcat aaaagccaac tcccaagccg
     3841 tagtgcccgc cgcggcaatg gcaatatccg cccaggccat tagcggcgac ggatcggtaa
     3901 catccgtgtg tatctgcatg ttcggtgtcg cggcggcgtg gcggcgcagt gcgtcgatat
     3961 ggggattgag cggaccggcg agaatctgca cgtcgaatgc gcaatctttt atcaacgcca
     4021 aggcgtccac cactttcgcc gttagattat cgccatccgc accgcctaaa gtcagcaaaa
     4081 tatttctcgc ggtttccgga atagggcgct ggaaatcgcg ccagcgctcg aactccccgc
     4141 gtaatagcgc gtaacgagtc ccgaggagta gccaggcgtc ggtgttgact tgatactcca
     4201 ggcgcggcgc gagagcgcca tgattgagca caatatcggc gtcgtagcgc gctaaatggg
     4261 cggcgtcgtc aattactacg agccggcaac cgctgtgccg gatggccttc tgataggccg
     4321 gcgtgaaatg gtagccatcc aaaattaccc aaggcagcgc agc
//
LOCUS       DASQKS010000474         8548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3199324, whole genome shotgun sequence.
ACCESSION   DASQKS010000474 DASQKS010000000
VERSION     DASQKS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199324"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VE170_12245"
     CDS             <1..383
                     /locus_tag="VE170_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16255.1"
                     /translation="DVGAMVEMPGGGLTTLAKTIQDRPVDTKRVIRSLQMAKEEIRKS
                     KPKSLDLIVKLLCMDREAASETYDHFLTTLSPTGVPTRVGMDILVKSVQSQGRHVDRK
                     VAFTDIADDRLATEVAREMGYKIP"
     gene            542..1513
                     /locus_tag="VE170_12250"
     CDS             542..1513
                     /locus_tag="VE170_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16256.1"
                     /translation="MVLLALFLTLPLPGRAQQPKKVSRIGYLSSNDAATESTRAEAIR
                     RALHERGYVEGQNIATEYRYSEGKFDRVPDLAAELVRLKVDIILVGGAIPIRAAKNAT
                     KTIPIVMTGIGLDPVESGLVDSLARPGGNVTGLTLLSRELGGKRLELLKEAIPKLTRV
                     AVLYDPDLPSTRLEVKEDLPVAARALRLTLQPWEVRDASDFDKVFTAIGQQRPDGLYV
                     PGAGSLMNANRKRIVGFVLKSRLPSVHSNSEAVDAGGLMYYGADQTESYRRVAYYVDR
                     ILKGAKPADLPVEQPTKFELVINLQTAKQIGVTVAPEVLARANKLIR"
     gene            1671..1985
                     /locus_tag="VE170_12255"
     CDS             1671..1985
                     /locus_tag="VE170_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYJ16257.1"
                     /translation="MKTNSTKVKLPKHKIADFCRRHHIRMLAVFGSALREDFRPDSDL
                     DVLVEFDPEHTPGLAFFGMEQELSEMLGRKVDLNTPQFLSPYFRDQVLAEAEVQYAAP
                     RH"
     gene            1966..2310
                     /locus_tag="VE170_12260"
     CDS             1966..2310
                     /locus_tag="VE170_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HYJ16258.1"
                     /translation="MPRHDIDVRLHHMLDAAREAAQMAQGKARADLDTDRPLNLSLVR
                     LLEIVGEAASRISPDERAQYPGIPWMQIVGLRNRLIHGYDNVDFDILWEIVSHDLPPL
                     IAELEKIFSSTP"
     gene            2447..3448
                     /locus_tag="VE170_12265"
     CDS             2447..3448
                     /locus_tag="VE170_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16259.1"
                     /translation="MNRLSPRKLFWKPWVALILVILNAPVASSQEKRAKVRISNAGLT
                     ITALPLLAARDWGTFNANGLDVEIIVMSPPIGAAAMAQGDIDYVAGVGPASVAATLTG
                     LTSRAIWFSSDRISYWIQAAPQYKTLQDLKGKKIALSGGVGGTNHVALTIAMERSGFN
                     PKDYTMVAIPGQQLQIFYSLESGFVEAALMSPPHIFAAAKKGFNKLMDVGAMVEMPGG
                     GLTAMVKTLQERPAETRRVIRALQVAKDEVRKSKPKTVELIMRLLKMDSQGAADTYDA
                     FQTTLNPSGIPNRAGIDNLVRSLQAQGRFTDRKVNFAEVADDRPATEVAKELGYKLP"
     gene            3729..5123
                     /locus_tag="VE170_12270"
     CDS             3729..5123
                     /locus_tag="VE170_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ16260.1"
                     /translation="MDKIDTDIVIVGAGLAGFTAAVRGSEQGAKVLLIDKSRGELGDG
                     NVLMASGSLRAGGKSPKTGAQELYDFVMAEGVGYPDLVNSWSQTCGRAIDWLETVGVA
                     IEETAPGRIWFNQKSEISFGPVYKKDVGPRAIASLKQRFLSLGGSYLNGIEGISLIVE
                     KNRVVGLLGKRDGEPVELRAGAIILTTGGFAANKVLVKQHIGAHADRCKLRGSKQDTG
                     DGLRMALEAGAKAVNLKYFYGHLIARKALSDDRFWPYPRLDSFVDEGLLVDQSGRRFV
                     DEGRGDVAVANELSRTDDPTGATLIFDGESWAASKSSPSSTSLAIPAPNPWILDNGGE
                     LFCDDTAAGLAKKLGVDAANLSDTIEGYNRAVDFKDPGSLAVRRTGKPKVLRAPLYGL
                     RVVSGITFTMGGIAIDGKGRALNHSDQPIAGLYAAGDAIGGLMGGHRGGYTGGLMQAI
                     VTGILAGEDASMRE"
     gene            5149..6132
                     /locus_tag="VE170_12275"
     CDS             5149..6132
                     /locus_tag="VE170_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16261.1"
                     /translation="MTLARGLVLLTLCINLGFGIESTQAQESTRKIRISNSALSVTSL
                     PLLAAREWRLFQERGLDVEIILMSPALTVPAMISGDIDYFAGVGPGVVSASLGGLPFR
                     AVWVSSDRVSYSLVAHPKFKTLQDLKGKKIGVTGSLGATNHVSLVIALEKLAMSPKDF
                     NILALPPQEMLRSLESGFLDAASLNPPTLFMAQKKGFPNILDIGALVEMPGGGLTVLA
                     KDIKAKPEEVKRVIRAMQSAKEIMRKSKEKSVELMTRILKMDQETAMNTYEVFLKTLS
                     PDGIPSRAGMENLVKSIQAQGRFVDKKPAFSDLADDRLAREVAKELGYKVQ"
     gene            complement(6237..6560)
                     /locus_tag="VE170_12280"
     CDS             complement(6237..6560)
                     /locus_tag="VE170_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16262.1"
                     /translation="MGKMKKLVLGGLIAGTLITSAGPVLADSPWWGRPNLARSSRSEL
                     RSDYARLEAARARLEYDRRHNASRYRLAQDRAAIQAILNEIERDRRQAWNTPRGWDRY
                     DRDYR"
     gene            complement(7241..8230)
                     /locus_tag="VE170_12285"
     CDS             complement(7241..8230)
                     /locus_tag="VE170_12285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ16263.1"
                     /translation="MDLPELSQWMKERNLKGHWEHSEWSQTVKPCLWNGEEIMQALNW
                     SGELITTGEAGRRTIQMRNPGLSAGMTNTVHISVQLVKPGEIAAAHRHTAAAIRFVVK
                     GTPNAYTVVEGERFPMFEGDFITTPNWTWHDHFNNSTEPVMWLDGLDVRLVTHFGAMI
                     QENFKKEQQPLEKPDNMSSRVFSHTRPSWVKNDFQAPPYRYPWEETNAALQALKESAG
                     DPYDGVLLEYTNPVDGGHTLPTCSCSIQLLRPKEKTKSHRHTSTTVYYAFRGQGTAKI
                     SANEFSWRQGDIFVVPSWMWHSHENTTSDDAILFSISDRPSTEALGLYRHEGE"
     gene            8340..>8548
                     /locus_tag="VE170_12290"
     CDS             8340..>8548
                     /locus_tag="VE170_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16264.1"
                     /translation="MKIVETALVMTFGLPRPGIAHVLRTICFIAVQLCSGCLIDQIGG
                     AFTQPPERLEQHASDATKKLIDQAF"
BASE COUNT         1875 a         2466 c         2429 g         1778 t
ORIGIN      
        1 tggacgtcgg cgccatggtc gagatgcccg gcggcggtct caccactttg gctaagacca
       61 ttcaagaccg gccggtggac accaaacggg taatccgctc gctgcaaatg gccaaggaag
      121 aaattcgcaa gtcaaagcca aaaagcttgg acttgatcgt caagcttttg tgtatggacc
      181 gggaagccgc cagtgaaacc tacgatcact tcctcaccac cctcagtccg accggcgttc
      241 ccacacgcgt cggcatggat atcttggtca agtcggtgca gtcccaaggc cgccacgtcg
      301 accgcaaggt cgcgttcacc gacatcgccg acgaccgctt ggccaccgaa gtcgctagag
      361 agatgggcta caaaattcct tgagcagtgg cgagcaagaa ctctgccctt ccctactgtg
      421 cggaagctag tcgcgcgacc tatcttgttg ctggacacga agccatgttc agaaaatacc
      481 cggacccggg caagctttgc ttagaatgcg gctaaggtcc gtgcacaaaa aattcactac
      541 attggttcta ctcgcgctgt tcttgacgct tcccttgccg ggtcgggcgc agcagccgaa
      601 gaaggtatct cggataggct atctatcgtc gaacgacgct gctactgagt ccacccgcgc
      661 cgaggcaatt cggcgggctc tgcacgagcg tggctacgta gaaggacaga acatcgctac
      721 cgagtaccga tattcggagg gaaagttcga ccgagttcct gaccttgcgg ccgaattggt
      781 ccgactcaag gttgatatca tcttggtagg aggagccata ccgatccgag ccgccaagaa
      841 tgcgaccaaa acgattccca tcgttatgac gggcattggg ctcgatcctg tcgagtcggg
      901 ccttgttgac agccttgccc gtcccggcgg taacgtcacc ggccttacac tcctttccag
      961 agaactaggc ggtaagcggc tggagctgct caaggaagcc attcctaaac tgacccgcgt
     1021 cgcggttctc tacgacccag acctgccgtc cactagactc gaggtgaaag aggatcttcc
     1081 ggtagcggcg cgcgcattac ggttgaccct tcagccttgg gaggtgcgag acgcgagcga
     1141 tttcgataaa gtttttactg ccataggcca acagcgcccg gacggactct atgtgccggg
     1201 cgcgggttcg ctaatgaacg ctaacagaaa acggattgtc ggctttgtgt tgaagagccg
     1261 gttgccgtcc gtgcacagca actcagaagc cgtagatgcc ggcggactca tgtactacgg
     1321 ggcggaccaa acggagagct accggcgcgt cgcttattac gtcgatagaa ttctgaaggg
     1381 cgccaagccc gccgacctgc cggttgagca accgaccaag tttgagcttg tgatcaacct
     1441 ccaaacggcg aagcaaatcg gcgtgacggt tgcgccggag gtgctggcgc gcgcaaacaa
     1501 gttgatcaga tgatccaatc ggtgatttga gtctgcgggg ctgcatgcgg tctgcgggcc
     1561 gctttggatt ctgctaatga ccgtctcccg atggattgcg atgagcgaat cggttgagct
     1621 gaaccgcgag aagagagtat gctttttgtg agggacgagg tgcgcgaacg gtgaagacca
     1681 attccaccaa ggttaaactt ccgaagcaca agatcgccga cttttgccgt cgtcatcaca
     1741 tccgcatgtt ggcggtcttt ggctcggcgt tgcgcgaaga ttttcgccca gacagcgacc
     1801 tggacgtgct ggtggagttc gacccggagc acacacctgg gctggcattt ttcggcatgg
     1861 agcaggagct ttccgaaatg ctcggacgaa aagtcgactt gaacacgccg cagtttctaa
     1921 gtccctactt ccgcgaccaa gtgctcgcag aggcggaggt gcagtatgcc gcgccacgac
     1981 attgacgtgc ggttgcatca catgctcgac gctgcgcgcg aagcggcgca aatggcgcag
     2041 ggcaaggctc gcgcggattt ggatactgac cgtccgctca acctttcttt ggtgcggttg
     2101 ctcgagatcg tcggcgaagc cgcgagccgc atttcgccgg atgagcgcgc acaataccct
     2161 ggcattccgt ggatgcagat tgtcggcttg cgcaatcgac tcatccacgg ctacgacaat
     2221 gtcgacttcg acattctctg ggaaatcgtc tcccacgatc tgccaccgct gattgctgaa
     2281 ttggagaaga ttttttcttc gacgccgtaa gaatttcaat agcgagtagc acttgtgaaa
     2341 gcggccacgc agatcgggcg tgacaattgc gcctgaagta ctcgcgcgcg cgaacaagtg
     2401 attcgatgac agggacagaa gttaaaaaac cagttagaga acaaccatga atcgattatc
     2461 accgcgaaaa ttattctgga aaccatgggt cgcactgatc ctggttattc tcaacgcccc
     2521 cgtggcatcg agccaggaaa agcgcgccaa ggtacgaatc tccaacgccg gcctgaccat
     2581 caccgcgctg ccgctattgg cagcgcgcga ctggggcaca ttcaacgcca atggtctcga
     2641 cgttgaaatc atcgtcatga gcccgcccat cggcgccgcg gcgatggctc agggggatat
     2701 agattacgtc gccggcgtcg ggccggcatc agtggccgcg accctcaccg gattaacgtc
     2761 gcgagcgatt tggttttctt cggatcggat ctcctactgg atccaagccg cgccgcagta
     2821 caagacgctg caggatctca aaggcaaaaa gatcgccctt tccggcggtg tcggcggcac
     2881 caaccatgta gccctgacga ttgccatgga gaggtccggc ttcaacccga aagactacac
     2941 gatggtcgcc atccccggcc aacaactgca gattttctac tccctcgaat ccggtttcgt
     3001 cgaggccgcg ctgatgtcgc cgccgcatat ttttgccgcg gcaaaaaaag gctttaacaa
     3061 actcatggac gtcggcgcca tggtcgagat gcccggcggc gggctgaccg cgatggtgaa
     3121 aaccttacag gagcgtcctg ccgaaacacg acgggtcatc cgcgcgctgc aagtggccaa
     3181 agacgaggtg cgcaaatcca aacccaagac cgtcgaattg atcatgcggc tgttgaagat
     3241 ggacagtcag ggcgccgccg acacctacga tgcgttccag acgacgctga atccgagcgg
     3301 cattcccaat cgcgccggta tcgacaatct ggtgcgctcg cttcaagccc agggccgctt
     3361 caccgaccgc aaagtaaact tcgccgaagt cgccgacgat cggccggcca cggaagtggc
     3421 caaggaattg ggttacaaac tgccgtagat gtgatttaag aattggccgc aaagaacgca
     3481 aaggaacgca taataaatct taatgtaggg gcgggtttga aacccgccct ctgcggtttg
     3541 taacgatgga gcgttgtact ggagggtttg cgagagctgc gcaatctatc aacgttagta
     3601 gcacgaaatt cacgaagatt ccggatgacg aaggtagggg cgaaaaagcg gccccgctga
     3661 ctttcgcccc tcaatgaacc gccgtgtcct acgtggtgaa tcatgatttc tgctgataag
     3721 agatagccat ggataaaatt gacacagata ttgtcatcgt cggcgccggc ttagcgggct
     3781 tcaccgccgc ggtgcgcggg tcggagcaag gcgccaaagt tttactcatc gacaaaagcc
     3841 gcggcgagct gggcgacggc aacgttttga tggcgtccgg cagcctgcgc gccggcggca
     3901 agagtccaaa gaccggcgcc caggaactct acgacttcgt catggccgaa ggcgtcgggt
     3961 atcccgacct ggtaaactct tggtcgcaaa cctgcggccg ggccatcgac tggttagaga
     4021 cggtcggtgt cgcgatcgaa gaaaccgcgc cgggaagaat ttggttcaat cagaagagcg
     4081 aaatctcctt cgggcctgtc tacaaaaaag atgtcggccc gcgcgccatc gccagtctca
     4141 agcagcgctt tctaagtctc ggcggcagct atctcaacgg catcgaaggc atcagcctga
     4201 tagtggaaaa aaaccgggtc gtcggcttgc taggcaagcg cgatggcgag cccgtcgaac
     4261 tccgtgcagg cgcgattatt cttaccaccg gcggcttcgc cgccaacaaa gtactggtca
     4321 agcaacatat cggcgcccac gccgatcgct gcaaactgcg cggctccaaa caggacaccg
     4381 gcgacggtct gcgcatggcg ctggaagccg gcgccaaggc ggtcaacctg aaatatttct
     4441 acggccactt gatcgcccgc aaagcgctca gcgacgaccg tttctggccc tacccgcgct
     4501 tggactcctt cgtcgatgaa ggactgctgg tcgaccagag cggccggcgc ttcgtcgatg
     4561 aaggccgcgg cgatgtcgcc gtcgccaacg agctttctcg caccgacgat cccaccggcg
     4621 cgacattgat ttttgacggc gagtcttggg ccgcatcgaa gagttcgccg tcttcgacgt
     4681 cgttggctat ccccgcgccc aacccgtgga tactcgacaa cggcggcgaa ttattttgcg
     4741 acgacacggc tgccggctta gccaagaaac tcggcgtcga tgccgccaac ttgagtgaca
     4801 ccatcgaagg gtataatcgc gccgtggact tcaaggaccc cgggtcgttg gcggtaaggc
     4861 gcacgggcaa accgaaagta ttgcgcgcgc cattgtacgg cctgcgggtg gtctccggca
     4921 tcactttcac catgggcggg attgccatcg acggcaaagg ccgagcgctc aatcacagcg
     4981 accaacccat cgccggcctc tacgctgccg gcgacgccat cggcggctta atgggcggcc
     5041 atcgcggcgg ctacaccggc ggcctgatgc aagcgatcgt gactggaata ttagccggcg
     5101 aagacgccag tatgcgcgag taagcgatcg atgggcagca aatactccat gacacttgcc
     5161 cgaggattgg ttctactgac tctatgcatc aatctcggat tcggcatcga atccacccaa
     5221 gctcaggaat ccacacgcaa gatcagaatc tccaactcgg cattgagcgt gacgtcgctg
     5281 cctttgctgg cggcgcgcga gtggcggctg tttcaggaac gcggcctcga tgtggaaatc
     5341 attctcatgt caccggcgct gaccgtgccg gcgatgatct ccggcgacat cgactacttc
     5401 gccggcgtcg gccccggtgt ggtcagcgcc agtctcggcg gcttgccgtt tcgcgccgtc
     5461 tgggtttctt ccgaccgagt ttcttactca ctggtggccc atcctaaatt caagacattg
     5521 caagatttaa aaggcaaaaa gatcggcgtt accggcagcc tgggcgcgac caatcacgtc
     5581 tcgctggtca tcgccttgga gaaacttgcc atgtcgccga aggattttaa tattttagcg
     5641 ttgccgccgc aggagatgct gcgctcgctg gaaagcggct ttctcgacgc cgcctcgctc
     5701 aatccgccga cgctgttcat ggcgcagaag aaaggctttc caaatattct cgacatcggc
     5761 gcgctggtcg agatgccggg cggcggcctc accgtcctag ccaaagacat caaagccaaa
     5821 cccgaagaag ttaagcgggt gattcgcgcc atgcagtcgg cgaaagagat catgcgcaag
     5881 tcgaaagaaa aaagcgtcga gctgatgacg cggattttga aaatggacca ggaaaccgcg
     5941 atgaatacct atgaagtttt tttgaaaact ctaagtcccg acggcatccc gtcgcgtgcc
     6001 ggcatggaga atctcgtcaa atcgatccaa gctcaaggcc gcttcgtcga caagaaacca
     6061 gccttcagcg acctcgccga cgatcgcctg gcgcgggaag tcgccaaaga attgggttac
     6121 aaagttcagt aattcgtctg ctaagattct gcggcgtcgc cgctgatgag ctaacaagcc
     6181 ttaggccgcc agggtttcaa tctggcggcc taagaggcta tggggttagc actaggttag
     6241 cgatagtcgc gatcgtaacg atcccaaccg cggggtgtat tccaggcctg tcgtcgatca
     6301 cgctcgatct cattcaagat cgcctgaatc gcagcacggt cctgggcgag ccggtatcgg
     6361 ctggcgttat ggcgccggtc atattcgagt ctggccctgg ccgcttctag gcgggcatag
     6421 tccgaacgca gctctgagcg gcttgaacgc gcgagattcg ggcgccccca ccaaggacta
     6481 tcagccagca ccggtccagc cgaggttatc aacgtccctg cgatcagacc gcccaacacg
     6541 agttttttca ttttgcccat tttagtctcc ttttctctta gtttcagcca ccgtctgcct
     6601 caacttcctt acctatttag acgcaacctg ggcaaaaggt ttacatggca ccgacgtaag
     6661 agactttgat aagaacgaga actattatgc ggcccttttc ggatcgagcg tcttttcggc
     6721 ctatcacctc aaagacggcg caaagatttg ggtaatcacg gaagccgatg gatcctcgac
     6781 ctgccttctg ccgcctaatg agtattgaag ccagaccggg caagaattct tttcgggggt
     6841 cttcaattca agtttcccag cgctatgctt aagtcagtag ttggcgactc gtgtaagagt
     6901 tagctctgct atttggcttt gcctccccat agcacagcag gggtggatta cagcttgggt
     6961 gttaccgcaa cgtaccgcca actcaggcgc ctcaatcggg gcgccttttt ttatgctcgg
     7021 gcccctctct ccaacggtat tactggctct tactaatgaa cgctttccgc taactgttcg
     7081 ccgatcatag attaatggat tctgcatctc gttctgcgcg ctggaggcgg cgccccagta
     7141 aaacaaaagg ggcgcagaaa actgcgcccc ttttacattc acaggtttac cggcgttaaa
     7201 tgccaatttg aaaaacgcgc gagtcacgcg cacgcatcaa ctattccccc tcgtgccgat
     7261 acaacccgag cgcttcggtg gacgggcggt cgctgatcga aaacaggatc gcgtcgtcgc
     7321 tcgtggtatt ctcgtgggag tgccacatcc atgacggcac gacgaaaata tcgccctgcc
     7381 tccaggagaa ttcattggcc gagattttag ccgtaccctg gccgcggaag gcgtagtaaa
     7441 ccgtcgtgct ggtgtgacgg tgcgacttgg ttttttcctt ggggcgcagc agttggatcg
     7501 aacaggagca ggtcggcaag gtgtgaccgc cgtccaccgg gttggtgtac tccaggagaa
     7561 cgccgtcata gggatcgccg gcgctctctt tgagcgcttg caaagcggcg ttggtctctt
     7621 cccagggata acggtatggc ggcgcttgaa aatcattttt cacccagctt ggccgggtat
     7681 gagaaaacac cctcgacgac atattatcgg gcttttccag cggctgctgc tcttttttga
     7741 aattctcctg gatcatcgcg ccaaagtggg tcaccagccg gacgtccaaa ccgtcgagcc
     7801 acatcaccgg ctcggtggaa ttgttgaagt ggtcatgcca agtccagttg ggcgtggtga
     7861 tgaaatcgcc ttcgaacatc gggaaacgct cgccttcgac aaccgtataa gcgttcggag
     7921 tgcctttgac gacgaaacgg atcgccgccg cggtgtggcg gtgcgccgca gcgatctcgc
     7981 cgggcttcac cagctgcacc gagatatgca cggtgttggt catgccggcg gagagtcccg
     8041 gattgcgcat ttgaatcgtc cgccgcccgg cctcgccggt ggtgatcaac tcgccggacc
     8101 agttgagcgc ctgcatgatc tcttcgccgt tccacagaca gggcttgacc gtctgcgacc
     8161 attccgagtg ttcccaatgg ccctttaagt tgcgctcctt catccactga ctgagttctg
     8221 gcaaatccat agtttcctcc tgccggtctg acgacgattc aacaattgac tctgatccat
     8281 acaaacggag ccgctgactg tcaagccaag gcgccgattg acagatcgat tgacagcacg
     8341 tgaagattgt ggagactgcc ttggtgatga ccttcggttt gccgcggcct ggaattgctc
     8401 atgtcttgcg gacaatctgt tttatcgccg tccaactatg ttcgggctgc ctgatcgatc
     8461 aaatcggcgg agcctttacc caaccgccgg aacgattaga gcaacacgct tccgacgcaa
     8521 ccaagaaact catcgaccaa gctttcga
//
LOCUS       DASQKS010000475         4488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3201292, whole genome shotgun sequence.
ACCESSION   DASQKS010000475 DASQKS010000000
VERSION     DASQKS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3201292"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VE170_12295"
     CDS             <1..545
                     /locus_tag="VE170_12295"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16265.1"
                     /translation="LTQLMVQKHGLVPNKDVTSLVIGGQEDTVLALRTGRIAAALLTP
                     PRPLMLAREGFNRIGYSGDYMPTYPSGGIGVTDDTIKNKPNELYAFVKGSVRGLQFAR
                     QNRAEGKKILTDYFAIKDPALADQFFELYLSRLPVNGSADEAWMKGAIEFTQKSLGDA
                     AKEAAPHQVFDFSFVNKAAR"
     gene            complement(667..2289)
                     /locus_tag="VE170_12300"
     CDS             complement(667..2289)
                     /locus_tag="VE170_12300"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HYJ16266.1"
                     /translation="MATPEFVYQDPLPLGKDTTQYRLLTKEHVSVTQFEGKDILKVAP
                     EGLTHLANVAIRDVSFLLRTAHLEKVAAILKDPDASQNDRYVALAMLRNADTSSKGIL
                     PFCQDTGTATVFGKKGQQVWTGVKDEEFIAKGIYKTYTEENLRYSQTAPLNMWDEVDT
                     GTNLPAQIDLMATDGMEYNFLFVTKGGGSANKTMLYQETKALLNPASLEKFLTEKMQT
                     LGTAGCPPYHLVFVIGGTSAEACLKTVKLASTGYLDHLPTTGNAHGRAFRDTEFEGRM
                     LQRAYKSGYGAQFGGKYFALDLRVIRLPRHGASCPVGMGVSCSADRNVKAKINKDGIW
                     LEELEKEPGRLIPEEYRGKFVANVVQVDLNRPMQEIVAQLTKYPVTTQLSLNGTMIVA
                     RDIAHAKLKERLDKGEGLPQYLKDHPIYYAGPAKTPAGLPSGSFGPTTAGRMDSYVEL
                     FQANGAALITLAKGNRSQQVTDACKKYGGFYLGSIGGPAAILAKENIKKVELLEYPEL
                     GMEAVYKIEVEDFPAFILVDDKGNDFFKQILH"
     gene            2419..3402
                     /locus_tag="VE170_12305"
     CDS             2419..3402
                     /locus_tag="VE170_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16267.1"
                     /translation="MKFWLASLLASLTLASSAQAQTLDQLIAGAKNEGKLVIYASAAA
                     PQLQMYMAAFGKRYPFIKTEYFRTDKQKLVTRVLLEEQAKQRLADVVHTSVIETHILK
                     KRGALSRYLPMEAASYPSQYKDPEGYWTSVYASGTLMGFNTRQVKRAEAPKSYDDLLN
                     PRWKNAIAVDNTKIEWFSMLLKLKGRGFMEKLAAQNGPVMAGNTNLLNLLAAGEFAIM
                     AGVYEYSVDNLKNKGAPVDWIGLEPVIVYTVAASLPSQPAHPFAAKLFIEWLLTKEGQ
                     EVINQYGRVPIRDDIDSKYGRLLKQHKLLMTDVDLSQREVEVNEMIRKLFR"
     gene            3522..>4488
                     /locus_tag="VE170_12310"
     CDS             3522..>4488
                     /locus_tag="VE170_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYJ16268.1"
                     /translation="MRLVIAAEQLHTYCAMCTSRCGVIATVEDGRLTQINADPDHPNG
                     CICVKGTAAPEIVYSPDRLLRPLLRTRAKGDPNPGWVEITWNQALSLAALRLSDIKDN
                     YGAEAVVFSRATTAGSAAIDFDGWVQRLANAFGSPNLLTSNHICTWNRRVGSKYTYGS
                     GIPLPDLDNTQCMLIWGINPPATSPAQAMRISRARNRGAKLIVIDPRKTTLAVKADSW
                     LRIRPGKDGELAMAMIHVLLEENLFDADFARAWTNGPFLIRSDNHQLLTEGDVRANGR
                     PNTWLVWDKLDGLRAHHETDKPMLFGSHSVTLLDGRKIECRPALEL"
BASE COUNT          944 a         1354 c         1243 g          947 t
ORIGIN      
        1 tgctaacgca attgatggtg caaaagcatg ggttggtgcc gaacaaagat gtcaccagcc
       61 tggtcatcgg cggccaggaa gacaccgtgc tggcgctgcg caccgggcgc atcgccgctg
      121 cattgctgac cccgccgcgc ccgcttatgt tagcgcgcga agggtttaac cgcatcggct
      181 actccggcga ctacatgccg acctacccga gcggcggcat tggcgtcacc gacgacacca
      241 taaagaacaa acccaatgag ctgtacgcct tcgtcaaagg cagcgtccgc ggtttgcagt
      301 tcgcgcgtca gaaccgcgcc gagggcaaga agattcttac cgactacttc gccatcaaag
      361 accctgccct cgccgatcag tttttcgagt tgtacttaag ccgattgccg gtgaacggct
      421 cggccgacga ggcctggatg aagggagcca tcgaatttac ccagaagagc ttgggcgacg
      481 ccgccaagga agccgcgccg catcaggttt tcgattttag ttttgtgaac aaggcggcta
      541 ggtaggcaag ttaagtaggc gggtttgaaa cccgccccta cgaatcccaa ttcctttttg
      601 cgccctttgc gttctttgcg gccattcttc tgtccccttg cggctccgct atgcttgcgt
      661 aactgcttaa tgcaggatct gcttgaaaaa atcgttgccc ttatcgtcga ctaagataaa
      721 cgccgggaag tcctctacct cgatcttgta gaccgcttcc atgccgagct cgggatactc
      781 cagcagctcg acctttttga tgttttcctt ggccaggatc gccgccgggc cgccgatgga
      841 gccgagatag aatcccccat acttcttgca cgcgtcggtg acctgctggc tgcggttgcc
      901 tttggccaag gtgatcagcg cggcgccgtt ggcctggaac aactcgacgt aggaatccat
      961 acgtccggcg gtagttgggc cgaacgatcc cgacggcaaa cccgccggcg tcttcgccgg
     1021 gccggcgtaa tagatcggat gatctttcaa atactgcggc agcccttcgc ctttgtccaa
     1081 gcgttctttc agtttcgcgt gcgcgatatc gcgagcgacg atcatcgtgc cgttcagcga
     1141 cagctgggtg gtgaccggat atttagtcag ctgcgcgacg atttcttgca tcggccggtt
     1201 gagatcgacc tggaccacgt tggcgacaaa cttgccgcga tactcttcgg ggatcaggcg
     1261 acccggctcc ttttccaact cctcgagcca aatcccatcc ttgttgatct tggctttgac
     1321 gttgcggtcg gcggagcacg acacgcccat gcccactgga caggaggcgc catggcgcgg
     1381 caaacgaatc acgcgcaagt cgagggcaaa atatttaccg ccgaactgcg cgccgtaacc
     1441 ggacttgtag gcccgctgca acatgcggcc ttcgaactcg gtatcgcgaa aggcccgtcc
     1501 atgggcgttg ccggtggtcg gcagatggtc gagatatccg gtggacgcca gcttcacggt
     1561 cttcaaacag gcttcggccg aagtgccgcc gatgacgaag accaaatgat acggcgggca
     1621 gccagcggtg cccagggttt gcatcttttc ggtgagaaac ttctccaagc tcgccggatt
     1681 gagcagcgcc ttggtctctt gatacagcat ggtcttgttc gccgagccgc cgcctttagt
     1741 gacgaagaga aaattatatt ccatgccgtc ggtggccatg agatcgattt gcgccggtaa
     1801 gttagtgccg gtgtcgactt catcccacat attgagcggc gcggtttgcg agtaacggag
     1861 attctcttcg gtgtaggttt tgtaaatccc cttggcgatg aactcctcat ccttgacgcc
     1921 ggtccatacc tgctggcctt ttttgccgaa gaccgtggcc gtgccggtgt cctggcaaaa
     1981 cggcaggatg cccttggacg aagtgtcggc gttacgcaac atcgccaggg cgacatagcg
     2041 atcgttttgt gaagcgtcgg gatctttgag aattgccgcg actttttcca gatgcgccgt
     2101 gcgcaagagg aaggacacat cgcggatcgc cacgttagcg agatgggtca acccttccgg
     2161 cgcgactttg agaatatcct tgccctcgaa ctgggtcacc gagacatgct ctttggtgag
     2221 cagacggtat tgggtggtgt ctttacccag cggcagcgga tcttggtaca cgaattcagg
     2281 agtggccatg acgttttcta cctcgtagga tttgtcacaa tcctaaagga aaaaatcgcg
     2341 gttgtcgacg cgcttcaggg ccggggcgtg ttgacactac cgggccgtct catttaccgt
     2401 gccgacatcg gaggaatcat gaaattctgg ctcgcatcat tgctcgcatc cttaacccta
     2461 gcatcgtccg cacaggcaca aaccctcgac caactgattg ctggcgccaa gaatgaaggc
     2521 aaattggtaa tctacgcctc cgccgcggcc ccgcagctgc aaatgtatat ggcggctttc
     2581 ggcaaacgct acccgtttat caagaccgaa tacttccgca ccgacaaaca gaaactggtc
     2641 acccgcgttc tcctcgaaga gcaagccaag cagcgcctgg ccgacgtggt tcacaccagc
     2701 gtgattgaaa cccatatctt gaaaaagcgc ggcgcgctga gccgctacct gccgatggaa
     2761 gcggcctcct acccgagcca atacaaggac cccgaaggtt actggacgtc ggtttatgcc
     2821 agcggcacgc tgatgggctt caatacccgg caggtcaagc gcgccgaagc gccgaagagc
     2881 tacgacgatc tcttgaaccc gcgctggaag aacgccatcg ccgtcgacaa caccaagatc
     2941 gaatggttct ccatgctgct caagctcaag ggccgcggat ttatggaaaa actggcggcg
     3001 caaaacggac cggtgatggc cggtaatacc aatctgctca acctgctcgc cgccggcgag
     3061 ttcgccatca tggccggcgt ctacgagtac tccgtcgata atttgaagaa caaaggcgcg
     3121 ccggtggact ggatcggcct ggagccggtg atcgtctaca ccgtcgccgc ttcgttgcca
     3181 tcgcaacccg cccatccttt cgccgccaag ctgttcatcg aatggctgct caccaaagaa
     3241 ggccaggaag tgatcaatca atacggccgc gtgcccatcc gtgacgacat cgactcgaaa
     3301 tacggcaggc ttttgaagca acacaaactg ctcatgaccg acgtcgatct gagccaacgc
     3361 gaagtcgaag tgaacgagat gatccgcaag ctgttccgat gaatgctttt tgcgcccttt
     3421 gcgttctttg cggccgaact ccccattccg aaccgggtcg gttctgaccg acctactttg
     3481 tgaatttgtg ccctttgtgg tgaattccct gtctttctga attgaggctc gtcatcgccg
     3541 ccgaacaact tcacacttac tgcgccatgt gcacgtcgcg ctgcggcgtc atcgctacgg
     3601 tcgaagatgg ccgtctcacg cagatcaatg ccgatcccga tcatcccaac ggctgtattt
     3661 gcgtcaaagg caccgccgcg ccggagattg tctactcgcc ggaccgtctg cttcgacccc
     3721 tgctgcgcac ccgcgccaaa ggcgacccca accccggctg ggtcgagatc acttggaatc
     3781 aagcgctcag tctcgccgcg ttgcgcctca gtgacatcaa agataactac ggcgccgaag
     3841 ccgtggtgtt ttcgcgcgca acgaccgccg gcagcgccgc catcgacttc gacggctggg
     3901 tacagcgctt ggccaatgct ttcggcagtc cgaatttact cacctcgaat cacatctgca
     3961 cctggaaccg ccgggtcggc tccaaataca cctatggcag cggcataccg ctgcccgacc
     4021 tcgacaacac tcagtgcatg ctcatttggg gcatcaatcc accggcaact tcgccggccc
     4081 aagctatgcg catcagccgc gcgcgcaacc gcggcgccaa gctgatcgtc atcgacccgc
     4141 gcaaaactac gctcgccgtc aaggccgact cctggctgcg cattcgcccc ggtaaagacg
     4201 gcgaactggc gatggcaatg atccatgtac tgttggaaga aaatctgttc gacgctgatt
     4261 tcgcgcgcgc ctggaccaac ggcccattct tgattcgctc cgacaatcat caattgctga
     4321 cggaaggtga cgtgcgcgcc aatggacgcc ccaatacgtg gttggtgtgg gacaagttgg
     4381 acggattgcg agcgcaccac gaaaccgaca aaccgatgct tttcggtagt cattcagtaa
     4441 cattgctgga cggcagaaag atcgaatgcc gaccggcgct ggaactat
//
LOCUS       DASQKS010000476         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4449083, whole genome shotgun sequence.
ACCESSION   DASQKS010000476 DASQKS010000000
VERSION     DASQKS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4449083"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VE170_12315"
     CDS             <1..773
                     /locus_tag="VE170_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYJ16269.1"
                     /translation="AVWSYGESSAWDVHPAQVSIDVGGIRGAIRAKERRINIASSLVG
                     SVNLQNILGAVGVGCALGLTDDEIARGIARLSAVPGRLEKVDNGRGVAVLVDYAHTPD
                     ALEKALHVTRQLTTGKLIVVFGCGGDRDRGKRPLMGEIAARLSDVAVLTSDNPRTEEP
                     TAILADVEAGVRQVGIAKFDATQLTAGTTGSAARRGYYVEVDRRAAIRLALAIAGAGD
                     TVLIAGKGHEDYQILGKTKFHFDDREVAREAIAERANG"
     gene            785..2194
                     /gene="murF"
                     /locus_tag="VE170_12320"
     CDS             785..2194
                     /gene="murF"
                     /locus_tag="VE170_12320"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HYJ16270.1"
                     /translation="MSWTLGEILAATGGKVTSSGKIARYGEIVTDSKQVKGNSIFVAL
                     KGERHDAHDFVGDAVRRGAACVIVHRSLPTARLGPATIVRVGDTLRALGDLAHYRRER
                     LAPMVLAITGSNGKTTTKEMVAAILAQGSLAGQSLRGRVLKTEGNFNNLVGLPLTLLR
                     LTKRHKVAVVELGTNSPGEIQRLAEIADPDLGVITTVAAAHLAGLKNLPGVAREKGAL
                     YRAVRRNGMIAVNVDDPWVRRLGAKFAGKKLTYGTAGQLRAQGLRVRAGRGMAFALRY
                     GGQQARINLNYLGRHNVTNALGAAALTLGAGVSLAAVRRGLAQAKPFTMRMQRESWRG
                     IGIINDSYNANPGSMQAALETLALIGGRGKKIAVLGDMFELGKQSVKEHRQLGRVAAQ
                     ARIDRLYLLGDQAGTVRRGALRAGMKAHQVAVGADHDDLATRLRQQVKRGDWLLIKGS
                     RGMKMEKVLQALKSVTAQE"
     gene            2198..3274
                     /gene="mraY"
                     /locus_tag="VE170_12325"
     CDS             2198..3274
                     /gene="mraY"
                     /locus_tag="VE170_12325"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYJ16271.1"
                     /translation="MLYHLFTSLSTSYSGFNVFRYITFRAAMAALLSLTISFLLGPWL
                     IRVLTERQIGQHIRDDGPASHAVKAGTPTMGGALIILALLFSTVLLADITNRYVLLAL
                     LATVGSGAVGFADDYLKLTGKNSKGLAPRTKLLGQFAVALVVVLALFYQPKFSTEIAF
                     PFIKTLRLNLDIFYIPFAMLVIVGASNAVNLTDGLDGLAIGPVMVAAAAYAVVAYATG
                     HLKFAEYLQIPYVAGVGELTVFCAAMVAAGLGFLWYNAYPAQMFMGDVGSLSLGAALG
                     IVAVLTKNEILLMIIGGVFVMEALSVIFQVASYKIRRKRVFLMAPIHHHYELKGWKEP
                     QIIVRFWILSIIFAVIGLSTLKLR"
     gene            3290..3577
                     /locus_tag="VE170_12330"
     CDS             3290..3577
                     /locus_tag="VE170_12330"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16272.1"
                     /translation="MELKGKKLLVIGLARTGRECARFLVQQGATVWISDLHGQDALSG
                     EMAALSDLSLTYRLGGEEKSWLDGMDYVVPSPGVPMEISCYARRWRETFRC"
     gene            3538..4623
                     /gene="murD"
                     /locus_tag="VE170_12335"
     CDS             3538..4623
                     /gene="murD"
                     /locus_tag="VE170_12335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYJ16273.1"
                     /translation="MLREALARNIPVLSEIELAYRYFAAPLVAITGTNGKSTTTTLIG
                     KMLEADGKKVFLGGNLGVPFISSVTDAWDWGVIEVSSFQLEWVDEFRPRIALLLNLTE
                     DHLDRYGSFADYCRAKERIFAAQQRADMAILSRDDPRVWQLRERISAQVVSFGFGEVS
                     EGVFATPTEVIWRDGAVEERFSLAKAKIQGVHNVENIMAAVAAAQSAGVSRRAIQTTI
                     DEFPGLEHRLELVREKNGVRYYNDSKGTNVGAVVKSLASFTGRVLLLAGGVDKGGDYA
                     PMAPAVKNQVRRLVLFGAAKHTIAAALGGLTTTVLVDDLNAAVRDAAAHAQPGDVVLL
                     SPACSSFDQFSNYAERGKSFKNLVQAL"
     gene            4620..5714
                     /gene="ftsW"
                     /locus_tag="VE170_12340"
     CDS             4620..5714
                     /gene="ftsW"
                     /locus_tag="VE170_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HYJ16274.1"
                     /translation="MRDALAVDKWILLAVIGLVAMGVVMVLSTSYLYSQERYADGTFF
                     FRKQLFAMGAGVVALIVCSLVPSRQYRRFAYPLLALTLVCLVLVLIPGIGASRGGARR
                     WIALAGLAFQPSEMAKLAIVVYLAHSIAKKEPMIGSFAIGVLPHLIVTGLFAGVLLLE
                     PDFGSALILTMLLYSMLFIGGVRVQHLLGTGLLALPLLIYLMTAAEYRMRRLMSFMDP
                     WSDASGSGFHVIQSLIAFGSGQLWGRGLGESRQKLFYLPEAHTDFVFSVIGEELGLLG
                     AFAVLALFGVIILRGLRLTSRIEEPFDQYLAFGLTVLLGFQALIHMGVVMGLMPTKGL
                     VLPFISYGGSAMLINLAEAGILLSLSRRRV"
     gene            5716..>6369
                     /locus_tag="VE170_12345"
     CDS             5716..>6369
                     /locus_tag="VE170_12345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ16275.1"
                     /translation="MRVVFAGGGTGGHLFPGLAVAREFQRRDGRSEILFIGTEQGIEY
                     RVLPKEGFKLETLAVKGLKGRGIRGFLDALYGVPAGLFRSLAILRAFRPDCVIGLGGY
                     ASGPVLLAARLKHIRCAIMEQNLRPGFTNRLLARWVDWVFTAYRESVAYFPGARVQET
                     GNPVRWTEVPQVKRGEKFTLLIFGGSAGARRINYAVVDALKLLGDLKNDIVITHQTGP
                     "
BASE COUNT         1148 a         1808 c         2022 g         1391 t
ORIGIN      
        1 tggcggtatg gagctatggc gagagcagcg catgggacgt ccatccggcg caggtgagca
       61 tcgatgtcgg cggtatccgc ggcgccattc gcgccaagga gcgccggata aacatcgcct
      121 cgtcgctggt aggctcggtg aacttgcaaa atattttggg cgcggtggga gtcggctgcg
      181 cccttggatt gactgacgac gagattgcgc gtggcatagc gcgcttatca gccgtgcccg
      241 ggcggttgga aaaagtcgac aacggacgcg gtgtcgccgt cttggtcgat tacgcccata
      301 cccccgatgc cctcgaaaaa gcgctgcacg tgacgcggca gttgacgaca ggcaagttga
      361 tcgtggtgtt cgggtgcggc ggtgatcgcg accgcggcaa gcgaccgttg atgggcgaaa
      421 ttgccgcgcg gctgagcgat gtcgcggtgc ttacctccga caatccgcgc actgaagagc
      481 caacggcgat actcgccgac gtagaagccg gcgtgcgtca agtcggcatc gccaaattcg
      541 acgcgacgca gttaaccgcc ggcactaccg gctcggcggc acggcgcggc tattacgtcg
      601 aagtcgaccg acgcgcggcg atccgcttgg cgctggcgat tgccggcgcc ggcgataccg
      661 tgctgatcgc cggcaagggc catgaggact atcagattct aggcaagacg aagtttcatt
      721 tcgacgatcg ggaggtggcg cgcgaagcga ttgcagagcg tgccaacggt taagttaacg
      781 aggcatgagt tggactctgg gagaaattct tgcggccacc ggcggcaaag tgaccagctc
      841 cggtaagatc gcgcgctacg gggagattgt gaccgactcc aaacaggtta aaggcaactc
      901 gatcttcgtc gcccttaaag gcgagcgtca tgatgcccat gatttcgtcg gtgatgcggt
      961 gcgccgcggc gccgcctgcg ttattgtcca tcgctccttg ccgacggcta ggctcgggcc
     1021 ggcgaccatc gtgcgagtcg gcgacacgct ccgggcgctt ggtgatctgg ctcactatcg
     1081 gcgtgagcgg ctggcgccga tggtgctcgc catcaccggc tccaacggca agacgacgac
     1141 taaggaaatg gtcgcggcga tcttggcgca aggaagtttg gccggccagt cgttgcgcgg
     1201 ccgggtgctc aagaccgaag gcaatttcaa taatttggtc ggcttaccct tgacgctttt
     1261 acgcttgacc aagaggcaca aagtcgccgt ggtcgaactt ggcaccaact ccccgggcga
     1321 gatccagcgc ttggcggaaa tcgccgatcc ggacctcggc gtcatcacga cagtagccgc
     1381 ggcccatttg gcgggtctaa aaaacttgcc cggcgtggcc cgcgaaaaag gcgcgctcta
     1441 tcgcgctgtg cgccgcaacg gcatgatcgc ggtcaatgtc gacgatccgt gggtgcgccg
     1501 tctgggcgcc aagtttgccg gcaaaaagtt gacctatggc accgccggcc agctgcgcgc
     1561 acaggggttg cgggtgcgcg ccggccgggg catggccttc gcgctgcgct acggcggcca
     1621 acaagcgcgg atcaacttga attatctagg ccgtcataat gtcaccaatg ccctcggcgc
     1681 cgcggccttg acccttggcg caggagtcag cttggcggcg gtgcgtcgcg gcttggcgca
     1741 agccaagccc tttaccatgc gtatgcagag ggaaagctgg cgcggcatcg gcattatcaa
     1801 cgacagctac aacgctaatc ctggctccat gcaagcggcg cttgagactc tggcactgat
     1861 cggcggccgg ggcaaaaaaa tcgccgtgtt gggcgacatg ttcgagctcg gcaagcagag
     1921 cgtcaaagag catcgccaac tgggccgggt cgccgcccaa gcgcggatcg atcgacttta
     1981 tctgctcggc gaccaagccg gcacagtgcg tcgcggcgcg ctgcgcgccg gcatgaaagc
     2041 gcaccaggtt gccgtcggcg ccgatcatga cgatttggcc acgcgcttgc gccaacaggt
     2101 caaacgcggc gattggcttt tgatcaaagg ttcgcgcggc atgaaaatgg aaaaagtttt
     2161 acaagcgctc aagagcgtga cggcccagga gtagcgcgtg ctgtatcact tgttcacctc
     2221 gctttcgacc agctactccg gctttaatgt ctttcgctac atcacctttc gcgcggcgat
     2281 ggcggcattg ttgtccctga cgatttcgtt tctgctcggc ccatggttga tccgtgtgct
     2341 caccgaacgg cagatcggac aacatattcg cgatgacggc ccggcgtccc atgcggtcaa
     2401 ggcgggcacg ccgaccatgg gcggcgcgct gattatcctg gccttgttgt tctcgaccgt
     2461 tctgctggcg gatatcacca atcgttatgt gctgttggcc ttgctcgcca cggtgggcag
     2521 cggggcggtg ggcttcgccg acgattattt gaagctcacg ggcaaaaaca gcaaggggct
     2581 ggcgccgcgg actaagctct tgggccagtt cgcggtcgct ctcgtggtcg tcctggcact
     2641 gttctatcaa ccgaaattca gcaccgagat cgcctttccg ttcattaaga cgctgcggct
     2701 gaatctcgac attttttaca tccccttcgc catgctggtg atcgtcggcg cctccaacgc
     2761 ggtcaatctc accgacggtc tcgacggctt ggcgatcggc ccggtgatgg ttgccgcggc
     2821 ggcttacgcg gtggtggctt atgccaccgg ccatctcaaa tttgccgaat atttgcagat
     2881 cccctatgtc gccggcgtcg gcgagcttac ggtgttttgt gctgcgatgg tggcggcggg
     2941 cttgggtttt ctttggtaca acgcctatcc ggcgcagatg tttatgggcg atgtcggttc
     3001 gctgtccctc ggcgccgccc tgggcatcgt cgcggtgctg accaagaacg aaattcttct
     3061 gatgatcatc ggcggcgtct tcgtcatgga agcgctctcg gtgattttcc aagtggcgtc
     3121 ctacaagatc cgccgcaagc gggttttctt gatggcgccg atccaccatc actatgaact
     3181 caaaggctgg aaagagccgc aaattatcgt gcgtttctgg atcctgtcga ttatttttgc
     3241 agtcatcggt ttgagcacct tgaaactcag ataacggcga ccatgttaaa tggagctcaa
     3301 aggaaaaaag ctattggtca tcggcctggc gcgcaccgga cgagaatgtg cacgctttct
     3361 cgtgcaacag ggggccacgg tgtggattag cgatctgcac ggacaagacg ccttgagcgg
     3421 tgaaatggcc gcgctcagcg atctctcgct aacctatcgc ttgggcggcg aggaaaaaag
     3481 ttggctcgat ggcatggact acgtggtgcc cagcccgggt gtgccgatgg aaatctcttg
     3541 ctacgcgagg cgctggcgcg aaacattccg gtgttgagcg aaatcgagtt ggcctatcgc
     3601 tatttcgccg cgccactggt ggcgatcacc ggtaccaacg gcaaaagcac gacgaccacg
     3661 ttgatcggca agatgctaga ggccgacggc aagaaagttt ttctgggcgg caacttgggt
     3721 gtgccgttta taagctcggt gaccgatgcc tgggactggg gggtcataga ggtcagcagc
     3781 tttcagttag aatgggtcga cgagtttcgc ccccgcatcg cgctcctgct taatcttact
     3841 gaggatcact tggaccgcta cgggagtttc gccgattact gccgggccaa ggagcgcata
     3901 ttcgcggcgc agcagcgcgc cgacatggcg atcctcagcc gcgacgatcc gcgggtatgg
     3961 cagctgcgcg agcggattag cgcgcaggtg gtttccttcg gctttggcga agtctccgaa
     4021 ggagtgttcg cgacaccgac tgaagtcatc tggcgcgacg gtgccgtcga ggaacgcttc
     4081 tctttagcca aggcgaagat ccaaggcgtg cacaacgtag aaaatatcat ggccgccgtc
     4141 gccgcggctc aatccgccgg tgtttcgcgt cgggcgattc aaaccaccat cgacgagttt
     4201 ccaggtctcg aacaccgtct tgaattggtt cgcgaaaaaa atggagtgcg ctactacaac
     4261 gactccaagg gcaccaatgt cggcgcggta gtgaaatccc tggcgagttt tactggccgg
     4321 gtcctcttgc tcgccggcgg cgtcgacaag ggcggcgact atgcgccgat ggcgccggcc
     4381 gttaagaacc aagtgcgccg cttggtgttg ttcggcgcgg ccaaacacac catcgccgcc
     4441 gccctgggcg gactgacgac gactgtcctg gtcgacgatc taaatgctgc tgtgcgcgat
     4501 gccgccgcgc acgcgcagcc gggcgatgtt gtgctgcttt cgccggcctg ttcgagtttc
     4561 gatcaatttt ccaactatgc cgaacgcggc aagagtttca agaatttggt gcaggcgcta
     4621 tgagagatgc tttggccgtc gataaatgga tcctgctggc ggtcatcggc ctggtcgcca
     4681 tgggagtggt catggtgctt agcaccagct atctctattc ccaagagcgc tatgccgatg
     4741 gcacgttctt ttttcgcaaa caattgttcg ccatgggcgc cggcgtggtt gcgttgatcg
     4801 tctgctcgct ggtcccgtcg cggcaatacc gccgctttgc ctatccattg ttggcgttga
     4861 ctctggtttg tttggttctc gtgctgattc ccggtatcgg cgccagtcgc ggcggagcgc
     4921 gccgctggat tgccctcgcg ggactggctt ttcaaccgtc ggagatggcc aaattggcga
     4981 ttgtcgtcta tctcgctcat tccatcgcca agaaggagcc gatgatcggc tccttcgcca
     5041 tcggcgtctt gccgcacctg atcgtgaccg gcttgttcgc cggtgtgctc ctgctcgaac
     5101 ccgactttgg cagcgccttg atcctgacca tgctgctcta ctcgatgttg ttcatcggcg
     5161 gtgtgcgcgt gcaacatctg ctcggcaccg gcctattagc cttacccttg ctgatctatc
     5221 tcatgaccgc ggcggagtac cggatgcgcc gcttgatgag cttcatggac ccttggagtg
     5281 atgcgtcggg cagtggcttt cacgtcattc aatcgttgat cgcgttcggt tccggccagt
     5341 tgtgggggcg cggcttgggt gagagccggc agaaattatt ttacctgccc gaggcccata
     5401 ccgacttcgt tttctcggtg atcggcgagg agttgggact cctcggcgcg tttgcggttt
     5461 tagcgctgtt cggcgtgatt attttgcgcg ggctgcggct gacctcgcgc attgaggagc
     5521 cgttcgatca gtatttggcg tttggcttga cggtgctctt agggttccag gcgttgattc
     5581 atatgggtgt ggtgatgggg ctcatgccga ccaagggact ggtgctgccg tttatcagtt
     5641 atggcggctc agccatgctg atcaatctgg cggaggccgg cattttgctg agcctatcgc
     5701 gccggagggt atagcgtgcg ggtagttttc gccggcggcg gaaccggcgg ccacctgttt
     5761 cctggactgg cggtggcgcg ggagtttcaa cggcgcgatg gcaggagcga aatacttttt
     5821 atcggcaccg aacagggcat tgaatatcgt gtgttaccca aagaaggatt caaattggaa
     5881 acgctcgcgg tcaaaggact aaaggggcgc ggtatccgcg gctttcttga cgcgctttac
     5941 ggcgtgcccg cggggctgtt tcgttctctg gcgatcctgc gcgcctttcg cccggactgc
     6001 gtcattggac tcggcggtta tgcgtccggt ccggtgttgc tggcggccag gctcaaacat
     6061 attcgctgcg ccatcatgga gcagaatctg cggccgggtt tcaccaatcg gttgcttgcc
     6121 cggtgggtcg attgggtctt taccgcctac cgcgagagcg tcgcttattt tcccggcgcc
     6181 cgggtgcagg aaaccggcaa tccggtgcgt tggacggaag tgccgcaggt taagcgcggc
     6241 gagaaattca cgctgctgat tttcggcggc agcgccggcg cgcggcggat caattatgcc
     6301 gtggtcgacg cattgaaatt actcggcgat cttaaaaacg acatcgtcat cacgcaccaa
     6361 accggacca
//
LOCUS       DASQKS010000477         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4041949, whole genome shotgun sequence.
ACCESSION   DASQKS010000477 DASQKS010000000
VERSION     DASQKS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4041949"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..654
                     /locus_tag="VE170_12350"
     CDS             31..654
                     /locus_tag="VE170_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16276.1"
                     /translation="MIGDPSQFEAIGNRLGFDIVEDTPERLRLAWQGARFPAFLCVGI
                     ALLLLFVSVPIVQALYARGFVGPAGSLWYFPLMNLVLIGIAIFLLTQRRTIVVDSSAK
                     SVELCRRSLYRQTKLIASFDEIENITVNIDQVFSGFAIGGSTSAESFPVPALRVHFSG
                     ADAALLDRGSLRRMEVAGKKIAARIGKPLAIAPELAGGAAPTKPKLG"
     gene            complement(861..1529)
                     /locus_tag="VE170_12355"
     CDS             complement(861..1529)
                     /locus_tag="VE170_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16277.1"
                     /translation="MYKPIIFAIIFLATVASCGILPETLQTKHSADLEGRRIRRIAVM
                     PPALSAAAPAANVPFGAAPVARTSEKEAPESLARFVYAALAALSDWQVVSESEVREAV
                     MSEPAGSEEARLKRVGAMVYADAVMMARMQRFRERVGDEWGAKSPASVAFILDLVDVR
                     RGDIVWSARFDETQKSLSESIFSLGDIGQRGVRWLSADQLTQEGVKKAIGQLHDILSR
                     RPAS"
     gene            complement(1619..>3035)
                     /gene="aspS"
                     /locus_tag="VE170_12360"
     CDS             complement(1619..>3035)
                     /gene="aspS"
                     /locus_tag="VE170_12360"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYJ16278.1"
                     /translation="TDANENTRLKYRYLDLRRPQGLAPLVLRYRMTKLIRDYLDGLGF
                     IDVETPVLTKSTPEGARDYLVPSRIYPGKFYALPQSPQLFKQILMVGGVDRYFQIVKC
                     FRDEDLRADRQPEFTQLDMELSFVQPEDVMDVVEGMISLLFKELKGIELKVPFPRLTY
                     GEAMNRFGSDKPDLRFGLELSDFTDALRNCQAKVFTAAIQKGGVVKGMRIPDGGAMSR
                     KDLDDMTPFVATFGAKGVAWTKITAEGWQSPIAKFISAAEQKEVERICQAQVGDVVLF
                     SADSAKVVHDALGNLRLHLGEKLGLIPEKDYALVWVVDFPLMEYDAQEKRYVALHHPF
                     TAPSDEDMHLLDSEPAKARSKAYDLAMNGIEVGGGSIRIHQVELQRKILGLMGIDAEE
                     AQAKFGFFLEALSFGAPPHGGIAFGIDRLAMLLSGAKSLRDVIAFPKSQRAVCMLTEA
                     PSDVAPRQLRELGIRVSVTDK"
BASE COUNT          615 a          909 c          874 g          637 t
ORIGIN      
        1 ttccctgtca ctgcagcata atacctagcg atgatcggcg atcccagtca gttcgaagcc
       61 atcggcaatc gtctgggctt cgatattgtc gaagacactc ccgagcgatt gcgcttggcc
      121 tggcagggcg cgcgtttccc ggcgtttctc tgtgtcggca ttgcgctgct tctgcttttt
      181 gtcagcgtgc cgattgtgca agcgctgtat gcgcgcggct tcgtcggacc ggccggttcc
      241 ctgtggtatt tcccactgat gaatctcgtg ctgatcggca tcgcgatttt tctgctcacc
      301 cagcgccgca ccattgttgt cgatagcagc gcaaaatccg tcgagctttg ccgccggagc
      361 ctgtaccggc agaccaaact gatcgcgtcg ttcgatgaga ttgaaaatat tacagtgaac
      421 atcgaccaag tattcagcgg ctttgccatc ggcggctcga cctcggccga aagttttccc
      481 gtgccggcac tgcgcgttca tttttccggc gcggacgccg ccctgctcga ccgcggcagc
      541 ctgcgccgaa tggaagttgc cgggaaaaag atcgccgcga gaattggcaa gccattagcg
      601 atcgcgccag aactggccgg cggcgctgct cctacgaaac ccaaactagg ctagctgggc
      661 gccgacgggt ttcgcattcc tattccacgc gaatagactg cgttctagtc ttcgttccga
      721 gttagattct aggtgggaaa acgacccaat ccttcgccgc gctcatcagg acgacagcga
      781 gctaaccact gcgagatttg acatcggttg cagatatgtt tctcagtgaa cggcgcggct
      841 ctcgtgactg atgcaccgta tcaagacgcc ggccggcgcg acaggatatc gtgcaattgg
      901 ccgatggctt ttttgacccc ttcctgggtc agttgatcgg cgctcaacca gcgcacgccg
      961 cgctggccga tatcgccgag agagaagata ctttcgctga gcgatttttg ggtttcgtcg
     1021 aaccgcgccg accagacgat atcgccgcgc cggacatcga cgagatcaag gataaatgct
     1081 accgatgccg ggctcttggc gccccattcg tcaccgactc gttcacgaaa acgttgcatc
     1141 cgcgccatca tcactgcatc ggcatatacc atcgcgccga cccgtttgag gcgagcctcc
     1201 tcgctaccgg cgggttcact catgaccgcc tcgcgcactt cgctctcaga aaccacttgc
     1261 cagtcggaca acgccgccag cgcagcataa acgaaacgcg ccaaggattc cggcgcttcc
     1321 ttttccgaag tgcgcgccac cggcgcggcg ccaaagggca cgttcgcggc cggtgcggcc
     1381 gcggatagag cgggcggcat cacggcaatg cggcgaatcc gccggccttc caaatctgcg
     1441 gagtgcttgg tttgcaaggt ctcgggcaaa ataccgcagc tggcaacggt tgcgagaaaa
     1501 ataattgcaa agataatcgg cttgtacatg ggtgctagcg aaaataacag aagcgctgcc
     1561 gcggctcaag aaaaacttgc gccggcgcgg ctgacaacga ttttccttaa agcgctaact
     1621 acttgtccgt aacgctgacc cgtatgccca actcgcgcag ctggcgtggc gccacatcgg
     1681 agggcgcctc ggtgagcata caaacggcgc gctggctttt tgggaaggcg atgacatcgc
     1741 gcaaggattt ggcaccgctc aaaagcatcg ccagacgatc gatgccgaag gcgataccgc
     1801 catggggcgg cgcgccgaag cttaacgctt cgaggaagaa accgaacttc gcttgcgctt
     1861 cctcggcgtc aatacccatg aggccgagaa tttttctttg cagctcgact tgatgaatac
     1921 ggatgctgcc gccgccgact tcgatgccgt tcatggctaa gtcgtaggcc ttggagcgcg
     1981 ccttggccgg ttcgctgtcg agcaaatgca tgtcttcgtc cgacggcgcg gtgaacggat
     2041 gatgcagcgc cacatagcgt ttctcttgag cgtcgtattc catcagtgga aagtcgacga
     2101 cccaaaccag ggcgtagtct ttttccggga tcagcccgag cttctcgccc aggtgaaggc
     2161 gcaggttgcc cagcgcgtca tggaccacct tggcgctgtc cgcggaaaac agcacgacgt
     2221 ctcccacctg tgcttggcaa attcgttcga cctctttttg ttccgctgcg gagataaact
     2281 tggcgatcgg cgactgccag ccctcggcgg taatcttcgt ccaggcaacg cccttagcgc
     2341 cgaaggtcgc gacaaatggc gtcatgtcgt ccaaatcttt gcgcgacatc gcgccgccgt
     2401 cgggaatgcg catgccttta accaccccgc ccttttgaat cgcggcggtg aaaaccttgg
     2461 cctggcagtt gcgcaaagcg tcggtaaaat cgctgagctc caggccaaag cgcaggtccg
     2521 gcttgtccga gccgaaacgg ttcatcgctt ccccataggt gaggcgtgga aaaggcacct
     2581 tgagctcgat tcccttcagt tccttgaaca ggagcgagat cataccctcg accacatcca
     2641 tgacatcttc gggttgaaca aacgacagtt ccatgtcgag ctgggtaaac tccggttggc
     2701 gatcggcgcg caggtcttcg tcgcgaaagc atttgacgat ctgaaaatag cggtcgacgc
     2761 cgccgaccat caagatctgc ttgaacagct gcggcgattg cggcaaagcg taaaatttgc
     2821 cgggatagat gcggctcggc acgagatagt cgcgcgctcc ctcaggcgtg ctcttggtca
     2881 gcaccggcgt ctcgacgtcg atgaagccca agccatcgag ataatcgcgg atcagcttgg
     2941 tcatgcggta acgcaagacc agcggtgcca ggccctgggg ccggcgcaga tctaggtagc
     3001 gatacttgag ccgggtgttt tcgttggcgt cggtt
//
LOCUS       DASQKS010000478         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5716278, whole genome shotgun sequence.
ACCESSION   DASQKS010000478 DASQKS010000000
VERSION     DASQKS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5716278"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..788)
                     /locus_tag="VE170_12365"
     CDS             complement(162..788)
                     /locus_tag="VE170_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYJ16279.1"
                     /translation="MKAETFFTEDEKERLRQSVVAAESKTAGEIVPMIVTSAARYTEI
                     ELLGVFIGLFAGMLVEWFWSDPWGSPYFQLWPVIGAFAGFLIFRFPAVKRMLASKERV
                     GEAVHTLALASFTDQGLHHTRDHTGILILIALLEHRVEVLADRGINEKVSHGTWDEIV
                     QSISAGIKSGRACDAYCKAIERCGDILATHFPRQADDKDELSNRLVTK"
     gene            complement(791..1594)
                     /locus_tag="VE170_12370"
     CDS             complement(791..1594)
                     /locus_tag="VE170_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HYJ16280.1"
                     /translation="MLRPAAKSKELQKTLLCSLLAAAFVLLLFPLAWSLDVPTLRGRV
                     NDYANVLQSSQAQALETQLAQLERDTGHQVAVLTIPTLDGEDIEGFSIRVAESWKIGK
                     KGHDNGAILVVAVKDRRLRLEVGYGLEGVLPDAIAKRITADYIVPHFRQQDYAGGIVA
                     GINAVDKVLRSEPLPEAARQKTQNRGSNRNSPIMIAITLAVLGLMAFANTGARRRNNM
                     WGGRGRHQGPIIFGGPGGWGGGGFGGGGGSDTFSGGGGSFGGGGSSDSW"
     gene            complement(1782..>2683)
                     /locus_tag="VE170_12375"
     CDS             complement(1782..>2683)
                     /locus_tag="VE170_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYJ16281.1"
                     /translation="AIVPWSPLLAFLVLALNAHLVLARDVPPLQARVNDGAGMMSESQ
                     VSELEDRLIRFEDQSHHQIAVLTIGSLNGDSLEDFSMRVAEAWKIGHKGADDGVILIV
                     ARDDRKIRIEVGYGLEGVLPDAIANRIIQEVIVPRFRDRDFAGGIESGTSAIIQAIKG
                     EQGLNEPPTLHKVPRSSLSAIFTLLVIAVLFGSVVGFTQPSLGRAAFMGAAISGVIGV
                     RAISVIGVGVWLLTILTGALAGMLPVHLARRAWGRSWSAGSSKSSGSAGSRSRVGGSG
                     GGFSGGFRGGGGGFGGGGASGGW"
BASE COUNT          581 a          808 c          760 g          534 t
ORIGIN      
        1 tgggcgaaat gtaggcgaag ttgagtctgt cttgggccag cgcgatccgc caatgggttg
       61 ccaccaaaaa aacggcaagc acgatcacca gttttgtccg catagaggat ggtcctttcg
      121 cgattgagtg accggagaat agcggaaacc cagcggcggc gctacttggt gacaagacga
      181 ttggatagct cgtctttgtc gtcggcttgg cgcgggaaat gggtcgcgag gatgtcgccg
      241 cagcgctcga tcgctttaca gtaagcatcg caggcgcggc cggatttgat accggcgctg
      301 atgctctgga cgatttcgtc ccaagtgccg tggctgactt tctcgttgat gccgcggtcc
      361 gcaagcactt caacccggtg ctccaagagc gcgatgagga tcaggattcc ggtgtgatcg
      421 cgcgtatggt gcaggccctg atcggtaaat gacgcgagtg cgagcgtatg aaccgcctcg
      481 ccgacacgtt ctttggacgc gagcattcgt ttcactgccg ggaatcggaa gattagaaac
      541 ccggcgaagg cgccgatgac cggccaaagt tgaaagtagg gcgaccccca cggatcgctc
      601 caaaaccatt cgactaacat gccggcgaac agcccgatga atacgccgag caattcgatc
      661 tctgtatagc gcgccgccga cgtgacgatc atcgggacga tctccccggc ggtcttcgac
      721 tcggcggcga cgacggactg tcgcagccgt tctttttcgt cttcggtgaa aaaagtttca
      781 gctttcatga ttaccaactg tcagaagaac cgccgccgcc gaagctgccg ccgccaccgc
      841 taaaagtatc ggagccgcca ccaccgccaa atccaccgcc gccccagccg cccggcccgc
      901 caaaaatgat cggcccctga tgccggccgc ggccgcccca catgttattg cgccggcgag
      961 cacccgtatt ggcgaacgcc atcaaaccga ggaccgctaa ggtgattgca atcatgattg
     1021 gagagtttct gttcgagccg cggttttgag ttttctggcg cgccgcctcg ggtagcggct
     1081 cactacgaag aaccttatcg acggcgttta taccggcgac tatgccgccg gcataatcct
     1141 gttggcggaa gtgcggcaca atgtagtcgg cggtgatgcg tttcgcgatg gcgtcgggta
     1201 atacaccttc gagaccgtag ccgacttcga ggcggagccg gcggtctttg accgcgacca
     1261 ccaagatcgc gccgttatca tgcccttttt tgccgatctt ccagctctcc gcaacgcgga
     1321 tgctaaaacc ctcgatatct tcgccgtcga gggtcgggat cgttaggacg gcaacctgat
     1381 gtccagtatc gcgttcgagt tgggcgagct gggtctcgag cgcctgggct tgactcgact
     1441 gcagcacgtt ggcgtagtcg ttgacgcggc cgcgcagggt tggcacgtcg agcgaccatg
     1501 ctagcggaaa cagcagtaac acaaaagccg cggcgagcag cgagcataat agtgtctttt
     1561 gaagttcctt cgatttcgca gcagggcgca acatggtgat cattcaactt cgcttatgag
     1621 tatgtttgcc aggtcgtcgt aaacttttct atccgtaaag atcgccgtga ggatttcgtc
     1681 gcaaccatgg tcggacttta aactatccgc aagcatttca acacccgatt gccttgccgg
     1741 gaatgttctc gtcaggaccc cagcgctgga tgcggcgcat attaccaacc tccggacgcg
     1801 ccgccaccgc cgaaaccgcc accaccgcca cgaaatccgc cactgaagcc acctcccgat
     1861 ccgccaactc ggcttcggct tccagcacta cctgaacttt tagatgaccc agcgctccag
     1921 gatcgccccc aagctcgccg ggcaagatgt accgggagca tccccgcaag cgcaccggtg
     1981 agaatcgtca atagccagac gccaacaccg atgacggaaa tggcccgcac accgatgaca
     2041 ccggatatcg ctgctcccat gaaagcggct cggccgaggg atggttgagt aaagcccaca
     2101 accgaaccga acaggaccgc gataaccagc aaggtgaaaa tcgccgacag cgacgatctt
     2161 ggcactttat gcaaggtcgg cggttcgtta agaccttgtt cacctttaat ggcttgaata
     2221 atcgccgatg tccctgattc aatgccgccg gcaaagtccc ggtcgcgaaa tcgtgggacg
     2281 atcacttcct gaataatccg gttggcaatt gcgtccggca gcacgccttc gagcccatac
     2341 ccgacttcga tgcgaatctt gcgatcgtcg cgagcgacga tcaggatcac cccatcgtca
     2401 gcgcctttgt gaccgatctt ccacgcttcg gcgacacgca tgctgaagtc ttcgagactg
     2461 tcgccattca aactgccaat cgtcagaacc gcgatctggt gatgcgactg gtcttcgaag
     2521 cgaataagcc gatcttcaag ttctgatacc tgggattcgc tcatcatgcc ggcgccgtca
     2581 ttgacccgcg cctgcaaggg tggcacgtcg cgcgcaagca cgaggtgcgc gttcagcgcg
     2641 aggaccagga acgcgagtaa aggagaccaa ggcactatgg cac
//
LOCUS       DASQKS010000479         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3634245, whole genome shotgun sequence.
ACCESSION   DASQKS010000479 DASQKS010000000
VERSION     DASQKS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3634245"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1016..1150
                     /locus_tag="VE170_12380"
     CDS             1016..1150
                     /locus_tag="VE170_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16282.1"
                     /translation="MQPEDWAKKYAAMDKRIELKYQQLAKGEQIGKRPDAPKTQPKKS
                     "
     gene            complement(1157..1699)
                     /locus_tag="VE170_12385"
     CDS             complement(1157..1699)
                     /locus_tag="VE170_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYJ16283.1"
                     /translation="MPRLPELNETKLSPEQKVIYDDIMRLRGRVRGPFAVWLRNAELA
                     GGALKLQELFASGVKMERRLIQLMILVAARYAGAQYAWYIHEPHALQHGIAREIVEAI
                     RARRTPDFTHEDERLVYDITLELNTTHRLSDSTFGRGMALFGEQQMVELVSGVGFYAM
                     VAMTLNAFDVAVPDGNKPLA"
     gene            1841..2215
                     /locus_tag="VE170_12390"
     CDS             1841..2215
                     /locus_tag="VE170_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016948842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYJ16284.1"
                     /translation="MDSRAKYRDIKERTFEFAVEIVKVCRLLDEKPGVNRTLGRQLLR
                     SGTSIGANVEEAQAGQSRADFISKYSIALKEARETIYWLRLLYASGDLVTGQFKPLIP
                     EAEEISRVIGTIIVNARKSPKH"
     gene            2338..>3056
                     /locus_tag="VE170_12395"
     CDS             2338..>3056
                     /locus_tag="VE170_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ16285.1"
                     /translation="MAQAGANGWDLEADVVVIGSGGAGMPAAVKARDEGASVIVVEAN
                     YDIGGHAIISGGNVPLGGGTSAQKKYGIEDSPDTVFSDLTDWTVVQNNGWPDFRYNDR
                     EVMRAFADHCAATFEFLLSNGVEFKPVPPDNQGGHNLGNSAPRENHAFWTKGAGYESP
                     NARPGTGVIRPLENSARAKGVKFLLNYKMTEFVREAQKSGAVLGITAAYTPRIMPGQS
                     TPLKSFRSDGNIDSTQPIMRVR"
BASE COUNT          739 a          804 c          856 g          657 t
ORIGIN      
        1 tggggtcgca gacggtgcgg actatgtggg attcagccat ggatggaaac tcacaactga
       61 gtaaatatcg ccagccgacc gactgcaagg cgtcggaggc catcctgcgg ttttaaattg
      121 tcctccttat tcccgttatc acgggacgac ggactaagtg ttttggccgg gtgaaccgag
      181 aaagctggcg gtagcgaaga agcgaaacca ttagcttgcc accttctcat gatgcacaaa
      241 gatcactctc catcaagggc ctttggtttc ccgcagcgct ggctttttga tactgggtta
      301 agtatgaact gtaccgttac ggaccgtatg gattcggcag gcgatgtgtt acatcggctg
      361 atcgccggag tgacatccgc gctcgctatg tataaaagca aggcttcgct ggattgctcg
      421 tccgggggag ggctctctca cgaaccaatg ctgtggattg cggcataacg ctgcggcaag
      481 agaattttga ggcccacgcg aattcacctc aaagaaggga aaagttttca gatcaccgta
      541 gcatgatcca atgtttggaa acaaatacgc ttaaaaattc atcacttcgc aacggagatc
      601 ggtggaatga tttctgcttc tgtcttgagt tcgtacgcta caagtgtgca acgatctcga
      661 atctctgtcg cgcatgaaat catatccgac agacggataa tggactgtat tgcggggatc
      721 gggatcgcca ctgggcttta ccgcaaaatg gcgcgccatg ttctcggtga attttgaacc
      781 tgtagtggtc gaaccatatc tgctgactgc ctgcggcctc actttatgga agatggttta
      841 cttcaaaggt cgagcgatca cgaccaacgt ccgtcggcag gaacccgaat tgcaagatcg
      901 cggtggcgat gcgcccgttt aaaggccgac ctaacccgga agcaactggc gaagcagagt
      961 gcaaaatacc cgacgagtaa tatcgctaac ggtgaataat tttaggaggc tagcaatgca
     1021 accagaagat tgggcgaaga agtacgcggc gatggataaa cgtatcgagc tcaagtacca
     1081 gcaactggcc aaaggcgaac aaatcggcaa gcgccccgat gcgcccaaaa cccaacccaa
     1141 gaagtcttaa cctactttac gccaacggct tattgccgtc ggggacggcg acgtcgaagg
     1201 cgttgagcgt catcgccacc atggcgtaga agcccacgcc ggagacgagc tcgaccatct
     1261 gctgttcgcc gaacagcgcc atgccgcggc cgaatgtcga gtccgaaagc cgatgggtgg
     1321 tattgagttc caaggtaata tcgtacacca accgttcgtc ttcatgggtg aagtccggcg
     1381 tgcgccgcgc gcggatcgcc tcgacaatct cgcgagcgat cccatgctgc agcgcatggg
     1441 gctcgtgaat gtaccaggca tactgcgcgc cggcgtaacg cgccgccacc aggatcatca
     1501 gttggatcag ccgccgctcc atctttaccc ctgacgcaaa caattcctgc agcttgagcg
     1561 cgcccccggc gagctcggcg ttgcgcaacc aaaccgcaaa cggcccgcgc acccttccgc
     1621 gcaggcgcat gatgtcatcg tagataacct tctgttccgg cgaaagcttg gtttcgttta
     1681 gctcgggtaa tcgcggcatg tttacctccg gcgatttagc tagtgcaata tgtatcacag
     1741 ttggagtctt gcgagttagc aaagtgattg cgtaaggcaa aagtaaaaag gtaaaaggca
     1801 aaacttaaat aggacttcca gacagaagga gtgcgagaca atggactcga gagcgaaata
     1861 tcgcgacatc aaggaacgga cgtttgaatt tgccgtggaa atcgtcaagg tctgccgttt
     1921 actggacgag aaacccgggg tgaatcgcac cttaggtcgc cagctcctac gttcgggcac
     1981 ttctattggc gcgaatgttg aggaggctca ggctggtcag agccgagcgg attttattag
     2041 taagtatagt atcgcattga aggaagctcg cgagacgatt tattggttgc gcctgttata
     2101 tgcgagtggt gatctcgtca cgggtcagtt caaacctttg atacccgagg cggaagaaat
     2161 ttcccgagtc atcggcacca ttatcgtcaa cgcaagaaaa tcaccaaagc actaacccgt
     2221 tttgcctttt acctttttac ttataccttt caatcggctt tgctcttgta attttcgacc
     2281 gtcggttgta atcgaatcta aatccacatt tgacagtcta aagcggagga tagttggatg
     2341 gcacaagcag gagcgaatgg ctgggatctc gaagcggatg tcgtcgtgat cggttccgga
     2401 ggagcgggca tgccggcggc ggtcaaagcg cgcgacgaag gcgcttcggt tatcgtcgtc
     2461 gaagctaact acgatatcgg cggtcatgca atcatcagcg gcggcaatgt gccgctcggc
     2521 ggcggcacca gcgcgcagaa gaaatacggc atcgaggatt ctcccgatac ggttttctcc
     2581 gatctcaccg actggaccgt cgtgcaaaac aatggctggc cggatttccg ctacaacgat
     2641 cgcgaggtga tgcgcgcctt tgccgatcat tgcgcggcga ctttcgagtt cctcctcagc
     2701 aacggcgttg agttcaaacc ggtcccgccg gacaatcagg gcggtcacaa cttgggcaat
     2761 tccgcgccgc gcgagaatca tgccttctgg accaaaggcg cgggctacga aagtcccaac
     2821 gcccgaccag gcaccggagt gattcggccg ctggaaaaca gcgcccgcgc caagggcgtg
     2881 aaatttttgt tgaattacaa aatgaccgaa ttcgtccgcg aagcgcagaa gagcggcgcg
     2941 gttctcggca tcactgcggc atatacgccg cggatcatgc cggggcaatc gacgccgctc
     3001 aagagttttc gttccgacgg taatatcgac agcacccaac cgatcatgcg cgtgcg
//
LOCUS       DASQKS010000480         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5716635, whole genome shotgun sequence.
ACCESSION   DASQKS010000480 DASQKS010000000
VERSION     DASQKS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5716635"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..3535
                     /locus_tag="VE170_12400"
     CDS             134..3535
                     /locus_tag="VE170_12400"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HYJ16286.1"
                     /translation="MNNGNYTVTLKQPGFFAFFCTQFLGAFNDNFLKIIVSFVALSLA
                     GHGGDFYVELIAILFILPSALFSGYAGHLADVYSKRTILVAVKAAEIAIMIFVLVSFY
                     FADIRPMLAAVFLMGLHSAFFSPAKYGILPEMLPDKELSRGNGLLEMSTFMAIILGTS
                     VGSAIFSVWKNQLPRIGLIMIAIAIAGYLTSLRIAKVPASGSTKLLNLNPFGEITDGL
                     RRLRRQRVPWFTVLGISYFWFLGALVNINILFFGKELLQLDEFHIGLLGTFLAIGIGI
                     GSLAAGKLSGDHIELGLVPLGSVGMGICLALVAWAAPSYPHTALALVLLGFAAGLFAV
                     PLNALLQQRSGKEEKGQLIATNNFLNTIGIALAAALHWFLKTPLHLSPDTIILLIGLF
                     TLAGTVAILYLLPDYFVRFVVWLLTNTLYRIRVVGGQNIPLQGPALLICNHVSFVDAL
                     LVGAPQPRFVRFMLHREYYDLKSLNWFFRLMHSIPVSANNRRDIVQSLKRARNELENG
                     HVVCIFAEGAISRTGRLLPFKRGFEKIVEGSNIPIVPVHLDQLWGSIFSFKDGKFFWK
                     WPMQFPYSVTVSFGAPLPPTSTVAQVRNAILELESEAVEYRRRPGDLLHAKFVEMAKR
                     HWSRFCMADTTGTELTYGKALIGSLLFCSWLRSHCAGQSMIGVILPASVGGALINIAI
                     LLAGKVPVNLNFTGGKEAMAAALSQCKITTIVSSRTFLEKANLEKIDGMVFVEEIRQT
                     FTGAGKLATAVKAYVTPAHWLNRRARKQQRPNDLATIIFSSGSTGTPKGVMLSHHNII
                     SNVEAIGQVIQFTPQDRIIGVLPLFHSFGFTATIWLPVLTGFGAVYHPNPSDAKIIGE
                     IVEKYRATLMISTPTFYVSYWRRCTKEQFASLRYVIAGAEKLREQIAQGYQDKFGITI
                     LEGYGCTELSPVVAVNTPDVIHDKEKQTGHKPGTVGQPLPGVATKVIDPDSGEPLGPG
                     AEGLLLVKGPNVMLGYLNQQDLTDQAIRQGWYVTGDIATIDEDGFIKITDRLSRFSKI
                     GGEMVPHMRIEEMINEVLGSPDSVVTAIPHEQRGERLVVFYAKNGVSREELWDKLNKS
                     ALPKLWIPKRDDLHAIDEIPILGSGKADLKRVKAMALEKTKQAAT"
     gene            3583..>4176
                     /locus_tag="VE170_12405"
     CDS             3583..>4176
                     /locus_tag="VE170_12405"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ16287.1"
                     /translation="MEMTMVGAQQIVKYLEHATDDFKQSREYRDLVDGAAGRAPAYEF
                     LRNIFRTHYLSAHIVALCFAALPSASAKILQENLLEEMGRSEHEPPHSELLLEMARAI
                     GFTDDEIDRLIADARRRVALVCATRVPVATLRELCLSVLLETMSFEFMLSRCSSELAN
                     ALSDKYAVPRAALRWFELHSEVDIRHAEEAITVIQDYS"
BASE COUNT          843 a         1269 c         1160 g          904 t
ORIGIN      
        1 ccatgggcgg attatgttta tttcgtcccg ctttcgcaag cgcttggttg tacgagacgc
       61 gagcgaattg acggtgttgg cctggcttcg accttgccga accgcgcgga gccgagtaaa
      121 ttagccagca ataatgaata acggcaacta caccgtcacg ctcaaacagc cgggattctt
      181 tgctttcttc tgtacccagt tcttgggtgc attcaatgac aattttctaa aaatcatcgt
      241 ctcgttcgtc gccctgagct tggccgggca cggcggcgat ttctacgtcg aactgatcgc
      301 catacttttt atcctgccgt cagcgctgtt ttccggttat gccggccatc tcgccgacgt
      361 ttacagcaag cggacgatcc tggtggcggt gaaagcggcc gagatcgcca tcatgatttt
      421 cgtcctggtg tcattctact tcgcagacat ccggcctatg ctcgcggcgg ttttcttgat
      481 gggcctgcac tcagcattct tcagcccggc caaatacggc atcttgccgg agatgctgcc
      541 ggataaggaa ctgtcgcgtg gtaacggctt gctggagatg agcaccttca tggcgatcat
      601 tctggggacg tcggtgggca gcgcgatctt ctcggtctgg aaaaatcagt tgccgcgcat
      661 cggcctgatc atgatcgcca ttgctatcgc cggttatctt accagcctgc gcatcgccaa
      721 ggtgccggct tcggggtcga ccaagctgct caacttaaac cccttcggcg aaattaccga
      781 cgggctgcgc cggctgcgcc gccaacgggt gccgtggttt acggtcctcg gcatttccta
      841 tttttggttt ctcggcgcgc tggtcaacat caatattctc tttttcggca aagagctgtt
      901 gcagctcgat gagtttcaca tcggcctgct cggaactttc ttggcgatcg gcatcggcat
      961 cggcagtctc gccgccggca aactctccgg cgaccatatc gaattgggct tggtacccct
     1021 cggctcggtg ggcatgggca tttgcctcgc cctggttgcc tgggcggcac cgtcgtatcc
     1081 gcacaccgca ctggccttgg tgctgctcgg ctttgccgcc ggcctgttcg ccgtgccgct
     1141 caacgcgctg ctgcaacaac gcagcggcaa agaggaaaag ggccaactga tcgcgaccaa
     1201 taatttcctg aacaccatcg gcattgccct ggccgcggcg ctgcattggt tcttgaaaac
     1261 gccgctccac ctatcccccg acaccatcat cttgctgatc ggactgttta ccctggccgg
     1321 cacggtggcg atcttgtacc tgctgcccga ttatttcgtc cgcttcgtgg tctggctgtt
     1381 gaccaacacg ctttaccgca tccgggtggt cggcggacaa aatatcccgc ttcaagggcc
     1441 ggcgctcttg atctgcaacc atgtctcctt tgtcgatgcg ctcttggtcg gcgcgccgca
     1501 gccgcgcttc gtccgcttca tgctgcatcg ggagtattac gatctcaaat cgctcaactg
     1561 gttttttcgc ctcatgcact cgatcccggt gtcggcgaac aatcgccgcg acatcgtcca
     1621 gtcgctcaag cgggcgcgaa atgaactcga aaacggtcat gtggtgtgca tcttcgccga
     1681 aggcgcgatc agccgtaccg ggcggctgct gccgttcaag cgcggcttcg aaaaaatcgt
     1741 cgagggttcg aatattccaa tcgtaccggt gcacttggat cagctctggg gcagcatctt
     1801 tagttttaaa gacggcaagt tcttttggaa gtggccgatg caattcccct attcggtcac
     1861 ggtctcgttt ggcgcgccac tgccgccgac ctccaccgtg gcccaggtgc gcaatgcgat
     1921 tctagagctg gaaagcgagg cggtggagta ccgtcgtcgt cccggcgatc ttctccacgc
     1981 caagttcgtc gagatggcca agcgccactg gtcgcggttc tgcatggccg acaccaccgg
     2041 cacagagctc acctatggca aggcgctcat cggtagcctg cttttttgca gctggctaag
     2101 aagccattgc gccggccagt cgatgatcgg cgtgatctta cccgccagcg tcggcggcgc
     2161 gctgatcaac attgccatct tgctggcggg caaagtaccg gtgaatttga atttcaccgg
     2221 cggcaaggaa gccatggccg cggcgctgtc ccaatgtaag atcaccacca tcgtcagttc
     2281 gcgaacgttt ttggagaaag ccaatctaga aaaaatcgac ggcatggttt tcgtcgaaga
     2341 gatccgccag acatttaccg gcgcgggaaa actcgccacc gcggtcaaag cctatgtcac
     2401 gccggcgcat tggctcaacc gccgcgcccg taaacaacaa cgccccaatg atctcgccac
     2461 gatcatattt tccagcggca gcaccggcac gccgaaaggc gttatgctgt cgcaccacaa
     2521 tataatttcc aacgtcgagg cgatcggcca ggtgattcag ttcacgccgc aggatcgcat
     2581 cattggtgtc ctgccgctgt ttcactcctt cggcttcacg gcgacaatct ggctgccggt
     2641 gctcaccggt ttcggcgcgg tctatcaccc gaatccctca gacgcgaaga tcattggtga
     2701 gatcgtcgag aaatatcgcg cgacattaat gatcagcacg ccgaccttct acgtcagcta
     2761 ctggcgtcgc tgcaccaagg aacagttcgc ctcgctgcgt tacgtcatcg cgggcgcgga
     2821 aaagctccgc gagcagatcg cccagggtta ccaagataaa ttcggcatca ctatcctcga
     2881 aggctatggc tgcacggaac tctccccggt ggtcgcggtc aacacacccg atgtcatcca
     2941 cgataaggaa aaacagaccg gtcacaagcc cggcacggtc gggcaaccgc tccccggtgt
     3001 cgcaaccaag gtgatcgatc ccgacagcgg tgagcccctg ggcccaggcg ccgaagggct
     3061 gctactcgtc aaaggaccca acgtcatgct tggctacttg aatcagcagg acctaaccga
     3121 tcaagcgatt cgccagggct ggtatgtcac tggtgatatc gcgaccatcg acgaggacgg
     3181 cttcatcaaa atcacagacc gcctgtcgcg cttcagcaag atcggcggcg aaatggtgcc
     3241 gcatatgagg atcgaagaga tgatcaacga ggtcctggga agcccggatt cggtggtgac
     3301 ggcgatcccg cacgaacagc gcggtgagcg gctggtggtt ttctacgcca agaacggcgt
     3361 cagccgcgaa gagctgtggg acaagctcaa caaatcggca ttgcccaagc tgtggattcc
     3421 caagcgcgac gatcttcatg ctatcgacga gattcccatc ctcggctcag gcaaagccga
     3481 tttgaagaga gtcaaagcga tggcgttgga aaaaactaag caggcagcca cataggctgt
     3541 ccgccgttgc tgcagcttgg taacagaacg gaaccattag cgatggagat gaccatggtc
     3601 ggcgcccaac aaatcgttaa atacctcgag cacgcaaccg acgatttcaa gcaaagccgc
     3661 gaatatcgcg acctcgtcga cggcgccgcg ggcagggcgc ccgcctacga gtttttgcgc
     3721 aatatttttc gcacccatta tctttcggcg catatcgtcg ccctgtgctt cgccgcgctg
     3781 ccatcggcgt ccgccaaaat tttgcaagaa aacctgctcg aagaaatggg ccggtcggaa
     3841 catgagccgc cccactccga gctgctcttg gagatggccc gcgccatcgg cttcaccgac
     3901 gatgaaattg atcgactgat cgccgacgcg cgccggcgcg tcgcgctggt gtgcgccacc
     3961 cgcgtgccgg tggcgactct gcgggaactt tgtctgtcag tattactcga aaccatgagc
     4021 ttcgagttca tgctgtcgcg ctgctcaagc gaactggcca acgctctgag cgataaatac
     4081 gccgtcccgc gcgccgccct gcgctggttc gagcttcatt cggaagtcga catccgtcac
     4141 gccgaagaag cgatcacggt gatccaagac tacagc
//
LOCUS       DASQKS010000481         7483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5718656, whole genome shotgun sequence.
ACCESSION   DASQKS010000481 DASQKS010000000
VERSION     DASQKS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5718656"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1061)
                     /locus_tag="VE170_12410"
     CDS             complement(33..1061)
                     /locus_tag="VE170_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYJ16288.1"
                     /translation="MKDEKTQIANRVEEIGVNQIEFNDPRLFRDLLGQHDQHVKILQN
                     TLQLKIRVRGSAMEIEGDPLQVELASQILRQLYSLLEKGYPVYASDVDYAIRILSGDN
                     RAKLQDIFLDTIYISAHKRTITPKSVAQKAYIDAIRRYDIVFGIGPAGTGKTYLAMAM
                     AVAELMKNNYVRIILTRPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALHDMVDFDRAR
                     KMVERGTIEVAPLAFMRGRTLNDSFVILDEAQNTTPEQMKMFLTRLGYGSKAVITGDA
                     TQIDLPAGKASGLKEAARILRGVHGIRFITFTEKDVARHHLVQEIIAAYERDDNQNNA
                     RDLLLEKT"
     gene            complement(1058..1486)
                     /locus_tag="VE170_12415"
     CDS             complement(1058..1486)
                     /locus_tag="VE170_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYJ16289.1"
                     /translation="MITIHTKTITESTAGCCDIIDITAKLKEQIRAVGMRLGTATLFV
                     NGSTAALTTIEYEPGLIQDLQEFLEKHIPTDRRYHHDDRWNDDNGFSHLRASLFGPSL
                     AIPIIDGAPSLGIWQQVTLLDFDNRPRNREVIVQLIGECE"
     gene            complement(1589..4222)
                     /gene="alaS"
                     /locus_tag="VE170_12420"
     CDS             complement(1589..4222)
                     /gene="alaS"
                     /locus_tag="VE170_12420"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYJ16290.1"
                     /translation="MTANDIRKSFLDYFAKRGHTVVRSSSLVPDKDPTLLFTNAGMVQ
                     FKNVFLGQQRLPYVRAASAQKCLRISGKHNDLEVVGRDTYHHTLFEMLGNWSFGDYYK
                     AEAIEWAWELLTKEWGLPKDKLYATVYLNDDDAEQLWFKISGLPRERVGRFGEKENFW
                     EMGETGPCGPCSEIHLDRGPAACDRQGIAGHQCKVNGDCARFIELWNLVFIQFNRAED
                     RSLSELPAKHVDTGMGMERITAVLQNVLSNYDIDIMRDLTAATERLTGKRYGVDPTAD
                     ISFRVIDDHSRAVSFLIADGVSPSNEGRGYVLRRLLRRAARHGRLIGLKEPFLHEVAK
                     TVAAVMGDAYPELRQEEQRIREVIRSEEERFGETLDRGLILLEDATAKLKAEQKRTLS
                     GEVAFRLYDTYGFPLDLTEDILRADDIDVDQQSFTELMEAQRNRGREARESVSMESKI
                     QLDGQVRFTGYDRLDGESSVIGIFGNSGSKIEALEGDEIEVLTAETPFYGESGGQVGD
                     RGVIRTARGDLIEIIDTQHPTPQLTSHRGRVKKGRIQVGDRVELHVDGKYRQKTMLNH
                     SATHLLHAALRDELGQHVRQAGSLVTPERLRFDFSHMGAIADEKLAAIEAQVNQHVRA
                     NARVSIQELSYDEAIRHGALAFFGDKYGDRVRVVKIGDFSTELCGGTHIRSSGEIGLF
                     KLNFEGGVAAGVRRIEAFTGEGALDLIYRYESRLKEIGALIRGNADDAVDKVKRLLDR
                     QKELEREITKLRGELEKDRIPDLLAKKQTFGDANALIAQVDGVDGKQLREIADQLKEK
                     LGPGVVVLASASDGNVNLVATVSKELTKKFHAGNIVKELAGMVGGGGGGRPDFAQAGG
                     KEPEKIAAALERAGQIVKQAT"
     gene            complement(4232..4675)
                     /locus_tag="VE170_12425"
     CDS             complement(4232..4675)
                     /locus_tag="VE170_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HYJ16291.1"
                     /translation="MQLALGYLSYRPRSEFEVKNYLRQKGCEPQVSDAVLAKLRSLHY
                     LDDPSFAQIWARSRLESRGYGPRRVEQELRLRGIDAKVIRETIQQSLQRTSEQESARR
                     ILTKRFGGGSLVDAKARRRAVALLQRRGFSSRVIFDLLQYRIDED"
     gene            complement(4747..5799)
                     /gene="recA"
                     /locus_tag="VE170_12430"
     CDS             complement(4747..5799)
                     /gene="recA"
                     /locus_tag="VE170_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HYJ16292.1"
                     /translation="MDANREKAIDLAISQIEKQFGKGAIMKLGEGGIVKDVQTISTGS
                     LGLDIALGIGGVPCGRVVEIYGPESSGKTTLALQIVAEAQKSGGMGAFVDAEHALDLG
                     YAQKLGVKTDDLLVSQPDHGEQALEIAETLVRSGAIDVLVIDSVAALVPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTGTIARSHTVVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRMDIRRIGALKDGDNIVGGRTRVKVVKNKMAPPFKEAEFDILYGTGISRDGEIVD
                     IGSEIGVIEKSGAWYSHGGERIGQGREQAKQFLKDHPETAQNIMAKIMDKYGLKPHDA
                     EALSEPAPENKKSKTK"
     gene            complement(5809..6381)
                     /gene="thpR"
                     /locus_tag="VE170_12435"
     CDS             complement(5809..6381)
                     /gene="thpR"
                     /locus_tag="VE170_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYJ16293.1"
                     /translation="MPRAFIAVEVAPATVASVASAIAQLKPKLPGIRWVPPANFHLTV
                     KFLGDIEASRVEAVAAALQPALAPFPRCTINAKGLGVFPGLRQAKILWVGFFGDELAR
                     LAASIDRALLALEFSAEPRAFTPHLTIGRRRHVDPPAVNLKQELPLWRDRDFGATIVD
                     RVILFQSVLEPTGAVYSPLKVIKLNDQSID"
     gene            complement(6392..>7483)
                     /locus_tag="VE170_12440"
     CDS             complement(6392..>7483)
                     /locus_tag="VE170_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HYJ16294.1"
                     /translation="WALSQAQELGDIVIGTGGLGPTTDDLSTELIADFVGLKTKLDDG
                     VAAALKARFARRGVAFTQNNLKQAFFPEGAEIIPNPNGTAPGFRVDLGNGKRLIWLSG
                     VPHEMSAMLSESVLPWVVEQNGSQATIHEATFKIHGISEAKLDDLVKPLDLGNFAKLS
                     FRAHFPDLTLRLTMSSGAARTEHFLNLRGQIKTILDAYIYADSDLTMEEVVGQLLLRR
                     QQTLALAESCTGGLISHRITRVAGSSAYFLGAAITYSNAEKIRALGVNPATLDRAGAV
                     SQDTALEMSQGVRQRTGAAIGLSVTGVAGPSGGSADKPVGTVWVGICRDNFHQAKLLN
                     LMVLSDRDRIVQGTAQAALNWLRQLLLVE"
BASE COUNT         1424 a         2179 c         2168 g         1712 t
ORIGIN      
        1 ccatcggcgc gccgatcggc agatagttcc ggttaagttt tttcgagcag cagatcgcgg
       61 gcgttgttct gattgtcgtc ccgctcatag gcggcgatga tctcttgaac caagtgatgg
      121 cgcgccacat ccttttcggt aaacgtgata aaccggatgc catgcacgcc gcgcaaaatt
      181 cgcgccgctt ctttgagtcc cgaggccttc cccgccggca aatcgatctg agtcgcgtcg
      241 ccggtgatca ccgcctttga gccgtaaccc aatcgggtca gaaacatctt catctgctcc
      301 ggcgtggtgt tctgtgcttc gtcgagaatt acgaaggaat cgttaagtgt gcggccgcgc
      361 atgaacgcca gcggggctac ttcaatggtc ccgcgctcga ccattttgcg ggcgcggtcg
      421 aagtcgacca tgtcatgcag cgcgtcatag agcgggcgca gatagggatt gaccttttcc
      481 gccagatcgc cgggcagaaa tcctaatttt tccccggctt cgaccgccgg tcgggtgaga
      541 atgatgcgca cgtaattatt cttcattaac tcggccacgg ccatcgccat ggcgaggtag
      601 gtcttgccgg tgccggcggg accgatgcca aagacgatgt cgtagcggcg gatcgcatcg
      661 atataagctt tttgcgcgac gcttttcggc gtgatcgtgc gcttgtgcgc ggaaatatag
      721 attgtgtcaa gaaatatatc ttgcagcttg gcgcggttat cgccgctcaa gatacggatc
      781 gcatagtcga cgtcgctggc gtaaaccgga taaccttttt ccagaagact gtagagttgc
      841 cgcaatattt ggctcgccag ctcgacttgc agcgggtccc cttcgatctc catcgctgaa
      901 ccgcgtacgc ggattttgag ctgcaaggtg ttctggagga ttttgacatg ctggtcatgc
      961 tggccgagca gatcacgaaa caaacgcgga tcgttaaact caatttggtt gacgccgatt
     1021 tcctcgactc gattggcgat ctgagttttt tcgtccttca ctcgcattct cctatgagct
     1081 gcacaatgac ttcgcggttg cgcggccgat tgtcgaagtc caataaggta acctgctgcc
     1141 aaattcccag actgggcgcg ccgtcgatga tcgggatcgc caacgacggc ccgaatagcg
     1201 aggcgcgcag atgagaaaaa ccgttatcgt cgttccagcg gtcgtcgtgg tgataacgcc
     1261 ggtcggttgg aatatgtttt tccaggaatt cttgcaaatc ttggatcagg cccggctcat
     1321 actcgatagt tgtcagcgct gcggtggagc cattgacaaa cagcgtcgcc gtgccgaggc
     1381 gcatgcccac ggcgcggatt tgctccttca atttggcggt gatgtcgatg atgtcgcagc
     1441 agccggcggt ggattcggtg atcgtcttgg tgtgaatcgt gatcacgcta gcaatcgacc
     1501 cgtggcggct gagacagatt gatactccga tagtaaaggc aagccggcgt cggtggctgt
     1561 cttccctgac tgcttggctt cgcaaatttc atgtcgcctg cttaacgatc tgcccggcgc
     1621 gttcgagcgc ggcggcgatt ttctccggct ccttgccgcc ggcctgggcg aaatccggcc
     1681 gccctccacc acctccgccg accatgccgg cgagttcttt aacgatgtta cccgcgtgaa
     1741 actttttggt caattccttg ctgaccgtcg cgaccaagtt gacgttgccg tcgctggcgc
     1801 tggctaggac aacgacgccg gggcccagtt tctccttcag ctgatcggct atctcgcgca
     1861 actgcttgcc gtcgacgccg tcgacctggg cgatcaaagc attggcatcg ccaaaggttt
     1921 gtttcttggc cagcaaatcg ggtatgcgat ccttttccag ctcgccgcgc aacttggtga
     1981 tttctcgttc caattctttc tggcggtcga gcagtctttt tactttgtcg acggcatcgt
     2041 cggcgttgcc gcgaatcagc gcgccgattt ctttgagacg cgactcgtat cggtagatca
     2101 aatcgagcgc gccctcgccg gtaaaggctt cgatgcgcct gacgccggca gcgacgccgc
     2161 cctcgaaatt gagcttgaac aatccgatct caccggaaga gcggatatgt gtgccgccgc
     2221 aaagctcggt ggaaaaatcg ccgattttga cgacgcgcac gcggtcgcca tacttatcgc
     2281 cgaaaaacgc cagagcaccg tggcggatcg cttcgtcgta actcaactcc tggatcgaca
     2341 cccgggcatt ggcgcgcaca tgctggttaa cttgcgcctc gatggccgcg agtttttcgt
     2401 ctgcgatcgc tcccatatgg ctgaagtcaa aacgcagccg ctcgggcgtc accaaggaac
     2461 cggcttgacg cacatgttgg cccagttcat ctctcaatgc ggcgtgaaga agatgggtcg
     2521 ccgaatggtt gagcatggtt ttctggcgat atttgccgtc gacgtgaagt tctaccctgt
     2581 cgccgacttg gatgcggcct tttttcactc tgccgcgatg ggaggtcagc tgcggcgtcg
     2641 gatgttgggt atcgataatt tcaattaaat cgccgcgcgc ggtcctgatc acaccgcggt
     2701 ctcccacctg cccgccggat tcaccgtaaa aaggcgtctc ggccgtcagg acctcgatct
     2761 cgtcgccttc gagcgcttcg atcttgctgc cggagttacc gaagatgccg atgaccgacg
     2821 attcgccgtc gaggcgatcg tagccggtga aacggacctg gccatcgagt tggatcttcg
     2881 actccatgct gacagattcg cgggcctcac ggccgcggtt ccgttgcgct tccataagct
     2941 cggtgaaact ctgctggtcg acgtcgatat cgtcggcgcg caaaatgtcc tcggtgaggt
     3001 cgagggggaa gccgtaggta tcgtacaggc gaaaggcgac ctcgccggat agggtccgtt
     3061 tttgctccgc tttgagttta gccgtggcgt cttccaggag aatcaagccg cgatcgaggg
     3121 tttcgccgaa gcgctcttct tcgctacgta taacctcgcg gatgcgctgc tcctcttgcc
     3181 tcaattccgg ataggcatcg cccatcaccg ctgcgaccgt tttggcgacc tcatggagaa
     3241 agggttcttt caagccgatc aagcggccat ggcgcgcggc gcggcgcaac agacggcgca
     3301 acacatagcc gcgcccttcg ttgctcggcg aaacgccgtc ggctatcaag aaactgacgg
     3361 cgcgggagtg atcgtcgatg acgcggaagg aaatatccgc ggtcggatcg acgccatatc
     3421 ttttacccgt gagcctttcg gtggctgcgg tgagatcgcg catgatgtca atatcgtaat
     3481 tcgacaggac gttttgcagc accgcggtga tacgctccat gcccatgccg gtgtcgacgt
     3541 gtttagccgg cagttccgat aagctgcggt cctcggcacg gttgaactga ataaaaacca
     3601 ggttccacag ctcgatgaaa cgggcgcagt cgccgttaac tttgcattga tgaccggcga
     3661 tgccttgcct atcacaggcc gccggcccgc ggtcgaggtg aatctcactg cagggaccgc
     3721 aggggccggt ttcgcccatt tcccaaaagt tttctttttc gccgaagcgg cctacccgct
     3781 cgcgcggcaa gccgctgatc ttgaaccaga gctgctccgc gtcatcgtcg ttcaaataga
     3841 cggtggcgta aagcttatct ttcggcaggc cccattcttt ggtcaacagc tcccaggccc
     3901 actcgatggc ttcggctttg tagtagtcgc cgaacgacca gttgccaagc atctcgaata
     3961 gcgtgtgatg gtaggtatca cggccgacga cctccaagtc gttgtgtttg ccgctgatgc
     4021 gcagacattt ttgcgcgctg gcggcgcgca catagggcaa acgctgctgg cccaagaaga
     4081 cgttcttgaa ctgcaccatg ccggcgttgg tgaacagcaa cgtcgggtcc ttgtcgggca
     4141 ccaaggacga gctgcgcacc acggtgtgac cccgtttggc gaagtaatct aaaaaagatt
     4201 tgcggatatc gttagcggta atcatagctt tctagtcttc gtcgatccga tattgcagca
     4261 ggtcgaagat cactctactg ctgaaaccgc gccgctgcag cagggcgacg gcgcggcgcc
     4321 gtgccttggc atctacaagg ctgccgccgc cgaatcgttt agtcaatatt cttcgggcac
     4381 tttcctgttc gctggtgcgc tgtaaacttt gctgtatcgt ttcccgaatc actttggcgt
     4441 cgatgccccg gaggcgtaac tcctgctcga ctcttcttgg gccataaccg cggctttcca
     4501 gccgagagcg cgcccagatc tgcgcaaagg acgggtcgtc gaggtagtgc agcgaccgca
     4561 gcttggcgag cacggcatcg ctaacctgcg gctcgcagcc cttctggcgc aggtagttct
     4621 tgacttcaaa ctcgctgcgc ggccggtagc tcaagtagcc caaagccagt tgcaaagcct
     4681 gctcgggcga gtcgacggcc gcggcgctgc catcttttcg tttcagcgtc tatccaaatg
     4741 ctaagattat ttcgttttac tttttttgtt ctctggcgct ggttcgctca gggcttccgc
     4801 atcgtgcggc ttcaatccgt acttatccat gatcttggcc atgatattct gcgccgtttc
     4861 cggatgatct ttgagaaact gcttggcttg ctcccgccct tggccgatgc gttcgccgcc
     4921 gtgggagtac caggcgccgc ttttctcgat gactccgatc tcgctgccga tatcgacgat
     4981 ctcgccgtcg cgggaaatcc cagttccgta aagaatgtcg aactcagctt ctttgaaagg
     5041 cggcgccatc ttgttcttga ctaccttgac gcgggtgcgc ccgccgacaa tattatcgcc
     5101 gtccttgagc gcgccgatgc ggcggatatc catgcgcacc gaggcgtaga acttgagcgc
     5161 attgccgccg gtggtggtct ccgggttgcc gaacatgacg ccgatcttca tgcgaatttg
     5221 gttgatgaag atgaccacgg tgtgtgagcg ggcgatggtg ccggtgagtt ttcgcaacgc
     5281 ttgcgacatg aggcgggctt gcaaccccat gtgcgagtcg cccatctcgc cctcgatctc
     5341 ggctttgggc accaaagcgg cgaccgagtc gatgaccaag acatcgatgg cgccgctgcg
     5401 caccaaggtc tcggcgattt ccaaggcctg ctcgccatga tccggttgtg acaccagtaa
     5461 atcatcggtc ttcacgccga gcttttgggc gtagccaaga tccagggcat gttcggcgtc
     5521 gacgaaagcg cccatgccgc cgctcttctg ggcttcggcg acgatctgca gcgccaaggt
     5581 agttttaccg gaggattccg ggccgtagat ctcgaccacg cggccgcagg gtacgccgcc
     5641 gataccgagc gcgatgtcga gtcccaggga gccggtcgaa atggtctgca cgtctttgac
     5701 aatgccgccc tcgccgagct tcatgatcgc gcctttaccg aactgttttt cgatctgact
     5761 tatcgccaga tcgatcgcct tctcccgatt tgcatccatt cctgcctcct agtcgattga
     5821 ctggtcgtta agcttaatca ctttcagcgg cgaataaacc gcccccgtgg gctcgagcac
     5881 gctttggaat aagatcaccc gatcgacgat cgttgcgccg aaatcccggt cgcgccagag
     5941 aggcaattct tgtttgagat taaccgccgg agggtcgacg tggcgccggc ggccgatggt
     6001 tagatgcggc gtgaatgctc ttggttcagc ggaaaattcc agcgctaaca gcgctctatc
     6061 gatgcttgcc gccaaccgcg ctaactcgtc tccgaaaaat cccacccaca aaatttttgc
     6121 ttgtctcagc ccggggaata cccctagacc tttagcattt atggtgcagc gcggaaaggg
     6181 ggcgagggcc ggttgcagcg ctgcagcgac ggcttcgacc ctgctcgcct cgatgtcgcc
     6241 gagaaacttg acggtcagat ggaagttcgc cggtggcacc cagcggatac ccggcagttt
     6301 gggtttcaac tgggcgattg ccgaggcgac gctggcgact gtcgcgggcg cgacttcaac
     6361 ggcgataaag gcacggggca tgacctactt actactcaac taaaagcagt tggcgcaacc
     6421 aattcaatgc cgcttgggcg gtgccttgca cgatccggtc gcggtcgctc aggaccatga
     6481 ggttaagcag tttggcttgg tgaaagttgt cccggcagat acccacccaa acggtgccga
     6541 ccggtttgtc ggcactgccg ccggatggac ccgctacccc ggtgacactg agcccgatgg
     6601 cggcgccggt gcgctggcgg acaccttggg acatttccaa ggcggtatct tgactgaccg
     6661 cgccggcccg atccaatgtt gccggattga cacccaaggc acgaatcttc tcagcgttag
     6721 aatatgttat tgcagcgccg agaaaatagg ccgagctgcc ggccactcgg gtgatgcgat
     6781 ggctgatcaa tccgccggtg caagactcgg ccagggcaag ggtctgctgc ctgcgcagca
     6841 gcagctggcc gaccacttct tccatggtta ggtcgctgtc ggcataaatg taggcgtcga
     6901 ggatggtttt gatctgcccg cgcaggttta aaaaatgttc cgtccgcgcc gcaccactgc
     6961 tcatggtcag acgcagagtt agatcgggga aatgagcgcg aaaggacagc ttggcgaagt
     7021 tgcccaaatc cagcggctta accagatcgt ccaatttcgc ttcgctaatg ccatgaattt
     7081 tgaaagttgc ctcatgaatc gtcgcctgac tgccgttttg ttcgacgacc catggcagga
     7141 ccgactcgct cagcatggca ctcatctcgt ggggcacgcc ggaaagccag atcagcctct
     7201 tgccgttacc aagatcaacg cggaagccgg gcgccgtacc gttcggattg ggaattattt
     7261 ccgcgccttc gggaaagaac gcttgtttga gattgttctg cgtgaatgcc acgccgcgcc
     7321 tggcgaagcg cgctttgagc gcggcggcga cgccgtcgtc tagtttagtc ttgaggccaa
     7381 caaaatcagc gatcagctcg gtggataaat catccgttgt cggccccaaa ccgccggtgc
     7441 cgatgacgat gtcgccgagc tcctgtgctt gactcaaggc cca
//
LOCUS       DASQKS010000482         7319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6151545, whole genome shotgun sequence.
ACCESSION   DASQKS010000482 DASQKS010000000
VERSION     DASQKS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6151545"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VE170_12445"
     CDS             <1..598
                     /locus_tag="VE170_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ16295.1"
                     /translation="QSKQERIPDLFCAALVGNFRGYFPGYSKLIAERQNYLSWIVLKA
                     HTINRAGTVTLQSADPRQPPNIDFRYFDEGTDRQGKDLDAVVEGIHFVRAMTKDLRER
                     GLIAAEEVPGEQYQSDDELREFVRDNAWGHHASCSCAIGPRDQDGVLSSDFRVHGTQG
                     LRVVDASVFPRIPGFFIVSAVYMIGEKAADVILAGANN"
     gene            915..1793
                     /locus_tag="VE170_12450"
     CDS             915..1793
                     /locus_tag="VE170_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ16296.1"
                     /translation="MSSIPAEFYALANAFLFAVHNIFMKKALRHSNPATGVISTLLIN
                     IVFLWTIAILFVPMANVTVASLLIFVGVGFFQPGLTRLLTYKGIDALGVAITDPIRAS
                     TPLFSAAMAIVFLGEEITLPIIVATLMIIGGITLLSWRRGAMKLAGSAVYLWFPIAAS
                     VLAGASQVVRKFGMAAVPHPFLAAAVTATSSLIISVLVMWYVEKTQETWKINRQCVWW
                     FIAAGITVSFAMTSIYHALDLGKVSVVIPLSSTAPLFSLILTALFLRDVERVTLRIAI
                     STMLIVSGVALISWWK"
     gene            complement(1897..2475)
                     /locus_tag="VE170_12455"
     CDS             complement(1897..2475)
                     /locus_tag="VE170_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ16297.1"
                     /translation="MNANIVRALTVAYLLLVAATVNAAAVGERLAPCLACHGENGQSV
                     IPEVPSLGAQPVFFLSVQLLMFREKMRNLEPMTSMMQGWSDDDLRRAAAIIAKLPAPR
                     ASTNAPDTDRAEKARVLIQQHRCNFCHKPDFAGEDNAPRLAGQREEYLVKSLREYKNN
                     SRRAYDPAMADALHPLTDADFQDLAHYLSRLN"
     gene            complement(2540..3796)
                     /locus_tag="VE170_12460"
     CDS             complement(2540..3796)
                     /locus_tag="VE170_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ16298.1"
                     /translation="MTSFPTLSRTAAVAAILGVLSMGAPALSQEKPAPNVQPAPSMVP
                     GRPDIPNAEVAKTLRGVAQPPFAAPAEKLQISQLKMPKGFKLEVYISGIANARSLRVG
                     DKGTVFVSNRQLDKVYAVVERNGKREAKVIASGLDRPNGLAFHNGSLYIGEGTKISKL
                     EKIEDNLDNPGKPVVIYTDLPNHQSHGWKFIAIGPDNKLYINVGAPCNICEPPAENAQ
                     IRRINLDGSGAEVFARGVRNSVGFDWHPITKDLYFTDNGRDWLSEDLPEDELNRVTKV
                     GQHFGFPYCHQGNFTDREMGWGRSCDEFTPPVALMGPHSAALGLRFYTGSMFPREYRN
                     ALFIARHGSWNRTKKIGGDVVVATLNVDGTIKSVQPFITGFLNNNEYWGRPVDVHVMK
                     DGSMLISDDFAGAIYRVTYSPQLAGR"
     gene            complement(3926..4438)
                     /locus_tag="VE170_12465"
     CDS             complement(3926..4438)
                     /locus_tag="VE170_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HYJ16299.1"
                     /translation="MRTLALIVALVATAGGAVAATPDMPKRKSGLWEITVSSDGAKGG
                     QTMQQCVDEKSDDLMKNSMAGTEKQSCSKNEFRREGAKLITESVCKMSGSTATTRGVF
                     TGQFDSSYKADIKSTYEPPLHGMKESAATMEAKWLGACKPGQKPGDISMPGMPNININ
                     DMMKNLPKRP"
     gene            complement(4485..5465)
                     /gene="bioB"
                     /locus_tag="VE170_12470"
     CDS             complement(4485..5465)
                     /gene="bioB"
                     /locus_tag="VE170_12470"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HYJ16300.1"
                     /translation="MNFQQLADKALQGILPEREELLEVLNAPDEQLPEILSAAFKVRH
                     HYYGKRVQIHVLQNAKSGLCPEDCHYCSQSSVSEAPVEKYTFMAKDKLVESARQAKAA
                     GAVRFCIVNSGRGPTNKEIDDITEAVREIRAQTGMNVCCSLGLMNNDKTRKLAAAGVG
                     RVNHNLNTSREHHAEIVTTHSYDDRVATIESVKSAGIGTCSGGIIGMGESDDDIIDLA
                     MTLRAMGIDSIPVNFLNSIPKTPFEEKRELTPQRCLKTLCLFRFVNPSKEIRVAGGRE
                     VNLRSLQPLSLYPANSMFVNGYLTTPGQDATDAHQMIRDLGFELDQPAAI"
     gene            complement(5694..6122)
                     /locus_tag="VE170_12475"
     CDS             complement(5694..6122)
                     /locus_tag="VE170_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16301.1"
                     /translation="MPKHGPLLALITFLFACRVLGQSLVAFVGVHWLPAMEQWFSGVI
                     PYPSLLIIQLLLLMLMIRITGEVWRGTGTFAVVRPHWAEFLMKFSGAYAGLMVLRYAL
                     TMILHPEMRWFGDVIPIIFHFVLAGFIFVLGHYHQVMSDC"
     gene            complement(6125..>7319)
                     /locus_tag="VE170_12480"
     CDS             complement(6125..>7319)
                     /locus_tag="VE170_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HYJ16302.1"
                     /translation="SGLIYQSSPRWIQSAFALPYLVADVKDFAAKYYLIMRLALAEPI
                     TFLGTPNPSTILRLVEIADTNKYDIIRDIRDGTISERWQIPGEVRQTLEAQLQKNPAR
                     AAALERLANQHGVLRPQDYWPRLQLIGCWKGGTVGVRLNELHRWFGESMPVRDLGYMA
                     SEAQMTLPISDSGCAGILDISTNFYEFIPENEIASPQPVTLTCTELTAGESYYVILTT
                     ASGLYRYDINDVVRVAGFFQQTPLLEFVRKGRDVTNITGEKLHVNQIIEAMARAQITA
                     NIAPQHFRGFADAAESRYAFMVEFDGTNANPASLKRLLAALEQNLCDLNIEYAQKRAS
                     ERLQPPVLCVMEAGWYARKTSAALRAGGRDTQFKAQLLSATPEQVADITAVIELGENA
                     GRGSD"
BASE COUNT         1413 a         1997 c         2247 g         1662 t
ORIGIN      
        1 acagtcgaaa caggaacgga ttcccgatct attttgcgcc gcactggtgg gcaacttccg
       61 cggttatttc cccggctatt cgaaactgat cgccgagcgg cagaactact tgagttggat
      121 cgttctcaag gcgcatacga tcaaccgcgc cggcacggtt accctgcaat ccgccgatcc
      181 acggcagccg ccgaacatcg acttcaggta tttcgacgaa gggaccgacc gccaggggaa
      241 ggatttagac gcggtcgtgg aaggcattca ttttgtccgg gcgatgacca aggatttgcg
      301 cgaacgaggg ctgatcgccg cggaggaagt gcccggcgaa caatatcaat ccgacgatga
      361 gttgcgagaa ttcgtgcgcg acaatgcctg gggccatcat gcttcgtgca gctgcgcgat
      421 cggtccgcga gaccaggacg gcgtcctgag cagcgatttc cgcgtccatg gtacgcaggg
      481 gctacgtgtg gtcgatgcgt cggtgtttcc gcgaattccc ggctttttta tcgtcagcgc
      541 ggtctacatg atcggcgaga aggccgccga tgtcatcctc gccggcgcca ataattaaat
      601 gcgacttccc tgccggttga attttcttcc cgtccggcga agagaagtgc tgtcataacc
      661 tggcacgcgg gtattcagga atggcggagg gggaagataa agatcaagac acctggattc
      721 ccgtttcacg ggaatgacaa gaagagactg ccgacttcga tcggaagttt tctaatctcg
      781 aaggtcatac gattcgcctg atcgctttca ttgcgcggtc caagacttgt tgcgctcggg
      841 ttggcgagcc tgtatagctc gctctacggc actgatcctg caaaaaacgt tcaccgccgc
      901 tttcgctcta ccacatgagt tcgattcccg ccgagtttta cgccctcgcc aacgctttcc
      961 ttttcgcagt gcacaacatc tttatgaaaa aggcgctgcg ccattcgaac ccggccaccg
     1021 gcgtgatcag cactttgtta atcaatattg tgtttctctg gacgattgcg atcttgtttg
     1081 tgccgatggc caatgtgacc gtggcgtcgc tgctgatttt cgtcggcgtc ggtttctttc
     1141 aacccggtct gacgcgcttg ctcacttaca aaggcatcga tgctttgggt gtggcgatca
     1201 ccgatccgat ccgcgccagc acgccgctgt ttagcgcggc catggcgatc gtttttctcg
     1261 gcgaggagat cactttgccg atcatcgtcg cgaccctcat gatcatcggc ggcattacgc
     1321 tgttgtcttg gcgccgcggg gcgatgaagc tcgccggctc ggcggtctat ctgtggtttc
     1381 ccatcgccgc ctcggtgctt gcgggcgcgt cgcaggtggt gcgcaagttc ggcatggccg
     1441 ccgtgcccca tccgtttctc gccgccgcag tcaccgcgac ttcgtctttg atcatttcgg
     1501 tgctggtgat gtggtacgtc gaaaagactc aagaaacctg gaaaataaat cgccagtgcg
     1561 tctggtggtt tatcgccgcc ggcatcacgg tcagcttcgc catgacctct atttaccacg
     1621 cgttggatct cggcaaagtc tcagtggtga ttcccttgag cagtaccgca ccgctgtttt
     1681 cgttgatcct taccgcgttg ttcttgcgcg acgtcgaacg ggtcacgtta cgaatcgcca
     1741 tcagcacaat gctgatcgtc agcggcgtcg cgctgatcag ttggtggaag tagggaagta
     1801 gaatttagcc gcaatgaacg cagagggcac aaaataaacg gctgatcagg attctaaagt
     1861 tcgtggtgaa accttctttc gcgcgaatct gctcgatcaa tttagacggg acagatagtg
     1921 ggcgaggtct tggaaatcgg cgtcggtgag cggatggagc gcgtcggcca tggcggggtc
     1981 gtaggcgcgc cggctgttgt ttttgtactc gcgcagggat ttgaccaaat attcttcacg
     2041 ttgaccggcg agacgcggcg cattgtcctc gccggcaaaa tctggtttgt gacaaaagtt
     2101 gcagcgatgt tgctggatca ggacgcgagc tttttcggcc cggtcggtat cgggagcgtt
     2161 cgtgctggct ctcggcgccg ggagcttggc gatgatcgcc gcggcgcggc gcagatcgtc
     2221 gtcgctccaa ccttgcatca tggaagtcat gggctcgagg ttgcgcattt tttcccggaa
     2281 catcagcagc tgtacggaaa gaaaaaatac cggttgagcg ccgagggacg gtacctccgg
     2341 gatgaccgac tggccgtttt cgccatggca ggcgaggcaa ggggcgagcc gttcgccgac
     2401 ggccgccgca tttaccgtgg ctgcaacgag taagagataa gctacggtca gagcgcgaac
     2461 gatattggcg ttcactgaga ttggcgaagc taggaccgcc gcgcggtggc ggtgccgcgg
     2521 cggcggttcc ctttgagttc taacgaccgg cgagctgcgg gctgtaggtg acccgataga
     2581 tggcgccggc aaaatcgtcg gagatcaaca tcgagccgtc cttcatgacg tggacgtcga
     2641 ccggacggcc ccagtattcg ttgttgttga gaaagccagt gatgaacggc tgcaccgatt
     2701 ttatcgtgcc gtcgacgttc aacgtcgcga ccacgacatc gccgccgatt ttcttagtgc
     2761 ggttccacga gccatggcgg gcgatgaaga gggcgttacg gtattcgcgg ggaaacatgc
     2821 tgccggtgta gaagcgcaag ccgagggcgg cgctgtgcgg gcccatcaag gccacgggcg
     2881 gggtgaattc gtcgcacgaa cggccccagc ccatctcgcg gtcggtgaag ttgccttggt
     2941 gacagtaggg gaagccgaaa tgctggccga ccttggtgac ccgattcaat tcatcttcgg
     3001 gtaggtcttc cgagagccag tcgcggccat tatcggtgaa gtacaaatcc ttggtgatcg
     3061 gatgccagtc gaagccgacc gagttgcgca cgccgcgggc gaacacttcc gcgccgctgc
     3121 cgtcgagatt gatgcggcgg atctgggcgt tctccgctgg cggttcgcag atattgcagg
     3181 gcgcgccgac gtttatgtag agcttgttgt cggggccgat ggcgataaac ttccaaccat
     3241 gcgactgatg attcggcaag tcggtgtaaa taaccaccgg tttgccggga ttgtcgaggt
     3301 tgtcttcgat tttttccagc ttcgaaatct tggtgccctc gccgatgtag agcgagccat
     3361 tgtgaaaggc gaggccgttg gggcggtcca gccccgaggc gataaccttg gcttcgcgct
     3421 tgccgttgcg ctcgacgacg gcgtatacct tgtcgagttg gcggttgctg acgaacaccg
     3481 tgcccttatc gccgacgcgc agcgacctgg cgttggcgat gccgctgata taaacctcaa
     3541 gcttgaagcc cttgggcatt ttcagttggc tgatctgtaa tttttccgcc ggcgcggcga
     3601 acggcggctg ggccacgccg cgcagcgtct tggccacttc ggcattggga atgtctgggc
     3661 ggccgggcac cattgacggc gccggctgaa cgttgggcgc gggtttttct tgggacagtg
     3721 ccggcgcacc catagacaaa actccgagga tggcggcgac tgcggccgtc cgcgataagg
     3781 tcgggaatga ggtcatggcc tgcctcctct tagcgtttgt acttttgtcg cgagcgaggt
     3841 gcgacaagaa aataggcagg tcgccgcaat attgcaaggc gaaatttcct atccttccgc
     3901 gcggcgggat acggttcgtg ccagtttagg gccgcttggg caggttcttc atcatgtcgt
     3961 tgatattaat attcggcatg cccggcatcg agatgtcgcc cggtttttgg cccggcttgc
     4021 aggcgcccag ccatttggcc tccatcgtcg ctgccgactc tttcatgccg tgcagcggtg
     4081 gttcgtacgt cgatttgatg tcagccttgt agctcgagtc gaattggccg gtaaaaacgc
     4141 ccctggtcgt cgccgtgcta ccgctcatct tacacaccga ttcggtgata agtttggcac
     4201 cttcgcggcg aaactcgttc ttggagcagg attgtttctc ggttccggcc atactgtttt
     4261 tcatcaggtc gtcggacttt tcgtcgacgc attgttgcat ggtctggccg cccttggcgc
     4321 cgtcgctcga cacggttatt tcccataggc cggacttgcg tttgggcatg tcgggagtgg
     4381 cagcgacagc gccacctgca gtagcgacta gcgcgacaat caaagcgagg gttctcatag
     4441 cggtctccta acgtttgctg ccccaccgac aaggtgaatg gatgctagat cgccgccggc
     4501 tggtccagct caaaaccgag atcgcggatc atctggtgcg cgtcggtggc atcttggccg
     4561 ggcgtggtca agtaaccgtt gacgaacatc gagttggcgg ggtagaggct gagcggttgc
     4621 agcgagcgca agtttacttc gcggccgccg gcgacgcgga tctctttgct cggattgaca
     4681 aagcggaata ggcagagcgt cttcaagcag cgttgcggcg tcagttcgcg tttctcctcg
     4741 aagggcgtct tgggaatgga attcaagaaa ttcaccggga tcgagtcgat gcccatggcc
     4801 cgcagggtca tcgccaggtc gatgatatca tcgtcgctct cgcccatgcc gatgatgccg
     4861 ccagagcagg tgccgatgcc ggcgctcttg acgctctcaa tcgtcgccac ccgatcgtca
     4921 tagctgtggg tggtgacgat ctcggcgtgg tgttcgcggc tggtatttag attatgattg
     4981 acccggccga cgccggcggc ggcaagcttc cgggtcttgt cgttgttcat caagccgagg
     5041 gagcagcaga cattcatgcc ggtttgggcg cgaatttcgc gcaccgcttc ggtgatgtcg
     5101 tcgatctctt tgttagtcgg gccgcggccg ctgttaacaa tgcaaaagcg gacggcgccg
     5161 gctgctttgg cctggcgcgc gctttcgacc agcttgtcct tggccatgaa agtgtatttt
     5221 tcgaccgggg cttcggagac cgaggactgc gaacagtaat ggcagtcttc gggacacagg
     5281 ccgctcttgg cgttttgcaa gacatggatc tgcacgcgct tgccgtaata gtgatggcgc
     5341 actttgaaag cagcactcaa gatttccggt agctgttcat cgggtgcgtt caatacttca
     5401 agcagttcct cccgctcggg tagtattcct tgtagagctt tgtccgccag ttgctgaaaa
     5461 ttcatgccgt cctccaaaac tagccagtgt ttagcatgcg aggtgaggcc atgcaatcaa
     5521 accgtcgccc tagggtggta atgattctgt agagttcgcc acttgcgcgc aatcgtcagc
     5581 acacggatta attaccggca aattagtcgt tggctggttg agtgcaacgg tcagttatcg
     5641 accggcgtcc gctggattgg cgcagcgtta ggtgagcggt gtgcgagaaa aattcaacag
     5701 tcgctcatca cctggtggta gtggccaaga acaaatatga agccggccaa cacaaagtga
     5761 aaaatgattg gaatgacgtc gccgaaccag cgcatctcgg gatggagaat catggtcagc
     5821 gcatagcgta ggaccatcaa tccggcgtag gcgccggaaa acttcatcag aaattccgcc
     5881 cagtgggggc gcacgacggc gaaggtgccg gtaccgcgcc agacttcgcc ggtaattctg
     5941 atcatgagca tcaacaggag cagttggatg atcagcagcg agggataggg aatcacgccg
     6001 gagaaccatt gttccatcgc cggtaaccag tgcacgccga caaaagcgac cagcgattgg
     6061 cccaggacgc ggcaggcgaa caaaaatgtt atcagggcta ggagcgggcc gtgcttgggc
     6121 atacctaatc gctcccgcgc cctgcgtttt cgccaagttc gatgacagcc gtgatatccg
     6181 cgacttgttc cggggtggcg ctgagcagct gggctttgaa ctgggtatca cggccgccgg
     6241 cgcgcaacgc cgcgctggtt ttacgtgcgt accagccagc ttccatcaca caaagcaccg
     6301 gcggttgcag ccgctccgat gcccgtttct gggcgtattc gatatttaga tcacacaggt
     6361 tttgctccag ggcggcgagc aatcgcttga gcgatgcagg gttggcgttg gtgccgtcaa
     6421 actcgaccat gaaggcgtag cgagactctg ccgcatcggc gaacccacgg aaatgttgcg
     6481 gcgcgatatt ggctgtgatt tgcgcccgtg ccatcgcctc gatgatttga ttgacatgga
     6541 gcttctcgcc ggtgatgttg gtgacatcgc gccccttgcg gacaaattca agcagcgggg
     6601 tttgctgaaa aaatccagcc acccgcacca cgtcattgat gtcgtaacgg tacaaaccgc
     6661 tagcggttgt caggatcacg tagtagcttt cgcctgcggt caactcggtg caggtaagag
     6721 ttaccggttg cgggctggcg atttcgttct ccggaatgaa ctcgtagaaa ttggtgctga
     6781 tatccaggat accggcgcag ccagaatccg atatcggcaa ggtcatctgc gcctcgctgg
     6841 ccatgtagcc gagatcgcgc actggcatgg actctccgaa ccagcggtgc aattcattca
     6901 agcgcacccc gaccgtgccg cctttccagc aaccgatcaa ttgcagtcgc ggccaataat
     6961 cctgggggcg cagcacgccg tgttggttcg ccagtcgttc gagcgcggcc gcacgggcgg
     7021 gatttttctg tagctgcgct tcgagcgttt gccgcacttc tccgggaatt tgccagcgtt
     7081 cgctgatcgt gccgtcgcga atgtcgcgga taatgtcgta tttattagta tcggcgattt
     7141 cgacgaggcg caggatggtg ctcggattgg gcgtgccgag aaacgttatc ggctcagcca
     7201 acgccaaacg catgatcaga taatatttgg cggcgaagtc tttcacgtcg gcgaccaagt
     7261 agggcagcgc gaaagcactc tggatccagc gtgggctgct ttgataaatc aaaccggag
//
LOCUS       DASQKS010000483         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4047475, whole genome shotgun sequence.
ACCESSION   DASQKS010000483 DASQKS010000000
VERSION     DASQKS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4047475"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1498
                     /locus_tag="VE170_12485"
     CDS             131..1498
                     /locus_tag="VE170_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ16303.1"
                     /translation="MPDTASEQLARQHVALRYEQLPHDVSDAVKLHILDSLGCLLAGS
                     RLEPGRLAYNLSEAGAGNADSTLLGTVRRASYLDAVQAMATAAHCGELDDIHGGAATC
                     IGAMIVPALVALAEKHGGSGRTFLEAAVAGYETTTRIGLSVDAPKLFARGWWPSTVCG
                     AFGVAAAGAKFLGWPVDETANALGIASLHSGGMLTGGQEGATARHLAFGSAARNGLLA
                     LLATEQGLTGPKRALEDPRGFCFTLCAEPKFEFFQDFKQFHLLDVAFKPYPCARQLHA
                     GVEALLRLISRHSQGAGGIREIELAVPTQNAGMIDRPATPTLRAAMLGSGQYVMAVTA
                     LRGKIDLASFDDEYLQSEAVRRLMAHVTVSASTELDRHFPKYWAGRVTVKFADGQAHM
                     EEIIAPKGEAENPMTRLDVEGKFFGLAGPVVGEQKARSVVALVHSLEAADSLAPMLAS
                     LEMSG"
     gene            1630..2412
                     /locus_tag="VE170_12490"
     CDS             1630..2412
                     /locus_tag="VE170_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYJ16304.1"
                     /translation="MSADTTAGYDLLYDIIRRRSSVRKLKPDPIPDAYIGKILEAGRW
                     AMSGANSQPWEYIVVKDAKVKKDLFRCYSEDNTDFIYWMEQQRVFELRHPAYQMTADE
                     AVERQRRGAGWSEAPALIVVVGDGRRQWGTVQGAHTFGRDQSHLTDGLANTCTFMHLA
                     AVSLGLATQWVTIHVQEPFKKVLDVPDLMMLYLIIPIGYPAVQPSEGVRRPLDEIVHH
                     DHYEQDKFMSNERVIQFLYELRGKTLQTYRQSYVGKDEPEKK"
     gene            2419..3456
                     /locus_tag="VE170_12495"
     CDS             2419..3456
                     /locus_tag="VE170_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16305.1"
                     /translation="MGKGSGVLRKFLITLFGLFFVLASNAVSYSAQVSGATKITAGYG
                     GELGYQAPIWLAHELKLFAKHGMTSELVRIAGGARSTAALLANALQVSQSAGVAPVQA
                     NLAGGDLVIIATSTNRPTVSIVGQPKTVKKPQDLVGRTVGLVGPGEMNTFFFLNALER
                     WGIDPKSVTVLAIPGTQPRLAAVVAGTIDATVLAPPFSFEAEKYNLTPLADFATGSDP
                     FPQSGLVVRKELLRSNRDLVKRMLMAYVEAIQILKSDAERSLPIMKKYMRITDEVIAK
                     RSYEYYSKLFSLPPLTDEKAIGVVLKFLATQPGGAGARSAKAEEFFDNSLLQELAKEG
                     FFARLQAGKGS"
BASE COUNT          804 a         1037 c         1212 g          801 t
ORIGIN      
        1 gagctaggag aagattcgga ttcgaaagtt ggccgcaaag aacccaaagg attcacagac
       61 tcgtaatcgt tagccgtagg tggaaaaacc gcggtcctgg tttatcgcgc gaatacaagt
      121 aataccgacg atgcccgaca ctgcaagcga gcaactcgcg cgtcaacatg tggcgctacg
      181 ctacgaacaa ttgccgcacg atgttagcga cgccgttaag ctccacatcc tcgattccct
      241 cggttgtctt ctcgccggct cgcgcttgga gccgggacgg ttggcctaca atctgtctga
      301 ggcaggcgcc ggcaacgccg attcgacgct tttaggaacg gttcgacgcg cttcgtatct
      361 cgatgccgtg caggcgatgg cgacggcggc gcattgcggc gaactcgacg atatccatgg
      421 cggcgccgcg acctgcatcg gcgccatgat cgttcccgcc ctagtggcgc tggcggaaaa
      481 gcatggcggc agcggtcgga cttttctcga agcggcggtc gccggctacg aaaccaccac
      541 gcgtatcggt ttgagcgtcg atgcgccgaa gctgtttgcc cggggctggt ggccgagcac
      601 ggtttgcggc gccttcggcg tggccgccgc gggggcgaaa ttcttgggct ggccagtcga
      661 cgagacggcc aacgccctcg gcatcgcttc gcttcacagc ggcggcatgc tcaccggcgg
      721 acaggaaggc gcgacggcgc gtcatctcgc tttcggttcc gcggcgcgaa atggcctgct
      781 cgcactgctc gccaccgagc agggattgac agggccgaag cgcgcgttgg aagatccgcg
      841 cggtttttgt ttcacgcttt gtgccgaacc gaaattcgag tttttccaag acttcaaaca
      901 gtttcatttg ctcgacgtcg cgttcaaacc ctatccctgc gcgcgccaat tgcatgccgg
      961 ggttgaagcg ctgctgcgat tgattagccg gcattcgcag ggcgccggcg ggattcgcga
     1021 gatcgaactg gcggtgccga cgcaaaacgc cggcatgatt gatcgtccgg cgacgccgac
     1081 gctgcgcgcg gcgatgttgg ggagcgggca atatgtgatg gcggtaacgg cgctcagagg
     1141 caaaatcgat ctggcgtcat tcgacgacga gtatttgcag agcgaggcgg tgcgtcgatt
     1201 gatggcgcac gtgaccgtca gcgccagcac ggagcttgat cggcatttcc ctaaatactg
     1261 ggcagggcgg gtaacggtga aatttgccga cggtcaggcg catatggagg aaatcattgc
     1321 gccgaagggc gaggccgaga atccgatgac gcgtttggac gttgaaggaa agttttttgg
     1381 cttggccggg ccggttgtgg gcgagcaaaa ggcacgatcg gttgttgcat tggttcattc
     1441 tctcgaagcg gctgattcac tggcgcctat gctagcgtcg cttgagatgt cagggtagcg
     1501 gcgtcgcgca gccgagcttt tccacaggcg gccggggcgc aggacataag gtcgtttgaa
     1561 gcggcgcgca accctgagct ataactatcg agcttcaagc taatcgtact cagatttgga
     1621 ggatccttta tgagcgcaga taccaccgca ggttacgatc ttctctatga catcatccgg
     1681 cgccgctcga gcgtgcgcaa gttgaagccc gatccgatac ccgacgctta tatcggcaag
     1741 atactcgagg cagggcgctg ggcgatgtcg ggggcgaact cgcagccttg ggaatacatc
     1801 gtcgtcaaag atgccaaagt gaagaaagat ttgtttcgct gctactcgga ggacaacacc
     1861 gattttatct actggatgga gcagcagcgg gttttcgagc tgcgccatcc ggcctatcaa
     1921 atgaccgccg acgaggcggt tgaacggcag cgccggggcg ccggatggtc ggaggcgccg
     1981 gcgttgatcg tcgtcgtcgg cgatgggcgg cgccagtggg ggacggtgca gggcgcgcat
     2041 acgtttggcc gcgatcaatc gcatcttacc gacggcttgg ccaacacttg cacgttcatg
     2101 catctggcgg cggtgtccct cgggctcgcg acccaatggg tgacgattca cgtccaggaa
     2161 ccgttcaaga aagtactcga tgtgcccgat ctgatgatgc tctatctgat tattcccatc
     2221 ggttatcccg ccgtgcagcc gtccgaaggc gtgcgccggc cgctcgacga gatcgtgcat
     2281 cacgatcatt acgaacaaga caaattcatg agcaacgaac gggtgatcca gtttctttac
     2341 gagctgcgcg gaaagacatt gcagacctat cggcagtcgt atgtcggcaa ggatgagccg
     2401 gaaaagaagt aacggatgat gggcaagggg tcaggcgtgt tgagaaaatt tttaataaca
     2461 ctcttcggct tatttttcgt tttagcctct aacgcggttt cctatagcgc acaagtcagc
     2521 ggcgctacga aaatcaccgc gggttatggc ggcgagttgg gttatcaggc gccgatatgg
     2581 ctggcccatg agctgaagtt gttcgccaag catggcatga cctcggaatt ggtgcgcatc
     2641 gccggcggcg cgcgcagcac ggcggcgttg ttggccaatg ccctgcaagt gtcgcagagc
     2701 gccggcgtgg cgccggtaca agcgaatttg gctggcggcg atctggtgat catcgcgact
     2761 tcgaccaatc ggccgacggt gagtatcgtc ggccagccga agacggtgaa gaaaccccag
     2821 gatctggtcg gcaggaccgt tggtctggtc ggtccggggg agatgaacac tttctttttt
     2881 ctcaatgcgc tggaacgttg gggcatcgac ccgaaatcgg tcactgtcct ggcgattccc
     2941 gggacacagc cgcggctcgc cgcggtggtc gccggcacca tcgatgcgac ggtgttggcg
     3001 ccgccgttta gcttcgaagc ggaaaagtac aatctgacgc cgctggcgga cttcgccacc
     3061 ggatcggatc catttcccca atccggtttg gtggtacgca aggaactgct gcgcagcaac
     3121 cgcgatctgg tcaagcgcat gctgatggcc tatgtcgaag cgattcagat actcaaaagc
     3181 gacgccgaga gaagcctgcc gatcatgaaa aagtacatgc gcatcaccga cgaggttatc
     3241 gccaagcgca gttacgagta ctattccaag ctgttttctc taccgccgtt gaccgacgag
     3301 aaagcaatcg gcgtggtgct taagtttctc gccacccagc caggcggggc cggtgccagg
     3361 agtgccaaag cagaagagtt cttcgacaat agtttattac aggagttggc caaggaaggt
     3421 ttcttcgcgc gcctgcaggc tggcaaaggg tcgtgaggcg acagaagagc tttaaccaaa
     3481 atgagcacct aggcgacaaa gtaagaagaa tcggaatcga tcgtttaacc gcaaacggcg
     3541 ctatgagcaa ccggccggtc gttcttgtaa ttggaagtct catttgtgcg ctttacgttc
     3601 tctgcggcta catcgccgat tgggaaattc tatatatgtg aactttgtgc tctttgtggt
     3661 taaaattccg aggtgtctat gtccgtcgat cccatcactg tagaaattat tcgctgcgct
     3721 ttgaaggccg ccgccaacga gatgtcggcg gtgctgaaga agaccgctta caacatgatg
     3781 atctacgagg tgcaggacta ctgcgtcgcg gtgctcgatc acgaaggccg gacgatgtcg
     3841 caaaacgagg gggc
//
LOCUS       DASQKS010000484         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1111151, whole genome shotgun sequence.
ACCESSION   DASQKS010000484 DASQKS010000000
VERSION     DASQKS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111151"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VE170_12500"
     CDS             <1..260
                     /locus_tag="VE170_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HYJ16306.1"
                     /translation="RVAIARAFMNQPKILFADEPTGNLDAETAESIVKLIFNLNSMNR
                     TTLILVTHDRDLTDQADRVITLKAGRLASDITQSATAAATA"
     gene            270..2807
                     /locus_tag="VE170_12505"
     CDS             270..2807
                     /locus_tag="VE170_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYJ16307.1"
                     /translation="MITFWKLWVWRMAYRDSRRGLLRLLLSMSCVALAVALVVLAFSF
                     RENLLASIQSQSKSLLGADLAIDSREPFSKDDLQLIHSLGGDQSRQVGFTSMVTFSAN
                     GDSRLVQVRAISGQFPYYGALETVPQLSLDAFHLSANALVDENVMLQFNAKVGDFVKI
                     GSQEFRIAGGLRKIPGETLAFSLISPRIYIPMAYLDRTRLIQTGSVVRYRVFFKFAPG
                     TQVDRLVKEITPQLQRLQLEADTVSRRTASIAANMENLSRFLRLAVFVAVLLAGVGVG
                     SVTYVYAKEKAQAAALLRCIGARPRETVSVYLLQLFFLTLVCSAAGVGLGVWAQSYLP
                     ALLKDFLPVAPTIEISPLGIAAGVGIGMTTSFLFALLPLISLRRISPLMALRASYDDE
                     PRTKDPLVLFIVFLIALLVLAFASATLGSWRQGAIFAGGVLVVFVLLALLARMGALAM
                     RQVAPRFLTFSWRQGLANLHRPSNQTTTVMLAIGLGTFLLVTLHGAQDTLLRQVDRRI
                     GHNEPNLVLFDVQNEQRKAIGELLSRFEIPLHAEVPIVTMRLAAVKGRRAEAIRADEK
                     SKVPGWALRREYRSTYRGILTGTEKLVKGNWVAKADEQLQPIPVSVEKGLADTLGVDV
                     GDSLEFEIQGVPVNTIIANLREVDWQRVQPNFFVVFPEGVLEQAPQFFAVVGRAPTTQ
                     ASAQLQRAVLERFPNVSAIDLSLILSTLNTVLDRVANAMRFVAWFTLLTGFAVLASAV
                     LGSRGHRVRESILLRTLGAARGQIFNSIVAEYVFTGAIASIAGAILGILASWGLSYYF
                     FGTAISIAPLPVLVIILAVTAATVIAGALGCLGVIRRPALEALRAEA"
BASE COUNT          522 a          874 c          864 g          728 t
ORIGIN      
        1 agcgggtggc gatcgcccgg gcgttcatga atcagccgaa gatattgttt gccgacgaac
       61 cgaccggcaa tctcgacgcc gagaccgccg agagtatcgt caagctgatt tttaacttga
      121 actcgatgaa tcgcaccacg ctcattctcg tcacccatga tcgggacctc accgatcagg
      181 cggatcgggt catcacgcta aaagccggtc gtttggcatc cgatattact caatccgcga
      241 ctgccgctgc caccgcctaa gccggttcga tgatcacctt ctggaaactc tgggtctggc
      301 gcatggccta tcgcgacagc cggcgcggtt tattgcgctt gttgctgtcg atgtcctgcg
      361 tggcgttggc ggtggccttg gtagtgttgg cgttttcctt tcgagaaaac ctcctagcca
      421 gcatacagtc tcagtctaaa agtctcttgg gagccgatct cgccatcgac agccgcgaac
      481 cgttttccaa ggacgattta caactgattc attccctggg tggcgatcag tcgcggcagg
      541 tcggttttac ctcgatggtg actttctccg ccaacggcga ctcgcgtctg gtgcaggtgc
      601 gcgcgattag cggccaattt ccctactacg gcgccttgga aactgttcca cagttatccc
      661 tcgacgcgtt tcatttgagc gccaacgcct tggtcgatga gaacgtgatg ttgcagttca
      721 atgccaaggt cggcgatttc gtcaagatcg gcagtcaaga gtttcgcatc gccggcggct
      781 tgcgcaaaat tcccggtgaa acgctcgcat tttcactgat cagtccacga atctatattc
      841 ccatggcgta tctggaccgc acccggctga tccaaactgg cagcgtggtg cgctatcgcg
      901 tgttttttaa attcgcccct ggaacgcagg tcgaccggct ggtgaaggag atcacgccgc
      961 agttgcaacg cttgcagttg gaagccgata cggtcagcag acgcaccgct tcgatcgccg
     1021 caaatatgga gaatctgtcg cgctttctgc gcttggcggt atttgtcgcc gtgctgctcg
     1081 ccggcgtcgg cgtcggcagc gtcacctatg tttacgccaa agaaaaagct caagccgcgg
     1141 cgctgttgcg ttgtatcggt gcccggccgc gggaaaccgt ctcggtttat ttattacagc
     1201 tgtttttcct gaccttggtt tgctcggcag cgggagtggg gttgggtgtg tgggcgcagt
     1261 cctatttgcc tgcgttgttg aaagattttc tcccggtggc gccaaccata gagatttcgc
     1321 cactcggtat cgctgccggt gtcggcatcg gcatgacgac gtctttcttg tttgcccttt
     1381 taccgttgat ctcattgcgg cggatttcgc cgctgatggc gttgcgtgct tcctacgatg
     1441 acgaacctcg caccaaggat ccgctggtcc tattcatcgt attcttgatc gccctgttgg
     1501 ttctggcttt cgctagcgca accctcggta gctggcgtca aggggcgata tttgccggcg
     1561 gcgtgctcgt agtattcgtc ttgctcgcgc tgttggcgcg catgggcgcg ctcgccatgc
     1621 gtcaggtggc gccgcgcttt ctgaccttct cctggcgcca gggtttggct aatctccatc
     1681 ggccgagcaa tcagaccacc acggtgatgt tggccatcgg cctcgggaca ttccttttag
     1741 tcacgctgca tggcgcccag gacaccttgc tgcgtcaggt cgaccgccgc atcggccata
     1801 acgagcccaa tttagtgctc ttcgatgtgc aaaatgagca gcgcaaagcc atcggggaat
     1861 tgttatcccg tttcgaaatc cctctccatg ccgaagtgcc aatcgtcacc atgcgtctgg
     1921 cggcggtcaa ggggcgccgc gccgaagcga tccgcgccga tgagaaatcg aaggttcccg
     1981 gctgggcgct gcgtcgcgag taccgttcga cttatcgcgg gattctgacc gggacggaaa
     2041 aactggtcaa aggcaactgg gttgccaagg ccgatgaaca actgcaaccg attccagtct
     2101 ccgtcgaaaa gggtctcgcc gatacgctgg gcgtcgatgt cggcgactcg ctggaatttg
     2161 aaatccaggg cgtgcccgtc aatacgatca tcgccaatct gcgcgaagtc gactggcagc
     2221 gggtacagcc gaattttttc gtggtctttc ccgaaggggt cttggaacaa gcgccgcagt
     2281 ttttcgccgt ggtcggacgc gcgccgacga cccaagcttc ggcccagttg cagcgcgcgg
     2341 tgctggagcg cttccccaat gtctcggcga tcgatctgag tctgattctc agcacactga
     2401 acacggtcct cgaccgggtc gccaacgcca tgcgcttcgt cgcctggttt accttgctca
     2461 ccgggtttgc cgtgttggcc agcgctgtgt tgggcagccg cggccatcgg gtgcgcgaga
     2521 gcatcctgct gcgcaccctc ggcgcggcac gcgggcagat ctttaactcg attgtcgcgg
     2581 aatatgtttt caccggcgcc atcgccagca tcgccggcgc gattctcggc atcctggcga
     2641 gttgggggct cagttactat tttttcggca ccgccatttc catcgcgccg ttgccggtgc
     2701 tggtgattat tctcgccgtg accgctgcga cggtaatcgc cggtgcgctt ggctgcctcg
     2761 gcgtgatccg ccgcccggcg ctggaagcgc tgcgcgccga ggcttaacgg cgttcgcaac
     2821 tgtcccttgt catttcgagc gcagccagaa aaatcttctc catcttgtgc atagttggat
     2881 ctaagtcgcc gtgatcattg acgcatggtc ggttcgtggc ggcatcggtg atttcctgat
     2941 aatttgcggc ttcgttcatc aaaaatcatt caaatgaatt tattaacc
//
LOCUS       DASQKS010000485         5544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3642648, whole genome shotgun sequence.
ACCESSION   DASQKS010000485 DASQKS010000000
VERSION     DASQKS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3642648"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VE170_12510"
     CDS             <1..188
                     /locus_tag="VE170_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16308.1"
                     /translation="NFSLLTFKPIVVERYHRKKRKLFKKFPCGTDGRFPIAHYHERVT
                     STLNSPQALGVGTAATT"
     gene            complement(411..4823)
                     /locus_tag="VE170_12515"
     CDS             complement(411..4823)
                     /locus_tag="VE170_12515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ16309.1"
                     /translation="MNELFHPLVERWFTERFHQPTPAQAGGWASIAAGKATLIAAPTG
                     SGKTLAAFLWSIDRLIKQALGGALQERTSVVYVSPLKALGNDIAKNLQAPLAEIYQLA
                     GREGILLPSIRVAVRSGDTPARERQAMVRRPPHILITTPESLYILLTSARSREFLRSA
                     ETLILDEIHAIAQDKRGAHLALSIERLAALAERPLQRIGLSATQKPIDEVARLLVGTR
                     QIGDDGAPDCSIVDVGHQREMELRIEVPDEELGPIIRQDIWAAVYDKLVEQINAHRTT
                     LIFVNTRRLVERVAHQLSERLGEDKVGAHHGSLSRKTRLAVEEKLKSGQYPVVVATAS
                     LELGIDIGHVDVVCHIGSPRNLAVLLQRVGRSGHWLGAIPKGLFYPLTRDDLMQSVAA
                     IRAVRQGQLDKLNLIHQPLDILAQQMVATVASSRLAAKHAKGANDEEGISEEALWLLM
                     RAAYPYRDLQREEFEQVLEMLSEGVETRRSRRSAYIHRDRINGMLRPRRGARLTAITN
                     GGAIPDTADYHVVEFASETFVGTVHEDFAIESLAGDIFLLGNRSWRIRRVGSGKVWVD
                     DAQGLPPSIPFWMGEAPARTLELSQAVADLRVAVADRIGDRVAAAAWLQAEVSMAQAA
                     AEQLVNYVADTIAVLGTVPSQDKIIAERFFDEAGGMQLILHAPWGGRINRAWGLALRK
                     RFCVSFDRELQAAATDDGICISLVEQHSFPLSDVFAMLKPAILEYNLTQAALASPMFA
                     NRWRWNATRALALRRSNGGKKIPVAIQRMRAEDLLAAVFPEQVMCQDNRGGGIELPDH
                     PLVKETMGDCLHEAMDIDGLAALLGKIDSGEIATLAIDTPAPSPMAHEILNANPYAFL
                     DDAPLEERRARAVSLRRVDPRLDREFGQLDPQAIAEVCKQAWPEARNADELHDLLLGV
                     CLLPADLRQDWQSLAGALIDHGRATVATWQASNGGQRAIVAAERVDLIKLCVPEVSFS
                     PAIALPLGFAETVATAEDAIKKIVQGWLEVSGPVSAAALAIRIGLPVNKVEAALLALE
                     SAGVVMRGQFTGSMRGAQGAPNAQNTNNGQDTEAVEWCDRILLARIHRLTLGKMRREI
                     EPVSAAEFINFIIHWQHAAPGSRLRGRDGVLRVIEQLQGLELPAPAWEQHVLPARIEN
                     YDPADLEYLCLSGIVAWGRLRNDTLPEEPGLPKPPSGKRLPRLLAPARNAPIGFILRD
                     ELENYLEPIHGRYTDIPSLSTMALDVARYLEQQGASFLADIARDTGLLKVKVEEALWQ
                     LVAHGLASGDGIAGLRVLLTPEHKRTERRRSLRLISGGRSSERAMPVGRWSLWRAKFS
                     QLEVSSTSRMERQARQLLQRYGVVFRDLLARESSTGSWGTWRNLLAIYRRLEARGEIR
                     GGRFVNGFVGEQFALPEAVERLRELRRSKAVTSEILLAAADPLNFAGILTPGPRLSPY
                     SNQCLAYRNGEMIDSGLLGELMSRLQSPESSHRT"
     gene            complement(4945..>5544)
                     /locus_tag="VE170_12520"
     CDS             complement(4945..>5544)
                     /locus_tag="VE170_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16310.1"
                     /translation="LYPALFGMIGGVLKSLCIAVGWILYPQGRDISTIIAGLYLSQGL
                     FFMSICMTKGATEHSTSLLRTGALIGLLSGILATSAIVLFLYRGADLTAFDHAVVNGS
                     FLGGAVGIVYGVGSTDRRWVYLIVGFLGGGIGVLSLHSSGTIADLTAMLLWKLSSSLD
                     IAMVYGKWIVYPLDGITQSLLIWFSLAYAKEFFGVRDRN"
BASE COUNT         1191 a         1685 c         1580 g         1088 t
ORIGIN      
        1 ataatttttc cttgttaacg ttcaaaccta tcgtggttga gcgatatcat cgcaagaagc
       61 gcaagttgtt caagaagttt ccttgcggta ccgacggacg ttttcccatc gcacactatc
      121 atgagagagt tacctcaacc ctaaattcac cgcaagcgct tggcgtaggc accgcagcga
      181 ccacataatg ccagatgtcg gatgtggatc gccttgatgc cgaactcgaa tccgctaatt
      241 gcgccaaccc ggcgtctagc acctgtcgtc atgaacgcga agactcatca cacctggaaa
      301 ccatcgcctt ggcgaatcgg agtcgtcgct cgcggaaatt cactgcccag ctaggtttga
      361 catgaagatt ttatgtggat tggggaatta aagaagttaa acgtaaacga tcaggttcga
      421 tggctgcttt ccggcgattg caaacggctc atcagttcgc ctagaagtcc cgaatcgatc
      481 atttcgccgt tgcggtaggc gaggcactga ttggaatacg gcgagaggcg cggtcccgga
      541 gtcagtatcc cggcaaaatt caatggatcg gcggcggcca gtaaaatttc gctggtcacc
      601 gctttgcttc ggcgcaactc acgcaagcgc tccaccgctt ccggcaaagc aaattgctcg
      661 ccgacaaaac cattgacgaa acggccgccg cggatctcac cgcgcgcctc caagcgccga
      721 taaatcgcca acaaattacg ccaggtgccc cagctgccag tgctggattc gcgcgccaat
      781 aagtcgcgaa acaccacgcc gtagcgctgt aatagctggc gcgcctggcg ttccatgcgg
      841 ctcgtgctgc tgacttccag ttgcgagaat ttcgcgcgcc acagcgacca gcgtcctacc
      901 ggcatggccc gctcggaact gcgcccgccg ctaatcaagc gcaagctgcg gcgccgttcg
      961 gtacgcttgt gttcgggagt caggagtacg cgtaagccgg cgatgccgtc gccgctagcc
     1021 aacccgtgag cgaccagttg ccaaagtgcc tcttcgactt tcactttgag caggccggtg
     1081 tctctggcga tatcggcgag aaaggaggcc ccctgttgct caagatagcg cgcgacatct
     1141 agcgccatgg tcgacaggct cgggatatcg gtatagcggc cgtgtatcgg ttcgagataa
     1201 ttttccagct cgtcacgcag gatgaaaccg atgggcgcat tgcgcgccgg cgccagcagt
     1261 cgtggcaatc tctttcccga aggcggcttc ggcaggccgg gctcttcggg cagggtatcg
     1321 ttgcgcagcc tgccccacgc gacgatgccg ctcagacaga gatactccag atcggcgggg
     1381 tcgtagtttt cgatgcgtgc cggcagcaca tgctgctccc aagccggcgc gggcaattcc
     1441 aagccctgta actgttcgat gacccgcagc acaccgtccc gcccgcgcaa tctgctaccc
     1501 ggggcggcat gttgccagtg aatgataaaa ttgatgaatt cggccgccga taccggttca
     1561 atctcacggc gcatcttgcc cagagtcaat cgatggatgc gcgcgagcag tatccgatcg
     1621 caccactcga cggcttcggt atcttggcca ttattagtat tttgggcatt tggggcacct
     1681 tgggcgccgc gcatactccc agtgaattgg ccgcgcatca ctaccccagc gctttccaag
     1741 gcaaggagtg cagcctcgac tttgttgacc ggcaggccga tgcgtatggc gagcgccgcg
     1801 gcgctgaccg gcccgctgac ttcgagccag ccttgaacga ttttcttgat agcgtcttcc
     1861 gccgtcgcca cggtctcggc aaaaccgaga ggtagtgcga tcgctgggga gaacgaaacc
     1921 tccggtacac agagtttgat caaatcgact cgctcggcgg cgacgatggc gcgctgcccg
     1981 ccgtttgagg cctgccatgt cgcgaccgtc gcacggccgt ggtcgatcaa ggcgccggca
     2041 agcgactgcc aatcctgccg caggtccgcc ggcagtaggc atacgcccag aagtaaatca
     2101 tgcaactcat cggcgttgcg cgcctcgggc cacgcttgtt tgcagacctc ggcgatggct
     2161 tggggatcga gctggccaaa ctcccggtcc aaccgcggat cgacccgccg cagcgacacc
     2221 gcccgcgccc ggcgctcctc caatggcgcg tcgtcgagga atgcataggg attggcgttc
     2281 aagatttcat gagccatcgg cgacggtgcc ggtgtgtcga tggcgagagt ggcgatctcg
     2341 ccgctgtcga ttttgccaag aagcgccgcc agaccatcga tatccatcgc ctcgtgcaga
     2401 cagtcaccca tggtctcttt gaccagtgga tgatccggga gctctatccc gccgccgcga
     2461 ttgtcttgac acatcacctg ttcgggaaac accgccgcga gcagatcttc ggcgcgcatg
     2521 cgctggatcg ccacggggat ctttttgccg ccgttactgc gcctgagcgc cagggcccgg
     2581 gttgcgttcc aacgccaacg gttggcgaac atcggcgacg ccagcgcggc ctgagtaaga
     2641 ttgtattcga ggatcgccgg cttgagcatg gcgaacacgt cactgagagg aaaggagtgc
     2701 tgttcaacca gcgagataca gatgccgtca tcggtggccg ccgcttgcag ttcgcggtcg
     2761 aagctgacgc aaaaacgctt gcgcaacgcc aagccccaag cccggttgat ccgcccgccc
     2821 caaggcgcgt ggagaatcaa ttgcatgcca ccggcctcgt caaaaaagcg ctcggcgatg
     2881 atcttgtcct gcgacggcac ggtgccgagt acggcgatgg tatcggcgac gtagttgacg
     2941 agctgctcgg ccgccgcttg tgccatcgac acttcggctt gaagccaggc cgccgcggcg
     3001 acgcggtcgc cgattctgtc tgccaccgcc acgcgcaaat cggcgaccgc ctgggataac
     3061 tccagtgtgc gcgccggcgc ctcgcccatc cagaatggaa tactcggcgg taatccttgg
     3121 gcgtcgtcaa cccagacttt gccggaaccg acgcggcgaa tgcgccagga gcgattgccc
     3181 aagagaaata tgtcgccggc caaactctcg atggcaaaat cttcgtgcac cgtgccgaca
     3241 aaggtttcac tggcaaattc gacaacatgg taatccgcgg tatcgggaat cgctccaccg
     3301 ttggtgatcg ccgtcaagcg cgcgccgcgc cggggccgca gcatgccgtt aatccgatcg
     3361 cgatgaatgt aagcgctgcg tcggctacgc cgggtctcga cgccttcgct gagcatttcc
     3421 aaaacctgct cgaactcttc ccgctgcaaa tcacggtagg gataggcggc gcgcatgagt
     3481 agccacagcg cttcttcgga aatcccctct tcgtcatttg cgccctttgc gtgctttgcg
     3541 gccaatctgg aactcgccac cgtcgccacc atctgctgcg ccaagatatc caacggctgg
     3601 tgaatcaagt taagcttgtc caactgcccc tgacgcacgg cgcgaatcgc cgccaccgac
     3661 tgcatgagat cgtcgcgcgt cagcgggtag aaaagcccct tgggaatggc gccgagccag
     3721 tggccggagc ggccaacccg ttgtagcaat accgccagat tgcgcggcga gccgatatgg
     3781 cagaccacgt cgacatggcc gatatcgatg ccgagttcga gcgaagccgt ggcgaccacc
     3841 accggatatt gaccggattt gagtttttcc tcgaccgcca agcgggtttt gcgcgacagg
     3901 ctgccatgat gcgcgccgac tttgtcttcg cccaaccgtt cgctcagctg gtgtgcgacc
     3961 cgctcgacca ggcggcgggt gttgacgaaa atcaaagtcg tgcggtgcgc gttaatttgc
     4021 tcgaccagct tgtcgtaaac cgccgcccag atatcctggc gaatgatcgg tcccaactct
     4081 tcatccggca cttcgatacg cagttccatc tcgcgctgat ggccgacatc gacaatcgaa
     4141 caatcaggcg cgccatcgtc gccaatctgt cgcgttccga ccaaaaggcg cgctacttca
     4201 tcgatgggtt tctgcgtcgc gctcaaaccg atgcgctgca gcggccgctc ggccaaggcc
     4261 gccagccgtt caattgaaag tgctagatgg gcgccccgtt tgtcctgggc gatggcgtga
     4321 atttcgtcga ggatcaaagt ctcggcggag cggagaaatt cccggctgcg cgccgaggtc
     4381 agcagaatat acagagactc cggtgtggta ataagaatat gcggcggccg gcgcaccatc
     4441 gcctggcgct cccgcgccgg cgtatcgccc gagcgcaccg ccacgcggat tgacggcaaa
     4501 agaatcccct cgcgtcccgc caattgataa atctccgcca gcggcgcctg gaggttcttg
     4561 gcgatatcgt tgccgagggc ttttaagggc gaaacgtaga cgacgctggt gcgttcctga
     4621 agtgcaccgc cgagcgcctg cttgatcagc ctgtcgatgg accaaagaaa cgccgcgagc
     4681 gtcttaccag acccggtggg cgcggcaatc aaggtcgcct tgcccgcggc aatagacgcc
     4741 caacctccgg cttgcgccgg cgtcggctgg tgaaatcgtt ccgtaaacca gcgctcgact
     4801 aagggatgaa ataattcgtt caagctagat ttctccgcgc gtggagaatt tcaagctaaa
     4861 gggaattaag gcgagggtca ataagaagcg gcaaattgtc agtcatgcgg caatagatgc
     4921 gggggtttcg cgtagggtga caccctaatt cctatcccgc acgccaaaaa attctttcgc
     4981 gtaagccaga ctaaaccaaa taagaagtga ttgggtgatt ccgtctaagg gataaacaat
     5041 ccactttccg taaaccatgg cgatgtcgag cgatgaggaa agcttccata gtagcattgc
     5101 cgtcaggtct gctatcgtgc ccgacgagtg aagggataaa accccaattc cgccgcccaa
     5161 aaaccccacg ataagataaa cccaccgcct gtcggtgctg cccaccccgt aaacgatacc
     5221 aacagctcca cctaagaatg acccgttgac gaccgcatga tcgaacgcag ttagatcagc
     5281 cccgcgatat agaaataaaa caatcgcgct ggttgctaaa attcctgaaa gaagtccgat
     5341 caaagctcct gtcctaagaa gtgatgttga atgctctgtg gcgcctttcg tcatacaaat
     5401 cgacatgaag aatagaccct gtgataaata caaacccgca ataatggtgg aaatatcgcg
     5461 cccttgcgga tagagaatcc agccgactgc aatgcacaag ctcttcaata ctccgccaat
     5521 cattccaaat agagcggggt agag
//
LOCUS       DASQKS010000486         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3212296, whole genome shotgun sequence.
ACCESSION   DASQKS010000486 DASQKS010000000
VERSION     DASQKS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212296"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VE170_12525"
     CDS             <1..333
                     /locus_tag="VE170_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16311.1"
                     /translation="AVIDHPFSPFLNVGPDMMKEIRGTGVLFNFTWDELSPLLGVDPQ
                     IMYNTIREVGVEHFTLSSDAGEPLFPDSVEAMRLVRGYMEAFGLNQDELYTVCTKNPA
                     KVVGLALQ"
     gene            complement(423..1418)
                     /locus_tag="VE170_12530"
     CDS             complement(423..1418)
                     /locus_tag="VE170_12530"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16312.1"
                     /translation="MITQVVRRCGSLVIAYFWISCPAFAAERIVGLQSAPSVAMALPW
                     FAEEARLYAKHDLDFQLVYIASSGIVTAAMSGGSGNVAIVGGEGPIRAYISGNTDFVF
                     IGSVKNVLTHSLMGKADLKRPEDLKGKRIGVGRIGGNAHYFTMYGMRQRGLDPLKDAN
                     LIQTGGAPETFLALSTGAVDAASLTTPQDTRAAFLGFNYVIDGREIKPAYVATGFVTL
                     RSVIAKRPKAISQFMHVMAESLKIMISDRELAFKVISRKIGLTDRKVFDAAYTAELKV
                     LEPRLDIKAGAIQANLDEIARTDPRAKQVKAEQLIDRRFLDEMEKDGTFDKLGLK"
     gene            complement(1480..2466)
                     /locus_tag="VE170_12535"
     CDS             complement(1480..2466)
                     /locus_tag="VE170_12535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16313.1"
                     /translation="MSIMVRTLLALGILILNVQLSAAQEKVRVGWAAMTASHTPLWVA
                     QERGLFAKQGLVSEPIFFGAGPPAMQALVAGDLDIVITSAPNVVNPRLGGADFVMILS
                     IIPTFIDHIISAGNITTVEQLRGKTGSVNRAGSISDMGMKLSLKRLGIDPDKDVKIVP
                     AGGNPERLASISRGLTHFTIMNEPFIKEAERLGFRDLVNMATLKIPLHGNGVVVRETT
                     IKARRPMVSRFARAITEALHLVKADKEGTKAIIGKYTRLTDPEGLERTYKNYTSVLLD
                     VPYADPAGIKTLLDDMAAKNPKAAAADPKSFVDASFVQEMEASGFIKQLSKR"
     gene            complement(2586..3572)
                     /locus_tag="VE170_12540"
     CDS             complement(2586..3572)
                     /locus_tag="VE170_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16314.1"
                     /translation="MAAIIKAMLGLSILFLNVQISVAQEKVRVGWAAVSAAHTPLWVA
                     LEKGLFAKQGLSPEAIFFGAGPPAMQALVAGDLDMVVTSAPNVVNPRLGGADVVMLLS
                     IVPTFTDHIISAATITSVEQLKGKSGSVNRAGSITDMGLRLALKRFGIDPEKDAKIVS
                     VGGNPERLASIARGITQFTILDEPFIKEAERLGFRDLVNLATLKISLHINGVVTREAT
                     IKSRRPMVSRFARAMTEAIHLVKADKEAAKAAIAKYTRLSDPDGLERTYRNYSAVLLD
                     VPYADPVGIKTLLDDMAPKNPKAAGADPKSFVDASFVHEMESSGFIKQLSKR"
     gene            complement(3733..>4690)
                     /locus_tag="VE170_12545"
     CDS             complement(3733..>4690)
                     /locus_tag="VE170_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16315.1"
                     /translation="PADPAGDAAGAVEKSEIVVAYPQPSGVFTPLFVASDGGLFKKYG
                     LNVKLQQLNPQLSVQSVVSGSADMSVAAGDLVNASLQGAKIKLVGSSMSQLVFQLWTA
                     KEIASVQQLKGKTLAGTTPRSVLEIATREALKRQGLSFNSDYKLIYMQSVPAVLTAIV
                     SGKAAGGALSAPTTLKAKDAGLNMLVDIAKANIPGLPLAYGFTEQFIKQNPNTISAFL
                     KGIAEGVARTKNDPAAAKRAIAKFTQTEDVKAIDDTYDFYAPYFVTHLALRPEQLTTW
                     FSYLDETEYPAAAKANPRDFYDNSFVEALEKTGFFQKLGAGK"
BASE COUNT          976 a         1356 c         1304 g         1054 t
ORIGIN      
        1 gcggtcatcg accatccgtt cagcccgttt ttgaatgttg gaccggacat gatgaaagaa
       61 atccgcggca ccggagtgct atttaacttc acctgggacg aactctcacc gctcttgggc
      121 gtggacccgc aaatcatgta caacacgatt cgcgaagtcg gtgtcgagca tttcacgctt
      181 tccagcgacg ccggcgaacc gctttttccc gactccgtcg aagccatgcg cctggtgcgt
      241 ggctacatgg aagccttcgg tttaaaccag gacgaacttt atacagtgtg cacgaagaac
      301 cccgccaagg tggtaggcct tgccctgcaa tgatggaaga cgcagttcaa aggttcaacg
      361 ttcaagagtt caaggtggtc cggaagaaaa ctttgaacct ggaaccttga actgcagcta
      421 tcttatttta aacccaactt atcgaacgtc ccatccttct ccatctcgtc gagaaaacgg
      481 cggtcgatca actgctcggc ttttacttgc tttgcccgcg ggtcggtgcg ggcgatttcg
      541 tctaggtttg cctggatggc gccggccttg atgtccaacc gcggctccag gactttcaac
      601 tcggccgtgt atgccgcgtc gaagactttg cggtcggtca agccgatttt cctgctgatc
      661 actttaaaag ccaactcgcg gtcgctgatc atgatcttca gcgattcggc cataacgtgc
      721 atgaactgac tgatagcttt tggccgttta gcgatcaccg aacgcaaagt aacgaagccc
      781 gtcgccacat atgcgggctt tatctcccgg ccgtcgatga catagttgaa tcctagaaac
      841 gccgcgcggg tgtcctgagg cgtcgtcagt gacgcggcgt caaccgcgcc ggtgctcaga
      901 gcgagaaatg tttcgggagc gccgccggtt tggatcaagt tggcatcctt gagcggatcg
      961 agacccctct ggcgcatgcc atacatcgtg aagtaatgcg cattgccgcc aatgcggccc
     1021 acgccgatac gtttgccttt tagatcttcc ggccgcttga gatccgcctt gcccatcaga
     1081 ctatgggtca acacattttt caccgagccg ataaagacga aatcggtatt gccgctgata
     1141 taggcgcgga tcggcccctc gccgccgacg atggcgacgt tgccgctgcc gccgctcatc
     1201 gccgccgtga cgatacccga cgacgcgatg tagaccagct gaaagtcgag atcgtgtttt
     1261 gcgtaaagcc gcgcttcctc ggcgaaccaa ggcagggcca tcgccacgct cggcgccgac
     1321 tgaaggccga caattcgttc ggcggcgaac gccgggcaac taatccaaaa ataagcgatc
     1381 actagcgaac cgcatcgacg aacgacttgg gtaatcatat cgcgacctcc gtaatcgagt
     1441 acgcgtttta tttttctctt ctttattgtt gcttggcgct taccgctttg acagctgttt
     1501 gatgaaaccc gatgcttcca tttcctggac gaagctagca tcgacaaaac ttttcgggtc
     1561 ggcggccgct gccttgggat tcttcgccgc catgtcgtca agcaacgtct tgatgccggc
     1621 gggatcggca taaggcacgt cgagcaacac ggaagtgtaa ttcttatagg tgcgttccaa
     1681 cccttccgga tcggtgagcc gggtatactt gccgataatc gccttggtgc cttccttgtc
     1741 ggctttgacc aggtgcaacg cttcggtgat tgcgcgagcg aagcggctga ccatcggccg
     1801 ccgagccttg atggtcgttt cgcggaccac cacgccgttg ccatgcaggg gaatcttcaa
     1861 ggttgccata ttcaccaggt cgcggaagcc caaacgctcg gcctctttga taaacggctc
     1921 gttcatgatc gtaaaatgcg tcaagcctcg tgaaatcgat gccagccgtt cgggattgcc
     1981 gccggcgggg acgatcttta cgtccttgtc cggatcgatg cccaagcgtt tgagcgatag
     2041 tttcattccc atgtcggata tgctgccggc gcgattgacc gaaccggttt taccgcgcaa
     2101 ctgctcgaca gtggttatgt tgccggcgct gatgatgtga tcgatgaacg tcgggataat
     2161 cgacaggatc atgacgaaat cggcgccgcc taggcgtgga tttacgacat tgggcgcgga
     2221 agtgatgacg atgtcgagat caccagccac cagcgcctgc atagccggcg gcccggcgcc
     2281 gaagaatatc ggctccgaca ctaagccctg cttggcaaac aagccgcgct cctgcgccac
     2341 ccagagcggt gtatgcgatg ccgtcatcgc tgcccagccg acgcggactt tttcctgcgc
     2401 cgcgctgagc tgcacattta gaatcaagat gccaagcgca agtagcgttc taaccattat
     2461 cgacatgaca cacctcggag aattcaaaga ttacagcgct caggcagacg ctgggaacgc
     2521 cttgacgcgt gagttaatga ttctggtttt ttcggttgca ttgcctaaag cttcgtctta
     2581 gctaatcacc gtttcgacag ctgcttgatg aaccccgacg attccatctc atggacaaaa
     2641 cttgcatcga caaaactctt cggatcagcg cccgcggcct tgggattttt cggcgccatg
     2701 tcgtcgagca acgttttgat gccgacggga tcggcgtagg gtacatcgag aagcaccgcg
     2761 ctatagttcc tgtaagttcg ctctaaaccg tccgggtcgc tgagccgggt atacttggcg
     2821 atagccgcct tggccgcttc tttgtcggct ttcaccagat gaatcgcttc ggtcatcgcc
     2881 cgcgcgaacc ggcttaccat cggccgcctg ctcttgatgg tcgcttcgcg agtaacgacg
     2941 ccgttgatat ggagagagat tttcaacgtc gccaggttga ccaaatcgcg gaagccaagc
     3001 cgctcggcct ctttgataaa cggctcatcc aagatcgtga actgagtgat gcctcttgcg
     3061 atcgacgcca agcgctcagg attgccgccg acggagacga tcttcgcgtc tttctccggg
     3121 tcgatgccaa aacgtttgag cgccagtcgc aggcccatat ccgtaatgct gccggcgcga
     3181 ttgaccgaac ctgacttgcc tttcagttgc tcgaccgacg tgatcgtcgc cgcgctaata
     3241 atgtgatcgg tgaaggtcgg aacgatcgat aggagcatca cgacgtcggc gccaccgagg
     3301 cgtgggttga cgacgttggg ggcggaagtc acgaccatgt ccaaatcacc tgccaccaat
     3361 gcctgcatgg cgggcgggcc cgcgccgaaa aaaatcgcct cgggactcaa tccctgcttg
     3421 gcgaagagac ctttctcgag cgcaacccac agcggcgtgt gtgccgccga taccgccgcc
     3481 caaccaacgc gcaccttctc ctgggcgacg ctaatctgaa catttagaaa caaaattgaa
     3541 agtcctagca tagctttaat aatcgccgcc ataaaacccc tcatagatgg caaattactc
     3601 cgctaaggcg agtgaaccgg aagggcaacc ctggacggct gcgatcagaa ttacacagcc
     3661 gctttacgcc ctttgtaatt tgcgcggcgg ttattcgaag ccgcagatgc tcacggcata
     3721 aagcggccgt cattacttgc cggcacccag cttctgaaag aagcccgtct tctccaaagc
     3781 ctcgacgaat gagttgtcgt aaaagtcgcg cggattggcc ttggccgcag ccggatattc
     3841 tgtctcgtcg agataactga accaggttgt aagctgctcc ggacgcagcg ccagatgggt
     3901 cacgaagtat ggcgcgtaaa aatcataggt gtcatcgatc gccttgacgt cttcagtctg
     3961 ggtgaacttg gcgatggccc gcttggccgc tgccgggtcg tttttggtcc gcgccacgcc
     4021 ttcggcgatt cctttcagga acgccgagat ggtgttgggg ttttgtttga tgaattgttc
     4081 ggtgaagccg tacgccaagg gcaaaccggg aatgttcgcc ttggcgatgt cgactagcat
     4141 gttcaagccg gcgtctttgg ccttcaatgt cgtcggcgcc gatagcgcgc cgcctgccgc
     4201 cttgccgctg acgatcgccg tcaacaccgc cggcacgctc tgcatgtaaa tcaacttgta
     4261 gtcgctgttg aaactcaacc cctggcgctt gagcgcttcg cgcgtagcga tctccagcac
     4321 tgaacgaggc gtcgtgccgg ccagggtctt ccctttcaat tgctggacgc tggcgatttc
     4381 cttggcagtc cagagctgaa agaccagttg cgacatggaa ctgccaacca gtttgatctt
     4441 cgctccctgc aaggaagcgt tgacgagatc gccggccgcc accgacatat cggcggagcc
     4501 tgaaacgacg gattgtaccg agagctgcgg gttgagctgc tgcagtttga cgttgagacc
     4561 gtatttcttg aacagtccgc cgtcgctggc gacgaacagc ggcgtaaaga caccgctcgg
     4621 ctgcgggtag gcgacgacga tttctgattt ttccactgca ccggctgcat caccggcggg
     4681 atcagcgggg
//
LOCUS       DASQKS010000487         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4869516, whole genome shotgun sequence.
ACCESSION   DASQKS010000487 DASQKS010000000
VERSION     DASQKS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4869516"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VE170_12550"
     CDS             <1..842
                     /locus_tag="VE170_12550"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYJ16316.1"
                     /translation="QGFMVLEMLNIAEAWPFHDGSMSQAEMLHFQIAAKKLAFEDRIQ
                     YLEDPAFGDAKIISRLISKEHAAKRRLLVDEILRRPAASAGHQSSDTTYLCAADRDGN
                     AISFIQSVFAPFGSRVIAGDTGVIMNNRLCSFGLDPSKANALKPGKRPAHTLNTYMVF
                     RDGEVFALGGSPGADEQPQTNFQIIHDLVDLDLDPQSAVEAPRWSHMPGTPPRDQLPE
                     ALRMEDGYPNATLDALRAKGHKVAVVDRWSFGSAKVILRDRENGSWLAGADPRRVAYA
                     LGW"
     gene            1164..1655
                     /locus_tag="VE170_12555"
     CDS             1164..1655
                     /locus_tag="VE170_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16317.1"
                     /translation="MFEGWSYSQVLRVIGQSLEERGVEDFSLLWQEGEFLLRCKRVAR
                     LEQHWLDKFLGQKVARSEHNVEIHYSLKSIMWLQIRGEAMRKDANLVPDYFRLSQTLR
                     TVGNYAENRTMPLVSLRRSGGILYLEFEDRSGKKRLEEHGIGSFENYFLRTYLRHRKA
                     RQA"
     gene            1680..>2435
                     /locus_tag="VE170_12560"
     CDS             1680..>2435
                     /locus_tag="VE170_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYJ16318.1"
                     /translation="MERVGMIGTGAMGLALLERLKLAGVGDVTCYDVHAPALEDARRL
                     GFATVASVAALARRATLIDVVVRTDQDMLDCMLGADGVIEAAAPGTLLLLHSSILPHT
                     VKTVEAAARVKAIHVIDACMTGVPDTVRAGKLSFVVGGADELVERATPHLLKMGQEVF
                     HMGPAGTGAVGKLIKNMLGGAESLIVHEAIQIGIAGGIPYPKALEMMRRIGHDSVLNR
                     WQRTFDPSGSDPLPKAGRNVLNKDIPLVAELARM"
BASE COUNT          482 a          684 c          761 g          508 t
ORIGIN      
        1 cccagggctt catggttttg gaaatgctca acatcgccga ggcctggccg tttcacgatg
       61 gcagcatgag ccaggcagag atgctgcatt ttcaaatcgc ggcgaaaaag ctggcctttg
      121 aagaccgcat ccaatatctc gaagatccgg cgtttggcga tgctaagatt atttctcgat
      181 tgatctccaa agagcacgcg gcgaagcggc ggctgctcgt cgacgaaatc ctgcgccggc
      241 cggcggcgtc ggcgggtcat caaagcagcg acacgactta tctttgcgca gccgatcgcg
      301 acggcaacgc gatctcgttc attcagagcg ttttcgcacc ctttggctcg cgggtgatcg
      361 ccggtgacac gggcgtgatc atgaacaacc ggttatgcag ttttggtctc gatccgagta
      421 aggccaatgc gctcaagccc ggcaaacggc cggcgcacac gctcaacacg tacatggtgt
      481 tccgcgacgg cgaagtcttt gccctgggcg gtagccccgg cgccgacgaa cagccgcaaa
      541 ccaactttca gatcattcac gacctggtcg atctcgacct tgacccgcag tccgctgtcg
      601 aagcgccgcg ctggagccac atgcccggta cgccgccgcg ggatcagctg cccgaagcct
      661 tgcgcatgga agacggctat ccgaacgcga cgctcgatgc cttacgtgcc aagggccaca
      721 aagtcgccgt cgtcgaccgt tggtccttcg gtagcgccaa ggtcatcctg cgcgaccgag
      781 agaatggttc gtggctcgcc ggcgccgatc cgcgccgggt agcctacgct ctgggttggt
      841 gagcttgctt ggcaaaacag tttgcccaac gtcaatgccg ctggtaaggc ctcgctcaac
      901 cgcaccaatc atgcagcggg gaagaatgcc aagttcgacc ccgttcttcg acaattccgt
      961 gatcgccgca gtggaaaagc ccgcgagcat tgatcgacac tacaaacaat agctgctatc
     1021 tggatggtta attgtcgggg tgccgatcgc tgccctggtg ccgcgtgcta ctggcatgat
     1081 ggccgggcta cggccgttgg cgctctgagg atttgctctg tgaattttgc cttggcttcg
     1141 tagctgcgga gttttacttg tttatgtttg agggctggtc ttattcccag gtcttgcgcg
     1201 tcatcggaca atcgctggaa gagcgcggcg tggaagattt ctcgctgcta tggcaggagg
     1261 gcgagtttct cttgcgctgc aaacgagttg cgcggttgga gcaacattgg ctcgacaaat
     1321 tcctcggtca aaaggttgcc cgcagcgagc ataacgtgga aattcattac tcgttaaaga
     1381 gtatcatgtg gctgcagatc cgcggcgagg cgatgcgcaa agacgccaat ctggttcccg
     1441 attattttcg cctgtcgcag actctgcgca ccgtcggtaa ttacgcggaa aatcggacaa
     1501 tgccgctagt gtccttgcgg cgcagcggtg ggattttgta cctcgagttt gaagaccgta
     1561 gcggcaaaaa acgcctcgag gagcatggca ttgggtcgtt tgagaattat tttctgcgca
     1621 cctacctgcg ccatcgcaag gctcgccagg cttgaagcgc cacgggcgaa ggattggcaa
     1681 tggaacgagt tgggatgatc ggtacgggcg ccatgggttt ggcgctcttg gagcggctca
     1741 agcttgccgg cgtgggcgat gtgacctgtt acgacgttca tgcaccggcg ctggaggatg
     1801 cgcgcagact gggttttgcc acggtcgcca gcgtggcggc gctggccagg cgtgcgacgc
     1861 tgatcgacgt ggttgtgcgt accgaccaag atatgctgga ttgcatgctt ggcgcggacg
     1921 gggtgattga ggcggccgca ccggggacgc tcttgctgct ccacagttcg attctgccgc
     1981 acacagtcaa gacggtggaa gccgcggcgc gtgtcaaagc cattcacgtc atcgacgctt
     2041 gcatgaccgg cgtgccggac accgtgcgcg ccggcaagct aagttttgtg gtcggcggcg
     2101 ccgatgagtt ggtcgagcgc gccacgccgc atctgctcaa gatgggccag gaagtgtttc
     2161 acatgggacc ggcgggcacg ggagccgtgg gtaaattgat caagaacatg ctcggcggcg
     2221 ccgaatctct gatcgtccat gaagcaattc agatcggtat cgccgggggc attccgtatc
     2281 ccaaagcgct ggaaatgatg cggcgcatcg gccatgacag tgtcctgaat cgctggcaac
     2341 gcaccttcga tccaagcggc agcgatccgc tgccgaaagc aggacgcaat gtattgaaca
     2401 aagacattcc actagtggcc gagctggcgc gcatg
//
LOCUS       DASQKS010000488         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4870297, whole genome shotgun sequence.
ACCESSION   DASQKS010000488 DASQKS010000000
VERSION     DASQKS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4870297"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VE170_12565"
     CDS             complement(<1..1164)
                     /locus_tag="VE170_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ16319.1"
                     /translation="MTLTGCKSANKSTSDSHFRANLGTEPPSLDWSIATDHVSFNVIA
                     NLMVGLTEFDKELKPAPVIAKSWELFDGGKKIVFKLRDDVQWSDGKKVRAQDFEYSWK
                     RLLDPKTASQYAYILFDIANAAEYAQGKLKDPAQVGVTAADDQTLVVTLRHPAAYFLA
                     ITTFEVTYPQRQDIIETHDNRWTQPGKIVTNGPFLLASWKQQNEIELRANDKYFRGKP
                     ALDKVTLYMVNEKTTALTMYEQGNLDFMDESHSIPTLDKPRLAKVPEYKLVPQLRGEY
                     YGFAVDRKPFDNPKLRRAFARAIDREVFPKILQGGKTPATSWIPPGMLAHNPKIGLAY
                     DPNEARRLLADAGFPDGKGLPPILLGYNTQEDHKLVAEAIQAMWQKNLNVVVKI"
     gene            1291..2322
                     /locus_tag="VE170_12570"
     CDS             1291..2322
                     /locus_tag="VE170_12570"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HYJ16320.1"
                     /translation="MDAKELLMSSLVIEGHRDVYEQIYRKSVGEEAPIRDAVAPRLIR
                     DGINMTVYAICGDSYSHSQNTGRYLETALEQIDHFLEEAPRSEGMIQMVKSRGDLPER
                     PQPGSVKFLLHFEGCMPLRGSLENLRNFYRLGLRSIQPVWNFRNELGDGVWENRTGGG
                     LTNFGVQVIKEANRLGMVVDLSHMNREGFFQTLDAATAPLLVSHANACGMLKNPRNLD
                     DEQIKAIARQGGLVGILALPERVGEGEVGIEDMLKHMDYMIKLVGIEHLALGMDFVKY
                     DGPRTLKDRHHPLHKDPLIKGFEEIEDLPNLIDGLQRHGYKDEEIRMILGGNYLRVLK
                     TILPEQSVI"
     gene            2557..>4729
                     /locus_tag="VE170_12575"
     CDS             2557..>4729
                     /locus_tag="VE170_12575"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ16321.1"
                     /translation="MTIIGDKVPRVDGVDKVTGAAKFTGDLPFPDLLEAKVLRSPLAH
                     AKIISIDPSEAAMLPGVAAILTREDLKDIDPYYGNCLRDRAVVATDKVRFVGEPVVVV
                     AAESGLIAEQALALIDVQYQELPCVGDIDRARAEGAPLLHETQSGAGEFHDVAGVGEI
                     FGGNICHRERFVKGDPDQAFVGADAIVDETFTFPMIYQYAMEPHSAVARVNAAGITLW
                     SSSAHPFLVRSELAHMFGYPHAKVEVIVPYVGGAYGSKSYFKIEPLAVAIARKTGGRP
                     VRVVQSVTESMLTTRRHSARLRIKTGVKRDGTLIARDAEVLMDTGAYADNGPRVAKRV
                     ISRMLGPYKLDHCKIDVVAFYTNTVPAGSMRSIGGPQSIWALESHMDTIASRLGIEPL
                     EYRMRLLLKRGAVLKPGATAIDADLRQGMQAAVAPLDWQHRSRSRSQGVGVAVGVSDS
                     EAMPVSVALVRLLADGSVVLLAGSTEVGQGARTVLCQIVAEELSVSVDRVSMRGTETL
                     TTPFDRSTGASRSTTVMGSAVKAAAADLRSQLIEAAAEAFDTSPKSVVLRNGEACCDD
                     KSLSYAQVVSKFFGMPGGELIGRGYMRPDGALGKTFPLFWETGMGGAAISLDRETGVI
                     KLEKYVTVADVGRAINPVQAEGQDEGATIQGLGNTLCESLEYDNGQPLNANLVDYRVP
                     RFTDLPETFESGLIENHDGPGPYGAKGMGESGIVCVAPAVGN"
BASE COUNT         1014 a         1324 c         1406 g          985 t
ORIGIN      
        1 gatcttcacc accacgttca aattcttttg ccacatcgct tgaatcgctt cagcgaccaa
       61 cttgtgatcc tcttgggtat tgtacccgag cagaatgggc ggcaaaccct tgccatccgg
      121 gaatcccgcg tccgccaaca gccggcgggc ttcgttgggg tcgtaggcca aaccgatctt
      181 tgggttatgc gccagcatgc ccggcggtat ccatgaagtt gccggagttt tgccgccctg
      241 gagaatcttc ggaaaaactt cgcgatcgat ggcgcgagca aaggcccggc gcaatttggg
      301 attatcgaaa ggcttgcggt caacggcgaa accgtaatat tcaccgcgca actgcggcac
      361 cagtttgtat tcaggcactt tggccaagcg cggcttgtcg agagtcggga tgctatggct
      421 ctcatccatg aaatccaaat tgccctgctc gtacatggtc agcgcggtgg tcttctcgtt
      481 gaccatatac agggttactt tatccaacgc cggtttgccg cggaaatact tgtcgttggc
      541 gcgcagttcg atttcattct gctgcttcca tgacgccagg agaaaaggcc cgttggtgac
      601 gattttgccg ggctgggtcc agcggttgtc atgggtttcg atgatatcct ggcgctgcgg
      661 ataggtgact tcgaaggtgg tgatcgcgag aaagtaggca gccgggtgcc tcaaggtaac
      721 gaccaaggtt tgatcgtccg ccgccgtgac gccgacttgc gccgggtcct tgagttttcc
      781 ttgggcgtat tcggcggcgt tcgcgatgtc aaacaagata taggcgtatt gcgaagcggt
      841 cttgggatcg agcaagcgtt tccatgaata ctcaaagtcc tgggcgcgga cctttttgcc
      901 gtcgctccat tgaacgtcat cgcgcagctt gaaaacgatc tttttacccc cgtcaaaaag
      961 ttcccaagac ttggcgatca ccggcgccgg cttcaactct ttatcgaact cggtgaggcc
     1021 gaccataaga ttggcaataa cgttgaatga aacatggtcg gtggcgatgg accaatcgag
     1081 gctcggtggt tcggtgccga gattggcgcg aaaatggctg tcggaagtgc tcttgttggc
     1141 ggacttacag ccggttagcg tcatcaagac gatgacacat ataaccaaaa atggctgggg
     1201 gtggcggggc gttatcattc ttgcgtgtcg gtggtttagc cgatattact agcgcagtac
     1261 gctgattcct tcaagaaagc gcaggcatct atggacgcaa aagaactttt aatgagcagt
     1321 ctggtgatcg aaggacaccg cgatgtctac gagcaaatct accgtaagtc ggtcggcgaa
     1381 gaagcgccga tccgcgacgc ggtggcgccg cgcttgatcc gcgatggcat taatatgacc
     1441 gtctacgcga tttgcggcga ctcctattcg cactcgcaga acaccggccg ctatttggaa
     1501 accgccctcg aacagattga ccactttctt gaagaggcgc cacgctccga aggcatgatc
     1561 cagatggtta aaagccgcgg cgacctaccg gaacggccac aacctggcag tgttaaattt
     1621 ttactccatt tcgaaggctg tatgccgttg cgcggcagcc ttgagaacct gcgcaatttt
     1681 tatcgcttgg gtctgcgctc gatccagccg gtctggaatt ttcgcaacga gttgggcgat
     1741 ggtgtgtggg aaaaccgcac gggcggcggc ctgaccaact ttggcgtcca ggtcatcaag
     1801 gaagccaacc gcttgggcat ggtggtcgat ctgtcgcata tgaatcgcga aggtttcttt
     1861 caaaccctgg acgccgccac cgcgcccttg ctggtgagtc acgccaacgc ctgcggcatg
     1921 ctcaagaacc cgcgcaattt agacgacgaa cagattaagg ccatcgccag gcagggtgga
     1981 ctggtcggca ttttagctct gccggaacga gtcggcgaag gcgaagtcgg catcgaagac
     2041 atgctcaaac acatggatta tatgatcaag ctggtcggca tcgagcatct cgcgctgggc
     2101 atggacttcg tcaagtacga cggcccgcgc acactcaagg accgccacca tccgctgcac
     2161 aaagatccgc tgattaaagg cttcgaagag atcgaagatc tgccgaacct aatcgacggc
     2221 ctgcaacgcc acggctacaa agatgaagag atcagaatga tcctgggcgg caattatctg
     2281 cgggtattga agacgatctt gccggagcag tcagtcatct agcacggcgc aaattcaatc
     2341 accacgcgaa gccgcaagcg aaaagaagat ggcgcaaagt acgcaaagac cgcaaaaaat
     2401 gattcgggag ttgtggagtg gatttgaaaa ccgccccttg gatttggtct atcgagaagc
     2461 ttgcgcaacc ccgcaagcga tcacggatag tcatatgatg acggtaaaat ttgtcaggaa
     2521 gtttgccgca cttcgaagcg agctattgac tctaatatga cgataatcgg cgacaaagtt
     2581 ccccgcgtcg acggcgtcga caaagtcacg ggcgccgcga agtttaccgg cgatctaccg
     2641 tttcccgact tgctcgaagc caaggtgctg cgcagcccac tggcccacgc caagatcatc
     2701 tcgatcgatc ccagcgaagc tgcgatgctc cccggcgtcg cggccattct cacgcgcgag
     2761 gatctcaagg acattgatcc ctattatggt aattgcctgc gcgatcgcgc ggtggtcgcc
     2821 accgacaaag tacgcttcgt cggcgaaccg gtggtggtgg tcgccgctga aagcggcctg
     2881 atcgccgagc aagcgctggc actgatcgat gtgcaatatc aagaactgcc ctgtgtcggg
     2941 gatatcgatc gcgcgcgggc cgaaggcgca ccgctgttac acgagactca gtcgggcgcc
     3001 ggcgagtttc atgatgtcgc tggtgtgggt gaaatctttg gcggcaatat ttgccatcgc
     3061 gagcgcttcg tcaagggcga tcccgaccag gccttcgtcg gggcagacgc gatcgtcgac
     3121 gagactttca catttccgat gatctatcag tacgccatgg aaccgcacag tgcggtggcg
     3181 cgggtcaacg cggctggtat tacgttgtgg agttcatcgg cccatccgtt tttggtgcgt
     3241 tcagagttgg cgcatatgtt tggctacccc cacgccaaag tcgaagtgat cgtgccctat
     3301 gtcggcggcg cctatggcag caagtcctat ttcaaaatcg aaccgctcgc cgtcgccatt
     3361 gcgcgtaaga ccggcggccg tccggtgcgg gtggttcaaa gtgtgaccga gtcgatgctg
     3421 acgacccggc gacattcggc gcggctgcgc atcaagaccg gcgtcaaacg cgacggtact
     3481 ctgatcgccc gcgacgccga agtgctcatg gataccggcg cctatgccga caacggaccg
     3541 cgagtggcca aacgagtgat cagccgcatg ctcggaccgt acaaactcga ccattgcaag
     3601 atcgatgtcg tggcgttcta caccaatacc gtgccggcag gttccatgcg ctccatcggt
     3661 ggaccccaat cgatctgggc tttggaatcg cacatggata cgatcgccag tcgccttggc
     3721 attgagccgc tggaatatcg catgcgcttg ttgctcaagc gcggcgcggt gctgaagccg
     3781 ggtgccacag cgatcgatgc cgacctgcgc caaggaatgc aagccgcggt tgctccgctc
     3841 gactggcaac accgcagtcg cagcagatcc cagggcgtcg gtgtggccgt cggcgtatcc
     3901 gactccgaag cgatgccggt ttcggtagcg ctggtgcgtt tgcttgccga cggcagtgtc
     3961 gttctactcg ccggcagcac cgaagtgggg cagggcgcca ggacggttct ttgccaaatt
     4021 gtcgccgagg aattgtccgt ttccgtggac cgcgtcagca tgcgtggtac cgagacactg
     4081 accacgccat tcgaccgctc caccggcgcc agtcgctcga cgaccgtcat gggcagcgcc
     4141 gtcaaagccg ccgccgcgga tttgcgcagc caattgattg aagccgcggc ggaagccttc
     4201 gacacgagtc cgaagtcggt cgtcctgcgc aacggcgagg cgtgttgcga cgataaatca
     4261 ttgagctacg cccaagttgt cagtaaattc ttcggcatgc ccgggggcga actgatcggc
     4321 cgcggctaca tgcgccccga cggcgcgttg ggcaaaacct ttccattgtt ctgggaaacc
     4381 ggcatgggcg gtgcggcaat ttcgctggac cgggaaaccg gcgtaatcaa actggaaaag
     4441 tacgtgaccg tcgcggatgt cggtcgcgcg atcaatccgg tgcaggcgga aggccaagac
     4501 gaaggggcaa cgattcaagg attgggcaat accctctgtg aatctctcga atacgacaat
     4561 ggtcaaccgc tcaacgccaa tctggtcgac tatcgagtgc cgcgcttcac cgacttgccc
     4621 gaaacgttcg aatccggctt gatcgaaaat cacgacggcc ccggcccata cggtgccaag
     4681 ggcatgggcg agtcagggat cgtttgcgtc gcgcccgcgg tgggcaacg
//
LOCUS       DASQKS010000489         6691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5724942, whole genome shotgun sequence.
ACCESSION   DASQKS010000489 DASQKS010000000
VERSION     DASQKS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5724942"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VE170_12580"
     CDS             <1..489
                     /locus_tag="VE170_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ16322.1"
                     /translation="SGKILIAGTDVTELSETELAVWRSRNVGFIFQFYNLIPVLTALE
                     NVELPLILTPLAKKARRDHAEMALAVVGLAERMHHYPRQLSGGQEQRVAIARAIATDP
                     AILVADEPTGDLDKASAEEVLELMDRLNRELNKTILMVTHDPRAAERARAVRQLDKGE
                     LA"
     gene            579..1739
                     /locus_tag="VE170_12585"
     CDS             579..1739
                     /locus_tag="VE170_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYJ16323.1"
                     /translation="MELIRLLFRNVLRHKLRGMLTIVGVGVAVMAFALLQTVVSAWYA
                     GVEASAANRLITRHAVSFVFPLPLAYRDRIAQVPGVNKVTFASWFSGVYIDKNQFFAR
                     LAVDSDTFFDVYSEFLVPPEQFEVFKRERNATIIGSDIAERYNLKLGDIMPLEGDVFP
                     GQWEFVVRGIYQPRDQTTDPTTMMFHYKYLEERVRQEMPQRSGEIGWYIVRIDDPENS
                     AAISQEIDSLFANSRAETKTETERAFQQSFLSAASAIITAMNIMSFVIIGIILLVVGN
                     TMIMSARERTHEFAVLKALGFRGSQLFFLIAGESILLALFGSALGLAVTFPAVAGFET
                     ALPKGWFPVFYIRPETVAIGCAAGLAVGALAALIPLRRVLTTRIVDGLRHVG"
     gene            1717..2916
                     /locus_tag="VE170_12590"
     CDS             1717..2916
                     /locus_tag="VE170_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ16324.1"
                     /translation="MASVTSGERLMQLPLKYIIRSSVARRLTTVITILGVALVVFVFT
                     AVLMMANGVQKTLRSTGSDDNLLVARKAAMSEIMSIMDREAAGIIVNLPQVAQAADGT
                     PMSSKEVVVIINLNKLGGAGISNVTVRGVEPPAFQLRPQVKITEGRMFNWGAREVIAG
                     AGITKRFVGAQIGEKVKFGGDIWTVVGLFDAAGSAFDSELWGDLNQIADAFKRSSFST
                     ITVRLKDPDDLAGVTRAFESDNRLQYFVGKREKKFFEEQSEMMATFIRILGIFITVIF
                     STGSTIGAMITMYGSVANRTTEIGTLRALGFFRRSILVAFLLESLALGFAGGLAGVGF
                     ASVLQFFSISTLNFGSFSELAFSFALSPSIVATSLGFSLLMGLIGGFLPAVRAARLDI
                     IQALRAV"
     gene            complement(3255..4688)
                     /locus_tag="VE170_12595"
     CDS             complement(3255..4688)
                     /locus_tag="VE170_12595"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYJ16325.1"
                     /translation="MALTRREFLLRSFGALGAATLAMERFGMTDVFAQAADYKALVCV
                     FLFGGNDSDNILIPYDNYSQYAVKRNNSANVGIPQASLLQITPSSGLGGAKFGLHPSL
                     DGIKSLWDLGKVAILPNTGPLVEPLTRNEYRNRLKRIPLNLFSHSDQENQWQTAVANG
                     FVSTGWGGRTADAIASLNGSATFPMIATMTGNNIFSTGAFRRPLALSPAPSTLSRVIR
                     LDGFPNPPASLQGIPRYDAMEAIQALDDGFTVVKAANQINASTFSTMEALRSAPDPVV
                     SPFPLSPRTGLGNQLEQVAKVISLHDALGLKRQIFFCSIGGFDTHGDQVNGNDPTTGQ
                     HANLWATIDNAVKAFYDATVGLGVQNNVTLCTMSEFGRTYVPNGGLGTDHAWGNHVFV
                     VGGAVNGGNFYGVPGPNGTVFPTLAAAGPNDTDNGSGARGRWIPTTAIDQVATTLASW
                     MGVANADLAGISPNIANFSTANLGFMS"
     gene            complement(4703..6391)
                     /locus_tag="VE170_12600"
     CDS             complement(4703..6391)
                     /locus_tag="VE170_12600"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HYJ16326.1"
                     /translation="MIGENGLAAKLLFYSLVAIMGVTDAGNSYAAVAPKATEAEAVRF
                     LQQASWGPNKATIQKVRQQGIAAWIDGQLKARPKTYPNLRFYPESQPENCVDACARDN
                     YTYYQLQKHFFTNALTGQDQLRQRAAFALSQIFVTAQGAVPMPAWMRTYQQLLYDNAF
                     GNYRELLFDVALTPAMGRYLDMLNNRCQTPVPANVNICRNGSIAQPNENFAREILQLF
                     SIGTFMMNQDGTYQTDGVGQPIPTYDQAKVEEFARVFTGWVLAPPLRGVGGDVPNYKV
                     PMVVRRNSQNQEMHHDRGAKTLLNGAVIPAGTDAEVELNLAIDNIVSHANVAPFISKQ
                     LIRHLVTANPSPAYVGRIAAIFSANLNSANQLGVVIKAILMDPEARTLPDAAVDPDAG
                     KLNEPVVFMLNFLRAFEAKSDGVLNGGSRGSAAMNQDVFRSPTVFNYYPAEYEVPGEP
                     NLIGPVFGIFSTQTTITRANFINQVLFSGIPVSQPNTPKGTVLTLKIWEDLADTPVEL
                     VDRLSCWMLHCTMSQDMKTIIVNAVNTVPVTNLLLRAQTAIYLVATSNHYQVLR"
BASE COUNT         1357 a         1794 c         2000 g         1540 t
ORIGIN      
        1 tcggggaaaa ttctcatcgc cggcaccgat gtgacggaac taagtgagac cgaactggcc
       61 gtctggcgtt cgcgcaacgt tggcttcatt tttcaatttt acaatttgat ccccgtgctc
      121 accgcgttgg aaaacgtcga gctgccgctg atcctgacgc cgctggcgaa aaaagcgcga
      181 cgcgaccatg ccgagatggc actcgccgtc gtcggcctgg ccgagcgcat gcatcactat
      241 ccgcgccaac tttccggcgg acaggaacaa cgggtagcga tcgcccgcgc catcgccacc
      301 gatccggcga tcctggtggc cgacgaaccc accggcgatc tcgacaaagc gtccgccgag
      361 gaagtgctcg aactcatgga tcggctcaac cgcgagctca acaaaaccat tctcatggtc
      421 actcacgatc cccgcgccgc cgagcgggcg cgcgcggtgc gtcagttgga taagggcgag
      481 ctggcataac gaaatcgttc aaaagttcaa ggttcggttc aacgtcagat tccgagccga
      541 ggcagtgctt cctgagtcaa acaacgtaaa gcaacgccat ggaattgatc aggttgttat
      601 ttcgcaatgt cttgcgccat aagctgcgcg gcatgctcac tatcgtcggc gtcggtgtgg
      661 cggtaatggc gtttgctttg ctacagaccg tggtgagcgc ctggtatgcc ggcgtggaag
      721 cttcggcagc gaaccggtta atcacgcggc acgcggtctc gttcgttttt ccgttgccgc
      781 tcgcctatcg cgaccgcatc gcgcaggtac ccggggtcaa caaagtgacc ttcgccagtt
      841 ggttctccgg ggtttatatc gacaagaatc aattcttcgc ccgtctggca gtcgattccg
      901 ataccttctt cgacgtctac tcggagttcc tggttccgcc ggaacagttc gaagtcttca
      961 agcgcgaacg taacgcaacg atcatcggca gcgacatcgc cgagcgttac aacttgaagc
     1021 tcggcgacat catgcccctc gagggcgatg tctttccggg ccagtgggag ttcgtcgtgc
     1081 gcggcattta ccaaccgcgc gatcaaacca ccgacccgac gaccatgatg tttcactaca
     1141 agtatttgga agagcgggtg cgccaggaaa tgccgcagcg ctcgggagaa atcggctggt
     1201 acatcgtccg tatcgacgat cccgaaaatt cggcggcaat ttctcaggaa atcgacagtc
     1261 tgttcgccaa ctcccgcgcc gaaaccaaga ccgaaaccga aagagctttt cagcagagtt
     1321 ttctttccgc ggcgagcgcg atcatcaccg cgatgaacat catgtcgttt gtcatcatcg
     1381 gcatcattct attggtggtc ggcaatacca tgatcatgtc agcgcgggag cgaacccatg
     1441 agttcgcggt gttgaaggcg ctgggatttc gcggcagcca gttatttttc cttatcgccg
     1501 gcgaatcgat ccttctcgcg ctgttcggca gcgctttggg actggccgtg actttcccgg
     1561 cagtggcggg ttttgagacc gcactcccca aaggttggtt tccggtgttc tatataaggc
     1621 cggaaaccgt ggcgatcggc tgcgctgccg ggctggcggt cggcgcactg gcagcgctta
     1681 ttccattgcg ccgggtgctc actacgcgca tcgtcgatgg cctccgtcac gtcgggtgaa
     1741 cgcctcatgc agttgccgtt gaaatatatc atccgcagtt cggtggcgcg ccgcttgacc
     1801 accgtgatca ctatcctcgg tgtcgccctg gtggtgttcg tctttaccgc tgtgttgatg
     1861 atggccaacg gcgtgcaaaa gactttgcgc tccaccggtt cggatgacaa tttgctggtg
     1921 gcacgcaagg ccgccatgag cgagatcatg agcatcatgg accgcgaagc ggcgggcatc
     1981 atcgtcaatc tgccgcaagt ggcgcaagcc gccgacggca cgccgatgag ctcgaaagag
     2041 gtcgtggtca ttatcaattt gaacaagctc ggcggcgccg gtatcagcaa tgttaccgtg
     2101 cgcggcgtcg aaccaccggc gttccagttg cgcccgcagg tgaaaatcac cgagggcaga
     2161 atgttcaact ggggcgctcg tgaagtgatc gccggcgccg gcatcaccaa gcgctttgtc
     2221 ggcgcgcaga ttggcgagaa ggtcaaattt ggcggcgata tctggaccgt ggtcggactg
     2281 ttcgatgccg ccggcagcgc cttcgattcc gagctgtggg gtgatttgaa tcagatcgcc
     2341 gatgccttta agcgctcatc cttttcgacc atcacagtac ggctcaaaga tcccgacgat
     2401 ctcgccggtg tgacccgggc ttttgaaagc gacaatcggc tgcaatattt cgtcggcaag
     2461 cgtgaaaaga agtttttcga agagcagtcg gagatgatgg cgacctttat acgcattctg
     2521 gggattttta tcacggtgat cttcagcacc ggctcaacca tcggcgcgat gattacgatg
     2581 tacggttccg tggcgaatcg aacgaccgaa attggcacct tgagggcgct cggttttttc
     2641 cgacgcagca ttcttgtcgc gttcttattg gagtccttgg cgctggggtt tgccggcgga
     2701 ttggcgggag tcgggtttgc ctctgtgctc cagttttttt cgatatcgac gttgaacttt
     2761 ggctcattct ccgagctggc tttctccttc gctttgtcgc cgtcgatcgt cgcgacgtcg
     2821 ctgggatttt cactgctgat gggactgatt ggcggctttc ttcccgcggt gcgggccgcg
     2881 cggctagata ttatccaggc gctgcgcgcg gtgtgaatcg aattcgcagg atttagccgc
     2941 aatgagaatt caccacgaac cgcgagctcg gcgaagccgc gattgtaaag aaaaatggcc
     3001 gcaaacaacg caaaaataga atcgggaatt gcgggccagg tttgaacccg gccgttcagc
     3061 tatcgtggtt agtagtctta aattcgagtt ctaattcatt cgaatccttc gactccatac
     3121 atagtgcgat gaatgtcgtt cgttgccgaa caccagcagg ttatttgagg ttattgtggg
     3181 agacaatgcg cctcggtagc gcgaaatcat aactgggttt cgcgttaccg aggcttcgat
     3241 tcgtattaag tgtgctagct catgaatcct aggttcgcgg tgctaaagtt ggcaatgttg
     3301 gggctaatgc cggctagatc cgcgttggcg acccccatcc aactcgccaa ggtcgtcgca
     3361 acttgatcga tggctgtcgt cgggatccat cggccgcgcg cgccgctgcc gttgtcggtg
     3421 tcattcggtc cggcggcagc cagggtcgga aagacggtgc catttggacc ggggacgccg
     3481 taaaagttac cgccgttgac cgcgccaccg acgacgaaaa catggttgcc ccaagcgtga
     3541 tcggtcccta gtcccccgtt gggcacatag gtgcggccga attcgctcat tgtgcatagt
     3601 gtgacgttat tctgcacccc caatcccact gtggcgtcgt aaaatgcttt gacagcgttg
     3661 tcgatggtcg cccacaggtt ggcatgctgg ccggtagtcg gatcgttgcc gttgacttgg
     3721 tcgccatggg tatcgaagcc gccaatggaa cagaaaaaga tctggcgttt gagaccaagc
     3781 gcgtcgtgca ggctgatcac tttggccacc tgttctaact gattgccgag tccggttcgc
     3841 ggactgagag gaaagggtga caccaccgga tcgggtgccg agcgcaaagc ctccatcgtc
     3901 gaaaaagtgc ttgcattgat ttgattggcg gccttgacca ccgtaaagcc gtcgtcaagg
     3961 gcctgaatcg cttccatggc atcgtaacgt ggaatacctt gcagcgaagc tggtggattg
     4021 ggaaaaccgt ccagtctgat gacccggctc agtgtagatg gggcagggga gagtgcgagg
     4081 ggccggcgga aggcgccggt gctgaaaata ttattaccgg tcatcgtcgc gatcatcgga
     4141 aacgtggcgc tgccatttaa actggcgatg gcatccgcgg ttcggccgcc ccatccggtc
     4201 gacacgaagc cgttggccac cgcggtctgc cattggtttt cctggtcgga atgggaaaac
     4261 agattcagtg gaattcgttt gaggcgatta cggtattcgt ttcgcgtcaa tggttccacc
     4321 aacggcccag tgttggggag gatcgcgacc ttgccgaggt cccagaggga cttgatgccg
     4381 tcgagcgacg ggtgcaggcc gaacttcgct cctcctaatc cactgcttgg agtgatttgc
     4441 agtaagctgg cctgcgggat gccgacattc gccgagttgt ttcgtttaac cgcgtactgg
     4501 gaataattgt catagggaat aagtatatta tcgctgtcat tgccgccaaa gagaaatacg
     4561 cataccaacg ctttataatc cgcagcctga gcaaagacat cggtcatgcc gaagcgttcc
     4621 atcgccaggg tcgccgcgcc cagggcgccg aagctgcgca atagaaattc tctgcgtgta
     4681 agtgccatat aaatctcctt cgttagcgta gaacttggta gtggttcgat gtcgcgacga
     4741 gatagatcgc tgtctgcgcg cgtaagagca gattcgttac cggcaccgtg ttcacagcgt
     4801 taacgataat ggtcttcata tcctgtgaca tggtgcagtg aagcatccag cagctcaatc
     4861 ggtcgaccaa ctccaccggc gtgtcagcaa ggtcttccca gattttaaga gtcagaacgg
     4921 tgcccttcgg cgtgttcggc tggctcaccg ggattcccga gaaaagcact tgattgatga
     4981 agttagcccg cgtaatcgtc gtttgcgtgg aaaaaatgcc aaataccggg ccgattagat
     5041 taggttctcc gggaacttcg tattccgccg gataatagtt aaagaccgtc ggcgagcgaa
     5101 acacatcctg gttcatcgcc gccgaaccgc gcgagccgcc gttgaggacg ccatcgcttt
     5161 tggcctcgaa ggcgcgcagg aagttgagca taaacactac cggttcgtta agtttaccgg
     5221 catcgggatc cactgccgca tcgggtagag tgcgcgcttc ggggtccatg agaatcgctt
     5281 taatcactac ccccagttgg ttggcgctgt tgaggttcgc ggaaaatatc gccgcgatcc
     5341 ggccgacgta ggcgggggac gggttggccg tgaccaagtg gcgaatcaac tgtttggaga
     5401 tgaacggcgc gacgttggcg tggctgacaa tgttgtcgat ggccaaattc aattccacct
     5461 cggcatcggt gccggccggt atgaccgcgc cgttaaggag tgtcttggca ccgcggtcgt
     5521 gatgcatttc ctggttctgg ctgttgcgcc gcaccaccat agggactttg tagttgggaa
     5581 cgtcgccgcc gacaccgcgt agcggcggag cgagtaccca gccggtaaat acgcgggcaa
     5641 attcctcgac cttggcttga tcgtaggttg gaatcggctg gccaacgccg tcggtttggt
     5701 aagtgccgtc ctgattcatc atgaaggtgc cgatggagaa tagttggagg atttcccggg
     5761 caaagttttc gttgggctga gcgattgaac cgttgcggca aatattaacg ttggcgggaa
     5821 ccggtgtttg gcagcggttg ttgagcatgt cgaggtagcg gcccatagcc ggagttagcg
     5881 cgacatcgaa gagcaactcc cggtagttgc cgaaggcgtt gtcgtacagc aattgctggt
     5941 aggtgcgcat ccatgccggc atgggaacgg ctccctgcgc ggtgacgaat atctggctca
     6001 acgcgaaggc tgcgcgttgg cgcaattgat cctgccctgt gagagcgttg gtgaaaaagt
     6061 gcttctgcag ttgataatag gtgtagttgt cgcgcgcgca ggcgtcgacg caattctccg
     6121 gctgggattc agggtagaat ctgagattag ggtaagtctt gggtcgggct ttgagttgcc
     6181 catcgatcca tgcggcgatg ccttgctgcc ggactttttg tatggttgcc ttattgggcc
     6241 cccaactcgc ttgctggaga aaccgaactg cttcagcttc tgtagccttt ggcgccacgg
     6301 ctgcgtaaga attgccggcg tccgtcaccc ccataatcgc cactaacgag tagaacaaga
     6361 gctttgcggc aagaccgttc tctccgatca tgtttcctcc tctttgtgcg cctgtggtcg
     6421 ccttcttaac actgcgaccc gaagctggcg ttgttgatca caaacgcgct ggtctaatcg
     6481 atctcgctag aggctgagca aaatcaggac ccatgagaga gcggtggcag ttgggcgggc
     6541 gtgacttgga atgacaaggg acatcgacta ttcgcgcagc gggaaacgtt cgttggcggc
     6601 catttgtgca ccactgccga tatttctgtt gataccggtg gggtaattcg gtgcgccgaa
     6661 atgaaaggtg gaatgctcga agcgttcgat g
//
LOCUS       DASQKS010000490        18638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1944767, whole genome shotgun sequence.
ACCESSION   DASQKS010000490 DASQKS010000000
VERSION     DASQKS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 18638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18638
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944767"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /gene="dapB"
                     /locus_tag="VE170_12605"
     CDS             <1..370
                     /gene="dapB"
                     /locus_tag="VE170_12605"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HYJ16327.1"
                     /translation="DDYDVEIVEAHHRFKKDAPSGTALALGRTVAKALNRDLDKVGIN
                     GRKGIVGARGKKEIALLSVRAGDIIGEHTVIFGGIGERLEFIHRAHSRDTFAHGAIRA
                     AQWLARRKPGLYGMQDMLGW"
     gene            498..1874
                     /locus_tag="VE170_12610"
     CDS             498..1874
                     /locus_tag="VE170_12610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HYJ16328.1"
                     /translation="MDKIVSLCKRRGFIFQSSEIYGGTGSCWDYGPLGVELKNNIKRA
                     WWRDSVQSRGDMVGLDASILMHPRVWKASGHLDHFADPMVDCKVCKGRFRADKLEDAR
                     CPQKPSKQPGQHDACQLTEPRMFNLMFKTFMGPVEDEAAVAYLRPETAQGIFVNFENV
                     QQSMRLKLPFGIAQIGKSFRNEITPGNFTFRSREFEQMEIEFFVMPGTDEIWYQRWLD
                     ERFAWYAKYGIRKENLRLREHDKDELAHYAKRTADIEYLFPMGWSELEGVANRADFDL
                     KQHAQFSGKTLEYFDEESKQKIVPFVIEPSAGADRGTLAFLVDAYREEEVKGEKRVVL
                     QFHPELAPIKIAVLPLLKKNPQIVETAHKLCADLRKRWHSVYDDTASIGRLYRRQDEV
                     GTPFCVTVDVQTVGDEQAAADGKVTIRDRDNMQQIRVPLASVNAVMEELMDGGWAEVH
                     RQHGVARG"
     gene            2074..3792
                     /locus_tag="VE170_12615"
     CDS             2074..3792
                     /locus_tag="VE170_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16329.1"
                     /translation="MLGVLSLIATDLAGIALPWLIKGGIDTALRAKEGSATLLTYPML
                     IVGAAALQGFFRFCWRINIHGFSRRCEADLRDVVFAHLQKLPLSYFQYHKIGDLMSRL
                     TNDIHAVRELMGFGSLAIVDALVVIVFSLTLMIAIDPWLTLWSMMAMPLIPVVVRYFG
                     RLIFRWSRATQDQVSDMSAYTLENFSGIRVVQAYAQEENQISGFNRISSEYREKTMWL
                     ATLWGIFWPLMQVHAGIAATIVLWLGGRQVLAGTMSLGEFVAFNGYLAMLTWPLMAIG
                     YTVNQYQRGTAALTRIAEVLDMPVGARYASAADSEPGQTVRGDIELRNLTFSYEASQA
                     AGLRDINLHIPAGKSCGIVGETGSGKSTLVNLMLRLYEPPENSILIDGLDIRRMPLRE
                     LEDAIGYVAQDIFLFSGSLRENILLGVEYGELARVEEASRIARLLPAIESFGAGFDTV
                     IGERGVRLSGGQKQRTALARALIKNPPILILDDAFSSVDVETEEEILRELRQFMRGRT
                     TLLISHRISTVRDADTIVYLRDGAIIERGNHEQLLARRGAYFNLYQRQSLERQVGSLM
                     LEDGQR"
     gene            3789..5606
                     /locus_tag="VE170_12620"
     CDS             3789..5606
                     /locus_tag="VE170_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16330.1"
                     /translation="MIGAQLPDEEILGKAYDARLMVRILRYLRPYWKLLAVAFGFLLL
                     QTGTQLLGPYITKIAIDRYIATRDIHGLDLMALAYLGVVVCGFVVLFIQTYTTEYIGQ
                     RAMHDLRLEIFSHLQKQDLAYFDRNAVGRLMTRTINDVETLNELFSTGVVGLMGDVSI
                     VFGIAAVMLWLDWQLALVCLAAFPVIVYLSRFYRRRAREVYRQSRLILGRLNAGLQEN
                     IAGVATVQAFGQEERMYRRFQEVNLNYRDVLLQSIRNNAVFFPAIELFSAISVGLLLW
                     YGGLQVLGNAVEAGVLVAFLQYIQRMYQPIRDLAEKYNIMQAAMASSERIFLLLDEAE
                     TIKNPPEPKRVDRFAGAVEFKNVQLSYRPGDPVLKNISFRVRPGEKVALVGATGGGKT
                     SIISALCRFYDVDRGQILVDGIDVREWNKQELRRHLGLVLQDVFLFSGDIATNITLGD
                     PRISEQRMIDAAARAQITPFIDKLPRGFHEEVQERGSTLSQGQRQLLSFARALAFDPK
                     ILILDEATSSVDTRTEGLIQEALTELLQNRTALIVAHRLSTIKHADRILVIHKGEIRE
                     EGSHDELLARGGLYARLYELQYRFQERDTAVVAATAAPV"
     gene            complement(5627..6844)
                     /locus_tag="VE170_12625"
     CDS             complement(5627..6844)
                     /locus_tag="VE170_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYJ16331.1"
                     /translation="MNSSRRAFLQSAGLGFLALGLPPAFLVRAAQAQSAKNKSLVVVF
                     QRGAMDGLNVVIPFKDAAYYQLRPSIAVRQPGSNDESAIDLDGFYALHPALAPLKNIY
                     DKGHLGFIHAAGSPDNTRSHFDAQDYMEIGTPGIKSTADGWLNRALLDNKKNPSHFRG
                     IAVTAQLPRMLSGPTPALTLSSIEEFRFRNEALGKALAKLYANTQDRELRFGAENLFG
                     AMTELKRLDSKIPVSGERYPAGRFGVGLAQIARLIKADVGLEVAFTEIGGWDTHANEG
                     GATGQLANRLAELADGLAAFYRDLGDRMDDVVLLTMSEFGRTARENGNRGTDHGHANV
                     MFAMGGHVRGGKVYGRWPGLAPEALNEGRDLALTTDFRAVCGEVLTRHLGQKELTKIF
                     PGFSANTKLGFVT"
     gene            complement(6847..8283)
                     /locus_tag="VE170_12630"
     CDS             complement(6847..8283)
                     /locus_tag="VE170_12630"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HYJ16332.1"
                     /translation="MNAPLSQEQKIVHFLNRTSFGPTRNDIQRATRIGIRGYLDEQLT
                     PERIADNGADEKVAALKTMRLNSRELIELYPPPRVQRRQAQQANQPAGMTMESMEGPR
                     VVILELQQAKLLRALYSNRQLLEIMVDFWSNHFNIYIAKGANRWLTTAYDRDTIRPLA
                     LGKFKDLLLATAKSPAMLFYLDNWLSADPDSRGAQLGGPNNRRRGLNENYARELMELH
                     TLGVDGGYTQKDVQEVARCFTGWTITQPQGQGVFRFEPRIHDYGEKTVLGQRIPRGGG
                     MDDGLRVIDILVKHPSTAKFIATKLARRLVADEPPLGIVNKATDAFRQSDGDIPTVLR
                     AIVDTPEFFAGELYHAKIKNPLEFVASALRVTGSETQVTPQLLRYLGRMGEPLFLAQA
                     PTGYADIAASWISADMLLTRMNFVADLVANRLPGAKVPTATLNNRDELISRIAPNSLS
                     PATQAALAETEGHQAIALLLAAPEFQRR"
     gene            complement(8287..8904)
                     /locus_tag="VE170_12635"
     CDS             complement(8287..8904)
                     /locus_tag="VE170_12635"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HYJ16333.1"
                     /translation="MKALGKAAIVAGIAACLYAAPARSQIAQPGLTIAQNEQESRERW
                     QKMSPGEKQEMRERYQRWKDLDPKDKAELQDKFDNWRKLPPEEKARARRNFERFQKLK
                     PEDRERLQKRWEEYRKLSPERREEIKRRMQTLRDLPPERRQELRQKLLERREKVREHF
                     EKMPLDERQELREQLRERKKHGKFEDERRFREKRRENSPGGRDRE"
     gene            complement(8901..9152)
                     /locus_tag="VE170_12640"
     CDS             complement(8901..9152)
                     /locus_tag="VE170_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16334.1"
                     /translation="MNLRNPLPLLLLLIAVPGLFAATREAELPAKEMLKMMEFLREME
                     MVKHMEMMRELDGAESLDETGKTRVAQKPPAAKKKETSK"
     gene            complement(9162..9680)
                     /locus_tag="VE170_12645"
     CDS             complement(9162..9680)
                     /locus_tag="VE170_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYJ16335.1"
                     /translation="MTERINPAASACADLEADLVLLHYGDLDGVERARLQAHAATCAG
                     CSDYLHELATLLPLTVTTDTPPQEFWMNYNRELRHKIDAAVENKSWWHNFTAIFKPGY
                     LPAVASAALVVLALSFTLGGGLWSGKNGTPDDEMTEALPVAEHLEFFRAMDVLDELDL
                     LEVMGSTASDAA"
     gene            complement(9689..10354)
                     /locus_tag="VE170_12650"
     CDS             complement(9689..10354)
                     /locus_tag="VE170_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ16336.1"
                     /translation="MALLQAAQELEFFRRPLHSNANTRESTKGRTSQLANSGDEELVA
                     LAQQGDRRAFEELIERHKQKAYNIAFGFARDREDAKDLSQEAFLKAFTYLKNFDGRSS
                     FYTWFYRIVVNVCLDYKRRAKRTYAGEFDESIESQMESSHTPARPLAPEQHVLAGQIS
                     GKVDAALQKLPAKQRMAFILKNNQGLSIKEIAATMETAEGTVKVHLHRAVTTLRQSLA
                     ELV"
     gene            complement(10368..10763)
                     /locus_tag="VE170_12655"
     CDS             complement(10368..10763)
                     /locus_tag="VE170_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16337.1"
                     /translation="MKTKSLMGLGLFVAALGLANSPAIAAEGTVTPAEKAELKQDRKE
                     IRRDRREVKGDRRELRRDRKELRRDRRELRRDLKNGASKEEIAQDRNEIAQGKAEVKG
                     DRKELKQGRIERHQDRKELRTDRREAVKN"
     gene            11361..11630
                     /locus_tag="VE170_12660"
     CDS             11361..11630
                     /locus_tag="VE170_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16338.1"
                     /translation="MVSRITCAALLLFVFFLPLHFHFASSSQLNSQCSCLHGARAQLG
                     LHDDTPITTAPTSTFAIPLTAQSDEWNFATPQGPFVRGPPLSLPI"
     gene            11798..12967
                     /locus_tag="VE170_12665"
     CDS             11798..12967
                     /locus_tag="VE170_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16339.1"
                     /translation="MAQDSELESLRSLVKGMEQQLQKALQRIDQLENERGITATRVEQ
                     VEKSARARQSAPSALNPAIGMAIDATAEQRSKAGGTFNFRSAEIGIAASIDPYARGYA
                     YFTGSGEGVEVEEAAMVTTSLPWNLQARGGRFFADFGRLAKFHPHEYAFVNTPLSLER
                     IVGGESKADGAELSYLFPTPFFLRGTVGGYNKLGAENERLDDSKSRSWSRFTYLGRLN
                     TYFDLTDNHSVELGTSVAFTPSSRIPDVAQGGSRTLSGLDLTYRYQPLDSTLYQGLSF
                     GAELFGNSERLQRASGVRRMFAPGGYAYAEAKLNREWSAGFLYDNAPSLVSPGKKTQS
                     YSPFLTWNLSEFNRLRFQYTYGNDNVNESKADGGNQFFLQWTTTLGAHSHGFANR"
     gene            12996..13919
                     /locus_tag="VE170_12670"
     CDS             12996..13919
                     /locus_tag="VE170_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HYJ16340.1"
                     /translation="MQVKRRSFVCVLFLVVLWSAGGVAQAQSIRVVTTIPDLADITKQ
                     IGKELVEVESLTRGVEFMHAVPVKPSFVPKLNRANILVAMGLDLEISWLPALLEPASN
                     PKIMPGQPGHVDCSAGITVLDVPRSVDRAEGDVHPKGNPHYNLDPFNGKVIARNIADG
                     LGRNFPQHKAVFEKNLAAYSAELEKAVARWQTLAAPLKGTKFVAYHSDWAYFASRFGL
                     QQIGSIELKPGIEPTPNHLVALVQKMQQEKVQVIIYGPQSDRYPKQLAGQTGAAVVRL
                     QSTGGATAETDSYIKFIDYNVRSLLAALKKS"
     gene            13969..14649
                     /locus_tag="VE170_12675"
     CDS             13969..14649
                     /locus_tag="VE170_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HYJ16341.1"
                     /translation="MSNELPLVSLRASTLGYDGVAVLQAVDLDIRAGELIAMAGPNGS
                     GKTTIFRTILGFLPVISGVLVRNCMLSEFGYVPQSSALDSTFPITAREVVAMGGFRRL
                     KPYLGLPGREKRRLHDVLEQVGLGHLAGRSFFSLSGGQRQRMLIARALMVGPKVLILD
                     EPLSGVDPESQKAISDLLLLLNRDEGKAIFFSSHDLRMVRSVTDKILRVDGGKVVWEE
                     GSVAQHPW"
     gene            14643..16085
                     /locus_tag="VE170_12680"
     CDS             14643..16085
                     /locus_tag="VE170_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYJ16342.1"
                     /translation="MVSFWEIFSPSFLLFPALLGSVILAFVCPLVGAHLMLRRRVFLG
                     LTLPQIAACGVACTFWLQHALGFAHGSGGERLLATVGSLVFTLAGMGLLAYLDRRATG
                     SPEGRLAAAYALASAVTILFIVFNPAGELEILNLLKGEVISLSKEELKLLAIVYGVVY
                     VGLVLFRREFLLNSFDRDLSFLLTGGSAQWSFWLYVLCGFSIAIGVIMAGPLMVFGFM
                     VLPPLAARALARGMSSFLSLSSLIGIFIAVLGFYLSVRLDLPLGPTDVAFACVVIFAV
                     YAVQSLLRRGRIAGLMVFIVTLCAGCGAQPPEAPFDPSTLGQGPVWLASVGNRTGSDL
                     RLPGTNPLRSLGEMAGKLSPESRPTVMDLLRGHLQRELQDRRVNVGLPEERDARLQSF
                     PFDGVRAAANARTANLSGWLWLWEIRRWHTDGRSPLRTVVEMQLVRIATGAVSWQRRI
                     VKTVPLSGAARLDETSADAIRDILREVFGA"
     gene            16192..17364
                     /locus_tag="VE170_12685"
     CDS             16192..17364
                     /locus_tag="VE170_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16343.1"
                     /translation="MASILIKNIGTLVTGKLASPLRRADSIFIDDGLVKAIGNGLSQA
                     ADQTIDAHGITAIPGLIDSHSHPSIGEYTPAQNTLGWITNYMHGGVTALISAGELHLP
                     GLPLPPDARTALSVAIVTKKCYDNLRPSGVKVHAGTLLLVPGLTEKDFDEIQALGIKL
                     VKFIFYDYSLLPNGEANRYVDWAHARGIKVKIHSGGVSRSGVSQVAGFDMVQKIKPDI
                     IGHITGGPIPMPEADMLRIANETECFMEICTSGNYRLALSLARAVKANGRYDRVLIGT
                     DTPGGTGIVPRGILREIAFISSVGEIAPEKTICMATGNVGRAHDLNLGILEEGRPADV
                     VLMHKITGSVSKDALDSFAKGDLPGISYVIIDGVVQVAGRSQQTPPPEVLANQSCC"
     gene            17377..18357
                     /locus_tag="VE170_12690"
     CDS             17377..18357
                     /locus_tag="VE170_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16344.1"
                     /translation="MSAYEFGVGLFPTEPLEKMIQLAKVSEEVGFSHIWVGDSHLIWR
                     EAYINMTAMMLNTTKVKLGTGVTNPLTRHPSVSASGYATLEEYSPGRMIVGIGLGDSS
                     VETMGMKPSTLANFEKSLEQMRQILDGKEAQLPTGKIHLLHPTKTRVPIYIAASGPKM
                     LELSGRIADGIIVLVGVADDYIAHAKEKIAAGAKAAGRKLADLNLVLWVPCAVSETAN
                     AKDAVKAHVARVVAHPLPYVLDPNEQKVLQEIRKTYDYYHHMDQQANHADVIPDWLVE
                     KFAIAGTVAQCKTQIERIKKTGVQQIAIIPYSPPGGSREETIRGFAKAMN"
     gene            complement(18455..>18638)
                     /locus_tag="VE170_12695"
     CDS             complement(18455..>18638)
                     /locus_tag="VE170_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16345.1"
                     /translation="LSAFCNWTPLGHILSPTADRFGYLIVTTDILRQQHTCQRKTQVF
                     NALQGFTAPAPYENAL"
BASE COUNT         3606 a         5269 c         5534 g         4229 t
ORIGIN      
        1 cgacgactac gacgtggaga tcgtcgaagc gcatcatcgt tttaaaaaag acgctcccag
       61 tggtacggct ctagcgctcg gccgcacggt cgctaaggcg ctcaatcgtg atttggacaa
      121 agtcggcatc aacggccgca agggcatcgt cggcgcgcgc ggtaaaaaag aaatcgccct
      181 actgtcggtc cgcgccggcg acatcatcgg cgaacatacg gtcatttttg gcggtatcgg
      241 cgaaaggctt gaatttattc atcgcgccca cagccgcgac accttcgccc atggcgccat
      301 ccgcgcggcc caatggttag cgcggagaaa gcccggattg tacggcatgc aggatatgct
      361 gggctggtag ctcgacggcg gtgcacgatc gcgccgtatt aaggccacga accgattttt
      421 gctgcttttt gactctcttg gttgcatccc ttagagtaat gccattcatc gcaagatagg
      481 attttttaat ggttgcaatg gacaagatcg tgtcgctgtg caagcggcgc ggctttattt
      541 ttcaatcgag cgagatttat ggcggcaccg ggtcctgttg ggactatggg ccgctcgggg
      601 tcgagcttaa gaacaatatc aagcgcgctt ggtggcgcga ctcggtgcag agccgcggcg
      661 atatggtcgg actcgatgcg tcgattctca tgcatccgcg ggtgtggaag gcgagcggtc
      721 atttggatca ttttgccgat ccgatggtcg actgcaaggt gtgcaaagga cgctttcgcg
      781 ccgacaagct ggaagacgcg cgttgtccgc aaaagccgtc gaaacagccc ggacaacatg
      841 atgcttgcca gctcaccgaa ccgcggatgt tcaatctaat gttcaagact ttcatggggc
      901 cggtggagga cgaagccgct gtcgcttatc tccggccgga gaccgcccag gggattttcg
      961 tcaacttcga aaatgtccag cagtccatgc gtttgaaact gcccttcggc atcgcccaga
     1021 tcggcaagtc cttccgcaac gaaatcacgc cgggcaattt cacctttcgc agccgcgaat
     1081 tcgagcagat ggaaattgag tttttcgtca tgccgggcac cgacgagatt tggtatcagc
     1141 gctggctcga cgaacgcttc gcctggtatg cgaaatacgg cattcgcaag gaaaacctgc
     1201 ggctgcgcga gcatgacaaa gacgagctgg cgcattacgc caaacgcact gccgatatcg
     1261 aatatctttt cccgatgggt tggagcgagc tcgaaggagt cgccaaccgg gccgatttcg
     1321 acctgaagca gcatgcccaa ttcagcggca agaccctgga atactttgac gaagagagta
     1381 agcaaaaaat tgttcccttc gtcatcgaac cgtcggccgg cgccgatcgc ggcaccctgg
     1441 cattcctcgt cgatgcttat cgcgaggaag aggtgaaggg ggaaaagcgc gtggtgctgc
     1501 agtttcatcc cgagctggcg ccgatcaaga tcgcagtgtt gccgctcttg aagaagaatc
     1561 cacagatcgt cgagacggcg cataagctgt gcgccgacct gcgcaagcgc tggcattcgg
     1621 tgtacgacga taccgcctcc atcggccgac tctaccgccg ccaagacgaa gtcggcacac
     1681 cgttctgtgt caccgtcgac gtccaaaccg tcggcgatga gcaggccgcc gccgatggca
     1741 aagtaacgat tcgcgaccgc gacaacatgc aacagatacg cgtgccgctt gcgtccgtga
     1801 acgctgtcat ggaagagctg atggacggcg gctgggcgga ggttcatcgc caacatggcg
     1861 ttgcgcgggg ttgaaaccta gtgcggtgcg gcggaaccac acggaaaaat tgccgcagcg
     1921 aacgcaaagg ccgcacaaag aatcgcggat ttgtcagggc gggtttgaaa cccgccctcg
     1981 tcatatgcac gcagccccga accaaaggat agaaagattt ttcgttacta ccaccgggtc
     2041 aaatttctta ttgaccgcta tcgccggctg ctcctgcttg gcgtcctttc cctgatcgcc
     2101 accgatctcg ccggcatcgc cttgccttgg ttgatcaagg gcggcatcga cacggcgttg
     2161 cgggccaagg aaggcagcgc cacgctgctc acctacccga tgctgatcgt cggcgcggcc
     2221 gcgctgcaag gattctttcg cttctgctgg cgcatcaaca ttcatggttt ctcgcgccgc
     2281 tgcgaggccg atctgcgcga tgtcgtgttc gcccatctgc agaagctgcc gctcagctat
     2341 tttcagtacc acaagattgg cgacctgatg tcgcgcctga ccaacgatat ccatgccgtg
     2401 cgcgaactca tgggtttcgg ctcgctggca attgtcgatg cgctggtggt gattgttttc
     2461 agcctaacgc tgatgatcgc catcgaccct tggctgacct tatggtcgat gatggcgatg
     2521 cccttgatac cggtggtggt gcgctatttc ggccggctta tttttcgctg gtcgcgggcg
     2581 acccaggacc aagtgagtga catgagcgcc tacacgctgg agaatttttc cggcatccgc
     2641 gtggttcaag cctacgccca agaagagaac caaatcagcg gtttcaatcg gataagctcc
     2701 gagtaccggg aaaagaccat gtggctggcg acgctgtggg ggatattctg gccgctcatg
     2761 caggtccacg ccggcatcgc cgcgaccatt gtgctctggc tcggcggccg gcaagtattg
     2821 gcaggcacca tgagcctagg tgaatttgtc gctttcaacg gctatctggc gatgctgacc
     2881 tggccgctca tggcgattgg ctacaccgtt aatcaatatc aacgcggcac cgcggcgttg
     2941 acgcgcatcg ccgaagtgct ggatatgccg gtcggtgccc gctatgccag cgccgccgat
     3001 tccgagccgg gacagaccgt tcgtggcgac atcgagctgc gcaacttgac tttctcctac
     3061 gaagctagtc aagcggctgg actgcgcgat atcaatttgc atattccggc cggcaagagt
     3121 tgcggcattg tcggcgagac cggttcgggc aagagcacct tggtcaatct catgctgcgg
     3181 ctctatgagc cacctgagaa ttcgattctt atcgacggtc tcgatattcg ccggatgccg
     3241 ttacgcgagc tggaggacgc catcggctat gtcgcccagg atatctttct attctccgga
     3301 tcgttgcgcg agaatatttt gctcggggtc gagtacggcg aattggcgcg ggtcgaggaa
     3361 gcgtcgagaa tcgcccggct gttgccggcg atcgaatctt ttggcgccgg tttcgatacc
     3421 gtgatcggcg agcgcggcgt gcgcctatcg ggcggacaga agcagcgcac cgcactggcg
     3481 cgggcgctga tcaaaaatcc gccgattctg attctcgacg acgccttttc cagcgtcgat
     3541 gttgaaaccg aagaagagat tcttcgcgag ctcagacaat ttatgcgcgg ccgcacgacg
     3601 ctgttgattt cgcaccggat ctcgacggtg cgcgacgccg acacaatcgt ctacctgcgt
     3661 gacggcgcga ttatcgagcg cggcaaccac gagcagctct tggcgcgccg cggcgcttat
     3721 ttcaatcttt atcagcgtca gagtttagaa cggcaagtcg gtagtctgat gcttgaggac
     3781 gggcagcgat gatcggcgcc caacttcccg atgaggagat cctcggtaag gcctacgacg
     3841 cgcgcttgat ggtgcgcatc ctgcgttatc tgcggccata ctggaagctg ctcgccgtgg
     3901 cgtttggctt tttgctgctg cagaccggca cgcaactact cgggccttat atcaccaaga
     3961 tcgccatcga tcggtatatt gccactcggg atattcacgg cctcgatttg atggcgctgg
     4021 cctatctcgg cgtggtcgtg tgcggctttg tcgtattgtt cattcagacc tacaccaccg
     4081 agtatatcgg ccagcgcgcc atgcatgatt tgcgcctgga aattttcagc catttacaaa
     4141 agcaggatct cgcttatttc gaccgcaacg ccgtcggccg gctgatgacc cggacgatca
     4201 acgatgtcga gacgctcaac gaactgttca gcaccggagt cgtcggtctg atgggcgatg
     4261 tctcgatcgt tttcggcatc gcggcggtga tgctttggct cgattggcaa ctagcgctgg
     4321 tctgtctcgc ggcgtttccg gtgattgtct atttgagtcg gttttatcgc cggcgcgccc
     4381 gcgaggttta tcgccagagc cggctgattc tcggccggct caatgccggc ttgcaggaaa
     4441 acatcgccgg cgtggcgacg gtgcaggcgt tcggccaaga agagcgaatg taccggcgct
     4501 ttcaagaggt caatctcaac tatcgcgatg ttctgctgca gagcattcgt aacaacgcag
     4561 tgttctttcc ggcgatcgaa ctgttcagcg ccatctcggt gggactgctg ctgtggtacg
     4621 gcggtttgca ggtgctcggc aatgccgtcg aagccggtgt gctggtggcg tttctccagt
     4681 acattcagcg catgtaccaa ccgatccgcg acctggcgga gaagtataat atcatgcaag
     4741 ccgccatggc ctcgtcggaa cggatttttc tcttgctcga tgaggctgaa accatcaaga
     4801 atccgcctga gcccaagcgc gtcgacaggt tcgccggcgc ggtcgaattc aaaaacgtcc
     4861 agctgtctta ccggcctggc gatccggtgc tgaaaaatat ctcgtttcgc gtgcggcccg
     4921 gcgaaaaagt cgcgctggtg ggcgccaccg gcggcggcaa gacctcgatc atctccgcgc
     4981 tgtgccgctt ctacgatgtc gacaggggac agattctcgt cgatggcatc gatgtccggg
     5041 agtggaacaa gcaggagctg cgccgccatc taggtttggt gttgcaggat gtgtttctct
     5101 tttccggtga catcgccacc aacatcactc tcggcgaccc gcgtattagc gaacagcgca
     5161 tgatcgacgc ggcggcgcgc gcgcagatca caccgttcat cgataagctg cccaggggtt
     5221 tccatgaaga agtccaagag cgtggttcga ctttgtcgca ggggcagcgc cagttgttgt
     5281 ctttcgccag ggccctcgcc ttcgacccga aaattctcat tctcgacgag gcgaccagct
     5341 cggtggacac ccggaccgaa gggttgatcc aagaagcgct gaccgaactg ctgcaaaatc
     5401 gcaccgcgct gatcgtcgcg caccgattgt cgacgatcaa gcacgctgac cggattctcg
     5461 tcatccacaa aggcgaaatc agggaagagg gcagtcacga tgagctgctg gcccgcggcg
     5521 gtttgtatgc gaggctgtac gaattgcaat atcggtttca ggaacgggac acggcggtgg
     5581 ttgccgcaac cgccgcgccg gtgtgagcaa cggccaacac tgcaatttag gtgacgaatc
     5641 ccaattttgt attcgcgcta aacccgggga aaattttcgt cagctctttc tggccaagat
     5701 ggcgggtcag cacttcgcca cagacagcgc ggaagtccgt ggtgagggcg aggtctcgcc
     5761 cttcgttaag cgcttccggc gcaaggccgg gccagcggcc gtagactttg ccgccgcgca
     5821 cgtgtccgcc catggcgaac atgacgttgg cgtgaccatg atcggtgccg cggttgccgt
     5881 tctcccgggc ggtccggccg aattctgaca tggttagaag cacgacatca tccatacggt
     5941 cgccgagatc gcggtaaaat gccgccagcc cgtcggccaa ttctgccagc cggttagcca
     6001 gttgaccggt ggcgccgcct tcgttggcat gggtgtccca gccgccgatc tcggtgaagg
     6061 cgacttccaa accgacgtcg gctttgatca accgggcgat ttgcgccaga ccgacgccaa
     6121 aacggccggc cgggtagcgt tcgcccgaga cagggatttt gctgtcgaga cgtttgagct
     6181 cggtcatggc gccaaaaaga ttctcagcgc cgaagcggag ctcgcggtcc tgggtgttgg
     6241 cgtagagttt cgccaaggct ttgccgaggg cttcgttgcg gaagcgaaac tcctcgatcg
     6301 acgacagcgt tagcgccggg gtagggccgg acaacatgcg cggcagctgc gcggtgacgg
     6361 cgatgccgcg aaagtgcgat ggattttttt tattgtccag caaagcgcgg ttgagccagc
     6421 cgtccgccgt gcttttgatg cccggcgtac cgatctccat gtaatcttgg gcatcgaaat
     6481 gggagcgggt gttatccggc gaaccggcgg catgaataaa gcccagatga cctttgtcgt
     6541 aaatgttttt caacggcgcc aatgccggat ggagtgcgta aaagccgtcg agatcgatgg
     6601 cgctttcatc gttcgatccc ggctggcgca cggcgatgct cgggcgcagt tggtaatagg
     6661 ccgcatcttt gaacggaatg accacgttca agccatccat cgcgccgcgc tgaaatacca
     6721 cgaccaggga tttgtttttt gccgactgcg cttgcgcggc gcggacgaga aacgccggtg
     6781 gtaaaccgag ggcgagaaag cccaaaccgg cggactgtag aaaggcgcgg cgcgacgagt
     6841 tcatctctac ctccgttgaa attccggcgc tgccaatagc agcgcgatgg cttgatggcc
     6901 ttcggtttcc gccaacgctg cttgagtggc cggcgatagc gagttcggcg cgatgcggct
     6961 aatgagctcg tcccgattgt tcagcgttgc tgtcggtacc tttgctcccg gcaaacggtt
     7021 tgcgaccagg tcagcaacga agttcatgcg cgtgagcaac atatcggcgc tgatccaaga
     7081 ggccgcgata tcggcatagc cggtgggcgc ttgggctagg aataacggtt cgcccatgcg
     7141 ccccaagtag cgcagcagtt ggggcgtcac ctgggtttcg ctgccggtga cccgcagggc
     7201 gctggcgacg aactctaaag gatttttaat cttggcgtga taaagttcgc cggcaaaaaa
     7261 ctccggggtg tcgacgatgg cgcgcagtac cgtcgggata tcgccatcac tctggcgaaa
     7321 cgcgtcggtg gctttgttga cgatccccag cggcggctcg tcggcgacca atcggcgagc
     7381 cagcttggtc gcgataaact tcgccgtcga agggtgtttg accagtatat cgatgactcg
     7441 caaaccgtca tccataccgc cgccgcgcgg aatacgctgg ccaaggacgg ttttttcacc
     7501 gtagtcatga atgcgcggct cgaaacgaaa cacgccttgg ccctggggct gggtgatcgt
     7561 ccagccggta aaacagcggg ccacttcctg aacatctttt tgcgtgtagc cgccgtcgac
     7621 gcccaaggta tgcagctcca ttagctcgcg cgcgtagttc tcgttcaagc cgcggcgccg
     7681 attgttcggc ccgccgagtt gagcgccgcg ggaatccgga tcggcgctca gccagttgtc
     7741 caaataaaac aacatcgccg ggcttttggc cgttgccagc aacagatctt taaacttgcc
     7801 taaagccagc gggcggatcg tatcgcggtc gtaggcggtg gtcagccagc ggttggcgcc
     7861 tttagcaatg tagatgttga aatggttgct ccagaaatcg accatgattt ccaagagttg
     7921 gcggttgctg tacagcgccc gcaaaagttt cgcctgttgc agttcgagga tgacgacgcg
     7981 cggcccctcc atggactcca tggtcatgcc ggccggttga ttggcctgct gggcttgccg
     8041 tcgctgcact cttggtggtg gatagagttc gatcagttca cggctgttaa gccgcatggt
     8101 tttcaacgcc gcgacttttt cgtcggcgcc attgtcggct atgcgttccg gtgtgagctg
     8161 ttcgtcaaga tagccgcgga tgccgatgcg tgtcgctctt tgaatgtcgt tcctggttgg
     8221 gccgaagctg gtacgattta agaagtgaac tatcttctgt tcttggctga ggggtgcgtt
     8281 catgggctac tccctgtctc gaccgccggg cgaattctcg cgccgcttct cgcgaaagcg
     8341 ccgctcgtct tcaaacttgc cgtgcttttt gcgctcgcgc aattgttcac gcaactcctg
     8401 ccgttcatca agcggcatct tttcgaaatg ctcgcgcacc ttctcgcgcc gttcaaggag
     8461 cttttggcgc aattcctggc gtctttccgg cggcaggtcg cgcagcgtct gcatgcgccg
     8521 cttgatttcc tcccgtcgtt cgggcgacag cttgcgatac tcttcccagc gtttttgtaa
     8581 tcgctcgcga tcctcaggct tcagtttctg aaagcgctcg aaattgcgcc gcgccctagc
     8641 cttctcttcg ggtggcagct tgcgccagtt gtcgaacttg tcctgcagtt cggccttgtc
     8701 cttgggatca aggtctttcc agcgctggta gcgctcgcgc atctcctgtt tttcccccgg
     8761 tgacattttc tgccagcgct cgcgggactc ctgttcgttc tgggcgatcg tcaaacccgg
     8821 ttgcgcgatt tggctgcgtg ccggcgcggc gtacaagcac gccgcaatgc cggcgacgat
     8881 ggccgctttg cccaatgcct tcacttggag gtctccttct ttttcgcggc tggcggtttt
     8941 tgcgccacac gggtcttgcc ggtttcgtcc aatgattctg cgccatccag ttcccgcatc
     9001 atttccatgt gcttgaccat ttccatttcg cgcaggaact ccatcatctt gagcatctct
     9061 ttagcgggca gctccgcttc cctggtcgcg gcgaataatc cgggcacggc gatcagtagt
     9121 aatagcagcg gcaacgggtt gcgaagattc acgtaaaggg ctcaagccgc gtcgctggcc
     9181 gtgctgccca tgacttcgag cagatccaat tcatccaaaa catccatggc gcgaaaaaat
     9241 tccaagtgct ccgccaccgg cagcgcttcg gtcatctcgt cgtccggcgt gccgtttttg
     9301 ccagaccaca ggccgccacc gagagtgaaa ctcagcgcca agaccaccag cgccgcgctg
     9361 gcgaccgcgg gcagatagcc gggtttgaaa attgccgtga aattgtgcca ccaagatttg
     9421 ttttcgaccg cggcgtcgat cttgtggcgc agttcacggt tatagttcat ccagaactct
     9481 tgcgggggtg tgtcggttgt aaccgtcagc ggcaacagcg ttgcgagttc gtgcaagtag
     9541 tccgagcagc ccgcgcaagt ggcggcatgg gcttggagcc gcgcgcgttc aacgccgtcc
     9601 aaatcgccat agtgaagcag cacgaggtcg gcttccaagt ctgcgcaggc gcttgctgcc
     9661 gggttgattc gctccgtcat aattcctcct agaccaattc cgccaagctt tgccgtagcg
     9721 tggtgacggc gcggtgcaaa tgcactttca cggtgccctc ggcagtttcc atggtcgcgg
     9781 cgatttcctt aatcgaaagt ccttgattgt tcttcaaaat aaatgccatg cgttgtttcg
     9841 ccggcagttt ttgcagggcg gcgtcgactt tgcccgagat ctggccggcc agcacatgct
     9901 gctccggcgc tagaggccga gccggagtat gggacgattc catttggctc tcgatgctct
     9961 cgtcaaactc gccggcgtaa gtgcgcttgg cgcgccgctt gtaatcgaga cagacgttga
    10021 cgacgatgcg ataaaaccaa gtataaaaac tcgaacgtcc gtcgaagttc ttcaagtaag
    10081 taaacgcctt caagaacgcc tcttgtgaca gatccttggc atcctcgcga tcgcgggcga
    10141 aaccgaaggc gatattgtag gccttctgtt tatggcgctc gatcagttct tcgaacgccc
    10201 ggcggtcgcc ctgttgggcc agcgccacca actcctcatc cccagagttc gccaattggc
    10261 tggtcctgcc tttcgtcgat tcccgcgtgt tcgcattact gtggaggggg cggcggaaaa
    10321 attcaagttc ttgcgccgct tgaaggagtg ccatgatcga ctcccggtta gttcttgacg
    10381 gcttcgcggc ggtcggtccg cagctctttg cggtcctggt gccgttcgat gcggccttgt
    10441 ttcaattcct tgcgatcgcc tttgacctcc gccttgccct gcgcgatctc gttgcggtct
    10501 tgggcgattt cttctttgct cgcgccattc ttgaggtctc ggcgtaattc acgacggtcg
    10561 cggcgcagct ctttgcggtc cctgcgcaac tctcggcggt cgcccttgac ctccctgcgg
    10621 tcgcggcgga tctccttgcg gtcctgtttc aactcggctt tctcggccgg ggtgaccgtg
    10681 ccttcggcgg cgattgctgg cgaatttgcc agccccaagg cggcgacgaa cagtcccagg
    10741 cccatgagcg atttagtttt catgtcgaat acctcgtagg ctagttttgg tttttctagg
    10801 ggttagacga cgctaagggg gatcggttta ccggctctac tggaaaacct caataacggt
    10861 ttgcctcgct gcaaagtcgg cctgttcgcg gctgtttcgc ctgacggttc ggctgaatcg
    10921 gtagctggct ggggcggata cggcaaaagg cgtgaatttc cggggagtcg ccgagcctta
    10981 ctcccgcgtc agggtggcta ggtacgattt ggccaagcat aatcgctcgg cgatcaaact
    11041 cggcaatatg cttcgggcga atcgagggag aacggtgtga atatcctcag gtcactgcta
    11101 ttgtcactct tccgtgtgcc tgcatacccc gccctcaaga ggacgcgcaa tcaacaatat
    11161 cgtagcgcat tatcggagga tctgaatccg tcgaatgtca taatagcagc ggactgtatc
    11221 gagcccctag atatgccggt gccgcttgcg cgattccggt aataaggact gccggtgtct
    11281 gcgagcgttt taaattgaca aggtgaatgc ctttgattag gttaactccc atgggggtct
    11341 cgggagtcag gacagcaaaa atggtgagcc gtatcacctg tgcggcgcta ttgctgtttg
    11401 ttttcttttt gccgctgcat ttccattttg cctcgtcgtc acagctcaat agccagtgca
    11461 gttgtctgca tggcgctcgc gcgcagttgg gcttacacga cgatacgccg atcacaacag
    11521 ctccgacctc tacctttgcg atcccactta cagcacaaag cgacgagtgg aatttcgcca
    11581 cgccgcaggg tccatttgtc cgcggaccgc ctctctcact gccaatctag ttcatcaaac
    11641 gttggccata ttggtttttc ctccctcggg ttcgtgcgag cggagggaat ttcgttttct
    11701 cgagggaggt ctcgtcatgg tttcatccgc tttgcggaca ccatcactgg cggtgtccgc
    11761 cgtattttcc gttttattat tcatttcgga gcggggcttg gctcaagact ccgagctcga
    11821 atcgttgcgg tcgctggtca agggcatgga gcagcaactg caaaaagcgt tgcagcgcat
    11881 cgatcagctg gaaaatgagc ggggcatcac agccacgcgg gtggagcagg tcgagaagtc
    11941 ggccagagcg agacaaagcg cgccgtcggc gcttaatccg gccattggca tggcgatcga
    12001 tgccaccgcc gagcaacgca gcaaagccgg cgggacattt aatttccgct cggcggagat
    12061 cggcatcgcc gcctccatcg atccctatgc gcgcggctac gcttatttta ccggcagtgg
    12121 cgaaggcgtc gaggtcgaag aggcggcgat ggtgacgacc tctttgccgt ggaatttaca
    12181 agcccgcggc gggcggtttt tcgccgactt cggccgcttg gccaagttcc atccccatga
    12241 atatgcgttt gtaaatactc ctttgtcttt ggagcgtatc gtcggcggtg agtccaaagc
    12301 cgacggcgct gagctgagct atctttttcc gacaccgttt ttcttgcgcg gcaccgttgg
    12361 cggttacaat aagctcggcg cggaaaacga acggttggat gacagcaagt cccgctcgtg
    12421 gagccggttc acttacctcg gtcggctcaa tacctatttt gatctgaccg ataatcatag
    12481 cgtcgaattg ggaacaagcg ttgcttttac tccttcgagt cgcattcccg atgttgcgca
    12541 aggcggtagc cggacactca gtggccttga cctgacctat cgttaccaac cgctcgatag
    12601 cacgctgtat caaggcttgt ccttcggcgc cgaactgttc ggcaacagcg agcggctcca
    12661 gcgcgcctca ggggtccggc gcatgtttgc tcccggcggg tacgcctacg ccgaggccaa
    12721 gctcaaccgc gaatggtcgg caggtttttt gtacgataac gcgccgagct tggtgagccc
    12781 aggaaagaag acccagagct attcaccttt cttgacctgg aatctgagcg agtttaaccg
    12841 gctgcgcttc caatacacct acggcaacga caacgttaac gaatcgaagg ccgatggtgg
    12901 gaatcaattt tttctgcagt ggaccaccac gttgggcgcc cattcgcacg gcttcgccaa
    12961 ccgctaatgg tggagtcaat gaaggagata aagtcatgca agtgaaacga cgaagttttg
    13021 tctgcgtgtt gtttttggtt gtgctctgga gcgccggggg cgttgctcaa gcgcagtcga
    13081 ttcgtgtggt tacgactatt cccgatctgg cggatatcac caaacagatc ggtaaggaat
    13141 tggtcgaggt cgagagtctc acccgcggtg tcgagttcat gcacgcggtg ccggtcaagc
    13201 cgagtttcgt tcccaagctc aaccgcgcca atattcttgt ggcgatggga ttggacttgg
    13261 aaatttcttg gctgccggcg cttttagaac cggcgagtaa tccgaaaatc atgccgggcc
    13321 agccgggcca tgtcgattgc tcggcgggga ttaccgtgct ggacgtgccg agaagcgttg
    13381 accgcgccga aggtgatgtt catcccaagg gcaatccgca ttacaacctc gatccgttca
    13441 acggcaaagt gatagcgcgc aatatcgccg acggattggg gcgtaatttt ccgcaacata
    13501 aagctgtctt tgagaagaac ttagccgcct actcggccga gttggagaaa gcggttgcaa
    13561 gatggcaaac cctggcagcg ccactgaagg gaaccaagtt cgtcgcctat catagcgatt
    13621 gggcttattt cgccagccga ttcggtcttc agcagatcgg cagcatcgaa ctaaaacccg
    13681 gcatcgagcc gacgcccaat cacttggtcg cgctcgttca gaaaatgcag caggaaaaag
    13741 tccaagtgat catctatggg ccgcagagcg accgctaccc gaagcagctc gcaggtcaga
    13801 ccggcgctgc cgtggtgcga ttgcaaagca ccggcggcgc gacggcggaa accgacagct
    13861 acatcaagtt tatcgattac aatgtcagga gtttgctggc cgcgctcaaa aagagttagg
    13921 aaagatcctt aatgcagctt gaaaacgatc gttttgagga gagcttgcgt gagtaacgaa
    13981 ctccccttgg tttcgcttcg cgcttcaacg ctcggttatg acggcgtcgc ggtattgcaa
    14041 gccgtcgatt tggatatccg cgccggcgag cttattgcga tggccggacc caacggttcc
    14101 ggcaagacga caatcttcag gacgattctg gggtttcttc ccgtgatcag tggcgtgctc
    14161 gtgcgcaatt gcatgctcag cgagttcggc tatgtgccgc agagttctgc tctggattcg
    14221 acctttccga tcaccgcgcg cgaagtggtg gcaatgggcg gcttccgacg gctcaaaccc
    14281 tatctgggat tgccgggacg ggaaaaacgg cgcttgcacg acgtgctcga gcaggtcggg
    14341 ttaggtcatc tggcgggacg ctctttcttt tcgctgtcgg gcgggcagcg tcaacgcatg
    14401 ttgatcgccc gggcactcat ggtcggcccg aaggttttga ttctcgacga accgctgtcc
    14461 ggcgtcgacc cggaatcgca aaaggcgatc agcgatcttt tgctcctgct caatcgtgac
    14521 gaaggtaagg cgattttctt ttcgagccat gatttacgca tggtgcgcag cgtgaccgac
    14581 aagatcttgc gcgtcgacgg cggcaaagtg gtgtgggaag aaggatcggt ggcacaacat
    14641 ccatggtgag tttttgggaa attttcagtc cgtcgttttt gctttttccg gcgttgcttg
    14701 gcagcgtcat actcgcgttt gtttgtccgc ttgttggcgc gcacttgatg ctgcgccggc
    14761 gcgtgtttct cggtttgacg cttccccaga tcgccgcctg cggcgtcgcc tgcacttttt
    14821 ggctgcagca tgccttgggt tttgcccacg gttccggcgg cgaaaggctc ctggcgacag
    14881 tcggctcgtt agtcttcacg ctggcgggta tgggtttgct cgcctatctg gaccgccgcg
    14941 cgaccggttc gccggaagga cgtttggccg cggcttacgc gctggcgtcg gcagtgacta
    15001 ttctatttat tgttttcaac ccggcgggcg agttagagat tctcaatttg ctcaagggcg
    15061 aagtaatttc gttgtccaag gaggaattga aactgctcgc gatcgtctac ggcgtggttt
    15121 atgtcggtct ggttttgttt cgccgggaat ttttactcaa ctccttcgat cgcgatctgt
    15181 ctttcttgct taccgggggc agcgcccagt ggagcttttg gttgtacgtt ctgtgcggct
    15241 tcagcattgc cattggcgtc atcatggctg gaccgctcat ggtctttggt ttcatggtct
    15301 tgccgcccct ggcggcgcgc gccttggcgc gcggcatgag ttcgtttctg tcgctctcgt
    15361 cgttgatcgg tattttcatc gcggtcttag gcttctatct atccgtgcgc ctggatttgc
    15421 cgctgggacc gacggatgtg gcgtttgcct gcgtggtgat tttcgccgtc tatgccgtgc
    15481 aatcgctctt gcgccgcggc cggatcgccg gactaatggt attcattgtt accctgtgcg
    15541 ccggttgcgg cgcccagccg cctgaggcgc cgtttgaccc atcgacattg ggccaggggc
    15601 cggtgtggct cgcttcggtg ggcaaccgga cgggttcgga cctgcgttta cccggcacga
    15661 atccgctgcg ttctttgggt gagatggccg gtaaactgtc gccagagtcc cggcccacag
    15721 tgatggacct cctgcgtggt cacttgcagc gcgaacttca ggaccgacgg gttaacgtgg
    15781 gattgccgga agagcgcgat gcccgcctgc agtcgtttcc cttcgatggg gtgagggccg
    15841 cggccaatgc gcgcacggcc aacctctctg gctggttgtg gctgtgggag atccgccgtt
    15901 ggcataccga tggccgttcg ccgttgcgga cggtggtgga gatgcaattg gttcgcatcg
    15961 caaccggggc agtgagttgg caacgccgga ttgtcaaaac ggtgccgctc tccggtgccg
    16021 cgagattgga cgagacctcc gccgacgcga tcagggacat cctgcgcgaa gtctttgggg
    16081 cctaaaggcc ggtaatcttt atttcgcacg gcttctttgc gtctacgaaa gtttgtcacc
    16141 gggtttacac caaaaaacac tttgatatag tcccccgcca agtggtgaat tatggcttcg
    16201 atcctgatta agaacatcgg cacgttggtc acgggcaaat tggcaagccc gcttaggcgg
    16261 gcggactcga ttttcattga cgatggtctc gttaaggcga tcgggaacgg cctgtctcaa
    16321 gccgccgacc aaaccataga cgcccacggc atcacggcaa ttcccggttt gatcgattcc
    16381 cattcgcatc cctccatcgg tgagtacacg ccggcgcaga ataccctcgg ctggatcacc
    16441 aactatatgc acggcggcgt gaccgcgctg atctccgccg gtgagttgca tctgcccgga
    16501 ctgccgctcc cgccggacgc gcggacggcg ctgtcggtcg ccatcgtcac caagaaatgc
    16561 tacgacaacc tgcggccgtc cggggtgaaa gtccacgccg gtaccttgct cctggttccg
    16621 ggcttgacgg aaaaagactt cgatgagatt caagccctcg gtatcaagct ggtcaaattc
    16681 attttctacg actacagcct attgcccaat ggcgaagcca accgctacgt cgactgggcg
    16741 cacgcccgcg gcattaaggt caaaattcat tctggcggcg tatcgcgatc cggtgtgagc
    16801 caagtggccg gcttcgacat ggtgcaaaag atcaaaccag acatcatcgg tcatattacc
    16861 ggcgggccga tcccgatgcc cgaagcagac atgttgcgca ttgccaatga aaccgaatgt
    16921 ttcatggaga tctgtacgtc ggggaattac cggctggcgc tgagcctcgc gcgggcggtc
    16981 aaagccaatg gccgttacga ccgggtgttg atcggcaccg acactcccgg cggcaccggc
    17041 attgtgccgc gcgggattct gcgcgagatc gctttcatct cgtcggtggg cgaaatagcg
    17101 ccggaaaaaa ccatctgtat ggccaccggc aatgttggcc gcgctcacga tttgaacctg
    17161 ggcattttgg aggagggccg gcccgcggat gtagtcttga tgcacaagat cacgggttcg
    17221 gtctcgaagg atgcgctcga ctcgttcgcc aagggcgatt tgcctgggat ttcctacgtc
    17281 atcatcgacg gtgtcgttca agtagccggc cgcagccagc agacgccgcc gccggaagtg
    17341 ctggccaacc agtcttgttg ttagaggaga gtaatcgtga gcgcttatga atttggcgta
    17401 gggttatttc ccaccgaacc tttggagaaa atgattcaac tggccaaggt gagcgaggag
    17461 gtcggcttta gccatatctg ggtcggcgat tcgcatttaa tttggcgcga agcctacatc
    17521 aacatgacgg caatgatgct caataccacc aaagtgaagt tgggcaccgg cgtgactaat
    17581 ccgttgacgc gccatccgtc ggtgtcggcc agcggttatg cgaccctgga agaatattcg
    17641 ccggggcgaa tgattgtcgg catcgggctg ggcgatagct cggtggaaac catgggcatg
    17701 aagccgtcaa cgctggcgaa cttcgaaaaa tctctcgagc aaatgcgtca gatcctcgac
    17761 ggcaaagagg cgcaattgcc gaccggtaag atccacttgc tgcatccgac caagactcgc
    17821 gtgcccatct acatcgccgc cagcgggccg aagatgcttg agctctccgg tcgcatcgcc
    17881 gacggcatca ttgtgctggt cggtgtcgcc gacgattaca tcgcgcatgc gaaagagaaa
    17941 attgccgccg gcgccaaagc ggcgggacgg aagctggcgg atcttaattt agttctctgg
    18001 gtgccttgcg ccgtgtccga aacggcgaac gccaaagacg cggtcaaggc ccatgtcgcc
    18061 cgggtggtcg cccatccgct gccttatgtg ctcgatccca acgagcagaa agtattacaa
    18121 gagattcgca aaacctacga ctattaccat cacatggatc agcaggcgaa ccacgccgac
    18181 gtgattcccg actggctggt cgaaaaattc gccatcgccg gcaccgtcgc tcaatgcaaa
    18241 acgcaaatcg agcgcatcaa aaaaaccggc gtccagcaaa ttgccatcat tccctacagc
    18301 cctcccggcg gcagccgtga agagacgatt cgcggcttcg ccaaggcgat gaactaagcc
    18361 ctgccaatca agcgcttgca gcggtgagtt atcaacataa ctcgatataa ctcgatcgag
    18421 aattacgctg tgaattagct gacaagattg tcgttcaaag cgcattttcg tacggcgcgg
    18481 gtgcggtgaa gccttgtaat gcgttgaata cctgcgtttt acgctggcac gtgtgttgct
    18541 gcctcagaat atccgtagtg acgatcaagt atccaaacct atccgcggtt ggggataaaa
    18601 tatggcccaa cggcgtccag ttacaaaaag ccgagagg
//
LOCUS       DASQKS010000491         9284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1116115, whole genome shotgun sequence.
ACCESSION   DASQKS010000491 DASQKS010000000
VERSION     DASQKS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116115"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VE170_12700"
     CDS             complement(<1..258)
                     /locus_tag="VE170_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYJ16346.1"
                     /translation="MQIIEQIAGMRAWSAGERHLARRIVFVPTMGSLHEGHLCLVRDA
                     RTRGDRVVVSIFVNPMQFGPSEDFAKYPRDLQRDRAMLESLE"
     gene            complement(356..1159)
                     /gene="panB"
                     /locus_tag="VE170_12705"
     CDS             complement(356..1159)
                     /gene="panB"
                     /locus_tag="VE170_12705"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYJ16347.1"
                     /translation="MGDKITVPHIVKMKQRGEKITCLTAYDYSFARILDEAGVEMLLV
                     GDSLGCVVQGHANTLAVTMDEMIYHTRLVVRGRKRALVIGDMPFMSYQVGKDDALRNA
                     GRFFQEAGAEAIKLEGGVAVQESIRAIVDAGMPVMGHIGLTPQSVHQFGGYKIQGRDK
                     ERREAVLRDAVAVAEAGAFAVVLEGIPQDLASEISQRLAIPTIGIGAGLQCDGQVLVI
                     HDMLGLFDDFVPKFVKRYADVKQTMLGAVKEFVSEVKERKFPAEEHSFK"
     gene            complement(1276..1917)
                     /locus_tag="VE170_12710"
     CDS             complement(1276..1917)
                     /locus_tag="VE170_12710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HYJ16348.1"
                     /translation="MGKAKYIVVEGPIGVGKTSLARILADEFQSRRLFERVEDNPFLS
                     KFYQSRESYAFQNQTFFLLSRYRQQTELGQQDLFTEGTVADYLFAKDQIFAALTLSTE
                     ELSLYQQIYALLNTRVPKPDLVVYLQARPEVLYKRVKKRDKKYEKSVTFEYLTEVAQA
                     YNQFFFHYDETPLLVVNTSEIDFVSSSKDLADLIKEINGMGSGTQHYIPLGSR"
     gene            1978..3048
                     /gene="nagZ"
                     /locus_tag="VE170_12715"
     CDS             1978..3048
                     /gene="nagZ"
                     /locus_tag="VE170_12715"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HYJ16349.1"
                     /translation="MNPLRQKIGQLFLVGCQSEALTSDERLLIEEYQFGGLILFKNNC
                     AKPEQLVSLCQSIWQSFNAIPPFIAIDQEGGRVHRLPEPFSHFPAAARIGARNDPDLA
                     YRLGRATAAELMLAGINLNFAPVLDVNSNRQNPIIGDRAFAGGPRGVIEIASAWTKGL
                     RDGGIIPCGKHFPGHGDTDKDSHFELPLMRKSLEEIQATELPPFAEACRNQIEALMTA
                     HVTYPALDATLPATLSEVALTGLLRHQLGFDGVIFSDDLAMRAITDHYSVEEASDLAV
                     RAGVDVLLFCHEIDQAVAAFEFLCDAAGRNATMRARIDESYRRITDLKQRYLTGFTGA
                     AANEIVTRLERLNHRQLLTDFR"
     gene            complement(3169..4512)
                     /locus_tag="VE170_12720"
     CDS             complement(3169..4512)
                     /locus_tag="VE170_12720"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HYJ16350.1"
                     /translation="MAVIEKPEELEAYFQTAGKPGDRWRVGTEYEKVGVDRLTGQAIP
                     YFGPRGVDRILREMIERYGWEPDEQDGNIIALSRERTQITLEPGGQIELSGEPCDSIH
                     CTYAEFTQHIRELLEICEPLGITFLGLGMQPVSRLDEIQWVPKQRYRIMGPYMPTVGT
                     LGQRMMKQTATVQANIDFLDETDAMLKFRTGMGLAPVLIAMFANSPICDGRLNGYRSY
                     REHIWTDTDKSRQGLLKFAFAPDASFAHYVEYALDVPMYFIIRHHDYINMTGVTFRQY
                     LKYGHNGERATVEDWADHLTTLFPETRIKRYIEVRSVDSQAPELMPALSALIKGAFYD
                     NDCLLAVWDLVKGWTWDERNEVYLDSHANALAARFRRHTLLDLAREFLEIATEGLRRQ
                     HVVNDLGNDETIYLEPLKTLLSQGKCPADIIIEKWQGDLNRDVRKLIDFTAYKLP"
     gene            complement(4499..5530)
                     /gene="mtnA"
                     /locus_tag="VE170_12725"
     CDS             complement(4499..5530)
                     /gene="mtnA"
                     /locus_tag="VE170_12725"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HYJ16351.1"
                     /translation="MAVKTIEWKNDRVIMLDQRLLPHREIYRSYRNYNQVAEAIRSMV
                     IRGAPAIGVAAAMGVALGALYAPDRTFDREFERIVAVLGKTRPTAVNLFWALERMRAV
                     YSSNRTKGVAAVRRGLKEAALEIFKQDIAANKLIGRYGVPLLRDAKQIMTHCNAGALA
                     TAGYGTALGVLRALKQAGHRFEVYVNETRPFLQGARLTAWEFKQEKIPATLVTDNMVG
                     YLMQQGKVDAVVVGCDRVAANGDVANKIGTYTVAVLAKRHGVPFYVAGPTSSIDIDCP
                     TGKDIPIEQRDGKEVSHIFGKAIAPKGMKVFNPAFDVTDHELITAIITEHGVINPPYR
                     QNIRSHGSH"
     gene            5596..5838
                     /locus_tag="VE170_12730"
     CDS             5596..5838
                     /locus_tag="VE170_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16352.1"
                     /translation="MDFYLLAGYFSGLLFIKPDMEPATLWPTLALVHSLDAILCGVVA
                     DHSGRNKLLWTLSGLICGIWALGALFLLPARTSSGN"
     gene            complement(5943..6335)
                     /gene="acpS"
                     /locus_tag="VE170_12735"
     CDS             complement(5943..6335)
                     /gene="acpS"
                     /locus_tag="VE170_12735"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HYJ16353.1"
                     /translation="MIISTGIDLAEVDRIKAAIEHPRIGRRFRDRVFTKGEITYCEKK
                     QRGKYESYAGRFAAKEAVMKALGRGWGAKVRWLDIEVARARSGKPEIVLHDKTAQLAA
                     ELGICRWSLSITHTKQHGLANVIAEDDG"
     gene            complement(6332..6670)
                     /locus_tag="VE170_12740"
     CDS             complement(6332..6670)
                     /locus_tag="VE170_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ16354.1"
                     /translation="METSAVKCPAEFTLAMISGRWKIPIVFHLLAGRKRFSDLARALT
                     GVSQKMLTQQLREMERNGLVMRQVYAQVPPKVEYSLTALGASLRPVVEAMCHWGETHG
                     VQPEVWEAIL"
     gene            6778..7821
                     /locus_tag="VE170_12745"
     CDS             6778..7821
                     /locus_tag="VE170_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ16355.1"
                     /translation="MLQPALKLVENQFNGSLDAGARDLASRDGELLDAYSQAVVAAAE
                     KISPAVTFIEVTKEHRSSRHRPSGESGAIRGSGSGFIFTPDGLILTNSHVVHGAVKLD
                     VALNDGRHLSATMVGDDPSSDLAVIRIAAQNLSVVPLGDSEKTRVGQMAIAIGNPYGF
                     QCSVTAGVVSALGRSLRSVSGRLIDNVIQTDAALNPGNSGGPLVNSRGEVIGVNTAVI
                     LPAQGICFAVAINTAKFVVGQLIQNGRIRRAYLGVGGQNVDIPRAVARAQRLLHPTGV
                     LLISVEPSGPAERGELRVGDIIVALDNHPVRGIDDLHRLLTDARIGLPCNVELLRQSR
                     KINLNVVTHEAPS"
     gene            8112..>9284
                     /locus_tag="VE170_12750"
     CDS             8112..>9284
                     /locus_tag="VE170_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ16356.1"
                     /translation="MAKRSFLVFMLALLAALPATSWAQKGKIKIAVQAPLSGEQAALG
                     EHIKLGAQLAVEESVKAFKALGYDLVLVPQDDQAKPEVGVANARNMVADPEVLVIVGH
                     FNSGVALPASEVYKDAMLAMISPANTATEITDRGYPNVNRVCGRDDVQGPVGARFAAQ
                     ELKAKSVYIIHDKTLYGQGVADNFRNEAKKLGINVLGFEGTEERANFSPMINPLKAKN
                     PALVYFGGIYHQGGLLLKQLREKGVNAKFMGPDGLDSAEMAKIAGSSVVGSYYTSVAG
                     PPDAYPETAAFAKKYKQRFSKDVEAFGLYGYDATMVGIKAMEQWLKANPNKKPSRTEI
                     STAVRNIKGFKGVTGPIEFDSKGDPVKAKYFVLQFEKQSYPGKVVKVIEQQAPAAPA"
BASE COUNT         2000 a         2758 c         2526 g         2000 t
ORIGIN      
        1 ttcgaggctt tcgagcatcg cccggtcgcg ctggagatca cgcggatact tggcgaaatc
       61 ctcgctgggg ccgaactgca tcggattgac gaagatcgaa accacgacac gatcgcccct
      121 ggttctggca tcgcgcacca ggcagagatg gccctcgtgg agcgaaccca tggtcgggac
      181 aaaaacgatg cgccgcgcca agtgccgctc gcccgcactc cacgctcgca taccggcaat
      241 ctgttcgatg atctgcatgg aatatagaag attaagttgg agtaatcgaa tattgttgcg
      301 atgaccgatt cagaattcgg attgcccagt actccactac tccatcaatc cagatttatt
      361 taaacgaatg ctcctccgcc ggaaacttgc gttctttaac ttcactgaca aattctttca
      421 ccgcaccgag catagtttgc ttcacgtcgg catagcgttt gacgaacttc ggcacgaagt
      481 cgtcaaacag gccgagcata tcgtggatca ccagcacctg cccgtcgcat tgcaggccgg
      541 cgccgatgcc gatggtcgga atggccaagc gctgggaaat ctcgctagct aagtcttgag
      601 ggatgccttc taagaccacc gcaaacgctc cggcctccgc caccgcgacg gcgtcacgca
      661 gcacggcttc gcgccgttct ttatcgcgtc cctggatctt ataaccgccg aactgatgca
      721 ccgactgcgg ggtcaggccg atatggccca tgaccggcat gccggcatcg acgatggcgc
      781 gaatcgattc ttggaccgca acgccgcctt cgagcttgat cgcctcggcg ccggcttcct
      841 ggaaaaagcg cccggcgttg cgcaacgcgt cgtccttgcc gacctgatac gacatgaacg
      901 gcatatcgcc gatgactaga gcgcgcttac gcccgcgcac caccaggcgg gtgtgataga
      961 tcatctcgtc catggtcacg gcgagagtat tggcatggcc ctggacgaca cagcccaggg
     1021 agtcgccgac caacagcatc tcgacgccag cttcgtcgag aatccgcgcg aaggaatagt
     1081 cgtaggcggt gaggcaggtg attttctcgc cgcgctgctt cattttcact atatgcggta
     1141 cggtgatctt gtcgcccatg atctaccctc caataaaaca aaagccctcc agaccgagcc
     1201 ggaaggccaa taaccaaccg gagagccgct taactctccg taaccagcgc cttttggcat
     1261 ccgtctcggt ccagactacc tggatccaag cggtatataa tgttgcgtcc ccgaacccat
     1321 gccgttgatt tccttgatca agtcagccag atccttgctg cttgaaacga aatcgatctc
     1381 cgaagtattc accaccaaca gcggcgtctc gtcgtagtgg aaaaaaaact gattgtacgc
     1441 ctgggctact tcggtcaggt attcaaaagt aacgcttttc tcgtattttt tatccctctt
     1501 tttgacccgc ttgtaaagca cctctggacg cgcctggagg taaacgacta gatcgggctt
     1561 gggcacacgg gtgttcaata gcgcatagat ctgctgataa agacttaact cctcagtgct
     1621 caaggtcaaa gcggcgaaga tctggtcctt ggcaaataaa tagtccgcaa ccgtgccctc
     1681 ggtgaataga tcctgctgcc cgagctcggt ctgctggcgg taacggctca acagaaagaa
     1741 cgtctggttt tgaaaggcgt agctctcccg cgactggtag aacttggaca aaaacggatt
     1801 gtcttccacc cgctcgaaga gccggcgcga ctgaaactca tcggcgagaa tcctcgccag
     1861 actggtctta cccacaccga tgggcccttc gaccactata tacttcgcct tgcccattgg
     1921 catgagcgaa tttacacgtt ggccgaaagc ttgtctaggt tataatgacg caacgacatg
     1981 aacccactaa ggcaaaaaat cggtcagctg tttctcgtcg gctgtcaaag tgaggcgctc
     2041 acgagcgacg aacgtttgct gatcgaagaa tatcagttcg gcggcctaat cctgtttaag
     2101 aataattgcg ccaagccgga gcagctagtc tctttatgcc aatcaatctg gcaatccttc
     2161 aatgccatac cgcccttcat tgccatcgac caggaaggcg gccgggtcca tcggctgccg
     2221 gagccgttca gccatttccc agccgccgcg cgcatcggcg cgcgcaacga tcccgatctc
     2281 gcctaccggt tgggtcgtgc gaccgcggcg gaactgatgc tagcgggcat aaatttaaac
     2341 ttcgccccgg tgctcgatgt caattcgaac cggcaaaatc cgatcatcgg cgatcgcgct
     2401 ttcgccggcg ggccccgggg cgtgatcgaa atcgcctcgg cttggacgaa ggggctgcgc
     2461 gatggcggca ttattccctg tggcaaacat tttcccggcc atggcgacac cgacaaagac
     2521 tcgcattttg agttaccgct gatgcgcaag tcgctggagg agattcaggc caccgaactg
     2581 ccgcctttcg cagaagcttg ccgaaatcaa attgaagcgc tgatgaccgc ccacgtcacc
     2641 tatccagctc tcgatgcgac actgccggca actttatcgg aagtggctct cacaggtctc
     2701 ctgcgccacc aattgggctt tgacggggtt attttcagcg acgatttagc catgcgggcg
     2761 atcaccgacc attacagcgt cgaagaggcc agcgacctcg ccgtccgcgc cggcgtcgat
     2821 gtcctgctgt tctgccatga gatcgatcaa gccgtcgctg cctttgagtt tctctgcgat
     2881 gcagcgggcc ggaatgcgac catgcgagcg cgcattgacg aaagctatcg gcggatcacc
     2941 gacctcaaac agcgatattt gacaggcttc accggcgccg ccgcaaacga aatcgtcacg
     3001 cggctggagc ggcttaacca ccgccagttg ctcactgact tcaggtgatt cgactgcgga
     3061 ttctaaccgc aacacgcacg atccgactta ccgtggaaag gtatgcaagg cgctgttgtt
     3121 ctgtgttaag gttatgcccg agtgacggaa agcaacgttg cacgaaattt acggcagttt
     3181 gtaagcagtg aagtcaatta gttttctgac atcgcggttc agatcaccct gccatttttc
     3241 tatgattata tcggcaggac atttgccttg ggacaggaga gttttgagcg gttccagata
     3301 aatcgtttca tcgttaccta aatcgttgac cacatgctgc cggcgtaatc cttccgtggc
     3361 aatttccaga aactctctgg cgagatcgag cagcgtgtgg cgccggaagc gcgccgccag
     3421 cgcattggca tgagagtcga gatagacttc gttgcgctcg tcccaagtcc agcctttgac
     3481 caaatcccat accgccaaca agcagtcgtt gtcgtaaaat gcccctttga tcagcgccga
     3541 cagcgccggc atcagctccg gcgcttggct atcgacggaa cgcacttcga tgtagcgctt
     3601 gatgcgggtc tcgggaaaca gcgtggtcag atgatcggcc caatcttcga cggtggcgcg
     3661 ctcgccattg tggccatact tcaaatactg gcgaaaggtc acgccggtca tgttgatata
     3721 gtcgtggtgg cgaatgatga aatacatcgg cacgtctagg gcatattcga catagtgggc
     3781 gaaggaagcg tctggcgcga aagcaaattt caacaggcct tgccggcttt tatccgtgtc
     3841 ggtccagata tgctcgcggt agctgcggta gccgttcaac cggccgtcgc agatcggcga
     3901 gttggcaaac atggcgatga gcaccggtgc cagccccatg ccggtgcgaa atttcaacat
     3961 cgcgtcggtc tcgtcgagga agtcgatgtt ggcctgcacc gtggcggtct gtttcatcat
     4021 gcgctggcca agcgtgccaa cggtcggcat atacggtccc atgatgcgat agcgctgctt
     4081 cggcacccat tggatctcat ccaaacggct gaccggctgc atgcccagcc cgagaaaagt
     4141 aataccgagc ggttcgcaga tctctaacag ctcacggata tgctgggtga attcggcgta
     4201 ggtacagtga atgctatcac acggttcgcc gctgagttcg atctggccgc ccggctccag
     4261 agtgatttgc gttcgctcgc ggctgagcgc gatgatgttg ccgtcttgct catccggttc
     4321 ccagccgtag cgctcgatca tttccctcag gatgcgatcg acgccgcgcg gaccgaagta
     4381 gggaatggcc tgcccggtga gccggtcgac accgactttt tcgtactcgg tgccgacgcg
     4441 ccagcgatcg ccgggcttgc cggcggtctg aaagtaagct tcgagttctt cgggtttttc
     4501 aatgactgcc atgactgcga atgttctgtc ggtagggagg atttatcact ccgtgctcgg
     4561 taatgatagc cgtaatgagc tcatgatcgg tcacgtcgaa tgccggattg aataccttca
     4621 tgcccttggg cgcaatcgct ttgccgaaaa tatgcgacac ctctttgcca tcgcgctgct
     4681 cgatgggaat atcttttccg gtcggacaat cgatatcgat cgacgaggtc ggcccggcga
     4741 cgtaaaaagg aacaccgtgg cgcttggcta acacggcgac cgtgtaggtg ccgatcttat
     4801 tggcgacatc gccgttggcc gccacccgat cgcagccgac caccaccgcg tctaccttcc
     4861 cctgctgcat caaataaccg accatgttat cggtcaccag cgtggctgga attttctcct
     4921 gcttgaattc ccaagcggtc aacctagcac cctggagaaa cggccgggtc tcgttaacgt
     4981 aaacttcaaa tcgatgtccc gcctgcttca gcgcgcgcag gacgccgagg gccgtgccat
     5041 agccggcggt ggcgagcgcg cccgcattac agtgggtcat gatctgtttg gcatcgcgca
     5101 agagcggaac gccgtatctg ccgatcaatt tgttggcggc gatgtcttgc ttaaaaatct
     5161 ccaatgccgc ttctttgagc ccacggcgga ccgccgctac gcccttggtt cgattggaag
     5221 aatagactgc gcgcatgcgc tcgagcgccc agaataaatt taccgccgtc ggccgggtct
     5281 tgccgagtac ggcaacgatg cgttcgaact cgcgatcgaa agttcgatcc ggcgcgtata
     5341 gcgcgccgag cgccacgccc atcgccgcgg cgacgccgat agcgggcgcg ccgcgaatga
     5401 ccattgagcg gatcgcctcg gcaacctggt tgtaattcct ataactccgg taaatttccc
     5461 tgtgcggcaa cagccgttga tcgagcatta tcactcgatc gtttttccat tcgatagttt
     5521 ttaccgccac gcctagttcc aattcatggg agaccgagta tattaacgga ccaaaggaag
     5581 cgtcaagagc agctcgtgga tttctacctt ctcgccggat acttcagcgg cctcctattc
     5641 ataaaaccgg atatggaacc cgcgacgtta tggcccactt tggcgctggt ccatagtctc
     5701 gatgcgattc tctgcggcgt cgtcgccgat cacagcggaa gaaataaact cctctggacc
     5761 ctcagcggac tgatttgcgg tatctgggcc cttggcgcgc tgttcttact gccggcgaga
     5821 acatccagcg gtaactaaat tttcccaacg gcaaaagccc gccttgtcct gatcccgaat
     5881 ttcctctgaa tattattttg cgctctatgc ggctatcttc gtctttggcg ggcttgctgc
     5941 tctcacccat cgtcctcggc gatcacattg gccaggccat gttgcttggt atgggtaatc
     6001 gacagagacc aacggcaaat gcctagctcg gcggcgagct gcgccgtctt gtcgtgcaaa
     6061 acgatttccg gtttgccgct gcgcgcccgc gccacttcga tatcgagcca acgcacttta
     6121 gcgccccagc cgcgcccgag cgctttcatc accgcttctt tagctgcgaa tcgtccggcg
     6181 taactctcat acttaccgcg ctgttttttt tcgcagtatg tgatttcacc tttggtaaat
     6241 acccgatcgc gaaagcgccg gccgatgcgc gggtgctcga tggcggcctt gatgcgatcc
     6301 acttccgcaa gatcgattcc cgtgctgatg atcataggat ggcttcccaa acctcaggct
     6361 gcaccccatg tgtttcaccc caatgacaca tcgcttcgac caccggccgc aggctcgccc
     6421 ccaaggcggt gagggaatac tccactttag gtggcacttg ggcatagact tgtcgcatca
     6481 ccaaaccgtt gcgttccatt tcgcgcagct gctgagtgag cattttttgc gatacgccag
     6541 tcaaggcgcg cgctagatcc gaaaaccgtt tgcgcccggc caacaaatgg aaaacgatgg
     6601 gaatcttcca tcgtccgctt atcattgcca gggtaaactc cgccggacac tttaccgcgc
     6661 tagtttccat cgccgtctcc taggttactc tttggatact atagcaccgg cgggtgcgta
     6721 cttaacaagt attcccttcg tgattagctt cgccccaagc tgcgaaagga accgctcatg
     6781 ttacaacccg cgctcaagtt agtcgaaaat caattcaacg gtagccttga cgccggtgcg
     6841 cgcgacttgg ccagccgcga cggcgaactg ctcgacgcct attcccaggc ggtcgtcgca
     6901 gccgccgaaa aaatcagccc ggcggttacg ttcatagaag tcaccaagga gcaccgttca
     6961 tcgagacacc ggccaagcgg agagtcaggc gcaatccgcg gcagcggctc gggttttatc
     7021 tttacgccgg acgggctgat cttgacgaat agccatgtcg tccacggtgc cgtcaaactc
     7081 gatgttgcgt tgaacgacgg gcggcatctt agcgccacca tggtcggcga cgatccgagc
     7141 agcgaccttg ccgtcatccg catagccgcg caaaatctga gcgtcgtgcc actaggcgat
     7201 tcggaaaaaa cccgggtcgg ccaaatggcg atcgctatcg gtaaccctta cggctttcag
     7261 tgtagtgtca ccgccggtgt cgtcagcgcc ctcggtcgtt cgctacgctc ggtgtcgggg
     7321 cggttgatcg acaatgtcat ccaaaccgac gcagcgctca atcccggcaa ctctggcgga
     7381 cccctggtta attcacgagg tgaagtcatc ggcgtaaaca ccgccgtcat tctgccggcg
     7441 cagggaatct gtttcgcggt agcgatcaac actgcgaagt tcgtcgtcgg ccagttgatt
     7501 caaaatggcc ggattcgccg cgcttatctc ggcgtgggcg gccagaacgt ggatatcccg
     7561 cgtgccgtcg cccgggcgca gcgactacta catccaacag gtgtgctttt gatctcggtg
     7621 gagccgagcg gccccgcaga gcgtggcgaa ctccgagtcg gcgacattat cgtcgccctc
     7681 gacaatcacc cggtgcgcgg catcgacgat ctgcatcgtt tactcaccga cgcgcgcatt
     7741 ggcttgccct gcaatgtcga gctgctacgg caatcgcgaa aaataaatct aaatgtggtt
     7801 acccacgagg cgccgagctg attgccgatc ttttactcga aacccgctga cgagcggtca
     7861 gggtgccgct cagaggcggg ccacacaccg acagttagta ttcagaaccg gatcgcatca
     7921 ggcggcaatc ccaatgccgc gacggatcgc ctcgtcttgt cccacaaggc gatgtacggg
     7981 ttgccactcc tcgacccccg gagctttgac gtcgagccga tccagttcgg catcagccaa
     8041 gcagcaattt agtggcgccc gcagccacta tttgttatcg tgcgaccatt ccaactgaag
     8101 gagactctgc cgtggcgaaa agatcgttct tagttttcat gctcgcactg cttgccgcgt
     8161 tgccggcaac ttcctgggca caaaaaggta aaatcaagat cgccgtgcag gcgccgcttt
     8221 cgggcgagca agccgcgctc ggcgaacata tcaaactcgg cgcgcagctc gccgttgagg
     8281 aatcggtcaa ggcgttcaaa gcgctgggct acgatctcgt gctcgtgccc caggacgatc
     8341 aagcgaaacc ggaagtcggc gtcgccaacg cgcgcaacat ggtcgccgac cccgaagtct
     8401 tggtgatcgt cggacatttt aactcaggtg tcgcgctgcc ggcgtcggaa gtttacaagg
     8461 acgccatgct ggcgatgatc tcgccggcca ataccgccac cgagatcacc gatcgagggt
     8521 atccgaatgt aaatcgcgtc tgcggccgcg acgatgtcca aggcccagtc ggcgcgcgtt
     8581 tcgcggccca agagctgaaa gcgaaatcgg tctacatcat ccacgacaag acgctttacg
     8641 gccagggcgt ggcggacaat ttccgcaacg aggcgaaaaa gctcggcatc aatgtcctcg
     8701 gcttcgaggg caccgaagaa cgcgccaact tctcgccgat gatcaacccg ctcaaagcca
     8761 agaatcccgc gctggtctat ttcggcggca tctatcatca aggcggatta ttgctaaaac
     8821 aactgcgcga aaagggcgtc aacgccaagt tcatgggacc cgatggtttg gactcggccg
     8881 aaatggctaa gatcgccggc agctcggtgg tcggcagtta ctacaccagc gtcgccggcc
     8941 cgccggatgc atatcctgaa accgctgcct tcgcgaaaaa gtataaacag cgcttcagca
     9001 aggacgttga agcctttggc ttatacggtt acgacgccac gatggtggga atcaaagcca
     9061 tggagcaatg gctcaaagcc aatccgaata agaaacccag ccgcaccgag atttctaccg
     9121 ctgtgcgcaa tattaaaggt ttcaaaggcg tcaccgggcc gattgaattc gacagcaagg
     9181 gcgatccggt taaagccaag tatttcgtcc tgcaattcga aaagcagtcc tatcccggca
     9241 aagtggtaaa agtgatcgag cagcaagctc ccgcagcacc cgct
//
LOCUS       DASQKS010000492         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_246851, whole genome shotgun sequence.
ACCESSION   DASQKS010000492 DASQKS010000000
VERSION     DASQKS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246851"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VE170_12755"
     CDS             <1..813
                     /locus_tag="VE170_12755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16357.1"
                     /translation="PIRDTLSAGVQSLLGVDVLIGFGNPLAVLQPLASGGDITVIGSH
                     VSFDQYGMGVATSIDAVKDLKGKKVGVSALGARSDLIARVMLRRAGLDPAKDVEMVAA
                     GLAPQRALALSKNLVQGVPLREDIAAEAKKAGIKIIEVKSVPVVTDLLMTTRSFIKRD
                     EETIRRFMKGYAAAIHHFLSKRAESLAILKKYFPGTQGVSVDSMYDAYAAQLRPLPEL
                     NNESIQALVDVSAAADQRAGKLKLNDIVEQRFFDELKNSKFLKDLYVEKVSL"
     gene            1020..1766
                     /locus_tag="VE170_12760"
     CDS             1020..1766
                     /locus_tag="VE170_12760"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYJ16358.1"
                     /translation="MRLKDQVAIVTGAGRNIGEDVCKLFVAEGAKVAVVDLDPGRGSS
                     VANEINAKNPGKAISVVCDVANAASVNTMVETVVKQFGGVDILVNNAAWTDHKTLFDI
                     TEEEWDRTMNVCMRSVWYCTRAAAKVMIDQKRKGRIVNIASTSGHWGRKEATAYTTAK
                     AGVLNLTRSIAVQLAPEGIRVNSVSPNRIGSPVGQQDVPENRFVKNLAGRRGVPMDIA
                     KAVVFMVSDESEFIYAIDLPVDGGALAIRD"
     gene            2054..>2704
                     /locus_tag="VE170_12765"
     CDS             2054..>2704
                     /locus_tag="VE170_12765"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HYJ16359.1"
                     /translation="MSETPIQAAIQADATLKSTPETVLWGYIAANLPPALSVKSGQIV
                     EIEALSHQGLTTGKDPEKFFAGYGIPANEVLADAKNIYSEVKRPKGASVHILTGPIYV
                     DGAEPGDTLEIRVHDIKFRVPYGVNNTGPGKGVLPKLLKEATAKLIRIDLERRVALFS
                     DDIQIPLNPFMGIMAVSPPTSLGLVSSTPPGAWGGNIDLKYTGIGSSLFLPVFNKGG"
BASE COUNT          605 a          727 c          797 g          575 t
ORIGIN      
        1 ccgatccgcg acacgctgag cgccggtgtg cagtcgcttc taggcgtcga tgtgctgatc
       61 ggtttcggca atccgctcgc cgtactgcag ccgctggcta gcggcgggga tatcacggtg
      121 atcggttccc atgtcagctt cgatcagtac ggtatggggg tggcgacatc catcgatgca
      181 gtaaaagatt tgaaaggaaa gaaagtcggc gtttccgcgc tcggcgcacg ctcggatcta
      241 atcgcgcgcg tcatgttgcg gcgtgccggg ttggaccccg ctaaggatgt ggaaatggtc
      301 gcggccggtt tggcgccgca acgggcgctc gcgttgtcta agaatctcgt ccagggagtg
      361 ccgctcaggg aagatatcgc cgcagaagcg aaaaaagccg gcatcaaaat cattgaagtc
      421 aaaagcgtgc cggtggtcac tgatttgttg atgacgacgc gctcgttcat caagcgcgac
      481 gaagaaacca tccgtcgttt catgaagggc tatgccgcgg cgatccatca tttcctttcc
      541 aagcgcgccg agagccttgc gatcctgaaa aaatatttcc ccggcactca aggcgtctcc
      601 gtggacagca tgtatgacgc gtatgcggcg caactacggc cgctacccga gctcaacaac
      661 gaatcgattc aagccctggt cgatgtcagc gccgccgccg accagcgcgc cggcaaactc
      721 aaactcaacg atatcgtcga gcaacgcttt ttcgacgagc tcaaaaacag caagtttttg
      781 aaggatctct acgtcgaaaa ggtgagtctc tgacggtccg ctagagtcaa attcaaaaat
      841 aaatcaggga ttgaagatgg agatcgcgtc ggcggttgtc catttctcgc ggttcgtcgt
      901 tttcctcgat ggcctaagcc tacttcggca cacatgtctt tggctgttag aacccctcaa
      961 ctggaactta tccaggcctt ttgatatgca gcagagaaac aaattcaatc ggaggactca
     1021 tgagacttaa ggatcaggtg gcgatcgtca ccggcgcggg gcgtaatatt ggcgaagacg
     1081 tgtgcaaact gttcgtcgcg gaaggcgcca aggtcgccgt cgtcgattta gatccggggc
     1141 ggggcagtag tgtcgccaat gaaatcaatg cgaagaatcc tggcaaagct atttcggtgg
     1201 tttgcgatgt cgccaacgcg gcgtcggtta acaccatggt cgagaccgtg gtcaagcaat
     1261 tcggcggcgt cgatatcctg gtcaacaacg cagcttggac cgatcacaaa acgctgttcg
     1321 acatcaccga agaagagtgg gaccggacga tgaacgtgtg catgcgcagc gtatggtact
     1381 gcactcgcgc ggcggccaag gtgatgatcg accagaagcg caaaggcagg atcgtcaaca
     1441 tcgcctcgac ttcgggccac tggggccgca aagaagcgac ggcttatacc acggcaaagg
     1501 cgggcgtatt gaacctaacc cgttccatcg ccgtgcaact ggcgccggag ggcattcgcg
     1561 tcaattccgt ttcgccgaat cgcattggct cgccggtggg ccagcaagac gtgccggaaa
     1621 atcgcttcgt gaagaatttg gccggccggc gcggcgtgcc gatggacatc gccaaggctg
     1681 tggtgttcat ggtttccgat gagtcggagt ttatctatgc tatcgacttg ccggtcgacg
     1741 gcggcgcgtt ggcaatccgc gattagttgc tctttcgctc atcattaaag gcggcgaggc
     1801 gacatggctt cgccgttttt ttttgggtta cgccgatata gatgcattcg ccgcacaggc
     1861 tgagctcgcg aaggggcacc gaaccgaatt cggaaccggg agttaatcgc aaagggcgcg
     1921 agaaacgcaa aatggttcct agagaaattc cgatggagaa gcgtttttta aaaccacctc
     1981 ccctgctttt ccttctggtt gcgttgacag tcttttgtag gatcggataa cttcgccgcg
     2041 aagaggacca gccatgagtg aaacccctat tcaagcagct attcaggccg acgcgacctt
     2101 gaagtcgaca cccgagacgg ttttgtgggg ctatatcgcg gcgaatttgc cgccggcatt
     2161 gtcggtcaag tcgggacaga tcgtcgagat cgaggcgctg tcccatcagg gattgaccac
     2221 cggcaaggat cccgagaagt ttttcgccgg ctacgggatt cctgcaaatg aagttctcgc
     2281 cgatgccaag aacatttact ctgaagtgaa gcggcctaag ggcgcaagcg tgcatatact
     2341 caccgggccg atctacgtcg acggcgcgga accgggcgat actttagaga tccgcgtgca
     2401 cgatatcaag tttcgcgtgc cctatggcgt caacaatacc gggcctggca aaggcgtgct
     2461 gcccaagctg ctcaaagaag ccacggcgaa actgatccgt atcgatctcg aacggcgcgt
     2521 ggcgctattc tccgacgata ttcagatacc gctcaatccg ttcatgggca tcatggcggt
     2581 gtcgccgccg accagtttgg gactggtttc atccacgccg ccgggcgcct ggggcggcaa
     2641 catcgatttg aaatacaccg gcatcggctc gtcgttattt ttaccggtgt tcaacaaggg
     2701 cgga
//
LOCUS       DASQKS010000493         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3216983, whole genome shotgun sequence.
ACCESSION   DASQKS010000493 DASQKS010000000
VERSION     DASQKS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216983"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..3763
                     /locus_tag="VE170_12770"
     CDS             443..3763
                     /locus_tag="VE170_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HYJ16360.1"
                     /translation="MGQEEYQEISSFLRRFSRRFKLLRGVEGICLTAICAVLLFATGL
                     GIYQLRQLLPYAPLAYSILTGIVLIVAIGWTLFRFLGRLSQERAALYIEQQQPKLRNN
                     LINSLQLYPQVAEAKNTPGFSTTMVFALLRATLKQVAPLKIDELLDHSRLRSSARLLA
                     FLIVPVLAAVFLNPSGVGGTLNMLANPLDHLPPSQTTIEVEPKGLRVVRGSPVTIQAS
                     TAGALPQSLELITWQGTNAKGEIIGQEKFPMENLGGGKFSSKIARLDKTLRYRVMTGA
                     FTSATFTAEAADTPEIANVQVMLYPPAYTGLASISSPEGSIEGLKGSSLRLDAVTTKD
                     VVKAEIVMDDGKKIPLKVDGRKLQANLVLFQSQSYRIQVEDSHGFKNSPISYELRVKP
                     DGFPTVDLLRPTEDLEVNGDETLNLEYSARDDFGIGEINLIVKIGEREDKIRLARDDS
                     KRVILRDQYKMDLGRLGLRDQDEALFFLQVFDNDTISGPKLGTSKTIRLKLKNLKGEH
                     QQIAEMVKDLNARMMNLLADHLESPNPGDKPTAQAKELEQRFEQNLADAAKRTEEIMR
                     RTEKDRMTDFATYSDLQALKRNLDFTKDDLLRKQEQAANPDDKLKARDEISSELERMS
                     LLSEDIGKRMKAQELASTAQDLARNQERLMDSLDKLQSGDKNLDAIMKQIADLAKSLM
                     DLQQAMAQLAQQMPDDFMNAEQMQGLGMDEMFKALDEIRKKLQAGDIEGARQMARELF
                     NQMAQMVAAMQNMQRSQMASSMGRMQGEMQRQTNELQEIAREQQEILVKTENLNSAGL
                     TERDAALKSKLEQFLARSIAELGKLTELFPDREGPDDPNLLTNLDDATMNALLRNLIA
                     KLSQKDFPGFNEGDGYIRKELAKKRTPVQEPKAQRADQSISDIKNELDKLLNEPAQAL
                     SDADKRALRDLAQREDVLKERTETLHEKLESLFQLFPQLDPKIVQGIGDAGKAMGSAQ
                     GRLGQLDSRGAVPPERQALERLGEAQQQMQQSMQQMAQRGQMGNMPMTRLFRQGGPGR
                     FLPFGSLTPLPGMPQFPEFDFEQGFTGLDTEKFRLPGKEEYKAPRNFREEILDSLKQG
                     VPRQMKEQIERYFKNLSE"
     gene            3774..>4869
                     /locus_tag="VE170_12775"
     CDS             3774..>4869
                     /locus_tag="VE170_12775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ16361.1"
                     /translation="MYRIAPFLILFGLLAGPLFGAEVEREFKAGEEILEAWRFADAEA
                     IAAKALKDNPKSATALEFDGRIKFYQGRYQEALTVLDRALAIDSKDPRRQAMKLLTQL
                     TLDVHKSLKRHESAHFILFVDDKRDAILVPHALDALEKSYEAIGAELGYYPKEKVRVE
                     VAPDATSFNAISTLSLRDIEETGAVGICKFNKLMIISPRALSFGYRWVDSLSHEYLHY
                     VIVSLSNNQAPIWLHEGMARFYETRWRKPAPATEAHEDYLTPANQTLLVQALDKNQFV
                     GFKKMEPSLIHLDTPEQVQLAYAEAASAIDFINQSKGKAGIRELLATLKDKPTTEAVE
                     KVYGMGFDAFETKWKAFLKAKGLKPIEGTRV"
BASE COUNT         1172 a         1405 c         1363 g          929 t
ORIGIN      
        1 tttagcagtg cgacagaatg gttgggcact gaccaatcca gcgatctcgg ctgctcacct
       61 gcttgccgtt catgtaatta tcacgccgat gttgtcagtg agtaacgtct tttggccacg
      121 ggtggtgtat cgaatgtgct tcacgtagcc cggcatggag gacaatcggc aaaaggctag
      181 caaagaaatc ttgtgctagg aatcgatggc gaccggatga ttgatgcgca ttcttggtag
      241 gaaaacgaca cctttggtcc aggattgcgc ggctcaattt gttccgcagc cgcataaatt
      301 ttctcggtag cgagtaaaat tttgcgtgaa atatcgcgct cggacgattg tgagtgccgc
      361 agcaaaacct ctctgggcaa aatgtgttgc gggatggaca agccccccta aacgacgata
      421 agatgctttt cggtttcaga ttatgggaca ggaagaatat caagagatct cctcgttcct
      481 gaggcgcttc agccggcgct tcaagctgct ccgaggggtc gagggaattt gcctgacggc
      541 gatttgcgcc gtgctgttgt tcgccaccgg tctcggcatc tatcagctgc gccaactcct
      601 gccctatgcg ccgctcgctt actcgatatt aaccggcatc gtgcttatcg ttgcgatcgg
      661 ttggacgctg tttcgctttc tcggccgttt gtcgcaagaa cgggcggcgc tttatatcga
      721 acagcagcag cccaagctgc gcaacaatct catcaactcg ctgcaactct atccccaggt
      781 cgccgaggcg aagaatactc ccggtttttc gacgaccatg gtgtttgcgc tcctgcgcgc
      841 gaccctcaag caggtggcgc cgctgaaaat tgacgaactg ctcgaccaca gccggcttcg
      901 ctccagtgcg cggttgctcg cttttttaat cgtgccggtg ttggcggcgg tatttctcaa
      961 tccctctgga gtgggcggga cattgaacat gttggccaat ccgctcgacc acctgccgcc
     1021 gagtcagaca actatcgaag ttgaacctaa gggactgcgt gtcgtccgcg gcagcccggt
     1081 gacgatccaa gcgtcaacgg ccggcgcgct accccaatct ctcgaactga ttacctggca
     1141 gggaaccaat gccaagggcg aaattatcgg ccaagaaaaa ttccccatgg aaaatcttgg
     1201 cggcggcaaa ttctcgagca agatcgcccg cctcgacaaa accttgcgct accgggtgat
     1261 gaccggcgcc tttacctcgg caacgttcac cgccgaagcg gcggatacgc cggagatcgc
     1321 caatgtgcag gtcatgctct acccgcccgc ctacaccggt ttggcatcga tatcttcgcc
     1381 ggaaggaagc atcgaagggc tcaaaggttc ttctctgcgc cttgacgcgg tcaccaccaa
     1441 agatgtggtc aaggccgaaa tcgtcatgga cgacggcaag aagatcccgc tcaaggtcga
     1501 tggccggaag ctgcaagcga atctggtgct gtttcagtcc cagtcgtacc gcattcaggt
     1561 cgaggacagt catggtttta agaattcacc gatcagctac gagctgcgcg tcaagcccga
     1621 tggttttcct accgtcgact tgctcaggcc caccgaagat ctcgaagtaa acggcgacga
     1681 gacattaaat ctagaataca gcgcgcgcga cgatttcggc attggcgaaa ttaatctaat
     1741 cgtcaagatc ggcgaacgcg aagataagat tcggctcgcc agggacgatt cgaagcgggt
     1801 gattctgcgc gatcaataca agatggattt gggccgactc ggcttgcgcg accaagacga
     1861 agcgctgttt ttcctgcaag ttttcgacaa cgacaccatc tccgggccta aactgggcac
     1921 ctcgaagacc attcggttaa agctcaaaaa tttaaagggc gaacaccaac agatcgccga
     1981 gatggtcaag gacttgaacg cgcgcatgat gaatctcctg gccgatcatt tagaatcgcc
     2041 caatccaggt gacaagccga cggctcaggc gaaagaattg gaacaacgct tcgagcaaaa
     2101 cttggccgac gcggccaagc gcaccgaaga aatcatgcgg cgcacggaaa aagaccgcat
     2161 gaccgacttc gccacctatt ccgatctcca ggcgctcaag cgcaatctcg acttcaccaa
     2221 ggacgatctt ttgcgtaagc aagagcaagc cgccaatccc gatgacaaac tcaaggcgcg
     2281 cgatgagata tcgtccgagc tcgagcgcat gtcgctgttg tccgaggaca tcggcaagcg
     2341 catgaaagcc caagagctcg cttcgacggc gcaggatctg gcgcgcaacc aagagcggct
     2401 gatggactcc ctcgacaaat tgcaaagcgg cgacaagaat ctcgacgcga tcatgaagca
     2461 gatcgccgat ctggcgaaat cgctgatgga tttgcagcag gccatggcgc agttggcgca
     2521 gcaaatgccc gacgatttca tgaatgccga gcagatgcag ggactcggca tggacgaaat
     2581 gttcaaagcc cttgacgaga tccgcaaaaa gctccaggcc ggcgacatcg agggggcgcg
     2641 ccagatggcc cgtgagctgt tcaatcaaat ggcgcaaatg gtcgcagcga tgcagaacat
     2701 gcagcgctcg cagatggcct cgagcatggg gcgcatgcag ggcgaaatgc agcgccaaac
     2761 caacgagctt caagaaatcg ctcgggagca gcaagagatt ttggtcaaga ctgaaaatct
     2821 caatagcgcc ggcctcaccg aacgggacgc cgcgttaaaa tcgaagctgg aacagtttct
     2881 ggcgcgctcc atcgccgagt tgggcaaact caccgaactg ttccccgacc gcgaggggcc
     2941 cgacgatccc aatctgctca ccaacttgga cgacgccacc atgaacgcgc tgctgcgcaa
     3001 cctgatcgcc aaactaagcc agaaggattt ccccgggttc aacgaaggcg acggttatat
     3061 ccgcaaagaa ctggcgaaaa agcgcacccc ggtgcaggag cccaaggcac agcgggccga
     3121 ccaaagcatc agcgacatta agaacgagct ggacaaattg cttaacgaac cggcgcaagc
     3181 cttgagcgac gccgataaac gcgcgctgcg cgacttggcc caacgcgagg acgttttgaa
     3241 ggaacggacc gagacgctgc atgaaaagct ggaatcgttg tttcagctct tcccccaact
     3301 cgaccccaag atcgttcagg gcatcggaga tgcgggcaag gccatgggca gcgcccaggg
     3361 ccgtttaggc cagctcgatt cccgcggcgc ggtgccgccg gaacgccagg cattggaacg
     3421 gttgggcgaa gcgcagcagc agatgcaaca gtcgatgcag caaatggccc agcgcgggca
     3481 gatgggcaat atgccgatga cccggctgtt tcgccaggga ggaccgggtc gcttcttgcc
     3541 gttcggcagc ctgacgccgc taccgggcat gccgcaattc cccgaattcg actttgaaca
     3601 gggcttcacc gggctcgaca cggaaaagtt ccgactaccg ggcaaggaag aatacaaagc
     3661 accgcgcaac ttccgcgaag agattctcga ctctctcaag cagggcgtgc ctcggcagat
     3721 gaaagagcag atcgagcgct atttcaaaaa tctttccgag tgatcgccgc ttgatgtacc
     3781 gaatcgcgcc gtttctcatt ctcttcggtt tattggccgg gccgctcttc ggcgccgaag
     3841 tcgaacgtga attcaaagcc ggcgaggaga ttcttgaagc ctggcgcttc gccgacgctg
     3901 aggcgatcgc cgccaaagcg ctcaaggaca atcccaagtc ggccaccgcc ctcgagttcg
     3961 acggccggat taaattttac caaggccgtt accaagaagc gctgaccgtt ctcgaccgcg
     4021 ccctggcgat tgattcgaag gatccccggc gccaggcgat gaagctgctc acccagctga
     4081 ccctcgacgt ccataagtca ttgaagcgcc atgagagcgc ccatttcatt ctgttcgttg
     4141 acgacaagcg cgacgccata ctcgtgccgc acgccttgga tgccttggaa aaaagctatg
     4201 aagcgatcgg cgccgaactc ggctactatc ccaaggaaaa agtccgcgtc gaggtcgccc
     4261 cggacgcgac tagttttaac gccatctcga ccctgtcgct gcgcgacatc gaagagaccg
     4321 gcgccgtcgg catctgcaag tttaataaat tgatgatcat ctcgccgcgg gcgttgagct
     4381 tcggctaccg ctgggtcgac tcgctgagcc atgagtattt gcactacgtc atcgtctcct
     4441 tgtcgaacaa ccaggcaccg atctggctgc atgaaggcat ggcgcgcttt tacgaaaccc
     4501 gctggcgcaa accggcgccg gccacagagg cccatgaaga ctacctgacg ccggccaatc
     4561 aaaccttgct tgtgcaggcg ctggacaaaa atcagttcgt cggtttcaaa aaaatggaac
     4621 cgtcgctaat tcatttagac acgccggaac aggtgcagct ggcctacgcc gaagccgcct
     4681 cggcgatcga ctttatcaat cagagcaagg gcaaagccgg catacgcgaa ctgctcgcaa
     4741 ccctcaaaga caaaccgacc acggaagcgg tggaaaaagt ctacggcatg ggattcgacg
     4801 cctttgaaac caagtggaag gcgtttctca aggccaaggg gttgaagccc atcgagggca
     4861 cacgggtgc
//
LOCUS       DASQKS010000494         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1531648, whole genome shotgun sequence.
ACCESSION   DASQKS010000494 DASQKS010000000
VERSION     DASQKS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531648"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1110
                     /locus_tag="VE170_12780"
     CDS             106..1110
                     /locus_tag="VE170_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16362.1"
                     /translation="MRIARLYLLLLALVGSLLALESHAQVSKTTTSYVSDSAGVLPLW
                     LAKEAGIFARNGLDVQAVRVHAAVGVMALMSGELQFVLISGPVAVESALRGSNMVYVA
                     GGMSTLDFVLVTRPEIKTAEQLKGGTVGLSAIRGANPTATRFALQKLGLAPKDVSLQV
                     IGGTSERIASLRLGKIQGTLFSPPQSITAQREGFNALADVAELGLPFVHIGVATTRAV
                     IRDRPELVRRYVKSHIEAIALMKTDRDLAIKVLAKYLGQIKEREVLAKSYDTAAPDNR
                     ISRKQYPSVAGLQMALDLLADDNPKAKGAKPEDFIDNRFIKELDDSGYIDSLYKPKPR
                     "
     gene            1246..2163
                     /locus_tag="VE170_12785"
     CDS             1246..2163
                     /locus_tag="VE170_12785"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16363.1"
                     /translation="MLVVSIWPATIDARMVVGVSTVNVAFLPIYLTQDKGFFKDEGLD
                     VLVVMFNAGATNLQAMIGGDVQVMAGGLPESVLARAGGLDIKNFWAISNLMPFQIYGS
                     PSMKSLKDGKGKKFAISRFGSLSEFLTRSALRQAGVDPKDVTMLQIGATPARFTTLAS
                     GIVDATILWFPVTERAKSAGLNKLFDLKDLYPNWTNVGFIARESWLAKEREQATKFLR
                     AYQRGVKHTRENRDDGIRALRKYVGLNAAEAAAGYDEYRDSFPIDGRILDAGITATVE
                     QEIETGRLKGKISLGEMIDGSFINLLGKK"
     gene            2192..>2396
                     /locus_tag="VE170_12790"
     CDS             2192..>2396
                     /locus_tag="VE170_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16364.1"
                     /translation="MFIVSIRIFAAVAAFWLCAGGSAQAKPITLAYNTNSLNVMLPLL
                     VAQELGFFTAEGFEVTPVYVRSGP"
BASE COUNT          497 a          675 c          711 g          513 t
ORIGIN      
        1 gaggttcttc gccgtcaatg gttcgtcgcg agattaagtt gagcgccatt gacggcgcgg
       61 cgccgcttcg gttagtttcc tggccatggg ctttgagggc tgccaatgag aatcgctaga
      121 ctgtatttgc tgcttttggc actggttggg agtttgttgg cgcttgagag ccacgcccag
      181 gttagcaaaa ccaccacttc ctatgtttcc gattcggccg gcgtcctgcc gctctggctc
      241 gccaaggaag ccggtatctt cgcgcgcaac ggcttggacg tgcaggcggt gcgcgtgcac
      301 gccgcggtcg gagtcatggc gctgatgtcc ggcgaactgc aattcgtgct gatatcagga
      361 ccggtcgcgg tcgaaagcgc cctgcgcggc tccaatatgg tttatgtcgc cggcggcatg
      421 tcgacgctgg actttgtcct ggtgacccgg ccggagatca aaaccgccga acaactcaag
      481 ggcggcaccg tcggtttgtc ggcgatccgc ggcgccaatc cgaccgcgac tcgattcgcg
      541 ctgcaaaagc ttggcctggc cccgaaggat gtatcgctcc aggtgatcgg cggcacgagc
      601 gagcgaatcg ccagcctgcg cttgggaaaa atccaaggga ctttgttcag tccgccgcaa
      661 agcatcacgg cgcaacgcga gggtttcaat gctctggcgg atgtcgccga actcggcttg
      721 ccgttcgtgc atatcggcgt ggccaccacc cgcgccgtca tccgggacag accggagctg
      781 gtgcgccgct acgtcaaatc ccacatcgag gcgattgcgc tgatgaagac cgatcgcgac
      841 ttggcgatca aggtgctggc gaagtatctt gggcaaataa aagagcgcga agtgctggcg
      901 aaatcctatg acaccgccgc gccggataac cggatctcgc gcaaacaata tccgtccgtc
      961 gccggtctgc agatggccct cgatttattg gccgacgata atcccaaagc taagggtgct
     1021 aaaccggagg acttcatcga caaccgtttt atcaaagagc tcgacgacag cggctatatc
     1081 gacagtctat acaagcccaa gccgcgctga gcgaaaacaa tttcgcgctg gcgtgctgcg
     1141 gtatcaactt gatcgaaact tttgacctat ctaggctccg tggccagggg gaaacaaatg
     1201 gttgagcgcc gatttcggtt atgcccaaaa ctgctaacac ttctattgct cgtcgtcagc
     1261 atctggcctg ctaccatcga tgcccggatg gtcgttggcg tatcgacggt gaatgtcgcc
     1321 ttcttgccga tctatctgac gcaagacaaa ggcttcttca aggacgaggg cttggacgtt
     1381 ctcgtcgtca tgttcaacgc cggcgcgacc aatcttcagg cgatgatcgg cggcgacgtg
     1441 caggtcatgg ccggcggctt gccggagtcg gtgctggcca gggccggcgg tttggatatc
     1501 aagaattttt gggcgatctc caatcttatg ccgtttcaga tctatggcag cccgagcatg
     1561 aaatcgttga aggacggcaa agggaagaag tttgcgatca gccgcttcgg ctcgctgtcg
     1621 gaatttctca cccgttcggc gctgcgccag gccggagtcg atcccaaaga cgttaccatg
     1681 ttgcagatcg gcgccacgcc ggcgcgcttt accaccctgg cgagcggcat cgtcgatgcg
     1741 acgattctct ggtttcccgt caccgagcgc gccaaatcgg ccggcttgaa taagctcttt
     1801 gatctgaaag atctctatcc caactggacc aacgtcggtt ttatcgcccg cgaaagctgg
     1861 ctcgccaaag aaagagagca agcgacgaaa tttctacgcg cttatcagcg tggcgtgaag
     1921 cacacccgcg agaatcgcga cgatggcatt cgcgccctga gaaaatatgt tgggctaaac
     1981 gccgccgaag ccgccgccgg atacgacgag taccgcgatt cctttccaat cgacgggcgc
     2041 atcctcgacg ccggtattac cgccacggtc gagcaggaga tcgagaccgg caggctcaag
     2101 ggaaaaattt cactgggcga gatgatcgac ggcagcttca tcaatttgct cggaaaaaag
     2161 tagggttggt ttatttaagg gaagccagaa catgttcatc gtttcgataa gaatttttgc
     2221 cgccgtggcg gcgttttggc tctgtgccgg cgggagcgct caggccaagc cgatcacgct
     2281 ggcgtacaat accaattcgc tcaacgtgat gttgccgctc ctagtggctc aggagttagg
     2341 attttttacc gctgaaggtt tcgaagtcac cccggtttac gtgcgcagcg gaccga
//
LOCUS       DASQKS010000495         3720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3649145, whole genome shotgun sequence.
ACCESSION   DASQKS010000495 DASQKS010000000
VERSION     DASQKS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3649145"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2580)
                     /locus_tag="VE170_12795"
     CDS             complement(127..2580)
                     /locus_tag="VE170_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HYJ16365.1"
                     /translation="MADKIIRLDSRRDVEAIAWAKELRETEGWFHSSRFRHITRLHTA
                     AEVVSLRGNLQADHTIAKQSALKLFDYLQRLFRDKKQEITYGPYSPTGAVRAVMEGIK
                     VLYLGGWATSAKGSEAEDPGADLANYALDRVPKEGGAWVRALLHQDEVQRSRRIRMSS
                     LQRKKTRAIEFSPPLIIPDGDTGHGGEHHTRNLVKTFVENNIGAIHIEDQRAGSKVCG
                     HQGQKVLVSTSEMISRLNAARLQYDIMHVPGVIVARTDSNDATAIDSVDDDRDHPFVY
                     GATNPDIVPFKNVSLAVIRKFYQKGFKEINGHLLYKISEQAYAGAEKWLRQERLLDRI
                     EAGIDRIDSEIVALKKLRQRVRRPGKGQRDFREQLAAQEIAVKKVIEETVDEVVAGIR
                     QEWADKARLKTYAEAVAEVLASGAGKLAMSAERWKKFAAGLSHAEARAGAAAMGVDIF
                     WDWDLPRTPEGFYVITGGRAMATARGLATAPFADLIWRETAKPDLADDKAWADEIHAV
                     HPEKMLAYNLSPSWNWDVWGFTDDQIRNFASELGKMGYVFNFITYGGHQTEALMNGRL
                     ARALKEEGVLGFVRLIQRALRLAHDPAQFPQSFVGGDWADRFRRAARGASLTSSSMGG
                     KSTETQHRKAVEVPTSVLVKWLAMWAEHWHRQGLYQGGALSVELKERWAGSEDMMLNL
                     FDEAHDKIAEIIFRADKDRAGRKFLAVKDQNTVKKFRSRRLMTLMHFFLLHRYKTELV
                     HYVTPTNDNRLSVQRMIQNGVFRAARTDDPHIIAIEVDNARGQKIFANEDSIRKFIAR
                     QFKPVERPSDSRRTAGRGG"
     gene            complement(2673..3104)
                     /locus_tag="VE170_12800"
     CDS             complement(2673..3104)
                     /locus_tag="VE170_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYJ16366.1"
                     /translation="MNEKFRNPFPTVDIIIEIAGGIVLIERKNPPPGWAMPGGFVDYG
                     ESVERAAVREAREETCLEVNLTEQFYTYSDPRRDPRHHTLAVVFIATAQGTPRGADDA
                     RVAKIFFENNLPAPLAFDHAVILRDYFHFKKTGQRPKPALD"
     gene            3166..3242
                     /locus_tag="VE170_12805"
     tRNA            3166..3242
                     /locus_tag="VE170_12805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3200..3202,aa:Met,seq:cat)
     gene            3405..>3720
                     /locus_tag="VE170_12810"
     CDS             3405..>3720
                     /locus_tag="VE170_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16367.1"
                     /translation="MKIMIPYAFLILVFLLTPAAASNHYTEKQLNALEARVGKMFWIS
                     PVEEKLPSFVTSPASSAAAFQPSANESFEITELVGRKAKNPYYKVKFESGKEGYIHAQ
                     AFH"
BASE COUNT          737 a         1110 c         1053 g          820 t
ORIGIN      
        1 ccgtgcccgg tgcgatctcg tcgcgcacca gacgataaag ctcttcgtcg attcgtaacc
       61 cggatgtttc gatatagttt gccatgggcc gatgcttcct tccccaatcg attaaaggca
      121 ccgcggtcac ccaccgcggc ccgctgtgcg ccggctgtcg ctcgggcgct ccaccggttt
      181 gaactgccgc gcgataaact tccggatcga gtcttcgttg gcgaagatct tctggccgcg
      241 ggcgttgtcg acttcgatgg ctatgatatg cggatcgtcg gtgcgcgccg cgcgaaacac
      301 gccgttctgg atcatgcgct gcaccgacaa ccgattgtcg ttggtcggcg tgacgtaatg
      361 caccaattcg gttttgtagc gatgcaacag gaaaaaatgc atcaacgtca tcaaccgccg
      421 ggaacggaat ttcttaacgg tattctggtc cttgaccgcg aggaacttgc gtccggcacg
      481 atccttgtcg gcgcgaaaaa tgatctcggc gattttgtcg tgggcctcgt cgaacaggtt
      541 aagcatcatg tcttcggagc cggcccagcg ctctttcaat tcgacgctca aggcgccgcc
      601 ctgataaagg ccctgccggt gccagtgctc ggcccacatg gccagccatt tcaccagcac
      661 gctggtggga acctcgaccg ctttgcgatg ctgggtttcc gtcgatttcc cgcccatgga
      721 ggagctcgtc agcgacgcgc cgcgcgcggc gcgccggaaa cggtcggccc agtcgccgcc
      781 gacaaagctc tggggaaatt gcgccggatc atgggccagg cgcagcgcgc gctgaatcaa
      841 tcggacaaag ccgagcacgc cttcttcttt caacgcccgc gccaagcggc cgttcatcag
      901 cgcctcagtt tggtggccgc cgtaagtgat gaagttgaag acgtagccca tttttcccag
      961 ctcgctggca aaattgcgaa tctgatcgtc ggtaaatccc caaacgtccc aattccacga
     1021 cggcgacaag ttgtacgcca acattttttc cgggtgcacg gcgtggatct catcggccca
     1081 ggctttatcg tcggccagat cgggcttcgc cgtctcacgc cagatcagat cggcgaaggg
     1141 ggcggtggcc aatcccctgg cggtagccat ggcgcggccg ccggtgatca catagaaacc
     1201 ttccggtgtt ctcggcaggt cccaatccca gaagatatca acgcccatcg ccgcggcgcc
     1261 ggcgcgcgct tcggcatggg acaagccggc ggcaaacttc ttccagcgct cggcggacat
     1321 cgcgagcttg ccggcgccgc tcgccaacac ttccgccacc gcttcggcat aggtcttaag
     1381 ccgggctttg tccgcccact cctggcggat cccggcaacg acctcgtcga cggtttcttc
     1441 gatgactttc ttcacagcga tttcctgtgc cgccaactgc tcgcgaaagt cgcgctggcc
     1501 cttgcccggc cggcgcaccc gctggcgcaa tttttttagg gcgacgatct cgctgtcgat
     1561 tctatctata cccgcctcaa tgcggtcgag gagccgctcc tgccggagcc acttttccgc
     1621 gccggcgtaa gcttgctcgg aaatcttgta gagcaggtgg ccgttaatct ccttgaagcc
     1681 cttttggtaa aacttgcgaa tcaccgccag gctgacattc ttgaacggca cgatgtcggg
     1741 attggtcgca ccgtagacaa agggatggtc gcgatcgtcg tcgacgctgt cgatggcggt
     1801 ggcgtcgttg gagtcggtgc gggcgacgat cacgcccggc acatgcatga tgtcgtattg
     1861 cagccgcgcg gcgttgagac gggaaatcat ttcggaggtc gacactagga ctttttgccc
     1921 ctgatgaccg caaaccttgc tgcccgcgcg ttggtcttcg atatggattg cgccgatgtt
     1981 gttttcgacg aaggtcttga ccaggttgcg cgtatggtgc tcgccaccgt ggccggtgtc
     2041 gccatcggga atgatcagcg gcgggctaaa ctcgatggcg cgagtctttt tgcgctgcag
     2101 cgacgacatg cgaatgcgcc gcgaacgctg cacctcgtct tggtgcaaga gcgcgcgcac
     2161 ccaggcgccg ccttctttgg gcacgcgatc cagcgcatag ttggccaggt cggcgcccgg
     2221 gtcttcggct tcggagccct tggccgaagt cgcccagccg ccgagataaa gcaccttgat
     2281 tccctccatg accgcgcgca ccgcgccggt gggcgaatac gggccatagg tgatctcttg
     2341 ttttttgtcc cggaacagcc gctgcaagta atcgaagagc ttgagcgccg attgtttggc
     2401 aatcgtatgg tcggcctgca gattgccacg caaggaaacc acctcggccg cagtatgcag
     2461 acgggtaata tgccggaaac gcgaactgtg aaaccagccc tcggtttcgc gcagttcctt
     2521 ggcccatgcg atcgcctcga cgtcccggcg tgaatccaag cgaatgattt tgtcagccat
     2581 agcgcagccc tttttgttgt cggtctaaat cgacgatttc ccccaatgcg gcttacctga
     2641 aaggggtgcc taagctaata aaaagtgatc gtctaatcaa gcgccggttt tggccgctgg
     2701 ccggttttct tgaagtgaaa gtaatcgcgc aggatgaccg cgtggtcgaa tgccagcggc
     2761 gccggcaaat tattttcaaa aaatattttt gccacccgcg catcgtcagc gccccgcggc
     2821 gttccctgcg ccgtagcgat aaacaccacc gccaatgtgt ggtggcgcgg gtcgcgccgg
     2881 ggatcggagt aggtgtagaa ctgctccgtc aagtttacct ctaagcaggt ttcttcccgg
     2941 gcttcgcgca ccgcggcgcg ctccacgctc tcgccgtaat cgacgaatcc gcccggcatt
     3001 gcccaacccg gtggcggatt ttttctttcg atcaagacga tgccgccggc aatctcgatg
     3061 atgatatcga cggtgggaaa aggattgcga aatttttcat tcatggcgct tccctacctt
     3121 gactccatga aaagcgcaag gtagaaagac cactcaacaa tttatcgcgg ggtggagcag
     3181 tctggcagct cgcaaggctc ataaccttga ggtcccgggt tcaaatcccg gccccgcaac
     3241 cacaacaacg caaggaggct ccatgggcct cctttttttt gggtcaaggt caggctttat
     3301 cgatgcgccg acgttgaagg gacttggccc tcgattggac cagttgcttg cttggggtaa
     3361 attaatgaaa agcaatcgac ccgtttcaag gaagatagtc gatcatgaaa atcatgattc
     3421 catatgcctt cttaatcttg gtctttcttc ttactccggc agcagcatca aaccactaca
     3481 cggaaaagca acttaacgcg ctcgaggcgc gagtcggcaa aatgttctgg atatcaccag
     3541 tcgaagaaaa gctcccgtct tttgttacat ctccagcttc tagcgcagcg gcattccaac
     3601 cttcggccaa tgaatccttc gagatcactg agctggtcgg gcgaaaagcc aagaatcctt
     3661 attacaaagt aaagtttgaa tcagggaaag agggttacat tcacgctcaa gcgtttcacg
//
LOCUS       DASQKS010000496         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_694041, whole genome shotgun sequence.
ACCESSION   DASQKS010000496 DASQKS010000000
VERSION     DASQKS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694041"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1147
                     /locus_tag="VE170_12815"
     CDS             287..1147
                     /locus_tag="VE170_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ16368.1"
                     /translation="MLSNKVSEIMTTELVSAAAESTVHEVIAQMVARDVGRIIIIRDD
                     LPVGIFTERHVLKHVVNSKLDPQQTPVEKVMSSPFRAVAEDTHIVEALGEMIKGKIRH
                     LQVLGTRDNIVGIVSMRRILEIAVELGHDLGETQTIGSIVTQTPLSMDESESVWSAVE
                     IMNRNAAGAVVVTSTLKPVGLFTERDVLNRVVAKQLDGTNTVLKQVMTSPLILMPDTA
                     LVGQVLGEMYRRDIRNMPIVDASDRLAGLVAMSDVLQYARAFDIDEEVRRTWKEIRTF
                     YDSQDNYTPG"
     gene            1434..2690
                     /locus_tag="VE170_12820"
     CDS             1434..2690
                     /locus_tag="VE170_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16369.1"
                     /translation="MTGKLTAHFNGLFYGWRMVAAGCAMRMLGGGFHLYGFTIFFLPI
                     TNELGLSRAATSLVFSLARAEGAIEGPLAGYFIDRYGPRPMMLAGIILSGLGYMLLAT
                     VHSYAAFLAVYLGVISLAFSAGFMHSPMVLANSWFIRRRAMAMTLISSAIGIGGTLIT
                     PILAFTVAQWGWRYGAFITGIGLIVTGVPVALYVKRSPESMGLVPDGVSIEVSEQDNR
                     KTPSATEREFSLKQAMKTAAFWKLILATTTRVGVFNSITVHFVPIMVWRGASEAKAAA
                     LLATLALMSLPSHLFVGWIADRVNKPRLMAACMVIGAGAVAILAYGVGEWALWTFTLL
                     FTFVEALFPVSWATVGDYFGRKHFATIRGSMSFFYLWGPALGPVITGYVYDRHQSYAP
                     LMSAYIAIALVAGLLYASLRQPKLPA"
BASE COUNT          558 a          783 c          748 g          603 t
ORIGIN      
        1 tttatgacaa aatccatctg gcagaatcgc ggcgctggcg ggcatgaccg ctgcctttgc
       61 ccgcgtcggc tgccagggtt tcggttgagc tcccaggaac ctaacagacc ttcttgtgat
      121 gaaaccactc cgaataaaaa atctctactt gattaatctt gacggagtgc attaaatcaa
      181 aatatcacta gcgggaaaga ttgtcgcgtc acgaaaacaa ccggtaaaga caaacttgag
      241 acccgacaaa ccgatacgat gaaatccggt ttcgcggagg tgctgcatgc tgtcgaacaa
      301 ggtcagcgag ataatgacca ccgagctcgt atcggctgcc gccgaaagca cggttcatga
      361 agtgatcgcg cagatggtcg cccgcgacgt cgggcggatc atcattattc gcgacgatct
      421 cccggtgggg atttttaccg aacggcatgt gctcaaacat gtcgtgaata gtaagcttga
      481 tccgcagcaa accccggtcg aaaaggtcat gagctcgcct tttcgtgcgg ttgccgaaga
      541 cacccatatc gtcgaagcgc tgggcgagat gattaaaggg aaaattcgcc atcttcaggt
      601 gttagggacg agggacaaca tcgtcggcat tgtttcgatg cggcgaattc tcgaaatcgc
      661 cgtggagctc ggtcatgatc tgggcgaaac tcaaaccatc ggcagcatag tcacacaaac
      721 cccgttgtcg atggacgaat ccgaatcggt ctggagcgcc gtcgagatca tgaatcgaaa
      781 cgccgcaggc gccgtggttg tgacctcgac gctgaagcca gtgggacttt ttaccgaacg
      841 cgacgtgtta aatcgggtcg tcgccaagca gcttgacgga accaacacgg tacttaaaca
      901 ggtcatgacc tcaccgttga tcctcatgcc ggacaccgcc ctggtcggac aagtcctcgg
      961 ggaaatgtat cgccgggata ttcgcaacat gccgatcgtt gatgcatcgg atcgactggc
     1021 cggcctggtt gcgatgtcag acgtgttgca gtacgcgcgc gccttcgata tcgacgaaga
     1081 ggttcggcgc acgtggaagg aaatccgcac cttctacgac tcgcaggata actacacgcc
     1141 gggatagtcg gccagcgtac gcttcgaatg actgaatttt tatcgcttgc atagtagcgc
     1201 cttctgatcc ttctcaattg catggtgcga tcaattggcg cggcgatctt ttgataccac
     1261 gacgatcgca ccggccggca tcgtccgtcg ggcccacgat aattttcaat gccaccgaat
     1321 gccatcccac gttgcttcat tgccgcgcag ccgcgttgac agacttcggg ccactcggat
     1381 aggttgaaaa aaagtcgcca cgccacagcc ttgcagcttt ttacccgaca ccaatgaccg
     1441 gcaaactcac cgcgcacttc aacggcctat tctacggctg gcgcatggtc gcagccggct
     1501 gtgccatgcg catgctcggc ggcggctttc atctttacgg ctttacgatt ttctttctgc
     1561 cgatcaccaa tgaactcggc ctatcgcgcg cggcgacctc cctggttttc tctctggccc
     1621 gcgccgaggg cgccatcgaa ggacccctcg ccggctattt catcgaccgc tacggcccgc
     1681 gaccgatgat gcttgccggc attattttgt ccggcctggg ctacatgctg ctcgccacgg
     1741 tgcacagcta cgcagcattt ttggcggttt atctgggggt gatctcgctg gcgttttccg
     1801 ccggcttcat gcattcaccg atggtactgg ccaactcttg gtttatccgc cgccgcgcca
     1861 tggcgatgac cttgatcagc agcgccatcg gtatcggcgg cactctgatc acgccgatcc
     1921 tcgctttcac cgtcgctcag tggggctggc gctacggtgc gtttatcaca ggcattggcc
     1981 tgatcgtcac cggcgttccg gtcgcgcttt acgtcaaacg ctcgcccgaa agtatgggac
     2041 tcgtgcccga tggcgtatcg attgaagtat ccgagcaaga taatcgcaaa acgccgtctg
     2101 ctaccgagcg cgagttcagc ctgaagcagg cgatgaaaac cgccgccttc tggaaactga
     2161 tattggcgac cacgacccgc gtcggggtgt tcaattcgat cacggtccac ttcgtgccga
     2221 tcatggtatg gcgcggcgcc agcgaagcca aagccgcggc gctgttggcg accctggctt
     2281 tgatgagtct gccgtcccac ttatttgtcg gctggatcgc cgaccgggtc aacaaaccca
     2341 ggctgatggc agcttgcatg gtgatcggcg ccggggcggt ggcgatcctg gcctacggcg
     2401 ttggcgaatg ggcgttgtgg acatttacac ttctttttac cttcgtcgaa gcgttgttcc
     2461 cggtgagctg ggcgacagtc ggagactatt tcggcagaaa acatttcgcg acgatccgcg
     2521 gcagcatgag ttttttttac ctctggggtc cggcattggg cccggtcatc accggctacg
     2581 tctacgaccg ccatcaaagt tacgcgcctt tgatgagcgc atacatcgcc atcgcgctgg
     2641 tcgccggctt gctctacgct tcgctgcgcc aaccgaaatt gccagcctaa aa
//
LOCUS       DASQKS010000497         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2386935, whole genome shotgun sequence.
ACCESSION   DASQKS010000497 DASQKS010000000
VERSION     DASQKS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386935"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VE170_12825"
     CDS             <1..647
                     /locus_tag="VE170_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16370.1"
                     /translation="YHFADRYSAADGYFNQGVKRYEEGMQHIWETNFISDIQTANVDS
                     REVKGAGVGITQFEIAGNSLIGHLATWPAARYHKAHYHGAGAILLGLQSSGYVLLWSK
                     EAGTHPFENGRGDEVVEVKWKAGSVYCPGGGWFHQHFNTGADPARHLALRYGSRIHPI
                     GFKIADKRSEDGVYIDVKQGGTLIEYADEDPYIRQHYDDELKKTGVKSAMPALK"
     gene            741..2084
                     /locus_tag="VE170_12830"
     CDS             741..2084
                     /locus_tag="VE170_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011770156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYJ16371.1"
                     /translation="MPESKLRDKFFRSRQFLKTDIWRIHARKLTRRELFFINPLRIVL
                     LAIRRFYDNKCELRASALTFYTLLSIVPVVAMAFGVAKGFGLEKILEAQLLAKLEGQP
                     EVAERLLGFARTLLENTKGGALAGVGVAVLFWSVVKVLGNIESALNDIWGIPTARPLG
                     RKLVDYLSVMLIGPVLLIIASSATLLVTTQVTTVIEGLAFLGYAAGMIVFLIKLLPYA
                     VIWFLFSFMYAFMPNTKVQLKSAICGGILAGTIYQLTQFAYIKFQIGVSGYGAIYGSF
                     AALPLFLVWLQLSWLIVLFGAEVSFAHQNVAVYEFAADCDSLSHRFKRTVALFITHHC
                     VKAFVQGQSAPTAEEIAQELEIPVHLVRSTLFELTNARILAEIRRSESEVVAYQPGSP
                     LDQLTITKVMIALDRQGADALPLADSPSLDKLREITTKFDEILEAAPANRKIQEL"
     gene            complement(2163..3287)
                     /locus_tag="VE170_12835"
     CDS             complement(2163..3287)
                     /locus_tag="VE170_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16372.1"
                     /translation="MTPRFRIYTVVAAIGFLVCLTAGVAIAQSDWKKDWERVQQEAKK
                     EGTLVVGIPARAELRKQMEAVFKPKFAIDMDLSTARGPQNASRIAAEYAAGVKNFDVF
                     IGGSGTFESLVEGGMVEPLDALMLLPEVKEAKQWWGGHIWEDNLSGNRHLYSFIAEAG
                     TGGLWFNTEAAKPNELRSFDDLLNPKWRGKIGFLDPRTPGSGQSVWSYLWDKKGEPFL
                     RKLLQQDLFISRDQRQLGDALAKGKVAVALGVSFYTLEGFILANLPVKELPTPKEGLP
                     SSNGSGVIGVVKNAPHPNAARLFVNWLLSKEGQELYVKAMHQSTRRLDVDTKWLREHG
                     VRAAKDNMTVDQYHKARNHLEDKYTRVRIPAGKFAEEIIK"
     gene            complement(3314..>3653)
                     /locus_tag="VE170_12840"
     CDS             complement(3314..>3653)
                     /locus_tag="VE170_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16373.1"
                     /translation="KCAAAVLSTSFMAVDSQMGFGRLTTKLVLTNAKELEPHKGRKID
                     FNVDMTFALLGLLSRIISPVGANFRCAKAKRAVFKPAPFDFRFFFGASAVFATFDFSV
                     CGFAVLGPSR"
BASE COUNT          795 a         1059 c          976 g          823 t
ORIGIN      
        1 cctatcattt tgccgaccgc tactccgccg ccgacggcta tttcaatcaa ggcgtcaagc
       61 gctacgagga aggcatgcag catatctggg agaccaactt catcagcgac attcaaaccg
      121 ccaatgtcga cagccgcgaa gtcaaaggcg ccggcgtggg aatcacccaa ttcgaaatcg
      181 ccggtaatag tttgatcggc catctggcca cctggcccgc ggcgcgctat cacaaagcgc
      241 actatcacgg cgccggcgcg attctcctgg gcttgcagtc ctccggttat gtcctgctgt
      301 ggtcaaaaga ggcgggcacg caccccttcg agaacggccg tggcgatgaa gtggtcgagg
      361 tcaaatggaa agccggcagc gtctactgcc ccggcggcgg ctggttccat caacacttca
      421 acaccggcgc cgatcccgcg cgccacttgg cgctccgtta tggcagtcgc atccatccca
      481 tcggtttcaa gatcgccgac aaacgcagcg aagacggcgt ctacatcgac gtcaaacaag
      541 gcggcacctt gatcgaatac gcggacgaag atccgtatat tcgccagcac tatgatgatg
      601 aattgaagaa aaccggtgtg aagagcgcga tgccggcgct caagtaattg ttcaagagtt
      661 caaaggttca aggattcatc ccgaactaga aaataacgag ccttgaaccc ttgaagcttt
      721 gaacggattc gaaacagacc atgccggaaa gcaaattgcg cgacaaattt ttcaggtcga
      781 ggcaatttct caaaaccgat atttggcgca ttcacgcgcg taaattaacc cgtcgagaac
      841 tgtttttcat caatccgctg aggattgtgc tgctggcgat ccgccgattc tacgacaaca
      901 agtgtgaatt gcgcgcgtcg gcgctgacct tttatacctt gctgtcgatc gtacccgtgg
      961 tcgcgatggc attcggagtc gccaagggat tcggcctgga aaaaatactc gaggcgcaac
     1021 tgctcgccaa attggaaggc cagccggaag tcgccgagcg cctactcggc tttgcccgca
     1081 ccctgttgga gaacaccaag ggcggcgccc tcgccggcgt cggtgtagcg gttctgtttt
     1141 ggtccgtagt gaaagtccta ggcaatatcg aaagcgctct caacgatatt tggggcatcc
     1201 ccacggcgcg ccccttgggc cgcaagcttg tcgattattt atcggtgatg ctgatcggtc
     1261 cggtgctgct gatcatcgcc agcagtgcga cgctgttggt aaccacgcaa gttaccacag
     1321 tgatcgaagg gcttgccttt ctcggttatg ccgctggcat gatcgttttt ctcattaagc
     1381 ttctgccgta cgcggtgatc tggtttttgt tcagctttat gtatgctttc atgcccaaca
     1441 ccaaagtgca gctcaaatcc gcaatatgcg gcgggatcct ggccgggacg atctatcaac
     1501 tgacgcagtt cgcctacatc aaattccaaa tcggcgtctc cggttacggc gctatctatg
     1561 gcagcttcgc ggcgctgccg ctctttctgg tatggctgca attgagctgg ctgatcgttc
     1621 tgttcggcgc cgaggtctcc tttgcccatc agaatgtcgc cgtctatgag ttcgccgccg
     1681 actgcgacag tctcagccac cgtttcaaac gcacggtggc tttgttcatc acccatcatt
     1741 gcgtcaaggc gtttgtccag gggcaaagcg cgccgaccgc cgaggagatc gcccaggaat
     1801 tggaaattcc cgtgcacttg gtgcgctcca cccttttcga gttgaccaat gcgcggatcc
     1861 ttgcggagat tcgtcgcagc gaaagcgagg tggtcgccta tcagccgggc tcccccctcg
     1921 accaattgac gataaccaag gtcatgattg cgctggaccg ccaaggcgcg gatgctctcc
     1981 cattggcgga ttcacccagc ctagacaagt tgcgcgagat aaccacaaaa ttcgacgaga
     2041 tcttggaagc cgcgccggcg aatcgaaaga ttcaagaact ttgagccgtt tgtttccaaa
     2101 tatcttgaaa gccttgacga tcccgaccta tctaaagcag cacatactgc aaacgccaca
     2161 gatcatttga taatttcctc agcaaacttc cctgccggta tgcgcacgcg ggtatattta
     2221 tcctccaaat gatttctcgc cttgtgatat tggtccaccg tcatattgtc cttggcggcg
     2281 cgcacgccgt gttcgcgcag ccacttggtg tcgacatcga ggcggcgggt cgattggtgc
     2341 atggctttca cgtataactc ctggccttcc ttggacagca gccagttgac gaacaggcgc
     2401 gcggcgttgg ggtggggcgc gttcttgacg acgccgatga cgccggagcc gttgctcgac
     2461 ggcaaccctt ccttgggcgt gggcaactcc ttcaccggca gattggcgag gatgaaacct
     2521 tcgagggtgt agaagcttac gcccagggcg acggcgactt tgcctttagc cagggcatcg
     2581 cccaattggc gttgatctcg gctgatgaat agatcctgct gcaagagctt gcgcaagaag
     2641 ggctcgcctt ttttatccca caaataagac cacaccgact gccctgagcc cggcgtgcgc
     2701 gggtcgagaa agccgatctt gcctctccat ttggggttca gcagatcgtc aaaggaacgc
     2761 agctcattgg gcttcgcggc ttccgtgtta aaccacaagc cgccggtgcc ggcctcggcg
     2821 ataaatgaat agagatggcg gttgccgctc aagttgtctt cccagatatg gccgccccac
     2881 cattgtttgg cctctttgac ctcgggcagc agcatcagcg catccaacgg ttcgaccata
     2941 ccgccctcga ccaacgactc gaatgtgccg ctgccgccga taaagacatc gaagtttttc
     3001 actccggcag cgtattccgc ggcgatgcgg ctggcgtttt gcggtccacg cgccgtcgac
     3061 agatccatgt cgatggcgaa tttgggtttg aacaccgcct ccatttgttt tcgcagttcg
     3121 gcccgcgccg gtatgccgac gacgagggtg ccctctttct tcgcttcctg ctggacccgc
     3181 tcccagtcct tcttccaatc gctctgcgcg atggcgacgc cagccgtcag acaaaccaag
     3241 aaaccaatcg ccgcaactac cgtgtagatt ctgaacctag gtgtcatttt aactcctatt
     3301 gcaaaagact tgttcatcga gaaggaccca gaacggcgaa gccgcaaacc gaaaagtcga
     3361 atgtcgcaaa gaccgccgag gcgccaaaaa agaatcggaa gtcgaaggga gcgggtttga
     3421 aaaccgccct cttagctttg gcgcatcgaa aatttgcccc aaccgggcta attattcgag
     3481 ataatagtcc gaggagcgcg aaggtcatat caacgttgaa atcaattttt cgccctttgt
     3541 gcggctccag ttccttcgca ttcgtcagga caagctttgt ggttaaccgt ccgaatccca
     3601 tttgactatc tacggccata aagcttgtcg ataaaaccgc tgctgcgcat ttg
//
LOCUS       DASQKS010000498         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6160945, whole genome shotgun sequence.
ACCESSION   DASQKS010000498 DASQKS010000000
VERSION     DASQKS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6160945"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..976)
                     /locus_tag="VE170_12845"
     CDS             complement(77..976)
                     /locus_tag="VE170_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16374.1"
                     /translation="MADTKPIVLERIDHGVLPSNDLGRSFRFWSSFMGGQIGHHTNLT
                     VRGLNREVPMIIFMTVANHRGFGLALQDFRISPNPARRLEGVVWGFEVAADNLAAASA
                     EAEKQKLRYERVADYPASSPVKESLFVLDPDGNTVELCIRKQPSDKAPQAGPLPLRRI
                     SHVRVEVTDLEQARDWYANTFGLVVGEQVPGEDQLTMTVAKSNQLVILRNVDKVADRS
                     AQCYKGPHIDLRSDEASYPEMLRRFDRKETYWGPDPTLIPWHEPDTNTAYGYDPFGNR
                     IQIGIIAKRPMHHGDVARFRQQP"
     gene            1084..2094
                     /locus_tag="VE170_12850"
     CDS             1084..2094
                     /locus_tag="VE170_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16375.1"
                     /translation="MKLKFSLPTMVFIFVAAVGPAGLLAQVPSETLRVSFASFGVIYY
                     PHFVAKELGYYRDEGLNVEVIAMPGGLATQALVAGDLHFSTSSGSSLNASLRGIKLKV
                     VYVNLDRPLYKILSWRDDIRKVPDLRGKGIGIASRGDTMEGAANLMLREYNLDPLRDV
                     IWIALGTGGRVTSLAAKAVDSAVLGFADSLRMQTRGFAVHEVVNIGKEIKMLYTGLAT
                     TEETVVKRPDLVRRFLRATVKGREFVKRNKAQALALGKKYDKAPDDVRNADYDATMEM
                     MTADGTEDAETQKSDIEIAKRMLNVQHEVPVEQIFDFRFTREVYQELRALGWERALKA
                     AK"
     gene            2160..>2572
                     /locus_tag="VE170_12855"
     CDS             2160..>2572
                     /locus_tag="VE170_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16376.1"
                     /translation="MQIGLLLHTRQLIRADEAARSFEHLWVDAAQAEELGFDHIWLGD
                     SVTVLDKARGDCLTTMAALAAHTHKIRLGIVPMLPALRNPVLLAHALATIDMISNGRI
                     ILGVSVGPVRDYIQRQFTACGVPPQEKAGRLSETI"
BASE COUNT          559 a          737 c          746 g          530 t
ORIGIN      
        1 gtccgagagt tcggaattcg cttttgagaa ctttgcgttc tttgcggcta ccgatccgat
       61 ctcccacttc gcctacctat ggctgttggc gaaaccgcgc gacgtcgccg tgatgcatcg
      121 gccgtttggc gatgatgccg atctggatgc gattgccgaa ggggtcgtag ccgtaggcgg
      181 tgttcgtatc gggttcatgc cagggaatca aagttggatc tggcccccag taagtctctt
      241 tgcgatcaaa gcgccggagc atttccggat aggacgcttc atccgagcgc agatcgatat
      301 gcggtccttt ataacactgc gcgctgcggt cggcgacctt gtcgacattg cggagaatga
      361 ccaattgatt cgacttggcc accgtcatcg tcagctggtc ttctcccggc acttgttcgc
      421 caacgacgag cccgaaggtg ttggcatacc aatcgcgcgc ttgttcgaga tcggtcacct
      481 cgacccgcac atggctgatg cgccgcagcg gcagcggacc cgcttgcggc gctttatccg
      541 agggctgttt gcggatgcat aactcgacag tgttgccgtc gggatcgagg acgaagagcg
      601 attccttgac cgggctcgat gccggatagt cggcgacgcg ttcgtaacga agcttctgtt
      661 tttccgcttc ggcgctggca gcggcgagat tatcggcggc gacctcgaag ccccagacga
      721 ccccttccaa acggcgcgcc ggattcggcg agatgcgaaa gtcctgcaac gccaggccaa
      781 agccgcgatg attggccacc gtcataaaga taatcatcgg tacttcacga ttgagaccgc
      841 gcacggtcaa attggtgtga tggccaatct ggccgcccat gaatgacgac caaaagcgaa
      901 acgaccggcc gagatcgttg gacggcaaga cgccgtgatc aatccgttcc aaaacaattg
      961 gcttggtatc tgccatggca aaactcctca aactgcgtgt atagcgcagc tgtgccggcg
     1021 agacaagcaa ccgtcgcgac cttgcgtttg agtgcgttcc acaggtatgt aaatcatact
     1081 gcgatgaagc tgaaattttc gctgccaacc atggttttta ttttcgtagc agccgtcggg
     1141 cctgctggtt tgttggcgca agtgccaagc gaaacactgc gtgtctcctt cgccagcttc
     1201 ggagtgattt attacccgca tttcgtcgcc aaggagctgg gctattaccg tgacgagggt
     1261 ctgaacgtcg aagtcatcgc catgccaggc gggttggcaa cccaggcgtt ggtcgccggc
     1321 gatctccact tcagcacgtc ttcgggttcg tcattgaatg ccagcctgcg cggcatcaaa
     1381 ctcaaggtcg tttacgtcaa tctcgaccgg ccgctgtata aaatcctcag ttggcgcgat
     1441 gacattcgca aggtgccgga cctgcgcggc aaaggcatcg gcatcgcctc acgcggcgac
     1501 accatggagg gcgcggcgaa tttaatgctg cgcgaatata atctggaccc gctacgcgat
     1561 gtgatttgga tcgcgctcgg caccggcggc cgggttacct cgctagcggc aaaggccgtg
     1621 gattccgcgg ttttgggctt cgccgactcc ttgcgcatgc agacgcgcgg ctttgccgtt
     1681 cacgaagtag tcaacatcgg caaggaaatt aagatgctct acaccgggct cgccacaacg
     1741 gaagaaaccg tcgtcaagcg accggacctg gtgcggcgat ttttacgcgc gacggtaaag
     1801 gggcgcgagt tcgtcaaacg caacaaagcc caggcccttg ccctcggcaa aaaatacgac
     1861 aaagcccccg acgacgtgcg caacgcggat tacgacgcca ccatggagat gatgaccgcc
     1921 gacggcacag aagacgccga aacacaaaaa tcggacatcg agatcgccaa gcgcatgctc
     1981 aacgtgcagc atgaagtgcc cgtcgaacag atcttcgact tccgcttcac cagagaagtt
     2041 taccaagaac tgcgcgccct cggctgggaa cgagcgctga aagcagcgaa ataaatgatt
     2101 aacacacgag gcgttctgag ccctttattt gtgacgatta tccgggaaat tggattacca
     2161 tgcagatcgg cttactgtta cacacacggc aactgattcg tgccgacgag gccgccagga
     2221 gcttcgaaca tttgtgggtt gacgcggcgc aagccgagga actgggcttc gatcacatct
     2281 ggctgggcga cagcgtcacg gtcttggaca aagcgcgcgg cgactgtttg acgacgatgg
     2341 cggcattggc ggcgcatacg cataaaattc gcctaggcat cgtcccgatg ctgccggcgt
     2401 tacgcaatcc ggtgctactc gcccacgccc tggcgaccat cgatatgatt tccaacggca
     2461 ggattatttt aggcgtcagc gtcgggccgg tgcgcgacta tatacagcgc caatttaccg
     2521 cttgcggcgt gccgccccag gaaaaggccg gaagactcag cgaaaccatc ga
//
LOCUS       DASQKS010000499         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3223016, whole genome shotgun sequence.
ACCESSION   DASQKS010000499 DASQKS010000000
VERSION     DASQKS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223016"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1009
                     /locus_tag="VE170_12860"
     CDS             38..1009
                     /locus_tag="VE170_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16377.1"
                     /translation="MTSKLFVVIVIVWMTLATLTTEAPAGQAIQPINLATSASGQSGY
                     VAALIENKGLDVKYGIKITNMMMDFTEAANAVKLGRAVASAMQPSTAVNLIKSGTDIR
                     LITPQIWSGNSWVVKKDAPYKSFAELKGKKIGNFARVTGAYFFAAVIAKEHKLDLEKD
                     FQNIPAETGALIALLERGDVEAINMFEPHVTRMVMSGRYRVLVDFDIALEEIFGSPPL
                     KSTVGMLKETVEKQPQLVKALRGAYGDAVQMIKKGQDDEFFKSKAKEFFNLSSPEEVS
                     AGMRVNKENFSDKWGDAFFQSQNKILQSGVGLGLLPNIGDLNALWIK"
     gene            complement(1166..2020)
                     /locus_tag="VE170_12865"
     CDS             complement(1166..2020)
                     /locus_tag="VE170_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ16378.1"
                     /translation="MAAEKTLTWPDDARIAFVLGIAFEAWDRDKPTQGAALQSPLPKD
                     ALWKRDWSTETFREFGAKVGLKRLLDICDRYAVKTSVQVNGLTCVYYPELVKDCYDRG
                     HELVAHGWDQGERLYMLTREQERENILKTIDAIVKVTGERPKGWSSPGVRPTNNTVEL
                     LAETGMEFHCDFHDDEMPYPIWIGDKLIIEFPHQYTINDHRMQEEGTRQEFFEKFTDE
                     FDYRYRIGDRNPTMMNVITHAYLISRIPFIDTFEKIISYAKNHKDVWFARRGDVAAWT
                     RKHVLARK"
     gene            complement(2204..>2611)
                     /locus_tag="VE170_12870"
     CDS             complement(2204..>2611)
                     /locus_tag="VE170_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16379.1"
                     /translation="QQYPVGEILQFEAELHALRKEDRQIPEPYGPSQILRGVAGYLDK
                     REGCRLVAVSVKDRWVTINYLARDGRPEKATQDFEYFYDYGIKMFMQRSNRGKLPPPS
                     DPTLFVTWEGAQKRHQLSRMPAQARKVDTPNTG"
BASE COUNT          614 a          756 c          691 g          550 t
ORIGIN      
        1 tcgaaaccaa agtgactctc aaagtggagg atgaacaatg acaagtaaac ttttcgtcgt
       61 aatcgtaatc gtttggatga cgctcgccac cctaacaacc gaagccccag ccggacaggc
      121 gatacagccc ataaacctcg ccaccagcgc gtccggccag tccggctacg tcgctgcgct
      181 gatcgagaac aaagggctcg acgtcaagta cggaatcaag atcaccaata tgatgatgga
      241 ctttaccgaa gccgccaacg cggtcaaact cggccgcgcc gtcgccagcg ccatgcagcc
      301 gagcaccgcg gtcaacttga tcaagagcgg caccgacatt cgcctgatta cgccgcagat
      361 ctggtccggc aactcttggg tggtaaagaa agacgcgccc tacaagtcct tcgccgagct
      421 caagggcaaa aagatcggca atttcgcccg ggttaccggc gcgtactttt ttgccgcggt
      481 catcgccaag gaacataagc tggatctcga aaaagatttt cagaacattc cggcggagac
      541 cggcgcgctg atcgctctac tcgaacgcgg tgatgtcgag gcgatcaaca tgttcgaacc
      601 ccatgtcacg cgcatggtca tgtccggccg ctaccgcgtg ctggtcgact tcgatatcgc
      661 cctggaggaa atcttcggct ccccgccgct caaatccacc gtcggcatgc tcaaggagac
      721 ggtcgagaaa caacctcagc tggtcaaagc tctgcgcggc gcctacggcg acgccgtgca
      781 aatgattaaa aagggacaag acgacgagtt cttcaaatcc aaagccaagg agtttttcaa
      841 tctgagtagt cccgaagaag tcagcgccgg catgagagtc aataaagaaa acttttccga
      901 caaatggggc gacgccttct ttcagtcgca gaataagatt ctccagtccg gcgtcggctt
      961 aggcctgctg ccgaacatcg gcgacctcaa cgcgctgtgg attaagtagc tcaagaaacg
     1021 accgagccgc gaccccaact atccctctcg gctcggcgtc tatccgcctc ccctgacgat
     1081 ttgaacgggt tgaacgtttt gaacgattgg aacgaaaagt cgaggcaccg cgttaactca
     1141 ccgtaaaagc gtgaaagtta aaaacctatt tacgcgctaa cacatgcttc cgcgtccacg
     1201 ccgcgacatc gccgcgccgg gcgaaccaaa catccttgtg attcttggca taactgatga
     1261 tcttctcaaa agtatcgatg aagggaatcc gcgaaatcag ataggcgtgg gtgatgacat
     1321 tcatcatggt cggattgcgg tcgccgatgc ggtagcggta gtcgaactcg tcggtgaact
     1381 tctcgaagaa ctcctggcgt gtgccttcct cctgcatgcg atgatcgttg atggtgtatt
     1441 gatgggggaa ctcgatgatc aatttatcac cgatccagat cggatagggc atctcgtcgt
     1501 cgtgaaaatc gcaatggaat tccatgccgg tttcggcaag cagctccacc gtgttattgg
     1561 tcgggcgcac tcccggcgat gaccagccct tgggccgttc gccggtgact ttgacgatgg
     1621 catcgatggt cttgaggatg ttctcgcgct cctgctcgcg ggtcagcata tagagccgct
     1681 cgccttgatc ccatccatgc gcaactaact catggccgcg gtcgtaacag tccttcacca
     1741 gctccggata gtagacgcag gtcaaaccgt tcacctgcac gctagtcttc acggcgtagc
     1801 gatcgcagat atcgagcaaa cgtttcaggc caactttggc gccaaattcg cggaacgtct
     1861 cggtcgacca atcccgtttc cacaaagcat ctttgggcag cggcgattgc aacgccgccc
     1921 cctgggtcgg tttgtctcta tcccaggcct caaaggcgat ccccaagaca aaggcaatcc
     1981 gggcatcgtc gggccaagtc aaagtttttt cagccgccat gcagttttct ttccggaccg
     2041 ccggtcgctc tccgaccacg gacgcatcgc gaccataaca agtgcgattt tctcttgtca
     2101 attatcggaa agagaaagtt aacaaaaatc ctagcgtcga tacccgcgct gcgaatcgcg
     2161 gaacggacca accgggacta aatcacgaca ggtcaacgaa ggatcagccg gtattgggag
     2221 tgtccacttt gcgcgcttga gcaggcatgc gcgacaactg gtggcgcttt tgcgcgcctt
     2281 cccaggtcac gaaaagtgtc ggatcgctgg gcgggggcaa tttaccgcga ttgctgcgct
     2341 gcataaacat cttgatcccg taatcataga aatattcgaa atcctgagtg gctttttccg
     2401 gtcggccgtc cctggcaaga tagttgatcg tcacccagcg atccttcacg cttacagcta
     2461 cgagccggca accttcgcgc ttgtcgagat agccagcgac gccgcggaga atttgcgacg
     2521 gaccataggg ttccgggatt tggcggtcct ctttgcgcag ggcatgcaac tctgcctcga
     2581 attgcaaaat ctctccaacc gggtactgct g
//
LOCUS       DASQKS010000500         7875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_253021, whole genome shotgun sequence.
ACCESSION   DASQKS010000500 DASQKS010000000
VERSION     DASQKS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253021"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(497..2047)
                     /locus_tag="VE170_12875"
     CDS             complement(497..2047)
                     /locus_tag="VE170_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16380.1"
                     /translation="MDLPIWDEAIYVGQGARFLHGDTLGAIFISPVYNLLYSSLIKIF
                     GSIQSVFYMQYLVKITVSTLLLLTLVEHLRSRSLALLLTLIWVASGVNIFERVLVYHV
                     ALGLFLLALFFLNKHQGIALLLLCLSSLTRLEYVFPTLAFAAFLTYMAVSNRKNKRFE
                     FVLQKIRMTLPVFVALIITMLIVYVILNVDDLNPGTKRTWLAFRQNYSLHEVESGRFQ
                     LNPYLDYNYVMQNDFPGAKSLLDAFLINPKLFLHHVLRNIAMLPIAILLFGIPYIGLK
                     IWGLVYGVLLGFAVTIMTQAAVLNPRFLVSRLLRVILERKDLLYVTLASMLSLIPILL
                     VYPKPHHTLIMVPICLLWVGLACLQVLNIINFPQFSRWSLTTLNILVILSILITSKPY
                     ASQRPERPVYEKITQLIELLPRDKIKLMGVGASWYVSYLPIGRVDAVEPLAVAGEDTK
                     NNRSDLRVLLKLHNPDAVIINRELVTSKNFDVGSLEDLKSDRWIKYPIGVDSVYFLRE
                     KLKNTHER"
     gene            complement(2368..2443)
                     /locus_tag="VE170_12880"
     tRNA            complement(2368..2443)
                     /locus_tag="VE170_12880"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2408..2410),aa:Ala,seq:ggc)
     gene            complement(2586..4283)
                     /gene="recN"
                     /locus_tag="VE170_12885"
     CDS             complement(2586..4283)
                     /gene="recN"
                     /locus_tag="VE170_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYJ16381.1"
                     /translation="MLRELRIKNFAVIDEVALELGPGLNIITGETGAGKSIILNALGL
                     VSGERGSSEIIRHKEEEATVEALFDGVPKPIERKLADAGFELDGELVVKRILNRSGKN
                     RIYLNGSLCSLNVLADIGGSLVHIYGQHEHHSLLQPETHLNLLDAFADVDAMTLAMKQ
                     QFVALTAAWSDLNETRDSLDRRRRERANLASQVEEIARAQLKTGEEEELRARKNILAH
                     SEKLTLGCREGEDLLYEGDAALVGRLGKYAAKLRELAGIDANLQPTEELIQSSLAQLQ
                     EAAAQLRRYAERVHFDPRALEQLEDRLAEIQRLKRKYNASIDDILRMEGDIQQSLERL
                     ENSEEQIAAMEKTFAAARGSAWNSAEKLSNERRRSAKKLRRELEKEARGLGMAEVAFE
                     ARFLSQDEKADVPPFFIAGKRLTERGMDHVEFYFSSNPGEPVKPLAKIASGGELSRLM
                     LALKSLVMTPGVVSTLLFDEVDSGVGGRVAEIVGKKLKQVAAQNQVISVTHLPQIAAL
                     ADCHYVVQKEVEKGRTFTRVHKLSEKDRIAEVARMLGGIKITEQTRKHAEEMVRGRD"
     gene            complement(4296..5147)
                     /locus_tag="VE170_12890"
     CDS             complement(4296..5147)
                     /locus_tag="VE170_12890"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYJ16382.1"
                     /translation="MIRTVGIVAKYHEPKAAETVRWLIPWLRKRGKKVCVENGLTPRG
                     AQSCTKKEMATQADLIISLGGDGTLLNIAPLVERPEVPILGVNMGGLGFITEVAVDEL
                     RSVLGKTLAGDYGVEKRMTLEVRVISKKGRLRRFRVLNDAVITKGARSRIIDLETYIG
                     DDYLCTYRADGLIISTPTGSTAYSLAAAGPILEPTLGAIVLSPICPHTLTNRPIVVPS
                     DAPIHVTLRSFGDTVILSPDGQKGLRLNNGDKVEVRDFGLPVSLIKLPSRSYYEILRE
                     KLKWGER"
     gene            complement(5411..6349)
                     /gene="phnE"
                     /locus_tag="VE170_12895"
     CDS             complement(5411..6349)
                     /gene="phnE"
                     /locus_tag="VE170_12895"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HYJ16383.1"
                     /translation="MAADRNQEIACDIMNEAPVRPHRSGRFLVFLGILLAVLVWSFHG
                     AKIRPGELIEGVPQIGQTLARMLPPDFSKITDAKHYYFPEQLSLGELLLPAPLAEADA
                     RARQRWWDNTFPQTVMGATLETIQMALAGTFLALLVAFPLGFLAARNTTPHPAVYRGV
                     RLLLNLVRTIPDLALGLLFVAAVGLGAFAGTIALAIHTATVLGKLLSESVENIDEGVV
                     EAIRATGAGYSQILSFAVVPQILPDLISFTLYRLETNIRAASVLGLIGAGGIGYLMNT
                     SFRTFQYQEAAAIVLVLIALVMLVDYCSSRLRRLVV"
     gene            complement(6636..7589)
                     /locus_tag="VE170_12900"
     CDS             complement(6636..7589)
                     /locus_tag="VE170_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16384.1"
                     /translation="MIAMQKALVLIWSAAFFFLVSLVGAASAQDAIAVITELKFNRGD
                     IQLRATNGAAATKPAVLQSLYTGNVVQTTKDALAVIFYTDGSRVVTVDEKSPIFEARA
                     SSAKDSAAGARVKEVANLLVGKKKPPTYVALSVRGKAQPPTLLSPRNSAILSDSPIFH
                     WTGTDQQPVTVKIFGQQGEIWSADNINLTKIAYPVTAPRLQADVEYGWSIERKGVAAS
                     KTTFKLLGGLEAQAVKERLAELGAMGRASKTTLAVLRANLLMSKQLFHEARDILTEAI
                     SADSDEPTLHFVLGELYDKIGLKNLATDEYNEAEYLAKAKP"
     gene            complement(7590..>7875)
                     /locus_tag="VE170_12905"
     CDS             complement(7590..>7875)
                     /locus_tag="VE170_12905"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ16385.1"
                     /translation="RLRIGVGIHSGPVVIGVVGTGERKKYDVTGDTVNTGSRVEGLNK
                     QYGTTILITRETMGQLKGQFVIRPCGTVQVKGRAQAVEVFEVRAAGAVGG"
BASE COUNT         1874 a         2183 c         2013 g         1805 t
ORIGIN      
        1 ttttgggaca gagcgctttg ccatcgaata gcattgatct gtggcggtac agtccgtcct
       61 tgaaacaagt ccgctatact ttgctttctg tccggagctt cttcgatgta aagccgcaaa
      121 agagtcacat gatcgatgcc gcaatccttg gcgcggacag aaatgtcgta ggcgggcatg
      181 aaacctaatt cccccgtcca aactcacacc gtcaatacca gtggttatgc aagatcgaac
      241 atacttcttc tagcgacgac gcacggggcc atttttaaac agcggagcga gagaagggag
      301 atgcgaggcg accgctggtc aggttgaagt atgttcggat cgtgctcgat aagagctatt
      361 ggactcgcaa cctgtgcgag acattacgcc agggaaaaac cgcatcgtcg acatagaaat
      421 caacgcgcac cgctgatggg atgtgtgctg cataaggcgg ccctacacgc tgccttaaaa
      481 tcacatggca acatcatcat ctttcatgtg tattcttaag cttttccctt agaaagtaga
      541 cactatcgac tccgattgga tatttgatcc accgatcaga cttcagatct tcgagcgaac
      601 cgacatcaaa atttttcgaa gtaaccagtt ctctattgat gatcactgca tctggattat
      661 gtaattttaa taagacccgt aaatcgctgc gattattttt tgtatcctca ccggccacag
      721 ctagtggttc aacagcatcg accctgccaa tgggtagata acttacatac caagaggcac
      781 ccacccccat aagctttatt ttgtccctcg gcagcagctc gatcaattgc gtaatctttt
      841 cataaaccgg gcgctcagga cgttgggagg cataaggctt ggacgttatt aaaatagaaa
      901 gaatgactaa tatattcaga gtggttaggc tccagcgaga aaattgcgga aagtttatga
      961 tgtttaatac ctgtaaacat gcaagcccca cccacagcag gcaaatcgga accattatca
     1021 gtgtgtgatg cggttttgga tataccagaa ggatggggat taacgaaagc atgcttgcca
     1081 gtgttacata caggaggtcc ttccgctcta gaataacccg cagcaagcgg gacacgagga
     1141 accggggatt aagaaccgcc gcctgcgtca tgatagtcac agcaaacccc agtaaaacac
     1201 cgtacactaa accccagatc tttagtccaa tataggggat accgaatagt aatatcgcta
     1261 taggaagcat ggctatattt cttaacacat ggtgcaagaa taacttcgga tttatcaaga
     1321 aagcgtccag caatgatttt gcaccaggaa agtcattttg cattacgtag ttataatcaa
     1381 gatagggatt aagttgaaag cggccggatt ctacctcatg gagcgaataa ttctgcctaa
     1441 aagcgagcca cgtccttttg gtgcccgggt ttaagtcgtc aacgttgagt ataacgtaga
     1501 cgatcagcat ggtgatgatc aaagcgacaa aaaccggcaa agtcatacga atcttttgga
     1561 gcacaaattc gaatcgcttg ttcttgcggt tcgagacagc catgtacgtc agaaaagcgg
     1621 caaaggcgag cgtggggaat acgtactcca atcttgtcag cgaggataaa caaagtagca
     1681 gcaaagctat accctgatgc ttgttcagga aaaataacgc gagtaaaaac aaccccaacg
     1741 cgacatgata aacgagaact ctttcgaaaa tattgactcc ggatgcgacc caaataaggg
     1801 tcagcagtaa tgccagcgaa cgcgatctaa gatgttctac taaggtcaaa agaagcagtg
     1861 tggaaacggt gatctttacc aagtactgca tgtaaaacac actttgaatc gagccgaata
     1921 tttttattaa cgatgagtaa agcaggttgt aaaccggcga gatgaatata gctccaagcg
     1981 tatcgccatg aagaaaccgg gccccttggc cgacgtaaat ggcctcatcc catatgggta
     2041 agtccatcgt attatgcaat ggataaagga gcagaaactg aactaccatg acacatacgt
     2101 acggcaacag ccgagctagc atctttaaat cctccagaat tgttacaggt attagcaata
     2161 cactttggag aggtcattcg gccggatccg ttagaatacc catcaacggt tgatttcgcg
     2221 ttcactttca agcgccatat gtgcttaatg cgctaacgat tctcgacggt gggcatccgg
     2281 atcatgcaag cgcgcaagca gactcagccg gactttcatg cttttctgtc atcacgcgct
     2341 tttgcaagag aaaccaatga gaatttttgg tggagccgaa gggaatcgaa ccctcgacct
     2401 cttgaatgcc attcaagcgc tctcccaact gagctacggc cccacacgaa gaatacggaa
     2461 atcggaaggc tgaactgaac tgtgagctaa tatctaaatt cagtgggcaa aagtcaatgt
     2521 ccggcggcgc atccggaaaa cgttttgaac gattttaact gctttaacgt tttgaaccaa
     2581 tcaagtcagt cccgtccgcg gaccatttcc tcggcgtgtt tgcgggtctg ttcagtgatc
     2641 tttatgccgc ccaacatgcg cgcaacttcg gcaatgcggt ctttctccga gagtttgtgc
     2701 acccgggtga aggtgcgtcc cttttcgact tccttttgca ccacgtaatg gcaatcggcc
     2761 agggccgcga tctgcggcag gtgggttacg ctaatgacct ggttctgggc cgcgacctgc
     2821 ttcaattttt tgccgacgat ctcggcgacc cgcccgccga cgccgctgtc gacttcgtca
     2881 aacagcaaag tcgacactac accgggcgtc atgaccaggg acttgagcgc cagcatgagg
     2941 cgcgacagct cgccgccgga ggcgatcttg gccaatggtt tgaccggctc gccgggattg
     3001 gatgaaaagt aaaattccac gtggtccata ccgcgctcgg tcaaccgctt gccagcgatg
     3061 aagaacggcg gcacgtcggc tttctcgtcc tggctcagaa atcgagcttc gaaagcgacc
     3121 tccgccatgc ccaaaccgcg cgcctccttc tccaactcgc ggcgcagttt tttcgccgaa
     3181 cgccggcgtt cattggatag tttctcagcg ctattccacg cgctcccgcg tgccgccgca
     3241 aaagtctttt ccatggcagc gatttgttcc tcgctatttt ccaaacgctc gagcgattgt
     3301 tggatgtccc cttccattct caggatgtca tcaatactgg cgttatactt gcgcttgagc
     3361 cgctgaatct ccgccaagcg gtcttcaagc tgttccaagg cccgcggatc aaagtgcacc
     3421 cgttcagcat agcgacgtag ctgggccgcc gcctcttgca attgtgccag agaagactgg
     3481 atcagctctt cggttggttg taaattggca tcgatgccgg ctagctcacg cagctttgcc
     3541 gcatacttgc cgagccggcc gaccaatgca gcgtcgcctt cgtacagcaa gtcttcaccc
     3601 tcgcgacagc ccagcgtgag tttttccgag tgcgccaaaa tattcttgcg cgcgcgcagc
     3661 tcttcctctt cgccggtttt gagttgggcg cgggcaatct cttcgacttg cgatgccaaa
     3721 ttagcccgtt cgcgccggcg ccggtccaaa gagtcacgcg tttcgttgag atcactccaa
     3781 gccgccgtca gagcgacaaa ctgctgtttc atcgctaaag tcatggcgtc gacatcggcg
     3841 aaggcgtcga gtaaatttaa gtgggtttca ggctgcagca agctgtgatg ttcatgctgg
     3901 ccgtaaatat gcaccaggga gccgccgata tccgcgagaa cattgagtga acaaagactg
     3961 ccgtttagat aaatgcggtt cttccccgat cgattgagga tacgcttgac taccagttca
     4021 ccatcgagtt cgaagccggc atcggcgagc tttctctcga tgggcttggg cacgccgtcg
     4081 aacaaagcct cgacggtcgc ctcttcttcc ttgtggcgaa tgatttccga gctgccccgt
     4141 tcgcccgaga caagtcccaa ggcatttaga ataattgact tgcccgcacc ggtttcgccg
     4201 gtgattatgt tcaatcccgg cccaagctca agtgccactt cgtcgatcac cgcaaagttt
     4261 ttgatccgca attcgcgtaa catatcctgg tcgaactagc gctctcccca tttgagcttt
     4321 tcacgcagaa tttcatagta gctacgcgac ggcagcttga taagcgatac cggtaaaccg
     4381 aaatcgcgaa cttccacctt gtcgccgttg ttgagacgca gccctttttg tccatccggg
     4441 ctcagaatca ccgtgtcacc aaaggatcgc agggtcacat gaattggcgc gtcgctcggc
     4501 acgacgatgg gccggttagt cagcgtatgg gggcagatcg gcgataacac aatcgcgccc
     4561 aaggtcggtt cgaggatcgg tcccgccgcc gccagtgaat acgccgtcga tcccgtaggc
     4621 gtggagataa tcaagccgtc ggcgcggtaa gtgcaaagat agtcatcgcc gatgtaggtt
     4681 tccaagtcga tgatgcggct gcgcgcgccc ttggtgatca ctgcgtcgtt gagtacgcgg
     4741 aagcgccgta ggcggccctt cttgctgatc acccgcactt ccaaagtcat gcgcttttcc
     4801 actccataat cgccggcgag ggttttgccg agcaccgatc tcaattcgtc caccgccact
     4861 tcggtgatga agcccaggcc gcccatattg acgcccaaaa tcggcacctc cggccgttct
     4921 accagcggag cgatattgag cagcgtgcca tcgccgccca gggagatgat caaatcggcc
     4981 tgcgtcgcca tttccttctt ggtacaagac tgcgcgccgc gcggcgtcaa accattttca
     5041 acgcagacct ttttgccgcg tttcctgagc caggggatca gccagcggac cgtctcggcg
     5101 gccttgggtt catggtattt ggcaactatt ccaacggtgc gaatcatgcg ggtagcgtct
     5161 ccagcggtga gagtatcaca tggccaaaaa aaaagtccat gacggcagaa actccgaaaa
     5221 ccgaatttgg catccgaaat aaactcaccg tcaaatacgt ctcaagtcag gaaatattca
     5281 accctcgaat ccgattggag tttgttttgg ttttgacgaa tttatcattt gaatatgttt
     5341 cgagcttcga gtcccggtcc gcgcggcttc ttttcgtgcg gcaaattttt gccttattgc
     5401 agtcgtaatt ttagacgacc aggcggcgca aacgcgaact gcagtaatct acgagcatga
     5461 ccagggcgat aagtactaac acgatggccg ccgcttcttg atactggaag gtgcgaaagc
     5521 tggtgttcat cagatagccg ataccgccgg caccgattag gcctaacacc gaagcggcgc
     5581 ggatgttggt ctccaagcgg tacagagtga atgagatcag atcgggcagg atctgcggca
     5641 ccacggcgaa agaaagaatc tgtgaataac cggcgccggt agcgcgaatc gcttcgacta
     5701 ctccctcgtc gatgttctcc accgactcgg acaacagttt acccaacacc gtcgcggtat
     5761 gaatggccaa tgcgatggtg ccggcgaaag cgcccagccc gacggcggcg acaaaaagca
     5821 gccccagggc gagatcgggt atcgtccgca ccaaatttag caacaggcgc acgccgcgat
     5881 agaccgccgg atgtggagtg gtattgcgcg cggctaagaa gcctaatggg aaggcaacca
     5941 gcagcgccag gaaggtgcca gctaacgcca tctggatagt ttccagggtc gcccccatca
     6001 cggtctgggg aaacgtattg tcccaccaac gttgtcgggc cctggcgtcg gcctcggcca
     6061 aaggcgccgg cagcaacagc tcacctaacg acaactgctc cggaaaatag tagtgcttcg
     6121 cgtcggtgat cttagaaaaa tccggcggca gcatgcgcgc cagagtctgg ccaatctgcg
     6181 gcacgccttc gatcaactca ccaggtcgaa tcttcgcgcc atgaaacgac caaaccaaga
     6241 cggcgagcag aatccctaaa aaaaccaaaa accgcccact ccggtggggc ctgaccggcg
     6301 cttcgttcat gatatcgcag gctatttcct gattgcgatc cgcggccaat cgactactcc
     6361 ttacggcatt acgatctgca aaagttgatg cgacaggcgc gaatttttag ggcggcgaaa
     6421 tttttcgact tcggatattt ttctcgccaa ggcgcaaagt cgccaagttc ggattccttg
     6481 tcatttcgac ccttgggaga aatctctcgt agatccttcg catccctcgg gatgacggtt
     6541 atcgcccgtc acctttgcgc cttagcggga gatattccga gtttcgctcg cggctctgcc
     6601 gcgctaggct ctctgtggtg aatccttcgg ttcattcacg gtttcgcctt cgccaaatac
     6661 tctgcctcat tgtactcgtc ggtggcgaga ttcttcaggc cgatcttgtc gtagagttcg
     6721 ccgaggacaa aatgcagcgt aggctcgtcc gagtcggcgc tgatcgcctc ggtcaagata
     6781 tccctggcct catggaacag ttgtttcgac ataagcagat tcgccctgag caccgccaat
     6841 gtggtcttcg acgccctgcc catggcaccc agttcggcca aacgttcctt gaccgcctga
     6901 gcctccaacc cgccgagcag tttgaaagtc gttttgctgg cggcgacgcc ttttctctcg
     6961 attgaccagc cgtattcgac gtcggcctgc aagcgcggcg ctgtcaccgg ataggcgatc
     7021 ttggttagat tgatattgtc ggcgctccag atctctccct gctggccaaa gatcttaacc
     7081 gtgacaggct gctggtccgt gcccgtccag tggaagatcg ggctatcgga caatatcgcg
     7141 ctgttgcgcg gcgacaacag agtcggcggc tgcgctttgc cccgcacaga caacgcgaca
     7201 taggtcggtg gctttttctt gcccacaagc aagttggcga cttcctttac ccgagcaccg
     7261 gcggcgctat ccttggccga tgacgcccgg gcttcaaaaa ttggactctt ctcgtccacg
     7321 gtgaccacgc gcgagccatc ggtatagaaa ataaccgcta aggcgtcttt cgtcgtctgc
     7381 acgacgttgc cggtatagag cgattgcaac accgcaggct tcgttgccgc agcgccattg
     7441 gtcgcgcgca actgtatatc gccgcgattg aatttaagct cggtaatgac cgcgatcgca
     7501 tcctgggcag aggctgcccc gaccaacgac acgagaaaaa agaatgctgc cgaccaaatt
     7561 aaaaccagtg ccttttgcat ggcgatcaat caacctccca ccgctccagc ggctctgact
     7621 tcaaacactt ctacggcttg agcccggccc ttgacctgca ctgtaccaca ggggcggatg
     7681 acaaactgcc ctttgagttg ccccatggtt tcccgggtaa tcagaatcgt cgtgccgtac
     7741 tgtttgttta gaccttctac cctcgatccg gtattgacgg tatctccggt cacgtcatac
     7801 ttcttgcgct ctcccgtgcc aacgacgccg atcaccaccg gcccgctgtg aatcccgaca
     7861 ccgatgcgca gacgc
//
LOCUS       DASQKS010000501         5334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1950881, whole genome shotgun sequence.
ACCESSION   DASQKS010000501 DASQKS010000000
VERSION     DASQKS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950881"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VE170_12910"
     CDS             <1..370
                     /locus_tag="VE170_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYJ16386.1"
                     /translation="DDVRFSMRTDKHAVEPFGSSDGLPGGKGACRVNPATTQEKALPS
                     RFGDHRLERNDTIRVERPGGGGLGKPFNRPIEKVLEDLRQGYVSVARAQSDYGVAAQM
                     VDGEPTLHEGETEYLRGKVG"
     gene            444..752
                     /locus_tag="VE170_12915"
     CDS             444..752
                     /locus_tag="VE170_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HYJ16387.1"
                     /translation="MAAAKKKAPAKKKAAAKKKRKPNAAFMKPVQPDTKLALVVGSKP
                     IPRTEVTKKLWQYIKKNGLQDQKKRTNINADDALKDVFGGKKTVTMFEMTKLVSKHVK
                     "
     gene            complement(938..1966)
                     /locus_tag="VE170_12920"
     CDS             complement(938..1966)
                     /locus_tag="VE170_12920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16388.1"
                     /translation="MKKLLLLVLFSLTAISSVSAQTPFYQGKTVQIRVGFAAGGAFDV
                     WARVIANHLGRFVPGNPTFVVQNMTGGGSMVAANYIYGVAKPDGLTLGVVSPAMYIEQ
                     VTGKKEVQYDWFKFSFIGSPEKTDRIFYMRADLGIKTLEDMRTVAEAPRCGSTGIGSA
                     AYYFPRLLQDAFGLKLMIVPGYPGAADVNLAIEKGEVHCFSGTVQAYFGSEPLRTWSK
                     TGFARVLVQGGAKRDPRMANVPTLWELMDKNKTNEIYRRLAKILLIPDEMGRPFFGPP
                     GMPADRLKLLRDGFNKMMGDPELIAEAKKKGLEAAPLTGEELEALIKEAGVPSADIIQ
                     RLKPLMEN"
     gene            2267..2509
                     /locus_tag="VE170_12925"
     CDS             2267..2509
                     /locus_tag="VE170_12925"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYJ16389.1"
                     /translation="MRTATAKELRNKTSLILDEVRKGQSVTITYRSKPVAVLTPIDAQ
                     QPKALKTAGFGIWKDRKDNRNVEQWLNKLRQPRFTR"
     gene            2470..2889
                     /locus_tag="VE170_12930"
     CDS             2470..2889
                     /locus_tag="VE170_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYJ16390.1"
                     /translation="MAEQATTTSLYPVVFDTDILIWYLRGSEKALRAINHVAYSRRTI
                     SILSIMELTQGCRNGAEIRAVKLFVSENFTQVLYLKETVCRLASDLLERHALPNGLRV
                     VDALIAASTLNTNGSLATANLKHYRPIANLHLIPFKP"
     gene            complement(2957..4849)
                     /locus_tag="VE170_12935"
     CDS             complement(2957..4849)
                     /locus_tag="VE170_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ16391.1"
                     /translation="MPNMSELFPQRRVTPLPGGYMGKILRVDMTTGALTDENLPEEPD
                     LRRFIGGQALALYILMREQSLDVKPYDPAAKMVMMTGPLTGTGLTPGGTKVCAVFLSP
                     MTNNTLGRGAASGYWAAYLKQSGYDGIILQGAAKSPQYLFINDGKPELRDASKFWGKG
                     SRDTEESLRAAVGIKDARVMGIGPAGEHRVNAAMLCNDFNHSASHSGGAIFGSKMLKA
                     IVVHGTKRPPLRDKAALVEAGLRWRKTLQQYALKDRYSTGHARHLEALPNNNMQSTLI
                     GDHNRGFEQNRVVQRPCFQCARLCPWDVEIGEGEFKGQVGHFNAGAEWLDTFWNLGVK
                     GNATLYLAERINDLGIECGHFSFGAGVLFEAYQKGLVTEKDCDGLRLEWGNVEAIDKL
                     LEMTARRQGKWGNLIADGPLEVAEAIGGDAVNWVVHTKRGVPAMHDWRPHFANMLREI
                     VASGGMKPQGGGQANPPPDLKYREKWGPLDRDDPTGWPQSNVNAEQYRQFVGIMGGCW
                     FAQMHGKPDGLKSIVDSFNATTGWNFDLDEAMLAGHRSAILQSIFGTQRGWIADHDWQ
                     QVGQRFLDPVPDGKYQGFTIAKWLPEMVREYYQLSGRHEKTGRPYMDTLKLLGLEEFS
                     EWAQLD"
     gene            complement(4962..>5334)
                     /locus_tag="VE170_12940"
     CDS             complement(4962..>5334)
                     /locus_tag="VE170_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16392.1"
                     /translation="PGVKPLFDDTEEIIRYVKRTQIYPIIHIMAIREETAEKYPDLPA
                     KAIEAFREANRLAGNYFKPGEAEGYAKERDLLGYDPYAGVLTEADKKSMQALNRYQIE
                     QGLVKKELPLQSLFVKEAFRM"
BASE COUNT         1191 a         1601 c         1395 g         1147 t
ORIGIN      
        1 cgacgacgtg cgcttttcca tgcgcaccga caagcacgcc gtcgagccct tcggcagcag
       61 tgacggcctg cccggcggca aaggcgcttg ccgggtcaat cctgcaacca cacaagaaaa
      121 ggcgctgcca tcgcgcttcg gcgaccatcg gttagagcgc aacgatacca ttcgtgtcga
      181 acgccccggc ggcggcggcc tgggaaagcc gttcaatcga cccatcgaaa aagtgctcga
      241 agacctgcgc cagggctatg tctccgtcgc ccgcgcccag tccgattacg gcgtcgcagc
      301 tcaaatggtc gatggcgagc ccactctcca cgagggagaa actgagtatt tacgaggaaa
      361 agtcggataa ttcgatagca aaaatccggt acctctgcta tatttttatg aaataactta
      421 tttccttaag gaggaaaaca catatggcag cagcgaaaaa gaaagcgccg gctaaaaaga
      481 aagccgcagc gaagaagaaa agaaaaccca atgcggcgtt catgaagccg gtacagcccg
      541 acaccaaact cgccttggtc gtcggcagca agccgatccc gcgcacagaa gtgaccaaga
      601 aactgtggca gtacatcaag aagaacggcc tgcaggatca aaagaaacgc accaacatca
      661 acgccgacga cgcgttgaaa gatgtgttcg gcggcaagaa aaccgtcact atgtttgaaa
      721 tgaccaaact ggtcagcaaa cacgtcaagt aattttactt accgaataat gccaaaggcc
      781 gagaacatcg ccgagccggt gtccttggcc tttgtgtttg catctcacgt ttgtccgttc
      841 accctatatt tatggcgcat cgcggcgggt cctatctcgc tgcgattttc cctggcgccc
      901 gagtcacctc tgcagttttc tcgcatacgc cggagattta gttttccata agcggcttca
      961 gccgttgaat gatatcggcg gacggcacgc cggcttcctt gatgagcgct tcgagttcct
     1021 cgccggtcaa cggcgcggcc tcgagacctt ttttcttcgc ctcggcgatt agttccggat
     1081 cgcccatcat cttgttgaag ccatcgcgca aaagtttcaa acggtcggcc ggcatgcccg
     1141 gcgggccgaa aaacggccgg cccatctcat cggggatcag cagtattttc gccaggcggc
     1201 ggtaaatctc gttggtctta ttcttgtcca tcaactccca caaggtcggc acgttggcca
     1261 tgcgcggatc gcgcttagcg ccgccctgca ccagcaccct ggcgaatccg gtctttgacc
     1321 aggtgcgcaa cggctcgctg ccgaagtacg cctgcaccgt gccgctaaag caatgcacct
     1381 cgcccttttc aatggcgaga ttgacatctg cagctccggg ataacccggc acgatcatca
     1441 gcttgagccc aaaggcatct tgcaacagcc gtgggaaata gtatgcggcg ctgccgatcc
     1501 cggtggagcc gcaccgcggc gcttccgcca ccgtgcgcat gtcctccaag gttttgattc
     1561 ccaaatcggc gcgcatgtaa aaaatccggt cggtcttctc cggcgagccg atgaaactaa
     1621 acttgaacca atcgtactgg acttctttct tgccggtcac ctgctcgata tacatcgccg
     1681 ggctgacgac accgagcgtc agtccatccg gcttggccac gccataaata taattcgccg
     1741 cgaccatcga cccaccgccg gtcatgttct gcacgacaaa cgtcggattg cccgggacga
     1801 accgtcccag atggttggca atcacccgcg cccaaacatc gaacgctccg ccggcggcga
     1861 agccgacacg gatttgcacg gtcttgccct ggtagaaggg cgtctgggcg gagaccgaag
     1921 aaatcgcagt caagctaaag agtacaagca gcaaaagttt tttcacgaaa atgtcctctg
     1981 aggtttatta actaagccaa tcgattctta ctatcgtcat gcccgaatct tcaattcact
     2041 tgttaaaaga ctttcaatat catcttacgg aatcaacgag ccccttgggc gcagtcgagc
     2101 aatggccatc gtctgccggc aacacaccgc cattcctacc acaaaggaga agtgccggcc
     2161 cacctgttgc cgatcgtgaa gcgatccgca gatttcatac ccattccttg tagcggcact
     2221 cacctaagta tatactcttg agtgtatact tcattgggag actacagtga gaactgcaac
     2281 agcaaaagaa ttacgaaaca agacttcgct cattctcgac gaagtccgta agggccaatc
     2341 ggtgacgatc acttaccgca gcaaacccgt tgcggtgttg actcccattg atgcacagca
     2401 accaaaagcg ctgaaaaccg ccggctttgg catttggaaa gaccgcaaag acaaccgcaa
     2461 cgtggaacaa tggctgaaca agctacgaca acctcgcttt acccggtagt attcgatacc
     2521 gacatcttaa tctggtattt gcgtggcagc gaaaaagcgc ttcgggcaat caaccacgtc
     2581 gcctattcca gacgtacgat ctcgatcctc agcattatgg agttgaccca aggctgccgc
     2641 aacggcgcgg agatacgcgc ggttaaatta ttcgtatcgg aaaatttcac ccaagtctta
     2701 tacttgaagg aaacggtctg tcgactggcc agcgacctgc tcgaacggca cgcattgccg
     2761 aatggcttgc gagtcgtcga tgcactgatc gcggcctcga ctctcaatac gaacggttcc
     2821 ctggcgaccg ccaatcttaa acattatcgc ccgattgcca accttcatct aattccattc
     2881 aagccctagc cgcgaacgcg cgcaatgaca gattaatcaa gctacaggtt aaacttcatc
     2941 cgctcgacaa gtcgccttaa tccaactgcg cccattcgct gaactcttcc agccccaata
     3001 attttaatgt gtccatgtac ggccggccgg ttttttcgtg gcggcccgag agttggtaat
     3061 attcacggac catttcgggg agccatttgg cgatggtgaa gccttggtat ttgccgtcgg
     3121 gcacggggtc gaggaaacgt tggcccactt gctgccagtc gtgatcggcg atccagccgc
     3181 gctgggtgcc aaaaatgctt tgcaagatcg cgctgcggtg gccggccaac atcgcctcgt
     3241 cgagatcgaa attccatccc gtcgtcgcgt tgaatgaatc gacgatactc ttcaggccgt
     3301 ccggcttgcc atgcatctgg gcgaaccagc agccgcccat gatgccgacg aactggcggt
     3361 actgctccgc attgacgttg gactgcggcc agccggtggg atcgtcgcgg tcgaggggcc
     3421 cccacttctc gcgatacttc aaatccggag gcggattggc ttggccgccc ccctgcggct
     3481 tcatgccacc gctggcgacg atctcgcgca gcatgttggc gaaatgcggc cgccaatcgt
     3541 gcatcgccgg cacgccgcgc ttggtatgca ccacccaatt gaccgcgtcg ccgccgatcg
     3601 cttcggcgac ttcgaggggg ccgtcggcga tcaagttgcc ccacttgccc tggcgtcgcg
     3661 cggtcatttc taataattta tcaattgctt cgacattgcc ccactccaag cgcagcccgt
     3721 cgcagtcttt ctcggtgacc aaaccttttt gataagcttc gaacaatacg ccggcgccga
     3781 aagagaagtg gccgcactcg atgccgagat cgttgatgcg ctcggccaaa taaagcgtcg
     3841 cgttgccttt gacgcccaga ttccagaaag tatcgagcca ctcggcgccg gcgttgaaat
     3901 ggccgacctg gcctttgaac tcgccttcgc cgatttccac gtcccacgga cagagccgcg
     3961 cgcattgaaa gcaggggcgc tggacgacac gattttgttc gaagccgcgg ttgtgatcgc
     4021 cgatcagcgt gctctgcatg ttgttgttgg gcagcgcttc gagatgtcgc gcatggccgg
     4081 tactgtaacg atctttgagc gcgtactgct gcagcgtctt gcgccagcgc agaccggctt
     4141 cgaccagcgc cgctttatcg cgcagcggcg gccgcttggt gccgtggacg acgatggcct
     4201 tcaacatctt cgaaccgaag atcgcgccgc cgctgtgcga cgccgaatga ttgaaatcgt
     4261 tacacagcat cgccgcattg actcgatgct cgccggcggg accgatgccc atcaccctgg
     4321 cgtctttgat gccgaccgcg gcgcgcagcg attcttcggt atcccgcgat cccttgcccc
     4381 aaaatttgct tgcatcgcgc agctcaggct taccgtcgtt gatgaacaga tattgcggcg
     4441 acttcgccgc cccttgaaga ataatgccgt cgtatcccga ctgtttgaga taagcggccc
     4501 aataaccgct cgccgcgccg cgtccaagag tattgttggt cattggactt aagaacaccg
     4561 cgcaaacttt ggtaccgccg ggcgtcaagc cagtgccggt tagcggtccg gtcatcatca
     4621 ccatcttagc tgccggatcg taaggcttca catccagcga ttgttcacgc atgagaatat
     4681 acagcgccaa cgcttggccg ccgatgaacc ggcgcaggtc cggctcttcg ggtaaattct
     4741 cgtcggtcag cgcgccggtg gtcatatcga cgcgcagaat cttgcccatg tagccgccgg
     4801 gaagcggggt cacacgccgc tgaggaaata gctcagacat atttggcata acgtacctta
     4861 cgagaataaa tgaacaaatt ggagtactgg agtgatggag tattgggttt cggattcgcc
     4921 catcactcta ccactccatt actccaacac tccaattccg ctcacatcct gaacgcttcc
     4981 ttgacgaaca agctctgcag cggcagctct ttttttacca acccctgctc gatctggtag
     5041 cgattgagcg cctgcatcga tttcttgtcc gcctcggtca acacgccggc gtagggatca
     5101 tagcccaaca gatcgcgctc cttggcgtag ccttcggcct cgcccggttt gaaataattg
     5161 cccgctaggc gattggcttc gcgaaacgct tcgatcgctt ttgccggcaa gtcgggatac
     5221 ttttccgcgg tctcctcgcg tatcgccatg atatgaatga tcggatagat ctgtgtccgt
     5281 ttaacataac ggatgatttc ttcggtgtcg tcgaacaaag gcttgacgcc gggg
//
LOCUS       DASQKS010000502         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3655661, whole genome shotgun sequence.
ACCESSION   DASQKS010000502 DASQKS010000000
VERSION     DASQKS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655661"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VE170_12945"
     CDS             complement(<1..1466)
                     /locus_tag="VE170_12945"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYJ16393.1"
                     /translation="MSEGAIMEIRFRDSTADRIKSDLVVIPVRDKQLDAPTLRALDRQ
                     LKGQLRARMSKGDFAGAEGSVLLYPTAGMLPAPYLLLIGIGAGETTSDDTWRNAGARA
                     RKEACSIGAGEFAVFLAAEKNFDSAVGAIVEGAALAGYQFNKYRSNGKSVAALKSLTF
                     FRSGLKKTSALQTSVDIALTTAPGVFLARDLVNEPPSVATARFLGAQAQRVCQGRGLT
                     VEVWNKKKIEAMKLAGLLAVNRGSVEEPRFIRIRYRPAGKARKRVALIGKGITFDSGG
                     LSLKPSKSMETMKLDMAGGAAVIATMSCLPQLGVDIEVTGYVPTTDNLPGQNAQKPGD
                     VIRYMNGKTIEVLNTDAEGRLILADALALAAQQKPDYMINLATLTGACMTALGTQVGG
                     LFSNHQPLADSLLRSGREAGEKLWQLPLVKEYKELIKSSVADMKNIGGAHGGAITAAL
                     ILQEFVGDTPWAHLDIAGPAFAESDNLLGPKGGTGFGVR"
     gene            complement(1463..2038)
                     /gene="folE"
                     /locus_tag="VE170_12950"
     CDS             complement(1463..2038)
                     /gene="folE"
                     /locus_tag="VE170_12950"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HYJ16394.1"
                     /translation="MSPAYRGAIMEEYIRQILKAIGEDPEREGLIKTPQRVAEALTFL
                     TRGYDMDPEKVINDALFTEDYSEMIVQRDIDFYSLCEHHMLPFIGKAHVAYIPHHKIV
                     GISKLARLVDIYAARLQVQERMTNQIATTIMEKLDALGVAVVLEAEHLCMRMRGVEKQ
                     NSYVITSSLLGAFRTRQETRAEFMNLIRRKD"
     gene            complement(2102..2839)
                     /locus_tag="VE170_12955"
     CDS             complement(2102..2839)
                     /locus_tag="VE170_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HYJ16395.1"
                     /translation="MTDVNVFVAFAAGIFSFLSPCVLPLIPSYLSFVSGVSLDEMRGE
                     QARAQLHWRVVLNSLAFIFGFSLVFVSLGASASFLGSLFMGYRNEIRIAGGVFILIVG
                     LYLVGAFKIAALDQYLQFNLKDKPAGYLGSVLVGITFAVAWTPCVGPILGATLALAST
                     SGEIGRGTYLLMSYAAGLGLPFLLSALAINSFFQFSQKLRRYIQAIHVGGGILLIVVG
                     ILLITDYMTYLNAYALRFTPTWLLQRL"
     gene            complement(2836..>3102)
                     /locus_tag="VE170_12960"
     CDS             complement(2836..>3102)
                     /locus_tag="VE170_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ16396.1"
                     /translation="KDKNFVILAVAVKDRKQDAIDFVKELKLTYPIALDPDAKVGQEY
                     GAWGLPATYIIGPKGEGLARGWGPADWHSAGARKLIKDLTDGKR"
BASE COUNT          676 a          860 c          927 g          639 t
ORIGIN      
        1 cgcacgccga agccggtgcc gcctttggga ccaaggagat tgtcgctttc ggcaaaggcc
       61 gggccggcga tgtcgagatg ggcccatggg gtgtcgccga cgaactcttg caggatcagc
      121 gccgcggtga tggcgccgcc atgggcgccg ccgatgtttt tcatgtcggc gacactgctc
      181 ttgatcagct ctttatattc cttcaccagc ggtagttgcc acagtttttc accagcttcc
      241 cggccgctcc gcagcagact atccgccaac ggctggtggt tgctgaagag gccgccaact
      301 tgggtgccga gagcggtcat gcaggcgccg gtgagcgtgg ccagatttat catgtagtcg
      361 ggcttttgct gcgccgccag ggcaagcgcg tcggcgagaa tcaagcggcc ctcggcgtcg
      421 gtgtttagca cttcgatggt cttaccgttc atataacgaa tcacatcgcc gggtttctgt
      481 gcattctggc ccggcagatt atcggtggtc ggcacatagc ccgtgacctc gatatcaacg
      541 cccaactggg gcaggcaact catggtggcg atcaccgcgg cgccgccggc catgtcgagc
      601 ttcatggtct ccatcgattt tgacggtttg agtgatagac cgccggaatc gaaagttatg
      661 cctttgccga tcagggcgac gcgcttgcgg gctttgcccg cgggccggta gcggatacga
      721 atgaagcgcg gctcttccac gctgccgcga ttaaccgcca gcaggccggc caacttcatc
      781 gcttcgatct ttttcttgtt ccaaacttcg accgttaggc cacggccttg gcagacgcgc
      841 tgggcttgcg cgccgaggaa cctggccgtc gccacagacg gcggctcgtt caccagatcc
      901 cgcgctagga agactccggg ggccgtggtg agcgcgatat ctaccgatgt ttgcaacgcg
      961 ctggtttttt tgagaccgga gcggaaaaat gtcagagact tcaatgccgc gacggacttg
     1021 ccattcgagc ggtatttgtt gaactgataa ccggccagtg cggcaccctc gacgatggcg
     1081 ccgacggcgc tgtcgaaatt tttttccgcg gccaagaaca cggcgaattc gccagcaccg
     1141 atggaacagg cttctttgcg cgctctggcg ccggcattgc gccatgtgtc atcgctagtg
     1201 gtttcaccag cgccaatacc gattagcaga agatagggtg ccggaagcat gccggcggtc
     1261 ggatacagca aaacactgcc ttcggcgccg gcaaaatcac ctttgctcat gcgcgcgcgc
     1321 agttggcctt tgagctggcg atcgagggcg cgcagcgtcg gggcgtcgag ttgcttgtcg
     1381 cgcacgggga taacgaccag gtcggacttt atgcggtcgg ctgtactatc gcgaaagcgg
     1441 atctccatga tggcgccctc gctcaatcct tgcgccggat cagattcatg aactcagcgc
     1501 gggtttcctg gcgggtacga aaggccccga gcaagctgct ggtgataaca taggagttct
     1561 gcttctcgac gccgcgcatg cgcatgcaca gatgttcggc ttcgagcacg acggcgacgc
     1621 ctagtgcgtc gagtttttcc atgatcgtcg tggcgatctg gttggtcatg cgctcctgga
     1681 cctgcaaacg agccgcgtaa atgtcgacca gccgggcaag tttggaaata ccgacaattt
     1741 tatgatgggg aatgtaggcg acatgagctt tgccgatgaa cggcaacatg tggtgttcgc
     1801 acaacgagta aaaatcgatg tcgcgctgga cgatcatttc agagtagtct tcggtaaaca
     1861 aagcatcgtt gatcaccttc tcggggtcca tgtcataacc gcgggtcaaa aaagtcagcg
     1921 cctcggcgac gcgctgcggc gttttaatca gtccttcacg ctcgggatct tcgccgatgg
     1981 ctttgagaat ttgccgaata tattcttcca tgattgctcc gcgataggcg ggactcattg
     2041 agctagagta aagtctcaaa aagctaacat ttagccggtg acgggtcaaa caaactagct
     2101 gctagagccg ttgcagcagc caagtgggcg tgaaacggag cgcgtaggcg ttcaaatagg
     2161 tcatgtaatc ggtgatcaat aggatgccga cgacgatcaa gagaatgccg ccgcccacat
     2221 ggatcgcttg aatgtagcgc cgcaatttct gcgagaactg gaaaaatgag ttgatcgcca
     2281 acgcgctcaa aagaaatggc agccccagac ccgcggcgta actcatgagc aagtaggtgc
     2341 cgcggccgat ttcgccggaa gtgctggcca atgccagcgt cgcacccagg atgggaccga
     2401 cgcaaggagt ccaggcgacg gcgaaggtga taccgacgag gaccgaacct aaataaccag
     2461 ccggcttatc tttcaggttg aactgcaagt attggtcgag ggcggcgatt ttgaaggcgc
     2521 cgacgagata gagtccgacg atcaggataa atacgccgcc agcgatgcga atctcattgc
     2581 gatagcccat gaaaaggctg ccgagaaaac ttgcagaggc gccgagggaa acgaagacca
     2641 aggagaagcc gaagataaac gccagcgagt tgagcacgac gcgccagtgt agctgcgcgc
     2701 gcgcctgctc accgcgcatt tcgtcgaggg aaactcccga aacaaacgaa agatacgatg
     2761 gaatcagcgg caatacgcag ggagagagaa aagaaaaaat cccggcagcg aaagcgacaa
     2821 agacgttgac gtcggtcatc gtttgccgtc ggtcaagtct ttgatcagtt tacgcgcgcc
     2881 cgcgctatgc cagtccgccg gtccccagcc ccgcgccaaa ccttcgccct tgggacctat
     2941 aatataggtc gctggcaatc cccaggcgcc gtattcctga cccactttag cgtcgggatc
     3001 tagagcgatc ggataggtca gcttgagttc cttgacgaaa tcgatcgcgt cttgtttgcg
     3061 atctttcacg gccacggcaa gaatcacaaa gttcttgtct tt
//
LOCUS       DASQKS010000503         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3655808, whole genome shotgun sequence.
ACCESSION   DASQKS010000503 DASQKS010000000
VERSION     DASQKS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655808"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="gnd"
                     /locus_tag="VE170_12965"
     CDS             <1..793
                     /gene="gnd"
                     /locus_tag="VE170_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYJ16397.1"
                     /translation="IQRVVDKGAVGAHSRADFVKQLSLPRAIWLMVPSGDVVDQTIEQ
                     LLPSLAAGDIIIDGGNSNYKDSIRRAETLRRHNLHFVDAGTSGGIWGLQNGYCLMVGG
                     EPEIVSRLEPIFLTLAPKDGYLHAGPNGAGHFVKMIHNGIEYGMMQAYGEGFELLKAS
                     QFDLDLAKISHLWNQGSVVRSWLLELCESAFEKDPKLDAIKGYVEDSGEGRWTVIEAI
                     EKGVAAPTLAHSLFARFASRQQDAFSNKVVAALRNEFGGHAVKKG"
     gene            799..2343
                     /gene="zwf"
                     /locus_tag="VE170_12970"
     CDS             799..2343
                     /gene="zwf"
                     /locus_tag="VE170_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYJ16398.1"
                     /translation="MASTPHEYVATGLSQAKRPHACVLTIFGASGDLTKRKLIPALYN
                     LALEKRLPQRFAVVGYARSEMTDEEFRDKMRAAVKEFSRTGLQDEQVWQEFAKTLYYV
                     RGDYDNESGYERLKQFIENFDRGSRVLPARVFYLAIPPEQYGGVIERIAAAGLAPKDY
                     GAEPHARVVIEKPFGTDLQTARELNQRVHEALGENQIYRIDHYLGKETVQNIMVFRFA
                     NAVFEPIWDRRYIDHVQITAAESVGVETRGGYYESAGVVRDMFQNHLLQLLCLTAMEP
                     PVGFNADAVRDEKGKLMRAVRPIAPEEVSTVAVRGQYGAGTVDGSPVVAYRDEPGVAK
                     DSTTVTYAAIKLAIDNWRWEGVPFYLRSGKRMAKRVTEIAIQFKRPPMLLFKSSAVED
                     VSPNVLVMRIQPDEGISLRFDVKPPGPDLTVSPLSLKFNYSEAFGNSSPEAYETLLED
                     CIEGDSTLFTRHDWVELAWALMDPIIQVWSLSKPRNFPNYPAGSWGPKEADDFMQRDG
                     RRWRKP"
     gene            2654..3655
                     /gene="glk"
                     /locus_tag="VE170_12975"
     CDS             2654..3655
                     /gene="glk"
                     /locus_tag="VE170_12975"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HYJ16399.1"
                     /translation="MALRNLVLAGDIGGTKTNLSVFTVRGDKLHSESLRTFPSKRYSG
                     LVPVLREFLAGNAAKFDAACFGIAGPVVDGTVKTPNLPWLIDTAELRRALNLDNVTLL
                     NDLEAGAYGIFTLDDDAFCTLNQGTARQSGSKALIAAGTGLGQAILYHDGEHFRPLAS
                     EGGHADFAPRNELEIELLRHLIERFKHVSYERALSGPGLFNIYGFFKERIGLEEPQWL
                     TERFAASDDLSAVVSKAALAGEADICIKSLDLFVSIYGAEAGNLALRAKSVRGLYIGG
                     GIAPKILSKLTDGTFMRSFVDKGRYTDLVAAIPVQVVLNDLAALRGAGYYAAFLARD"
     gene            3648..4388
                     /gene="pgl"
                     /locus_tag="VE170_12980"
     CDS             3648..4388
                     /gene="pgl"
                     /locus_tag="VE170_12980"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HYJ16400.1"
                     /translation="MTDREVIICRDVDELSRKAAERFVGLARQSIAARGRFAVALSGG
                     STPKALYSLLATAEFSEQLDWRQIHLFFGDERCVAPDHAESNYRMVAESLLTKIAIPD
                     KNVHPMAGELAPEAAAAAYAAELTGFFDLKEDQPPRFDLIFLGLGEDGHTASLFPDSS
                     ALHETEALVAPVYVEKLKAHRLTLTLPTINSAGQISFLIAGQSKSAVVKSIFTGQNTE
                     APAARIKPGHGKLTWFITEDAAGDLGKA"
     gene            complement(4422..5000)
                     /locus_tag="VE170_12985"
     CDS             complement(4422..5000)
                     /locus_tag="VE170_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4779..4781),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HYJ16401.1"
                     /translation="MAGLIAGVLTANPLFAQLGPKDPTSGSATELDRIKIGQAAPDFG
                     LENVEGQVIKLSDFRGKKAVVLVFYRGYWUPFCMEQLGKLKSLLTKAQKDKVQILAVS
                     VDTRDESKKFGETLRKRFDGEFDFPLLEDKDHKVIDRYGIFNPDGKGWPYPSTFVIDP
                     QGVVRWRFIEKTQDKRPSNQQIVAELRKIKSL"
     gene            complement(5079..>5301)
                     /locus_tag="VE170_12990"
     CDS             complement(5079..>5301)
                     /locus_tag="VE170_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16402.1"
                     /translation="HNSEPQSPPALFVGSGVVSEAVMQVIERDRFERIVPRWLALMCW
                     LQRLSPALFRYLAQRRFRPYVTLPANRRS"
BASE COUNT         1105 a         1544 c         1568 g         1084 t
ORIGIN      
        1 aattcagcgg gtcgtcgaca agggtgccgt gggcgcccat tcgcgcgcgg atttcgtcaa
       61 gcaattatcg ctgccgcggg cgatttggct gatggtgccg tcgggcgatg tggtggacca
      121 gaccatcgag cagttgctgc cgagtctcgc tgccggcgac atcatcatcg acggcggcaa
      181 ctcgaattac aaggattcga tccggcgcgc cgagacgctc aggcggcaca acctccactt
      241 cgtcgacgcc ggcaccagcg gcggcatctg gggtctgcaa aacggctact gcctgatggt
      301 cggcggcgag ccggaaatcg tcagccgcct ggaaccgatc tttctcaccc tcgcgcctaa
      361 agacggctat ctgcacgccg ggcccaacgg cgccggccac ttcgtcaaaa tgattcataa
      421 cggcatcgag tacggcatga tgcaggcgta tggcgaaggc ttcgagctgc tcaaagcttc
      481 gcagttcgat ctcgacctag cgaagatttc ccatctctgg aatcagggca gcgtggtgcg
      541 ttcctggctg ctcgagttat gcgaaagcgc cttcgaaaaa gatcccaagc tcgacgccat
      601 caaaggttat gtcgaggatt cgggcgaagg gcgctggacg gtcatcgaag cgattgaaaa
      661 aggcgttgcg gcgccgactt tggcccacag tttgttcgcg cgcttcgcgt cgcgccagca
      721 ggacgccttt tccaataaag tcgtcgccgc gctgcgcaac gagttcggcg gtcatgcggt
      781 gaaaaaaggc tagctgacat ggcgagcact ccgcatgaat atgtcgccac gggtttgagc
      841 caggcgaaaa ggccgcacgc ctgcgtgttg acgatctttg gcgcatccgg cgatttgact
      901 aaacgcaagt tgattccggc gctgtacaat ttggccttgg aaaagcgctt gccccagcgt
      961 ttcgccgtgg ttggctacgc gcgcagcgag atgaccgacg aagagtttcg cgacaagatg
     1021 cgcgcggcgg tcaaagagtt ctcccgcacc ggtttgcaag acgagcaggt ttggcaggag
     1081 tttgccaaga cgctgtacta cgtgcgcggc gactatgaca acgaaagcgg ctacgagcgc
     1141 ttgaagcagt ttatcgaaaa cttcgatcgc ggcagccggg tattgccggc gcgggttttt
     1201 tatctggcga tcccgccgga gcagtacggc ggcgtgatcg agcgcatcgc cgccgccggt
     1261 ttggcgccga aagattacgg cgccgagccc cacgcccggg tggtcatcga aaaacccttc
     1321 ggcaccgacc tgcaaaccgc gcgggagctc aaccagcgcg tgcatgaggc gctcggcgaa
     1381 aatcaaattt accgcatcga ccactatctc ggcaaagaaa cggtgcaaaa catcatggtg
     1441 tttcgcttcg ccaacgccgt gttcgagccg atctgggacc gccgctacat cgatcatgtg
     1501 cagatcaccg cggcggaaag cgtcggcgtc gagacccgcg gcggctacta cgaatctgcc
     1561 ggcgtggtgc gcgacatgtt tcagaatcac ttgctccagc tgctctgtct gacggcgatg
     1621 gagccgccgg tgggatttaa cgccgatgcg gtgcgcgacg agaagggcaa gctcatgcgc
     1681 gcggtgcggc cgatcgcgcc ggaagaagtc tccaccgtcg ccgtgcgcgg ccaatacggc
     1741 gccggcacag ttgacggcag cccagtcgtt gcgtatcgcg atgagcctgg cgtcgccaag
     1801 gattccacca ccgtgactta cgcggcgatc aaactcgcca tcgacaactg gcgctgggaa
     1861 ggcgtgccgt tttatctgcg ctcgggcaag cgcatggcca agcgggtgac cgagatcgcc
     1921 attcaattca agcggccgcc gatgctgctc ttcaaatctt ccgccgtgga agatgtcagc
     1981 cccaacgttt tagttatgcg cattcagccc gacgaaggca tctcgctgcg cttcgatgtc
     2041 aaaccgccgg gaccggacct caccgtcagc ccgctgagtt tgaaattcaa ttactcggaa
     2101 gcctttggca acagttcgcc ggaggcctac gaaaccttgt tggaagactg catcgaaggc
     2161 gattcgacat tattcacccg ccacgattgg gttgaattgg cctgggcgtt aatggatccg
     2221 atcattcaag tctggagttt gagcaagccg agaaactttc ccaattatcc agcgggatca
     2281 tggggcccga aagaagccga tgatttcatg cagcgcgatg gccggcgctg gcgtaaaccg
     2341 taaggggaga gggattgccc cacacccttc gaattgctca gggaatcgga aaagggcaaa
     2401 gggcacaaaa cgtaaaattc acaattgtcg gtcgttgtaa ggttgcgcgc ttcatgtcct
     2461 ccttagaaaa ccatgttagg gagcgtctta cccgcgaaag cgggtatcca ggccaatttg
     2521 atgcagacca aacctggatt cccgcgtgcg cgggaatgac ggatcaagca gaccgcttaa
     2581 aagagggaat tccatccatt tttattatcg gcagacgcgt tagctcacag acacttcgtg
     2641 gtgaatcata atcatggcgt taagaaatct cgttctcgcc ggcgatatcg gcggcaccaa
     2701 aaccaacctg tcggtgttca cggtgcgcgg cgacaaactg cacagcgaat cgctgcgcac
     2761 gtttccgagc aaacgctatt caggcttggt gccggtgctg cgagagtttc tcgccggcaa
     2821 tgcagctaaa ttcgacgcgg cgtgtttcgg catcgccgga ccggtggtcg acggtaccgt
     2881 caaaactccc aatctcccct ggctgatcga taccgcggag ttgcgccggg cgctcaacct
     2941 cgacaacgtg accttgctca acgatcttga agccggcgcc tacggtattt tcaccctgga
     3001 cgatgatgcg ttctgtacgc tcaaccaggg caccgcgcgc cagtcaggga gcaaagcgtt
     3061 gattgccgcc ggcaccggtc tgggacaagc gatcctatac cacgacggcg agcattttcg
     3121 tccgctggcg tccgaaggcg gccacgccga ctttgcgccg cgcaatgagc ttgaaatcga
     3181 actgctgcgc catctgatcg agcgcttcaa gcatgtcagc tatgagcgcg cgctttccgg
     3241 ccccggcctg tttaacatct atggtttttt caaagagcgg atcggtctgg aagagccgca
     3301 atggctgacc gagcgttttg ccgcttcgga cgatctgagc gcggtggttt ccaaggcggc
     3361 gctggccggc gaggcggata tttgtatcaa gagtctggac ctcttcgttt cgatttacgg
     3421 cgccgaggca ggcaacctcg cgctgcgcgc caagtcggtg cgcggtctct acatcggcgg
     3481 cggcatcgcg ccgaagattc tcagcaagct tacagacggt accttcatgc gcagcttcgt
     3541 cgacaagggg cgctacaccg acctggtcgc cgcgattccc gtgcaagtgg ttttgaacga
     3601 tctggccgcc ctgcgcggcg ctggctatta tgccgctttc ctcgcccgtg actgaccgag
     3661 aagtcatcat ttgccgcgat gtcgacgaac tcagccgcaa ggcggcggag cggttcgtcg
     3721 gattggcgcg ccaatctatt gccgcgcgcg gccggttcgc cgtggccttg tccggcggct
     3781 cgacccccaa agcactgtat tccttgctag cgacagcgga attcagtgaa caactcgact
     3841 ggcggcaaat tcacctattc ttcggtgacg agcgctgcgt ggcgccggat catgccgaga
     3901 gcaattatcg aatggtcgcg gaatcgttgc tgaccaagat tgccattccc gataaaaatg
     3961 tgcaccccat ggccggtgaa ctcgcgcccg aggcggcggc cgcggcgtat gccgcggagt
     4021 taaccggttt tttcgatttg aaagaagatc aaccaccgcg cttcgacttg atttttctcg
     4081 gcctgggcga ggacggtcac acggcgtcgc tgtttcccga cagctcagcg cttcacgaga
     4141 ccgaagcgct ggtggcaccg gtctatgttg aaaaactcaa ggcgcaccgg ctgaccttaa
     4201 ccttgccgac gatcaatagc gccgggcaaa ttagttttct tattgccggg caaagcaagt
     4261 cagccgtcgt aaaatccatc tttaccggcc aaaacaccga agcaccggcg gcgcggatca
     4321 aaccgggcca cggtaagctc acctggttta ttaccgagga cgccgccggc gatctgggca
     4381 aagcttaaac ctgctagata ggcgcccttc cagtcaggat attacaacga tttgattttt
     4441 ctcagttcgg cgacgatctg ctggttggac ggccgtttgt cttgggtttt ttcgatgaag
     4501 cgccagcgca ccacaccctg gggatcgatc acgaaggtcg atgggtaggg ccagcccttc
     4561 ccgtccggat taaaaatccc atagcgatcg atcactttat gatctttgtc ttccaataac
     4621 ggaaagtcga attcgccgtc gaagcgcttg cgcagcgtct cgccgaattt ttttgactcg
     4681 tcgcgggtat cgacgctgac ggcgaggatc tgcaccttgt ctttctgtgc cttggtcaaa
     4741 aggcttttca gcttcccgag ctgttccatg cagaagggtc accagtagcc gcggtagaaa
     4801 accaatacca cggctttctt gccgcgaaag tcggacaact tgatgacctg gccttcgacg
     4861 ttttccaggc cgaagtcggg cgcggcttgt ccgatcttaa tgcggtcgag ttcagtggcg
     4921 ctgccgctgg ttgggtcctt gggcccgagc tgggcgaaaa gcggattcgc cgttaatacg
     4981 ccggcaatca agccggccat caaccatgat gatttcaatt tgttctccca atacaccgaa
     5041 ggacgatacc agagatcata aattccccga gcgattattc aagagcgccg gttcgccggg
     5101 agcgtcacgt agggacgaaa gcgccgctgt gccagatagc gaaacagagc gggcgacagc
     5161 cgctggagcc aacacatcaa agccagccag cgcggcacga tgcgctcgaa tcgatcgcgc
     5221 tcgatgactt gcatcaccgc ctcgctgaca acgccgctgc cgacgaagag cgccggcggt
     5281 gattgcggct cgctgttgtg a
//
LOCUS       DASQKS010000504         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_700847, whole genome shotgun sequence.
ACCESSION   DASQKS010000504 DASQKS010000000
VERSION     DASQKS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700847"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VE170_12995"
     CDS             <1..949
                     /locus_tag="VE170_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16403.1"
                     /translation="GADGDVQQRLLVEAARKEGKMIFYTSVETEFARSLTSAFEAKYP
                     FIKTDIFRSTHDRIFSRMNIERQTNNYVADVVSVGEFETYHMQKKGFLAPYKSPYAAV
                     YAEGFKDPNGYWTDLYDNLIVTAYNTTRVKRDELPKRWEDLLHPRWKGRMVLDQNEDR
                     WFANMLHFMGEKKGLEFMQSLAKQEVAVRSGRSLITQLLSAGEFDLQIVAYWYRPHLM
                     KKQGAPVDWLGLEPAILALHPISIVEKTPHPNAARLFIDFALSDEGQRIFLKRGREPA
                     MPGLRPEGYPGHLKVIPSRVQLAEKLDDYSRQYRALFVR"
     gene            complement(1203..1649)
                     /locus_tag="VE170_13000"
     CDS             complement(1203..1649)
                     /locus_tag="VE170_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16404.1"
                     /translation="MAGEKTVGDITFRIRHTMMLVSDLDRSMDFYTRLLGMDVQRLRP
                     SPDKNERVGYIGYGTEDEYPGLELIETGGPGHVPAMPKWYGHVAIYVSDLASLSDRLK
                     AEGVKFTLGPQPNRPGSQDKVAFIQDPDGYLLELTERHSKSGPPLS"
     gene            complement(1975..2829)
                     /locus_tag="VE170_13005"
     CDS             complement(1975..2829)
                     /locus_tag="VE170_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYJ16405.1"
                     /translation="MMPLRKFSIHQPNTVAEASQMLTQYGEKGRLYAGGTELLLAMKH
                     DLLRYEHLVDVKTIAGLNKIELKNGALMIGGSATHRAIERSPLVKQSLPVLAEMETKV
                     ANIRVRACGTLGGNLCFAEPHSDPATLLTALGAKAHVQGKAGARTLAMDKLISGAYET
                     SLASDELLVGVEVPVLANNQRAAYLKFQLKERPTLGLALFLELDGDSIRKAAAVVGSV
                     SVAPTQSVQANGLLTGGRAQVEKQLGDAAEALAQAADPVDDGEGGADYKRHLIGVFLR
                     RAFIKALS"
     gene            complement(2882..>4146)
                     /locus_tag="VE170_13010"
     CDS             complement(2882..>4146)
                     /locus_tag="VE170_13010"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ16406.1"
                     /translation="RCVGPYAIANVLIENMSLYTNTVPASSYRGFGCAQVTLPGESQI
                     DELAAKIGKDPYQFRLENAAKPGEEFFPGMRPFDGTLKQDLDTAAKAIGWGTPLAKGH
                     GRTVAVSGSDAGAYPLTSTAVRVHADGSATIMTGSTELGQGSHTVLPQIAAEELGIAY
                     GQVNVVSSDTAITPYDRSTGASRTTTLMGSAVLEACQEAIQQMVVMAADVLKVRVDEI
                     QKVRGGVRCGEVELNWSQVISKFYALPDGEVIGRSYIRKSGRFAALPVFWEAAATGVE
                     LSVDEDTGKITMEKLATVGDVGLAINPALAEGQDIGAATMGMGIGLFEELIYEGSQLM
                     NGSILDYRLPRFSDLPKQVELHLVQNRDGVGPYGAKGGGEASLNSMAANIANAVDKAV
                     GVRIRQAPLTPERVWRALKEKKVQGSKV"
BASE COUNT          825 a         1175 c         1249 g          897 t
ORIGIN      
        1 tggcgccgat ggcgatgtac aacagcggtt gctcgtcgaa gcggcgcgca aagaaggcaa
       61 gatgattttc tacacttcgg tggagaccga gtttgcccgc tcgctcacca gcgccttcga
      121 agccaagtat ccatttatca agaccgacat atttcgctcg actcacgaca gaatcttcag
      181 ccgcatgaac atcgagcgcc agaccaataa ctacgtcgcc gacgtggtca gcgtcggcga
      241 gtttgaaacc tatcacatgc agaagaaagg ttttctcgcg ccttacaaat ctccctacgc
      301 cgccgtctat gccgagggat tcaaagaccc caacggctac tggaccgatc tctacgacaa
      361 cctgatcgtc accgcgtata acaccacccg cgtgaaacgg gacgaattac ccaaacgatg
      421 ggaagactta ttgcacccgc gctggaaggg gcggatggtg ttggatcaga atgaagatcg
      481 ctggtttgcc aacatgctcc atttcatggg cgagaagaaa gggctggagt tcatgcagtc
      541 gttggcgaag caagaggtgg ccgtccgtag cggccgctcg ctgatcaccc aactgctcag
      601 tgccggcgag ttcgacttgc agatcgtcgc ctactggtac cggccgcatt tgatgaaaaa
      661 acagggcgcg ccggtggatt ggctgggact ggagccggcg atcctggcgc tgcacccgat
      721 cagtatcgtc gagaaaacgc cgcacccaaa cgcagcgcgc ctgttcatcg atttcgctct
      781 atcggatgag ggccagagaa tttttctcaa gcgcggccgc gagccggcga tgcccggctt
      841 acgaccggaa ggctaccccg gtcatctgaa ggtgattccc agccgggtgc agttagctga
      901 gaagctcgac gattatagcc ggcagtatcg cgctctgttt gtccgttaaa ccggagtcgc
      961 caggcagatt ttaacgggac gggtcattcg cagtgatgcc gatgggcgtc tgactcagcg
     1021 cactgctggg actcgcgcgc aataggtttg ttcggcaggg aagtgagatg ttgacctgcg
     1081 aatgaaacac gactttgcgt cttcacggct cggtggcttc actgctccgg acgcactcct
     1141 tgccggctgc agtgccgttt ctctcctgcg gatttcgggt taaacctatc gaaggtatgc
     1201 gctcaactga gcggcgggcc gctcttcgag tggcgctcgg tcagttctaa aagatagccg
     1261 tcgggatctt ggatgaaggc aaccttatcc tggctgccgg gacggttggg ctgggggccg
     1321 agggtaaatt ttacgccttc ggcttttagt ctatcgctca acgaggcgag atcggaaaca
     1381 tagatcgcga catggccata ccacttgggc atcgccggga catggccggg gccgccggtt
     1441 tcgatcagtt ccaacccagg atattcgtct tctgtgccgt aaccgatata gccgacccgc
     1501 tcgtttttgt cgggcgaggg gcgcagccgt tgcacgtcca tgcccaacaa acgggtataa
     1561 aaatccatgc tgcgatcgag atcgctgacc agcatcatcg tatgacggat gcgaaacgtg
     1621 atatcaccga cggtcttttc tcctgccatc tgttcctcct gaatttcggt agaatacgcc
     1681 agcgcagcca cgcgcttcgg cgctgacact agcccaggac tgagcaggga ttcaagcaca
     1741 gccggcagca gcgcctaaag ggtaagggca atgtttttag cgggagagac gattcgataa
     1801 ccgcgattac caaaacgttt catcagcgga cgctatggac aaaaggtttt tccggacgtt
     1861 gaatgagcgg gagtagttgc cggtcaaaac ataatctccc gcgtccggga agcgaacgtg
     1921 aaatcatccg cgccgttacg ggtggatatg ccagctcggc aagaataaat tttcttaaga
     1981 caacgctttg atgaaagcgc ggcggagaaa cacaccgatc aagtggcgct tgtagtcggc
     2041 gccgccttcg ccatcatcaa ccgggtcggc ggcttgggcc aaggcttcgg cggcgtcgcc
     2101 caattgcttt tcgacctgcg cgcggccgcc ggtaagtaag ccattggctt ggacggactg
     2161 agtcggtgcg acgctgacac tgccgacgac ggcggcagct tttctgatgc tgtcgccgtc
     2221 gagttccaag aacaacgcta gtcccaaggt gggccgctcc ttgagttgga attttaaata
     2281 agcggcacgc tggttatttg ccaataccgg cacctcgacg ccgaccagca gttcatcgct
     2341 ggcaagggaa gtttcatagg cgccgctaat cagtttatcc attgccaagg tccgcgctcc
     2401 ggctttgccc tggacatggg ccttggcgcc gagcgcggtc aataacgtcg ctggatcgga
     2461 gtggggctcg gcgaagcaga gattgccgcc tagggtgccg caggcgcgca cgcggatgtt
     2521 tgccactttg gtttccatct cggccaacac gggcaggctt tgtttgacca acggcgagcg
     2581 ttcgatggcg cgatgggtgg cgctgccgcc gatcattagc gcgccgtttt tcagctcgat
     2641 cttattcaag ccggcaatgg ttttgacatc gaccaggtgc tcgtaacgca agagatcatg
     2701 cttcatggcg agcagcagct cggtgccgcc ggcgtagagc cggcctttct cgccgtactg
     2761 ggtgagcatc tgcgacgctt cggcgaccgt attgggttga tgtatcgaga attttcttaa
     2821 gggcatcata aataattctc gttcaagggt tcggcggctc cgggtttcag tccttgaact
     2881 tttaaacttt tgaaccttga acctttttct ctttgagcgc gcgccaaacc cgttccggcg
     2941 tgagcggcgc ttgacggatg cgcactccga ccgctttgtc gacggcgttg gcgatgttgg
     3001 cggccatgga gttcaacgac gcttcgccgc cgcccttggc gccgtaggga ccgacgccgt
     3061 cgcggttttg caccaagtga agctcgactt gtttcggtaa atcggaaaag cgcggcaaac
     3121 ggtagtcgag gatcgagccg ttcatcaact gcgagccttc gtaaatcaat tcttcgaaca
     3181 ggccgatgcc catgcccatg gtcgcggcgc cgatatcttg gccttcggcc agggccggat
     3241 tgatcgccaa tccgacatcg ccgacggtgg cgagtttttc catggtgatt ttgccggtgt
     3301 cttcgtcgac ggagagttcg acgccggtgg cggcggcttc ccagaacacc ggcagggcgg
     3361 cgaacctgcc ggactttcta atgtacgagc ggccgatcac ttcgccgtcc ggcagagcgt
     3421 aaaatttgct gatcacctgg gaccagttaa gctcaacttc gccgcagcgg acgccgccgc
     3481 gtactttttg aatctcgtcg actctgacct tgagcacgtc ggcggccatg acgaccatct
     3541 gctgtatcgc ttcttggcaa gcttccagta ccgcgctgcc catcagcgtc gttgtccggc
     3601 tggcgccggt ggagcgatcg tagggcgtga tcgcggtgtc cgacgacacc acgttgactt
     3661 ggccgtaggc gatgcccaat tcttcggcgg caatctgcgg caggacggtg tggctgccct
     3721 ggccgagctc ggtgctgccg gtcatgatgg tcgccgagcc gtcggcatgc acgcgcacgg
     3781 ccgttgaggt cagcggataa gcgccggcgt cgctgccgga gacggcgacg gtacggccat
     3841 gccctttcgc caacggcgtg ccccagccga tcgctttggc cgcggtgtct aaatcttgtt
     3901 tgagcgtgcc gtcgaagggg cgcatgccgg gaaaaaattc ttcgccgggt ttggccgcat
     3961 tctcgagccg aaactggtag ggatctttgc cgatcttggc cgccagttcg tcgatctgcg
     4021 attcgccggg caacgtcact tgcgcgcagc caaagccgcg gtaagaactg gccggcacgg
     4081 tgttggtata gagcgacatg ttttcgatta acacattggc gatcgcgtag ggaccgacgc
     4141 aacgat
//
LOCUS       DASQKS010000505         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2807855, whole genome shotgun sequence.
ACCESSION   DASQKS010000505 DASQKS010000000
VERSION     DASQKS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2807855"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VE170_13015"
     CDS             <1..746
                     /locus_tag="VE170_13015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16407.1"
                     /translation="PSIFFNQLQKHQEVKFDWAKFTWIGSSDKSDWLLYMRADLPYKS
                     LADVRRANASPKCGSTGVGTSESYIPKLLEETLGTKFTIVAGYQGGGDIDLAVERGEL
                     QCRALTVQAFHSREPYHGWRKNNFTRIVMQTGRVRDERLPDTPTLAELMDEHKTSAAA
                     RRLVPVVLASGDFGRPIIAPPGVAPDKIKILRDAFMKTMNDPELLAEAKKKSLSITPT
                     RGEELEKAAKAVVVQSPEMLQRLKTLMNE"
     gene            complement(1049..1309)
                     /locus_tag="VE170_13020"
     CDS             complement(1049..1309)
                     /locus_tag="VE170_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16408.1"
                     /translation="MKLSLKAMIIAGALFKTVVFLFISLMNLILRPYGGAYLAILTSL
                     YPGYDPVSVPIAIVVGTLYSLLAGALAGLLFGCLYNFFTEHN"
     gene            1429..2652
                     /locus_tag="VE170_13025"
     CDS             1429..2652
                     /locus_tag="VE170_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16409.1"
                     /translation="MAEILSCNLARVQPRKGKILSLLSIDIHGSEEILPIKVSSNDCV
                     MILREITLEVAVKKLAVMIFLTMLLGLGLPGQGFSQSPFYQGKTINVVYGGGPGGTVD
                     LRFRATASVLRKHIPGRPTFIIEYMPGGGSRKAGNYIYRARPDGLTMGVMLSSFVPAA
                     VLDSTGVLYDINKLSYLGSPTSGEPYIFLSRKEARLDSMEKIRSASGVRIGAQSVGHN
                     LYNSSRILAYLVAMKEPKFVTGFAQGHEVNLALMQGELDARWTIQATLVREAPEWIDK
                     KLMDFHVVNEVPKGRRHPHPVFAQLPDIERFTKSEKDRKLLAMQRAFVSAGTPFILPP
                     GTPPDKVQILREAMRKTFADPEFYAEFRKLVGEEPTPLPPEELEKAIRDLPRDREVVE
                     LFNKLSGADAMPVAP"
     gene            complement(2929..3765)
                     /locus_tag="VE170_13030"
     CDS             complement(2929..3765)
                     /locus_tag="VE170_13030"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16410.1"
                     /translation="MATNVEYQVEERDVEYQRQAGKPWLARIYQPRGSGPFPTMVDVH
                     GGAWHNGDRTNNAGVDRALAAKGVVVAALDFHQPPEAGYPASICDVNLAVRWLKAHAA
                     EFNGTTAVGAFGNSSGGHQVVLSALRPRHAAYSTLPLPNHPEIDASIAYVIAAWPVIC
                     PLYRFRFAKEFNREEHINAHIEYWGNEDAMAEGSPQTILDKGEKAELPPILFLMKAND
                     KNHPLEMQERFITSYRKRGGAIEVDTFDGLPEHRMVPSPSEPETMRFIEVVSAFIRQQ
                     SK"
     gene            3972..5534
                     /locus_tag="VE170_13035"
     CDS             3972..5534
                     /locus_tag="VE170_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ16411.1"
                     /translation="MNIRELVLTPQEAFDEALFIEVLSKKLSIPRDGSLTVLPLKRSV
                     DARGRNIVVRVQYDIVERASIPPRRTKDYPSVHGKNPVVIIGSGPAGLFAALRSIELG
                     YKPIVLERGKDVQARRYDVAAINKKHSVNPDSNYCFGEGGAGTYSDGKLYTRSKKRGD
                     VQRILEILVAHGAKDEILFDAHPHIGTNKLPKLVAELRESIKQAGGEVHFETRVTDFV
                     LAGNKLTGVRTQHGEIYEGRATILATGHSARDIFHLLNDKKILIEAKPFALGVRVEHR
                     QRLIDTLQYHCRGDRGAYLPAAAYALVHQAKINGKDKGVFSFCMCPGGFIVPSATAPG
                     ELVVNGMSPSGRNARFANSGIVVAVDENDYHHLRRYGPLAAMQFQAEIEQKAWAVAGK
                     SQSAPAQRLIDFVENRVSASLLDTSYHPGLTSSDMRAFLPEFVADGLRQGFKSFGQKM
                     PGYLTNDAQIIGVESRTSSPVRIPRDKISLHHPQIANLFPCGEGAGYAGGIVSAAMDG
                     ERCAEAAMRLLK"
     gene            5619..>7036
                     /locus_tag="VE170_13040"
     CDS             5619..>7036
                     /locus_tag="VE170_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HYJ16412.1"
                     /translation="MMHTPNTAGTGRFAFDNTYARELEGFYVPWKAAQVARPELVKLN
                     RELAEELGLDADALDSEQGARIFAGNETPEGALPLAQAYAGHQFGGFSPQLGDGRALL
                     LGEVIDRHGQRRDIQLKGSGPTPFSRAGDGRAALGPVLREYLIGEAMQALAIPTTRAL
                     AAVLTGEPVYREHALPGAVLTRVAASHIRVGTFQLFAARGEHEKVRRLADYVIARHYP
                     ELKDQANPYLGLLVSVCDRQAALIASWMHVGFIHGVMNTDNMAISGETIDYGPCAFMD
                     HYDPATVFSSIDTRGRYAYGNQPSIAQWNLARLAETILPLIDADSDRAVAHATEVVND
                     FPHRYERIWLQGMRAKLGLVGEDEADLNLAQGFLTAMANNNVDYTLAFRYLADAALGD
                     EEPIRALFADASAYDLWSEHWRARLARETLAPAERAQAMRRVNPAFIPRNHRVEEALS
                     AAVERADYAPFEVLLHILARPF"
BASE COUNT         1546 a         1991 c         1998 g         1501 t
ORIGIN      
        1 aaccgtcgat attcttcaat cagctccaga aacatcaaga ggtaaaattc gactgggcaa
       61 agttcacctg gatcggcagc tcggacaagt ccgattggct gctctatatg cgcgccgatc
      121 tgccttacaa gagcctggcc gacgtgcgtc gggctaatgc ttcgccaaaa tgcggttcca
      181 ccggcgtcgg cacgtcggaa agttatattc cgaagctttt agaagaaacc ctggggacaa
      241 agtttactat cgtggccggc tatcaaggcg gcggcgatat cgacctagcc gtggagcggg
      301 gagagctcca atgccgggcg ttgaccgtcc aggcttttca ttcccgcgag ccctatcatg
      361 gctggcgtaa aaacaacttc acccggatcg taatgcagac cggacgcgtg cgcgacgagc
      421 gcttacccga tactcctacg cttgccgagc tcatggacga gcacaaaacc tcggccgcgg
      481 cacgccgatt ggtgcctgta gtcttggcga gcggcgattt cggccggccc attatcgcgc
      541 ctcccggagt agcgccggac aaaatcaaaa ttttacgcga cgcttttatg aaaactatga
      601 atgatcctga actcctggcc gaggcgaaaa aaaagagctt gagcattacg ccgacgcgcg
      661 gcgaggagct tgaaaaggcg gctaaggctg tcgtcgtgca gtcgcccgag atgctgcaac
      721 gattgaaaac gctgatgaat gagtaaaata gatgcggggt tttttcctgt aattcactgc
      781 cgaacgagca aagtgatttt tcttccaaat agatttaagt gaaatagcgc tgtaagctat
      841 gggttcgcaa agcttgttgc gcgttacccg aaagccgcgc gctaccgata tctcacctct
      901 ttcgatccgc agagttgaat cacggcaaac agctacgtga tgacggatgc aatccctgcc
      961 ggcgtcagcg aaagtgccgt cggcgatccc aagagcgcat ccgcaaacta tcgctcctgg
     1021 ctcgcggagc cggggctttc ccctggtgtt aattatgttc ggtgaagaaa ttgtaaaggc
     1081 agccgaacaa gagaccggcg agcgcgccgg cgagtaagga atagagcgtg ccgacgacta
     1141 tggcgatggg cacgctgact gggtcgtaac cgggatagag cgaagttaaa atcgcgaggt
     1201 aggcgccgcc gtaaggacgc aagatgagat tcatcagcga gataaaaaga aagacgacgg
     1261 ttttgaatag cgcgccggcg atgatcatgg ccttgagtga cagcttcata gtgcaccatc
     1321 cacaacattc ctatagtcga ttttcagctc ccaacgctac ttcgcagtta cccttagtta
     1381 gaatgtcaga cagcaagtca aaatgccaag gttgacattg acatccgatt ggcggaaatt
     1441 ctctcctgca atttggcaag agtgcagcct cgcaagggca aaattttatc tttgctctcg
     1501 atcgacattc atggaagcga agagatatta ccgataaagg ttagttcgaa cgactgcgtc
     1561 atgatcctcc gtgaaatcac gctggaggtt gccgtgaaaa aacttgctgt catgatcttc
     1621 ttgaccatgc tgttgggcct cgggcttccc ggacagggat tctctcagtc tcccttttat
     1681 caaggcaaga ccatcaatgt cgtctatggg ggcggacccg gaggcacagt cgatttgcgt
     1741 ttcagagcca ccgcttcggt tctgcgcaaa cacataccgg gtagaccgac tttcatcatt
     1801 gaatatatgc caggcggtgg atcgcgaaag gctggaaact acatctaccg cgctcgaccg
     1861 gacggattga ccatgggcgt tatgctctca agctttgtac cggcggctgt gctcgactcc
     1921 accggggttc tctatgacat caacaaacta agttaccttg gatctcctac cagcggcgaa
     1981 ccatatattt ttttgagccg taaggaggcg cgactggata gcatggagaa aatccggagc
     2041 gcctcaggcg tgcgtatcgg cgctcagtct gtgggtcata atctctataa cagcagccgc
     2101 atccttgcgt atctggtcgc catgaaagaa ccaaagtttg ttaccggatt cgcccaaggt
     2161 cacgaagtca atctagcctt gatgcaaggc gagctcgatg cccggtggac cattcaggcc
     2221 actctggtac gagaagcacc cgaatggatc gacaagaaac tgatggattt tcatgtcgtc
     2281 aacgaagtgc ccaaggggcg aagacacccc catcccgtct tcgcccagct gcccgatatc
     2341 gaacggttta cgaaatcgga gaaggaccgg aagcttcttg ccatgcaaag ggcctttgta
     2401 agtgcgggaa cgccgttcat tctgcctcca ggaacacccc ccgataaggt gcaaatcctg
     2461 cgggaagcga tgcggaaaac gtttgctgat cctgaattct acgcggaatt tagaaagctt
     2521 gtcggcgagg agcctacgcc gttgccgccg gaggaactgg agaaggcaat tcgtgacttg
     2581 ccccgggatc gggaagttgt cgaattgttt aacaagctct ccggcgcgga cgctatgccg
     2641 gtggcaccgt aggctaagtc gcttccacag tcgcatcagc gctaacaaga atacgagttt
     2701 ttgttgaaac gtttggggtc gaaccggcat tgcgctggcg cactcagatc tactacgttg
     2761 gcttgtagtc gacagatttt accagagcgg gaaaatcttt cgtgaggcgc agctctgata
     2821 cgctatgctg ccttgtgcaa cgacgcgcct taccaatggt ttgacaatcc cctctgaggc
     2881 acgcgcgctc gcgaccggcg agataaagaa ttatgacgcc cggcgcgtct atttgctttg
     2941 ctgccgaatg aatgcgctga caacttcgat gaaccgcatc gtttccggct cgctcggcga
     3001 cggcaccatg cggtgctccg gcaggccgtc aaaggtatcc acctcgatgg cgccgccgcg
     3061 tttgcggtag gaggtgatga agcgctcctg catttccagc ggatggtttt tgtcgttcgc
     3121 cttcatcaag aatagtattg gcggcagttc ggccttctcg cccttatcca ggatcgtctg
     3181 cggactgcct tcggccatcg catcttcgtt gccccaatac tcgatatgcg cgttgatatg
     3241 ttcttcgcgg ttgaattcct tggcaaagcg gaagcgatac agcgggcaga tcaccggcca
     3301 agcggcgatt acataggcga tgctggcatc gatctcagga tggttcggca gcggcagcgt
     3361 cgaatacgcc gcgtgccgcg gccgcagcgc gctaagtacc acttgatggc cgccgctgga
     3421 attaccaaac gcaccgacag cggtggtgcc gttgaactca gccgcatgcg ccttgagcca
     3481 tcgaactgcg agattcacat cgcagatcga cgcgggataa cccgcttccg gcggctgatg
     3541 aaaatcgagc gccgccacga ccactccctt cgccgctagc gcgcggtcga caccggcgtt
     3601 gttggtccga tcgccgttgt gccaggcgcc gccatgcacg tcaaccatgg tcggaaacgg
     3661 cccggatcct ctgggttgat aaatgcgcgc gagccaaggc ttgccagcct ggcgctggta
     3721 ctcgacatcg cgctcttcga cttggtattc aacgtttgta gccatggcgg gaatctactg
     3781 ccgcacgggg tcgctgtgca agtccaacga agcactgcca gataatacaa ttaccggccg
     3841 cactcgtttg gccaacttta cgctcctaca ccgaatgctt gagcgttccg atttttttta
     3901 tgtcgcgtgt tatagttacg tgcaataaca ggtgcgtaac cacaaaattg caggataaaa
     3961 acctgccggg tatgaacata agagaactgg tcctgacgcc gcaggaggcc tttgacgaag
     4021 ccttattcat tgaggtcttg tctaagaagc tgtcgattcc tcgcgatggc agtttgactg
     4081 tgttgccgct caagcgctcg gtggacgcgc gcggccgtaa catagtcgtg cgcgtgcaat
     4141 atgacatcgt cgagcgcgct agcattccgc cgcggcgcac caaggactac ccttcggtcc
     4201 atggcaagaa tccggtggtt attatcggct caggtccggc cggtttgttc gccgcgctcc
     4261 gctcgattga gttgggctat aaaccgatcg tgctggagcg cggcaaagac gtccaagcgc
     4321 ggcgctacga tgtcgccgcc atcaataaga aacatagcgt caatcccgat tccaactatt
     4381 gcttcggcga aggtggcgcg ggcacttatt ccgacggcaa attatatacg cgttccaaga
     4441 agcgcggcga cgtgcaacgc attttagaaa tattggtagc gcacggcgcc aaggatgaaa
     4501 tcctcttcga cgcgcatccg catatcggca ccaacaagct gccgaaactg gttgccgaac
     4561 tgcgcgagag catcaaacag gcgggtggag aggttcattt cgaaacccgc gtgactgatt
     4621 tcgtgctcgc gggaaataag ctgaccggcg tgcgcaccca gcatggcgaa atctacgaag
     4681 gccgggcgac gattttagcc acgggccact ccgcccgtga tatttttcac ttgctgaacg
     4741 ataagaagat tctgatcgaa gccaagcctt tcgccttggg cgtgcgagtc gagcatcggc
     4801 agcgcctcat cgatactctc caataccatt gtcggggcga cagaggcgcc tatttacccg
     4861 ccgccgctta tgccttggtg catcaggcga agatcaacgg caaggataaa ggcgtgtttt
     4921 ccttttgcat gtgcccgggg gggtttatcg tgccgtcagc gactgcgccg ggagagttgg
     4981 tggtcaatgg catgagccct tctggaagga atgcgcgctt tgccaactca ggcattgtcg
     5041 tggccgtaga tgaaaacgat tatcaccatt tgcgccgcta tggtccccta gccgccatgc
     5101 agttccaggc agaaatagaa caaaaagcct gggccgtggc cggcaagtca caatccgccc
     5161 cggcccaacg tctgatcgat tttgtcgaaa acagagtgtc ggccagtttg ctcgacactt
     5221 cgtatcaccc cggtttaacg tcaagcgata tgcgcgcgtt cctccccgag tttgtcgccg
     5281 acgggctgcg tcaggggttc aaaagcttcg gtcaaaaaat gccggggtac cttaccaacg
     5341 acgcccaaat catcggagtg gaaagccgca cttcctcacc ggtgcgcatc ccccgcgaca
     5401 agatcagctt acatcatccg cagattgcca atttattccc ctgcggtgaa ggcgccggtt
     5461 acgccggcgg cattgtctcg gcggccatgg atggggagcg ctgcgccgaa gccgccatgc
     5521 gactattgaa ataatcgacg ccggcagctg cttcagacat tgcctgtcgc tgggcgcccc
     5581 aacctgttaa gggtaaaatc ttcgtggtaa attgttcaat gatgcatact ccaaacaccg
     5641 ccggaaccgg ccgcttcgcc ttcgacaaca cctacgcgcg tgaacttgag ggcttctacg
     5701 tgccctggaa ggcagcgcag gtggcgcggc ctgagttggt taaactcaat cgcgagttgg
     5761 ccgaggaatt ggggctcgac gccgacgctc tcgattccga acaaggtgca cgaatctttg
     5821 ccggcaatga gacacccgag ggagcgctgc cgttggctca ggcctacgcc gggcaccagt
     5881 tcggcggctt ctcgcctcaa ctcggcgatg ggcgggcgct gctcctgggc gaggtgatcg
     5941 accgccacgg ccagcgccgt gacattcaac tcaagggctc ggggcccacg cctttctccc
     6001 gcgcaggcga cggccgcgcc gcgctcggcc cggtgttgcg tgaatacttg atcggcgaag
     6061 cgatgcaggc gcttgctatc ccgaccacac gggcgcttgc cgcagtgctc acgggcgagc
     6121 cggtctatcg cgagcatgcg ctgcccggcg cggttttgac acgagtcgct gccagccaca
     6181 ttcgtgtcgg cacctttcag ttgttcgccg cccgtggcga acatgagaag gtgcgtcgcc
     6241 tggccgatta tgtgatcgcg cgccattatc cggagttgaa agatcaagcc aacccttacc
     6301 ttggattgct tgtcagtgtg tgcgacaggc aagcagcgct gatcgctagc tggatgcatg
     6361 tcgggttcat ccacggcgtg atgaacaccg acaacatggc gatttccggt gagaccatcg
     6421 actatgggcc atgcgccttc atggatcatt atgacccggc caccgtgttc agctccatcg
     6481 acacgcgcgg ccgctacgcc tacggcaatc aaccaagcat cgcgcaatgg aacctggcgc
     6541 gattggcgga gacgattttg cctctgatcg acgccgatag tgaccgcgcc gtcgcccacg
     6601 ccaccgaggt ggtcaatgat ttcccccatc gatatgaacg catttggctt caaggcatgc
     6661 gcgccaagct cggcctggtc ggtgaagatg aggccgatct caatctcgcc caggggtttc
     6721 tcaccgccat ggcgaacaac aacgtagatt acacattagc cttccgctat ctcgccgacg
     6781 cggccttagg agatgaagaa ccgatacgcg cgctgtttgc cgatgcgtcg gcctatgatc
     6841 tctggagtga acactggcgg gcgcggcttg cgcgcgagac ccttgcgcct gcggaacggg
     6901 cccaagccat gcggcgggtc aatcccgcgt ttattccgcg caatcatcga gtagaggaag
     6961 ccctgagcgc cgcagtggaa cgtgccgatt acgcaccgtt cgaggtgctg ctgcatattc
     7021 tagcgcgacc cttcga
//
LOCUS       DASQKS010000506         8271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1952290, whole genome shotgun sequence.
ACCESSION   DASQKS010000506 DASQKS010000000
VERSION     DASQKS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952290"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VE170_13045"
     CDS             <1..794
                     /locus_tag="VE170_13045"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16413.1"
                     /translation="GNYPFVGIPAFVYRKFRHSCAYVNVNAGIDKPQDLKGKRVGIPE
                     WQMTATVWLRGFLQDDYGVKPSDIQWLTGGLESSERKEKVALKLPPEIKIENIPAGKN
                     LSDMLVTGEIDALLTAQVPAPYVKRVTQVRRLFADPRAVETDHFRRTGIFPIMHVIVL
                     REELVRRHPWIAQSLYKALLDAKQNCIDGIFRNDAIHSVIPWAANHAEEMRQLMGDDF
                     WPYGVESNRQTLETFLRYADEQALTEHKLSIDELFPRQTLDTFKV"
     gene            1267..1473
                     /locus_tag="VE170_13050"
     CDS             1267..1473
                     /locus_tag="VE170_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16414.1"
                     /translation="MKTTDNALDKPSYWQRVFAQSKVNGGMYFGEELPGRNWSWPTDT
                     YLQRRLRRRLQRDNQSQTSSSNLN"
     gene            complement(1700..2140)
                     /locus_tag="VE170_13055"
     CDS             complement(1700..2140)
                     /locus_tag="VE170_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16415.1"
                     /translation="MSGIHKLWAAPLFAFLLSGLSFGAETSSTKEPPKESAKTAPVKK
                     KATKPKKPKVQKFVGNISSVDAKTGTVSVKGSAGEKSFITQDAAKDALERLAVGDRVR
                     IQYSERDGKLAATSVRRVKLPQTKTKPSAPNPKATEQKNQPKKL"
     gene            complement(2195..3613)
                     /locus_tag="VE170_13060"
     CDS             complement(2195..3613)
                     /locus_tag="VE170_13060"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16416.1"
                     /translation="MSYKDVREWIEKVQEMGELKIVEGADWNLEIGALSVLASKHKEN
                     SPALLFDKIKDYPAGYRILVGFFESLKRSALTTNLPLDISRDGFIKAWRERLARPASI
                     PPVTVSSGPILENIFDGDAIDLFKFPVPFWHERDGGRYIGTGHVVITRDPDDGWVNVG
                     CYRVMVHDRNTMGMNISPGKHGRMHRQKYFDRGKPCPVVCCFGVDPLLHMFATRPEPY
                     GRSEFDAVGGIKGEPMEVITGELTGLPIPAYAEIAIEGEFLPDQSKEEGPFSEWTGYY
                     ASGEKMEPLIKVHRLYHRNDPIITASPEYRPTARAEHCYELLRAAYIRDQVEKAGVPD
                     VTAVASYFRRFLTVIAIQQRYPGHARQAGLIASQCQGSAYLGRYIVVVDEDIDAYDIN
                     DVLWAMCSRADPVDSAEIIRRCWSGPLDPIIPSERKGFSSRMIVDACRPFEWRDKFPP
                     AAHISPERQQAVIAKWKEQLFT"
     gene            complement(3782..4981)
                     /locus_tag="VE170_13065"
     CDS             complement(3782..4981)
                     /locus_tag="VE170_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16417.1"
                     /translation="MALGDQRSPDGRFRAAERMFLLGALLLLGLVVLCGSLLLVRHEQ
                     QYVRLAESFLSGKLHLLVELPEHLHDTAFYGGRYYWPLGPFPAVVLMPFVAICRWIGV
                     EFFQAYASVPLSLWTSWLIFRMASKCGRSKVESAWLVLAFCGASSYPSVAAISMSWPF
                     AHVVAIHLLFLALHEWLGRRRWLFIGFVLGLTSATRLSAGLNIILFAAAALMTEPPLR
                     KKASVALAMGFALPMVLLASYNFARFDSFVETGYGFQLPGPGDFPSTSLANVLPHLRL
                     FLFGAPVLSDQFPFFITQLVGMSVFLLSPWLLYLASLKFDRVTVVALLNCLIVLVAVL
                     AWRSTGQFQLGYRFSLDFLPVVAFLIARDGFGRAPMPLGFKSLAALGFLSTLYFLWSL
                     IGIMPRR"
     gene            complement(5088..6038)
                     /locus_tag="VE170_13070"
     CDS             complement(5088..6038)
                     /locus_tag="VE170_13070"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16418.1"
                     /translation="MRQIRCCTVGLFLALAVGVSQSASAAERFYISIPGPTLSYAHLY
                     YGQERGFFTQEGLDLQVLVVRGVIGLSSLMSGEIDVTCHAGSGLAAALRGLPIKIVNV
                     THDRPVHDLVVHPSIATPADLKGKAIAVGSLEGTAAVIARRILQAKGLDAQKDVTLLS
                     MDSPARLQSLLSGRVAAGLVTPPTTYLAMDRGYRTMARGRDHIRYLQTGVVATDAAIK
                     QKRAGIVRFLRGWNRALKFYQDNPEIMIPYIQKKLAVSDAKMARRMYDEDRDIISLTG
                     ILTQDATREILDTAREALRIKEPVAVEKVFDFSLASEAQR"
     gene            complement(6074..7804)
                     /locus_tag="VE170_13075"
     CDS             complement(6074..7804)
                     /locus_tag="VE170_13075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYJ16419.1"
                     /translation="MKSTLKSNLIAVLLVSLGCSGREADFARSLAVKSAADTVLRGGK
                     IVTVDGAFSIKQAVAIRDGRFVAVGSDSEMRDYIGAHTQVIDLAGRTAIPGLNDSHIH
                     ATVAGLSWDAELHWQLHRTLADGLRQIATATKSQAPGSWIVVGGGWVPTQLAERRFPT
                     RAELDAIAPNHPVYIQYLRQGALLNTKAFATLGITSKTADPPGGRIDKDPATGIATGW
                     LQGVAAWQLAYNKIPRLTLAQARQSLRNCFHELNRLGITSVSDLHTGGVTFAHRRLLF
                     DMARTDDLSLRFNYYIAPTESGDELEQLRLAVEEIKSLGQHELFRFAGFAETLVRGSG
                     DGDVLSNPQGVQLSPEVKEKFRRVMRYLAETGHSFHLHATQDNTARQLLTVIEQVFTA
                     APPAPQRMIFAHLEDATAETLARIKNLGGGVAVQDRLALTAERNVELWGEAKARRAPP
                     LRTMLDSGVPVGAGTDAFRSASYAPMLSLWWLITGKTVAGSAIREGSQNVSRAEALRM
                     YTMGSAWTTADEERKGSIEVGKFADLAVLTADYLSVPEEQIRNVVSLLTMVGGRIVYA
                     AGPYAALAAK"
     gene            7982..>8271
                     /locus_tag="VE170_13080"
     CDS             7982..>8271
                     /locus_tag="VE170_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HYJ16420.1"
                     /translation="MQPVQLFCWHFMAYPYLDKDFDKSGHSGWVTTPNKLWDNNQARG
                     LYQEYIDQLALADELGFDGMVLNEHHQNIYGLMPSPNIIASALTQRSSRGKIV"
BASE COUNT         1873 a         2439 c         2270 g         1689 t
ORIGIN      
        1 gcggaaacta tccgttcgtc ggcattccgg cattcgtcta tcgtaagttc cgccactcct
       61 gcgcctatgt caatgtcaac gccggcatcg ataaacctca agacttaaaa ggtaaacgcg
      121 tcggtatacc cgagtggcag atgaccgcca cggtctggct gcgtggcttc ttgcaagatg
      181 attacggcgt gaaaccgagc gatatccagt ggctcaccgg tggactagaa tcgtcggagc
      241 gaaaagaaaa agtcgcactt aaactgccgc ctgagatcaa gattgaaaat attcccgccg
      301 gcaaaaacct ttccgatatg ttggtcaccg gcgagatcga tgcgctatta accgcccaag
      361 tacccgctcc gtacgtcaag cgcgtaaccc aagtgcgccg gctatttgcc gatccgcggg
      421 cggtcgagac cgatcatttt cgccgcaccg ggatctttcc gatcatgcat gtcatcgtgc
      481 tgcgcgagga actagtccgg cgccatccct ggatcgctca gagcctttac aaagccttgc
      541 tcgacgccaa gcaaaactgc atcgatggga ttttccgcaa tgacgcgatt cattcggtga
      601 ttccctgggc ggccaatcac gccgaagaga tgcgccaact gatgggcgat gatttttggc
      661 catacggggt cgagagcaat cgccagactc tggaaacatt tttgcgctac gccgacgagc
      721 aagctcttac agaacacaaa ctgagtattg acgagctgtt cccacgtcag accctcgaca
      781 ctttcaaggt gtaacttgtt tcattacaga tgatgtctgt atcccttcgt tgacactgaa
      841 cgggactcga tttacctgcc ggcaacctcc ggtttcatcg tcgccgttgg cgctgatata
      901 gccccatccg ccggcaagat cttcgggtat tgagccgagt atattatcca agcctcgcca
      961 taagtcttgg attactctgc agtttcccgg tcttgccgat aaaaccgccg gccgattccg
     1021 gtgcgcggcc gcgtggcact gttgaccgaa cccgggtttt ggtccagctt gagcggtctg
     1081 ccgtgcccgc cggttcggga ctcaatttga gaaaattcac gatagtctga agctgctggg
     1141 cattttcgac ttcaggcacg gctcctgcac tatgcgtgta atcacaatcg gcaaacggcg
     1201 acgggtatga aacacggggc tgggctattt agacgacaca tcaaggagaa aaaggagaaa
     1261 cggaatatga aaactacgga taacgcgctc gacaaaccga gttactggca acgagtattc
     1321 gcgcagagca aagttaacgg cggcatgtac ttcggcgaag aacttccggg ccgcaactgg
     1381 agctggccca cggacaccta cctgcaacgc cgcctgcgac gccgtctgca acgcgacaac
     1441 caatctcaaa ccagcagctc aaacctgaat tagcttgccg cctcccgcgc tgcctccttc
     1501 agcatagcta ccaggggacc agcggcgcca ctgagccccc atcagctcac tggcaagagg
     1561 ccggattcgc gagagtccgg cctcttattt tttaccgatt tagagcaaga tttttacctg
     1621 taacgcaccg cgaattattc ggcgtgaagg attggcagcg agcgccgtat tcgttggcgc
     1681 gataaacttg gagattactt cacagcttct ttggctggtt cttctgctcc gtcgccttcg
     1741 ggtttggcgc actgggcttg gttttagtct gaggcaattt cactcgccgc accgacgttg
     1801 ccgcgagctt gccgtccctt tccgaatatt gaatccgcac gcgatcgccg accgcgagcc
     1861 gttccaacgc atctttggca gcgtcctgag tgataaaact tttctcgcca gccgagccct
     1921 taaccgatac cgtgccggtc ttggcatcga cggacgatat attgccgaca aatttttgca
     1981 cttttggctt tttgggcttg gtcgccttct ttttaactgg cgcggtcttg gcgctttcct
     2041 ttggcggctc cttggtcgac gacgtctcag cgccaaatga caaccccgac aacaaaaacg
     2101 cgaacaacgg cgccgcccac agcttgtgaa ttccactcat gatcgagctc ccccgaaagc
     2161 ttagacgatc gttattgcca acttatgtca cgttttaagt aaacagctgc tctttccatt
     2221 tggcaatgac cgcctgctgg cgctccgggc tgatatgcgc ggcgggtgga aacttgtcgc
     2281 gccactcaaa cggccggcaa gcatcgacga tcatgcgtga gctgaacccc ttgcgctcac
     2341 tagggatgat cggatcgagg ggaccgctcc agcagcgccg gatgatttca gcggagtcga
     2401 cgggatcggc gcggctgcac atcgcccaaa gcacatcgtt gatatcgtag gcgtcgatat
     2461 cctcgtcgac aacgacgatg tagcggccga gataggcgga gccctggcat tggctggcga
     2521 tcaggccggc ttggcgcgca tgcccgggat agcgttgctg gatcgctatg accgtaagaa
     2581 agcggcgaaa atagctggcc acggcggtga catccggcac gccggctttt tccacttgat
     2641 cgcggatgta agccgcgcgc aacagttcat aacagtgctc cgcccgggcc gttgggcgat
     2701 attccggcga ggcggtaatg atcggatcgt tgcgatggta gaggcgatgg actttgatca
     2761 gcggctccat tttctcaccg ctggcgtaat agccggtcca ctcgctgaac ggaccttctt
     2821 ccttactttg gtcaggcaaa aactcgcctt cgatggcgat ctccgcgtag gcgggtatcg
     2881 gcagtccggt caactcaccg gtaatcactt ccatcggctc gcccttgatg ccgccgacgg
     2941 catcgaattc gctccggcca tagggttcgg ggcgggtggc gaacatatgc agcagcgggt
     3001 cgacgccgaa gcagcacacc acgggacagg gcttaccgcg atcgaaatat ttctgccggt
     3061 gcatgcgacc atgcttgccg ggagaaatgt tcatgcccat ggtgttacgg tcatggacca
     3121 tcacgcgata gcagccgacg ttgacccatc cgtcgtcggg atcccgggta atcaccacat
     3181 ggccggttcc aatatatcgt ccgccgtcgc gttcatgcca aaacggcacg ggaaatttaa
     3241 aaagatcgat ggcgtcgccg tcgaaaatat tttccagaat cggtccgctt gagacagtca
     3301 ccggtggtat cgacgccggc ctggccaagc gctcgcgcca ggctttgatg aacccatcgc
     3361 ggctgatatc gagcggtaaa ttggtcgtca acgccgaccg tttgaggctc tcgaagaagc
     3421 ccaccaggat acgatagccg gctgggtagt ccttgatctt atcgaagagt aaggccgggc
     3481 tgttttcttt gtgcttggaa gctaataccg acaacgcgcc gatttcaaga ttccaatcgg
     3541 caccctcgac gatcttgagc tcgcccatct cttggacttt ttcgatccac tcacgcacat
     3601 ctttatagct catgcttgaa atctcccagc cggtcaattc aagccagcag tacgagtttc
     3661 aatccggctt tgcaagcatg actcgcaaat gttccatccc atggcggtga aacgccacga
     3721 ccggacttag cgccggctgc tcgatgaacg aatcacgcca ttgattgtga aagaacagac
     3781 ttcatcgtcg cggcattatc ccgataagac tccataaaaa ataaagagtg gacaggaaac
     3841 ccaatgcggc gagggatttg aatcccaatg gcattggtgc gcgaccgaag ccgtcacggg
     3901 ctatcagaaa ggcgacgacc ggtaggaaat ccaacgaaaa ccgataaccc aactggaact
     3961 gacctgtgga ccgccacgcg agcactgcga caagcacgat caagcagttt agcagagcta
     4021 cgacagtgac gcggtcgaac ttgagcgatg ctaaatacaa aagccagggc gaaagcagga
     4081 atacgctcat gccgaccaat tgggtgataa agaagggaaa ttgatctgac agcaccggcg
     4141 ctccgaaaag aaaaagtcgc aggtgcggca acacgttggc gagagatgtc gacgggaagt
     4201 cacccggtcc cggcaactga aacccgtagc cggtttcgac aaacgagtcg aagcgcgcga
     4261 agttgtatga agccagtaac accattggca acgcaaagcc catcgccagc gccactgatg
     4321 ctttttttct caacggcggt tcggtcatta acgcggctgc ggcaaagaga atgatattca
     4381 aaccagcgga cagccgggtg gccgacgtca aacctaggac aaatccgatg aaaagccatc
     4441 ggcgcctgcc gagccattca tgaagtgcaa gaaagagcag gtgaatcgcc accacatggg
     4501 caaatggcca actcatcgag atcgccgcca cgctgggata ggatgacgca ccgcagaagg
     4561 cgagcacgag ccaagccgac tctactttac tgcgcccgca ctttgacgcc atgcgaaaaa
     4621 tgagccagct agtccagagc gatagcggca ccgacgcata ggcctgaaaa aattcgacgc
     4681 cgatccagcg gcagatcgcc acaaaaggca tcagtaccac cgcggggaac ggccctagcg
     4741 gccaataata ccgaccgcca tagaacgcag tgtcgtggag atgctcgggc aactcgacaa
     4801 gcaaatggag tttgccgctg agaaaagatt ccgccagccg gacatattgc tgctcatgac
     4861 gaaccagcaa taagctgccg catagcacca ctaagcccag cagcaataga gcgccaagca
     4921 agaacatccg ctccgccgcc cggaatcgac catccggcga ccgctgatcg cctagcgcca
     4981 tattccgatg tttctagtga ttggggcggg caagatggca agcgcgccgg cgctggctag
     5041 aatgggatca tgacggtgga tgaagtgcac ggcgacaact atagcgatta gcgttgcgct
     5101 tcgctcgcca gcgaaaaatc aaaaaccttt tccaccgcca ccggctcttt gatgcgcagc
     5161 gcttcccggg cggtatcgag aatttcgcgt gtggcatctt gagtgagaat tccggtaaga
     5221 gaaataatat cgcggtcctc atcgtacatg cgccgcgcca ttttggcatc gctgacggcc
     5281 aatttttttt gaatgtaggg aatcatgatt tcgggattgt cctggtaaaa tttgagcgcg
     5341 cggttccagc cccggagaaa gcgcacgatg ccggcgcgct tctgtttgat cgccgcatcg
     5401 gtcgccacca cgccggtttg caaatagcgt atatggtcgc ggccgcgtgc catggtgcgg
     5461 taaccgcggt ccatcgctag ataagttgtc ggcggcgtca ccaatccggc ggcgacccgg
     5521 ccgctcaaga gcgactgcaa ccgtgccggt gaatccatgc tgaggagggt cacatctttc
     5581 tgggcgtcta atcctttggc ttgcaatatc cgccgtgcga tcaccgcggc ggtaccctcc
     5641 agcgagccga cggcgatcgc tttgcctttc aggtccgccg gcgtggcgat cgatgggtga
     5701 acgaccaggt cgtgcaccgg acggtcatgg gtgacattga caatcttgat cggcaagccg
     5761 cgcaacgccg ccgccagacc gctgccggca tggcacgtga cgtcgatctc gccggacatc
     5821 aagctgctca agccaattac gccgcgcacc accaaaacct gcaggtccaa cccttcctga
     5881 gtaaagaatc cgcgctcctg gccgtagtaa agatgagcgt aactgagcgt cggcccggga
     5941 atactgatgt aaaaccgctc cgccgccgat gcgctctggc tgacgccaac tgccagagcg
     6001 aggaacagcc caacagtgca acagcgaatt tgccgcatac cgcctcccga atttggtttg
     6061 ttctggaaat aaatcatttc gccgccagcg cagcataggg tccagccgca taaacaattc
     6121 gcccgccgac catcgtcagc aacgatacaa cgtttcgaat ctgctcctca ggtacgctta
     6181 agtaatcggc ggtgagcaca gccaggtcgg cgaacttgcc gacttcgatg gaacctttgc
     6241 gctcctcgtc agcagtcgtc caggcgctgc ccatggtgta catccgtaga gcttcggcgc
     6301 ggctgacatt ttgactacct tcgcgaatcg ccgatcccgc caccgtctta ccagtgatca
     6361 accaccacaa tgacagcatc ggcgcgtaac tcgccgaacg aaacgcgtcg gttccggcgc
     6421 cgactggaac gccgctatcg agcatcgtgc gtaggggcgg cgcgcggcgc gccttcgctt
     6481 caccccataa ctcgacgttg cgttcggccg tgagggccaa gcggtcctgc acggcgacgc
     6541 cgccgccgag attcttgatg cgcgcaaggg tttccgccgt cgcgtcttcg agatgggcaa
     6601 aaatcatgcg ctggggcgcc ggcggcgcgg ccgtaaacac ctgctcgatc actgttaaca
     6661 actggcgcgc cgtattgtcc tgggtcgcat gtaaatgaaa gctatgcccg gtctcggcca
     6721 gatagcgcat cactcggcga aatttctcct tcacttcagg cgaaagctgc accccttgcg
     6781 gattggacaa gacatcgccg tcgccgctgc cgcgcaccaa cgtttcagcg aatccagcga
     6841 agcgaaacag ctcatgttgg ccgaggcttt taatctcctc gacagccaat cgcaattgct
     6901 ccaactcgtc gccggactca gtcggcgcga tataatagtt gaagcgcaaa ctgagatcgt
     6961 cggtgcgcgc catgtcgaag agcagacgac gatgggcgaa tgtcacgccg ccggtgtgca
     7021 gatcgctcac cgaggtgatg cccaaacgat tcaattcatg aaagcaattg cgcaagcttt
     7081 gccgcgcttg cgccaacgta agccgcggga ttttattata ggctagttgc caagccgcca
     7141 cgccttgcag ccatccggtg gcaatcccgg tggcgggatc cttatcgatt cggccgccag
     7201 ggggatcggc agttttgctg gtgatgccaa gggtggcaaa cgccttggta ttgagcaacg
     7261 caccttgacg cagatattga atgtagacgg gatggttcgg cgcgatggcg tccagctcgg
     7321 cacgcgtcgg gaatcgtctc tcggccaact gcgtcggcac ccaaccgccg ccgaccacga
     7381 tccagcttcc cggcgcttgg ctctttgtgg ccgtcgcgat ttgccgcaag ccgtcggcga
     7441 gagtgcggtg aagttgccaa tgcagctcgg cgtcccagct caaccccgcc acggtggcgt
     7501 ggatatgcga atcgtttaat cccgggatcg ccgtccgtcc ggccagatcg atgacctgcg
     7561 tgtgggcgcc gatgtagtca cgcatctcgc tgtcgctacc aacggcgacg aagcggccat
     7621 ctctgatcgc caccgcttgt ttgatcgaga aagcgccgtc aacggtgacg atcttgccac
     7681 cgcgcaagac agtgtcagcg gcagacttga ccgccagcga acgagcgaag tcagcttcgc
     7741 gcccagagca gcccagcgaa accaatagca ctgcgatgag attcgatttc aacgttgatt
     7801 tcacgaactc tcccagacgg gcctgattct accctcggac cagccgattt gcgagccaag
     7861 atcgcgccat tgatcgatcc cgtcggaagc aatcgggccg gctttatagt tgcgttgcgt
     7921 caatatttgc agctatagtc cggaaaaatt tccaatccaa tacgctcgct agaggtgaac
     7981 gatgcaacct gtccaattgt tctgctggca tttcatggcc tatccctatc tcgacaaaga
     8041 cttcgacaag tctggacata gcggctgggt gaccaccccc aacaaactct gggacaacaa
     8101 ccaggctcgc ggcctctatc aggagtatat cgatcagctc gccctcgccg acgaattggg
     8161 cttcgacggc atggtgttaa acgaacatca tcaaaatatt tacgggttga tgccatcgcc
     8221 caacattatc gcctcagcgc tcacccagcg ctcctcgcgc ggcaagatcg t
//
LOCUS       DASQKS010000507         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5312385, whole genome shotgun sequence.
ACCESSION   DASQKS010000507 DASQKS010000000
VERSION     DASQKS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5312385"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VE170_13085"
     CDS             complement(<1..191)
                     /locus_tag="VE170_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16421.1"
                     /translation="MADSINRIVPPSGSVDRGRPVGRERAKEEPHEQKRKPPPDGAAA
                     PENQSEEPGGESDGAKDKG"
     gene            complement(252..1082)
                     /locus_tag="VE170_13090"
     CDS             complement(252..1082)
                     /locus_tag="VE170_13090"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HYJ16422.1"
                     /translation="MMKANKPSLAPSSSHAEPDPAKESIITKYLPLVRFVAEKVHRRL
                     PPGVDLESLVHSGVIGLLEALDRFDPKRGVEFEVYARYRIQGEVMQCLRSLDWVSRSV
                     RSWGRKMESARVKLAGQLVREPTADEMAKELAIPLDNYYHLDQQLNDATLLSLEDLSV
                     GSANEREGSRRGSIQNHDFDPLTLVESKDLIDKLGSAVKDLPERERLVITLYYHEELT
                     LKEIGAILNLSEGRICQIFAQTVGRLRTALGIAVKPKPGGKRGVETRKVSSAAKPVRV
                     "
     gene            complement(1079..1969)
                     /locus_tag="VE170_13095"
     CDS             complement(1079..1969)
                     /locus_tag="VE170_13095"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HYJ16423.1"
                     /translation="MIESVAFDKAALCSAPSPRSARAKRLTPAPVFAVTSGKGGVGKT
                     NVVANLATALARTNRRVMAIDADLGLANLDLFLGVKPAYTLADFFSGAAALDEIVVAN
                     RDGIMLLPGASGVAAITTLNRDQKNSLLTELDVMTEDLDLVLVDTGSGISDVVTYFTS
                     AAQEIVVVVTPELASITDAYALIKVLVSVHGHKRFAILANNVADQAQAWRLFDTLSGT
                     ALRFLNASLDFLGWIPRDPQLLRAMAQARTVVTEAPDAPSSRAFATLAEQFIRIAGRS
                     KVKGNMQFFLRRMLAAEIVS"
     gene            complement(1962..3008)
                     /locus_tag="VE170_13100"
     CDS             complement(1962..3008)
                     /locus_tag="VE170_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16424.1"
                     /translation="MNPKRFWGRDMGEALRAVRSSLGADALISDTRNVPKDQGGGVEI
                     TALADGPVAPDDSVVEPLVGELPNPAYPIAELRHELAGLKSMLAWLAPGLNHQDQIVN
                     TLLAHGLSIELIGKLNGTMQQMAGGDPRERWYRAIAQLIATGGEIAAGRDRLALVGPT
                     GAGKTSSLIKLTVVALQDEKRSVGWISMDQRSLAGSDPLAGYAAIFGVPYQRAVDRKT
                     LKEALDHLSDCVLVLIDTPGINPRDTASVKALAKQLHGLAEVRRTLVFNAVTPDKDLA
                     DWTARLRPVGAESLLFTKLDECRYFGPVVNTALNTALPVSYLSLGQNLSGDLVVARPQ
                     VFASLLLTGVGTDD"
     gene            complement(2998..>3279)
                     /locus_tag="VE170_13105"
     CDS             complement(2998..>3279)
                     /locus_tag="VE170_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HYJ16425.1"
                     /translation="DAHIERMIRERHQEGLSLEPQAAQRIMTAVQRSVESFTKRGQLP
                     VFLAGANVRRHLRQLISHYMPQVAVLSHNEIAAGVKIQSLGIIRWGDES"
BASE COUNT          655 a         1006 c          942 g          676 t
ORIGIN      
        1 ttccctttgt ccttggcgcc atccgactca ccgccaggtt cttcactttg attctccggc
       61 gcggcagcgc cgtccggagg gggcttgcgt ttctgctcat gcggttcttc ttttgctctt
      121 tccctgccaa ccggccgccc acgatcgacg gaacccgaag gaggaacgat tcgattaatc
      181 gaatctgcca tggccgcctt ttaaaaaaat tgcgactttc taagccgttt tcacggtttg
      241 ctcgtccatg tttaaacgcg aaccggtttc gccgcactcg atactttgcg cgtctcgacg
      301 ccgcgcttgc cgccgggttt gggctttaca gcaataccca gggcagtgcg caaacgcccc
      361 accgtttgag cgaagatttg acagatacgg ccctcgctca gattcaagat cgcgccgatt
      421 tccttaagcg tcagttcttc gtggtaataa agcgtgatca ccagccgctc gcgttccggc
      481 agatccttga ccgcgctgcc gagcttgtcg atgaggtcct tgctttccac caacgtcaac
      541 ggatcaaaat catgattctg gatgctgcca cgacggctgc cttcccgttc attggccgaa
      601 cccaccgaca gatcttccag gctgagcaat gtcgcgtcat tcaactgttg gtcgaggtga
      661 taatagttat ccagcggtat cgccaactct ttagccatct catccgcggt cggctcgcgc
      721 accaactggc cggccaactt gacccttgcg gattccattt tgcggcccca cgagcgcacc
      781 gagcggctca cccaatcaag agagcgcagg cattgcatca cttcaccctg aatgcggtag
      841 cgggcatata cttcgaattc aacgccgcgc ttgggatcaa accggtccag cgcctccaac
      901 agtccgatga cacctgagtg cacaagcgat tcgagatcaa ccccgggcgg caggcgccgg
      961 tgcaccttct cggcgacaaa acgcaccagg ggcagatact tggtgataat cgattccttc
     1021 gccggatccg gttcggcatg gctggaagac ggcgccaaac ttggcttatt cgctttcatc
     1081 atgagacgat ctccgcggcg agcatgcggc gtaggaaaaa ttgcatgtta cctttgacct
     1141 tgctgcggcc ggcgatccgg ataaattgct cggccagcgt ggcgaaggcg cggctcgatg
     1201 gcgcatccgg tgcctcagtc accaccgtgc gcgcctgcgc catagcgcgc agcaactgcg
     1261 ggtcgcgcgg aatccagccg agaaagtcga gcgaggcgtt gaggaatcgc aacgccgtcc
     1321 cggacagcgt atcgaacaag cgccatgcct gggcttgatc agcgacattg ttggcgagga
     1381 tcgcgaaacg tttgtgccca tgcactgaga ccaacacctt gatcaaagcg taggcgtcgg
     1441 tgattgaggc caactcggga gtgacgacca cgacgatttc ttgcgccgcg ctggtaaagt
     1501 aagttaccac gtctgaaata ccgctgcccg tatcgaccag caccagatcg aggtcttcgg
     1561 tcatcacatc caattcggtc aacagggagt ttttttgatc gcggttgaga gtcgtgattg
     1621 ccgccacgcc gctggcgccg gggaggagca tgatgccgtc gcggttggcg acgacgattt
     1681 cgtcgagagc cgccgcgccg gaaaaaaagt ccgccagggt gtacgccggt ttgacaccga
     1741 gaaataaatc caggttggcg aggccgaggt cggcgtcgat cgccatgacc cgtctgttgg
     1801 tgcgcgccag agcggtcgct agattggcga cgacattggt tttgccgacg ccgcccttgc
     1861 cgctggttac ggcaaacacc ggcgccggcg tcaaccgttt ggcacgggcg ctgcgcgggc
     1921 tcggcgcgct acagagcgct gccttatcaa acgctacgga ttcaatcatc ggttccgact
     1981 cccgtcagta gtaaactggc aaacacttgc ggccgcgcga ccacaaggtc gccgctgaga
     2041 ttctgtccta agctcaagta agaaaccggt agcgcggtat taagcgcagt gttcaccact
     2101 ggcccgaagt aacggcactc atcgagtttg gtgaaaagta gcgactcggc gcccaccgga
     2161 cgcaagcgcg ccgtccaatc agcgagatcc ttgtcagggg tcacggcgtt aaacactaac
     2221 gtccggcgca cctcggccaa accatgcaac tgtttggcaa gcgcttttac gctcgccgta
     2281 tcgcgcggat tgataccggg ggtatcgatc aacacgagca cacaatcgct caagtgatcg
     2341 agcgcctcct tgagggtttt gcgatccacc gcgcgctgat acggcacgcc gaaaatggcg
     2401 gcatagccgg cgagcggatc gctgccggcg aggctgcgtt ggtccatgct gatccagccc
     2461 accgaacgtt tttcatcctg caatgccaca actgtaagtt tgatcagact cgaggtttta
     2521 ccggcgcctg tggggccgac cagagccaag cggtcgcgtc cagcggcgat ttcgccgccg
     2581 gtggcgatca attgggcaat cgcccgatac cagcgttcgc gcgggtcgcc gccagccatc
     2641 tgttgcatgg tcccgttcag tttgccgatc agttctatgc tcaagccatg ggctaacaaa
     2701 gtgttgacga tctggtcctg atgattgagt cctggcgcca gccaggccag cattgatttc
     2761 aagccggcaa gctcatggcg cagctccgcg atcggataag ccgggttggg cagctcgcca
     2821 accaacggct cgacgacgct gtcgtccggt gccaccggtc catcggccag cgcggtgatc
     2881 tcgacaccgc cgccttggtc tttgggaaca tttctggtgt cgctgatcag cgcgtcggcg
     2941 ccgagcgaac tgcgcacggc gcgcaacgcc tcacccatgt cgcggcccca aaatcgttta
     3001 ggattcatcg ccccacctga taatgcccaa ggactgaatc ttgacgccgg cggcgatttc
     3061 gttatgcgac aagaccgcga cctgaggcat gtaatggctg atcaattggc gcaaatgacg
     3121 gcgcacgttg gcgcctgcca gaaacaccgg caactgaccg cgcttggtaa aactttccac
     3181 cgagcgctgc accgcggtca tgatgcgctg ggccgcctgg ggttcgaggg agagcccttc
     3241 ttggtggcgc tcacgaatca tccgctcgat atgcgcatc
//
LOCUS       DASQKS010000508         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2809573, whole genome shotgun sequence.
ACCESSION   DASQKS010000508 DASQKS010000000
VERSION     DASQKS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2809573"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VE170_13110"
     CDS             complement(<1..1096)
                     /locus_tag="VE170_13110"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16426.1"
                     /translation="MPKSLSTFLDECRREIPNEVVHIGKTIDPAHYDATAIIKQLGAQ
                     KKFPIAIFDKPLNLHGQVSDFPLVLNCEISQRKTQIALGMQRSLNRSEMAEACLAREA
                     KPIPPIVIDSKDAPVKQNIKRGADVDLFQLPFMRHYEMDGGPYSTLSTIARDRRSGIY
                     NCSYHRMEIKTKNSTALYASPRHLWRMYRDCEEQNMECPVATVMGHHPAYHIGACYTG
                     PFEVSEFDIIGAYLQEPLRLTPSETWGDDLLIPADAEIVIEGALIPGKRVVEGPFGEA
                     PGYLGPQRYVRCVEYEVRAINYRKGAMYQSVITPEGDKPWMDLPREGAYLRRCREAVP
                     GVTAVCKLGRQANYNIFIAMKKMSEGDPGRA"
     gene            complement(1322..2332)
                     /locus_tag="VE170_13115"
     CDS             complement(1322..2332)
                     /locus_tag="VE170_13115"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16427.1"
                     /translation="MANKGRDFVVVALVLGLQLYLAASGAAQELKKVRFGYPSLGFRQ
                     GHIWVAKDEGLFKKYGLDVEPIFLRGGQMAIQALASGDPPLMSIGQVVQASLAGHDLV
                     LIGGVETYYDSTVFARPEITRLEQLKGKRIGISGYGAATHFATIILANHLKIDPDKDW
                     QLIPGGPDAERIAAMSANKIDAGVFNSSTLPIAKRMGLVELVRIPDLKVEVQGNGMAT
                     TRAYIKSNRDVVKSALKGYIEGIHSIFSNKTGALKVFGKYMRTNDAEVLETSYQAYVT
                     TTPKKPYPTLKGLQFLLDRLAPQMPQAKTAKPEQFVDLSFLQELDKEGFFNEMAKRYP
                     AK"
BASE COUNT          477 a          724 c          689 g          544 t
ORIGIN      
        1 cggcccggcc cggatcgcct tcggacattt ttttcatggc aatgaaaata ttgtagttcg
       61 cctggcggcc gagcttgcac acagccgtga cgcccggcac cgcctcgcgg cagcgccgca
      121 agtacgcgcc ctcgcgcggc agatccatcc acggcttgtc gccctccggc gtaatgaccg
      181 actggtacat ggcgcccttg cgataattaa tcgcccgcac ttcgtactcg acacaacgga
      241 catagcgctg cggcccaaga tatccgggcg cttcgccgaa cggcccttcg accacccgct
      301 tgccgggaat caacgcgcct tcgatgacaa tctcggcatc ggcgggaatc agcaagtcgt
      361 cgccccaggt ctccgatggg gtcagccgca gcggctcttg cagataggcg ccgatgatat
      421 cgaactcgct gacctcgaac ggcccggtgt agcaagcgcc gatatgatag gccggatggt
      481 ggcccatcac cgtggccacc ggacattcca tattctgctc ttcgcagtcg cggtacatgc
      541 gccagagatg gcgcggcgaa gcgtacagcg cggtactgtt cttcgttttg atttccatgc
      601 ggtgatacga gcagttgtaa atgccgctcc ggcggtccct ggcgatggtc gacagcgtgc
      661 tgtagggacc gccgtccatc tcgtaatggc gcatgaacgg cagctggaag agatcgacat
      721 cggcgccgcg cttgatgttc tgcttcaccg gcgcgtcttt actgtcgatg acgatcggcg
      781 gtatcggctt ggcttccctc gccaggcacg cttcagccat ctcgctgcgg ttcaagctgc
      841 gctgcatgcc gagtgcgatc tgggtcttgc gctgggaaat ctcgcaattg agcacaagcg
      901 ggaagtcgct cacctggccg tgcaaattga gcggcttgtc gaagatcgcg atgggaaact
      961 ttttctgcgc gccaagctgc ttgatgatcg ccgtcgcgtc ataatgcgcc gggtcgatgg
     1021 tcttgccgat gtgaacgact tcgttcggaa tctcgcggcg gcactcgtcg agaaaggtgc
     1081 tcaagctctt cggcatgggg tctccgggtc aagaaacaaa ttcgcagtta ccgcacagac
     1141 gctgagcaag agggcaggtt tcaaatccgc ccccgcgatt cccgatactt attgcgccct
     1201 gtgcgttctt tgcggccatt ccttttttcg gttgcggctc taacacgcta tcgcttttgc
     1261 ggcagaactc gtcttgctgg agagggaacg gtgcgttctc tgcggctaga tatccgatct
     1321 tctacttcgc cggatagcgc ttggccattt cgttgaagaa accttcctta tccagctcct
     1381 gcaagaaact aaggtcgacg aactgttccg gcttggcggt tttggcctgg ggcatctgcg
     1441 gcgcgagacg gtcgagcagg aactgcaaac ccttcaaggt tggatacggc ttcttcggcg
     1501 tcgtggtgac gtaagcttgg taagaagtct ccagcacctc ggcgtcgttg gtgcgcatgt
     1561 atttgccgaa gactttgagc gcgccggttt tgttcgaaaa gatcgagtga atgccttcga
     1621 tgtatccttt gagcgccgac ttgacgacgt cgcggttgct cttgatataa gcgcgcgtgg
     1681 tcgccatgcc gttgccttgg acttcgactt taaggtcggg gattcgcacc aactcgacca
     1741 gtcccatgcg cttggcgatg ggcaaagtcg aagagttaaa gaccccggca tcgatcttat
     1801 tcgccgacat ggcggcgata cgctccgcgt cgggaccgcc gggaataagc tgccaatcct
     1861 tgtccgggtc gatcttgaga tgattcgcca gaatgatcgt agcaaaatgg gtcgccgcgc
     1921 cgtaaccgct gatgccgatg cgtttcccct tcagctgttc cagacgggta atttccgggc
     1981 gtgcgaaaac ggtcgagtcg tagtaagtct ccaccccgcc gatcagcacc agatcatggc
     2041 cggccaatga cgcttggacc acctggccga tgctcatgag cggcggatcg ccgctggcca
     2101 gggcttgaat cgccatctgc ccgccgcgca gaaaaattgg ttcgacatcg aggccgtact
     2161 ttttgaatag tccttcatcc ttcgccaccc agatatgacc ctggcgaaat cccagagagg
     2221 ggtaaccgaa gcgaactttt ttcagctcct gagccgcgcc gcttgccgcc aaatacaatt
     2281 gcagcccgag aaccaaagca actacgacaa aatcacgtcc cttgttcgcc atcaccggct
     2341 cctaggcaat ttacctgggg tacaactgga tttgcattcc ctcaggccaa gccgaaccac
     2401 tcatcaaatt ctattgtcca aaaatttccc ggaa
//
LOCUS       DASQKS010000509         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4464594, whole genome shotgun sequence.
ACCESSION   DASQKS010000509 DASQKS010000000
VERSION     DASQKS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4464594"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VE170_13120"
     CDS             <1..693
                     /locus_tag="VE170_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYJ16428.1"
                     /translation="ANFMKIFRIASFIVGITALCGIASAGTIKGSVRYSGAALEKKKI
                     AVTIDQYLCGKEKTAGNLLLSPNNGIYNAVVSLQGVRAGAKWPANAAPVKMDQIKCDF
                     IPRVVIVPAGGTVEFLNSDRLMHNVRSGGKENPPFNRAQPHARTIAIGFKHPEVLRVD
                     CDLHSWMRGWVVVAEHPFYTVTNDAGEFGFENVPPGKYTLQVWQETLGTTTQEVTVSG
                     DSTSVAVRMQKR"
     gene            complement(706..1158)
                     /locus_tag="VE170_13125"
     CDS             complement(706..1158)
                     /locus_tag="VE170_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HYJ16429.1"
                     /translation="MKIISQRSAVIIALAGLALVTLLAGALTPPVWAGQAEQFTPEFH
                     GLHKNFDPKHTPKIVAPDSVKRGQWFDVTVSVGSGSEHPSLSEHFVRYIALYIDSAEI
                     SRVYLHPVYSFPKVTFTIALDRGGVLRALEEPTHSSAWEGSKKISVLP"
     gene            complement(1204..2265)
                     /locus_tag="VE170_13130"
     CDS             complement(1204..2265)
                     /locus_tag="VE170_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ16430.1"
                     /translation="MAIPTPKILAVDDNIQNLELLTRALSAAKYEVITAGDGASALQL
                     IDDGAVDLVLLDVMMPGMSGYEVCEKIRANDATRLLPVVMLTALHDVSHRIQGIAAGA
                     DDFLTKPFNREELLTRVKSLLRIKTLYDDIETKNVLLRRVIGRYVSEEVAAEIVADPG
                     RHLKLGGEKRDVTVVFGDLRGFTPLAERLDPEDVVEILNVYLSHVVDLVFEFRGNLDK
                     FRGDGFMAFFGAPLAHGDDAASAVRCGLALQGRLNSISFAKFPDTRLSVGIGINSGIV
                     IAGNVGSERRADYTVIGNEVNLAQRFEANAGPGQILITGATYERVKESVEVRELGRLR
                     VAGMQEGVMAYDVLRWRAA"
     gene            complement(2286..>2525)
                     /locus_tag="VE170_13135"
     CDS             complement(2286..>2525)
                     /locus_tag="VE170_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ16431.1"
                     /translation="ELPDLILMDVQLPKLSGLDATRQLRADAKTAAIPIVVITSFALS
                     GDAEKAKAAGASAYLAKPYSPRELLQMIRNLVPEN"
BASE COUNT          565 a          733 c          716 g          511 t
ORIGIN      
        1 gcaaatttta tgaaaatttt tcgcatcgct tcgtttattg tcgggatcac agcgctttgc
       61 gggatagcca gcgccggcac gatcaagggc agcgtgcgct acagcggcgc agccctggaa
      121 aaaaagaaaa tagcagtaac catcgaccaa tatctctgcg gcaaagagaa aacagctggc
      181 aacttgctgc tgtcgcccaa caacggcatt tacaacgccg tcgtatcgtt gcagggggta
      241 cgggccggcg caaagtggcc tgctaacgcg gccccggtga aaatggatca gatcaagtgc
      301 gacttcattc cccgcgtagt catcgtgccg gcgggcggca ccgtggaatt tttgaacagt
      361 gaccggctga tgcacaacgt ccggagcggc ggcaaagaaa atccgccgtt taatcgggcg
      421 caaccgcacg cgcgcacgat tgccatcggc ttcaaacatc ccgaggtact gcgcgttgac
      481 tgcgacctac actcatggat gcgcggctgg gtagtggttg ccgaacatcc cttttacaca
      541 gtcactaatg acgcgggtga atttggtttt gagaacgtac cgccgggcaa gtacacgctg
      601 caggtttggc aggaaacctt ggggaccacg actcaggaag tcacggtgag tggcgacagc
      661 accagcgtcg ctgtgcgcat gcaaaagcga taattttaaa ttcagctaag gcagcacgga
      721 tattttcttt gagccttccc acgccgacga atgggtcggc tcctccaaag cgcgcagcac
      781 gccaccgcga tccagggcga tggtgaatgt gaccttcggg aacgaataca ccggatgaag
      841 atagacgcgg ctgatctccg cgctatcgat gtataaggcg atatagcgca cgaagtgctc
      901 gctcaacgac ggatgctcac tgccgctgcc gaccgaaacc gtaacatcga accattgacc
      961 gcgcttgacg ctgtcaggcg cgacgatttt cggcgtgtgt ttggggtcaa agttcttgtg
     1021 caggccgtga aactcgggcg taaactgctc ggcctgcccg gcccaaaccg gcggcgtaag
     1081 cgcgccggca agcagtgtaa cgagcgctag cccagctagc gcgatgatca ctgctgatcg
     1141 ctgagaaatt attttcatag ttttctatta ctcctgaaac tactttcgtt gttgccaaat
     1201 caattacgcc gcgcgccaac gcagcacgtc gtaagccatc accccctctt gcatgccggc
     1261 cacacgcaag cgaccaagct cgcgcacctc gacagattcc ttgacacgct cgtaggtggc
     1321 gccggtgatt agaatttggc caggcccggc gttggcttcg aagcgttgcg ccagattgac
     1381 ctcattgccg attaccgtgt agtccgcgcg ccgttccgag ccgacgttgc cggctatcac
     1441 aatgccgctg ttgatgccga tgcccaccga cagacgggtg tcgggaaact tcgcgaagct
     1501 aatgctattg agccgcccct gcaaagcaag tccgcagcgt accgcgctcg cggcatcgtc
     1561 gccatgagct agcggtgcgc cgaaaaacgc catgaagccg tcgccgcgaa acttgtcaag
     1621 gttgccgcga aactcaaaga cgagatcaac aacatggctc aggtagacat tgagaatctc
     1681 gaccacgtcc tcgggatcga gccgttccgc gaggggcgtg aagccgcgca gatcaccaaa
     1741 caccacggta acatcgcgct tctcgccgcc caattttaaa tgccgtccag gatccgcgac
     1801 gatttcggcg gcgacctctt ctgaaacgta gcggccgatc actcggcgca gcaacacatt
     1861 cttggtttcg atgtcgtcat aaagtgtttt aatccggagc aaagatttca cccgcgtcag
     1921 cagctcctcg cggttaaacg gcttagtcaa aaaatcgtcg gcgccggcgg cgatgccctg
     1981 aatccggtgc gacacgtcat gcagggcggt gagcatgacg accggcaaga gccgcgtcgc
     2041 atcgttggcg cgaatctttt cgcaaacctc gtaccccgac attcctggca tcatgacgtc
     2101 gagcagcacc aaatccaccg cgccgtcgtc aatcagctgc aatgcgctcg ccccgtcacc
     2161 ggcggtaatc acttcgtact tggccgcgct caacgctcgg gtcagcagtt cgagattctg
     2221 gatattgtca tcgacggcga gaatttttgg agttggaatg gccatggctt tctttgcaac
     2281 ggcagtcaat tttccgggac gagatttcga atcatttgca acagttcgcg cgggctatag
     2341 ggcttggcca gataggccga tgcgccggcg gccttggcct tctcggcatc gccactgagc
     2401 gcgaaggaag tgatgacgac aatcggtatc gctgcggttt tagcatcggc gcgcagctgg
     2461 cgcgtcgcgt cgagaccgga cagcttgggc aattggacgt ccatcagaat gagatcgggc
     2521 aattc
//
LOCUS       DASQKS010000510         4112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4465278, whole genome shotgun sequence.
ACCESSION   DASQKS010000510 DASQKS010000000
VERSION     DASQKS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4465278"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VE170_13140"
     CDS             complement(<1..917)
                     /locus_tag="VE170_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16432.1"
                     /translation="MKKLILCALTVSLAAALLSMPCAGQTLKVGMTSKTLFYLPFYVG
                     QKRGFYNQENIKVELILIGRTDVHLQALLSGEINFGTLNADSVIAVGEKGAILKVVAG
                     VDNAAPYFLIGGKNYKKVEDLKGARLGVSSLRGGATSILLEYLKTKGLLFPRDFILTV
                     ISGGTVSRLTALETGAIAGAVLGFPYSDIAVDQGFTRLGDTMDVISTYQFNGLTVNPS
                     WAEKNRANVVRFLKAHIRSLRWIHEHPDQAADLFTAEMGIKPPYARRGVDYFTKNKVF
                     PIDGSVTIDGLKANIDIQFKDGILKEPLPT"
     gene            1054..2082
                     /locus_tag="VE170_13145"
     CDS             1054..2082
                     /locus_tag="VE170_13145"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16433.1"
                     /translation="MKCFARAMILLAFLVSHGAAYANPYLAKAGEAPFTARVATCAIT
                     GGFIHLYTAFDNGLFDKYGMKVEFVSIRGSGVALAALAADEIQFLYCAADATIPGMAA
                     GSDAKLIAAPLVGLPWVLLTRKDIKRPEELKGKSIAVTRPGDLTFRLARALLKKFNLT
                     ENDVRILTVGGTGQVEPFNAMRSGFAEAALVTPPLDVRGRRDGFSLIYRLNDLALPAV
                     YSSLHTNAKTLRERPQLVQKWVAALAESIQFVERNPDRGKAAVSKALKLNDGDALQSA
                     YDAYAKLLVNRRLTVPEGSVAGVIEVAREQGTNIRRKASEMIDNRFAEDLDKSGFLKE
                     IWSGELPR"
     gene            2099..3052
                     /locus_tag="VE170_13150"
     CDS             2099..3052
                     /locus_tag="VE170_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYJ16434.1"
                     /translation="MLRVVWSLIFCLLPHCIHAQGYPKQPINLIIPLAPGDATDVAGR
                     TIGEGISKLLKVPVVAVNRPGAGGTIGTDSAVKAPKDGYTILITNNASMVFNRVLTPE
                     AVPYDPYKDLTPLGMAARTPIVLAVHKDAPFKNFAEMIDYGKKNPGGIRIGTVGPGSV
                     GHFTVEIVNSISPADMTMIPFKGAGPAVTAALGGHIEGAAAAQGVVAEHLKAGTMRGI
                     IASSKMPEFPQIPTLAQLGYKQNLLGVWSAFFAPAGVSAEVSKTLIAALEKVIKDPGV
                     AARLGNLGMVADYESPEKLIAEIREEHRAVEQIAKKAGLIK"
     gene            complement(3137..3751)
                     /gene="ureG"
                     /locus_tag="VE170_13155"
     CDS             complement(3137..3751)
                     /gene="ureG"
                     /locus_tag="VE170_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HYJ16435.1"
                     /translation="MKKSQPLSVGIGGPVGSGKTALTLALCRALRERYDLAVVTNDIY
                     TEEDAQFLVRHQALPAERILGVETGGCPHTAIREDASINLEAVDRLCRRFPNLDLIFV
                     ESGGDNLAASFSPELADLTIYVIDVAAGDKIPRKGGPGITKSDLLIINKIDLAPLVGA
                     SLEVMDRDAKKMRGDKPFVFTNLKTGAGVADVIGFLEKQALATV"
     gene            complement(3759..>4112)
                     /locus_tag="VE170_13160"
     CDS             complement(3759..>4112)
                     /locus_tag="VE170_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HYJ16436.1"
                     /translation="NQIDGIDSDALTYMNNLVEIAYPAAFACVVVSWNLPPPAALSAY
                     LWSWAENQVMAALKIVPLGQTEGQRMLLSLGGRLSAIVERASALGDDEIGAFTPGLAI
                     LSSRHETQYSRLFRS"
BASE COUNT          874 a         1178 c         1204 g          856 t
ORIGIN      
        1 gtcggtagcg gttctttcag tatgccgtct ttgaactgga tatcgatatt ggctttcaat
       61 ccgtcaattg tcaccgagcc gtcgatggga aacactttgt tcttggtaaa ataatcgacg
      121 cctcgccgtg cgtagggcgg cttgataccc atttcagcgg tgaataagtc ggcggcttgg
      181 tccgggtgtt cgtggatcca gcgcagcgag cggatatgcg ccttcaagaa gcgcactacg
      241 ttggcgcgat tcttctcggc ccaggatggg ttcacggtga ggccgttgaa ttgatacgtc
      301 gagataacgt ccatggtgtc gcccaagcgg gtgaatccct ggtcgacggc aatatctgaa
      361 tagggaaaac ccaacaccgc cccggcgatg gcgccggttt ccaacgcggt caatcggctc
      421 acggtgccgc cggagatcac ggtcaagata aaatcccgtg gaaaaagcag tcccttggtc
      481 ttcagatact ccagcaagat cgaggtcgcg ccgccgcgca agctcgacac cccgagtcgc
      541 gcgcccttaa ggtcttcaac ttttttatag tttttgccgc cgatgagaaa atatggcgcg
      601 gcgttatcga caccggcgac gaccttcaga atcgcccctt tctcgccaac ggcgataacg
      661 ctgtcggcgt tgagggtgcc gaagttgatt tcgccactga gcagcgcctg gagatgaaca
      721 tcggtgcggc cgatgagaat caattcgacc ttgatatttt cttggttgta aaagccgcgt
      781 ttttggccga cgtaaaatgg caggtagaaa agcgtcttcg acgtcatgcc gactttaaga
      841 gtctgtccgg cgcagggcat ggagagcagc gctgccgcaa gcgagactgt tagggcgcac
      901 aaaattagtt ttttcaccgt aaaattctcc ttcgacaccg gcgcagggaa tgacggatac
      961 tacatggctc gatccatcga ttacaacaaa agaaatctga cttgacagcg accgccgacg
     1021 gatgtatgcg cagagtctca taaggaggca gcgatgaagt gtttcgcgcg cgcaatgatc
     1081 ctcctcgcct tcttggtctc gcacggtgcg gcctacgcca atccctattt ggcgaaggcc
     1141 ggggaagctc cgttcacggc gcgcgtcgct acgtgcgcga tcaccggcgg ctttattcat
     1201 ctatacaccg ctttcgacaa cggactcttc gataaatacg gcatgaaggt cgagttcgtt
     1261 tcgattcgcg gtagcggagt cgccttggcg gcgctggccg cagatgagat acagtttctc
     1321 tactgcgccg ccgacgcgac aattcccggc atggccgccg gcagcgacgc caagctgatc
     1381 gccgcgccgc tcgtcggact gccttgggtt ttactcacgc gcaaagacat caaacggccc
     1441 gaagagttga aaggcaaaag tatcgcagtg acacgccctg gcgatcttac ttttcgttta
     1501 gcgcgcgcgc tgctcaagaa attcaatctc acggaaaatg acgtgaggat tcttacggtc
     1561 ggcggcaccg gccaagtgga gccttttaat gccatgcgct ccggcttcgc cgaagccgcg
     1621 ctggtcacgc cgccgttgga tgtgcgcgga cggcgcgatg gtttcagctt gatctaccgg
     1681 ctcaacgatc tcgctttgcc ggcggtctat agttcactcc acaccaacgc caagactctt
     1741 cgggaacgcc cgcaactcgt gcaaaaatgg gtcgccgcat tggcggagtc gattcaattc
     1801 gtcgaaagaa atccagacag aggcaaggcc gccgtgagca aggcgctgaa gctcaacgat
     1861 ggcgacgccc tgcaatccgc ctacgacgcc tatgccaagc tgctggttaa ccgacgcctg
     1921 acggtaccgg aaggcagcgt cgccggcgtt atcgaggtcg cgcgcgagca gggaaccaat
     1981 attaggcgca aagccagtga gatgatcgac aaccggttcg ccgaggattt ggacaaaagc
     2041 ggttttctca aggaaatctg gagcggcgaa ctgccgcggt agatagagga gtggaatcat
     2101 gctgcgtgtc gtctggagtt tgattttttg tttattgcct cattgcatcc acgctcaggg
     2161 ctatccaaag cagccgatca acctgatcat tcccctcgcc ccgggcgatg ctacagacgt
     2221 cgccggccgc accatcggcg aaggaatatc caaactgctc aaagtcccgg tggtggcggt
     2281 gaaccggccc ggcgcgggcg gcaccatcgg caccgacagc gccgtgaagg cgcccaaaga
     2341 tggctacacc attctcatca ccaataacgc ctcgatggtg ttcaaccgtg tcctaacgcc
     2401 ggaggcggtg ccttacgatc cctataaaga tctgacaccg ctgggcatgg ccgcgcgcac
     2461 accgatcgtt ttggcggtgc ataaagacgc tccgttcaaa aacttcgccg agatgatcga
     2521 ttacggcaag aaaaatcccg gcggcattcg tatcggcacc gtcggtcccg gttcggtcgg
     2581 ccattttacc gttgagattg tcaattcgat cagcccggcc gatatgacga tgattccgtt
     2641 caaaggcgcc ggcccagcgg tgaccgccgc tttaggcggc catatcgagg gcgctgccgc
     2701 ggctcaaggc gtcgtcgccg agcatttgaa ggccggcacc atgcgcggca tcattgcatc
     2761 gagcaagatg ccagagttcc cgcagatccc gaccttggcg caactgggct ataagcaaaa
     2821 cctattgggc gtttggtcgg cgttcttcgc gcccgcaggc gtgtcggcag aagtgtcaaa
     2881 aacattgatc gccgcccttg agaaagtcat caaagatccc ggcgtggcag cgcgcctcgg
     2941 caacctcggc atggtcgcgg attatgagtc gcccgaaaaa ctgatagcgg aaatacgtga
     3001 agaacatcgc gccgtcgagc agatcgccaa gaaggccggc ttgatcaagt agggtgtcga
     3061 gttgttgctc gattggtgat ggataagcgt agtcacactg cacctaccgt actttcgcgg
     3121 gacttgaatt ttcccttcac actgtcgcca acgcctgctt ttccaaaaac ccaatcacgt
     3181 ccgcgactcc cgcgccggtt ttcagattgg tgaagacgaa tggtttgtcg ccgcgcatct
     3241 ttttggcgtc gcgatccatc acttcgaggg aggcgccgac cagtggcgcg agatcgattt
     3301 tgttaatgat caacagatcg ctcttggtga tgccggggcc gcccttacgg gggatcttgt
     3361 cgccggcggc gacgtcgatc acgtagatcg taagatcggc caattcggga ctgaagctcg
     3421 cggctaagtt atcgccgccc gactcgacaa atatcaggtc cagattgggg aatcggcggc
     3481 acagccggtc caccgcttcg aggttgatcg aggcgtcttc gcgaattgcg gtatgtgggc
     3541 agccgccggt ctcaacccca aggatgcgtt cggccggcag cgcctggtga cgcaccagga
     3601 actgggcatc ctcttcggta taaatatcgt tggtgaccac ggcgagatcg tagcgctcgc
     3661 gcagggcgcg gcagagcgcg agggtcagcg cggtcttgcc ggaaccgacc ggcccgccga
     3721 tgccgacgga gagaggttgc gattttttca tatgattcct aggagcggaa cagcctggaa
     3781 tattgcgtct catggcggct cgacagaatt gctaggcccg gcgtgaacgc accgatctcg
     3841 tcgtcgccca acgccgacgc ccgctcaacg atcgcgctta gccggccgcc cagggatagc
     3901 agcatccgtt ggccctcggt ttgtcctaga ggcacaatct ttagcgcggc catcacctga
     3961 ttctctgccc acgaccagag ataggccgaa agcgcagccg gcggcggcaa gttccatgag
     4021 accacgacgc acgcgaatgc ggcgggatag gcaatctcga ccaggttgtt catgtaggtc
     4081 agcgcgtctg agtcaatgcc gtctatttgg tt
//
LOCUS       DASQKS010000511         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3228753, whole genome shotgun sequence.
ACCESSION   DASQKS010000511 DASQKS010000000
VERSION     DASQKS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228753"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VE170_13165"
     CDS             complement(<1..310)
                     /locus_tag="VE170_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16437.1"
                     /translation="MADHSTTAESAANAGSETPTLEVTLKQKRLALGVISGCHVLNHL
                     QYNITSVMFPVMMKELGFGLLQLGALSAISNFVGQGLQVVYGLFTGMFKRTVMLGAGN
                     V"
     gene            complement(440..1978)
                     /locus_tag="VE170_13170"
     CDS             complement(440..1978)
                     /locus_tag="VE170_13170"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HYJ16438.1"
                     /translation="MNANRSAPAAKRNLAATGVMMVIFLFAIDATVVSTSMPTIVARL
                     GGLELYSWVFTIYMLTSALTTPIFGKLADLFSKRRLMLIGIAIFLIGSTLCGAAQSME
                     AMIVFRAVQGLGGGAIYSLSFIVIGVIFPAEQRAKMQGIISGIWGLASILGPLAGGVI
                     VENWSWRWIFFINLPLIAIATTLIVVGLKEEAGTRRAARLDIAGACTLTVGLLLLFYA
                     LAQSAHARYPLNLETLGYIGAGLVILIVFYYIERRAAEPILPVDLFHIGLYKSSAAVA
                     TISAMGVFGAISFMPLFLQGVIGLTATTTGLIILILSMGWTAGSLLAGRWINRFGYRF
                     AAVTGMALLAIGYAIFVAPQFSASIVAVGFSAGAIGVGMGMANLTTLVAAQSSVAPQR
                     IGVATSTIMLFRTFGSAFAVSVMGTVMLSKMQSSLGQLRAANPTVDAAVWAKLANPQN
                     LLDPLTRAQVSADMLPKLIASLGDALWYAFLTGFVLMLLGLAISFVMADYTPANTHRA
                     QPNS"
     gene            2118..3116
                     /locus_tag="VE170_13175"
     CDS             2118..3116
                     /locus_tag="VE170_13175"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16439.1"
                     /translation="MLQTLITFITISYFLVPRMAAAQITAELVEAAKKEGEVMLYGAI
                     TVNSSKAVGDAFEKKYGIKLRHWRGDATELINRSLAEARAGKSAFDVTLGNEVVMAAL
                     DEKSLFAVFEPPAAKAFPKQFLDPDKKMTPWRVLPYGINYNHQLLKAEEAPKTWEELL
                     QPKWKGKFGVANPGIHVTTLQFVLNLDKLLGPKWLKVVEGWAQQEPRVGRSLADNIQP
                     LTSGEIQVAVGYIKDKFQYPGPIEYVRMNKYLASLSFIAINRQAPHANAARLFADFFV
                     GPEAQRIFGNFGEYVFHPEADHKFKKDVKDDQILVMRLPNNEELTSWSKKFREMFR"
BASE COUNT          747 a          850 c          974 g          638 t
ORIGIN      
        1 cgacattgcc cgcgccgagc atcaccgtgc gcttgaacat gccggtgaac aagccgtaaa
       61 cgacctgcag gccttggccg acgaaattgg aaatagccga aagcgcgcct agttgcagca
      121 agccgaatcc caactccttc atcatcaccg gaaacattac cgaagtgatg ttgtattgga
      181 gatgattcaa gacgtggcag ccgctgatga ctccgagcgc caggcgcttt tgtttcagag
      241 tcacttcgag cgtcggtgtt tcactgccgg cgttggcggc ggattcagct gtcgtcgagt
      301 gatcggccat ggctctagat tccttgaaat gaagtgtcgc aataatcgat tactgcgttt
      361 ggtttatagc gccgcattgg tttgtgtcaa acgcgacgat tccgactcgg gtttggcggt
      421 cggagcgaag tactaatctt taggagtttg gctgcgcccg gtgtgtattt gccggcgtgt
      481 aatcggccat gacaaaactt atcgcgagac cgaggagcat gagcacgaag ccggttaaaa
      541 atgcatacca gagcgcgtcg cccagactgg cgattaactt gggcagcata tccgcagaaa
      601 cctgtgcgcg ggtgagcggg tccaagagat tttgcggatt ggccagtttt gcccagaccg
      661 cggcgtcgac ggtggggttg gcagcgcgca attggccgag agagctttgc atcttactga
      721 gcatcaccgt gcccatgacg ctaacggcaa acgccgagcc gaaggtgcgg aacaacatga
      781 tcgtcgaggt cgccacgccg atgcgctgcg gcgcgaccga actttgggct gcgaccagag
      841 tggtgagatt ggccataccc atgccgacgc cgatggcgcc ggcgctaaat ccgacagcga
      901 cgatgctggc gctaaactgc ggcgcgacga agatggcgta gccgattgcg agcaaagcca
      961 tgccggtcac cgcggcgaag cggtagccga accggttgat ccatcggccg gcaagcaaac
     1021 tgccggcggt ccaacccatg cttagaatca aaataatcag accggtggtc gtggcggtca
     1081 ggccaattac gccctggagg aacagcggca tgaagctgat cgcgccgaac acgcccatgg
     1141 ccgagatcgt ggcgacggcg gcggacgatt tgtacaagcc gatgtgaaac agatcgacgg
     1201 gcaatatcgg ctccgcggcg cggcgctcga tgtaatagaa aacgattaaa atcaccaagc
     1261 cagcgccgat gtagcccaag gtttccaggt tcagcgggta gcgcgcgtga gcactctgcg
     1321 ccagagcgta gaacaataac agcaagccaa cggtcaaggt gcatgccccg gcgatatcga
     1381 gtcgagcggc gcggcgcgtg cctgcttctt ctttcaagcc gacgacgata agtgtcgtgg
     1441 cgatggcgat cagcggcagg ttgatgaaga agatccaacg ccaactccaa ttctcgacaa
     1501 tgacgccgcc ggccaggggg ccgaggatgg aggcgaggcc ccagatgccg ctgatgatgc
     1561 cttgcatctt ggcgcgctgc tcggcgggaa agatcactcc aatgacgata aaggacagcg
     1621 aatagatggc gccgccaccg agcccttgca cggcgcgaaa gacgatcatc gcctccatgc
     1681 tctgggccgc gccgcataat gttgagccga tgagaaatat cgcgatgccg atcagcatca
     1741 gccggcgttt gctgaacagg tcggcgagct taccgaagat cggcgtggtc agcgccgagg
     1801 tcagcatgta aatcgtaaat acccagctat agagttccag gccgcccaac ctggcgacga
     1861 tggtcggcat cgacgtgctg acgacggttg cgtcgatggc aaaaagaaaa atcaccatca
     1921 tgacgccagt ggcggcgaga ttgcgcttgg cggcgggggc gcttcggttg gcattcatga
     1981 cggcggcgat aaacgatgta agttctcttg agttgtctta gattggcttg ggagtcaagc
     2041 cgcggcgaaa gttgcgcatc gcgttgacgc gcgctcccgg cagcgctaaa agagcagcta
     2101 tcttacggag gcacctgatg ttgcagacgc taatcacttt catcacgatc agttatttcc
     2161 ttgttccgcg catggcagcg gcgcagatca ccgctgaatt agtcgaagct gccaaaaaag
     2221 agggcgaggt gatgctctac ggcgctatca ccgttaactc gtcgaaggcg gtcggcgatg
     2281 cctttgagaa gaagtacggc atcaagctgc gccactggcg cggcgatgcg accgaactca
     2341 tcaatcgcag tcttgctgaa gcgcgggcgg gtaagtcggc gttcgacgtc accctgggta
     2401 acgaagtggt gatggccgcg ctcgatgaga agagcctgtt tgccgtcttt gagccgcctg
     2461 cggcaaaggc ctttccgaag caatttcttg acccggacaa aaagatgacg ccctggcgtg
     2521 tgttgcccta cggcatcaat tacaatcatc aactcttgaa ggctgaagaa gcgcctaaaa
     2581 cttgggagga gctgttgcaa ccgaagtgga aagggaagtt cggcgtcgcc aatccgggca
     2641 ttcacgtgac aacgctgcag ttcgttttga atctcgacaa gttgttgggt cccaaatggc
     2701 ttaaggtggt cgagggatgg gctcaacagg agccgcgggt agggcgcagt ctagcggaca
     2761 atatccagcc gttaacctcc ggcgagatcc aggtcgccgt cggctatatc aaagataagt
     2821 ttcagtatcc cggtcccatc gagtatgtgc gcatgaataa atatttggcg tcattgagtt
     2881 tcatcgcgat caatcgccag gcgccacacg ccaacgcggc gcgcctgttc gctgatttct
     2941 tcgtcgggcc tgaagcccag cgcatcttcg gcaacttcgg cgaatatgtg tttcatcccg
     3001 aagccgatca taagttcaaa aaagatgtga aggatgacca gatcctagtc atgcggctgc
     3061 caaacaacga ggagctgaca agttggagca agaagtttcg ggaaatgttt cggtaacgta
     3121 aattggagta atggagtggt ggagtaatgg agtaatgggt gtcggaccca tcattccacc
     3181 actccgatac tccatcattc cattggcca
//
LOCUS       DASQKS010000512         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_706385, whole genome shotgun sequence.
ACCESSION   DASQKS010000512 DASQKS010000000
VERSION     DASQKS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706385"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VE170_13180"
     CDS             complement(<1..766)
                     /locus_tag="VE170_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16440.1"
                     /translation="MAQQVTPWQVLKQRDFGLFWASLLFSAVGSQISTVAIAWQVYEI
                     TNSPFQLGLAGLFRALPIMILSIPGGVLADRMDRRHLLIITQSLAALLALALALLSYF
                     AAVEVWQIYLVTFLSGAVGIFDAPARTALIPALVRPEQLATAYALNITWRQIATLSGP
                     FIGGVIIATVGLSASYFIDALSFLSVIVCLGLMRARPAAASGEKKESPLESVRSGFNF
                     IRDNSAILGLMGMDTCVQFFGAYRSMMPVFARDILAV"
     gene            908..1918
                     /locus_tag="VE170_13185"
     CDS             908..1918
                     /locus_tag="VE170_13185"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16441.1"
                     /translation="MKRLAFLGFLTVTLFSSVTAWAQKPLPTVRIGIEAPAGTNSHYH
                     VTKQAGLFQKYGLNMELISFPGGTVGMQSLIAGDIQFATQDGVATLTANLRGANIYFV
                     GGMINTFPFLILSRPEIKVPADLRGKKIVISRYGSSSDTAARAAVEKYGLKPDKDVII
                     LQGGGQSERFAALKAGVVDAAIVSPPLNLAGRQLGFNEVIDLSDSGVPYAHQLIVATK
                     DFLDRNPDLTMRTLRALIEGLGLWKDPAKKPQVLKSIGHYLRLDPEKQKDQLEETYRY
                     YSKTFPTKPYPTLEGVEFTAQILKRNRPEAKDIQAKDYVLYKYVAELEKEGFLTKVFG
                     GK"
     gene            complement(1982..2131)
                     /locus_tag="VE170_13190"
     CDS             complement(1982..2131)
                     /locus_tag="VE170_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16442.1"
                     /translation="MPLHVKSQDVPIRKGVRTGVEGEGSMISVKGYGNECSLMIATRA
                     PGAKE"
     gene            complement(2201..>2625)
                     /locus_tag="VE170_13195"
     CDS             complement(2201..>2625)
                     /locus_tag="VE170_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16443.1"
                     /translation="SVDTTTVGGNADMFSIMRLMLQLNHLRSMDVMEVQPRRILELAT
                     LDGAKDLGIADRTGSLTPGKRADLILVRTNDINVAPFINPALLLVQQANPFNVDTVVI
                     DGRILKHKGELVAIDTEEVINKAAEAFHAARKRAGGPY"
BASE COUNT          586 a          747 c          758 g          534 t
ORIGIN      
        1 cgacggcgag aatgtcgcgg gcaaacaccg gcatcatcga acgataagcg ccaaaaaact
       61 gcacgcaggt gtccatgccc atcagcccga ggatcgccga gttgtcgcgg atgaaattaa
      121 aaccgctgcg cacgctttcg aggggcgatt ctttcttttc gcccgatgcg gcggctggtc
      181 ttgcgcgcat cagcccgagg cagacgatca cgctcaaaaa actcagcgcg tcgataaagt
      241 aggaagcgct gagaccgacg gtggcgatga tgacgccgcc gatgaacgga ccggagagcg
      301 tggcgatctg gcgccacgtg atgttgagcg cgtaggcggt cgccagttgc tccggccgta
      361 ccagtgccgg gatcagcgct gtgcgcgccg gtgcatcgaa aatccccacg gcccccgaaa
      421 gaaacgtgac gagatagatc tgccagacct ccacggcggc gaaataactc aacagcgcca
      481 gcgccaacgc cagcagcgcc gccaagcttt gagtgatgat caacaggtgg cgccggtcca
      541 tgcggtccgc gagcacgccg ccaggaatcg ataggatcat gatcggtagc gcgcgaaaga
      601 gcccggctag acccaattga aacggtgagt tggtgatctc gtaaacttgc caggcgatgg
      661 cgaccgtgga gatttggctg cccaccgcgg aaaaaagcag gctcgcccag aacagaccga
      721 agtcgcgttg tttgagaacc tgccagggtg tgacttgctg cgccatgaaa aatcgatctc
      781 tttgactgtg gtcgccaacg accgtagagg aatcttacgc atcgagcaat cgacgatcca
      841 atagacgtta cttgacaggc ttcgccgctt gccgctacaa cggcgcaaca ggagaaggag
      901 tgtgattatg aagcgattag cctttctcgg attcttgacg gtgacgttgt tttccagcgt
      961 cacagcgtgg gcgcagaagc cgctgccgac ggtgcgcatc ggcatcgaag cgccggccgg
     1021 gaccaacagt cattatcatg tcaccaagca ggccgggctg tttcaaaagt acggcctcaa
     1081 catggagctg atctcttttc ccggcggcac cgtgggcatg cagtcgctga tcgccggcga
     1141 tatccaattc gccactcaag acggcgtggc gacgctgacg gcaaatctgc gcggcgcgaa
     1201 tatttatttc gtcggcggca tgatcaacac cttcccattc cttatccttt cccgccccga
     1261 gatcaaggtg ccggcggact tgcgcggcaa gaaaatcgtc atcagccgct acggctcttc
     1321 gtccgatacc gcggcgcgcg ccgcggtgga gaagtatgga ctcaagcccg acaaggatgt
     1381 gattattttg cagggcggcg gtcagagcga gcgcttcgcg gcgttgaaag ctggcgtggt
     1441 cgacgccgcc atcgtcagcc cgccgctcaa tctcgccggc cggcaattgg gtttcaacga
     1501 agtcatcgat ctgtcggatt ccggggttcc ctacgcgcac caattgatcg tcgccaccaa
     1561 ggattttctc gatcgcaatc ccgacctgac catgcgcacc ttgcgcgcgc tcatcgaagg
     1621 actgggcctg tggaaagatc cggcgaagaa gccacaagtt ctaaaatcca ttggccacta
     1681 tttgcgcctc gacccggaga agcaaaaaga ccagctggaa gaaacctacc gctactacag
     1741 taaaaccttt ccgaccaaac cttatccgac cctcgaaggc gtcgagttca ccgcgcagat
     1801 cttaaaaagg aaccgcccgg aggccaaaga tatacaagcg aaggattatg ttttgtacaa
     1861 gtatgtcgct gaattggaga aggaaggttt tttaaccaag gtgtttggcg gaaagtagac
     1921 tcaaaagtca cgactaaata gtctggagta aagagtgcgg aatccgggac cgcattacta
     1981 cttactcctt agcgccgggc gcgcgcgtgg cgatcatcag actgcattcg ttgccataac
     2041 ctttgaccga gatcatggag ccttcgcctt cgactccggt gcgaacgcct tttcttatcg
     2101 ggacatcctg actcttaaca tgaagtggca tagtggtttc cgctcctgga tgggattttc
     2161 aaatgcttct acgatcgcat cgctgaccat caccgttcga tcagtatggg ccgccggcgc
     2221 gcttcctggc cgcgtggaag gcttccgccg ctttattaat cacctcttcg gtgtcgatgg
     2281 cgactaactc gcccttatgt ttgagaattc tgccgtcgat cacgacggtg tcgacgttga
     2341 aagggttggc ctgctggacg agtagaagcg ccgggttgat gaacggcgcg acgttgatgt
     2401 cgttggttct gactaaaatt aaatcggctc gcttgcccgg cgtcaacgag ccggtgcggt
     2461 cggcgatgcc gaggtctttt gcgccgtcga gggtagctag ttccaagatc cttcgcggct
     2521 ggacttccat cacgtccatc gagcgcaggt gattgagctg cagcatcagg cgcatgatgc
     2581 tgaacatatc ggcattgccg cccaccgtcg tcgtgtccac cgaca
//
LOCUS       DASQKS010000513         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5739754, whole genome shotgun sequence.
ACCESSION   DASQKS010000513 DASQKS010000000
VERSION     DASQKS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5739754"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VE170_13200"
     CDS             complement(<1..843)
                     /locus_tag="VE170_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16444.1"
                     /translation="MKNKFFPLAIIALLALLLNAPHVFAQVAHPELVAQAKKEGRVVW
                     YTTVSIPESKQLIDLFERQYPFIKVDLLRSGAGALVNRVVSEYAARTYAADVLQGTAS
                     RGGLSLLKKREILGRYESPEFKYLPADLRDKAGYWGSTFQNTFVLAYNKRNVRPDDVP
                     KSYDDLLKPMWKGRQIINDTDNFEWFDGLLKAWGKDKGLAYFRRLAAQDQIFQRGARG
                     RISLVAAGEAPLTIGYGPHAQSFVNQGAPIDWVPLEPVVVIINTVSIAARPPHPAAAK
                     LFIDF"
     gene            complement(869..1819)
                     /locus_tag="VE170_13205"
     CDS             complement(869..1819)
                     /locus_tag="VE170_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYJ16445.1"
                     /translation="MSLINYRDAVGKALQDSMREEPRLIVIGQNLAAHALKPLAETYG
                     LDRVRDCSISESAMVGMAVGAAMKGMKVVVMIAYADIATVCHMAIVQSAAKLHYLTNG
                     RLHCPVIIRLPIARFRGHGPEGNEVGVSWFYNVPDLNITMPGSPSQAYWSFREALTRP
                     TPTLFFEDRSIHLRAGELAANNPGGRAAITRAGERLTIIGAGRAAALAEDAADELSKR
                     GFGGAVEVVSLGFIKPCDTETVLNSAGKTGRVLIVQDEPEWAGYAPYVRCALDRLPPG
                     SLAMAPRMLVGADQYLPYWDERPFLPSLENVVSAATELLK"
     gene            complement(1869..>2190)
                     /locus_tag="VE170_13210"
     CDS             complement(1869..>2190)
                     /locus_tag="VE170_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ16446.1"
                     /translation="ASRQVDGNDAMDVYQSARWARATALGGQPVFLDCLTFRMGLYSS
                     HFGEVRPGIENDLAEAAKRDPLKRLGDWLISNGMASADDLARLQRVEERRIEAAFAHV
                     AAEL"
BASE COUNT          441 a          703 c          622 g          424 t
ORIGIN      
        1 aaagtctata aacagcttgg ccgccgcggg atgaggcggg cgcgcggcga tacttaccgt
       61 attgatgatc accaccaccg gctccaaagg tacccagtcg atgggcgcgc cttgattgac
      121 gaagctctgc gcatggggac cgtagccgat ggtcagcggc gcttcgcccg ccgccaccag
      181 cgagatgcgt ccgcgcgcgc cgcgctgaaa gatctggtcc tgcgccgcca gccgccggaa
      241 gtaggccaga cccttgtcct tgccccaggc cttgagcaga ccgtcgaacc attcgaaatt
      301 gtcggtgtcg ttgatgatct gccgtccctt ccacatcggt ttgagcagat cgtcatagct
      361 cttcggcacg tcatccggcc tgacgttgcg cttgttgtag gcaagtacga aggtattctg
      421 aaaggtcgag ccccaataac ccgctttgtc gcgtaaatcc gccggcagat atttgaactc
      481 cggcgattcg taacggccaa gaatttcccg cttcttgagc aagctgagac cgccgcggct
      541 cgccgttcct tgtaacacgt cggcggcata cgtgcgcgcc gcatactcgc tcaccacgcg
      601 gttgaccaac gcccccgccc ccgagcgcag cagatcgact ttgatgaacg gatactgccg
      661 ctcaaacaga tcgatgagct gcttgctctc cgggatcgaa accgtcgtgt accagaccac
      721 gcggccttct tttttcgcct gggcgaccag ctcgggatgc gcaacctggg caaagacatg
      781 cggcgcgttt agcaacaatg ccaatagtgc aatgatcgcg agtgggaaaa atttattctt
      841 catctttata atcccgtccg gcacaatctt actttaacaa ttccgtggcc gcgctcacga
      901 cattctccag cgacggcaaa aacggccgct cgtcccaata gggcaaatac tgatcggcgc
      961 cgaccagcat gcgcggcgcc atcgccaagc taccgggcgg caagcgatcg agagcgcagc
     1021 gcacataggg cgcatagccg gcccattcgg gttcgtcttg aacgatcaag acgcggccgg
     1081 tcttgccggc ggagttcaaa accgtctcag tgtcgcaggg tttgataaat cctaaactaa
     1141 ccacttcaac cgcgccgccg aatccccgct tcgatagttc atcggcggca tcttcggcaa
     1201 gcgcagccgc gcggccggca ccgatgatcg tcaagcgctc gccggcgcga gtgatcgccg
     1261 cgcggccgcc agggttgttc gcggccagct caccggcgcg taggtgaatc gagcggtctt
     1321 cgaaaaacaa ggtcggcgtc ggccgcgtca gcgcctcgcg gaaactccag taggcctggc
     1381 tcggcgagcc cggcatggtg atattgaggt ccggaacgtt ataaaaccac gacacgccga
     1441 cctcattacc ttccggacca tgaccgcgaa acctcgcgat cggcaaacga atgatgaccg
     1501 gacaatgcaa ccggccgttg gtgagataat gcagcttcgc cgcgctttgc acgatcgcca
     1561 tgtggcagac cgtggcgata tcggcatagg cgatcatgac gacgaccttc attcccttca
     1621 tcgccgcccc gaccgccatg ccgaccatgg cgctctcgct gatcgaacaa tcgcgcacgc
     1681 gatcgagtcc ataggtctcg gctaacggtt tcaaggcatg ggccgccaga ttctgcccga
     1741 tgacgatcaa gcgcggttcc tcgcgcatcg aatcttgcaa ggctttgccg actgcgtcgc
     1801 ggtaattaat tagggacatc ttgggcgttt ccttttgtat ttgtccgtga gagcggcacc
     1861 ggccagcgct acaactcggc agcgacgtgg gcgaacgctg cttcgatgcg acgctcttca
     1921 acccgctgga gccgcgccaa gtcgtcggcg cttgccatcc cgttgctgat cagccaatcg
     1981 cccaatcgct tcaacgggtc gcgcttggcc gcttcggcta aatcgttttc gatgcctggg
     2041 cgcacttcgc cgaaatgcga gctatacagg cccatgcgga aggtcaggca atcgagaaac
     2101 accggttggc cgccgagcgc cgtcgcccgc gcccagcgcg ccgactgata aacatccatg
     2161 gcatcgttgc catcgacttg gcgcgacgcc
//
LOCUS       DASQKS010000514         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5739937, whole genome shotgun sequence.
ACCESSION   DASQKS010000514 DASQKS010000000
VERSION     DASQKS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5739937"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VE170_13215"
     CDS             <1..1015
                     /locus_tag="VE170_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYJ16447.1"
                     /translation="CHPGDDSAGGVVQLLLDALPSAALLTDGSGKIIMLNQQAERFLG
                     WPAAKLIGQPAHEWFECYLEELAAEPENCPIARTLQGENIASPGRMRLHCRGSVAKPV
                     EYHCSRYPTGSGVGVILAFNDITRELAVEKDLRGLASIAEACPLAIVELNEDANLIHA
                     NPAMMRLMDRFGFGAGLHAAVLPENIAALTADCLARQSEIGAIEVTFGDHCYEWKLVP
                     VTGERVVRGYGVDLTARKRTELALERSTTEAESASRAKSELLANVSRQLRTPLNHVIG
                     MAALLVDSELTEDQRGCAETIQTCAASLMGVVEDLLTTADQPGEDRTPNHRSSAVPLA
                     CEK"
     gene            1215..1967
                     /locus_tag="VE170_13220"
     CDS             1215..1967
                     /locus_tag="VE170_13220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16448.1"
                     /translation="MADHDLTGQVALITGGATGIGRAMAQALGNAGAKVWLASRDAAA
                     IERSVAELRINGITVHGAQLDVTDRGQVNNLVGEIVDRHGKVDILINSAGVMIKYPLL
                     DMPENVWDQVIGINLKGTFLCSQAVARGMIAQGYGRIIHFTSGHVKGNATNSAYSASK
                     GGVEAFTRSVAAEFKQLGVDVTINAVEPGGTDTPMWRRGKSAEYIKQSLDSGRIRTAD
                     YMAATIVYLASRESAAVTGQVIERGTKPPLGS"
     gene            complement(2032..3114)
                     /locus_tag="VE170_13225"
     CDS             complement(2032..3114)
                     /locus_tag="VE170_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ16449.1"
                     /translation="METCNAAVITEHNKPLTIQKVQIPNLDPGSLLIKITASTLCGTD
                     VHRWHGPLGDKDSLPIVTGHEPCGTVQDIAGERTDILGNPVKRGDRVVWSYVACGSCY
                     YCTVACQPCICRGRASWGHNRSDQHPYLLGSCAEYMYVPKECLIIKVPDEVTSASAAA
                     SACAYRTVMHGYDRLGAIKSHETVVIQGSGPLGNFATAVAKDHGAKKVLVIGAPANRL
                     EVTKKMGADAVLNLEEVIDEKQRRQWVKDHTDGRGADVVIQVANNMAVPEGLTLLRDG
                     GRYLNIGAGGKANIAVEKIPQEMLFITIRSGEPRHWLQAIDFLASRRKTFPFEEMISK
                     SYKLDQINEAMTAMANYSVVKAVINF"
     gene            complement(3231..>3731)
                     /locus_tag="VE170_13230"
     CDS             complement(3231..>3731)
                     /locus_tag="VE170_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16450.1"
                     /translation="MDHMNMYVPVHALGHPWGLTINCADILYNGIYERFPRVRIAFLE
                     GGIAWLLLLLERLHASHETHFQHIPEGEFGIREQQDPAKALRKLIKDGRIYLGIETEE
                     LTMPFAIKTVGNSPFLYSSDFPHEVTHESCKHDLAELMESDEITADDKAAMLWRNSET
                     FYKLPL"
BASE COUNT          738 a         1015 c         1156 g          822 t
ORIGIN      
        1 ttgccatccc ggtgacgaca gcgccggcgg tgtggtgcaa ctgttgctcg atgctttgcc
       61 gtcggccgcg ctgctcaccg atggctccgg caaaattatc atgctcaacc agcaagccga
      121 gcggtttctt ggttggccgg cggccaagtt gatcgggcag ccggcccatg aatggttcga
      181 atgttatctg gaagagcttg ctgccgagcc ggaaaattgt ccgatcgccc gcacgcttca
      241 aggtgaaaat attgcgtctc ccgggcgcat gcggctgcat tgccgcggct cggtagccaa
      301 acccgtcgag tatcattgct cgcgttatcc taccggcagc ggcgtgggtg ttatcttggc
      361 tttcaatgac atcacgcggg aactggccgt ggaaaaggat ctgcgcggtc tggcttcgat
      421 cgcagaggcc tgtccactcg cgatcgtcga actcaatgaa gacgccaacc tgattcacgc
      481 caatccggcg atgatgcgac tgatggacag attcgggttt ggcgccggcc tacatgcggc
      541 ggtgctgccg gaaaatatcg cggcgctaac ggcggactgc ttagcgcggc aaagtgaaat
      601 cggcgccatc gaagtcacct ttggcgacca ctgctatgag tggaaactcg tgccggtgac
      661 cggtgaacgg gtcgtccgcg gttatggcgt cgacttgacc gcgcgcaagc gcacggaact
      721 ggcgctggag cgctccacga ctgaagccga gtccgccagt cgcgccaagt cggaactact
      781 ggccaatgtg agccgtcagc tgcggacgcc gttgaaccac gtcatcggca tggcggcatt
      841 gttggtagac agcgaattga ccgaagatca gcgcggctgc gccgaaacca ttcaaacctg
      901 tgcggcgtcc ttgatgggtg tcgttgagga tctccttacc acggctgatc agccgggaga
      961 ggatagaacg ccgaaccatc ggtcgtcggc cgtgccactt gcgtgcgaga agtgagcgag
     1021 tcttgtggtg agatcttcgg ccgttccagt cgattcgtcc tgccgaacat gagtgccttc
     1081 atagaaggcg cactgctcta gttgccaatt ttttccactc ttcctatgat cggctgcgct
     1141 cgggggatcg cgcggcaatt gtcagcctgc gcagatttga cgtgcaaccc agccgatttt
     1201 gtaggagtga aatagtggca gatcatgatt taaccggaca agtggcgctt atcaccggcg
     1261 gcgctaccgg tatcggccgg gcgatggccc aggcactggg caatgccggg gcaaaggtct
     1321 ggctcgcttc gcgcgatgct gcagcgatcg agcggtcagt ggctgagctg cgcattaacg
     1381 gcattaccgt gcacggcgcg caactcgatg tcactgatcg cggtcaagtt aacaatctag
     1441 tcggcgagat tgtcgaccgc cacggcaagg tcgacatcct gatcaatagc gcgggcgtga
     1501 tgatcaagta tccgctgtta gatatgccgg aaaatgtctg ggaccaggtg atcgggatca
     1561 atttgaaggg gacattcctg tgctcgcagg cggtggcgcg tggcatgatc gcgcaggggt
     1621 acggtcggat tattcacttt acctccggcc atgtgaaagg caatgccacc aattcggctt
     1681 actcggcgtc gaagggcggg gtggaggcgt tcacgcggag cgttgccgcc gagttcaagc
     1741 aactcggcgt cgacgtgacg atcaacgctg tcgagccggg tggcaccgat acgccgatgt
     1801 ggcgccgggg taagagcgcc gaatatatta aacaatcgct ggattccggc cggatccgca
     1861 cggcggatta catggctgcg acgatagttt atctggcgag ccgcgaaagc gccgcggtca
     1921 ccgggcaggt catcgagcgg ggaaccaagc ctccgctcgg ttcttagtgc agaaagcagt
     1981 agtcaatacc gggcggatct aactcacgtc gcaagtcttt cgcttcatgc cttagaaatt
     2041 aatcacagcc ttgacgacgg agtagttcgc catcgctgtc atggcttcgt tgatctggtc
     2101 gagcttgtaa cttttgctga tcatctcttc gaagggaaag gtcttccggc gtgaggcgag
     2161 aaaatcgatg gcctggagcc agtggcgcgg ttcgcccgaa cggatcgtga tgaacaacat
     2221 ttcctgggga attttttcca ctgcgatatt cgctttaccg ccggcgccga tgttcaaata
     2281 acggccgccg tcgcgcagga gcgtcagacc ttcaggcacc gccatgttgt tggcgacctg
     2341 gatgacgaca tcggcgccgc gcccgtcggt atggtccttg acccattggc ggcgctgctt
     2401 ttcgtcgata acctcttcga gattcaagac cgcatcggcg cccattttct tggtcacttc
     2461 cagacgattg gcaggtgcgc cgatcactaa aacttttttc gcgccatgat ctttggcgac
     2521 tgccgtggcg aagttgccga gcggtccgct gccctggatc accacggtct catgactttt
     2581 aatcgcgccg aggcggtcgt aaccatgcat gaccgtgcgg taggcgcagg ccgaggccgc
     2641 tgctgacgcc gaggttactt cgtcgggaac cttgatgatc aggcattcct tcggcacgta
     2701 catatattcc gcgcagctgc caagaagata gggatgttga tcgctccggt tatgccccca
     2761 ggatgcgcga ccgcggcaga tgcaaggctg gcatgcgacg gtgcaataat aacaggaacc
     2821 gcaagcgaca tagctccaga cgacgcgatc gccgcgcttg accgggttgc ctaagatatc
     2881 ggtgcgctcg ccggcgatgt cttgaacggt accgcagggc tcgtggccgg tgacgatcgg
     2941 caggctgtct ttgtcgccca gcgggccatg ccagcggtgt acatctgtgc cgcacagcgt
     3001 cgaggcggtg atcttgatca gcaaagatcc cgggtcgaga ttggggatct gcaccttttg
     3061 aatggttagc ggtttgttat gttcggtgat gactgcggca ttgcaagttt ccatggagtt
     3121 tcttccttcc ttcgagcgcg ggtaacgttt ggaacttctg gaaccctcga aacgattgga
     3181 accgtttcat cagttcgaaa agttccaggg gttccactcg gatcgtcgat ttacagcggc
     3241 agcttgtaaa acgtctccga gttgcgccac agcatggcgg ctttgtcgtc cgcggtgatc
     3301 tcgtcgcttt ccattagctc agcgaggtcg tgcttgcagc tttcgtgggt tacttcatgg
     3361 ggaaagtccg acgagtagag aaacggtgaa ttgccgacgg tcttaatggc gaagggcatg
     3421 gtcagctctt cggtctcgat gccgagatag atgcggccgt ctttgatcag ttttcgcaga
     3481 gccttagccg ggtcctgttg ctcgcgaatg ccaaactcgc cttcgggaat atgttggaag
     3541 tgggtttcgt gcgaagcatg gagccgttcg agcagcagca acagccaagc gatgccgcct
     3601 tcgaggaagg cgatgcgcac gcgcgggaag cgttcataaa ttccattgta aagaatgtcg
     3661 gcgcaattga tcgtcaggcc ccagggatgg ccgagagcgt gcaccggcac atacatgttc
     3721 atgtgatcca t
//
LOCUS       DASQKS010000515         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_707292, whole genome shotgun sequence.
ACCESSION   DASQKS010000515 DASQKS010000000
VERSION     DASQKS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707292"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..2160
                     /locus_tag="VE170_13235"
     CDS             196..2160
                     /locus_tag="VE170_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16451.1"
                     /translation="MAQSRRQRKKPTNDPPPPTRSRSQRGGVAVENFHGARWTGVIVI
                     LHATAVALTFETIYALLGNQPLIDQDWGLHFHHLRSLQAYWRDSAGLSGYNPYFMAGY
                     PSNTIQDLSVKFFEFAALGLSTIALAPVQWFKIVAFLATASVPVVMFYAGRNFFYHHM
                     FRDKIAVVSALLGTLYWWNSLPREMFFYGMIGFPAAAYCSVWGVSLFYRLVKDSPKPG
                     LLHLGWLLFAAVILPLHVQSLVIFMPFMIALLIVPARRITGTLCFWLVAALAVGALIN
                     FPWLTTAFQHRHDDVSSAIVDQLPLFASSDPLTFLIDYLGAKGYWTFRPSFVEKGFRL
                     MLLILGAVGTRELIKQDNRPVGVALTAGLSVLFLLTYFGAFIPGLAAWQPLRFKVPLD
                     LFLVIGAAFCLNQWLTRQQALRFYLVPATFALGGLALVINIVQTESTGKLQLRADMRP
                     EITAIVDWVGRETPGDGRVLFEESGDETGFVYDGMYLSSFLPHLSGRQLIGGPINIYN
                     DRHHFAEFHSGQLFKRDIGAISDDDLRNYLRLYNIGAVVAFHPASIKKLLSIPGLVTV
                     ERRIGPIHLMKVNQPLSWFVEGDGKVQAGANRLELRNLKGSEILLKYHWVVGLNSEPA
                     ATIEPVRLADDPIPFIKLIAPPASLVLRVQ"
     gene            complement(2191..4116)
                     /locus_tag="VE170_13240"
     CDS             complement(2191..4116)
                     /locus_tag="VE170_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ16452.1"
                     /translation="MRFAHLWQIETIPLFYNLAGDGRTYDEWAQRIAAGDWLGSGVFY
                     QAPLYPYFLAVWQFVFGHDLWLIRCLQILLGALSCATLYLVSAKLFSRSAGIATGLVL
                     AIYAPAIFFDGLIEKSILDLALLCLMLFLLVDVVRTHDLMKWPAVGSLLGLLGLSREN
                     ALILAPIVALWIFVGFPRELPAIRARWLGYFLTGLLLVLLPVGIRNLIVGGEFTLTTS
                     QLGPNFFIGNNPVADGTYGSVRQRIKEVQLEGPDAKRLAERASGHALTPAEVSTYWLR
                     QSIDFIETQPAQWLSLLARKWLLVWHGREIEDSDDFYIYRQFSTPLMLLAWFAHFGVL
                     APLAAAGVFFTRREWRRLWLFHAMILALAGSTALFYVFGRYRYPLVPLLALFAGAAIV
                     EFVRLLRQKNWSRGIQAAIVLAITAGVVNWPIGASGPGAAGYNNLANAFSKQGNVDAA
                     IIAALRALEIQPDYGVAHYNLGNLYMQQGRFDLARRHFEDALRLLPNYAEAHSNYGQL
                     LAEGGDLALGLEHFQKAIVLNPSVSRAQLNLGVALAKNGRMDEAVAPLQRAAALSPEA
                     AEPVYYLGSVYAAQHRYAEAEAAFTQALQRRGDFAPAHQSLAQLLALQGKKAQALQHH
                     QEAVRLMEQSGSSAPAR"
BASE COUNT          936 a         1255 c         1170 g          911 t
ORIGIN      
        1 aaaccgatcc tggagagaaa caggatcttg ccgttgccga gccgctgatc gtgaaaaatc
       61 tcactgcacg gctaaatacc tggcgtaagc tccaaatcga ttactattcg gatgccacgc
      121 tgcaaaaacg cgagtaccca cccatactca gcgattaact gctgatcgac ctcatcctaa
      181 agcgcatggt gcaacttggc acaaagccgg cgacaaagaa aaaaaccgac caacgaccca
      241 ccgccgccga cgcgctcacg atcacagcgc ggcggggtcg cagtagagaa ttttcatggc
      301 gccaggtgga cgggtgtaat cgttatcctc catgcaacgg ccgtcgccct gacttttgaa
      361 acaatttacg cgctattagg caaccagcca ctgatcgatc aggactgggg gctgcacttt
      421 caccacttgc gatcactgca ggcgtactgg cgcgacagcg ctggtttgtc gggctacaac
      481 ccatacttca tggccggcta tccatcgaac acgatccaag acttgagcgt caaatttttc
      541 gaattcgctg cactgggact ttcgaccatc gcattggcgc cggttcaatg gtttaagatc
      601 gttgcctttc tagccacggc cagcgtaccg gttgtgatgt tctacgctgg gcgtaatttt
      661 ttctaccatc acatgttcag ggataagatc gccgtcgttt cagccctgtt gggaaccctg
      721 tactggtgga attcgttgcc gcgagaaatg tttttctacg gcatgatcgg ctttcccgca
      781 gctgcttatt gcagcgtctg gggcgtgtcg ttgttctacc gtttggtcaa ggattcgcct
      841 aaaccagggc tactgcacct gggctggctc ttgttcgcag cggtgattct accactgcac
      901 gtccagtcgt tagttatttt catgccattt atgatcgcgc tgctaatcgt cccagcccgg
      961 cgcattaccg gtacactgtg tttttggttg gttgcggcgc tggcagttgg cgccctgata
     1021 aacttccctt ggctcacgac cgcgttccag caccgccacg atgatgtttc ttcggccatc
     1081 gtcgatcagc taccgctatt tgccagcagc gatccgttga cattcttgat cgactatctc
     1141 ggcgccaaag ggtattggac attccgtcct tcgtttgtcg aaaaaggttt tcgcctcatg
     1201 cttttgatct tgggcgcggt cgggacccgg gagttaatca agcaagacaa tcgccccgtc
     1261 ggcgtagccc tcaccgccgg tctatcggtg ttattcttgc tgacctactt cggcgccttc
     1321 attcctggcc tggcggcttg gcagccgttg cgtttcaagg taccgctcga ccttttcctg
     1381 gtgatcggcg cggcgttttg ccttaatcaa tggcttactc gtcagcaagc cttgcgcttt
     1441 tacctcgtgc cggcaacgtt cgccttgggc gggttggcct tggtaatcaa tattgtgcag
     1501 acagaatcga cgggcaagct gcaactgcgt gccgacatgc gccccgaaat cactgcgatt
     1561 gtcgactggg ttggccgtga aaccccaggc gatggccgag tgctgttcga agaatccggc
     1621 gatgaaaccg gctttgtcta cgacggcatg tatctgtcgt cttttctgcc ccatttgagc
     1681 ggccggcagt tgatcggcgg gcccatcaat atttacaacg accgccatca tttcgccgag
     1741 tttcattcgg gtcagctttt taagcgcgac atcggcgcca tttcggacga tgacctgcgt
     1801 aactatttgc ggctgtacaa catcggcgcg gtagtcgctt tccatccggc ttcgattaaa
     1861 aagctgctgt cgatacctgg tctcgtgact gtcgagcggc gcatcggacc gatccacctc
     1921 atgaaagtaa atcagcccct gagctggttt gtcgagggcg acggaaaagt ccaagccggc
     1981 gccaatcgct tggagttacg caacctgaaa ggcagtgaaa tcctgctcaa atatcattgg
     2041 gtggtcggcc ttaacagcga gcccgccgca acgatcgaac cggtcagact agccgacgat
     2101 ccgattccgt ttattaaact catcgctcca ccagcgtcat tggtcttgcg agtccaatag
     2161 ctcggcttgt cggcacataa catcgagcag ctatctagcc ggcgcactcg acccgctttg
     2221 ctccatcaag cgaactgctt cctgatggtg ttgcagcgcc tgcgcttttt ttccttgaag
     2281 cgctaacagc tgcgccaggc tctgatgcgc cggcgcaaaa tcgccgcgcc gttgcagcgc
     2341 ctgggtaaac gccgcctccg cctcggcgta ccgatgctgc gccgcgtaga cgctgcccag
     2401 ataataaacc ggctcggcgg cctccggact gagcgctgcc gcccgctgca gcggcgccac
     2461 cgcctcgtcc atccggccgt tttttgccag agcgacgcca agattgagct gcgcccggct
     2521 caccgaggga ttgagcacga tcgccttctg gaaatgttcc agtcccagag cgagatcgcc
     2581 gccctcggcg agaagttgcc catagttgct atgggcttcg gcatagttgg gcaataaccg
     2641 gagcgcatct tcaaaatggc gccgcgccag atcgaatcga ccctgctgca tgtacagatt
     2701 gccaaggtta tagtgggcca caccgtagtc cggttggatc tccaaggcac gtaaggcagc
     2761 gatgatcgcc gcatcgacat tgccctgctt cgaaaaggca ttggccaagt tattatagcc
     2821 ggcggctcct ggcccggatg cgccgatagg ccaattcacg accccggctg tgattgccag
     2881 aacgatcgcc gcctggattc ctctggacca atttttttgg cgcaacagcc ggacaaactc
     2941 gacaatcgcc gccccggcaa atagcgctaa taacggcacc aacggataac gatagcggcc
     3001 aaagacatag aaaagcgccg tactgccggc gagggccagg atcatggcat ggaacagcca
     3061 gagccgccgc cactcgcgcc gggtgaaaaa caccccggca gcggcaagtg gcgcgagcac
     3121 gccgaagtga gcgaaccacg ccaagagcat caatggcgtc gagaactggc ggtagatgta
     3181 aaaatcatcg gagtcctcga tctcgcgccc atgccaaacc aataaccact tccgcgccaa
     3241 gaggctcagc cattgggccg gttgggtttc gataaaatcg atggattggc gcaaccagta
     3301 agtggaaacc tcggctgggg tgagggcgtg gccgctcgct cgttcagcca gacgtttggc
     3361 gtccggacct tcaagctgca cttctttgat gcgttgccgg accgagccgt aggttccgtc
     3421 ggcaaccgga ttattgccga tgaagaaatt cggccccaac tgggaggtgg tgagcgtaaa
     3481 ctcgccgcca acgatcaagt tgcggatccc gacgggcagc aaaaccaata atagacccgt
     3541 taggaagtaa cccagccagc gtgcgcggat cgccggcagt tcgcgcggaa acccgacaaa
     3601 tatccatagg gcgacaattg gcgctaagat cagggcattc tcgcgcgaca gccccaaaag
     3661 tcctagcagt gacccaacgg ccggccattt catcaggtca tgggttcgta cgacatctac
     3721 caggagaaaa agcatgaggc agagcaatgc caaatcaaga atactttttt cgatcaaacc
     3781 gtcgaaaaat atcgccggtg catagatggc gagcaccaag ccggtggcga tacccgccga
     3841 ccgagaaaac aatttcgcgc taacaagata aagcgtggcg caggacaacg cgccgagcag
     3901 gatctgcaag caacgaatca gccaaaggtc gtggccaaaa acaaattgcc agacagcgag
     3961 aaaatacgga tacaacggcg cttggtaaaa taccccgctg ccgagccagt cacccgcggc
     4021 gatccgctgc gcccactcgt cgtatgtgcg gccgtcgccg gcaaggttgt agaagagcgg
     4081 tatcgtctcg atctgccaaa gatgagcaaa tcgcacaagc agcgcgacgg caaagattgc
     4141 actgaaccac cgagccgtta ttctgggcgc gaggcgatcc gctgccgcgc ttggttggcg
     4201 gcggcgttgg gcccgcgtct ggtcagccaa tcgatctgat cgctgaacca tggcccaatc
     4261 gttttatgcc gg
//
LOCUS       DASQKS010000516         6705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4066605, whole genome shotgun sequence.
ACCESSION   DASQKS010000516 DASQKS010000000
VERSION     DASQKS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4066605"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VE170_13245"
     CDS             complement(<1..368)
                     /locus_tag="VE170_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16453.1"
                     /translation="MKRSLLLSMGLFLSAIFVHSPDVHAQAKPLRELKVGYPLGGSAS
                     YFWVAHKSGSFEKHGIKVEPIYIRGGLMGIQAALSGDLSLQLQGASTVVAAWAQGAKE
                     LQFIGAVGNRLDYILAAHPS"
     gene            complement(397..795)
                     /locus_tag="VE170_13250"
     CDS             complement(397..795)
                     /locus_tag="VE170_13250"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HYJ16454.1"
                     /translation="MLPYPPDQKTRARRLRSDITDAEKYLWAGLRDRQLCGAKFRRQH
                     PIGPFITDFCNVERGLVVELDGGQHEEQGAADRARTDFIESRGYRVLRFWNNDVLTNR
                     AGVIERIGEALESPHPSLSHRARVKRTLRR"
     gene            complement(983..1960)
                     /locus_tag="VE170_13255"
     CDS             complement(983..1960)
                     /locus_tag="VE170_13255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16455.1"
                     /translation="MKARNIHAWLAIFVVTALLLSVRSQAAQKELQKLTVGYTPISGA
                     SIPLFIALEEKLFHKYGYDVSGIFMGGSPLINSAILAGEFPLGYTGGGAIISSRLAGS
                     DLIAIASPLQVLTIDGWARQEIKSIADLRGKRVGVTRFGASTYFAALSMLESAGVKPG
                     EVTFIQNGGVGESFAALTGGRVDVCMIGYPYGLRAKNAGFHLLFRPLTTDYGLFPTAA
                     IAARDSWLKDARNRKVAVDFLRALGEGVMLARENAAVTKKALRKFTRVNDEADLQGSY
                     DFYKDAFPRTLRVDEKAMANALKFVEHPKAKGADVKQFYDNSLVEEAAK"
     gene            complement(2091..2468)
                     /locus_tag="VE170_13260"
     CDS             complement(2091..2468)
                     /locus_tag="VE170_13260"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HYJ16456.1"
                     /translation="MPVPQELKNRARELRSNQTDVEAKLWRRLRDRQVFGAKFRRQHP
                     IGSYIVDFCCPTLRLIVELDGGQHAEQNAADQARTNFLESRGYRVLRFWNNQVLTQLD
                     DVLEEISRRCVDPHPSLSLKGRG"
     gene            complement(2538..2957)
                     /locus_tag="VE170_13265"
     CDS             complement(2538..2957)
                     /locus_tag="VE170_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HYJ16457.1"
                     /translation="MAEARAPQKSLPKINPVDRPFWQGAAKNKFLLQKCNSCAKVQFF
                     PRIACVDCFGEMSWIEAKGTGKIHSFTLVRVPRNPAFKDEAPIYYINVILDEGVIIES
                     RLLGDNKEKVKMGDRVKVRYEQTHDPEIKLPCFELVG"
     gene            complement(2957..4108)
                     /locus_tag="VE170_13270"
     CDS             complement(2957..4108)
                     /locus_tag="VE170_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYJ16458.1"
                     /translation="MRRKKVCFVGYGSTEYSRKSEESLLGQYAIAIRAALHQTGLKKK
                     DINGLSVTTQASPDTAPHVAEQLGFELDWVLNADYGGASGVGAVSRAADAIECGHLDI
                     ALLLAGNSFDRSVAHQRQLEYQRGNFVDVYGYGGPNSLMALIQRLHMENFGTTLEQVG
                     KIAVAQRFNAINNPQALFREPMSMADYLNSRMISDPVRLFDCVMPCSGAECIILASEE
                     KAKQLTDKLVYLVTDAEKIHYQVANMVPEKTTFGMKLVGEKIFTEVKREDIDLFEIYD
                     DYPIAVMIQIEDFGYCKKGDGGKFVDAHDLTYKGDFPVNTGGGELSVGQAGLAGGFLH
                     VVEALRQLRGEADGHQVAKAERALVTGIGWLNYGRNLGTTSALVMERRK"
     gene            complement(4231..5358)
                     /locus_tag="VE170_13275"
     CDS             complement(4231..5358)
                     /locus_tag="VE170_13275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16459.1"
                     /translation="MDLKLSEEEKLIKETAAQFVDRQLIAREGVYLKQSQPFLAPGAP
                     ERRVLDATTAADLTKKARDIGLWALELPEAMGGPAMSHVAQVLIHREFGRSVLPFAPV
                     AIPGFMFETKYGEALAAGELSLSLAFDEAHKSGSLSHLRTLYRQEPEGAILQDCRLDV
                     YAPQADLFLFPAREQGTQRLGLFVIARDAPGLNILDEADLTTDATVARMTLSHCRIAN
                     EQLLGYEYEAGATIANEQLRIAARSLGIGMRCLAASIEHAKNRVTFGRRLAERQAIQW
                     LLADLAIEIRTATWLTLEAAWCADNDLPYFDEAALAKKRAARMAFQAADSAIQIHGGY
                     GVCKEFPFEGFYREARLMRLLYGREEELNRAVGEDFVKGQG"
     gene            complement(5468..6676)
                     /locus_tag="VE170_13280"
     CDS             complement(5468..6676)
                     /locus_tag="VE170_13280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16460.1"
                     /translation="MDQAMNFRLSAEEESLRQAVAAFVHAELLPLETEFAYAPDIFEG
                     SRWKSRVKSSSDPEIKRYLEIMEGLQQRAEAQGLWHLDVPKAYGGLAVSNVAMIAVTE
                     ELEKCTIPFEFGNHVSNILFNCQGEQVDKFLWPCIRGEKTSAFGLSEPASGADPSMMQ
                     TTATPDGDYFEINGTKMFPTFADVADFVQLFARLPGTKGREGVTCFIIDTGTPGYRVA
                     RSIATIAGTEPCELVFEDCRVHKSQVLGEVGNGWALNQAWLGARRFQVGIRAHGMAQR
                     VLNAVAQVLRQDVKDTEKYSGTIGYFLGELAALKALTYSASWRADQKMDVRVDAAMVK
                     LFGTELAHKVIDFALEAMGPSALRKEHAIARAFRHARAQRIVEGPSEVQRHIIQRELF
                     RQGGGLLEID"
BASE COUNT         1387 a         2044 c         1829 g         1445 t
ORIGIN      
        1 atcgaaggat gcgcggccag aatgtagtcg aggcggttgc cgaccgcgcc gataaactgt
       61 aattccttgg cgccctgcgc ccaggcggcg accaccgtcg acgcgccttg caattgcagc
      121 gacaagtcgc cggaaagcgc cgcttggatt cccatcagcc cgccgcgaat ataaatgggc
      181 tcgactttga ttccatgctt ctcgaacgac ccggacttat gcgccaccca gaaataactc
      241 gccgagccgc cgaggggata accgaccttg agttcgcgca agggtttggc ttgggcgtgt
      301 acatccggtg aatgaacgaa gattgcggag agaaatagac ccatcgacaa taataaactt
      361 cttttcattg ccgctccttt gctgataaaa tcaaactcac cgtctaagag ttctctttac
      421 cctcgccctg tgggagaggg aagggtgagg gctctcaagt gcctcgccga tcctctcgat
      481 cacgccggcg cgattggtca aaacgtcatt gttccaaaag cgtaagacgc gatagccacg
      541 cgattcgata aaatcagttc ttgctcgatc cgccgcccct tgctcttcat gctgaccgcc
      601 atcgagttcc acgaccaaac cgcgctccac attgcaaaag tcggttatga acggaccaat
      661 gggatgttgc cggcggaatt tagcgccaca caattggcgg tcacgcaaac ccgcccaaag
      721 atatttctcc gcgtcggtaa tatcgctccg cagcctacga gcgcgagtct tttggtcagg
      781 tggatatggc agcatgcgga aatgccctca ccctgaccct cacctctgtc ctctctccca
      841 gagggagagg aagtaagaat ctgcaaaatc ggttcccttt ttcatctcca tctctttcct
      901 agcgtcaccc tcgccccctg ggagagggaa ggtgagggct catacgatcg ggcttcccgc
      961 cagctcaaaa cacgccagaa aactacttgg ccgcctcttc aaccaaactg ttgtcgtaaa
     1021 actgtttcac gtcagcgcct ttggctttgg gatgttcgac aaattttagc gcgttggcca
     1081 ttgccttttc atcgactctc aaggtccggg gaaaagcgtc cttgtagaaa tcgtagctgc
     1141 cctgcaagtc ggcctcgtcg ttgactctgg tgaattttct cagcgctttc ttggtcacgg
     1201 cggcgttttc tctggccaac atcacccctt cgccaagggc gcgtaaaaaa tccaccgcta
     1261 ccttacggtt cctggcgtcc ttcaaccaag agtcgcgcgc cgcgatggcc gcggtgggaa
     1321 atagtccgta gtcggtcgtc aacggccgga agagcaagtg aaacccggcg ttcttggcgc
     1381 gcaagccata gggatagccg atcatgcaaa catcgactcg gccgccggta agcgccgcga
     1441 aggattcgcc gaccccgcca ttttggataa acgtaacctc gcccggtttg actccagccg
     1501 attccaacat cgaaagagca gcgaaataag tcgacgcgcc gaaacgggtc acgccgactc
     1561 ttttgccgcg gagatcggca atcgatttga tttcctgccg agcccaaccg tcgatggtca
     1621 agacctgcag gggcgacgcg atagcgatca aatcgctgcc ggcgagccgg ctcgaaataa
     1681 ttgcgccgcc gccggtgtag ccgagcggaa attcgcccgc gagaatcgcc gaattgatca
     1741 gcggcgaacc gcccatgaag atcccggaaa cgtcgtagcc gtatttgtga aagagttttt
     1801 cctccaaggc gataaacaag ggaatcgacg cgccggaaat gggcgtgtag ccgaccgtga
     1861 gcttttgtaa ctccttctgc gccgcctgtg agcgaacgct cagcaacaat gccgtgacca
     1921 caaagatagc aagccacgca tggatatttc tcgctttcat ggcgttcctc cagtcttacg
     1981 attttggatt ttagattgga ggtcgcgtgt agggggccga cctgcgtgtc gtctcccagt
     2041 aggagaggga ataagtcaaa aacccggaag cgtgtttcat ttcccaatct tcaccctcgc
     2101 ccttttaggg agagggaagg gtgagggtcg acacaccgcc tgctaatctc ttctaacacg
     2161 tcgtccaatt gcgtcagcac ctgattattc caaaagcgca acacgcgata cccgcgcgat
     2221 tccaggaaat ttgttcgggc ttgatcagcc gcgttttgtt ccgcgtgctg tcccccatca
     2281 agttcgacaa ttaagcgcag agtggggcaa cagaaatcga caatgtacga accgatcgga
     2341 tgctgacgcc ggaacttagc gccaaacact tgccggtctc gtaaacgtcg ccaaagcttg
     2401 gcttcgacat cggtttggtt gctccgcaat tcgcgggctc gatttttcaa ttcttgagga
     2461 acgggcacac cctcaccctt gccctctccc gcaagcgggc gagggagtaa gaacaaattc
     2521 gatcgcttcc ctatacatca gcccaccagt tcaaagcacg gcagcttgat ttccgggtcg
     2581 tgggtctgct cgtagcgcac cttgacccgg tcgcccatct tgaccttttc tttgttgtcg
     2641 ccgagcaggc ggctttcgat gatcacgcct tcgtcgagga tcacgttgat gtaataaatc
     2701 ggcgcctcgt ctttgaacgc cgggttgcgc ggcacccgga ccaaggtgaa gctgtgaatc
     2761 ttaccggtgc ctttggcttc gatccaactc atttcgccga agcaatcgac gcaggcgatg
     2821 cgcggaaaaa attgcacctt ggcgcagctg ttgcactttt gcaggagaaa tttgtttttt
     2881 gccgcccctt gccaaaacgg ccggtcgacg ggattgatct tcggtaaact tttttgcggt
     2941 gctctcgctt cagccattac tttctccttt ccatgaccag cgcgctggtc gtgccgaggt
     3001 tgcgcccgta gttgagccag ccgatgccgg tgaccagggc gcgctcggct ttcgctactt
     3061 ggtggccgtc ggcctcgccg cgcaattggc gcagcgcctc gacgacgtgg agaaagccgc
     3121 cggccaatcc cgcctggccg acggataatt cgcccccgcc ggtattgacc ggaaagtcgc
     3181 ctttgtaagt aagatcgtgg gcgtcgacga acttgccgcc gtcgcctttt ttgcagtaac
     3241 cgaagtcttc gatttgaatc atcaccgcga tcggatagtc gtcgtagatc tcgaacagat
     3301 cgatatcctc gcgcttcact tcggtgaaaa tcttctcgcc caccagtttc atgccgaaag
     3361 tagttttctc cggcaccatg ttggcgactt gataatgaat cttctcggcg tcggtgacta
     3421 gataaaccag tttgtcggta agctgtttcg ccttctcctc ggatgccagg atgatgcatt
     3481 cggcgccgga gcaaggcatg acacagtcga acaagcgcac cggatcggag atcatccgcg
     3541 agttcaaata atccgccatc gacatcggct cgcgaaacag agcctgcgga ttgttgatcg
     3601 cgttgaagcg ctgcgccacg gcgatcttgc cgacctgctc caaggtcgtg ccgaagttct
     3661 ccatgtgcaa gcgctggatc aacgccatca gtgaattggg gccgccgtag ccgtagacat
     3721 cgacgaaatt gccgcgctga tactccaact gccgttgatg ggcgacgctg cggtcgaagg
     3781 agttgcccgc cagcagcaag gcgatatcga gatggccgca ttcgatggca tccgccgccc
     3841 ggctcaccgc gccgacaccg ctggcgccgc cgtaatcggc gttgagtacc cagtcgagtt
     3901 cgaagcccaa ctgctcggcg acgtggggcg cggtgtcggg actcgcctgc gtcgtcaccg
     3961 acaagccgtt aatgtctttc ttcttgaggc cggtctgatg caacgccgcg cgaatcgcga
     4021 tggcatactg gcccagcagc gactcctctg atttgcgcga gtattccgtc gacccatagc
     4081 cgacaaaaca aacttttttt cgtctcatgc ttttgctccc tctcgtctct ctggttcaag
     4141 cccattcaaa acgttcaaac ggttcaaacc ggttcagcgg gaagcggccc cgcggacgga
     4201 aatccaaaat tctctttagc gccttacgcc ttacccttga cccttgacga aatcctcccc
     4261 taccgcccga tttaactctt cctcgcggcc atagagtagc cgcatcaagc gcgcttcgcg
     4321 ataaaaccct tcgaagggaa actccttaca cacgccgtaa ccgccgtgaa tttgaatcgc
     4381 gctgtcggcg gcttggaagg ccatgcgagc ggcgcgtttc ttggccagcg ccgcctcatc
     4441 gaagtacggc agatcgttat cggcacacca ggcggcttcc aaggtcagcc aagtcgccgt
     4501 gcgaatctcg atcgccagat cggccaacag ccattgaatc gcctggcgct ccgccaagcg
     4561 ccggccgaag gtcacgcgat tcttagcatg ctcgatggac gccgccagac agcgcatgcc
     4621 gatccccaac gagcgcgccg cgatgcgcag ttgctcattg gcgatggttg cccccgcttc
     4681 atactcgtag ccgagcagtt gctcgttggc gatgcggcaa tggctcaacg tcatccgcgc
     4741 cacggtggcg tcggttgtca ggtcggcttc gtcgaggatg ttgagccccg gcgcgtcgcg
     4801 ggcgatgacg aacaaaccca aacgctgcgt gccctgctcg cgcgccggga aaagaaacag
     4861 atcggcttgg ggggcgtaca cgtccaagcg gcaatcttgc aaaatcgccc cttcaggctc
     4921 ctggcggtag agcgtgcgca ggtgggacaa gctgccgctc ttatgcgctt cgtcgaaggc
     4981 cagcgacagc gacagctcac cggcggccaa tgcctcgcca tatttggttt cgaacataaa
     5041 accgggaatc gccaccggcg cgaagggcaa caccgaccgg ccaaactcgc ggtgaatcaa
     5101 cacctgggcg acatgactca tggctggccc gcccatggct tccggcagct ccaaagccca
     5161 cagtccgatg tcgcgcgctt tcttggtgag gtcggcggcg gtcgtggcat cgagcacacg
     5221 ccgctccggc gcccccggcg ccaggaacgg ttgggattgc tttaaataaa cgccttcgcg
     5281 agcgatcagt tgcctgtcga cgaactgtgc cgcggtctct ttgatgagct tttcttcttc
     5341 gcttaacttt aagtccatcg gtcgcacgct tcccggcatt ttcgttgaac cttgaactct
     5401 tgaatccttc aacgaaacag tcggttcaag gttcaaaagt tcaagggttc aatcggatta
     5461 acagccgcta atcgatctct agtaaaccac cgccctggcg aaacagttca cgttgaatga
     5521 tatggcgctg cacttccgac ggtccctcaa cgatccgctg cgcgcgggcg tggcggaagg
     5581 cgcgcgcgat cgcatgttct ttgcgcagcg ccgagggtcc catggcttcg agtgcaaaat
     5641 cgataacttt atgcgccagc tcggtaccaa acagcttgac catcgcggcg tcgacgcgca
     5701 cgtccatttt ctggtcggcg cgccaggagg ccgagtaggt gagcgccttg agtgccgcca
     5761 actcgcctaa gaaataaccg atggtgccgc tgtatttctc cgtgtctttg acgtcctggc
     5821 gcaacacttg agcaacggcg ttgagcacac gctgggccat gccgtgagcg cggatgccga
     5881 cttgaaaacg gcgcgctcca agccaggcct gattgagtgc ccaaccgttg cccacttcgc
     5941 ccagcacttg cgacttgtgc acccggcagt cttcgaagac caactcgcaa ggctcggtgc
     6001 cggcgatggt tgcaatgctg cgcgcgaccc gatagcccgg cgtgccggtg tcgatgatga
     6061 aacaggtcac gccctcgcgc cctttggtgc ccggcaggcg ggcgaacaac tggacgaagt
     6121 cggcgacgtc ggcgaaggtc ggaaacatct tggtgccatt gatctcgaaa tagtcgccgt
     6181 ccggcgtcgc cgtggtttgc atcatcgatg gatccgcgcc cgacgccggc tcgctcaggc
     6241 cgaaagccga ggttttctcg ccgcgaatgc acggccagag gaatttatcg acctgttcgc
     6301 cctggcaatt gaacaagata ttcgagacat gattgccgaa ttcgaacggg atcgtgcact
     6361 tttccaactc ctcggtgacc gcgatcatgg cgacgttgct gaccgccaga ccgccgtagg
     6421 ctttgggcac gtccagatgc cacaagcctt gggcctcggc gcgctgctgc aacccttcca
     6481 tgatttccag gtaacgtttg atctccggat cgctgctcga ttttacccgg ctcttccacc
     6541 ggctgccctc gaaaatatcc ggcgcatagg cgaactcagt ctccagcggc agcagctcgg
     6601 catgcacgaa tgcggcgacc gcctgacgta aactctcttc ttcagccgac aaacgaaaat
     6661 tcatggcttg atccaatctc cctccgcccc tttatcacag cttgc
//
LOCUS       DASQKS010000517         7965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2816031, whole genome shotgun sequence.
ACCESSION   DASQKS010000517 DASQKS010000000
VERSION     DASQKS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816031"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VE170_13285"
     CDS             complement(<1..918)
                     /locus_tag="VE170_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16461.1"
                     /translation="MKSNRLLGIVTVLSYGLLALSAAYAEERVRVALSVRNVVFLPFY
                     YAKALRIYDKYGLNVELIQMRSDLQLAGLVSGEIDYTPSVGPAGTAISNSLPIKALAI
                     LYRAPLFSLVSPASIANMKELEGKKVAVSRIGSESHRYGSLIIESAGADPKKVTFIQT
                     GSTSISLAAIQQGTVNAAVLSPPFTGMMAEKGYKILGRSRSLAEAPWLGLVANRQKID
                     KQPEQVRNMLRSMRDVVAAMRRDRAGIINYIEKDFKVSSANATESYEDIMGVTVDNLT
                     LRDEQIQKYLDNSLARGEIAKRMTVNEMFD"
     gene            complement(924..1850)
                     /locus_tag="VE170_13290"
     CDS             complement(924..1850)
                     /locus_tag="VE170_13290"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ16462.1"
                     /translation="MVEQIRGIVLPSPTVFREDGKVDEPLMRELTDWFVACGVHAFFI
                     LGSYGQGAAMHPEERKNVAEMVVQQVKHRVPVVVHIGAVDPYTAIDLGKHAKAIGAEA
                     VGHVGPYYYADRSEFEIIEHLKMVDRAVGMPMLIYNNPRYSGYNIQPNFMARLVEAVP
                     NIFGAKLAMGSVDEAMAYMKIVKAPFAPYALASNLVAAMSVGVAGTISPPLAVTPEIG
                     VELVRAIDAGDATRAFLLQKNVIRIHDIFLRLAGPFGRTIYREAMRLRGFDVKMYPRW
                     PSKPLSREAYDEMRDLFTELNLIGAAGQLRSA"
     gene            complement(1933..2700)
                     /locus_tag="VE170_13295"
     CDS             complement(1933..2700)
                     /locus_tag="VE170_13295"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ16463.1"
                     /translation="MNIAIERGKKYSAQEAKAIVDGLYAKVRERWETRVNQKPFMQQL
                     TAGKLPHKTLKLFFRNWGAYTIEINTLTACTYHRNITFLKVNRDLMGPIGEKIADEFI
                     HPKPPGHFLVMMETAKALGLSEDEVFTSSMLSEFRGKIDFMRTIVYEGSAAEWYSAVT
                     TEEQIGHWSKACYQALTTHYGFDRAKATYFATHVEADLEEHEEGIMGHASFNRIALQR
                     LLETGMAEERPTYGLEYCAMTSVDLHGAMLRSAMEEA"
     gene            complement(2811..3758)
                     /locus_tag="VE170_13300"
     CDS             complement(2811..3758)
                     /locus_tag="VE170_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16464.1"
                     /translation="MAYRRTSLRWIIVLVALFAAVSMGDAAQPTKVRMSYSSRSNSNT
                     PFVIAYRKGFFAEEGMDVEMIQVNPRLGATALLNGDIDFTATFGTTLRGIVGGFPIKF
                     VAVSVKKSEHFLIVRPEIKEIRDLNGKKLGVATLFGSDQKAAEEMIRSKGFPPNIIKP
                     IALGEAPVRAQALRSGLVDAISVSSPFDLSLQAEGFRALAGPKDIDIALPTSGIAVAT
                     RLLQQNPQHVKRVVRALMKAHRFVFDNRKEVVPQMIRYLEQSPEVAERSYDLIVNSLS
                     RNGEITDAEWEVLTEKKKTADEVRDFSLLREVQKELKIR"
     gene            complement(3758..4894)
                     /locus_tag="VE170_13305"
     CDS             complement(3758..4894)
                     /locus_tag="VE170_13305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ16465.1"
                     /translation="MKNFAYIIVGAGFAGAATAYQLARQGVSDLLVLEQESIPGFHAS
                     GRNAAMLRQCIAEPDLTKLALDGAAFIRNLPADWPEPVLFKNNGSLLLGSGAGWEKLK
                     RDGATGRSVGVPGIAEWSPQQAIGRVPLLNQAEFDGALWCASDGVVDIHALLSGYLKF
                     AVSRGAQVRYNAAVRGVRARHGADIQLDTDEGVISGKVLVNASGAWANLVAELAGAQP
                     LPLRACRRHLFVSPPMAWVDRNWPFVWDVTHDIYFRPEGDGLLLCACDQTELAPGDPP
                     VDESVKEMLAEKIQRYMPGLSEVSISRGWAGLRTLTPDGRFVIGWDPKVDGLFWIAGL
                     GGHGMTTSAAVGELAAGLLVGGPGQRSTPFAPERFDDGSKGVQP"
     gene            5037..6863
                     /locus_tag="VE170_13310"
     CDS             5037..6863
                     /locus_tag="VE170_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ16466.1"
                     /translation="MRIHRYTQWDGTQEILFPTTDDIINQLSDHLLEEEGVRRALRDL
                     MRRGFTSEDGQRSVKGMRDFLRQAEEKRRELLHKYSPDSFKLTPEEQQKLTEKLGSLA
                     EKMEAYNEQMRNFMERMSGKFADRMDELSEKMQQAYQRYQELQQRLKQQIQERGMQGK
                     SNMSGEQNPQSLMDMLDRMSELLNNEDFLKNLPSMMDNAINNLESMLENMGNMTEEQL
                     QQMSDMMNQMQQLEQLMNQFPFQGSQRMGMGEAGQVLSQLRGLERFLRWGQRGMGDPS
                     DLNLDELRDILGEEAYENLKYLQNVEQMLEEEGYIVRGGHGLKLTAKGMRKIGDKALR
                     EIFQMLNKSRWGNHDTSARGSQGEHMEETKLYEYGDPMNINIGETVLNALGKRKPGEP
                     LRIHPEDFSVQRVQYSTVAETALLIDVSYSMLMNDALHAGKKVALALHRLIQTKYPQD
                     TLHLVAFRSNAKLIQAEDLPSIVSLTYFMEHGTDIKEALRYARQLLGSNRSANRQIIL
                     ITDGEPTAATLDRGGRLHSGWGSAMLHGRIVHETLKEVKRCTQSGIKINTFMLGADYY
                     RQGFVDQLSRLNIGRVFYTTPDQMGNYIMVDYLNNKKKRVGR"
     gene            complement(6956..>7965)
                     /locus_tag="VE170_13315"
     CDS             complement(6956..>7965)
                     /locus_tag="VE170_13315"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HYJ16467.1"
                     /translation="RTIGTDCVAGSSGAGVLTGAGEIEGDHGVAVMVFASDRFFARPF
                     IFEPLRGNEANLGETFGGFLAKAQDEKSLMLLFPDTYNGNAQNLLQAIAARAGFHPIA
                     GAGSSENGAAGATYQLCGDKLTTNALAGMYISGAFETHIDITQGCQPITPPMTITKAK
                     NNVILEIDNQPALPVFAKLLKGPLAEDLRRALTVLFVGLPADRSENSVAAGNYLVRNI
                     VGLDPDEGSVAVADQVSEGQSMIFTLRDGQRARDDLQEMLQRQLARLEGKTPAFGFYF
                     NCCARGSSLYGIPGIDAAYIKQALGDFPLIGMFGGFELAPLGRANHLFAYTGVLVLVT
                     E"
BASE COUNT         1748 a         2410 c         2194 g         1613 t
ORIGIN      
        1 atcgaacatt tcattgaccg tcatccgctt ggcgatttcg ccgcgggcca gtgagttgtc
       61 gagatacttt tgaatctgct cgtcgcgcag agtcaggtta tccaccgtca cgcccatgat
      121 gtcctcgtag gattcggtgg cgttggcgct gctcactttg aaatcctttt cgatataatt
      181 aattatgccg gcgcggtcgc ggcgcatcgc cgccaccaca tcgcgcatgg aacgcagcat
      241 attgcgcacc tgttccggct gcttgtcgat cttctgccgg ttagcgacca gaccgagcca
      301 gggagcttcg gctaaggatc ggctgcggcc gagaatctta taaccttttt ccgccatcat
      361 gccggtaaac ggcgggctca aaaccgccgc attaacggtt ccctgctgaa tcgccgctag
      421 actgatcgaa gtactgccgg tttgaatgaa cgtcaccttt ttcggatcgg cgccggcgct
      481 ttcgattatc agcgaaccgt agcgatggct ttccgagccg attcgcgaca ccgcgacttt
      541 cttaccttcc aactccttca tgttggcgat gctcgccggg ctgacgagcg aaaacagtgg
      601 cgcgcggtat aaaatcgcca gcgccttgat cggcagcgag ttggagatcg ccgtgcccgc
      661 cggtcccacc gaaggcgtgt aatcgatctc tccagacacc agccccgcca gctgcaaatc
      721 gctgcgcatc tgaatcagct cgacgttgag gccgtactta tcgtaaatgc gcagcgcttt
      781 ggcgtagtaa aacggcagaa agaccacgtt gcgcaccgat aacgcgacgc gcacccgctc
      841 ttcagcatag gcggcggata gcgcaagtaa tccgtagctt aaaacagtaa caatccctag
      901 cagacgattt gacttcatgt cgtctatgca ctcctcagct gacccgccgc gccgattaga
      961 ttcagttcgg tgaacaagtc gcgcatttca tcgtaggcct cacggctgag cggtttggac
     1021 ggccagcgcg gatacatttt aacgtcgaag ccgcgcagcc gcatcgcctc gcgatagatg
     1081 gtccggccaa atggaccggc caatcgcaga aaaatatcgt gaatgcggat cacattcttt
     1141 tgcagcaaaa acgcgcgcgt ggcgtcgccg gcatcgatgg cgcggaccag ctcgacaccg
     1201 atctccggcg tcaccgccag gggcggactg atggttccgg cgacacctac ggacatcgct
     1261 gccaccaaat tgcttgctaa tgcatagggc gcgaagggcg ccttgacgat tttcatatac
     1321 gccatcgctt catcgacgct gcccatcgcc agcttggcgc cgaaaatatt cggcaccgct
     1381 tcaactagac gcgccataaa gttgggctgt atgttgtagc cggaatagcg cgggttgttg
     1441 tagatcaaca tgggcatgcc gacagcacga tcgaccattt tcaagtgctc gatgatctcg
     1501 aactccgagc ggtcagcgta gtaataaggc ccgacatggc cgaccgcttc agcgccgatc
     1561 gctttggcgt gcttgcctag atcgatggcg gtgtaaggat cgacggcgcc aatatgcacc
     1621 accaccggca cccggtgctt cacttgctgg acaaccatct cggcgacgtt tttacgctct
     1681 tccggatgca tcgccgctcc ctggccgtag gagccaagaa taaaaaacgc atgcacgccg
     1741 caggcgacaa accaatcggt cagctcgcgc ataagcggct cgtcgacctt gccgtcctcg
     1801 cgaaacaccg tcggacttgg caacacgatc cccctaattt gttccaccat agaaacctcc
     1861 gaaacttatt cttcttttgc gttctttgcg gccaacattc cgattccgat tggggcgaaa
     1921 aatttttcgc ccctacgcct cttccatggc cgagcgcagc atcgcgccgt gcaggtccac
     1981 cgaagtcatg gcgcaatatt ccaatccata ggtcggccgt tcctccgcca tgccggtttc
     2041 gagcagccgt tgcaacgcta tacggttgaa gctcgcgtgg cccatgatgc cctcttcgtg
     2101 ctcttccaag tcggcttcga cgtgggtggc gaaataagtg gctttggcgc gatcaaaacc
     2161 gtagtgagtg gttagcgctt ggtaacaggc cttcgaccag tggccgatct gctcctcggt
     2221 ggtcaccgcc gagtaccact ccgccgccga accctcgtaa acgatagtgc gcatgaaatc
     2281 aatcttgccg cgaaattccg acagcataga cgatgtaaat acctcgtcct cgctaagacc
     2341 cagcgccttg gcggtttcca tcatgacgag aaaatggccc ggcggcttgg gatgaatgaa
     2401 ctcgtcggcg atcttctcgc ctatcggacc catcagatcg cggttcactt tgaggaacgt
     2461 gatattgcga tggtaggtgc aagcggtcag cgtattgatc tcgatggtat acgcgcccca
     2521 attgcgaaaa aacagcttca aggttttatg cggcagcttg cccgccgtca gctgctgcat
     2581 gaacggtttt tggttgacgc gcgtctccca gcgctcgcgc accttggcgt aaagtccatc
     2641 gacaatcgcc ttggcttcct gtgcggaata tttttttcct cgttcgatag caatgttcat
     2701 gtttcacctc gcgactagac gctacctctg agaaaaaacc ccgaacattt gcgtcaggtg
     2761 gtgtcctatc caggcggacg ctggaagacg gcccaacgct tggcgttccg tcaccgaatc
     2821 ttcaattctt tctgcacttc gcgcagcagc gagaagtcgc gcacctcatc cgccgtcttc
     2881 tttttctcgg tcagcacttc ccactcggca tcggtgatct cgccgttgcg gctgagcgag
     2941 ttgacgatga gatcgtagga ccgttcggcc acttccgggc tctgctccag ataacggatc
     3001 atctgcggca ccacttcttt gcgattgtcg aaaacgaatc gatgcgcctt catcaaagcc
     3061 cgcaccaccc gtttgacatg ctggggattc tgttgcagca agcgcgtggc gactgcgatg
     3121 ccggaggttg gcaaggcgat gtcgatgtcc ttaggacccg ccagggcgcg aaaaccctcg
     3181 gcctgcaggc tcaagtcgaa gggcgaggaa accgagatcg catcgaccaa cccggagcgc
     3241 aacgcctgcg ctcgcaccgg cgcttccccg agcgcgatcg gtttgattat gttcggggga
     3301 aaacctttgc tgcggatcat ttcttcggcg gctttttgat ccgaaccgaa cagcgtcgcc
     3361 acgccgagtt tcttgccgtt gagatcgcga atctctttga tctccggtct gacgatcaaa
     3421 aaatgttcgg actttttcac agataccgcg acaaacttga tcggaaagcc gccgacaatg
     3481 ccgcgcaacg tggtgccaaa ggttgccgta aaatcgatat cgccattcag caacgcggtg
     3541 gcgccaagcc gcggattgac ttggatcatt tccacatcca tcccctcctc ggcaaagaag
     3601 cctttgcgat aggcgatgac aaacggcgtg ttcgagttac tccggctcga gtacgacatg
     3661 cgcactttgg tcggttgcgc cgcgtcgccc atcgaaacgg cggcaaacag cgccaccagc
     3721 acaatgatcc accgaagcga tgttctacga tacgccatta cggctgcact cctttactcc
     3781 catcatcgaa tctttccggc gcaaacggcg tcgagcgctg gcccggcccg cccaccagca
     3841 accccgcagc caactcgccc accgcggcgc tggtcgtcat gccatgaccg ccgagaccgg
     3901 cgatccagaa cagcccatcg actttcgggt cccagccaat gacgaaacgg ccgtccgggg
     3961 tcagggtgcg caatccagcc cagccgcggc tgatggacac ttcggataaa cccggcatat
     4021 agcgttggat tttctcggcg agcatttctt taacgctttc gtccaccggc ggatcacccg
     4081 gcgccagttc ggtctggtcg caagcacaga gcagcaagcc gtcgccctcc ggacgaaaat
     4141 agatatcgtg ggtgacgtcc cagacaaacg gccaattgcg atcgacccac gccatcggcg
     4201 gcgagacaaa caaatggcgc cggcaggcgc gcaacggcaa tggctgtgcg cccgccaact
     4261 cagcgacgag gtttgcccaa gcgccgctgg cgttgaccaa aactttgcca ctgataacgc
     4321 cctcgtcggt gtccaactgg atatccgcgc cgtgccgcgc gcgcacccca cgcactgccg
     4381 cgttgtagcg cacctgcgcc ccccggctca cggcaaactt tagatatcct gacaacagcg
     4441 catgaatgtc gacgacgcca tcgctggcgc accacaaggc gccgtcgaac tccgcttggt
     4501 tgagcagcgg cacgcggccg atcgcttgtt gcggcgacca ttcggcgatg ccgggaacac
     4561 cgacactccg gccggtggcg ccatcgcgtt ttagcttctc ccagccggcg ccactgccga
     4621 gtaacaacga gccgttgttt ttgaacaaca ccggttccgg ccaatccgcc ggcagattcc
     4681 ggataaatgc cgcgccatcg agcgccaact tcgtcaggtc gggctcggcg atacattggc
     4741 gcagcatcgc cgcattgcgt cctgaagcgt gaaatcccgg aatgctctcc tgttccaaaa
     4801 ccagcagatc ggatacaccc tggcgcgcca attgataagc agtggcggcg ccggcgaaac
     4861 cggcgccgac gatgatgtaa gcaaagtttt tcatggtaac gaccgctccc taatttctat
     4921 acgaagtccg cgctatttcc aaagggtagg cgggagtttt tgcggtctgc gggtcaacgg
     4981 cggcggcgaa tttttgggtt tgaaaccagg aggaactgcg ctatagtcct ttgcaaatga
     5041 gaatccatcg ttacacccag tgggatggga cgcaggaaat tctctttccc accaccgatg
     5101 acatcatcaa ccaactttcc gatcacttgt tagaagaaga gggcgtccgc cgcgctttgc
     5161 gcgacctgat gcgccggggg tttacctcgg aagacggcca acgctcggtc aaaggcatgc
     5221 gcgatttcct gcgccaggcc gaggagaagc gtcgcgagct gctccacaag tactcccccg
     5281 actcgttcaa actgaccccg gaggagcagc aaaaactcac tgaaaagctc ggcagcctgg
     5341 ccgagaagat ggaagcctac aacgagcaga tgcgcaactt tatggagcgc atgtccggca
     5401 aatttgccga ccgcatggac gaactcagcg agaaaatgca gcaggcttac cagcgctacc
     5461 aagagctgca gcaacgcttg aagcagcaga tccaagaacg cggcatgcag ggcaagtcga
     5521 acatgagcgg cgagcagaat ccgcagtcgc tgatggacat gctcgaccgc atgagcgagc
     5581 ttttaaacaa cgaggatttt ctcaagaact tgccgagcat gatggataac gccatcaaca
     5641 acctcgaaag catgttggaa aacatgggca acatgaccga ggaacagctg cagcagatga
     5701 gcgacatgat gaatcagatg cagcagttgg aacagttgat gaaccagttc ccgttccaag
     5761 gatctcaacg catgggcatg ggcgaggccg gacaggtgtt gagccaatta cgcggcctgg
     5821 aacgctttct gcgctggggc cagcgcggca tgggcgatcc atcggatttg aatctcgacg
     5881 agctgcgcga catcctcggc gaagaagctt acgagaatct aaaatatttg cagaatgtcg
     5941 agcagatgct cgaggaggaa ggctacatcg ttcgcggcgg ccacggccta aagctgaccg
     6001 ccaagggcat gcgcaagatc ggcgataagg cacttcgtga aatctttcag atgctcaaca
     6061 aaagccgctg gggcaatcat gacacttccg cgcgcggctc ccagggcgag cacatggaag
     6121 aaaccaagct ctacgaatac ggcgatccga tgaacatcaa tatcggcgag accgtgctca
     6181 acgcgttagg caagcgcaag cccggcgagc cgctgcgaat ccacccggaa gatttttccg
     6241 tgcagcgggt ccagtattcg accgtcgccg agaccgcgtt gttgatcgac gtgagctact
     6301 cgatgctgat gaacgatgcg ctccacgccg gcaagaaagt tgccctggcg ctgcatcgct
     6361 tgatccagac gaaatatccc caagacacgc tccacctggt ggcatttcgt tccaatgcca
     6421 agttgattca agccgaagat ctgccgtcca tcgtttcgct gacttatttc atggagcatg
     6481 gcacggacat caaagaagcc ttgcgctacg cgcgtcagtt attgggctcc aatcgcagtg
     6541 ccaatcggca gattatattg atcaccgacg gcgagccgac cgcggcaacc ctcgaccgcg
     6601 gcggccggct gcacagcggc tggggctcgg cgatgctgca tggccgcatc gtccacgaga
     6661 cgctaaaaga agtgaaacgc tgcacccaga gcggtatcaa gatcaatacc ttcatgctcg
     6721 gcgccgatta ctaccgccag ggctttgtcg accagttatc acgtttgaac attggtcggg
     6781 ttttctacac cacgccggat cagatgggca actacatcat ggtcgactat ctcaacaaca
     6841 agaaaaaacg cgtcggccgc taatcgcgaa gatttgcgaa tggccaattt cagaccgcaa
     6901 attccaaact gctactaaga cctgcaatcc tagctgtttt tgaaccttga acggattatt
     6961 ccgtcaccag tacgagcacc ccggtgtaag cgaataggtg attggcccgc cccagcggcg
     7021 cgagctcgaa gccgccgaac atgccgatca acggaaaatc gcccagcgcc tgcttgatat
     7081 aggcggcgtc gatgccgggg atgccgtaca acgagctgcc gcgggcgcag caattgaagt
     7141 agaagccgaa ggcgggcgtc ttgccctcca ggcgcgctaa ttggcgctgt aacatctctt
     7201 gcaagtcgtc gcgggcgcgc tggccgtcgc gcagggtgaa aatcatcgac tgcccctcgc
     7261 tcacctggtc agccaccgcg acgcttcctt cgtcgggatc caagccgaca atattacgca
     7321 caagatagtt gcccgcggcg acgctgtttt cgctgcgatc cgccggcagg ccgacgaata
     7381 agaccgttaa agcgcgccgc aaatcttcgg ccagcggccc cttgagcagc ttggcgaaca
     7441 caggcagggc cggctggttg tcgatctcga gtatcacatt atttttcgct ttggtgatcg
     7501 tcatcggcgg ggtgatcggc tggcagcctt gcgtgatatc gatatgagtc tcgaacgcgc
     7561 cggagatata cataccggcg agcgcgttgg tagtcagctt gtcgccgcat aactgataag
     7621 tcgcgcccgc cgcgccgttc tccgacgatc ccgcgccggc gatcggatga aagccggcgc
     7681 gcgctgcgat cgcctgcaag aggttttgtg cgttgccgtt gtaggtgtcg ggaaacagca
     7741 gcatcagcga tttctcatcc tgcgccttgg cgagaaagcc gccgaaggtt tcgcctaggt
     7801 ttgcttcgtt accgcgcaac ggttcgaaga taaacggccg ggcaaaaaac cgatcgctgg
     7861 caaacaccat cacggcaacg ccgtggtcgc cttcgatctc gccggctccg gtcaataccc
     7921 ccgcgccact cgaaccggcg acacaatcgg tgccgatggt ccgcg
//
LOCUS       DASQKS010000518         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3234371, whole genome shotgun sequence.
ACCESSION   DASQKS010000518 DASQKS010000000
VERSION     DASQKS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3234371"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1120)
                     /locus_tag="VE170_13320"
     CDS             complement(137..1120)
                     /locus_tag="VE170_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16468.1"
                     /translation="MNPRHWSLFVLSLIVALNWAQTSSAAEPKLLNVGWTGGTAWTAL
                     PDRVAMERGFFEKEGLRPRYVQFQGTNLMLSALMANELDYVTILPFIAGAATRGLPVK
                     IVATTVKTSGYAIISRPDIDSVKALKGKRIAINTFGSSADFAIYQLLSRNGLDPNKDV
                     TLQAIAGSPDARFAALLSGAVDATVVNSPFEYRAEQRGFKTLLSVKETAEFVKIPIVG
                     LSTTQRKIDREPDEIVRYLRALRNAILFLQSQREAGVSLIEKLLKLDRPVAERFYGIY
                     RDQYNPDLSVPDSVVEEWIAVGTFRAKEKIIAKPSVVVDWSFAEKARTSQR"
     gene            complement(1128..1865)
                     /locus_tag="VE170_13325"
     CDS             complement(1128..1865)
                     /locus_tag="VE170_13325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ16469.1"
                     /translation="MKTSWEKVTVDNSPMGMYLAQPEGAGAVPAIIVVQNQDGVAEFT
                     QEMTRRVAAAGYVGIAPQFYHREGEPKTPEATASIKTSRNDANVMNDIAATVSFLKGC
                     ATVDSSRLGIVGFCMGGRIAFLGAAATTLFKVAVDFYGGGCYSQWGDRPAPATMAAQV
                     CCPVQGHFGELDKNPPAEEMRRLDAELTRLGKPHEFFFYPDTHHGFNRSGGKTYKPDH
                     DATSWARTLEFFAKHLAATEQKKAVAG"
     gene            2043..3530
                     /locus_tag="VE170_13330"
     CDS             2043..3530
                     /locus_tag="VE170_13330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYJ16470.1"
                     /translation="MNARGWGLLFRRRAAWAAVGVASVFFATAVIVKAGEAKSAKRRA
                     VTAKAAAKAPTVAANSGAAAKPSAAGKSYDIVFNEFKLTNGLRVLLAEDHRAPTFSIC
                     MTYNVGSRDERPGRTGFAHLFEHMLFQGSENVGKGEHFILVQNNGGSANGTTNADRTN
                     YFETLPANQLELGIFLEADRMRAPAINQANFDNQRSTVQEERRQNYDNRAYGKTYEAV
                     IDLVYDNFPYKHSTIGSMDDLNAATLDDATGFFRTYYAPNNAVLSLVGSFKTEAALAL
                     IKKYFENIPPQPAPAPADMSEPEQKAERRKTIEDGFAQTPRLDIVYKIPPANTPDWFA
                     LEILGQVLSSGVSSRLYQKFVKEKEMAVSVYADAEEKRGPSLFWFSLMARPTTDLGAL
                     EKLLYEEIARIQKSPVEDSELNKVRMQLRRQRAQQLYSTRSRANSLGHFAVYYNDPSL
                     INKIWARYEQITKADIQRVAQVYFTDSNRAVVTTLPKTAAAPSGS"
BASE COUNT          745 a         1013 c         1081 g          752 t
ORIGIN      
        1 gaagtcgacc ggcagaggtt ggacatggat ccttccagcg gcgaagagat ccaagcgctg
       61 cacagagatt tactggaaca aaaaggcgaa gtcatcgagc gggtcaagaa gattttgcag
      121 aattgatctc tctccgttaa cgctggctag tgcgcgcctt ttcagcgaag ctccaatcga
      181 ccaccaccga aggtttggcg atgatttttt ctttggcgcg aaaggtgccg acggcgatcc
      241 attcttcgac gaccgagtcg ggcacggaga ggtcggggtt gtactggtcg cgataaatgc
      301 cgtagaagcg ctcggcgacg gggcggtcga gcttgagcaa tttttcgatc aagctgacac
      361 cggcttcgcg ctggctttgc aaaaacagga tggcgttgcg cagggcgcgc agataacgga
      421 cgatctcatc cggttctctg tcgatctttc tctgcgtcgt gctcaagccg acgatcggga
      481 tcttgacgaa ctcggcggtt tccttcaccg ataaaagcgt cttaaagcct ctttgctcgg
      541 cgcggtactc gaacggactg ttgaccacgg tggcgtcgac ggcgccgctc agcagcgcgg
      601 caaaacgagc gtccgggctg ccggcgatgg cctgcaaggt gacgtccttg ttgggatcga
      661 ggccgttgcg gctgagcagt tgatagatcg cgaagtcggc cgagctgcca aatgtgttga
      721 tggcgattct cttgcccttg agcgctttga cgctgtcgat atcgggccgg gaaataatcg
      781 cataaccgct ggtcttgacg gtggtggcaa cgattttcac cggcagaccg cgggtggccg
      841 cgccggcgat gaaaggcagg atcgtcacat aatcgagctc gttggccatc aaggcgctga
      901 gcatcaggtt ggtgccctgg aactgcacgt agcgcggcct gagcccttct ttttcgaaga
      961 agccgcgctc catggcgacc cggtccggca gcgccgtcca ggcggtgccg ccggtccagc
     1021 cgacgttgag cagcttgggc tcggcggctg aagaggtttg tgcccagttc agcgctacga
     1081 tcagcgacag gacgaagagt gaccagtgcc tggggttcat tctttgccta accggcgact
     1141 gcctttttct gttctgttgc ggcaagatgc ttggcgaaaa attccagcgt tcgagcccag
     1201 gatgtcgcat catggtcggg cttataggtc ttcccaccgc tgcgattaaa accgtgatgc
     1261 gtgtcgggat aaaagaaaaa ttcatggggt ttgccgaggc gggtgagctc ggcgtcgagc
     1321 cggcgcattt cctccgccgg cggatttttg tcgagctcgc cgaagtggcc ttggaccggg
     1381 cagcaaacct gcgctgccat ggtggccggc gccggccggt caccccattg cgagtaacag
     1441 ccgccgccgt agaaatcgac ggcgactttg aagagcgtgg tcgctgccgc gccgagaaag
     1501 gcgatgcgtc cgcccatgca gaagccgacg atgcccaagc gtgagctatc caccgtggcg
     1561 cagcccttga gaaaactaac cgtcgccgcg atgtcgttca tgacgttggc gtcgttgcgg
     1621 ctagtcttaa tactcgccgt tgcctccggg gtcttgggtt cgccctcgcg atgataaaat
     1681 tgcggcgcga tgccgacgta accggcggcg gccacccgcc gggtcatttc ttgggtaaac
     1741 tcggcgacgc cgtcttgatt ctgcacgacg ataatcgccg gcacagcgcc agcgccttcg
     1801 ggttgggcga ggtacatgcc catcgggcta ttatcgacag tgactttttc ccaggatgtt
     1861 ttcatacaat ctttggcctc agcctctcta caaattttgt cttcatttat gcttcccatg
     1921 ggcgtgctgt caaacgctgc gcgagctaac ggggtgcggc ggctccccgg ataatttcct
     1981 tgtcgtcgga atgatgttac tactaagccc atggccagag tcgctaaacg ggagagtttg
     2041 caatgaacgc taggggttgg ggtttgcttt ttcgacgcag ggccgcctgg gcggcggttg
     2101 gggtggcgtc ggtgtttttc gccaccgccg taattgttaa agccggcgag gctaagtcgg
     2161 ctaagcgccg ggcggtgacc gccaaagcag cggccaaagc accgaccgtt gcggcaaatt
     2221 ccggcgccgc ggcaaaaccc agcgcggcgg ggaaatctta cgatatcgta tttaacgaat
     2281 tcaaactgac caacggcctg cgcgtgctgc tggcggagga tcaccgcgcg ccgacctttt
     2341 ccatctgcat gacttacaac gtcggctccc gcgatgagcg ccccgggcgc accggctttg
     2401 ctcatttgtt cgagcatatg ctgtttcaag gctcggaaaa tgtcggcaag ggcgagcatt
     2461 ttattctcgt gcaaaacaac ggcggcagcg ccaacggcac caccaacgcc gaccgtacca
     2521 actattttga aacgttaccg gcgaatcaat tggagttggg gatttttctc gaggccgacc
     2581 gcatgcgcgc gccggcgatc aatcaggcga acttcgacaa tcaacgctca acggtgcaag
     2641 aggaacgccg gcagaactac gataaccgcg cctacggcaa gacttacgag gcggtgatcg
     2701 atttggtcta cgataatttc ccgtataaac attcgaccat cggctccatg gacgatctca
     2761 acgccgcgac gctggacgac gccacgggtt tttttcgcac ctactacgcg ccaaacaatg
     2821 cggtgctctc gctggtcggg agtttcaaaa ccgaagcagc cttggcattg atcaagaaat
     2881 atttcgagaa tattccgccc cagccggcgc cggcgccggc tgacatgagc gagccggagc
     2941 agaaagccga gcggcgtaaa accattgaag acggcttcgc gcagacgccg cgcctcgata
     3001 tcgtctacaa aatccccccg gccaacacgc cggattggtt cgccttggaa atactcgggc
     3061 aggtgctttc cagcggcgtg tcctcgcggc tctatcagaa gtttgtcaag gaaaaagaga
     3121 tggccgtaag cgtctatgcc gacgccgagg agaagcgcgg accgtcgctg ttctggttca
     3181 gcctgatggc gcggccgacc accgacttgg gtgccctgga aaaactgctg tacgaagaga
     3241 tcgcgcgcat tcaaaaaagc ccggtggaag attcggagtt aaataaagtc cgcatgcagt
     3301 tgcgccgcca acgggcgcag cagctctaca gcacgcggtc gcgggctaac tcgctaggcc
     3361 atttcgccgt ctactacaac gatcccagtt tgatcaacaa gatttgggcg cgctatgagc
     3421 agatcaccaa agccgatatt caacgcgtcg cgcaagtata ttttaccgac tccaaccgtg
     3481 cggtggtgac caccctgcca aagacggccg cggcgccgag cggcagttga gcgaccaaca
     3541 tgagtaaatc gatggcgaaa tttttcttga ttttttatac cgtattggcc g
//
LOCUS       DASQKS010000519         6786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4889879, whole genome shotgun sequence.
ACCESSION   DASQKS010000519 DASQKS010000000
VERSION     DASQKS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4889879"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VE170_13335"
     CDS             complement(<1..142)
                     /locus_tag="VE170_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HYJ16471.1"
                     /translation="MMKLYFSPGACSLASHIGLEETGAPYEIEPIMLAKGQQRTADYL
                     KIN"
     gene            complement(453..776)
                     /locus_tag="VE170_13340"
     CDS             complement(453..776)
                     /locus_tag="VE170_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16472.1"
                     /translation="MQNGAAAEKVEAALGDYKKNPLFSLRERLALELAERMTYTSKRV
                     SDKFFKRLKKYFTDEELVELAAVIALENFRSKFNPVFGVEANNFCPLPGIKEAAAAAM
                     RRFHE"
     gene            complement(801..1043)
                     /locus_tag="VE170_13345"
     CDS             complement(801..1043)
                     /locus_tag="VE170_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16473.1"
                     /translation="MARIKGITESDANDEVRQIFAEQKKRHGFVSNAAQVSAVRPTIQ
                     KGVQALAQGIQDSGLIAQELRNLVCVRTAHINGCPY"
     gene            complement(1254..1661)
                     /locus_tag="VE170_13350"
     CDS             complement(1254..1661)
                     /locus_tag="VE170_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ16474.1"
                     /translation="MEWREALPLLQGNHTGVAATVTPKGRAQATIVSTAVLDGKVGVA
                     ARPRTVKAKNIERTGRAAITVIKLENRRYVTVEGPASIEPWQDTPAHIKRLKDFYVSM
                     GRAKGTDEEFAKQMREEQRSLILVTPEQLYGSV"
     gene            1948..3129
                     /locus_tag="VE170_13355"
     CDS             1948..3129
                     /locus_tag="VE170_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYJ16475.1"
                     /translation="MITGALEGFTCIDVSGHVAGPYAGSLLGDLGCEVIKVELPKGGD
                     THRGRNPKYDGYGPSFRALNRNKKSVTLNLRDKKAREILHKLLATADIFLENFRPPTR
                     QQLGLDYDELAKLNPKLIHCSISGYGQSGPYRDKPGFDTIGQALSGMMSLVTDLDDPK
                     VAGISFVDHSAGIFAAHGIMAALLARSKTDRGQFIDVSLLQVAIAFIESHVSDYLNGG
                     EAVSRDNFPKGRIYSLLDCDKKPFMVHLSGHNEAWEGLLKAGELDERVGDPRFATRKQ
                     RSDRHDEIVAMLRAAFVRHRRDYWLPRLDANSIPNAPINTIQEVFDNPQIKHLGIPRQ
                     IEHPRMGVSNLVGSPINMSGTPPKFFRAAPLLGENTEEVLAKLGYDRNAVKDFKASGV
                     I"
     gene            3220..4434
                     /locus_tag="VE170_13360"
     CDS             3220..4434
                     /locus_tag="VE170_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16476.1"
                     /translation="MDFNVSADPPHVVEFRKEVGAWLAEQFKGNEHLRWSASWSTRED
                     ADEYQFRRGLAEKLGAKGWLFPLFPKQYGGAELTVDHQMVLETELFKYGLNLSHTFYT
                     LARIVAPVLIRWGTEEQKAEFLPAITKAQICVWQVLTEPHGGSDIANCQTKAIRDGDH
                     YVVNGQKVMVGHHLPPDFLWTLVCTNPQGKRHENLAWLHIPANLPGITIQHMNLMMGI
                     KNAVFFDNVRVPAKYLIGGENNGWKVASTHMELEHGGDGRIAVDPLIERVVEYCRNTH
                     IDGKPIIEDPRVRDLVADMTIELNTLRRFNQLIYWCRFAKKPHPYGGPQQRYFQRMLR
                     LRNGERLQAIMGADALVQNLSVHEVIDFEYAVRTGPGQLHGGGTLDTDRLIFARRLGL
                     GRPTKETAPETH"
     gene            4556..5677
                     /locus_tag="VE170_13365"
     CDS             4556..5677
                     /locus_tag="VE170_13365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16477.1"
                     /translation="MDLGLTEDQEMLKKVAADFVKAEVPAHQMTQWYKNKQVFRPEIL
                     KKAAELGWLGMMLPEEWGGAGLSPMDCAVVFEQLGHGPVPGPLFTSGILSAQMIFEAC
                     TDAQRKALLPELCSGKTIVVPAIVDQSAYWGPEAVETRLSKTPSGYLINGTKRFVFDA
                     DAATSFLCAARSEEGKVVFLLVDAKSPGVTVTQHIGFFVSVFEVRFNNVTVSADNLLG
                     SSGASWATLENALDKALPILSAYQVGATQEVFDFTCEYTRTRVVFGQPIGRFQRVQDH
                     CVDISICLDTARWATYEAIWRLETGMDARAGVHEAKATASDGYYHGTNYAHMVWAGPG
                     TDYSHPMMAHSVLAHTLYQYLGTPGHHKRLMVDALYPRA"
     gene            5772..6776
                     /locus_tag="VE170_13370"
     CDS             5772..6776
                     /locus_tag="VE170_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16478.1"
                     /translation="MKYYLLLVTAVSLSFSLAANLDAQSQSSLIDGAKKEGKLTWYTS
                     MAVDTSKPVFDAFTKEYPFIKPELVRLGEEQLVNRILSETRAGRWAFDVLSTSSIATM
                     VERKMITPYLAPDRDLFMDQFKDPQGYWTGVFVNNLVLAYNTKMLAAKDAPKSYEDLL
                     APKWKGQMLMDSTDYDWFGTLLTVWGRERTVKYMQQLAKQDPLWRRGHGLTAQLVGAG
                     EVPLAWAYNFRIERMKSDGAPVDWIESFDPIVATISGIALSAKATNPNAAKLLINFAT
                     SRKGQEMIREMRRIPARTDVKPLAVKMDQSKLKLKAVPREVYTGLDDHAREFRKIFGM
                     "
BASE COUNT         1507 a         2008 c         1860 g         1411 t
ORIGIN      
        1 ggttgatctt caaatagtcc gcggtcctct gctgcccctt ggccagcatg attggctcga
       61 tctcgtacgg cgcgccggtt tcttcgagac cgatatgcga agccaaggaa cacgcgccag
      121 ggctgaaata aagcttcatc attggaatac ctccggtttt gtcaaattca atactacgtc
      181 gcagcgcctg acccagcagc cgagcgggcg ccacgccatg ttttcggaca gtaagcgcta
      241 acgctggaat cctgtcaaga tgacatcggc ccgcccgagg gtcgagaatc agtttaataa
      301 attattttat cgaatcgcta acggccttct gatttcaata cgtatcggaa tgatgatcgg
      361 gaaacattgc cggcgagtaa ttcttgcccg actgatgcaa ggagaatggc ttgctggcag
      421 aacgatctac cgcaccggca tagcgcgtcg gctcactcat gaaagcgccg catggcggcg
      481 gcggcggctt ctttgattcc gggcagaggg cagaaattgt tcgcctcgac accgaagacc
      541 ggattgaact tgctgcgaaa gttctcgagc gcgatcaccg cagctagttc gactaattcc
      601 tcgtcggtaa aatacttctt gagacgcttg aaaaacttat ccgagacccg cttactcgtg
      661 taggtcatgc gctcggccag ttccaacgcc agccgctcgc gcaaggaaaa caatggattt
      721 ttcttgtaat ctcccaatgc cgcttcaacc ttttccgccg ccgcgccgtt ttgcatcgcc
      781 ccggatgcat tactatcaat tcaataggga cagccattga tatgggcggt cctgacgcag
      841 acgaggttgc gtaactcctg cgcgatcagt cctgaatcct gaatgccctg ggcgagcgcc
      901 tgaactcctt tctgaattgt cggcctgacg gcggacacct gcgccgcgtt tgagacaaac
      961 ccatgacgct tcttctgctc tgcgaaaatc tgccgcacct catcattggc atcactttcc
     1021 gttatccctt tgatcctggc catgaatttc ccctccgtta ccttcgatgc agagactacg
     1081 agacgacgcc cgcattctgt tcatgcactc tgcgatcttt gctagaaatc cgctccccgg
     1141 tgaccgagcc ctgtgcgctc agcaatgttg ccagctaatc cgcttgcagt cccaaatgcg
     1201 ctatcaactt cgacgcggtc gcggggcgga agggaaacgc gtcacgcgcg tgtttaaacg
     1261 ctgccgtaga gctgctcagg ggtaaccaga atgagacttc gctgttcctc gcgcatctgc
     1321 ttggcaaatt cctcgtcagt ccccttggct cgtcccatcg aaacgtaaaa gtccttcagg
     1381 cgcttgatgt gcgccggtgt gtcctgccaa ggttcgatgg acgccggccc ttcgaccgtg
     1441 acgtaccgtc ggttttcgag ttttatgacc gtgatagccg ctcggcccgt ccgttcgatg
     1501 tttttagcct tgacggtgcg cggccgcgcc gcgacgccga ccttgccgtc cagcaccgca
     1561 gtcgaaacga tggtcgcctg tgcacgccct ttcggcgtga cagttgcagc gacacctgta
     1621 tgatttcctt gcaataaggg aagcgcttct ctccattcca tttgatcgtt cctccgaaag
     1681 ttgaagtatt ggcggctcga ccgcgatcaa ctcgcagcta taaccctgcc cacagcgatg
     1741 agtcaagccg caatggcgtc ggaccgcgca ctttatctgt ccgcgtcttg ttggtcgcgg
     1801 gttagaaaac acgccgttca tcgggatcga cctcacattt tgaacgactt gaacgattta
     1861 aaccatttga accccggtgc ccgtttcgcg aggtggaaaa gcttttttga ttgcgataag
     1921 tgaaatgctt gaggttttga atcacgcatg atcaccggcg ctctcgaagg atttacttgt
     1981 atcgatgtca gcggccatgt tgccgggccc tacgccggca gcttactcgg cgacttgggc
     2041 tgtgaagtga tcaaagtcga gctgcccaaa ggcggcgaca cccaccgcgg gcgcaatccc
     2101 aaatatgacg gttacggccc gagctttcgc gcgctgaatc gaaataaaaa aagcgtcaca
     2161 ctcaacctgc gtgacaaaaa ggcccgtgag attttgcaca agctgctcgc cacggcggat
     2221 atttttctgg aaaacttccg tccgccgacg cgccagcagt tgggcctcga ctacgatgag
     2281 ctggcgaaat tgaatcccaa actgattcat tgctcgatct ccggctacgg ccagagcggg
     2341 ccgtaccggg acaaacccgg cttcgacacc atcggccagg cgttgagcgg catgatgagt
     2401 ctcgtcaccg acctcgacga tcccaaagtc gccggcattt ccttcgtcga ccactcagcc
     2461 ggcatcttcg ccgcccatgg catcatggcg gcgctgcttg cccgcagcaa aaccgatcgg
     2521 ggccagttca tcgatgtctc gctcctgcaa gtcgccatcg cgtttatcga gtcccatgtt
     2581 tcggattact tgaacggcgg cgaagccgtg tcgcgggata actttcccaa ggggcgaatt
     2641 tacagcctgc tcgattgtga taagaaaccg ttcatggtgc atttgtccgg ccacaacgaa
     2701 gcttgggagg gtctgctcaa agccggcgag ctcgacgagc gggtcggcga cccgcgcttc
     2761 gccacgcgca agcaacgcag cgatcgccat gacgagatcg tcgccatgct gcgcgcagcg
     2821 ttcgtcaggc accgacgcga ctattggctg ccgcgccttg acgccaatag catccccaac
     2881 gcgccgatca acacgattca agaagtgttc gacaacccgc agatcaaaca tctgggcatt
     2941 ccccgacaaa tcgagcatcc gaggatgggc gtgagcaatc tggtcggcag tccgatcaat
     3001 atgtcgggca cgccgccaaa attttttcgc gccgcgccgc tgcttggaga aaacaccgaa
     3061 gaagtattgg cgaagttggg ttacgaccgc aatgctgtta aggattttaa ggcaagtgga
     3121 gtgatttgaa tttcttcggg agtcgagcgc ctggcgtgca acattaaccc cagagcgaga
     3181 cgcccaattc tagactcaca ggcgatgaag gagtgaacca tggactttaa tgtcagtgca
     3241 gatccacccc acgtagtgga gtttcgcaaa gaagtcggcg cctggctcgc cgagcagttc
     3301 aaaggcaacg agcatctgcg ttggtcggct tcctggtcga cgcgcgagga tgccgacgag
     3361 taccagttcc gccgcggcct ggccgaaaag ctcggcgcca agggctggtt attcccgctt
     3421 tttcccaaac aatacggcgg cgccgaactc accgtcgatc atcagatggt gctggaaacc
     3481 gagctgttca aatacgggct taatctctcg cacacgtttt acaccttggc gcgcatcgtc
     3541 gcaccggtgt tgatccgctg gggcacggaa gaacaaaagg cggaattctt gccggccatc
     3601 accaaggcgc agatctgcgt ctggcaggtg ctcaccgagc cgcacggcgg cagcgacatc
     3661 gccaattgtc agaccaaagc gatccgcgac ggcgatcact acgtcgtcaa cggccagaaa
     3721 gtcatggtcg gccatcatct gccgccggat tttctctgga cattggtgtg cacgaacccc
     3781 cagggcaaac gccacgaaaa cctcgcctgg ctgcatatcc cggccaacct tccgggcatc
     3841 acgattcaac atatgaactt gatgatgggc atcaagaacg ccgtgttttt cgataacgtg
     3901 cgtgtgccgg caaaatactt gatcggcggt gagaacaacg gctggaaggt cgccagcacc
     3961 catatggagt tggaacatgg cggcgacggc cgcatcgccg tcgacccgtt gatcgaacgc
     4021 gtcgtcgaat actgccgcaa tactcacatc gacggcaaac cgatcatcga agatccgcgc
     4081 gtgcgcgacc tggtcgccga catgaccatc gagttgaaca ccctgcgccg cttcaatcaa
     4141 ttgatctact ggtgccgatt cgccaaaaag ccgcatccct acggcggccc ccaacagcgc
     4201 tattttcaac gcatgctgcg cctgcgcaac ggcgaacgct tgcaggcgat catgggcgcg
     4261 gacgccctgg tgcaaaatct atcggtgcac gaagtgattg acttcgagta cgctgtgcgc
     4321 actggccccg gccaattgca tggcggcggc acattagaca ccgaccggct gatcttcgcc
     4381 cgtcgtctcg gcctcggccg tccgaccaaa gaaaccgcgc cggaaacgca ttaattggcc
     4441 atggggcaac aggtaacaac tggaacatcc ggaaccattg gaacgattga tacatgagct
     4501 gtttcgttcc agaggttcca taaggttcca cgggctctga acgtaggaga aagaaatgga
     4561 tttaggttta accgaagacc aagagatgtt gaaaaaggtt gccgcggatt tcgtcaaggc
     4621 agaagtgccg gcgcaccaga tgacccagtg gtacaaaaac aagcaagtgt ttagaccaga
     4681 gatcttaaaa aaagccgccg agctcggctg gttgggcatg atgttgccgg aagaatgggg
     4741 cggcgccggc ctgtcgccca tggactgcgc cgtggttttt gaacagttgg gccacggccc
     4801 ggtgccgggt ccgctgttca cgtccggcat actcagcgcc cagatgattt ttgaagcctg
     4861 caccgacgca caaaggaaag cgttgttgcc tgagctgtgc agcggcaaga ccatcgtcgt
     4921 accggcgatc gtcgaccagt cggcctactg gggaccggaa gcggttgaaa cccgcttgag
     4981 caaaaccccc agcggctatt tgatcaacgg caccaagcgg ttcgtcttcg atgccgacgc
     5041 tgcgacgagc tttctctgcg ccgcgcgcag tgaagaaggc aaagtggttt tcctgctggt
     5101 cgacgccaag agccccggtg ttaccgtgac ccagcacatt ggcttctttg tctcggtatt
     5161 tgaagtgcgt ttcaacaatg tcaccgtcag cgccgacaac ctgctcggct cgagcggcgc
     5221 cagttgggcg accctggaaa atgctctcga caaagcgctg ccgattttgt ccgcctatca
     5281 agtcggcgcc acccaggaag tctttgactt cacctgcgag tacacccgca cccgcgtggt
     5341 cttcggacaa cccatcggcc gcttccaacg ggtccaagat cactgcgtcg atatttcgat
     5401 ctgcttggac acggcgcgct gggccaccta tgaagcgatt tggcgccttg aaaccggcat
     5461 ggacgcccgc gccggcgtgc atgaagccaa agccaccgcc agcgacggct actatcatgg
     5521 caccaactac gcgcatatgg tctgggccgg gccgggcacg gattacagcc acccgatgat
     5581 ggcgcacagc gtcctcgctc atacgctata tcaatatctc ggcacgcccg gtcatcataa
     5641 acgcttgatg gtcgacgcgc tttacccgcg cgcgtagaac tgatcgagga tggacggtag
     5701 aagatcgggt gcttcaatcc gagccgtcct ccatcgccca tcctcgaccc tgagcagccc
     5761 ggtggagtag catgaagtat tatttgcttt tagttactgc ggtaagcctc agtttctcgc
     5821 tcgccgccaa cctcgacgca caatcccagt ccagcctcat cgacggcgcc aagaaagaag
     5881 gcaaactcac ttggtacact tcaatggcgg tggatacctc gaagccggtg ttcgacgcct
     5941 ttactaaaga atacccattc atcaaaccgg aactggttcg attgggggaa gaacaactgg
     6001 taaatcgcat tttgagcgag acccgcgccg ggcgctgggc atttgatgtc ctatcgacaa
     6061 gctcgatcgc gacgatggtc gagcgcaaga tgatcacgcc ttatctagcg cccgatcgcg
     6121 atctctttat ggatcaattt aaagatcccc agggctactg gaccggcgtg ttcgtcaata
     6181 atctggtgct cgcttacaac accaagatgc tcgccgccaa agacgcgccg aaaagttatg
     6241 aggatctgct cgcgcctaaa tggaaagggc agatgctcat ggactccacc gactacgact
     6301 ggttcggcac gttgttgaca gtgtggggcc gggaacggac cgtcaaatat atgcagcagc
     6361 tggccaagca agacccgctc tggcgccgcg gccacggctt aaccgcgcaa ctcgtcggcg
     6421 ccggcgaagt acccttggcg tgggcctata acttccgcat cgaaagaatg aagagcgacg
     6481 gcgcaccggt ggattggatc gaaagcttcg atccgatcgt cgcaaccatc agcggcatag
     6541 cgctcagcgc caaggcgacc aaccccaatg ccgccaagct cctgatcaac ttcgcgacct
     6601 cgcgtaaggg ccaggaaatg atccgtgaga tgcgtcgcat cccggctcgg accgacgtca
     6661 aaccgctggc ggtgaaaatg gatcaaagca aattgaagtt gaaagccgtg cccagagagg
     6721 tgtacacagg cctggacgat cacgctcgcg agtttcgtaa aatcttcggc atgtaacgac
     6781 ccggca
//
LOCUS       DASQKS010000520         8775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3235882, whole genome shotgun sequence.
ACCESSION   DASQKS010000520 DASQKS010000000
VERSION     DASQKS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235882"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..1040
                     /locus_tag="VE170_13375"
     CDS             525..1040
                     /locus_tag="VE170_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16479.1"
                     /translation="MFGEIWIEPYRKLLPNWSYVALLDERVIGYLTGCPDTASFVRRE
                     FVRCTLPLLRQIVVGRFRGDSYGQRFARQQLGLEKTVARCFPRPVRRQIAAAFPAHLH
                     MNVDSDYRHGGVGTRLLTHYVDDLRRVRIPGVHLFCGGTPVPFYTRVGFCELAVTQVR
                     GHDVHAMTLSL"
     gene            1120..2511
                     /locus_tag="VE170_13380"
     CDS             1120..2511
                     /locus_tag="VE170_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYJ16480.1"
                     /translation="MADATKKKFRLSLKLKLSVLITLLIVLSVVLVGDYLLDQAKESL
                     RGEITKRGRTIAQQLAASAKNPLVTRDELTLSLLVRDALRDPDVAYVIAVDEDNQIVA
                     HGELNLIGKKYQRPASLKPLANDMLVQSYDDTAGGEIIDFAVPLEFSKVRVGALYLGF
                     SDRSIRQTIERARGQTIWITGLMIVGGVVGAIGLSIVLTRPLLTLAQGTKTIAAGSFD
                     VVLPVTSRDEIGDLTVSFNEMAKNLKEKEKIKHAFSSYVARQVVDEILKNPGGIMLTG
                     ERRNVTVIFCDLRGFTPLAEKLPPEEVVAALNDFYSLMIETTIKNDGSINKFLGDAVM
                     AIFGAPVYFADHSLRAVKTAVGMQTQVAALSALRVSNGKPPIAIGIGVSAGEAVAGTV
                     GTESQMEYTVIGDRVNLAARLESNAKPGQILISQETYEQVADHVKARCLGPMTVKGRE
                     EAVTVFEILGMAD"
     gene            2542..3543
                     /locus_tag="VE170_13385"
     CDS             2542..3543
                     /locus_tag="VE170_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ16481.1"
                     /translation="MKTLGLLILCLTLAGCGAATSTPPPVHTDQPAGEFFESEDYFVV
                     VAKSGDTTQTLAAKHLGDANKDWMIEEYNETATLMPGQAVAIPKRPWNLSGVTLTGYQ
                     LVPILVYHDLAPQAKGRMVMGVKGFEEQMRYLKANGYRVITLKEYVEFVSLKRQLPKK
                     SVLITFDDGYRSFVRYAYPVLKELGFSATLFIYTDYVAAGGNAFTWPELKKLSDEGFA
                     IQAHSKSHGDMVRANAEGAAEYDKRLESELVQPKALFQKNLGYAPDIIAYPYGRQDDA
                     VVRRTKERGYVAAFTVRRQGSQSFVDPHRIHRIQIYPEMRMDDFVKSLSTFSQETIK"
     gene            3540..4664
                     /locus_tag="VE170_13390"
     CDS             3540..4664
                     /locus_tag="VE170_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYJ16482.1"
                     /translation="MKKRRQTQFFLLWAGMALFAGCSARSGDSVPANFESLIAPHRAR
                     AAALAEHGNLRGASEAWKVALTIDPRDAVALAESRKVDEQIKQSVGDRLSRGRDALKR
                     NVHLEARNHFLAVLAMDPGNREAFEALQSQVREVRQVNHTVRAGDSLVSIAQLYYGDR
                     TRSEVIWETNQLPPNPKLTPGMVLKIPEIPGLPFGRPEPAPIVKTPPSGSGPSTAKTE
                     PAEEGLYANPALGAAREAFERGEFVLALSTVDRFILQNPRNGEATDLRRIVLLQQGRT
                     LMEQNKLLDAYSAANQLVKTNPKDPAATALLSQARGRLVQQHYNQGMQLFREEKLPAA
                     VAEWRTVLQYDPAHDGAKRNIDQAQRLLKGLEERQQKQGK"
     gene            complement(5030..6235)
                     /gene="ilvA"
                     /locus_tag="VE170_13395"
     CDS             complement(5030..6235)
                     /gene="ilvA"
                     /locus_tag="VE170_13395"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HYJ16483.1"
                     /translation="MITFVDVEEARERIKEHIYFSPFPYSETISRMTGNRVFFKLENL
                     QLTGSFKERGALNRLLTLTAEEARRGVIAASAGNHGMALAFHSRRLNIAATIVMPVIA
                     PLTKVSRVRQYGAQSVLHGDDYDSALAEARRLSQERGMTFVSAFNDPWIVAGQGTIGL
                     ELYGQNPDLDAVVVPVGGGGLIAGIALALRTLNPKIKIIGAQAEAVPSMKSAVADGAP
                     VLLPPATTIADGIAVRRVGELPFALVKEFVDEIVTVSEGEIANAVLLLLEIEKTVAEG
                     AAAVPLAALMSKKISLTGKNIGLIVSGGNIDMNLISRIIEAGMIQDGRQCRLNVIVTD
                     RPGNLARLSQRIGQLGANILQIGQSRGFGRIAIGETEVELVLETTGFEHIQRLRDGLG
                     QDGFRLVEN"
     gene            6441..7502
                     /locus_tag="VE170_13400"
     CDS             6441..7502
                     /locus_tag="VE170_13400"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HYJ16484.1"
                     /translation="MDGIELYRALAAAEKDEKRAAIFGKILRAEERHAQRWARLIQTG
                     GGVVPTYRRSLRVLLLGWLARRFGTERVVPIISALEARDEPGYINQSEAEGLPAEERA
                     HSRVLREMEERPAAEGDIVGREAWHMASHTGSLRAAVFGINDGLVSNFSLVMGFAGAE
                     AKPEYILLAGCAGLLAGSFSMAAGEYVSVRAQRELFEQQIAMEQQELEMSPKEEEEEL
                     ALIYQAKGIAEPDARMMARRIIENPRTAIDTLAREELGLDPSQLGSPWVAAASSFVAF
                     ILGAIVPVIPYMATRGGTALLASATLSCLALFAVGALLSIFSARGALFSGARMLAIGL
                     GASAITYGVGWLLGVSVAG"
     gene            complement(7521..>8775)
                     /locus_tag="VE170_13405"
     CDS             complement(7521..>8775)
                     /locus_tag="VE170_13405"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HYJ16485.1"
                     /translation="QYRQHIHNEGMVIVDKIMKEGSVGVMTTHAANSLSTDSAAAATA
                     MAIGCKAKIGALGVCADDSITVSAMEIARRRGMKLGLATNSTIYDASPAAFLCHVPNR
                     KDFPAILQRYLEMAPDVLMGGGLDQFLPKGQPGSQRTDDGDAVAEFTKLGYQAVRNLP
                     QLQRAGSGKVLGLFGLSEMNFEIDRDKQNEPSVYDLTQAAIRLLHSPRGFFTFIESEN
                     MDRASHSSDIASVIHDYREFDRAVGLAYEFYRKYPRETLIIVASDHESGGLGMTLALK
                     DLSSSKGNNQAAGSLRDFKKIQGINISLQKAATLLGTDPKGEAVDKLMADHFPGFSLA
                     PEFREKIVANQFMSRTIFAEPVANALGMMVANNTQIYWQNTAHTNIPVLVAALGVGAE
                     RFKGYYDNADFGKKLKALMEGKKHE"
BASE COUNT         1862 a         2397 c         2641 g         1875 t
ORIGIN      
        1 gcgaaaaagc ctgaagcgtt gtttaacgct ttagaaatct agcgttgatg cctgatcgga
       61 taagagcgat catatcgtgc gtgtgaattg ttggagttga gagactttaa cttgtgcgtg
      121 aagcaaataa gatggaaata ggatagcaat ctagaattga ttaaagtttt ttatcgattc
      181 gagcggcagt cagaaattag tgtcttcagg tagcgcaatc gtgaaaagcc ccacgaagcg
      241 ctgcgcgaga cagcgccgcg gacggcggca aaaagctaat gaagagatat gcatcggcgc
      301 agcaaagcga ggcgccatcg tcggcagtgt gcgcaagcac taaacaagac gcggcgccct
      361 caggggcaaa aacggcgaag cccttttaat ccatgggaaa gtcgatttcg ataaaaagac
      421 tttcggatga caccgacccg agcggttcgc gttgggcggc agtacgcgag ctctgttgcc
      481 gcgcagcgaa cgatggtgca gggatcgcag ccgggcgttg gcctttgttt ggcgagatct
      541 ggatcgagcc ctatcgaaaa ttgctcccga attggtccta tgtggcgttg ctcgacgaga
      601 gagtgatcgg ttatctgacc ggctgccccg ataccgcgag ctttgtccgc cgggaatttg
      661 tccgttgcac cttgcccctc ctgcgtcaaa tagtcgttgg ccgttttcgt ggggattcct
      721 atggccagcg cttcgctcgc cagcaactcg gtctggaaaa aaccgtcgcg cggtgctttc
      781 cccggccggt gcgccggcag atcgcggcgg cattcccggc ccacctgcat atgaacgtcg
      841 actcggatta ccgccacggc ggtgtcggaa cgcggctgct aacgcactac gtcgacgatt
      901 tgcggcgagt gcgtattccc ggcgtgcatt tgttctgcgg tggaactccc gtgccattct
      961 atacccgcgt gggattttgc gaactcgcgg ttacccaggt gcgcgggcac gacgtgcacg
     1021 caatgacgtt aagtttgtaa tcgccgagga gccgatcact tgacgcgacg cgtgagcttc
     1081 aatagagtcc gtttcctgac gcaatgaccc ctcgcaacca tggctgacgc cactaagaaa
     1141 aaatttcgcc tgtcgctaaa actgaagcta agtgtgctga tcaccttgtt gatcgtgctg
     1201 tcggtggtct tggtcggcga ttatctgttg gaccaggcca aagagagtct gcgcggcgaa
     1261 attaccaaac gcggccgcac catcgcccag cagctcgccg ccagcgctaa gaatcctctg
     1321 gtcacgcggg atgagctaac gctcagtctg ctggtcaggg acgcgctcag ggatcccgat
     1381 gttgcctatg tgattgctgt cgacgaagac aatcagatcg tcgcccatgg cgagttgaat
     1441 ttgatcggga aaaaatatca gcgcccggcc tccttgaagc cgctcgccaa cgacatgctc
     1501 gtgcagagtt acgacgacac cgccggcggg gagattatcg acttcgcggt gccgttggag
     1561 tttagcaagg tgcgcgttgg cgcgctttat ctgggtttta gcgaccgctc gatccgccaa
     1621 accatcgagc gggcgcgcgg ccagacgatc tggatcaccg ggttgatgat cgtcggcggt
     1681 gtagtcggtg ccatcggtct gtcgatcgta ttgacccggc cgctgctcac actcgcccaa
     1741 gggaccaaaa ccatcgcggc gggcagtttt gacgttgttc tgccggtgac gtcacgcgat
     1801 gaaatcggcg atctcacggt gtcgtttaat gagatggcga aaaacttaaa agagaaggaa
     1861 aagatcaaac acgcgtttag cagctatgtg gcgcgccagg tagtcgacga gattttgaaa
     1921 aacccgggcg gcatcatgct caccggcgaa cgccgcaacg taaccgtgat cttttgcgat
     1981 ctgcgcggtt tcacgccatt ggcggaaaaa ctcccgcccg aagaagtcgt cgccgcgctc
     2041 aatgacttct atagcttgat gatcgaaacg acgatcaaga acgacggcag catcaataaa
     2101 ttcctcggcg acgcggtgat ggcgatcttc ggcgcgccgg tgtactttgc cgatcattcc
     2161 ctgcgcgcgg tcaaaaccgc ggtcggtatg caaacccaag tcgcggcttt gagcgcgtta
     2221 cgggtgagta atggcaagcc gcccatcgcc atcggtatcg gcgtcagcgc cggcgaagcg
     2281 gtcgccggca ccgtgggaac tgaaagccag atggagtaca cggtcatcgg tgaccgggtc
     2341 aacttggccg cgcggctgga atctaatgcc aagcctggcc agattctcat cagccaagaa
     2401 acctacgagc aggtggccga tcatgtcaaa gcgcgctgtc tggggccgat gacggtcaag
     2461 ggaagggaag aggcggtgac ggtatttgaa attctcggca tggcggatta gagattggta
     2521 aacaaataaa aacagaggtt tatgaagacg cttgggctgc tgatactgtg tttaaccctg
     2581 gccggttgtg gcgcggcaac ttcgacgccg ccgccggttc atacggacca gccggcggga
     2641 gagtttttcg aatccgagga ttattttgtt gtggtggcca aaagcggcga tacgacgcag
     2701 actctggccg ccaagcatct gggcgatgcc aacaaagatt ggatgatcga agagtataac
     2761 gagacggcga ctctgatgcc gggtcaggcc gttgcgattc ccaagcgccc gtggaatctt
     2821 tcgggtgtga cattgaccgg ctaccaattg gtgccgatcc tggtctacca cgatcttgcg
     2881 ccccaggcga agggacgcat ggtgatgggc gtcaagggct tcgaagagca gatgcgctat
     2941 ctcaaggcca acgggtaccg cgttatcacg ctcaaggagt acgtcgagtt tgtttccctg
     3001 aaacgtcagt tgcccaagaa aagcgtgcta atcaccttcg acgacggcta ccggtcgttt
     3061 gtgcgctacg cctacccggt gctcaaggag ctgggcttct cggcgacgtt gtttatctat
     3121 acggattacg tcgccgccgg cggcaacgcg tttacctggc ctgagctgaa aaaattatcg
     3181 gacgaggggt ttgccatcca ggcgcactca aagagtcatg gcgatatggt gcgcgccaat
     3241 gctgaaggcg cggcagaata tgacaagcgc ttggaatccg agctggttca gcccaaggcg
     3301 ttgttccaaa agaatctcgg ttatgctccc gacattatcg cgtatcccta cggcaggcag
     3361 gatgacgcgg tggtgcgacg gaccaaggaa cgcggttatg tcgcggcttt taccgtacgg
     3421 cgtcaaggta gccagtcatt tgtcgatccc catcgtatcc accggataca aatctatccg
     3481 gaaatgcgaa tggacgattt cgttaaaagt ctcagcactt tcagccaaga aacgatcaag
     3541 tgaaaaagcg ccgacagacg caattctttt tgctgtgggc cggcatggcg ctgttcgccg
     3601 gttgttccgc gaggtccggg gacagcgtgc cagcgaactt tgaaagtttg atcgcgccgc
     3661 accgagcgcg agcggcggcg ttggcggagc acggcaacct gcgtggcgcc agcgaggctt
     3721 ggaaagtcgc cttgaccatc gatcctcgcg atgccgtggc tttggcggaa agccgcaagg
     3781 ttgatgagca gatcaagcaa tccgtcggcg atcgcttgag ccgcggtcgc gacgcgctca
     3841 agcgcaatgt gcatctcgaa gcgcgcaatc attttctcgc cgtgctcgcc atggatcccg
     3901 gcaatcgcga ggccttcgaa gccttgcaga gccaagtgcg cgaggtgcgc caagtcaacc
     3961 ataccgtgcg tgccggcgac tcgctggtat cgatcgcgca actgtactac ggcgaccgca
     4021 cccgttccga ggtgatttgg gaaaccaatc agctgccgcc caatccgaaa ctgacgcctg
     4081 gaatggtttt gaaaatcccc gagattcccg gtctgccgtt tggacgtccg gaaccggcgc
     4141 cgatcgtgaa aactccgccg agtggaagtg gtccgtccac cgccaaaacc gaaccagcgg
     4201 aagaagggtt gtacgccaac cccgcgctgg gcgccgcgcg cgaagcgttt gagcgcggcg
     4261 agtttgtttt ggcgttgtcg acggtcgatc gctttatatt gcaaaatccg cgcaacggcg
     4321 aagccaccga tctccgccgc atcgtactgc tgcaacaggg gcgcactttg atggagcaga
     4381 acaaattgct ggatgcctac agtgccgcca accaactggt taaaaccaat ccgaaagacc
     4441 cggcagccac tgcattatta agccaggcgc ggggccgctt ggtgcagcag cactataacc
     4501 aaggcatgca gctttttcgc gaagaaaagc tccccgctgc tgtcgccgaa tggcgcaccg
     4561 ttttgcaata cgaccccgcc cacgacggtg ccaaacgcaa catcgatcaa gcccaacggt
     4621 tactcaaagg cttggaagag cggcagcaga agcaaggaaa gtagagtaat ttaaataatc
     4681 aacgagtcga ctcttttttc cgatatcgat atctccggag aatcgtcccg tgaacttggg
     4741 ctaaaccacg ggccgatttc ccgtggatcc tatggcgttc gccgcggcca tgattgcgct
     4801 gatgagggtg tggcgggctg gttttcgttt cgcccatgcg gctttgtcat cgcgcagctc
     4861 acaccgttga tcgcatctcg ttccctgacc gcgccgatta cgcgacaagc gaaaatgtgc
     4921 cgatagcgcg gtcacgatgg agaaacgtta gagttaacaa gcgtcggaaa cgaaatgtct
     4981 gccgtctcac tgccgagcta gcatttattc aatcggttcc tcccaacggt cagttctcaa
     5041 ccaagcgaaa gccgtcttgg cctaagccat cgcgcaggcg ctgaatatgt tcgaagccgg
     5101 tggtttcgag gacgagttcg acctcggtct cgccgatggc gatgcgtcca aaaccgcggc
     5161 tttggccgat ctgtaaaata ttggcgccca actggccgat gcgttggctg aggcgggcaa
     5221 gattgccagg acggtcggtg acgatcacat tcaaccggca ctggcgtcca tcctggatca
     5281 tgccggcttc gatgatacgc gagatcagat tcatgtcgat gttgccgcca gagacgatga
     5341 gcccgatgtt tttgccggta agggagattt ttttgctcat caaggcggcc aagggcactg
     5401 cggcggcgcc ttcggcgacg gtcttttcga tttccagaag caagagcacc gcgttggcga
     5461 tttcaccttc gctaacggtg acgatctcat cgacgaattc tttgaccagg gcgaacggca
     5521 gctcaccgac gcgacgtact gcgatgccgt cggcgatggt cgtggccggc ggcagcagaa
     5581 ccggcgcgcc gtcggccacg gcggatttca tcgatggcac ggcctctgcc tgggcgccga
     5641 taattttgat ctttggattc aaagtcctca gcgccaaggc aatgcccgcg atcaagccgc
     5701 cgccgcctac cggtacgacg accgcatcga gatcgggatt ttgcccatac aattccagac
     5761 cgatggtgcc ttggccggcg acgatccacg gatcgttgaa cgccgagacg aaggtcatgc
     5821 ctcgttcttg gctcaaccgc cgggcttcgg cgagagcgct gtcatagtcg tcgccgtgaa
     5881 gcacgctctg cgcgccgtat tggcgcactc gtgagacttt tgtcaacggc gcgattacag
     5941 gcatgacgat ggtggcggca atgtttagcc tgcggctgtg aaaagccaga gccatgccat
     6001 ggttgccggc gctggcggcg atcacgccgc gtcgcgcttc ttcggcagtc agggtcagca
     6061 agcggttgag cgcgccgcgc tctttgaacg aaccggtcag ctgcaaattc tccagcttga
     6121 aaaagacccg attgcccgtc atacggctga tggtttcgga ataggggaac ggcgagaaat
     6181 aaatgtgttc tttgatgcgc tcgcgtgctt cttcgacatc gacgaaggta atcatatggg
     6241 tgcaaggtta gaatgaaaag actggcgtgc gcaagtttga taaggtaaaa ggaggaagac
     6301 acgctggaca tcagcagatt gaatcgagaa ttttgcaccg gaattatcga tacaaagctt
     6361 cctgacatct catgaccgat acaaggctga attccaatca tgccgatctt caacgttttg
     6421 gtcaaaacta tttgatagaa atggacggca tcgaactcta ccgtgcgtta gcggcggcgg
     6481 agaaggatga aaagcgcgca gctatttttg gcaaaatact gcgtgccgaa gaacgccacg
     6541 cgcagcgctg ggcgcgattg attcaaaccg gcggcggcgt agtgccgact taccgccgca
     6601 gcctgcgcgt ccttctcctc ggttggctgg cgcggcgctt cggcaccgag cgcgtcgtgc
     6661 cgatcatcag cgcgctcgaa gcgcgcgatg aaccgggata catcaatcaa agcgaagccg
     6721 aaggattacc ggccgaggag cgtgcgcaca gccgggtgct gcgtgaaatg gaggaaaggc
     6781 cggccgcgga gggcgatatc gtcggacgcg aggcttggca tatggcatcg cacaccggca
     6841 gtttgcgcgc cgcggtgttt ggcatcaatg acgggctggt gtcgaatttc agtttggtga
     6901 tgggctttgc cggcgccgag gcgaaaccgg aatatattct gctagccggc tgtgctggat
     6961 tgttggccgg gtcgttctcg atggccgccg gcgaatatgt ctcggtgcga gcgcagcgcg
     7021 agctcttcga gcagcagatc gccatggagc aacaagaatt ggaaatgtcg cccaaggaag
     7081 aagaagagga actcgcgctg atttatcagg ccaaagggat tgccgaaccg gacgcgcgca
     7141 tgatggcgcg ccgtatcatc gagaatccgc gcaccgccat cgacaccctg gcgcgcgagg
     7201 agttgggact ggacccgtcg cagctcggtt caccgtgggt ggctgcggcc agctcgtttg
     7261 tggcatttat tctgggagcg atcgtgccgg taattcccta catggcgacg cgcggcggga
     7321 cggcgctctt ggccagcgcg accttaagct gcctggcgct atttgctgtc ggcgcgctgt
     7381 tgtcgatctt ctccgcgcgc ggcgcgctgt tcagcggcgc gcgcatgctc gccatcggct
     7441 tgggtgcctc ggcgattact tatggcgtgg gttggctatt gggcgtatcg gtagccggtt
     7501 aggtgagcgc gcttccaatc ctactcgtgt ttcttgccct ccatcagggc ttttaatttc
     7561 ttaccgaagt cggcgttgtc gtaatagcct ttaaagcgct cggcgccgac gccaagtgcc
     7621 gcaactaaga ctggaatatt ggtatgggcg gtattttgcc agtagatctg agtgttgttc
     7681 gcgaccatca tgcccagagc gttagccacc ggttcggcga agatcgtccg cgacataaat
     7741 tggttggcga cgatcttttc tctaaattcc ggcgccagac taaaacccgg aaagtggtcg
     7801 gccatgagtt tgtctacggc ctcgcctttg gggtcggtgc cgagtaatgt tgcggctttt
     7861 tgcaatgaaa tgttgatccc ttggattttc ttgaaatcac ggaggctccc cgcggcttgg
     7921 ttgttgcctt tgctgcttga caggtctttt agcgccagag tcatgccgag cccgcctgat
     7981 tcatgatcgg aggcaacgat aatcagcgtc tcgcgcgggt atttgcgata aaattcatac
     8041 gctagtccta cggcccggtc gaactcacgg tagtcgtgta ttaccgatgc tatgtcactc
     8101 gaatgggagg cacgatccat attctccgat tcgataaagg tgaaaaagcc gcgcgggcta
     8161 tgcagcaggc gaatcgctgc ctgggtgaga tcatagaccg acggttcatt ttgcttgtct
     8221 cgatcgatct cgaagttcat ctcggaaagt ccgaacaacc caagaacctt gccgctgccg
     8281 gcccgctgca actggggcaa attccgcacc gcctgataac cgagtttggt gaattcggcc
     8341 actgcgtcgc cgtcgtcggt gcgttggctg ccgggttgcc ccttgggcaa aaattgatcc
     8401 agtccgccgc ccatgagtac atccggcgcc atttccaagt agcgctgcaa tatcgccgga
     8461 aaatctttcc ggttaggcac atggcagaga aacgccgccg ggctggcgtc gtagatcgtt
     8521 gaattggtgg cgagcccgag tttcatgccg cgccgacgcg caatctccat cgcgctcacg
     8581 gtgatgctgt catcggcgca gactccgaga gcgccgattt tggctttgca accgatggcc
     8641 atcgctgtgg ccgcagccgc ggaatccgtc gacagtgaat tggcggcgtg ggtggtcatc
     8701 acgccgacgc tgccttcctt catgatttta tcgacgatca ccatgccttc gttgtggatg
     8761 tgctggcgat actgg
//
LOCUS       DASQKS010000521         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3664732, whole genome shotgun sequence.
ACCESSION   DASQKS010000521 DASQKS010000000
VERSION     DASQKS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3664732"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VE170_13410"
     CDS             <1..1381
                     /locus_tag="VE170_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ16486.1"
                     /translation="STANSTLNLLGTFFKTSWDASGEIPARQVRSRELGRFGSNDPSE
                     GGKSDRQNLSFIYNYTDADQAFTAQTWASWYKLQLWSNFSLFFNDPVNGDAIEQNDKR
                     FLAGNNVNYRRNYHHWGLPMETFVGFQSRFDHIRVGLFHQSDRQRFATESNNLVRQTN
                     LSWFAQQEIKPVEWLRTQLGVRVDTLWYHVGQIGDPASMSSPISGDGSASIANPKLNF
                     IFTPFTDNNVAKSTNLFVNFGGGFHSNDARVFVQDPDKKIPRYWSGELGARSRLFDRL
                     DATFSYWRSYLTSELVFVGDEGTFEPSGASRRQGIESEFRYDILPWLSYDLDLSYTWA
                     KFTNGDKVSLAPRLLVYSGITARHDSGLQARLQMRHIGRRYSIEDGSIKTPTSTIFDL
                     SLKYLWRHYEFFVQLQNLANTKWRGAEHAFESRTAGQVAGGQPGQLDSHFTPGDPFTA
                     KAGITIHLW"
     gene            1483..2733
                     /locus_tag="VE170_13415"
     CDS             1483..2733
                     /locus_tag="VE170_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16487.1"
                     /translation="MENPPEQTTTDPAGKTAIFSIVCGAHFLNHFQSSMLGVLYPVMM
                     RDLGIGFVAIGAITAVYGFVGNFLQAFYGFIVPYVRRGVILGIGNILLGISVVATGFA
                     SSYPFIMFTRVLGGVGTSPQHPVGSTALASYFGKARGRALAFHSTAANLGSLVAPIVA
                     AVLVAYIGWRAIFWIVGIPSILMGVACLAMRDTIKLNPAQIQKSKLTPMGWDAYKRCL
                     KNKNILLISLVLMVGGAGLGHGINETYIVPHFINDLKVDMAYAAFLFTLIQVGGLLGP
                     MAWGWISDRFHRTRTLQVSLLLSALSTVWLSLQDHVSIGLFANLVLYGAVVTSRQTLT
                     QALLTDVADEDILDAAFSLYYCIGFIMSPFWTLLAGWLMSTSGFQTTFGVISCSYFLG
                     MTLLLFFRDPVKRLRPLKAEPAHS"
     gene            2748..2966
                     /locus_tag="VE170_13420"
     CDS             2748..2966
                     /locus_tag="VE170_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ16488.1"
                     /translation="MIDFIDIIGMIGAFGIMSQAGIDQAHAVTGLAEAADRHASDLLI
                     AIMTLVHYNYFANRAEMKWKKSKTTPRP"
BASE COUNT          626 a          890 c          806 g          732 t
ORIGIN      
        1 ttcgaccgcg aattcgactc tgaatcttct ggggaccttc ttcaaaactt catgggatgc
       61 atccggagag attccggcaa ggcaggtgcg cagtcgcgag ctcggtcgtt tcggttccaa
      121 cgatccgagc gagggcggaa agtctgatcg gcagaatctg agttttatat ataactatac
      181 cgatgccgat caagctttta ccgctcaaac ctgggcctct tggtacaagt tgcaactctg
      241 gtcgaatttc agcttgtttt tcaatgaccc cgtcaacggc gatgccatcg agcaaaacga
      301 taaacgcttc ttggccggca acaacgtcaa ctaccgaaga aactaccacc actggggcct
      361 gccgatggaa actttcgtcg gtttccagag ccgcttcgat cacattcgcg tcggcttgtt
      421 tcaccagagc gatcggcaaa ggtttgcgac cgagagcaac aacttggtac gccaaacgaa
      481 cttgagctgg ttcgctcaac aagaaatcaa accggttgag tggctgcgca cccaacttgg
      541 cgttcgagtc gacacgcttt ggtaccacgt cggccagatc ggcgacccgg cctcgatgtc
      601 aagcccgatc tccggtgacg gctcggcgtc tattgcaaat cccaagttga attttatctt
      661 cacgcctttc accgacaaca acgttgccaa gagcactaac ttgtttgtca acttcggcgg
      721 cggctttcat agcaacgacg ctcgtgtttt cgttcaggat ccggacaaaa agattccccg
      781 ttactggagc ggtgagttgg gagcccgttc gcgcttgttc gaccgcttgg acgcgacctt
      841 ctcgtactgg cgctcctact tgaccagcga attggtcttt gtcggcgacg agggcacctt
      901 cgagcccagc ggcgcgagcc ggcgccaggg tatcgagagc gagtttcgct acgatattct
      961 gccctggtta tcctacgatc tggatctctc gtacacctgg gctaagttta ccaatggcga
     1021 caaagtatcg cttgcgccgc gtttgctcgt ctacagcggt atcacggcgc gccacgattc
     1081 cggtcttcag gcaagattgc aaatgcgcca catcggccgc cgctatagca tcgaagacgg
     1141 cagcatcaag acgccgacct cgacgatctt cgatctctcg ctaaaatatc tgtggcggca
     1201 ctatgagttc ttcgtccagc tgcaaaacct ggccaatacg aaatggcgcg gcgcggaaca
     1261 tgccttcgag tcacggaccg ccggtcaagt cgctggcggg cagcccgggc agttggattc
     1321 tcattttact cctggcgacc catttaccgc gaaagctggc attacgattc acctttggtg
     1381 aacggaggtt ggtcatgaaa tatcgtagag tcgcgagggc agcagcgctt ggcttactgc
     1441 gacactggtt ttctcggtaa attgagataa ggaaaacaga tcatggaaaa tcctcccgag
     1501 caaaccacca ccgaccccgc aggcaagacc gcgatctttt cgatcgtctg cggcgcccac
     1561 tttttgaatc attttcagtc gtctatgctg ggcgtgcttt atccggtgat gatgcgcgat
     1621 ctcggtatcg gctttgtcgc catcggtgcg attaccgcgg tttacggttt tgtcggtaac
     1681 tttctccagg cattctacgg attcatcgtt ccgtacgtgc gccggggcgt aatcttaggt
     1741 atcggcaaca ttctccttgg catctcggtc gtcgccaccg gtttcgcttc cagctacccc
     1801 tttatcatgt tcacgcgcgt gctcggcggc gtcggcacca gcccgcagca tccggtgggc
     1861 tcgacggcgc tggcgtctta tttcggcaaa gcgcgcggcc gcgccttggc gtttcactcc
     1921 acggcggcca acctcggcag tctggtcgcg ccgatcgtcg ccgccgtact ggtggcttat
     1981 ataggttggc gtgcgatctt ctggatcgtc ggtattccca gcatactcat gggcgtcgcc
     2041 tgtctcgcta tgcgcgacac gatcaaactc aacccggcgc aaatccaaaa atcgaagcta
     2101 acacccatgg gttgggacgc ttacaagcgc tgtctaaaga acaaaaacat cctgctgatc
     2161 tcgttggtat tgatggtcgg aggtgcgggt ctcggccatg gcatcaacga aacctacatc
     2221 gtgccccatt tcatcaacga cctgaaagtc gacatggcct atgccgcctt tctgttcacc
     2281 ttgattcagg tcggcggcct gctcggcccc atggcctggg gctggatctc cgaccgcttt
     2341 catcgcacac gcacgctgca agtatcgctg ctgctgtcgg cgttatcgac ggtctggctc
     2401 agcctgcaag atcatgtatc catcggctta ttcgctaacc tggttctcta cggcgcggtg
     2461 gtcacctcgc gccaaacctt aactcaagca ttgctcaccg acgtcgccga tgaggacatc
     2521 ctcgacgccg ctttcagtct ctactattgc atcggtttca tcatgtcgcc gttctggacg
     2581 cttttggccg gctggctgat gagcacttcg ggatttcaaa ccacttttgg cgtgatctct
     2641 tgttcctatt ttctgggcat gacgttgctc ctattcttcc gcgacccagt gaagcggctc
     2701 cgcccgctca aagcggaacc ggcgcattcc taaagcgatt actttaaatg attgacttca
     2761 tcgatatcat cgggatgatc ggcgccttcg gcattatgtc gcaggctggc attgaccaag
     2821 cacacgcggt caccggcctc gccgaagcgg ctgatcgcca cgccagcgat ttgctgatcg
     2881 cgatcatgac cctggtgcac tacaactact ttgccaatcg cgctgagatg aaatggaaga
     2941 aatcgaagac tacgcctcgc ccttgaaccg gcctcggtgg cgacaaagag tacaagtaat
     3001 gcgctcgcgc tggcgagaaa tcgagaacat gggtattgct tcaaggtggt gaaa
//
LOCUS       DASQKS010000522         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5322381, whole genome shotgun sequence.
ACCESSION   DASQKS010000522 DASQKS010000000
VERSION     DASQKS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5322381"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VE170_13425"
     CDS             <1..470
                     /locus_tag="VE170_13425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HYJ16489.1"
                     /translation="GDSASSSELYAILSSLAEMPIQQGIAVTGSVNQNGEVQAIGGVN
                     FKIEGHFDVCRLKGLTGEQGAMIPQANLHNLMLRSDVVDAVKAGKFHIYAVSSIDEGI
                     EVLTGVAAGARQADGSYPDGTINNRVQKKLLRFTELQKKLTAGDQESATAANN"
     gene            complement(668..1729)
                     /locus_tag="VE170_13430"
     CDS             complement(668..1729)
                     /locus_tag="VE170_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ16490.1"
                     /translation="MQYLILSLLLTSLFFPASLRGETKLDKSSLVNWREAQSPTSDSK
                     LDWLSRAFVELAQNSRPAVVQIRVNGQDNSPTQGSRGSGFFIDPEGYILTAQHVIDKA
                     KEIEVRTADGQRLAARLVVADSQLDLALLKIQSDREVPVLTLGNSEGIQVGDLAIVFS
                     YPFGRESSMSLGIISRAGRTYPDSAGYDFVQTDAGAYPGVSGGPLLNSQGHVVGMITM
                     ASERGNLGFATPINLIKKILPRLAKGEKFAWGWLGVQMSDISLGQARSLGIYPAKGVV
                     VSSVLPGQPAARVGVQKQDVIVAVNDTQVDSPRDVTRMVGGLEAGHMVQLSILRKGKP
                     IRVTVPLGIKPESAKPREG"
     gene            1848..2627
                     /locus_tag="VE170_13435"
     CDS             1848..2627
                     /locus_tag="VE170_13435"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16491.1"
                     /translation="MNGGADKIVLVTGGSGGIGAAIAGQFATLGCRVVIAARNSSKLE
                     TAAAKIAENGAQVRAIACDVTRRGEVQRLRLAIQEMWGAVQILVNNAGIASAVSFADM
                     SDETWDRTLDTNLSGAYNCCKVFLPAMIEAQWGRIVNIASTAAKVGYSHVTAYSASKH
                     GLLGLTRSLALETARQGVTVNAICPGYVDDERTRENARIMAEKTDKSGDDILKIFAAS
                     APQNRLIEAAEVATLACLIASPEMSGMTGQAINVDGGAVMA"
     gene            2655..3449
                     /locus_tag="VE170_13440"
     CDS             2655..3449
                     /locus_tag="VE170_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HYJ16492.1"
                     /translation="MVYDSFLFEVADHIATIRLNDPERLNALTFETYAELARVFAAME
                     ADRGIKVVVITGVGNGFCSGGSVHGIIAKLLEMTADEQLRFTTLTCAVVKNMRALNKP
                     IVAAVNGIAAGAGTILALASDIRLLSERAKFAFLFTKVGLSGADMGACYLLPRIVGHG
                     RAAELLFTGDTIDAQECFRIGVANRLIAHEQLLDETYALARRLKEGPLKAIGITKALL
                     DREADMDLANALDLEAREQARLMQTPDFKEGYWAFIEKRPAKFNRS"
     gene            3463..>3974
                     /locus_tag="VE170_13445"
     CDS             3463..>3974
                     /locus_tag="VE170_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16493.1"
                     /translation="MLPFFTHDQAALQAQVRLWTEANLFAGHGSEDLDRCARDAVSQL
                     GAAGFLRHVVSKQFGGTRQKVQARDLCILREELARGDALTDTMFALQALGSYPITLAG
                     SVAHKQSYLPLVASGDAIAAFAITEPEAGSDVASLQTTALRRGEHFVLNGTKRFISNA
                     GIADFYVVFAS"
BASE COUNT          844 a         1116 c         1190 g          824 t
ORIGIN      
        1 acggcgacag cgcgtcctcc agtgaactct acgcgatcct ttcgagtctt gccgaaatgc
       61 cgatccagca aggcatcgcc gtcaccggtt cggtgaatca aaatggcgaa gtgcaggcga
      121 tcggcggcgt caatttcaaa atcgaaggcc acttcgatgt ctgccgcttg aaaggactga
      181 ccggcgaaca gggtgcgatg attccgcagg ccaacctgca taacttaatg ctccgctccg
      241 acgtcgtcga cgcggtcaag gccggcaagt ttcatattta cgctgtaagt agcatcgatg
      301 aaggcatcga agtgcttacc ggagtcgccg ccggtgcgcg ccaagccgac ggcagttatc
      361 ccgacggtac gatcaacaat cgggtgcaga agaaattgct gcgttttacc gagctgcaaa
      421 aaaaattgac cgcgggcgat caagagtcgg cgacggcggc gaacaattga tgtcgcgctc
      481 gaagatggtt gaaatgcctc ggcgaccgcc gccgtgaacc gttgtcatgc gggagcaagt
      541 cggcatccat tccttccgcc gaatccggat accgtttttc aaccttgtga cgcaatcacg
      601 agtggacctc taggcgacaa gctcagaatc cctcggagtg cgagcgagca tcgttcagca
      661 ttctacgcta cccctcgcgc ggcttggctg attccggctt gataccgagg ggtacggtca
      721 cgcgaattgg cttgcctttg cggagaattg aaagttgcac catatggccc gcttccaggc
      781 cgccaaccat gcgagtcaca tcgcgcgggc tatcgacttg agtgtcattg accgcgacga
      841 tgacatcctg cttctgcacg cccacccgtg ctgccggttg cccgggtaaa accgagctca
      901 ctaccacccc tttggccgga tagattccta acgatctcgc ctggcctaaa gaaatgtccg
      961 acatttgcac gccaagccag ccccaggcga acttctcacc tttggccaac cggggcaaaa
     1021 ttttcttgat taaattgatc ggtgtggcga agcctagatt gccgcgctcg gaggccatgg
     1081 tgatcatccc gacgacatga ccctggctgt tcagcagcgg accgccgctg acgccgggat
     1141 aggcgccggc atcggtttga acaaaatcgt aacccgccga gtcgggatag gtacgcccgg
     1201 cgcggctgat gatgcccagg ctcatggaac tctcacgacc aaacgggtag ctgaaaacga
     1261 tcgccaagtc gccgacttga atgccctctg aattgccgag agttagcacc ggcacctctc
     1321 gatcagattg gatcttcaac agcgccaggt cgagttgact gtcggcgacg accaggcgtg
     1381 ccgccaaacg ctggccatca gcggtgcgga cttcgatctc cttggctttg tcgataacat
     1441 gctgcgccgt caggatatag ccttccggat cgatgaagaa gcctgagccg cggctgccct
     1501 gggttgggct gttgtcttga ccgttgacgc gaatctgcac gacggccggg cgggaattct
     1561 gcgctagctc gacaaaggcg cgactgagcc agtcaagctt actgtcgcta gttggcgatt
     1621 gtgcttcgcg ccagttaacc agtgaagatt tgtctagttt ggtttcgccc cgcagcgagg
     1681 ccgggaaaaa tagtgaagta agcaacagcg ataagattag atattgcacg aatccacctc
     1741 ttagcgagtc tggctggcaa gtccctttat cgaactcctg cggcgatcac aagcgccgca
     1801 ctggccggtc ttgcgcttca cgccagggca tatgctatgg ttcgagcatg aacggcggcg
     1861 ccgacaaaat agttttggtc accggcggca gcggcggcat cggcgcggcc atcgccgggc
     1921 aatttgccac gctgggatgc cgggtagtta tcgcggcccg caattcctct aaactagaga
     1981 ctgcggcggc caaaatcgcc gagaatggcg cgcaagtccg tgcgatcgcc tgtgacgtga
     2041 cgcgtcgtgg ggaggtgcag cggctgcggc tagcgattca agaaatgtgg ggggcggtgc
     2101 agatcctagt caacaatgcc ggcatcgcca gcgctgtcag ctttgccgac atgtccgatg
     2161 aaacctggga ccgaaccttg gatacgaatc tgagcggggc ctacaattgc tgtaaagtgt
     2221 ttttaccggc gatgatcgaa gcccaatggg gccgcatcgt caacatcgcc tcgaccgcgg
     2281 ccaaagtcgg ctacagccat gtgaccgcct acagtgcgtc gaagcatggg ttgctcggac
     2341 tgactcggtc gttagcgctg gaaaccgcgc gccagggtgt aaccgtgaat gcgatttgtc
     2401 cgggctatgt ggacgacgaa cgcacccgcg agaacgcgcg cataatggcc gagaagaccg
     2461 acaagagcgg cgacgatatt ttaaaaattt tcgccgccag cgccccgcag aatcgtttga
     2521 tagaagctgc ggaagttgcg acgctggcat gtttgatcgc gtcgccggaa atgagcggta
     2581 tgaccggcca ggcgatcaat gtcgacggcg gcgcggtgat ggcgtgagaa agaattgata
     2641 tttgagaaaa gccaatggtt tacgattcat tcttgtttga agtcgccgac catatcgcga
     2701 cgatacgttt gaatgatcct gagcggttga acgctttgac tttcgagacc tatgcggagt
     2761 tagcgcgggt gttcgcggcg atggaggcgg accgcggcat caaagtcgtc gtgatcacgg
     2821 gtgtcggcaa cggattctgc tccggcggca gtgttcatgg aattatcgcg aagttgctgg
     2881 agatgaccgc cgacgagcaa ctgcgcttta ccacgctgac ctgcgcggtc gtgaagaaca
     2941 tgcgggcgct caataaaccg attgttgccg cggtgaacgg tatcgccgcc ggcgccggca
     3001 cgattctcgc cttggcgagc gatatccgtt tgttgtccga gcgcgccaag ttcgcttttc
     3061 tctttaccaa ggtcggtttg tcgggcgcgg acatgggcgc ttgttatctg ttgccgcgga
     3121 tcgtcggcca tggccgcgcc gccgaattgc tgtttaccgg ggacaccatc gacgcccagg
     3181 aatgttttcg cattggcgtg gcgaaccgtc tgatcgccca tgagcaattg ctggatgaaa
     3241 cctacgcctt ggcgcgacgg ttgaaagaag gcccgctcaa agccatcggc atcacgaaag
     3301 cgctcctcga ccgtgaggcc gatatggatc tggcaaacgc attggatttg gaggcgcgcg
     3361 agcaagcgcg cctgatgcaa acgccagact tcaaagaagg ctattgggct tttatcgaga
     3421 aacggccggc taagttcaat cgttcctaaa tcgattctct ccatgttgcc gttctttact
     3481 cacgatcaag ccgctttgca ggcgcaggtg cggctgtgga cggaagcgaa cctattcgcc
     3541 ggccacggaa gcgaagatct agaccggtgc gccagggacg cggttagtca actgggcgcc
     3601 gctgggtttc tcagacatgt cgtgtcgaag caattcggtg gaacgcgcca aaaagttcaa
     3661 gcgcgtgatc tttgcatctt gcgcgaggag ctggcgcgcg gcgatgccct caccgacacc
     3721 atgttcgcgt tgcaagcgct gggcagctat ccgattacac tggccggcag cgtggcacat
     3781 aaacaaagct accttccgct agtggccagc ggcgacgcga ttgcggcgtt cgcgataact
     3841 gagccggagg cgggttccga cgttgcttcg ttacaaacga ccgcgctgcg ccgtggcgaa
     3901 catttcgttt tgaacggcac caagcgattt atctccaacg ccggcatcgc agacttctac
     3961 gttgtcttcg catc
//
LOCUS       DASQKS010000523         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2405759, whole genome shotgun sequence.
ACCESSION   DASQKS010000523 DASQKS010000000
VERSION     DASQKS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405759"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="pgeF"
                     /locus_tag="VE170_13450"
     CDS             <1..794
                     /gene="pgeF"
                     /locus_tag="VE170_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HYJ16494.1"
                     /translation="KHNPPMELTQLTVPRWQKYDGLLHGFLGRRGGKSVGRYAGLNVS
                     YRVGDDNKVVSQNVCDVKLAMGIHDGKIVTMQQVHGDNLIEVTDKQTKEAGAADSMVT
                     AQNGIFLGVLTADCVPLLFIAPKQRLAAAVHAGWRGTLAGIASKTVDYFMSRYGVAAG
                     DLEVALGPSIGVCCYEVNDDVATPLMKKWGALTTPSIAMKDGKPHINLSRLNRDILRD
                     AGVPGTQLFQIGPCTRCAAEDFFSYRREGGETGRQIGVVGWQAPL"
     gene            complement(846..1127)
                     /locus_tag="VE170_13455"
     CDS             complement(846..1127)
                     /locus_tag="VE170_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16495.1"
                     /translation="MVGYHTAMAIPSTEQQQVGPLWALNFPKRKWRPQSLSACVNLCQ
                     IAQSTAQELFMDSNSEDARLRHALTILGIPESEFYKFQKEQERHANMLR"
     gene            1359..2321
                     /locus_tag="VE170_13460"
     CDS             1359..2321
                     /locus_tag="VE170_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16496.1"
                     /translation="MKISRRHFLTKITVGVAGVSLAREFSWAQVKGKVGYMRIVDNAA
                     MFMAMEKGFFKAEGLELETVPMAGGAVIVQGVTSGDLQFGWTNVISLYQAHAEGFDFK
                     LIAGGATNVRGSNETHAIVVGKDSPIRSAKDLEGKTVAVNTLNNIVHLLALAWVDKNG
                     GNASKVKFVEIPFPQMEPSLIAGKVDAISVQEPFAAVAARRPEARVLANPWGDVLPRF
                     LIASWFASDKWIQKNRGVAQGFVRAINKGVDAIRTDKEGARAAMIKWAGLSPDFASKI
                     GLPEFEKSIAEKDVQVTMDLTYKYKMISKPLKAKDVLSDLATKA"
     gene            2482..3201
                     /locus_tag="VE170_13465"
     CDS             2482..3201
                     /locus_tag="VE170_13465"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16497.1"
                     /translation="MAIGNITCQVEAGEFVSFVGPSGCGKTTLLMSIAGLLAPSSGRV
                     IVKGKEVSGPPVNLVLLFQEYNKSLFAWRSVLGNVGFGLTARGARLTGAAAEDKARKL
                     IELVGLKGFESHYPWELSGGMQQRVAIARALAYEPEVLLMDEPFGSLDALTRLELEDT
                     LLRLWAELKTTILFITHDIEEAIYLSDRIWVLSRRPSKIIEELKVDFPRPRHQVTTRA
                     QARFMELRNAIYRQIGNQGTP"
BASE COUNT          708 a          899 c          989 g          720 t
ORIGIN      
        1 ataaacataa tccgcccatg gaattgactc aattaacggt cccccgatgg cagaagtacg
       61 atggtttgct gcatggcttt ctgggtcggc gcggcggcaa aagcgtcgga cgctatgccg
      121 gtcttaacgt ttcctaccgc gtcggcgacg ataacaaagt ggtcagtcaa aacgtttgcg
      181 atgtgaagct ggcgatgggt atccacgacg gcaagatcgt taccatgcaa caggtgcatg
      241 gcgataatct catcgaggtc acggacaagc agacgaaaga agccggcgca gccgactcca
      301 tggtgaccgc ccagaacggt atctttctcg gtgtcctcac ggcggactgc gtgccgctgt
      361 tgttcattgc acctaaacaa cgtctggccg cagcggtcca cgcgggctgg cgcggcacgc
      421 tcgccgggat tgcttcgaag accgtcgatt attttatgag ccgttatggc gttgcggccg
      481 gcgacctgga ggtggccttg gggccgtcca tcggcgtgtg ctgctacgaa gtcaacgacg
      541 atgtcgcaac accgctgatg aaaaaatggg gagcgctgac gacgccgagc atcgcaatga
      601 aagacggtaa gccgcatatc aacctgagcc ggctcaatcg cgacatcctg cgcgacgccg
      661 gcgtgcccgg cacgcaactt ttccaaatcg gtccttgcac gcgctgtgcc gccgaagatt
      721 tcttttccta tcgccgcgaa gggggggaga ccggccggca gatcggtgtg gtcggttggc
      781 aggcgcccct ataaccgctg gatgcccggc ttggctgctc tcgtcctagg tgacaccgtt
      841 tgcagttaac gaagcatgtt agcatgacgt tcctgctctt tttgaaactt gtaaaactcg
      901 gattcgggaa tccccaagat tgtcagcgcg tgacgtaatc gagcgtcttc actattgctg
      961 tccataaata gttcttgtgc cgtgctttga gcgatttggc agagatttac gcaagccgac
     1021 aaggattgcg gacgccattt ccgcttagga aaatttagcg cccaaagcgg tccgacttgt
     1081 tgctgttcgg ttgacggtat agccatggcg gtatggtagc cgaccacggg ttatttctcc
     1141 acatttgttt cctctctcga aaatgcgtct aaagatttca cttgctggct tgaggcgtct
     1201 aaatcggccc taatcatcca taaaaggcgt cttgggcgat ccgctgatgg ccgacatgcg
     1261 cgagcgtcgg gatcgcctca ctttcattat tcgcttactt atattaagga agaggcgtcg
     1321 gattcgaatc gttgtcacga tatttggggg tcagcgctat gaaaatttcc cgtcggcatt
     1381 ttctcaccaa aatcaccgtc ggtgttgctg gagtttcgct cgctcgggaa ttctcttggg
     1441 cgcaggtcaa gggtaaagtg ggctacatga ggatcgtcga caatgcggcg atgttcatgg
     1501 cgatggagaa gggttttttc aaagcggaag gcctcgaatt agaaaccgtg ccgatggcgg
     1561 gcggcgcggt gattgtccag ggcgtaactt caggcgactt gcaattcggc tggaccaacg
     1621 tgatatcgct ataccaagcc cacgccgaag gtttcgattt taagctcatc gccggcggcg
     1681 cgaccaacgt acgcggcagt aatgaaaccc atgcgatcgt cgtcggtaaa gactcgccga
     1741 tcaggagcgc caaggatctc gaaggcaaga ccgttgcggt gaacacgctc aacaacatcg
     1801 tccatttact cgccctcgcc tgggtcgaca aaaacggcgg caatgcgagc aaagtcaaat
     1861 tcgtcgagat tcccttcccg caaatggagc cgtcgctaat cgccgggaaa gtcgatgcca
     1921 tcagcgtgca ggagcctttc gccgcggtgg cggcacggcg gccggaagct cgtgtgttgg
     1981 cgaatccctg gggcgatgtc ctaccgcgat ttctcatcgc cagctggttc gcgtcggata
     2041 aatggattca gaaaaaccgc ggcgtcgcgc aggggtttgt gcgcgcgatc aataaggggg
     2101 tcgacgccat ccgcaccgac aaagaaggcg cgcgcgcggc gatgatcaaa tgggccgggc
     2161 ttagccccga cttcgccagc aagatcggct tgccggagtt tgagaaatcg attgctgaaa
     2221 aagatgtcca agtgaccatg gatctgactt acaaatacaa aatgatatcc aagccgctca
     2281 aagcgaagga tgtgctgagc gatctggcga caaaagcgta aacttcaatt ggagtcctgg
     2341 aggattggag caatcggttc ggaaagcccc aatactccag tgctccatca atccagcact
     2401 ccattccgcc actctcccgt gccgattctc gaggttcaat cactttcgaa aagttaccgg
     2461 caagccggtg gcgagactac cgtggcgatc ggaaatatta cttgccaggt cgaggcgggg
     2521 gagtttgttt ccttcgtcgg gccgtccggg tgcggcaaga cgacgctgtt gatgtcgatc
     2581 gccgggctgt tggcgccgag ttccggccgc gtcatcgtca aaggcaagga agtcagcggg
     2641 ccgccggtca acttggtgct gttgtttcag gagtacaaca agtcgctgtt cgcctggcgg
     2701 tcggtgctcg gcaatgtagg tttcggcttg acggcgcgcg gcgcgcggtt gaccggggcg
     2761 gcagcggaag ataaggcgcg taaactaatc gagctggtcg gtttaaaagg atttgaaagc
     2821 cactatcctt gggaactctc cggcggcatg cagcagcggg tcgcgatcgc ccgggcgctc
     2881 gcctacgagc cggaagtttt gttgatggat gaaccgttcg gttcgctcga cgcactcacc
     2941 cggctggagc tcgaggacac gctgctgcgg ctgtgggcgg aactcaagac cacgatcctg
     3001 ttcatcaccc acgatatcga agaagcgatt tatctttccg accgcatctg ggtgctgagc
     3061 cggcggccgt cgaaaatcat tgaagaactc aaagtcgatt tcccccggcc gcgccatcaa
     3121 gtaacgacac gcgcgcaagc ccggttcatg gagctgcgca acgctattta ccggcaaatc
     3181 ggcaaccagg gcacgccgtg aagcacgcgg caccttttaa ctggctgggt tggtcgagct
     3241 ttgctttatt gctggccttg tgggagctgg gcgcgcgcgc gacccccaag ctcgtactct
     3301 atttcccgcc ggtgag
//
LOCUS       DASQKS010000524         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5742677, whole genome shotgun sequence.
ACCESSION   DASQKS010000524 DASQKS010000000
VERSION     DASQKS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5742677"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..794
                     /locus_tag="VE170_13470"
     CDS             357..794
                     /locus_tag="VE170_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYJ16498.1"
                     /translation="MSVNKVILVGNLGKDPDVRYTQTGSAVANFSIATSEQWNDRDGK
                     RQERTEWHNIVVWGKQAEHCGQYLSKGRQVFIEGSIRTRSYDDKSGNKRYVTEIIAQR
                     IQFLGGGGGSRGPQPGMDTGGVDDIAMGGMGGGQPPSDDDIPF"
     gene            896..>2539
                     /locus_tag="VE170_13475"
     CDS             896..>2539
                     /locus_tag="VE170_13475"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYJ16499.1"
                     /translation="MERSPLQEIDVDAALRTILEGTATETGAGFFRALVKNLAQALGT
                     HGAWVTEYFPESRRLRDLSFWLGDRWLDGWEMVVDGTPCERVIDDRCLIHIPDNLLDI
                     YGNDPDVRAMGAASYMGMPLLDLDDAILGHLAVLDMRPMPAEPRAQTLFQIFAARAGA
                     ELRRLRAETQVLEREQKLGRLVGSAMDAIIELDSDLTITQMNPAAERVFGCDAGAMIG
                     EAFNHRLVPDAREKLARLIIELDTRPQSQRWLWVGGGFHAIAHGGASFQAEATLARFD
                     VEREPYYTLILRNVNERIEAEHKIHSLTVEAEYLREEIKRLVGADGIVGQSGSWLKIL
                     SEAQEVAATDATVLILGETGTGKEVIARSIHRASRRHNRPLIKVNCAAMPAALIESEL
                     FGHEAGAFTGATKKRDGRFAMAHGGTIFLDEVGELPFDLQSKLLRVLQEGEFEPVGSS
                     QTRRVDVRVLAATNRDLQRFTGEGKFREDLYYRLNVFPIELPPLRERREDIGVLAEAF
                     AEKFAKSMGRPIEPLSVDCIRRLEAYRWPGNVRELQNIIE"
BASE COUNT          549 a          695 c          772 g          523 t
ORIGIN      
        1 ttcgacggtc aaagcgatgc cgctgaattt gacaatcaac caagtccaaa attgattgat
       61 gcgatatacc cgtctgcctt gggcatcgaa caggccgcat agaaccgtgg ccagagaaag
      121 caccatggtg gctgctatga cgattgctaa tttgactgcg taaaacatca agacaacgtt
      181 aaatttgaag cgccgaactg attggaaatt attggctaat tgtagagaag gagtgggcaa
      241 aaatcaatgt gaggaagttc gtgctacttc agcgccatcg agttgactct cccggtgggc
      301 agggataagt tgagtgagct tttccgccgc agactttttc cggtttcgga ggcagcatgt
      361 ccgttaacaa agtcattctg gtcggcaatc tgggcaagga tcccgacgtg cgttataccc
      421 agacgggcag cgccgtggca aatttctcga tcgccacctc cgagcaatgg aacgaccgtg
      481 acggcaaacg ccaggagcgc accgagtggc ataatattgt ggtctggggt aagcaggccg
      541 agcactgcgg acagtatcta tcgaagggcc ggcaggtttt tatcgaaggc agcattcgca
      601 cgcgcagcta cgacgataag agcggcaata agcgctacgt caccgagatc atcgcccaac
      661 ggatccagtt ccttggcggt ggcggcggca gccgtggccc acaaccaggt atggacacag
      721 gcggcgtcga cgatatcgcg atgggcggca tgggcggcgg ccagccgccc agtgacgacg
      781 atattccatt ttaaggtaac cgccgcgccg atcggttcca atcaccgcca tcacgtacag
      841 atgagcggtt taacaacgcg acttaacagt tttcttccgt gcttcatgct aagccatgga
      901 acgctcgccg ttgcaagaaa tcgacgtcga tgccgcgctg cgcacaatct tagaaggaac
      961 cgcgactgaa accggcgctg ggtttttccg agcgttggtg aaaaatctag cccaggcgct
     1021 cggtactcat ggcgcctggg taacggaata ctttcctgaa tctcggcgac tgcgggatct
     1081 gtcgttttgg ctcggcgacc gctggctcga cggctgggaa atggttgttg acggcactcc
     1141 ttgtgagcgc gtgatcgacg accgctgctt gatccacatc cccgacaatc tcctcgatat
     1201 ctatggcaat gacccggacg tacgggcgat gggcgcggct agttatatgg gcatgccgct
     1261 gctcgatctc gacgacgcga ttctcggcca tctcgctgtc ctcgacatgc ggccgatgcc
     1321 cgccgagccg cgggcgcaaa ctttatttca gatctttgct gcgcgcgccg gcgccgagtt
     1381 gcgccggcta cgcgctgaaa cccaagtgct cgaacgcgag caaaagctcg gtcgcttggt
     1441 cggcagcgcg atggacgcga tcatcgaact tgacagcgat ttgacgatca cgcagatgaa
     1501 tcccgccgcg gagcgagtat tcggatgtga cgccggcgcg atgatcggcg aggcttttaa
     1561 tcatcggctc gtgccggatg cgcgcgaaaa gctcgctagg ttgatcatcg agctcgatac
     1621 ccggccgcag agccagcgat ggttatgggt gggcggcggt tttcatgcca tcgctcatgg
     1681 tggcgcttct tttcaagccg aggcgaccct ggcgcgtttc gatgtcgaac gggagcctta
     1741 ctacactctc atcctgcgca atgtgaacga gcgcatcgaa gcggagcaca agattcattc
     1801 cctcaccgtc gaggccgaat atctgcgcga agaaattaag aggctggtag gtgccgacgg
     1861 catcgttggc cagagcgggt cttggctaaa aatcttgagc gaggcgcagg aggtcgcggc
     1921 caccgatgct acggtcttga tcctcggcga aaccggtacg ggaaaggaag tgatcgcgcg
     1981 atctatacat cgcgctagcc gccgccacaa ccggccgctc atcaaggtga attgcgcggc
     2041 gatgccggcg gcattgatcg agagcgaact cttcggccat gaagcgggcg cctttaccgg
     2101 cgccaccaag aagcgcgatg gccgctttgc catggcccat ggcggcacga tatttctcga
     2161 tgaagtgggc gaactgccgt tcgacttgca gagtaaattg ctgcgggtat tgcaagaagg
     2221 cgagttcgag ccggtgggca gttcgcagac ccgaagggtg gacgtgcgtg tcttggccgc
     2281 caccaatcga gaccttcaac ggttcacggg cgagggtaaa ttccgcgagg atctttatta
     2341 tcgcttgaat gtttttccca tcgagttgcc gccgctgcgt gaacggcgcg aggatatcgg
     2401 cgttctggcc gaggccttcg ctgaaaagtt cgccaagagc atgggccggc cgatcgaacc
     2461 gctgtcggtg gactgtatcc gccgcctgga agcttatcgt tggcccggca acgtccgcga
     2521 actgcaaaat attatcgag
//
LOCUS       DASQKS010000525         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4892470, whole genome shotgun sequence.
ACCESSION   DASQKS010000525 DASQKS010000000
VERSION     DASQKS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4892470"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VE170_13480"
     CDS             <1..474
                     /locus_tag="VE170_13480"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase"
                     /protein_id="HYJ16500.1"
                     /translation="SDIEMGKRLSDKYGVPVSPGITGEIEALKSLSIHKLVVATPHED
                     TLNERMKAFLQASGFKVLKIQGYGVRKNADITDMDEHAAYKIAKRLYESAPEADGVFV
                     PCPRWPTITDVDLLEREIGKPVITSSQAYIWHALKMARVKQEVTGYGRLMASLAN"
     gene            823..1785
                     /locus_tag="VE170_13485"
     CDS             823..1785
                     /locus_tag="VE170_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16501.1"
                     /translation="MPKSSRQKLFTVVLVLNFVISAGCAQLSRERDSRAEIGFIDINS
                     DIKLRRMIVRNARPKGVVLFLHGFPETLYAWKRISVILARDYEVHAFDWPGYGLSSRP
                     PADQFSYAPRDYAQVLKEYIRKSGIDASKLVIYATDIGALPALIVALDEPNFAQQIIV
                     GDFAPFDRPKYMHANLQSLKSKPSSDKTRAHMNNTRDEILENAFRRGLSTDEQFELSQ
                     EFRDDIFRGWSQDNMTSADAFYHYYSHFTRDQNYLESNLNRLKTPVTVVWGEKDFYIK
                     KEMGAEFANKAKLKLFVLSGLGHYPHLQSPKRTIDEIRSSFDRR"
     gene            complement(2060..2800)
                     /locus_tag="VE170_13490"
     CDS             complement(2060..2800)
                     /locus_tag="VE170_13490"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HYJ16502.1"
                     /translation="MIKIWLTVGFFLLAVSPAAAQFDKLLKDFGGKLPSGGGLSAGGL
                     SDAKVGSGLQEALKVGTENAVQQTGSVDGFLMNKAIKILMPKPLQTMEQPLRLVGYGP
                     QIDEFIVGMNRAAEKAVPFAKDIFWSAIGEMSFDDARKILEGSDTAATDYFKGKTSKK
                     LQTAFKPSVTDVMNQVGVNQQYNALVSKYKDVPFSKNIAFDVNQYVTEKSTDGIFFVV
                     AQEEKKIRTNPAARVNDLLKEVFGSRKR"
     gene            complement(2848..3249)
                     /locus_tag="VE170_13495"
     CDS             complement(2848..3249)
                     /locus_tag="VE170_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ16503.1"
                     /translation="MSNRIAVLGIALSLVFLTSCSSIDRWGSGRDVFETGLSLFQQGR
                     FAEAIPYLEDATHINPKDGQAFFYLGRAYVSQSKWRKAIAPLRTAFRLSPRDAQEEII
                     NLIIDAGFAAALNDFKLGDIVDGPRRSRELL"
     gene            complement(3328..3651)
                     /gene="grxD"
                     /locus_tag="VE170_13500"
     CDS             complement(3328..3651)
                     /gene="grxD"
                     /locus_tag="VE170_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HYJ16504.1"
                     /translation="MAEDAISKIDEKVKNNKVMVYMKGTPDFPQCGFSAHTVEILKSY
                     GLPFASDDVLADPAVRDGIKRYSNWPTIPQVYIDGKFVGGCDILHELHERGELEPLLK
                     AAFEK"
     gene            complement(3678..3932)
                     /locus_tag="VE170_13505"
     CDS             complement(3678..3932)
                     /locus_tag="VE170_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HYJ16505.1"
                     /translation="MISPDEIKQTLSNALPVSLVETQDLTGGGDHWQVIIVSEAFDGK
                     GLLEQHRMVNEVLKEPLADQRIHALSLKTYSPAQWEKLGT"
     gene            complement(4090..4434)
                     /locus_tag="VE170_13510"
     CDS             complement(4090..4434)
                     /locus_tag="VE170_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16506.1"
                     /translation="MELQKELAVNYCHLAVCCVDSPEINDAFRTGLGAQYPFLSDHER
                     KVVNLLDMTETSKSRGVTAMPHTFSLLPDLTIHNLYNGYWFVGRPTLDELRDDLRAMM
                     RKVRPDYEGPVG"
     gene            complement(4465..4593)
                     /locus_tag="VE170_13515"
     CDS             complement(4465..4593)
                     /locus_tag="VE170_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYJ16507.1"
                     /translation="MNLKIGQKFPDIELPDHDGQSVKLSELVGKFPFILAFYRGYW"
     gene            complement(4643..>5366)
                     /locus_tag="VE170_13520"
     CDS             complement(4643..>5366)
                     /locus_tag="VE170_13520"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16508.1"
                     /translation="AGLQGHDIVLIASSESAYNYSVVAKPAITRVEQLKGKRLGVSGF
                     GSASHNAALILLKKFNLEAGKDVALVVAGPTMERLAAVDAGRIEATILTPSETPRARK
                     QGLIEVYDMLDLGIEVQGNGFATSRGFIRSQRETALSALRAYVEAIYFIGKNKDESKR
                     IAAKYMRTNDQEVLDATYDWFTKRVAKRPYPTLKGIQYLLDELAPKVAQAKTAKPEQF
                     VDLSLLQQLEKDGFFTEMTKRY"
BASE COUNT         1222 a         1530 c         1397 g         1217 t
ORIGIN      
        1 agcgatatcg agatgggcaa gcgcttgagc gacaaatacg gtgtgccggt ttcccccggc
       61 atcaccggtg aaatcgaagc gctcaaatcc ctcagcatcc acaagctcgt cgtcgccacg
      121 cctcacgaag acacgctcaa cgagcgcatg aaggcatttc tccaagcgtc agggtttaag
      181 gtcctaaaaa tccaaggcta cggcgtgcgc aaaaacgccg acatcaccga catggacgag
      241 cacgccgcct acaagatcgc caagcggctc tacgagtcgg cgcccgaggc cgacggcgtc
      301 ttcgtcccct gcccgcgctg gccgaccatc accgatgtcg atctgctgga gcgcgagatc
      361 ggcaaaccgg tgatcaccag ttcccaggct tatatctggc acgcgttaaa gatggccagg
      421 gtaaagcaag aagtgaccgg ttacggcaga ttgatggcgt cgttggcgaa ctgattaccc
      481 aatgaatcta agattgtagg tcgggttagc tacgcctaac ccgacctacg cgccttagtg
      541 gtcaggcacg ggtgcctttg tgaataactt cagcgagttt tccaattccc ctcggaaaca
      601 tttcgccacg cggagtctct atccggccac ttagtgacgg caactccacc gtgcgggtca
      661 aagtcgaaac gcccgttgca cacgttggtc ggcattgtat gacgtgaacc ggttttgaaa
      721 gccaacggca cacgcgggtc agagtttttc agaaaccgca tgtcgactga gccgtcgcag
      781 agataaaggc gaacctgcgc cgcaccccaa aggatcgctc aaatgccgaa atcatcgaga
      841 caaaaattgt ttacagttgt actcgtactc aatttcgtga tttcagctgg gtgtgcccaa
      901 ctctcgcgtg agcgagattc gcgagcggaa atcggcttca tagacatcaa ttccgacatc
      961 aagctcagaa gaatgattgt tcgcaacgcg agaccgaaag gcgttgtgct cttcctgcat
     1021 ggctttcctg agactctgta tgcctggaag cgcatctccg tgattctcgc cagagactac
     1081 gaagtacatg cttttgattg gccgggctac ggtctttcct cgagaccgcc tgctgatcaa
     1141 ttttcgtacg cgcccagaga ctatgcgcag gttctgaagg aatacatcag gaaatcaggt
     1201 attgatgctt caaagctcgt gatctatgcc acggatatcg gcgccttgcc cgcccttatt
     1261 gttgctttag acgaacccaa tttcgcgcaa caaataattg ttggtgattt cgccccattt
     1321 gataggccta agtacatgca tgccaatttg caaagcctga aaagcaagcc ttcttctgac
     1381 aagactcgcg cccacatgaa caacactcgc gatgaaattc tggaaaatgc cttcagaagg
     1441 gggttgtcca cggatgagca gttcgaactt tctcaggaat tccgggatga tatttttaga
     1501 ggatggagcc aagacaacat gacttccgcg gacgccttct atcattacta ttcacacttc
     1561 accagggacc agaactattt ggagtccaat ctgaacaggc tcaaaacgcc cgttaccgtc
     1621 gtgtggggtg agaaagattt ttatatcaag aaagaaatgg gagctgagtt cgctaataaa
     1681 gccaaattaa agctctttgt tctttccggt cttgggcact atccgcatct ccaaagccca
     1741 aaacgaacaa tcgacgaaat tcgttcatca tttgatagac gatgaatttc gagagtttgt
     1801 tagatgacat gcgcatatag atctgcgctg gtgtgtcgcc ccctcgtggc tgggctacgc
     1861 accggcgaac cccgctatcc catcgccgtg atcggttgcg gccataggcg ccgcgcaact
     1921 tcaacttgca ctgcgagaat ggtggggact ctcctagttt cagaaggggt aggaagagca
     1981 aaaacaaaac ggcgaacaga ataagctcga aaccatcctg ctcgccgtca accatgctta
     2041 gtctatgaat tcgtagcagc tatcgcttcc ggctaccgaa cacttccttc aacagatcgt
     2101 tcacccgcgc cgccggattg gttctgattt tcttttcttc ctgggcgacg acgaagaaga
     2161 tgccgtcggt gctcttctcc gtgacatatt ggttcacgtc gaatgcgatg ttcttggaga
     2221 acggcacgtc tttatacttg ctgaccagcg cgttgtactg ctggttgaca ccgacctgat
     2281 tcatcacgtc ggtgaccgaa ggtttgaacg ccgtttgcag ctttttcgaa gtcttcccct
     2341 tgaagtaatc cgtcgccgcg gtgtcgctgc cctccaagat cttgcgcgcg tcgtcgaagc
     2401 tcatctcgcc gatcgcgctc caaaaaatat ctttcgcgaa gggcacggcc ttttccgcgg
     2461 ctcggttcat gccgacgatg aactcgtcga tctgcgggcc atagccgacc aggcgcagcg
     2521 gttgttccat ggtctggagc ggtttgggca tgagaatctt gatcgccttg ttcattagaa
     2581 aaccatcgac ggaaccggtc tgttgcactg cgttctcggt gccgaccttg agcgcctctt
     2641 gcaggccgga gccgactttg gcgtcgctca aaccgcccgc gctcaagccc ccaccactgg
     2701 gcagcttacc gccaaaatcc ttcaacagtt tatcgaactg cgccgccgcc ggactcaccg
     2761 ccaacaggaa aaacccaacc gtcaaccaga ttttgatcat ctatttctcc cgtgaaactc
     2821 gcgcggcatt attaattcga atgccggcta taaaagctcc cgcgagcggc gcgggccgtc
     2881 gacaatatcg ccgagcttaa aatcgttgag cgcggcggca aagccggcgt cgattatcaa
     2941 gttgatgatc tcctcctgcg cgtcccgcgg cgacaagcga aaggcggtgc gcaacggcgc
     3001 aattgccttc cgccacttgc tttggcttac ataggcgcgg cccaggtaga aaaaagcctg
     3061 cccgtccttg ggattgatat gggtggcatc ttcgagatag ggaatcgcct cggcgaaacg
     3121 gccttgctga aaaaggctca agccggtttc gaaaacgtcc cgccccgaac cccagcggtc
     3181 gatggaggaa caagaggtga gaaataccaa gcttaatgca atacccaaaa cggcgatacg
     3241 attgctcatt ggactcaacc tcctaaatgg cttagacgca aaaaagcccc gaatcttagc
     3301 gggcagctaa gtccggggct tcctttccta cttctcgaaa gcggctttga gcagcggctc
     3361 gagctcgccg cgttcgtgga gctcgtgcag gatatcgcag ccgccgacaa acttgccgtc
     3421 gatatagacc tgcggaatgg tcggccagtt cgagtagcgc ttgatgccat cacgcaccgc
     3481 cgggtcggcg agcacgtcat cgctggcgaa tggcaggccg taggacttaa gaatctccac
     3541 cgtgtgggcc gaaaagccgc actgcgggaa atccggcgtc cccttcatgt aaaccatgac
     3601 tttattgttc ttaaccttct cgtcaatctt gctgatcgca tcttctgcca taaaaactct
     3661 ccttataacc tttggaatta tgttcccagc ttttcccact gggccggact ataggtcttg
     3721 agggacagcg cgtgaatgcg ctgatcggcc aatggctctt taagcacctc attgaccatg
     3781 cggtgttgtt cgagcaatcc tttgccgtca aaggcctccg acacgataat gacctgccaa
     3841 tgatcgccgc cgccggtcaa atcttgggtt tcgaccagcg acaccggcag cgcattggat
     3901 aatgtctgtt taatctcatc tgggctaatc atggaaaaat cttaccggca accaggcccg
     3961 cttgcaactg tcgggtagcg gggttcagtt ttagagaacc tgcgaagcga ctttgcgcgg
     4021 cgtccgagac gccggacacc tggggtctgc cgccgattga acatcccggc gcagccccca
     4081 tgacaatccc tatcccacgg gcccttcgta gtcgggccgc accttgcgca tcatggcgcg
     4141 caaatcatcg cgcagttcgt cgagggtcgg ccggccgaca aaccagtagc cgttgtataa
     4201 attgtgtatc gtcaggtccg gcaacagtga gaacgtatgc ggcatggccg tcacccccct
     4261 gctcttggaa gtctcggtca tgtcgagcaa gttaacgact ttgcgctcat ggtcgctcag
     4321 aaatggatac tgcgcgccca ggccggtgcg aaaggcatcg ttgatttccg gcgaatcgac
     4381 acagcagact gccagatggc aatagtttac cgccagctcc ttttgcaatt ccacgtattg
     4441 gcgcaactgc acctggcatt tgcctcacca gtaaccgcgg taaaaagcca ggatgaaggg
     4501 aaacttgccg accagctcgg acaatttcac ggactgtccg tcgtggtcgg gtaactcgat
     4561 gtcgggaaac ttctgaccga tttttagatt catagcttct cccttggctt aatcatggac
     4621 tgtaatttgg cttcgaaagt tattaataac gtttagtcat ctcggtaaag aagccgtctt
     4681 tctccaactg ctgcaataag ctcaagtcga cgaattgttc gggcttggcg gtcttggcct
     4741 gcgccacctt cggcgccaac tcgtcgagca gatactgaat ccctttcaac gtcggataag
     4801 gccgctttgc cactcgcttg gtgaaccagt cataggtggc gtccaacact tcttgatcgt
     4861 tggtgcgcat gtacttggcg gcgatacgct tggactcgtc cttatttttg ccgataaaat
     4921 aaatcgcctc gacataggcg cgcagcgcgc tcagcgcggt ctcgcgctgg ctgcggatga
     4981 agccgcgcga agtcgcaaag ccgttaccct ggacttcaat gcccaggtcg agcatgtcgt
     5041 aaacttcgat caaaccctgc tttcttgccc gcggcgtctc ggagggcgtg agaatcgtcg
     5101 cttcgatgcg cccagcgtca accgcggcga gccgctccat ggtcgggccg gcaacgacca
     5161 gcgcaacatc cttgcccgct tccagattga attttttcag caggatcagc gccgcgttat
     5221 gagatgccga gccaaagcca ctgaccccca agcgcttgcc cttcagctgc tcgacccggg
     5281 tgatcgccgg cttggcgacg acagaataat tgtaagccga ttcggacgaa gcgatcaaaa
     5341 cgatgtcgtg gccctgcagg ccggcc
//
LOCUS       DASQKS010000526         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5743403, whole genome shotgun sequence.
ACCESSION   DASQKS010000526 DASQKS010000000
VERSION     DASQKS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5743403"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VE170_13525"
     CDS             complement(<1..715)
                     /locus_tag="VE170_13525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16509.1"
                     /translation="MSNRNKITFGIRLPNSGPLASPQSMLQVAQEAEALGFDSIWVHD
                     HLTWSDEIHRTHISSGSDDSHTGNANPDFFEAMTSLAFIAGQVHKVRLGIACVVIPCR
                     NPLLAAKQIATLDVLCGGRLDIGVGIGSPSTIKSREYEVLGVNRKQRGKIADDHLRAM
                     KTIWTDHPASYDGRFVKFENAEICPKPLQKPNPPLWVGGWTEAAMKRTAALGDGWLPA
                     WLLPQDIGKRFGEVKVMAQS"
     gene            966..2177
                     /locus_tag="VE170_13530"
     CDS             966..2177
                     /locus_tag="VE170_13530"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16510.1"
                     /translation="MKLSRTSILLLCTAAGTITSISMNTFSLFLPPIEHEFAVSRSLA
                     TVPYMVAMLGWGAGALLFGKLADDLGPRPVIIGGILLMASGYVGMSWSRNLWQLSLTY
                     GLMVGLAMGACSQAILSLIVSKHFAATNRGLAVSVVQIASPLSPLLFAPVIYFLIRAQ
                     DWRVAALASGAILFFVALPLAWFGARDPAGSQAGRGPRVPWSACVPYLRSRSMLVLFA
                     VRFSCGVAFFQIAHLVALTISKGFDAATGAKAVSVFGAGAVVSALLFGWLSDRYGRAP
                     MLGLSYLTRGVGTLLLALDISNVYVYYAVVALAIGPTFGTVAVGNVLFYELVGPRMAG
                     MILGMSFIVHQIGSAAGPMIASITYDRTGSYDGFLVVMSLVLLASGLALFLEKNTDAV
                     PVGAASAASPS"
     gene            complement(2195..>2877)
                     /locus_tag="VE170_13535"
     CDS             complement(2195..>2877)
                     /locus_tag="VE170_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16511.1"
                     /translation="DLAKMKTELDAVAQTVNQNGGKAVAITADCSDDRQVNNLVDETV
                     HRFGKIDVLINSAGFRGPLVAVTEITDKEFDDVILYNLKLVFLCCRAVLKQMVKQKSG
                     SIVSISGTAGREGMAMRGSLCAAKWGVVGLTQTIAKEYGPHGIRANIICPGGMDEPDL
                     REMYAQRAKNLGMTVEAHEAQILALTPLRKYAKHDEVANAALFLASSDSSHTTGESLN
                     VSGGRLMS"
BASE COUNT          491 a          803 c          896 g          687 t
ORIGIN      
        1 ggctttgcgc cataactttt acttcgccga agcgtttgcc gatgtcttgc ggcagcagcc
       61 atgccggcag ccaaccgtcg cccagtgctg cggttctctt catcgcggct tcggtccagc
      121 cgccgaccca cagtggcggg ttgggtttct gtaacggttt gggacagatc tcggcgttct
      181 cgaattttac gaaccggccg tcgtagcttg ccggatgatc ggtccaaatg gttttcatcg
      241 cgcgcaggtg atcgtcggcg attttgccgc gctgttttcg attcacgccc agcacttcgt
      301 attcacgcga cttgatcgtc gacggcgagc cgatgccgac gccgatgtcg aggcggccgc
      361 cgcacaggac gtcgagggta gcgatttgtt tggcggccaa aagcggattg cgacagggaa
      421 taaccacgca ggcgattccc aggcgaactt tatgaacctg cccggcgatg aatgccagcg
      481 aggtcatggc ttcaaagaaa tccgggttcg cattgccggt gtgtgaatcg tcggaacccg
      541 atgaaatatg cgtgcgatga atctcgtcgg accaggtcag gtgatcgtgc acccagatcg
      601 aatcgaagcc cagcgcttcg gcttcttgag cgacctggag catcgactgc ggcgatgcca
      661 gggggccgga gttgggaagt cggatgccga aagtgatttt gtttcggttg ctcatgaagg
      721 atgctccttt gggatcaaga agtattacga agatagtgac tcatgcaacc aagcgaaacg
      781 atctcaatcg gcgcagcgac gatcggtaaa taattgcacg gtagttggag gcacaacgcg
      841 acgatgccca tggttagaat acgatgggta tcgctgcgct ccacccatcc tacgtcgact
      901 tgctagatgg caattgagtt gcgaacgcgt tgccagcgcg ccgcggcact ggcatagtcg
      961 gggctatgaa actttcgcgc acttcgattt tgctgctctg cacggccgct ggaacgataa
     1021 cgtccattag catgaacaca ttcagtctgt tcctaccgcc gattgagcac gaatttgccg
     1081 tatcgcgctc gttggcgacg gtgccttata tggtggcgat gttgggatgg ggcgcgggag
     1141 cgctgctgtt cggcaagttg gccgacgatc tggggccgcg gcccgtgatc attggtggta
     1201 tcctgttaat ggccagcggc tacgtcggca tgagttggtc gcgaaacctt tggcagttgt
     1261 cgttgaccta cggcttgatg gtgggtctag cgatgggcgc gtgcagtcaa gcaatcttgt
     1321 cgttgatcgt ttccaaacat tttgccgcga ctaatcgcgg cctcgcggtc agtgtggttc
     1381 agatcgcatc gccgttgagt cctttgctgt tcgcgccggt gatttatttt ttgattcgcg
     1441 cccaggattg gcgcgtggcg gcgctcgctt ccggcgcaat attattcttc gtcgcgctcc
     1501 cgctagcctg gttcggcgcg cgcgaccccg ctgggtcaca ggcaggccgt ggcccgcgtg
     1561 ttccgtggag cgcgtgcgtg ccttatctgc gcagtcgctc gatgctggtg ctctttgccg
     1621 tgagattctc ctgtggcgtg gcattctttc agatcgccca tctcgtcgcc ttgaccatca
     1681 gcaagggctt tgacgccgcc accggcgcca aggcggtgag cgtgttcggc gccggcgcgg
     1741 tggtgtcggc gttgctcttc ggctggctct cggatcgcta cgggagagcg ccgatgttgg
     1801 gcctgagcta tctcacccgc ggcgtcggca ccttgctgct ggccctcgat atcagcaatg
     1861 tctatgttta ctacgccgta gtcgcgctgg ccatcgggcc gactttcggg acggtggcgg
     1921 tgggtaatgt tttgttctac gaactagtcg gcccgcgcat ggccgggatg atcctcggca
     1981 tgagcttcat cgtgcaccag atcggctcgg ccgcgggacc gatgatcgcg agcatcactt
     2041 acgaccgcac cggcagctac gacggtttcc tggtggtgat gagcctggtg ctgctggctt
     2101 ccgggctggc gttgtttctt gagaaaaata ccgacgccgt gccggtgggc gccgcctccg
     2161 cggctagtcc gtcgtagact aaaactgccg acgctcagct catcaatcgc ccgcccgaca
     2221 cattcagtga ctctccggtc gtgtgcgacg aatcgctcga ggccaagaac agtgcggcgt
     2281 tggcgacttc gtcatgtttg gcgtatttgc gcagcggtgt gagggcgagg atttgcgcct
     2341 catgggcttc aaccgtcatg cctaagttct tggcgcgctg agcgtacatt tcgcgtagat
     2401 cgggctcgtc cataccgccg gggcaaatga tgttggcgcg gatgccgtga gggccgtact
     2461 ctttggcaat ggtctgggtc agaccgacca cgccccattt ggcggcgcac agcgagccgc
     2521 gcatcgccat gccttcgcgg ccggcggtgc cggagatgct gacgatgctg ccgctcttct
     2581 gtttgaccat ttgtttcaaa accgcgcggc agcagagaaa taccagcttc aaattgtaaa
     2641 gaatcacgtc gtcgaattct ttgtcggtga tttcggttac cgccaccaat ggaccgcgaa
     2701 aaccggcgct gttgatcagt acatcgatct tgccgaaacg gtgcacggtt tcgtcgacca
     2761 ggttgtttac ttgccggtcg tcgctgcagt cggcagtgat ggcgacggct ttgccgccgt
     2821 tttgattgac cgtttgggct accgcatcga gttcggtttt catctttgcc aaatcga
//
LOCUS       DASQKS010000527         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4471162, whole genome shotgun sequence.
ACCESSION   DASQKS010000527 DASQKS010000000
VERSION     DASQKS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4471162"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VE170_13540"
     CDS             complement(<1..1123)
                     /locus_tag="VE170_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ16512.1"
                     /translation="MANSSIETDVAIIGGGGAGIPAAIEVARAGGKALVLEGAAECGG
                     TAAISGGGCCIVGTPLQKAEGINDTPDLAFEDWVKWGGGQADEIWARYYLEHSLHELY
                     HWAESCGVKWVDMKFQEGNRVLRWHRPDNNGLGLMTALIATAASIPNITIMTATKAGK
                     LITKDGRVAGLTATKGGAAIDILSKTVVVATGGFNSNLEMVLEHRPEFKNFRVMEGSG
                     FGAKGEGHKMIGELGGYFTHMDCIWFYVYATPDYRDPQQRRGLVFRMVPGYIWFNQQG
                     RRFHNESRTGGASATPALLAQTPPHAWAIADTPMLAKIDVADPYYRRGDVIDRAKVEE
                     LLDNSPFIKKADSLEELGKQCRFEDLKNFLGEVEAYNQCF"
     gene            1258..2241
                     /locus_tag="VE170_13545"
     CDS             1258..2241
                     /locus_tag="VE170_13545"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16513.1"
                     /translation="MICRRISLFLIVSFVLMTFANRNGHSQENVRIAHPSLSASVLCL
                     IIANKEGFFREEGLNVEFLSIRGEIAIRTTLAGEIDMFTNAGSGIAAVARNVPVKILA
                     VLQDKPGWDLIALPNIKSIAQLRGQTVAIMSPEGSLAMVTREILKKHGIDAAKDAQLV
                     VMGGDDVRFPALKAKAIQATLFNTAASLRAQKDGFVKLAAAGEYVNTIQGGLATSDDK
                     IKQQPAKIAKIMRAALKGAAFYLAKRDAAIKYSMDILKLKDRDMAAAIYDEEAKLMVR
                     NGISDPKILQPMIDEMKAATKTQRDMKFADVFDFSFARKANEELKATGWKP"
     gene            complement(2411..>3158)
                     /locus_tag="VE170_13550"
     CDS             complement(2411..>3158)
                     /locus_tag="VE170_13550"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16514.1"
                     /translation="TSSYNRWMGQRLTGQARIKWSAVVNLDDISGAVKQVREAKKLGA
                     VSVMVLGTAGDDMLDHPRLDPFYAALCEENLALGVHVGWSCPSINNLYSHIYPSGVIA
                     FHVPLLMAFTALVSGGVLDRFPQLKVVFLEAGCQWVPFIIERIHHRFKNQGSTLRKFL
                     PQTAPIRKLPVMEYIKQGNIYFSTEVEDEILPHVLNLVGEGQVIFGSDMPHGDRDRCA
                     AAILQKRTDISDSAKERILDKNPATLYGIG"
BASE COUNT          676 a          862 c          952 g          668 t
ORIGIN      
        1 cgaagcattg attgtaggct tcgacctcgc caagaaaatt cttcaggtct tcgaaacgac
       61 actgcttgcc cagctcttcg aggctgtcgg ctttcttgat gaagggtgaa ttgtcgagca
      121 actcttccac cttcgctcgg tcgatgacat cgccgcggcg gtagtaaggg tcggcgacgt
      181 caatttttgc cagcatcggc gtatcggcga tggcccaggc gtggggcggc gtttgcgcga
      241 gcagcgccgg ggttgccgaa gcgccgccgg tgcgcgactc gttgtgaaag cgccgtccct
      301 gctggttgaa ccagatgtag ccgggcacca tgcggaaaac caaaccgcgg cgttgctgcg
      361 ggtcgcggta atccggggtg gcgtagacat agaaccagat gcagtccata tgtgtgaaat
      421 agccgccgag ctcgccaatc atcttgtggc cttcgccctt ggcgccgaaa ccggagccct
      481 ccatgacgcg aaagtttttg aactcggggc gatgttccag gaccatttcg agattggaat
      541 tgaaaccgcc ggtggcgacg acgacggttt tactgaggat gtcgatcgct gcgccacctt
      601 tggtagctgt gagaccggcg acgcggccgt ctttcgtgat cagtttgccg gctttggtcg
      661 ccgtcatgat cgtgatgtta ggaatcgatg cggcggttgc gatcagcgcg gtcatgagac
      721 ccaaaccgtt gttgtcgggg cgatgccagc gaagcacgcg gttgccttcc tgaaatttca
      781 tgtcgaccca tttgacgccg cagctctcgg cccagtgata gagctcgtgc aaactatgct
      841 cgagatagta gcgcgcccag atttcgtcgg cctggccgcc gccccacttg acccaatctt
      901 cgaatgccaa gtcgggcgta tcgttgattc cctcggcctt ttgcagcggc gtgccgacga
      961 tacagcagcc gccgccggag atcgccgccg tgccgccgca ttccgccgcg ccttccaaaa
     1021 caagcgcttt gccgccggcg cgggcgactt cgatggctgc cggtataccc gcaccgccgc
     1081 cgccgatgat cgccacatcc gtttcgatgc tcgaattagc catgaaacac tcctagggag
     1141 gacaagatag ccgagggttt atcaccgttg tccggatggg gtcaacgtca acccttgcta
     1201 agagaacgcg cattctgtat cgggaatggt gatcgcggta agcgaaggag gttatcaatg
     1261 atttgtcgcc gaatcagttt gtttcttatc gtgagttttg tcctgatgac gtttgctaac
     1321 cgaaacggac actcgcagga aaacgtccgc attgcccatc catcgttatc agcgtcggtg
     1381 ctgtgcttga tcatcgccaa caaagaaggc ttcttccgcg aagagggatt gaacgtcgag
     1441 tttctgagca tccgcggcga gatcgcaatt cgcacgaccc tggccggcga gatcgatatg
     1501 ttcaccaacg ccggcagcgg gattgccgcc gtggcgcgca atgtgccggt gaagattctc
     1561 gcggtcctcc aggataagcc gggatgggac ttgatcgcct tgccgaatat caaatcgatc
     1621 gctcagctgc gcggccagac ggtggcgatc atgtcgccgg aagggtcgct ggcgatggtg
     1681 acgcgcgaga tattgaaaaa acatggcatc gatgcggcga aggatgcgca actggttgtg
     1741 atgggcggcg acgatgtgcg tttcccggcg ctcaaggcaa aggccattca ggcgacgcta
     1801 tttaacacag cggctagtct acgcgcgcaa aaggatggct tcgtcaaact cgctgcggcg
     1861 ggcgagtacg tcaatacgat ccaaggtggc ttggcgacct ccgatgacaa gatcaaacaa
     1921 cagccggcaa aaatcgccaa gatcatgcgc gcggccttga agggcgcggc cttttatctc
     1981 gctaagcgcg acgcggcaat taagtactcg atggacatcc tgaaactcaa agaccgcgac
     2041 atggcggcgg cgatctacga cgaagaggcg aaactcatgg tgcgcaacgg gatcagcgat
     2101 ccaaagattc tccaaccgat gatcgatgag atgaaggcgg cgacgaagac ccagagggat
     2161 atgaagttcg ccgatgtctt cgacttcagc ttcgcgcgca aagccaatga ggagttgaag
     2221 gcgaccgggt ggaagccata acgggctgtc gcaaggcagc ccgcccggag cattggccgc
     2281 accgcctttt tatcgcgaag cgcgaatgtt cttattggcc cgaaggaagc gagggcatgg
     2341 aagatcacga cgctgaactt tactgctcgg gtcattcgtg gtgagaatat tcttggaatc
     2401 gtaactcaaa tcacccgatc ccatacaacg tcgccggatt cttgtccaaa atcctttctt
     2461 tcgccgaatc actaatatcc gtacgcttct gcaatatcgc ggcggcgcag cggtcgcgat
     2521 cgccgtgggg catgtcgctg ccgaagatga cttggccttc gccgaccaaa ttcaaaacgt
     2581 ggggtaaaat ttcgtcttcg acttcggtgc tgaaatagat attgccttgc ttgatgtatt
     2641 ccattaccgg cagtttccgg atcggcgcgg tttgcggcag gaattttctc agcgtcgagc
     2701 cctggttttt gaagcggtga tggatgcgct cgatgataaa cggcacccat tggcagccgg
     2761 cttcgaggaa aaccactttc aactgcggga aacgatcgag cacgccgccg ctaaccaggg
     2821 cggtgaaggc catcaatagc ggcacgtgaa aggcgatgac gccggagggg tagatatgcg
     2881 agtagagatt atttattgac gggcaagacc agccgacatg gacgcctaag gcgagatttt
     2941 cttcgcagag cgcggcgtaa aacggatcga ggcgcgggtg atcgagcatg tcgtcgccgg
     3001 cggtgccgag caccatgacc gagacggcgc cgagtttttt cgcttcgcga acttgtttga
     3061 ccgcccctga gatgtcgtcg agattgacca ccgccgacca cttgatgcgt gcttggccgg
     3121 tcaggcgttg acccatccaa cgattgtatg aagatgtg
//
LOCUS       DASQKS010000528         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2822170, whole genome shotgun sequence.
ACCESSION   DASQKS010000528 DASQKS010000000
VERSION     DASQKS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2822170"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            641..1891
                     /locus_tag="VE170_13555"
     CDS             641..1891
                     /locus_tag="VE170_13555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16515.1"
                     /translation="MSEPSDHNQPTDTVIRPWSSLLIRDYRLIFTAILCGNTSNHMRN
                     VATLYHVYQLSGSSVQLGFTGFFQAAPFIFFGLFGGVLADTLNRKKLIAITHILNIFP
                     GLLLALLTVSGSIQVWHINVLMVFAGALQVLGGPARQAIIPSLIPQSHLLNAVTLATL
                     MMQGTQLTAPVIAGFMIDFFGVAASYVVDALLPLPSVVAALMIRGSGIPQGQRRQIGW
                     HSLIEGVEFLWHTRIILSLFVLDFFAVLFGFYRPILPIFADEIYHVGARGLGMLYASP
                     AIGALIGSGILLAFGDVKRKGALSVIVTMMFALSLGLLGLSQWFWMGLIAVGLLGISD
                     AISVAMRRTVVQILAPDDMRGRATSFLTVFAQTTNATGAVIAGAGAALIGAPNAALVG
                     CVLCALTVVGTCWAIPQLWHYRSE"
     gene            complement(2090..2530)
                     /locus_tag="VE170_13560"
     CDS             complement(2090..2530)
                     /locus_tag="VE170_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16516.1"
                     /translation="MADLQVQGVVHWSIPVNNLEESEKFYGEILGLESKGRLGGSKMS
                     CFKAGSHDILLCERKEPLTRTPQQDNRLHHAFMVSPDMWDRGAKLLHDKGVKIAEAIV
                     YREKGHFTGRELYFLDPSGNMLELCDPTWKPGMPTPTYEEIVGK"
     gene            complement(2602..>3758)
                     /gene="rlmD"
                     /locus_tag="VE170_13565"
     CDS             complement(2602..>3758)
                     /gene="rlmD"
                     /locus_tag="VE170_13565"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HYJ16517.1"
                     /translation="PYQAPCRHYPNCIGCPFIDLPYPEQLVRKRALVGEALGKFAALK
                     DMQIPEVVPSPRRLGYRARVKLVVRKSGGAIATGLYVPQTHRVMDISSCPVHPRRVNQ
                     VLDYLKSRALELGIVPYDERDDSGDLRYVDFRYSFARQEVSVTLVSRHAVLPEGEKLA
                     RALQRKFSFVTGVILNINENRGNVIWGAVNRMLSGRESLLEQIGGLKLVYPAGVFSQS
                     NPNTAQKLYDHVRELANLTGKESVLDLYCGVGPISLNLAAGARQVWGIDDNETAIVTA
                     KQNARRNGVGNCRFFTGDAAALIRELGGQLGSLDLIVVNPPRKGVQAAALTALLELHA
                     PKLVYVSCEPSSLARDLEKLVQGGYRLQSVQPFDMFPQTAEVETVVELQL"
BASE COUNT          723 a         1167 c         1023 g          845 t
ORIGIN      
        1 ccatcgcgaa aataggtcgc cgcgtcgccg ggaaaatcga cgccgaaacg gctcaccgcg
       61 ccggtgcctt tttggaattc aatatggccg cgccccggat gattgacctt gtcgataaag
      121 cgcaccggcc agtaatgggt atcgccgtca atatacatgc tcacctcgtg ggaattttat
      181 tcggacctgg gacagtcacc gcagtgttgt ttgtgcaaac tatctcggcg tgcaatcgcc
      241 actccaccca tcccagggcg gatcatgatc cgcccctacg tccggacatc cttttcggat
      301 tatgatttgc gcccctttgt gttctttcag gccattctcc gaatccgtag agcgccggtc
      361 cctgtaccct cgtactacta tccgtgatag ttttctccgt ctgcgcaaat tttgtcgcca
      421 tgggctcgcc gctccgcacc tctaggtcag gagttcgtct tgaaatttta ttttgcgtcc
      481 ctttgcgttc tttgcggcca ttcttctttt cggttgcggc tacgtcgcgc ttcgacgcga
      541 ttctatggcg cgaccgccaa gagttcaaca acgtttgacc caatttagtc gaaccgatac
      601 attggccaga cgcacaaacc ttccggagcc taacaaccga gtgagcgaac cttcagatca
      661 taaccaaccc actgacaccg tcatccgccc ctggtcatcc ctgctgatcc gcgactaccg
      721 tttgatcttc acggcaattc tgtgcggcaa cacctcgaac cacatgcgca atgtggcgac
      781 cttgtatcac gtttaccaat tgtccggctc atcggtgcag ttgggcttca ccggcttctt
      841 tcaagccgcg ccgtttattt tcttcggcct gttcggcggc gtgctcgccg acacgctcaa
      901 ccgcaagaaa ttaatcgcca tcacccacat tttaaatatc tttcccggtt tgctgttggc
      961 gctgctcacc gtcagcggat cgattcaggt ctggcatatc aatgtcctga tggtctttgc
     1021 cggcgccttg caagtgcttg gcgggccggc acgccaggcg atcattccga gtttgatccc
     1081 gcagtcccac ctgctcaacg ccgtgacctt ggcgaccctg atgatgcagg gaacccagct
     1141 caccgcgccg gtgatcgccg gcttcatgat cgacttcttc ggcgtcgccg cctcctacgt
     1201 tgtcgacgcc ttgctgccgc tgccgtcagt ggtcgcggcg ctgatgattc gcggctcggg
     1261 aataccgcag ggacaacgcc ggcaaatcgg ctggcacagt ctgatcgaag gcgtcgagtt
     1321 tctctggcac acgcggatca ttctgtcgct cttcgtgctt gatttttttg ccgtcttatt
     1381 cggtttctac cggccgattc tgccgatctt tgccgatgaa atttaccatg tcggcgcgcg
     1441 cgggctcggc atgctttacg cgtccccggc gatcggcgcg ctgatcggtt cgggcatcct
     1501 gctcgccttc ggcgacgtca agcgcaaggg cgcactgtcg gtcatcgtca ccatgatgtt
     1561 cgccttgagc ctgggattgt tgggattgtc ccaatggttt tggatggggc tcatcgccgt
     1621 cgggctgctc ggaattagtg acgccatcag cgtcgccatg cgccgcaccg tcgtgcagat
     1681 cttggcgccc gacgatatgc gcggccgcgc cactagtttt ctcaccgtct tcgcccagac
     1741 caccaacgcc accggtgccg tcatcgccgg cgccggcgcc gccctcatcg gcgcccccaa
     1801 cgccgcgctg gtcggctgcg tgctctgcgc cctgaccgtc gtcggaactt gctgggcgat
     1861 cccccagctg tggcactatc ggtcggagtg aagatcgttt cgggtctaga gaatcggaga
     1921 atctcccgca aaggcgccaa gaccgcaaag gtgacggcga tgcccgtcat cccgagagaa
     1981 cacgagggat ctaagaaaag atttctccca acggtcgaaa tgacagtaag ggcgattcat
     2041 gaatcgcccc taccttggcg cgccttagcg ggcggaatat ccgaatcact tatttcccga
     2101 cgatctcttc ataggtcggc gtcggcatgc ccggcttcca ggtcggatcg cagagttcca
     2161 acatgtttcc gctcggatcg agaaagtaca attcgcggcc ggtgaagtgg cccttctcgc
     2221 gatagacgat cgcttcggca atcttgacgc ccttgtcatg caataatttc gcgccgcggt
     2281 cccacatgtc ggggctgacc atgaacgcat ggtgcaagcg gttgtcttgc tgcggtgtgc
     2341 gggtcaaagg ctccttgcgc tcgcacagca gaatatcgtg gctgccggct ttgaagcagg
     2401 acattttcga accgcccaaa cgccccttcg actcaaggcc gagaatctcg ccgtaaaact
     2461 tctccgattc ctccaaatta ttaaccggga tcgaccaatg aaccacacct tgaacttgta
     2521 aatcagccat tagggaacct ccaaataaat ttaatttgca ccatcaaagc atgcgcgcga
     2581 tgaaaatccc aataaccgcc gtcaaagctg cagctctacc accgtctcta cctccgcggt
     2641 ctgcggaaac atatcgaaag gctgcaccga ttgtaggcga taacctccct gcacgagttt
     2701 ttccaaatcg cgcgccagac tgcttggttc gcaggagaca tagacgagct ttggcgcgtg
     2761 cagttccagc agcgccgtta gagcggcggc ctgcacacct ttgcgtggcg gatttacgac
     2821 gatcagatcg agcgaaccta gttgaccgcc cagctcccgg atcaacgcgg cggcgtcgcc
     2881 ggtgaaaaac cggcagttgc cgacgccgtt gcgccgggca ttttgcttcg cggtgacgat
     2941 ggccgtctcg ttatcgtcga tgccccatac ctggcgcgcc ccggccgcga ggttgagcga
     3001 gatcggtccg acgccgcagt agagatcgag cacggattct ttgccggtca aattggctag
     3061 ctcgcgcaca tggtcgtaga gtttttgcgc cgtattggga ttggactggg aaaacacccc
     3121 ggcgggatag accagcttta gccccccgat ctgctcgagt aacgattcac ggccggagag
     3181 catgcggttg acggcgcccc agatcacatt gccgcgattt tcattgatgt tgagaatcac
     3241 cccggtaaca aatgaaaatt ttcgctgcag cgctcgcgcc agcttttcac cttcgggcaa
     3301 taccgcatgg cggcttacca aagtcacgct gacctcctgg cgggcaaagc tatagcgaaa
     3361 gtcgacatag cgcagatcgc cgctgtcgtc gcgctcgtcg tagggaacga tccccaattc
     3421 cagtgcccga ctcttcagat aatccagcac ttgattgacc cggcgcggat gcaccgggca
     3481 tgacgaaata tccatcaccc ggtgggtttg cgggacgtag agtccggtgg cgatggcgcc
     3541 gccgctcttg cgcacgacca gcttgacccg ggcgcggtaa cccaggcggc gcggcgacgg
     3601 cacgacttcg ggaatttgca tatccttcag cgcggcgaac ttgcccaacg cctcgccgac
     3661 cagcgcgcgt ttacgaacca gctgttcagg atagggcaga tcgataaacg gacagccgat
     3721 acagttggga tagtgccggc acggcgcctg gtacgggc
//
LOCUS       DASQKS010000529         4582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3241037, whole genome shotgun sequence.
ACCESSION   DASQKS010000529 DASQKS010000000
VERSION     DASQKS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241037"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VE170_13570"
     CDS             <1..578
                     /locus_tag="VE170_13570"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYJ16518.1"
                     /translation="RGEVIVFPTETLYGLGADALNFSAVENVFHLKGRDPDNPLPVLV
                     ADRIMLDSLVVKVSPLAEKLMGHFWPGPLTLVLPARDDIPRPLVNANGGIGVRVSSQP
                     IATQLVRLLGHPLTATSANPSGQPGAHTVVEAKNYFSAKITVYIDGGELTSPTGSTVA
                     AVEKNKLRIIRAGEISREALEVVVGKGVIEA"
     gene            575..793
                     /locus_tag="VE170_13575"
     CDS             575..793
                     /locus_tag="VE170_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16519.1"
                     /translation="MKFGIRGMAPLAIAICLLGGCDANKAPVEKGREAVKEVITQPFN
                     ALDSAKDSLKQSDDKQKAALEETNKEIK"
     gene            1008..1985
                     /locus_tag="VE170_13580"
     CDS             1008..1985
                     /locus_tag="VE170_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ16520.1"
                     /translation="MLVLSEKQVQSLIDIDEIIAALEQAHIQYSTGKAVMPVRLVVPL
                     PQIQGRITSMPGYLNEDKALGMKVVTYFQDNPKQNLPAILATIMLFSSDTGKMIAAMD
                     GGYITAIRTACASALATRILANPSTPVLGILGAGVQARAHIQALSRVRQFSHIKLYSP
                     SGKSALGIKAALEKPDGIPIEVVDSAQAAVRDSDLLVTVTTAKDPIVQVEWLKPGVHI
                     NAVGSHRPDLREIDGATLKRAKVVVDSREAIMAECGDILLALKEQSIGADDIHGEIGQ
                     VLAGTKAGRISPSEVTLYKSVGIAIQDVATAQMVYRKALAQNVGTTVEI"
     gene            2091..3845
                     /locus_tag="VE170_13585"
     CDS             2091..3845
                     /locus_tag="VE170_13585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HYJ16521.1"
                     /translation="MVQFKAIIRADVAALQQTAVSLPTLTTQWANFWEDILLRNEDLS
                     RRMSHLGSYIGCLASSDAGNESYQKEEAEITRTRAELAKVRIELLRAFKATEDDVFAE
                     FCARPALAGAQNYLTRLREEARRAMTAEKEILATDLSVDGIQAWGRLYDRVSAKLEFS
                     MVYPDGRRARVPMSQRRSLMDDPDRRVRQAAFEGGNAAWQSVEDTAAAALNAIAGTRL
                     TLNRHRGVDHFLDIALFQAAIGRKTLDAMFEALIANLEIPRRILQLKAQSMARQQIAW
                     FDLGAPLDLADQGKLTWDKAQATVAQSFSRAYPALGRFFTEQVIGKNWVDWEPRQGKR
                     PGGFCTSSMLSKESRIFMTYNESLGDVLTLAHESGHAYHGAMMRDIRPYARGYPMTLA
                     ETASTFGENVLMNGILDDPAVSDNDKARILDIEVGHGAVYLLDIPVRYEFEKSFYEER
                     ANGPLSVSRLKELMVQTQRRVLGDVLETGGEDPYFWASKLHFYIAGITFYNFPYTFGY
                     LLSRGLYAMFRNEGDGFLPKYEEFLRRAGSDTAENVVRQTIGRDIEKTEFWSEAIQSL
                     EEPLSQLQALLPKLTMQN"
     gene            complement(4046..>4582)
                     /locus_tag="VE170_13590"
     CDS             complement(4046..>4582)
                     /locus_tag="VE170_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HYJ16522.1"
                     /translation="EAEVRGLLGTAIFAADDETMEVVTGQLLREKNQTVAACEDLTSG
                     QLAERLQIAGNESFGAGFICNNENALRSLLKNARSGINLDATLKDPTALTDEMAWAAR
                     QQSGCDFGLALHAIADPDSKIQNLARGQTYVSLTDGKTFLRRESTSAGRGAYDRSRMT
                     LSAIDLLRTALLEGMNQK"
BASE COUNT         1044 a         1369 c         1277 g          892 t
ORIGIN      
        1 agcgcggtga agtgatcgtt tttcccaccg aaacacttta cggtctcggc gccgatgcgc
       61 tgaatttttc cgccgtcgaa aacgtctttc acctaaaagg ccgcgatccc gacaacccat
      121 tgcccgtact cgtcgccgat cggatcatgc tcgattctct ggtggtgaaa gtttccccgc
      181 tggcggaaaa attgatgggg cacttctggc cggggccgct gacgttggta ttgcctgcgc
      241 gcgatgatat tccgcgcccg ctggtcaacg ccaacggtgg catcggcgtg cgcgtgtcga
      301 gccagcccat cgccacgcaa ctggtgcgct tgctcggcca tccgctgacc gccaccagcg
      361 ccaatccctc cgggcaacca ggcgcgcaca ccgtcgtgga agccaaaaac tatttctccg
      421 ctaagatcac cgtctacatt gacggcggcg aattgacttc gccaaccggc tccaccgtcg
      481 cagcagttga aaaaaataag ctgagaatca ttcgcgccgg cgagatcagc cgggaagctc
      541 tcgaagttgt cgtgggcaaa ggagtcattg aagcatgaaa ttcggtattc gcggaatggc
      601 gccgctggcg attgcgatct gcctgcttgg cggttgcgat gcgaacaaag ctcccgttga
      661 aaagggccgc gaagcggtga aggaagtaat cacgcaaccg ttcaacgcct tggacagcgc
      721 caaggactcg ctcaagcaga gcgacgacaa acaaaaagcc gccctcgaag agaccaacaa
      781 ggaaatcaag tagaccgcgc caccccctcc agggactgtt tcgcaccgca gaaatcgctc
      841 attgctgtca tcccgagctt agcgaggggt ctcgtctttt ctgccactta cgaaggcgag
      901 attcctcggc ttcgcctcgg aatgacagtt acgagacggt ctcccactgg gaaagtcggg
      961 ggcatgagcc tacattcagc gcgcgcttta tgctatcaaa atgaatcatg ctggttttgt
     1021 ccgagaaaca agttcagagt ttgatcgaca tcgacgaaat catcgccgca ctggaacaag
     1081 cgcatatcca atattccacc ggcaaggcgg ttatgccggt gcgcttggtc gtgccgctgc
     1141 cgcagatcca aggacgcatc accagcatgc cgggatatct gaacgaggac aaagccctcg
     1201 gcatgaaagt ggtgacctat tttcaagata acccaaaaca aaatctgccg gcgatccttg
     1261 ccaccatcat gttgttcagc agcgataccg gcaaaatgat cgccgccatg gacggcggct
     1321 atataactgc aatcagaacc gcctgcgcct cggcgctggc gacgagaata ttagccaatc
     1381 ccagcacacc ggtgctggga atcttaggcg ccggcgtgca agcgcgcgcc catatccagg
     1441 cgctctctcg ggtgcggcag tttagccata tcaagctcta cagcccttcg ggaaagagcg
     1501 ccctcggtat taaagcagcc ttggaaaaac ccgacggcat tccaatcgag gtcgtcgatt
     1561 cggcgcaagc tgcggttcgt gatagcgatc tcctggtcac ggtaaccacc gccaaggatc
     1621 ccatcgtgca ggtcgaatgg ctcaagcctg gcgtccacat caacgccgtc ggctcgcacc
     1681 gtcccgatct gcgtgaaatc gacggcgcga cgttgaaacg cgccaaagtc gtggtcgact
     1741 cgcgcgaagc gatcatggca gagtgtggcg acatcttgct cgcgctcaaa gaacagtcca
     1801 tcggcgcaga cgatatccat ggcgagatcg gccaagtcct cgccggcacc aaggcaggac
     1861 gcatcagccc gagtgaagtc acgctctata aatcggtcgg catcgccatc caagatgtcg
     1921 ccaccgcgca gatggtttat cgcaaggcgt tggcgcaaaa cgtcggcaca actgtggaga
     1981 tttaatctgc cgctgcacac aacggacacg ggatttcgaa accgacccta tatcggaact
     2041 ctatgacctg ggacctgact agttactttg ctgagtttaa cggcgccgcg atggttcaat
     2101 tcaaagccat catccgcgcc gatgtcgcgg cgctgcagca aaccgccgtg agcttgccga
     2161 cactgaccac gcaatgggcc aacttctggg aagatattct actgcgcaac gaagacctgt
     2221 cgcgccgcat gtcacacctc ggttcctaca tcggttgctt ggcttcatcc gacgccggca
     2281 acgagagtta ccaaaaagaa gaagcagaga taacccgcac ccgcgccgag ctcgctaaag
     2341 tcagaatcga actgttgcgc gccttcaaag ccaccgagga cgacgtcttt gccgagttct
     2401 gcgcacgtcc cgcattggcg ggagcgcaaa actatctgac aagattgcgt gaggaagcgc
     2461 ggcgcgcgat gacggcggaa aaagaaattc ttgccaccga tttgagcgtc gatggcatcc
     2521 aggcttgggg ccgcctctat gatcgcgtct ccgccaaact agaattctcc atggtctatc
     2581 ccgacggcag gcgcgcgcgt gtgccgatgt cacaacgccg ctcattgatg gacgatcccg
     2641 accggcgtgt gcgccaagcg gccttcgaag gcggcaacgc ggcttggcaa agcgtcgaag
     2701 acaccgccgc cgccgccctc aacgccatcg ccggcaccag gctgacattg aatcgccacc
     2761 gcggcgtcga tcactttctc gacatcgcgc tttttcaggc ggcgatcggg cgcaagaccc
     2821 tcgacgccat gttcgaggcg ctcatcgcga acctggagat accgcggcgc attctacaac
     2881 taaaagccca atcgatggcg cgccaacaga tcgcctggtt cgatctcggc gctcccctcg
     2941 acttggcgga ccaagggaaa ctgacttggg acaaagccca agccacagtc gctcagtcgt
     3001 ttagccgcgc ctacccggcg ctgggaagat tcttcaccga gcaggtcatc ggcaagaact
     3061 gggtcgactg ggagccgcgc caaggcaaac ggcccggcgg cttttgcacc agttccatgc
     3121 tgagcaagga atcgcgcatt ttcatgacct ataacgaatc attgggcgat gtcctgaccc
     3181 tcgctcatga atcgggccac gcctatcacg gcgcgatgat gcgcgatatc cgcccctacg
     3241 cgcgcggcta tccgatgacc ttggcagaaa ctgcgtcgac ctttggcgaa aacgtcttga
     3301 tgaacggtat cctcgatgac ccggcggtca gcgacaatga caaagcgcgt atcctcgaca
     3361 tcgaagtcgg tcatggcgca gtctacctgt tggacattcc ggtgcgctac gagtttgaaa
     3421 agtctttcta cgaagaacga gccaatgggc cgctcagcgt gtcccgacta aaagaactga
     3481 tggtgcagac gcaacggcga gttttgggcg acgtgcttga aaccggcggc gaggatccct
     3541 acttctgggc gtctaagctg catttctata tcgcgggcat caccttctac aatttcccct
     3601 acacattcgg ttatctactc agccgcggcc tctacgccat gtttagaaac gaaggtgacg
     3661 ggtttctgcc taaatacgaa gaattcctgc gtcgcgccgg cagcgacacg gcggagaatg
     3721 tcgtgcgaca gacgatcggc agagatatcg agaaaactga attctggagc gaagcgatcc
     3781 agagcttgga agagccactt agtcaactgc aagcgctttt accgaaactg actatgcaaa
     3841 actagtgggt aagagtcccc aagagcctgg agcggcacag caaccgaaaa gaagcatggc
     3901 cgcaagaaac gcaacgggcg taaaacggga ttacaagggg cgggtttgaa acccgccttt
     3961 tcctcggaac ccatcgtgac cttcgagtta ctccgggtaa acaacggcag agcgagacac
     4021 gcctcgaaca accggaacgc agcaactatt tctgattcat cccttccaac agcgccgttc
     4081 tgagcaaatc gatggcgctg agcgtcatgc gcgagcggtc ataggcgccg cggccggcgc
     4141 tggtgctttc acgcctgaga aaagtcttgc cgtcggtaag tgaaacatag gtctgcccgc
     4201 gcgcaagatt ttgtattttg ctgtcggggt cggcgatggc gtgcaaggca aggccgaaat
     4261 cgcagccgga ctgttgtcgc gccgcccagg ccatttcgtc ggtaagcgcg gttggatctt
     4321 ttaaggtcgc gtcgagattg atgccgctgc gcgcgttttt cagtaatgag cgcagcgcat
     4381 tttcgttatt gcaaataaag ccggcgccga agctctcatt gccggcgatc tgcagccgct
     4441 ccgccagctg gccggaggtg agatcctcgc acgcagctac tgtctggttt ttttcgcgca
     4501 gcaattgccc ggtgactacc tccatggttt cgtcgtcggc ggcgaaaatt gccgtgccca
     4561 acagcccgcg cacctcggct tc
//
LOCUS       DASQKS010000530         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3667596, whole genome shotgun sequence.
ACCESSION   DASQKS010000530 DASQKS010000000
VERSION     DASQKS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3667596"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VE170_13595"
     CDS             <1..1367
                     /locus_tag="VE170_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ16523.1"
                     /translation="QQHAVHKGIDVYMECNLQRLLTDGGRVSGAFGYWRESGKFIMFK
                     CKAVVLATGGGGKAWKVTSNSWEYTGDGVTMAADAGADLMDMEFMQFHPTGMVWPPSV
                     RGILVTEGVRGDGGTLKNTKNERFMFNYIPDFFKAETAETIEEAERWYDDKRNNRRTP
                     DLLPRDEVARAINSEVKAGRGTPHGGVFLDIATRRPADYIRKRLPSMYHQFKELADVD
                     ITKEAMEVGPTCHYSMGGVRVDADSTATTVPGLFAAGEVAAGLHGANRLGGNSLSDLL
                     VFGRRAGLYAAEYAKGLSGSLSVNNDQVDQFAQTMLAPFETQGNENPYTIHSDLQECM
                     QDLVGIIRVESELNQALEIIQKLKQRSLNVKVTGNRHYNPGWHLSLDLHSLLTFAEAA
                     TLAAIIRKESRGGHTRDDFPNPDPKFAKVNIITRIHNGEMTQSQEPLPEMPGELKALL
                     EEKQ"
     gene            1367..2122
                     /locus_tag="VE170_13600"
     CDS             1367..2122
                     /locus_tag="VE170_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HYJ16524.1"
                     /translation="MAEQQVHMRVWRGDANGGEFKDYRIAVGEGQVVLDVIHAIQAQQ
                     ANDMAVRWNCKAGKCGSCSAEVNGKPRLMCMTRMNTFEPGETITVAPIRTFPIIRDLV
                     TDVSYNYQKAKEIPAFKPKPKEADGTRRMYQEDIDRVQEFHKCIECFLCQNVCHVIRD
                     HPENKPAFSGPRLFVRLAALEMHPLDTNDRMELIKQKAGLGYCNITKCCTEVCPEHIH
                     ITDNAIIPLKERVVDRYYDPIAKLMGLFSAKNK"
     gene            complement(2225..2743)
                     /locus_tag="VE170_13605"
     CDS             complement(2225..2743)
                     /locus_tag="VE170_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16525.1"
                     /translation="MAEIASYSQQFRALGQQLEDLGMERFTVRVEADGYRVTGTKRYQ
                     PPAEEQPPKKLWQRIRSGAKSAGSTPQPFEPVALHFSAQDLAHIDVDAKHKRSGTPGN
                     TDAHSLSQVFRAVGAIVEQKQGRFLSVIKEGQEIVIEYESISKRNTVEKFTVSSLYDY
                     WVKMYLRRRERS"
     gene            complement(2962..3459)
                     /locus_tag="VE170_13610"
     CDS             complement(2962..3459)
                     /locus_tag="VE170_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16526.1"
                     /translation="MDFKSGYAHTLRAIGQALETLDLGDFSMEPAGDGYHVTGASVTK
                     YPGLVRNTGSERGARNPSVRMTPIVLNYSPGDVRRLEREGQTQRVETHGMTDTSRLSQ
                     ALRAIGFYLSQKYSRMVRLSRRGESFEVIYESSLGNRYSEYFTAADLYNLWVRFYLQR
                     SARCA"
BASE COUNT          894 a         1132 c         1049 g          792 t
ORIGIN      
        1 tccaacaaca cgccgtccat aaaggcatcg atgtctacat ggagtgcaac ctgcaacggc
       61 tattgaccga cggcggccgc gtcagcggcg cgttcggcta ctggcgcgaa agcggcaagt
      121 tcatcatgtt caagtgcaaa gccgtcgtgc tcgccaccgg cggcggcggc aaagcctgga
      181 aagtcacgtc gaattcttgg gaatacaccg gcgacggcgt caccatggca gccgatgccg
      241 gcgccgattt gatggacatg gagttcatgc agtttcatcc gacgggaatg gtctggccgc
      301 ccagcgtgcg cggcattctc gtcaccgagg gcgtgcgcgg cgacggcggc acattgaaga
      361 acaccaagaa cgaacggttc atgtttaatt acatccccga tttcttcaaa gccgagaccg
      421 ccgagactat agaggaagcc gaacgctggt acgacgacaa acgcaacaac cgccgcaccc
      481 ccgatttgct accgcgcgac gaagtcgccc gggcgatcaa ctcggaagta aaagccggcc
      541 gcggcacgcc ccacggcggg gtcttcctgg acatcgctac acgccgtccg gccgattaca
      601 tccgtaagcg gctaccgtcg atgtatcatc agtttaaaga actggcggat gtcgacatca
      661 ccaaggaagc gatggaagtc ggcccaacct gccactattc catgggcggc gtgcgtgtcg
      721 acgccgacag cacggcgacc acggtccccg gtttattcgc cgccggcgaa gtcgccgccg
      781 gtctccatgg cgccaatcgc ctgggcggca attcgctctc cgatttgctg gtattcggtc
      841 gtcgcgccgg tctttatgcc gcggaatacg ccaagggcct gagcggatcg ttgagtgtaa
      901 ataacgacca ggtagaccag ttcgcgcaaa ccatgctggc gccctttgaa acccaaggca
      961 acgagaatcc ttacacgatt cattccgacc tgcaggagtg catgcaggac ttggtcggca
     1021 tcattcgcgt cgagtcggaa ttgaatcagg cgctggaaat tattcaaaag ctcaagcagc
     1081 gctccctgaa cgtaaaagtc accggcaacc gccactacaa tccgggttgg catttgtcat
     1141 tggatctgca ttccctattg accttcgccg aagcggctac gttggcggca attatccgta
     1201 aagaaagccg cggcggccac acgcgcgacg acttccccaa ccccgatccg aaattcgcca
     1261 aggtcaacat catcacgcgc attcacaatg gcgaaatgac ccagagccag gaaccgctgc
     1321 cggaaatgcc cggcgaacta aaagcgctgc ttgaggagaa acaataatgg ccgagcaaca
     1381 agtccatatg cgggtgtggc gcggtgacgc caacggcggt gagttcaaag actaccgcat
     1441 tgccgtgggc gaaggacagg tggtgctcga cgtcatccat gcgatccagg cgcagcaagc
     1501 caacgacatg gcggtgcgtt ggaactgcaa agccggcaaa tgcggctcat gcagcgctga
     1561 agtcaacggc aagccgcggc tcatgtgcat gacgcgcatg aacacgttcg agcccggcga
     1621 gaccatcacg gtagcgccga tacggacctt cccgatcatc cgcgatctcg tcaccgacgt
     1681 gtcgtataac tatcaaaagg ccaaagagat cccggcgttc aaaccgaagc cgaaagaagc
     1741 cgacggcacg cgccggatgt atcaagaaga catcgatcgg gtacaggaat ttcacaagtg
     1801 tatcgagtgc ttcctttgcc aaaacgtctg ccatgtcatc cgcgatcacc ccgagaataa
     1861 gccggccttc tccggcccgc gcttattcgt ccggttggca gccttggaaa tgcaccccct
     1921 cgacaccaat gatcgcatgg agctgatcaa acagaaggcc ggcttgggct actgcaacat
     1981 caccaaatgc tgcaccgaag tctgcccgga acatattcac atcaccgata acgctatcat
     2041 cccgctcaag gaacgcgtcg tcgaccgcta ctacgaccct attgccaagt tgatgggact
     2101 cttctccgcg aagaacaaat aactctcgga acgcggcgcg caacagcctc gaatctgcgc
     2161 gccgcgtcgg gccgacaaac actattcaag gacgccgcat ggccgcggct gttgattggg
     2221 ctgcctagct gcgctctcgg cggcggagat acattttcac ccagtagtca tagagcgacg
     2281 agacggtaaa tttctcaacg gtatttcttt ttgagattga ctcgtattcg atgacaatct
     2341 cttgaccttc cttgatcacc gagagaaatc gcccctgctt ttgttcgaca atggcgccga
     2401 cggcgcggaa aacttgcgac agcgaatgag catccgtatt gcctggcgtt ccgctccgtt
     2461 tgtgcttggc gtcgacatct atatgcgcta ggtcttgcgc cgagaaatgc aatgcgaccg
     2521 gctcgaacgg ttgaggagtg cttcccgcag atttagcgcc gctgcggatt cgctgccata
     2581 atttttttgg cggttgctcc tcggctggcg gttggtaacg cttggtgccg gtaacccgat
     2641 agccatccgc ctccacgcgc acggtaaaac gctccatgcc gagatcttcc aactgttgac
     2701 cgagtgctct gaattgttgc gagtagctcg ctatttcagc catctttatt taatccggga
     2761 atcaatcatc gccacggttt cgggaaaatc attcgacccg aacagagtgc aaaactttag
     2821 ctcgaaatcg ccggcaacac cgagaatctg cggcaatgag ttcgcttcgg tcgcctgacc
     2881 ggcgcggcgc cgtctgcgtg tagatgagaa gacccattac acgtctgtgc gcttatgcct
     2941 acccggcggg gtcgacccaa gctaggcgca gcgggcagac ctttgaagat aaaaccgtac
     3001 ccaaagattg tagagatcgg ccgctgtaaa gtattcacta taacggttgc cgagggaact
     3061 ctcgtaaatc acttcaaagc tctcacccct tcgggagagc cgtaccatgc gggaatattt
     3121 ttggctgaga taaaagccga tggcgcgaag cgcttgggac aatcttgagg tatcggtcat
     3181 gccatgggtc tccacccgtt gggtctgccc ttcccgctcc aaccgtcgga catcgcccgg
     3241 agaatagttc aaaacgatcg gcgtcatgcg gacgctggga tttctagccc ctcgctcgct
     3301 gcccgtgttt ctaaccaacc ccgggtattt agtaacggag gcgccggtga cgtgatagcc
     3361 atcgccagcc ggttccatgc taaaatcgcc gagatcgagg gtttcgagcg cttgcccaat
     3421 cgctcgcagc gtatgcgcat agccgctctt aaaatccaca tttccatgcg atttttctgg
     3481 gaacggtaga tccgctatcc tcacggcgcc tcccgattta tcaaaaattc ttaagctaga
     3541 aattattttc taatagcaac ttatcaattt gtcaaggtaa actgtcccac ccttaaagcg
     3601 aacgaatgag ccacaacaaa ttttgcgaac ggagtgagtg gacggacgcc tgggggaaat
     3661 ggcaagtggc gagtgactgt tgttatagtg ccggtccgac tcgtcaccgg gaggttcggg
     3721 aagaccaaag tcccgaatat tagtttctgc gcaaacgaaa cgcgcccgct gcctgcatgg
     3781 cctcagtctt ggggctttcg cccgcctcgg cgattggctc ttcatcgacc ttaagccctg
     3841 cgagcttgat cttcaggcgt aaattgt
//
LOCUS       DASQKS010000531         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6179389, whole genome shotgun sequence.
ACCESSION   DASQKS010000531 DASQKS010000000
VERSION     DASQKS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6179389"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..933)
                     /locus_tag="VE170_13615"
     CDS             complement(76..933)
                     /locus_tag="VE170_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ16527.1"
                     /translation="MTRSLTILAVAYFLMALTDLGHAQNTLEKIDKSGTLIIGTRNGS
                     PPFAYINKNNDWVGFSIDLVEQTVLPALSKKLNKQIKLEKKESAPATRIPLLTSNAVD
                     LIAETMTDTQSRREQVDFSLTFFVTGAQFLVRKGSPVKNIESIAGKRIAAQQGSTNAK
                     IIRERVPSAKLVEFPDQPAAFQALSQRQVDAYTNDGIQLAGLKAKAPKPDEWVVAGEF
                     FSYEPYGMAMRKNDSDFRQVVNVGLMDAIASGRYAEIYEKWFGAKGEVPYPLTPENKR
                     FLQLQVVPK"
     gene            complement(945..1673)
                     /locus_tag="VE170_13620"
     CDS             complement(945..1673)
                     /locus_tag="VE170_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HYJ16528.1"
                     /translation="MIEFSHVNKWFGALHVLSDIDLTIAAGNVVVVCGPSGSGKSTLI
                     RCINRLETIQSGEIVVDGMPLSDPKLDAAKLRANVGMVFQSFNLYPHMTVLQNIALAP
                     VKVKNIAAAEAESTAKQLLERVGIPDKANVYPANLSGGQQQRVAIARALAMKPKVMLF
                     DEPTSALDPEMINEVLEVMTDLAKDGMTMVVVTHEMGFARRVAHRVVFMDQGKIVETA
                     EPETFFAAPQSARAQQFLSKILSH"
     gene            1851..2297
                     /gene="sixA"
                     /locus_tag="VE170_13625"
     CDS             1851..2297
                     /gene="sixA"
                     /locus_tag="VE170_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HYJ16529.1"
                     /translation="MDCYLMRHGETLSGVPDGRRALSERGRSAVTALAQRAMARGVVV
                     AWIYHSGILRAQQTAELVAEVLTPAAGVRQLAGLCPDDDPFIVQAELAAASQPIMLVS
                     HLPYIGRLASLLTSGDSGGSMIEFAPATLVCLTRHTGGWKLSWSMR"
     gene            2371..3657
                     /locus_tag="VE170_13630"
     CDS             2371..3657
                     /locus_tag="VE170_13630"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HYJ16530.1"
                     /translation="MAAETLTIVDNRTGKQYEIPINHGAIKATDLFQIKISEDEGIVS
                     YDPGFMNTASCQSKITYIDGDKGILRYRGYPIEQVAENSTYLETAYLILNGELPTQAQ
                     LDDFTHHVTFHSIIHENIKKLIDGFHHDAHPMGMLVSTVGALSTFYPNAKKIFDAQNR
                     REQTYRLVGKMPTLAAFAYRHSLGLPYSYPDNDLSYTGNFLNMLFKMTELKYKPNPVL
                     ERALDILFILHADHEQNCSTNAMRGIGSSQPDPYSSVAGAAAALYGPLHGGANEAVLR
                     MLMEIGSKDKVPEFIQKVKGGSGRLMGFGHRVYKNYDPRARIIKNIADQVFDVMGKNP
                     LLEIALELERIALSDDYFIKRKLYPNVDFYSGLIYQSMGLPMDMFPVLFAIPRTIGWI
                     AQWEEMLTDPDQKLARPKQIYLGAASRDFVEMSKRK"
     gene            complement(3661..>4919)
                     /locus_tag="VE170_13635"
     CDS             complement(3661..>4919)
                     /locus_tag="VE170_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYJ16531.1"
                     /translation="SNAPTTPQIDIAINVADAGEAIAVPPMPRPQPAPLAPPVEMKNH
                     RVEKLEKAAPTIGSAANPIVTARPAENDNRLLEFLDKDIDKAIEQPKERRHLHFSKAV
                     VDHPRVRQFVRQYSVTNKVYFETLLARSGRYMPIIAKVLSEEGLPEELAYLALLESEF
                     QVNSTSPAGAVGLWQFVASTARQYGLRIDDWVDERRDPVKSTRAAAAYLKDLHQYYGR
                     WYLVTAAYNAGSGIINKALQTSRASDFWDIKEKAQLSQETRNFVPKFVAIAMIATNPK
                     KYGFNQLRYDEPLEFDEVDAEGLVKLDAAAEMAETDIAMIKELNPALLRNITPPGSRG
                     YRLRVPVGRALVFAKASEYQRSKEPESVQLVTHEVKRGDTLASIAARYGQAISALMEL
                     NGLTTSQLQIGQKLRVIFEGLRGTLR"
BASE COUNT         1032 a         1309 c         1424 g         1154 t
ORIGIN      
        1 ggagaattag agtatcggag tgatggagta ttggagtaat ggtcgtaaaa cccatactcc
       61 atcactccag tttttttatt tgggcaccac ttgcagttgc aggaagcgtt tgttttccgg
      121 tgtcaacggg tagggcactt cgcctttggc gccgaaccat ttttcgtaaa tttccgcgta
      181 gcggccggaa gcgatcgcat ccattaagcc gacgttgacg acttggcgaa agtccgaatc
      241 gttcttgcgc atcgccatgc catagggctc gtatgaaaaa aactcgccgg ctaccaccca
      301 ttcgtccggt ttgggggcct tggctttcaa tccggcgagc tggatgccgt cgttggtgta
      361 ggcgtcgact tggcgctggg acaaagcttg gaaggcggcg ggctgatcgg ggaattccac
      421 cagcttggcg ctggggacgc gctcgcgaat tattttggcg ttggtcgagc cttgctgcgc
      481 ggcgatacgt ttgccggcga tgctttcgat gttcttcacc gggctacctt tgcgcaccag
      541 gaattgcgcg ccggtgacaa agaaggtcaa actgaagtca acctgttcgc gccgcgattg
      601 ggtatcggtc atggtttcgg cgatcagatc gacggcgttc gaggtaagaa ggggaatgcg
      661 ggtcgcgggc gccgattcct ttttttccag tttaatttgt ttgttcaact tcttgctcaa
      721 ggccggtaga accgtctgct cgactagatc gatggaaaac ccaacccagt cattgttctt
      781 gttgatgtac gcgaacggtg gtgacccgtt gcgcgtgccg atgatcagtg tgccgctctt
      841 gtcgattttc tccagggtgt tttgcgcgtg gcctaggtcg gtgagggcca tgagaaaata
      901 ggcgaccgcg aggatcgtta acgaccgtgt cataaatttg ctccttagtg cgaaagtatt
      961 ttgctcaaga attgctgtgc acgcgccgat tgcggagcgg cgaaaaatgt ttccggttcg
     1021 gcggtctcga cgatcttccc ctgatccatg aagacgactc ggtgagcgac gcgccgggca
     1081 aaacccattt cgtgggtgac caccaccatg gtcataccgt ccttggccag atcggtcatg
     1141 acttcgagca cttcattgat catctcggga tcgagcgccg aggtcggctc gtcgaagagc
     1201 atgaccttgg gtttcattgc cagggcgcgg gcgatggcga cgcgttgctg ctgtccgccg
     1261 gatagattgg ccggatagac gttggctttg tcgggaatgc cgacgcgctc caacaattgc
     1321 ttggcggtcg attctgcttc ggcggcggcg atgtttttaa ctttgaccgg tgccagagcg
     1381 atgttctgca ataccgtcat gtgcggatag agattgaacg actggaatac catgccgaca
     1441 ttggcgcgta gcttggcggc gtcgagctta gggtcggaca gtggcatgcc atcgacgaca
     1501 atctcgccgg actggatggt ttccaatcga ttgatgcagc ggatcaaggt gctcttaccg
     1561 ctgccgcttg ggccgcagac caccactaca tttcccgcgg cgatggtgag gtcgatgtcg
     1621 ctcaaaacat gcagcgcgcc gaaccatttg ttgacgtggc tgaattcgat cacggttatg
     1681 cagacttgcc caaattcgat tgcaaaacgc tggtcggcat actatatcaa catcggcttt
     1741 ttgcaagaat aataagcgct tataacgatc tcgccgataa tatcgtaaag caccaagcaa
     1801 caaaacgatt gtgcgcgttg tcaagcggca tagcgaggaa ttagcaagcg gtggactgtt
     1861 atctgatgcg tcatggggaa accctttcgg gtgtccccga cgggcgacgg gcgttgtcgg
     1921 aaagagggcg tagcgcagtg acggcgctgg cgcaacgcgc catggcgcgc ggcgtggtcg
     1981 tcgcttggat ctaccattcc ggtatcttgc gggcgcaaca gactgctgaa ctggtcgccg
     2041 aagtcctgac tccggcagcg ggcgtgcgcc aactagccgg gctatgtccc gacgacgatc
     2101 catttatcgt ccaggccgag ttggcagcgg cgagccaacc gatcatgctg gtgagccact
     2161 tgccatacat cggtcggctc gccagtttac tgaccagcgg tgatagcggc ggctcgatga
     2221 tcgagtttgc gccggccact ctggtttgcc tgacccggca cacaggtggg tggaagctca
     2281 gctggtcgat gcgctgagcg cagttgaccg gcgcatgacg atggtctaat aaaaggctag
     2341 agtgcaaaca gtggtgaaag gtagattcca atggccgcag agacgctaac gattgtcgac
     2401 aaccgaaccg gaaagcagta cgagatccca atcaaccacg gcgccatcaa ggcaacggat
     2461 ttgttccaga tcaaaatctc cgaagacgaa ggtatcgtca gctacgaccc tggcttcatg
     2521 aacacggcgt catgtcaaag caagattacc tacatagacg gtgacaaagg catattgcgt
     2581 taccggggct atccgatcga acaggttgcc gagaatagca cctacctgga gaccgcctat
     2641 ctgattctca acggcgagct gccgacccag gcgcagttgg acgacttcac ccatcatgtg
     2701 acttttcact cgatcattca cgaaaatatc aaaaagctca tcgacggttt tcatcatgac
     2761 gcccatccta tgggcatgct ggtaagcacg gtgggcgcgt tgtcgacgtt ttatcccaac
     2821 gcgaaaaaaa tctttgacgc gcagaatcgc cgcgagcaga cgtatcgttt ggtcggcaaa
     2881 atgccgactt tggcggcctt cgcctaccgc catagcctcg gcttgcctta ctcgtacccg
     2941 gacaatgact tgagctacac cggtaatttt ttgaacatgc tattcaagat gactgagctc
     3001 aagtacaaac ccaacccggt gctcgaacgc gccttggata ttctgtttat tttgcatgct
     3061 gaccatgaac aaaactgcag caccaatgcc atgcgcggca tcggcagttc gcagcccgat
     3121 ccttattcgt ctgtcgccgg cgcggcggcg gctctctacg gcccgctcca tggcggcgcc
     3181 aatgaggcgg tgttgcgcat gttgatggag atcggttcca aggacaaagt tcctgagttc
     3241 atccagaagg tgaaaggcgg ctcaggccga ttaatgggct tcggccatcg ggtttacaag
     3301 aactacgatc cgcgcgcgcg catcatcaaa aacatcgccg atcaggtgtt cgatgtgatg
     3361 gggaagaatc cgctgctcga aatcgccctc gaactcgaac ggatcgcgct cagcgacgat
     3421 tactttatca aacgcaagct ctatccaaac gtcgactttt attcggggtt gatctaccag
     3481 tcgatgggct tgccgatgga catgttcccg gtgcttttcg cgatcccgag aaccattggc
     3541 tggatcgccc agtgggaaga aatgctcacc gatccggacc agaagctggc ccggcccaaa
     3601 cagatttacc ttggggcggc gagccgcgac ttcgttgaga tgagtaaacg caagtaggat
     3661 ttagcgcaga gtcccgcgca gaccttcaaa gataacccgg agtttctgac ctatttgcag
     3721 ttgtgacgtg gtcaagccgt tgagttccat gagcgcgcta atggcttggc cgtatcgcgc
     3781 cgcgatcgac gcgagagtat cgccccgttt cacttcgtgc gtgaccagct gaaccgattc
     3841 cggttctttg ctgcgctgat attcactggc tttggcaaaa accagagctc tcccgaccgg
     3901 cacgcgcaag cggtaacctc gcgaccccgg aggtgtaatg ttgcgcagca atgccggatt
     3961 gagctcctta atcatcgcga tgtcagtttc ggccatctcc gctgcagcgt ctaatttaac
     4021 caagccctcg gcatcgactt cgtcgaattc gagcggctca tcatagcgca gctgattaaa
     4081 tccgtatttt ttcggattgg tggcgatcat cgcgatggcg acaaatttcg gcacgaagtt
     4141 gcgggtttct tggcttaact gtgctttctc tttgatatcc caaaaatccg aggcgcggct
     4201 ggtttgcagc gccttgttaa ttatccccga gccggcgttg taggccgcgg ttaccaggta
     4261 ccaacggccg tagtactgat gcaaatcttt caaataggcg gccgcagctc tggtggattt
     4321 taccggatcg cgtcgttcgt cgacccaatc gtcgatacgc aggccgtatt gacgcgcggt
     4381 gctggcgacg aattgccata atccgacggc gcctgccggc gatgtggaat tcacctggaa
     4441 ttcgctctcg agcaaggcca aataggccaa ctcttcaggc agaccttcct cgctaaggac
     4501 tttggcgatg atcggcatgt acctaccgga ccgcgccagc agagtctcga aataaacttt
     4561 gttggtgact gaatattggc ggacgaactg gcgcacgcgc gggtggtcga ctactgcttt
     4621 ggagaaatgc agatggcgcc gctctttggg ttgttcgatc gccttatcga tatccttgtc
     4681 taaaaactcc aacagccgat tgtcgttctc ggcgggtctc gctgtgacaa tcgggttggc
     4741 tgcagaacca atggtcggcg ccgctttctc gagtttttcg acgcggtggt ttttcatttc
     4801 caccggaggc gccagaggcg cgggttgagg ccgcggcatc ggcggcacgg cgatggcttc
     4861 gccggcgtcg gcgacgttga tcgctatatc gatttgcggt gttgttggcg cgttactgg
//
LOCUS       DASQKS010000532         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3668517, whole genome shotgun sequence.
ACCESSION   DASQKS010000532 DASQKS010000000
VERSION     DASQKS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668517"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1049)
                     /locus_tag="VE170_13640"
     CDS             complement(15..1049)
                     /locus_tag="VE170_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ16532.1"
                     /translation="MRAPLKSCRALTIALFWVATASGYGAQAAEKTTKPTVKVRISQS
                     AVGARSTILWSAQELGLFAKHGVEAEVVYLRSSPIQMTALSTGEVHFAASGGSPVLGA
                     AAAGQEIKIIASPSNRLSYDLVVRPEIKEAKDLRGKRFGITSLGGTVWLAAYLALEHL
                     GLDATRDQIRINAIGNQTILAQALESAQIDATLLDPFLSRRTKSKGLPVLIELQRAKI
                     PFTNTAWAVSGSFLRERPETVEAVLKAMFEAQAFITAPGNRNSVLQIMARHMKLSDVA
                     LLEDGYQDLLVGLERKPYAALDGLRNIQRIMSTINPKASQAKVEEIIDNRIVRKLDES
                     GFIDALYRKN"
     gene            complement(1057..2091)
                     /locus_tag="VE170_13645"
     CDS             complement(1057..2091)
                     /locus_tag="VE170_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16533.1"
                     /translation="MAGLPTASSLAAQAKPLSPVELALYQGKDREKILLEGARKEGQV
                     VFYTSNTWVAGPVSQEFEKKYPFVKANVWRSDSKALLKRLTEEIAANRNIADVVETSP
                     EYMAIMLREKMLYEHFSPELSAYDDEAKLKGKNGVYGWSNREIYISLGYNSKVIAPAD
                     APKSIKDYLDPKWKGKMSIAGTTTGTQWIGALLELMGREFLEKLSAQEINVQNISGAA
                     LSGLVASGEVPLSPTIFNSNILTHKQKGAPVEWRPLDPVIAGVGISGMVLGSPHPHSA
                     LLFLDYIHSKEGQQVAMKGGLSSPRSDIGSLEQKFKKLYLESKYTPEEYEKKMNEWEG
                     LMRKLFISKR"
     gene            complement(2161..3336)
                     /locus_tag="VE170_13650"
     CDS             complement(2161..3336)
                     /locus_tag="VE170_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ16534.1"
                     /translation="MASATEKYLDGLKSNELLLKFTWDEWLAIAPPYSDGKFTSTEVT
                     ARYQRNGYDWDMHGTLYTPEKETDPHRALVFFHGGAGSEKIMDLTPDGRPGLARVAAA
                     QGFKVLALTYPGHYPPGGVWKVPIPERMPIYLMDRELSQEEIADRNLKCTYNVILQVA
                     GTLTDQHLAGREIIGFGHSTGGPMAAHLTRFSKNTKVIGLVGFGSGGPDGWRKEWRDQ
                     TGAEKYREIPVDHVSRRSPDSFKNSGYVDPEDLTPWGGADDYIRLVSPLRSQMKTSLC
                     DNQHNAAVGILEEYPKRTGLPREEYFDHLEEPHANWLQSIRVLLVAGENDKGHWVNGG
                     APLENKREMFMAQKYAATTRSAHVVFIPRYGHVGYSELYNEKIAYLWLWAHKHGYFD"
     gene            complement(3351..>4050)
                     /locus_tag="VE170_13655"
     CDS             complement(3351..>4050)
                     /locus_tag="VE170_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ16535.1"
                     /translation="YGEYGGKAGIWRIMDALGRNSVKGTIDTNGRIAEDFPDALKELH
                     SGGHEIVGHGWVNDTALSELDAASERALIKRTLDAIASVTGKRPVGWVSPGHRVNDYT
                     FGNFVDEGIFWTGDLPGPDIPQHRMVNGKPLIIMPRLDYANDLSLLFTHKNPARDYFD
                     SFKAAFDCLYTEGQAGAPKLIDCLVHAHIGGRPNIIGVFEECIRYAKGFPDVWFATKG
                     EIAKYYLDTYATKS"
BASE COUNT          795 a         1124 c         1167 g          964 t
ORIGIN      
        1 ctctaaatcg taacctagtt tttgcgataa agagcgtcga tgaagccgga ttcatcgagt
       61 ttgcggacta ttctattatc gataatctct tcgactttgg cctggctggc tttcggattg
      121 atcgtcgaca tgatccgttg aatgttgcgc agcccgtcca aggcggcgta gggtttacgc
      181 tccaggccta ccagcaggtc ctgatagccg tcttccaaaa gcgccacgtc gcttagcttc
      241 atatggcgcg ccatgatttg cagcacgctg tttcggtttc ccggcgcggt gatgaacgct
      301 tgcgcctcga acatcgcttt taataccgct tcgacggtct ccggccgttc tctcaaaaaa
      361 ctgccgctca ccgcccaggc ggtgttggtg aagggaatct tggcgcgctg tagctcgatc
      421 aacaccggca gccctttgga cttggtccgc cggctgagaa acggatcgag caaggtggcg
      481 tcgatctgtg ccgattccaa agcttgggcg agaatcgtct ggttgccgat ggcgttgatg
      541 cgaatctggt cgcgggtggc gtcgagtccg agatgctcca gggcgagata cgcggccagc
      601 catactgtgc cgccgaggct ggtgatgccg aagcgcttgc cgcgcagatc tttggcttcc
      661 ttgatttccg gccggactac tagatcgtag gacagacggt tgctcgggct agcgatgatt
      721 tttatctcct gaccggctgc cgccgcgccg agtaccggtg aaccgccgct ggcggcaaag
      781 tggacttcac ccgtgctgag cgcggtcatc tggatcggac tgctgcgcag atagacgact
      841 tcggcttcga cgccatgctt ggcgaacagg ccgagctcct gggcgctcca gagaatcgtc
      901 gaacgcgcgc ccaccgcgga ctggctgatg cggactttga ccgttggttt tgtagttttc
      961 tcagcggctt gcgcgccata ccccgacgcc gttgccaccc aaaacagggc aatggtcaag
     1021 gcccggcagc tctttagcgg cgcgcgcatg cggcaactag cgcttgctga tgaacagctt
     1081 gcgcatgagt ccctcccact cgttcatttt tttctcatat tcttcgggcg tgtatttgct
     1141 ttcgaggtag agttttttga acttttgctc caacgacccg atatcgcttc gtggcgagct
     1201 gagcccgcct ttcatggcga cctgctgccc ttccttggaa tgaatgtagt cgagaaatag
     1261 cagcgcggaa tggggatgag gcgacccaag caccataccg gaaatgccga cgccggcgat
     1321 caccgggtcc aaggggcgcc attccaccgg cgcgcctttt tgcttgtggg tcaagatgtt
     1381 ggaattaaaa atcgtcggcg ataggggaac ctcgccggac gcgacgagtc cggataaagc
     1441 cgcgcctgaa atattctgca cattgatctc ttgggcgctg agcttctcga gaaattcgcg
     1501 gcccatcagc tcgagcagcg cgccgatcca ttgcgttccg gtcgtggttc cggcaatcga
     1561 catttttcct ttccatttgg gatcgagata atctttgatg ctcttcggcg cgtccgcggg
     1621 cgcgatcacc ttcgagttgt atccgaggct gatgtaaatc tcgcggttgg accagccgta
     1681 aacgccgttt ttgcctttga gctttgcctc atcatcgtaa gcgctcagct ccggcgagaa
     1741 atgctcatag agcatttttt cgcgcaacat aatcgccatg tactcggggc tggtttcgac
     1801 aacgtcggcg atgttgcggt tcgcggcgat ctcctccgtg agccgcttca acagcgcttt
     1861 ggagtcgctg cgccagacgt tggccttgac gaaaggatat ttcttttcga actcctgcga
     1921 gaccgggccg gccacccatg tgtttgacgt gtaaaagacg acctggcctt ctttccgggc
     1981 gccttcgagt aaaatctttt cccggtcctt gccttgatag agcgccagtt caaccggcga
     2041 cagcggtttg gcctgggccg ccaaggagga tgcggttggt aggccggcca ttagcaatac
     2101 aaagatcagc aacgttttag aaagcatcga gcgatctccc ttgtttggct agataataag
     2161 ctaatcgaag taaccgtgct tgtgcgccca cagccatagg taggcaattt tttcattgta
     2221 aagctcggaa tagccaacgt ggccatagcg tgggatgaaa acaacatggg cgctacgcgt
     2281 ggttgcggca tatttttgcg ccatgaacat ctcgcgcttg ttctccaacg gcgcgccgcc
     2341 gttgacccag tgacccttgt cgttttcgcc ggcgacaagc aaaacacgaa tcgattgcag
     2401 ccaattcgca tgcggttcct ctaagtgatc gaaatactct tcgcgcggca ggccggtgcg
     2461 cttggggtat tcctccaaga ttccaacggc tgcgttgtgc tggttatcac acaaactggt
     2521 tttcatttga gaacgcagcg ggctgaccag gcggatgtaa tcgtcggcac cgccccatgg
     2581 agttaaatcc tccggatcga catagccaga gtttttgaac gagtccgggg agcgccgtga
     2641 cacatggtcc actggaatct cgcggtattt ctccgctcca gtctggtcgc gccactcctt
     2701 gcgccagccg tcggggccgc cgctgccaaa tccgaccagg ccgatcactt tggtgttctt
     2761 gctgaaacgc gtcaggtgcg cggccatggg accgccggtg gaatggccga agccgatgat
     2821 ttcacgtccc gccagatgtt gatcagtaag cgtccctgcc acttggagaa tgacgttgta
     2881 ggtgcatttc aaattacggt cggcaatttc ttcctgactt agctctcgat ccatgagata
     2941 gatcggcatg cgctcgggga tgggaacttt ccacacgcct cctggcgggt aatggcctgg
     3001 ataagtgagc gcgaggactt tgaatccctg ggcagccgca acccgcgcca gacccggccg
     3061 tccgtcgggc gtcaagtcca tgatcttttc actgcccgcg ccgccgtgaa aaaagaccaa
     3121 ggcgcggtgc ggatcggttt ccttctccgg cgtgtacagc gtgccatgca tgtcccaatc
     3181 gtaaccgttg cgctgatagc gagcggtgac ctcggtgctg gtaaacttgc cgtcagaata
     3241 gggtggcgcg atggccagcc attcgtccca agtgaacttc aataacagct cgttggattt
     3301 caatccatcc aagtattttt cggtagcgct ggccatggca gtgtctccca ttacgatttg
     3361 gtggcgtagg tgtcgagata atacttggcg atctcgcctt tcgtggcgaa ccaaacatcg
     3421 ggaaaccctt tggcgtagcg aatgcattct tcgaagacgc cgataatgtt gggccggccg
     3481 ccgatatggg catggaccag gcaatcgatc agcttgggag cgccggcttg gccttcggtg
     3541 tagagacaat cgaaggcggc tttgaaacta tcgaaatagt ctcgcgcagg gtttttatgg
     3601 gtgaacaaga ggctcaaatc attggcgtaa tcgagccgtg gcatgatgat caaaggtttg
     3661 ccgttgacca tgcgatgctg cggaatatcg ggcccgggca ggtcgccggt ccaaaaaatg
     3721 ccttcgtcga cgaaattgcc gaacgtataa tcattgacgc gatggccggg gcttacccaa
     3781 cccacgggac gtttaccggt taccgatgcg atggcgtcta aggtacgttt gattaaagcc
     3841 cgttcactag cggcgtcgag ctccgaaagg gccgtatcgt tgacccaacc gtgtccgacg
     3901 atttcatggc cgccactgtg tagttctttt aacgcatcag gaaaatcttc ggcgatgcgg
     3961 ccgttggtgt cgatggtgcc tttgacactg ttgcggccga gcgcatccat aatgcgccag
     4021 atgccggcct tgccgccgta ttcgccgtag
//
LOCUS       DASQKS010000533         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4898213, whole genome shotgun sequence.
ACCESSION   DASQKS010000533 DASQKS010000000
VERSION     DASQKS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898213"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VE170_13660"
     CDS             <1..343
                     /locus_tag="VE170_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ16536.1"
                     /translation="TNKRSITLDLESADGRTIFLQLAGKADVVIETYSPAQSDRLGLN
                     YAQLSVVNPRLVVASITAFGRSGPWRDYKADDIAAIALGNLLSSVARPACAATAIAAR
                     TLDCGTGNPTS"
     gene            280..1371
                     /locus_tag="VE170_13665"
     CDS             280..1371
                     /locus_tag="VE170_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ16537.1"
                     /translation="MRGDGHRGEDPRLWNRKPDFVKLQRGKKSFTVNMQTAKGRDLAK
                     RLVQKSDVLIENFGLGILEKWGLDYPQLQELNPACILIRVKGMGCTGPHATDLTYGPN
                     VINNMGATYLWNYPDPMTATAEPRTQHPDFMGGVAGAFGVVAALLQRTVTGRGDMIDN
                     AQLEIGASLLGPKYMEYSVNRRDPKPQGNRSLIAAFYGTYPCQGEDRWCVISVYTQAD
                     WESFTGVVLQQSGAKPAAKFATHLGRVRHREELDQWVSQWTVQQDPYAIMEILQSVGV
                     CAAVVQEVEDQFKRDRQYAATGFLVEMTEPEAGNIATENVPIRLSETPGEVRGCAPLM
                     GQHTYEIARDLLGLADHEIKQLEAEQVLY"
     gene            1673..3913
                     /locus_tag="VE170_13670"
     CDS             1673..3913
                     /locus_tag="VE170_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYJ16538.1"
                     /translation="MLHGAKALALLGGVGLAMLLVYGVVLIPFTPSIADLRRAKIDQP
                     SVLISADGRRLATLKPMNREWVRLSQISPHVITALIATEDHRFYSHYGVDLLRVVSGI
                     LRTFIGDPQGGSTLTQQLARNLYPEGIGRQRTVTRKLKETITAIKIEFAYSKTEILET
                     YLNTMPFLYNAFGIEMAARTYFDKPARKLDVAESATLIAMLKGTAYYNPVLNPERSLK
                     RRNVVLAQMVKRGVLSEAEFNRQKLRPIRLDFERQPEAVGSIPHLAMQIRKWLIGWAD
                     RNDKNIYTDGLIVHTTIDSRLQALANQAVNRQMETLQAVADVEWGMSSKQLLSTEVNT
                     YVQQRRRVQPFGYFWKTQSALFDSFIRESPAYRRALEAGTKPEAALAQLKNDKEFIGK
                     LRAEKSRLQAGFVAIDPANGHVKAWVGSRDFRTDQFDHVVQARRQPGSTFKPFVYGAA
                     IEQGMSPDKHFVDRAVEISLPGGKVWRPADATAPTGRSMTAREGLIYSKNTITAQVMQ
                     EIGPRKTAELARLMGITQSKLEEVPALALGTSPVTLLEMVSAYSTLAAGGQYRPPIFV
                     SRITDKNGNELVRFVSETQRVLSDKSAGELINILRDAIDQGTGQPVRAQFAVRADVAG
                     KTGTTQDNTDGWFILMHARLVAGSWVGFNDSRITMRSDYWGQGAHNALLVVGDFFQHS
                     FNSQFVAAASRFPYERPYQSVWDPVLDTAKDWFGGILRDWGFGDRTTPAPAPQRRERD
                     LDSTRE"
     gene            4009..4449
                     /locus_tag="VE170_13675"
     CDS             4009..4449
                     /locus_tag="VE170_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYJ16539.1"
                     /translation="MELVRFEPFARVGNFRSLISDLFDENLERSPARPSVSQWHPAVD
                     VLESKDAYLIRAELPGMNRDDIKVEVKDGKLVLTGETKSEKPAEGTTYRHVERVAAKF
                     WRSFSLPETAKADGVEATYKDGILEIRVPKAEESKPRQIEINVQ"
BASE COUNT         1058 a         1377 c         1288 g          919 t
ORIGIN      
        1 caccaacaaa cgcagcatca cgctcgatct ggagagcgcc gacggccgga cgatttttct
       61 ccaactcgcg ggcaaagccg acgttgtcat cgaaacctat tcacccgcgc aatcggaccg
      121 gctcggtttg aactatgcgc agttaagcgt tgtcaatccc aggcttgtcg tcgcctccat
      181 caccgcgttt ggccgatcgg gtccatggcg cgattacaaa gccgatgata tcgccgccat
      241 cgccctcggc aatttgttgt catccgttgc gagacccgct tgcgcggcga cggccatcgc
      301 ggcgaggacc ctagactgtg gaaccggaaa cccgacttcg tgaaactcca gcgcggcaag
      361 aaatccttta cggtgaatat gcaaaccgcc aaagggcgcg acctggctaa gcgattagtg
      421 caaaagtccg atgtcctcat cgaaaatttc ggcctcggca tcttggaaaa gtgggggctc
      481 gattatcctc agcttcagga actcaaccca gcgtgcattc tgattcgcgt caaaggcatg
      541 ggctgcaccg gcccccacgc caccgacctc acctatggcc cgaatgtgat caacaacatg
      601 ggcgctacct atttgtggaa ctatcccgac ccgatgacgg cgaccgcaga gccaagaacc
      661 cagcacccgg atttcatggg cggagtcgcc ggcgccttcg gagtcgttgc cgccttactg
      721 cagcgcacgg tgaccggccg cggcgacatg atcgataacg cgcagctgga aattggcgct
      781 tcgcttctcg gacccaaata tatggaatac tcggtaaacc ggcgcgaccc caaaccgcag
      841 gggaatcgca gcttgatcgc cgcgttctac ggaacctacc cttgccaagg tgaagaccgc
      901 tggtgcgtaa tctcggtcta cacccaagct gactgggaga gttttaccgg tgtggtgctg
      961 caacaatctg gagccaagcc cgccgccaag tttgcgactc acctagggcg ggtgcggcac
     1021 cgggaggaat tggaccagtg ggtcagtcaa tggaccgtgc aacaggaccc ttacgcgatc
     1081 atggaaattt tacaaagcgt cggggtgtgc gccgccgtcg ttcaagaagt ggaagatcaa
     1141 ttcaaacgcg accggcaata tgccgcgacc ggattcctgg tcgagatgac cgaacccgaa
     1201 gccggcaata tcgcaaccga gaatgttcct atacgtcttt ccgagacgcc cggtgaggtg
     1261 cgcggctgcg cgccgttaat ggggcaacat acctacgaaa tagccagaga tttattgggt
     1321 ctggcagacc acgagatcaa gcagctcgag gccgaacaag ttttgtacta gacatccgca
     1381 cggctctcgg agcgatgatt catcaagcaa tcgcaatgct ggacgttacc agcgactcgc
     1441 ccctttagtt cttattttca tcacatacaa taagtacggt gacattaatt tgagtcaccg
     1501 cccattcgtc taagcagatt gcccgctgga actgcactgc gatggcccgg gaacaagaga
     1561 attgatcgtg gctgaaaact cgcctcctac cggacgacta agacgctttt accagcggct
     1621 gccggccggc gtccgggcgg ccctcgatcc taacttgagc actcgggtca gactgcttca
     1681 tggggcgaag gcgctggcgc tattgggcgg tgtcggactg gccatgttgt tggtttacgg
     1741 cgtagttttg ataccgttca cgccgagtat cgccgatcta cgacgagcca agatcgacca
     1801 acccagtgtg ctgatttccg ccgacggccg gcgtcttgcg acgcttaaac cgatgaaccg
     1861 cgagtgggtt cgtttaagtc aaatctcgcc gcacgtgatc acggccctga tcgccaccga
     1921 agaccaccgc ttctatagcc attacggcgt cgatttactg cgcgtcgtaa gcggcatcct
     1981 gcgcacattt atcggcgatc cccagggcgg ctccacgctc acccaacaac tcgcgcgcaa
     2041 tttgtatccg gagggaatcg gccgtcaacg cacggtcaca agaaaactga aagagacgat
     2101 caccgcgatc aagatcgagt tcgcctatag caaaacagaa atcctcgaaa cctatctcaa
     2161 taccatgcct tttctataca acgccttcgg catcgaaatg gccgcgcgca cctacttcga
     2221 caagccggcg cgtaaactcg acgtcgccga gagcgcgaca ctgatcgcca tgttaaaagg
     2281 caccgcctat tacaatccgg tgctcaatcc ggaacgctcg ctcaagcgcc gcaacgtcgt
     2341 gttggcgcaa atggtcaagc gcggcgtgtt gagcgaggcc gaattcaacc ggcaaaaact
     2401 ccgccccata cgcctggatt tcgaacggca gccggaagcc gtgggctcca tcccgcatct
     2461 tgccatgcag attcgcaaat ggttgatcgg ttgggcggac cgcaacgaca agaatattta
     2521 taccgatggc ttgatcgtcc acaccaccat cgactcgcgt ttgcaggcgc ttgccaacca
     2581 agcggtaaat cgccagatgg agaccttgca agccgtcgcc gacgttgagt ggggcatgag
     2641 ttcgaagcaa ttgttatcga cggaagtcaa cacctacgtg cagcaaaggc ggcgcgtgca
     2701 gccattcggt tatttttgga aaactcaatc cgccttgttc gattccttta tccgggaaag
     2761 cccggcttat cgccgcgcgc tggaggccgg gaccaagcct gaagctgcat tggcgcagtt
     2821 gaaaaacgac aaggaattca tcggcaagct gcgcgccgag aagagccgtt tacaggcggg
     2881 attcgtcgcc atcgatccgg ccaacggtca cgtcaaggca tgggtcggca gccgtgactt
     2941 ccggactgat caattcgatc acgtcgtgca agccagacgg caaccgggct cgaccttcaa
     3001 accgttcgtt tatggcgccg cgatcgagca gggaatgtcg cccgataagc acttcgtcga
     3061 ccgcgccgtg gaaatttccc tgcccggcgg caaagtatgg cgtcctgccg atgccacagc
     3121 gccgaccggg cgatcgatga ccgcgcgcga aggtctgatc tactcgaaga acaccatcac
     3181 cgcccaggtc atgcaggaaa tcggcccgag aaaaaccgcc gaactggcgc gattgatggg
     3241 gatcactcag agtaaactcg aggaggtgcc ggcgttggcc ctcggcacca gtccggttac
     3301 cctgctggaa atggtttccg catacagcac cctcgccgcc ggcggccaat atcgaccgcc
     3361 gatattcgtc agtcgcatta ccgataagaa cggcaatgag ctggtccgct ttgttagcga
     3421 aacccagcgc gtgctgtccg acaagagcgc cggcgaactg atcaacatac tgcgcgacgc
     3481 catcgaccag ggaaccgggc aaccggtgcg cgcccagttc gctgtgcgcg ccgacgttgc
     3541 gggaaaaacc ggcaccaccc aggacaatac cgacggctgg tttatcttga tgcatgcgcg
     3601 gctcgtcgcc ggctcatggg tcggcttcaa cgattcgcgc atcaccatgc gcagtgacta
     3661 ctggggtcag ggtgcgcata acgccttgct cgtggtgggc gatttctttc agcacagctt
     3721 caacagccaa tttgtcgccg ccgcctcgcg ctttccctat gaaagacctt atcaatcggt
     3781 ctgggatccg gtgctcgaca ccgcgaaaga ttggtttggc ggcatccttc gagattgggg
     3841 attcggcgat cgaaccacac cggcgccagc gccgcagcgc cgtgaacggg acctcgattc
     3901 gacgcgggaa taaatcgcgc ccataagcac gacaccggct tttgtcaagc cacttgacgc
     3961 agttataccc ggtgcttatt ttctcgtcat cacaaggagg ttacgaatat ggaactcgta
     4021 agatttgaac cgtttgcccg cgttggaaat tttcgctctt taatttccga tttattcgat
     4081 gagaatttag agcggtcgcc agcacggccc agtgttagtc agtggcatcc agcagtggat
     4141 gttttggaga gcaaggacgc gtatttgatt cgcgcggaac tgcctggcat gaaccgggac
     4201 gacatcaagg tcgaggttaa agacggcaag ctagtgctca ccggcgaaac caagtcggag
     4261 aagccggccg agggaaccac ataccgtcac gtcgaacggg tcgcggcaaa gttctggcgc
     4321 tcgttttcac tgcccgagac cgcgaaagcg gatggcgtcg aagctaccta caaagacggc
     4381 attctcgaga tccgcgtgcc aaaagcggag gagagcaaac cgcggcagat cgaaattaac
     4441 gtgcagtaat tcgctgctaa aaccgccaat gcgacaaggg aggcaatacg cctcccttgt
     4501 cgttacggcg cgattcactg cggccccctt tagcttcgaa ccggcccgca ccctgccatt
     4561 gtagtctatg taatccgcac ttttgctcga tgcgcgatcc ctgagcgagc aatcgctgac
     4621 tgcgacaaat tgttctgtaa tt
//
LOCUS       DASQKS010000534         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4079807, whole genome shotgun sequence.
ACCESSION   DASQKS010000534 DASQKS010000000
VERSION     DASQKS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4079807"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VE170_13680"
     CDS             complement(<1..699)
                     /locus_tag="VE170_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16540.1"
                     /translation="MNETFDLLLTGGTVLNPATKTHEKLDVGVKGDRVTAIRAEIPPT
                     GVERVIDATGCYVTPGLIDFHVHSYWGVNPYGCDLDAVCLATGVTTALDAGSAGPVNL
                     LGFRKLVYERSKTRMIAFVALAQHGVLNEPGELLNLGFADLDGSAQAVIDNRDLGVGI
                     KVRLHKKSIGDNSRDALRLAIKAGEASGTPMMVHVGATAIGMDEIADTLRPGDIITHC
                     YTPQKPSIIDDKGKL"
     gene            complement(944..1981)
                     /locus_tag="VE170_13685"
     CDS             complement(944..1981)
                     /locus_tag="VE170_13685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16541.1"
                     /translation="MLSIDADAHVIECEKTWDYLEGPDKRYRPVPIAVDMPTGKKSNF
                     WIIGGRLIGGRDNVGQDTSKESREMANIDSRIKHMDDIGTGVQVLYPTLFLRPVTDNP
                     RIELALCKSYNRWMADIWSKDKKRLRWAVQLPLLTMDAALDELRWSKGNGACAVFMRG
                     IETNHVLHDPYFFPLYEEASRLNLPIGIHSGIGNFTVNDMLGAEPFRVAKLIVIGGFH
                     SMILNGILEKFPKLRVGAIEVAAQWVPYIVHDLNLRFPKLEGKEARPDVLTGSSQLFV
                     ACQTDDDLPYVLKYAGENCLMIGSDYGHADNASELLALAKLKETSDIDPKVIDKILTH
                     NPAKFYGLDVD"
     gene            complement(2346..3365)
                     /locus_tag="VE170_13690"
     CDS             complement(2346..3365)
                     /locus_tag="VE170_13690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16542.1"
                     /translation="MKVIDADAHVIENESTWDYMLESEKAFKPKIVGASNGKDPFDYW
                     MIDGRVIPRNNVGRDLPVESREMSDLSARIKHMDQLGIDFQIIYPTFFITPVSRRPDI
                     DLAVSRSYNRWMADIVRKQPDRFRWVLVPPLQSQEHLAEELRFGKENGACGVYMRGLE
                     CERSLSDPYFYSLYEQASALDLPICVHSANGAFTTYDFFADDPGFSKFKLAVIGAFHS
                     LVFHAIPQRFPKLKIGIIEVSAQWIPYALHDLSRRFARRGRAFPQNILKENRIYVTCQ
                     TDDDLDQVLRYAGDDNLVIGTDYGHADNAAEIEALRKLRTDGKISGRVIDKILQDNPS
                     AFYGL"
     gene            complement(3593..>3820)
                     /locus_tag="VE170_13695"
     CDS             complement(3593..>3820)
                     /locus_tag="VE170_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16543.1"
                     /translation="YVAEEVRDANICVVTFSPRVPIATETAPEEALKRLPGPDVLGTG
                     FVLCAQLPMAQSGKCFAYENGKLVDELAREG"
BASE COUNT          812 a         1131 c         1026 g          851 t
ORIGIN      
        1 cagcttgcct ttgtcatcga tgatcgacgg cttctgcggc gtgtagcagt gggttatgat
       61 gtcgcccggc cgcaaggtat cggcgatctc gtccatgccg atcgccgtgg cgccgacatg
      121 gaccatcatc ggcgtacccg acgcttcgcc ggccttgatt gccaggcgca aggcgtcgcg
      181 gctgttgtcg ccgatggatt tcttgtgcaa gcggactttg atgccgacgc cgagatcgcg
      241 gttatcgatc accgcctgcg ccgagccgtc taagtcggcg aagcccaaat tgagcaactc
      301 gcccggttcg ttgagcacgc cgtgctgggc taaggcgacg aatgcgatca tgcgtgtctt
      361 ggatctttcg tagacgagct tgcgaaagcc gagcagattc accggccccg ccgatccggc
      421 atcgagcgcc gtcgtcacgc cggtggccaa gcagacggcg tcgagatcgc agccataggg
      481 gttgacgccc caatagctgt gcacatgaaa gtcgatcaac cccggcgtca cataacatcc
      541 ggtggcatcg atgacacgct cgacaccggt aggtggaatc tccgcccgga tcgcggtgac
      601 ccgatcgcct ttgacgccga cgtctaactt ctcatgggtc ttggtcgctg gattgagcac
      661 cgtgccgccg gtgagcaaca agtcaaacgt ctcattcatt ttaggtctct cgtagtattg
      721 tcctcacttc gggagtttac cgcttctccc tttgggagaa ggctgggggc agggcgaagt
      781 tcgctcgacc cgttaaagac cctcaccctc gccctctccc acgaaacgtg ggcgagggta
      841 taattcacag aaaccagagc taccccaccc atgtcatttc gatcgaaggg agaaatctct
      901 ttccgtttcc cgacgaacac gaaacatgaa cacgatagcg ttatcaatcc acatccagcc
      961 cataaaactt tgccggatta tgggtcagaa ttttatcgat cactttggga tcgatgtcgc
     1021 tggtttcttt caacttcgcc agggccaaca gctcgctggc attatcggca tggccgtagt
     1081 cagagccgat catcaggcaa ttctcgccgg cgtacttgag cacgtagggc agatcgtcgt
     1141 cggtctggca ggcgacgaac aactggctgc tgccggtcaa gacgtcgggc cgcgcttctt
     1201 tgccttcgag cttgggaaaa cgcagattca aatcgtgcac gatatacggc acccactgcg
     1261 ccgcaacttc gatggcgccg acgcgcagct tgggaaactt ttccaggatg ccgttcaaga
     1321 tcatcgaatg aaatccgccg atgacgatca gcttggcgac gcgaaacggc tcggcgccga
     1381 gcatgtcgtt gacggtgaaa ttgccgatac cggaatgaat gccgatcggc agattgagcc
     1441 ggctcgcctc ttcgtagagc gggaaaaagt acggatcgtg cagcacgtga ttggtctcga
     1501 tgccgcgcat gaacaccgcg caggcgccgt tgcccttgga ccagcgcaat tcatccaacg
     1561 ccgcgtccat ggtcaaaagc ggcaactgca ccgcccagcg caaacgtttt ttatccttcg
     1621 accaaatgtc cgccatccag cggttgtagg atttgcacag ggccagctcg atgcgcggat
     1681 tgtccgtcac cggccgcaga aacaacgtcg gatagagcac ctgcacgccg gtgccgatgt
     1741 cgtccatatg cttgatccgc gagtcgatgt tggccatctc gcgggattcc ttcgaggtat
     1801 cctggccgac gttgtcgcgc ccgccgatca agcgcccgcc gatgatccaa aagttgctct
     1861 tcttgccggt cggcatgtcg acggcgatcg gcaccggccg atagcgttta tccgggcctt
     1921 ctaaataatc ccaagtcttt tcgcactcga tcacatgcgc gtcagcatcg atacttaaca
     1981 tctgaaacct ccattagggt aatctgcctt ataaacgtct gatttcacca cgaagtgaat
     2041 catgatctta cgctcgtcta cagtatatga aaatgcatgg gattcgctcc ataggcagtc
     2101 tacttgacgg gtcattcccg cgcacgcggg aatctaggtt tgatctgcgc ccaattagcc
     2161 tggatacccg ctttcgcggg tatgacggaa cccagattcc ctcttcattt tggcgaccat
     2221 tcccttacat gtattttcta aggaggcacg aaggacacaa aattccggaa aaataatatt
     2281 tcgaacttcg tcctcttctg tccttcgtgg tgaatacttg ccccaaaaaa ccgcagccat
     2341 tcgccttaca aaccgtaaaa tgccgatgga ttatcctgca agatcttgtc gatcacccgc
     2401 ccgctgattt taccgtcggt gcgcaacttg cgtagcgctt cgatctcggc ggcattgtcg
     2461 gcatggccgt agtccgtgcc gattaccagg ttatcgtcgc cagcgtaacg cagcacctgg
     2521 tcgagatcat cgtcggtctg acaagttaca tagatacgat tctccttcag gatattctgg
     2581 gggaaggccc ggccgcgccg ggcgaagcgc cgcgacagat cgtgcagggc gtagggaatc
     2641 cactgtgcgc tgacttcgat gatgccgatc ttcaacttcg ggaagcgctg cgggatggcg
     2701 tgaaacacca gtgaatgaaa cgcgccgatg accgccagct tgaatttcga gaagcccgga
     2761 tcgtcggcga aaaaatcgta agtggtaaaa gcgccgttgg ctgagtgcac gcagatcggc
     2821 agatccagcg cgcttgcctg ttcgtacaac gaatagaagt aaggatcgct gagactgcgc
     2881 tcgcactcca ggccgcgcat atagacgccg caggcgccgt tctccttgcc gaatctcaat
     2941 tcttccgcca gatgttcctg gctttgcagc ggcggcacca gcacccaacg aaaacgatcc
     3001 ggctgctttc taacgatgtc ggccatccag cggttgtagc tgcgcgaaac ggcgagatcg
     3061 atgtccgggc gccggctcac cggcgtgatg aaaaacgtcg ggtagataat ctggaagtcg
     3121 atgcccagct ggtccatatg cttgattcgc gcgcttagat cgctcatctc gcgcgactcc
     3181 accggcagat cgcggccgac gttattgcgc ggaatcaccc ggccgtcgat catccaatag
     3241 tcaaatggat ctttgccatt gctcgcgccg acgatcttcg gcttgaacgc cttctccgac
     3301 tccagcatgt aatcccaagt cgactcgttc tcgatgacat gggcgtccgc atcgatgact
     3361 ttcatcgttg actccctact gttctgactc cgaagttcgt tgaataggtg gcagtgttca
     3421 ttagtgtagt tcttctttga aaaaactcat cgttacccat aagtttccgc tgaacacttc
     3481 ggctgcaaga gacatcgata atctgacttc aaccgctcaa atcctggcca gctatgcgtt
     3541 accccccttt ggaaaagagg agttagggga gattttttgc ggccaggcga cttcaccctt
     3601 cccgcgccag ctcatccacc agtttaccgt tctcatacgc gaaacactta ccgctctgcg
     3661 ccatcggcaa ctgcgcacag agcacgaatc ccgtgcctaa aacatccgga ccgggcagac
     3721 gcttgagcgc ctcttccggc gcggtctcgg tggcgatcgg cacgcgcggc gagaacgtca
     3781 cgacgcaaat gttcgcgtcg cgcacttcct cggcgacgta
//
LOCUS       DASQKS010000535         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4899969, whole genome shotgun sequence.
ACCESSION   DASQKS010000535 DASQKS010000000
VERSION     DASQKS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4899969"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1332)
                     /locus_tag="VE170_13700"
     CDS             complement(181..1332)
                     /locus_tag="VE170_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16544.1"
                     /translation="MARDGYKILDSDMHVFEPYDLYEKYMDAKWGERIPRGKPRTKHG
                     QIKFTYSDGRLLRPSGTQALAATAPNPNSGESAPGEAQVAHRYAKPLARDYDAVSQLA
                     AMDDEGLDVAVLFRTFPLHCDDSLDPEYANALCRAWNDWMADFCRVNPERLRPSALIT
                     LHDVDLAVDETRRAIDQLGAICLCLVPEPSNGKHIHDRYYDPVWREAEKLGVPVCFHP
                     AASPNQDQAVHRFRGHANEAVLINAFRNPVELMFAVGSFCAGGVLERFPNLRVAFLEG
                     NCGWLPWALYRLDERWAMRKEYCNETMSMKPSDYFLRQCFISVDVEEDLLGDVIRRIG
                     DDNIVLSTDYPHADSHWPDAVNHFMAMEMPSGARRKILWDNCARLYGVS"
     gene            1568..3097
                     /locus_tag="VE170_13705"
     CDS             1568..3097
                     /locus_tag="VE170_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16545.1"
                     /translation="MLAANAKLTLIIFLAAAAILGNSSFADSQTRITVGVTETIDTHN
                     PYGDSVSLMYGIWSEITGPFCTYSYAKGDFEGRLAEKWKVENPTTWIFSLNKNYKFND
                     GSPVSADDVVHSIVNRVIKDPQSKQKASVAPSIVNAEVIDKLTVKITTDKPTAPLLSF
                     LCDRLIVTSKAMFDKYGRDVADKEHMIGAGPYRLKELIPGQRLVMAKNPNHPEAKRNP
                     RAPDEVIYRIMREPEQRVTALLNDEIQIGQFVPPHMRQRVETNKSLRIMPVDSVEIMF
                     LAMQPKPPFDKKEVRQAVCQAINRDQIVATLLESFASRLDGPLGPGQYGYDPTLKPKL
                     NYDPEKAKKLLAQAGFPNGIDAELQTPVGRYTLDKQLTEAMIPMLNAVGIRAKLATPE
                     WATLWANVQKGTVPFYYMGRGSVQDPSAALHQYFHTGETPRIGYSNPKVDELLDKEQQ
                     EFDPKKRRQYLSQAMSLITEDAPACFMWRHKLLWGMHSRVDYKPLPDSRVYAIEMAVK
                     K"
BASE COUNT          753 a          997 c          881 g          665 t
ORIGIN      
        1 ggaaccctgt taccctcttc atattggcga ccatcccctt acatatattt tcgaaggagg
       61 cacgaaggac accaaggttt cggagaattg aaacgctgaa cttcgtgctt ttcatatcct
      121 tcgtggtgag tcttcccctc cgcgttttct ccctttgcgt tatttgcggc caatttattc
      181 ttaactcacg ccatacagcc gcgcgcagtt gtcccacaga atcttcctgc gcgcgccgct
      241 cggcatttcc atcgccatga agtggttgac agcgtcgggc cagtgcgagt ctgcgtgggg
      301 ataatcggtc gagagcacga tgttatcatc gccgatccgc ctaatcacat cgccgagcag
      361 atcctcttcg acatcgacgg agatgaaaca ctggcgcagg aaatagtcgc tcggcttcat
      421 gctcatggtt tcattgcagt actccttgcg catcgcccag cgctcgtcca aacggtacag
      481 cgcccagggc agccaaccgc agttgccctc gagaaacgcc acccggagat tgggaaatct
      541 ttcgagcacg ccgccggcgc agaagctgcc gacggcgaac atgagttcga cgggattgcg
      601 aaacgcattg atcagcaccg cctcgttggc gtggccgcga aaacggtgca ccgcttgatc
      661 ctgattgggg cttgccgctg gatggaaaca gaccggcacg ccgagctttt ccgcttcgcg
      721 ccacaccggg tcgtaataac ggtcatggat atgtttgcca ttgctcggct cgggtacgag
      781 acagagacaa atggcaccca actggtcaat cgcccgccgg gtctcgtcca ccgccagatc
      841 gacatcgtgc agggtgatca gcgccgatgg ccgcagccgt tcaggattca ccctgcaaaa
      901 atccgccatc cagtcattcc atgcacggca cagcgcgttg gcgtattcag gatccaaact
      961 atcgtcgcag tgaagcggaa aagtcctgaa caacaccgcg acgtccaagc cctcgtcgtc
     1021 catcgctgcc agctgcgata cggcatcgta atcgcgcgcc agaggcttgg cataacgatg
     1081 cgcgacctgg gcttctcccg gcgcgctttc gccgctgttg ggattgggcg cggtcgcggc
     1141 taacgcttgg gtgcccgaag gacgcagcag ccggccgtca gagtaagtaa attttatttg
     1201 cccatgcttg gtgcgcggct tgccccgggg gatgcgctca ccccatttgg cgtccatgta
     1261 cttctcgtaa agatcgtagg gttcgaaaac atgcatatcg ctgtcgagga tcttgtaacc
     1321 gtcgcgcgcc atgggtgaca tccgcatttc taatcgccga agactctctt catcctggca
     1381 acaaggactg gataacaacg agtcgtaact tggcccatga ctagtctcag ccccgcgcca
     1441 tgtcaactaa aaaagtcccg gcgcgccgag cgcaacctgg ggccaaagtt catcttgaca
     1501 acaccgcggg gagctgtaaa agattccgca gcgcattgac gtcacgaata aatttcggga
     1561 ggttttcatg ctcgcagcga acgcaaaact cacgttgatt atttttttgg cagctgccgc
     1621 aatcctgggc aattcaagtt tcgccgactc ccaaacccgc atcaccgtcg gcgtcaccga
     1681 aaccatcgat actcacaacc cctacggcga cagcgtttca ctcatgtacg gcatctggag
     1741 cgaaatcacc ggcccgtttt gcacttatag ctacgccaag ggcgattttg aagggcggct
     1801 tgccgagaag tggaaagtgg aaaatcccac cacctggata ttctcgttga acaagaacta
     1861 caaattcaac gacggttctc cggtgagcgc cgacgacgtc gtccattcca tcgtcaaccg
     1921 ggtgatcaag gacccgcagt ccaagcagaa agcgtccgtc gcgccgtcga tcgtcaatgc
     1981 cgaggttatc gacaagctca ccgtcaagat caccaccgac aaaccgacag cgccgctgtt
     2041 gtcgtttctc tgcgaccgct tgatcgttac cagtaaagcg atgttcgaca aatacggccg
     2101 cgatgtcgcc gacaaagagc acatgatcgg cgccggcccc tatcgcttga aagaattgat
     2161 ccctggacag cgtctggtca tggcgaaaaa ccccaatcat cccgaggcaa aacgaaatcc
     2221 ccgcgcgccg gacgaagtga tctatcggat catgcgcgaa ccggagcaac gcgtcaccgc
     2281 tttgttgaac gatgagattc agatcggcca gttcgtgccg ccgcatatgc gccagcgggt
     2341 cgagaccaat aagagtctgc gcatcatgcc ggtggattcc gtcgagatca tgtttctggc
     2401 gatgcaaccc aaacctccct ttgacaagaa ggaggtccgc caggcggtgt gccaggccat
     2461 caaccgcgac cagatcgtcg caactttact ggagagtttc gccagccgcc tcgacggccc
     2521 gctcgggccg ggacagtatg gttacgatcc aactttgaag cccaaactca actacgatcc
     2581 ggaaaaagcc aagaagcttt tagcgcaggc cggttttccg aacggcatcg atgcggagct
     2641 gcaaacccca gtggggcgct acaccctgga caagcaactc accgaagcga tgatcccgat
     2701 gctcaacgcc gtcggcattc gcgccaagtt ggcgactccg gagtgggcga cgctctgggc
     2761 caacgtgcaa aaaggcaccg tgcctttcta ttatatgggc cgcggttcgg tccaggaccc
     2821 gagcgccgcg ctgcaccagt attttcacac cggcgagaca ccccgcatcg gctattcaaa
     2881 tcccaaggtc gacgaacttc tcgacaagga acaacaggaa ttcgatccga aaaaacgccg
     2941 ccaatacttg tcccaagcga tgagcctgat caccgaggac gcgccggcct gcttcatgtg
     3001 gcggcataaa ttgctatggg gcatgcacag ccgcgtcgat tacaagccgc tgccggactc
     3061 acgcgtctat gccatcgaga tggcggtaaa aaaatagatc aggccgaacc gtaatacggc
     3121 gaatatttgt cgcggagtaa agtgtcatgc agaaggaaaa atttgtcgac gttgacggca
     3181 tccgcacccg ctatttcgaa gccggcagcg gccccgacct ggtgttgttt cacggcggcc
     3241 acttcggttc ccacgatgcc gccgactgcg ccgaggattg gagcctcaat ttcgac
//
LOCUS       DASQKS010000536         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2829403, whole genome shotgun sequence.
ACCESSION   DASQKS010000536 DASQKS010000000
VERSION     DASQKS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829403"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..606)
                     /locus_tag="VE170_13710"
     CDS             complement(115..606)
                     /locus_tag="VE170_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ16546.1"
                     /translation="METQSSFNLTVTVNGQAWSGEVAIEETLLEFLRQRLHLTGTKRS
                     CESEVCGACTVLVDNRAVSACNYLAFEANEKSVRTIEGVAHGDKLDPLQEAFIRNIGA
                     QCGYCTPGQIMAAKALLMENPNPSREQIAEWLSGNICRCGAYGAIAQSILEAAAAYQS
                     KEE"
     gene            complement(615..1484)
                     /locus_tag="VE170_13715"
     CDS             complement(615..1484)
                     /locus_tag="VE170_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYJ16547.1"
                     /translation="MLNPLKLVQPTSVREASQALAEFGDKAKLYAGGAELLLLLRNGL
                     IDAEILIDVKKIERLRRLDLRNGTLHVGACLTHHELATNAAIRERAPSFAYAESQVAN
                     IRVRNQGSLGGNLAFNDPHSDPGTVLLVHNASVTIGNAKGERRLALDDFFVDMYATAL
                     EAGELLLEVEIPSLPAGMTSAYQRLHRYQRPTLGVAVALRIDNEKVAEARLAVGCIGP
                     KAQRLTALEEKVNGVPLTDTKRIFADQKKYLRDVLRPVDDLLGSVEYKLYMTGVMLGE
                     ALAQAAGERIPED"
     gene            complement(1525..>3739)
                     /locus_tag="VE170_13720"
     CDS             complement(1525..>3739)
                     /locus_tag="VE170_13720"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ16548.1"
                     /translation="SGRAEYIEDLPDLPNTAYGATLLSPYSHAKIRRIDCTEALKIPG
                     VLGVVDREHLDGVNPRLKLAPHEHLKITDDQDFVAINKVRYDGDLVALVVAEDRRTAE
                     RALAKIQVDYDPLPPVFDAVAAMEPGAPLLHEDRGGNLLVEDGLVWGDVDEGFKQAHR
                     IFEETYSSPSMFHHPMEPVGGFLAHYVNGEANIWLPTSSPTRDAAEFAHFLGIEPDKV
                     RVRVPYVGGGFGSKIPTPAHFAALFLSRKIGRPIRLIPSAEESFRQNARHAEVFKARV
                     GVKTDGTLTALDVDLVVDTGAYTTGGATAVHNSVISAWGCYRIPHLRIRGKCVYTNKV
                     PAGHTRATGKIQTTWGIECAMDSVARQLGIDPYEFRKKNVLLRGDFVCKGTPNMDTDY
                     LDLMSEAIAGIAWDGQANPAPSLNGPRWARGRGMALSLRHGSQGGGRAYALVTLDARG
                     VLTIQHNAPEIGQGTHNLFSVVASQTLNLPQSQIHVGTPDTAVNLPFAGVSAQRTTMQ
                     MGNAVHNACKTLQEDLLGLAAQVKGGEAKDWQIVDGWLCWGESSFSINEIIRAVGASV
                     VLKSVGYHSVPPMVKDSAFAGMDHWAPSGAAVDLEVNRDTGELRILGYAVIADAGKTI
                     HYSSAKAQVDGGAVMGFGHALFEETIYQDGQLQNGDPFQYRLPVMKDLPELFYSSMLE
                     KCDGPGPFGSKGMAQTSIVTVGPAIGNAVYDALGVRVRSLPITPEKILRAMGKIE"
BASE COUNT          771 a         1181 c         1047 g          740 t
ORIGIN      
        1 gaaacgttga agttagccac ggcaacacgg atcttatccg cggccaggac cgggccttcg
       61 cccaaacaaa acgaggcgat cgtcaaaccg cccaaccact gccacagcat catcttattc
      121 ctccttggac tgataggccg ccgccgcttc aagaatggac tgcgcgatcg cgccataggc
      181 gccgcaacgg cagatattgc cgctcaacca ctcggcgatc tgttcgcggg acggattggg
      241 attctccatc aacagcgcct tggccgccat gatctgcccc ggagtgcagt aaccgcactg
      301 ggcgccgatg ttgcggataa aggcttcttg gagcggatcg agtttatcac cgtgagcaac
      361 gccttcgatg gtgcgcaccg atttctcatt ggcttcaaag gccaagtaat tacacgcact
      421 caccgcgcga ttatcgacca acaccgtgca cgccccacag acttccgact cgcatgagcg
      481 tttcgtgccg gtaaggtgaa gacgctgacg caagaattca agcaaggtct cttcgatggc
      541 aacctcgccc gaccaagctt ggccgttaac ggtaactgtg agattgaatg acgattgggt
      601 ttccatagaa tccgctaatc ttccgggatc ctttcacccg cggcctgcgc caacgcttct
      661 ccgagcatca ctccggtcat gtacaatttg tattccacgc tgcccagcaa atcatcgacg
      721 ggacgcaaaa catcgcgcaa atattttttc tgatcggcaa atattctttt cgtatccgtc
      781 agcggcacgc cgttaacctt ctcctccaat gccgtcagcc gctgcgcctt cggtccaata
      841 caacccacgg ccaatcgcgc ctcggcaacc ttctcgttat cgattcgtag cgccactgca
      901 acaccgagcg tcggacgctg ataacgatgc agccgctggt atgcgctcgt catcccggca
      961 ggcagcgacg gaatctcgac ttcgagcaac agctcgccgg cttcgagcgc cgtggcatac
     1021 atgtcgacga aaaaatcatc gagtgccaag cggcgctcgc ctttagcgtt gccaatcgtc
     1081 accgaagcgt tatgaaccag caaaaccgtg cccggatcgg aatgtggatc gttgaacgcg
     1141 agattgccgc cgagggagcc ttgattgcgc acccgtatat tggcaacctg cgactcagcg
     1201 tatgcaaagg acggcgcccg ttcgcggatc gccgcgttcg tcgccaactc atgatgcgtc
     1261 aagcaagcgc cgacgtgcaa cgtgccgttt cgtaaatcga gccgccgcag ccgctcgatc
     1321 ttcttcacat cgatcaaaat ttccgcatcg atcaggccgt ttcgcagcag cagcaacaat
     1381 tcagcgcctc cggcatagag cttggccttg tcgccaaact ccgcgagcgc ttgggaagct
     1441 tctcgaactg aagttggctg aacaagcttc aaaggattca acatgctagc agctcatccg
     1501 actcatcaaa cacgaaatcc gaaactattc tatctttccc atcgcccgca aaatcttttc
     1561 cggcgtgatc ggcagcgagc gcacgcgcac gccgagcgcg tcgtagactg cgttgccaat
     1621 cgccgggccg acggtgacga tggaggtctg ggccatgccc ttggagccga acggccccgg
     1681 cccatcgcac ttttccaaca tcgacgaata gaaaagctcc ggcagatcct tcattaccgg
     1741 caggcgatat tgaaaaggat cgccgttctg caattggccg tcttgataaa tcgtctcttc
     1801 gaacagcgca tggccgaagc ccatcaccgc gccgccgtcc acttgcgcct tagccgagga
     1861 atagtgaatg gttttgccgg cgtcggcgat gaccgcgtag ccgagaatgc gcagctcgcc
     1921 ggtgtcgcgg ttcacttcga gatcgaccgc cgcgccgctg ggcgcccagt gatccatgcc
     1981 ggcgaacgcc gagtccttca ccatcggcgg cacgctatga tagccgaccg atttcaagac
     2041 cacgctggcg ccgaccgcgc gaatgatttc gttgatcgaa aaactcgact cgccccagca
     2101 cagccacccg tcgacgatct gccagtcctt ggcttcgccg cctttgacct gcgccgccag
     2161 cccgagcaaa tcctcctgca atgtcttaca ggcattgtgc accgcgttgc ccatctgcat
     2221 ggtcgtgcgc tgggcgctca caccggcgaa gggcaaattt accgcggtgt ccggcgtgcc
     2281 cacatgtatc tggctctgcg gcaggttcaa ggtctgtgac gccaccacgc tgaatagatt
     2341 atgcgtgcct tggccgatct ccggcgcatt gtgttggatc gtaagaacgc cccgcgcgtc
     2401 cagcgtgacc agcgcatagg cccggccacc gccttgagaa ccatggcgca ggctcaaagc
     2461 catgccgcgg ccgcgcgccc agcgcggccc gttcaagctc ggcgcaggat tcgcttggcc
     2521 atcccaagcg atgccggcga tcgcttcaga catgagatcg agataatccg tgtccatgtt
     2581 gggcgtgccc ttacagacga aatcgccgcg cagcagcaca ttcttcttgc gaaactcgta
     2641 ggggtcgatg ccgagctggc gcgccacgct gtccatagcg cactcgatgc cccatgtggt
     2701 ttggatcttg ccggtggcgc gagtgtggcc ggcgggaacc ttgttggtgt agacgcactt
     2761 gccgcggatg cgcaggtggg gaatgcggta gcagccccag gcggaaatca ccgagttgtg
     2821 caccgcggtg gcgccgccgg tggtgtaggc gccggtgtcg acgaccagat cgacatcgag
     2881 ggcggtcaaa gtgccgtccg ttttgacgcc gactcgagct ttgaaaactt cggcgtggcg
     2941 cgcgttctga cgaaagctct cttccgccga cgggatcaag cggatgggac ggccgatctt
     3001 gcgcgaaagg aagagcgcgg cgaaatgcgc cggcgtcggt atcttcgagc caaagccgcc
     3061 gccgacgtag ggcacgcgca cgcgcacttt atcaggctcg atgccgagaa agtgcgcaaa
     3121 ctccgccgcg tcgcgggtcg gcgagctggt cggcagccaa atgttcgcct cgccgttgac
     3181 atagtgggcg agaaatccgc ccaccggttc catcggatgg tgaaacatcg acggcgacga
     3241 gtaagtctcc tcgaatatcc ggtgcgcttg cttaaagccc tcgtcgacgt caccccagac
     3301 caggccatct tcgaccaaaa gattgccgcc acgatcttcg tgcaacagcg gcgcgcccgg
     3361 ttccattgcc gcaaccgcat cgaatactgg tggcaacgga tcatagtcga cttgaatttt
     3421 cgccaacgcg cgctccgccg tgcgcctatc ctcggcaacg accagcgcca ccagatcgcc
     3481 gtcgtaacga actttattaa tcgcgacaaa gtcctggtcg tcggtaattt ttaaatgctc
     3541 atggggcgcg agcttcagtc gcgggttgac gccatcaaga tgttcccgat cgacgacgcc
     3601 taagactccg ggtattttaa gcgcttcggt acaatcgatg cggcggatct tcgcgtgcga
     3661 gtatgggctc aacagcgtcg cgccgtaggc cgtgttgggc agatcgggca gatcttcgat
     3721 gtactccgcc cggccagag
//
LOCUS       DASQKS010000537         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3247242, whole genome shotgun sequence.
ACCESSION   DASQKS010000537 DASQKS010000000
VERSION     DASQKS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247242"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VE170_13725"
     CDS             complement(<1..297)
                     /locus_tag="VE170_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16549.1"
                     /translation="MRLRLALLFLAANLILADPSHGQSPYFEGKTMTIIVGTGAGDLY
                     DLYARAVALFMGKHLPGNPNILVQNMPGAGHMIAANYVYNIAKPDGLTLGAINAG"
     gene            complement(366..1379)
                     /locus_tag="VE170_13730"
     CDS             complement(366..1379)
                     /locus_tag="VE170_13730"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16550.1"
                     /translation="MVLVSALWAVRVSAQAEPFYKGKQIRIITGATAGGFYDRWGRLL
                     ARAMPKYIPGQPDIIVQNMPGAGSLVAVNYVNGVAKPDGLTTVMPNSNVYLEQLSGHK
                     EVRFDLRKFAIVGSQEKNYMLLYMRADAPYKTIGDIIKAKEAPKCGSTGVGSAGYVLD
                     RVLDVVLGAKINTVMGYPGGNEIDLAVEKGEIQCRGNTILPHFGREPFDTWHKKGFDR
                     HLIQTARKRDPVVAEAPTIYELMDQYKVTESNRRLANVLLGGAEFGRFMLVAPGTPPE
                     RVKMLREAYARAMKDPELIAEAKKGRMDMDPSTGEELQAQMQEIMDQPPDVIERMKKI
                     LSE"
     gene            complement(1502..2617)
                     /locus_tag="VE170_13735"
     CDS             complement(1502..2617)
                     /locus_tag="VE170_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16551.1"
                     /translation="MALFFHSDDVDQMIPIKEAVRITEDALRDMISPTGVCAPRKRLN
                     LHRKVGEETFDTVLNIYAGGSAHYRAVGAQVALHRKAIAGNTQKRPPFNPDQTELALI
                     YDSDTGSLLGVMAHRPRDFTGVADLRTPATSLAGLDLFARADARRVGIYGSGQQAWAT
                     FAGLCEMRKIESAKVYSPTPAHREGFAKKISARTGVTVQPVEKPELAAKDVDIILCMT
                     NTNVPVIDGSWLEQGQYIISVIGSNIELVKSGNLAAPRREIDDGTLKRCDFIVALSKA
                     QAIDSQQGDIYWPVQDGVITWDKVVEISDVLAGKVKGRTNDKQIILYKNQGGQGIIDI
                     ALAKKCYDLAQQHGKGYEMHIEPRLNWWVQGGRAETW"
     gene            complement(2645..>3297)
                     /locus_tag="VE170_13740"
     CDS             complement(2645..>3297)
                     /locus_tag="VE170_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ16552.1"
                     /translation="QLHTRVTRARGMEKRFSLTPNDVLGQGGVIEGFKTMRGRGKVGF
                     FGFSGLGETPALHELVDSGEFHGFQCYYNLLNPSSGQPVPKNFSAQDYGLILDRAAAK
                     GMGAFIIRVLAAGALTADPSAGGGGSGQTLSPGSDYPLDLKRAEKVKTALGIAGKELT
                     QAAIRFGLMHQTTAAVLVGFSNTHHVDDAVACSNLPSLTGEQIGKMRNLWETDFSD"
BASE COUNT          653 a         1020 c          900 g          724 t
ORIGIN      
        1 tcccgcgttg atcgcgccca aggtcagacc atcgggctta gcgatgttgt agacataatt
       61 ggccgcgatc atatggcccg cgcccggcat attctgaacc aaaatgttgg gattgcccgg
      121 cagatgcttg cccatgaaca acgcgaccgc gcgcgcgtac aggtcataaa gatcgccggc
      181 gccggtgccg acgatgatgg tcatggtctt tccctcgaag tatggggatt ggccatggga
      241 cggatcggcc aggattaagt tggctgccaa gaacaacagg gcaagtctga gcctcatcgt
      301 tttatcctcc taatcgcttc tattgaacca tatgaacctg gggaaccgtt gcaccgagtt
      361 attttctact cagataagat ctttttcatt cgctcgatca catcgggcgg ctgatccatg
      421 atctcctgca tctgcgcttg cagttcctcg ccggttgaag gatccatatc catgcgcccc
      481 ttcttggctt cagcaatcag ctcgggatcc ttcatcgccc tggcataggc ttctcggagc
      541 atcttgactc gctccggcgg cgttcccggc gcgaccagca taaagcggcc aaactcagcg
      601 ccgccaagca agacgttggc gagccggcga ttcgactccg tgactttgta ctggtccatc
      661 agctcgtaaa tcgtcggcgc ctcggcgacc accggatcgc gtttgcgtgc ggtttgaatg
      721 agatggcgat cgaaaccttt cttatgccaa gtgtcgaagg gctcgcggcc gaaatgcggc
      781 aagatggtgt tgccgcggca ttgaatctcg cccttttcca ccgccaggtc gatctcattg
      841 ccgcccggat agcccatcac ggtattgatc ttcgcgccaa gaaccacgtc caaaactcgg
      901 tcgagaacat agccggcgct gccgacgccg gtgctgccgc acttcggcgc ctccttggct
      961 ttgatgatgt cgccgatggt cttgtacggc gcatccgcgc gcatatagag caacatataa
     1021 ttcttttcct gcgagccgac gatcgcgaac ttgcgcaagt cgaagcgcac ttctttatgg
     1081 cccgatagct gctccagata gacgttgctg ttaggcatga ccgtggtcag accatcgggc
     1141 ttggccacgc cgttgacata attaaccgcc accagcgagc ccgctcccgg catgttctgc
     1201 acgatgatat cgggctgtcc tggaatatac ttgggcattg cccgggccaa aagccgtccc
     1261 cagcggtcat aaaagccgcc cgccgtggcc ccggtaataa tgcgaatctg tttgcccttg
     1321 tagaaaggtt cggcctgcgc actgacccgg acagcccaga gtgcgctaac cagcaccatt
     1381 gccacaacaa caattccgaa tcgcaactga attaaatctg tcaatttcat ccttcgccct
     1441 catccttctt gttttctccg accttgaacg cttgaacctt tgaacccttg aacagtaaat
     1501 ttcaccaagt ctccgcgcgc ccgccttgca cccaccagtt gagccgcggc tcgatgtgca
     1561 tctcgtagcc cttaccatgt tgctgcgcca aatcgtaaca cttcttcgcc agcgcgatat
     1621 cgatgatgcc ttggccgcct tggtttttgt agagaataat ctgcttatcg ttggtgcgcc
     1681 ctttgacttt tccggcgagc acatcggaga tttccacgac tttgtcccaa gtgatcacgc
     1741 catcctgcac cggccaataa atatcgcctt gctgggaatc gatggcctga gctttcgata
     1801 gcgcgacgat gaagtcgcac cgtttaagcg tgccgtcgtc gatctcccgg cgcggcgcgg
     1861 ccagattgcc gctcttgacc aactcgatgt tgctgccgat gacggaaata atgtactgcc
     1921 cttgctccag ccacgaaccg tcgatcaccg gcacgttggt gttggtcatg cagagaatga
     1981 tgtcgacgtc tttcgcggcc aactcaggct tttccaccgg ctgcaccgtc acgccggtcc
     2041 gagcgctgat cttcttggcg aagccttcgc ggtgcgccgg cgtcgggcta tataccttcg
     2101 cgctttcgat cttgcgcatc tcgcacagcc cggcaaatgt cgcccaagcc tgctggcccg
     2161 agccatagat accgacccgc cgcgcgtcgg cgcgcgcgaa tagatccaaa cccgccaagc
     2221 tcgtcgccgg ggtgcgcaga tcggcgacgc cggtaaaatc ccgcggccga tgcgccatca
     2281 cacctaaaag cgacccggtg tccgaatcat agatcagcgc cagctcagtc tgatcgggat
     2341 taaatggcgg ccgcttctgc gtgttgcccg cgatcgcctt gcgatgcagc gccacctgcg
     2401 cgcccacggc gcggtaatgc gccgatccac cggcgtaaat attgagcact gtgtcgaagg
     2461 tctcttcacc gaccttgcga tgcagattca agcgcttgcg cggcgcgcaa acacccgtgg
     2521 gcgatatcat gtcgcgcaac gcgtcttcgg tgatgcgcac cgcttctttg atcggaatca
     2581 tttgatcgac atcgtcggag tggaagaaca aagccataac gcatctcccg aaaatcaaaa
     2641 ctaactagtc gctgaaatcg gtctcccaaa gatttctcat cttacctatt tgctcaccgg
     2701 tcagcgacgg caagttcgaa caggccaccg cgtcatcgac atgatgggta ttggaaaatc
     2761 cgacgagcac cgcggcggtc gtctgatgca tcaagccgaa acggatggcg gcttgcgtca
     2821 gctcttttcc cgcaatgccg agggcggttt tcactttctc ggcgcgcttc aggtcgaggg
     2881 gatagtcgga gcccggtgag agcgtctggc cgctgccgcc accgccggcg ctgggatcgg
     2941 cggtcagcgc gccggccgct agcacgcgaa tgatgaaggc gcccatgcct ttggccgcgg
     3001 cgcgatcgag gatcaatccg taatcttggg cgctgaaatt cttcggcacc ggctggccgg
     3061 agctggggtt gagcaggttg tagtagcact gaaagccatg aaactcaccg ctgtcgacta
     3121 gctcgtgcag cgccggggtt tcgcccagac cgctgaaacc gaagaagccg accttaccgc
     3181 gcccgcgcat cgttttgaaa ccttcaatga cgccgccttg gccgagcacg tcgttgggcg
     3241 tcaggctgaa acgcttctcc atgccgcgcg cccgggtgac gcgggtatgc agctgga
//
LOCUS       DASQKS010000538         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4901819, whole genome shotgun sequence.
ACCESSION   DASQKS010000538 DASQKS010000000
VERSION     DASQKS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4901819"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VE170_13745"
     CDS             complement(<1..1607)
                     /locus_tag="VE170_13745"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYJ16553.1"
                     /translation="MDAVAISLALLQDSQVEVTLKALGRSYLERGRRILFERHREGAG
                     GIEVAAAYSTVMDHLIRYLFSTLGAELLPRFRGQGTGLALIAQGGYGRGELSPYSDVD
                     LIVLHNGRVNSFFQALNERLLHTLWDLGLDVPPAMRRISDCVSLGESEMKIRTALLDA
                     RFLCGDFQLFGEFSKTLDNKLFNKGIGRFVREKLDETVFRHKTYGGSVYLLEPEVKEG
                     LGGLRDIQTARWIARVKSKAKDLDALAAHGVISSSDVVGLRESQDFLLRVRNEMHFAT
                     GKHQDQLTFEQQEQVSEALGFQSEGTLRGVEVFMRAYYLHAMQISRLSELIIHRVTDC
                     DKPRFTDKLVGRTLREGVRISKGSLTVTKPEVLKNDPTNLIRLFDEGQKYHCRLSHET
                     RELLRSHAGLIDEEFRSSAAANQPFFSILGWKDAVYETLLEMHRSGVLGAFIPEFGRL
                     LCMVLHDAYHTYTVDEHSLMLVQEIERLRAGNYEAALPLLTQVAREAEKIELLYLGMM
                     FHDIGKGFGGGHSEIGAKMVRPIARRLG"
     gene            complement(1616..2695)
                     /locus_tag="VE170_13750"
     CDS             complement(1616..2695)
                     /locus_tag="VE170_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYJ16554.1"
                     /translation="MLELSQDAKYEVRRFKDDPAKGIAPRMYIDIRGARLAMNSTQPA
                     PMDDGLVRQVRVSQYSAEVVRVVLDLTRVQNHNAFMLLDPRRLVIDLHGQQTPEPAVI
                     KEGAAKAENTPRLGAGRDKQAALPLANATGIHKIVIDPGHGGKDPGAVGVGGIMEKDL
                     VLSIAKKLAVKLKNEMGVQVVLTRNDDRFVALEDRTHRANAEDADLFISLHTNASTNG
                     EARGVETYYLDNTTDEAALRLAARENGGARKPVSDLQFILSDMTQNMKLEDSITLAHR
                     LQQSVVGGMTKAVGEVRDLGVKQALFYVLVGARMPSVLVEMFFITHHLEGAAMNREAN
                     QDTLVNSLFEGIQKYGQSNMTGKPL"
     gene            complement(2968..>5523)
                     /gene="mutS"
                     /locus_tag="VE170_13755"
     CDS             complement(2968..>5523)
                     /gene="mutS"
                     /locus_tag="VE170_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYJ16555.1"
                     /translation="TPMMQQYLQIKERYQDAILFFRLGDFYEMFFEDAHVASKVLDIT
                     LTSRNKSDDGAVPLCGIPYHAAQPYIQKLLEAGHKVAVCEQVEDPALAKGVVKREVVR
                     VITPGTVTADEALDGRGNNFLAAVCQGETAYGLAVSDITTGEFRCTEVADEAAFLDEL
                     GRVRPSEILLAGRDSRLRERIYKEYPAIHLTMVRDEAFIDLPGAEPTRGADLPVAGAR
                     AAAAIVDYLNANIPEAVKLLRDLHSYQVANFLLLDETTRVNLELIATTQGERRGSLLA
                     ILDRTQTAIGARRLRQWLLYPLLDEAAIGARYDAVQELAEGFASREELKLRLAKIQDL
                     ERLSGRVLAGSATPKDLVAIKQTLGAAGGVRAQTAGFTAPLLVDLSAALAELPEVVGL
                     IERAIVDDPPFAFKEGGFIRAGFDGELDEIRGMRSHAKEWIAQFEAGERKRSGITSLK
                     VRYNRVFGYYIEVTNSHLPAVPADYIRKQTLANGERYITPELKEYEAKVLNSESLMEK
                     LEANLLARVREQAALHYAALKTMSNALAILDVLTALADVAESQRFVRPRVDGSTVLNI
                     REGRHPVVENAVGRGSFVPNDCKIDAESEQIMMLTGPNMAGKSTYMRQNALIVILAQM
                     GGFVPAAEAQIGIVDRIFTRIGAADSLSRGESTFMVEMKETANILHHATRRSLILLDE
                     VGRGTSTFDGISIAWAVAESLHECHERPRTLFATHYHELTELALANERIKNFNFAVKE
                     WRGEIIFLRNLVAGAASHSYGIHVARLAGMPAPVIERAKAILAQLEESQSVAPSSVVK
                     RRGEGSAEMPVQMALFSAADNRLRQRLTQLDVGNLTPIQALNVLHELSEEAKK"
BASE COUNT         1122 a         1707 c         1556 g         1138 t
ORIGIN      
        1 cccaggcgcc gggcgatggg gcgcaccatt ttggcgccga tttccgagtg accgccgccg
       61 aaacccttgc cgatgtcatg aaacatcatg cctaagtaga gtaattcgat cttctccgcc
      121 tcgcgcgcca cttgcgtcag caagggcagc gcagcttcat agttgccggc gcgcagccgt
      181 tcgatctcct ggaccagcat gagagaatgc tcatccaccg tgtaggtgtg ataagcgtcg
      241 tgcagcacca tgcacagcag tcggccgaac tccgggatga acgccccaag cacgcccgaa
      301 cgatgcatct ccaagagcgt ttcatagacg gcatctttcc acccgagaat cgaaaaaaac
      361 ggttggttgg cggccgccga agagcgaaac tcttcgtcga tcaggccggc gtggctgcgc
      421 agtagctcac gcgtctcatg actcaagcgg cagtgatact tctgcccttc gtcgaacagg
      481 cgaatcagat tagtcggatc gtttttgagc acctccggtt tggtcacggt aagagaaccc
      541 ttggaaatgc gcacgccttc gcgcagcgtg cggccgacca acttgtccgt gaaacgcggc
      601 ttgtcgcagt cggtaacccg atgaataatc agctcggaaa gccggctgat ctgcatggcg
      661 tgcagataat aagcgcgcat gaacacttcg acgccgcgca gtgtcccctc gctttgaaat
      721 cccaaagcct cgctgacttg ttcctgctgc tcgaaggtca gctggtcctg atgcttgcca
      781 gtagcaaagt gcatttcgtt gcgcacccgc aacagaaaat cctgcgactc tctgagcccg
      841 accacatcgc ttgagctgat cacgccgtga gccgcgagcg cgtctagatc cttggcctta
      901 cttttcacgc gggcgatcca gcgggccgtc tggatatcgc gtaaaccgcc cagaccttct
      961 ttgacctccg gttcgagcaa atagaccgat ccgccatagg ttttgtgacg gaaaacggtt
     1021 tcatcgagtt tttcacgaac gaatcggccg atacccttat tgaacaattt gttatcgaga
     1081 gtcttactga attcgccaaa cagctggaag tcgccgcaca gaaagcgcgc gtcaagcaac
     1141 gccgtgcgga tcttcatctc cgactcgccc aaactcacgc agtcggagat cctgcgcatc
     1201 gccggcggga catccaaacc caaatcccac aacgtgtgta acaatcgctc gttgagagct
     1261 tggaaaaacg aattgacccg accgttgtgc agaactatta aatcgacatc ggaatacgga
     1321 ctaagttcac cccggccata gcccccttga gcgatcaacg ccaatccggt tccctggcca
     1381 cgaaaacgcg gcaggagctc ggcccccaag gtgctgaaca gatagcgaat gagatgatcc
     1441 atcaccgtcg aataagccgc cgcgacttcg atgccgccgg cgccctcgcg atggcgttcg
     1501 aacaaaatgc gccggccacg ctcgagatag ctccggccca gcgctttgag ggtgacttcg
     1561 acctgactgt cttgaagtaa ggctaatgaa atagcgactg cgtccataat tgatattata
     1621 gcggtttacc cgtcatattc gactgtccat atttttgaat gccttcgaac agcgagttga
     1681 ccagggtatc ttgattggcc tcgcggttca tcgccgctcc ttcgaggtga tgggtgatga
     1741 aaaacatctc caccaagaca ctcggcatgc gcgcgcccac caagacataa aatagcgcct
     1801 gcttgacccc caagtcgcgc acctcgccga ccgctttagt catgccgccg accaccgact
     1861 gctgcaaacg gtgcgctagc gtgatcgaat cctcgagctt catgttctga gtcatgtcgc
     1921 tcaggataaa ttgcaaatcg gaaaccggct tgcgcgcgcc gccgttctct cgcgccgcca
     1981 gccgcagcgc cgcctcgtcg gtggtgttgt cgagataata ggtctcgacg ccgcgcgctt
     2041 cgccattggt gctggcgttg gtatggaggg aaataaacag atcggcatcc tcggcgttgg
     2101 cgcgatgagt gcggtcctcc agggcgacga agcggtcgtc attgcgggtc aagaccacct
     2161 ggacgcccat ttcattcttg agcttaacgg caagcttttt agcgatgctc aaaaccaagt
     2221 ctttttccat gataccaccg acaccgaccg caccgggatc cttgccgcca tggcccggat
     2281 cgatgacgat cttgtgaatg ccggtggcgt tagccagcgg cagcgccgcc tgcttgtctc
     2341 gcccagcgcc gagccgggga gtattttccg ccttggccgc cccctccttg atgactgcgg
     2401 gctccggcgt ttgctgcccg tgtaagtcga tcaccaaacg acgcgggtcg agcagcatga
     2461 atgcattgtg attttgcact ctggtcagat ccaacaccac gcgcacaact tcggcgctgt
     2521 attggctgac gcgcacctga cgcaccagac catcgtccat cggcgccggc tgcgttgagt
     2581 tcatcgccaa gcgcgcgccg cggatatcaa tatacatgcg cggcgctatg cccttggcag
     2641 gatcgtcctt gaaccggcgc acttcgtatt tggcgtcttg cgatagctcc aacatgacgc
     2701 gggtgaaatt tctccaggat aagaaacgca ggccggtgac ttgaacttga tccgaagccg
     2761 gcggcgacga ttgattttct ccgtgcctgg gcgaggccga ttgtgcactg gtcgaaatcg
     2821 catggcgcga cgccggaacg ctgcggactt gcgctgaaag cctgctctca gaccacagcc
     2881 acaatgccgc caaggcaatt aacccgatgc taacctgact acgtggcttc ataaccttcg
     2941 ctatcgctcc cggctaaatt ccgccgatca ttttttggct tcttcgctca actcatggag
     3001 gacgttcagc gcctggatcg gcgtcaggtt accgacatcg agttgggtca agcgctggcg
     3061 cagccgatta tcggcggcgc tgaacagcgc catttgcacg ggcatctcgg cggatccctc
     3121 accgcggcgc ttgaccacgg agcttggcgc caccgactgg ctttcctcca actgcgctaa
     3181 aatcgcctta gcccgctcga tcaccggcgc cggcatgccg gctaaacgag ccacgtggat
     3241 accgtagctg tggctcgccg ctcccgcgac cagattgcgc agaaaaataa tctcgccgcg
     3301 ccactccttg acggcgaaat tgaaattctt gatgcgttca ttggccaggg ccaattcggt
     3361 gagctcgtga tagtgagtgg cgaacagtgt gcgcggccgc tcgtggcatt cgtgcaacga
     3421 ttcggccacc gcccaagcaa tggaaatgcc gtcgaacgtg ctcgtgccgc ggccgacttc
     3481 gtcgagcagg atcaggctgc gccgggtcgc atggtgcaag atattcgccg tttctttcat
     3541 ctcgaccatg aacgtcgact cgccgcggga gagcgaatcg gccgcgccga tgcgcgtaaa
     3601 gatccggtcg acgatgccga tctgcgcctc ggccgccgga acgaaaccgc ccatttgggc
     3661 gaggataacg atcagcgcgt tctggcgcat gtaggtcgac ttaccggcca tattcggccc
     3721 ggtaagcatc atgatctgct cggattccgc atcgatcttg caatcgttgg gcacaaagga
     3781 gccgcggccg acggcgttct cgaccaccgg atggcgcccc tcgcggatat tcaaaaccgt
     3841 gctgccatcg acgcgcggac gcacgaagcg ctgcgactct gccacatcgg ccaacgccgt
     3901 gagcacgtcc aagatcgcca gcgcattgct catcgttttg agtgcggcat agtggagcgc
     3961 cgcctgctcg cgcacccggg ccaacaaatt cgcctcaagt ttctccatga ggctttccga
     4021 gttgagcacc ttggcttcgt attcttttag ttcaggggtg atatagcgct cgccattggc
     4081 cagggtttgc ttgcgaatgt aatccgccgg cacggccggc aaatggctgt tggtcacctc
     4141 gatgtagtaa ccgaataccc ggttgtagcg gactttcagt gacgtaattc cactgcgctt
     4201 gcgctcgccg gcttcgaact gggcaatcca ctccttggcg tggctgcgca tgccgcgaat
     4261 ctcgtcgagc tcgccatcga aaccggcgcg aatgaagccg ccttctttga aggcgaaggg
     4321 cggatcgtcg acgatcgccc gctcgatcag cccgaccact tcgggcaact ccgcaagagc
     4381 cgcgctcaag tcgaccaaca gcggcgcggt gaaaccggcg gtctgggcgc gcacgccgcc
     4441 ggcggcgccg agagtctgtt tgatcgcgac caaatccttg ggcgtcgcgc tgccggccaa
     4501 cacccggccg ctgagtcttt ccaaatcctg gatcttcgcc aaacgcagct tgagctcttc
     4561 ccgtgatgcg aacccctcgg cgagttcctg cacggcgtcg tagcgagcgc caatcgccgc
     4621 ttcatcgagc aatggataga gcagccattg gcgcaatcgg cgcgcgccga tggccgtttg
     4681 cgtgcggtcg agaatcgcca gcagtgaacc gcgccgctcg ccctgagtgg tagcgatgag
     4741 ctccaaattg actcgggtgg tctcgtcgag caacaaaaaa ttggcgacct gataagaatg
     4801 cagatcgcgc agcagcttca ccgcttcggg aatattagcg ttgagataat caacgatcgc
     4861 cgccgccgct cgcgcgcctg caaccggcag atcggcgccc cgggttggct ccgctccagg
     4921 caaatcgatg aacgcctcgt cgcgcaccat ggtcagatgg atcgccggat actctttgta
     4981 gattcgctcg cgcaatcggc tgtcgcgccc ggccagcaag atttcactgg gccgaacgcg
     5041 gccgagttcg tcgagaaacg ccgcttcgtc ggcaacctcg gtgcaacgga attcgccggt
     5101 ggtgatatcg ctgaccgcga gaccgtaagc ggtttcgccc tggcataccg ccgcgagaaa
     5161 attattgccg cggccatcga gcgcttcatc ggcggtcacc gtgcccggtg taatcacccg
     5221 caccacttcg cgtttgacca cgcccttggc cagggccggg tcttccacct gctcgcatac
     5281 tgcgactttg tggccggctt cgaggagttt ttgaatgtag ggctgcgcgg cgtgatacgg
     5341 tatgccgcat aacggcaccg caccgtcgtc gctcttgttg cgcgaggtta gcgtgatgtc
     5401 gagaactttt gacgcgacgt gggcatcttc aaaaaacatt tcgtaaaaat cgccgaggcg
     5461 gaagaacaag atggcatcct gataccgctc cttgatctgt aggtactgct gcatcatcgg
     5521 cgt
//
LOCUS       DASQKS010000539         8767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3247618, whole genome shotgun sequence.
ACCESSION   DASQKS010000539 DASQKS010000000
VERSION     DASQKS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247618"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VE170_13760"
     CDS             <1..1197
                     /locus_tag="VE170_13760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16556.1"
                     /translation="KKLGTIALLYFIQGSPAAILWEVLPVYFRINGVSLRAIGGLRLL
                     ELPYSLKVFWSPLVHRFGDRRWWIVACMLGMAAVLFALPFVNVAAVGVIVLVLILALT
                     TLSATQDVAIDSYSVGLVDRAEEGAANGVRASAYRVALVFVGGGAVFLAGLLAWNSLF
                     VLAGVVFAALAFAVLAVPQLSLPEAAREHWLAGFTGWAGTWKVIPLIMFVLTYKLGEF
                     AIGPMVKPFWVDYGKTIWPVQEDLMFQIGMFPTTFGIVLSVVGALMGGAFISRYGIFH
                     GVWFLGLLQAVSNLGYSLVVWMDLGRFGLYGASMFESLSGGLGTAAFLAFLMNVCQKE
                     HATVQYAFLSSVFSLTGRLIGAISGLGAEKYGFASYFAITFLLSTPAYLLLPWVKDWI
                     HDERRE"
     gene            complement(1200..2159)
                     /gene="senB"
                     /locus_tag="VE170_13765"
     CDS             complement(1200..2159)
                     /gene="senB"
                     /locus_tag="VE170_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HYJ16557.1"
                     /translation="MNIQLITPAPLKLNNGNKITAVRWATIFKKLGHRVQVRQSYDGK
                     SCDVLIALHALRSADSIRRFHEAQPRKPIIVVLTGTDVYRDIHENKKAQRSLELANRL
                     VALQSMALCELPKKFQAKTRVIYQSAATVRGKTKNRNHAFNVCVIGHLRKEKDPLRTA
                     LAVRNLPRQSRVMVTHVGQALDRRIARSARAEVKRNPRYRWIGQLSHRQTRELLAKSH
                     LVAITSRMEGSSNVLSEALASGVPVVATKISGLMGTLGKRFIGYYPPADTDKLQRLLL
                     RAESDGKFYRELKRQCRGFAHLVRPERERNAWRRLLREIELLE"
     gene            complement(2168..3598)
                     /gene="senA"
                     /locus_tag="VE170_13770"
     CDS             complement(2168..3598)
                     /gene="senA"
                     /locus_tag="VE170_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HYJ16558.1"
                     /translation="MSKTIASATLGPSATMGARQLIATMREFRDRLLSLVADLDDRQM
                     IGPRLAIVNPPLWEIGHVAWTQEFWALRNLRGEKPLLPESDRLYNSTDVAHDSRWELS
                     LPNRADTLAYMNEVLERCIAGIDSKSDLSPDEFYFYWLTTFHEGMHAEALAYTRQTLG
                     YPPPQFAKAFLSTDDSDLRACPGDVEIPGGKFTIGAAKDMPFIFDNEKWAHEIEIKPF
                     RIARSPVTNGEFLQFVEAGGYRERMHWSDEGWQWLQSGGAPQLEKSFAKFFNKNVTEP
                     MEIAAFTETLDHPVYWQQFDNTRWQQRVYDRYVLLNENLPVVHVSWFEAEAFCNWAGR
                     RLPTEAEWEVAAAGEPTANGIALAPRRRHYPWGDNRPEPQHASLDWRGGGLVEVAMHE
                     AGDSAFGCRQMIGNVWEWTADNFLPYPGFSADSYKEYSNPWFGTHKVLRGGCWATSSL
                     LIHNGWRNFYTPDRRDVWAGFRTCAK"
     gene            complement(3662..4804)
                     /locus_tag="VE170_13775"
     CDS             complement(3662..4804)
                     /locus_tag="VE170_13775"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HYJ16559.1"
                     /translation="MSAAKTFQRRGIVEGFFGPMWSMGHRRRLFAFGAARGMNTYLYA
                     PKDDPYHRKLWRRPYPRAQWHALTRLIRAAQKSQIDFVYGFHPGEGLYFCDDHMVRLL
                     LHKAQRFYDVGVRTFTVLFDDIPSRLSDARDRRAFKNSLARAEGTWMAQIMAAQPAHW
                     RGVEWWICPTYYSEDALLERVFGKFEPNFLETLGLYLPANVPCFWTGPSVVSKTIALS
                     HVKRIGKMIERPLLLWDNYPVNDLSMSDELHLAPLTGRDPRLAEHVYGYLNNPLLQEE
                     LSYIPLATCFDYARAPAKYRGDLTWTRIVRQRFGAEALPHWRALRSYAEASITAKKDR
                     RLLQISPAETRRLRLSFAYIERNRGRRWAQELAPWRPAIAKLIAGL"
     gene            complement(5175..6044)
                     /locus_tag="VE170_13780"
     CDS             complement(5175..6044)
                     /locus_tag="VE170_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16560.1"
                     /translation="MTTAEKIKPEDRFVTVDGLKLRYVEAGSGPSVLFLHGASLGSSA
                     DVFQRNLGPFAKAGFRAIAFDYPGFGLSDVPAKQSTAQQRNSIPKFIDAAGLGKTALI
                     AHSRSGGFAIQLALEDPSRYSHVIILGTGSLLPLQTEAQVGRYEAVQARVDKEMAEKE
                     PTVEDARKLLQADTFNHSLISDEDVALRHRSMIGRNFKAHQDRQNHEAPAAPAGASTP
                     AKPLWERLADLKMPLLMIFGREDRAHAGERAELLKKQQPHLNLHIVNGCKHMVHWDAF
                     DDLMRLGVPFLKS"
     gene            complement(6580..7059)
                     /locus_tag="VE170_13785"
     CDS             complement(6580..7059)
                     /locus_tag="VE170_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16561.1"
                     /translation="MNSAPVSPISAPLPPVPPVVQTRTDPFEQSKALFSQGQYEAAII
                     ENQKIFQDRKGSPDVALFNMGMISAHPTNPKKNYPRALSSFRTLVKDYPHSPMVDPAK
                     TWITVLEEHQKIADEKRSLTKEREALLQEKDKLKYTIEKSRQVDIDIEKRRRGTLQK"
     gene            complement(7134..7805)
                     /locus_tag="VE170_13790"
     CDS             complement(7134..7805)
                     /locus_tag="VE170_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16562.1"
                     /translation="MLILMVTVSGYSIIQLGKLSNAANIALKVDQRMIDYSEKLADAF
                     LSEVRYAGQFNVTHATQHYEQYMQFGSDFDRYTALLKPLAGSRGMGQRLARAEEFHGQ
                     YRQLFEKEVVYIKTKHAYAETRYREEKDRLVDYLLRENEAFKSDVQKSLQERIEYIEH
                     AAMKSQKITLAATLVLVVMGIFFACYLGAKIPQDGEQAPSAGPIARKRLLRFHYWLKG
                     FGATK"
     gene            complement(7850..>8767)
                     /locus_tag="VE170_13795"
     CDS             complement(7850..>8767)
                     /locus_tag="VE170_13795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ16563.1"
                     /translation="SFYSTETIDLRIEEAFLADLTHQAAIALYNSHLFEQTRNQAVEL
                     EKSNRIKDEFLGVISHELRTPLNIIMNCAEAFNMGIFGDITQEHAKGTEKIRVQASHL
                     LSLINSILEITKIESGRMAINAESINLDELIRDLESDYAMTSKEKNLAINWRIPADLP
                     NFVTDRMKLRQVAINLVNNAIKFTDQGSVDISFRSVPDEPFIEFSVSDTGIGMAEEFL
                     PHIFDKFNQIDNATTRNYSGAGLGLYLVKNFVDLLGGSVSVCSKLGEGTTFTVRLPII
                     LSSDVHDDDRLAGTGSSIVAPRPAMEVEL"
BASE COUNT         1791 a         2311 c         2563 g         2102 t
ORIGIN      
        1 aagaaacttg gaaccatcgc cttgctctat ttcattcaag gctcgccggc ggcgatcttg
       61 tgggaagtcc tgccggttta ttttcgcatc aacggcgtgt ccctgcgcgc cattggcgga
      121 ctgcgcctgc tggagctgcc ctattcactg aaagtcttct ggtcgccgct agtccatcgt
      181 ttcggcgacc ggcgctggtg gatcgtggcc tgtatgcttg gcatggccgc ggtgctgttc
      241 gcgctaccgt ttgtgaacgt cgccgccgtt ggcgtgatcg tgctcgtgtt gatcttggcg
      301 ctgacgactc tatcggcgac acaggatgtc gccatcgatt cctactccgt cgggttggtc
      361 gaccgcgcgg aagagggcgc ggccaatggt gtgcgcgctt cggcctatcg agttgccttg
      421 gttttcgtgg gcggcggtgc ggtatttctc gccggcctgt tggcgtggaa ttcacttttc
      481 gttctggccg gagtggtctt tgccgcactg gcgtttgccg tgttggcggt gccgcaattg
      541 agtttgcccg aagcggcgcg cgagcattgg cttgccggct ttaccggttg ggccggcacc
      601 tggaaggtga tcccgctgat catgttcgtc ctgacttata aattgggcga gttcgccatc
      661 ggaccgatgg tgaaaccgtt ttgggtcgac tacggcaaga cgatctggcc ggtgcaggaa
      721 gatctaatgt ttcagatcgg catgttcccg acgacgtttg gcatcgtgct cagcgtcgtc
      781 ggcgcattga tgggcggggc gttcatctca cgctacggca tctttcacgg cgtctggttt
      841 ctcggcctgt tacaagcggt gtccaatctc ggttactcgc tggtggtatg gatggatctt
      901 gggcgctttg gtctctatgg cgcgtcgatg ttcgagtcgc tttccggcgg ccttggcacc
      961 gccgcgtttc tggcgttctt gatgaacgtc tgccagaagg agcacgccac ggtgcagtac
     1021 gcttttctct cgtcggtgtt ttccctcacc gggcggttga tcggcgcgat cagcggactg
     1081 ggggcggaga aatacggctt cgccagctac ttcgcgatta ctttcttgct gtcgacgccg
     1141 gcatatttgc tattgccgtg ggtgaaagac tggattcatg acgaacggcg cgaatgaatt
     1201 tactcaagca attcaatttc ccggagcagg cgccgccaag cattccgttc ccgttcgggg
     1261 cgcacgagat gagcaaagcc gcggcattgc cgtttcagct cacggtaaaa cttgccgtcg
     1321 ctctccgctc tgagaagcag ccgttggagt ttatcggtgt cggcgggcgg atagtagccg
     1381 ataaagcgct ttcctagcgt tcccatcaag ccggaaatct ttgtcgcgac gacaggcaca
     1441 ccggaggcga gggcttcgga aagcacgttg gagctgcctt ccatcctcga cgtgatggcg
     1501 accagatggc tcttcgccag gagttcgcgg gtctggcgat gggagagttg gccgatccag
     1561 cgatagcggg gatttcgttt aacttcggcc ctggcacttc tggcaattcg tcgatctagc
     1621 gcttgaccga catgggtgac catgacccgc gactgtcgcg gcaggttgcg cacggctagg
     1681 gcggtgcgca gtggatcttt ttctttgcgc aaatgaccga tgacgcaaac attgaaggca
     1741 tggtttcgat ttttagtttt gccacgtacc gttgccgctg attgatagat cacccgagtt
     1801 tttgcctgaa actttttagg taattcgcac aacgccatgg attgtaaggc gacaaggcga
     1861 tttgctagct caagggatcg ttgagctttt ttattctcgt gaatgtcacg gtaaacgtcc
     1921 gtgccggtga gcacgacaat tatcggcttg cgcggttgag cctcgtgaaa ccggcggatc
     1981 gaatcggcgc tacgcagggc gtgtaacgcg atcaagacat cgcatgattt cccatcgtag
     2041 ctttggcgta cctgtacgcg gtggccgagt tttttaaaaa tcgtcgccca gcgcacggcg
     2101 gtgatcttgt tgccgttgtt gagcttcaac ggcgccggcg tgatcagttg aatgttcacg
     2161 aggtgattta tttggcgcag gttctaaagc cggcccaaac atcgcgccgg tccggcgtgt
     2221 agaagttgcg ccagccattg tgaatcaaca acgaggaggt tgcccagcag ccgccgcgca
     2281 ggactttgtg agtgccgaac cacggattcg agtattcctt ataagaatca gcgctaaatc
     2341 ctggataagg cagaaaatta tccgcggtcc actcccagac attgccgatc atctgacggc
     2401 agccgaaggc gctgtcaccg gcttcatgca tggcaacctc aacgagaccg ccgccgcgcc
     2461 agtcgaggct ggcatgttgc ggttcggggc ggttatcgcc ccaagggtaa tggcggcgcc
     2521 gcggcgccag cgcgatgcca ttcgccgtgg gttcgccagc ggctgccact tcccattccg
     2581 cttcggtggg caggcgccgg ccggcccaat tgcagaatgc ttcagcttcg aaccaactca
     2641 catgcaccac cggcaggttt tcgttcaata ggacatagcg gtcgtagaca cgctgctgcc
     2701 accgggtgtt gtcgaattgc tgccagtaga cggggtgatc gagtgtttcc gtgaatgcgg
     2761 ctatttccat cggctcagtg acgttcttgt tgaagaattt agcaaaggat ttctccaact
     2821 gcggcgcgcc gccagattgg agccattgcc aaccttcgtc gctccaatgc attcgctcgc
     2881 gataaccgcc agcttcgacg aattgcaaaa actcgccgtt ggttaccggg gagcgggcaa
     2941 tgcggaaagg ctttatctcg atctcgtggg cccacttctc gttgtcaaaa atgaacggca
     3001 tatctttggc cgcgccgatg gtgaacttgc cgccggggat ctcgacatcg ccgggacagg
     3061 cgcgtaggtc agaatcatcc gtcgataaaa aagccttagc aaactgcggt gggggatagc
     3121 cgagggtctg acgtgtgtag gcgagcgcct cggcgtgcat gccttcatga aatgtcgtca
     3181 gccagtaaaa gtaaaactcg tcgggcgata ggtcggactt cgagtctata ccggcgatgc
     3241 agcgttcgag cacttcgttc atgtaggcca atgtgtcggc gcggttcggc agcgacagtt
     3301 cccagcgcga gtcgtgggcg acgtcggtgg aattgtacag ccggtcgctc tccggcaaga
     3361 gcggtttttc accgcgaagg tttcgcagcg cccaaaactc ttgagtccag gcgacgtggc
     3421 cgatttccca aagcggcgga ttgacgatgg ccaagcgcgg gccgatcatt tgacgatcgt
     3481 cgagatcggc gaccaggctg agcaagcgat cgcggaactc gcgcatggtt gcgatcaatt
     3541 ggcgcgcgcc catggtggcg ctggggccga gggtggcgct agcgatcgtc tttgacattt
     3601 gggcagttgt cgaggcggac cgggaagatt cttgcattgc tctaacctat atctccgggc
     3661 atcacaaacc ggcaatcagt ttcgcgatgg ctgggcgcca tggcgctaat tcttgcgccc
     3721 agcgtcgacc gcgatttcgt tcgatgtagg cgaatgatag tcgcagacga cgcgtctcgg
     3781 cgggtgatat ttgcaacagg cgtctatcct ttttggcggt gatgctcgct tcggcatagc
     3841 tcctgagagc acgccaatgg ggtaacgcct cggcgccgaa gcgctggcga acgattctcg
     3901 tccaggtcaa gtcccctcgg tatttagccg gagcgcgcgc atagtcgaag catgttgcca
     3961 acggtatata gctcaactct tcctggagca atggattgtt aagatagcca tagacatgct
     4021 ccgccaaccg tgggtcgcgt cccgtcaggg gcgcgagatg caactcgtcg ctcatggaca
     4081 gatcgttaac cgggtaatta tcccatagca gcaggggacg ttcgatcatt ttcccgatcc
     4141 gctttacatg ggagagggcg atggtctttg aaaccaccga gggaccggtc cagaagcagg
     4201 ggacattggc cggcaggtag agtcccaaag tctcgagaaa gttcggctcg aatttgccga
     4261 aaacccgttc gagcagtgca tcttcgctgt aataggtcgg acaaatccac cactcgacgc
     4321 cgcgccaatg cgctggctgc gcggccatga tttgagccat ccaagttcct tcggcccgcg
     4381 ccagactatt cttgaaggcg cgtcggtcgc gcgcatcgct caagcgcgaa gggatatcat
     4441 cgaatagcac ggtgaaggtg cgcacgccga catcgtagaa gcgctgcgct ttgtgaagaa
     4501 gcaagcgcac catgtgatcg tcgcaaaagt acaaaccctc gccagggtga aaaccgtaga
     4561 cgaagtcaat ttgacttttt tgtgcggccc gaatcaatcg cgttaaggcg tgccattgtg
     4621 ccctgggata aggtcgtcgc caaagtttgc ggtgataggg gtcgtccttg ggcgcatata
     4681 gatacgtatt catgccgcgc gcggcgccaa atgcgaacag ccgccgccga tgccccatcg
     4741 accacatcgg gccgaagaat ccttcaacga tgccgcgtcg ctgaaacgtt ttggcggcac
     4801 tcatgccgcg ctaatttcgc acagcagcgc tggtgcaaca agattccggt catcagcgcg
     4861 gacaaaccaa atcagcggca tcctgtggcg acgaagaggc aacgtcccca atcctttgca
     4921 accgagtaaa tgagcgcgat tgtggagata aagagcaaga gccatgacga cttgcgttac
     4981 gcgatatttc cgacgctttc cgtgggtggc tataaattgg ccggtaggtg gaccgcaggt
     5041 tgtcgtcgaa aggtcatccg gagttaaaca gttttcgagc ggtacaacgg gagatgacat
     5101 ggaggggtgt aatttagacg acggcagtgc gaggcacata ccgccgtgct ttgctggtct
     5161 gctagggggc tcgcctagct cttcaaaaac ggcacgccta ggcgcatcag gtcgtcgaag
     5221 gcatcccaat gcaccatgtg cttgcagcca ttgacgatgt ggaggttgag atggggttgc
     5281 tgttttttca gcaattcggc gcgttcgccg gcgtgtgcac ggtcttcgcg gccaaatatc
     5341 atcaatagcg gcatctttag gtcggcgagc ctctcccata gcggtttggc cggcgtcgag
     5401 gccccggccg gcgccgcagg ggcctcgtga ttctgacggt cctggtgtgc tttgaaattg
     5461 cggccgatca tgctgcggtg acgcagggcg acgtcttcat cggagatcag cgaatggttg
     5521 aaggtatccg cctgcaggag tttgcgggca tcctccaccg tcggttcctt ttccgccatc
     5581 tctttgtcga cgcgagcctg taccgcttcg tacctgccga cctgtgcttc ggtctgcagc
     5641 ggcagcagcg agccggtgcc gagaatgatg acgtgcgaat agcggctggg atcttctaat
     5701 gccagctgaa ttgcgaatcc gcccgaacgc gaatgggcga tcagcgcggt cttgcccaag
     5761 ccagcggcat cgatgaattt tgggatggaa ttgcgttgct gcgccgtgct ttgcttggcg
     5821 ggaacgtcgg agagtccaaa accgggatag tcaaaggcga tcgcgcgaaa tcccgccttg
     5881 gcgaaagggc cgagattgcg ctgaaacacg tcagccgaac ttccgagcga ggcgccgtga
     5941 aggaataaca ccgatggtcc tgaacctgct tcgacataac gaagcttaag tccatccaca
     6001 gtaacgaacc tgtcttccgg tttgattttt tcagcagtag tcatgacatg gtctccaatt
     6061 acggcaaatg agattccaaa ccgccttttc tgacgggagc tatatcgccg aaccggcgag
     6121 gtgtcaatct tgcgagcgct cggcgcaggt taattttgtc ctttcgtaac gcagtacggc
     6181 gcgtagagat aagcatgcga atagggcagc cgccgccgat tgagtcatca acgaaaacga
     6241 cagaagaagc cttcgacaat aatgcggtgg tgaaatgtcg tcgcgtcatt ctgccgcgac
     6301 aatttcgcac agtattgcga attgaataac catagcatga acccgcggtt aactttaggt
     6361 caaacctaag tgcgtcgggc cggtggggtt ggatggggcg actacgcttg tgaaacccgc
     6421 cagagcgagc ggttggccga tatgcttttc gacgcttcgc tcggtatgtc gaggattcat
     6481 ttcatgattt gttttgcgaa ggaggcattt ggcgggcttg aagtctgcag cttttgctct
     6541 ggacggcagc catgagtgcc gagcaaaata gcagcgttat tacttttgca gtgttccgcg
     6601 ccttcgtttt tcgatatcaa tatcgacctg ccgtgatttt tcaatggtgt acttgagttt
     6661 gtccttttcc tgcaacaaag cctcccgttc ttttgtcaga cttcgcttct catcggcgat
     6721 tttttgatgt tcctccagga cagtaatcca ggtcttggcg ggatcaacca tcggactgtg
     6781 cggatagtcc tttacgagtg tacgaaacga cgaaagggcg cgaggatagt tttttttcgg
     6841 gttggtcgga tgggccgaaa tcattcccat attaaaaagc gccacgtcgg gggagccttt
     6901 gcggtcttga aaaatttttt gattttcaat aatcgcggct tcgtactgtc cctgagagaa
     6961 tagagccttg ctttgctcga agggatcggt ccgagtttgc accacagggg gaaccggtgg
     7021 caggggtgcg cttatcggtg acacgggcgc gctgttcatg gacgcgcagc cagacagcca
     7081 gactccccaa agcagtgtta tcaaccacca tgaactacat gacgctcggg tcttcatttc
     7141 gtcgcaccaa aacctttcag ccaatagtga aaacgcaaca atcgctttcg cgcgatcggg
     7201 cccgcgctcg gcgcctgttc cccgtcctga ggtattttgg cgccgagata gcacgcaaaa
     7261 aatatcccca taacgaccaa taccaaagtg gcagccaggg tgatcttttg gctcttcatt
     7321 gccgcatgtt caatgtattc gattctttcc tgcaagctct tctgcacatc gctcttaaaa
     7381 gcctcatttt ccctcagtaa ataatcgacg aggcgatcct tctcctcgcg atagcgggtt
     7441 tccgcatagg catgttttgt cttgatgtaa acgacttcct tttcaaacaa ttgacgatac
     7501 tgcccatgaa actcttcggc gcgggctagg cgctgaccca tgccccttga gccggccagt
     7561 ggttttagta gcgcagtgta ccggtcgaag tcggatccga actgcatata ttgctcataa
     7621 tgctgggtgg catgagtaac gttgaactgg cccgcatatc tgacctcgga aaggaaagca
     7681 tcggcaagtt tctccgagta atcgatcatg cgctggtcga ccttgagagc aatgttcgcc
     7741 gcattgctga gctttcccag ctgaattatc gaataacctg acactgtgac cattaggatc
     7801 agcatcaacg aatagccgat aatgcaacga tggagcgtgg atcgcgtaat cacaattcaa
     7861 cctccatggc tgggcgcggc gcaacgatag aggatccagt gccggcgaga cgatcgtcat
     7921 cgtgcacatc gctcgacaaa atgatgggca ggcgtacagt aaacgtcgta ccttcgccta
     7981 gcttgctgca aacgctaacc gaccccccca gtaggtcgac gaaattcttg actaggtaaa
     8041 gcccgagtcc ggcgccggaa taattgcgcg tcgtggcgtt gtcgatctga ttgaatttgt
     8101 cgaagatgtg cggcaaaaat tcctcggcca tgccaattcc cgtgtccgat acggagaact
     8161 cgataaatgg ctcgtcgggc acggagcgaa atgatatatc gacggaacct tgatcggtga
     8221 atttgatcgc gttgtttacc aggttgatcg ctacttggcg aagcttcatc cggtcggtta
     8281 cgaagtttgg cagatcggcc ggtattcgcc agttgatcgc cagatttttt tccttcgacg
     8341 tcatggcgta gtcggattcg agatcccgta ttagttcgtc aagattgatg gattccgcgt
     8401 tgatggccat gcggccggat tcgatcttgg tgatttccaa aatgctattg atcagcgaca
     8461 gcagatggct tgcttgcacg cgaatttttt ccgtgccttt ggcgtgttcc tgggtgatgt
     8521 cgccaaatat gcccatatta aacgcttcgg cgcagttcat aatgatattt agcggcgtcc
     8581 gtaactcgtg ggagatgact ccaagaaatt cgtctttaat ccgattcgac ttttccagtt
     8641 ccaccgcttg atttctggtc tgctcaaaca agtgtgaatt ataaagtgcg atggcggctt
     8701 ggtgcgtgag gtccgctaaa aaggcctctt ctatccgtaa atcgattgtt tccgtggagt
     8761 aaaagga
//
LOCUS       DASQKS010000540         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3248081, whole genome shotgun sequence.
ACCESSION   DASQKS010000540 DASQKS010000000
VERSION     DASQKS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248081"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VE170_13800"
     CDS             complement(<1..814)
                     /locus_tag="VE170_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ16564.1"
                     /translation="MYEKYFGFKTLPFRITPDPRFLYENPSCREAMASLCYGIEGRKG
                     FIVITGEPGTGKTRLVKDFMQRASVVIRTAFIPTPKLTSTELLGFVLNDLGIVTTTQD
                     RGVLTLQLKDYLIEQFEKRHIVALLVDEAQQLSNELLEELRLLSNLETNEDKLIQIVL
                     LGQPELETRLDQPDLRQLKQRVALHCSLAPLKDDEVNLYMLARLKTAGFEEKMLFEPK
                     AVEKIALYSKGIPRLINVICDNALITCYASSKKVVSADFVEEVARDLKLVDGP"
     gene            complement(1145..2149)
                     /locus_tag="VE170_13805"
     CDS             complement(1145..2149)
                     /locus_tag="VE170_13805"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HYJ16565.1"
                     /translation="MMHNVTLIPGDGIGPEVVKPAIAVVKAAGAKIDWDSHLAGAEAL
                     KKHGTTIPRTLIDSFNKTRLALKGPVTTPVGEGFSSVNVELRQTFDLYANLRPIKNLP
                     GVKARYQDVDLVVVRENTEGLYSGIEHEVVPGVMESLKIITDRASTRIAKFAFDYAKK
                     HGRKKVVAVHKANIMKLTDGLFLDCARKMAQKYPRIGFSDIIVDNACMQLVLDPLQFD
                     ILLMENLYGDIVSDLAAGLVGGLGLVAGANLGDRHALFEPVHGSAPDIKGQGIANPSA
                     MIIAAVMMLRHIGERTAARRIYTALETVLRRGDCVTRDLGGTATTKKFAEAIIKQIER
                     "
     gene            2326..3594
                     /locus_tag="VE170_13810"
     CDS             2326..3594
                     /locus_tag="VE170_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ16566.1"
                     /translation="MEDGNTPITTTGQAGETVDTVYRNQSRSVLATLIRLLGDFDLAE
                     EALHEAFKAALEQWPRDGVPANPRAWLVSTGRFKAIDAIRKRSRLEPLPDDLAGRLTT
                     DGPDADPLDRDSVEDDRLRLIFTCCHPALALDAQVALTLREVCGLTTDEIARAFLTAA
                     PTLAQRIVRAKAKIRNARIPYQVPSRADLPDRLDAVLRVVYLVFNEGYSASSGTEITR
                     HDLSSEAISLGRLLYDLLPEPDVAGLVGMMLLHDSRRAARASPAGDLVLLEDQDRALW
                     NRDYIKEGTVLVQRALAAGAVGPYTLQAAIAAVHANAAATEATNWGEIVALYDLLVRA
                     EPSPVIELNRAVAVAMRDGPETGLKLIDAILARGDLENYHLAHAARADLCRRLGQTAA
                     ARVSYQRALSLTQQAPEKRFLERRLEELKK"
BASE COUNT          886 a         1125 c         1044 g          852 t
ORIGIN      
        1 gcgggccgtc gacgagtttt aagtcgcgtg caacttcttc aacaaaatcg gctgagacta
       61 ctttcttgga actggcgtag caagtgatca gagcattgtc gcatatgaca ttgatgagcc
      121 gcgggatacc cttcgaatag agggcgattt tctccaccgc ttttggttca aacaacatct
      181 tctcttcgaa gccggcggtt tttaacctag ccagcatata tagattaact tcgtcgtctt
      241 taagcggggc gaggctgcaa tgcagcgcca cccgctgttt gagctggcgc aaatcgggtt
      301 ggtcaagcct tgtttccagt tcgggctgcc cgagcaagac aatctggatg agcttgtcct
      361 cgttggtttc cagattggaa agaagcctga gctcttcgag cagttcattg ctcagctgtt
      421 gagcttcgtc caccagcaga gcgacgatgt gacgcttctc gaactgttcg atcagatagt
      481 ctttgagctg aagtgtcagg actccccggt cttgagtggt agtaacgata ccgagatcgt
      541 ttagaacgaa accaagcaat tcggtcgatg tgagtttcgg cgtagggatg aaggcggtcc
      601 ggataaccac cgatgcccgt tgcatgaaat ccttgaccag ccgcgtcttt cccgtacccg
      661 gctcgccagt gattacgatg aaccccttac gcccttcgat gccgtagcac agacttgcca
      721 tggcctcgcg acaactcgga ttttcataga gaaatcgcgg atcgggtgtg attctaaagg
      781 gtagtgtctt gaagccgaaa tacttttcgt acatttgcga ttatccggcg attagaaaac
      841 taaatgccct gacttcgcca tcaccatcgt cccgagaata ggacaacccg aaattgttcc
      901 gtgtagatta ttagacgcaa ggtcgatgcc aatattacat atcaaagtgg ttgactgaaa
      961 caataggaaa agctctattc gaaacatttt ccatatgaag gaaatcccct gcgccatgcc
     1021 ggaaaaccag tgaatagtga gaggcaaaac cggattgctt aggaaataat tttaacccag
     1081 atcgcggctt aactttccca gccaagcata gcaagacccg gcgcatcatc aacaccgtta
     1141 gagtctatct ttcgatctgt ttgattatcg cctcggcaaa tttcttcgta gtcgccgtgc
     1201 cgccgagatc gcgtgtaaca caatcgccgc gccgcaggac tgtttccaac gccgtgtaaa
     1261 tccgcctggc cgcggtgcgc tcgccgatat ggcgcaacat catgaccgcc gcaataatca
     1321 tcgccgacgg attggcgata ccttggccct tgatatccgg cgcgctgcca tgcaccggct
     1381 cgaaaagagc atggcggtcg ccgagattgg cgccggcaac taatcccaac ccccctacca
     1441 atccggcagc caagtccgac acgatgtcgc cgtaaaggtt ttccatcagt aagatatcga
     1501 actggagcgg atcgagcacc aactgcatgc aggcattgtc gacgataatg tcgctgaagc
     1561 cgatgcgcgg gtatttctgc gccatcttgc gggcgcagtc gagaaacaga ccgtcggtga
     1621 gtttcataat attggcctta tgcacggcga cgactttttt tcggccatgc tttttggcgt
     1681 agtcgaaggc aaacttggct atgcgcgtcg acgccctgtc ggtgatgatc ttcaagcttt
     1741 ccatcacgcc cgggactact tcatgttcga tgccggaata aagcccttcg gtgttctcgc
     1801 gcacgaccac caagtcgaca tcctgataac gcgctttgac gccgggcaaa tttttgatcg
     1861 gccgcagatt agcatagaga tcgaaagtct ggcgcagctc gacattgacg ctgctaaatc
     1921 cttcacccac cggcgttgtc accggcccct tgagagcgag tcgggtctta ttgaacgaat
     1981 ctatcaatgt ccgcggaatc gtcgtcccat gtttctttaa cgcctcggcg ccggcaagat
     2041 gcgaatccca atcaattttc gcgcccgcag ctttgacgac tgcgattgcc ggcttgacca
     2101 cttcgggccc aatcccgtca cccggaatca acgtgacatt atgcattatg atttttgccc
     2161 tttcgtcggc cgaatcatat caggcggagc ccgctatatg taagccgaat ctcattaccg
     2221 ttcatgtatc aaacggcgcg ggatttttct tgggcaacgg cctaaacaca ggcttctttt
     2281 cgtcgtacac ttgatccaac gcaaacggat tggagcaggc gcaacgtgga agacggcaac
     2341 accccaatca cgacaaccgg gcaggcgggc gaaacggtgg acaccgtcta ccgtaaccaa
     2401 tcacgctcgg tcctggcgac gttgattcgc ctgcttggcg acttcgatct cgccgaggag
     2461 gcgctgcacg aagccttcaa ggccgcgttg gaacaatggc cgcgcgatgg cgtgccggcc
     2521 aatccgcgcg cctggctggt ctccaccgga cgattcaaag ccatcgacgc gatccgcaag
     2581 cgctcgcgat tggaacccct gccggatgat ctcgccgggc gcctgaccac cgacggcccg
     2641 gacgccgatc ccctcgaccg cgacagcgtc gaagacgatc gcttgcgctt gatctttaca
     2701 tgctgccatc cggcgctggc gctcgatgcc caggtcgccc tcacgttgcg cgaagtgtgc
     2761 ggcctcacca ccgacgagat cgcccgtgcc ttcttgactg ccgcgccgac attggcccag
     2821 cgcattgtcc gcgccaaggc gaagatccgc aacgcgcgca tcccctatca agtgccgtcg
     2881 cgcgccgacc tacccgatcg cttggacgcc gtgttgcgcg tcgtctattt ggtattcaac
     2941 gagggctact cggcatcctc ggggacagaa atcacccgtc acgatctatc gagcgaggcg
     3001 atcagcctcg gccggctgct gtacgatttg ttaccggagc cggatgtggc agggctggtg
     3061 gggatgatgc tgttacatga ttcgcggcgc gcggcgcgcg cctctcccgc tggtgacctg
     3121 gtgctgttgg aagatcagga ccgcgcgctg tggaaccggg attacatcaa ggaaggaacc
     3181 gtgctggtcc aacgtgcctt ggccgccggc gcagtcggtc cttacaccct tcaggcagcg
     3241 atcgccgccg tacacgccaa tgcagctgcc acggaagcta caaattgggg cgagatcgtc
     3301 gccctgtacg atctcttggt gcgtgccgag ccgtccccgg taattgaatt gaatcgcgca
     3361 gtggcagtgg ccatgcgcga tggtcccgag accggtctca agcttatcga cgcaattttg
     3421 gcgcggggcg acctggagaa ttatcatctc gcccatgccg cgagggcgga tctctgccgc
     3481 cgcctgggcc aaaccgccgc agcccgagta tcctatcaac gagccctgag cctcacccaa
     3541 caagcgccgg aaaagcggtt tctcgaacgc cgactcgagg aactaaaaaa ataaccagcc
     3601 gattgtcgat ttcgctctag accggacgac tagttggtat caggggagaa aaaagtatgg
     3661 ccagcacaga caattcagcc gcgccagccg gtcatagtct gcgaagcttt gggcggcgac
     3721 aggatttttc gaagtgaatc agatgagagc caaacaaaaa gccgccaaaa acatcgatgc
     3781 gtatatcgcg agatttccag acgaagttca aaagattcta aaaaagataa gaatgaccat
     3841 tagacaagcc gcaccgcagg cacaggaaac cattagctat cagatcccgg ccttcatttt
     3901 aaaaggt
//
LOCUS       DASQKS010000541         7363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4905330, whole genome shotgun sequence.
ACCESSION   DASQKS010000541 DASQKS010000000
VERSION     DASQKS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4905330"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VE170_13815"
     CDS             <1..225
                     /locus_tag="VE170_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16567.1"
                     /translation="YYAGDIGDRTFASTGGMTEPVLQALGIRYYVMRDIDDAPELIKR
                     AQILAEDGKKPIALLLTKEVLGRRAALKSL"
     gene            251..856
                     /locus_tag="VE170_13820"
     CDS             251..856
                     /locus_tag="VE170_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ16568.1"
                     /translation="MKRYDCLQALVAEIKDALVVSSAGAMTLEWNALHPSDGNFRVRT
                     LGLCSSIALGMALGLPHRKVVALDGDGSLLMNLCSLPTIARMRPSNLVHIVFDNEVYE
                     ASGSKKTATGAGTDLVGMARAAGIKNSHWSKTVEEFAAAAATALRGTELCFIGAKVTT
                     ERTAVPPFPIDEVENKYRFMRHIEQSEKIEIFKVNLPASYT"
     gene            876..1892
                     /locus_tag="VE170_13825"
     CDS             876..1892
                     /locus_tag="VE170_13825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16569.1"
                     /translation="MKIDIEFNSGAQLPMDAIPELARQAEMHGFDCAWGGEANNKDPT
                     VMLSAIGAATRQIKVGSAIYHILGRTPATLALQAMGLDELTGGRFLLGIGSSNPTIAK
                     WHGQTFDHPLGRVREYIEITQAAMRGEKLAFDGKYFTAHNFKMAFKPSGRTIPIYLAA
                     FGSQMTRLAGRISDGVLINMANPSEIQRIADEVRAGALEAGKDPAQMEIICKIRCSIA
                     PNYDTAREALSHALTYYALADYYRDLLARMGFADEVEAMRGAWKAGGFHAARKLISVR
                     MFANLPLVAATSAPQVVQQIKPYVEAGATRVILAYVAASDDMVGEMQSFIDSWSPAVT
                     NSFG"
     gene            1917..2318
                     /locus_tag="VE170_13830"
     CDS             1917..2318
                     /locus_tag="VE170_13830"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ16570.1"
                     /translation="MPFDLINPAGLESPVGYAHVARITGGTVIHIAGQAPFDVNGQVV
                     GKGDFVAQFTQVMSNLRTAIESVGGKPNQYAVLTIYVTQLEDYWGNTKPLGTAYRGVF
                     GKYFPAITLVEVKRLYNPDCMLEISGMAVID"
     gene            2484..2693
                     /locus_tag="VE170_13835"
     CDS             2484..2693
                     /locus_tag="VE170_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16571.1"
                     /translation="MMRPNEISSLNRERDPDLFDTVTEEEIIYLWRSIKDRGLSEDEA
                     WQAVVTGMALDMAFAKSKSCALTEH"
     gene            2854..3048
                     /gene="rpoZ"
                     /locus_tag="VE170_13840"
     CDS             2854..3048
                     /gene="rpoZ"
                     /locus_tag="VE170_13840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYJ16572.1"
                     /translation="MSRITVEDCLDKVANRFELVLLASKRARHLLKGAKPLIDSENRE
                     VVVALREIAAGKVRKVPENP"
     gene            3176..4132
                     /locus_tag="VE170_13845"
     CDS             3176..4132
                     /locus_tag="VE170_13845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HYJ16573.1"
                     /translation="MAEEDKTPATELSFSEFDLPKVVEEEEPGSSRKALVPFDPLQRY
                     LVEIRRFAVLSREEEHKLAVEFKEFGNIEAAYKLVTANLRLVVMIAREYQKSFKTLLD
                     LVQEGNMGLMEAVKNFDPYRGVRFPSYAVWWIRAYMIRYIMNDWRMVKIGTTQAQRKL
                     FFNLQKEKARIEAEGLTVGPKLIAERLNVKEDEVVEMEQRLASRDLSVDMPVGDDGEA
                     TLLHFLPDSKQNPEEEYAESQYQQLLREKMEQFAVGLKDKELVIYRERLLNEDPLTLR
                     EIGDKYGISRERVRQIEERVKKKLKTYLSKEFKDANNIGGAS"
     gene            4253..4705
                     /locus_tag="VE170_13850"
     CDS             4253..4705
                     /locus_tag="VE170_13850"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ16574.1"
                     /translation="MQFEKQIEIAAPREKVWKFIWDIDRFISCVPGCKDAKTVEEGKR
                     YSATMVERVGPFKVEFPTTIEVLEREELTHIKAQASGADNKIGSRMKIDLDVNLREEG
                     NKTVVSFIAGVDILGKLAALGHGIIKRKADQVLDEFAHAVKTTLEGDK"
     gene            4725..5558
                     /locus_tag="VE170_13855"
     CDS             4725..5558
                     /locus_tag="VE170_13855"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYJ16575.1"
                     /translation="MEEPESVAEVSELLGRFGDSAKIYAGGTELLLAMKEGLIHYERL
                     INVKQLKDLGAVTEDNGSIKIGALCTHRQLETSTLLQEKLPSLVKLEQNVANVRVRQA
                     GSIGGNLCFAEPHADPGTLLMALGAKMIAEKNSGKREIEAQDFFVDAYETSLQADELL
                     TEIRVPKPGGNGRSAYLKFGYLERPSVGVAVALTLDGTTVNDVRIAVGCAGPAPKRVA
                     EAEAILTGKGKDEAVALLQQAGAVAGRASEAISDLHGAQDYKEHIVGVLLKRAFNSIV
                     G"
     gene            5596..6108
                     /locus_tag="VE170_13860"
     CDS             5596..6108
                     /locus_tag="VE170_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ16576.1"
                     /translation="MSRSMTRPAQGQTIPMSFTLNGRVQEIDVEPNELLLDVVRDRLG
                     LTGAKRSCDVQVCGACTLLVDGRPVSACTTLAFEVRGRSVMTIEGLADDGKLHPLQEA
                     FIEHGGFQCGFCTPGMLLASKALLDENPNPTEEELKHFMHGNLCRCTGYKKIIESIMA
                     AAQKMRASRA"
     gene            complement(6203..>7363)
                     /locus_tag="VE170_13865"
     CDS             complement(6203..>7363)
                     /locus_tag="VE170_13865"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16577.1"
                     /translation="KWDHALGNPIPPRMVSAKNAPCKENILHGKDVDITKLPVPIWTV
                     GEDPAPFFTSPYLITKDPETGVRNVGTYRMQVKGPNKTGLLIGKRQDMAWHMYKNNDK
                     NQPTPVAVVIGADPSIGYVSVSKMSDALDEFAVAGALRGAAVDLVPCETVPLEVPATA
                     EIVLEGEIPPNALEHEGPFGEYTGYMGPAGNEPYFNIKCMTYRNQPLYQAFISQMPPS
                     ESSCIRGIGREWPLFKHLKDTLRIPIKDLRLKEAGGSGAYLVISLKKEFAGQVRQLMY
                     GAWSQRTGFGKITVIVDDDIDVWDDFAVDWAISWHVRPDKDVYIERDVQAVGLDPSQA
                     PADVLQHHPSRYLGSRMAIDATRKHKYPAISLPPKEHLDLVASRWREYGIED"
BASE COUNT         1663 a         1956 c         2123 g         1621 t
ORIGIN      
        1 tattacgccg gcgatatcgg cgaccggact tttgccagca ccggggggat gaccgaaccg
       61 gtgctgcagg cgctgggcat tcgttattat gtcatgcgcg atattgacga tgcgcccgag
      121 ttgatcaaac gcgcgcaaat cctcgccgag gacggtaaaa aaccgatcgc tcttttactc
      181 accaaagaag tcctgggacg gcgcgcggcg ttaaagtccc tgtaaagagt tgtcacaagt
      241 ggtcacaagt atgaaacgat acgactgttt acaggcactc gtggccgaga taaaggatgc
      301 cctggtagtg agttccgcgg gtgccatgac gttggagtgg aacgcgttgc atccaagcga
      361 tggcaacttt cgcgtgcgta ccttggggtt gtgctcgtcg atcgctctcg gtatggcgtt
      421 ggggctaccc catcgcaagg tggtcgcgct cgacggcgat ggctcgttgt tgatgaatct
      481 ttgctcgctg ccgacgatcg cccggatgcg cccgtctaat cttgttcaca ttgtcttcga
      541 caacgaagtc tacgaagcat cgggcagcaa gaaaaccgca accggtgcgg gtacggatct
      601 cgtcggtatg gcgcgcgccg cggggatcaa gaattctcac tggtcgaaaa cggttgagga
      661 attcgccgcg gcggcggcga cggcgttgcg tgggaccgaa ctatgcttca tcggcgccaa
      721 agtcaccacc gaaagaaccg cggtaccgcc gtttcctatc gatgaggtcg agaataagta
      781 ccgcttcatg cgtcacatcg agcaatcgga gaagatcgag atcttcaagg tcaatctgcc
      841 ggcgagctac acctaattgg ttttttcatc gcatcatgaa gatcgatatt gaattcaatt
      901 ccggcgccca attgccgatg gacgccatcc ccgaattggc gcgccaagcg gaaatgcatg
      961 gctttgactg tgcctggggt ggggaagcga ataacaagga tccgacggta atgctgtccg
     1021 cgatcggcgc ggcaacccgg cagatcaagg tcggctcggc gatctatcat attctcggcc
     1081 gcacgccggc gaccttggct ttgcaggcga tggggctcga cgaactgacc ggcgggcgat
     1141 ttttactggg catcggctcg tccaatccga ccattgccaa atggcatggc cagaccttcg
     1201 atcatccgct tgggcgcgtg cgcgagtaca tcgaaattac ccaggcagcg atgcgcggcg
     1261 aaaaactcgc tttcgacggc aagtatttta cggcgcataa cttcaagatg gcgttcaaac
     1321 cttccgggcg aacaattcca atttacctgg cggccttcgg ctcgcagatg acccggctcg
     1381 ctgggcgcat tagcgacggg gtgttgatca atatggcaaa cccgtcggag atccagcgca
     1441 tcgccgatga ggtgcgggcg ggagcgctgg aagccggcaa agatccggcg caaatggaaa
     1501 ttatctgcaa gattcgctgc tcgatcgcac ccaactatga cactgcccgc gaagcgctca
     1561 gccatgcgct cacctactat gcacttgccg attattatcg tgatcttctc gccaggatgg
     1621 gtttcgccga cgaagttgaa gccatgcgcg gcgcctggaa ggccggcgga tttcacgccg
     1681 cccgtaagct gatttccgtg cgcatgtttg cgaacttgcc gctggtcgcc gcgacttcgg
     1741 cgccgcaggt ggtgcagcag atcaagccct atgtcgaagc cggtgcgacc cgcgtgatct
     1801 tggcctatgt cgcggcgtcc gatgacatgg tcggcgagat gcagagtttt atcgattcct
     1861 ggagtcccgc cgtgacaaat tcttttggct agagcaaagg aaaaaaggag tgaatcatgc
     1921 cgttcgatct catcaatccc gccggattgg aatcgccagt cggatacgcc cacgtcgcta
     1981 gaatcaccgg cggcaccgtg atccatattg ccggccaagc gccgttcgac gtcaatggcc
     2041 aggtggtcgg gaagggcgat ttcgtcgccc aattcaccca ggtcatgagt aatcttagga
     2101 ccgcgatcga gtctgtcggc ggtaaaccga atcagtacgc cgtgctgact atctatgtaa
     2161 ctcagttgga agattattgg ggcaatacca agcccttagg caccgcctac cgcggggttt
     2221 ttggcaaata cttcccggcc attacgttag tcgaggtcaa gcgtttatat aatcccgatt
     2281 gtatgttgga gatttccgga atggcggtga tcgattaatc ttgggcgccg gaattttcgc
     2341 ctgtgaggcc aaatttgcgc attctccact catcccaact agtccattag atgtgaaacc
     2401 acgcagtttc aagtggttac taacctggcc caccaattgc tcattgattg accatcatct
     2461 gccaaaggcg gtccaatact atcatgatgc gacccaatga aatttcctca ctcaatcgtg
     2521 aacgcgatcc tgatctattc gacacggtta ccgaggaaga gatcatctat ctctggcggt
     2581 cgatcaagga ccggggcctt tccgaggacg aggcgtggca ggcggtggtg accggcatgg
     2641 ccctcgacat ggcttttgcc aagagtaaga gttgcgccct gaccgagcac taaaacgctc
     2701 tcgttgccaa ttctctagat caattttctc cggcccaaag tatagcgatt cttccttcca
     2761 tgctgcccct gcgcccttcg caaagcggcg ctaattccat tgatttacgt ggtgaaatct
     2821 gtttgaatct cgcagatagt tgaggagatt ctaatgtcac ggatcaccgt agaagactgt
     2881 ttggataagg ttgcgaatcg tttcgagttg gtgttgttgg cgtcgaagcg agctcgccat
     2941 ttattgaagg gtgccaagcc gctgatcgat tccgagaatc gcgaagtcgt cgtcgcactg
     3001 cgtgaaatcg ctgccggcaa agtgcgcaag gtgccggaaa acccctagtt cattccaatc
     3061 ccacttaaac gcagcggtca agccggcctc aagatttatt ttaaaataga tcacatcgag
     3121 gtcctattga gccactagat cgcggtcttt ggtggtgagg ttgttgacac cgcgaatggc
     3181 cgaagaagat aaaacacccg caactgagct ttccttctcc gaattcgatc tacctaaagt
     3241 cgttgaagag gaagaaccgg gcagcagccg caaggcgctg gtgccgttcg atccgctgca
     3301 acggtatctc gtcgagattc gccgttttgc ggtcctcagc cgtgaggagg agcacaagct
     3361 tgccgtcgaa ttcaaagagt tcggtaatat tgaggcagcc tacaaacttg tcacggccaa
     3421 cctgcgcctg gtcgtgatga tcgcccgaga atatcaaaaa tccttcaaga cgctactcga
     3481 cttggtgcag gaaggtaata tggggctcat ggaagcggtg aagaacttcg atccttaccg
     3541 cggcgtgcgt ttcccgtcct atgcggtttg gtggattcgt gcctacatga tccgctacat
     3601 catgaacgat tggcgcatgg tgaagatcgg caccacccaa gcccagcgca agctattttt
     3661 caatctgcag aaggaaaaag cacgcattga agccgagggg ttgacggtcg gaccgaagct
     3721 tattgccgaa cgcttgaacg tcaaggaaga cgaagttgtc gaaatggagc agcgcttggc
     3781 cagccgcgac ctgtcggtgg acatgcccgt gggggatgat ggcgaggcca cgctgctcca
     3841 cttcttaccc gatagcaagc agaatcccga agaggaatac gccgagagcc agtaccagca
     3901 gctgctgcgg gaaaagatgg aacagttcgc cgtgggcttg aaggacaagg aattggtgat
     3961 ttaccgcgag cgtctgctca acgaagaccc gcttaccctg cgtgaaattg gcgataaata
     4021 cggtatcagc cgtgagcgcg tgcgccaaat cgaagaacgc gtcaaaaaga agttgaagac
     4081 ttatctcagc aaggaattca aagacgccaa taatatcggc ggcgctagtt gacggcggca
     4141 aagttgccgc gccatctcaa accctcgttc gtgtaagcaa attacttcgc gtcgcttgcg
     4201 tgaatcggcg ccatttgata gcaaaattag attgccagtc ggaggaacgt ctttgcaatt
     4261 tgaaaaacaa atagaaatcg ccgccccccg cgaaaaggtg tggaagttta tctgggatat
     4321 tgaccgcttt atttcctgcg tgccgggatg caaggatgcc aaaaccgtcg aagaagggaa
     4381 gcgctactcg gcgacgatgg tcgagcgggt gggtcctttc aaagtcgagt ttccgacgac
     4441 catcgaagtc ttggagcgcg aagaactgac ccacataaaa gctcaggcat cgggtgccga
     4501 caataagatc ggcagccgca tgaagatcga tcttgacgtc aacctgcgcg aagaaggtaa
     4561 caagaccgtg gtgagtttta tcgccggcgt cgatatctta ggcaagctcg cggccctcgg
     4621 ccatggcatt atcaaacgca aggccgatca agtcctcgac gagttcgccc atgcggtcaa
     4681 aacaacctta gagggagata agtgacatgc tcagaaggtt tcgattggaa gaacctgaaa
     4741 gtgtcgcgga ggtctccgaa ctgcttggcc gctttggcga cagcgcgaag atttacgccg
     4801 gcggcaccga gctcttattg gcgatgaagg aagggttgat tcactacgaa cggttaatca
     4861 acgtcaagca gctcaaggat ctcggcgcag tgaccgagga taacggttcg atcaagatcg
     4921 gcgccctatg cacccaccga cagctggaaa cctctacgct gttgcaagaa aaattgccgt
     4981 cgctggtcaa gcttgaacag aacgtcgcca atgtgcgcgt gcgacaagcc ggctccattg
     5041 gcggcaatct ttgctttgcc gaaccccatg ccgatccggg gaccctgttg atggcgctag
     5101 gcgctaaaat gattgccgag aagaactccg gcaaacgcga gatcgaagcc caggattttt
     5161 tcgtcgacgc ctatgaaacc tcgctgcagg ccgatgagct attaaccgaa attcgcgtgc
     5221 ctaagccggg gggtaatggc cgtagtgcct atcttaaatt cggctatttg gaacggccca
     5281 gcgtcggtgt tgccgtggcc ttgactctcg atggcacgac ggtgaacgac gtcagaatcg
     5341 ccgtcggctg cgccggtccg gcgcccaagc gcgtcgccga agctgaagca atactgaccg
     5401 gcaagggcaa agatgaggcg gtggcattgt tgcagcaggc tggggcggtc gctggccgtg
     5461 cctcagaggc gatcagcgat ctgcacgggg cacaagatta taaagagcat atcgtcggcg
     5521 tattgttgaa gcgcgcgttt aacagcatcg tcggctgatc cgcgcttata accgaacgat
     5581 gccaggagaa ctttaatgtc cagatcgatg acccgacccg cccaggggca aaccatcccg
     5641 atgtcattta cactcaatgg cagggtgcag gaaatagatg tcgagcccaa tgaattgctg
     5701 ctcgatgtcg tgcgcgatcg tctaggtctg accggtgcca aacgctcctg cgacgtgcag
     5761 gtgtgcggtg cctgtacttt gttggttgac ggccgcccgg tgagcgcttg cacgactttg
     5821 gcctttgaag tacgcggccg ttcggtcatg acgattgagg gtctcgccga tgacggcaag
     5881 ctccatccgc tccaggaagc gtttattgag catgggggct ttcaatgcgg cttttgcacg
     5941 ccgggaatgc tgttagcatc gaaggcgctt ctggacgaaa accccaaccc gactgaagaa
     6001 gagctgaaac acttcatgca cggcaacctc tgccgctgca ccggctataa gaagattatc
     6061 gaatcgatca tggccgcggc acaaaagatg cgcgcgagcc gcgcttaaga gttcaaccgt
     6121 tccaatcgtt caagcccttt ttcggatgcc cgaatagagt cggcgtcttg aactttttga
     6181 actacttgaa cattggaatc tgctagtctt cgatcccata ttctcgccag cggctggcga
     6241 ctaaatccaa atgctccttc ggcggtagcg atatcgccgg atatttgtgt ttgcgcgtgg
     6301 catcgatcgc catgcgcgag ccgaggtagc ggctcgggtg atgttgtagg acatcggcag
     6361 gagcctggga cggatcgagg ccgacagcct ggacatcccg ttcgatgtag acatctttgt
     6421 ccggccggac atgccagctt atcgcccaat cgacggcgaa atcatcccac acgtcgatat
     6481 cgtcgtctac gatcaccgtg atcttgccga atccggtgcg ttgtgaccat gcgccgtaca
     6541 tgagctgacg cacctggccg gcgaactctt tcttgagcga aatgacgaga taggcgccgc
     6601 tgccgccggc ttctttaagg cgcaaatctt tgatgggaat tcgcagcgtg tctttgagat
     6661 gcttgaacaa tggccactcg cggccgatac cgcggatgca actcgattcc gacggcggca
     6721 tctggctgat gaacgcctga tagagcggtt ggttgcggta ggtcatacat ttgatgttga
     6781 agtacggttc attaccggct ggtcccatgt agcccgtgta ttcgccaaac ggtccttcgt
     6841 gttcgagagc attgggcggg atctcgcctt ccaaaacgat ttctgccgtg gccggaactt
     6901 cgaggggcac ggtctcgcag gggaccaggt cgacggcagc gccgcgcagc gcgccggcga
     6961 cggcgaattc atccaatgcg tcggacatct tggaaaccga aacatagccg atcgatgggt
     7021 cggcaccgat gacgacggcc accggagtcg gttggttttt gtcattgttc ttgtacatat
     7081 gccaagccat atcctggcgc ttgccgatca gcagtccggt cttgttcggc cctttgactt
     7141 gcatacgata ggtgccgacg ttacgcacgc cggtttcggg atccttggtg atcagatagg
     7201 gcgaagtgaa aaacggcgcg ggatcttcgc cgacggtcca tatcggcacc ggcagtttgg
     7261 tgatgtcgac atctttgccg tgcaggatgt tttctttgca gggcgcattc ttggctgaaa
     7321 ccatgcgcgg tggaatagga ttgcccaggg catggtccca ttt
//
LOCUS       DASQKS010000542         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_722693, whole genome shotgun sequence.
ACCESSION   DASQKS010000542 DASQKS010000000
VERSION     DASQKS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722693"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VE170_13870"
     CDS             complement(<1..395)
                     /locus_tag="VE170_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16578.1"
                     /translation="MASAIEKKPNPEEKYMSQTILDADSHVSEPLNLWQERLPEKYRS
                     IAPHMETQYQGQPGAWWHIEPDRQPHNVILGFGANKSMEELQKLLESFSYAGAHRGGW
                     DPAQRLQDMDQDGIAGDVLYTTLGFRMFW"
     gene            complement(408..1379)
                     /locus_tag="VE170_13875"
     CDS             complement(408..1379)
                     /locus_tag="VE170_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16579.1"
                     /translation="MQLKLTCRRQILTALILITGLIHAVPAAAQKLTQIHIGVPTNTV
                     TWFPLYVAWKKGIFQEQGIELLPVTISVRASLGALASRHLSYITPLGSSMTAIAKGLP
                     AKVIMIFAVRSHHVLVAKREIAAPGALRGRGVAISQPGGTVHRQFLKILEHYRVDAKS
                     VNMINLGEMPARALALKAGTIDAAMLSVPYDLFMEKDGFRPIAYMKDVAEFPLLGLIA
                     HDERLRERPDEVKKVLTAALKGIAYTKSHREELLPLLKQFVGLPDMEMTGKTFDVIKD
                     LWPDTGIPSDKGLTTALTMADVEADVPTARIVDWSFVSAASLSLKNR"
     gene            complement(1561..2358)
                     /locus_tag="VE170_13880"
     CDS             complement(1561..2358)
                     /locus_tag="VE170_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16580.1"
                     /translation="MFAQIKHAAIYTHNPQSAIAFYEKILGMKRITSAVTENNRGHIS
                     DGLIGLAVLARRPGIPAGLDHFGFEVDDVDSARQRIKKHYPHAIITKGLEKVPFALFR
                     SHDPAGAQFDISERTDPKVREGYKESGWDQARQFNHVVIRAHEPAAVADFYQEVFELF
                     EVREPPLGPAVCLSDGKVRLLIQPTNDNSYMSMRQGLDHIGYQVESIEQTKKDLAALA
                     VSAPASAPRDIAGGIFGHLTKRDMDGCKIGQYATSDPDGVVLDFSEA"
BASE COUNT          488 a          691 c          724 g          592 t
ORIGIN      
        1 atccagaaca tgcgaaaacc cagcgtcgtg tagagtacgt cgccggcgat gccgtcctgg
       61 tccatgtcct gcagtcgctg cgccgggtcc cagccgccgc gatgggcgcc ggcgtaggag
      121 aagctttcta atagcttctg cagttcttcc atcgatttgt tagcgccgaa acccaagatt
      181 acattgtgcg gttggcggtc cggttcgata tgccaccatg caccgggctg gccctgatat
      241 tgggtctcca tatgcggtgc gatggagcgg tatttttccg gcaagcgctc ttgccacaga
      301 ttcaacggtt cgctgacatg ggaatcggca tctaggattg tttgcgacat gtatttttcc
      361 tctggattag gctttttctc gatagcgcta gccataaata tttcattcta acgatttttc
      421 aaggagagcg aggctgcgct gacaaacgac caatcgacga tcctggcggt tggaacatcc
      481 gcctcaacgt cggccatcgt cagagccgtg gtcagtccct tgtccgaagg aatgccggta
      541 tccggccaaa gatctttgat cacgtcaaag gtttttccgg tcatctccat gtccggcagg
      601 ccgacgaact gtttgagcag gggcaggagc tcctcacgat ggctcttggt ataagcgatg
      661 cctttgagcg cggccgtcag gactttttta acttcgtcgg gtcgttcgcg caagcgttcg
      721 tcatgggcga tcaggccgag caggggaaac tcggctacgt ctttcatgta agcgatcggg
      781 cgaaagccgt ctttttccat gaataaatca tagggcacgc tcaacatcgc cgcgtcgatg
      841 gtaccggctt tgagcgcgag agcacgggcc ggcatttctc ccaggttgat catattgacg
      901 cttttggcgt caactctgta gtgctcgaga attttcagaa actgccggtg tacggtgccg
      961 cccggctggc tgatggcgac gccgcggccg cgcagcgcgc cgggcgcagc aatctcgcgc
     1021 ttggcgacca acacatgatg cgagcgcacc gcgaagatca tgatcacttt ggctggcaga
     1081 cccttggcga tcgccgtcat cgacgagccg agcggcgtga tgtaggagag atggcgcgac
     1141 gccagcgcgc cgagtgaggc gcgcaccgaa atggtcacgg gcaacagctc gatgccctgc
     1201 tcctgaaaga tgcctttttt ccaagcgacg tagagcggaa accaggtgac ggtgttggtc
     1261 ggcacgccga tgtgaatctg cgtcaacttt tgcgccgccg ccggcaccgc gtgaatcaac
     1321 ccggtgatga gaatcagcgc ggtaagtatt tggcgtctac aggttagctt aagttgcaaa
     1381 ttgcctctct atgataaatc ccgctgtcgc aatcctattg tgcaaccgct gcgcaaaacc
     1441 cggcggttgc aaaactctct gttggaattc ctctcatctc cctttaccaa gaaaggtgct
     1501 cggagacgac cgtgatcgtt gttcttcgca atcttgaacc agcgtccgca tcgactgtga
     1561 tcaagcctcg gaaaagtcca gcacgacacc gtcggggtcc gaggtcgcat actgaccgat
     1621 cttgcagccg tccatatcgc gtttggtgag gtggccgaag atgccgccgg cgatgtccct
     1681 tggcgctgac gccggcgctg aaaccgccag cgctgcgaga tcctttttcg tctgttctat
     1741 gctctcgacc tggtaaccga tgtgatcgag cccttggcgc atgctcatgt aagagttgtc
     1801 gttggtcggc tggatcaaca gacgcacctt gccgtcgctc agacaaaccg ctggtcccaa
     1861 gggtggttct ctgacctcga agagttcgaa gacttcctgg taaaaatcgg ccaccgccgc
     1921 cggttcgtgg gcgcggatga cgacatggtt gaactgccgc gcttggtccc agcctgattc
     1981 tttgtagcct tctcgaacct tggggtcggt gcgctcggag atatcgaatt gggcgccggc
     2041 tggatcatgg ctgcgaaaca aggcgaaggg gactttttcc agccctttgg taatgatcgc
     2101 gtggggataa tgcttcttga tgcgctgccg ggcgctgtcg acatcgtcga cttcgaagcc
     2161 gaaatggtcc aaccccgcgg gaataccagg ccgccgggct aacacggcga gaccgatcaa
     2221 accgtcgctg atgtgaccgc gattgttttc cgtcaccgcg ctggtgatcc gtttcatgcc
     2281 gaggattttc tcataaaacg ctatcgcgct ctggggattg tgggtgtaaa tagcggcgtg
     2341 tttaatctgg gcaaacatag tggactccgt ttcttaccgg ccgtaaaagc gccgggcgtt
     2401 gtcgccgagg attgcggtct tgtgctcttt cttgagatcg tcgcgttcca acacttcgtc
     2461 aatttcttcc atgcagttac tcatggagat ctcgt
//
LOCUS       DASQKS010000543         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1550308, whole genome shotgun sequence.
ACCESSION   DASQKS010000543 DASQKS010000000
VERSION     DASQKS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550308"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VE170_13885"
     CDS             <1..339
                     /locus_tag="VE170_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16581.1"
                     /translation="SGGIAYVLDEEGTLANRCNLETVSLETLDQRDMQEVEELLKRHA
                     VYTHSARAWQFLALWQETAPKFVKVMPKDYRRVVEALQAAEARGLVGDDALMAAFDAN
                     KNDASRVSGN"
     gene            382..1866
                     /locus_tag="VE170_13890"
     CDS             382..1866
                     /locus_tag="VE170_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HYJ16582.1"
                     /translation="MGKPTGFMEIARELPQDRSPLERVKDWQEFHEHFAETKLQQQGA
                     RCMDCGIPFCHTGQLISGMASGCPVNNLIPEWNDLIYRGLWQEALHRLHKTNNFPEFT
                     GRVCPAPCEGSCVLGLNELPVTIKNIEAAIIDNGFLQGWVTAEPPAKRTGKKVAVIGS
                     GPSGLACAAQLNRAGHQVTVYERADRIGGLLMYGIPNMKLDKKIVQRRIDLMAAEGVK
                     FVVNADIGAGMSARKIKDENDAVVICTGATKARDLPIPGRELKGVYMAMEFLRANTKS
                     LLDSQHKDGNYISAKDKHVIVVGGGDTGTDCVGTSMRHGCKSLTQLEILPRPADSRQA
                     DNPWPEWPKVYKLDYGQEEVAARFGADPRVYLTTGEKFAGDDKGNVKEMHIYDVEWTK
                     NEQGAFIPKRVPGSDKVLKADLVLLAMGFLGPEEPVLTELSVERDPRSNAKAEHGQFT
                     TNVAGVFAAGDCRRGQSLVVWAINEGRGAARECDRFLMGFSNLP"
     regulatory      2161..2251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2298..>2610
                     /locus_tag="VE170_13895"
     CDS             2298..>2610
                     /locus_tag="VE170_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16583.1"
                     /translation="MESPSEQKFIDQQKFSLGSADASSGAVQYLSYDRILRSLGSMLE
                     QKSLTEFELQLSDDVYTVRGRPSRRLSRGSSLLGRIFGSGGTLRNSRQPAMIEQQYPV
                     DD"
BASE COUNT          588 a          708 c          793 g          521 t
ORIGIN      
        1 tccggcggca tagcctatgt tctcgatgaa gaaggcaccc tggcaaaccg ctgtaacctg
       61 gagacggtaa gcttggaaac cctcgaccag cgggacatgc aagaagtgga agagttgctc
      121 aagcgccacg ccgtctacac ccatagcgcg cgcgcctggc agttccttgc gctgtggcag
      181 gagacagcgc ccaagtttgt caaagtcatg ccgaaggatt accggcgtgt ggtcgaggcg
      241 ctgcaagcgg ccgaggcgcg cggactagtg ggcgacgacg cactgatggc ggcgttcgac
      301 gccaataaaa acgatgcatc gcgggtgagc ggcaactgac caatgatatt cgtattgttg
      361 cgggaaagag aagattgagc gatgggtaaa ccgaccggat tcatggagat cgcgcgcgaa
      421 ctgccgcagg atcgttcgcc gctggagcgg gtcaaagact ggcaggagtt tcacgagcat
      481 tttgccgaaa ccaagctgca acagcagggc gcccggtgca tggattgcgg cattccgttt
      541 tgccataccg gccagctgat tagcggcatg gcctcgggct gtccggtcaa caatctgatt
      601 cccgagtgga acgatctgat ttatcgcggt ctctggcagg aggctttaca tcggctgcat
      661 aagaccaata actttcctga attcaccggc cgggtgtgcc cggcgccgtg tgaagggtct
      721 tgtgtgttgg ggctcaacga attgccggtg acgattaaga acatcgaagc ggcgattatc
      781 gacaatggat ttctccaagg ttgggtgacc gccgagccgc cggcaaagcg caccggcaaa
      841 aaagtcgcgg tgatcggttc cgggccgtcg ggcttggcct gcgcggcgca actcaaccgc
      901 gccggacatc aagtcaccgt atacgaacgg gccgatcgca tcggcggttt gctcatgtat
      961 ggcataccca acatgaagct cgacaaaaaa atcgtccagc ggcggatcga tctgatggcc
     1021 gccgagggtg tcaagttcgt tgtcaacgcc gacatcggcg cgggcatgtc tgcgcgcaag
     1081 atcaaagacg agaacgacgc tgtggtgatc tgcaccggcg cgaccaaggc tcgcgatctg
     1141 ccgatccccg gccgcgaact caagggtgtt tacatggcga tggagtttct gcgcgccaac
     1201 accaagagcc tgctcgactc ccagcataag gacggcaact atatctcggc caaagacaag
     1261 catgtcatcg tcgtcggcgg cggcgacacc ggcaccgact gcgtcggcac gtcgatgcgc
     1321 catggctgca agagtttaac ccaattggaa atcttaccgc gcccggccga cagccgacaa
     1381 gccgacaatc cctggccgga atggccgaag gtatataagc tcgactatgg ccaggaggaa
     1441 gtcgcggcgc gatttggcgc cgatccgcgt gtctatttga ccaccggcga gaaattcgcc
     1501 ggcgacgaca agggcaacgt caaagaaatg catatctacg atgtcgagtg gaccaaaaac
     1561 gagcagggcg catttattcc aaagcgagtg cccggctcag ataaagttct caaggccgac
     1621 ctggtcctgc tcgccatggg ctttctcggt ccggaagagc cggtattgac ggagctcagc
     1681 gtcgagcgcg atccccgctc caacgccaag gccgagcatg gccagtttac gaccaatgtc
     1741 gccggcgtct tcgccgccgg cgattgccgg cgcggccaaa gcctggtggt ctgggcgatc
     1801 aacgaaggcc gtggcgctgc ccgtgagtgt gatcgctttc tgatgggatt ttccaactta
     1861 ccttgattca ttcggcgggc cgcactaatg gagcggcccg ccgtgagaat tgcacgctat
     1921 tccaacatcg ttaggcgagg atttccgcgt ctggcatcac ctgattttcc ccgggttccc
     1981 cggactgccc ggtcaagctg gtttccggag agacctggca acactgacag ccgacactga
     2041 tgacttgaaa gcaatcctcg cgcattcatg gtggttacta gttattggcg tgacggcatg
     2101 gcggttgctg atttagcgta atggaagacc agggtgttac cggtctatca aaggtatctc
     2161 gcctgaaaat gccgaactca tcgtgaggtg agggcggaaa gccacggatc aggactgctt
     2221 cgtcgagcct cctgactgcc gagctgccaa aagcgaataa atagaaatca tggcagctta
     2281 gcgtcaggag tatgacgatg gaatcaccca gcgagcagaa gtttatagat cagcaaaagt
     2341 tttcattggg gtcggcggat gctagctccg gcgctgtcca atatttatcc tatgaccgga
     2401 ttctacggag tctgggatcg atgcttgagc aaaaaagtct gacggaattt gagttacagc
     2461 ttagcgatga tgtctatacc gttcgcggcc gaccgtcgcg gagactttcc cgcggttcgt
     2521 cgttgttggg aagaattttt ggctcaggtg ggactttgag aaacagccgt caaccggcca
     2581 tgattgaaca gcagtacccg gttgatgaca
//
LOCUS       DASQKS010000544         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2836136, whole genome shotgun sequence.
ACCESSION   DASQKS010000544 DASQKS010000000
VERSION     DASQKS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836136"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VE170_13900"
     CDS             <1..679
                     /locus_tag="VE170_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16584.1"
                     /translation="KRFISNAGIADFYVVFASTTPEKKAKGVSAFVIDANTSGLAVTE
                     KTQLLSPHPVGVVDFTDCGVPQTALLGAEGDGLKIALKTLDLLRCSVGAAAVGFAQRA
                     LEEAIRYSKTRRQFGRAIAEFQGIEFKLAEMATELEAARLLVYQAAWAHDCDNPDAKQ
                     KSSMAKFYATEAAQRIVDQALQIHGGNGLIAGSIMERLYRDVRALRIYEGTSEIQQLV
                     IARGLLK"
     gene            753..1742
                     /locus_tag="VE170_13905"
     CDS             753..1742
                     /locus_tag="VE170_13905"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16585.1"
                     /translation="MTKNDLVWLLAIFSVASVSIAQAQQPPKSPRTAYLTASAFSTMP
                     ARIEAFRQGLRELGYVEGKNIVIEWRSTEEKPDRLAALATELVSLKVDVIVTGGPSAT
                     RAAKGATSTIPIVMSTDPDPVANGFVASLARPGGNITGLATLAPELSGKRLELLKETV
                     PRLSRVAVLGASTTPGNAQNIKETELAARAFGLQLQYIDVQAPKDMETAFRAASKARA
                     DSIIVLSGPMVNSHRLQIVDLAVKSRLPAICPWPEYVEDGGLMSYGPSLTDLNRRAAT
                     YVDKIIKGSKPAELPVEQPIKFEFIVNLKAAKQIGLTIPLNVLVRADTVVRSS"
     gene            1790..2773
                     /locus_tag="VE170_13910"
     CDS             1790..2773
                     /locus_tag="VE170_13910"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16586.1"
                     /translation="MSKKVLSLALSAMLLALSFPVDAQQAEKIPRIGYLNATSPSTSP
                     ARVEAFRQGLRELGHVEGKNIVIEFRHAEGNLDRIPSLAAELVRLKVDVIVSGGPATT
                     RPAKEATSTIPIVMAQDNDPVATGIVASLARPGGNITGLSTLAPEISGKQLELLKEIV
                     PKLSRVAVLGTSTISSQLQTLRELDLAAGALKVKIQYLDVLDPNAIETAFQTATKGRA
                     DALLVLQSAVMNPQREKVANLAVKNRLPAIYFQNQYMEIGGLMYYGPNTPDLFRRAAT
                     YVDKILKGRKPAELPVEQPIKFEFIVNLKTAKQIGLTIPPNVLVRADRVIR"
     gene            2811..>3625
                     /locus_tag="VE170_13915"
     CDS             2811..>3625
                     /locus_tag="VE170_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ16587.1"
                     /translation="MDFDLSDELIAVRDLAREFAEKEIAPTAAKDDKEKTFRGDLVRK
                     MGELGFYGTMIPESYGGNGLGFLAMVLITEEIARVHSAMRVGINMQIGPALTLLQFGT
                     DEQKRKFIPGLMSGETIGCFAITESDAGSDVAAMRTTATKNGDRYLLNGGKLWISNGP
                     VTNGGLVYAYTDRTQKHRGMSAFFAEWNQPGLTRRTLETMGAHCSPLGELTFDNFPIP
                     VANLLGKEGDGFKICMWQLNQTRLNCAAGALGVARAAKEAALSYCNQRAQFGQ"
BASE COUNT          838 a          978 c         1050 g          759 t
ORIGIN      
        1 caagcgattt atttccaacg ccggcatcgc cgacttttat gttgtcttcg catcgactac
       61 gccggagaag aaagcaaaag gtgtgagcgc tttcgtaatc gacgcgaaca cgtcaggttt
      121 ggccgtcact gagaagactc aattgctgtc gccgcatccg gtcggcgttg tcgatttcac
      181 cgactgcggc gtgccgcaga ccgcgttact cggcgccgag ggcgacggct tgaagatcgc
      241 cttgaaaacg ctcgatcttt tacgctgcag tgttggcgcg gccgccgtcg gctttgccca
      301 acgtgctttg gaagaagcga ttcgctacag caagacgcgt cggcagtttg gccgggcgat
      361 cgctgagttt caggggattg aattcaagct cgctgagatg gcgacggaat tggaagcagc
      421 gcggctgttg gtttaccaag cagcctgggc gcatgactgc gacaatccgg atgccaaaca
      481 aaaatcctcg atggcaaagt tctacgccac cgaagcggcg cagcgcattg tcgatcaagc
      541 gctacagatt cacggcggca acggtctgat cgccggcagc atcatggagc ggctctaccg
      601 cgatgtgcgc gcgctgagga tttacgaagg cacgtcggag attcagcagt tggtgatagc
      661 gcggggatta ctgaaatagg cgcagccaaa ggctgattat ccctctgacg gagtttagtg
      721 gtcagctcag taaaaacaca ggagcgacat ccgtgacgaa aaatgatctt gtctggctac
      781 tggcaatttt ctctgttgca agtgttagca tcgctcaggc tcagcagcca cccaaaagcc
      841 cacggacggc atatctaacg gctagcgcct tttccaccat gccggcccgc atcgaagcgt
      901 tccgccaggg cttgcgcgaa cttgggtacg tggaggggaa gaacatcgtc attgagtggc
      961 gctctactga ggagaaaccc gatcgcctcg ccgcacttgc gaccgagtta gtgagtctca
     1021 aggtagatgt gatcgtcacc ggcggcccgt ccgctacccg tgccgccaag ggagcaactt
     1081 cgactattcc cattgtcatg tcgacggatc ccgatcctgt tgcaaatggt ttcgtcgcga
     1141 gcctcgcgcg acctggcgga aacatcactg gattggcaac ccttgccccg gagttaagcg
     1201 gaaagcggct ggagcttctc aaggaaactg ttcccaggct ctcccgcgtg gccgtcttgg
     1261 gggcctcgac caccccgggt aacgcgcaaa atataaaaga gacggaactt gccgcgcgag
     1321 catttggcct ccagcttcag tacatcgatg tacaagctcc caaggatatg gaaactgctt
     1381 ttcgagcagc aagcaaggcg cgtgctgact caatcatcgt gttgtctggt cctatggtga
     1441 attctcatcg actacaaatt gttgacctcg cggtaaagag tcggctcccg gcgatatgtc
     1501 cctggccaga atatgtcgag gacggtggtc ttatgagcta cggcccgagt cttaccgact
     1561 tgaaccgtcg cgctgctacg tatgtggaca agattataaa aggtagcaag cccgccgaac
     1621 ttcccgtcga gcagccgatc aagttcgagt tcatcgtcaa cctcaaagcc gcgaagcaga
     1681 tcggcctgac gattccgctg aatgtgttgg tgcgcgcgga tacggtcgtc agatcaagtt
     1741 agacagagct cagaagtcag gggtaagcaa gtgagtggtg agaaaccgta tgagcaaaaa
     1801 agttcttagt ctggctctta gcgctatgct cttggcgctt agctttccag ttgacgcgca
     1861 gcaagcggag aagattcccc ggattggata cctaaatgct acctccccct ccactagccc
     1921 cgcccgcgtt gaggcgttcc gccagggtct acgtgagctt ggacacgtgg aggggaagaa
     1981 tattgtcatc gagtttcgac atgcagaggg gaacctcgat cgtatcccgt cgctcgcggc
     2041 ggagcttgtg cgtctcaagg tcgacgtcat cgtctccggt ggcccggcaa caacgcgtcc
     2101 cgccaaggaa gcaacttcta cgattcccat cgtcatggcg caggataatg atcctgttgc
     2161 cacggggatc gtcgccagcc ttgcgcgacc tggcggtaat atcactggat tgtccaccct
     2221 tgccccggag attagcggaa aacaactgga gcttctgaaa gagatcgttc ccaaactctc
     2281 gcgtgtggcc gtgctcggga cctcaaccat ctcgagccaa ctacaaacgt tacgagagct
     2341 ggacctcgct gcaggggcgt tgaaagtgaa gattcaatac ctagacgtac tcgatcccaa
     2401 tgccattgaa accgcatttc aaaccgcaac caaaggacgt gctgatgcac tccttgtgct
     2461 gcagagcgcc gtcatgaatc cgcagcgaga aaaggttgcg aatttagcgg taaagaaccg
     2521 gctcccggca atttactttc aaaaccagta tatggaaatt ggcggcctca tgtactacgg
     2581 cccgaatact ccagacttgt tccgccgcgc ggctacgtat gtggacaaaa ttttgaaggg
     2641 caggaagccc gccgaacttc ctgtcgagca gccaatcaag ttcgagttca tcgtcaatct
     2701 gaaaacggct aagcagatcg gcctaacgat accgccaaac gtcttagtgc gagcggatag
     2761 agtgatcaga taggcagcag gcaataggca acagttatgg agaaagaaca atggattttg
     2821 atttgtcaga cgagttgatt gccgtgcgcg atttggcgcg ggagtttgcc gaaaaggaaa
     2881 ttgcaccgac cgcggcaaag gatgacaagg aaaaaacttt tcgcggcgat ctggtgcgca
     2941 agatgggcga gttgggcttt tacggcacga tgattccgga aagctatggt ggcaacggct
     3001 tgggatttct cgccatggtg ttgatcaccg aagagattgc cagggtgcac agcgccatgc
     3061 gcgtcggcat caacatgcag atcggtccgg cactgacgtt gctgcaattc ggcacagatg
     3121 agcagaaaag gaaattcatt ccgggtttga tgtcgggtga aaccatcggg tgctttgcca
     3181 tcaccgagtc cgacgccggc tcggatgtgg cggcgatgcg cacgacggca acgaaaaacg
     3241 gcgatcgcta tttgctcaac ggtggcaaac tgtggatctc caacgggccg gtgaccaacg
     3301 gcgggctggt ctatgcttac accgaccgca cgcagaaaca tcgcggcatg agcgcgttct
     3361 tcgccgagtg gaatcaacct ggcttgactc ggcgcacact ggagaccatg ggcgcgcatt
     3421 gttcgccgct gggcgaactg accttcgata attttcctat tcccgtggcc aaccttttgg
     3481 gcaaagaggg cgacggtttc aaaatctgca tgtggcagtt gaatcaaacc cggctcaact
     3541 gcgccgccgg cgcgttgggc gttgcccgag cggcgaaaga ggcggcgctg agttattgca
     3601 accagcgcgc ccagttcggc cagaa
//
LOCUS       DASQKS010000545         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2837367, whole genome shotgun sequence.
ACCESSION   DASQKS010000545 DASQKS010000000
VERSION     DASQKS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837367"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1400)
                     /locus_tag="VE170_13920"
     CDS             complement(69..1400)
                     /locus_tag="VE170_13920"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HYJ16588.1"
                     /translation="MIRIAPFRALCYSPKKIKDLSKVIAPPYDVISKEEQDKLYKKSA
                     YNFVRLDLNQKADSYDAVAQLLGEWQKQGILERDDQPAIYFSTHRFKLKGGEQKVRQG
                     FFALTELQDFATGAIKPHEKTLDAPKEDRLKLMLACHAQLSPIFVLYAQPKQTIHTIL
                     GVAVEGMTPFIETELDNGDELKLWRITDAAVIEKVQREIAEQTLLIADGHHRYEATLR
                     YRDQLRSERGPGNGNEAYNFILTYFANINDDNVVILPTHRLVRGFNHKPFLELEEALQ
                     TYFYVEQHPKTPEGKTSFLKALKNAAKKHRVIGASFKRDPRYLILRLKNKRIMQRLAK
                     DMSAPLRELDVSTLHLLVLEHILGMPPEQQVSGATIRYSQDEELVLQALENEDYQAGF
                     ILNPTKAEEIQSIVAANEKMPQKSTYFYPKLSSGLIVNKIDPQEFVVNTGL"
     gene            complement(1422..2294)
                     /locus_tag="VE170_13925"
     CDS             complement(1422..2294)
                     /locus_tag="VE170_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ16589.1"
                     /translation="MKFATIKPDGLAIVQNDTLIPITGVLAKGATMLDLIAQYDQIKG
                     ALADLAAKGGGVKLDAKLLKPPVERPSKIYAAAGNYKRGTGGLDAGRGRGAASTISPE
                     ELLENIFLKPPSAIAGPEDNIVIPKDAESIFPELELCVVIGKKVRHLSKSQAMDAVFG
                     YTIMQDVTARGYGKSKNMGGTRSVRKGFETFAPIGPWITTKDEIADPHNLWMKLWING
                     ELKQSAKTDAMINDIVTQISFLSQVTTLYPGDLLTTGNPDSPEFQEKLKPGDVVRSEI
                     EGIGAMNQGVAKES"
     gene            2506..>3007
                     /locus_tag="VE170_13930"
     CDS             2506..>3007
                     /locus_tag="VE170_13930"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HYJ16590.1"
                     /translation="MTHSWLHLTVHTSAACLDAVANFLTESGSSGVVLKPRAVEAYFA
                     NNRSTSALKRAVQKFVMDAVKLQERSSKPRLLWQIARPEDWENSWKRFIRPSRVGKQF
                     WVTPPWLTPPRFRSRQVITIEPGMAFGTGTHATTRGCLEFIEEVAALCASVKLTALDV
                     GTGSGIL"
BASE COUNT          565 a          784 c          888 g          770 t
ORIGIN      
        1 tgcggaagtc cccgtggact atccgcattt tttttggcgc ggtgcaaatc ccgaagagtg
       61 cacgtggttt ataaaccggt atttacgacg aactcctgcg gatcgatctt attgacgatc
      121 aggcccgagc ttagcttggg gtaaaagtaa gtcgacttct gcggcatctt ttcattggcg
      181 gcgacgatgg attggatctc ttctgccttg gttgggttca agataaaccc ggcttggtag
      241 tcttcgtttt ccaatgcctg caatactaat tcttcgtcct gcgagtagcg gatggtggcg
      301 ccgctgactt gctgctcggg gggcatgccg aggatatgct ccaagaccaa tagatgcagc
      361 gtgctgacat cgagctcgcg gagcggcgcg ctcatgtctt tggccaagcg ctgcatgatg
      421 cgtttgtttt tcagccgcag gatcaaataa cgcggatcgc gcttgaagct ggcgccgatg
      481 acgcggtgct tcttcgccgc gttcttgagc gctttgagaa acgaagtctt gccttcaggc
      541 gtcttcgggt gctgctcgac gtaaaaatag gtttgcagcg cttcttccaa ttccaaaaat
      601 ggcttgtggt tgaagccgcg cacgagccga tgggtcggca atatgacgac gttgtcgtca
      661 ttgatattgg cgaaataggt caagataaag ttgtacgctt cattgccgtt gcctgggccg
      721 cgctcgctgc ggagctgatc gcggtagcgc agcgtggctt cgtagcgatg gtggccgtcg
      781 gcgatcagca gggtctgctc ggcgatttcc cgctggactt tttcgatgac cgccgcatcg
      841 gtgatccgcc agagcttcaa ctcgtcgccg ttgtccagct cggtttcgat gaacggcgtc
      901 atgccctcga cggcaacgcc gagaatggta tggatcgtct gtttcggttg ggcgtagagg
      961 acgaaaatcg ggctcaactg ggcgtgacag gcgagcatga gcttcagccg atcttctttg
     1021 ggcgcgtcca gggttttttc gtgaggtttg atcgcgccgg tggcaaaatc ttgcagttcg
     1081 gtcaaagcga agaagccctg ccggactttc tgctcgccgc cttttaactt gaaacgatgc
     1141 gtggagaagt aaatcgccgg ctgatcatct cgttcgagaa tgccttgttt ctgccattcg
     1201 ccgagcaact gggcgacggc gtcataggag tccgcctttt gatttaaatc gagccgaaca
     1261 aaattatagg ccgacttttt gtagagcttg tcttgttctt ccttggagat cacgtcgtac
     1321 ggcggcgcga tcactttgct caagtctttg attttttttg ggctgtaaca gagtgctcga
     1381 aaaggcgcta tacgaatcat tatgtttaat ccgctatgtg attagctttc cttggccacg
     1441 ccttggttca tggcaccgat gccttcgatc tccgacctca cgacatcgcc tggtttcagt
     1501 ttctcttgaa actccggtga gtccggattg ccggttgtca gcagatcgcc ggggtagaga
     1561 gtcgtcacct gggaaagaaa gctgatttgc gtgacgatgt cgttgatcat ggcgtcggtc
     1621 ttggccgact gcttgagttc gccgttgatc cacagcttca tccagagatt atgcggatcg
     1681 gcgatttcat ctttggtcgt gatccatggg ccgatggggg cgaaggtctc gaatcctttg
     1741 cgcacgctgc gcgtaccgcc catatttttg ctcttgccgt agccgcgcgc ggtgacatct
     1801 tgcatgatgg tgtagccgaa cacggcatcc atcgcttgtg atttgctcag atggcggact
     1861 tttttgccaa taaccacgca aagctcgagc tctggaaaga tcgactcggc atccttcggg
     1921 atgacgatat tgtcttccgg cccggcgatg gcggacggcg gctttaagaa aatattctcc
     1981 aaaagctctt ccggtgaaat cgtcgatgcg gcgccacggc cgcggccggc gtcgagtccg
     2041 ccggtgccgc gcttgtagtt gccggcggca gcatagattt tcgacggccg ctccaccggt
     2101 ggcttgagca atttggcgtc gagcttgaca ccgccgccct tggcggcgag gtcggctaag
     2161 gcgcctttga tttgatcgta ttgcgcgatc aaatcgagca tggtcgcccc cttggctaag
     2221 acgccggtga tgggaatcag cgtgtcgttt tggactatgg cgaggccatc aggtttgatc
     2281 gtggcaaatt tcatcggcag ttcctttcag tgtaactatt ttcaatatct ggaagtagcg
     2341 attttgacaa gctggctagg gccaatgcgc gcgaattgtt aactcgtttt tcaagttcgg
     2401 cggtttgcgc cggtgcggac gagattcccg gcggcgctcg acttgaccgc gagcaagtct
     2461 cagtcgatca attgatccac tgacttcgct gtggcatata aaatcgtgac acattcttgg
     2521 ttgcacctca cggttcatac cagcgccgcc tgtctggacg ccgtggcgaa cttcttgacc
     2581 gagagcggct cgtctggcgt cgtcttgaaa ccccgcgcgg tggaagctta tttcgctaat
     2641 aatcgtagca ccagtgcgct caagcgggcg gttcaaaaat ttgtcatgga tgctgtcaaa
     2701 ctccaggaac gcagcagcaa gccgcgcctg ttgtggcaga tcgcccggcc cgaagattgg
     2761 gagaactcct ggaagcgatt tatccggccg agccgggtcg gcaaacagtt ttgggtgaca
     2821 ccgccctggc taacgccgcc gcggtttcgc tcgcgccagg tgattaccat cgagcccggc
     2881 atggcttttg gcaccggcac ccatgcgact acgcgcggct gtttggaatt tatcgaggag
     2941 gtggcggcgt tgtgtgcatc agtgaaactc accgccctcg acgtcggcac cgggtcggga
     3001 atcttgg
//
LOCUS       DASQKS010000546         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2837708, whole genome shotgun sequence.
ACCESSION   DASQKS010000546 DASQKS010000000
VERSION     DASQKS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837708"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1242
                     /locus_tag="VE170_13935"
     CDS             268..1242
                     /locus_tag="VE170_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HYJ16591.1"
                     /translation="MRTNLFSIVAGLALLGTAPLDAQEFPNKVISMVVPFAAGGPTDT
                     VARLIGVPMSKTLKQQVIVENVGGAGGTIAANRVAKASPDGYTILIHHIGMSTAPALY
                     RKLPYNPTTDFEPIGMINEVPMTLVAKNDFPAKDLKELIAYVKANKEKINYANAGIGA
                     ASHLCGMLFMSAIQTDVTTVPYSGTAPAMNDLLGGQVDFMCDQTTNTTSQIKAGKIKV
                     YGVTTKKRVASLPNVPTLDEAGLKGFEVSIWHALYAPKSTPKPVVDKLTNALQDAMKD
                     STVKQRFADLGTEPVTLNRATPAALSAHLKAEIDRWAPIIKKAGVYAD"
     gene            1363..1815
                     /locus_tag="VE170_13940"
     CDS             1363..1815
                     /locus_tag="VE170_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ16592.1"
                     /translation="MTRNPKDFWTGLLYIFFGASAIIIARDYNMGTALRMGPAFFPTI
                     LGAVLAVIGAIAVIRSFIAAGAPIGGFSFKGLVLVTLSVVIFGVLVRGAGLVVALPLL
                     VIISAYASTRFRWRTTLIMAAGLMTFCALVFVKGLGIPLPIIGAWLGG"
     gene            1846..3345
                     /locus_tag="VE170_13945"
     CDS             1846..3345
                     /locus_tag="VE170_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ16593.1"
                     /translation="MELLDNLLLGFQTALTLSNLFYCFLGVFLGTAIGVLPGLGPTAT
                     IAMLLPITFVLPPVTALIMLAGIYYGSQYGGSTTSILVNLPGEAASVVTTLDGYQMAK
                     QGRAGAALAASAIGSFFAGTVATLLIAAFGPPLAAVALQFGPAEFFSLMVLGLVASVV
                     LAQGSLLHAIGIVVLGLLLGLIGTDVNSGTQRFTFGFFELADGIGFVTVAMGMFGLGE
                     IIRNLENEADRSVMLTKITGLWPTRDDFKRMVAPILRGTVIGSALGILPGSGSILGSF
                     AAYSIEKKISKHRAEFGKGAIEGVAAPEAANNAGAQTSFIPLLTLGIPSNPVSALMVG
                     AMIVHGIQPGPSVINEQPALFWGMIVSMWIGNLFLVVLNYPLIGMWVRMIMIPYHLLY
                     PGILVFCAIGVFSLKNATFDIYLMAVFGIVGYVFAKLGAEPAPMLLAFILGPLMEEYL
                     RRAMLLSRGDPMVFWHRPISATLLLLAALAMLAVLLPSFSKTREEAFKE"
     gene            3458..4336
                     /locus_tag="VE170_13950"
     CDS             3458..4336
                     /locus_tag="VE170_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16594.1"
                     /translation="MGTIFKEVNTLSFIGNVGPKTERVWKEVDEENDIVGCQGKHERA
                     CQLLTPLKLLQADLPGDGETKQLPIFVNDDVRIDLQYCPGAKGTNGHRPTGFYETQIQ
                     VQNSRVTNSSEGDFVLDEGDVLVLPPAVSHTNSGSGPTTRLIVYTRSPMQIGRSYPVK
                     ESVVANKQSILLKPKTVLDQIGEGAASGGKHFELVENADIMIETTHRSDAQKIYHRGF
                     GQDEIAFQLTGRRATRTSQGDYMLDTADFLLIPPGTSHRNIGDMATIRVILYTRKTVR
                     LADEYVERARMAGQQR"
     gene            4344..5372
                     /locus_tag="VE170_13955"
     CDS             4344..5372
                     /locus_tag="VE170_13955"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16595.1"
                     /translation="MNIKNFWILPIPYRLASKSMKAIGLTALATVASILALTKVPAAA
                     TLESIKVSYAVLSAAYMDHVTAMEKGYFRDEGLNVEVLRMGGGVATPALLSGQLHFSS
                     SAGSSLSAAVRGGPVKIVYTNLSRPSYYLVATNPNIVTAKDLVGKKVVINSFGDTGHL
                     ATLMYLRKQGINPASVLFITVGRNEVRLPALMSGMVDATPLAPRELVKLGLQKQKVLA
                     DLGKEIQLVWNGVAVSSKLLADNPALVERFLRGIVRGREYARRNRDETIAIIGKHDPS
                     PIEGLRADYEVTKSSMTDEGWLPDDILREEVAIRAELTKVALPSNPLGMYDYSIVKKI
                     YAQLKSSR"
     gene            5514..5654
                     /locus_tag="VE170_13960"
     CDS             5514..5654
                     /locus_tag="VE170_13960"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HYJ16596.1"
                     /translation="MKGAKPAELPVEQPMKLEFIINLKAAKQIGLTIPPNVLARADKV
                     IR"
     gene            complement(5676..>6326)
                     /locus_tag="VE170_13965"
     CDS             complement(5676..>6326)
                     /locus_tag="VE170_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16597.1"
                     /translation="GRIILGVSVGPVRDYIQRQFTACGVPPQEKAGRLSETIEIMRRL
                     WSETKIDYEGRYFKLHDVGILPHPAQRPGIPIWIVADRNDTGFKRVARLGDGWVTLAP
                     TLERFTGARAKIAAHAESYGRVDKLRESALYVTFNISQDAEKAKEQGWQWMENFFATP
                     RAKLGHFFTIFATPEECVGLLKGYIDAGLTTIIARIASDDVPGQARMLLNEIRPAL"
BASE COUNT         1322 a         1774 c         1818 g         1412 t
ORIGIN      
        1 gcggttggct ttgtctaata aactttctag gcttgctggg ttatttcgcc acggaaggat
       61 ggctcgattt attagtaatt acgcgaataa atggtccgct tttcccagtc gtgcgacaat
      121 tctgccagat atgcctatgg cgcgtggcat tacgctggca tggcacttgc gccaagtgtc
      181 gttggtttga cgattctgag cgtctaaggg ttacgctgtg aaatgaatag ccggggcatt
      241 attatattat tataaggagg agacattatg agaacgaatc tgtttagcat cgtagcaggg
      301 cttgccttac taggtaccgc tccgttggac gcccaggaat tcccgaacaa ggtcatcagt
      361 atggtggtac cgttcgccgc gggcggcccg acggacaccg tggcgcgcct gatcggtgtg
      421 ccgatgagta agactcttaa gcaacaggtt attgtagaga atgtcggcgg cgccggcgga
      481 acaatcgccg caaaccgagt cgcgaaagca tcaccggacg gctacacgat actcatacac
      541 catatcggca tgtccaccgc gcccgccctg tatcgcaagc tgccttataa cccaacgacc
      601 gacttcgagc ccattggtat gatcaacgaa gtgccgatga cgctggtggc aaaaaacgac
      661 tttccggcca aggatctgaa ggagctgatc gcttatgtta aagccaacaa ggagaagatc
      721 aattacgcca acgccggtat cggcgccgcc tcgcatctgt gcgggatgct gttcatgagc
      781 gcgatccaga cagacgtcac caccgttcct tatagcggaa cggccccagc catgaatgat
      841 cttctcggcg gccaggtgga cttcatgtgc gatcaaacta caaacacgac cagtcaaatc
      901 aaagcgggaa agatcaaagt ctatggcgtg acgactaaga aacgtgtcgc ttcgttgccc
      961 aatgtcccga ccctggacga agctgggttg aaaggcttcg aggtttcgat ctggcatgct
     1021 ctctacgcgc caaagagtac gccgaagccg gttgtcgata agcttaccaa tgcgctgcag
     1081 gatgccatga aagattcgac ggtgaaacag cgtttcgccg acctgggtac cgaacctgtg
     1141 acgctaaacc gagcgacccc cgcggcgttg agcgcgcacc tcaaagcaga gatcgacaga
     1201 tgggcgccaa tcatcaagaa ggcaggagtt tatgccgact aattggcatt catcataggg
     1261 caatttaaag gccgggtttc gtcccggcct ttttttgctt cttttcatgg ttggtctgat
     1321 gtacaaggcg cattagcgac ggtagtagtt taaacactct atatgacgcg caatcccaaa
     1381 gatttctgga ccggcttgct gtatatcttc ttcggcgcga gcgcgattat tatagcccgt
     1441 gactacaaca tgggaacggc cctcaggatg ggcccagctt tttttcccac tattctcggc
     1501 gcggtgctcg ccgtcatcgg cgcgattgca gtaattcgat cctttatcgc ggccggcgcg
     1561 ccaatcggtg ggttttcgtt caaggggttg gtgctggtca cactatcggt cgtcatcttt
     1621 ggcgtcctag tgcgcggcgc gggattggtc gtcgccttgc cgctgctcgt aatcatcagc
     1681 gcgtacgcga gcacgcgctt ccgatggcgc acgacgctca tcatggcggc gggactgatg
     1741 accttttgcg cgctcgtctt tgtcaaaggc ttaggcatcc cgctgccaat catcggtgct
     1801 tggttgggcg gttaacggcc atgattatta gcagggcggg caacgatgga actactcgat
     1861 aatctgctcc tgggttttca gacagccctg actctcagta atttgtttta ctgtttcctc
     1921 ggcgtttttc tcggcacggc gatcggcgta ctgcccggtc ttggtcccac ggccactatc
     1981 gccatgctgc tgccgattac gttcgttttg ccaccagtga ccgcgctgat catgctcgcg
     2041 ggtatttatt acggatcgca atatggcggc tcgacgacat cgattctggt taatcttccc
     2101 ggcgaagccg cttcggtagt tacgactctc gacggctacc agatggcgaa gcaaggcagg
     2161 gcgggagcgg cgcttgcggc ttcggcgatc ggttcgttct tcgccggcac cgtcgccacg
     2221 ctcctcatcg ctgccttcgg cccgccgcta gccgccgtag cgttgcaatt tggtccggcg
     2281 gaatttttct cgctgatggt tctcggcctg gtggcttccg tggtgctagc gcaaggctcg
     2341 ttgctccacg ccatcggcat cgttgtgctc ggcctgctct tgggtcttat cggcaccgat
     2401 gtcaattccg gcacccagcg ctttaccttc ggtttcttcg aactcgccga cggtatcggt
     2461 ttcgtcaccg tggccatggg catgtttggc ctgggcgaaa tcattcgcaa tctggaaaac
     2521 gaagcagacc gatcggtaat gctcactaaa attaccggac tttggcccac ccgtgacgac
     2581 ttcaagcgca tggttgcgcc aatactccgc ggcacggtga tcggctcggc attgggcatt
     2641 ctccctggca gcggctcgat cttgggctcc ttcgccgctt attccatcga aaagaagatc
     2701 tccaagcatc gcgccgaatt cggcaagggc gcgatcgaag gggttgccgc gccggaggcg
     2761 gctaataatg ccggcgcgca aacctccttt attccactgc tcactttagg gattccgtcc
     2821 aatccggtca gcgccctgat ggtcggagcc atgattgtcc acggcatcca gcccggaccg
     2881 tcggtgatca acgaacagcc ggcgcttttt tggggcatga tagtgtcgat gtggatcggt
     2941 aatctatttc tagttgttct taactacccg ctgatcggca tgtgggtgcg catgattatg
     3001 attccttacc acctgctata tccgggcatc ctggtcttct gcgccatcgg tgtattctca
     3061 ctaaaaaatg ccacctttga catatacttg atggcggtgt tcggtatcgt cggttatgtt
     3121 tttgccaaac tcggcgccga accggcgccg atgctgctgg cctttatcct tgggccattg
     3181 atggaagagt atctgcgccg tgccatgctg ttgtcccgcg gcgacccgat ggttttttgg
     3241 caccggccaa tcagcgcgac cttgctcctc cttgccgcgc tggcgatgct cgcagtcctg
     3301 ctgccgtcgt tcagtaagac cagggaagaa gctttcaaag aatgatgccg cgcagcgcta
     3361 tcggccgcga tcttctgcgt aatcgatcac accgagtttg acagccaatt tggctgttgt
     3421 tacagaccgc gatgaccgcg gccgctagga ggagaccatg ggaacaattt tcaaggaagt
     3481 gaatacgctt tcgtttatcg gcaacgtcgg acctaagacc gagcgggtat ggaaggaagt
     3541 cgacgaagag aacgacatcg tcggctgcca gggaaagcat gagcgggctt gccagctgct
     3601 gacgccgcta aaattgctgc aagccgatct gcccggtgat ggcgaaacca aacagctgcc
     3661 gatattcgtc aacgatgacg tgcgcatcga cctgcaatat tgccccggcg ccaagggcac
     3721 caatggccat cggccgacgg gattttatga aactcagatc caggtgcaga actcccgggt
     3781 gaccaacagt tccgagggcg acttcgtcct cgacgaaggc gacgtgctgg tattgccgcc
     3841 ggcggtgagc catacaaaca gcggtagcgg cccgaccacg cggctgatcg tctacacgcg
     3901 ctcgccgatg cagatcgggc gcagctatcc ggtcaaggaa agcgtcgtag cgaacaagca
     3961 gagcatccta ctcaaaccga aaaccgtcct cgaccagatc ggcgaaggcg cggcctcggg
     4021 cggcaaacat ttcgagctgg tggaaaacgc cgacatcatg atcgaaacca cgcatcgttc
     4081 cgacgcgcag aagatttacc accgcggctt cggccaggac gaaatcgcgt tccagttgac
     4141 cggccggcgc gccacgcgca ccagccaggg cgactatatg ctcgacacgg cggattttct
     4201 cctgatcccg ccgggcacgt cgcaccgcaa tatcggcgat atggcgacca tccgggtgat
     4261 tctctacacg agaaaaaccg tgcggcttgc cgatgagtat gtcgagcgag caagaatggc
     4321 aggacaacag aggtagctga gcagtgaata ttaagaattt ttggatcttg ccaattcctt
     4381 atcgtctagc cagtaaaagc atgaaggcga taggtctgac ggcgcttgca acagttgcgt
     4441 cgatccttgc tctcacgaag gtgcccgctg ccgcgacatt ggagagcatc aaagtctcct
     4501 acgccgttct gagcgccgcc tatatggacc atgtgacggc gatggagaaa ggctacttcc
     4561 gtgacgaagg attgaacgtc gaagtgctgc gcatgggcgg cggggtggcg acgccggcgc
     4621 tgctctcggg gcagttgcat ttcagcagct cggcgggctc gtcgttgagc gcagcggtgc
     4681 gcggcgggcc ggtgaagatc gtttacacca atttatcgcg ccccagttat tatctggtcg
     4741 cgaccaatcc caatattgtc acggcgaagg atctcgtcgg caaaaaggtg gttatcaaca
     4801 gtttcggcga caccggccat ttggcgacgc tcatgtatct caggaagcaa ggcatcaatc
     4861 ccgcctccgt tctgttcatc accgtcggcc ggaacgaggt tcggctcccg gcgttgatgt
     4921 ccggcatggt cgacgccaca cccctggcgc cgagagaatt ggttaagctt ggtttgcaaa
     4981 agcaaaaggt cctggccgat ctcggcaaag aaattcagct ggtctggaac ggcgtcgcgg
     5041 tatcgagcaa gctcttggcg gacaatcccg cgttagtgga gcgcttcttg agagggatcg
     5101 tccgaggccg cgaatacgcg cgccgcaacc gcgacgaaac catcgcgatt atcggcaaac
     5161 acgatccctc gcccatcgaa ggtctgcgtg ccgactatga ggttaccaag agcagcatga
     5221 ccgacgaggg ctggctacca gatgacatcc tgcgcgaaga agtcgcgatc cgcgccgaac
     5281 tcaccaaagt cgctctcccg tccaatccgc tcggcatgta tgattacagc atcgtcaaga
     5341 agatctatgc tcagctaaaa tcaagccggt aaaatacccc gagtcgatgc ggtaaagaac
     5401 cggctgccgg cgatctattt tcggcgggaa attgttgacg ccggtgggct catgacctac
     5461 agcccgagcc aagctgactt ggcccgccgc gcccgacgta tgtcgacaag attttgaaag
     5521 gcgccaagcc cgccgagctt ccggtggagc agccgatgaa gttagagttc atcattaatc
     5581 tgaaagcagc gaagcagatc ggtctgacga ttccgccgaa tgtgctggcg cgggcggaca
     5641 aggttattag gtagtttttg gatttggagc tacgctcaca gcgccgggcg gatttcattt
     5701 agcagcatgc gtgcttggcc gggtacgtcg tcggaggcga tgcgcgcgat gatcgtcgtc
     5761 agcccggcgt cgatgtagcc tttcagcaaa cctacgcatt cttcgggtgt ggcgaaaatc
     5821 gtgaagaagt ggccaagttt agcgcgcggc gtggcgaaga aattctccat ccattgccag
     5881 ccttgctctt tcgccttctc cgcgtcttgg gaaatattga aagtcacgta gagcgcagat
     5941 tcacgtagtt tatccactcg cccatagctt tcggcgtgcg ccgcgatctt cgctcgggcg
     6001 ccggtgaatc gttcgagcgt cggcgccagc gtgacccaac cgtcgcccaa gcgggcgaca
     6061 cgtttgaaac cggtgtcgtt gcggtcggcg acgatccaaa tgggaatgcc gggccgttgc
     6121 gccggatggg gcaggatgcc gacatcgtgc agtttgaagt agcgaccctc gtaatcaatc
     6181 ttggtctcgc tccacagacg gcgcataatc tcgatggttt cgctgagtct tccggccttt
     6241 tcctggggcg gcacgccgca agcggtaaat tggcgctgta tatagtcgcg caccggcccg
     6301 acgctgacgc ctaaaataat cctgcc
//
LOCUS       DASQKS010000547         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1156652, whole genome shotgun sequence.
ACCESSION   DASQKS010000547 DASQKS010000000
VERSION     DASQKS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156652"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VE170_13970"
     CDS             <1..208
                     /locus_tag="VE170_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16598.1"
                     /translation="LDIVKDADYLIDLGPEGGAQGGQLVACGAPLDMIKDGKKSYTAR
                     FLRDYLNGAATPMAKKPARARASV"
     gene            210..599
                     /locus_tag="VE170_13975"
     CDS             210..599
                     /locus_tag="VE170_13975"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HYJ16599.1"
                     /translation="MASFFRMLGHGLRLRCPRCGIGRLYGKPFRMNEHCAHCGLKFER
                     EQGYFVGAIYINYTATIAVAVPGFFLLDAFAGIDINQQLAFWIPFALIFPLLFFHHSR
                     SLWLVLDHLVNPDKQLFSVPPKKTPDP"
     gene            complement(603..932)
                     /locus_tag="VE170_13980"
     CDS             complement(603..932)
                     /locus_tag="VE170_13980"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HYJ16600.1"
                     /translation="MNRKDESFNDFILDQLSEMDSVEARRMFGGHGLYQDETFFGIVH
                     KGKLFFKIDDNTVGEYRKRKMKPFRPNGKQTLKSYYQVPVEIIEDADALRQWAVKAVQ
                     CQEKGSR"
     gene            complement(993..1853)
                     /locus_tag="VE170_13985"
     CDS             complement(993..1853)
                     /locus_tag="VE170_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYJ16601.1"
                     /translation="MTGFALTLILGAAVIHATWNLINKQASGHATFTWIVAVLSALFY
                     APATIAIIEIWQLKFSFVEVGMIAGSAALHTGYFLLLNQAYRIGDLSLVYPLARGTGP
                     LLSTLAAILFLGERPSAVALLGALLIIGGVMVLAGNFTRLRQGGTRTAVGYALITGLF
                     IAGYTLWDKQAVSHFAIMPMVLDWGANVGRAILLTPFALHYSEAAVIEWREHKWEAVA
                     VAILIPLSYILVLTAMSFTPVSYVAPAREISILIGTALGARLLAEGDARRRLAAASAM
                     VLGIVGLALG"
     gene            complement(1875..2297)
                     /locus_tag="VE170_13990"
     CDS             complement(1875..2297)
                     /locus_tag="VE170_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HYJ16602.1"
                     /translation="MQSIAIGTAVAGRGLEGDRYFKQTGTYSNLPGTGREVTLIESEA
                     VVALAREYDIQLPPGLARRNIVTRGIALNHLVGKTFSVGAAVLRGMRLCQPCLHMERL
                     AVKGAAHGLIHRGGLRAAIVSGGEILVGARIFPAEETG"
     gene            complement(2366..4006)
                     /locus_tag="VE170_13995"
     CDS             complement(2366..4006)
                     /locus_tag="VE170_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HYJ16603.1"
                     /translation="MATPYREKLDKIRMAFPQPVSDRVHDSYFVHSIMRALDMVDGLK
                     SEVPVLGPREPLDYGAAQMARLPEGSSTVEDVTVNLVDYFKGLTIWGHPRTQINVVPP
                     PSISSLIATLLTGLYNPNLAWDEYSHKIALAEVEAVAMSAVLMGYDPARASGVFTFGG
                     TGTTLYGVKVGLEKAIPGAMQNGVGAGAMLFASDTSHYCRYNIAGWLGLGAKNLITIP
                     SDSRNAIKIDVLRDHARAALESGWPIAAFIATMGTTDAFGIDDLEAMVHMRDELVSEY
                     KLSYRPHIHADAVIGWAWQAFADYDFENNPLEFRPRTVRALAGACQRIRHLHLADSIG
                     IDFHKTGFTPYISSLVLFKERDDLRLMMRSQEQMPYLYQFGEHRPGMFTLETSRSGMG
                     ALAALANLKLFGKEGLRVVIGHIVEMTQLLREHLEGHAATAVLNRDNFGTVTLFRAYP
                     DGVDTFAIKDQEMDDENFRPTLLRHNDYNRKIFDYVHGEAMAGRGVVISLTDSYRETG
                     YGEPVTALKSFILSPFTDEENVESVVDKVLEARGKVKV"
     gene            complement(4015..>4794)
                     /locus_tag="VE170_14000"
     CDS             complement(4015..>4794)
                     /locus_tag="VE170_14000"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HYJ16604.1"
                     /translation="ILVMPGAYDVLSALLFQQLGFQAIQGTSGGIAAALGYPDGEAMS
                     RELFVQLTASFAAAVSLPVNADGEKGYGDASGIKDTVRALVAAGVAGMNLEDSIAKGG
                     SGLVELGQQLEKIKAVMDAKRELGSEFFLNARVDSFHVLTNDPKKALDEAIRRGNAYA
                     EAGGDCIFYLNLHAAETIGTIAKEVKAPVSILAGPQSPSVKELQDLGVARVSYGSGFM
                     KAAITGSKKLAQEILEKGTCSLLKDGVQTPEINALVSKKRG"
BASE COUNT         1010 a         1508 c         1311 g          965 t
ORIGIN      
        1 cctcgacatc gtcaaagacg ccgactacct gatcgatctg ggccccgaag gcggcgctca
       61 aggcggtcaa ctagtcgcct gcggcgcgcc attggatatg atcaaagacg gcaaaaaatc
      121 ctatacggcg cgcttcctgc gtgactatct caacggcgct gccaccccga tggcaaagaa
      181 acccgcccga gcgcgcgcga gcgtttaaag tggcatcatt cttcaggatg ctcggccatg
      241 gcctgcgcct gcgctgcccg cgctgcggca tcggccgctt gtatggcaag ccatttagga
      301 tgaatgagca ctgcgcccat tgcggactaa aattcgagcg cgaacagggc tacttcgtcg
      361 gcgccattta catcaactac accgctacca tcgccgtcgc cgtgcccggc ttctttctcc
      421 tcgacgcctt cgcgggtatc gatatcaacc aacagctcgc cttctggatc ccgttcgcgt
      481 tgatcttccc gctgctgttt tttcatcact caagaagttt gtggctggtg ctggatcatc
      541 tcgtcaaccc ggacaagcaa ctgttcagcg ttccgccgaa aaaaacgccg gacccctaac
      601 cttcatctgc tacccttctc ctgacactga accgctttga ccgcccactg ccgcaacgcg
      661 tccgcatctt cgataatctc cactggcacc tggtagtaac tcttgagcgt ctgcttgcca
      721 ttggggcgaa acggcttcat cttgcgcttg cggtattcgc cgacagtatt gtcatcgatc
      781 ttgaagaaca gtttgccctt gtgaacgatg ccgaagaaag tctcgtcctg atacaagcca
      841 tgcccgccaa acatccgccg cgcctcgacg ctgtccattt cgctcaactg gtcgagaata
      901 aaatcattga acgattcatc cttcctattc atgagacgtc tgagctccga accccttgaa
      961 cctttgaacc cttgaacttt tgaacggatg ttttaaccga gggccaaccc gacgatcccc
     1021 aacaccatag cgctggccgc cgcaagccgg cgacgcgcat ccccttcggc gagcaagcgc
     1081 gcgcccagcg ccgtgccgat aaggatgctg atttcgcgcg cgggcgcgac ataactcacc
     1141 ggcgtaaagc tcatggcggt cagcacgagg atatacgaga gcgggatcag gatcgcgacg
     1201 gcgaccgcct cccatttatg ctcgcgccac tcgatgacgg ctgcctcgga atagtgcagg
     1261 gcgaagggcg tcagtaaaat cgctctacca acgttggcgc cccagtccaa aaccatcggc
     1321 atgatggcaa aatggctcac cgcctgcttg tcccatagag tatagccggc gatgaataaa
     1381 ccggtgatca gcgcatagcc gaccgcggta cgcgtgccgc cctggcgcag gcgtgtaaag
     1441 ttgccggcca acaccatcac accgccgatg atcaagagcg cgcctaggag ggcaacggca
     1501 gacggccgct cgccgagaaa tagtatcgcc gccaaggttg atagcagcgg cccagtgccg
     1561 cgcgccaacg gataaaccag cgacaagtcg ccgatccggt atgcttgatt gagaagcaag
     1621 aaatagccgg tgtgcagcgc cgcgctgccg gcgatcatgc cgacctcgac gaagctgaac
     1681 ttcagctgcc agatttcaat gatcgcaatc gtcgccggcg cgtagaataa cgccgacaac
     1741 accgcgacga tccaagtgaa tgtcgcatgg ccactcgcct gcttattgat cagattccag
     1801 gtcgcatgga tgaccgcggc gccaagaatt agcgttaggg caaaacctgt catggcatca
     1861 tcccgcggcc ccgatcaacc cgtttcctct gctggaaaaa tccgcgcgcc gaccaggatc
     1921 tcgccgccgc tgacgatggc cgcgcgcagc ccgccgcgat gaatcaaccc atgcgcggcg
     1981 cccttgacgg cgagtctttc catatgcaag caaggctggc acaggcgcat gccgcgcaga
     2041 accgcggcgc cgacagaaaa cgttttcccc accagatgat tcagcgcaat accgcgggtg
     2101 acgatattgc gtctcgccaa tcctggcggc aactggatat cgtattcgcg cgccagcgcg
     2161 acaacagctt cactctcgat caatgtcact tcacgtcccg tgcccggaag gttcgagtat
     2221 gtgcccgtct gtttgaagta gcgatcacct tcaaggcctc gtccggcgac agcagtgccg
     2281 atagcaatgc tttgcattgg cgccgcccca tgtggggcga tgtgtaggga gacaactaaa
     2341 ccgtgcgtcg gcatcgtttg cgctgtcaga ccttgacctt gccgcgcgct tccaaaactt
     2401 tgtcgaccac cgattcgacg ttctcctcgt cggtgaaagg tgagagaata aacgatttca
     2461 acgcggtcac cggctcgccg taaccggttt cccggtaaga gtcggtgagc gagatcacca
     2521 caccccggcc ggccatcgct tcgccgtgga cgtagtcgaa aatcttgcgg ttataatcgt
     2581 tgtggcgcag caaggtaggg cgaaaatttt cgtcgtccat ctcctggtct ttaattgcaa
     2641 acgtgtcgac accgtcgggg taggcacgga acagggtcac cgtgccgaag ttgtcgcggt
     2701 tgagcaccgc ggtggcggca tggccttcaa gatgttcgcg cagcagctgg gtcatctcga
     2761 cgatatggcc gatcacgacc cgcagacctt ccttgccgaa cagttttaga ttggccaacg
     2821 ccgccagcgc gcccatgccg ctgcgcgagg tctcaagagt gaacatgccg ggacgatgct
     2881 cgccgaactg atagagatac ggcatctgct cttgactgcg catcatcagg cgcagatcgt
     2941 cgcgctcttt gaacaacacc aggctcgaga tatatggcgt aaaaccggtc ttgtgaaagt
     3001 cgatgccgat ggagtcggcc agatgcagat ggcgaatgcg ttggcaggcg cccgccagag
     3061 cgcgcaccgt gcgcggacga aactccaacg ggttgttctc gaaatcgtaa tcggcgaagg
     3121 cttgccaggc ccagccgatg accgcatcgg catggatatg cggccgatag gacagcttgt
     3181 attcgctgac tagctcgtca cgcatatgga ccatggcctc gagatcgtcg ataccgaacg
     3241 catccgtggt tcccatggtg gcgatgaagg cggcgatggg ccagccgctt tccaaagccg
     3301 cccgcgcgtg atcgcgcagc acgtcgatct tgatcgcatt gcgcgagtcg ctgggaatcg
     3361 tgatgagatt tttcgcgccg aggccgagcc atccggcgat gttgtaacgg cagtagtggc
     3421 tggtatcgct ggcgaacagc atcgcgccag cgccgacgcc attttgcatg gcgccgggta
     3481 tcgccttctc cagaccgact ttaaccccat agagcgttgt cccggtgcca ccgaaggtaa
     3541 agacgccgga agcacgcgcc ggatcgtagc ccatcagcac cgcggacatt gccactgctt
     3601 cgacttcggc gagagcgatt ttgtggctat actcgtccca cgccagattg ggattataga
     3661 gtccagtcaa gagcgtcgcg atcaatgacg aaatcgacgg cggcggcacc acattgatct
     3721 gggtgcgcgg gtggccccag atcgtcagac ctttgaaata atcgactagg tttaccgtca
     3781 catcctccac cgtcgacgaa ccttccggca accgcgccat ttgcgccgcg ccataatcca
     3841 gcggctcccg cggccccagc accggcactt cgctcttgag gccatcgacc atgtccaacg
     3901 cgcgcatgat gctatggacg aaataagaat catgcacccg gtcggacacc ggctggggaa
     3961 aagccatgcg gattttatct aacttttcgc ggtacggtgt cgccatggat tctcctaacc
     4021 gcgcttcttg ctcaccagcg cgttaatctc aggcgtctgc acgccatctt tcaataaact
     4081 gcatgtcccc ttttcaagaa tttcctgcgc caattttttg ctccccgtga tcgccgcctt
     4141 catgaaacct gagccgtaac tcacccgcgc cacgcccaaa tcctgcaact ccttcacgct
     4201 cggcgactgc ggaccggcga gaatactgac tggggccttc acttccttgg cgatggtgcc
     4261 gatggtctcg gcggcgtgaa gattgagata gaaaatgcag tcgccacctg cctcagcgta
     4321 agcgttgcca cggcgaatcg cttcgtcgag ggctttctta ggatcattgg tcagaacatg
     4381 aaatgagtct acccgcgcat tgagaaaaaa ctcgctgccg agctcgcgct tggcgtccat
     4441 caccgctttt atcttctcta actgctgacc gagttcgacc aagccgctgc cgcctttagc
     4501 gatgctatct tcgaggttca tgcccgcaac cccggcggcg accaacgcgc gcacggtatc
     4561 ttttatgccc gaggcatcgc catagccctt ttcaccatcg gcgttgaccg gcagcgaaac
     4621 cgccgcggca aagctcgccg tcagctgcac gaacaactcg cggctcatcg cttcgccgtc
     4681 gggatacccc aacgccgcgg cgatgccgcc gctggtgcct tggatcgcct ggaagcccag
     4741 ttgttggaac agcagcgcgc tcaagacatc gtaggccccg ggcatgacga gtat
//
LOCUS       DASQKS010000548         7982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2428793, whole genome shotgun sequence.
ACCESSION   DASQKS010000548 DASQKS010000000
VERSION     DASQKS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428793"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VE170_14005"
     CDS             <1..363
                     /locus_tag="VE170_14005"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HYJ16605.1"
                     /translation="LGVFKYSEEEGTSAAKLDGKVSEDEKENRWQEVMDIQAAISRKK
                     NQDLIGTIQRVMIDGVDSDSGQLTGRTEAHAPEVDGVVYIEDSESRPFPPANAGDMLD
                     VKITGASDYDLIGETLHG"
     gene            356..814
                     /locus_tag="VE170_14010"
     CDS             356..814
                     /locus_tag="VE170_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYJ16606.1"
                     /translation="MAKADADKEKKQRRDFLKQAAEMLQDMKRSLMKEMRGRVKGETE
                     GSKDEGRDTYDLASDERDREINFILNDREREKLHAIDEALQRISEKTYGLCESCEGEI
                     QLGRLKVLPFTRLCVRCQEENEKESKRQKTLENERSYRRLDATDAEDDTF"
     gene            1082..2056
                     /gene="gluQRS"
                     /locus_tag="VE170_14015"
     CDS             1082..2056
                     /gene="gluQRS"
                     /locus_tag="VE170_14015"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HYJ16607.1"
                     /translation="MVDTEIVGRLAPSPTGYLHLGHARSFLLAWWHARSRGGRIVLRL
                     EDLDIERVKPGMLEATFEDLRWLGLDWDGEPYVQSSGVDAIDTAAAALIDQGLAYPCT
                     CTRREVQAAMSAPHQGEQTEAIYPGTCRGRYRSLAAAEQKSGRPAALRFAVPNVLVRI
                     ADGVQGVHEFDAQKEIGDFTVLRRLGMPAYQLAVVVDDARQGVTEIVRGADLLASCAR
                     QWLLQEALGYKHPRWWHVPLVTDMLGYRLAKRSDDISLARLRAAGTDPRQIVAWVARG
                     AGIDAPPRVHAEELVGRFDIARLPDSEVRVDGSDLADFGLPQNSNKST"
     gene            complement(2204..2416)
                     /locus_tag="VE170_14020"
     CDS             complement(2204..2416)
                     /locus_tag="VE170_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16608.1"
                     /translation="MIDVSLKGVRCRAAQEILDIEGTVSASTEGTIVYELEGEGGQLV
                     NVHWDDGSSSLVSSEEIVITDQVTWQ"
     gene            complement(2631..3959)
                     /locus_tag="VE170_14025"
     CDS             complement(2631..3959)
                     /locus_tag="VE170_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYJ16609.1"
                     /translation="MKRSLAQLRSDAVGIFNAAVKSADAANAVTSQVHVNDAAIEVAR
                     RVYPLANFRSVFIVGAGKAAVPMARTMEMLLGERLTGGIVVAPYGQPQMLQKVRVHEA
                     AHPIPDLAGLAGARLIAGIAGQASAHELIFVLLSGGGSALLPYPLDGLTLADKQTVTQ
                     LLLRSGATIQQINTVRRHLSELKGGKLARMADPAQVVTLILSDVIGDSLEDIASGPTA
                     PDPSRYADCLEIVRRYRLLEAIPASARSILERGSQGEIAETIKPNDPVFSRVLNVIVG
                     SNRLATEAARSYAESLGYYSTILSNTVQGESREVAKRHAASLKKAMNEPTPESICFIS
                     GGETTVTVRGAGVGGRNQEFALAAALEIAGKGGVVVLSAGTDGIDGPTYAAGALVDGA
                     TVSRGTSKGYAAAEFLLRNDSNHFLNATGDLLITGPTHTNVMDIQVMLAG"
     gene            3947..4318
                     /locus_tag="VE170_14030"
     CDS             3947..4318
                     /locus_tag="VE170_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16610.1"
                     /translation="MTVSSEPKATETDQTKQLAQLALHEKKSKCNDKEWGGTKLICRE
                     EAKTAEAILTDQLTRAGVVNIDKHVFFRMAGKEPRKNFDEIFFSLVVGVYEAFADVKL
                     VRSFAAIESDPETGAIGARLI"
     gene            complement(4469..5209)
                     /locus_tag="VE170_14035"
     CDS             complement(4469..5209)
                     /locus_tag="VE170_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ16611.1"
                     /translation="MTNANKYLALTALGFFMTGCTGLTKNQAALVGASVCGVGGGAAG
                     AATAHQGIGGKHRNEAVGAAIGAVTGALICGGLAYLMTEDPKPAPKPAPPPPPPPPPP
                     PPKPEAKPEAKPAPKPEAKPEAKPVPKPAPKVERTIILDDVLFDFDKSNVKPEAAAIL
                     DRLVAFMKENPEKKANLSGHTDNVGSDAYNQALSERRTASVKDYVVGKGVDGGRVSGQ
                     GFGESKPIADNKTAEGRAKNRRVEIKVN"
     gene            complement(5356..5763)
                     /gene="mscL"
                     /locus_tag="VE170_14040"
     CDS             complement(5356..5763)
                     /gene="mscL"
                     /locus_tag="VE170_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HYJ16612.1"
                     /translation="MIQEFKEFIQRGNVVDLAVAVVIGGAFGKIVTSFVEDVLMPPIG
                     LALGGVDFSNLFINLSGKEFASIAAAKAAGAPTLNYGLFINNIINFLIVALAIFLVVK
                     AINRLQKPAAPAPPPPPTRDQVLLTEIRDLLKK"
     gene            6085..>7982
                     /locus_tag="VE170_14045"
     CDS             6085..>7982
                     /locus_tag="VE170_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16613.1"
                     /translation="MAAVSGLAALYFWVHSDRFRQWLESEISLRTGYAVRADKLSLSL
                     PLGVVAHGVTAAKDGKTILTVTRLTATVTPLDIIANTIQRLHLERPVLQIDLTELLKS
                     KPKSTTAVALRNLRVTNGRAVIKTEDGSTIDIPAIDLTAQNINLGHESGIALRADVPQ
                     LGGSANLAIAWQSNQISLTATVMAQSNGLFRPSSAATKPVLQAQAALQTPTGKDPNVS
                     IDLRFNQFKLATKELSGQLKANAVLDKNFAAGTFTSQLDLNGFPNSVSPVVLQVSDAP
                     ATAKATGGFSITNKSIAVKSLTLHSQLGNAEAAGNLSFAPDALVTNGRVLARDLDWEM
                     VKKFMPAPFNQWNYQGRGELEMTLSGPWRALEIVGHAHSDTAQVRAANFSLSSFSLRA
                     PFRWADGQLAVQGGRIDGKKLSFAGSDTVKSAFDEIEIAAIGYDPKKPGRISAMVKIT
                     GGQFAAADSSKMGEGLALDGSVAITVQAEISSTAMVADLNVTAGELLWGSLFADLKTQ
                     KPALTIDAEYSYDKDLLQCRRCDLTLATVGRANLTGSVERITGTPQLHLQARGENLSS
                     AGFFELFLRQPFRRQYPLLDKLAISGQIDLQMQLDGSLEELAGSGNLAVKNGEIAAKS
                     NDWRIGAV"
BASE COUNT         1818 a         2311 c         2188 g         1665 t
ORIGIN      
        1 ctcggggtat tcaaatactc cgaggaagag ggcacaagcg ccgccaagct ggacggcaag
       61 gtcagcgaag atgaaaagga aaaccgctgg caggaagtga tggatatcca agccgcgatt
      121 tcgcggaaaa agaaccagga cttgattggc accatacagc gagttatgat cgacggcgtt
      181 gattcagact cgggccagct taccggccgg accgaggccc atgcaccgga ggtcgatggt
      241 gtcgtttaca tagaagactc cgaatcaaga ccgttccctc cggccaacgc tggcgatatg
      301 ctggatgtaa aaatcactgg cgcatcggat tatgacctga taggtgagac actccatggc
      361 taaagccgac gcagacaaag aaaaaaaaca gcgacgagac ttcctgaagc aagctgccga
      421 gatgcttcag gacatgaagc gctccttgat gaaggaaatg cgcgggcgag tcaagggcga
      481 gaccgaaggt tccaaggatg aaggacgcga cacctatgat ctcgccagcg atgaacgcga
      541 ccgcgaaata aacttcatcc tgaacgatcg cgaacgcgaa aagctccacg ccatcgatga
      601 agccttgcag cgtatttccg aaaagaccta cggattatgc gaaagctgcg agggggaaat
      661 ccagttgggg cggctcaaag tactgccctt cacccgtctc tgtgtccgct gccaggaaga
      721 gaacgaaaag gaaagcaagc ggcaaaagac cctggagaac gagcgcagct accgtcggct
      781 cgacgccacc gacgccgaag acgatacctt ttaattaaac acccgagatc tccgcaaccg
      841 gccaagccat tatcaacaac cgctcagtga tttcaccccg cgcaacttaa ctcaacagcg
      901 gcggagttca cagtcaaatt actctggtcg gggcggcatg cgtcacggcc gctcacggca
      961 aatctcggcg tacgagtttt ttgtcaggcc tgtccaattt gcctgaccta tttctcactt
     1021 ttatagcgtt tggtattttg accgtcattg atcctttggg cccaacggac gtttccgcaa
     1081 tatggtggac acagagatcg tcggccgctt ggcacccagt cccaccggct acttgcacct
     1141 gggacacgct cgctcttttc tcctggcctg gtggcatgcg cgttcgcgcg gcggccgtat
     1201 cgtgctgcgc ttggaggatc ttgacatcga gcgcgtaaag ccgggcatgc tcgaagcgac
     1261 gttcgaggat ttgcgctggc tcggcctcga ctgggatggc gagccctatg tccagtcgag
     1321 cggtgtcgat gcaatcgata ccgcagccgc agccctgatc gatcagggcc tcgcctatcc
     1381 ctgcacctgc acacgtagag aggttcaagc ggctatgtcc gccccccacc agggcgaaca
     1441 gaccgaagcg atttatccgg gcacttgtcg tggccggtat cgaagtttgg ccgcggccga
     1501 acagaaatcc ggccggcctg cggccttgcg cttcgctgtg ccgaatgtgc tcgtgcgcat
     1561 cgcagacggc gttcagggtg tgcatgagtt cgacgcgcag aaagagatcg gcgactttac
     1621 cgttctccgc cgcctcggca tgccggcata ccaactcgca gtcgtcgttg acgacgcccg
     1681 ccaaggcgtc acagagatcg ttcgtggtgc cgatctgctg gccagctgcg cccgccagtg
     1741 gttattgcag gaagcgctgg gctacaaaca tccacgatgg tggcatgtgc cgctcgtcac
     1801 cgacatgttg gggtaccgtc tggccaagcg cagcgacgat atatcgctcg cccgcctgcg
     1861 cgccgctggc accgatccgc ggcaaatcgt cgcgtgggtt gcgcgcggcg cgggcattga
     1921 cgcaccgcca cgggttcacg ccgaagagct agttggtcgt ttcgacatag cgcgcttgcc
     1981 cgattccgag gttcgcgtcg atgggtcgga tcttgcggat ttcggtctgc cgcaaaattc
     2041 aaacaaatct acgtaacagt aaatgccggc gcgggccaag tttgtggaag tcacccggtg
     2101 gtttggggag tctcgagtga atggctttag ataaaattgt cttgggaccc gcgctcgagg
     2161 cagggagaca aaatcgccag ttgatttgac aattaactca actttattgc caagtgactt
     2221 gatccgtgat gacgatctct tcggacgaca ccagcgagct gctgccatcg tcccaatgca
     2281 cgttgaccag ttgtccgcct tcgccttcta actcgtaaac aatcgtgccc tcggtcgagg
     2341 cactcaccgt cccttcgata tcgaggatct cctgcgcggc gcggcagcga actcccttca
     2401 agcttacgtc aatcatcttc ctgctcccaa acagtttcca ggccaaggac tccgaaaaat
     2461 ggcactctaa tcatgaacga atttccaaat caatagccca ttggcgagaa atttcgccac
     2521 cgcgttaggc agcctgagcc cgttaaggga atgttacgaa agatttttgg cttcgacagg
     2581 cgcggccgtg acggctggtc tcgctcagct aacttggcgt ttcgtatcac ttaccccgcc
     2641 aacatcacct gaatatccat gacattcgtg tgggttggtc cagtgatcag cagatcaccg
     2701 gtcgcattta ggaaatggtt gctgtcgttg cgcagcaaaa attccgcggc ggcgtaacct
     2761 ttcgacgtgc cgcgcgaaac cgtcgcgccg tcgacgagag caccggcggc gtacgtcggc
     2821 ccgtcgatac catcggttcc ggcgctgagc acgaccactc ctcccttgcc ggcgatctcg
     2881 agagccgcgg caagagcgaa ctcttgattg cgccccccca cgccagcgcc gcgtacggta
     2941 acggtggtct caccgccgga gatgaagcat atggattccg gcgtcggttc attcatggct
     3001 ttcttcagcg atgcagcgtg tcgcttggca acctcgcggc tttcgccttg gacggtattt
     3061 gacaaaatcg tcgagtaata acccaagctt tccgcgtagg atcgcgccgc ttcggttgcg
     3121 aggcggttgc tgccgacgat tacgttgagc acccgagaga aaactggatc gttgggttta
     3181 attgtttcag caatctcacc ttggctgccg cgctcgagaa ttgatcgcgc tgatgctgga
     3241 atagcctcca gcagccgata ccttcgaaca atctccagac agtcggcgta tcggctcggg
     3301 tccggcgccg tcggcccaga agcgatatcc tcaaggctat cgccgatgac atcggacagg
     3361 atcaaggtga ccacctgagc gggatcggcc atgcgggcga gcttgccgcc cttgagctcc
     3421 gacagatgac ggcgcacggt attgatttgt tgaatggttg cgccgctgcg caagagcaac
     3481 tgcgtgactg tttgcttatc cgccaacgtc aaaccgtcaa gcggataggg caacagcgct
     3541 gaaccgccac ccgatagtag gacaaaaatt agctcgtgcg cggacgcctg acccgcgatg
     3601 ccagcgatga gacgcgcacc tgccaaaccg gcaagatcag gaatcggatg cgcggcttcg
     3661 tgaacacgaa ctttttgcag catctgcggt tggccatatg gggccacgac gatgccgccg
     3721 gtgagccgct cgccaagcaa catctccata gtccgcgcca ttggcacggc agctttgccc
     3781 gcgccgacga taaagacact gcgaaaattt gccaaggggt aaactcgacg cgcgacttcg
     3841 atcgctgcat cgtttacatg aacctgactt gtgacagcat tggccgcgtc agcggacttt
     3901 accgcagcat tgaagatacc gacagcatcc gagcgcagct gtgccaatga ccgtttcatc
     3961 tgagccgaaa gctactgaaa cagaccaaac aaaacaactc gctcaattag cgctgcatga
     4021 gaaaaaatcc aaatgcaatg ataaagaatg ggggggaacg aaactaattt gccgggaaga
     4081 ggcgaaaacc gccgaagcca tactcaccga tcagcttact cgtgctggtg tcgtaaatat
     4141 cgataagcat gtttttttcc ggatggccgg caaggaaccg cgaaaaaatt tcgatgaaat
     4201 tttctttagc ctcgtcgtcg gcgtttatga agccttcgcc gacgtaaagc ttgtgcggtc
     4261 gtttgctgct atcgagtctg atccagaaac gggtgccatt ggagcccgct tgatctagca
     4321 aagtacgaaa ttcgcggacc ttggcttcaa tctctttaaa gttaaaggcc tgggcatcgg
     4381 ccaatgcagg aaaccaaaac gcggatgcca ggaagagcgc acttagtgtc tttcctggca
     4441 tccgcatgat gcaaaacgtt agcaaaatct agttgacctt gatttcgacc cggcggttct
     4501 tggcgcgacc ctccgcggtc ttgttatccg cgatcggctt gctctcgccg aaaccctgcc
     4561 ccgaaacccg accaccgtcg acacccttgc cgacgacata gtccttaacc gaggctgtcc
     4621 gtctctcgga aagcgcctgg ttataagcgt cactgccgac gttatcggta tgaccggaaa
     4681 ggttggcctt cttctctggg ttctctttca tgaaagcgac taatcgatcc aagattgccg
     4741 ccgcttccgg cttcacattg ctcttgtcga agtcgaagag cacgtcgtca agtatgatcg
     4801 tccgctcaac ctttggcgcc ggcttcggaa ccggttttgc ttccggtttt gcctccggtt
     4861 tcggcgctgg tttcgcttct ggtttcgctt ccggttttgg cggcggagga ggtggaggtg
     4921 gaggaggcgg cggagctggc ttcggtgccg gcttcggatc ctcggtcatt aaataagcta
     4981 gaccaccaca aatcagcgcg ccggtaactg cgccgatcgc cgcaccgacc gcttcattgc
     5041 ggtgctttcc gccaatcccc tgatgcgccg tagcagcgcc agcggctccg ccgcccacac
     5101 cgcagaccga agcacccact aacgctgctt gattcttggt taaaccggta cacccggtca
     5161 taaagaaacc taacgcggtt agcgctagat atttgtttgc gttcgtcatt tctgcctccc
     5221 atgcgaaaaa tccgaaattt tggccaaatt agtataaggg tcgtaagttg tcaagaaaaa
     5281 accccaattc tttaaaacgc cgtgcttgac ctatcgtttg cttcaaagtc tggtccggta
     5341 actgtgcgct agactttact tctttaaaag gtcgcggatt tccgtcaaca atacctgatc
     5401 cctggtcggc gggggtgggg gcgctggcgc tgccggcttt tgcaatcgat tgatcgcctt
     5461 gaccaccaga aatattgcca aggcgacaat caagaaattg atgatgttgt taataaacaa
     5521 tccatagttc aaggttggcg cgccggccgc cttggccgcc gcaatgctcg cgaattcttt
     5581 tcctgacaaa ttgataaaca agttggagaa atcgacgccg ccaagcgcca aaccgatggg
     5641 cggcatcagc acatcttcga cgaaagaagt gacgatcttg ccgaaggcgc cgccgatgac
     5701 cacggcgacg gcaagatcca cgacgttgcc cctttgaata aactctttaa actcttgaat
     5761 catgactagc tcctcctaaa agagaattcg ctccgcttcc cttggaccat gataaattcc
     5821 ttagagaatg agcccccttt tgaaccgcaa gcctatctga ttcctggctg gttccttata
     5881 ggacagagaa aaactctctg tcaaattgca aaacggattt ctgtcatgaa caatcaatct
     5941 ggtacacgag catattcgtg ttttcacgcc gactcggacg cgagctgagg ctcagacgca
     6001 gttgattcac cggttcgtca gcgttggcag cccatgatga caaaccccgc ccgcagaatt
     6061 gttaaagcac tttgggtcgt ttccgtggcc gccgttagcg gcctcgccgc tctctatttt
     6121 tgggtgcatt ctgatcgctt tcgccagtgg ctggagagcg aaatttcgct gcgcaccggg
     6181 tacgccgtgc gcgccgacaa actgagctta tcgctgccgc tcggcgtggt tgcccacggc
     6241 gttaccgccg caaaggacgg taagacgata ttgaccgtga ccagactgac cgccaccgtc
     6301 acgccgctgg atatcattgc aaataccatc cagcgcctcc acctggagcg gccagtattg
     6361 cagatcgacc tgaccgaact gctcaagtcg aaaccaaaaa gcacaactgc cgtggctctg
     6421 cgtaacttgc gcgtgaccaa cggaagagcg gtcatcaaga ccgaagatgg cagcacaatc
     6481 gacatcccgg cgatcgatct aaccgcgcag aacattaatt tgggccatga aagcggcatc
     6541 gccctgcgcg ccgatgtgcc gcagcttggt ggcagcgcca atcttgcgat cgcatggcag
     6601 tccaaccaga tttcgctgac ggccacggtc atggcgcaaa gcaatggcct atttcgtccg
     6661 agtagtgccg cgaccaaacc ggttcttcaa gcccaagccg cgcttcagac acccactgga
     6721 aaagatccga atgtaagtat cgatctgcga tttaaccagt tcaaactagc gacaaaagaa
     6781 ctgtccggcc agcttaaagc caacgccgtg ctagacaaaa atttcgccgc cggcacgttc
     6841 acaagccagc tggatctcaa tggcttcccc aacagtgtta gtccggtggt actgcaagtt
     6901 agcgacgcac cggcgacggc aaaggccacc ggcggattct ccatcaccaa caaatccatc
     6961 gccgtcaaat cacttacact gcattcgcaa ctgggcaatg ccgaggccgc aggcaatctg
     7021 tcgttcgcac cggatgcgct ggtcaccaac ggccgcgtgc ttgcccgcga tctcgactgg
     7081 gagatggtga aaaagttcat gccggcgccg ttcaatcaat ggaactatca aggccggggt
     7141 gagttggaaa tgaccctaag cggcccatgg cgggcgctcg aaatcgtcgg tcatgcccac
     7201 agcgacaccg cccaggtgcg cgcggcgaat ttttctctgt ccagcttttc gctaagagcg
     7261 ccgtttcgct gggccgatgg ccaactggcg gtacagggcg gccgcatcga tggcaagaag
     7321 ctctcgttcg ctggcagtga cacagtcaaa agcgccttcg acgagatcga aatcgccgcc
     7381 attggctacg atcccaagaa accggggcgg atcagtgcga tggtgaagat taccgggggg
     7441 cagttcgccg ctgccgacag cagtaaaatg ggcgaaggct tggccctaga cggcagcgtc
     7501 gcgataaccg tccaagccga aataagttcc acggcgatgg tcgccgatct caatgtcacc
     7561 gctggagaac tgttatgggg cagcttattc gccgacctaa aaactcaaaa accggcgctg
     7621 acaatcgacg ccgaatattc gtatgacaag gacctcctgc aatgccgccg ctgcgatttg
     7681 acgctcgcga ccgtcggccg ggcaaatctc accggcagcg tcgagcggat aaccgggacg
     7741 ccgcagttgc acctgcaagc ccgcggcgaa aacctttcgt ccgcgggttt ttttgaatta
     7801 ttcctgcgcc aacctttccg ccggcaatat ccgctgctcg acaagctcgc gattagcggc
     7861 cagatcgact tgcaaatgca gttggacggc tccttggagg agctcgccgg cagcggcaac
     7921 ctagctgtga agaacggcga gatcgccgcg aaatcgaacg attggcggat cggcgcggtg
     7981 aa
//
LOCUS       DASQKS010000549         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1158366, whole genome shotgun sequence.
ACCESSION   DASQKS010000549 DASQKS010000000
VERSION     DASQKS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158366"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VE170_14050"
     CDS             <1..530
                     /locus_tag="VE170_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HYJ16614.1"
                     /translation="PKVESAKTSLTALNPEVEVIVHPVRFHETNAEEIAKGSDFIVDG
                     SDNFDTKFLVNDLAVKLSIPFSHAGIVRLQGQVMTVVPGKSACYRCLFKVPPPAEEIL
                     NCQVSGILGAVAGTIGTIQATEAVKYLAGFEDGLLTDRLLIYDAKNMRFREIEVRRDP
                     QCAACGTTADKSATA"
     gene            812..1090
                     /locus_tag="VE170_14055"
     CDS             812..1090
                     /locus_tag="VE170_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HYJ16615.1"
                     /translation="MPVRVRVPTPLRKYTQGADEVGAEGGNVKSLVEDLEKNYPGIKE
                     RICDDTGKVRRFVNVYVNGDDIRFLQNLDTALKEGDNISIVPAIAGGC"
     gene            1114..2115
                     /locus_tag="VE170_14060"
     CDS             1114..2115
                     /locus_tag="VE170_14060"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HYJ16616.1"
                     /translation="MSQTSPNLRLIPANPGAPNKVESTLDLIGNTPLLEIQRIGAGLA
                     KGVRVFAKLEGFNPGGSVKDRPALRMIQEGLRTQKLRPGKTIIDSTSGNTGIALAMIG
                     AILGYPVELAIPANVSVERKQIINAYGAKMIFSDPMEGSDGAIRLCRKLLDADPEKYF
                     KPDQYFNPMNSQAHYENTGPEIYRQTNHAISHFVAGIGTGGTIMGTGRYLKEVNRSIQ
                     VIAVEPDDALHGLEGLKHMASSIVPGIYHEEELDSKIPVSTEDAYAMVYRLSQEEGVL
                     VGQSSGAALFAALQIARKLRSGTVVTLFPDFGDKYLTTNLWVGWRDRAAVGNLKFSI"
     gene            complement(2393..2857)
                     /locus_tag="VE170_14065"
     CDS             complement(2393..2857)
                     /locus_tag="VE170_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16617.1"
                     /translation="MPKKLQKPVLLFSLAFLAVAGWLHGADKAPQSVQVTIRTILAGD
                     KSDEFDPKLKGMEQQLKPLKHRSYRALKEDSQNIPWHGSRAFEIPGGRALTIAPQEFQ
                     DNRIGLKIRLTHGDKPLIDTTVKIANKGNIILGGPAHEGGSLVLSISAAVTH"
     gene            complement(2820..3350)
                     /locus_tag="VE170_14070"
     CDS             complement(2820..3350)
                     /locus_tag="VE170_14070"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYJ16618.1"
                     /translation="MNYCESLQESIGRWLDGELPPAESEAVRSHVASCAECSAARQRL
                     EKIQLTLGTMLTAEAARLEFAPFWREVRHRIEAKRPWHEELIETVQTLFSVSRLAWAV
                     PVAIMLVLGFLSLTKWPGGGETSNYASVESIDAHGRNVALLREDKTKTTIIWLYQDPE
                     GEDENAEETTKTGPAF"
     gene            complement(3347..>3817)
                     /locus_tag="VE170_14075"
     CDS             complement(3347..>3817)
                     /locus_tag="VE170_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ16619.1"
                     /translation="QKVFMVILGLMRNREDALEVAQETFFRAFRKIKSFQGGSSFYTW
                     IYRIAVNNAIDAQRKQKRNPLDFYETMDELFAARNEVAKDPFADVHDRQLRENMAQAI
                     NELTPEHKAVIVLRTIEGMSYRDIGEILGCSEGTVMSRLHYARKKLQDKLKAFL"
BASE COUNT          870 a         1085 c         1008 g          854 t
ORIGIN      
        1 ggcccaaagt cgagtcggcg aaaacaagct tgaccgccct caaccccgaa gtcgaagtga
       61 tcgtccatcc ggttcgcttc catgagacca atgccgagga aatcgccaag ggctccgact
      121 tcatcgtcga cggcagcgat aactttgaca ccaagtttct cgtcaacgac ctggcggtga
      181 aattgagcat ccctttctct catgccggca tcgtccgctt gcagggccaa gtcatgaccg
      241 tggtgcctgg caagagtgcg tgctaccgct gtctgttcaa agtcccgcca cccgctgaag
      301 aaatcttaaa ctgccaagtc tccggcattc tcggcgctgt cgccggcacc attggcacca
      361 tccaggccac cgaagcagtg aaatatttgg ccggcttcga ggacggcctg ctcaccgatc
      421 gcttgttgat ctacgacgcc aagaatatgc gcttccgcga gatcgaagtc aggcgggacc
      481 cgcaatgcgc cgcctgcggc acgaccgctg acaagagcgc aaccgcttag ctgccatcag
      541 attaattgat cattgatcgg accagccacc ggcagtgcct taacctagcg caattgacca
      601 ctgtaatgta tcgacatcgt gacagccgta aagccaatac aatttgagat agttcgagag
      661 ctattgctag cgtggcattc agccggcgaa gccgtatggc tggcgcctaa cccgccggta
      721 gggttgcgac tccttcatct tgaaaggcat agaatgaagg actattgtcg gattgttcag
      781 taatttactt gaaactttca ggaggttagt catgcccgtg cgagttcgag ttcccacccc
      841 gctgcgtaag tatacgcaag gggccgacga agtgggtgcc gaaggcggca acgtcaagtc
      901 tctggtcgaa gatttggaaa aaaactatcc cggcatcaaa gagcgcatct gcgacgacac
      961 cggcaaggtg cgccgcttcg tcaacgttta tgtgaacggg gacgatattc gttttttgca
     1021 aaatctcgac acggcgctca aggaaggcga taacatttcc atcgtgcccg ccattgccgg
     1081 cggctgttag tcgaccgacc ttttatagcg acgatgagcc aaacctcgcc caacttgcgc
     1141 ctcattccag cgaacccggg cgcgccgaat aaagtcgaat cgaccctcga tttgatcggc
     1201 aacaccccgc tcttggaaat tcagcggatc ggtgcaggct tagccaaggg cgtcagagtt
     1261 tttgccaaac tggaaggctt caatccgggc ggctcggtta aagaccgtcc agcattgcgc
     1321 atgattcagg aagggctgcg cacacagaag ctgcgaccgg gaaaaaccat catcgactca
     1381 acctcgggga ataccggcat cgccctggcg atgatcggcg ccattcttgg ctatccggtg
     1441 gagctggcga tccccgccaa tgtcagcgtc gagcgcaagc aaatcatcaa cgcctacggc
     1501 gccaagatga tttttagcga tccgatggag ggctccgatg gcgctatccg tttgtgccgt
     1561 aagcttctcg atgccgatcc ggaaaaatat tttaaaccgg atcagtactt caatccgatg
     1621 aattcccaag ctcactacga aaataccgga ccggagattt accgccaaac caatcacgcg
     1681 atcagccatt tcgtcgccgg cattggcacc ggcggcacga tcatgggcac cggccgctat
     1741 ttgaaagagg ttaatcgatc gattcaagtg atcgccgtcg agccggacga tgcattacac
     1801 ggtctcgaag ggttgaagca tatggccagt tccatcgtgc cggggattta tcacgaggaa
     1861 gaactcgaca gcaagattcc ggtatccacc gaggacgcct acgcgatggt gtatcgtttg
     1921 agccaagaag aaggcgtgct ggtcggccaa tcctcgggcg ctgcgctgtt cgccgcgctg
     1981 caaatcgcgc gcaaacttcg ctctggaacc gtggtcacgc tcttccccga ttttggcgac
     2041 aagtacctga ctaccaatct atgggtcggc tggcgcgacc gcgccgcggt gggcaacctt
     2101 aaattttcca tttgacccga cggcaaagaa agccaagacc gattccttgc gagagtcgca
     2161 tcgatctcac cttagccaaa gactccgtct aagacggatt tacgcctagt aagaaaaaca
     2221 gttaccaacg tgattttaat attgcccgca gttccacgat gactgcggat ctgcagctgt
     2281 tcgcgtcggc aatagatgga aatgagatcg atgtcgccgc cgatcactgg ctcaacggac
     2341 aggggatggc ttttccagcc cgtggcgaga aaagtaatcg atgggcgaat ttttagtgcg
     2401 tgacggcggc tgagatcgac aaaaccaatg atccgccctc atgcgccggt ccgccgagaa
     2461 taatattccc cttgttggca atcttgaccg tggtgtcgat cagaggcttg tcaccatggg
     2521 tcaatcgaat cttcaaacct atgcgattat cctggaactc ctggggagcg atcgtcagcg
     2581 cgcgtccacc ggggatttca aacgccctgc tgccgtgcca agggatgttt tggctgtcct
     2641 ctttgagtgc tcggtaggaa cggtgcttca agggtttgag ttgctgctcc atgcctttga
     2701 gcttgggatc gaactcatcg cttttatcgc ctgccaagat cgttcggata gtcacttgta
     2761 cggattgagg agccttatcc gcgccgtgta gccagccggc gacggctaga aaagccaggc
     2821 taaaaagcag gaccggtttt tgtagtttct tcggcattct cgtcttcacc ttcgggatct
     2881 tgataaagcc aaataattgt cgtcttggtt ttgtcctcgc gtaagagggc gacgttgcgg
     2941 ccatgggcat cgatggactc caccgatgca tagttgctgg tctcgccgcc accgggccat
     3001 ttggttagtg ataagaaacc cagcaccagc atgatcgcca ccggcactgc ccaagcgaga
     3061 cgcgatacac taaacaatgt ctgtaccgtt tcgatcaact cctcatgcca gggccgtttc
     3121 gcttcaatcc ggtgccgcac ttcacgccaa aatggcgcga attcgagccg cgccgcttct
     3181 gccgtaagca tggtgccgag ggtgagctgg attttttcca agcgctggcg cgccgcgctg
     3241 cactcggcgc aactggccac gtggctgcgc accgcttcgc tttccgcagg tggcagctcg
     3301 ccgtccagcc agcgaccgat ggattcctgc aaagattcac agtaattcat aaaaaggctt
     3361 tcaacttatc ctgtagtttt tttctcgcat agtgtaaccg actcatgacc gttccctcag
     3421 aacaacccag aatctcgcca atatcccgat aggacatgcc ttcaatggtg cgcaagacga
     3481 ttacggcttt gtgctccggt gtcaattcgt tgatcgcctg cgccatgttc tcccgcagct
     3541 ggcgatcatg aacatcggcg aacggatcct tggctacctc gttgcgcgcc gcgaacagct
     3601 catccatggt ctcgtagaag tccaacggat tgcgtttctg cttgcgttgg gcatcgatcg
     3661 cattgttcac cgcgatccga taaatccagg tataaaacga cgagccaccc tgaaagcttt
     3721 tgattttgcg aaatgcccta aaaaaagttt cctgggccac ttccaacgca tcctcgcggt
     3781 tgcgcatcag cccgagaatt accatgaaaa ccttttg
//
LOCUS       DASQKS010000550         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2429157, whole genome shotgun sequence.
ACCESSION   DASQKS010000550 DASQKS010000000
VERSION     DASQKS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429157"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VE170_14080"
     CDS             <1..1006
                     /locus_tag="VE170_14080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16620.1"
                     /translation="LVLSSFAHSLGGLYFSYGIVTALGQGALSFVGHNALISFWFVRK
                     RATAIGIASMGQGLGALVMVPATQLLVSSIGWRWTYVATGALLLLILVPANAFFQRRT
                     PAEVGQFPDGDAAPVSGAGAGRHGAKHAGQRDWTLGQAVRSFPFWCITIGHLALGTAL
                     FMINTHAIAHFVAVGYEKLAASFYFGFIGFIRIGATLIWGSLSDRLGRSQTYAVATAV
                     TALGVAGMIAMTFGAPLWLVYLTIALYGIGHSAGNPTYGSVIGDIFSGRKIGLIFGFL
                     EISFGFGSAFGSWIGGYLFDTTGSYAWSFGVCLLCYIISGLAIHLCVRWHSREMARAH
                     "
     gene            1268..2260
                     /locus_tag="VE170_14085"
     CDS             1268..2260
                     /locus_tag="VE170_14085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16621.1"
                     /translation="MNFCLHGSARTVNEAVERAKRAEALGFEAIFFADSQMNNVDPYQ
                     AMALCALNTKTIRFGTAVTNMVYRDPTITACSYATLNEISGGRAIVGMGTGDGPVYSL
                     GRTATKLVDFEKGLRVIRDLLHDRGIDIPKSHERAGGVVKLKAGKRPVPIYISAEGPK
                     TLRVAGRICDGIILGTGFDKQVTDWARQQLAEGANNEGRTLADIDIMPAGMIVVDDDG
                     DLARRRVRSRMANRAHHNFRFTMETVPEGEAAGIQKFMDNFDISKPIEERVDPGLVTD
                     YLLERFTIAGTSEECIAKVKSLEADGIKRILLTPPNAIYDQVMDKWGRQVIEKY"
     gene            2643..3299
                     /locus_tag="VE170_14090"
     CDS             2643..3299
                     /locus_tag="VE170_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ16622.1"
                     /translation="MHVKFTTWIAAAKISLSLMMFMFAIASSVTAAEKAKNDLVVKDG
                     MLVSMDYTLKGTDGKLIDTNKGREPLKYVHGQKMMIPGLERELTGMKVGGEKNVTVKP
                     EDAYGPINKNAFQEVPKEKLPANGLKVGAVLAAKSPEGQVVPMRVHEIKDSTVVMDMN
                     HPMAGKTLVFNVKIVDIQPAPPPPPPTAAKPVAPAKPSEPAKPATPAQPAKPADPTAK
                     "
     gene            3319..4068
                     /locus_tag="VE170_14095"
     CDS             3319..4068
                     /locus_tag="VE170_14095"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HYJ16623.1"
                     /translation="MELEEFAARVRESQELVFFTGAGISTESGVPDFRSPGGVWTKYQ
                     PVLFQDFLASESARVQHWRLKKATYELFKTVAPNSGHQAICQFEQRGQLLGLITQNID
                     GLHKLAGTSDSRLVELHGSDRLVTCLKCGKAFQPADVYEKWHDGLPTPTCEGCGGFLK
                     SANVSFGQSMPVEAMQRAQRWSEQADIFVVIGSSLQVQPAASFPVIAKRSGALLAIVN
                     RDPTPLDDLADFVHNGAIGAFFTELKIRLDH"
     gene            complement(4065..>4306)
                     /locus_tag="VE170_14100"
     CDS             complement(4065..>4306)
                     /locus_tag="VE170_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16624.1"
                     /translation="TTINRRGLWLARLAGIGAEPVGKDGFDVCHSVSGQRLRRWFTIR
                     LVDESFNSSEIVRLEPMSFTVISRNGFVEYGAGAI"
BASE COUNT          956 a         1335 c         1160 g          855 t
ORIGIN      
        1 actagtgctg tcgtctttcg cccactcgct cggcggccta tatttttctt acggcatcgt
       61 caccgccttg ggccagggcg ccctcagttt tgtcggccat aatgcgttga tctcattctg
      121 gttcgtgcgc aagcgtgcca ccgccatcgg catcgcatcc atgggccaag gcttgggcgc
      181 gctggtgatg gtgccggcga cgcaattgct ggtcagttcc atcggctggc gttggaccta
      241 cgtcgccacc ggcgctttac ttttgctcat attagttccg gccaacgcat tctttcagcg
      301 ccgcacgccg gcggaggtcg gccagtttcc cgacggcgac gccgcgccgg ttagcggcgc
      361 cggcgccggg cggcatggcg cgaaacacgc aggccagcgc gactggaccc tcggccaagc
      421 ggtacggtca tttccattct ggtgcatcac catcggccac ttggcgctgg gtaccgcact
      481 gttcatgatc aacacccacg ccatcgccca tttcgtcgcc gtcggttatg aaaagttagc
      541 cgcctctttt tacttcggct tcattggttt catccgaatt ggtgcgacgc tgatatgggg
      601 ttcgctctcc gaccgcctcg gccgcagcca aacctatgct gtcgccaccg ccgtcaccgc
      661 gctcggcgtc gccggcatga tcgccatgac cttcggcgca ccgctctggc tggtctatct
      721 caccatcgct ctctacggca tcggccacag cgccggcaac ccaacctacg gttcggtcat
      781 cggcgacatt ttttccggcc gcaagatcgg cctgattttc ggatttttgg aaatcagctt
      841 tggcttcggc agtgccttcg gctcctggat cggcggctat ctgttcgaca ccaccggaag
      901 ttatgcctgg tcgtttggcg tctgtctcct ctgctacatc atctccggcc tggcgattca
      961 cctctgcgtc cgctggcaca gccgggaaat ggcgcgtgcc cattgacacc gccgaataca
     1021 cccgcctaat aaatgttggg aacggattgg atttcaccac aacgacctgc gcggaatagt
     1081 cgcaacctaa attcggaata tttcgcacaa agggcgcaaa ggaacagcgt tgtcatttcg
     1141 aacggagaga gaaatccttc ttaaatccct cgccttcgct cgagatgaca ggcctctggt
     1201 ctgtcacttt acgaccctgg agtccttggc gcgcagaata tccgaatctg aaacctaagg
     1261 agcaatcatg aacttctgtc tacatggttc ggcgcgcacg gtgaacgaag ccgtagagcg
     1321 cgccaaacgc gccgaagcgc tcggcttcga ggcgatcttt ttcgccgaca gtcagatgaa
     1381 caacgtcgac ccctaccaag cgatggccct gtgcgccctc aacacgaaaa ccatccgctt
     1441 cggcaccgct gtgaccaaca tggtctaccg cgatccgacc atcacagcgt gctcctacgc
     1501 gacgctgaac gaaatttccg gcggccgcgc catcgtcggc atgggcaccg gtgatgggcc
     1561 ggtctatagc ctgggtcgga ccgcgacgaa gctcgtcgac ttcgagaaag gattgcgcgt
     1621 gatccgcgac ctgctccatg accgcggcat cgatattcca aagagccatg agcgagccgg
     1681 cggtgtggtc aaattaaaag ccggcaagag accggtgccg atttatatct cggcggaagg
     1741 accgaaaacc ctgcgggtcg ccggccgcat ctgcgatgga atcatcttgg gaaccggctt
     1801 cgacaagcag gtcaccgact gggcgcgcca acaactcgcc gaaggcgcca ataacgaagg
     1861 gcgaacatta gccgacatcg acatcatgcc cgccggcatg atcgtcgtcg atgacgacgg
     1921 cgatctggcg cgccggcgtg tgcgcagccg catggccaac cgcgctcatc ataattttcg
     1981 tttcaccatg gaaaccgtgc ccgaaggcga ggccgctggc attcaaaaat tcatggacaa
     2041 tttcgacatc tctaaaccga tcgaagagcg cgtcgacccc ggcttggtca ccgattattt
     2101 actcgaacgc ttcaccatag ccggcacgtc cgaggaatgc atcgccaaag tgaaaagcct
     2161 cgaagccgac gggatcaagc gaatactcct gacaccgccc aacgcgatct acgatcaagt
     2221 catggataag tggggcaggc aggtaatcga aaaatattag caggctgctg aaaacaggcc
     2281 catagactcc gttgcgctcg atcgtcgcgc gtcaaccagc ctgtcctgag ccgagtcgaa
     2341 gaatcgattt ttcgtgtgcc ttgctctgga tcttttgcca cagcctgcaa aagatttctt
     2401 cagcaacctg ctagaggtca aaagtcgagg gcaattattc catgcgatca cctagcctca
     2461 atcctcgatc ctcgactttg ctactgcttg ctgcctcgaa atgaccaaac agcggaactc
     2521 accataggga acgagaataa atatcctgcg ggattgaaac cgaagattcg ttgagttcac
     2581 cattctcacc atgtactctt aataggaatc cgatcgcagt aaatgcttga gaggggaatt
     2641 acatgcacgt taaatttacc acttggatcg ccgccgccaa gatttcgctg tcgctgatga
     2701 tgttcatgtt cgctatcgca tcatcggtca ccgcggcgga gaaagccaag aacgatctgg
     2761 tcgtcaaaga cggcatgctg gtgagcatgg attacacgct caagggcact gacggcaaac
     2821 tcatcgacac taacaagggt cgggaacctc tcaagtatgt ccacgggcaa aagatgatga
     2881 tccccggttt ggagagggag ctcacaggca tgaaggtcgg cggcgagaag aacgtcacgg
     2941 tcaaacccga agacgcctat gggccgatta ataagaatgc ctttcaagaa gtcccgaaag
     3001 aaaaactgcc cgccaatggt ttgaaagtcg gcgcggtatt ggcggcgaag agtcccgagg
     3061 ggcaagtcgt acccatgcgc gtccatgaaa tcaaagacag cacggtggtc atggacatga
     3121 accatccgat ggccggcaag acgctagtct tcaacgtcaa gatcgtcgac atccagccgg
     3181 ctccgccgcc gccgccgcca acagctgcca agccagtggc accggcgaaa ccttcggagc
     3241 cggcaaaacc ggcaacaccg gcgcaaccgg caaaacccgc ggaccctacg gcaaaataac
     3301 cggacgagtc acccgttcat ggaactcgaa gaattcgccg cccgagtgcg tgagtcgcaa
     3361 gagctggttt ttttcaccgg cgccggcatc agcaccgaat ccggcgtgcc cgacttccgc
     3421 agccccggcg gcgtctggac aaaataccag ccggtgctgt tccaagactt tctcgccagc
     3481 gaatccgcgc gggtgcagca ctggcggctc aaaaaagcca cctacgaact gttcaaaacc
     3541 gtcgcgccca acagcggcca tcaggcgata tgtcaattcg aacagcgcgg tcagttgctc
     3601 ggcctgatca cgcaaaatat cgatggcctg cacaagctcg ccggcacttc ggacagccgt
     3661 ttggtcgagc tgcacggcag cgatcgtttg gtcacctgcc tcaagtgcgg caaggctttt
     3721 caacctgccg acgtctatga aaaatggcat gacggactgc cgacgccgac ttgcgaggga
     3781 tgcggcggtt tcttgaaatc cgccaacgtc tccttcggcc aatcgatgcc cgtggaagcg
     3841 atgcagcgcg cccagcgctg gagcgagcaa gcagatatct tcgtcgtcat cggctcatcc
     3901 ctgcaagtgc aaccggcggc atccttcccg gtcatcgcca aacgcagcgg cgcgctcctc
     3961 gccatcgtca accgcgaccc gacgcccctc gacgacctcg ccgacttcgt tcataacggc
     4021 gccatcggcg cattctttac cgagctcaag attcggctcg atcattagat cgccccggca
     4081 ccatattcaa caaacccgtt gcgagagatc accgtgaagc tcatgggctc taagcggacg
     4141 atctcactcg agttaaagct ttcgtcgacg aggcggatag taaaccaccg ccgcaagcgc
     4201 tgcccactga cagaatggca aacatcgaag ccatccttac ctactggttc ggcgccaatc
     4261 ccggcaagcc gcgctagcca aagaccgcgc cgatttatgg tggtcg
//
LOCUS       DASQKS010000551         7077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5348562, whole genome shotgun sequence.
ACCESSION   DASQKS010000551 DASQKS010000000
VERSION     DASQKS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348562"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VE170_14105"
     CDS             <1..276
                     /locus_tag="VE170_14105"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311478.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYJ16625.1"
                     /translation="LDYPGVGPELSYLRDQARVQFDAATDEEAIASIRLLAEIEGIIP
                     ALESAHAIAVVRRLAPKLKKRQIVIVNLSGRGDKDMITVGDHLGVKL"
     gene            374..1186
                     /gene="trpA"
                     /locus_tag="VE170_14110"
     CDS             374..1186
                     /gene="trpA"
                     /locus_tag="VE170_14110"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYJ16626.1"
                     /translation="MSRIEKKFIAVKRRGEAALVPFITAGDPDLATTLKIMRALETGG
                     ADAIELGIPFSDPSADGPTIQRSSERALKNPISLPAIFRLVRDFRRTSELPIILFGYY
                     NPFFRFGLDKFATQAAQAGADGVLCVDLPPEECGELKSCTDRCDLDIIFLLSPTSGED
                     RRRLVARHGRGFIYYVSVTGVTGARQSFATDLRRQVAQVRKVTSLPVGVGFGISTPKQ
                     AAAIAQFADAVVVGSALVQRIEQGKSKADKVQQAGAFVAQLKRAMNRACKKT"
     gene            1335..2624
                     /locus_tag="VE170_14115"
     CDS             1335..2624
                     /locus_tag="VE170_14115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HYJ16627.1"
                     /translation="MASYSETLERIYKLHGGAIDLRLDRVAGALKLFDHPERKFPSIH
                     IAGTNGKGSTSAMLQRILSLSGYRAALYTSPHLVSFTERIKIGDEEISQDEVVAIADE
                     IWRRTAAVEITLTYFEVVTVMAFIYFARHPTDVAVIEVGLGGRFDATNVITPLVALIT
                     TISKDHEAFLGNDLLSIAGEKAGIIKPGVPVVCGAVNTAVGELFRQIANDRGSKAYFF
                     GEDFKFSLKNEGYFDYTGLKQHYFNLSLRLRGEHQRHNAAVAIAALETVQDRLPSSEA
                     AIRAGLATVQWPGRFEIMLERPTVVIDGAHNGEGVLSLVEELKVYRGRKIKLLFAVMS
                     DKDWRLMLARLIKVVSQVTFTKVAMERSADPAELARHFAAQVASRSHADACAALRGLI
                     DESAADDVIVVAGSLYLIGEIRPMLQEIAAARSRLPS"
     gene            2649..4970
                     /gene="lptD"
                     /locus_tag="VE170_14120"
     CDS             2649..4970
                     /gene="lptD"
                     /locus_tag="VE170_14120"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HYJ16628.1"
                     /translation="MTLRPLSLPFAALLALASLLANVWAAQGPQSAASNPAAPINLQA
                     DNLSSSDSGNQIEATGNVEVKREQTTLRASELRVNRATQDIEAKGQVTLDDPEWKIKS
                     ADSIRMNMERETGELHNADLFLEQGHISVSGRRFEKFGGQTYHIDDGFFTTCLCESGA
                     PSWKFSAEQMDLTLDGTGTIRNGYFYIMDVPVLYIPYGFFPLNSERQTGFLFPNIGSS
                     NKDGFQYLQPFFWAISKSTDATLSVDIETRTRFGVIGELRTQFDRNSDLKFESSYFNE
                     SWRQHRTVVDQTIANPNIPKDRWNIFDSHRYTTASDWLTYSDSAVYSDDLFTRELVGR
                     FDLPLGQEGNIRRSRYGESRMGLFKNWGDTFLKGEFNFFQDFIQDDKTTFMRTPQIGF
                     WGRRLLPGFPLELRWTVDGTNYIRRERGDGLRFDLRPEAVLPFKLASVVNGSLSVAPR
                     ETFYHLYSTPVNSSAHNVSRELIEMRGNLNTALSRIFAVDSLGLKQVKHVFEPEISYL
                     FIPGVNQNKIPIMDSIDRINRRNVVTFAVNNRLWGKSPSPLGDQILDSSTEYLSPTAT
                     GDVREFASFRMALSYDIDKERKGGDSLTDLDMRMRLTPASFLDLRLDGGVNPGAWQFT
                     QILAGVAITDPRPILRRSLDADFNRPNSVALSYHFLRRGPNSFLAENANADIDLCLIN
                     PNDSSCTKNTVGNINNSLLYHVTDNLLFNFSSTYDARDNRFIGFRAITKFMSFCECWS
                     VSFGVKHDVNPEKTSFSVNFGLLGLGNPKSAVR"
     gene            5294..5677
                     /locus_tag="VE170_14125"
     CDS             5294..5677
                     /locus_tag="VE170_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ16629.1"
                     /translation="MPTDRLTHPDKIVDKPWGYELIWAHTERYVGKVLHIKKGESLSY
                     QYHNVKDETIRLLSGMLELDIEKDGQQHKLRLAAGESLHIVPLMKHRMIAIEDCDVLE
                     VSTPELDDVVRLEDRYGRADSMARK"
     gene            5696..>7077
                     /locus_tag="VE170_14130"
     CDS             5696..>7077
                     /locus_tag="VE170_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYJ16630.1"
                     /translation="METQVTLAECLIERQMLSSEEMERVRQLQEEQQAPLSRLVVELG
                     FLSEEDLLPVMRDHFDLPLMSLRDVPNTPLPIDLPVNIGDFFKLARMVPVKIDGRDLI
                     VAIADPMELSRLHAIELATGLRVKAVLAKEKEIAARIEALYGGSYTADGTAEESAQGF
                     DGIAEDEDVAHLRDMASEVPVIRLVNQMLVRALESRASDVHIEPFENQLKVRYRIDGI
                     LHEVESPPRQLKGAVISRLKILAQLNIAERRLPQDGRIKTRLGGKDIDLRIATVPTLY
                     GESVVIRLLERGQIFTQLETMGFPPVQLARFNEMILKPHGMILVTGPTGSGKTTTLYG
                     ALQKINDPGKKIITIEDPVEYQLSGVNQIHVKPQIGLTFANGLRSIVRQDPDVIMVGE
                     IRDAETAEIAVQAALTGHLVFSTLHTNDAAGAISRLLEMGVQDYLLSSSLLGVLAQRL
                     VRRLCAHCRK"
BASE COUNT         1563 a         1894 c         1997 g         1623 t
ORIGIN      
        1 ttggactatc caggggtcgg cccagagttg agttatctgc gcgatcaagc ccgggtgcag
       61 tttgatgcgg caaccgacga ggaagcgatc gcgtcgatcc gtttactcgc tgaaatcgaa
      121 gggatcattc ctgccctgga aagcgcccat gcgatcgcgg tggtgcgccg cttggcgcca
      181 aaactcaaga aacgccaaat cgtcatcgtc aatctatccg gccgcggcga caaagatatg
      241 atcaccgtcg gcgaccatct cggagtgaag ctgtaagatt cgttcaaacg ttcaatagtt
      301 caaggttgcg gggtgggaga gggcgttgaa cgattgaact tttaaccgtt gaacggctag
      361 gttgatttta cttatgagcc gtatcgaaaa aaagtttatt gcagtgaagc gacgcggtga
      421 agcggcgctg gttccattta tcaccgccgg cgatcccgac ttggcgacta cgctgaaaat
      481 catgcgtgcc ttggaaacgg gcggtgccga tgccatcgaa ttgggcattc cgttttccga
      541 tccctcggcg gatggcccga cgattcagcg ttcgtcggag cgggctttga agaacccgat
      601 ctccttgccg gcgatattcc gcttggtacg cgatttccgc cgcacttccg aactgccaat
      661 tattctcttt ggttactaca atccgttttt ccgcttcggg ctcgacaagt tcgccacgca
      721 agcggcacaa gccggcgccg acggcgtact ctgcgtcgac ctgccgccgg aggagtgcgg
      781 cgagcttaaa agttgcaccg accgctgcga tctcgacatc atctttctct tgtcgcccac
      841 cagcggtgaa gaccgccggc gcttggtggc acgccatggg cggggcttta tctactatgt
      901 ctcggtgacc ggcgtcaccg gcgcgcgcca atctttcgcc accgatttgc gccgccaggt
      961 tgcgcaggtg cgtaaagtta cgtccttgcc agtgggcgtc ggtttcggca tttcgacgcc
     1021 caagcaagcg gccgcgatcg cccagttcgc cgacgccgta gtggtcggca gtgccctggt
     1081 gcagcgcatc gagcagggca aaagcaaagc tgacaaggtg caacaagccg gggcatttgt
     1141 cgcccagctc aaacgggcca tgaatcgagc gtgcaagaaa acctgatcgc gcttaaaaga
     1201 aagcgcggca actgctgatt gcgcagagca cctatccgga ttattcgcga gccacgatca
     1261 cggctgaacc ttatcgagtg acgacacttc ggcctgcggt ttcctggtag cgtagtaatt
     1321 ttaggctaag ccccatggcc agttattctg aaacccttga acgaatctat aaattgcacg
     1381 gcggggccat cgatttgcgc ctcgatagag tcgcgggcgc gctaaagctg tttgaccacc
     1441 cggaacgaaa attcccttcg attcatatcg ccggcaccaa cggcaaaggc tcaacctcgg
     1501 caatgctcca gcggatcctg tccctctcgg gttaccgtgc cgcattatat acatcgcccc
     1561 atttggtgtc gtttaccgaa cgcattaaga tcggtgacga ggagatatcg caagacgaag
     1621 tcgtggcgat cgccgacgag atctggcggc gcacggcggc ggtcgaaatc acattgacct
     1681 atttcgaagt ggtgacggtg atggctttta tctacttcgc ccgccacccc accgatgtgg
     1741 cggttatcga ggtcggcctg ggcggccgct ttgatgccac caatgtgatc acgccgttag
     1801 tcgcgctgat cacgacgatt tcgaaggacc atgaagcgtt tttgggaaac gatctactgt
     1861 cgattgccgg cgagaaagcc ggcatcatca agccgggtgt gccggttgta tgcggcgcgg
     1921 tcaatacagc cgtgggtgaa ctgtttcgcc agattgccaa tgaccggggt tcaaaggcct
     1981 atttctttgg tgaggatttc aaattttcct tgaaaaatga agggtatttc gattatacag
     2041 gattaaaaca acattatttc aatttatcgc taagactgcg tggcgagcat caaaggcata
     2101 atgccgcggt agccattgcg gcattagaga ctgttcagga tcgcctgccg agtagcgaag
     2161 cggcgattcg ggctggtttg gcgactgtcc aatggccggg acgattcgaa atcatgttgg
     2221 agcggccgac ggtggtcatc gacggtgccc acaatggtga gggcgtattg tcactggtgg
     2281 aggagctcaa agtttatcgc gggcgcaaga tcaagctgct gttcgccgtc atgtcggaca
     2341 aggattggcg gcttatgttg gcgcggttga tcaaggtagt ttcgcaagtg accttcacca
     2401 aagtcgccat ggagcgcagc gcggacccgg ccgagctggc gcgacacttt gccgcccagg
     2461 tggcgagtcg aagtcacgcc gatgcgtgcg cggcgttacg tgggctgatc gacgaatccg
     2521 ctgccgatga tgtgatcgtg gtcgcgggtt cgctttatct tatcggtgaa attcggccaa
     2581 tgttgcaaga aatcgccgcg gcgcgttcca gactcccaag ttgacaaggg ttccaggcac
     2641 gcaagatcat gaccttacga ccgctctcgt taccgtttgc cgccttactg gcattggcat
     2701 ccttgctcgc gaatgtctgg gccgcccagg ggccgcaatc ggcggcgagt aatcccgcgg
     2761 cgccgatcaa cttgcaggct gacaacttgt cgtcgagtga cagtggcaat cagatcgagg
     2821 cgaccggcaa tgtcgaggtc aaacgggaac aaaccacgtt gcgggcgagc gaattgcgcg
     2881 tgaaccgggc gacacaagat atcgaagcca agggtcaggt gacgctggac gaccccgaat
     2941 ggaaaatcaa atcagcggac tcgatccgca tgaacatgga acgggaaacc ggcgagcttc
     3001 acaacgccga ccttttcctc gagcagggcc atatcagcgt ctcggggcgc cgcttcgaga
     3061 agttcggcgg ccagacctat cacattgacg atggtttttt taccacctgt ctatgcgagt
     3121 cgggcgcgcc gtcgtggaaa ttttctgccg agcagatgga tttgactctt gatggaacgg
     3181 gcacgatcag aaatggctat ttctacatca tggatgtgcc ggtgctttac attccctatg
     3241 gattttttcc actgaatagt gagcgccaga ctggatttct atttcccaat attggcagct
     3301 cgaacaaaga cggttttcag tatctacagc catttttctg ggcgatctcg aaaagcactg
     3361 acgccacctt gtcggttgac attgagacgc gcacgcggtt tggagtcatc ggcgaacttc
     3421 gcacgcagtt cgatcgcaac tcggacctta agtttgaatc ttcctatttc aatgaaagtt
     3481 ggcggcaaca ccggacggtg gtcgaccaaa ccattgccaa tccaaatatt ccgaaggatc
     3541 gctggaacat tttcgactcc caccgttata ccactgcttc ggattggctc acctacagcg
     3601 acagcgcggt gtacagcgat gatttgttta cgcgtgaact ggtcgggcga ttcgacttgc
     3661 cgcttggcca ggagggcaac attcgccgca gccgctacgg cgagtcgcgc atgggtttgt
     3721 tcaaaaactg gggtgatacc ttcttaaaag gggagttcaa ctttttccaa gacttcattc
     3781 aagacgataa gaccacgttt atgcgcacgc cacagattgg cttttggggc cgacggttac
     3841 tcccggggtt tccactagag ctacgctgga cggtggatgg tacgaattat atccgccgcg
     3901 agcgcggtga cggtcttcga ttcgatctcc gccctgaagc ggtgctgccg ttcaaactcg
     3961 cttcggttgt aaacggttcg ttaagtgtcg cgcctcgcga aaccttctat catttgtaca
     4021 gtacgccggt gaattcctcg gcccacaatg tctcacgcga gctgatcgaa atgcgcggaa
     4081 accttaacac cgcgttgagc cgtatctttg cggtcgatag cttgggttta aagcaagtca
     4141 agcatgtttt tgaacctgag atcagttatt tatttattcc cggtgtcaat caaaataaaa
     4201 ttccgatcat ggacagcata gatcgtatta accgccgtaa cgtagttacc tttgcagtca
     4261 ataatcgcct atggggaaaa tcccccagtc ctctcggtga tcaaatcctt gacagcagca
     4321 ccgaatatct tagcccgacg gcgaccggcg atgtgcggga gttcgcatcg ttccgtatgg
     4381 ctttgtccta cgatatcgat aaagagcgca aaggcggcga tagccttacc gatcttgata
     4441 tgcgaatgcg tttgactccc gcgtcatttc tcgacttgcg tttggatggt ggcgtgaacc
     4501 ctggagcgtg gcagtttacc cagatcctcg ccggtgtggc gatcacagat ccgcgcccga
     4561 ttctgcggcg ctcgctcgac gccgatttca atcggccgaa ttccgttgcc ttaagttatc
     4621 acttcttgcg ccgcgggccg aacagttttc tcgccgaaaa cgccaatgcc gacatcgact
     4681 tgtgcttaat caatccgaac gactccagct gcacaaaaaa taccgtgggc aatatcaaca
     4741 acagtctttt ataccatgtt accgacaatc tgctgttcaa cttcagcagc acttacgatg
     4801 cccgcgataa ccggttcatc ggctttcggg cgatcactaa gttcatgtcg ttttgcgagt
     4861 gctggtcggt ttctttcggt gtgaagcacg atgttaaccc cgagaaaacc agcttcagtg
     4921 tcaactttgg tctcctcggc ttgggaaatc ccaagagtgc cgttaggtag gcgctttagt
     4981 aggtgacctg cggtgcaatc tgttcatcag tgaaatagct ggcgttcaga tgcaaatccc
     5041 tcgaatatag aatcaaacgt tgtcgctgtg cagcttgtgc cgacgcggta tctgaagctt
     5101 gcagatagca gcgccgtcat cggcctgccg agaataactc cggcagtgaa gtgcggtatg
     5161 gctttgccct ggggttatga acgttggtct tttggctcgc tctggaaatt gacgccggct
     5221 aaaccagctc tgctaacctc cttgcatata gtttagtttg ccgcaatggc ttaatctggt
     5281 atcattacca tcgatgccga ccgatcgatt gacccacccg gacaagattg tcgataaacc
     5341 atggggttat gaactgattt gggctcacac cgagcgctac gtcggcaagg tgttgcatat
     5401 caagaagggc gaatcgttga gttatcaata ccacaacgtc aaagatgaga cgattcggct
     5461 gctctccggc atgctcgaat tggatatcga aaaagatggc caacagcaca agctgcgttt
     5521 ggcggcgggc gagagtttac acattgtccc gttaatgaag catcgcatga tcgccatcga
     5581 agactgcgat gtcttagagg tctcgacgcc ggaactcgac gatgtcgtgc gcttggaaga
     5641 ccgttacggc agagcagatt cgatggcgcg aaaatagcgc aaaacaggaa tgagcatgga
     5701 aacacaagtg actctggcgg agtgtctgat tgaacggcag atgctctcca gcgaagaaat
     5761 ggagcgggtg cgccagctcc aggaggagca acaagcgccg cttagccggc tagtggttga
     5821 actcggtttt ttgtccgaag aagatctcct gccagtgatg cgcgatcatt tcgatctgcc
     5881 gctcatgtcg ctgcgcgatg tcccgaatac gccgttgccc atcgacctac cggtcaacat
     5941 cggcgatttt ttcaagctgg cccgcatggt gccggtcaag atcgatggtc gagacttgat
     6001 cgtcgccatt gccgatccca tggagctctc acgactacat gcaattgagt tggctacagg
     6061 tttacgggtc aaagcggtgc tcgccaagga aaaagaaatc gccgcccgga tcgaagcgtt
     6121 atacggcggc agttacacgg ccgatgggac tgccgaggag tccgcccaag ggtttgacgg
     6181 tatcgcggaa gatgaagatg tcgcccatct gcgcgatatg gcctcggaag tgccggtcat
     6241 tcggctggtc aatcagatgc tggtgcgcgc cctcgaaagc cgggcctcgg acgtgcatat
     6301 cgagcccttc gaaaatcaac taaaggtccg ctaccgcatc gacggcatct tgcacgaagt
     6361 cgagtcgccg ccgcgccaat tgaagggcgc ggtgatttcg cgtttaaaaa ttctcgccca
     6421 gctcaatatc gccgagcggc gcttgccgca ggacggccgg atcaagacca gattgggcgg
     6481 taaagatatc gatttgcgaa tcgccacggt gccgacgctc tacggcgaaa gcgtggtaat
     6541 ccgattgctc gagcgcgggc agatttttac ccagttggag acgatgggat ttccgccggt
     6601 gcagttagcg cgtttcaacg agatgatctt gaagccacat ggcatgatct tggtcaccgg
     6661 ccccaccggc agcggtaaga ccaccacgct ttacggcgcg ctgcagaaga tcaacgatcc
     6721 gggcaagaaa atcatcacca tcgaagatcc tgtcgaatat caattaagcg gcgtcaacca
     6781 gatccatgtc aagccgcaaa tcggtttgac ttttgccaac ggactgcgct cgatcgtgcg
     6841 ccaggaccct gatgtgatca tggtcggcga aattcgcgat gccgagaccg ccgagatcgc
     6901 tgtgcaggcg gcgcttacag gccacttggt gttttccacg ctgcatacca acgatgccgc
     6961 cggcgcgatt tcgcggcttc tcgagatggg tgtgcaggat tatctgctct cgtcgtcgct
     7021 actgggcgtg ctggcgcagc gcttggtgcg ccgcctctgt gcccattgcc gaaaaga
//
LOCUS       DASQKS010000552         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4916433, whole genome shotgun sequence.
ACCESSION   DASQKS010000552 DASQKS010000000
VERSION     DASQKS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4916433"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1177)
                     /locus_tag="VE170_14135"
     CDS             complement(167..1177)
                     /locus_tag="VE170_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16631.1"
                     /translation="MKRSRYTAAHFTFRLIIVTPIFSAQPVLAQTALEKMIVGYSAQA
                     GAYAPIWITKEAGLFRKHGLDVNLVFIPGGPTAAAAMIANEVQGMAMAGPAIVSSHLA
                     GSDLVMTAGIVNTFAFQLVTVKNITSANQLKGKRIGVNRFGAAPDVAARYALRHLKID
                     PREVTILQLGEQATRLEAMKAGQLDAAILLPPITTLAQKDGMNVLLDMSELGGEFLIT
                     GMASSQKYLTQNKPSASRFMRAFVEGIHYFKTQRRESEKIIARYMRTDNMEAVGATWE
                     YFAGKIVPRKPYPSVKGVKALLDLAAKERPEAAKAAPERFINATLLKELDDSGFIDGL
                     YR"
     gene            complement(1377..2354)
                     /locus_tag="VE170_14140"
     CDS             complement(1377..2354)
                     /locus_tag="VE170_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16632.1"
                     /translation="MAKQSLIWLLVVFFVANVSVAQAQQPTKIPRIGFLGGSASNTPR
                     IDAFRQGLRELGYVEGKNIVIELRFAEGILDRLPALAAELASLKVDVIVTGGPAATRA
                     AKEATSTIPIVMTNEVDPVGTGFVASLARPGGNITGLSTFAPELSGKRLELLKEVVPK
                     SSRVAVLGISTDPGYAQVLKELGLVAEALKVKLQYLDVLSPRDIETAFRAATKGRAEA
                     VLVLQSPVFNSQRTQVVELAVKNRLPTIYNHSEYVDDGGLMSYGVSFTDLFRRAATYM
                     DKILKGRTPADLPVEQPKKFEFIVNLKVAKQIGLTIAPNVLVRADRVIR"
     gene            complement(2539..3531)
                     /locus_tag="VE170_14145"
     CDS             complement(2539..3531)
                     /locus_tag="VE170_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16633.1"
                     /translation="MKPWIVFLICNIAAAIVNALAADGATLSKLRIGYPSPSAAFYPL
                     FATKEAGLLEKYGFDSEMVYVQGVKLVQVHVSGQLDVSTISSVVYLQASVGGADLIQV
                     ASSIDNQIMKLMVNPSIAKPQDLRGKTLAVTGFGSLTDLLLRPALKNWGLEPQKDVKI
                     VQIGRMPDIAIAIAQKTVDGGMVSFPTSVHAEKFNLKTLLDFAESGFDIPATTVVVSR
                     RYANANRDNLLRFLKAYIEGTKRLLTDRELGIRALRKYGGVTDRDMLATTHDLFASRY
                     IKKIPTINAKGVENSLSLIAENNPKAKGRKAEEFMDPSFMDELESSGFIKSVWP"
     gene            complement(3591..4424)
                     /locus_tag="VE170_14150"
     CDS             complement(3591..4424)
                     /locus_tag="VE170_14150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16634.1"
                     /translation="MKAFDVHVHPSTRGLDPHACGYFRRNLNDIPQTAEGFAALYAKN
                     EVRALLIGWHPSTVNEGARNSNEHVLDLANQFPETFPGILAALDTSAADLTIVARYAE
                     ELAASPKVKGFKFHPPDQGFYPNDKRFYDIWEIIQASGKPALFHVGFTVLGANTDGGS
                     GIALDHGRPIYLDTLARDFPKMKIIAAHPGWPWEQELIGVVTHKKNLFVDTSGYLAEQ
                     LPELFQKAIGGRLQDKALFGTDFPYVDLAKALASFDKMNFKPAVKDKLLFANAQHLFS
                     C"
     gene            complement(4444..>5404)
                     /locus_tag="VE170_14155"
     CDS             complement(4444..>5404)
                     /locus_tag="VE170_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYJ16635.1"
                     /translation="EIDNLRTLLVPADGVAALAQMHLVTEAVSEKAQIKLAVYKALRD
                     AGFAGLLTTNTSSVTRATLLRDGIVNRENFSLTHFFNPVLHTQMVEVVSGDMSATNRQ
                     AMLSFLKTLGRDPVPVQDISGFVSNGILMIYAVMAVRLLERGARIDQVDRAAKELKLL
                     PPFISFDSWKPSIVEDVTRIMAELRGDTFLRSSPLLSRLAKDNPRFYIDQKPNPQIYE
                     LANAHGQSLDDDNVKRALMTSMLVAAARIAELGEAAATVDFVATQGIKMPQPPLKQLD
                     MSGVTALREELEKTNRMLGASPLAIPTNLAETTGGYKTFYPRV"
BASE COUNT         1089 a         1477 c         1562 g         1276 t
ORIGIN      
        1 gacattattt tctcagctcc tctttaaaga tcgttcaagg ttcaagggtt caaggttcaa
       61 gggttcaagg ttcaatcgga atacggttac agtcgttcta aatgttccat tggttccatc
      121 cgagcgattg aacgcttgaa cttttgaacc cttgaacgag cttatttcat cgatacaagc
      181 cgtcgatgaa accgctgtcg tccaattctt ttagcagagt tgcattaata aagcgctccg
      241 gcgccgcttt ggcggcctcg ggacgttcct tggccgccag atcgaggagt gctttgacgc
      301 cttttaccga cgggtaaggt ttgcgcggca cgattttacc cgcgaagtat tcccaggtgg
      361 cgccgaccgc ttccatgttg tccgtgcgca tgtagcgggc gatgatcttt tcgctctctc
      421 ggcgctgggt cttgaaatag tgaatgcctt cgacaaaggc gcgcataaat cgacttgccg
      481 agggcttgtt ctgggtcaag tatttttgac tgctcgccat gccggtgatc aaaaattcgc
      541 cgcccaattc ggacatgtcg agtaaaacgt tcatgccgtc tttttgcgcc agggtggtga
      601 tcggcggcaa gagaatcgcc gcgtcgagtt ggcctgcttt catcgcttcc aaccgggttg
      661 cctgttcgcc caactgcaag atcgtcacct cgcgcgggtc gattttgaga tgcctgagcg
      721 catagcgcgc ggccacatcg ggcgcggcgc cgaagcggtt gacaccgatg cgtttgccct
      781 tgagctggtt cgctgacgtg atgtttttga ccgtgactaa ctggaatgca aaggtgttga
      841 cgattccggc cgtcatcacc agatccgaac cggcaagatg gctgctgacg atcgccggtc
      901 cggccatcgc catcccttgc acttcgttag cgatcatcgc cgccgccgcc gtcggtcccc
      961 cgggaatgaa aacgagattc acatcgagac cgtgctttct aaacagcccg gcttccttgg
     1021 tgatccagat cggcgcataa gcgccggcct gggcgctata gccgacgatc attttctcca
     1081 gcgctgtctg cgccagaacc ggctgagcgg agaagatcgg cgtcacgatg atcaacctga
     1141 aagtgaaatg agctgctgtg tagcgacttc gcttcatttt tagttccttc agtctgctca
     1201 cgcctcagtc agcttttcgc ttcaccgaat caccccatct gctggcattc cgattttggt
     1261 ctgcggggcc gcgtgcttga ctcatgcagg ggccgacctg cgtgtcggcc ttccgaacgg
     1321 acggacatta gagcgcggac attcttgtcc gcgcttaccc ttgcatgttg ccttcttcaa
     1381 cgtatcaccc tgtccgcccg taccaacaca ttcggcgcaa tcgtcaggcc gatctgcttg
     1441 gccactttca aattgacgat gaactcgaac ttcttcggct gctcgacggg gagatcggca
     1501 ggcgttctgc ctttcagaat tttgtccatg taggtagcgg cgcggcggaa caagtcggta
     1561 aagctcacgc catagctcat aagcccccca tcgtcgacgt attctgaatg gttgtatatc
     1621 gtcggaagcc ggttctttac cgcgagctcg acaacctgtg ttcgctgaga attaaacacg
     1681 gggctctgca gcacgaggac tgcctctgca cgccccttgg tggcggctcg gaatgcggtc
     1741 tcaatatccc tgggacttag tacgtctagg tattgaagct tcactttcaa cgcctctgcg
     1801 acgagtccca gctcttttaa cacttgcgcg tagcccgggt cggtcgaaat cccgaggacg
     1861 gccacgcggg aggacttagg aactacttct ttcagcagct ccagacgttt tccgcttagc
     1921 tccggggcaa aagtgcttaa acctgtaatg ttcccaccag gtcgcgccag gctggcgacg
     1981 aacccggttc caactggatc gacttcattg gtcatgacaa tgggaatcgt agaagttgct
     2041 tccttggcgg cacgggttgc tgccggacca cccgtgacga taacgtctac cttgagactc
     2101 gctagctcgg ccgcgagcgc ggggaggcga tcgagtattc cctccgcaaa tcgcaactca
     2161 ataactatgt ttttcccctc cacataccca agctcgcgca gaccctggcg gaaggcgtcg
     2221 atgcgaggcg tattcgaggc tgagccgcct agaaacccga tccgagggat tttggttggt
     2281 tgctgcgcct gggcgacgga aacgtttgca acaaagaaaa ctaccagtag ccagataaga
     2341 ctttgtttcg ccatgggtcc tgtgtgatta ctgatactga gaactggcta ccttaaacca
     2401 aatcaaaaac ctgtccagag taaagccaaa gggatcgtca tcccggcctt atcgtcacct
     2461 ggattcccgc ctgcgcggga attacggagc gtagtgcggt gttgtcttca ttttgactct
     2521 gcggatcgat gtttaagcct agggccatac cgacttgatg aatccgctcg attccagctc
     2581 atccatgaag cttgggtcca tgaattcttc ggccttgcgc cctttggctt tggggttgtt
     2641 ctcggcgatc aggctcaatg aattttccac gccttttgca ttgatcgtgg gaattttctt
     2701 aatgtagcgc gaggcgaaca gatcgtgagt ggtcgccagc atgtcgcggt cggtgacgcc
     2761 gccgtatttt ctgagcgcgc ggatgccgag ctcgcggtcg gttaaaagtc ttttggtgcc
     2821 ttcgatatag gctttgagaa agcgtagcaa gttgtcgcgg ttggcattgg cgtagcggcg
     2881 gctcacgacc acggttgtcg ccgggatgtc gaatcccgac tcggcgaagt cgagcagggt
     2941 tttcaagtta aacttttccg cgtggacgga agttggaaac gacaccatgc cgccgtcgac
     3001 ggtcttttgc gcgatcgcaa tcgcgatgtc gggcatgcgc ccgatctgca ctatcttaac
     3061 gtctttttgc ggctctaggc cccaattttt cagcgccggc cgcagcagca agtcggtgag
     3121 cgaaccgaat cccgttaccg cgagggtttt gcctctgaga tcctgcggtt tggcgatgct
     3181 cggattgacc atcagcttca tgatttgatt gtcgatggag ctggcgacct gaatcaaatc
     3241 ggcgccgcca acggacgctt gcagatagac gacggatgaa atcgttgaga catcgagctg
     3301 gccggagaca tgaacctgca ccagcttcac gccttgaacg taaaccatct cactgtcgaa
     3361 gccgtatttt tccaggagcc cggcttcttt ggtggcgaac agcggataaa acgccgcgct
     3421 cggcgaggga tagccgatgc gtagctttga aagcgtggcg ccgtcggcag ccaacgcatt
     3481 aacaatggcg gctgcgatat tgcagatgag aaaaactatc cacggtttca ttgcctaacc
     3541 tcccgcgcaa acggctccaa tgcagccgcg cagatccgcc ccgagtgcgg ctaacagcta
     3601 aaaaggtgct gtgcattggc gaacaagagt ttgtccttca ccgccggttt gaagttcatt
     3661 ttgtcaaagc tcgcgagggc ttttgccaaa tcgacgtagg gaaagtcggt gccgaacagc
     3721 gctttgtctt gcagccggcc gccgatggcc ttttgaaaca gttcaggaag ctgttccgcg
     3781 agatacccgg aggtgtcgac gaagagattc tttttgtgcg ttaccacgcc gattagctcc
     3841 tgctcccagg gccagccggg atgggccgcg ataattttca tcttggggaa gtcgcgggcg
     3901 agagtgtcca gataaatcgg ccggccatga tcgagggcaa tgccgctgcc gccgtcggta
     3961 ttggcgccca ggaccgtgaa gccgacatgg aacaatgccg gcttgccgct tgcctggata
     4021 atttcccaaa tgtcgtagaa ccgcttgtca ttgggataga agccttggtc gggcggatga
     4081 aatttgaaac ctttgacttt ggggcttgcc gccaactcct cggcgtagcg ggcgacgatg
     4141 gtcaaatccg ccgcgctggt atcgagggcg gcgaggatgc cgggaaaagt ttcgggaaac
     4201 tgatttgcca gatcgagcac atgttcgttg ctgttgcgcg cgccttcgtt gaccgtcgac
     4261 gggtgccagc cgatcaacaa cgctcgaact tcgttcttcg catagagcgc ggcgaagcct
     4321 tcagccgtct gcgggatgtc gttcaagttt ctgcgaaaat agccgcaggc gtgagggtcg
     4381 aggccgcggg tgctcggatg aacgtgaacg tcaaaggctt tcatggaagt tcgcttattg
     4441 agctcagacg cgtggataaa aagtcttgta gccgccggtc gtctccgcga gatttgttgg
     4501 gatagccaag gggctcgcac cgagcatgcg attggttttc tccagctcct cccgcagcgc
     4561 cgtaaccccg ctcatatcga gctgtttcaa tggtggttgt ggcattttga ttccttgtgt
     4621 ggcgacgaag tcgacggtgg cggccgcttc cccgagctcg gcgatccgtg cggcggcgac
     4681 taacatcgaa gtcataagtg ctcgtttgac gttgtcgtcg tcgagagatt gaccgtgggc
     4741 attggccaac tcatagatct ggggattggg cttttggtcg atgtagaatc tcgggttgtc
     4801 cttggccaag cggcttagca acggtgaaga gcgcaggaag gtatcgccgc gcaattccgc
     4861 cataatgcgg gtgacatctt cgacgatgga cggcttccag ctgtcgaagc tgatgaacgg
     4921 cggcaggagc ttcaactcct tggccgctcg gtcgacctga tcgatgcgcg cgccccgttc
     4981 gagcaagcgc accgccatga ccgcgtaaat catcaggatg ccgttggaca cgaagccgga
     5041 gatatcttgc accggcaccg ggtcgcggcc gagagttttc aggaaagata gcatggcttg
     5101 ccggttcgtt gcggacatgt cgccgctgac gacttcgacc atctgggtgt ggagcacagg
     5161 attgaaaaag tgcgtcagcg aaaaattctc gcggttgacg atcccgtcgc gcaaaagagt
     5221 cgcacgggtg accgacgatg tgttggtcgt cagcaggccg gcgaaaccgg cgtcgcggag
     5281 agctttatag accgcgagtt tgatctgagc cttttccgac accgcttcgg tgaccagatg
     5341 catctgcgcc agcgcggcta caccgtcggc aggcacgagc aatgtgcgaa gattatcgat
     5401 ctcg
//
LOCUS       DASQKS010000553         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1973787, whole genome shotgun sequence.
ACCESSION   DASQKS010000553 DASQKS010000000
VERSION     DASQKS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973787"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1164
                     /locus_tag="VE170_14160"
     CDS             100..1164
                     /locus_tag="VE170_14160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16636.1"
                     /translation="MQPKVKCSLFTEIQCPEGASPAQRLAEFMEQAELADRLGYDTYW
                     VAEIHCQPKFSLMSAPYVALGAAAQRTKRLRLGVAVNTLPVHHPVQLAEQSAMLDLVS
                     GGRMEFACGGGHPHSRAYECFGADHKATHEVMAEAMEVIRKAWSDEKLVHNGKFFQIP
                     EVIVNPKPVQKPLPPFWMATSSLDGVEVGARLGVNIFLPIHTRTPDQVYEFADAYWAG
                     LKKHSHDPKSRSLGLLVPMHIAKTTVEARVRAEAGVMSYFKTILDLRLDYTDWLNQRG
                     VELPARLRTAAGAMVGYDTVCEKHAVIGDAPFAVEKLKALCARTGATQVLGWFNIGTM
                     PDADVKDAMQRFAADVMPQL"
     gene            1339..1686
                     /locus_tag="VE170_14165"
     CDS             1339..1686
                     /locus_tag="VE170_14165"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYJ16637.1"
                     /translation="MEELTEQKCVACRVGAPAVTEDEIKELHPKISDWKIISEDSIPK
                     LDRLYKFKDFKDAIAFTDAVGAAAEDEGHHPRLTTEWGRVAVTWWTHKIKNLHHNDFI
                     MAAKTDAIYKKFV"
     gene            1709..>2724
                     /locus_tag="VE170_14170"
     CDS             1709..>2724
                     /locus_tag="VE170_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16638.1"
                     /translation="MGVRMLMKSLVSLACAWACPVAVSQAATHDDGFYRGKTIRLIVA
                     FSAGGGYDTYSRTIARHLGKYIPGNPNVVVDNMTGAGGIIHANYMFKQAKPDGLTIGN
                     NSGGLFLQQVMGAKGIEFDGQKFEYLGAPAVDHLVCAIAKASGITSMERWFAAKEPVR
                     FGGVGPGGFASDLPRVLQAALGLPIRVIDGYKGTSDIRLATESGELAGSCLSWDSYKT
                     TWRKQIESGEVNPIIQVMPKKHPELAAVASAMDYAKTDDAKKLIKHGAYDLAYSARPY
                     FLAPGTPKGRVEILRKAFADVLKDADLLAEAKKANFVIEYVTGEELAGIVLGLYKIEP
                     AFAA"
BASE COUNT          620 a          749 c          815 g          540 t
ORIGIN      
        1 gtcagcgcca ataacaagag aatcaagccg tgagcgaaag ccgcaccgac gcgatatcca
       61 gacccgaaaa cctccgtcgg tgacggtaaa atagagagga tgcagcctaa agtgaagtgt
      121 agtctattta ctgaaatcca atgtccggaa ggggcctcgc cggcgcagcg cttggccgag
      181 ttcatggagc aggcagagct cgccgatcgc ctggggtacg acacctattg ggtcgccgag
      241 atccactgcc agccgaaatt ttccttgatg tcggcgccct acgtcgccct cggcgcggcg
      301 gcgcagcgca ccaaacgctt gcgcctcggc gtcgcggtca acactctacc ggtgcatcat
      361 ccggtgcagc tggcggaaca gtcggcgatg ttggatcttg tcagcggcgg gcgcatggag
      421 tttgcctgcg gcggcggtca tccgcacagc cgggcctatg agtgtttcgg cgccgatcat
      481 aaagctaccc acgaggtcat ggccgaggcg atggaagtga tccgcaaggc ttggtccgac
      541 gaaaagctgg ttcataacgg caagtttttt caaattcccg aagtgatcgt caatcccaag
      601 ccggtgcaaa agccgctgcc gcctttttgg atggcgacca gctccctcga cggcgtcgaa
      661 gtcggtgcgc ggctgggcgt taatatattt ctgccgatcc atacgcgtac gcccgatcaa
      721 gtttacgagt ttgccgatgc ctattgggca ggattaaaaa agcacagcca tgacccaaag
      781 agccgctcgc tgggtttgct ggtgccgatg catatcgcca agaccaccgt ggaagctaga
      841 gtgcgcgccg aggccggcgt gatgagctat ttcaagacca ttctcgatct gcggctggat
      901 tacaccgact ggctcaatca acgcggggtc gaactgccgg cgcgcttgcg cacggcggcc
      961 ggcgcgatgg tcggctacga tacggtctgc gaaaaacatg cggtcatcgg cgatgcccca
     1021 ttcgccgtgg agaaactcaa agccttatgc gcccgcaccg gtgcgactca ggtcctcggc
     1081 tggttcaaca tcggcaccat gcccgacgcc gatgtcaaag acgccatgca gcgctttgcc
     1141 gccgacgtga tgccgcagct gtgatcggac cataacatgc accgcgaact acctagggca
     1201 gaggagccgc aaccaaaaga cgaatgaccg cactgaacgc agagcgcgca aaaaaggaag
     1261 gttacgacgt tcgtgatagg ataaaaaacc tttcgatcct gtatagtttg aagcgaattt
     1321 ctaaaaaagg agtttaatat ggaagagctg actgaacaga aatgcgtcgc gtgccgggtt
     1381 ggcgcgccgg cggtgaccga ggacgaaatc aaagagctgc accccaaaat ttccgactgg
     1441 aagattatca gcgaagacag cattcccaaa ctcgatcgcc tctacaaatt caaagacttc
     1501 aaagatgcca ttgcctttac cgatgccgtc ggcgccgccg ctgaagacga agggcaccat
     1561 ccgcgtctca cgacggagtg gggcagagtc gccgtcacct ggtggactca caagatcaaa
     1621 aacctgcatc ataacgactt catcatggcg gcgaagaccg atgcgatcta caaaaaattt
     1681 gtttagtccg agatggagtg ttggattaat gggtgttcgt atgttgatga aaagcttggt
     1741 gtcgttagct tgtgcctggg cttgtcccgt ggcagtcagt caggccgcaa cccatgacga
     1801 cggtttttat cggggcaaga cgatccgatt gatcgtggcg ttctctgccg gtggcggtta
     1861 cgacacttat tcgcggacga tcgcgcgcca tcttggcaaa tatattcccg gcaatcccaa
     1921 tgtcgtcgtc gataacatga ccggcgccgg tggcatcatt cacgccaact atatgttcaa
     1981 gcaggccaag ccggatgggc tgaccatcgg caacaactcg ggcgggttgt ttttgcagca
     2041 ggtgatgggc gccaagggga tcgaattcga cggccagaaa ttcgaatatc tcggcgcgcc
     2101 ggcagtcgat cacttggttt gcgcgattgc caaagccagc ggaattacgt ccatggagcg
     2161 ttggttcgcg gccaaggagc cggtgcgttt cggcggcgtc ggtcccggcg gattcgcgtc
     2221 tgatttgccg cgggtattgc aggcggcact gggcctgccg atccgcgtga tcgacggtta
     2281 caaaggcacc tcggacattc gcctggcaac cgaaagcggc gagctggccg gcagttgtct
     2341 gtcttgggat tcctacaaaa ccacctggcg caaacagatc gagtccggtg aggtcaatcc
     2401 gatcattcaa gtcatgccga agaaacatcc ggagctggcc gcggtcgctt cggcgatgga
     2461 ctacgcgaag accgacgacg caaagaagct gatcaaacat ggcgcctatg atctcgccta
     2521 ttcggcgcgg ccgtatttcc tggcgccggg aacgccaaaa gggcgggtgg agattttgcg
     2581 caaggcgttt gccgatgtgc tcaaggatgc cgacttgctc gctgaagcga agaaggcgaa
     2641 tttcgtcatt gagtatgtta ccggcgaaga attggcaggc atcgtgctcg ggctctacaa
     2701 gatcgagccc gccttcgcgg cgaa
//
LOCUS       DASQKS010000554         7091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1160478, whole genome shotgun sequence.
ACCESSION   DASQKS010000554 DASQKS010000000
VERSION     DASQKS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160478"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VE170_14175"
     CDS             complement(<1..237)
                     /locus_tag="VE170_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16639.1"
                     /translation="MKMHLFFALCLVQLIFFDWLPCAADGADSSTKVDYKVVPILQPM
                     KEVAPTPEINLTTPDGKKISLRDFRGKIVMLNFWA"
     gene            complement(1032..2129)
                     /locus_tag="VE170_14180"
     CDS             complement(1032..2129)
                     /locus_tag="VE170_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ16640.1"
                     /translation="MPNKILIVDDEPFNLDLLEQELMDYDYVLERATDGVDALAKADS
                     FSPDIILLDYMMPKMNGLEVVRALRQSERHKSVPVILLTAKATQEDKVAGLDAGADDY
                     VTKPFDSFELLARVRAMLRIKELHDQLGEWNRTLTEKVQQQVGELKRMERLKRYLSPQ
                     VAETILAREEDLFKTHRREITVVFLDLRGFTAFSDNAEPEEVMDLLRDYHAEMGKLIF
                     KFEGTLERFVGDGIVVIFNDPLPCEDHGSKAVNMAVEMRDRVKKLRVAWTKKGYDLDL
                     GVGLATGYATLGTIGFEGRMDYSSVGNLPNLAARMCAAAKGGQILTDQKTMSRLEEFF
                     DSEAFEEFQLKGLSRPVRVFNIVAAKAYRQS"
     gene            complement(2593..3639)
                     /locus_tag="VE170_14185"
     CDS             complement(2593..3639)
                     /locus_tag="VE170_14185"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16641.1"
                     /translation="MKNAGYARCFVPSDNPHSQNGRAEQNLRWKTLLAVVLTLLLCGA
                     RVHAQSKLPTIGWLSAVSGPPRGREEVIRRLRELGHVDGKTVAYEFRYADNKFERLPA
                     LADELVRLKVDLLIAPGTAGALALKQATKIIPIVFLDVTDPVAAGLVGSLAHPGGNVT
                     GFSSVESLLAGKRLEILKETVTKLSYAGALWDPRNPGSKLEWQDSQAAARELGLKLRS
                     LEVSNAEKYEDTLRDANKAGIGAVTVLSAPLAAAHQEKITRLAAKYRLPAIYVQENFV
                     TGGGLMSYGPNQNERYKRIAVFIDKILKGAKPADLPVEQPTKFEFIVNLKAAKQIGVM
                     IPPNVLVRADRVIR"
     gene            complement(3744..4109)
                     /locus_tag="VE170_14190"
     CDS             complement(3744..4109)
                     /locus_tag="VE170_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ16642.1"
                     /translation="MAKKKILIVDDTEFNRDLVIQLLEDDYDTVIAENGAEALTMTES
                     ERPDLILMDLGMPIMDGWEATRKLKANDALKHIPVIAVTSHAMVGDEIDARKAGCDDY
                     LPKPIDDELLIQKIKKFIG"
     gene            complement(4782..>7091)
                     /locus_tag="VE170_14195"
     CDS             complement(4782..>7091)
                     /locus_tag="VE170_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ16643.1"
                     /translation="GLITSGAIEIYYRYLESQETLALLQREVAAGAAFKIENFVQDIH
                     STLRGATRSREIAPKGLTNDFRFELEKLLLIAPSITEAIALNQQGGIQVQASRLRTVL
                     PDVKRDFATGAPFLQAKKGQSYFGPAYFVRGSEPYMTIAVPIERFAGDVIGVLQAEVN
                     LKYIGDVVTNLKIGKAGYSYVISRSGELIAHPDISLVLQRRNVLKLESTKAAFMPAAD
                     RPKRQALVTPNVQGKSVLTASAQVAGLEWAVIVDRPVEEAYEPLYASVLRTSTVLLFG
                     LGLALMASLFIGRRVVRPVGILREGVERIGAGDLSHRLTLKTGDEIEVLADEFNKMTA
                     QLQESYTNLEQKVDDRTMELTESLAQQTATSEILGVIASSPSDLQPVYQTILGNITRL
                     CEASIAALFLYDGETLALADSQGTTSEFADHLRSSRPRPSHATTTRLAALEHRMVHVT
                     DLLNDPAFAPSPRELYEKENVRTVISMPLIRENTLVGVITSWRREARPFTDKQIVLLK
                     TFADQAVIAIENVRLFRELGKKNDLLEAANKHKSQFLANVSHELRTPLNSIIGFTRIV
                     LRRTEGKIEQLQKDNLQKVLISSEHLLTLINELLDLAKIEAGRMDVYSEHFKLEEVLR
                     IATATVEPMLRNGNVKLVTEMPPDLPPLKSDRDKLKQIVLNLLSNSAKFTDRGEIKVA
                     AVRDDGMLRLTVSDTGIGMNPEALKYIFEEFRQVDMSTTRKYGGTGLGLAIVRRLAQL
                     LGGDIAVASQEGKGSQFTVTIPMELKIQP"
BASE COUNT         1446 a         1896 c         2028 g         1721 t
ORIGIN      
        1 ggcccaaaag ttcaacataa cgatcttgcc gcgaaagtcg cgcaaggaga tttttttacc
       61 gtcgggggta gtgagattga tttccggtgt cggcgcgacc tctttcatcg gctgcagaat
      121 cggcaccact ttatagtcga ctttggttga ggagtcggcg ccgtcggcgg cgcatggtag
      181 ccaatcgaaa aaaatcagct gcaccagaca aagcgcaaag aagagatgca tcttcattgt
      241 catcttactc tagccaactg tttaaagcgg cgcaatacat gctaatcgga cccgcgtcgc
      301 ggtaaattgg cttgtagatt taatccgggg taagactcgt ctctatactg gcgatatggc
      361 gttctgagac ggtcaagcac ttaagaaacg gagtttctaa aagcagaaag ccccccgaat
      421 gtttccggag ggcttctgtg atagataacc agaacggtta tctggtcgcc tgagagtaag
      481 gagggtttat aaaatcacca ccacaacccc acaactcaat tagctacttt ttctttttgg
      541 ctttcttttt ggcctttttc ttagctgcca taagcacctc cttgtaagat taaacgggcg
      601 ggtattctct tgataggaag aacagcttga ctcaggcgaa gacagtttac tatcagcaac
      661 cctcccatat tttgtatctg cgccgagtat agtgacacaa ttaatggtat gtcaagaaaa
      721 aaatactatt ctacaatttt ttttcaattt ttttattttg gcagcggtcg aaatcatgat
      781 ggcgtgatga agtttgcgcg caaccatgct ttatcaacaa acagtgcggt gttttatttc
      841 aaatgttcat ccctattgta gcggggtaaa gttgaaaaga gggattgccc acggcgcgtc
      901 ggctcactgc gtggatacag cgaacacaga tgagaagcga gatggctggc gctagcaaga
      961 tggacgacca gttcgcgcgc gcgagtatgt ttccgcaccg gtagaaaata cgacgcagga
     1021 ggaaataacc gttaggattg ccggtacgcc ttcgctgcga cgatattaaa tactcgtaca
     1081 ggacggctta ggcctttcaa ttggaactcc tcgaaggcct cactatcgaa gaactcttcc
     1141 aaccggctca tggttttttg atcggtcagg atctgcccgc ccttggccgc ggcgcacatg
     1201 cgcgccgcga gattaggtag attaccgacg gaactatagt ccatgcgtcc ttcgaaaccg
     1261 atggtgccca gggtcgcgta gccggtggct aagccgacgc cgaggtcgag atcgtagccc
     1321 ttcttggtcc acgcgacacg cagcttcttg acccggtcgc gcatctcgac cgccatgttg
     1381 acggctttgc tgccatggtc ctcgcacggc agcggatcat tgaagatcac cacgatgccg
     1441 tcaccgacga agcgttccag tgtgccttcg aatttgaaaa tcaacttgcc catctctgcg
     1501 tgataatcgc ggaggaggtc catgacttcc tcgggctccg cattatcgga gaacgccgtg
     1561 aagccgcgca agtcgaggaa cacgacggtg atttcgcggc gatgggtttt gaacaggtct
     1621 tcttcccttg ccaggatggt ctcggcgacc tgcggcgaga gatagcgctt caaacgctcc
     1681 atgcgcttca gctccccgac ctgttgctga accttctcag tcagagtgcg gttccactcg
     1741 ccgagctgat cgtgcagctc tttgatgcgc agcatggcgc gcacccgcgc cagtaactca
     1801 aaggaatcga acggcttggt gacataatcg tccgcgccgg cgtcgagccc ggcgaccttg
     1861 tcttcctggg tcgccttggc cgtgaggaga atcaccggca cgctcttgtg gcgctcactc
     1921 tggcgtaacg ccctgacgac ttcgagccca ttcatcttcg gcatcatata gtcgagcagg
     1981 atgatatccg gactgaaact gtcagccttg gcgagtgcgt caaccccgtc ggtggcgcgc
     2041 tcgagcacgt agtcgtagtc catcagctcc tgttcgagga gatcgagatt aaagggttcg
     2101 tcatcgacga ttaagatttt gtttggcata ggtcacccat taccacttta gcctgcgcgg
     2161 ctgcttttgg attgtccgac aaactcgccg agcgttgttg ggctgatgtt tattcagctg
     2221 atcacgaata tttcgcaata ccgcctcgtt actcgttagc ttaagcattc tctgggctgt
     2281 tttgattcta cagtcgcaaa attctgtggc gagcccggcc ataagttcgg atttgttgtc
     2341 accgcggcgg tattctgcgt tgtatattgt tttgcgagct agccaaattc ggagcgactc
     2401 gctcaaggtc ctcgatacga tccttctgcg cccgcgtggg gagtcagtga taggatagcg
     2461 acgtggggaa tcgttttcgc cggttggcgt gcctaggatg ggcgatagcg agaaggatct
     2521 cgtcattagc aaacataaga tcgctttcct catgaatttg tcaccgacgg tttcgttgat
     2581 aacaggcgac tgttaacgga tgacccggtc cgctcttact agtacattgg gcgggatcat
     2641 cacgccgatc tgcttcgctg ccttcagatt gacgatgaat tcaaatttgg tcggttgctc
     2701 gacggggagg tcggcgggtt tggcgccttt tagaatcttg tcgataaaaa cggcgatgcg
     2761 cttgtaacgt tcgttttgat tggggccgta ggacatcaac ccgccgccgg taacaaaatt
     2821 ttcttgaaca tagatcgccg gcaggcggta tttcgcggct aatcgcgtga tcttttcttg
     2881 atgggcagcg gctagcggtg ccgacaacac ggtgaccgcg ccgatgccag ctttgttggc
     2941 gtcgcgcaag gtgtcttcgt atttttccgc attgctgact tccagggagc gaagtttcaa
     3001 gccgagctcg cgcgccgccg cttgactgtc ttgccattcc agtttcgagc ctggattgcg
     3061 cggatcccac agcgcgcctg cgtaagaaag tttagtgacg gtctctttca ggatctccag
     3121 ccgtttgccg gctaacagcg actctacgct gctgaagccc gtgacattgc cgccgggatg
     3181 agccaggctg ccaacgaggc cggcggcgac cggatcggtt acgtctaaga aaactatggg
     3241 gattatcttg gtcgcttgct tcaaggcgag ggcgccggca gtgcccggcg cgatcagtaa
     3301 atcgaccttg agacgaacca gctcatcggc cagtgccggc agccgctcaa acttgttatc
     3361 agcatagcga aactcgtaag cgacggtctt gccgtcgaca tggccgagct cacgcagccg
     3421 ccgaataact tcctcgcgac cgcgcggagg accggacacc gcggagagcc aaccgatcgt
     3481 cgggagcttc gattgcgcgt gcacgcgggc gccgcatagt agaagggtga gaacgacggc
     3541 gagtaaggtt ttccatctta gattttgctc cgcgcggccg ttctgggagt gtgggttgtc
     3601 ggacggtacg aagcaccggg catacccagc gtttttcaaa ttgacacctt ttggattgtc
     3661 tgcatcacgc ggcagcatcg cttgtcgagg cggcctcacc atgtttcggt atcccaatcc
     3721 aacatcgaaa atccaaaatg gcatcagccg atgaatttct taattttctg tatcagcagt
     3781 tcgtcgtcaa tcggcttggg cagataatcg tcgcagccgg ctttacgcgc gtcgatctcg
     3841 tcgccaacca tggcgtggga ggtgacggcg atgacgggga tatgcttcag cgcgtcattt
     3901 gctttcaact ttcgcgtcgc ttcccatccg tccatgatcg gcatgccaag atccatcaaa
     3961 attagatccg gccgctcgct ctcagtcatg gtcaacgctt ctgcgccgtt ctccgcgatc
     4021 accgtgtcat agtcgtcttc gagtagctga atgaccaagt cgcggttaaa ttcggtgtcg
     4081 tcgacgatga gtatcttttt ctttgccatg acaattcctt tcggaagttt cgtaaggggt
     4141 gaatggtaag gggaaggcga tttcgaacgc tgcccgatct gttaattctg atgcatttgc
     4201 ttctcttgag gtggcgtatc agtccggtga tcgttttgtc gattcgatcc attcgcgctc
     4261 tgagcatttg ctcgcttaag ctgaatctct cgcggtttag aaatttgtca gtctcgcgag
     4321 agacatcaat cgctaaatct ttcgatggct tccaagcatc gagtcttttg tgcgcttttc
     4381 accacccaac atgacgaagg ggtctccagc ttcgagccgg aggatagccg tcgccgcgaa
     4441 gtatccgccg attcagtctc gccagaggcg agcgttcgct cgactcgttg ggtactggca
     4501 ctggaacgta ttggagatca gtgaagtttt cagcgcacag caactcgtcg gcgatccact
     4561 ccgggctccg gcaggtgttc ggaatctgcg gaatcctgat tcgtttagtc gccagcggcg
     4621 actcggcggc gaacgtattg gagtgaagtc cgagaagagc cgtacttctc gcatcagcaa
     4681 ggttggttaa aaattctctg cgattccaat gattagattc ttggttattc atcccgctct
     4741 ccgatcatta aactcacgcc gcaccacttg ccgttgaccg tttaaggttg gattttcagc
     4801 tccatcggta tcgtcaccgt gaactgagag cctttgcctt cttggctggc gaccgcgatg
     4861 tcgccgccga ggagttgggc gagacgtctg acgatggcaa gacccaagcc ggtgccgccg
     4921 tattttctcg tcgtcgacat gtcgacctgg cgaaactcct cgaagatgta tttcaaggct
     4981 tccgggttca tgccaatgcc ggtgtcagaa accgtgagtc tgagcatgcc gtcgtcgcgt
     5041 acggcggcga ctttgatctc ccccctgtcc gtgaacttgg cagagttgct gagcaggttg
     5101 aggacgatct gcttcaactt gtcgcggtcc gatttaagcg gcggcaggtc gggcggcatt
     5161 tcggtgacca gcttgacgtt accgtttctg agcatgggct cgacggtggc ggtggcgatg
     5221 cgcagaactt cttcgagctt gaagtgctcc gagtacacgt ccatgcgtcc ggcttcgatc
     5281 ttggcgagat cgagcagctc gttaattagg gtcaagaggt gctccgaact gatcaggact
     5341 ttttgcaaat tgtctttttg cagttgctcg atctttccct cggtgcggcg cagcacgatg
     5401 cgcgtgaaac cgatgatcga gttcaagggt gtgcgcagct catggctgac gttggccaag
     5461 aattgggatt tatgtttgtt ggcggcttcg agcagatcgt ttttcttgcc cagctcgcgg
     5521 aacaggcgga cattctcaat cgcgatcacc gcctgatcgg cgaaggtttt gagcagcaca
     5581 atctgtttgt cggtgaatgg gcgggcctcg cgccgccagg aagtgatcac gcccaccaga
     5641 gtgttttcac ggattagcgg catggagatg acggtgcgga cattttcttt ttcgtaaagc
     5701 tcgcgggggc tgggcgcgaa agccgggtcg ttgagcagat cggtgacgtg caccatgcga
     5761 tgctccagag ctgccaaccg ggtggtcgtg gcgtggctcg gtcgcggccg gctgctgcgc
     5821 aagtgatcgg caaactcgct ggtggtgccc tggctgtcgg cgagggcgag agtttcgcca
     5881 tcatagagaa acaacgccgc gatactcgct tcgcacaagc gagtgatatt tccgagaatg
     5941 gtttggtaga ccggctgcaa atcgctcggc gaactggcga tgacgcccaa aatctcgctg
     6001 gtggcggttt gctgtgccag cgattcggtc aattccatgg tgcggtcgtc gaccttttgc
     6061 tccaagttgg tgtacgattc ctggagctgc gcggtcattt tattgaactc gtcggccaga
     6121 acctcgatct catcgccggt ttttagcgtc aaccgatgac tcaaatcacc ggcgccgatg
     6181 cgctcgacgc cctcgcgcag gatgcccacc ggccgaacca cccggcgacc gatgaataga
     6241 ctcgccatga gcgccaggcc gaggccaaac aagagcacgg tggaggtgcg taagaccgac
     6301 gcatagagcg gttcgtaggc ttcctcgacc gggcggtcga cgatgaccgc ccactcgagc
     6361 ccagcgacct gcgccgatgc ggtcaagaca cttttacctt ggacgttggg ggtcaccagc
     6421 gcttggcgct tgggccggtc ggcggccggc atgaaagcgg ccttggtcga ttctaatttc
     6481 aacacgttgc gccgctgcaa gaccaaactg atatcgggat gggcgatcag ctcgccgctg
     6541 cggctgatga cgtaggagta gccggctttg ccgattttta gattcgtcac cacgtcaccg
     6601 atgtatttga gattgacctc agcctggagc acgccgatga cgtcgccggc gaatcgctcg
     6661 atcggcacgg cgatggtcat gtagggttcg gagccgcgca cgaagtaggc cggaccgaag
     6721 taagattgcc cctttttggc ttggaggaac ggcgccccgg tggcgaagtc gcgcttgacg
     6781 tcgggcagca cggtgcgcag acgcgacgct tggacttgga tgccgccttg ttgattgagc
     6841 gcgatcgcct cggtgatcga cggcgcgatc aacaacagct tttccaactc gaaacgaaaa
     6901 tcgttggtca gccctttggg cgcgatctcc cgactgcgcg tcgcgccacg cagcgtgcta
     6961 tgtatgtcct gaacgaagtt ttcgatcttg aacgccgcac cggcggcgac ctcgcgctgc
     7021 aacagcgcca aggtctcttg gctctcgaga tagcgatagt aaatctcgat ggctccgctg
     7081 gtgatcaggc c
//
LOCUS       DASQKS010000555         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1162372, whole genome shotgun sequence.
ACCESSION   DASQKS010000555 DASQKS010000000
VERSION     DASQKS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162372"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VE170_14200"
     CDS             <1..471
                     /locus_tag="VE170_14200"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ16644.1"
                     /translation="SHLAFAGMKILEIGCGIGLSSLVLQRHGADITASDYHPLAEEFL
                     RFNSDLNGLPPIKFHNAAWAGPNPLLGRFDLIVGSDLLYERGHPAQLSVFLACHTEYA
                     ARVILADPGRSRCGQFSSQMSSAGYRRTEQKFHCAGAAGAKPRGRMMSFARGQI"
     gene            497..802
                     /locus_tag="VE170_14205"
     CDS             497..802
                     /locus_tag="VE170_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16645.1"
                     /translation="MFFGARSLLSHQASTTLVGMTQRSTEEKSRYKISDSHRNQPFVG
                     VMKRDRDTYSWTWKGHIDFADGHYFEFASQRSFDTASEAEAYMRRYACDRINTRMNA"
     gene            979..1659
                     /locus_tag="VE170_14210"
     CDS             979..1659
                     /locus_tag="VE170_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16646.1"
                     /translation="MSKWSDVEKQLNVMVNQWYDTAEIKRLLAVPLNPARLAFRHQHM
                     NFFSNNRRDCWAAVACKAPLDVKRAIWEHEKDELIFDPRMGKAHVTEEDLGDRKESDM
                     IPGARAAFYAWLHCAQEKPWLEGLACSHILERRNNDQIVTGGTLTQRLVKRQEVELGI
                     KKKDQDINVQVHLVADVDHTDLLSDVFQRYVVDGLTAQEILRGAEESLAIERSFHGAV
                     AAAMAEME"
     gene            1770..2912
                     /locus_tag="VE170_14215"
     CDS             1770..2912
                     /locus_tag="VE170_14215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16647.1"
                     /translation="MDIRHGLISADSHVVTEPKAFLDRMSKNRFGARIPQIKEVETKG
                     QTVERWFVDDKPLHSRGVCNCPAVMGDPQRNLYPQRWQEVPAKAYLPADRLQALDADG
                     IDAEVLFPNDPSSFHQYNDAEFEIACVQAYNDSLGELTHASQRFISLAMIPLLCGIET
                     IVAEVRRAESKGHRGIVMFALPSLMVASLPHIGEPYWYPLWEVCQELAMPIHFHASAG
                     LARKLTFPEWDGYTPYQQHAAFTVPTASWPAQIVPNLIFSGIAYKFPQSKWVFAETGI
                     GSVSCVQSACDHEWERRRLWRHGLARRPSELIREQVYVNFWYEQAGIELRHAVGVDNI
                     MWESDYPHIASTYPDSWQFVERSLAGVSPEERKKMLYGNAQALYRL"
BASE COUNT          662 a          922 c          828 g          612 t
ORIGIN      
        1 agccatttgg cattcgccgg gatgaagatt ctcgagattg gttgcggcat cggcttgtcg
       61 agcctagtct tacagcgcca tggcgcagat atcaccgctt ccgattatca tcccctggcg
      121 gaagaatttc tgcgtttcaa cagcgatctg aacggcctgc cgccgatcaa gtttcacaat
      181 gctgcctggg ccgggcccaa tcccctgctc ggccgtttcg acctgatcgt cggcagcgac
      241 ttgctctacg aacgcggtca tcccgcccaa ttgtcggtgt ttttagcttg tcacaccgag
      301 tatgccgcac gagtcatcct cgccgatccc ggccgcagta gatgcggaca gttcagcagc
      361 caaatgagct cggcaggcta ccggcgcacc gaacagaagt ttcactgcgc cggtgcggca
      421 ggcgccaaac cgcgcggccg catgatgagc tttgcgcgcg gccaaatata aattggccaa
      481 gccccacggt taagtcatgt tcttcggcgc cagatcattg ctatcgcacc aggcaagcac
      541 tacactggtc ggcatgaccc agagatctac tgaagaaaaa tcccgctaca agatttccga
      601 ttcgcaccgc aatcagccat tcgtcggggt catgaaacgc gatcgcgata cttactcgtg
      661 gacttggaaa ggtcacatcg attttgccga cggacattat ttcgaatttg ccagccaacg
      721 cagttttgac accgccagcg aggccgaagc ttacatgcgc cgctatgcct gcgaccgcat
      781 caatacccgc atgaacgcat gaccgcatcg cgtctccgga cataagtgca tttcacttcc
      841 cgcattagct cccaattcca tcgattctcc caaccaacgc tagcccggtt ggtttgacag
      901 ccgtttcggc cccgacatat attcgcgtca gacccgcgag gcccgcgccc gcgcaatctt
      961 gttttgaagg aacatgcgat gagcaaatgg tccgacgtcg aaaagcaatt gaatgtcatg
     1021 gtcaaccagt ggtacgacac agccgagatc aagcgcctgc tcgccgtgcc gctcaatccc
     1081 gccaggctcg cctttcgcca ccagcacatg aacttcttca gcaacaaccg gcgcgactgt
     1141 tgggccgcgg tggcctgcaa ggcgccactc gacgtcaaac gcgccatctg ggagcacgaa
     1201 aaagatgagc tcatctttga cccgcgcatg ggcaaagccc atgtcaccga ggaggatctg
     1261 ggcgaccgga aagagtctga catgatcccc ggcgcgcgcg cggcatttta tgcctggctg
     1321 cactgtgcgc aggagaaacc ttggcttgaa gggctcgcct gctcgcatat cttggagcgc
     1381 cgcaataacg atcagatcgt caccggcggc acgctgacgc aacggctggt gaagcgccag
     1441 gaagtcgagc ttggcatcaa gaagaaagat caagacatca acgtccaagt gcatctggtc
     1501 gccgacgtcg atcataccga tctcctatct gatgtttttc agcgctacgt tgtcgacggt
     1561 ctgactgcgc aggagatcct gcgcggcgcc gaagagtccc tcgccatcga gcgcagcttt
     1621 cacggcgccg tcgccgcagc aatggcggag atggaatgaa agtaaatttt cactcgccgc
     1681 tcgctcggtt cgagcgtagg aaccgcagct cattcgtcca cctgccccgc atgccggagt
     1741 atctacttta acgctaacgg aacatcgcag tggacattcg tcacggtctg attagcgccg
     1801 actcccatgt cgtcaccgag cccaaggcgt tcctcgatcg catgtcgaaa aaccgatttg
     1861 gcgcgcgcat tccgcaaatc aaagaggtcg agaccaaggg ccaaacggta gaacgttggt
     1921 tcgtcgacga caaaccgtta cacagccgcg gcgtgtgcaa ctgcccggcg gtgatgggcg
     1981 atccacagcg taatctttat ccgcagcgct ggcaggaagt gccggccaaa gcctatctgc
     2041 cagcggaccg cttgcaagcc ctggatgccg acggcatcga tgccgaagtg ctatttccca
     2101 acgacccaag ctcttttcat caatacaacg acgccgagtt cgagatcgct tgcgtgcaag
     2161 cctacaacga ttctttgggc gaacttaccc acgcgagcca gcgctttatt tctctagcta
     2221 tgattccttt gctctgcggc atcgagacca tcgtcgccga ggtaaggcgc gcggaaagca
     2281 aaggtcaccg cggcatcgtc atgttcgctc tgcccagttt gatggtcgca tcgctgcctc
     2341 atatcggcga gccgtactgg tatccgctgt gggaggtttg ccaagagcta gccatgccga
     2401 tccacttcca cgcgtccgcg ggattggcgc gcaagctaac tttccccgaa tgggacggct
     2461 acacacccta ccaacaacat gcagccttta ccgtgccgac cgcgtcatgg ccggcgcaga
     2521 tcgtgcccaa cttgattttc tccggtatcg cctacaagtt tccgcaatct aaatgggtgt
     2581 tcgccgaaac cggcatcggc tcggtgagct gcgtgcagtc agcctgcgat cacgagtggg
     2641 aacgccgccg gctatggcgt catggtttgg cgcggcgccc gagcgagctg attcgtgaac
     2701 aagtgtatgt gaacttctgg tacgagcagg ccggcatcga gctgcgtcat gccgtcggcg
     2761 tcgacaacat catgtgggaa tccgactacc cgcatatcgc gtcgacctat cccgactcgt
     2821 ggcagttcgt cgaacgctcc cttgccggcg tatcgccgga agaacggaag aaaatgttgt
     2881 acggcaacgc ccaggcattg tatcggcttt agagaagtat gaatggccgc acagaacgca
     2941 aagggacgca aagtaaaatc ccaagacgaa ttccggctgt aggggcgaat catgattcgc
     3001 cctaggggtg cggagcggcg accg
//
LOCUS       DASQKS010000556         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_277295, whole genome shotgun sequence.
ACCESSION   DASQKS010000556 DASQKS010000000
VERSION     DASQKS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277295"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..788)
                     /locus_tag="VE170_14220"
     CDS             complement(21..788)
                     /locus_tag="VE170_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16648.1"
                     /translation="MELNGRVALVTGGAGGIGGAVVRALAKSGVAGVVVNYRKSSKEA
                     EALAAEIGQDGVKALAVQADVQSDSQVRAMMQTIQDRFGRLDMVVNNAGVTHWVKLPD
                     LEALTDPIWDEILDVNVKGAFRCARAAQALLEASHGMIVNVSSISGVLAPSTMSSLAY
                     GTAKAALIYMTKGLAVAMAPKVRVNCVAPAFTDTPWMSQHFGVDYQQVIAKASTGYPL
                     QRIATPEDVAGAILGLITGGDFVTGQTLLVDGGLSLS"
     gene            complement(878..2602)
                     /locus_tag="VE170_14225"
     CDS             complement(878..2602)
                     /locus_tag="VE170_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYJ16649.1"
                     /translation="MGNKVIDPVTLEVIWNRFLSVANEQQDALIRTAFSTIVRESQDL
                     ACGMFDTKGRMISQSISGTPGHINAMATSMKHFLAAFPPDKLAPGDVLITNDPWQTAG
                     QINDITITTPIFKSGKLVALFANTCHSADIGGRILSAEAREVFEEGLRIPIMKLFDRG
                     EPNRILMQIVRTNVRQPDEVIGDFYAQTSCNETGGRALREMMDEFNLDSIDEVSEEII
                     RRSEAAVRAEIAKLPAGEWSSETWSDGFEEPILIRCTVRIAGEEIYIDFTGSSPQSSR
                     GINVVLNYTHAYASFAIKAAICPDVPHNEGSFRPVHVSAPEGSILNALDPAPVASRQV
                     IGHFIPSAIFAAMSGALPGRLMAPGADPIWLSVWRGHDPSFTLTVFQVGGTGARPSKD
                     GLNAVGFPSGVAGVPAEVIESLSPVVLKRRELRADSGGPGTWRGGLGQLTEFARRGDG
                     KWSVSSIADRTQYPALGLLGGKAGAAGEVSTGDGVKLHAKALKDLAKGEVIHVNLPGG
                     GGYGDPFQRAPEKVLWDVIEGYVTAEAAERQYGVAVVYRGAADALVKLPEEWQVDMAR
                     TAALRRGH"
     gene            complement(2592..>2851)
                     /locus_tag="VE170_14230"
     CDS             complement(2592..>2851)
                     /locus_tag="VE170_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYJ16650.1"
                     /translation="DMKLKLPRNSTQGGDPRKGSRMAFFAETGGYVETAIYDRYALAP
                     GMLFAGPAIVEERESTLIVGANGRAHVDERLNVIVELGDGK"
BASE COUNT          557 a          929 c          804 g          561 t
ORIGIN      
        1 catactgctt gactcgattt tcaactgagg ctcaacccgc catccactaa cagcgtttgc
       61 ccagtcacga agtcgccgcc ggtaatcaat ccgagaattg cgccggcgac gtcctcgggt
      121 gtcgcgatgc gttgcagcgg ataacccgtg gacgctttgg caatcacctg ctggtaatcg
      181 acgccaaagt gctggctcat ccacggcgta tcggtaaatg ccggcgcgac gcaattgacg
      241 cgcaccttgg gcgccattgc cacggccaag cccttggtca tgtaaatcaa agccgccttc
      301 gccgtgccgt aggccagcga cgacatcgtc gacggcgcca gcacgccgga gatcgatgac
      361 acattgacga tcatgccgtg gctggcttca agcagcgctt gcgcggcgcg ggcgcagcga
      421 aacgcgccct tgacgttgac gtcgagaatc tcatcccaga tcggatcggt gagagcttcg
      481 agatccggca gtttcaccca gtgcgtcaca ccggcgttgt tgacaaccat atcgagccgg
      541 ccgaaacgat cttgaatagt ctgcatcatc gcccgcacct gggaatcgct ttgcacatcg
      601 gcttgcacgg ccagcgcttt gacgccgtcc tgcccgatct ccgccgccaa tgcttcggct
      661 tccttactcg acttccggta gttcacgacg acccccgcga caccagactt cgccagcgcg
      721 cgcaccaccg cgccgccgat gccgccggca cccccggtca ccaaagctac tctaccgttc
      781 agctccatat gttcgcttcg ctctcaattg caaattacaa attccatatt ccagatttct
      841 ccgatttgta atatggaatt tggaatctgc aattccttta atgccctcgc cgcagcgccg
      901 ccgtccgcgc catatcgacc tgccattcct ccggcaactt gaccaacgca tcggccgcac
      961 cgcggtaaac gacagccacg ccgtattgcc tctcggcagc ttccgccgtc acgtaacctt
     1021 cgatgacatc ccagagaact ttttccggcg cgcgctggaa cgggtcgccg taaccgccgc
     1081 cgcccggtag attaacgtgg ataacttcgc ccttggctaa atccttgagt gctttggcgt
     1141 ggagcttaac gccgtcgccg gtgctcactt cgccggcggc gcccgctttg ccgccgagta
     1201 atcccaaggc gggatattgg gtgcgatcgg cgatggagct aacactccac ttgccgtcac
     1261 cgcgccgagc aaattccgtg agctggccca agccgccgcg ccaagtgcct ggaccacccg
     1321 agtcggcgcg cagttcgcgc cgttttaata ccaccggcga caggctttca atcacctcag
     1381 ccggcacacc ggcgacgcca ctgggaaagc cgacggcgtt gagaccgtct ttcgacggac
     1441 gggcgccggt gccgccaact tgaaatacgg tcaacgtaaa cgacgggtcg tggccgcgcc
     1501 agacgctcag ccaaatcgga tcggcccccg gcgccatcaa ccgtccgggc aatgcgcccg
     1561 acatggccgc gaagatcgcg ctgggaatga aatggccgat cacctggcgg ctcgccaccg
     1621 gcgccggatc gagagcattg agaatgctgc cctccggcgc gctgacgtgc accgggcgaa
     1681 aactgccttc gttatggggc acgtcgggac agatcgccgc cttgatcgcg aagctcgcgt
     1741 aagcatgagt atagttcaac acgacattga taccgcggct gctttgcggc gacgagccgg
     1801 tgaagtcgat gtagatttct tcgccggcga tgcgcaccgt gcagcggatc aggatcggct
     1861 cttcaaaacc atcgctccac gtctcgctcg accattcgcc ggccggcagc ttggcgatct
     1921 cggcgcgcac cgccgcttcg gagcggcgga tgatttcctc ggaaacctcg tcaatggagt
     1981 cgaggttgaa ctcgtccatc atttcgcgca gcgcccggcc gccggtttcg ttgcaagaag
     2041 tttgagcgta aaaatcgccg atcacttcgt caggctggcg cacgttggtg cggacgatct
     2101 gcatgagaat cctattgggc tcgccgcgat cgaacaattt catgatcggg atgcgcaacc
     2161 cctcttcgaa aacctcgcgc gcctcggcgg agagaatccg cccgccgata tcggcgctat
     2221 ggcaggtgtt ggcgaacagc gcgaccagct tgccgctctt gaaaatcggc gtggtaatcg
     2281 tgatgtcgtt gatctgcccg gcggtctgcc agggatcgtt ggtgatcagg acatccccgg
     2341 gcgccagctt atccggcgga aacgcggcga ggaaatgttt catcgacgtc gccatggcat
     2401 tgatatggcc gggcgtgccg ctgatcgact gcgagatcat ccgacccttg gtgtcgaaca
     2461 tgccgcaagc taagtcctgg ctttcgcgca cgatggtgct gaaggcggtg cgaatgagcg
     2521 cgtcctgctg ctcgttggca accgacagga aacggttcca aatcacttca agcgtaactg
     2581 gatcgatgac tttatttccc atcgccaagc tccacaatga cgttcagccg ctcgtccacg
     2641 tgcgcccggc cgttggcacc aacgatcaga gtcgactcgc gctcttcgac aatcgccgga
     2701 ccggcgaaca gcatgcccgg tgccaaggca tagcgatcgt aaatcgcggt ctcgacataa
     2761 ccgcccgtct cggcaaaaaa cgccatacgc gagcctttgc gcgggtcacc gccctgagtt
     2821 gagttacgcg gcagttttag cttcatgtcc g
//
LOCUS       DASQKS010000557         6235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6200815, whole genome shotgun sequence.
ACCESSION   DASQKS010000557 DASQKS010000000
VERSION     DASQKS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6200815"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VE170_14235"
     CDS             complement(<1..464)
                     /locus_tag="VE170_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ16651.1"
                     /translation="MKIVEQLPEYIRTLIPYEPGKPIEEVEREYGIANSTKLASNENP
                     LGPSPKALAAIRAKLDELHLYPDGDCFYLKNGLAKKLGVAPDELIFGNGSNEIIELAV
                     RAFMRPGDQAVMARQAFVVYQLIVQAVGGRSVQVALREFTHDLSAIADAVNA"
     gene            complement(461..1537)
                     /gene="pheA"
                     /locus_tag="VE170_14240"
     CDS             complement(461..1537)
                     /gene="pheA"
                     /locus_tag="VE170_14240"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HYJ16652.1"
                     /translation="MSGKESVDVLRKKIDQIDKKIVNLLNSRAALAQRIGHSKGLSSQ
                     EVYVPNREKEILQRVSASTRGPLPAGAIRSVFREIISGCRSLEAPLRVAYFGAEATYS
                     HLAAKEKFGSSAELCSTATIAEVFQEVSQDRAALGVVPIENSTEGAVAHTLDCMVDSD
                     LQICAEVYLDIHHHLLSKSGKPADIRRIMSHPQALAQCRGWLTAHFPKITVEEVASTA
                     HGAIAAANDGGIAAISSALAKEVYNLVAVASNIEDRSNNITRFLVIGKMLTKPCGEDK
                     TSLVFSAKDKPGALFQMLQPFAKSRINLSKIESRPMKSKPWEYLFFLDLMGHREQAPV
                     KRAIAELEKNCVFLKVMGSYASGR"
     gene            1682..2566
                     /locus_tag="VE170_14245"
     CDS             1682..2566
                     /locus_tag="VE170_14245"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYJ16653.1"
                     /translation="MREIVLGTNESGKKLENYLKKEFPIGYVRKLFRKHGIRLNGARA
                     KPEDAISAGDKIQIYIPFDAQPTRPATTFAVPKLDVVFEDGDLLIIDKPAGLAVHEGK
                     TVSKRDSVIGILERQYRASQSNPQLVHRLDQDTSGLLMVAKNLKTAEELKLAFETGAV
                     DKEYLALVAGRLANDQGKIDTPLPGRDGNPVRALTRYKVVKRYSEVTLVRVAIETGRM
                     HQIRLHFAQLGHPVVMDDHHGDFGLNKKFRREFGLKRQFLHAAKLHLNLAGKVCEWEA
                     KLPNDLDRTLALLATQKP"
     gene            complement(2609..3244)
                     /locus_tag="VE170_14250"
     CDS             complement(2609..3244)
                     /locus_tag="VE170_14250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ16654.1"
                     /translation="MLDVRPIGIEDYAERCSKPLSELHDKLWVETYSITRNPQMMIGA
                     LEGQFLKMIVGLTGARRILEIGMFTGYSTLAMAEALPKGGTIVACEIDRVAVDIAKRY
                     FAESAHNDKIEVRVGAAVDSLKRIWGPFDLCLIDADKENYGIYYDACLELTRPRGLIV
                     LDNVLRDGRVLDPQDDCTKSLHALNKRIRSDPRVENVLLPMRDGIMLVRKL"
     gene            complement(3296..4045)
                     /locus_tag="VE170_14255"
     CDS             complement(3296..4045)
                     /locus_tag="VE170_14255"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16655.1"
                     /translation="MKLKGKVAFVTGFGSGLGQAIAVMFAKEGAAVAGISKTEAKGLE
                     TVAAIERAGGKALFRAGDICDSAQMKALIDHTVKQFGGLDILVNSAGVRTNGSITEIT
                     EDDWDRTLDTNLRAVFVASRLAIPEMKKRGGGVILHIAARSGMLGQAGRAAYCASKGG
                     MVTLTEAMAMDHAQDKIRVNCICPGPTRTPMVDTSTPEKLARYKTRVPIGRIGEPEDI
                     AYAAVYLASDEASMVTAAILPVDGGMRLTGS"
     gene            4150..5406
                     /locus_tag="VE170_14260"
     CDS             4150..5406
                     /locus_tag="VE170_14260"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYJ16656.1"
                     /translation="MTDSNDPRIPVTILTGFLGAGKTTLLNRILTADHGRRVAVIVNE
                     FGEVGIDHHLLISSDQEVVEMSNGCICCSVRGDLLRSLFQLLEHREKFDTLMIETTGL
                     ADPAPVVQSFFIDERIKSEYALNGVVTVVDAKHILQQLSSSPEAKEQIAFADMVLLNK
                     IDLINPDDLPELEFKLRNLNGAARVCQTRNSDIDIATILDLRALDLEIKATKHDHNHA
                     HTEDIETVAIETPGDLDGVKISQWFRELIAEFGERIMRMKGILNLRKDPDQFVFQGVH
                     LLFEGRPGRPWAETEERMNRLVFIGRNLNKEKITQGFMNCITTDGEYASSDDVDPYGK
                     KQEVSKFTLDQIRYWVQTILEFPTGIPIVVKEVPCVKAGCPPVETALMVFLKNEPPRT
                     FKILARINDVNFDHVYNLIENPLPCC"
     gene            complement(5537..6232)
                     /locus_tag="VE170_14265"
     CDS             complement(5537..6232)
                     /locus_tag="VE170_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16657.1"
                     /translation="MGAAYCRSLAAEGAAVVIADILDGEPVAAEIRTNGGKALALRVD
                     VSNDSDCQRMAAEAIKAFGRIDILVNNAAIFINIQRHPFYEITGDEWDKVSAVNIKGP
                     FLCAKAVFPQMKEQKSGKIINISSSTAYWGTPNFLHYVASKAALIGMTRSLAREVGEF
                     GICVNAIAPGLVEHEGQNAPKALTELQLKERSIKRLQTPDDLMGTLVFLCSSDSDFMT
                     GQAIVVDGGSVFH"
BASE COUNT         1404 a         1843 c         1661 g         1327 t
ORIGIN      
        1 tcggcgttga cggcatcggc gatggcgctg agatcatggg tgaattcgcg cagggccact
       61 tgcacgctgc ggccgccgac agcttgaacg atcaattgat ataccacgaa cgcctggcgc
      121 gccatcaccg cctgatcccc cggtcgcata aaggcgcgca ctgccagttc gataatctca
      181 ttggagccgt tgccaaagat caactcgtcg ggagccacgc caagcttttt cgccaagccg
      241 ttttttagat aaaaacagtc gccgtcagga tagagatgaa gctcgtcaag cttggcgcga
      301 atcgcggcca gcgctttggg cgacggcccc aaaggattct cattcgacgc gagcttggtg
      361 gagttggcga tgccatactc ccgttccacc tcttcgatcg gcttacccgg ctcgtaggga
      421 atcaaagtgc gaatatattc gggtagttgc tcgacgatct tcatctacca ctcgcgtagg
      481 agcccatgac ctttagaaag acacaatttt tttccagttc ggcgatcgct ctcttcaccg
      541 gcgcttgttc tcgatgcccc ataagatcca aaaaaaacag gtactcccaa ggtttgctct
      601 tcatcggccg ggactcgatc ttggacaaat tgatccggct cttggcgaag ggttggagca
      661 tttgaaacag cgcgccaggc ttgtctttgg cggagaatac caacgaggtt ttgtcttccc
      721 cgcagggctt ggtcagcatc ttaccgataa ccaggaaacg tgtgatgttg ttgctgcggt
      781 cttcgatatt actggccacc gccaccaggt tgtaaacctc tttggccaaa gcgctcgaga
      841 tggcggcaat gccgccgtcg ttggcggccg cgatcgctcc atgggcggtg ctcgccacct
      901 cttccacggt aattttcggg aaatgggccg tgagccagcc gcggcactgc gccagcgcct
      961 ggggatggga catgatccgc cggatatctg ccggcttgcc gctcttggac aaaagatggt
     1021 ggtgaatatc caaatacact tcggcgcaaa tttgcaggtc cgaatcgacc atacaatcga
     1081 gcgtgtgcgc caccgcacct tcagtcgaat tctcgatcgg taccaccccc aatgcggcac
     1141 ggtcctggct gacctcttga aagacctccg cgatggtcgc ggtcgaacac agctcggccg
     1201 aagaaccgaa tttttctttc gccgccagat ggctgtaggt cgcttcggct ccgaagtagg
     1261 cgactcggag cggcgcttcg agcgaccggc agcccgaaat aatttcacga aataccgaac
     1321 ggatggcgcc agccggaagc gggccgcgag tcgatgcgga gacccgttgt agaatctcct
     1381 tctcgcgatt aggaacgtaa acctcttgac tggataggcc cttgctgtgt ccgatacgtt
     1441 gtgccagcgc ggcccgggag tttagcaggt taacgatttt tttatcaatc tgatcgattt
     1501 tttttcttag aacatcgacc gactcttttc cactcaatga cgacctcacc gggcgcaaac
     1561 caacaaggag agtctccctg gcgccgtgcg acgtaaccta tcttaataat tagagaattg
     1621 caaatattac cgctccccaa gcttgactca gcggaagttc tcccgtttac tcggcgatgc
     1681 catgcgggaa attgtcctgg ggactaacga gagcggcaag aaacttgaga actacctcaa
     1741 gaaagaattc ccaatcggct atgtgcgcaa gctatttcgc aagcacggca ttcgcttgaa
     1801 cggcgcgcgc gcaaaaccgg aagatgccat cagtgccggc gataagatcc aaatctacat
     1861 tcccttcgac gcgcaaccga cacgtccagc gacgaccttt gcggtaccca agctcgacgt
     1921 cgttttcgag gacggagact tgctgatcat cgacaaaccg gctggcttgg ccgtgcacga
     1981 aggcaagacg gtcagtaagc gcgactcggt tatcggcata ttagaacggc aataccgcgc
     2041 gagtcagtcc aacccgcagc tcgttcatcg cctcgatcag gatacttccg gccttctgat
     2101 ggtagcgaag aatctaaaaa ccgccgagga gttgaagctc gccttcgaaa ccggcgcggt
     2161 cgataaagag tatctcgcct tggtcgcggg ccggctagca aatgatcagg gcaagatcga
     2221 cacaccgttg ccgggacgcg atggaaaccc ggtgcgcgct ctgacccgct ataaggtagt
     2281 caaacgttac tccgaagtca ctcttgtccg ggtcgccatt gaaaccggcc gcatgcatca
     2341 aatccgcttg cacttcgctc aactcggcca tcccgtggtt atggacgatc accatggcga
     2401 ctttggattg aacaaaaaat ttcgcaggga attcggcttg aagcggcaat ttttgcacgc
     2461 ggcaaagctg caccttaact tagccggcaa agtatgcgag tgggaagcga aactgccgaa
     2521 cgatctcgat cgaactctcg ctttactggc gacccaaaaa ccttgaaggg cggggcgtag
     2581 gctcggccca acgcgcggtc aaagcccctt acaacttgcg caccagcatg atgccatcgc
     2641 gcatcggtag caggacattc tcgacccgcg gatcgctgcg aattcttttg ttgagggcgt
     2701 gtaaagattt ggtgcagtcg tcttgcgggt cgagcacccg tccgtcccgc aggacgttat
     2761 cgaggacgat caagccgcgt ggccgggtca attccaggca tgcgtcatag taaatgccgt
     2821 agttttcctt gtcggcgtcg atcaaacaaa gatcgaaagg accccatatc cgtttgagac
     2881 tatccaccgc cgcgccgacc cgcacttcga tcttgtcgtt gtgcgcgctt tcggcaaaat
     2941 agcgtttggc gatgtccaca gccacccggt cgatctcaca cgccacaatg gtgccgccct
     3001 tgggcagcgc ctcggccatt gccaaagtgc tgtagccggt gaacatgccg atctctaaga
     3061 tccgtcgcgc gccggtgagg ccaacgatca tcttgaggaa ctgcccttcc agagcgccaa
     3121 tcatcatctg cggattgcgc gttatggagt aagtctcgac ccacagctta tcgtgcagtt
     3181 ccgataacgg cttgctgcac cgctccgcgt agtcttcgat ccctatcggt ctaacatcga
     3241 gcattcaact gtccttgaca agcattctaa atcgctgatc actcatcgcc cactctcacg
     3301 accccgtcaa ccgcatgcca ccgtccaccg gcaagatcgc cgccgtcacc atagaagcct
     3361 cgtcggacgc caaataaacc gccgcatagg cgatgtcttc gggttcaccg atacggccga
     3421 tcggcactct ggttttgtag cgcgcgagtt tttccggcgt cgaggtgtcg accatcggcg
     3481 tgcgtgtcgg gccgggacaa atgcaattca cgcgaatctt gtcctgggca tgatccatcg
     3541 ccatcgcttc ggtgagcgtc accatgccgc ccttggaagc gcaataagcg gcccggccgg
     3601 cttgaccaag catgccggag cgcgccgcga tatgcaggat caccccgccg ccgcgttttt
     3661 tcatctcggg gatcgccaga cgggaggcga cgaaaacggc tctaagattg gtatcgaggg
     3721 tacgatccca atcatcttcg gtgatctcgg tgatactgcc attggtgcgc acgccggcgc
     3781 tgttgaccag gatatcgagc ccgccgaact gcttcaccgt gtggtcgatg agagctttca
     3841 tttgcgcgga gtcgcagata tcgccggcgc gaaacagcgc cttgccgccg gccctttcga
     3901 tcgccgcaac ggtttccaaa cctttcgcct cggtcttgga aatgccggcc actgccgcgc
     3961 cttccttggc gaacattacc gcgatggctt gacccaagcc ggagccgaaa ccggtaacga
     4021 aagcgacttt accctttagt ttcatcgcca aaccctccgt aggaagtcaa gatatacaat
     4081 tgctgcaagg ttgactcaac tatcggccgt tgttattttt tccgagtccg ctacgaggaa
     4141 atttcactta tgaccgacag caacgatcca cgcataccgg taaccatcct aaccgggttt
     4201 ttgggcgccg gcaaaaccac gctcctgaat cgcatcctga ccgcggacca tggccgccgc
     4261 gtcgccgtca tcgtcaatga gttcggcgag gtcggcatcg atcaccatct attgatctcg
     4321 tcggaccagg aagtggtcga gatgagcaac ggctgcatct gctgcagcgt tcgcggcgat
     4381 ttattgcgca gcctgtttca actgctcgaa caccgggaga agttcgacac cttaatgatc
     4441 gaaaccacgg gcttggccga tccggcgccg gtggtccaaa gctttttcat cgacgaacgg
     4501 atcaagagcg aatacgctct gaacggcgtc gtcaccgtgg tcgacgccaa acatattttg
     4561 cagcagctga gcagttcacc cgaagccaag gaacaaatcg ccttcgccga catggtgctg
     4621 ctcaacaaga tcgatctgat caatccggat gatctgccgg agttggaatt caagctgcga
     4681 aatctcaacg gcgccgcgcg tgtctgccag acgagaaatt ccgacatcga catcgccacg
     4741 atcctcgacc tgcgcgccct cgatctggaa atcaaagcca ccaagcatga ccacaaccat
     4801 gcacacaccg aagatatcga gaccgttgcc atcgaaaccc ccggcgatct cgacggcgtc
     4861 aagatcagcc aatggttccg cgaactgatc gccgaattcg gcgaacggat catgcgtatg
     4921 aagggaatac taaatctgcg caaagacccc gatcaattcg tcttccaggg tgtccacttg
     4981 ctgttcgaag gccgcccggg acggccctgg gcggaaaccg aagagcggat gaatcgatta
     5041 gtcttcattg gccggaattt gaacaaggaa aaaatcactc aaggcttcat gaattgcatt
     5101 accacggacg gcgaatacgc ctcgtcggac gacgtcgacc cctacggcaa aaagcaagaa
     5161 gtttctaaat tcaccctcga tcaaatacgt tattgggtgc aaaccattct ggaatttccg
     5221 accggcatac cgatcgtcgt caaagaagtc ccctgcgtca aagccggctg cccgccagtg
     5281 gaaaccgccc tgatggtttt cctgaaaaac gaaccgccgc gcaccttcaa aatcctcgcg
     5341 cgcatcaacg acgtgaattt cgatcatgtc tacaacttga tcgagaatcc gctgccatgc
     5401 tgctgaagta ataaagaagt ggggattttc tcgcccgctc cggaacccag tcaccaagtt
     5461 ttcgataact tggagcaaaa gctagctctt ggggcgtaaa tcctgaagcg tacttccgac
     5521 gaccgatgag ctcgagttag tgaaaaacac tgcccccgtc cacgacaatc gcctgcccgg
     5581 tcatgaagtc gctgtcggac gagcagagaa aaaccagcgt gcccatgaga tcatccgggg
     5641 tttgcagccg tttgatggac cgctctttga gttgcagttc ggtcaacgcc ttgggcgcgt
     5701 tctgaccttc atgctcgacc aaacccggag cgatggcatt gacgcaaatg ccgaactcgc
     5761 cgacttcgcg cgctaacgac cgggtcatgc cgatcagcgc cgctttcgat gcaacatagt
     5821 gcaaaaagtt cggcgtgccc cagtacgcgg tcgacgacga gatgttgatg atcttgccgc
     5881 tcttctgctc tttcatctgc ggaaagaccg ccttggcgca gagaaacggc cccttgatat
     5941 tgaccgccga cactttgtcc cactcgtcgc cggtaatttc gtaaaacgga tggcgctgga
     6001 tgttgatgaa gatggcggcg ttgttgacca gaatatcgat gcggccgaaa gcttttatgg
     6061 cctcggcggc catgcgctgg caatcgctgt cattcgaaac atcgactctg agcgccagtg
     6121 ccttgccgcc gttggtgcga atctcggccg ccacgggctc gccgtcgagg atatcggcaa
     6181 taacaaccgc cgcgccttcg gcggccagac ttcgacagta cgccgcgccg atatg
//
LOCUS       DASQKS010000558         5764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4920332, whole genome shotgun sequence.
ACCESSION   DASQKS010000558 DASQKS010000000
VERSION     DASQKS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4920332"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VE170_14270"
     CDS             complement(<1..579)
                     /locus_tag="VE170_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ16658.1"
                     /translation="MLLINNKVVEQILDMKGCIEALDTGYRDLVNERANYRGRYDFFV
                     PNDDPKLMYRWGTMEGASRSFETFAIRMKSDMLEWPDGKTVEKYCVQPGTFCGFVMVF
                     STKNGEPLAIINDGVIQHMRVGACAGLGVKYLSREDSKTVGILGSGGMARTYLLAFNE
                     VRKINKVKVYSPTKKNRDAYAEEMGNRLGIETI"
     gene            complement(752..1198)
                     /locus_tag="VE170_14275"
     CDS             complement(752..1198)
                     /locus_tag="VE170_14275"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HYJ16659.1"
                     /translation="MVIYNLLCKKKHSFEGWFPSFEDFQKQADKKLISCPTCGTTKVE
                     KVPHACAVHVKKDQPPAAPAKTAVPAPAPQPNAAEFKEMLLRVHHYVKENFEDVGPRF
                     AEEAKQIHKGEAAERPIHGTATLSEVKELAEEGVPCMPLPKPELDS"
     gene            1357..2517
                     /locus_tag="VE170_14280"
     CDS             1357..2517
                     /locus_tag="VE170_14280"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ16660.1"
                     /translation="MSEQISLRVVQKIADVPRERWDALVGGATPFLKWDWLESLEQSG
                     CVNDKSGWLPHHIIVEKDGGLIAACAMYLKLHSMGEFVFDYEWAEAAQHAGIRYYPKM
                     LVGVPFTPVTGHRFITAPGEDRPHLIQFMGQALAKIAVDNKISSVHVNFCLADEREAL
                     EAVGFLPRHGIQYHWQNRSFHSFDDYLASFRSDRRNKVKRERREVAERGITIRAYEGA
                     ELTQKQLRTMFRLYKGHIDRLYYGRQYLTQEFFDELHKRFAAHLCLMLAERDGKIIAG
                     TFNVRDDQAMYGRYWGCVEEHPFLHFNVCYYSAIEHSIRSGLERFEAGAGGSFKQLRG
                     LEPEHTTSVHYIVDGQFRSAVERFLTQERELIRRKRDMQLEHSQLKREQEEP"
     gene            complement(2616..3950)
                     /gene="mgtE"
                     /locus_tag="VE170_14285"
     CDS             complement(2616..3950)
                     /gene="mgtE"
                     /locus_tag="VE170_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYJ16661.1"
                     /translation="MPVRVHHHGTINFTPAELYDAWPVLSRAEKIEGFEFLQRDDAES
                     FFLQLNGRDRADLMLGLTPAERRLWMRLLALDDAADLIQEAPAQDRAALLAMLDDPTQ
                     REVKGLLDYAEDDAGGLMNPRYSRLRPDMTVGEAISYLRRDAQSRARTTYYAYVVDHD
                     ERLLGVVTFRDLIVAPADKSIGEVMRSEIISARDDMDQEALSKLFMRHHLLMIPVVDG
                     AGRIKGIVNVDDIVDVVQEEATEDIQKIGGVESLDGPYLQVSLPKMIRKRAGWLAALF
                     LGEMLTATAMGYFEAEIARAVILALFVPLIISSGGNSGSQATTLVIRAMALGEVRPRD
                     WWRVIRREFATGLALGSILAAIGLVRILLWQFLFDAYGEHYFLIALTVGLSLVGVVMW
                     GSLMGSVLPFVLRSLGFDPASASAPFVATLVDVTGLVIYFTAAAIVLRGTLL"
     gene            4079..4855
                     /locus_tag="VE170_14290"
     CDS             4079..4855
                     /locus_tag="VE170_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ16662.1"
                     /translation="MKSSWKNVAEPVVFFLVLLVLWEILVELIKVPSFILPPPRDLWT
                     AFFKKLPILGHHSLITFIEAFGGFALSLVLGVGFAISVVYSRHLQNTIYPLIVILYAM
                     PKSAFAPLMVIWVGYGMFSKISIAFLVAFFPIVVNTVVGLKEVEPELLELARINRGSA
                     WDVFKKIRLPNSLPYMFAGIKVAIVLSVTGAIVAEFVAANEGLGYLVLQANYSLDTAF
                     ALVILLILAVLSLGLFWLAELCQRKLAPWSAAVREVEGSF"
     gene            4879..>5764
                     /locus_tag="VE170_14295"
     CDS             4879..>5764
                     /locus_tag="VE170_14295"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16663.1"
                     /translation="MKLLLSTLMALAIALFDSGAALEAAELTPANVRMDFIIGGKHAP
                     WYVALEKGFYAKRGLAATIQASTGSADTVRTIGSGGADFGFADAATMIVAKSRGTPVQ
                     AAAQFGYVGATILWRDEGVIKSIKDLPGKSLAISPGQAQWFLMPAYARINKIDFKAIK
                     IQETAAPIQPAALATKKADFIIMFRASNDEVAEMAASKVGVKLSRVFMKDTGLDIYGT
                     SLIVKDEDIKKRPEFVRAYVEGTLEGLRYARDNQEEALKILLKHKPELDSVLTTQQLK
                     NALTEVFLPLESVNVGMGF"
BASE COUNT         1218 a         1550 c         1717 g         1279 t
ORIGIN      
        1 aatagtctct atgcccaatc tattacccat ctcttcggcg taagcgtcac gattcttctt
       61 ggtcgggctg tagactttga ctttgttgat cttgcgcacc tcgttgaacg ccaggagata
      121 ggtgcgcgcc atgccgccgg aaccgagaat gccgacggtt ttggaatctt cgcgggataa
      181 gtacttcacg ccgagaccgg cgcaggcacc gacgcgcata tgttggatga cgccgtcgtt
      241 gatgatcgcc agcggctcgc cgttcttggt cgagaatacc atgacaaaac cgcagaacgt
      301 gccgggctgg acgcagtact tttccacggt tttgccatcg ggccattcaa gcatgtcgga
      361 cttcattctg atcgcgaagg tctcgaatga acgcgaagcg ccttccatgg tgccccagcg
      421 gtacatcagc ttgggatcgt cgttggggac gaaaaaatcg tagcggccgc gatagttggc
      481 gcgttcgttg accaggtcgc ggtagccggt atcgagggct tcgatgcagc ccttcatgtc
      541 gaggatttgt tcgacgactt tgttattgat cagtaacatg gcgcctcctg tttcagctat
      601 ttcgtagaag cgcggcgaat gcaagggagg atgttaccga tacctgccga cgaatggcac
      661 tcggaaaggc cggacggctc gagagtagtg tgaaatgaaa aacgccgctt ttgcggcgct
      721 tgctaagaac tgttttgccg gaggcgaggt attaactatc gagctcgggt ttgggcagtg
      781 gcatgcaggg cacgccttct tcggcgagct ctttgacttc gctgagggtg gcggtgccgt
      841 gaatcggtcg ctcggccgct tcgcccttgt gtatctgctt ggcttcctcg gcgaagcgcg
      901 gcccgacgtc ttcgaaattt tctttgacgt agtgatggac gcgcaggagc atttctttga
      961 attcagcggc gttaggctgt ggcgcgggag ccggtacggc tgttttcgcc ggcgccgcgg
     1021 gcggctggtc tttctttacg tgcacggcgc aggcgtgcgg cactttttcg actttggtcg
     1081 tgccgcaggt gggacaggag ataagctttt tgtcggcctg tttctgaaaa tcttcgaagc
     1141 ttggaaacca gccttcaaaa ctgtgtttct tcttgcagag gagattgtaa atgaccataa
     1201 gaaaccaccg cagagatcat caataaggtc accaggacag cttgtcaagg gtaggtgcat
     1261 ggcacaatgg gaatcgaccg gccaaaaagc gcttgcgatt ccaatccttg gcgcaaaagt
     1321 ataaaagagc cacagtttca gctaatatta caaccaatga gcgagcaaat tagcctgcgg
     1381 gtggtgcaga aaatcgccga tgtgccgcgt gagcgttggg atgcgctggt cggtggcgca
     1441 acgccgttcc tcaagtggga ttggttggaa agtctcgaac agtcgggctg tgtcaacgat
     1501 aagagcggct ggctgccgca tcatattatc gtggaaaagg acggcgggct gattgctgcc
     1561 tgcgcgatgt atttaaagct gcatagcatg ggcgagttcg tctttgacta cgaatgggcc
     1621 gaggccgcgc agcacgcggg cattcgatac tatcccaaaa tgctggtcgg tgtgcctttc
     1681 acgccggtga ccggccatcg gttcatcacc gcgcccggcg aagaccggcc gcatttgatt
     1741 cagttcatgg gccaagcgct ggcgaagatc gccgtcgaca acaagatctc gtcggtgcat
     1801 gtcaattttt gccttgccga cgaacgcgag gcgctcgaag cggtgggttt cctgccgcgc
     1861 cacggcattc agtatcattg gcagaatcgc agctttcatt ccttcgacga ttatctcgcg
     1921 agctttcgca gcgatcgccg taataaggtt aagcgcgagc ggcgcgaggt ggccgagcgc
     1981 ggcatcacga tccgcgccta tgagggcgcg gagctgacgc aaaaacagct gcgcacgatg
     2041 tttcgcctgt acaagggcca tatcgaccgg ttgtattacg ggcggcagta tctgacccaa
     2101 gaattttttg acgaactgca caaacgcttt gccgcgcatc tctgcttgat gctcgccgag
     2161 cgcgacggca aaatcatcgc cgggacgttc aacgtgcgcg acgaccaggc gatgtatggc
     2221 cgttactggg gctgcgtcga ggaacatccg tttctccact tcaatgtttg ctactactcg
     2281 gccatcgagc attcgattcg cagcggcctg gaacgtttcg aggccggcgc cggcggcagt
     2341 tttaagcaat tgcgcggtct cgagccggaa cacaccacca gtgttcacta catcgtcgac
     2401 ggacagtttc gcagcgccgt cgaacgtttc ctcacccaag agcgcgagtt gatccggcgc
     2461 aagcgcgaca tgcagttgga acacagccag ttgaagcgcg aacaagaaga accgtaggta
     2521 ggtaatcgcg ttaccgcggt cgcggtgcgt tcaaggttca aaagttaaaa ggttcaaggt
     2581 cggagtagga attgattgcc aagactgctt ccgtcctaaa gcagtgttcc gcgcaggaca
     2641 atggcggcgg cggtgaaata gatcaccaag ccggtgacat cgaccagagt cgcgacaaac
     2701 ggcgcagacg cgctggccgg gtcgaaaccc agcgaacgca ggacaaacgg taagactgag
     2761 cccatcaatg agccccacat caccacgcca actagactta agccgacggt cagggcgatg
     2821 agaaagtaat gctcgccgta ggcgtcaaag aggaattgcc agagaagtat gcgaactagg
     2881 ccgatggcgg cgaggatgct gccgagcgca agcccggttg cgaactcacg gcgaatgacc
     2941 cgccaccagt cgcgcgggcg cacttcgccc aacgccatgg cgcggatcac cagagtcgtc
     3001 gcctgcgagc ccgagttgcc gccgctgctg atgatcaacg gcacgaacag cgcgagaatc
     3061 accgctctgg cgatctcggc ttcgaaatat cccatggccg tcgcggtcag catctcgccc
     3121 aagaagagcg cggcgagcca gccggcccgt ttgcgaatca tcttgggcaa agaaacctgt
     3181 aagtaaggac cgtccaggga ctcgacgccg ccgattttct gaatgtcttc cgtagcctct
     3241 tcttgaacga cgtcgacgat atcgtcgacg ttgacaatgc ctttgatgcg cccggcgccg
     3301 tcgaccacgg gaatcatcaa caagtgatgg cgcatgaata gcttgctcaa agcttcctgg
     3361 tccatgtcgt cgcgcgcgga aatgatttca ctgcgcatca cctcgccaat cgacttgtcg
     3421 gccggagcga cgatcaaatc gcggaaagtg acgacgccga gcaagcgttc atcatggtcg
     3481 acgacatagg cgtaataggt cgtgcgggcg cggctttgag cgtcacggcg caggtaactg
     3541 atggcctcgc cgacggtcat atcggggcga agccgggaat agcgggggtt catcaggccg
     3601 ccggcatcgt cctcggcata atcgagaaga cctttgacct ctcgttgcgt gggatcgtcg
     3661 agcattgcca gcaatgcggc gcgatcttgg gccggagctt cttgaattaa atcggcggcg
     3721 tcgtccagcg cgagcaggcg catccaaagc cggcgttcgg ccggcgtcag accgagcatc
     3781 aagtcggcgc gatcgcgtcc gttcaattgc aggaaaaagg attcggcatc gtcccgttgc
     3841 aagaactcga agccttcgat tttctcggca cgggagagca ccggccaggc atcgtataac
     3901 tcggcgggtg tgaagttgat cgtgccatgg tgatgcactc ttaccggcat gcgctctgct
     3961 cgttcaattt aactcgatcc accgttgcgc gtcgagagtg tcgttatgaa aattatagac
     4021 tcgggcaatt tcttgactcc accgaggctt tccaataacg tcggcgcgga gtgcatctgt
     4081 gaagtcatct tggaaaaatg tcgctgagcc ggtggtcttc tttctcgtcc tattggtctt
     4141 gtgggaaatc ctcgtcgaac tcatcaaggt tccgtcgttt atcttgccgc cgccgcggga
     4201 tttgtggacg gcatttttca aaaaactgcc gatcttgggc catcattcgc tgatcacttt
     4261 cattgaagcc tttggcggct tcgcattgtc actcgttctc ggcgttggtt ttgccatctc
     4321 ggtggtctac tcacgccact tgcaaaacac catctatcca ttgatcgtga ttctttacgc
     4381 catgcccaaa agcgcgtttg cgccgttgat ggttatctgg gtcggctatg gcatgttttc
     4441 gaagatttcc atcgcttttt tggtggcgtt tttccccatc gtcgtcaaca cggttgtcgg
     4501 attgaaagaa gtcgagcccg agctgttgga gctggcgcgc atcaaccgcg gctcggcgtg
     4561 ggatgtcttt aaaaaaatcc gcctgcccaa ctcgctgccc tatatgttcg ccggtatcaa
     4621 ggtcgccatc gtgctgtcgg tcaccggcgc catcgtcgcc gagtttgtcg ccgccaacga
     4681 aggcttggga tatttggtcc tgcaggccaa ctatagtttg gacacggcgt tcgcgctggt
     4741 gatcctgctg attctcgcgg tgttgtcgct cggcctattt tggttggcgg aactctgcca
     4801 aagaaaactg gcgccctgga gcgcggcggt gcgcgaagtc gaaggttcgt tttgaattta
     4861 aaattgcgga ggaaaacgat gaagctttta ctctcaacat tgatggcgtt ggccatagcg
     4921 ctgttcgatt ccggcgctgc gctcgaagca gcggaactga cgccggccaa cgtgcgcatg
     4981 gacttcatca tcggtggcaa gcatgcgccc tggtatgtcg ctttggaaaa gggtttctac
     5041 gccaagcgcg gtttggcggc gacaattcag gcgagcaccg gctcggcgga caccgtgcgc
     5101 accatcggtt ccggcggcgc cgacttcggt ttcgccgatg ccgcgaccat gatcgtggca
     5161 aagtcccgcg gcacgccggt gcaagcggca gcgcagttcg gatatgtcgg cgcgacgata
     5221 ctgtggcgcg acgagggtgt gatcaaaagc atcaaggatt tacccggtaa atcgttggcg
     5281 atcagcccag ggcaggcgca gtggttcctg atgccggctt atgcgcgcat caacaagatc
     5341 gatttcaagg ccatcaaaat tcaggaaacc gcggcgccga tccagcccgc ggctttggcg
     5401 actaagaaag ccgactttat cattatgttc cgggcttcca acgatgaagt tgccgagatg
     5461 gcggcatcga aagtcggtgt caagctcagc cgcgtgttca tgaaagacac cggtctggat
     5521 atttatggca cgagtctgat cgtcaaggat gaagatatca aaaaaaggcc ggaattcgtg
     5581 cgcgcctatg tcgaaggaac cttggaaggg ctgcgatatg cgcgcgacaa tcaggaagag
     5641 gcgcttaaga ttctgctcaa gcataagccc gagctcgatt cggtattgac cacgcagcag
     5701 ctcaaaaacg cgctgaccga agtattcctg ccgttggagt cggtcaatgt cggcatgggg
     5761 ttca
//
LOCUS       DASQKS010000559         4350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_738773, whole genome shotgun sequence.
ACCESSION   DASQKS010000559 DASQKS010000000
VERSION     DASQKS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738773"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VE170_14300"
     CDS             <1..267
                     /locus_tag="VE170_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16664.1"
                     /translation="VIFLLPTFGLHWHWLKVLPTMVPVVKAAIKDEKAQGYFRSFDRQ
                     SYHAHEVGLRDNLVFLVAAVYFAIKGSAAYGVDNWLGAWVVRFF"
     gene            complement(417..1700)
                     /locus_tag="VE170_14305"
     CDS             complement(417..1700)
                     /locus_tag="VE170_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ16665.1"
                     /translation="MKLSAHQLAQQLSASIGADNVIVEKSRLADHAVDTVTPALICAP
                     TTVEQLATVVKVCAAALATMAPWGGGTAIGLGNPPRSLDVVIETTRLHQAIEHDPANL
                     TVTVQSGIALNALQNVIAAERQFVPFDPPLPERSTIGGIVAANLNGPRRSSYGSVRDL
                     VIGLKVVLASGEIIKAGGKVVKNVAGYDMCKLFTGSLGTLGIIAEVTLRVAPLPADTA
                     TASATGTLVQTADLAGQLARTKLLPTATFLLKAGSAPWQLAVSFDGFAETVTRQVDDL
                     GKLAHRLGMTTEIVDGEKPRQQRQAIADLPISENHLVYRLTVPRGAVMQTISTIVDWI
                     KGEPTQTICADMAMGTIWIVLPANRMACELFPRLIALTQQQRGHAVIFNAPAKLKQAK
                     DVWGPATPAHGLMRKIKQEFDPFNLLNPGRFVGSL"
     gene            complement(1697..3010)
                     /locus_tag="VE170_14310"
     CDS             complement(1697..3010)
                     /locus_tag="VE170_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYJ16666.1"
                     /translation="MEPFHHPPETGDKPKIPSLDLAELYQCVHCGLCLNQCPTYRVTR
                     LEAESPRGRIHLVKAAADGRIELNDRFKEHLQLCLLCRACESACPSGVQYGRIAETAR
                     EQIGPPGSPAVKSLLNFVFTQILPHAARLKLAFTLLRLYQSSGLQQLMRLLMPGKLRD
                     LDAMLPAIPSKFFQAPANVLPAIGPRRARVAVLNGCVMPLLFGDVNEATVRVLRRSGC
                     EVIFPTQTCCGALNIHNGEQVVAKQMARRNIDAFLAIGQDAGLDAIVVNAAGCGAAMK
                     EYDFLLRDDTAYAEKAKRFTALVKDAGEFLAGLGLVGALQPINLTVTYQDPCHLAHGQ
                     KIRRQPRELLQAIPGLKLVEMEGSDRCCGSAGIYNLTHAGMSQVLLKDKMQAVGATHA
                     DAVVAPNPGCMLQLRYGAQHYGPNLPVYHLIDLLDRAYGDTLPEP"
     gene            complement(3035..>4350)
                     /locus_tag="VE170_14315"
     CDS             complement(3035..>4350)
                     /locus_tag="VE170_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYJ16667.1"
                     /translation="IVVVPANVEEVSAVVKAAQQANVPIVPRGSGTGLAGGAVPAEGG
                     IILSLSRLDRILTIDLENRFAVVEPGVINLDVTKAVAKDGYFYAPDPSSQAACSIGGN
                     VANNSGGPHTLAYGVTTNHVLGIEVVLDDGEIVWLGGEVPDTPGYDLCGVFVGSEGTM
                     GIVTKIAVKLMPTRESVGTLLAIFDDMDFATKTVVDITAAGIIPAALEIMDQTAIEAV
                     ERGAPVGFPRDAAAVLIVEVEGLSEHTEPSIKLASEVCQRNGAREVRVAQDEAQRQLL
                     WKGRKGAFGAMGTLAPNYYVQDGVVPRSQLPEMMRRVAAIGKEFNLTIANVFHAGDGN
                     LHPNILFDMRKPGELARVIAAGAATLRACVELGGSITGEHGVGMEKKAYIGLLFNDRD
                     LAAMTRVRHAFDPDNRFNPAKLFPTPASCGEVRLEPAKIPAGLWI"
BASE COUNT          855 a         1212 c         1334 g          949 t
ORIGIN      
        1 gtgatctttt tgctaccgac ctttgggctg cattggcact ggcttaaagt cttgccgacc
       61 atggtgccgg tggtcaaggc ggcaatcaaa gatgaaaaag ctcaaggcta ctttcgttcc
      121 ttcgaccggc aatcatatca tgcccatgaa gtcggcctgc gcgacaactt ggtgtttcta
      181 gtcgcggcgg tctacttcgc catcaaaggc agcgcagctt atggtgtcga caactggttg
      241 ggcgcttggg tagtgcggtt tttctaaatc gttggcttcg gaatggaata tggccgcaaa
      301 agacgcaaag tctgaaaaaa acaatcagac atgcaggagc cggttaaaaa ctcgccctct
      361 ttaggttggg caagcagatc gttcaggaga ttcgcattgg ccaggcaacc ttaagcttac
      421 aggctgccga cgaagcggcc gggatttaac aggttaaacg gatcgaattc ctgcttgatt
      481 tttcgcatta gtccatgggc gggtgtcgcc ggaccccaga catccttggc ttgcttcaac
      541 tttgccggtg cgttgaagat tacggcgtga ccgcgctgct gttgggttag tgcgattaga
      601 cggggaaaca attcacacgc cattcgattc gccggcaaga cgatccagat cgttcccatt
      661 gccatatcag cgcagatcgt ctgtgtcggt tctcctttta tccagtcgac aattgtcgag
      721 atcgtttgca tgaccgcacc gcgtgggacc gtgaggcgat agaccaaatg attttcgctg
      781 atcggtaggt cggcgatcgc ttgccgctgt tgccgtggtt tttctccatc gacgatttct
      841 gtagtcatgc caagtcggtg cgccaacttg cctagatcgt cgacctggcg cgttacagtt
      901 tcggcaaagc catcgaaact gaccgctagt tgccaaggcg ccgaacccgc cttgaggagg
      961 aaggtcgcgg tcgggagcaa tttggtgcgc gccaactgcc cggctaaatc cgccgtttgg
     1021 accagtgtgc cggtggcgct ggcagtcgcg gtatcggccg gcagcggcgc tactcgtaag
     1081 gttacttcgg cgatgattcc caaggttccc agtgaaccgg tgaagagttt gcacatgtcg
     1141 tagccggcga cgttcttgac gaccttgccg ccggctttga taatctcgcc tgaagcgaga
     1201 actactttca ggccgatgac caggtcgcgt acgctgccgt agctgctgcg ccgcggcccg
     1261 ttcaaatttg ccgcgacgat gccgccgatg gtcgagcgct ctggcagcgg tggatcgaag
     1321 ggtacgaact gccgttccgc agcgataacg ttttgcagcg cgttgagggc aatgccgctt
     1381 tgcacagtta ccgtgagatt ggcaggatca tgttcgattg cttgatggag ccgagtggtt
     1441 tctatgacga catcgaggga ccgcggcgga ttccccaaac cgatcgccgt ccccccaccc
     1501 cacggcgcca tggttgccag cgctgccgca caaactttta caacggtggc taactgttcc
     1561 acggtcgtgg gtgcgcagat cagtgctggt gtgacagtat cgaccgcgtg atccgcaagc
     1621 cgacttttct cgacgatgac attatcggcg ccgatgctcg cggatagctg ctgggcaagc
     1681 tgatgcgcag atagtttcat ggttccggca gcgtgtcgcc ataggcgcgg tcgagcagat
     1741 cgatcaaatg atagaccggc agattcggtc cgtagtgttg cgcgccgtag cgtagttgga
     1801 gcatgcagcc ggggttgggc gcgaccaccg cgtcggcatg agtcgcgccg acggcttgca
     1861 tcttgtcctt gagcagcacc tgcgacatgc ctgcgtgggt taagttgtaa atcccggcgc
     1921 tgccgcaaca gcggtcacta ccctccattt caactaattt aagtccgggg atcgcttgca
     1981 atagctcacg tggttggcgg cgaatctttt gcccatgggc gaggtggcag ggatcctggt
     2041 aagtgacggt tagattgatc ggctgcaatg cgccgactaa accgagcccg gcgagaaatt
     2101 cgccggcgtc tttgaccaga gcggtgaagc gtttggcttt ctcggcgtag gccgtatcgt
     2161 cgcgcaggag aaaatcatat tccttcatcg ccgcgccgca gccggcggca ttgacgacga
     2221 tggcatccaa gccagcatct tggccgatgg cgagaaaggc gtcgatatta cgccgcgcca
     2281 tctgcttggc gactacttgt tcgccgttgt gaatattcaa agcgccacag caggtttgcg
     2341 tcgggaaaat cacctcgcaa ccgctgcgcc gcagcaccct taccgtggct tcgttcacgt
     2401 cgccgaagag cagcggcatg acgcaaccgt tgagcacggc aacgcgggcg cggcgcggac
     2461 cgatggcggg tagaacgttc gccggcgcct ggaaaaactt cgacgggatc gccggcaaca
     2521 tggcatcgag atcgcgaagt ttcccaggca tcaacaggcg catgagttgc tgcaggccgc
     2581 tactctggta caggcgcaaa agcgtgaacg cgagctttaa ccttgcggcg tggggcagta
     2641 tctgcgtaaa gacgaagttc aatagcgatt tgaccgccgg cgaacccggc ggaccgatct
     2701 gttcgcgagc ggtttcggcg atccggccat actgcacgcc ggagggacaa gcggactcgc
     2761 aggcgcggca gagcagacaa agttgcaggt gttctttgaa gcgatcgttc aactcaatgc
     2821 gtccgtcggc ggcagctttg accagatgga ttctgccgcg cggcgattcc gcttccagac
     2881 gcgtgacgcg gtaggttgga cactgattca agcacaggcc gcagtgaacg cattggtaga
     2941 gttcggcgag gtcgaggctc gggatttttg gtttatcccc ggtctcggga ggatggtgga
     3001 aaggttccaa gtgatcgctt ctttagttgt aagcctagat ccacaagccg gctggaattt
     3061 ttgccggttc taggcgcacc tcgccacagc tcgccggggt cgggaaaagt tttgccggat
     3121 tgaagcgatt atccgggtcg aaggcatgac gcacccgggt catggcggcg agatcgcggt
     3181 cgttgaatag caagccgatg taagcctttt tttccatgcc gacgccatgt tcgccggtga
     3241 tactgccgcc cagttcgacg caggcgcgca gggtcgccgc gccggctgcg atgacgcgcg
     3301 ccaactcgcc gggtttccgc atgtcgaaca agatattcgg gtgcagattg ccgtcgccgg
     3361 catgaaacac gttggcaatg gtcaagttaa attctttgcc gatggcggcg acgcggcgca
     3421 tcatctcggg tagctggctg cgcggcacga cgccatcttg gacgtaatag ttgggcgcca
     3481 acgtgcccat ggcgccgaat gcgcctttgc ggcctttcca taacagctgg cgttgggctt
     3541 catcctgggc gacgcgcact tcccgggcac cgttgcgctg gcacacttca ctggccagct
     3601 ttatcgacgg ttcggtatgt tcgctaagcc cttcgacttc gacgatcagc accgccgcgg
     3661 cgtcgcgtgg aaagcccacc ggagcgccgc gttcgacggc ttcgatggca gtttgatcca
     3721 tgatttccag cgccgccgga ataattccgg cggcggtaat gtcgacgacg gttttggtgg
     3781 caaagtccat gtcgtcgaag atcgccagca gggtacctac tgattcgcgg gtcggcatga
     3841 gtttgacagc gattttcgtg acgatgccca tggtgccttc ggagccgacg aaaacaccgc
     3901 agagatcgta gcccggcgtg tcggggactt cgccgcccag ccagacgatt tcgccatcat
     3961 ccaggacgac ttcgatgcct agtacatgat tggtggtaac gccataggcc aatgtgtgcg
     4021 gcccgccgga attattcgcc acgttgccgc cgatggaaca cgcggcctgg ctgctcggat
     4081 cgggagcgta gaaatagccg tctttggcga cggctttggt gacatcgaga ttgatgacgc
     4141 cgggttcaac gacggcgaat cgattttcca gatcgatagt gagaattcga tccaatcgcg
     4201 atagcgacaa aatgatgccg ccttcagccg gcacagcgcc gcccgccaga ccagtgccgg
     4261 agccgcgcgg tacgatcggc acattggctt gctgcgccgc tttgaccacc gctgagactt
     4321 cttcgacgtt ggccggcacg acgactattt
//
LOCUS       DASQKS010000560         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1165472, whole genome shotgun sequence.
ACCESSION   DASQKS010000560 DASQKS010000000
VERSION     DASQKS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165472"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VE170_14320"
     CDS             complement(<1..340)
                     /locus_tag="VE170_14320"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16668.1"
                     /translation="MLVLKCWSRALIPALALLLLPSAGRAQMKARVAWTSFASNMSGT
                     WVAQEEGLFRKNGLEVELVHIPSTSRAIQVMLAGELHYSYMDGRNSVTAALKGADVAI
                     IAGVANRLVFS"
     gene            complement(510..1508)
                     /locus_tag="VE170_14325"
     CDS             complement(510..1508)
                     /locus_tag="VE170_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16669.1"
                     /translation="MRDVTIVVGTIGQSIMRSEDNGQTWARIGPRRGFAYEASVRCIA
                     MNPKQHNILFAGTERGLYRSADGGANWQYLDSALNPHYVWAIGIDPVNPDNIFVGTGT
                     PTPAMLFRSKDGGRSFDKRPVEVATECEAVGTPRFTGIAVDPVEPNHIWAGLEVDGAR
                     HSSDGGDTWNIVEVTGRRDIHNVAVTAGPPKTAFIMANREIYASTDNGKSWEPRDMQK
                     CLPWEYPRKENYLRGIAVDPANPKKIMVSFGDFTPGTSGAIAQSDDFCKSWKLLPLPQ
                     EPNSTMWTFATNSDDPKVMFAASRYGYLYRTDDGGASWTKLRRELSEIAAVCWLPN"
     gene            complement(1549..2529)
                     /locus_tag="VE170_14330"
     CDS             complement(1549..2529)
                     /locus_tag="VE170_14330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ16670.1"
                     /translation="MDYVQFGSTEFKVSRLALGCMSMSGAYGPADDNESIATLHKAFD
                     LGINFLDTSASYGQGHNHELINKALQGRRERIVIHSKSGSPRAPDAAGNRSGSTPEYL
                     TQNCEQSVKRLGIDALDIFCMSRVDPDIPVEESVGAMAKLVERGLTRYIGLSEASANS
                     IRRARKVHPIVSLQMEYSLWSRDPEGGNLEACRENDMGFMAYSPLGRGFFGGAVHDAK
                     DMSEGDGRINHPRFQGDNLAKNLALLARFEELAREKHCTPAQLALAWLMAQGSGVIPI
                     PSNKTRAHLEENLKATEIKLTKEDLARLDAIFPPGVAAGPRTKDMHRVNI"
     gene            2667..2891
                     /locus_tag="VE170_14335"
     CDS             2667..2891
                     /locus_tag="VE170_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYJ16671.1"
                     /translation="MSSSIETIEAEALRLSPTDRARLVERLIASLDVDPEIEEAWATE
                     VERRNSEVESGTVSLVSGPEAIAKLKALFK"
     gene            2917..3180
                     /locus_tag="VE170_14340"
     CDS             2917..3180
                     /locus_tag="VE170_14340"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYJ16672.1"
                     /translation="MDLREAAEFYQKRAGNDLALTFIAEFEYSVSLLLDHPTLGNAWR
                     NGRRRFVMRRFPYSLIYDTVDQQIQIIAVAHHSRRPGYWQDRN"
     gene            complement(3218..>3855)
                     /locus_tag="VE170_14345"
     CDS             complement(3218..>3855)
                     /locus_tag="VE170_14345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16673.1"
                     /translation="RGDAPFKTVADVRKAAEGPKCGSTGTGTTSHYLPTLLNDGIGTK
                     FHVVTGYPGGPEIDLAVERNEVQCRSFTIAGWFTGDLYANWRKTGFARAVVQSHRKRD
                     ARLADVPTVYELLDEFKAPEQIKKLASLLIASNSFGRPYVLPPATPPEYVKILRESFM
                     RTLNDPELLADAKRRRIDIEYTSGEELDRLAKEVITKDTEVIERMKKLLGK"
BASE COUNT          784 a         1188 c         1062 g          821 t
ORIGIN      
        1 acgagaacac caagcgattg gcgacgccgg caataatcgc tacgtcggcg cctttgagcg
       61 ccgccgtcac cgaattgcgc ccgtccatgt aagaatagtg aagttcgccg gcgagcatca
      121 cttgaatcgc ccgcgaagtc gacggaatat gcaccagctc gacttcaagg ccgttcttcc
      181 ggaacagccc ttcctcctgc gccacccacg tgccgctcat gttgctggcg aacgaagtcc
      241 acgccacccg cgccttcatc tgcgccctgc cggcgctcgg taagagcagc aaagccagcg
      301 caggaatcaa cgcacgggac caacatttta atacaagcat cttcggcatt cctggccttt
      361 tcataaatag cgatttagcc gcaaagaacg caaaggcaca aaaccgcatg aacaatccgc
      421 agcgaagggc agcctccccg ggctgggaat ccggccttga accttgaact tttgaaccct
      481 tgaaccgctc ggcgcgaaaa gcgccgcgct caattcggca accagcacac cgccgcaatc
      541 tcgctcagct ctctgcgcag cttggtccat gacgcgccgc cgtcatcggt gcggtagaga
      601 taaccgtagc ggctcgcggc aaacataacc ttcgggtcgt cggaattagt cgcgaacgtc
      661 cacatggtcg agttaggttc ctggggcagc ggcaagagct tccagctctt acagaaatca
      721 tcgctttgcg cgattgcgcc tgaggttccc ggcgtgaagt cgccgaagct caccatgatt
      781 tttttcggat tggccgggtc gacggcaatg ccgcgcagat agttttcttt gcgcggatac
      841 tcccacggca aacacttctg catgtcgcgc ggctcccagc tcttgccatt gtccgtgctc
      901 gcatagatct cccggttggc catgatgaat gccgtcttgg gcgggccggc ggtgaccgca
      961 acgttgtgaa tatcgcgccg cccggtcacc tcgacgatat tccaggtgtc gccgccgtcg
     1021 ctgctgtgac gggcgccgtc cacttcgaga cccgcccaga tatgattggg ctcaaccgga
     1081 tcgacggcga tgccggtgaa cctcggcgtg cccaccgcct cgcattccgt cgccacttcc
     1141 accggccgct tgtcgaagct gcggccgcca tccttcgaac gaaagagcat ggccggagtc
     1201 ggcgtgccgg tgccgacaaa aatgttatcc ggattcaccg gatcaatgcc gatcgcccag
     1261 acatagtgcg gattgagcgc cgagtcgagg tactgccaat tggcgccgcc gtcggcgctg
     1321 cgataaagcc cgcgctcggt accggcaaag agaatattgt gctgcttcgg attcatcgcg
     1381 atgcagcgca ccgaagcctc gtaggcaaat ccccgccgcg gcccgatgcg cgcccaagtc
     1441 tggccattgt cttcgctgcg catgatgctt tgtccgatag ttccgacaac gatagttaca
     1501 tctcgcatat gtcctcccaa gacgacttga acgttttgaa cgagtcttct agatgttgac
     1561 ccgatgcata tccttggtcc gcggtcccgc cgccactccc ggcggaaaga ttgcgtcgag
     1621 ccgggctaaa tcttctttag tcaatttaat ctccgtcgct ttgaggttct cttccaaatg
     1681 ggcccgcgtc ttattgctgg ggatcggaat cacaccgctg ccctgagcca tcaaccacgc
     1741 cagcgccagc tgcgccggcg tgcaatgttt ctcccgcgcc agctcttcga agcgcgccag
     1801 cagcgccaga ttcttcgcca gattgtcgcc ttggaatcgc ggatggttga tccggccatc
     1861 gccctcgctc atgtctttgg cgtcatgcac ggcgccgccg aaaaagcccc ggcccagcgg
     1921 cgagtacgcc atgaagccca tgtcgttttc gcggcacgcc tcgaggttgc ccccttcggg
     1981 atcccgcgac cacagcgagt attccatctg caatgacact atcggatgaa ccttgcgcgc
     2041 gcgccggatc gaattggcgc tcgcttccga caagccgatg taacgcgtca gaccgcgctc
     2101 gaccaacttc gccatcgcgc ccaccgattc ctcgacgggg atatccgggt ctacgcgcga
     2161 catgcagaaa atatcgagag catcgatgcc caaccgcttg acgctttgct cgcaattctg
     2221 ggttaggtat tccggcgtgc tgccgctgcg attgccggcg gcatccggcg cccgcgggct
     2281 gccggatttg gagtgaatca cgatgcgttc gcgccgcccc tgcagcgcct tgttgatcaa
     2341 ctcatggtta tgaccctggc cgtagctcgc cgaggtatcg agaaaattga tgccgagatc
     2401 gaacgccttg tgcagcgtcg cgatcgattc gttgtcgtcc gccggaccat aggcgccaga
     2461 catactcatg cagcccaaag ccaaccgcga gactttgaat tccgtcgagc cgaattgaac
     2521 gtaatccatg ctgcctccgt actgaagtca gagttaccgc aggtggacgc aagaagtcaa
     2581 acttttgacc gaacgaaatc acgggtttga acccaatttg tcgatatggc agtctgttgc
     2641 tgtattgtcc ggatttggag gtctgaatgt cttcatcaat tgaaacgatt gaagccgaag
     2701 cgttacggct ttcaccaact gacagagcac gattggttga acgtctgatt gccagtcttg
     2761 atgtcgatcc ggagatcgag gaagcatggg ccactgaagt cgaacggcgt aacagtgaag
     2821 tcgaaagtgg aacggtatcc ttagtctctg gtccagaagc gatcgctaaa cttaaagcgt
     2881 tgttcaaatg accatcgcct gcaccccgat gcggaagtgg atcttcggga ggcagctgaa
     2941 ttctatcaaa agcgcgcggg caatgacctc gcgctgactt ttatcgccga gttcgaatac
     3001 tctgtaagcc tccttcttga ccacccgacg ttgggcaacg cttggcgaaa cggtcgacgc
     3061 cgctttgtta tgagacgttt tccatactcc ttgatttacg atacggtgga tcagcaaatc
     3121 caaatcatcg cggttgccca ccacagtcga cgtcctggat attggcagga tcgcaattag
     3181 cagagctgat acagattcac ctctagcgcg tttcgcctca cttccccaac aacttcttca
     3241 tccgttcaat cacttcagtg tctttggtaa tcacttcctt agcgagccga tcgagctcct
     3301 cgcccgaagt gtattcgatg tcgatgcggc gccgcttcgc gtcggccaag agttccggat
     3361 cattgagcgt gcgcatgaag gattcgcgca ggatctttac gtactcgggc ggcgtcgccg
     3421 gcggcagcac atagggtcgg ccgaaagaat tggaagcgat cagcaagctc gccagcttct
     3481 tgatctgctc cggcgctttg aactcatcga gcagctcata gactgtcggc acgtcggcga
     3541 ggcgcgcatc gcgcttgcga tgactctgca ccacggcacg ggcaaagccg gtcttgcgcc
     3601 aattggcgta gagatcgccc gtgaaccaac cggcgatggt gaaggagcgg cactgcactt
     3661 cgttgcgctc caccgccaga tcgatttccg gtccgcccgg atacccggtg acgacatgga
     3721 acttcgtgcc gataccgtcg ttcaatagcg tcggcagata gtggctggtt gtgccggtgc
     3781 cggtcgagcc gcacttggga ccttcggcag ccttgcgcac gtcagccaca gtcttgaacg
     3841 gcgcgtcgcc gcgca
//
LOCUS       DASQKS010000561         5567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1978164, whole genome shotgun sequence.
ACCESSION   DASQKS010000561 DASQKS010000000
VERSION     DASQKS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978164"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..776)
                     /locus_tag="VE170_14350"
     CDS             complement(54..776)
                     /locus_tag="VE170_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ16674.1"
                     /translation="MAASGYTGPAFDSSELKELKAKVVNACLILDREGITDGFGHVSA
                     RIPEQSAFLTIAQVSPGCAALDKVVLLDLDGNFLGGAKPAPYEWPIHACIMRARPDVL
                     SVCHTHSKWSALFSVLKKGLRPAHMYGRFLPVSGAPIYPEAGLITSVERGQALADTLK
                     DGAATLLRAHGDAVVGASVEQAILRTVQLAFLGELAHLAAVHGGPDYLTEEELAIFAG
                     DRSFPARPWEYFLSRLGERRVA"
     gene            1007..1477
                     /locus_tag="VE170_14355"
     CDS             1007..1477
                     /locus_tag="VE170_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HYJ16675.1"
                     /translation="MLRITYQVTGNIDKLKIPANSSPRRTDRLWQHTCFEAFIRADSD
                     PSYYECNFSPSGEWAAYSFRSYRDGAPIRDESCSPEIVVRRETDRIELDAVVRLNRLP
                     GIRLGSLLHIGLSAVIEAKDGTLSYWALKHPVAKPDFHHSDSFALELPFPGQCR"
     gene            1498..2700
                     /locus_tag="VE170_14360"
     CDS             1498..2700
                     /locus_tag="VE170_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYJ16676.1"
                     /translation="MKFGIDRLLDDIALRKPLAGMRVALLAHPASVTRDLVHSLDALA
                     ALPDIKLSAAFGPQHGLRGDKQDNMVESADFLDPVHGIPVFSLYGAVRRPTAAMMDSF
                     DVLLVDLQDVGCRIYTFITTLRYVLEAAAHHKKSVWVLDRPNPVGRPVEGLQLRPGWE
                     SFVGAGPLPMRHGLTLGELAQWFVQTSKLDLDCQVIQMEGWNPAAAPGYGWPLGERSW
                     INPSPNAANLSMARCYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDPHALLQKMAALA
                     PDWLRGCRLRECWFEPTFHKHTGRLCAGIQIHVDDPSYEPNAFKPWRLMALAFKALRL
                     LQPEYPLWRDFAYEYEKDRLAIDLINGSDLLRMWVDDLTATIGDLDILATPDEVSWST
                     KIKSVVLY"
     gene            complement(3046..4035)
                     /locus_tag="VE170_14365"
     CDS             complement(3046..4035)
                     /locus_tag="VE170_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16677.1"
                     /translation="MKTLPIALLLVLTNFVSNCPAAEKLVAGYSSISPSELTLITASK
                     TGLFEKYGLDVSTVYLGGSTRIVQAMISGDVQIGQIGGSAVLFGKAAGIDTVFIATII
                     NSMAAQIMSRPEIKQTSDLKGKAVGVTRRGANTDYWARFGLSHHGLVPDKDVKIIYTG
                     GLRETYTALQAGQIAAAAMGLGNSFAQLLSRQGYNTVIDVSKLGGDFPFNGVATTRRF
                     MQTKRPIVVSFMKAYLEAIKLNLENKVLSKKLLASHGGINDDEILEIAYDIYVNKIRA
                     KIPYPVNRGWKTLIDFTARDNPKVKEVKAEEILDDSIVKELDESGFVKSLGLK"
     gene            4162..4401
                     /locus_tag="VE170_14370"
     CDS             4162..4401
                     /locus_tag="VE170_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16678.1"
                     /translation="MEHGKLRHIAFISQLPKVQSDFYQKYFRFEECKVFPSGARMVID
                     RFLISPSCSKQQSAAVSGRIEPTISYFLSAAAHGT"
     gene            4391..>5567
                     /locus_tag="VE170_14375"
     CDS             4391..>5567
                     /locus_tag="VE170_14375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16679.1"
                     /translation="MGRKHKFASFFTECVLTMPLFQRGYETRGKDGGQVCAHIVLEKL
                     VNSAALAYGPAMGYGLFYLPSSLPATRAEGAERFRTVIEQVAYGEKIGFESVWLAEHH
                     FHPFAGIFSAPPVIGAAIAQRTEKMRIGTAVLLLPYHNPLRVAEDYATLDCLSNGRVE
                     FGVGHGFVKWEALTFGIPLDELRDRFRENLEVVLKAWRDPKLEHKGRFCEYHGVEVLP
                     RPVQEPYPTVWMAATTSVESFEYSGAQGFHMMLIPFLNEIEELREKMQTYFNARRAAG
                     HDPASARVLGVYHAYVGENPAEARAAAAHGLHEYHSAAQTAHNLTPGMADPESYRSHD
                     KHRTAMKRLTFDELVDQNRVLVGSAPEVREKVEYVRERLYLTDLAGNFALGGLSDQQA
                     "
BASE COUNT         1148 a         1525 c         1622 g         1272 t
ORIGIN      
        1 atgcgcgaac aatcaattta ttgtcgggac aatagttgcc ggtcatctgt ctgtcaagca
       61 acgcgccgct cgcccagccg gctcaaaaaa tattcccatg gacgcgctgg aaacgaccgg
      121 tcgccggcga atatcgccag ttcctcttcg gtcaggtaat cgggcccgcc gtggaccgcg
      181 gccaagtgtg ctagctcccc gagaaatgcg agctgcacgg tgcgcaggat cgcctgctcg
      241 acgctggcgc cgacgacggc gtcgccgtga gcgcgcagta aagtggcggc gccgtctttc
      301 aaggtgtcgg ccaacgcctg gccccgctca acggaagtga tcaagccggc ttcgggatag
      361 atcggcgcgc cgctaacggg aagaaacctg ccatacatat gcgccggccg gagccctttt
      421 tttaacacgc taaaaagcgc gctccatttg gagtgagtgt ggcacacgct caggacgtcc
      481 gggcgcgcgc gcatgatgca tgcatgaatc ggccactcgt agggcgccgg tttagcgccg
      541 ccgagaaagt tgccgtcgag gtcaagtaga accactttgt ccagcgccgc gcagccgggg
      601 ctgacctggg cgatggtgag aaaagccgac tgttccggaa tccgcgcgct aacatggcca
      661 aagccgtcgg tgataccttc gcggtcaaga atgagacaag cattgacgac cttcgccttg
      721 agttctttga gctcgctaga atcaaatgcc ggtcctgtgt aaccactcgc tgccatcggt
      781 actccttggt tattcgatta ttagtttcct ggttctatgc gcgggcggac ggggtgtcaa
      841 ggagagtctt cgcaggctgg tgtaatggct cgcaatcggc taatttccgt aacgcacaag
      901 gcaatcctcg gggatgaaca tctcgacaac tacgctatta ccatttgtgc ttggccacgg
      961 cggcgcgaga tcaattcacg cttgcgtgaa tggtgccgat gccaacatgc tgcggatcac
     1021 ttatcaggtg accggtaaca tcgacaagtt gaagatcccg gcaaattctt ccccccgccg
     1081 gaccgatcgt ctttggcagc atacttgttt cgaggcattc atccgtgccg atagcgatcc
     1141 gtcgtactat gaatgcaact tttcgccctc aggcgagtgg gcggcgtact cgtttcgcag
     1201 ctatcgcgat ggcgccccta tacgagatga aagttgctcg ccggaaatcg tggttcgccg
     1261 tgaaacagat agaatcgaac tcgatgccgt cgtgcgcttg aatcgtttgc cggggattcg
     1321 actcggatcg ctgctgcaca tcggcttatc ggcggtgatc gaggccaagg atggcacgct
     1381 ttcctactgg gcactgaaac atcccgtggc gaagcccgac tttcatcact ccgattcatt
     1441 cgcattggag ctgccttttc ctggccagtg cagatgaaag cgcctaaaat agactttatg
     1501 aagtttggca tcgaccgact gttggacgat atagcgctac gcaagccgct cgccggcatg
     1561 cgcgtggctt tactggcgca tccggcgtcg gtgacacggg atctggttca ttcgctggac
     1621 gcgttggcgg cgttaccgga catcaagctc agtgctgctt tcggaccgca gcatggtctg
     1681 cgcggcgaca agcaggacaa catggtcgag tcggcggact ttctcgatcc ggtgcatggc
     1741 attccggtct tcagtttgta cggcgcagta cgccggccga cggcggcgat gatggatagc
     1801 ttcgatgtgc tgctggtgga tctccaagac gttggttgcc gcatttatac tttcatcacc
     1861 acgctgcgct atgttctcga agctgccgcg catcacaaga aatccgtctg ggttctcgac
     1921 cggcccaatc cggtggggcg gccggtggaa ggattacagc ttcgcccagg gtgggagagt
     1981 tttgtcggcg cgggtccatt gcccatgcgc catggattga cgttgggcga attggcgcag
     2041 tggttcgttc aaacatcgaa gctcgatctc gattgccaag tcatccagat ggaaggatgg
     2101 aatccggcgg cagctccggg ctacggctgg ccattgggtg aaagaagttg gatcaatccc
     2161 agtcccaacg cagctaattt atcgatggcg cgctgctatg ccggcacggt catgctcgaa
     2221 ggcacgacac tctcggaagg acgcggcacg acccggccgc tggagctctt cggcgcgccg
     2281 gatatcgacc cccacgcgct gttgcagaaa atggccgccc tcgcgcccga ctggctgcgc
     2341 ggttgccgtc tgcgcgagtg ctggttcgag ccgaccttcc acaaacacac gggcagactc
     2401 tgtgccggca tccaaatcca cgttgacgat ccgagctacg agcctaatgc tttcaagccc
     2461 tggcgtttga tggcactggc gttcaaagcg ctgcgcttgt tgcaaccgga gtatccgctc
     2521 tggcgcgact tcgcgtacga gtacgagaaa gaccgtctcg ccatcgatct aattaatggt
     2581 agtgacttgc tgcgaatgtg ggtggatgac ctgacagcaa caataggcga ccttgacatt
     2641 ctcgcgacgc cggacgaagt gtcttggagc actaagataa aatcagttgt cttgtattga
     2701 agactttcgt tcgaagtatc ggctggttgc ttcgattgtt acggttgtta tttcttcacg
     2761 gcgtctatca atcgacttgt caatccccca aacccgcgac cgaaagtacc agttccgaaa
     2821 gacgagcttt ctcagtacgt agtagatcga accggagtct cgctggatgc agaattatcg
     2881 gaccattacg aagatccgta agaatcaatt tgtgagagat catcgctgag gctttctaac
     2941 gtgatggcca aaataatcat cagtgatagc gcgttttcac taagcattga tttcaagggc
     3001 tgtagggtgg gaaatgacta tcgattgtaa ggttggaggc gctatctatt tcagccccag
     3061 actcttcaca aacccgcttt catccaattc cttcacgatg ctgtcatcga gaatctcttc
     3121 tgccttcacc tccttaactt tcggattgtc tctcgcggtg aagtcgatca gggttttcca
     3181 gccgcggttg accgggtagg ggattttggc gcggattttg ttaacgtaga tatcgtaggc
     3241 aatttccaaa atttcgtcgt cgttgatgcc gccgtggctg gcgagcagct tcttcgacag
     3301 cactttattc tccaaattca atttgattgc ttcgagatag gccttcatga agctgacgac
     3361 tatgggtcgt ttggtctgca tgaagcggcg cgtggtggcg acgccgttga acgggaaatc
     3421 gccgccgagt ttggagacgt cgatcacggt gttgtagcct tggcgtgaca gtagttgcgc
     3481 gaaggaattg ccgaggccca tcgccgcggc ggcaatttga ccggcttgca gtgccgtgta
     3541 agtttcccgc aggccgcctg tgtagatgat cttcacgtct ttgtccggca ccaggccgtg
     3601 atggctcagg ccgaagcggg cccaatagtc ggtgttggcg ccgcgtcttg tgacgcctac
     3661 ggctttgcct tttaaatcgc tcgtctgttt gatctcaggg cgcgacatga tttgcgccgc
     3721 catgctgttg atgatggtgg cgataaagac tgtatcaatc ccggcggctt tgccgaagag
     3781 cacggctgag ccgccgatct ggccgatttg cacgtcgccg gaaatcatcg cctggactat
     3841 tctcgtgctg ccgccgagat agacggtgct tacgtcgaga ccgtattttt cgaacaggcc
     3901 agtcttagac gcggtgatta gcgtcagctc cgagggactt atggatgaat agccagcgac
     3961 cagcttttcc gccgccgggc aatttgagac aaagttggtg agtaccagca ggagcgcaat
     4021 tggcagagtc ttcatcgggc aactccggaa ccgagatatc agaaccgacg cgccaccgtc
     4081 aattgctttg ttgaccagac aaaatccgcg ttgacgccaa ctcgttaccg cactattggc
     4141 gccgctagat gatcactgga ggtggaacat ggcaagctca gacatatcgc gtttatttct
     4201 cagctgccga aggtacaaag cgatttctac cagaagtatt tccgcttcga ggaatgcaaa
     4261 gtcttcccca gcggcgcgcg catggtgatc gaccgctttt taatctcgcc ttcttgcagc
     4321 aagcagcagt ccgcggcggt ctccggacgc atagagccga cgatatcgta ctttctttcc
     4381 gcagcggccc atgggacgta aacataagtt cgcttctttt tttaccgaat gcgttttgac
     4441 aatgccgcta tttcaacggg gatacgaaac tcgaggcaaa gatggcgggc aggtgtgtgc
     4501 tcatatcgtc ctcgaaaaac ttgtcaattc cgccgccctg gcatatggtc cggccatggg
     4561 ttacgggctt ttctatttgc cgtcgtcgct gccggcaacc cgcgccgaag gcgccgaacg
     4621 atttcgcacg gtcatcgagc aagtagccta cggcgaaaag atcggttttg aatcggtttg
     4681 gctcgctgag catcactttc atccgtttgc cggcattttt tccgcgccgc ccgtgatcgg
     4741 cgcggcgatc gcgcagcgga ccgaaaagat gcgcatcggc accgcggtac tgctgttgcc
     4801 ctatcacaac ccgttgcgtg tcgcggaaga ttacgcgacc ctcgattgct tgagcaacgg
     4861 ccgggtggaa ttcggcgtcg gccatggctt tgtcaagtgg gaagcgctga ccttcggcat
     4921 tccgctcgat gagttgcgcg atcgttttcg ggaaaatctc gaagtggtcc tcaaagcgtg
     4981 gcgcgacccg aagctcgaac acaaagggcg cttttgcgaa tatcacggcg tcgaagtgct
     5041 gccgcgcccg gtgcaggagc cgtatccgac ggtgtggatg gcggcgacga cttcggtgga
     5101 atctttcgaa tactcgggcg ctcaaggctt tcacatgatg ttgatcccgt ttttgaacga
     5161 gatagaggag ctgcgcgaga agatgcagac ctatttcaac gcgcgccgcg cggccggaca
     5221 tgatccggcc agcgcgcggg tactcggcgt gtatcacgcc tacgttggcg agaacccagc
     5281 tgaagcgcgc gccgcggctg cgcatgggtt gcatgaatat cattcggcgg cgcagacggc
     5341 gcataatttg acacccggga tggccgatcc cgagtcatac cgcagtcacg ataaacatcg
     5401 gacggcgatg aagcggctga cgttcgacga attagtggat caaaaccgcg tgctggttgg
     5461 cagcgcacca gaggttcgcg aaaaagtaga atacgtccgt gagcggcttt atttaaccga
     5521 cctggccggc aacttcgcat tgggcgggct aagtgaccaa caagcgc
//
LOCUS       DASQKS010000562         4244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2846743, whole genome shotgun sequence.
ACCESSION   DASQKS010000562 DASQKS010000000
VERSION     DASQKS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846743"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VE170_14380"
     CDS             complement(<1..641)
                     /locus_tag="VE170_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYJ16680.1"
                     /translation="MSRVYLNQLGMISPLGNSVDATKQRLLELVQSGVALSDVYSPGR
                     LLPVGRIDADFTLPPMDAWPLPDRSRNNQIVLAALQQIRPAVQSAIERFGGARVGVVI
                     GTSTSGIGEAEAALRDYLSTGALPQGFHYGQQELVSPAAVLATELGICGPAYVHSSAC
                     ASSAKALASAARLIGMNVCDAVLAGGVDTLCRFTVAGFAALESVSMTRCNPLSR"
     gene            complement(638..1198)
                     /locus_tag="VE170_14385"
     CDS             complement(638..1198)
                     /locus_tag="VE170_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HYJ16681.1"
                     /translation="MLPGLSRGGALLAALAVHSFGCADAPALPARLGLKLAPATLGVS
                     LSLQQHLRIERQGRVDELDAALEIDAERLDMIGLALGQRILALHFDGRSLQSWRHPLV
                     PEQLRGEDVLEDLQLTLWPIEVIEKALPAGWSIADDGRRRTLRLLDQPVLVIDYSAEP
                     RWSGKIELTNLRYQYRLTIQSVSNTP"
     gene            complement(1183..2415)
                     /locus_tag="VE170_14390"
     CDS             complement(1183..2415)
                     /locus_tag="VE170_14390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ16682.1"
                     /translation="MVVEVLIIGAGPAGAVAAALLCRQGLGVLVLEREQFPRFSIGES
                     LLPQSMEYLEQAGMLDAVQRAGFQIKNGAAFGRGEQYSEFDFSDKFSAGWSYTYEVPR
                     ARFDHLLALEAERQGAEIRFRHEVIAVDLSGARPRVTIKDDQNETFVVEAGFILDASG
                     FGRVLPRLLGLERPSDFPVRGALFTHVEDRIVSIPPKTFDRNKIRINVQPDNRDLWYW
                     VIPFSDGRCSIGVVGGLEALARYQGTELERLRGLIAADGATDRLLAQARWDTPVRQLV
                     GYACNVSSLSGPGYALLGNAGEFLDPIFSSGVTIAFKSASLAAALVPRQLAGGLVDWE
                     QEFAIPLKQGVDTFRAFVESWYRGGLQRIIFDPVQRADIRRMIAAILAGYAWDRSNPY
                     VSQTRRRLAALEELCYQG"
     gene            complement(2679..>4244)
                     /locus_tag="VE170_14395"
     CDS             complement(2679..>4244)
                     /locus_tag="VE170_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYJ16683.1"
                     /translation="IWMTFHSLKPIILVTLSMAIGCLGALSISSWLLVERIHLITLVF
                     GASLIGVAQDYGIYFFCQRASAANDSLDSWRLLRRILPAMFLTMVTTLIGYLGLVLTP
                     FPGLRQMALFSCTGLIFAWLTVVCWFPAWVRPTTVGLNRWAGRYGNTMTAWPRLASDR
                     RTLYVALTFAALLLFGGLGLSVQDDIRALQKPPQNLLDDQVKLGKILDVATPVQFYLL
                     RAASAEGLLQREEALKQRLEPLVQQNRISGYQAISDWVPSAHLQASRRQLIERSLLND
                     DGALASIATKLGEAPSWLSAARARLLATGRPITVDDFIKSPASQPWRHLWLGQVGDQH
                     ASIVALRGVTRSSLPALGQAAAGLAGVVWIDQISEISALLGAYRYYMGWVLLVSYVAV
                     YALLYPRYRQASWRVLAPTAVATLLTLALLGIAGQGLQLFHLLALMLLLGIGVDYGII
                     LQEPDSQEDGTAWVAVGISALNTLLSFGLLGLSQTPALRAFGLTMAIGIGTVALIVPC
                     FRQANGAAQATRR"
BASE COUNT          982 a         1331 c         1203 g          728 t
ORIGIN      
        1 cggctgagtg gattacaacg cgtcatgctg accgattcaa gggcggcgaa accggccacc
       61 gtgaaacggc acaaagtgtc gacgcccccg gccagcaccg cgtcgcaaac gttcatgcca
      121 atcagccgcg ccgcgctggc cagcgccttg gcgctcgacg cgcaggcgct ggagtggaca
      181 taggcgggtc cgcaaatgcc cagctccgtc gccaacaccg ccgccggaga aaccaactcc
      241 tgctggccat agtgaaatcc ttggggcaat gctcctgtcg acagataatc acgcaacgcc
      301 gcttccgctt cgccgatgcc ggaagtgctg gtgccgatga cgactccgac ccgtgcgccg
      361 ccgaagcgtt caatcgcact ttgcacggca gggcggattt gctgcaatgc cgctaaaacg
      421 atctggttat tgcggctacg atcaggcaag ggccatgcat ccatcggcgg cagagtaaaa
      481 tcagcatcga tacggcccac cggtaacaag cgcccgggtg aatagacatc ggacaacgcc
      541 acaccgctct gcaccaattc caacagccgt tgcttggttg cgtcgacgct attacccaac
      601 gggctgatca tgcccagctg gttgagatag accctgctca tggagtgttc gaaaccgact
      661 gtatcgtcag gcgatactga tagcgcaaat tggtcaattc gatcttgccg ctccagcgcg
      721 gctcagcgct gtaatcgatc accaacaccg gttgatcgag cagccttaaa gtccgccgac
      781 ggccatcgtc ggcgatgctc cagccggccg gcaaggcttt ctcaataacc tcgatcggcc
      841 acaacgtcag ttgcaaatct tctaagacat cctcaccgcg caactgctct ggcaccagcg
      901 gatggcgcca agactggagc gagcgaccat cgaagtgcag cgcgagtatc cgttgaccga
      961 gcgcgagacc gatcatgtcg agccgctccg cgtcaatttc cagggcggca tcgagttcat
     1021 cgacgcgccc ttgtcgctcg atgcgcagat gctgctgcaa actcagcgaa actcccagcg
     1081 ttgccggtgc gagtttaagg cccagccgcg ccggcagtgc gggggcgtcc gcgcagccga
     1141 acgaatgaac cgcgagtgcc gcgagcagcg cccctccccg gctcaaccct ggtagcatag
     1201 ttcctccaac gccgccaagc gccgccgagt ttggctaacg tagggattac ttcggtccca
     1261 agcgtaaccc gccaggatcg ccgcgatcat gcggcgaatg tcggcacgct gcaccggatc
     1321 gaaaataatt cgctgcaagc cgccgcgata ccaggactcg acgaaggcgc gaaaggtgtc
     1381 aacaccttgc ttaaggggta tggcaaattc ctgctcccag tcgaccaacc caccggcgag
     1441 ctggcgcggc accagagccg ccgccaggct ggctgatttg aaagcgatgg tcacaccgga
     1501 agaaaaaatc ggatcgagaa actcgccggc attgccgagc agcgcataac cggggccgga
     1561 caacgaactg acattgcagg cgtacccgac caactgccgc accggcgtat cccagcgtgc
     1621 ctgggcgagc aatcggtcgg tcgcaccatc ggccgcgatc aacccgcgca agcgctccag
     1681 ttccgtaccc tgatagcgcg ccagcgcttc gagcccgccg acgacgccaa tagaacaacg
     1741 gccgtcgcta aacgggatca cccagtacca caggtcgcgg ttatccggct gcacgttgat
     1801 gcgaattttg tttcggtcga aggtcttggg cggaatactg acaatccgat cttcgacatg
     1861 agtgaacagc gcaccgcgca ccggaaagtc cgacggcctt tccaacccta gcagacgggg
     1921 cagaacgcgg ccgaatccgc tggcgtcgag gatgaagccg gcttcaacga cgaacgtctc
     1981 attttgatcg tccttgatcg ttacgcgcgg gcgcgcgccg ctcagatcca cggcgattac
     2041 ctcatggcga aagcgaattt ccgcgccttg gcgctcggct tcgagagcca gtaaatgatc
     2101 gaagcgcgcc cgcggtactt cataggtgta actccaaccg gcggaaaatt tatcgctaaa
     2161 gtcgaattcg ctgtactgct cgccgcggcc gaaggcggcg ccgtttttaa tttgaaagcc
     2221 ggcgcgctgc accgcgtcga gcatgccggc ctgctcgaga tattccatac tttgcggcaa
     2281 cagactctcg ccgatgctaa agcgcgggaa ctgttcacgc tccagcacca atacgcccag
     2341 tccttggcgg cacagcaggg ccgccgccac tgcaccggcc ggcccggcgc caatgattaa
     2401 tacctctacg accatgctgg ttgacaaggt gctcctagta gaaagagatc agagagtcag
     2461 ccgaaaaaaa acgtaaagtt caccaaaaaa ataattcgga tttaaatatg caaggggcgg
     2521 gattaaacac ccgccctctt cggtttgcat ccgggctgcg ttgtgctgga agttttgccc
     2581 acgccgagta aatttttagc gataatagca cgaagggcgc gaagttgtag cgcttagtcg
     2641 cactttccct ttgccaccga accctggcag caaatatttt accggcgagt tgcttgcgcc
     2701 gccccatttg cctggcgaaa acacggtacg atcagcgcca ccgtgccgat accgattgcc
     2761 atcgtcaacc cgaaggcgcg taatgccggc gtctggctca agccgagcaa cccgaaggac
     2821 aacagcgtgt ttaacgccga gataccgaca gcaacccacg cggtgccatc ctcttgactg
     2881 tccggctctt gcaaaataat cccgtaatcg acgccgatgc cgagcaacag catgagcgcc
     2941 aacagatgaa acaactgcag cccttggccg gcgatgccca ggagcgccag cgtcagcagc
     3001 gtcgccaccg cagtgggcgc caagacccgc cagctggctt ggcgatagcg cggataaagc
     3061 aacgcataga cggcaacgta tgagactagc agcacccagc ccatgtagta gcgataggcg
     3121 ccgagcagcg cggaaatctc gctaatttga tcgatccaaa cgacgcctgc gagtccagct
     3181 gcggcttggc caagcgcggg caagctgctc ctggtcacgc cgcgaagggc aacgatgctg
     3241 gcgtgttgat cacccacttg gcccaaccaa agatggcgcc agggttggct cgccggcgat
     3301 ttgataaaat catcgacggt gatcggccgg cccgtagcca gcaagcgcgc gcgcgcggcg
     3361 ctcaaccagg acggagcttc accgagctta gtcgcgatgc tcgccaaagc accgtcgtcg
     3421 tttaacagcg atcgctcgat cagttgtcgg cgcgacgcct gcaaatgcgc tgacggcacc
     3481 cagtcggaaa tcgcctgata gccgctaatc cggttttgtt gaactaacgg ttcgagccgc
     3541 tgttttagcg cctcctcccg ttgcaagagc ccttcggcgc tggcggcgcg caacaaatag
     3601 aattgcaccg gcgtcgccac gtcgagaatt ttaccgagct tcacctgatc gtcgagtaag
     3661 ttctgcggcg gtttttgcag tgcgcgaatg tcatcttgca cgctcagacc caagccgccg
     3721 aagagcagca aggcggcaaa ggttagagct acgtaaagcg tccggcgatc ggacgccaag
     3781 cgcggccaag cagtcatggt gttaccatag cggcccgccc aacggttcag ccccaccgta
     3841 gtcgggcgca cccaagcggg aaaccaacaa accaccgtga gccaggcgaa aatcagtccg
     3901 gtacaggaaa acagcgccat ctgacggagt ccggggaacg gtgtcaggac caaaccgaga
     3961 tagccgatca gcgtcgtcac catggtcaaa aacattgccg gcaaaatccg ccgcagtaac
     4021 cgccaggaat cgagcgagtc atttgccgcg ctcgcccgct ggcagaaaaa ataaataccg
     4081 tagtcctgcg cgacgccgat caagctggcg ccgaatacca gcgtgatcag atgaatgcgt
     4141 tcgaccagga gccatgaaga aatcgacaac gcgcccaaac agccgatggc catggacagg
     4201 gtgactagaa taatcggctt gagcgaatga aacgtcatcc agat
//
LOCUS       DASQKS010000563         3271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_740516, whole genome shotgun sequence.
ACCESSION   DASQKS010000563 DASQKS010000000
VERSION     DASQKS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740516"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VE170_14400"
     CDS             <1..1365
                     /locus_tag="VE170_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ16684.1"
                     /translation="SASTPHPLGPTRQISTFRSTLTRDLTQPRLRLEWESGNAARNQT
                     LRFVEVIDGDAGSLREGATDTAKVSRLHPGRLATRLREEKRAPAKILLTAANQNSLRR
                     RADAEVDGQRNYVLSFIDNNDEFHLYIDAKSHFPTLVDILEDDPLEGDSSYVLRYSDW
                     RKSGALMLPFILRTELNGKLLQEEQIKSIQHNVTFAADPFAISPEVRNEKPDAKPIAS
                     QWILRRVAGNVSYLDFGRPPAVEWHRLADGVHKIQGVGHATIVVEMRDHLIAVEAPLY
                     DQRTAAVVQSIKDKFPDKPIRYVIPTHHHLDHAGGIRAFMATGATVVVPFAAREFYRR
                     VASAPHTRRPDSLTKQPAGVVIEAFDGGSRILGDGQRRVEVHPLPLPHAEDMVVIYLP
                     AEKIIIEADHISPRKGEVRPAATVREFVAALDRLNLDVVTIVGIHGDSATLQAARTAA
                     QATK"
     gene            1380..1955
                     /locus_tag="VE170_14405"
     CDS             1380..1955
                     /locus_tag="VE170_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYJ16685.1"
                     /translation="MIKRSAPIVALWIGLIAGSWTNGRAQEKPEPAPADRSARPLIRV
                     PTALPLDEARVIVEAAKALVKAENGRAAIAVVDFNGNLVSLDSMDGTSGFWVKFAIGK
                     ALGAVTLQQSTAESGDQIKTNPQRFHSALSLMEGQIILIPGGFPLIVDNVIVGAVGSA
                     GHRGDGDVRAVKAGMAAWEKFRQGKKSESMR"
     gene            1993..2430
                     /locus_tag="VE170_14410"
     CDS             1993..2430
                     /locus_tag="VE170_14410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ16686.1"
                     /translation="MAAEFIDIMQEPELDIGHSKKVLFTSDQFHTWVHGDYPGTKGPM
                     HKHTADQQFYCVKGQCTFNFPDAPSRALNPGMLVTIPAGQLYQLDNTGDEYMILLGAR
                     AEPAGNPRFSMTDREVGHGDFAKNNLDKIKAPEEKKARARQES"
     gene            2615..>3271
                     /locus_tag="VE170_14415"
     CDS             2615..>3271
                     /locus_tag="VE170_14415"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16687.1"
                     /translation="MASRNRTLNNLNKLKWRAPILSLAVILCAAGWSSSLFAADRVKL
                     ALPAKSMGYLPLFVASHRGFLKDENIEIETPMMLPQLAHNALLSGEVDYHGVADSALR
                     LAAKGAPIKTIFFGASRPNYFLMAKPQVKSIGELRGKYIGIVRFGDTIELATKLAFNR
                     EGMDAQRDAMLIMIGLPGTRVAALSAGSVDATVVVPPDNVVLRQKGFRELLFLGDAIE
                     F"
BASE COUNT          724 a          997 c          916 g          634 t
ORIGIN      
        1 tcagcctcga caccacatcc gctcggaccg acccggcaga ttagtacctt tcgctccact
       61 ctgacccggg acctgaccca accgcgcctt cggctcgaat gggaaagcgg caacgccgcg
      121 cgcaatcaga cgctccgctt cgtcgaggtc atcgacgggg atgccgggtc actgcgcgaa
      181 ggcgccaccg atacggcgaa agtcagccgt ttacaccccg gccgactggc cacccgcctg
      241 cgtgaagaaa aacgcgcgcc ggcaaaaata ttgctcacag ccgccaacca aaactcactg
      301 cgccggcgag ccgacgcaga ggtagatggc caacgcaact atgttttgag tttcatcgac
      361 aataacgacg agtttcacct ttacatcgat gccaagagcc actttccgac cctggtcgac
      421 atactcgaag acgaccccct cgaaggtgac agcagctatg ttttgcgtta tagcgattgg
      481 cgtaagagcg gcgcgttaat gctgccgttc attttgcgca ccgagctcaa tggcaagcta
      541 ttgcaagagg aacagatcaa atcgatccag cataatgtta cctttgccgc cgacccgttt
      601 gccatctcgc cggaagttcg caatgaaaaa cccgacgcca aaccgatcgc gtcgcaatgg
      661 atactgcgcc gcgtcgccgg caacgttagc tacttggatt ttggccggcc accggcggtt
      721 gaatggcatc gcctggccga tggcgttcat aaaatccaag gcgtcggcca tgctaccatc
      781 gtggtcgaga tgcgcgatca tttgatcgcc gtcgaagcac cgctgtacga ccaacgcacc
      841 gcggccgtgg tgcaatctat caaggataaa tttcccgaca agccgattcg ctacgtgatc
      901 ccgacccatc atcatctcga ccatgccggc ggcatccgcg ccttcatggc caccggcgcc
      961 accgtcgtcg tccccttcgc cgccagggag ttttaccgtc gcgtcgccag cgcaccgcat
     1021 acgcgcagac cggacagctt gacgaaacag cccgccggcg tggtcatcga agccttcgac
     1081 ggcggttcgc gcatcctcgg cgacggccag cgccgcgtcg aggttcatcc cctgcccttg
     1141 ccccacgccg aagatatggt cgtcatctat ttaccggcgg agaaaattat tatcgaggcc
     1201 gatcacatca gcccgcgcaa aggcgaagtg cgaccggcgg cgacggttag agaattcgtt
     1261 gcggcgctcg acaggttgaa tctcgatgtc gtgaccatcg tcggcatcca cggagatagc
     1321 gccacgttgc aagccgcgcg gacggcggcc caggcgacta aatgaatatg gaggacagcg
     1381 tgataaaacg gtcagctccg atcgtcgccc tttggatcgg cttgatcgcc ggctcatgga
     1441 caaatggccg cgcccaagaa aaacccgagc ccgcgccagc cgatcgctcg gcgcggccgc
     1501 tgatccgtgt gcccaccgca ctgccgttgg acgaagcgag ggtcatcgtc gaagcggcaa
     1561 aagcactcgt caaagctgaa aacggccgcg ccgccatcgc cgtggttgac ttcaacggca
     1621 atctggtttc actcgactcc atggacggca cgtccggctt ctgggtcaag ttcgccattg
     1681 gtaaagcgct cggcgcggtc acactgcaac agtcgacggc cgaatccggt gatcagataa
     1741 agaccaaccc gcaacgtttc cacagcgccc tcagcctgat ggaaggacag atcatattaa
     1801 tccccggcgg ctttccgtta atcgtcgaca acgtcatcgt cggcgccgtc ggcagcgccg
     1861 gccaccgcgg cgatggcgat gtccgcgcgg tcaaggccgg catggcggcg tgggagaagt
     1921 ttcgccaagg caaaaagtcc gaatcaatgc gttaagaaaa agcgtcaaaa aaccggcaag
     1981 gagaaaaaaa atatggctgc tgaattcatc gatataatgc aagaaccgga actggatatc
     2041 ggtcacagca agaaagtgct gtttacctcg gatcaatttc acacctgggt acacggtgat
     2101 tatccaggga ccaaggggcc gatgcacaag cacaccgccg accagcagtt ttactgcgtc
     2161 aaaggccagt gtacgtttaa ctttcccgat gcgccgtcgc gtgcgctcaa tccaggcatg
     2221 ctggtgacga tacccgccgg ccagctttat cagctcgata acaccggcga tgaatacatg
     2281 atcctcctcg gtgcccgcgc cgagccggcg ggcaatccgc gctttagcat gactgaccgc
     2341 gaagtcggtc acggtgactt cgccaagaac aatttagata agatcaaagc cccggaggag
     2401 aagaaagccc gggcgcggca agaatcctaa agccgctttc ggcaatattt tgtttatcgc
     2461 cgattcaggt agggcgggag tttgtcatga cggcgcgttg acgcggctta ggaataactc
     2521 tccaggtttt tcccgggaag cactgcgcct gcgccgggct gggcaatagc agtcgacttc
     2581 gagccaatgc atgtgtaact cggtttaata ggtaatggcg tcgaggaatc ggaccttgaa
     2641 taacttgaat aaattgaagt ggcgagcgcc aatcctcagc ttggctgtca tcctatgcgc
     2701 cgccggctgg agttcttcac tcttcgccgc tgaccgggtc aaactcgcgc tgccggcgaa
     2761 gtcgatgggt tatctcccgc tctttgtcgc cagccatcgc ggtttcctca aagacgagaa
     2821 tatcgaaatc gagacgccga tgatgctgcc gcaactggcg cacaatgcgc tgctctccgg
     2881 cgaagtcgac tatcacggcg tcgccgactc ggcgttgcgc ctggccgcca aaggcgcgcc
     2941 gatcaagacg attttttttg gcgcgtcccg gcccaattat tttttgatgg ccaagccgca
     3001 ggtgaaatct atcggcgaat tgcgcggcaa atatatcggc atcgtccgct tcggcgacac
     3061 catcgaattg gcgaccaagc tggcttttaa ccgcgaaggc atggacgccc agcgtgacgc
     3121 tatgctgatc atgatcggtc tgcccggcac tcgggttgcc gcgctcagcg ccggttcggt
     3181 cgatgccacc gtcgtcgtac cgccggataa cgtcgtgctg cggcaaaaag gttttcgcga
     3241 gctgctattt ttgggcgatg ccatcgaatt t
//
LOCUS       DASQKS010000564         6733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3267391, whole genome shotgun sequence.
ACCESSION   DASQKS010000564 DASQKS010000000
VERSION     DASQKS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267391"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VE170_14420"
     CDS             complement(<1..1342)
                     /locus_tag="VE170_14420"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HYJ16688.1"
                     /translation="MKLLQTLRRRKRRFFKSLLLGVGVSILVSISSYLGYLDSIEAKA
                     LDLLLRLRGQQRSPGIVLIKIDDQAFEKLGEKQPLPRNYIASLVDIAARGGAKVVGID
                     IELKVATDPAEDEKLIKAIAVAEANGVTKIVPVYVIRPGKEDAYGATYRHAPFFDPGL
                     KVGGGFANAPMDDDGIVRQVPLAVRGEDGTILPSLALAVLARHAGYDSASLNRQLRGG
                     ATVRLNLPEWHKLNGKLLSQSTSFEFHLDESWKINFAGGQGSFASLPSEPLAQLARAN
                     IPLATDNPFRGKIVLIGASFQDSRDFFATPNGLMSGLEIHANIIHTLLTRSHIRPANR
                     ILAMLLLLIFAVAASVLTTLLRPATVMVVSLVAIPVVLLPLSYAALTQLGLWVDFVTP
                     LLVTSWGALATEYRDNRQVRKFLANMLDGKSPSRSSIRTKICKERAVRSRYFLPM"
     gene            1626..4625
                     /locus_tag="VE170_14425"
     CDS             1626..4625
                     /locus_tag="VE170_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYJ16689.1"
                     /translation="MKPSNFFLAIQILALLLSNNSFAASVTVPRVPEELKKILDTPPP
                     DPKEHSKAELTKIYMERGRAASTLGDLDRELKELDAGIQAVGAKDPNAYEFYNRRAPL
                     DLDRGDYVSARAARELALEVAGSAGRQFFQLNYLSSISAVLRDRESAKTYLSRADSLL
                     ASVRRGNPDWGRFGNFWQAALSESKGNYNLSFGYLIEAQSHYRTCTTAIRSYMGKNPN
                     PADNSYYYLPQCLGRSVELAARLGYLREAGAYVNDVRETAKAYAQAHQRVLFETRMTR
                     PIARVYLEQGLSTEAKELIDSTINRSERFQQGEASLQVGAARLFAAQLEMIQGNWLKA
                     EEHFRARRDGLRANKAQAEELDTENVPDWGYALLRLGKTQEAMNVLNADLTASQKRFD
                     DESLYLWDSRAFHALGIGAAGDKEAAVKTLAVTIPKLLELGRGQGSTESGFLTSARTG
                     WILDGYIALLADLHRAKTRSAGIEPHVEAFRIADLARASRVEKALSAAIVRASINDPQ
                     LAAVLRRAQDLDYQVKAISETLTALQQGVKTDKPDPNREKAIEKTRAELARVREENDQ
                     AQTELKRKLPDYSEMLNAKPLTISDAQKLLKPQEALISLYSTQQHTLVWAMAGQGQPV
                     FQVVDLPSAKVAELVTKLRKALDPTEADISQVPTFDFATALELYQKLLAPVEAGWKNA
                     KELIVVPHGALSELPFSVLVTSAYQPNKSGIPFSDHGGASWLIKNAAISYLPSLSALA
                     SLRRAVAPSAANSFIGFGDPVFGGGAKSAAPVAARGMVRRNAKAAAAPSSSTSNLELL
                     QALPDTADEVRDIAKILRADESRDVHLGRRASEQSVKNVDLTNYRVVMFATHGLVPGE
                     LPDLAQPALALSNPAITGEKEDGLLTLAEILGLKLRADWVVLSACNTASADGQASEAV
                     SGLGRAFFFAGAKALLVSHWPVETVSAKLLTTELFKRQSSDAKTSRAQAVREASLAVM
                     QQQSAGKTYSYAHPMFWAPFVVFGDGG"
     gene            4722..6533
                     /locus_tag="VE170_14430"
     CDS             4722..6533
                     /locus_tag="VE170_14430"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HYJ16690.1"
                     /translation="MFSPEQLADLLAMPFFAGLRPATLHQLQSDLEPVHLPGGALLFA
                     AGAVGDSMYVVLSGRLRVSIERDDGSPETVRELARGDTVGELALLTGEPRSAAVNAIR
                     DTRLAKISRATFESAVKSDPKLLGLIAVQLARRERQGRDATLLGRNLRTIAVVPVDTQ
                     VSLPEFTAALTYAMETVGATACLRPEVLPALCELHGAPAAASNNVEHLGALLNHLETN
                     HRFVLYQADPDLSEWTELCLRQADLILLVGAADCPPEVSRLEIMRNYFRQNHIATTME
                     LVLLHHPQFITTVRAARWRDALDFAAHHHVVPSRLGDIARLVRTFTGTAVGLVLSGGG
                     ARGFAHIGVIRALTERQIPIDFIGGTSMGAVIAAQHALGWDWSTMAQVNRAEWARCHP
                     QKNYTLPLIAINSGNRMDRMLTRMLGDATIDDLRTDFFCVSTNLTRAQAKIYRQGSLW
                     KAVRASVSIPGIGPPTIENGEIFVDGGLVDNLPVAVMKSFCHGKVFAVDVSEQAEFKS
                     TLEQSYGVSGWKCLWQRLNPFVITPDLPNILNTLYRTTTVAGVRAIESAKAQADLCFE
                     PPVNHFGVFDWRSVDAIIEAGYRYALDKLDASYSEKT"
     gene            complement(6552..6733)
                     /locus_tag="VE170_14435"
                     /pseudo
     CDS             complement(6552..6733)
                     /locus_tag="VE170_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
BASE COUNT         1495 a         2007 c         1890 g         1341 t
ORIGIN      
        1 acatcggtaa aaaataccga gacctcacag ctctttcctt gcaaatcttc gtcctgatgg
       61 acgatctgct cggcgacttc ccatccaaca tattcgccaa gaatttacgg acttgtcggt
      121 tatcgcggta ctccgtcgcc agcgcacccc agctagtgac caacagcggc gtgacgaaat
      181 ccacccacaa ccccaactgc gtcaacgcgg catagctcaa tggcaacagc actacgggaa
      241 tggcgaccag tgatacaacc atcacggttg caggccgtag cagcgtggtc aacacgctcg
      301 cagcgacagc gaagatcaat aatagtaaca tcgctaagat ccggttggct ggccggatgt
      361 gcgaccgggt caaaagcgtg tggataatat tggcgtgaat ttccagaccg ctcattagac
      421 cgttcggcgt cgcgaaaaag tcacggctgt cctgaaaact tgcgccgatc aaaacgatct
      481 tgccacggaa cggattatca gtggccaacg gaatgttggc tcgcgccagt tgcgccaacg
      541 gctcactcgg cagggatgca aaactgcctt ggccgccggc gaaattgatt ttccagcttt
      601 cgtcgaggtg aaattcaaaa gacgttgact gcgacagaag cttaccgttg agcttgtgcc
      661 actcgggcag attcaaacga acggtggcac cgccgcgcaa ttgacggttt aaacttgcgc
      721 tgtcgtagcc cgcgtggcgc gccagaactg cgagcgccag ggatggcaga atcgttccgt
      781 cttcgccacg caccgccagc ggtacttggc ggacaatccc atcgtcatcc atcggcgcgt
      841 tggcaaaccc gccgccaact ttgagccccg gatcgaaaaa cggcgcatgg cggtaagtag
      901 cgccgtacgc atcctctttc ccgggccgaa tcacataaac cggcacgatc ttggtcacac
      961 cgttggcctc ggcaacggcg atcgctttaa tcaatttttc gtcttccgcc ggatccgtag
     1021 ccacttttaa ttcgatatcg atgccgacca ccttagcccc gccgcgggcg gcgatatcga
     1081 cgagactcgc gatatagttc cggggcagcg gctgcttctc gccaagtttt tcgaacgcct
     1141 ggtcgtcgat cttgatgagt acgatccccg gcgagcgctg ctgaccccgc agtcgcagaa
     1201 gcaggtccaa ggctttggct tcgatcgaat ccagatagcc cagataagaa gagatggaaa
     1261 ccaagatgct aacgcccaca cccaacagaa gagacttgaa gaatcgccgc ttgcgccgcc
     1321 gcaatgtttg gagcagtttc atctgccgac catggctccc ccatgaggcc gaatgcagcc
     1381 cagcaatcgc ggtaagcaga ccgcgccgcc agtcgaaatt aggattttca ccacacggaa
     1441 gtcgcagcca ctagccataa tatgcaaaaa caaaacgaca gatgggcgcc agagtagtca
     1501 aaaaccgtgc gaaaaggaag ttgagatggg attgacggac tagcgtcgtt ctcttaactt
     1561 agcgtgaaca acgtaacgtc ccgtgcgaaa agactatcgg cagctgctag tcttcggagg
     1621 atttagtgaa gccatcgaat ttttttctcg ccatacagat tctggcatta ctcctgagca
     1681 acaacagttt tgccgccagt gtaaccgtcc cgagggtgcc agaagagtta aagaagattc
     1741 tcgacacgcc accgccggac cccaaggaac actccaaagc ggaattaacg aaaatctata
     1801 tggagcgcgg ccgagcggcg agcacgcttg gcgatttgga ccgggaattg aaagaactcg
     1861 atgccgggat tcaagccgtc ggcgccaaag atccgaacgc ctatgaattc tacaatcgcc
     1921 gcgcgccact cgatctagac cggggcgact atgtcagtgc ccgcgccgcg cgcgagttgg
     1981 cgctagaggt cgcgggaagc gccggacgac aattctttca actcaattat ctgagctcga
     2041 tcagcgccgt actgcgcgac cgcgagagcg ccaaaaccta tttatcgcgc gccgacagcc
     2101 tgcttgcctc ggtgcgccgc ggcaatccag actgggggcg tttcggcaat ttctggcaag
     2161 cggcgctaag tgagtccaag ggcaactata acctttcttt cggctacctc atcgaagctc
     2221 agagccatta ccgcacctgc accacggcga tccgcagtta catgggcaaa aacccgaatc
     2281 cggcggacaa tagctactac tatctgcccc agtgtctggg tcgctccgtt gagttggcgg
     2341 cgcggctcgg atacctgcgc gaggcgggcg cctacgtaaa cgatgttcgc gaaaccgcga
     2401 aagcctatgc ccaagcgcat cagcgggtgc tgttcgaaac gcgcatgacg cggcccattg
     2461 cgcgggtata tctcgaacag ggactgtcga ctgaagctaa ggaactgatc gactcgacga
     2521 tcaaccgaag cgagagattt caacagggcg aagcgtctct gcaagtgggc gctgcccgtc
     2581 tcttcgccgc gcaattggaa atgattcaag ggaattggct taaggccgaa gagcactttc
     2641 gtgcccggcg cgacggttta cgcgccaaca aggcgcaggc ggaagaactc gacacggaaa
     2701 acgtgcctga ctggggctac gcgcttctgc gtctcggcaa gacccaggag gcgatgaatg
     2761 tccttaacgc ggatctcacg gccagccaaa aacgattcga cgacgaatcc ctatatttgt
     2821 gggatagccg tgcgtttcat gcgctcggta tcggcgctgc cggcgataag gaagccgcgg
     2881 ttaagacctt ggcagtcacc attcctaaac tcctcgagtt aggacgcggc caaggatcca
     2941 cggagagcgg ttttctcaca tcggctcgga ccggttggat cttggacggc tacatcgcgc
     3001 tcttggccga tctgcaccgc gccaaaaccc gttccgccgg catcgaacct cacgtcgaag
     3061 cgtttcgcat cgccgatttg gctcgggcca gccgcgtcga gaaggcgctc tccgccgcca
     3121 tcgtgcgcgc cagcatcaac gacccacagt tggccgcggt actgcgacgc gcacaggatc
     3181 tcgactacca ggtcaaagcg atctcggaga cgctgaccgc cctgcagcaa ggcgtcaaaa
     3241 cggacaagcc cgatcctaac cgcgagaaag cgatcgagaa aactcgcgcc gaactggcac
     3301 gagtgcgcga ggaaaatgac caagcccaaa ccgagttgaa gcgcaagctg cccgactatt
     3361 cggaaatgct gaatgccaaa ccacttacca ttagtgacgc gcagaagttg ctaaaacccc
     3421 aagaagcgct gatttctctc tactcgacgc aacagcatac gcttgtctgg gcgatggccg
     3481 gccagggaca gccggtattt caagttgtcg atctgccaag cgcgaaggtt gccgagctcg
     3541 tcaccaaatt gcgcaaagct ctcgacccga ccgaagccga catcagccaa gtgcccactt
     3601 tcgatttcgc cacggcgctg gaactctatc aaaaactctt ggcgcctgta gaagcgggat
     3661 ggaaaaacgc caaggaactg atcgtcgtgc cccatggggc actgagtgaa ctgccttttt
     3721 cggtgctagt cacgagcgct tatcagccga ataaaagcgg tattccgttt agcgaccatg
     3781 gcggcgcctc ctggctgatc aaaaatgccg caatttctta tctgccatcg ttgtcagccc
     3841 tggcaagcct gcgccgcgcc gtagcaccgt cggcggcgaa tagttttatc ggcttcggcg
     3901 atccggtgtt tggtggcggc gcgaaatcag ccgcgcccgt cgctgcgcgc ggcatggtgc
     3961 ggcgtaatgc caaagccgct gcggcgccga gttcgtcgac gagcaacttg gaattgctcc
     4021 aagccctgcc tgacactgcc gatgaagtgc gcgacatcgc gaagattctc cgtgccgacg
     4081 agagccgcga tgtccacctg ggcagacgcg ccagcgagca gtcggtaaag aacgtcgacc
     4141 tgacaaacta ccgggttgtc atgttcgcga ctcatggctt ggttcccggt gaattaccgg
     4201 acctcgctca accggcattg gcactgagca acccggctat tactggagag aaagaagacg
     4261 gtctgctcac actcgcagaa atactaggac tcaagctgcg tgccgattgg gttgtcttat
     4321 cggcctgcaa taccgcatcc gccgacggcc aagcttccga agcggtctca ggcctcggcc
     4381 gtgccttttt cttcgccggg gcgaaagctt tgctggtatc ccactggccg gtagaaacgg
     4441 tatcggcgaa actgcttacg acggaattgt tcaaacgcca atctagcgat gcaaagacca
     4501 gccgcgccca ggctgtgcgc gaagcttcgc tcgcggtcat gcagcaacaa tcggctggca
     4561 agacgtacag ctatgctcat ccgatgttct gggcgccctt tgtcgtcttc ggcgatggcg
     4621 gttaagtcag taagtcgtag ttgcttgcaa acctattcag caatcaagta tgtcaagtcc
     4681 gtgctaaaca aaatgtcatc gcgcgttaac cggcgggccc gatgttttca ccagaacaac
     4741 tcgccgatct cttagccatg ccatttttcg ccgggctgcg tccggcgact cttcaccaac
     4801 tgcaaagtga tttggagccg gtccatctac ccggtggggc gctgctcttc gccgctggcg
     4861 ccgtcggaga ctccatgtat gtcgtgcttt ccgggcggct acgcgtctcc atcgaacgcg
     4921 acgacgggag tccggaaacg gtccgcgaac tggcgcgtgg cgataccgtc ggtgaacttg
     4981 ccctattgac cggcgaacct cgctccgcgg cggtgaacgc cattcgcgat acccggctgg
     5041 cgaagatttc ccgggcaact ttcgagagcg cggtcaaatc ggaccccaaa ctgctcggac
     5101 tgatcgcggt gcagcttgcc cgccgagaac ggcaagggcg cgatgcgacg ctgttggggc
     5161 gcaacctgcg gaccatcgcg gtggtgccgg ttgatactca agtgagttta cccgagttta
     5221 ccgcggcatt gacctacgcg atggagacgg ttggcgcgac ggcttgcctg cgccctgaag
     5281 ttttgccggc cctttgcgaa ttgcacggtg cgccggcggc agcaagtaat aacgtcgaac
     5341 acttgggcgc cctactgaat cacctcgaaa ccaatcaccg cttcgttctc tatcaggccg
     5401 acccggacct ttccgaatgg accgaacttt gcctccgcca agccgatttg attctgttgg
     5461 tcggcgccgc ggactgcccg ccggaagttt cgcgcttgga aataatgcgc aattactttc
     5521 ggcaaaatca catcgccacc acgatggaac tggttttgct ccatcacccg cagttcatca
     5581 caaccgtgcg cgccgcccgc tggcgcgatg cgctggattt cgcggcgcat caccatgttg
     5641 tgccgtcgcg gttaggcgac atcgccagac tcgtccgcac tttcaccggc acggcggtgg
     5701 gattggtact cagcggcggc ggcgcccgtg gtttcgctca tatcggcgtt attcgcgctc
     5761 ttaccgaacg acaaattccc atcgatttta tcggcggcac gagcatggga gcggtgatcg
     5821 ccgcgcaaca tgcgctcggc tgggattggt cgaccatggc gcaggtcaac cgcgctgaat
     5881 gggcacgctg ccatccgcag aagaattaca cgctgccgct catcgcgatc aattccggca
     5941 accgcatgga ccggatgctg acaagaatgc tcggcgacgc aacgatcgac gatctgcgca
     6001 ccgacttctt ttgcgtgtcg accaacctga ctcgcgccca agcaaagatt tatcggcaag
     6061 gttcgttatg gaaagccgtc cgtgccagcg tgtcgatccc ggggatcggc ccgccgacca
     6121 tcgagaatgg tgaaatcttc gttgacggcg ggttggtcga taatctgccg gtcgcggtta
     6181 tgaaatcttt ttgccacgga aaagtcttcg ccgtcgacgt cagcgagcaa gcggagttca
     6241 agagcacgct ggagcaaagc tacggcgtgt cgggctggaa gtgtctctgg caacggctca
     6301 atccctttgt cattacaccc gacctgccga atatcttgaa tacgttatat cgtaccacca
     6361 cggtcgccgg tgtgcgcgcc attgagagcg ccaaggcgca agccgatcta tgcttcgagc
     6421 cgccagtcaa ccatttcggt gttttcgatt ggcgtagtgt cgacgccatc atcgaggccg
     6481 gctatcgtta cgccctggac aagcttgacg caagttacag tgaaaaaact tgacggttaa
     6541 tcgatcggcc catagatcga gtcgacaatg gcgtcggtga gctcgcgggt ggcgccatcg
     6601 aaaactatct ggccgtcttt caaacctatc acgcggtcgg cgtactcgcg cgtgagttcg
     6661 agcgtatgca ggctcaccag cgcggtaatg ccgtcttcgc ggcagactct tttgagaata
     6721 tccaagacca cgc
//
LOCUS       DASQKS010000565        12447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1168679, whole genome shotgun sequence.
ACCESSION   DASQKS010000565 DASQKS010000000
VERSION     DASQKS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168679"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..500)
                     /locus_tag="VE170_14440"
     CDS             complement(69..500)
                     /locus_tag="VE170_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16691.1"
                     /translation="MTPRAKSLLALLLSMLIPGLGQIYNREQKKGWAIIACCLVLGIA
                     AYGLSGFNAITAALALLLIWLSAIVDAYKVATSTEQTAEFYYAKSYVITMLLLVGPLA
                     LPLLWQSPNFTNTARWLWTVVVVGAALLFVVTPYLLQSLLV"
     gene            complement(846..1847)
                     /locus_tag="VE170_14445"
     CDS             complement(846..1847)
                     /locus_tag="VE170_14445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16692.1"
                     /translation="MIIDSHTHFTTAPPQLQAYRGQQISNLAKPIRPKLQISDEELAR
                     SMAGQFKRMEECGIDRLLFSPQAGAMGHHFGSPLVSRYWTEACNDLIARVAKTWPDKV
                     SPVCQLPQSPGVSPTEWADELDRCVKDLGFISCNVNPDVAGGKEPLTPSMGSEWWYPL
                     WEKMVALDVPCTVHASASLNPAMHLTNSYYIAHHHSAAVEILKSRVFKDFPKLKIIIP
                     HGGGAIPYQWNRQRGLHVREGLEPFEEAARRVYWDMAIYDKESMELLIKRVGADRVLF
                     ATEMFGAVNAIDPETGRNFEDLVPIFKSVEGLSEQDRYNITEGNARRVFSRLFAEKK"
     gene            2122..2985
                     /locus_tag="VE170_14450"
     CDS             2122..2985
                     /locus_tag="VE170_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYJ16693.1"
                     /translation="MPDIPRLNGVIKALEQGQTAFVAFTPVDIETAIAMAGTKLDGVA
                     FEMEHAPLDFPGLRNALQFMLDRGQIVAGGTLAPAVTPMVRVPPNGIEMNSWIAKQVL
                     DIGVMGIIFPHVSSVEEAANAVGACRYPRLKSAPRYYPPGIRGDAPARAARYWGLPQQ
                     EYYKRADVWPLAPDGEVLVVIQCEEMRAIENLPKILKEVPGIGVVLIGEGDLSQELGH
                     PRDYDHPVVADAINNILKICKEHNVPCGHPHPDGKNIERLVKDGYRFIMPSSPRSFGV
                     LDQGRKLTGRS"
     gene            complement(3153..4277)
                     /locus_tag="VE170_14455"
     CDS             complement(3153..4277)
                     /locus_tag="VE170_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16694.1"
                     /translation="MRLLNCQCRFASLLIAWVFLCSASRAISAEVSFRSPQWDATVEA
                     AKKEGKVNIYLYRYGKVLDVFRSDYPEIRPYLLTGTGAQITTKILTERRAGRFLADVV
                     GLGSSNYRILHQQAKMLEPIQPALMLPEVLDTTRWYGGKHRYLDAENKYVFAYLANAS
                     SGQLYYNSAQVSAKEFTSYYDLFKPKWKGKIVALDPRPRTEIGTTMQFFYYHPELGPG
                     FIRQLFGAKDITFSRDSRQMIDWLGQGRFSICFGCSGGLKAKHQGLPIEIFDTSLWKE
                     GASFSVGGGTMSTPSQGAHPNAAKVFINWYLSRRGQMALQKLGDPDEPPNSARIDIPK
                     DDIQMHNRLIDGRKYFDGTRPEFEDIDSAFKLAVQAMEGR"
     gene            4520..5272
                     /locus_tag="VE170_14460"
     CDS             4520..5272
                     /locus_tag="VE170_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16695.1"
                     /translation="MLKTSCYAALLLLLTSVALAQNPGKIPREVIDQAAANGTALVLV
                     GLNVPWQTEARLSDAELRAQRSAIASIQSDLLNQLEGRKYKLLRRYQEVPGIALEVGA
                     DALAELARLPIVTNVLLDRPAGTAMPPANRNAAARAAGADLASGEKVPSILFTQAARD
                     GTVLVLAGLRTPWQHEEGMSEELWALQRQAIHSTQSYLLAELAGTRYKVMRLYGKIPG
                     IALRVGVDALIVLEKSPAITNVVLDRPAQVRR"
     gene            5341..6951
                     /locus_tag="VE170_14465"
     CDS             5341..6951
                     /locus_tag="VE170_14465"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYJ16696.1"
                     /translation="MSKFAEFCVRYFKQHFELHPTDAIYYGVEGYDHLLNDYSDKSYK
                     AEKIFVKESLRKLGEISRAELTADESIDYALLEGRLSIQSYEQAKEDYRLKQPDIYSP
                     TEAIYILTVRATNDLAANLLSRLNRTPALIQQGITNLNRKDANPPKIWTENAIEGAKG
                     GITFLDSLPEHPKVQAAAIEANILAAAIEKAKSAIADFAIFLERHLLPRSNASYAVGE
                     EHFNLLLRKKHFLVHDAHSLLALGESLFAETKLELEALADELAPGKGVETAARGIQEN
                     HPSSDAILSVYDQAMRAAREFVREKKLVSFPAREELHVVHTPVFRRHEIPFAAYLSPS
                     PQDPDQVGYYYVTPVSSDDLLREHNYVGLENTSVHESYPGHHLQFSIANCTPTAATLP
                     RLMNESSVFYEGWALYCEQLMQEQGFLKSKEHRFVMLKDRLWRALRIIIDVKTQTGKL
                     SYDEAANLMVRELHFPREQACGDLNWYSQSPSVPMGYALGWSIINRLRDQEQKRLGGK
                     FNLCEFHDKLLSSGSISLPLVEKRHFMN"
     gene            7051..7926
                     /locus_tag="VE170_14470"
     CDS             7051..7926
                     /locus_tag="VE170_14470"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HYJ16697.1"
                     /translation="MKNKEKPRGPLLGAHMSIAGGVGNAFLEGRKVACEAIQIFTKSS
                     RQWASKPLSEDEIAQFHANRKETGIAAVVAHDSYLLNLGSPDPALRNRSIIAFIDEME
                     RCEVLGVSNLIAHPGAHIGAGELEGIKTIAKSLDEVHTACPGFAVKVTLEITAGQGSN
                     LGYRFEQIGDMMDATKENDRLRVCFDTEHAFAAGYDIRTKEGYERTFAEFDQTIGIEL
                     LAAFHLNDSKKEFHSRIDRHEHIGQGFIGVEAFRLLMNDRRFWGLPMCLETPKGPDLK
                     EDRENLTLLRSLIAV"
     gene            complement(7979..8707)
                     /locus_tag="VE170_14475"
     CDS             complement(7979..8707)
                     /locus_tag="VE170_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HYJ16698.1"
                     /translation="MSLDTIQQKKRVIESRARIREFVFGIQDGLISTVGLLAGIQSAT
                     ESNLVVIVTGFTAMFSGAISMATGSYLSSGAQKDIFDKELVDAEMLAEREPYIAAEGL
                     LKALGEEGLSKEQSYRMVKVLLAERNVFMRTFQEKVFGLGSAEINQPFKAALVMGLSF
                     IVGAIIPIVPFIATEGVVALSLSVFLSAATLFLVGAFKGYLAGQSVLISGAKFFGVAV
                     SAGGLGYLIGLAVQWFFPGISIPA"
     gene            complement(8781..9305)
                     /locus_tag="VE170_14480"
     CDS             complement(8781..9305)
                     /locus_tag="VE170_14480"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ16699.1"
                     /translation="MRIGEPAPDFKVKGILRDQVGEYSLRASNSKWLVLFFYPADFTF
                     ICPTEVTGFSKAVKDFAAEDAMVWGVSVDSIDSHRSWSAELGGLDCPLLSDETTTLSR
                     AYGVLDENAGVALRATFIINPAGIISYQVVSHVNVGRSVEETLRVLKALRTERLCPSD
                     WKPGEATGDLALKY"
     gene            complement(9392..10075)
                     /locus_tag="VE170_14485"
     CDS             complement(9392..10075)
                     /locus_tag="VE170_14485"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ16700.1"
                     /translation="MSSRAEPATRHYDEKLQLILKTAAKIFAEKGFHHTSVRDISRAT
                     KMSLSGLYYYFTTKEELLYLIQERCFVTLLEHWEQIDSRGLDVRGRIRAFAENHLGFF
                     LHNMPEMKVMAHEDESLTGEFDEKILVFKRRYVKVIMDLLGELQETSGAKAMDLRLAT
                     FSLFGMMNWIYTWYQPKRDLPISQLIEQMLRIYFFGLLKAGSEEESWFTAAPPANAEN
                     GFSLWQHKP"
     gene            complement(10544..11722)
                     /locus_tag="VE170_14490"
     CDS             complement(10544..11722)
                     /locus_tag="VE170_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ16701.1"
                     /translation="MIPQLSIQERDRRYQLVRAEMAQRGIDVLLLPANTGRWEQLQGD
                     SRYLTNIGGFATEMFTVFPAQGDVTAYVFNRAGWWQKAQNWVADVRDGRNRWSQNAIE
                     RIKELGLDKGTIGVSGLAGLVRAPEGIIPYTSVKAIQEAFPHAKLVNATEMMQEIRAI
                     KSSEEVSFLERSAAIIEKAIDAMKQTAKPGVSEKEVYATMLHAMLSNGGELPTLFFLS
                     AGPNLFNSSFVPTDYVIQNGDNLVGEIEAKYGGYAAQAVSPAVVGKPDPAYQAIVEVS
                     HACFEAILAAMKPGVTFGTLFDTYTRTVEGAGPYSWSHPMMHARGLGDDGPALMGDKD
                     LGRFAKIELKAGMTFILKPQVRPTLGKGRASVGDTVTVTANGARRLGKRDMKLVVIDV
                     "
     gene            complement(11821..>12447)
                     /locus_tag="VE170_14495"
     CDS             complement(11821..>12447)
                     /locus_tag="VE170_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16702.1"
                     /translation="KLLNIDSIKSVWDLLDAKWKGKMIGLWPRANYVSTALLFMYHHP
                     QIGPKFLERFYSEMDVTYFSDFRQGTDWLAAGKAPICFLCRLRRAHEQGLPVEEASVY
                     HFKEAPGIGANNGAIALMNNAPHANAAKVFINWYLSREGQIAFRQANNTIEDEITTSM
                     REDLPLEYVPEAARRKKDVNYIEISRHDWMEWKPVGDLINAARQRSGK"
BASE COUNT         2739 a         3720 c         3364 g         2624 t
ORIGIN      
        1 tagtcgatct atgtgtccag tctgacaccg cgccgcaccg aggcagtgaa cacacacgcg
       61 tgcagcgttc acacaagcaa cgactgcaac agatacggcg tcacgacaaa cagcagcgca
      121 gccccgacca cgaccaccgt ccatagccag cgcgccgtgt ttgtaaagtt aggactctgc
      181 cacagcagag gcaatgccaa cgggccgacc aacagcagca tcgtgatcac ataacttttg
      241 gcgtagtaga actccgctgt ctgctcggta gacgtagcga ctttgtaggc atccacgatg
      301 gcgctcaacc agatgagcaa cagggccagt gcggcggtaa tagcgttaaa acccgagagc
      361 ccataagcag cgatgcccag aaccagacag caggcaataa tcgcccagcc ctttttttgt
      421 tctcgattat agatttgccc caggccgggg atgagcatcg ataagagcag ggcgagtaat
      481 gactttgctc gcggggtcat gaccggctac cagatattga aatagcgaac acctgcgctg
      541 tcattctgag ccaaaggcga ggaacctcga tttcctatgt aacagtgaag acaagatcct
      601 tcgcacagct caggatgaca ccatcaattc caagacagcc gatttaatcg ctgcgggtaa
      661 atttcccccc cagcttgctg cgctacgatt ccgatgtagg atgggtggag cggtagcgat
      721 acccatcatt ccgaacggtg ggtatcggcg ctttgcgtct ccacccaccc tacagttcac
      781 ccgaagtgcg ttttcttcat accccgctgc ttgcggcgag gttcttcatt tcaagcgcga
      841 agccttcact ttttctcggc aaacagccgc gaaaacactc tgcgcgcatt gccctcggtg
      901 atattgtaac ggtcctgctc gctcaatccc tcgacggact tgaagatcgg cacaaggtcc
      961 tcgaagtttc gaccggtctc gggatcgatg gcgttgaccg cgccgaacat ttcggtggcg
     1021 aacaggacgc ggtcggcgcc gacccgtttg atcaacaact ccatcgactc tttgtcgtag
     1081 atcgccatgt cccaataaac tcgccgcgcc gcttcttcga agggttccaa tccttcccgg
     1141 acgtgcaggc cgcgttggcg gttccattga taggggatcg ccccgccgcc atgcgggatg
     1201 ataatcttga gcttgggaaa gtccttgaac acccgcgatt tgagtatctc gacagcggca
     1261 gaatggtgat gggcgatgta ataagaattg gtcagatgca tcgccgggtt gagcgacgcg
     1321 ctcgcgtgca cggtgcacgg cacgtcgagc gcgaccatct tctcccacag cggataccac
     1381 cactcgctgc ccatggacgg cgtcagcggt tctttgccgc cggcgacatc gggattgacg
     1441 ttgcagctga tgaaacccaa gtccttgacg cagcggtcca gttcgtcggc ccattctgtc
     1501 gggctcacgc cgggcgattg cggcagttgg cataccggcg acactttgtc gggccaggtc
     1561 ttggcgaccc gcgcgatcaa atcgttgcac gcctcggtcc agtagcggct caccagcggt
     1621 gaaccgaagt gatggcccat ggcgcccgcc tgcggcgaaa acaacaaccg gtcgatgccg
     1681 cactcttcca tccgtttgaa ctgccccgcc atggagcgcg ccaattcttc gtcgctgatt
     1741 tgcagcttgg ggcggatcgg cttggcgaga ttactaatct gctggccgcg gtacgcctgc
     1801 aactgcggcg gcgcggtggt gaagtgcgtg tgtgagtcga tgatcataga aaacctcctg
     1861 agagttcaaa acgttcaagt cgttcaaccg ctgcgctccg ttcaaaacgt tgcagagcgg
     1921 gacagcgctg tcggatcgga tctttcctgg tgaacttcaa ggtacaagac ttcaattgca
     1981 cggattgtca gcttcaaacc gctttgctat accaatctct cacggagaga tcaaaacttc
     2041 tttgccaggc cattccggtc tttcacgttt tgaacgactt gaacctttga acgatttgaa
     2101 cgccttcaag gagttaacat tatgccagac ataccccgcc tcaacggcgt aatcaaagcc
     2161 ctcgaacaag gacaaaccgc cttcgtcgcc tttacacccg tagatatcga gactgccatc
     2221 gccatggcgg gtaccaagct cgacggtgtc gcttttgaaa tggaacacgc gccgctggat
     2281 tttcccggcc tgcgcaacgc tctgcaattc atgctcgacc gcggccagat cgtcgccggc
     2341 ggcacgctcg cgccggcggt cacgccgatg gtgcgcgtgc cgcccaacgg cattgaaatg
     2401 aacagctgga tcgccaagca agtcctcgac atcggcgtca tgggcatcat ctttccccat
     2461 gtcagctccg tcgaagaagc cgccaacgcc gtcggcgcct gccgctatcc gcgcctcaag
     2521 agcgcgccga gatattatcc gccgggcatt cgcggcgacg cgccagcccg cgccgcacgc
     2581 tactggggat tgccgcagca ggaatactac aagcgcgccg acgtctggcc gctggcgccc
     2641 gacggcgaag tcttggtcgt catccaatgc gaagagatgc gcgccatcga aaacctgccg
     2701 aagatattaa aagaagtacc gggcatcggc gtggtcctga tcggcgaagg agacttgagc
     2761 caggaactcg gccacccgcg cgactacgac catcccgtgg tcgccgatgc gatcaataat
     2821 attttgaaaa tctgcaaaga acacaacgtc ccgtgcggcc atccgcaccc ggacgggaaa
     2881 aacatcgagc gcttggtcaa ggacggctat cgcttcatca tgccgtcatc gccgcgctcc
     2941 ttcggcgtcc tcgaccaggg gagaaagcta actggcagaa gctagcaacc agtagttcgt
     3001 cattcccgcg cacgcgggaa tccaggtttt ttatactggc aattgaatcg aacctgaata
     3061 ccggccttcc ggtatgacgg agatggcaaa acactagact tcgaatttct cgcgcagcgg
     3121 tctcagttct tcgcctctgt ctcatccctc tgtcatctcc cctccatcgc ctgcaccgcg
     3181 agcttgaacg cgctgtcgat gtcttcaaac tccggccggg tgccgtcgaa atatttgcgg
     3241 ccgtcgatga gccggttatg catttggata tcgtctttgg gaatatcgat gcgcgccgag
     3301 ttgggcggct catccgggtc gccgagcttt tgcagcgcca tctgaccgcg gcgcgacaga
     3361 taccagttga tgaagacctt ggcggcgttg ggatgggcgc cctggctcgg cgtcgacatt
     3421 gttccgccgc caaccgagaa gctcgcaccc tctttccaca gactggtgtc gaatatttct
     3481 atcggcagtc cttgatgctt cgccttgagg ccgccggagc aaccgaagca gatcgagaat
     3541 cgtccctgcc cgagccagtc gatcatttgc cggctgtcgc ggctgaaggt gatatccttg
     3601 gcgccgaaaa gttggcgaat gaaccctggc ccgagttcgg gatggtaata aaagaactgc
     3661 atggtcgtgc cgatctcggt gcgcggacgg ggatcgaggg cgacgatctt gcctttccat
     3721 ttgggcttga acagatcgta gtaggaggtg aactctttgg cgctgacctg ggcgctgttg
     3781 taatagagct gccccgagct ggcgttggcg agataggcga aaacgtattt attttcggcg
     3841 tcgagatagc ggtgcttgcc gccgtaccag cgcgtggtat cgagcacctc cggcagcatc
     3901 aacgccggct gaatcggctc gagcatcttt gcctgctggt gcaggatgcg gtaattcgac
     3961 gagcccaatc cgaccacgtc ggcgagaaat cgccccgctc tgcgctccgt caatattttc
     4021 gtcgtgatct gggcgccggt gccggtcagc aaataaggcc ggatctccgg gtagtcgctg
     4081 cgaaatacat cgagcacctt gccgtaacgg taaagataga tattgacctt gccttccttc
     4141 ttcgccgcct cgacggtggc atcccattgc ggcgaacgga atgacacctc tgcgctgatc
     4201 gcccgtgatg ccgaacacaa aaaaacccaa gcgatcagta aactggcaaa tcggcattgg
     4261 caattgagaa gtcgcacgcg catcctccca aaacccttac ttgtacttcg aatggcggca
     4321 ctatagcgca gcccggtccc gggtcaataa ttttcccggt tcgcgtagaa ttcagcgcca
     4381 gaacgcaacg actcgacacg cagcctccaa caggtgtaga atcctaaccg agtagaactc
     4441 cgcgcttatc atccctagcg tgaccaaacc ttcattccga tggagcattt ttatgatcca
     4501 acatattcgg agccagaggt tgttgaaaac gagttgctac gccgcgctgc tgcttctgtt
     4561 aacgtcggtg gcactggcgc aaaaccccgg caagattccc cgcgaagtga tcgaccaggc
     4621 ggccgccaac ggcaccgcgt tggtcttggt cggcttgaac gtcccctggc agacagaggc
     4681 gcggctcagc gatgccgaac tgcgcgccca gcgcagcgcc atcgcttcga ttcaaagcga
     4741 ccttttgaat cagctcgaag gcaggaaata caaactcctg cgccgctacc aggaggttcc
     4801 cggtatcgct ttggaagttg gcgcggacgc cctggcggaa ctggcgcgac tgccgatcgt
     4861 gaccaacgtt ttgctcgacc gccccgccgg caccgcgatg ccgccggcga atcgcaatgc
     4921 tgcggcgcgc gccgccggag ccgacctcgc gagcggcgaa aaggtgccgt ccattctttt
     4981 cacccaggcg gcgcgcgacg gcacggtgct ggtgctggcg ggcttgcgca cgccctggca
     5041 gcacgaagaa ggaatgagcg aagaactatg ggcgttgcaa cgccaggcga tccatagcac
     5101 gcagagctac ctgctcgccg agttggccgg cacgcgctat aaggtcatgc ggctctacgg
     5161 caagatcccc gggattgcgc tgcgcgtcgg cgtcgacgcc ttgatcgtct tggaaaaatc
     5221 cccggcgatc accaatgtcg ttttagaccg tcccgctcaa gtcagacgct aatccactgc
     5281 ccaagcaaac tgcggcagcg gaaaattgcg cgccgcacga ttagatgcta tttcacctgc
     5341 atgtccaagt tcgccgaatt ctgcgtccgt tatttcaaac aacacttcga gctccatccc
     5401 accgacgcga tttattacgg cgtcgaaggc tacgatcacc tgctcaacga ttactcggat
     5461 aagagctaca aagccgagaa gatcttcgtc aaagaatcgc tgcgcaagct gggtgagatt
     5521 tcccgggccg agttgactgc cgacgaaagc atcgactacg ccttgctcga agggcggctc
     5581 tcgatccaaa gttatgagca ggccaaggaa gactatcgtc tcaaacaacc ggacatctat
     5641 tcccccaccg aggcgattta tattctcacc gtgcgggcga ccaacgacct cgccgccaac
     5701 ttgttgagcc gcctcaatcg cacgccggcg ctgatccagc agggcattac caacctaaat
     5761 cgcaaagacg ccaatccgcc gaaaatttgg accgagaacg ccatcgaagg cgccaaaggc
     5821 ggcatcacct ttctcgacag cctgcccgag catcccaaag tgcaagccgc ggcaatcgaa
     5881 gcgaacatcc tcgctgccgc catcgaaaag gccaagagcg ccatcgccga tttcgccatt
     5941 tttctcgaac gccatctgct gccgcgcagc aacgccagct acgccgtcgg cgaagagcac
     6001 ttcaatttac tcttgaggaa aaaacatttc ctcgtacacg acgcgcacag cctgctcgct
     6061 ctcggcgaga gcctttttgc cgagaccaag ctggagctcg aagccttggc cgatgagctg
     6121 gcgccgggca agggcgtcga gaccgcggca cgggggattc aggaaaacca cccgtccagc
     6181 gatgccatac tctccgtcta cgatcaggcg atgagggcgg cgcgggaatt cgtgcgcgag
     6241 aagaagctcg tcagcttccc cgccagggaa gaacttcacg tcgtccatac cccggtgttt
     6301 cgccgccacg aaattccctt cgccgcctat ctctcgccgt cgccccaaga tcccgatcag
     6361 gtgggctact actacgtcac gccggtgtcg agcgacgatt tgctgcgcga gcataactac
     6421 gtcggactcg agaatacctc ggtgcacgaa agctatccgg gccatcacct gcaattttcc
     6481 atcgccaact gcacgcccac cgccgcaacc ctgccgcgct tgatgaacga gtcgtcggtg
     6541 ttttatgaag gctgggcgct ctactgcgag caattgatgc aggagcaagg ctttttgaaa
     6601 tccaaggaac accgcttcgt catgctcaaa gaccgccttt ggcgcgctct gcgcatcatc
     6661 atcgacgtca aaactcagac cggcaagctc agctacgatg aagccgccaa cctaatggtg
     6721 cgtgaactgc atttcccgcg cgagcaagcc tgcggcgact tgaactggta cagccagtcg
     6781 ccgtccgtgc cgatgggcta tgcgttgggc tggtcgatca tcaaccgcct gcgcgatcaa
     6841 gaacaaaaac gcctgggcgg caaattcaac ctgtgcgaat tccacgacaa acttttaagc
     6901 agcggcagca tctccttgcc cctagtagaa aaacgacatt tcatgaacta acccgaaacg
     6961 acttgaacgt tttgaaccag ttgaacggct tgaaccaggg gccgcctctc tcgttcaaac
     7021 agttcaattg attcaaaccg tgcaacaatt atgaagaata aagaaaaacc ccgcggcccg
     7081 ttactcggcg cccacatgtc cattgccggg ggcgtcggca acgcctttct cgaaggccgc
     7141 aaagtcgcct gcgaggcgat tcaaattttc accaaatcct cgcgtcagtg ggcatcgaag
     7201 ccgttgtccg aagacgaaat cgcccagttc catgccaacc gcaaggaaac cggcatcgct
     7261 gcggtggtcg cccacgattc ctacttgctc aacctcggct cgccggaccc ggcgttacgc
     7321 aatcgctcca tcatcgcgtt catcgatgaa atggagcgct gcgaagtgct cggcgtgtcg
     7381 aacttgatcg ctcatcccgg tgcccatatc ggcgccggtg aactcgaagg catcaagacg
     7441 attgccaagt cgttggacga agtgcacaca gcgtgtcccg gcttcgcggt caaggtcact
     7501 ctcgaaatca ccgccggcca gggctcgaac ctgggctacc gcttcgaaca aatcggcgac
     7561 atgatggatg cgacgaaaga aaacgaccgg ctgcgcgttt gtttcgacac cgaacatgcg
     7621 ttcgccgccg gttacgacat tcgcaccaaa gaaggctacg agcggacgtt tgccgagttc
     7681 gaccaaacca tcggcatcga gctgctcgcc gcgtttcatt tgaacgattc gaaaaaggaa
     7741 ttccattccc ggatcgaccg ccacgaacat atcggccaag gcttcatcgg cgtcgaagcc
     7801 ttccggctat taatgaacga ccggcgcttc tggggtctgc ccatgtgttt ggaaacaccg
     7861 aagggaccgg atttgaaaga ggaccgcgag aacttgacct tgctgcgcag tctgatcgca
     7921 gtctaagcgg ttttaccctc gccatgccgt gtggagcgag tcgccgagga gaaacgcttc
     7981 atgccggaat ggaaattccc gggaagaacc actgcacggc gagaccgatc agatagccca
     8041 acccgcctgc gctcaccgca accccaaaga actttgcgcc ggaaatcagc acggattggc
     8101 cggcgagata gcctttgaag gcgccgacca gaaaaagggt agccgcgctc aggaaaaccg
     8161 acagggaaag cgccacaacc ccctccgtcg caataaacgg cacgatcggg atgatcgcgc
     8221 cgacgatgaa cgagagtccc atcacgagtg ccgccttaaa cggctgattg atctccgcgc
     8281 tgcccagacc gaaaactttt tcctgaaagg tgcgcatgaa aacgttgcgt tcggcgagca
     8341 gcactttcac catacggtag ctctgttcct tgctcagccc ttcctcgccc aaggctttta
     8401 gcaatccctc cgccgcgatg tagggttcgc gctcggcgag catttccgcg tcgaccagct
     8461 ccttatcgaa tatgtctttt tgcgcgccag aggatagata ggaaccggtg gccatggaga
     8521 tcgcgccgga aaacatcgcg gtgaagccgg tgacgatgac cacgagattg ctttcggtcg
     8581 cgctttggat gccggcgagc agaccgacgg tgctgatcag gccatcctgg atgccgaaga
     8641 caaactcgcg aatccgcgcg cggctttcga tgacgcgctt tttttgttgg atggtgtcga
     8701 gagacatgga tccatggata atgaaaagtg aatgtgacta acggcaatcg gcaattcacc
     8761 gttatcgatt ctgcatggtt ttaatatttc agcgccaaat ccccggtcgc ttcacccggt
     8821 ttccaatccg acgggcacaa gcgctctgtg cgtagagcct tgagcacgcg caaggtttcc
     8881 tcgacgctgc gcccgacatt gacgtggctg acgacttgat agctgatgat gccggccgga
     8941 ttgatgatga acgtcgcgcg caaagccacg ccggcatttt cgtcgagcac gccgtaagcg
     9001 cggctcaacg tcgttgtttc atcgctcagc agcggacaat ccaaaccgcc gagctcggcg
     9061 gaccaagacc tatggctgtc gattgaatcg acgctcacgc cccacaccat ggcatcttcc
     9121 gcggcaaagt ctttcacggc tttgctgaaa ccggtgactt cggtcggaca aataaacgta
     9181 aagtcggccg ggtagaaaaa cagcacgagc catttgctgt tgctggcgcg gagagagtac
     9241 tcgccaacct gatcgcgcag aattcctttg actttgaaat ccggcgccgg ctcgccgatg
     9301 cgcaacaaac tcataagact cccactcgat tcaatcggtt tgccgcctgc ttggttttgc
     9361 cttttagctt ttttattttt gtcttgccaa ctcaaggctt gtgctgccag agcgaaaatc
     9421 cgttctcggc gttggccggc ggcgccgcgg taaaccagga ctcttcctcg ctgccggcct
     9481 tgagcagacc gaaaaaatag atgcgcagca tctgctcgat gagctgggaa atcggcaggt
     9541 cgcgcttggg ctgataccaa gtgtagatcc agttcatcat gccaaagagc gaaaaggtcg
     9601 ccagccgcag atccatggcc ttggcgccgg atgtttcctg caactcgccg agtaaatcca
     9661 tgatcacttt gacatagcgg cgtttgaaca cgaggatctt ttcgtcgaac tcaccggtca
     9721 gggactcgtc ctcatgggcc atgaccttca tctccggcat gttatgcaga aaaaaaccca
     9781 gatgattttc cgcgaacgcg cgtatccgcc cacgcacgtc caagccccgg ctgtcgatct
     9841 gttcccagtg ttcgagcaag gtcacgaagc agcgttcttg aatcagatag agcagctctt
     9901 ccttggtcgt aaagtaataa tacagcccgg acaaactcat cttggtggca cgcgaaatat
     9961 cgcgcaccga agtgtgatga aaaccttttt cggcgaaaat cttcgccgcg gttttgagaa
    10021 tcaattgcag cttttcgtcg taatggcgag ttgcgggttc ggcgcggcta gacatgggtg
    10081 ggagaatact tgcaaatcga ttccgggctg tcaaatgaca gcgagctcgc agatgtggga
    10141 aagaaaggac tcaccgtcga aggaaatgaa gaacacgaag ttcgcaacgt caattttctg
    10201 aagccttcgt gttcttcata ctactatcgc tgatagtttg cgcggcttgc gcaaatttgt
    10261 cgccagacgg tcgccgctcc gcacccctag ggcgaatcat gattcgcccc tacagccgga
    10321 atttgtcttg ggattttatt ttgcgtccct ttgcgttctc ttatgaaaat gccgactagg
    10381 ggcaaaaatc ccccggtcct tcggcccgcc ccctttggca aagggggcca ttgggggatt
    10441 ttccgtcgtt gctttgaacg tggcgcagct cattttcatt tgctgtgttg taccgattgg
    10501 tacatgaatt actttgcgtt ctttgcggcc acttctttta tagttacaca tcgatcacaa
    10561 ccaatttcat atcgcgcttg cccaaacggc gcgcaccgtt tgcggtcacc gtcacggtat
    10621 caccaacgct ggcgcggcct ttgcccaggg tcggccgtac ttgtggtttg agtatgaaag
    10681 tcatgccggc cttaagctca atcttggcaa atcgcccgag atccttgtca cccatcagcg
    10741 ccggcccatc gtcaccgagc ccgcgcgcgt gcatcatcgg atggctccaa gaatatggcc
    10801 cggcgccttc caccgtgcgc gtgtaggtat cgaacagcgt gccgaaagtc acgcctggtt
    10861 tcatggcagc gagaatcgct tcgaaacagg catgcgagac ttcgacgatc gcttgataag
    10921 cgggatcggg tttaccgacc accgccggac tcaccgcctg cgccgcataa ccgccatatt
    10981 tggcttcgat ctcaccgaca agattgtcgc cgttttgaat cacgtaatcg gtgggcacaa
    11041 aagagctatt gaacagattg gggccggcgc tgagaaaaaa taatgtcggc aactcgccgc
    11101 cgttggataa catggcatga agcatggtcg catagacttc tttttcgctc acgcccggct
    11161 tggcggtttg cttcatcgca tcaatggctt tctcgataat cgccgcagag cgctctagaa
    11221 aagacacttc ctctgagctc ttgatggcac gaatctcctg catcatctcg gtggcgttga
    11281 ccagcttggc atggggaaac gcctcttgaa tcgccttgac cgacgtataa ggaattatcc
    11341 cctccggcgc gcgcaccaaa ccggcaagcc cggaaactcc gatggtgcct ttgtcgaggc
    11401 ccaattcttt gatgcgctcg atggcgtttt gactccagcg gttgcgcccg tcgcgcacgt
    11461 ccgccaccca attctgcgcc ttctgccacc agccggcgcg attgaaaaca tatgcagtaa
    11521 cgtcgccctg ggcggggaaa acggtaaaca tctcggtggc gaaaccgccg atgttggtca
    11581 gatagcgcga gtcgccttgc agctgctccc agcgccccgt gttcgccggc agcagcaaca
    11641 catcgatacc gcgctgagcc atctccgcgc gcaccagttg gtaacgccga tctctttctt
    11701 gaatcgataa ctgtggaatc attctgtcct cccgctggtc ggaatctcag atttcatatt
    11761 ccagattacg aattggctcg cacgctagtt gtaattttga atttggaatc tggaatcatc
    11821 ttatttaccc gaccgttgcc gcgcggcatt gatcaaatcg cccaccggtt tccactccat
    11881 ccagtcgtgc cggctgattt ctatgtagtt gacatctttc ttgcgccgcg ccgcttcggg
    11941 gacatactcc agcggcaagt cttcacgcat tgacgtcgtg atttcgtcct cgatagtatt
    12001 attcgcctgg cgaaaggcga tctgcccttc gcgcgagaga taccaattga taaaaacctt
    12061 cgcggcgttg gcatggggcg cattgttcat taaggcaatc gcgccgttgt tggcgccgat
    12121 accgggagcc tctttgaagt gatagacgct ggcttcctcc accggcaaac cttgctcgtg
    12181 agcgcggcgc aggcggcaga ggaaacaaat cggcgctttg ccggcggcga gccagtcggt
    12241 gccctggcga aaatcgctga aataggtcac gtccatttcg ctgtaaaagc gctcgagaaa
    12301 cttcggccca atctgcggat gatgatacat gaacagcaac gccgtcgaga cgtagttggc
    12361 ccgcggccac aagccgatca tcttgccttt ccatttggca tcgaggagat cccacactga
    12421 cttgatcgaa tcgatattga gcagctt
//
LOCUS       DASQKS010000566         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4104301, whole genome shotgun sequence.
ACCESSION   DASQKS010000566 DASQKS010000000
VERSION     DASQKS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104301"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VE170_14500"
     CDS             complement(<1..545)
                     /locus_tag="VE170_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16703.1"
                     /translation="MTIHLLVSWLVSVAGATVFLATTLRAATPEETLAKINHLPAAER
                     HATLVREAKNERSVIWYAPMNRESLREFTSAFEVDYPFLKVEILTGGPQSLLNRIMTE
                     YRAGRYNYDTMNVRSLALYTLKKANAVMRYDAPNRRALRAGFTDKEGYLNGIWASLLV
                     HLYNNKQVPRAQAPKSIDDLL"
     gene            complement(570..1070)
                     /locus_tag="VE170_14505"
     CDS             complement(570..1070)
                     /locus_tag="VE170_14505"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYJ16704.1"
                     /translation="MLKELVLANAYRVTPTGLYLISTAYQGKRNVQFAFRALGLTDDP
                     PLLLIGIQADNFSREVIQQSGEFVVNVCAQNQLQAVDKSRGLSGRTVEDKFAALGLET
                     LPAKHVQAPLVAGCHANVECKLVNSVETDGLYLFVGQALASYIDDQVQPVGRLAGKTF
                     RLDQPI"
     gene            complement(1089..1925)
                     /locus_tag="VE170_14510"
     CDS             complement(1089..1925)
                     /locus_tag="VE170_14510"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HYJ16705.1"
                     /translation="MNDQQKRARLRELLSDSKGAIAPGVTDALFARLAQDCGYGAAHL
                     SGNAIHKNFCLPDRNLLTTTQVAQRIGQISEAADIPLIVDGGSICAETIALTRAVKLY
                     ERSGAAAIRFEDAAVNEYGAAPEELAIAPASVVVDRIKAAVDARRDDAFVVIARCDSR
                     PKESLDQVCQRLAAYAAAGADAVGVQLTEVADFRHIGANVTTPIVTLWPKTQMSAAEF
                     FALGFRIALTPSSIPLAAVNAAREMLIEMHRQGNDRDYFARQKDLGATESWYKTLGAN
                     RK"
     gene            complement(1935..>2249)
                     /locus_tag="VE170_14515"
     CDS             complement(1935..>2249)
                     /locus_tag="VE170_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16706.1"
                     /translation="GFEVQSRSKTPNQATLKMASGQTLVLTEAPFASKGLVMSRKVPG
                     PHIAFYVPTAKWTEAIEQLAKLGIENGDRGAAKEPSADRGGTYMDDPAGYVIQFITDG
                     ME"
BASE COUNT          405 a          636 c          717 g          491 t
ORIGIN      
        1 tgcaagaggt cgtcgatgga tttcggcgct tgggcgcggg gcacttgttt gttgttgtag
       61 agatggacca acagcgacgc ccagatgccg ttcaaatatc cttccttgtc ggtgaaaccg
      121 gcgcgcaggg cgcggcgatt aggtgcgtcg tagcgcatga ccgcattggc ttttttcaga
      181 gtgtatagtg ccaagctgcg cacgttcata gtgtcataat tgtatctgcc cgcgcggtat
      241 tcggtcatga tgcgattgag caggctctgc ggcccgccgg tgaggatttc gactttgaga
      301 aatgggtaat cgacttcgaa ggcgctggtg aattcgcgca gggactcgcg attcatcggc
      361 gcgtaccaga taacgctgcg ctcgttcttg gcctcgcgca ctaaggtcgc atgacgttcc
      421 gccgccggca gatgattgat cttggcgagg gtttcttcag gggtggcggc gcgcagggtg
      481 gtggccagaa aaacagtcgc gccggcgacc gagaccaacc aggaaactaa caggtgaatc
      541 gtcatggcgc tcctccgtaa ccagcgcgat taaatcggtt gatccaggcg aaaagtcttt
      601 cccgccagcc ggcccaccgg ctgcacttga tcgtcgatgt aggatgccag ggcttggccg
      661 acgaaaagat acaagccgtc ggtttccact gaattgacca gtttgcattc gacgttggcg
      721 tggcagccgg cgaccagcgg cgcctggaca tgctttgccg gtaaagtctc caagccgagg
      781 gcggcgaatt tgtcttcgac cgtgcgccct gacaggccgc ggctcttatc gacggcctgc
      841 agttgatttt gcgcgcagac attgacgacg aactcgccgc tttgctgtat cacttcgcgg
      901 ctgaagttgt ccgcttgaat gccgataagc agtagcggcg ggtcgtcggt gagtccgagg
      961 gcgcggaaag cgaactgcac gttgcgtttg ccttggtacg cggtcgagat caggtagagt
     1021 ccggttggcg tgacccggta tgcgttcgct aacacaagtt ctttcaacat cgtttctccg
     1081 cgccagactc actttcgatt tgcaccaagc gtcttgtacc aactctcggt cgcgccaagg
     1141 tccttttgcc gggcgaaata atcgcggtcg ttgccttggc gatgcatctc gatcagcatt
     1201 tcccgggccg cattgacggc ggcgagagga atgctcgatg gggtcagcgc gatgcgaaag
     1261 ccgagggcga aaaattccgc cgcgctcatc tgcgtcttgg gccaaagggt aacgatcggc
     1321 gttgtgacat tggctccaat atgccgaaag tccgcgactt cggtcagttg gacgccgacg
     1381 gcgtcggcgc cggccgcggc gtatgctgcc aaccgctggc aaacctgatc gagggattcc
     1441 ttgggacgtg agtcacagcg cgcgatgaca acgaaagcgt catcgcgccg ggcatcgacg
     1501 gccgctttga tgcggtcgac gaccaccgat gccggtgcga tggcgagctc ttcgggagcg
     1561 gcgccgtact cgttcaccgc cgcgtcttcg aagcggattg ccgccgcgcc ggagcgctcg
     1621 tacaacttta ccgctctggt cagagcgatg gtctcggcgc agatcgatcc gccatcgaca
     1681 atcagcggga tgtcggcggc ttcggagatc tgcccgatgc gttgggcgac ctgcgtcgtg
     1741 gtcagcagat tgcggtccgg caagcaaaag tttttgtgga tggcattgcc ggagagatgc
     1801 gccgcaccat aaccgcagtc ctgagccagg cgcgcgaaca gcgcatcggt gacgcccgga
     1861 gcgatggcgc ctttcgagtc gctgagcagc tcgcgcaagc gcgcgcgttt ttgctggtcg
     1921 ttcatttagt ccggctactc catgccgtcg gtgataaact ggatgacgta gcccgcgggg
     1981 tcgtccatgt aggtgccgcc ccggtcagcg ctgggttcct tggccgcgcc gcggtcaccg
     2041 ttttcaatgc ccagcttggc cagttgctcg atggcctcgg tccatttggc cgtaggtaca
     2101 tagaacgcaa tatgcggtcc tggaactttg cggctcatga ccagtccctt tgaggcgaac
     2161 ggcgcttcgg tcaagaccaa ggtttggccg ctggccattt ttaaagttgc ctgattcggg
     2221 gtcttgctgc ggctttgcac ctcgaagcc
//
LOCUS       DASQKS010000567         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4925933, whole genome shotgun sequence.
ACCESSION   DASQKS010000567 DASQKS010000000
VERSION     DASQKS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4925933"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VE170_14520"
     CDS             <1..546
                     /locus_tag="VE170_14520"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678688.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HYJ16707.1"
                     /translation="EVPVGFKWFSPGLFDGSVCFGGEESAGASFLCKDGSVWTTDKDG
                     IILCLLAAEMIATTGRDAASWYEELTGAMGRPYYTRVDLAASAAQKNALKALKPDALT
                     ATELAGEPIVAKLTHAPGNGAPIGGLKVAAKSGWFAVRPSGTEDLCKIYAESLRSEEH
                     LLAIKDQAAAIMQDVFKAANA"
     gene            558..1376
                     /locus_tag="VE170_14525"
     CDS             558..1376
                     /locus_tag="VE170_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ16708.1"
                     /translation="MQLSIDSKIKLNDGHAIPRLGLGAWMISSGRSCESAVRAALDAG
                     YRHIDTAAYYGNEESVGKAIRDSGIARGEIYLTTKLWNSDHGNPERALERSLGKLKMD
                     YVDLYLIHYPVPERLRSWRALARLRAAGKTHSIGVSNFTVRHLTELLANGDGIPTVNQ
                     VELHPYLYQRELTDFCHEKNIVIEAYSPLTHGERLDDPRLMAIARNYSKSPAQILIRW
                     GLQHDFVVIPKSAKRHRIIENAAVFDFAISAADMQTLDSFNENLRTCWDPTRAP"
     gene            1462..1764
                     /locus_tag="VE170_14530"
     CDS             1462..1764
                     /locus_tag="VE170_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16709.1"
                     /translation="MAQSAPAQETLRVAIPLFPTAAFPLLVAPDRGFFRNEGLTVEPI
                     RINSAPTTYQALISGDVHAVSGAPTGKSIHGAALGQRVRHRADEVSWPAEATAIAS"
     gene            2116..3015
                     /locus_tag="VE170_14535"
     CDS             2116..3015
                     /locus_tag="VE170_14535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HYJ16710.1"
                     /translation="MEAKIKHLIPSIAWPGLLVLCMAIMAYGFTHGHPVLYFNLAYVV
                     LIVTLFSLERWMPHEREWTKPDGQTLANILHTLSSKGTAQLLLQFGGIIGLTELMQDS
                     AHGIWPHSWPLAAQVMLGVVVAEFPLYWAHRLGHEWPVLWRFHAVHHSVTKLWIVNTG
                     RFHFVDSLYKIVLSVALLLALGAPSVVVIWFSAFTGFIGMLTHCNVEMRFVAPLSWIF
                     NTPELHRWHHSKELREANRNYCENVMVWDILFGTYFRESHRRPPADIGIREFMPPRFL
                     HQIIWPFLTEEGKQRIEAGCRTV"
BASE COUNT          706 a          975 c         1024 g          742 t
ORIGIN      
        1 gaagtgccgg ttggcttcaa gtggttttca ccggggctgt tcgacggctc ggtttgcttt
       61 ggcggggaag agagtgccgg ggcgagcttt ctgtgcaaag acggcagcgt ctggaccacc
      121 gacaaggacg gcatcattct ctgtctgctc gctgcggaaa tgatcgcgac gacggggcgg
      181 gacgcggcga gttggtacga ggagttgacc ggcgccatgg gcaggcctta ttacacccgc
      241 gtcgatttgg cggcgagcgc ggcgcagaag aatgctttga aagcgttgaa gccggatgct
      301 cttacggcaa ctgaactcgc cggcgagccg attgtggcga agctcaccca cgcgccgggt
      361 aacggcgcac ccattggggg tctcaaagtc gcggccaaga gcggctggtt cgccgtgcgt
      421 ccttcgggca ccgaggattt atgcaagatc tacgctgaga gcttgcgctc ggaggagcat
      481 ttactggcga tcaaagatca ggcggcggcg atcatgcagg atgttttcaa agcggccaac
      541 gcgtaggtgg gcaggacgtg cagcttagca tcgattcaaa gatcaagctc aatgacggcc
      601 acgccatacc gcgcctcggt ttgggtgcgt ggatgatcag ctcgggcaga agctgcgagt
      661 cggcggtgcg cgcggcgctt gacgccggct accggcatat cgatacggcg gcgtattacg
      721 gcaatgaaga gagcgtcggt aaagccattc gcgacagcgg catcgcccgc ggcgagattt
      781 acctcaccac caagctgtgg aacagcgatc acggcaatcc cgaacgagct ctggaacgca
      841 gcctcggcaa actcaagatg gattatgtcg atctctacct gatccattac ccggtgccgg
      901 agcggttgcg cagttggcgc gcgctggcac gcttgcgcgc agctggcaaa acccattcta
      961 tcggcgtcag caattttacc gtcagacatt tgaccgagct gctcgccaac ggcgatggga
     1021 ttcccactgt caatcaggtg gagttgcatc cgtacttata ccagcgcgag ttaacggatt
     1081 tctgccacga gaagaacatc gtgatcgagg cctatagccc gctgactcac ggcgagcggc
     1141 tcgacgatcc gcgcctgatg gcgatcgcgc gtaactacag caaatcgccg gcgcagatcc
     1201 tgattcgctg ggggctgcag catgacttcg tggtgattcc taaatcggcg aagcgtcacc
     1261 ggatcatcga aaatgccgct gtcttcgatt ttgcaatcag cgcggcggat atgcagacac
     1321 tggatagttt caatgaaaat ctgcggacct gttgggaccc gacccgcgcg ccgtaagcaa
     1381 tcgatcgcta ctggccgagg aggagaagat gaaaaccccg ctgacgttgg gctttgccgg
     1441 tttgatcatg ttgtcggatt gatggcccag tccgcgccgg cgcaagaaac cctgcgcgtg
     1501 gcgatcccgc tgtttccgac cgcagctttt cccttgctgg tggccccaga ccgaggcttt
     1561 tttcgtaacg agggactgac cgtcgaaccg atccgaatca acagcgcgcc gacgacctac
     1621 caggcgctga tctcgggcga cgttcacgcc gtctccggcg cccccaccgg caaatccatt
     1681 cacggtgccg ccctcggcca acgagtgcgg catagggcgg acgaagttag ctggcctgcg
     1741 gaggccacgg cgatcgcgag ctagaaaatt gtcgagctga gagcgaggaa ggagcgggag
     1801 gattgttaag atcgaaatgc ctgccggaaa atttccgact ctttgcttgc caggtgtgtt
     1861 ttcgcgctga ttctagatct gacgaaaggg ccacaccccg agagagagca acatccaaga
     1921 gctgcctgtc tcactctgcc tgtcgtccca gaaaggtagt gcgatgagca gctacccaga
     1981 cgtaaacagg ctcgcctccg cttttctcgc cccctagtca aaaacttcct gcaggatttt
     2041 tttttgatcc tgcttcctgc taaattccga tggcaaaacc tcgaaggtgc gcgtggaaac
     2101 cggggcattt cagtaatgga agctaaaatt aaacacctca ttccgagcat cgcgtggccg
     2161 gggctgctgg tcctgtgcat ggcgattatg gcgtatggat ttacccacgg ccatccagtt
     2221 ttgtatttca acctggccta tgtggtgctg atcgtcacgt tgttttcact cgagcgctgg
     2281 atgccccatg agcgcgaatg gaccaaaccc gacggccaga cactcgccaa tatccttcat
     2341 acgctttcca gcaaaggcac ggcgcagctg ctcttgcagt tcggcggtat cattgggctg
     2401 acggagttga tgcaagactc ggcgcatggc atttggccgc acagctggcc gctggcggcg
     2461 caggtcatgc tcggggttgt cgtcgctgaa ttcccactct actgggcgca ccggctgggg
     2521 cacgaatggc cggttctgtg gcgtttccac gccgtgcatc acagcgtcac caagctttgg
     2581 atcgtcaata ccggcagatt ccatttcgtc gactcgctct acaagatcgt cctcagtgtg
     2641 gcccttttgt tggccctggg cgcgccgtcg gtggtggtca tatggtttag cgcgttcacc
     2701 ggcttcatcg gcatgctgac gcactgcaac gtcgagatgc gcttcgttgc tccgctgtcg
     2761 tggattttca acacgcctga gctgcatcgc tggcaccaca gcaaggagtt gcgcgaggcc
     2821 aacaggaatt actgcgagaa cgtgatggtc tgggatatct tgttcggcac ctatttccgc
     2881 gaaagccacc gccgcccgcc ggccgatatc ggcattcgag aattcatgcc gccgcgtttc
     2941 cttcatcaaa tcatctggcc gtttttgacc gaggagggca agcagcggat cgaggcgggc
     3001 tgtcggacgg tataaggcgt acagcctgcg atctcacgag gcgtatcgca atttctacat
     3061 caggcccgcc gcattctcat ggaagacctg ccgtgccgct atacactctc gcggagagct
     3121 acgacgcgcc gcgaacctta atctggaagg gctcgccgga cggtaagatg cttgcctcgg
     3181 aaatgaaaaa tcgaacctaa gtgactcgac ggacgatcga gctgtcgcgc ggtatttctt
     3241 ggatcgcttt ctccatttct tcccgcatga acgcatgggg ttctattacg acttcgccac
     3301 tcgcaaaatt tttgaccagt ttttttcttg cgcgaaagga cttggccaag aatcaatgca
     3361 tgttaaaatt attagcgaat aacgcggccg atcgcggagg gatcgcgacc ggccgctttg
     3421 gccatgttct tgatgtcgtc aaacatc
//
LOCUS       DASQKS010000568         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3683870, whole genome shotgun sequence.
ACCESSION   DASQKS010000568 DASQKS010000000
VERSION     DASQKS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683870"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VE170_14540"
     CDS             complement(<1..503)
                     /locus_tag="VE170_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16711.1"
                     /translation="MAEGVKARIGFVSGGSSSMPHYNSFLPIVPKEIEIDFQGLQLYG
                     KSLYEIADKKDVILSRIKAFIGEREWDGLILTAAPTEVLNPGLFDDLKAAVSIPFTTA
                     LNACVAALRTYATPKVLLLTPFDAKLNDLIVAHLAKAGVNALSPNSFDRLEVPKKMTP
                     DEVFDLT"
     gene            complement(553..1020)
                     /locus_tag="VE170_14545"
     CDS             complement(553..1020)
                     /locus_tag="VE170_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ16712.1"
                     /translation="MKYWFRCMVVAVGFLAWQSFAFADLPDPKGKTENPNVVAGRKAI
                     EAKDFKSAVGHLTKAVQEIPNDADAHSMLGYSYRKVGTFDKSMEHYQRALKLDSNHRS
                     AHEYLGELYLDLNQPDNADKQLAALKKACPMFGKCEEFEDLKKAIDGYKAKKK"
     gene            complement(1087..1557)
                     /locus_tag="VE170_14550"
     CDS             complement(1087..1557)
                     /locus_tag="VE170_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ16713.1"
                     /translation="MKCFLSAMVLSLALSMVPSIVPAMDTGEPLAKKESASMVAGRKA
                     VEAKDFKSAVGHFTKAVDTEPKNADAYNMLGYSYRKTGTFDKSLEFYQKALKLDAHHR
                     GAREHLGELYLDMNQPDNAEKQLAVLKKDCSFLSKCEEYDDLKKAVDAYKAKKK"
     gene            complement(1631..2569)
                     /locus_tag="VE170_14555"
     CDS             complement(1631..2569)
                     /locus_tag="VE170_14555"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16714.1"
                     /translation="MLLLALLLGIAPKVGAQQGKPLEKVRLTVPAKSLTFVPYYFGKA
                     QGIFAREGIDLEIIVMRPPLGVTALLAGELDYSAAGGLSVRAALKGAPLRTITFIQTR
                     LSFSLIGQPSMAAARIKNVSVSGIGSLAHYAAQAMMKRLGNEKVAYVSTNTTANSYTA
                     LLGKATDAVILTPPYTSMATLAGYADLGNTYDLRDLQGGLVARLSSIHEQRERVKAVI
                     RGTLRSMEAIVKNDNEVVAYLQKDFGLEPRIAADTSKIIKQLVNADGDLEEPVLQSII
                     DKLRAESGIAAEIPSERLVDMSLLREVRAELKKRAP"
     gene            complement(2824..4248)
                     /locus_tag="VE170_14560"
     CDS             complement(2824..4248)
                     /locus_tag="VE170_14560"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16715.1"
                     /translation="MHYRDLRGWLDGVDSFGELRVVEGADWNQEIGAVAEVAAQTNPP
                     PAVLFDKIKDYPAGRRVLTGVHNPTLKRQCLTNHLPLDYTRDQFIDAWKKRLDRPILL
                     PPRVVDRGPVLENVFEGKDIDLLSLPVPWWHEGDGGRYIGSMDTTISRDPEEGWINVG
                     CYRVMVHDKDTLALYISPGHHGHIHRQKYFDRGQPFPVAISFGPDPLLWFFSQMEIPA
                     GESEYDYAGGVRGEPYEVILGKHTGLPIPAYSEIVIEGEVLPGTSIPEGPFGEFTGYY
                     AGGLRAEPMLKVKRLMYRNDPIMTGAPPFKPAPGAENNLIRAAYIWNYLDKAGVPDVR
                     SVAYHQTRLLVALSIKQRYPGHARQAALIASQCRAAALLTRYVMVVDDDIDIWDSNEL
                     LWALCTRTDPAKDIDILRRCWSNPIDPIIPPSERGFQSRGIIDACRPYEWINEFPKVS
                     GASKELKEKVTKKFAASLAGKKET"
     gene            4401..>4787
                     /locus_tag="VE170_14565"
     CDS             4401..>4787
                     /locus_tag="VE170_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HYJ16716.1"
                     /translation="MPKSHRNRLALAATLAALWSCATAADNPSQRYAAGTGPADQLRL
                     PAPFDTPSARNQSKVIGWPQGRTPKAAPGFDVSLFADKLANPRLAYVLPNKDVLVVES
                     IREWPGREDRPDRSVNRITLFRDVDND"
BASE COUNT          967 a         1473 c         1260 g         1087 t
ORIGIN      
        1 ttggttaaat cgaacacctc gtccggcgtc atcttcttcg gcacctcgag ccgatcgaaa
       61 gagttcggcg acagcgcgtt cacgccggcc ttggcgagat gggcgacgat caggtcattc
      121 aactttgcat caaagggggt cagcaacagc actttcggag ttgcataagt acgcagcgcc
      181 gcgacgcaag cattcagcgc cgtggtgaaa gggatcgaga cggccgcttt caaatcatcg
      241 aacaatcccg gattcaacac ttccgtcggc gcggcagtga gaatcagacc atcccattcg
      301 cgctcgccga tgaacgcttt gatccgactg agaatcacat ccttcttatc ggcaatctcg
      361 tacagcgact tgccgtagag ctgcagcccc tggaaatcga tttcaatctc tttgggtacg
      421 ataggcagga agctattgta gtgcggcatc gagctcgacc cgccgctgac gaaaccaata
      481 cgtgctttta caccttcagc catcctatcc tccgagcgat cctcgatcct cgattctcac
      541 ttctcgaccc ggctatttct tcttcgcctt atacccatcg atggccttct tcaaatcctc
      601 gaactcttcg cacttgccga acatcggaca ggcttttttc aacgccgcca attgcttatc
      661 cgcgttatca ggctgattca gatcgagata aagttcgccc aagtattcat gggcggagcg
      721 atggtttgaa tcgagcttga gcgcccgttg ataatgctcc atggatttat cgaacgtccc
      781 aaccttccgg tagctgtagc cgagcatgct gtgggcgtcg gcgtcgttgg ggatttcctg
      841 gaccgctttg gtcagatggc cgaccgcgga tttaaaatcc tttgcttcga tggctttacg
      901 ccccgcgacg acgttgggat tttcagtttt tcctttcgga tcgggcaggt cggcaaacgc
      961 aaacgactgc catgccaaga acccgaccgc gacaaccatg caacgaaacc aatacttcat
     1021 agaacctcca tttgagttgg gcagaaaact cttgtctttc tccgtctgtc gtctctgtct
     1081 tcttcgctac ttcttcttcg ccttataggc atccaccgct ttcttcagat cgtcgtactc
     1141 ttcacacttg ctaaggaaag aacagtcttt tttcaacacc gccagctgct tttcggcgtt
     1201 gtcgggctgg ttcatatcga gataaagctc gcctaggtgt tcacgggcgc cgcgatgatg
     1261 ggcgtcgagc ttcaaggcct tctgataaaa ctccagagat ttgtcgaagg tgccggtctt
     1321 ccgataactg tagcccagca tattataggc gtcggcgttt ttgggctccg tgtccacggc
     1381 tttggtaaaa tggcccacag ccgatttgaa atcttttgcc tccaccgcct tacgcccggc
     1441 gaccatactg gcactctctt tttttgccag gggttcgccg gtatccatgg ccggcacaat
     1501 cgacggtacc atcgacaaag ccaatgacaa caccatcgca cttagaaagc atttcattta
     1561 aacgaaaatc tcccggcaat atttgccgac atcttaactt tgccccggaa ccgacgcaag
     1621 atcgtgcgcg ttacggcgca cgtttcttta actcggctct aacttcgcgc aacagcgaca
     1681 tatccaccaa ccgctccgac ggtatctccg cggcaatgcc ggattccgcg cgcaatttat
     1741 cgatgatcga ttgcagcacc ggctcttcga gatcgccgtc ggcgttgact agctgtttga
     1801 tgatcttcga ggtatccgcg gcgatcctgg gttccaggcc gaagtctttc tgcaaatagg
     1861 ccaccacttc attgtcgttt ttgacgatcg cttccatgct gcgcaaggtc ccgcggatga
     1921 ccgccttgac ccgctcgcgc tgctcgtgaa tcgacgacaa gcgcgccacc aaaccgccct
     1981 gtaagtcacg cagatcgtag gtgttaccga gatcggcgta gcccgccaac gtcgccatcg
     2041 aagtatacgg cggcgtcagg atcaccgcat cggtggcttt accgagcagc gccgtatagc
     2101 tattggccgt ggtattggtc gaaacatagg cgaccttctc attgcccaag cgcttcatca
     2161 tcgcctgcgc ggcgtagtgg gcgagggaac cgataccgct caccgaaacg tttttgatcc
     2221 gcgccgcagc catgcttggc tgcccgatca ggctgaaact caaccgcgtt tgaataaatg
     2281 taatcgtgcg caacggcgca cccttcagcg cggcgcgcac cgacaggccg ccggccgcgg
     2341 aataatcaag ctcgcccgcc agtaacgcgg tgacgcccag aggcgggcgc atgacgatga
     2401 tctctaggtc gatcccttcc cgcgcgaaga tcccctgcgc cttgccgaaa taatacggca
     2461 cgaaagtcag ggacttcgcc ggcaccgtga ggcggacttt ttccagcggc tttccttgtt
     2521 gggcgccgac cttaggcgcg atccccagta gaagtgccag cagaagcaac ttcgctgcac
     2581 cttttgtcca tcgcaagggc gacccgccgg tcgtccctac accaccgctg ccgataatca
     2641 tgtatggaat cccatcagtt cgaatgtcgt tagcagagcc ccgccactgc catccccagg
     2701 gcgaatcatg attcgcccct acgtcagatt tcctcttgaa atttttttgc tttcattgcg
     2761 tactttgcgg tcagtcatct gattccgaat tttcactatt ttgtgatcta tgtgcctttg
     2821 tggttaagtc tctttcttcc ccgctagact cgcggcgaac ttcttcgtga ctttttcttt
     2881 cagctctttg ctcgcgcccg agactttggg aaactcattg atccattcat agggccggca
     2941 ggcgtcaatg atgccgcggg attggaagcc gcgctcactc ggcgggatga tcgggtcgat
     3001 gggattgctc cagcagcggc gcaggatgtc gatgtccttc gccgggtcgg tgcgcgtgca
     3061 cagcgcccac aagagctcat tgctgtccca gatatcgata tcgtcgtcga ccaccatgac
     3121 gtagcgcgtg agcagcgccg cggcgcggca ttgcgacgcg atcagcgccg cttgccgggc
     3181 atggccggga tagcgttgct tgatcgataa ggcgaccagc aagcgggttt gatgataggc
     3241 gacgctgcgc acgtccggca cgccggcttt atcgagatag ttccaaatat aggcggcacg
     3301 gatcaaatta ttttctgccc ccggcgccgg tttgaacggc ggcgcgccgg tcatgatcgg
     3361 atcgttgcga tacatgagcc gctttacttt gagcatcggc tcggcgcgca gaccgccggc
     3421 gtagtaaccg gtaaattccc cgaacggccc ttcggggata ctcgtgccgg gcaacacttc
     3481 gccctcgatg acgatctccg aataggcggg aatcggcagg ccggtgtgtt tccccagtat
     3541 cacttcatag ggttcgccgc gcacgccgcc ggcgtagtcg tactccgact cacccgcggg
     3601 aatctccatt tgactgaaga accacagcag cgggtcagga ccgaaactga tcgccacggg
     3661 aaaaggctgg cccctgtcga aatatttttg ccggtgaata tggccgtgat ggcccggtga
     3721 gatgtagagc gctaacgtgt ccttgtcatg caccatgacc cgataacagc cgacgttgat
     3781 ccaaccttcc tcggggtccc ggctgatggt cgtgtccatg gagccgatgt aacggccgcc
     3841 gtcgccctca tgccaccacg gcacgggcaa agacaacagg tcaatatcct tgccttcgaa
     3901 aacattttct aacaccggac cgcggtcgac gacccgcggc ggcaacaaga tcggccggtc
     3961 gaggcgcttc ttccatgcgt cgatgaactg atcgcgcgtg taatccagcg gtaaatgatt
     4021 ggtcaagcat tggcgcttga gcgtcggatt gtgcacgccg gtgagcacgc gccgtcccgc
     4081 cgggtaatct ttgatcttat cgaacaacac cgccggcggc gggttggtct gcgccgcgac
     4141 ctcggccact gcaccgatct cctggttcca gtcggcgcct tcgaccaccc gcaactcgcc
     4201 aaacgaatcg acaccatcta accaaccgcg cagatcgcga taatgcattc cgcctccttc
     4261 gaaccgtggc gatcttagaa cggaccctgc cgtggtacaa ccttcacttt cccgctttcg
     4321 cgcagtaagt tatttgtaat agagtcgggc accgatcctg gcgatgagcc cgcccgataa
     4381 ttcattacag gagtagattc atgccgaaat ctcatcgcaa tcgcttggcc ctcgcggcca
     4441 cccttgccgc gctttggtcc tgcgccacgg cggccgacaa tccgtcgcag cgctacgccg
     4501 ccggcaccgg cccggccgac caacttcgtt tgcccgcgcc tttcgacacg ccgtcggcgc
     4561 gcaaccagtc aaaagtgatc ggctggcctc aggggagaac gcccaaggcc gcgcccggat
     4621 tcgacgtcag tctattcgcc gacaagctcg ccaatccgcg cttggcttat gtcttgccga
     4681 acaaagatgt cctcgttgtc gaaagcattc gcgagtggcc aggccgcgag gaccgcccgg
     4741 acagatcagt caatcgcatc accttgttcc gcgacgtcga caacgac
//
LOCUS       DASQKS010000569         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1981197, whole genome shotgun sequence.
ACCESSION   DASQKS010000569 DASQKS010000000
VERSION     DASQKS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981197"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VE170_14570"
     CDS             complement(<1..161)
                     /locus_tag="VE170_14570"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HYJ16717.1"
                     /translation="MARYGKKAAQKVKRAMHERKKGTLKSGRSGKTVKSRKQAIAIGL
                     SEARRAGGKV"
     gene            421..1215
                     /locus_tag="VE170_14575"
     CDS             421..1215
                     /locus_tag="VE170_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16718.1"
                     /translation="MKTASGIFSSRSAAEKAMRELLGLGLTEDQISLLVPGKQNGEQD
                     TVATEDGEQPGMGKVIGGVVGGALGLSGGMPLGMVVSSLLFPGVGPVLAIGFLTAAIA
                     GSIGAAGGAAAGGALEYSLTEGLPRDELYFYEDALRQGRTILILRTEDDQQLEAGRKI
                     LTDAGAESIDAAREKWWIGLRDAEAESYDPSENFLKVESTYRKGFEAALDPSNRSRSY
                     TEARNDLKRHFPNLWEAHEFRAGYERGHQHAQTRLRQSEINTGAGT"
     gene            complement(1276..3285)
                     /locus_tag="VE170_14580"
     CDS             complement(1276..3285)
                     /locus_tag="VE170_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ16719.1"
                     /translation="MAASDQEEAQLRSVALQNARSIDLARRRAEEALRRQSDWLRVTL
                     SSIGDGVISTDIDGRVSFMNGIAEVMTGWTLNEALGRALDDVFRIINESTREEAENPA
                     ARVLREGAMVGLGNHTILIAKDGTERPIDDSASPIRDDAGHISGCVLVFRDVTERRRA
                     ERDLSRSERELAEFFENANVGLHWVGPDGIILRANQAELDLLGYHREEYVGRHIAEFH
                     ADRPVIDALLTRLTAGESLHEYPVRLRCKDRSIRHVLLNSSGFFDNGKFIHSRCFTHD
                     ITERQRTEEMLAEANRRKDEFLATLSHELRNPLAPIRNAVQILKTISPATSTLEWCRD
                     LIDKQVSQMARLMEDLLDLTRITRNTLELRKQPADLTAVIEGAIETSRPLIEAGRHEL
                     TINLPAQKMMLDGDAARLMQVFANLLNNAAKYMEADGHIHVTAVVRRAAQDGLQEVIV
                     TIKDNGIGIASELLPHVFEMFFQADRSKERHYGGLGIGLTLARHIVEIHGGAIEVKSE
                     GLGNGTEFVVRLPLAKEPVERDQYRVASARHTSLRSQNLSRSVLVVDDNKNQVESIAM
                     LLKILGCEVRAAHDGLSALEALKEFVPEFALIDIGLPGIDGFELARRIRQVEEFNKIT
                     LIAQTGWGREEDRAESRQAGFNYHLVKPLDFQLLEKILTEVPRKT"
     gene            complement(3285..4358)
                     /locus_tag="VE170_14585"
     CDS             complement(3285..4358)
                     /locus_tag="VE170_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ16720.1"
                     /translation="MTKALTIPTEPIGSIPRPPGLIDAIIKRGGNDPSLEPLFDDAIR
                     DTIARFEATGSPVISDGEQRKYHNFWTYSVQGLANTAADGFKIPFVAGHTRRMPRLTG
                     GPFRYKNYADSYLDFALRYAHVPVKQAVISPSALSLMYPPDGIPDYPRDQFIDDLLTE
                     HETEVRACLRKGAHKVQIDFTEGRLASKIDSSGVLLQSFIDLNNLALARFSAADLQRI
                     GVHTCPGGDLDSTHSADVDYAELIPQLFGLMVGNFYIALAGERDRVRVLKIIREHMRA
                     NQRIFVGVIAPIDKRVETAEQVRDRVLEAAQYIPLDQLGTTDDCGFSPFCDDTSTTRE
                     TAFAKIRARVQGTALAAEILGGH"
     gene            complement(4418..>5288)
                     /locus_tag="VE170_14590"
     CDS             complement(4418..>5288)
                     /locus_tag="VE170_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ16721.1"
                     /translation="VFANLLNNAAKYMEGNGKIRLKASLERTLTVAPFEKLRIESAEN
                     GEVGKLEAAGLDAATTGEVVISIKDTGIGLAPEKLLHVFDMFFQVESGKERSYDGLGI
                     GLTLARNLVELHGGTIEARSDGPGKGSEFIVTLPVARVDGGRAASERTETQGMSALPA
                     KRVAVVDDNNLQARSMATLLEMMGYQARVAYDGESAMRMIAEFVPQVALVDIGLPGLD
                     GYELARRLLEMPQARGITLIAQTGWGRDEDRELSRQAGFQHHLIKPIDHQLLEKLLGE
                     IFGAMSREASVSE"
BASE COUNT         1107 a         1522 c         1448 g         1211 t
ORIGIN      
        1 actttaccgc cggcgcgacg cgcttcggac aagcctatgg cgatggcttg cttacggctt
       61 ttcaccgttt tgccagatcg cccactcttc agagtccctt ttttacgttc atgcattgcc
      121 cgttttactt tttgcgcagc ttttttaccg taccgtgcca ttagcgcacc tccatttcat
      181 gactgagcat aaaaagctgc tgcaattggt attccgatta gaaaaatgcg cgcgctaaga
      241 cgattcactt atgatgtccc attattcgga aggtgaaggc gaaagcggcc cacacatggg
      301 cgaagatttt ttcacggagc tcaccttaat aacgtacgca acgcttcacc aaaccgcttt
      361 ggcactttga ttgctgtttt taggcttgtt gatggcacag gtaaaaatag gagacatgat
      421 atgaaaactg ccagtggaat attttcttcg cggagcgctg ctgaaaaggc aatgcgcgag
      481 cttctcggac tgggtcttac cgaagatcaa ataagtctcc tggttccagg caagcagaat
      541 ggtgaacagg acacagttgc aacagaggat ggcgagcagc ccggtatggg caaggtcatc
      601 ggcggagtgg ttggcggggc gctcggttta tcgggcggta tgccgcttgg catggtagtc
      661 tcatcactac tgttccccgg agtggggccg gtgctcgcca tcggttttct aaccgcggcg
      721 atcgccggca gcattggcgc cgctggaggt gccgccgcag gcggggcgct ggagtattcg
      781 ttaaccgaag gactgccgcg cgatgaactg tatttctatg aggatgcgct gcgccagggt
      841 cgaacaattc tgattctccg caccgaagac gaccagcaac tcgaagccgg gcgaaagatc
      901 ttgactgacg ccggggcgga aagtatcgat gccgcccgag agaaatggtg gatcggtctg
      961 cgcgatgccg aagcggagtc ctacgatcca tctgagaatt ttctgaaggt cgagtcgacc
     1021 tatcgcaaag gctttgaagc ggctttggat ccgtcgaacc gaagcagaag ctacacggaa
     1081 gcccggaacg atttgaagag gcactttccc aatctatggg aagcgcatga gtttcgtgcc
     1141 ggctatgagc gaggtcatca gcatgctcaa acccggctac ggcaaagcga aataaacacc
     1201 ggcgccggaa cctagtgcac agggccgatt cacaaacgca atatcgacct cgcgcaactg
     1261 cgccgtaggt aagcctcaag tcttgcgcgg tacttccgtt aatatctttt caagcagctg
     1321 gaaatctagc ggttttacga ggtgataatt aaaccctgct tggcgcgatt ctgcacggtc
     1381 ctcctctctg ccccaaccgg tctgagcgat tagagtgatc ttgttaaatt cctcgacctg
     1441 gcgaatccgg cgcgcaagct cgaaaccatc tatgcccggt aaaccaatat cgatcagagc
     1501 gaactcggga acaaattctt tgagagcttc gagggcgctc agaccatcgt gagccgcgcg
     1561 cacttcgcaa ccaagaatct tcagcagcat ggcgatgctc tcaacctgat ttttattgtc
     1621 atcgaccacc agcaccgagc gcgacaaatt ttgtgaacgc agactcgtgt gtcttgcgga
     1681 ggcgacgcgg tattgatcgc gttcgaccgg ttctttggcc aacggcaggc gcacaacaaa
     1741 ctcagtgccg ttaccgagtc cttcactttt gacttcgata gcaccgccgt gaatctcgac
     1801 gatatgacgc gccaaggtaa gtccgattcc caagccgccg tagtggcgtt ctttgctccg
     1861 atcggcttga aaaaacatct cgaaaacatg aggcagcaat tccgacgcga tgccgatgcc
     1921 gttatctttg atcgtaacaa ttacttcctg gagtccgtcc tgcgcggctc gacgaaccac
     1981 cgccgttacg tggatatggc cgtcggcttc catatacttt gccgcattat ttaatagatt
     2041 ggcaaatact tgcatcaatc gagcggcatc gccgtcgagc atcatttttt gcgccggcag
     2101 gttgatcgtc aactcgtgcc ggcccgcttc aatcaacggc cggctggttt cgatcgcgcc
     2161 ttcgatcacc gccgttaaat ccgccggctg tttgcgcagt tctagggtat tacgcgtgat
     2221 ccgggtgaga tcgagcagat cttccataag tcgcgccatc tgggagacct gcttgtcgat
     2281 caaatcccgg caccattcga gcgtcgatgt agcggggctg atcgtcttta aaatttgcac
     2341 ggcgttacga atcggcgcca gggggttcct caactcatgg gataacgttg cgagaaattc
     2401 atccttgcgg cgattggcct cggcgagcat ttcctcggtt cgttgacgtt cggtgatatc
     2461 gtgcgtgaag cagcggctgt gaatgaactt accgttgtcg aaaaaaccgc tggagtttaa
     2521 cagcacgtgt cggatcgatc ggtctttgca acgcaatcgc accgggtatt cgtgcaggct
     2581 ctcgcctgcg gtcaagcgcg tgaggagagc gtcgatcaca ggccgatcgg catgaaactc
     2641 ggcaatatgg cggccgacgt attcttcccg atgataaccg agcagatcaa gctccgcctg
     2701 attcgcccgc aggatgattc cgtccgggcc gacccaatgc aggccgacat tggcgttttc
     2761 gaagaactct gcgagttcgc gttcgctgcg gctcaggtcg cgctcagccc tgcgccgctc
     2821 ggtcacatcg cgaaacacca agacgcatcc gctgatatgc cccgcatcat cgcggatggg
     2881 cgaagcgctg tcgtcgatcg gtcgttcggt gccgtctttg gcaataagaa tggtatgatt
     2941 ccccaggccg accattgcgc cttctcgcag cacccgggca gccggatttt cagcttcctc
     3001 tcgggttgac tcattgatga tacggaatac gtcgtcaaga gctcgtccca acgcttcatt
     3061 caaggtccat cccgtcatga cctcggctat accattcatg aaactcacgc ggccatcgat
     3121 gtccgtgctg atcacgccgt cgccgatgct ggaaagcgtg acgcgcagcc aatctgactg
     3181 cctgcgcagc gcttcttctg cacgccggcg cgcaagatcg atgcttcggg cgttttgcag
     3241 agctacagaa cgaagctgtg cctcctcctg atctgatgcc gccattaatg acctccgaga
     3301 atttccgctg caagtgccgt gccctgcacg cgcgcgcgaa tctttgcaaa ggccgtttcg
     3361 cgggtcgtcg aagtgtcatc gcagaacggt gagaaaccgc agtcatcggt ggtgcccagt
     3421 tgatcgagcg gaatgtattg ggcggcctcg aggactcgat ccctaacttg ttcggctgtc
     3481 tcgacacgct tatcgatcgg ggcgataaca ccgacgaaaa ttcgctggtt ggcccgcatg
     3541 tgttcgcgga tgatcttcaa cacccggacc cggtcccttt cccctgccag cgcgatgtag
     3601 aagtttccca ccatcaggcc aaacagttgc ggtattagct cagcgtaatc gacatccgcg
     3661 ctatgagtcg aatcgaggtc gccaccggga caggtgtgca cgccgatgcg ctgtagatct
     3721 gcggcggaga agcgcgccaa cgccagattg ttcagatcga taaagctctg caaaagaact
     3781 ccggaagagt cgatcttgga tgccagccgt ccttcggtga agtcgatctg cactttgtgc
     3841 gcgcccttac gcaagcaggc gcgcacctcg gtctcgtgct ctgtcagcag atcgtcgatg
     3901 aactggtcgc gtggataatc aggaatcccg tcgggcggat acataagact gagggcggaa
     3961 ggcgagatga ccgcctgctt tactggcaca tgggcatagc gtagggcaaa gtccaggtaa
     4021 ctatccgcat agttcttgta gcgaaaagga cctccggtca agcgcggcat gcggcgggtg
     4081 tgaccggcga cgaacggtat cttaaagccg tccgctgcgg tattggccaa tccctggacg
     4141 ctgtaggtcc agaagttgtg atactttctc tgttcgccgt ccgagatcac cggcgacccc
     4201 gtcgcctcga agcgggcgat ggtgtcgcgg attgcgtcgt caaagagcgg ctccaatgac
     4261 gggtcattac ccccccgttt gataatcgca tcgatcagcc cgggcggtct cgggatgctg
     4321 ccgatgggct cagtcggaat tgttagggct ttcgtcaatt gtatcttcct cctgcgtcgg
     4381 caaaaagttc tttgaccgct tgtcgatcgt ttgttctcta ctccgagaca ctcgcctccc
     4441 gcgacattgc accgaaaatt tcgcccaaga gtttttctag aagctggtga tcgatcggct
     4501 tgatcagatg gtgctggaat ccggcctggc gtgacaactc ccggtcttca tcccttcccc
     4561 aaccggtctg ggcgatcagc gtaatgccac gcgcttgcgg catctcgagc aaccggcgcg
     4621 ccagttcata tccgtctaat cccggcaatc cgatgtcgac gagggccact tgcggaacga
     4681 actcggcgat catgcgcatc gcgctctcgc catcataggc gacgcgtgct tgataaccca
     4741 tcatctcgag cagcgtcgcc atggaccgcg cttgcaggtt attgtcatcc accacagcca
     4801 cgcgcttggc aggcaatgcg gacatgcctt gcgtttccgt ccgctcgctt gctgctcgac
     4861 cgccatcgac tctcgccacc ggcaaggtca cgataaactc gctacccttg cccggcccgt
     4921 cgcttcgcgc ctcaatcgtg ccgccgtgca gttcgaccaa gttacgcgca agcgtcaacc
     4981 caatccccaa gccatcgtag ctgcgctctt tgccgctctc aacctgaaaa aacatatcga
     5041 atacatgcag cagtttctcg ggcgcgaggc ctatgccggt gtctttgatc gaaataacga
     5101 cttcgccggt cgttgccgcg tcaagtccgg cggcttctaa cttcccaacc tcgccgttct
     5161 cggcggactc tatcctgagc ttctcgaagg gcgcaacagt cagagtcctc tcgaggctgg
     5221 ctttgaggcg aatcttgcca ttcccttcca tgtacttggc ggcgttgttc aataaattgg
     5281 caaatact
//
LOCUS       DASQKS010000570         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4928883, whole genome shotgun sequence.
ACCESSION   DASQKS010000570 DASQKS010000000
VERSION     DASQKS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4928883"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VE170_14595"
     CDS             complement(<1..999)
                     /locus_tag="VE170_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16722.1"
                     /translation="MATFNGELGLQFVTHLANHYRLRDLVRLAQLGADSGFCQIWVND
                     NIRYRNQMVVLTAIASRVPVRLGTAIMVPYFHNPLDMADAFAALSELCEGREIGIGIA
                     RGDLGQSPQHVQALKPIAMVSETTRLLRRALTGEEFPYSDYPFLTEFYRLNPKGKFKL
                     AFTPRTDFKFYGGGNGPQALGLCGRIMDGVISSGTYIPMLKAGRLEAMLATADSAARA
                     EEPGKCLRKVCELNVSIAANREQALDFPRRQVAHSILQWEALGFTPDEYAKMGVAREQ
                     VLKLKEAFESGATVEQAATLVTDHMVRCYYAAGKPEEVRDQIIELVGAAGRLGYDHV"
     gene            complement(1182..2126)
                     /locus_tag="VE170_14600"
     CDS             complement(1182..2126)
                     /locus_tag="VE170_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ16723.1"
                     /translation="MAVIISEAQTRKLIDMSQAVNLVDKMFRDRAAGKMRNVPRRRLK
                     GSSKQLNMMAAWNQDSDLICLRSYAAGANIITLFHGGKGGIQGIVNMAFLSSLRTGAA
                     TGVAAKYLAPADSKILGIIGPGWQATFQIEAVMEACDIEQIVVWGRTPKRRKDFVKQM
                     SKVVKKVDWKEVDSIDECEGLSDILVVSTDSATPVATGNNLKPDVLVASIGANSTVKH
                     EVSNSLIRRMDLIVTDDIAAARADSGDLVEACQTRIARWEDILPLEKFVAGGAPQPRP
                     KRIFFQSNGIADEDLAVGKFVLDQAKRKKIKVRNVTEF"
     gene            2328..3032
                     /locus_tag="VE170_14605"
     CDS             2328..3032
                     /locus_tag="VE170_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16724.1"
                     /translation="MAISNIQTRLCPHCANSIALDTLTCPYCRASLMPVPVPQWPSHD
                     KESPSADVTPRKSAIPLGSKLILVLGLVVFALGVFLVGGHSERSTLEPILEVKEKALQ
                     ASEQKVKTLEAQLVQWDKALRASESQLEEFKTKLNAQEKSLAAAQQKRKDAEREVNRL
                     ASRPAPAPSPVRSRAQNPALPPPPPTPVARRIVEPGLYETVRATDVYEEPLASSRVLN
                     RVGGGTEITVVRGLGQ"
     gene            3251..>4164
                     /locus_tag="VE170_14610"
     CDS             3251..>4164
                     /locus_tag="VE170_14610"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16725.1"
                     /translation="MKEPATIQSDAPVMAQGFLAKTFTSLSHRNYRYLWFGTVFMSAG
                     QWIQQVTLGWIVYDLTGSSVLLGVLNGLRALPFLVASPIAGVFADRMDRRQLILGCEC
                     FLALLTLLMGVVVVSGKLAVWHLFSFTLLTGVAWAFVDPIRQSLVPVLVPKPDLMNAV
                     ALNSAAFNMTKVIGPSLGGLLIVAFGAAGNFFVQGAAYAVVVLIVYRMVVPATPGEAR
                     RSSPLANLKEGLSYVWTNPIVFALMAAALVPRVFAMPFQTLMPVFQKDVLQVGPEGLG
                     MLLAAPGLDALLAGLALATLSSRIKRQGV"
BASE COUNT          787 a         1167 c         1270 g          940 t
ORIGIN      
        1 gacatggtcg tagcccaatc ggccggcggc gccgaccagt tcgatgatct gatctctgac
       61 ttcctcgggt tttcccgcgg cgtagtaaca gcgcaccata tggtctgtga ccagagtggc
      121 ggcttgctcg accgtggcgc cggactcgaa ggcttctttg agcttcaata cttgctctct
      181 cgccacgccc atcttggcgt attcgtccgg ggtgaagccc aacgcttccc attgcaggat
      241 cgagtgcgcc acttggcgcc ggggaaaatc taaggcttgc tctcgattcg cggcaatcga
      301 aacattcaat tcgcaaacct tgcgcaaaca tttcccaggt tcctcggctc gtgccgcgct
      361 gtcggcggtg gcgagcatgg cttcgagccg gccggctttt agcatcggga tgtaggttcc
      421 cgaactgatc acgccgtcca tgatgcggcc gcacaggccc agcgcttgcg gcccgttgcc
      481 gccgccgtaa aatttaaagt cggttcgtgg cgtgaaagcg agcttgaatt tgcccttggg
      541 attgaggcgg taaaattccg ttaaaaaggg atagtcggag tagggaaact cttcgccggt
      601 gagcgcacgg cgcagcagcc gggtggtctc gctgaccatg gcgatgggct tgagcgcttg
      661 gacatgctgc ggcgattggc cgaggtcgcc gcgggcgatg ccgatgccga tctcgcggcc
      721 ttcacacaat tccgagagcg cggcaaacgc atccgccatg tccaagggat tatggaagta
      781 cggcaccatg atcgctgtgc ccaggcgaac cggcacgcgg gatgcgatgg cggtcagcac
      841 taccatctgg ttgcggtagc gaatgttgtc attgacccag atctggcaga agccgctatc
      901 ggcgccgagc tgagccaggc gcacgaggtc acggagtctg tagtgattgg ccaaatgggt
      961 gacgaattgc aggccgagct cgccgttgaa ggttgccatt tgtctccgtc gtttagtgcc
     1021 cgtcggttgg gcttgatgat ttgccgattg gatttctcaa atgcctagcg aggagaggcc
     1081 gcagaaaaca agaatggccg caatgaacgc aaagggctca aaaagaatcg gccatacgac
     1141 acacgcttgt ttgcctttgc tgatgccgtc ggttgtttcg gctagaactc ggttacgttt
     1201 ctaaccttga ttttcttgcg cttggcctgg tcgaggacga acttgccgac ggccaggtct
     1261 tcgtcggcga tgccgttgga ttgaaaaaag atgcgcttgg ggcgcggctg cggcgcgccg
     1321 ccggcgacga acttttccag cggcaggatg tcttcccagc gggcgatgcg ggtttggcaa
     1381 gcttcgacta agtcaccgct atcggctctg gcggcggcga tgtcgtcggt gacaatcagg
     1441 tccatgcgcc ggattaggct attggaaact tcgtgcttga ctgtggagtt tgcgccgatt
     1501 gaggcgacca gcacgtcagg cttcaggtta tttcccgtcg ccaccggtgt cgccgaatcc
     1561 gtcgatacca ccaggatgtc cgagagccct tcgcactcgt cgatgctgtc gacttctttc
     1621 caatccactt tcttgaccac tttgctcatt tgcttgacga aatccttgcg ccgcttggga
     1681 gtccggcccc agacgacgat ttgttcgatg tcgcaggctt ccatgacggc ttctatttga
     1741 aaagtcgctt gccagccggg gccgatgatg ccgagaatct tactgtccgc gggagcgaga
     1801 tacttggcgg cgaccccggt agcggcgccg gtgcgcaggc tgctcaaaaa ggccatgttg
     1861 acgatccctt ggatgccgcc cttgccgccg tgaaagagcg tgatgatgtt ggcgccggcg
     1921 gcgtaggagc gcaggcagat cagatcggaa tcctgattcc aggctgccat catgttgagc
     1981 tgtttggagc tgcccttgag acgacggcgc gggacgttgc gcattttacc ggcggcgcgg
     2041 tcgcgaaaca ttttgtcgac caggttgacg gcttgggaca tgtcgatgag tttacgagtc
     2101 tgtgcttcgc tgatgatcac tgccatggct agaaaatcct cccagcgttg tatagcccag
     2161 gaaatctgcc ggggcaagtt atcgtttatg cgattttatc ttggccgacg tctaatcgac
     2221 aaaagccttg acggtgccgg cggatggccg caaaatgatc tggattcaac gtcgcccgaa
     2281 tgaacaaatc gatagcgtcg tcgagagtca gtgtggagta gatgccaatg gcaatatcga
     2341 acatccaaac ccgtctttgt ccgcactgcg cgaatagcat cgcgctcgac acgttgacct
     2401 gcccatattg ccgggcgagt cttatgcccg tgccagtgcc gcagtggccg agccacgata
     2461 aagagtcacc gtcggccgac gttaccccga gaaaatctgc cattccgttg ggttcgaagc
     2521 tgattctggt cttgggcctc gtggttttcg cgctcggggt gtttctcgtc ggcggacaca
     2581 gcgagcggtc aacccttgag ccgatacttg aagttaaaga aaaagcgctg caagcaagcg
     2641 agcaaaaggt taagacgctc gaagcacagt tggtgcaatg ggataaagca ttgagggcca
     2701 gcgagagcca actcgaagag tttaaaacca agcttaacgc ccaggaaaag agtctggcgg
     2761 cggcgcagca aaagcgcaag gacgccgagc gggaagtaaa caggttggca agccgccccg
     2821 cgccagcacc atcgccggtg cgctcgcgcg cgcagaatcc ggctttgccg ccgccaccgc
     2881 caacgccggt ggcgcgccgg atcgtcgagc cggggttgta tgaaaccgtg cgcgccaccg
     2941 acgtgtatga ggagccgctg gccagttcgc gcgtgctcaa ccgggtcggc ggcggcacgg
     3001 aaatcacagt ggtgcgcggc ctcggccagt agttggaagt gcgctcagaa cacggtaatc
     3061 ctcctcgctt tattcgcact gaagatgcta agttcgtccg cggagccaac tagagcacaa
     3121 ccgcgatcct cgaccgttcg cctgatactg cggatctttc cgttttccat ttcgcggctt
     3181 tccgttcgct aacattttcc cgggctctaa cggcgctgat cgattgccag cgcgaccagg
     3241 aacaattgtc ttgaaagagc ctgctaccat ccaatctgac gcgccggtaa tggcgcaggg
     3301 ttttctcgcc aagactttca cctcacttag tcatcgcaat taccgctatt tgtggtttgg
     3361 cacggtgttc atgagcgcgg gtcaatggat ccaacaggtg acgctcggtt ggatagtcta
     3421 cgatctgacc ggctcttcgg ttttactggg tgtgcttaac ggactgcgcg ctctgccgtt
     3481 cctggtcgcc agtccgatcg ccggcgtctt cgccgaccgc atggaccggc gtcagctgat
     3541 acttggctgc gaatgctttc tagccctgtt gactctcctc atgggtgtcg tggtcgtgtc
     3601 cgggaaattg gcggtctggc atttgtttag cttcacgttg ttgaccggtg tcgcttgggc
     3661 attcgtcgat ccgatccgcc agagtctggt gccggtgctg gtgccgaaac cggatctcat
     3721 gaacgctgtg gcgctgaact cggcggcgtt caacatgacc aaagtcatcg gcccttcgct
     3781 cggcggctta ctgattgtcg ccttcggcgc ggcgggcaat tttttcgttc agggagccgc
     3841 ctacgcggtg gtagtattaa tcgtctatcg gatggtcgtt cccgcgacac ccggcgaggc
     3901 gcgacgcagc tctcctttgg cgaatctcaa ggaagggctt agctacgttt ggaccaatcc
     3961 catcgttttc gccctgatgg ccgcagcgtt ggtgccgcgg gtgttcgcca tgccgtttca
     4021 aacgcttatg ccggtatttc aaaaagatgt gctgcaagtg gggccggaag ggctgggcat
     4081 gcttttagcc gcgcctggct tggatgcact gctagccggt ctcgccctgg ctaccctgtc
     4141 gagtcggatc aaacgccagg gcgt
//
LOCUS       DASQKS010000571         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3270615, whole genome shotgun sequence.
ACCESSION   DASQKS010000571 DASQKS010000000
VERSION     DASQKS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270615"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="tal"
                     /locus_tag="VE170_14615"
     CDS             complement(<1..1112)
                     /gene="tal"
                     /locus_tag="VE170_14615"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HYJ16726.1"
                     /translation="MNPLKELSAQGQSIWLDYIRRNLIRSGELKRMVDEDSISGVTSN
                     PTIFEKAIAGSTDYDEALRALLARDGGADVEKLYESLAIEDIQMAADVLRPVYDASNG
                     DDGYVSLEVSPHLAHDTAGTIDAAKRLRAAVDRPNLMVKVPATPAGIPAIEQLIADGL
                     SINVTLMFSMSHYEAVAHAYVKGLERCATPAKVASVASFFVSRVDTAVDNELAKIGTA
                     EAQALAGKIAIANSKVVYRRFMEIFHGEGFASLRSRGARVQRPLWASTGTKNPAYSDV
                     LYVENLIGAETVNTLPLDTIKAFGDHGKVPGATVKSAFDEADAALAALAKLGVDLDAI
                     TEKLQVDGVASFAASFDQLTAALKKKRELMVDVALN"
     gene            complement(1125..1583)
                     /gene="rpiB"
                     /locus_tag="VE170_14620"
     CDS             complement(1125..1583)
                     /gene="rpiB"
                     /locus_tag="VE170_14620"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HYJ16727.1"
                     /translation="MRIVIGADHAGFELKQILAAYLRHQGHEIIDKGTDSTEPVDYPD
                     FAEAVGNAVLAGAAERAVLVCGSGVGASVSANKLPGIRAGLCHDSYSAHQGVEHDDMN
                     ILVLGARIIGVEVARELVDRFLAAKFTGEERHRRRVEKIKALERRYSKTV"
     gene            complement(1586..3637)
                     /gene="tkt"
                     /locus_tag="VE170_14625"
     CDS             complement(1586..3637)
                     /gene="tkt"
                     /locus_tag="VE170_14625"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYJ16728.1"
                     /translation="MNQSVDQLSINTIRTLSMDAVQQANSGHPGTPMSMAPVMYGLWQ
                     RHLKYDPADPLWPNRDRFVLSIGHASMLLYATLFLASVRAVDADQRVLNQPTLTLDDL
                     KKFRQLDSKTPGHPEFGLIAGVETTTGPLGQGVANSVGIAMASKWLASYFNRPGFELF
                     DYSAYALCGDGDMMEGISSEAASLASHLKLDNLCWIYDSNRISIEGSTSLALSEDVGK
                     RFEAYGWNVLHVADANDQAALDGALENFRQCHDRPTFIVIESHIAFGAPTKQDTAAAH
                     GEPLGEGEIKLTKKNYGWPEDEKFFVPEGVQENFQRVIGKRGRALRDEWTGKFAAYQK
                     KYPELAEQLTTMQRRELPAGWDQELSEYPADPKGQATRDSSGKVLNALAKHVPWLMGG
                     SADLAPSTKTRLTFDGAGDFAAGNYAGRNLHFGVREHVMAAIVNGMSLCGVRVFGSGF
                     LIFSDYARPAIRLGAIMEVPAIHVFTHDSIGVGEDGPTHQPVEQLASLRAIPGLITLR
                     PGDANEVVEAWRVIMALKHEPVALALTRQPLPTLDRTKYGAAAGLAKGAYILADADGG
                     KPQVILIGTGSEVYLCVEAYEQLKKDGVQARVVSMPSWELFEHQSQEYREKVLPAAVR
                     ARVAVEQGVAMGWAKYVGMTGAVIGMKSFGASAPFSQLQKKFGFTVANIVAAAKAQLS
                     G"
BASE COUNT          749 a         1210 c         1160 g          819 t
ORIGIN      
        1 tggttcaaag cgacatccac catcagctcg cgcttttttt tcaacgccgc cgtcagctga
       61 tcgaaggaag ctgcgaacga cgccacgccg tcaacttgca acttctcggt gatcgcatcg
      121 aggtcgacgc ccagcttggc caatgccgcc agagcggcgt cggcctcgtc gaaagcgctt
      181 ttgaccgtcg cgccgggaac cttgccgtga tcgccaaaag ctttgatggt atccagaggt
      241 aacgtgttga cggtttccgc gccgatgaga ttctcgacgt aaagcacgtc ggagtaggct
      301 gggttcttgg ttcccgtgct cgcccacagc ggccgctgca cgcgcgcgcc gcggctgcgc
      361 agtgacgcga atccttcgcc gtgaaagatc tccatgaagc ggcgatagac gaccttggag
      421 ttggcgatgg cgattttgcc ggcaagcgct tgcgcttccg ccgtgccgat tttggccaat
      481 tcattgtcca ccgcggtgtc gacccggctg acgaaaaatg acgccaccga ggcgactttg
      541 gccggcgtcg cgcaacgttc caaacctttc acgtaagcgt gcgccaccgc ttcatagtgg
      601 ctcatggaaa acatcaaggt gacgttgatg ctcaggccgt cggcaatcag ctgctcgatg
      661 gcggggatgc cggccggcgt cgccggtact ttgaccatca ggttgggccg gtcgacggcg
      721 gcgcgcaggc gcttggcggc gtcgatcgtc cctgccgtgt catgggccaa atggggcgac
      781 acttcgaggc tgacgtaacc gtcgtcgcca ttgctggcat cgtaaaccgg acgcagtacg
      841 tcggctgcca tctgaatgtc ttcgatggcc agcgactcgt agagtttttc cacgtccgcg
      901 ccgccatccc gtgccaacag ggcgcgcagc gcctcatcgt agtcggtgct gccggcgatg
      961 gctttttcaa aaatcgttgg attgctggtg acgccgctga tgctgtcttc atcgaccatg
     1021 cgcttgagtt cgccgctgcg aatcaagttg cgccgaatgt aatcgagcca gatcgactgt
     1081 ccttgggcgc taagctcttt taatggattc atagttagtc tccgtcaaac cgtcttgctg
     1141 tagcgccgct ccagggcctt gattttctcg accctgcgcc ggtgccgttc ttcgccggtg
     1201 aatttcgccg ctaaaaaccg gtcgaccaac tcacgcgcga cttcgacgcc gatgattctg
     1261 gccccgagca cgagtatgtt catgtcatca tgctcgacgc cctggtgtgc cgaataactg
     1321 tcatggcaca atcccgcgcg gatccccggg agcttgttcg ccgagaccga agcaccgacg
     1381 ccgctgccgc agaccagcac ggcgcgttcc gccgcccccg ccagcacggc attgccgacc
     1441 gcttcggcga agtcgggata atccaccggc tcggtgctat cggtcccttt gtcgatgatt
     1501 tcatggcctt ggtgacgcag gtaggcggcg agaatttgct tcaattcgaa gcccgcgtga
     1561 tcggcgccga tgacaatgcg catgcttacc ctgacaattg cgctttagcc gcggcgacaa
     1621 tgtttgccac ggtaaagccg aattttttct gtaattggga aaacggcgcc gacgcgccga
     1681 aacttttcat accgatcacc gcgccggtca tgccgacata ttttgcccaa cccatggcca
     1741 cgccttgctc gacggcgacc cgcgcgcgca ccgccgcagg cagaaccttt tcgcgatatt
     1801 cctggctctg gtgctcgaac aattcccatg acggcatgct gacgacacgc gcctggacgc
     1861 cgtctttttt gagctgctcg taggcttcga cgcacaaata aacttcgctg ccggtgccga
     1921 tcaaaatcac ctgcggcttg ccgccgtcag cgtcggccaa gatataggcg cccttagcca
     1981 agccggcggc ggcgccgtac ttggttcggt ccaatgtcgg cagcggctgg cgggtcaacg
     2041 ccaaggccac cggctcatgt ttgagcgcca tgatcacacg ccaggcttcg accacttcgt
     2101 tagcatcgcc ggggcgaagc gtaatcagac ccggaatcgc tcggagcgac gccagctgct
     2161 ccaccggctg atgggtcgga ccgtcttcac cgacgccgat ggagtcatgg gtgaagacgt
     2221 gaatcgcggg aacctccata atcgcgccga gccggatcgc cggccgcgca tagtcgctga
     2281 agatgagaaa ccccgaaccg aaaacgcgca cgccgcacag ggacatgccg ttgacgatcg
     2341 ccgccatgac atgctcacgc acaccgaagt gtaaattgcg tccggcgtaa ttgccggcgg
     2401 cgaaatcgcc ggcgccgtcg aaggtgaggc gcgtcttggt cgagggcgct aggtcggcgg
     2461 aaccgcccat cagccaaggc acatgcttgg cgagagcgtt caaaactttg ccggaggaat
     2521 cacgcgtcgc ctggcctttt ggatcggccg gatattcgct caactcctgg tcccagcccg
     2581 ccggcagttc gcgccgctgc atggtcgtca attgctcggc gagttccggg tattttttct
     2641 ggtatgcggc aaatttgccg gtccactcat cgcgcagcgc gcggccgcgt ttgccgataa
     2701 cccgttggaa attctcctgc accccctcgg ggacgaaaaa tttctcatcc tccggccagc
     2761 catagttttt cttggtcagt ttgatctcgc cctcgcccag cggctcgcca tgagcggcgg
     2821 cggtgtcctg cttggtcggc gcgccgaagg cgatatggct ctcgatcacg ataaacgtcg
     2881 gtcggtcgtg acattggcgg aagttttcca gcgccccgtc gagggccgct tggtcgttgg
     2941 cgtcggcgac atgtaaaaca ttccagccgt aggcctcgaa acgtttgccg acatcctccg
     3001 acagcgccag actggtgctg ccctcgatgg agatccggtt gctgtcgtaa atccagcaaa
     3061 gattgtccag tttcagatgg ctggcgaggg acgccgcttc gctgctgatc ccttccatca
     3121 tatcgccgtc gccgcatagt gcgtaggcag agtaatcgaa cagctcgaag ccggggcggt
     3181 taaagtagct ggccagccat tttgacgcca tcgctatacc gacgctgtta gccacgcctt
     3241 ggccgagggg cccggtggtg gtctcgacgc cggcaatgag accgaactcc ggatggccgg
     3301 gtgtcttgct gtcgagttgg cgaaattttt ttagatcatc gagggtcagg gtcggttgat
     3361 tgagcacccg ctggtcggcg tccacagcgc gcacgctggc caagaacaac gtcgcataga
     3421 gcagcatcga cgcatggccg atggacagca cgaagcggtc gcgattgggc cacagcggat
     3481 cggcggggtc atattttaaa tggcgttgcc acaggccgta catcaccggc gccatcgaca
     3541 tcggcgtgcc gggatggccc gagttcgcct gctgcaccgc gtccatggac aacgtacgga
     3601 tggtattgat ggacaattga tcgaccgact gattcatagg cttgcctgtt ctaaccggag
     3661 cctgcagcta cggttggtta caagtataac tttatcactt tcacgtaccg aggtcagcag
     3721 aataggcgaa ctaatcggca attcttcaag caaatttaat cgcggcgtca atccattggc
     3781 ggcggcgcaa agcctaactt ttccaagcac gccactgtcc gccgcagtgc tgcgcgcaac
     3841 tccgacgccg gcaattgcca caatcgttga aagtcatcgg ccacgtcgac atcgaaccaa
     3901 gtcggcagca cgtcgtaggg aaagttaagc gcctcgag
//
LOCUS       DASQKS010000572         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3685297, whole genome shotgun sequence.
ACCESSION   DASQKS010000572 DASQKS010000000
VERSION     DASQKS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3685297"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2603
                     /gene="rpoB"
                     /locus_tag="VE170_14630"
     CDS             <1..2603
                     /gene="rpoB"
                     /locus_tag="VE170_14630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYJ16729.1"
                     /translation="NYKPVSAALKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGG
                     LTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLIASLTTYARVNDFGFIETPYREVE
                     SGRVSDRIRYLSALEEEDHVIAQANAPIDGRGNFSADLVSSRKGGEFVMARPEEVNFM
                     DVSPNQLVSVAASLIPFLENDDANRALMGSNMQRQAVPLLRTEAPFVGTGMERTVARD
                     SGVTIVARRDGLVESVDSTRIVVKADKPGGSRDTGVDIYNLVKYQRSNQNTCNNQRPI
                     VVVGDQVRAGDVIADGPSTEMGELALGRNVVVALMPWGGYNFEDSILINERVVKEDIY
                     TSIHIEEFECVSRDTKLGPEEITRDIPNVGDEALTDLDESGIIRIGAEVKPGHILVGK
                     ITPKGETQLSPEEKLLRAIFGEKAGEVRDTSLRVPPGVEGTVINVRIFSRKGLGKDER
                     SRSIEDEEATRLRKDQHDEVRILQEGALRRLKKLLVGRRLASRISDDSRKVLLNKGQE
                     VTEEDLDQLPMSYWSEIQVDNESVESELGRIIESTTTQVNLIKHAFEDKIEKLKGGDE
                     LPPGVIKMVKVFVAIKRKLQVGDKMAGRHGNKGVISRILPEEDMPYLEDGTPVDVVLN
                     PLGVPSRMNVGQILEAHLGWAARSLGHQIGDMIRAQSGDIDPLRRRLKDYLNTRDANE
                     LIDGAKDAEVLKLAAKYRDGVRIASPVFDGAAEEEIFNLIRKAGLPASGQATLFDGRT
                     GEAFDGKVTVGVMYIMKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEV
                     WALEAYGAAYTLQEMLTVKSDDVVGRTRMYEAIVKGDNLLEPGLPESFNVMVKELQSL
                     GLDVDLVEEK"
     gene            2651..>3670
                     /locus_tag="VE170_14635"
     CDS             2651..>3670
                     /locus_tag="VE170_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYJ16730.1"
                     /translation="MEDLFNLFEKPKNPLSFNAIKVSVASPEKIRSWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPTKDYECNCGKYKRMRHRGVVCEKCGVEVIQSKVRRERM
                     GHIELATPVVHIWFLKSLPSRIGALLDMSLKDIEKVLYFESYIVVDPGETPLQYKELL
                     TEARYRKAVEEYGGKFVAGMGAEAVRKLLKEIDIPKVADELRQEMRDANSEVRRKKMA
                     KRLKVLTAFRTSGNRPEWMVMEVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELSAPDMIIRNEKRMLQEAVDALFDNGRRGRAITGQNRRPLKSLSEMLKGK
                     TGRFRQ"
BASE COUNT          842 a          978 c         1094 g          756 t
ORIGIN      
        1 tcaattacaa gccggtgtca gcggcgctca aagagttctt tggctccagt cagctgtcgc
       61 agttcatgga tcagaccaat ccgctgtcgg aaatcaccca taagcggcgg ctctcggccc
      121 tcggtcctgg cggtctaacc cgggagcgtg ccggtttcga agtgcgtgac gtgcatccga
      181 cccattacgg ccgggtctgt ccgatcgaaa cccctgaagg tccgaacatc gggctgatcg
      241 cttcgctgac cacctatgcg cgggtcaacg attttggttt tatcgaaacg ccctatcgcg
      301 aagtcgaaag tggccgggtt agtgatcgca tccgttatct atcggccctc gaagaagaag
      361 accatgtcat cgctcaggcc aatgcgccga tcgacggtcg cggcaatttt tccgcggact
      421 tggtctcttc gcgtaagggc ggtgagtttg tcatggcccg tccggaagaa gtcaacttca
      481 tggacgtgtc gccgaaccag ttggtcagcg tagcggcgtc gctcattccg ttcttggaaa
      541 acgacgacgc taaccgcgct ttgatgggct ccaacatgca gcgccaagcc gtgccgctgt
      601 tacgcacgga agcgccgttt gtcggtaccg gtatggaacg cactgtggcg cgcgattccg
      661 gcgtgaccat cgtcgcgcgc cgcgacggct tggtcgaaag cgtcgactcg acgcgtatcg
      721 tcgtcaaagc cgataagcca ggcgggtcgc gtgataccgg cgtcgatatt tacaacctgg
      781 tcaagtacca gcgttccaat caaaatacct gtaacaacca gcggccgatc gttgtggtcg
      841 gcgaccaggt cagagccggc gatgtcatcg ccgacggtcc gtctactgag atgggcgagc
      901 tagcgctggg gcgcaacgtc gtcgtcgcgc tgatgccctg gggcggttac aacttcgaag
      961 attcgattct catcaatgaa cgcgtggtca aagaggatat ttatacctcg atccacatcg
     1021 aagagtttga gtgcgtttcg cgcgacacga agttgggccc cgaggaaatt acccgggata
     1081 ttcctaatgt cggtgacgaa gcgctcaccg atctcgacga gagcggcatt atacgcatcg
     1141 gcgccgaagt taaaccgggc catattctgg tcggcaagat tacccccaag ggggaaaccc
     1201 agttgtcgcc ggaggaaaaa ctgttgcggg cgatcttcgg agagaaggcg ggcgaagtcc
     1261 gcgatacctc cctacgggtg ccccccggcg tcgaaggcac ggtcatcaat gtgcggattt
     1321 tttcgcgcaa aggattgggc aaggatgagc gcagccggtc aattgaggat gaagaggcta
     1381 cccggttacg taaagaccag catgacgaag tgcgcattct ccaagaaggt gcactccgac
     1441 ggctaaaaaa actcctggtt ggccggcgct tggcgagccg gatcagtgac gatagccgca
     1501 aggtcctgct caacaaagga caggaggtga ccgaggagga tcttgatcag ctgccgatgt
     1561 cctactggag tgagatccaa gtcgataatg agtccgtgga gagcgaactc ggccggatca
     1621 tcgaaagcac gactacccag gttaacctga tcaagcatgc gttcgaagac aagatcgaaa
     1681 aactcaaagg cggcgacgag ttgccccccg gggtcatcaa gatggtcaaa gtctttgtcg
     1741 ccatcaagcg taagttgcaa gtgggcgata aaatggccgg ccggcacggt aataagggcg
     1801 tcatttcccg catcctgccc gaagaggaca tgccttattt agaggacggt actcccgtcg
     1861 atgtggtgtt gaatcctttg ggtgtgccat cacgcatgaa cgtcggccag atcctcgagg
     1921 ctcatttggg ctgggcagcg cgcagcttgg ggcaccaaat cggggatatg atacgcgcgc
     1981 agagcggcga tatcgatcct ctgcggcggc gtttgaaaga ttatttgaat acccgcgatg
     2041 ccaatgagtt gatcgacggt gcgaaagacg ccgaggtttt gaagctcgcc gcaaaatatc
     2101 gcgatggcgt gcgcatcgcg tcgccggtat ttgacggggc cgcggaagag gaaattttta
     2161 acttgatacg caaggccggt ttaccggcca gcggccaagc aaccttgttt gatggccgca
     2221 cgggtgaagc cttcgacggc aaagtaactg tcggcgtcat gtacatcatg aagctgcatc
     2281 atttggtcga cgacaagatt catgcgcggt cgaccggacc ttactctctc gttacccagc
     2341 aacccttggg cggtaaggcc cagtttggcg gccagcgttt gggcgagatg gaggtctggg
     2401 cgcttgaggc gtacggcgcc gcctatacgt tgcaagagat gctcacggtc aagtccgacg
     2461 atgtcgtcgg gcgtacccgc atgtacgagg cgatcgtcaa aggcgacaac ttgctcgaac
     2521 cgggtttgcc cgagtcgttt aacgttatgg tgaaagagct ccaaagccta ggcttggatg
     2581 tcgatctcgt tgaagaaaag taggtatccc caagtacggt cgtgcagctc acaccggagg
     2641 tgatcctgat atggaagatc tatttaattt atttgaaaaa ccgaaaaatc cgctcagttt
     2701 taacgcgatc aaagtttcgg tcgcatcgcc ggaaaagatt cgttcttggt cccatggcga
     2761 agtaaaaaag ccggaaacca ttaactatcg gaccttcaag ccggagcgcg atgggctatt
     2821 ttgcgccaag atttttggtc cgaccaagga ttacgaatgc aactgcggca agtataaacg
     2881 catgcgccat cgcggcgtgg tctgcgaaaa atgcggtgtc gaagtcattc agtccaaagt
     2941 gcgccgcgag cgcatgggcc atatcgaact ggccacgcca gtcgttcata tctggttttt
     3001 gaaaagcctg ccgagccgca tcggcgcgct gctcgacatg tcgctcaaag acatcgagaa
     3061 ggtgctctac ttcgagtcct atatcgttgt cgacccgggc gagacgccgc tgcaatacaa
     3121 ggaactcttg accgaggcgc gctatcgcaa agcggttgag gaatatggtg gcaagtttgt
     3181 cgcgggaatg ggcgccgagg cggtgcgtaa gctgctcaaa gagatcgata tccccaaggt
     3241 cgccgacgaa ttgcgccaag agatgcgcga cgctaattct gaagtgcggc gcaagaaaat
     3301 ggccaagcgg ctgaaagttt tgaccgcatt caggacctcg ggcaaccgtc cagagtggat
     3361 ggtgatggaa gtaattccgg tgattccacc ggatcttcgt cccctggtgc ccctcgacgg
     3421 tggccgtttt gcgacctcgg atttgaacga tctctatcgc cgcgtcatca atcgcaacaa
     3481 ccgcctcaaa cggctgatgg aattgagcgc gccggacatg atcatccgca atgaaaagcg
     3541 catgctgcaa gaagcggtgg atgccttatt cgacaacggc cgtcgcggcc gggcgattac
     3601 cggccaaaac cgccggccgc tgaaatcatt gagcgagatg ctcaaaggca aaaccggccg
     3661 cttccgccag
//
LOCUS       DASQKS010000573         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_744922, whole genome shotgun sequence.
ACCESSION   DASQKS010000573 DASQKS010000000
VERSION     DASQKS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744922"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..823)
                     /gene="ccsA"
                     /locus_tag="VE170_14640"
     CDS             complement(116..823)
                     /gene="ccsA"
                     /locus_tag="VE170_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYJ16731.1"
                     /translation="MAQPTTAVKKRKGILGGAALLAIVAGLCWSLFIAPPDGYQGEVQ
                     RIMYLHLPSILTAYLSYFLVFIGSGMYLWKREKKDDLLAHAAAELGVLFTALTIIEGS
                     IWGKPTWGVWWTWDARLTLTAILLLIYAGYLMLRSLIDDEQRAASAAAILGIIGFLDI
                     PLIHMSVYWWRTLHQPPSILRPDKKPWETVHPAMLTALAVSFVGFLLLYFYLLSLRYR
                     VGEMRQEIKALRLERGA"
     gene            complement(826..1503)
                     /locus_tag="VE170_14645"
     CDS             complement(826..1503)
                     /locus_tag="VE170_14645"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HYJ16732.1"
                     /translation="MTLLGQTYLLLSKDLLLELRRRDSVLTMFFFGTLLLFVFHFAFD
                     LPPDKIAELAPALLWLAFLFTGTLGLAQLFQSERDNHCLDALLLSPLDRGALFLAKTG
                     FNFVLMFVVELVVIPLFWVLFNLSAWNLLPRLFLVTLLGTLGFCILGTITAAITLRAR
                     ARELLLPLVLFPLMIPVILATIRCMESIMRTGAMDEAMPWLRLLIGFDVIFLTIGILI
                     FDWVIES"
     gene            complement(1500..2207)
                     /gene="ccmA"
                     /locus_tag="VE170_14650"
     CDS             complement(1500..2207)
                     /gene="ccmA"
                     /locus_tag="VE170_14650"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HYJ16733.1"
                     /translation="MRLIVNQLAKAYGFLWALRPLDLEIVPGEFIALLGPNGAGKTTL
                     LKLLAGLTMPTAGTIELDGKTLAGNSATLRGQIGLLAPADHLYDQLTVLENLRFFAAL
                     YGKAQPKNSLAAALEEVGLAARADAFAGTLSAGMKCRLSIAKWRLLDPALLLLDEPYG
                     VLDGNGVDLLEEFLRQQCAKGHIVIMASHHVSRAVALCSRALVLHQGRLSFNQAKQSP
                     WPSFELAYGDFLPRGGL"
     gene            2404..3078
                     /locus_tag="VE170_14655"
     CDS             2404..3078
                     /locus_tag="VE170_14655"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16734.1"
                     /translation="MNPVVTVTGFWDAFVTKIAVFLPNLLFAVAIILLGWVVCNVIKR
                     VVVRLLKLCQFDTLAERSGIKRILERGGVRQTPSDIVGLLVFWFLFLIAIVTTLETLN
                     LSGATDTLHTIYLYIPKIVAALVTLILGLYFANFLESVTRTSCANAGLEAAESIGRAT
                     YIGTTIFVVAAIFEILDIASEIVIWAFILVFGAACLALALAFGLGGREVAGRFLEKWL
                     EQKKNE"
     gene            3207..>4191
                     /locus_tag="VE170_14660"
     CDS             3207..>4191
                     /locus_tag="VE170_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYJ16735.1"
                     /translation="MADYKVVKVPAGGAKITMGSNKKLVVPDNPVIPFIEGDGTGRDI
                     WRASVRVFDAAVAKAYGGKKKIHWMEVYAGEKSFKQYNSWLHEETVPAFSEFLVGIKG
                     PLTTPIGGGMTSLNVGLRKLLDLYVCLRPVRYFKGVPSPVKHPEFVDMVVFRENTEDI
                     YTGIEFPNGTEANAKFKSLVKETFPKEFAKIRFPNSASIGLKPISVEGTERLVRAAIQ
                     WALVNKRKNVNFVHKGNIMKYTEGSFMEWGYALAKREFRNDVVAERESWILGNKEANP
                     NLSVADNAKAIDPGFEMMSPDQQNDIKKEVEDALKLWPTHGDGKWKSKLMLK"
BASE COUNT         1033 a         1102 c         1179 g          877 t
ORIGIN      
        1 aaccggttta gctccgcttc ggcctgacga taacggcgct tcaagaataa ccaatacagg
       61 acgatgacgg cgcaaacaat tccataagct aatgaaacaa atccccaggg tcccatcaag
      121 cacctcgctc caggcgcaat gctttgatct cctggcgcat ctcgccgacc cggtagcgca
      181 gcgaaagtag atagaaatag agcaggagaa agccgacaaa gctgacggcc agggcggtaa
      241 gcatggcggg gtggacggtt tcccatggct ttttgtccgg ccggaggatc gacggcggct
      301 ggtgcagtgt gcgccaccag tagaccgaca tatgaattag cgggatatcg aggaatccga
      361 tgatgcccaa aatcgccgct gccgaggcgg cgcgctgctc gtcgtcgatc agcgaacgga
      421 gcatcaaata accggcgtaa atcagcagca ggattgccgt caacgtgagc cgggcgtccc
      481 aagtccacca cacaccccag gtcggtttgc cccagatcga gccttcgata atggttagcg
      541 cggtaaacag gacgcctaat tcggcggcgg catgggcgag caggtcgtct ttcttctcgc
      601 gtttccacag atacatgccg ctgccgatga acacgagaaa ataggataga taggcggtca
      661 aaatgcttgg cagatgaaga tacatgatgc gctgcacttc accctgataa ccgtccggcg
      721 gtgctatgaa caggctccag caaagtccgg ctacaatggc tagcaaggcc gcgccgccga
      781 ggatgccctt gcgcttctta accgctgtcg tgggttgcgc catgattagc tttcgatcac
      841 ccaatcaaaa atcaagatcc cgatggtcaa aaaaattaca tcgaagccga tcagcagccg
      901 aagccatggc atggcttcgt ccatggcgcc ggttctcatg atgctttcca tgcaacgaat
      961 ggtggcgagg attaccggaa tcatcaaggg aaacaagacc aggggcaaaa gcagctcgcg
     1021 cgctctagcg cgcaaagtaa tggcggcggt gatcgtgcct aaaatgcaaa agcccagggt
     1081 gcccaataaa gttaccaaaa agagtcgcgg tagcaagttc catgcggaca ggttgaacaa
     1141 aacccagaac aacgggatga ccaccagttc gacgacaaac attagcacga aattgaaacc
     1201 ggtcttggcg agaaacaacg cgccgcggtc gagcggcgac aacagcaatg cgtcgaggca
     1261 atgattatcc cgctctgatt gaaacagctg cgccaagccg agggttccgg taaacaaaaa
     1321 tgccaaccac agaagagccg gcgccaattc ggcgatcttg tccggcggca aatcgaaggc
     1381 gaagtgaaag acgaataaca gcaaggtgcc aaaaaaaaac atggtcaaca cgctgtcgcg
     1441 ccggcgcaac tccagcaata agtccttgct gaggagcaag taggtttgtc cgagcagggt
     1501 cataagccgc cacggggtag gaagtcgcca taggcgagct cgaaactggg ccagggcgac
     1561 tgcttggctt ggttaaaact caagcgtccc tgatgcagga ccagtgcgcg gctgcacaga
     1621 gccaccgccc gtgacacatg gtgcgaagcc atgatgacga tatgcccctt ggcgcattgc
     1681 tggcgtagga attcttccaa caaatcgaca ccattgccat cgaggacgcc gtagggttcg
     1741 tcgagaagga gcaaggcggg gtcgagcagg cgccatttgg caatagatag gcggcatttc
     1801 atgccggccg aaagggtgcc ggcgaatgca tcggcgcgcg ccgccaggcc gacttcttcc
     1861 aatgccgccg ccaagctatt ttttggctga gctttgccat agagcgcggc gaagaaccgc
     1921 aagttttcta aaaccgtaag ttgatcgtag agatggtcgg caggggcgag tagaccgatt
     1981 tggccgcgca gagtggcgga gttcccagcc aaggtcttgc cgtcgagctc gatggttccg
     2041 gccgtaggca tggtcaggcc ggcgaggagt ttcaatagcg tcgttttgcc cgcaccgttg
     2101 gggccgagca gggcaatgaa ctcgccaggc acgatttcga gatcgagggg ccgcagcgcc
     2161 cacaagaagc cgtaggcttt ggcgagttga ttaacgatta ggcgcaaggg tcgatcccaa
     2221 ttttcaatct gcgaaagccg gcgagaatca cttcgctgcc cgggcggctc gactatagtg
     2281 accgtcagtg cccgattcaa gcagcgatct aatattgttt cttttggcca tttggttgac
     2341 tttttaaggc tgagacctct atgtaaatag gcaattcaat acgctgtgaa gtaggaggaa
     2401 tcaatgaatc ccgtggttac ggtcaccggg ttttgggacg cattcgttac gaagatagca
     2461 gtctttctac ccaacttgct gttcgccgtg gcgattatat tgctcggttg ggtggtttgt
     2521 aatgtgatta aacgggtggt cgtccggctg ctgaaacttt gccaattcga tacactcgcc
     2581 gaacgctccg gcatcaagag gatcctcgaa cggggcggcg tgcgccagac gccatcggac
     2641 atcgttggcc tattggtgtt ctggtttctc ttcctcatcg ccatcgtcac cacgctggaa
     2701 acgctaaatt tgagcggcgc caccgatacc ctccatacca tctatttata tattcccaag
     2761 atcgtcgccg ccctggtgac gctgattttg ggactctact tcgccaattt tctcgagtcg
     2821 gtgacgcgga cttcttgcgc caatgcgggt ttggaagccg ctgaatccat tggccgggcg
     2881 acctatatcg gtacgacgat ttttgtcgtc gccgccattt tcgaaattct cgatatcgcc
     2941 tccgagatcg tcatttgggc attcatcttg gtgtttggcg ccgcctgtct ggcactcgcc
     3001 ttagcctttg gcttaggtgg ccgagaagtc gccggccggt tccttgaaaa gtggttggag
     3061 cagaagaaaa acgagtagtt agcaaccttg actcgcagta gctaatcagc tagtttcgaa
     3121 tcgttcaaaa tatattttta cgcaaatagc ctaaagcgcg cggaatcgct cgacgctctt
     3181 aggctttttt tgcacgtgag gagattatgg cagattacaa agtggtaaaa gttcccgctg
     3241 gcggcgcgaa gatcaccatg gggtccaaca agaaacttgt ggtcccggat aatccggtca
     3301 taccgtttat cgaaggcgat ggcacgggac gcgatatctg gcgcgcttcg gtgcgggttt
     3361 tcgacgcggc ggtggccaag gcttacggcg gcaagaagaa gatccattgg atggaagtct
     3421 atgccggtga aaaatccttc aagcaatata atagctggct gcacgaagaa acggtgccgg
     3481 catttagcga atttctcgtt ggcatcaagg gccccctgac cacaccgatc ggcggcggca
     3541 tgacgtcgtt gaacgtcggc ctgcgaaagt tgctcgacct ctacgtatgc ctgcgacccg
     3601 tgcgttattt caaaggtgtg ccttcgccgg tgaagcatcc tgaatttgtc gacatggtgg
     3661 ttttccgcga aaacaccgaa gacatttaca ccggcatcga gtttcccaac ggcaccgagg
     3721 ccaacgccaa atttaaatcg ttagttaaag aaaccttccc caaggaattt gctaaaattc
     3781 gctttcctaa cagtgcctcc atcggcctaa aacccatttc cgtggaaggc accgagcgct
     3841 tggtgcgcgc ggcgattcag tgggcgctgg tgaacaagcg taaaaacgtt aactttgtcc
     3901 ataagggcaa catcatgaaa tacactgagg gctccttcat ggaatggggc tacgccctcg
     3961 ccaagcgcga atttcgtaac gacgtggtcg ccgagcgcga atcgtggatc ctcggaaaca
     4021 aggaagccaa ccctaacctc agcgttgccg ataatgccaa agcgatcgat cccggtttcg
     4081 aaatgatgtc gccggatcaa cagaacgata ttaaaaaaga agtcgaagac gctctcaagc
     4141 tgtggccaac ccatggcgac ggcaagtgga agtccaagct tatgcttaaa g
//
LOCUS       DASQKS010000574         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_285934, whole genome shotgun sequence.
ACCESSION   DASQKS010000574 DASQKS010000000
VERSION     DASQKS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285934"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="sufD"
                     /locus_tag="VE170_14665"
     CDS             <1..743
                     /gene="sufD"
                     /locus_tag="VE170_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HYJ16736.1"
                     /translation="LLGAGSEAKIVESYLGVSGKAYFCNAVTELVGAEDSIVEHYRLQ
                     QDSELGFHVGTLAASLARNCHMTAHAVTLSGALVRNNVRVLLNGAGAACVLNGLYLAD
                     GKQHVDNYTLIEHVSPRASSLELYKGVLGGAAHGVFNGKIIVHKDAQKSDARQTNKNL
                     LLSDSAVIDTKPQLEIHADDVKCSHGSTIGQLDADAIFYLRSRGLDVAAARSLLSFAF
                     ASDVVRRLGIEHLRQRLDAYLVGRFGAW"
     gene            760..2013
                     /locus_tag="VE170_14670"
     CDS             760..2013
                     /locus_tag="VE170_14670"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HYJ16737.1"
                     /translation="MSHKTDGVVASEKFDVARVRQDFPILSRKIHGKPLVYLDNGATS
                     QKPQCVIDAIDHYYRAENSNIHRGVHHLSEQATAAYEAARGKLRGFINAGSDRQIIFV
                     RGTTEAINLVAQSYGRSFLKAGDEIIVSAMEHHSNIVPWQMLCEQVGAKLRVIPINHD
                     GEIVMEEYQRLLNDRTKFVSVTHVSNALGTVVPVKEIIALAHERGVPVMLDGAQAVPH
                     LKVDVQDLDCDFYAFSGHKMFGPTGVGVLYGRTELLEKMPPYQGGGDMISLVTFEKTH
                     YNVLPYKFEAGTPNIADGIALGAAVDYLRALDWPAVVAHEDALLHYATEALGQIPALR
                     IIGTAKVKAGVLSFIFDHVHAHDVGTILDQEGVAVRAGHHCAMPVMQRFGVPATARAS
                     FALYNTTEEIDVLVRAIERVLKVFG"
     gene            2013..2465
                     /locus_tag="VE170_14675"
     CDS             2013..2465
                     /locus_tag="VE170_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HYJ16738.1"
                     /translation="MSELDDLYQEVILDHNKSPRNFRVMSAANRRAEGYNPLCGDQVT
                     VFLQVEEGVIKDISFQGSGCAISKASASMMTTELKGKPESAAYELFDNVHHMLTGESC
                     DASGAERLGKLAVLSGVCKFPARVKCASLAWHTANAALKGEKQPAKTE"
     gene            2499..>3012
                     /locus_tag="VE170_14680"
     CDS             2499..>3012
                     /locus_tag="VE170_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYJ16739.1"
                     /translation="MVTDQPIILKRDCAAVMVPSGEPLALHAGGKVWLTQALGGGYTV
                     MTDHGYTVRIDGRDGDALGLSDGQSPTVVETPTASGSVQERVWEQLRRCFDPEIPVNI
                     VDLGLIYDCQVEPLPGGGHKATIHFTLTAQGCGMGEFLKADIKRKLLEVTDVREAAVE
                     LVWEPQWNQSR"
BASE COUNT          621 a          848 c          932 g          611 t
ORIGIN      
        1 ttttgctcgg cgccggcagc gaggcgaaga tcgtcgaaag ttatttaggc gtgagcggca
       61 aggcatattt ttgcaacgcc gtcaccgaat tggtcggggc tgaggactcc atcgtcgagc
      121 actaccgctt gcagcaggac agcgaactcg ggtttcatgt cggcacgttg gcggcgagct
      181 tggcgcggaa ctgccatatg accgcccacg cagtgactct gagcggcgcg ctggtgcgta
      241 acaatgttcg cgtgctgttg aacggcgcgg gcgcggcgtg cgtgctcaat ggactttacc
      301 tcgccgacgg caaacagcat gtcgacaatt acactttaat cgagcatgtc agcccacggg
      361 cgtcgagcct ggaattgtac aaaggggtgc tcggcggcgc agcccacgga gtcttcaacg
      421 gcaaaatcat cgtccacaaa gacgcccaga agtccgacgc gcgccagacc aacaagaatt
      481 tattattgtc ggatagcgcg gtcatcgaca ccaaaccgca attggaaatt catgccgacg
      541 atgtcaaatg cagccatggc tcgaccatcg gccagctcga tgccgatgcg atattttatc
      601 tgcgctcgcg cggcttggat gtggcggcgg cgcgcagctt gttgagcttt gcctttgcca
      661 gcgacgtggt gcgccggctg ggtatcgaac acttgcgcca gcgtttggac gcgtacttgg
      721 tcgggcgatt cggagcgtgg tgaagttcct atgaaggcaa tgtcgcacaa aaccgatggg
      781 gtcgtggctt cagagaaatt tgacgtcgcc cgggtgcgcc aggactttcc catcctgagc
      841 cgtaagattc acggcaagcc gctggtctat ctcgacaacg gcgccaccag ccaaaagccg
      901 cagtgcgtga ttgacgctat cgatcactac taccgtgcgg aaaattccaa catccaccgc
      961 ggtgtgcatc acttgagcga gcaggccacg gcggcctatg aagccgcccg cggcaaactg
     1021 cgcggtttta tcaacgccgg gtcggatcga cagattatct tcgtgcgcgg caccaccgag
     1081 gcgatcaatt tggtggcgca gagctacggc cggtcgttct tgaaagcggg cgacgagatt
     1141 atcgtctcgg ccatggaaca tcactccaac atcgtgccgt ggcaaatgtt gtgcgaacag
     1201 gtcggcgcca agctgcgggt gattccgatc aaccatgacg gcgagatcgt catggaagaa
     1261 tatcaacgct tgctcaacga ccgaactaaa tttgtctcgg tgactcatgt ttccaatgcg
     1321 cttggcaccg tggtgccggt gaaggagatt atcgccctgg cccatgagcg cggcgtgccg
     1381 gtgatgctcg acggcgctca agcggtgccg cacttaaaag tcgacgtgca ggatctcgat
     1441 tgcgatttct acgccttctc cggtcataag atgtttggcc cgacgggggt cggagtattg
     1501 tacggccgta ctgaactgct ggaaaaaatg ccgccgtatc agggcggcgg tgacatgatc
     1561 agtctcgtga cttttgaaaa aacccattac aacgtcctgc cctataagtt cgaggcgggc
     1621 acgccgaata tcgccgatgg catcgccttg ggcgcggctg tcgactattt gcgcgccctc
     1681 gattggccgg cggttgtcgc ccatgaggac gcattgctgc attacgccac cgaggcgttg
     1741 ggacaaatcc ccgcactgcg cattatcggc actgccaagg taaaggccgg ggtgctgtca
     1801 tttattttcg accatgtgca tgcccatgac gtgggcacga tcctcgatca ggaaggcgtc
     1861 gcggtgcgcg ctggccatca ttgtgccatg ccggtgatgc agcgtttcgg cgtgccggcg
     1921 acggcccggg cgtccttcgc gctttataat accaccgaag aaattgacgt gctggtgcgc
     1981 gccatcgagc gcgttttgaa ggtgtttggc taatgtcgga actcgacgat ctctatcaag
     2041 aagtcatcct cgaccacaac aagagtccga ggaactttcg cgtcatgagc gcggccaatc
     2101 gccgagcaga gggttacaat ccgctgtgcg gcgatcaggt gacggtgttt cttcaggttg
     2161 aagagggcgt tatcaaagac atcagcttcc aaggctcggg ctgcgccatc tccaaggctt
     2221 cggcgtcgat gatgaccact gagctgaagg gcaagcccga aagcgcagcc tacgaacttt
     2281 ttgacaatgt ccatcacatg ctgaccggcg agtcctgtga tgccagcggc gccgagcggt
     2341 tgggtaagct cgcggtgctt tccggtgtat gcaaattccc cgcgcgggtg aaatgcgcca
     2401 gtctggcctg gcacaccgcg aacgcggcgc tcaaaggcga aaagcagccg gcaaagaccg
     2461 agtgattcgg tcactcgcaa ccaaacgagg ggatcccgat ggtaactgac caaccgatca
     2521 ttcttaaacg ggactgcgcg gcggtgatgg tgccaagcgg cgaaccgctg gcgcttcacg
     2581 ccggcggcaa ggtctggctc acccaagcgc tgggcggcgg ctacaccgtc atgaccgacc
     2641 acggttatac ggtgcgcatc gatggccgcg atggcgatgc ccttggttta tccgacggcc
     2701 agtcgccaac ggtggtggaa acgccgacgg cttcgggctc ggtgcaggag cgcgtgtggg
     2761 agcaattgcg ccgttgtttc gatccggaaa ttcccgtcaa tatcgtcgat ctgggactga
     2821 tctatgactg ccaggtcgag ccccttcccg gcggcggcca taaagcgacg atccatttta
     2881 ctttgaccgc ccagggctgc ggcatgggcg agtttctcaa agccgatatc aagcgcaagt
     2941 tgctcgaagt gacggatgtg cgcgaggcgg cggtcgagct ggtctgggag ccgcagtgga
     3001 accagagccg ca
//
LOCUS       DASQKS010000575         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5362459, whole genome shotgun sequence.
ACCESSION   DASQKS010000575 DASQKS010000000
VERSION     DASQKS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5362459"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..436)
                     /locus_tag="VE170_14685"
     CDS             complement(311..436)
                     /locus_tag="VE170_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16740.1"
                     /translation="MASKKADFRTFYRDAHAVECLSEREKMLMGLAVAMMRLCDP"
     gene            complement(565..1626)
                     /locus_tag="VE170_14690"
     CDS             complement(565..1626)
                     /locus_tag="VE170_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ16741.1"
                     /translation="MADEHKHEHKGEHSHDHGEEHHDDKHGHSHSHEAHGHEHGHEAH
                     GHEHAHGSDHGHEQEHGHGHDHDHGEKHDHAQQGHSHGHGDSHSHDHKSRFKDPAHAA
                     EFDQRGLSDMRGQLTAKLIEALTLKGGEAILDLATGTGRVARPLSKQMQSGRIVGVDE
                     ALAMLDVGHHHKDPIARYELSAGEADKLPFKSGTFDRAFVSFSLHHFGNPAGVVGEVQ
                     RVLKPSGRFVVLDPVMEESKDAIDVALEAKINQVFRRTHGEGFRFHTVSSIQRLLTKA
                     GYRVPRTSVLSYPFNQEGMDGIPTGRHWLEAALELEKEAPELAERMKKNYFTWHSHGD
                     HAHVKGSFSYALITGVKPA"
     gene            complement(1658..2071)
                     /locus_tag="VE170_14695"
     CDS             complement(1658..2071)
                     /locus_tag="VE170_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HYJ16742.1"
                     /translation="MATRWLFKTEPSVYSYQQLVKDKSTMWDGVANNLALMHLKDIKK
                     GDQIFIYHTGDEKQAVGIARALGGAYSDPGKDNPRMLVVDIEAVKALDKPVTLAEVKA
                     DKKLANFDLVRNSRLSIMKVTDEQWDAMVEMSKKK"
     gene            complement(2322..3350)
                     /locus_tag="VE170_14700"
     CDS             complement(2322..3350)
                     /locus_tag="VE170_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16743.1"
                     /translation="MQLSGEASMRSICCFMFLCAAVFFSAPSGRAQSIDETYKQALKE
                     GGVLNVYGTLTPDTAEKVLPIFEKRFPGIKTVNTGASSDKIVARAISEARGGRTIGDV
                     FHMNLENIMQVHEQGLVLEKLPPEADVFAGHLKGTYWVATRFNTLVAAWNTQLVGKAE
                     EPKSFEEFAEPKWKGKLLAEPRDAEFLIAFAEQRYKNADKAIALFKRIAANNVEFHSG
                     HPQLVELLMAGQGSVCLTCYTHHLLPRMHKTAPLNYLRSLGVGLISATAVFKNAPHPN
                     TAWLFHRWAASDEGQKAFGIEGRVPANPKVEPAEPIGLTTVYGLGEKEYREFARYEKI
                     WKQIFSLR"
     gene            3473..>4408
                     /locus_tag="VE170_14705"
     CDS             3473..>4408
                     /locus_tag="VE170_14705"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16744.1"
                     /translation="MKNPKLLFPNFFVMALVGFFALATDNFAADKLRISYSAVNATQA
                     FLWVAQDRGIFAKHGLEGELLYINSGTMNIAALLGGSVQIAGGGPVSIEARLRGIKLT
                     ILGNPLPWLASNLIVHPDIKSIPDMAGKLAGISRFGSSTDQGFRYLFRKNGLNVDRDL
                     KMLQMGADSSRVAALKTGTIHYTFLGAAATDNARALGFRVLATAQQMAIPFPWTSVVV
                     EDGWLLKNRELAYRYMKCATEAIVTLKRNRADSERIIAKYMKITDPKLIATEFEFVSS
                     LMPDYMAPTLDGLKVILENFGKEYPDAPKRDPKEFA"
BASE COUNT          893 a         1317 c         1169 g         1029 t
ORIGIN      
        1 taagtcgcgt caatgtgtcg cttgcataaa ggacaattgt tgggaaagcg atcgacatac
       61 agtctgatcg gttcagtccg atgattaatc gcggtgatca ttattggcat atttcgattt
      121 ccgcgcgtct aactggcaat aaagggacga ggaattggcg ttatatgtgg ctaactttat
      181 tcctcgcctt cacccgctga ttaagcgact gcaaaactcc cgcgtccacc gccgagatta
      241 catcaactgc agcgtcgagt tcttcttcgg tcatccccgc tttccttgcg tccgcaaggc
      301 gccgatccac tcacggatcg cacaaccgca tcatcgccac ggcgagcccc atgagcatct
      361 tctcgcgctc gctcagacac tcgacggcgt gagcgtcgcg ataaaaagtt cgaaaatctg
      421 ctttttttga ggccatgagt aaattcccta atgatcaatc ttgggatcgc gattctttgg
      481 tccaattgcc ctcacccttc cctctcccat cggaatgggc gagggttaaa actcgaaacc
      541 cggaactagg aactcgaaac tattttacgc cggcttcacg ccggtgatca gcgcgtagga
      601 aaaactgcct ttcacatggg cgtgatcgcc gtgggaatgc caggtgaaat agtttttctt
      661 catgcgctcg gccaactccg gcgcttcctt ttccagctcc agcgccgctt cgagccagtg
      721 gcggccggtg gggatgccgt ccatgccttc ttggttgaag gggtaggaca gcacgctggt
      781 gcgcggcacc cggtagcccg ccttggtcag caaacgctgg atgctgctca ccgtgtggaa
      841 gcgaaagcct tcgccatggg tgcggcgaaa tacctggttg atcttggcct ccagcgccac
      901 gtcgatggcg tccttggatt cttccatgac cgggtcgagc acgacaaagc ggccgcttgg
      961 tttaagcacg cgctggacct cgccgacgac gccggcggga ttgccgaaat gatgcaggct
     1021 gaaggatacg aaggcgcgat cgaaggtccc cgacttgaac ggcaacttgt ccgcttcgcc
     1081 ggcgctcaat tcatagcgcg cgatcggatc tttatgatga tggccgacgt cgagcatcgc
     1141 cagcgcctcg tcgacgccga cgatgcggcc cgactgcatt tgcttcgaca atggcctggc
     1201 aacccgcccc gtgcccgtgg ccagatccaa aatcgcttcg ccgcccttta acgtcagcgc
     1261 ctcgatcaac ttggccgtca gctggccgcg catgtccgac aggccgcgct ggtcgaactc
     1321 ggcggcatga gcaggatcct tgaaacgcga tttgtgatcg tgagaatggg aatcgccgtg
     1381 gccgtgggaa tgaccctgct gtgcgtggtc atgtttctcg ccgtgatcgt gatcgtggcc
     1441 gtgcccatgc tcttgttcat gcccatgatc cgaaccgtgc gcgtgctcat gcccatgcgc
     1501 ttcatgccca tgttcgtgac catgcgcttc atgagaatgg ctgtggccat gtttgtcgtc
     1561 atgatgctcc tcaccatgat cgtgcgaatg ctcaccctta tgctcgtgtt tatgttcgtc
     1621 ggccattgtt ctcttcctat atttagcttt tgccttttta ctttttcttc gacatttcca
     1681 ccatcgcgtc ccattgctcg tccgtcacct tcatgatcga cagccgcgaa ttgcgcacga
     1741 gatcgaaatt ggccagcttc ttgtccgctt tgacttcggc cagggtcacc ggcttatcga
     1801 gggctttcac cgcctcgatg tcgaccacca gcatgcgcgg attgtccttg cccggatcgg
     1861 aatatgcgcc gcccaaggcc cgggcgatgc ccaccgcctg tttctcgtcg ccggtatgat
     1921 agataaaaat ctgatcgccc tttttgatat ccttcaaatg catcagcgcg agattgttcg
     1981 ccaccccgtc ccacatggtc gatttgtctt tgaccagctg ctggtacgaa taaacactcg
     2041 gttcggtctt gaataaccaa cgggttgcca tgaaactctt cctcctacac gagcgttata
     2101 aatcaccagc gcgtactaat acaaactctt gacgatcggg ccctttgtgg cgattttacc
     2161 agcgaaggag ggattgattc aaagctggcg ctcgttcatc catgtgagct atggagatag
     2221 ccgcgcgact gaatcgcgcg gcgtaagaac cgcaggaggt taaccaaccg acctatagaa
     2281 taacggcgat gcggctctat cgtcgccaag ccgtattaag gttacctcaa gctgaaaatc
     2341 tgcttccaaa tcttctcgta tctggcgaac tcccggtact ctttctcgcc caagccataa
     2401 accgttgtga ggccgatggg ctccgccggt tcgactttcg gattcgcagg cacgcggccc
     2461 tcgatgccga acgccttttg cccttcgtcc gaggcggccc agcggtgaaa caaccaggcc
     2521 gtgttcgggt gaggcgcgtt cttgaacacc gccgttgcac tgatcaagcc gacgccgagg
     2581 gagcgcaggt aattgagcgg ggccgttttg tgcattcgcg gcaataaatg atgcgtgtaa
     2641 caggtcaggc agaccgagcc ctgtccggcc atcagcagtt caaccagctg cgggtggccg
     2701 ctgtgaaact ccacgttgtt ggcggcgatt cttttgaaca aagcgatggc tttgtcggca
     2761 ttcttatatc tttgttcggc aaaggcgatg aggaactcgg cgtcgcgcgg ctcggccaag
     2821 agcttgcctt tccatttcgg ttcggcaaac tcttcaaagc tcttcggttc ctccgcttta
     2881 ccgaccaact gcgtgttcca cgccgctacc aaggtattga aacgggtcgc gacccagtaa
     2941 gttcctttga gatgaccggc aaaaacgtcg gcctccggcg gcagcttttc gagcaccaga
     3001 ccctgctcat gaacctgcat gatattttcg aggttcatgt ggaacacatc gccgatagtc
     3061 cggccgccgc gcgcttcgga gatcgcccgc gcaacgattt tatcggagct cgctccggtg
     3121 ttgactgtct tgatgccggg aaagcgtttt tcgaagattg gcaaaacttt ctcggcggta
     3181 tccggcgtca aagtgccgta gacgttcaac acgccgcctt ctttcagtgc ttgtttgtaa
     3241 gtctcgtcga tgctctgggc acgaccggaa ggggcagaga aaaaaaccgc cgcacacaaa
     3301 aacataaagc aacaaatgct tcgcatcgat gcttcgccgc ttagctgcat aggacctccc
     3361 tactggaacg cctgtcctaa cagaccgtct ttcgagttgt caagaaagtt cgttctgtca
     3421 tagtggtaca agcccttgac cgcggtggtc gaatcgctat atccacagtc ccatgaaaaa
     3481 ccccaaactt ctgtttccga actttttcgt catggcgttg gtcggcttct ttgcgctcgc
     3541 caccgataat ttcgccgctg acaagctgcg catctcttat tcggcggtga acgccactca
     3601 agcatttctc tgggttgccc aggaccgcgg catttttgcc aagcatggcc tcgagggcga
     3661 gctgctctac atcaatagcg gcaccatgaa catcgccgcg ttgctcggcg gcagcgtgca
     3721 gatcgccggc ggcgggccgg tgtccatcga agcgcggctg cgcggcatca agctgacgat
     3781 cctgggcaat ccgttgccct ggctggcttc gaatctgatc gtccatcccg acatcaagtc
     3841 catccccgac atggccggca agctcgccgg catctcgcgc ttcggctcct ccaccgatca
     3901 gggcttccgc tatctgtttc gtaaaaatgg tttgaacgtc gaccgcgatt taaagatgtt
     3961 gcaaatgggc gccgactcca gccgcgtcgc ggccctgaaa accggcacga ttcattacac
     4021 attcctcggc gccgccgcca ccgacaatgc gcgggcgctg ggcttccggg tgttggcgac
     4081 cgcccagcag atggcgatcc ctttcccctg gacttcggtg gtggtcgaag acggctggtt
     4141 gctgaagaat cgcgaactcg cctaccgcta tatgaaatgt gccaccgagg cgatcgtcac
     4201 cctcaaacgc aatcgcgccg acagcgaacg catcattgcc aagtacatga agatcaccga
     4261 tcccaaactg atcgccacgg aattcgaatt cgtctcgtcg ctgatgcccg actacatggc
     4321 gccgacgttg gatggactca aagtcatcct ggaaaacttc ggcaaagaat atcccgacgc
     4381 gccaaagcgc gaccccaaag aattcgcc
//
LOCUS       DASQKS010000576         4533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4933598, whole genome shotgun sequence.
ACCESSION   DASQKS010000576 DASQKS010000000
VERSION     DASQKS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4933598"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1251..3266)
                     /locus_tag="VE170_14710"
     CDS             complement(1251..3266)
                     /locus_tag="VE170_14710"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HYJ16745.1"
                     /translation="MSRNQIRLEFESKSSFAGGKTFGAVGPYERQLGMIHFAIDPNEN
                     GLPFVCDIEYAPRNGDGLVEFKAVIDLVKPVDMSKSNGKLLFDFSNRGGRGAFTRLND
                     GGGDYSKAECAGNGFLNRQGYTVVTVGWQGDLVYSGSNVVAFVPEARQNGQPLRGKVR
                     QEFISDKKGVLSLPVSGAANIQCYPVLNRATATLTLREKEADPRMAVPASEWELAKAK
                     EKDGNIELTPSNVDLYVKGGFKPGWIYEMIYDTEGSRVMGLGFLGLREAVSFLRYGGK
                     DSAGVANPLAGAINKVYGYGASLAGRVVREFVYEGWNRDAQGRRVFDAVQTHTGIGRL
                     YCNMRFAQIGRYPRQHEEHSYPSERYPFNFAPIPDPFTGKLDAVLKRPESDPLLVHCH
                     TSSEYWERHGSMTHTDPRDGSDAEIPTNVRMYMLAGSPHAAIAADNPKWVGQLPPSNF
                     SPQPFLRACFMLMDRWASTGVAPPSNRVPRRSDGQLVSPEEALKRFPKIPGVNLPGTP
                     SRLPFWNYGPEFDERGVMTVFPPEAVAGKEYPIQVPQVDTDGNDLGGVRYPDMQAPIA
                     TYIGWALRKAGFAEGELMMTNGCLKPFARTKAEREKIGDPRLSIEERYPSHQAYVDIV
                     KRAVDALVKEGFLLPEDGEDYVAAAKRKNPLDPTVTIEPLVTAGRED"
     gene            complement(3406..>4533)
                     /locus_tag="VE170_14715"
     CDS             complement(3406..>4533)
                     /locus_tag="VE170_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ16746.1"
                     /translation="HVIGIAAQYNPHIMPGATAPLKSFRSEGNIDSTKPTMRLRAHKA
                     VIVATGGSTSDVNFRRMFDPRLTPVFQVGGEPYSYQDASGELAAMAVGASLWGLVHQA
                     LENGATMRSQRALATKYNYTTWRLESPIFPLVKATGFNVKDWQDLILVNQVGKRFYDE
                     TKGDPPHGSHGHQYPDCKPNDYRNAENVKYNPTRYNFVNAALAMNEYSEAPDYCAGPQ
                     WAIFDADAVEREGWKVVPPYVDPDGYFFTGNTLAELAAAIKNEYQAKPMSAAALQATV
                     ARYNSFVDAGVDADFGKPAPKFKIQKPPFYAAWGTVIVHDTRAGLRINARCQVVDMQG
                     QVIPGLYCAGESAGGFNQHGMGRCVTQGYICGGDAAKLPRH"
BASE COUNT          991 a         1294 c         1211 g         1037 t
ORIGIN      
        1 ctgggtgttg caaatccggg acaacgaatc gtgtttgtta gcgattcgcg attaacaagg
       61 cctgcatctt cgagatgcga cgccaaagcc tcgggcgact caagatcgtc atgcgttaaa
      121 tgcgcatctc tacacttcgc acggaaaatt atatttaagc cagagctttg ctcaacgact
      181 agtttgaggt tttttgatgg tctcgaacgc tagtgggact tctagactac cgattgattc
      241 caaagtcatg ccggtgcagt ctttgaaaaa cttcaacatg tactcaccca aggtttcgta
      301 ttccaacaga aggcaaccgg tatattcggc gccctcatgc tctatcgtta aaaatatttg
      361 acgatggttg tatggatagc aacgtgcatt gcgcagaacc ccaacttctc ctctaagact
      421 agattcgctt agtccctggt aatcaatcca gattggtggc caacgggtgt agccgaaata
      481 catcattatg ggatgattgc ggagttgcac agtgacttcg ctcgcaaaat caatgccttg
      541 gacgtatttt gtctaaatca aaataatttc agctacctaa aaaattttct tcggcgactc
      601 cttcgatctc attgtttcga aattgggatt tttttgcagc aatgagacgg taaacgattg
      661 gatcatcccc gacccttgac gttcgaatca tcactgttta attgcgattc ggattaaaac
      721 atcatgagca tgctagtaat aatatccgga cgaggttaat tgcttacagc agcatttagc
      781 gcccgattcc agtctcaacg aaaaactgaa aaggtcatgg catttgaccc caaacgcatt
      841 gcctaacgaa ttatccgcca acgcgattgt ctgcaccgag cttgaggccc atgaattatt
      901 gcgtatcgct ctagagcacg gctctcagaa ggcggttcag gaaattcaaa gcgccatgag
      961 actttgtggc attttatagt tccgttgcgt cgtgagtcga ggtaatcgac ataaagacat
     1021 ttaccttgac agtatcgcaa gtttcatctg tgttgcactt aaccattaac taagtagttg
     1081 ctacctctga aactttatcg tgagcctgac aatgaaccag tgatttccct agctgatttc
     1141 aacggaagct aacgcagtta aacctggcga gcagaatcaa gatccagcgc tgcgttcgat
     1201 atgcgctgca agctaacgaa cagtaaccca agaccatcgg gttgaattat ctaatcctcc
     1261 ctgcctgccg ttaccaacgg ctcgatcgtc accgtagggt caagcgggtt cttccgcttg
     1321 gcggctgcga cgtagtcctc gccatcttcc ggcagcagga aaccttcttt gaccaatgca
     1381 tcaacggcgc gttttacgat gtcaacatac gcctgatggc tgggatagcg ctcctcgatc
     1441 gacaagcgcg gatcgccgat tttttcgcgc tctgctttag tgcgcgcgaa gggcttaaga
     1501 caaccgttgg tcatcatcag ctcgccttcg gcgaagccgg ctttgcgcag cgcccagccg
     1561 atataagtcg cgatcggcgc ttgcatgtcc ggatagcgca cgccgcccaa gtcgttgccg
     1621 tcggtgtcca cctgcggcac ttggatcgga tattctttgc ccgccaccgc ttcgggggga
     1681 aaaacagtca tgacgccgcg ttcgtcgaac tccgggccgt agttccaaaa aggtaagcgg
     1741 cttggcgtcc cgggcaaatt gacgccgggg atcttgggaa agcgtttgag cgcttcctcg
     1801 ggtgacacca attgcccatc agagcgccgc ggcacgcggt tggaaggcgg cgccacgccg
     1861 gtgctcgccc aacggtccat gagcataaag caggcgcgca ggaacggttg cggtgaaaag
     1921 ttgctcggcg gtaactgacc gacccatttg ggattgtcgg cggcgatcgc cgcgtggggc
     1981 gaccccgcga gcatgtacat gcgcacattg gtaggaatct cagcgtcgct gccgtcgcgc
     2041 ggatcggtat gggtcatcga accgtgccgt tcccaatact cgctcgaggt atgacagtga
     2101 accagcaacg gatcgctctc gggacgtttg agcacggcgt ctagtttgcc ggtaaaaggg
     2161 tcgggaatcg gggcaaaatt aaaagggtaa cgctccgacg gataggaatg ttcctcatgc
     2221 tggcgcggat agcggccgat ctgggcgaag cgcatgttgc aatacaaacg gccgatgccg
     2281 gtgtgcgtct gcaccgcatc gaaaactcgg cgtccttggg cgtcgcggtt ccagccttcg
     2341 taaacgaact cccgcaccac ccggccggca agcgacgcac cgtaaccgta aaccttgttg
     2401 atagctccgg cgagcgggtt ggccacgccg gcggaatcct taccgccata acgtagaaac
     2461 gaaactgcct cccgcaggcc gagaaaccca agacccatga cacgcgagcc ctcggtgtcg
     2521 tagatcatct cgtagatcca cccgggcttg aaaccgccct tgacgtagag atccacgttg
     2581 gacggcgtca gctcgatgtt gccgtccttt tccttcgcct tggccaattc ccattcgctc
     2641 gcaggcaccg ccatgcgcgg atcggcttcc ttttcacgca acgtcaaagt tgccgtggcg
     2701 cggttgagca ccgggtagca ttgaatattc gccgcgccgc tcacaggcag ggacaacacg
     2761 cctttcttat cggaaataaa ctcctggcgc actttgccgc gcaaaggctg tccgttctga
     2821 cgcgcttccg gcacgaatgc gacgacattg ctgccgctat aaaccaaatc gccttgccat
     2881 cccacggtga caaccgtgta gccctggcga ttgagaaaac cgttgccggc gcattctgcc
     2941 ttggaataat cgccgccgcc gtcgttcaag cgtgtgaacg cgccgcggcc accgcgattg
     3001 gaaaaatcga acagcaattt cccattgctt ttactcatat cgaccggttt gactagatcg
     3061 atcacggctt tgaactcgac caaaccgtcg ccattgcgcg gcgcgtattc gatatcgcaa
     3121 acgaacggca agccgttttc attgggatcg atagcgaaat ggatcatgcc cagttgccgc
     3181 tcataaggcc cgacggcgcc gaacgtcttg ccgccggcga aactgctctt gctttcgaac
     3241 tccaggcgaa tttgattgcg tgacatcttc atcttccttt cgactttcga ccctatgacc
     3301 caacgtgttc acaccgcgac aaaaaccttg aatcttgatg ggtaataggc gacgcgtgag
     3361 gtgccaggga acgttaatcc ctggcgtctc acccctaacg attttctaat gccgcggcag
     3421 cttggccgca tcgccgccgc aaatataacc ctgggtgacg cagcggccca tgccatgctg
     3481 attgaacccc ccggccgatt caccggcgca gtatagtccg ggaatcactt gtccttgcat
     3541 gtcgaccacc tgacagcgcg cgttgatgcg caaacccgcg cgcgtgtcat ggacgatcac
     3601 cgtgccccac gccgcgtaga acggcggctt ctggattttg aacttgggcg cgggcttgcc
     3661 gaagtcggca tcgacaccgg catcgacaaa cgagttgtaa cgcgccaccg tcgcttgcaa
     3721 cgctgcggcg ctcatcggct tggcttgata ttcattcttg atcgccgctg ccagttcggc
     3781 gagtgtgttg ccggtgaaaa agtaaccatc ggggtcgacg tagggcggca ccaccttcca
     3841 accttcgcgc tccaccgcgt cggcatcgaa gatcgcccac tgcggcccgg cgcagtaatc
     3901 cggcgcttcg gagtattcat tcatcgccag cgctgcgttg acgaaattgt atcgagtcgg
     3961 attgtatttg acgttttccg cgttgcgata atcgttgggc ttgcaatcgg gatattgatg
     4021 tccgtggctg ccatggggcg gatcgccctt ggtctcgtca taaaagcgct tgccaacctg
     4081 gttgacgaga atcaaatcct gccagtcctt gacgttgaaa cctgtggcct tcaccaacgg
     4141 gaagatcgga ctctccagcc tccaggtcgt gtagttatac tttgtcgcca gggcgcgctg
     4201 ggagcgcatc gtcgcgccgt tctccagcgc ctgatggacc aaaccccata acgacgcacc
     4261 gaccgccatg gcggcgagtt caccgctggc gtcttgataa ctgtagggtt cgccgcccac
     4321 ttgaaacacc ggcgtcagac gcggatcgaa catccggcgg aaatttacgt cgctggtgct
     4381 accgccggtg gcgacgatga cggccttgtg cgcccgcaac ctcatcgttg gtttggtgct
     4441 gtcgatattg ccctcggagc ggaaactctt gagcggcgcg gtcgcacccg gcataatatg
     4501 cgggttgtac tgcgcggcga tgccgatcac atg
//
LOCUS       DASQKS010000577         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1174834, whole genome shotgun sequence.
ACCESSION   DASQKS010000577 DASQKS010000000
VERSION     DASQKS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174834"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VE170_14720"
     CDS             <1..325
                     /locus_tag="VE170_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16747.1"
                     /translation="SSAANVKTFINMRPSVVIPDVLASYKLMKSLNPEIVIAGHGQPT
                     TTKIFDEYEGYYTLLMKRVGEMAAKGMTLDQVKQELKMPEYADWQGQDRLPVNIEQAW
                     KVVKK"
     gene            343..1155
                     /locus_tag="VE170_14725"
     CDS             343..1155
                     /locus_tag="VE170_14725"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16748.1"
                     /translation="MGYLNRIVVACWVASLCDVVGAQEIVTLPTRAGVTQSYFLTSSP
                     KQLQAIAVLFPGSGGLIQLRSEGGQPKFAGGNFLVRSRSEFIKRGVIAAILDAPSDLQ
                     SGWGMSDEFRLGGDHFTDISAVVADLNKRFPGVAVFLIGTSRGTISAAALGARFGPRI
                     AGAVLTATMFREVGRNSNEPGPGLSRFDFATIQIPLLFAHHVSDQCRYTPYGDAARYS
                     EKYPLITVWGGLPPQSTPCEARSQHGFLGKESETVEQIVNWMLKKPFLYEVK"
     gene            1164..2111
                     /locus_tag="VE170_14730"
     CDS             1164..2111
                     /locus_tag="VE170_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ16749.1"
                     /translation="MVTLPLNEVDRVEILSVMDNSIDVLMGNTPVARRHQRGSNAHAR
                     PQLRAEHGVSMLVTTYSAGQKDSFLFDTGVTVDGVLHNMDLLDIKGNELHAVVLSHGH
                     TDHTRGLLGFIKRYGRPQVPIVLHPDAYLKRKNIQGDGHESEHIPPSKKDLEGEDIQI
                     VEERGPTMLIGGNALVTGQIPRTTTFEKGSPNQVALIDGKWQPDPWIHDDQAIVINVK
                     NKGLVVLTGCGHAGVINTLKCARDMTGVKQIHAVIGGFHLTGPIFEPIVQPTVQALKE
                     FNPGVIVPQHCTGWKATHLIAREFPEAFTPNSVGTTMVI"
     gene            2384..2761
                     /locus_tag="VE170_14735"
     CDS             2384..2761
                     /locus_tag="VE170_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16750.1"
                     /translation="MAKLRHLALLTHQPDKLADYYKRVFDMQELYRTKNGSVHLSDGE
                     VNLAILNANDPKEPGHPAGLYHFGFHIDDAQETTKRIMDVYPEGAPKDRIATYAEGRA
                     ADPDGNLIDISTTGWGPVRRNDL"
     gene            2775..3854
                     /locus_tag="VE170_14740"
     CDS             2775..3854
                     /locus_tag="VE170_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16751.1"
                     /translation="MRLIIYFLLVCGLIVGPGSGASAASIDEVVKKASALSGAERKTF
                     LEAGAKKEGEVVFYTSLSLTDYPKILPHFEKAYPFLKTNTYRATPSGLFTRVDTEARA
                     GRFAADVIGSAPVEMWQLKQRKLSAPYLSPERRALQPGAFDPEGFWQAFDVTPLVLGF
                     NTKQVPAADAPRSYADLLHPKWKGKMSLGTEEYTWFNVLLETMGKAKGSELMQGLAKQ
                     DLAMPGSSSVMRVQLMLAGESAIAIAARGRRVSEYKQQGAPIDYRVLDPYAGEPNFVT
                     LMQRAPHPFAAVLFIDWMLSEEGQTRMAEVGRLSVRRGIKHRAWVQELFQKDFVFLSP
                     SSIGPNLNEIIDQYNRIFGVQRRTK"
BASE COUNT          849 a         1167 c         1173 g          802 t
ORIGIN      
        1 atcctcggcg gccaacgtca aaacttttat taatatgcgg ccgtcggtgg tgatccccga
       61 cgtactggcg agttataaat tgatgaagtc gttgaacccg gaaatcgtca ttgccggcca
      121 tggccagccg accacgacca aaatcttcga cgagtacgaa ggctattaca ccttgctgat
      181 gaaacgggtg ggcgagatgg cggccaaggg aatgactctc gatcaggtta agcaagagct
      241 caagatgcct gagtatgccg actggcaggg gcaagatcgc ctgccggtga atatcgaaca
      301 ggcgtggaag gtcgtcaaga agtaagtttc catcgcgtgt tcatgggtta ccttaatcgc
      361 attgtcgttg cctgctgggt tgccagtctt tgtgatgtcg ttggcgccca ggaaattgtc
      421 accttgccga cacgcgcagg cgtgacccag tcctatttcc tcaccagctc gccgaaacag
      481 ctgcaagcga ttgctgtctt gtttcccggc tccggcggct tgattcagct tcgcagcgaa
      541 ggcggccagc cgaagtttgc cggcggcaat tttctggtcc gcagccgttc cgagttcatc
      601 aagcgcggcg tcatcgcggc gatcctcgac gcgccctcgg acctgcaaag cggctggggc
      661 atgagcgatg agtttcgcct cggcggcgat cacttcacgg atatctccgc cgtagtcgcc
      721 gatctcaata agcgcttccc cggcgtggct gtgtttctca tcggcaccag ccgcggcacc
      781 atatcagcgg cggcgctcgg cgcccgtttc ggtccaagaa tcgccggcgc cgtgctcacg
      841 gcgacgatgt ttcgcgaggt cgggcgaaac agcaacgagc ccggccccgg cctcagccga
      901 ttcgacttcg cgacgattca aatcccgctc ttgtttgcac accatgtcag cgatcaatgc
      961 cgttacacgc cctacggcga cgccgcgcgc tattccgaaa agtatccgtt gatcaccgtg
     1021 tggggcggtt tgccgccgca gtcgacgccc tgcgaagcgc gcagccagca tggttttctc
     1081 ggcaaggaat ccgagacggt cgagcagatc gtcaactgga tgctgaaaaa gccttttctc
     1141 tacgaagtaa aataggaggc cctatggtga ccttgccgct gaatgaagtc gaccgcgtcg
     1201 aaatcttgag cgtgatggat aattccatcg acgtgctgat gggcaacacg ccggtggcca
     1261 ggcgccacca gcgcggcagc aacgcccatg cgcggccgca gttgcgcgcc gagcatggtg
     1321 tgtcgatgct ggtgacgact tactccgccg gccaaaaaga ttcgtttctc ttcgacaccg
     1381 gcgtgaccgt cgacggcgtg ctgcacaaca tggacttgct cgacatcaag ggcaacgagc
     1441 ttcacgccgt cgtcttgagc catggccaca ccgaccacac gcgcggtctg cttggattca
     1501 tcaagcgcta tggccggccc caagtgccga tcgttctcca tcccgacgcc tatctgaaaa
     1561 gaaaaaatat tcagggcgac ggccacgagt cggagcatat tccgccgagc aaaaaggatc
     1621 tcgaaggcga ggatattcag atcgtcgagg aacgcgggcc gaccatgttg atcggcggca
     1681 acgcgcttgt caccgggcag atcccgcgga cgacaacctt cgagaaaggc agccccaacc
     1741 aagtcgcgct gatcgatggc aagtggcagc ccgatccgtg gattcacgac gatcaagcga
     1801 tcgttatcaa tgtgaagaac aaaggactcg tcgtgcttac cggctgcggc cacgccggcg
     1861 tgatcaacac gttgaaatgc gcgcgcgaca tgaccggagt aaagcaaatc cacgcggtca
     1921 tcggcggctt tcacttgacc gggccgatct tcgaaccgat cgttcaaccg acggtgcagg
     1981 cgctgaaaga attcaatccc ggcgtcatcg tgccgcagca ttgcaccggc tggaaggcga
     2041 cgcatctgat cgcgcgcgag tttcccgagg cgttcacgcc gaacagtgtt ggcacgacga
     2101 tggtcatcta gcgcgcatcg cgcgctacaa gcgttcaaat cgttcaagcc gttcaacccc
     2161 tgtgctccgt tcaagccgtg tcggaaacag gaggttacaa caaatttagg cgaaaccagg
     2221 acactcccgc gtcagagtcg cgactatccc gtttgcgttt ccgaagacga ttggaaaggt
     2281 ttgaacggct ggaacgattg gaacgccctt gccgactacg ttgacaggct atttctgcgg
     2341 tgatagcgct ggcatgtctt gcaaataaat aggagggacg atcatggcaa agcttcgaca
     2401 cttggcgctg ctgacgcatc aaccggacaa attggcggat tactacaagc gtgtcttcga
     2461 catgcaggag ttgtatcgga ccaaaaacgg ctcggtgcac ctgtccgacg gcgaggtcaa
     2521 tctcgcgatc ttgaacgcca acgatcccaa agagcccggc catccggcgg gactgtatca
     2581 cttcggcttc catatcgacg atgctcagga aaccaccaag cggatcatgg acgtctatcc
     2641 cgagggcgcg cccaaggatc gcattgcgac ttacgccgaa gggcgggccg ccgatcccga
     2701 tggcaacttg atcgacatct cgacgacggg ctggggcccg gttcgccgca acgatctgta
     2761 agcgcggctg gcgcatgcgg ctgattattt acttcttact tgtctgcggc ttgattgttg
     2821 ggccggggag cggcgcgagc gccgcctcga ttgacgaagt cgtcaagaaa gcctcggcgc
     2881 tgtccggcgc cgagcgcaag acttttctcg aagctggggc gaagaaagaa ggcgaggtcg
     2941 tcttctatac ttcgttgagc ttgaccgatt acccaaagat cctgccgcat tttgaaaaag
     3001 cctacccctt tctcaagacc aatacttatc gcgcgacgcc gtccggcttg ttcacgcggg
     3061 tggacaccga agcgcgcgcc ggcagattcg ccgccgacgt gatcggctcg gcgccggtgg
     3121 agatgtggca gctcaagcag cgcaagctat cggcgccgta tctctcgcct gaacggcgcg
     3181 cgctacaacc gggtgcgttc gatcccgagg gcttttggca ggctttcgac gtgacgccgc
     3241 tggtgctggg tttcaatacc aagcaggttc ccgccgcgga cgcgccgcgc agctatgccg
     3301 acttgttgca tcccaagtgg aagggcaaga tgagtttggg caccgaggag tacacttggt
     3361 tcaacgttct tcttgaaacc atgggcaagg ccaagggcag cgaattaatg caaggactgg
     3421 ctaagcagga tctcgccatg cccggttcaa gcagcgtcat gcgcgtgcag ctcatgctcg
     3481 ccggcgagtc ggccatcgcc atcgcggcgc gcggccggcg cgtgtcggaa tacaagcagc
     3541 agggcgcgcc catcgattac cgcgtgctcg atccctatgc cggcgaaccc aacttcgtca
     3601 ccttgatgca gcgggcgccc catccgtttg cagcggtcct gtttatcgat tggatgctct
     3661 cggaagaagg ccagacgcgc atggccgagg tcgggcgctt atccgtgcgc cgcggcatca
     3721 agcaccgagc ctgggtgcag gagctatttc aaaaagattt cgtgttcttg agcccatcgt
     3781 cgatcggacc gaacttgaat gaaatcatcg atcagtacaa ccgcattttc ggcgtgcagc
     3841 ggcgcacgaa gtaaggccta aatttcggat cggttggccg caaagaacgc aaaaggcaca
     3901 tagtcgaaat ctcaggggcg ggtttcaaac ccgcccttat ttgttgtgca cgatatgaag
     3961 attggattat tcactgagtt ttcctatccg g
//
LOCUS       DASQKS010000578         7905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_288842, whole genome shotgun sequence.
ACCESSION   DASQKS010000578 DASQKS010000000
VERSION     DASQKS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288842"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1616
                     /locus_tag="VE170_14745"
     CDS             78..1616
                     /locus_tag="VE170_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16752.1"
                     /translation="MVKKSANLSRRDFLKHSTALGVSALVIPQLSRDARAASRERVTV
                     YHNTVADSIHLYKQSSGSIYGNWQHVFEPLVELDYKKKDWVGVLAESWQFQGTRWVFK
                     LKKGVRFHNGAPLTSRDVAYSVERMRDERGGSLQGSNFKDVTEIQTPDAQTVIFVTKQ
                     PLAIFLDRLRNRFIVSKIAGDKFGDQWFENPIGTGPYKFVSFQRGGNLVFTRNDDYWG
                     GKAQIKDIVFRKVTEDGARVAALESGQADFINDVPVHEVSRLERHPRVRVDRIEGLEM
                     YFLMMNVAYKPFDNKLVRQAVNYSIDAAAIVKNIFDGLGYPTPGPIGANVVGADTKLK
                     RYPYDPQKAKELLAQAGYANGCDVQLYYSAGRYPKDREVCQVAAAQMVKGGFRVELIS
                     QEWALFWGPDGVNGGKLPFYYNNRGSLTDADTFYDQYFRTGTTKRCNYSNPAFDKLID
                     EEQQTADNKKRITLLQQAGKILMNDAPYVPLYNLATIYGAAKNLQWKMRPDEKVMGWD
                     MKIV"
     gene            1806..2840
                     /locus_tag="VE170_14750"
     CDS             1806..2840
                     /locus_tag="VE170_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16753.1"
                     /translation="MSNLEPKGAVHWIDHYVVGTNDLPGWSYWALNATGLPIRPINGL
                     TTNMRKKNTPIFCFMWWDDESCRIGAFLQPENYPKAKELGKDMPRCGFYVRPEDIDSH
                     LRRLDRHKIPHTDPIRNADNGDEGTTICFADPDGNQYEFWAPVSMPQGAMEVRTTENV
                     GRISHCVYGSRDLARTAAFFEKYCGMESERSAKTAEGTLVLRMRGGARLVYKLADQLD
                     ERIAGHSPWWDMHTAFTVRDDEFFPNYRRMWETLPEEAGAKEHLNDSRDKEDNFPART
                     GLHRSPVGYKWKEIYQRGDEFYDWDGHAFHFIGGIPKHADGSLTMYTPKEQDEYLREL
                     AESLKKGVQP"
     gene            3137..3970
                     /locus_tag="VE170_14755"
     CDS             3137..3970
                     /locus_tag="VE170_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ16754.1"
                     /translation="MAKVFSIASWNVEHFKDDLTRVGKVIDLVKAQNPDVFGLYEVEG
                     ARVFNALVDKMPNYTFQITEGLQTQEILVGVSKQLTAFITQKTEFRSGTTHMRPGQLV
                     TIVTNGKNYALLFLHLSSGTDPRGMGLRDDMLERAFEFRKILDSAEGGPGKARYLFLG
                     DFNTMGMKYPFDRSILADVELKKWDNYAARPTIAMRRLKKSHDATFFNGSTSSFPPSN
                     LDNVYASKNLRFKTFSHATTGTPVEVDVRGWVNEPTDAKKDEWIGMFSDHSLLYFEIH
                     D"
     gene            complement(4073..4618)
                     /locus_tag="VE170_14760"
     CDS             complement(4073..4618)
                     /locus_tag="VE170_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYJ16755.1"
                     /translation="MEAAGLVLDALGRVRAMVRVALKDLTPEELLSPPKPHIAWLAWH
                     IARVQDANFAGLLDRPQLWIADGWHRRFNMPPDPRDYGSGHRQTCEQVDAFIVTDKAL
                     LLAYHEAVFAQTRDYLSTVTNADLNRVLDEPQYSPLPTLSIRLASVINCNTRHAGQIE
                     YLRGLVKFKGWFPAAEPGPVS"
     gene            complement(4721..6463)
                     /locus_tag="VE170_14765"
     CDS             complement(4721..6463)
                     /locus_tag="VE170_14765"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ16756.1"
                     /translation="MSNCRVISTIRLPTMLASLALFFGACSLETEMWHVEKQQNASTG
                     QTYESKAPLPSPTPGRPSASTDSLRRTPESTRMGGAIMSPNPYPQPPYYPRPPVVVPN
                     TDRFPDKEPTSVLSAAEHPVSTFSVDVDTASYAFVRRSLTGGRVPPRESIRVEELVNY
                     FPYAYPVPGNRAQPFQVMTTVMPSPWAAERQLLHIAIRGYDIERSERPRVNVVLLIDT
                     SGSMQPSDRLPLLQQSFRMFVQPLRDDDRVAIVTYAGQAQTSLDPTRGRDKQKILDAI
                     DSLRASGSTAGGEGLQRAYALAERHFDKEAVNRVILGTDGDFNVGITNPKDLERLVAA
                     KRKTGIYLSILGVGVGNLNDALMQRLAQAGNGNAAYIDSLLEARKAMSEELSSTMFPI
                     ADDVKIQVEFNPMRVAEYRLIGYETRMLKREDFNDDKVDAGDIGAGHTVTAIYDVTPV
                     GAKQRQLDPLRYQRESERTAPPANLGSDELAFLKLRYKLPGETASRLIEQPVRASDAL
                     AKIASAPAEQRFAVAVAAFGQRLRGENAVDSFSYGKIAELANGARGADSEGYRAEFVR
                     LVRMAETLGAVAQR"
     gene            complement(6683..7756)
                     /locus_tag="VE170_14770"
     CDS             complement(6683..7756)
                     /locus_tag="VE170_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16757.1"
                     /translation="MLAATAGLAAAQTNWQAEWDKTVRAAESEGQLTLYGCCYDYDRV
                     LEVFKKKYPKLKVSTVLSGGQLAARILAERRGEKYLVDVVSSGANTLHDALHKAHALD
                     PIKPALLLPEVVDQSKWYDGEHRYIDTERRYIFAFNANSQSGQITYNLKQVNPGEFKS
                     YWDLLNSKWKGKLLSLDPTSFGMGTTLQFYYHHPELGPPFLKKLYGEMQITTGRDPRQ
                     MTDWLATGKFPVCIRCNAGSEIGKAVQQQLPIGYFDTDEWKEGGSLSAAGGTLGIPTR
                     APHPNAARVFINWLLSREGQIAVQKLGRPDAHNSRRIDIPKDDVDPTNRLDPAKKYLD
                     LAKPEFQDLTPIFKLVKEVLPQK"
BASE COUNT         1748 a         2325 c         2221 g         1611 t
ORIGIN      
        1 tacggaatca taacttcccc gaacctgcgt gcccttctcg tgacaatttc ttctcgtaat
       61 actccgtcgg agggactatg gttaaaaagt cagccaactt aagccgcaga gattttctca
      121 aacattctac cgcgctcggc gtcagtgccc tggttatccc gcagctctca cgggacgcgc
      181 gcgccgcgtc gcgcgagcgc gtgaccgttt atcacaacac cgtcgccgac tcgattcacc
      241 tctacaaaca aagctcgggt tcgatctacg gcaactggca gcatgtcttc gagccgctgg
      301 tggagctcga ctacaagaaa aaagattggg tcggggtgct cgccgagtct tggcaattcc
      361 agggaacgcg ctgggttttc aaacttaaaa aaggcgtgag gttccacaac ggcgcgccgc
      421 tgacttcgag ggacgtggcc tattcagtcg aaagaatgcg cgacgaaagg ggcggcagcc
      481 tgcaaggctc gaacttcaaa gatgtaacgg aaatccagac cccggacgct cagacggtca
      541 tctttgtcac caagcagccg ctggcgatct ttttggaccg attgcgcaac cgcttcattg
      601 tcagcaagat tgccggcgac aagttcggcg accaatggtt cgaaaatccc atcggcaccg
      661 gcccctataa attcgtcagc ttccagcgcg gcggcaattt ggtatttact cgcaacgacg
      721 attattgggg cggcaaggca cagatcaaag acattgtctt ccgcaaagtt actgaagacg
      781 gcgcgcgcgt ggcggcattg gaatcgggtc aagcggactt tatcaatgac gtgccggtgc
      841 acgaagtatc gcgtttggag cgccatccgc gtgtccgcgt cgaccgcatc gaaggcttgg
      901 agatgtattt cttgatgatg aacgtcgcct acaagccttt cgacaacaaa ctggtgcgcc
      961 aagcggtgaa ctatagtata gacgccgcgg ccatcgtcaa aaatattttc gacggcctgg
     1021 gttacccgac gccgggcccc ataggcgcca atgtcgtcgg cgccgatacg aagctgaaac
     1081 gttatccata cgatccgcag aaagccaagg agctcctggc gcaggccggc tatgccaatg
     1141 gctgcgacgt ccaactttat tactccgccg gccgttaccc taaagacaga gaagtctgcc
     1201 aggtggccgc ggcgcaaatg gtcaaaggcg gcttccgcgt cgaactgatc tcgcaagagt
     1261 gggcgttgtt ctggggaccg gacggcgtca acggcggcaa gctgcccttt tattacaaca
     1321 accgcggctc gctcaccgac gccgacacct tctacgatca atacttccgc accggcacga
     1381 ccaagcgttg caactacagc aacccggctt tcgataagct catcgacgaa gagcagcaaa
     1441 ctgccgacaa caaaaagcgc atcacgctac ttcagcaagc gggtaagatt ctcatgaacg
     1501 acgcgcccta cgtgccgctc tacaacctcg caaccatcta cggcgcggcg aaaaacctgc
     1561 aatggaaaat gcgtccggac gaaaaagtca tgggttggga catgaagatc gtctgattcg
     1621 agtccaagct caatcgagtt gaaagttcga aattaacccg gcaaggaatc tcgctgtcca
     1681 catgcaacca taaacgcctg aacctttgaa ccaagacttg tcgtagactt tacttgacac
     1741 cctgttaact gtctgttacc tcaagcacaa tctaacctat cactttccct actggaggac
     1801 tttccatgtc taacctcgaa cccaagggcg ccgttcactg gatcgatcat tatgtcgtcg
     1861 gcactaacga tttaccgggc tggagttact gggcactcaa cgctaccggc ttgccgattc
     1921 ggccgattaa tggacttacc accaacatgc gcaagaaaaa cacgccgatc ttttgcttca
     1981 tgtggtggga cgacgagtcg tgccgtatcg gcgctttttt gcagccggag aattacccca
     2041 aggcgaagga gcttggcaag gatatgccgc gctgtggttt ttacgttcgg cccgaagaca
     2101 tcgactcgca tcttcgccgg ctcgatagac ataagattcc ccacaccgat ccaatcagga
     2161 acgccgacaa cggcgacgaa ggcacgacga tctgcttcgc cgatcccgac ggcaatcagt
     2221 acgaattttg ggcgccggtc agcatgcccc agggcgccat ggaagtgcgc accacggaaa
     2281 acgtcggccg catcagccac tgcgtctacg gttcccgcga tctggcgcgc accgcggcgt
     2341 tcttcgaaaa atattgcggc atggaatcgg agcgcagcgc caagaccgcg gagggcacgt
     2401 tggtcctgcg catgcgcggc ggcgcccggc tggtctacaa gttggccgat cagctcgatg
     2461 agcgcatcgc cggccatagc ccttggtggg acatgcacac cgcattcacc gtgcgcgacg
     2521 atgaattttt ccccaactat cggcgcatgt gggagaccct gcccgaagaa gccggcgcca
     2581 aggaacactt gaacgactcc cgcgacaaag aagacaattt ccccgcccgc accgggctgc
     2641 acagaagccc agtgggctac aaatggaagg aaatctacca gcgcggcgac gagttctatg
     2701 actgggacgg ccacgccttt cacttcatcg gcggcatccc gaagcatgcc gacggctcat
     2761 taactatgta cacgccgaag gaacaggatg aatatctgcg cgagctcgcc gagtcgttga
     2821 aaaaaggcgt gcagccgtag tcccggcaaa cggctccgcc gtcgactccc aagcaccact
     2881 cggcggaagt agcccgaagc acgcgaaggg ttacatcgat agcataaatg cgttctcacg
     2941 cttagtactt cgccggataa ataacggcta tgccgtggtt gtgaccatcg gatgagcggc
     3001 ggcgcaacgt cgcgctccga gctcatacgg gtgcgacctg cagccatcct gcgatccggc
     3061 tcggcggggg atagcacatc atcaccgcag tcagacatgg cacaacaagc gcttactccc
     3121 caaaggaaca attcccatgg caaaagtctt ttccattgcg tcgtggaacg tcgagcattt
     3181 caaggacgat ttgactcgcg ttggcaaagt catcgatctc gttaaggcgc agaatccgga
     3241 tgtcttcggc ttgtatgagg tcgagggcgc gagggtgttc aacgcgctgg tggacaagat
     3301 gccgaactac acgttccaga taaccgaagg attgcaaacc caggagattc tcgtcggcgt
     3361 cagtaagcag ctgaccgcct tcatcaccca gaagaccgaa tttcgctcgg gtaccactca
     3421 catgcgcccg ggccagctcg tcaccatagt gaccaatgga aaaaactacg cgctgctttt
     3481 cctgcacctg tcgagcggga ccgacccacg cggcatgggg ctgcgcgacg acatgctcga
     3541 gcgcgccttt gaatttcgca aaatcctcga cagcgccgaa ggcggccccg gtaaagcgcg
     3601 ttatctattc ctcggcgact tcaacacgat ggggatgaaa tacccgttcg accggtcgat
     3661 tctggcggac gtcgaattga agaagtggga caactacgcc gcccggccga cgatcgcgat
     3721 gcgacggttg aagaagtcgc atgatgcgac atttttcaac ggcagcacat cgtccttccc
     3781 gccatccaat ctcgacaatg tctatgcgtc aaaaaatctc cgcttcaaaa ccttttccca
     3841 tgccaccacc ggtacgccag tcgaggtcga tgttcgcggc tgggtcaacg agccgaccga
     3901 cgcgaaaaaa gacgaatgga tcggcatgtt ctcggatcac tcgctcctgt atttcgaaat
     3961 tcacgactga aggcggcttg gattctatca ctgagatgac cggccgcgca cacagccatt
     4021 ggccgtgcgc gtcgccttcg gcgcataggc gttaacctca ccggctgccg cgctaggaga
     4081 cgggtcccgg ctccgcggcg ggaaaccaac ctttgaattt caccagtccg cgcagatatt
     4141 cgatctggcc ggcatggcgc gtgttgcagt tgatcacgct ggcgagtcga atactgagcg
     4201 tcggcagcgg cgagtattgt ggttcgtcga ggacccggtt tagatcggca ttggtcaccg
     4261 tcgacagata atctcttgtc tgcgcgaaca cggcctcgtg gtaagcgagc aacagcgcct
     4321 tgtcggtgac gatgaaggca tccacctgtt cgcaggtttg gcgatgacct gagccatagt
     4381 ctcttggatc gggcggcata ttgaagcgac ggtgccagcc gtcggcgatc cagagctgcg
     4441 gccggtcgag taggccggca aagttcgcgt cctgcactcg cgcgatatgc caggccagcc
     4501 aggcaatgtg cggcttgggc ggtgacaata actcttccgg cgtgagatct ttcagcgcaa
     4561 cgcgcaccat tgcgcgcacg cgccccaacg catccaatac cagtccggcg gcttccataa
     4621 gtcctcctcg acattacttt ggtagtcgtg ggcgacttta gtgccgcgtg attgtcgaca
     4681 caagactcaa ttgcgtaaac tttaatcgaa ccctcatcct tcagcgttgt gccaccgcac
     4741 ccagagtttc cgccatgcgc accagacgga cgaactcggc gcggtagcct tccgaatcag
     4801 cgccgcgagc gccgttagcc agctccgcga ttttcccgta actaaagcta tccaccgcat
     4861 tctcaccgcg cagtcgttga ccgaaggcgg caaccgcgac ggcaaagcgc tgctccgccg
     4921 gcgcgctggc gatcttcgcc agcgcgtcgg acgcgcgcac cggctgctcg atgagtcgac
     4981 tggcagtttc gccgggaagt ttgtagcgca gcttcaggaa tgccaactca tccgagccca
     5041 agttggcggg cggcgctgtg cgttcgctct cccgctgata acgcagcgga tcgagttggc
     5101 gctgtttcgc gcccaccggg gtgacatcgt atatcgccgt cacggtgtgg ccggcgccga
     5161 tgtcgccggc gtcaaccttg tcatcgttga aatcctcgcg cttcaacatg cgcgtctcgt
     5221 agccgattaa gcggtactcg gctacgcgca ttgggttaaa ctctacttgg attttcacat
     5281 cgtcggcgat cggaaacatg gtcgaggaca attcttcact catcgccttg cgtgcttcga
     5341 gcaaggaatc gatataggcc gcgttgccgt tgccggcctg cgccaggcgc tgcataagcg
     5401 cgtcgttcag attgccaacg ccgacaccga ggatcgataa atagatcccg gtcttgcgct
     5461 tggcggcgac caagcgttcg aggtcctttg gattggtgat gccaacattg aaatcaccgt
     5521 cggtcccgag gattacgcga ttcaccgcct ctttatcgaa atggcgctcg gctagagcgt
     5581 acgcgcgctg caagccctcg ccgcccgcag tcgacccgga cgctcgaagg ctgtcgatgg
     5641 cgtcgagaat cttttgctta tcccgccctc gggtcgggtc cagggaagtt tgcgcctggc
     5701 ccgcgtaggt gacgatggcg acccggtcat cgtcacgcaa tggctggacg aacatgcgaa
     5761 agctctgctg caacaaaggc aggcgatcgc tcggctgcat cgagcccgaa gtatcgatca
     5821 agagcaccac gttgacgcgc ggccgctcgc tacgctcgat gtcgtaaccg cgaatagcga
     5881 tatgcagcag ctgcctctcc gccgcccacg ggctcggcat caccgtcgtc atcacctgaa
     5941 acggttgcgc gcgattccca ggaacgggat aggcgtaagg gaaatagttg accagctcct
     6001 cgactcgaat agattcccgc ggcggcacgc gcccgccggt gagactgcgg cgaacaaagg
     6061 cgtaggacgc cgtgtcgaca tcgacgctga aggtcgacac cggatgctcc gcggcactga
     6121 gcacgctcgt cggttcttta tcggggaaac ggtcggtatt cggaacaacg actggcgggc
     6181 gcgggtaata cggcggctgg gggtaaggat tcggcgacat gatcgcgcca cccatgcggg
     6241 tggattctgg cgtgcgtcgt agtgagtcgg tactcgcgct tggccggccc ggcgtgggtg
     6301 acggcaacgg cgccttggac tcgtaggttt gcccagtcga agcattctgc tgcttttcca
     6361 cgtgccacat ctcggtctct aggctacagg cgccgaagaa taaggcgaga ctcgctagca
     6421 tagtcggcaa gcgaatcgtg ctaataacac gacaattcga cataaaaatc ccctccgtta
     6481 ggtcgatcgg cacaagtctt acgatattcc ttagacgatt gagaacgggt ttcgttccac
     6541 agcgcacgtt ggatccgctg caagtaaaat cgcgcgatca ataataaaca gcgtcgctgc
     6601 aagccacgat gaatcgacgc acagagattg aagacggagg gttcggggag attcttcccc
     6661 aaatatcgga gcaaaggtga agctattttt gcggtagtac ttctttgacc agtttgaaga
     6721 tcggcgtcag atcctggaac tcgggtttgg ccagatcgag atattttttt gccgggtcca
     6781 agcgattggt tggatcgacg tcgtctttcg gaatatcgat gcgccgcgag ttatgcgcgt
     6841 cggggcgacc caatttctgc accgcgatct gtccttcgcg ggacagcagc cagttgataa
     6901 aaaccctggc agcattggga tgcggggcgc gcgtcgggat gccgagcgtg cctccggccg
     6961 cactgaggct accgccctct ttccattcat ctgtgtcgaa ataaccgatc ggcaattgct
     7021 gctgcaccgc cttgccgatt tcgctgccgg cattgcaccg gatgcacacc ggaaactttc
     7081 ccgttgctag ccaatcggtc atctggcgcg ggtcgcggcc ggtcgtgatt tgcatttcgc
     7141 cgtaaagttt cttgagaaac ggcgggccga gctccgggtg atggtaataa aattgcagcg
     7201 tagtacccat accaaagctc gtcggatcga gtgagagcag cttgcccttc catttggaat
     7261 tcaacagatc ccagtaggat ttgaattcac caggattgac ctgcttgagg ttgtaagtaa
     7321 tttggccgga ctgcgagttg gcgttgaagg cgaatatgta acgcctttcc gtatcgatgt
     7381 aacggtgctc gccgtcgtac cattttgatt gatcgacaac ctcaggaagc agcagcgccg
     7441 gcttgatcgg gtcgagcgcg tgggccttgt ggagcgcgtc gtgcagggta ttggcgccag
     7501 agctgacaac gtccacgaga tatttctcgc cacgccgttc ggcaagaatc cgcgccgcta
     7561 actggccgcc gctaagcacc gtcgagactt tgagtttggg atatttcttt ttgaagactt
     7621 cgagaacccg gtcgtagtcg tagcaacaac cgtacaaggt caactgccct tccgactcgg
     7681 cggctcgcac agtcttatcc cactccgcct gccagttcgt ctgggcagca gccaagcccg
     7741 cggttgcagc gagcaaaacc gcaacaacga aaaacaatcg cggcgcaaat ttcataacgt
     7801 gaccaaacca aacgggtttc gcgtgcgtga tcgaaaccgg gcgaacacct gaccccatac
     7861 gcctaaccct tgacatcgtt taacggctcc gcagatctca ttcaa
//
LOCUS       DASQKS010000579         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5773007, whole genome shotgun sequence.
ACCESSION   DASQKS010000579 DASQKS010000000
VERSION     DASQKS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5773007"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VE170_14775"
     CDS             complement(<1..821)
                     /locus_tag="VE170_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16758.1"
                     /translation="MTQSNAQAAALHIATYSGWYRFAARNGSWEKTHKALSFWKLTSL
                     QVDPEDAGHVYVGTEHSGLFVSRDGGAQWQRPKPNVPRLTTNALLALPGAILAGTWPA
                     ALYRARNGSAWQELDGVRLGATGGSFPPSPELSARVRFLAADNQTPRRLFAAIEVGGM
                     LVSDDDGAHWSQVTAGLDDPDVHQILPSARHPGVVLAACGEGVYRSTDRGEHWQKITP
                     AGARTFGSSVSEDGNGTLYLGVALDRPNTWLRQQRANAALFVSADGGVEWHTAIEG"
     gene            complement(902..>2142)
                     /gene="thrC"
                     /locus_tag="VE170_14780"
     CDS             complement(902..>2142)
                     /gene="thrC"
                     /locus_tag="VE170_14780"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYJ16759.1"
                     /translation="LIELRCPKCSTVTASLTEFRCSACYSVREAVVAIEHLSRDDFAR
                     LRQSRDLSIWRWFDFFPLASRASIVSLGEGSTPLLPAPGLGARIGVAKLFLKNDTVLP
                     TGSLKDRSNSVGMSVGKELGFTTASVISTGNAAASVAAYAAAAGMKSVVMVPAGTATS
                     KIIQAHAYGATVIVIAGRFDYEVADLYKNAVTEFGWYDCLSSNPYRVGKKSYAYELVD
                     QLDGMPVDWVIHPTAGGTGLVCMWQGFKELRALGWIDHAPKLVAAQSAAAAPFVAAMA
                     KQSMTIEAVEASATVAESIQVGNPSSLGDRALAALCQSQGAAVALSDGEILEAQSQIA
                     RLAGIFAEPAAATSVAAAKKMRASGAIQPDDIVVCNLTGHGLKQPEAIQISEKEFAPL
                     APSLVALRERLRQSTDAMPT"
BASE COUNT          432 a          687 c          624 g          399 t
ORIGIN      
        1 ccttcgatgg ccgtatgcca ttcgacaccg ccatcggcgc tgacaaacag ggcggcgttg
       61 gcgcgctgct ggcgcagcca ggtgttaggc cgatcgagcg cgacgccaag ataaagcgtg
      121 ccgttgccgt cttcgctgac cgaggagcca aacgtgcggg cgcccgctgg cgtgatcttt
      181 tgccaatgct caccgcgatc ggtgctgcga taaacccctt caccacaggc agcgaggacc
      241 acgccgggat gtcgcgcgct gggcaaaatc tgatgcacgt cgggatcatc cagccctgcc
      301 gtgacctggg accaatgcgc cccatcgtcg tcgctcacca acatgccgcc cacttcgatg
      361 gcggcaaaca gtcgccgcgg ggtctgatta tccgcggcga gaaaacgcac ccgcgcgctc
      421 agctcagggc tcggcggaaa actgccgcct gtcgccccca agcgtacgcc gtcgagctcc
      481 tgccatgcgc tgccgttgcg cgcgcgatag agcgccgccg gccaggtacc ggctaggatc
      541 gccccgggca gcgccagcaa cgcattggtc gtcaaacgcg gcacattagg cttcgggcgc
      601 tgccattgcg cgccgccatc gcggctgaca aaaagcccgg aatgttcggt gccgacataa
      661 acgtgaccgg catcttccgg gtcgacctgc aaggatgtca atttccagaa cgacagcgcc
      721 ttatgggtct tctcccagct gccattgcgc gctgcaaagc gataccagcc tgagtaggtc
      781 gcgatatgca gcgcggctgc ttgagcgttc gattgagtca tgcgcacacc tccacgggga
      841 gccgagtgat ttgatcccgt aatgttgttt atgcgaagtt attgcaggtc gcaggttgct
      901 gtcaagttgg cattgcgtcg gttgattgac gcaagcgctc gcgcaatgcg accaagctgg
      961 gcgcgagcgg cgcgaactct ttttccgaga tttgaatcgc ctccggctgt ttcaatccgt
     1021 ggccagtgag attgcacacc acgatatcgt ccggctggat cgccccggag gcgcgcattt
     1081 tcttcgccgc agccactgaa gtcgccgccg ccggctcggc gaagatgccg gccagccggg
     1141 cgatttgtga ctgcgcttcg aggatttcac cgtcgctcag cgccaccgcc gcgccctgtg
     1201 attggcacaa cgccgcaagg gcgcggtcgc cgagagagct gggattgccg acttgaatac
     1261 tttcggcaac cgttgcactg gcctccacgg cctcaatcgt catactctgt tttgccatag
     1321 cggcgacgaa cggcgctgcc gccgcgcttt gcgccgcaac cagtttgggc gcatgatcga
     1381 tccatcctag agccctgagt tctttgaaac cctgccacat gcaaaccagc ccggtgccac
     1441 cggcggtggg atggatcacc cagtccacgg gcatgccatc caattggtcg accagttcgt
     1501 aagcgtaact cttcttgcca acccggtaag gattggagga aagacaatcg taccagccga
     1561 actccgtcac cgcgtttttg tacagatcag ccacttcata gtcgaagcgg ccggcgataa
     1621 cgatcaccgt cgcaccgtaa gcatgggctt gaataatttt ggaagtcgcc gtgcccgcgg
     1681 gcaccatgac gacacttttc atgccggccg cggctgcata ggccgccacc gaagctgcgg
     1741 cgttgccggt tgagatcacg ctggcggtag tgaagccaag ctccttaccc accgacatgc
     1801 caacgctgtt gctgcgatcc ttaagcgaac cggtcggcag cacggtatcg ttttttaaaa
     1861 aaagcttggc cacaccgatg cgggcgccca agcccggggc aggaagcagc ggcgtgctgc
     1921 cctctcccaa agaaacaatc gacgcgcggc tcgccagcgg aaaaaagtcg aaccaacgcc
     1981 atatcgacag atcgcggctc tgccgcaagc gcgcgaaatc atctcgactc agatgttcga
     2041 tagccaccac cgcttcgcgc accgaataac aggccgaaca acgaaactcc gtcagcgatg
     2101 ccgtgacagt ggaacatttc ggacaacgca attcgatcag tt
//
LOCUS       DASQKS010000580         8506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_750205, whole genome shotgun sequence.
ACCESSION   DASQKS010000580 DASQKS010000000
VERSION     DASQKS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750205"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VE170_14785"
     CDS             complement(<1..557)
                     /locus_tag="VE170_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYJ16760.1"
                     /translation="MKFKKTIITVVLLLAAGGAGYAYWRMGNGPKEPPYLTTPVAKAN
                     IRQVVSSTGTLQAVTTVLVGSQVSGTIAKLNADFNSKVKKGEIVAELDQARFAARVEE
                     TRANVAAAQANLAKSKVSLEDAERTLKRTKELKQRELVSQSELDAAQTAYDSARSQVS
                     VTQAQVGQAQASMNQAQIDLGYTVIR"
     gene            complement(554..1030)
                     /locus_tag="VE170_14790"
     CDS             complement(554..1030)
                     /locus_tag="VE170_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16761.1"
                     /translation="MSQLLGKELPAALQQRLGGSEVESHEGKIIPIFTIDEAGWAHPA
                     LLSYYEIAAKDACTLDMALWKDSSTSKNLRQAGKVTVMISDSGVNYYLKGSVRELHYE
                     MPGAAPVSRFRVTLEQVIEDQEPNAEITTGLTYRRMTKRDPNDFAAKVFRLLREES"
     gene            complement(1062..1535)
                     /locus_tag="VE170_14795"
     CDS             complement(1062..1535)
                     /locus_tag="VE170_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ16762.1"
                     /translation="MAEDLKQQIIDYLRSHNTMTLATCAGDAPWAATVFFASDDLRLY
                     FFSAPESRHCQNLAANGRVAVTVQEDYQDWRKIKGVQLEGRVELVDGVVAKGKAMAIY
                     AFKYPEVIKLFTDPTSGVFHKAFLKVKFYCVVPEKLFFIDNAQGFGKRQELLLEP"
     gene            complement(1596..2645)
                     /gene="fni"
                     /locus_tag="VE170_14800"
     CDS             complement(1596..2645)
                     /gene="fni"
                     /locus_tag="VE170_14800"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017320956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HYJ16763.1"
                     /translation="MPRPRPSKALSQTQARKKQHLEICLDTASVSGYSSTGFDRYRFI
                     HNALPELDLDEIDVATTFLGKKLKAPILISSMTGGFDLALKVNRNLAAAAQQLGIAMG
                     VGSQRVAIEESSAAASFQVRDVAPDILLFGNLGAVQLNYGYNIEQCRRAVAMIGADAL
                     ILHLNVLQEALQPEGNRNFKGLTKKIAVVCRELKVPVIVKEVGNGISVEVAKRLDSAG
                     VAAIDVAGKGGTAWSAVEAQRASQQGKAYSATFTDWGIPTEEALVAVRAALPEIQLVA
                     SGGIRSGLDVAKSIALGADLAAFGQPLLAAALESPGEVVKFIGEIIQELRVAMLCVGA
                     ANLESLRRSALVRSA"
     gene            complement(2666..3325)
                     /locus_tag="VE170_14805"
     CDS             complement(2666..3325)
                     /locus_tag="VE170_14805"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003854263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HYJ16764.1"
                     /translation="MNTKKRLIIGVSGATGAIYGVRMLEILSKIDDIETHLVLSKAGK
                     MTIQVETPYSVKDVEAMADVVHDVNNVGASISSGSFRTAGMVIAPCSMKSMGAIAHSL
                     GGDLLVRAADVVLKERKKLLLVVRETPLHLGHLEAMATLTRMGAIIFPPVPAFYHRPK
                     TLDDVINQTVTRILDQFDIDVELFHRWDDAGMSRHPDAAKSKPTPISAAKERKEAKKQ
                     R"
     gene            complement(3322..3729)
                     /locus_tag="VE170_14810"
     CDS             complement(3322..3729)
                     /locus_tag="VE170_14810"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HYJ16765.1"
                     /translation="MERLSGIIFSDLGQASSFMALDWVQDLLNERLGYHPFPATLNVR
                     PKGAEDAQIWRRVQRDHAGTPLTPASDGHCGAKLYRVEVYQEANSTKVGGAILLPEVD
                     NYPQDKIEIVAPVRLKDHFRINDGDQLTVEFLS"
     gene            complement(3751..4524)
                     /locus_tag="VE170_14815"
     CDS             complement(3751..4524)
                     /locus_tag="VE170_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HYJ16766.1"
                     /translation="MSSLSAELVKIAEQGEAAVLVTVVEVTGDGQIQPGAKCLIRDGM
                     AQVETIGDAKVVAALVRESDSHARAEKSQMVSLTIPDSTIKLEVFFEVMPAPPRLIVV
                     GAGHIAVPLVRIAKVLDFHVTVIDDRLLYANRERFPDADEVLTGDMAQMLKEMTLTPS
                     CYIVLITRGHAYDEPCLRNIIHSKAKYIGMIGSRRRIKACFQRFRDEEKVTEEAIERI
                     YAPIGLDIATETPAEIALSILGEVIKIRRGGKAASLSGH"
     gene            complement(4521..4916)
                     /locus_tag="VE170_14820"
     CDS             complement(4521..4916)
                     /locus_tag="VE170_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYJ16767.1"
                     /translation="MAGPGACYTDEDLEAKKKETIYHQVNEFLAKGETVAVATIVSTK
                     GSTPREVGAKMVVTAWGEILGTIGGGCGEADVKREAIEVIRSRKPRTVRVDLLDDISS
                     DSPAVCGGIMNVFIDPWWQERDQEAVSGK"
     gene            complement(4989..6203)
                     /locus_tag="VE170_14825"
     CDS             complement(4989..6203)
                     /locus_tag="VE170_14825"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ16768.1"
                     /translation="MDITPETVAEAVNKVASVRGKGTLQNMMAFGRALKHLGVKVSLS
                     QILDASRSVDLVDLSARGDFRALLRANLILQKEDFPVFDMLFDCFWREQSYERVPMET
                     MDIQGTPTESNAPEGGDEEGGVEEAASEAIANENLPLENLDEFAVPTYSAQELMNRKD
                     FSEMGTEESRAIARAILLIATKIATQISRRKKIGRKGPVIDPRATMRRSMKYGGEVIE
                     MAHRKRRIKKTKVVLLCDVSGSMDCYSRFLIQFMYGLQNELWGVETFVFSTSLSRITH
                     LIRTKNIVDALDKISGTILGWSGGTNIGRSLHTFNRNFAPSMVTHRTVVVIISDGWDR
                     GDVGLLEKEMQDIKRRAKKIIWLNPLLASENYEPLCKGMQAALPYLDMFLSVHNVNSL
                     VALGRTLEKMVA"
     gene            complement(6210..7130)
                     /locus_tag="VE170_14830"
     CDS             complement(6210..7130)
                     /locus_tag="VE170_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYJ16769.1"
                     /translation="MDPKQLTIEQVQEILLTEKYVCDRSLATVVYLSLVMGKPLLLEG
                     EAGVGKTEIAKVLSKVLGARLIRLQCYEGLDASTALYEWNYPKQMLHIKLEEIERGDK
                     KKVETEIFTQDYLVKRPLLEAIQSEDANPPVLLIDEIDRADMEFEAFLLEVLSDFQIT
                     IPELGTVTAKHRPYVFLTSNRTREIHDALKRRCLYHWIEYPTFDKEYEIITTKFPEVE
                     TRMAQQICAFMQKVREMNFYKRPGVAETLDWASALIALNRKQLDDKSVIETMGCVFKY
                     REDLSHLREQVENRQFSLDALLRTSPELVS"
     gene            complement(7582..>8506)
                     /locus_tag="VE170_14835"
     CDS             complement(7582..>8506)
                     /locus_tag="VE170_14835"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYJ16770.1"
                     /translation="FNTQDAETTAVVQDGETLVIGGLIGELKRKGRSGIPYLMDIPVI
                     GRFFGTTNEATDRTELIMLITPRVIRNREEAKIVTSDFKAGLEKVRAELERLMNERER
                     LLPPRPLPPLPDPNQYYQYDQQQPEPPTPPVGPRAALPAFIGNVTLLSNDPLTGRMMP
                     PMAVPQSAQPVWLAQNYQPAVPANTQPKPSVAPMPPEQVLSFIQAVPVAAPAPPPAAV
                     KLVQPQKPTVGGRTWTVQVAAIAESSGAESLAQKLRRMGYHAYVRVTHGNYKVWHRVR
                     VGQLESVRDAAELRRTLMAQNQYKDAYIAAY"
BASE COUNT         1728 a         2525 c         2330 g         1923 t
ORIGIN      
        1 cgaatgaccg tgtagcccaa atcgatctgc gcctgattca tcgacgcctg agcctgtccc
       61 acctgtgctt gagtaacgct gacctgggaa cgcgccgaat cgtaggcggt ctgcgccgcg
      121 tctaattcgc tctgcgatac cagctcgcgt tgtttgagtt ctttggtgcg tttcaaagtc
      181 cgttcggcat cttcgaggga gactttcgac ttcgccaggt tggcttgagc ggccgccaca
      241 ttggcgcggg tttcttccac ccgggcggca aagcgcgcct ggtcgagctc ggcgacgatc
      301 tcgccttttt tgaccttgct attgaagtcg gcgttgagct tggcaatggt gccggatacc
      361 tggctgccga ccaacacggt ggtcaccgcc tgcagcgtac cagtcgaaga aaccacttgg
      421 cgaatgttcg ccttcgccac cggggtagtt aaatacggcg gctccttggg cccattcccc
      481 atacgccaat aagcgtatcc ggcgccgccg gcggccaaga gcaaaaccac cgtgattatg
      541 gtttttttaa atttcatgac tcctcgcgca gcaggcgaaa tacctttgcg gcaaaatcat
      601 tgggatcgcg cttggtcatg cgccgatacg tcaaccctgt ggtgatctcg gcattgggct
      661 cttggtcctc gatgacctgc tccaaagtca cgcgaaatcg cgacactggc gccgcccccg
      721 gcatctcgta atggagctcc cgcacactgc ccttgagata ataattcacc ccactgtcgg
      781 agatcatcac cgtcactttg ccggcttggc gcaaattctt cgacgtcgag ctgtccttcc
      841 acaacgccat gtcgagggta caagcatcct tggcggcgat ttcgtaatag gacagcagcg
      901 ccggatgggc ccagccggcc tcgtcgatgg taaaaatcgg aatgattttg ccttcgtggc
      961 tttcgacctc gctaccgccg aggcgctgct gcaatgccgc cggtaattcc ttaccaagta
     1021 gctgtgacat ggtgaatcga tactccttag atgtttagac gctatggctc caagagcagt
     1081 tcctggcgct tgccaaaccc ctgagcgttg tcgataaaga acagtttctc gggcaccacg
     1141 caatagaact tgactttcag gaatgccttg tgaaatacgc cgcttgtcgg atcggtaaag
     1201 agcttgatga cttccgggta tttgaaggca taaatggcca tcgctttacc ctttgccacg
     1261 accccgtcga cgagctcgac ccgcccttca agttgaacgc ctttgatctt gcgccagtcc
     1321 tgatagtctt cctggaccgt cacggctacc cgcccgttgg ccgccaaatt ctggcagtgg
     1381 cgcgattccg gcgccgaaaa gaaatacagc cgcaaatcat cgctggcaaa aaacaccgtg
     1441 gccgcccaag gcgcgtcgcc ggcgcaggtc gccagcgtca tagtgttatg acttctcaag
     1501 taatcgatta tctgttgttt gagatcttcg gccatcgacg caggggaatc tacgagaatc
     1561 gccgcctgac aacaagcgag ggcgcagtgc cggcgtcaag cggagcggac caaggccgag
     1621 cggcgcaacg actccaggtt cgccgcgccg acacaaagca ttgcgaccct gagttcctga
     1681 ataatttcac cgatgaattt taccacttcg ccgggcgact ccaaagccgc agcgagcagt
     1741 ggctggccga aggccgccag atcggcgccc agggcgatgc ttttggccac gtcgagaccg
     1801 ctgcgaatgc cgcccgaggc gaccaattga atttccggca aagcagcccg tacagcaacc
     1861 aaggcttcct cggtcggtat tccccagtcg gtgaaagttg cgctgtaggc cttgccctgt
     1921 tggctcgcgc gctgggcttc aacggcggac caggcggtgc ctcctttgcc ggcgacatcg
     1981 atggccgcca cgccagcgct atctaaccgt ttggccacct cgaccgaaat accgttgccg
     2041 acttctttga cgatgacagg aactttcaac tcgcggcaaa ccacggcgat ctttttcgtc
     2101 aatcccttga agttgcggtt gccctcgggc tgcagcgctt cctgcagcac attcaaatgc
     2161 agaatcaaag cgtcggcgcc gatcatcgca acagcgcgcc ggcactgctc gatgttgtag
     2221 ccataattca actgaaccgc gcccaagttg ccaaacaata agatatccgg cgccacatcg
     2281 cgcacttgaa acgatgcggc ggcggacgac tcttcaatgg cgacgcgctg agacccgacc
     2341 cccatggcga tgccgagttg ctgcgccgcc gcggcgagat tgcgattgac cttgagcgcc
     2401 agatcaaaac cgccggtcat cgacgaaatt aagatcggtg ctttgagttt cttaccgagg
     2461 aatgtcgtcg cgacatcgat ctcgtcgaga tccagttcag gcaaagcgtt gtgaatgaaa
     2521 cgatagcggt cgaatccggt gctggaatag ccactgacac tggcggtgtc gaggcaaatt
     2581 tctagatgct gttttttgcg cgcttgggtt tggctgaggg ctttgctggg ccgcgggcgt
     2641 ggcatagttc agagagccgc caaggctagc gctgcttctt cgcttccttg cgctctttgg
     2701 ccgcgctgat cggcgtcggt ttggacttcg ccgcgtcggg atggcggctc atgccggcgt
     2761 cgtcccagcg gtgaaacagt tcgacatcga tgtcgaactg atcgagtatc cgtgtcaccg
     2821 tctggttgat cacgtcgtcc aaagtcttcg gccggtggta aaacgccggc accggcggga
     2881 agatgatcgc gcccatgcgc gtgagcgtcg ccatcgcttc gagatggccc aaatgaagcg
     2941 gcgtttcgcg aaccaccagc aagagcttct tccgttcttt caaaaccacg tcagcggcgc
     3001 gcaccaacaa atcaccgccc agcgaatgag cgatcgcgcc catggacttc atcgagcaag
     3061 gcgcgatcac catgcccgcg gtgcgaaaag aaccgctgga aatgctcgcg ccgacgttat
     3121 taacgtcatg caccacgtcg gccatcgctt cgacatcttt gaccgagtag ggcgtttcta
     3181 cttggatcgt catcttgccg gccttgctca agaccagatg agtctcgata tcatcaatct
     3241 tggagagaat ctccaacatg cgcacgccat agatcgcccc ggtggcgccg gagacgccga
     3301 taatgagccg cttcttggta ttcaactgag aaactccact gtaagttgat cgccgtcgtt
     3361 aatccggaaa tgatctttca ggcgcaccgg cgcgacaatt tcaattttat cctgcggata
     3421 gttatccact tcgggaagca agatcgcgcc accgaccttg gtgctgttgg cctcttgata
     3481 aacttcgacg cggtataact tcgcgccgca atgaccgtcc gatgccggcg tcaagggcgt
     3541 tccggcatga tcacgctgca cccgccgcca aatctgggcg tcttcggcgc cttttggccg
     3601 gacattgagg gtcgctggaa atggatgata gcccaggcgc tcgtttaaca aatcctgcac
     3661 ccaatcgagc gccataaacg atgacgcttg tcctaagtcg gaaaaaatga ttccgctcaa
     3721 tctttccatc gttgtcgcgc gcgcaatatc ttagtggccc gacagagatg ccgctttgcc
     3781 gccgcggcga atcttgatca cctcgccgag tatcgacaaa gcgatttccg ccggcgtctc
     3841 ggtggcgata tcgagaccga tgggcgcgta gatgcgttcg atcgcctcct cggtgacttt
     3901 ctcctcatcg cggaaacgct gaaaacacgc cttgatgcgc cgccggctgc cgatcatgcc
     3961 gatgtacttg gctttgctgt ggatgatatt gcgcagacag ggctcgtcgt aagcatggcc
     4021 gcgggtaatc aagacgatat agcaggacgg cgtcagggtc atttccttga gcatctgcgc
     4081 catgtcgccg gtcaacactt catcggcatc ggggaaccgc tcgcggttgg cgtagagcaa
     4141 ccggtcatcg atcacggtga cgtgaaaatc gagcaccttg gcgatcctga ccagtggcac
     4201 ggcgatatga ccggcgccga cgacgatgag cctgggcggc gccggcatta cttcgaaaaa
     4261 tacttccagc ttgattgtgc tatccggaat cgtcagcgaa accatctgcg acttttccgc
     4321 ccgcgcatgg ctgtcgctct cgcgcaccag ggcagccacc actttggcgt cgccgatggt
     4381 ttccacctgc gccatgccgt cgcggatcag acacttggcg ccgggttgga tttgcccgtc
     4441 gccggtgact tcgacgaccg tgaccagcac cgccgcttcg ccctgctcgg cgatcttgac
     4501 taactccgcg gacagcgaac tcatttgccg cttaccgcct cctgatcgcg ttcctgccac
     4561 caaggatcga tgaagacatt catgataccg ccgcataccg ccgggctatc cgaggagata
     4621 tcgtcgagta aatcgacgcg caccgtgcgc ggcttgcgcg agcggatcac ttcaatagct
     4681 tcgcgtttaa cgtcggcctc gccgcaaccg ccgccgatgg tgcccaaaat ttcgccccaa
     4741 gcggttacta ccattttggc gccgacttcg cgcggcgtgg agcccttggt cgagacgatg
     4801 gtcgccaccg cgacggtctc gcctttggcg agaaactcgt tgacctgatg atagatcgtt
     4861 tctttttttt tcgcttctaa atcctcatcc gtataacagg cgccgggtcc agccatgttc
     4921 gcacctccta agatcgccaa gttggaagat cgaggattgc catcctctat ccccgacctc
     4981 atggttaatc atgccaccat tttctcgagc gttcgtccca acgcaaccaa actattgaca
     5041 ttgtgcaccg acaaaaacat atccaaatac ggcagcgccg cttgcatgcc tttgcacagc
     5101 ggttcgtaat tctcgctcgc cagcaaaggg ttgagccaga tgattttctt ggcgcgtcgt
     5161 ttgatgtcct gcatttcttt ttccaacaaa ccgacgtcgc cgcggtccca gccgtcgctg
     5221 ataatcacca ccaccgtgcg gtgggtcacc atgctcggcg cgaaattgcg attgaaggtg
     5281 tgcagcgaac gaccaatgtt ggtgccgccg gaccagccaa gaattgtgcc ggaaatcttg
     5341 tccaaagcat cgacaatgtt cttggtgcga atcagatggg taatccggct gagcgatgtg
     5401 ctaaaaacga aagtctcgac gccccacagt tcgttttgca agccgtacat gaactgaatc
     5461 agaaaccggc tgtaacaatc catcgagccg ctgacgtcgc acaggagcac gactttggtt
     5521 ttcttgatgc gccgcttgcg atgcgccatc tcgatcactt cgccgccgta tttcatgctg
     5581 cgccgcatgg tcgcgcgcgg atcgatcacc ggacctttgc ggccgatttt cttgcgccgg
     5641 ctgatctgag tggcgatctt ggtggcgatc agcagtatgg ctctggcgat agcgcggctt
     5701 tcctcggtgc ccatctcgct gaagtccttg cggttcatca gctcctgggc gctataggtc
     5761 ggcacggcga actcatcgag attttccagc ggcagattct cattggcgat cgcctcggag
     5821 gcggcttctt ccacgccgcc ttcttcgtcg ccaccctccg gggcgttgga ttcggtgggc
     5881 gttccttgga tatccatcgt ctccatcggc acgcgctcat aactctgctc gcgccagaaa
     5941 caatcgaaga gcatgtcgaa caccggaaag tcttcttttt gtaaaatcaa gttggcgcgt
     6001 aacagcgcac ggaagtcgcc gcgcgcggag aggtcgacca aatccaccga acgagaggca
     6061 tcaaggatct ggcttaagct gaccttgacg cctagatgtt tcaaggcgcg gccaaatgcc
     6121 atcatgttct gcaaggtgcc cttgccgcgc accgaggcga ctttattgac cgcctccgca
     6181 actgtttcgg gggtgatatc catcgatctt taactgacta gctccggcga ggtgcgcagc
     6241 agcgcatcga gactgaactg gcggttttcc acctgctcac gcaggtggct caaatcttcg
     6301 cggtatttga acacgcagcc catggtctcg attaccgact tatcatccaa ttgcttgcgg
     6361 ttgagcgcaa tcagcgccga cgcccaatcc aaagtctcgg cgacgcccgg gcgtttatag
     6421 aaattcattt cgcgcacctt ttgcatgaaa gcgcagattt gctgcgccat ccgggtttcg
     6481 acttcgggaa acttggtggt gatgatctcg tattccttgt cgaaggtcgg atactcgatc
     6541 cagtggtata agcagcgacg tttcaaggcg tcgtggatct cccgcgtacg gttggaggtg
     6601 agaaacacat agggccgatg cttcgccgtc accgtcccca actcgggaat ggtgatctgg
     6661 aaatcggaca agacttcgag caggaacgcc tcgaactcca tatcggcgcg gtcgatctcg
     6721 tcgatcaaca gcaccggcgg attggcatcc tcgctttgga tcgcttcgag cagcggccgt
     6781 ttgaccaagt agtcctgggt gaaaatctcg gtttcgactt tctttttatc gccgcgctcg
     6841 atctcttcga gcttaatgtg gagcatctgc ttagggtagt tccactcgta tagggcggtg
     6901 ctggcgtcga gcccttcgta gcactgcagc cggatcaacc gggcgcccag gactttggac
     6961 aataccttag cgatttcagt cttaccgacg cccgcctcgc cttcgagcag tagcggtttg
     7021 cccatgacta gcgataaata gaccacggtc gcgagggaac ggtcgcagac atacttttcc
     7081 gtgagaagaa tttcttgaac ttgttcgatg gtcagctgtt tcggatccac gcttgattca
     7141 tcctttgcag ttaattgttg cagggccggt caggcggaat tttagactgc gatacgagtg
     7201 taagagagcc tcgagaaccc tccgccaacg gccctagtct tgtcggaaat cgtgtcaaca
     7261 tacccccttt cacccgttgg atcaacgtcg ccgagctaca tactagcccg tgtttctaca
     7321 gcgtcatgag catgccgcgg atcagcccgg cgatcgccga ccgcaccggc tcacgggcga
     7381 tttggccaac gatttccccg cccagccatg ggtctatgat ttatctaaat cataacaaag
     7441 cgcattgcgc gatcaaagtc gacgaaaatg acagttcaca tggggatact caagtccatc
     7501 gacagccgcc cgcgcgcggg caaaaaaaag ccgcccgatg agcttgaccc acgggcggct
     7561 aatcggcaaa aagcctcgcg actagtaagc cgctatatag gcatccttgt attggttttg
     7621 agccatcagt gttctgcgca gttccgcggc gtctctaacg ctttccaatt gacctacgcg
     7681 cacgcggtgc cacactttgt aatttccatg ggtgactctc acgtaggcgt ggtaacccat
     7741 gcggcggagt ttttgcgcta acgattcagc cccgctactt tcggctattg ccgctacttg
     7801 cactgtccat gtccgtccgc cgaccgtggg cttttgcggc tgcaccagtt taacggcagc
     7861 cggcggtggc gctggcgccg caactggcac ggcttgaata aatgaaagta cctgctccgg
     7921 cggcattggt gcgacgcttg gctttggctg ggtattagcc ggcactgcag gctggtaatt
     7981 ctgcgccagc cataccggtt gagccgattg cggcacagcc attggcggca tcatccggcc
     8041 tgtgagtgga tcgttcgata gtaaagtgac attgccaata aacgccggca acgcggcgcg
     8101 cggcccaacc ggtggcgtgg gcggctccgg ctgctgctga tcgtattggt aatactgatt
     8161 cggatccggc aacggcggta gtggacgcgg tggcaagagt cgctcgcgtt cgttcataag
     8221 tcgttcgagt tccgcccgta ccttctcgag cccagctttg aagtcgctcg tgacgatctt
     8281 ggcttcctcg cgattacgaa tcactcgcgg cgtgatcaac atgatcagtt cggtgcgatc
     8341 ggtggcctca ttggtcgtgc caaaaaatcg cccgataaca ggaatatcca tcaaatacgg
     8401 gatgcccgac cggcccttgc gtttcagctc gccgatcaag ccaccgatca ctaaagtctc
     8461 gccatcttga accactgccg tggtttccgc gtcttgagtg ttaaaa
//
LOCUS       DASQKS010000581         5381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5776993, whole genome shotgun sequence.
ACCESSION   DASQKS010000581 DASQKS010000000
VERSION     DASQKS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5776993"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VE170_14840"
     CDS             complement(<1..1086)
                     /locus_tag="VE170_14840"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16771.1"
                     /translation="MKAESMFKVRREDLHNFTREENETLTRVGPATAMGNLFRQYWLP
                     VVPSAHLSEAGGAPLRVKLLGEDLVLFRTHANQVGLIGAYCPHRLAPLYFGRVEQDGL
                     RCPYHAWKYSPAGKCIEMPNIPPAQQFIDEVEHAGYPCVERGGIIWTYMGLAKDLPEL
                     PDLEFLRVADDLRIYRLFFQEANYLQVLEGGIDPTHVMWLHSPYDLNDEEMNAQQPAQ
                     QRAANKLGVKKTPEDVELVEHDAGFTYGARRSLSGGKSLWRVNQFIMPWYTMPPGGDQ
                     RAARAFIPVDDENCVKWQVRWYPTTEIAARTEEGVRAFFDEEAYDPPSNAMPFGHIRM
                     KAKRANDYLINWDLHKSRRIGIAGVNLQ"
     gene            1240..2235
                     /locus_tag="VE170_14845"
     CDS             1240..2235
                     /locus_tag="VE170_14845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16772.1"
                     /translation="MRYWFWLPFLLLCLATADGQERKLERFILSNSTLSESRAPLYIA
                     QDLKLFEKFGLDAQIVNIRGSAINNAALMAGEIQMAVANGTIAITAAARGAPIVIVGT
                     IGPTRYSLISRALTSAQQLKGKTVGIGGFGIGDYFVVRRLLPKLGLNPDKDVALLPTG
                     TTSSFERINMMLSGKADAVMATKSNIDRVEMRGIKLNVLAGTEELGVEVSGGDFFVTR
                     EFLRNKPQLVKAVFRAFGEAVRIGRQNKEVFHRAIRRYMKEDNPVLIDKFYDAHYYFA
                     AKPRTMFPLEAALDLDIRDMQATVAELRGRRAAEFIDTTALREVEKEGFFNWAKN"
     gene            2386..3450
                     /locus_tag="VE170_14850"
     CDS             2386..3450
                     /locus_tag="VE170_14850"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16773.1"
                     /translation="MKLRVGNGCWHAGHVQAAMNALEAGFFAQEGLDVEIVHAKINPK
                     GIESSRPDGERYDEVGTVLRDMIAFNIDIIPDVHVRTPFAERALGNDELRIIGGWRNW
                     FPATLVTAPGIKSIKELKGKRIGDWYKGGIATMWIEHQLRQAGLNPATDIEWKVGYRY
                     GSMREAWKPLHAGETDAALVRNQFVPELLEKGFNKLYDFVEDSKPHGRPDRVTVARKS
                     FIARNPELIKRYWKATIRGYHFMRIVPENFPFQRMVESKIRVNNPDPSENMRDLRPIR
                     IMESAFFPLDGQLTVEGVWRILEEHQDAGVLSKAITRKDVEEVVSQNLVKEAWEEISQ
                     TDEVKKNLERLQPVVEKYGY"
     gene            3625..>5381
                     /locus_tag="VE170_14855"
     CDS             3625..>5381
                     /locus_tag="VE170_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16774.1"
                     /translation="MTETNIPAELEKFAERLRLYAKALSGREIHLAPRAARAGRGAGW
                     RTPHQDDQTIVIELPAKIDRFPAEKDNFNWYKVILTHQAGHVEFGTFDFVFAKPARQF
                     DDWRSQLAAGKPLIESADEFGTFWQLFPDIDLAAAIFELVEDSRIDAHVLAAYPGIRP
                     LYQRVANSVRAGRPRLALLPLREGFLEALVQLGLGGDPLAVAPLAIKSEIKLGAVLIA
                     KVRSPHASVEDSAETTLRLYELAANLPNRPAYDWDDDCVHDRHQQRPPREQAPDALSE
                     PVSGNDDMPFAPPQEVEFRFDSDGQRLRRFKSKPRGDEAPPVLTADTRAAGPLTRDQP
                     FTYLYPEWDFRGGAYRQRWCRVRERVMDQGSADFYMEALAEHRPLVGQVVNRIEHLIP
                     ELFCKVTRRDDGEDVDLDAVIERFVDRRTGGMPSEQLYWRRERTQRDVAVAVLLDMSA
                     TTDEFVQLEAAKAHRPTGPSAQAYSDYLKRLAAGVDQRGKPLRRRTIDIEKQAAIVLC
                     QALEKIGDSYAVYAFSGSGRAEVEFHVVKDFHERLTQRIGRRIDRIQPAHGTRMGAAI
                     RHALGKLERVEANTRLLFL"
BASE COUNT         1121 a         1466 c         1647 g         1147 t
ORIGIN      
        1 ctggagattg acgccggcga tgccaatgcg ccggcttttg tgcagatccc aattgattag
       61 atagtcattg gcccgtttgg ccttcatgcg aatatggcca aagggcatgg cattagacgg
      121 cggatcgtag gcttcttcgt cgaagaaggc gcgcacgcct tcctcggtcc tggctgcgat
      181 ctccgtcgtg ggataccagc gcacttgcca tttgacgcaa ttttcatcgt ccaccggaat
      241 gaaagcgcgg gccgcccgct gatcgccacc cggcggcata gtgtaccagg gcataatgaa
      301 ctgattgacc cgccacaagc tcttgccgcc gctgagcgaa cgtctcgcgc cgtaggtaaa
      361 tccggcgtca tgctcgacca gttcgacatc ttccggggtt tttttgacgc cgagtttgtt
      421 ggcggcgcgt tgctgcgccg gttgttgagc attcatctct tcgtcgttga gatcgtaggg
      481 cgagtgcagc cacatgacgt gggtcggatc gatgccgcct tcgaggactt gcaaataatt
      541 ggcttcctgg aaaaacaacc ggtagatccg caagtcgtcg gcgacgcgca gaaactcgag
      601 atccggcagc tcgggcaaat ccttggcgag ccccatatag gtccagatga tgccgccgcg
      661 ctcaacgcag ggataaccgg catgctcgac ttcgtcgatg aactgctggg ccggcgggat
      721 attgggcatt tcaatacact tccccgccgg cgaatatttc caagcatgat aaggacagcg
      781 caggccgtcc tgttcgaccc ggccgaaata gagcggcgcc agccgatgcg gacagtaggc
      841 gccgatcaag ccgacctggt tggcgtgggt gcggaacaga actaaatctt cgccgagcag
      901 cttgacccgc aggggcgcgc cgccggcttc gcttagatgt gccgaaggca ccaccggcag
      961 ccagtattgg cgaaacaaat tgcccatggc cgtcgccggc ccgacccgcg taagggtttc
     1021 gttttcttcg cgcgtgaagt tgtgcaaatc ttcacgccgc accttgaaca tgctttcggc
     1081 tttcacggtt tggttcccct tggtgagttt gttttggttt gtatcacagg tgatgctact
     1141 tgcaacggtt atgtcgcagg tgtgcagcat gaataaatcg gccctctttt gccgacctgg
     1201 ctttgactcg ccagcgatct ccagataggt acaaggtcta tgcgctattg gttttggctc
     1261 ccgtttcttc tcctttgtct ggcaaccgcg gacggccagg aaagaaaact ggagcggttt
     1321 attctttcta actcgacgct atcggagagc agggcgccgc tttatattgc ccaggatctg
     1381 aagctcttcg aaaagttcgg tctcgacgcg cagatcgtca atattcgcgg ttcggcgatc
     1441 aacaatgccg cccttatggc gggtgagatt cagatggcgg tggccaatgg gaccatcgca
     1501 atcaccgcgg cggcgcgcgg cgcgcccatc gtcatcgtcg gcaccatcgg gccgacgcgc
     1561 tacagtttga tctcgcgggc gctcacctcg gcgcagcagt tgaagggcaa aaccgtcggc
     1621 atcggcggtt tcggcattgg cgattatttt gtcgtgcgcc gcttgctgcc aaaattgggg
     1681 ttgaacccgg acaaggacgt agccttgttg ccgaccggga cgacatcatc gttcgagcgc
     1741 atcaacatga tgctcagcgg caaagccgac gctgtcatgg cgacgaaaag caatatcgac
     1801 agggtcgaga tgcgcggcat caaattgaac gtgttggccg ggacggagga gttgggcgtc
     1861 gaagtttccg gcggcgattt cttcgtgacg cgggagtttt taaggaacaa gcctcagtta
     1921 gtcaaagcgg tgtttcgcgc ctttggcgag gcggtgcgca ttggccgtca gaacaaggag
     1981 gtctttcatc gcgctatccg ccgctatatg aaagaggaca acccggtgct gatcgataaa
     2041 ttttacgacg cgcactatta tttcgcggcc aagccacgca ccatgtttcc gctggaagcg
     2101 gccctcgatc tcgatattcg cgatatgcaa gcgacggtgg cggaactgcg cggccggcgc
     2161 gccgcggaat ttatcgatac gaccgcgttg cgcgaagtgg agaaggaagg ctttttcaat
     2221 tgggcgaaga attgaagcag gatcatcgca gcagcgattg ttgggtgggt ggaggcgcag
     2281 agcgccgata cttaaggttc gggacgatgg gtatcgcttg cgctccaccc atcctacgta
     2341 ctccatccta taaagtattt tatcatgagg agtcgaaact aaaacatgaa actgagagtc
     2401 ggcaacggtt gttggcatgc gggccatgtg caggcggcga tgaatgcgct ggaagcaggc
     2461 ttctttgccc aagaagggtt agacgtcgag atcgttcacg ccaagatcaa ccccaaaggc
     2521 atcgagtcga gccgcccgga cggcgagcgc tacgacgagg tgggcacggt tctacgcgac
     2581 atgattgcat tcaatatcga catcattccc gatgttcatg tgcgcacgcc cttcgccgag
     2641 cgcgccttgg gtaacgacga actgcgcatc atcggtggtt ggcgcaactg gttcccggcc
     2701 accctggtta ccgcgccggg aatcaaatcg atcaaggaat tgaagggcaa gcggattggc
     2761 gactggtaca agggcggcat cgcgaccatg tggatcgagc atcaattgcg ccaggccggt
     2821 ttgaatcccg ccaccgatat cgagtggaaa gtcggctaca ggtacggctc catgcgcgaa
     2881 gcctggaagc cgctgcacgc gggcgagacc gatgcggcgt tggtgcgcaa tcaattcgtc
     2941 cccgagcttt tggagaaagg ttttaacaag ctctatgact tcgtcgaaga ttcgaaaccc
     3001 catggccgtc cggaccgggt cacggtggcg cgcaagtcgt tcattgcgcg caatccggaa
     3061 ttgatcaagc gctactggaa ggcgaccatc cgcggttatc acttcatgcg catcgtgccg
     3121 gagaattttc cctttcagcg catggttgag tcgaagattc gcgtcaataa tcccgacccg
     3181 tcggagaaca tgcgcgatct gcggccgatc cgtatcatgg agagcgcatt ttttccgctt
     3241 gatggccaac tgacggtgga aggggtatgg cgtatcttgg aagagcatca agatgccggc
     3301 gtcttgtcca aggcgatcac gcgcaaggat gtcgaagagg ttgtcagcca gaatctagtg
     3361 aaagaagcgt gggaagaaat ttcccaaacc gatgaagtga agaaaaatct cgaacggctg
     3421 caaccggtgg tggagaagta cggctattga aacatgtaag aaaagaacaa tggccgcaaa
     3481 gaacgcaaag gggagattaa aaatcaggag tcgttggggc gagtttgaaa ttcgccttgt
     3541 tggcttcgcg cctttcccgg tgagatccgt tattttaagt gcattagtta gcttcgactt
     3601 ataacccagc tctgtctctt tcccatgact gagaccaaca tcccggcgga actcgagaaa
     3661 ttcgcggaga ggctgcggct ttacgccaag gcattaagcg gacgggagat tcacttggcg
     3721 ccgcgcgcgg cgcgcgctgg ccggggcgcc ggttggcgga caccccatca ggacgatcaa
     3781 accattgtca tcgaactgcc ggccaagatc gatcgtttcc ccgccgagaa ggataatttc
     3841 aactggtaca aggtaattct gactcatcaa gccggccatg tggaattcgg cacctttgat
     3901 tttgttttcg ccaagccggc gcgccagttt gacgattggc gcagtcagct tgccgcgggc
     3961 aaaccgttaa tcgagtcggc cgatgaattt ggcacattct ggcagctgtt tcccgatatc
     4021 gacctcgctg cggcgatttt tgagcttgtc gaagactcgc gtatcgatgc ccatgtctta
     4081 gccgcctatc caggaattcg cccgctgtac cagcgtgtcg ctaattcggt tcgcgccggg
     4141 cggccacgtt tggcgctgtt gccgttgcgc gaagggtttc ttgaagcgct ggtacaattg
     4201 ggcctgggcg gcgatccact ggccgtggcg ccattggcaa ttaaaagcga aattaaattg
     4261 ggcgcggttc tgattgccaa ggtgcgctcg ccccacgcca gcgtggagga ttccgcggaa
     4321 acgacgttgc ggctttacga gctggcggcc aacttgccca atcggccggc ttatgactgg
     4381 gacgatgact gcgtacatga tcgccaccaa cagcgcccgc cgcgcgagca ggcgccggat
     4441 gcactgagcg agccggtgtc tggcaatgac gacatgccgt ttgcaccgcc ccaggaagtc
     4501 gagtttcgct tcgacagcga tggccaacgc ttgcggcggt tcaaatctaa accgcgtggc
     4561 gatgaagctc caccggtgct taccgcggac acgcgggccg cggggccgct gacgcgggac
     4621 caaccgttta cctatcttta tcccgagtgg gactttcgtg gcggcgctta tcgccagcgt
     4681 tggtgtcggg tgcgcgaacg cgttatggac caagggtcag cggatttcta tatggaagcg
     4741 ttggctgaac atcggccact ggtaggccag gtagtcaatc gcatcgagca tttgattccc
     4801 gagctgtttt gtaaggtgac ccgtcgcgac gacggcgagg atgtcgatct cgacgcggtg
     4861 atcgaacgat tcgtcgatcg gcgcacgggc ggaatgccta gcgagcagct ctattggcgg
     4921 cgcgagcgca cacagcgcga tgtggcggtg gctgtgctac tggacatgag cgcgactacc
     4981 gatgagtttg tccaactgga ggcggccaag gcgcaccggc cgacgggacc gagcgcccag
     5041 gcctacagcg attacttgaa gcgcctcgcc gccggcgtcg accaacgcgg taagccgctg
     5101 cggcggcgca ccatcgatat tgaaaaacag gcggccatcg tgctctgtca agccttggaa
     5161 aagatcggcg acagctacgc cgtctacgct ttttccggca gcgggcgcgc cgaggttgag
     5221 tttcatgtgg tcaaagactt ccacgagcgg ttgacgcagc ggatcggccg gcgcatcgat
     5281 cgcattcaac cggcgcatgg gacgcggatg ggcgcggcga tccgccatgc tctgggtaaa
     5341 ctggagcgag tagaagcgaa cacgcggcta ttgtttctct t
//
LOCUS       DASQKS010000582         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5777533, whole genome shotgun sequence.
ACCESSION   DASQKS010000582 DASQKS010000000
VERSION     DASQKS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5777533"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..781
                     /locus_tag="VE170_14860"
     CDS             83..781
                     /locus_tag="VE170_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16775.1"
                     /translation="MSLWPKVREELNSIVNQHFDTPEYHQLFTVKLTPARQKVWDAHY
                     PHYIKSRRDCWGAAAVKAPLDVKRAIWEHEKDELIFDKRMGSAHLTDQQMAEATAEAN
                     LLPGVRAALMAWMYLATTRPWIESLTVNHITERKNNPAIVKGGGFTSRFAHKRVADQG
                     GSIEKLDINTKVHMVADEDHSDMFEPIYDRHITDERIAQAVIRSALDSLAIDRAYRSA
                     LAVGMARIEESAAA"
     gene            901..2475
                     /gene="ggt"
                     /locus_tag="VE170_14865"
     CDS             901..2475
                     /gene="ggt"
                     /locus_tag="VE170_14865"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYJ16776.1"
                     /translation="MAQQFSVLAKNGMVASSQPLATLAGVEILMNGGNAIDAAIATAA
                     VLGVVEPSSIGIGSDAFCLFYSVKDRAIKALDASGRSPYAMGADFCRKSRFQEMPQRG
                     IHSVTVPGAVHGWTTLLNGYGTKKLSEVLQAAIRHADEGFPVAELTAEQWKESEARLK
                     ADEGGMINYLIGGRAPKAGEIFRNPRMAATLKRIAADGADYFYRGEVAAKLVRCSEKL
                     GGLFTPEDFADHRSDWVEPITANYRGYDVYEMPPTSQGFVALEMLKILEGFDLKAMGA
                     NSADGLHAMIEAKKLAFADRDRQLADRDFMKVDVRELFAAERTERLRGLIRMSNAAGE
                     YPNALGGTDTEYVAAADRDGNLVSFIQSNFMGFGSGVVEPETGIILQNRGHLFSLDAN
                     HPNVIGPHKRCAHTLMPGMIFTDGKPYLALGLKGGHVQPQVQVQLISNLIDHGMAPQA
                     AIGSARFNHIDGLKVGLEPEVPQAVKDELTARGHQVVGGNPESFGGAHMILIHPESGA
                     FVGGSDPRKGGCALGF"
     gene            2791..4386
                     /locus_tag="VE170_14870"
     CDS             2791..4386
                     /locus_tag="VE170_14870"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16777.1"
                     /translation="MGYFKDLREHIAALDKAGLLVKIDEQINKDTELMPLVRWQFRGL
                     PEEERRAFMFTNVIDAKGRKYDIPVVVGTLAATRQIYGFGLQCAPEEIVDRWNHAQSN
                     PIPPKIVDKAPVHEEVHIGASLLEHGALGEFPVPISTPGYDCAPFTTASHWFTKDPET
                     GLINVGNYRGHIKSPTRTGVFLSSSNHGGYHWRKCKERGIPLQAALVIGAPPCVAYTA
                     PSRIPFSVSELDVAGGLAGAAIEVVKCKTNDILVPANAEIVIEGEISTEFLEPEAPFG
                     ESTGYIAEKLLNPYFEVKCITHRKNPILATIISQMPPSESSVMRQVAAEGNYLRFLRD
                     QCNIPGIIEVAFHSIAVRQICVIKMKKINQAHVWQALHSMVGYNPATGKMVIAVDDDI
                     NPRNLDSLFWAMAFRMQPHRDVLIIRNRTPQMDPSVFPPGHEQGLSVDEIAGSSAMLM
                     DATRKWAYPPVSLPKKEYMERAKQLWERLELPKLKPQDPWHGYDLGLWSEDDEADAQA
                     AVKGDHYETGEKRAKLRVKVAKL"
     gene            complement(4553..>4773)
                     /locus_tag="VE170_14875"
     CDS             complement(4553..>4773)
                     /locus_tag="VE170_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16778.1"
                     /translation="IRKRFNMDEKLARESYDVVVNSFSRDGRVPLDGVDILLQIEKDA
                     KVIPATVTPQMIVDPSLAEEALKELGVK"
BASE COUNT         1043 a         1334 c         1441 g          955 t
ORIGIN      
        1 tagcatttcg cgcgcggtga gaaaacactc ttttgccgcg agcgaaggtg ttttggacgt
       61 tattatttag gaggatgggc aaatgagttt atggccgaaa gttcgtgaag agttgaacag
      121 catcgtcaac cagcatttcg atacgccgga gtatcaccaa ctgtttaccg tcaagctgac
      181 gccggcgcgg cagaaggttt gggatgcgca ctatccgcat tacatcaaaa gccggcgcga
      241 ctgttggggc gcggcggcgg tgaaggcgcc cctcgacgtc aagcgcgcca tctgggagca
      301 tgagaaggac gaactaatct tcgacaagcg catgggctcg gcccatttga ccgaccagca
      361 gatggccgag gccacggcgg aggcgaatct gcttcccggc gtgcgcgcgg cgctgatggc
      421 gtggatgtac ttggcgacga cccggccatg gatcgagtcg ttgacggtca accacatcac
      481 cgagcgcaaa aataatccgg ccatcgtcaa aggcggcggc ttcacgtcgc gcttcgcgca
      541 caagcgggtc gccgatcaag gcggcagtat cgaaaaactc gatatcaaca ccaaggttca
      601 catggtcgcc gacgaggatc actcggacat gttcgagccg atatacgatc gccacatcac
      661 cgacgagcgc atcgcccagg cggtgattcg gtcggcgtta gacagcctcg ccatcgaccg
      721 cgcctatcgc agcgcgctgg cggtcggcat ggcgcgcatc gaagaatcgg cggcggcgta
      781 gcgcgcgcag cgcgcttggt tcaaggttca agggttcaaa agttcaaggt cggaagcaga
      841 agtctcgcag ggtgggttaa ctgcggcgag cagcgtaacc cacctttctt ttgagaatcg
      901 atggcacaac aattttccgt gctagccaag aacggcatgg tggcttcgag tcagccgctg
      961 gcgaccctgg ctggagtcga gatcctcatg aacggcggca atgccatcga tgcggcgatc
     1021 gccaccgcgg cggtgttggg cgtggtcgag ccgtcgtcca tcggcatcgg cagcgacgcg
     1081 ttttgtctct tttattccgt taaggataga gcgatcaagg cgctcgatgc cagcgggcgg
     1141 tcgccctatg cgatgggtgc ggatttctgc cgcaaaagcc gcttccaaga gatgccgcag
     1201 cgggggatac attcggttac cgtgcccggg gcggtgcatg gctggacgac cctgctcaat
     1261 gggtacggca cgaagaaact gtccgaggtt ttgcaagctg cgatccgcca cgcggacgaa
     1321 ggctttccgg tggccgagct tactgcggaa cagtggaaag aatccgaggc gcggctgaaa
     1381 gccgacgagg gcgggatgat caactatttg atcggcgggc gcgcgcccaa ggccggcgag
     1441 attttccgca atccgcgcat ggcggcgacc ttgaaacgga ttgccgcaga tggcgcggat
     1501 tatttctatc gcggcgaggt cgccgccaag ctggtgcgct gctcggaaaa gctcggcgga
     1561 ttgtttacgc ctgaagactt tgccgatcat cgttccgatt gggtcgagcc aatcaccgcc
     1621 aattatcgcg gttacgatgt ttatgaaatg ccgccgacca gccagggatt cgttgcgctg
     1681 gagatgctca agatcctcga aggcttcgat ctcaaggcga tgggagcgaa ttccgccgac
     1741 ggccttcatg cgatgatcga ggcgaagaaa ttggcgttcg ccgaccgtga ccgccagctc
     1801 gccgatcgcg atttcatgaa agtcgatgtg cgtgaattat ttgccgccga gcgaaccgag
     1861 cggttgcgtg ggctgattcg aatgagcaat gctgccggcg aatatcccaa tgctttaggc
     1921 ggcacggaca ccgagtacgt cgcggcggcg gatcgcgacg gcaatctggt ttcgtttatt
     1981 caaagcaact tcatgggatt cggttccggc gtcgtcgagc ccgaaaccgg tattatctta
     2041 cagaatcgcg gccatctgtt ttccctcgac gctaaccatc ccaatgtcat cggcccgcat
     2101 aagcgctgcg cgcacacgct gatgcccggc atgatattca cagacggcaa gccttacctt
     2161 gcgttgggtt tgaaaggcgg ccatgtccag ccccaggtgc aggtgcaatt gatttcaaat
     2221 cttatagatc acggcatggc gccgcaggcg gcgatcggat cagcgcgctt taatcatatc
     2281 gatggattga aagtcggcct ggagccggaa gtgccgcagg cagtcaagga cgagctaacg
     2341 gctcgcggcc accaagtcgt cggcggcaat cccgagagct tcggcggcgc gcatatgatt
     2401 cttatccacc ccgagtccgg cgcctttgtc ggcggctccg atccgcgcaa gggcggctgc
     2461 gcgttgggat tttgaatcaa gaattttgcc gcaaagaacg caaaaagttt cggattcgtt
     2521 agccgcaaag aacgcaaagt tcgcaaaata atcccatcgg atcgactcaa atcttgttct
     2581 cgtccctcgc cctttgggag atggttaagg cagggcaaac gggcggtacg aaatccgccc
     2641 ataccacaat ctccgaagtc gaggaagtaa gagtcctgtc gatactttca ccgtgcaacc
     2701 ctcattgaga atccacgatc cgcttcactg gcaaactcat ctaaaatcgc atatcaccat
     2761 cgacaggaat tcactcaagg agattctgtc atggggtatt tcaaggattt acgggaacat
     2821 atcgcggcgc tggataaggc cgggctgctg gtcaagatcg acgagcagat caacaaagac
     2881 accgagttga tgccgctggt gcgctggcaa tttcgcggcc tgccggagga agagcggcgc
     2941 gctttcatgt ttaccaacgt gatcgacgcc aagggacgca agtacgacat tcccgtagtg
     3001 gtcgggaccc tggcggcgac ccggcaaatt tatggcttcg gtctacaatg cgcgccggag
     3061 gaaatcgtcg atcgctggaa ccatgcgcag tcaaatccga tcccgccgaa gattgtcgac
     3121 aaggcgccgg tgcatgagga agtgcatatc ggcgcttccc tgcttgagca tggcgccttg
     3181 ggtgagttcc cggtgccgat atccacgccg ggctacgatt gcgcgccttt taccacggcg
     3241 tcgcattggt tcaccaaaga tcccgagacc gggctgatca atgtcggcaa ctaccgcggc
     3301 catatcaaaa gcccgacgcg caccggcgtg tttctgagct cgagcaatca tggcgggtat
     3361 cattggcgca agtgcaaaga gcgaggtatt cccttgcaag ccgcgctggt catcggcgcg
     3421 ccgccttgcg tcgcttacac cgcgccgtcg cggattcctt tcagcgtgag cgagctcgac
     3481 gtcgccggcg gtttagccgg agcggccatc gaagtggtga aatgcaaaac caatgacatt
     3541 ctggttcccg ccaacgcgga gatcgtcatc gagggcgaaa tctccacgga gtttctcgaa
     3601 cctgaggcgc cgttcggcga gtccacaggg tacattgccg agaaactttt aaatccttac
     3661 ttcgaagtga aatgcatcac ccatcggaag aatccgattc tggcgacgat catcagccag
     3721 atgccgccga gcgagtcgag cgtcatgcgc caagtggcgg cggaaggcaa ttacctgcgc
     3781 ttcctgcgcg atcagtgcaa cattcccggc atcatcgagg tggcttttca cagcatcgcg
     3841 gtgcgccaga tctgcgtcat caagatgaaa aagatcaacc aggcccatgt ctggcaggcg
     3901 ctgcacagca tggtcggcta caatccggcc accggcaaga tggtcatcgc cgtcgacgac
     3961 gacatcaacc cgcgcaatct cgattcgctg ttttgggcga tggcgtttcg catgcagccg
     4021 caccgcgatg tcctgatcat tcgcaaccgc acgccgcaaa tggacccgtc ggtatttccg
     4081 ccgggacacg agcagggatt gtctgtcgat gaaatcgccg gctcgtcggc gatgctgatg
     4141 gacgcgaccc gcaagtgggc ctatccgccg gtgtcgttgc cgaagaagga atacatggag
     4201 cgcgccaaac agctttggga gcgcttggag ctgcccaaac tcaaacccca ggatccctgg
     4261 catggttacg atcttggctt gtggtccgag gatgacgagg ccgacgccca agcagcggta
     4321 aaaggcgatc attacgaaac cggcgagaag cgggcgaagc tgcgggtaaa ggtagcgaaa
     4381 ctgtgacggg aggccgaatt aattaaggtt caaatcgttc aatccggtca aaacgtttaa
     4441 atcgtgcata acgtagggtg ggtggaggcg tttagccctc cacccaccgt cttggtcggc
     4501 acatggcacc gaagagatgg gtatcgctac gctccaccca tcctacaaga acttatttca
     4561 ctcccaactc tttcaacgcc tcctccgcca gcgacgggtc gacgatcatt tgcggcgtga
     4621 cggtggcggg aatcactttg gcgtcttttt cgatttgcag gaggatgtcg acgccgtcga
     4681 ggggaacccg gccgtcgcgg ctgaaggaat taaccacgac gtcataggac tcgcgggcga
     4741 gtttttcgtc catgttgaaa cgtttgcgga tga
//
LOCUS       DASQKS010000583         6202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2447938, whole genome shotgun sequence.
ACCESSION   DASQKS010000583 DASQKS010000000
VERSION     DASQKS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447938"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VE170_14880"
     CDS             complement(<1..1043)
                     /locus_tag="VE170_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ16779.1"
                     /translation="MIETFTSLLHGFSIALTPLNLLWGFIGTILGTFIGVLPGVGPAL
                     TVAMLMPLTIKLDPTGALIMFAGIYYGAMYGGSTTTILLNTPGESASIATALEGNLMA
                     KNGRAGPALATCAIGSFVAGTISTILVTLLAPVVVEVALKFGPTEYFALMVFAFTTVS
                     AVIGSSTVRGLTSLFLGLLLGVVGIDSQTGKSRFAFGVPELLDGIDVVVLAVGLFAVG
                     EALYVGAYASRLEEKMEQIKGSLWMSKQDWKRSWPAWLRATAIGFPFGSIPAGGAEIP
                     TFLSYATEKRLTKHPEEFGHGAIEGVAGPEAANNASVTGALVPLLTLGIPTSATAAVL
                     LSAFQNYGISPGPL"
     gene            complement(1048..1548)
                     /locus_tag="VE170_14885"
     CDS             complement(1048..1548)
                     /locus_tag="VE170_14885"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ16780.1"
                     /translation="MKAKPAELSLSLGILALGVAAAVGTSQLPSAGGYARIGPNIAPA
                     VISGGLILLGLWLLYETFSGGWRNAIADDPAERGEHRFTPSAFIWVSAGLFAQILLIH
                     RAGFVLAQAALFCCVARGFGSSKWPRDLAVGFLLGLLVFFFFVKFLNVSLPAGWLKPL
                     LGGAGI"
     gene            complement(2054..3010)
                     /locus_tag="VE170_14890"
     CDS             complement(2054..3010)
                     /locus_tag="VE170_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16781.1"
                     /translation="MRKWFSSLLVALTVAATVVGAHAQPSLKMMIPANPGGGWDQTGR
                     NLANAMQSAKLVSSVQFENKGGAGGTIGLAQFINSAKGDPNAVMIGGMVMVGAILLEG
                     SPVNVSMVTPLARLTGEYEIIVVPASSPHKTMADLVKAFKANPGGISWGGGSAGGTDH
                     ILAGLIAKASGVDAAKVNYVPFKGGGEAIAAIVGGHVTAGVSGIGEFAEQIKGGRMRA
                     LAVSSPAKFDGYPSLKEQGIDVELANWRGVFGAPGITAAQTDALIKIVRGATESASWK
                     ETVKKLGWSPIFLSGEPYKKFIDEDTKRIAGIIDSLGIKTTK"
     gene            complement(3138..3575)
                     /locus_tag="VE170_14895"
     CDS             complement(3138..3575)
                     /locus_tag="VE170_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HYJ16782.1"
                     /translation="MIAVDTNLLVYAHREDSPWHEAAYKIVEQLSESRAPWAIPWPCL
                     HEFIAIVTHPRIYNPPTPLAAALDQVDSWIESPSLSLIRESEGYWPSLKNVLEQGRIA
                     GPKVHDARIAAICLLHGVRELWTADRDFSRFPTLTTRNPLVSL"
     gene            complement(3572..3817)
                     /locus_tag="VE170_14900"
     CDS             complement(3572..3817)
                     /locus_tag="VE170_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYJ16783.1"
                     /translation="MKTTVEISNNLLEEAKRLAAREGTTVKALIEQGLRQIIAAKKKP
                     GVFHLRKATFKGQGLQPGASTATWEQIRDMAYEGHGG"
     gene            complement(3831..5540)
                     /locus_tag="VE170_14905"
     CDS             complement(3831..5540)
                     /locus_tag="VE170_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004504099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ16784.1"
                     /translation="MRVLLIQPPVQDFYDTDVRLQPIGLCYLKAAVNTHLPGIDIIVK
                     DYHSGFGRKTVAIPKELRYLTDYYGVADKSPFSTFHRYYHFGKSFDDIEKEIARLKPD
                     IAGISSLFTPYFRDALAVAARVKKASNAIVVMGGSHASAVPESLLASAHVDYVIRGEG
                     ERPFVEFLRCLRDHKPVETVPNVAYKRHGEFILNPIEENFPLEDLPMPDLSDCAPASY
                     TLAGKPLTFMITSRSCPHKCSFCSVHTTFGTDYRRRSLEQVLEEIDLRYRQGYRVIDF
                     EDDNLTYYKQTFKELCRRLIARFPAREMQFVAMNGISYLSLDEELLELMYAAGFSHLN
                     LALVSSDKTVRETTKRPHTLEAYLRVVHKAAALGFKIVSYQILGLPNESVDSMIQTLA
                     FNARLPVLLGASPFYQTPNSPIARGLELNESDFIKARLTALAIETKGFTRADIYTLFV
                     TTRIVNFLKGLSIGGSTSLPELMTRTWPEPRTQVGFDLLKQMSETGRLCFATGQGLQE
                     NKKFCNALCERVLHEIGTIRCQNGATISLEKISQKPPAPSLPQADCRDKLQQNNLTLS
                     HIK"
     gene            5652..>6202
                     /locus_tag="VE170_14910"
     CDS             5652..>6202
                     /locus_tag="VE170_14910"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16785.1"
                     /translation="MKIEKITTRKLEMPYTKPLVTATNNFTVARGLLVTVETNGGVEG
                     YGYADLFPRAGETPETAQHVIDTVLKAKTIGRELEDLGSIRADIDHALTGHPRAKSAL
                     EGALYDALARSYHMPLSVMLGGKCRSEIKVIKMVSVGEPEAMAAEAKELVAEGLALKL
                     KVSGRIDQDLPRVAAVRQAVGDHV"
BASE COUNT         1321 a         1728 c         1857 g         1296 t
ORIGIN      
        1 agcggccccg gtgagatgcc gtagttctga aaggcgctga gcaagaccgc ggcggtggcc
       61 gaggtcggaa tgccgagggt taacagcggc accagcgcgc cggtgaccga ggcattgttg
      121 gccgcttcgg ggccggcaac gccttcgatg gcgccgtggc cgaattcctc cgggtgcttc
      181 gttaatcgct tttccgtcgc ataggatagg aaggtgggga tctcggcacc gcccgctgga
      241 atcgagccga agggaaaacc gatggcggtg gcgcgcagcc aggcgggcca agaacgtttc
      301 cagtcttgct tcgacatcca tagcgaacct ttgatctgtt ccatcttctc ttcgaggcgg
      361 ctcgcgtagg ctcccacata gagcgcctcg ccgacggcga acaggccgac ggcgagcacg
      421 accacgtcga tgccgtccaa cagttccggc acgccgaacg cgaagcgcga cttgccggtt
      481 tggctgtcga tgccgacgac gccgagcagt aagccgagaa acaaactggt taaacctctt
      541 acagtggaac tgccgatgac tgcggacacg gtggtaaagg caaacaccat cagggcgaaa
      601 tattccgtcg ggccgaactt gagcgcgacc tcgacgacga ctggcgcaag cagggtgacg
      661 agaatcgttg agatggtgcc ggcgacgaag gagccgatgg cgcaggtcgc cagcgccggg
      721 ccggcgcggc catttttcgc cattaaattt ccttccagcg cggtggcgat ggatgccgat
      781 tcgcctggcg tattcaacaa gatcgtcgtc gtcgagccgc cgtacatcgc gccgtagtag
      841 attcccgcga acatgatcag cgcgccggtg gggtcgagct tgatggtcag cggcatcaac
      901 atggcgacgg tgagcgccgg gccgacgccg ggcagcacgc cgatgaaggt gccgagtatg
      961 gtgccgatga aaccccaaag cagattcagc ggcgtcaggg cgatactgaa gccgtgcaac
     1021 aaggaggtga aagtttctat catcgcacta gatgccggcg ccgccgagca agggcttcaa
     1081 ccatcccgcc ggtagactca cgtttaagaa ttttacaaag aagaagaaaa ccaaaagacc
     1141 gagtagaaaa cccacggcca ggtcgcgcgg ccatttgctg ctgccaaaac cgcgggcaac
     1201 gcaacagaac aatgcggctt gcgcgagcac gaaaccggcg cgatggatca aaaggatttg
     1261 cgcgaataga ccggcgctca cccagataaa tgcgctcggg gtaaagcgat gctcgccgcg
     1321 ttctgccggg tcgtcggcga tggcgttgcg ccagccgccg ctaaaagttt cgtaaaggag
     1381 ccataggccc agaagaatga ggccgccgga aataaccgca ggggcgatgt tggggccgat
     1441 gcgcgcgtaa ccgccggcgc tgggcagctg cgatgttcct acggcagcgg cgactcccaa
     1501 agccaagatg cccagggaaa gactcagctc ggcgggtttg gctttcatga gttggtagaa
     1561 aatggtccgc tgtggtaata gctacgccga tcaagacttc atcattcacc gcgacgtgtc
     1621 cgtgagcttg cgctctccta gagagcatga aaatggttgg cattcccgga acatgctccc
     1681 ctcctcgatt cgtcattccc gcgcatgcga gaatccaggt ttgttctgcg ccaaattagc
     1741 ctggataccc gctttcgctg gtatgacggc gccccggagg ctcttcgcta tgcaatcatc
     1801 cctcatatgc atttttcgag ggaggcacga aggtctcgga taattaatat tccgaacttc
     1861 gtgctcttct atgcttaata atttgtgcgg cttgggcaaa tcgtccgagc gctaaatccc
     1921 agagagcggg tttcaaaccc gcccctacca atcccgatcc tttttgcgcc ctcttgcctt
     1981 ctttgcggcc attcttcaat tcggttgcgg ctgtgccgcg ttaggtcctt cgttgaaaga
     2041 aatttctacc tgcctatttc gtggtcttta taccgagcga atcgatgatc ccagcgatgc
     2101 gcttggtgtc ttcatcgatg aacttcttgt agggttcgcc gctcaaaaaa atcggcgacc
     2161 agccgagttt cttcacggtc tctttccatg acgccgattc ggtggcgccg cgcacgatct
     2221 tgatcaaggc gtcggtctgc gccgcagtga tgcccggggc gccgaaaacg ccgcgccaat
     2281 tggcgagttc aacgtcgatg ccctgttctt tcaatgacgg atagccgtcg aacttcgccg
     2341 ggctggaaac cgccagcgcg cgcatgcggc cgcctttgat ctgctcggcg aactcaccga
     2401 ttccggaaac accggcggta acgtggccgc cgacgatggc ggcgatcgct tcgccgccgc
     2461 ccttgaaagg cacgtaattc accttcgcgg cgtcaacgcc gctcgccttg gcaatcagtc
     2521 cggccaggat gtgatcggtg ccgccggcgg agccgccgcc ccaggaaatt ccgccgggat
     2581 tggctttaaa agctttgact agatccgcca tcgtcttgtg cggcgagctg gccggcacga
     2641 cgatgatttc gtattcgccg gtcaagcgcg ccaacggcgt gaccatgctc acatttaccg
     2701 gcgacccttc gagtagaatg gcgccgacca tgaccatacc gccgatcatg accgcgttcg
     2761 ggtcgccctt ggccgagttg atgaattgcg ccaggccaat cgtgcccccg gcgccgccct
     2821 tgttctcgaa ttgcaccgac gaaacaagtt tggcgctttg catcgcattc gccaaattgc
     2881 gcccggtctg atcccaaccg ccgccggggt tggccgggat catcattttg agactaggct
     2941 gggcatgagc tccgacgacc gtcgccgcca cagtcagagc cacgagcagg ctggaaaacc
     3001 atttacgcat aatgcctcct tgcgattaac cggttctcaa ttcgaaccaa gttgacgatg
     3061 tatagtagac agcgaataaa ttgacaagct tcaactggct aactcgggct gggaactggc
     3121 gggcaaaaca tttctgatca caaggataca agtggattcc gggtagtcag cgtcggaaaa
     3181 cggctgaagt cgcgatccgc tgtccacagt tctcgcaccc catggagcag acagatcgct
     3241 gcgatgcggg catcgtgaac tttagggccg gcaatacggc cttgttcaag gacatttttt
     3301 aggctcggcc aataaccttc ggattcgcga atcaagctta gactcggaga ctcgatccaa
     3361 gaatcgactt gatccaaagc ggcggcaagg ggtgttggcg ggttataaat acgcggatga
     3421 gtaactattg cgatgaattc atgcaggcaa ggccatggaa tggcccatgg cgcacggctc
     3481 tcggatagtt gttcgacgat tttgtaagct gcctcgtgcc agggtgaatc ttcgcgatgg
     3541 gcgtagacta ggagattcgt gtcgactgcg atcatccgcc gtggccttca taagccatgt
     3601 cacggatctg ctcccacgtc gctgtcgaag ctcctggttg aagtccctgg cctttgaacg
     3661 tggctttcct taagtggaag acgccgggct tctttttcgc cgcaattatc tgtcgcaggc
     3721 cctgttcgat cagggccttg acggtcgtcc cttcccgcgc cgccagcctc tttgcttctt
     3781 ccaacagatt attggatatc tctacggttg tcttcatatg ggtatatgta tcatttgata
     3841 tggctgagcg tcaaattgtt ttgctgtaac ttatcccggc agtctgcctg cggcagagat
     3901 ggcgctggcg gtttctgact aatcttttca agcgaaatcg tcgcgccgtt ctgacatcgg
     3961 atcgtgccga tctcgtgtaa gacgcgttcg caaagcgcat tacaaaactt cttattctcc
     4021 tgcaaacctt ggccggttgc gaaacatagg cgaccggttt cggacatctg tttcagcaag
     4081 tcgaagccga cctgggttcg cggttcaggc caagttcggg tcataagttc cggcagactt
     4141 gttgacccgc caatggacag ccctttgaga aagttcacaa tccgggtggt tacaaacagc
     4201 gtgtaaatat cggcgcgcgt gaaacctttc gtctcgatcg ccagagcggt gaggcgggct
     4261 ttgatgaaat cgctttcatt gagttccagg ccgcgggcga taggcgagtt gggcgtctga
     4321 taaaaaggcg aggcgccgag cagcaccggc aggcgcgcgt tgaacgccaa ggtctggatc
     4381 atgctgtcca cggattcgtt cggcaggcca aggatttggt atgacacgat tttgaagccc
     4441 agggcggcgg ccttgtgcac gacacggaga taggcctcga gggtgtgcgg ccgcttggta
     4501 gtctcgcgga cggttttgtc ggaactgacg agcgctaggt tcaagtgcga gaagcccgcg
     4561 gcatacatca gctccaatag ttcttcgtcc aagctcagat agctaatgcc attcatggcg
     4621 acgaactgca tctcgcgtgc cgggaagcgc gcgatcagac ggcggcacag ctctttgaac
     4681 gtctgcttgt agtaagtcag attgtcgtcc tcgaagtcga ttacccggta gccctgtcgg
     4741 tagcgcaagt cgatttcttc cagcacctgt tcgagcgagc gtcggcgata gtcggtgccg
     4801 aaggtcgtgt gcaccgaaca aaacgagcat ttgtgcgggc agctgcgcga cgtgatcatg
     4861 aaggtcagcg gtttgccggc aagtgtgtag ctcgccggtg cgcagtcgct cagatccggc
     4921 atcggcagat cctcgagggg aaagttttct tcgattggat tgaggataaa ctcaccatgc
     4981 cgtttgtagg ccacgttggg gacagtctcg acgggcttgt gatcccttag acaacgaaga
     5041 aattccacaa acggccgctc gccttcgccg cggatgacgt aatcgacatg ggccgacgcc
     5101 agcagcgatt ccggcaccgc cgaagcgtga gacccaccca tgacgacgat ggcattcgaa
     5161 gccttcttga cccgcgcggc cactgccagc gcgtcgcgaa aatacggcgt gaacagcgag
     5221 gagatgcccg ctatatccgg cttcaggcgg gcgatctctt tctcgatgtc gtcgaaggat
     5281 ttgccgaagt ggtagtagcg atgaaaggtc gagaacggcg atttatccgc gacgccataa
     5341 tagtcggtca ggtaacgcag ttccttcgga atcgccacgg tcttgcggcc gaagccgctg
     5401 tgatagtcct taacgataat atcaatgccc gggagatgtg tgttgaccgc ggctttgaga
     5461 tagcaaagac cgatgggctg caggcgcacg tcggtgtcgt agaaatcctg aaccggcggt
     5521 tggatgagca aaactcgcat ttcacaatga ggtatatcct atgggattac cgcgacggaa
     5581 gcggttgtat tgtcggacca acaatattaa tgatctgtgg aaatatcgca ttgcacgccc
     5641 aggagcggcc gatgaagatc gaaaaaatca ccacgcgcaa gctcgagatg ccttacacca
     5701 agccattggt aacggcgacc aacaacttca cggtcgcgcg cggtttgctg gtgacggtgg
     5761 agaccaacgg cggcgtcgaa ggctacggtt atgccgatct gtttccgcgc gcgggggaga
     5821 cgccggagac ggcgcagcat gtcatcgaca ccgtgttgaa agccaaaacc atcggcaggg
     5881 agttggaaga tctgggcagc attcgcgccg atatcgacca tgctttgacc ggccacccgc
     5941 gggcgaagtc ggctttggag ggcgcgctgt acgatgccct ggcgcgcagc tatcatatgc
     6001 cgctgtcggt gatgctcggc ggcaagtgcc gcagcgaaat caaagttatt aaaatggtca
     6061 gcgtcggcga acccgaggcg atggcggcgg aagccaaaga gctggtcgcc gaaggattag
     6121 cgttgaagct taaggtcagc ggccggattg accaagatct gccgcgggtc gccgccgtgc
     6181 gccaagcggt cggcgaccat gt
//
LOCUS       DASQKS010000584         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4498464, whole genome shotgun sequence.
ACCESSION   DASQKS010000584 DASQKS010000000
VERSION     DASQKS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4498464"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..723)
                     /locus_tag="VE170_14915"
     CDS             complement(4..723)
                     /locus_tag="VE170_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ16786.1"
                     /translation="MRYELYYQPSIQGRGEFVRLALEDAGADYVDVARDPAFGRPGIM
                     KILEDPSVEHPSYAPPFLKAGRLMIGQTANILQYLGPRLGLVPKDQASRLWVHQLQLT
                     VTDWVAEVGNTHHPIANVLYYEEQKGEAKKRASFFTSQRIPKFFGYFEKILAKNSKGS
                     VYLLGKQICYADLSLFQMVDGLRYAFPRTMAKIEPTIPRAIAVHDRVAARPRLKKYLA
                     SPRRLAFNTNGIFRYYPELDE"
     gene            complement(829..1866)
                     /locus_tag="VE170_14920"
     CDS             complement(829..1866)
                     /locus_tag="VE170_14920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16787.1"
                     /translation="MKQSRLDNPIISYQTRRLVIAVGLVAGCLVSVSGAQDKKPAAAT
                     IGYSAISGSFAPLWVAQDLGLFSKHGLDAKLAYIQGNRVMLSALTAGEIQFYQGGAEG
                     LIRLVSGGGDGVLIATQYNTVAHYVLLTDPSIVKLEDLRGKRIALDPTSPTYGYFLKV
                     LEQVGLKKEDVQFVQFGSAGQPERTAAVLRKQAAATILTAPNTYAAEKQGLRKFSIIR
                     ELGIRQLITITGTTRKYLRERRDGAEAFMRGYVEGLAHVRNQRDSTIRVIAKYTRQRD
                     TEILNKFYDEMASDLPRIPYIDEISARATIDAMQTQGPPLPKVDVKGIFDNSILRAFE
                     TSGLIDKLAPR"
     gene            complement(1996..>2327)
                     /locus_tag="VE170_14925"
     CDS             complement(1996..>2327)
                     /locus_tag="VE170_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16788.1"
                     /translation="KALTSIKEGMGGSNGFGTVAVMANAPHPNAAKVYINWLLGKEGQ
                     DLYGRALTQGTRRFDVDTKWLSRYNTPAAKDVSTPEEFEKVRFFGEDVVANWREPAGE
                     FARKILK"
BASE COUNT          434 a          640 c          717 g          536 t
ORIGIN      
        1 tctctactca tcgagctccg gataataacg aaagatgccg ttggtgttga acgcgagacg
       61 gcgcggcgaa gccagatatt tcttgagccg cggtcgggcg gcgacccggt cgtgcacggc
      121 gatggcgcgc gggatggtcg gttcgatctt cgccatggtg cgtggaaagg cgtagcgcag
      181 cccgtcgacc atctgaaaca gcgacagatc ggcatagcaa atttgtttgc cgagcagata
      241 gacgctacct ttgctgttct tggcgagaat tttttcgaag tagccgaaaa atttcggaat
      301 gcgttgggaa gtaaagaatg aggcacgttt tttcgcttcg cctttttgtt cttcgtagta
      361 aagcacgttg gcgattggat gatgggtgtt gccgacctcg gcgacccagt cggtgaccgt
      421 gagttgcaat tgatgcaccc acagccggct cgcttggtct ttcggcacca gccctagccg
      481 tgggccaagg tactgaagaa tattcgcggt ctggccgatc atgagcctgc cggcttttaa
      541 gaagggcggc gcataggatg gatgctcgac gctgggatct tcgagaatct tcatgatgcc
      601 gggacggccg aaggcgggat cgcgagcgac gtcgacgtag tcggctccgg cgtcttccag
      661 cgccaatcgg acgaattcac cgcggccttg aatggacggt tggtagtaga gttcgtagcg
      721 catattcgtg atcccttttt ttgccaatgc cagcagtctg ccagcgagtt agcgagcatg
      781 taaaatagag gatggccatt ttcgctcctc catcttagat ctcttttcct agcgcggtgc
      841 cagcttatcg atcaagccgc tggtctcaaa cgccctaagg atgctgttat cgaatatccc
      901 cttgacgtcg actttcggca gcggcgggcc ctgagtctgc atggcgtcaa tcgtcgcccg
      961 cgccgaaatt tcgtcgatgt agggaatccg cggcaggtcc gaagccatct cgtcgtaaaa
     1021 tttattcaag atctcggtgt cgcgttgccg cgtgtacttg gcgatcaccc ggattgtcga
     1081 gtctctttgg tttcggacat gggcgagtcc ttcgacatag ccgcgcatga acgcctcggc
     1141 gccgtcgcgc cgctcgcgca ggtattttcg cgtcgtgccg gtgatcgtta tcagctggcg
     1201 gatgccgagc tcgcggatga tcgaaaactt tctcagcccc tgcttctcgg cggcgtaggt
     1261 attcggcgcc gtcaggatgg tggcggcggc ttgcttgcgc agcaccgcgg cggtgcgttc
     1321 cggttggccg gcgctgccga attggacgaa ctggacatcc tctttcttga ggccaacctg
     1381 ctccaggact ttcaaaaaat aaccgtaggt cgggctggtg ggatcgagag cgatgcgctt
     1441 gccgcgcaga tcttccagct tgacgatgct cggatcggtg agcaagacat agtgggccac
     1501 ggtgttgtac tgggtcgcga tcaatacgcc gtcgccgccg ccggaaacca gccggatcaa
     1561 tccttcggcg ccgccctggt aaaattggat ctcgccggcg gtgagcgccg acaacatgac
     1621 gcggttgccc tggatgtagg cgagcttggc gtccagtcca tgtttgctaa acagacccag
     1681 atcttgcgcc acccacagcg gtgcgaaact gccgctgatc gcggagtaac cgatggtggc
     1741 ggcggcgggc ttcttatctt gcgcgccgct caccgataca aggcaacccg cgaccagccc
     1801 gaccgcgatc accagccggc gcgtttggta tgaaataatc ggattgtcaa ggcgcgattg
     1861 cttcatgaag gcacgaaggg cacgaaggtt tcgttgtcgc gacgacaaat ggttcgccac
     1921 gaattcggct ttgtcgggga tagtgagcgc agccgaggca tcgtcgtcgt gatgcagagt
     1981 attttcatag gttcgctact tcaatatttt ccgcgcgaac tcgccggccg gttcgcgcca
     2041 gttggcgacc acgtcttcgc cgaagaagcg tactttctcg aactcctccg gcgtggagac
     2101 atctttggcc gccggggtgt tgtaacggct taaccatttg gtgtcgacgt cgaagcggcg
     2161 ggtgccttgg gtgagggcgc ggccgtagag atcctggcct tccttgccaa gcagccaatt
     2221 gatgtagact ttcgccgcgt tcggatgggg cgcattggcc atgaccgcga ccgtaccgaa
     2281 gccgttgctg ccacccatgc cttctttgat cgatgtcagc gctttga
//
LOCUS       DASQKS010000585         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_753227, whole genome shotgun sequence.
ACCESSION   DASQKS010000585 DASQKS010000000
VERSION     DASQKS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753227"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2366)
                     /gene="clpA"
                     /locus_tag="VE170_14930"
     CDS             complement(78..2366)
                     /gene="clpA"
                     /locus_tag="VE170_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HYJ16789.1"
                     /translation="MISKDLEATLNGALKEAKTRRHEYVSLEHLLFALLRDKDGKSTI
                     LACGGDLEKLHKSLEEFFDGQMETLPAGVDREPQQTLSFHRVLQRALIHAQSAERKEV
                     NGSNVLIAMFREPDSYAVYLLEEQGITRFDLVNYVSHGVSKIGGDEEPSPQADDEDHD
                     HEDGERPARRVNPLEAFTVNLVEKAGQGHIDPLIGRADEVERTIHVLCRRRKNNPIYV
                     GDPGVGKTAIAEGLALKIHHGEVPDALKSAVIYALDMGALLAGTKFRGDFEARLKGVL
                     NGLKKHPDSILFIDEIHTIVGAGATSGGSMDASNILKPALASGELKCIGSTTYHDYKS
                     YFERDRALARRFQKIEVPEPSHDEAIKILEGLKPHYEKHHGVHYSAGAIRAAVKLSAK
                     HINDRCLPDKAIDVIDEIGAAVKIQPADKRRKSIGPKDVEKMVAKIAKIPPRSVSSSD
                     KEQLQTLERDLKLVVFGQDPAIDMLASTIKLSRSGLGHPEKPIGCFLFAGPTGVGKTE
                     LAKQLAKTMGIEFLRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGLLTDAINRNPYAVL
                     LLDEIEKAHPDLFNILLQVMDHATLTDNNGKKADFRNIVLIMTTNAGAREMSGTPIGF
                     GDRTNVGKGKEAIEKMFTPEFRNRLDAMITFNALNQENIERVVDKFIIELDQQLNERK
                     VFLELTNRARQWFAERGYDPTFGARPMARLIQNEIKRVLADEILFGKLQNGGKVQVDE
                     QDGKLVFSYTTTVESAPVPTPA"
     gene            complement(2369..2677)
                     /gene="clpS"
                     /locus_tag="VE170_14935"
     CDS             complement(2369..2677)
                     /gene="clpS"
                     /locus_tag="VE170_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HYJ16790.1"
                     /translation="MSRREPESEVVTETKKKVKRPPLYKVLLHNDDYTTKEFVVQVLQ
                     YVFHKEQAEALQIMLHVHKKGIGVAGVYTYEIAETKVTLVENLARQHEYPLKCTLEEA
                     "
     gene            2861..3721
                     /locus_tag="VE170_14940"
     CDS             2861..3721
                     /locus_tag="VE170_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ16791.1"
                     /translation="MLSNKVSEIMTTDLVKATVSATVFSVMETMVAEDVGRIIITDDE
                     VPVGIFTEKDVLKRVMGTAIDAKKASIKDVMTAPVRAVREETHIVDAFARMYRGKYRH
                     LLVRGRRGKIIGIVSMRRILNIAVELGQGISESRTVGDIPAAPITTVDESSTVHATVN
                     LMNQRGLTAVIVTAAGTPTGIFTERDVLRRVATVNMDRQQTPVRQVMTAPLIAVPRAT
                     LVGDVLAEMYRRDIRNMPVEEAPNQLLGLIAMPEILQYAQAFNIDEQVRRSWQEVAQA
                     LDTDDQYTPG"
     gene            3799..>4908
                     /locus_tag="VE170_14945"
     CDS             3799..>4908
                     /locus_tag="VE170_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HYJ16792.1"
                     /translation="METPLINDLNICVATENGSGSASSNNILFKAIFKMGIPCSSKNM
                     FPSNIQGLPTWYQIRANGDGYMSRKDVIDVMVMFNDTTAAKDIYRVRDGGVILYDDST
                     ALSPTLKRDGVQYFGFPANKLVAKLVPASPLRAKQRNMVYVGALAYLFGIDMSVIKAV
                     LEDTFGKKPAVIESNLVCIDAGYQYCKEQGYKQNIAHLEAIPDGNKGKIITEGNTAAA
                     LGSIYGGASVICWYPITPSSSLAEAMEYYLPRLRKQIDGKKPYAIVQAEDEIASAGMI
                     VGAGWAGARSMTSTSGPGVSLMNESIGLAYYAEIPCVFFIIQRGGPSTGLPTRTQQAD
                     ISLMYGASHGDTRHIILIPHDMNSCFDLARQSFNYA"
BASE COUNT         1065 a         1355 c         1326 g         1162 t
ORIGIN      
        1 ccgtgagaat aatctggtcc atgaaaaagc cggcgcgcga gtaagcgaaa tcgcgcggtc
       61 agaaatcgcc gaccggtcta cgccggggtt ggcaccggcg ccgattccac cgtcgtcgta
      121 taagaaaaga ctagcttgcc gtcttgctcg tcgacttgga ccttaccgcc gttttgcaat
      181 ttgccgaaaa gtatctcgtc ggccagaacg cgcttgattt cgttttgaat cagccgcgcc
      241 attggccggg cgccaaaagt cggatcgtag ccgcgctcgg cgaaccactg gcgtgcacgg
      301 ttggtgagtt cgaggaagac cttgcgttca ttcaactgct gatccagttc gatgatgaac
      361 ttatcaacca cccgctcgat attttcctgg ttcagcgcgt tgaaggtgat catggcgtcg
      421 agccggttgc gaaactcagg ggtaaacatc ttctcgatcg cttctttacc cttgccgacg
      481 ttggtgcggt cgccgaatcc gatcggcgtg ccgctcattt cgcgtgcccc ggcattggta
      541 gtcatgatca gcacgatatt gcggaagtcg gccttcttgc cgttgttgtc ggtcaaggtc
      601 gcgtggtcca tcacttgcag caagatattg aatagatcgg gatgagcctt ttcgatctca
      661 tcgagtaata gcaccgcata gggattgcgg ttgatcgcat cagtcagcaa cccgccctgg
      721 tcgaagccga cgtagcccgg cggtgcgccg atcaaacgcg acacggtgtg cttttccatg
      781 tattcgctca tgtcaaagcg gagaaactcg atgcccatcg ttttagccag ctgtttggcc
      841 agctcggtct tgccaactcc agtcggaccg gcgaagagaa aacagccgat cggtttttcc
      901 gggtgaccca gtcctgaccg ggaaagcttg atcgtgctcg ccaacatgtc gattgcggga
      961 tcttgaccga agaccaccag cttgagatca cgttccagag tctgcaactg ttccttgtcc
     1021 gacgacgaca cgctgcgcgg cgggatcttg gcgatcttcg caaccatctt ttcgacgtct
     1081 ttggggccga tgctcttgcg ccgtttatcc gcaggttgaa tcttgaccgc ggcgcctatc
     1141 tcgtcgatca cgtcaatcgc tttatcaggc aggcaacggt cattgatatg cttggcggaa
     1201 agctttaccg cagcgcggat cgccccggcg ctgtagtgca cgccatgatg tttctcgtag
     1261 tgcggtttta gcccttcaag aatcttgatc gcttcgtcat ggctcggttc gggcacctcg
     1321 atcttttgaa accggcgggc caaggcgcga tcccgttcga aatagctttt gtagtcgtga
     1381 taagtcgtcg agccgatgca tttcaattca cccgaagcta aagcaggttt gaggatattg
     1441 gaagcatcca tcgaaccgcc actggtcgct ccggcaccga cgatggtgtg gatctcgtcg
     1501 atgaaaagaa tcgagtcggg atgcttcttg agtccattga gcacgccttt aaggcgcgcc
     1561 tcaaagtcgc cgcgaaactt ggttccggcc aacaaagcgc ccatgtctag ggcataaatc
     1621 accgcgctct tgagcgcgtc gggaacttca ccatgatgga ttttcagcgc caacccttcg
     1681 gcgatggctg tttttccgac gccggggtcg ccaacataga ttggattgtt cttgcgccgc
     1741 cggcatagca catgaatggt tctttcaacc tcgtcggcgc ggccgatcaa cggatcgata
     1801 tgaccctggc ccgctttctc aacgagattg acggtaaatg cctccagcgg attgacacga
     1861 cgcgccggac gctcgccgtc ctcgtgatca tgatcctcgt catcggcctg gggtgacggc
     1921 tcctcgtcgc cgccgatctt cgacacacca tgagaaacgt aattgaccag atcgaagcgc
     1981 gttatccctt gctcttccaa aaggtagacc gcgtaggagt cgggctcgcg aaacatggcg
     2041 atcaggacat tgctaccatt gacttcctta cgctccgccg attgcgcatg aatgagcgcg
     2101 cgttgcagca cccggtgaaa gcttaaagtc tgttgcggct cgcggtcgac tcctgcaggc
     2161 aacgtttcca tctgcccgtc gaaaaactcc tccagtgact tgtgaagctt ttcaagatcg
     2221 ccgccgcaag cgaggatcgt gcttttaccg tctttgtcgc gcagcagagc gaacagcaga
     2281 tgctccaggc tgacatattc atgccggcgg gttttggctt ctttgagagc tccgttaagt
     2341 gtcgcttcga gatctttgct aatcatggtt aggcctcttc caacgtgcat ttcagtggat
     2401 attcatgctg gcgcgccagg ttttcgacca gcgtgacttt agtttccgcg atttcgtaag
     2461 tatagacgcc ggcaacgccg atgccttttt tgtggacatg gagcatgatc tgcagcgctt
     2521 cagcctgctc tttgtgaaag acatactgca agacctgcac gacgaactcc ttagtagtgt
     2581 aatcgtcgtt atgcagcaat actttataaa gcggcggccg tttgaccttt ttcttggtct
     2641 cggtgactac ctcggattcc ggttctctgc ggctcatgga ttccttggcc tccctgaaaa
     2701 cacaggaaat ctaacatgct gccaaggcag cccgcaatgc tcgccggctc cttgacaata
     2761 aattgcttgt agcccccgat tcccagagtg atatagttca ttgggcaatt gtcgagcctt
     2821 cgagggcgct taggctcgtc attatctcag gaggtgctct atgctatcta acaaagtcag
     2881 cgagatcatg accactgact tggtcaaagc aacggtatcc gcgactgttt tcagtgtaat
     2941 ggaaaccatg gtcgccgagg acgttgggcg tatcattatt accgatgacg aggtgccagt
     3001 aggcattttc accgagaagg acgtgctcaa gcgcgtcatg ggcacggcga ttgatgctaa
     3061 aaaagccagc ataaaagatg tcatgactgc acccgttcgg gctgtgcgcg aagaaacaca
     3121 cattgtcgac gctttcgcca ggatgtatcg cggcaaatac cgtcacctgc tggtacgggg
     3181 ccggcgcggt aaaatcatcg gcatcgtttc catgcgccga attcttaata tcgccgtgga
     3241 attgggtcag gggatcagcg aatcgcgaac cgtaggcgat atccctgccg cgccgatcac
     3301 caccgtcgat gaatcctcga cggtccatgc aaccgtgaat ttgatgaatc aacggggcct
     3361 caccgcggtc attgtcaccg cggcgggaac cccgacggga atcttcaccg agcgcgacgt
     3421 actgagacgg gtcgcgacag tgaacatgga tcggcagcaa acaccggtaa ggcaagtcat
     3481 gaccgcgccg ttgattgcgg tgccgcgcgc tactctagtg ggcgatgtgc tggccgaaat
     3541 gtaccgccgc gacattcgca acatgccggt ggaagaagcg ccaaaccaac tcctcggtct
     3601 gatcgccatg ccggagattc tccaatacgc acaagcgttt aacattgatg agcaggtgcg
     3661 ccggagttgg caggaagtcg cccaggccct cgataccgat gatcaatata cccccggcta
     3721 gagggcgtaa atcgttgtct tttaaagcga ttatgttaga attatagtgt tgtcagaaag
     3781 ttcgaaggag gctgtatttt ggagacaccg ctaatcaatg atttaaacat ctgcgtcgcc
     3841 actgaaaatg gttccggtag cgcatcgtcg aacaacattc tgttcaaggc gatattcaag
     3901 atgggcatac cctgttcatc gaagaacatg tttccgtcga acattcaggg gttgcccacc
     3961 tggtaccaga ttcgcgccaa tggcgacggc tatatgagcc gtaaggatgt catcgacgtg
     4021 atggtgatgt tcaacgacac cacggcagcg aaagacattt accgcgtgcg cgatggcggg
     4081 gtgattttat acgatgactc cacagcgctt tcgccgactt taaagcgcga cggtgttcag
     4141 tatttcggct ttccagcaaa taaactcgtg gccaagctgg ttccggcgtc gccattgcgc
     4201 gccaaacagc gcaacatggt ttatgtcggc gcgttggcct atttattcgg catcgatatg
     4261 tcggtcatca aagcggttct cgaggatact ttcggcaaga aaccggcggt tatcgagtcg
     4321 aacctggtct gcatcgatgc cggctatcaa tactgcaaag aacaaggcta caaacagaac
     4381 attgctcatc tcgaggcgat ccccgacggc aacaaaggca agatcatcac cgaaggcaac
     4441 accgcagccg ctctcggctc gatttacggc ggcgcctcgg taatttgttg gtatccaatc
     4501 acgccttcaa gttcactcgc cgaagccatg gagtattatt taccgcgcct gcgcaagcag
     4561 atcgacggca aaaagcctta tgccatcgtc caggcggaag acgaaatcgc ctctgccggt
     4621 atgatcgtcg gcgccggttg ggccggcgct aggtcaatga cctcaacctc ggggccaggc
     4681 gtgtcgctga tgaacgagtc catcggtttg gcttactacg ccgagattcc ttgcgtattt
     4741 ttcatcattc agcgcggcgg accgtccacg ggcctgccga cgcgcacaca acaggccgat
     4801 atttcactga tgtatggcgc ctctcatggt gatacccgtc atattattct gattccccat
     4861 gacatgaact cctgcttcga tctcgcgcgc cagagtttca attacgcc
//
LOCUS       DASQKS010000586         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_753483, whole genome shotgun sequence.
ACCESSION   DASQKS010000586 DASQKS010000000
VERSION     DASQKS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753483"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VE170_14950"
     CDS             complement(<1..297)
                     /locus_tag="VE170_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16793.1"
                     /translation="MVAASNSPVTVITGGGSGIGQATALRLARNGDRVCICDLNADAV
                     NATAERISADGGSVFTGVVDVRDQAQVSAWIARVIEKYGQIDCLFSNAGVNARAL"
     gene            complement(291..1013)
                     /locus_tag="VE170_14955"
     CDS             complement(291..1013)
                     /locus_tag="VE170_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16794.1"
                     /translation="MTNAIEHQGRVAVITGGSGGIGGAIAARLTAEGAVVHVLDVNPG
                     ADDTRFHAVDVGDEASVNQVIDAIGAAHGAIDYLVCCAGIYQPQPFLELTLDAWRQTL
                     QINLTGMFLSCRAALKYMTPRRFGRIVLFSSMAARTGGKNVAHYAASKGGILGLARAL
                     AVETAADNIRVNTISPVITDTAMPRAVVSDEYMQSRKQHIPLGRIGEVPDMVEACLFL
                     LSDDSSYIVGQDLRVNGGSTLW"
     gene            complement(1050..2072)
                     /locus_tag="VE170_14960"
     CDS             complement(1050..2072)
                     /locus_tag="VE170_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16795.1"
                     /translation="MRKFSWWTIAAALGLWFSVQASAQESFYKGKTVRILVGGSAGGG
                     YDTYTRTIARHLGKHIPGNPSFVVENMTGAGSLIAANYTYKIARPDGLTIGHFIGGLM
                     LQQVMGKPGIEFEGQRFEYLGVPAQDSLVLGVTKAAGITSVEQWLATKTTLKFGGVGP
                     GAGTDDVPKVVRATVGVPIQLISGYKGTADIRLAMAAGELHGVTNSWESFKSGWSKEI
                     ESGSVLLLLQTLPQRHPELPNVPAIHDFVKNDDDRKLVQTGIFDYSAIARPYALPPKT
                     PKDRVRLLRKGLTDTYKDPEFIADARRARLDMNPLTGEELEKVVARVYQLDKALVEKL
                     KDILLK"
     gene            2191..3177
                     /locus_tag="VE170_14965"
     CDS             2191..3177
                     /locus_tag="VE170_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ16796.1"
                     /translation="MQYRFLGKERLKVSAIGYGCPPFQGKLSDADERQAIAVLERAID
                     IGMNFIDTADHNNGNNEEILAKLLPGRRRDVVLTSKFGNLRGQPWADGREVEGKPGYV
                     AWACDNSLRRLQTEYIDLYYVHRVDPKVPIEETVGAMSRLVEQGKVRHLGLSEAGPET
                     LRRANAVHPITAVQSEYSLWTRDYERNTIPICRELGIGYVAYYALGRGFLSGKWNDFA
                     DLGEKEAKRRGPRFHAGNFQHNISLLKQLEAIARLKQCTVAQLALAWILHQGDFFVPI
                     PGTYKIANLEANAAAADISLTAEELAEIDRIFPLGAAAGGRHDYDRSKELNI"
     gene            3234..>4141
                     /locus_tag="VE170_14970"
     CDS             3234..>4141
                     /locus_tag="VE170_14970"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16797.1"
                     /translation="MRVLITGCGLIGAYTAKELVDRGDEVTFFDLQPNLSYLQKVLGR
                     ELPIIRGDIRELPALIEAMQSTRAEVVIHTAGLQGNRTPYFGFQVNLIGTLNVAEAVR
                     LTGVRRLIHASSQGVYDLTDPPIPIHEEARRDGGARVYQGGKAACEEVLTAYAATYKF
                     ELALPRFASVYGYGPYTGGGNVGMDMYAMLQAALAGKPAPMGPGIADANDFVYAKDIA
                     QGLRLLTSAASLPHQAYNLGTGVAATIADIQGGLQKVLGDVRFTRAQSSRPRPALDIS
                     RARLDLGYEPKFAVEAGLRDFIQEMGR"
BASE COUNT          826 a         1154 c         1301 g          860 t
ORIGIN      
        1 cagcgcgcgg gcgttgacgc cggcgttgct gaatagacaa tcgatttggc cgtatttttc
       61 gatcacccga gcgatccagg cgctgacttg cgcttgatcg cgcacgtcga caacgccggt
      121 aaatacactg ccgccgtcgg cgctaattcg ctcggccgtc gcgttgaccg cgtcggcatt
      181 taaatcgcaa atacagactc gatcgccatt gcgcgccaat cgcagcgcgg tcgcctgtcc
      241 gatgccgctg cccccgccgg tgatgaccgt gacgggcgaa ttcgacgcgg ctaccataga
      301 gtcgaccctc cgttgacgcg cagatcttgg ccgacgatgt aagagctgtc gtcgcttaac
      361 aaaaacaggc aggcttcgac catgtccggg acctcgccga tgcggccgag cgggatatgc
      421 tgcttgcgcg actgcatgta ttcgtccgac accacggcgc gcggcatcgc tgtatcggtg
      481 attaccggcg agatcgtgtt gacgcggatg ttgtcggcgg cggtttcgac ggcgagtgcg
      541 cgggcgaggc cgagtatgcc gcctttggac gccgcgtagt gggcgacatt cttgccgccg
      601 gtgcgcgcgg ccatggacga gaacaggacg atgcggccga accggcgcgg ggtcatgtat
      661 ttcaacgccg cgcggcagga gagaaacata ccggttaaat taatttgcag cgtttgacgc
      721 caagcgtcga gagtgagttc gagaaagggt tgcggctggt aaatgccggc gcagcagact
      781 aaataatcga tggcgccatg ggcggcgccg atggcgtcga ttacttgatt gaccgaagct
      841 tcatcgccga catcgaccgc atggaagcgc gtgtcgtcag cgcctggatt gacatcgagg
      901 acatggacaa cggcgccctc ggcggttaaa cgtgccgcga tggcgccgcc gatgccgccg
      961 ctgcctccag tgatgaccgc gacgcggcct tgatgttcaa tggcattggt catggtcgtt
     1021 tgttctgcgg caatgaggtt gtcaccggat tatttcagca gtatgtcttt gagcttttcc
     1081 accagcgctt tgtcgagttg ataaacccgc gctacgactt tttccaactc ctctccagta
     1141 agagggttca tgtcgagacg ggcgcgccgc gcgtcggcga taaactccgg atctttatag
     1201 gtatcggtga gtcccttgcg cagcagccgg acccgatctt tcggggtttt cggcggcaat
     1261 gcgtaggggc gggcgatggc gctgtaatcg aagatgccgg tctgcaccag tttgcgatca
     1321 tcgtcgttct tgacgaaatc atggatcgcc ggaacgttgg gtagttcggg atggcgctgg
     1381 ggcagtgtct gcagcagcaa gagcaccgaa ccggattcga tctctttgct ccatcccgat
     1441 ttgaacgatt cccatgaatt ggtcacgccg tggagctcgc cggcggccat cgccaggcgg
     1501 atgtcggcgg tgcctttata accgctgatc agttgaatcg gcacaccgac ggtggcgcga
     1561 actactttgg ggacatcgtc ggtgcccgcg ccggggccga caccgccaaa ttttaacgtc
     1621 gtcttggtcg cgagccattg ttcgacgctg gtgatgccgg cggccttggt gacgccgagc
     1681 actagcgagt cctgtgccgg cacgcctaaa tattcaaacc gttgtccctc gaactcgatt
     1741 cccggtttgc ccatgacctg ttgcagcatc aggccgccga taaagtggcc gatggtcaac
     1801 ccgtccggcc tggcgatctt gtaggtgtaa ttggcggcta tcaaactgcc ggcaccggtc
     1861 atgttttcga cgacgaagct ggggttgccg ggaatatgct tgccgaggtg acgggcgatg
     1921 gtgcgcgtgt aggtatcgta gccgccgccg gcggagccgc cgaccaggat gcgcacggtt
     1981 ttgcctttat agaaggactc ctgggcagat gcctggacag agaaccacag tccgagtgcg
     2041 gcggcaatcg tccaccagga aaattttctc atcggcaaaa ctcctcccca tttacctcaa
     2101 gcgctgggcg atagatagca aaaattcgcc atgttgcaac ttgaatcgct atgccgttgt
     2161 ggaccattat ttcgccggga ggggtttcac atgcaatacc gttttctcgg caaagagcgt
     2221 ttgaaagttt ccgccattgg ctatggctgc ccgccgtttc aaggcaaatt gagcgatgcc
     2281 gacgaacgtc aggcgatcgc cgtgcttgaa cgcgccatcg acatcggcat gaatttcatc
     2341 gacaccgccg atcacaataa cggcaacaac gaagagatcc tcgcaaagtt gctacccggc
     2401 cggcgccgcg atgtcgtgct gacttcgaaa ttcggcaacc tgcgcggcca gccgtgggcg
     2461 gacggccgag aagtcgaagg caaaccgggg tatgtggcgt gggcgtgcga caactcgttg
     2521 cgccggctgc aaaccgagta catcgatctc tactatgttc atcgcgtcga tcctaaagtg
     2581 ccgatcgaag aaaccgtcgg cgcgatgagt cggctggtcg agcaaggtaa agtgcgacat
     2641 ctcggcttga gcgaggccgg acctgagacc ctgcggcgcg ccaacgcggt gcatccgatc
     2701 acagcggtgc aaagcgagta ttcactgtgg acgcgtgatt acgagcgcaa taccattccg
     2761 atctgccgcg agttgggcat cggctatgtc gcttactatg cattgggccg gggattctta
     2821 agcggcaagt ggaacgactt cgccgatctc ggcgaaaagg aggccaagcg gcgcgggccg
     2881 cgcttccacg ccgggaattt ccaacacaat atcagtctgc tcaaacaact ggaggcgatc
     2941 gcgaggttga agcaatgcac ggtggcgcag ttggcactgg cgtggattct tcatcaaggt
     3001 gatttcttcg tgccgattcc cggcacatac aaaatcgcca atcttgaagc taatgcggcg
     3061 gcagcggata tttcattgac ggcggaagag ttggcagaga tcgaccggat ctttccgctg
     3121 ggcgcggccg cgggcgggcg gcatgattac gaccgatcta aagaattgaa catctaaaaa
     3181 aattcgttca agggttcaag cgttcaaggt tcaagggttc ggaggaagat gccatgcgag
     3241 tcttgattac cggttgtggt ttgatcggtg cctacacggc gaaagagtta gtcgaccgcg
     3301 gcgatgaagt gacgttcttc gatctgcaac cgaatctttc ttacctgcaa aaagttctcg
     3361 gccgggagct gccgattatt cgcggcgata ttcgcgagct gccggcgctg atcgaagcca
     3421 tgcagagcac gcgcgcggaa gtcgtcattc acaccgccgg attacagggc aatcggaccc
     3481 cgtactttgg ctttcaagta aacttgatcg gcacgttgaa tgtcgccgag gcggtgcgac
     3541 tgaccggcgt gcgccgcttg attcatgcca gcagccaggg tgtttacgat ctcaccgacc
     3601 cgccaatacc gattcacgag gaagcgcggc gcgacggcgg cgcgcgagtc tatcagggcg
     3661 gtaaagcggc gtgcgaggaa gtgctgaccg cctacgcggc gacgtataaa ttcgagctgg
     3721 cgctgccgcg cttcgcgtcg gtttacggct acggtcctta caccggcggc ggtaatgtcg
     3781 gcatggacat gtacgccatg ttgcaagcgg cgctggccgg caagcccgcg ccgatggggc
     3841 cagggatcgc ggacgccaac gattttgtct atgcgaagga tatcgcccaa gggctgcgct
     3901 tgctcaccag cgcggcgagc ttgccgcacc aagcgtataa tctcggcacc ggcgtcgccg
     3961 cgacgatcgc ggatattcaa ggcgggctgc aaaaggttct cggtgatgtg cgcttcacgc
     4021 gcgcgcagtc ttcacggccg cggccggcgc tggatatttc tcgagcccgg cttgatttgg
     4081 gttatgagcc gaagttcgct gtcgaagcgg ggttgcggga ctttatacaa gagatggggc
     4141 g
//
LOCUS       DASQKS010000587         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_754070, whole genome shotgun sequence.
ACCESSION   DASQKS010000587 DASQKS010000000
VERSION     DASQKS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754070"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..502)
                     /locus_tag="VE170_14975"
     CDS             complement(128..502)
                     /locus_tag="VE170_14975"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYJ16798.1"
                     /translation="MAQTAKDAFEKTALGAQNKAYDLIAQMDPEYFEKLKGLYVDGTF
                     GREGALPRKTKELIMVGICCALNRPRGVKLHSERALTLGAAPREVLEAVEVAAIPGGM
                     PGLWLGVETLNDILKAKGIEFK"
     gene            complement(654..2336)
                     /locus_tag="VE170_14980"
     CDS             complement(654..2336)
                     /locus_tag="VE170_14980"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ16799.1"
                     /translation="MSAETIASQAYLEVAAAHGVEYIFGLPGTSGQEFIGTIADQEKI
                     RFILAMHETCVVSMADGHARVTGRPQLAQVSTLPGSANSVGALYDAYRDRSPVIVTST
                     NVDTRIDGRDSHTEGKNLVEMTKQFTKWSAEVHRADRIPEYLNRAFKVASSAPTGPVY
                     LALPSNLLGESISVENPDAGRSRVSPRIAGDPEAFADAAKMLAHAKRPLIVAGSGIAK
                     AGAIEDLIKFAEMVAAPVVMEPRYSFLSFPTTHLQSFQIAERQPSFDLPMWGEPDVIV
                     AIGCRLIREYRYIPEPVIKSSTKIVHIEEDPWEIGKVFPVDIGIVADAGSALQSLIES
                     YAKVEPASGVKAERMECLRKAKDQATGEIDSRVSKGWDATPINAARVARTMDRLIDKH
                     ALIVNESPTSKDLLLSNFQFSLGRDYFSNSSGGYLGWGLGAAIGAKLAAPNRRVVACL
                     GDGSTMFGLQGLWTLARYRIPLVVIVFNNRAYMAVKNQFRGTEERIRIAADMGAEITA
                     PEINFARLAETFGIHGERVEHPDAIEAALKRALDQTGPALVDIVIGQNTRKD"
BASE COUNT          441 a          655 c          718 g          546 t
ORIGIN      
        1 tattcgcttc gctcatagga atgggactcg ttcaaaaggt tcaacctttt tgctcggtgc
       61 aagccgttcg cgccatacgt acggttcaat gttcaacaat aagggcgatt catgaatcgc
      121 ccctacgcta tttaaattcg atgcctttgg ctttgagaat gtcgttcaag gtttcgacgc
      181 ccagccagag accgggcatg ccgccgggga tggcagcgac ttcgaccgct tccaagactt
      241 cgcgcggcgc cgcgcccaag gtcagcgctc tctcgctgtg gagcttgacg ccgcgcgggc
      301 ggttgagtgc gcagcagatg ccgaccatga tcagttcctt ggtcttgcgc ggcagtgcgc
      361 cttcgcggcc gaaggtgccg tcgacgtata ggcctttgag tttctcgaaa tactccgggt
      421 ccatttgcgc gatcaggtca taggccttgt tctgcgcgcc caatgcggtt ttttcaaatg
      481 catcttttgc agtttgagcc atgagtgcct ccttgattta cacggttgtg accacgacgg
      541 gcacgacgct gtgaacaatt tctgccgaag ctttcgtgtc attcatgaat ctgcggtgca
      601 tgagttcttt cattttcgcc ggctgatgcg cggtggcgca tggttagctt cgctcaatcc
      661 ttccgggtgt tctggccgat aacgatgtcg acgagtgctg gaccggtttg atctaaagcg
      721 cgtttgagcg ccgcttcgat ggcgtcggga tgttcgacgc gctcgccatg gatgccgaag
      781 gtttcagcga gcctggcgaa gttgatttcc ggagcggtga tctcggcgcc catgtcggcg
      841 gcgatgcgaa tgcgctcctc ggtgccgcga aactgatttt ttaccgccat ataggcgcga
      901 ttgttgaaga cgatgacgac caggggaata cggtatctgg ccagggtcca taatccttgc
      961 aagccgaaca tcgtgctgcc gtcgccgagg caggcgacaa cccgccgatt cggtgcggca
     1021 agcttggcgc caatcgcggc gcccaagccc caacccaagt atccgccgga tgaatttgaa
     1081 aaataatcgc gccccagtga aaactgaaag ttcgacagca aaagatcttt gctggtgggg
     1141 ctctcgttga cgattagcgc atgtttatcg atgagccggt ccatggtgcg cgccaccctt
     1201 gccgcgttga tcggcgtggc gtcccaacct ttgctgaccc ggctatcgat ttcgccggtg
     1261 gcttggtcct tggctttgcg tagacattcc atgcgctcgg ctttgactcc cgatgccggc
     1321 tccactttcg catagctttc gatcagtgac tggagcgcgc tcccagcgtc ggcgacaata
     1381 ccgatgtcga cgggaaatac tttcccgatt tcccaaggat cttcttcgat atgaacgatc
     1441 ttcgtcgaag atttgatgac cggttcggga atatagcggt attcgcggat cagtcggcat
     1501 ccgatggcga cgatcacgtc cggctcgccc cacataggta gatcgaacga aggttgccgc
     1561 tcggcgatct gaaaactttg aaggtgcgtg gttggaaagg acaagaaaga atagcgcggc
     1621 tccatcacca ccggggcggc gaccatctcg gcgaatttga tcagatcttc gatcgcaccg
     1681 gccttggcga tgccgctgcc ggcgacgatc agcggccgtt tggcgtgggc gagcattttg
     1741 gcggcgtcgg caaaggcttc gggatcgccg gcgatgcgcg gcgagacgcg ggatcggccg
     1801 gcgtcggggt tttcgacact gattgattcg ccgagcaggt tacttggcag cgcgagatag
     1861 accggaccgg tgggcgccga cgatgcgact ttgaaggcgc ggttcaaata ctcggggatg
     1921 cggtcggcgc gatggacttc ggcgctccat ttggtgaact gcttggtcat ctcgaccagg
     1981 tttttgccct cggtatgtga gtcgcggccg tcgatgcggg tgtcgacatt cgtcgaggtg
     2041 acgatcaccg gcgagcggtc gcgataggcg tcgtaaagcg cgcccaccga attggccgag
     2101 cccggcagcg tgcttacctg cgccagttgc ggccgccccg tgacccgggc gtggccgtcg
     2161 gccatggaga cgacgcaggt ctcgtgcatc gccaaaatga aacggatctt ttcctgatca
     2221 gcgatggtgc cgatgaactc ctggccgcta gtaccgggca gaccgaaaat atattcaacg
     2281 ccatgagcgg cggcgacttc caaataagcc tgggatgcga ttgtttctgc ggacattatt
     2341 ttctcagctc ctctttaaag
//
LOCUS       DASQKS010000588         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4123902, whole genome shotgun sequence.
ACCESSION   DASQKS010000588 DASQKS010000000
VERSION     DASQKS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123902"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VE170_14985"
     CDS             <1..622
                     /locus_tag="VE170_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16800.1"
                     /translation="EVVGLMGRNGMGKTTLIRTLLGLVPARRGEVRIDGKVMTNAAPH
                     RIARQGIAYVPEGRGIFPNLTVRENLLMAARPTTDGQTLWSLDRVLETFPRLADRLGH
                     GGQQLSGGEQQMLSIGRALMTNPRLLMLDEATEGLAPLIAREIWRILGTIRATGIAAI
                     IVDKNFAAVSAVTDKNVILVKGKIVFAGSSSELRSQPELMQQYLGI"
     gene            complement(711..1058)
                     /locus_tag="VE170_14990"
     CDS             complement(711..1058)
                     /locus_tag="VE170_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16801.1"
                     /translation="MARIRHLAIVSENRERMVKFYTEAFGLKVIDGVGPAIYLSDGYI
                     NLAIIEKRPHYKEGLYHFGMELDSIEALRPVAKELGAQTEVQKRPPNREAEYRIADPD
                     GNLIDLSEHGWPV"
     gene            complement(1158..1400)
                     /locus_tag="VE170_14995"
     CDS             complement(1158..1400)
                     /locus_tag="VE170_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16802.1"
                     /translation="MAKIRAVVVEGDRERGYKRVQVIFGTNSFIEITENDGRVMCLLG
                     AHDGGVQADGSEAKGQFSQFVHELMERHPGNVWQEE"
     gene            complement(1603..2715)
                     /locus_tag="VE170_15000"
     CDS             complement(1603..2715)
                     /locus_tag="VE170_15000"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16803.1"
                     /translation="MKHGFKTFDSDMHVYDAANLYENYMNAQWGERIPKGQRNGKHGR
                     VEFKLGSGTTLRAITDVIDHGQQQVAERYVFAAARDYDPVSQLEAMDIEGLDVAVLFR
                     TSPLHCDETLEAAYANDLCKAWNSWIADFCKQDPARLKASALITLHDVDLAVEEARRA
                     VNGLGIRALSLCPEPINGKRIHDRYFDPLWAEIEKLGVALCFHPPARPKQEQVANKFF
                     GHPNASIVALALRNPVELIQAISCFCAGGVLEKFPNLRVAFLEGNCAWLPWLLYRLDE
                     RAKLHGPLADVPLSHRPSEYFLRQCFISMDPDEALVTDVIKRIGDETILISTDYPHID
                     AHFPHALDEFFEIKDIGDDSRRKILWDNCARLYGLG"
     gene            complement(2784..>3797)
                     /locus_tag="VE170_15005"
     CDS             complement(2784..>3797)
                     /locus_tag="VE170_15005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ16804.1"
                     /translation="VRVFTWLVLSLMVLPVGAQESYYRGKTVTFLVGAPPGGGFDTMT
                     RIVARHLGRNIPGNPIVVVQNMPGAGTVIAANHLYNRSKPDGLTIGVWVGSLTLQKML
                     GGTGLEFDPLKFEWIGVPVQQTMLCAFTKASGINSMEKWQAAKTPLKMGGQVPGTRPD
                     DISTILKAALNLPIQLVQGYKGTADITMAAEAGELAGQCTSWQGLKSSWRRQYEAGDV
                     HVVLQTNRRSIPELTHVPLAINYAKTDEAKQLIEVGIHDLNAITLAYSAPPGTPKDRV
                     QTLRNAVQATLKDHAFLADAKTAQQEVDPLTGEELATTIANFRKISPAVLKRLAELLL
                     PKK"
BASE COUNT          792 a         1130 c         1025 g          850 t
ORIGIN      
        1 tgaagtggtt ggcttgatgg gtcgtaacgg catgggcaag accacgctga ttcgcacctt
       61 gctcggactg gtgcctgcgc gccgcggcga ggtgcgtatc gacggcaagg ttatgaccaa
      121 cgccgcgccg caccggatcg cccgccaggg catcgcctat gttcccgagg ggcgcggcat
      181 ttttcccaat ctaacggtgc gggaaaatct ccttatggcg gcgcgcccga caaccgacgg
      241 ccaaactctc tggagcttgg accgcgtgct cgaaaccttt ccgcgcctag ccgaccggct
      301 tggccacgga ggccaacagc tttccggcgg cgaacaacaa atgctgtcca tcggccgcgc
      361 gctgatgacc aatccaagac tcttgatgct cgacgaagcg accgaaggtt tggcgccgct
      421 catcgcccgt gaaatctggc gcatcctcgg cacgatccgc gccaccggca tcgccgccat
      481 catcgtcgat aaaaacttcg ccgccgtatc ggcggtcacg gacaaaaacg tgatattggt
      541 taaaggcaag atcgtcttcg cgggaagcag cagcgagttg agatctcaac ccgaactcat
      601 gcaacagtat ctgggcatct aaccgcggct tgatataata caattacaag ttggtatcgc
      661 ggcccggttt gagacgtgca aatcgaaact cgaaaacttg gcagcctgct ctaaaccggc
      721 caaccatgct cggaaagatc aatcaggttg ccgtcgggat cggcgatccg atattccgct
      781 tctcggttgg gcggccgttt ctgcacctcg gtttgcgcac ccagctcttt ggcgaccggc
      841 cgcaatgcct cgatgctgtc gagctccatg ccaaaatgat aaagcccttc tttgtaatgc
      901 ggccgttttt caataatcgc cagattgata tagccgtcgg ataagtaaat cgccggaccg
      961 acgccgtcga tgactttcag accgaaagcc tcggtgtaga acttcaccat tcgctcgcgg
     1021 ttctcactga caattgctag atgccggatc cttgccatga tttttctcct ttggctaaat
     1081 taattgtaga aacgatcgct gcacaccgcc tggttccagc accgattcgt tgccaagtcg
     1141 ttgatgctcc taaccggcta ttcttcttgc cacacgtttc ccggatggcg ctccatcaac
     1201 tcatgaacga actgcgagaa ttgtcccttg gcttctgagc cgtccgcttg aacaccgccg
     1261 tcgtgggcgc ccaagagaca catgacacgg ccgtcgttct cggtgatctc gatgaaagaa
     1321 ttggtaccga agataacttg aactcttttg taaccgcgct cgcggtctcc ttcgacaacg
     1381 acggccctga ttttcgccat gacctacctc ccgaaatgca ttggttcgct tctctatgct
     1441 tcccgttcgc accagtcgcc agaaattatt caaacaacac ggaccgtttt ggcaatcacg
     1501 aaaaactcgc cgagaaataa ctaagcgggc gggtttcaaa cccgccccaa cgtatcggat
     1561 tctttttaaa tgcgcccttt gcgttctttg cggctaatcg tcttaaccca gcccatatag
     1621 ccgcgcgcaa ttgtcccaaa gaatttttcg tcggctgtca tcgccgatgt ctttgatctc
     1681 gaagaattca tccagagcgt gggggaaatg ggcgtcgata tgcgggtaat cggtggatat
     1741 caatatcgtc tcgtcgccga tccttttgat cacatcggtt accagtgcct cgtcggggtc
     1801 catggagata aaacactgcc gcaagaaata ttcgctcggt cggtgcgata gcggcacgtc
     1861 ggccaagggt ccgtgcaact tagcccgttc gtcgagtcgg tagagcagcc agggcagcca
     1921 agcacagttg ccttcgagaa aagccacgcg cagattggga aacttctcga gcacgccgcc
     1981 ggcgcagaag caggatattg cttgaatcaa ctccacgggg ttgcgcagcg ctagggcgac
     2041 gatgctggcg tttggatggc cgaaaaattt gttcgccacc tgctcctgct tcggccgcgc
     2101 cggtggatga aaacatagcg cgacgccaag cttttcgatc tcagcccaga gcggatcgaa
     2161 gtagcggtca tggatgcgct tgccgttgat cggctcgggg cataacgaca aggcgcggat
     2221 gccaagcccg ttgaccgccc ggcgcgcctc ttcgacggcc aggtcgacat catgcagcgt
     2281 gatcaacgcc gaagccttca agcgcgccgg atcctgcttg cagaagtcgg cgatccaact
     2341 gttccatgct ttgcagagat cgttggcgta ggctgcttcc aaggtttcat cgcagtgcag
     2401 cggcgaggtg cgaaacaaga ccgccacgtc caacccctcg atgtccatgg cctcgagttg
     2461 cgacactggg tcgtagtccc gcgccgcggc gaaaacataa cgctcggcga cctgctgctg
     2521 accgtggtcg atgacgtcgg tgatcgcccg cagcgtcgta ccgctgccga gcttgaactc
     2581 gacccggcca tgcttgccat tgcgttgccc cttgggtatg cgctcgcccc attgggcatt
     2641 catgtaattc tcgtaaagat tcgccgcgtc gtaaacatgc atgtcgctat cgaaagtctt
     2701 gaatccatgc ttcatcggtt catctcccgc tccaggtcag ggattccgaa aacgttttta
     2761 acggcttgat aatggcgaat tcactatttt ttcggcaaca acagctcggc caacctcttt
     2821 aataccgctg gtgaaatctt tctaaaattg gcgatcgtcg tggccaactc ctcgccggtc
     2881 aacggatcga cttcctgctg agctgtcttg gcatcggcca ggaaggcgtg atccttcagt
     2941 gttgcctgca cggcgttgcg caatgtttga actctgtcct tgggtgttcc cggcggcgcc
     3001 gaataagcaa gggtaatcgc gttcaaatcg tggatgccga cctcgatcag ttgtttggcc
     3061 tcatcggttt tggcgtaatt gatcgccaat ggcacatggg tcaattcagg aatcgacctg
     3121 cggttggttt gcagaaccac atggacatct ccggcctcat actggcggcg ccagctgctt
     3181 tttaagccct gccagctggt gcactgcccc gccagctctc ccgcctccgc ggccatggta
     3241 atgtccgcgg tgcctttgta gccctggacc aactgaatcg gcagattgag cgcggctttg
     3301 agaatcgtcg agatgtcgtc cggccgagtg cccggcactt ggccacccat cttaagcggt
     3361 gttttcgccg cctgccattt ttccatgctg ttgataccgc tagccttggt aaacgcgcac
     3421 agcatggtct gctgcaccgg cacgccgatc cattcgaact tgagcggatc gaactccaat
     3481 ccggtgccac ccagcatttt ttgcaaggtc aaactgccga cccatacgcc gatggtcaaa
     3541 ccgtcgggct tgctgcgatt gtaaagatga ttggccgcga tcacggtgcc ggcgccgggc
     3601 atattttgca ccacgacaat cggattgccg ggaatattgc gccccagatg gcgcgcgacg
     3661 atgcgcgtca tggtgtcgaa accgccgccc ggcggcgcgc caacgagaaa ggtcaccgtc
     3721 ttgccgcggt agtaagattc ctgagcgccg accgggagaa ccatcaacga caataccagc
     3781 caagtgaaca cacgcac
//
LOCUS       DASQKS010000589         9433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2449608, whole genome shotgun sequence.
ACCESSION   DASQKS010000589 DASQKS010000000
VERSION     DASQKS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449608"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VE170_15010"
     CDS             <1..750
                     /locus_tag="VE170_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16805.1"
                     /translation="ALITGAGRNIGKSMAKLFAAEGAKIVVAEMHEGRGQAVVDEISK
                     SGGQAALVLGDVSKSSDVQRMVKDAAAKFGGIDILINNAALTDHANILDSAEEEFDKV
                     IAVSLKGPYLMTKYVALEMKNQGRGGKVLNFGSTSGLVGRYDGIAYAAAKAGVINMTR
                     AMAVQLAEYGIRVNCIVPNRSGSPVGYDDDVAGSRTFQNLAGRLGTADDQAKAALFLV
                     SDDSSFIIGHPLIVDGGVMATANFPREQFQK"
     gene            complement(859..2112)
                     /locus_tag="VE170_15015"
     CDS             complement(859..2112)
                     /locus_tag="VE170_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16806.1"
                     /translation="MHLLSRILTILTYLLPFALVAALYLAIDRHPTVRRAAEITPGNI
                     QSARQILAQNDPLKPAYRTRQSLTLNQQDLDTAANYLAYFYANGGARLTLRNGKIDLT
                     ASLRPPKLPVIFYFNIGAILSSSGSSLEFEELRVGQLPLPGIVGDGLIKLIGARLIGT
                     EAYDLVNNAIKRVEIRNGQIVVGYQWPAASTGKLSAALISAVDQERLRVYQERLALLN
                     SSPQKRNVPLTELSIALFELATFRSQQLSAVDENRAALVVLAFYVNGKSLGRLLPAAD
                     NWPRPTEQVVTLNGRDDFAKHFIASAALAASAGSPLANATALYKEITDSHAGSGFSFD
                     DIAADHAGTRFGEHAAGITSAKKLQQRLTSGITEKAIMPATADLPEAMPAAEFKRRFG
                     GANGVEYKKLMAEIERRVEALPLYQ"
     gene            2533..3534
                     /locus_tag="VE170_15020"
     CDS             2533..3534
                     /locus_tag="VE170_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16807.1"
                     /translation="MADLLEVKNLQTSFFTPEGEVRAIDGISFEIDESKTLGLVGESG
                     CGKSVTSLSIMRLIPSPPGKIVGGEIVYRGKDLLKLGNEQMRKIRGNEISMIFQEPMT
                     SLNPVFTIGNQIGEAIKLHQGLNNQATRAKTIEMLRLVKIADPESRVDAYPHQLSGGM
                     RQRVMIAMALSCNPSLLIADEPTTALDVTIQAQILDLMKELQQKIGMALLLITHDLGV
                     VAEQADDVAIMYAGKIVERSNTREIFTKPFHPYTVGLLNSLPRTGGAKKHRLDAIPGV
                     VPNPLNLPSGCRFRDRCPRAADLCAQTEPELVEKAPGHTVACHFPHSETRAGVNADV"
     gene            3613..4590
                     /locus_tag="VE170_15025"
     CDS             3613..4590
                     /locus_tag="VE170_15025"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HYJ16808.1"
                     /translation="MSLLEIRDLKKYFPVGEGLFSRGKGAVKAVDGVNLSIEEGETLG
                     LVGESGCGKSTLGRTILRLIEPTGGEINFLGKNLLSMSQRELRDARREMQIIFQDPYA
                     SLNPRMRVGDIVGEGLEIHKLAKGKAKRERVMELLHQVGLRVDHFDRYPHEFSGGQRQ
                     RIGIARALAVSPKFIVCDEPVSSLDVSIQAQIINLLQELQEKMHLTYLFISHDLRVVE
                     HISHRVAIMYLGKVVEIAKSDTIYRDAKHPYTRALLSAVPIPDMNKKKERVVLEGDVP
                     SPVNPPSGCTFHPRCAYREAVCSQTEPALEFSADGHGVSCHVFGPGARR"
     gene            4751..8119
                     /locus_tag="VE170_15030"
     CDS             4751..8119
                     /locus_tag="VE170_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HYJ16809.1"
                     /translation="MAISSLGYREKLSALTGVFLLAAMAFSGIAASASTAASEPDGAT
                     MRSSENKRAQRLAVNLPLYFIENQGQLDRRAAYYVHGRDKVIYFTARGLTIVLHSQRP
                     QTAAVIAVASAGDDPTPIAAARWALQLDFVDADPAVRPSGENPSTALMSYFKGRPEEW
                     QPAAKTFSRLVYSDLWPGIDLVFTGAANQLKYLFVVKPGADPSAIKLRYRGADSVAIN
                     RAGHLEVRTPLGDLKDDRPTAYQDGISASADVEVAYSLLPRADDESYHYGFKLAAYDR
                     SVPLIIDPAVFVYAGFIGGSGDDRGNAIAVDSDGNAYITGETNSLEATFPEVGGPDRT
                     FNGTVDAFVAKVNAAGTALIYAGFLGGSGNDRGKAIAVDSAGNAYITGDTTSTQSSFP
                     VTVGPDLTQNGGTDAFVAKVNASGTALSYAGFIGGSGSDQGNAIAVDSSNRAYVAGAT
                     NSLGTSFPDGDGFGALASFDTTESGILDGFVVRVAANGTALEYAGYIGGSGIDQVFGI
                     AVDAAAAAYLTGDTNSSAATFPVTGALDAVHNGGVDAFVAKVNPTGTALVYAGFLGGV
                     GDDQGKGIALLPGCALNCEAYVAGETNSTQATFPVTVGPDLTHNGGIDGFVAKVNASG
                     SALVYAGYVGGSNTDRANGIAVDSSGIAHIVGETDSTQVTFPNGNGFGLLTSSDNTHN
                     GAIDGFVVGVNAAGTALLHASYIGGTGIDRGKGIASDDGGDIYVTGESEANSLSFPIG
                     GGTGPLATIDSSRNGGVDAFAIKICVTVCHDLQLTQVDSPDPVRVGDNLTYTVTVTNN
                     GPDSAANVVLTDLLPATATLVSATPSSGTCNGTTTVTCDLGNLADGASATVVVVVTTT
                     AKGTLKNVVTVDADDSDTSARNNRDEELTVVTFANLTVQALTAPSAVLPGATIDIQDT
                     TQNSGTVDANSVTVTRIYLSSDSRKDAGDIILDARTVPALAKKQSNTAVTPVTIPGGT
                     ALGRYFIIAAADDDTAVVETNERNLRSRRITVTRPDLSVSSLRSPASVIAGATLAIQD
                     TTRNDDLVEGGASTTKLYLSVDRLFDGGDVLLGSRAVPALGARATSAASTTVTIPLAT
                     APGRYFVIGVADGDANVVESDEANNARVRALTVK"
     gene            8276..8758
                     /gene="greA"
                     /locus_tag="VE170_15035"
     CDS             8276..8758
                     /gene="greA"
                     /locus_tag="VE170_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYJ16810.1"
                     /translation="MADASVPMTSRGFQFLTEELKRLKSVERPKNIRDIEEARDHGDL
                     SENAEFHAAKERQSLLDFQIREMEDKIARAQVIDISKLSGDKVVFGATVTLADGDSGD
                     KVVYQIVGDHEAEPKNGKISISSPIARALIGKSEGDEAQVHTPTGVRTFDILGVEYID
                     "
     gene            8954..>9433
                     /locus_tag="VE170_15040"
     CDS             8954..>9433
                     /locus_tag="VE170_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16811.1"
                     /translation="MAVPAGAEIAIEARVGFHGVFQLGRPFPLEVEVVNSGRPAEGTL
                     DVQVWKGGATKGGTPYPVKYRREVFLAAQSRKTIQFTVDPDFISRPLTISFSSASGNA
                     AREIDLRRNFSPAPVLLLVNDSGSLPPIALGPTAQSRLVALSLSELAADARALLGVSH
                     "
BASE COUNT         1950 a         2592 c         2844 g         2047 t
ORIGIN      
        1 gcgctgatta ccggggcggg ccgaaacatc ggcaagtcta tggcgaagct gttcgcggcc
       61 gaaggcgcga agattgtcgt tgccgagatg catgaggggc gcggccaagc cgtggtcgac
      121 gagatcagca aatccggcgg ccaagcggcg ctggtattgg gtgatgtatc caagtcgtcg
      181 gacgtgcagc gcatggtgaa agacgccgcg gccaagttcg gcggtatcga tattctcatc
      241 aacaacgcgg cgcttactga tcacgccaat attcttgaca gcgccgagga ggagttcgac
      301 aaggtcatcg ccgtcagttt gaaaggcccg tatttgatga cgaagtatgt cgccctggaa
      361 atgaagaacc aggggcgcgg cggcaaggtt ttgaatttcg gttcgacatc gggactggtc
      421 ggtcggtacg acggcatcgc ctacgccgcg gccaaagccg gcgttatcaa tatgacccgc
      481 gccatggccg tgcagttggc ggagtacggc attcgcgtca attgcatcgt gcccaaccgc
      541 tcgggttcgc cggtgggtta cgacgatgac gtggccggca gccggacatt tcagaatctc
      601 gccgggcgcc tcggcacggc ggacgatcag gccaaggcgg cgttgtttct ggtcagcgac
      661 gattcgagct ttatcatcgg ccatccgctg atcgtcgacg gcggcgtcat ggcgacggcg
      721 aatttcccca gagagcagtt tcaaaaataa aattaacctg ggtccaatcg ttcaaggctt
      781 tcaagccgtt caactcgtag ggcgaggctg cggtgctcat cgcgatgact ggcgtaccag
      841 ccaacgagaa gattgagttt actgatagag cggcagtgcc tccactcgcc gttcgatttc
      901 cgccatcagc tttttatatt cgactccgtt ggcaccgccg aagcggcgct tgaactcagc
      961 tgccggcatg gcttccggca gatcggcagt ggcaggcatg atggcttttt cggtaatgcc
     1021 gctcgtcaac cgttgctgta atttctttgc gctggtgatt ccggcggcat gctctccaaa
     1081 gcgcgtgccg gcatggtcgg cggcaatgtc gtcaaaggaa aagccgctgc cagcatggga
     1141 gtccgtgatt tctttataga gcgccgtagc gtttgcaagc ggactgccgg cactggcggc
     1201 gagcgcggcc gaggcgataa agtgtttggc gaagtcatca cggccgttga gcgtaaccac
     1261 ctgctcagtc ggccgcggcc agttatcggc cgccggtagt aacctgccga gagacttgcc
     1321 gttgacgtag aaggccagga cgacgagggc ggcgcgattc tcatccacgg cgctcaattg
     1381 ttgtgaccga aatgtcgcga gttcaaacag ggcgatggag agttcggtga gagggacgtt
     1441 tcgtttttgc ggcgaactgt taagtaacgc caagcgttct tgatagaccc gcagccgctc
     1501 ttgatccacc gctgaaatta gtgctgcgga gagtttgccc gttgacgcag ctggccattg
     1561 gtagccgacc actatttggc cgtttctgat ctcgactcgc ttgatggcat tattgaccag
     1621 gtcgtaggct tctgttccga tcagccgtgc accgatcagt ttgatcaatc catcgccgac
     1681 aattcccggc agtggcagtt ggccaacgcg tagttcctcg aattccagcg acgacccgct
     1741 gctggaaagg atcgcgccga tattgaaata gaaaatcacc ggtaatttcg gcggccgcag
     1801 actggcggtc aagtcgatct tgccgttacg cagggtaagc cgagcgccgc cgttggcgta
     1861 aaagtatgcc agatagttgg cggctgtatc gagatcttgc tggttaagcg tgagtgattg
     1921 gcgagttcgg tatgccggtt taagcgggtc attttgcgcc aggatctgtc gcgcactttg
     1981 aatattaccc ggggtaattt cggcggcacg cctgacggtc gggtggcggt cgattgccag
     2041 atatagggcc gcgaccaggg caaacggcag caagtaggta agtatggtga gaattctgct
     2101 tagtaggtgc atgacgggat gctcggaaat tctacgtcat gcgtcgacaa aagccaacta
     2161 agaaagcaag tgcgccggcg caaaagaaat ttccagcagg ctaggcgaga gggtttttcc
     2221 gttaacggac gatggcggga atctgcggcg tttggctcac ggcgtcgccg gcaaagactg
     2281 cggactgact ggggttatcg tcctttagct agatatctcc gcctcggtcg agttggccgt
     2341 cctgtggcgc gcaggcagtc tttactcgcg gcttcagcgg ggctggccgt atctgccgat
     2401 gactcatcat attggcaggg ctgggtttta atgctatggg agatgcaatt cacagtattt
     2461 tggacccgcg tatgttgaat cggttgcgcg gaggccgtac aacgtgagtt aagctaattt
     2521 caacctgtca acatggctga cctactcgag gttaaaaacc tccaaacctc ttttttcaca
     2581 ccggaaggcg aagtacgcgc catcgatggc attagcttcg agatcgacga gagcaagaca
     2641 ctcggactgg tcggcgagtc cggctgcggc aagagcgtca cttcattgtc gatcatgcgg
     2701 ctgattccgt cgccgccggg caaaattgtt ggcggcgaga tcgtctaccg cggcaaggat
     2761 ctgctcaagc tcggtaatga gcagatgcgc aagatccgcg gcaacgagat ctcgatgatt
     2821 ttccaggagc cgatgacctc gctcaatccg gtttttacca tcggtaatca gatcggagag
     2881 gcgatcaaac tgcatcaagg gctgaacaat caggccacta gggcgaaaac catcgaaatg
     2941 ctccggctgg tgaagatcgc cgaccccgaa tcccgcgtcg atgcctatcc gcatcagttg
     3001 agtggcggca tgcgccagcg ggtgatgatc gccatggcac tgtcgtgcaa tccgtcgctt
     3061 ttgatcgccg atgagccaac caccgccctc gatgtgacga ttcaggcaca gatcctcgat
     3121 ctgatgaaag agttgcagca gaagattggc atggctttgt tgttgatcac tcatgatctc
     3181 ggcgtggtcg cggagcaggc cgacgatgtc gcgattatgt acgccggcaa aatagtcgag
     3241 cgctcgaaca cgcgggagat tttcaccaag ccgtttcatc cttatacggt cgggctgctc
     3301 aactcgctgc cgcgcaccgg cggcgcgaaa aagcatcgct tggacgcgat tcccggtgtg
     3361 gtgcccaatc cgcttaattt acccagcggt tgccgcttcc gcgatcgctg cccaagggcc
     3421 gctgacctct gcgcccagac cgaaccagaa ttggtggaga aagcgccagg gcatacggtg
     3481 gcttgtcatt ttccgcattc cgagacgagg gcgggtgtta atgcggacgt ttagtcaaag
     3541 aggcaagatg gagaatggaa ggttgccgtc cgcgatcttc gatcgtctgt cctcgacatg
     3601 ggatatttgc cgatgagctt gctagaaatt cgcgacttaa agaaatactt cccggtcggc
     3661 gaaggattgt tctcgcgcgg caagggcgcg gtcaaggcag tcgatggcgt taatttgtcg
     3721 atcgaagagg gcgagacgct gggcttggtc ggcgaatcgg gttgcggcaa gtcgaccctc
     3781 gggcgcacca tcctgcgctt gatcgagccg accggcggcg agatcaattt tctcggcaag
     3841 aatttgctgt ccatgtcgca gcgcgagctg cgcgatgccc ggcgcgagat gcagattatc
     3901 tttcaagatc cctacgcctc gcttaatccg cgcatgcgcg tcggcgatat cgtcggcgag
     3961 gggttggaga ttcacaaact cgccaagggc aaagccaagc gcgagcgggt gatggagttg
     4021 ctccatcaag tgggtctgcg cgtagatcac ttcgaccgtt atccccacga gttcagcggc
     4081 ggccagcgcc agcgcatcgg catcgcccgc gctttggcag tcagtcccaa atttatcgtc
     4141 tgcgacgagc cggtgtcgtc cctcgatgtg tcgatccagg cgcaaatcat caatctgttg
     4201 caggaactgc aagaaaagat gcatttgacc tatcttttca tctcgcatga tctgcgcgtt
     4261 gtcgaacata tcagccatcg tgtagcgatc atgtacctcg gcaaagtcgt cgagatcgcc
     4321 aagagcgaca cgatttaccg cgacgccaag cacccgtaca cccgtgccct gctctccgcc
     4381 gtgccgattc ccgacatgaa caagaaaaaa gagcgcgtgg tgctcgaagg tgatgtccca
     4441 agtccggtca atccaccatc cggctgcacc ttccatccgc gctgcgccta tcgcgaagcc
     4501 gtgtgcagcc agaccgagcc ggcgctggag tttagcgccg acggccatgg cgtatcctgc
     4561 catgtgtttg ggccaggcgc gcggcgctga gccattgcac tgctattcgg gttccttaaa
     4621 gaagttcgat tcccgaccat tatcaatctt agtcagtgac ccaatatctc atgcatggta
     4681 ctctgttgcc tcgcgtcgcc aagtttttgc ggtggcgtga gttaacagga gtgctggagg
     4741 gtctgacgct atggcgatct caagcttggg ttatcgcgaa aagttgtctg cattgacagg
     4801 agtttttctc ctcgcggcaa tggctttttc cggaatcgcg gcttcggcca gcacggccgc
     4861 gtcggaaccc gatggcgcga ccatgaggtc gtctgaaaat aagcgagcgc aacggctggc
     4921 ggttaatctg ccgctctatt ttatcgaaaa tcaaggtcag ctcgatcggc gggcggcgta
     4981 ctatgtgcat ggacgcgaca aagtcattta tttcacggcc cgcggtttga ccatagttct
     5041 gcatagtcag cggccgcaaa ctgctgccgt tattgcggtt gccagcgcgg gcgatgatcc
     5101 cactcccatt gccgcggcgc gttgggcgct gcagcttgat tttgtcgatg ccgatcccgc
     5161 cgtccggccg agcggcgaaa atccttcaac ggcgctgatg agttatttca agggccgtcc
     5221 ggaggagtgg cagcccgccg caaaaacctt ctcccggttg gtttactcag acttatggcc
     5281 cggcatcgat ctggttttta ccggcgccgc caaccagctg aaatatcttt tcgtcgtgaa
     5341 gccgggagcg gatccctcag ccatcaaact gcgctaccga ggcgccgatt ccgtggcgat
     5401 aaaccgtgcc ggccacctgg aagtgcgcac gcctttaggc gatctcaaag atgaccggcc
     5461 gacggcatac caagacggga tttcggcaag cgccgacgta gaagttgcct acagcttgct
     5521 gccccgcgcg gatgacgaat cctaccacta tggctttaag ctcgccgcct atgatcgctc
     5581 ggttccctta atcatcgatc cggcagtatt tgtctatgca ggatttatcg gcggcagcgg
     5641 cgacgatcgc ggcaacgcga ttgctgtgga cagtgacggc aatgcttaca tcaccggcga
     5701 aaccaattcg ctcgaagcga catttcccga ggtcgggggc cctgaccgaa ctttcaacgg
     5761 gacggtcgac gcatttgtcg ccaaggtgaa tgccgcgggg acggcgttga tctacgcggg
     5821 atttctcggc ggctctggta acgaccgggg caaagccatc gccgtggata gcgcggggaa
     5881 tgcctatatc accggcgata caacctccac ccagagcagt ttccccgtga ccgtcggccc
     5941 tgacctaaca cagaatggcg gcacggatgc gttcgtcgcc aaggtgaatg cctccggcac
     6001 cgcgttgtcc tacgccggct ttatcggcgg tagcggcagc gatcagggca acgccatcgc
     6061 ggtggatagt tcaaaccggg cttatgtcgc cggggcgact aactccctgg gtacctcatt
     6121 cccggacggg gatggttttg gcgcgctggc gtcattcgat actaccgaaa gcggcatact
     6181 cgatggcttt gttgtcaggg tggcggccaa tggtacagca ttggaatacg ccggatacat
     6241 cggtggatcc ggcatcgatc aagtctttgg aatcgcagtg gatgccgcgg ccgcggccta
     6301 tctaaccggc gataccaatt ccagcgcagc gacctttccg gtcaccggcg ctctcgacgc
     6361 cgtgcacaac ggtggcgtcg atgcgtttgt ggcaaaagtt aatccgacgg gtacggcgtt
     6421 ggtttatgcc ggctttcttg gcggtgtcgg cgatgatcaa ggtaaaggta tcgccctcct
     6481 gccgggctgt gcgctcaact gcgaggctta tgttgccggt gagaccaatt cgacccaagc
     6541 tacttttccc gtcaccgtcg gtccggattt aactcacaat ggcggcattg atggctttgt
     6601 cgcgaaggta aacgcctccg gctcggcgct cgtttacgcg ggttacgtcg gcggaagcaa
     6661 caccgatcgg gcgaacggta tcgcggttga ctcaagcggc atcgcgcata tcgtcggcga
     6721 gaccgattcc acgcaagtaa cgtttcccaa tggcaatggt tttggtttgc ttacctccag
     6781 tgacaatacg cacaacggcg ccatcgacgg ctttgtcgtc ggcgtaaacg cagctggtac
     6841 ggctttactc catgcgagct acattggcgg cactggtatc gatcgcggca aaggcatcgc
     6901 tagcgatgac ggcggcgata tctatgtgac cggagaaagc gaggcgaatt cgctgtcttt
     6961 tcccatcggc ggcggtacag gtccgctggc gaccatagat agtagtcgca acggcggagt
     7021 cgatgctttc gcgataaaaa tctgcgtaac agtttgccac gatctccagc tcacccaggt
     7081 ggactccccc gacccggtca gagtcggcga taatctgacg tatacggtga cagtcaccaa
     7141 caacgggccg gatagcgcgg ccaatgtggt gttgaccgac ctattgccgg caacggcgac
     7201 gttggtttcc gcaactccca gcagtgggac ttgtaacggg acgactacgg ttacatgtga
     7261 tttgggcaac ctggcggacg gagccagcgc gacggtggtc gtcgtggtta cgactacggc
     7321 caagggaacg ttgaagaatg tcgttactgt ggatgccgac gacagcgata ctagcgctag
     7381 gaacaaccgg gacgaggagc tgacggttgt gacatttgcc aatctgacgg tgcaagcatt
     7441 gaccgcgcca tcggcggtat taccgggtgc gacaatcgat atacaggata ccactcagaa
     7501 ttcaggcacg gttgacgcca acagtgtcac ggtcacgcgg atttatcttt ccagcgatag
     7561 cagaaaagat gccggcgata taattctcga cgcgaggacg gtgccggcgt tggccaagaa
     7621 acaaagcaac acggcggtca ccccagtcac gataccggga ggcacggcgc tgggcaggta
     7681 tttcatcatc gccgcggccg acgatgacac ggcggttgtt gaaaccaacg agcgcaacct
     7741 aagaagccgc aggataacag taacgcggcc ggatctcagc gtttcctcac tgcgcagtcc
     7801 cgcttccgta attgcgggag ccaccctggc aattcaagat actactcgca acgacgacct
     7861 ggtcgaaggt ggcgccagca ctacgaagct ttatctttcg gtggacaggc tatttgacgg
     7921 cggtgatgtt ttacttggca gtcgggccgt gccggcattg ggggcgcgtg ccacaagcgc
     7981 ggcatccacc accgtgacga ttccgctggc aacggctcca ggccgctact tcgtgattgg
     8041 tgtggccgat ggcgatgcca atgtcgtaga atccgacgaa gccaataacg cgcgcgttcg
     8101 tgcgcttacg gtgaaataac cgattgctgt caactgaccc ttggatcggt tgatttagtc
     8161 actgtcgatg gtaaaatacc cggcagattt agcagctgga aaaatctaag atttttattc
     8221 aaggcctctc gggagagatt ccgaggggtt tttattttgt ctaggagttg acggtatggc
     8281 agatgcaagc gtcccgatga ctagccgagg attccagttt ctgacggagg agctgaagcg
     8341 cttgaagagc gtcgagcggc ccaagaacat ccgcgacatc gaagaggcgc gcgatcacgg
     8401 cgatctttcg gaaaacgccg agtttcatgc cgccaaggag cgccagtcgt tgcttgattt
     8461 ccaaattcgc gaaatggaag acaaaatcgc tcgcgctcag gtcatcgaca tttcgaaact
     8521 ctcgggcgac aaagtcgttt tcggtgcgac ggtgacgctg gccgatggcg atagtggcga
     8581 caaggtggta taccaaatcg tcggtgatca tgaggcggag ccgaagaacg gaaagatttc
     8641 gatcagttct cccatcgcac gggccttgat cggcaaaagc gagggggacg aagcgcaggt
     8701 ccacactccc acgggcgttc gcacgtttga tattctcggc gtggaatata tcgactaacc
     8761 gcttgtgcca gtggggtggc ctgtctggaa gggaaagatg cccttttcag ccgcacagtc
     8821 tgcccgcacc agaatctcac cgcgatgttc catcacgcgg ttttcttccg atcttttttt
     8881 cccagagcga gtttcttgta gcttacgttc tcgcggcgcg ccagcgattt tcgtttcgct
     8941 attgctagca ctcatggcgg ttccggccgg cgcggaaatt gccatcgaag cgcgagtcgg
     9001 tttccacggc gtgtttcaac tggggcgccc ctttcctttg gaagtcgaag tcgtcaattc
     9061 gggacgcccg gctgaaggca cgctcgatgt gcaggtatgg aagggtggcg ccaccaaagg
     9121 cggtacgccc tatccggtca agtaccggcg tgaagtattt ctcgccgcgc aatcgcgcaa
     9181 gacaatccaa tttaccgttg atccggattt catcagccgg ccgctgacca tttcattttc
     9241 cagtgctagt ggtaatgcgg cgcgcgagat cgatctgcgg cgcaattttt caccggcgcc
     9301 ggtgctgctg ttggtcaacg atagcggctc gctgccgccc atcgctctcg ggccgacggc
     9361 gcagagccgg ctggttgcgc tttccctgag cgaactcgct gccgacgcgc gggcgctgct
     9421 cggcgtttcc cat
//
LOCUS       DASQKS010000590         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4124712, whole genome shotgun sequence.
ACCESSION   DASQKS010000590 DASQKS010000000
VERSION     DASQKS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4124712"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VE170_15045"
     CDS             <1..881
                     /locus_tag="VE170_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16812.1"
                     /translation="FWTWLWGPVGLLLAMPLTVCVVVISKYVPPMQFVDVLLSDGSVL
                     EPDIVYYQRLLAADRAEAKAIVEAYIEENSAAQVYDGLMVPALGYAKRAWLQGNLTEA
                     EVQFVVQTTREILAEAKFAEPESAANGDVVETAPEPALPKLRIIGCPARDEYDELVLI
                     MLSQLLDHRRFDLQIVGAAALTSEVIARTAETNAPVICIASLSPDGGPHLRALCKRVR
                     QRFTEIKIVVGRLGNGAAHSDDLLAAGADKISATMIEFRDQLVQLSQIIEPEINRRQN
                     SPGEAETTHGDSETLM"
     gene            984..2909
                     /gene="treZ"
                     /locus_tag="VE170_15050"
     CDS             984..2909
                     /gene="treZ"
                     /locus_tag="VE170_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HYJ16813.1"
                     /translation="MNQRTDSGGPVPASPQSANRRLPIGAEVMATEGVHFRVWAPKCQ
                     RIEVVFAAAGGTPAVAPLELVAEDGGYFAGVASAARAGMLYRFRLNGSEQLVPDPASR
                     FQPDGPHGPSQIIEPGQFAWSDSGWRGAALQGQVLYELHIGTFTGEGTWRAAQAELDE
                     LKKLGITVLEVMPVNEFAGRFGWGYDGVDLFAPTHLYGKPDDFRRFVDRAHSLGIGVI
                     LDVVYNHLGPDGCYLKLFSEDYFSVRHTTDWGEAINYDGDNCQPVREFFRCNAGYWIA
                     EYHLDGLRLDATQNIYDDSTPHIVADIAAEVKNVAAPRATIVIAENESQDVKLVRPPS
                     AGGCGLDGMWNDDFHHSARVALTGEREAYYTDYLGSPQELISAVKWGFLYQGQWYTWQ
                     KQRRGTPTRGLKPSTFVTFLENHDQICNSGFGDRIHFSSHPGCYRALTALLLLGPGTP
                     LLFMGQEFGASSPFVFFADHKPELAKLVYKGRLEFVSQFTSLATPESQARIPDPAASQ
                     TFEACKLKLAEREEHKAIYQLHRDLLKLRHDDTVFSAQGAHGFDGAVLAPKVFVLRFF
                     GQEKEGDRLLVVNLDVDFIYDPAPEPLVAPPEKSKWQIIWSSESPVYGGRGVTPFTEN
                     WKIPGYAAIALTSVNEA"
     gene            2961..>3457
                     /locus_tag="VE170_15055"
     CDS             2961..>3457
                     /locus_tag="VE170_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HYJ16814.1"
                     /translation="MRVWPGKPYPLGATWDGKGVNFALFSEAATKVDLCLFDGVDASQ
                     EAQRLELTERTDQVWHAYLPEALPGQLYGYRVHGPYEPSQGHRFNPHKIVLDPYAKSI
                     GRELRWADEVFAYRMGDPEGDLTFDERDSAPYAPLATIIDPAFTWGDDKPPHTPWHKT
                     IIYEL"
BASE COUNT          745 a          921 c         1017 g          774 t
ORIGIN      
        1 cattctggac ctggctctgg ggtccggtag gactgctact cgccatgccg ctcaccgtct
       61 gtgttgtggt aatcagcaag tatgtgccgc caatgcaatt tgtcgacgtc ttgctgagcg
      121 atgggtcggt cctcgagccg gatatagttt attatcaacg tttgctcgcg gccgaccggg
      181 ccgaggcgaa ggcgatcgtc gaagcatata tcgaagaaaa ctccgccgcg caggtttacg
      241 acggcctaat ggtgccggcg ctcggttatg ccaaacgcgc ttggttgcag ggcaatttaa
      301 ccgaagccga agtacagttc gtggtgcaga cgacgcgcga gatcctggca gaggcgaaat
      361 tcgctgaacc agaatcagcc gctaatggcg atgtagtcga gaccgcgccg gagcctgctt
      421 tgcctaaact gcggataatc ggttgtcccg ccagagacga atatgacgaa ctcgtgctga
      481 tcatgctcag ccaattgctc gatcacaggc gtttcgattt gcagattgtc ggagccgcgg
      541 cgctgacctc cgaggtcata gcgcgaaccg cggagacaaa cgcgccggtg atctgtattg
      601 cctcgctttc gccggacgga gggccgcacc ttcgagcgct gtgcaagcgc gtccggcagc
      661 gctttaccga gatcaaaata gtcgttggcc gattgggtaa cggcgcggct catagcgatg
      721 atttattggc ggcgggcgcc gataaaatca gcgcaacaat gatcgagttt cgcgatcaac
      781 tggtccagct gagtcaaatt attgagcccg aaattaaccg tcgacaaaac tctcccggag
      841 aagcagaaac aactcatggc gattctgaaa cacttatgtg attctatttc tcacagttgc
      901 aacgcgcagt cagctaaggt gaatacagtc cggcattgcg ctaacgtaaa gtggcacagg
      961 cgttgcgctg cgggagcaga atcatgaatc aacgaaccga tagtggtggc ccggtgccag
     1021 cttcgcctca atccgcgaat agaagattgc ccatcggcgc cgaagtcatg gcgaccgagg
     1081 gcgttcattt ccgcgtgtgg gcgccaaaat gtcaacgcat cgaagttgtt tttgcagcgg
     1141 ccggtgggac gccggcggtg gctccgctgg agctggtggc ggaggatggc ggttatttcg
     1201 caggggtggc gagcgcggcc cgagccggta tgctctatcg ctttcgtctg aatggcagtg
     1261 agcaactcgt tcccgacccc gcatctcgat ttcaacctga cggacctcat ggcccgtcgc
     1321 agatcatcga gcccggccaa tttgcttgga gcgatagcgg ttggcgcggc gctgcgctgc
     1381 aaggtcaggt actttatgag cttcatatcg gcacgttcac aggcgaggga acctggcgcg
     1441 cggcgcaggc cgagctcgat gaactgaaga agctggggat tacggttctc gaagtaatgc
     1501 cggtcaatga atttgccgga cgatttggct ggggctatga cggcgtcgat ctatttgcgc
     1561 cgacgcatct gtacggcaag cccgatgatt tccgccgctt tgtcgatcgg gctcactctt
     1621 taggcatcgg agtgattctc gatgtggttt acaatcacct gggtcccgat ggttgttacc
     1681 tcaagctatt ttccgaagac tatttcagcg tcagacacac gaccgattgg ggcgaggcga
     1741 ttaactacga cggcgacaat tgccaaccgg tgcgcgaatt tttccgctgc aatgcgggct
     1801 actggatcgc cgaatatcat cttgacggcc tgcgcctcga cgcgacgcaa aatatttatg
     1861 acgattcgac gccgcatatt gtcgcggaca tagccgcgga ggtcaaaaac gttgcggcgc
     1921 cgcgcgcgac catcgttatc gccgagaatg agtctcaaga cgttaaattg gtgcgcccgc
     1981 caagcgccgg cggctgcggt ttggatggca tgtggaatga cgatttccat catagcgccc
     2041 gggttgccct caccggcgaa cgggaggcat attataccga ttacctgggc tcgcctcagg
     2101 aactgatttc agcggtcaag tggggatttt tatatcaagg gcagtggtac acatggcaaa
     2161 agcaacggcg cggcacgccg acccgcgggt taaaacccag cacgttcgta accttcctgg
     2221 aaaatcacga tcagatttgt aactcaggtt ttggtgacag aatccatttc tcttctcatc
     2281 cgggctgcta ccgggcgttg accgcgctgt tgttgctggg gccggggacg ccgctcttat
     2341 tcatgggcca ggagttcggc gcctcttcgc ccttcgtttt ttttgccgat cacaagcccg
     2401 aactagccaa gttggtctat aagggtcggc ttgagttcgt tagtcagttt accagtctcg
     2461 cgacccccga gtcccaggcg agaatccccg atcccgccgc atcgcaaact tttgaagctt
     2521 gcaagttgaa attagccgag cgtgaggagc ataaagccat ttaccagctg catcgcgatt
     2581 tattgaaact gcgccacgac gatacggtgt ttagcgctca aggggcgcac ggctttgacg
     2641 gcgcggtatt ggcgccgaaa gtttttgtcc tgcgcttttt tggccaggag aaagaagggg
     2701 atcgtctcct cgtggtcaat ctcgatgtcg actttatcta tgatccggct cccgaaccgc
     2761 tcgtggcgcc gcctgagaaa tcaaaatggc aaatcatttg gtcgagtgaa agtccggtgt
     2821 acggcggccg gggagtaacg ccgtttacag aaaattggaa aattcccgga tacgcggcga
     2881 tcgctttaac ttcagtcaat gaagcgtgat tgaaatagta aataccagtt cacgcagttt
     2941 taaagggttt gggaggattt atgcgagtgt ggccagggaa accttatccg ttaggggcga
     3001 catgggacgg caaaggggtc aactttgcat tgttttcaga ggcggccact aaggtagatc
     3061 tctgtctatt cgatggcgtc gatgccagtc aagaagcgca acgtttggag ttgaccgaac
     3121 gcaccgatca ggtgtggcat gcctatctgc cggaagcatt gccggggcag ctctacggct
     3181 atcgcgtgca cggtccttac gagccaagcc aaggccatcg cttcaatccg cataagatcg
     3241 tcctcgatcc ctatgccaaa tcgatcggcc gcgagctgcg ctgggcggac gaagttttcg
     3301 cttaccgcat gggcgacccg gaaggcgacc tgacattcga cgagcgggac agcgcgcctt
     3361 atgccccctt ggcgaccatc atcgatccgg cttttacctg gggcgatgac aagccgccgc
     3421 acacaccctg gcataagacg attatttacg agttgca
//
LOCUS       DASQKS010000591         8107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6226481, whole genome shotgun sequence.
ACCESSION   DASQKS010000591 DASQKS010000000
VERSION     DASQKS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6226481"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VE170_15060"
     CDS             complement(<1..501)
                     /locus_tag="VE170_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16815.1"
                     /translation="MHRTVLVLMTAILCCAIDIAAARDVVIVTSFPKELFETYKKTFE
                     AKHPGTTVVINSKQTNAGVTYLRETKAKPEADIFWVSAPDAFQTLDSDGLLEKYAPPK
                     EIMAKIPAKIGNFPIHDPEGKYFGFAISGYGLMWNKNYMKAHKLPPPKEWTDLTQARY
                     YNHLIIS"
     gene            complement(744..1286)
                     /gene="pyrR"
                     /locus_tag="VE170_15065"
     CDS             complement(744..1286)
                     /gene="pyrR"
                     /locus_tag="VE170_15065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HYJ16816.1"
                     /translation="MKNSRNKAKRILNAAQIKVILERFAREMAKQYGSFDQVAFIGVR
                     TRGPYIAKRLAEIVKKRHGKEIPIGEMDITLYRDDLNALLPGGTIDHTRVPFDIAGKI
                     VVLFDDVLNTGRTVRAAVDHLIDLGRPRMIHLAVMIDRGGRELPIAANYVGKRIDLKA
                     GGNVEVKLKEVDRTDEVLVQ"
     gene            complement(1273..2565)
                     /locus_tag="VE170_15070"
     CDS             complement(1273..2565)
                     /locus_tag="VE170_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HYJ16817.1"
                     /translation="MKRLLTANRPSTLPAINQRRLMRDLNAIGRIGIGNRGGVTRLVF
                     SIKELRSRQVLIHLMRQAGLKIHIDTIGNIFGRLEGSDGKAPAVLSGSHLDTVIHGGK
                     YDGPVGVIGALEAVRTIGENKIPIRSPLEVVCFVGEESSRFGFSTLGSSLVAGEVHAK
                     DLKNAVDAQGTKLADVLSSLGIAAKNLRGLARDPNSLKAYLELHIEQGPILEVIGKRI
                     GLVTSIAAPSRFKVIFTGQADHSGTTPMEMRKDALVAAALLIEFIETTCREFSSMVKG
                     RVVGTVGAIKVEPGVINAVPGRTELLVDIRAITAPAKNRVARLVKAQAQRIAHQRTLG
                     VEVLTIREEDPVPLDKRLLRLTRQICDAKQIIYEVMPSGAGHDAMQMAKITPAGMIFI
                     PSQRGISHNPLEWTAPDDIGLGTQLLMETMIRVANEKL"
     gene            2724..3713
                     /locus_tag="VE170_15075"
     CDS             2724..3713
                     /locus_tag="VE170_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16818.1"
                     /translation="MVATRLLLIAVLMLSAAPVRAEPIRIAYSGVAASGTPVWLAKEE
                     GLFAKHGLEADLVAVRSAPIQVTALVSNEVQFVRGSASSMLTAAAQGAKLKIIMSLFA
                     ERASYDFLVAPSITKPADLRGKKIGVQDFSGLLWSLTMLSLREMGLDPQRDNISIQAI
                     GDSTVIAQSLASGIIDGAALDKLQSARLQTLGVKPLLDLSKIPLPSSPFMCAEAYVLK
                     NPQIIENFIKTMIEASAFMRANKERGVTLLQKYIKVDRQLAELGYKLLLEDLTSYTFV
                     TPRGLKTAQEVIALRDPKIKNFNVEELLDQRVLTKVVKSGYVEEIERKYKLRP"
     gene            4077..4421
                     /locus_tag="VE170_15080"
     CDS             4077..4421
                     /locus_tag="VE170_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBPRA1643 family SWIM/SEC-C metal-binding motif
                     protein"
                     /protein_id="HYJ16819.1"
                     /translation="MSNRISDIGTMRSSVDADSRVNTFGRKKNAKLGSEKNPAVVRVQ
                     SELRFKEVSATFAQHGWECRISIEPDKAEDVGDLERLLHPQKPTVVAKKVGRNEPCSC
                     GSGKKFKNCCGK"
     gene            4513..4635
                     /locus_tag="VE170_15085"
     CDS             4513..4635
                     /locus_tag="VE170_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16820.1"
                     /translation="MSKGMDSKKQGKKKPQKTMMEKRAEKRAKKETKGFSIKAK"
     gene            4904..5572
                     /locus_tag="VE170_15090"
     CDS             4904..5572
                     /locus_tag="VE170_15090"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HYJ16821.1"
                     /translation="MSEVSNKVAEMLLSTESIAVYKDKPYVFVSGRISPVYIDCRKLL
                     SFAAEREYIVTALAQMAATDIGADNFDVVAGGETAGIPYAAFVAHIVKKPMIYIRKQP
                     KGYGGTKQIEGILEPGQRVLLVEDLITDGLSKLRFNIGVRGAGAKMTHCLCVFDYASD
                     RLNQHEGRESLAKNDIALHVLANWDDVLDTGTSKNYFTAEQNSQIIDFLKEPENWGRK
                     MGFV"
     gene            5690..7153
                     /locus_tag="VE170_15095"
     CDS             5690..7153
                     /locus_tag="VE170_15095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYJ16822.1"
                     /translation="MKTLIRNPLLVTMNEQNDVVDKASIVIDGNRIVFAGPAEWTPDG
                     PFDKVIDAGRMIALPGMVNAHCHSAANLVRGMMPSKPLEIWRAYYRASLRDMREDDFY
                     ASALLGGMEMLKNGATTVLDHFAGSAACRFMGAGAAIQAMRDLGLRHVAALTVTDKNY
                     EDTIPLGQTDAGLSDELKRMSASEAKSTQAWLDECEEFIATFHAPEKLTTACPGPSAV
                     QRCTDELLQGCGEIARKKNLPLHIHLAETKAQQVQGNSIYGHSLLQHLESLGIVSANL
                     SCAHSIWIEESDVELFAKRGATPVHNPASNLRIGSGLAKVKEFLAAGVRVGLGTDGAA
                     SNDNQNMFDAARLVALIHNHAGSDFNQWVTPAQALAMATRNGARAFGLEAGVIAPGKL
                     ADIVLLRRDTPAYRPMNEVVNQIVLCENGSNVDSVLVNGEVVVEGGRLAKVDEMEVLK
                     RADDARDRLAPSMQRELAAARSMEPALAEMYFRVSKG"
     gene            complement(7173..>8107)
                     /locus_tag="VE170_15100"
     CDS             complement(7173..>8107)
                     /locus_tag="VE170_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYJ16823.1"
                     /translation="SSGSGAALAAGFCFGALGSDTGGSIRTPSSHCGIVGIKPTYGRC
                     SRYGVIPLSWSLDHTGPMARSVEDCAIMLQVLAGHDAKDNASAHAPVPNFQAVMKNGI
                     KGMRVGVPRKNWFDENLGIDRQTEAVFNEALKVLESGGAVIVDIDGKAFSLARKANQT
                     LLICEAYAYHEKTYQQTPMKFGSSVRRRMLEGAFLSAADYLTAQRARAVLNEQIRANF
                     ANVDVFAVPGAARPPEAFDSMDPTEQNMRPSFTNPFNLTGLPALSVPCGFTEGNLPAG
                     LQIVAPPFEEATCFRVAYAYEQATDWHKQRPALS"
BASE COUNT         1678 a         2226 c         2442 g         1761 t
ORIGIN      
        1 gctgatgatc aaatgattgt agtagcgcgc ttgggtcaag tcggtccact ccttgggcgg
       61 cggcaacttg tgcgctttca tataattttt attccacatc aagccgtagc cgcttatggc
      121 gaagccaaaa tattttccct cgggatcgtg aatcgggaag ttgccgatct tggccgggat
      181 cttggccatg atctctttgg gcggcgcgta tttttccaac aggccatcgc tgtcgagggt
      241 ctgaaacgca tccggcgcgc tgacccaaaa tatatccgct tccggtttcg ccttggtctc
      301 gcgcaggtag gtcaccccgg cattggtctg cttgctgttg atgaccaccg tggtgccggg
      361 atgtttcgct tcaaaagtct tcttgtaggt ctcgaacagc tccttgggga acgaggtgac
      421 gatgacgacg tctctggctg cggcgatgtc aatggcgcaa cagagaattg cagtcattaa
      481 aacgagcaca gttcgatgca taataacctc ctagggtgaa aaaaaccctc gtggcggtaa
      541 gtcaccacga gggcccaatt gtgtcaagtc taattgttac aatccggtta agtgttattg
      601 gcgcagcgct tcatcgttgc cgtcaatcaa tcgaagtcga tgggcgcggt tattgtgagc
      661 cccaatgctt tagttgcggt gtttgcggtg aagacgactc aacccctgat gctcttgttg
      721 gcttcgggtc atacggcctt gcttcactgc accaacactt cgtcggtcct gtcgacctct
      781 ttcagtttga cttcgacgtt gccgccggct ttcagatcga tcctcttgcc gacgtagtta
      841 gccgcgatcg gcagttcgcg gccgccgcgg tcgatcatta cggccagatg gatcatgcgc
      901 gggcggccca aatctatcaa atgatccact gccgcgcgca cggtgcggcc ggtgttcaag
      961 acgtcgtcga acagcacaac gatcttgccg gcgatatcga agggaacgcg cgtgtggtcg
     1021 atggtgccgc cgggcagaag cgcgttcaga tcgtcgcggt agagcgtgat gtccatctcg
     1081 ccgatgggga tctccttgcc gtggcgtttc ttgacgatct cggccaagcg tttggcgatg
     1141 taagggccgc gagtgcgcac tccgatgaag gccacttgat cgaagctgcc atattgcttg
     1201 gccatctcgc gcgcgaagcg ttcgagaatg actttgattt gcgccgcgtt gaggatgcgt
     1261 tttgccttgt ttctagagtt tttcattggc gactcgaatc atcgtttcca tgagtaattg
     1321 tgtgcccaag ccaatgtcgt cgggagcggt ccattcgagc gggttgtgac tgatgccgcg
     1381 ttgactggga atgaaaatca ttcccgccgg agtgattttg gccatctgca tggcgtcgtg
     1441 gccggcgccc gacggcatga cctcatagat gatttgtttg gcatcgcaga tctgccgggt
     1501 cagccgcaag agccgcttat ccagcggcac agggtcttct tcgcgtatcg tcaggacttc
     1561 gacgcccagg gtgcgctggt gtgcgatacg ctgcgcttgc gccttaacca ggcgggcgac
     1621 gcggtttttc gccggcgcgg tgatggcgcg gatatcgacc agcagttcgg ttctacccgg
     1681 cacggcgttg atgacgccgg gttcgacctt gattgccccg acggtgccaa cgacgcggcc
     1741 tttgaccatg gaggagaact cgcggcaggt tgtttctatg aattcgatca gcagcgccgc
     1801 ggcgaccagg gcgtctttac gcatctccat cggcgttgtg ccggagtgat cggcctggcc
     1861 ggtgaagatg actttgaacc ggctcggtgc ggcgatggag gtgaccaggc cgatgcgctt
     1921 gccgatgact tccaagatcg gtccttgttc gatgtgcagt tcgagataag ccttgaggct
     1981 gttcggatcg cgggcgaggc cgcgcaggtt tttcgcggca ataccaaggc tggaaagcac
     2041 atcggcgagc ttggtcccct gggcatcgac ggcattctta agatctttcg catggacttc
     2101 gccggcaacc aggctgctgc cgagagtcga gaagccaaag cggctcgact cctcgccgac
     2161 gaagcagacg acctccaacg gcgagcggat cggaatctta ttttcgccga tggtgcgcac
     2221 cgcctcgagg gcgccgatga cgcccaccgg gccgtcatac ttgccgccat gaatgaccgt
     2281 gtcgaggtgg gagcccgaca gcaccgccgg cgctttccca tcgcttcctt ccagccgacc
     2341 gaagatgttg ccgatggtgt cgatgtggat tttcaatccg gcctggcgca tcaggtggat
     2401 caatacctgg cggctgcgta attctttgat ggaaaatacc agacgtgtca caccgccgcg
     2461 gttgccgatg ccgatgcggc cgatggcgtt caagtcgcgc atcaggcggc gctggtttat
     2521 ggcgggcagg gtggacggac ggtttgccgt gagcaggcgc ttcatgcgcc gacactgtaa
     2581 cagtttccgg ggtagctgag aattcaaatt tttctgccat tgcgattgtc gttgtcaggc
     2641 gaaccagccc catgttagaa gcggccattg gcaacgcttg attctcaaga tctctcgtaa
     2701 cctgtacagg gaggatagtc gccatggtcg ctaccagact cttgctgatc gctgttctga
     2761 tgctgtccgc cgcgccggta cgcgccgagc cgattcgcat cgcctattcc ggtgtggctg
     2821 cgtcagggac gccggtgtgg cttgctaaag aggaaggcct cttcgccaag catggactgg
     2881 aggccgacct ggtcgcggtg cgcagcgcgc cgattcaggt cacggcgctg gtctccaacg
     2941 aagtgcagtt cgtccgcggc agcgcatcga gcatgctaac cgccgccgcc cagggcgcca
     3001 agctcaagat catcatgtcg ctgttcgccg agcgggcttc ctacgatttt ctcgtcgcgc
     3061 catcgataac caagcccgcc gatttacgcg gcaagaaaat cggcgtgcag gatttcagcg
     3121 gtctgctttg gagcctcacc atgctgtcgc tgcgcgaaat gggtctcgat ccgcaacggg
     3181 acaacatcag catccaagcg atcggcgaca gcaccgtgat cgcccagtca ttggcatccg
     3241 gcatcatcga cggcgctgcg ttggataaac tgcagtcggc gcggctgcaa actctgggag
     3301 tgaagccgtt gctcgacttg agtaagatac ccctgccttc aagccctttc atgtgcgccg
     3361 aggcttatgt gctaaagaat ccgcagatca tcgagaactt catcaaaacc atgatcgaag
     3421 ccagcgcgtt catgcgcgcg aataaagagc gcggtgtcac gctgctgcaa aagtatatca
     3481 aggtcgaccg ccagctcgcc gagctgggtt acaaattgct tctcgaagac ctgacttcgt
     3541 ataccttcgt cacccccagg gggctgaaga ccgcccagga ggtcatcgct ctgcgcgatc
     3601 ccaagataaa aaatttcaac gtcgaggagc tgctcgatca gcgtgtcctg accaaggtag
     3661 tgaagagcgg ctatgtcgag gagatcgagc gaaagtataa gctccggccg taggagcgag
     3721 gagttaaatt gtgcagagcg catgtcggca ggatcagaga cgcaacgcct gatagcgctt
     3781 tgccatgaaa gctcgcccga ccattttccc gagcggcgcc tcccatgaat tcggtttttt
     3841 ctgcgatcgg cgagaaaccg cgagctagaa taaaagctac ttcccggcaa actgtcctgc
     3901 caatccgccg gactatctca gccgacgaaa actctgccgt gctgtctgag gaatctgcta
     3961 gctcgttctc gacagattcc gcccggtggt ttttctcgcg gtatccggat tcgatttgct
     4021 ccgatgttta acaatacaca tagatatatg cggcaataac tggaataata ggctctatga
     4081 gcaatcgaat cagtgatatc gggacgatgc gcagcagtgt cgacgcggat agccgggtaa
     4141 atacctttgg tcggaagaaa aacgccaagc tcggcagcga gaagaatccg gcggtggtgc
     4201 gggttcagtc ggagctgcga ttcaaggaag tcagtgcgac gttcgcccag catggttggg
     4261 aatgtcgtat cagtatcgag ccagacaagg ctgaggatgt gggcgatctg gagcgattgc
     4321 tgcatccgca gaaaccgacg gtcgtggcga agaaggtcgg gcggaacgaa ccctgctcct
     4381 gtggcagcgg caaaaaattc aaaaattgtt gtggaaagta gctgcggtga ggtggatgag
     4441 gagatgaacg ttcatcatga gggcgcgtgc ggtaggcaac gccttttaaa ccggtcataa
     4501 ggagggacgc atatgagtaa aggcatggac tcgaagaaac agggaaagaa gaagccacag
     4561 aaaactatga tggaaaagcg cgcggaaaag cgcgccaaga aagagaccaa gggtttttcc
     4621 atcaaggcaa aatgacctct gtacccgcgc tgcgggatag cgtcggctat ccctgcagct
     4681 tcggtttctt ctccatttcc gcaatgccca cctccgattc ttttttgtgc cctttgcgtt
     4741 ctttgcggcc attcttcttt tcgattgcag cttcgccgcg ctgataattt cgcggtgagt
     4801 ctgtcgcgca gcttatttta ccgccttgcc atcgggagcg ttggcaaggc caaatcgcta
     4861 tgttatgacc tgaccttaat cctgaaattt gcgaggtttt ttcatgagcg aagtgtccaa
     4921 caaggttgcc gagatgctgc tgtcgacgga gtcgatcgcg gtttataaag acaagccgta
     4981 cgtgtttgtt tccgggcgaa tttcgccggt ctacatcgac tgccgcaagc tgttgtcgtt
     5041 cgccgcggag cgggaataca tcgtcacggc gctggcgcag atggcggcaa ccgacatcgg
     5101 tgcggacaac ttcgatgtcg tcgccggcgg cgagaccgcg gggattccat acgcggcgtt
     5161 cgtcgcccat atcgtcaaga agccgatgat ctatatccgc aagcagccta aaggctacgg
     5221 cggcaccaaa cagatcgaag gcattctcga accgggccag cgcgtgctcc tggtcgaaga
     5281 tttgatcacc gacggtttga gtaagctgcg cttcaatatc ggcgtgcgcg gagcgggcgc
     5341 caagatgacc cattgtttgt gcgtgttcga ctacgcttcg gaccggctca atcagcatga
     5401 aggccgggag agtttagcga agaacgacat tgcgctccat gtgctggcaa actgggacga
     5461 tgtcttggat accggcacga gcaagaacta cttcaccgcg gaacagaatt cacagatcat
     5521 cgatttcttg aaggagccgg agaattgggg ccgtaagatg gggttcgtat aggcgcgcgt
     5581 tgcgtgccgt tcaagggttc aaggttcaaa agttcaatgt tcgggcgacg ggacagctac
     5641 ttttcagcgt ttatgaaaag ttgtctgtcc cttctttctt gagggcctta tgaaaacttt
     5701 gattcgcaat ccgctgctgg tgaccatgaa cgaacagaac gatgtcgtcg ataaagccag
     5761 catcgttatc gacggcaacc gcatcgtttt tgccggtccg gccgagtgga cgccggacgg
     5821 gcccttcgac aaagttatcg acgccggccg catgattgcg ctgcccggaa tggtgaatgc
     5881 acactgccac tcggcggcca acctggtgcg cggcatgatg ccgagcaagc cgctggagat
     5941 ttggcgcgcc tattaccggg cgtcgctgcg cgacatgcgc gaagatgact tttatgccag
     6001 cgcgctcttg ggcggcatgg agatgctcaa gaacggcgcg accaccgtgc tcgaccactt
     6061 cgccggcagc gcggcatgcc gcttcatggg cgccggcgcg gcgatccagg cgatgcgcga
     6121 tctcggcctg cgtcatgtcg cggcgctgac ggtgaccgac aaaaactatg aagataccat
     6181 tcccctcggt caaaccgacg ccggcttgag cgacgaactc aaacgcatga gcgccagcga
     6241 ggcgaaatct acccaagctt ggctcgatga atgcgaagag tttatcgcga catttcatgc
     6301 cccggaaaag ctcacgaccg catgtcccgg cccgtccgcc gtgcagcgtt gcaccgacga
     6361 gttgcttcaa ggttgcggcg aaattgcgcg caagaaaaac ttgccactgc atatccatct
     6421 ggcggaaacc aaagcacagc aagttcaggg caacagcatc tacggccatt cgcttttgca
     6481 gcatctcgaa tcgctcggca tcgtcagcgc caatctatcc tgtgcccatt cgatctggat
     6541 cgaggagagc gacgtcgaac tattcgccaa gcgcggcgcc acgccggtac ataatccggc
     6601 gagtaacctg cgtattggca gcggcctggc gaaggtaaag gagtttctcg ccgccggcgt
     6661 gagggttgga ttaggcaccg acggcgctgc ttccaacgac aatcaaaaca tgttcgacgc
     6721 agcgcgtttg gtagcgctga ttcacaacca cgccggcagc gacttcaacc aatgggtcac
     6781 ccccgcgcaa gcgcttgcca tggcgacgcg aaacggcgcc cgcgccttcg gtttggaggc
     6841 tggggtgatc gcgccgggaa agctcgccga catcgtcctg ctgcgtcgcg acacgccggc
     6901 ataccggccg atgaacgaag tcgtcaatca gattgtcttg tgcgagaacg gcagcaacgt
     6961 cgattcggtg ctcgtcaatg gcgaagtggt cgtcgaaggc ggacgactag ccaaagtcga
     7021 cgagatggaa gttttgaaac gcgccgacga cgcccgcgat cggctggccc catcgatgca
     7081 aagagaactc gccgccgcgc gcagcatgga gccggcgctg gcggaaatgt attttcgggt
     7141 gtcaaaagga tagggcgcga ttatgcgcga ccttaggata atgctggccg ttgtttatgc
     7201 cagtccgtcg cctgctcgta agcgtaagcg acacggaaac aagtagcctc ttcaaatggc
     7261 ggggcgacga tttgcagtcc agccggtaaa ttcccttcag taaatccgca cggcacggag
     7321 agcgccggca atcccgttaa attgaatgga ttggtaaaac tcggccgcat gttctgttcg
     7381 gtggggtcca tgctatcgaa cgcttccggc ggcctggcgg cgccggggac ggcgaagacg
     7441 tcgacgttgg cgaaattggc gcggatctgt tcgttgagca cggcgcgggc gcgctgggcg
     7501 gtgagataat cggcggcgct cagaaatgcg ccttcgagca ttcttcggcg caccgagctg
     7561 ccgaacttca ttggcgtttg ttggtaagtc ttttcgtggt acgcgtaagc ttcgcagatt
     7621 aagagggtct ggttggcttt gcgcgctaag gaaaaagctt tgccgtcgat atcgacgatg
     7681 acagcgccgc cgctttctag gaccttcagt gcttcattga aaaccgcttc ggtttgccgg
     7741 tcgattccca agttttcatc gaaccagttt ttgcgcggca cgccgacgcg cattcctttg
     7801 atgccgttct tcatgaccgc ttgaaaattc ggcaccggcg catgcgccga agcattgtct
     7861 ttggcgtcgt ggccggccaa gacctgcagc atgatggcgc agtcttcgac gctgcgcgcc
     7921 attggaccgg tatgatcgag ggaccagctg agcggaatga cgccgtaacg gctgcaacgg
     7981 ccgtaggttg gcttgatgcc gacgatgcca cagtgtgatg acggcgtgcg aatcgatccg
     8041 ccggtgtcgg aacccagcgc gccgaagcaa aagccggcgg cgagcgcagc gccggaaccg
     8101 ctgctcg
//
LOCUS       DASQKS010000592         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3698957, whole genome shotgun sequence.
ACCESSION   DASQKS010000592 DASQKS010000000
VERSION     DASQKS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3698957"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /gene="grxC"
                     /locus_tag="VE170_15105"
     CDS             complement(<1..205)
                     /gene="grxC"
                     /locus_tag="VE170_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HYJ16824.1"
                     /translation="MAQVTIYTTNWCPYCTGAKALLRAKEVEFEEIDVTDDPAKRAEM
                     ERLSQRRTVPQIFIDGRGIGGYDD"
     gene            297..884
                     /gene="coaE"
                     /locus_tag="VE170_15110"
     CDS             297..884
                     /gene="coaE"
                     /locus_tag="VE170_15110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYJ16825.1"
                     /translation="MGLTGGIASGKSTVAKILTELGAAIVDADILSREVVAPGHDGWK
                     EIVATFGRDVLQADQNLDRQKLRTLIFNNPDARKQLEAIIHPRVRALAEQRIRDHGEA
                     GYAVVVYEVPLLFEGNLQEWLRPVILVASNVDLQRQRLQRRDGLDATAAQKHIDAQMS
                     LEDKRKLADYVIENDGSLADLESQVRAVLAKIQAT"
     gene            complement(877..1437)
                     /gene="efp"
                     /locus_tag="VE170_15115"
     CDS             complement(877..1437)
                     /gene="efp"
                     /locus_tag="VE170_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYJ16826.1"
                     /translation="MVQATQLRPGMIIVHAGDLYRVTAVHHLTPGNKRGFMQTKMKSL
                     RTGIGTEYKFRSEDRVDQAILDNRQFQYLYAEGDMHTFMDTENYEQITLTADTIGDLL
                     NYLLPDSMVGIEFHDGKPVGINPPSTVDLAVVDTEPSMKGATASASYKHAKLETGVTV
                     LVPPFIQVGDKVRVDPTDGSYQERVK"
     gene            complement(1507..2766)
                     /locus_tag="VE170_15120"
     CDS             complement(1507..2766)
                     /locus_tag="VE170_15120"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYJ16827.1"
                     /translation="MATQEWRSDTNEMAVEQFDVAANKLGVDPNLANRLRRPDRAMIV
                     SVPTRMDDGRVHVFTGYRVQHNDVLGPFKGGIRYHPAVNLGEVSALAMWMTWKCSLVG
                     LPLGGAKGGVSCDPAELSRQELQSMTRRFTAEILNFIGPEVDIPAPDMGTNEQVMAWM
                     MDTYSQHKGHAVPEVVTGKPVAIGGTLGRREATGRGVVYTIIEAAKHLNIDLTKCTAV
                     VQGFGNVGSVAAEELTHIGVKVIGIADRTGGFVDTKGLPVDKLLQVASRNHSLEGCPY
                     GDKISNAEILELPCDILVPAAMEMQITKDNAARIRCRLLAEGANGPTTPDADAILRDK
                     GIFVIPDILANAGGVIVSYFEWVQDLQNFFWTEDEVSKKLRDILVKAFHEVLDMSQKQ
                     KVDMRMAALMIGIDRVTRAMLWRGLYA"
     gene            complement(2800..3144)
                     /locus_tag="VE170_15125"
     CDS             complement(2800..3144)
                     /locus_tag="VE170_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HYJ16828.1"
                     /translation="MSNCLFCDIVDGKIKAKLVYQDEAVVAFTDIAPKAPVHVLIIPR
                     KHIVSVLDIDANDGAVIGQIFHVAGRLARELGIAESGFRVVVNSGADAGQSVLHLHYH
                     LLGGRQMTWPPG"
     gene            complement(3237..3608)
                     /locus_tag="VE170_15130"
     CDS             complement(3237..3608)
                     /locus_tag="VE170_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16829.1"
                     /translation="MDGQKQPSGKLVGSVGELEHGKSKKFTMKRAGREFEALLVNFDG
                     THFAYVNRCPHTGITLDWVNNQFFSSDTRYLMCATHGAVFQPPTGECIWGPCVGLSLQ
                     SLPIEISDGQIYAKLPGAADE"
     gene            complement(3623..4102)
                     /locus_tag="VE170_15135"
     CDS             complement(3623..4102)
                     /locus_tag="VE170_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16830.1"
                     /translation="MALTIYLSLIFLVVYLIGLGVRRWMFIRDRNRMAVESRGGSRVV
                     ARVDEVQPGAVKKFWLICQKYRLDAILINDRGNYYAYVNRCRHMPTPLDFVRDEFFSD
                     DGRYLQCYTHGALYDFASGVCVSGPCKGESLYRLPVQVERGEVLVGCPEGDLRPMGE"
     gene            complement(4123..4446)
                     /locus_tag="VE170_15140"
     CDS             complement(4123..4446)
                     /locus_tag="VE170_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16831.1"
                     /translation="MRECVYCGESAGLFGRICSECKKLLARVDELRGNVGFGQFLDGL
                     AETGIDKEKIVKFLDADPDGKGSVRDQVTAEMTSELMKVMGIAGRQTAPEVKRIRKSV
                     NKQSD"
     gene            complement(4474..5283)
                     /locus_tag="VE170_15145"
     CDS             complement(4474..5283)
                     /locus_tag="VE170_15145"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HYJ16832.1"
                     /translation="MPVTIDFQFSQELGTAEQAARDAGAAIMALFRGKYDVHEKSKNN
                     PVTTADLEANRIIHERIAMIFPDDGWLSEEDADSEHRLGRARVWVVDPIDGTKEFIEG
                     VPQFAISIGFVVDGVAQAAVVYNPAQEEFFKAAAGHGAYLNDHAIRVTERTAIEGARL
                     LVSRSEPAKKLQVFADRCAIEPVGSIAYRLAKVAGGDGDGTLTFRTIHEWDICAGVLM
                     VEAAGGKVVDGDGHAMRFNRLPAKHRGVIAAGSALTPGLQTLWATAMNGKK"
BASE COUNT         1150 a         1674 c         1504 g         1181 t
ORIGIN      
        1 cgtcgtcata tccgccgata ccacggccgt cgataaaaat ctgcggcacc gtgcggcgct
       61 gggacaggcg ttccatttcg gcacgtttgg ccggatcatc ggtgacatcg atttcttcga
      121 actcgacttc cttagcacgc aacagcgctt tcgcgccggt gcagtagggg caccagttgg
      181 tcgtgtagat tgtcacttgc gccatgcggt ttgtctccac tccaatcttt taaatcattc
      241 tagacctttt gcttgaaagc ctctagccat tttgtaatta ctgtggcgtg aaactcgtgg
      301 gcttaactgg cggcattgcc tcaggcaaaa gtacggtggc gaaaatttta accgagcttg
      361 gcgcggcgat cgtcgacgcc gatatacttt cacgggaagt ggtcgcgccc gggcatgacg
      421 gctggaaaga gatcgtcgcc accttcggcc gcgatgtgct gcaagcggac caaaacctcg
      481 accgtcagaa gcttcgcacc ctcatcttca acaatcccga cgcgcgcaag cagctcgaag
      541 cgattatcca tccgcgcgtg cgcgccctgg ctgagcaacg tattcgcgac catggtgaag
      601 cgggttatgc cgtggtggtt tacgaagtgc cgctactttt cgaaggcaat ctacaagaat
      661 ggctgcgccc ggtgattctg gtggcgtcca atgtcgatct acagcgccag cggctgcaac
      721 gacgcgatgg cctcgacgct accgcggcgc agaaacacat cgacgcacag atgagtctgg
      781 aagataaacg aaagttagcc gactacgtca ttgaaaacga cggcagcttg gcggatctcg
      841 aatcccaagt acgtgcggtc ttagccaaga tccaagctac ttgacccgct cctgatagga
      901 tccatcggtg ggatcgacgc gcaccttgtc gccgacctgg atgaacggcg gcaccagcac
      961 cgtaactccg gtctccaact tcgcatgttt gtaggaagcg ctcgcggtgg cgcctttcat
     1021 cgacggttcg gtgtcgacca ctgccaagtc gacggtggac ggcgggttga taccgactgg
     1081 cttgccgtcg tgaaattcga tgccaaccat ggagtcgggt agcaaataat tcaaaagatc
     1141 gccgatcgta tcggcggtca gcgtgatctg ttcataattt tccgtgtcca tgaaggtatg
     1201 catgtcgcct tcggcgtaaa gatactgaaa ctggcggttg tccaggatcg cctggtcgac
     1261 ccgatcttcc gagcgaaact tgtattcagt cccgataccg gtgcgcagac tcttcatctt
     1321 ggtctgcatg aagccgcgct tgttgccggg agttaggtga tgcacggcgg tgacccgata
     1381 gagatccccc gcgtgaacga taatcatgcc cggccgcagt tgagtcgcct gaaccatttc
     1441 ttcaatacct tcctaacctg agtttcctgt tgttaacagt ttcttgtcgg cgcttcaatg
     1501 caggcgttac gcgtacagcc cgcgccagag catggctcgg gtcacccggt cgatgccgat
     1561 catgagcgcg gccatgcgca tatcgacctt ttgtttctgg ctcatgtcga gcacttcatg
     1621 aaaagctttg acgagaatat cgcgcaactt cttagagact tcgtcctcgg tccagaaaaa
     1681 attctgcagg tcttgaaccc actcgaaata agacacaatc accccaccgg cgttggcgag
     1741 aatatccgga atcacgaaaa tccctttatc acgcaggatt gcgtccgcgt ccggcgtggt
     1801 cggaccgttg gcgccttcgg ccaggagccg gcaccgaatg cgcgcggcat tgtctttggt
     1861 aatctgcatt tccatcgcgg ccggaaccag gatgtcgcac ggtagttcta agatttccgc
     1921 gttgctgatc ttgtcgccgt atgggcaacc ttcgagacta tggttcctgc tcgcgacctg
     1981 cagcaactta tccaccggca aacccttggt atcgacaaat ccgccggtgc ggtcggcgat
     2041 gccgatgact ttgacgccga tgtgggtgag ttcctcggcc gccaccgagc cgacattgcc
     2101 gaatccctgc accaccgccg tgcattttgt cagatcgata tttagatgct tggccgcctc
     2161 gatgatcgta taaaccacgc cccgtccggt cgcttcgcgc cggcctaacg tgccgccgat
     2221 ggcgaccggc ttaccggtaa ctacctcggg cacggcatgg cccttatgct gggaataggt
     2281 atccatcatc cacgccataa cctgctcgtt ggtacccata tccggcgcag gtatgtccac
     2341 ttccgggccg atgaagttca agatctccgc agtgaagcgc cgggtcatcg attgcagttc
     2401 ctggcgcgat agttcggccg gatcgcagga gacaccgcct ttggcgccgc cgaggggcaa
     2461 gccaaccaga gaacatttcc aggtcatcca catggcgagc gccgagacct cgccgagatt
     2521 caccgccgga tgatagcgga tgccgccttt gaacgggccc aggacatcgt tgtgctgcac
     2581 gcgatagcca gtaaaaacgt gaacgcggcc gtcgtccatg cgcgtcggca cgctgacgat
     2641 cattgcccgg tcaggccggc gcaaacggtt ggcaagattt ggatcgacgc cgagtttgtt
     2701 ggcggcgacg tcgaattgct caaccgccat ctcgttggtg tcgctgcgcc attcttgagt
     2761 cgccatgatt atcccaatgt ttttcggcaa ttactctttt tagcccggcg gccacgtcat
     2821 ctgccggccc ccgagtaaat ggtaatgaag atgcagcacg ctttgaccgg catcggcgcc
     2881 ggaattgacc accacgcgaa agccgctctc ggcgatgccc aactcgcgcg ctagacggcc
     2941 ggccacgtga aatatctggc cgatcacggc accgtcgttg gcgtcgatgt cgagcacgct
     3001 gacgatatgc ttgcgcggaa taatcaagac atgcacgggc gccttggggg cgatgtccgt
     3061 aaaagccacc accgcttcgt cttgatagac taatttagct ttgatcttgc cgtcgacaat
     3121 gtcgcagaac aggcaattgc tcatcgataa ctcctggacc cttgttcact tgacagggcg
     3181 cgcgggaatg tccgtgcggg gatatgcttc tttcattggc tgtcaaagcc gcttttttat
     3241 tcgtcggccg cgccgggcaa tttggcgtag atctgtccat cggagatttc gatcggcagg
     3301 ctttgcagcg acagcccaac acagggaccc cagatacatt cgccggtggg cggctgaaaa
     3361 accgcgccat gggtggcgca catgagataa cgggtgtccg agctaaaaaa ttgattgttg
     3421 acccagtcga gggtgatgcc ggtgtgcggg cagcggttga catacgcgaa gtgggtgccg
     3481 tcgaagttga ccagcagcgc ttcgaactcg cgcccggcgc gcttcatggt gaacttcttg
     3541 ctcttgccat gctccagttc gcccaccgat ccgaccagtt tacccgacgg ttgtttttgt
     3601 ccatccatag ctgtttccta tcctattctc ccatcggcct caaatcgccc tcgggacagc
     3661 ccaccaacac ctcgccgcgc tccacctgca ccggcaagcg atagagcgac tcgcccttac
     3721 agggacccga tacacagaca ccgctggcga aatcatagag ggcgccgtgg gtataacatt
     3781 gcaagtagcg gccgtcgtcg ctgaaaaatt cgtcgcggac gaaatccaaa ggcgtcggca
     3841 tgtggcggca acggttaaca taggcgtagt agttgccgcg atcattgatg agaatggcgt
     3901 cgaggcggta cttctgacag atcagccaga attttttgac ggcgcctggc tgcacctcgt
     3961 cgacccgcgc caccacccgg ctaccgccgc ggctttccac cgccatgcgg ttacggtccc
     4021 gaataaacat ccagcgccgg accccaagac caatcaaata gaccacgagg aagatcagcg
     4081 acagataaat cgtcaacgcc atggccgaga ttaccgcaag gactagtcgg actgtttatt
     4141 gacgcttttg cgaatccgct tgacctccgg cgcggtctgg cgcccggcga tacccatgac
     4201 cttcatcaat tcgctggtca tctcggcggt cacctgatcc cgcacgctac ccttgccgtc
     4261 gggatcggca tcgagaaatt tgacaatctt ctccttgtca atgccggtct cagccaagcc
     4321 gtcgagaaat tggccgaagc cgacattacc gcgcaactcg tcgacacggg cgagcagttt
     4381 tttgcactcg ctacagattc tcccaaacaa tccagcgctc tcgccgcaat agacacattc
     4441 acgcatagca attatttctt ttcgcgaata aagctatttt ttgccattca tcgccgtcgc
     4501 ccagagcgtc tgcaagcccg gcgtcagcgc cgaaccggcg gcgatcacgc cgcggtgctt
     4561 agccggcagg cgattgaagc gcatcgcatg gccgtcgccg tcgaccacct tgccgcccgc
     4621 cgcttcgacc atcaagaccc cggcgcagat atcccactcg tgaatcgtac ggaaggttag
     4681 cgtgccgtcg ccgtctccgc cggccacttt agccaagcga taggcgatgc tgcccaccgg
     4741 ctcgatggcg cagcggtcgg cgaaaacctg aagttttttc gccggctcgg agcggctcac
     4801 caaaagccgc gcgccttcga tggccgtacg ctcggtgacc cggatcgcat gatcgttcaa
     4861 gtacgcgcca tgtccggccg cagccttgaa aaactcctct tgcgccgggt tgtatacaac
     4921 cgccgcctgg gccacgccat cgacgacaaa gccgatggag atggcgaact ggggcacccc
     4981 ttcgataaac tctttcgtgc cgtcgatcgg atcgacgacc cagacccgcg cccgccccaa
     5041 acggtgctcg ctatcggcat cttcctcaga cagccaaccg tcatcgggaa agatcatggc
     5101 gatgcgctcg tggataatcc ggttggcttc gaggtctgcc gtggtcaccg gattattttt
     5161 acttttttca tgaacgtcgt acttgccgcg aaacagcgcc atgatcgcgg cgccggcatc
     5221 gcgcgccgct tgctcagcag taccgagctc ttggctaaat tgaaaatcaa tggtcacagg
     5281 catcctttca atccaatcaa accgagtaat taaacaccac aaccggcatg gcggcaattg
     5341 atctggcgaa taactagaag ccgagctcgg caatgtcgcc gatggcccgc gccccggcaa
     5401 ggtgcatttc ttcgagcgct cgggccgaat cgccggacgc cagatccacg ccacgaatcg
     5461 catccatcag cagcaccaca gcgaagcctt gcttcaaacc gtcgagtac
//
LOCUS       DASQKS010000593         8210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5370178, whole genome shotgun sequence.
ACCESSION   DASQKS010000593 DASQKS010000000
VERSION     DASQKS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5370178"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..617
                     /locus_tag="VE170_15150"
     CDS             126..617
                     /locus_tag="VE170_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYJ16833.1"
                     /translation="MKKLLIVFHSMTGGTFQMAQAAANGAQSEPDVAVNFIHAGEAGP
                     SELLQADGYIFATPENLAMMSGVMKDFFDRSYYAVLDRLVGRPYATMICAGSDGENAA
                     RQIQRIATGWRLKAIAAPIIVCTYAQTPDEILAKKLIGEFDLQRCEEIGATLAAGLAL
                     GIY"
     gene            765..1841
                     /locus_tag="VE170_15155"
     CDS             765..1841
                     /locus_tag="VE170_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ16834.1"
                     /translation="MNIVEPRGAVHWIDHFVVGTNNMSAWVDWAVNSTGITRQPIIGL
                     TTAARKRNIKITSFLWWDGGSCRIGAFLQPEFYPPAKELGKDLPRCGFYIRTEDIDKH
                     LRRLDRHKIAHTDAIRTAADGDDGTVIYFADPDGNQYELWAPVEMPAGAMDIATDEGV
                     GRISHAVFGARDLAKNAAFFAKYCGIEPEQNSKTAEGTLVLRLRAGGRLVYKLVDKVD
                     ERVSGHGTWWDMHTALTVRQEEFLPDYRRMWEGIPEEKAYKADLSLSLEEQEALPART
                     GLHRSPVGIRWKEICSRGDEFYDWDCHSFHFIGGKPLAANNSMARYEAIEQEDYLLEL
                     AQSLNKGDQPDLMKGLGKDNPFNV"
     gene            1969..2784
                     /locus_tag="VE170_15160"
     CDS             1969..2784
                     /locus_tag="VE170_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16835.1"
                     /translation="MANHIHGPLYFERMGRTGPVIAFVHPNPMDQSCWIYQMAHLSTW
                     YRCIAIDIPGYGRSPKADAGLTMDDMAQACWEAIDEVAPGEKAILGGCSVGSSIAPFM
                     YHQRPDKTAALVLCGTGYNPTKEFTKKRIDNYTAEGVGYRWRYTFEDMSAAFRVTPMA
                     HFFANMFSERNDYADAETIVHQFRALAQPYPEGHHERIACPMIILTGSEDGSHPSSFA
                     LQARVPNCELKVLPGAGHACQIEQPWLFNRFMLEFLANHGLFPGTVTPIKSIL"
     gene            complement(2959..3954)
                     /locus_tag="VE170_15165"
     CDS             complement(2959..3954)
                     /locus_tag="VE170_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16836.1"
                     /translation="MKLLPAGSLAFLLALLVADYFQAYAAAPPIKVLITTGSSSEREG
                     ALYVAQDLGYFRKYGLDVTLVQVRNGPVGMAALSSGESLLHWGSVSAANLGAIAEGAD
                     LVFVAGFINRLTGAFAANPKIKTPGELKGRSVGVNSLSGGTWIFTMLALDHWGLMPDR
                     DKIQVRALGDNSVVSQALLAGNVDAAYLSYTYAKIVQNKGFRVLADLEKLPIAYQGTG
                     LITRRSAMTSSSTTVESVIKGLLDGVAFVRNPENKLQVVKSLARGLRLKRIEDADEGY
                     QNMLGIYERKIYPSVDGVRNVIRLLGAGNEKIRRLKAEELVDDSVVKRLEKDGRF"
     gene            complement(4036..5091)
                     /locus_tag="VE170_15170"
     CDS             complement(4036..5091)
                     /locus_tag="VE170_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16837.1"
                     /translation="MRVGYLVVICFIALLTASLPVNAAESAYFQGKTVTLQVGAPAGG
                     RQDRIARTLTKYLAKYTPGSPTFLIQNKSGGQGIPAMLALSKGATDGSFMSMVISSYL
                     EAPYFGAPGATYDPRTFVYVGAPSTGKQRNVLFLHQRAGIKTLDELRKREVTLGAQGV
                     GNRSYLYARLIAELLALKVRWVLGYDTLQLYVALEQGEIQGRVNDAASMMTDRPELFA
                     KREVVALVAMTLPEELPPMPHPIFDGVPSIMQFAKTDTQRNIIRKINSTDRLGAAMAF
                     PPGTPESIRKILEEALLKVGKDAAFRKDWEDVVLEGNSFEQMFSGKEVFEDVKQYTDW
                     RPEIIAMYKRLAHEAPK"
     gene            complement(5139..5297)
                     /locus_tag="VE170_15175"
     CDS             complement(5139..5297)
                     /locus_tag="VE170_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16838.1"
                     /translation="MNPLVAISSFMTISQLGLGHGFVMATPGALERNDSTTTVVKGFD
                     DCDFIGIL"
     gene            5759..6595
                     /locus_tag="VE170_15180"
     CDS             5759..6595
                     /locus_tag="VE170_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16839.1"
                     /translation="MAFLPLIVARQNSYFDEKGLTAQLIQMGPQLQVAGVINGELDYA
                     ATFQPVVRAALQGAPLRVVTVLLAKPGHWLVSKPAIKSIAGLKGARIGIQQFGGGDHY
                     SAREILAGAGVNPDKDVTFLQIGAGPTRLAALIAQAIDASPLTEIETMRAQALNYSVL
                     KFAGDIMELPLTGISTSLAKIDKQPQQVQRVVQSLLKALQYVRQNKKGSVKILADWLK
                     LDPAQADKAYDMLFTAFSPDGRASDEGFNRLVELERKAGRLKNDVPHSRVADWSFLKE
                     TP"
     gene            6739..8076
                     /locus_tag="VE170_15185"
     CDS             6739..8076
                     /locus_tag="VE170_15185"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ16840.1"
                     /translation="MLNREDNELLTRVGPGTPMGECFRRFWLPALLSTELPRPDGPPM
                     RLRLLGEDLVAFRDSKGRAAIVEAYCPHRGTFLFWGRNEDCGLRCTYHGWKFDADGNC
                     VDMPNEPAESNFKDKVKLRSYPIREWAGLVWIYLGPNEKSPELPQFEWARLPAGHCHL
                     STWLQETNWLQGLEGEIDTSHASLLHRWLDPAAVPAYQNVAVRPDLVVQGASPRLTVN
                     ETDYGFFYGGRRPAGTDNAGQYYWRVIQWLLPTYSLIPSPQLRKAAGRCWIPIDDEHT
                     MVFSYTYSADQPLTAEELEILDAGVRFPPQLERCVFRLKNGYLIDTWRPRRNRDNDYL
                     IDREVQRSQNYTGILGINDQDRSVQETLRSAGDTWGANADRTNEKLGSSDIAIIAARN
                     RLLKLVREFQAGTDPYPAYHGDLYDIRPLDVVSSESELLQLAARYETHMRVRL"
BASE COUNT         1818 a         2467 c         2238 g         1687 t
ORIGIN      
        1 cccgtccgcc ttcccctgcc accctagtgc cccgatcgct ctaagactgt cgcgtagttc
       61 gcccaacatc cgattcatcg tagttctatg tgaactctgt gctcattgtg gttaaatccc
      121 tccgaatgaa aaaacttctc atcgtctttc actccatgac cggcggcacg ttccagatgg
      181 cccaggccgc cgccaatggg gctcagagtg agccggatgt ggcggtgaat ttcatacatg
      241 ccggcgaagc cggtccaagc gagctgctcc aagccgacgg ttacatcttc gcaacgccgg
      301 agaacctcgc gatgatgtca ggggtcatga aagacttctt cgaccgcagc tattacgccg
      361 ttctggaccg cctcgtcggc cgcccctacg cgaccatgat ttgcgccggc agcgacggtg
      421 agaacgccgc ccggcagatt cagcgcatcg ccaccggctg gcgcttaaaa gccatcgccg
      481 cgccgattat cgtctgcacc tacgcgcaga cgcccgacga gatcttggca aaaaaactga
      541 tcggcgagtt cgatctccaa cgctgcgaag aaatcggcgc cacgctagct gcgggattgg
      601 ccctgggcat ttactgaaat aacggcgcac gatcggcatt cgccttgaca cccaaccagc
      661 cgtttgctac cgctcgaaac agaacgaccg cccggctgga acgatttgaa catttttaac
      721 ggcttgaatg ttttgaaccc gctaaacact tggagggact tactatgaac atcgtcgaac
      781 ctcgcggagc ggtccattgg atcgatcact ttgtcgtcgg caccaataac atgagcgcct
      841 gggtcgactg ggcggtgaac tccaccggca tcacccggca gccgattatt ggcctcacca
      901 ccgccgcgcg caaacgaaat ataaaaatca ccagcttcct ttggtgggac ggcggctcct
      961 gccgcatcgg cgcttttctc cagcctgagt tttatccgcc ggcaaaagag ctgggcaagg
     1021 atctgccgcg ctgtggcttt tacatccgga ccgaggacat tgacaaacat ttgcgccggc
     1081 tcgaccgcca caagattgcg cacaccgatg cgatccgcac cgccgccgat ggcgacgacg
     1141 gcacggtgat ctattttgcc gaccccgacg gcaatcaata tgaactatgg gcaccggtag
     1201 aaatgcccgc gggcgcaatg gacatcgcca cggacgaagg tgtcgggcgc atcagccacg
     1261 ccgtctttgg cgcgcgcgat ttggcgaaaa atgccgcatt ctttgcaaaa tattgcggca
     1321 tcgaaccgga acaaaactcc aagaccgccg aaggcacgtt agtcctgcgt ctgcgcgccg
     1381 gcggccgcct ggtttacaag ttggtggaca aagtcgacga gcgcgtctcc ggccatggca
     1441 cttggtggga catgcacacc gcgctgacgg tgcgccagga agagtttctg cccgattacc
     1501 ggcggatgtg ggagggtatt cccgaagaaa aagcttacaa agccgatctc agtctcagcc
     1561 tcgaagaaca ggaagcgcta cccgcgcgca ccggtctgca tcgcagcccg gtgggaatcc
     1621 gctggaagga aatctgcagc cgcggcgatg aattctacga ctgggactgc cattcttttc
     1681 acttcatcgg cggcaagccg ctggcggcta acaactccat ggcgcgttac gaagcgatcg
     1741 aacaggaaga ctatctactc gaactggctc aatcgttgaa caaaggcgac cagccggact
     1801 tgatgaaagg attgggcaag gacaatcctt tcaatgtcta gcatcgcctt ttagattagt
     1861 ccgaacgatt ggaacgactg ggacgtattg aacgataagg acgcaccgtt gaaacagttc
     1921 agcgaagctc tccacgttgt ttcgaactga aagccccgaa agaattctat ggcaaatcat
     1981 attcacggac cgctttactt tgagcgaatg ggacgcaccg gaccggtgat cgccttcgtc
     2041 catcccaacc cgatggatca gtcctgctgg atttaccaaa tggcgcattt gtcgacttgg
     2101 taccgttgca tcgccatcga tatcccggga tacggccgtt cgcccaaagc cgacgccggg
     2161 ttaaccatgg acgacatggc tcaggcctgc tgggaagcga ttgacgaggt cgcacccggc
     2221 gagaaagcga ttctaggggg ttgctcggta ggctcgtcga tcgcgccgtt catgtaccat
     2281 cagcgaccgg acaaaaccgc cgccctcgtg ctctgcggca ccggctacaa tccgaccaag
     2341 gagttcacca agaagcgcat cgataactat accgccgaag gcgtcggtta tcgctggcgt
     2401 tacacctttg aagacatgag cgcggcgttc cgggtaaccc cgatggcgca tttcttcgcc
     2461 aacatgttca gcgagcgcaa tgactatgcc gatgctgaga cgatcgtgca ccagtttcgc
     2521 gccctcgccc agccttaccc cgaaggccat cacgagcgta tcgcctgccc aatgatcatc
     2581 ctaaccggca gcgaagacgg cagccatccg agctccttcg cgctccaggc ccgggttccc
     2641 aactgtgagt tgaaagtcct accgggcgcc ggccatgcct gccagatcga gcagccgtgg
     2701 ctgttcaatc gcttcatgct cgagttcctc gccaatcatg gcttatttcc cggcacggtg
     2761 acgccgatta aatcaatcct gtgagaggtc gggcgagacc cgcgattctg agagaaggtg
     2821 ccagcgctag gtgcgatact ctcggaatga cgtggaaata aacttgaatt tcgctggcgg
     2881 ctttgccgcg ctaggctttt cgtggccaac aaaactaacc ctagcgcatt atccacaccg
     2941 gcatatgaag aaacaaaatt aaaaccggcc gtccttttcc aaccgtttca ccacgctatc
     3001 gtcgaccagc tcctccgcct tgagccggcg gatcttttcg ttgccggcgc cgagcaggcg
     3061 aatgacatta cgcacgccgt cgacgctggg atagattttt ctttcgtaga tgccgagcat
     3121 attttgatag ccttcgtcgg cgtcttcgat gcgtttgagc cgcagtcctc tagccaagct
     3181 cttgacgact tgcaatttgt tttccggatt acgaacgaag gcgacgccgt cgagcaatcc
     3241 cttgatgacg ctttcgacgg tcgtcgacga cgatgtcatt gcgctgcgcc gcgtgatcag
     3301 ccctgtaccc tgataggcga tcggcagctt ctctaaatcg gcgagcacgc ggaagccttt
     3361 gttttgaacg atcttggcat aggtgtagct aagataagcg gcgtcgacat tgccggcgag
     3421 cagcgcctgc gagaccaccg aattgtcgcc cagcgcgcgc acttgaatct tgtcccgatc
     3481 cggcatgagc ccccagtgat cgagggcgag catggtaaag atccaagtgc cgccgctcaa
     3541 actattgacg ccgacacttc gccctttcag ttcccccggc gtcttgattt taggattcgc
     3601 cgcaaaagca ccggtcaagc gattgatgaa gccggcgacg aaaaccaaat ccgcgccctc
     3661 ggcgatggcg ccgagatttg ccgccgacac cgagccccag tgcagtagcg actcgccgga
     3721 gctgagcgcc gccatgccga ccgggccgtt acgcacctgc actagcgtga cgtcgagacc
     3781 atacttacgg aagtaaccta agtcctgggc gacatacagt gctccttcgc gctcactcga
     3841 tgagccggtg gtgatgagca cctttatcgg tggcgctgcc gcgtaagctt gaaaatagtc
     3901 ggcaaccagc aatgcgagta aaaaagccag agagccagcc ggtaacaact tcacatttaa
     3961 tctcctgata atgcgtagcg catactcaat ccgcggtaaa ctgaacaagc ccgcagacgg
     4021 ttgttccaat cctaatcact tcggcgcttc atgagccaat cgtttataca tagcgataat
     4081 ttccgggcgc caatcggtgt actgtttcac gtcttcgaaa acttctttgc cactaaacat
     4141 ctgctcgaac gaattgcctt ctagcaccac atcttcccag tctttgcgaa acgccgcgtc
     4201 tttgccgact ttgagcaatg cctcttcgag aatcttgcga atcgactctg gagttcccgg
     4261 tgggaatgcc atggcggcgc cgagccgatc ggtggagttg atcttgcgaa tgatatttct
     4321 ttgcgtgtcg gtcttggcga actgcataat cgacggcacc ccatcgaaga tcggatgcgg
     4381 catcggcggt agctcctcgg gcagagtcat ggcgaccagc gccaccacct cgcgcttggc
     4441 aaacaactcg ggccgatcgg tcatcatcga tgccgcgtcg ttaacccttc cctgaatttc
     4501 cccttgctcc aaggcaacat aaagctgcaa cgtgtcatag cccaagaccc agcgaacttt
     4561 cagggctaga agctcggcga ttaaccgggc gtataggtaa ctgcggttgc cgactccttg
     4621 ggcgccgagg gtgacttcgc gcttacgcag ctcgtccaga gttttgatac ccgcgcgctg
     4681 atggagaaat agcacgttgc gctgtttgcc ggtgctgggg gcgccgacgt acacgaaggt
     4741 gcgtggatca taagtggcgc ccggcgcgcc gaagtaaggt gcttccaaat atgacgagat
     4801 caccattgac atgaagctgc cgtccgtcgc tcccttactg agcgccagca tcgccggaat
     4861 gccctgcccg ccggatttat tttgaatcag gaaagttgga ctcccgggcg tgtacttcgc
     4921 caaatacttg gtcaacgtcc gcgcaatgcg atcctgcctg ccgccggccg gggcgcccac
     4981 ctgaagcgtt acggtcttgc cctggaaata agccgactcg gcggcgttta cggggaggct
     5041 cgccgtcaac aaagcgataa aacaaattac caccagataa cccaccctca tcgttcaagc
     5101 tccttttgca attgtgaaaa tgccgcctaa cgtccgtgct acaatatacc gatgaaatca
     5161 caatcgtcga atcctttaac gactgtcgtc gtcgaatcgt tccgctccag cgcgccgggc
     5221 gtcgccatga caaagccgtg cccgaggccc agttggctaa tcgtcataaa gcttgagatc
     5281 gcgacgagcg gattcattca atttccgatg atatcttccg cgtagttcca cgcataataa
     5341 aaactcaccc ggtcgcgacc tatcccgacg ctacaaagtc tgagtctact tactgtcgcc
     5401 ggcgcgaagc aagcgacaac gatcgatcca tgctcgtccg cgttccaatg gttatcggcg
     5461 gaccaatgcc gcaaagatat attttcaaca agcgccacca acggggaggg ttgtgatgtt
     5521 ctctttaccc gtgttattgc atcgacaaac ttcgaggcgt ctgtgcatag gattggatct
     5581 gggcaagttc gaaagctggc catccaagga agcgaagatt agcggaatgc ctgttcaaaa
     5641 atccagttgg tttcacccaa gccttttagc ggcagtttcg atcgccggtc tcgggttagc
     5701 tggcttacct cctttgctac cggccgaggt tgttcggatc agttattcgg cgcccggcgt
     5761 ggcatttttg ccgctcatcg tcgcgcgcca gaacagctat tttgacgaaa agggattgac
     5821 cgctcaactg attcagatgg gcccgcaact gcaagtcgcc ggggtaatca acggcgaact
     5881 cgattatgcg gcaacctttc aacccgtcgt cagggccgcg ctacaaggcg cgccgctgcg
     5941 cgtcgtgact gtcttgctgg cgaaaccagg acactggttg gtcagtaaac cggcgatcaa
     6001 atcaattgcc ggtctcaaag gcgcgcgcat cggcattcag caatttggcg gcggcgatca
     6061 ctattccgcg cgggaaatat tagccggggc cggtgtcaac ccagacaaag atgtgacctt
     6121 cctccagatc ggcgcggggc caacgcggct ggccgcgctg atagcgcagg ccatcgatgc
     6181 cagtccattg accgagatcg agactatgcg tgcgcaggct ttgaactact cggtactcaa
     6241 atttgccggc gacataatgg agctgccgct caccgggatt agcaccagcc tcgccaagat
     6301 cgacaaacag ccgcagcagg tccagcgcgt ggttcagagt ttattgaaag ctctccagta
     6361 cgtccggcag aacaaaaagg gttcagtgaa gattcttgct gattggctca aactcgatcc
     6421 tgcgcaagca gacaaagcct acgacatgct tttcactgcg ttcagtccag atggccgggc
     6481 cagcgacgag ggatttaatc gactggtgga gctggagcgc aaagccggcc ggctcaagaa
     6541 cgacgtgccg cactctcgcg tcgccgactg gagttttttg aaagaaacac cttagcaatg
     6601 ccgcgccgag gaatcagccg caaagcatgc aagcgatgaa gttcgcgtaa cctttcgccc
     6661 aggccaacga tgctcgggtc acgatgacca tgctcggccg gaagtgagcg gcgaatccga
     6721 acttatggag aggtcaccat gctcaaccgc gaagacaacg aactattaac ccgggttggt
     6781 cccggcactc cgatgggcga gtgttttcgc cgcttctggc tgcctgccct gttatcgacg
     6841 gagctgccgc ggccggatgg accgccgatg cgcttgcgcc tgctcggtga agacttggtc
     6901 gcgtttcgtg acagcaaagg cagagcagcc atcgtcgagg cctactgccc acaccgcggc
     6961 acgttcctct tctggggtcg caacgaggac tgcggcctgc gctgcaccta tcatggctgg
     7021 aagttcgacg cagacggcaa ctgtgtcgat atgcccaacg agcccgccga aagcaacttc
     7081 aaagacaagg tcaaactgcg ctcctacccg atcagggagt gggccgggct cgtctggatt
     7141 tacctggggc ccaatgaaaa gagcccggaa ctgccgcagt tcgaatgggc gcggctaccc
     7201 gccggccatt gtcatttatc gacgtggctg caagagacca actggctgca aggcttggaa
     7261 ggcgagatcg acacgtcgca tgcctcgctg ctgcaccgct ggctcgaccc agccgccgtg
     7321 ccagcgtatc agaacgtcgc ggtgcgcccc gatctcgtgg tccaaggggc gtcgcctcgt
     7381 ttaaccgtca atgagaccga ctacggtttt ttttacggcg ggcgccggcc tgccggcact
     7441 gataatgccg ggcaatatta ctggcgcgtg attcagtggc tcttgccgac ctatagcttg
     7501 attccgtcac cgcaattgcg caaagccgcc ggccgctgct ggattcccat cgacgatgaa
     7561 cacaccatgg ttttttcgta cacgtacagt gccgaccagc ctctcaccgc cgaagagttg
     7621 gagatactcg acgccggcgt gcgatttccg ccgcaactcg aacgctgcgt attccgcctc
     7681 aagaacggct atctcatcga cacctggcgc ccccggcgca atagagataa cgactattta
     7741 atcgatcgcg aagttcaacg aagccagaac tacaccggca ttctcggcat caacgaccag
     7801 gaccgcagcg tccaagaaac tctgcgcagc gccggcgaca cctggggtgc caacgccgac
     7861 cgcactaacg aaaaactcgg cagctccgac atcgccatta tagccgcgcg caatcgcctg
     7921 ctaaaactgg tccgcgaatt tcaagccggc accgatccct accccgccta tcatggcgat
     7981 ctttacgaca tccgtcccct cgatgtcgtc tcgagtgaga gcgagctatt gcagctagcg
     8041 gcgagatacg aaacccacat gcgggtgcgg ctgtaacgtt cgttcgagtt tcaagggtgc
     8101 aaaaattcaa ggtcggatcg ggcgccaccc gcgacagagg gaacgcgcaa cctggagcgc
     8161 tattgaaaac ttcgaacttc gcttcggcta actcagtgtc gagggcctgc
//
LOCUS       DASQKS010000594         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5781953, whole genome shotgun sequence.
ACCESSION   DASQKS010000594 DASQKS010000000
VERSION     DASQKS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5781953"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VE170_15190"
     CDS             complement(<1..400)
                     /locus_tag="VE170_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ16841.1"
                     /translation="MRLKDKVTIITGAAHGIGKAYARRFAEEGAHVVIADLDGVGGEA
                     TAKAILDAGGSAWARTTDVRSLPDLQGLMHETVKKFDRVDVLLNNAAIYVTQKLWKGP
                     VEDLELDEWDRVIEVNLKGVFLCSKAVIPIM"
     gene            complement(458..1318)
                     /gene="hemQ"
                     /locus_tag="VE170_15195"
     CDS             complement(458..1318)
                     /gene="hemQ"
                     /locus_tag="VE170_15195"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HYJ16842.1"
                     /translation="MEPQSPRTDIDDLPATPLSLDGSYLLHQMFRVRWPAWNELGTSQ
                     QKHVLGKAATLFRDLEVNKQAATGLFSMLGHKGDLMVVHFRKTPDDLNGAELAIAHSE
                     LNQYLEPTTSYLSVVELGLYEGSVKLYAELTAKGLKPGTAEWNREIEAELARQREAAA
                     PRLWPEIPLRRYLCFYPMNKYRGEAKNWYSEPIRERQRMMRDHGMIGRHYAGRVTQII
                     SGSIGFDDWEWGVDLFADDPLVFKKLVYEMRFDEASAVYGLFGTFYVGLRFPVSQLEV
                     LFSGKTPSFS"
     gene            complement(1341..1991)
                     /locus_tag="VE170_15200"
     CDS             complement(1341..1991)
                     /locus_tag="VE170_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HYJ16843.1"
                     /translation="MGTVEALSLAMGTAWTSGINLYATVAALGIARQMELIQLPHNLE
                     VLAHPAVIAIACIMYVIEFFADKVPYFDSGWDALHTFIRVPAGAILAARALGDMNPAL
                     ELAAMLAGGSIALAAHGAKATTRLALNASPEPFTNWTASVTEDAAVVGGIWLIFNNPI
                     LMVIFVIGFVGLGVWLTPKLFRLAKRGFQALRVRLGGSKPDRTAPSAGPPPAIINP"
     gene            complement(2092..3522)
                     /locus_tag="VE170_15205"
     CDS             complement(2092..3522)
                     /locus_tag="VE170_15205"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ16844.1"
                     /translation="MTDVTDMRGYIEVLKEMGELRELDGVDLDLEVGALTERAAEKEG
                     DALLFNNFKNYPTGFRVITNVFRTCKRTGPAMGISAQNGIDFLHEWRKKLAAYKPVPV
                     QQVEGGPVMENQMVDDEVDLYKFPTPKWHDLDGGPFIGTGCGVITKDLDTGKINVGTY
                     RVMVQDKNHVSVKMNMGKHGRLSFERARDAGKSLPIAITLGQGPAVFLAAQMPLPPDV
                     NEFEFAGYLQGAPVAVARGAFTGLPIPANAEIVLEGELPPMKDEDMPKEGPFGEWPGY
                     FTDENVGETPVMTVKRIYYRNNPILLGAPPLKPPASYLPMPLGAATLWEQLEKAGIPE
                     VKGVWGFVYGGQPGPFTVVAIKQRYAGHAKQALLVAAGARAGAYGGKFVVVVDDDIDI
                     TNPGEVIWAIATRCYVRGGIDIIKDVWASVCEPAMPPEERSPRGYTSDRVLIDACRPY
                     KWMDQFPAVNAFEKEFKDKIAGKFKL"
     gene            3798..>4095
                     /locus_tag="VE170_15210"
     CDS             3798..>4095
                     /locus_tag="VE170_15210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16845.1"
                     /translation="MQFPFFYGWIIVVIAMVAGFFASGVSNVTMAVVLKPISEDLGWS
                     RSVTAAAITLGSFGGGLLSPLCGPVADRFGPRVLLPLGGILVGLLSIGVSQSTAP"
BASE COUNT          778 a         1186 c         1219 g          912 t
ORIGIN      
        1 tcatgatcgg gataaccgcc ttgctgcaca ggaacacgcc tttcaaattg acttcaatga
       61 cgcgatccca ttcgtcgagc tccaagtcct ccaccggacc tttccagagt ttctgtgtca
      121 cataaatcgc cgcattgttg agcaacacgt caacccggtc gaattttttt acggtctcgt
      181 gcatgagccc ctggagatcg ggcagactgc gcacgtcggt tgtccgcgcc caggccgagc
      241 cgccggcgtc taggatcgct ttcgccgtcg cttcgccgcc gaccccgtcg aggtcggcga
      301 tcaccacgtg agcgccttct tcggcgaagc ggcgggcata ggctttgcca atgccatgag
      361 cggcaccggt gatgatcgtg actttgtctt tgagccgcat aggttctcca ttgccgagcc
      421 ctgttggtgg cgcagttcga aattgtccgc ggaaaaatta actgaagctt ggcgtcttgc
      481 cggaaaaaag cacttcgagc tggctgacgg gaaatcgcag gccgacgtag aaagttccga
      541 acagaccgta gaccgcgctg gcttcgtcga agcgcatttc gtagaccagt ttcttaaaaa
      601 ccaacggatc gtcggcgaat agatcgacac cccattccca gtcgtcgaag ccgatggagc
      661 cggaaatgat ctgcgtgacc cggccggcgt agtggcggcc gatcatgcca tgatcgcgca
      721 tcatgcgttg gcgttcgcgg atcggttcgc tgtaccagtt cttcgcttcg ccgcggtatt
      781 tattcatcgg ataaaaacac agataacggc gcaaaggaat ttccggccac agtcgtggcg
      841 ccgcggcttc gcgctgtcgc gccagctccg cttcgatctc gcgattccac tcggcggtgc
      901 cgggcttaag gcctttggcc gtcagttcgg catatagttt caccgatcct tcgtagaggc
      961 cgagctcgac caccgaaaga taagaggtcg tcggttcgag atactggttc aattcgctgt
     1021 gggcgatggc taattcggcg ccgttcaaat cgtcaggggt cttgcggaag tgaacgacca
     1081 tcagatcgcc cttgtgaccg agcatggaga agagcccggt ggcggcctgc ttattcactt
     1141 ccaaatctct gaacagggtc gcggcttttc ccaaaacatg tttctgctgg cttgtgccga
     1201 gctcgttcca ggccggccag cgcacgcgaa acatttgatg caacaggtag gagccgtcga
     1261 ggctcagcgg tgtcgccggc aggtcgtcga tgtcggtacg cggggattgg ggttccattc
     1321 ggcttgtcgt ttcctgtggt tcagggatta ataattgccg gcggcggacc tgcgctaggc
     1381 gcggtccgat cgggtttgct gccgcccagg cgaacgcgca gggcttggaa gccgcgtttg
     1441 gccaaacgaa ataattttgg cgtaagccag actcccagcc cgacgaagcc tataacgaaa
     1501 atgaccatga ggatgggatt attgaaaatc aaccagatgc cgcctaccac ggcggcatct
     1561 tcggtaacac tggcagtcca attcgtgaac ggttccgggc tggcattgag cgccagacgt
     1621 gtggtcgctt tggcgccatg ggcggcgagt gcgatcgaac cgccggcgag catggcggcc
     1681 aattccagcg ccggattcat atcgccgagg gcacgcgcgg ccaagatcgc cccggcgggc
     1741 acgcggataa aagtgtggag ggcgtcccaa ccgctgtcaa agtagggcac cttatcggcg
     1801 aaaaactcga tgacgtacat gatgcaggcg atggcgatca ccgctggatg ggcgaggact
     1861 tcgagattgt gcggcagctg aatcagttcc atctgccggg cgatacccaa ggccgccacc
     1921 gtggcgtaaa gatttatgcc cgaggtccag gctgtcccca ttgccaagct taatgcttcg
     1981 acggtgccca tgtgcgcact cctttttcgc cgcgcggccg ccggatactg acctgttgga
     2041 cgttgattcg agcgatatgt tgcgtctccc cgcgggccaa cgggaaatct cttacagttt
     2101 gaacttgccg gcgatcttgt ccttgaactc tttctcgaag gcattgactg cgggaaactg
     2161 atccatccat ttgtacggcc ggcaggcgtc gatgagcact cggtccgaag tgtaaccgcg
     2221 tggcgaccgc tcttcgggcg gcatcgccgg ctcgcacacc gaggcccaaa cgtctttgat
     2281 gatgtcgatg ccgccgcgca cgtagcagcg cgtggcgatg gcccagatca cctctcctgg
     2341 attggtgatg tcgatgtcgt cgtcgacgac gacgacaaac ttgccgccat aagcgccggc
     2401 gcgggcgccg gcggcgacca gtaacgcttg cttggcgtgg ccggcgtagc gctgtttgat
     2461 cgcgaccacg gtgaacggcc cgggctgtcc gccgtaaaca aaaccccaca cccctttaac
     2521 ttccggaatg ccggcctttt ccaattgctc ccacaaagtc gccgcgccca gcggcattgg
     2581 cagataactc gccgggggct tgagcggcgg cgcgccgagc aggataggat tgttgcgata
     2641 gtaaattcgt ttcaccgtca tcaccggcgt ctctccgaca ttttcatcgg taaaatatcc
     2701 cggccattcg ccgaagggac cttccttcgg catgtcttca tctttcatcg gcggcagttc
     2761 gccttcgagg acgatttcgg cgttagccgg aatcggcagt cctgtgaatg cgccgcgggc
     2821 gacggcgacc ggcgcgcctt gcagataacc ggcgaattca aactcgttga catcgggcgg
     2881 cagtggcatc tgggcggcga gaaatactgc cgggccttgg cccagggtga tggcgatggg
     2941 caacgacttg ccggcatcgc gagcgcgctc gaaagagagc cggccatgct tgcccatgtt
     3001 catttttacc gagacgtgat ttttgtcctg caccatgacc cggtaggtgc cgacattaat
     3061 tttacccgtg tccaagtctt tggtgatgac gccgcagcca gtgccgataa acggtccgcc
     3121 gtcgaggtca tgccacttgg gtgtgggaaa cttatagaga tcgacctcat cgtcgaccat
     3181 ttgattttcc atcactgggc cgccttcgac ctgctgcacc ggcacgggct tataggccgc
     3241 gagcttcttg cgccactcgt gaagaaaatc gatgccgttt tgcgcgctga tacccatggc
     3301 cggaccggtg cgcttgcaag tccgaaagac attggtgatg acgcgaaacc cagtcggata
     3361 atttttaaaa ttattgaaca gcagcgcgtc gccttctttt tccgccgccc gttccgtgag
     3421 cgcgcctacc tccaagtcca aatccacgcc gtcgagctcg cgcagctcgc ccatctcttt
     3481 gagcacttcg atgtagcccc gcatgtcggt aacgtcagtc atcggttcct cctaaattga
     3541 cgcaatacta tggcggcggg ggaggtgagt caatcgaagg tggctctttc acctgcttca
     3601 tagcccggtg tcgcactcga atgttcagac aatgcgcgct cgtatctggc gctcggtcgg
     3661 attggcttaa tgcagttcga gcggactccc ggcagagatg cagaggatcg cgctttggtc
     3721 ccgcgtgacc ttgttctgcg tcatgtcccg gacaaattat ttgagaattt cttgcgcaac
     3781 tgtagtaagg ctactaagtg cagtttcctt tcttctacgg ctggatcatc gtcgtcatcg
     3841 ccatggtcgc agggtttttc gcatccggcg tgagcaacgt taccatggcg gtggtgttga
     3901 agccgatcag cgaagatctc ggctggagcc gcagcgtaac cgcagcggcg attaccttgg
     3961 gatcttttgg cggcggcctg ctgtcgccct tgtgcggacc ggtggcggat cgatttgggc
     4021 cgcgggtgtt gttgccgctc ggtggtatcc tggtcggcct gttgtccatt ggcgtgagtc
     4081 agagcacggc gccgt
//
LOCUS       DASQKS010000595         6087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1584503, whole genome shotgun sequence.
ACCESSION   DASQKS010000595 DASQKS010000000
VERSION     DASQKS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584503"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VE170_15215"
     CDS             <1..1179
                     /locus_tag="VE170_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ16846.1"
                     /translation="RGFQHDTRMPSLRLSDDEAFSITNYLMSLGGRPEPVAGIQEKLA
                     DANNIKRGETLVRKWGCSGCHDIKGMEKESRIGAELTTFGSKTVEELSFGNRTDVKHT
                     WDDWTYYKIKTPRGYATERVEQLMPQFDLEDEDIKALRMLLGGFRERKVGHRYVADQG
                     SRVQQVVEGRRLMQQYNCVGCHEIEKRGGFVRKYYQENVTLAPPPLNGEGEKVQSPWL
                     FSFIKSPTTLRPWLDIRMPTFGLSDEHTTQLVNYFNGLSKVEQPFHYIDDRQIPPAHI
                     EAGKILTSADYFNCFSCHVQGGKNPEGPKDGWAPDLAMARQRLNPNWIIKWIKDPQKI
                     QPGTKMPSFYPGGPDNILGGKEDLQIEALRDYLISLGRGGATAAAPVAPQVAKTKPAK
                     "
     gene            1250..1963
                     /locus_tag="VE170_15220"
     CDS             1250..1963
                     /locus_tag="VE170_15220"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYJ16847.1"
                     /translation="MNKFGFLMLTGALVTAPVTVMAQYTGGDVKDGGSISGTVKFKGT
                     APAPKKLDVGKDKEVCDKSPKMDPTLIVKDGNLANAVITITDIKSGKKMDAKKVTLDQ
                     AGCEYKPHVLAFQAGSTVEILNPDGILHNVHSYSKVNSPFNQAQPKFKKTLEVKIDKP
                     EAVEVKCDVHGWMHGWLVATANPYFAVTDGSGSFKLTDVPAGSYTVEVWHEKLGKSTQ
                     KVAVKAKEDAKVSFEVAGK"
     gene            complement(2033..2722)
                     /locus_tag="VE170_15225"
     CDS             complement(2033..2722)
                     /locus_tag="VE170_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ16848.1"
                     /translation="MTTLDQLRKDIISASRILSHLKLVEGFGHVSARIPDSDRFLITP
                     RISLAMVSEEQLLTLNMDGEIVEGNHPAPFEAWLHTAILKTKPRVNAITRIHARVANI
                     FSVTDRRLEPVHNHGSFFAGGVPIFTPPDLISTEKLGNDVSETLANRPAVLLRGNGQV
                     TVGRTIPEAVMMAIYLEEAAEVLYGALQIGTPIPLTVDESKQRQIEALPPVDMERAWN
                     FFKNKVERASR"
     gene            2795..3064
                     /locus_tag="VE170_15230"
     CDS             2795..3064
                     /locus_tag="VE170_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16849.1"
                     /translation="MKIKVESSADSKGQESPTLFMLGARTIKVEACVDRWYGEQACYF
                     RVLGSDENSYILKGPLEDGSWEMVSFTHKDSRGTELEYEGKRELQ"
     gene            3152..4495
                     /locus_tag="VE170_15235"
     CDS             3152..4495
                     /locus_tag="VE170_15235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ16850.1"
                     /translation="MSNQHLVAVVGAGPAGLYGARKLTEAGHRVVLINRDIKPGGLAE
                     YGIFVDKEKMKTGLRKQFKRILADPKVFYLGHVMVGQNQALSLNDLRAMGFSAVVVTA
                     GAQGTKKLGIEGEEAVGVYHAKDVVYHFNSLPPFAERQFEIGRRVAIIGMGNVMVDIA
                     NWLLNLKKIDQVIVVARRGPLEKAYDDSEFEYIDMYLDNADMKQEIERISGSLAAVGQ
                     DVGAIMKKMVRQGEPLEGRRLTFRYLCSPNKVVANAQGRVAVLEAEENEMVLQDGKTV
                     AKGTGKTRRIEVDCVIYAIGDQVDASLGLPFSRGAFVTNPEKLPGDPNPAAYQPYDPE
                     KKQILDGIFVAGWSRNASVGLVGVAKQDAERGMKVVNDYLAAKPGLSAGDMEAKIEGL
                     IDALKQKGVTVVTKPDVELLEGAEQEAAKARKTWDFKYTSDEKMLEVIAARRAGK"
     gene            4844..>6087
                     /locus_tag="VE170_15240"
     CDS             4844..>6087
                     /locus_tag="VE170_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HYJ16851.1"
                     /translation="MLAVVESKAAYRLPGDGLQQAKDYAEILGVKFAYSTNGHGIVEF
                     DFITGQERQLASFPKPNDLWQRFRASEKIDDDETANKLLTPANSAIGKGQRYYQEIAI
                     NRVVQSVLQDKKRILLTMATGTGKTAVAFQICWKLWNMRWNRAGEHRRPKILYLADRN
                     ILIDDPKDKMFTTFGDARWKIENGEINKGREIYFAIYQAIAKDERRPGLYKEYGKDFF
                     DLIIIDECHRGSAKDQGNWREILRYFQPAFQLGMTATPLRKENRDTYRYFGNPVYTYS
                     LRQGIEDGFLAPYRVHRIVSEWDAAGWRPSQGDLDHYGREIPDSEYQTVDFERAVALK
                     ARTEAIARNITEFLNKTDRFAKTIVFCVDQEHADEMRRALNNLNDDLVKQYPDYVCRI
                     TSDEGSTGKGYLSKFQELETISP"
BASE COUNT         1471 a         1578 c         1760 g         1278 t
ORIGIN      
        1 cgcggcttcc agcatgacac gcgtatgccg agcttgcgcc tgtccgacga cgaggctttt
       61 tccatcacca actatttgat gagcttgggc ggcaggccgg agccggttgc cggcattcag
      121 gaaaagctcg ccgatgccaa caatatcaag cgcggcgaga cactggtgcg taaatggggt
      181 tgttccggct gccatgatat caagggcatg gagaaggaat cgcgcatcgg ggccgagctg
      241 actacctttg gttccaagac ggttgaagag ctatctttcg gcaatcgcac cgacgtcaag
      301 catacctggg acgattggac atattataag attaagacgc cgcggggtta tgcaaccgag
      361 cgcgtcgagc agctgatgcc gcaatttgat ctagaggacg aggacatcaa agctctccgg
      421 atgttgttgg gcggctttcg cgagcgtaag gtcggtcatc gttacgtggc cgatcaaggg
      481 tcgcgcgtcc aacaggtggt cgaaggccgg cgcttgatgc agcagtacaa ctgcgtcggt
      541 tgtcacgaga tcgaaaaacg cggcggtttc gtgcgcaagt attatcagga gaatgtgacg
      601 ttggcgccgc cgccattgaa cggcgagggc gagaaggtgc aatcgccgtg gcttttcagc
      661 ttcataaaat cacctactac cttgcgcccc tggttggata ttagaatgcc gaccttcggc
      721 ttgtccgatg agcataccac gcaactggtg aactacttta acggtttgtc gaaggtcgag
      781 cagccctttc attacatcga tgatcgccag attccgccag cgcacataga agctgggaaa
      841 attttgactt ccgccgatta tttcaactgc tttagttgcc atgtccaggg tggtaagaat
      901 cctgaggggc cgaaagacgg ttgggctcct gatctagcta tggcgcggca gcggttgaat
      961 cccaactgga tcatcaagtg gatcaaagat ccgcagaaaa ttcagccggg gacaaaaatg
     1021 ccgtcgttct atccgggcgg gccggataac atcctgggcg gcaaagaaga cttgcagatc
     1081 gaggctctgc gcgactactt gatatcgctg ggtcggggcg gggcgacagc ggcggcccct
     1141 gtggcgccgc aggtggctaa aactaaaccg gcaaagtagc gattaataga tgaatattgc
     1201 tgtggcctgg tcgtccaacc gatgaccagt tcggttgaag gaggaattta tgaataagtt
     1261 tggttttctt atgttgactg gcgctttggt aaccgcgccg gtgacggtaa tggcgcagta
     1321 tacgggtggg gatgtgaaag atggcggatc aatttccggt accgtcaaat tcaaagggac
     1381 agcgccagcg cccaaaaagc tcgacgtcgg caaagataaa gaagtttgcg acaaatcgcc
     1441 aaagatggat ccgactttga tcgtgaaaga cggcaacctg gccaatgcag taataacgat
     1501 cacggatatc aagagcggca agaagatgga tgccaaaaaa gtaacgctcg accaagctgg
     1561 ttgcgagtat aaaccgcatg tactagcttt tcaggctggg tcaactgtgg aaatcctcaa
     1621 tcctgacggt atccttcata acgttcattc ctatagcaag gttaacagcc cgttcaatca
     1681 ggcccagccc aagttcaaaa agaccttgga agtgaagatc gataaaccgg aagcggtgga
     1741 agtgaagtgc gatgtccacg gctggatgca tggttggctc gtggccaccg ccaaccccta
     1801 tttcgcggtt accgacggat cgggcagttt caagctgact gatgtgcccg ccggtagcta
     1861 caccgtcgag gtgtggcatg aaaagctcgg caagagcacc cagaaggttg cggtcaaggc
     1921 gaaggaagac gcgaaggtaa gcttcgaagt cgccggcaag taattttttc gaatcaatcg
     1981 ataaaaagcg cggcatcttc tgggtgccgc gctttttgcg tttttaagcc acttatctgc
     2041 tcgcgcgctc caccttattc ttgaagaaat tccaggccct ctccatgtcc accggcggca
     2101 acgcttcgat ctgccgctgt ttcgattcgt cgacggtcaa agggatcggc gtgccgatct
     2161 ggagcgcgcc gtaaagcacc tccgccgcct cttccaaata gatcgccatc attaccgctt
     2221 ccggaatcgt tctgcccacc gtcacctggc cgttgccgcg cagtagcaca gccggacggt
     2281 tagccaaggt ttcgctgaca tcgttgccaa gtttctccgt cgagatcaga tcgggagggg
     2341 tgaatatcgg cacgccgcca gcaaagaaac tgccatgatt gtgtaccggc tcgagccggc
     2401 ggtcggtgac tgaaaaaata tttgccacgc gcgcgtggat gcgggtgatc gcgttaaccc
     2461 gcggcttggt tttgaggatc gccgtgtgca accaagcttc aaagggcgct gggtggttgc
     2521 cttcgacgat ctccccgtcc atgttcaggg ttagcaattg ctcctcgctg accatagcca
     2581 ggctgatacg cggggtgatc aagaagcggt cgctgtcggg gatgcgcgcg ctgacatgac
     2641 cgaagccttc gacgagtttc aggtgcgaaa gaatccggct ggcgctaata atatcttttc
     2701 tcagctgatc gagggtggtc atgggactcc tttaaagtcg gaaaattttg ggctatatta
     2761 cgtcgttccg attgctgagt taagcgagtc caatatgaaa attaaagtcg agtcatctgc
     2821 cgattccaaa ggtcaggaaa gtcctacgct gttcatgctc ggagcgcgga ctatcaaagt
     2881 cgaagcctgc gtcgatcgct ggtacggcga gcaagcttgc tactttcggg ttctcggcag
     2941 cgacgagaat tcctacattc tcaaaggacc gcttgaagat ggcagttggg aaatggtttc
     3001 ctttacccat aaggactccc gcggtaccga gttggaatac gaaggcaaaa gggaactcca
     3061 gtaagttagg gcgccttttg cttctctagt ttgttctgca attcatctct caacggcgag
     3121 agaaatacaa ttccggttat gaggtagggc aatgtctaat caacatttgg tggcggtggt
     3181 cggtgcgggg cccgcgggac tttacggagc gcgcaaattg accgaagcgg gccatcgagt
     3241 ggtcttgatc aatcgcgaca tcaaaccggg ggggttggcg gagtacggca tctttgtcga
     3301 caaggaaaag atgaaaaccg gcttgcgcaa gcagttcaaa aggattctcg ccgacccaaa
     3361 agtattttac ctgggccatg taatggtagg acaaaaccag gcgctgtcgc tcaacgatct
     3421 gcgggccatg ggcttcagcg cggtggtggt gaccgcggga gcacaaggga ccaagaagct
     3481 gggcatcgag ggtgaggagg ccgtcggcgt ttaccatgcc aaggatgtcg tctatcactt
     3541 caattcgttg ccgcccttcg ccgagcgcca gttcgaaatc ggccggcggg tggcgatcat
     3601 cggcatgggc aacgtcatgg tcgacatcgc caactggctg ctcaatctga aaaagatcga
     3661 tcaagtgatt gtcgtcgcgc gtcgcgggcc gctggaaaaa gcctatgacg acagcgagtt
     3721 cgagtatatc gatatgtatc tcgacaatgc cgacatgaaa caggaaatcg agcgcatcag
     3781 cgggtcgcta gccgccgtcg ggcaagatgt cggcgcgatc atgaagaaaa tggtgcgcca
     3841 gggcgagccc ctggaagggc gccggctcac cttccgctat ctctgttcgc cgaacaaagt
     3901 cgtcgccaat gcccaaggcc gggtcgcggt cctggaagcg gaagaaaacg agatggtgct
     3961 gcaagacggc aaaaccgtcg ccaaggggac cgggaagacc cgacgcatcg aagtcgactg
     4021 cgtgatttac gccatcggcg atcaagtcga tgcatccctc ggtctgccgt tttcccgcgg
     4081 tgccttcgtc accaatccgg aaaaacttcc cggagatcct aatccggccg cctatcagcc
     4141 ctatgatcct gaaaagaagc agatcctcga cggcatcttc gtcgccggct ggtcgcgcaa
     4201 tgccagcgtc ggtctcgtcg gcgtcgccaa acaagacgcc gagcgcggca tgaaggtggt
     4261 caacgattat ctcgccgcca aacccggtct ttccgccggc gatatggaag cgaaaatcga
     4321 gggtttgatc gatgccttga agcaaaaggg cgtgacggtg gtgaccaaac cggacgtcga
     4381 actcctcgaa ggcgccgaac aagaagccgc caaagcgcgc aagacctggg atttcaaata
     4441 caccagcgat gaaaagatgt tggaagtgat cgcggcgcga agagcaggta agtagaagca
     4501 ggatagaaat aaaatcggtt caacgatggc cggctgggca tatacgcccg gctggctttt
     4561 tttgtgcctg aagggtccaa ggccgtcatc cttgctctaa cgtcgcaaga tcgcatagta
     4621 tcgacggaaa ttccgttccg ctctcttagc gacttgccgt gatcaccaag ccgacacctg
     4681 tagaaaatac gtcctgccga aactctatgc cgctggctgg aacgacgatc agatcagcga
     4741 gcaaaagagt tttacggacg gacgcattct cgtggttggg accaaggcgc gccgccggcc
     4801 ttcgaaacga gccgactacc ttttgcgtta cgccagggat ttcatgctgg cggtcgtcga
     4861 atcaaaggcg gcctacaggc tccccggcga cggtctgcaa caagccaaag attatgccga
     4921 aatccttggc gttaagttcg cttactccac caacgggcat ggcatcgtcg agtttgattt
     4981 catcacaggt caggagcgcc agctcgcttc gtttccaaag ccaaacgact tgtggcaacg
     5041 ctttcgcgcc agcgagaaga ttgacgacga tgaaaccgcc aacaaactct taacgccggc
     5101 caattcagcg atcggcaaag gccagcgcta ctaccaagaa attgccataa accgggttgt
     5161 ccaatcggtg ctgcaagata agaaacgaat cctgctcacc atggccacgg gtacaggcaa
     5221 gacggcggtg gcatttcaga tctgctggaa gctctggaac atgcgctgga accgagctgg
     5281 agagcaccgg cgccctaaga ttctatatct ggcggaccga aatattctaa tcgacgatcc
     5341 caaggacaag atgtttacga ccttcggcga cgcccgctgg aagattgaaa acggcgagat
     5401 caacaagggg cgcgagattt atttcgcgat ttatcaagcc attgccaaag atgagcggcg
     5461 ccctgggttg tataaagaat atggaaaaga tttcttcgac ttgatcatca tcgacgaatg
     5521 tcaccgcggc agcgccaaag atcagggcaa ttggcgcgag atattgcgtt acttccagcc
     5581 ggcttttcag ttgggtatga cggcgacgcc gttacggaaa gaaaatcgcg atacttatcg
     5641 ttatttcggc aaccccgtct acacttatag tttgcgccaa ggcattgagg atggttttct
     5701 cgcgccctat cgagtgcacc gtatcgtgag cgaatgggac gcggcgggct ggcggccgag
     5761 ccaaggcgac ctcgatcact acgggcgcga gattcccgac agcgaatacc agaccgtcga
     5821 tttcgagcgc gccgtcgcgc tcaaggcgcg caccgaagcg atcgcgcgca atatcaccga
     5881 atttctcaac aagaccgatc gcttcgccaa gacgattgtt ttctgcgtcg accaggaaca
     5941 tgccgacgag atgcgtaggg cgctcaataa tctaaacgac gatttggtga aacagtatcc
     6001 cgattatgtt tgccggatca cgtctgatga gggcagcacg ggaaaaggct atctgagcaa
     6061 gtttcaggag ctcgaaacga tcagccc
//
LOCUS       DASQKS010000596         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4945442, whole genome shotgun sequence.
ACCESSION   DASQKS010000596 DASQKS010000000
VERSION     DASQKS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4945442"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VE170_15245"
     CDS             complement(<1..212)
                     /locus_tag="VE170_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16852.1"
                     /translation="MLQAEKVTPANETYARASTPTGWGIRLAYFFLDSTPLGWLVRQV
                     AAIQASVHAKLITGFFIVTFLVVAMGG"
     gene            351..770
                     /locus_tag="VE170_15250"
     CDS             351..770
                     /locus_tag="VE170_15250"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ16853.1"
                     /translation="MIKITEEMQQRVDKAFEEKKYCVWATTSDDGFPDMSFRGSTFVF
                     DDEHLAFWDRSFGASTTNLESNPHVCMLFYDKENRLGWRFYGTATIHKDGDLRQQIMR
                     RTVKGELDKDPERKGYGVLVRVDKIRGYSGFNVVQER"
     gene            844..1788
                     /locus_tag="VE170_15255"
     CDS             844..1788
                     /locus_tag="VE170_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16854.1"
                     /translation="MVAIASCFCVGFHSHAAERIRIAVSNPNMPNLTAQMALKRGFFR
                     DENLDAEIIRMNPNVAITALATGDVDYCQLFGAVIGGAVSGLPVRIVAGFLDNWPMTL
                     IAQPEIKTLKDLKGKTLGISSFGATPDVAARMMIKQAGIDSDKDIKILALGSDAARMT
                     ALMQRIVDVVVMSPPADTQLEKQGYKILGRAYELFNFPYLGLGTHLRKIKERPQEIRR
                     AIKATIRANRFIRDNRDESARTLIAWGKVEREFAYASYDALRVLFNSDGAVPEDGLKL
                     VIDQARRSAKVTREVAANDVVDLTFLREAQAELGIKGR"
     gene            2003..2935
                     /locus_tag="VE170_15260"
     CDS             2003..2935
                     /locus_tag="VE170_15260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16855.1"
                     /translation="MIIDWHAHVYPPELAKERRWGGASPLTIENLLEAHEKAGIERCV
                     VSNTIHYLKDKSADESLAFLRRWNEYGAEIQQKYKDRIIVFTSTLPCGGAPFNKELER
                     AVTEYGLHGVLINSSHQHAYPDDDEAMPFFELVSSLGIPMMMHAPSVGYGEERMRDYR
                     LASSVGRPFDECLSIARLIVRGIFERHSKLKFVGCHLGGGICDVIGRMDYAYELGDQA
                     SGLGSYEPMLITKKPSEYLKTIHMDTVAYHPPAVMCAYQTVGARQLLFGSDAPPLLPL
                     LPRAKQIIEELPIGDAEREAIFSGNALRLLGRAG"
     gene            complement(3140..3277)
                     /locus_tag="VE170_15265"
     CDS             complement(3140..3277)
                     /locus_tag="VE170_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16856.1"
                     /translation="MIDRHGIEAVDADDDGVGVVWLRIALEPAVILSIVHRGLSFDLE
                     H"
     gene            3313..3588
                     /locus_tag="VE170_15270"
     CDS             3313..3588
                     /locus_tag="VE170_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HYJ16857.1"
                     /translation="MAGLSYDDLIEPTIVKQESKSVAYCRCTRSKNLPFCDGSHEGSN
                     IQPAIIELASPETIAICRCWRSKDHPYCDGTHGRLVKPKERPPRAHG"
     gene            3675..4055
                     /locus_tag="VE170_15275"
     CDS             3675..4055
                     /locus_tag="VE170_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ16858.1"
                     /translation="MVEMKNLVKEADTHPGVGHLSLWRTDLAYYWVINCEAGLQDDMH
                     YHDNDDHIFMCVEGECTVRTPHKAFVLKQFDTVLLQSGESYQLCNTGTGRMIPDDSDG
                     RRDRYDRQRQGHETHGESQQNVGK"
BASE COUNT          977 a         1188 c         1114 g          799 t
ORIGIN      
        1 ccgcccatgg cgacaaccaa gaaggtgact atgaaaaacc cggtgatcag tttggcatgc
       61 accgacgcct ggatcgccgc cacctggcga accagccaac cgagcggtgt actgtcgaga
      121 aaaaagtacg cgagtctgat cccccagcca gtcggcgtcg acgctctggc gtaggtctcg
      181 ttagccggag tgactttttc cgcttggagc atgcgttagc tctaaaattt gtcgcagcgg
      241 acgcttacaa agcctggcaa taaccgccga aacactagcc atcgctggga caatcgcttg
      301 acagccgccg tgcttcccca taggaaatat tgcaacgggg acgaaacaac atgatcaaga
      361 tcaccgagga aatgcaacag cgagtcgaca aggctttcga ggaaaaaaaa tattgcgtct
      421 gggcgaccac ctcggacgac ggatttcccg acatgagctt tcgcggcagc acgtttgttt
      481 tcgacgatga gcatctcgcc ttctgggacc gctccttcgg tgccagcacg accaatctgg
      541 aaagcaaccc gcatgtctgc atgctcttct atgacaaaga aaaccgcctg ggctggcgct
      601 tctacggcac ggcgacgatc cacaaggacg gcgatctccg ccagcagatc atgcgccgca
      661 cggtcaaagg cgaactcgac aaagatcccg agcgcaaagg ctacggcgtc ctcgtgcgcg
      721 tcgataagat tcgcggctac agcggcttca acgtggttca ggagcgctag ccgacggcta
      781 ggttcctggt ttcgaatgac aaatctcaga tttcaaagtc ggagctcgtt tcggttaatc
      841 ttcatggtag cgattgcgag ctgcttctgc gtcggctttc atagtcacgc cgctgagcgt
      901 attcgcatcg cggtgtcgaa tcccaacatg cccaatctca ccgcgcaaat ggcactcaag
      961 cgcggtttct tcagggatga aaatctcgat gccgagatca tccgcatgaa tcccaacgtc
     1021 gcgatcacgg cgctggccac cggcgacgtg gactactgcc aactcttcgg cgccgtgatt
     1081 ggcggcgcag tgtccggcct gccggtgcgg atcgtcgcgg ggtttctcga caactggccg
     1141 atgaccctga tcgcccaacc ggagatcaaa acgctcaaag atctcaaagg caaaaccctc
     1201 ggcatcagca gcttcggcgc cactcccgat gtcgccgcgc gcatgatgat caaacaggcc
     1261 ggcatcgata gcgacaagga catcaaaatc ctcgccctcg gctccgacgc cgcgcgcatg
     1321 accgcgctca tgcagcgcat cgtcgatgtc gtcgtcatgt cgccgcccgc cgacacccag
     1381 ctggagaaac aaggctataa aattttgggg cgcgcctacg agctattcaa tttcccctat
     1441 ctcggtctcg gcacccatct gcggaaaatt aaagagaggc cgcaggaaat acgccgcgcc
     1501 atcaaagcaa cgataagagc caatcgcttc attcgcgaca accgcgacga gtccgctcgc
     1561 acgctgatcg cctggggtaa agtcgaacgc gaattcgcct acgcctccta cgacgcgttg
     1621 cgagtgctgt tcaacagcga cggcgccgtg cccgaagacg gtttgaagct ggtcatcgac
     1681 caagcgcggc ggagcgccaa agttacccgt gaagtcgcag ccaacgatgt ggtggattta
     1741 actttcctgc gcgaagctca agcggagctt gggatcaagg gacgatagtg tccggttcac
     1801 ttcctcgacg aattcgtcgc aactgttatt tctcgaaagc cgaggaaacc gtcttctata
     1861 gatgacagca cttgaatttg gcgtattcac agacagtccc acagtgtgga aaaagttcag
     1921 aggccgtagc tctttgaggt tcgaaccaac gatttgaacg tcttgaacga ttgtacgatt
     1981 ggaccagatt caggagtacc caatgattat cgactggcac gcccatgtct atccgcccga
     2041 gttggccaaa gaacgccgtt ggggcggcgc gtcgccgctg accattgaaa acctgctcga
     2101 agcccatgaa aaggccggca tcgagcgttg cgtcgtcagc aatacgatcc attatctgaa
     2161 agacaaaagc gccgacgagt ctctcgcctt cctgcgccgc tggaacgaat acggcgccga
     2221 gattcagcaa aaatataaag accgcattat cgtcttcacc agcaccctgc cctgcggcgg
     2281 cgcgccgttt aacaaagaac tggaacgcgc cgtcaccgag tatggcttgc acggcgtgct
     2341 gatcaactcc agccaccagc atgcctatcc ggatgacgat gaagccatgc cgtttttcga
     2401 gttggtctcg agtctcggca tcccgatgat gatgcatgcg ccgtcggtgg gttacggcga
     2461 agagcgcatg cgcgactacc ggctggcatc gagcgtgggc cggccctttg acgagtgcct
     2521 gagcatcgcg cggcttatcg tccgcggcat cttcgagcgt cactcgaaat tgaaatttgt
     2581 cggctgtcat ttgggcggcg gtatctgcga cgtcatcggc cgcatggatt acgcctatga
     2641 gctaggcgat caggcgtcgg ggctaggcag ttatgaaccg atgttgatca cgaaaaagcc
     2701 cagcgagtat ttaaaaacca ttcacatgga tacggtcgcc taccacccgc cggcggtgat
     2761 gtgcgcctat cagaccgtcg gcgccagaca attgctcttc ggcagcgacg cgccgccgct
     2821 cctgccgctg ctgccgcgcg ccaaacagat catcgaagag ttgccgatcg gcgacgccga
     2881 acgggaagcg atcttcagcg gcaatgcatt gcgtttgctc ggtcgcgccg ggtaaaccgg
     2941 ttgacgtttc atgatggccg cgttaaagag ggcggaacag ctggaactga gtcctttctg
     3001 aaagattctc accgtgttag ttggaggcat gctcgccgtt aaattgaggt tcgctagcat
     3061 gccgtaggtc ccggcgcggt caccggtaag ccaaagcttt cgcgaattgt ttgacggtag
     3121 cgaaaaggag ttcaaaggat taatgttcta agtcaaagga cagtccacgg tggacaatgg
     3181 acaagatcac tgccggctcc agggcgatgc gcaaccagac gacgccaacc ccatcgtcat
     3241 ccgcgtcgac agcttcgatc ccgtggcgat cgatcaactg aaatattact ctttaagtac
     3301 aaggagaaca gcatggctgg actatcctac gacgatctga tcgaaccgac gatcgtcaaa
     3361 caagaatcaa aaagcgtcgc ctactgccgc tgcacgcgca gcaaaaatct gccgttctgc
     3421 gacggctccc acgagggcag caatatacag ccggcgatca tcgagctggc cagcccggaa
     3481 accatcgcta tctgccgctg ctggagatca aaagaccacc cttactgcga cggcacccac
     3541 ggccgcttgg taaaacccaa agagcgccca cccagggcgc acgggtaatg atagcatgtg
     3601 accgattgaa cccttgaacc ttgaactctt gaacgaatca ctgaaacatt ggcacaaaac
     3661 cgaggaggtt atttatggtc gaaatgaaaa atctagttaa agaagccgat acccaccctg
     3721 gcgttggcca tttgagtctc tggcgcacgg acttggcgta ttactgggtg atcaactgcg
     3781 aggccggcct gcaagacgac atgcactatc acgacaacga cgatcacatt ttcatgtgcg
     3841 tcgaaggtga gtgcaccgtg cgcacaccgc acaaagcctt cgtcctgaag cagttcgaca
     3901 ccgtgttgct gcaatccggc gagtcctacc aactgtgcaa taccggcaca ggccgcatga
     3961 ttcccgatga cagtgacggt cgccgcgacc ggtatgatcg ccaacgccaa ggccatgaga
     4021 cccatggcga atcccagcaa aatgtaggga agtaacagcg agagcgattc catgttcg
//
LOCUS       DASQKS010000597         6724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3280905, whole genome shotgun sequence.
ACCESSION   DASQKS010000597 DASQKS010000000
VERSION     DASQKS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3280905"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VE170_15280"
     CDS             <1..270
                     /locus_tag="VE170_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16859.1"
                     /translation="GFLLGLVDAIGETLRDTMIQLITPDRMRGRIKSFDQVFMSAGTY
                     LGHAQIGTAATFLGVPGALVMGGCLGSAGVLLVAHFAPGLREIDA"
     gene            complement(283..1329)
                     /locus_tag="VE170_15285"
     CDS             complement(283..1329)
                     /locus_tag="VE170_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16860.1"
                     /translation="MWFAGKESFISGVGGFIRRATLSVIGRAGTKMAAASLSLSVLLA
                     GANAQERLERVNLAIPVTALSQLPSYAGVRFGLFREEGLDVQIVLMNGRLVGPALLQG
                     DLDYSTLADTMMRAATTGLPVKVLAFGGIRPALALNVRPEIKTGADLKGKSIAVTSIG
                     STTDIVAREIVRSFGLNPERDIVTQGLGSQANNQTALRTGAVAAALFTPPYDVIAEKE
                     GFRVIAWAGDVIKEQLQAGLVTSDGKIARNPGQVKRMVRGFVKSLVFLRREKNKVVEM
                     ITKQWKIEPDIAEKSYLVMIKTFSANGSASDSAVENVIQQTLKANKSQKQVGHSQVAN
                     LAFLEEVQKELGLR"
     gene            complement(1396..1911)
                     /locus_tag="VE170_15290"
     CDS             complement(1396..1911)
                     /locus_tag="VE170_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16861.1"
                     /translation="MIAALVPTFPAHIAGQSRIDQRRDRGDDDIELAGEHCLQRHRTG
                     IDRNEFGVDSLSFHQTLIVDDPNHGVGRATGPGQAQAFLRLRRTSPQRCGQYQTASQC
                     HSSLLVYHRGFLQVPNQLRIALYRPDINLPRRQIQERFPTVRNAGRQPVCEESPSTTH
                     PIALTLYAPAR"
     gene            1940..2074
                     /locus_tag="VE170_15295"
     CDS             1940..2074
                     /locus_tag="VE170_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16862.1"
                     /translation="MKSYLDFLAPARSEAAKANANEFVDLSFVQEVQASGLIKQLYGR
                     "
     gene            2177..3019
                     /locus_tag="VE170_15300"
     CDS             2177..3019
                     /locus_tag="VE170_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ16863.1"
                     /translation="MPYATVRGVNINYQVLGNRGPWVALSPGGRRDMSGIELIAGEVA
                     KRGHRVVIFDRRNTGASDVVIDGNESEYEIWADDVHELLGQLGALPAVVGGSSSGCRT
                     SLLFALRHPESVRALLLWRVTGGRFACERLAEEYYGQFITAARSGGMAAVCAMDHWKE
                     RIAARPANRDALMKMDTASFIAVMSRWREYFLKGADLPVIGASDADLQSIPVPACIVP
                     GNDLTHGRQTGETLGRLLPTSEVHVLFPKHYDEPLSPREQWDEKAGEMAELFSDFLKR
                     QKIT"
     gene            3115..3795
                     /locus_tag="VE170_15305"
     CDS             3115..3795
                     /locus_tag="VE170_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ16864.1"
                     /translation="MIEPLDALKDKMVMVAKILQHQGVIDGYGHITARLPENKILSTP
                     HMPPGKVAVRDLIILDADGNKLDGFGAPNGETAMHTSIYKVRPDVQCILHYHADELVA
                     VAATGQEIKVISNCGVNFHRGTPIYDSPVLIRNPQLGDKVAATLSDKTAVLLRGHGGT
                     VVAHNLDQLCRLGLDLVRAAKIQIMAAALGAVKVHSKEECEAMAKIETRPDVHRRFID
                     YYVSEVVD"
     gene            3829..5262
                     /locus_tag="VE170_15310"
     CDS             3829..5262
                     /locus_tag="VE170_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ16865.1"
                     /translation="MAEQNPHASVPGGLTTAAAAQFVETITFEAIPADALRIGTRCLL
                     DGLGLFVAGSEEHTVQLLVEEAEQIGGRPDALLLSRGKTKVPAPMAARVLGTAGHAHD
                     WDDSQVSIDPAHVYGLLTHPTIPPLSSALVMAQKLGGIDGRAFMLAFLTGFEVECKIS
                     EWMLPQHYLRGFHSSGTVGTFGAFAAAAKLLRLKGDQLRSGFGIAASFAAGIRCNFGT
                     MTKPLHVGRAAENGVTAALLAARGFTADPDALDGPWGYFAVQGGGVSAEKVPQGFGKT
                     WTIVDPGVSIKPYPCGVLTHPTIDLMLRLVSDHDIRPDDIEAVKVYAGSNILNPIRYP
                     IAANHLQAKFSLPAALAMIALARKAGKKEFSDAFVAAPPMQAMQKRITTELDPAIEKM
                     GFDKMRSRIAIKVNGGKTIEGWADERYRGGPDNPLSDAELEAKVGSCCDGVLDQVRQA
                     KLIEAAWSVSKLPDAYRLMMLLNSIDD"
     gene            5320..6450
                     /locus_tag="VE170_15315"
     CDS             5320..6450
                     /locus_tag="VE170_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYJ16866.1"
                     /translation="MKIADIEIIELQNIPVTPPLFKQPLRTGVRLLKLKTDDGRVGLS
                     QIGGFLHAATIAAIKDELLPFLKGKDPLENERLMHQMLWKFNTRAHGGVWNYAVSAID
                     VALWDIKGKFYNTPVWRLLGGAQKAIPAYITFGLRAYNRDELAAAAQHWVANGQSRLK
                     VQVGRVNIRGESDPGGASAEHRSDNPAEDVARMRAVRDAVGDDVELMADANCLMKLDT
                     AVRWCKAFEPLNLTWFEEPIIHNDPHLLAQLRKQTHIPIAAGQWESFSRLADLVRADA
                     VDFLNIHVLSVGGFTMGMKAAGLAQAFNLPIGNGDHFDFHLHAAVPNGWRAEIHVNNW
                     LTANAVYKELPILADGWVTLNEKPGLGVELNDDAVKEFRKTA"
     gene            6536..>6724
                     /locus_tag="VE170_15320"
     CDS             6536..>6724
                     /locus_tag="VE170_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYJ16867.1"
                     /translation="MITIDVTTFKNRLGHYLRLVRRGNEIVITSHTQHVARILPYAMS
                     IGEIIEATRPVSDLMKVKG"
BASE COUNT         1461 a         2044 c         1886 g         1333 t
ORIGIN      
        1 ggatttttgc tcggtctagt cgacgccatc ggcgaaaccc tgcgcgatac catgatccag
       61 ctaatcaccc ccgaccgtat gcgcgggcgg attaaaagct tcgatcaagt cttcatgtcc
      121 gccggcacct acttgggcca cgcccagatt ggcacggcgg cgacttttct cggcgtcccc
      181 ggcgccttgg tcatgggcgg ctgtctcggc tccgccggcg tgttgctggt cgcccatttt
      241 gcgccgggtt tgcgcgaaat cgatgcctga tcgccaatgc ggtcagcgca gccccaactc
      301 cttctgcact tcttcgagaa aagccaggtt ggcgacttgg gaatgaccga cttgtttctg
      361 tgatttgttc gccttgagcg tctgctgaat gacattttca acggcgctgt cgcttgccga
      421 gccattggcg ctgaacgtct tgatcatcac cagatagctc ttctcggcga tatccggctc
      481 gatcttccac tgtttggtaa tcatctcgac aactttgttt ttttcccggc gtagaaacac
      541 cagactcttt acgaaaccgc gcaccatgcg ctttacctgg ccgggattgc gcgcgatctt
      601 gccatcggag gtcacgagac cagcttgcaa ttgctccttg atcacgtcgc ccgcccaagc
      661 gatgacgcga aaaccttcct tctcggcgat gacatcgtag ggcggcgtaa acaatgccgc
      721 cgccaccgcg ccggtgcgca gcgcggtttg attgttcgcc tggctgccca ggccttgggt
      781 gacgatgtcg cgctcgggat tgagaccgaa ggagcgcacg atctcgcgcg cgacaatatc
      841 cgtagtactg ccgatggaag tgaccgcgat actcttgcct ttcagatcag cgccggtttt
      901 gatctccggc cgaacgttaa gagccagcgc cggacgaatg ccgccgaagg cgagcacttt
      961 taccggcaac cccgtggtcg ccgcccgcat catcgtgtcg gccaacgtag aataatccaa
     1021 gtcgccttgc agcaacgccg gcccgaccag ccggccattc atcaggacga tctgcacatc
     1081 gagcccttcc tcgcgaaaca gaccgaaacg tacaccggcg taggacggca actggctgag
     1141 cgccgtgacc gggatggcga gattgactct ttcaagccgc tcttgtgcgt tggcgccggc
     1201 taacaatacc gaaagagata gacttgccgc cgccattttc gtgccggcgc ggccgatcac
     1261 cgagagggta gcccggcgga taaaaccgcc gacgccggaa atgaacgatt ctttgccagc
     1321 aaaccacatt gccttttcct ccgcagcgta atcggcaaat cgccaattca aaatccatag
     1381 ggttagtacc tatagttagc gcgccggcgc ataaagcgtc aaggcgattg ggtgcgttgt
     1441 cgatggggat tcttcacaaa caggttgtcg tcccgcgttt cggacggttg ggaatctttc
     1501 ttgaatttga cggcgtggaa gattaatgtc cgggcggtaa agggcaattc gaagttggtt
     1561 cggaacctgt aagaatccgc gatggtagac caacaggctc gagtgacatt ggcttgctgt
     1621 ttgatactgg ccgcaacgct gtggactggt acgccgcaag cgcaggaacg cttgcgcctg
     1681 gcctgggccg gtggcgcgtc caacgccgtg gtttggatcg tccacgataa gggtctgatg
     1741 aaaagacagg gagtcaacgc cgaattcatt acggtcaatg ccagtccgat ggcgttgcag
     1801 gcaatgctcg ccggcgagct cgatgtcatc gtcacctcgg tcacgacgct ggtcaattct
     1861 cgactgaccg gcgatatgag cgggaaaggt cggcaccagg gcagcaatca ttttcaagtc
     1921 ggcgccggcg ccggagggag tgaaaagtta tctcgatttt ctcgcgccgg cccggtcgga
     1981 agcggccaag gccaatgcca acgaatttgt cgacttgagc ttcgttcaag aagtccaggc
     2041 gtcgggactg atcaaacagc tctatggccg ataagcgcag tccacgcgaa ttttacaatt
     2101 ggcgttagcg cttgacgagt tgattcaaaa gctattcatc ggatagtgtc ggtgggtcga
     2161 agcctggagg tgctctatgc cctacgcaac agttcgcggt gtcaacatca actatcaagt
     2221 tctcggcaat cgcggcccct gggtggccct ctcgccaggc ggccggcgcg acatgagcgg
     2281 catcgagctg atcgccggcg aagtcgccaa acgcggccac cgcgtcgtca tcttcgatcg
     2341 gcgcaacacc ggtgcttccg acgtggtcat cgacggcaac gagtcggagt atgagatctg
     2401 ggccgacgac gtccatgaat tgctcggcca actcggcgcc ctacctgccg ttgtcggcgg
     2461 cagctcgtca ggttgccgta cttccctgct cttcgctctc cgtcatcctg agtccgtgcg
     2521 tgcgctcttg ctctggcgcg tcaccggcgg ccgcttcgcc tgcgagcgtt tagccgaaga
     2581 atactacggc cagtttatca ccgcagccag gagcggcggc atggccgcgg tgtgcgccat
     2641 ggatcactgg aaagaaagaa tcgccgcccg cccggcaaac cgcgatgccc tgatgaaaat
     2701 ggacacagcg agcttcatcg ccgtcatgtc acgctggcgc gaatactttc tcaaaggtgc
     2761 cgatttgccc gtcatcggcg cctccgatgc ggacttacaa tcgatcccag taccggcctg
     2821 tatcgttccc ggcaacgatc tcacccatgg acgacaaacc ggcgaaaccc tcggccgttt
     2881 gctgccaacc agtgaagtcc atgtgctgtt tcccaaacac tatgacgagc cgctcagccc
     2941 gcgcgaacaa tgggacgaaa aagccggcga gatggccgag cttttcagcg acttcctcaa
     3001 acgacagaaa atcacgtaaa cggacggggt tcggaaaggg gcgaaaaaca ttcgccgcca
     3061 catgccagat gaatcaacat ccccgataaa cgccaaacaa cctaaggaga caaaatgatc
     3121 gaaccgttag atgcactgaa agataaaatg gtgatggtcg ccaaaatact gcagcaccaa
     3181 ggggtgatcg acggctacgg ccacatcacg gcgcgcctgc cggaaaataa aatactcagc
     3241 acgccgcaca tgcctcccgg caaagtcgcc gtgcgcgatc tgattatcct cgacgccgac
     3301 ggcaataagc tcgacggctt cggcgcgccc aacggcgaga ccgccatgca tacctccatc
     3361 tacaaggtcc gtccggatgt tcagtgcata ctgcactacc acgccgatga gctggtcgcc
     3421 gtcgccgcca ccggtcaaga gatcaaagtg atctcgaact gcggcgtcaa ctttcaccgc
     3481 ggcacgccga tctacgactc gccggtgctg atccgcaacc cacaattggg tgacaaggtc
     3541 gcggccactt tgagcgacaa aaccgccgtc ctcctgcgcg gtcatggcgg caccgtggtg
     3601 gcgcacaacc tcgaccagct ctgccgcctc ggtctcgacc tcgtgcgcgc ggcaaaaatt
     3661 caaatcatgg ccgcagccct cggcgcagtg aaagtccatt ccaaggaaga atgcgaggcc
     3721 atggcaaaga tcgagacccg gcccgacgtc catcgccgct ttatcgatta ctacgtttcc
     3781 gaagtcgtcg attgattttt ttccaacgtc gatcccgaaa cctatcccat ggctgaacag
     3841 aatccccacg cgtctgtgcc cggcggtcta accaccgcgg ccgccgccca gttcgtcgaa
     3901 acgattacct tcgaggcaat tcccgcggac gccctgcgca tcggtacgcg ctgcctactc
     3961 gatggtttgg gtctgttcgt cgccggctcg gaggaacaca cggtccaatt gctcgtcgag
     4021 gaagcggaac aaatcggcgg ccgacctgat gcgctcctgc tcagtcgcgg caaaaccaaa
     4081 gtacccgcac ccatggcggc gcgcgtgctc ggcaccgccg gccacgctca tgattgggac
     4141 gacagccagg taagcatcga tcctgcccat gtctacggct tgttaaccca tccgacgatt
     4201 ccaccgttga gcagcgccct cgtcatggcg cagaaactcg gcggcatcga cggcagggct
     4261 ttcatgctcg ccttcctcac cggcttcgag gtcgaatgta aaatctccga gtggatgctg
     4321 ccgcagcatt atttgcgcgg ctttcactcc agcggcaccg tcggaacatt cggtgcattt
     4381 gccgcggcgg cgaaactctt gcgattgaaa ggtgatcaat tgcgcagcgg cttcggcatc
     4441 gccgccagct tcgccgccgg catccgctgc aacttcggca ccatgaccaa accgctccat
     4501 gtcggccgcg ccgcggagaa tggcgtgacc gctgcgctgc tcgcggcccg cggcttcacc
     4561 gccgaccccg atgccctcga tggtccctgg ggttacttcg ccgtccaggg cggcggtgtc
     4621 agcgctgaga aagtcccaca aggattcggc aagacctgga ccatcgtcga ccccggtgta
     4681 tcgatcaaac cttacccttg cggcgtgctc acccacccga ccattgattt gatgttgcgg
     4741 ctggtcagcg accacgacat aaggccggac gatatcgaag cggtaaaagt ctacgccggc
     4801 tcgaatatct tgaatcccat ccggtacccc atcgcggcca atcatctgca ggcaaagttt
     4861 tcgttgccgg cagcattagc catgatcgcg ctcgcccgca aagccggcaa gaaggaattc
     4921 tccgacgcat tcgtcgccgc accacccatg caagcgatgc aaaaacgcat caccaccgag
     4981 ctcgaccccg ccatcgaaaa aatgggcttc gacaaaatgc gctcgcgcat cgcgattaaa
     5041 gtgaacggcg gtaagaccat cgaaggctgg gccgacgaac gctaccgcgg cggccccgac
     5101 aatccgctga gcgacgcgga gttggaagcc aaggtaggtt cctgctgcga cggcgtctta
     5161 gatcaagtgc ggcaagcaaa actgatcgaa gccgcttggt ccgtcagcaa actaccggat
     5221 gcctatcggc tgatgatgct actcaatagt attgacgatt agtttgatgg gcgtgtctag
     5281 catggcaatg agtattcaag aatcacaagg agactacaca tgaaaatcgc cgacatcgaa
     5341 atcatcgagc tgcaaaacat tcctgtcacc ccgccgctgt tcaaacagcc attgcgcacg
     5401 ggagtgcggc ttttgaaact caagaccgac gacgggcgtg tcggcttgtc gcagatcggc
     5461 ggcttcctgc acgccgctac aatcgccgcg atcaaagatg agcttttgcc attcctgaaa
     5521 ggcaaagatc ccttggagaa cgagcgtttg atgcatcaga tgctatggaa gttcaacacc
     5581 cgtgcccatg gcggggtgtg gaattatgcc gtcagcgcca tcgacgtcgc actctgggat
     5641 atcaaaggca agttctataa cactccggtg tggcgactgc tcggcggcgc acaaaaagcg
     5701 atccccgctt acattacttt cggtctgcgc gcctataatc gggacgagct ggcggccgcg
     5761 gcgcaacact gggtggcgaa tgggcagagc cggcttaagg ttcaagtcgg ccgggtcaac
     5821 atccgcggcg aaagcgaccc gggcggcgcc agcgccgagc accgcagcga caatccggcc
     5881 gaagatgtcg cgcgcatgag ggccgtgcgc gacgcggtgg gcgacgatgt cgaattgatg
     5941 gccgatgcca attgtttgat gaagctcgac acggcggtgc gctggtgcaa agcattcgag
     6001 ccgcttaact tgacctggtt cgaagaaccg atcattcata atgatcctca cttgctcgcg
     6061 caactgcgaa agcagaccca tatcccgatc gctgccgggc agtgggaaag cttttcccgg
     6121 ctcgccgatc tggtgcgcgc cgacgctgtc gattttctca acattcacgt cctttcggtc
     6181 ggcggtttta ctatgggcat gaaggcggca ggattggccc aggcgttcaa tctgcccatc
     6241 ggtaacggcg accacttcga ctttcacctc cacgccgccg tgcccaacgg ctggcgcgcc
     6301 gagattcacg tcaataactg gctgacggca aacgcggtct acaaggaatt gccgatcctc
     6361 gccgatggtt gggtcacgct caacgaaaaa cccgggctgg gagtcgagct caatgacgat
     6421 gcggtgaagg agtttagaaa aactgcatag acaagagtgc cgcctacggc ccaacaataa
     6481 ctcccaactt gttcggcccg tgacgatcaa acaatttgaa agagatactc ggttcatgat
     6541 cacgattgat gtgactacct tcaaaaatcg tctaggtcac tatcttcggc tcgtgcgacg
     6601 tggcaacgaa atcgtcataa catcccatac ccagcacgtg gcgaggatac tgccatacgc
     6661 gatgtcgatc ggggaaatca tcgaagcaac ccgaccggtt agcgatctaa tgaaagttaa
     6721 agga
//
LOCUS       DASQKS010000598         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3703783, whole genome shotgun sequence.
ACCESSION   DASQKS010000598 DASQKS010000000
VERSION     DASQKS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703783"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            484..1002
                     /gene="tsaE"
                     /locus_tag="VE170_15325"
     CDS             484..1002
                     /gene="tsaE"
                     /locus_tag="VE170_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYJ16868.1"
                     /translation="MGGWTIVTTSPRQSQNLGRILGKLVQGGEIVGLVGEFGAGKTCF
                     VRGFARGIDVAADAWVRSPTFTLINEYSGRLPVYHIDLYRVGKQEELDSLNLREYLYG
                     DGVSLIEWFEFLPSHEVDEHLELRISHGGGDRRELTFIAHGQRYERLLEQLKSKALKR
                     SKRQKINDKKET"
     gene            1007..2218
                     /locus_tag="VE170_15330"
     CDS             1007..2218
                     /locus_tag="VE170_15330"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HYJ16869.1"
                     /translation="MALLVQKYGGSSVGSIDKIKGVAAKVAAAKRAGNDVIVVVSAMA
                     GETNRLIALAQEISQIPDEREKDVLIATGEQVTVALLSLAIKEIGQPARSYLGHQVRI
                     ETDNAYGKARILSIDSTKIVQALSAGEVVVIAGFQGVDENDNITTLGRGGSDTSAVAL
                     AAFLNARACEIYTDVEGVFTTDPRICPQARKLGKISYDEMIELASTGAKVLEIRSVEF
                     AKKYAVPVHVRSTFSPVEGTWLVEEDDSMSDVLVSGVACDKNEAKITLVRVPDQPGLA
                     AQIFGPIAAAHIVVDMIIQNASQDGTTDLTFTVPMADHQKALAIVEKSLPEVHAKGVR
                     VDTGIAKVSVVGVGMRTHAGVAAMMFETLAREKINIQMISTSEIKISVVIDAKQSDHA
                     VRALHEAFIAN"
     gene            2227..3807
                     /gene="cimA"
                     /locus_tag="VE170_15335"
     CDS             2227..3807
                     /gene="cimA"
                     /locus_tag="VE170_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HYJ16870.1"
                     /translation="MKDVLLYDTTLRDGCQAEDVSFTLEDKLRIAAKLDELGIDYIEG
                     GYPGSNERDADFFKRVKKLKLKNARIASFGTTMKVGIKPSQDFNLRVLLAADTPVVTL
                     VGKTWDLHVRDDLRISQKANLEIIADSIAYMKKRVDEVIFDAEHFFDGFRANPDYAIE
                     CLKAAQEGGADWIVLCETNGGRLPGEIREALTQVNRAVKTPLGIHCHNDGELAVANTL
                     AGVEMGVTQVQGTINGFGERCGNMNLISVIANLQLKMGKKCVTPAQLKKLREVSQLFY
                     ELANINPNKRQAYVGDSAFAHKGGLHVSGVIKNPETYEHIDPEVVGNRQRVLVSDLSG
                     RSNVVYKGKEYGIDLKDQNDAVKEVLRRTKELEGQGYEFQAAEASFELLIQEALGKKK
                     KNFNLIGFRVIDEKRKEGEAPISEATIQVGVDGEMEHAAAMGNGPVNALDQALRRALT
                     KFYPALKQVELLDYKVRVLSSGQGTAAVVRVLIESGDGKDRWGTVGVSHNVIEASWQA
                     LVDSIDYKLYKDARHRAN"
     gene            3916..4650
                     /gene="cysE"
                     /locus_tag="VE170_15340"
     CDS             3916..4650
                     /gene="cysE"
                     /locus_tag="VE170_15340"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HYJ16871.1"
                     /translation="MWQTIKEDFQVVSQRDPALRSVWEIVLAYPGFHAILLHRIAHAL
                     WGQGLTTLARFVSHVGRFLTGIEIHPGAQIGRRFFIDHGMGVVIGETAEIGDDVTIYQ
                     GVTLGGTSTQRIKRHPTIEDSVTIFSGAAVLGPVTVGRHSRIGAGSVLVTAVPAHSTV
                     VGIPGKVVKIEAHHTPTGKAIIDLNSADLPDPIAKALSAVGDYIEKLEKKVDELSARQ
                     GGLEERTAEETEELNRIKQTLRNTGS"
     gene            4690..>5036
                     /locus_tag="VE170_15345"
     CDS             4690..>5036
                     /locus_tag="VE170_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HYJ16872.1"
                     /translation="MNATKKKIVVAMSGGVDSSVTAALLVEQGHEVLGVSLRMWEGEK
                     GPRVCSDHRGAEEVARHLGISHTLLDLREQFANTVVKPFAQDYLHGRTPNPCIACNRD
                     VKLGTLLRWSEEQG"
BASE COUNT         1118 a         1449 c         1518 g          951 t
ORIGIN      
        1 aacaaagatc actttctcgt catcaaagaa aacaaactca ccaattatct ggcacggatc
       61 gcatgagagc ctttttttac gctctggggc cctccatcac gccggcaaag aaaacagtaa
      121 ctcctttctt aaagcgcgaa agcgcctaga ccaggcagtg gcttagaaga cgttgggctt
      181 atggtcttcg atccattttt tagcaaattc tagtcctttt ctttcggggt cgaccgctgt
      241 tgcaaagtat ttggttatca ctggatattt gaccgctagg ttacttccgt cactccacgt
      301 cacgtaaata cctagggccg tatctgcgac cgcccttttc aaaaagacat aaatcaccgg
      361 cccggcaagg agcagggaac gaaaccagcc gggataatgg aagggctcat accggtccgc
      421 cgtccccccg gctcgaaatc tctttaaaaa tatactagtt gtaatcggtg aggccgggtg
      481 cagatgggtg gctggactat cgttactact tcgccgcggc aaagccaaaa tctgggacga
      541 atcctgggaa aattagttca aggcggcgag atcgttggcc tggtcggcga gttcggcgcc
      601 ggtaagacct gctttgtccg cggttttgcg cggggcatcg acgtggccgc cgacgcctgg
      661 gtgcgcagcc cgacgtttac cttgatcaac gaatacagcg ggcgcttgcc ggtctaccac
      721 atcgatcttt accgggtcgg caagcaggaa gaactcgata gtctcaatct gcgcgagtat
      781 ctctacggcg acggcgtgag cttgatcgag tggttcgagt tcctgccgtc ccacgaagtc
      841 gacgagcatt tggagttgcg aatttcccac ggcggcggcg accggcgcga gctgaccttt
      901 atcgcccatg gacagagata tgagcgattg ctcgaacagt tgaaaagcaa agcgttaaaa
      961 agatcaaagc ggcaaaagat caacgacaag aaggaaacct gacagcgtgg cattactggt
     1021 gcaaaaatac ggcggctctt ccgtcggctc gatcgacaag atcaaagggg ttgcggcaaa
     1081 agtcgcggcg gcgaagcgcg ccggcaacga tgtcatcgtg gtggtttcgg ccatggccgg
     1141 cgagaccaac cggctgatcg ccttggccca ggagatttcg cagattcccg acgagcggga
     1201 aaaagatgtc ctgatcgcca ccggcgagca ggtgaccgtc gcgctcttga gtctggcgat
     1261 caaagagatc ggccagccgg cccgctctta tctgggccat caggtgcgca tcgagaccga
     1321 taacgcctat ggcaaggcgc gcatcctgag catcgactcg accaagatcg tccaggcctt
     1381 gagcgccggc gaagtcgtcg tcatcgccgg tttccagggc gtcgatgaaa acgacaatat
     1441 tacgaccctg gggcggggcg gctcggacac cagcgcggtg gcgctggcgg cgtttctcaa
     1501 tgccagggcc tgtgagattt ataccgacgt cgaaggcgtc tttaccaccg atccgcggat
     1561 ctgcccgcag gcgcgcaagc tcggcaagat ttcctacgat gagatgatcg agctcgccag
     1621 caccggcgcg aaagtcctgg agattcgttc cgtcgagttc gcgaaaaaat atgccgtgcc
     1681 ggtgcatgtc cgttcgacgt tttccccggt cgaaggcacc tggctggtcg aggaggatga
     1741 cagcatgagc gatgtattgg tttccggagt ggcctgcgac aagaacgaag ccaagattac
     1801 cctggtccgg gtgcccgatc agcccggcct ggcggcgcag atcttcggcc cgatcgcggc
     1861 ggcgcatatc gtcgtcgaca tgataataca aaatgccagc caggacggca ccaccgatct
     1921 gactttcacc gtgcccatgg ccgatcatca aaaggccctg gccatcgtcg aaaagtcgct
     1981 gccggaagtc catgccaaag gcgtgcgggt cgataccggc atcgccaagg tttccgttgt
     2041 cggcgtcggc atgcgcaccc atgccggcgt ggcggcgatg atgttcgaga ccctggcgcg
     2101 ggagaagatc aatattcaaa tgatctcgac ttcggagatc aagatttccg tggtcatcga
     2161 cgccaagcag tcggaccacg ccgtgcgcgc gctgcacgag gccttcatcg ccaattagag
     2221 gaagttatga aagacgtttt actttacgac acgacactgc gagacggctg ccaagccgaa
     2281 gatgtttcgt ttaccctgga agacaagttg cgcatcgccg ccaaattgga cgagctcggc
     2341 atcgattata tcgaaggcgg ttatcccggt tccaacgagc gcgacgcgga ctttttcaag
     2401 cgggtcaaaa aactcaagct caagaatgcc aggatagctt ccttcggcac caccatgaag
     2461 gtcggcatca agccgtccca ggatttcaac ctgcgcgtgc tgctggccgc cgacacgccg
     2521 gtggtgaccc tggtcggcaa gacctgggac ctgcatgtgc gcgacgatct gcgcatcagt
     2581 caaaaggcca acctggagat catcgccgac tccatcgcct acatgaaaaa gcgggtcgac
     2641 gaagtgatct tcgacgccga gcatttcttc gacggctttc gcgccaatcc ggactacgcc
     2701 atcgagtgtc tcaaggcggc ccaggaaggc ggcgccgact ggatcgtcct gtgcgagacc
     2761 aatggcgggc gcctgccggg cgagattcgc gaagcgctga cccaggtcaa ccgcgcggtc
     2821 aagacgccgc tggggattca ctgccataac gacggcgagc tggcggtggc caatactctg
     2881 gccggcgtcg agatgggcgt cacccaggtg cagggcacca tcaacggttt cggcgaacgc
     2941 tgcggcaaca tgaatctgat ttccgtgatc gccaacctgc agctcaagat gggcaagaaa
     3001 tgcgtcaccc cggcgcagct gaaaaagctc cgcgaggtct cgcagctgtt ctacgagctc
     3061 gccaacatca accccaacaa gcggcaggcc tatgtcggcg acagcgcctt cgcccacaag
     3121 ggcggcctgc atgtctccgg cgtgatcaag aacccggaga cctacgagca tatcgatccc
     3181 gaagtggtcg gcaatcgcca gcgggtcctg gtctcggatc tgtccggccg cagcaacgtg
     3241 gtttacaagg gcaaggaata cggcatcgat ttgaaagatc agaacgatgc cgttaaagag
     3301 gtgttgcgcc gcaccaagga gctcgaaggc cagggctacg agtttcaggc ggcggaagct
     3361 tccttcgaac tgctgatcca ggaagcgctc ggaaagaaga agaaaaattt caatctcatc
     3421 ggctttcgcg tcatcgatga gaagcgcaag gaaggcgagg cgccgatttc cgaagcgacg
     3481 attcaagtcg gggtcgacgg cgagatggaa catgcggcgg ccatgggcaa cggcccggtc
     3541 aatgcgctgg accaggcgct gcgccgggca ctgaccaaat tttacccggc gctcaaacaa
     3601 gttgagttgc tcgactacaa agtccgcgtg ctgtcctccg gccagggaac cgccgcggtg
     3661 gtgcgggtgc tgatcgaatc gggcgacggc aaggaccgct ggggcaccgt gggggtgtcg
     3721 cacaacgtca tcgaggcgag ctggcaggcg ttggtcgaca gtatcgatta taagttgtac
     3781 aaggacgcgc ggcatcgggc gaactaaaag attggagtga tggagtagtg gagtaatgga
     3841 gtgatgggaa gaacgggccg attccgaccc aatattccat cactccaaca ctccatttac
     3901 ccattccccg ttcccatgtg gcagaccatc aaagaagatt tccaggtggt ttcgcagcgc
     3961 gatccggcgc tgcgctcggt ctgggagatc gttctggcct atccgggatt tcacgccatc
     4021 ctgctgcacc gcattgccca tgcgctgtgg ggccaggggc tgaccaccct ggcgcgcttt
     4081 gtcagccacg tcggccgctt tcttaccggt atcgagatcc acccgggcgc gcagatcggc
     4141 cggcgctttt tcatcgatca cggcatgggt gtggtcatcg gcgagaccgc cgagatcggc
     4201 gacgatgtga ccatctacca gggcgtcaca ctcggcggca cttccaccca gcgcatcaag
     4261 cgccatccga ccatcgaaga ttcggtgacg atcttcagcg gcgcggcggt tctcggcccg
     4321 gtgacggttg gccggcacag ccggatcggc gccggctcgg tgctggtgac ggcggtgccg
     4381 gcccattcca cggtggtcgg cattcccggt aaagtggtca aaatcgaggc gcaccatacg
     4441 cccaccggca aggcgatcat cgatttgaat tccgccgacc tgcccgatcc catcgccaag
     4501 gcgctgagcg cggtgggcga ctatatcgaa aagctggaaa agaaagtcga cgaactgagc
     4561 gcgcgccagg gcggtctcga agagcgcacc gccgaagaaa ccgaagagct caatcgcatc
     4621 aaacaaacgc tgcgcaatac cggcagctaa atttaaccgt tcaaaacgtt caattcgttt
     4681 cgtgcctcga tgaatgccac aaagaaaaaa atcgtcgtcg cgatgagcgg cggcgtcgat
     4741 tcttcagtca ccgcggcgct gctggtggag cagggccacg aagtcctggg cgtttcgctg
     4801 cgcatgtggg agggcgagaa agggccgcgg gtgtgctcgg atcatcgcgg cgccgaagaa
     4861 gtcgccaggc atctgggcat ttcgcatacc ttgctcgacc tgcgcgagca attcgccaac
     4921 acggtggtca agccgtttgc ccaggattat ctgcacggcc gcacgcccaa tccctgcata
     4981 gcctgcaatc gcgacgtcaa gctcggcacg ctgttgcgct ggagtgaaga acaggg
//
LOCUS       DASQKS010000599         8847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6231954, whole genome shotgun sequence.
ACCESSION   DASQKS010000599 DASQKS010000000
VERSION     DASQKS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6231954"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VE170_15350"
     CDS             <1..772
                     /locus_tag="VE170_15350"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYJ16873.1"
                     /translation="QGYRYYIDCLRHHMGTAGMLRIDHVMGLHRAFWVPDGFSATDGV
                     YVHHRAPEFYAILNLESHRHQVQIVGENLGTVPAQVNQTMAQRRFLGMHVGQFGVSTD
                     PDNALSAVPTDVVASLNTHDTATFMGFWQGDDIDDHVALGLASADQADQERRYRRAQK
                     DALIAYLRAHGHMGDDFSPSAVLAGWLGFLAGQSGEFLLINLEDLWLEATPQNVPGTW
                     MERPNWQRKARYSLTEIRDRATFNELLKTVSDIRAGMV"
     gene            782..2692
                     /gene="glgB"
                     /locus_tag="VE170_15355"
     CDS             782..2692
                     /gene="glgB"
                     /locus_tag="VE170_15355"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYJ16874.1"
                     /translation="MSESAPFSLLSAHDSYLFNEGSHFRLYDKLGARLVSVNGTNGCY
                     FAVWAPNAERVSLVGDFNGWNPSTHPLKAKGSTGIWETFVPGIGKGTLYKFHVESRFN
                     GYRADKADPFALFNEIPPKSASIVWDLEYDWQDRDWVARRQQKNRLDRPMAIYEIHLG
                     SWRRVAGEGNRSLSYRELAPLLADYIEQLGYTHVELLPVMDHPFFGSWGYQITGYFAP
                     SGNFGTPQDLMFLIDTLHQRGIGVILDWVPSHFPSDEHGLAYFDGTHLFEHADPRQGY
                     HPDWHSYIFNYGRHEVQSFLISNALFWLDKYHIDGLRVDAVASMLYLDYGRKNGEWIA
                     NRYGGRENLDAIELLRRLNHVVYENYPDVQTIAEESTDWPMVSRPVHLGGLGFGLKWD
                     MGWMHDTLEYFHRDPVHRRYHQSNLTFRMIYAFHENFVMPLSHDESVHGKGSLLEKMA
                     GDDWQRFANLRALYGYMYAQPGKKLLFMGGEIAQRREWDHDAGLDWNLLDAPAHAGVQ
                     RWVRDLNQLYRNEPALHELDCEPAGFEWIDCDDAEGSVVSLLRKGKSTASLVLVLCNF
                     TPVPRDGYRLGVPRRGYWREALNSDALEYGGSGIGNRGGLDADPVAHHGRPFSLVLTL
                     PPLSVSFFVNQP"
     gene            2708..4147
                     /gene="glgA"
                     /locus_tag="VE170_15360"
     CDS             2708..4147
                     /gene="glgA"
                     /locus_tag="VE170_15360"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HYJ16875.1"
                     /translation="MKIAMIVSEANPFAKTGGLADVSAALSLALERLGHQVTLIIPAY
                     RSALQGGFKLQDSGTNFSVPLSDRLEPAAVLQSKIGDAVTVYIIRADKYFDREFLYGT
                     ATEDYPDNAERFVFFCRAASEILRAQPADIVHAHDWQAALAIVFLKTQAARYDTAAAA
                     KTVLTIHNLAFQGNFSPTAWHFLNLDNAFFTPQFLEFYGNVNFLKGAIVLADKITTVS
                     PTYAQEVMTLEQGFGLDGALRQRAGDLVGILNGVDYGQWNPATDPSISQHYDELSLTN
                     KRHCKESLRQQVGLPDKANTPIVAMISRLTAQKGFDLVQMKFPELIQRDLQFVLLGSG
                     EPRYEEFFRGAAASYPDRVAVRIGFDEQLAHRIEAGADLFLMPSLYEPCGLNQMYSLK
                     YATLPIVRAVGGLKDSVQDYDPHNQNGNGFVFTDYDGDAMLAAIDRGLSAYGDKAAWT
                     ALQQRAMAMDLSWQRSAQLYNELYQSLLP"
     gene            4169..5419
                     /gene="glgC"
                     /locus_tag="VE170_15365"
     CDS             4169..5419
                     /gene="glgC"
                     /locus_tag="VE170_15365"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HYJ16876.1"
                     /translation="MLYVLGVVMAGGRGERLYPLTQHRAKPAVPFGGRYRIIDFVLSN
                     FINSGILSVYVVTQFKAQSLLEHLDRGWRATDFLGEHFITPVPAQMRSGDDWYQGTAD
                     SVYQNLYLLERHKPTLVAVFGADHIYRMNVRQMIDAHKNQSAEVTVATVPVNIAEASQ
                     FGVVEIDANWRIIGFEEKPKEPKSIPGEPDFALVSMGNYLFNNDVLIDALKADDANPA
                     SNHDFGRDLLPGFVAGGRMFAYDFRQNHVPSPSKGEEPSYWRDLGTIEAYYDASMDLC
                     SIDPSFNLYNRSWPLRTVGYADPPAKFVFDGADRQGMAVNSVVAEGTIISGSDVRNCI
                     VGRNVRVHSYSHIENSVIMDWVEIGRNCRIRNAIIDKANVIPSGTEIGYDLEKDRQRY
                     FVSPSGIVVLGRGPRKTTWVMTNS"
     gene            5471..7894
                     /locus_tag="VE170_15370"
     CDS             5471..7894
                     /locus_tag="VE170_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HYJ16877.1"
                     /translation="MERYICIHGHFYQPPRENPWLEAIELQDSAYPYHDWNERITAEC
                     YAPNSVARIVDSDNRIIKLINNYARISFNFGPTLLSWLEAKAPGVYGTILDADRESQQ
                     RFSGHGSALAQVYNHAIMPLANSRDRKTQILWGIGDFTNRFRRAPEGMWLAETAVDVE
                     TLELLAAQDIQFVILAPHQAAKVRAIGERQWKAIHGDAIDPSRAYRQVLPSGRVIALF
                     FYDGPIARAVAFEGVLSHGDRFVDKLMGGFDDKRDWPQLVHIATDGESYGHHHRFGDM
                     ALAYALDRIETKQPALLTNYGEFLERHPPAHEVEIAEKTSWSCAHGIDRWWSDCGCNS
                     GAHGGWNQQWRTPLRNALDGLRDAVTPLWQQQATDYFKNIWFARDTYIEVILDRSAAS
                     REQFLARHAARPLTGDEKIRALKLLELQRQLLLMYTSCGWFFDDISGIETVQVLQFAG
                     RAVQLAQELFGDSMEERFIERLGAAHSNLQDQGDGKQIYEQRVRPARVDWERVGAHYA
                     VSSLFETIPEQTKIYCYHAERKNLQTYAAGNAKLAVGEVELTSEITGETTCLSFGALH
                     LGDHNVNAGVRPSLGDNIDSYLARELAEPFMQGDFPTVLRLMDRRFGESNYSLRSLFH
                     DEQRKLIGQILQSALSETESFYRQIYDHRAPLMRFLHSLDLPQPSALRGPAEAVINSD
                     LRRQLEKDDIDSERLQQLLAAAIAEGIALDSATLEFTYRKTLERSAQAFVNQPTLTAL
                     QSLNQAATAIAMLPFAVNLWKVQNLLYRFIERFHAETSQATQWRADDRDQWYTCLREL
                     GEKLAVKVS"
     gene            complement(8093..>8847)
                     /locus_tag="VE170_15375"
     CDS             complement(8093..>8847)
                     /locus_tag="VE170_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16878.1"
                     /translation="KTTMILESTALASVEPRRSDAKSAAPSAGTDNGVEAATTKRSYA
                     NVTGNLSKDPSAPTPSAAPAKVFSLWQSASFEFGDFLDIVNPLQHIPIVATIYRNLTD
                     DRIGMAPRVIGGALWGRVTGLVTGVINSLVEWFTGKDIGDHIYAKIWGEPANPNILAR
                     AAQLVSEKSPMAAAAAPELITATVPEPVARPDEPARGLSEAYQTPATTTTAIPPTVLP
                     EFWPRPFHHLPYRLGDDFGEPQDGIGKLNISA"
BASE COUNT         1861 a         2681 c         2463 g         1842 t
ORIGIN      
        1 gcagggttat cgctactaca tcgattgcct gcgtcatcac atgggcacgg ccgggatgtt
       61 gcgcattgac catgtcatgg gtctgcatcg cgccttctgg gtgccggacg ggtttagcgc
      121 cacagacggc gtctacgtgc accatcgcgc gccggagttc tacgcgattt tgaatttaga
      181 atcgcaccgc catcaagtgc agatcgtcgg tgaaaacctc ggcacggttc ccgcgcaagt
      241 taaccaaacg atggcgcagc ggagatttct cggcatgcat gtcggccagt tcggcgtgag
      301 caccgacccg gacaacgcgt tgagcgccgt gcccaccgat gtggtcgcca gtctaaacac
      361 ccatgatacg gcaaccttca tgggcttttg gcaaggcgat gatatcgacg accatgtcgc
      421 cctgggactc gccagtgccg atcaagccga ccaagaacgg cgctatcgca gggcgcagaa
      481 agatgccctg atcgcctatt tgcgcgccca cggccatatg ggcgacgatt tttcgcctag
      541 cgcggttctc gctggctggc tcggttttct cgccggacaa agcggcgagt ttctattgat
      601 caatttggaa gatctctggc tcgaagccac gccgcaaaat gttcccggca cctggatgga
      661 gcgccccaac tggcagcgca aggcccgtta ttctttgaca gaaatccgtg accgagcgac
      721 gtttaacgag ctgctaaaga cagttagcga catccgcgct gggatggttt agagtaccag
      781 gatgtccgaa tcggccccgt ttagcctgct cagcgcacac gacagctacc ttttcaacga
      841 aggcagccac tttcgcctct acgacaaact gggcgccaga ttggtcagcg taaacggcac
      901 gaacggctgc tatttcgcgg tctgggcgcc caacgccgaa cgggtttcgc tcgtcggcga
      961 cttcaatggc tggaacccat ccacccatcc gctcaaagcc aaaggttcaa ccggaatctg
     1021 ggaaacattt gtccccggca tcggcaaggg caccttgtat aaatttcatg tcgagtcgcg
     1081 cttcaacggc taccgcgccg acaaagccga ccccttcgcc ttgttcaatg agataccgcc
     1141 gaagagcgcg tctatcgtct gggatctcga atacgattgg caggaccgtg actgggtcgc
     1201 ccggcgccag caaaaaaacc ggctcgaccg gccgatggca atttatgaaa tccacctggg
     1261 ttcatggcgg cgcgtggccg gcgagggcaa ccgctcgctg tcctaccgag agctggcgcc
     1321 gcttttggcc gactatatcg agcaactcgg ctacacccat gtcgaattgc tgccggtgat
     1381 ggaccatccg ttctttggct cgtggggtta tcaaatcacc ggctacttcg ctccctcggg
     1441 gaatttcggc acgccccaag acttgatgtt tttgatcgat accctgcacc agcgtggcat
     1501 cggcgtcatc ctcgactggg tgccgtccca tttccccagc gacgagcatg ggctcgccta
     1561 cttcgacggc acgcacctgt tcgagcatgc cgatccgcgc cagggctatc atcccgattg
     1621 gcacagttac atcttcaact acggccgcca cgaggtacaa agcttcctga ttagcaacgc
     1681 gcttttttgg ctcgacaaat atcatattga cggcttgcgc gtcgatgcag tggcctccat
     1741 gctctatctc gactacggcc ggaaaaatgg cgagtggatt gccaatcgat acggcggccg
     1801 ggaaaatctc gacgccatcg aactgctgcg ccggctcaat cacgtggtct acgaaaatta
     1861 tccggacgtc caaaccatcg ctgaagaatc caccgactgg ccgatggtgt cgcgcccggt
     1921 ccatttgggc ggcctcggct tcggcctcaa atgggacatg ggctggatgc acgacacgtt
     1981 agaatacttt cacagagacc cggtgcaccg tcgctaccat caaagcaatc tcacctttcg
     2041 catgatctac gcctttcacg agaactttgt catgccgctg tcgcacgacg aatcggtcca
     2101 tgggaaaggc tcgctgctcg agaaaatggc tggtgacgat tggcagaggt tcgccaatct
     2161 gcgcgcgctc tacggctaca tgtatgccca gccgggcaag aagctgcttt ttatgggcgg
     2221 cgaaatcgct cagcggcgcg agtgggacca tgacgccggt ctcgattgga atctgctcga
     2281 tgccccggcc catgccggtg tgcagcgctg ggtgcgcgat ttgaaccaac tctaccgcaa
     2341 cgagccggcg ctccatgagc tcgactgcga gcccgccggc tttgagtgga tcgattgcga
     2401 cgacgccgag ggcagtgtcg tcagcctgct gcgcaagggt aaatcgactg ccagcttagt
     2461 gctcgtcttg tgcaacttca ctcccgtgcc ccgcgatggt tatcgcctcg gcgtgccgcg
     2521 gcgcggttat tggcgcgagg cgttaaacag cgacgccctg gaatatggcg gcagcgggat
     2581 cggcaatcgc ggcggtctcg acgctgaccc ggtggcgcac catggccggc cattttcctt
     2641 ggtgctgacc ttgccgccgt tgtcggtgtc gttttttgtc aaccagccat agttagtttt
     2701 cgctgccatg aagatcgcca tgatcgtttc cgaggccaac ccgttcgcca agaccggcgg
     2761 cctggccgat gtctcggccg cactgtcctt ggcgctggag cggctcggcc accaagtcac
     2821 gctgattatc ccggcttacc ggagtgcgtt gcagggcggc tttaagctgc aggacagcgg
     2881 cacgaacttt tccgtacccc tatccgaccg tctggaaccg gcagcggtgc tgcagagcaa
     2941 aatcggcgat gcagtcactg tttatattat ccgtgccgat aaatatttcg accgtgagtt
     3001 tctctacggc acagcaaccg aagattatcc cgacaatgcc gagcgctttg tcttcttttg
     3061 ccgggcggcg tcggaaattc tccgcgccca gccggctgac atcgttcacg cgcacgactg
     3121 gcaagccgcc ttggccatcg tatttttgaa gacccaagcc gcgcgctatg ataccgccgc
     3181 cgctgcgaaa accgttttga ctatccataa cttggccttc cagggaaatt tttcgccgac
     3241 cgcctggcat tttttgaatc tcgataacgc tttttttacg ccgcagtttt tagaattcta
     3301 cggcaacgtc aatttcctca aaggcgctat cgtattagcc gacaaaataa ccaccgtgag
     3361 tccaacctac gcccaggagg ttatgaccct cgagcaaggc ttcggcctcg acggagcgct
     3421 gcggcagcgt gccggcgatc tcgtcggcat cttgaacggc gtcgactatg gccaatggaa
     3481 tcctgcgacc gacccatcga tttcgcaaca ctacgacgaa ctcagtttga ccaacaaacg
     3541 ccactgcaaa gaatccttgc gccaacaggt cgggctaccg gataaagcca atacgccgat
     3601 cgttgcgatg atctcgcggc tgaccgcgca aaagggcttc gatctggtgc aaatgaaatt
     3661 tcccgaactg atccagcggg acctgcaatt cgtcctactc ggcagcggcg agccgcgcta
     3721 cgaagagttt tttcgcggcg cggcggcgag ctacccggat cgcgtcgccg tgcgcatcgg
     3781 ctttgacgag cagctggcgc atcggattga agccggcgcc gatctattct tgatgccgtc
     3841 gctttacgag ccctgcggct tgaatcaaat gtacagtttg aaatacgcca ccttgccgat
     3901 cgtccgggcc gtcggcgggc tgaaagattc cgtgcaggac tacgatcctc acaaccagaa
     3961 cggcaacggt ttcgttttta ccgattacga tggcgacgcc atgctcgccg ccatcgaccg
     4021 cggtttgtcc gcctacggcg ataaagcagc ttggaccgcg ctgcagcaac gggccatggc
     4081 aatggatttg tcctggcaac gctcggcgca actttataac gaattatatc aatccctgct
     4141 gccgtgacgg cgggatggag taagagaaat gttgtacgta ctaggtgtgg tcatggcggg
     4201 cgggcgcggc gagcggctct accccctgac ccagcatcgg gccaagccgg cggtgccctt
     4261 cggcggccgt tatcgaatta tcgatttcgt gctgtcgaac ttcatcaatt cgggaattct
     4321 ctcggtttac gtcgtcaccc aattcaaagc gcagtcgctc ttggaacatc tcgaccgtgg
     4381 ctggcgggca acagattttt taggcgaaca ttttatcacg ccggtgccgg cgcagatgcg
     4441 ctcgggcgac gactggtatc agggcaccgc cgattcggta tatcagaatc tctacttgct
     4501 tgaacggcac aagccaacgc tggtggcagt gttcggcgcc gaccacattt atcggatgaa
     4561 tgtccggcaa atgatcgacg cgcataaaaa tcagtcggcc gaagtcacag tggcgaccgt
     4621 tccggtgaac atcgcagaag cctcgcagtt cggcgtagtc gagatcgatg ccaattggcg
     4681 gataatcggt ttcgaagaaa aacccaagga acccaagtcg attcccggtg agcccgattt
     4741 cgccctggtg tccatgggga attatctatt caacaatgac gtgttgatcg acgccttgaa
     4801 agccgacgac gccaatccgg cgagcaatca tgattttggc cgcgatctgc ttcccggttt
     4861 cgtcgcaggc gggcgcatgt tcgcctatga ctttcgccag aaccacgtgc ccagcccttc
     4921 caagggcgaa gagccgagct actggcgcga cctcggcacc atcgaagcct actacgacgc
     4981 cagcatggat ctgtgctcca tcgatccgtc gttcaattta tataaccgca gttggccgct
     5041 gcgcacggtg ggttacgccg atccgccggc aaaatttgtc ttcgacggcg ccgaccgcca
     5101 aggcatggcg gtcaattcgg tggtcgccga aggcacaatt atcagcggca gtgacgtccg
     5161 caactgcatt gtcggacgta atgtgcgcgt ccatagctac agccatatcg aaaactcggt
     5221 catcatggat tgggtggaaa tcggcaggaa ctgtcgaatc cgcaatgcca tcatcgacaa
     5281 ggccaacgtg atcccgtccg gcacggagat cggctacgac ctggaaaaag atcgccagcg
     5341 ctacttcgtg tcgccgagcg gcatcgttgt cctcggccgt ggaccgcgca aaaccacctg
     5401 ggtcatgact aattcctgac cgaagtcttc gtcaagccgc agcaggatcc gccgaaagat
     5461 cgatcgcacc atggaacgct acatctgcat acacggccat ttctatcaac cgccgcggga
     5521 aaacccctgg ctcgaggcta ttgagctgca agactcggcg tatccgtacc acgattggaa
     5581 cgagcgcata acggcggaat gttacgcgcc caactccgtg gcgcgaatcg tcgatagcga
     5641 caatcgcatc attaaactaa tcaacaatta cgcgcgcatc agtttcaatt tcggcccgac
     5701 cttactgtcc tggctcgagg ccaaagcgcc gggcgtatat ggcacgatcc tcgacgccga
     5761 ccgtgaaagt cagcagcgct tctccggcca cggctcagca ctggcgcagg tctacaatca
     5821 tgcgatcatg ccgctcgcta atagccggga ccgcaagacc cagatactct ggggcatcgg
     5881 cgatttcacc aaccgttttc ggcgcgcccc ggaaggtatg tggctggcag aaaccgccgt
     5941 cgacgtggaa accctggaat tgctggccgc acaggatatt cagttcgtca tcttggcgcc
     6001 gcaccaggcc gccaaagtgc gcgccatcgg tgaacgccaa tggaaagcga tccatggcga
     6061 cgccatcgat cccagccgag cttaccgcca agtgctgccg tcaggacgcg tcatcgcgct
     6121 atttttctac gacggaccga tcgcccgcgc ggtcgccttc gaaggggtgc tatcccacgg
     6181 cgaccgcttt gtcgacaaac tcatgggcgg ctttgacgat aaacgcgatt ggccccagct
     6241 ggtccatatc gccaccgatg gcgaaagtta tggccatcat catcggtttg gcgatatggc
     6301 gctggcctac gcactggatc gaatcgaaac caaacagccg gcgctcttga ccaactatgg
     6361 tgagtttctc gaaagacatc cgcccgccca cgaagtcgaa atcgccgaaa aaacttcctg
     6421 gagttgcgcc catggcatcg accgttggtg gagcgattgt ggttgcaact ccggcgccca
     6481 tggcggctgg aaccaacagt ggcgcacacc gctgcgcaac gccctcgacg gcctgcgcga
     6541 tgcggtcaca ccactttggc agcagcaagc cacggattat ttcaaaaata tctggtttgc
     6601 ccgggatact tacatcgaag tgatcctcga ccgctcggcg gcatctagag aacaatttct
     6661 cgcccgtcac gcagctcggc cgttgaccgg cgatgaaaaa atccgcgcgc tcaaattgct
     6721 cgaactgcag cggcaactgt tgctcatgta caccagctgt ggctggtttt tcgacgacat
     6781 ctcgggcatt gagaccgtcc aagtcctcca gttcgccggc cgcgccgtcc agttagctca
     6841 agagcttttt ggcgactcaa tggaagaacg ctttatcgag cgactcggcg cggcgcacag
     6901 caacctgcaa gaccagggtg acggtaaaca gatttacgag caacgggtcc ggcccgcccg
     6961 tgtcgactgg gagcgggtcg gcgctcatta tgccgtcagt tcgctgttcg aaaccatacc
     7021 ggaacagact aaaatctatt gctaccacgc cgaacgaaaa aacctgcaga cttacgctgc
     7081 cggcaacgcc aagctggccg taggcgaagt cgagctcacc tcggaaatca ccggcgagac
     7141 cacttgcctc agcttcggcg cgctgcacct gggcgatcat aatgtcaacg ccggcgtgcg
     7201 cccatccctt ggcgacaaca tcgatagtta cctcgcccgc gagctcgccg agcccttcat
     7261 gcaaggtgat ttcccgacag ttttgcgtct aatggatcgc cgcttcggcg aatccaacta
     7321 ctctctgcgt tcgctgttcc acgatgaaca gcgcaaactg atcggccaga tcttgcaatc
     7381 cgccttaagc gaaaccgagt cgttctaccg gcaaatctac gaccaccgcg ccccgctcat
     7441 gcgtttttta catagcttgg acttgccgca accgagcgcg ctccgcgggc cggcggaggc
     7501 ggtcatcaac agcgacctgc gccgccagct ggaaaaagac gacatcgaca gcgagcgctt
     7561 gcaacaatta cttgccgcgg ccatcgccga gggcatcgcg ctcgacagtg cgacgttgga
     7621 atttacctac cggaaaaccc tcgaacgaag cgcgcaagcc ttcgtcaacc aaccaaccct
     7681 caccgcgctg cagagtttga accaagccgc cacggcgatc gccatgctgc cattcgcggt
     7741 caatttgtgg aaagtgcaaa atctgctcta tcgcttcatc gagcgcttcc atgccgaaac
     7801 ttcacaagcc acccagtggc gcgccgatga tcgcgaccag tggtatacct gcctgagaga
     7861 gctcggcgaa aaactcgccg ttaaggtctc ctgaccggat agcctagcga agttccgccg
     7921 cgataaaatt gccggtagcg ttatcgtttc gaacggggag aataatccgt cttagatttt
     7981 cgcgctggac atcacgagcc tcggcgcgtc actttgcgag cttagcgtgc cgatcgcaga
     8041 aagtacttca ctactccgga gagactacga agaacctggg gcgctccact tactaggcac
     8101 tgatgttgag tttaccgatg ccgtcctgcg gttcgccgaa atcatcgccg agacgataag
     8161 gcaaatgatg gaatgggcgc ggccagaact ccggcagcac cgtcgggggg atggcggtgg
     8221 tggtcgttgc gggtgtctga taagcctcgc ttaaaccgcg cgctggctcg tccggccgcg
     8281 ccaccggctc aggcactgtc gcggtgatta attctggggc agcagctgcc gccatcgggc
     8341 tcttttcgct cactagctgg gccgcccgcg ccagaatgtt tggattggct ggctcacccc
     8401 aaatcttggc gtagatatga tcgccgatat ccttaccggt gaaccattcg acaagtgaat
     8461 taatcactcc ggtcaccaag ccggtcactc ggccccaaag cgcgccgccg atcaccctcg
     8521 gcgccatgcc gatgcgatcg tccgttagat tacgataaat cgtcgcgaca atcgggatat
     8581 gctggagtgg attgacgatg tcaaggaagt cgccaaattc aaaacttgca ctttgccaca
     8641 gcgaaaaaac ttttgcgggt gcggcgctcg gagttggcgc gctgggatct tttgagaggt
     8701 taccggtgac gttcgcgtag gatcgtttcg tggtcgccgc ttcgactccg ttgtcagtgc
     8761 ccgcgcttgg cgccgctgat ttcgcgtcac tgcggcgcgg ttccacactg gcgagggcgg
     8821 tcgattcgag aatcatggta gtttttc
//
LOCUS       DASQKS010000600         7467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4131818, whole genome shotgun sequence.
ACCESSION   DASQKS010000600 DASQKS010000000
VERSION     DASQKS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4131818"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VE170_15380"
     CDS             complement(<1..424)
                     /locus_tag="VE170_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018147463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16879.1"
                     /translation="MSATILELHNIEKHYTNGDTTVRALDGVSLRIRRGEFVAIMGQS
                     GSGKSTLMNIIGCLDHPSAGSYRVLGREAANLDADELASLRRETFGFIFQRYNLLATA
                     TAQENVAIPSVYAGLAKHKRKTHANELLKRLGLSERTDH"
     gene            complement(421..1638)
                     /locus_tag="VE170_15385"
     CDS             complement(421..1638)
                     /locus_tag="VE170_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ16880.1"
                     /translation="MKWPNKTVRNKILAGITVAGLTAGGWYWYTSSAQSSQGEVVQPI
                     TVRRGNLEEVVTSQGKLEAKQYVDVGTQVSGQLKAIHVDIGDTVTKGQLLAEIDPRVY
                     QAQVEAGEAKLNSLRAQLNQQKAEAVLAEQNLKRNQNLIAVNAVSQQALQETESQFAV
                     AKAQVDSIAAQMQETESNLKASRANLSYTKIYAPMAGTVTTLPTKEGQTLNANQTAPT
                     VLQIASLDVMTVRAQVAEADVSRIKVNMPVYFTTLGNSERRWPGTVRQVLPTPQIVND
                     VVLYDVLIDVKNEGRQLMTSMTTQVFFIFGKAENALIVPAEILTRRAAQQDNDKGKAY
                     RIAVITEKGTEQRVVHVGLQSRTQAEVVDGLNEGERIAVNRPAGAANRTASQANNPQR
                     GAGNQRLNRGPQL"
     gene            complement(1775..2206)
                     /locus_tag="VE170_15390"
     CDS             complement(1775..2206)
                     /locus_tag="VE170_15390"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HYJ16881.1"
                     /translation="MSQKTKLAFIVSIVLNVLLVGVLLGQSPRRFDPGAMREQRMEQV
                     IKDLPAESQTRLRERFKQLRATAEPMFREMRQVQDESARLLAVEPFDEAAFDGQQAKI
                     NQMRADMTKKLSEVVKNAVKGLTPEERQRFADLLRRPPPPA"
     gene            complement(2203..2517)
                     /locus_tag="VE170_15395"
     CDS             complement(2203..2517)
                     /locus_tag="VE170_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16882.1"
                     /translation="MSEYQDEKLESLLRSRHAETVPPDLAGRIILQAKSVPQLQNISL
                     WQGVRQLFAEFHLPKPGYVLASALVLGMVLGFSTAPENSQLSDASNVTVQSFIAGDEE
                     LL"
     gene            complement(2525..3079)
                     /locus_tag="VE170_15400"
     CDS             complement(2525..3079)
                     /locus_tag="VE170_15400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ16883.1"
                     /translation="MAVAWPDKEDNELLALVQDGSHPAFAELVQRHTERFYRLAFRYL
                     QNKQAAEDVVQDAFLKLWEDPKKWQADKNSKFTTWFYRVVVNLCLDWQKRKRPVALNE
                     EMPLIDDRESADQAMLRREEQQMLEQAIAALPERQRTAINLWFDEGLSNQDAADAMGV
                     NLKALQSLIMRAKTTLKERMKNYL"
     gene            complement(3456..4046)
                     /locus_tag="VE170_15405"
     CDS             complement(3456..4046)
                     /locus_tag="VE170_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16884.1"
                     /translation="MNPAHSPHCRACKERIGELLSAGFGDCRINHRFPWSSYPEAYPN
                     TPVGNHLEQIRRALGELRGHRDFIKSAQMPPCDYFVTDPPFIVEYDEDQHFSRARLVT
                     LSLYPKDRDWGFSLPRWLDLCAKIDAVDDTPIDRDERRAWYDTLRDLVPLAHGFNPTV
                     RLYAGEFDWCKLDTGASTVRNHLKELLGDRLRDGMP"
     gene            4267..6507
                     /locus_tag="VE170_15410"
     CDS             4267..6507
                     /locus_tag="VE170_15410"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ16885.1"
                     /translation="MANLSSIIGVSVGRVEGADKVSGSAIYGADVHFADALWAKILRS
                     PHPHARITSIDTSKAWKVPGVKAIVTGKDHPEHYQGKSIRDIPVLCWDKVRYIGDKVA
                     AVAAESADAAEEAVNLIDVEYEELPAVFDVLEAMKPDAPVLHDNAPAYDGAPAEIMAP
                     AGGNVVNKLTFGKGDIDKGFAEADLVLEHTFRMPIHHQGYLEPQAFLVKIENDGTVNA
                     WASTKGPFGSRGQFAKAVGIAPKQIHLQAVHVGADFGGKSGAGELPICYFLAQQAKRP
                     VKIVLTHSEELTAMNPDHYTVVKVKTGVKRNGRMTARYLQAIHGTGAYAGMKPGRASI
                     GGAGSATPYKIDNSYMEALQVYTNTVPCGFWRAPGAIQAAFASESHMDLIAKELKMDP
                     AKFRMMNLVGEGEENSLGKSWSGVKAKETLKAALDAADWKSPKRANVGRGLAMYERGT
                     GAGKVWVNLTAELDGSLTVFTVAGDQGTGLQTVLCQTVASEMQVPYDQVRCRIGNTAD
                     VTYSVDVGFGGSRSTNINSAAAYQASAELRKKLNAQAAILLSCAEDQVVYEAGQFSSK
                     GSRRKLLTLSEVVNSTDAPVTVSVETEVPRKGSSTSFIAQVAEVEVDRETGRIKLTKF
                     FSAHDVGTIINPLGHQGQIDGEAIMAIGSGLMEELTHDQGKVTTTNLGEYKIPNILDI
                     PKFTTVLVKGKNGPGPYEAKGIGEHANVTPPAAIANAVQDACGVRIFDTPVTAEKVYA
                     ALRAQG"
     gene            6571..>7467
                     /locus_tag="VE170_15415"
     CDS             6571..>7467
                     /locus_tag="VE170_15415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16886.1"
                     /translation="MNDKSRNRRLLSAVLFFFIGGWPLAAPSQDKIKIAYSSTDTLNQ
                     IWTIPQEIGLYKKNGLDVELVYIGSTTVGVSAIMAQDIQVGNAAGSGVANANVRGADT
                     VSVGCTINVLAYELVVLDSVKTAEDLKGKSIGISRFGSVSDVAARELLKGLGLRPMDD
                     VKILQVGGAAERAAGFSRGVIAGFPSPPGNVHLIPGGLPHRILANLSDLKKPYPLPFI
                     CAVTTKSYLAKSRSVVRRVMMSLIEASHYFKTNKEGAQKVVAKYLRGANKAYLDSSYE
                     STAKILERVPYTSREGMKIQLDE"
BASE COUNT         1574 a         2055 c         2230 g         1608 t
ORIGIN      
        1 ggtgatcggt gcgctcgctc agtcccagcc gctttaacag ctcgttcgca tgagtcttgc
       61 gtttgtgttt ggccaggccg gcgtacaccg aaggtattgc gacattttcc tgggccgtcg
      121 ccgtggcgag aagattatag cgttgaaaaa tgaagccgaa agtttcgcgc cgcagcgacg
      181 ccagctcgtc ggcgtcaaga ttcgccgctt cgcggccgag cacccgataa ctgcccgcgc
      241 tcgggtgatc gagacagccg atgatattca tcaaggtcga cttgccggag ccggactggc
      301 ccatgatcgc gacaaattcg ccccggcgga ttcgcagcga gacgccgtcg agggcgcgca
      361 cggtggtatc gccgttggtg taatgtttct cgatgttatg cagttctagg atagtcgcgc
      421 tcataactgt ggccctcggt tgaggcgctg attaccggcg ccgcgttgcg gattatttgc
      481 ctgactggcc gtccggttgg ctgcgccggc gggtcgattc actgcgatcc gttcgccttc
      541 gttgagtccg tcgacgactt ccgcctgggt gcggctttgc aagccgacgt ggacgacccg
      601 ctgctccgtg cctttctcgg tgatcaccgc gatccgatag gccttgcctt tgtcgttgtc
      661 ctgctgcgcg gctcggcgcg tgagaatctc cgcgggtacg atcaaggcat tttccgcctt
      721 gccgaagata aagaatactt gggtggtcat gctggtcata agctgtcggc cttcgttctt
      781 gacgtcgatc aagacgtcgt agagcaccac gtcgttgacg atctgtggtg tcggcagcac
      841 ttgacgcacg gtgcccggcc agcggcgctc gctgttgccg agggtggtaa aatagaccgg
      901 catgttgact ttgatccggc tcacatcggc ttccgcgacc tgggcgcgca cggtcatgac
      961 atcgagactg gcaatctgca aaaccgtcgg cgcggtctga ttggcgttca atgtctgccc
     1021 ttctttggtc ggcaatgtcg ttaccgtgcc ggccatcggc gcgtaaattt ttgtataact
     1081 gagattggcc cggctcgctt tcaaattaga ctcggtttcc tgcatctgcg cggcgatcga
     1141 gtccacttga gctttggcca ctgcgaattg ggattccgtc tcctgcaaag cctgctggct
     1201 aacagcgttg acggcgatca gattttgatt gcgtttcaaa ttctgttcgg ccaaaaccgc
     1261 ctcggccttc tgttggttaa gctgggcgcg cagactgttt agcttcgctt ctcccgcttc
     1321 gacctgggcc tggtaaacgc gcgggtcgat ctcggcgagc aattgtcctt tagttacggt
     1381 gtcgccgata tcgacatgga ttgcctttag ctggccggaa acctgagtgc cgacgtcaac
     1441 gtactgcttg gcttcgagct tgccttgcga ggtgacgact tcttcaaggt taccgcggcg
     1501 gactgtgatc ggctgaacca cttcgccttg agagctttgc gccgagctgg tgtaccagta
     1561 ccagccgccg gcggtgagcc cggcgacggt aattccggcg agtattttgt tgcgaactgt
     1621 cttatttggc catttcatag ggttttttcc gtcctctagg aggtttgtta actgttgata
     1681 cgacctagcc gagggaagtc ttcggcccac gcaaaaaaaa acgccgggag taccggcgct
     1741 tttggaaata ggtgtttttg caggggaatt taatttaggc cggtggcggc gggcgacgca
     1801 acaggtcggc aaaacgttgc cgttcctcag gcgtcaggcc cttgacggcg ttcttgacca
     1861 cttcggatag cttctttgtc atatcggcgc gcatttggtt gatcttggct tgctgcccat
     1921 cgaaggcggc ttcgtcgaag ggctcgacgg ccagaagccg cgccgattca tcctgaacct
     1981 ggcgcatttc acggaacatc ggctcggcgg tggcgcgcag ctgcttgaat ctctcgcgca
     2041 agcgggtctg tgactctgcg ggcaggtctt tgattacctg ctccatgcgc tgctcgcgca
     2101 tggcgccggg gtcgaaacgg cgcggcgact gcccgagtag tacgccgacc aggagtacgt
     2161 tcaaaacaat cgacacgata aacgccagtt tagtcttctg gctcataaca actcctcgtc
     2221 gccagcgata aaactctgca cggtcacgtt actagcgtcg ctcaactggc tattttccgg
     2281 ggccgtgctg aagcccagca ccattcccaa aaccagcgcg ctggcgagca cataccccgg
     2341 tttcgggaga tgaaattcgg cgaacaattg gcgcacgccc tgccacagcg aaatattttg
     2401 cagctgcggc acgctcttgg cctgcagaat gatccgcccg gccagatccg ggggcaccgt
     2461 ttccgcgtgc cgtgagcgca acagcgattc taatttttcg tcctgatatt cgctcataaa
     2521 atcctcacag ataatttttc atacgttctt tcaaggtggt cttggcgcgc atgatcaggg
     2581 actgcaaagc tttcaaattc acgcccatgg cgtcggcggc atcctgattg ctcaagccct
     2641 cgtcgaacca gagattgatg gcggtacgct ggcgctccgg cagggcggcg atcgcctgct
     2701 ccaacatttg ctgttcttcg cggcgtaaca tggcttgatc ggcgctctcg cggtcgtcga
     2761 tcaggggcat ttcttcgttc agcgccaccg gccgttttcg cttctgccaa tcgaggcata
     2821 gattcaccac gacccgataa aaccaggtgg taaacttcga gttcttgtcg gcctgccatt
     2881 ttttcggatc ttcccacaac tttagaaacg cgtcctgaac cacatcttcg gctgcctgtt
     2941 tattttgcag gtaacgaaat gccaggcgat aaaatcgctc ggtgtggcgc tgcaccagct
     3001 cggcaaaagc tggatggctg ccgtcctgaa ccaacgcgag cagttcattg tcttctttat
     3061 ccggccaagc aaccgccata gtctcgtttg ggcaaccttg ggagtggagt taaatctaaa
     3121 catttcccgc aacttgatgc gggcaccatc aaatggggaa tccaaagggg gcgagcatcc
     3181 cctttggtcg cgtgcggggt taataccccg tacgcgaaat gccttgttta atgtttcgat
     3241 catacattca tccgtgccag gagccaaagt cctcgatgca atgctccgtt aacccataat
     3301 acgaatgaag ataagaaagt cttcgccgac ggcggcggat ttgaattatt aaacgaattc
     3361 cccggatttc gttagcgaat ttaccgcgtt ccaaatggaa cgacggttcc gcggcagctc
     3421 aacaagcgcg cagacgatct ggtctatctt aggcgctagg gcatgccatc ccgcagccgg
     3481 tcaccgagca actctttcaa atgattgcgc accgtggatg caccggtgtc gagcttacac
     3541 cagtcaaatt ccccggcata gagtcgcacg gtgggattga agccgtgagc gagcggcacc
     3601 agatcgcgca acgtgtcgta ccaggcgcgg cgctcgtcgc gatcgatggg ggtatcgtcc
     3661 accgcgtcga tcttggcgca gagatccagc cagcgcggca gtgaaaatcc ccagtcgcga
     3721 tcctttggat agagcgacaa ggtcaccagc cgggcccgcg aaaagtgctg gtcctcgtca
     3781 tactcaacaa taaaaggcgg gtcggtaacg aagtagtcgc agggcggcat ctgcgcgctc
     3841 ttgatgaagt cgcgatggcc gcgcagttcg cccagggcgc ggcggatttg ctccaggtga
     3901 tttccgaccg gcgtgtttgg gtaggcttcc ggataggacg accaagggaa ccggtgattg
     3961 atccggcagt cgccgaagcc ggcgctcaat aactcgccga tacgctcttt acaggcgcgg
     4021 cagtgcggcg agtgggcagg attcacggtt aaatattacg caatcgttgc taggcgcttt
     4081 ggaacaagat agcaaactga acccaaagcg aaaatgatcg cggcggtgcc aggttgatgt
     4141 aatcacaaat cgctgcattg atgcaaaatt tcttgcctgc gtcagcgcgg gcctgattct
     4201 gttgcccgcc gggagcgatt aacgctatat tccgcacaag atcggtgatg gcgaggagaa
     4261 tttaacatgg caaatctgag ttcgatcatt ggcgtttcgg ttgggcgggt cgagggcgcg
     4321 gacaaagtta gcggctcggc gatttatgga gcggatgtac attttgccga tgccctatgg
     4381 gcaaagatcc tgcgcagtcc tcatcctcac gcgcgtatca cgagcatcga tacttcaaag
     4441 gcatggaaag taccgggcgt gaaggcgatc gttaccggca aagatcaccc ggagcactat
     4501 cagggcaaga gcattcgcga tattccagtc ttgtgctggg acaaagtgcg ctacatcggc
     4561 gacaaggtcg cggcggtggc agcggagagc gccgatgccg cagaggaggc ggtaaatcta
     4621 atcgacgtcg aatatgaaga gttgccggcg gtgttcgatg tgctcgaggc gatgaagccg
     4681 gacgctccgg tgctgcacga caacgcgcct gcttacgatg gtgcgccggc agagatcatg
     4741 gcgccggcgg gcggcaacgt ggttaacaaa ttaacctttg gcaagggtga tatcgacaaa
     4801 ggcttcgccg aagccgatct ggttttggag catacctttc gcatgccgat ccatcaccaa
     4861 ggctatctgg aaccgcaggc gtttctggtg aaaatcgaaa acgacggcac ggtcaacgcc
     4921 tgggcgtcga ccaagggtcc gttcggctcg cgcggccagt tcgccaaagc ggtgggtatt
     4981 gcgcccaaac agattcatct gcaagcggtc cacgtcggcg ctgacttcgg cggcaagagc
     5041 ggcgcgggcg agttgccgat ctgttatttt ctcgcccagc aggccaagcg gccggtgaag
     5101 atcgtgctca cccacagcga agaattgacg gctatgaatc cagatcacta cacggtggtg
     5161 aaagtgaaaa ccggcgtcaa gcgcaatggc cgaatgaccg cgcgatacct gcaagcgatc
     5221 catggcaccg gcgcctatgc cggcatgaaa ccgggacgcg caagcatcgg cggcgccggc
     5281 tcggcgacgc cgtacaagat cgataacagc tacatggaag cgttgcaggt ttacaccaat
     5341 accgtgcctt gcggtttctg gcgcgcgccg ggggcgatac aagcggcgtt tgcctccgag
     5401 tcgcatatgg atctgatcgc caaggaattg aaaatggacc cggccaaatt tcgcatgatg
     5461 aatctggtcg gcgaaggtga agagaattca ctgggcaaat cttggagcgg tgtaaaagct
     5521 aaggaaactt tgaaagctgc gcttgacgcc gccgactgga agtcgcccaa gcgtgccaac
     5581 gtcgggcgcg gcttggcgat gtacgagcgc ggcaccggcg ccggcaaagt gtgggtcaat
     5641 ctaaccgctg aacttgacgg cagcttgacg gtgttcaccg tcgccggcga tcaggggacg
     5701 gggttgcaaa cggtactctg ccagacagtc gcgtcggaga tgcaagtgcc gtacgatcaa
     5761 gtgcgctgcc gcatcggcaa cacggcggac gttacctaca gcgtcgatgt cggtttcggc
     5821 ggcagccgtt cgaccaatat taacagcgcg gcggcctatc aagcatcggc cgaattgcgc
     5881 aaaaaactca acgcgcaagc ggcgattctg ctcagctgcg cggaagatca ggtggtttac
     5941 gaggccggcc agttttccag caagggtagt agacgcaagt tgttgacatt gagtgaggtg
     6001 gtgaattcga cggacgcgcc ggtaaccgtc agtgtggaaa ccgaggtgcc gcgcaagggt
     6061 tcgtcgactt cgttcatcgc tcaggtggct gaggtcgagg tcgatcgcga aaccggccgg
     6121 ataaagctca ccaaattttt cagcgcccac gatgtcggca cgatcatcaa tccgctcggc
     6181 catcagggac agatcgacgg cgaggcgatc atggcgatcg gcagcggcct gatggaagag
     6241 ctcacccacg accaaggcaa ggtgacaacg accaacttgg gcgagtacaa aattcccaat
     6301 attctcgata tcccaaaatt caccacggtc ctagtaaaag gcaagaacgg ccccggcccc
     6361 tatgaagcca aaggtatcgg cgaacacgcc aacgttaccc cgccggcggc gatcgctaac
     6421 gcggtgcagg atgcgtgcgg cgtgcggata ttcgacacgc cagtgacggc ggaaaaggtc
     6481 tatgcggcgc tgcgggcgca ggggtgagaa atgggtgagc gcaagaatgt cgctgcaccg
     6541 ttcggcgtgg ctgttattta aggaggactt atgaacgaca agagtcgcaa ccgccgcttg
     6601 cttagtgccg ttttattctt tttcattggc ggctggccac tggcggcgcc atcccaggac
     6661 aaaattaaga tcgcctacag ttccaccgat acgctcaatc aaatctggac catcccgcag
     6721 gagatcggac tgtacaaaaa gaatggcctc gacgtggagt tggtttatat cggcagcacc
     6781 accgtgggtg tctcagcgat catggcccaa gacattcaag tcggcaatgc cgccggaagc
     6841 ggcgtcgcca atgccaacgt gcgcggcgcg gacacggtga gcgtcggctg cacgatcaat
     6901 gtgttggcct acgagctggt ggtgctcgac agcgtcaaaa ccgcggaaga tttgaaaggt
     6961 aagtcgatcg gcatcagccg ctttggcagc gtctctgacg tagctgctcg ggagttgtta
     7021 aaaggcttag ggttgcggcc catggacgac gtgaagatcc tgcaagttgg cggagcggcg
     7081 gaacgcgctg ccggattcag ccgcggcgtg atcgcgggat ttccctcgcc gcccggcaac
     7141 gttcatctga ttccgggcgg cttgccgcat cgtattctgg caaatctgtc ggatctcaaa
     7201 aaaccttatc cgttgccgtt catttgcgcg gtgacgacga aaagctacct ggcgaaaagc
     7261 cgttcggtgg tgcgccgagt tatgatgtcg ctgatcgaag catcacacta tttcaagacc
     7321 aataaagagg gcgcgcaaaa ggtcgttgcc aagtatctgc gcggcgccaa caaagcgtat
     7381 ttagacagtt cctacgagtc cacggcaaaa atcctcgagc gggtcccgta cacgtcccgt
     7441 gaaggaatga aaatccagct cgacgaa
//
LOCUS       DASQKS010000601         7348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4133518, whole genome shotgun sequence.
ACCESSION   DASQKS010000601 DASQKS010000000
VERSION     DASQKS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133518"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VE170_15420"
     CDS             complement(<1..197)
                     /locus_tag="VE170_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16887.1"
                     /translation="MLKQVHWSRKRKSAPTQSSKLLFEFRQLGKSSSSLHGFIAFDWF
                     WYRPPPLCDLALRDSCLDSPS"
     gene            274..3393
                     /locus_tag="VE170_15425"
     CDS             274..3393
                     /locus_tag="VE170_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HYJ16888.1"
                     /translation="MKILLKIAAGIGLTAVLFFIVCALAFYHLVRVGEFRNFLIDEIE
                     KNTAFKVGLSAADIEIGWITGIAFRDVVIATPDSATPEIAAERITARVALLPLLQRQV
                     IFYEIRMQRPTVALAGDAEGRIPLLDKLINLPILKRPGAQFGLDLQTIKIHDGAIDYF
                     DGRKRAGLGAWHLRQTDVTVERVRGQRLRELMKDTLVRPSAASTGAALRFEINSHVEK
                     DHAPMQLKASGRLVFPLEVLEFHQAYWKADVVVLDVPAALIKAQVGAHSPIKSVSGRI
                     AQRLHLEGYPEKQLRLKGDIEFRELSIDAPELLFAPLTDADGRTSFEAEWSRGRLAIK
                     QADLRIDDLKFALAGTIGALNSADPHLSLQLSPLSAPLTTLRRYLPLRIIESPQWEKS
                     LSSVQSGQVALKSAGVNGTLGELRRLMQTGIGKQLWFDAELRDISGSLAARGTLPLRG
                     VQGRVSLQEGRLTVRDLKGTYGDSQLHDVDASYDLTPSGRGALELSARGDFNLAELRS
                     QLRTEFVSERAAKIVAAIHELDGRSRVDLTVKRGANEPLQFAGKVALDNATFRYDDYA
                     LSEIKGEIAFTPRVIRGEKIRAQMSGAPLQLQFALTEYGADDGTFDLGVESTGVPAGV
                     IARLLLDQGSPNDPGVVRGSVRYSGALANKNMRKFTGDLDLINIQLLLKPLLQPLREL
                     SGKIKIDESGIDFQNLKTLLVGVPASASGRWRYAEKPQLLFDFSAPYLDVTYLISQID
                     PIVGEFYTNLQAEGKIALTKGRIKNFEFGDMKTDATIDRRVWRLTNLNARSAGGTIVG
                     VTTIFDRPDILGIVAQPRIQGVPVESFLRWFNISNTEMTGRVNLTGNLETTGNNDAER
                     KQNLNGGFNLRIEDGTINRMRTLVQILNLLDLSRWFTLQLPDLTKQGIRFRAITADFK
                     VTKGIYVTENLLVDSNDLRMSGAGKIDVPNDEIDLAVAVRPFAGIESAFNQVPILGRG
                     VAAIMNSFLVASFSIKGRIEEPAITPMPLGTLSEMFWGVLGIPKSILTLGDSEKKPEL
                     KEPPQAPAK"
     gene            3390..3680
                     /locus_tag="VE170_15430"
     CDS             3390..3680
                     /locus_tag="VE170_15430"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HYJ16889.1"
                     /translation="MTSKLTLYSRKDCCLCDAMKAVIDKVAANLPLEIDEIDIDGSSE
                     TREKFADQVPLLFIDGRKAFKYRVTAKQLAKKLGRKPPKLFTMIAGFLGKGE"
     gene            3677..4681
                     /locus_tag="VE170_15435"
     CDS             3677..4681
                     /locus_tag="VE170_15435"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16890.1"
                     /translation="MMKIILRQCFLMLLIIADGRLLSAADRLIGVQSARVMSQSMPWI
                     AEETGIFRKYNLEFPLVYIGTSPLATAAMLGGDAQLMIDGGVGLVRTVVGGNSDLVFI
                     AGIKNHLTQSILAKPEFKRLEDLRGKKIGVTRIGATTHYFALQAFKRRGMEAGRDYAM
                     VQTGGAPEMLAALLSGAIDAGTMSAPWDARAVSQGYQYVVYGPDLRIAHVAVSFITRR
                     SFIAQNSPLLGRFMRAMAEAAKILHSDKEIVFKVLSKYLRIEDRKVLDQAYNAEIKSL
                     EPRMELRLEAVQAILDDITPTDPRAKKYKPQDLYDRRYLDEMEKSGFMDKLWGKAAAK
                     "
     gene            complement(5407..7242)
                     /gene="typA"
                     /locus_tag="VE170_15440"
     CDS             complement(5407..7242)
                     /gene="typA"
                     /locus_tag="VE170_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYJ16891.1"
                     /translation="MTQAQLTRRDDLRNIAIIAHVDHGKTTLVDALLHQSGIFRANEH
                     VAERIMDSMDLERERGITIMAKNTSIHYGNAKINIVDTPGHADFGGEVERTLAMVDGV
                     MLLVDASEGPLPQTRFVLKKALEAKLTPIVCINKIDRPDARIAVVLDEIYDLFIDLDA
                     SEDQLDFPVVYTNARAGTATLDLKQPGENLKPMFELIVNTLPGPSYDPAMPTQFQANN
                     LDYNDYVGRLAVGRVKNGTLAPGQYMLCRVGGKQEVVKITQVYGWQGLKRVEVTSVQA
                     GDIAAIAGIEDIGIGDTITDREKPQALPPLRIDEPTIAMMFCANNSPWSGREGEHVTS
                     RKLRERLFFEARRNVSLRVEETAQPDAFQVTGRGEFALAIVIETMRREGYEMMVSKPT
                     VITREINGGINEPMELLVVDIPEDFIGVVSQLLAMRKGKMTKMIHAGTSRVRLEFAVP
                     SRGLIGFRSRFLTDTRGTGIMNALFNGWEPWHGMIQSRTNAAMVADREGSAPPYAIYH
                     LQERGVMFIGPGTKVYEGMVVGEYSRENDLNVNICKEKKLTNMRASGKDENIIISPHR
                     EMGLEAALEWISDDELVEVTPQSIRLRKKILKQADRPRRRHEEED"
BASE COUNT         1547 a         2080 c         2152 g         1569 t
ORIGIN      
        1 caactagggc tgtcaaggca ggaatcgcgc agggcaagat cgcacagcgg cggtggacga
       61 taccaaaacc aatcaaaagc aataaagccg tgcaacgatg agctggattt gcccagttgc
      121 cggaactcaa atagtaactt ggaggactgc gtgggcgctg atttacgctt gcgactccaa
      181 tgaacttgct ttagcattgc gatggtttag cgtgccgcgt tccttcccag gagagcaacc
      241 gccctaagct ttgatgcggg tcttcgttta ctcatgaaaa tactcctcaa gatcgccgcg
      301 ggcatcggcc ttaccgcggt gctttttttc attgtttgcg ccctggcatt ttaccacctg
      361 gttcgagtcg gcgaattccg caacttcctg atcgacgaga ttgaaaaaaa taccgcgttc
      421 aaggtaggac tcagcgcagc ggacattgaa atcggctgga tcacgggaat agcttttcgc
      481 gacgtcgtca tcgcaacgcc ggatagcgct accccggaaa tcgccgctga gcggatcaca
      541 gcgcgagttg cactgctgcc gttgctgcaa cgtcaagtga ttttttatga gattcgcatg
      601 cagcggccca ccgttgccct agccggcgac gccgaggggc gcattccgtt gctcgacaag
      661 ttgattaatt tgccgatact caaacgcccc ggtgcgcagt tcggtctcga tcttcaaact
      721 atcaagatcc acgacggcgc catcgattat ttcgatgggc gtaagcgtgc cggcctgggc
      781 gcatggcatc tacgccagac cgatgtcacg gtcgagcgcg tgcgcggcca gcggctgcgc
      841 gagttgatga aggatacgct ggtgcgcccg tcggcggcat cgaccggcgc ggcgctgcgt
      901 ttcgaaatta acagccatgt agaaaaagac catgcgccga tgcagctcaa ggcttccggg
      961 cggttggtgt ttcccctgga ggttctggag tttcaccaag cttactggaa agccgatgtc
     1021 gttgtactcg atgtccccgc ggcattgatc aaagcgcagg tcggcgccca ttcgccgatc
     1081 aagtcggtga gcgggcgcat cgcccagcgg cttcatcttg aaggctatcc cgaaaaacag
     1141 ctgcggctca agggcgatat cgaattcaga gagctctcga ttgacgcgcc cgaactgttg
     1201 ttcgcaccgc ttaccgacgc ggatggccgg accagcttcg aggcggagtg gagccgggga
     1261 agattggcga tcaaacaggc cgatttgcgt atagacgatc tcaagttcgc cctggccggt
     1321 acgatcggtg cactcaacag cgccgatcct catctgtcgt tgcaactctc gccgctgtcg
     1381 gcgccgctga cgacgctgcg tcgctatttg ccattgagaa tcatcgaatc gccacaatgg
     1441 gaaaaaagtc tgagttcggt ccagagcggg caagtggcgc tcaagagcgc cggcgttaac
     1501 ggtacactcg gcgaactgcg ccggctgatg cagaccggta tcggcaaaca gctctggttc
     1561 gacgcggaac tgcgcgacat ctcgggcagt ctcgccgccc gcggcacgct gccgctacgc
     1621 ggcgttcaag ggcgggtgag tttgcaagag ggccgcttaa cggtgcgcga tctcaagggc
     1681 acctacggcg attcccaatt gcatgacgtc gacgccagct acgatttgac gccgagcggt
     1741 cgcggcgcgc tcgagctctc ggcgcgcggt gatttcaatc tggccgagtt gcgcagtcag
     1801 ctgagaaccg agtttgtttc cgagcgcgcc gccaagatcg tcgctgcaat tcatgaactc
     1861 gatggccgca gccgagtcga tttgactgtg aaacgcggcg ccaacgagcc gctccaattc
     1921 gccggcaaag tcgctctgga caacgccacg ttccgctacg acgactatgc cttgagcgaa
     1981 attaaggggg agatcgcttt tacgcccaga gtgatcaggg gcgaaaagat ccgcgcgcaa
     2041 atgagcggcg cgccgctgca actccaattc gcgctcacag aatacggcgc ggacgacggc
     2101 acttttgacc tgggtgtcga atccaccggc gtgcctgccg gcgtgatcgc gcgcttgctg
     2161 ctcgatcagg gatcgcccaa cgatcccggc gttgtccgtg gctcggtgcg ctacagcggc
     2221 gcgttggcga acaaaaacat gcgcaaattc accggcgact tggatttgat caacatccag
     2281 ctgttgttaa aaccgctctt gcagccgttg cgcgagctca gcggcaagat caagatcgac
     2341 gagtcgggta tcgattttca aaatctcaag acgttgctcg tcggggttcc ggcaagcgcc
     2401 agcggtcgct ggcgctacgc ggagaagcca caacttctat tcgattttag cgcgccctat
     2461 ttagatgtga cctacttgat ttcacagatc gacccgatcg tcggcgagtt ctacaccaac
     2521 ctgcaagccg aaggtaaaat cgccctgact aaagggcgca ttaagaactt cgagtttggc
     2581 gacatgaaaa ccgatgcgac catcgaccgg cgggtctggc gattgaccaa tcttaacgcg
     2641 cgctccgccg gcggtaccat cgtcggcgtg acgacgattt tcgaccggcc ggatatcctg
     2701 ggaattgtcg cacaaccgag aatccaaggg gtgccggtgg agtcgtttct gcgctggttc
     2761 aatatctcca acactgaaat gacggggcgt gtgaatctta ccggcaatct ggagacgacc
     2821 ggcaacaacg acgccgagcg gaagcaaaac ttaaacggcg ggttcaacct gcgcatcgag
     2881 gacgggacaa tcaaccggat gcgcaccctc gtgcaaatcc tcaatctgct cgatttgtcg
     2941 cgttggttta ccttgcagct gcccgatctc accaaacagg ggattcgttt ccgcgccatc
     3001 accgccgatt tcaaagtgac caaaggtatt tacgtgacgg aaaatttgct cgtcgacagc
     3061 aacgacctgc gcatgagcgg cgccggcaag atcgatgtgc cgaacgatga gatcgacctg
     3121 gcggttgccg tgcggccctt cgccggcatc gaatcggcgt tcaatcaagt gcccattttg
     3181 ggccgcggcg ttgccgccat catgaattct ttcctggtcg ccagttttag catcaagggc
     3241 cggatcgaag agccggcgat cacgccgatg ccgctaggaa ctctatccga aatgttttgg
     3301 ggcgttctcg gcattcccaa aagcatcctg acgctgggtg acagcgagaa aaaaccagaa
     3361 cttaaagaac ctccccaggc accggccaaa tgacgtccaa attgacctta tattcgcgca
     3421 aggactgttg cctgtgcgac gccatgaagg cggttattga taaagttgcg gcaaatctgc
     3481 cgctcgaaat cgacgagatc gacatcgatg gctcgagtga gacgcgcgaa aaattcgccg
     3541 atcaagtgcc gctgctgttc atcgacggac gcaaggcttt caaataccgg gtgactgcca
     3601 aacagctggc aaaaaagctc ggcagaaaac cgccaaaact cttcaccatg atcgctggtt
     3661 tcttgggaaa gggggaatga tgaaaatcat cttgcggcaa tgtttcctga tgctgttaat
     3721 catcgctgac ggccgtctgc tgtcggcggc ggatcgtttg atcggggtcc agtcggcgcg
     3781 ggtgatgtcg cagtcgatgc cgtggatcgc cgaggaaacc ggcatttttc gcaagtacaa
     3841 tttggagttt ccgctggtct acatcggcac ctcgccgttg gcaacggcgg caatgttggg
     3901 cggcgacgcg cagttgatga tcgacggcgg cgtcggactg gtgcgcaccg tggtaggcgg
     3961 caacagcgat ctggtattta ttgccggtat caagaaccat ttgactcaaa gcattctcgc
     4021 caagccggag tttaaacggc tggaagacct gcgcggcaag aaaatcggcg tcacgcgcat
     4081 cggtgcgacc acccactatt tcgcgctcca agcgttcaag cggcgtggca tggaagcggg
     4141 acgggactat gcgatggtac agaccggcgg cgccccggag atgttggcgg cactactctc
     4201 cggcgccatc gatgccggca ccatgtcggc gccctgggat gcccgcgctg tctcccaggg
     4261 ctaccagtat gttgtctacg gcccggatct gcgcatcgcg catgtcgccg tgtcgtttat
     4321 cacccgccgc tcatttatcg cccagaactc acccttgctt ggccgcttca tgcgcgccat
     4381 ggccgaggct gcgaagattt tgcacagcga caaagaaata gtcttcaaag tcttgagcaa
     4441 atacctgcgc atcgaagacc gcaaagtgct cgaccaagct tacaacgccg agatcaaatc
     4501 cctcgaaccg cgcatggaac tgcgactcga agcggtccag gcgatcctcg atgacatcac
     4561 gcccaccgac ccacgggcga aaaaatacaa accgcaggat ctgtacgacc gccgctactt
     4621 ggacgaaatg gagaaaagcg ggtttatgga caagctgtgg ggtaaagcgg cggcgaaatg
     4681 aaattagcaa gtacgggatt ccaatcggct aactctcgtt tcatgttgca acaattagcc
     4741 agcacgcgag gttacgctca agggtaacct gaaggcgatc taatcagttc tagtcccagc
     4801 ggtaacggaa aggtcactcg cgttaagcgt ctatcaagcc gcgcagaacc atcgcctgcg
     4861 agccactgcc aacgcgtaga cggggcagtc attcctgtcc ttcaccgtcg cacttgctgc
     4921 acttgccgct gccatggcaa gctccacaac ttcctggtcc cgaaccgttg tcgccggtcc
     4981 cgcgacatgt tccctgacat ctcccgctgc cttggcacaa gctacacttc accatttggt
     5041 actcctctcc gttcgaccga attaggcatt gcagcatcgc gcaactgtga atgcggcaaa
     5101 tctcagcaaa gccgaatcgg gtgaaatatt gtcccgttgc tcggagttgt caacaaaccg
     5161 tcggccatga aaagcctccg gttgatcggc ccgcgcggtt cttcaattcg cagtgtctct
     5221 ctgcccgaag actacctgca actctacgtc tcgaatagtg cgtggaatct tttgcttact
     5281 cgaattgttg aactgcagaa gataccggtg gttcagtgct tcagtccggc agtgtagtcc
     5341 cgtcagcggg gattcatact gtgacggcgg cgcggggcaa gaaacgtcga aggagtggaa
     5401 ataaggctaa tcttcttctt catggcggcg gcgcggccgg tcggcttgtt tgagaatctt
     5461 cttgcgcagt ctgattgact gtggcgtgac ttcaaccagc tcatcgtcgg agatccattc
     5521 caaagcggct tccaaaccca tttcacggtg cggcgagatg atgatgtttt catccttgcc
     5581 ggaggcgcgc atgttggtaa gttttttctc tttgcagatg ttgacgttca aatcgttttc
     5641 ccgcgaatac tcgccgacca ccatgccttc gtaaactttg gtgccgggcc cgatgaacat
     5701 gacgccgcgt tcctgcaaat gataaatcgc atagggcggg gccgagcctt cgcggtcggc
     5761 gaccatggcg gcgttggtgc gcgattgaat catgccatgc cagggttccc agccgttgaa
     5821 cagcgcgttc atgatgccgg tgccgcgggt gtcggtgaga aaacgcgagc gaaagccgat
     5881 caagccgcgc gacggcacgg cgaattccaa gcgcacgcgg ctggtgccgg cgtggatcat
     5941 cttggtcatc ttgcccttgc gcatggccag cagctgcgac accactccga taaaatcctc
     6001 gggaatatcg acgaccaaga gttccatcgg ctcattgatg ccgccattga tctcacgcgt
     6061 gatcacggtg ggcttggaga ccatcatctc atagccttcg cggcgcatgg tctcgatgac
     6121 gatggccagg gcgaattcgc cgcgtccggt gacttgaaag gcatccggtt gagcggtttc
     6181 ctcgacccgc agactgacgt tgcgccgggc ttcgaagaac aatcgttcgc gcagcttgcg
     6241 cgaggtgaca tgttcgcctt cgcggccgga ccagggtgaa ttgttggcgc aaaacatcat
     6301 ggcgatggtc ggttcgtcga tgcgcagcgg gggtagcgct tgaggttttt cccggtcggt
     6361 gatggtatcg ccgatgccga tgtcctcgat gccggcgatg gcggcgatgt cgccagcttg
     6421 caccgatgtg acttctaccc gtttgagccc ctgccagcca tagacctggg tgattttcac
     6481 cacttcttgc ttgccgccga cgcggcaaag catatattgt cccggcgcca gcgtgccgtt
     6541 cttcacccgg ccgacggcga gcctgccgac gtagtcgttg taatcgagat tgttggcttg
     6601 gaattgtgtc ggcatcgccg ggtcgtagct ggggcccggt agggtgttga cgatcagctc
     6661 gaacatcggt ttcaaatttt caccgggctg tttcaagtcg agcgtcgcgg tgccggcgcg
     6721 ggcgttggta tagacaaccg gaaaatcgag ctggtcttcg ctggcgtcca gatcgatgaa
     6781 caggtcatag atttcatcga gcaccacggc gatacgggcg tcgggccggt cgatcttatt
     6841 gatgcaaacg atcggcgtaa gtttggcttc cagggctttc ttcaagacga agcgggtctg
     6901 cggcagcggc ccttccgagg cgtcgaccag cagcatgacg ccgtcgacca tcgctagggt
     6961 gcgttccact tcgccgccga aatcggcatg gccgggcgta tcgacgatgt tgatcttggc
     7021 gttgccgtag tggatcgacg tgtttttcgc catgatcgta atgccgcgct cgcgctcgag
     7081 atccattgaa tccatgatgc gttcggcgac atgctcgttg gcgcggaaaa tgccgctctg
     7141 gtgcaacaag gcatcgacca gagtggtctt gccgtgatcg acgtgggcga tgatcgcgat
     7201 gttgcgtaaa tcgtcgcggc gggtgagctg agcttgagtc ataaccgatt agtatcgaag
     7261 ttggctccgc cgaagtcaag cgaacggcga aaatgtccgc aattctcgag cgttggcttt
     7321 cgttaccttg cgattaccgg ggctgtag
//
LOCUS       DASQKS010000602         5577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6235860, whole genome shotgun sequence.
ACCESSION   DASQKS010000602 DASQKS010000000
VERSION     DASQKS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6235860"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VE170_15445"
     CDS             complement(<1..552)
                     /locus_tag="VE170_15445"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HYJ16892.1"
                     /translation="MPIAKEGWPLILSAAALAVLALLLRWQMAGIALALLTLAIAAFF
                     RDPERDIPTGDGLVVSPADGRVVTVAPVQGDGLFADAVTRISIFLSPLDVHINRAPVA
                     GRVNDVRYQSGKFLPAFKEDASRVNEQNAVSLVDERERNWGVVQIAGAVARRIVCRAK
                     TGDMLGRGERFGLIMFGSRTDIYL"
     gene            complement(573..1712)
                     /gene="ilvC"
                     /locus_tag="VE170_15450"
     CDS             complement(573..1712)
                     /gene="ilvC"
                     /locus_tag="VE170_15450"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HYJ16893.1"
                     /translation="MYFRSPARSQLNLVWLQILVVDLKRVYRHSRKPKVQRRNHSMQV
                     YYDRDADLNVLKGKKVAVLGYGSQGHAHANNLRDSGVEVVVGLRRGSSSWAKAENAGL
                     KVCETADAVALADIVMVLLPDELQGDAYEKEIRPGLKSGNYLAFGHGFNIHFKRIVAP
                     AGVNVFMVAPKGPGHLVRSEYEKGRGVPCLLAVHQDPSGDTTKMGLAYASAIGGARAA
                     VIQTTFKDETETDLFGEQSVLCGGLCELIHAGYETLVDAGYPPEMAYFECVHEVKLIV
                     DLIYEGGMTNMRYSISNTAEYGDLTRGKRVIGPEVRQAMQKILKDIQSGQFADEWINE
                     FKAGSPHFTELRKEQANHPLEKVGAGLRSMMPWLGQNRLVDRSKN"
     gene            complement(1879..2400)
                     /gene="ilvN"
                     /locus_tag="VE170_15455"
     CDS             complement(1879..2400)
                     /gene="ilvN"
                     /locus_tag="VE170_15455"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HYJ16894.1"
                     /translation="MEHIISVLVENKFGVLARVAGLFSARGYNIESLSVAPTLDPTTS
                     MITIVTIGDDRIIEQIMKQLNKVVEVLSVIDHTETDFLDRETVLIKVHTKPEHRDEAI
                     RIASIFRAKIVDSSALTYTIEITGDVDKVEAMINMLKPLGIKDLIRTGRIAIARETTR
                     TASPRREPALLKP"
     gene            complement(2413..4194)
                     /gene="ilvB"
                     /locus_tag="VE170_15460"
     CDS             complement(2413..4194)
                     /gene="ilvB"
                     /locus_tag="VE170_15460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HYJ16895.1"
                     /translation="MKITGAEIFCESLRREGVKTVFGLPGGVVLKIFDVLCQRKDLQL
                     ILTRHEQGAAHMAEGYAKATGKAGVVLVTSGPGMTNIVTGLADAHMDSVPLVAFTGQV
                     STDLIGNDAFQEADNVGISRPCTKHNVLVKDVNDLAQAVKEAFYIATSGRPGPVLVDI
                     PKDVSTAKAEFKYPDKINLRGYRPFSGGDKSQIKQAAEAIMTAKKPILYVGGGAVFGD
                     AADEVRELAEITQIPVTMTLMGLGTFPGTHELSLGMLGMHGGYWSNMAMHQADLLIAV
                     GARFDDRVTGKLSEFCPDARVIHIDIDPTSIRKTFHAHIPIVGDVKSVLRQINVVLRS
                     LDGNPTAMKARRAPWLKQINAWKKEHPLVYKQDDKITKPQYVVEKLYELTKHEAIVAT
                     DVGQHQMFAAQYFKGKKPRSWLTSGGLGTMGFGFPAAIGAQVAFPNRQVVCITSEGSF
                     QMNLQELVVAAEHDLPVKIVLLNNGVHGMVRQWQDLFYESRYSASLLGNKPDFVKLAD
                     AYGIKGLRALRPGEVDGVLKEGLKHKGPVLMDIHTDPFENCYPMIPAGGAQHEMMLED
                     PPELIQSRKDGTVHKRKVDEGEGVLPA"
     gene            complement(4222..4440)
                     /locus_tag="VE170_15465"
     CDS             complement(4222..4440)
                     /locus_tag="VE170_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HYJ16896.1"
                     /translation="MEHSEESVIVSLLDKDPELKKYYDEHLALEKQLATFQHKGHLTA
                     DEEVEMKRIQKLKLVGKDKIMELLGKHR"
     gene            complement(4546..5274)
                     /gene="tsaB"
                     /locus_tag="VE170_15470"
     CDS             complement(4546..5274)
                     /gene="tsaB"
                     /locus_tag="VE170_15470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYJ16897.1"
                     /translation="MRILGVDTSTTSASVALIEDDQLIAEAGEGREHSIGLISARGNH
                     SETVLPMIQAVLDKSDCRLADLTAIAIAIGPGSFTGLRIGLALVKGLAYDGNLPVIGV
                     PSLEAQAERVRAFAGLICPLLDARKEEVYTAVFDRRDGILRRRFADRACAVTTLATLF
                     DPLPSAPAIAFIGAGAERYCHELLRLFAGRAQLIGETDAESAAHALARLALRRFADAP
                     GGDLGQLVPFYLSSFTGLGNTPLN"
     gene            complement(5271..>5577)
                     /locus_tag="VE170_15475"
     CDS             complement(5271..>5577)
                     /locus_tag="VE170_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYJ16898.1"
                     /translation="NIGGPILIAQMAGQQAQEGVGNFLHFLAVLSINLGVLNLLPVPV
                     LDGGHLLFFLVEAVIRKPVSVRVREMAQQVGICLLALLMVYAFYNDIVRFFEKQVGG"
BASE COUNT         1246 a         1622 c         1460 g         1249 t
ORIGIN      
        1 taaataaata tcggtgcgcg agccgaacat gatcaaaccg aagcgttcgc cacggcctaa
       61 catatcaccg gttttcgcgc ggcagacaat gcgcctggca accgcgccgg cgatttgcac
      121 cacgccccaa ttgcgctccc gttcgtcgac cagactcacc gcgttctgct cgttgacgcg
      181 ggaggcgtct tccttaaagg ccgggagaaa tttgccgctt tgatagcgca cgtcgttaac
      241 ccgcccggcc accggcgccc gattgatatg cacgtcgagg ggcgatagga agatgctgat
      301 acgggtcacg gcatcggcga acaggccatc gccctgcacc ggcgcgacag tgaccacccg
      361 gccgtccgcg ggggaaacca ccaagccgtc accggtgggg atgtcgcgct ccgggtcgcg
      421 aaaaaatgcc gcgatcgcca gcgttagcag cgccaatgcg ataccagcca tctgccagcg
      481 caacaacaac gccagcaccg ccagcgctgc tgccgacaga atcaatggcc aaccttcctt
      541 tgcgataggc atcgtgcgct cagagcaaca gcttaattct tacttcggtc gaccagacga
      601 ttctgaccca gccagggcat catcgagcgc aagccggcgc cgactttttc cagcggatga
      661 ttggcctgct cttttctgag ctcggtgaag tgcggcgatc ccgccttgaa ttcattgatc
      721 cactcgtcgg cgaattgacc ggattgaatg tcctttaaaa tcttctgcat cgcttggcgc
      781 acttccgggc cgattacacg cttgcctcgg gtcaagtcgc cgtactccgc ggtgttgctg
      841 atcgagtagc gcatgttcgt catgccgccc tcgtagatca gatcgacgat cagcttgact
      901 tcgtggacgc actcgaaata cgccatctcg ggcggatagc cggcgtcgac cagagtttca
      961 taaccggcat gaatcaactc gcagagacct ccgcacagta ccgattgttc gccgaagaga
     1021 tcggtctcgg tttcgtcctt gaaggtggtc tggatcacgg cggcacgggc accgccgatg
     1081 gcgctggcgt aggccaagcc catcttggtc gtatcgccgc tgggatcctg atgcaccgcc
     1141 aacaggcacg gcacgccgcg tcctttctcg tactcgctgc gtaccaggtg gccggggccc
     1201 ttgggggcga ccatgaatac gttgacaccc gccggcgcaa cgatcctctt gaaatgaata
     1261 ttaaacccat ggccgaaggc caagtagtta ccgctcttca atcccggccg gatttccttt
     1321 tcgtaagcgt caccctgcaa ctcatcgggc aacagaacca tgacgatatc cgccagcgct
     1381 accgcatcgg cagtttcgca gactttgagg ccggcgtttt ccgcctttgc ccaggagctg
     1441 ctgccgcggc gcaggccgac gacgacctcg acgcctgaat cgcgcaaatt gttggcatgc
     1501 gcatgtccct ggctgccgta accaagcacg gcgacctttt ttcccttcaa cacattgaga
     1561 tccgcatcgc ggtcataata aacttgcatg gaatgatttc tcctctgaac tttaggcttt
     1621 ctactatgcc gatagactcg ctttaagtca acgacaagta tctgtaacca aacgagattt
     1681 agttggctcc gggcgggcga cctgaagtaa atcggaaagt agtttgtcgt tccgaaaaga
     1741 tccgctaagt aaaaatatca aggcggagat catgcaccgg ccgtaaacgg acaccaagaa
     1801 agaaaaacac cgccggccgt gggtgctgag agtggctttt gttatcttga cctgcggggg
     1861 acagtcgaca gcgtcgtttt acggttttaa taaagccggt tcccggcgcg gcgatgcggt
     1921 tctggtggtc tcgcgcgcaa tcgcaatccg gccggtgcgg atcaagtctt taattcccag
     1981 cggcttgagc atgttgatca tcgcctcgac tttgtcgacg tcgccggtaa tttcaatcgt
     2041 gtaagtgagc gccgacgagt cgacgatttt ggcgcgaaag attgacgcaa tgcgaatcgc
     2101 ttcgtctcgg tgctccggct tggtgtggac tttgatcaac accgtctcgc ggtctaaaaa
     2161 gtcggtttcg gtatgatcga tgacactcaa cacctcgacg actttgttga gttgcttcat
     2221 gatctgttca atgattcgat catcaccgat ggtcacgatc gtgatcattg aagtcgtcgg
     2281 gtccagggtg ggcgccaccg acaaactctc gatattgtac cctcttgcgc taaaaagacc
     2341 tgcgactctg gctaggacgc cgaatttatt ttcgacaaga accgatatga tatgttccat
     2401 tcgctaacta tatcaggcag gaagaacacc ctcgccttca tccacctttc tcttatgtac
     2461 tgttccatcc ttacgcgatt gaatgagttc cggcgggtct tccagcatca tttcatgctg
     2521 cgctccgccg gcgggaatca tcggatagca attttcgaag ggatcggtat gaatgtccat
     2581 caacaccggc cccttgtgct ttaacccttc tttgagaaca ccgtcgactt cgccgggcct
     2641 cagcgcgcgc aaacctttga tgccataggc atcggcaagc ttcacgaaat ccggcttatt
     2701 tccgagcaaa ctcgccgaat agcggctctc gtaaaataag tcttgccact gacgcaccat
     2761 gccatgcacg ccgttgttga gcaaaacgat cttcaccggt aaatcatgtt ccgccgccac
     2821 caccagctcc tgcagattca tctgaaaact gccttcgctg gtgatacaaa ccacttggcg
     2881 gttggggaac gccacctgcg cgccgatggc ggcaggaaaa ccaaatccca tggtgcccaa
     2941 accacccgag gtaagccagc tgcgcggctt ctttcctttg aaatactgcg ccgcaaacat
     3001 ctggtgctgg ccgacatcag tagcgacgat ggcttcgtgc ttggtcagct catagagctt
     3061 ctcgacgaca tattgtggtt tagtaatctt atcgtcttgc ttgtaaacta gcggatgctc
     3121 ttttttccaa gcattgatct gcttgagcca tggcgcgcgc cgcgctttca tcgccgtcgg
     3181 gttaccatcc aacgaacgca acacaacgtt gatctgccgc agcaccgatt tgacatcgcc
     3241 gacgatcgga atatgggcat gaaacgtctt gcggatcgac gtcggatcga tgtcgatatg
     3301 aatcactcgc gcatcgggac aaaactccga gagtttgccg gtgacacggt cgtcgaaccg
     3361 agcgccaacg gcaatcaaga gatcggcctg gtgcatcgcc atattgctcc aatagccgcc
     3421 atgcatgccc aacatcccca acgaaagctc gtgggttcca ggaaaagtgc cgaggcccat
     3481 taatgtcatg gtcacgggaa tctgcgtgat ctcggcgagc tcgcggactt cgtcagcggc
     3541 atcgccaaac acggcgccgc caccgacata gaggatcggc ttcttagccg tcatgatagc
     3601 ttccgccgct tgcttgatct gcgatttatc gccgccgctg aatgggcgat agccgcgcaa
     3661 gttgatcttg tcaggatatt tgaattcggc cttggctgta ctgacatctt tggggatatc
     3721 gaccaaaacc ggccccggtc tgccgctggt cgcgatgtaa aacgcttcct tcaccgcttg
     3781 agcgagatcg ttcacgtctt tgactaacac gttatgctta gtgcaggggc ggctgatacc
     3841 gacattatcg gcttcttgga aggcatcgtt gccgattaaa tcggtggaaa cttgtccggt
     3901 aaacgcgacc aatggcaccg agtccatatg cgcgtcggcc aaaccggtaa caatattcgt
     3961 catgccgggc ccggaggtca ccaacaccac gccagctttg ccggttgctt tggcatagcc
     4021 ctcggccatg tgagctgcgc cctgctcatg gcgcgtcaag atcaactgca aatctttgcg
     4081 ctggcacagg acatcgaaaa tttttagtac gacgccgccg ggcagaccga aaacggtttt
     4141 cactccttca cggcgcaaag actcacaaaa aatttctgct ccggtaattt tcatagggct
     4201 ccttagctcc ggctgccgaa cctaacggtg tttgccgagc aactccataa ttttgtcttt
     4261 gccgacgagc ttaagttttt ggatgcgttt catttcgact tcttcatcag ccgtcaaatg
     4321 ccctttatgt tgaaatgtgg caagttgctt ctctagggct agatgctcat cgtagtattt
     4381 cttcagttct ggatccttat ccagcaggga gacaatgacc gattcttcac tatgttccat
     4441 aataacagcc tcctttggga aatcgtaatt ctgagcgggg tcaatctaac ttacggtctg
     4501 cggattgtca acgtagcttt ctcgttacta tttcaacggg ttaggtcaat tcagtggggt
     4561 atttccgagg cccgtaaaac tactcaaata gaacggtacc agctggccta aatcaccccc
     4621 gggagcgtcg gcaaagcgcc taagcgccaa ccgcgccagc gcgtgggccg cactttcggc
     4681 gtcggtttcg ccgatgagtt gagcgcgccc tgcgaacaag cgaagcagct catggcaata
     4741 acgttcggcg ccagcgccga taaaagcaat tgccggcgct gaaggcagcg gatcgaacag
     4801 cgttgcgagg gtggtgactg cacacgcccg atcggcaaat cggcgccgca ggatgccgtc
     4861 gcgccgatcg aatacggcgg tataaacttc ttccttgcgc gcgtcgagca atggacagat
     4921 aagcccggcg aacgctcgca cccgctcggc ttgcgcctcg agactcggca cgccgattac
     4981 cggcaagttg ccatcgtagg ccaatccttt gacgagagcg agaccaatgc gtaaaccggt
     5041 aaacgaaccc ggaccgatgg ctatggcaat ggcagttaga tcggcaagtc ggcaatcgga
     5101 tttgtcgagc accgcttgaa tcatcggcaa gacggtttct gagtggtttc cccgagcact
     5161 gattaatccg atggaatgtt cgcgcccctc gccggcttcg gcgatcagtt ggtcgtcttc
     5221 gattaaagca acactggcgc tggtagtgga agtgtcaacg cccagtatcc tcatccaccg
     5281 acctgcttct cgaaaaaccg cacgatgtca ttgtaaaaag cataaaccat caacaaggcc
     5341 aacaaacaga taccgacctg ctgggccatc tcccgcacac gcaccgaaac cggtttgcgg
     5401 ataaccgcct cgaccaggaa aaataacaag tggccaccat cgagcaccgg caccggcaag
     5461 agattaagca cgcctaaatt gatactgagc acggcgagga aatggagaaa gttgccaacc
     5521 ccttcttgcg cctgctgtcc ggccatctgg gcaatcagaa tcggaccgcc gatgttc
//
LOCUS       DASQKS010000603         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5378952, whole genome shotgun sequence.
ACCESSION   DASQKS010000603 DASQKS010000000
VERSION     DASQKS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5378952"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VE170_15480"
     CDS             <1..500
                     /locus_tag="VE170_15480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16899.1"
                     /translation="GITQEYAAVMLSVFAGLNLITHFVLGWIADRINKPKLLSLWMLL
                     PIAGIIILRTGDSLVSLWLFTIFFSTLDAAFPVTWATSGDFFGRKYFATIRGNMTFVY
                     MWGSALGPVIAGYLYDQTQSYGAILWAIVATLSVSVMLTSLLIKPWRDKVRHLSTASR
                     RSSLS"
     gene            594..3140
                     /locus_tag="VE170_15485"
     CDS             594..3140
                     /locus_tag="VE170_15485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HYJ16900.1"
                     /translation="MAEPRSHRLPTSVIPERYEIELTPDLLQWTFTGEVKIAVDVRQE
                     VREVVLNAAELVIQSVSLRSADGTPHHGNVQVDDANEQAVLSFPDLVAPGNYQLQIHF
                     TGVLNDKLHGFYRSVYKGVDGQDKPLASTQFESTDARRAFPCWDEPAFKAVYQVTLVI
                     DEKLTAISNARVIEETVLPGTGKKAVRFADSIKMSTYLVAFIVGDFEATEPMTADGVP
                     LRVLAVPGKKKLADFAVAIGKASLEHFSGYYGIPYPGDKLDLIAIPDFASGAMENLGA
                     ITFRETALLVDPEKATRAELERVADVVSHENAHMWFGDLVTMKWWNGLWLNEAFATFM
                     EMLAVDAWKPGWRRWDSFAVSRAAAMQVDGLRSTRPIEFPVDRPEEAAGMFDVLTYEK
                     GASVLRMLEQYLGAVKFRDGIRLYLKNHAYGNAETTDLWDALEESTQQPVRAMMDGWI
                     FQPGYPLVSVEKSGRTIRISQQIFRYLPDGSGNGRSWHVPIFLRAGTSAGVVSRILFL
                     TEKEQQVEFDAPADWVVVNAGAHGFYRVRYSNELLAALRQGMTTQLTAVERFGLVNDC
                     WAATLAGIVPLTDYLELIDQMSGETDVNVWTTMIASFHQMQRIFDDVQCKKLAGRLLA
                     VLSLALERLGWSVRPGEDELSSQLRADLIRALGTVADDPVTQDRARELFAAYEKNPNA
                     VDRNLTPALVAILAHIGGSEEYEKFFARFKTAQTPQEETRYLFALANFTDAELIDRTL
                     DLAINGMVRTQNSPYLMREALLNKHGRERAWSFMKAHWDEMNRQYPDNAIPRMCEGVV
                     GLVSAALERDVANFFHVHPVKQGSKQIEQHLERLRVAVACQERWRNLLRG"
     gene            3388..4077
                     /locus_tag="VE170_15490"
     CDS             3388..4077
                     /locus_tag="VE170_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ16901.1"
                     /translation="MSNAPRLTAGARREAIVAAVQDIFAEKGFDGTTIKELAHAAGVS
                     EALLYKHFPSKDSLYHAMLDACAKGPTFAEASRILEFEASTSTLVVMVHFMISHYVLG
                     RPGDTHRAALNSLLMRSLLDDGELVRLMHKKLAAAWLKKFEACLQASAKSGELHDVPF
                     RKDICVWFVQHVAFSLMLHLRPKVPAINYKVSREELVTQATWFALLGIGLKEDAARRY
                     YSPKALQLLAG"
     gene            4152..4583
                     /locus_tag="VE170_15495"
     CDS             4152..4583
                     /locus_tag="VE170_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16902.1"
                     /translation="MEFKAPSSNEGGKKTAGLDERIGSLFQPDTLIGEDYAANFRRKI
                     PLEAERTLLLAVLEDGIRSFQENLFATSGKRRTIFDEANEWIFSEDDTWFCSFVSVCN
                     LLSLEPQYIRRGLRQWEVKMHKSATQKKAQLAVGDDQRLVA"
BASE COUNT          997 a         1222 c         1447 g         1112 t
ORIGIN      
        1 aggggatcac ccaggaatat gccgcggtga tgctcagcgt ctttgccggg cttaacttga
       61 tcacgcactt cgtcctcggc tggattgccg accggatcaa caagcccaaa ctgctgtcgc
      121 tgtggatgct cttgccgatc gccggtatta tcatattgcg caccggcgac tcgcttgtgt
      181 cgctatggct gtttacgatc tttttctcga ccctggatgc ggcgtttccc gtcacctggg
      241 ccaccagcgg cgatttcttc ggccgcaagt acttcgctac cattcgcggc aatatgacgt
      301 tcgtctacat gtggggcagc gcccttggcc cggtgatcgc cggctatttg tacgaccaga
      361 cgcagagtta tggcgctatt ctatgggcta tcgtcgccac cttgtcggtt tcggtgatgc
      421 taacatcgct gttgatcaag ccctggaggg ataaggtccg gcatttgtcc acagcatcgc
      481 ggcggtcgtc cttgtcttga gcttttgcca agggtcggag ccttgagttc gaaaaattgc
      541 cgaactacga tgcggttact tgaatttatt cgatttcgaa atgaggcgta gaaatggctg
      601 aaccaagatc gcaccgtttg ccgacgtcgg ttatccctga gcgttatgag atcgagctaa
      661 ctccggattt gctccaatgg acctttaccg gtgaggtcaa gatcgccgta gatgtgcgcc
      721 aagaggtgcg tgaggtggtc ctcaacgcgg cggagttggt gatccagtcg gtttctctgc
      781 gaagcgccga cggcacgccg catcatggca acgtgcaagt ggacgacgcc aacgagcagg
      841 cggtattgag ctttcctgac cttgtagcgc cgggaaacta ccagttgcag attcatttca
      901 ccggggtgct caacgacaag ctgcatggtt tttaccgcag cgtgtacaag ggcgttgacg
      961 gtcaggacaa accgctggcg tcgacgcagt tcgaatcgac ggacgcgcgc cgggcgtttc
     1021 cctgttggga tgagccggcg ttcaaggcgg tgtatcaggt gactttggtg attgacgaaa
     1081 aactcacggc gatttccaac gcccgagtca tcgaagagac ggtgttaccg ggaaccggta
     1141 aaaaggcggt gagattcgcc gactcaatca aaatgtcgac ctatctagtg gcgtttatcg
     1201 tcggtgattt cgaagccacc gaacccatga cggcggatgg tgtgccgctg cgggttctgg
     1261 cggtgccggg taaaaaaaaa ctggccgact tcgccgtcgc catcggcaag gcgtcattgg
     1321 aacattttag cgggtactac ggcatccctt atcccggcga caaattggat ttgatcgcca
     1381 tccccgactt cgcctcgggc gccatggaaa atttgggcgc cattaccttt cgtgaaaccg
     1441 cgctgttggt cgatcccgaa aaagccaccc gtgccgagct cgagcgcgtc gccgatgtcg
     1501 tgtcccatga aaatgcccat atgtggtttg gcgatttggt gacgatgaag tggtggaatg
     1561 ggctgtggtt gaacgaagct ttcgccacct tcatggaaat gctcgcggtc gacgcctgga
     1621 aaccggggtg gcgccgctgg gatagttttg ccgtgtcgcg cgccgcggcg atgcaagtgg
     1681 atggtctcag gagcacccgg ccgatcgagt ttcccgtcga taggccggag gaggcggccg
     1741 gcatgttcga cgtgctcacc tacgaaaagg gcgcttcggt gttgcgcatg ttggaacagt
     1801 accttggcgc tgtaaagttt cgcgatggaa tccggctgta tttaaaaaac cacgcttatg
     1861 gcaacgccga gaccaccgat ttgtgggacg ccttggaaga gtcgacgcag cagccggtgc
     1921 gcgccatgat ggacggctgg atcttccaac cgggctatcc gctggtcagc gtcgaaaaat
     1981 ccggtcggac catccgcatt tcccaacaga tctttcgtta tttaccggac ggcagcggca
     2041 acgggcgcag ctggcatgtg ccgatttttc tgcgtgctgg aacctcagcg ggagtcgtta
     2101 gccggatttt attcctgacg gaaaaagagc agcaagtcga attcgacgcg ccggccgatt
     2161 gggtcgtggt aaacgccggc gcccatggct tttatcgtgt ccggtacagc aatgaattgt
     2221 tggccgcgct gcgccaggga atgactaccc agttgactgc ggtggagcgt ttcggtctgg
     2281 tcaacgactg ttgggcggcg actctggcgg ggatagttcc actgaccgat tatcttgaat
     2341 tgatcgacca gatgagcggt gagactgacg tcaacgtgtg gacgacgatg atcgcatcgt
     2401 tccaccaaat gcagcggatt ttcgatgacg ttcaatgtaa aaaattggcg ggacggctct
     2461 tggctgtgtt gtcactggcg ctcgagcggc tcggttggtc ggtacggccg ggcgaggacg
     2521 aattgtcgag ccagttgcgc gccgatctga tccgtgcgct tggcacggtc gccgacgacc
     2581 cggtgaccca ggatcgcgcc agggagttgt ttgccgccta cgaaaaaaac cccaatgcgg
     2641 tggatcgcaa tttaaccccg gctttggtgg cgattctggc gcatatcggc ggtagtgagg
     2701 agtacgagaa gttttttgcg cgatttaaaa ctgcccaaac gccgcaggag gagacgcgtt
     2761 atcttttcgc gttggccaat tttaccgacg ccgagttaat cgatcggact ttggatttgg
     2821 cgatcaacgg tatggtgcgg acgcagaact cgccctacct gatgcgcgaa gcgctgctca
     2881 ataagcatgg ccgggagcgc gcttggtcgt tcatgaaagc gcactgggat gaaatgaatc
     2941 gccagtatcc ggacaacgcc atcccgcgca tgtgcgaagg cgtcgtcggc ttggtcagtg
     3001 ccgcgcttga aagagacgtg gctaattttt ttcacgtcca cccggtcaag caggggagca
     3061 agcagatcga acaacatctt gaacgtttgc gcgtagcggt ggcttgtcag gaacgatggc
     3121 gcaacctgtt gcgcggctag tatcggcgct ttgagcggcg cttagcgttg gcgttcactc
     3181 atgtcgcgaa gtaaagcgaa accctatttt cgcctacaat caaccgctaa tttacgtcgt
     3241 cggaaggtca tcatctctct ttagacagcc ttgccgtttt gttatattcc ttagtaaatt
     3301 tagatagtta ggccagctat tacgggctgg gtaaaagttg aataatttgc cttgacagga
     3361 caagtgagta aatgctaact aaccggtatg agcaacgctc ctagacttac cgcaggggcg
     3421 cgccgcgaag cgatcgtcgc agcggtgcaa gacattttcg cggaaaaagg cttcgacggc
     3481 acgaccatca aagagttggc tcacgcggcg ggagtttccg aagcgttgct ttataagcat
     3541 tttcccagca aggattcgct ctatcacgcg atgctcgatg cctgtgcgaa ggggccgaca
     3601 tttgccgagg cgagccgcat cttggaattt gaagcatcga cttcgacctt ggtggtgatg
     3661 gttcatttca tgatctcgca ctatgtgctg ggacggccgg gcgataccca tcgagccgcg
     3721 ctcaattcgc tgctaatgcg tagtttgctc gacgacggcg agttggtgcg tttgatgcac
     3781 aagaagctcg ccgcggcgtg gttgaagaag ttcgaggctt gcttgcaggc atcggcgaaa
     3841 tcgggcgaat tgcacgacgt gccatttcgc aaagatattt gcgtttggtt cgttcagcat
     3901 gtcgcttttt ctttgatgct gcacctgcgg ccaaaagtgc cggcaatcaa ctataaggtt
     3961 tcgcgcgaag agctggtgac ccaagcgact tggttcgcac tcttggggat cggccttaaa
     4021 gaggatgcgg cacgccgtta ttacagcccg aaagccctgc aattactcgc cgggtgatgg
     4081 ggttattgat gatggggtta ttacggttgg tttaaaaatc tgatttaatt ctttttaaaa
     4141 ggggaggggt tatggagttc aaggcaccta gcagtaatga gggcggcaaa aaaaccgccg
     4201 gtttagacga gcgcattggc tcgctatttc agcccgatac gttgatcggc gaagactatg
     4261 cggccaattt tcgccgcaag atcccgctgg aggcagagcg caccttgctg ctggcggtct
     4321 tggaagacgg catccgttcg tttcaagaaa atctcttcgc caccagcggc aagcgccgaa
     4381 cgattttcga cgaagccaac gagtggattt ttagcgagga cgacacctgg ttttgctcct
     4441 tcgtgtcggt gtgcaacttg ctcagtctgg agccccagta catccgccgg gggctgcgcc
     4501 agtgggaagt caagatgcac aagagcgcga cacaaaagaa agcgcaactc gcggttggag
     4561 acgatcaacg cttagtggcg tagttagaag cggcgtgaca cgaatcttgc ggcgccgagg
     4621 ggtgcaatgc tcttgggcat ttgcacttct cagcgccggt ttatttttgg cctgcgggaa
     4681 gaaaagcgag accgctttgc agcggccccc cgcgccggtg gcggtggtgc aggcggtcat
     4741 tcaagacgta ccggtataca tcgatgctgt cggcaaga
//
LOCUS       DASQKS010000604         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_300435, whole genome shotgun sequence.
ACCESSION   DASQKS010000604 DASQKS010000000
VERSION     DASQKS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300435"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..819)
                     /locus_tag="VE170_15500"
     CDS             complement(34..819)
                     /locus_tag="VE170_15500"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16903.1"
                     /translation="MLSDLQLGHERFGAAIRCEAVSRWFAGSGLVLDQISFQVDPGEF
                     VALLGPSGSGKSSLLRLTAGLDQPNSGALVIESAGGALSRGFVFQEATLMPWRTVLRN
                     TSLPLELAGWSRADAEGRATEELGRVRLTEVAARYPHELSGGMKMRTSVARALTTRPN
                     LLLLDEPFAALDEPARHALQIQLREIWRALKMTVLFVTHSVTEAVFLADRIIVLSQRP
                     ARLLLDQRVALPDNRDTELRTSLEYITEVRRVATAVPAGGQPS"
     gene            complement(832..1866)
                     /locus_tag="VE170_15505"
     CDS             complement(832..1866)
                     /locus_tag="VE170_15505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16904.1"
                     /translation="MDASYAAIDADGHVMETDDELRRYLPPPFEGRRALTALFPSLDG
                     WPRSTRKAPDPTPCLARWRTFLNASGVAGSVLYPTMGLAMAHIKDVQWATVMARCYND
                     YLYGEYLAQEPERLWGVALLPIQDVAASADELERCVKDLKMVGGVIPAVGLAKGLGHP
                     MYDPLYRTAVRLNVPLAVHGGSSAGLGLDILDSFVKILVLEHAVAQQIHFTSFLLDGG
                     PVRFPDLKIAFLEAGVGWVPYLMERLDEKYEKLPHQAPLLTKAPSEYVKDGAIYFSCE
                     LEERILSYVIGLGLEKKIMYPSDFPHERPTLEQFLADIPHFQAREDLSDKVKKLILRD
                     NCVDFYSLRI"
     gene            complement(1879..2817)
                     /locus_tag="VE170_15510"
     CDS             complement(1879..2817)
                     /locus_tag="VE170_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16905.1"
                     /translation="MQTTNGSAEPLMVGYSAVSSVFLPFWIGKEAGFYKKEGLDAQLV
                     YIASSTTMAQAMFARQVAISSVNSGSVVTSTLQGGDLVLMGAVMNMAGFYIMTRPEIA
                     NVQELRGKKIGVTRLGSSSDFAIREYLQRNKLNPGRDVNIVQIGGMPELAAALNNGSI
                     SAAPLSAPSSHVAEQRGNRIIANLANEGIYFVIAGLTTTRRYLREQRGEAKAFLRAFG
                     RATHFMFQQKEEAKKLLTKYAKIDDAGMLEGSMKYVYDFTEKIPLVRREGVQVVLDQE
                     TAKMPQAKDFTAERFYDNSLVQELISEGFYKALWGK"
BASE COUNT          678 a          911 c          860 g          602 t
ORIGIN      
        1 gcggcaacca tgacgcaaag cgcgctctga ttgtcatgaa ggttgtccgc ccgccggcac
       61 cgcggtagcg acacgccgga cttcggtgat gtattccagc gatgtgcgca gctcggtgtc
      121 gcggttgtcg gggagcgcga ccctttgatc gaggagcagt cgcgccggac gctgggacag
      181 aacgatgatc cggtcggcga gaaacaccgc ctcggtaacc gaatgagtga caaacaatac
      241 ggtcattttg agcgcgcgcc aaatctcgcg cagctgtatt tgcagcgcat gacgggccgg
      301 ctcatcgagc gcggcaaatg gctcatcgag cagcagcaag ttcggccggg tcgtcaaggc
      361 gcgcgccacc gaagtacgca tcttcatgcc gccggagagt tcgtgtggat aacgcgcggc
      421 aacctcggtc aagcgtaccc gccccagctc ttcggtcgcc cggccctcgg catccgccct
      481 cgaccaaccc gccaattcca ggggcaagga agtgttgcgc agcacggtgc gccatggcat
      541 cagcgtggct tcttgaaaaa caaaaccgcg cgacaacgcg ccaccggctg attcaattac
      601 gagggcgccg ctattcggtt gatcgagtcc agcggtgagg cgaagcagag aagatttccc
      661 cgagccggaa ggcccgagca aagcgacgaa ttcaccggga tcgacttgga aggaaatttg
      721 gtcgagtacc aaaccgctgc cggcgaacca gcgcgaaacc gcctcgcaac ggattgccgc
      781 gccgaaccgc tcgtgaccta gttgcaaatc ggagagcatc gacgctgtcg gtcaaatccg
      841 caacgaataa aaatcgacac agttgtcgcg caggataagt tttttgacct tgtccgaaag
      901 gtcttcgcgc gcctggaagt ggggaatgtc ggcgagaaac tgttcgaggg tcggccgctc
      961 gtgcgggaag tccgagggat acatgatctt tttctcgaga cccaaaccga taacgtagga
     1021 gagaatgcgt tcttccaact cgcaagaaaa gtagatcgcc ccgtccttga cgtactcgct
     1081 cggcgctttg gtcaggagcg gcgcttgatg cggcagcttc tcgtattttt cgtcgagccg
     1141 ttccatcaaa taaggcaccc agccaacgcc ggcttcgagg aaagcgatct tgagatcggg
     1201 aaatcgcacc ggaccgccgt cgagaagaaa actggtaaaa tgaatctgtt gcgccacggc
     1261 gtgttcaagg acgaggatct tcacgaacga atcgagaata tccaagccga gacccgcgct
     1321 cgatccgcca tgcacagcca gcggcacatt caatcgcacg gcggtgcgat agagcggatc
     1381 gtacatcgga tggccgaggc ctttcgccaa accgaccgcg ggaatcaccc cgccgaccat
     1441 tttcaaatcc ttgacgcatc gttcaagttc gtcggctgaa gccgcgacat cttgaatcgg
     1501 cagcaacgcc acgccccaga gccgctcggg ctcctgggcg agatattcgc cgtagagata
     1561 gtcgttgtaa cagcgcgcca tcaccgtcgc ccattggacg tctttgatat gcgccatggc
     1621 caaccccatg gtcggataga gcaccgaacc cgccactccg gaggcattta gaaacgtccg
     1681 ccatcttgcc aaacaaggcg tcgggtccgg cgccttgcgc gtgctgcgcg gccagccgtc
     1741 gagggatggg aacagcgcgg ttagcgcgcg ccggccctcg aagggcggcg gcaaatagcg
     1801 gcgcaattcg tcatcggtct ccatcacgtg cccgtcggca tcgatcgctg cataactcgc
     1861 gtccatccaa actccctttc acttacccca aagcgctttg tagaatcctt cgctgatcag
     1921 ctcctgcacc aagctattgt cgtagaatcg ctcggcggtg aaatcctttg cctggggcat
     1981 cttcgccgtc tcttgatcga gcaccacttg cacgccctca cgcctcacta ggggaatctt
     2041 ctcggtaaag tcatagacgt atttcatgct gccttcgagc atgccggcgt catcgatctt
     2101 ggcatatttt gtcaggagct ttttcgcctc ttccttttgt tgaaacatga agtgggtcgc
     2161 gcggccaaac gcgcgcagaa aagctttggc ttcgccgcgt tgctcgcgca gatagcggcg
     2221 cgtcgtcgtc aaaccggcga tgacaaagta aattccttcg ttagcgaggt tggcgatgat
     2281 tcggttaccc cgctgttcgg cgacatggga actcggcgcc gacaatggcg ccgcgctgat
     2341 cgagccatta ttgagcgccg cggccaattc cggcatgccg ccgatctgga cgatgtttac
     2401 gtcccggccg ggattcaatt tatttcgttg cagatactcg cgaatggcga agtccgaaga
     2461 tgagcccaag cgcgtcacgc cgattttctt gccgcgcaac tcctgaacgt tggcgatctc
     2521 cggccgggtc atgatgtaaa atccggccat attcatcacc gcgcccatga gcaccagatc
     2581 gccgccctga agcgtgctgg tgaccacgct gccgctattg accgatgata tcgccacctg
     2641 gcgggcaaac atcgcctgcg ccatggtcgt cgagctggcg atgtaaacga gctgggcgtc
     2701 gaggccttcc ttcttgtaaa aacccgcttc cttgccgatc cagaatggca gaaacaccga
     2761 actcaccgcc gagtaaccga ccatcagcgg ctcggcagac ccatttgtcg tctgcatgtg
     2821 ccagcacaac atcaaacaaa atatcgatcg taatgcaggc ttcatgatca tcggcacttt
     2881 ccaatgagcg caagctaaac ggaattcatt gatttggcaa ttggcttgct gtgcgaaatc
     2941 ccaactttca attgacacca acccgatagg gcggtatgcc aagcaacatt cgtacacgca
     3001 tagaaccggc ggcggagaaa taatcggggg ataaatttca tgagcgcaaa g
//
LOCUS       DASQKS010000605         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3287036, whole genome shotgun sequence.
ACCESSION   DASQKS010000605 DASQKS010000000
VERSION     DASQKS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3287036"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VE170_15515"
     CDS             complement(<1..704)
                     /locus_tag="VE170_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYJ16906.1"
                     /translation="MNEAERAYNAAEFTAGASAANPGATMPAQVGVAAPEFEAETLDG
                     KIVRLSDFRGRSHVVMMTGAVTSPMCAFEVPELNQLQADFAAEGVEFFLLYSRESHPA
                     ENYGAHTSLEQKRSHARELQRLEDVRFAVIVDHLDGRIHCAYGVWPNALFVIHKDGRL
                     VFRSNMANHRELRQFLEDMVAAERAVAAGRVVHLQYSERVVAHEADQTTHRRVYERAG
                     AQAFEDYWAKRPQHRNR"
     gene            complement(720..1736)
                     /locus_tag="VE170_15520"
     CDS             complement(720..1736)
                     /locus_tag="VE170_15520"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ16907.1"
                     /translation="MTTAQTIDLKNLDEELAKDNLRGLWAREESIRREPAPFGKPILW
                     KWAKIRAGLEAAGQLITTNYKGARRAISLVHPNMSDSTSHTLNLAVQLVKAGEAVYSH
                     RHTNAAMRFVIDGGARVYTITNGEKCVMERGDLVIQPSWGWHNHVNETERDAIWIDGL
                     DSGLMRMLRTMFQEPHPAEDARLYNSPADTTLRNPGLLAPPGQPLQSLVYKWTESRRA
                     LAEMLADSANPFDGRCLEYRNPATGGSTFPTFSCWIQMLDGNQETAEHRHTSNQLYYA
                     VEGSGVCEVESEKLVWEAGDFFVVPNWSWHHHKNTSAAPAVLFSTTDRPLHEAIGVYR
                     EQSR"
     gene            complement(1864..3054)
                     /locus_tag="VE170_15525"
     CDS             complement(1864..3054)
                     /locus_tag="VE170_15525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ16908.1"
                     /translation="MKYQCISADSHLEIRPDRYAKRVPARFRDRAPKVITLEDGSLAV
                     LQEGQPLERLISNISCGLPYEERRPFDPLPGENYENSPGTGSPEQRLREQDQDGVDAE
                     VLFPGNVGPGFWRGIRNDEVYKSVVRAYNDWLAEEYCSFSPDRLIGVGVIPITNIGDA
                     VAELQHCRRIGLKAVAIDNFPAGNRQPTAADDPFWAAVVDLQMALTIHVEFGFPRRGR
                     GQPAVAAPAFKYAKQTDPEHHVPDVIERFNKYGFRGSKQVVQMIWGGVFDRFPKLNIY
                     VAEVQIGWVPNWLDQMDNEYGRQQYWAERVLGLPRLKRMPSEYGREFVHWGFNRNPIG
                     VRIARQELGVDKVMWANDFPHLESDWPNSRRVLAENFAGVGEDEIWKMTVGNAAKYFH
                     LADG"
BASE COUNT          641 a         1006 c          952 g          652 t
ORIGIN      
        1 cggttacgat gctggggccg cttggcccaa tagtcctcga acgcttgcgc cccggcgcgc
       61 tcgtaaacgc gccggtgggt cgtctggtcg gcttcgtgcg ccaccacccg ctcggagtat
      121 tgcagatgca ccacccgccc ggcagcgacg gctcgttccg cggcaaccat atcttcaaga
      181 aactggcgca gctcccggtg attcgccatg ttgctgcgaa aaaccaaacg gccgtccttg
      241 tgaatcacga ataaagcgtt gggccaaacc ccatacgcgc agtgaatccg gccatcgagg
      301 tgatcgacga tgacggcaaa ccgaacatct tcgagccgct gcaactcgcg ggcgtgagag
      361 cgtttctgct ccaagctagt gtgcgcgcca taattctccg ccggatgaga ctcccgcgag
      421 taaagcagaa agaattccac gccctcggcg gcgaaatcag cctgcagctg attcaactcc
      481 ggcacttcga aggcgcacat cgggctggtc accgccccgg tcatcatcac cacatggctg
      541 cgaccgcgaa agtcgctcag ccggacgatc ttcccatcca aagtctcggc ttcgaactcc
      601 ggcgcggcaa cgcccacctg cgccggcatg gtcgcgcccg gattggcggc gctcgcccca
      661 gcggtgaact ctgctgcgtt ataggcgcgt tctgcttcgt tcatttgcct gcggcgaatt
      721 taccggctct gctcacggta cacgccgatc gcttcatgga gcggccggtc ggtggtggaa
      781 aacaacaccg cgggcgcggc ggaggtattt ttatgatggt gccaggacca gttgggcacg
      841 acgaaaaagt cgcccgcttc ccaaaccagc ttctcgctct ccacctcgca gacgccgctg
      901 ccttccaccg cgtaatacag ttggttcgag gtatggcgat gctcggcggt ctcctggttg
      961 ccgtcgagca tttggatcca acaggaaaaa gtcgggaagg tcgacccgcc ggtggccgga
     1021 ttgcgatact ccagacagcg gccgtcaaat gggttggcgc tgtcggcgag catttccgcc
     1081 agcgcgcgcc ggctctcggt ccatttgtac actaacgatt gcagcggctg tccgggcggc
     1141 gcgagcaagc cgggattgcg cagcgtggta tcggccgggc tgttgtacag ccgcgcatct
     1201 tcggccggat ggggttcctg aaacatcgtc cgcaacatgc gcatcaagcc tgagtccaag
     1261 ccgtcgatcc aaatcgcgtc gcgctcggtt tcattgacgt gattgtgcca accccagctc
     1321 ggctgaatta ccaaatcgcc gcgctccatg acgcacttct cgccgttggt gatggtatag
     1381 acgcgggcgc cgccgtcgat gacgaagcgc atggcggcat tggtatggcg atgggagtag
     1441 accgcttcgc cggctttgac cagctgcacc gccaggttga gcgtatggct ggtgctgtcg
     1501 ctcatgttcg ggtggaccag actgatggcc cggcgcgcgc ccttgtagtt ggtcgttatc
     1561 agttgcccgg cggcttcgag accggcgcga atcttcgccc acttccaaag aatcggcttg
     1621 ccgaagggtg ccggctcgcg ccgaatcgat tcctcacgcg cccacaagcc gcgcaaatta
     1681 tccttggcca gttcttcgtc gagatttttc aggtcgatgg tttgcgcggt ggtcacaatc
     1741 tgctcctcgg tttgccgtaa tccatgaatc gctttgacgg ctgcccagcg atcgacccct
     1801 atggatgaaa cgcgcggcgg cgccggtggt taggtcattt cgctcgcagg aagaaatctc
     1861 gccttagcca tcggccaagt gaaaatactt cgccgcgttg cccacggtca ttttccagat
     1921 ctcgtcttcg ccgacgccgg cgaagttttc cgcgagcaca cggcgcgagt tgggccagtc
     1981 cgattcgaga tgaggaaagt cgttggccca catcactttg tccacgccca actcctggcg
     2041 cgcgatgcgc acgccgatgg gattgcgatt gaaaccccag tggacgaact cgcgtccata
     2101 ttcactgggc atgcgcttca gccgcggcaa accgagcacg cgctcggccc aatattgctg
     2161 ccggccgtac tcattatcca tctgatcgag ccaattgggc acccagccga tctgcacctc
     2221 ggcgacgtaa atattcaatt tcggaaaacg atcgaacacc ccgccccaga tcatctggac
     2281 aacttgcttg ctgccgcgaa aaccatattt attgaaacgt tcgatgacgt ccggcacatg
     2341 atgttcggga tcggtctgct tggcgtattt aaacgccggc gccgccaccg ccggctggcc
     2401 gcggccacgg cgcggaaagc cgaactcgac gtgaatcgtc aaggccattt gtaaatcgac
     2461 caccgcggcc cagaagggat cgtccgccgc cgtcggctgg cggttgccgg cgggaaagtt
     2521 gtcgatcgcc accgctttga ggccgatgcg ccgacagtgc tgcagctcgg cgacggcatc
     2581 gccgatgttg gtaatcggaa tcacgccgac gccgatcaag cggtcagggg aaaacgagca
     2641 atactcctcg gcgagccaat cgttgtaagc gcgcactacc gacttgtaga cctcatcgtt
     2701 gcgaatgccg cgccagaatc cgggaccgac gttgccggga aacaacactt cggcgtcgac
     2761 accatcctgg tcctgttcgc gcaagcgttg ctcgggcgag ccggtgccgg gagagttttc
     2821 ataattttca ccaggaaggg gatcgaaagg acgccgttct tcatagggca ggccgcagga
     2881 aatgttggag atcaaacgct ccaatggctg gccttcctgc agcaccgcca gcgagccgtc
     2941 ttccaaggtg atcaccttgg gcgcgcggtc gcggaacctt gccggcacac gcttggcgta
     3001 acggtcggga cggatctcca aatgggagtc ggcggaaatg cattgatact tcataaatcc
     3061 tcggatcagt cagtcagcgc ccggattaca ccggcaaacc cgggttgtca atgtacgccg
     3121 gcaagacggc gaaaaatcat tgggctcacg agccgaggca aaatcaaacc gaactatctt
     3181 tcggcaaatc cccgccggct atgcggcttg aattaccctg tgaatggaga ttatgtaatt
     3241 tttacttctt c
//
LOCUS       DASQKS010000606         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3711262, whole genome shotgun sequence.
ACCESSION   DASQKS010000606 DASQKS010000000
VERSION     DASQKS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711262"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1178
                     /locus_tag="VE170_15530"
     CDS             213..1178
                     /locus_tag="VE170_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HYJ16909.1"
                     /translation="MANLSLTMALSHYDRHVPLFDGSVTVENVDLRVLEVGQSDSLKH
                     GKDRHERMLQKGEFDICELSLSNYLVARSRNMPFTAIPVFPRRLFSLSQMWVNTGAGI
                     TSPRDLIGKKVGLSTFQTTLSVLARGDLQADYGVPWREIEWIISKDEAVPLKPLAGVK
                     MQLVKPGQKMGAMLEQGEIDAIMMPHPPKEALRSDGKIRRLFADPKAEETKYFHKNGY
                     FPIMHVVAFKDQLLKQNPWLAQSVTAAFDKAKAACMEYYDDPNWSRFVWGRQLFEEER
                     KVFGDDPWPHGIAKNRANLERFIGYSFDQGLIEKQLTVEELFFEK"
     gene            1202..1393
                     /locus_tag="VE170_15535"
     CDS             1202..1393
                     /locus_tag="VE170_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HYJ16910.1"
                     /translation="MPLTIKCPTCRDETTWENNPHRPFCSKRCQTTDLGAWLEERYKI
                     PGDNFELLQQNDDDDDDDE"
     gene            1815..2591
                     /gene="lpxA"
                     /locus_tag="VE170_15540"
     CDS             1815..2591
                     /gene="lpxA"
                     /locus_tag="VE170_15540"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HYJ16911.1"
                     /translation="MSIHPTAVIDSKAEIDPSVEIGPYVVIDSAVKIKRGTRVMAHAY
                     LTGLTEIGADNEIHPGAVIGATPQDTAYKGQETYLRIGDRNIIREYAQIHRGTAAGSA
                     TVLGNDNYLMATSHVGHNCVLADNVILANGALLAGHVQVGKSVFISGNCVVHQFVKIG
                     DYALMRGLSGTSRDVPPFAIIDWQHTVRGVNVVGLKRAGFDEQRIRAIRQAFRTLFRS
                     GRNLAVAVKEVRESGRVNTDVLALLEFIDSSKRGVCFGAD"
     gene            complement(2588..>3023)
                     /locus_tag="VE170_15545"
     CDS             complement(2588..>3023)
                     /locus_tag="VE170_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16912.1"
                     /translation="DSNTTLRYGLCMKSQMLKGIALASLVTLVSTPLYAQTAGTNVQP
                     AEPVAEELGWGFGAALASVFYIPAKVTYAGLGLLTGGLGYVLTGGRADVANNIIYPSV
                     RGNYVVTPNHLKGTEPIYFVGAGPDAPEPETIASGPAPAQRP"
BASE COUNT          709 a          801 c          851 g          662 t
ORIGIN      
        1 tgaaattttc gacggccgat aaagtattta agactaactc caagtcggac atcacgagct
       61 cgcctgctgt cgatctaaat tcggcccgta ctcacgtatt cgtcattgtt tgccactgtt
      121 tttcacgtca gttcacttta cgaccgattg cgggctggaa agcgttggaa aagtgcagta
      181 agcagtaggc atcacagcgg cgaggtaacg agatggccaa tttgtcttta accatggcgc
      241 tttcccacta cgaccggcat gtgccgttgt tcgacggctc ggtgacggtg gagaacgtcg
      301 acttgcgagt gctcgaagtc ggccagtcgg attcgctcaa gcatggcaag gaccgccatg
      361 aacgcatgct gcaaaagggc gaattcgaca tttgcgagtt gtcgctgtcg aactatctag
      421 tcgccaggag ccgcaatatg ccgtttacgg cgatcccggt atttccgcgg cggctgttca
      481 gtttgtcgca aatgtgggtg aataccggcg cgggaatcac ttcgccgcgg gatttgatcg
      541 gcaagaaagt cgggctcagc acctttcaaa ccaccttgtc tgtgctagcg cggggtgatt
      601 tgcaagccga ctatggcgtt ccctggcgcg agatcgagtg gatcatttcc aaagacgaag
      661 ccgtgccgct caaaccgctc gcaggcgtca aaatgcaatt ggtaaaaccc ggccagaaga
      721 tgggcgccat gctggagcaa ggtgagatcg atgcgatcat gatgccccat ccgcccaaag
      781 aagccttgcg cagcgatggt aagattcgcc gtctgttcgc ggacccgaaa gccgaagaga
      841 ccaagtattt tcacaaaaac ggttatttcc cgatcatgca tgtggtggcg ttcaaagatc
      901 agctcttaaa gcagaatcct tggctggcgc agtcggtgac tgcggcgttc gacaaggcca
      961 aggctgcctg catggaatat tacgacgatc ccaactggtc gcggtttgtc tggggacgcc
     1021 aattattcga ggaagaaaga aaagtttttg gcgatgatcc ctggccccat gggatcgcga
     1081 aaaaccgcgc gaacctggag cgctttatcg gctactcgtt cgatcaggga ttgatcgaaa
     1141 aacaactgac cgtcgaagaa ttattttttg aaaagtaaat ggcatagacg gcgagaaaac
     1201 catgccgctg acgatcaagt gtccaacttg tcgtgacgag accacttggg agaacaaccc
     1261 gcatcgcccg ttttgttcga aacgctgcca gaccaccgat ctgggcgcct ggcttgagga
     1321 acgctataag attcctggcg ataatttcga actactccag caaaacgacg atgatgacga
     1381 cgacgatgaa tgacgctcga taggatgagg ctcggggaaa ggcgctaatt acaaaaattc
     1441 gaagcacgaa gtgttcatga tcttacgctc gtctacataa tatgaaaatg gatgggattc
     1501 ctccataggc ggtctgcttg acgcgtcatt cccgcgcacg cgggaatcca ggtttggtct
     1561 gcgccgaatt agcctggata cccgctttcg cgggtatgac ggaacccata tggcctcttc
     1621 attgtggaga ccctcccctt acatgtattt tccaaggagc acgaaacaaa ttcaaatgac
     1681 caggagatac aaaattccaa acacccctgc ttcggatgcc tcgttctgga ttttcccaaa
     1741 tttgatttat ttgactgtaa gtttgtttcg aatttcgtgc ttcggatttt ctatcgctga
     1801 gagtttgcaa acccatgagc attcacccca cggcagtgat cgattcaaaa gccgagatcg
     1861 atccgtcggt agagatcggc ccctatgtcg tgatcgacag cgcggtcaag atcaagcgcg
     1921 gcacaagggt gatggcccat gcctacctca cgggtttgac cgagatcggc gcagacaatg
     1981 agattcatcc cggcgcggta atcggcgcaa cgccgcagga tacggcctac aaggggcaag
     2041 aaacttatct gcggatcggc gaccgcaaca taatccgcga gtatgcgcag attcatcgcg
     2101 gcacggcggc cggttcagcg acggtgctgg gcaatgataa ttacttgatg gcgacttccc
     2161 acgtcggtca taactgcgtg cttgccgaca acgtgattct cgccaacggc gctttactcg
     2221 ccggtcatgt gcaagtcggc aagagtgtgt ttatttccgg caactgcgtg gtgcaccagt
     2281 tcgtcaagat cggcgattat gcattgatgc gcggtctgag cggcaccagc cgtgacgtgc
     2341 cgcccttcgc gatcatcgac tggcagcata cggtgcgcgg cgtaaatgtg gtcgggctca
     2401 agcgcgcggg attcgacgaa caacggatcc gggcgatccg ccaggccttt cgcacactat
     2461 ttcgcagcgg aagaaacctc gccgtagcgg tcaaagaggt aagagagagc gggagggtca
     2521 acaccgatgt gctggccctc ttggagttta tcgactcttc gaagcgggga gtttgcttcg
     2581 gcgcggatta ggggcgctgt gctggcgccg gaccgctggc aatggtctcg ggctccggcg
     2641 catcgggacc ggcgccgacg aaatagatcg gctccgtgcc cttcaagtgg ttcggggtca
     2701 ccacgtagtt gccgcgcacc gacggataga tgatgttgtt ggcaacatcc gccctgccgc
     2761 cggtaagcac ataaccaagg ccgccagtga gcagtcccaa tccggcatag gttaccttcg
     2821 ccgggatgta aaacacgctg gcgagagccg cgccaaaacc ccagcctaac tcctccgcca
     2881 ccggttcagc aggttgaaca ttcgtccccg ccgtctgggc atacaatgga gtcgacacta
     2941 gggtcaccaa tgaagccagg gcaatgccct tgagcatttg tgatttcatg caaagaccat
     3001 atcgcagggt agtgttactg tca
//
LOCUS       DASQKS010000607         5179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1999583, whole genome shotgun sequence.
ACCESSION   DASQKS010000607 DASQKS010000000
VERSION     DASQKS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999583"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VE170_15550"
     CDS             <1..305
                     /locus_tag="VE170_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16913.1"
                     /translation="EVYHMVRATLRGLQFVSDEANREQARDVMMKQWRVSDQKLAAEM
                     LGYLKRGLAKDAVISPQAVQYFVDLTRETSNVAQPIPGAQVVDFSFLEKARKDLVR"
     gene            326..958
                     /locus_tag="VE170_15555"
     CDS             326..958
                     /locus_tag="VE170_15555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HYJ16914.1"
                     /translation="MLEFAGRQIPTELKEIVDPKRSVLLVWDMQNDQAGGAFNKEALI
                     RTAPPLIAAAANAGVKTVYTRQTPFLWSEEAVTWIRRAMINEKVDHPSKLKPRRLKGS
                     TNWEIMKPFLPAPEDIVIDKRRPSMFIGNEFETIMHNRGASTVVMIGCTTDGGVEMTV
                     RDGHNRGFSMVIVGDCVGTYTEEGHRAALKRMERFADVVESKDLIEIWKS"
     gene            959..1369
                     /locus_tag="VE170_15560"
     CDS             959..1369
                     /locus_tag="VE170_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16915.1"
                     /translation="MASVHEIKDIIETFRAGDEESAFFTLIELSGDVLPALIETFRDE
                     KSSGIRAFLVKAAWERREEAVLPFLSEVLHEAEEEAWQQALDGLVAFATPQSLEILHA
                     ARSRSFTEKSAARRFLLWLDEATEQIKSELRAKK"
     gene            1461..2429
                     /locus_tag="VE170_15565"
     CDS             1461..2429
                     /locus_tag="VE170_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16916.1"
                     /translation="MSGTNGKKALHVATFSGWYRFEQNGKQFTQTKRALSYWTLTCMT
                     VDAKDPKTIYAGTEHSGLFFTKDAGAHWNRAEPQVPKMFLYSALAIDGGVMVGTIPSA
                     VYRSKNGGWEELEGVRLHSAGANFPPSPELQSRTRYLAYEPENQNRLYAGIEVGGMVL
                     SDDGGRGWEPCNDGLTDMDIHEILASEQHSGMVFLACGEACFRSPDRAGHWENISPKH
                     HDYGISVAEDNRGVLYVGAARGRPNLWLREEGAMSAILRSSDKGKTWDTVIDNLTGGV
                     MHLCPTPDGNGMIAGTSDGTLLLVDDSGAREVASGLPFVTSVELAA"
     gene            2500..3492
                     /locus_tag="VE170_15570"
     CDS             2500..3492
                     /locus_tag="VE170_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ16917.1"
                     /translation="MRILIGLYLCLCLPSHLLAQDAKLIAEGRKDGKVVIYGSMETDI
                     FDGVQQGFEKKTGIKVDYWRAAGATVLERASSEKRANKIGYDLVLNNAGPMEILLSEG
                     ALAKYDSPMAKNFAAEFQHPQLGPSYRTSIVGIVYNKSIISPEKAPKSLEDLLKPEFR
                     GKLAFADPSRGAVAVMWPMSLYKLMGKDRADKYLRDLGATKPVIVEGVLPAAERVTTG
                     ETPIAITYLKYVIVFGQKGAPLDYVRQDKLLGHGHAIAMSSRAARPNAAKAFLDYFYS
                     DESLRVMAKSGEFVTRKGIHPPLADADKIQVVEMDEPDANMMAEKRKEYRKIFQ"
     gene            3489..4133
                     /gene="maiA"
                     /locus_tag="VE170_15575"
     CDS             3489..4133
                     /gene="maiA"
                     /locus_tag="VE170_15575"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HYJ16918.1"
                     /translation="MKLYTFFRSSASYRVRIALNLKGLSCEQAPIHLRRGGGEQLMPS
                     YMALNPQALVPALEDNSRILTQSLAIIEYLDETHPTPPLLPKEPADRALVRSMAMVIA
                     CEVHPIQNLRVLQYVKARYNQTDEQVNQWAQHWIDLGLAALQEMIVAQPKRGKFCFGA
                     VPTLADICLVPQLGNARRYGCDLAKYPTILEIETNCNAIPAFASAAPENQPDAE"
     gene            4240..5013
                     /locus_tag="VE170_15580"
     CDS             4240..5013
                     /locus_tag="VE170_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HYJ16919.1"
                     /translation="MFTLKQFLKALFLPPMPWLLMLLAVLIFWQRRWARKLLFVTLLL
                     TLALHCGPLNYALRYQLESRYPPLLDPRKVGAYDAIVVLTSGSYPASGLIPFPSIDEH
                     MFRRLDEAWRLYRIQPKPIIISGGHVNPFTPAMDENKIARDFLIKWGVPKHHVIGEED
                     SRDTFENALASQKVLAKHGWKRYLLVTSALHMPRSMLVFGARVAEPIPAPGDFSVGKF
                     DFSPFSFFPSEGAAPKMFATLHEYIGWANYYWRLHYAGD"
BASE COUNT         1159 a         1591 c         1452 g          977 t
ORIGIN      
        1 atgaagtcta ccacatggtg cgcgcgacct tgcgcggctt gcagttcgtc agcgacgaag
       61 ccaatcgtga gcaggcgcgc gacgtcatga tgaaacagtg gcgtgtcagc gatcaaaagc
      121 tcgccgccga aatgctcggt tacctgaagc gcggcctggc caaagacgct gtgatcagtc
      181 cacaagcggt gcaatatttc gttgacctga cgcgcgagac ctctaacgtg gctcaaccga
      241 tccccggagc gcaggtcgtc gacttcagtt ttcttgaaaa agcgcgtaaa gacttggttc
      301 ggtagtttca taaccaggag gcgctatgct cgaattcgca ggccggcaga tccccactga
      361 gctaaaagag atcgtcgatc caaaacgcag cgtgctgcta gtctgggaca tgcagaacga
      421 tcaggcagga ggtgcattca acaaagaagc gcttatccgc actgcgccgc cgctgatcgc
      481 cgccgccgcc aacgccggcg tcaagaccgt ttacacgcgc cagacgccgt ttctctggag
      541 tgaagaggca gtcacctgga tacgccgcgc gatgatcaac gagaaggtcg atcatccgag
      601 caagctcaaa ccgcgacgtt tgaagggcag caccaactgg gagatcatga aacccttcct
      661 accggcgccc gaagacatcg tcattgacaa gcgccggccg agcatgttca tcggcaatga
      721 atttgaaacc atcatgcaca accgcggtgc cagcaccgtg gtcatgatcg ggtgcaccac
      781 cgacggcggc gtcgagatga ccgtgcgcga cggccacaac cgcggatttt caatggtcat
      841 cgtaggcgac tgcgtcggaa cttacaccga ggaaggccac cgcgcggcgc taaagcgcat
      901 ggagcgcttt gccgatgtgg tggagtcgaa ggacctgatc gagatctgga aaagttagat
      961 ggcgtcggtg catgaaatca aagatatcat cgagactttt cgcgcgggag acgaagagtc
     1021 ggcgtttttt actctgattg agttatcggg cgacgttttg ccggcattaa tcgagacttt
     1081 cagagatgaa aaaagttccg gtattcgcgc cttcttggtt aaagcagcct gggaacgccg
     1141 tgaagaggcg gtcctccctt ttctcagcga agtgctccac gaagcggaag aagaagcatg
     1201 gcaacaggcg ctggacgggc tggtagcgtt tgccacacca caatccctag agattcttca
     1261 tgccgcccgg tcaagatcgt ttactgaaaa gtccgcagcc aggagatttc ttttgtggct
     1321 ggacgaggcg accgaacaaa tcaagtctga actccgcgcc aaaaagtgat gcacggcagt
     1381 tttgccattt gcgattgctc cctcagtcga gttaatacta aggcaaattt cactcggcat
     1441 agcgccgtca ggagaccctc atgagcggaa ccaatggcaa gaaagcccta catgttgcga
     1501 cattttccgg ctggtaccga ttcgagcaga acggtaagca gttcacccag accaagcggg
     1561 ccttaagtta ctggacactc acctgtatga ccgtggacgc gaaagatccc aagacgatct
     1621 acgccggcac cgagcactcg ggacttttct tcactaaaga tgccggcgcc cactggaacc
     1681 gcgccgaacc gcaggtgccg aagatgttcc tctactcggc cctggccatc gacgggggcg
     1741 tcatggtcgg cacgatccca tccgccgtgt atcgcagcaa aaacggcggc tgggaggaac
     1801 tagagggcgt gcggctgcac agtgccggcg ccaattttcc gcctagtccg gagctgcagt
     1861 cgcgcacgcg ctacctcgcc tacgagccgg aaaaccagaa tcgcctgtac gccggcatcg
     1921 aagtcggcgg catggtgttg agcgacgacg gcggccgtgg ctgggagccg tgcaacgacg
     1981 gcctaaccga catggacatc cacgagatcc tcgcctccga gcagcacagc ggcatggttt
     2041 ttctcgcctg cggcgaagct tgcttccgca gtcccgatcg cgccggccac tgggaaaata
     2101 tttcgccaaa acatcatgac tacggcatct ccgtcgcaga agataaccgt ggcgtcctct
     2161 acgtcggcgc cgcgcgcggc cggcccaacc tctggctgcg cgaagaaggc gccatgtcgg
     2221 cgatcctgcg cagcagcgac aagggcaaga cttgggacac cgtcatcgac aatctaaccg
     2281 gcggcgtcat gcacctctgc ccgacgcccg atggtaatgg catgatcgcc ggcacctccg
     2341 atggcacctt gctgctggtc gacgacagcg gcgcgcgcga agtcgccagc ggcctgccgt
     2401 ttgttacctc ggtggaattg gcggcctgaa gcgaagccgc ccaaggcaat ccttcggcaa
     2461 ctggcgcagc atgctgcgcc cctacaggcg gaaattcata tgcgcatact gatcggcctc
     2521 tatctctgtc tctgtctgcc gtcgcatcta ctcgcccagg acgccaaact aatcgccgaa
     2581 ggcaggaaag acggcaaggt cgtgatttac ggctccatgg agaccgacat cttcgacggc
     2641 gttcagcaag gatttgaaaa gaaaacgggt atcaaggtcg actactggcg tgccgcgggc
     2701 gccaccgtgc tcgaacgcgc gtcgagtgag aagcgcgcca acaaaattgg ctacgacctg
     2761 gtgctcaaca atgccggacc gatggaaatc ctcctcagcg aaggcgcgct ggcgaaatat
     2821 gactcgccga tggcgaaaaa cttcgccgct gagtttcaac atccgcagct cggtccgagc
     2881 taccgcacct cgatcgtcgg catcgtctac aacaagagca ttatcagccc ggaaaaagcg
     2941 cccaagtcgc tggaggattt attgaagccg gagttccgcg gcaagctcgc ctttgccgat
     3001 ccgtcgcgcg gcgcggttgc cgtcatgtgg cccatgagcc tttacaaact catgggcaaa
     3061 gaccgcgccg ataaatacct gcgcgatctc ggcgccacca aaccggtgat cgtcgaaggc
     3121 gtcttgcccg ccgccgagcg ggtcaccacc ggcgagacgc cgatcgccat tacttatttg
     3181 aaatacgtca tcgtcttcgg ccaaaagggc gcgccgctgg actacgtcag gcaggacaag
     3241 ctgctcggcc acggccacgc catcgccatg agcagccgcg ccgcaaggcc caacgccgcc
     3301 aaggcgtttc tcgactactt ctacagtgac gaaagcctaa gagtgatggc caagtccggc
     3361 gagttcgtca cccgcaaagg tattcatccg ccgcttgccg acgctgacaa aatccaagtc
     3421 gtcgagatgg acgaacccga cgccaacatg atggccgaga aacgcaaaga gtacaggaaa
     3481 atatttcaat gaaactttac accttcttcc gcagctccgc ttcctaccgt gtgcgcatcg
     3541 ccctcaacct aaaagggttg agctgcgaac aagcgcctat ccatctgcgc cgcggcggtg
     3601 gcgagcaatt gatgccgtcg tacatggcgc tcaaccccca ggcgctggta ccggcgctgg
     3661 aggacaacag caggattctc actcaatccc tggcaataat cgaatatctc gacgagaccc
     3721 atcccacccc gccattgctg ccaaaggagc cggccgatag agccttggtg cgaagtatgg
     3781 caatggtcat cgcctgcgag gtccatccga tccagaatct gcgcgtgctg caatatgtca
     3841 aagcccgcta caaccagacc gacgaacagg tgaaccagtg ggcgcagcat tggatcgacc
     3901 tgggcctggc ggcgttacaa gaaatgatcg tcgcccaacc caaacgcggc aaattctgct
     3961 tcggcgcagt tcccaccctg gccgacattt gcctggtgcc ccagctcggc aacgcccggc
     4021 gctacggctg cgatctggcg aaatacccga cgattttaga aatcgaaacg aactgcaatg
     4081 ccatcccggc tttcgccagc gccgctccgg aaaatcagcc cgacgcggag tgatgagttg
     4141 gcgccagttg tcagtgccga gtttcgagtt gggagaacaa atcgaaccac atccgaatct
     4201 gacttccgac gctgacttct ggctactgcc ccaccatcca tgttcaccct caagcagttc
     4261 ttgaaagcac tttttctgcc gcccatgccc tggctgctca tgctgctagc ggtgctgatc
     4321 ttctggcagc gccgctgggc gcgcaagctg cttttcgtca ccttactgct caccttggcg
     4381 ctccactgcg gaccgcttaa ctacgcgctg cgctaccaat tagaatcgcg ttacccgccg
     4441 ctgctcgatc cgcgcaaagt cggcgcctac gacgccatcg tcgtgctcac cagcggcagc
     4501 tatccggcga gcggcttgat tccttttccc tccatcgacg agcatatgtt tcgccgcctc
     4561 gacgaggcct ggcggctcta tcgcatccaa ccgaagccga tcatcatctc cggcggccac
     4621 gtcaatccgt tcacgccggc gatggacgaa aacaagatcg cccgagactt tctcatcaag
     4681 tggggcgtcc ccaagcatca tgtcatcggc gaggaagatt cccgggacac ttttgagaat
     4741 gccctggcga gccagaaagt cctcgcgaag catggctgga agcgctatct tctggtcaca
     4801 tcggcgctgc atatgccgcg cagcatgctg gtgttcggcg cgcgggtggc cgaaccgatc
     4861 cccgcgcccg gagatttttc cgtcggcaaa tttgactttt cgcccttcag tttttttccc
     4921 agcgaaggcg ccgcgccgaa gatgttcgcc accctccacg agtatatcgg ctgggcgaat
     4981 tattactggc gtctgcacta tgccggcgac tgacggcgta agcgggcgcg tcgattgcaa
     5041 atccccggca gcgtgataga aactgcgctg gaattttgcg cgcagcgcga ccacgactcg
     5101 tatcgggaag cgccgaccaa tgatttcgat aagaaaccta ctgaagatct acaagtccgc
     5161 caacgacgcg gtgcatgcc
//
LOCUS       DASQKS010000608         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5384609, whole genome shotgun sequence.
ACCESSION   DASQKS010000608 DASQKS010000000
VERSION     DASQKS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5384609"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1603
                     /gene="metG"
                     /locus_tag="VE170_15585"
     CDS             71..1603
                     /gene="metG"
                     /locus_tag="VE170_15585"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYJ16920.1"
                     /translation="MSAVYLTSPLFYVNAEPHLGSTYTMVITDTLARYYRGKGAETFF
                     LTGTDEHGDKIAQVAAEAKVSEKEFTDRVSAAFRRIWDDCGITYDHFIRTTDAHHVKF
                     VQEVLQRVYDGGDIYFGKYGGLYCVGCERFYTEKELRDGKCPDHLIEPQLIEEENYFF
                     RMGKYQDQLREHIETHPDFIRPEGYRNEVLGMLREPIGDLCISRPKSRLTWGIGIPFD
                     ERYVTYVWFDALLNYVSALKIRGEEVFQKFWPMAEHFIGKDIVKPHGVFWPTMLMAAG
                     LPLFKHLNVHGFWTVEGQKMSKSLGNTVAPLAVKAEIGMDAFRYFLLRESVFGQDPDF
                     RHESLVSRYNTDLANNLGNFVSRALAMQQKYFAGIVQPLDGDWPAEDRELRDKFIQTE
                     KDIDGYINDLQFHRALESVMAALDHANRYIVQTAPFTMIKDADKQARVGAVLHQLLEV
                     VRTLARLLAPFLPDTVEELRALLALEPAALTKPWGQCFVAGHRINAPKVLFPRIDTAA
                     AK"
     gene            1618..2388
                     /locus_tag="VE170_15590"
     CDS             1618..2388
                     /locus_tag="VE170_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYJ16921.1"
                     /translation="MLIDSHAHIQGKEYAGEAEAVIARAHAAGVEAIIAVGGAGDMSS
                     NSEAVRLAHGFANVYATVGMHPHDAKDVGADELAVLQQLAEDPKVIAIGETGLDYYYS
                     HSPHDVQRRVFGQFIQMARQTELPIVVHERDAAQEAVELLRGEGGGDLRGVIHCFTGN
                     YQAATAYLDLGFYLSFTGIITFKNAEPLREVVRKVPLEKIFVETDSPYLTPVPHRGKR
                     NEPAYVRFVAEAIAKIKAIAVEEIAQVTSANVKQLFRI"
     gene            2443..2592
                     /locus_tag="VE170_15595"
                     /pseudo
     CDS             2443..2592
                     /locus_tag="VE170_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071864.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          657 a          879 c          852 g          692 t
ORIGIN      
        1 cgatccaccg caatctcaat cgccgcatgg ttttggagaa atacttgttt ggagttgtga
       61 agggggcgtc atgagcgccg tttatctcac ttcgccgttg ttctacgtca atgccgaacc
      121 ccacttgggc agcacgtaca cgatggtgat cacggacact ttggcgcgct actatcgggg
      181 caagggtgcc gagacatttt ttctcaccgg taccgacgag cacggcgaca agatcgccca
      241 ggtagcggcg gaagcgaaag tttctgaaaa agaatttacc gaccgcgtca gcgcggcgtt
      301 tcgccgcatc tgggacgatt gcggcatcac ctacgatcac ttcattcgta ccaccgatgc
      361 gcatcatgtg aaattcgtcc aggaggtctt gcagcgggtc tacgatggcg gcgacatata
      421 tttcggcaag tacggcgggc tttactgcgt cggttgcgag cgcttctata ccgagaagga
      481 attacgcgac ggtaaatgtc ccgatcatct catcgagccc caactgatcg aggaagagaa
      541 ttatttcttt cgcatgggta aatatcagga ccagctgcgc gagcacatcg aaacccatcc
      601 ggatttcatc cgcccggagg gctaccgcaa cgaagtgctc ggcatgctgc gcgagcccat
      661 cggcgaccta tgcatttcgc ggcccaagag ccggcttacc tggggtatcg gcattccgtt
      721 cgatgagcgt tacgttacct acgtctggtt cgacgccttg ctcaactatg tcagcgccct
      781 gaagatcagg ggcgaagaag tctttcaaaa attctggccg atggccgagc actttatcgg
      841 caaggatatc gtcaaacccc acggcgtatt ttggccgacc atgctgatgg cggcggggct
      901 gccgctattc aagcacctga acgttcacgg tttctggacc gtcgaaggcc agaagatgtc
      961 gaagagctta ggcaataccg tggcgccatt ggcagtgaaa gccgagatcg gcatggatgc
     1021 gttccgctat ttcttactgc gggaaagcgt cttcggccag gacccggatt tccgtcacga
     1081 gagtttggtt agccgctaca acacggacct tgccaacaat ctcggcaact tcgtcagccg
     1141 ggcgctagcc atgcagcaaa aatatttcgc cggtatcgtc cagccgctgg acggcgattg
     1201 gccggcggag gatcgggagc tgcgcgacaa gttcattcaa accgaaaagg atatcgatgg
     1261 ctacatcaac gacttgcagt tccatcgtgc cttggaatcg gtcatggcgg cgctcgatca
     1321 cgccaatcgc tatattgtcc agacggcgcc ttttaccatg atcaaggatg ccgataagca
     1381 ggcgcgcgtc ggtgcggtgc tgcaccaatt gctcgaagtg gtgcgcacgc tggcgcggct
     1441 actggcgcca ttcctaccgg atacggtgga agagttgcgc gccttgctgg cgcttgaacc
     1501 cgctgctttg actaagcctt ggggccagtg cttcgttgcc ggacaccgga tcaacgcgcc
     1561 gaaagttttg tttccgcgca tcgacaccgc cgccgcaaaa tagcaggtct gccaactatg
     1621 ctgatcgata gccacgccca tatccagggt aaggaatacg ccggcgaggc ggaagccgtg
     1681 atcgcccgag cccacgctgc cggggtcgag gcgattatcg ccgtcggcgg cgccggcgac
     1741 atgtcgagca acagcgaagc ggtgcggctg gcgcacgggt ttgccaacgt ttacgccact
     1801 gtcggcatgc atccccacga tgccaaggac gtcggcgccg atgaattggc agttttacaa
     1861 cagctcgccg aagatcccaa agtcatcgcc atcggcgaaa ccggcttgga ttactactac
     1921 agccattcgc cccacgacgt gcagcggcgt gtctttggcc aattcatcca gatggcgcgc
     1981 cagaccgagt tgccgatcgt cgtgcatgag cgcgacgctg cgcaggaggc cgtcgaatta
     2041 ttgcgcggcg aagggggcgg agacttgcgc ggtgtgattc attgcttcac gggtaattac
     2101 caagcggcaa cggcatatct tgacctgggg ttttatcttt ctttcaccgg catcattact
     2161 tttaaaaatg ccgagccgct gcgcgaagtg gtgcgcaaag tgccgctgga gaaaattttc
     2221 gtggaaaccg attcacccta tctcacgccg gtgccccacc gcggcaaacg caatgaaccc
     2281 gcctatgtgc gctttgttgc cgaggccatc gccaagatca aagccattgc agtcgaagag
     2341 attgctcaag taacctctgc taacgttaaa cagctgtttc gtatttgacc gcccggtttt
     2401 aaagccgccc aacaacagct ggaggacatt ttctccttca cctcgacgtt tcttggctaa
     2461 ttcatcgcgg tgtcggtgag cgaagttttc gaggcccaga ccttttccga cttcttccga
     2521 ttcccgatga tctttctttg cggtctcttt ttccccgtcg acgcgctacc gatgttattg
     2581 cgcccgctcg cgtaacctcc tgccgcgtat ccgttctttt tccaagtgcg aaattaaaaa
     2641 tcgttgttgg aataatgaac gtgcgcagca gccatgccaa tagagacagc ggagatccca
     2701 actcggcaat cgagcgtcaa ttctcatgga taggatcact ccagtggccg gacatcgaga
     2761 tgatctgtta tgggaatgac tgatctgaaa ctaaacggca taaggacagt gacgaccttt
     2821 cattggatct tcttcagcgg actgctgatg agcgcgatcg cgctcgccgg cagcgtcact
     2881 ctgttttctc agcgacgcag cgctgaaaaa attactgttg ccgctggtcg cgctggcagc
     2941 gggcactctg ctcggcggcg cgatcctcca catgataccc gccgccctcg aaaagagcgg
     3001 caaccaactg tcgatttatc tctggatttt gctcggtttt acgctcttcc tggcactcga
     3061 acagtttctc cactggcatc
//
LOCUS       DASQKS010000609         5667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2466755, whole genome shotgun sequence.
ACCESSION   DASQKS010000609 DASQKS010000000
VERSION     DASQKS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466755"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..891)
                     /locus_tag="VE170_15600"
     CDS             complement(70..891)
                     /locus_tag="VE170_15600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ16922.1"
                     /translation="MAAYQHRTIHVGDIKTHFLEAGSGPDLVLLHGGEYGASAEITWR
                     ANIDALAETFHIIAPDILGWGHTDKLYSFSDPAGLRIKHLQRFLEALGIGKAFFVGNS
                     AGGGLILRASVRQPAPLQIRKMVTICGNASVFKTTSQADLENYTPSLENMKKLIALLY
                     HDAKWQSDDNIRERYESSILPGAWETLSAARLRSPVHQARSTTEEFVKQLSQLTMPLL
                     LMSCAHDPLNHSDWDVHFQKIVPGSKIHRFKHSAHEPQIEETEEFNRVLTEFLLA"
     gene            complement(1059..1829)
                     /locus_tag="VE170_15605"
     CDS             complement(1059..1829)
                     /locus_tag="VE170_15605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HYJ16923.1"
                     /translation="MIQGLKDKVVIVTGGGHGIGRAYCHGFAEAGAKVIVADIDAPAA
                     EKVAGEVVQQFDGKALAAKVDVANEQSTKDMAKLTLDKFNCIDVLVNNAAIFSTIPMN
                     RGGIDTIDPAEWDRMMTVNLKGLFFCSRAVLPAMRKQKSGKIINISSGTALNGSAGRI
                     HYVTSKAGVIGFTRTLAREVGEDNINVNAIAPGSTLSEENPTEEVLKMRGARLADRAL
                     KRVQLPKDLVGTMLFLASPLSDFMTGQTVAVDGGVCFL"
     gene            1953..2675
                     /locus_tag="VE170_15610"
     CDS             1953..2675
                     /locus_tag="VE170_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16924.1"
                     /translation="MIRLFTFRAVALVLGIAVNCGSVGAAATTPASSDILPMFGQPKI
                     ARSDSQKQADEAFIRDNALRYKTRQAASSAFASQGWTAVRAKQLDAAMLRFNYAWLLN
                     PKNYSAFWGFGAVSSERGKLMEAIEHLETARELIDDPVQRSALLADLGNVHSEYASRL
                     SADKQLERAHHFVMANSRFTECVENDPKFASGWREWAISLYDQERYSEAWIKAKRARE
                     LKAEPFSPEFLKKLSAKMPEPK"
     gene            2697..3539
                     /locus_tag="VE170_15615"
     CDS             2697..3539
                     /locus_tag="VE170_15615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HYJ16925.1"
                     /translation="MSRFTIAQTIEQLDPEKDNQRIVFLSSRHDFPFDTTRALEFALF
                     RTFCVPSISALLDCTGEFAQRPQKRYDDTDIIVSELMEWGYDGERGQGALRRLNQLHG
                     RFAIANEDFLYVLSTFVYEPIRWNQRFGWRAMNEVERRGYFYFWREVGRRMNIKNIPP
                     DYGRFEQFNLDYEARHCRYSEANQRVGSATRELMVSWFPRFLAPLVRSAIYSLLDHRA
                     LAAFGFPPPIGAVRWLIIRALRLRGWCASYFPSGLRPRLRTEMVHRSYPAGYRIEDLG
                     PPRK"
     gene            3649..4449
                     /locus_tag="VE170_15620"
     CDS             3649..4449
                     /locus_tag="VE170_15620"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HYJ16926.1"
                     /translation="MAICLWAIDCPAQNSSDKEQLLTLWAGTMPIILAAPHGGREPIP
                     GVAPRRGIGVAQFVAERDSNTAELAEAVALKLRERLGATPFLVIARFERKYIDANREP
                     AAAYEMPQAKLYYDAYHRAIGEAVEAIRQKWGSGLLLDIHAQRAQADTIFRGTDNGRS
                     VSQLQRQFGQAALSGPNSVLGHLAANGYKILPNLTGQDRETRYTGGFTTRTYGSHRGT
                     KIDAIQLELGSDLRAKANLERTANDFALAIAVFTREYLLRGDGFDLRR"
     gene            complement(4543..5376)
                     /locus_tag="VE170_15625"
     CDS             complement(4543..5376)
                     /locus_tag="VE170_15625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ16927.1"
                     /translation="MAEIILKDEHDMLRPGLGRLKGKVAIVTGANSGIGRATSRLFAR
                     EGAKVVCCDIQETISPRIDSLIKEKEGGDALFAHCDVTKQEDLDRAVKTAVDTFGKID
                     ILYNNAGAGIRKKIHEHTDEEWNFVLNTNLNAMYRGAKAVMPVFIKQGSGNMVTTAST
                     FGLLASAEYPGYCATKAAIVNLTREMAIDYGPLGIRVNCVCPGAIETPRFRGFPPRPT
                     LGEGTTDEQRKRMSASNKALLRMGRPEEIAYGVLFLVSDEATFVTGHALVVDGGQTIA
                     V"
BASE COUNT         1166 a         1567 c         1637 g         1297 t
ORIGIN      
        1 cctagcaaac ttaagatcag accgatcaat ttgaaccggc gcatcggttg gcgtttcatt
       61 tcgtacccct caagcgagca aaaattcggt gagcacccgg ttgaactctt cggtctcctc
      121 gatctgcggt tcatgggccg aatgtttgaa acgatgaatt ttcgagccag gaacgatttt
      181 ttgaaagtgg acatcccaat cgctgtggtt gagcggatca tgcgcgcagg acatgagtag
      241 cagcggcatg gtcaactgcg acagttgttt gacgaattct tccgtggtcg agcgcgcctg
      301 gtgcaccggg ctgcgcaaac gcgccgccga tagggtttcc caggcgccgg gcaggatcga
      361 gctctcataa cgctcgcgaa tgttgtcatc gctctgccat ttcgcgtcgt gatagagcag
      421 cgcaattagc tttttcatgt tctccaatga cggcgtgtaa ttttccagat cggcttggga
      481 ggtggtcttg aatacgctgg cgttgccgca gatggtaacc attttacgga tctgcagcgg
      541 cgccggttgg cgcacggagg cgcgcaggat cagaccgccg cccgcggaat tgccgacgaa
      601 aaacgccttg ccgatgccca atgcctcgag aaaacgctgg agatgcttga tacgcaggcc
      661 ggcgggatcg ctgaagctgt acagtttgtc ggtatgtccc cagcctagta tatccggggc
      721 aattatgtga aaggtctccg ccagggcatc gatattggcg cgccaggtta tctccgccga
      781 ggcgccgtac tcaccgccgt gcagcaacac taaatccggt ccgctgccgg cttccaagaa
      841 gtgggtcttg atgtcgccga cgtgaatcgt gcggtgttga taagcggcca tcgatttagt
      901 ttcctagacc ccacaagaag ggggagttac tctagcagag acgcaaaggc gctaaggtaa
      961 taggaaaaac cgtttcgaac ttggcgtctt tgcgcctttg cgagagataa tccgatttct
     1021 gcttttgcgt tctttgcggt gaaatatctt cataccaatc ataaaaaaca cacgccgccg
     1081 tcgacggcga cggtctggcc ggtcatgaag tcgctcagcg gcgaggcgag aaatagcata
     1141 gtgccgacca gatctttcgg cagctgcacg cgcttcaatg cgcgatcggc caagcgggcg
     1201 ccgcgcattt tgagaacttc ctcggtggga ttctcttcgg acaaggtcga accgggggcg
     1261 atggcgttga cgttgatgtt atcttcgccg acttcgcggg ctaaagtacg ggtgaagccg
     1321 atgacgccgg ccttggaggt aacgtagtga atccgcccgg cgctgccgtt gagcgcggtg
     1381 ccggaggaaa tattgatgat cttgcccgac ttttgctttc gcatggccgg cagcacggcg
     1441 cggctgcaaa aaaacagtcc cttaagattc accgtcatca tgcggtccca ctccgccgga
     1501 tcgatggtat cgatgccgcc gcggttcatc gggatggtcg agaagatcgc cgcgttgttg
     1561 accagcacat cgatgcagtt gaatttatcc agggtcagct tggccatatc tttggtcgat
     1621 tgctcgttgg cgacgtcgac tttggccgcc agcgccttgc cgtcgaattg ctggaccact
     1681 tcaccggcga ctttttctgc ggccggcgcg tcgatgtcgg cgacgattac tttcgctccc
     1741 gcctcggcaa agccgtggca gtaagcgcgg ccgataccat ggcctccccc ggtgacgatc
     1801 accactttgt ctttcaatcc ttgaatcata aaattgctcc cataatttga tttgccgaca
     1861 aaatagcctt agatgttttt agctgtcaag gcgagtcatt gcgtctaatg aaaaaagcag
     1921 tgattatgcg ttggccaaag gcggtcgttt gaatgataag gttattcact tttagagccg
     1981 ttgcgctcgt gctaggtatc gcggtcaatt gcggctcggt tggcgccgcg gcgaccacgc
     2041 ccgccagttc cgatatcctg ccgatgttcg gccagccaaa aattgcccga agcgatagcc
     2101 agaagcaagc cgatgaagcg tttattcgcg ataacgcgtt gcgctacaaa acgcgccaag
     2161 cggctagcag cgctttcgct tcccagggct ggaccgcagt ccgagccaag caactcgacg
     2221 cggcgatgtt gcgcttcaat tatgcatggc tgctcaatcc aaaaaactat agcgccttct
     2281 ggggttttgg cgcggtatcg agcgagcgcg gcaaattgat ggaagcgatc gagcatctcg
     2341 agacagcgcg ggaactgatc gacgacccgg tgcaaagatc ggctttgctc gccgatctcg
     2401 gcaatgtcca ttcagaatat gcttcgcgcc ttagcgccga caagcagttg gagcgggccc
     2461 atcatttcgt catggccaat agccgattca cggaatgtgt ggaaaacgat cccaagtttg
     2521 cctccggttg gcgcgagtgg gcgatctcgt tatacgacca agaacgctac tcggaagcgt
     2581 ggatcaaggc caagcgcgcc cgcgaactca aggccgaacc gttttcgccg gagtttttga
     2641 aaaaactctc ggcaaagatg ccggaaccta aataacccta gcgaagtagc catcgcatgt
     2701 cgcgcttcac catcgcgcag accatcgagc agcttgatcc ggagaaggat aaccaacgga
     2761 ttgtttttct ctcatcgcgc catgactttc ccttcgatac cacgcgcgcg ctggagttcg
     2821 ctttgtttcg gacgttttgc gtgccgagta tatccgcctt gctggactgc accggcgagt
     2881 tcgcgcagcg gccgcaaaaa cgctacgacg acaccgacat catcgtcagc gagttgatgg
     2941 aatggggtta tgacggcgag cggggccagg gggcgctacg ccgcttaaat cagcttcacg
     3001 gcaggtttgc cattgccaac gaggattttc tttacgtgtt atcgacgttt gtttatgaac
     3061 cgattcgctg gaaccagcgc ttcggttggc gcgccatgaa cgaggttgaa cgacgcggct
     3121 atttttattt ttggcgcgaa gtcggccgcc gcatgaacat aaaaaatatt ccacctgact
     3181 acgggcgctt cgagcagttc aatctggact acgaggcgcg ccattgccgt tacagcgagg
     3241 cgaatcaacg ggtcggaagc gcgaccagag aattgatggt gagctggttc ccgcgattct
     3301 tggcgccgct ggtcagatcg gcaatttatt cgttgctcga tcaccgcgcg ctcgccgcct
     3361 ttggctttcc gccgccgatt ggggcggtgc gttggttgat aatcagagcg ttgaggctgc
     3421 gcggctggtg cgctagttat tttcccagcg gcctgcgccc gcgcttgagg acagagatgg
     3481 ttcaccgcag ctaccccgcc ggataccgca tcgaagactt gggtccgccg agaaagtaac
     3541 gacgacagac atgttgttgc cgcgcgcgcg agggggccaa agtcaatgaa cgcgattcgg
     3601 cggttggcat atctccgccg caacggccgg cgttgcatca gcttcatttt ggccatatgt
     3661 ctctgggcga tcgactgtcc ggcgcaaaat agctcggaca aggagcaact gttgacgcta
     3721 tgggccggta ctatgccgat tattctcgca gctccccacg gtggccggga gccgatccct
     3781 ggtgtcgccc cgcgccgggg tatcggtgtg gcgcaattcg tcgctgagcg cgacagcaat
     3841 accgccgaac tggctgaagc cgtcgcgctt aagctacgtg aacgattggg cgctacgccg
     3901 tttctcgtca tcgcccgttt tgaacgcaaa tacatcgacg ccaaccgcga gccggcggcg
     3961 gcctatgaaa tgccccaagc caaactctac tacgatgctt accatcgagc tattggcgag
     4021 gcggtcgagg cgatccgcca gaagtggggc agcggcttgt tgctcgacat tcacgcccaa
     4081 cgggcgcagg cggacacgat tttccgcggc accgataacg gtaggagtgt cagccaatta
     4141 caaaggcaat ttggccaagc ggcactgtcg ggaccgaata gcgtcctcgg ccatctggct
     4201 gccaacggtt ataaaatcct gccgaatttg actggccaag atcgagagac tcgttacacc
     4261 ggcggtttta cgacccgtac ctacggcagc catcgaggga caaagatcga cgccatccag
     4321 ttggagctgg gtagcgatct gcgcgccaag gcaaacctcg agcgcactgc caacgatttt
     4381 gccctggcta tcgcagtatt cacccgagag tatctgctcc gcggcgacgg attcgatctt
     4441 cgtcggtgag cggaaaaggc cgactatgcc gcaagcgttc tcagcgcccg tcggcttggc
     4501 gctagttctt gaaatctgcc tatggcgccg ggtttattac cactacaccg cgatagtttg
     4561 tcccccgtcc actaccagag catggccggt gacgaaagtt gcttcatcgg aaactaggaa
     4621 gagcacgccg taggcgatct cttccgggcg gcccatgcgg agcaaggctt tgttgctggc
     4681 gctcatcctt tttctttgct cgtcagtggt gccttcacca agggtcggtc gaggcggaaa
     4741 acctcgaaag cgcggcgtct cgatggcgcc ggggcagacg cagttgacgc gtattccaag
     4801 cgggccgtag tcgatggcca tctcccgggt gagattgacg atcgcggctt tggtggcgca
     4861 gtagccggga tattccgccg aggcgagcaa accaaaagtc gacgccgttg tcaccatgtt
     4921 gccgctgcct tgtttgatga acaccggcat caccgccttg gcgccgcggt acatggcatt
     4981 caagttggtg ttcaggacaa agttccattc ctcgtcggta tgctcatgga tttttttgcg
     5041 aataccggca ccggcattgt tataaaggat atcgatcttg ccgaaggtat cgaccgcagt
     5101 ctttacggcg cgatccaggt cttcctgctt ggtgacatcg caatgggcaa acagcgcgtc
     5161 tccgccttct ttttctttga tcaagctgtc gatgcgcggc gaaatggttt cttggatatc
     5221 gcagcacacg actttcgcgc cttcgcgggc gaacaggcgc gaggtggcgc ggccgatgcc
     5281 gctgttggct ccggtaacaa tcgcaacttt acctttgagc cgtcccagtc cgggacgtag
     5341 catatcgtgt tcgtctttga gaataatttc cgccattgct tgtgccccct agttcttgtt
     5401 tgtgaagtat tgaatgtcac ttatctcaga gcgctgcggg ttttcaaccg ggcggcaatg
     5461 agcaaatctg agtaagggaa gtgttggttc gaaattccat cactccacca ctgcagtact
     5521 ccaattccta aatcttgtta tttcttatac agctgcttga tgaatcccga cttttccagc
     5581 tcggcaacca ggctcatgtc gacgaaggtt ttcgggtcgg cgcttgccgc tttggggctc
     5641 ttcagcgcga ggtcgtcgag catggtt
//
LOCUS       DASQKS010000610        12180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5388028, whole genome shotgun sequence.
ACCESSION   DASQKS010000610 DASQKS010000000
VERSION     DASQKS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5388028"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1262)
                     /locus_tag="VE170_15630"
     CDS             complement(60..1262)
                     /locus_tag="VE170_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ16928.1"
                     /translation="MKKLLTLIYIGFFVWSCSAAPYTGRRQVILVSEGQETALGDDAY
                     KHVLRDSVIVRSPEAERIVRKVGERIAAAANKPDYKWEFTVINDPEMVNAFAVPGGKV
                     AAYTGIFAPARDEAGLAVVLGHEVAHALARHPAERMSQGMLLQLGGVGLGVALGKNPA
                     MANQVLQAYGIVGGVGIALPFGRSQETEADRIGLILMAKAGYDPRVAVDLWQRMESME
                     KKGSAKGTPPEFLSTHPGYETRVQNIRSFLPEALSYYQPSNARLETLPAPESLDTPAA
                     KSERELLKRIEAINRAVEQPNGERAVVEALAHELRLSPQAIVEERQQLQIGYGQYAAL
                     RGTAHLGRGSFSRVTDDYKRGRPWADVTDNNGTRLNELMVWFGDLIRTTNDMGQQLQR
                     QPSRPAIR"
     gene            complement(1376..2755)
                     /locus_tag="VE170_15635"
     CDS             complement(1376..2755)
                     /locus_tag="VE170_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ16929.1"
                     /translation="MDRLREKLTKIIDPARISANADYLDELSWDALSEGRFHPHHQPE
                     LAVPLCAVAPVSTDEIQELVRFANQENLSIVPFGGGSGLMGGALSVRSGLVLDLRRMN
                     RILAIDGESRSATVQAGVVLEAVDLALNPAGFILGHDPWTVPVATIGGAISTNSVGYR
                     AGIYGSMGEQVLGLEAVMPNGEVVRTRGVAKHSAGLNLNALLIGGEGCFGIITEATIR
                     IFPKPQARVLRGYGFASFEQGYGAIQEMFQRRLRPALLDYGDDAEKSGSSILYLVFEG
                     NAELTAVEEQQAAALCRAHGGKELPSRDAEQFWRDRHEIARRFARNRRQRREGGRDGI
                     YRDWIHVALPSSKVLAFRSAAQEIVERHGVHLQESGLWVQPELFSMRLSAEESMVTNA
                     QLALEEAIEELLRLVQQSGGSMEYTHGVGVKLAPLMVEEHGYGLQVMRQIKQALDPNN
                     VLNPGKMGL"
     gene            complement(2840..3259)
                     /locus_tag="VE170_15640"
     CDS             complement(2840..3259)
                     /locus_tag="VE170_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16930.1"
                     /translation="MKTTLGIIAIVALGMVLAGEVGAGPRRAGVAPRANSGVTSFAGG
                     GSNRVILQRQFSGGYPYYYNQPYYPQVLVVSPYGPSYILPPTVVVTSPYFCVMHNDGF
                     VSRVGMLDHLAGTHKIPLDAAASLCPDHGVSCVFPSY"
     gene            3415..4281
                     /locus_tag="VE170_15645"
     CDS             3415..4281
                     /locus_tag="VE170_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ16931.1"
                     /translation="MLAIIFGLFASFWFALSMVLINRGVLVIDYFRGLLTNLGINALF
                     LWLFIWLFVDRIELWLPANLIFVLVGIFVPGVARFFMFKGMERLGASITSCLTNSTPL
                     FATLFAVSFLQERPSLTNLVGTFSIVVGIVSLSWKGTSKTWRSRDLMFPLTAAFLFAA
                     RDNMVRFGLLKIYSPLVGATIAATTSFLTMSLLYLLFEEKKPLPATARKGYRLFAAAG
                     FLNFLSYAFAYTALSMERVSLISPLINGSSLFVLPLSALLLKDVEKLTPRKVGATVLV
                     IIGVFLISWEKM"
     gene            complement(4344..5066)
                     /locus_tag="VE170_15650"
     CDS             complement(4344..5066)
                     /locus_tag="VE170_15650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ16932.1"
                     /translation="MQYQTRKMSFGGGSVTPAVKFLLIANVGMFVVQTVIGLGTDFPL
                     SLYLGLVPQLVLNQFYLWQLFTYQFLHGGLFHLLFNMLALWMFGCDLERRWGMQFFLK
                     YYFVTVIGGAILNVVFLPGQLGPSIGASAGIYGLLLAYGLIYPERIVYFYFLFPIKMK
                     HFVWIIGAIAVYSSVTSGQSGIAHLAHLGGMVFGYIYLRGGINPWDQFKIYLDRRRLE
                     RLKKRFQVLPGGKDDDRGPTVH"
     gene            complement(5251..5952)
                     /locus_tag="VE170_15655"
     CDS             complement(5251..5952)
                     /locus_tag="VE170_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16933.1"
                     /translation="MLTVDAIHVYYGAIHALKGVSFSVAQGELVTLLGGNGAGKTTTL
                     KTLSGLLRPRSGAIKLHEKALSTVEPHEIVDRGVAHVPEGRKVFPRFTVLENLQIGGY
                     TRAKGAFGAELDFVYQMFPRLKERQKQYAGTLSGGEQQMLAIGRALMAKPKLLLLDEP
                     SMGLAPKIVEQILENIRDINKAGVTVLLVEQNAAMALAISHRGYVLETGAVILEGKAS
                     ELAGNDLVRQAYLGG"
     gene            complement(5939..6724)
                     /locus_tag="VE170_15660"
     CDS             complement(5939..6724)
                     /locus_tag="VE170_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ16934.1"
                     /translation="MALLELRDISKRFGGLQALSLVSMKIEEGMIASLIGPNGAGKTT
                     LFNTLTGIYRPDQGEIRFQERSLIGLKPESITGAGISRTFQNIRLFAHMSVLDNVLVG
                     MHTRLQSSLADIFLASKRFHAGEKAARRRAYELIELTGLGGRENEWAQQLSYGEQRRL
                     ELARALAAAPQLLLLDEPTAGMNASEAQSLMALFKDLMGRYVKAILLIEHNMRVVMGV
                     SDCVHVLDYGEKIAEGKPDAVRQDPRVIEAYLGTGDWLKNAHG"
     gene            complement(6932..8221)
                     /locus_tag="VE170_15665"
     CDS             complement(6932..8221)
                     /locus_tag="VE170_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYJ16935.1"
                     /translation="MSRSLAVPGLCLYIAASCALAYFFPQSVLAFLLVEASLFLLYYM
                     RVSARVKNLLGALVLLVLLPVLGFSNAYYFEIAIQIGIFVALALGLNIVVGFVGLLNL
                     GFVAFYAIGAYLWAVFGSPQANHFIAGGWFPLSPNWFFVFLLLSIAVGAATGALLGLP
                     VLRLRGDYLAIVTLGFAEVVRALVNNLDKPINLTNGPKGIAPISKPPLFFKPLLEFFG
                     VQVTDAALYPLYFYFLVLLMVFTIILVTQRLENSRVGRAWKAIREDEIAASAMGVPVV
                     RMKLLGFAVGASFASAVGVLFAAKQVFINPESFTFMESIGVLAMVIVGGMGSVPGAIL
                     GATGVTILNLQLLKGLSLWLNGLRQSGAVWSIPLVGDLALANLPAQFEPAKYERMIFG
                     LILIVMMIFRPQGILPPKEESGNNRGKFKIPDPQQIT"
     gene            complement(8218..9180)
                     /locus_tag="VE170_15670"
     CDS             complement(8218..9180)
                     /locus_tag="VE170_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYJ16936.1"
                     /translation="MALEILPQVLLDGIVLGFMYALIALGYTMVYGVLEFINFAHSEI
                     FVLGAFVGVEVLLMLQSAGLLETLHPAIIMLLVIVTGMTISGLAAMTLERIAYRPLMG
                     GPRLVALISAIGASFFLQDALRLFESLWRNSFYLNYPSLDFLDHNLPISASLVIPVKS
                     LLVIVTAILTLLGLHFFVNRTRMGMAIRAVAQDPDTASLMGVPVRRVIALTFFVGGAL
                     GGLAGVLFGIHYSLVNPYSGFVPGMKAFTAAVLGGIGNIPGAMVGGMLLGLLEAFAAS
                     YLSILTDGAFGAEYKDVVAFVVLIVILIFRPKGLLGEAVRESKA"
     gene            9314..10885
                     /locus_tag="VE170_15675"
     CDS             9314..10885
                     /locus_tag="VE170_15675"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HYJ16937.1"
                     /translation="MADAATTSAFAFQAGIKKFSKVLLIGGGVFAGLLAVLLVVPHFV
                     DLGLFKATYLPLIEDVLGRRVDVGEVHLRLVPTPSIQISKLRVADSQSIAGNTFFAAE
                     QLQLKLKFLPLVRGRFEVTELVLDKPVFNLLKQTDGSFNYSDLAGKKNPAAARREARK
                     KSEGSGKSVEAAPLALPSRLRIRDGQMNLFTNGQTSVNIQGIDISLQEISGDSPFPFR
                     ASFEYPGLKAVVLEGQLDYREDKALLELKNNRLKIYDLTLPVQGSISNLGTTPKVNLT
                     LNSEEVDAKPIFQILSPFGLAPSDTEISGPMALAMTIAGPSNALVTDIRGVFKKVYVK
                     GKRALKGNLTGEVNLRLPPGGGRVSQRLQGSGKLVARDGELTNVNLIKKIQRVTGMIG
                     LTPDQQREATTFKTMEGDFTIGSGVADFSRLYLVNPQMEVTGNGTMTLEQPALDILLN
                     TTLSPQASARTGGGRSVRFLKDHQGRIMVPLRVAGPVENPSVNLNVAKLSESGLPKTV
                     EKGFSSFFKHLFRGR"
     gene            complement(11038..>12180)
                     /locus_tag="VE170_15680"
     CDS             complement(11038..>12180)
                     /locus_tag="VE170_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16938.1"
                     /translation="DATAIGNFSTGKIASITDTSSTSISKGLMFVVERGSVMMDGALF
                     NFEHAAQIPGNRVVLLLIAVITILASSMSGSSVAMRFIETQVSSVRLTQVAVVMILTG
                     ILANEFIVAGFLSQDGALELSTLHKLRWLQSVLVMSGLVLMAVRRTLVASIKRLPVYM
                     GESNLIKQHSEKLRLLAVSLVIPWILLLLLVENTNHLVRLWWLWPLQVVFLSLPATYL
                     SSRSGSTRLSAGIGCFILALLVIANPLLLTRLESWLANGWSGDDAEVIKVVDFTAKRI
                     ILDGKDHAAIGYDIPMSLFYAGFNAADRRYKVGANLDLLFKYRHGIINTDRCAEGFSS
                     GDEYRIVQPSDGAVAELKADDRFDIPYDPRMQIVHSFKSYQLLQRH"
BASE COUNT         2827 a         3635 c         3280 g         2438 t
ORIGIN      
        1 cgcgaaacca ccgaaaggct cgatcgcccc gcttcgtcgg ttgtcgctct accgtgagcc
       61 tagcggatcg ccggccgtga tggttgcctt tgcaactgct gtcccatgtc attggtcgtg
      121 cgaatcaggt cgccaaacca aaccatcagt tcgttaagcc tggtcccgtt attatcggtc
      181 acatcagccc atggccggcc ccgcttgtag tcgtcggtga cgcggctgaa cgaaccgcgg
      241 ccgagatgag cggtcccgcg tagggcggcg tattggccgt aaccgatttg cagctgttgg
      301 cgttcctcaa cgatcgcctg cggactcaac cgcagctcgt gcgccagggc ctcaaccacc
      361 gcgcgctcgc catttggctg ctcgacggcg cgattgatcg cctcgatccg cttgagcaat
      421 tcacgctcgg attttgccgc cggcgtgtcc aaggattccg gcgccggcag ggtttcaagc
      481 cgcgcattgc tgggctgata gtaggacaag gcttccggca gaaacgaacg aatattctgc
      541 acccgggttt cgtaaccagg atgggtcgag agaaactccg gcggcgtccc cttggcgcta
      601 cccttctttt ccatcgactc catgcgttgc cacagatcca cggcaacgcg cgggtcgtaa
      661 ccggccttgg ccatgaggat taaaccgatg cgatccgctt cggtttcctg cgagcggccg
      721 aacggcaacg caataccaac accgccgacg ataccgtagg cctgcaaaac ttgattggcc
      781 attgccggat ttttgcccag cgcaacgccg aggccgacgc cgcccagttg cagcagcatg
      841 ccctgactca tgcgctcagc cggatgacgc gccaacgcat gggcgacttc atggccaagt
      901 accaccgcca aaccggcctc atcccgcgcc ggcgcgaaaa ttccggtata ggccgccacc
      961 ttgccgccgg gtacagcaaa ggcgttgacc atctccggat cgttgatcac cgtgaactcc
     1021 cacttgtaat cgggcttatt cgccgcagcc gcgatgcgct cgcccacttt gcgcacgatc
     1081 cgctcggctt ccgggctgcg cacgatcacg ctgtcccgta gcacatgttt gtaggcatcg
     1141 tcaccgagcg cggtttcctg tccctcggaa accaagatca cctgccggcg accggtatac
     1201 ggcgccgcgg aacagctcca aacgaaaaat ccgatgtaaa tcaacgtgag caattttttc
     1261 atagacaccc cgaggcacgc gaataccagg caaaaaaata acgtttagca tcgaccggcg
     1321 caaggaatcc ttggcgctga gacttagacg tatgaacgag agtaggaaca accggctata
     1381 agcccatttt tcccggattg agcacattgt tggggtcgag ggcctgcttg atctggcgca
     1441 tcacctgcaa gccatagcca tgctcctcaa ccatcagcgg cgccagcttg acgccgacgc
     1501 cgtgggtgta ctccatcgac ccgccgcttt gttgaaccaa gcgcaaaagc tcttcgatgg
     1561 cctcctccaa agcaagctgg gcgttggtca ccatgctctc ttcggcgctg agacgcatgg
     1621 agaaaagttc cggttgcacc cacaaaccac tttcttgcaa atgcacgcca tggcgctcga
     1681 caatctcctg cgcggcgctg cggaaagcca agaccttcga actcggcaaa gcgacgtgaa
     1741 tccaatcgcg ataaatccca tcccggccgc cctcgcgccg ttgacggcga ttgcgggcga
     1801 atcgtctggc gatttcatgg cggtcgcgcc aaaactgctc ggcatctctg ctcggcaatt
     1861 ccttaccgcc gtgagcacga caaagggccg cggcctgctg ctcttcgact gcggttaact
     1921 cagcattgcc ctcgaagacc aagtaaagaa tcgagctacc agatttttca gcatcgtcgc
     1981 cataatcgag cagcgccggc cgcagccggc gctgaaacat ttcctgtatc gccccgtagc
     2041 cctgttcgaa cgatgcgaaa ccgtagccgc gcaacaccct cgcctggggt ttagggaaaa
     2101 tgcgtatcgt cgcctcggtg atgatgccga aacaaccctc gccaccgatc agcaaagcat
     2161 tgagattcag ccccgccgag tgttttgcca ctccccttgt gcgcacgacc tcgccgtttg
     2221 gcatcaccgc ttccaagccc agaacctgct cgcccatgga gccgtaaata ccagcgcggt
     2281 agcccacgct attggtcgag atcgcgccgc caatagtcgc taccggcacc gtccagggat
     2341 catgccctag gataaagccg gcagggttca atgccagatc aacagcttcg agcaccaccc
     2401 ccgcctggac ggtggcagaa cgagactcgc catcgatggc gagtatacgg ttcatccgcc
     2461 gcaggtcgag aaccaagcca gagcgcaccg atagagcgcc gcccatcaaa cccgagccgc
     2521 ccccgaaagg aactattgac aagttttctt gattggcgaa acgaaccagc tcttgaatct
     2581 cgtcggtcga cacaggcgct acggcgcata aaggcaccgc cagttccggc tgatgatgcg
     2641 gatggaaacg gccttcactc agcgcatccc acgaaagctc gtctaaataa tcagcatttg
     2701 ccgagatccg cgccgggtcg attattttcg tgagtttttc gcgcagtcgg tccatgagtt
     2761 tggcggcggc tccgcctaga caaactatat cgaaatcgcc accgaggatc gacagctaac
     2821 acagcggccg atcgtgcagc taataggacg gaaagacgca gctcacgccg tgatcgggac
     2881 acaggcttgc cgccgcgtcg agaggaatct tgtgggtgcc ggcaagatga tcgagcatgc
     2941 cgacccggct gacgaatcca tcgttgtgca tcacgcagaa atacggcgat gtgaccacca
     3001 ccgttggcgg caggatatag gagggcccgt agggcgagac caccagcacc tgaggataat
     3061 agggttgatt ataatagtag ggatagccgc cagaaaattg gcgctgcagg atgactcgat
     3121 tagaaccgcc gccggcaaaa gacgtcacac cagagttggc gcgcggggcc acccccgccc
     3181 ggcgcggtcc ggctcccacc tcgccggcga ggaccatccc gagggcaact atcgcaatta
     3241 tacctagcgt tgttttcatg cctgcccttc cttacgaaat cctcggatcc ggcgtggtaa
     3301 tgcaatatat tagacgtttg agactgtcaa atcgtagcgg cgcgcgactg tctgggaacc
     3361 ggattgccga attgcacccg ctctcacctc aggataaggt cgcgagaatt catcatgctc
     3421 gcgattatct tcggtttgtt cgcctccttc tggttcgccc tatcgatggt cttgatcaac
     3481 cgcggcgttt tggtcatcga ctactttcgc ggcctgctca ccaacctggg catcaacgcg
     3541 ctgtttcttt ggctttttat ctggctgttt gtcgaccgca tcgagctttg gcttcccgcc
     3601 aatctgatat tcgttctcgt cggcatcttc gttcccggcg tcgcgcgctt tttcatgttc
     3661 aagggcatgg agcggctggg tgcatcgatc acatcctgtt tgaccaacag cacgccgctg
     3721 ttcgccaccc tgttcgccgt gtcgtttctg caagagcggc cgagcctgac caatctggta
     3781 ggcacgtttt ccatcgtcgt tggcatcgtc tcactcagct ggaaaggcac gagcaagacc
     3841 tggcgcagcc gcgacctgat gtttccgctc accgccgcct ttctcttcgc cgcccgcgac
     3901 aacatggtcc gtttcggttt gctaaaaatc tattcccctc tggtcggcgc gaccattgcc
     3961 gccactacgt cgttcctcac catgtcgctc ctgtacttgc tattcgaaga aaaaaagccg
     4021 ctgccagcca cagcccgcaa aggctaccgg ctcttcgccg ctgccggttt tctcaatttt
     4081 ctctcctacg cctttgccta taccgccttg agcatggaac gggtctcgtt gatctcaccg
     4141 ttgatcaacg gctcgtcgct cttcgtcctg ccgctgtcgg cgctgcttct gaaagacgtc
     4201 gagaaactca cgccgagaaa ggtcggcgcg accgtgctgg tgattatcgg cgtgtttttg
     4261 atttcgtggg agaagatgta gtgagacgag tccgggtcaa aggctcgagg cggcgaagga
     4321 atcgagtcta gggccgggtt atttcaatgc accgtcggcc cgcggtcgtc gtctttgccg
     4381 ccggggagga cttggaagcg tttttttaac cgttcgaggc ggcggcggtc gaggtaaatt
     4441 ttgaactggt cccatggatt gataccgccg cgcaggtaga tataaccgaa taccatgccg
     4501 ccaagatggg cgaggtgagc gatgccgctt tgacctgaag taaccgagga atacaccgcg
     4561 atggcgccga tgatccagac gaagtgtttc atcttgatcg gaaacagaaa ataaaaataa
     4621 acgatgcgct ccggatagat caaaccgtag gcgagcaaca gtccgtagat accggctgat
     4681 gcgccgatgc tagggccgag ctgacctggc aagaacacga catttaaaat agcgccgccg
     4741 ataaccgtga caaaataata cttgagaaaa aactgcatac cccagcgccg ttccagatcg
     4801 cagccgaaca tccacaacgc cagcatgttg aacagcagat gaaacagacc gccatggaga
     4861 aactggtagg taaagagctg ccagaggtaa aactgattga gaaccagctg cggcaccaga
     4921 ccgaggtaaa ggctcagcgg gaagtcggtg ccaaggccaa tgacggtttg cacgacaaac
     4981 atgccgacgt tggcgatcag cagaaacttc accgccggcg tcacactgcc gccgccgaaa
     5041 ctcatctttc gggtctgata ctgcatggac tctaagtcta acggttattg cgcccggtta
     5101 aaagttcgcg ggtgggtttt aaacgctccc gataaagcca aattcttctt tgcgccattt
     5161 gcggtctatg ggctaactcc ccgagtccga aacttctact ttgtgatctt tgtgctcttt
     5221 gtggttaaat ccttttcctc ccataagcct tcacccgccc aagtacgcct ggcgcactag
     5281 atcattgccc gccaactcgc tcgccttgcc ttcgagaatc accgcgccgg tctcgagcac
     5341 gtagccgcgg tgcgagatcg ccagcgccat cgcggcgttt tgctcgacga gcaaaacggt
     5401 gacacccgcc ttattgatgt ctcggatgtt ttcgagaatt tgctcgacga ttttcggcgc
     5461 cagtcccatg gacggttcgt cgagtagcag cagcttgggt ttggccatca aggcgcggcc
     5521 gatagcgagc atctgctgct cgccgccgga cagcgtgccg gcgtattgct tttgccgttc
     5581 tttcaaacgc gggaacatct gatagacgaa gtccaactcg gcaccgaatg cgcctttcgc
     5641 tcgggtgtag ccgccgatct ggagattttc cagcaccgtg aagcggggaa aaacctttcg
     5701 cccctcgggc acatgggcga cgccgcggtc gacgatctcg tggggttcca cggtgctgag
     5761 cgctttttcg tgcagcttga tcgcgccgct acgaggccgg agcagaccgg agagggtctt
     5821 caaagttgtc gtcttgcctg cgccgttacc gccgagcaag gtaaccagtt cgccttgggc
     5881 gacgctaaat gacacgcctt tgagcgcgtg aatcgcgccg taatagacgt ggatagcgtc
     5941 aaccgtgagc attcttcaac cagtcgccgg tgccgagata agcttcgatg acgcgcggat
     6001 cctggcgcac cgcgtcgggt ttgccctcgg caattttctc gccgtagtcg agcacgtgca
     6061 cgcaatccga cacgcccatg accacgcgca tattatgttc gatcaagaga atcgctttga
     6121 cgtagcggcc catgagatct ttgaacagcg ccatcaacga ctgcgcctcg ctggcgttca
     6181 taccggcggt gggctcgtcg agcagaagca gctgcggtgc ggccgccaat gcgcgggcta
     6241 attccaggcg gcgctgctcg ccataggaga gttgctgcgc ccattcgttt tcgcgcccgc
     6301 ccagcccggt aagctcgatc aactcatagg cgcgccggcg cgccgccttt tcgccggcat
     6361 gaaatctttt cgacgccaga aaaatgtccg ccaagctcga ttgcagacgc gtatgcatgc
     6421 cgaccaagac gttgtcgagc acactcatat gcgcgaacag cctaatattt tgaaacgtcc
     6481 ggctgatgcc ggcgccggta atcgattcgg gcttcaaacc gattaacgag cgctcttgga
     6541 aacgaatctc accttggtcc ggccggtaga tgccggttag agtattgaac agcgtcgtct
     6601 tgccggcgcc gttgggaccg atgagcgatg caatcattcc ctcttcgatc ttcatgctga
     6661 ccagcgacag cgcttgcagc ccgccaaagc gttttgagat gtcgcggagt tcgagcaatg
     6721 ccatcttcag ttcctgctca tcactcttcg tcgctggcta gggtatggaa gtatctcatg
     6781 caaagaggtc agccatcctt caagcaaaat cgcacgagta tcgaaaccgg cgtcgtggag
     6841 ccaaaacgaa cctcaaccaa gttagccaat tttagagcag tgccaaaacc agcaatccgc
     6901 gccagggttg tatggaacat tgtgtctttg gtcatgtgat ttgttgcgga tctggaattt
     6961 tgaattttcc tcggttatta cccgattcct ccttgggcgg cagaatcccc tgcggacgaa
     7021 agatcatcat aacgatcaaa atcaggccga agatcatgcg ctcgtatttc gccggctcga
     7081 actgcgccgg caggttagcc agcgccaaat cgccgaccag cggaatcgac cacaccgcgc
     7141 cggattggcg caagccgttg agccagagcg atagtccttt gagcaattgc aaattgagta
     7201 tcgtcacgcc ggtggcgccg aggatcgcac cgggcaccga gcccatgcca cccacaataa
     7261 ccatagccag cacgccgatg gattccatga aggtaaaact ctcgggattg ataaatacct
     7321 gcttggcggc gaacaacacg ccgacggcgg aagcgaacga ggcgccgacg gcgaagccca
     7381 ataatttcat gcgcacgacg ggaactccca tggcggaggc ggcgatctcg tcctctcgaa
     7441 tcgctttcca ggcccgcccc acccgtgagt tctccaatct ttgagtaacg aggatgatgg
     7501 taaacaccat gagcaggacg agaaagtaga aatacagcgg ataaagcgcc gcgtcggtta
     7561 cttgcacgcc gaagaactcc agcagcggct taaaaaacag cggcggcttg gagatcggcg
     7621 cgatgccctt gggtccgttg gtcagattga tcggcttgtc taaattgttg accagggcgc
     7681 gcaccacctc ggcaaagccg agggtgacga tggcgagata atcgccgcgc agccggagca
     7741 ccggcagtcc taacaacgca ccggtggcgg cgccgacagc aatgctgagc agcagaaaaa
     7801 caaaaaacca gttgggcgat aacggaaacc agccgccggc gatgaaatga tttgcctggg
     7861 gagatccgaa caccgcccac aggtaggcgc cgatggcata gaacgcaacg aaaccgagat
     7921 tgagcaaacc gacgaagccg acgacgatgt tgagtcccag ggcgagggca acaaaaatgc
     7981 cgatctgaat cgcgatctcg aaataatagg cattcgagaa acccaagacc ggcagcagca
     8041 ccaacaacac cagcgcgcca agcagatttt tcacccgggc gctcacccgc atgtaataga
     8101 gcaaaaacaa tgatgcctcg accagcaaaa acgccagcac ggattgagga aagaagtaag
     8161 cgagcgcgca actcgccgcg atataaagac aaagaccggg caccgccagt gaccggctca
     8221 tgccttgctc tcccgcaccg cctcaccgag cagacccttg ggtcgaaaaa tcaagatcac
     8281 gatcaacacg acgaacgcca ccacgtcttt gtactcagcg ccaaaggcgc cgtcggtgag
     8341 gatcgaaaga tacgacgccg cgaatgcttc caacaaaccg agcagcatgc cgccgaccat
     8401 ggcaccggga atgttaccga tgccgccgag cacggcggcg gtgaatgcct tcattcccgg
     8461 gacaaagccg gagtaggggt tgaccagact gtaatgaatg ccgaacaaca cccccgccaa
     8521 gccgccgagc gcaccgccaa cgaaaaaggt caacgcgatg acccgcctga ccggcactcc
     8581 catgagcgat gccgtgtcgg gatcttgcgc gacagcgcgg atcgccatgc ccatgcgcgt
     8641 gcggttgacg aagaaatgca acccaagcag cgtcagaatc gccgtaacga tcaccaggag
     8701 cgacttcacc ggtatgacca acgacgccga tatcggcaaa ttatgatcga ggaagtccag
     8761 actcgggtag ttgagataaa acgagttgcg ccacaaactt tcgaacaaac gcagggcgtc
     8821 ctggagaaag aacgaggcgc cgatggccga gatcagggcg accaatctcg gcccgcccat
     8881 cagcggccgg taggcgatgc gttcgagcgt catcgccgcc aatccgctga tcgtcatgcc
     8941 ggtgacgatg acgagcagca tgattatggc tggatggagg gtctccaaga ggccggcgct
     9001 ctgcagcatt aacagcacct cgacgccgac gaaggcgccg aggacgaata tctcgctatg
     9061 ggcgaaattg ataaactcca acaccccata gaccatggtg tagcccaggg cgatgagcgc
     9121 gtacatgaaa cccaacacga tgccgtcgag caaaacctgg gggagaattt ccagcgcgat
     9181 ttccaagcag ttgtgaaccc ttcagggacc gcatgctaac aaatagttgt tggcctggct
     9241 acccgcccgc gccatctaat tattgggatt gcctggtgat tgacgtataa tagcgctcat
     9301 taggccctca accatggcag acgcggcaac cacatcggca ttcgctttcc aagccggcat
     9361 caagaaattt agcaaagtcc tgctcattgg cggcggcgta ttcgccggac tactcgccgt
     9421 tctactcgtc gtaccgcact ttgtcgatct gggtctgttt aaggcaacct atttgccgct
     9481 catagaggac gttcttggcc ggcgcgtcga tgtcggcgaa gtgcacttgc gcctggtgcc
     9541 gacgccgtcg atccaaattt ccaagctgcg cgtcgccgat agccagtcga tcgccggcaa
     9601 tactttcttc gcggccgagc aactgcaact caaactgaag tttctgccac tagtgcgggg
     9661 ccgcttcgaa gtcaccgagc tggtgctcga caagccggtt ttcaacctgt tgaagcaaac
     9721 ggacggcagt ttcaactact ccgacctcgc cggtaaaaaa aacccggcgg cggcgcgccg
     9781 ggaggcaaga aagaaatctg aaggcagcgg caaaagcgtc gaagccgcac cgctggcgct
     9841 gcccagccgc ctgcgcatcc gcgatggcca aatgaacctc ttcaccaatg gccaaacctc
     9901 ggtcaatatc cagggaatcg acatatcgct gcaagaaatc tccggcgact cgccatttcc
     9961 gtttcgcgcc tcgttcgagt accccggact caaggctgtc gtgctcgaag ggcagctgga
    10021 ttaccgggaa gacaaagcgc tgctggagct gaagaataac cgcttgaaga tctatgacct
    10081 gacacttccg gtccaaggca gcataagcaa cctgggaacg acgccgaaag ttaatctgac
    10141 cttgaacagc gaagaggtcg acgccaagcc gattttccag atcctttcac ccttcggtct
    10201 cgctcccagc gatacggaaa tctccgggcc catggcgctc gccatgacaa ttgccggtcc
    10261 gtcgaatgcg ctggtcaccg acatacgggg cgtttttaaa aaggtttacg ttaaaggcaa
    10321 gcgcgctttg aaaggcaacc tgaccggcga ggtcaacctc cggctaccgc ccggcggcgg
    10381 ccgggtgagc caacgcttgc aaggcagcgg caaactggtc gcccgcgacg gcgagctgac
    10441 caatgtcaat ctgatcaaga agattcagcg cgtcaccggc atgatcggcc tgacgccgga
    10501 ccagcagcgc gaagcgacga cttttaaaac catggagggc gacttcacca tcggctccgg
    10561 cgtcgccgac ttctcgcgcc tctatctcgt caatccacaa atggaagtca ccggcaatgg
    10621 caccatgact ctagagcaac cggcactcga catcctgctt aacaccacgt tgtcgccgca
    10681 agcctcggcg cgcaccgggg gcggccgcag cgtgagattc ttaaaagacc accaaggccg
    10741 tatcatggtc cccctcaggg tcgccggacc ggtggaaaac ccgtcggtga atctcaacgt
    10801 cgcgaaacta agcgaatccg gtttgccaaa aactgtcgaa aagggattct cgtcattttt
    10861 caagcatctc tttcgtggcc ggtgaccgat cccgctcgcc gccgctgctg cagctactac
    10921 tgccgcaatt acgtgttttg ctctcagccg ccaagtagaa acaccttttt gcagtgactg
    10981 ccgcggacga taacgtggag aagtaaaccg ggagggaaaa gcagtggatt cgaataatca
    11041 atgacgttgg agcagctgat atgacttgaa actatgcacg atttgcattc tcggatcata
    11101 gggaatgtca aaacgatcat ccgctttaag ttctgcgact gcaccgtcgc tcggttgcac
    11161 tatccgatat tcatcgccgg atgaaaaccc ctcggcacac ctgtctgtgt tgataatccc
    11221 gtgacggtat ttgaacagca gatccaggtt agcgccaacc ttataacggc ggtcggcggc
    11281 attgaaaccg gcataaaaca gactcatcgg aatatcataa ccaatggcgg cgtgatcttt
    11341 gccgtccaag ataattctct tggccgtgaa atcgactact tttattacct ctgcgtcatc
    11401 tccagaccag ccgtttgcga gccaagactc caaacgggtc agcagcagag ggttggcgat
    11461 taccagcaag gcgagaataa agcagcctat cccggcgctt aaccgagtgg atcccgaacg
    11521 cgatgacaag taggttgcag gcaaagaaag aaataccacc tgaagcggcc atagccacca
    11581 cagtcttacc agatgattag tattttcaac caacaacaga agcaatatcc agggaataac
    11641 tagactcacg gccagcaatc tcaatttttc tgaatgctgt tttataagat tgctttcccc
    11701 catataaacc ggcagccgtt tgatactggc gacaagcgtt cttcttactg ccatcaagac
    11761 aaggccgctc atcacaagta ccgactgtaa ccatcgcagc ttatgtaagg ttgataattc
    11821 cagagctcca tcctggctca gaaaaccggc cactataaat tcattcgcca gtatccccgt
    11881 cagaatcatg acgacggcaa cttgcgtcaa ccggacactt gaaacttgag tttcaataaa
    11941 tctcattgcg accgacgatc cggacatgct gctcgccaga atagtgatta cggctatgag
    12001 cagtagaacg acacgatttc cgggaatttg cgcagcatgc tcaaagttga ataatgcgcc
    12061 gtccatcatg acggatcctc gctcaaccac gaacatcaaa cccttagaaa tacttgtcga
    12121 agacgtatcc gtgatagacg cgatttttcc ggttgaaaaa ttacctatcg cggtggcatc
//
LOCUS       DASQKS010000611         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5388672, whole genome shotgun sequence.
ACCESSION   DASQKS010000611 DASQKS010000000
VERSION     DASQKS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5388672"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..736
                     /locus_tag="VE170_15685"
     CDS             446..736
                     /locus_tag="VE170_15685"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HYJ16939.1"
                     /translation="MISQIRIYTINKGEMDNFLKHFKDDIVALHQKIGVPIVGSWVNR
                     PQNEFIWIRSYKDKAEMEAKGKEFQAAVATAGVKLGSNVAKMEVREVEAAFA"
     gene            complement(860..1168)
                     /locus_tag="VE170_15690"
     CDS             complement(860..1168)
                     /locus_tag="VE170_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16940.1"
                     /translation="MNLIQLLLPLHDNDKRLFSRDEFDRVRDELAKRFGGITAFRRSP
                     AEGVWREGQEDVSRDEVVIFEVMTERLNRKWWSGYRKDLEKRFRQEELVIRATQVELL
                     "
     gene            1436..2449
                     /locus_tag="VE170_15695"
     CDS             1436..2449
                     /locus_tag="VE170_15695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16941.1"
                     /translation="MRFGLEFGSYPSELPAGKVCEQVVERAQVAQRNNFEALFVAQHF
                     LTGPEAAILQSLPLLSYLAGRVPGMYVGTSIFLLPLHHPVMVAEYMSTLDNLSGGKLL
                     FGVGQGYRESEFNSFGIDLRERRGRLVESLEIIRRLWTGDAVTFHGKYFHLDNVMMGP
                     RPVQVPGPPILLGADSVRTVAKVPEVADHWIASRRHSKGFLREALPVYKAALEKQGRP
                     YKGLFIFRDLCIADSTKEAEERIREGYERRYLRYQQWGQPGERYDLKFDELKRDRLIL
                     GCPAEVVEQVMSYYEEFNAEVMWFMVDWPGVDPRFALETIERFGAEVIPAVNRRTRPC
                     PLP"
     gene            2673..3911
                     /locus_tag="VE170_15700"
     CDS             2673..3911
                     /locus_tag="VE170_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ16942.1"
                     /translation="MQQEVLDQVDRDRIVEMASNLANIVSITGDEENVAKYLGSELET
                     LGMKVEYQYVEDGRPNVIGTLKGSGGGATLMFNAHMDHFDAPQETVVDEDRIYGRGLV
                     NMKAAFPCYIEAVAALQKAGAKLKGDLIIAGVVGEIEKAQIGRFHGKEYRGAGVGARY
                     LMDHGVMADMCIIGEPTGLQLQIGNSGLLWARVSVDGKATKFVLAACKVAQAIQDWEK
                     EYQRKNPHKFMLPTVQIGAIDGGNAFKPGTRPTTDIFVIVKMLPRAVPLAIKRDLEAV
                     CERVRKREPDILDIRVELYLTTPGYEISKSEPLVKVMESAHRTVFNGPVPYAKPIRYG
                     ITSDGSRIAQYGVPAITYGPGFGTHLVDPTETKAGEEWDSPTGVRRGVGITNMVNCTK
                     VYAMAALDICNRKKEEVAKS"
BASE COUNT          949 a         1139 c         1202 g          906 t
ORIGIN      
        1 cacgggctga ctcgatcgac gtgtaggcag ctgattcgat cgaggttgga gtgagctccc
       61 aaacgaaccg aacgtgaact tgctgcttcg cggcaacggg gcagtcgcgc ttactcaccg
      121 cggccaccgg gaccgagagc cggtgaagta tgtatttcac ccaccaatca acggtccgtt
      181 gcgcatttta ttatgcagct tcagatgaag cgctagtccg gacaaaccgg gtccgcgcaa
      241 caagttcatt ctgctgcacg tgaaaccttc gtctgtatct tgcaatggtg atcatccgag
      301 tccgagagtt ctctaccaag aagtcacccg ctcggtttga tcaacggcac gatgaaattc
      361 gtttgactgt cggcgacgca ctcggatagt ttccaggacg tgccgtccga gcggtggaaa
      421 acttaaagaa attggaggat gaaaaatgat atcgcagatt cgcatttata cgatcaacaa
      481 gggtgagatg gataactttc tcaagcactt caaggatgac atcgtcgcct tgcaccaaaa
      541 aatcggtgtg ccgattgtcg gtagctgggt aaaccggccg caaaacgaat tcatctggat
      601 tcgcagctac aaagacaagg cggagatgga agcgaagggc aaagagtttc aagccgcggt
      661 tgccaccgcc ggagtgaaac tcggaagcaa cgtcgccaag atggaagtgc gcgaggtcga
      721 agcggcattt gcctaggttg attacggatc aagagaacga cgaggagccg ggataggacg
      781 gtagcgacgc agtagcctgt tactcgatag acggacggct tttcaaggct cttctaacta
      841 tggcagatat tgctcgatct cagaggagct cgacttgagt tgcccggata acgagctcct
      901 cttgccggaa tcgcttttcg agatcctttc tgtatccgga ccaccacttg cggttgagac
      961 gctcggtcat gacctcgaag ataaccacct cgtcacggct cacatcctcc tggccctcgc
     1021 gccatactcc ttccgcgggt gagcgtcgga aggcggtaat gccgccaaac cgttttgcca
     1081 gttcatcgcg cacgcggtca aattcgtccc gcgaaaacag tcgtttgtca ttgtcatgaa
     1141 gtggtaagag cagttggatc agattcataa gattttacct tttagatcga ttgctagtta
     1201 ttgtaacggc acggcagtca atggcaagcg acaataggtg agcggaggtc tccggactaa
     1261 caattcattc gtcccgctga atttccgtcg aagcagtgat tccagtcaat tagcgaatcg
     1321 gctatttgct ctagcggcga atacttacca tcgtcatttt ccaatttgac ttgtcgcgcg
     1381 tcgcgtcgca tggtcgttga gatgcgcaac gaaaatcgtg aggagataga ttgctatgcg
     1441 attcggactt gagtttggca gttacccttc ggagttgccg gcggggaagg tgtgtgagca
     1501 ggttgtcgag cgggcgcagg tggcgcagcg aaataatttc gaggcgctat tcgtcgccca
     1561 gcattttctc accgggccgg aggcggcgat cttgcagtcg ctaccgttgc tctcttatct
     1621 ggccggccgg gtgccgggca tgtacgtcgg cacttcgatc tttcttttgc cgttgcacca
     1681 tccggtaatg gtcgccgagt acatgtcgac gctcgacaat ttgtccggcg gcaaactgct
     1741 gttcggcgtc ggccagggat atcgcgaaag cgagttcaat tccttcggca tcgacctacg
     1801 cgagcgccgc ggccggttag tcgagagtct agagatcatc cgccgccttt ggaccggcga
     1861 tgccgtgacc tttcatggca agtatttcca tctcgacaac gtgatgatgg ggcccagacc
     1921 ggtgcaggtt cccggtccgc caatcttgct cggcgccgat tccgtcagga ccgtcgccaa
     1981 agtgccggag gtggcggacc actggatcgc cagccggcgc cactcgaagg gcttcttgcg
     2041 ggaagcgttg ccggtctaca aagccgcgct ggagaagcaa ggccggccgt acaaaggctt
     2101 gtttattttt cgcgatctgt gcatcgccga cagcaccaaa gaagccgaag agcgcatccg
     2161 cgagggctat gagcggcgtt acctgcgcta ccagcaatgg ggccagcctg gcgaacgcta
     2221 cgatctcaag ttcgacgagt tgaagcgcga tcgtctgatc ctcggctgtc ccgccgaggt
     2281 cgtcgagcag gtgatgtcct actacgagga gttcaacgcc gaggtcatgt ggttcatggt
     2341 cgactggccg ggcgtggacc cgcgcttcgc cttggaaacc atcgagcgtt ttggcgccga
     2401 ggtgattccc gcagtcaatc gccggacgcg gccttgtcca ctgccgtgat atagttccaa
     2461 aacttgagtc gagcaggtta ggttcgcggc cattcgtcag ggcttggttc aaatcgagct
     2521 gggcaggaaa aatccctctg aatctttctt tacaatggga gacttcgagc ggctctccgg
     2581 gattttatcg gctctgtttt cactccgcct acgcctgtga tacaaaaatt gtttcacgct
     2641 aagctagtgc cggcatttaa caggagtttt tcatgcaaca agaagtctta gaccaggtcg
     2701 atcgtgaccg tatcgtagag atggcgagta atctcgctaa catcgtgagc attaccggcg
     2761 acgaggaaaa cgtcgccaag tatctcggca gcgagttgga aactctcggg atgaaagtcg
     2821 agtatcaata tgtcgaagac ggccggccca acgtcatcgg cacgctcaaa ggcagcggcg
     2881 gcggcgcgac cttgatgttc aacgcgcata tggatcactt cgatgcgccg caggagaccg
     2941 tggtcgacga agaccgcatc tacggccgcg gcttggtcaa catgaaagcg gcgtttcctt
     3001 gctacatcga agcggtggcg gccctgcaaa aagccggcgc gaagctcaag ggcgatttga
     3061 ttatcgccgg cgtggtcggc gaaatcgaaa aggcgcaaat cggtcgtttt cacggcaaag
     3121 agtatcgcgg cgccggcgtc ggcgcgcgtt atctgatgga ccatggcgta atggcggata
     3181 tgtgcatcat cggcgagccc accggcttgc aattgcaaat cggcaactcc ggtttgctct
     3241 gggcgcgggt cagcgtcgac ggcaaggcga caaagttcgt gctcgccgcc tgcaaagtcg
     3301 cgcaagcgat tcaagactgg gagaaagaat accagcgcaa aaatccccac aagttcatgc
     3361 tgccgacggt acagatcggt gccatcgacg gcggcaatgc attcaagccc gggacgcggc
     3421 cgacgacgga catattcgtc atcgtcaaga tgctgccccg tgccgtgccg ttggcgatca
     3481 agcgcgatct cgaagcggtc tgtgaaagag tccgtaaacg cgagcccgat attctcgaca
     3541 ttcgcgtcga actctatctc acgactcccg gttatgaaat ctccaagagc gagccattgg
     3601 tgaaagttat ggagagcgcc catcgcacag tcttcaatgg accggtgccc tacgccaagc
     3661 cgatccgtta cggtatcacc agcgacggct cgcgcatcgc ccagtacggc gtgccggcga
     3721 tcacctacgg tcccggcttc ggcactcatt tggtcgatcc gaccgaaacc aaagcgggcg
     3781 aggagtggga ttcacccacc ggcgtgcgcc gcggcgtcgg catcaccaat atggtcaact
     3841 gcacgaaagt ttacgccatg gcggcgctgg atatttgcaa tcgcaagaaa gaggaagtgg
     3901 cgaaatcgta aaactgccgt tcaaggttca aaggttcaaa cgttcaacgt tcggcccggt
     3961 cttctctgac attgaacctt tgaaccttga accgttgaac aaaaaaatat atttgctctc
     4021 ggccaccttc gcaccgcgaa aagactcgta caaggcattt tcatggactt aaccttcgcc
     4081 cactaccgga accgcttctg catgttccgc acctactatg ccttggctat cggcaaggtg
     4141 aaggcggacg gcatcaacat gaaagtcatc gagctgcccg atccgccgag caaaga
//
LOCUS       DASQKS010000612         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2002943, whole genome shotgun sequence.
ACCESSION   DASQKS010000612 DASQKS010000000
VERSION     DASQKS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002943"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VE170_15705"
     CDS             complement(<1..194)
                     /locus_tag="VE170_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ16943.1"
                     /translation="MAAGIEQRRIKANGIDFNVAVSGANGSPSIFCLHGFPEGSISWR
                     PVMEALSEARFFAPDLRGYGP"
     gene            complement(215..1435)
                     /locus_tag="VE170_15710"
     CDS             complement(215..1435)
                     /locus_tag="VE170_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HYJ16944.1"
                     /translation="MNIDVNQLQPRRTASPALKLLHETLFALLKIERRFDPFFRPALD
                     AIFQEPLARGLQFLTNLWRDREILRLAEERKLPDEEAYLDSIISEMAAHMRQLYQPGS
                     YERGGNTKTHGVVRGEFTVRGDLAAPYRHGVFAEPRTFPAWVRFAGPGPAPPADIDDV
                     GVLSIGIKLMGVPGQKLLDDEKFTQDFTGITTPIFTTPDVKENAKLQARSRAGVPLFY
                     FISPGDSHLLDALMQGLWARTHTSPLEARYWSCVPYLLGAGAAMQYSIVPKAPRRSRV
                     PRLPSRPPDNYLRENMAATLSREDAEFDFTIQLQTDAHLMPIENASVRWPENLSPPKT
                     VATLRIAKQKFDSPAQLAFAHHLSYNPWHSVAEHRPLGNQNRGRKRIYQELSRLRQEM
                     NQTPHREPTGDEAF"
     gene            1706..>2879
                     /locus_tag="VE170_15715"
     CDS             1706..>2879
                     /locus_tag="VE170_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16945.1"
                     /translation="MNRRISLGLLLVTALLAAGCGRKSPLPGTVKDEALLAGRDAKSF
                     PAADEDYFKAMDGAAALTVDEVKGRNNWIVWSGGNDRFWDYLVSKSFRAVNLLKIISS
                     HPKTPHLTRDKRWEYLGVVNEPCFEKPKGPNADRFGLWLDIRSKDCPADPFENEQKYP
                     GVKIGARGKNLPVGSYYGYATGILGLRLFPNPAFDEAAAKKWDAERFYTDANYYNDKS
                     LVRPYRVGMSCGFCHVGPSPVRPPEDPENPKWENINSNPGSQYFWVDRVLFWEKDPAN
                     FVYQLIHTSLPGTLDTSFVSSDYINNPRTMNAVYNVGARLKIAERWGEEKLAGGGVNN
                     KQFQHYKRTAVLPQYYKEPDKVWTPHVLKDGADSVGILGALNRVYINIGLFSEEWLL"
BASE COUNT          601 a          788 c          862 g          628 t
ORIGIN      
        1 gggccgtagc cgcgcaaatc aggcgcaaag aagcgcgcct cgctcagtgc ttccatcacc
       61 ggccgccaac tgatcgagcc ttctggaaag ccgtgtaagc agaagatgct cggagatccg
      121 tttgcgcccg agaccgcgac attaaaatcg atgccgttgg ctttgatccg tctttgctcg
      181 ataccagctg ccatcgatgt catcactcct ctgcttagaa tgcctcatct ccagtaggct
      241 cgcggtgcgg tgtctggttc atttcctggc gcaggcggga gagttcttga tagattcttt
      301 tcctgccgcg attctggttg ccgagcgggc gatgttcggc gacgctgtgc cagggattgt
      361 aggagaggtg atgagcaaag gcgagctgcg ccggtgagtc gaatttctgt ttggcaatgc
      421 gcaaggtcgc caccgttttg ggcggggaga gattctccgg ccagcgaacc gacgcatttt
      481 cgatgggcat aaggtgcgcg tcggtttgca attgaatcgt gaagtcgaac tcggcatctt
      541 ctcggctcag ggtggcagcc atgttttcgc gcaggtaatt gtccggtggg cgcgacggca
      601 ggcgcggcac gcggctgcgc cgaggcgctt ttggcacgat cgagtattgc atcgccgcgc
      661 cggcgccgag caaataagga acgcagctcc aatagcgggc ttcgagcgga ctcgtatggg
      721 tgcgcgccca cagcccttgc atcaaggcat cgagcaaatg ggaatcgccg gggctgatga
      781 aatagaataa cggcacgccg gcccggctgc gcgcctggag cttggcgttt tccttgacgt
      841 cgggggtggt gaaaatcggt gtcgtaatcc cggtgaaatc ttgtgtgaac ttttcgtcgt
      901 ccaggagttt ctgccctggc acgcccatca gtttgatgcc gatgctgagc acgccgacgt
      961 catcgatatc ggcgggcggg gccggacccg ggccggcaaa gcgcacccag gcgggaaatg
     1021 tccgcggttc ggcgaagacg ccatggcggt agggtgcggc cagatcgccg cgcacggtga
     1081 attcgccgcg cacaacccca tgagttttag tattgccgcc gcgttcatag ctgccgggct
     1141 ggtaaagttg ccgcatgtgg gcggccatct cgctgataat cgaatccaag tatgcctctt
     1201 catcgggtaa tttgcgctcc tcggcgagtc gcaaaatttc ccggtcgcgc cataagttgg
     1261 tgaggaattg cagcccgcgc gccagcggct cttgaaaaat tgcgtcaagt gccgggcgga
     1321 aaaacggatc gaagcgccgc tcgatcttga gcagtgcgaa gagagtttcg tgcaatagct
     1381 ttagcgccgg cgaagccgtg cgccggggct gtagttggtt aacgtcgatg ttcaccaatc
     1441 cctcgcgagc aaccgtccgc acccgatgac gagttcaaag actgcgccaa ttttcgcaat
     1501 atacgccact aatgaagttc gaggcaactg ttttggccgc gtaggcgtcg gcatgcggcg
     1561 tagcctcaag tcaggcgatg gttctgacaa attttcttgc catatcgctg tgacttgcgg
     1621 tactttttgc ctgcgagtgg ccggacaaat tgttaaatcc caccgcgcta ccttgataat
     1681 gacatctcgc tgaaagggag tgtacatgaa ccgccgtata tctttgggcc tgttacttgt
     1741 gaccgctctg ctagctgccg gttgcggcag gaaatcgccg ctgcctggta ccgtcaaaga
     1801 cgaagccctc ttggccgggc gcgacgcgaa aagttttccg gccgctgatg aagactattt
     1861 caaagccatg gatggcgccg ccgcgctcac cgtcgacgaa gtcaaggggc gcaataactg
     1921 gattgtctgg agcggcggca atgaccgatt ctgggattat ctggtgagca aaagttttcg
     1981 cgccgtcaac ctgttgaaaa taatttcgtc acatcccaag acgccgcatc tgacccgtga
     2041 caaacgctgg gagtatctag gtgtagtgaa cgaaccttgc ttcgaaaagc ccaaggggcc
     2101 caacgccgac cgttttggcc tgtggctcga catccgaagc aaggattgtc ccgccgaccc
     2161 ttttgaaaat gagcaaaagt atcccggcgt gaaaattggc gcgcgcggca agaatctgcc
     2221 tgtcggttcg tattacggct atgccaccgg aatcctcggt ctgcgcttat ttcccaatcc
     2281 ggctttcgac gaagcggcgg caaagaaatg ggatgcggaa agattttaca ccgacgcgaa
     2341 ttattacaac gataagagtc tggtgcgccc ctaccgggtc ggcatgtcct gcggcttttg
     2401 ccatgtcggg ccgagcccgg taagaccgcc ggaggatccg gagaatccca aatgggaaaa
     2461 catcaactcc aatcccggct cgcaatattt ttgggtcgac cgcgtgctgt tctgggaaaa
     2521 agatccggcg aatttcgtct accagttgat tcacacctcg ttgccgggaa ccctcgatac
     2581 ttcgttcgtg tcgtccgatt acatcaacaa tccgcgcacg atgaacgcgg tctataacgt
     2641 cggcgcccgg ctcaagatcg ccgagcgttg gggtgaggaa aaactcgccg gcggcggtgt
     2701 caacaataaa cagttccaac actataagcg gaccgccgtg ctgccgcagt attacaaaga
     2761 gccggacaaa gtttggaccc cgcatgtttt gaaggacggc gccgactcgg tcggcatcct
     2821 tggcgccttg aatcgggtct atatcaacat cggattgttc agtgaggaat ggctgcttc
//
LOCUS       DASQKS010000613         7644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5796566, whole genome shotgun sequence.
ACCESSION   DASQKS010000613 DASQKS010000000
VERSION     DASQKS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5796566"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..293
                     /locus_tag="VE170_15720"
     CDS             3..293
                     /locus_tag="VE170_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HYJ16946.1"
                     /translation="MTFAWYAHLKNLSDRPWYVAALVSWGIASFEYLIQVPANRLGHT
                     QMTVPQLKIMQEVITLSVFAPFAVLYMNEPLKWDYLWAGACLLGAVYFMFRS"
     gene            complement(422..1363)
                     /gene="trxB"
                     /locus_tag="VE170_15725"
     CDS             complement(422..1363)
                     /gene="trxB"
                     /locus_tag="VE170_15725"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HYJ16947.1"
                     /translation="MDKQSHYRVVILGSGAAGLTAAIYSARANLKPLVIEGSQPGGQL
                     TITTDVENFPGFPKGIMGPELMEEFKKQAVRFGTETFFAEVTAVDLKQRPFKVTASKE
                     FYTCDTLIVATGATAKLLGLESEMKLMGHGVSACATCDGFFFKDKELVVVGGGDTAME
                     EATYLTKFASKVTVVHRRDKLRASKIMQERAEKNGKISFVWDTAVEEVYGDPKAGGVT
                     GVKFKNLKTGKNSDFKCDGLFIAIGHEPNSKLFAGQLDLDPTGYILTHDGSKTNVSGV
                     FACGDVQDHVYRQAITAAGSGCMAAIDAERFLENQEG"
     gene            1411..1890
                     /gene="moaC"
                     /locus_tag="VE170_15730"
     CDS             1411..1890
                     /gene="moaC"
                     /locus_tag="VE170_15730"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYJ16948.1"
                     /translation="MAQLSHLDEQGRARMVDVSEKEVTSRIATACGTIHMLPETLALI
                     LDDKIEKGDVFSVARVAGIMAAKKPSELIPMCHPLNITSVAIDLTPGHNPARVDIEAS
                     VRVSGKTGVEMEAMTAVSVAGLTIYDMCKAVDREMTIGEIRLVKKSGGKSGTFIRTE"
     gene            1974..3149
                     /locus_tag="VE170_15735"
     CDS             1974..3149
                     /locus_tag="VE170_15735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HYJ16949.1"
                     /translation="MQQIFLKPNRDAPIRRGHPWIFSGAIERCEGDDQIADVADIFDS
                     KKTWIGRGLYNPKSALRVRLLTWKKETIDQVFFAARLDQALAFRAEQLGAANDAYRLV
                     NGEGDLLPGLIVDRYANYFVCQFFTAGMDSFKEEIVAALSELCPATGIFERSEGRVRE
                     EEGLEAAVGVLAGEPPPETLAIRENGHQFLVDLRRGQKTGFFLDQRDSRQFLGTLTRS
                     RSVLNCFAYTGGFSVYAYAGGAREVVTFDSSMPALELAEKNLALNGYGGAPGEVIKGD
                     AFAYLKETDSKFDVIVLDPPSLAHRRGDLDAATGGYKFLNLHALKHLNPGGILLTFSC
                     SQHLPRELFQKVIFGAAIDAGRKISIVKQLGHAIDHPVSLHHPEGEYLKGLALRVMD"
     gene            3386..4078
                     /gene="radC"
                     /locus_tag="VE170_15740"
     CDS             3386..4078
                     /gene="radC"
                     /locus_tag="VE170_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYJ16950.1"
                     /translation="MTLSYTIKEWPEADRPREKLLARGASALSETELLAIILRNGDAA
                     SGNSAIDHARLLLSQFGGLKGIDEAAVAELCSIKGIGPAKVAQLKACMEIGKRNGSLK
                     WETGQLLNSAGDVFKHFRESLGRDKRESFYVVLLDNKNRKMREVKISEGSLTASLVHP
                     REVYNPVIRESAAGVIFVHNHPSGDPAPSPEDIDITKRLKDVGDVMGVRVLDHVVIGH
                     DRYFSFNEKGML"
     gene            4289..5719
                     /locus_tag="VE170_15745"
     CDS             4289..5719
                     /locus_tag="VE170_15745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYJ16951.1"
                     /translation="MSALQLGRPKRSFAPGPGVIYTFLIAIVIVGGYFLVPRFEWYKP
                     QIKITPDSETLGLGTLQIEVTEQGTGLKSFSAVLNSGGTDYPLASEQYTEPTLQKKLT
                     VVLSSKLAGLKEGPAVLRVSARDNSLWNLFRGNETAIQKNLTIDITPPTLEIVADDRY
                     VNFGGVGLIVYKPSADTVTSGIKVGNYFFPGYKGQIASQPEHYVAFFAHAYNVGEDER
                     AALVATDKAGNMREMRLAYTLKNVKYKKSTLQISEDFLRNKVTPLLNDVGARQGSPKE
                     IFIKVNKGLRKENEDKIKAITEKSSPSKLWDGAFSQLSNSKVEANFADARTYIYNTEE
                     IDKAYHLGYDLSVTKHYPAEAANHGIVAFVGDLGIYGNTVIIDHGLGLFTLYSHLSSI
                     DVKVGDKVTQRQPIGKTGETGLAAGDHLHFGVYLHGVAILPVEWWDQKWIKDNVQPKL
                     DGQTGESTAEAQEAKTTAKTGRKRRR"
     gene            complement(5773..6972)
                     /locus_tag="VE170_15750"
     CDS             complement(5773..6972)
                     /locus_tag="VE170_15750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16952.1"
                     /translation="MSKAENIEAISLPAANWGRLAANCTAHMTEHLFNAVVSVILPLI
                     TASLGLSLSQAGALASIRTLMAGVASFPSGFLADLARRRNLLLGGCIALIGLASLGLS
                     ASSSFLLLMVFMGLGGFGGGWFHPQSLAILSEKYRTQKAFALGVHDSSANLGEVIGPL
                     AMGLMLNFFDWRTVLLFWAVPGLLVGAIYALAGSEGLVAVPRGRDYRKAIWNDVLKNK
                     TVCGLVAVSTLRAMGQTALASFLPLYLALHLQLPAAVTGAYMSILFLCAGVAPALVGW
                     VSDRIGHRYLIVLFSLLSVATVAAIPYLGSGAVLVAGLGALGALLWALRPVIVSAAMS
                     EAPQQLSGSIVAFIYGANMGLSFLAPLLTGIVADQYGLPTAITAIAIFPLLASLICFV
                     LLIQRHR"
     gene            complement(7348..>7644)
                     /locus_tag="VE170_15755"
     CDS             complement(7348..>7644)
                     /locus_tag="VE170_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ16953.1"
                     /translation="VDMQGKICGVISDRDILRAMAMSRDSRSKSLNQIMTRNPITVKR
                     ETSILDAVSKIISKKINCLPVVEDDDGTVCGIVTSTDLLKSYQQVLEMVHKQAG"
BASE COUNT         1789 a         2256 c         2075 g         1524 t
ORIGIN      
        1 tcatgacctt cgcctggtat gcgcacttga agaacctaag cgaccgcccc tggtacgtcg
       61 ccgcactggt aagctggggc atcgccagtt tcgagtatct gattcaagtc ccggccaacc
      121 gcctcggcca cactcaaatg acggtcccgc agctcaaaat tatgcaggaa gtgatcacgc
      181 tctcggtctt tgcgccgttc gccgtgcttt acatgaacga gccgctcaaa tgggattacc
      241 tttgggcagg agcgtgctta ctgggagctg tgtatttcat gtttcgctcg tgacccgtcc
      301 ctgccatagc gaggcctaat tgaataacgc gacactcaat gcagttcacc ggtcaaaaaa
      361 atcgctctcg caggtcatac agtctcggca ttcgcgatga acgcttgcgg atgcaagcta
      421 ctcatccttc ctgattctcc aaaaatcgct ccgcatcgat cgccgccatg cagccgctgc
      481 cagcggcggt gatcgcttgg cggtagacgt gatcttggac gtcgccgcag gcgaagacgc
      541 cggagacgtt ggtcttggag ccatcgtgag taagtatgta gccggtcgga tcgagatcca
      601 actgaccagc gaacagtttt gaattgggct cgtggccgat ggcgatgaac aggccatcgc
      661 atttgaaatc ggaattcttg ccggttttta aattcttgaa tttaactccg gtgacgccgc
      721 cggctttagg atcgccgtaa acttcttcca ccgcggtgtc ccaaacaaaa gagattttcc
      781 cattcttttc ggcgcgctct tgcatgatct tggacgcgcg cagtttgtcg cgccggtgca
      841 cgaccgttac tttgctggcg aatttggtga ggtaagtcgc ctcttccatc gccgtgtcgc
      901 cgccgccgac gacgacaagt tctttgtctt tgaagaaaaa accatcgcag gtggcgcacg
      961 ccgacacgcc atggcccatg agtttcattt ccgattccaa gcccagcaac ttcgccgtgg
     1021 cgccggtagc gacaatcaac gtgtcgcaag tgtagaactc tttgctggcg gtgactttga
     1081 atggccgctg tttgaggtcg accgccgtca cttcggcgaa aaacgtctcc gtgccgaagc
     1141 gcacagcctg tttcttaaac tcctccatca attcgggacc catgatgcct ttggggaaac
     1201 cggggaaatt ttcgacatcg gtggtgatcg tcagttgccc gcccggctgc gacccctcga
     1261 tgaccagcgg tttaagattg gcacgggcgc tgtagatcgc cgcggtgaga ccggcggcgc
     1321 cggaaccaag aatgactaca cggtagtgac tttgcttatc catccggttt ctgtaacata
     1381 atttttccag gggagaaaga aaccaaacct atggcacaac tcagtcactt ggacgaacaa
     1441 gggcgcgcgc gcatggtcga cgtgagcgaa aaagaagtca ccagccggat tgcgacagcc
     1501 tgcggcacca ttcacatgct gccggaaact ttggcgctga tcctcgacga caagatcgaa
     1561 aagggcgatg tcttttcggt ggcccgcgtc gccggcatca tggcggcgaa aaaaccttcc
     1621 gaactgattc ccatgtgcca tcctctcaac atcacctccg tcgcaatcga tctcacaccg
     1681 gggcacaatc cagcccgggt cgacatcgaa gcttcggtgc gggtcagcgg caaaaccggc
     1741 gtcgaaatgg aagccatgac cgcggtgtcg gtggccggcc tcaccatcta cgacatgtgc
     1801 aaagccgtcg accgcgaaat gaccatcggc gaaatccgcc tggtcaaaaa gagcggcggc
     1861 aaaagcggca cgttcatcag aacggagtag aggtatttcc gggcgttccg gaacccagca
     1921 ctccagtact ccgaattcat accttgctcc acacacggaa atttgaatgc cccatgcagc
     1981 aaatatttct caaacctaac cgtgacgcgc cgattcgccg gggccatccg tggatatttt
     2041 ccggcgccat cgagcgctgc gaaggcgacg atcagattgc cgacgttgcc gatatcttcg
     2101 acagcaagaa aacctggatc ggccgcggcc tctacaatcc caagtcagcg ctgcgcgtgc
     2161 gcctgctgac ctggaagaaa gagaccatcg atcaagtttt tttcgccgcc cgcctggacc
     2221 aagcattggc ctttcgcgcc gagcagttgg gcgcggccaa tgacgcctac cggctggtca
     2281 atggcgaagg cgatcttctc cccggcctga tcgtcgaccg ctacgccaac tatttcgtct
     2341 gtcaattttt taccgctggc atggattcgt tcaaagaaga aatcgtcgcc gcgttgtcgg
     2401 agctttgccc cgccaccggc atattcgagc gcagtgaagg gcgggtgcgt gaagaagaag
     2461 ggctcgaagc tgcggttggc gtgctcgccg gcgagccgcc gccggagacc ctcgccattc
     2521 gggagaatgg ccatcagttc ttggtcgatc tacggcgcgg gcaaaaaacc ggtttctttc
     2581 tcgatcaacg cgacagccgg caatttcttg ggacgctgac gcgcagccgg tcagttctca
     2641 attgcttcgc gtacaccggc ggcttttcgg tttacgccta tgccggcggc gccagggaag
     2701 tggttacctt cgattcgtcc atgccggcgt tggagctagc cgaaaaaaat ttggcgctga
     2761 acggctacgg cggcgcccca ggcgaggtca tcaagggcga cgccttcgct tatttaaagg
     2821 agaccgatag caagttcgac gtgatcgtgc tcgacccgcc atcgctggcg caccggcgcg
     2881 gtgacctgga cgccgccacc ggcggttata aatttctaaa tctgcatgcg ctaaagcatc
     2941 tcaatccggg cggcatacta ctcacctttt cctgctcgca gcatttgccg cgcgagctgt
     3001 ttcagaaagt catttttggc gccgccatcg acgccggcag aaaaatctcg atcgtcaaac
     3061 aactcggtca tgccatcgac cacccggtaa gcttgcatca ccccgaaggc gaatacctca
     3121 agggattggc actccgggtg atggattgac ccgaatctac ggtttcgctg tcaccccgaa
     3181 caagggccac gggtctcggc gtgagaactt tggcaatatt ggcgaatggc gaaacttccc
     3241 ggccgtgccc tcgaacgact gaggtggaga accgtgacgg gttgcagccg tcgacacacc
     3301 ctttgagccc ccgcgctgac tgtgatagac gaagctcgca accgcggacc ctcaaggcgc
     3361 gttgacgctg tgccgcgagg tgttcatgac attatcctac accatcaaag agtggcccga
     3421 ggccgaccgg ccgcgagaaa aactgctggc cagaggtgcg tcggctttga gcgaaaccga
     3481 acttttggca attatcttgc gcaatggtga cgccgcgagc ggcaatagcg ccatcgatca
     3541 tgcgcggctc ttgctcagcc aatttggcgg cctcaaaggc atcgacgaag cggccgtggc
     3601 cgagctatgc agcatcaagg gcatcggccc cgccaaggtc gcccagctca aggcttgcat
     3661 ggagatcggt aaacgcaacg gtagcctgaa atgggaaact ggccagctgc tcaattccgc
     3721 cggcgatgtc tttaaacatt tccgcgaaag cctcggccgg gacaagcgcg aatctttcta
     3781 cgtcgtcctg ctcgacaaca aaaaccgcaa gatgcgcgag gtcaagatct ccgagggatc
     3841 gcttaccgcg tctctggtcc atccgcgcga agtctacaat ccggtcattc gggaatccgc
     3901 cgccggcgtc attttcgtgc acaaccatcc gagcggcgac ccggcgccga gccctgagga
     3961 tatcgatatc accaaacggt tgaaagacgt gggcgacgtc atgggcgtgc gcgtgctcga
     4021 ccatgtcgtg atcggccatg accgttattt tagcttcaac gaaaaaggca tgctctgatt
     4081 cgcgctgccg aatccagcaa tttttttcaa cacctcatcg tagccgccag ccactctgct
     4141 attttgaccg cggaggaaat ctcttccggt tttttcggca tcactgcgaa attgagcagt
     4201 gataccggta gttttgattg acatcattac cgacggcgtt tatcttgttg gactatctca
     4261 cagcttctct aaattcgtta cataaagcat gagcgcactc caactaggca gacccaagcg
     4321 aagctttgcc cccgggccag gcgtcattta cacctttctt atcgccatcg taattgtcgg
     4381 tggttacttt ctcgtcccgc gcttcgaatg gtacaaaccg cagattaaga ttacccccga
     4441 tagtgaaacc ctgggcctgg gaacgttaca gatcgaggtc accgagcaag gcaccgggtt
     4501 aaaatcattt tcagcggtgc tcaattccgg cggcaccgat tatccactcg catccgagca
     4561 gtacactgaa ccgacactgc aaaaaaagct caccgtcgtg ctatcgtcga aactcgccgg
     4621 cttgaaggaa ggtccggcgg tactgcgggt cagtgcccgc gataattcgc tgtggaacct
     4681 gtttcgcggc aatgagacgg cgattcagaa aaacttgacc atcgatatta cgccgccgac
     4741 gctggagatc gtcgccgacg accgctatgt caactttggc ggtgtcggcc tgatcgttta
     4801 caagccttcc gcagacacgg tcaccagcgg gataaaggtc ggcaactatt tttttcccgg
     4861 ctataaaggc cagatcgcga gccaaccgga acattacgtc gcctttttcg cccatgctta
     4921 caacgttggc gaggatgaac gagcggcgct ggtcgccacc gataaagccg gcaacatgcg
     4981 agaaatgcgg cttgcctata ccctcaagaa cgtcaaatac aaaaagagta ccctgcagat
     5041 ttccgaagat tttctccgca acaaagtcac accgctgttg aacgacgtcg gcgcgcgtca
     5101 aggcagcccc aaagagattt tcatcaaagt aaacaagggg ctgcgcaaag aaaacgaaga
     5161 caagatcaaa gcgatcaccg aaaaatcgtc accgtcaaag ttgtgggatg gcgctttcag
     5221 ccagctcagc aactccaagg tggaggccaa cttcgccgac gcgcgcacct atatttacaa
     5281 taccgaagaa atcgataagg cctaccattt aggctacgac ctgtcggtga ccaaacacta
     5341 ccctgccgag gcagcgaatc atggcatcgt tgccttcgtc ggtgatctgg gaatctacgg
     5401 caacacggtc atcatcgatc acggcctcgg cttgttcacg ctctattcgc atctgagctc
     5461 aatcgacgtc aaagtcggcg acaaagtcac gcaacgacag cccataggca agaccggcga
     5521 aaccggccta gccgccggcg atcacctgca tttcggcgtc tatctgcacg gcgtcgcgat
     5581 cctgccggtt gaatggtggg atcaaaaatg gatcaaagac aacgtccagc ccaagctcga
     5641 cggccagacc ggtgagagta cagcggaagc gcaggaagcc aaaacaaccg caaaaaccgg
     5701 gcgcaaacgg cgccggtaat ttgaacccag cttgacccgc gcgacaatac ctcgcctcaa
     5761 aaaagcctgg attcaacggt gccgttgaat cagcagaacg aaacaaatca aagaagctaa
     5821 cagggggaat atcgcgatgg cggtgatcgc cgtgggcagg ccgtattggt cggcgacgat
     5881 gccggtcaaa agcggcgcca aaaaagacag acccatattg gcgccataga tgaacgccac
     5941 gatgctgccc gacaactgtt gcggcgcctc cgacatcgcc gccgacacaa tcaccggccg
     6001 cagcgcccag agcagcgcgc ctaaagctcc caaaccggca accagcacgg cccccgaacc
     6061 caagtacggg atcgcggcca ccgtagccac actcaagagc gaaaacagca cgatcaaata
     6121 acgatggccg atgcggtcgg aaacccaacc gacgagagcc ggcgccaccc cggcgcagag
     6181 aaataaaatc gacatgtaag cgccggtgac ggcggcgggc aactgcaagt gcaacgccag
     6241 atagagcggc agaaaagacg ccaaagccgt ctgtcccatg gcgcgcaaag tcgacacggc
     6301 aacgagcccg cagacggttt tgttcttgag cacatcattc cagatcgcct tgcggtagtc
     6361 gcggccccgc ggcacagcca ccaagccttc gctgccagcc agcgcgtaga tcgcgccgac
     6421 cagtaacccg ggaaccgccc agaacaacag cacggtgcgc cagtcgaaaa aattgagcat
     6481 caaacccatc gccaacgggc cgatgacttc accgagattg gcgctactgt cgtgcacgcc
     6541 aagagcaaag gccttctgcg tgcggtactt ctcggacaaa attgccaacg actgcggatg
     6601 aaaccaaccg ccgccaaatc cgccgagtcc catgaaaacc atcagcagca agaaactgga
     6661 ggacgcgctc aagcctaacg aagccaagcc gatcaaggcg atgcagccgc cgagcaacaa
     6721 attgcgccgg cgcgccaggt cggcgagaaa accggaaggg aaactagcca cgcccgccat
     6781 caacgtgcga atcgacgcga gggcgccggc ctgtgacagg ctcagtccca acgacgcggt
     6841 gatcagcggt aaaattacac tgaccacggc attgaacaga tgctcggtca tatgcgcggt
     6901 gcagttggcg gcaagccggc cccaattggc ggccggcaaa cttatagctt cgatattttc
     6961 ggcttttgac aaactatcgt ccttggaggt tttaatattt tcatatgctg attttaccgg
     7021 cgtggcaagc tcggtggttg acccggaatg gggatccggc aaacccggat caatcgatct
     7081 ctacctgctt ttttcgcgac gagtggccgc ggacgattct tagtgaggat ttgggaacgg
     7141 cgaagaattc cgccaaagct tcgatcaccg cgcgattggc cgcgccgtcc cgtgccggag
     7201 cacggactgc gacccgaaat tctgcttctg agattctctc gacaccgcta tggcgagcat
     7261 ttggcttaac cgcgaccgag aatttccgcg ccatgactga gtgtagcgcc cgttagaaat
     7321 cgatcaatgc ggacgtgaag gactcgatca gccggcttgt ttatgtacca tctcgagcac
     7381 ctgctggtag gacttgagca agtcggtcga cgtcacgatg ccgcagaccg tgccgtcgtc
     7441 atcttcgacc accgggaggc agtttatttt cttcgaaata atttttgaaa cggcgtcgag
     7501 gatcgaggtt tcgcgcttca ccgtgatcgg gttacgcgtc atgatttgat tgagggactt
     7561 tgaccgtgaa tcccggctca tcgccatcgc tcgcaaaata tctcgatcgg agatgacacc
     7621 gcaaatctta ccctgcatgt cgac
//
LOCUS       DASQKS010000614         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5390892, whole genome shotgun sequence.
ACCESSION   DASQKS010000614 DASQKS010000000
VERSION     DASQKS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5390892"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VE170_15760"
     CDS             complement(<1..576)
                     /locus_tag="VE170_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ16954.1"
                     /translation="MANTTEELLLDVRVAEASPLFKFYLNQEKKILGTIAVSIFLILW
                     ELVGNTFQLINPMFMSAPSLIWKAAVEMFSSGEIYNDLYISGIELAGGYLLSAVIAVP
                     FGIAIGWYKKVAYIFDPFVNAMNATPRVALLPLVIIWLGIGILSKVGIIFLGAVFPIL
                     INARDGVKTTPSNLLTAARSFGASEWMLFKTV"
     gene            complement(569..1069)
                     /locus_tag="VE170_15765"
     CDS             complement(569..1069)
                     /locus_tag="VE170_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYJ16955.1"
                     /translation="MPNNSSLPDFKAMAELRYQIRCFLRFSETAARHAGIEPQQHQLL
                     LAVKGLPKNLKPTIGVLAERMQLQHHSTVELVDRLADRGFLLRLRASDDRRQVLVKLT
                     RDGEEFLKKLSLHHLQELQSSGPKFVKILQGLIEGIEEPPDIAVNITPTESTKPTKQR
                     KEKNHG"
     gene            1324..1638
                     /gene="rplU"
                     /locus_tag="VE170_15770"
     CDS             1324..1638
                     /gene="rplU"
                     /locus_tag="VE170_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYJ16956.1"
                     /translation="MKYAVIRTGGKQYRVSEGDVVKIEKLDAAVGETITLADVLFVGN
                     DNDVKIGTPLVANATVTGEIVDQSRAKKIIVFKKKRRKGYSRQQGHRQYQTALKITSI
                     EA"
     gene            1651..1908
                     /gene="rpmA"
                     /locus_tag="VE170_15775"
     CDS             1651..1908
                     /gene="rpmA"
                     /locus_tag="VE170_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYJ16957.1"
                     /translation="MAHKKAGGSSRNGRDSNGQRRGVKVYGGQTVRAGNILVRQLGTK
                     IHPGSNVGMGRDYTLFSLVDGVVKYGRYDKTRKKVEVVPAQ"
     gene            2097..3137
                     /gene="obgE"
                     /locus_tag="VE170_15780"
     CDS             2097..3137
                     /gene="obgE"
                     /locus_tag="VE170_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYJ16958.1"
                     /translation="MKFIDETKIRAIAGDGGPGSVSFYRAKFVRRGPADGGNGGHGGD
                     VVAIADPQLSTLLDLRYRKEWKARPGKRGGDADCDGGRGDDCIIKVPVGTLIRDVVSG
                     ELLADLKSPGERAVIAAGGRGGKGNAHFASSTNRSPRFCQPGEPGVERDLEVELRLLA
                     DVGIIGLPNAGKSTLISVISAVRPKIADYPFTTLVPNLGVVSYGEGKSFVAADIPGLI
                     EGAHQGHGLGDKFLRHVLRTRVLVHMLDASQLDEKDPLAEWKAINREMELFDPELIDK
                     PQLVVANKIDLPEGRDLAKLLGRKLPKAYRPLYVISAATTEGVQALVYAIGRKLDEVK
                     QRSETDSDAAGA"
     gene            3118..>3773
                     /gene="proB"
                     /locus_tag="VE170_15785"
     CDS             3118..>3773
                     /gene="proB"
                     /locus_tag="VE170_15785"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYJ16959.1"
                     /translation="MTQLEHKKPILIRARRVVVKIGSQILSSKDGIEETRLRGLVRQL
                     AELQGGKRQVVVVSSGAVAAGMTQLGWKERPKTIPEKQALAAVGQIKLMALYERTFAK
                     FDISVAQVLLTAADLANRQRYINAKHTFQMLLGSSIIPIVNENDTVAVEEMKFGDNDH
                     LSALVATLLEADLLVILSDVEGVYDKDPRVHDEAQLIPLIAEARGLRQAIGGASVSAT
                     G"
BASE COUNT          871 a          975 c         1120 g          807 t
ORIGIN      
        1 cacggtcttg aagagcatcc actcggaagc gccgaaactc cgggcggcgg taagtagatt
       61 cgacggtgtg gtcttcacgc catcgcgcgc attgatcaag atcgggaaaa ccgcgccgag
      121 gaagataata cctactttcg agagtatgcc gatccctaac caaatgatta ccaggggtaa
      181 aagtgccacg cgcggggtcg cgttcatggc gttaacaaag ggatcgaata tgtaagctac
      241 tttcttgtac cagccgatgg cgatgccaaa gggaacggca atgacggccg ataacaaata
      301 accaccggca agttcgatac cgctgatgta aaggtcatta tagatttcgc cggaagaaaa
      361 catctcaacg gccgccttcc aaatcaacga cggcgcgctc atgaacatcg gattgatcaa
      421 ctgaaaggta tttccaacca gctcccatag aatcaaaaat atgctaaccg caatggtgcc
      481 gagaattttc ttttcctggt tcaggtagaa tttgaacaaa ggcgacgcct cagcgacgcg
      541 cacatccaaa agtagttctt cggtggtatt agccatggtt tttttccttt cgttgtttag
      601 tcggctttgt tgattctgtc ggcgttatat taactgcaat gtcaggcggc tcttcgatac
      661 cttcgatcaa accctgtaat atcttcacga atttcggacc ggaggactgc aattcttgca
      721 aatgatggag tgacagtttt ttgagaaact cttcgccgtc gcgggttagt ttgaccagaa
      781 cttgccggcg atcgtcgctg gcgcgcaagc gcaaaagaaa ccctcggtcg gcgagccggt
      841 cgacgagctc gacggtactg tggtgctgca actgcatccg ttcagcgagc acgccgatgg
      901 taggtttcaa gttcttgggt aatcctttca cggccaaaag aagctggtgc tgttggggct
      961 ctatgcctgc gtgccgagcc gcggtttcac taaagcgcaa gaaacagcga atttgatagc
     1021 gcaactcggc catggctttg aagtcgggaa ggctggagtt gtttggcata aatacatcgt
     1081 gttacgataa tatcgcgaca agatataaaa gtttcggcgg gtcttgtcaa ccgcggcggc
     1141 cagatttcgc caatcgcatt tgaccgtcat aaatgccctg taataggttc tgagcaagcg
     1201 accacacttg aggctgagaa cctggcttgg caaagacgtt caggtatctt agcgccgcgt
     1261 tcctggacaa aacgttgact ttggatcggt tttcctttaa aaactaactt tcggtgaaat
     1321 cctatgaaat atgcagtaat tagaaccggc ggcaagcaat accgcgtctc tgaaggagat
     1381 gtggtcaaga tcgaaaagct cgatgctgca gtgggcgaga cgataactct ggcggatgtc
     1441 ttgtttgtcg gcaacgacaa tgacgtaaaa atcggcactc ccctggtagc caacgccact
     1501 gtcacgggcg aaattgtcga tcagagccgc gccaagaaga tcatcgtttt caagaagaaa
     1561 cggcgcaaag gctacagccg gcaacagggc catcggcagt atcaaacggc gcttaaaatc
     1621 acatccatcg aagcatagcg cggagcggtt atggcacata aaaaagcagg cggcagctcg
     1681 cgtaacgggc gcgacagcaa cgggcagcgg cggggagtca aagtctacgg cggccagacg
     1741 gtacgcgcgg gcaatattct agtacgccag ctgggcacca agatacatcc cggctccaac
     1801 gtcggcatgg gtcgcgacta tacgctcttt tcccttgtcg atggcgtggt caaatatggc
     1861 cgttacgata agacgcgcaa gaaagtcgaa gttgtcccgg cgcagtaatt tctcattcga
     1921 ctcctatgaa agaagccccc tttcggggct ttttttttgt ctggaattcg gcggagtggg
     1981 gagaagctgc aagatattcg ttccgaacgg tccgggctaa gctggtggat cgggtttatt
     2041 tcggtcatgc ttcgatcatc gagctgctga accatggcta ttgaagaccg ctactcatga
     2101 aatttatcga cgagacgaag atccgcgcga tcgccggtga cggcggaccc gggtctgtga
     2161 gtttttaccg ggctaagttt gttcgccgcg gcccggccga cggcggtaac ggcggtcatg
     2221 gcggcgatgt ggtcgccatt gccgatccgc agctgtcgac gctgctcgac ttacgctatc
     2281 gaaaagaatg gaaagcgagg cccggtaagc gcggcggcga cgctgactgc gacggcgggc
     2341 gcggcgacga ctgcattatc aaagttccgg tgggtacgtt gatccgcgat gttgtcagcg
     2401 gcgaattgct ggccgaccta aagtcgcctg gcgagcgggc ggtgatcgcc gccggcgggc
     2461 gcggcggtaa gggcaacgcg cacttcgcat catcgaccaa tcgcagcccg cgtttctgcc
     2521 aaccgggcga gccgggtgtc gagcgcgatt tggaggtcga gcttcgcttg ctcgccgacg
     2581 tcggcattat cggcttgccc aatgccggca agtcgacctt gatctcggtg atctcagcgg
     2641 tgcggccgaa aatcgccgac tatccgttta cgacactggt gcccaactta ggcgtggtca
     2701 gttacggcga aggtaagagt ttcgtcgccg ccgacattcc cggcctaatc gagggcgcgc
     2761 accagggcca cggcttgggc gataagtttc ttcgtcatgt tttgcgtacg cgggtgctgg
     2821 tgcatatgct cgacgcctcg cagctagacg agaaagatcc tttagccgaa tggaaggcga
     2881 tcaatcgcga gatggagctt ttcgatccgg aattgatcga taagccgcaa ctcgtggtcg
     2941 ccaacaaaat cgatttgccc gagggccggg acttggccaa gctgctcggc aggaaactac
     3001 ccaaggccta tcggccttta tatgtgatct cggcggcgac caccgagggt gtgcaggcgc
     3061 tggtttatgc catcggccgc aaactcgacg aagtgaagca aagaagcgag accgacagtg
     3121 acgcagctgg agcataagaa gccgatcttg atccgggcac gccgtgtcgt cgtgaagatc
     3181 ggcagtcaga tcctctcttc caaggacggc atcgaggaga cgcgcctgcg cggcctggtg
     3241 cgccagctcg ccgaattgca gggcggcaag cgccaagtcg tggtcgtcag ttcgggcgcg
     3301 gtggcggcgg ggatgacaca actggggtgg aaagagcggc cgaaaacgat tcccgagaag
     3361 caggcgctcg ccgcggtggg acaaattaaa ttgatggcgc tttatgagcg gacatttgcc
     3421 aagttcgaca tcagcgtcgc ccaggtgttg ctaaccgcgg cagatctcgc caatcgccaa
     3481 cgttatatta acgccaagca tacttttcag atgctacttg gctcgtcgat cattccgatc
     3541 gtcaacgaga atgacaccgt ggcggtcgag gagatgaaat tcggtgacaa cgatcatctg
     3601 tcggccctgg tagcgacgct tctcgaggcc gacttgctgg tgatcttgag cgatgtcgag
     3661 ggcgtttacg ataaggatcc gcgggtccat gacgaggctc aactgattcc gctaattgcc
     3721 gaagcgcgcg ggctgcgcca ggcgatcggc ggcgcgagtg tgagcgcgac cgg
//
LOCUS       DASQKS010000615         6021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5393862, whole genome shotgun sequence.
ACCESSION   DASQKS010000615 DASQKS010000000
VERSION     DASQKS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5393862"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VE170_15790"
     CDS             <1..1134
                     /locus_tag="VE170_15790"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ16960.1"
                     /translation="PYCTMFLGDLGAEVVKVEQPGVGDDTRGWGPPFAGGESAYFLCT
                     NRNKKSLTVDLKSAGGIALVRQLAERADVLIENFRPGAMERLGLGDQLLREKNPRLVY
                     ASLSGFGADGPMADMPGYDLIVQAWGGLMSVTGSAESGPLKVGVAIIDLVAGLMLGKA
                     IVAALYAREKIGVGQKLDTSLLEAEVAALINAGSNYLIGGQVPGRWGNAHPTIVPYQS
                     FQTADSFLVLGAASETIWQRLCPALSRAELADDPRFAKNADRVENRIELIAMLSEIFK
                     TRTTHDWIAALNAADVPCAPVQTIDQVFAAPQVLHRNMLVEVDHPTAGKVKLAGIPVK
                     FSASPASVRLPPPLLGEHCAEVLSSWLGMSGEEIERLKRMSVL"
     gene            1131..1607
                     /locus_tag="VE170_15795"
     CDS             1131..1607
                     /locus_tag="VE170_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYJ16961.1"
                     /translation="MIGKTFEEFRAGERFSTGRRTITEHDILTFVNLVGLTEPLFLDI
                     EYIRKESLFGERIAPGSLTFGMAEGLTVQTGLIHGTGMAFAGLDKMRLFSPVKVNDTI
                     QVEIEVLDTKPVPARGGGIVRYRQWVKNQRGETVMEYDVARLIRGNEDKPAVKNKQ"
     gene            1665..2627
                     /locus_tag="VE170_15800"
     CDS             1665..2627
                     /locus_tag="VE170_15800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ16962.1"
                     /translation="MSDKQINFGVLLPTREAIMAGRADPSALFQIAEQAEALGFHSVW
                     VGDSLTARPRIDALTTLAAIGARTQRVRLGTAIFLAALRNPILLAYQLASLDWMTGGR
                     IDLGVGYGRPKEPAAEHEFAILGLDPQARMKMSEELVQVMRRLWRENDVSHAGKFSRF
                     DHVTLEPKPTQAGGVPIWLASNAVEPGLKRVARLADGWLNNIKSPELFGECWQKIQEY
                     AAAAGRQSESIHPGIYFTLAAGGKDAVVEGQQFLAQYYNRSYEAVANAMLCVTGSWDE
                     VIDWTEKYVEAGARTVVLRFAANDQLRTLDACADALKRRGLLKL"
     gene            2725..3036
                     /locus_tag="VE170_15805"
     CDS             2725..3036
                     /locus_tag="VE170_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16963.1"
                     /translation="MNSPIRSIQVVAGAIVVSCSMLWAADGIITRSPDASGQSCHLKF
                     PAISEETLFTANPQLKDPKDGDVIDFYGPCNHDPLGKVEVTRQRDDYRRYRNRFDGET
                     D"
     gene            complement(3057..4094)
                     /locus_tag="VE170_15810"
     CDS             complement(3057..4094)
                     /locus_tag="VE170_15810"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ16964.1"
                     /translation="MKAVRMHGYGGAEQLYYESCDAPKITEPHEVIVKLKAAALNHID
                     IWNRMGATGIDVAMPHILGADGAGVVVETGAKVENAQVGAAVCLYPSTGCGLCEFCLT
                     GRDFMCIRLRVLGERLEGTYAQYVKLPGQNCFPTPNGYSLQEAAAFPLVFITLWRMVI
                     TNAALQPGETLLIIGIGGGVASATLQVSKQIGAQVIVTSGSDDKLMRAKSLGADYGIN
                     HQRENFVTAVQSLTDGRGVDVVLDCVGGEVWRQSLASLARGGRLVTCGATAGGQPQDD
                     IAAIVAKQLKIYGSTLGSRREFYQLISFLNASGIKPIIDSVFPLSEAASAQRYLEAAK
                     QFGKVVLEIPD"
     gene            complement(4372..4608)
                     /locus_tag="VE170_15815"
     CDS             complement(4372..4608)
                     /locus_tag="VE170_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16965.1"
                     /translation="MEAMEAVEGLEPVVTNEVSAVYDIRYENATYQAAVCHRCGAKMF
                     PVELLEAHMDRHELKDMFLESELKKLQYAMNRMR"
     gene            4824..5966
                     /locus_tag="VE170_15820"
     CDS             4824..5966
                     /locus_tag="VE170_15820"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ16966.1"
                     /translation="MIEQALKTTAILFTEQKTPVGTLGLIALNNPRALNAMTLEMFQA
                     LEDRLLEWRQRDDIVCVVLHADSEKAFCAGGDVKALVTQLNASADIRPARDFFSHEYF
                     VDYLIHRYPKPLLCWADGITMGGGIGIMNGASCRIVTERTSMAMPEIGIGLFPDVGGS
                     YFLNRMPEGMGMFLGLTSARFDGADAVAIGMADLLIRAERKRAVLDGLVDLPWTREMA
                     NDRELLRDYLRQFADAVEPDQSEVMKRQSVIKEIILQPDIIAIDAAMRRWHGDDEWIS
                     AAIHSYRAGSPTSASVIFEQITGGRGMPLKDVFLREWDMALNFCVGSDFREGVRARLI
                     DKDQQPRWKPPTLGQVDATELSRLFSKQHGQPELLREKLETFAPAH"
BASE COUNT         1274 a         1823 c         1705 g         1219 t
ORIGIN      
        1 ccttactgca ccatgttcct gggcgatctc ggcgccgaag tcgtcaaggt cgagcagccg
       61 ggtgtcggcg acgacacgcg cggctggggg ccgcccttcg ccggcggcga aagcgcttat
      121 tttctctgta ccaatcgcaa caagaaaagc ctcaccgtcg atctcaagtc ggccggcggc
      181 atagcgcttg tccgccagct cgccgagcgc gccgatgtgc tgatcgaaaa ctttcgcccc
      241 ggcgccatgg agcgtctggg cttaggcgac caactgctgc gcgaaaaaaa tccccggctg
      301 gtttacgctt cgctgtcggg gttcggcgct gacggtccga tggcggacat gcctggctat
      361 gatttgatcg tccaagcctg gggcggcttg atgagcgtca ccggcagcgc cgagagcggg
      421 ccgctcaagg tcggcgtcgc gatcatcgac ctggtcgcgg gtttgatgct cggcaaggcg
      481 atcgtcgccg cgctttatgc ccgagaaaaa atcggcgtcg gccaaaagct cgacacctcg
      541 ctgttggaag ccgaagtcgc cgcgttgatc aatgccggca gcaactatct catcggtggc
      601 caagttcccg gcagatgggg caacgcccat ccgaccatcg tgccctacca aagctttcag
      661 accgccgaca gttttctggt gctcggcgcc gccagcgaaa cgatctggca gcgcctctgt
      721 ccggcgctga gccgcgccga actcgccgac gatccacgct tcgctaagaa cgccgaccgc
      781 gttgagaacc gaatagaatt gattgccatg ctctccgaga tttttaagac ccgcacgacc
      841 catgactgga tcgccgccct caacgccgcc gacgtcccct gcgcgccggt gcagaccatc
      901 gatcaagttt ttgccgcgcc gcaagtcctg catcgcaata tgctcgtcga agtcgaccat
      961 cccaccgccg gcaaggtgaa acttgccggt ataccggtaa agttttctgc cagcccggca
     1021 tccgtgcggc tgccgccgcc gctgctgggc gagcactgcg cggaggtctt gtcatcgtgg
     1081 cttggaatga gtggcgaaga gatcgaacga ttgaaacgaa tgagcgtcct atgattggta
     1141 aaacctttga agagttccgc gccggcgagc gcttcagcac cggccggcgg acgattaccg
     1201 agcatgacat cctgaccttt gtgaacttgg tcggcctcac cgaaccgctg tttttggata
     1261 ttgaatacat tcgcaaggaa agtctcttcg gcgagcgcat cgcgccgggc agtctcacct
     1321 tcggcatggc cgaggggctg accgtgcaga ccggtctgat tcacggcacc ggcatggcct
     1381 ttgccggtct cgacaagatg cgcctgttca gcccggtcaa agtcaacgac accattcagg
     1441 tggaaatcga agttttggat accaagcccg tcccggcgcg cggcggcggt atcgtccgct
     1501 accgtcagtg ggtcaaaaat cagcgcggcg aaacggttat ggagtacgat gtcgcgcgat
     1561 tgattcgcgg taacgaagac aagccggccg tgaaaaataa gcagtaacgc attccatatt
     1621 ccccgactgg tgacaagtta ctccgcatag atcgagaaca aacaatgagc gataaacaaa
     1681 taaactttgg cgtgctcctc cccacccgcg aagcgatcat ggccggacgc gccgacccgt
     1741 cggcgctatt tcaaattgcc gagcaagccg aggctttggg ctttcactcg gtgtgggtcg
     1801 gcgatagttt gacggcgcgg ccgcgcatcg atgcgctgac gaccttggcc gccatcggcg
     1861 cgcggacgca gcgggtccgt ttgggtacgg cgatatttct cgccgcgctg cgcaatccaa
     1921 tcctgctcgc ttaccagctc gccagcctcg actggatgac cggcggacgg atcgatctcg
     1981 gcgtcggtta cggccggccc aaggaaccag cggccgaaca tgaatttgca atcctcggcc
     2041 tcgatcccca agcccgcatg aagatgagcg aagagctagt gcaggtcatg cgccggctct
     2101 ggcgcgaaaa cgacgtcagc catgccggca agttcagccg cttcgatcac gtgaccctcg
     2161 agcccaagcc gacgcaggcc ggcggcgtgc cgatctggct ggcgtcgaac gccgtcgaac
     2221 cgggcctgaa gcgcgtcgcc cggctcgccg acggctggct caacaatatc aaaagcccgg
     2281 aactattcgg cgagtgctgg caaaagatcc aagaatatgc cgccgccgcc ggccgccaat
     2341 ccgaatcgat ccatccggga atttatttca ccctcgcggc cggcggcaaa gacgcggtgg
     2401 tcgaaggaca gcagttcttg gcgcaatatt ataaccgctc ctatgaagcg gtggccaatg
     2461 ccatgctttg cgttaccggc tcctgggatg aagtcatcga ttggaccgag aaatatgtcg
     2521 aagccggcgc ccgcaccgta gtcctgcgct tcgccgccaa cgaccagctg cgcaccctcg
     2581 atgcttgcgc cgacgcgctc aagcgccgcg gcttgttaaa gttgtgattg cagacctctt
     2641 tggggcacta ccacccgccc gcgtcctggt ataggatata gagtgccgtc gatcgattgt
     2701 ctctcgatcc gaaaggagca gtttatgaat agtccgattc gatccattca agttgtcgcc
     2761 ggggcgattg tcgtatcctg ttccatgctc tgggcagctg atggaatcat tacacggagt
     2821 cctgatgctt cgggacaatc atgtcatctg aagtttccgg caatcagcga ggaaacgctg
     2881 ttcactgcaa atccccagct caaggatcca aaggatgggg atgtcattga tttttacggt
     2941 ccgtgcaacc atgacccact cggcaaagtg gaagtcaccc ggcagcgcga cgactaccgg
     3001 cgctaccgca atcgctttga tggcgagact gattgagacg cgaagcggcg cctatcttag
     3061 tccggtatct ccaaaacaac cttgccgaac tgcttcgctg cttcgagata acgctgcgcg
     3121 cttgccgcct cactgagagg aaacacgctg tcgatgattg gtttgattcc gctggcattg
     3181 agaaaactga tcagctgata aaattcccgg cggctgccca aggtcgaacc ataaatcttt
     3241 aactgtttgg caacgatcgc cgcgatgtcg tcctgcggct gcccgccggc cgtggcgccg
     3301 cacgtcacca acctgccgcc gcgcgccagc gacgccagac tctgccgcca cacttcaccg
     3361 ccgacacaat cgagcacgac atcgacgccg cgcccatcag tcaacgattg caccgcggta
     3421 acgaagttct ctcgctgatg attgataccg taatcggcgc cgagactttt ggcgcgcatg
     3481 agcttgtcat cgctgcccga ggtgacgatc acctgggcgc cgatctgctt ggatacttgc
     3541 agagttgcgc tcgccacacc gccgccgatg ccgatgatca acagggtctc gcccggctga
     3601 agcgccgcgt tggtaatgac catgcgccac agcgtaatga acaccagcgg aaacgccgcg
     3661 gcttcttgca atgaatatcc gttgggggtc ggaaaacagt tctgtccagg aagcttcaca
     3721 tactgggcgt aggtgccttc gagccgttcg ccgagcacgc gcaaccggat acacatgaaa
     3781 tcccgcccgg taagacaaaa ctcacacagg ccgcagccgg tagacggata gagacaaacc
     3841 gcggcgccga cttgggcgtt ctcaacctta gcgccggtct cgaccaccac accggcgccg
     3901 tcggcaccga gaatatgcgg catcgcgaca tctatgcccg tcgcgcccat gcggttccag
     3961 atatcgatgt gattgagcgc ggcggcctta agcttaacga tgacttcgtg gggttcggtt
     4021 atcttcggcg cgtcgcagct ttcgtagtac agttgctccg ctccgccgta accatgcatg
     4081 cgaacggctt tcaattctat tctcccggga aatcattggt taacaaggcg aaactttgaa
     4141 gtcaatttga ttgaccggcc actaacggcg aaacaccgtg ctacgaacca agggcgaatc
     4201 tgccaacaca gtggcaaatt aaactggcct tgaaatgagg gagatttttc ggggaaaggg
     4261 tgtcccctta tcggggacac cctggggatg atcagcaaac gtgagctgag gaggagccca
     4321 cgcctgcgcc tgggaacaca gttgccggcc gttgccggcg aaatcggcag actaacgcat
     4381 tctattcatt gcgtactgga gtttcttaag ctcgctctcg aggaacatat ctttcagctc
     4441 gtggcggtcc atatgcgctt cgagcaattc caccgggaac atcttagcgc cgcaacggtg
     4501 acaaaccgcc gcctgataag tcgcgttttc gtagcgaata tcgtaaactg cgctgacttc
     4561 attggtgacc acgggttcca aaccttctac agcttccata gcttccatgt tgtccctctc
     4621 tgtcttcaaa ttacggctcg attgctctgt acagcaatca atatgccagc cccgcgagcc
     4681 gcttgcggct gcgtcaagtc gttgattttc caccgaatta cgataccgtg tgaatattgt
     4741 tcagacatcg gacggacaca aaagacgcaa gcccctggca atgacaacaa ttttgttccg
     4801 cccgaccccg gagccgcgca actatgattg agcaagccct gaaaaccacc gctatattgt
     4861 tcaccgagca gaaaacgccg gtcggcaccc ttgggctgat cgccctaaac aatccgcggg
     4921 cgctcaacgc gatgaccctg gagatgtttc aggccttgga agaccgactg ctggaatggc
     4981 gccagcgcga tgatatcgtg tgcgtcgtcc tgcacgcaga ttcggaaaaa gccttttgtg
     5041 ccggcggcga cgtcaaagcc ctggtaacgc agctgaatgc cagtgccgac atccggcccg
     5101 caagggattt tttctcccat gaatatttcg tcgattatct aattcaccgc tatccgaaac
     5161 cgctgctttg ttgggccgac ggcatcacca tgggcggcgg catcggcatc atgaacggcg
     5221 cctcctgccg gattgtcacg gagcgaactt cgatggccat gccggagatc ggcatcggtc
     5281 tatttcccga tgtcggtgga agttattttt tgaatcgcat gccggaggga atgggcatgt
     5341 tcttgggtct gacttcggcg cgcttcgacg gcgccgacgc cgtcgcgatc ggcatggccg
     5401 acctgctcat ccgcgccgag cgcaagcgcg cagttctcga cggcctggtc gatttgccat
     5461 ggacccggga aatggccaac gaccgtgaat tattgcgcga ctatttgcgc cagttcgcgg
     5521 acgccgtcga gcccgaccaa tccgaggtca tgaaacgcca gtctgtcatc aaagaaataa
     5581 tcctgcagcc ggacatcatc gccatcgacg ctgccatgcg ccgatggcat ggcgacgacg
     5641 aatggataag cgccgcgatt cacagctacc gcgccggctc accgacctca gcatcggtaa
     5701 tttttgaaca gatcaccggc ggccggggta tgcctttgaa agatgtattc ctgcgcgaat
     5761 gggacatggc gctcaatttc tgcgtcggtt cggacttccg cgaaggtgtg cgcgcgcgcc
     5821 tgatcgacaa agatcaacag ccgcgctgga agccgcctac cttgggtcaa gtcgatgcca
     5881 cggaactgag ccggttgttc tcaaagcagc atggccaacc ggagctattg agagagaagc
     5941 tcgaaacctt cgcgccagcg cactgatctc acgatcaatc tttcggatta ctttggttgc
     6001 gcaaaatctc gactatgcat g
//
LOCUS       DASQKS010000616         9528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1600069, whole genome shotgun sequence.
ACCESSION   DASQKS010000616 DASQKS010000000
VERSION     DASQKS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600069"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..786
                     /gene="rph"
                     /locus_tag="VE170_15825"
     CDS             64..786
                     /gene="rph"
                     /locus_tag="VE170_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYJ16967.1"
                     /translation="MRIDGRKQNQLRTLRITPSYIKTADGSVLIEMGDTKVICTAKLE
                     ERVPPFLRNSGKGWITAEYGMLPGSSQQRIGRESSRGKVGGRTHEIQRLIGRSLRAVA
                     DLKILGERTIWIDCDVIQADGGTRTASITGAYVAVAEAVKSWLDKGKLLQTPIRDAVA
                     AVSIGIVEGRILLDLCYLEDSVADVDMNFVMTGSSKFIEVQGTAESAPFTRKQMERMA
                     EIAQDGIKELLKAQKQAISGLA"
     gene            825..1445
                     /locus_tag="VE170_15830"
     CDS             825..1445
                     /locus_tag="VE170_15830"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HYJ16968.1"
                     /translation="MIELLVATTNSGKFAEVQAFLGPLPIRIRSLGDLNDPPGVVEDG
                     ATFEANALKKARTLADFSGLLTVADDSGLEVDALGGAPGIYSARYCGREGNDDENNRK
                     LLRELTGVAEAKRTARFVCALALCAPAGLAIADWTARESCAGLITFELTGMNGFGYDP
                     LFFYVPFGKTFGEIGRDLKATVSHRGKALKKLAQVLPQLVDLAVQP"
     gene            1488..1564
                     /locus_tag="VE170_15835"
     tRNA            1488..1564
                     /locus_tag="VE170_15835"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1522..1524,aa:Pro,seq:tgg)
     gene            1655..1731
                     /locus_tag="VE170_15840"
     tRNA            1655..1731
                     /locus_tag="VE170_15840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1744..1820
                     /locus_tag="VE170_15845"
     tRNA            1744..1820
                     /locus_tag="VE170_15845"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1778..1780,aa:His,seq:gtg)
     gene            1899..1974
                     /locus_tag="VE170_15850"
     tRNA            1899..1974
                     /locus_tag="VE170_15850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1932..1934,aa:Lys,seq:ctt)
     gene            2146..2664
                     /locus_tag="VE170_15855"
     CDS             2146..2664
                     /locus_tag="VE170_15855"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16969.1"
                     /translation="MEQSLSYTMPMEVHLPAALVRGVLETNQDRLSNHLLVRQNDQVF
                     SLRDATVEDLTGKLIASRTNEYVVYMREVFLIADLSPAGQSQRSGLEGLYRKKNTSKA
                     LISVGPYLIQGDVYLVPGGALYDLLLEKNQFIPLTNATILSRQPASPRTYLINRHKIG
                     FMTAIGDGLVEL"
     gene            2667..2987
                     /locus_tag="VE170_15860"
     CDS             2667..2987
                     /locus_tag="VE170_15860"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HYJ16970.1"
                     /translation="MDAYPTEINHLKARGVVRITWNDGHIGEYEEPYLRGYCPCAMCQ
                     GHGSQRQFISVPEAKLQEIHGVGNYAIEFRWHDGHSTGIYTFEYLRSLCPCTACAGTH
                     SGVS"
     gene            3498..3580
                     /locus_tag="VE170_15865"
     tRNA            3498..3580
                     /locus_tag="VE170_15865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3532..3534,aa:Leu,seq:tag)
     gene            3724..5049
                     /gene="tig"
                     /locus_tag="VE170_15870"
     CDS             3724..5049
                     /gene="tig"
                     /locus_tag="VE170_15870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYJ16971.1"
                     /translation="MKVDIDELSPIQRKVSIELPADRVAQEFSHAYKNLGQRVTVKGF
                     RTGKIPRGVLQGIYGEDIKGEVRSHLVEESLAEVVKERDLKIVSRPEVEAGEIKEDGE
                     FSFSAVFEIKPAIELKNYLGVEVEKIKVAISEEQVDEALKRLQEGHARLELIEGRDVV
                     QKGDFITVDFEGTIAGKPFAGGKGENYVLEVGAGQTLPQFEEAVVGLQLGQQHSVPVT
                     YPDSYPNAEIAGKAVEFALTVREIKQKVLPVLDDDFAKDHGEHGSLAELRAAIRRRLE
                     DELKHYQDEDLKEKIISRLIEAHPFNAPPSMIERQTRYLMERYQSQIAGHQSGAEPPA
                     PPMEEARKTFEGRAARQVQATLLVERIAQAETIEVSDKDVQERVDNLARSAGDRAKGV
                     REYYARPDARDDLRAQIAFDRTLSFLLERAEIKEVDAPVSPVDAEPEKS"
     gene            5083..5685
                     /gene="clpP"
                     /locus_tag="VE170_15875"
     CDS             5083..5685
                     /gene="clpP"
                     /locus_tag="VE170_15875"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HYJ16972.1"
                     /translation="MNFIPMVVEQTGRGERAYDIYSRLLKERIVFLGSVVNDEVANVI
                     TAQLLFLESEDPEREIFFYINSPGGSVTAGLAIYDTMQYVRPQISTLCVGQAASMGAV
                     LLAAGTKGKRFALPHSRIMIHQPLGGFEGQATDIEIHAREILRMREELNNILMKHTGQ
                     GLKKIEKDTDRDMFLTGKQAQEYGIVDDVIAARPDNALKK"
     gene            5710..6969
                     /gene="clpX"
                     /locus_tag="VE170_15880"
     CDS             5710..6969
                     /gene="clpX"
                     /locus_tag="VE170_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HYJ16973.1"
                     /translation="MPKHGEHGSGNLVCSFCGKSQDEVRKLIAGPTVYICDECIDLCN
                     DIIAEEIDHDENLSSSSNVPKPAEIKRVLDQYVIGQERAKKILAVAVHNHYKRIDSQM
                     VTGDVELQKSNILLLGPTGSGKTLLAQTLARFLQVPFTIADATSLTEAGYVGEDVENI
                     ILNLLQAAEYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIIEGTM
                     ASVPPKGGRKHPQQEFLQVDTTNILFICGGAFVGLEDIIRKRTGRKRMGFGGQLEDSE
                     KIGQRASDLLHSVQTEDLLKFGLIPEFIGRLPVIATLDELDDSALMRILKEPKNALTK
                     QYSKLFEMESVHLKFTEDALRAIAREAMKRKSGARGLRAIMENIMLDIMYDMPSQPNI
                     KEVVISEDVVEKHEPPIVVYTKAAESA"
     gene            6981..9443
                     /gene="lon"
                     /locus_tag="VE170_15885"
     CDS             6981..9443
                     /gene="lon"
                     /locus_tag="VE170_15885"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HYJ16974.1"
                     /translation="MLFRNDKKDSETTAGGTRVPLLPLRDIIVFPHMVVPLFVGRQRS
                     IKALEEATQKQSLIFLSSQKDAKTNEPSEDDIYRIGTLGSVVQMLKLPDGTVKVLIEG
                     KKRAKIASFAVNPDFFLVDVEDAQEVNDRTTEAEALIREVHTTFENYVKLKKKIPPEM
                     VMSVSSIDEPGRLSDTIVAHLGIKLEDRQNLLETFNAAERLEKVLGHMRAEIEILEVE
                     RRIRSRVKKQMERSQKEYYLNEQMRAIQKELGEKDEFKNEIQEIEEKLKQKKLSAEAK
                     EKVEKELKKLKMMSPMSAEATVVRNYIDWIISLPWNEFTDDKLDINEAEKVLDEDHFG
                     LEKVKERILEYLAVQSLVGKIKGPILCLVGPPGVGKTSLGRSIARATGRKFIRVSLGG
                     VRDEAEIRGHRRTYIGALPGKIVQSMKKAGSSNPVFLMDEIDKMSTDFRGDPSSALLE
                     VLDPEQNTAFNDHYLDLDFDLSKVMFITTANSLDRIPRPLQDRMEIIRIAGYTELEKV
                     SIAKKYLIEKQREANGLTPENIVFTDNALMGVVRHYTKEAGVRSLEREIAAVCRKVAV
                     EVVKKDRNSHVQVGTKSLHKFLGPIKFRYGKADAETKVGVTTGLAWTEMGGELLSTEV
                     TVMPGKGQLIITGKLGDVMQESAQAAMSYVRSRATALGLDKDFYQKLDIHLHVPEGAI
                     PKDGPSAGITMATSLVSALIKIPVRNDLAMTGEITLRGTVLPIGGLKEKVLAAHRAGI
                     KKILIPADNEKDIEEIPASVLKSLDLELVAHVDEVLKKALVLNDPETLFSHTPVEIQP
                     KDENPPFSDKVEEVPVSEILPQ"
BASE COUNT         2378 a         2345 c         2651 g         2154 t
ORIGIN      
        1 cggccttagg cggcgacggc aagcaattga gaaggaagaa atcacaataa gatgattgca
       61 aaattgcgaa tcgacggacg gaaacagaat caattacgaa ctttgaggat caccccgagc
      121 tacataaaga cggccgacgg ctcggtgctg atcgagatgg gcgataccaa agtaatttgc
      181 acggccaagc tcgaagagcg cgtgccgccg tttcttcgaa acagcggcaa gggttggatc
      241 accgccgagt acggcatgtt gccggggtcg tcccagcagc gcatcggccg cgagtcctcg
      301 cgcggcaagg tcggtggccg cacccatgaa atacaacgct tgatcggtcg cagtttgcgg
      361 gcggtcgccg acttgaaaat tctcggcgag cgaaccattt ggatcgactg cgacgtcatt
      421 caagccgacg gtggcacgcg tacggcgtcg atcaccggag cttatgtggc ggtggcggaa
      481 gcggtcaaga gttggctcga caaaggcaag ctgttgcaaa ccccgataag ggatgcagtc
      541 gcggcggtga gtatcggcat cgtcgaaggg cgaattttat tagacctatg ctacctcgaa
      601 gacagcgtag ccgatgtcga catgaacttc gtcatgaccg gctcgagcaa atttatcgaa
      661 gtccaaggca ccgccgaaag cgcaccgttc acgcgcaagc agatggagcg catggcggaa
      721 attgcccaag acggcatcaa agagttgctc aaagcgcaga agcaagcgat ttctggcctg
      781 gcttaagata aattttatcg gcagtaccca aagacattct tgccatgatc gaactcctcg
      841 ttgccacgac taactctggc aagtttgcag aggtgcaagc gttcctcggc ccactgccga
      901 ttcgaatccg ttctttaggt gatctcaacg atcctcctgg agtggtggaa gatggcgcga
      961 ccttcgaagc aaatgcgcta aaaaaagcgc gcaccctggc cgatttttcc gggttgctaa
     1021 cggtagccga tgattccggt ttagaagtcg atgcgctggg cggagcgcca ggaatttatt
     1081 cggcgcgcta ctgcggcagg gaaggcaacg acgatgaaaa taatcgcaag ctcctgcgcg
     1141 aattgaccgg cgtggccgaa gcgaagcgaa ccgcgcgctt cgtctgtgca ttggcgcttt
     1201 gtgcacctgc gggccttgcc attgctgatt ggaccgcgcg cgaatcttgt gccgggctaa
     1261 tcaccttcga actaacaggt atgaatggtt tcggttatga tccgttgttt ttctatgttc
     1321 cgtttggcaa aaccttcggc gaaattggcc gcgaccttaa agcgactgtc agtcaccgcg
     1381 ggaaggctct gaaaaaactt gcgcaagtat tgccgcagct agttgatttg gcggttcaac
     1441 cttgatttga tcgaagccca gtgtagaatg gccgctcggt tgaatgacgg ggcgtagcgc
     1501 agcctggtag cgcacctgcc ttgggcgcag ggggtcggaa gttcgaatct tctcgccccg
     1561 accaacgaaa aatggtatgt gctgagctat gagtactgag tactgtaggg tagaggcttc
     1621 tcccttcagt actcagtaat cgctattcga atgggcgcca gtagctcagc tggatagagc
     1681 aacggccttc taagccgtag gtcatgggtt cgaatccctt ctggcgcacc agactgtttt
     1741 gcggtgagtg tagctcaagt ggttagagca ctggattgtg gttccagggg ttgagggttc
     1801 aagtcccttc actcacccca gtttcaattc ggcgaagatc gaaggcggat agtcgcaatc
     1861 cttagggatt tatctgccac attagatttg atcggtgcgg gccgctagct cagttggtag
     1921 agcagctgac tcttaatcag cgggtccgga gttcgagtct ccggcggccc accaaattct
     1981 ttcttctgtc tcaccgttgg tgggtttaca cccaggccgg ttcaatcatc gatcatagat
     2041 tggacgaaaa tcactggacc ttaagggtcc gcagcagtat gctgtaaatt aaaactgatt
     2101 cgactgtttt gatctgacaa caatccgcga aacactctgg cagccatgga acaaagtctg
     2161 agttatacga tgccgatgga ggttcacctt ccagcggcac tggtgcgcgg cgtacttgag
     2221 accaatcaag atcggctcag caatcatctc ctagttcgcc agaatgatca ggttttctcg
     2281 ttgcgggatg cgactgtaga agacctcaca ggcaaactga tcgccagccg caccaacgaa
     2341 tatgtcgtct atatgcgtga ggtttttttg atcgccgacc tcagtccggc cggccagtcc
     2401 cagcgctccg gtctcgaggg tctgtatcgt aaaaaaaata ccagtaaggc attgatcagc
     2461 gtcgggccgt acttgatcca gggcgacgtt tatctggttc ccggcggcgc gttgtacgat
     2521 ctgctgctcg aaaaaaatca attcataccg ttgaccaatg cgactattct cagtcggcaa
     2581 cccgcctcgc cccggactta cttgatcaat cgtcataaga tcggctttat gacagccatc
     2641 ggcgacggtt tggtggagtt atgaccgtgg acgcttatcc aactgaaata aatcatttga
     2701 aagcgcgcgg tgtggtgcgc ataacctgga atgacggcca tattggcgaa tatgaagagc
     2761 cttatctacg cggttattgc ccctgtgcga tgtgtcaggg ccatggctct caacgccaat
     2821 tcatttccgt acccgaagcg aagcttcagg agattcacgg cgtcggcaat tatgccatcg
     2881 aattccgttg gcatgatggc cacagcaccg gaatctatac tttcgaatat ctgcggtcgc
     2941 tttgcccctg caccgcctgc gctgggacgc acagtggcgt ctcgtagttc gtgtcggccc
     3001 cgagagttgc tatccgtact tccctttact agctgacctc gctccgcggt tagtttcaca
     3061 gcgcggcccg catttatttc cttttgtgac ccgccttttc ttgactctcc acagttcgtc
     3121 gctttaccgg ttgcttcggt aagcttttaa attgatattg ttgagccgct ttggcgcgat
     3181 tatttgcacc gctagtatct ccgtcagagt tctgtgcagc ggcatgcagc aaacaagttt
     3241 acgacgcagg tcgctggtgc ggggcattcc caaaagtacg actcggactg agcgcctttt
     3301 gtcaatattc ataccgcacg caactgtact gtcgcgttat taagaatgct gaatgccaag
     3361 tgaatttggg gatggattag ttctatccaa atacctggca gttacgctcg gtacgcttga
     3421 cttaccaccg ggtaattctt actctccttt cctagtcggc gaagtaggat tgacaggttg
     3481 cagcgaccag aagaagtgcg agagtggcgg aattggcaga cgcactagac ttaggatcta
     3541 gcgggcaacc gtgggggttc gagtcccccc tctcgcacca agaaagggtt ttcgcttcgt
     3601 tcgatatgca ccgagataga aaggttgatg gtaaggctac cgcccaactc tcattgcgag
     3661 atcccgtgta gttcgggctc gtcctgtgtc aaagcgcaaa cgcctctgtc gcaagaaaaa
     3721 tagatgaaag tcgatatcga tgaattgagt ccgatccaac gcaaggtcag catcgaattg
     3781 ccggcggatc gggtcgccca agaattttcc catgcgtata aaaacctcgg ccagcgcgtg
     3841 accgttaaag gctttcgcac cggcaagatt ccgcgcggcg tgcttcaagg aatttatggt
     3901 gaggacatca aaggcgaagt acggtcgcat ctcgtggaag aatctctggc ggaggtggtc
     3961 aaagaacgag atttgaaaat cgtctcacgg ccggaagtgg aggccggcga aattaaggaa
     4021 gacggagagt tttccttctc ggcggtcttc gagatcaagc cggccatcga attgaaaaat
     4081 tatctgggtg ttgaagttga aaagatcaag gtcgccatca gcgaagagca ggtcgatgag
     4141 gcacttaaac gtttgcaaga aggccatgcc cgcctagagc tgatcgaagg ccgcgatgtc
     4201 gtccagaagg gcgattttat taccgtcgat ttcgaaggca ccatcgccgg caagccattt
     4261 gccggcggca aaggggagaa ctacgtcctc gaagttggcg ccggccagac gttgccacaa
     4321 ttcgaagagg ccgtagttgg attgcagttg ggtcagcagc actcggttcc ggtgacgtat
     4381 ccggacagct atccaaatgc tgaaatcgcc ggaaaggcgg tcgagtttgc ccttaccgtt
     4441 cgtgagataa aacagaaagt actgccggtg cttgacgatg atttcgccaa agatcacggc
     4501 gagcatggct cgttggcaga gctgcgagcg gcgattcgtc ggcggcttga ggatgaactc
     4561 aagcattacc aggacgaaga tcttaaagaa aaaattatca gccgtttgat cgaggcgcac
     4621 ccgttcaacg ccccgccgtc gatgatcgaa cggcagactc gctatcttat ggagcgctac
     4681 caaagccaaa tagccggaca ccagagtggg gcagagccgc ccgcgccccc catggaagag
     4741 gcgcgcaaga ccttcgaagg ccgcgccgct cgtcaggtac aggcgacgtt actcgtcgaa
     4801 agaatcgccc aggcggagac tatcgaagtg tcggacaaag acgtgcaaga acgggtcgat
     4861 aatctagcac gtagcgccgg tgaccgcgcc aaaggcgtgc gggaatacta tgcgcgcccc
     4921 gacgctcgtg atgatttacg cgcgcaaata gcgtttgatc gcactcttag cttcttgctt
     4981 gagcgggctg aaataaaaga ggtcgacgcc ccggtatctc cagttgatgc tgagcccgaa
     5041 aaaagctaga atgctcttaa tgattgaggg gtttacccac atatgaactt catccccatg
     5101 gtcgtcgagc aaaccgggag aggcgaacgg gcatatgaca tttattcgcg tctgctaaaa
     5161 gagcgaatcg tatttctggg ctcggtggtc aatgatgagg tggccaacgt cattaccgcg
     5221 caactgctgt tcctcgaatc cgaagatcct gagcgagaaa ttttctttta catcaatagt
     5281 ccgggtggat cggttactgc tggcttggcg atttatgaca ccatgcagta tgtgcggccg
     5341 caaatttcga cgctttgcgt cgggcaagcg gcaagcatgg gtgctgtatt gctagccgcc
     5401 ggcacgaaag gcaaacgctt tgcgctgcca cattcgcgga tcatgattca tcaaccgctc
     5461 ggcggtttcg agggccaggc gaccgacatc gagattcacg cccgtgaaat tcttcgtatg
     5521 cgtgaggaat taaacaacat cctcatgaaa cataccggcc aggggctgaa gaaaatcgaa
     5581 aaagacacag atcgcgacat gttcttgacg gggaagcagg ctcaggagta tggcatagtt
     5641 gacgatgtga tcgcggcgcg tcctgacaat gcgctcaaga aatagctcgt agtttggctt
     5701 ggggggtcta tgcctaaaca tggtgagcac ggatcgggaa atctcgtctg ttctttttgc
     5761 ggcaaaagcc aagacgaggt tcgcaagctc atcgccggtc cgacggttta catttgcgat
     5821 gaatgcatcg acttatgtaa tgatattatc gctgaagaaa tcgaccacga tgaaaaccta
     5881 agttcgtcgt caaacgttcc gaaacccgcg gaaatcaagc gggtgctcga ccagtatgtg
     5941 atcggccaag agcgggccaa gaagatcttg gcggtcgccg tccacaatca ttacaaacgc
     6001 atcgacagcc agatggtgac cggggatgtc gagctgcaga aaagcaacat tctgctgctt
     6061 gggcccactg gatcgggcaa aaccctcctc gcgcagactc ttgcccgttt tctgcaagtt
     6121 ccctttacca tcgccgacgc cacaagcctg acggaagcag gttatgtcgg cgaggatgtg
     6181 gagaacatca ttctcaattt gttgcaagcg gccgagtatg acgttgaaaa ggcccagcgc
     6241 ggtatagtct atatcgacga gatcgacaaa atttcgcgta aatcggacaa tccctcgatt
     6301 acccgagatg tgtccggcga aggggtgcaa caggcattac ttaagatcat tgaaggcacc
     6361 atggcgagcg tgccccccaa gggcggacgc aaacatcctc agcaggaatt cttgcaggtg
     6421 gacactacca atattctatt tatttgcggt ggggctttcg tcggtcttga agatatcatt
     6481 cgtaagcgga cggggcgcaa gcggatgggc tttggcggac aattggagga cagcgaaaaa
     6541 atcggccaac gggcttcaga tttgctgcac agcgtccaga cggaggattt actcaagttt
     6601 ggcttaattc ctgagttcat aggcagatta ccggttatcg cgactctcga tgaattggat
     6661 gacagcgccc tgatgcgaat actaaaggaa cccaagaatg ctttgaccaa gcagtattcc
     6721 aagttatttg agatggaatc ggttcacctc aagtttacag aggatgcgtt gcgagcgatt
     6781 gcacgcgaag ccatgaaacg taaatccgga gcgcgcgggc tacgcgcgat catggaaaat
     6841 ataatgttgg atatcatgta cgacatgcca tcgcagccca atattaaaga ggttgtgatt
     6901 tcagaggatg ttgttgaaaa gcatgaacct ccgatcgtcg tatacaccaa ggctgcagag
     6961 agcgcttagg aggggaccgt atgttgtttc ggaatgacaa gaaagattca gaaaccactg
     7021 ctgggggcac aagagtgccg ttgctgccat tgcgggatat tattgttttc ccacacatgg
     7081 ttgtgccgct atttgtcggg cgccaacgct cgatcaaggc gctcgaagag gcgactcaga
     7141 agcagagtct tattttctta tcctcgcaga aggatgctaa aaccaatgag cccagcgaag
     7201 acgatattta ccgtattggt actcttggct cggtggtaca gatgctcaag ctccccgatg
     7261 gcacagttaa ggttctgatc gaggggaaaa agcgtgccaa aatcgccagc tttgccgtca
     7321 atccagattt ttttctggtt gatgtggaag atgctcaaga ggtgaacgat cgcaccacgg
     7381 aggcggaggc gttaatccga gaagttcaca ccacctttga aaattacgtc aagttaaaaa
     7441 agaaaatccc tccggaaatg gtcatgtcgg tgtcctccat cgatgagccg gggcggctct
     7501 ccgatactat agttgcccat cttggcatca agctagaaga taggcagaac ttgctggaga
     7561 ctttcaatgc cgcggaacgt cttgagaagg ttctcggcca tatgcgcgcc gaaatcgaaa
     7621 ttcttgaagt cgagcggcgt attcgctcgc gcgtcaagaa gcaaatggag cgctcccaga
     7681 aggaatacta tctcaacgaa cagatgcggg cgatacaaaa agagctgggt gagaaggatg
     7741 agtttaagaa tgagattcag gagatcgagg aaaagttaaa acagaaaaag ctttccgccg
     7801 aagcgaaaga aaaagttgag aaggaactga agaagctcaa gatgatgtcg cctatgtcgg
     7861 cggaagcaac ggtggtcagg aattatatcg attggattat cagtttgccg tggaatgaat
     7921 tcaccgacga caaactcgat atcaatgagg ccgaaaaggt tttggacgaa gatcattttg
     7981 gactggaaaa ggtcaaagag cgcattctgg aatatctcgc cgtccaaagt ttggtgggca
     8041 aaattaaagg tccgatactc tgtttggttg ggcctccggg cgtcggcaaa acctcgctgg
     8101 gtcgatcgat cgcgcgcgca accggccgta aatttatccg ggtgtccctc ggcggcgtcc
     8161 gtgacgaagc ggaaatccga ggtcatcgcc gtacctacat cggcgcattg ccgggtaaaa
     8221 ttgttcaatc catgaagaag gctggatcga gtaacccggt atttttgatg gacgaaatcg
     8281 acaaaatgtc tactgatttc cgcggcgatc cttcttcggc gctattggaa gtgcttgacc
     8341 cggaacagaa tacggcattc aacgatcact acctcgatct cgatttcgat ctttcgaagg
     8401 tcatgtttat caccactgcc aattcgctgg atcgcattcc gcggccgttg caagatcgga
     8461 tggagatcat tcgaattgcc ggttacaccg aattggaaaa ggtcagcatc gcaaagaaat
     8521 acctgatcga gaagcaacgc gaagctaacg gcttaactcc ggagaatatc gtctttaccg
     8581 acaacgcatt gatgggtgtc gtgcgccact atactaagga agccggcgtg cgtagccttg
     8641 aacgagaaat cgccgcggtt tgccgcaagg tagcggtgga agtcgtcaag aaagaccgca
     8701 actctcacgt tcaagtcggc accaagagcc tgcacaagtt tctcggcccg atcaaattcc
     8761 gttacggtaa agcggatgca gaaaccaaag tcggagttac taccggcctg gcttggacag
     8821 aaatgggcgg cgagttgctc tcgactgagg tgaccgtaat gccgggaaaa gggcaactga
     8881 ttattaccgg taagctgggc gacgttatgc aggaatccgc acaggcggcc atgagttacg
     8941 ttcgctcccg cgcgactgcc ttgggattag ataaagattt ttatcagaag ctggatattc
     9001 atcttcacgt acccgaaggg gcaattccca aagatggtcc ttccgccggt atcaccatgg
     9061 ctacctcctt ggtatcggca ttgataaaaa ttccagtgcg caacgacctg gctatgaccg
     9121 gtgaaatcac attacgcggt acggtgttgc cgattggcgg tcttaaagaa aaagttttgg
     9181 cggcgcatcg ggccggaatc aagaaaatcc tgattccggc agacaacgaa aaggatatag
     9241 aggagattcc cgccagtgtg ctcaagagcc tggatttgga gctcgttgct catgtcgacg
     9301 aagttctcaa gaaggcgttg gtgttgaacg atccggagac tttgttcagc cacacgccgg
     9361 ttgaaatcca accgaaagac gagaatccgc ccttctctga taaagtagaa gaagtacccg
     9421 taagcgaaat tcttccgcaa tagttgacgg ccaattggcg cgggaatgcc aggttatttg
     9481 acacccggca ttcccgctgt tataagtcca cattgccaat tatcggcg
//
LOCUS       DASQKS010000617         6477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3720989, whole genome shotgun sequence.
ACCESSION   DASQKS010000617 DASQKS010000000
VERSION     DASQKS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720989"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VE170_15890"
     CDS             complement(<1..574)
                     /locus_tag="VE170_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ16975.1"
                     /translation="MAKALDGIRILDMTHVQSGPTCTQILAWFGADVIKVELPGRGDI
                     TRGQLRDKANVDSLYFTMLNSNKRSITINTKHEKGKEVFRKLIKACDVMVENFAPGAL
                     DRQGFTWETIHALNPRLIYASVKGFGPGPYEDCKVYENIAQCTGGSASTTGFEDGPPL
                     VTGAQIGDSGTGIHLVAGILAALFQREKSGQ"
     gene            complement(704..2419)
                     /locus_tag="VE170_15895"
     CDS             complement(704..2419)
                     /locus_tag="VE170_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ16976.1"
                     /translation="MATTANEGKKEATGPVISGGHLVAKALKNEGVEVIWTLCGGHII
                     DIYDGCIDEGIKIIDVRHEQVAAHAADGYSRQSGKIGCVVTTAGPGTTNAMTGIANAF
                     RAESPLVMIGGQGALSQHKMGSLQDLPHVDIMAPITKFAASVPHTERVADMVSMAFRE
                     AVNGAPGPSYLEIPRDVLDAKVPLERARIPKPGAYRASTKSIGDPADIEKLADILVNS
                     ERPCALFGTQVWTSRGHDDAVSLCRALNMPGYMNGSSRGIFAPEDPHGFNRTRRMAFD
                     KADVILIVGCPFDFRLGYGRRLRADATVVQIDLDYRNVGKNRDISLGLVGDCGAILKA
                     VEQAASGRVSKGNAQRAEWMKELRAAEAKAYEKLLPSFKSESTPINPYRVAYEINEFL
                     TDNTVYIGDGGDVVTITATAVEPRKAGNWMDPGPLGTLGVGTSFSMATKFVHPQKEVL
                     CYYGDGSFGMTAWDMETAQRFKLPYMAVIGNNSAMNQIRCGQIGKYGPERGCVGNKLG
                     DVDFEKFADCFGGWGIAVREPSQIRPALEKARERVAGGQCAIVNIWVDINEYAPGTKA
                     QTMYK"
     gene            complement(2651..3271)
                     /gene="recR"
                     /locus_tag="VE170_15900"
     CDS             complement(2651..3271)
                     /gene="recR"
                     /locus_tag="VE170_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYJ16977.1"
                     /translation="MSMYPAPLARLIQELSKLPGVGEKSAARLAFHMLKGKRDDVFRL
                     ADSIGRLRQEMGLCDRCFGFSEVHAQNEDGTQCDVCRNADREEDKICVVEEPADMIAV
                     EKSQEYHGQYHVLHGTISPLDGVGPDALRIKELLERLRGGAVKEVIVATNPTMDGEAT
                     AFYLSKLIKPLGVSVTRIARGLPMGGDLEYTDAVTLGKALEGRREI"
     gene            complement(3363..3686)
                     /locus_tag="VE170_15905"
     CDS             complement(3363..3686)
                     /locus_tag="VE170_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYJ16978.1"
                     /translation="MASIPGMGNLLKQAQDMQARMAKVQEELAHKTVDGAAGGGMVQV
                     TVNGQFNLTAVKIEPSVISGEDKGMLEDLVLAAVNDGMRKAREMASAEMAKLTGGFKI
                     PGLMP"
     gene            complement(3728..5419)
                     /gene="dnaX"
                     /locus_tag="VE170_15910"
     CDS             complement(3728..5419)
                     /gene="dnaX"
                     /locus_tag="VE170_15910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYJ16979.1"
                     /translation="MSYLVLARKWRPQNFDDVAGQEPITRTLKNAIKANRIAHAYLFT
                     GVRGVGKTTAARILAKALNCERGPTATPCNQCSQCEEITSGSSIDVLEIDGASNRGID
                     EIRQIIENVRYQPAKCRFKIYIIDEVHQVTKDAFNALLKTLEEPPPSVKFILATTEPQ
                     RLPETILSRCQRYDFRRISLREIVERLAAIGKSEGLRITDEALVLLAREADGSMRDAQ
                     SLLEQVLASAMPAVGDEQQATVDATTLQEVVGLAPRQSLYQLSQAVLDGDPRRCVELI
                     AEVMHQGRDLSRLSRDLVEHFRNLLVARLTESRMGAARVGGADMAQLLDLPDQEIADL
                     VRQSSHVSVELLLDYLEFIAAGDEDVARSATPRFALEAVLIRLANLPQALPVTEILSR
                     LERLEGRISGEVRSPVAAERPRAVPVAAPVVIKAPVTMSPLASIPAADDDVSKWREFI
                     AFVGKEKKLLASHLGASAALSLPPAPLKVSVAERHHLGFLNDADNLAALGDLAKRFFG
                     VDAIIQFVAAAGEVAPPTSFDAARAEGAAEDRSEMVKEALRIFGGSVRSVRRENP"
     gene            complement(5530..5627)
                     /gene="ffs"
                     /locus_tag="VE170_15915"
     ncRNA           complement(5530..5627)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VE170_15915"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5779..5869)
                     /locus_tag="VE170_15920"
     tRNA            complement(5779..5869)
                     /locus_tag="VE170_15920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5833..5835),aa:Ser,seq:gga)
BASE COUNT         1332 a         1960 c         1795 g         1390 t
ORIGIN      
        1 cctggccgct cttctcgcgc tgaaaaagcg ccgcaagtat gccagcgacg aggtgaatac
       61 cggtgccgga atcgccgatc tgcgcgccgg tgaccagcgg cggaccatct tcgaatcccg
      121 tggtgctcgc cgagccgccg gtgcactggg cgatgttttc atagaccttg cagtcttcgt
      181 aggggccggg gccaaaacct ttcaccgaag cgtagatcag gcgcgggttg agggcgtgaa
      241 tcgtttccca ggtaaaccct tgacgatcga gcgcgccggg cgcgaagttc tcgaccatca
      301 cgtcgcaagc cttgatcagc ttgcgaaaca cttccttgcc tttttcatgc ttggtgttga
      361 tcgtgatgct gcgcttgttg ctgttgagca tggtgaaata caggctgtcg acattggcct
      421 tgtcgcgcag ctggccgcgg gtgatatcgc cgcggcctgg cagctcgacc ttgatcacgt
      481 cggcgccgaa ccaagcgagt atttgcgtgc aagtcgggcc cgactgcaca tgggtcatgt
      541 ccaatatacg aattccgtcg agtgcctttg ccatcgatct gttttctcct tacatgaatc
      601 tagaaagaaa aagtctcacc gcgcgggaca gtgtacacag accagtaaag atttatcgaa
      661 accctctttt ttgcgttctt tgcggcaaaa tctcttcacc gccttacttg tacatcgtct
      721 gcgctttggt gccgggcgcg tattcgttga tgtcgaccca gatgttgacg atggcgcatt
      781 ggccgccggc gacgcgctcc cgggcctttt ccaacgccgg acggatttgg ctcggctcgc
      841 gcacagcgat gccccaaccg ccgaagcagt cggcgaactt ttcaaaatcg acatcgccca
      901 gcttattacc gacgcagccg cgctctggac cgtatttgcc gatctggccg cagcggattt
      961 gattcatcgc tgagttgttg ccgatcaccg ccatataggg aagcttgaag cgttgggcgg
     1021 tttccatgtc ccaagcggtc atgccgaagg aaccgtcgcc atagtagcac agcacttctt
     1081 tttgcggatg aacgaacttg gtcgccatcg agaacgaagt gccgacgccc aaggtgccga
     1141 gcggcccagg gtccatccag ttaccggctt tgcgcggctc caccgccgtc gcggtgatcg
     1201 tcaccacatc gccgccatcg ccgatgtaga ccgtgttatc ggtcaggaat tcattgattt
     1261 cgtaggcgac ccgataaggg ttgatcggcg ttgactccga cttgaacgac ggcaagagtt
     1321 tttcgtaagc cttggcttct gcggcacgca attctttcat ccactcggcg cgctgcgcgt
     1381 tacctttcga gacccgcccg ctggcggctt gctccaccgc ttttagaatc gcgccgcagt
     1441 cacccacgag tccaagcgaa atatcacggt tcttgccgac gttgcggtaa tcgagatcga
     1501 tctgcaccac ggtcgcatcg gcgcgcagcc gccggccgta gcccaagcgg aaatcgaacg
     1561 gacaaccgac gatgagaatc acatcggctt tgtcgaaagc catgcggcgg gtacggttaa
     1621 aaccatgggg atcctccggc gcaaaaatgc cgcggctcga accattcatg taaccgggca
     1681 tgttgagggc gcggcagagc gacaccgcat cgtcatggcc gcgcgaggtc catacttggg
     1741 tgccgaacaa ggcgcaaggt ctttccgagt taacgagaat gtcggcaagc ttttcgatgt
     1801 cggcgggatc accgatcgat ttagtcgacg cgcgataggc gccgggcttg ggaattcgtg
     1861 cgcgctccag gggcactttg gcatcgagca cgtcacgggg aatttctaaa tagctcggtc
     1921 ccggagcacc gttgaccgct tcgcgaaacg ccatcgacac catgtcggcg acccgttcgg
     1981 tgtgcggcac gctggcggcg aatttggtga tcggtgccat gatatcgaca tggggcaaat
     2041 cttgcagcga gcccatctta tgctgcgaca acgcgccctg gccgccgatc atcaccagcg
     2101 gactctcggc gcgaaacgca ttggcgatgc cggtcatggc gttggtcgtg cccggccccg
     2161 ccgtcgtcac cacgcagcca atcttgccgc tctggcgcga ataaccgtcc gccgcatggg
     2221 ctgccacttg ctcatggcgc acgtcgatga ttttaatgcc ctcgtcgata cagccatcgt
     2281 aaatgtcgat gatatgaccg ccgcacagcg tccaaatcac ctcgacgcct tcgttcttga
     2341 gcgccttggc gaccaggtgg ccgcccgaaa tcactggacc ggttgcttct tttttacctt
     2401 cgtttgcggt cgttgccatg gttacctcac ggttggagtt agatttatcg acgcgcacac
     2461 catcactgtc agggaatttg cgctacatga ttagtcgaag gccctgccaa gcgcggtgct
     2521 tacgatatcg aatcccgcag aaagcctcaa gtcgcaggaa ccagaaagcg atcccaagga
     2581 atctaacaca aaatgaatct atactggtgt ggcggagcag gcaggaaagc aatgcttcca
     2641 cttggagggg ctaaatctcc cggcgccctt ccaaagcttt gcctagggta acggcgtcgg
     2701 tgtactcgag atcaccgccc atcggcaaac cgcgggcgat tcgggtgacg gagacgccga
     2761 ggggtttaat cagttttgac aaataaaatg cggtggcttc accgtccatg gtcggattcg
     2821 tggcgacgat gacttctttg acggcgccac cgcgcaaccg ctcgagcaat tctttgatac
     2881 gcagcgcatc cgggccgacg ccatcgagcg gcgagattgt tccatgcagc acatgatact
     2941 ggccatgata ctcttgggac ttttccaccg cgatcatgtc cgccggctct tcgaccacgc
     3001 aaatcttgtc ttcttcacga tccgcgttgc ggcaaacatc gcactgggtg ccatcttcgt
     3061 tctgcgcatg gacttcgctg aaaccgaagc agcggtcgca tagccccatt tcctggcgca
     3121 atcggccgat gctgtcggct agacgaaaaa catcatcccg tttgcccttc aacatatgaa
     3181 acgctaaacg cgccgccgat ttttcgccga cacccggcaa tttggagagt tcttgaatca
     3241 atcgcgccaa aggcgcggga tacatgctca tgagcgggcc tagcttgtcg catcagcgat
     3301 gttcgatact ttcatctcgc tgttgccttg tctgtagttg tcactaatca ttaaaataca
     3361 aactacggca tcagcccagg aatcttgaaa cccccggtga gcttagccat ctccgccgac
     3421 gccatctcgc gcgccttgcg catgccgtcg ttgacggcag cgaggaccag atcttcgagc
     3481 atgcctttat cctcaccgct gatcacggac ggctcgatct ttaccgcggt gagattgaac
     3541 tggccgttta ccgtgacctg gaccatgccg ccaccggcgg caccgtcgac ggtcttatgc
     3601 gccagctcct cctgaacctt ggccatgcgc gcctgcatat cctgcgcttg cttgagcaga
     3661 ttgcccattc ccggaatgct cgccatgctt tgcctcctac tattgccgcg atcgcaaggc
     3721 gatcggccta aggattttct cgccgcaccg aacgcaccga accaccaaaa atgcgcaacg
     3781 cttccttgac catttcacta cggtcctccg cggcgccctc ggcgcgtgcg gcgtcgaaac
     3841 tcgtcggtgg cgcgacctcg cccgccgccg cgacgaactg aattatggca tcgacaccga
     3901 aaaacctctt ggccaggtca cccagcgccg caagattgtc ggcgtcattg agaaacccca
     3961 aatgatgccg ctcggcgaca ctgaccttga gcggtgccgg cggcaacgaa agggccgcac
     4021 tagcgcccag atgcgacgct agaagttttt tttccttacc gacaaaggcg atgaattcgc
     4081 gccatttcga aacatcgtca tccgccgccg ggatgctagc gagcggtgac atggtcaccg
     4141 gcgccttgat taccaccggc gcggccaccg gaacagccct cggtcgctcc gccgcaactg
     4201 gagaccgtac ttctccagat atccgcccct ctaaacgttc gagccggctg agaatttccg
     4261 tgaccggcaa cgcctgcggc agattcgcca agcggatcag cacggcttcg agggcgaatc
     4321 ggggcgtcgc cgaacgcgcc acgtcttcat cgccggcagc gataaattcg aggtaatcga
     4381 gcagcaactc aaccgacaca tgactgctct gccggactaa atcggcaatc tcctgatcgg
     4441 gcagatcgag aagttgcgcc atgtcagcgc cgccgacgcg cgccgcgccc atccggcttt
     4501 cggtcaggcg agcaactagc aagttcctaa aatgttccac cagatcgcgg gacaaccggc
     4561 ttaaatcccg cccctgatgc atcacctcgg cgatcagttc gacgcagcgg cgcgggtcgc
     4621 catcaagcac cgcctgggac aattggtaga ggctttggcg cggcgccagc ccaacaacct
     4681 cttgcaacgt ggttgcgtcg acggtggcct gctgctcgtc ccccaccgcc ggcatggcgc
     4741 tggcgagcac ctgttccaaa agcgactggg catcgcgcat gctgccgtcc gcttcacgcg
     4801 ccagcaacac cagcgcctcg tcggtgatgc gcagcccttc gctttttcca atcgcagcga
     4861 gccgctcgac gatctcgcgc aggctgatgc ggcgaaaatc gtagcgctgg caacgcgaaa
     4921 gaatcgtctc cggtaaacgc tgcggctccg tggtggcaag gataaacttg accgacggcg
     4981 gcggctcttc caaggttttc agtaaggcat tgaatgcatc cttggtaacc tgatgcactt
     5041 cgtcgatgat ataaatcttg aaacggcact tggccggctg gtaacggacg ttttcgatga
     5101 tttggcggat ctcgtcgatg ccacggttcg atgcgccgtc gatctcaagc acgtctatcg
     5161 agctgccgct ggtgatctct tcacattgac tgcattggtt gcacggcgtc gcggtgggcc
     5221 cccgttcgca atttagcgct ttggcaagga tccgtgccgc ggtggtttta ccgacaccgc
     5281 gcaccccggt aaacaggtag gcatgcgcga tgcggttcgc cttgatagca tttttgagtg
     5341 tgcgggtgat cggttcttgg ccggcgacat cgtcgaaatt ctgcggccgc catttacgcg
     5401 ccaaaaccag gtaagacata aatcccttga gaagtcttat actaaacgat gccgactctc
     5461 agcgaactgc tcaggagcga cgatcggcgt atacaaatag attgtcacga ggtgaggata
     5521 cccagtgctg acagccaggt gcccctgtgg cacaggggag agccgttacc gctgcttcct
     5581 tccggacctg acggagttca cggggccgcc ttgcacagga cctaactatc agcactcgac
     5641 atccgcacgc actgtaacac cgccaaaacg atgctgggaa aaacagtacc ccgatcactg
     5701 ggcggtttca agatttactg cttgccatcg agatcttaat cgaaagcggc cgatgagccc
     5761 gctgcgctgc atgtaccatg gcggagaggg agggattcga accctcggta cccttttggg
     5821 gtacacacga tttccagtcg tgcgccttca accgctcggc cacctctccc tatcgaatat
     5881 taaaaactta cgcctctgcc ccgctggttc gactgtcaat gactccaact gtcttcatcg
     5941 agctgttttt ggatcgtttt gatgagctgc tgtggtcaga agttaacaca agctttcaca
     6001 gtagcaaaga cagaatgcga gaaacggaat ttttaacgat cgcgatgaaa acaagcgcct
     6061 attttggcac atactcgcag ctcccgagat cgggtgacct cccataccac tcctggcaat
     6121 agtcgccgtt actcattcgc ggttgatcga gtgccgaacc gggcttgggg caatgaaacc
     6181 ctgggattaa actgccacga tccaccgcag ggctatttgc tgacgggaga ttgcctgacg
     6241 tcgcgcagaa tggatttcca gcccatgtct gcgttccgtt tgtccaagtc attgcggtac
     6301 ctgcaactgt gggaaggttg ggtaggttag caggggcacg cggggtaggc gcgtcttggc
     6361 ttactgaatt ttgcttgcga ctggaggcat cgcgcagaat ggcttccaag cccgggtctg
     6421 ggatataaca gcccacaccg acaagagcca accagagcac ccctattgcc cacagga
//
LOCUS       DASQKS010000618         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1207851, whole genome shotgun sequence.
ACCESSION   DASQKS010000618 DASQKS010000000
VERSION     DASQKS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207851"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1449)
                     /locus_tag="VE170_15925"
     CDS             complement(214..1449)
                     /locus_tag="VE170_15925"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ16980.1"
                     /translation="MTAPPSRLSLFRQRDFCVFWTGSFLSSMGTQFTTVAMAWQIYEL
                     TNSPMQIGLLGLARALPQIVLLLVGGLLADAMDRRKLMMCTQTGLICISTTLALLTYF
                     GKASPHMLYAATMLLAIFTSLEQPSRQSIIPNLVPRDHLAQALALQGTQRYVPVIAGP
                     SLAGVVLAFSGPAACYAVDACSWLAMLSALRMLRTKIPEGGGWKSVSLASLREGWAFV
                     WSHSVIFPLMLLDFSATFFGNARGLFPIFARDILFVGPAGLGLLYAARAVGSLLAAIG
                     MTLVGPVIRGGRWIVIGIGIYGLSTALFAQSQLFWFSVIMLMLTGAGDTVSSILRSTI
                     NQLSTPDELRGRMSSINSIFTSNGPQLGQFESGLVAAWLGARMSALTGGLATLMILVA
                     VTAGYPVLRRYRMPQTSKT"
     gene            1739..2695
                     /locus_tag="VE170_15930"
     CDS             1739..2695
                     /locus_tag="VE170_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HYJ16981.1"
                     /translation="MHKWSLKLMEIAALVIVLGVATAHAQSPGSWTMKAPIPTPRNEV
                     ALAAVGSKIHVIGGGVQGVAGTYHDEYDPATDSWRTRAPLPHGLDHIGAAVLNGKIYT
                     VGGFVGSTHKDGQPHVLEYDPAADSWRTLSPLKVGLGSVGVTALDGKIHAVGGRTPAG
                     QTVATHQVYDPKTNTWSERAPLPKARDHMALVAAEGKIHAVGGRFGASSDKTDMHDIY
                     DPATDSWTSGPPLPTGRSGVAATLYQGMVLVLGGELAPNTFAENEAYDPKTKNWRTLA
                     PMPAGRHGTAAATTGGRLYIVAGSLKPGAGGVTDQLIVFSVP"
     gene            3076..4668
                     /locus_tag="VE170_15935"
     CDS             3076..4668
                     /locus_tag="VE170_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16982.1"
                     /translation="MAKLRKQRQRAARKAQAEPIGAAWLPRLSARAEAILFAFVALIV
                     SALLHFSSDRFADPDGFYHFRHAALYGEGNLFSSAFPWLPFSVMGKYSADIWYGFHIL
                     LIPFTWIGDPVLAMHLAGLVLTWAFLLTIYCACAKLELKSAWIWPFALLVSSAFLLHR
                     VTMPRPQVLSLGLTVLLLEQIVHGRLRGVFVITFAIAWVHLSLFFAPLILLGLFPAIK
                     IFTVRELRWQEFVAGCAGLVAGWLLRPNPLGAASIAYIQVVDLTLANFAHAPLEFGAE
                     LRPLVLTYSSNYLPFILLWLASLLFLLWRAIGKRSQFALSIKTALLLSAILSSVFFLV
                     AILFARRGFDFCSAFGVLVLALAVGEIMPGKKWPRVAVVGLFCVLAVYSVTQRHRVVA
                     LGWDVNQAEPAARWLGANSNAGDVVFNFRWYYFPELFFWNTKNVYTSGMDPIFQYAFD
                     PQLYLSGLKIVAHLPSILCPTGACADPAGVDAFKLFKERYRARYIYLLKEFDANAYFH
                     FLSDSRFALRYEDRGAAVFEAL"
     gene            complement(4677..5645)
                     /locus_tag="VE170_15940"
     CDS             complement(4677..5645)
                     /locus_tag="VE170_15940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ16983.1"
                     /translation="MRYRNSLLLAVMLCLSLFIAARASRAEPIRIAMPSKSLTFLNFY
                     LADRFGLFKNEGLEASFPVGKADVQLTAVITGEMEYIAAAGTSLRGAATGLPVKAMMF
                     ALDKPLFFMMVKPETKRIQDLKGKTVAVTAVAATDALGARAMAKAAGMNADQDLVFLS
                     AGNTANALAAVQAGSAAAAMLSIPFNFKADELGYRSLGNAADFLRTVLSGLATSDARI
                     KTNPAQIKRTIRASLRGMDLARDPANQEKIIPYIMEDFQLDRKTAAASLREIVKHYSK
                     DGTTSEDAVRTDIEFVREQNKLKTAIPTTKVLDYSLLKEVLAELKK"
     gene            complement(5767..>6464)
                     /locus_tag="VE170_15945"
     CDS             complement(5767..>6464)
                     /locus_tag="VE170_15945"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16984.1"
                     /translation="PIILDLKDPTKPEEVGRWWMPGQWTAGGEKPTWEGTAHQCHHPI
                     RYGDRLYISYWHGGFVILDIADMSKPKFISGLNWSPPYPSPIHTTLPIPWKLKGRDMM
                     VVVDEEAGKRAPTPPAFMWMVDITDEKQPVSVSTFNPEGAWDIAPGNQYGAHQPAEQV
                     YDNKMFVTWFSGGLRVVDISNPYSPQEIGYYVPTPGRGQKTVKSNDVYRDANGLVYLI
                     DRLDGLEILESDV"
BASE COUNT         1314 a         1820 c         1909 g         1421 t
ORIGIN      
        1 tttggccgat atttatgaga aagaaaaaag acgcctaatc acaaagtacc aagggtcgag
       61 cgcggcaaca taaacggatt agctgcaaac aacgcaagtc gcaaaaggga attggatggc
      121 cgaacctgat ggaaaagcgc aatactccac ttaggagaac atctttcaac taaccgcagt
      181 gctcatgcca agaatcttgc cgcgccgttg cttctatgtc ttgctagtct gtggcatccg
      241 atagcgccgc agcacgggat atccggccgt taccgccacc agaatcatca gggtcgccag
      301 cccacctgtg agcgccgaca ttcgggcacc aagccaagca gcgacgagac cggactcgaa
      361 ttgccccagc tgcggtccat tgctggtgaa aatgctgttg atcgaagaca tgcggccgcg
      421 caattcgtcg ggcgtgctca actgattgat ggtcgagcgc aggatcgagc tgacggtgtc
      481 gccggcgccg gtcagcatga gcatgatcac ggaaaaccaa aatagctgcg attgagcgaa
      541 caacgctgtg gacagtccgt aaataccgat gccgatgacg atccaacgac cgcctcgtat
      601 taccggacca accagtgtca tgccgatggc ggcgagcagc gagccgacgg cacgagcggc
      661 atagagcaat ccgagtcctg ccggcccgac gaaaagaatg tcacgggcaa agatcggaaa
      721 taatccgcgc gcgttgccaa aaaaggttgc gctgaagtcc agcagcatca gcgggaaaat
      781 cacgctgtga gaccagacga aagcccagcc ttcgcgcaac gacgcgagcg agacactctt
      841 ccagccgccg ccttcgggaa tcttggtgcg aagcatgcgc aatgccgaga gcatcgccag
      901 ccacgagcag gcatcgacgg catagcaggc ggctgggccg gagaaggcga gaacgacacc
      961 ggcgagggac ggtccggcga tcaccggcac gtagcgttgc gtgccttgca gcgccagcgc
     1021 ttgagccaaa tggtcgcgcg gcaccagatt gggaatgatc gactggcgcg acggctgttc
     1081 caacgaggtg aagatcgcca gcagcatggt cgcggcgtaa agcatatgcg gcgacgcttt
     1141 gccaaaataa gttagcagcg ccagcgtggt cgatatgcag atcagcccgg tctgggtgca
     1201 catcatgagc ttgcgccggt ccatggcgtc ggcgagcaga ccgccgacca acagcaaaac
     1261 gatctgcggc aaagcgcggg ctaagccgag caggccgatt tgcatcggtg aattggtcag
     1321 ctcgtagatc tgccaggcca tcgccactgt ggtgaattgc gtgcccatgc tggaaagaaa
     1381 agatccggtc cagaagacgc aaaaatctcg ctggcggaat aacgagagac ggctcggtgg
     1441 tgcagtcatg tggagaatct tcgcagccaa ttttttatct caaagcttgg ccaacgacaa
     1501 accaccgtgc caccgcgctg cgccagcgac ctatgcttag cgcttgacgg gtcggctcga
     1561 ctggacctat tgtccgtacg ctgcctagca gttaacccag gaaggctaac ggcaaccgaa
     1621 gcaagatttg gcagcgggta attagccgca acggacgcaa agaacgcaaa aaaatgctct
     1681 taggaattct gaccgtcagg aaaaattgat accccttcag actgggccgg aggacacgat
     1741 gcataagtgg tcattgaaac tgatggagat cgccgccctc gttatcgtgc ttggcgtggc
     1801 gacggctcac gcgcaatcgc cgggcagctg gacgatgaaa gcgccgattc cgaccccacg
     1861 gaatgaagtg gcgctggcgg ccgtgggcag taaaatacat gtgatcggcg gcggcgtgca
     1921 gggcgtggcg gggacctatc atgatgaata cgatccggcc accgatagct ggcgcacgcg
     1981 cgcgccgctg ccgcacggac tcgatcacat cggcgcagcg gtattgaacg gtaagattta
     2041 caccgtcggc ggctttgttg gttcgacgca taaagacggc caaccccatg tgctcgagta
     2101 cgaccccgcc gcggacagtt ggcgcacact cagcccgctc aaggttggcc tgggctcggt
     2161 gggtgttacc gcgctcgacg gcaagattca cgcggtcggt ggacgcacgc ccgcgggaca
     2221 gacggttgcc acgcaccagg tctacgatcc gaagaccaac acttggagtg agcgcgcgcc
     2281 gctacccaag gcgcgcgatc atatggcgct ggtggccgcc gaaggaaaaa ttcatgcggt
     2341 cggcggacgc ttcggcgcca gcagtgacaa aaccgatatg cacgacatct acgacccggc
     2401 gacggactcg tggacctcag gaccgccgct gccaaccggt cgcagcgggg tcgcggcgac
     2461 gctctaccag ggcatggttc tggtgttggg cggtgagctt gcgcccaata ctttcgccga
     2521 gaatgaggct tacgatccga aaacaaaaaa ctggcggacg ttagctccca tgcccgccgg
     2581 ccggcacggc accgccgccg ccaccacggg cgggcgtctc tacatcgtcg ctggatcgtt
     2641 gaaacccggc gcaggcgggg tcaccgacca actcatcgtc ttcagcgtgc cctgacatga
     2701 tgagctgtga agtgacgaag aggcgagcat cattgaaatc atctctagcg cgcgggtcct
     2761 agcgaacctt gaaaccagta cgcgcggaag cgtgcaagct tcgctagtac tggtaagtga
     2821 ttggaactac actgacccgg taaattgcgc ctccgcttaa ataaggtttg cggtaatcaa
     2881 ccgattgcgc caagctggtc gcaattgccg ccaattctgt gatctctaaa cttgccttag
     2941 cttaggaagc acttctgcgg gcccgctaag tgtcgtgttc gtttaatttg ctcgtcaagc
     3001 catccgtagt tagcgcttac cacccgttcg ctttgattga atgcaaaatc tcggctacgg
     3061 ttggctgcaa ctataatggc taaattgcga aaacagcggc agcgcgcggc gcggaaagcg
     3121 caagcggagc ccatcggcgc cgcttggttg ccgaggctta gtgcgcgcgc tgaagcgatt
     3181 ctttttgcat ttgtcgcgct gatcgtttcg gctctcttgc acttcagttc tgatagattc
     3241 gccgatcccg atgggtttta tcattttcgc cacgccgcgc tttatggcga gggaaattta
     3301 ttttcgtcgg cgtttccgtg gcttcccttt tcggtcatgg gaaaatactc ggcggatatc
     3361 tggtacggct ttcatatcct tttgataccg tttacctgga tcggcgatcc ggtcttggcc
     3421 atgcatttgg ccggattggt tctgacctgg gcatttctcc tgacgatcta ttgcgcttgt
     3481 gcaaagctcg agctcaagtc ggcatggatc tggccattcg cactgctggt atcctcggcg
     3541 tttctgctgc accgcgtcac catgcctcgg ccgcaagtgc tgtcgctcgg actgactgtg
     3601 ctgttactgg agcagatcgt gcacggccgt cttcgcggcg tctttgtcat cacctttgcc
     3661 atcgcctggg tccatttgag tctttttttt gcgccgctga tcttgctcgg tttatttccg
     3721 gcgattaaaa tcttcacggt tagggaactg cgctggcaag aattcgttgc gggctgcgcg
     3781 gggctcgtcg ccggctggct gctacggccc aatccgttgg gcgcggcatc tatcgcttat
     3841 attcaagttg tcgaccttac gcttgcgaac ttcgctcatg cgccgctcga attcggcgcg
     3901 gagctgcgcc cgttggtgct cacttactcc agcaactatt tgccgtttat cctgctgtgg
     3961 ttggcaagtc tgctgtttct cctgtggcgc gccatcggca agcgatccca gttcgccttg
     4021 tcaataaaaa ccgcgctatt gctgagcgcg atactgtcat cggttttctt cctagttgcg
     4081 atcctgtttg cccgccgtgg tttcgatttt tgttcggctt tcggcgtgct ggtgttggca
     4141 ctggcggtcg gcgagatcat gcctggcaaa aaatggcccc gcgtcgccgt cgtcggcttg
     4201 ttctgcgtgc tagcggttta cagcgtgacg cagcgccatc gcgtggtcgc gctcggctgg
     4261 gatgtcaatc aagctgagcc ggcggcgcgc tggctcggtg ccaactccaa cgccggcgat
     4321 gtcgtgttta attttcgctg gtactatttc ccggagctgt ttttttggaa taccaagaat
     4381 gtttacacca gcggcatgga cccgatcttc cagtacgctt ttgatccgca gttgtatttg
     4441 tccggtttga aaatcgtggc gcatctgcca tcgatcctct gtcccaccgg cgcctgtgcc
     4501 gatccggcag gcgtggatgc gtttaagttg ttcaaggagc gctatcgcgc gcgctatatt
     4561 tatttgctca aggagttcga tgctaacgcc tattttcact tcctgagcga ctcgcgattc
     4621 gcgctgcgct atgaggatcg tggcgccgcg gtgttcgaag cgctgtgagc ttcagatcac
     4681 tttttcagct ccgccaacac ttcttttaac aaactgtagt ccaatacttt ggtggtgggt
     4741 atcgcggtct tgagtttgtt ctgctcccgg acgaattcga tatcggtgcg cacggcgtct
     4801 tcgctggtcg tgccgtcttt ggagtagtgc ttgacgatct cgcgcagcga agccgccgcg
     4861 gtcttacggt cgagttgaaa gtcttccatg atgtagggaa taattttttc ctgatttgcc
     4921 ggatcgcggg caaggtccat accgcgcagc gacgcgcgga tggtgcgttt gatctgcgcc
     4981 ggattggtct tgatgcgcgc gtcggaggtc gccagtccgg acaacactgt gcgcaggaag
     5041 tcggcggcgt tgccaaggct gcggtaaccg agttcatccg ctttaaagtt gaaggggatc
     5101 gacagcatcg ccgctgccgc cgaaccggcc tgcactgccg cgagcgcgtt ggcggtgttg
     5161 ccggcgctca aaaaaaccaa atcttgatct gcattcatgc cggcggcctt cgccatggcg
     5221 cgggcgccga gggcgtcggt ggcggccacg gcggtgacgg cgacggtttt tcctttgaga
     5281 tcctgaatgc gtttggtttc gggcttgacc atcatgaaga acagcggttt gtcgagagcg
     5341 aacatcattg ctttcaccgg cagaccggtg gccgcgccgc gcagcgaagt gccggcggcg
     5401 gcgatgtatt ccatctcgcc ggtgatcacc gcggtgagct gcacgtcggc tttgccgacc
     5461 ggaaaactcg cttccagtcc ttcgtttttg aacaggccga atcggtcggc caggtagaaa
     5521 ttcaaaaacg tcaggctctt ggacggcatg gcgatgcgta tcggttcggc gcgagaggcg
     5581 cgcgcggcaa taaacaggct aaggcaaagc atgactgcta ataatagact attgcggtat
     5641 cgcatgatgc tttccgtttc ggattttctc gcaacctcgc caatgtcaca gtccgatgcc
     5701 cgccatcctg agcgcaggcg acggaaccaa taaagctttc tcccttcgat cgaaatcaaa
     5761 tcaaattcat acgtcgcttt cgagtatctc caagccatcc aagcggtcga tcaagtagac
     5821 caggccgttg gcgtcgcgat agacgtcatt gctcttgacg gttttctggc cgcgtccggg
     5881 agtgggcaca tagtagccga tttcttgtgg gctataggga tttgaaatat ccacgacgcg
     5941 caggcctccg ctgaaccagg tgacgaacat tttattgtca tagacttgct cggccggttg
     6001 gtgtgcgccg tattggttgc caggcgcaat gtcccacgca ccctcgggat tgaaggtcga
     6061 caccgatacc ggctgttttt catccgtgat atcgaccatc cacatgaacg ccggcggcgt
     6121 cggcgcgcgt ttgccggcct cctcatcgac caccaccatc atgtcgcggc ccttgagctt
     6181 ccatggaatc ggcaacgtcg tgtggatcgg cgaaggatag ggcggcgacc agttgaggcc
     6241 gctgataaat ttcggtttgc tcatgtcggc gatatcgaga atcacgaaac cgccgtgcca
     6301 atagctgatg tagaggcggt cgccgtagcg gatcggatga tgacactgat gtgccgtgcc
     6361 ttcccaagtc ggtttctcgc cgccggcggt ccattgacct ggcatccacc agcggcccac
     6421 ttcctccggt ttggtcggat ctttgagatc caaaatgatc ggaa
//
LOCUS       DASQKS010000619         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4959029, whole genome shotgun sequence.
ACCESSION   DASQKS010000619 DASQKS010000000
VERSION     DASQKS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4959029"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="aroB"
                     /locus_tag="VE170_15950"
     CDS             <1..1047
                     /gene="aroB"
                     /locus_tag="VE170_15950"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HYJ16985.1"
                     /translation="RSYPIHIGAGNLPRIGQFLQQAGLSGKVAVVTNPTVAPLYLDSV
                     GNALKESGFAVTPVVIPDGEEYKTVESLQSIYDCLIAQRFERKSVVLALGGGVVGDMG
                     GFAAATYLRGVPYVQVPTTLLAQVDSSVGGKTGVNHADGKNLIGAFYQPRLVLIDVSV
                     LRTLPRRELCSGLAEVIKYGVIQDPEFFRLLEHKIAALSRLDEELLIQTIATSCAIKA
                     RVVEVDEREDDYRAVLNFGHTIGHALEAVTGYTQFLHGEAVAVGMAKAALLSARHGFC
                     DAQSLQRIVELIENAGLPSRIPVDISLTGLVQAMEVDKKAAGGAIKFVMCEGIGKTRF
                     HWLAPGEILRGLGA"
     gene            1087..1527
                     /gene="aroQ"
                     /locus_tag="VE170_15955"
     CDS             1087..1527
                     /gene="aroQ"
                     /locus_tag="VE170_15955"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HYJ16986.1"
                     /translation="MAKPPKILILNGPNLNLLGTRQPDIYGRLTLAQIEKRVRALAKE
                     LAVEIDFRQSNSEGELVTWIQQAAGKFAAIVINPAAYTHTSLAMRDAISAVGVPTIEI
                     HISNIHKREQFRHHSFIAEVVVGQIAGFGLDSYLLGLRAAVARL"
     gene            complement(1540..2316)
                     /locus_tag="VE170_15960"
     CDS             complement(1540..2316)
                     /locus_tag="VE170_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16987.1"
                     /translation="MIMQMTPVLALFVSLYWIDQCEAQYVLILNNGRQITVQSYREDA
                     GMIKFNGFGGEIGIDKGQIKAIRKSVEGAANGYTIPGPTREYSNTTESNPPPPPSGER
                     SEPAPPPEDEQAKDEKEYQQKLLDVNERLKAARDDYSQLIRGTTSAEPSLLTTEAQLK
                     AREDDIISRALDAQYKPSAPATTRLVTPSPFTTLPPATVETQPPPEASPRYGTALPQY
                     TPRQKELSALRSQILDLETQRERLIEDMNKKNFSSGKLLE"
     gene            2461..3717
                     /locus_tag="VE170_15965"
     CDS             2461..3717
                     /locus_tag="VE170_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ16988.1"
                     /translation="MWLAFCLTATVTLGWSAEPKIQILSPKDGSQIAEDQRTILISGK
                     VASDSSRSANVDIFLILDTSGSTVNSAGAEFASQAELPDKYVFGGVPRLQPQISISRR
                     GMGMGAPTFEPTRINLRHSILGAEVVASRRLLTQLNPQTTRVGVITFAKDGRLIQPLT
                     NDFEQVRRVLDEVYWAGPNGGTNMVEGIRVGISELLGLGQSAKRQDAIKVQFFLTDGL
                     PSLPTGDGRSFAETDVQLTVNAARLAGRAGIKIHVFGLGDEVVNYPWAVQGIAKESGG
                     TYTPLSRPADVLAIMDQISVVGVDFVQIVNQTSGQKAANLRLAADGFFAAALPVVEGK
                     NQIDVLARSSDGSNSRASISVFYQAGNQKSLELEIFLEREKKLKLEVERLGRSSEEIQ
                     RDVDRQRQEGLSRPQQLPPSTEGPPR"
     gene            complement(3748..>4001)
                     /locus_tag="VE170_15970"
     CDS             complement(3748..>4001)
                     /locus_tag="VE170_15970"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ16989.1"
                     /translation="TESRGTTVTLTPKKEIFVNQRKVSEESLEKALQEALNNNANKTV
                     ILRGDKDVLLGETVKVMSIIKRAGATEIAIAAETEGKGR"
BASE COUNT          835 a         1041 c         1143 g          982 t
ORIGIN      
        1 cgatcttatc ccatccatat cggtgccggc aatttgccgc gtatcggcca atttctccaa
       61 caagctgggc tgagcggcaa agtcgcggtg gtgaccaatc caaccgtggc cccgctctat
      121 ctcgatagcg tcggcaatgc cctaaaagaa tcgggattcg ctgttacccc agtggttatt
      181 cccgacggcg aggaatacaa aaccgttgaa tcgctgcaat ccatttacga ctgtctgatc
      241 gcccaacgct tcgagcgcaa gtccgttgtt ctggcgctgg gcgggggtgt ggtcggcgac
      301 atgggcggtt tcgccgcggc aacctattta cgcggcgtcc cctatgtcca ggtgccgacg
      361 acattgttgg cccaggtaga ctccagcgtg ggcggcaaga ccggggtcaa ccacgccgac
      421 ggtaagaacc tcataggcgc tttttatcaa ccgagattgg tgttgatcga tgtctcggtg
      481 ttgcgcacat tgccgcgacg ggaactctgc tccggtctgg cggaagtgat taaatacggt
      541 gttattcaag accctgagtt ttttcgttta cttgaacata aaattgccgc gttatcgcga
      601 ctcgatgaag aattactgat ccagacgatc gcgacttctt gcgccatcaa ggctcgggtt
      661 gtcgaagtcg acgagcgcga agatgattat cgtgccgtgc tcaacttcgg tcacacgatc
      721 ggccacgcct tggaagcggt cacgggttat acccagtttc tccacggcga ggcggtggca
      781 gtcggaatgg caaaagccgc gctactatcg gcgcgccacg gcttttgcga tgcgcagagt
      841 ttacagcgca tcgttgagtt gatagaaaac gccggactgc caagcaggat ccctgtcgat
      901 atttcattga ctggcttagt tcaagcaatg gaagtcgaca agaaggccgc cggcggtgca
      961 attaagtttg tgatgtgcga aggcatcggc aaaactcgat ttcactggtt ggcgccgggc
     1021 gaaattttgc gcgggctcgg ggcttaattc cagttatcct cggctagcga aagcgggaaa
     1081 gcaacaatgg cgaagccacc gaaaattctg attctcaacg gacctaattt aaatcttttg
     1141 ggaacccgac agccggatat ctacggcaga ttaacgttgg cgcagatcga aaaacgagtg
     1201 cgcgcattgg ccaaggagct ggcggtggag atcgattttc gtcaatccaa tagcgaaggg
     1261 gaactagtaa cttggattca gcaagcggcg gggaagtttg ccgccatcgt gatcaatcca
     1321 gcggcctaca cgcacaccag cctagcgatg cgcgatgcga tttccgccgt gggtgtgccg
     1381 accatcgaga ttcacatatc gaatattcat aagcgcgagc aatttcgcca ccactcgttt
     1441 atcgccgagg tggtggtggg acagatcgcg gggttcggat tggatagtta tctgttggga
     1501 ctgcgtgcgg cggtggcgcg gctttaggct gaatacgttc tattctagta actttccgct
     1561 gctaaaattc tttttattca tatcttcaat cagtctttct ctttgcgtct ccagatctag
     1621 gatctggctg cgcagcgcgc tgagctcttt ttgccgcggc gtatattgcg gcagggcggt
     1681 gccgtagcgc ggcgaagcct cgggcggcgg ttgagtctcg accgtcgctg gtggcagggt
     1741 agtgaaggga gacggtgtca ccaaccgggt ggttgcgggg gcgctgggtt tgtactgggc
     1801 gtcgagtgct cgtgaaatga tgtcgtcttc gcgcgccttt agctgtgctt cagtggttag
     1861 caggctcggt tccgcactgg ttgtgccgcg gatcagctgc gaatagtcat cccttgctgc
     1921 tttcagccgt tcattcacat cgagtaattt ttgttgatac tctttctcgt ctttagcctg
     1981 ttcgtcttcc ggcggcggtg cgggttcgga tcgttcaccg gatggtggag gcggtggatt
     2041 cgactccgtt gtattagagt actcgcgggt cggtccgggt atcgtatatc cattggctgc
     2101 gccctcgaca gatttgcgaa tcgccttgat ctgaccttta tcaatgccga tttcgccgcc
     2161 aaagccgtta aatttaatca ttcccgcgtc ttcacggtaa ctttgcacgg taatttgccg
     2221 gccgttattc aagatcaaca catattgcgc ttcgcactgg tcgatccagt agagcgaaac
     2281 aaatagtgcc agtacaggcg tcatctgcat gatcatgccg ctgattatat catggcagcc
     2341 cagccagggg tacactttcg tggcgccaca ggtgacgttt acagggtacc tggttcgtgt
     2401 tagcgtttag tccttggggg aaccgatggc atgagggcaa ataatcgccg cggggtaatt
     2461 ttgtggttag ctttctgcct cactgccacg gtgacgctgg gctggtctgc cgagcctaag
     2521 atccaaatcc tttctcccaa agacgggtcg caaatcgccg aagatcaaag aacgattctt
     2581 atcagcggca aagtcgccag cgacagttca cgctcagcta atgtcgatat tttcttaatt
     2641 ctcgatacct ccggtagtac ggtcaactcc gccggtgccg aatttgccag tcaagccgag
     2701 ctcccggata agtatgtctt tggcggcgtg ccgagattgc aaccgcagat atcaatttcg
     2761 cggcgcggca tgggaatggg cgcgcccacc ttcgagccaa cgcggatcaa tttacgtcat
     2821 tcaattcttg gcgccgaggt cgtcgcttcg cgccgtttgc tgacccagtt gaacccgcag
     2881 actacccggg ttggagtcat cacctttgca aaggatgggc gtttgatcca gccgctcacc
     2941 aatgatttcg aacaagtgcg gcgagtgctc gatgaagtct actgggccgg gcccaacggc
     3001 ggtaccaaca tggtggaggg cattcgcgtg ggaattagcg aactcttggg tctaggccag
     3061 agcgctaaac gccaagacgc gattaaagtg cagttcttct tgacggatgg tttgccgtca
     3121 ttgccaacgg gcgacggcag aagttttgcc gaaaccgatg ttcagctgac tgtcaacgcg
     3181 gcaagattgg ccggccgcgc cggaataaaa attcacgtct tcggcctcgg cgacgaagtc
     3241 gtcaattatc cttgggccgt tcaggggatc gccaaagaaa gtggcggtac ctatacgccg
     3301 ctatctcgcc cagccgatgt cttggcgatc atggatcaaa tctcagttgt cggtgtcgat
     3361 ttcgttcaga tcgtcaacca aacctccggg cagaaggcag cgaatttacg tctggccgcg
     3421 gacggctttt tcgcggctgc tcttccggtg gtcgaaggca agaatcagat cgatgtcctg
     3481 gcgcgctcca gcgacggctc caatagccgc gcatcaatct cggtctttta tcaggcggga
     3541 aaccagaagt cgttggagct ggaaattttt ctcgaaagag aaaagaaact caagttagaa
     3601 gttgaaagat tgggcaggag ttccgaagaa attcagcgcg acgtcgaccg ccagcggcaa
     3661 gaaggattga gccggccgca acagttgccc ccgtccacgg aaggtccgcc gcgctgattc
     3721 ccgtcattca gcaaaattaa aggttcgtta gcgtccttta ccttcggttt cggcggcaat
     3781 ggcgatctcg gtggcgccgg cgcgcttgat gatcgacatc actttcacgg tctcgccaag
     3841 caacacatct ttatcgccgc ggagaatgac agtcttgttg gcattattgt taagcgcctc
     3901 ttgaagggcc ttttctaagc tttcctcact cacttttctt tgattgacaa agatttcctt
     3961 ctttggtgtc agcgtcacgg tggtgccgcg gctttcggtt t
//
LOCUS       DASQKS010000620         9139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2008408, whole genome shotgun sequence.
ACCESSION   DASQKS010000620 DASQKS010000000
VERSION     DASQKS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008408"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VE170_15975"
     CDS             complement(<1..729)
                     /locus_tag="VE170_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYJ16990.1"
                     /translation="MKLRRWAPWFWAVAVAVVVLHNGYLWWQQRLAPDTDILALLPAL
                     GRDPVVQRALPQMVEAAEQRVIVLIGATDWDDARRAAAVYRKSIAPFPHLLFAAAAIT
                     ERTRTDWLETFKNHRLTLLTSEDDTALRTQAPSHWVDRALAKLYSPFAGPQPLAWQED
                     PFGLFNNWLLARAEETPVRPREGELSVSAGELHYVVLPLTLRVPAFALAGQQAVMPVL
                     EQARQAVRQTTKRIEVIQAGVILHA"
     gene            complement(965..1567)
                     /locus_tag="VE170_15980"
     CDS             complement(965..1567)
                     /locus_tag="VE170_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HYJ16991.1"
                     /translation="MNRKILAICLSLMAAPLLAAAPVAEIQAILAQPKVLCGRFDQIK
                     QLAGLKNPLRSNGRFCVVADKGVLWRSLQPFANTLRLTRDEIVQWQGERLAMRLNAKQ
                     EPTVRIINSLLFSVLAGDLGQLEKHFDVDGSVSEGAWSVKLKARDAGVAKVIGAIALD
                     GAAYVKNIAIDEANGDRTRIVFSALQTGAQAMTSEEAALF"
     gene            complement(1564..1992)
                     /locus_tag="VE170_15985"
     CDS             complement(1564..1992)
                     /locus_tag="VE170_15985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016351786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HYJ16992.1"
                     /translation="MSRIPSRWFAETETQVQFNHLDPMEVVWHGHYVNYLEIVRSALL
                     TSIDYNYPQMKASGYVWPVIDLHIRYVAPAKFAERLKLRAEIVEWENRLEIAYLLTDA
                     ATGRRVSRASTTQVAVELATGEMCFVSPPVLFEKLGLTPP"
     gene            complement(2142..3698)
                     /locus_tag="VE170_15990"
     CDS             complement(2142..3698)
                     /locus_tag="VE170_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HYJ16993.1"
                     /translation="MQPARVTNPSPTVCFDTGWRSIEEIVAVAEKAAAASLSAAPQFR
                     AAIARGADFLDRRLAEDGVIYGVTTGFGDSCTVAIDPELIAALPRHLYIFHGCGLGAH
                     FTPSETRAAIATRLTSLCKGFSGVSVGLLEQLVNLLKHDIVPAIPCEGSVGASGDLTP
                     LSYLAAVLCGEGEVLRGGAVVPAASILSEAGITPLRLRPKEALAIMNGTAVMTALACL
                     NYIRAEYLTRLTTRISALASFALDGNAEHFDATLFSVKPHAGQQSVAGWLRQDLPVQV
                     SAANDKRLQDRYSIRCAPHVIGVLADALPWLRSYIENELNSANDNPIVDAVGERILHG
                     GHFYGGHIAFAMDSMKNAVANLADLLDRQMALLVDSRYSNGLPANLSGVTGPRAAINH
                     GLKALQISVSAWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDCTRVLELTEQV
                     AAGLLITVRQAAWLRCRANPALAPGPSLQAMIDVLSGDIAVIEEDRRLEPDLRLLLER
                     MRSRAWKIYQ"
     gene            complement(3685..4656)
                     /locus_tag="VE170_15995"
     CDS             complement(3685..4656)
                     /locus_tag="VE170_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYJ16994.1"
                     /translation="MPAAKAAGAGRHWAQIDEVSFVAGMRLLFWLGRVLGRWPFRVVL
                     YPVMLWYAIAKPWARTASQDYLRRMAAFDAGSGSATGVMGVLYHFASFGECILDKLLL
                     WCGLFDTGAVEIHGDEPVAAQVVGKRGGLIICCHLGNLELCRVMSKRQPGLKLTVLLH
                     TKHATRFNQLLAQVNPDSQLNIIQVTELTPAVAILLQTKIDQGEFIAIAGDRIPVAPQ
                     PRVARARFLGAWAPFPVGPYVMASLFRCPVYLLFSLSTDHGWEIHIELFRESIRLPRK
                     DRSAALDALARAYAARLEAYCRREPLQWFNFYDFWQVAPLKDEHAAR"
     gene            complement(4656..5399)
                     /locus_tag="VE170_16000"
     CDS             complement(4656..5399)
                     /locus_tag="VE170_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ16995.1"
                     /translation="MHRPCAVIPVYNHPAAVTAVVGGMLAQPLDCIVVDDGSTPACAA
                     VLDRLAAATPSRVTLLRHPTNRGKGAAVLTAVRYAAQAGFTHALQIDADGQHRVEDSA
                     SFIALSAAHPNALVIGSPRYDDSVPGLRLWARHLTHIWVSINTLSRRIEDSMCGFRVY
                     PLAPLLELDRQRKLGARMNFDIEVLVRLHWAGVEIVNLPTAVSYPKDGISHFRGGLDN
                     LLISRLHATLFFGMLRRLPTLLARKRGVL"
     gene            complement(5392..7182)
                     /locus_tag="VE170_16005"
     CDS             complement(5392..7182)
                     /locus_tag="VE170_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ16996.1"
                     /translation="MAHPLNLLSTLNASRSADQIIGWRGGVAVSWSEFHARVTDWSVL
                     LGESPGDAFALWHHDAIEFAAALFGAWLARKTVLLPGDNLPGTCAGLRGNVAGFLGEF
                     AAEWQPKAAPAGNRHHQPDRFAAIDGDFTALVLYTSGSTGAAQPVAKNLDQLVAEVAH
                     LEKQFGALLGAADVISTVSHQHIYGLLFNVLWPLTAGRRMQASTVSWFEDLSANLAAR
                     DALLVSSPAHLSRIPENPRWAEAAERLRAVFSSGGPLGFEVAQECRRLLGRVPIEVYG
                     SSETGGIAWRQQESRQHQAWSALPGVEWRVDAIDAIDALDSIDAMGAIRPMDGTEEVL
                     AVRSKHLPSADWFRTADRAQTTGDGRFILGGRVDRIAKIEGKRISLSAIEKLLTASPL
                     VDLARVIAVDGRRQRVAAFVVPSARGQCELHALGRRGFTRMLRRLLDRFIEPVGMPRL
                     WRFFKALPVNAQGKTSHADLLALLETPQMLLTEPRSRLLERDAERALFELVAPRDLIY
                     FDGHFNGQLILAGVVQIDWAIAFGRRCFALPPQFRAIQMLKFQRLILPDTPFRLELVY
                     QPASATLSFKISTALGTHASGRLLFGAADA"
     gene            complement(7157..7723)
                     /locus_tag="VE170_16010"
     CDS             complement(7157..7723)
                     /locus_tag="VE170_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ16997.1"
                     /translation="MRGALRILAILITLVFPLALWLGEGRVAPAWLAALLLLGGLARL
                     PATAPTGATRYWTGATILLALSTFWSGALLPLRFYPVLVSGAFLTVFAYSLVVPPSTI
                     ERLARLHEPGLSPAAVAYTRRVTQVWCVFFALNGAIALYTALFGSMAQWSFYNGFLAY
                     LLMGIIFAGEYCARYYFKRRHGSSAQSA"
     gene            complement(7730..7993)
                     /locus_tag="VE170_16015"
     CDS             complement(7730..7993)
                     /locus_tag="VE170_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYJ16998.1"
                     /translation="MTSALPVRKEEIYPWLVNMLHEMFELDAAKITLDAHLYNDLDID
                     SIDAVDLAVRLKQLTGKRLQPEVFKKVRTVGDVVEALAELIEQ"
     gene            complement(7990..8265)
                     /locus_tag="VE170_16020"
     CDS             complement(7990..8265)
                     /locus_tag="VE170_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYJ16999.1"
                     /translation="MRVTEEDIKQLIIDVLQLEDIAPADIDAQAPLFVEGLGLDSIDA
                     LELGVALQKRYGIILSAKAEENRRHFASVRALAEMIETVRKNEEQSQ"
     gene            complement(8252..9022)
                     /locus_tag="VE170_16025"
     CDS             complement(8252..9022)
                     /locus_tag="VE170_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ17000.1"
                     /translation="MFKRIEWCWRVFGVALSFIAIGIGGLVIFPLLRLIIWQRQRRQA
                     VARDLIRLAFLGIVRSMCAFGVFQYQANGLARLERQGLLILANHPTLIDIVFLMAFVP
                     RADCIVKQGLWRNPFTRATVKAAGYICNRGDSVRLVEDCVASVRGGGNLIVFPEGTRT
                     PPDGSISLKRGAANIAVRAPCDITPVLVRCAPPMLIKSEPWWRLPAQTSQFHFDVMED
                     IAIGPFVEGAANHVLAARRMTAHLLQIFAQEGESRASN"
BASE COUNT         2023 a         2783 c         2701 g         1632 t
ORIGIN      
        1 ggcgtggaga ataacgccgg cttgaatgac ctcaatccgt ttagtggttt gccggacagc
       61 ttggcgcgcc tgctcgagca ccggcataac cgcttgctgc ccggcgagag cgaacgccgg
      121 cacccgcagg gtcagtggta agacgacata gtgaagctcg ccggcgctga cggataattc
      181 gccctcacgc ggccgcaccg gagtttcttc ggcgcgcgct aatagccaat tattgaacaa
      241 tccaaaaggg tcctcctgcc acgccagcgg ctgcggcccg gcgaagggac tgtaaagttt
      301 ggcgagcgcg cggtcgaccc aatgagacgg tgcttgagtg cgcagtgccg tatcatcctc
      361 agacgtcaac aacgtcaacc gatggttttt aaaagtctcc agccagtcgg ttcgagtgcg
      421 ctcggtaatc gctgcggcgg caaaaagcaa atgcgggaac ggtgcgatag acttgcgata
      481 gacggcggcg gcccgccgcg cgtcgtccca gtcggtcgcg ccaatcagca cgatgacgcg
      541 ctgttcggcc gcctcgacca tttgcggcaa cgcccgctgc accaccgggt cgcgccccag
      601 ggccggcaac agcgcgagaa tatcggtgtc aggagccagc cgctgctgcc accaaagata
      661 gccgttatga agcacgacca cggccaccgc cacggcccaa aaccaaggag cccatcggcg
      721 cagtttcaag ccgcattcct caggtgaaat cgccgtggcc ttggcatttc ttcggtcgcc
      781 aaggcaatcg agtgttttgc agagcaagca ttcatcggaa tatctcggtg acgcttcgcg
      841 ccgccatagc gccgggctgc caaagaaaaa ttaaaaactc tgcaacagcg cgctggatgc
      901 tctgcgcgag tgcaaaatcg tgcagcgctc agcaaccggg aaggctgaac tgcgccatcc
      961 gaagttaaaa tagcgccgcc tcttcgctgg tcatggcctg cgcgccggtt tgtagcgccg
     1021 agaagacaat gcgcgtgcga tcaccgttgg cttcgtcgat ggcgatgttc ttgacatagg
     1081 cggcgccgtc cagggcgatg gcgccgatga cttttgccac accggcatcg cgcgctttaa
     1141 gcttgacgct ccaggcgccc tcgctaacgc tgccgtcgac atcaaagtgc ttttccagct
     1201 ggccaagatc cccggccagt acggaaaata acaggctgtt gatgatccgc accgttggtt
     1261 cttgcttggc attgaggcgc atggccagtc gttcgccttg ccactgaacg atctcgtcgc
     1321 gggtcagccg caaggtgttg gcaaagggtt gcaaactgcg ccacaggacg cctttatctg
     1381 cgaccacgca aaagcggccg ttggaacgta acggattttt caaaccggct aattgcttaa
     1441 tctggtcgaa gcggccgcac agaacttttg gctgagccaa gatcgcttga atctctgcca
     1501 ccggcgcggc cgccaggagc ggcgcggcca tcaggctgag acagattgcc agaatttttc
     1561 tgttcatggc ggcgtgagac cgagtttttc aaacagcact ggcggcgaga caaaacacat
     1621 ttcaccagta gcgagttcca ccgccacttg ggtggtgctg gcccggctga ctctccggcc
     1681 ggtggccgcg tctgttagca ggtaagcgat ctccaggcgg ttctcccatt cgacgatctc
     1741 ggcgcgcagc ttgagccgct cggcaaattt ggctggggcg acgtaccgga tatgcagatc
     1801 gatcaccggc cagacataac ccgacgcttt catctgcgga taattgtaat cgatcgaggt
     1861 aagcaaggcg ctgcggacga tttcgagata gttgacgtaa tggccgtgcc aaacgacctc
     1921 catgggatcg agatggttga actgcacctg ggtctcggtt tcggcgaacc agcgactggg
     1981 aattctactc atacgttctt caggctttgt ctggattgag aaaaagggat ttcaccatga
     2041 aggacgcgaa gagcgcgaag ttcggagctt tggtcatccg aaaccttcgt gtccttcgtg
     2101 cccttgtgat gaaaataaat tcgcattaac ccagcaataa cctactgata aattttccat
     2161 gcccggctgc gcatccgttc gagcagcagc cgcaggtctg gttccaagcg gcggtcctcc
     2221 tcgatcacag cgatgtcgcc gctgagcacg tcgatcatgg cttgaagcga cggaccgggc
     2281 gccaaagctg gattcgcacg acagcgcaac caagcagctt ggcgcaccgt gatcaacagg
     2341 ccggcggcca cctgttcggt caattccaat acccgcgtgc agtcgcgcgc cgcgatcgtg
     2401 cccatgctga ccttgtcttg gttgtgacat tcggtggaac gggaaaacac cgacgccggc
     2461 atcgtcagct tgagcgcttc cgcggtccac gccgagacgc tgatctgcaa cgccttgaga
     2521 ccgtggttga tcgccgcacg cggcccggtg acgccggaaa gattggcggg caagccgttg
     2581 ctatagcggc tgtctactaa cagggccatt tgccggtcga gtaaatcggc caagttggcc
     2641 acggcgtttt tcatgctgtc catggcgaag gcgatgtggc cgccgtaaaa atgaccgcca
     2701 tgcaatatcc gttcgcctac cgcatcgacg atcggattgt cattggcgct gttcagttcg
     2761 ttttcaatat aagagcgcag ccacggcaag gcgtcggcga gcacgccgat aacatggggc
     2821 gcgcagcgga tcgaatagcg atcctgcaac cgtttgtcgt tggcagcgga tacttgaacc
     2881 ggtaaatctt ggcgcagcca gccggccacg gattgctggc cggcatgggg tttgaccgaa
     2941 aataacgtcg catcgaaatg ctcggcgtta ccgtccaggg cgaaggacgc cagcgcactg
     3001 atgcgggtcg tcagccgggt gagatattca gcgcgaatat aattcaagca ggccagcgcg
     3061 gtcatcaccg ccgtgccatt catgatcgcg agcgcttctt tcgggcgcaa ccgcagcggc
     3121 gtaatgccgg cctcggataa tattgaagcc gcaggaacga cagcgccgcc gcgcaacact
     3181 tcgccttcgc cgcataagac cgcggcgaga taagacaagg gcgtcaaatc gccgctggcg
     3241 cccactgaac cttcgcacgg gatcgccggc acaatatcgt gtttgagcaa attaaccagc
     3301 tgctccaaca agccgacgct aacgccggaa aaccccttgc acagcgaggt caaacgagtg
     3361 gcgatcgccg cgcgggtttc gcttggcgtg aagtgggcgc cgagaccgca accgtgaaag
     3421 atgtaaagat ggcgcggcag cgccgcaatc agctccggat cgatggcgac ggtgcaggaa
     3481 tcgccgaagc cggtggtgac tccgtatatg acgccgtctt cagcgagccg gcggtcgaga
     3541 aagtctgcgc cgcgggcaat cgcggcgcga aactgcggcg ccgcagataa actcgccgcc
     3601 gccgcttttt cggcgacggc gacgatctct tcgatactgc gccaaccggt atcgaagcat
     3661 actgtgggag agggattcgt aactctagcg ggctgcatgt tcgtctttca gtggtgccac
     3721 ttgccaaaag tcataaaaat taaaccattg caacggttct cgccggcagt aagcctcaag
     3781 ccgcgccgcg taagcgcgcg ccagggcatc cagtgcagcc gagcgatctt tgcgtggcaa
     3841 gcggatcgac tcgcggaaga gctcgatatg aatttcccag ccgtggtcag tgctcagcga
     3901 aaacagcaag tacaccgggc agcgaaacag gctcgccatc acataagggc ccacgggaaa
     3961 tggcgcccag gcgcccaaga agcgcgcccg cgcgactcgt ggttggggcg ccaccggaat
     4021 gcggtcaccg gcgatggcga taaattcgcc ttggtcgatt ttcgtttgca acagtatcgc
     4081 cactgccggt gtcagctcgg tcacttgtat gatgttcagt tggctatcgg gattgacctg
     4141 agccaacaat tgattgaagc gcgtggcgtg tttggtatgc agcagcactg tcaatttgag
     4201 accgggttga cgcttggaca tgacccggca gagttctaga ttgccgagat ggcagcaaat
     4261 tatcagtccg ccccgtttgc cgacgacttg cgcggcgacc ggctcgtcgc cgtgaatctc
     4321 aaccgcaccg gtgtcgaaca aaccgcacca cagaagtaat ttgtcgagaa tgcattcgcc
     4381 gaaggaagcg aaatggtaca gcacgcccat aacgccggtt gcgctgccgc tgccagcgtc
     4441 aaacgcggcc atacgccgaa gataatcttg cgatgccgtc cgcgcccacg gtttggcgat
     4501 ggcataccaa agcatcaccg gatagagcac gacgcgaaac ggccaacggc cgagcacccg
     4561 gccgagccaa aacagcaaac gcatgccggc gacgaaactc acctcatcaa tttgcgccca
     4621 gtgccggccg gcgcccgctg ccttcgccgc cggcatcaca gcacgcccct tttgcgcgcg
     4681 agtaaagttg gcagacgtcg cagcatgccg aaaaatagcg tcgcgtgtaa acgcgagatc
     4741 agtaaattgt ccaagccgcc gcgaaaatgc gatatcccgt ccttgggata gctcaccgcg
     4801 gtgggtagat taacgatctc gacgccggcc cagtggagcc ggacgagcac ttcgatgtcg
     4861 aagttcatgc gcgcgccgag tttgcgctgg cggtcgagtt caagcagcgg cgccaacggg
     4921 tagacacgaa agccgcacat ggaatcttcg atgcgccgcg acaaagtgtt gatcgacacc
     4981 caaatatggg tcagatggcg tgcccacagg cgcagcccag gcacgctgtc atcgtagcgt
     5041 ggcgaaccaa tcaccaacgc attcggatgg gctgccgaca gggcgataaa gctagcgctg
     5101 tcttcaaccc ggtgctggcc gtcggcgtcg atctggagcg cgtgggtaaa cccagcctga
     5161 gccgcataac ggacagcggt gagcaccgcg gcgcctttgc cacgattggt cggatggcgc
     5221 agcaaggtca cgcgactcgg cgtcgcagca gccaagcgat caagcaccgc cgcgcacgcc
     5281 ggcgtactgc cgtcgtcgac gacaatacaa tccagcggct gcgcgagcat gccgccgacc
     5341 accgcggtca ccgccgccgg gtgattgtaa actggaatca ccgcgcaggg tctatgcatc
     5401 ggcggcaccg aacagcaggc ggccgctggc atgggtgccg agcgccgtgc tgatcttgaa
     5461 cgacaacgtg gcgctcgccg gttggtacac cagttcgagt ctgaacggcg tatccggcag
     5521 gatcaggcgc tggaatttca gcatctgaat ggcgcgaaac tgtggcggca aagcgaaaca
     5581 gcgccggccg aaagcgatcg cccaatcgat ctgcaccacc ccggcgagaa tcagctgacc
     5641 gttaaaatga ccgtcaaaat atatcagatc acggggagcg accaactcaa agagcgcgcg
     5701 ctcggcatcg cgctcgagca gccgtgagcg cggctcggtc aaaagcatct gcggcgtttc
     5761 aagcagagca agcagatcgg cgtggctggt cttgccctga gcattgaccg gcagtgcctt
     5821 gaaaaaacgc cacagacgag gcatacccac cggttcgatg aaccgatcga gcaggcgacg
     5881 cagcatgcgc gtaaagccgc gccgtccgag cgcgtgaagt tcacactggc cgcgcgccga
     5941 cggcacgacg aaagcggcca cccgttggcg ccgcccatcg acagcgataa cacgggcaag
     6001 atcaaccagc ggcgaagccg tcagcaattt ttcaatggcg ctcagcgaaa tccgtttgcc
     6061 ctcgatctta gcgattcgat cgacccggcc gccgagaata aacctaccgt cccccgtcgt
     6121 ttgcgcccgg tccgcggtgc gaaaccaatc tgcactgggc aaatgtttcg aacgcacggc
     6181 gagtacttct tccgtgccgt ccatcggcct aatcgcaccc atggcgtcaa ttgaatcaag
     6241 tgcatcaatt gcgtcaattg catccacgcg ccactccacc cccggcagcg cgctccatgc
     6301 ttggtgctgg cggctttcct gctggcgcca ggcgatgccg ccggtttccg agctgccata
     6361 gacttcgatg ggcacgcgcc caagcaaacg tcggcactcc tgggcgacct cgaaccctaa
     6421 cggtccaccc gacgagaaca ccgcgcgcag ccgctccgcc gcctcggccc aacggggatt
     6481 ttccgggata cgactaagat gggccgggct ggaaaccaat agcgcgtcac gcgccgccaa
     6541 gttggccgag agatcttcga accacgagac ggtgctcgcc tgcatccgcc gaccggcggt
     6601 gagcggccac aaaacattga ataataggcc gtaaatatgt tgatgcgaca ccgtggaaat
     6661 gacatccgcg gcgccgagca acgcgccaaa ttgtttttcc agatgcgcca cctcggccac
     6721 caactggtcg agatttttag cgaccggctg agccgcgccg gtgctgccgg aagtgtagag
     6781 tacgagagcg gtaaaatctc cgtcgatggc agcgaagcga tcgggttggt gatgccggtt
     6841 ccctgctggc gccgctttgg gctgccactc ggcagcgaat tcgccgagga agccggctac
     6901 gttgccgcgc aaaccagcgc aagtgccggg caagttgtcg cccggcagca aaactgtctt
     6961 gcgcgccagc caagcgccaa acagcgccgc ggcgaactcg atggcgtcat ggtgccacag
     7021 cgcaaaggcg tcacccggtg attcgccgag cagaacagac cagtcagtga cacgagcatg
     7081 aaactcgctc caagaaaccg ccacgccgcc acgccagccg ataatctggt cggctgagcg
     7141 gctggcattt agcgtactaa gcagattgag cggatgagcc atggcggcgc ttgaaataat
     7201 agcgggcgca atattcgccg gcaaagataa tccccatcag taaataggcg agaaagccat
     7261 tgtagaatga ccactgtgcc atggagccaa agagcgcggt gtaaagcgcg atggcgccgt
     7321 tgagcgcgaa aaatacacac cacacctgcg tgacccggcg cgtgtaggcg acggcagcag
     7381 gcgaaagccc tggttcgtgc agtcgcgcta accgttcaat ggttgacggc ggcaccacca
     7441 ggctgtaagc gaataccgtc aagaatgccc cgctaaccag taccggatag aatctcagtg
     7501 gcaataaagc accggaccaa aacgtcgaga gcgccaacag aatcgtagca ccggtccagt
     7561 agcgcgtcgc cccggtcggg gcggtcgccg gcaagcgcgc cagacctccg agcaacaaca
     7621 gtgcggccaa ccacgccggc gccaccctgc cttcgccgag ccagagcgcc aatggaaaaa
     7681 ccagcgtaat caggatggcc aatatccgaa gcgcgccgcg catggggatt tattgctcga
     7741 tcaactcggc caacgcctcg accacgtcgc ccaccgtacg cactttcttg aacacctccg
     7801 gctgtagtcg tttgccggtg agctgcttca accggaccgc gagatcgact gcatcgatgc
     7861 tatcgatgtc gaggtcgttg tataaatgcg cgtccagcgt gatcttcgcg gcatcgagct
     7921 cgaacatctc gtgcagcata ttgaccagcc acggatatat ttcttccttg cggactggaa
     7981 gcgccgaagt cattggctct gttcctcgtt tttccgaacc gtttcgatca tctcagcgag
     8041 ggcgcgcacc gacgcgaagt ggcggcgatt ttcctccgcc ttggccgata aaataatgcc
     8101 ataacgtttt tgcagcgcca cgccaagctc caaagcatcg atggagtcca gccccagtcc
     8161 ttcgacgaac agcggcgcct gggcgtcgat gtccgccggc gcgatgtcct cgagctggag
     8221 gacatcgata atcaattgct tgatatcttc ttcagttact cgcacgggat tcaccttcct
     8281 gagcaaaaat ctggagcaga tgcgcggtca tccggcgcgc cgcgagcacg tgattggcgg
     8341 cgccttcgac aaacggaccg atggcgatat cttccatcac gtcgaagtga aactgcgagg
     8401 tttgcgccgg caagcgccac cagggttcgc ttttgatcaa cattggcggc gcacagcgca
     8461 ccagcaccgg agtgatgtcg catggcgcgc gcactgcgat gttcgccgcg ccccgtttaa
     8521 gacttatcga gccgtcgggc ggggtgcgcg tcccttccgg aaagacgatg agattgccgc
     8581 cgccgcgcac cgaagcgaca cagtcctcga ccaaacgaac gctgtcaccg cggttacaaa
     8641 tatagcccgc ggcctttacc gtcgcccggg tgaaaggatt gcgccaaagc ccttgtttca
     8701 cgatacaatc ggcccgcggc acaaacgcca tgaggaaaac aatatcgatc aatgtgggat
     8761 ggttggcaag gatcagcagc ccctggcgct cgagccgcgc caacccgttg gcttgatact
     8821 ggaacacgcc gaaggcgcac atcgagcgca cgatgccgag gaaggcaaga cgaatcaggt
     8881 cccgggccac ggcttgacgc cgttgacgtt gccagatgat gagccgaagc agcgggaaaa
     8941 tcaccagccc gccaatgccg atggcgatga agctcagcgc gacgccaaac actcgccagc
     9001 accattcgat acgcttaaac atcccacgac caacgccagc gccttcgctg gcagacatga
     9061 tccagagagt ggtccccgtg aaaataaaag cgcaatatct ccagaccggg agaataacgc
     9121 tcggtgctga tgacttcat
//
LOCUS       DASQKS010000621         4762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3298559, whole genome shotgun sequence.
ACCESSION   DASQKS010000621 DASQKS010000000
VERSION     DASQKS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298559"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1295
                     /locus_tag="VE170_16030"
     CDS             318..1295
                     /locus_tag="VE170_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17001.1"
                     /translation="MQKRRVIRHALIVLGLLLPLQTAHGASTPIKVTIHVPSKSLSIM
                     PYYFGKDKGFFAAEQIEPQLIIMAPPTAIAAMAAGELDFSSTTGAATSAIMRGLPLRR
                     VFYVQQDPVHVLLAQPEIKSVQELVGKTVGVNALTDATGMSTSAILRAHGIDAGKTTL
                     LALQVTDNMIKALTSKRVAAVILPPPYVEELEAKGYVKIAEARTYAPLSFIGLVSAQE
                     TMKKSPQKVQGMVAALHRTMNYILNPANRNEVVQYVSTYHKIDLPLAEKAMAAQMLGY
                     SKDGTKPRAAVEKEIEIYRESLKVAKAFTPDDLEDMSWLRKAQGAVPAR"
     gene            complement(1329..2351)
                     /locus_tag="VE170_16035"
     CDS             complement(1329..2351)
                     /locus_tag="VE170_16035"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17002.1"
                     /translation="MNLRIPAILLALFCAAPSFASHYLPKPGDKPINVRIGTCAITGG
                     FVHLYAGQDYGIFEKYGLKTETIFIRGSGPALAALASDELQFIFCAADGTIPGMATGI
                     DVKLVASPLVKLPYVLISRKEVRRGEDLKGKSIGIARAGDLSDKLTRAVIKKFGLTPE
                     MVSIRPIGGSQGERYQAMAANIVQAVTVTPPLDARAKNAGFNVLYRLIDLDLPFIYST
                     LHTSHKMLRDRPEIVQRAVAAFAESVYFVEKNPDKAKASIAKAMNLKDEDALQSSYNV
                     YAKEIVDRTMLVPAKSVAESVESARESGTTIRRKPEEIFDNSFVNNLEKSGFLKELWG
                     SENYRR"
     gene            complement(2401..3420)
                     /locus_tag="VE170_16040"
     CDS             complement(2401..3420)
                     /locus_tag="VE170_16040"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17003.1"
                     /translation="MKLAILLVLLVPFGAQPFANPYLPKPGERPLSMRVSTCAVSGGF
                     IHLYTALDNGLFDKYGVKLENIFIRGSAQNLTALATDEVQFTYCASDAIIPGMAAGLD
                     VKIVASPLVKLPYVLVTRKDVRRPEDLKGKSLGIARAGDLSDRLSRAVVKKLGVPPDT
                     VTIRPIGGSQGERYQAMAAGIVHGVIITPPLDVRAKNEGFNVMYRLIDLNMPFIYSSL
                     HTSHKMLRERPEIVQRMVAALAEIVYYVEKNPDKAKASVGKAMRTKDPEALQSAYDTY
                     AREILDRTMVVPAKAVAETVEMARESGSPVRRKPEEIYDNSFVNNLEKSGFLKEIWGS
                     ENYRR"
     gene            complement(3423..4451)
                     /locus_tag="VE170_16045"
     CDS             complement(3423..4451)
                     /locus_tag="VE170_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17004.1"
                     /translation="MKLRLAIALFLFTSLTALNAFANPYLPKPGDRPIAIRLGTSAVS
                     GGFMHLYAALDYGIFEKYGFKTEHIYIRGQSPALAALANDELQFNYGAAEGSLPGLAA
                     GVEVKVVASPLVKLPYILVGRKDIRRPEDLKGKAIGVTRPGDLSARLSRAVLKKFGLS
                     TDEVIIRPIGGSQTERYQAMLGNVVQAILVTPPLDVRAKQDGFNVIYRLIELELPFIY
                     SSLMTNYKMLRERPEIVQRMVAALAESIYFVEKNPDKAKAAVAKAMRVKDEEALQASY
                     NVYAREIVDRTMVVPGNSVREAVEIARDTGTVVRRKPEDLYDNTFVNNLDKSGFLKEI
                     WGNENYRR"
BASE COUNT          992 a         1260 c         1464 g         1046 t
ORIGIN      
        1 ctaggggcgg gtttgaaaca cgcctttttc caatcttccg tacctttcct gtccttcgtg
       61 atctcctttg aaaatacctg tgaggggata acatgcacag agcctccggg gttccgtaat
      121 acccgcgaaa tcggtagcca ggctaagtcg acgcagaaca aacctggaat tccgcgtata
      181 cgggaatgac aaatcgagga ggggagcgtg ttttgggaat gccacccatg ttcatgcgtt
      241 gtcggcgagg gcaacgctca cgccacttcg tggtgaatca gaattttttc atggagcgag
      301 agaggttagg taagccgatg caaaaacgaa gagtaatacg ccatgcgttg attgttctcg
      361 ggctgctgtt gccgttgcag acggcgcacg gagcctccac gccgatcaag gtgacgattc
      421 atgtgccgtc caaaagcttg tcgatcatgc cgtactactt cggcaaggat aagggctttt
      481 ttgccgccga gcagattgag ccgcagttga ttatcatggc gccgccgacg gcgatcgcgg
      541 cgatggccgc gggcgagttg gatttttcct cgaccaccgg cgcggcgacc tcggcgatca
      601 tgcgcgggtt gccgttgcgg cgcgtgttct acgtccagca agacccggtt cacgtgctct
      661 tggctcaacc ggaaattaaa tcggtgcaag agttggtcgg gaagaccgtc ggcgtgaacg
      721 cattgaccga tgccacgggc atgtcgacca gcgcgattct acgtgcccac gggatcgacg
      781 ccggcaagac gacgctactc gcgctgcagg tgacagacaa tatgatcaaa gcgctcacga
      841 gcaaaagggt tgccgcagtc atactgccgc cgccctatgt tgaagagttg gaagccaagg
      901 gctatgtcaa aatcgccgaa gcgcgaacct atgcgccgtt gagcttcatc ggcttggtga
      961 gtgcgcagga aaccatgaag aaaagtccgc agaaagttca gggcatggtc gccgcgctgc
     1021 accggaccat gaattacatt ctcaatccgg cgaatcgcaa cgaagtggtt caatatgttt
     1081 cgacctacca caagatcgac ctgccgctgg ccgagaaggc catggccgcg cagatgctcg
     1141 gctacagtaa agacggcacc aagccgcgcg ctgctgtcga aaaggaaatc gagatctacc
     1201 gcgaatcgct gaaagtcgcg aaggctttca cgccggatga tctggaagac atgagttggt
     1261 tgaggaaggc gcagggagcc gtgccggcgc ggtagaattt aaatgaatgg cggctctgtt
     1321 gcgcgtccct agcgccggta gttctcgctg ccccagagct ctttgagaaa cccactcttt
     1381 tccagattat tgacgaagct gttgtcgaag atctcttccg gtttacgccg gatcgtggtg
     1441 ccgctctcgc gggcgctttc gaccgattcg gcgaccgact tggcggggac tagcatcgtg
     1501 cggtcgacga tttctttggc gtagacgtta taggaggact ggagcgcatc ttcgtccttg
     1561 aggttcattg ccttggcaat ggaggctttg gccttgtcgg gattcttctc gacgaagtag
     1621 accgattcgg cgaatgccgc gaccgcgcgc tggacgatct cgggacggtc gcgcagcatc
     1681 ttatggctgg tgtggagggt gctgtagata aacggcaggt cgagatcgat caggcgatag
     1741 agcacgttga agccggcgtt cttggcgcgg gcatccaagg gcggtgtgac ggtaaccgct
     1801 tggacgatgt tggccgccat cgcttggtag cgttctccct gactgccgcc gatgggacgg
     1861 atcgaaacca tttcaggcgt caagccgaac tttttgatca ctgcgcgggt gagcttatcg
     1921 ctcaagtcgc cagcacgggc gatgccgatg gatttacctt tgagatcttc gccgcgtctg
     1981 acctctttgc gtgagatcaa tacatagggc agcttcacca aaggcgacgc cacgagtttg
     2041 acatcgatgc cggtggccat tcccgggatg gtgccgtcgg cggcgcaaaa tatgaactga
     2101 agctcgtcgc tagcgagggc ggccaacgcc gggccgctgc cgcggataaa aatcgtctca
     2161 gtcttgagtc catacttttc gaaaattccg taatcctggc cggcgtaaag atggacgaag
     2221 ccgccggtga tggcgcaagt gccgattcta acattgatcg gcttatcgcc cggcttgggc
     2281 agataatggc tggcgaagct gggcgccgcg caaaagagcg ccaacaggat tgcggggatt
     2341 cttaggttca tcggaatgtt cccatgcctt gcgcgtgggt cgatgccagc gcgccgtaga
     2401 ttaccgtcga taattttcgc tgccccagat ctctttgaga aaaccactct tctccagatt
     2461 gttgacgaag ctgttgtcgt agatttcttc cggttttctt ctgaccgggc tgccgctttc
     2521 gcgggccatc tcgacagttt cggcgaccgc cttggccggg acaaccatcg tgcggtcgag
     2581 aatttccctg gcgtaggtgt cgtaggcgga ctgtaatgct tcgggatcct tggtgcgcat
     2641 ggctttgccg acggacgcct tggccttgtc gggatttttc tcgacgtaat agacgatctc
     2701 ggccagggcg gcgaccatgc gctggacaat ctcggggcgt tcgcgcagca ttttatggct
     2761 cgtgtggagc gaactataga tgaacggcat attcagatcg atcaggcggt acatgacgtt
     2821 gaaaccttcg ttcttggcgc ggacatcgag tggcggcgtg atgatcacgc cgtggacgat
     2881 accggctgcc atcgcctgat aacgttcgcc ctggctgccg ccgatgggcc ggatcgtgac
     2941 cgtgtcgggg ggcacgccga gcttcttaac gaccgcgcgc gacagccggt cgctcaaatc
     3001 cccggcgcgg gcgattccca aagatttgcc cttgagatct tccggccgtc tcacatcttt
     3061 ccgggtcacc aagacatagg gcagctttac cagaggcgac gcgacgattt ttacgtcgag
     3121 cccagccgcc atcccgggaa tgattgcgtc cgaagcgcag taagtgaatt gcacttcgtc
     3181 ggtcgcgaga gcggtcaagt tttgggcgct gccgcggata aagatattct ccaatttgac
     3241 gccgtacttg tcgaacaggc cgttgtcgag ggcggtgtag agatgaatga agccgccgga
     3301 aacggcgcag gtagagacgc gcatcgaaag cggccgttcg cccggtttag gcagataagg
     3361 attggcgaat ggctgagcgc caaacgggac aagcagtacc agtaggatcg ctaatttcat
     3421 tttcaccgcc ggtaattttc atttccccaa atctctttga ggaagccgct tttgtctaaa
     3481 ttattgacaa aggtgttgtc gtaaagatct tccggtttgc gcctgacgac ggttccggta
     3541 tcgcgggcga tctcgacagc ctcgcgcacg gagtttcccg gcacgaccat ggtgcgatcg
     3601 acgatctctc tggcgtagac gttgtaagag gcttgcagcg cttcttcatc tttgacgcgc
     3661 atcgccttgg cgacggcggc tttggccttg tcaggatttt tctcgacgaa gtagatgctc
     3721 tcggccagcg cggcgaccat gcgctggacg atctcgggcc gctcgcgcag cattttataa
     3781 ttggtcatca gcgaactgta gataaacggc agttcgagct cgatcaagcg atagatgacg
     3841 ttaaagccat cttgcttggc gcggacatcc aacggcggag tgacgaggat agcttgcacg
     3901 acgttaccga gcatcgcttg atagcgttcg gtttggctgc cgccgatcgg ccggatgatg
     3961 acttcgtcgg tgcttaagcc gaatttcttc aacaccgccc gggacaggcg cgcgctcaag
     4021 tcgccgggcc gggtgacgcc aatggccttg cctttgagat cctccggcct gcggatatct
     4081 ttgcggccga ccaggatata gggaagtttt accagcggag atgcgacgac tttgacctcg
     4141 acgccggcgg ctaggccggg taggctgcct tcggcggcgc cgtagttgaa ctggagctcg
     4201 tcattggcca acgcggcgag agccggcgat tggccgcgta tgtaaatatg ttcagttttg
     4261 aagccatact tttcgaaaat cccgtaatcg agggcggcat atagatgcat gaaaccgcct
     4321 gaaaccgcgg aggttccgag gcggatcgct atcggacgat cgccgggctt cggcaggtag
     4381 ggattggcga aggcgtttag cgcggtcagc gaggtgaaca ggaatagtgc gatggctaag
     4441 cgcaacttca tggcgccgac tctaagcgga atcgcccggg gttgtaaagg agaaaatctt
     4501 cggcttaatc tagcggacag ccgtggccta tgtagaaaag gttgtgctgt cggaccatcg
     4561 caatcgcgat ggatgaaatg gcggacaatc ggcaagagtg tcctgggaac ttaaggagat
     4621 gcaaatgact gcaaagaaat ggcgcgggtg cgccgtgatt gtcattagcg tagccgtgct
     4681 gtttgggcct aggccggcga cccaagcaca gacgcagaag attcgcatct cgctatcgag
     4741 ccggagcaat accaacacca gc
//
LOCUS       DASQKS010000622         7914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5803585, whole genome shotgun sequence.
ACCESSION   DASQKS010000622 DASQKS010000000
VERSION     DASQKS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5803585"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="VE170_16050"
     CDS             complement(<1..1836)
                     /locus_tag="VE170_16050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HYJ17005.1"
                     /translation="MQLAKTYSHIDIESKWYRHWVDKKLFAPSAGAGEYYSIVIPPPN
                     VTGSLHMGHALNNTLQDILCRYKRMDGFSVLWVPGTDHAGIATQNVVERQLAERGTSR
                     AQLGREKFIERVWEWKKEAGDAILHQLRALGVSCDWSHERFTMDEGLSLAVREAFVKL
                     WEDGLLYRAQRLINWCPRCNTALADIEVIHEEADGNLWHIRYPLADDSGEFLIVATTR
                     PETMLGDTAVAVHPEDARYQHLVGKKIRLPITGRIVPLIADAYVDKEFGTGALKITPG
                     HDFNDFEIGERYNLDRISIFDADARIDGAAFLARGETGDWIERYHGKERSDARKLVIA
                     ELAENDFLQKTEPHKLAVGRCYRCQTVIEPYLTPQWFVNIQPLAEPALQAVRDGRIKI
                     VPEGWCNSYYAWMENIKDWCISRQIWWGHQIPAWYCKNCESANLVQNADGDYLFSKDA
                     KPIVSRMSPSGCPQCGGKDLIQDPDVLDTWFSSGLWPFSTLGWPERTEDLQRFYPTST
                     LVTGFDILFFWVARMIMMGLKFMDDVPFRDVYIHALVRDEQGQKMSKSKGNVIDPLDI
                     MSKYGTDAFRFTLTAMAAMGRDIKLAEDRIGGYQSFVNKLWNAARF"
     gene            complement(1855..2319)
                     /locus_tag="VE170_16055"
     CDS             complement(1855..2319)
                     /locus_tag="VE170_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17006.1"
                     /translation="MLRYAMVVALTPDHGIQFFDSLPESSALAEFDAAIVDAATLRLA
                     ETPPLFDSKAIEHWQVPTLWIDDMETSVPAARANWINLRPPVQRDQLLKALFECLNPP
                     GGVPPAARKLEQRAAMPVKARAKKSQEAAAAPPAAANVIELVDVVEDEEADS"
     gene            2432..2518
                     /locus_tag="VE170_16060"
     tRNA            2432..2518
                     /locus_tag="VE170_16060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2466..2468,aa:Leu,seq:cag)
     gene            complement(3043..3741)
                     /locus_tag="VE170_16065"
     CDS             complement(3043..3741)
                     /locus_tag="VE170_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HYJ17007.1"
                     /translation="MHEFTIFFLRWIHFLAGVTWIGILYYFNFVQTPFFAETEAPVRI
                     GAIQKLLPRALWWFRWGAMVTFLAGILIYLMRLGEMGGSVFFSSPYGVTITLGGLMGT
                     VMFLNVWLVIWPNQQIVMASTNQVASGGQALPAAAGAGRRGALASRTNTVFSIPMLFF
                     MGAASHYGAMVHQSGSRGLYWLLVIVIIGAVEANALVGTQGATKKPLDSVSGALWSGF
                     LLTGIFFLLTLALT"
     gene            4145..5062
                     /locus_tag="VE170_16070"
     CDS             4145..5062
                     /locus_tag="VE170_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYJ17008.1"
                     /translation="MVEANLARAIRSREQQLTEISGHLIVGGGKRVRPMVTLLAYAAF
                     GGNRIRDIVDIATAIELIHTATLLHDDIIDGADTRRGKISAYKKFGLKSTLVAGDFLF
                     IKAFEFAGKFDETVVQWTADACTSLTEGEILQSHFNRNRAVTVDDYIEIVKRKTASLF
                     HTGTKVGAYLAGASQAMVDETERYGLNMGIAFQMVDDILDVVGHPDLLGKPIGMDLRD
                     GNPALPIILALKYGDPAVCAAFDCDNPSETQIDLAIQAIKSSPAIAEAKAMAKNAAHA
                     ALKSIRKLPPSQYRNGLKTLVQLIIDRDF"
     gene            5127..5861
                     /locus_tag="VE170_16075"
     CDS             5127..5861
                     /locus_tag="VE170_16075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HYJ17009.1"
                     /translation="MSVQSHPRDQAVQGMFDRIAGRYDLLNRVISFKLDNRWRKRAIE
                     TLVQGNTPLIVDLGTGTGDMVFSAMKFARGNARIVGLDFSLNMLQLAQTKRAHQPFGQ
                     RNTFVQGSAMASPFKSNAFDGAMSAFVLRNVSDLGGFFSEAYRVLKPGGKLVTLDMFP
                     PRKNWFSPLYCVYFYRMVPWIGGLLAGDRSAYLYLANSVRQFHPPEVVCDKLRQAGFA
                     QVALRKFLNGAVCMHIAEKARGEAAP"
     gene            5866..6696
                     /locus_tag="VE170_16080"
     CDS             5866..6696
                     /locus_tag="VE170_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17010.1"
                     /translation="MQGFSSSFLIFFYQIALGGMFALAGTPFHEIERGFYKSTAGVLY
                     FIALLGFFGKCVFYLHAFSQPISLALGFEIFFHSAFVTTFTGYMISLWGERQVFRARS
                     FAATVLSGLAGLLCTTASFSSAPLLSVETVIYPMAFFLSSLLLGGVTVGMLIGHWYLI
                     DTGQSLEPFIRIYKFFIYALFAQCLFYLVAGPLLYWAGSAASFTALERLWQRHSTLLI
                     TRLMAGQVAPLILSWMIWRTLKIPHTMAATGLFYIALLGVFVGEILGRQILALTALPF
                     "
     gene            complement(6714..6977)
                     /locus_tag="VE170_16085"
     CDS             complement(6714..6977)
                     /locus_tag="VE170_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17011.1"
                     /translation="MAEADKDPLLSNRGDMYHQGVVTKLFPSNNTGVVRTESGRELTF
                     SYDLVDLVGAVKSALDLHVGEPVGYDLGWTSAGLRVTKIKTYR"
     gene            complement(7061..7543)
                     /locus_tag="VE170_16090"
     CDS             complement(7061..7543)
                     /locus_tag="VE170_16090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HYJ17012.1"
                     /translation="MNFGQDRLLADSVVLTRLDKALGWARKYSLFPYPFATACCAMEY
                     MALSMSPYDIDRFGALLPRFSPRQADLLMVIGTVNHKLSPVLKRVYEQMSEPKWVMAF
                     GACAASGGFYDNYATVQGIDRIIPVDVYVPGCPPRPEAVLDGLMELQGRIAQQKHPVT
                     "
BASE COUNT         1811 a         2261 c         2049 g         1793 t
ORIGIN      
        1 gaagcgcgcc gcgttccata acttattgac gaagctctgg tagccgccga tacgatcttc
       61 ggcgagcttg atgtcccgcc ccatcgccgc catcgcggtt aatgtgaagc ggaaagcgtc
      121 cgtgccgtac ttgctcatga tgtctagggg gtcgatgacg ttgcccttgg acttggacat
      181 cttctgcccc tgctcgtcgc gcaccagggc gtgaatataa acatcgcgaa acggcacgtc
      241 gtccataaac ttgagcccca tcatgatcat gcgcgctacc caaaaaaaca aaatatcgaa
      301 gccggtcacg agcgtggaag ttggatagaa ccgctgcaga tcttccgtgc gttccggcca
      361 tcccaacgtc gagaacggcc ataggccaga ggaaaaccag gtgtcgagaa catccggatc
      421 ttggatcaaa tccttgccgc cgcattgggg acagccactg ggcgacatgc gcgaaacgat
      481 cggcttggcg tcttttgaaa ataaataatc gccgtcggca ttctgcacca ggttcgccga
      541 ttcgcaattc ttgcaatacc atgcgggtat ctgatggccc caccatatct gccgcgagat
      601 gcaccagtct ttgatgttct ccatccaggc gtagtaggaa ttgcaccagc cctcgggaac
      661 gatttttatc cgcccatcgc gcaccgcttg cagcgccggt tcggccagcg gctggatatt
      721 cacaaaccat tggggcgtga gatatggctc gatcaccgtc tgacagcgat agcagcggcc
      781 aacggcaagt ttgtgtggtt cggttttttg cagaaagtca ttttccgcca actcggcgat
      841 cacgagtttg cgcgcgtcgg agcgctcctt gccgtggtaa cgctctatcc aatcgcccgt
      901 ttcaccgcgg gccaagaatg ccgcgccgtc gatacgggcg tcggcgtcaa aaatactgat
      961 gcgatccaag ttatagcgtt caccgatttc gaaatcgttg aagtcatgcc cgggcgtgat
     1021 tttgagcgcg ccggtgccga actctttgtc gacataagca tcggcaatca acggcacgat
     1081 tctgccggta atcggcaagc gaattttttt accgaccaag tgctgatagc gcgcatcctc
     1141 gggatggacc gccaccgccg tgtcgccgag catggtttcc ggccgtgtcg tggcgacgat
     1201 caggaattcg cccgagtcgt cagccagcgg atagcgaata tgccaaagat tgccgtcggc
     1261 ctcttcatga atgacttcga tatcggccaa ggcggtgtta cagcgcggac accaattgat
     1321 cagccgctgt gcccggtaaa gtaagccgtc ttcccaaagc ttgacgaacg cctcgcgcac
     1381 cgcgagggac agtccctcgt ccatggtaaa tcgctcatgg ctccagtcgc aggagacccc
     1441 gagggcgcgc aattggtgca agatcgcgtc accggcttct tttttccact cccacacccg
     1501 ttcgatgaat ttctcccggc ccaactgcgc ccggctcgtg ccgcgctcgg ccaattggcg
     1561 ctcgacgaca ttttgcgtcg cgatgccggc atgatcggtg cccggcaccc agaggacgga
     1621 gaagccatcc atgcgcttgt agcggcagag aatatcttgc agcgtgttat tgagcgcatg
     1681 acccatgtgc aaagagccgg tcacattggg cggcggtata acgatcgaat agtattcccc
     1741 cgcgccggcg ctgggcgcga ataatttttt atccacccaa tggcgatacc acttggattc
     1801 gatatctatg tgcgaataag tcttggcgag ttgcatattc ctttattcca gcgctcagct
     1861 gtccgcttcc tcgtcttcca ctacatcgac cagttcaatg acgttggcgg cagccggcgg
     1921 cgccgcagcg gcttcctggc tcttcttggc gcgcgccttg accggcatgg cggcgcgttg
     1981 ttcaagcttt ctcgctgccg gcggcactcc tcccggtgga ttgagacact cgaacagtgc
     2041 tttgagcaac tgatcgcgct gcaccggcgg tcttagattt atccaattgg cgcgcgccgc
     2101 cggcacggac gtttccatgt cgtcgatcca cagcgtcgga acttgccaat gttcaatggc
     2161 cttggagtcg aacagcggcg gcgtctcagc caaccgcaac gtcgccgcat cgacgattgc
     2221 cgcgtcgaat tctgctaacg ccgatgattc cggcagggaa tcgaaaaact ggatgccatg
     2281 atccggggtc aacgccacca ccattgcgta acgcagcatc aggctcggtt ctatgactaa
     2341 taatcgactc atttcggacc atcacgccgg tcaaacttga caaccccata cccccaagtt
     2401 agaaatatct ggttgtcatt gagcttagtg cgcgaaagtg gcggaattgg tagacgcgca
     2461 ggactcagaa tcctgtgggg taacagccgt gggggttcga ttcccccctt tcgcaccaac
     2521 aattatttaa catacaagcc agcgattctc taacgttcgc cgatttcatc ctgccaagaa
     2581 cgtcgcgttt aatccggcta cgctactcgt gggctaaacc gtctgggcgc acaaaggatt
     2641 ccgcggaagc agcgagctgc ggtgaatcat cgctgtaatg agtgggagcc ccttatgccc
     2701 aggatcggtc agttcatcgt caccgctcta ctcctgagca gcgtcgccgg ctgctcaaaa
     2761 gatagcgatc aaacttcaga aaaaagccca gattggcaac gaggcagggc cgtctatctg
     2821 gctaactgtg ttgcttgcca tggtaacgac ccttcgaagg acggtcccat cggacccgcg
     2881 attgccggct cgtctaggga attactcgaa cgccgagtgc tctccaccag ctatccgccc
     2941 aattacaaac caaagcggcc aactaaggtc atgccccaat tcccatttct gaaagaggag
     3001 ataccttatt tggccgctta cctggcgaaa taatttacgc gtctaggtga gcgcgagggt
     3061 taacaggaaa aaaatcccgg tcaagagaaa gccggaccac agggcgccgc taacggagtc
     3121 taacggcttt ttggttgcgc cttgggtgcc gaccaaagcg ttggcttcga cggccccgat
     3181 aataacaatc accagcaacc agtagaggcc gcgagaaccc gattgatgca ccatggcacc
     3241 ataatgactg gccgcgccca taaagaagag catgggaatg gaaaacaccg tgttggttct
     3301 ggatgccaaa gccccgcgcc ggcccgctcc cgccgccgcc ggcaaggctt gtccacctga
     3361 agcaacctga ttggttgaag ccatgacgat ctgctggttg ggccaaatca ccaaccacac
     3421 gttaagaaac atcactgtcc ccatcagtcc gcccaaggta atcgtcacgc cgtagggcga
     3481 tgaaaagaac acactgccgc ccatctcacc gaggcgcatg agatagatca gaatacccgc
     3541 cagaaacgtg accatagcgc cccagcgaaa ccaccacagg gcacgcggta aaagtttttg
     3601 aatcgcgcca atacgcacag gcgcttcggt ttccgcaaaa aaaggagttt ggacaaaatt
     3661 aaaatagtat agaattccaa tccaagtcac gccggctaga aaatgaatcc accgcaagaa
     3721 aaatatagta aattcgtgca tcgctgatct ctcctctcgt cacatcgttt gaactgtatg
     3781 ctgaaaaaca aaccgagtcg gtatgatgcg tttatatatt cggggatggt catttttgtc
     3841 aaggaatatt cacgtctttt gcagtaggcg gtagctttct tggcgccgca gcacggccga
     3901 ttcacagaca gcgatcgaca caacacaagc gtcacggtga ccagcacgat tgcttgacct
     3961 gactagggat ctatgacatt ttccctgcta acgtttctgg ccggcccgtg gagattatct
     4021 gatccaagaa agcaaatccg gaacgctggc ggcgcaatcg gccgccccgt ccgatggtga
     4081 acggaaaatc ccgctactga cgggactcaa cgatccgttc aactacgtca gcgacgaact
     4141 caatttggtc gaagcgaatc ttgctcgggc gatccgctcg cgcgaacagc agctcaccga
     4201 aatctccggt catctgattg tcggcggcgg taaacgcgtc cggccgatgg tgacgctcct
     4261 ggcttacgcc gccttcggcg gcaaccggat tcgagacatc gtcgatatcg ccacagccat
     4321 cgagttaatt cacaccgcga cattgctcca tgacgatatt atcgatggcg cagatactcg
     4381 ccgtggcaag atttccgctt ataagaaatt cggcttgaaa tcgacgcttg tcgccggcga
     4441 ctttctgttc atcaaagctt ttgaattcgc cggcaagttc gatgagaccg tggtgcagtg
     4501 gaccgccgat gcctgcacat cgctgaccga aggcgagatt ctgcaaagtc atttcaaccg
     4561 caatcgcgcc gtgacggttg acgattatat agaaatcgtc aagcgcaaaa ccgcatcgct
     4621 atttcacacc ggcactaaag tgggcgccta tcttgcaggt gcgagccaag ccatggtcga
     4681 cgaaaccgaa cggtatggcc tgaacatggg catcgccttc cagatggtag acgacattct
     4741 cgatgtggtc ggccacccgg atctcctcgg taagccgatc ggcatggacc tgcgcgatgg
     4801 caatccagca ttgccaatca tcttggcact caagtatggc gacccggcag tatgcgcggc
     4861 gttcgattgc gacaacccgt ccgaaacaca aatcgacctt gccatacaag cgatcaaaag
     4921 cagtccggcc atcgccgagg ccaaggccat ggccaaaaat gcggcgcacg ccgcgctgaa
     4981 atcaattcgc aaactccccc cttcgcagta tcgaaacggt ttaaaaaccc tggttcaact
     5041 catcatcgat cgagactttt agaaagccgc aacggcttat gccagcgaca gcgctgctgg
     5101 cctatgctga ttttgcattt caattcatgt cagttcaaag tcatccgcgc gaccaagcgg
     5161 tgcagggaat gttcgaccgg atcgccggcc gctacgattt gctcaatcgc gtcatcagct
     5221 tcaagctcga caatcggtgg cgcaagcgcg caattgaaac ccttgtccaa ggtaatacgc
     5281 cgctgatcgt ggatctcggc accggcaccg gggatatggt ctttagtgcc atgaaattcg
     5341 cgcgagggaa tgcccgcatc gtcggccttg atttttccct gaacatgctg caactcgcgc
     5401 aaaccaagcg cgcgcatcag ccattcggcc agcgtaatac gttcgtacag ggcagcgcca
     5461 tggcgtcgcc gttcaaaagc aatgccttcg atggcgccat gagcgctttc gttctacgca
     5521 acgtatcgga cctcggcgga ttttttagcg aggcctatag agttctcaag cctggcggca
     5581 aactagtgac tttagacatg tttccgcctc gaaagaattg gttctcgccg ctctattgcg
     5641 tctatttcta tcggatggtg ccgtggatcg gcggcttgct cgccggcgac cggagtgcct
     5701 acctatatct agccaactcg gtgcgccaat ttcatccgcc agaggtggtt tgcgataagt
     5761 tgcgccaagc cggctttgcc caggtcgccc tgcgtaaatt ccttaacggt gcggtatgca
     5821 tgcacatcgc tgaaaaagcg cgcggcgaag ctgcgcctta aaaacatgca aggtttttcc
     5881 agcagctttt tgattttctt ctaccagata gccttgggcg gcatgttcgc cctggccggc
     5941 acaccgtttc acgaaatcga gcgaggtttt tacaaatcca ccgcaggcgt cttgtatttc
     6001 atcgctctgt tgggattttt cggcaagtgc gttttttatt tgcacgcttt cagccaaccc
     6061 ataagcctgg cactgggctt tgaaatattc tttcacagcg cgttcgttac gacgtttacc
     6121 ggttatatga tttctctttg gggcgaacgg caagtctttc gcgcgcgcag cttcgccgcc
     6181 accgtcctca gcggcctcgc cggcctactc tgcaccaccg cgagtttctc cagcgcgccg
     6241 ttactgtcgg tggaaacggt gatttatccg atggcatttt ttctctcgtc gctgctcctc
     6301 ggcggggtca cggtgggcat gctgatcggc cactggtatt tgattgatac tgggcaaagc
     6361 ctggagcctt ttattcgcat ttacaaattc tttatctatg ccttattcgc gcaatgtctg
     6421 ttttatctgg ttgcaggtcc gctgctatat tgggccggaa gcgccgccag tttcacggcg
     6481 ctcgagcgtc tctggcaacg ccactcgacg ttgctcataa cccggctcat ggccgggcaa
     6541 gtcgcaccgc tgattttatc ttggatgatc tggcgcacgt tgaaaattcc ccacacgatg
     6601 gcggcaaccg gcttatttta tattgcgctg ttgggagttt ttgtcggcga gattttgggc
     6661 cggcagattc tagcactcac cgcgctgccg ttttagcctc gccgacgctg ggcttaacgg
     6721 taggttttta tcttggtcac tcgcaagccg gcggaagtcc aacccagatc gtaaccaacc
     6781 ggttcaccga catgcaaatc aagcgctgac ttgaccgcgc cgacgagatc caccaaatcg
     6841 tatgaaaatg tcagctcgcg gccgctctcg gtgcgcacca caccggtgtt gttcgacgga
     6901 aacagctttg tcacgactcc ttggtgatac atatcaccgc ggtttgacag caacgggtct
     6961 ttatcggcct cagccattga gtgtgcagaa ttgattcgga actatcggca aagcgcggga
     7021 gctgatcact gcacagccgc gcaagctcat ccaaaacgtt tcaggtgacg gggtgtttct
     7081 gttgcgcaat ccggccttgt aactccatga gcccatccaa gactgcctcc ggacgtggcg
     7141 gacagccggg cacgtaaacg tcaactggaa tgatgcggtc gatgccttga accgtcgcgt
     7201 aattgtcgta aaaaccgccg ctggcggcgc aagcgccaaa ggccatgacc catttgggct
     7261 cagacatttg ctcgtaaact cttttgagca caggcgaaag cttgtggttg accgttccaa
     7321 tcaccattag aagatccgct tgacgcggac taaagcgggg caataacgcg ccaaaacgat
     7381 cgatatcgta gggcgacatg gataacgcca tatactccat ggcgcaacaa gccgtcgcaa
     7441 acggatatgg gaacaacgaa tacttgcgcg cccaacccaa tgctttatcc aaacgcgtca
     7501 ataccacgga atcggctaac agccggtcct ggccaaagtt caaatccgcc tgtgtaatcg
     7561 cttgctgtga tggaatcaat taaccacctt caatttcaaa tatttattat taattgaccg
     7621 tactctgtaa ttgaaagtac tctgttttta cggtgttgac aaatcgccct gactaccgag
     7681 tagagttgac gtgctcgtca caaatatgtt tcgtaactca tgaaattgaa actccatgag
     7741 cgggattgca ttacgcgtgt ttgacgcttc gataatcgcc gagctcaaga attcgccaca
     7801 gtcgcttgct aaattggcaa gaacaactat acgaagaata catgaatccc ttatatattc
     7861 agaaattact gatcgatgtt tgagcacctc aatatatcat tccaggcgca agcg
//
LOCUS       DASQKS010000623         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1605380, whole genome shotgun sequence.
ACCESSION   DASQKS010000623 DASQKS010000000
VERSION     DASQKS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605380"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1861)
                     /locus_tag="VE170_16095"
     CDS             complement(302..1861)
                     /locus_tag="VE170_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ17013.1"
                     /translation="MTLLEFFTHNSGELGELILEHLFLVVVSTGIAVLIGVPLGILLT
                     RKPGLSKPILGFANIMQTVPSLALFGFLIPLNIYLFNVKIVGGIGARTAIVALVLYSL
                     LPIIRNTFTGISNVDPAIREAGRGMGMTDRQLLFQVELPLALGVIIAGVRVATVICVG
                     TATIAAAIDAGGLGRYIFRGLRANDNVLILAGAVPAALIALAADLLLGYLEKTIQAGA
                     AVKVSSSKLLLSGAAALLVFLGVAYFVLGKSAGRIAVGSKDFTEQVIIGEILAQAIEA
                     KTGAEVVRRFDLGGNLAHDALIAGEIDLYAEYTGTGLLAILKEKPLADAQEVYRRVKS
                     EYAKRFKLEWTEPLGFNNTFAILVRGDDARKLNLKTVSDAAKVSSQWRAGFGQDFMSR
                     ADGYPGFAKAYGFHFAAIREMDLSLTYRALAENQVDLIAGNSTDGLIPRYGLFQLDDD
                     RRYFPPYDAVPVVRQTALEKYPALREVLKQLAGVLTVDEMRKLNFAVDGEKRQAKDVA
                     REFLRQKQLLH"
     gene            complement(1858..>2044)
                     /locus_tag="VE170_16100"
     CDS             complement(1858..>2044)
                     /locus_tag="VE170_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008226615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ17014.1"
                     /translation="TMVFVTHDIAEAFVLATRIALLKDGEMILLGPPAELLESTDPEA
                     RAFADCFHGGQSVGRPA"
BASE COUNT          467 a          611 c          565 g          401 t
ORIGIN      
        1 ttgccatcgg caacccgccg cgcttggcgg tgaaacccat caagagtggg aaagaacggg
       61 aatgcgagca ggtcagcgcg tttacggtac ggctccggca gaaactccag aagttcggca
      121 tcgctttcga ccacatgtcc gtcggcatcg ataaaacctt ttattttcgt catcgtctca
      181 ccgcctttct gcggcaagcc gcagcaaatt tccaagttcg tttctatgcg ttccgagcag
      241 atctctgcta tgagcaaatt cgacccgtag ccactaaaga aaaactaaac acgctccgta
      301 gtcaatggag cagctgtttt tgccgcaaaa actcgcgcgc cacatccttc gcctggcgct
      361 tctcaccgtc cactgcgaag ttaagcttcc gcatctcgtc gacggtgagg acacccgcta
      421 gttgtttcag cacttcgcgc agcgccggat atttctccaa agccgtttgg cgcaccaccg
      481 gcaccgcgtc atagggcggg aaatagcgcc ggtcgtcatc gagttgaaat aatccgtagc
      541 gtgggatcaa gccatcggtg gaattgccgg cgatcaaatc gacttgattt tccgccagcg
      601 cccggtaggt tagcgacaaa tccatctcgc ggatcgcggc gaagtgaaat ccgtaggcct
      661 tggcaaatcc cggatagccg tcggcgcgtg acatgaagtc ctgaccaaag ccggcacgcc
      721 actgactgga aacctttgcc gcgtcgctga cggttttcaa attgagtttc ctggcatcgt
      781 cgccgcggac gagaatggcg aaggtattat tgaagcccag cggttcggtc cactccagct
      841 tgaagcgctt ggcatactca gacttcactc gccggtacac ttcttgcgca tcggcgagcg
      901 gtttttcttt gagaatcgcc agcaagcccg tccccgtata ttcggcgtag aggtcaattt
      961 caccggcgat gagcgcgtcg tgcgccaagt tgccgccgag atcgaatcgg cgcacgactt
     1021 cggcaccggt cttcgcttcg atcgcttgcg ccaaaatttc cccgataatc acctgctcgg
     1081 tgaagtcctt cgagcccacc gcaatgcgtc cggcactttt acctaaaacg aaataggcga
     1141 cgccgagaaa aaccaagaga gctgcggcgc cggacaatag gagtttactt gagcttactt
     1201 tgacggcggc gccggcctgg atcgtcttct cgaggtaacc caaaagcaga tcggcagcca
     1261 gcgcgatcag cgccgctggc accgcgccgg caaggatcaa aacattatcg ttggcgcgca
     1321 ggccgcgaaa aatgtagcgc cccagaccgc cggcatcgat ggccgcggcg atggtcgccg
     1381 taccgacgca gatcaccgtc gccacgcgca cgccggcaat gatcacgccg agcgccagcg
     1441 gcaattcgac ttggaaaagc agctggcggt cggtcatacc catgccgcgg ccagcttcac
     1501 gaatcgccgg atcgacgttg ctgataccgg taaacgtgtt gcgaatgatc ggcaaaagcg
     1561 aatagagcac cagcgcgacg atcgccgtgc gcgcgccgat gccgccgaca attttcacat
     1621 taaacagata gatattgagg ggaatgagga aaccgaacag cgccaagctc ggcacggtct
     1681 gcataatgtt ggcgaaaccg agaatcggtt tgctcaagcc cggcttgcgc gtgagcaaga
     1741 tgcctaacgg cacgccgatc aacaccgcta tccccgtgga gacgacaact aaaaagagat
     1801 gctctaggat cagctcgcca agctcgccgc tgttgtgggt gaagaattcc agtagcgtca
     1861 cgccggtctc ccgacgctct gaccgccgtg aaaacagtcg gcaaaggcgc gcgcttccgg
     1921 atcggtcgac tcgagtagtt ctgccggcgg cccgagtaaa atcatttcgc catctttcaa
     1981 gagcgcgatg cgcgtcgcca ggacaaaggc ttcggctatg tcatgagtga cgaaaaccat
     2041 cgtc
//
LOCUS       DASQKS010000624        15668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5804053, whole genome shotgun sequence.
ACCESSION   DASQKS010000624 DASQKS010000000
VERSION     DASQKS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15668
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5804053"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..462
                     /locus_tag="VE170_16105"
     CDS             136..462
                     /locus_tag="VE170_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17015.1"
                     /translation="MSNFKKLTDATSFSAEKMKKNGVFETERFFCDTYCFEPGQEQKP
                     HTHANEDKVYYVLEGRGSFTVGSEEREMGAGEIALAPAGQSHGVANRSQSRLVTLVFV
                     TPKPHH"
     gene            473..895
                     /locus_tag="VE170_16110"
     CDS             473..895
                     /locus_tag="VE170_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ17016.1"
                     /translation="MTPEQEAVAQANQNFYRAFETLKIEAMENVWAKDGEIQCGHPGW
                     RILRGWGPVMESWKRIFANTPSMQFTLTDVKIEVRGELAWVTQYENLNSSIEGQTFSA
                     AILTINMFQKTAEGWRMILHHGSSVSQPPDQDDSNTVH"
     gene            1127..1840
                     /locus_tag="VE170_16115"
     CDS             1127..1840
                     /locus_tag="VE170_16115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ17017.1"
                     /translation="MTSTPLEEKTALVTGAGRGIGRAIAVALAKAGCTVVITARTAPE
                     LAAVRQEIVAAKGKASIVQADLTRDDELHALIETCGPVDFLINNAGWGKRATVVRSRV
                     DDWDQTFRVNLRAPMILAKELLPAMIQKGEGAVINIGSVSGKSGEANGAAYSASKFGL
                     IGFTQSLYEEVREQGIKVAVILPGFVDTPMIPPVKHLDRSKMIQANDVAQAVLYILNA
                     PPTACPVEITIRPQRTPYR"
     gene            2005..3873
                     /locus_tag="VE170_16120"
     CDS             2005..3873
                     /locus_tag="VE170_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ17018.1"
                     /translation="MNLPPEQEAIRAKCFHPSGRFVEFPEEDVETSIPERFEKIAARY
                     PDRVAVKLRNRLLTYRELNDTANRLARAILARRGNTQEPIALLMEHDIPLFVAMLGVL
                     KTGKICVVLDPSFPNDRNVFLIEDSQAGLLISDRENLSSAAEYTHGRCGCVNIDELDS
                     SLSIENLRLPIAPMNFAFLIYTSGSTGQPKGVIQNHRNLLHDCLLYCNGLHVCADDRI
                     ALLYSCSASQGLKITFAGLLNGAALYPFNMRQRGVADLPLWLNQEEISIYFSVPVVFR
                     QFTSTLTGHYQWPSLRIIQLGSDLVTLRELEEFQKHFTADTILIIRFGATETGTLRRL
                     YVDAKSSLDQIKNAVGYATEEAEISIRDEADGQMKCDVIGEIVVHSRYISPGYWRRPN
                     LHQDPFFPDPNGRGKRIYRTGDLGRLRSDGLLFHLGRKDFQLSIRGYRVEPSEIEAVL
                     LAQDNVKEALVAAGPGDTQAEHDRLVAYIVPYYKPFPSSPVLRRAARQKLPAHMIPSD
                     FVFLESLPLTPNGKVDRLALPAPDTARSEIEMTYAAPRSDIENRLVKIWTEVLSIEHV
                     GIHDNFFDLGGHSLSATRVLSRIFEQYQLEIPLKSLFQSPTIAELAAQIIAHQKNG"
     gene            4100..5164
                     /locus_tag="VE170_16125"
     CDS             4100..5164
                     /locus_tag="VE170_16125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17019.1"
                     /translation="MSMIDADTHVDECEETWKKLEGTQYAKYIPVTTTMPPEEAKRAG
                     YNPTNSRCWVVEGRLQNRAIRDDINHPPRVFRELQDVSGRVAHMDKMGVAVQVIFPTF
                     FIRYNTSNAEAEWALTTTYNRWIAGKCAQTNGRLRWAAVLPFLSPDKAVEEVRWAKEH
                     GACGIFKRGFDLDRYVTDPHFFPAYEEASAHDLPLCIHTGHPLPGHEWDRGFPIMYSC
                     GAIVSSGIAQKFPKLRFGFIESGASWIPYTISQLGAVKRQTLRGQNTRLPNLYELEPE
                     MFRANRLFVTIDPIDDIESILKFGTEDNIMIGTDYSHTDISANLSALDEVRSWVKEGR
                     INDAQAHKILESNSKEFYRL"
     gene            5312..6076
                     /locus_tag="VE170_16130"
     CDS             5312..6076
                     /locus_tag="VE170_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HYJ17020.1"
                     /translation="MTKKHLPNYYIILRVQPNASVDTIKASFRKLMVTMKMHPDLGGD
                     HDVAAQINEAYAVLKDHSRRVKYDRLYLLQRLEAAQAAAHEREKRAAQGAGARPAAGS
                     TKRPSSSPQTRDGKAGQCPYCNTALPKLIESDTRCERCHSPLSAPPHLSSFGKELFGR
                     RATPRIVKSHFATVHPTGQAQSMTVKMRDLSLAGISFYSEINLDIEQVFKFRDASLEA
                     IAVVVSRGKRGQWYSIHARLLTVAFHQPGVFVSASR"
     gene            complement(6338..7015)
                     /locus_tag="VE170_16135"
     CDS             complement(6338..7015)
                     /locus_tag="VE170_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYJ17021.1"
                     /translation="MLRAVIFDFNGIIVDDEPIHFELFQKVLAEEGIELTNDAYYARY
                     LGFDDRGAFSFGYHEHNRPLSQNKLAQLIERKAVYYQEAIRNHVAIFPGVKKLVVELA
                     QSLPLAVASGALRNEIETILTSANLIHHFKAVVSAEDVKRGKPEPDIFLQALAALNVD
                     TSDAIQAGDCVVIEDSKEGIKGARHAGMKCLAVTNSHPAELLIDADAVVTTLETVTLD
                     DLAGLCR"
     gene            7075..7626
                     /locus_tag="VE170_16140"
     CDS             7075..7626
                     /locus_tag="VE170_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYJ17022.1"
                     /translation="MAADHHQAHHAAHGHGHIDYRFCPKCGGGLDRKTVKASEPARLV
                     CQACSFIFYQDPKVVAGTIFTLNGGIVLLQRGVEPALGKWVFPGGYVDRGESVQDAAV
                     RETREECLLDVKLGPLLNVYSYPRSPNVIVVYTAEVVGGTLTAADESTDAAVFAPTAV
                     PWHELAFDSTRDALNDYIRNYLT"
     gene            complement(7796..8104)
                     /locus_tag="VE170_16145"
     CDS             complement(7796..8104)
                     /locus_tag="VE170_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ17023.1"
                     /translation="MANFVKVCKTGDVKGGCGKSIEIDGKAVGVFNVDGTFYAINDVC
                     GHRGGPLAEGEMDGTTVICPWHGWRYNVTTGENELLPALPTEKYQVQVEGDDVLVDLG
                     "
     gene            complement(8168..9256)
                     /locus_tag="VE170_16150"
     CDS             complement(8168..9256)
                     /locus_tag="VE170_16150"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HYJ17024.1"
                     /translation="MAKRSTLNDLHERRGATFVEKDGWLLPDHFGDPPSEYGSVRQSA
                     GLFDLANRAMLQFTGADRLPYLQGMLSNDVMPLKVFDGQQAAILTQQGKVVADVRVLC
                     SLNSLYLDFWEPLKEQILAHLNRFLVADEVEIHDPNEQWKMLSLQGPRAQAMLRQLFA
                     NAEMPAQANQHGMVQFDDTPVCVVRADRTGYQGFDLIVQTSHLLVFAQRLNALGAAWV
                     GEWAQNILRVEAGIPRYGVDFSEDNLLLEVALDDAVSFTKGCYLGQEVVERIRSRGHV
                     NKKLCGLLLDGDTPASPGDKLSAGAKEVGQITSSVASIALHQPIALGYVNRDCWTPGT
                     KLTVHHDGGDFGATVTARPIVAPAQPGP"
     gene            9375..9839
                     /locus_tag="VE170_16155"
     CDS             9375..9839
                     /locus_tag="VE170_16155"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:9507..9509,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ17025.1"
                     /translation="MAGLEGQKAPAFTLAGSDGKQHSLAEYAGKNVILWFYPKDDTPG
                     UTKEACGFRDAIDTVTKANTVVVGISPDGIDSHNQFITKFNLPFVLLADQQKETMKAY
                     GAWGPRKNAAGEVSEGTIRSTALIGPDGTVKKHWIPVTDPGAHPADVLEFLK"
     gene            9912..10823
                     /locus_tag="VE170_16160"
     CDS             9912..10823
                     /locus_tag="VE170_16160"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HYJ17026.1"
                     /translation="MAKIKADLDLHKLEIFYWVAEHKSFSQAAELLSLRQPTISAHVQ
                     ELETAVGGKLLYRMPGKISLTPLGLMLAERAKTLLAFKRETVAAVEQFHGTLSGELWV
                     GGSSIPGEYLLPQKLGEFIKKFPQVKPILRIGDSAGVVADLLDGKVEIAFVGFKGDDP
                     RLNFEKIWDDEMVVAVPKDHPWTRGKAIQIADLRSERFISREHGSGTLESIQQLLGKR
                     KPPGRGLLNITMELGSTEAVKEALMAGYGFSILSRISIRHELDEGSIVEVPVRGLKLD
                     RGFYQVFHSRRPFHPIAQAFRAFLKLP"
     gene            10923..12281
                     /locus_tag="VE170_16165"
     CDS             10923..12281
                     /locus_tag="VE170_16165"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HYJ17027.1"
                     /translation="MALPLMSRGRRAAEELRERLIDAWARSDKIFAIVGSGQTLHQPI
                     VWRHPFIFYIGHLPAFSWNQICGGILQWSSVNAYYDDLFCRGIDPDVDTGECHWHPEV
                     PEDWPSLGDTLEYRDKVRRALLESTIALGYSRSNDLLAQGGRVFELILEHEFMHQETL
                     LYMMQQMAPGDKIRPHDLPRYSFGRATRAESIRIPAGKARLGAHFEDLSFGWDNEFSE
                     VTVDVESFAMDSLPVTNGAFFEFLYAGGYDEPRYWRPEDWRWRQLERKQSPTCWLEQE
                     GSWWYKAMFDLLPLEQVASWPVSVSLAEARAYAAWRGKRLPTESEYHRAAFYGPEGVE
                     SIYPWGEAGPTPRHGNFDFTAWSPQPVGSHPAGASRWGVQELVGNGWELIDTPFAPLP
                     GFTPYMTTYPDYSKDFFDGKHFVVKGASWATDAGLVRSSFRNWYQAHYPYVFAKFRCV
                     TD"
     gene            12307..12588
                     /locus_tag="VE170_16170"
     CDS             12307..12588
                     /locus_tag="VE170_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HYJ17028.1"
                     /translation="MKAVWENTVVAESNQTIVIEGNHYFPPDSIKKECFAPSDTHSTC
                     PWKGLASYYHVKAGEKLNQDAAWFYPEPKDAAKSIKNYVAFWRGVTVSE"
     gene            12605..13129
                     /locus_tag="VE170_16175"
     CDS             12605..13129
                     /locus_tag="VE170_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HYJ17029.1"
                     /translation="MRTLRIASCMAENTEDFCRAIVEYLQLKLDVPAEYVTGIPWQER
                     EVLFDRGEIQVLWLCGLPYVHKADLAESGMELLAVPIPVGERYGSRPVYFSDIVVRRD
                     SPCRSFRDLRGATWAYNEPRSHSGLNVRAHLFELGEIDSFFAAAVEAGAHSNSLQMVL
                     SGVVDGAAIDSTVC"
     gene            13123..13404
                     /locus_tag="VE170_16180"
     CDS             13123..13404
                     /locus_tag="VE170_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HYJ17030.1"
                     /translation="MLEWLGSQRHDLADEIRVIDTLGPSPIPPWVISRHVAASQRGAL
                     RQLLAELHLDPIGRDILADGRLLRFTAADDRDYDPIRAMAHKADRVSLV"
     gene            13470..13937
                     /locus_tag="VE170_16185"
     CDS             13470..13937
                     /locus_tag="VE170_16185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ17031.1"
                     /translation="MASLEGKKAPPFSLQGSDGKKHSLTDYQGKTVILYFYPKDNTPG
                     CTKEACGFRDLNVSLKKPGTVVLGVSKDSIESHGKFGALYKLPFTLLSDPNAEVMKKY
                     GAFGKKMMYGKEVQGTIRSTVVIDPKGEVIKHWTTVKKAEEHPQAVFDFLNNK"
     gene            14046..15143
                     /locus_tag="VE170_16190"
     CDS             14046..15143
                     /locus_tag="VE170_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HYJ17032.1"
                     /translation="MDRREFLKQGLTASAGLALVGLPKFTGWSHADDPTKWRSFEVTT
                     TIEVKDAVGGVRTWVPVPLTNATDYFKREPDTWTGNFKAARAVQYDKYGTGMVFAEWA
                     PGEKAPALEVKSRFMTRDRQIDVQSKPNAAVKENPAVLTYFRQPSKLIRTDGVVATTS
                     KSIVMGLKTDSDKAKAIYDWIVENTFRDAKVKGCGIGDISTMLESGYLGGKCADLNAL
                     YVGLARAAGLPARDVYGVRVAASSEFKSLGRADDITGAQHCRAEVYLNGFGWLPVDPA
                     DVRKVVLEENLPGEPLALTDPKVKRAREKLFGAWEMNWLPYNYGHDIKLPNSKLPEIA
                     FFMYPQLETANGRRDSLDPANFKYTIKAKEV"
     gene            complement(15273..>15668)
                     /locus_tag="VE170_16195"
     CDS             complement(15273..>15668)
                     /locus_tag="VE170_16195"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYJ17033.1"
                     /translation="GLLMSVLEPDEIVTGIKVPATGGDKTAYQKAAPRSSGFAVTGVA
                     VRLAVDATGTCSRAAIGITGVADKAYRPERTEQMLTGAKLDHKLIEEAAAESTRNIEV
                     IEDINGSSEYRGHLTHVYVARAVQEALSR"
BASE COUNT         3606 a         4229 c         4359 g         3474 t
ORIGIN      
        1 ttgccctttt tgagggcaat ttcgtgatag cggatcaaca cggaattcat gcaggagaac
       61 taacagattc atgcgccttt tgcatgccgc cggtgctatg gtagtatcgg ccgacgaagt
      121 aatcaggagc gagccatgag caacttcaag aagctaaccg atgccacaag cttttcggcg
      181 gaaaagatga aaaagaatgg cgtcttcgag accgagcgat tcttctgcga cacctactgt
      241 ttcgagcccg gacaggagca gaagccgcat acccatgcca atgaagacaa ggtctactac
      301 gtgctcgaag ggcgcggttc gtttaccgtc ggcagtgaag aacgcgagat gggcgcgggg
      361 gagatcgccc tggcgccggc gggtcagagt catggtgtcg ccaatcgcag ccagtcgcgc
      421 ctagtgaccc tggtcttcgt cactcccaaa ccgcatcatt aaataagaac caatgacacc
      481 tgaacaagaa gcggtggcgc aagccaatca gaatttttat cgcgccttcg agactctcaa
      541 gatcgaagcc atggaaaacg tctgggctaa agacggcgaa attcaatgcg gtcatccggg
      601 ctggcgcatc ctgcgcggct ggggaccggt aatggaaagt tggaaaagaa tttttgccaa
      661 taccccgtcc atgcagttca ctttgaccga cgtgaaaatc gaagtgcgcg gcgagcttgc
      721 ctgggtgacg cagtatgaaa acttgaatag ctcgattgaa ggacagacgt tctccgccgc
      781 gatcctgaca atcaatatgt ttcaaaagac ggctgaaggc tggcgcatga tcctgcatca
      841 cggctcctcg gtgtcccaac cgccggatca ggatgacagc aataccgtcc actagaattc
      901 tacctcggat ccaactgcgg taataattag atatgcgagt atgggcccgg cggattgtgg
      961 gagttctctt catcttgatg cttttggtag ctttcatgtt ctcctatgcg agtctctacg
     1021 acttgctcgg caagatcgcg acaatttcat tgtggctcgt gctcatcttc gcaagcgaaa
     1081 tgttttggaa ttacctcacc gatgaataga tgctaagcga accaacttga catccacgcc
     1141 tctcgaagaa aaaactgctc tggtcaccgg ggcgggccga gggatcggcc gcgccatcgc
     1201 cgtcgcactg gccaaagccg gctgcacggt cgtcatcacc gcccggacgg cgccagagct
     1261 ggccgccgtg cggcaagaaa ttgttgcggc aaaaggcaaa gcttcaatcg tacaagccga
     1321 ccttacgcgc gacgacgaac tgcacgcgct aattgaaacc tgcggaccgg tggactttct
     1381 tatcaacaac gccggctggg gcaagcgcgc cactgtcgtg cgctcacgcg ttgacgattg
     1441 ggatcaaacc tttcgcgtca atttgcgggc gccgatgatc ctcgccaaag agctattgcc
     1501 ggcaatgatt cagaaaggcg agggcgccgt catcaacatc ggttcggtgt caggtaaaag
     1561 cggcgaagcc aacggcgccg cttactcagc ctcgaagttc ggcctgatcg gcttcactca
     1621 atcgctctat gaagaagtca gagagcaggg catcaaggtc gcggtgatct tgccggggtt
     1681 tgtcgacacg ccaatgattc cgccggtgaa acatttagat cgcagcaaaa tgatccaggc
     1741 gaatgacgtg gctcaagcag ttctttacat tctcaacgcg ccgccaacgg catgtcccgt
     1801 ggaaatcacg atacgaccgc agcgcacgcc gtacaggtag tgcgagacgt tttgccccaa
     1861 gttcccttcg aattcctgaa atttacgatt aaaagcgact aaatttccca ggttttcttt
     1921 tgctattgca ctcggctaca atccccatca gaccacggag agaaacacgg tgaagtgaaa
     1981 agcggggaga ctgactgtag tgttatgaat ctgccgccgg aacaagaagc gatacgagcg
     2041 aaatgttttc atccctcggg gaggttcgtt gaatttcccg aagaggatgt cgagacatcg
     2101 attcccgagc gattcgaaaa gatcgctgca cgatatcctg atcgggttgc cgtcaagctg
     2161 agaaatcgac tcctgaccta ccgagagttg aacgataccg cgaatcgctt ggcacgcgcg
     2221 attctggcac gacgtggaaa tacccaagag ccgatcgcgt tgctgatgga gcatgacatt
     2281 ccattgtttg tcgccatgct gggcgttttg aaaaccggca agatttgcgt ggttctggat
     2341 ccgtcgttcc cgaacgacag aaatgtcttt ctgatagaag actcgcaagc aggtcttctc
     2401 atctccgatc gcgagaatct gtcttctgcg gcagaataca cccatggcag gtgtggatgt
     2461 gtaaacatcg atgagttgga ctcgagttta tccatcgaaa acctgcggtt gccaatcgca
     2521 ccgatgaatt ttgcgtttct catttacaca tcgggctcaa ccggacagcc caaaggggtc
     2581 attcagaatc atcgaaatct actgcacgat tgcttgctct attgcaatgg cctacatgtt
     2641 tgcgctgacg atcgtatagc gcttctctac tcttgcagcg cgagccaggg cttgaagatt
     2701 acgtttgccg gattactcaa tggtgcggcg ctctatccgt tcaatatgcg acaaagaggg
     2761 gtggccgatt tacctctgtg gctcaatcag gaagaaattt cgatatattt ttcagtaccc
     2821 gtcgtttttc gccaatttac cagtaccctc accggacact accagtggcc gagtttgcgg
     2881 attatccagt tgggctcgga tttggtcacc ctcagagaac tcgaagaatt ccaaaagcac
     2941 tttactgcgg acactattct gattatcaga tttggtgcga ccgagactgg cactctgcga
     3001 cgattgtacg tcgacgcgaa aagctccctg gatcaaatca agaatgccgt ggggtatgca
     3061 accgaagaag cggaaatttc tatacgcgac gaggcagatg gacagatgaa atgtgacgtg
     3121 attggagaaa tcgtggtcca cagccgctac atctcgccgg gatattggcg acgcccaaat
     3181 ctccatcagg atccgttttt ccccgaccct aacggtcgcg gcaagcgaat ctatcgtacc
     3241 ggggatttgg gccggttacg ctctgacggg ctgctctttc atttggggcg aaaggacttt
     3301 cagttaagca tcagagggta cagggtagaa cccagcgaga tcgaagcggt attgttagcg
     3361 caggataacg tcaaggaagc actcgtcgcg gcgggcccag gggacacgca agcggagcac
     3421 gatcgtctgg tcgcttacat tgttccttat tataagcctt ttccttcgag tcccgtgctc
     3481 cgacgagccg ccaggcagaa gctgccggcc cacatgatac cgtcggactt cgtatttttg
     3541 gaatcgctcc cgcttacgcc caacggcaag gtcgatcggc tggcgctccc ggcgcccgat
     3601 acggcgcgca gcgaaatcga gatgacctat gctgctcccc ggtctgatat cgaaaatcgc
     3661 ctggtaaaaa tctggaccga agtactttcc attgagcatg tcggcattca cgataatttt
     3721 ttcgacttgg gaggtcactc gttatcagcg actcgtgtac tgtccagaat tttcgagcag
     3781 tatcagttgg agataccatt gaaatcgctg tttcagtctc caacgattgc cgaactggca
     3841 gcacaaatca ttgcccatca aaaaaacggc tagtcgagag attttggaga gtcgacgacg
     3901 aaccgcgcca tacacgaagc tgcatttata tccgtagatc ggacagctcc ctctatgcgc
     3961 aggaacagat caaactgatt ttcaaaaaat catctctgca gcgttatgcc gccggatacc
     4021 gcttacgatt tgacagcaaa aacgagaaac tgtaacccta taagaaagaa attctaaagt
     4081 tggtggtgga ggaattccca tgtccatgat cgatgccgat acgcatgtcg acgagtgcga
     4141 agaaacttgg aagaagttgg agggtacgca atacgccaag tacattccgg tgacgacgac
     4201 gatgccgccc gaggaggcca agcgggctgg ctataacccg accaacagcc gctgctgggt
     4261 ggtcgagggc cgtcttcaaa accgggcgat ccgggatgat atcaatcatc ctccacgagt
     4321 ctttcgcgaa ctccaggacg tatccggccg agtggctcac atggacaaaa tgggtgtcgc
     4381 ggtgcaggtc atttttccga ccttcttcat ccgctataac accagcaatg ccgaagctga
     4441 atgggcgttg accacaacct acaaccgctg gattgccggg aaatgcgcac aaactaacgg
     4501 ccggctgcgt tgggctgcgg tcttgccgtt cctgagtccc gacaaagcgg ttgaagaggt
     4561 gcgttgggcg aaagagcatg gcgcttgcgg tattttcaag cgcggcttcg atctcgaccg
     4621 ctatgtcacc gatccgcact tctttccggc ctatgaagag gccagcgccc atgatttgcc
     4681 actgtgcatt cacaccggcc atccgctccc gggtcacgag tgggaccgtg gctttccgat
     4741 catgtactcc tgcggtgcca tagtcagctc aggaatcgcc cagaagtttc ctaaactgag
     4801 atttggcttc attgagtcgg gggcgtcgtg gattccctat acgatttctc agctcggagc
     4861 ggttaaacgg cagactctgc gcggtcaaaa cacacgcctg cccaatcttt atgaactgga
     4921 accggagatg ttccgcgcca accggctgtt cgtaaccatc gacccgatcg acgacatcga
     4981 gtcgattctg aagttcggca cggaagataa cattatgatc ggcaccgact acagccacac
     5041 cgacatttcc gccaacctga gcgcgttgga cgaggtacgc agctgggtca aggagggccg
     5101 gatcaacgac gctcaggcgc acaaaattct tgagagcaac tccaaagaat tctatcggct
     5161 gtaacgagtt cggctgccta gcttaggtgt ggccgcaaac gatgccggag gcggattttt
     5221 agacgtgtgc gccgttgcat tattggcgaa tactttccta aagagtacct gctgcgcgcc
     5281 gatagttgct taaaggccgg ttcgcagcgc catgacgaag aaacaccttc ccaactacta
     5341 catcattctc cgggtgcagc ccaacgcgtc tgtcgatacg atcaaggcga gctttcgcaa
     5401 gctgatggtg acgatgaaga tgcacccgga cctgggtggc gatcacgatg tcgcggccca
     5461 gatcaatgaa gcctacgcgg tgctcaagga tcattcgcga cgggtcaagt acgatcgcct
     5521 gtatttgctg caacggctag aggcggccca ggccgccgcc cacgagcggg aaaaacgagc
     5581 cgcgcagggc gctggagcac ggccggctgc cggctcgaca aagcggccga gttcctcgcc
     5641 acaaacgcgc gatggcaaag ccggtcagtg cccctactgc aataccgcgc tgccgaagtt
     5701 aatcgagtcg gatacgcgct gcgagcgctg ccacagtcca ttgtctgccc cgccgcacct
     5761 aagctcgttc ggtaaggagc tgttcggccg acgcgccacg ccgcgcatag tgaagagcca
     5821 cttcgccacc gttcatccaa ccgggcaggc gcaaagcatg acggttaaga tgcgtgatct
     5881 ttctttggcg ggaataagtt tctattcgga aattaacctc gatattgaac aagtttttaa
     5941 gtttcgtgat gcatctttgg aagccatcgc agtagtggtg tcgcgcggca agcgcggtca
     6001 gtggtattcc atccatgcgc gactgctcac ggttgccttt caccagccgg gcgtctttgt
     6061 atccgccagt cgatagcact tgtttgcaag ttttcttcat gccagtaaaa accggcattc
     6121 atctttgtaa tgctgcgaag ccttccgctg acttggtgcc aaaaatctaa tcgccgaaag
     6181 caagcttgct gcactcgtgc aggctgcaga gatattaaag aatcgctagg ctgggcgccg
     6241 acaacggctg ggctcacgat aaagtgcgtc cggttgactc gcaatgagac accggcctta
     6301 tcaactccat acgttgtccg acggccaaag atcactgtta ccggcaaagt ccggcgagat
     6361 catcgagggt gacggtttcc agggttgtga cgacggcatc ggcatcgatt aaaagttcgg
     6421 ctgggtgcga gttggtaacc gccagacact tcatgcccgc gtgccgggcg cctttgatgc
     6481 cttctttgga atcttcaatg acgacacaat caccggcttg aatggcatcg ctggtgtcga
     6541 cgttaagggc tgccagcgcc tgcaaaaaaa tatccggctc gggtttgcct cgtttgacat
     6601 cttcggcgct gacgacagct ttaaagtgat ggattaagtt tgccgacgta aggatggttt
     6661 cgatctcatt gcgcagggcg cccgaggcga cggccagcgg cagactttgc gccaattcga
     6721 cgaccaactt cttgactccg ggaaatatcg ctacatggtt gcggatcgct tcctgataat
     6781 agacggcttt gcgctcgatg agttgcgcca gcttgttttg gctcaatggt cggttgtgct
     6841 cgtggtaacc gaagctgaag gcgccgcggt cgtcgaagcc cagatagcgg gcgtagtagg
     6901 cgtcgttagt cagctctatg ccttcttcgg caaggacctt ttggaagagt tcgaaatgaa
     6961 tcggctcgtc gtcgacgatg atgccgttaa aatcgaaaat gacggcgcgc agcatgcgct
     7021 taacttacag gattcgtctt ttgtttcaac gcgatctgcg ctagataagc atccatggca
     7081 gcagatcatc atcaggccca tcacgccgcc catgggcatg gccatatcga ctaccgattt
     7141 tgtccgaagt gtggcggcgg attagatagg aaaaccgtca aagcgagcga gcccgcacgg
     7201 ctggtttgcc aagcctgttc gttcattttc taccaggacc ccaaagtcgt cgccggcaca
     7261 atattcacat tgaacggtgg gatcgttctg ctccaacgcg gcgtcgaacc ggcactgggt
     7321 aagtgggtat ttcccggcgg ttacgtcgac cgcggcgaga gcgttcaaga cgctgcggta
     7381 cgtgaaacta gagaagagtg cctgctcgac gtcaagctcg gtccgctgct taatgtttat
     7441 tcgtatccgc gttcgcccaa cgtgattgta gtttataccg ctgaagtggt cggcggcacg
     7501 ttgaccgccg ccgatgaatc gacggacgcc gcagttttcg cccccacggc agtgccgtgg
     7561 catgagctcg ccttcgacag caccagggat gcgctcaacg actacattcg caattacctg
     7621 acataacgtc tatggagata tcccgctcga tccgctattc aaaatggaaa agcggaatag
     7681 attccgcttt aaatgttcgc ttgaagaaaa acttctgatt tgctctgaac cacacaacaa
     7741 aaggcccgcg agcttgaatt aagctgcggg cctcattgcc gtatgcggca tcgagctaac
     7801 ctaaatcgac caacacgtca tcgccttcga cttggacctg atacttttcc gtgggcagag
     7861 cggggagcaa ctcgttttcg ccggtggtca cattgtagcg ccagccatgc cagggacaga
     7921 tcacggtggt gccgtccatc tcgccttcgg cgagcgggcc gccgcgatgg ccacagacat
     7981 cgttgatcgc gtagaacgtg ccgtcgacat tgaagacgcc gacggccttg ccgtcgatct
     8041 ctatgctttt gccgcaaccg cctttgacat caccggtttt gcagactttt acgaaattgg
     8101 ccattaccca agttcctttc tgatcgggaa cgaatttccg cctaatatac ggagttgaga
     8161 cgtggattca agggccgggc tgggcaggag cgacgatcgg tctagcagtg accgtagcac
     8221 caaaatcacc gccgtcgtgg tgtactgtca acttggtgcc cggtgtccag cagtctctat
     8281 tgacgtagcc caaggcaatc ggctggtgaa gtgcgatcga ggcaaccgag ctcgtgatct
     8341 gacccacttc tttagcgccg gcgctgagct tgtccccagg gcttgccggc gtgtcaccat
     8401 cgagcaagag cccacaaagc ttcttgttga catggccgcg cgagcggatg cgctcgacga
     8461 cttcttggcc caggtaacag cccttggtga aactcacggc atcgtctaaa gccacttcaa
     8521 gcagcaagtt atcttcggaa aaatcgacac cgtagcgagg tataccggct tctacgcgca
     8581 ggatattttg cgcccattca ccgacccacg cggcgcctaa tgcgttcaat cgttgggcaa
     8641 agaccaacag atgagaagtt tggacgatca gatcgaagcc ctggtaacct gtgcggtcgg
     8701 cgcggacaac gcagaccggc gtgtcgtcga actgcaccat gccatgttgg ttcgcctgcg
     8761 ccggcatttc ggcattggca aacagctggc ggagcattgc ctgcgcccgt ggtccctgca
     8821 gcgagagcat tttccattgc tcgttgggat catggatctc tacctcgtcg gcaaccagaa
     8881 aacgattgag atgagcgaga atctgttctt ttagcggctc ccaaaagtcc aagtacaaag
     8941 agttcaacga acacaagacg cgcacgtccg cgaccacctt gccttgttgg gtaagaatcg
     9001 ccgcctgctg cccgtcgaat actttgagcg gcataacatc gttggaaagc atgccttgaa
     9061 ggtagggtag ccggtccgcg ccggtgaact gcagcatggc gcgattggcg aggtcgaaca
     9121 agccggcgga ttgacgaacc gatccgtact cgctcggcgg atcgccgaag tgatccggca
     9181 acagccagcc atccttttcg acgaatgtcg cccctcggcg ttcatgcaga tcgtttaatg
     9241 tcgatcgctt tgccattacc gcgaaaatat accgtcaagc gtctgcgttg acaatgccaa
     9301 cgactggttc ttaatcattg atagatagaa tagcggtgtg ataaacagaa cctatcttat
     9361 tacggaggtg cttcatggca ggacttgaag ggcaaaaggc gccggcgttt acgctcgcgg
     9421 ggagcgacgg caaacaacac tcgctggctg aatatgccgg caagaacgtt attctgtggt
     9481 tttatccgaa agacgacaca cctggctgaa ccaaagaagc gtgcgggttc cgcgacgcta
     9541 tagacaccgt gacgaaagcc aataccgtgg tggtcggtat cagcccggat ggcatcgatt
     9601 cgcacaatca attcattaca aaattcaacc tgccgttcgt gcttttagca gaccagcaga
     9661 aagaaaccat gaaagcgtat ggcgcctggg gcccgcgcaa gaatgccgcg ggtgaagtct
     9721 cggaagggac gatccgctcg accgcgttga tcggccccga tggtacggtg aagaagcatt
     9781 ggattccggt cactgatccc ggtgcccatc cggcggatgt cttggagttc ctaaaataat
     9841 tccggtaaac gtcactgagg gagcgcgggc gatttcatca accgcgctcc ctcggttgct
     9901 cgtcgcgttc aatggcaaaa atcaaagccg acctcgatct tcacaaatta gaaatattct
     9961 actgggtcgc cgaacataag agtttttccc aagctgccga gctgctctcg ctgcgccaac
    10021 cgaccatcag tgctcacgtc caagaactcg agaccgcggt cggcggcaaa ctgctctatc
    10081 gcatgccggg gaagatcagt ctgacgccgt tgggattgat gctcgccgaa cgggcgaaga
    10141 ctctactcgc cttcaagcgt gaaactgtcg ccgcggtgga gcagtttcat ggcactctca
    10201 gcggtgagct ttgggtcggc ggcagcagca ttccaggcga atatctcctg ccgcaaaagt
    10261 taggcgagtt cataaaaaaa tttccccagg tgaaaccgat cctgcgcatc ggcgactctg
    10321 ccggcgtggt ggcggatctc ctggatggca aggtcgagat cgccttcgtc ggattcaagg
    10381 gcgacgatcc gagactcaat ttcgagaaga tttgggacga cgagatggtc gttgcagtgc
    10441 ccaaggatca tccatggacc cgcggtaaag cgattcaaat cgccgatctg cgcagtgaac
    10501 gcttcatatc acgcgaacat ggctcgggga cgttggagtc catccagcaa ctgctcggca
    10561 agcgcaaacc gccgggtcgc gggcttttga atatcaccat ggaactcggc agcacggaag
    10621 ccgtgaagga agccttgatg gccggctacg gcttctcgat tttatctcgc atctcgattc
    10681 gccacgagct cgacgaaggc agcatcgtcg aagtgccggt ccgcggattg aaactcgaca
    10741 gaggttttta ccaagttttt cacagtcgcc ggccgttcca tcccatcgct caagcgtttc
    10801 gcgctttcct caaactgccc taattcatcg attttgttcg ttcgaaaagt ttttccgcgt
    10861 ggtcttccat agccaatacc tattttgtat atagatataa tgaattggga gaggagttaa
    10921 tcatggcact gcccttaatg agccgcggtc gacgcgcggc cgaggagtta cgagaaagat
    10981 tgatcgacgc ctgggcgcgc tcggacaaga tatttgccat tgtcggcagc ggccagactc
    11041 tgcaccagcc gattgtttgg cgccacccat ttatatttta tatcggccac ctgccggcgt
    11101 tttcttggaa ccaaatttgt ggcgggattc tgcaatggtc gtctgtcaat gcatattacg
    11161 acgatctgtt ctgccgcggc atcgatcccg atgtcgacac cggcgagtgc cattggcacc
    11221 cggaagtacc ggaagattgg ccaagtctcg gcgacacgct tgaatatcgc gacaaggtgc
    11281 ggcgcgccct cctggaatcg acaattgcgc tcggctatag ccgttccaac gatctgttgg
    11341 ctcaaggtgg gcgcgtcttc gagttgatct tggagcatga attcatgcat caagagacgc
    11401 tgctctacat gatgcaacag atggcacccg gtgataagat ccgcccacat gacttgccac
    11461 gttattcctt cggccgggcc acgagagcgg aaagcattcg tataccggcc ggcaaggcgc
    11521 ggctaggcgc acatttcgag gacctcagct ttggctggga taacgaattt tccgaggtca
    11581 ccgtcgacgt cgaatcgttt gccatggaca gtttgccggt caccaacggc gcattcttcg
    11641 aattcctgta tgccggcggc tacgacgagc cacgctattg gcggccggaa gactggcgct
    11701 ggcggcagct tgagcgtaag caatctccga cctgctggct cgaacaagaa ggctcctggt
    11761 ggtacaaggc gatgttcgac ctattacctc tggaacaagt ggcaagctgg ccggtgtctg
    11821 ttagtctggc ggaggcgcgc gcctatgcgg cgtggagggg taaacgttta ccgacggaaa
    11881 gcgagtatca tcgagccgct ttttacggtc cggaaggggt ggagtcaatc tatccatggg
    11941 gtgaagccgg tcccacaccg cgccacggca atttcgactt taccgcgtgg tcgccgcagc
    12001 cggtcggttc acatcccgcc ggtgctagcc gttggggcgt tcaagagctg gtcgggaatg
    12061 gttgggagtt gattgataca ccgtttgcac cactgccggg attcacaccc tacatgacaa
    12121 cctatccgga ctattccaag gatttttttg acggcaaaca ttttgttgtc aaaggtgcgt
    12181 cgtgggccac ggatgcggga ctggtgcgtt cgagctttcg caattggtat caagcgcact
    12241 atccctacgt gttcgccaag tttcgctgtg tcactgactg accgggctga atcgaaagga
    12301 ctaaccatga aagccgtttg ggagaacaca gtcgtcgccg aatcgaacca gaccattgtc
    12361 atcgaaggca atcattactt cccgccggat tcgatcaaga aagaatgctt cgcgccgagc
    12421 gacacccaca gcacctgccc ctggaaaggc ttagccagtt actatcatgt gaaagcaggc
    12481 gagaagctca accaagatgc cgcatggttt tatccggagc ccaaagatgc ggcgaagtcg
    12541 atcaaaaact acgtcgcatt ttggcgtggc gtgaccgtga gcgagtaatc gacatctgac
    12601 tattgtgaga actttgcgca tcgcctcgtg catggcggag aacaccgaag atttctgccg
    12661 ggctatcgtc gagtatctcc aactgaagct cgatgtcccg gcggagtatg tgaccggcat
    12721 tccttggcag gagcgtgagg tgcttttcga tcgcggcgag attcaagtgc tttggctctg
    12781 tggattaccg tacgttcaca aagccgacct tgccgagagc ggcatggagt tgctcgccgt
    12841 gccgataccg gtgggagagc gctacggatc gcggccggtt tacttttccg acatcgtcgt
    12901 gcgccgggat agtccttgcc gatctttccg cgacttacgc ggcgcgacct gggcttataa
    12961 tgagccgcgt tcccattccg gtttgaatgt gcgcgcgcat ctgttcgagc ttggtgaaat
    13021 cgatagtttt tttgccgccg cggtcgaagc cggcgcccat tccaattcat tgcaaatggt
    13081 cctatccgga gtcgtcgacg gcgcggccat cgacagcacg gtgtgttaga gtggctcggc
    13141 agccagcggc atgatttagc agatgagatt cgcgtcatag acaccctggg gccgagcccg
    13201 attccgccct gggtgatctc acgtcatgtt gcggcatccc aacggggcgc gctgcgccag
    13261 ttgctcgccg aattgcatct cgatccaatt ggccgcgata tcctcgcgga tggccgattg
    13321 ttgcggttta ccgcggccga tgaccgcgat tacgatccga tccgagcgat ggcgcataag
    13381 gcggatcggg tttcgttagt ttgatcgcct gtgctaaata atccaacgta tcgaaaaacc
    13441 gttcaattaa ctttatgagg agttgactca tggctagttt agaagggaaa aaagctccgc
    13501 ccttcagtct gcaagggagc gacggcaaga agcattcgtt aacagactac cagggcaaga
    13561 cggtgattct gtatttctat cccaaggaca atacccccgg ttgcaccaaa gaagcctgcg
    13621 gctttcgcga cctaaatgtc tcattgaaga aacccggaac cgtggttctc ggtgtcagta
    13681 aggatagcat cgagtcccac ggaaaattcg gcgcgcttta caaattgcca ttcacgttgc
    13741 tgtcggatcc caatgccgag gtgatgaaaa aatacggtgc cttcggcaag aagatgatgt
    13801 acggcaaaga ggtccagggc acgattcgct caaccgtagt gatcgatccc aagggtgagg
    13861 tgatcaagca ttggacgacg gtgaagaagg cagaggagca tcctcaggca gtatttgatt
    13921 ttctaaataa taaataaagc tggttctggc aaaaccgtcc ttctcgcttg ctgaattgac
    13981 aacccgccgc taaggggtat gatcaacgcc atcgaaataa cctattcttt aaatcgggag
    14041 gaactatgga tcgccgtgag tttttgaaac aagggttgac agcctctgcg ggcttagcct
    14101 tggtcggttt gccgaagttc accgggtggt cacatgcgga tgacccgacc aagtggcgca
    14161 gtttcgaagt aacgacgacg atcgaagtga aggatgcggt gggcggcgtg cgcacatggg
    14221 tgcccgtgcc cttaactaac gctacagatt attttaagcg tgaacccgat acctggacgg
    14281 gcaatttcaa agcagcccgc gcggtgcagt acgacaagta cggcaccggc atggttttcg
    14341 cggaatgggc tccgggcgaa aaggcgccgg ctcttgaagt caaaagccgc ttcatgaccc
    14401 gcgatcgtca gatcgatgtg caaagcaagc ccaatgccgc ggtcaaagag aatccggcgg
    14461 tgctgactta ttttcgccaa ccgtccaagt taatccgcac tgatggcgtc gtggcgacga
    14521 cctccaagag catcgtgatg ggattgaaaa ccgatagcga taaagcgaaa gcgatttacg
    14581 actggattgt cgaaaatacc ttccgcgatg ccaaggtcaa gggctgcggc atcggcgata
    14641 tctcgaccat gctcgaaagc ggctatttag gcggcaaatg cgccgatctc aatgccctgt
    14701 acgtcggtct cgctcgcgcc gcgggattac ccgctcgcga tgtctacggt gtgcgtgtcg
    14761 ccgcttcgtc ggaattcaag agtctcggac gggccgacga catcaccggc gcccagcatt
    14821 gccgcgcgga agtctatctc aatggctttg gttggctgcc agtagatcct gccgacgtgc
    14881 gcaaggttgt ccttgaagag aatttgcctg gagaaccact ggcactgacc gacccaaagg
    14941 tgaaacgggc gcgcgaaaaa ctattcggcg cttgggaaat gaactggcta ccctacaact
    15001 atggccacga tatcaagttg ccaaactcca aattgccgga aatcgctttc ttcatgtacc
    15061 ctcaattgga aaccgccaac ggccgccgcg atagcttgga cccggcaaat ttcaaataca
    15121 cgatcaaggc aaaagaggtc tagagcgaat tttgcacgat gattcacgca aggcctccac
    15181 aatctggggg ccttgttatt ttggcggtgg aaaacaacaa gagtttgggc gcggcatgct
    15241 gcgctccaaa cgcaaaacct acggtagtgg gactagcggc ttaatgcttc ttgtaccgca
    15301 cgggcgacgt agacgtgggt cagatggcca cggtactccg acgagccgtt gatgtcttcg
    15361 atgacctcga tattgcgggt cgactcagca gccgcttcct cgatcaattt atggtcgagt
    15421 ttggcgccag ttagcatttg ctcggtgcgc tcgggccgat aggctttgtc tgccacgccg
    15481 gtgatgccga tggcggcgcg gctgcaagtg ccggtggcat caaccgccaa acgtactgcc
    15541 acacccgtga cggcgaagcc ggaagagcgc ggcgcggctt tctgataggc ggtcttgtcg
    15601 ccgccggttg ccggcacttt aattccagtg acgatctcgt ccggttccag cacactcatc
    15661 aataggcc
//
LOCUS       DASQKS010000625         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4962022, whole genome shotgun sequence.
ACCESSION   DASQKS010000625 DASQKS010000000
VERSION     DASQKS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4962022"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VE170_16200"
     CDS             complement(<1..271)
                     /locus_tag="VE170_16200"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ17034.1"
                     /translation="MDDELTAPLDTATTANPARARLRTFSSLRHADFRYLWTGTVMMS
                     AGQWVQQVTLGWLLYDLTGNSVLLGALNGLRALPFLVTGPMAGVAA"
     gene            complement(473..1513)
                     /locus_tag="VE170_16205"
     CDS             complement(473..1513)
                     /locus_tag="VE170_16205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17035.1"
                     /translation="MDEVEVIDADGHVLETDAELEEYFDGEYTGHRRSGVFSIFPSLD
                     GWPRGFVRGLNKVTRISAKEWIEFIDGSQISAAVLYPTAGLSFGLIQDPDWACVVARM
                     YNNWLADRYCKVDPRLKGVAMLPVHNPQEAADELHRAVKELGMVAGLLPAVTMLNKGY
                     GHEDFHPIYKQAEALNVPLTVHGAVSRGLGFDFLQTMSMIHTLEHPLAQMIQLTSMVL
                     DGVFDLFPKLRVGFLEAGAGWIPYMMDRMDEKFHIDRKRKHFPLSVKPSEYFKRGNIY
                     VTCETDEKTLDAVVRELGENHVMYPTDFPHERQAGEFAVDIPEFWQRRDLSEPAKRKI
                     LSENAKRFYALP"
     gene            1767..>2317
                     /locus_tag="VE170_16210"
     CDS             1767..>2317
                     /locus_tag="VE170_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17036.1"
                     /translation="MIDGYRIYDADAHVNVAPQMWEDLPKEFAARRPRPALVHDGAEL
                     AAFTAGWLTEGRMEPHALGPGLQPANPPRWVVEGTDRGSLTLVDADARIRDLDRLGID
                     VQFLFPSTLYARWSADPRLEAALFRAFNRFIARQTKPYAKRLKWAGLIPLRHPRESIA
                     ALEEMDKLGASAAVVFGTARDKM"
BASE COUNT          448 a          640 c          717 g          512 t
ORIGIN      
        1 cggcggcaac ccccgccatc ggtccggtga ctaggaacgg cagcgcgcgc agaccgttga
       61 gtgcaccgag aagcaccgaa ttgccggtga gatcgtagag cagccagccc aatgtgactt
      121 gctgcaccca ttgaccggcg ctcatcatca ccgtgccggt ccagagataa cgaaagtcgg
      181 cgtggcgcag cgacgaaaag gtgcgcaagc gagcgcgtgc gggatttgcg gtggttgcgg
      241 tgtcgagcgg cgctgttagt tcatcgtcca agtgtctaat ttccaaaacc gtatagtatc
      301 atgaatttta acaagttcac ttcgttcgag aaaatcttct gcgctatccc gacccttgcg
      361 gcctcggcat cgctcggcct gtgccgaata catcgggttc gggagccctc gcggcgcgcg
      421 gttgtggcga tgccgctgca ctgggttctg tatcgccttg gcggcgattg tttcatggca
      481 gcgcgtagaa tcgcttggcg ttctcactga gaatcttacg cttggccggc tcggataaat
      541 cccggcgttg ccagaactcg gggatgtcga cggcaaattc gccggcttgg cgttcgtgcg
      601 ggaagtcggt ggggtacatc acatgatttt ctccgagctc gcggacgacg gcgtcgaggg
      661 tcttttcgtc ggtctcgcag gtgacataaa tgttgccgcg cttgaaatac tcgcttggtt
      721 taacgctcag cgggaaatgt ttgcgcttgc ggtcgatatg aaatttttcg tccatgcggt
      781 ccatcatgta gggaatccag ccggcgcccg cttcgagaaa gccgactctt agcttgggaa
      841 ataaatcgaa cacgccgtcc agcaccatgc tggtgagttg aatcatctgg gcgagcggat
      901 gttcgagggt gtggatcatc gacatggtct gtaaaaaatc gaagcccaag ccgcggctta
      961 ccgcgccatg cacggtgagc ggcacgttga gcgcttcggc ttgcttgtag attggatgga
     1021 aatcttcatg gccgtagcct ttgttgagca tcgtcactgc cggtaatagg ccggcgacca
     1081 tgcccaactc tttaacggcg cggtggagtt cgtcggctgc ctcttgcggg ttgtgcaccg
     1141 gcagcatggc gacgcctttt agacgcggat cgactttgca gtagcgatcg gcgagccagt
     1201 tgttgtacat gcgcgcgacc acgcaggccc agtcgggatc ttggataaga ccgaaggaga
     1261 ggccggcggt gggatagagc acggcggcgc tgatctgcga accgtcgatg aactcgatcc
     1321 actccttggc ggagatgcgc gtgactttat tcaaaccccg gacgaagccg cgcggccaac
     1381 cgtcgagaga cggaaagatt gaaaaaactc cggagcgccg gtggccggtg tattcaccgt
     1441 cgaaatattc ttccagttct gcgtcggtct ccaagacgtg accgtctgcg tctatgactt
     1501 ccacttcgtc catcgcgagc gttctcccgg caaaaagatc tgcagagctt ggcttagcat
     1561 ccgggtgcaa tttttgtcaa cgcagcgtca tacggcaaat ccgtcatttt cggaaaacgc
     1621 aatctagatt ttcgtctggt gcataaacct ggaatgtcgc gtttgcgaaa atgacgctag
     1681 ggcaatcgcg gtgcgccgtt ttggcgttga cagcgctcgg aagcgcgtgc tttaacgggc
     1741 taaatcgaat acctgggaga atgccgatga tcgacggtta tcgaatctat gacgctgacg
     1801 cccacgtcaa tgtcgcgccg cagatgtggg aagatctgcc caaggagttc gcggcgcgcc
     1861 ggccgcgccc ggcgctggtt cacgatggcg cggagctggc ggctttcacc gccggatggt
     1921 tgaccgaggg gcgcatggag ccccatgcgc tgggcccggg attgcaaccg gccaacccgc
     1981 cgcgttgggt ggtcgaaggc acggatcgcg gttcgcttac tctggtcgat gccgatgcgc
     2041 gcattcgcga tctcgaccgc ttgggcattg acgtgcaatt tctctttccg tccacgcttt
     2101 acgcccgctg gagcgccgat ccgcgattgg aagcggcgtt gtttcgcgct ttcaatcgtt
     2161 tcatcgcacg ccaaaccaaa ccctacgcca agcgattgaa atgggccggg ttgattcctt
     2221 tacgtcatcc gcgtgagtcg atcgccgcgc tggaggaaat ggataaactc ggcgcctcgg
     2281 cggcggtggt gtttggcacc gcccgcgaca agatgtt
//
LOCUS       DASQKS010000626         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3302753, whole genome shotgun sequence.
ACCESSION   DASQKS010000626 DASQKS010000000
VERSION     DASQKS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302753"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VE170_16215"
     CDS             <1..510
                     /locus_tag="VE170_16215"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYJ17037.1"
                     /translation="PVKPAGGAAGPAAKNDEITFYDTLAKSRDAQALAEPPAKVAKPA
                     ENNHRADVKELKAEAKPDGQLAKLNNSKTDKVQPTTESTKIETADAEAKVWRAQVDAY
                     PDERSAKLLVDRLKNKGYNAYVNEVEFRGKPWFRVSVGKYNSREEADKVVGALKDKEN
                     HAKAFAASK"
     gene            529..1545
                     /gene="trpD"
                     /locus_tag="VE170_16220"
     CDS             529..1545
                     /gene="trpD"
                     /locus_tag="VE170_16220"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYJ17038.1"
                     /translation="MNIREAIDKLVNRIDLTEAQTVDVMNQIMTGEATPLQVASFLTA
                     LRMKGETVGEITGAARVMREKAHRVNVGTKTVLDTCGTGGDQKGTFNISTTAAFVVAG
                     AGVNVAKHGNRSVSSQSGSADVLGALGVKVDAPKERVERCIAQIGIGFLFAPLLHEAM
                     KYAVQPRRDIGIRTIFNLLGPLTNPAMATHQLIGIYNGDLVGMVANVLKNLGSTRAMV
                     VHGLEGLDEISLCGPTKVAELRDGAVKEFQVEPEQFGLKRCQLGDLHGGSAEESAVSV
                     RAVLDGKPGAPRDVVLLNSGAALYVSGSAASVGDGIKLAAESIDSGKAREKLSQLAEL
                     TNAA"
     gene            1552..2328
                     /gene="trpC"
                     /locus_tag="VE170_16225"
     CDS             1552..2328
                     /gene="trpC"
                     /locus_tag="VE170_16225"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYJ17039.1"
                     /translation="MAEILSTIAAHVRLVVARRQRELPFTALRDRALYHAPTRGFATA
                     LTGNQRRIVAEIKKASPSKGLIRADFDPIAIAKDYASHGASAISVLTEERFFQGDLAY
                     LEQIHASVGVPLLRKDFMLDPYQIVEAKSFGADAVLFIAAMLDRQLMGELRQQASELQ
                     MDALVEVHSEIELEAAVNAGAQLIGINNRDLTTFAVSLATTEKLAPLVPAGLPVVCES
                     GIDSIEQIRWVERLGIHAFLIGESLMRAPAPGKKLRELLT"
     gene            2391..3017
                     /locus_tag="VE170_16230"
     CDS             2391..3017
                     /locus_tag="VE170_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HYJ17040.1"
                     /translation="MVKIKVCGITNLADAEKAIAFGADILGFNFYPSSPRSIAPAKAQ
                     EIIACLPHDSFNVALFVNAPKARVNEVLTAARLPDGRQAYRGLQFHGDEDGAYCQDWQ
                     VKVIKAFRIKAQASLAALDKFPADYYLLDSFSAGYGGSGQMFPWEWLAGIDTGRIILS
                     GGLRADNVAEAIGKIKPFAVDVCSGVEARPGVKDHGKLKEFIAAAKGA"
     gene            2974..>3884
                     /gene="trpB"
                     /locus_tag="VE170_16235"
     CDS             2974..>3884
                     /gene="trpB"
                     /locus_tag="VE170_16235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYJ17041.1"
                     /translation="MANSKNLSLLPKVPDRRGHFGPYGGRYVAETLMPALTELERGYA
                     QVRRDAGFKRELAFYLKEYVGRETPLYFAERLTRSLGGATVYLKREDLCHTGAHKINN
                     TMGQILLARHMGKRRIIAETGAGQHGVATATVAARFGMECEIFMGAEDVRRQSLNVFR
                     MKLLGAKVRVVQSGSRTLKDAMNEALRDWVTNVRNTYYLIGSVAGPHPYPMMVRDFQS
                     VIGREARRQILRKEGRLPDYLVACVGGGSNAMGLFSPFVKDRSVKMLGVEAAGHGLGS
                     GKHSATLQRGAVGVLHGSKSYLLQDDKG"
BASE COUNT          851 a         1089 c         1194 g          750 t
ORIGIN      
        1 ccagtcaaac cagccggtgg ggccgctggc ccggcggcga agaacgacga aataactttc
       61 tacgacacct tggcgaaatc ccgcgacgct caagcgctag ccgagccgcc ggccaaagtg
      121 gcgaagcccg cggagaataa ccatcgcgcc gacgttaagg agctcaaggc tgaggctaaa
      181 cccgacggcc agttggccaa gctgaacaac agtaaaaccg acaaggtcca accgacgaca
      241 gagagcacca agatcgaaac cgccgatgcc gaggctaaag tttggcgcgc gcaagtggac
      301 gcctatcccg atgagcggtc ggctaaactg ttggttgatc gactaaaaaa taaaggctac
      361 aacgcctatg ttaacgaggt tgaattccgc ggcaaaccct ggtttcgggt cagtgtcggc
      421 aagtacaact cgcgcgagga agccgacaaa gtagtgggcg ccctcaagga caaagaaaac
      481 catgcgaaag cgttcgccgc ctccaaataa gttcgacggc gattgatcat gaacatccgc
      541 gaagccatcg ataaactagt caaccgcatt gacttgacgg aagcgcaaac ggtcgatgtc
      601 atgaatcaaa tcatgaccgg tgaagcgacg ccgctgcagg tggcctcctt tctgaccgcc
      661 ctgcgtatga aaggcgaaac cgtcggcgag atcaccggcg cggccagggt gatgcgcgaa
      721 aaagcgcatc gggtcaacgt cggtactaag acggtgctcg acacctgcgg caccggcggc
      781 gaccaaaagg ggactttcaa tatttcgact accgctgcct tcgtggtcgc cggcgccggc
      841 gtcaatgtcg caaaacatgg caaccgttcg gtgtcgagcc aatcgggcag cgccgatgtc
      901 ttgggtgcat tgggcgtcaa ggttgacgcg ccaaaagaac gggtcgagcg ctgcatcgcc
      961 cagatcggga tcggtttttt gttcgcaccg ctgcttcatg aagcgatgaa gtacgctgta
     1021 cagccgcgcc gcgacatcgg cattcgtacg atattcaatc tactcggacc gctgaccaat
     1081 ccggctatgg cgacgcatca gctgattggc atctacaacg gcgatttggt cggcatggtg
     1141 gccaacgtgc tgaaaaatct cgggagtacg cgggcgatgg tcgttcacgg actcgaaggc
     1201 ctggatgaaa tttctctatg cgggccgact aaagtagcgg agctgcgcga tggggcggtg
     1261 aaagagttcc aagtcgagcc cgagcagttt ggtctcaaac gttgccaact cggcgacctc
     1321 catgggggca gcgcggagga gagcgcggtg agcgtccgcg ccgtgctcga cggcaagccc
     1381 ggcgcgccgc gcgatgtcgt gctcttgaac agcggcgcgg cgctttacgt cagcggcagc
     1441 gccgccagtg taggggacgg gatcaagctg gctgcggaat ccatcgacag cggcaaggcg
     1501 cgcgaaaaac tctcacagct ggccgaactg accaacgccg catagttttt catggctgaa
     1561 attttgtcca ctatcgcggc gcatgtgcgc cttgtggtcg cgcgtcggca gcgcgagctg
     1621 ccatttaccg cgctgcgcga tcgagcgctt tatcatgcgc cgacccgcgg cttcgccacg
     1681 gcgctcaccg gtaaccagcg gcgcattgtt gccgagatca agaaagcctc accctccaag
     1741 gggctgattc gcgcggattt cgatccgatc gcgattgcca aggattatgc gagtcatggg
     1801 gccagcgcga tttcggtctt gaccgaagag cgttttttcc aaggtgatct ggcgtatctc
     1861 gaacagattc acgcgagtgt gggggtaccg ctgttacgca aggatttcat gctcgatccc
     1921 tatcaaatcg tcgaagcgaa gagcttcggc gccgatgcgg tgctgttcat cgcggcgatg
     1981 cttgaccgcc aactgatggg cgagctgcgc cagcaggcga gcgaattgca gatggacgcg
     2041 ttggtggaag tgcatagtga aatcgagttg gaagcggccg taaacgccgg cgcgcagttg
     2101 atcggcatca acaaccgcga tctcacgact tttgccgtga gcttggcgac gacggaaaaa
     2161 ttggcgccgc tcgtgccggc gggactgccg gtggtgtgcg aaagcggcat cgatagcatc
     2221 gagcagatcc gctgggtcga aagactcggt atccacgcgt tcctgatagg cgagtcgcta
     2281 atgcgcgcgc cggcgccggg aaaaaaattg agggaactct taacctgact cgtgttcatc
     2341 ttccgtgagt aaacctagat acgattttcg agcacgaggg cgaacacgac atggtcaaga
     2401 taaaagtttg cggcattacc aatctcgccg acgccgagaa ggcgatcgcg ttcggcgcgg
     2461 atattttagg ttttaatttt tatccgtcga gcccgcgctc cattgcgccg gcaaaggcac
     2521 aggaaattat cgcttgcctg ccgcacgatt cgttcaatgt ggcgctgttt gtcaatgcgc
     2581 cgaaagcgcg ggtcaacgag gtgttgacgg cggcgcgatt gccagatggg cggcaagctt
     2641 atcgaggctt gcaatttcac ggcgacgagg acggcgccta ctgtcaagac tggcaagtga
     2701 aagtcatcaa ggcgtttcgc atcaaagcgc aagcttcgct tgccgctttg gacaagtttc
     2761 cggcggatta ttacctgctc gattcctttt ccgctgggta cggtggttcc ggacaaatgt
     2821 tcccttggga atggttggct ggcatcgaca ccggcaggat tattctttcc ggcggcctgc
     2881 gcgccgacaa tgtcgccgag gcgatcggca aaattaagcc gtttgcagtc gatgtttgta
     2941 gcggcgtgga agcgcgccca ggtgttaaag atcatggcaa actcaaagaa tttatcgctg
     3001 ctgccaaagg tgcctgaccg gcgcggccat ttcggcccct atggcggccg gtatgtcgcc
     3061 gaaaccctca tgcccgcatt gaccgagctg gagcgcggtt atgcgcaggt ccggcgtgac
     3121 gccggcttca agcgcgaact ggccttttat ttaaaagaat atgtcggccg agaaacgccg
     3181 ctttatttcg cagaaagatt gacccgcagc ctgggaggcg ccacggtcta tctcaagcgc
     3241 gaggatcttt gccacaccgg cgcgcacaag atcaataaca ccatggggca aatcctcttg
     3301 gcccggcaca tgggtaagcg gcgtatcatc gccgaaaccg gcgccggtca acatggcgta
     3361 gccaccgcca ccgtggcagc gcgcttcggt atggagtgcg agatcttcat gggtgcagaa
     3421 gacgtccggc gccagtcgct caatgttttt cgcatgaagc tcttgggcgc caaagtgcgc
     3481 gtggtccaat ccggcagccg gacgctcaaa gatgccatga acgaagcgct gcgcgactgg
     3541 gtaaccaacg tgcgcaatac ctattatctc atcggctcgg tcgccgggcc gcatccctat
     3601 ccgatgatgg tgcgggattt ccaatcggtc atcggtcgcg aggcgcgccg acaaatcctc
     3661 cgcaaagaag gccggctgcc ggattatcta gtcgcctgcg tcggcggcgg cagcaatgcc
     3721 atgggactat tttctccgtt cgtcaaagac cggtcggtca agatgctcgg cgtcgaagcc
     3781 gccggtcacg ggctgggatc cggcaaacac tcggcgaccc ttcagcgcgg cgccgtcggg
     3841 gttttgcacg gcagcaaaag ttatctgttg caggacgaca aggg
//
LOCUS       DASQKS010000627         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4153653, whole genome shotgun sequence.
ACCESSION   DASQKS010000627 DASQKS010000000
VERSION     DASQKS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4153653"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VE170_16240"
     CDS             complement(<1..874)
                     /locus_tag="VE170_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17042.1"
                     /translation="MTENLLTPYRERLKSISPYRLCGTVTEITGLLIVSRGPWLPVGG
                     VCHIYPSGGANPVLAEVVGFRDDRILLMPLGDLRGVGPGSRVVALTKEAHLPVSEKLL
                     GRVLDGLGRPIDGKGIVAAHSYPIYVANSNPLDRQEISEPLDIGIRAINGLVTLGRGQ
                     RVAIMAGAGVGKSTLLSMIARNTRADVKVIALIGERGREVEDFVSRTLPAGERERMIV
                     VAATSETPALVRVRGAFIATTIAEYFRDRGQHVLLLMDSLSRFAMAQREAGLAAGEPP
                     STKGYTPSVFALLPR"
     gene            complement(1014..1739)
                     /locus_tag="VE170_16245"
     CDS             complement(1014..1739)
                     /locus_tag="VE170_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HYJ17043.1"
                     /translation="MSRLVARGDLHAAEVCVYRQADLDGDTMTSTPADNADTDYRGFE
                     LESLDALELAPWWILQCAQEKAAEIIAQATVQAEQLRQQASCENAAAGRAQALQEVMP
                     SLVVFANAAQSLIVFEEQLISRCTPQIVGLALEIASKVIAKAVDADGEVVAAVLERAK
                     QEALDAKQLRICLNPTDHQLLAELRPDLVKIGNDGKRTIEVVASAEIARGGCRLETES
                     GIVDATLPTQIDEIRRHLLDTEV"
     gene            complement(1732..>2040)
                     /locus_tag="VE170_16250"
     CDS             complement(1732..>2040)
                     /locus_tag="VE170_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HYJ17044.1"
                     /translation="MFTFEDLGKIDDASMQVLVREIEKDKWVMAMRTASPALKKKLFA
                     NMSERAGALLKEEIENMGPVRLRDVEAVQREVIDRARQLEGEGKVFLSGGKSKEDLLV
                     "
BASE COUNT          398 a          620 c          608 g          414 t
ORIGIN      
        1 accggggcaa gagggcgaac accgagggcg tgtagccttt cgtcgacggc ggctcgccgg
       61 cggccaagcc ggcttcgcgc tgggccatgg cgaaacggct gagcgaatcc atcaacagca
      121 atacatgttg gccgcggtcg cgaaaatatt cagcaatggt ggtggcgata aatgcgccgc
      181 ggacgcgcac caacgccggc gtttcggagg tggcggcgac cacgatcatg cgctcgcgct
      241 cgccggcggg cagggtgcga ctgacaaagt cttcaacttc gcgaccgcgc tcaccgatca
      301 gcgcgatgac ttttacgtcg gcgcgggtgt tgcgcgcgat catcgagagc agcgtgctct
      361 taccgacgcc ggcgccggcc ataatcgcca cgcgctgtcc ccttcccaat gtcaccaagc
      421 cgttgattgc gcggatgccg atatcgagcg gttcgctaat ctcctggcga tcgagcggat
      481 tcgaattggc gacatagatg ggataggaat gcgccgcaac gatgcccttg ccgtcgatcg
      541 gccgtcctaa accatcgagc acccggccga gtagtttttc gctgaccggc agatgcgctt
      601 ccttcgtcaa ggcgacgacg cggctgcccg ggccgacacc gcgcaggtcg ccgagcggca
      661 ttagtaaaat gcggtcgtca cgaaatccga ccacttcggc gagtaccgga ttggcaccgc
      721 cgctcggata gatatggcac accccgccga ccggcagcca gggaccacgg ctgacaatca
      781 atagtccggt gatttcggtc accgtgccgc aaagacgata aggcgaaatc gacttcagcc
      841 gctcgcgata aggcgtcagc aaattttctg tcatagatgc gcgatccctg gttcaatgca
      901 gcgattcact gtggcgaggt tcgaatcctg tgcttgtcgt tagaaattta ttaaatcgaa
      961 gcagaaaaga ccgcgcgcat gatggattcg cccaggctta gcaagcgcgg actctacacc
     1021 tctgtatcca aaaggtgccg gcgaatctca tctatttgag tcggtagggt ggcgtcgacg
     1081 atgcctgatt cggtttccaa ccggcacccg ccgcgggcga tctctgcgct ggcgacgacc
     1141 tcgatggtgc gcttaccgtc gttgccgatt ttgactaagt ccggccggag ctcagcaagc
     1201 agctggtgat cggtcgggtt gaggcagatg cgcagctgct tggcgtcgag ggcctcttgt
     1261 ttggcgcgct cgagcacagc ggcgacgact tcgccgtcgg catccaccgc cttggcgatc
     1321 accttagaag cgatctccag cgctaagcca acgatttgcg gcgtgcagcg cgagatcaat
     1381 tgctcttcga agacaattag cgattgggcc gcgttggcaa aaaccaccag cgacggcatg
     1441 acttcctgca gcgcttgggc gcgccccgcc gcggcatttt cacacgacgc ttgctggcgc
     1501 aactgttccg cctgcacggt tgcctgggcg atgatctcgg cggctttttc ctgggcacac
     1561 tgaagaatcc accatggcgc gagttcgagg gcgtcgagag actctaactc aaagccgcga
     1621 tagtccgtgt cggcattatc ggccggcgtc gacgtcatcg tatcaccgtc cagatcggct
     1681 tggcggtaga cacaaacttc cgccgcgtgc aaatcgccgc gcgccaccag tctagacaag
     1741 cagatcctcc ttggatttgc cgccggagag aaagacttta ccctcgcctt caagttggcg
     1801 cgcgcggtcg atgacttccc gttggacagc ttcaacatcg cgcagtcgca ccggccccat
     1861 attctcgatt tcttctttga gcaaggcacc ggcgcgctcc gacatgttgg cgaagagttt
     1921 ctttttgagg gcgggactcg ccgtgcgcat ggccatgacc catttgtctt tttcgatttc
     1981 ccggaccagc acctgcatgc tggcatcgtc gattttgccc aagtcttcga aggtgaacat
//
LOCUS       DASQKS010000628         9023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3724999, whole genome shotgun sequence.
ACCESSION   DASQKS010000628 DASQKS010000000
VERSION     DASQKS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724999"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1745)
                     /gene="glgP"
                     /locus_tag="VE170_16255"
     CDS             complement(42..1745)
                     /gene="glgP"
                     /locus_tag="VE170_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYJ17045.1"
                     /translation="MFTKEILPNYVAYFSMEVGLDSSMPTYSGGLGVLAGDTLRAAAD
                     LGLPMIGVTLLHRKGYFRQSLDAGGNQTESPAGWNYEEVLEPMVPRAFVPIAGRAVMV
                     RAWRYIVRGVFGHTVPVYFLDTDLKENTPWEQSLTESLYDGDGRHRLCQEVVLGMGGV
                     AMLQALGYKNVSTHHMNEGHAAFLTLALLEREAARGDLAAVAETDCEAVRQRSVFTTH
                     TPVPAGHDQFPVALVSEVLGATRLAALEKLQCIQGGMLNMTYLALHLSRYINGVAMRH
                     GEISRDLFPDHAIDSITNGVHALTWTAAPFRNLYDRFIPEWRRDSFYLRYAIKIPLAD
                     VQRAHAHAKREMLAQIALRTKAHLSENVLTICFARRAAGYKRADLLFTDLERLEAITE
                     RSGPFQIIFSGKAHPHDETGKAMIRRIFEFSKKLKDSLRVVYLENYDMDLARSLCAGV
                     DLWLNTPERPLEASGTSGMKAALNGVPSLSVLDGWWVEGHVEGVTGWAIGDDGVADLA
                     SEADSLYDKLESAILPLYYRQPDAYAAIRRSAISLNGSYFHTQRMVLQYVRNAYLGVE
                     P"
     gene            2247..3680
                     /locus_tag="VE170_16260"
     CDS             2247..3680
                     /locus_tag="VE170_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HYJ17046.1"
                     /translation="MKFELYETTDFYDEMFVSGSAPRAGARLLKERIESLPDGELFAR
                     QAAAESLLLQNGITFNVYSDSAATEKIFPFDVIPRIVEAAEWDWIEKGLKQRVYALNL
                     FIDDIYHQQKIVKDKVVPEYLIQSARSFRKECIGLNPPQKVWCHITGTDLVRDRDGQI
                     YVLEDNLRCPSGVSYVLENRRIMKRTFPQVFEASRVRPVDEYPSRLLSTLQSLFPAAI
                     DSPKVAVLTPGIYNSAYYEHSFLAQQMGVELVEGRDLVAVNNRIMMRTTKGFEPVDVL
                     YRRIDDDFLDPQAFRSDSLLGVAGLMEIYKKGRVALANAPGTGIADDKSIYAYVPKII
                     EYYLGEKILIPNVPTHICAEPEELSYVLANLNRLVVKAVNESGGYGMLVGPHSSSGEC
                     AEFARRIRENPRNYIAQPTLGLSRVPVIADGRVVGRHVDLRPYVLYGSDIYVLPGGLT
                     RVALKPGSLVVNSSQGGGSKDTWVLQA"
     gene            3733..4713
                     /locus_tag="VE170_16265"
     CDS             3733..4713
                     /locus_tag="VE170_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HYJ17047.1"
                     /translation="MLSRVANSIYWLSRYMERADNVARFIDVNLQLMLDLPSGSQEQW
                     HPLVSISGGDVAFSAKYPMATRDNVIKFLTFDGDNPSSIVSCLRAARENARTIRESIS
                     PEMWEQVNGFYLTVNAAAANERLRMSSSSFFSDVKKASQLFHGITDATMLHGEVWHFY
                     RLGRLLERADKTSRLLDVKYFMLLPSVADVGTPLDDIQWAAVLRSASALEMYRKRHRH
                     IAPEQVINFLVLDHEFPRSIHYCLTTANDSLHAISGTPIGMYRNTAEQRLGQLRAELA
                     YTDTPQIVARGLHQFLDGFQTKLNLVDQCIFDTFFALRPVGSSAPDRVGQ"
     gene            4760..5617
                     /locus_tag="VE170_16270"
     CDS             4760..5617
                     /locus_tag="VE170_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HYJ17048.1"
                     /translation="MFFEISHKTTYTYSRPIFLELHHLRIRPRCDGVQTVTYFESTVE
                     PCPAGSTDGIDIDGNIVENVWFHGLTEKLIVASRAKIITQYRNPFNYILAQRADRLPV
                     SCQDPLQPYLAPYTVRRNESHLIEQFARHVANRTEWKTLRFLTMLTREISESFQQTDR
                     GVGGPRSAEETYLSRAGSSRDLAVLFIDACRYVGIAARFVNGYYHDGGAKERLSLHSW
                     GEVYLPGVGWRGYDPSQGLAVTDCHVAIAAAGDPLLSAPVSGTFRGNAITSELTAEIE
                     ITVAGADAA"
     gene            complement(5647..7359)
                     /locus_tag="VE170_16275"
     CDS             complement(5647..7359)
                     /locus_tag="VE170_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ17049.1"
                     /translation="MHGGQVMLKAIERSGIDFILSSPGSEWPPVWEALAEAAARGAQK
                     PKYINCRHEAVAVAMAAGYTKVTQRPQLVMLHATAGPLNAAMTLRAALHERTPMVIIS
                     GEIAAYGENTKVADPGGQWLHDLTDLGGSPDLLRGCVKWADRISSGELLGPTIERAVR
                     IAMEPPMGPVLIGIPFECMMEEVADIDHGRANEVARAQAVDDASIDKALSLIAAAKNP
                     LVLTEHVGGDPRAVERLVELCELFGIPVMETYRPAFMNFPRTHPLYLHHDPKAVDAAD
                     LILMVDAVTPWYPASKAPKNAKVIAIADEFPNSRLPYWGYNVDLALVAAPASTLGQLV
                     QKAKASEQVAANRTHYLARSARAGEQHDSYFGKLKEEAQKHANDTPIDPRWAVHALSE
                     ALPKNAVISEETTVYRSLIQETMPRDQPQSYFARVTGGLGVGLSYALGVKLALPDRPV
                     FAFIGDGAFHYNAVPSCLGVAEEYSLPIHCVIFNNGRYLSMETSLIKYFPDGAAKTTG
                     VHFGAEIGPRPDYQHYAKAHGGEGFRVTNPQEIKNAIEQALKCEKEKKLTVTDLVLSD
                     LNPR"
     gene            complement(7736..8755)
                     /locus_tag="VE170_16280"
     CDS             complement(7736..8755)
                     /locus_tag="VE170_16280"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17050.1"
                     /translation="MTGKQTWILAVVLLAISFPGATVRAAVPTPMLAAYGGHNETMIP
                     MWVGADKGLFRKYGVDLRALQTRSGPIMMATLVSGGTPLVWAAPSSALSTTASGLKLG
                     CFAVGNNQVPREVIVRKGIDTIEDLRGKSFGVQSIGGGFWISTMVVLDALGIDPDKYK
                     LNMRVIGDTATVTQALITGNVDAMVVPYSYGDIAKRAGARALADAGKLNVTYQATVMC
                     AQKDSGAVSNDTMIAITKGIVDALAYILEPGNKREVSEVLKKNLRLSKDEDVDASYRV
                     SRLQMPNLDVAPNLEAWRTVKRLVAKVNPKVQDVDLEQVIVTGPAQFLETSGFMAEMR
                     KRLPR"
     gene            complement(8793..>9023)
                     /locus_tag="VE170_16285"
     CDS             complement(8793..>9023)
                     /locus_tag="VE170_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17051.1"
                     /translation="NFSRQGAKTPSSELWSFRPEGEIFLDPSSLIEMTGISPTALRLC
                     AAFAGEFPAIDFRYAALTTARHFALGKYFRLW"
BASE COUNT         1817 a         2594 c         2666 g         1946 t
ORIGIN      
        1 gcaatgaaaa agccgcatta ccgtccgtgg cgatcgatcc gtcaaggttc aacgccgagg
       61 taggcattgc gcacgtattg caggaccatg cgctgggtgt gaaagtatga accgttgagc
      121 gagatcgccg agcgcctgat tgccgcatag gcgtcgggct gtcggtagta gagcggcaag
      181 atcgcggatt ctaacttgtc atagagtgag tcggcctcgc tggcgagatc cgccacgcca
      241 tcgtcgccga tcgcccaacc ggtcacgcct tcgacgtggc cttcgaccca ccaaccatcc
      301 aatacgctga ggctcggcac gccgttgagg gcggccttca tgccgctggt gccggaggct
      361 tccagcggtc tttccggcgt gttcaaccag agatcgaccc cggcgcacag tgatcgagcc
      421 aggtccatgt cataattttc caaataaacg acgcgaagcg agtctttcag ttttttcgaa
      481 aattcgaaaa tgcgccggat catggctttg cccgtttcat cgtgagggtg agccttgccg
      541 ctgaaaataa tctgaaaggg tccagagcgc tcggtgattg cctccagccg ttccaggtcg
      601 gtaaatagca aatcggcgcg tttatagccg gcagcgcgtc gagcgaagca aatggtcaag
      661 acattttccg agagatgcgc tttggtccgc agggcgatct gcgccagcat ttcgcgtttg
      721 gcgtgggcat gtgcgcgttg gacgtcggcg agggggattt tgatcgcgta acgcaaatag
      781 aaactgtcgc ggcgccactc gggaatgaag cggtcgtaca aattgcgaaa cggtgcggcg
      841 gtccaggtca gggcatggac gccgttggta atcgaatcga tggcgtgatc gggaaacagg
      901 tcgcgggaaa tttccccatg gcgcatggcc acgccgttga tataacgcga cagatggagc
      961 gccaggtagg tcatattgag catgccgcct tgaatgcact ggagcttctc tagagccgcc
     1021 aaacgcgtgg ctcccaaaac ctcgctgacc agcgcgacgg ggaactggtc atggccggcc
     1081 ggcaccggcg tgtgagtggt aaagacgctg cgttgccgaa ccgcttcgca atcggtttcc
     1141 gccaccgcag ccaaatcgcc gcgcgccgcc tcgcgctcca aaagcgccaa agtcagaaac
     1201 gccgcatggc cctcgttcat atggtgggtg ctgacattct tgtagccaag cgcctgaagc
     1261 atcgccaccc cgcccatgcc caagaccact tcctggcaaa gccgatggcg tccgtcaccg
     1321 tcgtacagag actcggtcaa gctttgttcc cagggggtat tttcctttaa gtcggtgtcg
     1381 aggaaataga ccggcacggt atggccgaag acgccgcgca cgatgtagcg ccaggcgcga
     1441 accatgacgg cccgcccggc gatcggcacg aaggcgcgcg gcaccatcgg ttccaaaact
     1501 tcttcgtagt tccagccggc aggactctcg gtttgattgc cgccggcgtc gagagattgg
     1561 cggaaatagc ctttgcgatg cagcagtgtg acgccgatca tcggcagacc gaggtcggcg
     1621 gccgcgcgta gcgtatcgcc ggcaagaacg cccaagccgc cactgtaggt cggcatcgac
     1681 gagtcaaggc ccacttccat ggaaaaatag gcgacatagt ttggtaggat ttcttttgta
     1741 aacattgctt cccccggtgc gttgcgcggc cgtcgacgga tcgcggatta agccattggg
     1801 gtaataaagc cagctctttt tgacggtgct gatttaacta gtgcgagagc tagcagaaaa
     1861 acctggtgca caaagcatac cagcacctcg cggcgccatc agtttcacca tatgggcgat
     1921 ccacacggca atcccaacga gcaggacatt tatggctcaa ttaatatgca gcgatggctc
     1981 aaaaaacggc aaatgttgat ggacgggggt gacggttgat ttttgttctt gagtatccac
     2041 catgggaaat tggccacaac aatcgttgat caaagctcag gcagcgcatt gcccaaaaat
     2101 tggtcgatcg ccgcaaagcc aggcaattaa cgggcgcaaa gttcgcgccc tgatatggca
     2161 tattcgttgc ggtagacaag gggtgctgcg gtcgtgttat tttgtcctgg cgagggcggc
     2221 tttgcgattt cactggcacc ggcaccatga aatttgaact ttacgaaacc actgatttct
     2281 atgacgagat gttcgtctcc ggcagcgccc ctcgcgctgg cgcgcgactg ttgaaggagc
     2341 gcatcgaatc gctgccggac ggtgagctgt tcgcccgcca agcggccgcc gaaagcctgc
     2401 tcctgcaaaa cggcattacc ttcaacgttt atagcgacag cgcggccacc gaaaaaatat
     2461 ttcccttcga tgtcataccg cggatcgtcg aagccgccga gtgggattgg atcgaaaagg
     2521 gattgaagca gcgggtttac gctctcaatc tattcatcga cgatatttat catcagcaaa
     2581 aaatcgtcaa agacaaagtc gtgccggaat atctcatcca atcggcgcgg tctttcagga
     2641 aagaatgcat cggactaaac ccgccgcaga aggtctggtg ccatatcacc ggcaccgatc
     2701 tggtgcgcga ccgcgacggc cagatttacg tcctggaaga taatttgcgc tgtccctccg
     2761 gcgtatctta tgttttggaa aaccgccgga tcatgaagcg gacctttccc caggtcttcg
     2821 aggcttcgcg cgtgcggccg gtggatgaat atcccagccg tctgctatcg accctccagt
     2881 cgcttttccc agcggccatc gactcgccca aagtcgctgt gttgactccg gggatttaca
     2941 attcggccta ctacgaacat tcttttttgg cgcaacaaat gggcgtcgaa ttggtcgagg
     3001 gccgcgatct ggtggcggtc aacaaccgga tcatgatgcg caccaccaaa ggtttcgagc
     3061 ccgtcgacgt gctgtaccgg cgcatcgacg atgattttct cgatccgcaa gctttccggt
     3121 cggattcgtt actgggagtg gcgggcctca tggagattta taagaaaggg cgggtggcgc
     3181 tggcgaacgc gccggggacg ggcattgccg acgacaagtc gatctatgcc tacgtgccga
     3241 agatcatcga atattatctc ggcgaaaaaa tcctaatccc aaacgtgcca acccatattt
     3301 gcgccgaacc cgaggaactt tcttacgtgc ttgccaacct gaaccgatta gtcgtcaaag
     3361 cggtcaacga atcgggcggt tacggcatgc tcgtggggcc gcactcgagc tccggtgaat
     3421 gcgctgagtt tgcccggcgg attcgagaga atccgcgcaa ctacattgcc cagccgacct
     3481 tgggtctatc gcgggtgccg gtcatcgccg atggccgcgt cgtcggccgc catgtcgatt
     3541 tgcgtcccta tgtgctctac ggcagcgaca tctacgtgct ccccggcggg ttgacccgcg
     3601 tcgcactcaa accgggctcc ctggtggtca actcgtcgca gggcggaggg agcaaagaca
     3661 cgtgggtatt acaggcttaa acgccgcgcg cctcagcagg tgcctggagc ggcggaatcg
     3721 tcaatcagac caatgctaag ccgagtcgcc aattcgatct actggttgag ccgatacatg
     3781 gagcgcgccg acaacgtcgc ccgtttcatc gatgtcaacc tgcaattgat gctcgatctg
     3841 ccgagcggct cgcaggaaca gtggcatccc ctggtgagca tctccggcgg cgacgtggcg
     3901 ttctcggcga aatatccaat ggcaacgcgg gataacgtca tcaagtttct tacctttgac
     3961 ggggataacc caagctcgat cgtgtcatgc ctgcgcgctg cccgggagaa cgcccggaca
     4021 attcgtgaat cgatctcgcc cgagatgtgg gaacaggtca acggttttta tttgacggtc
     4081 aacgcggcgg cggcaaacga gcggctgcgg atgtcgtcgt cgagcttttt tagcgacgta
     4141 aaaaaggcta gccagctgtt tcatggcatc accgacgcga ccatgttgca cggtgaagtg
     4201 tggcacttct atcgcctggg acgactgctc gaacgcgccg ataagacctc ccggctgctc
     4261 gatgtcaagt atttcatgct gttgccgtcg gtcgccgacg tgggtacgcc gttggacgat
     4321 atccaatggg cggcggtatt gcgatcggca agcgctttag aaatgtaccg caagcgccat
     4381 cgccacatcg cgccggagca agttatcaac tttctggtct tggaccatga gtttccgcgc
     4441 tcgatacatt actgcctgac gaccgccaac gattcgctgc acgccatctc cggcacaccg
     4501 atcggcatgt accgcaatac cgccgagcag cgcttgggcc agttgcgtgc cgagttggcg
     4561 tataccgaca cgccgcagat cgtcgctcgc ggcctgcatc aatttctcga cggctttcag
     4621 accaagttaa atctggtgga tcaatgcatc ttcgatacct ttttcgcgct gcgcccggtg
     4681 gggtcgagcg cgccggatag ggtcggccaa tgaccggcaa ggcaatatcg cggcaatgtt
     4741 ggtggccgga aggataacca tgttcttcga aatcagtcat aagactacct acacttacag
     4801 ccgcccgatt tttctcgaat tgcatcattt gcggatccgt ccgcgctgcg atggcgtaca
     4861 aactgtgact tacttcgagt ccacggtgga gccatgcccg gcgggatcga ccgacgggat
     4921 cgatatcgac ggcaacatcg ttgagaatgt ttggtttcac gggttgacgg aaaaattgat
     4981 cgtcgccagc cgcgccaaaa ttattaccca ataccgcaat ccgtttaact atattctcgc
     5041 ccaacgggcc gaccgtttgc cggtcagctg tcaggatccg ctccagcctt atctcgcgcc
     5101 ttataccgtg cgccgaaacg aaagccattt aatcgaacaa ttcgcccgtc acgttgccaa
     5161 tcgcaccgaa tggaaaactt tgcgcttcct gaccatgcta accagggaaa tttccgaaag
     5221 ctttcaacaa accgaccgag gcgttggcgg gccgcgcagt gccgaagaaa cctatttgag
     5281 ccgagccgga tcgagccgcg atctagcggt gttattcatc gatgcttgcc gttacgtcgg
     5341 catcgcggcg cgtttcgtca atggttacta ccatgacggc ggcgccaaag agcgactctc
     5401 gctgcactcg tggggcgaag tctatttacc cggcgtcggt tggcgcggct acgatccaag
     5461 tcagggtctg gcggtgaccg attgccacgt ggccatcgct gccgctgggg atccgctact
     5521 gtcggcgccc gtaagcggga cgtttcgcgg caacgcgatt acgtcggaac tcactgccga
     5581 aatcgagatc acggttgccg gcgccgacgc ggcgtaagcg cgggctgttt ccggctcttg
     5641 tacaatttag cgcggattca aatcgctcag caccaggtcg gtcaccgtca atttcttttc
     5701 tttttcgcat ttcaacgctt gctcgatggc gtttttgatt tcctgcgggt tggtgacccg
     5761 gaagccttcg ccgccgtgag ctttggcgta gtgttggtag tccggccgcg ggccgatctc
     5821 ggcgccgaag tgaactccgg tggtttttgc cgcgccgtcg gggaaatatt tgatcaagct
     5881 ggtttccatc gacagataac ggccgttgtt gaagattacg cagtggattg gcaggctata
     5941 ttcctccgcc actcccaggc atgagggcac ggcgttgtaa tgaaacgcgc catcgccgat
     6001 gaacgcaaag accggccggt cgggcagcgc taacttcacg ccgagagcgt agctcaagcc
     6061 gacgccaagt ccgccggtga cgcgcgcgaa ataggactgc ggttggtcgc gcggcatggt
     6121 ttcctggatc aaactgcggt aaaccgtggt ctcctcggag atcaccgcat tcttcggcag
     6181 ggcctcgctg agcgcatgca ccgcccaacg cgggtcgatt ggcgtgtcgt tggcgtgctt
     6241 ctgcgcttcc tccttcagct tgccgaaata gctgtcatgc tgttcgcccg cgcgcgctga
     6301 gcgcgccagg tagtgggtgc gattggcggc aacctgttcc gaagctttgg ctttttgcac
     6361 tagctgtcct agggtcgatg ccggcgcagc gaccaaggcg agatcgacgt tgtagcccca
     6421 gtagggcagg cgcgagttgg gaaattcgtc ggcgatggca atcactttgg cgttcttcgg
     6481 cgctttgctc gccggatacc acggcgtcac ggcgtcgacc ataagaatca aatccgccgc
     6541 atcgaccgcc ttgggatcgt gatgcaagta gagcgggtgg gtgcgcggga aattcataaa
     6601 cgccggtcgg taggtttcca ttaccgggat gccgaatagc tcgcaaagct cgaccaggcg
     6661 ctccacggcg cgggggtcgc cgccgacatg ttcggtcaat accaggggat ttttcgccgc
     6721 ggcgatgaga ctcaatgcct tatcgatgct ggcatcgtcg acggcctggg cgcgggctac
     6781 ttcattggcc ctgccgtggt cgatgtccgc gacctcttcc atcatgcatt cgaacggaat
     6841 gccgatcagc accggcccca tcggcggttc catggcgatg cgcacggcgc gttcgatggt
     6901 tgggcctagt aattctccgg acgaaatgcg gtcggcccat ttgacgcagc cgcgcagaag
     6961 gtcgggcgag ccgcccaggt cggtgagatc atgcagccac tggccgccgg gatcggcgac
     7021 ttttgtgttc tcgccgtagg cggcgatctc accgctgatg atcaccatcg gcgtgcgctc
     7081 gtgcagcgcg gcgcgcaagg tcatggcggc attgagcgga ccggcggtgg cgtgcagcat
     7141 gaccagctgc ggccgctggg tcaccttggt gtagccggcc gccatggcga cggcgaccgc
     7201 ttcgtggcgg caattgatgt acttgggctt ctgcgcgccg cgcgccgccg cttcggccag
     7261 cgcctcccag accggcggcc actccgaacc cggcgaggat agaataaaat cgatgccgct
     7321 ccgttcgatc gccttcaaca ttacctgccc gccgtgcatg gtggtctcct tttgcgcgtt
     7381 atgagcgcgg ttcagttttc aaagttcaaa agttcaaggt tgattctcgg ccttgcacca
     7441 cgaagacgcg aagtatacga agttcaaaaa agaaatctcc tgtcttctct taccttcgtg
     7501 atcctcgtgc ctacttcgaa aatacttgta aggggatggt cgccaaaatg aagagggtat
     7561 ctgggttccg tcatacccgc gaaagcgggt atccaggcta attgggcgca gatcaaacct
     7621 ggattcccgc gtgcgcggga atgacccgtc gagtagactg cctatggggc gaatcccatg
     7681 cattttcata ttctgagcgt agatcatgat tcacttcgtg gtgaacagag tctcgtcatc
     7741 gcggcagtcg ctttctcatc tccgccatga agccgctggt ttccaaaaat tgcgccggcc
     7801 cggtaacgat cacttgttcg aggtcgacgt cttgcacttt gggatttacc ttggcgacca
     7861 gccgtttcac cgtgcgccag gcttccaggt tcggcgcgac atcgaggttg ggcatttgca
     7921 agcgcgagac tcgataggac gcgtcgacat cttcgtcttt gctcaagcgc aggttttttt
     7981 tgagcacctc actgacttcg cgcttgttgc ccggttcgag aatgtaggcc agcgcatcga
     8041 cgatgccctt ggttatggcg atcatggtgt cgttcgatac cgcgccggaa tctttctggg
     8101 cgcacatgac ggtggcttga taggtgacgt tcaacttgcc ggcatcggcc aatgccctgg
     8161 cgccggcgcg tttggcgata tcgccgtagc tatagggcac gaccatggcg tcgacgttgc
     8221 cggtaatcag cgcttgggtg acggtggcgg tgtcgccgat gacgcgcatg ttgagtttgt
     8281 atttgtccgg gtcgatgccc aaagcgtcga gcacgaccat ggtcgagatc caaaagccgc
     8341 cgccgatgct ctgcacgcca aagctcttgc cgcgcaggtc ttcgatcgtg tcgatgcctt
     8401 ttctgacgat gacttcccgc ggcacctggt tgttgccgac ggcgaagcag ccgagcttga
     8461 gtccgctcgc cgtggtgctc agcgcgctgc tcggcgccgc ccagacgagc ggcgtgccgc
     8521 ccgacacgag ggtcgccatc atgatcggtc cgctgcgggt ctggagcgcg cgcaggtcga
     8581 cgccgtactt gcggaacagt cctttgtccg cgccgaccca catcggaatc atggtttcgt
     8641 tgtggccgcc gtaggccgcc agcatgggcg tcggcaccgc ggcgcgaacc gtagcgccgg
     8701 gaaaagatat tgccaagaga accacagcca atatccatgt ttgtttgccg gtcataggtt
     8761 tggctcctat ttgttccagt cagtccccgc tactaccaca gccgaaagta tttgcccagc
     8821 gcgaaatgcc tcgcagttgt aagcgcggcg tatctgaaat cgatagccgg aaattctccc
     8881 gcaaaggcgg cgcaaagccg caaagctgtt gggctaatgc ccgtcatctc gatcaaactc
     8941 gacggatcta ggaagatttc tccctccggt cgaaatgacc acagttccga acttggcgtc
     9001 tttgcgcctt ggcgggagaa att
//
LOCUS       DASQKS010000629         5614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2885504, whole genome shotgun sequence.
ACCESSION   DASQKS010000629 DASQKS010000000
VERSION     DASQKS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885504"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VE170_16290"
     CDS             <1..447
                     /locus_tag="VE170_16290"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYJ17052.1"
                     /translation="SAFQDQGSVHHCHGCGAANEKGLQLKSYWDGDSAVAQWRAAAHH
                     CGGTRDNLNGGIIASLIDCHSLNLAIAHAYRAEGRAIGSVPRIGYVTAELKVSNRKPT
                     PLQETIELRAWMRRLEGRKAWVDCTLSAAGVICATGDVLGVRVEWN"
     gene            559..1503
                     /locus_tag="VE170_16295"
     CDS             559..1503
                     /locus_tag="VE170_16295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ17053.1"
                     /translation="MDYTTLGKTGLKVSVAGLGCGGPSRLGMRGDVGAEDHAVRLIRQ
                     ALDLGVNFLDTAQNYGTEGVVGKAIAGVARDRVVISTKKTLPTPDDADPAAAIVQGLE
                     KSLKLLGTDYIDIYHLHGVEPKDYEFAQNQLIEPMRRLQQQGKIRFVGVTEGFVVDTT
                     HTMLADSLKKELWDVVMVGFSLLNPSARKHVFPLTQKSGVGVLDMFAVRRALSQPQRL
                     KEMCAELVEKGAIEKTALDLDDPLGFLLKETDAATLPEAAYRFCRHERGVDVVLTGTG
                     NADHLKENIAAILKAPLPQASLEKLARLFGGLDYLTGN"
     gene            complement(1615..2724)
                     /locus_tag="VE170_16300"
     CDS             complement(1615..2724)
                     /locus_tag="VE170_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ17054.1"
                     /translation="MKLPGKATPDGTERYQQRFTQTLPPGHFRRSQNLCLSSIGLGTY
                     LGNYDDATDQEYHQAIVKAVGAGCNVVDSAINYRLQRSERSIGTALKALSSRGYNRDE
                     IVVASKAGFIPYDGTPPKDPRAYIKTAFIDSGLATAADIISGCHCMTAKYLLNQLDCS
                     LRNLDVETIDIYYLHNPETQLGKVSAEEFDQRVVKAFEALEGAVEAGKIQMYGLATWN
                     CFRNDADAKDYLSLAHVVELAEQAGGKNHRLKVIQLPLNLGMSEALSNASQPAHGHSH
                     TVLEVAQDLGMTVMCSASVLQGQLSRNLPPIVAETFTGLDTDAQRALQFVRSTPGVTT
                     ALVGMKQQKHVEENLQTARVPPAPWEQYSKLFQTN"
     gene            complement(2721..3203)
                     /locus_tag="VE170_16305"
     CDS             complement(2721..3203)
                     /locus_tag="VE170_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ17055.1"
                     /translation="MNLKQRTYLWYLAVLRIYVGYYLFFQGIGKYRRNFPKGDWIGRQ
                     IGDIASLDLYPWYKTFLQRYVVPHSELFGYLVMIGEIAVGVGLLLGLFTRLSALVGLF
                     MVANYYLGPGSARGGAMLAQQQTFIIMLAIFVLAGPGRTLGLDGLSFSSAGAKGKNAK
                     "
     gene            complement(3273..4040)
                     /gene="gloB"
                     /locus_tag="VE170_16310"
     CDS             complement(3273..4040)
                     /gene="gloB"
                     /locus_tag="VE170_16310"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HYJ17056.1"
                     /translation="MKIHQIPLLRDNYGYLVVCEKTNQAAIVDPSEAEPVLRRIEQEK
                     VEPVAILNTHHHRDHTGGNPGLLATHKLKVYGHTSDRERVFGMTDGVDEGATVQIGEL
                     KGSVFFIPGHTTGHVAYLFDNNLFCGDTLFTAGCGRLFEGTPQQMHASLKKLMALPDN
                     TKVYCGHEYTESNLRFAMTVEAKNPKLAARFEHVQGLRARGTPTVPSTLDEEKLTNPF
                     LRWDSKEIQASVKAAAPGSQDDPVSVFAAVRKLKDAF"
     gene            4228..>5614
                     /locus_tag="VE170_16315"
     CDS             4228..>5614
                     /locus_tag="VE170_16315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17057.1"
                     /translation="MENRAAQIHAELKHPVIDGDGHWMEPIPVFLEYLSEVGGPKSVD
                     QMRALWRARDAWYRATWPERQHQRLRRAIWWGVTSNTLDKATALLPALLNERLPELGI
                     DFALIYPSFGLSINGMAQGDLHRAAVRAYNTMTADMFAPYAARFAPVAIIPSHTPDEA
                     LDELEYAVRQRGFKAVMLRGNQERTIPAAAEGINERGRAEWYCDTIALDSPYDYEPFW
                     QRCVDLGVAVTQHSGSGRWQDRASISNFTYNHVGHFAESNHAFARGVFLGGLVRRYPS
                     LNFGFMEGGVSWACQMCADMIEHWEKRRRAGLQYPDATSVAEMHQLIGRYGDQRLKAN
                     ADAIMNSLDAFRPECSLEELARPEHVSDDFEAAGINSKEDVRAVFSGNFYFGCEADDR
                     ATMWAFDPRMGVRLRPVFSSDFTHFDVPDFREVIPEAFEMVEKGFVTAQDFREFTFTN
                     AARLHTRNNPEF"
BASE COUNT         1140 a         1561 c         1736 g         1177 t
ORIGIN      
        1 agcgcttttc aggaccaggg ctcggtgcat cactgccatg gctgcggcgc cgccaatgaa
       61 aagggattgc agctgaaaag ttactgggat ggcgatagtg cggtcgccca gtggcgcgcc
      121 gcagcgcacc actgcggtgg cacacgggat aatttaaacg gcgggatcat cgcctcgctg
      181 atcgactgcc atagcttgaa tctggccatc gcccacgctt accgcgccga agggcgtgcc
      241 atcggtagcg tcccgcgtat cggttatgtg acggcagagc tgaaggtttc gaaccgcaag
      301 ccgacgccgc tgcaagaaac catcgaactt cgtgcgtgga tgcgccggct cgaaggccgc
      361 aaggcttggg tcgattgcac gttgagcgcg gcgggagtca tttgcgccac cggcgacgtg
      421 ctcggcgtgc gagtggaatg gaattgaatg caagttcaag gttcaaggtt caaaagttca
      481 acgattcgga gtttaatcgg agtctggtaa ctttgaacct tgaactttta aacctttgaa
      541 cggatcgaga atttttcgat ggactacacg acactcggta aaaccggatt gaaagtcagc
      601 gttgccgggc ttggctgcgg cgggccgagc cggctcggca tgcgcggcga tgtcggtgcg
      661 gaagatcacg cggtgcgcct gatcaggcag gcgctcgatc tcggcgtcaa ctttctcgac
      721 acggcgcaga attacggcac cgagggggtc gtcggtaaag cgatcgccgg cgtagcgcgc
      781 gaccgcgtgg tcatctcgac aaaaaagact ttgcccacgc cggacgatgc cgatccggca
      841 gcggcgatcg tccaaggctt ggagaagagt ttgaaacttc tcggcaccga ctatatcgat
      901 atctatcact tgcacggcgt cgagccgaag gattatgagt ttgcccaaaa ccagctgatc
      961 gagccgatgc gccggcttca gcagcagggc aagatccgct tcgtcggcgt caccgaaggg
     1021 ttcgtcgtcg ataccacaca tacgatgttg gccgacagtc tcaagaaaga gctctgggac
     1081 gtggtgatgg tcggcttcag tttgctcaat ccgtcggcgc gaaagcatgt cttcccgctg
     1141 acccagaaaa gcggcgtcgg cgtgctcgat atgttcgcgg tgcggcgcgc cctcagccag
     1201 ccccagcggc taaaagaaat gtgtgctgaa ctggtcgaga aaggcgccat cgaaaaaacc
     1261 gcgctggatc tcgacgatcc gctcggtttc ctgctcaagg aaaccgacgc tgcgacatta
     1321 ccggaagcgg cctaccgctt ttgccgccac gagcgtggcg tcgacgtcgt cctcaccggc
     1381 accggcaacg ccgatcactt gaaagagaac atcgccgcga tcttgaaagc gccgctaccg
     1441 caagcgtcgc tcgagaaact tgcgcgactg tttggcggct tggattatct gacgggcaat
     1501 tagccagcga atttcgcata gactcaccgg cgcttgccgc tcaccgtagc ccgctcccgc
     1561 ccttgcgggc gcggtaatct taaagaaaag tcgcaaatag aatcaccgag gacgttagtt
     1621 cgtctggaac aatttcgaat attgttccca gggtgccggg gggacgcgcg cggtctgcaa
     1681 attttcctcg acatgttttt gctgtttcat gccaaccagc gcggtggtga cgcctggcgt
     1741 cgagcggacg aattgcaggg cgcgctgggc gtcggtatcg agcccggtaa aagtctcagc
     1801 gacgatcggc ggcaggttgc gactgagctg gccttgcaga accgaggcgc tgcacatcac
     1861 cgtcatgccg agatcctggg cgacctcgag cacggtatgg ctatggccat gggccggctg
     1921 actggcgttg gacagcgctt cgctcatgcc cagattgagc ggtaactgga tgaccttgag
     1981 tcgatggttt ttgccgccgg cttgttcggc caactcaacg acatgggcca gggataagta
     2041 atctttggca tcggcgtcgt tgcgaaaaca gttccaggtc gccaagccat acatctgaat
     2101 cttgccggct tcgacggcgc cttccaatgc ttcgaaagct ttgaccacgc gctgatcgaa
     2161 ctcctcggcg ctcactttgc ccaactgtgt ttccgggtta tgcagatagt agatatcgat
     2221 ggtttcgacg tcgagatttc gcaagctgca atcgagctgg ttgagcaggt attttgccgt
     2281 catgcagtgg cagccgctga tgatatcggc ggcagtggcg aggccggagt cgatgaatgc
     2341 cgtcttgatg taggcgcgtg gatccttagg gggtgtgccg tcgtaaggga taaagccggc
     2401 tttgctggcg acgacgattt cgtcgcgatt gtagccgcgg ctggacaacg ctttaagcgc
     2461 ggtgccaatg gagcgttcgc tgcgctgcaa ccggtagttg atggcgctgt cgacgacgtt
     2521 gcaaccggcg ccgacggctt tgacgatcgc ttggtgatat tcctgatcgg tggcgtcgtc
     2581 gtagttgccc agataggtgc ccaggccaat ggaggagaga cagagatttt gcgaccggcg
     2641 aaaatggcct ggcggcagtg tctgcgtgaa gcgctgttgg taacgttcag tgccgtcggg
     2701 cgtcgctttg ccggggagtt tcattttgca ttctttcctt tagcgccagc gctgctaaat
     2761 gatagaccat cgaggccaag tgtgcggccg ggaccggcca ggacgaaaat cgccaacatg
     2821 atgatgaagg tctgctgttg cgccagcatc gcgccgccgc gggccgagcc gggaccgaga
     2881 taatagtttg ccaccataaa caggccgacc agcgcgctca agcgcgtgaa taagcccaat
     2941 agcagaccaa cgcctacggc aatctcaccg atcatgacga gatagccgaa aagctcgcta
     3001 tgcggcacga cataacgctg caagaaagtc ttgtaccacg gatagagatc gagactggcg
     3061 atgtcgccga tctgccggcc gatccaatcg cccttgggaa agttgcgccg atattttccg
     3121 atcccctgaa aaaacagata gtaaccgaca taaatgcgca gcacggcgag ataccagagg
     3181 taagttcgtt gttttagatt catcgcgccc tcgctttggc taacgagccg cgccgatgtg
     3241 ataatgccgc ggcattgggg gaatcgccgg gattagaatg cgtctttcag tttccgcacg
     3301 gcggcgaaaa ccgacaccgg gtcgtcttgg ctgccggggg cagcggcttt tacgctggct
     3361 tggatttcct tgctgtccca gcgcaaaaac ggattggtca gcttttcctc gtccaaggtc
     3421 gacggcaccg tcggggtgcc gcgcgcgcgc agtccctgaa cgtgctcgaa gcgcgccgcc
     3481 aacttgggat ttttcgcttc gacggtcatg gcaaagcgca gattgctctc ggtatattca
     3541 tggccgcaat agactttggt attgtccggc agggccatga gttttttcag cgaggcgtgc
     3601 atttgctgcg gtgtgccttc gaagagccgg ccgcaaccgg cggtaaacag cgtgtcgccg
     3661 caaaaaagat tgttgtcgaa cagataggcg acatggccgg tggtgtggcc gggaatgaag
     3721 aatacgctcc ccttgagctc gccgatttgg accgttgcac cttcgtcaac gccgtcggtc
     3781 atgccgaata cccgctcgcg gtcgctcgtg tggccgtaaa ctttcagctt atgcgtcgcc
     3841 aggaggccag ggttgccgcc ggtatggtcg cgatggtgat gcgtgttcag gatcgccacc
     3901 ggctcgactt tttcttgttc gatgcggcgc aataccggct ccgcttccga cggatcgacg
     3961 atggcggctt ggttggtttt ctcacacacg accaggtagc cgtagttatc gcgcaggagc
     4021 gggatttgat gaatcttcat ggggttttcc taatctgggt aggggtaaac tgctgctgaa
     4081 ctctagtcgg aaccgcgcgg tttttcaaat cccaattttt tcctgtcgtc cctcggcggt
     4141 gatgcaacgc gatcgattcg attgcgattg acgacgcgcc ggcttagcga ttatagagga
     4201 ggtaaattta ccagaggagt ggcgactatg gaaaatcgag ccgcacagat acacgccgaa
     4261 ctgaagcatc cggtgatcga cggcgacggc cattggatgg agccgattcc ggtcttcctc
     4321 gaatacttga gcgaagtggg cggacctaaa tctgtcgacc agatgagggc gctctggcgc
     4381 gcgcgggacg catggtaccg ggccacctgg ccggagcgcc agcaccagcg cttgcgccgc
     4441 gccatctggt ggggtgtgac cagcaacacg ctcgacaagg caaccgcgct gttgccggcc
     4501 ttgctgaatg agcggttgcc tgaattgggt atcgactttg cgctgatcta tccgagcttc
     4561 gggctcagca taaacggcat ggcccagggc gacctgcacc gggcggcggt tcgcgcctac
     4621 aacacgatga cggctgacat gttcgcgccg tatgccgcaa ggtttgcgcc ggtagccatc
     4681 attccctcgc atacgccgga tgaagccctc gacgagctcg aatacgcggt caggcagcgt
     4741 ggttttaaag cggtcatgct gcggggaaac caggaacgca cgatcccagc cgcggcggaa
     4801 ggaatcaatg agcggggcag ggctgaatgg tactgtgata ccatcgcctt ggacagcccc
     4861 tatgactatg agccgttttg gcagcgctgc gtcgacctcg gcgtcgccgt cacccaacac
     4921 tcaggcagcg gccgttggca agatcgcgcg tcgatcagta acttcaccta caaccacgtc
     4981 ggccacttcg ctgaatcgaa ccacgccttc gcgcgcggcg tttttcttgg cggcctcgtg
     5041 cgacgttacc cgagtctcaa tttcggcttc atggaaggcg gcgtgagctg ggcctgccag
     5101 atgtgcgccg atatgatcga acactgggaa aagcgccgcc gtgccgggct gcagtacccg
     5161 gacgcaacca gcgtcgccga gatgcatcaa ttgatcggcc gctatggcga ccagagactg
     5221 aaagccaacg ccgacgcgat catgaacagc cttgacgcat tccggccgga gtgcagtctt
     5281 gaagagctcg ctcggcctga gcatgtttcg gacgactttg aagcggcagg aattaactca
     5341 aaagaggacg tccgggcggt cttctccggc aatttttatt tcggctgcga ggctgacgac
     5401 cgggcaacca tgtgggcctt cgatccgcgc atgggcgttc ggctgcggcc ggtgttcagc
     5461 tccgacttca cccatttcga cgtgccggat ttcagggagg tgattcccga agcgtttgag
     5521 atggtcgaga agggcttcgt gacggcgcag gatttccgtg agtttacatt caccaatgcc
     5581 gcgcgtctgc acacccgcaa taacccggaa tttt
//
LOCUS       DASQKS010000630         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5403026, whole genome shotgun sequence.
ACCESSION   DASQKS010000630 DASQKS010000000
VERSION     DASQKS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5403026"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..523)
                     /locus_tag="VE170_16320"
     CDS             complement(107..523)
                     /locus_tag="VE170_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HYJ17058.1"
                     /translation="MNAYAKPLPRTDVDTQPFWDGCKLHDLRAQRCSDCRRFRWPPQA
                     FCPECYSWKFEWTRLAQTGKVATFVVVHYAAVAAFQPDLPYVVAHIVIDGTAGRVEIV
                     SNVIGCPWQDVKVDMAVKAVFEDVTPDVTLAKFEPA"
     gene            complement(526..1707)
                     /locus_tag="VE170_16325"
     CDS             complement(526..1707)
                     /locus_tag="VE170_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17059.1"
                     /translation="MPEQAIKDKTAIAGIGWTAFTRGSGVRTLTLAAEASLNAVADAG
                     LEVQHIDGIVSYFHKRPESQEVRELTQMLGIKNCNFQLYSDGGGGWNCAAVLSAAMLV
                     HSGVCKNVLVFKAQNRYSEGRAARAERVHDVSGPDQFTMPFGQHHAAAQFGPHATAHM
                     ARYGTTSLDFAHLAVTERSHAMLNQKAMMRKPMTVADHQASRWIIYPFRLFDCCQETD
                     GAVALVVTSAERARDLKHAPVYIMAGVGGTSQTAGWWETNGINTAPQLYEGAGIAPRD
                     VSIAEIYDPFTFMCMTHLEDLGVVPKGEIGPWIRAGNNRLDGSLPVNTHGGLLSEGHI
                     AGLNHFIEAVQQLRPEGVVDDLCEGTHTYDRATCRQVRDPQIALLCGECVDSAMLLRK
                     A"
     gene            1930..3015
                     /locus_tag="VE170_16330"
     CDS             1930..3015
                     /locus_tag="VE170_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17060.1"
                     /translation="MMFPAQFKLLFLAALVAIAGPSVTASPAATVEEIALMKSPNREK
                     TLIDGAKKEGKVTFYTGLIVDQVVRPVKDAFEKEYPFVQVEFFRANADRLAQRVLAEH
                     QAKRYEVDIVSGSAAATMLQRAGIMQRFYSPAIAEYPRELKDANGFWGSTNVYFMTLG
                     YNTRLVKAAELPKSYEDLLNPRWKGQMMWSTSRGSGAPQFVGNIFTTMGQEAGKAYLQ
                     KLKQQNIAKSTASARQILDLVIAGEYPLSIQIFNHHAFISKSAGAPVEWHPLEPVTAT
                     NNSIGIAKTAPHPHASMLFLDFVLSRRGQRVFQAANYLPAHPEIPALQADLKPGVRFK
                     KANYLSPELLYDKSNEWADYFEKEFLR"
     gene            3057..3773
                     /locus_tag="VE170_16335"
     CDS             3057..3773
                     /locus_tag="VE170_16335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ17061.1"
                     /translation="MKITPSAPPKIVFVPETRIGFWFLGTQTWTRHVLRVALDDLERL
                     MPQRRPCYPIVLDAGCGQGKSFRLLIERFSPQRLIGVDAEEKCLQHAHVEALKEKIPI
                     ELRHGDIAALDVGDDSVDLVFCHQTFHHLSRQQLALEQIFRVLKPGGLLLFAESTRAY
                     ICSWMIRLLFRHPMHVQRSAAEYQAMIRDQGFTFGSENVSLPYLWWSRSDLGTFEWFG
                     FGVPAQREETLVNLVAVKPD"
     gene            3928..4356
                     /locus_tag="VE170_16340"
     CDS             3928..4356
                     /locus_tag="VE170_16340"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HYJ17062.1"
                     /translation="MEYHALPMLLSRIALVVACAASSGCMSLRQENCRSEAQFAIHNS
                     LYFGTAKPDGVVTAEQWAEFLEAVITPRFPQGLTVFRASGQWNMEDGTMAREATYVVQ
                     IIHPNEERFEKNIRELVAIYKEQFQQESVLLVTARSCVSF"
     gene            4790..>5114
                     /locus_tag="VE170_16345"
     CDS             4790..>5114
                     /locus_tag="VE170_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ17063.1"
                     /translation="MNADIEDVKREVATANRVLANLGLATGFTASLGHASMRVATSPK
                     HFFVKGREYQYDALELMEPDDMVMCDTEGFKIGGRSELTQCSEVKIHACIYKTHPNVQ
                     AVVHVH"
BASE COUNT         1083 a         1450 c         1475 g         1106 t
ORIGIN      
        1 tctgtatcct tcttccgaga gtctaaaacc aaaataaaaa actattcgcc attgctcgcg
       61 aattcacgcg ggataaacgg gtgcgccctt tatttcgctc gttacattac gccggctcaa
      121 acttcgccag cgtcacgtcc ggcgtgacgt cttcgaacac cgccttgacc gccatgtcga
      181 ctttgacatc ttgccacggg cagccgatga cattgctgac gatctcgact cggccagcgg
      241 tgccgtcgat gacgatatgc gcgacgacgt agggtaggtc gggttgaaag gccgcgactg
      301 cggcgtagtg aaccaccacg aaggttgcaa ccttgccggt ctgtgccagt cgggtccatt
      361 caaacttcca cgaataacac tcgggacaaa acgcttgagg cggccagcgg aaccgacggc
      421 agtcgctgca gcgttgggcg cgcaggtcgt ggagcttgca gccatcccaa aacggctgcg
      481 tgtccacatc ggtacgcggc agcggtttgg cgtaagcgtt catactcaag cctttctcaa
      541 cagcatggcg ctgtcgacgc attcgccgca cagtagtgcg atctgcggat cgcgcacctg
      601 gcggcaggtg gcgcggtcgt aggtgtgcgt cccttcgcag agatcgtcga cgacaccttc
      661 ggggcgtaac tgctgcaccg cctcgatgaa atggttgagg ccggcgatat ggccttccga
      721 caacaggccg ccgtgggtgt tgaccggcag cgagccatcg aggcgattat ttccggcgcg
      781 aatccatgga ccgatctcgc cttttggcac cacgcccaag tcttcgagat gagtcatgca
      841 catgaatgtg aacgggtcgt aaatttcggc gatgctgaca tcgcgtggcg caatgccggc
      901 gccttcgtaa agctgcggcg cggtattgat gccgttggtt tcccaccaac cggcggtctg
      961 gctcgtgccg ccgacgccgg ccatgatgta gaccggcgcg tgtttcaaat cgcgcgcccg
     1021 ctcggcggaa gttaccacca gcgccaccgc gccgtcggtt tcctggcagc agtcgaacaa
     1081 gcgaaatgga taaatgatcc agcgcgacgc ctggtggtct gccacagtca tcggtttgcg
     1141 catcatcgct ttctggttga gcatcgcgtg gctgcgctcg gtgacggcga gatgagcgaa
     1201 atcgagcgac gtggtgccgt agcgcgccat gtgggcggtg gcgtgggggc cgaattgggc
     1261 cgcggcatgg tgctggccga agggcatcgt aaactgatcc ggaccgctga catcatggac
     1321 gcgctcggcc cgcgccgcgc gtccttcgct gtaacgattc tgggctttga acaccaagac
     1381 gtttttacag acacccgagt gcacgagcat ggcggcggat aacactgcgg cgcagttcca
     1441 gccgccgccc ccgtcgctgt agagctgaaa gttgcaattt ttgatgccca acatctgcgt
     1501 cagctcgcgc acctcttgcg attccggccg tttatgaaag tacgaaacta tgccgtcaat
     1561 atgctgcacc tcgagtccgg catcggcgac ggcgttgaga ctcgcctcgg cggccaaggt
     1621 cagcgtgcgc acccccgatc cgcgtgtaaa cgccgtccag ccgatcccgg cgatggcggt
     1681 cttatccttg atggcctgct ccggcattga attttctccg tgaaattcgc cgtgaatatt
     1741 tgctccgtta atttggccgg accatagcat gaattgccta accggcaaca gaaaatcagc
     1801 tcgctgtttc tggtactgcc gcgccaacaa atccttgaca gcaaggtgac ggcgtggcta
     1861 gcttgcgcca tgaagtcgtt agtagctatg cgtcagatga tcgcctaacc agcagtcgca
     1921 ggaggatcga tgatgtttcc cgctcagttc aagcttttat tcttggctgc gctcgtcgcc
     1981 attgcagggc cttccgttac cgcatcacca gcggcgacgg tggaagaaat tgccttgatg
     2041 aaatcgccca atcgcgaaaa gaccctcatc gacggcgcca agaaagaggg caaggtgacg
     2101 ttctataccg gactgatcgt cgatcaggtg gtacggccgg taaaggatgc cttcgagaaa
     2161 gaatatccgt ttgttcaggt cgagttcttt cgcgccaacg ccgaccgcct ggcgcagcgg
     2221 gtattggccg agcaccaagc caaacgttat gaggtcgata tcgtcagcgg cagcgccgcc
     2281 gccaccatgt tgcagcgcgc cgggataatg cagcgtttct attcgccggc catcgccgag
     2341 tacccgcgcg agttgaaaga tgccaacggt ttctggggca gcaccaacgt ttacttcatg
     2401 accctgggct acaacacgcg cctggtgaaa gcggccgagt tgccgaagag ctatgaagac
     2461 ctgctcaatc cacgctggaa aggccagatg atgtggtcca ccagccgcgg ctcgggcgcg
     2521 ccgcagttcg tcggcaacat tttcacgact atgggccagg aagcgggcaa ggcctatctg
     2581 caaaagttga agcagcagaa catcgccaag agcaccgcca gcgcgcgcca gatactcgac
     2641 ctggttatcg ccggcgagta cccgttatcg attcagattt tcaaccatca tgctttcatc
     2701 agcaaatccg ccggcgcgcc ggtggagtgg catccgctcg aacccgtgac cgcgaccaat
     2761 aattctatcg gcatcgccaa aaccgcgccc catccccatg cttccatgct gtttctcgac
     2821 ttcgtgcttt cgcgcagagg ccaacgggta tttcaagccg ccaattactt gccggcccat
     2881 cccgaaattc ccgccctgca agccgacctg aaacccggcg tgcgcttcaa gaaagcgaat
     2941 tatctcagcc ctgagttgct ctacgacaag agcaatgaat gggcggatta ttttgagaag
     3001 gagtttttgc gctgaccccc gggtttgacg actcgatgaa ctctcggttc gtaggcatga
     3061 agataactcc cagtgctcct cctaagatcg tcttcgtacc ggagaccagg attggctttt
     3121 ggtttctcgg cacccagact tggacccgcc atgtactgcg agtcgcgctt gacgatttgg
     3181 aacgactgat gccgcaacgc cggccatgct atccgatcgt gctcgacgca ggatgtggcc
     3241 aaggtaagtc cttccggcta ctgatcgaac gtttctcgcc gcagcgcttg atcggtgtcg
     3301 acgctgaaga gaaatgtctc caacatgccc acgtagaggc gttaaaagaa aaaattccca
     3361 tcgaactccg gcacggcgat atcgcggcgc tggatgtcgg cgatgatagc gtggacttgg
     3421 tattctgcca ccagaccttt caccatctat cgcgccagca attggcatta gagcaaatat
     3481 ttcgcgtgct caaacccggc gggttattgt tgttcgccga atcgacgcgc gcctatattt
     3541 gctcctggat gattcggctg ttgtttcgcc acccgatgca tgtgcagcgg agcgcggcgg
     3601 agtaccaggc gatgattcga gatcagggtt ttacttttgg ctccgagaat gtgtccctgc
     3661 cttacttgtg gtggagccgg tctgatctcg gcacgttcga gtggtttggc ttcggcgtgc
     3721 cggcgcagcg ggaggagacg ctggtcaacc tcgtggcggt gaaacccgac tagcggcgcc
     3781 tcgctgccag ttggtcggaa gcgggctcgg atattctcaa ccgcaggagc atcacgtgga
     3841 tgggtcaatt cggacaagcg ttgattgcgg tgagactgcg cgaattttca tactaaatcg
     3901 tgttgcccgt agctaaaatg ttgattgatg gaatatcacg ccttacctat gctgttgtca
     3961 cggatcgcgc tcgttgttgc ttgtgcggcc tcgtccggtt gtatgtcgct gcgccaggaa
     4021 aattgtcgga gcgaggcgca gtttgccatt cataattcgc tgtacttcgg cactgccaag
     4081 ccagacggcg tggttacggc agagcagtgg gcagagtttc ttgaagccgt catcactcct
     4141 cggtttccgc aaggccttac cgtgtttaga gcttccggac aatggaatat ggaggatggc
     4201 acgatggccc gagaggcaac ctacgtggtg caaattattc accccaacga ggaacgcttc
     4261 gaaaagaata ttcgcgaact cgtcgcgatc tacaaggaac agtttcaaca agagtcggtc
     4321 cttctagtga cggcccgttc gtgcgtgtct ttctagtcgc tgcattgcca cggacggaac
     4381 aaagcctcga tcagcgatgg ctaagccaaa gactgggtcg atagtacggc gatgacgact
     4441 gcttagaaag cagtcttccg ttcaactatt cgattctcta cagccacaaa agtcttcgat
     4501 tgtcgtagac aaacttctgc acggcggttg gcgagatagg cgaaatatca tgggcgtcgg
     4561 cgcgcttatt gatcaaaccc aggaattcgg gttcgtcacg gagccgcgct gcgaattatt
     4621 ttaacctgcc tgtgacttca gactaaagcg catgccttcg aagccggtct tgacagtatg
     4681 gcccagcttc gatcagtgta gcggtcgcgg cagatgatca agcagcacaa atacaatagg
     4741 tacaaccagc gggcgcgata gagcgcgctc gccgtagaag gagctagcaa tgaatgcaga
     4801 tattgaagac gtaaagcgcg aggttgcgac ggcgaaccgt gtgctcgcca atcttgggtt
     4861 ggcgaccgga tttaccgctt cgctgggaca cgcgagcatg cgcgttgcca cttcgccaaa
     4921 gcattttttc gtcaaaggac gtgagtacca gtacgacgcc ctcgaactga tggagcccga
     4981 cgacatggtc atgtgcgaca ccgaaggttt caagatcggc ggccggagcg aactgacgca
     5041 gtgctcggaa gtcaaaatcc acgcctgcat ttacaagacg catccgaacg tgcaagcggt
     5101 cgtgcacgtg catc
//
LOCUS       DASQKS010000631        10070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_322807, whole genome shotgun sequence.
ACCESSION   DASQKS010000631 DASQKS010000000
VERSION     DASQKS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322807"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VE170_16350"
     CDS             <1..664
                     /locus_tag="VE170_16350"
                     /inference="COORDINATES: protein motif:HMM:NF020694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HYJ17064.1"
                     /translation="KAALKSGRKDLLYCDANAISPMTADAIAKVLAPAGVHFVDGCII
                     GSAAKMGKGTIVYLAGPQAEQLRGLEVFNIPLKILGPSTNQASAFKVVYAGLTKGLQG
                     LFCELLMGARKFGLLDEIRAQYEESFPGLLDKVSSSIVGLQIHAGRRAEEMDELKRTF
                     NSHGLDSFMAPAAQKVLRAIAALNLSQASDSGVRQGELADTLELFYQKGLLQQKSPAD
                     IS"
     gene            735..2504
                     /locus_tag="VE170_16355"
     CDS             735..2504
                     /locus_tag="VE170_16355"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ17065.1"
                     /translation="MSRVEKKQPEFGSDVVVDLMKAHDIEYTAFNPGATFRGIHDSIV
                     NYGGNYKPEVIFCHHEEISVAIAHGYAKAKGKPMIAICHNMVGLQHASMAIFNAYIDR
                     VPMLVLGGTGPMNTKRRRPRIDWIHTALVQGNQVRDYTKWDDQPYSLADVPDSFIRGY
                     RIATTDPTAPVYINYDADIQEDAVKAMVDMPDVSRHSTPAPAQANPEALRRTAEMLVN
                     AKNLLIVADMLGRNPKAVPMLIELAEVLGAPVVDKGARFNFPAVHPLDVTDGAREILQ
                     KADFILALDVGDLFGALTTVSKQTRESEYVTTPGVRIASISMNDMLVHSWANDFQALQ
                     AIDIPMTADTSVAVPELTRLCRELIGKDSAKKATIEARQKELSEKHKSRRAKWLSDAQ
                     AKGSQKDISTAWLALEIGEAIKREDWVCVNGTSNGWTRRLWDLSKPNQSLGGSGGAGL
                     GYGPGAAIGAALALKGSGKLAVAIQSDGDMLMTSSAIWTAAKHRIPLLMIMHNNQSYY
                     NSEEHGIEVAKFRKRPVENAPIGTHVDDPAIDFAMMAKSFGVNGEGPVRNPAELRPAI
                     ERGVKYVKEKQLPYLVDVIAEPR"
     gene            2626..3459
                     /locus_tag="VE170_16360"
     CDS             2626..3459
                     /locus_tag="VE170_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ17066.1"
                     /translation="MVTDLYREFSKAVAGPEETIDLGRAALTIALGDYPDLDIENYLR
                     RIDALAVAVNQRCAADSDMFHSIAALNFVLFNDQGFRGNRDDYYDPKNSFLNDVIDSR
                     RGLPITLSVLYMEVGQRIGLTLDGVGFPGHFLVKTIVEGDEIVIDPFNAGEIKSHEDL
                     QTMLDRLYGGQVRFHRDFLAPLPKKQILRRMLGNLKAIYGRANELVKMLAVLDRLIIV
                     DPGAVEEVRDRGAVYLRLECYGQAKDDFETYLRLAPDAKDANAIRDQLIDLAKQTVRI
                     H"
     gene            complement(3570..5780)
                     /locus_tag="VE170_16365"
     CDS             complement(3570..5780)
                     /locus_tag="VE170_16365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ17067.1"
                     /translation="MLQQVEKFHIVLIKPSKYDDDGYLIRFWKGVLPSNTLNVLHGLT
                     EDLKERRVFGKLDIEVTTFDETAEKVPVGKIIRWSKAPGTKLLVCMVGVQTNQFPRAV
                     DMSRLFRAENITVIMGGFHTSGTINMLSDQEPDIQELFREGICVVSGEVEGKWEGILA
                     DYLHGQLKPLYSFAQDLQNLVDIGEAPLPRISAKTMRHFAYPSFATADTSRGCPFSCS
                     FCTIINVQGRKMRERSPASIAAMMRRNLENGLSFYFFTDDNFARKKLWRETFEEIIKL
                     REQGLDITFMMQVDLARKPKEFVNLSAKAGCTQVFIGMESVNPKNLAAEDKKQNKVDE
                     YRAIIKEWHDAGVLVHTGYIIGLPWDSKEQVAEDMRFLKDEVEPDQASFFMLTPLPGS
                     HDHREMKKRGDWMDPDFNKRDSFHATIAHPLMSAQEWTEAYEAAWKTFYSKENMIKSL
                     SKWTDNPKNYWNLMAIFFWYKNAALIEKQHPMVAGFFRLKDRKVRRPGHSIDSLPVHL
                     WKRAKENTTLLITWAKFVKEMEEVWLQTRKKSENEERWLEQIQKTQSDIWRALRIGEI
                     QNIYNNARDSLPERVKTLLAPLDDLSSRILYSRNDLNRFLKQWNSLCQNIQELRLMEP
                     ETARKWLDEMHQIQSGIRFGDRINEWQETYQRLRDSLPSKAQLSLIKYDAINDRVVYS
                     RQELQRIWSESVNNLKSYKPWAIRPLQLARAAVKDLFLTRSFAADFKEFSKLGA"
     gene            5936..6625
                     /locus_tag="VE170_16370"
     CDS             5936..6625
                     /locus_tag="VE170_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ17068.1"
                     /translation="MIQFQHVAKRYAPNLCALDDINLEIHKGELVLLSGASGAGKSTL
                     LKLVYREEEPSAGKVFVEGRNLQSFNGRAVAKLRRRVGLVFQEFKLLANLSALENIAF
                     AAEIAGTARKAAQARAEQLLGELGLASRRHAKPDGLSGGEQQRVAVARALVNRPALIL
                     ADEPTGNLDATAAAETLRLFKDLRQQDSTIVIASHDARFFEEIASRMVHLEKGRIVAD
                     TAGSGAVEWRL"
     gene            6745..7536
                     /locus_tag="VE170_16375"
     CDS             6745..7536
                     /locus_tag="VE170_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYJ17069.1"
                     /translation="MLQLNVEKLLRGLGDQMQVTAYLAPGLDADELQALLARLQGFPG
                     IAHVRFTSQEQAWRDFQASLGAQSALLEGLPRDVLPASVEISVRPNFRDSVAMEHLAV
                     LLRQQKELTQIDYPQQWVERLSLVGLALQWAKWFVAGVLFLTTFFLVRNMVKLAVMAR
                     REEIEVMQLVGASEALIQAPLAIEGLVQGLLGAGGGLAALWGFYRVLRDDVAGLSELV
                     PRLGGIEFLDGPRIALILTIGVVLGASASLFAVRGMVRSWHASAR"
     gene            7515..8681
                     /locus_tag="VE170_16380"
     CDS             7515..8681
                     /locus_tag="VE170_16380"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYJ17070.1"
                     /translation="MACLRALIGACLLVLAGSRAVPGAVASKDLDGMKRRIESEKKGL
                     SQLQIKEGGALKALGKIEEDLDRKSKYLKVTDDQYRAILGLIEKTETEARQVRGSMSQ
                     REALFRQRVTALYRWQRSSAPLRFAQGASVLGETLKRHRYFAATLAFDRGLIGEMHSE
                     TQRQESLRRQLSEQKDRLAEQKRLLGLARAAVAQEMDKKKILLISLRQEKDYRLKVLR
                     DMAAAAQRLQKMLDEIARRTIVRPKDLPAPSFPGRGLDNLLGQLDWPVKGSVSAPFGK
                     FKHPAFAAEVIRKGIDIDASAGDGVRAVEKGRVVFADRFTGYGRMVIIDHGERYYTIY
                     GHLSEIIRKNGEELARGEILGRVGDSDSLSGAKLYFELRKDGRSVDPLPWFKKR"
     gene            8745..>10070
                     /locus_tag="VE170_16385"
     CDS             8745..>10070
                     /locus_tag="VE170_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYJ17071.1"
                     /translation="MGLAKRHGTPLLLIVLGIGIGFFIAGQWVPNVSAVGRQDYESLE
                     AFSNILSIVKKNYVEDVETKNLVNGAINGMLSSLDPHSAYLTPDLYKDLQSDTQGRFG
                     GLGIEITVKGGMLTVVSPIEDTPAHKAGIKPGDQIFKIEDEFTKDMSLVDAVKKMRGL
                     KGTKINLTIRRENATDLIDVSLVRDIIRVQSVKSRSLEPGYGYVRLAQFQERSDRDVQ
                     RALEKLAAEASGLKGLVLDLRNNPGGLLNQAVRVSDLFLDSGMIVYTDGRIESQKQKF
                     FAQKEGTWMDFPLVVLVNGGSASASEIVAGALQDHKRAVVLGTKSFGKGSVQTILPLD
                     DNSALRLTTARYFTPKGRSIQATGIVPDILIDNIPLQDGKPDDRKRPSLREENLPGHL
                     NNPLQNSAKEQEQIDREREKQIPTSGPTGDETIDNDQQLKRALDLLKSWD"
BASE COUNT         2204 a         2675 c         2967 g         2224 t
ORIGIN      
        1 caaggcggcg ctcaagagcg gcagaaaaga tttactttac tgcgatgcca acgcgatatc
       61 gccgatgact gccgacgcga tcgccaaagt gctcgctcct gccggagtgc attttgtcga
      121 tggttgcatc attggttccg cggccaagat gggcaagggc acgattgtct atcttgccgg
      181 gccgcaggcc gagcagctgc gcggattgga agttttcaac attcccttaa agattcttgg
      241 tccgagtacc aatcaggcat cggcgttcaa agtcgtctat gccggactca ccaaggggct
      301 gcagggatta ttttgcgagt tgctcatggg ggcgcggaag tttggcttgc tggacgagat
      361 tcgcgctcag tacgaagaga gttttcctgg tttgctcgac aaggtttcat ccagcatcgt
      421 tggcttgcag attcacgccg gccggcgcgc cgaggagatg gatgaattga agcgcacctt
      481 taactcccat ggtctcgatt cgttcatggc gccggcggcg cagaaagttt tgcgcgcgat
      541 tgccgcgttg aatctgagcc aagcttccga cagcggcgtg cgccagggcg aactggcgga
      601 tactctggaa ttgttttacc aaaaaggtct tttacaacag aaatcgccag ctgacatttc
      661 ttaaatcagc cctagctagg cggcgggttt cctggtattg agagcgaaac agtaattaga
      721 gggaggagct agcaatgtcc agagtcgaaa agaaacaacc ggaattcggt tccgatgtcg
      781 tggtcgatct aatgaaagcc catgacatcg agtacaccgc gttcaatccc ggtgcaacct
      841 tccgtggaat ccacgactcc atcgtcaact atggcggcaa ctacaaaccc gaagtgatct
      901 tctgccatca tgaagaaatc tcggtggcca tcgcccacgg ctacgctaag gcgaagggca
      961 aaccgatgat cgctatctgc cacaacatgg tcggtttgca gcatgccagc atggcgattt
     1021 tcaacgccta catcgaccgg gtgccgatgc tggtgctcgg cggcacgggg ccgatgaaca
     1081 caaaacggcg ccggccgcgc atcgactgga tccacacggc gttggtgcag ggcaaccagg
     1141 tgcgcgacta cacgaagtgg gacgatcagc cttactcgct agccgatgtg cccgattcat
     1201 ttatccgggg ctaccgcatc gccaccaccg atccgaccgc gccggtctat attaactacg
     1261 atgccgatat tcaagaagat gcggtcaaag ccatggtcga catgccggat gtgagccgtc
     1321 attccacgcc ggcgccggcg caggccaatc ccgaggcgct gcggcgcacc gccgagatgc
     1381 tcgtcaatgc caagaatctc ttgatcgtcg ccgacatgct cgggcgcaat cccaaagccg
     1441 taccgatgct gatcgagctg gccgaagtgc tgggcgcgcc ggtggtcgat aaaggcgcgc
     1501 gtttcaattt tccagcggtg catccattag atgtgaccga cggcgctcgg gaaattctcc
     1561 agaaggctga ttttatcttg gccctcgacg tcggcgacct attcggcgcc ttgaccacgg
     1621 tgagcaagca gacccgtgaa agcgagtatg taaccacgcc cggcgtgcgc atcgccagca
     1681 tttcgatgaa cgacatgttg gtgcattcct gggccaacga ctttcaggcg ctgcaggcga
     1741 tcgatatccc aatgaccgcc gatacttcag tggcggtgcc ggaactgacc cggctgtgcc
     1801 gcgaactcat cggcaaggac agcgccaaga aagcgaccat cgaagcgcgc caaaaagaac
     1861 tatcggagaa gcacaagagc cgccgcgcta aatggctgag cgacgcccag gcgaaaggct
     1921 cgcagaaaga catctcgaca gcgtggttgg cgctcgagat cggcgaagcg atcaagcgcg
     1981 aagactgggt ttgcgtaaac ggcacttcga atggctggac gcgccggctt tgggacttaa
     2041 gcaaaccgaa tcaatccctc ggcggcagcg gcggcgcagg cttgggttat gggcccggcg
     2101 cggcgatcgg cgcagccctg gcgctcaagg gcagcggcaa gctcgccgtg gcgattcagt
     2161 ccgacggcga tatgttgatg acgtcgagcg cgatctggac ggcggctaaa caccgcattc
     2221 cgctgctcat gatcatgcac aacaaccagt cctactataa ttcggaagag cacggcatcg
     2281 aagtcgctaa atttcgtaaa cggccggtgg agaatgcgcc gatcggcact catgttgacg
     2341 atcccgcgat cgatttcgcg atgatggcga aatcctttgg cgtcaacggc gaaggcccgg
     2401 tgcgcaaccc ggccgagctc cggccggcca tcgaacgcgg tgtcaagtac gtcaaggaga
     2461 agcagttgcc gtacttggtc gatgtcatcg ccgaaccgcg ctagagaaat tgcggttaac
     2521 agggaaattc aggctgaatt caccgcaggg ttattgtgta ggggcggaaa atttttcgcc
     2581 cctacagccc tgtaacggac actgacccaa ggcagcctcg ccaaattggt taccgatctt
     2641 taccgagaat ttagcaaggc ggttgcgggg ccggaagaaa ccatcgacct aggccgcgcc
     2701 gcattgacca tcgccctcgg cgactatccc gatctcgaca ttgaaaacta ccttcggcgc
     2761 atcgatgcat tggccgtcgc ggtcaatcaa agatgcgctg ccgattcgga tatgtttcat
     2821 tcgatcgccg cgctcaactt cgtgttgttc aacgatcagg gctttcgcgg caatcgtgat
     2881 gattactatg accctaagaa tagttttttg aatgatgtga tcgacagcag gcggggcctg
     2941 ccaatcacgc tatcggttct ctatatggaa gtgggtcaac gcatcggtct cacccttgac
     3001 ggcgtcggct tccccggcca ttttttggta aaaacgatag tcgagggcga tgagatcgtc
     3061 atcgatccat tcaacgccgg agagatcaag tcgcacgaag acttgcagac catgctcgat
     3121 cgactctatg gcggtcaagt acggttccat cgggactttc tcgcgccact gccaaagaag
     3181 cagattctca ggcgcatgct cggtaacctg aaggcgatct atggccgcgc caacgagctg
     3241 gtcaaaatgc tcgcggtgct cgaccgtctg atcattgtcg atcctggtgc ggtggaagaa
     3301 gtgcgcgacc gcggcgccgt ctatcttcgg cttgagtgct acggccaagc gaaggatgac
     3361 ttcgagactt atctgcggct ggcgccggat gccaaagatg ccaatgcaat tcgggatcag
     3421 ctcatcgact tggccaagca gaccgtacgg atccactgac ggtcaagtcc gctgagagat
     3481 ttgccccgcc ggtttatgaa ttcttttagc taagcgtaga cgatgtagag tcgccgcggc
     3541 cgggtattgt tgatgcacct aaacaggatt taggctccta gtttagaaaa ctccttgaag
     3601 tcggcggcga aggagcgggt cagaaataga tctttgaccg ccgcacgggc taattgcaaa
     3661 ggccggatcg cccaaggttt gtaggattta agattgttta cgctctcact ccagatgcgc
     3721 tggagttcct ggcgtgaata gacgacccgg tcattaatgg cgtcatattt gatcaggcta
     3781 agttgcgctt ttgagggcag cgagtcgcgc agccgctggt aggtctcctg ccattcgttg
     3841 atgcggtcgc caaagcgaat accgctctgg atctggtgca tctcatcgag ccatttgcgc
     3901 gcggtttccg gttccattag tcgaagttcc tggatgttct ggcacaggct attccattgc
     3961 ttcaagaagc gattcagatc gttccggctg taaagaattc gtgacgaaag atcgtcgagc
     4021 ggggccagca gcgtttttac ccgttccggc aaggaatccc tggcgttatt gtaaatgttt
     4081 tgaatctcac cgatgcgtaa tgcgcgccaa atgtccgact gagttttttg gatttgttcc
     4141 agccagcgct cttcgttctc gctctttttg cgggtttgca gccagacttc ttccatctcc
     4201 ttgacgaatt tcgcccaagt gatcagtagc gtcgtgtttt ctttggcgcg tttccacaga
     4261 tgcacgggga gcgaatctat cgaatggccg ggacggcgaa ctttacgatc tttcaagcgg
     4321 aagaaaccgg cgaccatggg atgttgcttc tcgatcaagg cggcattctt gtaccagaag
     4381 aaaatcgcca tcaggttcca gtagtttttc gggttgtcgg tccacttgga caaggacttg
     4441 atcatgttct ctttgctata aaatgtcttc caggcggctt cgtaggcctc ggtccactcc
     4501 tgcgccgaca tcagggggtg ggcgatggtg gcatggaacg aatcgcgctt gttgaagtca
     4561 gggtccatcc aatcaccgcg cttcttcatc tcgcggtggt cgtgcgagcc gggcagcggc
     4621 gtgagcatga aaaaagatgc ctgatccggc tccacttcgt ctttgaggaa acgcatgtct
     4681 tcggcgacct gctctttgga gtcccaaggc aagccgatga tgtagccggt atgcaccaac
     4741 acgccggcgt catgccattc cttgatgatg gcgcggtatt catcgacttt attttgcttt
     4801 ttgtcttcgg cagccaagtt tttcgggttg acgctctcca tgccgatgaa cacctgggta
     4861 cagccggcct tggcggagag attgacgaac tctttaggct tgcgggccag atccacctgc
     4921 atcatgaaag taatgtcgag cccttgttca cgcagcttga tgatctcttc aaaggtctcg
     4981 cgccagagtt ttttacgcgc gaagttgtcg tcggtgaaaa aatagaagga taggccgttc
     5041 tcgaggttgc gccgcatcat cgctgcgatg ctcgccggac tgcgctcgcg catcttgcgc
     5101 ccctggacgt tgatgatggt gcagaagcta caagagaacg gacagccgcg ggaagtgtcg
     5161 gcggtggcga aactcgggta ggcgaaatgg cgcatggttt tcgcgctgat tcgcggcaat
     5221 ggcgcctcgc cgatgtcgac cagattttgc agatcctggg cgaagctata cagcggcttt
     5281 agttgcccgt gcagataatc cgcgagaatg ccttcccatt tgccttcgac ttcgccggag
     5341 actacgcaga tgccttcacg gaaaagctct tggatatccg gctcctggtc gctcagcatg
     5401 ttgatggtgc cgctggtatg gaagccgccc atgatcaccg tgatgttttc ggcgcgaaac
     5461 agccgcgaca tgtcgacagc gcggggaaat tgattggttt gcacgccgac catgcacacc
     5521 agtagtttag tgccgggcgc tttgctccag cgaatgattt tacccaccgg aactttctcg
     5581 gcggtctcat cgaacgtggt cacttcgata tcgagcttgc cgaacacccg gcgctctttg
     5641 agatcttcgg tcaggccatg caggacgttg agcgtattac tcggtagcac gcctttccaa
     5701 aagcgaatga ggtagccatc atcgtcgtac ttggagggct tgatcaaaac gatatgaaat
     5761 ttttcgactt gctgcagcat cggttctgtc ccctcccgaa tgcttgtaac tgtttggagc
     5821 atcttttata gatgattgag gagcttaaag tcttacagaa tttaacaacc tgggttggtg
     5881 gtgtcaagcc aaaaactctt gtcaatcacg actttcccat ccaattttca ttggaatgat
     5941 ccaattccag catgttgcca agcgctatgc gccgaaccta tgcgccctcg acgacatcaa
     6001 tcttgagata cataagggcg agttagtgct tcttagcggc gctagcggcg ccggcaaatc
     6061 gaccctattg aaactcgtct atcgtgagga ggaaccgagt gcgggtaagg ttttcgtcga
     6121 gggtcggaac ctccaatctt tcaatggccg ggccgtcgcc aagctacggc gcagagtcgg
     6181 actcgtcttt caggaattta aactgctagc gaacttgagt gcgttggaaa atatcgcttt
     6241 cgccgcggaa attgccggca cggcgagaaa agccgcacag gcgagagccg aacagttatt
     6301 gggagagctt gggttggcgt ctcgccgtca tgccaaaccc gacgggctgt ccggcggaga
     6361 gcagcaacgg gtcgcggtgg cccgggcgct ggtcaatcgg ccggcactta ttctggccga
     6421 cgaacccact ggcaatcttg acgctacggc ggccgctgaa acccttcgac tcttcaaaga
     6481 tctccggcag caggatagta ccatcgtgat tgccagccac gatgctcgct tctttgaaga
     6541 gatcgccagt cgaatggttc acctcgagaa gggtaggata gtcgccgata cggccggcag
     6601 cggcgcagta gagtggcgcc tctgatggct acgctcggct ttatcgcacg gcgcgcgggt
     6661 catggactgc gacagtttct gggcgccgtt attctaatca gttttacaat tgcattgaca
     6721 ctcggcgtat ttggcgggtt tctgatgctc caactcaatg tcgaaaaact attgcgggga
     6781 ttgggcgacc aaatgcaagt gaccgcttat ttggctccgg gcctcgacgc cgacgagttg
     6841 caggctttgc tagcgcgtct gcagggcttc ccgggaatcg cccatgtgcg ttttaccagt
     6901 caggagcagg cctggcgcga ttttcaggcc agtttagggg cccagtcggc gttactggaa
     6961 gggttgccgc gcgatgtctt gccggcctcg gtcgaaattt ccgtgcggcc gaatttccgc
     7021 gatagcgtcg cgatggaaca tctggcggta ctgctgaggc agcaaaagga attgacccaa
     7081 atcgattatc cgcagcaatg ggtcgagcgg ctgagcttag ttgggttggc attgcagtgg
     7141 gccaaatggt tcgttgccgg agtgctcttt ctcaccactt ttttccttgt caggaacatg
     7201 gtgaagctgg cggttatggc gcggcgggaa gagatcgaag tgatgcagct ggtcggcgcg
     7261 agcgaagcgc tgattcaagc cccgttggcg atagaagggc tggttcaagg gctgctcggt
     7321 gcgggcggcg gcctggcggc actttgggga ttttatcgtg tgctgcgtga cgacgtcgcc
     7381 ggcctgtccg agctcgttcc taggctcggc ggcatcgagt ttctcgatgg gccaaggatc
     7441 gccttgattc tgaccattgg cgttgtgctc ggtgcttcgg caagtctatt cgcggtgcgg
     7501 ggaatggtgc gctcatggca tgcctccgcg cgttaatcgg cgcgtgcctg ttagtgttgg
     7561 ctggttctag agcggtgccg ggcgcggttg ccagcaagga tctcgatggc atgaagagaa
     7621 ggatcgagag tgaaaaaaaa ggcttgtcgc agctgcaaat caaagaaggc ggcgcgctca
     7681 aagccctcgg taaaattgaa gaggatctcg acaggaagtc caagtatctc aaggtaaccg
     7741 atgaccaata ccgcgcgatc ctcggcctga tcgaaaagac cgaaaccgag gcgcgccagg
     7801 tgcgcggatc gatgagtcaa cgcgaggcgt tgtttcggca gcgcgtgacc gcgctctatc
     7861 gctggcaacg cagcagcgct cctttaaggt ttgcccaggg cgccagcgtt cttggcgaaa
     7921 ccttgaaacg ccatcgttac ttcgcggcga cattggcttt cgaccgggga ttgattggcg
     7981 aaatgcacag cgaaacgcag cgccaagaaa gtttgcggcg ccaactgagc gagcagaagg
     8041 atcggttggc ggagcaaaaa aggctcttgg ggctggcccg cgcagcggtg gcgcaggaaa
     8101 tggataaaaa gaaaatactc ttgatcagtt tgcgtcagga aaaagattac cggctcaaag
     8161 tcttgcgcga catggctgcg gcggcgcagc ggctgcagaa aatgctcgac gagattgccc
     8221 gccggacgat tgtccgtcct aaagacttac ctgcgccgtc cttccccggg cgcggccttg
     8281 ataatctgct tgggcagttg gactggccgg tgaagggttc agtgagcgcg ccatttggca
     8341 aattcaaaca tccggcgttc gctgccgagg tgatccgcaa gggcatcgat atcgacgcgt
     8401 cggcaggcga cggcgtgcgc gcagtggaaa aaggtcgtgt ggtattcgcc gaccggttta
     8461 ccggctacgg ccgcatggtg atcatcgatc atggcgagcg gtactatact atttacggtc
     8521 atctttccga aattattaga aaaaatggcg aagagctggc ccgtggcgag atcctcggac
     8581 gggtcggcga tagcgactcg ctgagtggcg ccaagctata ttttgagctg cgcaaggacg
     8641 gtcgctcagt cgatccgttg ccatggttta agaaacgata gctattgcac agtccttgag
     8701 ctagaattat aagtaattgt caacgattgt ttaggagaaa cttcatggga cttgcgaaac
     8761 gacatggcac gccactgctt ctgatcgtcc tcggcatcgg tattggcttt ttcatcgccg
     8821 gccaatgggt gccgaatgtg tcggcggttg gccgccagga ctacgagagc ctggaagcct
     8881 tcagcaatat tctgtcgatc gtcaagaaaa attacgttga ggatgtcgag accaaaaact
     8941 tggtcaacgg cgccatcaat ggcatgttga gctccctgga cccgcacagc gcctacctaa
     9001 cgcccgacct ctacaaggat ctgcaaagcg atacccaagg acgatttggc ggcttgggca
     9061 tcgagatcac cgtcaaaggc gggatgttga ctgtggtgtc gccgatcgaa gatactccgg
     9121 cgcacaaagc cggcataaag ccgggcgacc agatttttaa gatcgaagac gagttcacca
     9181 aagacatgtc gctggtggac gccgtgaaaa aaatgcgcgg gttgaagggt acgaagatca
     9241 atttaacaat ccggcgcgaa aacgccaccg atctcatcga cgtctctttg gtccgtgaca
     9301 tcattcgcgt gcagagcgtc aagagccgct cgttggagcc tggttacggc tatgtgcgtt
     9361 tggcgcagtt ccaagaacgc agcgaccgcg atgtccagcg cgccttggaa aaattggcgg
     9421 cggaggcgag cggtcttaag ggactggtgt tggatctccg caacaatccg ggcggcttgt
     9481 tgaaccaggc ggtccgagtc tcggacctgt ttctcgactc aggcatgatc gtttacaccg
     9541 acggccggat cgaatcgcaa aagcaaaaat tcttcgctca gaaggaaggc acctggatgg
     9601 atttcccgct ggtcgttctg gtcaatggcg gtagcgccag cgcttcggag atcgtcgccg
     9661 gcgcgttgca agatcacaaa cgcgcggtgg ttttgggcac caaatcattt ggcaagggct
     9721 cggtacagac gatcttgcca ttggacgata attcggcgct gcgcctgact acggcgcgtt
     9781 atttcacacc caaaggccgg tcgattcagg ccaccggcat cgtgccggat attctcattg
     9841 acaacatccc actgcaggat ggcaaacccg acgatagaaa gcggccgagt ttgcgcgagg
     9901 aaaacctgcc gggccacttg aataaccctc tgcaaaattc ggccaaagaa caagagcaga
     9961 tcgatcgcga acgcgaaaag cagattccca cctccggccc cacgggggat gagaccatcg
    10021 ataacgatca gcagctcaaa cgcgctctcg acctattgaa gagctgggat
//
LOCUS       DASQKS010000632         6648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4159076, whole genome shotgun sequence.
ACCESSION   DASQKS010000632 DASQKS010000000
VERSION     DASQKS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159076"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VE170_16390"
     CDS             complement(<1..737)
                     /locus_tag="VE170_16390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ17072.1"
                     /translation="MLPILFGLLTSLCFAVASLLAQRGYHIGPAPWGAWITIAANAAF
                     LLGGHLLIEGDTRIFIADNLIFVAVGLFVPGVTRVLSFRGIRTMGSSITSTIVNTTPM
                     FSTVLAMLILGERPSALVLGGVLLTVGGLITVSWGGEKASYSKIELVFPFLCALLFAM
                     KDISVRWGLSGGGGQPILAAGIAALTSTVEIFLVTRYGQGVKFALPRLPVLRWFVWSG
                     IFTGGAFLFMYIAFSLERVSIVAPLVN"
     gene            813..1859
                     /locus_tag="VE170_16395"
     CDS             813..1859
                     /locus_tag="VE170_16395"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYJ17073.1"
                     /translation="MAEVNLSLLRRRLLNWYRKNQRDLPWRRSRDPYAIWISETMLQQ
                     TQVKTVLPYYARFLDALPTVQALAGAPLPRVLRLWSGLGYYRRAENLHKAARQIVAHH
                     GGNLPREYQLLRALPGIGDYTAGALLSIAFDEKYPAVDGNVRRVFNRILNTTTEAAVR
                     ETAGKLAQTSQPGNLNQALMELGATVCTPKEPRCGDCPLTLDCRSRNRLRQSAPRPRA
                     YFRNVTWPLAIVDYHGKILLRRRQADGLLARLWELPGGEIAATATPEAAIRKELRPLA
                     VTRLRPQRLGELRHSITDRRIHAPVLLYRWPLKQSLPPGWRWIVPTQLHRYPTSSMTD
                     KAIKLFIEHEKSPG"
     gene            1840..2601
                     /gene="mtgA"
                     /locus_tag="VE170_16400"
     CDS             1840..2601
                     /gene="mtgA"
                     /locus_tag="VE170_16400"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HYJ17074.1"
                     /translation="MKRVLVSTLFATLLVGTGYYFYFSLPDVSALKQKNPSSTALMRL
                     RDQEYNKQNSRGRRQQIWVPYGAISEHIKKAVLISEDAGFYSHKGVDVNELKAAFKKD
                     LETLSFSRGGSTITMQLAKNLYLSPSKNPLRKLKEIIIAHQLEHALPKRRIFEVYLNV
                     VELGHNIYGVEAAARHYFGKSAGAVDLVEAATLAAMLPSPRNSRERSLAARRNIILNR
                     LASVGYLSQSENRDAQKLAPASKLKEAEIDPPPAE"
     gene            2717..2929
                     /locus_tag="VE170_16405"
     CDS             2717..2929
                     /locus_tag="VE170_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17075.1"
                     /translation="MEKPISRRIDTSLGDLIAAASEIAFEYSNNDKDAYNLAQLALLE
                     MIKKRSQPLDLDQELEILAPLSQLIH"
     gene            3171..4133
                     /gene="msrP"
                     /locus_tag="VE170_16410"
     CDS             3171..4133
                     /gene="msrP"
                     /locus_tag="VE170_16410"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HYJ17076.1"
                     /translation="MLIKKPADIKTGEITPKEHYLNRRQFMVSATAMALSSGAMLGGL
                     ELATESSIAYGAEKLANVKKSNFSTNEKLNTLKEITSYNNFYELGPDKEDPAENAKYL
                     TTRPWSVAVEGEVKKAKTYDIEELIKLAPLEERIYRLRCVEAWSMVIPWIGFPLSALI
                     KLAEPTGNAKFIQFVTLNDPKRLPGQKLPTLEWPYVEGLRMDEALHPLTILSVGLYGE
                     VLPAQNGAPIRLVVPWKYGFKSIKSIVKVRFVEKQPAASWNIMQPQEYGFYSNVNPEV
                     DHPRWTQASERRIGEFRRRKTLMFNGYGEQVASLYKGMDLKKYY"
     gene            4211..4834
                     /locus_tag="VE170_16415"
     CDS             4211..4834
                     /locus_tag="VE170_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HYJ17077.1"
                     /translation="MLVYLKPAAFIVCLIPLGQLGYRYYSDDLTANPIEYITRFTGSW
                     SLFILLASLTVTPLRRLTGWNEVIKLRRMLGLFAFSYAALHFSTYMVLDLFFDFSAIA
                     KDILKRPYITVGFSAFVLMIPLAVTSTTGMIRRLGKRWQQLHYLVYGIAILGVLHFYW
                     LVKADIRRPVQYGAVLTVLLGSRLAFKLAPVLKQKLSGKPAAVRSES"
     gene            5201..5818
                     /locus_tag="VE170_16420"
     CDS             5201..5818
                     /locus_tag="VE170_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ17078.1"
                     /translation="MKTGVLLVPSCLAALIYFSSCASWQVGSDVQSGRTALQTGRAND
                     AVGYLMRAADLDPTYRIPSRVGESVLTYLGRAYYETGRDKEALTTLEKAVSINKDDAL
                     AHLYLGLALLRTGDSDRGSKEIEAGLKSIHETIEYIAADPIHGLAWDSGRSIRRDIES
                     ALSNKSDNNQLVMAAQRIGKDFDEEIDRARRDESARRGGGDSGGN"
BASE COUNT         1560 a         1989 c         1749 g         1350 t
ORIGIN      
        1 ctgttcacca gcggcgcgac gatcgatacc cgctccaggc tgaaggcgat atacatgaat
       61 aaaaaagcgc caccggtaaa aattcccgac cagacgaacc agcgcaacac cggcaagcgc
      121 ggcagcgcga acttcacccc ctggccataa cgcgtaacca gaaaaatttc gacggtagaa
      181 gtcagcgccg cgattccagc ggcgaggatc ggctggccac cgccgccgct caagccccag
      241 cgcaccgaaa tatctttcat cgcgaacaac aatgcgcaga gaaacggaaa caccagttcg
      301 atctttgaat aggacgcttt ctcgccgccc caggacacgg tgatcaatcc gccgacggtc
      361 agcagcacac cgccgaggac caacgcgctc ggtcgctcgc caagtatgag catcgccaat
      421 acggtggaaa acatcggcgt ggtattgacg atggtggagg tgatcgagct gcccatggtg
      481 cggatgccgc gaaagctcag cacccgggtg actcccggga cgaacaggcc gacggcaacg
      541 aagatcaagt tgtcggctat gaaaattcgc gtgtcgccct cgatcagtaa atggccgccc
      601 aagagaaacg cggcgttggc ggcgatggtg atccacgcgc cccagggcgc cggcccgata
      661 tggtagccgc gttgcgccag caaactcgcc accgcgaagc aaagtgaagt taacagccca
      721 aataaaattg gcagcacggc gcgcagcgag ttcccttcaa agctgatttc atttacagtg
      781 cgttataact gaaaccccgc ccgactgcat tcatggctga agtaaatctt tctctgctgc
      841 ggcgccggct gctcaactgg tatcgaaaaa atcagcgcga tttaccctgg cgtcgttcgc
      901 gtgatcccta cgccatctgg atctcggaga ccatgctgca gcaaacccaa gtaaagaccg
      961 tgctgcccta ctacgcgcgc ttcttagacg cattgccgac cgtgcaagcc ctcgccgggg
     1021 cgccgctgcc gcgggtgctg cgcctgtggt caggactggg ctactaccgg cgcgccgaga
     1081 atttacacaa agccgcgcgc cagatcgtcg cccaccatgg cggcaatcta ccgcgtgagt
     1141 atcaactgct gcgagctctc ccaggtatcg gcgattacac cgcaggagcg ctgctcagca
     1201 tcgccttcga cgaaaaatat cccgccgtcg acggcaatgt acggcgcgtc tttaaccgga
     1261 tattgaacac gaccaccgaa gcggcggtgc gcgagaccgc cggcaaactg gcgcaaacct
     1321 cacaaccggg aaatctcaac caagccttga tggagctcgg cgccacggtc tgtacgccga
     1381 aagagccgcg ctgcggcgac tgtccgctca ctttggactg ccgcagccga aaccggctgc
     1441 gacaatccgc accgcggcca agagcctatt tccgcaacgt cacctggccg ctggccatcg
     1501 tcgactatca cggcaagata ctgctgcgac gccgccaagc cgatggcttg ctggctagat
     1561 tgtgggaact ccccggcggc gaaatcgccg ccacggcaac accagaggcg gcgattcgca
     1621 aggaactcag accgcttgcc gtcacccgtc tacggccgca gcgcttgggc gaactgcgcc
     1681 atagcatcac cgatcggcgc attcatgcgc cggtcttact ctaccgttgg ccgctgaagc
     1741 aaagcctgcc acccggctgg cgttggatcg tgccgaccca gctccatcgt tatcccacct
     1801 cgtcgatgac cgacaaggct atcaagttgt ttattgaaca tgaaaagagt cctggttagc
     1861 accctattcg cgactctcct ggtcggcacc ggttactatt tttacttttc gttgcctgac
     1921 gtttcggcgc tcaagcagaa gaatcctagt agcaccgcgc tcatgcgctt gcgcgaccaa
     1981 gagtacaaca agcaaaactc acggggacgg cgccaacaaa tctgggtgcc ctacggcgcg
     2041 atctcggagc atattaaaaa agccgtcctg atcagcgagg acgccgggtt ctacagtcac
     2101 aaaggcgttg acgtcaacga gcttaaggcc gcattcaaga aggatcttga aaccctgagc
     2161 tttagtcgcg gcggcagcac catcaccatg cagctggcca agaacctcta tctcagtccg
     2221 tcaaagaatc ctctgcgcaa attgaaggaa atcatcatcg ctcaccagct tgaacatgct
     2281 ctgccgaagc gccgcatttt cgaggtttac ttgaatgttg tcgaattggg ccacaatatt
     2341 tacggcgtcg aagcggcggc gcgccactat ttcggcaaat ccgccggcgc cgtcgatctg
     2401 gtcgaagccg cgactttggc cgccatgctg ccgagcccgc gcaactccag ggagcgcagc
     2461 ttggccgccc ggcgcaatat tatattgaac cgcctcgcca gtgtcggcta cctgagccag
     2521 agtgaaaatc gcgacgccca aaagctcgcg ccagccagta aacttaagga ggctgaaatc
     2581 gatccgcctc cggcagagta aacttggcgc gcccgccgca agcatccgcg cagattaaat
     2641 ttggcttaac cattagctac atcctttttt cgctgccatc gtctaactaa ccaagggcag
     2701 aaaggagccg ccaaacatgg aaaaacccat aagccgcaga atcgacacca gcttggggga
     2761 tttgatcgcc gccgccagtg aaatcgcatt cgaatactcc aacaacgata aagacgccta
     2821 caatctagcc cagctggctc tgttggagat gatcaagaaa cgcagccagc cactcgattt
     2881 agatcaggaa ttagaaatcc ttgcaccgct ctcccaactc atccactagt tgcctaccac
     2941 cgcgccgggg tcccacggac cccggccgcc ttttgtcgcc gcgacagctc gcgggaactt
     3001 tgcctgacag agcatcgttg taatgcaaat ctcagcacct gcatgctggt cgacaaaaag
     3061 ggctcaatcc gctacagcca tagtggcgaa ggcgcctacg gcaaaaccga gacgacgatc
     3121 aaacaactgt ttggcgaata tccgtgcgcc taaatcgagg aggagaatcc atgctcatca
     3181 agaagccagc cgatatcaaa acaggggaga tcacccctaa agagcactac ctgaaccgcc
     3241 gccagtttat ggtttcggca acggccatgg cgctcagcag cggcgccatg cttggcggct
     3301 tggagctggc cacagaatcg agcatcgcct atggcgccga gaaactcgcc aacgtcaaga
     3361 aaagtaattt cagcaccaat gaaaagctca acacgctgaa ggagatcacc agctacaata
     3421 atttctacga gctcggcccc gacaaagagg atccggcgga gaatgccaaa tatctaacca
     3481 cccgtccatg gtcggtcgct gtggaaggcg aggtcaaaaa agccaagacc tacgacatcg
     3541 aggagctgat caagttggca ccgttggaag agcggattta tcggctgcgc tgcgtcgaag
     3601 cctggtccat ggtaatcccg tggatcggct ttccactgag cgcattgatc aagctcgccg
     3661 aacccaccgg caacgccaag ttcattcagt tcgtcaccct caacgacccc aagcggctgc
     3721 cgggacaaaa gctgccgacg ttggaatggc cttatgtcga agggttgcgc atggatgaag
     3781 cgctgcatcc gctgacgatt ctttccgtgg gtctttacgg cgaagtactg ccggcgcaaa
     3841 acggcgcgcc catccgtctc gtcgtaccgt ggaagtacgg tttcaaaagc atcaagtcga
     3901 tcgtcaaggt gcgcttcgtc gagaaacaac cggcggcttc ctggaacatc atgcagccgc
     3961 aggagtatgg cttttactcc aacgtcaatc ccgaagtcga ccatccgcgc tggacccagg
     4021 caagcgaacg gcgtatcggc gaattccgcc ggcgcaaaac cttgatgttc aacggttacg
     4081 gcgagcaagt cgcaagcttg tacaaaggaa tggatctgaa gaaatattat tgagcaaacg
     4141 tgtaaaatgc caatccgctg gcgttctttg gtcctacttg tatcgattta attttatatt
     4201 tttatctccc atgcttgttt atctaaagcc cgcggcattc attgtctgcc tgattcccct
     4261 gggccagctt ggttaccgtt attattcgga cgatctgacc gccaacccga tcgaatacat
     4321 cacccgcttc accggttcgt ggtcgttatt tattttgctt gccagtctga ccgtcacgcc
     4381 gctgcgccgc ctcaccggct ggaacgaagt gatcaaactg cgccgcatgc tcggcctgtt
     4441 cgcattcagc tacgccgcgc tacatttctc gacttatatg gtgctcgatt tatttttcga
     4501 tttctccgct atcgccaaag atattctcaa gcgaccgtat atcactgtgg gcttcagcgc
     4561 ttttgtgctg atgattccgc tggccgtcac ctcaacgact ggaatgatcc gccgccttgg
     4621 caagcgctgg cagcaactgc actatctggt ttatggaatt gcgatcctcg gcgtactgca
     4681 tttctactgg ctggtcaaag ccgacatacg ccgtccggtg caatatggcg ccgtactcac
     4741 ggtgttattg ggctctcgtc tggcattcaa gctggcgccg gttctgaaac aaaagctctc
     4801 cggcaagcct gccgcggtgc ggtcggaaag ttgattcgtt caaatcgttc aagccgttca
     4861 atcgctgcgc tccgttcaaa tcgttaatcg gattcggacg gtctgtaggc tttgaacggg
     4921 gctgaaaagc tctaccggaa gtctcatgac cgtgatccga ggtttgtcgg accgccaaag
     4981 ctgcataact cagcggtcct gcacctgcgc attggtgcca agagacataa ccgtcgaagc
     5041 gtccatctcg agtatgaata cgaactcggc gcctactgtt gcttcgccta ctgttggcac
     5101 ctattgtcga tcacgagatt gccgatcgct gcatcttctg cggcgcgccg gcaggctacg
     5161 agtcccgaag gcggtagcca aaaacccagg aggatgtcac atgaagacag gtgtgttgtt
     5221 ggttccgagc tgtttggccg cgctaattta tttttccagc tgtgccagct ggcaagtcgg
     5281 cagcgatgtt cagtccgggc gtacggccct gcaaaccggc cgcgccaacg acgcggtggg
     5341 ttatctcatg cgcgccgctg acctcgaccc cacatatcgc atcccctcca gggtcgggga
     5401 aagtgtcctc acctatctag gccgagccta ctatgagacc ggcagagaca aggaagcgct
     5461 gactaccctg gaaaaagccg tgagcatcaa caaagacgac gctttggccc atctctatct
     5521 gggcttggcc ttgctacgca ccggcgacag tgatcgcggt tcaaaagaaa tcgaagccgg
     5581 tctcaagagc atccacgaaa ccatcgaata catcgcggcg gaccctatcc atggtctggc
     5641 ctgggatagc ggcaggtcga tacgtcgaga catcgaaagt gccctgtcca acaagtcgga
     5701 taacaaccag ctcgttatgg cggcacaacg aatcggcaaa gacttcgacg aggagatcga
     5761 ccgtgcccgt cgtgacgaat cggcgcgacg cggcggcggt gacagtggcg gcaactgatg
     5821 cggtcagcga ttggagggat aactcatcta tggccggcgt ccgtccgcac tctgggctga
     5881 gcctgcagta tggcaatttg tctcgacgag aacgaccgtt tttctcgtgt cggacggcag
     5941 acgcatgcgc ggggacttgc tattcttctg atttggtatt aactcgccga tagaatgact
     6001 caacgggttc gggacttctt ttttattctg ccgactgacg aattcaggct tttgaaagaa
     6061 tgtctacctc ggcactcgat cggtttcgac gcactcgatc tcgcccttcc tgtctccaac
     6121 ggaaagcgtg cctttacttg tagcgaaaag cagctgcaag atgttatcca atctgtcgta
     6181 cagaagtttc cactcggcaa agccctggca atcttgatcc agcaacaatg tcagcccata
     6241 tctaaatccc agaacacgaa gccaaagaaa gcgaaaaaag gctaacttga tatccgccgt
     6301 atcctcttgc aggtaccgtg cgccaagcaa gtgacgcaac gcggtctcgg gctgagccct
     6361 gctggcgaaa ggccagcccc atataattag attacaggtc ggtgatatct ccaggctagg
     6421 ctcctcgaca cggaaagaaa ccctcgccgg ttattccaag tcttctccaa accctctcag
     6481 cattccggac aaagctatag ttgtcgactc cacggttgac aactcgaacg gttatccgat
     6541 acttcgaatc tatggcaccg caattctttt cggtcaaaca ggcttacaat ttggccgtcg
     6601 gcaccgagtt aaccctgcgc ggctgggcgc gctcgcggcg cgactcca
//
LOCUS       DASQKS010000633         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3730397, whole genome shotgun sequence.
ACCESSION   DASQKS010000633 DASQKS010000000
VERSION     DASQKS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3730397"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..1527
                     /locus_tag="VE170_16425"
     CDS             460..1527
                     /locus_tag="VE170_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17079.1"
                     /translation="MKPRSTSVIIAALAMISASPVQAGGLKQVAEISIPGDPITDIGI
                     MDIDQTTGLGYLADKTNKSVVVFDTKTDKFVSRIPGFVGLLKDGNASGPNGVVVVKGG
                     AEVWVSDGDSTIKVIDAKSGRITATVATGGSKRANGMALGEDIGVVIVANSNDDPPFL
                     SLISTAPGNKILAKIPIPESGENLERSAYHAPSGMFYTAIPVLRGDTTKGLLAQTDAK
                     NGKLAKLHELRCYPHSLSIVSDSTIFLGCSSAHGPSPKPGGDMAIFDIAAGKVETFLT
                     GYGGNGGSTVNRKLGQYYHSTSSGTLMVVDTKMKTGLQQLPTSNGARSLAVSLANNRV
                     YVATTAKELACRGCIVVYAPE"
     gene            1825..2190
                     /locus_tag="VE170_16430"
     CDS             1825..2190
                     /locus_tag="VE170_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HYJ17080.1"
                     /translation="MAFRILADVTVVVHLGFVLFVVLGGVLVARWPRIAWLHLPAAAW
                     GAWVEFAGWLCPLTPLENWFRQQGGEAVYSVSFIERYLVPMLYPSSLTRALQWGLGGL
                     VLLINLTVYLLVFYRRARL"
     gene            2497..2982
                     /locus_tag="VE170_16435"
     CDS             2497..2982
                     /locus_tag="VE170_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17081.1"
                     /translation="MGEASSIIGFIIIVILYIVIGIMSAAGSIFISQRVFAPKAEQIF
                     YGILLIPVAGFYLAFTAYFGIETAWRLESAAVLAFVAIGLIGIRLPFAIIAGYPLHGL
                     WDLLHELNAHGGYSVFEPGQATAVPLAYGIFCAAYDLCMGAYFYTRRSVWGAARKTSM
                     R"
     gene            complement(3039..3467)
                     /locus_tag="VE170_16440"
     CDS             complement(3039..3467)
                     /locus_tag="VE170_16440"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ17082.1"
                     /translation="MKKTREAGDRMLKGGCFCGRIRYEAVGVPFHATNCHCSICRRTT
                     GASFVTWFSVARSQFRIVCGEPTRFRSTAQGARSFCPHCGTQLTFEHEDSLDEIGITT
                     CSLDDPNELPPKDHTWTGSKVDWVKLADQLPEYQDNRQKE"
     gene            3792..4061
                     /locus_tag="VE170_16445"
     CDS             3792..4061
                     /locus_tag="VE170_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HYJ17083.1"
                     /translation="MCRSIKTLFNFQPPVTEDEIRAASLQFVRKVSGFSKPSKPNEAA
                     FLAAVDEIAASAANLLSTLETNAPPKNRAEEAAKASARAAQRFPN"
     gene            complement(4144..5127)
                     /locus_tag="VE170_16450"
     CDS             complement(4144..5127)
                     /locus_tag="VE170_16450"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17084.1"
                     /translation="MLKITLVGEAIFRIFVAALLLSLSERGGEAAELKKLTVGYSTVG
                     PAGIGLWMAKEIGAFDKYGIDVQLIFVSSGPIVVQALLGGDMQAGLAATNAVTAAVLA
                     GAPLVSVMSLINRPYYRCWVQPEITRMEELRGKTLGVSRFGSVTDSLTRILLRRYHLE
                     GAVQIRQMGGTTEMAAAFQRRQIDGAVMATVRVNAPTRMLVDLEELGIRYSNVVIAAS
                     RDYHRRNPDIIEAMVRAYLEGVAAVSQQKEKALQVIQKYTRLQDPKLIQELYADGVRF
                     LERVPRLDAEALAPIVEFMGKKPIPVETVADNSIVNRLEREGFIDKLYGKR"
BASE COUNT         1185 a         1525 c         1461 g         1229 t
ORIGIN      
        1 ggtgtttggc taagccttgg catcattttc gtttaagcgc cgccatccgc ccattgccga
       61 cgtgctccgg ccgcgcaatt ttggcgcgcc gcatcgccat gccttagcgc gtccgtttca
      121 gtcagatgct cgaaagcatc gatgctgcga ttttcattag catcggcgat ttatttgacg
      181 actataaata tttgaaagct ctatgactta cgtcaacgta accgcggaac cttcggcagc
      241 tggaatagcg gcaatgattg gaaacaaacg gtgcgctttc gacgcgcatc tcaggccatt
      301 tcaagcgact aacattgatt tgagctaggc tgctatcgca gctttcgctg agagctcaac
      361 gggctttcat tacggctctc tctggtgtag ggatgcggcg atggtgtgac cgagataatg
      421 tgatcgcaag tcgccaagaa aagacagagg gaggagccga tgaagccgcg ttccacgtcc
      481 gtcatcattg cagcactcgc catgatctcc gctagtcccg tgcaggccgg aggcctgaaa
      541 caggtcgcag aaatatcgat acccggtgat ccgatcacag acatcggcat tatggacatc
      601 gaccaaacga cgggtcttgg ctatcttgcc gataagacca acaaatccgt tgtcgttttc
      661 gataccaaga ccgacaaatt cgtctctcgg ataccgggct tcgtcggact gctgaaggac
      721 ggcaacgcct cgggaccgaa tggtgttgtc gtcgtcaagg gtggcgccga agtatgggtg
      781 agcgacggcg acagcacgat caaggtgatc gatgccaaga gcggccggat cacggcgacg
      841 gtcgccaccg gcggctccaa gcgagccaac gggatggcct tgggcgaaga tatcggcgtc
      901 gtcatcgtcg ccaactcgaa cgacgatccg ccgttcctca gcctgatctc aactgcgccc
      961 gggaataaaa tcctggcgaa gatccctatt ccggaatccg gcgaaaacct ggagcgttcc
     1021 gcgtatcacg cgccaagcgg catgttctac acagccatcc cggtattacg cggggacacc
     1081 accaagggac tgttggccca gaccgatgcc aaaaacggca agctcgcgaa gctgcacgag
     1141 ctccggtgtt atccgcatag tctctcaatc gtctcggatt cgactatttt cctgggttgc
     1201 agcagcgccc acggcccgtc tcccaagccc ggcggtgaca tggcgatctt cgatatcgcg
     1261 gcgggcaagg tggaaacatt tctcacggga tacggcggca acggcggctc gacggtgaat
     1321 cgcaaactcg gccaatacta ccattccacc tcaagcggca ccttgatggt ggtcgacacc
     1381 aagatgaaaa cgggcctgca acagctaccg acgtcaaatg gcgcgcgctc gctcgcggtg
     1441 agtctcgcca acaatcgcgt ctatgtggcc acgacagcga aggagttggc ctgccgggga
     1501 tgcatcgtgg tgtacgcgcc ggagtaagga tttgaggttt ggcgtagccg gtggctgaaa
     1561 ctgataactg cgtttccgtt ggagcgccat gacaggatgt aacacagctg taacataaag
     1621 gcgctaaatt ggagtagtga aacaatacta cgattggatc gttgtcgcgg ttatcggcgc
     1681 tctcgctgta gttttatttg cttggatgac ccggtgatca gaaaaaattg taacgacagg
     1741 ctgtgaccct gtcagatccg ccggatatta aaatatttgc aaccaacgcg gccgccgagt
     1801 ccgtagtccc ggcgcctgcc cgtcatggct ttccgcatcc tcgccgacgt cacagtcgtc
     1861 gtccatctgg gcttcgtgct gttcgtggtc ctcggcggtg tgctggtcgc gcgctggcca
     1921 cggatcgctt ggctacatct tcccgcggcc gcttggggcg catgggtcga attcgcgggc
     1981 tggttatgtc cgctcacacc cctcgagaac tggtttcggc agcaaggcgg cgaagccgtc
     2041 tacagcgtca gcttcatcga gcgctatctc gttccgatgc tatatccgtc ctcgctcacg
     2101 cgcgcgctcc aatgggggtt gggcggccta gtgctcttga tcaacctgac cgtgtatctg
     2161 ttggtctttt accgtcgcgc tcggctgtag cttattcctg ggtgcggtgg agttaacatt
     2221 ttcgcttcag accgatcgct aagagcgacg gctaagcgtt gggctaaccc tgattgaacc
     2281 gatttctccg acggctcatt cctgcgggga tctggctgta gcgtcttaca gttcatcctg
     2341 tggcggttac cgaacttgac tctgatctaa tcaacgacat agccttcgag ccgtcaggga
     2401 gaaattccgc ccgcgttttt cgccgtgtta tgcgggtcac caatggtcag gtgagaacgg
     2461 gcgagatcac ataaaacaag aatggaagta agtcagatgg gtgaggcatc cagcattatt
     2521 ggatttatca tcatcgtgat cctctacata gtgattggga tcatgtcggc agctggttcc
     2581 atcttcatat ctcaaagagt gtttgcgccc aaagcagagc agattttcta cggaatattg
     2641 ctcattccgg tcgccgggtt ttatctggct ttcacggcct actttggaat cgaaacagcg
     2701 tggcgattgg aatcggcagc cgtattggca ttcgttgcga taggtttaat aggcatacga
     2761 ctaccattcg caataattgc cggctatccg ttgcatggac tctgggattt gttgcatgaa
     2821 cttaatgcac atggaggtta ctccgtattc gaaccaggtc aggcgactgc ggttccgctc
     2881 gcatacggta tcttttgtgc cgcctacgac ctctgcatgg gggcatattt ctatacacgt
     2941 cgcagtgttt ggggtgctgc tcggaaaacc agtatgaggt aaacccggga tcggattgtt
     3001 aaagatttga cagcggatct tctcgcttaa atttcttgct actctttttg tcgattgtcc
     3061 tggtattcgg gcaactgatc cgcgagttta acccaatcga ccttgctacc ggtccacgtg
     3121 tggtccttcg gcggcagttc gtttggatcg tcaaggctgc aagtcgtaat gccgatttcg
     3181 tccaaagaat cttcgtgctc aaaagtcagc tgcgtgccgc agtgcgggca gaaactccgc
     3241 gcgccctgag cagtagatct aaaccgcgta ggttcaccac agacgattcg gaactgcgat
     3301 cgagccacgc tgaaccatgt aacgaacgaa gcgccggtgg ttcgacgaca gatagaacaa
     3361 tggcagtttg tcgcatggaa gggaacgccg actgcctcat aacgaatccg tccgcaaaag
     3421 cacccgccct taagcattcg atcacccgcc tctctggttt tcttcatgac tggtttgttt
     3481 tcagaaaaaa cgatcaggta tcacgctaat agatttttac cgcgaccatc gccggcacag
     3541 gatactgcat tcttgcgccg cgatgtcatt cttggcctgg ccactcaaca gcccaatcct
     3601 cgactccata ctacacaaga ttgcgtttgc tggaacgtcg ctaagcaatt gtttacgcgg
     3661 ttgcaagaga gatcacgtcg cccaaataac caaacgcaga cccaatcctc gcacttaagt
     3721 ttcttgccgc agactgctac gctggtggca tcagctcaga gctacaactc tgagataccg
     3781 aggtaaagac tatgtgccgc agcatcaaaa cgcttttcaa tttccaaccg ccggtgacgg
     3841 aggacgagat tcgcgcggcc tcgttacaat ttgtcagaaa ggtgagcggc ttcagcaagc
     3901 catcgaaacc caacgaagcc gcgttcctag ccgccgttga cgaaatcgcg gcgagcgcag
     3961 ctaacctcct cagcaccctc gagaccaatg caccgccgaa gaaccgcgca gaagaggctg
     4021 ccaaggcgag tgcgcgcgct gctcagaggt ttcctaattg attggtcgcc actaacgttc
     4081 gacgttgtgg cgatataact ccgctccctg agcgatctca ggatcactgg caggaagttc
     4141 gcgttaacgt ttgccgtaga gcttgtcgat gaaaccttcg cgttccaggc ggttgacgat
     4201 gctattgtcg gcgaccgttt cgaccggaat cggcttcttt cccatgaact cgacaatcgg
     4261 cgcgagcgct tccgcgtcca gacgcggtac tctttcgaga aatctgactc cgtccgcgta
     4321 gagctcctgt atcaatttcg gatcttgcag tcgcgtgtac ttctgaatga cttggagcgc
     4381 tttctccttt tgctggctga ccgcggcgac gccttccagg taagctcgca ccatcgcctc
     4441 gatgatatct ggattgcgcc gatggtaatc gcgcgaagcc gcgatcacga cattcgaata
     4501 ccggatgccc aactcctcta gatcgacaag catgcgcgtc ggcgcattga cgcgcaccgt
     4561 cgccatcacg gcgccgtcga tttgccggcg ctggaacgcc gccgccatct ccgtcgtccc
     4621 gcccatctgg cggatttgaa ccgcgccctc gaggtggtat cggcgcagca gaatgcgggt
     4681 caggctgtcg gtcactgagc cgaagcgcga gacgccgaga gtttttccgc gcaattcctc
     4741 catccgggtg atctccggct gaacccagca acgataatag ggccgattga tcaagctcat
     4801 caccgacacc agcggcgcgc cggcgagcac cgccgcggtc acggcattgg tcgcggccaa
     4861 accggcttgc atgtccccgc ccaaaagcgc ctgcaccacg atcggtccgg aggaaacaaa
     4921 tatcagctgc acatctatgc cgtatttgtc gaacgcgccg atctccttcg ccatccacaa
     4981 tccgattcct gcaggtccga ccgtggaata gccgacagtc aatttcttca gctccgccgc
     5041 ttctccgccg cgctcggaaa gcgacagcag gagcgccgct acgaagatgc gaaaaatcgc
     5101 ttcacctacc agtgtaatct tcagcatgaa atctccaagt gcttttccag ccctttggcg
     5161 ccaaggtgcg tgaacgctaa tcaaaatcgt ggcttcaggc aagcaggatc gtcgcgatcg
     5221 taatcgacag aggcgtgttt atcgacaaat cgcttgggcc atcgagatca acgatcatgt
     5281 ggagaagctt ccatcgggat gatcgcgtca atcaaaaccg gctctagtca tcgctcacaa
     5341 gattgattga caacagatta atcaaaaggg taaatcctgc gcagctgagg atatttgaat
//
LOCUS       DASQKS010000634         8159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5811406, whole genome shotgun sequence.
ACCESSION   DASQKS010000634 DASQKS010000000
VERSION     DASQKS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5811406"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VE170_16455"
     CDS             <1..1239
                     /locus_tag="VE170_16455"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17085.1"
                     /translation="LEGWDQAAEFRVSKDGKHAYVSNYQGFSIVDVSDPAKPRVISKV
                     KNNAAVQSQYIDVIGNTLVINQDGLRDPKNPQWEGGIRLYDITDPAKPREVGFFKTDV
                     APRRGVHGFWLHEDPKQGKFAFIATTMEGYYDQILLIVDINDPANAKEVARWWFPGQW
                     TAGGEKPGDNWVEPDRGLREGLPKIWVALHDITTYKDRAYLAYRDEGVIILDISDVRK
                     PTKISQIKWSPPEEGNTHSIGIVVPSHGGRPDVIIAGDEITQPQQCPFGYMHVLDVRN
                     EKNPVQISTFRLPLNRFCPPDRPGRRFGIHDIERMIKGNIVYSAWENSGFWAVDISDP
                     HQPKAVGHFVPPVFRRVNSDAGHADDVFVHDNGLVFFSSSDPGGGFWITRYTPGVKGT
                     VSWTEDNKNVTVKYDKRPEN"
     gene            1217..1645
                     /locus_tag="VE170_16460"
     CDS             1217..1645
                     /locus_tag="VE170_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYJ17086.1"
                     /translation="MTSAQRIDLKIFFLLGLIGAFLPWPALAGHGVEDTVDNLTADRV
                     KQLLDAGEKLTFIDMRPVKEYQQTRLPGAKSLPIAEVANRFSEIPKTGRVILYCDCKT
                     YDVADRAVFLEYRGYRNIFIMPEGYAGWVKRGYPLEKTPR"
     gene            1681..2007
                     /locus_tag="VE170_16465"
     CDS             1681..2007
                     /locus_tag="VE170_16465"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HYJ17087.1"
                     /translation="MLLVPSSLWAHAYLVKSVPAQRATLYRTPAKIQLWFNERLEPRY
                     SSFTVSEAGGHSIETIKSAVDAGDAKLVAAALNPLPPGRYLVKFRVLSVDGHVVEDQF
                     PFTIKQ"
     gene            2004..4010
                     /locus_tag="VE170_16470"
     CDS             2004..4010
                     /locus_tag="VE170_16470"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HYJ17088.1"
                     /translation="MSELAALVRFVHLTAAILLAGGLGFRLLVAQPAFAQAKQSAAAL
                     EQLHWRAQLRSLHWCVALLCITLPLSLWLHAVTVSGSSLPALSTLFLLATETQFGRVW
                     LIRMALLIAIAVLVRAKSATNQWALVFTLSSCLLAGLAFAGHAAAADGAEFVLQVCAD
                     AVHLLAGGVWLGGLVPLALLLRQCNRKDDAANLIVAREATRRFSILALASVIVLIGTG
                     LYNAWNLVGGFAPLFGTHYGKLLLVKLGLLLPLLATGAVNLLRLKPTIVATGDRARET
                     GAALRSLRRNVIIEVFLGLAILLIVGHMGITPPARHVPPDWPLSFRWDWTVLEKAPQA
                     VAEVQRGAIWLAVGVVALSAGIFQRINRLLATIVGIGAIAYAINLVHQAVLIDAYPDT
                     YKRPAVAYQAISIANGAALYQESGCAACHGNGGHGDGPAAAEFDPPPPDLTGRHANAH
                     TAGDLYWWLSYGVKPASAMPGFSQSLSDEERWDVINYLRALASGERAKNLAPVIDDQP
                     TWVAPDFAYETNNGAARTLKDHRGNKIVLLIILNLQDTEARLRELGSLSALLQAAEVE
                     IVVVPNLIDRLFVDKQLPGLIVSEGIREITQTYKLFARSFAGDDAITATPHVEFLIDK
                     QGYIRARWLPAENAAWHKVDLLMQQIDALRKEKPTAPAPDDHVH"
     gene            complement(4183..4692)
                     /locus_tag="VE170_16475"
     CDS             complement(4183..4692)
                     /locus_tag="VE170_16475"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HYJ17089.1"
                     /translation="MDSQLRHQVEDFYYFEAELLDARKLREWFELLTEDIRYWMPLRH
                     NTLERPESVSDELSQPGDAYYFNDDWQSLKIRVERAYSKIAWAEVPPSRTRHLITNIR
                     IKKDDGEQIDAHSNFLVYRTRQESDKDIFVGTRQDSLRRAGASFKLARRTIILDQAVL
                     DAKNISVFL"
     gene            complement(4685..5968)
                     /locus_tag="VE170_16480"
     CDS             complement(4685..5968)
                     /locus_tag="VE170_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYJ17090.1"
                     /translation="MDIKSLVDPEGGLIDRRIFADREIYDLERERIFARCWLYLGHEC
                     EIPNPGDFVTRYMGEDPVILARDLKGQLRAYLNMCRHRGNRVCRADRGNTKLFQCSYH
                     GWSYTTDGKLALVPMVDAFGNLDRSQWGLASVAQIDSYKGLIFATFDEDAPTLRDYLG
                     DMAWYLDILLDRREGGTEVSGPQRWVVDANWKTAAENFGGDGYHIASTHGSARELGID
                     TTTSKTRQWNKGCQIACDNGHILVSWLTPPDSGPWFAQPDDQLAGYMKEHEVEIEKRL
                     GTKRARQIAPSAGTVFPNLSVHWLTRTIRVWQPRGPDKMEIWSWAIVDKAASPKIKDV
                     MRFVAQYRFSPSGVFEQDDMDNWIQVTGAARSLIGQRFPANYQMSGNEPPVEIDLPGR
                     VKSRFSDNNQLSLYMHWAKMLQAKNWREVASASNG"
     gene            6124..7656
                     /locus_tag="VE170_16485"
     CDS             6124..7656
                     /locus_tag="VE170_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17091.1"
                     /translation="MAAKQTNISRRRFLQHSTTFGLGAAIAPVLGQYALAASKERVVI
                     YQGVSLDSLHPYGYSGGGINGIWLHLIEPLIVMDFTRQKYVGMLADSWEFQGRRWIFQ
                     LRKNIRFHNGAPFTAKDVAYSIERMKTDKRSLQGGDILDLEVETPDDFTVVFNTKQPL
                     SLLLDRLDTRYIISKAAAEKFGDQADNYAIGTGPYKFVSWQRGGNLVLARNDDYWGAK
                     AEIKEVILKGVKEEAARVAGLLSGQADVISNLPIEEIDRVAKHPRVRVEKIPGFRMYF
                     VAMNVTHKPFDNKLVRQAVNHAIDPAPIMKHVFSGNGQVLNGPLAANMTGFDPAIKRY
                     AHDPKKARELLAQAGYPNGFEIKLHFSPDRHLKGREVCEVIGHQLAKVGIKIELVGQE
                     YAVYWGRDGVNGGKLPFYYAGRTASDADTFYDQYFHTGITKRIGYSNPEADKLIEEQQ
                     RTADQKKRVAILQQAGRLIMDDAPFVPLYTLSEIYGVAKNVNWKGTPDNRILAVEMKI
                     RG"
     gene            complement(7742..>8159)
                     /locus_tag="VE170_16490"
     CDS             complement(7742..>8159)
                     /locus_tag="VE170_16490"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HYJ17092.1"
                     /translation="GGGMFHYFDGAAQAPGLLQDQAQMGSALVRAYQATGDGEYADRA
                     RRLAEFTLAELTNPAGGFYDICAQESTALKLRLMLIEQNGAAAAFFLSLAQATKDARY
                     RGAALSGLRAFAGDFSQYGVHAAPFGLALGQLQRLE"
BASE COUNT         1696 a         2466 c         2317 g         1680 t
ORIGIN      
        1 ctcgagggtt gggaccaagc cgccgagttt cgcgtcagca aggatggcaa acacgcgtac
       61 gtctcgaact accagggttt cagcatcgtc gatgtcagcg acccggccaa gccgcgcgtc
      121 atctcgaaag tcaaaaacaa cgccgctgtg cagagccagt acatcgacgt gatcggcaat
      181 acgctggtga tcaaccaaga tggattgcgc gatcctaaaa acccccaatg ggagggcggc
      241 atccggctct acgatattac cgatccggcc aagccgcgcg aggtgggatt tttcaagacc
      301 gacgtagcgc cgcgccgcgg cgtgcatggt ttctggctgc atgaggaccc caagcaggga
      361 aagttcgcct tcatcgccac tactatggaa ggctactacg atcagatcct cttgatcgtc
      421 gacattaacg acccggccaa tgccaaagaa gtggcgcgct ggtggttccc cggccaatgg
      481 accgcgggcg gcgaaaagcc cggcgacaac tgggtcgagc ccgatcgcgg cctgcgcgaa
      541 gggctgccga aaatctgggt cgcgctgcac gacataacca cctacaaaga tcgcgcttat
      601 ctagcgtacc gcgatgaagg agtgatcatt cttgacatca gcgatgtgcg caaaccgacg
      661 aaaatcagcc agatcaagtg gtcgccgccc gaggaaggca atactcacag tatcggcatc
      721 gtcgttccat cccatggcgg caggcccgat gtcatcatcg ccggcgatga aatcactcaa
      781 ccgcagcaat gtccattcgg atatatgcat gttctcgacg tccgcaacga gaaaaatccg
      841 gtgcagattt cgaccttccg gctgccgctc aaccgctttt gcccgcctga ccggcccggc
      901 cggcgcttcg gcattcacga tatcgagcgc atgatcaagg gcaatattgt ttatagcgcc
      961 tgggagaatt ccggcttttg ggcggtcgac atttccgacc cgcatcaacc gaaggcggtc
     1021 ggccacttcg tgccgcccgt gtttcgccgg gtgaattccg acgccggcca tgccgatgat
     1081 gtgtttgtcc acgataatgg cttggttttc ttcagctcct ccgatcccgg cggcggcttt
     1141 tggatcacgc gctacacgcc aggcgtgaaa ggcacggtct cctggactga ggacaacaaa
     1201 aatgtgaccg ttaagtatga caagcgccca gagaattgac ctcaagatat ttttcctact
     1261 cggattgatc ggtgctttcc taccgtggcc ggcccttgcc ggccacggag tggaggacac
     1321 ggtcgacaac ctcactgccg accgggtgaa acaacttctc gatgccggtg aaaagctgac
     1381 attcatcgac atgcgtccgg tcaaagaata tcaacaaact cgcttgccgg gcgctaagtc
     1441 actgcccatc gccgaagtcg ccaaccgctt cagcgagatt cctaaaaccg gccgggtgat
     1501 tctttactgc gactgcaaaa cctacgacgt cgccgaccgc gcagtgttcc tggagtaccg
     1561 cggctatcgt aacatcttca tcatgcctga aggttacgcc ggctgggtga aacggggcta
     1621 tccactggag aaaacgccgc gctaatggtc aggttggctc tcgccgcact gcttgcaact
     1681 atgttgctcg ttccatcatc gttgtgggcg cacgcttacc tggtcaagtc ggtgccggcc
     1741 cagcgcgcca cgctgtatcg cacgccggcg aaaatccaat tatggttcaa cgaacgcttg
     1801 gagccgcgct actcgtcgtt caccgtcagt gaagccggcg gccattcgat cgagacgatt
     1861 aaaagcgccg tcgatgccgg tgacgcaaaa ctagttgccg ccgcgctgaa tccgcttcct
     1921 ccgggtcgat atctggttaa attccgcgtc ctgtccgtcg acggccatgt cgtcgaagat
     1981 cagtttccgt ttacgatcaa acaatgagcg aactcgccgc gctggttcgt ttcgtgcatt
     2041 taaccgcggc gatccttctc gccggcggtc ttggctttcg gctgttggtc gcgcagccgg
     2101 cttttgccca ggccaagcag tcggcggcgg cgctcgaaca actgcactgg cgcgcgcagc
     2161 tgcgcagcct tcattggtgc gttgctctac tctgcattac tttaccgctg agcctttggc
     2221 tgcatgccgt cactgtgagc ggctcatccc tgcccgctct cagcacgcta tttctccttg
     2281 ccacggaaac tcaattcggc agagtctggt tgatccgcat ggcgctgctg atcgccatcg
     2341 cggtattggt cagagcaaaa tcagcgacaa atcagtgggc gctggttttt acgctcagta
     2401 gctgtctatt ggctgggctg gccttcgccg gtcacgccgc ggccgccgac ggtgctgagt
     2461 ttgtcctgca ggtgtgcgcc gacgcagtac accttctcgc cggcggcgtc tggctgggcg
     2521 gcctcgtgcc attggctctg ctgctgcgcc aatgtaatcg caaggacgat gcggcgaatc
     2581 ttatcgtagc cagagaggcc acccggcgct tttcaatcct ggccttggcc agcgtaatcg
     2641 tgctcatcgg caccgggtta tacaacgcgt ggaatttggt cggcggtttc gcgccgctgt
     2701 ttggcacaca ttacggcaag ctcttgctgg tcaagctcgg tctgctgctc ccgttgctcg
     2761 ccaccggcgc ggtaaacctg ttgcggctca aaccgacaat cgtcgcaacc ggcgatcgcg
     2821 caagggaaac cggcgctgcg ctgcgaagcc tgcggcgcaa cgtcatcatc gaagtttttc
     2881 tcggcctggc cattttgctg atcgtcggac atatggggat cacgccgccg gcgcgccatg
     2941 ttccacccga ctggccgttg tcatttcgct gggattggac ggtgcttgaa aaagcgccgc
     3001 aagccgttgc cgaagtccaa cgcggcgcga tctggttagc cgttggcgtg gtcgcgctca
     3061 gcgccgggat ttttcagaga ataaaccggc tcttggcaac gatcgtcggc atcggcgcaa
     3121 tcgcgtacgc gatcaatctg gtacaccagg ctgtgctgat cgatgcttat cctgacacct
     3181 acaaacggcc ggccgtagct taccaggcga tctccatagc caacggcgcg gcgctctatc
     3241 aggagagcgg ttgtgctgcg tgtcacggca acggcggtca tggcgacggc cccgcggcag
     3301 ctgagttcga tccgccgccg cccgacctta ccggccgcca cgccaacgcc cacaccgccg
     3361 gcgatctgta ctggtggttg agctacggcg tcaaaccggc ttcggcgatg ccggggttca
     3421 gccaaagcct gagcgatgaa gagcgctggg atgtgatcaa ttatttacgc gcgctggcga
     3481 gcggcgaacg cgccaaaaac ctcgcgccgg tcatcgacga ccaacccaca tgggttgctc
     3541 cggattttgc ttatgagacc aacaacgggg cagccaggac tctcaaagac caccgcggca
     3601 acaaaattgt tttgctgatt attctcaatc ttcaggacac cgaagcacgc ttgcgcgaac
     3661 tcggcagtct ctctgctctg cttcaggctg cggaagtcga gatcgtcgtg gtgcccaact
     3721 tgatcgatcg cttattcgtc gacaagcagc tgcccggact gatagtgagc gaagggattc
     3781 gcgagatcac ccaaacctac aaactctttg cccgctcgtt cgcgggtgac gacgccatta
     3841 cggccacccc tcatgtcgaa ttcttgatcg acaagcaagg ttacatccgc gcgcgttggt
     3901 tgccggccga gaatgccgct tggcacaaag tcgatttact gatgcagcaa attgatgcgc
     3961 tgcgaaagga aaagcctacc gcgccggcgc ccgatgatca tgtgcattag cgcgcgttgc
     4021 gacctaccta gcaaattctc aatggacggg taaatactta tcgccggttc taatgcgggg
     4081 cctgtttcct gcgagtaagc ttgccgcttg gccgatggca ggtcatgcag cggccgtgaa
     4141 acttaagggc gcgctcaaac gtccggatcc gtgaaccggg gactatagaa acacactgat
     4201 attcttcgcg tcgagcaccg cctggtcgag aatgatcgtg cgccgggcaa gcttgaaact
     4261 tgccccggcg cgccgcaggc tatcctggcg cgtgccgacg aagatatctt tgtcgctttc
     4321 ctggcgggtg cggtagacca ggaaattcga atgggcgtcg atttgctcgc cgtcgtcttt
     4381 tttgatgcgg atgttggtaa tcaggtggcg cgtgcgcgac ggcggcactt cagcccaggc
     4441 gattttcgaa taggcgcgct cgacgcggat cttcaaactc tgccaatcgt cgttgaaata
     4501 ataggcgtcg ccgggctggg acagttcgtc gctgacgctt tcaggccgct ccagggtgtt
     4561 gtgccgtagc ggcatccagt aacgaatgtc ttccgtcagc agctcgaacc actcgcgcag
     4621 cttgcgcgcg tcgagcagct cggcctcgaa gtaataaaaa tcctcgactt gatggcgcag
     4681 ctggctatcc attggacgcg ctggcaacct cgcgccaatt ttttgcttgc aacattttgg
     4741 cccagtgcat gtacaggctc aactggttgt tgtcgctgaa gcggcttttc acccggccgg
     4801 gcaggtcgat ttccaccggc ggctcattgc cgctcatctg atagttggcc ggaaaacgct
     4861 ggccgatcag gctgcgcgcc gcgccggtga cttgaatcca attgtccatg tcgtcctgct
     4921 cgaaaacccc cgacggactg aaacggtact gcgccacgaa gcgcatgaca tctttgattt
     4981 ttggcgaagc cgctttgtcg acgatcgccc aactccagat ctccatcttg tccggcccgc
     5041 gcggctgcca gacgcgaatc gtccgcgtca gccagtgcac cgagagattg ggaaaaaccg
     5101 ttcccgccga cggggcaatc tgccgcgccc gcttggttcc taaccgcttt tcgatttcaa
     5161 cttcatgctc tttcatgtag ccggccagct gatcatccgg ctgagcaaac cacggcccac
     5221 tgtccggcgg cgtcagccag gagactagaa tatggccgtt gtcgcaggcg atctgacagc
     5281 ccttgttcca ttgccgggtt ttgctggtgg tcgtatcgat gcccagttcg cgcgccgaac
     5341 catgggtcga ggcgatgtgg taaccgtcgc cgccaaaatt ttccgcggcg gttttccaat
     5401 tggcatccac cacccagcgt tgcggtccgc tcacttccgt gccgccctcg cggcggtcga
     5461 gcaggatatc gagataccag gccatgtccc ccagataatc gcgcagggtt ggtgcgtcct
     5521 cgtcgaaggt cgcgaagatc agccccttgt aactgtcgat ttgcgccacc gaagccaagc
     5581 cccactgcga gcggtcgaga ttgccaaatg cgtcgaccat gggcaccagc gcaagtttgc
     5641 catcggtggt gtacgaccag ccatgatacg agcattgaaa tagtttggta ttgccccggt
     5701 cggcgcggca cacccgattg ccgcgatggc ggcacatgtt taaataagcg cgcagttgac
     5761 cctttaagtc gcgcgctagg atcaccgggt cttcgcccat gtagcgggtg acgaaatcgc
     5821 cggggttggg aatctcacac tcgtggccaa gataaagcca gcagcgggca aatatccgct
     5881 cgcgttccaa atcgtagatc tcccggtcgg cgaagatccg tcgatcgatg agaccgcctt
     5941 ctgggtccac cagtgatttg atgtccatgc caatacctcg cctaagccga gagcgcctgt
     6001 tcggtcatca taattctggc gccattgtgt caagttgggg ccgatttacc aagccgccgg
     6061 aacaaaaaat agttgcgcca gctggccact ccagtgctat aagcattgga aaggagcact
     6121 gccatggctg caaaacaaac caacataagt cgccggcgct ttcttcaaca cagtacgacc
     6181 ttcggccttg gggctgcaat cgcgccagtc cttggacagt atgcgctggc ggcgtccaaa
     6241 gaacgggtcg ttatttatca aggtgtcagc ctcgactcgc tccatcccta cggttacagc
     6301 ggcggcggca tcaacggcat ctggctccac ctgatcgagc cgctgatcgt gatggatttc
     6361 acgcggcaaa aatatgtcgg catgcttgcc gactcatggg aattccaagg acggcgctgg
     6421 atattccagc tcagaaaaaa cattcgcttt cacaatggcg cgccgttcac agcgaaagac
     6481 gtggcctatt ccatcgagcg catgaaaacc gacaagcgca gcctgcaggg cggcgacatc
     6541 ctggatttag aagtggaaac gcccgacgat ttcaccgtgg ttttcaacac caagcagccg
     6601 ctctcgctgc tgctcgaccg cctggacaca cgctatatca tcagcaaagc ggccgcggaa
     6661 aaattcggcg accaggccga caactacgcc atcggcaccg gcccctataa atttgtcagc
     6721 tggcagcgcg gcggcaactt agtcctggcg cgcaacgacg actattgggg cgccaaggcg
     6781 gagatcaagg aagttatttt gaaaggcgtc aaagaagagg cggcgcgggt cgctggctta
     6841 ctttcaggcc aggccgacgt catcagcaat ctacccatcg aagaaatcga tcgagtagcg
     6901 aaacatccac gcgtgcgtgt cgagaagatt cccggttttc gcatgtattt cgtcgccatg
     6961 aacgtcaccc acaaaccctt tgacaacaag ttggtgcgac aagcggtcaa ccacgccatc
     7021 gatcccgcgc cgatcatgaa acatgtcttt tccggcaacg gccaggtgct caatgggccg
     7081 ctggcggcga acatgaccgg cttcgacccg gcgataaaac gctatgccca cgatcccaag
     7141 aaagcccgcg agctgctcgc ccaggctggc tatccgaacg gcttcgaaat caaactgcat
     7201 ttctcgcctg accgccatct caaggggcga gaagtctgtg aagtgatcgg acatcaattg
     7261 gcaaaagtcg gcatcaaaat cgaattggtc ggtcaggagt acgcggtcta ctggggcagg
     7321 gatggcgtca atggcggcaa gctgcccttt tactacgccg gccgcaccgc ctccgacgcc
     7381 gacacgttct acgatcaata ctttcacacc ggcatcacca agcgcatcgg ctacagcaac
     7441 ccggaggccg acaagctcat cgaagagcaa cagcgcaccg ccgatcagaa aaaacgggtg
     7501 gcgatcttgc agcaagctgg ccgcctgatc atggacgacg cgcccttcgt gccgctctac
     7561 acgctatcgg aaatttacgg cgtggcaaaa aatgtcaact ggaaaggcac acccgacaac
     7621 cgcatcctcg cggtggaaat gaagatcagg ggatgagggg ggattggagt tgttggagta
     7681 atggagtact ggagtggtgg gttcttcggt gctcccaaca ctccattact ccatcattcc
     7741 attactccag cctttggagt tgtcccaacg cgaggccgaa cggcgcggcg tgaacgccgt
     7801 actggctaaa gtcaccggcg aatgcgcgca gccccgacag cgccgcgccg cgatacctgg
     7861 cgtccttcgt cgcttgagcc aacgagagaa agaacgcggc ggcggcgccg ttttgttcga
     7921 tcaacatgag gcgaagcttg agcgcggtcg attcctgcgc gcaaatatcg tagaaaccgc
     7981 cggccggatt tgtcagctct gctaaagtga actcagccaa ccgccgcgcg cgatcggcgt
     8041 attcaccatc gcccgtcgct tggtaggcgc gcaccaacgc gctgcccatc tgtgcttgat
     8101 cctgaagtag ccccggcgcc tgcgccgcgc cgtcgaagta gtgaaacatc ccgccgccg
//
LOCUS       DASQKS010000635         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3731166, whole genome shotgun sequence.
ACCESSION   DASQKS010000635 DASQKS010000000
VERSION     DASQKS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731166"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VE170_16495"
     CDS             complement(<1..195)
                     /locus_tag="VE170_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17093.1"
                     /translation="MPRTNTAWRRRIFIMALCVASGAAGAPLLQAAAAPTRVTLAYPS
                     PSPRVAPLWVAQDLDFFGKYG"
     gene            complement(219..719)
                     /locus_tag="VE170_16500"
     CDS             complement(219..719)
                     /locus_tag="VE170_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17094.1"
                     /translation="MSITLESFASDVQRILKADPSRAGREKVRDLVQEALKDEKFIST
                     YINDDTPDRQVIYEDADLGFCVCAHVNREAREANPHDHGSSWAIYGQAWGETQMSDWE
                     VLEAAADGQPGKVKRGQVYMLKPGMAHIYNEGDVHSPKRVAATGLLRIEGKNTTKLKR
                     FPYKAV"
     gene            838..1140
                     /locus_tag="VE170_16505"
     CDS             838..1140
                     /locus_tag="VE170_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17095.1"
                     /translation="MRRSFVEVHGSALAFGLESTARWVVGRSAVPSDPYDPRRIPQGA
                     RFHDKRTFLGEICCRTMSLLSSDAKYERWPECIALVQSNGAKVGQCGSVPGSTPRS"
     gene            1163..2110
                     /locus_tag="VE170_16510"
     CDS             1163..2110
                     /locus_tag="VE170_16510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ17096.1"
                     /translation="MDYTTLGRSGLKVSVAGLGCGGPSRLGLKDKTRSESDVVAHVRG
                     AIDLGINFFDTAEWYGTEVVVGKAIAGVARDRLVISTKKNIVPEDHGDPDNEIRRALE
                     QSLKNLGTDYVDVYHVHGAEPKDYANATARHLPTLLRLREEGKIRAVGITERFVVDPS
                     HQMAEGAVRDHVWDVIMVGFNLLNPGARRNIFPLTAANGVGVLISYALRRALSQPERL
                     KKLCAELVGTGAIPAGAVNLAAPLDFVTDSGAAVSIQDAAYRFCRHEPGVDVVLTGTG
                     NPQHLKANVESLARPPLPAPVQQRLKELFGNIDGLTGNR"
     gene            2136..3161
                     /locus_tag="VE170_16515"
     CDS             2136..3161
                     /locus_tag="VE170_16515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ17097.1"
                     /translation="MASNRGILTILLTVMMLVWPIGASAQESFFKGKTVRIVVGFAAG
                     GGFDTYTRAIARYWGRHIPGNPSIIVENMAGAGSLIAANHVYKAARPDGLTIGNFGGG
                     LFMQQLKGGAGIEFDARKFEFLGVPVQDNRSCAFTKASGITSMERWMAAKTPVKVGAT
                     APGDLVHDGPKILQAALGLPIHLVSGYKGTADIRLGAESGELAGGCWGWDSIKATWSR
                     ALESGEAIVVVQMVSKPHPDLPKVPLAISYAKSDDARQLIESGVTIPAITNRPYVVGP
                     GTPKDRVQLLRKAFDATMKDPEFVADAKKSRIDLEPIGGAEVQKMVADMFNLSPALIA
                     KLRDALK"
BASE COUNT          628 a          892 c         1031 g          681 t
ORIGIN      
        1 tccatatttg ccgaagaagt ccaaatcctg ggcgacccac aacggcgcaa cccgcgggct
       61 gggcgacggg taagcgagag tgacgcgggt gggcgcggcg gcggcttgga gaaggggtgc
      121 gcccgcggcg ccgctcgcca cgcaaagcgc catgatgaag atgcggcgcc gccaagcagt
      181 gttggtgcga ggcataacgt ttggcctcga gttggcggct agaccgcttt ataagggaat
      241 cgtttcaact tggtcgtgtt cttaccctct atgcgcaata agccggtggc cgcaacgcgc
      301 ttgggcgaat ggacgtcgcc ttcgttgtaa atatgcgcca tccccggttt gagcatgtag
      361 acttgaccgc gtttcacttt tcccggttgg ccgtcggcgg cggcttcgag cacttcccag
      421 tcgctcatct gcgtctcacc ccaggcctga ccgtagatcg cccacgaact gccgtgatcg
      481 tgcggattag cctcgcgcgc ttcgcgattg acatgagcgc agacgcagaa gcccaggtcg
      541 gcatcttcat agatcacttg gcggtcgggg gtgtcgtcgt tgatgtaagt cgagatgaac
      601 ttttcgtctt tgagcgcttc ctgtaccagg tcgcgaactt tctcccggcc ggctcgactc
      661 gggtcggctt tgagaatgcg ctgcacatcg gacgcgaacg attcgagcgt gatactcatg
      721 attcatcccc ctattttcaa ttgtgccgct gatcacccat gcattaacaa agtagtgtgg
      781 ctaccgtcaa tcgctatccg attgactgct ctgcgaccca gcactataag cgcaggcatg
      841 cggcgatctt tcgtcgaggt gcacgggtca gcactagcct ttggtttaga gtcgactgcc
      901 cggtgggtgg tcggccgttc ggcggtgccg agcgatccct atgacccacg gcggattcca
      961 cagggtgcca ggtttcacga taagcgcaca tttctcggcg aaatctgttg ccggacgatg
     1021 tcgctgttgt ccagcgatgc taaatatgag cggtggccgg agtgcatcgc ccttgtccag
     1081 tccaatggcg ccaaggtcgg gcaatgcggt tcggttcccg gcagcacacc tcggtcgtga
     1141 aaacgcaagg gaaagctgtt gaatggatta cacaactctc ggccgcagtg gtttgaaagt
     1201 cagcgttgcc ggacttggct gcggcgggcc gagccggttg ggattgaaag acaagacgcg
     1261 cagcgaaagc gatgtggtgg cgcatgtgcg cggcgccatc gatcttggca tcaatttttt
     1321 tgacacggcg gagtggtatg gcaccgaagt tgtggtcggc aaggcgattg ccggcgtggc
     1381 gcgcgaccgg ctggtgattt ccaccaagaa gaacatcgtg ccggaagatc atggcgatcc
     1441 cgataacgag attcgtcgcg cactcgaaca gagcctgaaa aatcttggca ccgattatgt
     1501 cgacgtctac catgtccatg gcgctgagcc gaaggattat gccaatgcca ccgcgcgtca
     1561 tttgccgacc ttgctgcggc tgcgcgaaga ggggaaaatc cgcgccgtgg ggattaccga
     1621 acggtttgtc gtcgacccgt cgcatcaaat ggcggaaggc gcggtacgtg accatgtttg
     1681 ggatgtcatc atggtgggat tcaatctgct caatcccggc gcgcggcgaa atatttttcc
     1741 gttgaccgca gccaacggtg tcggcgtgct gatcagttat gctttgcgcc gcgccttaag
     1801 ccagcccgag cgcttgaaaa aactctgcgc cgagcttgtc ggcacaggcg cgattcctgc
     1861 cggcgcggtg aatctcgcgg cgccgttgga cttcgttacc gatagcggcg cggcggtttc
     1921 aattcaggac gcggcctacc ggttttgccg tcacgagcct ggagtcgatg tcgttttgac
     1981 cggtacgggt aatccgcaac atctcaaagc caatgtcgag tcgcttgcgc ggccgccgtt
     2041 gccggcgccg gtgcaacaac gtttgaagga actgttcggc aacatcgacg gtttgaccgg
     2101 caatcggtag ttgggtggat aaacggagga atagtatggc ttcgaaccgt ggtattttaa
     2161 ccatattgct gaccgtgatg atgcttgttt ggcccatcgg cgcatcggcg caggagtcat
     2221 ttttcaaggg taagaccgtg cgcatcgtcg tcggattcgc ggccggcggc ggcttcgata
     2281 cctatacccg ggcgatcgcg cgttattggg gccggcatat tcccggcaat ccatcgatca
     2341 tcgtcgaaaa tatggccggc gccggcagtc tgatcgcggc caatcacgtt tacaaagcgg
     2401 cgcggccgga cggtctgacc atcggcaatt ttggcggcgg cctgttcatg cagcaactca
     2461 agggcggcgc cggcatcgag ttcgatgcgc gcaagttcga gttcctgggc gttccggttc
     2521 aggacaatcg ctcctgcgca ttcaccaaag ccagcggtat caccagcatg gaacgctgga
     2581 tggcggccaa gacaccggtg aaagtcggcg ccactgcgcc gggggatttg gtgcatgacg
     2641 gtccgaagat tttgcaagcc gcgctcgggc tgccgattca cctggtctcg ggttataagg
     2701 gcacggcgga tattcgcctc ggcgccgagt cgggggagct ggcgggcggc tgttggggct
     2761 gggactcgat caaagcgact tggtcgcggg ccctcgaaag cggcgaggcg atcgtggtgg
     2821 tgcagatggt ttcgaagccg catcccgatc tcccgaaggt gccgctggcg atcagctacg
     2881 caaaatcgga cgacgcccgc caactcatcg agtcgggtgt gacgataccg gcgattacca
     2941 atcggcctta cgtcgttggc ccggggacgc cgaaggaccg agtgcaattg ttgcgcaaag
     3001 cgttcgacgc gaccatgaaa gatcccgagt ttgtcgccga cgcgaaaaaa tcgcgtatcg
     3061 atctggaacc gatcggcggc gcagaggtgc agaaaatggt ggccgatatg tttaacctat
     3121 cgccggcgct gatcgccaag ttgcgcgacg cgctcaagtg aggacggggc attgcagact
     3181 tcatatggca aaatacccag agccgatggc aggcagctgt gagttgtagt ag
//
LOCUS       DASQKS010000636         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4971813, whole genome shotgun sequence.
ACCESSION   DASQKS010000636 DASQKS010000000
VERSION     DASQKS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4971813"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..571)
                     /locus_tag="VE170_16520"
     CDS             complement(59..571)
                     /locus_tag="VE170_16520"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HYJ17098.1"
                     /translation="MNQYGKPFLTADWRYPALLNYESDPALLQPMVPAGMELDTWNGK
                     TFVSMVGFSFLNTRVLGLAIPLHGNCEEINLHFYMRHKAEDGWRRGVVFIKEIVPRAA
                     IAWRETAARWSIVSIIGSGVSGRRAERAWRAMSRSFTRAVRRGFARHAVVGVSRRRAG
                     DNYLSRSEAV"
     gene            complement(600..938)
                     /locus_tag="VE170_16525"
     CDS             complement(600..938)
                     /locus_tag="VE170_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ17099.1"
                     /translation="MAAPKKTATLHVRRGVPGEAPRYDDFPVPYEEGMSVLDALRWIR
                     GNLDSSLAIRYSCINANACKTCMALVNGAVEYTCIAKLTPDTTTVEPLPKRPLIRDLV
                     TDVIPDDEKF"
     gene            complement(941..2635)
                     /locus_tag="VE170_16530"
     CDS             complement(941..2635)
                     /locus_tag="VE170_16530"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ17100.1"
                     /translation="MADLSTDVLIVGAGGAGMYAAIAAARGGASVLLLDKSQIGRGGA
                     TIMAQMTVAVALGEQEADSVQLHFQDTLNAGRGLCNQALAYLLCEDGPKRIREMAQWG
                     TRWATENGHIKQEMAPGHSVKRCCYVDFLNTGPAVAATLRRKVSETAAIRRISNLAAV
                     EIVVDEGRAMGAVALNLADGQWLIIEAKAVILAAGGLTKLYERNSASANMGGEAHAMA
                     LWAGAELIDMEFVQFFPIGHLAPRLVGMDPIMWDPFRYKLGGKLLNGRFEEFIDRYGS
                     TDFGRYNATRDVAAYAILKEVEAGRGTPHGGAYLDFRHIAEAELRGAFGPVIDRLLTN
                     GIDLTQTAVEVGPMAHYQMGGIRVDADMQTGIAGLYAAGEAVGGANGANRLSGNAITE
                     AFVFGERAGSSAALNLQKINSHRREAAAAAALERLRKSPCADAGAAPTTTAVALQAEL
                     QTLMWENAGPIRRGEKLSRALARIEGMRQQLPSLKIAAAESYNLDQQDWFELRAMLTT
                     AAAIVTCALARTESRGAHQREDFPAPNDALLKNQIVGWHNGELRTSWIAPVGLPGA"
BASE COUNT          536 a          918 c          880 g          593 t
ORIGIN      
        1 taagctttga taatcggcgg taattgcagc gagtgaaact agtcagtgag tcggaatctt
       61 aaacagcttc cgacctcgat agataattat ctccggccct ccggcgagaa acgccgacga
      121 cggcgtgccg cgcaaaacct cggcgaaccg ctcgcgtaaa gcttcgcgac atcgccctcc
      181 aagcgcgctc tgcgcgcctg ccagatacgc cactcccgat gatcgacacg atactccatc
      241 gtgccgccgt ttctctccag gcgatcgccg cccgcggcac gatctccttg atgaacacca
      301 caccgcgacg ccagccatcc tcagccttgt ggcgcatgta aaagtgcaga ttgatctctt
      361 cgcagttccc gtgcagcgga atcgctagcc ccaagaccct ggtgttgaga aacgaaaatc
      421 cgaccatgct gacgaaagtt ttgccgttcc aggtatccaa ctccatgccc gccggcacca
      481 tcggttggag cagcgcagga tcgctttcat aattgagcag cgccggatag cgccagtcgg
      541 cggtgagaaa cggtttgccg tactgattca tggagccact gtctcacaca attccacggt
      601 tagaattttt cgtcgtccgg gatcacgtcg gtcaccagat cgcgaatcag cggccgcttg
      661 ggcaggggct cgactgtggt tgtatccggg gtcaatttgg cgatgcaagt gtactcgacg
      721 gcgccgttga ccagcgccat gcaggtcttg caggcattgg cgttgatgca gctgtagcgg
      781 atcgccaggc tgctgtctaa attgccacga atccagcgca gggcatcgag caccgacatg
      841 ccctcttcat agggcacggg gaaatcgtcg tagcgcggcg cctcgcccgg gacgccgcgc
      901 cggacgtgaa gggtggcggt tttttttggc gccgccatgc tcatgcgccg ggcaaaccta
      961 caggcgcaat ccagctcgtg cgcagttcgc cgttgtgcca gccaacgatt tggtttttca
     1021 gcaatgcatc gttcggcgcg ggaaaatctt cgcgctggtg cgcgccccgg ctctcggtgc
     1081 gagcgagcgc gcaggtaacg atcgccgcgg cggtggtcag catggcgcgc agttcgaacc
     1141 agtcttgctg atccaaattg tagctctccg ccgctgcaat cttcaggctc ggcaattgct
     1201 gccgcatgcc ctcgatgcgt gccagtgccc ttgacaattt ttcgccgcgg cgaatcggcc
     1261 cggcgttctc ccacatgagt gtttgcagct cggcctggag cgcgaccgcc gtggttgtcg
     1321 gcgcggcgcc ggcgtcggcg cagggtgatt tgcgtaaacg ttcgagcgcc gccgcagccg
     1381 cggcctcacg ccgatgagaa tttatttttt gaagattgag cgcggcgctc gatccggcgc
     1441 gctcgccgaa gacaaaggct tcggtgatgg cgttgccgga caagcgattg gcgccgttgg
     1501 cgccgcccac cgcttcgccg gcggcgtaaa gcccggcgat gcccgtttgc atgtcggcgt
     1561 cgacgcgaat accgcccatc tggtaatgcg ccatgggacc gacctccacc gctgtttggg
     1621 tcagatcgat gccattggtc agcaggcggt cgatcaccgg accgaaggcg ccgcgcagtt
     1681 cggcctcggc aatatggcgg aagtccaggt aagccccgcc atggggcgtg ccgcggccgg
     1741 cctcgacctc tttcaagatc gcgtaggcgg cgacatcgcg ggtggcgttg taccggccga
     1801 aatccgtgct gccgtaacgg tcgataaatt cctcgaacct gccgttgagt aacttgccgc
     1861 cgagcttata gcgaaacgga tcccacatga tcgggtccat gccgaccagg cgcggcgcca
     1921 gatgaccgat cggaaagaac tggacaaact ccatatcgat caactccgcg ccggcccaca
     1981 aggccatcgc atgggcttcg ccgcccatgt tggccgacgc gctattgcgc tcgtagagtt
     2041 tggtcaaacc gccggcggcg aggatcaccg ccttggcttc gatgatgagc cactgtccgt
     2101 cggcgagatt gagggcgacg gcacccatcg ccctgccctc gtcgacgaca atttccaccg
     2161 ctgccaggtt gctgatgcgc cgaatcgcgg cagtctcgct gaccttgcgc cgcaaagttg
     2221 ccgccaccgc gggaccggta ttgagaaagt cgacatagca gcagcgcttg acgctatggc
     2281 cgggcgccat ctcctgcttg atatggccgt tctccgtcgc ccagcgggtg ccccactgcg
     2341 ccatctcgcg gatgcgcttg ggcccgtctt cacagagtag ataggccagc gcttgattgc
     2401 acaggccgcg tccggcgttc aacgtgtctt gaaaatgcag ttggaccgag tcggcctcct
     2461 gctcgccgag agcgacggca acggtcatct gcgccatgat cgtcgccccg ccgcggccta
     2521 tctggctttt gtcgagcagc agcaccgacg cgccgccccg cgccgccgcg atagcggcgt
     2581 acatgccggc gccgccggcg ccgacgatga gaacatcggt ggagagatca gccattggcg
     2641 attagccggc gcgattgagt tccgttccta tggtacgcgt gatcgatcgt agggaagttg
     2701 cttgcgtcga ctcgaactcg actttctctt ggtcgtcatt cccgtgccaa cgggaatcca
     2761 ggtttgttct gcgcgaatta gcctggatac ccgcgtccgc gggtatgacg gaaaccagtg
     2821 acttcttgag tgtggagacc ctccccttac atgtgttctc taaggagagt ccatgattct
     2881 gtgcttgcct acagagcatt aaaatagttt cgcatttcca gcacatg
//
LOCUS       DASQKS010000637         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3733486, whole genome shotgun sequence.
ACCESSION   DASQKS010000637 DASQKS010000000
VERSION     DASQKS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733486"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VE170_16535"
     CDS             <1..510
                     /locus_tag="VE170_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17101.1"
                     /translation="IQTGGMPEGLAALTAGALDAVVLSPPSNFRAVQAGMHELEDVGQ
                     LKIVFPNTPLSATRTYLRANRDTGLRFMRGFAQGLQRLRTDKEFSMKVLSKYTKVTDS
                     ETLGQLHQTYGVRYSGDRIPYVRPESIEEILRRTPGKEARDAKAADFIDNSLLQELEK
                     SGWFKGLGR"
     gene            515..1399
                     /locus_tag="VE170_16540"
     CDS             515..1399
                     /locus_tag="VE170_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ17102.1"
                     /translation="MRVVVTGGSGRLGQYVVRELFTHAHQVSSLDCVKPRECPTPTYA
                     VDLGKIDGLIDHFSSADAVIHLARIRFPYTETGFDTATQKWRFGDPVGDAERFHRNLA
                     IANNVLTAAEIAGIKKIVCGSSLAVYGLYYPSTEQQPDYLPVDETHPLRPQDPYGLTK
                     LLGEQLCDALSKRSGMAVTSLRFSGIYTEAHRAMLLDRNKNPLARGTGALWSYIDARD
                     AARACRVALEADLPGHQVFNIAAPNSLVDIPTRELARRYLPQVRDLRLGLDGTCSGYS
                     VTKAKNVLGFEAKLSLVN"
     gene            1458..2834
                     /locus_tag="VE170_16545"
     CDS             1458..2834
                     /locus_tag="VE170_16545"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ17103.1"
                     /translation="MMDLREFLKVLEEEHELQKIKAEVDPKHELGAICKIHNERPNNP
                     ALLFENVKGHNMSVVGQLLASDNRVALALGLSQANVFDETARRASQPIPTRLVASGPC
                     QEVVYEGQDVDVTKLPLCTNNPRDGGPYITAGHVIIRDPEYGNNLAIYRMMLVSKNEV
                     TIRFTPGHDGHDFIKNAEKRGQEKFQVAVCIGVPPAVYVASQFEPRAGVFELEIAGGL
                     AGEAVDVVKCRTIDLEIPALAEIILEGELTIPAKTGDEGPFGEFCGYTTAQVPNERVM
                     TIKAITHRRHPIYHNIWLGKPPHEHLYVDALTYAVAAYQELKPAYPALKKAYAPPWGV
                     SIVLILQMEKRLMRRGIVNNILASSLYTRSGKWKHVIVVDEDINMEDPNQVLWALTTR
                     FQPATDMFVIPRSITSSLEPSAEADGLTSKLMLDLTIKENFRGEVAEPTADMRQMVLQ
                     KWQEYGFK"
     gene            2990..>3351
                     /locus_tag="VE170_16550"
     CDS             2990..>3351
                     /locus_tag="VE170_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ17104.1"
                     /translation="MKPTKSKLTVADIPRIGPGTPAGEWFRRYWLVVSTAHELHDIPQ
                     AVKVLGEELVLFRDQNGQVGLLGEHCPHRGTSLEYGDIEDGGLRCPYHGWLFDVHGRC
                     LEMPAERKDSKFCEKVKQL"
BASE COUNT          755 a          921 c         1007 g          668 t
ORIGIN      
        1 attcaaaccg gcggcatgcc ggagggcctg gcggctctga ccgcaggcgc gctggacgcg
       61 gtggtattgt cgccgccgag taactttcgc gccgtccagg caggcatgca tgagctggaa
      121 gatgtcggtc agttgaaaat agttttcccg aacacgccgc tgtcggcgac gcgcacctac
      181 ctgcgcgcca accgcgacac cggcctgcgc ttcatgcgcg gcttcgccca gggattgcag
      241 cggctgcgca cggacaagga gttcagcatg aaggtcttgt ccaagtacac caaggtgacc
      301 gacagcgaaa ccctgggcca gctgcatcaa acctacggtg tgcgctacag cggcgaccgg
      361 attccctacg tgcggcccga gagtatcgag gagattctcc ggcgcacgcc cggcaaggaa
      421 gcgcgcgacg ccaaagccgc ggacttcatc gataacagct tgctgcaaga attggaaaag
      481 tcgggatggt tcaaaggttt ggggcggtga cctaatgcgc gtggtggtca caggggggtc
      541 ggggcggttg gggcagtatg tcgttcggga gctgtttacc cacgcccatc aagtgtcgtc
      601 gctcgattgc gtcaagccca gggaatgccc aacgccgact tatgccgtcg atctaggcaa
      661 gatcgatggg ttgatcgacc acttcagtag cgctgacgcg gtgattcacc tggcgcgcat
      721 tcgctttccc tacacggaga ccggcttcga taccgcgacg caaaaatggc gctttggtga
      781 tcctgttggg gatgccgagc ggttccatcg gaatctggcc atcgccaata atgtcctgac
      841 ggcagctgaa atcgctggta taaagaaaat cgtctgcggc tcgagcctgg cggtttatgg
      901 actttactat ccttccactg agcagcagcc ggattatttg ccggttgacg aaacccatcc
      961 gctccggccg caagatccct atggactgac caaactgctg ggagagcagc tctgcgatgc
     1021 cttaagcaaa cgaagcggca tggcggtgac aagcctgcgt ttttccggaa tctataccga
     1081 agcgcaccgg gcgatgctgc tcgaccgcaa taagaacccg ctggcgcgcg gcaccggcgc
     1141 gctgtggagt tacatcgacg cgcgcgatgc cgcgcgcgcc tgtcgggtgg cgctggaagc
     1201 cgatttgccc ggccatcagg ttttcaatat tgccgcgccg aatagcctcg tcgatatccc
     1261 tacccgtgag ctggcccgcc gttacctgcc gcaagtgcgc gatttacgcc tgggcttgga
     1321 cggcacttgc agtgggtata gtgtaactaa ggcaaaaaat gtcttagggt tcgaggcgaa
     1381 attgtctctg gtgaattgat tgtcattgcg gtgttcgcgg ccatgacggc gagtgcaaga
     1441 ggagcgaagt agtcatcatg atggacctac gggaatttct caaagtcttg gaagaagagc
     1501 atgaactgca aaagatcaaa gccgaagtcg atcccaagca tgaacttggg gcgatttgca
     1561 aaattcataa tgaaagaccc aacaatccgg cgctgctttt tgaaaacgtc aaaggccaca
     1621 acatgtcggt ggtcggccag ctattggcca gcgataaccg tgtcgcactg gcgttaggat
     1681 tatcccaagc aaacgtcttt gacgaaaccg cgcgccgggc aagtcagcca attccgaccc
     1741 ggttggttgc cagcggccca tgccaggaag ttgtctacga gggccaagac gtcgatgtga
     1801 ccaagttgcc gctgtgcacc aacaatccgc gcgacggcgg gccgtacatc accgccggcc
     1861 atgtgatcat tcgcgatccg gagtatggca acaatttggc gatttaccgc atgatgttgg
     1921 tgtcaaaaaa cgaggtgacg atccgcttta ctcccggcca tgatggccac gatttcatca
     1981 aaaacgccga gaagcgcggg caagaaaaat tccaggtcgc cgtatgcatc ggtgtgccgc
     2041 cggcggtcta cgttgcctca caattcgaac cccgtgccgg cgtctttgag ttggaaatcg
     2101 ccggcggcct cgccggtgaa gctgtggatg tggtgaaatg ccggaccatc gacctggaga
     2161 taccggcgtt ggcggagata atcctcgaag gggaactgac catcccggcg aaaaccggcg
     2221 acgaagggcc gtttggcgag ttttgcggtt acaccaccgc gcaagtgccg aacgagagag
     2281 ttatgacgat caaggcgatc acgcatcggc gccaccccat ctatcacaac atctggctcg
     2341 gcaagccgcc gcatgaacat ctgtacgtcg acgctctaac ctatgccgtg gcagcttacc
     2401 aggaattaaa gccggcctac ccggcgttga aaaaagccta tgcgccgccc tggggagtct
     2461 cgatcgtttt gattttgcaa atggaaaaac gtctaatgcg ccgggggatc gtcaacaata
     2521 tcctggcgtc atcgctctat acccgaagcg gcaaatggaa gcatgtgatc gtcgtcgacg
     2581 aagatatcaa tatggaagat cccaaccagg tgctctgggc gctgacgacg cgattccagc
     2641 cggcgacgga tatgttcgtg attccgcgga gcatcacgag ctctctcgaa ccctcggccg
     2701 aggcggacgg ccttacctcg aaattgatgc tcgacttgac catcaaggaa aattttcgcg
     2761 gcgaagtggc tgagccgaca gcggacatgc gccaaatggt tttgcagaag tggcaagagt
     2821 atggattcaa ataggcgcgc gacgcgcgct tcgcgcgccg gttcaaaagt tcaagggttc
     2881 aaaggttcaa ggttcggagc aagacttcgg agcatggacc gaatgatccg attgaacttt
     2941 gaacccttga accttgaaca agcaagaaag tctgaccgtg aataaaggta tgaagcccac
     3001 gaaaagtaaa ttgaccgttg ccgacatccc ccgcatcggc cccggcacgc cggccgggga
     3061 atggtttcgc cgttattggc tggtggtgag cacggcccat gagcttcatg acattcccca
     3121 ggccgtgaaa gttctcggcg aggagttggt gttgtttcgc gatcagaacg gccaagtcgg
     3181 tctgctcggc gagcattgtc cgcaccgggg gacgtccctc gagtatggcg atatcgaaga
     3241 tggcggtctg cgttgtccct atcatggctg gctgttcgac gtgcacggcc gctgcctgga
     3301 aatgccggcg gaacgcaagg acagcaaatt ctgcgagaag gttaagcagc t
//
LOCUS       DASQKS010000638         6438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5814730, whole genome shotgun sequence.
ACCESSION   DASQKS010000638 DASQKS010000000
VERSION     DASQKS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5814730"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..1472
                     /locus_tag="VE170_16555"
     CDS             471..1472
                     /locus_tag="VE170_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17105.1"
                     /translation="MSRTVRFFAIALYLLSMPGPSLSQSKELTRIRVSYSSIGAASLS
                     TWVAVDAGIFNKHGLDVGLIYIGGGPRAMSTTIANETQITQGAGTGSILAKIGGADTV
                     MFATLLNTTPQSLMVHPDLRSPQELKGKKLGVTRFGALSDFGVRKYLQKINLEPEKDV
                     AILQLGGLPEILAAMQTGAIHGGALSSPALTKAKHLGFKELMDLGNLGIQYPGLSYMT
                     TEAYIKAQRPVVVNFLKAIIESTHYLKRNKDLTLRVLKKYTKMDEPAVLDETYELFTQ
                     RYFRLVPTPSGEEVKTVLDQIKDKDPKARTTDYDSFIRTDLLREIEQSGFIKAMAKN"
     gene            1650..2648
                     /locus_tag="VE170_16560"
     CDS             1650..2648
                     /locus_tag="VE170_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ17106.1"
                     /translation="MATKLKFGLLLPHFCEYGSTELCVEGSKKAEAYGFDSVWVRDHL
                     VFEPHGMEGEDNTHIEGLLILAAVATVCKKLTLGTGTMISHRHPIHLAQSMAGLSTMC
                     DSNIIMGLGLGTFPHEFEAAGHTKTTLQDRANIAKVNAQLCRRFWAGEKISYKDDYYD
                     FKDVELKPTPSKNIPIWYGGGTPASARRAADYCDGWMPGRIPTATFNKMVKYLEEQCK
                     LAGRPMVTTGAIPITSIDKNRDVALSKVNTKGLINEGNAPSKKTWVKPKSGTFSTVED
                     IEGLLMAGTPADIARDTKRYEEAGLNHIVYDLRFRYADWYQQIDYLGQEVLPAVRA"
     gene            2758..3678
                     /locus_tag="VE170_16565"
     CDS             2758..3678
                     /locus_tag="VE170_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ17107.1"
                     /translation="MRTEKVNFYSEGIKLAGVLYLPDGEQGKPYPGIVQGPGFLGLKD
                     AKHYILMFEKLCAAGYACLCFDYRGWGDSEGDDRGWVMPKWQAEDIRSALSFMESRAD
                     IDPDRLATYGSGGTGGGNAVYVAAIDARVKCCVSYLGVSSGREWLHSMRREYEWIDYL
                     KSIEADRKQRALTGKSAIVSAREGGIMVQTPERVTTNIKKDVADKIPDGMPLACAEAI
                     LEYSPIDLVHKISPRGVLFIAVEGDAVTPEQQSFDLYQAAGEPKKLVLYKKTTHYGIY
                     NDYFDDVAANVVDWYNRHLKYHKVEVTESN"
     gene            3688..4710
                     /locus_tag="VE170_16570"
     CDS             3688..4710
                     /locus_tag="VE170_16570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYJ17108.1"
                     /translation="MNKISFGVRVPNSGPLSSIENIVKATKAAESMGFDAVWVHDHVV
                     WSSEMHRHHISSGAAEALTDTQDANFFEATTILSYLAAETKKIILGVACLVMPCRNPI
                     YAAKQYSTLDILAQGRLLVGVGLGSKATRESDEFGVFGVPYERRGDRTDEYIEAMKAI
                     WTQPLASYKGDFIQFNNAEVFPKPFQKPHPPVWVGGWMKLAAKRAGKYGEGWIPGWLS
                     PKEMKVGCDILNKTAEDNGRDPKKITIAVEKLATIAKTRDEGLNLAMPTLKTSSESYE
                     RDIDSMQFALDRHIFGSVDDVRRRVDEFIENGVQHFELKIIYPTMDSLTEQMELWAEN
                     ILPRYN"
     gene            4803..5300
                     /locus_tag="VE170_16575"
     CDS             4803..5300
                     /locus_tag="VE170_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011841077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ17109.1"
                     /translation="MNLQFNLNGKPWSVECEPADTLAEVMRNQLNLNGTKVSCEVQVC
                     GACTVLVDNLPVSACTYLAYEARGKNLLTVEGLEKADGTLHPIQQAFIDEFAFQCGFC
                     TPGMIMATKALLDEIPNPTREEIIHHMDGNICRCTGYVPIVAAIQRAAGMMSKEIPSE
                     RRKAN"
     gene            5278..>6438
                     /locus_tag="VE170_16580"
     CDS             5278..>6438
                     /locus_tag="VE170_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ17110.1"
                     /translation="MSAEKPTEFHIVNHSIPRRDGRVKVTGKAQYVADLRLIGMAYAK
                     VLRSPVAHAKIIAIDKSKAESHPGVYCVVTGYDLDGLNPYFGHAVKDHPLLAIDKVRY
                     TGEPVAAVVAVDERTAFEALEFIDVKYEELKGVFTPQEALAKDAPLLHDRKFEAGALR
                     GFEGEVTAGKGTNICQTHKIQWGDVEKAFKEAAAVIEGDYYFPMTYAYAMEPYVAIAD
                     VSDQGVNVYSSAQHPFMVRHDLKSIFDLPVSKVRLIVPFVGGGYGSKSYTKVEPLVAA
                     CSWKAKRPVKLQLTVEEAMLTTRSDDAYTWMRTAVDKNGKIIARQAKIMMNTGAYAEN
                     SPLASSKAAIRVLGPYLYEAVDITSYAVYTNTCPASSYRGFGVNQVALAAEVQ"
BASE COUNT         1536 a         1861 c         1763 g         1278 t
ORIGIN      
        1 acatagggat gcacctcaaa ttccttctgc ggcccggcca taaattacct ccatgaatct
       61 gcgccgaact tacttcccac agtgaagcag tgtcaacgct tggaatcata tgaatgagat
      121 cgcaccagaa gattgcgaag gccacgaagg ttttggacca attatattcc gaacttcgcg
      181 ctcttcgtgt cctccttcga aaatacatgt gaggggataa tatgcacatc gaagtgtctc
      241 cggggctccg tcatacccgc ggaagcgggt atccaggcta attcggcgga gaacgaacct
      301 ggattccgcg tgcgcggaaa tgacgaatcg aaaagatgag tatgtggggg ggaatccctc
      361 cgttttcatg ttctgtggga gtgcgtaaga tcatgattca cttcgtggtg aatacgtctt
      421 tgcttgtcag cggacaagga tgatatacga cggcctgtgg aggatttgcc atgagccgaa
      481 cggtacgatt ttttgccatc gcgctttatc tcctgtcgat gccgggcccc agcctcagtc
      541 agagcaaaga gctcacgcgg attcgtgtca gctattcctc gatcggcgcc gcgtcgcttt
      601 cgacctgggt ggcggtggat gccggcattt tcaataaaca tggccttgac gtcggtttga
      661 tttacatcgg cggcggtccg cgcgccatgt ccaccaccat cgccaacgaa acccagatca
      721 cccaaggcgc cggtaccggc agcatcttgg ccaagatcgg cggcgccgat accgtcatgt
      781 tcgccaccct gctcaatacc acgccgcaga gtttgatggt ccatcccgac ctgcgctcgc
      841 cgcaggaatt gaaaggcaag aagctcggcg tcacccggtt cggcgcgctc agtgatttcg
      901 gcgtgcgtaa atatttgcag aagattaatc tcgagccgga aaaagatgtc gccattctgc
      961 agcttggcgg cctgccggaa atattggcgg ccatgcagac tggcgccatc cacggcggcg
     1021 cgctgtcctc accggcattg accaaggcca aacacctggg attcaaagaa ctgatggatt
     1081 tgggcaatct cggtatccaa tatcctgggt tgtcgtacat gaccaccgaa gcgtacatca
     1141 aagcccagcg ccccgtcgtc gtcaatttcc tcaaagcgat catcgagtcg acgcattatt
     1201 tgaagcgcaa taaagatctc accctacgtg tgctcaagaa atataccaag atggacgagc
     1261 ccgccgtgct cgacgagacc tacgaattgt tcacccagcg ttatttccga ctggtgccga
     1321 cgccctcagg tgaagaagta aaaaccgtcc tcgatcagat caaagacaaa gaccccaagg
     1381 cacgcaccac cgactacgat agctttatcc gcaccgacct gctgcgcgag atcgagcaaa
     1441 gcggcttcat caaggcgatg gcaaaaaatt aaccggcgag gccgcagctg aagggtcgat
     1501 agtagctaac cacgctcagc aattttccta gatcacgcct cgatcggggc ggttttcact
     1561 gcggctaccc tcatggactt tgctaagaaa aatgtgataa gaattttgct tcaatcctga
     1621 attacttaaa atcataagga gagagaagca tggcgacaaa gctgaaattt ggattgctgc
     1681 tgccgcattt ttgcgaatac ggatctaccg aactatgtgt cgaaggatca aaaaaagccg
     1741 aagcgtatgg ttttgactcg gtttgggtca gggatcactt ggtgttcgag ccccatggca
     1801 tggaaggtga agataacacc cacatcgaag gcctgttgat cctcgcagcg gtggccacgg
     1861 tctgtaagaa gctgaccctc ggcaccggca ccatgatctc ccatcgccat ccgatccatt
     1921 tggcccaatc catggctggc ctcagtacca tgtgcgacag caatatcatc atgggtctcg
     1981 gtcttggtac cttcccccat gaattcgaag ccgccggcca taccaagaca accttgcagg
     2041 atcgcgccaa tatcgccaag gtcaatgccc aactttgccg ccgcttctgg gccggtgaaa
     2101 aaatcagtta caaggacgat tattacgatt tcaaggacgt cgagctcaaa cccacgccga
     2161 gcaagaacat tccgatttgg tatggcggcg gcacgccggc ttcggcgcgc cgcgccgcgg
     2221 attactgcga cggctggatg cccggacgaa tacccacggc gacgtttaat aaaatggtca
     2281 agtatctcga agagcagtgc aaactcgccg gccgaccgat ggtcaccacc ggcgcgattc
     2341 cgatcaccag tatcgacaag aatagagatg ttgcgctcag caaggtcaac accaagggtc
     2401 tgatcaacga aggcaacgcg ccgagcaaaa aaacctgggt gaagccgaaa tccggaacct
     2461 tttcgactgt cgaagatatc gaaggcctgc tcatggccgg caccccggcg gacatcgcgc
     2521 gcgacaccaa gcgctacgaa gaggccggcc tcaatcacat cgtttacgac ctgcggttcc
     2581 gctacgccga ttggtaccag cagatcgact atctgggcca agaagttctg cccgcggttc
     2641 gcgcgtaatt aaagttcaaa ccgttcaaac agttcaaacc gtccgatccg aatccgcaaa
     2701 acggtttgaa cgacttgaac ggtttgaact ttttgaacgg atttttggag atttaaaatg
     2761 cgtaccgaaa aagtaaattt ctacagcgaa ggcatcaagc tcgccggcgt gctctatttg
     2821 ccggatggcg aacaaggcaa gccttatccc ggcatcgtcc aaggccccgg ctttctcggt
     2881 ctcaaagatg ccaagcacta tatcttgatg ttcgagaagc tctgcgccgc cggttacgcc
     2941 tgtctgtgct tcgattaccg cggctggggc gatagcgaag gcgacgaccg cggctgggtc
     3001 atgcccaaat ggcaggcgga agacattcgc agcgcgctca gcttcatgga aagccgcgcg
     3061 gatatcgacc ccgaccggct cgccacctac ggctcgggcg gcaccggcgg cggcaatgcc
     3121 gtctatgtcg cggccatcga tgcgcgggtg aaatgctgcg tcagctacct gggcgtcagc
     3181 agcggccgcg agtggctcca ctcgatgcgc cgcgagtacg aatggatcga ttatttgaag
     3241 agcatcgaag ccgaccgcaa gcaacgcgcg ttaaccggca aaagcgccat cgtcagcgcc
     3301 cgcgaaggcg gcatcatggt gcagaccccg gagcgggtga ccaccaacat caagaaagac
     3361 gtggccgaca agatccccga cggcatgccg ctcgcttgcg cagaggcgat cctggaatac
     3421 agtcccatcg atttggtgca caagatttcg ccgcgcgggg tgctcttcat tgccgtagaa
     3481 ggcgatgcgg tgacgccgga acagcagtcc ttcgatttat atcaagcggc gggcgagccg
     3541 aagaagttgg tgctctacaa gaagaccacc cactacggca tttacaacga ttatttcgac
     3601 gatgtcgccg ccaatgtggt cgactggtat aaccgccacc tgaagtacca caaggtcgaa
     3661 gtcaccgagt ccaactagga gtagaaaatg aacaaaattt cctttggcgt gcgcgtgccc
     3721 aactcgggac cgctgtcgag catcgagaac atcgttaaag cgaccaaggc agccgaaagc
     3781 atgggtttcg acgcggtctg ggtgcatgac cacgtggtat ggagctcgga aatgcaccgc
     3841 catcatattt cctccggtgc cgccgaagcg ctgaccgaca cccaggatgc aaattttttt
     3901 gaagcgacga cgattctgtc gtacctggcg gcggaaacca aaaaaatcat cctcggcgtg
     3961 gcctgcttgg tcatgccctg tcgcaatccg atttacgccg ccaagcagta ttcgaccctg
     4021 gacattcttg ctcagggccg tttgctggtc ggcgtcggtt tagggtccaa agcgacccgc
     4081 gagtcggacg agttcggcgt ctttggcgtg ccctacgagc gccgcggcga tcgcacggac
     4141 gaatatatcg aagcgatgaa agcgatctgg acccaaccgc tggcttctta caagggcgat
     4201 ttcatccaat tcaacaatgc cgaagtattt cccaagccct ttcaaaagcc ccatccgcca
     4261 gtctgggtcg gcggttggat gaaactcgcc gccaagcgcg ccggcaagta cggcgagggc
     4321 tggattcccg gctggttgtc gccgaaagaa atgaaagtcg gctgcgatat tctcaacaag
     4381 accgccgaag acaacggccg cgatccgaaa aagatcacca tcgccgtgga aaagctggcc
     4441 accatcgcca agactcgcga tgaaggtttg aacctggcga tgccgactct caaaaccagc
     4501 agcgagagct acgagcgcga catcgacagc atgcagttcg ccctggaccg tcatatcttc
     4561 ggttcggtgg acgatgtgcg ccgccgtgtc gacgagttta tcgaaaacgg cgtgcagcat
     4621 tttgaactca agatcatcta tccgaccatg gatagcctga ccgagcagat ggagctgtgg
     4681 gccgagaata ttctgccgcg ctataactaa aaaaatggag taatggagtg ttggagtagt
     4741 ggatgattgg gttttcttag ccccagtact ccactactcc atcactccaa ttggacttac
     4801 ccatgaatct gcaatttaac ctaaacggca aaccctggtc ggtggagtgc gagccggcgg
     4861 atacgctggc ggaagtgatg cgcaatcaac tcaacttgaa tggcacgaaa gtgtcctgcg
     4921 aagtgcaagt gtgcggcgcc tgcaccgtgc tggtcgacaa tctgccggtg agcgcctgca
     4981 cctatctcgc ctacgaagcg cgcggcaaaa acctccttac cgtcgaaggt ttggaaaaag
     5041 ccgatggcac gctccatccg atccagcagg ccttcattga cgagttcgcc tttcagtgcg
     5101 gcttctgcac gccgggcatg atcatggcga ctaaagcgtt gttggatgag attcccaatc
     5161 cgactcgcga agagatcatc caccacatgg acggcaatat ctgccgctgc accggctacg
     5221 tgccgatcgt cgccgccatc caacgcgccg ccggcatgat gtccaaggag atcccaagtg
     5281 agcgccgaaa agccaactga atttcacatc gtcaatcatt ccattccgcg ccgcgatggc
     5341 cgggtcaagg tcaccggcaa agcgcaatat gtcgccgatc tgcggctcat cggcatggcc
     5401 tacgccaaag tgctgcgcag cccggtcgcc cacgcaaaaa ttattgccat cgacaagtcg
     5461 aaagccgaat cccaccctgg cgtctactgc gtcgtcaccg gctacgatct cgatggcttg
     5521 aatccctatt tcggccacgc cgtcaaagat catcccttat tggcaatcga caaagtgcgc
     5581 tacaccggcg agccggtggc cgcggtggtc gccgtcgacg agcgcaccgc cttcgaagcg
     5641 ttggaattca tcgacgtcaa atacgaagag ctcaagggcg tcttcacgcc ccaagaagcg
     5701 ctggccaaag atgcgccgtt gttgcacgac cggaaattcg aagccggcgc gctgcgcggt
     5761 ttcgagggcg aagttaccgc cggcaaaggc acaaatattt gccagacgca caagattcag
     5821 tggggcgatg tcgagaaggc attcaaggaa gccgccgcag tgatcgaagg cgactattat
     5881 ttccccatga cctacgccta cgccatggaa ccttacgtcg ccatcgccga tgtcagcgac
     5941 cagggcgtaa atgtttattc gtcggcgcag cacccgttca tggtgcgcca cgatctgaaa
     6001 tcgatcttcg atttaccggt aagcaaggtc cgtctgatcg tgcccttcgt cggcggcggc
     6061 tacggcagta agtcctacac caaggtcgaa ccgctggtcg ccgcctgctc gtggaaagcc
     6121 aaacggccgg tgaaactgca actcaccgtc gaagaagcga tgttgaccac ccgcagcgac
     6181 gacgcctaca cctggatgcg cactgcggtg gataagaacg gtaaaataat cgcgcgccag
     6241 gcgaaaatca tgatgaacac cggcgcctac gccgagaaca gcccgctcgc ctcgtccaag
     6301 gcggcgatcc gggtgctcgg tccgtacctg tacgaggcgg tcgacatcac cagctacgcc
     6361 gtctacacca acacgtgtcc ggccagctcg taccgcgggt tcggcgtcaa ccaggtggcg
     6421 ctcgcggcgg aggtccag
//
LOCUS       DASQKS010000639         6901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_786228, whole genome shotgun sequence.
ACCESSION   DASQKS010000639 DASQKS010000000
VERSION     DASQKS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786228"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1347
                     /locus_tag="VE170_16585"
     CDS             43..1347
                     /locus_tag="VE170_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17111.1"
                     /translation="MKLEKAGVATATIVSSEFWTLAQAESRARGMADLPLVEVPHPVG
                     TISLEALRSMAESVADAIAGKLITGNGASARGLTVPSHASAGSAAASLSVPSDPGAMF
                     SHFFDRGWTDGLPVLPPTVTAVQAMIAAGGKSAATVLGVIPPLNGVATVEKIAANAVM
                     AGCLPEYFPLVLAALRGMTQPGYNLDGLQTTTGNIAPLAIVNGPCRNALAINYGANVL
                     GQGWRANATIGRAIRFVLTNVGGARPGSFDKSTMGQPAKYTFCFGENEEESPWQPFHV
                     ERGLARNCDALHMFGASGIYSAVDMASQSATGLLKTFALTMTGGLASGVTSTEVLWVI
                     CPEHASILARDGYSKAKLREALFLHARVPYDKIADENLELLAKRRPLWFKPGGLREVG
                     VVDRPDDIWLVVAGGAGAKSAYIPGRTATRMQTVAVRERGAE"
     gene            1347..2363
                     /locus_tag="VE170_16590"
     CDS             1347..2363
                     /locus_tag="VE170_16590"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17112.1"
                     /translation="MAYWSDGAMDQNRQLATRLIYLWALLLPALVNAQSKSIIIGEGV
                     RGAMYLPAYIAEEKGFFKRRELDTRLVTFSRSNDINALVSGDIQFDLTSPDKVVHSAL
                     GGFPVKMVMGTARGLNLALMVHPSIRSAADLKGKSVAITGFSGLPYTGLLLCLRELGL
                     TKEQIVPLNIGGKTARFEALLGNRVPAAILDPPYTTLAAKEGYRLLVDLAPLDVPYLR
                     NIVAVSEKTLRDDSRTVARFVAGLAEGLQFYRDKSNKDDSMKILAKYLRLPLEKHRAM
                     IEEGYDTYREMMLKKPYADANAMKILMEIIAESNPKAKSVNLASLVDSSFVERLDREG
                     MFDR"
     gene            complement(2397..3113)
                     /locus_tag="VE170_16595"
     CDS             complement(2397..3113)
                     /locus_tag="VE170_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17113.1"
                     /translation="MCHRDPANRNRQRSEETRQTEVATLRNQLEQSTSALQERNFAFT
                     ILENGSREQVASMEQRLRAAERQLEKRDSEIAALNHQINELLNRPNIPGYTTEQLEQE
                     IAKKTSALNQEFTALQNQLSQRDELVKSKTNMFRKAEQDFRATIIELEQRLKDAEGMV
                     LRQKTELKDKEVVIQATAAKEVEIGKLIKRLSLECENLSNELQEKTRLMGQLDGKKPQ
                     TQPDKNVWRRMIGRLQDEPL"
     gene            3918..5000
                     /locus_tag="VE170_16600"
     CDS             3918..5000
                     /locus_tag="VE170_16600"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HYJ17114.1"
                     /translation="MAANKLKGLVLSGGRGTRLRPLTHTAAKQLVPVANRPILFHVLD
                     NLQKAAVNDVGIVVSPETGQAIRDAVGDGSAWGMTVSYIEQSEPLGLAHAVKVARPYL
                     GDDAFVMYLGDNLIGSGIERFREEFEKSAAVAAILLKEVDNPSSFGIAEVDAQQRVLS
                     LVEKPLQPKSNLALVGIYFFTQGIHGAIDKIAPSWRGELEITDAIQLLLSQGGKVLSH
                     RIDGWWLDTGKKDDLLTANTVVLDEWVQRKIDGEIDGLSQVTGRVQLGKGSRVVNSTV
                     RGPVVIGDDVLVENSFIGPFTSIGNGCRIVSSVLEHCLLLESARVQFVDRLEDSLLGK
                     NSAVVREQTSRHAYRLMIGDDSEVLL"
     gene            5000..5563
                     /gene="rfbC"
                     /locus_tag="VE170_16605"
     CDS             5000..5563
                     /gene="rfbC"
                     /locus_tag="VE170_16605"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HYJ17115.1"
                     /translation="MPFTFTPTNIADVIVIEPRVFPDERGFFMETYKYSEFAAHGIHE
                     TFVQGNQSHSERGILRGLHFQKQPKAQGKLVRAIAGTIFDVAVDLRRGAPSYGAWVGM
                     ELSAENKNMLYAPAGFAHGFCVVSAAADILYMTTAEYAPELESGIAWDDADLDIAWPV
                     RAPQLSPRDRQWPRLRDADTGFVFHAG"
     gene            5585..6625
                     /gene="rfbB"
                     /locus_tag="VE170_16610"
     CDS             5585..6625
                     /gene="rfbB"
                     /locus_tag="VE170_16610"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HYJ17116.1"
                     /translation="MMKTIFVTGGCGFIGSNFVRLLSTFTDYRIVNFDKLTYAGNLEN
                     VADISRERMRFVQGDICDREMVERTLDEERPWAVVNFAAESHVDRSILDSTPFLQTNI
                     GGVQALLEAVRRVAVERFLHISTDEVYGDKEGKAISNEDSLLAPSSPYAASKAAADLL
                     CFSYRRTYGLPILITRSSNNYGPYQFPEKLIPLLLRNGLGGLDFPVYGDGLQIRDWIH
                     VEDNCRALLAVLEQGQIGSIYNIGTSEERSNYAVVEAICSALAAHKGVDLGQLTARIR
                     YVTDRPGHDRRYALDTAKVRKELAWTPRVDFPSGLQQTVNWYLSHADWVARVTSGEYR
                     KYYESVYTRNWS"
     gene            6634..>6901
                     /locus_tag="VE170_16615"
     CDS             6634..>6901
                     /locus_tag="VE170_16615"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HYJ17117.1"
                     /translation="MDRVVVIGSSGQLGHDLVEVLRVADRFEVITLDHSQIECTAVAS
                     VRQALLPLKARTFINCVAFDRVDDCESLARQAFEVNAIAALNVAR"
BASE COUNT         1355 a         1856 c         2109 g         1581 t
ORIGIN      
        1 gccgattgag gctcgtgcac gtcgtggagt gtccacgact cggtgaaact ggaaaaggcg
       61 ggggtcgcca ccgcgacgat agtgagcagc gagttctgga cgctggctca agcggagtcg
      121 cgggcgcgcg gcatggctga cctgccgctg gttgaagtgc cccatccggt cgggacaatc
      181 tcgctggaag ctctacgctc aatggccgaa tcggtggccg atgcgattgc cggtaaattg
      241 atcaccggca acggtgccag tgcgcgcggt ctcacagtgc cgtcgcatgc cagcgcaggc
      301 agcgcggctg ccagcttatc cgtgccgagc gatcccggcg cgatgttcag tcactttttc
      361 gatcgcggtt ggaccgacgg tttgccggtg ttaccgccga cagtaacggc ggtgcaggcg
      421 atgattgccg ccggcggcaa gtcggcggct acggtactag gtgtgattcc gccgttgaac
      481 ggggttgcca cggttgaaaa gatcgcggcc aatgcggtga tggcgggctg tttgccggag
      541 tattttccgc tcgtgctagc ggcactgcgc ggcatgaccc agccgggcta taacctcgat
      601 ggattgcaaa ccaccacggg caatatcgcg cccctggcga tcgtcaatgg accttgccgc
      661 aatgctttgg cgatcaacta cggcgccaac gtgctcggcc agggctggcg cgccaacgcg
      721 acgatcggcc gggcaattcg attcgtcttg accaatgtcg gcggcgctcg gccgggcagt
      781 ttcgacaaat cgaccatggg gcagccggcc aaatatactt tttgtttcgg tgaaaacgaa
      841 gaagaaagtc catggcagcc gtttcatgtc gaacgcggct tggcgcgcaa ttgcgatgcg
      901 ctgcacatgt tcggcgctag cgggatctat agcgcggttg acatggcgag ccagtcggca
      961 accggtctgc tcaagacttt cgcgctgacg atgaccggag gtttggccag tggcgtgacc
     1021 tcgaccgaag tgctttgggt gatttgccct gaacatgcgt cgatcctggc gcgagatggt
     1081 tattccaaag cgaagctgcg cgaagcgttg ttcctccacg cgcgcgtgcc ctatgataag
     1141 atcgccgatg aaaacttaga gctgctcgcc aaaaggcggc cgctgtggtt taagcctggc
     1201 gggttgcgcg aggttggcgt cgtcgaccgg cccgatgata tttggctcgt ggtcgccggc
     1261 ggcgcgggcg ccaagtcggc atatattccg gggcgaaccg cgacgcgaat gcagaccgtg
     1321 gcggtgcgcg agcgaggagc ggagtagtgg cgtactggag cgatggagca atggatcaga
     1381 atcggcagct cgcgacgcgg ctgatttatt tgtgggcgct gcttttgccg gcgctggtaa
     1441 atgcgcaaag caagtcgatc atcattggcg agggagtgcg cggggcgatg tatttgccag
     1501 cctacatcgc cgaggagaaa ggcttcttca agcgccgtga actcgatacc cgcctggtga
     1561 ccttcagtcg cagcaacgat atcaacgcct tggtgtctgg cgatattcaa ttcgatctga
     1621 cgtcgcctga caaagtcgtg catagcgcgt tgggcggctt tccggtgaag atggtgatgg
     1681 gcacggctcg tgggttgaat ctcgccttga tggtgcatcc gtcgattcgt tcggcagccg
     1741 atctcaaagg caagtcggtg gcgatcaccg gcttttccgg cttgccgtat accggcctcc
     1801 tgctctgttt gcgtgagctc ggtctgacca aggagcagat cgtgccgctc aatatcggcg
     1861 gcaagaccgc ccgcttcgaa gccttgctcg gcaatcgcgt gccggcggcg attcttgatc
     1921 cgccctacac aaccctggcc gcgaaggaag ggtaccgatt gctggtagat ctcgcgccgc
     1981 tcgacgtgcc gtatttgcgc aacatcgtcg cggtctcgga gaaaactttg cgcgacgatt
     2041 cccggacggt ggcgcgcttc gttgccgggt tggccgaggg tctgcaattt tatcgtgaca
     2101 agagcaacaa agatgacagc atgaagatct tggcgaaata tctgcgcctg ccgctggaga
     2161 aacaccgcgc catgatcgag gaaggctacg acacctatcg tgagatgatg ttgaaaaagc
     2221 cctatgccga tgccaatgcg atgaaaatac tgatggaaat aatcgccgag tccaacccca
     2281 aggccaagag cgttaactta gcgtctcttg tcgactcgtc atttgtcgag cgtttagacc
     2341 gagaagggat gttcgaccgt taatgggtcg tcccgcggcg cgctgcggga gaagctctac
     2401 agcggctcgt cctgaagccg gccgatcatg cggcgccaga catttttatc gggttgggtt
     2461 tgcggctttt tgccgtctag ttggcccatc agccgggtct tttcctgcaa ctcattgctc
     2521 aggttttcgc attcgagaga gagccgcttg atcagcttgc caatttcgac ttcctttgcc
     2581 gcggtcgctt gaatgaccac ctctttgtct ttgagttcgg ttttttggcg cagcaccatg
     2641 ccctcggcgt ctttgaggcg ctgttccagc tcgatgatag ttgcgcgaaa atcctgttcg
     2701 gctttgcgaa acatgttggt ttttgactta accagttcat cgcgctggct gagttggttt
     2761 tgcagcgctg tgaattcctg gttgagggct gaggtttttt tggctatttc ctgttcgagc
     2821 tgctcggtcg tgtagccggg gatgttgggg cggttgagca gctcgttgat ctggtggttc
     2881 aatgccgcga tctcggaatc gcgtttttcg agttggcgct ccgcggcgcg caggcgctgc
     2941 tccattgacg ccacctgctc gcgtgagccg ttttcgagta tggtgaaagc gaaattgcgt
     3001 tcctgaagcg ccgaagtact ttgctccagc tgattgcgta acgtcgcgac ttcggtttgg
     3061 cgggtttctt cgctgcgttg gcggtttcgg tttgccggat cgcggtgaca caagcgcgat
     3121 ccgcgaggtt gcttaacttg ccgatatagt cgagcagctg gttggcctga gtctcgatct
     3181 cggccaaacc ggaggcatcg tcgaaagata aattgggtac gtcgctagtg ctcttcgccg
     3241 gctcggcgtc cggttggctg cgaactatcg atacgacatt ttcattggcg cgcattgtaa
     3301 aattcctccg ccgatttcca cgggacagtg ctgctacaat ttgagcaatt gtcgtaccat
     3361 ctgggggtcg tgatgcaatg attataagta atcggaaacg tgagcttatt tttagtatta
     3421 tggcatcaca gaggtggtta atagcgaaac ttggccaata acgtccaagg cttgtaaaca
     3481 agagcaattt catcgaccga aattatccca tgcggttgcg gatatttccc gatgcgccca
     3541 ttaaccacag cagtggggtg aaaagcagtc gattttttaa attcgctcgg gtgctaaatc
     3601 cgagcgtgaa tcgctcggag cggctcggcg cgttgcggcg cgcggcaaag tttgcatggt
     3661 tggatgagta tattttgccg agccgctcgg cgcagaacga tggtgattcc aatcgacaaa
     3721 tccgttgagc catcgataaa attctcttgc acgggttcgg tgacatcctt gttgaataaa
     3781 tcgcaggcgt atggcgcgag gaaagtcaca gcagctgaaa ttcatggtca ttattgatgg
     3841 aaaaaaagat ctgttgaagg taatctgcgc tacatgagat ttgcaaggtg cttcttcacc
     3901 aagctttgag tcgatccatg gccgcgaata agctaaaagg gctggtcctg agcggcggcc
     3961 gcggtacccg gttgcggccg ctaactcaca ccgcggcgaa gcagctcgtg cccgtggcta
     4021 atcggccgat cttgtttcat gttctcgata acttacaaaa agccgcagtc aatgatgtcg
     4081 gcattgtcgt ttcgcccgag accggccagg cgattcgcga cgcggtgggt gacggcagtg
     4141 cctggggtat gaccgtttct tatatcgagc agagcgagcc ccttggcctc gcccacgcgg
     4201 tgaaagtggc acggccctac ctgggcgacg atgcgtttgt catgtatctg ggcgacaatc
     4261 tcattgggtc gggtatcgaa cggtttcgcg aagaattcga aaaatccgcc gcggtcgcgg
     4321 cgattttgct caaggaagtc gacaatccgt cgagcttcgg catcgctgaa gtcgacgctc
     4381 aacagcgcgt gctcagtctg gtggaaaagc cgctgcaacc gaaatccaat ctagcgctgg
     4441 tcggcattta ttttttcacc cagggtatac acggcgccat cgataaaatc gcgccgtctt
     4501 ggcgtggcga actcgagatc accgacgcga ttcaactgct actcagccaa ggcggcaagg
     4561 tgttgagcca tcgtatcgac ggctggtggc ttgacaccgg caagaaagac gatttgttga
     4621 cggctaatac cgtcgtgctc gacgagtggg tgcaacgaaa gatagatggc gagatcgacg
     4681 gcctcagcca ggtgaccgga cgagttcagc tgggtaaggg cagccgcgtg gtcaatagca
     4741 ccgtgcgcgg tccggtggtg atcggcgacg atgtgctggt ggagaactct ttcatcgggc
     4801 cgtttacttc catcggcaat ggctgccgca tcgtttcgtc cgtgctcgaa cattgcctgc
     4861 tgttggaaag cgcgcgagtg cagtttgtcg accgtctcga agatagcctg cttggcaaaa
     4921 actccgccgt ggtgcgcgag caaaccagcc gccacgccta ccgtttgatg atcggcgacg
     4981 actccgaagt attgctctga tgccgtttac ttttacaccc accaacattg ccgacgtaat
     5041 tgtcatcgag ccgcgggtgt ttcccgacga gcgcggtttt ttcatggaga cttacaaata
     5101 ttcagaattc gccgcccatg gtattcacga aacgttcgtg cagggcaatc agtcgcactc
     5161 ggagcggggc attttacgcg gcttgcactt tcaaaagcag ccaaaggccc aaggcaaact
     5221 tgtgcgcgcc atcgccggca cgatcttcga tgtcgcggtg gatctgcgca ggggagcacc
     5281 gagctatggc gcatgggtgg gcatggagtt gagcgcagaa aataaaaaca tgctctatgc
     5341 gcccgcaggg ttcgcccatg gcttctgtgt tgtcagcgcc gcggcggata tcttgtatat
     5401 gaccacggcg gaatacgccc cggagctgga gtccggcatt gcttgggatg acgccgatct
     5461 cgacatcgcc tggccggttc gggcgccgca actttcgccg cgcgatcgcc agtggccgcg
     5521 gttgcgcgac gccgacacgg gctttgtctt tcacgctggc tgatcgccgc gggtttcggc
     5581 agaaatgatg aaaactatct ttgtcacagg cggttgcggt tttatcggca gtaacttcgt
     5641 tcggttactg tcgaccttta cggattaccg cattgtcaac ttcgataagc tgacctacgc
     5701 cggcaatttg gaaaatgtcg ccgatattag ccgggaacgc atgcgctttg tccagggcga
     5761 tatctgcgat cgtgaaatgg tcgagcgcac gcttgacgag gagcggccct gggcggtggt
     5821 caacttcgcc gccgagtccc atgtcgatcg cagcatcctc gactcgacgc cgtttctcca
     5881 gaccaatatc ggcggcgtac aggcgctgct cgaggccgta cggcgcgtgg cggtggagcg
     5941 ctttctgcac atctccaccg acgaagtcta cggcgacaag gaaggcaagg cgatctccaa
     6001 tgaagactcg cttttggcgc cgagcagtcc ctacgcggcg agcaaagccg ccgccgatct
     6061 gctttgtttc tcctatcgcc gcacttatgg attgccaata ctgatcacgc gcagctcgaa
     6121 caattatgga ccttatcagt ttcccgagaa gctcattccg ctgcttttgc gcaacggtct
     6181 cggcggtctc gactttccgg tttacggcga tggcttgcag attcgcgatt ggattcatgt
     6241 cgaggacaac tgccgggcgt tgttggcggt attggagcaa ggccagatcg gctcgattta
     6301 taatatcggc accagcgaag agcgcagtaa ttatgccgtc gtcgaagcaa tctgcagcgc
     6361 actcgcagcg cacaagggcg tcgatttggg acaactcacc gcgcgcatcc gctacgttac
     6421 cgaccgcccc ggccacgacc ggcgctacgc cctcgatacc gccaaagtgc ggaaggagct
     6481 ggcctggacg ccgcgagtcg atttcccaag cggtttacag cagaccgtaa attggtattt
     6541 gtcccacgct gactgggtag cgcgggtgac ttccggtgaa taccgcaaat actatgaatc
     6601 ggtttacacc cggaattgga gctagatctc ctgatggatc gggtcgttgt tatcggcagt
     6661 agcgggcagc tgggccatga tctggtcgag gtgctgcggg ttgccgatcg tttcgaggtg
     6721 ataaccctcg atcatagtca gatagaatgc acggcagtgg cctcagtccg ccaggcgttg
     6781 ttgccgctca aggctcgaac cttcatcaac tgcgttgctt ttgaccgtgt cgacgattgt
     6841 gaatcgctgg cgcgccaagc cttcgaggtt aatgccatcg ccgccctcaa cgtcgcgcgg
     6901 g
//
LOCUS       DASQKS010000640         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1617448, whole genome shotgun sequence.
ACCESSION   DASQKS010000640 DASQKS010000000
VERSION     DASQKS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617448"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VE170_16620"
     CDS             <1..1494
                     /locus_tag="VE170_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HYJ17118.1"
                     /translation="VASVHGQSAVAARDQSPLPGQSWFADFANAREKFAIGSWLGGAI
                     GVGLWGYLWFFTPDIGLSPSATKQLAILLFTLVYWVFRVLPDYGVALIFALGFILTGL
                     ANPEVVLGGFASTTWFMTLGVLGLGAAITSTGLFYRFSLQLVRYFPLKYNWQIVALGF
                     MGIIVMALIPQQSARTAIISQMLLNLSESLGYKNPSRASTGLFVASFLGLGQLGFLFL
                     TGSTTSLIAWGLLAPEVREQFTWGYWFLAALPPTLIVAAIIVGATMLLYQPESQSQIS
                     YKMVQTQLEILGPLSKYEWITLVVLCATVTGWLTGSMHKIDGAWVALLALCLLINTGV
                     LGLGMLKKSIDWEMLIYMGATLSIPTLLTEAKIDQWLVGLFAPLVVPFSATPALAFVV
                     IALITYAVRLVFTSFLTVVTLVIALLPLAVELQINPWVMIMIVLIASEMWFFSFQLDW
                     HSLAYATTDGKGFSYQEMKRVRWFYAAAYLTALVAAIPYWRYLGLMR"
     gene            1591..2568
                     /locus_tag="VE170_16625"
     CDS             1591..2568
                     /locus_tag="VE170_16625"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HYJ17119.1"
                     /translation="MDTPTQTLDAAIHSTYRIHFHGRGGSLFGIQIVNLFLSLISLGI
                     YSFWGRVKVRRYLASQSEFAGDRFAYHGTGQELMIGWTKAMVLFGMPLFILAVLQVIV
                     GIESDLFDALQILIYIVVGIFIPIAMVNARRYRLTRLSWRGVRFSFRGGVRDFMGIYI
                     KGWLLTVVSLGFYYPFWQNRRQTFMVGNSFFGNRQFAFDGRGRDLFLTFVLHMILTLP
                     TFFLCWLWYWARVQRYYAEHTSFQAARLRSTVSGGSVFALWFGNGILFILTLGLGWSW
                     ITVRNMRYYASHMTMVGTLDFAGVMQEVQTATATGEGLSGFLDLDFDLG"
     gene            2590..3711
                     /locus_tag="VE170_16630"
     CDS             2590..3711
                     /locus_tag="VE170_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ17120.1"
                     /translation="MQLQWFGYYFDGQSAIRRNARIYLEPGNLRIDLDDGTMLTWPYA
                     AIRQTQGAYAGEHVRLEHGGEPVEVLLIDDNSFLTSLHQLMPSRAGGFHNPAKRFQRF
                     QLTIAAAGASIALVIGSYFWGIPMLATIAAPWVPVSWEEQLGQSMLEYVAPAEQRCSD
                     PGRLAKLENLVGAMVVTQGETKYRFKIHVVNQPMFNALAAPGGHLVIFRGLLDRTENA
                     EELAGVMAHELQHILKLHVTRSLLEYASTSFLVAAVLGDTSGIVSFGVEAAQMLAQMR
                     FSRQHELEADEAGLQMLLAARIDPAGMIAFFEAVGKDEGQMAPALSYISTHPQTELRI
                     ARLKSLIAASAVSYQKLLPDDSWEDIKKICPAPVIPAKQ"
     gene            3783..4289
                     /locus_tag="VE170_16635"
     CDS             3783..4289
                     /locus_tag="VE170_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HYJ17121.1"
                     /translation="MAVHEHKEKAQKNVRYFVITVSDSRDENTDTSGQTIKQLLSGAG
                     HHAAGYRIVKDEPLEIAALLEEALGKADVEAVIVNGGTGIAPRDGTYEVVSRFLDKKL
                     DGFGEIFRYLSYLDIGSAAIMSRAAAGTARGKVLISLPGSKGAVTLAMEKLILPEICH
                     MVSQLQGR"
     gene            4298..4531
                     /gene="moaD"
                     /locus_tag="VE170_16640"
     CDS             4298..4531
                     /gene="moaD"
                     /locus_tag="VE170_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018868167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HYJ17122.1"
                     /translation="MKFFAILRERAGTAETTQELSDGATVGDLWHGLQKTYPKLDVPG
                     IRLLYAVNQNYVHLDEQLRDGDEVVFLPPVSGG"
     gene            4538..4969
                     /locus_tag="VE170_16645"
     CDS             4538..4969
                     /locus_tag="VE170_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HYJ17123.1"
                     /translation="MFRVQNVAIDLNEPVRYVTDAEAGAIATFIGTTRNNNEGRRVIA
                     LDYEAYPEMAEKELKRIGAEATQKWPICRMAIIHRLGPVQIGEASVVIAVSSAHRDAA
                     FAASRFAIEEIKKTVPIWKKEVFDGGEVWIGTQTGQRFDPS"
     gene            5065..5775
                     /locus_tag="VE170_16650"
     CDS             5065..5775
                     /locus_tag="VE170_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreH"
                     /protein_id="HYJ17124.1"
                     /translation="MDNSLFAAFGLGLLLGIQHALDPDHLIAVSMIVSEHKNFKWASL
                     IGAFWGLGHTTTLFLVGLLVIGLRLTISESVAAGLEFLVAIMLVVLGLNVLRRTFGPA
                     QVHLHTHTHNPETHAHFHVHEKPQQDHGHNHPFKAMRRPFFVGMVHGLAGSAALMLLV
                     LGTIPSPLAGLAYIVIFGFGSVGGMLLLSGIISLPFVLTAQRFSAVNRWIRLAAGFTS
                     VAFGLLLGWKIGSDQHWF"
     gene            complement(5798..6448)
                     /locus_tag="VE170_16655"
     CDS             complement(5798..6448)
                     /locus_tag="VE170_16655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYJ17125.1"
                     /translation="MTEIMTWIMDSIRAHGAWSVFAGVIIESVIVPIPSPLIIMGAGF
                     ILIGADLKFFEALPPILLQIVLPGAIASTLGAYIGYAIGYYGGKPMVDRWERLLGFSW
                     KDVESLEKRFRHGQVNTTIFFLRALPIFPLSVISAAAGLLRLPLNQFSLWTFYGTIPR
                     CLLLGYLGWGLGETYQGIAKGIDKAEGIVSAIIILAIMAIIVWLRARVRGKILDNG"
BASE COUNT         1310 a         1842 c         1941 g         1551 t
ORIGIN      
        1 gttgcaagcg ttcacggcca atcggcagtt gctgcccgcg atcagtcgcc cttgcctggg
       61 cagagctggt ttgccgattt tgccaacgca cgggagaagt tcgcgatcgg ttcttggttg
      121 ggtggcgcaa tcggtgtcgg cctgtggggc tacctgtggt tttttacccc cgatatcgga
      181 ctgtcgccga gcgcgaccaa gcagttggcc attttgctct tcaccttggt ctattgggtg
      241 tttcgtgtct tacctgacta tggagtcgcg ctgatttttg ctttgggatt tattctcacc
      301 ggtctggcca atcctgaagt agtcctaggc ggtttcgcca gcaccacctg gttcatgact
      361 ctgggcgtgc tcggtctcgg cgccgcgatc accagcacgg gcctgttcta ccgtttttca
      421 ctccagctag tgcgctactt tccactcaaa tataactggc aaattgttgc cctgggtttc
      481 atgggtatca tagtcatggc gctgatcccg cagcagagcg cgcgcaccgc gatcatcagc
      541 cagatgctgc tcaacctctc ggagagcttg ggttacaaaa atccgtcgcg cgcctctacc
      601 ggcctattcg tcgccagttt cttgggtctc ggtcagttgg gttttttatt cttgacgggt
      661 tcgacgacca gtttgatcgc ctggggatta ctggcgccgg aagtccgcga gcaattcact
      721 tggggctatt ggtttttggc ggctttgccg ccgacgttga tcgttgccgc gatcatcgtc
      781 ggtgccacca tgctgttgta tcaaccggag tcgcagtcgc agatttccta caaaatggtg
      841 cagacccaac tggagattct cgggccgctc tcgaaatatg aatggattac cctggtcgtt
      901 ctgtgcgcca ccgtgaccgg ctggctgact gggtcgatgc acaaaatcga cggcgcctgg
      961 gtggcgctgc tcgccctttg cctgttgatc aacaccggcg tgctcggctt gggcatgctg
     1021 aaaaaaagca tcgattggga aatgctcatt tatatgggcg cgacgctaag cattcccacg
     1081 ctactcacgg aggcgaaaat cgatcagtgg ctggtcggcc tgttcgcacc gctggtggtg
     1141 ccctttagcg ccacgccggc gctggcgttt gtcgtcattg ccctgatcac ctacgcggtc
     1201 aggctggtgt ttaccagttt cctgaccgtg gtgactttgg tgatcgcact tttgcccttg
     1261 gccgtcgaac tgcagatcaa tccctgggtc atgatcatga tcgtattgat cgcatcggag
     1321 atgtggtttt tctcgttcca gctcgactgg cattcgctgg cctatgcgac caccgacggc
     1381 aagggcttca gctaccagga gatgaagcgc gttcgctggt tctatgccgc agcctatttg
     1441 accgcgctgg tggcggcgat tccctattgg cgttacttgg gactgatgcg ctgagccatc
     1501 ctgagaatgg ctcggcggcg cctttgccga tggtgtatca tctttcttgg aaggggcgcg
     1561 gcgcaagaca gctcggtaat ggcgacagac ttggacactc cgactcagac attggatgcg
     1621 gcgatacatt cgacctaccg gattcatttt catggccgcg gtgggtcact attcggcatc
     1681 cagatcgtca atcttttctt gagcttgatt tcactcggta tttattcttt ttggggccgg
     1741 gtcaaagtgc gccgctacct ggcgagccag agcgaatttg ccggcgatcg cttcgcctat
     1801 cacggcaccg gccaggagtt gatgattggc tggaccaagg cgatggtgct tttcggtatg
     1861 ccgctgttca tcttggcggt cttacaggtc atcgtcggca tagagtccga cctgttcgat
     1921 gccttgcaga tacttattta catcgtcgtc ggtattttca ttcccatcgc catggtcaat
     1981 gcccggcgtt accgcttgac ccgtctctct tggcgcggtg tgcgattttc gtttcgcggc
     2041 ggcgtgcgcg acttcatggg catctacatc aaaggttggc tgctgaccgt ggtgagtttg
     2101 gggttttact atccgttctg gcagaaccgt cggcagacct ttatggtcgg caattccttc
     2161 ttcggcaatc ggcaattcgc tttcgacggc cgtggccgcg atctcttttt gacgtttgtt
     2221 ttacatatga tcctgaccct gccgacattt tttctctgct ggctttggta ctgggcgcgg
     2281 gtgcagcgct actatgccga gcatacgtct ttccaagcgg cgcgcttgcg gtcgacggtc
     2341 agcggcggca gcgtgttcgc tttgtggttc ggcaacggga ttctcttcat tctgaccttg
     2401 gggctcggct ggtcatggat caccgtgcgc aacatgcgct actatgccag ccacatgacg
     2461 atggttggga ctctcgattt cgcaggtgtc atgcaagaag ttcagacggc aaccgcgacc
     2521 ggtgagggac tttccggttt tctcgatctc gacttcgatc tcggctgagc aggttcgcgc
     2581 gccgcaacga tgcaactgca atggtttggc tattacttcg acgggcaaag cgccatccgg
     2641 cgcaacgcca ggatttatct cgagcctggc aatctgcgca tcgatcttga cgacggcacc
     2701 atgcttactt ggccctacgc ggcgatccgg caaacccagg gcgcctacgc cggcgaacat
     2761 gtccggctgg agcatggcgg cgaaccggtt gaagtcttgt tgattgatga caacagcttc
     2821 ctcacgtctt tgcatcagct catgcccagc cgcgccggtg gtttccataa cccggccaag
     2881 cgtttccaac gattccaact caccattgct gcagctggcg cgtcgatcgc gctggttatc
     2941 ggaagttatt tttggggcat cccgatgctc gcgacaatcg ccgcgccgtg ggtgccggtg
     3001 tcgtgggaag agcaactcgg tcaaagcatg ctggagtatg tcgcaccggc ggagcagcgc
     3061 tgcagcgatc cgggacggct ggcaaaactc gaaaacctgg tcggcgccat ggttgtgacc
     3121 cagggcgaga ctaagtaccg cttcaagatt cacgtggtca accagccaat gttcaatgcc
     3181 ttggccgccc caggcggcca tctcgtcatc tttcgcggtt tgttggatcg caccgagaat
     3241 gccgaagagc tcgccggtgt catggcccat gagctgcaac atatcctcaa gctccatgtc
     3301 acacgctcgc tgctcgaata cgcatcgaca agttttttgg tcgccgcggt gttgggcgac
     3361 accagcggca tcgtttcctt cggcgtcgag gcggcgcaga tgttggcgca gatgcgtttt
     3421 agccgccagc atgagctgga agccgacgag gccggtttgc agatgctctt agcagcgcgc
     3481 atcgatcccg caggcatgat cgcgttcttc gaagcggtag gtaaagacga gggtcaaatg
     3541 gcgccggcgt tgtcctatat ttcgacccac ccgcagaccg agttgcgcat cgcccgactc
     3601 aagagtttga tcgcggcgag cgccgtgagt taccagaaac tgctgccgga tgattcgtgg
     3661 gaggacatta aaaagatctg tccagccccg gtaattccag cgaaacagta gaaaattcgc
     3721 tggtttgaat tcaccagggt cgattgctat agtcgccaat gaaatttccc ggagaggcgc
     3781 ccatggcggt ccacgagcac aaagaaaaag cccaaaaaaa cgtgcgctat tttgtcatca
     3841 cggtaagcga tagccgcgat gaaaacaccg acaccagcgg tcagacgatt aaacagttgc
     3901 tcagcggcgc aggccatcac gccgccggct accggatcgt caaagacgag ccgctagaga
     3961 tcgctgcatt actcgaagaa gcgctgggta aagccgatgt cgaagcggta atcgtcaacg
     4021 gcggcaccgg gatcgcgccg cgcgacggca cctacgaggt cgtcagccgc tttctcgaca
     4081 aaaagcttga cggtttcggc gagatttttc gctatctgag ctacctcgac atcggttcgg
     4141 cggcgatcat gagccgggct gccgccggca ctgcgcgcgg caaagtcttg atttcgctgc
     4201 cgggttcgaa aggcgcggtg accttggcga tggaaaagct gattctgccg gaaatttgcc
     4261 acatggtttc gcagctccaa ggaagatgaa ggttcgcgtg aagttcttcg cgatcctgcg
     4321 tgagcgcgcc ggaaccgccg aaacgaccca ggaactcagc gacggcgcca cggtcggcga
     4381 tctctggcat gggttgcaaa aaacctatcc taagctcgat gtgccgggga ttcggctgct
     4441 ctacgccgtc aaccagaatt acgtccacct agacgagcaa ctccgggacg gcgacgaagt
     4501 ggtatttctc ccgccggtga gtggaggctg agatcttatg tttcgggtgc agaacgttgc
     4561 tatcgaccta aacgagccgg tgcgttatgt cacggatgcc gaggcgggcg ccatcgcgac
     4621 gtttatcggc accacgcgca acaacaacga agggcgccga gtaatcgcgc tggactacga
     4681 agcctatccc gagatggcgg agaaagaact gaagcgaatc ggcgccgaag caacgcaaaa
     4741 atggccgatc tgccgcatgg cgatcattca ccgattgggc ccggtgcaga tcggcgaggc
     4801 gagcgtggtc atcgccgtgt cgtcggcgca ccgggatgcg gcctttgccg ccagccgctt
     4861 cgccatcgaa gaaatcaaaa agaccgtgcc gatttggaag aaagaagttt tcgacggcgg
     4921 cgaggtatgg atcggcacgc agaccggcca gcgattcgac ccctcataga ctaaacggcg
     4981 acgcgcattt tagatctact ttggcgattt tcgatcgcgt ccgaaagcca aatctaaaag
     5041 cgaccgttca gatcaatttg gacaatggac aattcgttat tcgccgcctt cggtttggga
     5101 ctgctcctgg gcatccagca tgcactcgat cccgatcatt tgatcgcggt gtcaatgatc
     5161 gtcagcgagc ataagaactt caaatgggcg tcgttgatcg gcgccttttg gggcttgggc
     5221 cacaccacga cgttgtttct ggtcggcctg ctggtgatcg gtctgcgcct gacgatttcc
     5281 gagtcggttg ccgcaggctt agagtttctc gtagcgatca tgctggtggt gctcggtctg
     5341 aacgtcttgc gtcgcacttt tggcccggcg caagttcatt tgcacactca tacccataat
     5401 cctgaaactc atgctcattt tcacgtccat gaaaagccgc agcaggacca tggccacaac
     5461 catccgttca aagcgatgcg ccggccgttt ttcgttggca tggtgcatgg cctcgccggc
     5521 agcgcggcgc tgatgttgct cgtgctcggc acgatcccgt caccattggc cggccttgct
     5581 tatattgtga ttttcggttt cggttcggtt ggcgggatgt tgctgttgag cggcatcatc
     5641 agcttgccgt ttgttctcac ggcgcaacgc ttttcggcgg tgaaccgttg gatacgcctg
     5701 gcggcgggat tcaccagtgt cgcgtttggc ctattgctcg gctggaaaat cggttcggat
     5761 caacactggt tttagagcaa ttttcccgag tcgcaggtca cccattatcg agaatcttgc
     5821 cgcgcacgcg ggcgcgcagc cagacgatga tcgccataat cgccaggatg ataatcgccg
     5881 aaacaatccc ttccgctttg tcgatgcctt tggcgattcc ttgataggtt tcgcccaggc
     5941 cccaaccgag gtagccaagc agcaggcagc ggggaatcgt gccgtagaaa gtccacaggc
     6001 taaactggtt gaggggcaga cgcagtagtc ccgccgccgc ggaaattacc gaaagcggga
     6061 aaatcggcag agcgcgaaga aaaaaaatcg tcgtgttcac ctggccgtgg cgaaagcgtt
     6121 tttccaagga ttcgacgtct ttccaactaa aacccaacaa cctctcccag cggtcgacca
     6181 tcggcttgcc gccgtagtag ccgatggcgt agccgatgta ggcgcccagt gtcgaggcaa
     6241 tggcgccggg caggacgatc tgcagcagga tcggcggcag ggcctcgaaa aatttgagat
     6301 cggcgccgat caagataaag cccgcgccca tgatgatcag cggcgacggg atcggcacga
     6361 ttaccgattc aataataacg ccggcaaata cgctccaagc gccgtgggca cggatggaat
     6421 ccatgatcca agtcatgatt tctgtcaagg cgaatttctc cccggtaaat tttgactatg
     6481 gtagcagcct ggcagcgcta ataccattac acaggagttt ggcggcggcg cagagagccc
     6541 atgacatcag atgagtttga gcacggtgag cagatagagc atggcgaggg cgaagtaggc
     6601 ggagcaggca atcagcatga aatagaaatt catttttccc ggtt
//
LOCUS       DASQKS010000641         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1220745, whole genome shotgun sequence.
ACCESSION   DASQKS010000641 DASQKS010000000
VERSION     DASQKS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220745"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..681)
                     /locus_tag="VE170_16660"
     CDS             complement(283..681)
                     /locus_tag="VE170_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYJ17126.1"
                     /translation="MAERTVATRPQEKASSSREETRSQERYITPPVDIYETTNGLVVT
                     ADLPGVAKDGLDVRVENNLLTIRGKAAHAAPGGPVYREYGLVNFFRQFELNEKVDQSK
                     ISAELKHGVLTLNLPKAEEAKPRKIEVKVT"
     gene            complement(709..1167)
                     /locus_tag="VE170_16665"
     CDS             complement(709..1167)
                     /locus_tag="VE170_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYJ17127.1"
                     /translation="MAEWNSFADVEALRREIGRAFEDFGFEQGQPQRVAFLPGREPRR
                     YPLVNLLEDKDNIYIEALTPGVDPQSINLNALQNRLTVAGEKSGIPGDVKPEAFHRNE
                     RASGKFVRTIDLPVEIDETGIEAEYKDGLLVVTLPKAEKAKPKRISVKIA"
     gene            complement(1490..3112)
                     /gene="groL"
                     /locus_tag="VE170_16670"
     CDS             complement(1490..3112)
                     /gene="groL"
                     /locus_tag="VE170_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995052.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYJ17128.1"
                     /translation="MAAKDIRFSEDARNRVLRGVELLADAVTVTLGPKGRNVVLEKSW
                     GSPTVTKDGVSVAKEIQLEDKFENMGAQMVKEVASKTSDLAGDGTTTATVLSRAIFSE
                     GLKLVAAGHDPMSIKRGIDKAVAKVVEELKSISKPTRDRDEIAQVGTISANNDKAIGA
                     ILAEAMDKVGKEGVITVEEAKGLETTLDLVEGMRFDRGYLSPYMVTDPERMECVYDDC
                     YILINEKKISSMKDLLPVLEAVAKAGKPLVIIAEEVEGEALATLVVNKIRGTIKVVAV
                     KAPGFGDRRKAMLEDIAILTEGKMIAEELGIKLENVTVKDLGRAKRVIIDKDNTTVVE
                     GAGKKSAIEGRITQIRAQIEDTTSDYDREKLQERLAKLAGGVAVIKVGAATEIEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRASAVLANMRVSDDEKIGVRIIQKAVEEPL
                     RWIVTNAGAEGSVVLDKVRNGKGAFGFNAATEEYEDLVKAGIVDPTKVVRVALQNSAS
                     VAGLLLTTEAMIADKPEKKKDAPAMPHDHEDY"
     gene            complement(3127..3417)
                     /gene="groES"
                     /locus_tag="VE170_16675"
     CDS             complement(3127..3417)
                     /gene="groES"
                     /locus_tag="VE170_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HYJ17129.1"
                     /translation="MATKIRPLHDRVIVKRIAEEEKTKGGIIIPDTAKEKPQEGKVIA
                     VGPGKYEEGKLIPLGVKAGDKILFGKYSGSEIKLDDEEHLIMREDDILGVIE"
     gene            complement(3529..3918)
                     /locus_tag="VE170_16680"
     CDS             complement(3529..3918)
                     /locus_tag="VE170_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ17130.1"
                     /translation="MIKLLSIASALFCGVILSGVPSWAQNQAEGRKLYASYCSTCHGD
                     KGKGDGMAAKSLPAKPTDHTNGAIMNQMNDKFLLDIISKGGSAVGKSSFMPAWGNALD
                     EKQIRDVMAFIRTLAVPAYKGETPASK"
     gene            4070..>4342
                     /locus_tag="VE170_16685"
     CDS             4070..>4342
                     /locus_tag="VE170_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ17131.1"
                     /translation="MIKIWGRTDSSNVQKVLWCCGELGVPFERLDWGGKFGGNQDPQY
                     AAMNPNGLVPTIKDGDFVLWESNSIMRYLNAKYGNGKLLPASAEGMA"
BASE COUNT          904 a         1230 c         1083 g         1125 t
ORIGIN      
        1 aaacgaccga ccttggtttt gtggatatca atcgattaga agagcggagg gccgtatggt
       61 cgcagctcca tgcgcgacgg tattgcctcg taagcttgga aatgccagca tctgactgcc
      121 taactgcatg gccgcctgac cgcacgggaa gtcccgccgg attgatcccg cttcgaatca
      181 atcacatcga atcgatccat gtgagccgaa attcggccgg tcaattcaaa ggtaggcttc
      241 ccctctgatt gttaatagct tcgccctaca ggacagttcg tcttatgtta ctttaacttc
      301 gatctttctc ggtttggctt cctcagcctt cggcaaattc agggttaaga cgccgtgctt
      361 aagctcggcg gagatcttgc tctgatcgac cttctcgttc aactcgaatt ggcgaaagaa
      421 atttaccaat ccgtattccc gataaaccgg accgccgggc gccgcatgcg ccgctttgcc
      481 gcgaatagtc aataggttgt tttccacgcg cacgtccaga ccgtctttgg cgacgcccgg
      541 taaatcggcg gtcaccacca gtccattggt ggtctcgtat atatctacgg gcggcgtaat
      601 atatctttcc tgactgcgag tttcttctcg gcttgatgaa gccttttctt gcgggcgcgt
      661 cgctacagtt ctctcggcca tggctagttt cctccttgtc atgagttatc aggcgatttt
      721 gacgctgatt cgtttgggct tggctttctc ggcttttggt agggtcacca ccagcagtcc
      781 gtctttgtat tctgcctcta tgccggtttc atcgatctca acgggtaaat caatggtacg
      841 aacaaacttc ccactggctc gctcatttcg atgaaatgct tccggcttga cgtcccctgg
      901 aatgccgctc ttctctcccg ctacggtcag acgattttgc aacgcgttga gattgatgga
      961 ttgaggatca acgccgggag tcaacgcctc gatgtaaatg ttatccttat cttcgagtaa
     1021 atttaccaac ggatatcgcc gcggttcgcg gccggggaga aaagcgacgc gctgcggctg
     1081 accttgttca aagccaaaat cttcaaaagc tcggccgatt tctcgccgca acgcttccac
     1141 gtcagcaaag gaattccact ctgccatagt cctatacctc cttgatttat tgttttcccg
     1201 acaaactagt ttcgacggta agttagtcac gcgcgcaagc ttgtcaagat ggattgataa
     1261 ttccaattaa aaactctatc gggattctgt gatacgccgc agcgctgccg caaacttcct
     1321 cagcggaacg gaataagcct gggtaccaca aaacaattaa tacggagcct aatagccgat
     1381 gggtcgcggc cttggttaat ttgaaacttt aaccggggta ttaacccaac aaaaaaaagg
     1441 cgttcggttt cccgaacgcc ttttttgctt tccaaacgtc gattagctct taatagtctt
     1501 cgtgatcgtg tggcatggcc ggagcgtctt ttttcttctc cggtttgtcg gcgatcatgg
     1561 cttcggtggt aagcagcaga cccgccacag aagcggagtt ctgcagagcg acacgcacga
     1621 ccttggtcgg gtcgacgatg ccggctttga ctagatcctc atactcctcg gtagcggcgt
     1681 tgaaaccgaa cgcgcccttg ccgttgcgaa ccttatccaa aactaccgag ccctcggcac
     1741 cggcattggt gacgatccaa cggagcggct cttcgaccgc tttctgaatg attcggacgc
     1801 cgattttttc gtcgtccgaa actctcatgt tggcgagcac ggcagaagcg cgaatcagcg
     1861 ccacgccgcc gccgggaacg atgccttcct cgactgccgc acgcgtggcg tgcagcgcgt
     1921 cttcgacgcg ggcttttttc tctttcattt cgatttcagt ggcagcgccg actttgatga
     1981 cggccacacc accggccaac ttggctaaac gttcttggag tttttcgcgg tcgtagtccg
     2041 aagtggtatc ctcgatctgc gctcgaatct gcgtaatgcg gccttcgatt gccgacttct
     2101 taccggcgcc ttcgactaca gtggtgttgt ccttatcgat gatcacccgt ttggcccggc
     2161 caagatcctt cacggtcacg ttttcaagct tgatgcccaa ttcttcggcg atcatcttac
     2221 cttcggtcaa gatcgcgatg tcttcgagca ttgccttgcg ccggtcgcca aaacccgggg
     2281 ctttgactgc gaccactttg atcgtgccgc gaatcttgtt cacgaccaaa gtcgccaagg
     2341 cttcgccttc gacttcttca gcgataatca ccagcggctt gccggccttg gccaccgcct
     2401 ccaggaccgg tagtaagtcc ttcatgcttg agattttttt ctcgttgatc aaaatgtagc
     2461 aatcgtcgta aacgcactcc atgcgctcgg gatcggtaac catgtagggt gagagatagc
     2521 cgcggtcgaa gcgcatgcct tcaacgaggt caagggtggt ttccaaaccc ttggcttctt
     2581 caacggtgat tacaccttct ttgccgacct tatccatcgc ttcggcaaga attgcaccga
     2641 tcgctttatc gttatttgcc gaaatcgtgc cgacctgggc gatctcatcg cggtcacggg
     2701 tgggcttgga gatcgatttg agttcttcga ccactttggc aacggcttta tcgatgcccc
     2761 gtttgatgct catcgggtca tgcccggcgg cgaccagctt gagcccttcg ctgaaaatcg
     2821 cgcgcgacag cactgtcgct gtcgtcgtgc cgtcaccggc taggtcggaa gtcttgcttg
     2881 cgacttcttt gaccatttgg gcgcccatgt tttcaaattt atcttcgagc tgtatttctt
     2941 tggccaccga aacaccgtcc ttagtgacgg tcggcgaacc ccaagatttt tccaaaacga
     3001 cattgcggcc tttgggtccc aatgtgacgg ttaccgcatc agccagtaat tctacgccgc
     3061 gcagcactcg attgcgcgcg tcttcactaa aacgaatatc cttagcagcc atcttccctg
     3121 tcctccctat tcaatcacgc cgagaatgtc atcttcacgc atgatcagat gttcctcgtc
     3181 gtccaatttg atctcactgc cggagtattt gccgaatagg attttatccc cggccttgac
     3241 cccgagaggg atgagtttgc cctcctcata tttgcctgga cccaccgcga tgactttccc
     3301 ctcctgcggc ttttccttgg cggtatcagg gatgatgatt ccccctttgg ttttttcctc
     3361 ttccgcaatg cgtttcacga tcaccctatc atgtagggga cggatcttag ttgccatagc
     3421 accctcctca ataaaacgaa gatttagata tccagaacga ttggatgcgg gcaatgtaat
     3481 ctcgcaaagc caatcgtcaa gggggtcgga tgaaaaatta gcgatttttc atttactcgc
     3541 cggggtttcg cccttgtagg caggcacggc cagggtgcga atgaaagcca tcacatcgcg
     3601 gatctgcttt tcgtccaggg cattgcccca ggcgggcatg aagctcgatt taccgaccgc
     3661 gctgccgccc ttgctgatga tatccaggag aaacttatcg ttcatctgat tcatgattgc
     3721 gccattggtg tgatcggtgg gtttggccgg cagcgatttg gccgccatgc catcgccttt
     3781 gcctttgtca ccatggcaag tcgaacaata gctggcgtag agtttacgcc cctccgcttg
     3841 attctgcgcc cagctcggaa ccccgctcag gatcacgcca caaaagagcg cagacgcaat
     3901 cgacagtagc ttgatcatcc ccgattcgct cgctttccgt gcaaattgat ccgtatttcc
     3961 tagcagatat taaccggaaa caacagcgcc gcccgcagtt cctctggctc tacccaatag
     4021 tgaaaaaggg tgataagtag cagcgaaact gccacgagcg gaggcgataa tgattaaaat
     4081 atggggtcga accgactctt ccaatgtaca aaaagtactg tggtgctgcg gcgagctggg
     4141 cgtgccattc gagcgcctcg attggggcgg caagttcggc ggcaaccaag atccgcaata
     4201 tgctgcgatg aaccccaacg gcctggtacc gacgatcaag gacggcgact tcgtcctctg
     4261 ggaatccaac agtatcatgc gctatctcaa cgccaagtat ggcaacggca aattattgcc
     4321 tgcgtccgcc gaaggcatgg cc
//
LOCUS       DASQKS010000642         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4526080, whole genome shotgun sequence.
ACCESSION   DASQKS010000642 DASQKS010000000
VERSION     DASQKS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4526080"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VE170_16690"
     CDS             complement(<1..984)
                     /locus_tag="VE170_16690"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYJ17132.1"
                     /translation="MIAPKPISSLEVGVKAEEAKRRKREGIVIAVAALMVAALIFFEV
                     QSPDISLENSLGSNIVFFFIININIILLVLLVFLVVRNLVKLVFERRRRLLGSRLQSR
                     LVLAFVALSIVPSLLLFAIAGGLLTHSFDRWFDSKVESALQGSLEIGQTYYQNSANNA
                     MFFARRLSQTITENGLFESARLAELRRLIEDKQRDYNLGTVEIYSAESEAIVAVSDKS
                     KNAANLKPDAEFLARALRGIGVTWTEPLGEGDVIRGGAPIFGAEKRIVGVAVVDYYVP
                     KSLTKRAAQISQSYEQYKYLTFLKAPVKNSYILTLFLITLVIMFSATWWGFY"
     gene            complement(1024..1236)
                     /locus_tag="VE170_16695"
     CDS             complement(1024..1236)
                     /locus_tag="VE170_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HYJ17133.1"
                     /translation="MAGLAKTLIILGMVLVIAGVLVSFSDRLPWLGHLPGDIHIERER
                     FSFHFPLTTCIIISIVISLVAYLFKR"
     gene            complement(1482..2510)
                     /gene="ruvB"
                     /locus_tag="VE170_16700"
     CDS             complement(1482..2510)
                     /gene="ruvB"
                     /locus_tag="VE170_16700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYJ17134.1"
                     /translation="MLDSHRTDEDSRFELSLRPQGFDEYVGQDGVKDNLKVAIAAARG
                     RGDVLDHILFHGPPGLGKTSIAYIIAREMGVSIKATAGPIIERAGDLAALLTNLDHGD
                     VLFIDEIHRLNHVVEEILYPAMEDYEIDIMVGQGPAAKSIKLPLKKFTLIGATTRSGL
                     LTSPLRDRFGQSFRLDFYDAVSLTRILKRSAAILSVEQDQDGIAEIASRSRGTPRIAN
                     RLLRRVRDYAQVKSDGKITKAVTVDALRMLEVDRLGLDKMDTMLLLAMIDKFAGGPVG
                     VETLAAAIGEEKDTIEDVYEPFLIQAGFIQRTPRGRVATVLAYNHFGRQPGAAKPAVG
                     KGQPDLFE"
BASE COUNT          594 a          775 c          750 g          557 t
ORIGIN      
        1 ataaaatccc caccaggtgg ccgaaaacat gatcaccaaa gtgatcagaa atagcgtcaa
       61 gatataactg tttttaaccg gcgccttgag gaaggttaaa tatttgtatt gctcgtagga
      121 ctgcgaaatt tgcgccgccc gtttggtcag actcttgggg acataatagt cgaccacggc
      181 gacgccaacg atgcgctttt ccgcgccgaa gatcggcgcg ccgccacgga tcacgtcgcc
      241 ttcccctaaa ggctccgtcc aggtcacgcc gatgccacgc aacgcccgcg ccaagaactc
      301 ggcgtcgggt tttaaattcg ccgcgttttt cgacttgtcg ctaacggcga cgatcgcttc
      361 gctctccgcc gaatagattt ccaccgtgcc aaggttgtag tcgcgctgtt tatcctcgat
      421 caaccggcgc aactcggcca gccgggcgct ttcgaatagc ccgttctcgg tgatggtttg
      481 gctcaaccgg cgggcaaaaa acatcgcgtt gttagcggaa ttttggtaat aagtctggcc
      541 gatttccaaa gacccttgca gcgcgctttc caccttggaa tcgaaccagc gatcgaagga
      601 atgggtcaaa aggccgccgg cgatggcgaa caacaacagg ctcggcacga tcgatagcgc
      661 gacgaaagcc aagaccagac gggactgcag gcgcgagccg agcaagcggc ggcggcgttc
      721 gaaaaccagc ttgacgagat tgcgcacgac gagaaaaacc aacagtacca gcagaatgat
      781 gttgatattg atgatgaaaa aaaacacgat gttgctgccg agggaattct ccagcgagat
      841 gtccggcgat tgcacttcga aaaagatcaa cgccgccacc atcaacgccg cgaccgcgat
      901 gacgatgccc tcgcgcttgc gccgtttggc ctcttcggct ttgacgccga cttcgaggct
      961 agagattggc tttggagcta tcatgggcag tggcaaattc ccggcgagat tcctgcgaga
     1021 attctaacgt ttgaacaagt aagcgacaag cgaaatcacg atactgatga taatgcaggt
     1081 ggtcagcgga aaatggaaac tgaagcgttc ccgctcgata tggatatcgc ccggcaagtg
     1141 gccaagccat ggtaaccggt cggaaaatga caccagcacg ccggcgataa ccaggaccat
     1201 gccgagaatt atgagcgtct tagccaaacc agccacactc cgattaccaa atcgaaccgt
     1261 taatattttc tcggcgatgg gcggaaacct ctggttttca atgcagtcgg gttcggtgat
     1321 gcccgggccg cagctggtta tccgctaccg ctcaaaggca tgacggacag tgtcggtcac
     1381 ctagattccc gcttccgcgg gaatacgata aagcgaaagt cggcttgcag ccgacatctt
     1441 cgaaatacct cggatcattg ccgagcatcg gcttatcaac ctcattcaaa caaatccggc
     1501 tggcctttgc cgacggccgg cttggcggcg cccggttgcc ggccaaaatg gttgtaggcc
     1561 agcacggtag ccacccggcc acgtggcgtc cgttgaatga acccggcttg gatcaaaaac
     1621 ggctcgtaaa catcctcgat ggtgtccttc tcttcgccga tggcggcggc caaagtctcg
     1681 acgccgaccg gcccgccggc aaacttgtcg atcatggcca ggagcagcat ggtgtccatc
     1741 ttgtcgaggc ccaagcgatc gacctcgagc atcctgagcg catccactgt caccgctttg
     1801 gtgatcttgc cgtcgctttt cacctgggcg tagtcgcgca cccggcgcag caagcggttg
     1861 gcgatgcgcg gcgtgccgcg cgagcggctg gcgatctcgg cgataccgtc ttgatcttgt
     1921 tcgacgctga gaatcgccgc cgaacgcttg agaatgcgcg tcaacgatac cgcgtcgtaa
     1981 aaatccaggc gaaaactctg gccgaagcgg tcgcgcaatg gcgaggtcag caggcccgag
     2041 cgagtggttg cgccgatcaa agtgaatttt tttagcggca acttgatcga cttcgccgcc
     2101 gggccctgcc cgaccatgat gtcgatctcg taatcttcca tggccggata aagaatctct
     2161 tcgaccacat ggttgagccg atgaatctcg tcgatgaaca acacatcgcc atggtctagg
     2221 ttggtcaaga gcgccgccag atctccggcg cgctcgatga tcggccccgc ggtggccttg
     2281 atgctgaccc ccatctcgcg ggcgatgatg taggcgatag atgttttgcc cagtcccggc
     2341 gggccgtgga aaagaatatg atcgaggacg tcgccgcgcc cccgcgccgc cgcgatggcg
     2401 actttgagat tgtctttaac cccatcttgg ccgacgtatt cgtcgaagcc ctgcggccgc
     2461 aagctcagct cgaatctgga gtcttcgtcg gtgcgatggc tgtccaacat ggtcggtatg
     2521 cgttcttcct acacgcccgg ggatggattt aaaacccgtc tctgggtgcg aattcgaaat
     2581 cgattttttg ccttattagc ggccgctcta gccgttccaa atcgtccact cgctatctcc
     2641 ggttaatgac ccatctgctg cagggtctcg cgcagg
//
LOCUS       DASQKS010000643         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_787230, whole genome shotgun sequence.
ACCESSION   DASQKS010000643 DASQKS010000000
VERSION     DASQKS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787230"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VE170_16705"
     CDS             <1..514
                     /locus_tag="VE170_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17135.1"
                     /translation="LLQSTFPSGRLYVTQHGLPNILCFRERDRDHLAWRVPIDDDSHC
                     SFQLDVTHITEGDEGEAYKNRHKAREGKLGRSPREIGEAVLRGDLRIQDIEGPERSNI
                     VWIQDYATQVGQGPLDLRKKETLGRSDRALILIRKIWERELQNLEAGKPLKQWQRTER
                     LAALHSQAND"
     gene            709..1140
                     /locus_tag="VE170_16710"
     CDS             709..1140
                     /locus_tag="VE170_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYJ17136.1"
                     /translation="MKLTLDKANVIADRTLAKARDAKYRPMCVAVLDEGGHLKVLKRE
                     DAASILRPDIAIGKAWGAVGMGESSRSLSERLKERPAFLGALSVMSQGKVVPVAGGVL
                     VMDGADGNEIIGAVGVSGGTAEEDEACAIAGIEAAGFVAKS"
     gene            complement(1497..1982)
                     /locus_tag="VE170_16715"
     CDS             complement(1497..1982)
                     /locus_tag="VE170_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYJ17137.1"
                     /translation="MPILKRLKEKLDEAKIPYEVYTHALAYTAQEVAARQRVSGNELA
                     KVVMIEADEQLVMAVIRGNDKVNLHIVEDSLAARNVRLATEDEFIARFPECEIGAMPP
                     FGNLFGVKVYVDPALTKDEYIYFNAGNHVQTVRLKYTDFARLVQPQVVRLVGDRGKFA
                     A"
     gene            2341..>2737
                     /locus_tag="VE170_16720"
     CDS             2341..>2737
                     /locus_tag="VE170_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HYJ17138.1"
                     /translation="MKIPLQITFRDMAPSPAIEDNIREKAAKLDELHDEIMGCRVTVE
                     APHRHHHKGKAYVVRIDLTVPGGELVVNREPKHIVPKKAKAGDEPDNDFAESHEPSKH
                     AAHEDIYVAIRDAFNAAGRKLQDHARRRRG"
BASE COUNT          673 a          798 c          745 g          521 t
ORIGIN      
        1 gttgttgcaa tcgactttcc cgagcggccg gctctacgtc acgcagcatg gcttgccgaa
       61 catcctgtgc ttcagagaac gcgaccggga tcatctcgcc tggcgcgtgc cgatcgacga
      121 cgacagtcat tgcagcttcc aactcgatgt cacccatatt accgaaggcg acgagggcga
      181 agcttacaaa aatcgccaca aagcgcgcga aggaaaactc ggccgttcac ccagggaaat
      241 cggtgaagca gtgctgcgcg gcgacctgcg cattcaggac atcgaaggac cggagcggtc
      301 caacatcgtc tggatccaag actacgccac ccaggtcggc cagggccccc tcgatctgag
      361 aaaaaaagaa accctcgggc gctccgaccg cgcgctgatt ctgattagaa aaatctggga
      421 gcgcgaactg caaaacctcg aagccggcaa accgctcaag cagtggcagc gcaccgaacg
      481 gctggcggcg ctgcattcgc aggcgaacga ctaaacgcgt ggtcacgaaa tatacgaaaa
      541 atcgttgctc cgaatccgtc attcccgcgc aggcgggaag gaatccaggc gcaatatcct
      601 tcgctcacgc agcgattggt agattcccta tcaaccggcg ctggaggata tctttgcgcc
      661 aatgaacgac tagaattccg tcagcatgga agtcttggag gttagaaaat gaaactgact
      721 ctcgataaag ccaacgtcat cgccgaccgg actctcgcca aagcgcgcga tgcgaaatac
      781 cgtcccatgt gcgtcgccgt cctcgacgaa ggcggccact tgaaagtgct caagcgagaa
      841 gatgctgcca gcatcctgcg ccccgacatc gccatcggca aagcctgggg cgccgtcggc
      901 atgggcgaat cgagccgttc gctaagcgag cggctcaaag aacggccggc atttctcggc
      961 gccctatcag tgatgtccca aggcaaggtc gtaccggtag ccggcggcgt gctcgtcatg
     1021 gacggcgcag acggtaatga aataattggc gcggtgggtg tcagcggcgg cacagccgag
     1081 gaagatgaag cctgtgctat tgcaggcatc gaggccgcag gctttgtcgc caaatcctag
     1141 cgcggccacc ggaactcgga ctttctcccg cagaggcgca aaggccgcaa aagtcggaga
     1201 atgagggtga acaacattcg attggttttt ctccggcgaa cttggcgtct ttgcgcccct
     1261 tcgactttgc acaggacatg ctttgcgcga gacatttccc aatccgagtc tctgaaatct
     1321 ccggggcatt tgcaaaaacc ggcatcgaag gcgaagcggt caatcaacgc cacctcgcgc
     1381 gcacggggaa cttgggccgg gcgcccaatg aaatcggctg gcggattcgt tccgaacggc
     1441 gcacctctcc cgcagcaacg ctcggtgcca cgattgcgcc gacaagctta agcatcctac
     1501 gccgcgaact tgccgcgatc gccaaccaac cgcaccacct gcggctgcac caaccgtgcg
     1561 aagtctgtgt attttagtcg cacggtctgg acatgattgc cggcgttaaa gtaaatatac
     1621 tcgtccttgg tcaaagccgg atcgacatac actttcacgc caaacagatt accgaacggc
     1681 ggcatcgcgc cgatctcgca ttcggggaaa cgtgcgatga actcgtcttc agtcgccaac
     1741 ctgacgttcc gtgcggcgag gctatcctcg acgatgtgta ggttgacctt gtcgttgcct
     1801 cgaatcaccg ccatcaccag ctgctcatcg gcttcgatca tgacgacctt ggccagctca
     1861 ttgcctgaga cacgttgccg agcggcaacc tcctgggccg tgtacgccag cgcatgagtg
     1921 taaacctcat atgggatctt cgcttcatcg agtttctctt tgagtctttt tagaatgggc
     1981 atgaccggct cctttctcaa acacattttg tcgtcgatgt tgcagacgca attcaaagat
     2041 gcgcaagaat tatgcccgct agtgacaatt ttctcagcga atgactactg aaatcatata
     2101 gaatcgagct cactttgggt gctaggggcg tcgaggctta tcacgactga caagcacgca
     2161 gcgacaaatt cgcagcactg agaagggcgc gccaggaccg tcgacgggtc aagaaattct
     2221 ccaaataaat caattcattt ataggcgcta aaaaaaacca agtggcatat ctcttgccgt
     2281 tttttgattc agggttaaca atagattgcg cgtaggaaca acaaccggag gaaaggaaat
     2341 atgaaaattc ccttgcagat caccttccgt gacatggcac cgtcgccagc tatcgaagat
     2401 aatatccgcg agaaagcagc caagctcgac gagctgcatg acgagatcat gggctgccgg
     2461 gtgaccgtcg aagcgcccca tcgccatcac cacaaaggca aagcttacgt ggtgcggatc
     2521 gatttaaccg ttcccggcgg cgaactggtc gtcaatcggg aaccaaaaca tatagtgccg
     2581 aaaaaagcta aggccggcga cgaaccggat aatgacttcg ccgaatcaca tgaaccgagc
     2641 aaacatgcgg cccacgaaga catctatgtc gccatccgcg acgccttcaa cgccgctggc
     2701 cgaaagcttc aggatcacgc gcggcgccgg cgcggca
//
LOCUS       DASQKS010000644         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2025165, whole genome shotgun sequence.
ACCESSION   DASQKS010000644 DASQKS010000000
VERSION     DASQKS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025165"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VE170_16725"
     CDS             complement(<1..680)
                     /locus_tag="VE170_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYJ17139.1"
                     /translation="MTSLGVGLAVPDWPTTFGYNMFLYPWSKMIGGIFYEHSHRLVAS
                     CVGLLTIALTLTLWLKEPRRWLRWLGVAALALVIVQGVLGGLRVVLLQPTLAILHACF
                     AQAFFALTVSLALFTAREWRNPPQGAPLSDGGRLRRLAAITTALIYCQIVFGAMLRHT
                     GARLDAHLLFAGLVAVHVILLLLRISREHGAATALMRPGYLLGGLLVGQLLLGALSYL
                     AKFTALIP"
     gene            complement(825..2585)
                     /locus_tag="VE170_16730"
     CDS             complement(825..2585)
                     /locus_tag="VE170_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYJ17140.1"
                     /translation="MSESAVAHGAHGHHPKPSFISHYIFSLDHKMIGRQFLFVGLLML
                     LIGGLLAMLVRWQLAWPETPVPGLGFVSEPYIYPGPNGGGIIPPQTYNALFTMHATIM
                     IFFVVMPIMVGTFGNFLIPLMIGAGDMAFPKLNMLSFWFGAISGVIMLSSFFVPGGPA
                     AGGWTGYATLSAKAQYTGVEWGQHLWIIALMVNGISSLMGAINYITTVVNMRAPGMGY
                     FRMPLTVWSLFITAILLLLALPVLTAALAMLLFDRMAGTSFFLPEGGGEPLLWQHMFW
                     FFGHPEVYILVLPAMGITSDLLSTFSRKPIFGYHAMAFSMIAIAGLSWIVWGHHMFIS
                     GMNPLLGTAFMMTTMVIAVPSAIKVFNWLGTLWGGSIRLTVPMLFALGFVSNFLIGGL
                     SGIFMASTPVDIFIHDTYFIVAHFHYVVAGIIFALFAAVYYWFPKMFYRQMNQPLGKI
                     HFVLTYIFFNGAFFPMHFLGVGGHMRRIYNPLQYEFITPLHDWNVFITWSALILGASQ
                     IFFLVNFFYSLFAGKKAEQNPWQANTLEWSAPTPPPHGNFEVTPTVYRGAYEYSSPEV
                     TEDWLPQDKDLGTHAAAKAH"
BASE COUNT          722 a          742 c          778 g          477 t
ORIGIN      
        1 aacggtatca gcgcagtaaa cttcgctaaa taggataacg cgccgagcag caactgtccg
       61 accaaaagcc cgccgagcaa atagcctggc cgcatcaatg ctgttgccgc gccatgttcc
      121 cgggagatcc gcagcagcaa caaaattaca tgcaccgcta ccaatccggc aaacaagaga
      181 tgagcgtcga gacgcgcgcc ggtgtggcgc agcatcgcgc caaagacgat ttgacaatag
      241 ataagcgccg tcgtaatcgc cgctaatcgg cgcagtcgcc cgccgtcgga tagcggtgct
      301 ccctgcggcg ggttacgcca ctctcgggct gtgaacagtg ccaggctcac ggtcaaggca
      361 aaaaatgcct gagcaaaaca agcatgaagt attgccagag tcggttgcag caacaccacg
      421 cgcaaaccgc cgagaacgcc ttggacgatg accaaagcca gcgccgcgac gccgagccag
      481 cgcagccatc gacgcggctc ttttagccac aacgtcagcg tgagtgcgat agtcaatagt
      541 ccgacgcagg aagcgaccag ccgatggcta tgctcgtaga agatcccacc gatcattttt
      601 gaccaggggt agagaaacat gttgtaaccg aaggttgtcg gccagtctgg aacggccagg
      661 ccgacaccaa gactagtcac caagccgccg ataaatagta acggaaacgt ggcgcaggct
      721 aggatcaccg ctaggcgatg cggccaaggt gaaaagaacg aaggatgaca ggcagaggtt
      781 gcccccgttc ctcttgattc cacgttcatg ttgccggctc tcgcttagtg ggctttggcc
      841 gcggcatgag tgccgaggtc tttatcctgc ggcagccaat cttccgtcac ttccggtgag
      901 ctatattcat aggcgccgcg gtagaccgtc ggagtcactt cgaagttgcc atggggtggc
      961 ggcgtcggcg ccgaccattc gagagtgttg gcttgccaag gattttgctc cgccttcttg
     1021 cccgcgaaca gactgtagaa gaagttcacc aggaagaaaa tctgcgatgc gccgaggatc
     1081 aaggcgctcc aggtgataaa aacattccag tcgtgcagcg gggttatgaa ttcgtactgc
     1141 agcgggttgt aaattcgccg catatggccg ccgacaccca ggaaatgcat tgggaaaaag
     1201 gcgccgttaa agaaaatgta ggtgaggaca aaatgaattt tccccaaggg ttgattcatt
     1261 tgccggtaaa acattttcgg gaaccaataa taaaccgcgg cgaacagcgc gaagatgatg
     1321 ccggcgacga cgtagtgaaa gtgggcgacg atgaagtagg tatcgtggat aaagatatcg
     1381 actggcgtcg acgccatgaa aatgccgctc aagccgccga tcaggaagtt cgagacgaat
     1441 cccaacgcga aaagcatcgg cacggtgagc cgaatcgaac cgccccaaag cgtgccgagc
     1501 cagttaaata ctttaattgc agagggcacg gcaatgacca tcgtcgtcat cataaaggcg
     1561 gtgccaagca gcggattcat cccgctgatg aacatgtggt ggccccagac gatccacgac
     1621 aggccggcga tggcaatcat cgaaaaagcc atggcgtgat aaccgaagat cggcttgcgc
     1681 gagaaggtcg acagcagatc ggaagtgatg cccatggccg gcagcacgag aatataaacc
     1741 tcaggatggc cgaaaaacca gaacatatgc tgccaaagca gcggctcgcc gccgccttcg
     1801 ggcaggaaaa aactcgtgcc ggccatgcgg tcgaagagca gcatggccaa ggcggcggtg
     1861 agcaccggca acgccagcaa caataagatc gcggtgataa atagcgacca gaccgtcaac
     1921 ggcatgcgaa aatatcccat gcccggcgcg cgcatgttga cgacggtcgt gatgtaattg
     1981 atcgcgccca tcaatgacga aatgccgttg accatcaacg cgataatcca cagatgctgg
     2041 ccccactcga cgccggtata ctgcgccttg gcggagagtg tcgcgtagcc ggtccaaccg
     2101 cccgccgccg gaccgccggg gacaaagaaa ctcgacagca tgatcacacc ggatatcgcg
     2161 ccaaaccaaa acgacaacat gttgagcttg ggaaaagcca tgtcgccggc gccaatcatc
     2221 agcggaatga gaaaattacc gaaagtgcca accatgatcg gcatgaccac aaagaaaatc
     2281 atgatcgtcg cgtgcatggt gaacaaggca ttgtaggtct gcggtggaat aatgccacca
     2341 ccattgggac cggggtaaat gtaaggttcg gagacgaagc ccagtcccgg caccggagtt
     2401 tccggccagg cgagctgcca gcgcaccagc atagcgagaa ggccgccgat caggagcatc
     2461 agcaggccga cgaagaggaa ttggcgaccg atcatcttgt gatcgagcga gaagatataa
     2521 tggctgataa aactcggctt tggatgatgt ccatgcgctc catgcgccac tgctgactcg
     2581 ctcatggctt aaaaacctcc gtagtgatgc ttcgatagta taggtatgat caaaccggct
     2641 actgcggctt ggacgctaaa ttttgcgccg cccatttctg atactcttcc ggggtatgga
     2701 cgttaagcca tcccttcat
//
LOCUS       DASQKS010000645         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1619603, whole genome shotgun sequence.
ACCESSION   DASQKS010000645 DASQKS010000000
VERSION     DASQKS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619603"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1393
                     /locus_tag="VE170_16735"
     CDS             167..1393
                     /locus_tag="VE170_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ17141.1"
                     /translation="MVSEKISVHCCIVGGGPAGMMLGYLLARAGVKVLVLEKHGDFLR
                     DFRGDTIHPSTLEVMHQLGLLDEFLKLPHQKVYELNAQVGEMRLIIADFRHLPTRCGF
                     VAFMPQWDFLNFVAEKAAPYPGFALMMDAEVNSLIEESRRVIGVRANGPKGALEVRAD
                     LVVGADGRSSIVRETAGLHVKEYGAPMDVLWFRLRRTAEDPVATMGRFDSGRIFIALN
                     RGDYWQCGYVIAKGQFDALRRQEFATFRADIVQLAPYVADTVHELRGWDDVKLLTVRV
                     DRLIDWFRPGLVCIGDAAHAMSPVGGVGINLAIQDAVAAANVLFKPLRQGQLAIDHLH
                     RIQKRREWPTRVTQGLQVAVQRRIITRVLAGTGPLTPPLPLRLISKIPFLPRIPARIV
                     GLGIRPEHVHPELLRP"
     gene            1432..1647
                     /locus_tag="VE170_16740"
     CDS             1432..1647
                     /locus_tag="VE170_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17142.1"
                     /translation="MIETEHDCPARSWGRVLNCDNVLPRDVAPALLHRTVSHTVKDPQ
                     GYKVRLTTGTGMYYDRIYVLIPDSKEE"
     gene            1650..2078
                     /locus_tag="VE170_16745"
     CDS             1650..2078
                     /locus_tag="VE170_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HYJ17143.1"
                     /translation="MAYSVKKVEVWAGEIADRPGGLADKIQSLSNAGANLEFLIARRA
                     ADKPGTGVVFVTPIKGAKQKNAAQGTGFAMTDSLHSVRVDGPDKAGLGGKMTKAIADA
                     GINLRGLSAAALGRRSVSYLAFDSAADADSAVSILRKALK"
     gene            complement(2565..3617)
                     /locus_tag="VE170_16750"
     CDS             complement(2565..3617)
                     /locus_tag="VE170_16750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ17144.1"
                     /translation="MKERALLLTLIFCFSMFLPGIAPTAGAQSDQFYKGKTIRIMVGS
                     TAGGFYDRWGRLFGKYMTKYIPGQPEFVVQNMTGAGSVIAANHVYNVGKPDGLTLVMP
                     LNGVYIDQFVGRKEVQFDMRKFRFIGSPVTEANIFYMRADAPYKSIAEIIKAKEPPKC
                     GGSGTASSDYILSKVLEETVGAKFNTVLGYAGGTEIDLAVEKNEVVCRAHSMSAHFGR
                     EPFDTWHKRGFDRHLVQTSSKKDSRAADAPTLNELFDQYKVPANSRRLAQVLLAAGDF
                     GRPMMVTPGTPPDRVKMLRDAFVKTLSDPEAIEEAKKGRMDIDPATGEELEKLVKDIF
                     ESPPDVVERVKKILAN"
     gene            complement(3937..4917)
                     /locus_tag="VE170_16755"
     CDS             complement(3937..4917)
                     /locus_tag="VE170_16755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYJ17145.1"
                     /translation="MTLSNRALVTRGTPPGELIDMAVRAEATGAVDAIWVGDSILSKP
                     RLECIPLLGAIAARTSRVKLGVACMATIAQRNPVLLALQWASLDVLSGGRTWLAACMG
                     YPASQHPMAAKELEVMGVASKERPGRLEEMIQALRIIWSDEHANFHGKYYSFEDVNLL
                     PKPAQRTCPIYIAGTPRAAQIGERGVELSLRRIARYADGWMSNQIELSMFRDHQARLR
                     QMLVEEGRDPETFKTVLYYGVSVNSDREQAFREAKAFLDAYYQKDFTRHGVEIWTACG
                     PMEHCVSCIKEFIDAGVDHVAIRPIGEDLNAQFRIYLDELLPALNAANDS"
BASE COUNT         1026 a         1423 c         1369 g         1117 t
ORIGIN      
        1 tgccagagcc gtcgcctgat tatcggcgca ggcgatcatg gcgacgctat ctggcatttc
       61 gcacggccat cttcggctca cattgacgtg gatacttgct acacaattcg ttggtcggtc
      121 attagataga ctgtacgcct gccagtttat gggttagatc taaaccatgg tgtctgagaa
      181 aatttccgtg cactgctgca tcgtgggcgg tgggccggcc ggcatgatgc tggggtatct
      241 gctggcgcga gccggcgtca aagtcttagt gctggaaaag cacggcgatt tcctccgcga
      301 ctttcgcggc gataccattc atccctcgac cctcgaggtg atgcaccagc tgggattgct
      361 cgatgagttt ctcaaactgc cgcatcagaa ggtttacgag ctcaacgctc aagtcggcga
      421 gatgcggctg atcatcgcgg acttccggca cctgccgacc cgctgcggct ttgtcgcctt
      481 catgccgcag tgggattttc tcaacttcgt cgccgaaaaa gcggcgccct atcccggttt
      541 cgcattgatg atggacgccg aggtaaacag ccttatcgag gagtcgcggc gcgtgatcgg
      601 agtgcgcgcc aatggtccca aaggcgcgct cgaagtccgc gccgatctgg tcgttggcgc
      661 cgacggccgg agctcgatcg tgcgcgaaac ggctggtttg cacgtgaagg aatacggcgc
      721 gccgatggac gttctctggt ttcggctccg tcgaactgcc gaagatcccg tggcaaccat
      781 gggcaggttc gatagcggca ggatttttat cgcgctcaat cgcggcgact attggcagtg
      841 cggttacgtc atcgccaagg gacagtttga cgcgctgcgc cggcaagaat ttgcaacttt
      901 tcgcgcggac atcgtacagc ttgcacccta cgtcgccgac accgtgcatg agctgcgcgg
      961 ctgggacgat gtaaagcttt taactgttcg cgtggaccgc ttaatcgatt ggtttagacc
     1021 cggtctggtg tgtattggcg atgccgctca cgccatgtct ccggtaggcg gcgtcggcat
     1081 caatctcgcg atccaggatg ccgtggcggc agccaatgtt cttttcaaac cgctgcgcca
     1141 gggacaactc gccatcgatc acctgcaccg gatccaaaaa cgccgcgagt ggcccacgcg
     1201 cgtgacgcaa gggttgcagg tcgccgtcca gcgccggatt attacgaggg tgttggctgg
     1261 aaccggaccg ctcacgccac cgctcccgtt gcgcttaata tctaaaattc cgtttcttcc
     1321 gcgaatcccg gctcggatcg tcggcctcgg catccgcccc gaacatgtgc atccggaact
     1381 cctacgtcca taaaaatcga acctcagaga ttcgtcacta aggatttgcc catgatcgaa
     1441 acggagcatg attgcccggc gcgatcatgg ggtcgcgtct tgaattgcga taatgtgctg
     1501 ccgcgagacg tcgccccggc tcttttacac cgaacggtga gccacacggt taaggaccca
     1561 cagggctaca aagtgcgctt gacgaccggc acgggaatgt actacgaccg catttacgtc
     1621 ttaattccag acagtaagga ggaatgacca tggcgtatag cgtaaagaaa gtcgaagttt
     1681 gggccggcga aatcgccgat cgccctggag ggctggccga caaaatacaa tccctaagta
     1741 acgccggtgc aaatcttgag tttttaatcg cgcgccgcgc cgccgataaa cctggcacag
     1801 gggtcgtatt cgtcactccg atcaaaggag caaagcagaa aaacgcggcc caagggacag
     1861 gcttcgccat gaccgatagc ctccattcgg tgcgggttga cggccccgac aaagcgggcc
     1921 ttggcgggaa aatgaccaaa gctatcgccg acgcggggat caacttacgc ggtctttcgg
     1981 cggcggcgct tgggcggcgc tccgtgagtt acttggcttt cgacagcgcg gcggacgcgg
     2041 atagcgcggt cagcatcttg agaaaggcgc tcaagtaaaa gtcttagacc gatagtttga
     2101 acaactaact ggcggcgagc caggcgtagg cgggtgcgta tctcgcgtcc gagcaacgct
     2161 tgcccatcaa tcagcgtctt ttcgtggcga gctaatgacg aagggtttgg atcaatcttt
     2221 atttctaata ttgtgccaac aggcatcagg gcctagcctt gaatcttaga caacttctcg
     2281 agtcgatgcc tcgcattgtc tgtttcgagg cgaagataag cctcgcgctt tgccgattca
     2341 tattcagttc tgaaaaaaag tgtcaggcat tgcttatttg attactgcgc tatcctttgc
     2401 ccgccgctga attctggcaa tacgtctttc aacgaagaca ctatgcctaa cgttttccgt
     2461 tatcgtcttg atcgaataac aatcaagcga gagagcgccc aggttcgttt cgccggggtt
     2521 cgagccggac ggcatccatc aaaagccgtt gcttccgata ccgctcagtt ggcgagaatt
     2581 ttcttcaccc gctcgacgac gtccggtgga gattcgaaga tgtctttaac taacttctct
     2641 agttcctcac cagtcgcagg gtcaatatcc atccggcctt ttttcgcttc ctcaatcgct
     2701 tcgggatcgc tcaaggtttt gacaaacgcg tctctcagca ttttgactcg gtcgggtggc
     2761 gtgcccggcg tcaccatcat cggccggccg aaatccccag ctgccaacag cacctgagcg
     2821 agacggcggc tgtttgccgg caccttatat tgatcaaaga gttcgttaag cgtgggcgcg
     2881 tcggcggcac gggaatcctt tttgctcgag gtctgaacca gatgccggtc gaagcctcgc
     2941 ttgtgccagg tatcgaaagg ttcccggccg aagtgggcgg acatgctgtg agcgcggcaa
     3001 accacctcgt ttttttccac cgccagatcg atctcggtgc cgccggcgta gcccaaaacc
     3061 gtgttgaatt tcgcccccac cgtctcttcc aacactttgg acaggatgta atcgctgctc
     3121 gcggtcccgc tgccgccgca cttgggcggc tctttcgctt tgatgatttc cgcgatggat
     3181 ttgtacggag cgtcggcgcg catgtaaaag atattggctt cagtcaccgg agagccaatg
     3241 aatcgaaact tacgcatgtc gaactgaact tccttccgcc cgacaaactg gtcgatgtaa
     3301 acgccgttga gcggcatcac tagggtcaga ccatctggct tcccaacgtt gtatacatgg
     3361 ttggcggcga tcacagagcc ggcaccggtc atgttttgca cgacgaactc gggctggcca
     3421 ggaatatatt tggtcatgta cttgccgaac aggcggcccc accggtcata aaatccgccg
     3481 gccgtcgagc ccaccatgat gcgaatggtc tttcccttat aaaactgatc gctctgcgct
     3541 ccggctgtgg gagcgatgcc cggaagaaac attgaaaaac agaaaatcag cgtaagcaaa
     3601 agcgctcgtt ctttcatagc aaactccttt cgctgtcttg atcatctgcc ctagtgacgg
     3661 ttcaccccct gcgcgaccta tttatccact cactcctccc gccgttcgtc gactatctcg
     3721 aagcccggtt tgtgtcaagc ccaattcgca tggtgcgcat ggggtcagcg aaacctgtca
     3781 atgggatggg acacgtccga ttcagaaatt cacagaacga tgatgggctg cgtcttgctt
     3841 tgcgacaagg tgtaattgca cgacttggct ccaggtctgt tacctgcaac cgtcggaaga
     3901 cacgctttac ccgcaacagc gtcgccacgc cacgaactaa gaatcgtttg ccgcgttcaa
     3961 agcgggcaga agttcatcca aatagatgcg gaactgcgcg tttagatctt cgccgatcgg
     4021 gcgaatcgct acgtgatcga cgccggcatc gatgaattct ttgatgcagc tgacgcagtg
     4081 ttccatcggt ccgcaggcgg tccagatttc gacgccgtgc cgggtaaagt ctttttgata
     4141 ataggcgtcg agaaaggctt tggcttcgcg aaatgcctgt tcccgatcgc tgttcacgga
     4201 tacgccgtaa tacaaaaccg ttttgaaggt ttctggatcg cgcccctcct caaccaacat
     4261 ttgccgcagt ctggcttggt gatctcgaaa catcgagagc tcgatttggt tgctcatcca
     4321 accatcggca tagcgcgcaa tgcgccgtaa agagagctcg acgccgcgct cgccgatctg
     4381 cgccgcgcgc ggtgtgccgg cgatataaat cggacaagtc cgttgcgcgg gcttcggtaa
     4441 cagattcacg tcttcgaatg aatagtattt gccgtgaaag tttgcgtgtt cgtccgacca
     4501 gataatccgc agagcctgaa tcatctcttc cagccgtccg ggccgctctt tactcgccac
     4561 gcccatgact tcgagctctt tggccgccat aggatgctgg ctcgccgggt aacccatgca
     4621 cgcggccagc catgtgcgtc cgcctgacag aacgtcgagg ctggcccact gcaatgcgag
     4681 cagcacggga ttccgctggg ctatcgtcgc catgcaggcc acgcccagtt tcacacgcga
     4741 agtccgcgcg gcgatggcgc ccaataaagg gatgcattcc agccgcggct tgctcaaaat
     4801 actatcgccg acccatatcg catcgaccgc gccggtcgcc tcggccctta ctgccatgtc
     4861 gataagttcc ccgggcgggg ttccgcgcgt aaccaatgcg cggttggata atgtcaaacc
     4921 aaatgtcgtt ttctg
//
LOCUS       DASQKS010000646         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5410373, whole genome shotgun sequence.
ACCESSION   DASQKS010000646 DASQKS010000000
VERSION     DASQKS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5410373"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VE170_16760"
     CDS             <1..786
                     /locus_tag="VE170_16760"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ17146.1"
                     /translation="TLSGIKTYDRVSPKAGLVYTPVKNLSFSFSYSEGVRIPTVDELF
                     AQGPFGSNPDLKAMTSRNFELGAKARLQDWLDASLALFYTPVRDEILFIVTDPLLFFG
                     RNENISRTLRRGIELSLKARYQKWLDAFINYTAMKATFETDVLLFSGQVRKGDELPLV
                     PRHRVGVGVNTYPIEGLTVSLFGNYVGSQFMQSDEPNQAKKVADYFVLNSRVAYQWKQ
                     WTGYINFNNLTNRKYSTSGILVTEPFRVPAPTFNVFAGLSFKY"
     gene            847..1752
                     /locus_tag="VE170_16765"
     CDS             847..1752
                     /locus_tag="VE170_16765"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HYJ17147.1"
                     /translation="MIYALATLLSSVALAAAGSRTIVDDLGFKVSVKRDIRRVVSLVP
                     TNSEMVCLLDCQRLKGGTRYDQFPEELVSRIQGKQVAIIGGGFDANLEKIVHLQPDLI
                     LTNGPTQQRFAVPLKNLGYAVVSLWPRDMAGLVKDFLLLGELLGQDHKARSFIDDMQR
                     RFQAIASRAQAMPKKRAYLQMWTEPLITVGKASFTDWLVSAAGGSNIFGDMSFDSGQV
                     GLESLIQRNPEVLIFLAEQAPFVKTLPRRAGWSSIQGVRDGHFCFIDEPDIRRSIMFI
                     DGLAKLQSCLLSKPVHGQRSSMGFP"
     gene            1800..2723
                     /locus_tag="VE170_16770"
     CDS             1800..2723
                     /locus_tag="VE170_16770"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ17148.1"
                     /translation="MILIGLSIGPVPISLFAMSPTDKEILFNLRLPRVLSAFLVGGGL
                     ALAGAIFQGLFRNPIADSYLLGVSSGASLGAAAAIVLKLASPLASFAAVSVFAFAGSL
                     AALALVFYFGHRQGSFSIFRLLLAGLGVGLFLSSLVAIFMLMAGEELKALIFFFLGGF
                     SSSSWDTTWVSLACIPLAALASCYLAPELNMLLLGEETAQSSGVRTARIQLLFAILAA
                     LLTSISVSVAGLIGFVGLIVPHMIRLSVGADHRTLLPLAFFSGGIFLVLCDAAARTVM
                     APAELPVGIVTSLVGVPVFLYMIRKESSDLG"
     gene            2728..3525
                     /locus_tag="VE170_16775"
     CDS             2728..3525
                     /locus_tag="VE170_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ17149.1"
                     /translation="MTSASALEVRNISFGYNGGLLLNNLSFAIGAGELIALIGPNGSG
                     KTTLLKILLGLLAPSSGTVTLHGQALSTYEPKARAKTIAYVSQQPAQSFPLTVRELVS
                     LGRYPHGARGKESSADQLAIAKALERTAAIELGARQFNTLSGGEKQKVLIARALAQSA
                     RVLLLDEPTLHLDLKFQLQILSALRRLCVEEQITVVTVLHDVNQASLFADRALLLSEG
                     ALGGFGPVAEVINAASIKALLGVDMTAIAGTVSHPRYFVPSDPFSRQ"
     gene            3545..>3978
                     /locus_tag="VE170_16780"
     CDS             3545..>3978
                     /locus_tag="VE170_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HYJ17150.1"
                     /translation="MPAKTLMVQGTGSSVGKSILVAALCRIFKQQGLRVAPFKAQNMS
                     LNSFVTAAGDEIGRAQVVQAEAAGIAPTVAMNPVLLKPESDCNAQIIVRGKPLTRISA
                     AKFPQLKAELWCAITESLEQLRGAYDVVVIEGAGSPAEINLK"
BASE COUNT          801 a         1149 c         1090 g          938 t
ORIGIN      
        1 actcttagcg gtataaaaac ctacgaccgc gtcagcccaa aggccggact cgtatatacg
       61 ccggtcaaga atttgagctt ttcatttagc tactcggagg gagtgcgaat tccaacggtc
      121 gacgaactat tcgcgcaggg accgttcggc tccaatccgg atttgaaagc gatgaccagc
      181 cgtaatttcg agcttggcgc caaggcgagg ttgcaagatt ggctcgacgc atcactggcg
      241 ctgttctaca cccccgtacg tgatgaaatt ctttttatcg tcaccgaccc gcttttattc
      301 ttcgggcgca atgagaatat ttcccgcacc ttgcgccgcg gcatcgagct ctcgctaaaa
      361 gctcgctatc aaaagtggct cgatgctttt atcaattaca ccgccatgaa agccaccttt
      421 gaaaccgatg tcctgctctt ttccggccag gtgagaaaag gcgacgagct gcccctcgtg
      481 ccgcgccatc gagtcggcgt aggcgtcaat acctacccca tcgaaggcct aaccgtatcg
      541 ctgttcggga attatgtcgg ctcgcaattc atgcagagtg acgagcccaa tcaagccaag
      601 aaggtcgccg actactttgt tcttaatagc cgtgtcgcct accagtggaa acagtggacc
      661 ggatacatca atttcaacaa tttgaccaac cgaaaatatt cgacctccgg tatattggtc
      721 accgaaccgt tccgtgttcc cgcgccgacg tttaacgtct tcgcaggtct gtcgttcaag
      781 tactagatga aagtattctt gaacgcgcat gaggccgtgt aagatatgca gtgtccgggg
      841 ataatattga tttacgctct cgcgacactg ttaagttccg tcgctctggc cgcggccgga
      901 agcagaacca tcgttgacga tctaggtttc aaagtcagtg tgaaacgcga tatccgcagg
      961 gttgtgtcgc tggtgccgac caattcggag atggtgtgtt tgttggattg ccagcgattg
     1021 aaaggcggca cccgttacga tcagtttccc gaggaattgg ttagtagaat ccagggcaaa
     1081 caggtcgcca tcatcggcgg cggcttcgac gccaatttgg agaaaattgt ccacctccaa
     1141 cccgatctca tcctcaccaa cgggcccacc cagcagcgct tcgccgtgcc gctaaagaat
     1201 cttggctatg cggttgtcag tctgtggccg cgtgacatgg ccgggctggt gaaagatttc
     1261 ttgcttctcg gcgagctcct cgggcaggat cataaagcgc ggagctttat cgacgacatg
     1321 cagcgacgtt ttcaggcgat tgcaagcaga gctcaggcta tgccaaaaaa gcgcgcctat
     1381 ttgcagatgt ggaccgagcc gttgatcacc gtcggcaaag cttcgttcac cgactggctc
     1441 gtcagcgccg ctggtggcag caatatattc ggcgacatgt cgtttgactc ggggcaggtc
     1501 ggcttggaat cgctgatcca gaggaatcct gaagtgctga tctttctcgc ggaacaggca
     1561 ccctttgtaa aaacgctccc gcgccgtgct ggctggtcgt cgatccaggg cgtgcgcgac
     1621 ggccactttt gtttcatcga cgagcctgac attcgtcgca gcattatgtt catcgacggc
     1681 ctggcaaagc tgcaaagctg cctcctcagt aaacccgtac acggacaacg gtcatcaatg
     1741 gggtttccgt gaaccgggaa agtttgcatg tatggcttct ctccatatgc accttggccg
     1801 tgatcctgat tggcttgagc atcggaccgg tgccgatctc gttatttgcc atgtctccta
     1861 cagataaaga aatcttgttc aacctacgcc tgccgcgcgt cctatcggcc ttcctggtgg
     1921 gtggcggctt ggccctggcc ggcgcgatct ttcaaggtct gtttcgtaac ccgatcgcgg
     1981 atagctacct gctcggcgtc tcctccggcg cgtcattggg cgcagccgcg gcgatcgtgt
     2041 tgaagcttgc ctcgccgctg gcaagcttcg ccgccgtaag tgtctttgct ttcgccggtt
     2101 cgctggccgc cctcgctttg gtcttttact tcggccatcg ccaaggcagc ttctcgatct
     2161 ttcgtttgct gctcgcgggt ttgggcgttg gcctgtttct ttcatcgctg gtcgccatct
     2221 tcatgctgat ggccggcgaa gaactgaaag cgctgatatt tttttttctt ggcggctttt
     2281 ccagttccag ctgggatacc acctgggttt ccctggcgtg catacccctc gccgcgctcg
     2341 cctcctgtta cctcgcgccg gagttaaata tgctgctgct cggcgaggag acggcgcaaa
     2401 gcagcggggt tcgcaccgct cgcattcaat tactcttcgc cattctcgcg gcgttgttga
     2461 cgtcgatttc ggtatcggtg gcgggcttga tcggcttcgt cggtttgatt gtgccgcata
     2521 tgatccgctt gagtgtcggt gccgatcata gaactttgtt gccgctggca tttttttccg
     2581 gcgggatatt cctcgtgctt tgtgacgccg cagcgcgcac cgtcatggcg ccggcggaac
     2641 taccggtcgg catcgtgacc tcactggttg gtgtaccggt ttttctgtat atgatccgta
     2701 aagagtccag cgatctcggt tgataatatg acatcggcga gcgcactcga agtccgcaac
     2761 atctccttcg gctacaacgg cggcctgctg ttgaataatc tttcgtttgc catcggcgcc
     2821 ggtgaactta tcgccctcat cggccccaac gggtcgggca aaaccacgct gctcaagatc
     2881 ttgctcggcc tgctcgcgcc gagctccggc acggtgacac tgcacggcca agccctgtcg
     2941 acttatgaac ccaaagcgcg agcgaaaacc atagcctatg tttcccagca acctgcgcaa
     3001 agctttccct tgaccgtacg cgagctggta tccctcggcc gttatcccca tggcgcgcgc
     3061 ggcaaagaat cgagcgccga ccaactcgcc atcgccaagg cgttggaaag aaccgcggcg
     3121 atcgaactag gcgcccggca gttcaacacg ctgagcggcg gcgaaaagca aaaagtcttg
     3181 atcgcccgcg ctttggctca atcagcccgg gtcttattgc tcgacgagcc gacgctgcat
     3241 ctcgatctca agtttcaatt acaaattctc tcggcgttga gacggctatg cgtggaagag
     3301 caaattaccg tggtcaccgt gctgcacgat gtcaatcagg cgtcgttgtt cgccgaccgc
     3361 gcgctgctat tgagcgaggg cgcgctcggt ggtttcggac cggtcgcgga agttatcaac
     3421 gcagcgagca taaaagcgct actgggcgtc gacatgacag caatcgccgg gaccgtttcg
     3481 cacccgcgtt atttcgtccc gagcgatcca ttttctcggc aatagccggc ccggacacaa
     3541 tcacatgccc gccaaaacac tcatggtcca aggaaccggc tcatcggtcg gcaaaagcat
     3601 tctcgttgcc gcattgtgcc gcatttttaa acagcagggc ttgagagttg cgccgttcaa
     3661 ggcgcaaaac atgtcgctca actcgttcgt cacggcggcg ggcgacgaga tcggccgcgc
     3721 ccaagtcgtt caagccgaag cggcggggat agcgccgacg gttgcgatga atccggtatt
     3781 gttaaagccg gaaagcgatt gcaacgcaca gattatcgtc cgcggcaaac cgttgacgcg
     3841 catatccgcg gcaaaatttc cccaactcaa agcggaactc tggtgcgcga tcacggaatc
     3901 tctcgaacaa ctgcgaggcg cttatgatgt cgtcgtcatc gagggcgccg gtagtcccgc
     3961 tgagatcaat ctcaaaga
//
LOCUS       DASQKS010000647         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5410782, whole genome shotgun sequence.
ACCESSION   DASQKS010000647 DASQKS010000000
VERSION     DASQKS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5410782"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(783..857)
                     /locus_tag="VE170_16785"
     tRNA            complement(783..857)
                     /locus_tag="VE170_16785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Gly,seq:ccc)
     gene            complement(984..1787)
                     /locus_tag="VE170_16790"
     CDS             complement(984..1787)
                     /locus_tag="VE170_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYJ17151.1"
                     /translation="MRCEQAQELITARLDNELSADEHGAIEAHLHTCQRCQRALTAES
                     RLKQQLKLTRQEVTAPAALRRELESAIVSRRTAVTGAGRFVSWFGLRNWRPAVAVALL
                     IIAIAALFLFRSPQPDLAMAVLDSHRDLLSGKTALVRYDDATRLRQELSRAVRDRFAP
                     IALDLSMMRLYPVSGFVQKIGDRDVLVTVYQGAGPAITCFTLLGSESDAPEGAQHFYD
                     SDMKLNFYSFSQDGINAVLHSEGDIICILVSKMSPADILALLRGKSAHA"
     gene            complement(1796..2416)
                     /locus_tag="VE170_16795"
     CDS             complement(1796..2416)
                     /locus_tag="VE170_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ17152.1"
                     /translation="MDTPYTLGYTGWAVSATNSRNPAPDSFATEVMTYLDHLYRVALH
                     LVRNADEAKDCVQETCTRALSSQGQFLPGSNMKAWLTRILYNFFYDSYARNKRTIAAE
                     DLLDGTDAVDFWEAHAGDGPSPESQILDRELGAKIGAALKQIPEEFRAPILLVDMGDF
                     TYAEAAEVLSCPIGTVRSRLSRGRRLLQKQLGGYLGRTGTASPEEG"
     gene            2519..3463
                     /locus_tag="VE170_16800"
     CDS             2519..3463
                     /locus_tag="VE170_16800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ17153.1"
                     /translation="MKYRTLGKTGLRVSVIGLGTMVHAGHFGPMKDSESLSAIETALD
                     LGINFIDTSDAYGAGYSETLLGNALKGKRDQVVIATKGGNVMVGPNRGKRNFDPDYIS
                     RVMEESLQRLQIDTIDLYQLHNPTVEVIERGAVWEVLERAKQAGKIRHYGVSINSMEE
                     GIAAVNDGRAETIQVEYNLLAQEPAEKLLPLAYQANIGIIARVPLRRGVLTGKMSVAD
                     EQRFQGEDLRARSFKGEAFAKELAKADELRFLVHDTAKTLGQAAIAFCIAHPAVSVVV
                     PGARNAEQMRENGAAANIEISAADLARIADLWRRNRAG"
     gene            3487..>4215
                     /locus_tag="VE170_16805"
     CDS             3487..>4215
                     /locus_tag="VE170_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ17154.1"
                     /translation="MERTLAAWPNNTKLVVMVTIMFEVWSDGKAPPYSPMTTSLKPGT
                     PDLLGISWSQYGGKTGIWRFLRILDDTEIKSTVCLNAKGAELFPDAVNAAHRRGHEIA
                     GHSYTQDTVLPYLSALEERELIDRCKRIITDAVGAPPVGWFSPVAAPTVHTAQFLAEA
                     GFIWHGDYNDSDLPYTVETGKGRLVAFAHSDFTDNRTLRSSPRDFYNVYKDTFDYLYR
                     HEAPSLLNLTMHCHFGGRPLMSAML"
BASE COUNT          909 a         1238 c         1198 g          870 t
ORIGIN      
        1 gatgatttga tcgttgcttg gggtgctggg aattttggat tggggataac gggacggaat
       61 gaaaaaggcc cggcttacgc ttggaatatt ctttagggtc acttaccgtt cgtattcgag
      121 atttggtttt ccgtcgtcga tccacttttt ggcgaacagg ggccctccat ttccgcctcc
      181 ttcgctgtca gaaaactgca cttgaagtac cggtccatcc agagcttaac acgaggcttg
      241 ctaccgtcgc tccagttaat ttgcacgatg ggtttccgtt cgaggccttt agggacgcgc
      301 ccagcatgag cggattctat tgtcctggta agtctcttcc attagtcctc ctgccgtccg
      361 cgctccacgt aaactgtcgc gttgccgtcc aaatggttcg cgtaaaaaaa attccggggg
      421 cttcacttta gcgcactcag tattattttg aatttgtagt tggcggcttc tgcttttgac
      481 ggagaggtta agcaatggtg caaccgatgc caggtctatc acacatcaga aaagccatcc
      541 gttaccgaag cctcgctcag cctgaagcta tgcgatatct gcgaagagaa cccgtgccga
      601 tgcaatgaga tttacttatg aaggcgtccg acgttcatca catgggtcgt aagctgtcgg
      661 tcaaacgaag gggcacacga catatgtcga ggccactcta attttgcctt ttgagtatta
      721 cttcgttgcg aggcttaccg attcgccgta gcaccatcat agtcaatcga tgctaagcaa
      781 tttggagcgg gcggcgggga tcgaacccgc gacgtccagc ttgggaagct ggcattctac
      841 cgctgaatta cgcccgccca cataaaggta gtgaagctgt caaactgtag gtaatcgcta
      901 ccaagttgtc aacgcgtaga gagttaaatc atctccgctg tccaggaggg aacatttgac
      961 cgtcacggat cgacgatcgg attttaggca tgcgccgact taccgcgcag cagtgcgagg
     1021 atgtcggcgg gcgacatttt tgaaaccaaa atgcagatga tatcgccttc gctgtgcagc
     1081 acggcgttga tgccatcctg tgaaaaggaa tagaaattga gcttcatgtc gctatcgtag
     1141 aagtgctggg cgccctcggg cgcatccgac tcggaaccca gtaaagtaaa gcaagtgatc
     1201 gccggcccgg ctccctggta gacggtgacg agcacgtcac gatcgccaat cttttgcacg
     1261 aagcccgaga ccggatagag tctcatcatc gacagatcta aagcgatggg cgcgaagcgg
     1321 tcgcgcaccg cccgtgacag ctcctggcgc agccgagtgg cgtcgtcgta acgcaccaat
     1381 gcggtcttgc cgctgagcag atcgcgatgg ctgtcgagca ccgccatggc aagatccggt
     1441 tgcggcgacc tgaaaaggaa aagcgccgca atggcgatga ttagcagcgc cacggcaacc
     1501 gccggccgcc aattgcgcaa accaaaccac gagacgaaac ggcctgcgcc tgtcactgct
     1561 gtgcgccgcg aaacgattgc gctttcgagc tcgcggcgca aggcggcggg cgcggtcact
     1621 tcctggcggg tcagcttgag ctgttgtttg agccggcttt ccgcagtcag cgcccgttgg
     1681 cagcgctggc acgtgtgcag atgcgcctcg atggcgccgt gctcatcggc gctgagctca
     1741 ttatctaggc gagccgtgat cagctcttgt gcctgctcgc aacgcatggg ttgatctatc
     1801 cttcttccgg acttgccgtc ccggtcctgc ccaaataacc gcccaactgt ttttgcaaaa
     1861 gtctgcgccc ccgtgacaaa cgcgagcgca ccgtgccgat cggacaggac aggacttcgg
     1921 ccgcttcggc gtaagtaaaa tcgcccatgt ccacgagcaa gatcggcgcg cgaaactctt
     1981 cggggatttg cttcagtgcg gcgccgatct tggcacccaa ctctcgatcc aatatctggc
     2041 tctccggact gggaccgtcg ccggcgtggg cttcccaaaa gtccaccgcg tcggtgccgt
     2101 cgagaaggtc ttccgcggcg atggtgcgct tgttgcgggc gtaagagtcg tagaaaaaat
     2161 tatagaggat gcgggtcagc caggccttca tattactgcc gggcaaaaac tgcccctgcg
     2221 aactcaacgc ccgcgtgcac gtctcctgga cgcaatcctt ggcctcgtcg gcgttgcgca
     2281 ccagatgcaa cgcgacgcga tagagatggt ccagataggt catgacctcc gtggcgaaac
     2341 tatcgggtgc agggttacgg ctgttggtgg cgctcacggc ccagccagtg tagccaagag
     2401 tatagggggt gtccaatcgc gcgcctcggt taagtcaacg aagcaaccga aacaaaagtt
     2461 cccccgagcc acggcgaacg cttgcggagc caggcgtggc tgtcctatgt tgacggccat
     2521 gaaataccgc acactcggca aaactggcct gcgtgtcagc gtcatcggcc ttggcaccat
     2581 ggtccatgcc ggccatttcg gccccatgaa agactcagaa tccttgagcg ccatcgaaac
     2641 ggctctcgat ctcggcatca atttcatcga tacctccgac gcctacggcg ccggctattc
     2701 ggagacgttg ctgggcaacg cgctcaaagg caaacgcgat caagttgtta tcgccaccaa
     2761 gggcggcaat gtcatggtcg gccccaaccg cggtaaacga aacttcgatc ccgactacat
     2821 cagcagggtg atggaggaaa gcctccaacg attgcaaatc gacaccatcg atctttacca
     2881 actgcacaat ccgacggtgg aagtcatcga gcgcggcgcg gtttgggagg ttttggaaag
     2941 ggcgaaacaa gccggcaaaa tccgccacta cggcgtgtcg atcaactcca tggaagaagg
     3001 cattgccgcg gttaatgacg gccgggccga aacgattcaa gtcgaataca acctgcttgc
     3061 tcaggagccg gcggaaaaac ttctgccgct ggcgtaccag gcaaatatcg gcatcatcgc
     3121 tcgcgtgccg ctgcgccgcg gcgttctgac cggcaagatg agcgtcgccg acgaacaaag
     3181 atttcaaggc gaggatttgc gcgcgcgcag tttcaagggc gaggcattcg ccaaagagct
     3241 ggcgaaggcc gatgagctgc gctttctcgt ccatgacacg gcgaaaacct taggtcaggc
     3301 ggcgatcgct ttttgcatcg cccatcccgc cgtcagcgtc gtcgtccccg gcgcgcgcaa
     3361 tgccgagcaa atgcgtgaaa acggcgccgc cgccaacatt gaaatttccg ccgccgacct
     3421 ggcgcgcatc gccgatctct ggcgccgcaa ccgcgccggc tgaattttct tgagtcggag
     3481 caatccatgg aacggactct cgccgcttgg cccaacaaca caaaactcgt agtcatggta
     3541 accatcatgt tcgaggtctg gtccgacggc aaagcgccgc cttactcgcc gatgaccacg
     3601 tcgctcaaac cgggcacgcc ggatttgctg gggatatcct ggtcgcaata tggcggcaag
     3661 accggcatct ggcgttttct gcgcatcttg gacgacacgg agatcaaatc gacggtctgc
     3721 ttgaatgcca aaggcgccga gctgtttccc gacgcggtca atgcagcgca ccgccgcggc
     3781 catgaaatcg ccggccattc ctacacgcag gatactgtcc tgccctatct gtcagcgctg
     3841 gaagagcgcg agctgattga tcgttgcaaa cgcatcatca ccgacgcggt cggcgcccca
     3901 ccggtcggct ggtttagccc ggtcgctgcg ccgacggtgc acacggcgca gtttctcgcc
     3961 gaagcaggct tcatatggca tggcgattat aacgacagcg atctgcccta tacggtggaa
     4021 accggcaaag gccggctcgt cgcgttcgcc cacagcgact tcaccgacaa tcgcaccctg
     4081 cgctccagcc cgcgcgattt ttataacgtc tacaaagata ccttcgacta tctctaccgg
     4141 catgaagcgc cgtcactgct caatctaacg atgcactgtc acttcggcgg ccggccgctg
     4201 atgtcggcga tgctt
//
LOCUS       DASQKS010000648         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3738501, whole genome shotgun sequence.
ACCESSION   DASQKS010000648 DASQKS010000000
VERSION     DASQKS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738501"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VE170_16810"
     CDS             <1..252
                     /locus_tag="VE170_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ17155.1"
                     /translation="GTFAMASSIIAEASLSFLGVGVPPHIPTWGTMLADARIYISTAW
                     WLPLFPGLCIFITVLGINLLGDALRDILDPRLKRTGSGL"
     gene            419..1822
                     /gene="pyk"
                     /locus_tag="VE170_16815"
     CDS             419..1822
                     /gene="pyk"
                     /locus_tag="VE170_16815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYJ17156.1"
                     /translation="MRRTKIVCTIGPASSAEKMIEGLIRAGMNVARLNFSHGGYTSHR
                     RVIRTIRKLERKLATPVAILQDLPGPKIRIGAIAGDRVRLQTRGRLVLTTRKILGNEH
                     AVPVDFSGLAHAVKKGDPILLGDGEIELEALQVGKNEIQCRIIVGGILGSHKGIHFPR
                     SSLNIRALTAEDKKDLAFGIANKVDLVALSFVRNSQDILYARREMHKLGANIPLIAKI
                     EKHEALDNLDSILQTVDGIMVARGDLGLEIAQQRIPKVQKHMIREANHLGKPVITATQ
                     MLRSMVWNPRPTRAEVADIANAILDGTDALMLSEETAAGDYPLEAVRIMAQVAEETET
                     ILEPRQRFTGLPKTIPEAISVSAISLARDLEVKAFLIPTTSGSTARLIARYRPAQPII
                     AVSPDRQTVKMLCLVWGVYPQPARGFKNTDEMVRVLQEKALASGIVKRGDLVAITAGL
                     PLHQSGTTNMITAKVIE"
     gene            1938..>2867
                     /locus_tag="VE170_16820"
     CDS             1938..>2867
                     /locus_tag="VE170_16820"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17157.1"
                     /translation="MSEVLKQFEKELESKNFKGYWQNIQGDAARQPVASFDACHWKGK
                     DLFAAMEKAGDVVGLDVSFRRVIQLWNPALKNGTSRTLVLNLQLLKPGENAGSHRHIA
                     GAIRFILKGHGTRLIVEGESFEIGEGDFVTTPSWTWHDHENRGENMMWLDGLDAPLVR
                     LLETDFHEPDPRKKQTVTRPDGYTLATTGPLRPAWVKPNAIQPPAFCYKWADTESALN
                     KVGEQPGDPFNGIVLDYANPLNGGPTLPTMNCQIHMLRAGEKTKGHRHTSSDIYHVWR
                     GSGVSYVGDKRFEWEQGDSFVVPLWQNHRHENTS"
BASE COUNT          639 a          890 c          782 g          556 t
ORIGIN      
        1 ggcaccttcg cgatggcgtc gtcgatcatc gccgaagcaa gcctgagctt tctcggcgtc
       61 ggcgtcccgc cgcatatccc aacctggggc accatgctcg ccgacgcgcg catttatatc
      121 agcacggctt ggtggctgcc gctgtttccc ggcctctgta tcttcatcac ggtgttgggc
      181 atcaatctcc tgggtgacgc tctgcgtgac atcctcgatc cgagactgaa gcgaaccggg
      241 tcgggactgt gaagggcaat actgcaagca tggagtgatg gagtaccggg atcggaagcc
      301 ccaacactcc agtactccaa tactccatca ctccgttctt ttcgtcactc cattcatcca
      361 atcccttctt cctcccactc gcgttgattc ccccacggtc tcgtgtaaat tgatcggaat
      421 gcgccgcaca aaaattgttt gcaccattgg ccctgccagc tcagccgaga aaatgatcga
      481 aggtttgatc cgcgccggca tgaacgtcgc ccggctaaac ttcagtcatg ggggttatac
      541 ctcgcatcgc cgggtgattc ggacgatccg caagctcgaa cgtaaactcg ctacgccggt
      601 ggcaatcctg caagatctgc cggggccgaa gattcgcatc ggcgccatag ccggcgaccg
      661 tgtgcgtttg caaacccgcg gccgcctggt cctgacgacc cgcaaaattc ttggcaatga
      721 gcatgccgtg ccggtggatt tttcgggtct ggctcatgca gtgaaaaaag gcgatccgat
      781 tctgctcggc gacggcgaga tcgagctcga agcgctgcaa gtcggcaaga acgagatcca
      841 atgccgcatc atcgtcggcg gcatcttggg ttcgcacaag ggcattcact ttccgcgcag
      901 ctcgctcaac attcgcgccc tgaccgccga agacaaaaaa gacctggcgt ttggcatcgc
      961 caacaaggtc gacctggtgg cattgtcctt cgtacgcaac agccaggata ttctctacgc
     1021 ccgccgcgag atgcataagc tcggcgccaa tatcccactg atcgccaaga tcgaaaaaca
     1081 cgaagccttg gacaacctcg acagcatttt gcaaaccgtc gacggcatca tggtggcacg
     1141 cggcgacttg ggactggaga ttgcccaaca gcgcataccg aaggtgcaaa aacatatgat
     1201 tcgcgaagcc aatcatttgg gcaagccggt catcaccgcg acccagatgc tgcgctcgat
     1261 ggtgtggaat ccgcggccga cgcgagcgga ggtcgccgac attgccaatg cgattctcga
     1321 cggcaccgac gcgctcatgc tgtccgaaga aaccgctgcc ggcgattatc cgttggaagc
     1381 ggtgcggatc atggcccaag tcgccgagga aaccgaaacc attctcgaac cgcgccaacg
     1441 attcaccggc ctgcccaaga ccattcccga agcgattagt gtcagcgcga tctctttggc
     1501 ccgcgatctg gaagtcaaag cgtttctgat tccgacgacc tccggcagca cggcgcgcct
     1561 gatcgcgcgc taccggccgg cgcaaccgat cattgccgtg agccccgatc gccaaactgt
     1621 caaaatgctc tgcctggtct ggggagtcta tccacagccg gcccggggtt ttaagaacac
     1681 cgatgaaatg gtccgcgtgc tgcaagaaaa agcgctcgct tccggcatcg tcaaacgcgg
     1741 tgatctggtc gccatcaccg caggcttacc gctgcaccag tccggcacca ccaacatgat
     1801 caccgccaag gtgatcgaat aaacgcggtt tttttcactt cagagtgcat gaaggcgcgg
     1861 gaaccattgc cgtcatcgtc ttgcaacccg cgctcgaatc gcgctagata agaccagatt
     1921 tagctggagg tgacgctatg tcggaagtac tgaaacaatt cgagaaagag ctggaaagta
     1981 aaaacttcaa aggctactgg caaaacattc agggcgatgc cgcacgccag ccggtggcgt
     2041 ctttcgacgc ctgccactgg aaaggcaagg atctcttcgc cgccatggaa aaagccggcg
     2101 atgtcgtcgg cctcgatgtg tcattccgcc gcgtcatcca gctgtggaat ccagctctga
     2161 aaaacggcac atcgcgcact ctggtgttga atctgcagtt gctcaagccc ggtgagaacg
     2221 cgggctcgca ccgccatatc gccggcgcga tccgatttat tttgaaaggc catggcaccc
     2281 ggttgatcgt cgagggcgaa tccttcgaga tcggcgaagg cgatttcgtc accacgccga
     2341 gctggacttg gcacgatcat gaaaatcgcg gcgaaaacat gatgtggctc gacggtctcg
     2401 acgcgccgtt ggtgcgacta ctcgagaccg actttcatga acccgatccg cgcaagaaac
     2461 aaaccgtgac ccggcccgac ggctacacgc tcgccaccac cggcccgcta cgcccggcgt
     2521 gggtcaaacc gaatgctatt caaccgccgg ccttttgcta caaatgggcc gacaccgaaa
     2581 gcgccttgaa taaagtcggc gagcagcccg gcgacccgtt caatggcatc gtcctcgact
     2641 atgccaatcc cttgaacggc ggtccgaccc tgccgactat gaactgccag attcatatgc
     2701 tccgcgccgg cgaaaaaacc aaaggccatc gccataccag ctcggatatc tatcatgtct
     2761 ggcgtggcag cggcgtcagt tatgtcggcg acaagcgctt cgaatgggag caaggcgatt
     2821 ccttcgtcgt gccgctctgg caaaaccacc gccatgagaa cacctcg
//
LOCUS       DASQKS010000649         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4979004, whole genome shotgun sequence.
ACCESSION   DASQKS010000649 DASQKS010000000
VERSION     DASQKS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4979004"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VE170_16825"
     CDS             <1..636
                     /locus_tag="VE170_16825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ17158.1"
                     /translation="HIVAPDLRGHGESDKPQTGYRLRDFTEDIRQLIKNLELTKPAYV
                     GHSWGGNIGTILAADSPALISRAFLEDPVYWRMIHAFMTSLPGALARRAKPGAEIRAE
                     AQQKGLSKADENLEVYKAHHFSPHALTRLQTDNRDWAIEFESYFKRINVPTTILVADH
                     KAGGAIMREELTYLQTLAPPQVKLEFWENVGHGMKAAKPAEYNRALEIWLG"
     gene            complement(653..1780)
                     /locus_tag="VE170_16830"
     CDS             complement(653..1780)
                     /locus_tag="VE170_16830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HYJ17159.1"
                     /translation="MSLVIGPCWWTIYRYQSPSCSGYKPDFISLYTGSVLVTSDPSAL
                     YDLERQRQVQQPIDPSRGSWVLPFFYPPFFALALAPLAGFSFSVAFALMTLVNIVLLA
                     LALSVLMRQLEFNFAQKKWLVLATVCNYGVYYAVLQAQTSFLALLLLVLFVSAFVDNR
                     QRRAGWWCGLLLFKPPLLVVPALLLLAKKSWRGISVLLLVILGLGAISYFMIGLEGLR
                     AYLDISQRATAGEATLSIRPDRMHNLRALAYFFAAPAWRDYFWYCLTAGVITVVAIQC
                     RRPLGLEADSFPAWVKILIGIILIAPHFHDHDMTLFTLPAAFILKIGGDEVPLCVALT
                     LVLVGALPLINTLVFPHLPPLVPLAGLLYLLPGFRLGTRSE"
     gene            complement(1907..2734)
                     /locus_tag="VE170_16835"
     CDS             complement(1907..2734)
                     /locus_tag="VE170_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ17160.1"
                     /translation="MPNVKLPTGVDFYYETHGNGEPLIFVPSTAYSGEVWKPWQMPLA
                     SSLQLIFHDPRGCGRSVPTQSVYTIEQMALDIVAMMDHLKLPAAHFIGHSMGGRIALS
                     LAQNFPGKVKSLIMAASGSGTAGRAGSDCVPGLPHRMVYDMVEMGFEKFIYDEIVESD
                     TFFTKDFRDQHRDQVETFFKVAWATHAKLDPFIHLCIARHNFEGTHRLGDVQAPTLVL
                     IGEADTVGSNHRAQAQVLMERIRGAEMKMLKGQSHGFFWQAPEETNAVILEWVKKHG"
     gene            complement(2855..>3507)
                     /locus_tag="VE170_16840"
     CDS             complement(2855..>3507)
                     /locus_tag="VE170_16840"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17161.1"
                     /translation="MARPEIKSLTEMRAKKIGITRLGSSTHSVTLWVMNKAGFKPEDY
                     QMLQLVDVPNIFTAIAAGQVDAGALSPPTNFRARKAGLSELMDVTKDGPEYVSVAIGS
                     TRSFIKANEEMTRRFMRGYSEGVQLLKANKAAGIRAIQKYARIKDPEILEATYGEARA
                     YIETIPYVTRKGMETIIGELLPTEAKAKTAKPEDFLDTRFVAQLEKEGFYKSGAAK"
BASE COUNT          844 a         1023 c          940 g          700 t
ORIGIN      
        1 cacatcgtcg cgccggattt gcgcggccac ggcgagtccg acaaaccgca aaccggttac
       61 cgcttgcggg atttcacaga agacatccgt cagttgatta agaatttaga attgaccaag
      121 cccgcctacg tcggccactc ctggggcggc aatatcggca cgatcctcgc cgccgactcg
      181 ccggcattaa tctcgcgagc gttccttgaa gatccggtct actggcgcat gattcacgcc
      241 ttcatgacga gcctgcccgg cgcgctggca cggcgcgcca aacccggagc cgagatccgc
      301 gctgaagcgc agcaaaaagg actgtcaaaa gcagacgaaa acctggaggt ctataaagcg
      361 catcacttct cgccccacgc cctgacccgc ctccagaccg ataaccgcga ctgggccatt
      421 gagtttgaga gttacttcaa gcgcatcaac gtgccgacga ccatcctggt cgccgaccac
      481 aaagccggcg gcgccatcat gcgcgaggaa ttgacctacc tccaaacact cgcaccgccg
      541 caggtaaaat tagaattctg ggagaacgtt ggccacggca tgaaggcggc caagccggcg
      601 gaatacaata gagcattgga aatttggctt ggttaatcac cagagagccg acctactccg
      661 atcgcgtccc gaggcgaaat ccgggcaaca aatagagcaa tcccgccagc ggtaccagcg
      721 gcggcaggtg cgggaatacc agcgtgttta ttagcggcaa agctccgacc aacaccagag
      781 tcaacgctac gcaaagcggc acttcgtccc cgccgatttt caagataaat gccgccggaa
      841 gagtaaacag ggtcatatca tggtcgtgaa agtggggtgc gatgagaatg atgccgatga
      901 gaatcttcac ccacgcagga aatgaatccg cttcaagtcc aagcgggcgg cggcactgaa
      961 tagcgaccac agtgatcaca ccagcggtca agcagtacca gaagtaatca cgccaggctg
     1021 gtgcggcgaa gaaataggcc aaagcacgca gattatgcat ccggtccggt cgtatgctca
     1081 gtgtggcttc gccggctgtg gcccgctgcg aaatgtccag atacgctcgt agtccttcta
     1141 agccgatcat aaagtaggaa attgcgccta gtccgaggat taccaacagc aagacgctga
     1201 ttccgcgcca ggattttttc gccaatagta gcagcgccgg tacaactaag agcggcggtt
     1261 tgaatagtaa aagcccacac caccaaccag cgcggcgctg gcgattatcg acaaaagctg
     1321 agacaaacag caccaacagg aggagtgcca aaaaagaggt ctgcgcctgt aacactgcat
     1381 aatacacacc atagttgcag acagtcgcga gcaccaacca ttttttctgc gcgaaattga
     1441 actcaagttg gcgcatgagc acactcaatg ccagcgccag cagcacaatg ttaaccaagg
     1501 tcatcaatgc gaacgcaacg gaaaaggaaa atcctgcgag cggcgccaac gccagtgcga
     1561 aaaaaggtgg ataaaaaaat ggcagcaccc agctgccgcg tgatggatcg atcggctgtt
     1621 gcacctggcg ctgccgttcc aaatcgtaga gcgccgacgg atcgctagtc accagcaccg
     1681 aaccagtata gagacttatg aaatcgggct tatatccact gcaactcgga ctctggtagc
     1741 ggtaaatcgt ccaccagcag ggaccgatta ccaaactcat ggcaattcct aagcccacag
     1801 ctagggctgc aatgcgattg gtccaggaat tttgaaaagc cattaggatg ggatgcgcgt
     1861 gccgagcgaa agagacggta ttttgatctc gaaccggtct cgccgttcag ccgtgcttct
     1921 taacccattc aagaatgact gcattagtct cctccggcgc ctgccagaaa aaaccatggg
     1981 actggccctt gagcattttc atttcggcac cgcgaatgcg ctccatcagt acttgagctt
     2041 gcgcccggtg attgctgcca accgtgtcgg cctcgccgat cagcaccaac gtcggtgcct
     2101 ggacatcgcc gagccggtgc gtgccttcga agttgtggcg cgcgatgcac agatgaatga
     2161 acggatcgag cttggcgtgg gtcgcccagg cgactttaaa aaacgtttcg acctgatcgc
     2221 ggtgttggtc gcgaaagtct ttcgtaaaaa acgtgtccga ctcgacgatc tcatcgtaaa
     2281 taaatttctc gaagcccatc tcgaccatgt cgtacaccat gcggtgcggc aaacccggca
     2341 cgcaatccga accggcccgg cccgccgtgc cggagccgct cgccgccatg atcagacttt
     2401 taactttgcc agggaaattt tgcgccagcg acaacgcgat gcgcccgccc atggagtggc
     2461 cgatgaagtg ggccgccggc aatttcaagt gatccatcat cgcgacgatg tccaaggcca
     2521 tctgctcgat ggtgtaaacc gattgcgtcg gcaccgagcg gccgcagccg cgcggatcgt
     2581 ggaagatcaa ttgcagagaa ctcgccagcg gcatctgcca cggcttccaa acctctccgg
     2641 aataagccgt cgacggcacg aatatcaacg gctcgccgtt gccgtgggtt tcgtaataga
     2701 aatcgacgcc ggtcggtaat tttacattgg gcatggatta tctctccttc gcagaaaggt
     2761 ttctcgctgc gctcgaaatg acaagacgtt agtcgctgtc atccgtgcat tgaatgtcat
     2821 ctcgaacgaa gtgagagatc tctccgagtc tcatttactt ggcagcaccg ctcttataaa
     2881 acccttcctt ctccaactgc gcgacaaacc gcgtgtcgag aaaatcttcc ggcttagccg
     2941 tcttggcctt ggcctcggtg ggcagcaact caccgatgat cgtttccatg cctttacgcg
     3001 tcacgtacgg aatcgtctcg atgtaagcac gcgcttcgcc gtaggtcgct tcgagaatct
     3061 ccggatcttt gatgcgcgcg tatttctgaa tcgcgcggat gccggcggct ttgttcgcct
     3121 tcaacagctg tacgccttcg ctatagccgc gcatgaaacg gcgcgtcatc tcttcgttgg
     3181 ccttaatgaa cgatcgggtc gaaccgatgg cgaccgacac atactccggg ccgtccttgg
     3241 tcacgtccat caattcgctc aagccggctt tacgggcgcg aaagttggtc ggcggcgaga
     3301 gcgcaccggc gtcgacttgg ccggccgcga tggcggtaaa aatattcggc acgtcgacca
     3361 attgcagcat ctgatagtct tcgggtttga accccgcttt gttcatcacc caaagcgtca
     3421 cactgtgagt cgacgagccc agcctggtaa tgccgatttt cttggcgcgc atttcagtta
     3481 agctcttgat ctccggccgt gccatga
//
LOCUS       DASQKS010000650         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5820056, whole genome shotgun sequence.
ACCESSION   DASQKS010000650 DASQKS010000000
VERSION     DASQKS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5820056"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1297
                     /locus_tag="VE170_16845"
     CDS             302..1297
                     /locus_tag="VE170_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17162.1"
                     /translation="MRIIFIWLLCCGWLGLTPVAFSAALEPVTIGYSTFAGAYLPLWI
                     AIEERLGSKYGLDLRAIYAGRIRPQQLLASNEVPFVMASGTGAITSHILGIKDQAIIL
                     VTINKVNSAIFSKPEIKRAEDLRGKTIATGRPGAFGDTMVRYVLRAKLGLVPDRDVKM
                     LPIGEAQLAFPALERGVVQAASLTLPFTLIAKKMGYRELIDYDQAGVVYPYNTVTALR
                     QTVVKHPDLTERVLKTLIEGSHIATTDREKSLAVMKKYLRGTSDDILDETYRYTKSVT
                     EEVPTPSLEITKAALEILSYEYPKAKDTDANLIIDTTIMKRIEQSGFIKSLYKNK"
     gene            1405..2361
                     /locus_tag="VE170_16850"
     CDS             1405..2361
                     /locus_tag="VE170_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17163.1"
                     /translation="MAAASTVSAAAKLETLTVGYSNFTGTYAPLWVAVEENLGAKYGL
                     DLKAIYAGRIRPQQLLAIGEVPIVLATATGTITSHILGVKDHVLVATITNKVGTSIFA
                     RGDIKSVEDLKGKTIATGRPGAFLDATVRYVLRSKFNLMPDRDVKLLPTGEPSLGLQA
                     LERGVVEAAAMSSPHMFVARKAGFRELASFDKLGVEYPYTSVVVLRQTVVKSPDLIER
                     FLKTIVEGIYVFKSNKTKTLAAFKRYMKGADDEILEATYQHSRATLEDAPHPTLQSVK
                     VALEMLALQYPQAKQTDPNLIVEPMFMKKIEDSGFIRALHKK"
     gene            2367..>3022
                     /locus_tag="VE170_16855"
     CDS             2367..>3022
                     /locus_tag="VE170_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ17164.1"
                     /translation="MADSDEILYDKQRQGVLITLNRPAAMNAATRPMLHKLGAALDDA
                     VADPQVRAIVLTGSGRGFSTGMDQGGGAAHRRDMDWPYGVRAGENAADFIDNYRGHEV
                     IFMRIFECSKPVIGAINGWAMGVGSWMSLFTHITIASEQAVFAQPEVRHGSNTGFIWT
                     LLAGFKDGLRYSLTGDHIDAQEALRIGLVNQVVPGDRLLDECFAIVERIAMVPPETVK
                     L"
BASE COUNT          669 a          873 c          889 g          591 t
ORIGIN      
        1 gctgttcggc agcgcgcagc ccagggactt gataccgccg acgcgaaaaa attatgacgg
       61 gcccttgatc gtcggtgagg acttgacgca aatagaaatc ggcagcgagg ttcgctccat
      121 accgttccaa cccaagctgg atcagtgact agggcagcgc agctcgttgt taggcgccgc
      181 aagccacctc tgggttgaaa tttatcgcga cggtgagcgc aggaaatgct ggatatcggc
      241 tcaaagctgt tgacgagcgg tgagtcgctg ggataaccca atctaaggaa ctatcttttc
      301 aatgagaata atttttatat ggctattgtg ctgcggctgg ctcgggctca cgccggtcgc
      361 tttttcggct gcactcgagc cggtcaccat cggttactcg acgtttgccg gcgcctatct
      421 gccactctgg attgccatcg aagaacgatt gggcagcaaa tacggtctcg acctgcgcgc
      481 gatctatgcc ggccgcatca gaccgcaaca gctcctggcg agtaacgaag tgccgtttgt
      541 catggcttca ggcaccggcg cgatcacttc acatattctc ggtatcaagg accaagcgat
      601 tatcttggtc acgatcaaca aggttaattc ggcgatattc agcaagccgg agatcaaacg
      661 ggccgaagat ctacgcggta aaaccattgc caccggccgc cccggcgcct tcggcgatac
      721 catggtgcgc tacgttttga gggccaagct tgggctcgtg ccggaccgcg atgtgaagat
      781 gttgccgatc ggcgaagcgc agctggcttt cccggcgctc gagcgcggcg tggtccaggc
      841 ggcgtcgttg acccttcctt ttactttgat cgccaaaaaa atgggctatc gggagttgat
      901 cgattacgac caggcagggg tcgtatatcc gtacaatacc gtcaccgcgc tgcgccagac
      961 ggtggtgaag catcccgatc tcaccgagcg ggtcttaaag acgctcatcg aaggcagtca
     1021 tatcgccacg accgatcggg aaaaaagttt ggcggtgatg aaaaaatacc tgcgcggaac
     1081 cagcgacgat atcctggacg aaacctatcg ctacaccaag agcgtcaccg aagaggtgcc
     1141 gacgccgtcg ctggaaatca ccaaggcagc gctggaaatt ctctcctatg aataccccaa
     1201 agcgaaagac accgacgcca acctgatcat cgatacaacg atcatgaaac gcatcgaaca
     1261 gagcggcttt atcaaatcct tgtataaaaa taaatgacga gaggatgcac aatggccggg
     1321 tcacggtgta gacggcggtg tcgggtcaaa ccaagaaacc gacttcgctg ggtcgcaacc
     1381 gccatcgcac tgttgccgct atggatggcg gccgcctcga cggtctcggc ggcggccaag
     1441 ttagaaaccc tcaccgtcgg ttattccaat tttaccggca cctatgcgcc gctctgggtg
     1501 gccgtcgaag agaatcttgg cgctaagtat ggattggatt tgaaagcgat ctacgccggg
     1561 cggatccggc cgcagcagtt gctcgctatc ggtgaagtgc cgatcgtgct cgccaccgcc
     1621 accgggacga ttacctcgca tattctcggc gtgaaagatc atgtgttggt ggcgacgatc
     1681 accaacaaag tcggcacctc gatattcgcc cgcggcgata tcaaaagcgt cgaagatctc
     1741 aaaggcaaga ccatcgccac cggccggccc ggagcgttct tggacgcgac cgtgcgctac
     1801 gtgctgcgga gcaaattcaa tctgatgccg gaccgcgatg taaaactatt gcccaccggc
     1861 gagccgtccc tcggtttgca ggccctggag cggggcgttg tcgaagcggc ggcgatgtcg
     1921 agtccgcata tgtttgtcgc gcgcaaagcc ggcttcaggg agttggccag cttcgacaag
     1981 ttgggcgtcg aatatcctta caccagcgtc gtggtgctgc gtcaaacggt ggtgaagagc
     2041 ccggacttga tcgagcgctt cctaaagacc atcgtcgagg gaatttatgt cttcaagagc
     2101 aataagacga agaccttggc ggcgttcaag cgctacatga aaggcgccga cgatgagatc
     2161 ctcgaagcaa cctaccagca tagccgcgcg accttggaag atgcgcccca tccgaccctc
     2221 cagtcggtga aggtggcgtt ggaaatgttg gcgctgcaat acccgcaagc gaagcagacc
     2281 gacccgaatc tcatcgtcga gccgatgttt atgaaaaaga tcgaagacag cggtttcatc
     2341 cgcgcgctgc acaagaaata aacgccatgg ccgacagcga cgagattctc tatgacaagc
     2401 aacgtcaggg cgtgctgatc acgctcaatc gtccggcggc gatgaacgcg gcgacccggc
     2461 cgatgttgca caagctcggc gcggcgctcg acgatgccgt cgccgatccg caagtgcgcg
     2521 ccattgtcct caccggctcg ggacggggct tttccaccgg catggaccag ggcggcggcg
     2581 cggcgcaccg gcgcgacatg gactggccct acggcgtgcg cgccggcgag aacgcggcgg
     2641 actttatcga caactaccgc ggccatgagg tgatcttcat gcgcatcttc gaatgctcca
     2701 agccggtcat tggtgcgatc aacggttggg ccatgggcgt aggctcatgg atgtcgttgt
     2761 tcacccatat caccatcgcc tcggagcagg cggtgttcgc ccagccggaa gtgcgccacg
     2821 gctccaatac tggattcatc tggaccttgc tcgccggctt caaagacggc ctgcgttact
     2881 cccttaccgg cgaccatatc gacgcccagg aggcgttgcg catcggcctg gtcaaccaag
     2941 tcgtgcccgg cgaccggctg ctcgacgagt gttttgcgat tgtcgaacgg atcgccatgg
     3001 tgccgccgga aacggtcaag ct
//
LOCUS       DASQKS010000651         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5412566, whole genome shotgun sequence.
ACCESSION   DASQKS010000651 DASQKS010000000
VERSION     DASQKS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5412566"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..643
                     /locus_tag="VE170_16860"
     CDS             239..643
                     /locus_tag="VE170_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HYJ17165.1"
                     /translation="MASFVEAGSGVQIHLEVTGLSPGLHAVHIHAVGKCEAPAFTSAG
                     GHFNPSQKKHGHRSQEGAHAGDLPNMLVAKDGSGRFEVFTDGVTLRAGPLSVFDADGS
                     ALVVHAGVDDYVTDPTGNAGDRAACGVIVARN"
     gene            complement(680..1672)
                     /locus_tag="VE170_16865"
     CDS             complement(680..1672)
                     /locus_tag="VE170_16865"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HYJ17166.1"
                     /translation="MRRRDFLKYTALGSLSTTLANCQSSGRWQPAAFRKKSSSRVAVL
                     TAKGYDQPLKDVLLRGIKLCGLQVKGKRIVVKPNLVEFDPKGVINTNPTLIEAAIDAF
                     RTLGAAEVIVAEGPGHRRDNEYLLSASGLYAVLREHKVRYVDLNSDDVRATPVRSSFT
                     NFKQLYLPETLFNADLLVSMPKLKTHHWAGVTLSMKNLFGIIPGTVYGWPKNILHWQG
                     IDNSILDINSSLPLPQFAIVDGIVGMEGNGPLQGQAKNAGVLLFGDDFVSVDASAARL
                     MKLEPRKVVHLAKADAFLGNLASERIEQIGEKLESLQQDFDIIEDLKDLKTLTG"
     gene            complement(1682..2584)
                     /locus_tag="VE170_16870"
     CDS             complement(1682..2584)
                     /locus_tag="VE170_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HYJ17167.1"
                     /translation="MLDQIITELLQNQNPDGGWGAVRGKRSNTETTSLAVMALQALAG
                     GTSADNRRRGIEWLVRRQNPDHSWPLNQTVTAGSWTTALAITALGASVEHSERVHAAA
                     RWVLEQKGSKPGILAEIILWATGQSGVNKLSKDLIGWSWVPSSFSWVEPTSYALIALK
                     KQRLHLTGTNVDERIREADAMIYDRMCTGGGWNYGNSKVLDYALWPYPDITALALIAM
                     QDHASEKANQDSLQVLSKTARETDSGLALCWAATCFNLYGRDPSDFHKQIEKRFATSE
                     FLGETKTLALAVIALGGKSNPFRF"
BASE COUNT          550 a          747 c          822 g          636 t
ORIGIN      
        1 tcggcgcaat tttgcgaata agatagccgg tagtcgtaac acattggttg cgagccgggt
       61 ccatcgatgc taacgatttg ccatgcaaaa ttttttatat tcaaaattac gtgattcccg
      121 ggtagttacc attgtggttt gcgccttgtt gttggcagcg ggagcaggct gctcattagt
      181 cggctccggc aaatctacga cgtccgaact caaggacaag gatgggaaga gcgtcggaat
      241 ggcgtcgttc gttgaagcgg gcagcggcgt gcagattcat ttggaagtga ccggactgag
      301 cccgggcctg cacgcggttc atattcatgc tgtcggtaaa tgcgaggccc ccgcatttac
      361 cagcgccggc ggacatttca acccgtctca aaagaaacat ggccatcgca gtcaggaggg
      421 tgcccacgcc ggcgatttgc ccaatatgct ggtggcaaag gacggctcag gccgtttcga
      481 agtgttcacc gacggagtga ccctccgcgc cggacccctg tcggtctttg acgccgatgg
      541 cagcgccctg gtggttcacg ccggagtcga cgattacgtc accgacccga cggggaatgc
      601 cggtgatcgc gcggcctgcg gtgtgatcgt cgcgcgtaac tgatgcttcg cgtttggcga
      661 tcgaatcaga ggttcggctt cagccggtta gggttttcaa atcttttaga tcctcgatga
      721 tgtcgaagtc ttgctgcaga ctctccaact tctcgccgat ctgttcgatt ctttcgctag
      781 ccaagttgcc gagaaaggca tcggccttgg ctagatgcac gactttgcgc ggttcgagtt
      841 tcatcaggcg cgccgcgctg gcgtcgacgg acacaaagtc gtcgccgaac agcaacaccc
      901 ccgcgttttt cgcttggcct tgcagcgggc cgttgccctc catgccgacg atgccgtcga
      961 cgatggcgaa ctgcggcagc ggcagggagc tattgatatc caagatgctg ttgtcgatgc
     1021 cttgccagtg cagaatattc ttcggccagc cgtagaccgt ccccggaatg atgccgaaca
     1081 ggtttttcat cgacagcgtg acgccggccc aatgatgggt tttcaacttt ggcatggaca
     1141 ccaataggtc ggcattgaac aaagtttctg gcaggtacaa ttgcttgaag ttggtaaaac
     1201 tgctgcgcac cggagtggcg cggacatcgt cggagttgag atcgacgtag cggactttat
     1261 gttcgcgtag gacagcataa agtcccgagg ccgagagcaa atattcgttg tcgcgccgat
     1321 ggcccggccc ttcggcgacg atcacctcgg cagcgccgag agtgcgaaag gcatcgatgg
     1381 ccgcttcgat gagagtcggg ttggtgttaa taacaccttt gggatcgaat tcgaccaggt
     1441 ttggtttgac aacgattctt tttcctttga cctgtaatcc acaaagcttg atacctctca
     1501 gtagcacgtc tttgagcggc tggtcgtacc ccttagccgt caagacggcg acgcgcgagg
     1561 aggatttctt gcgaaacgcc gcaggctgcc agcgcccgga agactggcaa ttggcgagtg
     1621 tcgtgctgag cgagcccagg gcggtatatt taagaaaatc tcggcgacgc atgatttaat
     1681 cctagaagcg aaatggattg gacttaccgc ctagcgcaat cactgccagc gccaaagttt
     1741 tggtttcgcc gagaaactcc gaggtggcga agcgtttttc gatctgcttg tgaaagtcag
     1801 atgggtctcg accatagaga ttgaaacaag tggctgccca acaaagcgcc aaaccggaat
     1861 cggtctcgcg cgcagtcttg cttagcactt gtaggctgtc ctgattggct ttttcactcg
     1921 catgatcttg catggcgatc agcgccagcg cggtgatgtc cgggtagggc caaagcgcgt
     1981 aatcgagaac cttggagttg ccataattcc acccgccgcc ggtgcacatg cgatcgtaga
     2041 tcatcgcgtc ggcttcgcga atgcgctcgt cgacattggt gccggtcaga tggaggcgct
     2101 gttttttcag cgcgatcaaa gcatagctgg tcggttccac ccagctaaat gagctcggta
     2161 cccaggacca gccgatgaga tctttgctga gtttattgac gcccgattgg ccggtggccc
     2221 agagtatgat ttcggccagg atgccgggct tgctgccctt ttgttcgagc acccagcgcg
     2281 ccgcggcgtg cacacgttcg ctatgctcga cactagcgcc gagggcggtg atggctagag
     2341 ccgtcgtcca agaccccgct gtgacggttt ggttgagcgg ccagctatgg tctggatttt
     2401 ggcgccgcac caaccactcg ataccgcgcc tgcggttatc cgctgaggtc ccgcccgcta
     2461 gcgcttgcag cgccatgacg gccaatgagg ttgtttcggt gttgctgcgc ttaccgcgca
     2521 ccgcgcccca gccgccgtcc ggattttgat tttgcagcag ttcggtgatg atttggtcga
     2581 gcatgactaa ttggccgtgg tacgtcaggt gagaacttat tatagcaggt ctttagccgc
     2641 agaactgggg ccggctatcg gagaaaatgc agccgcgtct ggccgcagcc gagctgacgt
     2701 tccgcgtccg caatccgttc tttggtaccg agatcttgcc agaagccgtc aaagc
//
LOCUS       DASQKS010000652         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4533459, whole genome shotgun sequence.
ACCESSION   DASQKS010000652 DASQKS010000000
VERSION     DASQKS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4533459"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VE170_16875"
     CDS             complement(<1..679)
                     /locus_tag="VE170_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HYJ17168.1"
                     /translation="MTDSELILLRALLAELAPQGRPALLPALHAAQQIHGWIPEPVAA
                     EVGRALGVPLADVFGVIDFYQMFSSQPVGRTVARVCGAPVCALAGADALADALCRHLQ
                     IEPGEVSSDGAFTVAHAPCLGLCDHAPAVLVGETAVGHADAEQAAAICALNAHKTESL
                     VGGDIRILTSNCGQGHPISLSDYQARGGYAGLKKALSMTPQAVLAEVKASGLVGRGGA
                     AFPTGVKW"
     gene            complement(746..1531)
                     /locus_tag="VE170_16880"
     CDS             complement(746..1531)
                     /locus_tag="VE170_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17169.1"
                     /translation="MKTPQAGERFSEQEAKEYVDHLYKTLYKYAHEKVYKGAFMTQLR
                     DGKLPLPAMRQFFKNWGHFSLEVNALNAVSYYTHLPFFVRHFDLLGPFCAKIADELIS
                     PKAPGHVLVLLQTAEKMGLNKKEMLEDPYLPAARAINDFCHKIFIDGSIVELWGLHVF
                     EESLSMWSGQWFTALTTHYGFSKKDAIYFSTHEEADMDTHKLGDSDEEAMGHGAFNRT
                     VLTRALQESVEFRAGYTLEYCATTMVDLHAQMKQAALDYPYHP"
     gene            1654..2010
                     /gene="queF"
                     /locus_tag="VE170_16885"
     CDS             1654..2010
                     /gene="queF"
                     /locus_tag="VE170_16885"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HYJ17170.1"
                     /translation="MNSEASKSLETFANPRPERDFEINMECPEFTCVCPRTGQPDFAT
                     IRIAYVPDKICIELKSLKLYLWSYRNEGTFHEAVTNRILDDLVAACQPRRMTVVGDFN
                     VRGGIHTTVTASYTQS"
     gene            2299..3678
                     /gene="dnaB"
                     /locus_tag="VE170_16890"
     CDS             2299..3678
                     /gene="dnaB"
                     /locus_tag="VE170_16890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HYJ17171.1"
                     /translation="MAALEDNLRKIPPQNLEAESSVLGGILLENDAINVVLETLRPED
                     FYRESHRRIMRAMIEITDRNEPVDLITLSEFLKTKNELESVGGSAYLASLADFVPTAA
                     NIAFYVRIVHEKAILRNLISTATEIATRGYDEQGNVDEFLDTAEKVIFDISERKIKQS
                     FVAVGDMITETLKTIDKLYQRKEMITGVSTGYEDLDKITAGLQPADLIIVAGRPGMGK
                     TAFALNIATNAAYTGVGAAVFSLEMGKEQLVLRMLCSEARVNSSKVRSGYLGERDFPQ
                     LAKAAGRLHEAPIYIDDTPAISVLELRAKARRLVRDRSKKVGLIVVDYLQLMRGMGNA
                     NNREQEISEISRSLKALAKELSVPVIALSQLNRRLEDRSERRPQMSDLRESGAIEQDA
                     DVIMFIYRDEVYNKNNDDLKGKAEVIIAKQRNGPTGSIDLTFIGEFTRFENYTERDDY
                     GGRDEIGEL"
     gene            3769..>3949
                     /locus_tag="VE170_16895"
     CDS             3769..>3949
                     /locus_tag="VE170_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HYJ17172.1"
                     /translation="MLFKLWLLLAVCTFAASFTGAGSVNAAQAPALLLANELGPQIDP
                     AKYLISEKYDGVRALW"
BASE COUNT          882 a         1106 c         1114 g          847 t
ORIGIN      
        1 cccatttgac gccggtggga aaggccgcgc cgccgcggcc aaccaaaccc gatgctttaa
       61 cttcggcgag cacagcttgg ggtgtcatcg acaaagcttt tttaagtccg gcataaccgc
      121 cgcgcgcctg gtagtcggac agcgagatgg gatggccttg gccgcaattg ctggtcagaa
      181 tgcgaatgtc gccgccgacc aagctctctg ttttgtgcgc gtttagcgcg cagatcgctg
      241 cagcttgctc ggcgtcggca tgaccgacag cggtttcgcc gacaagcact gcgggcgcat
      301 ggtcgcacaa tcccaagcag ggggcatgcg cgacggtgaa tgcgccatcc gagctgactt
      361 caccgggctc gatctgcaag tggcggcaca gcgcgtccgc cagcgcatcg gcgccggcaa
      421 gggcgcacac cggcgcgccg cagacccgcg ccacggtccg gcccaccggt tggctggaga
      481 acatctggta aaaatcgatg acaccaaaga catcggcgag cggcacaccc aaagcgcggc
      541 ccacttcggc agcgaccggt tcgggaatcc agccatggat ctgctgagcc gcatgcaagg
      601 cgggcaggag cgccgggcgg ccttggggcg ccaactcggc aaggagcgcg cgcaggagaa
      661 tgagttcgct atcggtcatt acgacacgac gacatttttt ctttgcgatc tcggcgcgag
      721 caagctcccg tgcggctcgg ctgcactagg gatggtaagg ataatctaac gcggcctgtt
      781 tcatctgcgc gtgcagatcg accatggtcg tggcgcaata ttccaacgtg tagccggcgc
      841 ggaattcgac gctctcctgc aaggcgcggg tgagcacggt gcggttgaaa gcgccatggc
      901 ccatcgcttc ctcgtcgcta tcgcccaact tgtgcgtgtc catgtcggct tcttcatggg
      961 tggagaaata aatcgcgtct ttcttgctaa agccgtagtg cgtggtcagt gcggtgaacc
     1021 attgtcccga ccacatcgac aatgactctt cgaaaacatg caggccccag agttcgacga
     1081 tggagccgtc gataaaaatc ttgtggcaaa agtcgttgat ggcgcgggcc gccggcaagt
     1141 agggatcttc gagcatttct tttttgttca agcccatctt ctcggcggtt tgcaacaaga
     1201 ccaaaacgtg acccggcgct ttcggcgaga tcaattcgtc ggcgatcttg gcgcagaacg
     1261 gccctaacaa atcgaagtgg cgcacgaaga agggcagatg ggtgtaatat gagaccgcat
     1321 tgagcgcatt tacttccaac gaaaaatggc cccagttctt gaaaaactgg cgcatcgccg
     1381 gcagcggcaa cttgccatcg cgaagctggg tcatgaaagc gcctttatag accttttcat
     1441 gggcatactt gtagagggtt ttatacaaat gatcgacgta ctctttggct tcttgctcgc
     1501 taaatctttc gcccgcttgg ggagttttca tggtggcagc tccaatctcc gacaggattc
     1561 aaccaagaca tcccggtgtt atcattcgag tttttgagag tcaaggtaag tgggcgtttt
     1621 gcaatggtat tgaggtgtga ttaagatgat tccatgaatt ccgaagcaag caaaagttta
     1681 gaaacctttg ccaatccgcg gccggagcgc gattttgaaa tcaatatgga gtgtcccgag
     1741 tttacttgcg tctgccccag gaccggccag cctgacttcg ccaccatccg cattgcctac
     1801 gtgccggata agatctgcat cgaactcaaa tcgctcaagc tctatctgtg gtcataccgc
     1861 aatgaaggca cgtttcacga ggccgtgacc aacaggatcc tcgacgattt ggtcgccgcc
     1921 tgtcagccgc gcaggatgac ggtggtcggc gacttcaatg tgcgcggcgg cattcatacc
     1981 acggtgaccg cgagctatac gcaatcctag cctcgagttg cccaatccgt cggcgcctag
     2041 catcgccgca tcgataccgg agagcagttt gggggtaagt tgtttataag ctgtgcgtga
     2101 gtttttcgaa gtggccgagt aaattcgata atcgaattgt ttgaccggtt tgcctaaaag
     2161 ctgcgcgcca accgtgccga aaataattct ccaagcccgg aaaaaattat ttctgatttt
     2221 agagttttta ctatcagaaa agaaataatt tctgctatgg tgatcgccct gatctgtggt
     2281 tcgaagtgaa aaccatccat ggctgccttg gaagataacc tgcgcaagat tccgccgcaa
     2341 aatttggagg ctgagtcttc tgtcctgggc ggcatcttgt tggaaaacga tgcgatcaac
     2401 gtggtcctgg agaccttgcg ccccgaagac ttttaccggg agtcgcaccg ccggatcatg
     2461 cgcgccatga tcgagatcac cgaccgcaac gagccggtgg atctaatcac cctgagtgaa
     2521 tttcttaaaa ccaagaacga actggaatct gtgggcggca gtgcttactt agcgtcgctt
     2581 gccgattttg tgccgacggc ggccaacatc gccttctacg tacggatcgt ccacgagaag
     2641 gcgattctgc gcaatctaat ttcgacggcg accgagatcg ccacccgcgg ctatgacgag
     2701 cagggcaatg tcgatgagtt cctcgacact gccgaaaaag tcatcttcga tatttctgaa
     2761 aggaagatca agcaatcctt cgtcgccgtc ggcgatatga ttaccgagac gctcaagacg
     2821 atcgataagc tttaccaacg caaggaaatg atcaccggcg tgtccaccgg ttacgaggac
     2881 ttggacaaga ttaccgccgg attgcagcct gcggatttga tcattgtggc ggggcgcccc
     2941 ggtatgggca aaaccgcttt cgcccttaat atcgccacca acgccgccta caccggcgtc
     3001 ggtgccgcgg tgttttcgct ggaaatgggc aaggagcaat tggtcttgcg catgctttgt
     3061 tccgaagcgc gggtcaatag ttccaaagtt cgctccggct acctgggcga gcgcgacttt
     3121 ccccagctcg ccaaagccgc tggcaggctg cacgaagcgc cgatctacat cgacgacaca
     3181 ccggcgatat cggtgttgga actgcgcgcc aaagcgcgcc gtttggtacg cgaccggtcg
     3241 aaaaaagtcg gcctcatcgt cgtcgattat ttgcagctca tgcgcggtat gggaaacgcc
     3301 aacaaccgtg agcaggagat ttcagaaatt tcccggtcgc tgaaagcgct ggccaaagaa
     3361 ctatccgtgc cggtgattgc gctatcgcag ctcaatcggc ggctagaaga ccgctccgag
     3421 cgacgtccgc agatgtccga cttgcgcgag tcgggagcca tcgagcagga cgccgacgtg
     3481 atcatgttca tctaccgcga tgaggtctac aacaagaaca acgacgattt gaaaggcaaa
     3541 gccgaagtga tcatcgccaa acagcgtaat ggccccaccg gctccatcga tctgacattc
     3601 atcggcgaat tcacccgctt cgagaactat accgaacgcg acgactacgg cggccgcgac
     3661 gagatcggcg agttgtaagc gcgcagcgct tacccatgat aattgcaaaa taccgattcc
     3721 actttatcca tcccgccact tcgccaggct cgtctataga tgcgccccat gttgtttaag
     3781 ctttggttgt tattggcggt ctgcacgttc gctgcatcgt tcaccggcgc cggctccgtc
     3841 aacgcggccc aggcaccggc gttgctcctc gccaacgagc ttgggccaca gatcgatccc
     3901 gcgaaatatc ttatcagtga aaagtacgac ggcgtgcgcg cgctgtggg
//
LOCUS       DASQKS010000653         5208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3320459, whole genome shotgun sequence.
ACCESSION   DASQKS010000653 DASQKS010000000
VERSION     DASQKS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320459"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VE170_16900"
     CDS             <1..1167
                     /locus_tag="VE170_16900"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ17173.1"
                     /translation="IHHLVHGLEFYAGLASKVRGSHVPLPQKNAYGMVVRQPIGVCGG
                     IVPWNFPLTLMGTKLGPALAAGNTIIIKPASTTPLATIKVIELIAQATFGEGKAQKQL
                     PKGTVNIVTGPGGSVGEELLRNPRVRRIAFTGSTPIGRHVMEVAGREIKRVTLELGGS
                     DPMIVMEDSNVDLAVKMADVGRYFNCGQMCLGVKRLYLQESIADNFIGALVEILKKKT
                     VGNGMSKETRMGPIHVGAQRQEVEEQVEDAKSRGGKVLFGGERPKGADFDNGFYYLPT
                     LMTHVPEDSRLTKEEVFGPALPVYTFKTLDEALEKANASEFGLGSSIWTRNLLHANKA
                     IDKLQAGNVWVNSLHYGYDELPFGGVKSSGVGREHGPEALDYYLEPKGVVVVNV"
     gene            1232..2029
                     /locus_tag="VE170_16905"
     CDS             1232..2029
                     /locus_tag="VE170_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ17174.1"
                     /translation="MFAQMRHMAIVTSNANHEGDFYRDIFGMKRSGLARAGGAVVVGD
                     GYIGLNLNPRAPGRQAGFDHFGFEVQDTEVVFARLKDKYPKVQVLHRPSNRPFAGISM
                     HDPNGNVFDLSQANMENRRDIYTDQVEERKRRIGHFGLRCVDPDLCGEFYRDVFELQE
                     LEKPNGDPNHYLSDGKVTLAVMPWKIADFAGSGIERPALDHIGFKVESLAAFKADVEK
                     HAHLYAVEENRPEKNPERDVRQKLLASCCFGQYQMSDPDGVLIDVTE"
     gene            2235..2588
                     /locus_tag="VE170_16910"
     CDS             2235..2588
                     /locus_tag="VE170_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17175.1"
                     /translation="MKRRAWITFLLAAFVSAAIWLLTPMLTNKREPWDVEGSFYLVAL
                     TIAGAVAGAIAPGPLWAHYAGAVAGQLGYELIVVRVGPLILLGALFLLVYCAIFTLAA
                     ALAAFVRQRVAERFG"
     gene            2638..3309
                     /locus_tag="VE170_16915"
     CDS             2638..3309
                     /locus_tag="VE170_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-tRNA-protein transferase"
                     /protein_id="HYJ17176.1"
                     /translation="MSASNLDEHFLCLRASPAKMDLYWAEGWRHFGVIFVRYRMSSHG
                     GKPYTVLPLRVDLARFQLTRSQKRVLAKNRDIEVIVRPSFINNEKQILFDRHCVRFDD
                     NVPDSLFNFLSPNPAEIPCPNLELCIYLHGRLVGVTFLDIGELATSAIYAIFEPAEAK
                     RSLGILMILHSIAFSRERGCRYYYPGYAYREPFTYDYKKRLTGLEYLDWDVGWQPYTE
                     AGFTR"
     gene            complement(3383..4417)
                     /locus_tag="VE170_16920"
     CDS             complement(3383..4417)
                     /locus_tag="VE170_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17177.1"
                     /translation="MGSLLKSSLAIVVMGLLSVASQSAAQTPYYAGKTITIIRGGGAG
                     GSGEFQSRALVPYLKKYIPGNPNVVMEFMDGASGRKAANYFYTVKPDGLKIASAGNMI
                     SGPILGLSGSNYDIEKFPYIGSTETGNPYVFFSRKVGNLDTLDKIRNARGLRVGAHSV
                     GHSVYITGRMFAHMLDLKEPRFVVGFNDPEMDIALANSELDGRTSGNIDSVLKDLRDQ
                     IYVHATISNPKGRFDPRLPGLPDLDSFAKNDRERKVIDLFRAFQVPRWPHHLAPGTPA
                     ELVKILRDAMARTFKDPEFQQDFKKLMGREPSPLTGEEVQKVVKELPRDAETVALYKI
                     YAEGGALPPR"
     gene            4649..5104
                     /locus_tag="VE170_16925"
     CDS             4649..5104
                     /locus_tag="VE170_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ17178.1"
                     /translation="MSDNAEKKARALGINHVVLEVDDLDQALEFYGALFEFTLRGKSE
                     HNAFIDLGDQFIQLTLGRSQSADTKRHFGFVVDDRDAVRRSMEKLGIKSINDRLNILD
                     PWGNRIEIVPYDDCQFTKAAHILKGMAVEPPAKTANAIEELKKKGMSAQ"
BASE COUNT         1130 a         1526 c         1433 g         1119 t
ORIGIN      
        1 attcatcatc tcgttcatgg cttggaattt tacgccggcc tggcgtccaa agtccgcggc
       61 tctcatgtgc cgctgccgca aaaaaatgcc tacggcatgg tcgtgcgcca accgatcggc
      121 gtgtgcggcg gcatcgtgcc gtggaatttc ccgctgacgt tgatgggcac aaagttaggc
      181 ccggcgctgg ccgccggcaa cacgatcatc atcaagccgg ccagcaccac gccgttggcg
      241 acgattaaag tcatcgaact gatcgcccag gcgaccttcg gcgaaggcaa agctcaaaaa
      301 cagttgccca aaggcacggt gaatattgtc accggcccgg gcggcagcgt cggcgaagaa
      361 ctgctgcgca atccgcgcgt gcgccgcatc gcgttcaccg gttcgacgcc gatcggccgc
      421 catgtcatgg aagtggccgg tcgcgaaatc aagcgagtca ctttggaatt gggcggcagc
      481 gacccgatga tcgtcatgga ggacagcaat gtcgatttgg ccgtgaagat ggccgatgtc
      541 ggccgttatt ttaactgcgg ccagatgtgc ctgggcgtga agcgcctata cctgcaggaa
      601 tctatcgccg acaatttcat cggcgccctg gtggaaatct tgaaaaagaa gaccgtcggc
      661 aacggcatga gcaaagaaac ccgcatgggg ccgattcatg tcggcgccca gcgccaagaa
      721 gtcgaagagc aagtcgaaga cgccaaatcc cgtggcggta aggttctctt cggcggtgag
      781 cgtcccaagg gtgccgactt cgacaacggc ttttactatc tgccgaccct tatgacccat
      841 gtgccggaag actcgcgctt aaccaaagaa gaggtcttcg gtccggcgct gccagtctac
      901 accttcaaaa ctctcgacga agctttggag aaagccaacg catccgaatt cggcctcggc
      961 tcatcgatct ggacgcgcaa tcttttgcac gccaacaaag ccatcgataa gctccaagcc
     1021 ggcaatgtct gggtgaactc gctgcattac ggttatgacg aattgccctt cggcggggta
     1081 aaatccagcg gcgtcggccg cgagcatggc cctgaagcgc tcgactatta cctcgagccc
     1141 aaaggcgtgg tcgtcgtcaa cgtctgagcg ccggttgaca agccgcctac gcttcaacta
     1201 cgttagctta actaacccaa aggagaccgc tatgtttgca caaatgagac acatggctat
     1261 tgtcaccagc aatgccaatc atgaaggtga cttctatcgt gatatttttg gcatgaagcg
     1321 gtcgggactg gcgcgagccg gcggcgccgt ggttgtcggc gacggttata tcggcttgaa
     1381 tctcaatccc cgcgcccctg gacgtcaggc gggcttcgat catttcggct tcgaggtgca
     1441 agacactgag gtcgtttttg cgcggctcaa agacaagtat cctaaagtcc aagtgctcca
     1501 tcggccgagc aaccgtcctt tcgccggcat cagtatgcac gatcccaacg gcaacgtctt
     1561 cgatctatcg caggcgaata tggaaaaccg ccgcgacatt tacaccgacc aagtcgaaga
     1621 gcgcaaacgg cgcattggtc acttcggttt gcgctgtgtc gacccggatc tgtgcggcga
     1681 attttatcgc gacgtgtttg agctgcagga gttggaaaaa cccaatggcg accccaatca
     1741 ctatctttcc gacggcaaag tcaccctggc tgtgatgccc tggaagatcg ccgacttcgc
     1801 cggttccggc atcgaacggc cggcgcttga tcacatcggc ttcaaggtcg aaagtcttgc
     1861 cgcgttcaaa gccgacgtcg agaaacatgc ccatctctac gccgttgaag aaaaccgtcc
     1921 ggaaaaaaat cccgagcgcg acgtgcggca gaagctgctg gcatcctgtt gcttcggcca
     1981 atatcaaatg tccgatcccg acggcgtgct gatcgacgtg acggaatagt agcaagtgga
     2041 tttgttcgag gtaaagggcg accgttgctt gacggccgcc ctttttattt ggcggggtta
     2101 tttcgctctc gcaattcagc taaaccttgt catggcttgg tatcgttatc gccgtcagtc
     2161 gtagcgcgcc ccataccttt ggcaagcaag ccgtaaccca gcactcgatc gcgtagaaat
     2221 agagaaagcg caagatgaag cgacgtgcct ggattacatt tttgcttgcc gcctttgtga
     2281 gcgcggcgat ttggctgctg acgccgatgc tcacgaacaa acgtgagccc tgggatgttg
     2341 aaggcagttt ttatctcgtg gcgcttacca ttgccggggc agttgccggc gccatcgctc
     2401 caggaccgct atgggcgcac tatgccggcg ccgtcgcggg ccagcttggc tatgaactga
     2461 tcgttgtccg cgtcggtcct ctcatcttgc tcggcgcgtt gttcctgctg gtctactgcg
     2521 cgatctttac cctcgcggcg gcgctggcgg cttttgttcg tcaacgggtt gctgaaagat
     2581 ttggttaatc gagttgaccg cgccgtggca aatgattcaa gattgcgtta acgatccatg
     2641 agcgcatcga acctagacga acatttcctg tgcttgcggg cctcgccggc caagatggac
     2701 ctttactggg ccgagggctg gcgtcatttc ggcgtgatct tcgtgcgcta ccgcatgtca
     2761 tcccatggcg gcaagccgta tactgtcctg ccgttgcgcg tcgatctcgc gcgttttcag
     2821 ttgacccgca gccagaagcg cgtgctggcc aagaatcgcg acattgaagt gatcgtccgg
     2881 ccgtcattca tcaataacga aaaacaaata ctttttgacc ggcattgcgt ccgcttcgat
     2941 gacaatgttc ccgactcact gtttaacttt ctctcgccca acccggcgga aattccctgc
     3001 cccaatctgg agctgtgcat ttatctccac ggccggctgg tcggcgtcac gttcctcgac
     3061 atcggcgaac tcgccacgtc ggcgatctac gcgattttcg agcccgccga ggccaagcgc
     3121 agcctgggca tcctgatgat tttgcattcc atcgccttta gccgcgaacg cggctgccgt
     3181 tactactatc ccggttacgc ctaccgcgaa cccttcactt acgattataa gaagcgcctc
     3241 accggtctgg aatatttgga ttgggatgta ggatggcaac cttacaccga agcaggcttc
     3301 acgcgctaat tttagacagg cggatttaac tggaagaact ccagtcccac ccgccgttaa
     3361 ctggcaaata cgccagcaaa actcagcgcg gcggcaacgc gccgccctcg gcatatattt
     3421 tgtaaagcgc cacggtctcg gcatcgcgcg gcaactcctt caccaccttc tgcacctctt
     3481 ctccggtcag cggcgacggc tcgcgcccca tcagtttctt gaagtcctgc tggaattccg
     3541 gatctttgaa agtccgtgcc atcgcgtcgc gcaggatctt caccagttcc gccggcgtac
     3601 ccggcgccag atgatgcggc caacgcggca cctgaaaggc gcggaacaag tcgatgacct
     3661 tgcgctcgcg gtcgttcttg gcaaagctgt cgagatcggg tagccccggc agtcgcgggt
     3721 cgaagcggcc cttgggattg gagatcgtcg catgaacata aatttggtcg cggagatcct
     3781 tgagtacgct atcgatattc cccgacgtgc gtccgtccag ttcgctgttg gccagcgcga
     3841 tgtccatctc cggatcgttg aagccgacca cgaaccgcgg ttctttcaag tccaacatat
     3901 gcgcgaacat gcgtcctgtg atgtaaaccg agtggcccac cgagtgagcg ccgacgcgca
     3961 atccgcgcgc attacgaatc ttgtcgaggg tatcgaggtt gccgactttg cggctgaaga
     4021 acacgtaggg attgccggtt tccgtcgagc cgatataagg aaacttttcg atatcgtagt
     4081 tgctgcccga caggcccagt atcggtcccg aaatcatgtt tcccgccgac gcgatcttca
     4141 agccatcagg tttcacggta taaaaataat ttgccgcctt gcgccccgac gcgccgtcca
     4201 taaactccat gacgacattg gggttgcctg ggatatattt cttcaagtag ggcaccaagg
     4261 cgcgcgactg aaactccccc gaaccgccgg cgccgccgcc gcgtatgatg gtgattgtct
     4321 tgccggcata atagggcgtc tgagcagcgc tctgacttgc caccgacaac agtcccatca
     4381 ccacaatagc caacgatgac tttaacaatg atcccatcct cattcctccg atttttaaac
     4441 ctcttaactc caactagagc gatcagtccg ctgttcttga gatgcgttgg cttatccata
     4501 aggcagatgc cgaggcagcg tcaagggtca atcgtcgctg accatgcgtg gcggcttgga
     4561 cgcggttcat gatatgactt caccggagcg caattttgca tgcggaagac agtgccggcg
     4621 gaaaaattaa atcaggagga gactggtcat gagcgataac gctgaaaaga aagcccgcgc
     4681 cctgggtatc aaccatgtag tcctggaagt cgacgacttg gatcaagcgc tggaattcta
     4741 cggcgccctg ttcgaattca ctctgcgcgg caagagcgag cacaacgcct tcatcgatct
     4801 cggcgaccaa ttcatccagc tcactctcgg ccgcagccaa agcgccgata ccaagcgcca
     4861 cttcggcttc gtcgtcgacg atcgtgacgc ggtgcggcga tcgatggaaa aactaggcat
     4921 caaaagcatc aacgaccggc tcaacatcct cgatccctgg ggcaatcgca tcgaaatcgt
     4981 tccctacgac gattgccaat tcaccaaggc agctcacatc cttaaaggca tggcggtcga
     5041 accgccggcg aaaacagcca acgccatcga agaacttaag aaaaaaggca tgtcggcgca
     5101 gtgaaatgga tgcggcaacg cctgaccagg ctccgagttc agggtcggaa atatccacac
     5161 catgggttcg atggcgcatc gccgatggcg gtttcggcgg ctgctgcg
//
LOCUS       DASQKS010000654         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2904934, whole genome shotgun sequence.
ACCESSION   DASQKS010000654 DASQKS010000000
VERSION     DASQKS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2904934"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VE170_16930"
     CDS             complement(<1..227)
                     /locus_tag="VE170_16930"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYJ17179.1"
                     /translation="MLKEKVGVVGAGKIGAAIMRGIVRAGLAGKDQVMASDVSAHLRD
                     DVVKELDIKATADNGALCDFADVIILAVKPQ"
     gene            complement(352..3792)
                     /locus_tag="VE170_16935"
     CDS             complement(352..3792)
                     /locus_tag="VE170_16935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYJ17180.1"
                     /translation="MAVGVPRFRQDWLLLVGLFCFFFSGAAGLIYQVVWTRMLSQIFG
                     NTTYAIATVLSAFMAGLAIGSYWFGKIADRGKNDFLLYGVLEFGVGVYGFLVPWFFAG
                     AQKVYGPIFSLNESQPFLFNLFLFFLSFVLLVFPTLLMGATLPVLSRFFVRSFAQFGR
                     RVGDLYATNTLGAVIGCALGGFYLIPVYGMRATVYVAAGVNMLIAIVILIVDRLRDPE
                     PVAAEAEAAARSRDAASAAAQSSTYLDWVLLGSFALSGFASLVYENAWTRSLTLVIGS
                     SIYSFTTMLVTFLVGLALGGFIYARVLGNREARLSTFGLVEIWVGLSALATIPLFERL
                     PLIFVRLLHGFGDTFTVFLYLQIFLSALVMFIPTILLGMTFPLVARLFTQSLYRVGSG
                     VGSSYAANTVGAVLGAFAGGFILIPHLGVQNTIICGVVLNLVIGCWLIVSDSRLSFLP
                     RYALGIAVLILAAIIPFKVKRWDPSILTSGVTIYNDRYEALPSDSLRIEEMKRDEVLF
                     YREGLTTTVSVHKILGSDYIYFKSNGKIDGSYGDALSQLMTSYIAMMLHPKAETALTI
                     GLGSGMSAKALATFKSLKDIEVIEIEPAMIEASKFFDRQSVRVDKFPAGVTFPADDSS
                     GRVWFNAAEKRLYYKGVMSDAARTELMNRSDDKDYRGMIDRLYRNARHSRHSSVLEDK
                     RVRVIPTDGRNYILATPKFYDVITAEPSNPWIAGIANLYTREFYQVIKSKIKDDGIFA
                     QWFHNYSMSPDDFRMVFRTFAEAFPHVSLWSMKESDFLLIGSKQEQKFDYPAVKAIFD
                     SNEMLRSDFQYLGLSDVYAVQGFYRMGRDGFLAFSKGAKINTDDGAELEFSAPKNLRR
                     ATTELNRRIMTPHLADGGPPWLKKGSLPVPQAMHHFYLAQSYEASVARNRALTELEEA
                     IRLEPTNPKFQLLKMKILLEQDKSTEGAQAAFKALEHGTEYIPEVLAMSDEFYLPDAK
                     LVYKKVIDMGSKEVLPYLGLGNIALHSGDIAEAEKWFIPARELQPEHPAVLLAWGRLT
                     AAKGNRTADAAQAKKERQEARRLLELSKTKGEESATIFTELGAVYSKLEMWDKAAENY
                     EVALRMRRRRNDLRMELGKSYAKLGRTVEAEQKFRQVLAFSPDDADAWHELQLMGKAY
                     "
     gene            4110..>4368
                     /locus_tag="VE170_16940"
     CDS             4110..>4368
                     /locus_tag="VE170_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17181.1"
                     /translation="MAQVKRDGASPEEARDLRSAIEWMKKEGDIIVTDKEVDPNLELT
                     GIQKKLDGGCPILFNNVKGKPQHRCVTNLFGDMNVINKMFGW"
BASE COUNT         1060 a         1235 c         1189 g          884 t
ORIGIN      
        1 atctgcggtt tgacggcgag aatgataaca tcggcgaaat cgcacagcgc gccgttatcc
       61 gcagtcgcct tgatatcgag ctccttgacc acgtcgtcgc gcagatgcgc gctgacatcg
      121 ctcgccatca cttgatcttt accggccaaa ccagcgcgca cgatcccacg cataatcgcc
      181 gcaccgattt tcccggcgcc gaccacgccg actttttcct tcaacattgg cgcctcctca
      241 ttcaaaaact atgggcgacg acagcgcagc ccaacgagtc gatcttgcgt tctgctccga
      301 atgtcgtgct tgtgggccgt tgttcacatg ataaaccgcg ctattcggaa ctcagtaggc
      361 cttgcccatc aactgcaact cgtgccaagc gtcggcgtcg tcagggctaa acgccaacac
      421 ctggcgaaat ttttgctcag cctccaccgt ccggccgagc ttggcgtagg attttcccag
      481 ctccatgcgc aggtcgttgc ggcgccggcg catgcgcagg gcgacttcat aattctccgc
      541 cgccttgtcc cacatctcaa gcttagagta gaccgcccct aattccgtga atatggtggc
      601 ggattcctcg cctttggttt tcgatagctc gagcaaccgg cgcgcttctt gacgctcttt
      661 cttggcctgc gccgcgtcgg cagtgcgatt gcctttggcc gcggttaacc ggccccaggc
      721 tagtaacact gccggatgtt ccggctgcaa ctcacgggcg ggaatgaacc acttttccgc
      781 ttcggcgata tcgcctgagt gcaacgcgat gttgccgaga cccaggtacg gcaacacttc
      841 tttacttccc atgtcgatga cttttttgta aaccagcttg gcgtcgggca aataaaactc
      901 atcgctcatg gcgagcacct cgggaatata ctcggtgccg tgctccagcg ccttgaacgc
      961 cgcctgcgca ccctcggtac tcttatcctg ctccagcagg attttcattt tcagcaattg
     1021 aaacttggga ttcgtcggct ccaagcggat cgcttcttcg agctcggtta gggcacggtt
     1081 gcgcgccacg ctggcttcgt aggattgtgc caagtaaaaa tgatgcatcg cctggggcac
     1141 cggcaaggaa ccttttttca gccaaggcgg accgccatct gcaagatgcg gcgtcataat
     1201 ccgccggttg agttcggtgg tcgcgcgccg caggtttttg ggcgcggaaa actccaactc
     1261 cgcaccgtca tcggtattaa ttttcgctcc cttggagaac gccagaaagc cgtccctgcc
     1321 catgcggtaa aagccctgga cggcataaac atcggacagg cctagatact ggaagtccga
     1381 acgcagcatt tcgttgctgt cgaaaatcgc tttgaccgcg ggatagtcaa atttctgctc
     1441 ctgtttgctg ccgatgagca agaagtcgct ttccttcatg ctccacagcg agacatgggg
     1501 aaacgcttcg gcgaaggtgc gaaataccat gcggaaatcg tccggcgaca tggaatagtt
     1561 gtgaaaccac tgggcgaaaa tcccgtcgtc tttgatcttc gacttgatga cctggtaaaa
     1621 ctcgcgggta tacaggttgg cgataccggc gatccacgga ttggatggtt cggcagtaat
     1681 gacatcgtaa aatttcggcg tcgctaatat atagttgcgc ccatcggtgg ggatcacccg
     1741 cacccgtttg tcttccaaaa cgctcgagtg gcgcgagtgg cgggcattgc ggtacagccg
     1801 atcgatcatg ccacggtagt ctttatcatc cgagcggttc atcaactcgg tgcgtgcggc
     1861 atcgctcatg acccctttat aatagagacg cttttccgcc gcgttgaacc agacgcggcc
     1921 actgctatcg tcggcgggga aggttactcc cgctgggaat ttgtcgacgc gcaccgactg
     1981 gcggtcgaaa aacttactcg cttcgatcat cgccggctcg atctcgatga cttcgatatc
     2041 cttgagagac ttgaatgtcg ccagcgcctt ggccgacatg ccgctgccca gcccgatggt
     2101 cagcgcggtt tcggccttcg gatgcagcat catggcgatg tagctggtca tcaactggct
     2161 cagcgcatcg ccgtaggaac cgtcgatttt accgttggat ttgaagtaaa tataatcgct
     2221 gccgagaatc ttgtggacgc tcaccgtggt ggtcagtccc tcgcggtaaa atagcacctc
     2281 gtcgcgcttc atctcttcga tacgcaatga atcgctcggc aaggcctcgt agcggtcgtt
     2341 ataaatcgtc acgccgctgg tcagtatgct ggggtcccaa cgcttgacct tgaagggtat
     2401 aatcgccgcg agaatcaata ccgcaatgcc gagcgcataa cgcggcagga acgacagccg
     2461 cgaatcgctg acgatcaacc agcaaccgat caccaagttc agcacgacgc cgcagatgat
     2521 cgtgttctgc acgccgaggt gggggatcaa aatgaagccg ccggcgaagg cgcccagcac
     2581 cgcgccgacg gtatttgccg cataggagct gccgacgccg ctgccaacgc gataaaggct
     2641 ttgggtaaat agccgcgcca ccagcggaaa cgtcattccg agtagaatag tcggaatgaa
     2701 catcaccagt gccgacagaa aaatctgcaa gtagaggaaa accgtgaaag tgtcgccgaa
     2761 accatgcagc aagcggacaa agatcagcgg caggcgctcg aatagcggga tcgttgccag
     2821 cgctgacagg ccgacccaga tctcgaccag accgaaggtg ctcaagcgcg cttcgcggtt
     2881 gcccaggacc cgcgcataaa taaatccgcc caacgccagg ccgacgagaa acgtgactag
     2941 catggtggta aacgaataga tcgagctgcc gatgaccagg gtgagcgagc gcgtccaagc
     3001 gttttcgtaa accaatgagg cgaaaccgga gagagcgaat gagccgagca atacccagtc
     3061 gagataggtt gagctttgcg ccgcggcgct ggcggcgtcc cggctgcgcg cagcggcctc
     3121 cgcctcagcg gccaccggct caggatcgcg caggcgatcg acaatcaaaa taacgatggc
     3181 gatgagcata ttgacgccgg cggcaacgta aaccgtagcg cgcatgccat agacgggtat
     3241 taagtaaaat ccgcccaggg cacagccaat caccgcgccc agcgtgttgg tggcgtaaag
     3301 atcgccgacg cggcggccga attgcgcgaa gctgcgcacg aaaaagcggc tcaacaccgg
     3361 cagagtcgcc cccattaata gagttggaaa caccaacaag acgaaggaaa gaaagaacag
     3421 gaatagattg aaaagaaacg gctggctctc attgagagaa aagatcgggc cataaacctt
     3481 ttgcgccccg gcgaaaaacc atggcacgag aaatccgtag accccgaccc cgaattcgag
     3541 cacgccgtac aacaagaaat cgtttttgcc gcggtcggcg atcttgccga accagtagct
     3601 gccgatggcc aatccagcca tgaaggcgga caacaccgtg gcgatggcgt aggtcgtatt
     3661 gccgaatatc tgcgacagca tccgggtcca cacgacttgg taaatcaatc cggcagcgcc
     3721 ggaaaaaaag aaacaaaaca gaccgacgag taaaagccag tcctgtctaa agcgcggaac
     3781 gccaacggcc ataaattctc ctcaaaaaca taatccagca cagatagcag gccgcatagt
     3841 aactgtaaag tcgcataata cagacaaaat ttcccatgcg ccggtggcgg cttggggcga
     3901 ttgcaggggc gccgttcgac ccggttgggc gaccgagggt tcgttgactt tgagataaat
     3961 tctgataatc gtaagcacca ttcaaacagc ctgattcgga taatttccca taaattttat
     4021 tgccgattag gtctaaaaaa gctgcggatt tagagaattt tttgaccacg ttcagttaaa
     4081 aaaacagtta aatcattcgg aggaaaagaa tggcacaggt taagcgtgac ggcgcatcgc
     4141 cggaggaagc aagggatcta cgcagtgcga tcgagtggat gaaaaaagaa ggagacatca
     4201 ttgtcaccga caaagaagtc gaccctaacc tagaactcac cggcattcag aaaaagctcg
     4261 atggcggctg cccgatcctc tttaataacg tcaaaggcaa gccgcagcat cgctgtgtca
     4321 ccaatttatt cggcgacatg aacgtcatca acaagatgtt cggctgga
//
LOCUS       DASQKS010000655        13802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1629058, whole genome shotgun sequence.
ACCESSION   DASQKS010000655 DASQKS010000000
VERSION     DASQKS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629058"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VE170_16945"
     CDS             <1..441
                     /locus_tag="VE170_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17182.1"
                     /translation="TGVALLTMSLGIAACSTQDEMVAKPAAKPAAPAPAATAKTEAKA
                     EAKGDAKAPAKDEAKSEAKAAATKLAGDVGLLDTEKNYLIVVTKEGKLVTVDYDAKFQ
                     PTQRELKEAKVADIGLGSAATVLYTKDGDKNIASSIEFLPAKGE"
     gene            492..914
                     /locus_tag="VE170_16950"
     CDS             492..914
                     /locus_tag="VE170_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17183.1"
                     /translation="MKTPKQGIGALAIIFAASLALPVVAQEKKAEQEKKAEKVEKAEK
                     SEKGEGAKAEPAKAKAEKIAGDIGLLDTEKNYMIVVTKDGKLVTLDFNNKTKATEIVE
                     TKVKMGDVGLGSSANVEYVTKGEKNFLSKMTFVPAKGE"
     gene            1315..2049
                     /locus_tag="VE170_16955"
     CDS             1315..2049
                     /locus_tag="VE170_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYJ17184.1"
                     /translation="MADLSKLRELVAQSCRMLGKLNLTKEPSGHVSARIPGEDKVLIK
                     ARGPEESGLRFVTARDIITVDFNGKKIAGGDGLDVPQEVFIHTWLYKSRREVESVVHV
                     HPLTVVLFTICDKPIQPLYGAYDPSGLRLIVEGLASYPRSITVSNEKLGEEFAKAMGD
                     KPACLMRGHGITSAGPGVEDATLTAIKLNDAATINYQAHLLGSPRPIPQEDIDILVGK
                     SRGAGNPSVHANSNWRYYCKLLDEES"
     gene            complement(2543..3448)
                     /locus_tag="VE170_16960"
     CDS             complement(2543..3448)
                     /locus_tag="VE170_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17185.1"
                     /translation="MMRSVKSLVKRWKTAGTGVAVLLILRALVPAPVWPQELPETQLE
                     EEIAKQGKIFQGRGAEVPSGYVTNRGLSDYADLLPTGFCDALGRLGNSDRWLDIGAGA
                     AQAILDYYAPAGNTAPAEKCALSVSKARAVAMSIEDRRTDKWRKQAANVGNDRIRYLA
                     GKSLRHYSLEELGKFQIITDVFGGFTYTADLSRFVDKVLNLLEVRGEFYTLAPGVHLE
                     GGDKLGTWYLTELENAAGRPEKVCSWLKKATCVKVTCESKNDWKRPTELIKIEKVCSD
                     VSVPRMKLLEFVAGYPPDRRFQLEP"
     gene            3909..4298
                     /locus_tag="VE170_16965"
     CDS             3909..4298
                     /locus_tag="VE170_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17186.1"
                     /translation="MTDKIIPEEVKQFIFEKIDSVAEMEALLLLRSNPEASWSIASLA
                     KRLYIDEGQTAKIVDHLCREGLLTISSNEQASYRYEPKSVELQNSVDRVAEMYTKHLV
                     PISTLIHSKPRVRAQEFADAFRLRKDK"
     gene            4301..4558
                     /locus_tag="VE170_16970"
     CDS             4301..4558
                     /locus_tag="VE170_16970"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HYJ17187.1"
                     /translation="MAEAFYALCTVTALFCSLLLLRGYFRTRYKLLLWGGLCFLGLTL
                     NNALLVADKLLFPEVNLFTWRLLSALLAMLVLLYGLIWDAD"
     gene            4575..4859
                     /locus_tag="VE170_16975"
     CDS             4575..4859
                     /locus_tag="VE170_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HYJ17188.1"
                     /translation="MLLGAISMASLVAGLFFLRFWRDTRDRFFLFFAVSFIIEGINRA
                     ALALTSNPNEGLPFFYFVRFLSFLLIFVAILHKNQKKDTRVKPSKGDGLR"
     gene            complement(5055..5567)
                     /locus_tag="VE170_16980"
     CDS             complement(5055..5567)
                     /locus_tag="VE170_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ17189.1"
                     /translation="MGSVALSVVREALQPVLLFRPQAHREAPNKIITVAAALDGSPFS
                     EEILPHAVKAACSLSARLLLLQALPVIRPPSPLSREERADIVESSYLYRRAAEIQDAH
                     GIEAQWEALHGEPGDALCSYLKDMPETLLAMTTHARDEAKRVVMGSVAGNCVRHAGVP
                     LLLYWPPKRN"
     gene            complement(5946..6383)
                     /locus_tag="VE170_16985"
     CDS             complement(5946..6383)
                     /locus_tag="VE170_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005369012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HYJ17190.1"
                     /translation="MNIPFQITFRNMAPSAAIEKHIREKAVKLESFCDRITNCRVTVE
                     VPHRHHHKGNAFQVRIDLSAPGCEIVINRAPKRLSAAKLTDSRQVEKNFLENHRPSKH
                     AAHEDVYVAIRDAFNAAGRKLQDHMSRQSGATKVHEEIARAEE"
     gene            6673..7158
                     /locus_tag="VE170_16990"
     CDS             6673..7158
                     /locus_tag="VE170_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYJ17191.1"
                     /translation="MAIIKKLKELFDEAKVSYEVYNHPLAYTAQEIAQRQHFSGDQMA
                     KVVILKVDGKLVMVVITGNQKVHLPTVRASLGADEVRLATEDEFISKFPDCEIGAMPP
                     FGNLFGVIVYLDPAVAKDELIYFNAGNHVQTVRIRYKDFARLVNPEVVRLTEDRTKHA
                     A"
     gene            complement(7323..7592)
                     /locus_tag="VE170_16995"
     CDS             complement(7323..7592)
                     /locus_tag="VE170_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17192.1"
                     /translation="MQHKKEMTFQQNMSLFWALVRAGAVASAKTLSSDRLRAISSFET
                     NEFYMEDYEADCRALANCYQSYSLAKYFGPTYDHGIGDRVEKACA"
     gene            8185..8580
                     /locus_tag="VE170_17000"
     CDS             8185..8580
                     /locus_tag="VE170_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17193.1"
                     /translation="MLVLQASSCANFGAWVRRYTYPPTFRYITDEQLRSTMWQLAYHS
                     RELNDLMAREAFASQRAEVMQHLRLMEQAMLDLNRTGWPTNHPLVDAKRSNFLRDILN
                     AQEAVSRDSPSFLLAGAVSGACAYCHPNR"
     gene            9479..12130
                     /locus_tag="VE170_17005"
     CDS             9479..12130
                     /locus_tag="VE170_17005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ17194.1"
                     /translation="MSDVNPNLQTDISAKQQSFEESELLLNLYRALIENRDLGSGLRA
                     AVKIVCEFTRWIVGTAWLPSKDETKIGLCSFWHQDDPKLAEFIVSCQHQQFARNVGIL
                     GRVWHRQKYEWTRNLAVEPTDSFPLAPAAANAGLKAAFALPIMHNHDVDAVLCFHARE
                     AREEDGRLIEVISRIANQLGFALQHKGLEEELLQQQALVLRTHGNLEEEVERRTAELR
                     LANQMLQAEVSGRELNERRMQERTRQQEALANLGRQALGGTDLSALMHTITELVATSL
                     NVEFCKVLELLPGSDTLILRAGVGWKVGLVGRATVSAGTNSQAGYTLLCKEPVIVKDL
                     PTEERFSGPPLLCDHGVISGLSVIIGQSDRPFGVLGAHTARARVFSQDDVHFLQAIAN
                     VFSQVIEHNLAIEELHRNATWLKRLIETTQDAVVSIDRRGCIVLFNAAAERVFGYRAD
                     EVVGRKVNDLMAEPYATEHDGYIARYEETGEPRAIGRIRSVEGRRKSGETFPLELSVT
                     QVATGESEKVHYAAFIRDISEVRRGQAWLQSLIEATQDAVLSIDRRGRIALFNPAAER
                     IFGYTRDEVIGQKVNMLMAEPYATEHDEYIARYEKTGEARAIGRIRSVTARRKDGEPF
                     PIELSVTEIAMDQEVHYAAFIRDISEKTKLQGQLVESERLAAIGSTAAKIGHELANPL
                     NGMSLTVQLLEQRITRLNLDAISQLTPTVKRLRDEISRLQKLAGEFATIARKEKYVFR
                     PVNLMQLVDDVVKLQALHFARNGVEIQISLAEELPSISIDSDKMKQALLNLIKNSAEA
                     MPRGGRIIFNAFVADGAVILEITDTGDGIPLDIDAFEPFLTTKREGTGVGLVIVRQIL
                     TAHNGKISYRSKPGEGTTFRIELPLTL"
     gene            12271..13608
                     /locus_tag="VE170_17010"
     CDS             12271..13608
                     /locus_tag="VE170_17010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ17195.1"
                     /translation="MIGTILVVEDQDSARESLVDLLKNENFEVHAAADGKAGIELVDQ
                     HDLDLVLTDLAMPGADGIAVLRHIRDVSPQTLVILMTAHATVDTAVEAIRLGAQDYLL
                     KPLIFEDVLRKVQHLMNHRKLAWENQLLRREINTRFSPERPLGRSQAMQDITHLIKKV
                     APTPTTVLITGESGVGKEVVARTIHTASRFNENVFLPVNCSAIPENLLESQLFGHIKG
                     SFTGAVNSQEGLFQRARGGTIFLDEIGEMPLSLQPKLLRVLEEKAVLPVGSTNPVKVN
                     VRILAATNRDLKTDVEAGRFREDLFYRLNTFEINIPPLRKRLDDLPGLIEHLVQRHNV
                     EMNTSYKGVDSATMRILMSFPWKGNIRELDNVLERAMILGDGDWIKPADLPGHQADAG
                     EFNSEDNLSAALDLYEKSHIERTLNKAAGDKMRAAELLGLSLSTLYRKLEKLA"
BASE COUNT         3318 a         3489 c         3739 g         3256 t
ORIGIN      
        1 actggagtgg cattgttaac gatgtcgctg ggtattgcag catgttcgac gcaagatgag
       61 atggttgcca agccggcggc gaaaccggcg gcaccggccc ccgcggcaac ggcaaagaca
      121 gaagctaaag ctgaggcaaa aggagacgct aaggctccgg caaaggatga ggcaaaaagc
      181 gaagccaagg ccgccgcaac caagctggcg ggagacgttg ggctactgga caccgaaaag
      241 aattacctga ttgtcgtgac caaggaaggc aaactcgtaa cggttgatta cgatgctaag
      301 tttcaaccga cccagcgcga gctgaaggag gccaaggtgg ccgatatcgg tctcggctcg
      361 gccgccacgg tcctttacac aaaggacggc gacaagaaca tcgcttcgag catcgaattt
      421 ttgccggcga aaggcgagta ggcgcctagg aaccggtcga gaaaagacgc atcagtaagg
      481 agaattcaaa aatgaaaaca ccaaagcaag gaatcggcgc attggcaata atcttcgcgg
      541 catcgctagc gttgcctgtg gttgcgcaag aaaagaaggc tgagcaagaa aagaaggctg
      601 agaaggtgga aaaggccgaa aagtctgaga agggggaagg cgccaaagcc gaacccgcca
      661 aggctaaggc ggaaaagatc gccggtgata tcggtctgtt ggacaccgag aaaaactata
      721 tgatcgtcgt caccaaggac ggcaagctgg tcactttgga ttttaacaac aagaccaagg
      781 cgacggaaat cgttgaaacc aaagtaaaaa tgggcgacgt cgggcttggc tcctcagcca
      841 acgtcgagta cgtcaccaaa ggtgagaaga atttcctgtc gaagatgacc ttcgttcctg
      901 ccaagggcga atagttccgg cctatcaagc ttgaagacct aagggggcgg ttacaacctc
      961 ggttgtaccg cccttttttt ctgtctgagt ctgcgagcca attcctccag ccgggttgcc
     1021 gaagaacgct agatcgtaag ccgcaaataa aagttcataa aacacataca agctgtgcac
     1081 ggggtgggat tggaaagcgc ctctagggtg cgaatctctc actcatttca cctcgccatt
     1141 ttgtgtattc gtgttgtttc gatgtcggct atccacggta tggaagccac ccatattcga
     1201 tcagggcagg caccctccaa ggttatctgt tcgcttgcct tagtcgggac gcgagaatat
     1261 gatccggtcg ctttacaata gtccattgcg aaattcttga atggagaaat ttttatggcc
     1321 gatctatcga aactccgcga gctcgtggcc caatcctgcc ggatgctcgg caaactcaac
     1381 ttaaccaagg agccaagcgg ccatgtcagc gcgcggattc ccggcgaaga caaagtgttg
     1441 atcaaagcgc gtggcccgga agaatcgggg ctgcgcttcg tcaccgcgcg cgacatcatt
     1501 accgtcgatt tcaacggcaa aaagatcgcc ggcggcgacg ggctcgatgt gccccaggaa
     1561 gtgttcatcc acacctggct gtacaagagc cgccgggaag tcgaaagcgt ggtacacgtc
     1621 catcccttga ctgtcgtgtt atttacgatt tgcgacaaac cgatccagcc cttgtacggc
     1681 gcctacgatc cctcgggttt gcgcttgatc gtcgaagggc tggcgagcta cccgcgcagc
     1741 attacggttt ccaatgagaa actcggtgaa gagttcgcca aggcgatggg tgataagccg
     1801 gcatgcctga tgcgcggtca cggtattacc agcgccggtc ctggcgttga agacgcaacg
     1861 ctgacggcga tcaagctcaa cgacgccgcg accatcaact atcaagccca cctactcggt
     1921 tcaccgcggc cgatacccca ggaagacatc gacattttgg tcggtaagtc acgcggcgca
     1981 ggcaacccat cggtgcatgc caactccaac tggcgctact actgcaaact gctcgacgaa
     2041 gagtcgtaga ctggggcgtt caagccgttc aaatcgtttt gaatgtcgga tgccgccgat
     2101 attcattttg catccataat gccgtcgtcc ttgagtttcg cgatgtggca aaccaattcg
     2161 tgcgatcaat gccgtcgtgg atgagtctgc tgtcagtcgt gcagctggtt ccggcgattt
     2221 tttgttggtg tcgttggctg ataaatcgcc gcatcgacat gccaggcggc gggcttatcg
     2281 tggcgatctg ctggatcgct tacggtgtac cggattcccc ctggtgctcg aaatagcgtg
     2341 agtgggcagg tgtgttaaga tgctatagtt gcttcggccg acttaataaa ccccgccatt
     2401 tatttctttc caataggaag cagctcttta gtttggatac gtctgcgtag tcatgaaatg
     2461 tgccgcgccg ttggggaagt ctgaaaccca tcgacctgga cgcgtcattg atcgttgtcg
     2521 ttacccaact gtgatcatca ccctaaggct ccaattggaa acgtctgtcg ggaggatatc
     2581 ccgcaacgaa ctccaataat ttcatgcgag ggacagagac atcgctgcag accttctcga
     2641 tttttattag ctcggtgggc cttttccaat cattcttcga ttcgcaggta actttcacgc
     2701 aagtggcctt cttcagccaa gagcacactt tctctggacg gccggcggca ttctcgagtt
     2761 cggtcagata ccaagtgccg agcttatctc caccctctaa gtgcacgccg ggcgccagcg
     2821 tgtagaattc gccgcggacc tccagtaagt tcaacacctt gtccacgaac cgggacaagt
     2881 cagcggtata agtgaagcca ccgaacacgt cggtaattat ctggaacttt ccaagttcct
     2941 cgagtgaata atgccgcagg gactttccgg cgagataccg tatacggtca ttgccaacgt
     3001 tcgcggcctg ctttcgccac ttgtcggtcc ggcggtcctc tatggacata gcgaccgcgc
     3061 gtgctttgct caccgatagg gcgcactttt cagccggtgc cgtgttgcct gcgggtgcgt
     3121 aatagtccag tatcgcctgc gcggcgcccg cgcctatatc caaccatcgg tccgagttgc
     3181 ccagcctgcc gagcgcatca caaaagccgg tgggaagaag atccgcgtag tctgagagcc
     3241 cccgattggt gacgtagccg ctaggaactt cggcaccgcg cccctggaag atttttccct
     3301 gtttggcgat ttcctcttcg agctgggttt cagggagttc ctgtggccag acaggtgctg
     3361 gaacaagtgc tcgaaggatc agcaaaacgg ctacgcctgt tcccgcggtc ttccatctct
     3421 ttaccaacga cttgactgaa cgcatcataa ttatttcccc cgccgttgaa aacgtgccgt
     3481 acttgaaaca tcttcaagct gttccagcta tagtctatgc tgccggtagg ccgcaagccg
     3541 ttcgcgatcc acaggccgcg tttgggccag cccgccgaaa agactcatat ccttcttgcg
     3601 gtcgatcgtc tcggtataac gtactcgcta agtacttgat cagctgaatc caggtttttc
     3661 ataaaagatg ctaggccgtc ttggcaaaac cttcgttcac gtctttctcg cagtgaagcg
     3721 gggttatgtt tgcaatctcg ggctacgccg gcggcaagaa caagcagtac aataagcctt
     3781 cactatttgt tttatggtgc agaggcatct ctcgacaaga agcaaacgca ttgatacctg
     3841 tgcaagaagg gaaccggaga tttcacggtt ttccagttcg ggagatgaca tggcagttgg
     3901 gatgaactgt gaccgacaaa ataatccctg aggaagtaaa gcaatttata tttgaaaaaa
     3961 tcgactctgt cgcagaaatg gaggcactcc tccttctgcg cagtaaccca gaagcctcgt
     4021 ggtccattgc cagtttagca aaaagacttt acatcgacga aggacagacc gccaagattg
     4081 tcgatcatct ttgcagagaa ggattgctga cgattagctc aaacgaacag gcaagctacc
     4141 gctatgagcc caagtctgtg gagcttcaaa atagtgtgga tcgagtcgcc gaaatgtaca
     4201 caaaacatct tgtgccgata agtaccttga tccattcgaa gccaagggtg agagcccagg
     4261 agttcgctga cgcgttcagg cttagaaagg acaagtaact atggcggagg ccttctatgc
     4321 cctttgcacg gtgaccgcgc ttttctgttc attgttgcta ctgcgaggct atttccgtac
     4381 tcgctacaaa ctattgttat ggggagggct ctgcttcttg gggttaacgc ttaataacgc
     4441 attgttggtt gctgacaagt tgttgttccc ggaggtgaat ctctttactt ggcggttact
     4501 cagcgcgctc ctcgcgatgt tggttttatt gtacgggtta atctgggacg ctgattaagt
     4561 tatttatgga cttgatgctg ctaggagcaa tctcgatggc ctcattagtt gcggggctgt
     4621 tttttctacg cttttggcgg gatacgcgag ataggttttt tctttttttt gccgtttcgt
     4681 ttatcatcga agggattaat cgggcggcac tcgcattgac gagcaatcca aacgaaggac
     4741 tgccattttt ttattttgtg aggtttctct cttttcttct catctttgtt gccattcttc
     4801 ataagaatca gaagaaggac acaagagtga aaccctccaa aggtgacggt ttacgctaac
     4861 gtatctatgg gccgagttct ccgtgaaacg gcgcggtgag atataatagg ggtcaccaaa
     4921 gtcgttatga gcaattgttt tagaaatagg cggtgatgca aactatttgc gcggtgcatg
     4981 ataagaatgg cgggtgcgct gaaagcctca tagtgttggg cgaaaaattc aagcagcatt
     5041 acccgaccga ttgatcaatt ccgctttggc ggccaataga gcaagagagg cacgcccgca
     5101 tgacgaacgc aatttccagc cacgctaccc atgacgactc gcttcgcttc gtcgcgggcg
     5161 tgggtcgtca ttgcgagtaa cgtttccggc atatctttga gataactaca aagtgcatcg
     5221 ccaggttcgc cgtgcagcgc ttcccattgc gcttcgattc catgagcgtc ttggatttcg
     5281 gcggctctgc ggtacagata tgacgattcc acgatgtctg cacgttcttc gcgtgacaag
     5341 ggcgatggcg gtcggattac cggcagcgcc tggagcagca gaaggcgcgc ggatagcgag
     5401 caagcggcct tgacagcgtg cggtaatatt tcctcggaaa acgggctgcc gtcgagagcg
     5461 gcggccacag tgataatctt gttcggcgcc tctcgatgtg cctgcggtct aaacaagagc
     5521 acgggttgca aagcttcgcg cacgacgctt aaggcgacgc ttcccatgat cacttcggtc
     5581 caagcagttc gcccgtgtgt agtcagcgcc gcaatggcgc gcggttctct atggagttcc
     5641 gcccggatgg cggtggaagg atctggggat acggcaaatc gcaactccgc tccatattgg
     5701 cgggcgcagt cgcgcaaata gtcctcttca ctcgacattt catcgggact ctcgacgacg
     5761 cgcagaaaaa cagctttcct ttcgtcactt ggatgatgcc acgcgctaaa ggaaggatct
     5821 tttcagagag agatgaaccg tccaagcaaa ctatgatttc atcgaacatg ataacctcgg
     5881 ttttgccgaa ggcatctcgc ggcatcggaa tgggagcgcc gcagcggtcg ttgattcgat
     5941 aacttttatt cctccgcgcg cgcaatctcc tcatgcactt tagtcgcgcc gctttgccgg
     6001 ctcatgtgat cctgtagctt tcgcccagcg gcattgaacg cgtcacgtat ggcgacatag
     6061 acgtcctcat gagcggcgtg cttgctgggg cgatgattct ccaggaaatt cttttcgact
     6121 tgccggctgt cggttaactt ggctgcactc aaccgtttcg gtgcgcggtt aattacgatt
     6181 tcgcagcctg gcgcactgag gtcaatgcgc acctgaaatg cattgccctt gtggtggtgt
     6241 cggtgcggca cttccacggt gactcggcag ttggtgatcc tgtcgcaaaa cgactccagt
     6301 ttcactgcct tttcgcggat atgtttttcg atggcggccg acggcgccat gttgcgaaag
     6361 gtaatctgga agggaatgtt catgcctatg gtcctcccgt tgttatttct aatacggaag
     6421 ccggcgagag ccgtgccata ttcattttta cttcgctacg gaaaaggctg actttgtaaa
     6481 ggtttcacgt ttcaaggacc ccgctacact ttaactctcg cgactgtgag aataatcacg
     6541 cggtatattc tcagagatgc gtcttcagct gggttacgat gcccacgctt cctctaaaac
     6601 ccgtcctggc tcgttaaaat attgggcatg attcttgcct ttgttggcgt taagggacaa
     6661 gaggagaaac agatggcgat tatcaaaaaa cttaaagaac tgttcgacga agcgaaggtc
     6721 agttacgaag tctacaatca tccgctggca tataccgcac aagagatcgc gcagcggcag
     6781 catttttccg gagaccagat ggccaaggtg gtgattctca aagtggacgg caagttggtg
     6841 atggtggtaa tcaccggtaa tcaaaaagtc catttgccta ctgtccgtgc aagcctgggc
     6901 gctgatgaag tgaggctggc aacggaagac gaatttattt ctaaattccc agattgcgaa
     6961 atcggcgcga tgcctccgtt cgggaacttg ttcggcgtaa tcgtatatct ggaccctgcc
     7021 gtagccaagg acgaattaat ttacttcaac gccgggaacc acgtccaaac ggttcggatt
     7081 cgctacaaag atttcgcaag actggtaaat ccagaggtcg tgcgattgac cgaagataga
     7141 acaaagcacg cggcctaggt ccatccaggc ccagtcgatc attcgtatat agaatgcggt
     7201 atgcggagcg ggcttgggct gttcaacaac gagagagcga taatataaga gcaaagctcg
     7261 cgctgaggag cctttttgcc gggtccactc aaagagtgta ctcctcgctc tgtcctgccg
     7321 atttatgcgc acgctttctc gacgcggtca ccgatcccgt gatcgtaggt cggtccaaaa
     7381 tacttggcca acgagtagga ctgatagcaa ttggcgagcg ctcggcaatc cgcttcataa
     7441 tcttccatat aaaattcatt ggtttcaaaa cttgatatag ccctcaatcg atcgctgctg
     7501 agggttttcg cgcttgcgac agctcccgct cttaccagtg cccaaaatag agacatgttt
     7561 tgttgaaaag tcatttcttt cttatgttgc attgcctcag cgggccatct taatgggtcc
     7621 gcgttctagt tgagaatccg gtccttcagg gccacggggc atgcagatat ggcggcggca
     7681 cagccacggt tattgatttt cgtctttgtg atgtttcctg gcacgcgtaa tgctcgtaac
     7741 caattacgtg atgagaaaaa aatttaagga aggatgcaaa tctatgatca gcagaaggaa
     7801 cgattacctg gttaaggcac tcaatcattt tgcttcgctc acggctgtgt taagtcatcc
     7861 cgcgccttta gcgaaatcgt taacacaggg atataacggt catcgaccaa cccaaaagaa
     7921 gtctcctggc acacaccggc ggcgaaaatc tggtcgtact gactgctgcg tcaccccgtt
     7981 cggaaagatt taagccgcga ttgtttaacg ggccgaacgc cactcaaaca tattgggcgg
     8041 agattcagct aaaagagtgc aagctttgtt ctcgaagtgg acggtgttcc ggtaagaaaa
     8101 gtgagggatt ttccactggc tttgacccaa taatacgact cctgacattg aatgagaaaa
     8161 gctggaacgt taataagcgt ctgcttgctc gtgctgcaag cctcttcctg cgctaacttc
     8221 ggcgcttggg tgcgccgata cacctatcca ccgacgtttc gttacattac cgacgaacaa
     8281 ctgcggtcaa ccatgtggca gttggcttat cattcgcgtg aactaaatga tctcatggcg
     8341 cgggaggcat tcgcatctca gcgcgctgaa gtaatgcagc atcttcgcct catggagcag
     8401 gcgatgctcg atttgaatcg aactggctgg ccgaccaatc acccattagt cgatgccaag
     8461 aggtcaaact tcttgcgcga tattttaaat gcgcaggagg cggtcagtcg cgattcacct
     8521 agttttttgc ttgctggcgc agtttctggg gcttgcgcct attgccatcc taaccgatga
     8581 ccaacctgag catagtccgc ttgcagaggg tcgaactgtg attgctcgat tgctccaccg
     8641 accgctactt taaaattata aatggcttct cgtaacaggc cttttttaag gcacttcttg
     8701 ctcaaaataa ttgggttgct tggttcgagg gggcggcggt ttcaccaaaa gagacggatc
     8761 cttctgaaag gagatgactc tgacgcggag aatgaaatgg tccacttgga taagcagagt
     8821 tctcggcttc aacgatcccg aggggtggcg attctgaaag tcttatgcca tcgccatgtt
     8881 tgagaaagca gcttgccgcg catgccgaac caatgcaatt atccgaagtt tcgcgatggg
     8941 tttatcggtc tggcggcaga ggttgagata cacgttgaat cgatcgatcg cgcaatcgat
     9001 tatcaagcgt agttctcatg gatctacgtt gaattcagcg acctaaccag ttatgtgaac
     9061 cggtcatggt cagcacaaat ttaacgatca gcgtagcaac gatggctagc accagatagg
     9121 tgatttcggc ttcatttgtc atcacgacct ccgcgaacgt ttcatcataa cggactcgac
     9181 aggcaccgta tccccgcact aagggtgcga atgatcatgc ccgtactgtg attctatgcg
     9241 caaagccggt gccaactcgg tgccggctaa atcatctgcc gctcgagttt gggtgttgtg
     9301 aatttcgttt agtttcaata agttattccg aaaccggcga tttacttcag ctctgattga
     9361 gaaaaccatt tgcagttctg ataatttttt cgggggaagt tcgtgagatc gataaatggt
     9421 tcgtcttgta aagtttttgc cggatgagtg aagacctagg gaacaaacca tgcgggaatt
     9481 gagcgacgtc aacccaaatc ttcagaccga tatcagcgcc aagcaacaga gcttcgagga
     9541 gtccgagctg ctgttaaatt tatacagggc gcttattgaa aatcgtgatc taggctcggg
     9601 cctccgcgca gccgttaaaa tcgtatgtga atttaccaga tggatagtcg gtactgcttg
     9661 gcttccttcc aaggacgaaa caaaaattgg gctatgctct ttttggcatc aagacgatcc
     9721 gaagctcgcg gagttcatcg tcagttgtca acaccaacag ttcgcccgca acgttggcat
     9781 cctcggccga gtgtggcacc gccaaaaata tgagtggacg cgcaatctcg cggtcgagcc
     9841 taccgattca tttcccctgg cgccggccgc ggcaaacgcc ggtcttaagg cggcgttcgc
     9901 gcttccgatt atgcacaatc atgatgtcga cgccgttttg tgtttccatg cccgcgaggc
     9961 aagggaggaa gacggacgtc tcatcgaagt tatttctcgc atcgcaaatc aattgggatt
    10021 cgcgctgcag cacaaaggtc tcgaggaaga acttttgcaa cagcaggcgc tcgtgcttcg
    10081 aacccatgga aatctggagg aagaggttga acggcgaacc gccgagctca ggttggccaa
    10141 tcaaatgctc caggcagaag ttagtggacg cgagctaaat gagcgtcgaa tgcaagagcg
    10201 aacgcgccag caggaggccc tagccaatct cggcaggcag gctttaggcg ggacggattt
    10261 gtcggcgctc atgcatacga ttactgagct tgtggcaact tccctgaatg tcgagttttg
    10321 caaagtgtta gaactgttgc ccggaagcga tactttaatc ctacgcgcag gcgtcggttg
    10381 gaaagtagga ttggtcggcc gggcaacggt gagcgcaggc accaactcac aagctggcta
    10441 taccttgctt tgcaaggagc cggtgatcgt gaaagatctg ccgacggaag aaagatttag
    10501 cgggccaccg ctattatgtg atcacggagt gataagcggt ctaagcgtta taattggaca
    10561 aagcgaccgg ccgttcggtg ttctcggagc gcataccgcg agggcacgag tattttcgca
    10621 agatgacgtt cattttttgc aggcgatagc gaacgtcttt tcccaggtga tcgagcacaa
    10681 tctcgctatc gaagaactcc accgcaacgc gacttggcta aagcgtctca ttgaaaccac
    10741 gcaagacgcg gtcgtttcca tcgaccggcg cggttgcatc gttctgttca acgccgccgc
    10801 ggaacgagta ttcggctatc gcgcggacga ggttgtcgga cgcaaggtca acgatctcat
    10861 ggctgaaccg tacgcgaccg agcatgacgg ctatatcgca cgctatgaag agaccggcga
    10921 gccgcgcgcc atcggccgga tccgctcagt cgaaggacgg cgcaaaagcg gcgagacttt
    10981 tccactcgag ctttcggtga cgcaagtcgc gaccggcgaa agcgaaaaag ttcattatgc
    11041 cgctttcatc cgggatattt ccgaagtgag acgcggccaa gcttggctgc aaagcttgat
    11101 cgaagcgact caggacgccg tgctgtccat cgatcggcga ggacgcatcg ctttattcaa
    11161 tcctgccgct gaacggatat tcggctatac gcgcgacgaa gtcatcggac aaaaggtgaa
    11221 tatgctgatg gccgagccgt acgcgacgga gcacgacgaa tatatcgcgc gttatgaaaa
    11281 aaccggtgaa gcccgcgcta tcggtcgaat tcgttcagtt accgcccggc gcaaagacgg
    11341 ggagcctttt ccgatcgaac tatcggtcac tgaaatagcc atggaccaag aggttcatta
    11401 tgcggcattc attcgagata tctctgagaa aacgaaattg cagggtcaat tagtcgagag
    11461 cgagagatta gccgccattg gcagcacggc ggcaaagatc ggccacgagc tcgccaaccc
    11521 gctcaatgga atgtccctca ctgtacagct cctcgaacaa cggatcacca ggctaaatct
    11581 tgatgcaatt agtcaactca ccccgaccgt gaagcggctt agggatgaaa tctcgcgtct
    11641 gcaaaaactg gccggtgagt tcgcgacgat tgcgagaaaa gagaaatatg tgttccgccc
    11701 ggttaacctg atgcagcttg tcgacgacgt agtcaaactt caagcgctgc attttgcccg
    11761 aaatggggtt gaaatacaga tttcgttggc cgaggagctg ccgagcataa gcatcgatag
    11821 cgataagatg aagcaggcgc tgctcaattt aataaagaac tccgccgaag cgatgcctcg
    11881 cggcggcagg attattttca acgcgtttgt ggccgacggc gcggtcatat tggaaatcac
    11941 cgatacagga gacggcatcc cattggacat cgatgcattt gaaccttttc tgaccaccaa
    12001 gcgagaagga actggagtcg gtctggttat cgttcggcaa atcctcaccg cgcataacgg
    12061 aaaaatatct taccgaagta aacccggaga gggtacgacc ttccggatag aactgccgct
    12121 aacactctga ttcaaataaa attggactgg cgtaggtatt gcgtttctgc tttcagacag
    12181 cattttcatt agcttaattt aagatatgtt gcgcagacag gatttttacc gcgagagttt
    12241 cgcaatagtg cgaaaagaag aaggcccaca ttgattggaa cgatactggt ggtcgaggat
    12301 caagactccg cccgcgagag cttggtcgat ttactaaaga acgagaattt tgaagttcat
    12361 gctgccgccg acggcaaagc cggtattgag ctcgttgacc agcatgatct cgatctggtg
    12421 ctcaccgatc tagccatgcc gggcgcggac ggcattgccg tgttgcgtca cattcgtgac
    12481 gtctcgccgc agaccttggt tatattgatg acggcccatg cgacggtcga taccgcggtc
    12541 gaagcgattc ggctcggcgc ccaagactat ttgctcaagc cgcttatttt tgaagatgtg
    12601 ctgcgcaaag tccagcatct gatgaatcac cggaagctcg cctgggagaa tcagttgctg
    12661 cggcgcgaga tcaacacgcg attcagtccc gagcgaccgc tcgggcgcag ccaggcgatg
    12721 caagatatta cgcacttaat aaaaaaagtc gcgccgactc cgacgacggt tctcatcacc
    12781 ggagagagcg gggtcggcaa ggaagtggtt gcgcggacga tacataccgc gagtcgattc
    12841 aatgaaaatg tttttttacc ggtcaactgc agcgcgatac cggaaaatct tctcgaaagc
    12901 cagctttttg ggcatatcaa aggctccttt accggcgcgg tgaattctca ggaagggctg
    12961 tttcagagag cgcgcggcgg cacgatcttt ttggatgaga tcggtgaaat gccactgagc
    13021 cttcagccca agctgctgcg cgtgctagag gagaaagccg ttcttccggt gggatcgaca
    13081 aaccccgtaa aggtgaatgt gcgcatcctg gcggcgacca atcgcgacct caagaccgac
    13141 gtcgaagcgg gacgtttccg tgaggattta ttttatcgtc taaacacatt tgagattaac
    13201 attccgccct tgcgcaaaag gctcgatgat ctgccagggt taattgaaca cctcgttcag
    13261 cgtcacaacg tggaaatgaa cacgagctat aaaggcgttg atagcgctac catgcgaatt
    13321 ctcatgtcgt tcccctggaa gggcaacatc cgcgagcttg acaatgttct ggagcgagcg
    13381 atgattctcg gcgatggcga ttggattaag ccggcggatt tgcccggcca tcaagccgat
    13441 gccggggaat tcaattccga ggataacctt agcgcggcgc tcgatttgta tgagaagagt
    13501 cacattgagc gcaccttgaa taaggccgcc ggcgataaaa tgcgcgctgc tgagcttttg
    13561 ggattgagtc tttcaacgct gtaccggaag cttgagaagc tggcatagat atctaattcc
    13621 cgttggttgc agcggcacgc ctcgaaaggt gacttcagga tgatcttcgc cccatcccga
    13681 agcattgcca aactttatgt tgctcatgcc gatactcgcg aacaattaga tctcggcctc
    13741 cagatacatt gttgcggcaa ccaggcgatt tgaagcccat tggcccggtt tcgagtcgtc
    13801 ag
//
LOCUS       DASQKS010000656         7998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3748112, whole genome shotgun sequence.
ACCESSION   DASQKS010000656 DASQKS010000000
VERSION     DASQKS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3748112"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1237
                     /locus_tag="VE170_17015"
     CDS             278..1237
                     /locus_tag="VE170_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17196.1"
                     /translation="MLTQAAGAQERLRVGLSSVSATQGAMWAAEERGFFKKYGIDPEV
                     IVIGGGASRVVSSLLAGEIQFSVGGGDAVIRAALKGGDTVLAVSPLKTGLQRLMVRPE
                     IRTPADLKGKKIGVTRFGSASHWVLQMFLRKWNMRNDDIQILQLGSSPANLASLDNRA
                     IDGAVLTLPTFFVAEERGYRLLADPADLDIVYLQNSIDTTRSYLKRNRDQALRFVKGY
                     CEGMAFFRKNKRETVAIMQKKLRIQSAQEKDVKYLEKSYDLLATKFYNLIPYATPKAV
                     DATLDFVALDEPKAKGADPKFFLDESLVRELETSGFFKNLYAN"
     gene            1350..2225
                     /locus_tag="VE170_17020"
     CDS             1350..2225
                     /locus_tag="VE170_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ17197.1"
                     /translation="MADYKEIIYQKQRGGVLITLNRPEALNAITRPMLKEFHDALDQA
                     EADPEIRAVVITGAGRAFSSGFDQAPGSGRRRDIEWPYGIQTGMSAADLMNYWSHDDV
                     NLVRIFEMTKPVIGAVRGWAMGGGCWLALYTHITIAAEDAVFAQPEVRHGSNTTFMWT
                     LLAGFKNALRYGLVGDHVDAKEALRIGLVNKVVPVEDLLEECFAIVERIAHVPPETVK
                     INLAISTMGLQMMGLRDALHMDETLSIPAHMMLNEEFRRPLDETRAKEGTKAYLQKRD
                     GPFQPEPFGPRARKK"
     gene            2277..3266
                     /locus_tag="VE170_17025"
     CDS             2277..3266
                     /locus_tag="VE170_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17198.1"
                     /translation="MNLKFPGWILPTLLLTTAFGVDAQQPGKIFRVGFLDNGTASGSS
                     VLVEAFRQELTKLGWIEGKNISIDYRYAEQKSDRLPELAADLVRLKVDLIATAGVTSA
                     VAAKKATTAIPIVMVSVSDPVGADLVASLARPGGNITGLSSLSTELNTKRLEILKDAV
                     PRLLRVGLLWLSGTGIGQVLQLNELRPAAVALKLKLEEIETQADARGLESAFQSAKQK
                     QTGAITTTATRQFFAERKRIVELAGKYRLPAIYFQKEFVDEGGLMFYGTDFDDLYRKA
                     AHYVDKILKGTKPAELPVQQATKFEFVINLKAARQIGYTIPMGLLERANQVIK"
     gene            3393..5561
                     /locus_tag="VE170_17030"
     CDS             3393..5561
                     /locus_tag="VE170_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYJ17199.1"
                     /translation="MNVQSTTWRDLTPLIAPKSVAIIGASQRGADANLAREPRGNRVI
                     RNLKTFGFEGRIVTVNPKYTEVMGYPCYPDVASIPEPVDCLVSAVPNRHVPDLLESAA
                     KAGVRAAVVFAAGFGEIGGEGKARQARLEALSKERGFLICGPNCYGVLNVFGKAPLFA
                     STIPAGFLAGPVALISQSGGLSTTIANALMLNRHVGLSHIISCGNQAGATLEEYINYF
                     VEDENTRVIAVFAEGFKQPEKLLPVAHKAAERNKPLIILKGGRSDVSKRAAATHSGSL
                     AGAAEVVDAVFRQGGIVSVRSINELIDSVSVFSCDGFVKKYRGGRGIGVLSGSGGECT
                     LVSDAASNAGIEVPELTEATKSQLQAAVADFGNMNNPLDGTGAMFDDDTIFPRLLQGL
                     VDDANIDFVTINLEANDPRPKELKSGTRFSAVIEKAAANSDKPIAIFSSVVGGPVDPD
                     ILLPLRNAGVPLMEGAECTTAALRNLAEYHEFQKSRRTNAATSIVAATHGKLPAGILG
                     AEAAFRLFEEFKIPVVPTVLTRGADEATAAADKMGYPVVLKIESGQITHKSDVGGVAL
                     KLASAAEVRDGYSRIMQQVAAKAPQAKLDGVVVQRMASEGVEMILGIKRDPLFGLVVL
                     CGLGGILVEVLKDVAIGIPPLSRQQASEMIQRLRGAAILGGVRGKPPADVDALCDAIV
                     GLANLAVSLGDQLNGIDINPLIVLPRGQGVVAVDALVEIQ"
     gene            5783..6820
                     /locus_tag="VE170_17035"
     CDS             5783..6820
                     /locus_tag="VE170_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17200.1"
                     /translation="MRAELRRRDGRALKPEKKKHIASLILMVIGAVVVCSTDLPAQSK
                     PLKEVRVPYALGGSTGFFWVAHKSGAFEKYGLKVLPIFMRGGREAVQALISRDVSIMQ
                     QGSSGVITAWAQGAKDLVAIGATGNKLDYIFVSTPAIKKPADLKGKRIAISQLGASTD
                     FIARIALRQLGLNEKEVTLVSLGAQGERWAALAGGHVDASLFQPPVTLRARKLGMPIW
                     IDFAKIDYEYVTAGPVTTRSYIKAERDTVMNFMRGLADGMEFYRDDRNKDTVIKYLGE
                     FYRSTNAEELEETRRVYSQVSTGLPVVTVKAMENMIASDRVLSAANVNAMDVLDLSFL
                     KQLEEERKIKK"
     gene            6938..7816
                     /locus_tag="VE170_17040"
     CDS             6938..7816
                     /locus_tag="VE170_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ17201.1"
                     /translation="MADYKEILYEKQRGGALITLNRPEAMNAISRSMIKEIHQALDDV
                     EQDPQVRAVVLTGAGRAFSAGMDQGNASGRRRDLHWPYGIVTGETAASVIDSWRADPR
                     NFRRMWEFGKPIIGAINGWAMGAGSWLTLFTHITIASETAVFSQPEVRHGSNTSFMWT
                     LLGGYKNALRYGLTGDHIDAQEALRIGLVVKVVPPDQLIDECFKIVERIAHVPPETVK
                     INLQVATMGLDMMGLRDALTLDNQLSAPAHVMLREELRKPLDDARANKGIREYLQMRD
                     GPFQPEPFGPRSKKRD"
BASE COUNT         1806 a         2353 c         2254 g         1585 t
ORIGIN      
        1 gcaatcgatg gtccaacgca tggcgcaact gaacatgatc gacgcgaaag ccgcacagtc
       61 cacgcccacc agcgcctact tcgacaactc tttcgtcaac gagctcaagc aatccggttt
      121 cctcgccagc gtctggaagt agctcgtcag acgcgaagtt tttcttgttg cgtcgcttga
      181 cgcccaaccg cgctttctac tagttaataa ccatacgttt cggagaacct tgatgcaccg
      241 cagactttca acctgttttt ttgcctacct cgtccttttg ttgactcaag cagcgggcgc
      301 ccaggaacga ctgcgcgtcg gcctgagctc agtaagcgcc acccaggggg ccatgtgggc
      361 ggccgaagag cgcggctttt tcaagaaata cggcatcgat ccagaagtga ttgtcatcgg
      421 cggcggcgct tcccgcgttg tcagctcgct gctcgccggt gaaattcaat tttccgtcgg
      481 cggcggcgat gcggtgattc gcgccgcctt gaaaggcggc gacaccgtgc tggccgtctc
      541 gccgcttaaa actggcttac agcgcttgat ggtccgtccg gaaatccgaa ctccggctga
      601 cttgaaaggc aagaagatcg gcgtcacccg cttcggttcg gcgtcccatt gggtgctgca
      661 aatgtttctg cgcaagtgga acatgcgcaa cgacgatatt caaattctcc agttaggctc
      721 gtcgccggcc aatctcgcca gcctggataa tcgcgccatc gacggcgccg tgctgaccct
      781 gccgacgttt tttgtcgccg aagaacgcgg ttaccgcctg ctcgccgacc ccgccgacct
      841 ggatatcgtt tatttgcaga actctatcga caccacgcgc agttatttga agcgcaaccg
      901 cgatcaggcg cttcgcttcg tcaaaggcta ctgcgaaggg atggcctttt ttcgaaagaa
      961 caaacgcgag accgtggcga tcatgcaaaa aaagctgcgc atccaatcgg cccaggaaaa
     1021 agacgtcaag tatttggaga agtcatacga cttattggcg accaagttct acaacctaat
     1081 cccttacgcg acgccgaaag ccgtcgacgc gaccctcgac ttcgtcgccc tcgacgaacc
     1141 caaagccaaa ggcgccgacc ccaagttttt tctcgatgaa agcctggtcc gcgagctcga
     1201 aaccagcggc ttcttcaaaa atttatatgc gaattaatcc gatatcaaaa atgattggag
     1261 agatggagta gtggagtgat gggctccgga cccgcaaaca ctccagtaca ccaccaccct
     1321 attatctcaa ttccccataa ggagatcgaa tggccgacta caaagaaatc atctaccaga
     1381 aacaacgcgg cggcgtgctg attactttga atcggcccga agcgctcaac gcgatcaccc
     1441 ggccgatgct gaaagaattc cacgacgcgt tggaccaagc cgaagccgat ccggagatcc
     1501 gcgctgtcgt gatcaccggc gcggggcgcg ctttcagttc cggcttcgat caggcgcccg
     1561 gcagcggccg gcgccgcgat atcgaatggc cttacggcat ccaaaccggc atgagcgccg
     1621 ccgatctgat gaactactgg tcccacgacg atgtgaatct agtgcgcatc ttcgaaatga
     1681 ccaaaccggt gatcggcgcc gtgcgcggct gggccatggg cggcggttgt tggttggcgc
     1741 tttacacgca catcaccatc gccgccgaag acgcagtctt cgcccagccc gaagtgcgtc
     1801 acggctcgaa cacgacgttt atgtggacgc tgctcgccgg tttcaaaaac gccctgcgct
     1861 acggcctggt cggcgaccac gtcgacgcca aagaagcgct gcgcatcggc ctggtcaaca
     1921 aagtcgtgcc ggtcgaagac ttgctcgaag aatgtttcgc catcgtcgaa cgcatcgccc
     1981 atgtgccgcc ggaaacggtc aagatcaacc tcgccatctc gaccatgggt ttgcagatga
     2041 tgggactgcg cgacgccctg cacatggacg aaaccctgtc gatcccggca cacatgatgc
     2101 tcaacgaaga gttccgccgc ccactcgacg aaacccgcgc caaagaaggc accaaagcct
     2161 atctgcaaaa gcgcgacggg ccgtttcaac cggaaccctt tggcccgagg gcgaggaaga
     2221 aataaaagta atcggctatc agggatcggc tatcggagtg aaggaaaagt gagagattga
     2281 atctaaaatt tcccggttgg atcctgccaa ctctgctcct gacaactgcg ttcggagtgg
     2341 acgcgcagca gccgggcaag atcttccgcg tcggtttcct ggataatggc actgcttctg
     2401 gcagctctgt cttggtggag gcgttccgac aagagctgac gaagctggga tggatcgagg
     2461 gaaagaatat tagcattgac taccggtatg ccgaacaaaa gagtgaccgc ttgcctgaac
     2521 tggcagcgga cttggttcgt cttaaggttg atctgatcgc gaccgcaggg gtcacctcag
     2581 cggtggcggc gaagaaagct actactgcca ttcccatcgt gatggtaagc gttagcgacc
     2641 ccgtgggggc agatttagtc gctagtttgg cgcggcctgg aggcaatatc accgggctct
     2701 cgagtttatc gacagagcta aataccaaac ggctagagat actcaaggac gcggtcccaa
     2761 ggcttctccg agttggactt ttgtggctgt cgggaaccgg cataggacaa gtccttcaat
     2821 tgaacgagct cagacccgcc gctgtggctc tgaagctaaa gttggaggag atcgaaactc
     2881 aagccgacgc cagaggtttg gagagcgcat ttcaaagcgc aaagcagaaa cagacgggcg
     2941 cgattacgac gaccgccact cgtcaatttt tcgccgaaag aaagcgcatc gtcgagcttg
     3001 ccgggaaata ccggttgccg gccatttact tccagaagga atttgtcgat gagggcggcc
     3061 tgatgtttta cggcacggat ttcgatgacc tgtaccgcaa agccgcccac tatgtggata
     3121 agatccttaa gggaacgaaa cctgctgaac tgccagtgca acaggcgacg aagtttgaat
     3181 tcgtcatcaa cctgaaggcg gcaagacaaa tcggttatac gattccgatg ggattgctag
     3241 agcgggcgaa tcaagtgatt aaataggcag acataagaaa gaaaatgagc gatggagtat
     3301 tggagtagtg gcgtgatggg ttccggacat ccgagacggc gtcgtactcc aacactccat
     3361 ttttcggcaa ctccaccaaa taggatttat ttatgaacgt acaatctacc acttggcgcg
     3421 atctcacacc gctcatcgcg cccaaatccg tcgccatcat cggcgcatcg cagcggggcg
     3481 ccgacgccaa tctggcacgc gagccgcgcg gcaaccgggt gatccgcaac ttgaaaactt
     3541 tcggcttcga gggccgcatc gtcacggtca atccgaaata taccgaagtc atgggctacc
     3601 cctgctatcc cgatgtcgca tcgattcccg agccggtgga ttgcctggtc tcagcggtgc
     3661 ccaaccgcca tgtgcccgac ctgttggagt ctgcggccaa agccggcgtg cgcgcggcgg
     3721 tggttttcgc cgctggcttc ggcgaaatcg gcggcgaagg caaagctcgt caggcgcgct
     3781 tggaagcgtt gtcgaaggag cgaggttttc tgatctgcgg ccccaactgc tacggcgtat
     3841 tgaacgtgtt cggcaaagcg ccgctgttcg cttcgactat tcctgccggc tttctcgccg
     3901 gcccggtggc gctgatctca caaagcggcg gcctgagcac gaccatcgcc aacgcgctca
     3961 tgctcaaccg ccacgtcggg ctcagccata tcatctcctg cggcaatcaa gcgggcgcga
     4021 cgctggaaga atacatcaat tattttgtcg aggacgaaaa cacccgtgtc atcgcggtat
     4081 tcgccgaggg cttcaaacaa ccggaaaaac ttctgccggt ggcgcacaaa gcggccgaac
     4141 gcaacaagcc gctaattatt ttgaaaggcg gccgctccga cgtttccaaa cgggccgccg
     4201 cgactcactc aggatccctt gccggcgcgg cggaagtggt cgacgcagtg tttcgccagg
     4261 gcggcatcgt ctcggtgcgc agcatcaacg agctcatcga cagcgtgagc gttttctcct
     4321 gcgacggttt cgtcaagaaa tatcgcggcg gccgcggcat cggcgtcctg agcggttcgg
     4381 gcggcgagtg caccctggtt tccgacgcgg catcgaatgc cggcatagag gttcccgagc
     4441 tgaccgaagc gaccaaatca caattgcagg cagcggttgc cgacttcggc aacatgaaca
     4501 accccctcga cggcaccggc gccatgttcg acgacgatac gattttccca cggttacttc
     4561 agggtttggt cgacgacgcc aatatcgatt tcgtcaccat caatctcgaa gccaacgatc
     4621 ccaggccgaa agagctgaag agcggcacgc gcttttccgc ggtcatcgag aaggcggcgg
     4681 cgaacagcga caaaccgatc gccatcttca gctccgtggt cggcggtccg gtggaccccg
     4741 acattttatt gccgttgcgc aacgccggag tgccgctcat ggagggcgcc gaatgcacca
     4801 ccgccgcctt gcgcaatctc gcggagtatc atgaatttca aaaatcccgg cgaaccaacg
     4861 ccgccacgtc gattgtcgcg gcaactcacg gcaaacttcc ggcgggaatt ctcggcgccg
     4921 aagcggcttt ccggctattc gaagagttca aaatacccgt ggtgccgacg gtgctcactc
     4981 gcggcgccga cgaagccacc gccgccgcgg acaagatggg ctacccggtg gtcttgaaaa
     5041 tcgaatccgg ccaaattacc cacaagagcg atgtcggcgg cgtagcgctg aagctcgcca
     5101 gcgcggcgga agtgcgcgat ggttattcac gcatcatgca gcaagtggcg gcgaaggcgc
     5161 cacaagccaa actcgacggc gttgtggttc agcgcatggc ctctgaaggc gtggaaatga
     5221 tcctcggtat caagcgcgat ccgcttttcg gccttgtggt gctatgcggc ttgggcggca
     5281 tactcgtcga agtgcttaag gacgtcgcca tcggcattcc accgctgtcg cgccaacaag
     5341 ccagcgaaat gatccaacgc ctgcgcggcg ccgccatcct cggcggcgtg cgcggtaaac
     5401 cgcccgcgga tgtcgacgcg ctgtgcgatg ccatcgtcgg cttggcgaat ttggcggtca
     5461 gcctcggcga ccagctgaac ggcatcgaca tcaatccgtt gatcgtattg ccccgcggcc
     5521 aaggcgtcgt cgccgtcgac gcgctggtgg agattcaatg atcatgcctg ctaaaatttc
     5581 cgctgtatcc cctcttcccc ttattccccc ctttgaaaaa ggggggccag gggggatttt
     5641 gacaatgcgc accggaaaat tcccctcatt cccccttttc caaaggttga agcggaattc
     5701 actgtcactg acaaccacca cggatccgtc attcccgcgc aggcgggaat ccaggtttga
     5761 tgccgccgaa ccaaactgga atatgcgtgc cgagctgcgc cggcgtgacg gacgggcatt
     5821 aaagccggag aagaaaaaac acattgcttc gctgatccta atggtcattg gtgctgttgt
     5881 cgtttgctcc accgaccttc ctgcccaatc taagccgctc aaagaagtcc gcgttcccta
     5941 tgccctcggc ggctccaccg gctttttttg ggtcgcgcac aaatcggggg cctttgaaaa
     6001 atacggcctc aaagtcctgc cgatcttcat gcgcggtgga cgcgaggcgg tgcaagcatt
     6061 gatctcgcgc gacgtctcga tcatgcagca aggaagttcc ggcgtcatca cggcgtgggc
     6121 gcagggcgct aaagacctgg tagccatcgg tgccaccggc aacaaactcg attacatctt
     6181 tgtctcgaca ccggcgatca aaaaacctgc cgatttgaaa ggcaaaagga tcgccataag
     6241 ccaactcggg gccagcacag atttcatcgc acgcatcgct ctacgccaac tgggacttaa
     6301 cgaaaaagaa gtgaccttgg tcagtctcgg cgctcaaggc gagcgttggg ccgctctcgc
     6361 tggcggccat gtcgatgcct cgttgtttca accgccggtc accctgcgcg cgcgcaaatt
     6421 gggcatgccg atttggatcg attttgcaaa aatcgattat gaatacgtca ccgccggacc
     6481 ggtgacgact cggtcctaca tcaaagccga gcgtgacacg gtgatgaact tcatgcgcgg
     6541 cttggctgac ggcatggagt tttaccgcga tgataggaat aaagataccg tgatcaagta
     6601 cctgggcgaa ttttaccgct cgaccaatgc cgaagagctg gaagaaaccc ggcgggttta
     6661 cagccaggtt tcgactggct tgccggtcgt gacggtcaaa gcgatggaaa atatgatcgc
     6721 cagcgaccgc gtgctgtcgg cggcgaatgt gaatgcaatg gacgttctcg acctgtcgtt
     6781 cctaaaacag ttggaggaag agcggaaaat taaaaaatga aagtttaaac cgttcaatcg
     6841 cttcgctccg ttcaagccgt ttaaatcgtt ttccgcacac gtccggctat ttgaacgttt
     6901 tgaacggctt gagcgatttg aacccatagg agacccaatg gccgactaca aagaaatctt
     6961 atatgagaaa caacgcggcg gcgcgttgat cacgctcaat cgcccggaag cgatgaacgc
     7021 catcagccgc agcatgatca aggaaatcca ccaggccctc gatgacgtcg agcaggatcc
     7081 gcaagtgcgc gctgtcgtgc ttaccggcgc cggccgggcg ttttccgccg gcatggatca
     7141 gggcaacgcc tcggggcggc ggcgcgatct gcactggccc tacggcatcg tcaccggtga
     7201 gactgccgcc agtgtcatcg attcctggcg cgccgatccg cgcaacttcc gccgcatgtg
     7261 ggagtttggc aagccgatca tcggcgcgat caacggctgg gccatgggcg cgggctcctg
     7321 gctgaccctg ttcacccaca tcaccatcgc gtcggagacc gccgtctttt ctcagccgga
     7381 agtgcgccat ggctcgaaca cgagctttat gtggaccctg ctcggcggct acaagaacgc
     7441 gctgcgctac ggcctcaccg gcgaccacat cgacgcccag gaagcgctgc gcatcggtct
     7501 ggtggtcaaa gtcgtgccgc cggatcaact gatcgacgaa tgtttcaaga tcgtcgagcg
     7561 tatcgcccat gtgccgccgg aaaccgtcaa gatcaattta caagtcgcta ccatgggcct
     7621 cgacatgatg ggtttgcgcg acgccttgac cctggacaat cagttgtccg caccggccca
     7681 tgtcatgttg cgcgaagagc ttcgcaagcc gctcgacgac gcccgcgcga ataaaggcat
     7741 tcgcgaatat ttacaaatgc gcgacggccc attccagccg gagccgttcg gaccccgttc
     7801 caagaagcgc gattgagcaa gcggttcgtc gaaaatgttc aagccagtca aatcgttcaa
     7861 gcagcaaaag taggagaaca agacatgtca cagtatcaaa caattcttta ccagccgcag
     7921 cgtaaaggcg ttctgatcac gctcaatcga cccaatgcct tgaacgcgat ggatcaagaa
     7981 atgatgagcg agctcgat
//
LOCUS       DASQKS010000657         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_796186, whole genome shotgun sequence.
ACCESSION   DASQKS010000657 DASQKS010000000
VERSION     DASQKS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796186"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..638)
                     /locus_tag="VE170_17045"
     CDS             complement(396..638)
                     /locus_tag="VE170_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17202.1"
                     /translation="MLNWLDLLQWPAMMTNVLAAWLIASQRELRRKIGFYCFLAGNIL
                     WVIWGWHAQAWAMVVLQVCLVGLNLRGVKKNDASNG"
     gene            843..2531
                     /locus_tag="VE170_17050"
     CDS             843..2531
                     /locus_tag="VE170_17050"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYJ17203.1"
                     /translation="MEMPRKRAIVTAAGLLAVGFALYLRRSPTLRKLVKLFARATAHQ
                     LRPVLRPIEPGARALIFAFDGVGADALYNAIRDGHCPHIQNLLGGDCGAGTFEHGYAI
                     PNAMSILPSTTMAGWSSIFTGQPPAFTGVTGNEWFVREEMRLYAPAPVSVTDIADTAR
                     MLADGLVGNAIRVPTLYELLDRRAHVALAPVHRGADLFTITDASILADLFGAVAKGTL
                     GNTTVKQEVYQEMDENSVEALLEAIAKYGVPNLQVAYFPGIDLFSHIAEAPLDIQQIY
                     MEKTLDPAIGRVLEVYQRAGVLDQTYVLFVADHGHTPVLNDDHHSLYTTGDHEPPALL
                     EELGFRVRPFVLEPKPSENDYNAALAYQGAMAYIYLADRTAILPNGNYDWRRAPRFEE
                     DVMAVVRAFDRVNRTGQPISQLKGVLDLIFARVPRPVGEHALPFEVFDGERLVAIGDY
                     LKRHPRPDLIRLESRMRDLSAGPYGHRAGDVLLLCRSGLELPIEERFYFSGLYRSWHG
                     SPTRQDSHVPIIVARQNSSGPELARHTQEIVGAEPSQLHIVPLLLHLLRAAEKR"
BASE COUNT          558 a          745 c          746 g          624 t
ORIGIN      
        1 agaaaaccgt ctcttcgatc cgcgcatggg tctcgagctc ggttttaatc tgatcgaaga
       61 tcttttcttg ctccttgccg tcagcctctt cagctttttc gaaaagctct ttgacctttt
      121 gatgatcttg tttaagtagt tctaaagcgt ccatttgttt atctccttaa ttgatttttc
      181 aagctgtata gagcaaatga tagaccatac gataagtgat aggttgcgcg ggtatatgcg
      241 gctgagttgt cccagtccta ggatataacg acggcttggc ggccaacgct gggacaaagg
      301 cgaatagctc tttatgtgat gcttagtttg gggagtagta gaataaagtg aagatcccta
      361 attctatcgc aaaatgatcg cacaattctc aatcttcagc cgttgctggc gtcgtttttt
      421 ttgacgccgc ggaggttgag tccgactagg caaacttgca agaccaccat cgcccatgcc
      481 tgagcgtgcc agccccagat tacccaaaga atgttacccg ctaggaagca gtaaaaaccg
      541 atttttcggc gcagttcccg ctgggaagcg attaaccaag cggcgaggac attggtcatc
      601 atcgcgggcc actgtaagag gtcgagccaa tttagcatag gatcggcggc tgactaacgt
      661 gacaaacacg tcgttggcgg gcagttgggc gcatggcact ttgttctggc atcgagtttg
      721 cgccctcccg cgcgatgaca gcgcgaacag aagcaaccgg tctgccgtgt accggtaact
      781 cttttgaccg gaaaactttg atcgacgagg cggcgccgat cgtttaggtt gggatgtacg
      841 ctatggaaat gccacgcaag cgagcgattg taactgcggc gggcctgcta gcagtgggct
      901 ttgccctgta tctgcgaaga agtccgacgc tgcgaaaact tgtcaagctg tttgcccgcg
      961 ccacggcgca ccagttgcgt cccgtgctgc ggccgattga gccgggtgcg cgggcgctca
     1021 tcttcgcttt tgacggcgtc ggcgcggatg cgctttacaa cgcgattcgg gatggccatt
     1081 gtccgcatat acaaaatctt ctcggtggcg attgcggcgc cggtaccttc gagcatggct
     1141 atgcgattcc gaatgccatg agtattttgc cgtcaacgac gatggcagga tggtcctcaa
     1201 tctttaccgg ccagccgccg gcatttaccg gcgtgacggg aaacgaatgg tttgtgcggg
     1261 aggaaatgcg tctttatgcg ccggcgccgg tatcggtgac tgatatcgcc gataccgcaa
     1321 gaatgctggc cgacggtctg gtcggcaacg cgatccgggt gccgacgctt tacgaactgc
     1381 tcgaccggcg cgctcatgtt gccttggctc ccgtgcaccg gggcgcggac ctgttcacca
     1441 ttaccgatgc cagcattctt gccgatctat tcggcgcagt agccaaagga accctcggca
     1501 ataccacggt caaacaagaa gtctatcagg agatggatga aaactcggtc gaggcgctgc
     1561 ttgaagcgat cgccaaatac ggcgtgccga atctgcaggt cgcctatttc ccgggtatcg
     1621 atctgtttag ccatatcgcc gaggcccccc tcgacatcca gcagatctat atggaaaaaa
     1681 ctctcgatcc ggcgatcgga cgggtgctcg aagtctatca gcgtgccggc gtgcttgacc
     1741 aaacctatgt tttgttcgtc gccgaccacg gccacacgcc ggtattaaac gacgatcatc
     1801 attcgctata caccaccggc gatcatgagc ctcccgcgct gctcgaagag ctgggcttcc
     1861 gagtgcgccc cttcgtgctc gaacccaagc cgagcgaaaa cgattacaat gccgcgctcg
     1921 cctaccaggg cgccatggcg tacatctacc ttgccgaccg cacggcgatt ttgccgaacg
     1981 gtaattacga ttggcggcgg gcgccacgct ttgaagaaga cgtcatggcc gtggtgcgcg
     2041 cgttcgatcg ggtgaaccgc accggacaac cgatcagcca actcaaaggt gttctcgatc
     2101 tgatttttgc gcgtgtgccg cgccccgtgg gcgaacacgc cctgccgttt gaagttttcg
     2161 atggcgagcg gttggtcgcc atcggcgatt atttaaagcg tcatccgcgt cccgatttga
     2221 ttcgcttgga gtcgcgcatg cgcgacctgt cggcgggacc ctatggccat cgcgccggcg
     2281 atgtcctgct cctttgccgc tcgggtttgg agctgccgat cgaagagcgc ttttatttct
     2341 ccggtctata tcgctcctgg catggcagtc caacgcgcca ggacagccat gtgccgatca
     2401 tcgtcgcccg gcaaaatagc agcggaccag aactggcgcg gcatacccaa gaaatcgtcg
     2461 gtgcggagcc gtcgcaactg catattgtgc cgctgctact gcatctgctg cgcgcggcag
     2521 agaagcgata ggaaccataa ttactgaatt cacgtcgttg gattagggac gagcgttcga
     2581 atacttctcg gttcggtcaa ccgcgccgtt attgcgcttc atgcgttttt gcaaccattt
     2641 ccaaacaaat ccaccaatca tcatcacgat cat
//
LOCUS       DASQKS010000658         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3321962, whole genome shotgun sequence.
ACCESSION   DASQKS010000658 DASQKS010000000
VERSION     DASQKS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3321962"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VE170_17055"
     CDS             complement(<1..477)
                     /locus_tag="VE170_17055"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HYJ17204.1"
                     /translation="MKTKSTTRLKELLAGSQLLVMSGGFSPLHARMSEITGYEAFFMS
                     GSQVAAYVYGYPDVGLLGLNEMVEAVRRITNVTDIPVFADADTGYGNAVNVYYTVQAY
                     IRAGAAGLHIEDQEAPKKSGTLAGRRLISVEEAVGKYRAAVAAKQELDADFVVCARC"
     gene            756..1538
                     /locus_tag="VE170_17060"
     CDS             756..1538
                     /locus_tag="VE170_17060"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYJ17205.1"
                     /translation="MADIFLIYARDNSEVAKRLSESLDNIGWSVWWDWQVPPGQTWSG
                     MIEQSLTSMRCMVVLWSTDSIDSEWVKEIAGKGRAQCKLIAALIDDVKPPIGFPAIQA
                     ANLIDWDGTQDFPGFGNLIDALEAQLGKPVKNPDAAPATASEINAPPRWQSSSLALLD
                     STKLRQLANRLSWQFCAKAAVLVIILVAASSQRQVRDAGTTAPAALPATSLDAGEMRP
                     SPPLTIRGPSIAKRREARRSQPPHRARTVAARFLPARSSPSR"
     gene            1588..>2641
                     /locus_tag="VE170_17065"
     CDS             1588..>2641
                     /locus_tag="VE170_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ17206.1"
                     /translation="MAKNVGRVRALIHALLLASLGACTTTSEAPPPALPEVAVEPVVA
                     APAPALAPTTDSGVVRPFEVALTDAANMLFSQIRTSANSEKQLLVIDPLIDGVSGIQS
                     RATTTIASRIGELVRGRYPQFDLQPFSAANVRRSPKVLVGTFTAVNSDGHTTGTREAY
                     RICLVLADLMSSTVVAKATAHSSMLGVDHSPTPYFQDSPAWMNEGPTESYVNTCQTSK
                     PGEPIQPGYLDSILAGTLISDATAAYDAGRYADALDRFNSALELPTGHQLRVFNGLYL
                     ANLKLGRQAAANDGFGRIVGYGLAKNRLAVKFLFKPGTSAFVADKQASAHYSMWMKEI
                     ARRATQNKSCLEIIGHT"
BASE COUNT          579 a          797 c          732 g          533 t
ORIGIN      
        1 gcaacgggcg cagacgacaa agtcagcgtc gagctcctgt ttcgccgcca ccgccgctcg
       61 gtatttgccc accgcttctt ccaccgagat caaacgccgt ccggccagcg tccccgattt
      121 cttcggcgct tcttgatctt cgatatgcaa gcccgccgcg ccggcgcgaa tatacgcttg
      181 cacggtgtaa taaacgttca cggcgttgcc gtagccggtg tcggcatcgg cgaagactgg
      241 aatatcggta acgttggtga tgcgccgcac cgcctcgacc atctcgttca agccgagcaa
      301 accgacatcg ggatagccat agacataggc cgccacctgg gagccagaca taaaaaatgc
      361 ctcgtaaccg gtaatttccg acatgcgcgc atgcaacggg ctaaagccgc cggacatgac
      421 gagcagctgt gagccggcta acagttcttt caaacgagtg gtcgatttgg tcttcatggt
      481 gaccttccat tatcacatag ctagccgccg agaaaagctg gcgcgtagcg taattgccga
      541 gacggcgcca gccccatgac ggtgatcgcg aagtattgca gtacaatttt tgacaagact
      601 gacaaatgtg ttttgctgca aattcttagc gcgcccataa accgccgatt tttagccgcg
      661 cttagagggt ggcgacaatg gcctggttgt tgctccgcta cacttgaaag ttcgttatta
      721 agcagcgttg gtaagcagca tttaggttac gttccatggc cgatattttt ctcatttatg
      781 cccgcgacaa cagcgaggtc gccaagcgct tgagtgaaag cttggacaac atcggctggt
      841 cggtctggtg ggactggcag gtcccgccgg gccaaacctg gagtggcatg atcgagcagt
      901 cgctgaccag catgcgctgc atggtcgtcc tatggtcgac ggactccatc gacagcgagt
      961 gggtcaagga aatagccggg aaaggccgcg cccaatgcaa actgattgct gcgctgatcg
     1021 acgatgttaa gccgccgatt ggctttcccg ccatccaagc cgccaatctg attgattggg
     1081 atggtaccca agacttccct gggtttggca atctcatcga cgcattggaa gcgcagctcg
     1141 gtaagccagt caaaaatccc gacgccgcac cggcaaccgc tagcgaaatc aatgcaccgc
     1201 ctcggtggca atcgagttcg ctggctttac tcgactctac taagctgagg caactagcca
     1261 atcggctatc ctggcaattc tgtgccaagg ccgccgttct ggtcatcata ttggtcgccg
     1321 cgtccagcca acgacaggtg cgtgatgcag gaaccactgc accggctgcg ctgcctgcga
     1381 catctctcga cgctggcgaa atgcgtccat cgcccccctt aactatcagg gggccatcga
     1441 tcgcgaagcg ccgagaagcg cggcgatccc aaccaccgca ccgagcgcgt acggttgcag
     1501 cgagattctt gcccgcgcgg agctcgccga gccgttgacg aatgcagacc gagagacgct
     1561 gagaaaggac tgtccatcat gaaactcatg gccaaaaatg taggccgagt gagagcgcta
     1621 atccatgcgc tcttattagc atcgctcggc gcttgcacta ccacgagtga agcgccgccg
     1681 ccggcgcttc ccgaagtcgc cgtcgaaccc gtcgtagcgg cgccggcgcc agcgctcgcg
     1741 ccgacaacgg acagcggcgt ggtgcgcccc ttcgaagtag cgctgaccga tgccgctaac
     1801 atgttattct cacaaatccg gacttcggcc aatagtgaaa aacagctgct cgttatcgat
     1861 ccgttgatcg acggggtttc gggtatccag tcacgcgcca ccacgaccat agcatcgcgc
     1921 atcggcgaat tggtgcgtgg ccgttatccg cagttcgacc tgcaaccctt ctccgccgcc
     1981 aatgtgcgcc gttcgccaaa agttttggtc ggcaccttca cagctgtgaa tagcgacggc
     2041 catacgacgg gcacacgcga agcctatcgc atctgcctgg tcttggcgga cttgatgtca
     2101 agcacggtgg tagcgaaggc gaccgctcac tccagcatgc taggcgtcga tcattcccca
     2161 acaccgtact tccaagatag tccggcgtgg atgaatgaag gtcccaccga aagttatgta
     2221 aacacttgcc agactagcaa acctggtgaa ccgattcagc ccggctatct cgatagcatt
     2281 ctggccggaa ccctgatcag cgatgcgacc gctgcctatg acgccggccg ttacgccgac
     2341 gcccttgacc gttttaacag cgccctcgag ctgcccaccg gccatcagtt gcgggtgttc
     2401 aatggtctat accttgccaa cttgaagctc ggtcggcagg ccgcggccaa cgatggcttt
     2461 ggcaggatcg tcggttatgg cttggcgaag aatcgtctcg ccgtgaaatt ccttttcaag
     2521 ccgggcacca gcgcattcgt ggccgataaa caggcgagcg cgcactattc gatgtggatg
     2581 aaggaaatcg cccgccgcgc gacgcaaaac aaaagttgcc tggaaataat cggccacacc
     2641 a
//
LOCUS       DASQKS010000659         7789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5417604, whole genome shotgun sequence.
ACCESSION   DASQKS010000659 DASQKS010000000
VERSION     DASQKS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5417604"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VE170_17070"
     CDS             complement(<1..440)
                     /locus_tag="VE170_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17207.1"
                     /translation="MSPVQVKSSAQQKIIGVSTTRSEGREKVSGAAVYATDIVLPNML
                     WAKALRSPIAYGRIDKIDVSKALALPGVHGVVTGADVKGQLIGRKIYDMPVLADEVVR
                     FIGEKVAVVAAETEEIAEAAIALIDVDYEQLDALLDPLEAAQSTA"
     gene            complement(437..1216)
                     /locus_tag="VE170_17075"
     CDS             complement(437..1216)
                     /locus_tag="VE170_17075"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ17208.1"
                     /translation="MSESIGTEQGAAYVDEHYRKVAARWRTRVTESQFMRQLRAGTLP
                     METLRTFFKNWGSYTIEINTVEAASYHKHISFFRKHRDLMARMAEKLADELIHPKPPG
                     HVHVVVETAKALGISEDEIFMAPMLPEFRAKLDFKRSLLWEGTMAEFYAAGATEEQTG
                     YWSADFYKALTAHYGLSREQAVYFSTHEEADLKEHDGVMGHGSFRRLVLRTLIEDGLA
                     HVRPGYTLEYCGLCAVDLHGVCLQAAFEAADKSKIAESDQP"
     gene            complement(1246..1845)
                     /locus_tag="VE170_17080"
     CDS             complement(1246..1845)
                     /locus_tag="VE170_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ17209.1"
                     /translation="MIRYDCLKLLAPRITDELVVTSLSGQKIEWANLSQHHGNLLVGT
                     MGTALGVGMGVAIGLPNRRVIVIESDGSVLLSLFNLPTLANLNPQNLIVLVFDNQVYS
                     GTRISEPTATAGKTDLAMMARGAGIEQATTVRDLDGFQKELDDAVAKNQLRFIVCKVE
                     ESVTHREILRTDLDPIENKYQFVRYLERTEGRAIFRGRG"
     gene            complement(1842..2348)
                     /locus_tag="VE170_17085"
     CDS             complement(1842..2348)
                     /locus_tag="VE170_17085"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ17210.1"
                     /translation="MEAALAKRLVAGLKQAGIDLVTYLPETRLSQILPLLRSDPSFTL
                     VPVASESEAVTIGAGAALTGKQVAIYMECTGLYVSCYTLVSIARRYGVPLLLVVAYLG
                     SMNDQRNNAVYAVSGTYVEPLLDALQIKTQILVNGDRLEDDIQDAVRHMHALKLPVAL
                     VFTGDFTR"
     gene            complement(2392..3387)
                     /locus_tag="VE170_17090"
     CDS             complement(2392..3387)
                     /locus_tag="VE170_17090"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17211.1"
                     /translation="MNLRRLLLTPTVIWLVLTALGLGLAQKAFAQGALEKIVFSIPSR
                     SIAAIDLYVAKERGFFRDEGLDVDIVQIRGNVAIAAALSGQVQATNGVGTVIRALERS
                     EIPLKVLSVSLKRNLFWLVSRKEITSIAQLKNKKLGTTTFGGSQHTAATRLIRKAGLD
                     PDKDITVVVGGDVPAQMQSLVSGVIDAAALSPPTVILARDKYNMNVLGSALEDLFNLQ
                     NGTAVTEKLLRENRPLLRRILRARAKAHRYFLENERGTSEVLAKFQSVDFEVARESYR
                     LAKPAFTTDGIASAKEVDEFLKADAELLKLKEPVAAAKIFDFSLQREVNQELGFK"
     gene            complement(3504..4103)
                     /locus_tag="VE170_17095"
     CDS             complement(3504..4103)
                     /locus_tag="VE170_17095"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ17212.1"
                     /translation="MQRYECLEQLAPLITDQLVVTSLSGQRVEWGHLSKHEGNLLLGS
                     MGNAIAVGLGLALALPNRKVIVLESDGSLLLSLFNLPTVAKMNPANLAVFVFDNQAYS
                     GTKISEPTATAGKTDLAGMARSAGIESAITVRDVVGFKSKAAGAIGVAGLRFVVAKVE
                     ESRGHREIERTTVDLLENKYRFVRYLERTEGRAIFRGRG"
     gene            complement(4110..5069)
                     /locus_tag="VE170_17100"
     CDS             complement(4110..5069)
                     /locus_tag="VE170_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17213.1"
                     /translation="MRYVIAVAMISLSPFISSAQAQKISIAVSNPDMSFLSGGVAKFK
                     GFFKEEGLDAELVQISANVSIAALAGGNVDYNLILQSVVTGNLRGLPIKVAGILIERP
                     NHVVVVNPKITRFADLKGKKIAVSSFGSATDILARLTVEHFKLNPSKDVQFIAAGGGS
                     GRLAQLESGLVDAAVVAPPSDAIAESRGFKSLVRTRDILIFPVNGVGLHEQKLKNHRG
                     EVKRVLRALLRANRFILDDKKGAIDILQRWGRTSLAVAEQGYLSSSANFSRNLLAPRD
                     ALEKVIYSTKLNIDLKRDVSLDDVFDFSIVREILREMGETPRD"
     gene            complement(5129..5635)
                     /locus_tag="VE170_17105"
     CDS             complement(5129..5635)
                     /locus_tag="VE170_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17214.1"
                     /translation="MTPEAANKVVAGLKQAGIDFVAYLPETRLSEILPVLKADKSFML
                     VPVASEAEAVGIAAGASLGGKQPALYMEGTGLFVSSYNLLTLGERYGVPMLLLISYIG
                     GMDDRRSTFLYSHYGTHMVPQLEALGIQYEIVNQAANIETRIENAVRLMHGLKQPVAL
                     LFTGEFTI"
     gene            complement(5648..6538)
                     /locus_tag="VE170_17110"
     CDS             complement(5648..6538)
                     /locus_tag="VE170_17110"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ17215.1"
                     /translation="MIRGVLLPIITPFDDKVRVDEQMMRQLVDFHIKAGVQGLFVLGS
                     TGQGPAMTTEERKQTAAIALDQAKKRVPVVIHVGTADAGSAVELAEHAAAHGADAVAI
                     VPPYYYSDHTEFEIMAHYKAVHKAMPLPIYIYENPKYSGISIPPGFAVRMKEQVPALQ
                     GIKVAYGQGALLEYVRLMPDVSVFTGNADLFGLVPFGIAGMINPPTSFAPEICVDLFK
                     KLDSKDYVGAVEAQKKVDTAARIVAARLRKWGRVPLQEVYRMRGFAVKRFPKWETEQM
                     PKDEIVKLERELRDNGILGQ"
     gene            complement(6707..>7789)
                     /locus_tag="VE170_17115"
     CDS             complement(6707..>7789)
                     /locus_tag="VE170_17115"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ17216.1"
                     /translation="KVLGEELVLFRDQNGQVGLLGEHCPHRGTSLEYGDIEDGGLRCP
                     YHGWLFDVHGRCLEMPAEPKDSKFCEKVKQLSYPVRELGGLIFAYMGPEQANPPPLPQ
                     YAPLLERGGQRQVEPVRHADYNWFNFFENSADPAHVCVLHRHAGYGQQSWGDQFFSYT
                     NMPDFEFVEMDYGMKVVMTKPGPRPGTEFVDEMSLALPSIVQVGDTEFVHAKMEAAAL
                     INEGSRCEHWMFVTPNDDASFMLFTADNYLGPVENFFDKLREMRAKEAPIQEVKPYDK
                     RKYMPYKGNVRKEDIMTQGTQGLLGERREQLGAADRGVIKFRKIVLDAIETALAGQQP
                     KGAPAGNDAAAVVHLDTRVGVREKVN"
BASE COUNT         1630 a         2386 c         2082 g         1691 t
ORIGIN      
        1 gcggtcgact gcgccgcttc gagcggatcg agcagcgcgt ccaactgttc ataatcaacg
       61 tcgatcaatg cgatcgccgc ctcggcgatt tcctctgtct ctgccgcgac caccgcgact
      121 ttctcgccga tgaagcgcac cacctcgtcg gcaagcaccg gcatgtcgta aattttgcgc
      181 ccgatgagtt ggcctttgac atcggcgccg gtaaccacgc cgtgaacgcc cggcagcgcc
      241 agcgccttgc tgacatcgat tttgtcaatc cgaccgtaag cgattgggct gcgcagcgct
      301 ttcgcccaga gcatgttggg aaggactatg tcggtggcat aaaccgccgc gccggaaact
      361 ttttcccggc cttcggatct cgtggtagaa acgccgatga tcttttgctg cgcgctactc
      421 tttacctgca ctggactcac ggctggtctg actccgctat tttgctcttg tcagcggctt
      481 cgaaagccgc ctgtagacaa acgccatgca gatcgacagc acacaggccg caatattcca
      541 aagtataacc cggccgcaca tgcgccaagc cgtcttcaat aagggtcctt aacaccaggc
      601 ggcgaaaact gccgtgaccc atgaccccgt cgtgttcttt caggtcggcc tcttcatggg
      661 tcgagaaata gacggcctgc tcacgagaaa ggccgtagtg agccgtaagc gccttataaa
      721 aatccgccga ccagtagccc gtctgttctt cggtggcgcc ggcagcgtag aactccgcca
      781 tcgtcccctc ccaaagcagc gagcgcttga agtcgagttt ggcgcgaaac tcgggcagca
      841 tcggcgccat gaagatttca tcttcgctaa tgcccagcgc tttcgcggtc tccaccacca
      901 cgtgcacatg gccgggcggc ttcggatgta tcaactcgtc ggccagcttc tcggccatgc
      961 gcgccatcag atcgcgatgt ttgcgaaaaa acgaaatatg cttgtgataa gaagccgctt
     1021 ccacggtgtt gatctcgatg gtgtaagaac cccagttctt gaaaaacgtg cgcaacgtct
     1081 ccatcggcaa ggtgccggct cgtaattgcc gcatgaactg cgattcggtg actcgggtgc
     1141 gccagcgcgc ggccacttta cgataatgtt cgtcgacata tgcggcgccc tgttcagtgc
     1201 caatgctttc actcataagc tccagctctt ttgcgtttaa gagaattatc cgcgaccgcg
     1261 gaaaatcgct cggccttcgg tgcgttcgag atagcggacg aactgatatt tgttttcgat
     1321 cggatcaagg tcggtgcgca gaatctcccg gtgcgtcacg ctctcctcca ctttgcagac
     1381 gataaatcta agctgattct tcgcaacggc gtcatcgagt tccttttgga atccgtcgag
     1441 atcacggacg gtggttgcct gttcgatgcc ggcgccacgc gccatcatcg ccaagtcggt
     1501 tttgcccgcc gtcgccgtcg gttcgctgat gcgcgtaccg ctgtaaactt ggttatcgaa
     1561 gaccagcacg ataagatttt gcggattgag attggccaaa gtcggcaggt taaagagcga
     1621 cagcagcaca ctgccgtccg attcgatgac gatgactctc cgattaggca agccgatcgc
     1681 cactcccatg ccgacgccga gagcggtccc catggttccg accaacagat tgccgtggtg
     1741 ctgtgacaga ttggcccact cgattttttg cccgctgagg gacgtcacca ccagctcgtc
     1801 ggtaatccgc ggtgcgagca gcttcagaca atcatagcga atcatcgggt gaaatctccg
     1861 gtaaacacca gcgcgacggg cagcttgagc gcgtgcatgt ggcgaacagc gtcttgaata
     1921 tcgtcttcaa gcctatcgcc gttgacgaga atctgcgttt taatctgcag ggcgtcgagc
     1981 aatggttcga cataagttcc ggacaccgcg taaacggcat tgttgcgctg atcgttcatg
     2041 ctgccgagat acgcgacgac cagcagcaag ggcacgccat agcgcctggc gatcgaaacc
     2101 agcgtataac aggagacgta gagtccggta cactccatgt agatcgccac ctgcttgccc
     2161 gtcagcgccg cgcccgcgcc gatggtcacc gcctccgatt cgctggccac cggcaccagc
     2221 gtgaaactcg ggtcgctgcg cagcaacggc aggatctgac taagccgggt ttccggcaga
     2281 taggtgacca gatcgatgcc ggcttgcttc aaaccagcga ccaatcgttt agctaatgcc
     2341 gcttccatta tggcacctgg agcggtccgt tcgtcgacgc tcggggaaaa atcatttgaa
     2401 ccccaattcc tgattcactt cacgctgcaa cgagaagtcg aaaatcttcg ccgcagcgac
     2461 aggctctttt agtttcaaca gctcggcatc ggctttgaga aattcgtcga cctccttggc
     2521 gctggcgatg ccgtcagtgg tgaaggccgg tttggccaac cgataggatt cccgcgccac
     2581 ttcgaaatcg acactttgaa atttagccaa aacctcactt gtcccgcgct cgttttctag
     2641 aaaataacga tgggcttttg cccgggcgcg aagaattcgt cgcaacaatg gcctgttctc
     2701 acgcagcagc ttttccgtaa cggcggtgcc gttctgaaga ttgaaaagat cttcgagagc
     2761 ggagccgaga acattcatat tatatttatc gcgcgccaga atcaccgtcg gcggcgacag
     2821 cgccgcggca tctatgacac cgctaaccag gctttgcatt tgcgccggaa cgtcgccgcc
     2881 gacaaccacc gtaatatcct tatccggatc gaggccggct ttgcggatca gacgggtcgc
     2941 tgcggtgtgc tgcgaaccac cgaaggtcgt cgttcccaac ttcttgttct tcagctgagc
     3001 tatcgaagtg atttccttcc gggagaccag ccagaaaaga ttacgcttga ggctcacgct
     3061 cagaactttc aagggaatct cgctccgttc gagagcgcga atcacggtgc caacgccgtt
     3121 ggtggcttga acctggccgc ttagcgccgc cgcgatcgcc acgttgccgc ggatctgcac
     3181 gatatcgacg tcgagccctt cgtcccggaa aaagccgcgc tccttggcga catagagatc
     3241 gatggcggcg atgctccggc tgggaatcga aaacacgatc ttctccagcg caccctgggc
     3301 gaaagccttc tgcgccagac ccagccctag cgcggtgagg acgagccaga taactgtcgg
     3361 ggtaagaagt agtcgtcgca agttcattcg gttcacaacc ttttgggtaa acggaaatca
     3421 accatgcacg cgaatcgagt gatctgtcgg cagcccgcgt cgatcatttc gattccagcg
     3481 agcaaatatt gcaccgaccc cgatcatccg cggccgcgaa atatcgcccg gccttcggtg
     3541 cgctccagat aacggacaaa ccgatattta ttttccagca ggtcgacggt ggttctctcg
     3601 atctcgcggt gaccgcggct ctcttccacc ttggcgacga caaatctgag cccggctacg
     3661 ccgatggctc cggctgcttt actcttgaag ccaacgacat cgcgcacggt gatcgcgctt
     3721 tcgatgcccg cgctgcgcgc cattcccgcc aagtcggtct tgcccgccgt cgccgtgggt
     3781 tcactaattt tggtcccgct gtaagcctga ttatcgaata cgaagacggc aagattcgcc
     3841 gggttcatct tcgccaccgt cggtaagttg aacagcgaca gcagcaagct gccgtcggat
     3901 tcgagcacga tgacttttct attaggcagg gccagcgcca gccctaatcc caccgcaatc
     3961 gcgttgccca tcgaccccag cagtaaattc ccttcgtgct tggacaaatg gccccattca
     4021 acccgctggc cgctcaacga tgtcaccaca agttggtcgg tgattaacgg agctaactgc
     4081 tcgagacatt cgtaacgctg catggtggcc taatctctgg gcgtctcgcc catttctctg
     4141 agaatttccc ggacaatgct gaaatcgaat acgtcgtcca acgacacgtc gcgtttgaga
     4201 tcgatattga gcttggtgct gtaaatgact ttctccaagg catcccgcgg cgccagcaaa
     4261 ttgcggctaa aatttgccga ggaggataag tacccctgct ccgccaccgc caaggatgtg
     4321 cgtccccaac gctggagaat atctatggcg ccctttttgt cgtcgagaat gaatcggttg
     4381 gctctgagca acgcgcgcaa cacgcgcttc acttcgccgc gatgattctt cagcttctgt
     4441 tcgtgcaggc cgacgccatt gacggggaag atcagaatat cgcgagtccg caccaaactc
     4501 ttgaaaccgc gcgactcggc gatcgcatcc gacggcggcg ccaccaccgc ggcgtcgacc
     4561 aggccggatt ccaattgcgc cagccgtccg gaaccccctc ctgcggcgat gaactggaca
     4621 tctttactgg gattgagctt gaaatgttcc accgtcaggc gcgccaagat atccgtcgcc
     4681 gagccgaagg aactcaccgc gatttttttc cctttgagat cggcaaaccg ggtgattttc
     4741 ggattgacga cgacgacgtg attgggccgt tcgatcaaaa tccccgcgac cttgatcggc
     4801 aatccgcgca gattacccgt gaccacggat tgcagaatca gattgtaatc gacattgccg
     4861 ccggccagcg cggcgatcga tacgttggcg ctgatttgca ccagttcggc atccaaccct
     4921 tcttctttga aaaagccctt gaacttggcc acgccgccgc tgagaaacga catatccggg
     4981 ttggagacgg cgatgctgat cttttgcgcc tgcgccgaag agataaacgg cgacaaggaa
     5041 atcatcgcaa ccgcgatcac gtatcgcatc ttgtgcagtc tcgtcatttc attttgcccc
     5101 tgactcctta cctttcaccc attcgtcatc aaatcgtaaa ctcaccggta aacagcagcg
     5161 ccaccggctg cttcagccca tgcatcagcc gcaccgcgtt ctcgatacga gtctcaatgt
     5221 tcgcggcttg gttgacgatt tcgtactgga tgcccaacgc ctcgagctgc ggcaccatat
     5281 gcgtgccgta gtgcgagtaa agaaacgtgc tgcgccggtc gtccatgccg ccgatataac
     5341 tgatcagcaa cagcatcggc acgccgtaac gctcaccaag ggtaagtaaa ttgtaggacg
     5401 agacaaacag gccggtgcct tccatgtaca gcgccggctg cttgccgccg agggaagcgc
     5461 ccgcggcgat gccgaccgct tccgcttcgc tggccacggg cacgagcatg aacgatttgt
     5521 cagctttcag caccggcagg atttcgctta gccgcgtctc ggggagataa gcgacgaagt
     5581 cgatgcccgc ttgcttgagc ccggcgacaa ctttattggc ggcttcaggc gtcaaaggct
     5641 cctcgcttca ctgcccgaga ataccgttgt cgcgcagctc gcgctccaat ttgacgattt
     5701 cgtccttggg catctgctcg gtttcccact tgggaaaacg tttcaccgca aaaccacgca
     5761 tacgatagac ctcctgcagc ggcacccggc cccatttgcg caagcgcgcc gcgacgatac
     5821 gagccgccgt atcgaccttc ttctgcgcct cgaccgcgcc gacgtagtcc ttgctatcga
     5881 gtttcttgaa taagtcgacg cagatttccg gagcgaaact ggtcggtggg ttgatcatcc
     5941 cggcgatgcc aaaaggcacc aggccaaaca aatcggcatt gccggtgaaa accgaaacat
     6001 ccggcatcaa gcgcacgtat tcgagtaacg cgccttggcc gtacgcgact ttgatgccct
     6061 gcaatgccgg cacctgctct ttcatgcgca cggcgaagcc cggcgggatc gagatgccgg
     6121 agtatttcgg attctcatag atataaatcg gcagcggcat cgctttatgc accgctttgt
     6181 aatgcgccat gatctcgaac tcggtgtgat cggaataata atagggcggc acgatcgcca
     6241 ccgcatcggc gccgtgagcc gcggcatgct cggccaactc cacggcgctg ccggcgtcgg
     6301 cggtgccgac gtgaatcacc accggcacgc gcttcttggc ttgatccaac gcgatcgccg
     6361 cggtttgctt gcgctcctcg gtggtcatgg ccgggccctg acccgtggag ccaagcacga
     6421 acagtccctg gactcctgcc ttgatatgaa agtcgaccag ctggcgcatc atctgctcat
     6481 cgacacgaac cttgtcatcg aacggcgtga tgatcggtaa caacactcca cgaatcatga
     6541 tttcctccta gtgcaaccag gcaacttgca gcgacaccac tatcacactt attgaaacta
     6601 tatccccgac gatcttctac ctgaggggaa agtatcggac gtcacgaata accccatgct
     6661 atagctgcac agcggtgcaa ttttttaatt ttgaatttag taatttttaa ttgacctttt
     6721 cccgcacccc cactcgagta tccaaatgca ccaccgcggc ggcgtcattg ccggccggcg
     6781 cgcccttcgg ctgttggccc gccaacgcgg tttcgatggc gtccaagacg atcttgcgaa
     6841 atttgataac cccgcgatcc gccgcgccca gctgttctcg acgctcgccc aagagcccct
     6901 gagttccctg cgtcattata tcttccttgc gcacattgcc tttgtaaggc atgtatttgc
     6961 gcttgtcgta gggcttcact tcctggatcg gcgcttcttt agcgcgcatc tcgcgcaact
     7021 tgtcgaagaa attttccacc ggaccgaggt aattatcggc ggtaaacagc ataaagctgg
     7081 cgtcgtcatt gggcgtgacg aacatccagt gctcgcagcg cgagccttcg ttgatcaacg
     7141 ccgccgcttc cattttggca tggacgaact cggtgtcgcc cacctgcacg atactcggca
     7201 gcgccaagct catctcgtcg acgaattccg ttcccggccg cggcccgggc ttggtcatca
     7261 ccactttcat gccgtagtcc atctcgacga attcgaagtc cggcatattg gtgtaactaa
     7321 aaaattggtc gccccaggac tgctggccgt agccggcgtg gcgatgcagt acgcagacat
     7381 gggccgggtc ggcggaattt tcgaaaaaat tgaaccaatt gtaatcggca tggcgcaccg
     7441 gctcgacctg acgctggccg ccgcgttcga gcagcggcgc gtattgcggc agcggcggcg
     7501 ggttcgcttg ttccggcccc atgtaggcga agatcagccc gcccagctcg cgcaccggat
     7561 aggacagctg cttaaccttc tcgcagaatt tgctgtcctt gggttccgcc ggcatttcca
     7621 ggcagcggcc gtgcacgtcg aacagccagc catgataggg gcaacgcagg ccgccatctt
     7681 cgatatcgcc gtactcaagg gatgtccccc ggtgcggaca atgctcgccg agcagaccga
     7741 cttggccgtt ctgatcgcga aacaacacca actcctcgcc gagaacttt
//
LOCUS       DASQKS010000660         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1635969, whole genome shotgun sequence.
ACCESSION   DASQKS010000660 DASQKS010000000
VERSION     DASQKS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635969"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VE170_17120"
     CDS             complement(<1..631)
                     /locus_tag="VE170_17120"
                     /inference="COORDINATES: protein motif:HMM:NF026192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17217.1"
                     /translation="MIENMADDGRAKKKPTLTGPEKAAIFLALVGEELAGAIAGELQE
                     KEIVMLRQGVHRMAQIETEHIDEVFEETCKILKKANIFAEGTTDYLQRVLTKAMGAEK
                     AATVLSRIFTEEGDESYGIDSLHEMDAKVLGQFLQEEHPQTIAFVLAHLYPAHGGEVF
                     GTLSEDKQAEVAYRITTLGSTSPGAIEEISKVLRNAVRQVQGKQVGGVRP"
     gene            complement(628..2259)
                     /gene="fliF"
                     /locus_tag="VE170_17125"
     CDS             complement(628..2259)
                     /gene="fliF"
                     /locus_tag="VE170_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HYJ17218.1"
                     /translation="MPEQLEKFLSDLKQILARLLAFLTPQQKRLIFIGLPVFGALGYA
                     GMFAFEQMNYGPLYTNLAPSDGSAVVKELEAAKIPYAITNGGTVIEVPRGVLYQTRLK
                     LAGKGIPAGGGVGFEIFEKNQFGASEFTQQVNYLRAQQGELSRTINSITAVQSSRIHL
                     AIPSRTNFLGPEEKPSASVVVELRPGYQLSPDQVQGIINLVAGSVPKLSSDKVTVIDG
                     SGRPLKEIVVAVPSTEAEKVNALKLKHEAELQRRVETMLDPVLGSGKVIVRANVQISL
                     QETLMTKEEFDPDNKVVRSQRQSSDDSGAKGGNPGVQANVPGGDATKATDGGAKRSSE
                     MVTYEVGRTTSRIMEPRGQIQKLSVAVMVDGKYENEKYLARSAEEMEVIKGIVKRAVG
                     FDTERGDEIEIANVPFKIQPASPFNPLVAPTLKDMAQTPMGMAVAGGALLVLGTLAFF
                     MLRRKKPAASAAEPAAAIVALPTEPSTPAAAIQEEVAAAAQKIIVAEDPRKEQLAQIA
                     HDYHDASVRIIRTWLQEDAAKARAAAASGGIIANP"
     gene            complement(2431..2730)
                     /gene="fliE"
                     /locus_tag="VE170_17130"
     CDS             complement(2431..2730)
                     /gene="fliE"
                     /locus_tag="VE170_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HYJ17219.1"
                     /translation="MSIVKNVDLVYRPAPLVPSKNLDEPQKGDFGNVLKSAMGEVGDL
                     QEKANQSIQQMVGEGKGDLQETMIALEKADVSFKLMMQIRNKVLDAYQEIMRMQV"
     gene            complement(2765..3214)
                     /gene="flgC"
                     /locus_tag="VE170_17135"
     CDS             complement(2765..3214)
                     /gene="flgC"
                     /locus_tag="VE170_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HYJ17220.1"
                     /translation="MDLFKIFSISGSGMAAQKSRMSVVAGNLANSESTRTVDGGPYRR
                     RDVIFQAAPPPGEFADELNDIAADQERAQGVEVVGVKQSNRPPRRIFDPSHPDASPEG
                     YVAMPDINSIEEMVDMLSAVRSYEANLSAFNSTKALIRKILDLGRTQ"
     gene            complement(3217..3645)
                     /gene="flgB"
                     /locus_tag="VE170_17140"
     CDS             complement(3217..3645)
                     /gene="flgB"
                     /locus_tag="VE170_17140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HYJ17221.1"
                     /translation="MAGLSLFKSTHQLLELSLRGRAARHEVLSANIANVDTPGYRPRD
                     LDFNAAMRAAAESSAPVTGEIPQRSNASASSGLDLQSAIYEPEYPDSRHGEDRLDGNA
                     VSLDRQMALLTENSLAYETSLTLLSRAMAALRYAIGEGRR"
     gene            complement(3647..5029)
                     /locus_tag="VE170_17145"
     CDS             complement(3647..5029)
                     /locus_tag="VE170_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ17222.1"
                     /translation="MLKRIAIVDDDPTMRVAISEALGGLGHTIEIFANGDAAQAHLLR
                     EGADLVISDVRMPGLTGLELLKRFHQLRFVLISGFATVPQAVDAMKLGAYDFLVKPFS
                     HRELVSLVETALACKTENPSVRAADEFVTTNPRMQGLLEFARQVAKSHASILIQGESG
                     TGKELLARYLHSHSRRHAGPFVAVNCAALPEGLLESELFGYERGAFTGAVTGKAGKFE
                     LAHNGTLLLDEISEMPLALQAKLLRVLQEREVDRLGGKKPLQIDIRVISTTNRELREM
                     IRAGSFREDLFYRINVVPTRIFPLRERLDDIAPLATAFFAKRGCPGLSLAPSTLARLH
                     ENPWRGNVRELFNVLERSAIIAEDGVILPEHLLLEDELPSWSRSAAPAAVLPDTHTIA
                     ENTLSRASQEYAGSRQRSVREMEEQLIFQTLEQANNNRTQAAKLLGISVRTLRNKLKC
                     YRERIQLVEA"
     gene            complement(5023..>6029)
                     /locus_tag="VE170_17150"
     CDS             complement(5023..>6029)
                     /locus_tag="VE170_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ17223.1"
                     /translation="AVLDHELHPTFVNRRLTELAGEVNDQRIRQWLGDKLVTSLQGGD
                     NCFLPFECEKVVQGPDGAMVPVHFSVAEVATGDAASHYVLVFQDVTTLKRMEAEAART
                     RRLASLGMMASELAHQVKNPLGGIELCASLLKEKSAGDSQRLAGEILAATRRLSTTVS
                     KLLAFAAEPTIVSDVLTVATLMKDVLQECVALFDDPAWSIVVSIERDLPPVWGDRSLL
                     VQALVNLIVNAKEAMPNGGRVCLKAGLSPFSTLNGQIHKAVEIKVRDHGVGIAAENRE
                     HIFDPFFSTKSQGTGLGLAFAHKIISAHRGSIEITSAPQAGSEFTIGLAAAEEIPAAC
                     "
BASE COUNT         1168 a         1790 c         1737 g         1334 t
ORIGIN      
        1 ccggacgcac tccaccgacc tgcttaccct ggacctgacg cacggcattg cgcagcactt
       61 tgctgatctc ctcgatggcg cccggagagg tgctaccaag ggtggtaatg cgataggcga
      121 cctcagcctg tttgtcttcc gaaagcgtac cgaagacctc gccgccatgg gccgggtaga
      181 ggtgggctag gacaaacgcg atggtctgcg gatgctcctc ctgaagaaac tggccgagga
      241 ctttagcgtc catctcatgc agcgaatcga tgccgtagga ttcgtcgcct tcctcggtga
      301 agatacggct caataccgtc gcggctttct ccgctcccat cgctttggtc aatacccgtt
      361 gcagataatc ggtcgtgcct tcggcaaaga tattggcctt tttgagaatc ttgcaggtct
      421 cttcgaaaac ctcgtcgata tgctcggttt cgatctgcgc catgcgatgg acgccctggc
      481 gcagcatgac gatctctttt tcctgcaatt cgccggcgat cgcgccggca agttcttcgc
      541 cgaccaaggc caagaaaatc gccgccttct cggggccggt caaagtcggt ttcttttttg
      601 ctctgccgtc gtcagccata ttttcaatca agggttggca ataatgccgc cgctagctgc
      661 ggccgcacgg gccttcgccg cgtcttcttg taaccaggtt cgaatgattc gtaccgaggc
      721 gtcatggtag tcatgggcga tctgcgccaa ctgctctttg cgcgggtctt ccgccacgat
      781 gatcttttgc gccgccgccg ccacttcctc ctgtatcgcg gccgcagggg tagacggttc
      841 cgtcggcagc gcgactatcg ccgccgccgg ctcggcggcg ctcgccgccg gtttcttgcg
      901 gcggagcata aagaaggcca aggtgcccaa gaccaagagt gccccgccgg caacggccat
      961 gcccattggg gtttgcgcca tatccttcag ggtcggggcg accagcggat tgaacgggct
     1021 cgccggctgg atcttaaacg gcacattggc gatttcgatc tcgtcgccgc gttcggtgtc
     1081 aaagccgacg gcgcgcttga cgatgccttt gatgacttcc atctcttcgg cgctgcgcgc
     1141 aagatacttt tcattttcat atttgccgtc gaccatgacg gccaccgaca gcttttgaat
     1201 ctggccgcgc ggctccatga tccggctggt ggtgcgcccg acctcatagg tgaccatctc
     1261 gctgctgcgt ttcgcgccgc cgtcagtggc tttggtggca tcgccgcccg gcacgttagc
     1321 ctgcactccg ggattgccgc ctttggcgcc actgtcgtcg ctcgattggc gctggctgcg
     1381 cacgacttta ttgtccggat cgaactcctc tttagtcatc agggtttcct gaagactgat
     1441 ctggacattg gcgcggacaa tcactttgcc ggagccgagc accgggtcga gcatagtttc
     1501 gacccgccgt tgcaattcgg cttcatgctt gagcttgagc gcattgactt tctccgcctc
     1561 ggtgctgggc acggccacga cgatttcttt gagcggacgc cccgagccat cgatgaccgt
     1621 gaccttgtcg ctgctgagct taggcaccga accggcgacc aaattgataa tgccctgaac
     1681 ctgatcggga ctgagttggt aacccggccg cagttcgacg accaccgacg ccgacggttt
     1741 ctcttccgga ccgagaaaat ttgtgcgcga cggaatcgcc aggtggatcc ggctcgactg
     1801 caccgcggtg atcgaattga tcgttcgcga tagttcgcct tgctgggcgc gcaagtaatt
     1861 cacctgctgg gtgaactcgc tggcgccaaa ctggtttttt tcaaagatct caaagccaac
     1921 gccgccaccg gccggaatgc ctttgccggc gagcttcaaa cgcgtctggt acagcacgcc
     1981 gcgcggcact tcgatgactg tgccaccatt ggtgatcgcg tatgggattt tcgcggcctc
     2041 gagttccttg accacggcgg aaccgtccga tggcgcaaga ttggtgtaga gcggaccgta
     2101 attcatctgc tcgaaggcga acatgccggc gtagccgagc gcgccgaaaa ccggtagacc
     2161 gatgaagatc agccgtttct gctgcggcgt cagaaacgcc agcagccggg ccaggatctg
     2221 tttaaggtcg ctaagaaatt tttctagttg ttcaggcacg aaacatctca cccgtcataa
     2281 ataccaactg aatttcaaac ccctcgtctc gaatcgttcg cgggcaaaaa taaacttcga
     2341 gatccgctag gcaacaggtt tagaatcttc tcgttttagt ttatttgaac attccggaat
     2401 gccgattctc ctttagtttg cggcattgaa tcacacctgc atgcgcatga tctcctggta
     2461 ggcgtcgagg actttattgc ggatttgcat catcagtttg aaggacacgt ccgccttctc
     2521 gagagcgatc atggtttcct ggagatcgcc cttgccctcg ccgaccattt gttgaatcga
     2581 ctggttggct ttctcctgca agtcgccgac ttcgcccatg gcgctcttga gcacattgcc
     2641 gaaatcgccc ttctgcggct catcgagatt cttgctcggg acgaggggcg ccggacgata
     2701 aactagatcg acgtttttga caatactcat ggtgaaaatt gctccttaaa caaagacttc
     2761 gcgattactg ggtccggccg agatccagga tttttcgaat caaggctttg gtgctgttga
     2821 atgccgacag attggcttcg taagagcgca cggctgaaag catgtcgacc atttcttcga
     2881 ttgaattgat atccggcatc gcgacataac cctcggggct ggcatcggga tgactgggat
     2941 cgaagattcg gcgcggcggt cgattgcttt gcttgacacc gactacctcc acgccttggg
     3001 cgcgctcttg atcggcggcg atgtcattga gttcgtcggc aaattcaccc ggaggcggcg
     3061 ccgcctgaaa aatgacgtcg cggcggcggt acgggccgcc gtcgacggtc cgcgtcgatt
     3121 cgctattggc caagttgccg gcaaccacgc tcatccggct tttttgagca gccatgcccg
     3181 atccgctgat ggagaatatt ttgaacagat ccataattac cttcttcctt cgccgatggc
     3241 gtagcgtagc gccgccatcg cccgcgataa cagagttagg gaggtttcgt aggccaatga
     3301 gttttcggtg agcaacgcca tttgccggtc gaggctgacg gcgttgccgt cgagccggtc
     3361 ctcgccgtgg cggctatcgg gatattccgg ctcgtaaatc gccgactgga gatcaagccc
     3421 ggagctggcc gatgcattgc tgcgctgggg aatttcgccg gtgactgggg cactgctctc
     3481 cgcggcggcg cgcatcgcgg cgttgaagtc caagtcgcgc ggccggtaac cgggagtatc
     3541 gacgttggcg atattggcac tgagcacttc atggcgtgcc gcccgcccgc gcagagacaa
     3601 ctccagtaac tggtgcgtcg acttgaaaag acttagtccg gccatttcat gcctccacca
     3661 attggattcg ttcgcgataa cacttcaatt tgttacgcag cgtgcgcacg cttataccca
     3721 agagcttggc cgcttgcgtg cggttattat tcgcttgctc gagagtttga aaaatcagtt
     3781 gttcttccat ctcgcgcacc gagcgttgcc ggctgccggc gtattcctgg ctcgcccgtg
     3841 acagcgtatt ctcggcaatc gtgtgggtat caggcagcac tgccgccggc gcggcactcc
     3901 ggctccacga cggcagttca tcttcgagta acaagtgctc tggcaagatc actccatctt
     3961 cggcgatgat cgcggaccgt tcgaggacgt taaacagctc gcgcacgttg ccgcgccagg
     4021 gattctcgtg taaccgcgcc aaagtcgacg gcgccaagga caagcccgga cagccgcgct
     4081 tggcaaaaaa tgccgttgcc agcggtgcga tgtcgtcgag ccgttcgcgc agcggaaaaa
     4141 tccgcgtggg cacgacattg atgcgataaa acaagtcctc gcgaaaactg ccggcgcgaa
     4201 tcatttcgcg gagctcccga ttggtcgtgg atatcacccg aatatcgatc tgcagcggct
     4261 tcttgccgcc taatcggtcg acttcccgtt cctgcagcac gcgcaagagc tttgcctgca
     4321 gcgcaagcgg catttcgctg atctcatcga gcagcagagt gccgttgtgg gcgagttcaa
     4381 attttcccgc tttgccggtt accgcgccgg taaatgcgcc gcgctcataa ccgaacagct
     4441 cggactccaa cagcccctcg ggcaacgccg cgcaattgac cgcgacgaag gggcctgcat
     4501 ggcgccggct atgactgtgc agatagcgcg ccaggagttc cttgcccgtg ccgctttcgc
     4561 cttggattag gatcgaagcg tgggacttgg ctacctggcg ggcaaattcg agcaagccct
     4621 gcatgcgcgg attggtggtg acgaactcgt cggcagcgcg gacactcggg ttttcggttt
     4681 tgcacgccaa ggccgtttca actagggaaa ccagttcacg gtgggaaaac ggcttgacca
     4741 ggaaatcgta agcgcccagt ttcatggcat ccacggcttg cggcacggtg gcaaaaccgc
     4801 tgatgagaac gaatcgcagc tgatggaagc gcttcaataa ctcgaggccg gtcaatcccg
     4861 gcatgcgcac gtcgctgatc acgagatcgg cgccttcgcg gagcaagtgt gcctgggcgg
     4921 catccccatt ggcaaaaatt tctatggtat gcccaagccc gccgagcgct tcgctgattg
     4981 cgacgcgcat ggtcgggtcg tcatcgacaa tggcgatacg ctttaacatg cggcaggaat
     5041 ctcctcggcg gctgccaaac cgatggtaaa ttcactgccc gcctggggcg ctgaagtgat
     5101 ttcgatggag ccgcggtggg cgctgattat cttatgggca aatgccaagc ccaggccggt
     5161 gccctggctc ttggtgctga aaaacggatc gaaaatatgc tcgcgatttt ccgccgcgat
     5221 gccgacgccg tgatcgcgca ctttgatttc aacagcctta tgaatctgac cgttgagcgt
     5281 cgaaaacggt gacagccctg ccttcaagca gacccggccg ccgttgggca tcgcttcctt
     5341 ggcgttgacg atcaggttga ccaacgcttg cacaagcaga ctgcgatcgc cccaaaccgg
     5401 cggcagatcg cgctcgattg aaacaacgat ggaccacgcc ggatcgtcga acagggcgac
     5461 gcactcttgc aagacatctt tcatcaaagt ggcgaccgtt aacacatccg acacaatcgt
     5521 gggctccgcg gcgaatgcta acagcttgga caccgtcgtc gacaaccgtc gcgtggcggc
     5581 gaggatttct ccagcgagcc gttgcgagtc gcccgccgac ttttccttca agagcgaggc
     5641 gcacagctca atgccgccga ggggattttt cacctgatgc gccagctcgg acgccatcat
     5701 gccgagggac gccaagcggc gcgtgcgcgc cgcctccgcc tccatacgct tcaacgtcgt
     5761 gacatcttga aaaaccaaga cataatgact tgccgcatcg ccggtggcga cctcggcgac
     5821 ggaaaaatgc acgggcacca tcgcaccatc cggtccctga actacttttt cgcattcgaa
     5881 aggcagaaag cagttgtcac cgccctggag gctagtgacc aatttgtcgc ccagccattg
     5941 gcggatgcgt tggtcgttga cctcgccggc gagctcggtc agacggcgat tgacgaaggt
     6001 tggatgcagc tcgtggtcga gcacagcga
//
LOCUS       DASQKS010000661         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_338631, whole genome shotgun sequence.
ACCESSION   DASQKS010000661 DASQKS010000000
VERSION     DASQKS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338631"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..648
                     /locus_tag="VE170_17155"
     CDS             199..648
                     /locus_tag="VE170_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17224.1"
                     /translation="MPKPFVVTPADAPSPLKIVGEQITVLAAGAQTGSYEIFLQRGPE
                     SSGPPPHNHPWDESFYVIKGEIAFGIDDSETTALPGTLVHLPAGTTHWFRFGKGGGEM
                     ISMTSREGASQMFTDFDREISPENPDLGKLVAVGQRHGLEVIPPTNR"
     gene            725..2248
                     /locus_tag="VE170_17160"
     CDS             725..2248
                     /locus_tag="VE170_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17225.1"
                     /translation="MLKFAIVALSVLFFQLTGPASSMAAAKTDQKVSAVVALPGVQVE
                     TLNPYGHSTTQIYPTWKHVIEPLVEWSWSKKRVVPILAESWSNPDSNTWLFKLKKGIK
                     FHDGSEFTSADVVHSLTRVQKDPDSKQVTSIAHVTAIEAVDPLTVRLRTKNPDAALLF
                     RLGQRFITNKAAYDRLGAAAADKLALGTGPYKFKEWVRGQWFVVEKNPGYAHSDHKPT
                     VDEIVFRNIPESEVAITSLLNKEVDIISNVPPESAARVTGLARIESARTINIMFLGMH
                     SSVPQFQNKLVRQAVNYAIDRKALTQNVLKGYAFPMDAPVGPDQYGYHREIRPKYTHD
                     PARAKKLLAQAGFPNGFEVDFLVPVGQYNKVKDVAEAIGGMLNDVGIRAKLRTQDQDS
                     GFAAIQNGKAGMYIFGRGAVVDPSEYLHQYFRTGVTKRLEFSNPAVDAALEAEQASFD
                     PAQRVKLLQKAMSVLMDEAPVVWLYQYQGLQGVSNKFDFKANPGEDVYGWDLKPRLR"
BASE COUNT          531 a          653 c          686 g          462 t
ORIGIN      
        1 ttcccagagc caagcgcgtg gccgtgcttc ccatccgagt tgaccttgac tccctgccac
       61 gttgtgagag atataatttt gcctttatta gaaaaggtgt gcggtgatac gcttgctcgc
      121 ggcgagaggt cgacattact ggtagcatca gacaaagtgc tacgcgcgaa tgcgctcaat
      181 taatcatgga ggttccctat gcctaaacct tttgtcgtca cgcccgcaga cgcaccgagc
      241 ccactaaaga ttgtcggcga acagatcacc gtgctcgccg ccggcgcgca aaccggcagc
      301 tatgaaatat tcctgcaacg gggacccgaa agcagcggac cgccgccgca taaccatccg
      361 tgggatgaat cgttctatgt cataaagggc gagatcgcat ttggcatcga cgatagcgag
      421 acgacggcct tgccaggcac cctcgtgcat ttacccgccg gcacgacgca ctggtttcgc
      481 ttcggcaaag gcggcggtga aatgatatcg atgacttcgc gtgaaggcgc gtcgcaaatg
      541 ttcaccgatt tcgatcgcga gatatcgccg gaaaatcccg atctcggcaa acttgtcgca
      601 gttggccagc gccacggatt agaagtaatc cctccgacta atagatagct tgcgctgctg
      661 ctgcagtttt cggttgagat tacaccgacc gcgacgactg aaaagatatg ggagtagacc
      721 tgccatgcta aaattcgcca ttgtggcttt gtctgtgttg tttttccaat tgaccgggcc
      781 ggcttcttca atggcggccg ccaagaccga tcaaaaggtt agtgccgtcg tcgccctccc
      841 aggcgtgcag gtggagacct tgaaccccta cggtcattcg acgacgcaga tctatccgac
      901 gtggaaacat gtgatcgagc cactggtcga gtggagctgg agcaagaaac gagtcgtgcc
      961 gattctcgcc gagtcgtgga gcaatccgga ctcgaacaca tggctgttca aactgaagaa
     1021 gggtatcaag ttccacgacg gcagcgagtt tacatcggcc gatgtcgtgc actctttgac
     1081 gcgggtgcag aaggaccccg atagcaaaca ggtcacctcg atcgcccatg ttaccgccat
     1141 cgaggcggtg gacccgttaa ccgtgcggct gcggacgaaa aatccggatg ccgcgctact
     1201 gtttcgcctc gggcaacgct ttattaccaa taaggcggct tatgatcgct tgggcgccgc
     1261 ggctgccgac aagctggcgc tgggcacggg gccttataag ttcaaggagt gggtgcgcgg
     1321 ccagtggttc gtcgtggaaa agaatccagg ctacgcccat agcgatcaca aaccgacggt
     1381 cgatgagatt gtttttcgca atattcccga atcggaagtc gccatcactt cgttgttgaa
     1441 caaggaagtc gacatcatct ccaacgtccc gcctgagagc gcggcgcgag tgacagggct
     1501 ggcgcggatc gagagcgcgc gcacgatcaa catcatgttt ctcggcatgc acagctcggt
     1561 gccgcagttc caaaacaaac tggtgcgcca ggcggttaac tacgccatcg accgcaaggc
     1621 gctgacgcaa aatgtgctca agggctatgc ttttccgatg gacgcgccgg tgggaccgga
     1681 ccagtatggc taccatcgcg agatccgtcc caagtacacc cacgacccgg cgagggcgaa
     1741 gaagttactg gcccaggccg gttttcccaa cggctttgag gtcgattttc tggtaccggt
     1801 agggcagtac aacaaggtca aggatgtcgc cgaggccatt ggcggtatgc tcaacgacgt
     1861 cgggatacgc gccaaactgc gcacgcaaga tcaagacagc ggctttgccg ccatccaaaa
     1921 cggcaaagcc ggcatgtaca ttttcgggcg cggcgcggtc gtcgatccca gtgaatatct
     1981 gcaccagtac tttcgcaccg gagtgaccaa gcggctggag ttctccaacc cggcggtgga
     2041 cgccgcattg gaagcggaac aggcgagctt cgatccggcc cagcgcgtaa agctcttgca
     2101 aaaggccatg tcggtgctga tggacgaagc gccggttgtc tggctgtacc agtatcaagg
     2161 cctgcaagga gtgagcaaca agttcgactt caaggcgaat ccgggcgaag acgtctacgg
     2221 ctgggatctc aagccacggc tgcgctaggg caggccaggc gaacgttcgc gcggcttgcc
     2281 gaccggaacg aaaattagcg aactaagcgt agcggcattg gcgtgatcaa cg
//
LOCUS       DASQKS010000662         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4546906, whole genome shotgun sequence.
ACCESSION   DASQKS010000662 DASQKS010000000
VERSION     DASQKS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546906"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1631
                     /locus_tag="VE170_17165"
     CDS             75..1631
                     /locus_tag="VE170_17165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HYJ17226.1"
                     /translation="MIIVTAAQMRELDRLTIEKYRTPSLTLMERAGTGIAAAIVQRFA
                     KAVKKGVLIVAGKGNNGGDGFVVARLLKKKRIPCEVALLARRDELTADAAHNLRAWIK
                     LKGKIIEIDGSKLELLSQRFAKHGLLVDAILGTGMKNEIAGLFGDVITLMNGSGLPTV
                     AVDIPSGLDSDTGQRLGVAIRADMTVALGYPKLGEVIHPGLTFVGELVVVDIGIDRRA
                     LVEVAPQIELLDRETVGWVVPRRQADTHKGTYGHVLVVAGARGKTGAAILSCRAAMRT
                     GAGLVTLAAPRALNVIFSGSLVEVMTEPLGEDAAEQLEMLSDADWRRLIERKNVLLFG
                     PGIGLSDATQSATRWLLRNLAMPWVIDADGLNNLALDLDRLRQAKTPPILTPHPGEMA
                     RLIGKSTADVNADRIEVARGFATAHRCHVILKGARTVIATAGGKVFLNPTGNPGMASG
                     GMGDVLAGIIAALLGQGLSAEDAMKLGVYLHGYVGDRVAEAKGPIGMIASDIVDGLPR
                     ALAELGSSAS"
     gene            2222..3250
                     /locus_tag="VE170_17170"
     CDS             2222..3250
                     /locus_tag="VE170_17170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ17227.1"
                     /translation="MKRFAWALLVVLASVTYGHAQTSFYQGKTIRIMVGNLAGDAYDL
                     WARIFAQHMGKYIAGHPNIIVQNMTGAGGVVAANYVYSVAKPDGLTLGTFGPSMYFDQ
                     LARRPKVRFDWAKFTWLGTPEQTEFILVMRTDTPYQSIEDVRKAAQPPKCGATGTSTS
                     GYYMPKFLDEGLGIKFNIVSGYPGGGEIDLGIERGELHCRNLTISTYFAREPFLSWHK
                     KGFVRPILQTGKKRDPLVADVPSIYELMDQYKTPEATRRVANVLLAPAVFGRPMVATP
                     GVPPDRVKILRDAYHQSLKDPALLEELKKRRWAVDAISGEELAKLAKEVVSQPPDVVD
                     RMMKLLGN"
     gene            complement(3496..>4022)
                     /locus_tag="VE170_17175"
     CDS             complement(3496..>4022)
                     /locus_tag="VE170_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17228.1"
                     /translation="MGLKEPKFATGYTGPELDVALLRGEIDARSNLATSILMRNSDWL
                     DKGTMNFHSIVEIPKGSKHPRFAKLAELESFAKSDSDRRLVTVFRSFRNAGAPFVVPP
                     GTPKDRVEILREAMRRAFKDPEFLADYKKIIKEDFEPVMPEEVDQAIREIPRDSETNE
                     LVKKLSGAGPMPVR"
BASE COUNT          825 a         1149 c         1191 g          857 t
ORIGIN      
        1 caaggttcaa gggttcaaaa gttcaagggc tcgaaccgga aacctaaaca gtcttaactc
       61 cgaaaatcgt aaccatgatc attgtcaccg ctgcacagat gcgcgagctg gatcgtttga
      121 cgatcgagaa ataccggacg ccgagcttga ccttgatgga gcgcgccggc accggtatcg
      181 cagcggcgat tgttcagcgc tttgccaaag ccgtcaagaa aggcgtgttg atcgttgccg
      241 gcaagggcaa caacggcggc gatggctttg tcgtcgcgcg gctgttgaaa aaaaaacgca
      301 tcccttgcga ggtcgcgctg ctggcgcggc gcgatgaact gaccgccgac gcggcgcata
      361 atttgcgcgc ctggatcaaa ctcaaaggca agattatcga gatcgacggt agcaagctgg
      421 aactgctcag ccaaaggttc gccaagcacg gccttctggt cgacgcgatt ctcggcaccg
      481 gcatgaaaaa cgaaatcgcg ggtctgttcg gcgacgtgat cacactgatg aatggatccg
      541 gtttgccgac tgtcgccgtc gatattccct ccggcttgga cagcgacacc ggacagcggt
      601 tgggcgtggc gattcgagcc gatatgacgg tggctctggg ttatccaaaa ctcggcgaag
      661 tgattcatcc gggtctaact ttcgtcggcg agttggtcgt tgtcgacatc ggtatcgatc
      721 gccgcgcgct tgtcgaagtc gcgccgcaaa tcgagttgct cgatcgagaa accgttggct
      781 gggtggtgcc gcggcgccag gcggatacgc acaagggaac ctacggccac gtgctggtgg
      841 tcgccggcgc gcgcggaaaa accggcgcag cgattttgtc ttgccgcgcc gcgatgcgca
      901 ccggcgccgg cctggtgacc ctcgccgcgc cgcgggcgtt gaatgtaatt ttctccggct
      961 cgctggtaga agtgatgacc gagccattgg gcgaggatgc agccgagcag ctggagatgc
     1021 ttagcgacgc cgattggcgg cgcttgatcg aacgcaaaaa cgttttgctt tttggccccg
     1081 gcatcgggct cagcgacgcg acccaatcgg cgacgcgctg gctgttgcga aatttagcta
     1141 tgccgtgggt gatcgatgcc gacggtttga ataatctggc gcttgacttg gaccgcctgc
     1201 ggcaggccaa gacgccaccg atcctcacgc ctcatccggg cgagatggct cggctgatcg
     1261 gcaagagcac cgctgatgtc aacgccgacc gcatcgaggt cgcgcgcgga ttcgccacgg
     1321 cgcatcgctg tcatgtgatt ctcaagggcg cgcgcacggt gatcgccacg gcgggcggca
     1381 aggttttcct taatccaacc ggcaaccccg gcatggccag cggcggcatg ggcgatgtcc
     1441 tggcgggaat catcgccgca ctcttgggtc agggtttatc ggccgaggat gccatgaagc
     1501 tcggcgttta tttgcacggc tacgttggtg accgcgttgc cgaagccaag gggccgatcg
     1561 gaatgattgc gtcagatatc gtcgacggtc tgccacgagc gctggcggaa ctgggctcgt
     1621 cagcttccta acttgtcgtg ttgatctgcg agaccatagc tctggccgtc ggccggtctt
     1681 tttgaattcg aaataatcgc gcaagcaaga aggcgcaagc caaaaaggcc agccctgcgt
     1741 ctgtatctgt atcagaattc attgcgcgag attcgaggac tagccatacg ttaacttatt
     1801 ctttttccag ccatcaatat gcgtgcagga ttctttcagc taactctccc gcggcgagca
     1861 tggtcaggac aggttggtag ctgtgcgccg gctgtgaaat atcggcttac cgccaaacat
     1921 tttgggccgg acagcgatgc gattttgtcg gcttcgttcc taggtcgccc gccaagcgtg
     1981 aacgaatcat acgccagtaa gttagccttg aaatccgacc atgaacaaat attcgcaatc
     2041 ttgagcaaaa cgtggatcgt tatcggttcc gaatactcat tatccggaat ctcccggccg
     2101 gtctcgtcca caccgagtcg tcccttgaca ccttcgcgcg tcgcgcccta tgattcgccg
     2161 gtgttttcgg gctctagcaa atcggtgccg gcgcccgctt ccggtcagtt tggagggtgc
     2221 aatgaaacgc ttcgcatggg cgctcttggt tgtgctggcg tcggtcacct acgggcacgc
     2281 gcagacttcc ttctaccaag gcaagaccat taggatcatg gtcggcaacc ttgccggcga
     2341 cgcctacgat ctgtgggcaa gaatcttcgc ccagcacatg ggcaaataca ttgccgggca
     2401 tcccaacatc atcgtgcaaa acatgactgg agcgggcgga gtggtcgctg ccaactatgt
     2461 ttattcggtg gccaagccgg atggtctcac cctcggcaca ttcgggcctt cgatgtactt
     2521 cgatcaactg gccaggcgtc cgaaagtgcg atttgactgg gcgaaattca cttggctcgg
     2581 cacgccggaa caaaccgaat tcatcctggt catgaggacg gacactccgt accaatccat
     2641 cgaggatgtg cgcaaggcgg cacagccgcc caagtgcggc gccacaggca cgagcacttc
     2701 gggctattat atgccgaaat ttttggatga agggctgggc atcaagttca acatcgtcag
     2761 cggctatccc ggcggcggcg agattgatct cggcatcgag cgcggcgagc tgcactgccg
     2821 caatcttacg atcagcactt atttcgcccg cgagccgttc ctgagctggc acaagaaggg
     2881 atttgtccgg cccatcctgc aaaccggcaa gaagcgcgat ccgctcgtcg ccgatgtgcc
     2941 gtcgatctat gagttgatgg accaatacaa aaccccggag gcgacccggc gagtcgccaa
     3001 cgtcctgctt gcgcccgcgg tcttcggccg gccaatggtc gccaccccgg gtgtgccccc
     3061 cgatcgggtg aaaattttgc gcgatgccta ccaccaatcg ctgaaagatc cggcgctgct
     3121 cgaagagttg aagaagcgcc gttgggcggt ggatgcgatc agcggcgagg agttggcaaa
     3181 gctggcgaaa gaggtggtct cacagccgcc ggatgtggtc gaccgcatga tgaaattgtt
     3241 gggcaattga tttggcaccg ctgctggcgc gtttcgagac agtaacaaca aacgtatgga
     3301 cgagcctggc gtcggatcat ttgaaaattg ctctctgcgc acctcttggg tcggatcaat
     3361 caagccgccc gcggtgaaga ccgctagctc catcgtccgc ctagtagagt cacttactct
     3421 ttaaacgtcc aatctttggg aatgttctct taagctctcc agttaattta ccgtcaaccg
     3481 atctaaccac ggtggtcacc gcacaggcat gggacccgcc ccggaaagtt tcttgaccaa
     3541 ttcgttggtc tccgagtcgc gcgggatctc ccgaatcgcc tggtcgacct cttccggcat
     3601 caccggctcg aaatcctcct tgataatttt cttgtagtcg gcgagaaatt cgggatcttt
     3661 gaatgcccgc cgcatcgcct cccgcagtat ctctacccgg tctttgggcg tcccgggcgg
     3721 gacaacgaac ggcgccccgg cgtttctaaa agagcgaaat acggtaacga gccttcggtc
     3781 gctatctgat ttcgcgaaac tctcgagttc ggcaagtttg gcgaagcggg gatgcttcga
     3841 tcccttggga atctcgacga tggagtgaaa gttcatcgtc cccttgtcca gccagtccga
     3901 gttgcgcatc aaaatcgaag tcgcgagatt tgaccgggca tcgatctcgc cgcggagcag
     3961 ggcgacgtcg agttcgggac ccgtatagcc ggtggcaaat ttcggctctt ttaatcccat
     4021 ca
//
LOCUS       DASQKS010000663         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1238867, whole genome shotgun sequence.
ACCESSION   DASQKS010000663 DASQKS010000000
VERSION     DASQKS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238867"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VE170_17180"
     CDS             complement(<1..755)
                     /locus_tag="VE170_17180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYJ17229.1"
                     /translation="MAATEFDKLRNVGILGQGGSGKTSLGEAMLFAAGASQRLGKVLD
                     GTSVLDYEPEEIKHRVSISTALHSLAWKKHSITLIDTPGYAAFMADSVNSIRAMGAAV
                     FVLNPSVGVRVESERLWARANDEGVSRLLFVSKVDHEQANIRERIAPMLESLEAKGLY
                     LQMPIGTELGFKGVVDLLSMKAFIYEGDTGKYAEADIPAELKDEADEWREKLIEEISE
                     LDDALIEKYLDGKEFSIDEMKKAIREATRERRA"
     gene            complement(800..1576)
                     /locus_tag="VE170_17185"
     CDS             complement(800..1576)
                     /locus_tag="VE170_17185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HYJ17230.1"
                     /translation="MLLAIDIGNTNIVLGLYEGKQLITHWRLATQAERTADEYGVILT
                     QLVEHGGFRCAQISAIAVSCVVPPMLTTTQEFATRFFDREPLSVGPGIKTGMPILYEN
                     PKDVGADRIVNGIAAYEKYRDACIVVDFGTATTIDLISPKGEYIGGAIAPGLSISLEA
                     LVQRASKLPRIEIIKPKEVVGRNTVNSIQAGIFYGYVGLVDGLVKRIQKEQNVRVKVV
                     ATGGLAPLVASECATIDEVDEFLTLDGLRIIHERNAVTRQ"
     gene            complement(1587..2423)
                     /locus_tag="VE170_17190"
     CDS             complement(1587..2423)
                     /locus_tag="VE170_17190"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYJ17231.1"
                     /translation="MSEPKVVSLAQGTEPGPLDIAQITQGLGANRLATTIHYFPEIAS
                     TNSYAKERAESGAGEGELVVAESQSHGRGRLGRRWESPPFANLYVSIVLRPKLAPAHV
                     AQITLMAAVALAEVVEGFVPQKPAIKWPNDILIGGKKLAGILSEAACSAERVEYVILG
                     IGVNLNYACSAMPEELRRRATSVLEAAGRPVQRESFLQRLIQGIERCYGELEQAGFDA
                     MAARWESYFAWRGRRVRVELLDQVTTGTATGIDRDGALVIVDDRGAPQRILAGDVILA
                     EN"
     gene            complement(2416..3264)
                     /gene="nadC"
                     /locus_tag="VE170_17195"
     CDS             complement(2416..3264)
                     /gene="nadC"
                     /locus_tag="VE170_17195"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HYJ17232.1"
                     /translation="MDIHVSPQIERLIRDALDEDIGAGDLATMATISADARGKGLFRA
                     KKDGVAAGLVLLDRIFYFIDHRVEVSLLIKDGTAVKSGTVVAEVIGPVRALLLGERTA
                     LNFLQQLSGTATLTRKLVDAVKDFPCKVLDTRKTTPGLRTLQKYAVRMGGGTNHRIGL
                     YDAALVKDNHIEATGSIAEAVKAVRRNAPFMAKVEVEAGNMKQVQEAIDAGADVIMLD
                     NMNLAQMAEAVKLIDKRAGVEASGGITLESIRQVAETGVDFISSGALTHSAPVVDFNM
                     KITMNV"
     gene            complement(3269..>4183)
                     /locus_tag="VE170_17200"
     CDS             complement(3269..>4183)
                     /locus_tag="VE170_17200"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYJ17233.1"
                     /translation="QMHAPEADPLNREVAAAWERSDLALADRWIRSRLAATVSEARAA
                     IESYRFNDYANTLYQFIWHEFCDWYIEMSKLALSGADGDQIDGARRRLAGLLDQILLL
                     LHPVMPFVTEEIWQLMGNGRPSIMTQQFPQPQPQWVDDAAEKQMEFLMGAVRAIRNLR
                     TELNCPPGKEVKAIVCGQPADLDFLRVQAPFLRSLARVGSAEFRAAGDGPTGAATAVV
                     GTTEIYLPLHDLINLDEERSRLAKEVAKMEDEIARVQKKLDNSDFVAKAKEEVIQKER
                     DKAVQFDEKLRTLRSSLKKIEEFQAGRN"
BASE COUNT          846 a         1284 c         1150 g          903 t
ORIGIN      
        1 aacgcccgcc gctcccgggt ggcttcacga atggcctttt tcatttcatc gatggaaaac
       61 tcttttccgt cgagatactt ctcgatcaac gcgtcgtcga gctcggaaat ctcctcgatc
      121 aatttctcac gccactcgtc cgcctcgtct ttcagctcgg cggggatatc ggcttcggca
      181 tatttgcccg tgtcgccctc gtaaataaac gccttcatcg acaaaagatc gacgactccc
      241 ttgaagccga gctcggttcc gatcggcatc tgcaagtaaa gtcccttagc ttcgagagat
      301 tccagcatgg gggcgatgcg ttcgcgaata ttcgcttgct catgatccac cttactgaca
      361 aataacaacc ggctcacacc ctcgtcatta gcgcgcgccc ataaacgctc cgattcgaca
      421 cgcacgccga cgctgggatt gagcacgaac acagcagctc ccatggcgcg gatgctgttg
      481 accgagtcag ccataaacgc cgcgtaaccc ggagtgtcga tcaaggtgat gctatgtttc
      541 ttccatgcca aggagtgcaa cgccgtcgag atcgagaccc ggtgtttgat ctcttccggc
      601 tcataatcga gcaccgaagt gccgtctagg accttgccca gccgctgact cgcacccgcg
      661 gcaaacaaca tcgcctcgcc taaagaggtc ttccctgaac ctccctgacc caggatgccg
      721 acgttacgca gtttatcgaa ctcggttgca gccataaaaa ggattcctcc ttacaaattc
      781 tgccggttgc ctattttggt tattgcctcg ttactgcatt cctttcatga ataatgcgca
      841 ggccgtcaag agttaaaaat tcgtcgacct cgtcaatggt cgcgcactcc gacgccacca
      901 acggcgccag gccgccggtg gcgacgacct tgacccggac gttctgctcc ttttgaatgc
      961 gcttgaccag accgtcgacc agcccgacat aaccgtaaaa aatccccgcc tgaatcgaat
     1021 tgacggtatt gcgcccgacc acctctttgg gcttgatgat ttcgatgcgc ggcagcttcg
     1081 acgcccgctg caccagcgct tcgagggaaa tcgacaggcc gggcgcgatc gcgccgccga
     1141 tgtattcgcc tttcggggag atcagatcga tggtcgtagc cgtgccgaaa tcgacgacga
     1201 tgcaagcgtc acgatatttt tcgtaggcgg caattccgtt gacgatgcgg tcggcgccga
     1261 catccttggg gttctcgtaa agaatcggca tgccggtttt gatgccgggg ccgacggaga
     1321 gaggctcgcg gtcgaaaaac ctggtggcga attcttgcgt cgtggtgagc atgggcggca
     1381 ccacgcagga aaccgcgatg gccgagattt gcgcgcagcg aaagccgcca tgctcgacca
     1441 actgggttaa aattacgcca tactcatcgg cggtgcgttc cgcttgggtc gccaggcgcc
     1501 agtgggtaat gagttgcttg ccttcataga ggccaagaac gatgttggtg ttaccgatgt
     1561 caatggcaag cagcatgagg tgcgtgtcaa ttctctgcta atatcacatc gccggcgaga
     1621 atcctttgcg gggcgccgcg gtcatcaacg atcaccaggg cgccatcgcg atcgatgccg
     1681 gtggcagttc cggtggtcac ttgatcgagc aactcgaccc gtacgcgccg cccgcgccag
     1741 gcgaaataac tctcccaacg ggccgccatt gcatcgaagc cagcttgttc aagctctcca
     1801 tagcaccgct cgataccttg aatcaatcgt tgcagaaagc tttcccgctg caccggtcgg
     1861 ccggccgcct ccagcaccga agtggcgcgc cggcgaagct cttccggcat cgccgaacaa
     1921 gcgtagttga gattgacacc gatgcccaaa atcacgtatt ccacgcgctc ggccgagcac
     1981 gccgcttcgc tgagtatgcc ggcgagtttt ttgccgccga tgagaatatc gttgggccat
     2041 ttgatcgccg gcttctgcgg cacgaagccc tcgaccactt cagccagggc taccgccgcc
     2101 atcaatgtaa tctgcgccac atgggccggg gcaagtttcg gacgaagcac gatcgagaca
     2161 tagagattgg cgaagggggg cgattcccag cgccgtccca agcggccccg cccgtggctc
     2221 tgggactcgg cgaccaccag ctccccctct ccggcgccgg actcggcgcg ctccttggcg
     2281 tagctgttag tcgaagcgat ctcgggaaaa tagtgaatgg tggtagccag gcggttggcg
     2341 cccagcccct gggtgatctg ggcaatatcg agggggcctg gctcggtacc ctgcgccaat
     2401 gaaaccacct tgggctcaga cattcatggt gattttcatg ttgaagtcga ccaccggcgc
     2461 cgaatgggtg agcgcgcccg aggaaataaa atcgacaccg gtctcggcga cttggcgaat
     2521 cgattccaga gtgatgccgc cggaggcttc gacgccggct cgtttatcaa tcagcttcac
     2581 cgcttcagcc atctgcgcca ggttcatgtt gtcgagcatg atgacatcgg caccggcatc
     2641 gatggcctcc tgcacttgct tcatgttgcc cgcttcgact tcgactttgg ccatgaacgg
     2701 cgcgttgcgg cgcaccgctt tcaccgcttc ggcgatggag ccagtcgctt cgatatgatt
     2761 atccttgacc agcgcggcgt catagaggcc gatgcgatga ttggtgccgc cgcccatgcg
     2821 cacggcgtac ttctgtaggg ttcgcagtcc cggcgtggtc ttgcgcgtat cgagtacttt
     2881 gcagggaaaa tctttgacag catcgaccaa ctttctggtc aaagtcgccg tgccagacaa
     2941 ttgttgcaag aaattcagcg ccgtgcgctc gcccagaagc aacgcgcgca ccggtccaat
     3001 gacttcggcg accacagtgc cggacttcac tgcagtgccg tctttgatca gcaaactaac
     3061 ctcaactcga tgatcgatga aataaaaaat ccggtcgagc agcaccaaac cggcggccac
     3121 gccgtccttc ttggcgcgga acaacccctt accccgggca tccgccgaga tcgtcgccat
     3181 ggtcgccaga tcgccggcgc cgatatcttc gtccagtgcg tcgcggatca accgttcaat
     3241 ctgtgggcta acatggatat ccataaaatt aattccttcc cgcttggaat tcttcgattt
     3301 tcttcaaact tgaacgcagc gtgcgaagtt tttcgtcgaa ctggacggct ttgtcccgtt
     3361 ccttttgaat cacttcttcc tttgccttgg cgacgaaatc actattgtcg agtttctttt
     3421 ggacccgggc gatttcgtct tccatcttgg ccacttcctt ggccaagcga cttcgctctt
     3481 cgtcgagatt gattagatcg tggaggggca aatagatctc ggtggtgccg accaccgcgg
     3541 tagccgcccc cgtaggtccg tcgccggcgg cgcgaaactc ggcgctgcca acccgcgcca
     3601 gcgaacggag gaatggcgcc tggacgcgga gaaaatccag atcggctggc tggccgcaaa
     3661 ctatagcttt cacttccttg cctggtgggc aattgagttc ggtacgcaga ttacggatcg
     3721 cccgcaccgc gcccatgaga aattccatct gcttttccgc cgcgtcatcg acccattgcg
     3781 gctggggctg tggaaactgc tgcgtcataa tgctcgggcg gccattgccc atcagctgcc
     3841 agatctcttc ggtgacaaac ggcatgacgg gatggagcag tagcagaatt tgatccagca
     3901 ggccggcaag ccggcgacgc gccccatcga tctggtcgcc atcggcgcca ctgagcgcga
     3961 gcttgctcat ctcgatatac cagtcacaga actcatgcca aataaactgg tagagcgtgt
     4021 tggcatagtc gttgaatcga tagctctcga tcgcggcgcg cgcctcgcta acggtcgccg
     4081 ccaggcgtga tcgaatccaa cgatccgcta gagcaagatc gctgcgttcc catgccgccg
     4141 caacttcccg gttcaaagga tcggcctccg gcgcatgcat ctg
//
LOCUS       DASQKS010000664         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5832464, whole genome shotgun sequence.
ACCESSION   DASQKS010000664 DASQKS010000000
VERSION     DASQKS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5832464"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..935
                     /locus_tag="VE170_17205"
     CDS             192..935
                     /locus_tag="VE170_17205"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015165749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HYJ17234.1"
                     /translation="MTTTNTIELAATEITPDLEQAPAETVLRWALETFSPQIALACSF
                     QAEGSVLIDMIHRLRGSDFRIFTLDTGRLNQETYDCMDAIRARYGVGVEVYFPDALEV
                     QGMVRAKGLNLFYNSVEDRKLCCAVRKVAPLKRALKGLNAWMTGLRREQAVTRIAVPK
                     IEVDRGHDGIVKINPLADWSHDDVWSYIRAQNVPYNRLHTRGYPSIGCAPCTRAVQPG
                     EDFRAGRWWWENPDTKECGLHLNDEAISK"
     gene            1071..1529
                     /locus_tag="VE170_17210"
     CDS             1071..1529
                     /locus_tag="VE170_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYJ17235.1"
                     /translation="MISKKCKYALKALVRLGQEFGGELVLTDEIARSEHIPKKFLELI
                     LLDLKHAGYVRSKQGTKGGYRLVQPPSEISLAEVYRLFDGAIALLPCVSQKFYEECDD
                     CPDVKKCRLKEVFIDIREKTYELLAGVTIQSLLSGKSAAKSAIGQRKNHR"
     gene            1776..1994
                     /locus_tag="VE170_17215"
     CDS             1776..1994
                     /locus_tag="VE170_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17236.1"
                     /translation="MTVIELQIISMLKEAGSVSHEHLVQRVARDLYEGELRRGAAVVD
                     IGLFGSRLFEREVVETLKHGDGALWDIA"
     gene            2221..3243
                     /locus_tag="VE170_17220"
     CDS             2221..3243
                     /locus_tag="VE170_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ17237.1"
                     /translation="MFSRNISQQILLLATLLSALGAVSASNAHAQKTLLNVSYDPTRE
                     LYKKFNPAFGKYWQGKTGEAVSFKQAHGGSGAQARAVIDGLEADVVTLALAGDVDAIA
                     SKGLLATDWQKRLPQNSAPYTSTIVFLVRKGNPKGLRDWNDLAKPGISVITPNPKTSG
                     GAQWNYLAAWGYQLGKTGNNHAKAKELVTGIYKNLAVLDSGARGSTTTFVERGIGDVF
                     ISWENEAYLAVNELGKDKFEIVVPSVSILAEPTVAVVDKVVDKRGTRAIAQAYLEYLY
                     TPEGQEIAAKHYYRPRSVEIAKKYAKNFAQVKLFSIDDIFGGWKNAHKAHFADGANFD
                     QIYQQK"
     gene            3262..>3549
                     /locus_tag="VE170_17225"
     CDS             3262..>3549
                     /locus_tag="VE170_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HYJ17238.1"
                     /translation="MSRTFRQHSILPGFGLAMGFTVFYLSLIVLIPLGATFLKTFAGG
                     WNHFWEAVSGARAVASYRLTIGASLIAATVNAVLGLLTAWVLVRYNFPGKRI"
BASE COUNT          826 a          909 c         1028 g          786 t
ORIGIN      
        1 gccggcggca ttcgacgcgg tcaaaggcga cgtcgaggcg gtggcgaggg agttatacgg
       61 cggcgcagcg gtgagcgtgg agcgcgacgg cgattcgcac cgccaattga atctgcacgt
      121 aaaaagcccc gctgacattg atttgcaaaa ggtatgggag aatttgccgg caaactagat
      181 catggatgat tatgacaacg acgaacacca ttgagttagc cgccacagaa ataactccgg
      241 atctagagca ggccccggct gaaaccgtat tgcgctgggc actcgagacc ttttcgccgc
      301 aaattgccct ggcctgttca ttccaggccg agggatcggt gctgatcgac atgatacacc
      361 ggttgcgcgg cagcgatttc cgtattttca ctctggacac cgggcggctt aatcaggaga
      421 cctacgattg catggacgcg atccgagcgc gctacggtgt cggcgttgaa gtctactttc
      481 ctgacgccct tgaagtgcaa ggcatggtgc gggctaaagg gttgaacctg ttttacaact
      541 cggtggaaga ccgcaagctt tgctgtgcgg ttcgcaaagt cgcgccgctc aagcgcgcgc
      601 taaaaggtct aaatgcttgg atgaccggcc tgcgccggga acaggcggtg acccgcatcg
      661 ccgtgcctaa gattgaggtg gaccgggggc acgacggcat tgttaagatc aaccctctgg
      721 cggattggag tcacgacgac gtctggtctt acattcgcgc gcagaatgtt ccgtataatc
      781 gtttgcacac gcgtggctat ccgagcatcg gctgcgcgcc atgcaccaga gcggtgcagc
      841 cgggcgaaga tttccgcgcc gggcgctggt ggtgggaaaa tcccgatacg aaagaatgcg
      901 ggctgcacct aaacgatgag gcgatttcaa aatgagtttt cgccgccgag cggccgcggc
      961 ttttctacat ttggccgggc gttccttgtc cgggcaatgt gacaaagctt ggctttccca
     1021 gcctgcttat tatattgtcc ggatttgtag agcggacgga tccggactaa atgatatcga
     1081 agaaatgcaa atatgcgctg aaggcgctgg tgcggctggg tcaggagttt ggcggcgaac
     1141 tggtcttgac cgatgaaatc gctaggtcgg agcacatccc caagaaattt ctggagctaa
     1201 tcctcctcga tcttaaacac gctggatacg tacgcagcaa gcagggaacc aagggtggat
     1261 atcgtctcgt gcagcccccg agcgagatta gcttggcgga ggtttaccgc ttgttcgatg
     1321 gcgcgatcgc attgctgccg tgcgtgtcgc agaaattcta cgaagaatgc gacgactgtc
     1381 cggacgtgaa aaaatgccgt ctcaaggaag tgttcatcga cattcgtgaa aagacttatg
     1441 agcttcttgc cggcgtcacc atccaatctc ttctaagcgg taaatccgcc gctaaatccg
     1501 ctatcggaca aagaaaaaat catcggtaat attttctggg tttcacggtt gacaaaactg
     1561 gctgacccga atatctacta aatcgataga gttactggag atgaggcatt ttcgataatt
     1621 caggagatca aaagatgact gatttacaac gctcccgcgg gttcttgccc aagatctttt
     1681 cctttgcacg ggggaggact gattcgagcg atgccgcttc cgggatatcg actggaccca
     1741 ttgttcggtt ggtcaatacc agacgccgcg aggccgtgac ggtgatcgaa ctgcaaataa
     1801 tatcgatgct caaagaagcg ggatccgttt ctcatgaaca cctggtgcaa cgggtcgccc
     1861 gggatttgta cgagggcgag ctgcggcggg gagcggcagt cgttgatatc ggtttattcg
     1921 gcagccgctt gttcgagcgc gaagtggtcg aaacgctcaa gcacggcgat ggcgcactct
     1981 gggatattgc ttgagttcga gcgatcggtt tttgcccgaa tgctgcataa agccggatga
     2041 gcgaacaatt tcttaggaga cgacgagaat gaagcgaatt ttctctgcga tgcctaaaat
     2101 gcatgggcac attcacgcac tcatgcatcg gtgttgttga cgctcaatat tgcgtcacca
     2161 agtgccattg ccgatggcca aaccataacg ttcggagatc atcaattagg aacgataacc
     2221 atgttttcaa gaaatattag tcaacagatt ttattattag cgaccttact gagcgcgctg
     2281 ggtgccgtgt cggcgagtaa tgcgcacgcg caaaagacgc tgctcaatgt ttcctacgat
     2341 ccgacacgcg agctttataa aaaattcaat ccagccttcg gcaaatactg gcaaggcaag
     2401 accggcgagg cagtatcatt caaacaagcg cacggcggtt ccggcgcgca agccagggcg
     2461 gtgatcgatg gccttgaagc ggacgtggtg actctggctc ttgcgggcga tgtcgacgcg
     2521 atcgcgagca aagggctttt ggcaacggac tggcaaaagc ggctgccgca aaacagcgcg
     2581 ccctacacat cgaccatcgt cttcctggtg cggaaaggaa atcccaaagg tctgcgcgac
     2641 tggaacgatc tcgccaagcc tgggatatcc gtcataaccc caaatcccaa gacttccggc
     2701 ggcgcgcaat ggaactacct cgcggcttgg ggttatcaac tgggcaaaac cgggaataat
     2761 catgcaaagg ccaaagagct ggtgaccggc atatacaaaa atttagcggt gctggattcc
     2821 ggcgcgcgcg gctcgacgac caccttcgtt gaacgcggca tcggcgatgt ttttatctct
     2881 tgggaaaacg aagcttatct tgccgtcaac gaactgggca aagacaagtt cgagatcgtc
     2941 gtgccgtcgg tgagtattct cgccgagccc acggtcgcgg ttgtcgataa agtcgtcgac
     3001 aagcgcggca cccgcgccat cgcccaggcc tatttggaat acctctatac accggaaggg
     3061 caggagatcg cggctaaaca ttactaccgg ccgcgctcgg tggagattgc caaaaagtac
     3121 gccaagaact ttgcccaggt aaagcttttc agtatcgatg atattttcgg cggctggaag
     3181 aatgcgcaca aagcgcattt cgccgacggc gccaacttcg atcagatcta tcaacagaag
     3241 tagctggagg cgcaagcgac aatgagccgc acttttcgcc agcatagcat tcttccgggg
     3301 tttggcctgg ccatgggttt tacggtcttc tacctgagct taatcgtctt gattccgctc
     3361 ggagcgactt ttttgaagac ctttgccggg ggctggaatc atttttggga ggcggtgagc
     3421 ggtgcgcgtg ccgtggcatc ttaccggttg accatcggtg cttcgttgat tgcggcaacg
     3481 gtcaacgccg ttctcggact gctcactgcc tgggtgttgg ttcgctacaa tttccccggc
     3541 aaacggatc
//
LOCUS       DASQKS010000665         8523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4548271, whole genome shotgun sequence.
ACCESSION   DASQKS010000665 DASQKS010000000
VERSION     DASQKS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4548271"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VE170_17230"
     CDS             <1..253
                     /locus_tag="VE170_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17239.1"
                     /translation="DIAALLESEPVDMAKLEAKIREEEKLRTDLRIARIRAIEQSKAL
                     LNAEQKKKLSELQRQTLTLRFPPPSQNPTAPERGQPPRP"
     gene            377..1195
                     /locus_tag="VE170_17235"
     CDS             377..1195
                     /locus_tag="VE170_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ17240.1"
                     /translation="MKRVGQSKILRFILGSACAISLLLSPIPFAHSACRDPDVVRGFG
                     SLAYAANVDATIQYFGHNFFLITTSKGTRIVADPLGPGWYPNPNVAADVVTVGKEMFN
                     HNAVQIVLGNPLVLRGLKNYGADWNMINTSVKDTFIYSVPIHQNAESVQGIHGAAFVY
                     DLGFLCIAHLGDLSQKLNEQQIKAFGKVDIALTPIGGGRTMGPDLAKEVLAQLKPKIA
                     IPMHHRDNPYLIQQFTAGMKAQLLNTDTLAVSKDALPSPTEIRVLQPRGVMHYR"
     gene            complement(1352..2140)
                     /locus_tag="VE170_17240"
     CDS             complement(1352..2140)
                     /locus_tag="VE170_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ17241.1"
                     /translation="MRILRIIAIVIALLAAPVRVLPFCHELEVVQQEKVFRTALGSAA
                     QVTIEWLGHSTFQITSSKGTRVLTDPHGAFDLPRPTLPQHIVTSSHRHGPHSSVHMAP
                     GTPVIFHGLTPSGDDWQKISTTIRDVSVYVVPAYHDKSRGMQRGKNAIFVFKVDDICI
                     AHLGDLGHILTPDQLKMMGKIDILLVPIAGGYFTVTPSEARELTKLVNPKIAIPKHFW
                     WEQAVQEYTQGLTRVKMLSTPVLKISKPELPQPIETIVMPWGQR"
     gene            complement(2212..4473)
                     /locus_tag="VE170_17245"
     CDS             complement(2212..4473)
                     /locus_tag="VE170_17245"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HYJ17242.1"
                     /translation="MNNLYMIGSLPGWLVALIGVATAALLLQQYFGLKKRLPIGQSAI
                     LTALRALVYAVLIFFLFGPALIDRRVTKLRRPLTVLIDSSQSMGFPASAKSAPGDKNA
                     KSRLDLVRDKLDAGGNDSMLQKLSREYDLKLIRLGTSLEPISTGSLAGLQPQDPGTRL
                     LELLQTSGRDSAAAATGIIVFTDGITNGDKKSLDGGPALTVPVFAVGVGEAEGFTDVR
                     IAKLGAPEFAFRSREFKIDLTIQASGMKGKSVPLFFNRGKNIVTTRTVVVDADPFEQK
                     VTLAFTPKDLGTHGFSVSIPAQAGEQITQNNSKEFKVDVQRDKIRVLTLSGSPAWNYR
                     FLRMAMKQDPLIELVSFVFLRTPTDSVDVPESQLSLIPFPIDDIFLEELKNFDVIVFD
                     DFSHRAYFNPSYLERVRDFVRDGGGLAMFGGPRSFDAGGYGESALKEALPVELDGKGS
                     YQATSALSAALTPAGKAHPITRLLPDPKTNEEAWSKMPALTGMNQVRAGRGETLIVGS
                     AESGGAPLLTIGRFGKGRTLALMSDDAWRWNFIAVGNKETPQNHLKLVRQAVRWLAQE
                     PSFEQVQLRPIAAVQPGEKSQIKLKVLKDDFTPTRQASVQLRVFGPEGEPTAVAASAD
                     SEEGEYTGEFIPTKEGTHRVEAEASLGGKVLGKDRGSFTAAFAYGETDDGLPRLDLLK
                     QIAENSKGEYFSISDWNDKALEKIAAKLETIAPSQLVEQRQTQLWSTLWPFAIVLALL
                     SVEWWMRRKWGLI"
     gene            complement(4477..5247)
                     /locus_tag="VE170_17250"
     CDS             complement(4477..5247)
                     /locus_tag="VE170_17250"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HYJ17243.1"
                     /translation="MNRRNFLAVFIQTLLGATLVPPLARANHPIDKRRLQFVFAQLKY
                     RGGDWNPHPLSVTPLMEELMARTSVEAATVRHEVSLTDRDLFNYPFLYLTGKYEFDPF
                     TPEEIEVLRVFLTYGGFMLIDDALGQQGYGFDKAVRREMLRVFPGNEFKRMPAGHAAL
                     RSYYLLRRVGGVRLVSPALESITLGNATPVVYCQNGLCAAWERDQVGKWTNACTPGGE
                     PQRRDAFHLGINLILYAMTENYKDDLIHVPFIRKRLTR"
     gene            complement(5318..7405)
                     /locus_tag="VE170_17255"
     CDS             complement(5318..7405)
                     /locus_tag="VE170_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HYJ17244.1"
                     /translation="MSVFFLYPLFLFGLAAASLPVLIHLLNRRRLKRIRFPAVRFILL
                     SQKRISRSYRLRHWLLLALRTLAVICLALLLANPIFQTGAGLFAGGGPVSLVVILDNS
                     LSMTWSGDGTGFKRAQEAASLLINGLNDGDRAAVIPTNISGKQTFRLKDQKDVLLKEI
                     AGSEISDGSADVAAALSQAYELLNQPAGQKEIRIITDMGLTGWDQFSVAALKQADPSI
                     PLKVIRIGRKQQPVNGAVKEIRLGSAGVGVNLPLQIEATVINFGGSEIKELLVQLSID
                     GQNKEQKLATIPAKGETSVNFQTRIGQPGAHVGQITLKKDGFAGNAVAHFALDAQDKL
                     RILLVDGDPQTSLVQSETFFLTRALNPAGERDSSLFLPTVVLSDGLNAANLDNYQVVV
                     LANLATLPDPLVTKLQSYLRQGGGLLIFAGDKFSAESYNQKLAQALPAQLRDRKTGPE
                     AGGEKIDKIDLTHPALQSLADSILQESFKTVRVWGYQRAAGKALISLANGEPLLLEQK
                     VGAGKVMLMTTSADRDWSDLPVKTVFLPLIQALTQYLAGGKRGNLDAGIAVGAKKELS
                     FPANFVGRNLRVTKPDKQNTEVPIVGAKERATATVEENDKAGIYRLALAAGGDKDSGA
                     PQLYAVNAPFLESRLDEIGAAELAGKMQPIRTEVLDFEQLKDGGKRVDLALPLLAVLI
                     ATLLFEGWLGQRF"
     gene            complement(7413..8303)
                     /locus_tag="VE170_17260"
     CDS             complement(7413..8303)
                     /locus_tag="VE170_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYJ17245.1"
                     /translation="MADNNKNYFDPKVLAGIANLSLRARWVVEGLLSGVHKSRAKGFS
                     VEFEEHREYSPGDEIRRIDWKALGKFDRYMIKEYEDETNLRAYLMVDGSASMNYASDG
                     ISKFDYACTLAASLAYLVLKQQDAAGVITFSNKIESFIPPRSKRDYLLQIIHALENRG
                     PAGETNVAKILEELAGQIRRRGLIVLISDLLDEPEPILKGLRQFRFKGNDVIVFHLLD
                     QAELDLPFDGNILFEDLEAANLQVIADPRAIRATYKEVVQEFITDMRKQCHDSAIDYQ
                     LISTTTPLDQALASYLSWRG"
BASE COUNT         1720 a         2475 c         2500 g         1828 t
ORIGIN      
        1 cgatatcgcc gccctgctcg agagcgagcc ggtggatatg gccaagctcg aagcgaaaat
       61 tcgcgaggaa gaaaagctcc gcaccgactt gcgcatcgcg cgcatccgcg ccatcgagca
      121 aagcaaggcc ttgctcaacg cagagcaaaa gaaaaaacta agcgagctgc agcgccaaac
      181 gctgacgctg cggtttccgc cgccaagcca aaacccgacc gctccggaac gaggacagcc
      241 gccccgtcct taagccatct acagtttaaa atcgcagcac ctcaaaccaa ttgttttttc
      301 accgtcactg caatagcctg gctatgagga aatcccacgg cggtgacgcc ctgtttccgt
      361 cgtgatcgag agtgtcatga agcgcgttgg acaaagtaaa attctccggt tcatccttgg
      421 ctcagcctgc gctatcagct tactgctatc gccgatccca ttcgcgcatt ccgcctgccg
      481 tgaccccgat gtcgtgcgcg gcttcggatc cctcgcctac gcggccaatg tcgacgcgac
      541 gattcagtac ttcggccaca atttttttct gattacgacc agcaaaggaa cgcgcatcgt
      601 cgccgatccg ttgggacccg gatggtatcc caatcccaac gtcgccgccg atgtcgtcac
      661 ggtcggcaag gaaatgttca accacaatgc cgtgcagatc gtcttgggca atcccctggt
      721 cttgcgcggg ctgaaaaact acggcgccga ctggaacatg atcaacacga gcgtcaaaga
      781 taccttcatt tacagcgtgc cgattcatca gaacgccgag tctgtccagg ggattcacgg
      841 cgccgcattc gtctatgacc tcggattctt gtgcatcgcc cacttgggcg acttgagcca
      901 aaagttgaac gaacaacaga ttaaagcttt cggcaaagtc gatatcgcgc tcacgcccat
      961 cggcggcggc cgtaccatgg ggccggactt ggccaaggaa gtgctagccc agctcaagcc
     1021 taagatcgcc atcccgatgc accatcgcga caatccgtat ctgatccagc aattcaccgc
     1081 cggcatgaag gcccagttgc tcaacaccga taccctggcg gtgagcaagg atgcgctgcc
     1141 gtcgcccacc gagatccgcg ttctccagcc gcgcggcgtg atgcattacc gatgaacagg
     1201 atttatatct agctatttcg ttggcggaac ggcttggttt cgtctggcga cattcgtgcg
     1261 tgcgtagctt gaagtgacga gtttcgccat cgtggcccgg aggcgccggg cgcctctctt
     1321 tcctgaactc gttaaacttc ggccgatgtt tctaccgctg cccccaaggc ataacgatgg
     1381 tctcgatcgg ttgcggcagc tccggcttgg atattttcaa caccggcgtg ctgagcattt
     1441 ttactcgggt caggccctgg gtgtattcct gcacggcttg ttcccaccag aagtgtttgg
     1501 ggatggcgat tttgggattg actagtttcg tgagttcgcg cgcttcgctc ggtgtgacgg
     1561 tgaagtagcc gccggcgatg gggacgagca gaatgtcgat cttgcccatc atcttgagtt
     1621 gatcgggggt cagtatgtga cccaggtcgc ccaaatgggc gatgcaaata tcgtcaacct
     1681 taaaaacgaa gatggcattt ttgccgcgct gcatgccgcg gcttttatcg tgataggccg
     1741 ggacgacgta aaccgacacg tcgcggatgg ttgtcgagat cttttgccag tcatcgccgc
     1801 tcggcgttag cccgtgaaag attaccggcg tgccgggcgc catatggacg ctgctatgcg
     1861 ggccgtggcg gtggctgctg gtgacgatat gttgcggcag cgtcggccgc ggcaaatcga
     1921 aagctccatg cgggtcggtg agcacccgag tgcccttgga tgatgtaatt tgaaaggttg
     1981 aatggcccag ccattcgatg gtgacttgcg ccgcgctgcc gagggcggtg cgaaaaactt
     2041 tctcttgctg gacgacttcg agctcgtggc agaacggtag aacccgaact ggcgctgcca
     2101 gcagcgcgat aacaatagct atgatgcgga gaattcgcat cgtcgtttct ttgtaggggc
     2161 aaccccctgt ggttgcccga ccagggtagg cacgggggct cacccttacg atcaaatcaa
     2221 cccccacttc ctcctcatcc accactcgac gctgagcagc gccagcacga tggcgaaggg
     2281 ccaaagcgtg ctccacagct gggtttggcg ttgctcgacc agctgcgacg gcgcgatggt
     2341 ttcgagtttg gcggcgatct tctccagcgc tttgtcgttc cagtcgctga tcgaaaaata
     2401 ttcgcctttg ctgttctcgg caatttgttt caacaggtcg agccgcggca ggccatcgtc
     2461 ggtttcgccg taggcgaacg cggcggtgaa actgccgcga tctttgccca gcactttacc
     2521 gcccaagctt gcttccgctt cgacccgatg ggtgccctct ttggtcggaa tgaattcgcc
     2581 ggtgtactcg ccttcttcgc tgtcggcgct ggcggcgacc gccgtcggtt cgccttcggg
     2641 gccgaagacg cgcagttgca cggacgcctg gcgcgtcggt gtgaagtcat ctttcaggac
     2701 tttcaatttg atctggcttt tttcgcccgg ctgcaccgct gcgatgggac gcagctgcac
     2761 ttgttcgaag gacggctcct gggcgagcca gcgcaccgct tggcggacaa gttttaaatg
     2821 attttgcggc gtttctttat tgccgacggc gataaagttc cagcgccagg cgtcgtcgct
     2881 cataagcgcg agggtgcgtc ccttgccaaa gcggccgatg gtcaggagcg gcgcgccgcc
     2941 gctttcagcg ctgcctacga tcaaagtttc accacggccg gcgcgtacct ggttcatgcc
     3001 ggtgagcgcc ggcattttgc tccaggcttc ttcgttggtt tttggatccg gcagcaaccg
     3061 ggtgatcggg tgggctttgc ccgccggcgt gagcgccgcg ctaagcgctg acgtcgcttg
     3121 ataacttccc ttgccgtcca actccaccgg cagagcttct tttaaagcgc tctcgccgta
     3181 accgccggcg tcgaaggagc gcgggccgcc gaacatcgcc agcccgccgc cgtcgcgaac
     3241 gaaatcgcgc acccgttcca aataagacgg attgaaataa gcgcgatggg agaagtcgtc
     3301 gaacacaatc acatcgaagt tcttcaattc ttcgagaaaa atatcgtcga tcggaaacgg
     3361 aatgagactc agctggctct ccggtacatc gacgctgtcc gtcggcgtgc gcagaaacac
     3421 gaaggagacc agctcaatca acggatcctg cttcatcgcc atgcgcagaa aacgataatt
     3481 ccacgccggc gagccggaca acgtcaggac gcgaatcttg tcgcgctgga catcgacttt
     3541 gaattctttg ctattgttct gggtgatctg ttcgccggcc tgagccggta tgctgacgga
     3601 aaagccatgg gtgccgagat ccttgggcgt gaacgcgagg gtgacttttt gttcgaaggg
     3661 gtcggcgtcg acgaccacgg tgcgggtagt aacgatattt ttgccgcggt tgaagaacag
     3721 cggcaccgac ttgcctttca tccccgacgc ctggatcgtc agatcgatct tgaattcgcg
     3781 gctgcgaaag gcgaattccg gcgcgcccag cttggcaatg cgcacgtccg taaaaccttc
     3841 cgcctcgccg acgccgacgg caaataccgg caccgtcagc gccggtcccc catccaagct
     3901 cttcttgtcg ccattggtga tgccgtcggt gaagactatg ataccggttg ccgccgcggc
     3961 gctgtcgcgc ccgctggttt gcagcaattc caataatcgc gtgccgggat cctgcggctg
     4021 caaaccggcg agactaccgg tggagatcgg ctcgagactc gtgccgaggc gtatcagctt
     4081 gagatcgtat tcacgactga gtttctgcag catcgagtcg ttcccacccg catcaagttt
     4141 atcgcgcacg agatcgaggc ggctcttggc gtttttgtcc cccggcgcgc tcttggcgct
     4201 ggctggaaag cccatgctct gcgaactatc gatcagcacc gtcagcggcc gacgcaactt
     4261 ggtcacgcgc ctgtcgatca gcgccgggcc gaatagaaaa aaaatcagca cagcgtaaac
     4321 caaagcccgc agcgcggtaa gaatggcgct ttgaccgatc ggcagacgtt ttttcagacc
     4381 gaaatactgc tgcagcagca gcgccgcggt ggcgacgccg atgagcgcca ccaaccagcc
     4441 cggcagcgaa ccaatcatat aaaggttatt catggtttac cgggtgagcc gcttgcggat
     4501 gaatggcaca tggatgagat catctttgta gttctcggtc atggcataca aaatcagatt
     4561 aatgcccaag tgaaaggcgt cgcgccgctg cggctcgccg ccaggggtgc aagcattggt
     4621 ccatttgccc acttgatcgc gctcccaggc ggcgcacaga ccgttctggc agtagaccac
     4681 cggggttgcg ttgccaagcg tgatgctttc cagcgctggg ctgaccagtc gcacgccgcc
     4741 gacccggcgc aataaataat agctgcgcag ggcggcgtgg ccggcgggca tgcgtttgaa
     4801 ttcgttgccg ggaaataccc ggagcatctc acgccgcacg gctttgtcga aaccgtagcc
     4861 ctgttggcca agagcgtcgt cgatcagcat gaagccgccg taggtaagaa acacccgcaa
     4921 gacttcgatc tcctctgggg taaacggatc gaactcatat ttgccggtga gataaagaaa
     4981 cgggtaattg aacagatcgc ggtcggtcaa gctgacttca tggcgcaccg tcgcggcttc
     5041 gacgctggtg cgcgccatca gctcttccat cagcggcgtc accgacaacg gatgcggatt
     5101 ccaatccccg ccgcgatatt tgagctgggc gaaaacgaac tgcaaccggc gcttgtcgat
     5161 cggatgattg gcccgggcga gaggcggcac gagggtcgca cccagcagcg tctggataaa
     5221 cacagcgaga aagttgcggc gattcatacg ttaatagaaa aacggcttcg agccgagccc
     5281 atcgtagttt tgcttttact ttttccttct gcccttctta gaatctttgt cccagccagc
     5341 cctcgaatag caacgtggcg atcagcactg ccagtaacgg cagcgccagg tcgacgcgct
     5401 tgccgccatc tttgagttgc tcaaaatcga gcacttcggt gcggatcggt tgcattttac
     5461 cggcgagctc ggcggcgccg atctcgtcca ggcgcgattc caaaaacggc gcgttgacgg
     5521 cgtagagctg cggcgcgccg ctgtctttat cgccgccggc cgccagcgct aaccggtaga
     5581 tgcccgcctt gtcgttttct tcgactgtcg ccgtggcgcg ttccttggcg ccaacgatgg
     5641 ggacttcggt gttctgcttg tcgggtttgg tcacgcgcag attgcggccg acgaagttgg
     5701 ccgggaagga caattctttc ttcgctccga cggcgatgcc ggcgtccagg ttgccccgtt
     5761 tgccgccggc aagatattgc gtcagcgctt gaatcaacgg cagaaagacc gtcttgaccg
     5821 gcaggtcgct ccaatcgcgg tcggcgctgg tggtcattaa catcaccttg ccggcaccga
     5881 ccttctgttc aagcagcagc ggctcgccgt tggcaaggga gatgagcgcc ttgccggcgg
     5941 cgcgctggta accccacacg cgcacggtct tgaaggattc ctgtaaaata ctgtcggcga
     6001 gactttgcag cgccggatgc gtgagatcga tcttgtcgat cttctcgccg ccggcttccg
     6061 gtccggtttt cctgtcgcgc agctgcgccg gcaatgcttg ggcgagtttc tgattataac
     6121 tctcggcgga aaacttatcg ccggcgaaaa tcagcagacc gccgccctgg cgcagatagc
     6181 tctgcaactt ggttaccaac ggatcgggca gcgtcgcgag attggccaga acgaccacct
     6241 gatagttatc gaggttggcg gcgttgagcc cgtccgataa aaccactgtc ggtaggaaca
     6301 gcgatgagtc gcgctcaccc gccgggttga gcgcgcgagt gaggaaaaac gtttcgcttt
     6361 gcaccagcga ggtctgcgga tcgccgtcga ccaggagaat tctcaacttg tcctgggcgt
     6421 cgagagcaaa atgggccact gcattgccgg cgaagccgtc ctttttgagt gtgatttgac
     6481 cgacatgggc gccgggctgg ccgatacggg tttgaaagtt aaccgacgtc tcgcccttgg
     6541 cgggaatggt cgcgagcttc tgttctttgt tctgcccgtc gatgctgagc tgcaccaaca
     6601 gctccttgat ttccgagccg ccgaagttga tcaccgtcgc ttcgatctgc agcggcagat
     6661 tgacgccgac gccggcgctg cccaggcgaa tttctttgac tgcaccattg accggctgct
     6721 gctttctgcc gatgcggata actttcaacg ggatcgacgg gtcggcctgt ttcagcgcag
     6781 ccacggaaaa ttgatcccac ccggtgagcc ccatgtcggt gatgatgcgg atttcttttt
     6841 gccccgccgg ttgattgagc agctcatagg cctggctcaa agccgcagcg acatcggcgc
     6901 tgccatcgga gatttcactg ccggcaatct ctttcagcag cacatccttt tggtccttca
     6961 ggcggaaggt ttgcttgccg ctgatattgg tcggaatcac cgcggcacgg tcaccatcgt
     7021 tcaagccatt gatcaacagg ctcgccgcct cttgagctcg tttgaaaccg gtaccgtcgc
     7081 cggaccaggt catgctgagg gaattgtcca gtatcacgac cagcgacacc ggaccgccgc
     7141 cggcgaacaa accggcgccg gtttgaaaga tcggattggc gagtaggagc gcgagacaaa
     7201 tcaccgccag ggtgcgcagc gccaacaaca gccagtggcg caagcggtaa cttcgtgaaa
     7261 tgcgtttctg cgacagcagg ataaaacgga cggcgggaaa gcggatacgc ttcaagcggc
     7321 gccgattgag cagatgaatc aacaccggca acgacgccgc agccaggccg aaaagaaaca
     7381 gcgggtagag aaaaaaaacc gacatgcgag tcctaccctc tccagctcaa atagctcgcc
     7441 agcgcctggt ccagcggcgt cgtggtggaa atcaattggt agtcgatggc gctgtcgtgg
     7501 cactgtttgc gcatgtcggt gatgaactct tgaacgactt ctttataagt ggcgcggatc
     7561 gcgcgcgggt ctgcgatcac ttgaagattg gcggcttcca aatcttcgaa caggatattg
     7621 ccatcgaagg gcaagtcgag ctcggcctgg tcgagcaggt gaaagacgat tacatcgttg
     7681 cccttgaagc gaaactggcg cagccctttg agaatcggtt cgggctcgtc gagcaaatcg
     7741 gaaattaaca cgatcagacc gcgccggcgg atctgcccgg ccagctcttc gaggattttt
     7801 gcaacgttgg tttcccccgc cgggccgcga ttctccaaag catgaataat ttgtaataga
     7861 taatcgcgct tggagcgcgg cggaatgaag gattcgatct tgttcgaaaa agttattacg
     7921 cccgcggcgt cctgctgttt gagaaccaag tacgctaggg aggcggcgag ggtgcaggcg
     7981 taatcgaatt tgctgatgcc gtcggaggca taattcatcg atgcgctgcc gtcgaccatt
     8041 aggtaggcgc gtaggttggt ctcgtcctcg tactccttga tcatgtaacg gtcgaatttg
     8101 ccgagcgctt tccagtcgat gcggcggatc tcgtcgcccg gcgagtactc gcggtgctct
     8161 tcgaattcga cgctgaaacc cttggcgcgg cttttgtgta ccccggaaag caacccttcg
     8221 actacccaac gggcgcgcag cgagagattg gcgatgcccg ccagcacttt ggggtcgaag
     8281 taatttttat tgttgtcggc catcgcacgc tcgtcatgga caattacaac ctaccgattc
     8341 caaattgcag attcaaagga cctcggacaa ttggcaaccc ggaatggtga tctgtaatgc
     8401 cggcgctcta gcgcgccttg ccgtctgtcg aagcttttac atcggtcagc aggcggttga
     8461 cgatatcgag ggaagagagg ccttcacctt gggctttgaa gttcggcacg atgcggtggc
     8521 gca
//
LOCUS       DASQKS010000666         5484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2049598, whole genome shotgun sequence.
ACCESSION   DASQKS010000666 DASQKS010000000
VERSION     DASQKS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049598"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VE170_17265"
     CDS             complement(<1..760)
                     /locus_tag="VE170_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ17246.1"
                     /translation="MVKEIADNRRLIWLLIRRDISVRYRQSILGYVWAVGPPILTVGI
                     FAFLNASRVIPIGNTRIPYVAFALWGLSVWQLFAGCVSACTASLVSSGALVTKVNFPR
                     ETLVIAALGQPLGDFCVRLLPVTAVFVWYGVTPQWGLIFVPLLILPIMILALGFGFIL
                     SIANLVLRDTGNALGMALTVSMFLTPVLYPAPVRWPFFLVNVLNPLSPFVTATQDLIA
                     IGYINRPEMLTAACLFSLALAVGGWRVFHLTIPRV"
     gene            1047..2207
                     /locus_tag="VE170_17270"
     CDS             1047..2207
                     /locus_tag="VE170_17270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ17247.1"
                     /translation="MKILVLTKRRYMGKDLLDDRFGRFRELPLELARLGHQVRGLCLS
                     YRPRNEVRVIDATATRDGQVPWQSVNLTRGILPSLPAYFRQARRSIGEFQPDLIWACS
                     DAYHAIFGHRLAREFRCRLAIDLYDNFEAFSATKLPGVMFMFRRSVRKADGVTFFSQP
                     MAAHVIKTYPMTSPHTVILSGVRKDLFYPQDRNSCRKDLKLPAAAKIIGVAGALDSSR
                     DIDTLFSAFATLAEEMPNLHLALAGARSSKQKIPRGPKIHDFQSLPHERVPLFLNALD
                     LAVVSYRRSAQGKYSFPQKAYEIIACGVPLVAAAVGSMNELLRDYPNCLYEPEDAISL
                     ARAIRLQLEAKTAAQIIAPSWADSAAQLSRFFEKIAAPQLAKTERVNFSSAF"
     gene            complement(2204..3310)
                     /locus_tag="VE170_17275"
     CDS             complement(2204..3310)
                     /locus_tag="VE170_17275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ17248.1"
                     /translation="MEQLLASVCAGLSRNHRVSVITAYAEKHFAAGAETVFRPASPGL
                     IRYFLFALAKGAALLRGNRNITVVFGGSVLVTPIVLILARLFRRKAIVQAHGLDLIYV
                     GSIYQFFVVRGLRHVDHTIVNSRHTAGLARQKRVKARAIEVIPPGVDCQRFQVAEDPE
                     ALKQARGLANRKIILFVGRLVRRKGVKEFIEHSLADIVKQAPEVCFVIAGDNSTASLT
                     PHDDVAGDIRQAIEHHELSEQVCCLGAVSDVELVRIYALCDVLVLPVLDLDSDVEGFG
                     MVALEAAAAGKPVIATRCGGIPDAVNDGESGILVEPGNYSCLSEAIVSLLKNPLIAAS
                     MGQSGRQRAVKEFTWYSVVSRYEATFRRLSQLAN"
     gene            complement(3370..4095)
                     /locus_tag="VE170_17280"
     CDS             complement(3370..4095)
                     /locus_tag="VE170_17280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ17249.1"
                     /translation="MIPQPKTQDQPDVAALRDFYDRVYYAHASVTAQVPAHYRRLAKK
                     LQPWKAQRLLDVACGTGQWLGAATALGAIPAGIDISQRALDICRQAVPHAELHCGPAE
                     QLPFADRQFDVISCLGALEHFIDPRAALCEMVRVATPGAIVILLVPNAGFLPLRLGLY
                     SGTQQADVREEVRSLSAWSELFDSAGLRVRYRWRDLHVMSPSWIFRGPWHAWPVRAAQ
                     AFALPLWPLDWQYQVYHLCTIKR"
     gene            complement(4107..4994)
                     /locus_tag="VE170_17285"
     CDS             complement(4107..4994)
                     /locus_tag="VE170_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ17250.1"
                     /translation="MANAVETSIIVPVYNEAANLKKLLEQIHGLDLPQCEIIVVDDGS
                     SDGSAAAAMAAGANVVRHPYNIGNGAAVKSGMRAAKGKVIVLMDGDGQHKPEDIPRLL
                     AGVEIFHMVVGARAKGSKLRIHRNLANLLYNLLASYVTRFKVEDLTSGFRVMRRRDAL
                     RFIDLLPNTFSYPTTLTLAFLRSGLTVKYVPIQTLYRAGQSKISLVTDGILFLLIITK
                     IATLFAPFRVFLPVSVFFFLVGLGNYVYTYVTQNRFTNMSAFALSTAVVIFMLGLISE
                     QIALLRMERQESGRRQDDG"
     gene            complement(4987..5454)
                     /locus_tag="VE170_17290"
     CDS             complement(4987..5454)
                     /locus_tag="VE170_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17251.1"
                     /translation="MFALWHGAFLWKYVAAAAPWPYLTGAESRDGYLSRMLPEYPTFL
                     YINRETPLNAKIYLLFIGRRAYYCQRNYFHDGGELPGFLLGAIRNAKDSAQLEQSLRG
                     KQITHLMVRENLLTDFLNNNLSADQARLWNDFAASRLRLNFRERGHAVYQLNG"
BASE COUNT         1322 a         1562 c         1490 g         1110 t
ORIGIN      
        1 ccacacgtgg aatcgtgagg tgaaaaacgc gccagccgcc gacggctaac gctaacgaaa
       61 atagacacgc agccgtcaac atttcgggtc tgtttatgta tccgatagcg atcagatcct
      121 gggtcgcggt cacaaagggg ctgagcggat tcaggacatt aaccaagaaa aacggccagc
      181 gtaccggggc cgggtacagc acgggggtta gaaacatgct aaccgtgagc gccattccga
      241 gggcgtttcc agtatcccgc aggacaagat tggcaatcga taggataaag ccaaatccca
      301 atgccaagat catgattggc agaatcagca gtggtacaaa aattagcccc cattgaggcg
      361 ttacgccgta ccaaacaaaa accgcggtca ccggaagcaa gcgcacacag aaatcgccta
      421 gcggttgacc cagcgcggca atgacgaggg tctcccgtgg aaaattaacc ttggtcacca
      481 gcgccccgct cgaaaccaaa ctcgcagtgc aggcgctaac acaaccggca aaaagctgcc
      541 atacactcaa tccccaaagt gcaaaggcga cgtagggtat gcgggtgttg ccgatcggaa
      601 tgactctcga tgcgttcaga aaagcgaaaa tcccgacggt gaggataggc ggaccgacag
      661 cccaaacata accgagaatc gactggcgat agcgcaccga aatatcgcgc cggatcagca
      721 gccaaatcag ccgccgattg tcggcgattt ctttgaccat aagactccaa ccgttagcgc
      781 cattgcgctt gaccccagtc ggcgaatatt cgatctctgc gaggcttgac tcaaccggcc
      841 gtccgacggg atcggacgga ctagcacact gaatggtttg gtccattggt cgaaattcca
      901 ggtttattcc caatcgggaa tgtatcgccg agcgataacg gttattatag gtcctgttgc
      961 aggagattac tatttttttt tgaacatctg ggcacttcgg gtatattcag agtcgtcttc
     1021 cgaacgccat caaccatcca acgtcaatga aaatcctcgt ccttaccaaa cggcggtaca
     1081 tgggcaagga tctgctcgac gatcgcttcg gcagattccg cgagctgccc ttggaactgg
     1141 cgcgactcgg ccatcaggtt cgcggcctgt gtttaagcta ccgcccacgt aacgaagttc
     1201 gtgtcatcga cgccaccgcc acgcgggacg gtcaagttcc ctggcaatcc gtcaatttaa
     1261 cgcgcggtat cttgccgtca ctgcctgcat atttccgcca ggcgcgacgt tcgataggtg
     1321 aatttcagcc cgacctgata tgggcctgtt cagatgctta ccatgccatt tttgggcatc
     1381 ggctcgcgcg cgaatttcgc tgccggttgg caatcgatct atacgacaac ttcgaagctt
     1441 tttctgcgac caaactgcct ggcgttatgt tcatgtttcg ccgctcggtt agaaaagccg
     1501 acggagtcac tttcttcagt caacctatgg ccgcccatgt cattaagacc tatccgatga
     1561 cgagtccaca cactgtgatc ctgagcggtg tgcgcaagga tttattctat ccgcaggaca
     1621 gaaacagttg ccggaaagat ttgaaactgc ccgccgccgc caagatcatc ggcgtcgccg
     1681 gagcactcga ttcgagccgt gatatcgaca cgctattttc ggcatttgcg acattggcgg
     1741 aggaaatgcc aaatctccat ttggcgttgg ctggagcacg cagctcgaag caaaaaatac
     1801 cgagaggtcc gaagattcac gattttcaat ccttaccgca cgaacgagtt ccgctatttt
     1861 taaatgccct cgacctcgcc gtcgtaagtt atcgccgttc cgctcaaggc aaatatagct
     1921 ttccccaaaa ggcgtacgaa attatcgcct gcggtgtccc gttggtcgcc gctgcggtgg
     1981 ggtcgatgaa cgaattgctg cgggactatc cgaattgcct ttatgagccg gaagatgcga
     2041 taagcttggc acgcgccatc cggttacaat tagaagcaaa gaccgcggcg caaatcattg
     2101 caccctcgtg ggctgactcc gcggcacaac tgagcagatt tttcgaaaaa atcgccgcgc
     2161 cgcagcttgc caagactgaa agggtgaact ttagcagcgc attctaatta gctaattgac
     2221 tcaggcggcg gaacgtcgct tcgtagcgtg acaccaccga gtaccaggta aattctttca
     2281 cggcgcgttg gcggccggat tgtcccatcg atgcggcgat caggggattt ttcagcaaag
     2341 aaacgatcgc ctcgctcaag cacgaataat ttcccggctc gaccagaatg ccgctctccc
     2401 cgtcgttgac cgcgtcagga atcccgccgc aacgagtcgc gatcaccggc ttgccggcgg
     2461 cggccgcttc taacgcaacc atgccaaagc cctcgacatc cgaatccaga tccaagaccg
     2521 gcaacacgag cacatcgcat agcgcgtaaa tccgtactaa ctcgacgtcg ctgaccgcac
     2581 cgaggcaaca cacctgttcg gaaagttcat gatgctcgat cgcttggcga atatcgccgg
     2641 cgacatcgtc atggggcgtc aacgacgcag tagagttgtc gccggcgatg acaaaacaaa
     2701 cctcaggcgc ttgcttgacg atatcggcca gcgaatgctc gatgaattcc ttgacgccct
     2761 tacgccggac tagccggccg acgaaaagaa ttattttacg gttcgccaat ccccgcgcct
     2821 gtttgagtgc ttccgggtcc tctgcgactt gaaaacgctg acagtcgaca ccggggggaa
     2881 ttacctcaat cgctctcgcc ttaacacgtt tctggcgagc caatccagcg gtgtggcggc
     2941 tgttgacgat cgtgtgatcg acatggcgca gcccgcggac tacgaaaaat tgataaatcg
     3001 aaccgacata gatcaaatcc aagccatggg cttggacgat tgctttgcgg cgaaacaagc
     3061 gggccagtat caagacaatt ggcgtcacta acacgctgcc gccaaaaacc acggtgatgt
     3121 tgcggttgcc gcgcaaaagc gcggcccctt tggccaatgc gaagagaaag tagcgaatga
     3181 gtccgggcga cgcgggccga aacaccgtct ctgcccccgc tgcgaaatgc ttttcggcat
     3241 atgccgtaat cacggatacc cgatgatttc tgctcaagcc ggcgcacacg ctggccagca
     3301 gctgttccat ccctccctgc cgcggtggat aattccatgt gatgacgaga atctccatgc
     3361 tcgaagtggt caacgcttga ttgtgcacag atggtaaact tgatactgcc aatcgagcgg
     3421 ccaaagaggt agggcgaacg cttgagccgc ccgcaccggc cacgcgtgcc atggtccgcg
     3481 aaaaatccat gagggcgaca taacgtgcaa atcacgccag cgatagcgca ctctgagacc
     3541 ggcgctgtca aaaagttcgc tccacgcaga cagtgagcga acctcttcgc gaacgtcggc
     3601 ttgctgcgtt cctgaataga ggcccaggcg caacggcaaa aatcctgcgt tcggtacgag
     3661 gagaataacg attgcgcctg gcgttgccac ccgcaccatc tcgcacagcg ccgctcgcgg
     3721 gtctatgaaa tgttccaacg ctccgagaca agaaatcacg tcaaactgcc ggtcggcaaa
     3781 cggcaattgt tcggccggac cgcaatggag ctctgcgtga ggcactgctt gacggcatat
     3841 atcgagggct ctttgggaaa tatcgatgcc ggcgggtatc gctccaagcg cggttgccgc
     3901 gccaagccac tgacctgtgc cacaggcaac atcgagcaat cgctgggcct tccagggttg
     3961 aagcttcttc gccaggcggc ggtaatgcgc gggaacttga gccgtcacgc tggcgtgggc
     4021 gtaatagacc cgatcgtaga aatcgcgcag tgccgctacg tccggttgat cttgcgtttt
     4081 cggttgcgga atcacgctaa aggtaattaa ccatcgtcct ggcgccgccc gctctcctgg
     4141 cgctccatgc gcaggagggc aatctgttcc gagatcaaac ccaacatgaa aataaccacc
     4201 gccgtgctca aggcgaacgc cgacatgttg gtgaagcgat tctgagtgac gtaggtgtaa
     4261 acgtaattgc ccaggccgac gagaaagaaa aacacactca ccggcaagaa cactcgaaac
     4321 ggcgcgaaca acgtggcgat cttggtgatg atcagcaaaa agagaatgcc gtcggtgacc
     4381 aggctaattt tgctctggcc ggcgcgataa agggtctgga tcggcacata cttgacggtc
     4441 aacccggagc gcagaaatgc cagcgtcaga gtcgtcgggt aagaaaacgt attgggcagt
     4501 aagtcgatga aacgcagcgc atcgcgccgg cgcatgacgc gaaaaccgga ggtcaaatcc
     4561 tcgaccttga aacgggtcac ataagacgcc agcaaattgt atagcaggtt ggccagattg
     4621 cggtgtattc gcagcttcga gcccttggcg cgcgcgccga ccaccatgtg aaaaatctcc
     4681 actccggcca gcagtctggg aatatcctcg ggcttgtgct ggccatcgcc gtccatcagc
     4741 acgatgacct tgcccttggc cgcgcgcatg ccgcttttca ccgcggcgcc gttaccgata
     4801 ttgtagggat ggcgcacgac gttggcgccc gccgccatgg cggcagccgc gctgccgtcg
     4861 cttgagccat cgtcgacgac gatgatttcg cattgcggca aatcgagccc gtgaatctgc
     4921 tcaagaagtt ttttcagatt cgccgcttcg ttgtagacgg gaacgatgat cgaagtttcg
     4981 acagcgttag ccattcaatt gatagactgc gtggccgcgt tcgcggaagt tgagccgcaa
     5041 gcggctcgcg gcaaaatcat tccacaggcg ggcttgatcg gcgctcagat tgttattaag
     5101 aaaatctgtc agcagattct cgcgcaccat gagatgggtg atctgttttc ccctcaacga
     5161 ctgttccaac tgtgcgctgt ctttagcgtt acgaatcgcg cccagcagaa atccgggcag
     5221 ttcgccgcca tcgtggaaat agtttcgctg gcaataatac gctctccggc cgataaagag
     5281 aagataaatt ttcgcattca gcggcgtttc ccggttgata tagagaaacg tcggatactc
     5341 cggcagcatg cgcgaaagat agccatcacg gctctccgcg ccggttagat aaggccacgg
     5401 agcggctgcc gcgacatact tccagagaaa tgcgccatgc caaagcgcga acattcatag
     5461 gacggcgaat agaatcaccg gccg
//
LOCUS       DASQKS010000667         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2918776, whole genome shotgun sequence.
ACCESSION   DASQKS010000667 DASQKS010000000
VERSION     DASQKS010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918776"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VE170_17295"
     CDS             <1..635
                     /locus_tag="VE170_17295"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17252.1"
                     /translation="SIKRPEDLKGKRIGVSQLGSSTDFIARLAARRLGLTADKDVQIV
                     GVGGQGDRWTALTAGQIHATVLQSPFTLKARKANYPVFLDFSKEDFEYTIAGPLTTRT
                     FIRSDRETVMNFMRGLADGMDFYRDEKNKDRVHKYLGEYYRSNVIEELEETRRTYSQV
                     TPGLPLITAKAMENVIANDKNLAGTKLNVGELLDLSFLEQLAQERKAKGR"
     gene            804..1598
                     /locus_tag="VE170_17300"
     CDS             804..1598
                     /locus_tag="VE170_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYJ17253.1"
                     /translation="MAFETILFEVRNQIAFVTLNRPDSMNAVNRQMARELVEACKQVE
                     DDPGIRVVIFTGAGDKAFSAGMDLKERAETAFSPIERRNQKLSQTIHTQSRAFGAMTK
                     PTIAAIRGYCVGGGLEMALACDMRVAAEDAKLGLTEVRRGLIPGAGGTQRLPRAVGVS
                     KALEICLTGDTVSGSEGEKLGLVNKAVPAAEVMKTAEDLASRILKGAPMSVMFIKEAI
                     RKGVELSLEEGLRLEADLSAMVATTEDSKEGPKAFAEKRAPVWKGK"
     gene            1615..1752
                     /locus_tag="VE170_17305"
     CDS             1615..1752
                     /locus_tag="VE170_17305"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HYJ17254.1"
                     /translation="MGRSKADLPVEQPTTFQFIINLKTAKQIGVTIPQSVLFRADKVI
                     K"
     gene            1827..2645
                     /locus_tag="VE170_17310"
     CDS             1827..2645
                     /locus_tag="VE170_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYJ17255.1"
                     /translation="MADEKVLYKVENKIAWITLNRPESLNAVNRDMVKAIVGYCREAE
                     DDANVQIVIFKANGERAFSVGGDIKDRARQNAAEAAEPAPSPLVARQEKHNAIPGTPA
                     PAIAAMNKITIALLHGYTVGTGLLMSMACDIRIAAEGARLGVSEVRLGFMAGSGGTQR
                     LPRLVGLPKALEICLSGELYDAVECHRIGLVNQVVPYDQLIPAGEKMAQSFLKGAPLA
                     LRYIKEAIYKGLDMPFDQGLRYESDLQSMVMQTEDAKEGPKAFVEKRPANWQGR"
     gene            2775..4301
                     /locus_tag="VE170_17315"
     CDS             2775..4301
                     /locus_tag="VE170_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ17256.1"
                     /translation="MGTSIPMLMGDGLRRNGWKFPLKVAVRDKFRETTYGELNARVNR
                     LANGLLAIGVKRGDGVALSVGNRIEHLEIIFATAKIGALAIPLDVKWKALELASVVAA
                     LEPRFIILQEDCVGELAKAKELKDLSSIKAISLSADLSYSGLLDGQNADEPTLEVSED
                     DPFAVLLTSGTTGFPKGCLATQRTFVFHCINNAIEKGLGAHDKAILSSPIYFNAGRSF
                     TLGIIYYGGTMILHERFDAEEVLRTIERDKVTYVGAVPVMCERMLHVLESKKYDTSSL
                     RCLAITGGKVHPAVLESLQKNLTPNIYRTYASTDSGQMAISKPGDPPDKANSAGRPVW
                     CVDLRIVDDLGKPVQVGEVGEIICQSPLATHGYYKNPEATNASFRDGWFYTGDLGYFD
                     EEGFLFVSGRKKDMVKSGGISIYPLEIESVIYNHPDILEAAVIGVPDPQWGEALKAVI
                     VLKPGGKMDSAELLHFCRERLSAYKVPKSAEFVESLPHTEVGKVNKAQVREMILRRPT
                     "
     gene            4317..>5199
                     /locus_tag="VE170_17320"
     CDS             4317..>5199
                     /locus_tag="VE170_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17257.1"
                     /translation="MNPIGWLRIFGTRQLLWLGFGVLTLVMVAIGIIATLRVRSVTSS
                     FEKQTTVARPRSATARQLELNVLDYALGVHQYLKGHDLPRQRAITDATELARQIDAYT
                     ALAETARQRELAGRLRTAWQEVHALGESLMAAGAANREEVARFNETVVRLEQFFDSEL
                     QPDAVKAYEALNAKTLGSLQQTEIVTLSLLFFGLVIALVTGRAVGRAVLAGEAVLRDS
                     EERLTSLNATLEQKVDARTRELRELNADLDHRVVLRTRALGDSQKKLRALLEQLTRAE
                     EQERHRLATELHDYLGQL"
BASE COUNT         1183 a         1360 c         1603 g         1053 t
ORIGIN      
        1 cttcgatcaa acggccggag gatctcaagg ggaaaaggat cggcgtgagc caattagggt
       61 cgagtaccga tttcatcgcg cgcctggcgg cccgtcgttt agggctgact gccgacaaag
      121 atgtacaaat agtcggcgtg ggcggtcagg gagaccgctg gactgcgctc acggcgggtc
      181 aaattcacgc taccgtgctg caatcgccgt tcacgctaaa ggcgcgcaaa gcgaactatc
      241 cggtgtttct cgacttctcc aaagaagatt tcgagtacac catcgccggg ccgttgacga
      301 cgagaacttt tattcgttcc gatcgagaga cggtgatgaa cttcatgcgc gggttggcgg
      361 acggcatgga cttttaccgc gacgaaaaga ataaggatcg cgtgcataag tatctcggcg
      421 aatactatcg ctccaacgtg atcgaagagc tggaagagac gcgccggacc tatagtcagg
      481 tcaccccggg gctgccgctg atcaccgcca aggcaatgga gaacgtgatt gccaacgaca
      541 agaatctggc gggaacgaaa ttgaacgtcg gtgagctatt ggatttgtct tttctcgagc
      601 aactggcgca ggaacgaaag gcgaaggggc gttagcgcat cgtcgtaacg atgcgtcaga
      661 gatggtaacc gaaaaggatc caggttattt aagagatttc ttccgaatgg tcgaagtggc
      721 agcacctctc aataaccgaa tcggagaatg ttcattcgag acgctttgtc agttgaattt
      781 actattggaa aggcaggaag aaaatggcat ttgaaacaat tcttttcgaa gtgcgcaacc
      841 aaatcgcgtt tgtgaccctc aaccggccgg attcgatgaa cgccgtcaac cggcaaatgg
      901 cgcgcgaact tgtcgaggca tgcaaacaag ttgaagatga cccagggatt cgcgtcgtga
      961 tcttcaccgg cgccggcgac aaagcatttt ctgccggcat ggacttgaaa gaacgcgccg
     1021 agactgcgtt ctcgccgatc gagcggcgca accaaaagct ttcgcaaacg attcacaccc
     1081 agtcgcgcgc ctttggcgcc atgaccaagc ccacgatcgc cgcgatccgc ggttactgtg
     1141 tcggcggcgg ccttgaaatg gcgctcgcct gcgacatgcg cgtggcagcg gaagacgcca
     1201 agttggggct taccgaagtg cgccgtggct tgatccctgg cgccgggggt acgcaaaggt
     1261 tgccgcgcgc cgtcggcgtg tccaaagcgc tggaaatctg tcttaccggc gataccgttt
     1321 ccggcagtga aggtgaaaag ttaggcctcg tcaacaaagc cgttcccgcg gctgaagtga
     1381 tgaaaacagc cgaagatctc gccagtcgca ttctcaaagg agcgccgatg tcggtgatgt
     1441 ttatcaaaga agcgatcagg aagggcgtcg agttgtcact cgaagagggg ttgcgcctgg
     1501 aggccgacct ttcggcgatg gtcgcgacca cagaggatag caaagaagga ccgaaggcgt
     1561 tcgcggaaaa gcgcgcgccc gtatggaaag gtaaataggc aacggcatcc tacaatgggc
     1621 cgcagtaagg cagacctgcc ggtggagcaa ccgacgacat ttcagttcat cattaacttg
     1681 aagacggcca agcagatcgg cgtgacgatc ccccaatcag tgctgtttcg ggcggataag
     1741 gttatcaaat aaccaataga caacaggcaa aatgcaaccg gagggtgcgc ggaatactgc
     1801 gcccggaaaa tcgcaaggag atttatatgg cagacgaaaa agttctctac aaagtggaaa
     1861 acaagattgc ttggatcacg ctcaatcggc cggaatcgtt gaacgcggtg aaccgcgaca
     1921 tggtcaaggc gatagtcggc tattgccgcg aggccgagga cgatgccaat gttcagatcg
     1981 tgattttcaa ggccaacggc gagagggcct tctctgtcgg cggggatatc aaggaccggg
     2041 cgcggcaaaa tgccgccgag gcggctgagc cagcgccgtc gccgttggtg gcgcgccagg
     2101 agaagcacaa cgcgatcccc ggcacgccgg cgccggcgat cgcggcgatg aataagatca
     2161 ccatcgcatt gctgcatggc tacaccgtcg gcacgggatt gctgatgagc atggcttgcg
     2221 acattcgcat cgccgccgaa ggggcgcggc tcggtgtcag tgaagtgcgc ctcggtttca
     2281 tggccggttc cgggggcacg caacgtttgc cgcggctggt cgggctgccc aaagcgctgg
     2341 agatttgtct ttccggcgaa ttgtacgatg ccgttgagtg ccaccgcatc ggcctggtca
     2401 atcaagtggt gccctacgac cagttgattc cagccggcga aaagatggcc cagtcgtttc
     2461 tcaaaggcgc gccgctggcg ctgcgttata ttaaagaagc gatctacaag gggctcgaca
     2521 tgcctttcga tcaaggtttg cgctacgaat ccgacttgca gagcatggtg atgcagaccg
     2581 aagatgccaa ggaagggccc aaggcatttg tcgaaaagcg cccggcgaat tggcagggaa
     2641 gataactacc gttcaaggtt caagggttca aaagttcaag gtcagagtgg ctcagctaac
     2701 attgcggtga ttcgatcatt ccgtcgttga accttgaacc tttgaatttt tgaacggttt
     2761 gtagaaagcg tacgatgggc acaagtattc cgatgttaat gggtgacggc ttgcgccgca
     2821 acggctggaa gtttccgctc aaggtcgccg tgcgggacaa gtttcgcgaa acgacctacg
     2881 gcgagcttaa tgcccgggta aaccggcttg ccaatggctt gctcgccatc ggcgttaagc
     2941 gcggcgacgg ggtggcgttg tcggtgggca atcgcatcga gcatctggag attattttcg
     3001 ccaccgccaa gattggcgcc ctggcgatcc cgctcgatgt gaaatggaag gcgttggagt
     3061 tggcgtcggt agtcgccgct ctggaaccgc gtttcatcat tctccaagag gactgcgtcg
     3121 gtgagttggc caaggccaag gagttgaaag atctcagttc gatcaaagct atttcacttt
     3181 cggctgactt gagttacagc ggcttgctcg acggccagaa cgccgacgag ccgacgcttg
     3241 aggtcagcga agacgatccg tttgcagttt tgcttacttc gggtaccacc ggtttcccca
     3301 aaggctgcct ggcaacccag cggacctttg tctttcattg catcaataac gccatcgaaa
     3361 aggggcttgg tgcccacgat aaggcgattc tctcatcacc gatatatttc aacgccggcc
     3421 ggtcttttac cctgggaatt atttactacg gcgggaccat gatattgcac gagcgcttcg
     3481 atgccgagga agtgctcagg accatcgagc gtgacaaggt gacctacgtg ggcgctgtgc
     3541 cggtgatgtg cgagcgcatg ctgcatgtac tcgaatcgaa aaaatatgac accagctcgc
     3601 tgcgctgtct ggcgatcacc ggcggcaagg tgcatccagc ggtgctggag tcgttgcaga
     3661 aaaatcttac accgaacatt taccggactt acgcatcgac agattccggc cagatggcga
     3721 tctccaaacc cggcgaccca ccggacaagg cgaattctgc gggacggccg gtgtggtgcg
     3781 tcgatttacg tatcgtcgac gacctcggta agccggtgca agtgggggaa gtcggcgaaa
     3841 ttatttgtca aagcccgctg gcgacccatg gctattacaa gaatcccgag gcgaccaatg
     3901 cgtcatttcg cgacggttgg ttttacacag gcgatctcgg ctacttcgat gaggagggct
     3961 ttttatttgt ttccgggcgc aagaaagata tggtcaaaag cggcggcatc agcatctatc
     4021 cgctggagat cgagagcgtc atttacaacc accccgatat tctcgaggcc gccgtcatcg
     4081 gcgtgcccga tccgcagtgg ggcgaagcat tgaaagcggt gattgtgctc aagcccggcg
     4141 gcaaaatgga cagtgcagaa ctgctgcatt tttgtcgaga gcgtctgtcg gcgtacaaag
     4201 tgccgaagtc ggcggagttc gtcgagtcgc tgccacacac agaagtgggc aaggtgaaca
     4261 aagcccaggt gcgcgagatg atcttgcgtc ggccaaccta atcccggaga gtttgcttga
     4321 atccgatagg ttggttgcgt attttcggca cgagacaact gctctggctc ggctttggtg
     4381 ttctgacttt ggtcatggtg gcgatcggca tcatcgctac tcttcgggtt cgctccgtca
     4441 catcgagttt tgaaaaacaa accacggttg caagaccgcg cagcgcgacc gcgcggcaat
     4501 tggaacttaa cgtcctcgac tatgccctgg gcgtgcatca atacctcaag ggccacgatc
     4561 tgccgcgcca acgagcgatc acagacgcga ccgaattggc gcggcagatc gatgcttata
     4621 cggcgctggc tgaaactgcg cggcaacggg aactggcggg gcggctgcgc accgcttggc
     4681 aggaggttca cgctttgggc gagagcctga tggcggcggg tgcggcgaat cgcgaggagg
     4741 tagcccgctt caacgaaaca gtcgtgcggc tggagcagtt ttttgattcc gagctgcagc
     4801 cggatgcggt gaaagcctat gaagcgctca atgctaagac cctcggcagc ctccagcaaa
     4861 ccgagatcgt taccttgtct ttgctatttt ttggactggt gatcgcgttg gtgaccggcc
     4921 gggcggtggg acgggcggtg ctcgccggcg aagccgtgtt gcgcgatagc gaggagcggc
     4981 tgaccagcct caatgcgaca ttggagcaaa aggtcgacgc gcgcacgcgc gagttgcgcg
     5041 agctcaacgc cgatctcgat catcgggtcg tcctacggac ccgagcgctg ggcgattcac
     5101 aaaagaaact gcgggctttg ctggagcagt tgacgcgcgc cgaagagcag gagcgccatc
     5161 ggttggcgac cgagttgcac gactacctgg ggcaattgc
//
LOCUS       DASQKS010000668         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_806692, whole genome shotgun sequence.
ACCESSION   DASQKS010000668 DASQKS010000000
VERSION     DASQKS010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806692"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1084
                     /locus_tag="VE170_17325"
     CDS             116..1084
                     /locus_tag="VE170_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ17258.1"
                     /translation="MITAKDLKGALAIMPTPAKPGAERLDATDTVDLDETARLAEALV
                     RDGANGIMALGTMGECATTSQADYEAYVDCLLRTVRGRIPTFVGTTALGGHEIARRIK
                     FVKELGATGTLLGIPMWQPANVDMAVKFYADVAATFADFPIMVYANPRAFRFDFGVEF
                     WRQVIDKAPTVMSAKFSSKTILKQCVDVVKGRVNFVPPVGLAYEFAQISPASQTTCWI
                     PSVGPQVAIALMKALADGPAAQAKAVAADIAWANEPHHAITGSQEIFASYNIQLEKVL
                     MAASGYCKTGPIRPPYDYMPEDFRQAATEGGQRYAKLREKYSKVLG"
     gene            1242..1445
                     /locus_tag="VE170_17330"
     CDS             1242..1445
                     /locus_tag="VE170_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17259.1"
                     /translation="MTIDLTKAAAYSEKTPYDVWQDQDGIPICHGLGIDELKTLELVR
                     GSARPKPARCGETGQGQTLHARL"
     gene            1372..1743
                     /locus_tag="VE170_17335"
     CDS             1372..1743
                     /locus_tag="VE170_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17260.1"
                     /translation="MAAQGQSRRVAAKQDKVKRFMRAYSEGIFEFKTNRDKAIRIDAQ
                     WLKQKDLAVLDETHQFYGPNFSLPPRIDRNGIANTLDLVRQTSEVKGALNLGSFVDES
                     LIDELKRDGYYKKLTDPKAKK"
     gene            1762..2874
                     /locus_tag="VE170_17340"
     CDS             1762..2874
                     /locus_tag="VE170_17340"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17261.1"
                     /translation="MTEPKISPESITPKSSSYKKWVESEGIPLVESFFVDDIAQVPLA
                     PWKRTGGLGVRLCLAGTGETNDAYICEIPAGKSLAPEKHMYEELIYVIKGRGAATIWN
                     EGEPKRTFEWQEGSLFSPPLNAWHEIFNGNGSEPARFLAVTSAPCMINLIHNTDFIFD
                     CPYVFSDRYHSQENYFGNPGIWYSGRTWNTNFVADVKNLKLLEWKERGGGGSQVRLEM
                     SENTLCGHISQFAVGSYKKAHFHGPGAHVIILAGDGYSLLWPRGQEIKRFDWKPGSLV
                     VPPGGWFHQHFNAGSVPVRYLALRWGSQKYHEMWGEGRGKADVDVKLGGNQIEYEDED
                     PVVRTMFDQACAKAGVDNLMAKYYPNSRPNLPYESE"
     gene            complement(2899..3264)
                     /locus_tag="VE170_17345"
     CDS             complement(2899..3264)
                     /locus_tag="VE170_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17262.1"
                     /translation="MNAELAAQDRFAEFDVGPADFTAARGVAQAILLHLFRAGGVVHD
                     VDLKAPVIVASIGTRQVRCWSGDLKQVMFGQPRWLVCTQPLAKVERMSMPQSVSSAST
                     LIGISTGLLANSSDSRCAK"
BASE COUNT          816 a         1043 c          936 g          640 t
ORIGIN      
        1 cagttattca gcgttgccgc gatcgacccg acatgctaga gaggcgacta tgagaatccg
       61 ccagtgccct tggagcttaa cctagtcgtt actatttatt tataaggagg aaagtatgat
      121 cactgctaaa gatctgaaag gcgcgctcgc catcatgccg acgccggcaa aaccgggggc
      181 cgaacgtctg gatgcaaccg acactgtaga tctcgatgaa accgcccgcc tcgccgaagc
      241 attggtgcgc gacggcgcca acggcatcat ggcgctcggc accatgggcg agtgcgccac
      301 aacatcccag gccgattacg aagcctacgt cgactgcctg cttcgcacag tgcgcgggcg
      361 gataccgacc tttgtcggca ccacggcgct cggcggtcac gagatcgccc gccgcatcaa
      421 gttcgttaaa gaactcggcg ccaccggaac cctgttggga ataccgatgt ggcagccggc
      481 caacgtcgac atggcggtga aattctacgc cgatgtcgcc gccaccttcg ccgattttcc
      541 aatcatggta tacgccaatc cgcgcgcctt tcgtttcgac ttcggcgtcg aattctggcg
      601 acaagtgatc gacaaagcgc caaccgtgat gtcggcgaaa ttttcgagca agacgattct
      661 caaacaatgc gtggacgtcg tcaaaggccg ggtcaacttc gtgccgcccg tcggtcttgc
      721 ctacgagttc gcgcaaatat cgccggcgtc gcaaaccact tgctggattc cttccgtcgg
      781 accgcaggtc gccatcgcgc tgatgaaagc gctcgccgac gggcccgccg cgcaagccaa
      841 agccgtcgcc gccgacatcg cctgggccaa cgaaccgcac cacgccatca ccggctcgca
      901 agaaatattc gcctcctata acattcagct ggaaaaagtc ttgatggcgg catccggcta
      961 ctgcaagacc ggcccaatcc gcccgcccta tgattatatg ccggaggatt ttcgccaagc
     1021 cgccaccgaa ggcggccagc gttacgccaa gctgcgcgag aaatattcaa aagtcctcgg
     1081 ctagacgaac gcaccggcga atatcagtca gtcaccgcaa aggtcgcgaa acgggcgtaa
     1141 attaatgcgt ccctataaga aaccatcgtg cctttgtggt gactgactca ttcccgccga
     1201 tcttttgcgt cccttgcggc taattcatgg agggagctga agtgaccatc gacctaacca
     1261 aggcggccgc ctattcggaa aagacgccct acgacgtttg gcaggatcag gacggcattc
     1321 cgatctgcca cggactcggc atcgatgaac tcaaaacctt ggagctcgtc cgtggcagcg
     1381 caaggccaaa gccggcgcgt tgcggcgaaa caggacaagg tcaaacgctt catgcgcgcc
     1441 tatagcgaag gtatttttga gtttaagacc aaccgcgaca aagccatcag gatcgacgcc
     1501 cagtggctca agcaaaagga tctcgccgtt ctcgacgaga cgcaccagtt ttacggcccg
     1561 aatttttccc tgccgccgcg catcgatcgc aacggcatcg ccaacaccct cgatctcgtg
     1621 cgtcaaacca gcgaggttaa aggcgcattg aatctcgggt cgttcgtcga tgaatcgctc
     1681 attgacgagc tgaaacggga cggctattac aaaaaattaa ccgatccgaa agcgaagaaa
     1741 taagccagag agagagaaca catgacggaa ccgaaaattt ctcccgaatc gataacgcca
     1801 aaaagcagct cttacaaaaa gtgggtcgag agcgaaggca taccgctggt cgagagcttc
     1861 ttcgtcgacg atatcgccca ggtgccgttg gctccctgga aacgcaccgg cggcttgggc
     1921 gtgcgccttt gtttggccgg caccggcgag accaacgacg cctatatctg cgaaattcct
     1981 gccggtaaat ccctggcgcc ggaaaaacat atgtacgagg agttgatcta tgtcatcaaa
     2041 ggccggggcg cggcgacgat ttggaacgaa ggcgagccca agcgcacgtt cgaatggcag
     2101 gaaggctcgc tattctcgcc gccgctcaat gcttggcacg agatcttcaa tggcaacggc
     2161 agtgaacccg cgcgctttct cgccgtcacc agcgcgccct gcatgatcaa tctgattcac
     2221 aacaccgatt ttatcttcga ttgcccctac gtgtttagcg accgctacca ttctcaagaa
     2281 aactacttcg gcaatcccgg catctggtat tccggccgca catggaacac caatttcgtc
     2341 gccgacgtga agaatttgaa actgctcgaa tggaaagagc gcggcggcgg cggctcccag
     2401 gtgcgcttgg aaatgtccga gaacacactg tgcggccata tctcgcagtt cgccgtcggc
     2461 agctacaaga aagcccactt ccacggcccc ggcgcccatg tgatcatcct cgccggcgac
     2521 ggctattcgc tgctctggcc gcgcggccag gaaatcaagc gtttcgattg gaaacccggc
     2581 agcctggtcg ttccaccggg cgggtggttt caccagcatt ttaatgctgg ctcggtcccc
     2641 gtacgctatt tggcgctgcg ctggggcagc cagaagtatc acgagatgtg gggtgaaggc
     2701 cgcggcaaag ccgacgtcga cgtcaaactc ggcggcaacc aaatagagta tgaagacgag
     2761 gatccggtcg tacgaaccat gttcgaccaa gcctgcgcta aagccggcgt ggacaacctg
     2821 atggcaaaat actatccaaa tagccgaccg aatcttccat acgaatcgga gtaaaactcg
     2881 atggccgatc tcgctgacct atttcgcgca ccgcgaatcc gagctgttcg ccaacaaacc
     2941 cgtggaaatc ccgatcaaag tcgatgccga agaaaccgac tggggcatcg acatccgctc
     3001 gacctttgcc aatggctggg tgcacaccaa ccagcgcggc tggccgaaca tgacctgttt
     3061 caaatcgccg ctccagcatc tcacctggcg cgtgccgatc gacgcgacaa tcactggagc
     3121 tttcaggtcg acatcatgta cgacaccgcc ggctcggaaa agatgaagca atatcgcctg
     3181 cgccacgcct cgcgcggcgg taaagtcggc cggtcctaca tcgaactcgg cgaagcgatc
     3241 ctgcgcggcg agctccgcat tcaagacatc gacggcgacg acaaagcgaa catgatctgg
     3301 atccaggatt atgtcaccca gttgccaagg accgttgtcg gaacgcaaga gcgagcgctt
     3361 gattcgcgcc gacgtcgggc tcttgctcta tcgaaaactc tgggaacgag aagtcatggc
     3421 ccacgccgaa gggcg
//
LOCUS       DASQKS010000669         7167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4553618, whole genome shotgun sequence.
ACCESSION   DASQKS010000669 DASQKS010000000
VERSION     DASQKS010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4553618"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VE170_17350"
     CDS             <1..883
                     /locus_tag="VE170_17350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ17263.1"
                     /translation="RILQGAGAGCGVVLARAIARDVYGQDRVAQVIAYLTAAYVLGPM
                     LAPPIGGHLTVLFGWRALFVLASVVGLVVILAVAFAVPETRTRSGGAPRGVFAGYKSL
                     LRRPRFVGFMLQPGMMSAAFFTQATAASFLATEHLGADAAKIGLWFFAFPIGFMTGSF
                     ISGRVGARRSTEFMTILGGVIGVANGVLLIGWLYFGGISMAALYIPGMFVSLAQGLSM
                     PYAQAGAMAVDAELAGSASGAVVFSQLFWPAALQQLTGLLADGTWVPMATVHFAAVIV
                     ALLAGWLAARAEGESFN"
     gene            1282..2256
                     /locus_tag="VE170_17355"
     CDS             1282..2256
                     /locus_tag="VE170_17355"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17264.1"
                     /translation="MLIRNFVKWVVFLTVVVCLLPAQTGAQVDFPIGYVSRGGTYAFI
                     SLIEQHKLLEQEGIRPTFVYIGGPQVSQALISGDIKMAIVAAASPIRAAAHGADLRFV
                     GGITDTEVASFVSAANIKTPAELRGTRLAIDRIGDYSDFRARKVLEMFGLQAQKDVTL
                     LQIGAQTARFAALRNNQVQSTFVVPPLTLVARKAGLRELADLGELGFPSSSGAFVVMQ
                     STVERNPKEIYGVLRAVAKAIRIYKNDKEQALGAIARFMRLNDSEALEETRRMNVKVL
                     KDIPSPPVAGIRVVRDFLAQSDPEVKSMNLDAFITTQFSDRLQKEGLK"
     gene            2856..3482
                     /locus_tag="VE170_17360"
     CDS             2856..3482
                     /locus_tag="VE170_17360"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ17265.1"
                     /translation="MNLRHEYIAALTRELKDEIIVCNSGGVQDDLYSAADREANIYGP
                     GVGLVCQVALGLALAIPERTVIALDGDGSLLLNLGILPVLADQKPKNLFVIVFDNGVY
                     EAGGNVPTLASKGTKLEELARGAGVQGVTVQTSIEDFVTDLKAALSHDALRFFVVKVK
                     PGRVAGAPKTISGMENKFRFLRYVEQTLGKNIFQPPTQRFTGGKASDR"
     gene            3479..4435
                     /locus_tag="VE170_17365"
     CDS             3479..4435
                     /locus_tag="VE170_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17266.1"
                     /translation="MKHLCGILLSFLFYLDSVAAQELIKIGYPSTSYTTLPIVAAVRA
                     GFFAQEGLRVELIRMAPNISIIALVNNQVEFVTVQGSIIRGAARGLPIKSVAVIADRP
                     VYYLVARDAVTSINALKGKTIGLNSLGGSAHLMTKELLAHYRLDPDKDVTMIVSGDHK
                     SSIESVHVGQIDATVVPVPWQVVSKKAGLKVVAYYGDVLRMPLGGLGASDEFIQKRGD
                     TLKRVLRGTLKGIGFLRQKEKKSEVVKLIVDWFNVDRETAADSYDQIILAYPPDGRTS
                     TEVLEKDLEISRQLGAIKGTVPLSRVVNFKMLDEVARELGAK"
     gene            4522..5367
                     /locus_tag="VE170_17370"
     CDS             4522..5367
                     /locus_tag="VE170_17370"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HYJ17267.1"
                     /translation="MMAYPYDDSSLILALQIDHSRVAGYFAAHWGNKDFDRPTPYSSV
                     VLAAGEHDVGWWEWEMCPSTLNEKGYPLDYHDGSLKYLGQLRLDFYKNSVDSVMTRDP
                     YAAMLMAMHGVALMNAGYGKYAYPPDRTGDPRVKAYVDHQEALRLKLLGELRQSEKFK
                     QHCSDEKIWTNYEYMEVFDQLAQYVCNRYPLNSKARKLGPSDTLNDVDIPTKHAQPPV
                     KIKVDTVAANKAILRPYPFDIDPLPVSFTARLVPNRAYRDGEEFLGEFYRAARITVTH
                     TLASA"
     gene            5523..6407
                     /locus_tag="VE170_17375"
     CDS             5523..6407
                     /locus_tag="VE170_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYJ17268.1"
                     /translation="MKPANFEYHNPTTVAEALDLMARFGDQARPLAGGQSLVPLMNFR
                     LVRPAHVIDLNGVNGLNYLTVVDSALHIGATTRQRQLEKSVIVAQDWPLLRDAAAFIG
                     HIQIRNRGTVGGSLAHAFPSAELPIVMVTLGASMILRKQTGQRTIAAEEFFVSFMTTA
                     LEPKELLTEIQIPAPPVHCGWSYQEVSRRHGDFALAGAASVVAVDTNGRITHGRLTLT
                     GTTPIRARSVEESLLGEKPSEALFQTAAQRATQTLEQDSDIHASAAYRRSACTVLARR
                     ALAQAAQRSAISMKGTAR"
     gene            6404..6889
                     /locus_tag="VE170_17380"
     CDS             6404..6889
                     /locus_tag="VE170_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ17269.1"
                     /translation="MSSRQVRLTINGREYSGDVEPRKLLTDFLRDDLGLTGTHVGCEH
                     GVCGACTILLNGEPVRSCLLFAVQSNGATIKTIEGLAPNADELHPLQEKFWELHGLQC
                     GFCTPGILLTAEALLKEIPNPSEEQVREWISGNLCRCTGYQNIVTAILATAKAKNSPQ
                     S"
     gene            6986..>7167
                     /locus_tag="VE170_17385"
     CDS             6986..>7167
                     /locus_tag="VE170_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17270.1"
                     /translation="MAYIGSRIRRKEDLRLLRGIGKYVGDIHRPGMVHAAILRSTHAH
                     AHLLKIEAGAALKLPGV"
BASE COUNT         1608 a         1884 c         2062 g         1613 t
ORIGIN      
        1 gcgcatattg cagggagcgg gggcgggttg cggcgtcgtg ctggcgcgcg ccatcgctcg
       61 cgatgtctac ggccaggacc gcgttgccca ggtaatcgcc tatcttaccg ccgcctacgt
      121 gctcgggccg atgcttgcgc cgccgatcgg cggccacttg actgtgctgt tcggctggcg
      181 cgcgctgttc gtgttggcgt cggttgtcgg tctggtggta atcctcgcgg tggctttcgc
      241 ggtgccggaa acccgcacgc gcagcggcgg cgcgccgcgc ggcgtattcg ccggctataa
      301 atcgctgtta cgtcgcccgc gcttcgtcgg tttcatgcta cagccgggaa tgatgtcggc
      361 cgccttcttc acccaggcga ccgccgcatc atttctcgcc accgaacatc tcggcgccga
      421 tgccgccaag atcggcctgt ggttcttcgc cttcccgatc ggcttcatga ccgggagttt
      481 catctccggc cgcgtcggag caaggcgttc aaccgaattc atgaccatcc tgggcggcgt
      541 gatcggcgtc gccaacggcg tgctgctcat tggctggctt tatttcggcg gcatttccat
      601 ggccgcgctt tacatcccgg gaatgttcgt cagcctcgcc cagggcctgt ccatgcccta
      661 cgctcaagcc ggcgcaatgg cggtcgatgc ggagcttgcc ggttcggcgt cgggcgcggt
      721 ggtgttctcg cagctcttct ggccggccgc gctgcagcag cttaccgggt tgctcgcgga
      781 cgggacctgg gtgccgatgg cgacggtgca ctttgccgca gtgatcgtgg cattactcgc
      841 cggctggctg gcggcgcggg cggagggtga gtcgtttaat taattcaata tgcctatctc
      901 ttgttacgag ttatgccttc cgcccggctc tgtgggtaat cacgaatgat tcgtccgaac
      961 taaaatgatc ggaccgtgct ccgaggttgg ctcatccgaa atttcatcag ctttgatcaa
     1021 gtctatactt tgacattacg gatctgtcgg cctcatcgcg tgttcagcgc cgatgaggcg
     1081 ctggcggagc aagcaattag cgcgaacccg tacttggtag agacttactc aatagccggt
     1141 agactgcgtc aaggttttta gatagacatt tagccgacaa ttagttgtca ctgaatttta
     1201 aacatcacta ataggaagca aaaccgtggt tcggcgatat acggtattta gtatttccgc
     1261 ttgatgtcgg agtaactaag aatgcttatt cgaaattttg taaagtgggt cgtttttcta
     1321 accgtcgttg tctgtttgct gcctgctcag accggcgctc aggtcgactt tccaattggt
     1381 tacgtttcac gagggggcac ctatgcgttc attagcttaa tcgaacagca caaactgctc
     1441 gaacaggaag gcatacgccc gacgtttgtc tacataggag ggccgcaagt ctcgcaggcg
     1501 cttatctcgg gcgacatcaa gatggccatc gtcgccgcgg caagcccaat acgtgcggca
     1561 gcgcacggcg ccgatcttag gttcgttggc gggattaccg acacagaagt agcctcattt
     1621 gtctctgcag cgaatattaa aacccctgcg gagttaagag gaacgcgttt agctatcgat
     1681 cggataggtg attatagcga ctttcgcgct aggaaagtgc tcgagatgtt cgggttgcag
     1741 gcccaaaaag atgtcacgtt gctgcaaatc ggcgcacaga cggcacgctt cgctgccctg
     1801 aggaacaacc aggtgcaatc gacctttgtc gttcccccac tgacactggt tgcgaggaag
     1861 gcaggattgc gcgagcttgc cgacctcggc gaacttggct tcccgtcatc gtcaggagca
     1921 ttcgtcgtta tgcagtcgac tgtcgagaga aatcccaaag aaatctatgg agtgcttcgc
     1981 gcagttgcaa aggcgatacg catttacaag aacgataagg aacaagcctt aggcgctatc
     2041 gctcgtttca tgaggttaaa tgatagcgaa gccctggaag aaacgcggcg tatgaacgtg
     2101 aaagtactca aagacattcc ttcgccgcct gtagccggca taagagtcgt acgagacttt
     2161 cttgcgcagt ccgaccctga ggtaaaaagc atgaatctcg acgctttcat cacgacccag
     2221 ttctccgatc gcctgcaaaa agaggggctc aagtgatatt tctgtgtggc tgaggtttaa
     2281 ctatagggtt cgtcgcagct atcttcgtaa tggtcagggc gccggacctg tcgtcgctgt
     2341 gagagaggtt caatgaaatc agacgtcagt aatcagatcg tcgatgcact taaaggcatc
     2401 ggagtgcggc tagtggtatc gcttccggac agttgacttg gccggaccgg acagctcgct
     2461 gctcaggatc cggagctgat ccacgtctct gtcactaacg aaggcgatgg agtggccatc
     2521 tgtggcgggg cttggctggg agggaccaag gccgccatgc taatggagaa taccggcatg
     2581 ctcgtttcca cttatgcgct tacgagactc catctaacat tcggcatacc gacgcttatg
     2641 cttgtgtcgt accggggaga ttttggcgat ggcaattggt gggggtcttc tgtagggcgg
     2701 gttacagagc cggttttacg ggggctcggt atcagctacg aagtggtgcg ttcggcggat
     2761 gagattcccg gggctataag aaaggccgac cggagcagtg ctgcttcgct cctgccgacc
     2821 gcaatacttc tgggattcgg cagcgtgggg ggtgtatgaa tttacgtcac gaatatatcg
     2881 ccgccctcac ccgggaactt aaagatgaaa ttattgtctg caacagtggc ggcgtccagg
     2941 atgatttgta tagcgccgcc gatcgagagg caaatattta cgggcccgga gtcggattgg
     3001 tttgtcaggt ggcattaggg ctggcgttag cgattccaga gcgtacggtg atcgctttag
     3061 acggcgacgg cagtctgctt ctcaacctgg gaattctgcc ggtgctggcg gaccaaaaac
     3121 caaaaaatct ttttgtcatc gtttttgata acggcgtcta cgaggccgga ggaaatgtgc
     3181 ctaccttggc cagcaaagga accaagctcg aggaactcgc gcggggagcc ggagtccaag
     3241 gcgtaacggt gcagacgagc atcgaagatt ttgttacgga cctcaaggca gccctgagtc
     3301 atgacgcttt gcgcttcttc gtcgtaaagg taaaacccgg gcgagtcgcc ggcgccccaa
     3361 agacaatctc tggaatggaa aacaaatttc gctttttgcg ctacgttgag caaacgctgg
     3421 gtaaaaacat cttccagccg ccgacccaac gctttacggg tggaaaagcg agtgacaggt
     3481 gaaacatctg tgcggaatcc ttttatcctt cttgttctat ctcgattcgg ttgcggcgca
     3541 ggagctgata aagatcggct atccttccac tagctataca acactaccga ttgtcgccgc
     3601 ggttcgagct ggtttttttg cacaggaagg attgcgggtc gagctgatta ggatggcgcc
     3661 gaatattagc attattgcct tggtaaacaa tcaagttgag tttgtcaccg ttcaaggatc
     3721 gataatcagg ggcgcagcca gagggcttcc gatcaaaagc gtggccgtga ttgccgatcg
     3781 cccggtatat tacttggttg ccagagacgc cgttacgtcg atcaacgcgc tgaagggaaa
     3841 gaccatcggc ttgaattccc tcggcggctc ggcgcactta atgaccaagg aattactggc
     3901 tcattaccgg ctcgatccgg ataaagatgt tacgatgatc gtatccggtg atcataagag
     3961 cagtatcgaa tcggtgcatg tcgggcaaat cgatgcgaca gtagttccgg ttccgtggca
     4021 ggtcgtctca aaaaaagccg ggctgaaggt agtcgcctat tacggcgatg ttttgcgcat
     4081 gcctctcggt ggtctgggtg cttcggatga gtttattcag aagcgcggag acacccttaa
     4141 acgggtgcta agggggaccc tgaaaggcat cggctttctt cgccaaaaag agaaaaaaag
     4201 cgaggtggtc aaactcatcg tcgattggtt taacgtcgac agggagacgg cggcggactc
     4261 ctatgatcaa ataatccttg cctacccacc cgacggcagg acttcgactg aggttttgga
     4321 aaaagactta gaaatatcgc gacagctcgg cgctattaaa ggcacggtgc cgttatcgcg
     4381 cgtggttaat tttaaaatgc ttgacgaggt ggcccgcgaa ctcggtgcaa aatagccgcg
     4441 aacaatgaat ggttggtagc ggagtgttta acggaatacc gtcttaacct tgagatctca
     4501 atcttgtgag gagcaattct tatgatggcc tacccctatg atgactccag tttgatctta
     4561 gctctgcaaa tcgatcattc gcgcgtagca ggttactttg ccgcacactg ggggaataag
     4621 gactttgatc ggccaacgcc ttactcttcg gtggtcttgg ctgcgggtga acatgacgtc
     4681 ggctggtggg agtgggagat gtgcccgtcg accctaaacg agaagggata tccgttagat
     4741 tatcacgacg gcagtttaaa atatttgggc caattgcgcc tcgattttta caaaaactcg
     4801 gtggacagcg taatgacgcg cgatccgtat gcggccatgt tgatggcgat gcacggtgtg
     4861 gcgttgatga acgctggtta tggaaaatat gcctacccgc cggaccgcac cggtgatccg
     4921 cgcgtgaaag cctacgtcga tcatcaggaa gctctccgcc tcaagctgct cggggagttg
     4981 cgccaatccg agaagtttaa acagcattgt tccgacgaga agatctggac caattatgaa
     5041 tatatggagg tcttcgacca gctggctcag tatgtttgca accgctatcc attgaacagc
     5101 aaggcgcgca agctcgggcc gagcgatacc ttgaatgacg tcgatattcc gaccaagcat
     5161 gctcaaccac cggtaaaaat aaaagtcgac acggttgcag cgaacaaggc tatcctgcga
     5221 ccttatccgt ttgacattga cccgctacca gtttccttca ccgcgcggct ggtacctaac
     5281 cgagcctata gagacggcga agagttcctt ggcgagttct accgcgccgc gcggatcacg
     5341 gtaacgcata cgctcgcctc cgcttaggtc cgagaaacgg ccgccaccgc ctgctgatca
     5401 agcgaaaccg cagcgtcgta caatgaaatg ccgcgcgatc gctggacagt cgaacgcgtg
     5461 gattgatacg ggcaaataac ctagcgtttt taactaaccg aatagaaatc gaaaaattac
     5521 caatgaaacc ggcaaatttc gaatatcaca atccgacaac cgtcgccgaa gcactcgatc
     5581 tcatggcacg cttcggcgat caagcccggc cattggcggg cggccagagc ttggtgccgc
     5641 tgatgaattt tcgcctggtc cggccagcgc atgtgatcga tctcaatggg gttaacggtc
     5701 tcaattattt gacggtagtc gatagtgcgc tgcatattgg cgcgaccaca cggcaacgac
     5761 agcttgagaa atcggttata gtcgcccaag actggccgct tttgcgcgac gccgcggctt
     5821 tcatcggcca tattcagata cgtaaccgcg gcaccgtcgg cggcagttta gctcatgcct
     5881 tcccgtcggc ggaattgccg atcgtcatgg tcaccctggg tgcgtcaatg atcctgcgta
     5941 aacaaaccgg ccaacggacc atcgcggccg aagagttttt tgtttctttt atgactaccg
     6001 cgctggagcc aaaggaactg ctgaccgaga ttcaaattcc tgccccacca gttcattgcg
     6061 gttggtcata tcaggaggtg agccggcgtc atggggactt cgcccttgcg ggtgcggcat
     6121 cggtcgtcgc tgtggacacg aacgggagaa ttactcatgg caggctaaca ctcacgggaa
     6181 caacgccgat tcgagcccga agtgtcgaag aatcgctgct cggcgagaaa ccgtccgagg
     6241 cgctttttca gaccgccgct caacgcgcca cccaaacgct cgaacaggat tcggacattc
     6301 acgccagtgc ggcataccgc cgcagtgctt gcaccgtgtt ggcgcggcga gccctcgccc
     6361 aagcggccca acgatcagca atatctatga aaggaacagc ccgatgagca gccggcaagt
     6421 gcgcttgacg atcaatggcc gcgaatacag cggcgacgtg gagccgcgca aactcctgac
     6481 ggatttcttg cgggacgatc tcggtctcac cggcactcat gtcggctgtg agcatggcgt
     6541 gtgcggcgcg tgcacgatct tgttgaatgg cgaaccggtg cgctcttgtt tgctgttcgc
     6601 ggtgcagtcc aacggggcga caataaagac tattgaaggg ctggcaccga atgccgacga
     6661 actccacccc ctacaagaaa aattctggga actgcatggt ttgcaatgcg gcttctgcac
     6721 gccgggaatc ttgcttaccg ccgaagcact gttaaaagag ataccgaacc cgagcgaaga
     6781 gcaagtgcgc gaatggatca gcggaaatct ctgtcgttgc accggatatc aaaacatcgt
     6841 gacggcgata ttggcgacag ccaaggcaaa gaatagcccg caatcctgaa tagatagccg
     6901 agagtgacgg tagcaaaaac cgtggcgcag catgatttag caacagaaaa cccaaactcg
     6961 cgacgcgaac ccgaaactaa taattatggc gtacatcggt tcaagaatta gacgtaaaga
     7021 agatttgcgg cttttgcgcg gtatcggcaa atacgtgggc gacatccacc gtcccggcat
     7081 ggtgcacgcg gcgattcttc gcagcaccca cgcccacgcg cacctgctga agatcgaagc
     7141 tggcgcggcg ctcaaattac caggcgt
//
LOCUS       DASQKS010000670         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2052955, whole genome shotgun sequence.
ACCESSION   DASQKS010000670 DASQKS010000000
VERSION     DASQKS010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052955"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VE170_17390"
     CDS             <1..991
                     /locus_tag="VE170_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17271.1"
                     /translation="YGQAPFYQGKTITIVQGRDPGGTGDLRVRALVPFLQKYIPGSPT
                     IIMEFMPGGGSRKAANHVFRSSRPDGLTIGNLSSGMVSLAILGESGVLYDIDKFHYLG
                     SPYSSYHSVFVTRKDAGFDTIDKLRAATGVKVGGQSIGFSTYNEGRLFVYMLGLKEPL
                     FIAAFGGAELDPAMMRGEIDGRATGPDTILQRNRDWLDKGLANIHAIMETPKGDKHTH
                     PAFAKLPEIETFAKNDRERKMLALQRGFRVTGTPFVLPPGTPKDRVDTLKEAFRKTYL
                     DPEFAKYYLKLSGDEPSPLLPDNHEQAIREVPRDPEAIELFKIIAGPKKLPPR"
     gene            1194..2627
                     /locus_tag="VE170_17395"
     CDS             1194..2627
                     /locus_tag="VE170_17395"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYJ17272.1"
                     /translation="MADLRTWLDEVNKLGQLMTVNDAHWNLELSALTELINERSKTRP
                     AIVFDKIKEYPAGHRVAVNLLSSLQRLALTLGMDPNLAPFDFVQQWRKRVKSIQAVEP
                     RLVKSAPLFENVQKGADIDLFKFPVPKWHELDGGRYIGTDDLVITRDPEEGWVNVGTY
                     RIMIHGRDHMGLHMSPGKHGRVHRDKSHAEGKALPIAVSFGHHPINFLVASTDVPYRV
                     NEYAYAGGITGRPHDIVAGPLTGLPIPADSEIAIEGEVVPNDLMAEGPFGEWTGYYAS
                     NQPAVPVIRVKAIYHRNDPILCGFPLLKPSSGDNLHFSLMLSALIWNALDEAGVPDVQ
                     AVWSHPSGGRFMTVLAIKQRYPGHARQAAMIASQCRSGAYLGRYVIVVDEDIDITNTE
                     EVIWAISSRSDPVDSIEILRRCWSGPLDPRIPVGEKGFNSRAIIDATRPYEWRDKFPK
                     SSGHSRELKDKVAGDWQGFLNDKVGVR"
     gene            2686..3270
                     /locus_tag="VE170_17400"
     CDS             2686..3270
                     /locus_tag="VE170_17400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYJ17273.1"
                     /translation="MAKEAQKIYLIRHGETAWSRDGKHTGVTDVSLTDHGRQTARSLR
                     PVLAREQFALVLTSPLQRARETCELAGFAKDSRVEPDLMEWNYGDYEGLTTKQIQSAR
                     PGWSVFHHGCPGGESPEQVTVRADHMLAKVRAADGNVALFAHGHILRVIAARWVNQPP
                     SFGEHLLLDTATLSVLGYYYDTPAIKAWNAPIER"
     gene            complement(3395..>3617)
                     /locus_tag="VE170_17405"
     CDS             complement(3395..>3617)
                     /locus_tag="VE170_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17274.1"
                     /translation="FLFFNKGKKHTTIAFPNSNCGPSLYQSQPLCQNHFITGEEVFRL
                     LGLVSRLYFDDLPVLMVDRIVVAYDLLPD"
BASE COUNT          819 a         1047 c         1014 g          737 t
ORIGIN      
        1 ctatggtcaa gcgccgttct atcaaggcaa gacgatcacg atcgtgcaag gccgcgaccc
       61 cggcggcacc ggcgacctgc gggtgcgggc cttggttccg ttcctgcaaa aatatattcc
      121 cgggagtccg acgattatta tggaattcat gccgggcggc ggcagccgca aggccgccaa
      181 tcatgtgttc cgctcgtccc gtcctgacgg cctgaccatc ggcaacttga gctcaggcat
      241 ggtttccctc gccatactcg gggaatccgg agtgttatac gatatcgata agttccatta
      301 cttgggatcg ccctacagca gctatcactc ggtgtttgtc acccgcaaag acgccggctt
      361 cgataccata gacaaactgc gcgccgccac gggcgtcaaa gtcggcggcc agtccatcgg
      421 cttttccact tataatgaag gtcgtctctt cgtctacatg ctcggcctca aagaaccctt
      481 gttcatcgcc gccttcggcg gtgccgagct cgatccggca atgatgcgcg gcgagattga
      541 cggccgcgcc accggcccgg ataccattct gcaacgcaat cgcgattggc tcgacaaagg
      601 tttggccaac attcacgcga tcatggaaac gcccaaaggc gataagcata cccatccagc
      661 cttcgccaaa ctgcccgaga tcgagacctt cgccaagaat gatcgcgagc gtaagatgct
      721 cgccctgcag cgcggctttc gcgtcaccgg cacgcccttt gtgctgccgc cgggcacacc
      781 gaaagatcgc gtcgatactt tgaaagaggc tttccgcaag acctatctgg atcccgagtt
      841 tgctaaatat tatctcaaat tatccggcga cgaaccgtcc ccgttactgc cggacaacca
      901 cgagcaagcg atcagagaag tgccgcgcga cccagaggcg atcgagctgt tcaaaatcat
      961 cgccggtccg aagaagctgc cgccacggtg agctgaagaa agaagttcaa aacgttcaag
     1021 ccgttcaatc gctttacttc gttcaacccc actgttttct gctcaatggc agggaggaga
     1081 gtaatgtgag cgtaaacgct gcggtcaccc ttatcctcct tctcccaggg caggagtaaa
     1141 gtcgttgcag gttaagagtt gcagaatttt ttagcgagat tggaatattt attatggcag
     1201 acctacggac ttggctcgat gaggtaaaca aactcggcca gttgatgacc gtcaacgacg
     1261 cccattggaa tctggaactt tcggccctga cggaacttat caacgagcgg tcgaaaaccc
     1321 ggccggcgat tgtcttcgac aagatcaaag agtatccggc cggtcataga gtagcggtta
     1381 acttgctgtc gtcgttgcag cggctcgcgc tgaccctggg catggacccg aatttagcgc
     1441 catttgactt tgtgcagcaa tggcgcaagc gggtcaagtc gattcaagcc gtcgaaccgc
     1501 gcctggtcaa aagcgcgccg ctgtttgaaa acgttcagaa gggcgctgat atcgatttat
     1561 tcaagttccc tgtgccgaaa tggcatgagc tcgatggcgg ccgttatatc ggcaccgacg
     1621 atctggtaat cacccgcgat cccgaagaag gctgggtcaa cgtcggcacc tatcgcatca
     1681 tgattcatgg ccgcgatcac atgggactgc atatgtcgcc gggcaagcat ggccgcgtgc
     1741 atcgcgacaa gtctcacgcc gagggcaagg cgctgcccat cgcggtatcg ttcggccacc
     1801 atccgatcaa ttttctcgtt gcttccaccg acgtgcccta tcgggtgaac gaatacgcct
     1861 acgccggcgg catcaccggc cggccccacg acatcgtcgc aggaccgctc accggcctgc
     1921 cgatcccggc tgactcggag attgccatcg aaggcgaagt ggtgcccaac gatctgatgg
     1981 ccgaagggcc gttcggcgag tggaccggtt attacgccag caaccagccc gccgtgccgg
     2041 tgatccgtgt caaagcaatc tatcatcgca acgatccgat cctgtgcggc tttccattgc
     2101 tcaaaccgtc cagcggcgac aacctgcatt tttccttgat gctctcggcc ttgatatgga
     2161 acgccctgga tgaagcgggc gtgcccgacg ttcaggcggt ttggtcgcat ccatcgggtg
     2221 ggcgcttcat gacggtgctg gcgatcaagc agcgctatcc cggccatgcg cgtcaagcag
     2281 cgatgatcgc cagccagtgc cgctccggcg cctacctcgg ccgctacgtc atcgtcgtcg
     2341 acgaagacat cgacatcacc aataccgaag aagtcatctg ggcgatcagc tcgcgctccg
     2401 atcctgtaga ttccatcgaa atactccggc gttgctggag cggccccctc gatccacgca
     2461 ttcccgtcgg cgaaaaaggc ttcaattcgc gcgccatcat cgacgccacg cgcccctacg
     2521 agtggcgcga caagttcccc aagtcgagcg gccacagccg tgagctgaaa gacaaagtcg
     2581 ccggcgactg gcaggggttt ttgaatgaca aggtcggcgt cagataggtg ggcggaggga
     2641 atggccgcaa agaacgcaaa ggaacgcaaa aaatctgatt gaaggatggc taaggaagcg
     2701 caaaaaatct atttgatccg ccatggcgaa accgcatgga gccgcgacgg caaacatacc
     2761 ggcgtcaccg atgtgtcgct caccgatcat ggccggcaaa ccgcgcgatc gttgcggccg
     2821 gtgttggcgc gcgagcagtt cgctttggtg ctgaccagcc cgcttcagcg agcgcgcgaa
     2881 acctgcgagc tggcggggtt cgcgaaggac tcaagggttg aaccggactt gatggaatgg
     2941 aactacggcg actacgaagg actcacgacc aaacagattc agtcggcgcg gccgggttgg
     3001 tcggtgtttc atcatggctg tcccggcggt gaatcaccgg agcaagtcac cgtccgcgcc
     3061 gatcacatgc tcgccaaagt gcgcgccgcc gatggcaacg tcgcgctgtt cgcgcatggc
     3121 catatcctca gggtcatcgc ggcgcggtgg gtcaatcaac cgccgagttt cggcgagcat
     3181 ttattgctcg acaccgcgac gttaagtgtg ctcggttatt actacgacac gccggcgatt
     3241 aaggcttgga acgcaccaat cgaaaggtag tattcccggg gggggcgtga acgagatttc
     3301 gtgattgttt gcgcgcgggc actcgcttca gaacacttag tggtttgctt acgtgcaaaa
     3361 catcaagaca atcggactcg aacacgctaa attcttagtc gggtagcaag tcataggcaa
     3421 ctacgatccg atcaaccatc agaacgggaa gatcatcaaa gtaaaggcgc gagactaacc
     3481 ctaaaaggcg gaatacttct tcacccgtga taaagtgatt ctggcacaac ggttgtgatt
     3541 gatacaaact tgggccacaa tttgagttcg gaaaggcgat tgttgtatgt tttttaccct
     3601 tgttaaaaaa taagaac
//
LOCUS       DASQKS010000671         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5430624, whole genome shotgun sequence.
ACCESSION   DASQKS010000671 DASQKS010000000
VERSION     DASQKS010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5430624"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..808)
                     /locus_tag="VE170_17410"
     CDS             complement(215..808)
                     /locus_tag="VE170_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17275.1"
                     /translation="MLQQQVDASSSVTRNGEITEPTYAVALRVVGQDLAQLFPRVLEI
                     ETDGTVFEARGESHPNPFETIQEGFFARIWHKLRGIAPAQPAAAAASFSRRYELAEIE
                     RLDQLNRVHRLDNFRRADIYSLAERLRTMGAIVDGKNGRLKHLRKDADHLVVEYWDRQ
                     NLLQSAKLTTVILYRNQQQLDHQRRGGPSELWEGYDF"
     gene            complement(885..1877)
                     /locus_tag="VE170_17415"
     CDS             complement(885..1877)
                     /locus_tag="VE170_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HYJ17276.1"
                     /translation="MVATNFKIAILDDFEKVADTVPAYAKLKERADITLVRERLDSSE
                     KIVARLRDFDALLLMRERTFFSDKEYGQLPKLKFIAQTGRTSVHLDLANATKRGIAIS
                     GTPSDSGSPTKELTVGLILALARKIPQVNQRMREERWPAIAGLRLEGKTIGVLGLGRI
                     GNEVARIMKAFNTRVLGWSRSLTPERAAQVGAEAVPMQTLLKESDFVTVHVHFNAQTT
                     GLIGEKEIASMKRGAFLVNTGRGPIIDEKAMLAALESGHLGGVGLDVYDHEPLPMDHP
                     LRRCDNAILMSHRGYATVEMLSERYEQAITNLLAYLDGNPSSLINPDVQIRNAT"
     gene            complement(1882..2562)
                     /locus_tag="VE170_17420"
     CDS             complement(1882..2562)
                     /locus_tag="VE170_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17277.1"
                     /translation="MRKRIFNWLGHEFIELSGEATPGADASAEARELFQRFDTELHSH
                     GLSLDNTVRSRLWGRDRASRDAGSNERVKMLSGKARSASSSYIAPGHFDSAAKVAVDL
                     LAMRPPRAGLEKRIVEYDPPIVPIRFLVYDSVVVLSGVTTVLPTLAAQFDNILPRITG
                     SLGDAGSSWDKVAQVSFYFHRSQDLANLKTLFSHRVTATIPRLEYVIVDGYSSEGKFC
                     EVEVTATR"
     gene            complement(2700..2846)
                     /locus_tag="VE170_17425"
     CDS             complement(2700..2846)
                     /locus_tag="VE170_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17278.1"
                     /translation="MMKKLGAFFFFFVIIIAALGLANLLSITFYEKPLPDVIKDFLFI
                     LTRK"
     gene            complement(3212..>4432)
                     /locus_tag="VE170_17430"
     CDS             complement(3212..>4432)
                     /locus_tag="VE170_17430"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ17279.1"
                     /translation="LDDESFFDKRGTLPFAQELPKVKDNINVYGYPTGGTELSVTQGI
                     VSRIEYTDYYYQATGLRIQVDAALNFGNSGGPAVTDGKLVGLVFSLIQNAQNIGYLIP
                     VEEIQHFLTDIKDGVYDGKPQTHDLIQTVENDALRQRLGLPKGINGVMVAQPYRDDPS
                     YPLKEWDVITKIGDTPVDSDGKVEIRYDLRLSASYLVQKHAKNGTLPLTVFRKGKLLS
                     IDLPVASHRELVIPYLLDANPRYFIFGPFVFSQTTQDYLERLGNQRPTAFGRRPSPLV
                     IRRQDRPAFEDEELIVVASPLFPHRITKGYDDPNRSVVSEINGVPVRNMRHLVELLRD
                     NRDEQVSFKFASSGVLTHETMVFNRADLMNATGKILEENGIRYPYSADLRAVWEAAPA
                     GSKSAQATCCGLTP"
BASE COUNT          921 a         1170 c         1344 g          997 t
ORIGIN      
        1 gatgatcgcc tgcaacaacg ctgcgatatt ctggctgacg tcgtggtcgc gggtatcgcg
       61 ccaaaagatc tcggcgcgct gatcgtagtg gttgatcgtg gtcgtggaaa tgcggccgag
      121 ctctgcgcta ttgagtttta caccgctaac catgattcct gaacgaacca tgggcaaatt
      181 ttattttcaa gagaagcaga gccagcgggg gcgattagaa atcgtagcct tcccaaagct
      241 cggaggggcc gccgcggcgt tggtggtcga gctgttgttg gttgcgatac aagatcacgg
      301 tggtgagttt ggcggactgc aacagatttt gccggtccca atactcgacg accaggtggt
      361 cggcatcctt gcgcaggtgc ttgagtcggc cgtttttgcc gtcgacgatc gctcccatcg
      421 tacgcagccg ttcggctaaa ctgtaaatgt cggcacgtcg gaaattgtcc aagcgatgca
      481 cccggttgag ttggtcgaga cgctcgatct cggcgagttc atagcgccgg gagaaggaag
      541 cggccgccgc agccggctgg gccggcgcta tgccacgcag cttgtgccaa atcctggcaa
      601 agaaaccctc ttggatcgtc tcaaacggat tgggatggct ttcgccgcgc gcttcgaaca
      661 ctgtcccgtc ggtctcgatt tctaaaactc tgggaaataa ctgtgctaaa tcctggccca
      721 ccactcgcaa cgccacggca taggtgggct cggtaatctc gccgttcctg gtgacgctgc
      781 tagacgcgtc gacttgctgc tgtaacatga gcgaaatttt aatctgcttt gacggccaag
      841 gcaacggtta atagtgcgtg cagtaacgta ggcggctgaa ttggtcaggt agcgttgcga
      901 atttgcacat ccggattgat taagctggac ggattgccat ccaggtaggc caagagattg
      961 gtgatggctt gctcgtagcg ctcgctcagc atttcaactg tggcatagcc gcgatgggac
     1021 atcaggatgg cgttgtcaca gcggcgcagc gggtgatcca tcggcagcgg ttcgtggtca
     1081 tagacgtcga ggccgacgcc gcccaaatgg ccgctctcga gcgcggcgag catggccttt
     1141 tcgtcgatga tcgggccgcg tccggtgttt accaaaaacg cgccgcgttt catcgaagcg
     1201 atttcttttt cgccgattaa gccggtggtc tgggcattga aatggacatg gacagtgacg
     1261 aaatcggatt ccttaagcag ggtttgcatc ggcacagctt cggcgccgac ttgggcggcc
     1321 cgttccggcg tcaggctgcg cgaccagccg aggactctgg tgttgaatgc tttcatgatg
     1381 cgcgcgactt cgttgccgat gcggccgaga ccgaggacgc cgatggtttt accttccagc
     1441 ctcaggccgg caattgccgg ccagcgctcc tcgcgcatgc gttgattgac ttggggaatc
     1501 ttgcgcgcca gggccaggat caggccgacg gtgagttctt tggtcggcga gccgctgtcg
     1561 ctgggtgtac ccgagatggc gatacctcgc ttagtggcat tggccaaatc caaatgcaca
     1621 ctggtgcggc cggtctgcgc gatgaatttc aacttcggca gttggccgta ttccttgtcg
     1681 ctgaagaacg tccgctcgcg catcagcagc agggcatcga agtcgcgcag acgcgcgaca
     1741 atcttttcac tggagtcgag ccgttcgcgc acgagtgtga tgtcggcgcg ctctttgagt
     1801 ttggcgtagg ccggcacggt gtcggcgact ttttcgaagt cgtcgaggat ggcaattttg
     1861 aaatttgtcg cgaccatggt gctacctcgt tgcggtcact tcgacttcac agaactttcc
     1921 ttccgaggaa taaccgtcga cgatgacata ttccagtctg gggatggtcg ctgtcactcg
     1981 atgggaaaag agggttttca aattcgctaa atcctggctg cggtgaaagt agaatgaaac
     2041 ctgagcgacc ttgtcccagg agctgccggc gtcgccgaga gatccagtga tgcgcggcaa
     2101 aatattgtcg aactgcgcgg ccaaagtcgg cagtacggtg gtgacaccgg aaagcaccac
     2161 cacggaatcg taaactagga aacggatcgg cacgatcggc gggtcgtatt cgacgatccg
     2221 tttttctaag ccggctcggg gcgggcgcat ggcaagcaaa tcgacggcaa ccttggccgc
     2281 ggaatcgaaa tggcctggcg cgatgtagct tgagctggcc gaacgggcct tgcctgaaag
     2341 cattttcacc cgctcattgc tgccggcatc gcggctggcg cggtcccggc cccagagccg
     2401 cgagcgcacg gtattgtcga gggacagacc gtgggagtgc agctcagtgt cgaagcgttg
     2461 aaaaagttcg cgcgcctcgg cgctggcgtc ggcgccaggt gtagcttcgc ccgaaagttc
     2521 gataaactcg tggcctagcc agttaaatat tcgtttacgc atggcgggtt actccgatgt
     2581 gaacggcgcg atcctattac agggccgaat caggatcaac gccaatcgtg gatttctagc
     2641 tgcggtgaca gtgttgcggc agcagcctgg gcgcagatcg ctgccccggg acggtattgc
     2701 tactttctcg ttaggataaa caggaagtct ttgatgacgt cgggcagcgg cttttcgtaa
     2761 aacgttatcg acagaaggtt ggcgagtccg agcgcggcga tgatgatgac aaaaaagaaa
     2821 aaaaacgcgc ctaatttctt catcatattt gctccctggt tttgcagtcg atgcttttcg
     2881 gtcgtcgcgc tagcgggctg attaattgaa tttcacaggt agaaccagct cctcatgcgg
     2941 tcctggactg cccgccataa cgagctggcg agccaataaa gtgctttgtc cggtccctcg
     3001 ataaaatcgc gcagtcggcc gaatcggcgc ctgaaaggcc gcttcagttt ttccacttta
     3061 ctcgagctga cgctgcacaa gagttgcccg cctaatccgc tgaagagcat cgagcccgac
     3121 cgtaataatc aagccgacga gcaggaccct tttagggagt cggatcatgc gccgctttcc
     3181 cgccaactat cggatggtgt cggcgcaacg gttacggcgt taatccgcag caggtggctt
     3241 gggcggactt agacccggcg ggcgcagctt cccaaaccgc gcgcaggtcg gccgagtagg
     3301 gatagcggat accgttttct tcgaggattt tgccggtggc attcatcagg tcggcgcgat
     3361 tgaataccat ggtttcgtgg gtcaatactc ccgacgaagc aaacttgaaa ctgacctgct
     3421 cgtcacggtt atcacgcagc agttcgacca aatggcgcat gttgcgcacc ggcacaccgt
     3481 tgatctcgct gaccacagag cgattgggat cgtcgtagcc cttggtgatc cggtgcggga
     3541 acagcggcga tgcgacaacg ataagctcct catcttcgaa ggccggccgg tcttgacgcc
     3601 ggatcaccag aggactgggg cgccggccga acgcggtggg ccgttgattg cccagccgtt
     3661 ccaggtagtc ctgggtggtt tgagaaaaga cgaatggtcc aaatatgaaa tagcgcgggt
     3721 tggcatcgag cagataagga atcaccaact cccggtgtga ggcgaccggt agatcaatcg
     3781 ataagagctt gcctttgcga aatacggtga gcggcagcgt gccgttcttg gcatgctttt
     3841 gaaccaaata ggatgccgaa aggcggagat cgtagcggat ctctactttg ccgtcgctat
     3901 cgactggcgt gtcaccgatc ttggtaatga catcccattc cttcaacgga tagcttgggt
     3961 cgtcgcgata aggttgcgcg accatgacgc cgttgatgcc cttaggcagg ccgaggcgct
     4021 ggcgtagggc gtcgttctcg acggtttgaa tcaagtcgtg ggtttgcggt ttgccgtcgt
     4081 aaacgccgtc tttgatatcg gtgagaaaat gctgaatctc ctcgacggga atcagatagc
     4141 cgatgttctg agcgttttga atcagactaa agaccaaccc aactagtttg ccgtcggtta
     4201 ccgccgggcc gccgctgttg ccgaagttga gcgctgcatc gacttgaatg cgcaagccgg
     4261 tagcctggta gtaataatct gtatattcga tccgcgaaac aatcccttgt gtcacggaaa
     4321 gctcagtgcc acccgtagga tagccataaa cattaatgtt gtccttgacc ttgggcaact
     4381 cttgagcaaa gggcagtgtg ccgcgtttat cgaaaaaact ttcatcgtcc aa
//
LOCUS       DASQKS010000672         8212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2517985, whole genome shotgun sequence.
ACCESSION   DASQKS010000672 DASQKS010000000
VERSION     DASQKS010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517985"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VE170_17435"
     CDS             <1..1512
                     /locus_tag="VE170_17435"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYJ17280.1"
                     /translation="ATNMNKSMAKFFLIPYTVLAVALGVTLAPAMVGGQEGAAMPPAK
                     VERKNRAPVARDILAVKLPKPVEAKLKNGLTVLIVEDRRAPFVSVQLHVGGAGGLFEP
                     PAMTGLASVTAQMLREGTKTKNSIELAEYIDRLGASIGASTSFGSSDTVLSAAGLSDN
                     IDSWLGVAFEVLLQPSFAADELGKLKQRMRVQLREQRSNANFLLGERFNRAVYGEHPA
                     ANVSVTAESLDRITQDALRQWHRERYAPQNSILGIAGDIRARDVIVKLEKLLAGWKKS
                     ELRPIWPRDPQAASTRKVYLVDRPSSVQTTVSMGNIAIDRRSPDYLPMVVMNDIIGGG
                     ASARLFLNLREEKGYTYGVYSDFTAVHYPGPWRAGGNMRTEVTEGAMIEFFNEIRRIR
                     DEKVPPGELAESQRSIVASFALSLEQPSRALAFAITLKLYGLPADYWDTYAAKIAAVT
                     ATDVQRVARKYLDPDTLQIAAVGDAGKIKAVLEKYGAVEVYDSNGAPLPPGKS"
     gene            1652..4093
                     /locus_tag="VE170_17440"
     CDS             1652..4093
                     /locus_tag="VE170_17440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ17281.1"
                     /translation="MANSNPAAGALAGLRVLDLTGRMGGYCGLLLANLGAQVLLIETP
                     GGDSMRREGPFKNNTPHRERSLSFAAYHTNKHGAVLNLEDTRGSRKFRELAAHADVVI
                     EDKPFGYLDNLQLGYAELQKINPALVLTSISGFGRTGPYREFKAPSIVAFAMGGLMNL
                     CGHPGRAPLMGPCDVAYHLGSVHAAFGTMAALCNQSATGVGDHVDISLQDVLAADPFL
                     RIISRYSVTAEVPVRSGHSQSTTVAETYQCQDGYARIFVNQPEHWRRFVEWLGKPAEL
                     LDPKLENVQSRFALRTMIDRLVEARTLNYPVKKFFEEFQSNRLAAAPINPPSGYVYDE
                     QTKHRNYFVEVDHAEIGQHRFPGDPYKFSETPWRIVRGAPPLGASTDKIPDEFIGPSK
                     WREGAAGKERTQAEFAVAPTANAAMRKPLEGVRVISFPTGIVGPALAGLLAEQGAEVI
                     TIEAGRVIRSPQRGQRWQVAADFESNRERKRIAIDMKNPEGLGLAKQLIAKSDVVVEN
                     FSARVMASWGLDYPRMKEIRPDIIMASLQAFGQTGPRRDYVSFGPILMAYSGMTYLWR
                     DPQVERPGAASQTAFPDYVAPSYGAVAILAALIHRARTGAGQYIDISQAETAASMIGP
                     ALLELLVNGREPEPKGNFSATAAPHGCYRCKGDDRWCVIAIDNQDEWKRFCESIGHRE
                     WLADARFANPAARVVNRDALDAEIEAWTSTHTPHQLMMLLQRDGFAAGVVQTAEDLYR
                     DPHLRERGLARDVNHREIGWVTRAGPTARLSHNRVIADGPAHVAGEDNEAVLGELLGI
                     SAEDIRGLYERKVLR"
     gene            4149..5288
                     /locus_tag="VE170_17445"
     CDS             4149..5288
                     /locus_tag="VE170_17445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17282.1"
                     /translation="MLQGYRLFDADAHAMMSPRMWETLPQEFSARRPRPTRVHDAGDM
                     GRWTNGWLVEGQIIPHALGPGSQPGNEPSRVLEEFGAQTHSAEFPLGGFDLSDTRARL
                     RGLDYMGIDHQMLYPTTLYARMTNDPAFEAALMRSYNRYVGGQCAEAPQRLKWAGLLP
                     LRDAGQGCAALTEMQELGAAAAVVYGTAGDRLLCHPSFTPVWDALARSGLPLCVHMGM
                     SYPPFAEVCNGLLAAHGIGMSLPAQMAFVAIVGHGMLDRYPNLKVGFLEFGAEWMLYM
                     VPRLDHYLPIDRSQMPVKSEVPQRSIEEYAKSGRIFIAGEADDKLLPQEIELLGEDQI
                     LYSSDLPHGEGRHNAAKEIIARNDINERQKRKILYDNAVKFFGEP"
     gene            5360..5998
                     /locus_tag="VE170_17450"
     CDS             5360..5998
                     /locus_tag="VE170_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HYJ17283.1"
                     /translation="MNQAITPLDRAAIDLTIRQVERHLAKADPVMERLIAKHGHCPIA
                     EREFEPFHMLANSIISQQLSTRAAATIKQRVGVLVGVPFQIGKVLSIAPAELRGAGLS
                     GAKTRYISELAAHVSDGRLNFEEIVSLNDEAVIEKLIIVPGIGRWTAEMFLLFGLKRL
                     DVLAIGDAGLQRAARNLYGKKRSSATLLPRVAESWRPYRSIASWYLWRSLEI"
     gene            complement(6003..6632)
                     /locus_tag="VE170_17455"
     CDS             complement(6003..6632)
                     /locus_tag="VE170_17455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HYJ17284.1"
                     /translation="MAFSLEERVDPKHGMLIVVDMQNDFCHRDGAACKRGRDMAFVEK
                     MIPRLINLVNAARTKNFPICFVKTSGNQWTNSPVWTEFKNPELLACAEGTWGAEFHEG
                     LEPGPGETIITKHRYSAFIGTDLDMLLRARGVKSLLVTGVGTGMCVFHTLTVGFMLDY
                     YITLVEDCCATTYGPQAHSEAVALVKKHYGRVVSSQQIIETWIETKKPE"
     gene            complement(6641..7645)
                     /locus_tag="VE170_17460"
     CDS             complement(6641..7645)
                     /locus_tag="VE170_17460"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17285.1"
                     /translation="MKLTRHGLANLLAIFAFTLIGAQCVIAADRIRVGLSSFTPINAA
                     LWIAEEKGLFKKYAIESEVVLIGGASAGGVSSLIAGDVQFLGGGGGGVISAALSGADV
                     IMIGSIVNKGVQRVVARGDIRRPEDLRGKKIGITRLGAASHLVLLIMLRNWNMTANDV
                     QTLQIGSSPAMMAALEKGGIDAAVLTEPTFFFADDQGYRTLADLADMDIYYLHSMIDT
                     TRSYLRAHRDVAARFMKGYVEGIAYFKKNRKESIDVLARKLRTAPAQTKYLERSHTLY
                     TSGYFENAPYVSLKGVTTLLDFYGRDNPRAKTANPQSFFDNSLVKELDDSGFIKKLYE
                     "
     gene            complement(7668..>8212)
                     /locus_tag="VE170_17465"
     CDS             complement(7668..>8212)
                     /locus_tag="VE170_17465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17286.1"
                     /translation="NYPDLLDAKWKGKLLMDSTDYDWFGTLALAWGKTRAVNYMKQLA
                     RQDLTWRRGHGLVAQLIGAGELPLGWAYTFRVERMKKEGAPVDWSDTFDPIVTTVHGI
                     GVSSKAANPNSARLFVDFALSRRSQQMIRDLRLVPSRRDVEPLVAKMDQNRLKIKRIP
                     KEVSLNTDQHAKEFRELFGI"
BASE COUNT         1669 a         2381 c         2463 g         1699 t
ORIGIN      
        1 gcgaccaaca tgaataaatc gatggcgaaa tttttcttga ttccttatac cgtattggcc
       61 gtcgcgctcg gcgtgacgct ggcgccggca atggtgggcg gccaagaagg cgcagcgatg
      121 ccgccggcca aagttgagcg caaaaaccgc gcgccggtgg cgcgggatat tctcgccgtg
      181 aaacttccca agccggtgga agctaagctc aagaatggcc tgacggtttt gatcgttgaa
      241 gatcgccgcg cgccctttgt cagcgtgcag ctgcatgtcg gcggcgcggg cggtttgttc
      301 gaacctccgg cgatgaccgg cttggccagt gtcacggcgc aaatgctgcg tgaggggaca
      361 aaaaccaaaa acagcattga gctcgcggag tatattgacc ggttaggcgc gtcgattggc
      421 gccagcacat cctttggctc gtcggatacg gtgctgagcg ccgccgggct aagcgacaac
      481 attgacagct ggctcggcgt cgctttcgag gtcctgctgc agccaagttt tgccgccgat
      541 gaattgggaa agctcaagca acgtatgcgc gtgcagctgc gcgagcagcg ctccaatgcc
      601 aacttcctcc tgggcgagcg tttcaaccgc gccgtgtacg gcgagcaccc ggcggctaat
      661 gtgtcggtga cggccgagtc tctcgaccgc atcacccagg acgcgctgcg tcagtggcac
      721 cgcgagcgct atgcgccgca gaattcgatc ctcggcatcg ccggggatat tcgcgctaga
      781 gatgtaatcg tcaaattaga aaagctgctc gccggttgga agaagagcga actgcggccg
      841 atctggcccc gcgaccccca ggccgccagc acgcgcaaag tatacctcgt cgaccgtcct
      901 agctcggtgc agacaacggt ttccatgggt aacatcgcca tcgaccggcg cagtcccgac
      961 tacctgccga tggtcgtgat gaacgatatc atcggcggcg gcgcgtcggc gcgattattt
     1021 ctcaacctgc gcgaggaaaa aggttacacc tacggagtct acagcgactt caccgccgtg
     1081 cattaccccg gaccctggcg cgccggcggc aacatgcgca ccgaagtcac cgagggcgcg
     1141 atgatcgagt tcttcaacga gatcagacgc attcgcgatg aaaaagtccc gcccggtgaa
     1201 ttggcggaga gtcagcgctc gatcgtcgca agcttcgcac tgtcattgga gcaaccttcc
     1261 cgcgcattag cctttgccat tacgctgaag ctctacggtt tgccagccga ttactgggat
     1321 acctatgccg ccaagatcgc cgcggtgacg gcgaccgatg tgcagcgggt ggcgcgcaag
     1381 tatctcgatc ccgacacact gcagatcgcc gcggtcggcg atgccggcaa gatcaaagcg
     1441 gtgttggaaa aatacggcgc ggtggaggtt tacgatagca acggtgcgcc gttgccgcct
     1501 ggcaagtctt agcctgggcg gctaatttca gctttgccgg cggcgccaat gaatggtccg
     1561 tagcgcgtct gttggcggct tgtttcgcag gtaactttaa atatactccg agagcttgag
     1621 cgaaatcgcg ctctagatat ggagcttctc gatggctaat tccaacccgg cggccggcgc
     1681 cttggccggc ctgcgcgtcc tcgatctaac cggccgcatg ggcggctatt gcggcttact
     1741 gctagccaat cttggcgccc aagtattgct cattgaaacg ccgggcggcg attccatgcg
     1801 ccgcgaaggg ccgttcaaaa ataacacgcc ccatcgcgaa cgcagcttat ccttcgccgc
     1861 ttatcatacc aacaagcatg gcgcggtttt gaaccttgaa gatacccgtg gcagccgaaa
     1921 atttcgcgaa ctggcggccc atgccgatgt ggtgatcgaa gataaaccgt ttggctacct
     1981 cgataattta cagctcggct acgctgagct gcagaagatc aaccccgccc tggtgctgac
     2041 ttcgatcagc ggctttggcc gcaccggtcc ctatcgagaa ttcaaagcgc cgagcattgt
     2101 cgccttcgcc atgggcggac tgatgaatct gtgcggccat ccgggccggg cgccgctcat
     2161 gggaccctgc gatgtggcgt atcatttggg gtcggtgcat gcagccttcg gcaccatggc
     2221 cgcgctatgc aaccagagcg ccacgggtgt cggtgatcac gtcgatatat cgctgcaaga
     2281 tgttttggcc gcagatccgt ttttgcgcat catttctcga tacagcgtca ccgcggaagt
     2341 gccagtacgt agcggccata gccagtcgac cacggtggcg gaaacctacc agtgccagga
     2401 cggctacgcg cgcattttcg tcaaccagcc ggagcactgg cgtcgctttg tcgagtggtt
     2461 gggcaaaccg gcggaacttc tcgatccgaa actggagaat gttcagagcc gcttcgcgct
     2521 gcgcacgatg atcgatcgct tggtcgaagc gcgcacgctc aactatccgg tcaagaaatt
     2581 cttcgaggag tttcagtcca atcgtttagc cgcggcgccg atcaatccgc cgagcggtta
     2641 cgtgtacgat gagcagacca aacaccgtaa ctatttcgtc gaagtggatc atgccgagat
     2701 cggccagcat cgcttccccg gcgatcccta taagttttcc gaaaccccct ggcgtatcgt
     2761 gcgtggcgcg ccgccgctcg gcgccagcac cgacaagatc cccgacgaat ttatcggtcc
     2821 gtcaaaatgg cgcgaaggcg ccgccgggaa agagcgtact caagcagagt tcgccgtagc
     2881 gccgaccgcc aatgcggcaa tgcgaaaacc cctcgaaggc gttcgtgtta tcagttttcc
     2941 cacgggcatc gtcggtccgg cattggctgg actgttggct gaacaaggcg ctgaggtgat
     3001 taccatcgaa gccggccggg tcatccgcag cccgcaacgg ggccagcgtt ggcaggtcgc
     3061 ggcggatttc gaaagcaacc gcgaacgtaa acgcatcgcc atcgacatga aaaatcccga
     3121 agggctgggg ctggcaaaac aattgatcgc caaatcagat gtggtggtgg agaatttcag
     3181 cgcacgggtg atggcgagtt ggggcctgga ttatccgcgc atgaaggaaa ttcgcccgga
     3241 catcatcatg gcgagcttgc aggctttcgg tcaaaccggg ccgcggcgcg actatgtcag
     3301 cttcggtcca atcctcatgg cctattccgg catgacttac ctctggcgcg acccgcaggt
     3361 ggagcggccg ggtgcagcca gccagaccgc ctttcccgac tatgtcgcgc catcctacgg
     3421 cgccgtggcg atcctggccg cgctgattca ccgcgcccgc accggcgcag ggcaatacat
     3481 cgacatctcg caagccgaaa ccgccgcgtc gatgatcgga ccggcattat tggaattgct
     3541 ggtcaatggc cgagagcccg aacccaaggg caactttagc gcgacggcgg cgccccatgg
     3601 ctgttaccgc tgcaagggag acgatcgctg gtgcgtgatt gcgatcgaca accaggacga
     3661 atggaaacgt ttctgcgaaa gtatcggtca ccgcgaatgg ctcgccgacg cgcgctttgc
     3721 caaccctgcc gcgcgggtcg ttaaccgcga tgcgctcgat gccgagatcg aagcatggac
     3781 gagcacacac acgccgcacc agttgatgat gctgttgcag cgcgatggtt ttgccgccgg
     3841 cgttgtccag acggccgaag atttgtaccg ggatccgcat ctgcgtgagc gcggcctggc
     3901 aagggacgtg aaccatcgcg agatcggctg ggtcaccagg gccggcccga cggcacggct
     3961 atcgcacaac cgggttatcg cagatggccc ggcgcatgtc gccggagaag acaacgaagc
     4021 ggtattgggc gagctcctcg gaatatccgc cgaggatatt cgcggtttgt acgagcgcaa
     4081 agtgttgcgc tagccatgcc ctggtaagtt gccgctgccg tcgcagcaga ccgacgaagg
     4141 agagcaaaat gctgcaaggt tatcgcttat ttgacgcgga tgcgcatgcg atgatgagtc
     4201 cgcgcatgtg ggaaactctg ccgcaggagt tctcggcgcg ccggccgcgg ccgacgcggg
     4261 tccatgacgc cggcgacatg ggacggtgga ccaacgggtg gttggtcgag ggacaaatca
     4321 ttccacacgc attgggcccc ggcagccagc cgggcaatga accgtcgcgg gtgctcgaag
     4381 agttcggcgc ccagacgcac agcgcggaat ttcctctcgg cggcttcgat ctatccgaca
     4441 ccagggcacg actgcgcggc ctggattaca tgggcatcga tcaccagatg ctttatccga
     4501 ccacgctcta tgcgcgcatg accaacgatc cggctttcga agcggcgctg atgcgctcgt
     4561 acaatcgtta tgtcggcggc cagtgcgccg aggcaccgca acggctcaag tgggccggcc
     4621 tgttgccgct gcgcgacgcg ggtcaaggct gtgcagcgct gactgagatg caagagctcg
     4681 gagccgctgc cgcggtggtt tacggcaccg ccggcgaccg cttgctctgt catccgagtt
     4741 tcactccggt gtgggatgcg cttgctcgat cgggtctgcc gctttgcgtg catatgggca
     4801 tgagctatcc gccttttgcc gaggtctgca acggcctgct ggcggcccat ggcatcggca
     4861 tgagtctgcc ggcgcaaatg gcattcgtcg ccatcgtcgg ccatggcatg ctcgaccgct
     4921 atcccaattt aaaagtcgga tttctcgaat tcggcgccga gtggatgctc tacatggtgc
     4981 cgcgcctcga tcattatttg cccatcgacc gcagccagat gccggtcaaa agcgaagtgc
     5041 cgcagcgctc gattgaagag tacgccaagt cgggcaggat tttcatcgcc ggcgaagccg
     5101 atgataagtt actgccgcag gaaatcgagc tgctcggcga agatcaaatt ctctattcct
     5161 ccgacctgcc ccacggcgag ggccgccaca atgccgccaa ggaaatcatc gcgcgcaatg
     5221 acattaacga gaggcagaag cggaagattc tttacgacaa tgcggtgaag tttttcggcg
     5281 agccgtgatt agtaggtcta aagccgaaat tattcgctgg cgccacgtac agcagccgct
     5341 tcttgcagat ctcagaacga tgaatcaagc cattacacca ctcgacagag ccgcaatcga
     5401 cctgaccatt cgccaggtcg aacgccattt agccaaagcc gacccggtta tggaacggtt
     5461 gatcgccaag catggccact gccccatcgc cgagcgcgag ttcgagccgt ttcacatgct
     5521 cgccaactcg atcatcagcc aacagctttc gacacgagca gcggcgacga tcaagcagcg
     5581 cgtcggagtt ctagtcggcg tgccgtttca aatcggcaag gtgctttcca tcgcgccggc
     5641 ggaattgcgc ggtgccgggc tttcgggagc caagacccgt tacatcagcg agcttgcggc
     5701 ccatgtaagt gacggcaggt tgaatttcga agaaattgtt tccctcaatg acgaagcggt
     5761 gattgagaaa ctaattatag ttcccggcat cggccgctgg accgcggaga tgtttctgct
     5821 gtttggcttg aagcggttag acgtgctcgc tatcggtgac gccgggttac aacgtgccgc
     5881 gcgaaatctt tacggcaaaa agcgcagttc cgcgacattg ttgccgcggg ttgcggaaag
     5941 ctggcggcct tatcggtcga ttgcgtcatg gtatctgtgg cgatctttag agatataagg
     6001 cttcactcgg gtttctttgt ttctatccaa gtttcgataa tctgctgtga cgacacgact
     6061 cgcccgtagt gttttttcac caaagccact gcttcactgt gcgcttgcgg cccgtaggtg
     6121 gttgcgcagc agtcttccac gagggtgatg tagtagtcga gcatgaatcc aactgttaga
     6181 gtgtggaaca cacacatgcc ggtgccgacg ccagtgacca gtaggctttt gacgccacgg
     6241 gcgcgtagta acatatccaa gtcggtgccg atgaacgcgc tgtagcggtg tttggtgatg
     6301 atcgtttcgc cgggtcctgg ctctaagcct tcgtgaaatt cggcacccca tgtgccttcg
     6361 gcgcaggcga gcagttcggg attcttaaac tctgtccaaa ccggtgagtt ggtccactga
     6421 ttccctgaag ttttgacgaa gcagatgggg aaattttttg tccgggctgc attcacgaga
     6481 tttatcagcc gcggaatcat tttttcaacg aatgccatgt cgcggccgcg cttgcaggcg
     6541 gcaccatcgc ggtggcagaa gtcgttctgc atgtcgacga cgatcagcat gccatgcttg
     6601 ggatcgacgc gctcttccaa agaaaatgcc atggcgattg ctactcgtag agctttttga
     6661 tgaagccgct gtcgtccaac tctttgacca agctgttgtc aaaaaaactc tgtgggttgg
     6721 ctgtcttggc gcgcggattg tcccgtccat agaagtcgag aagggtggtc acgcctttca
     6781 acgaaacgta gggtgcgttt tcgaaataac cggaagtgta aagcgtgtgg gagcgttcga
     6841 gatacttggt ctgggctgga gcggtgcgta attttcttgc aagtacatca atgctctctt
     6901 tacggttttt tttgaagtag gcaatgcctt ccacataacc tttcatgaag cgcgcagcga
     6961 catcgcgatg cgcgcgcagg tagctgcgcg tggtgtcgat catgctgtgt aaataataga
     7021 tatccatgtc ggccaggtca gcgagtgtcc gatagccctg gtcatcggcg aaaaagaatg
     7081 tcggctcggt gagcacggcg gcgtcgatgc cgcctttttc cagcgccgcc atcatcgccg
     7141 gcgacgaacc gatttgcagc gtctgcacgt cgttcgcggt catgttccag ttgcgcagca
     7201 taatcagcaa caccaagtgc gatgccgcgc cgagacgggt gatgccgatt tttttaccgc
     7261 gcagatcctc gggccgtctg atgtcgccgc gggcgacgac ccgctgcacg cctttgttga
     7321 cgatggagcc gatcatgatc acgtcggcgc cgctgagcgc cgcactgatc accccgccgc
     7381 cgccaccgcc gagaaactgc acgtcgccgg cgatcagcga actcacgcca ccggcggaag
     7441 cgccgccgat caaaaccact tcgctctcga tggcgtattt tttgaacaga cctttttctt
     7501 cggcgatcca gagagcggca ttgatcggcg tgaaggaact gagaccgaca cggatgcggt
     7561 cggcggcgat tacgcattgc gccccgatca aggtaaaagc aaaaatcgcc agcaggtttg
     7621 cgaggccgtg ccgagtaagt ttcatcgagc ctcctggtta cttgtggtta gatgccgaaa
     7681 agctcacgga actctttggc gtgctgatcg gtattcaacg acacttcttt cgggatacgt
     7741 tttatcttta gccggttctg gtccattttg gcgaccagcg gttccacgtc ccggcgcgac
     7801 ggcaccaacc gtaggtcgcg aatcatctgt tggctgcgcc ggctgagggc gaagtcgacg
     7861 aaaagcctgg cgctattcgg gttggctgct ttgctgctga cgccgatgcc gtgcacggtc
     7921 gtgacgatgg gatcaaaggt gtcggaccaa tccaccggcg ctccttcttt cttcatgcgt
     7981 tcgacgcgga aagtataggc ccagcccagc ggcaattcac ccgcgccgat gagttgggcg
     8041 accagaccat gaccgcggcg ccaggttaag tcctgccgcg ccaactgctt catgtaattg
     8101 accgctcggg ttttccccca cgccagcgcg agagtgccaa accagtcgta atcagtggag
     8161 tccatgagca atttgccttt ccacttggcg tccagcaggt caggatagtt tc
//
LOCUS       DASQKS010000673         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2054624, whole genome shotgun sequence.
ACCESSION   DASQKS010000673 DASQKS010000000
VERSION     DASQKS010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054624"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VE170_17470"
     CDS             <1..692
                     /locus_tag="VE170_17470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ17287.1"
                     /translation="LVSYQPGPHGHSGGHLKVRTDEQKQWTAKLALQSLQFWLLFIAR
                     IGAASGTTVIVTHQVAHVVDVGFSKLLAASIFGFAGITSSFGRVIFGFIADRLTKQAA
                     YTLNIVMTVVGVGALMLLRDPSQAWLLYVYVIFFGIGFGSRAVIFSSLTADIFSGKGF
                     GSILGYSTVAVGVGGALGSFFGGVFHDWTGSYTMSFTLSTLLLAVSDVCIWLAAVPAV
                     ALYDQRLWARR"
     gene            815..1183
                     /locus_tag="VE170_17475"
     CDS             815..1183
                     /locus_tag="VE170_17475"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HYJ17288.1"
                     /translation="MDARLRDGIRLFNERKFFACHEILEEFYRDSEAENKAFLEGLIQ
                     LAAAFRMFVDFAEVKGAVRGIYQALIRFENYQPVFLQVRVQDLCTEAEAWAKAAEPPG
                     ATPSASNIPKIQLQRFRLFS"
     gene            1180..>3503
                     /locus_tag="VE170_17480"
     CDS             1180..>3503
                     /locus_tag="VE170_17480"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ17289.1"
                     /translation="MTAHPLSEKLAQFLRGTWTGSKRIQGIQGGARAYLLALAAADSQ
                     RPMLVIAPSANQAETLYDDLGFFLGEERGLAPLRRRLHLFPSWEVLPFENLSPHPENI
                     AGRLEGLYKLIEEPAPILIATPAALMQRVIPKAALKKSYLYIVVGQDLPREGLLDHLI
                     HWGFQNVPLVEERGDFSVRGGIVDLFPPGYGRPIRLEFDGDRLESIREFNPANQRTEA
                     VQEEFILLPMKEFALKRAGIEESLKRLEQRALELQVERRERQALLESLRDGIPVAGVE
                     FLLPFFCPDLVPVTTYLPANTLVWLDAADRVEAEGERFGQHAWERNRQAQEDHRLVAP
                     VEQLYLNEHEWRATLEPFSAIHGEALMLLAAAEKAQATTLTVESFLTSDLRQDPALHG
                     KDVSLAPLVERLKSWSNEKVIFVAPTKGDAARLRELLGGYQVEVALLDDDIPSVLARP
                     DIARGIVRGNLNQGFRLPEAQLIFVTFDEIFATRKRQPTTATKSYPSHFLSSLSELKQ
                     DDYVVHLDHGIGVYRGLKFLKVAGVEGEFLHLEYEAGDRLYLPVDRINMVQKYIGGDG
                     AQPSLDRLGGTAWEKVKAKAKKAVLAMAEELVQLYALREARGGTAFAPPDNMYKEFEA
                     AFEFEETPDQQRAIDDTLASMQSRKPMDRLVCGDVGYGKTEVAMRAAFLAVEGGKQIA
                     VLAPTTILAQQHLQTFRHRFRNHPVRIEMVSRFLTSKEIAQVLSDTAKGQVDIVVGTH
                     RLLQKDVDFKDLGLVIIDEEHRFGVVHKERLKKMR"
BASE COUNT          678 a         1002 c         1049 g          774 t
ORIGIN      
        1 tgttggtttc ctatcaaccg gggccgcacg ggcatagcgg cgggcatctt aaagtgagga
       61 cagacgagca gaagcagtgg accgccaaac tggcgttgca gagtttgcag ttctggctgt
      121 tgtttatcgc gcgcatcggc gcggcctccg gcacgaccgt catcgtcacc catcaggtcg
      181 cccatgtcgt tgacgtcggc tttagcaaat tactggcggc gtcgattttt ggtttcgccg
      241 gtatcaccag cagtttcggc cgggtaatat tcggctttat cgccgaccgc ttgaccaaac
      301 aggcggccta tacgctgaat atcgttatga ctgtcgtcgg cgtcggcgcc ttgatgttgc
      361 tgcgcgatcc gagtcaagcc tggctgctgt acgtctacgt gatttttttc ggcatcggct
      421 tcggctcgcg ggcggtgatt ttttcctcgc tcaccgccga tatcttttcc ggtaaaggct
      481 tcggttcaat cctcggttat tccacggtcg ccgtcggcgt cggcggcgca ttaggctctt
      541 tcttcggcgg cgtgttccat gactggaccg gcagctacac gatgtcgttt acgctttcca
      601 ccttgctgct ggcggtttcc gatgtctgca tctggctcgc cgcggttccg gcggtggcgc
      661 tgtacgatca gcggctatgg gcgcggcgtt aagcgccttc aagcggttcg gttaaagcta
      721 attattcagc cggtgcaatc gttggtgcaa cgcgctccga gcaactcgcc ttgagccgtt
      781 gccgagaagt tgctaccgta catttttcaa acctatggat gctcggttgc gcgacggcat
      841 acgtttattt aatgaacgga agttcttcgc ttgccacgag attctagaag agttttaccg
      901 cgacagcgag gcagagaaca aagcatttct cgaaggtttg attcagctgg cggctgcgtt
      961 tcgtatgttt gtcgacttcg ccgaggtgaa gggcgcggtg cgggggattt atcaggcgct
     1021 gattcgcttt gaaaactatc agccggtgtt tcttcaagtg cgagtgcagg atctctgtac
     1081 cgaggccgag gcttgggcta aagcggccga gccgcccgga gccacgccgt cggcatcgaa
     1141 cattccgaaa attcagctcc aacgtttccg tctcttttca tgaccgcgca cccactcagc
     1201 gaaaagcttg ctcagttcct gcgcggaacc tggacgggca gcaagcgcat tcaaggcatc
     1261 cagggcggcg cgcgcgccta tttgttggct ttagcagccg ccgacagcca gaggccgatg
     1321 ttggtgatcg cgccgagcgc caaccaggcg gaaactcttt acgacgatct gggttttttt
     1381 ctcggtgagg aacgcggttt ggcgccgttg cgccgccggc tccatctgtt tccatcctgg
     1441 gaagttttgc cctttgaaaa tctctcgccc catccggaaa acatcgccgg ccgcttggag
     1501 ggcctctaca agctaatcga agagccggcg ccgatcttga tcgctacgcc cgcggcgctc
     1561 atgcagcggg tgattccaaa agcggcgtta aaaaaatcct atctctatat tgtcgttggc
     1621 caagacttgc cacgtgaggg gttgctcgat cacctgatcc actggggatt tcaaaatgtc
     1681 ccattggtcg aggagcgcgg tgatttcagc gtgcgcggcg gcatcgtcga tctgtttccg
     1741 cccggctacg gccggccgat tcgtcttgaa ttcgacggcg accgcttgga atcgattcgc
     1801 gagtttaatc cggccaatca gcgcaccgag gcggtgcagg aagaattcat actcctgccg
     1861 atgaaggagt tcgcgctcaa gcgcgccggc atcgaggaat cgctcaaacg gctggagcag
     1921 cgcgcgctgg aattgcaagt cgaacggcgc gaacggcaag cgctgctcga atcgctccgc
     1981 gacggcatcc ccgtggccgg agtggaattc ttgctgccgt ttttctgtcc cgatttggtg
     2041 ccggtaacca cgtatttgcc ggccaacaca ctggtttggc tcgatgccgc cgaccgcgtc
     2101 gaagccgaag gcgagcgctt tggccagcat gcctgggagc gcaatcggca ggcccaagaa
     2161 gaccaccggc tggtggcgcc ggtggagcag ctctacttaa acgaacatga gtggcgtgcc
     2221 acgctcgagc cattttccgc cattcacggt gaagcattga tgctgcttgc cgccgcggag
     2281 aaggctcagg cgacgacatt gaccgtcgag tcttttctga ccagcgattt gcgccaggat
     2341 ccggcgctgc acggcaagga tgtttcattg gcgccgctcg ttgagcgttt aaaatcttgg
     2401 agcaacgaga aagtcatctt cgtcgcgccg accaagggcg atgcggcgcg tttgcgcgaa
     2461 ctgctgggcg gctaccaagt cgaagtggcg ctactcgacg atgacattcc gtcggtgctg
     2521 gcgcgtccgg atatcgcgcg cggcatcgtc cgtggcaatt tgaatcaagg cttccgcctg
     2581 cccgaagccc agctgatttt cgtcaccttc gatgagatct tcgcgacgcg caagcgccag
     2641 cccaccacgg cgaccaagag ttatccgagc cattttctct ccagcctgag cgagttgaag
     2701 caagacgatt atgtcgtcca tctcgaccat ggcatcggcg tttaccgtgg cctcaagttt
     2761 ctcaaggtgg cgggtgtcga aggcgaattc ctccatctcg agtacgaagc gggcgaccgg
     2821 ctctatttgc cggtggaccg catcaacatg gtgcagaaat acatcggcgg cgatggtgcc
     2881 cagccatcgc ttgaccggct aggcggcacg gcctgggaaa aagtcaaagc gaaggcgaaa
     2941 aaggccgtgc ttgccatggc cgaagagttg gtccagctct acgctctgcg cgaggcgcgc
     3001 ggcggcaccg ctttcgcgcc gccggacaac atgtacaaag aatttgaagc ggccttcgag
     3061 ttcgaggaaa cgccggacca gcagcgtgcc atcgacgaca cgctggcgag catgcagagc
     3121 cgcaaaccga tggaccgctt ggtctgcggt gatgtcggct atggcaaaac cgaagttgct
     3181 atgcgcgccg cttttctcgc cgtcgagggg ggcaagcaaa tcgcggtcct ggcgccgacg
     3241 acgatcttgg cgcaacagca tctgcagacc tttcgccacc gcttccgtaa tcatccggtc
     3301 cgcatcgaaa tggtcagccg gtttttgact agcaaagaaa tcgcgcaggt tctcagcgac
     3361 accgccaagg gtcaggtcga tatcgtcgtc ggcacccacc gcttactgca gaaagatgtc
     3421 gacttcaaag atttgggact ggtgatcatc gacgaagaac accgtttcgg cgtggtgcac
     3481 aaagagcggc tgaaaaaaat gcg
//
LOCUS       DASQKS010000674         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1248730, whole genome shotgun sequence.
ACCESSION   DASQKS010000674 DASQKS010000000
VERSION     DASQKS010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248730"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      213..320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            411..611
                     /gene="thiS"
                     /locus_tag="VE170_17485"
     CDS             411..611
                     /gene="thiS"
                     /locus_tag="VE170_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HYJ17290.1"
                     /translation="MKVKINGEEQEFAHSLTLSALLESLQIRPGRVVVERNREIVPRD
                     SYNATLINDGDTLEIVHFVGGG"
     gene            614..1387
                     /locus_tag="VE170_17490"
     CDS             614..1387
                     /locus_tag="VE170_17490"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HYJ17291.1"
                     /translation="MEDYFELAGTKFRSRLIVGTGKYKDFAETKKAIEVSGAEIVTVA
                     VRRVNITDPNKENLLDYLDPKKYTILPNTAGCYTADDAVRTCRLAREAGVGKLVKLEV
                     IGDDRTLFPDVPATLDAAKILVKEGFIVLPYITDDPITAQKLEDMGCAAVMPLAAPIG
                     SGLGIRNPYNIRIILEHAKVPVIVDAGVGTASDAAVVMEMGCAGVLMNTAIASAKDPI
                     LMAEAMRQGVEAGRKAYLAGRIPRKLYATASSPLDGMLN"
     gene            1397..2023
                     /gene="thiE"
                     /locus_tag="VE170_17495"
     CDS             1397..2023
                     /gene="thiE"
                     /locus_tag="VE170_17495"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYJ17292.1"
                     /translation="MPQPNFDLYLVTDRKLTHGRDLLWVLERALDGGVKAIQLREKDL
                     SGKQLFILAQRVSELCQRYQAQLFINDRVDVALAVDAAGVHLAETSMPVPIARSLLGA
                     HRIIGVSTHSVEGACKAEREGADFVVFGPVYFTPSKADYGTPQGLNALQKIVAKTSLA
                     VYAIGGIKAENLPETQKVGSRGVAVISAVMSADDPGHAAQHLLALFTR"
     gene            2028..2333
                     /locus_tag="VE170_17500"
     CDS             2028..2333
                     /locus_tag="VE170_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17293.1"
                     /translation="MAETLQQVVAYIKTNLAASLVIAFVAGFLATRLVAAERRPGVIG
                     FTIIGSIGFFLARLVIHYYQLNEYLENLQGLRFFIDLTAAFIGSFVIAGIVHFIKPT"
     gene            complement(2533..>2979)
                     /locus_tag="VE170_17505"
     CDS             complement(2533..>2979)
                     /locus_tag="VE170_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17294.1"
                     /translation="SHLLQKIALQTKKAVKGITPQAMKKLMQHDWPGNVRELENTLEY
                     AVTMADGELVTEELLLEMAVPTAVGSQESVRRGDGMALDGPLNSLKEARAEFEKAYLI
                     RLLEHCSGSAAKAAEIAGKYRADFYDLLKKHHINLQEFKHEFKKAD"
BASE COUNT          678 a          824 c          798 g          679 t
ORIGIN      
        1 gtatttttaa atcccggcga caaagtcgaa ggtgaaatcg aaggcatcgg cagtctccat
       61 gtcgacatcg tcgcgccatg attcgctgtc tggctaaaaa cagcgccgat ttttacaatt
      121 ttttcaattg aagtttccgt cgtagtatag atctaaaatc aagcgttggg cggcgcgaat
      181 ttgacttgga gctggtgctt tgttaagggt gctgtcgttg ctaggggtgt ctgccagagt
      241 cagactgaga gcctgaaacc aggtaaccct ttgaacctga tccgggtcgc accggcgtag
      301 ggaagcgtga aagtcaaagt cgcgtcgaag tcgattcggc gccacccact tgaaaccgta
      361 ttccctgacc accatcgggt ggtctgtgcg aatgcggttt ttttattgtg atgaaagtta
      421 aaattaacgg cgaagagcag gaattcgccc atagcctgac cctgagcgcg ctgttggaat
      481 cgctgcaaat tcggccggga cgtgtggtag tcgagcgcaa ccgggagatc gtcccgcgcg
      541 atagctacaa cgcgacgttg ataaacgatg gcgacacatt ggaaattgtc cacttcgtgg
      601 gcggaggatg accatggaag actattttga attggccggc acgaagtttc gttcgagact
      661 catcgtcggc accggaaaat acaaggactt cgccgaaacc aaaaaagcca tcgaggtctc
      721 gggcgctgaa atcgtcaccg tggccgtgcg acgcgtcaat attacggatc ccaacaaaga
      781 aaatcttctc gactatctcg accccaagaa atacacaatc ctacccaaca ccgccggctg
      841 ctacaccgcc gatgacgccg ttcgcacctg tcggctcgcc cgcgaagcgg gcgtgggtaa
      901 actcgtcaaa ctcgaagtga tcggtgacga cagaactctc tttccggatg tgcccgcgac
      961 tcttgatgcc gccaagatat tagtcaaaga aggctttatt gtcttgccgt acatcaccga
     1021 tgacccgatc actgcacaaa aactcgaaga catgggctgc gccgcggtaa tgccgctggc
     1081 cgcgcctatc ggctccggat tgggcattag aaatccctac aatattagaa taattttgga
     1141 gcacgccaaa gtacctgtaa tcgtcgatgc cggggtgggc acagcctcag atgcggcggt
     1201 ggttatggaa atgggctgcg ccggagtctt gatgaacacc gccattgcca gcgccaaaga
     1261 cccgatctta atggccgaag cgatgcgcca gggcgtcgaa gcaggtcgta aagcctatct
     1321 ggctgggcgt attccaagaa aactctacgc caccgcatcg agcccgctgg atggaatgct
     1381 caattagcca acgccattgc cccaaccgaa cttcgatcta tacctggtta ccgaccgcaa
     1441 gcttacccac ggccgtgatt tactctgggt gctcgagcgc gccctggacg gtggcgttaa
     1501 agcgatccaa ttacgcgaga aggatctgag cggcaagcag ctttttattc tagcgcagcg
     1561 cgtttctgaa ctctgtcagc gctatcaagc ccaattattc ataaatgacc gggtcgatgt
     1621 cgcgcttgcc gtcgatgcgg ctggggttca ccttgccgaa acttccatgc cggtgccgat
     1681 cgcgcgttca ctcttaggcg cgcaccgaat catcggtgtg tctacacact ccgtggaagg
     1741 cgcgtgcaaa gcggaacgag aaggcgccga cttcgttgtg ttcgggccag tttatttcac
     1801 accatccaaa gctgattacg gtacgccgca ggggcttaac gcgctgcaaa aaattgtcgc
     1861 gaagacttcc ctagccgttt atgctattgg aggaatcaag gcggaaaatt tgccggaaac
     1921 ccaaaaagtc ggcagccgcg gcgtggccgt gatttcggca gtcatgagcg ccgatgaccc
     1981 tggccacgcg gcgcaacacc tgttagccct gttcacgagg taaacccgtg gcagaaactc
     2041 ttcaacaagt cgtcgcctat attaaaacaa acttggccgc gagtttggtg atcgcgttcg
     2101 tcgctggttt tctggcgacg cggctcgtcg cggccgaacg ccgccctgga gttatcggat
     2161 tcacgataat cggttccatc ggtttcttcc tcgctcgctt ggtgattcac tattaccaac
     2221 ttaacgaata tctcgaaaac ctgcaggggc tgcgcttttt tattgacctg acagcggctt
     2281 tcatcggttc cttcgtcatc gccggaatag ttcacttcat taaacccaca taagcgagcg
     2341 cgccccgtgt ttaagcgccg cttcggagaa tcaagtcaga cgcgatttga caaggcgtga
     2401 aattgccggg tgctcctcac cacagccagc ttaataagag gaagcgtcag cgaaaaggac
     2461 agccgccgat ggtaattaaa accagccaga tcgcgatcgg gcatgccgag cggagccagg
     2521 agccagtggt atctagtccg cctttttgaa ctcgtgtttg aactcttgca gattgatgtg
     2581 gtgttttttc agtagatcat agaaatccgc ccggtacttc ccggcaatct ccgcggcttt
     2641 tgcggcgctg ccgctgcagt gttccagcag gcgtataaga taggccttct caaattccgc
     2701 ccgcgcttct ttgagtgagt tgagcggacc atcaagcgcc atcccatccc ctcggcggac
     2761 agactcctgg gaacccacag ccgtcggcac tgccatttcc aataacagct cttcggtgac
     2821 gagctcgccg tcggccatcg tgaccgcgta ctctaacgtg ttctcaagct cccgaacatt
     2881 gcccggccag tcatgctgca tgagcttctt catggcttgg ggcgtgatac ctttcactgc
     2941 ctttttcgtc tgcagcgcga tcttctgcag cagatggct
//
LOCUS       DASQKS010000675         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1649225, whole genome shotgun sequence.
ACCESSION   DASQKS010000675 DASQKS010000000
VERSION     DASQKS010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649225"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VE170_17510"
     CDS             <1..206
                     /locus_tag="VE170_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17295.1"
                     /translation="NIVMITTTLPDRPCTEDRSLIGRHVAVLVIDQSAVDFLPGLLIL
                     SYTFPAAGRKKGERANARHYDGV"
     gene            complement(191..859)
                     /locus_tag="VE170_17515"
     CDS             complement(191..859)
                     /locus_tag="VE170_17515"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HYJ17296.1"
                     /translation="MAETVQYRRNGLTDAAQFRNWCAHCRRPKVGCYCALIEPFESLP
                     RFVILSQPREAKHRLGTGRMAHLGLGNSLWFEGVDFSEHSQVNREIADPGNAPMLLFP
                     SRHAVNLSNITVTERQQLTACSRTPVVFVLDGTWKSVRKMMRLSDNLRRLPTICFKPL
                     TPSAYGFRREPRPECYSTIEAIHHVIELFNDGDAAEARPRRHDRLLKAFHFMVARQLA
                     YTPS"
     gene            complement(865..1455)
                     /locus_tag="VE170_17520"
     CDS             complement(865..1455)
                     /locus_tag="VE170_17520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ17297.1"
                     /translation="MSTDDRIRWDRQHGSSRDAEPPAAFLSMILESDAWPALSGNGLD
                     IACGKGRNAIYLAQRGFAVTALDISAIALAEGRRRARQQDLLIDWHRCDLESSAPAFA
                     AGDFDLIVNFNYLQRALIRPMQQALKPGGHVIFETFLIDQAAIGHPTNPDYLLRHNEL
                     LECFDDDFRVLFYREGKFADGGTEAFRAAIFAQRID"
     gene            complement(1472..2233)
                     /locus_tag="VE170_17525"
     CDS             complement(1472..2233)
                     /locus_tag="VE170_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HYJ17298.1"
                     /translation="MELSATARLPERVDWALDWLYPPRCRACRGRILGRDADYFCSRC
                     RDQIQLVGHPLCNICGRPYPDGSGDDHACGICLERRPQFVAARAWACYPRADDHPLRQ
                     VVQKFKYNRKVSLGKPLGRLMARGCQEFLAAVNADVIVPVPLYPKRLRWRGFNQSVLL
                     AREVGRAYNIQLDRFLLIRHRETAAQTQLPEEARRRNVRGAFALNPERPVKNKRILLV
                     DDVYTSGATVDECSRTLKRGGAKEVYVLTLARAVY"
     gene            complement(2292..3836)
                     /gene="gpmI"
                     /locus_tag="VE170_17530"
     CDS             complement(2292..3836)
                     /gene="gpmI"
                     /locus_tag="VE170_17530"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HYJ17299.1"
                     /translation="MSTTQPVILVILDGFGINPRKEYNAIANAATPNLEALLREYPNS
                     RLSMSGVDVGLPAGQMGNSEVGHMILGAGRVVYQDLTLIHKDIDEGEFYRNRVLLDAL
                     GKTKATSDRLHLMGLLGDGGVHSHQRHLEALLEMAKREQLAQVYLHLFLDGRDTPPNS
                     AEQFILDLNEKLKNFPNVKVATLCGRYYAMDRDKRWDRVEKAYNCLTAGMGNHTDSAL
                     AAIRASYQAKITDEFVLPTVIDGVVPDGLIRDGDGVIFFNFRADRARELTRAFIDAEF
                     TEFSRPRRLNLSTFTTMTRYDETLKVPVAYQPREIRKILGELASRAGVKQLRIAETEK
                     YAHVTYFFNGGEESKFPGEERILIPSPKDVATYDLKPEMSARPVTEALVKHLRETDVG
                     LVIANYANADMVGHTGNFEASVKAVEVIDECLGKVVDATLAKHGKVIITADHGNIEQL
                     IDYDTGMPHTAHTINLVPVILIDEAHRRCRLNEGTAIDVAPTVLQLLGLSQPAEMTGH
                     SLIVES"
     gene            complement(3868..4302)
                     /gene="rsfS"
                     /locus_tag="VE170_17535"
     CDS             complement(3868..4302)
                     /gene="rsfS"
                     /locus_tag="VE170_17535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYJ17300.1"
                     /translation="MKVKALSAWDKALLLTRAALDKKAGDLVVRDVHAHTSIAEYFIL
                     CSGRSDRQVQSIAQGLQQEAASAGVRPYAVEGTQRGHWVLMDFSDVIVHIFYEPMRQF
                     YDLDGLWSHAPQAKLPASYANLIAQQRASDAQMSDSPSSHFD"
     gene            complement(4299..>4859)
                     /gene="nadD"
                     /locus_tag="VE170_17540"
     CDS             complement(4299..>4859)
                     /gene="nadD"
                     /locus_tag="VE170_17540"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HYJ17301.1"
                     /translation="FIPASIPPHKRGKTTTPAKDRLRMVRLAVARNPRFAVSTVELDR
                     PGPSYSVDTIREFARQLSKRDSLFFIIGLDAFIEIRTWKDFKEIFPLCDFIVTSRPGS
                     KENDPLKGAGIAVKKLFCYDSKKRNYRHQSGTRVHFIELTDIAISASEIRALVRRGKS
                     VRYLLPSAVKNYIKRRRLYADRRESR"
BASE COUNT         1017 a         1423 c         1430 g          989 t
ORIGIN      
        1 cgaatattgt gatgataaca acaacgctgc ccgatcggcc ctgcaccgag gacaggtcat
       61 tgatagggcg ccatgtcgca gttttagtaa ttgatcagtc cgccgtcgac ttcctaccgg
      121 gactattgat attatcttat actttccccg cggccggtag aaaaaagggg gaacgcgcca
      181 atgcaagaca ttatgacggc gtgtaggcga gttgccgggc gaccatgaag tgaaaggctt
      241 taagcaggcg gtcgtgacgg cgcggccgcg cttcggcggc gtccccgtca ttgaacagtt
      301 cgatcacgtg atgaatcgct tcgatggtcg agtagcactc ggggcgcggt tcgcggcgaa
      361 agccataggc cgacggcgtc agcggcttga agcaaatcgt cggcagccgg cgcagattgt
      421 cactcaggcg catcatctta cgcacgctct tccaagtgcc gtcgagaacg aaaaccaccg
      481 gtgtccgcga gcacgccgtc agctgctggc gctcggtcac cgtaatattc gaaagattca
      541 cggcatggcg gctcgggaac agcagcatgg gcgcgttgcc gggatcggca atctcgcggt
      601 tgacctgaga atgctcggag aaatccaccc cctcgaacca aagcgagttt cccagcccga
      661 gatgcgccat ccggccggtg ccgagccggt gcttggcctc gcgcggctgg ctgaggatga
      721 cgaagcgcgg cagcgactcg aacggttcga tgagcgcaca gtagcagccc accttgggcc
      781 gccggcaatg cgcgcaccag ttgcggaact gcgccgcatc ggtcaagccg tttcgtctat
      841 attgaactgt ttcagccacg atgttcagtc aattctctgg gcgaaaatgg cggcgcgaaa
      901 agcttccgtg ccgccatcgg cgaatttccc ttcgcggtag aacaaaacgc gaaagtcatc
      961 atcaaagcat tcgagcaatt cgttatgtcg taacaggtaa tcgggattcg tgggatggcc
     1021 aatcgccgct tgatcgatca gaaaagtctc gaagatcaca tggccgccgg gcttcagcgc
     1081 ctgctgcatg ggccggataa gcgcgcgctg aaggtaattg aaattaacaa tcagatcgaa
     1141 atcgccggca gcaaacgccg gcgcgctcga ttccaaatcg cagcgatgcc agtcgatgag
     1201 cagatcttgc tgccgcgccc gccgccggcc ttccgccaaa gcaatcgcag aaatatccag
     1261 cgccgtcacg gcaaagccgc gctgcgcgag ataaatggca ttacgcccct tgccgcaagc
     1321 gatgtccaaa ccgttgccgc taagcgccgg ccaggcgtcg ctttccagta tcatgctcag
     1381 aaaagccgcg ggtggttccg cgtcacgcga actcccatgc tggcggtccc agcgaattcg
     1441 gtcgtcggtg gacatcgctt gttcagatcg actagtacac cgcccgcgct aaagtcagga
     1501 catacacttc tttggcgccg cctcgtttca acgtgcggct gcattcgtcg acggtggctc
     1561 ccgaggtgta aacatcgtca accagcaaga tgcgcttgtt tttgaccggt cgctcggggt
     1621 taagcgcgaa agcaccgcgc acgttacgcc gccgcgcttc ctccggcagc tgagtctgcg
     1681 ccgcggtttc acggtggcgg atcaagagga accggtccaa ctgaatattg taggcacggc
     1741 cgacttcgcg ggccaagagc accgattggt tgaagccgcg ccagcgcagt cttttcggat
     1801 aaagcggcac cggcacgatc acgtcggcgt taaccgccgc aaggaattct tggcagccgc
     1861 gcgccatcaa tcgccccagg ggtttgccca gtgaaacttt ccggttgtat ttgaactttt
     1921 gcaccacctg gcgcagtgga tgatcgtccg cgcgagggta gcaagcccag gcgcgcgccg
     1981 cgacgaactg cggtcgccgt tctaaacaaa taccgcaagc atgatcgtcg ccactgccgt
     2041 cgggataggg ccggccgcaa atattgcaca gcggatggcc gaccagttga atttggtccc
     2101 ggcagcgcga acaaaaatag tccgcatcac gaccgagaat tcggccccgg caggcccggc
     2161 agcgcggcgg atagagccag tcgagcgccc aatcgacacg ctccggcaat cttgcagtag
     2221 cggaaagctc catggcaaac gagaacgtag caaaacgcgg gcacaggaag caaagccaag
     2281 atgacaggcg cctaagactc gacgattagc gaatgaccgg tcatctctgc cggctgcgac
     2341 aaacccagca gctgcagcac cgtgggcgca acgtcgatgg ccgtgccttc gttaagccgg
     2401 cagcgccgat gggcttcgtc gattagaatc accggcacta aattgatcgt gtgcgccgtg
     2461 tgcggcatgc cggtatcgta gtcgatcaac tgctcgatgt tgccatgatc ggcggtgatg
     2521 atgaccttgc cgtgcttggc caaagtcgcg tccaccactt tgcctagaca ctcgtcgatc
     2581 acttccaccg ctttgaccga ggcttcgaag ttgccggtgt ggccgaccat gtcggcattt
     2641 gcgtaattgg cgatcaccag gccgacgtcg gtttcgcgca agtgtttcac cagcgcctcg
     2701 gtcaccggcc ttgcgctcat ctccggtttc aaatcgtagg tcgccacgtc tttcggcgaa
     2761 ggaatgagaa tccgttcttc gccggggaat ttactctcct cgccgccgtt gaagaagtaa
     2821 gtcacatggg cgtacttttc agtctcggcg atacgcaatt gctttacccc cgcccggctg
     2881 gcgagttcac ccaaaatttt tctaatctcg cgtggctggt aagccaccgg aactttaagg
     2941 gtctcatcat accgggtcat ggtcgtgaag gtggacagat tgaggcgccg cggccgcgaa
     3001 aactccgtga actcggcatc gatgaaggcg cgcgtcagct cccgcgcccg gtcggcgcgg
     3061 aaattaaaaa atatcacacc gtcgccgtcg cggatcagtc catccggcac gaccccgtca
     3121 atgaccgtcg gcagcacaaa ttcatctgtg atcttcgcct gatagctcgc gcgaatcgcc
     3181 gccagcgcgg agtccgtgtg gttgcccatg ccggcggtca gacaattgta ggctttctcg
     3241 acccggtccc agcgcttgtc gcggtccatg gcatagtagc gaccgcacaa cgtggcgact
     3301 ttgacgttgg ggaagttttt taatttctca ttgaggtcga ggataaactg ctccgcgcta
     3361 ttgggcgggg tatcgcggcc atcgaggaac agatggagat agacttgcgc caattgttcg
     3421 cgcttggcca tttcgaggag cgcttccagg tggcgctggt gactatgcac gccgccgtcg
     3481 cctaacagtc ccatcaagtg caagcgatca ctcgtggcct tggtcttacc tagcgcgtct
     3541 agcagcacgc gattccgata aaactcgccc tcgtcgatgt ctttgtggat cagggtcaaa
     3601 tcctggtaga cgacgcgccc cgcaccgaga atcatgtggc ccacttccga gttgcccatc
     3661 tgccccgccg gcaggccgac atctacaccg gacatggaca ggcgcgagtt ggggtattcg
     3721 cgcagtaaag cctcgaggtt gggcgtagcc gcattggcga tggcgttgta ctctttgcgc
     3781 ggattgatgc cgaagccgtc aagaatgacc aatatcaccg gctgggttgt gctcacgagg
     3841 attcctttct gagttgcacc ggagagatta atcgaaatgg ctgctcggtg agtcggacat
     3901 ttgggcatcg ctggcgcgct gctgcgcgat cagattggcg tagctggccg gcagcttcgc
     3961 ctgcggcgcg tgactccata gaccgtcgag atcgtagaac tggcgcatcg gctcgtagaa
     4021 gatgtggaca ataacgtcgg agaaatccat caacacccaa tgaccgcgtt gcgtgccttc
     4081 gacggcatag ggccgtacgc cggcgctcgc cgcctcttgt tgcaagcctt gggcgatgct
     4141 ctgcacctgg cggtcggagc ggccagagca aaggatgaag tattcggcga tcgacgtgtg
     4201 cgcatgaacg tcgcgcacga ccaggtcgcc ggccttctta tccagcgccg cccgcgtcag
     4261 tagcagcgct ttgtcccagg cgctcagtgc tttaactttc accgactctc cctgcggtcg
     4321 gcgtatagcc gacgccgttt aatatagttc ttcaccgctg agggtagcaa ataacgtaca
     4381 gactttcctc tgcgcacaag cgcgcgaatt tctgaagcgg agatggcgat atcggtgagc
     4441 tcgataaaat gcactcgcgt gccgctctga tgacggtaat tgcgcttttt tgaatcataa
     4501 caaaacagtt ttttcacggc aatgccggcg cctttgagcg gatcgttctc tttgctgccc
     4561 ggtcgtgacg tgacgatgaa gtcgcacaaa ggaaaaattt ctttgaaatc tttccaggtc
     4621 ctgatctcga tgaacgcgtc caagccgatg ataaaaaaaa gcgaatcgcg cttgctgagc
     4681 tgcctagcaa attcacgaat cgtgtcgacg gaataagacg gcccggggcg gtctaactcc
     4741 acggtcgaaa ccgcgaagcg cggattgcgc gccaccgcca ggcggaccat gcgtagccgg
     4801 tccttggccg gcgtcgtggt cttgccgcgc ttgtgcggtg gtatcgaagc cgggatgaa
//
LOCUS       DASQKS010000676         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2518292, whole genome shotgun sequence.
ACCESSION   DASQKS010000676 DASQKS010000000
VERSION     DASQKS010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2518292"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VE170_17545"
     CDS             complement(<1..221)
                     /locus_tag="VE170_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ17302.1"
                     /translation="MLFGSCLCGGVKYEIDGALGAVTNCHCSLCRKMSGSAFASGAAI
                     PAATFRFTAGQELLKEWQSSPGNHRVFCGR"
     gene            complement(281..1246)
                     /locus_tag="VE170_17550"
     CDS             complement(281..1246)
                     /locus_tag="VE170_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17303.1"
                     /translation="MNRGKNRPFLSRAPLVVFALMILLAGSPAPAAERVTIATPSRGL
                     FEFPVVVAMRKGFYRDEGIEVDKIQMQPAVGVKALISGDVDYLLAWGSALRAAVSGVP
                     IKAVLGFAGRPLHVLIVRPEIKSPKDLKGKVIGVDSVAGTVDYLSRVAVRHFGFEPEK
                     DVKIIVTGESPTRLAALRAGAIDATPIDVAFAVKAEDEGFKRLVYLGDLIELPLSGIA
                     VMEQKLQTQRDQIKRVVRAGVRGLRFMKQNRNETVQMLADYLRITPSQAARAYDASIN
                     SFTDDGIISDKGVLLDVQLTKERLKLTKDIPLSQLVDWSVVREVK"
     gene            complement(1264..2088)
                     /locus_tag="VE170_17555"
     CDS             complement(1264..2088)
                     /locus_tag="VE170_17555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ17304.1"
                     /translation="MAAKKSTGISATMIKFGGGIGAARAFPKAKGKRPAVIILHERYG
                     VDQHTKDLTARLAQAGFVGLAPDLFHRFTGDRQALLRGEQRVDLTDDGALADLNAAVE
                     YLKRLKQVDGSRIAIIGVCQTGRQPLLLAAKRNDIAGAVVLYGAVGGREWQGNDLRPT
                     PISELVAQVNCPVLGIFGEADHIISVDDVARFRDCFEKSKKSCHIRIYRDAPHGWLND
                     TMPGRYRKEPANDAWKLMMAFLKKCFAGGWDRDRIFCTYESDYAANYDFSRNVRME"
     gene            2220..2537
                     /locus_tag="VE170_17560"
     CDS             2220..2537
                     /locus_tag="VE170_17560"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HYJ17305.1"
                     /translation="MNLTDGSTFVGDLTTIGRKSGRPRKVELRFLFYQGNFYATSTRV
                     QGKHWCQNMIKNNAVDVTVKSVKFACTAKQVTDAALRTQVLTLRDSPPQLERVVFEIA
                     PNK"
BASE COUNT          629 a          928 c          833 g          630 t
ORIGIN      
        1 cggccgcaaa atacccgatg attgcccggc gacgattgcc attccttgag cagctcttgt
       61 cccgccgtga agcgaaaggt cgccgccggg atcgccgccc cgctggcaaa agccgagccc
      121 gacatcttgc ggcacaagct gcagtgacag ttggtcaccg cgccgagtgc gccgtcgatc
      181 tcatatttca caccgccgca aagacaggat ccaaacagca tggaagctcc gatcaggtta
      241 actcgaaaaa cgatatggag acgtttaact taaaagagta ctacttcact tcccgcacca
      301 cactccaatc gaccaattgg ctgagcggaa tatctttggt cagcttgagc cgttccttgg
      361 tcagctgcac gtcgaggagc acgcctttgt cggagatgat gccgtcgtcg gtaaaagaat
      421 tgatcgacgc gtcgtaggct cgggcggcct gggagggcgt gatgcgcagg taatcggcaa
      481 gcatctgcac tgtctcattg cgattttgtt tcatgaaccg caggccgcgc acgccggcgc
      541 gcacgactcg cttgatttga tcgcgctggg tttgcagttt ctgctccatc acggcgatac
      601 cggaaagcgg caattcaatg aggtcgccca aataaaccag ccgcttgaac ccctcgtcct
      661 cggctttgac cgcgaaggcg acgtcaatgg gagtagcgtc gatggcgccg gcgcgcaacg
      721 ccgccaggcg cgtcgggctc tcgccggtga cgatgatctt gacatctttc tccggctcga
      781 atccgaagtg gcgcaccgcc acgcgcgaca gatagtcgac cgtgccggca acggaatcga
      841 caccgatgac tttgcctttc aaatctttgg gcgacttgat ctccggccgg acgatcaaga
      901 catggagagg ccggccggca aaacccaaca ccgctttgat cggcacgccg cttaccgctg
      961 cgcgcagcgc cgaaccccat gcgagaagat aatcgacgtc gccggagatt aatgccttga
     1021 ccccaacggc cggctgcatt tgaatcttat cgacctcgat cccttcgtct ctgtaaaatc
     1081 ccttgcgcat ggcgacgacc acgggaaatt cgaacagccc gcggctcggt gtggcgatgg
     1141 tcacgcgctc cgccgccggc gctgggctgc cggcgagcaa aatcatcagg gcgaagacga
     1201 caagcggcgc ccgtgagagg aatgggcggt tttttcctcg attcattttt tgcaacctct
     1261 ccgctattcc atgcgaacgt tcctgctgaa atcgtaattg gccgcgtaat cgctttcgta
     1321 ggtgcagaaa atccgatccc tatcccaccc accggcaaaa cattttttca gaaatgccat
     1381 catcagcttc caagcgtcgt tggcgggctc tttgcgataa cgtcccggca tggtgtcgtt
     1441 gagccaacca tgaggcgcgt ctcggtagat tcggatgtgg caactcttct tggatttctc
     1501 gaaacagtcg cggaagcgcg cgacatcgtc gacggagata atatgatccg cctcgccgaa
     1561 aatccccagg accgggcagt tcacctgggc gaccaactcg cttatcggag taggccgcag
     1621 gtcgttgcct tgccactcac ggccaccgac cgcgccgtag agcaccacgg caccggcgat
     1681 gtcgttgcgc ttggccgcca gcagcagcgg ctgacggcca gtttggcaga cgccaataat
     1741 ggcgatccgc gagccgtcaa cttgtttcag tcttttgaga tattccaccg ccgcgttcag
     1801 atctgccagc gcgccatcgt cggtgaggtc aacgcgctgc tcgccgcgca atagagcttg
     1861 gcggtcgccg gtgaagcgat gaaacaggtc cggtgccaag ccgacaaagc ccgcttgggc
     1921 cagcctggcg gttagatctt tggtatgctg gtcgacccca tagcgctcgt gaagaatgat
     1981 caccgccgga cgtttgcctt tggctttggg gaaagcccgc gcggcgccga tgccgccgcc
     2041 gaacttgatc atcgtcgcgc tgattcccgt agatttcttg gctgccatca cgtctccact
     2101 tcccttggcg ttaggattta aaaaatgtta accggcgcca agcttatccc ccgatccgcg
     2161 agggagtcaa ggcggcggga agcttgcgac gcgacgacct tgaacgtata ttcccgccca
     2221 tgaatctcac agacggttcc actttcgtcg gagacctcac caccatcggc cgcaaaagtg
     2281 gccgaccacg caaagtggag ctgcgttttc tcttctatca aggaaatttc tacgccacct
     2341 cgaccagagt gcaaggtaaa cactggtgtc agaacatgat caagaacaac gccgtcgatg
     2401 tcacggtgaa aagcgtgaag ttcgcctgca ccgccaagca ggtgaccgat gccgcgctgc
     2461 gcacgcaggt cttgaccctg cgcgactcgc cgccgcagtt ggagcgcgta gttttcgaaa
     2521 ttgcccccaa caaataaccg gatcttcgcc gggatcttgc gaacagaatt tcacggcaaa
     2581 actatagacc ggcagataac gccagacagc gctggtgcga aataaaccgc gtgacccgaa
     2641 gtcgctttcg acgcgccgat ctgtcgatgc cctggcaaaa aaccttgcct aagcccgcgc
     2701 cgctggagcc cagtcaaggc aacgtccggg tgaaatggct ctgcccgtcg cgccggcaga
     2761 tccgaccatc cgattaggag aaacggtgaa ccgctctgct cgacctgctt agcggtgttg
     2821 aatggttttt ttcagcgccc gcgctcaagg cagcgaggcg atgccggttt gcagttccga
     2881 ccgaactcgg acgaagtgat tctacttttt cgactcttcc ttcgcctcgc tcgacttctt
     2941 ttcgacggtc tcaactccct tgaccatgcc ttccatcatc ttctgttctt tctgcgcgcc
     3001 ttgttggatc gcctgcttgg
//
LOCUS       DASQKS010000677         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3768101, whole genome shotgun sequence.
ACCESSION   DASQKS010000677 DASQKS010000000
VERSION     DASQKS010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768101"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="VE170_17565"
     CDS             <1..1872
                     /locus_tag="VE170_17565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ17306.1"
                     /translation="FMTTQPTVLIVNDDPLQLRIAATILRRDGFIVLSCPSAEEALRL
                     LSEPSVVNAIVTDLYMPGIDGWRFCRLLRSAAFAQFNRIPILVVSAIFSGADAEELTV
                     QLGADGFLAAPYEPATLCRAVRGLLVDSKPISSKTVLLVEPDAAFAEDLAAIFASEGY
                     RVAHATDGAQALSRFSHEPSQIVVLDGDHAEVMTPGLIETIKQPGTPTAVIVMTSVNS
                     ADLAMDLLRKGADNQLQKSAVRQELLQLCESAVRQRALLRIEELLQIRTQRLRDSEER
                     YRDLFENAGNGIATYTLDGVIVSVNGALENLLAASRDVLAGKVYSRLMTAASFTDAKK
                     AQEDARTGNQDSWVCGLELARSDSTVVPVESHCRFLRGKGGVPGLVMATYRDLTAERL
                     LERQRAEFTAMLAHDIRNPVGLICGYAEFLLRDQGVPMDAAMARKCNERIFNAAQVIE
                     SLVSNYLDFARIEAGRLDLSKRRFELVGLLRRIVERFEGVAQMRGIRFQLHAQPTGKF
                     VDGDTLALDRVIANLLNNAFKFTPDNGVISLSVMGCAGDAVIKVCDSGPGIAPERLPT
                     LFQKFNRIELADRQEGVGLGLYIVSELVGAHGGKVEVASTPGLGTCFSVVLPLADEA"
     gene            2029..2439
                     /locus_tag="VE170_17570"
     CDS             2029..2439
                     /locus_tag="VE170_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYJ17307.1"
                     /translation="MEQQATENTIRDKARKWAAEYGEDFVLELAGDFLRDAEARMVRL
                     RQALERGDGDGLAFEAHTLKSSAANMGALAFADLAKRFEVAGRNGDLAAVAGDVNCFE
                     RQFAAVKASLEKMLSMPSKFLSEERSRRESSSGL"
     gene            2701..3693
                     /locus_tag="VE170_17575"
     CDS             2701..3693
                     /locus_tag="VE170_17575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYJ17308.1"
                     /translation="MKILIADDASTMRHLLLSALSRIGYDVEVAASGDHAWSVLQRSE
                     APEIAILDWMMPGMTGVEICQKLRERTERNDGPYVYVIMLTGLDAASDLAAGLDAGAD
                     DYMTKPFKPAELNARLRNAERILALQRELSVVQKELRTAAQRDPLTGLANRQAIRTSL
                     DDAVAQAHRQQQSLGVILFDIDDFKMLNDTRGEAEGDVILQRVATRLTESIGPDDRVG
                     RFGAGRFMIVIPAGNLYETVMLAERIRKLLCDSPIDAGCEPISISASFGVSAASSEPD
                     KAAKPLIEAARRALHRAKAAGGNRVYLAASDPQAVSIGENQTLVAETAQPRTAI"
BASE COUNT          836 a         1080 c         1185 g          830 t
ORIGIN      
        1 tttatgacaa cccaaccgac ggtactgatc gtcaacgacg acccgctcca gttacgcatt
       61 gccgcgacga tcctgcggcg tgacggtttt attgtgctct cttgcccgag cgccgaggag
      121 gcgttgcgtc tgttgtccga gcctagtgtt gtcaatgcca ttgtcacaga cctgtatatg
      181 ccgggcatcg atggctggcg tttctgccgc cttctccgct cagctgcctt tgcccagttc
      241 aatcgcatcc ctattctcgt cgtgtcggcg atattttccg gtgccgacgc tgaggaacta
      301 accgtccaat tgggcgccga cggctttctc gccgcgccct acgagccggc gacgctgtgc
      361 cgcgcggtgc gcggcttgtt ggtcgacagt aaaccgattt catcgaagac cgtgctgctg
      421 gtcgaacccg atgccgcctt tgcagaagac ctggcggcga ttttcgcgtc cgagggttat
      481 cgcgtggccc atgcgacgga cggcgcccag gcgctgtccc ggttcagtca cgagccgagt
      541 cagatcgtcg tacttgatgg cgatcacgcc gaggtcatga cgccagggct gatcgaaacg
      601 atcaagcagc cgggtacgcc gacggcggtg attgtcatga cctcggtgaa ctctgccgac
      661 ctggccatgg atctgctgcg caaaggcgcg gacaaccagc tgcaaaaatc tgcggtgcgg
      721 caagagctct tgcagctttg cgaaagcgcg gtacgccaac gggcgctgtt gcgcatcgaa
      781 gagctattgc aaatccgcac ccagcgattg cgcgacagcg aagaacgcta tcgcgacctg
      841 ttcgaaaacg ccggcaatgg catcgccacg tacaccctcg acggcgttat cgtcagcgtc
      901 aatggcgcgc ttgaaaacct cttggcagcg agccgcgacg ttttggccgg caaggtctac
      961 agcaggctga tgaccgcggc gtcgttcacc gatgcaaaaa aagcccaaga agacgcccga
     1021 accggcaacc aggattcttg ggtctgcggc ttggaattgg cgcgttccga tagcaccgtc
     1081 gttccggttg aaagtcactg tcgattccta cgcggcaagg gtggcgtgcc ggggctggtc
     1141 atggcgacct accgggatct cacagcggag cgcctactgg aacggcagcg cgccgaattt
     1201 accgccatgc tggcgcacga tatccgcaat ccggttggct tgatttgcgg ttacgccgaa
     1261 ttcttgttgc gcgaccaggg tgtgccgatg gatgcggcaa tggcccgcaa gtgtaacgaa
     1321 cgtattttca acgccgcgca ggtcatcgag tcactggtga gcaattattt agatttcgcc
     1381 cgcatcgaag cgggccgcct cgatctttcc aagcggcgct tcgaactggt tgggctgctc
     1441 cggcgcatcg tcgagcgctt cgagggcgtc gcgcaaatgc gcggcattcg gttccagtta
     1501 cacgcgcaac ccactggcaa attcgtcgac ggcgacactt tggcattgga ccgggtgatc
     1561 gccaatctgc tcaacaatgc cttcaagttt actcctgaca atggcgtcat cagcctgagc
     1621 gtcatgggct gcgccggcga cgcggtaatc aaagtttgcg atagcggacc aggaattgcc
     1681 ccggagaggt tgccgacctt gttccaaaaa tttaaccgca tcgagctcgc ggatcgccag
     1741 gaaggtgtcg gtttgggact gtatatcgtc agcgagttag tcggcgccca tggcggcaag
     1801 gtggaagttg ccagcacgcc aggtttggga acttgttttt cagtggtctt gccgctggcg
     1861 gatgaggcgt gagtgaacgt gggaaaattc atgcacgaaa tcctcaagaa attcgaaatt
     1921 aaccacacta ttctggccac tccgggttcg ccgtcccggt tttggaatga cgcgggtttg
     1981 ggcttagcga tataggcttc cggattttca acagcggagg attcggcgat ggaacagcag
     2041 gcgactgaaa atactattcg tgacaaggct agaaaatggg ccgctgagta cggcgaggat
     2101 tttgtcctgg aattagccgg ggattttttg cgcgacgcgg aggcgcgaat ggtgcgtctg
     2161 cgccaagcgt tggagcgcgg cgatggggat ggcctcgctt ttgaagcgca tacgctcaag
     2221 tcgagcgccg ccaatatggg cgcgctggct tttgccgatc tggcgaaacg attcgaagtc
     2281 gctggccgca acggcgattt ggcagcggta gccggcgatg tcaattgctt cgaacggcaa
     2341 tttgccgccg tcaaagcaag tttggaaaag atgctcagta tgccgagcaa atttctcagt
     2401 gaggaaagat cgcggcgcga atcaagctcc ggattgtagg gctcacgacg aagacataat
     2461 acggtcagac tcactacagg ctccgggtac gaaggttatc gtagggcttg taccggtcat
     2521 tcgcgaattt ttgcgcaaaa gcgcaaagac cgacaaggtc ggaagaaatt aaccgtacgg
     2581 tttgcaagac tatcgatgtc tcgtttttaa accggcatac cttgcgtttc ttcgacgata
     2641 ctcaagatag gcaggatgag agtagatctg gtgacttcca accgcagtaa ggaatagcaa
     2701 atgaaaatac tcattgccga cgatgcctcg acaatgcgcc acctgttgtt atcggcattg
     2761 agccggatcg gttacgacgt tgaggtcgcg gccagcggcg accatgcctg gtcagtattg
     2821 cagaggtctg aggcaccaga aatcgccatc ctagattgga tgatgcccgg aatgacggga
     2881 gtcgaaatct gtcaaaaact gcgcgagcgt actgagcgca acgacgggcc ctatgtctat
     2941 gtcatcatgt tgacaggctt agatgcggcc agcgatctcg cggctggcct tgatgccggc
     3001 gccgacgact acatgacgaa accttttaag ccggcagaat tgaacgcccg gctgcgcaat
     3061 gcagagcgga ttttggcgtt gcagcgtgag ttgtcggtgg tccaaaaaga gcttcgcacc
     3121 gcggcgcagc gtgacccgtt gacgggtttg gcgaaccgtc aagcgatccg caccagcctc
     3181 gatgacgcgg tggcacaggc ccatcggcaa cagcaatccc tcggtgtcat actgttcgac
     3241 attgacgatt tcaaaatgct taacgacaca aggggtgagg ccgaaggcga cgtaatctta
     3301 caacgggtag ccacccgctt gaccgaatct atcggtcccg atgatcgggt cggccgtttc
     3361 ggggccggcc gatttatgat cgtgataccc gctggtaatc tgtacgaaac ggtcatgctc
     3421 gccgaacgta tccgtaagct gctctgcgac tcgccgatcg atgccggctg tgagccgatt
     3481 tccatcagcg cgagttttgg tgtcagcgct gcttcgtcgg agccagataa agccgcgaaa
     3541 cctctcattg aagccgcccg acgggccctc catcgcgcca aagcggcggg cggcaaccgg
     3601 gtttacctgg cggcgtctga tccccaggct gtgagtattg gcgaaaacca aactctcgtg
     3661 gccgagaccg cccaaccaag gacagcaata tgagaagcga gcggcagcgg gggttgttgc
     3721 atgagcgaac cagcgactca tgccgctgaa gctgagctgc gcccacgatt aaacaagatt
     3781 cgcgtctcac aggaagttgg tcaactgatt ctcgcagcgc aagactccaa ggacttctcg
     3841 acccagtgct gcaaccaatc accgaggcct gtagcttcga tcttggagcg attctagtgt
     3901 ccgattcatg gggaggcggg gggttttgta g
//
LOCUS       DASQKS010000678         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4181832, whole genome shotgun sequence.
ACCESSION   DASQKS010000678 DASQKS010000000
VERSION     DASQKS010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4181832"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VE170_17580"
     CDS             <1..374
                     /locus_tag="VE170_17580"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ17309.1"
                     /translation="MGGRIGIHFAAATPSVRAFVGYYPSVRDEEPSKLRPRHPNDAVK
                     DFKCPSQIFLGGNDHVASIPVQQKLMASFQANGQPLDWHFFQRAGHGFAMADGDCYDP
                     ELAEQAWGLTANFLRTELDNR"
     gene            complement(388..858)
                     /locus_tag="VE170_17585"
     CDS             complement(388..858)
                     /locus_tag="VE170_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ17310.1"
                     /translation="MEITIRKAVIDDAAQLAKLLQEIGWFEAFSSRDLKDSIARVEGR
                     LRQCLADDSHSTYVAESPHGMITGYGSVHWLPYLFMAGPEGYVSELFVRDDMRGQGVG
                     RQLLKIIEIESRGRSCQRLSLINLRNRESYRRQFYLKAGWHERSDAANFVYTIA"
     gene            complement(956..2134)
                     /locus_tag="VE170_17590"
     CDS             complement(956..2134)
                     /locus_tag="VE170_17590"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ17311.1"
                     /translation="MIDYDVAVIGAGAAGLMCAIEAGKRGRRVLVIEHCDRVGKKILI
                     SGGGRCNFTNLDVTPENFLSANPHFCKSALARYTQWDFIALVERHGIAYHEKERGQLF
                     CDQSARQVVKLLTDECHEAGVQIATDCTVNSVERGAAGFVVKSKLGSSGAASLVVACG
                     GLSVPKMGASGYGYELAKRFGHAIVPMRAGLAPLVFSPDDLVRYKDLAGVSLAVQVEC
                     RRQRFANAMLFTHRGVSGPAILQISSYWQPGDDLMINLMPLTDADAWLKGRQSARPDV
                     ELKTVLGEFLPKRLAQRLCDIDLGSRPLRQYSARDMRGICERLQSWRFRPGETEGYRT
                     AEVTLGGVDTGELSSTTMESKIVSGLYFIGEVVDVTGHLGGFNFQWAWSSGWAAGQVV
                     "
     gene            complement(2573..2797)
                     /locus_tag="VE170_17595"
     CDS             complement(2573..2797)
                     /locus_tag="VE170_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYJ17312.1"
                     /translation="MSLPEPLITLSPDRMSGAPVFAGTRVPVQTLIEYLEANDSLNDF
                     LEDFPSVSREHAIAVLELAKTALLAKAAAA"
     gene            complement(2899..4074)
                     /locus_tag="VE170_17600"
     CDS             complement(2899..4074)
                     /locus_tag="VE170_17600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ17313.1"
                     /translation="MNGCLDGIKVLELGNFISGPYGAMLLADMGAEVIKVENPKGGDP
                     FRGWDLGGDEPNFWAYNRGKRSITLNLQTPEGKEIFLALAKTADVVLDNYRPGVMKKL
                     GIDYDTVSKTNPGVIYLSITGTGPSGPYVKRPAYDTVGQGLGGMLSLLMDAQNPRPIG
                     PNYADSLSGMFATIGVLGAIAARAKTGKGQQVDTTMAGSVLAFLIAAATDSLATGKIP
                     GPYTRPIQSQTYAFTGSDGAMFAIHLSSPQKFWEGLCKAAGHAELIDDPRFNTRPNRR
                     SNYDALANTFGEFFKEKPRAHWMARLEAEDVPHTPVYNMQEVFQDPQIQHMGLEITLE
                     RKDKPNIRTVAFPNQYSDSKNPHPLPPPELGEHNAEYLKPLGYTDQQIAEFKEKGII"
     gene            complement(4117..5292)
                     /locus_tag="VE170_17605"
     CDS             complement(4117..5292)
                     /locus_tag="VE170_17605"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYJ17314.1"
                     /translation="MSGCLDGIKVLELGNFISGPYGAMLLADMGAEVIKVENPKGGDP
                     FRGWDFGGDQPNFWAYNRGKKSVTLNLQTPEGKEVFYGLVKQSDVVMDNYRPGVTKKL
                     GIDFESLRKINPKIVCCSITGTGPSGPYVKRPAYDTVGQGMGGMMSVLMDPKNPKPVG
                     PAYADGLSGMFSALGVLGALVARASTGKGQQVDATMVGSILAFLNAPATETLAHGRIP
                     GPYTRPRQSQTYAFACADGAMLAIHLSSPQKFWEGLCNSVGHPEMIEDPRFKSRPDRR
                     ANYDELNKTFAVFFKEQPRAHWVEQLEANDVPHTPVYNLKEVFDDPQVKHMGMQIAIE
                     RKDKPTIKTVKFPLTHSGTPSPFPSPPPELGENNGEFLTALGYDQAKIAEFKEKGII"
     gene            5454..>5875
                     /locus_tag="VE170_17610"
     CDS             5454..>5875
                     /locus_tag="VE170_17610"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYJ17315.1"
                     /translation="MATPPPVTDRLARFVNDTDMSTMSAKTLADAKLHILDTLGVALA
                     GASTEVAEIALNYCRALGAASESSIWGTQTKAAVSSAAFANGLLAHALDYDDWDAFIH
                     VGHPSSMIVGAALPLAEHIGASGKDLLKAYVLGIEVIC"
BASE COUNT         1207 a         1804 c         1602 g         1262 t
ORIGIN      
        1 gcatgggcgg ccgcatcggc attcacttcg ccgctgcgac gccgagcgtg cgcgccttcg
       61 tcggatacta cccgtcggtg cgcgatgaag aaccgagcaa acttcggccg cgtcatccca
      121 acgacgccgt caaagatttc aaatgccctt cgcaaatctt cctcggcggc aatgaccatg
      181 tcgccagcat tccggtgcag caaaaattaa tggccagctt tcaagccaac ggccagccat
      241 tggactggca cttcttccag cgcgccggcc acggcttcgc catggccgac ggcgactgct
      301 acgatccgga gttggccgaa caggcatggg ggctgacggc gaatttcctg cggacagaat
      361 tagataatcg ctagccgagg attaacatta cgcaatcgta tagacgaagt tcgccgcatc
      421 actgcgttca tgccagccgg ctttgagata aaactgacgc cgataggatt cgcggttacg
      481 cagattaatc agggatagtc gttgacagct gcgtccccgg gattcgatct cgatgatttt
      541 cagcagttgg cggccaaccc cctgtccgcg catgtcatcg cgaacgaata attcagacac
      601 atatccttca ggccccgcca tgaaaagata cggcagccag tgcaccgagc cgtatccagt
      661 gatcatgcca tggggcgatt cggcgacata ggtcgagtga ctgtcgtccg cgagacattg
      721 tcgaagccgg ccttcgactc gcgcgatcga gtccttcaag tcccggctgc tgaacgcctc
      781 gaaccagcct atttcctgca gtaattttgc cagctgggcg gcgtcgtcga ttaccgcctt
      841 gcggatcgta atctccatcg aatcgctccg ttctgatgaa tcgctggaca tgatcatgcg
      901 actgctcagc taacacgccc ttcgattcac ggcaaatcga cattccagcg taacattaca
      961 ccacctgccc cgccgcccat cccgacgacc aagcccactg gaaattgaaa ccgccgagat
     1021 gaccggtgac atcgacgact tcgccgatga aatataggcc ggaaacgatc ttcgactcca
     1081 tcgtggtcga ggagagttcg ccggtgtcga cgccgccgag ggtgacctcc gcggtccgat
     1141 agccttcggt ttcgcccggc cgaaagcgcc aactttgcag ccgctcgcag atcccgcgca
     1201 tgtcccgcgc ggaatattgg cgtagcggcc ggctgcccag atcgatgtca cacaaccgct
     1261 gcgccaagcg tttcggtagg aattcaccga gcacggtctt taactccacg tccggtcgcg
     1321 ccgactgtcg gcctttcagc caagcgtcgg catcggtcag cggcataagg ttgatcatta
     1381 gatcatcgcc cggctgccaa taggacgaga tctgcaaaat cgccgggccg ctgacgccgc
     1441 gatgggtgaa caacatcgcg ttggcaaaac gctgccgtcg acattcgact tgcaccgcca
     1501 gactaacacc ggccagatct ttgtaacgca ccagatcgtc cgggctgaag accaagggcg
     1561 ccagaccggc acgcatcggc acgatcgcgt ggccgaatcg cttggcgagc tcgtagccat
     1621 acccagaggc gcccatcttc ggtaccgaca ggccgccaca agcaaccact aatgatgcag
     1681 ctccgctcga gcccagtttg ctcttgacga cgaacccggc agcgccacgc tcgacgctgt
     1741 ttacagtgca atcggtcgca atctgcactc ccgcctcgtg gcattcgtcg gtcagcagtt
     1801 tgaccacttg gcgcgccgat tgatcgcaga agagctggcc ccgttccttt tcgtgatacg
     1861 cgatgccatg gcgttccacc agcgcgataa aatcccattg agtgtagcgc gccagcgccg
     1921 acttgcagaa gtgcggattg gccgagagaa aattttccgg cgtgacatcg aggttggtga
     1981 agttgcagcg cccgccgccg gagatcaaaa tttttttgcc gacgcgatcg caatgttcga
     2041 tgaccagcac gcgccggccg cgcttgcccg cctcgatggc gcacatcaag cccgcggcgc
     2101 ctgcgccgat cactgctacg tcgtaatcga tcattcaccc gtcatacggc ggttctacta
     2161 ggcttcctga agcacgcgct gaaacgaagg gcgctcggca agtcgattta tgatggtaag
     2221 agagtatcat tttgaatcat caatgagtcc aatctcacga acctcgccag gtcgaatcta
     2281 gaaccgcccg tagctcaggc acaagcggaa gcagatccaa gaatctattt gtcttagcac
     2341 gcagaataat cacaccgaat aaacggctgc caaggttttg ctgatgtctc agactcttgt
     2401 cgacggtgat gaaagcatca aacccaccgc tgatggccgt cagtaacgga ccatcgtcga
     2461 gatctcccca acccaattgt ggcgcagtcc ggacttcgtg tgcggatatt tcgcgggcaa
     2521 gctttctagg cacgcagcta tcgagaagaa ctcgcacgct gctccaaggc aatcacgccg
     2581 cggcggcttt cgctagcaac gcggttttag caagctccaa aacggcaatg gcgtgctccc
     2641 gcgacacact gggaaagtct tccaggaaat cgttgagcga gtcgttcgct tcgagatatt
     2701 cgatgagggt ctgtaccgga actctcgtgc cagcaaacac tggtgcaccg ctcatgcgat
     2761 caggcgacag ggtgatcagt ggttcaggta atgacatctt cgactccgaa tccatgccgg
     2821 caatatacca caacccatcg cttgcggcga tccaagcaaa ccagagtcgc ttccccgcct
     2881 ctccgcgaga aagcgccctc aaattatccc cttctccttg aactcggcga tctgctggtc
     2941 cgtataaccc agcggcttca agtactcagc gttgtgttcg cccaactccg gcgggggtaa
     3001 ggggtgggga tttttgctgt cgctatactg gttcggaaac gccactgtgc ggatgttcgg
     3061 cttgtctttc cgttcgagag tgatctccaa acccatatgt tggatctgcg ggtcttggaa
     3121 aacttcctgc atgttgtaga ccggcgtgtg cggcacgtcc tcggcttcca gccgtgccat
     3181 ccagtgagca cgcggctttt ccttgaaaaa ctcgccgaag gtattggcga gagcgtcgta
     3241 gttgctgcgt cggttgggac gcgtgttgaa gcgcgggtcg tcgatcaatt ccgcatggcc
     3301 cgcggctttg cacaaccctt cccagaattt ctgcggcgac gaaaggtgaa tcgcgaacat
     3361 ggcgccatcg gaaccggtga atgcgtaggt ctgcgactga atcggccggg tgtacggacc
     3421 gggaatcttt ccagtggcga gcgaatcagt cgccgcggcg atcaagaacg ccaggacgga
     3481 gcccgccatc gtggtatcga cttgctggcc cttgccggtc tttgcccgcg cggcgatggc
     3541 gccgagcacg ccgatggtgg cgaacatgcc gctcaagctg tcggcatagt tgggaccgat
     3601 gggccgagga ttctgcgcgt ccatcagtaa gctcagcatg ccgccaaggc cttggccgac
     3661 ggtgtcgtac gccggccgct tcacgtaagg tccgctcggt ccggtgccgg tgatcgaaag
     3721 atagatcacc cccggattgg tcttgctcac ggtgtcgtaa tcgatgccaa gctttttcat
     3781 gaccccgggc cgataattgt cgagcacaac gtcggcagtc ttcgccaaag ctagaaagat
     3841 ttccttgccc tcgggagtct gcagattgag cgtgatactg cgcttgccgc gattgtacgc
     3901 ccagaagttc ggctcatcgc cgcccagatc ccaaccgcga aagggatcgc cgcccttggg
     3961 attttcaact ttgatcactt ctgcgcccat atcggcgagc aacatcgcgc cgtatggccc
     4021 ggaaatgaaa ttgcccagct ctaagacttt gatgccatcc aagcagccgt tcatgccatt
     4081 cttccttaaa aatgaattct cgtcgtcatt agtgaactag ataattccct tctccttgaa
     4141 ctcggcgatc ttcgcctggt cgtagccgag cgccgttaaa aactcgccgt tgttctcgcc
     4201 aagctccggc ggcggcgacg gaaacggcga cggcgtaccg ctatgggtca gcggaaactt
     4261 cacagttttg atcgtcggct tgtctttgcg ctcgatggcg atctgcatgc ccatgtgttt
     4321 gacctgggga tcgtcgaaga cttccttcag gttgtacacc ggcgtgtgcg gcacgtcgtt
     4381 ggcttcgagt tgctcgaccc aatgggcgcg cggctgttct ttgaaaaaca ccgcgaaggt
     4441 cttgttaagc tcgtcatagt tggcgcgccg gtccggccgc gacttgaagc gcgggtcttc
     4501 gatcatctcc ggatggccca ccgaattgca cagcccttcc caaaattttt gcggcgacga
     4561 cagatgaatc gccagcatgg cgccatcggc gcaggcgaag gcataggttt gcgactggcg
     4621 cggccgagtg tacggtccgg ggatacggcc atgcgccagc gtttcggtcg ccggcgcgtt
     4681 caggaatgcc aggatcgatc ccaccatggt cgcgtcgacc tgctggccct tgccggtgct
     4741 cgcgcgcgcc accagcgcac ccaaaacccc cagcgccgag aacatgccgc tcaaaccgtc
     4801 ggcgtaggcc ggccccaccg gcttgggatt ttttgggtcc atcaacacgc tcatcatacc
     4861 gcccatgccc tgacccacgg tgtcgtacgc cggccgtttg acatagggcc ccgatggacc
     4921 ggtgccggtg atcgagcagc aaactatttt cggattgatc ttgcgcagcg actcgaagtc
     4981 gatgccgagc ttcttggtca cgccgggacg atagttgtcc atcaccacgt cagactgctt
     5041 caccaaaccg tagaagactt ccttgccttc gggagtctgt aagtttagcg tgacgctctt
     5101 cttgccgcga ttataggccc agaagttcgg ttgatcgccg ccgaaatccc agccgcgaaa
     5161 cgggtcgccg cccttgggat tttcgacttt aatcacttcc gcgcccatgt ccgccagcaa
     5221 catcgcgccg tagggcccgg aaataaaatt gcccaactcc aaaaccttga taccgtctaa
     5281 acaaccgctc atgcttctgt ctccttaaat aatttcttac tatcggccga cgtctcttgg
     5341 cctcttagca ttcgcctttt actttttact ttttactttc cccttgcctg ttgcctattg
     5401 ccttcttaat ccgatattga actttcatat cgcccatttc gcaggagaac aagatggcaa
     5461 caccgcctcc ggtcaccgac cgcctcgctc gctttgtcaa tgacactgac atgtcgacca
     5521 tgtcggcgaa aactttggcc gatgccaagc tgcatatcct ggataccctc ggcgtcgcgc
     5581 tagccggagc atccacagag gtagcggaga tcgcgctcaa ttattgccgc gcactcggcg
     5641 cggccagcga gtcgtccatc tggggcaccc agacaaaagc cgccgtcagc agcgccgctt
     5701 tcgccaatgg cctgctggcc catgccctcg attacgacga ctgggatgcc tttattcatg
     5761 tcggtcatcc gtcgagtatg attgtcggcg cagcgctgcc tctggcggag cacatcggcg
     5821 cttccggcaa agacttgctc aaggcctacg tgctcggcat cgaagtgatt tgtaa
//
LOCUS       DASQKS010000679         6561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4556835, whole genome shotgun sequence.
ACCESSION   DASQKS010000679 DASQKS010000000
VERSION     DASQKS010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4556835"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VE170_17615"
     CDS             <1..740
                     /locus_tag="VE170_17615"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17316.1"
                     /translation="AAPAPLRANLKGEADVIFLTGGINQQFLVGRPGINSREELSGKK
                     IGTVNDGGIGDALVKFVVAKLDGAGIKGLQEVTIGHGAEAEMSALLSGTVDVLVGTPP
                     ECIDAIRHGCKYLIDFAEYGLNYALGGIAARRDYIEKNPAITRQFVKAYVEGMHRYRT
                     DREFSVDVQADYSGIADRTIAEETYDLTQPGMPRAPYPVVASLQTLLDFMAKDLPEAR
                     GANAGKFVDESFIRELEDNGFIASLGR"
     gene            747..1751
                     /locus_tag="VE170_17620"
     CDS             747..1751
                     /locus_tag="VE170_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17317.1"
                     /translation="MAKLNISCVSLLWLAVSAANLSAATNDALIGAAKREGELVYYAS
                     MNLAEANAIISEFEKRYPFIKVRLHRTGSEKLLTRVLTEYRAKKTFADIIQTVEFSMH
                     IFNRSGVLARYLPLSNGLYSKDFKEEGFWTTVYYNAYVTAYNTRLVPPGQLARSYDDL
                     LDSKWKGKLLMEGTKADWFAGMLQILGPERGLKYMRELAKQQPSPREGHELLAQLVVA
                     GEGAYDINIPASSVERMKERGAPIDWTALGPVPAIMVGAGIATGAVHPNAAKLFLEFI
                     LSREGQKLMGTPGRHIARSDLANEQAAILKDLKIVPVNPALAEKLDEYAKQMRGIFGR
                     "
     gene            1803..2567
                     /locus_tag="VE170_17625"
     CDS             1803..2567
                     /locus_tag="VE170_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYJ17318.1"
                     /translation="MFLNHSAERHSHELEVHIAKLLKSYASPDSTFELAYPEDLGSSA
                     VLKLLEERKALSGLHHILETPALVKKAIEAERQGFDAVIQSNTFDPGVEASRLAVRIP
                     VIGLLRASLHFAATICDRFGLIVPLETHMPHTLRMVQTYGMTPFVSGIRTVGLYDTGD
                     LSGYHDVVIERTLAVGKELVAQGAQAIIPLGGKIYPYVVTPEELEPHLGVPVINTKAV
                     GVSYAELMARNSIKHSIKAYPCPTGLDPDAVSARSK"
     gene            2648..3742
                     /locus_tag="VE170_17630"
     CDS             2648..3742
                     /locus_tag="VE170_17630"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17319.1"
                     /translation="MIIDGDGHFFETEEIFDKYMEPALRNFRPRLLGDEQGHNFWVVD
                     GHTSYKRPTIKGAGAPGTAAPPGKALQSARRASPGSQTLNNLKERLDDLDREKIDLQF
                     IYPSFLLHANAWPDGILGNGICRAYNTWLNQACNQAPDRLQAVGVVSLLDPQGAAKEI
                     TRLSAMGVKAVMINGTAGARRLDHPDHDVFFAECDRVKMPIAVHFSLQFPFVDTLFEH
                     HFPNRVLAGIFPIMAGLTSVLCSGLLDRYKNLKFAFLEGGISWVPAHVERMDDHFDNP
                     RYGAKDLISHPPSEYLKSGRIFFGCEGNEATLPKIVGEVGDDLFMFSSDFPHADRTEG
                     TATILQERGDIAGPVRKKLLEDNARQFYGI"
     gene            3963..4145
                     /locus_tag="VE170_17635"
     CDS             3963..4145
                     /locus_tag="VE170_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17320.1"
                     /translation="MELPKCQKCNNGVLIPLSDYGQEGASVMYKAWACTNPDCGFSLR
                     VDKGEVTYGKKIEHKH"
     gene            complement(4207..4863)
                     /locus_tag="VE170_17640"
     CDS             complement(4207..4863)
                     /locus_tag="VE170_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17321.1"
                     /translation="MEALQPDEFLRQLIATREAFWEKMNAERKSYGAEKPLTPAIVLR
                     RLQFGVVMERKAAEVTAPWVSKIPDLDLQQPMSEYVVNELRHTSILRQRICDLKGDPD
                     SLWNSPLAELKQLWDFHAGLGSLCELIASVQFGHEEFFPRTSKSFIERVGAIDPITAA
                     IYRDVLLAEEEGHEWIAPEILKRYATDAESQQQCLTALKTGCELFGRAIQSFNQSMPA
                     "
     gene            4992..5735
                     /locus_tag="VE170_17645"
     CDS             4992..5735
                     /locus_tag="VE170_17645"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17322.1"
                     /translation="MESSQAKIYVDDLYDQVHRHWEKRIHNGEFMTRLRDGTLPLPCL
                     RRFFKDWGLFSIEVVALNAVSYYVHLPFFVENFDLLAPLCDKVAEELVSPKPPGHILI
                     LLETANALGLSKQEMFEQPASAPGRAISDYCRRVFQDGSIIELWGAHVYEETLGHWSQ
                     QWSKALVGHYGMSKSQAIYFNAHAEADLVQHENHMGHGPLNRMILQRILEQGKVAKKL
                     GYDPKYCAFTMVDLQALMEQHALDNPYPA"
     gene            5848..>6561
                     /locus_tag="VE170_17650"
     CDS             5848..>6561
                     /locus_tag="VE170_17650"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HYJ17323.1"
                     /translation="MTIAFAAKAPAQLPGKWITRAPMPSARTEVAAVELGGKIYVIGG
                     YDKGSHLVEEYDPATDSWRGRASLPKPLHHVGAAALNGKIYVIGGYISGVGPVDTVYE
                     YAPAKDQWTPKEAMTTARGALTVGVVSGKIYAVGGIGKNGRDTPANESYDPAQDLWRK
                     HRGMITARDHHAVGVEDGKLYAIAGRINGNHDRSIGANEEYDPVSNRWRVRSPIPTVR
                     SGVVAATLGGRIFVFGGEHG"
BASE COUNT         1480 a         1939 c         1796 g         1346 t
ORIGIN      
        1 tggcggcgcc ggcgccgctg cgagctaatc tcaagggcga agccgacgtg attttcctca
       61 ccggcggcat caatcaacaa tttctagtcg gccggcccgg catcaatagt agagaagaac
      121 tgtccggcaa aaaaatcggc acggtcaatg acggcggcat cggcgatgcg ctggtaaagt
      181 tcgtcgtcgc caaactcgac ggcgccggca tcaaaggcct gcaagaagtg accatcggtc
      241 atggcgccga ggcagaaatg tccgcgttgc tcagtggcac ggtcgatgtc ctcgtcggta
      301 cgccgccgga atgcatcgac gccatccgcc atggctgcaa gtatctgatc gacttcgcag
      361 aatacgggct taactatgcg ctgggcggca tcgcggcgcg gcgcgattat atcgagaaga
      421 atcccgcgat cacccgccag ttcgtcaaag cttatgtcga gggcatgcat cgttaccgca
      481 ccgaccggga attcagcgtc gatgtccagg ccgactatag cggcatcgcc gaccgcacca
      541 tcgccgaaga gacctatgat ctcactcaac ccggcatgcc gcgcgctccc tacccggtag
      601 tcgcctcact gcaaaccttg ctcgatttca tggccaagga tttacccgaa gccagaggcg
      661 ccaacgccgg gaagttcgtc gacgagagtt ttatccgcga actggaagac aatggcttca
      721 tcgcttcatt agggaggtag ttgcaaatgg ccaagctcaa cataagctgc gttagcttgt
      781 tgtggctggc ggtctcggca gcaaacctca gcgccgcgac taacgatgcc ttgatcggcg
      841 ccgctaagcg cgagggcgag ctggtctact acgcgtcgat gaatcttgcc gaagccaacg
      901 caatcatcag cgaattcgag aagcgctacc cattcataaa agtaagactg caccgcaccg
      961 gcagcgaaaa gctattgacg cgggtcttga ccgagtaccg tgccaaaaaa accttcgccg
     1021 atatcattca gaccgtcgag ttcagcatgc atattttcaa tcgcagcggc gtgctggcgc
     1081 gttacctgcc gctatcgaac gggttgtatt ccaaagactt caaggaagag ggattctgga
     1141 ccacggtcta ttacaacgcc tacgtgaccg cctacaatac ccgcctggtg ccgccggggc
     1201 agctggcgcg atcctatgac gatctgctcg atagtaaatg gaagggcaag ctcttgatgg
     1261 aaggcaccaa ggccgattgg ttcgccggca tgttgcaaat tcttggaccg gagcgcgggc
     1321 tcaaatacat gcgcgaactc gccaaacaac agccaagccc gcgcgaaggc catgagctct
     1381 tggcgcagtt ggtcgtcgcc ggcgaaggcg cctacgatat caatatccct gcgtcgtcgg
     1441 tggagcgcat gaaagaacgc ggcgcgccca tcgactggac cgcgctgggg ccggtgccgg
     1501 cgatcatggt cggcgccggc atcgccaccg gagcagtcca tcccaatgcc gccaagttat
     1561 ttttagaatt catcctgtcg cgcgaggggc agaagctcat gggaaccccc ggccgccata
     1621 tcgcccgcag cgatcttgcc aacgaacagg cagcgatact taaagatctc aaaatcgtcc
     1681 cggtcaatcc ggcgctggcg gaaaagctcg acgaatatgc caagcagatg cgcgggattt
     1741 ttggccggta gctgaataat caagccgatt tataaacagc gaaggagaaa atcatgcgtt
     1801 tcatgttttt aaaccactcc gcggagcgtc actcccacga gctcgaagtc cacatcgcca
     1861 agctgctcaa aagctacgcc tcgccggaca gcacttttga gctggcttat cccgaagatc
     1921 tgggcagcag cgccgtattg aaactgctcg aagagcgcaa agcgctctcc ggcctgcatc
     1981 atattttgga aaccccggcg ctggtgaaga aagccatcga agccgagcgt cagggtttcg
     2041 acgcagtgat ccagagcaac accttcgacc ccggtgttga agcgagccgt ctcgctgtgc
     2101 gcatcccggt gatcggcctg ttgcgcgcat cgctgcactt cgccgccact atttgcgacc
     2161 gcttcggtct catcgtgccg ctggaaaccc atatgccgca caccctgcgc atggtgcaga
     2221 cctatggcat gacgccgttc gtcagcggca tccgcaccgt cggtctctac gataccggcg
     2281 atctctccgg ttatcatgac gtcgtcatcg aacgcacttt ggcggtcggc aaagaactgg
     2341 tggcccaagg cgcccaagcg attattcctc tgggtggcaa gatttatccc tacgtggtca
     2401 cgccggaaga attggaaccc cacctcggcg ttcccgtgat caacaccaaa gccgttggcg
     2461 tgagctacgc cgaactcatg gcgcgcaact ccattaagca cagcatcaaa gcctatccct
     2521 gtcccaccgg cctcgacccc gatgccgtat ccgcgcgttc gaagtagccg gtcgaggccg
     2581 agttgcattg gcttaaagca gtcattatga gattagtttt aaattcacta gaggagatcc
     2641 gtaggcaatg attatcgatg gcgacggaca tttttttgaa accgaagaga tcttcgacaa
     2701 gtatatggag ccggctctgc gcaatttccg gccgcgcctg ttgggcgatg aacaaggcca
     2761 caacttttgg gtggtcgacg gccatacgtc ttacaaacgg ccgaccatca aaggcgccgg
     2821 cgcgccggga accgccgccc ctccgggaaa agccctgcag tcggcgcgcc gagcctcgcc
     2881 gggaagccag acactgaaca atttaaagga gcgcttggac gatctcgatc gcgaaaaaat
     2941 cgacctgcag ttcatttatc cgagcttttt gcttcatgcc aacgcctggc ccgacggcat
     3001 tttaggtaac ggcatctgcc gcgcgtataa cacttggctc aaccaagcgt gcaaccaagc
     3061 ccccgaccgt ttgcaagcgg tcggcgtcgt gtccttgctc gatccgcagg gcgcggccaa
     3121 agaaatcacc cggctgagcg ccatgggagt gaaagcggtg atgatcaacg gcaccgccgg
     3181 cgcccggcgt ttggaccatc ccgaccatga cgtcttcttc gccgagtgcg accgtgtcaa
     3241 gatgcccatt gcggtgcatt tcagtttaca attccccttc gtcgacacgc ttttcgaaca
     3301 ccattttcca aaccgcgtac ttgccggtat ttttccgatc atggccggat tgaccagtgt
     3361 gttgtgctcg ggccttttgg accgctacaa gaatttaaaa ttcgcctttc tcgaaggcgg
     3421 catcagctgg gtgccggccc atgtcgagcg catggacgat cactttgaca acccgcgcta
     3481 cggcgccaaa gatctaatca gccatccacc cagcgagtat ctcaaaagcg gccggatttt
     3541 cttcggctgc gagggcaacg aagcgacatt gccaaaaatc gtcggtgaag ttggcgacga
     3601 tctattcatg ttctcttctg acttccccca tgccgaccgc accgaaggca cggcgaccat
     3661 tctccaagaa cgcggcgata ttgccggacc cgtgcgtaag aaactcctgg aagacaacgc
     3721 gcggcaattc tacggcatct aggccagaat cgaaaagcgc gaccatcaac gcgggggacg
     3781 aacagagcaa agggatagtc ggcgctgtcg actatccctt tttgcgtcta gggcgattaa
     3841 tcgcgataaa tcgctttacc cccgctccct tgtcatgctt gatttatcct ctgccaatac
     3901 gataaaattt tgctgcgtga atgagctgaa accaggctga ggtgttgaaa gaaggagtgg
     3961 agatggaact gcccaaatgt cagaaatgca ataacggggt attgataccg ctgtcggact
     4021 acgggcaaga gggcgcctca gtaatgtaca aagcctgggc ttgtactaac cctgactgcg
     4081 gtttttcctt gcgcgtcgac aaaggcgaag tcacctacgg taaaaagatc gagcacaaac
     4141 actaaattcg tttcgcctca tgagcgtgtt cgtggcccgc agatgatccc gccgaacgac
     4201 acgatctcaa gccggcatgc tctggttgaa gctctgaatc gcccggccaa acagctcaca
     4261 accggttttc aatgcggtca gacattgctg ctgcgactcg gcatcggtgg cgtaacgttt
     4321 gagaatctcc ggtgcgatcc attcatggcc ttcttcctcg gcgagcaata catcgcggta
     4381 gatcgccgcg gtgatcggat cgatggcacc aacccgctcg atgaaggatt tcgaggtccg
     4441 cggaaaaaac tcttcgtgac cgaactgcac gctggcgatc agctcgcata acgagcccaa
     4501 gccggcatga aaatcccaca gctgttttaa ttccgccaat ggactgttcc ataagctatc
     4561 cgggtcaccc ttgaggtcgc aaatgcgctg gcgcaggatc gaggtatgtc ttaattcgtt
     4621 gacgacatac tcggacatcg gctgttgcag gtcgagatcg gggatcttgc tcacccaagg
     4681 cgcggtgact tcggcagctt tccgttccat gactacgccg aactgcaagc ggcgcagcac
     4741 gatcgccggg gtcaacggct tctcggcgcc gtagctcttg cgctcggcat tcatcttttc
     4801 ccagaaggcc tcacgcgtgg cgatcaactg tcgcaaaaat tcgtctggct gcagcgcttc
     4861 catgattcct ccgccgcttg ctgtttcagt gattgactcc gccaatcatt taggttttaa
     4921 caatacaaga atttgagccc ttgtaaaagc tggtcaaccc ccccgaagca ctcaaaaaat
     4981 ctaagaattt aatggaatct agccaagcaa aaatctatgt cgacgatctt tacgatcaag
     5041 tgcatcgcca ttgggaaaag cgcattcaca acggcgagtt tatgacccgc ctgcgtgacg
     5101 gcacgctgcc gttgccctgc ctacggcgct tcttcaaaga ctgggggctg ttttccatcg
     5161 aggtggtcgc cctcaacgcg gtgtcgtact acgttcacct gcctttcttc gtggaaaatt
     5221 tcgatttgtt ggcgccgctt tgcgacaagg tcgccgaaga actggtttca cccaaaccgc
     5281 ccggccatat cctgatcctg ctggaaaccg ccaacgcatt gggactaagc aaacaagaaa
     5341 tgttcgagca accggcatcg gcgccgggtc gggcgatcag cgattactgt cggcgggtgt
     5401 ttcaagacgg ctcgatcatc gagctctggg gcgcccatgt ttacgaagaa accctcggcc
     5461 actggtcgca gcaatggagc aaggcgctgg tcggccacta cggcatgagc aaaagtcagg
     5521 cgatttactt caacgctcac gccgaagccg acttggttca gcatgaaaat cacatgggcc
     5581 atgggccgtt gaatcgaatg atactgcagc gcattcttga gcagggcaag gtcgccaaaa
     5641 aattaggtta cgatcccaaa tactgcgcct tcaccatggt cgacctgcaa gcgctcatgg
     5701 agcagcacgc cttggataat ccctacccgg cgtaaacgtc gatttctggc gcagcggtaa
     5761 ttttcgctct gcgctgttgg attccatata taaactgatc ccgatgcctt gatgcgcgcg
     5821 cgaatttttt tctcactctg tctcctcgtg accattgcct tcgcggccaa agcgccggcc
     5881 cagttgcccg gcaaatggat cacccgtgcg ccgatgccct cggcgcgcac cgaggtcgcg
     5941 gcggtggaac tcggcggcaa gatttacgtc attgggggtt acgataaagg cagccatctg
     6001 gttgaggaat acgacccggc aaccgatagt tggcgcggcc gcgcctccct gccaaagcca
     6061 ctgcatcacg tcggcgcggc ggcgctcaac ggcaagattt atgtcatcgg cggctatatt
     6121 tccggcgtcg gtccggtcga taccgtctat gaatacgctc cggccaaaga tcagtggacg
     6181 ccgaaggaag cgatgacaac cgcacggggc gctctcaccg tcggcgtcgt ctctggaaaa
     6241 atctatgccg tcggcgggat tggcaagaat ggccgcgaca cgccggccaa cgaatcctac
     6301 gatccagctc aggacctttg gcgcaagcac cgaggcatga tcacagcgcg ggatcaccat
     6361 gccgtcggcg tcgaagacgg caaactctac gccatcgccg gccgcatcaa cggcaatcac
     6421 gatcgcagca tcggtgctaa cgaggaatac gatcccgtga gcaaccgctg gcgcgtgcga
     6481 tctccgatcc cgaccgtgcg cagtggcgtc gtcgcggcca ctctcggtgg gcgcatattt
     6541 gtcttcggcg gcgaacatgg c
//
LOCUS       DASQKS010000680         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5006895, whole genome shotgun sequence.
ACCESSION   DASQKS010000680 DASQKS010000000
VERSION     DASQKS010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5006895"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3445
                     /locus_tag="VE170_17655"
     CDS             <1..3445
                     /locus_tag="VE170_17655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYJ17324.1"
                     /translation="GSLKFESTHRTKDGRRLRTELIVNYIVYEDEEYNCAIMRDITDR
                     KQIEDALRRRDRQLVEAERIGQMGSWELDLLTNRLEWSDESFRIFESDPTAFGASYQA
                     FLNLVHPDDRLSVDQAYSGSVRDHAPYQITHRLLLADGRVKWVDQRGETFCDDDGRAL
                     RSMGSVLDITARKQAELAVRASEQSIRRLYEITNAIELSFDERVSALLAFGCARFNLP
                     YGVLTFLQGDELEILSAHPPDGSLAPGTRLPLRASYCSYVVEADEPVCNEHIAVSEWR
                     NHPGYLTLGVESFMGSKIDINGLLYGALCFYGPMPYRGHFSDSDKDFLRLMAVWIGGE
                     VDRYQAHQQLQKSHDQIRQIIDTDPNFIFARDGEGRYTLVNKALADAYGTSVDNLVGK
                     TDADFNSNAAEVETYREMDLEVMDTLQEMFIAEQTITDSSGRKRWLQTVKRPILDASG
                     RASQVLGVSADITARKVAEKALRQSEQHLVDAQAIAHLGSWEWDFQTGRSTWSDENYR
                     LLGYEPGSVAPSYDSWINRIHPDDRSRVQEHLSSAFNGTTHCDTEFRVLHPNNEIRFV
                     HWRGELFRDAGGNELRMAGTVLDITERKRAEQALRQAHDELERRVEERTAELQQAVAT
                     LKKTEVLLHQAVDVANLGIFERAHDSDAVHYSSTLRKTLGLPEDREGSLGEFLAKIHP
                     DDQATVIAARRQSREGAGDGQFSLECRVIRGDGSLGWVMVRSQVFFEATGDGRKPART
                     VGAVLDITERKRAEEALRKLTENLDRRVVERTRELAESQSRLRSLVAEITKTEERERR
                     RLAVELHDYLAQMLTVSRMNIARAEKLAASDPLKQRLAEALESVDESIAYTRSLMAQL
                     SPRMLYELGLPAALTWLAAQQQERYGLKIEIAGTADGFALDEEVSVLAYQCVRELLWN
                     IVKHAQADHALVSYKIEDCELIVEVTDDGQGFDPESLHASATGAEKFGLFSTRERLES
                     SGGKLEVSSQPGRGTRVRFTLPAPPSEITPAVAHGVVPNPATLGREKQLRIALVDDHM
                     VVRRGMRQMLEEYTDFTVVGEAIDGLAGVELAREFRPDVVVMDVNMPRMNGIDATKLI
                     LQEMPATIVVGLSFENGVEMAQKMKAAGAFTCITKERAADDIHQAIIDAVRERRGVVT
                     D"
BASE COUNT          771 a          947 c         1101 g          686 t
ORIGIN      
        1 cgggtcgtta aaattcgaat ccacgcatcg caccaaggac ggcaggcgcc tgagaaccga
       61 actgatcgtc aactacatcg tatatgaaga cgaagaatat aactgcgcca tcatgcgcga
      121 cattaccgat cgtaaacaga tcgaggacgc gctgcggcga cgggatcgtc agctggtcga
      181 ggcggagcgc atcgggcaga tgggcagttg ggaattggat ctgctgacaa atcgtctcga
      241 atggtctgac gaaagctttc gcatctttga aagcgatccg accgcgttcg gcgcctccta
      301 tcaagcattt ctcaatctcg tccaccccga tgaccggttg tcggtcgatc aagcgtacag
      361 cggttcagtg cgcgaccacg cgccgtacca gattacccac cgcctgttgc tggccgacgg
      421 ccgcgtcaag tgggtggacc aacgcggtga gaccttctgt gacgacgacg gacgggctct
      481 gcgatccatg ggaagcgtgc tcgacatcac cgcccgcaag caggcggagt tggcggttcg
      541 tgccagcgaa cagagcatcc gccggttgta cgagataacc aatgccattg agctttcctt
      601 cgatgagcgc gtgagcgcgc tgctggcgtt cggctgtgca cggtttaatc tgccttatgg
      661 ggtgttgact tttcttcagg gtgacgagct ggaaatactc agcgctcatc cgccggacgg
      721 gagccttgcg ccaggaacga gattgccgtt acgcgcctcc tattgcagct atgttgtcga
      781 ggccgatgaa ccggtttgta atgagcatat cgccgtctcg gaatggcgca atcatcctgg
      841 ctacctgacc ctcggtgtgg aatcgtttat gggcagcaaa attgacataa acggcctgct
      901 ctacggcgcg ctgtgttttt acggcccaat gccgtatcga ggacatttta gcgattccga
      961 taaggatttt ctgcggctca tggcggtatg gatcggcggc gaggttgacc gctaccaggc
     1021 tcatcagcaa ctgcaaaagt cccacgacca gatacgccaa attatcgata ccgatccgaa
     1081 ctttattttt gcccgagatg gcgagggccg ttataccctg gtgaacaaag ctttggcaga
     1141 tgcctatggc accagcgtgg acaaccttgt cggcaaaacc gacgcggact tcaactccaa
     1201 cgccgccgag gtggagactt accgcgaaat ggatctggaa gtgatggaca cgcttcaaga
     1261 gatgttcata gcagaacaaa ccatcaccga ctcgagcggc cggaaacgtt ggctgcaaac
     1321 cgtcaaacga ccaatactgg atgcgagcgg ccgtgcgagc caggtgctcg gcgtgtcggc
     1381 cgatatcact gcgcgcaaag tggcagagaa agccttgcgc cagagcgagc aacatctcgt
     1441 ggatgcccag gcgatcgctc accttggcag ctgggaatgg gatttccaaa ccggccgaag
     1501 cacgtggtcg gacgaaaact atcgtcttct cggttacgag ccgggctcgg ttgcgccgag
     1561 ttacgatagc tggatcaata ggattcatcc cgatgatcgt tcgcgagtcc aggagcattt
     1621 gagctcggca ttcaatggaa cgacccattg cgacaccgag tttcgggtcc tccatccgaa
     1681 taacgagatt cgcttcgttc attggcgcgg cgaattgttt cgcgatgccg gcggcaatga
     1741 gctgcgcatg gcgggtacgg tgctcgatat caccgagcga aaaagagcgg agcaagcgct
     1801 gcgccaggcc catgatgaac ttgagcgccg cgttgaggag cgaaccgccg agctgcagca
     1861 ggcggttgct acgctgaaga aaaccgaggt gttgctgcat caggctgtcg atgtggcgaa
     1921 cctcggcata ttcgagcgcg ctcatgattc cgatgcagtg cattattcgt ccacgttaag
     1981 aaaaacgttg gggttgcccg aagatcgcga ggggagcctg ggcgaatttc tggcaaaaat
     2041 acaccctgac gatcaggcga cggtcatcgc cgcgcggcga cagtcgcgtg agggggcggg
     2101 tgacggtcaa ttttccctgg agtgccgcgt cattcggggc gacggtagcc tcggctgggt
     2161 tatggttaga tctcaagttt tctttgaagc cacgggcgac ggccgcaagc cggcgcgcac
     2221 tgtcggcgcc gtgctcgata tcaccgagcg caaacgcgcg gaagaagcgt tgcgcaaact
     2281 gaccgaaaat ctcgatcgcc gggtggtcga gcggacccga gaattggccg agtcgcaatc
     2341 gcgcttacgc tcgctggtgg cggagatcac caagacggag gagcgcgaac ggcgccggct
     2401 ggcggtggag ctgcacgatt atctcgcgca gatgctgacc gtcagtagaa tgaatatcgc
     2461 cagggcggaa aagctcgcgg cgagcgatcc gttgaaacaa cgcttggcgg aggcactgga
     2521 gtcggtggac gagtcgatcg cttatacacg ttccctgatg gcccaactga gtccgcgcat
     2581 gctttacgag cttggactgc ccgcggcgct tacctggctc gccgcccaac agcaggaaag
     2641 gtatggactc aagatagaga ttgccgggac ggccgacgga tttgcgctcg acgaagaagt
     2701 ttccgtgttg gcttatcaat gcgtgcgcga gctgctgtgg aatatcgtca agcatgcgca
     2761 ggccgatcat gcattggtat cctataagat cgaggattgt gagttgatcg tcgaagtcac
     2821 cgacgatggc cagggatttg acccagagag tttgcatgca agcgctaccg gcgccgagaa
     2881 gttcggcctg ttcagcaccc gcgaacgact ggagtcgtct ggcggcaagc tggaagtgtc
     2941 gtcgcagccg gggcgcggca cgagggtacg atttaccttg ccggcgccgc ccagcgaaat
     3001 aacaccggcg gttgcccacg gcgtcgtgcc aaaccccgcg acgctggggc gcgaaaagca
     3061 gttgcgtatt gcgctggtcg acgaccatat ggtggtgcgc cggggcatgc gccagatgct
     3121 cgaggagtac accgacttca cagtggtcgg cgaagctata gatggcttgg caggcgtcga
     3181 gttggcgcgc gagtttagac ccgatgtagt cgtgatggat gtcaatatgc cgcgcatgaa
     3241 cggcatcgac gcgaccaagt taattctcca agaaatgccc gcgaccatcg tggttggatt
     3301 gtccttcgaa aacggtgtgg agatggcgca aaaaatgaaa gctgccggcg ccttcacctg
     3361 cattaccaaa gaacgagcgg cggacgatat tcatcaagcc atcatagacg ccgtgcggga
     3421 gcggcgcggg gtggtaacgg attaagccgg cgatgccccg ggcgggatgc gaaagagttc
     3481 ttaccgcgaa caatgcggcg cggcg
//
LOCUS       DASQKS010000681         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_814275, whole genome shotgun sequence.
ACCESSION   DASQKS010000681 DASQKS010000000
VERSION     DASQKS010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814275"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..637
                     /locus_tag="VE170_17660"
     CDS             83..637
                     /locus_tag="VE170_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ17325.1"
                     /translation="MNQDDKGRQFQQAFLPHLSAAHNLARWLVQNDHDADDIVQDAYL
                     RALRFFDGFRGGDSRAWLLTIVRNRCYTWLRNRTLADNVAAFDERQHSPSVNDATDTG
                     GIKFTDPAVLLAQLDDAAQLNMAIASLPEEFREAIVLRELEELSYKQIATITGVPMGT
                     VMSRIARGRKMLLQRLQCLEQKKP"
     gene            634..1431
                     /locus_tag="VE170_17665"
     CDS             634..1431
                     /locus_tag="VE170_17665"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYJ17326.1"
                     /translation="MTESCQRSRELASAYLDNELSATESLAVERHLHECADCSREASA
                     LAALSGKIRSQARYHIASPALADRVRTASAPATLSQPVTKFSKPGFFSRWVPMTGAAA
                     MAILIAGVALWQFAGRNVETRLSQEILSSHIRSTLSQQPFDIASADRHTVKPWFRGKL
                     NFAPPVVDLAAQGFVLQGGRLDFVDGRTVPVIVYGRRKHVVNLYVWPNPGHADQPPKP
                     SSVNGYNLIGWQSDGMRLSAISDLAAGELAEFARQFIMKSASEAPNS"
     gene            complement(1441..2739)
                     /locus_tag="VE170_17670"
     CDS             complement(1441..2739)
                     /locus_tag="VE170_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HYJ17327.1"
                     /translation="MVCVGIAACLSPGVDQAFAQGRIVGISQFPPISIKDFQNARFAG
                     SVANNRKVLLGSVGSDLWRGAEDAAGEFWMVTDRGPNGQIRVDGANRRTFWVPEFNPT
                     IVRVKLEGGAIKIVETVPIVGRSGKPITGLPNARTIDETPYDYTAQKEFGFNVNGLDT
                     EGLIRTKSGEFWLADEYSPSLIHADKTGKVIKRYVPAGVKLPGADYPVQESLPAIFGK
                     RKINRGFEGLAVSADEKTLYAVLQSPLANPDRKIGDASRNTRVVVFDRDQEQVTAEYV
                     YRFEVAKDFDPRPKMSPDEMKLSAVVYLNPTTLLVLERTDWSAKLYSVDLTQATNILK
                     SKWDEASTTPSLEALDDPASAAIRVLPKTLVLDLNQFKEMPEKIEGVTVIDRNTIAVA
                     NDNDFDSEESKYDEQGNNIGKGKVSQILVIALDKPLPLAK"
     gene            complement(2939..3898)
                     /locus_tag="VE170_17675"
     CDS             complement(2939..3898)
                     /locus_tag="VE170_17675"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17328.1"
                     /translation="MKNQLNVFALIVAVLLSVPALAADKVIISYSSRSYAFLPAQIAV
                     VKGFFKDENLEPLLIQMRSQVTVPALLSGEVHYTLSFGNIIGSAMAGLPFKILGVLTD
                     KPLHSIVARPEIKTIGDMRGKRMGSQRIGGSDQLAAEAIFQAKGIDVKDVQFVTLGGD
                     EPVRTEMLRKGLVDAICTVPPGPVRLAREGYNVLGGPKDLKIGSPISAVAVTDVRLKS
                     HREETKRVLRAMLRGLRVMHERRDDTLAIMGKWLSQSADVARDSYDSILPSFSLDGST
                     VDKTYEFAIESRKATVRTDKPIPLSQVRDLSLLREVQKELRMQ"
     gene            complement(3895..4599)
                     /gene="bioD"
                     /locus_tag="VE170_17680"
     CDS             complement(3895..4599)
                     /gene="bioD"
                     /locus_tag="VE170_17680"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HYJ17329.1"
                     /translation="MGQAIFITGTDTGVGKTFFTCGLARLLRSYGYRVGVMKPAETGC
                     VMRNGMLYPEDAWQLKKASECAVPIEKVCPYPLPEPLAPSIAAERAGTRIDIDHLLAV
                     FDEIKAAHDITLVEGAGGLMVPLLPSYTFADFVRVAKLPVIVVAANRLGAINHLLLTL
                     EHASCKELRLLGYVLNRLSNEDSLAAETNREVLLGLTGVACLGELPFQGSGAALTDSF
                     EAEIDFRTIDLWIGKT"
BASE COUNT          963 a         1349 c         1308 g         1015 t
ORIGIN      
        1 gcgataaaag tatttcgcat cactatggga ataaacagct tggcgccggg tcttacgtta
       61 atgggtgtct gtttgaggac atgtgaatca ggacgacaaa ggcaggcagt ttcagcaggc
      121 gtttttgccg catctctcgg ccgcccataa tttggcacgc tggctggtgc agaacgatca
      181 cgatgccgac gatatcgtgc aggacgctta tctgcgcgct ttacgctttt tcgatggctt
      241 tcgcggcggc gacagccgcg cttggctgct gaccatcgtc agaaatcgtt gttacacctg
      301 gttgcgcaac aggaccttgg ccgacaacgt cgcggcattc gacgaacgac agcattcacc
      361 gtcggtgaac gacgcgactg acacgggggg aataaaattc accgatcccg cggtcttatt
      421 ggcgcaactt gacgacgccg cgcagctcaa catggcgatt gccagcttgc cggaagaatt
      481 tcgcgaagcg atcgtcctac gtgagttaga ggagttgtct tacaaacaga tcgcgacgat
      541 caccggcgtg cccatgggca cggtgatgtc gcgcatcgcg cgcgggcgca agatgctact
      601 gcaacgattg cagtgcttag agcagaaaaa accatgaccg aatcttgcca gcggtcgagg
      661 gaactcgcct cggcatatct cgacaacgag ctcagcgcca ccgaaagctt ggctgtggaa
      721 cgccatctgc acgaatgtgc cgactgctca cgggaggcgt cggccctggc cgcgctttcc
      781 gggaaaatca gaagccaagc ccgctatcac atcgcgtcgc cggcgctcgc cgatcgagta
      841 cgcacggcct ccgcacccgc aacgttgagc caaccggtaa cgaaattctc gaaaccaggc
      901 ttcttctctc gatgggtgcc aatgaccggc gctgccgcaa tggcgattct aattgcggga
      961 gttgcgcttt ggcagttcgc cgggcgcaat gttgagaccc ggttatccca ggaaatttta
     1021 tcgagccaca tccgctcgac tttgagtcaa caacccttcg atatcgcatc tgcggatcgc
     1081 catacggtca aaccttggtt tcgcggtaaa ttgaattttg ctccgcccgt cgtcgatctt
     1141 gccgcgcagg ggtttgtctt gcagggcgga cgcttggact ttgtcgatgg ccgtactgtc
     1201 ccggttattg tttatggccg gcgcaagcat gtcgtcaacc tctatgtttg gccgaacccc
     1261 ggccatgccg accagccacc gaagccatct agcgtgaatg gctacaacct tatcgggtgg
     1321 caaagcgacg gcatgcggtt atcggcaatc tccgatctcg ctgccggaga attagcggaa
     1381 tttgcccgcc agttcatcat gaaatcggca agcgaagcac ccaactcctg agcgctggcg
     1441 ttatttcgcg aggggcagcg gcttgtccaa ggcaataact aggatctggc tcaccttgcc
     1501 cttgcctatg ttattaccct gctcgtcata cttgctttct tcactgtcga aatcgttatc
     1561 gttggcaact gcgatagtgt tgcggtcgat gacggtgacg ccttcgattt tctccggcat
     1621 ttctttgaat tggttcaagt cgagaaccag cgtttttggc aacacgcgaa ttgccgcgct
     1681 ggcgggatcg tccagcgctt ctaagctcgg ggtcgtgctc gcctcgtccc acttggattt
     1741 gagaatattg gtggcctgag tcagatcgac gctatacagc ttggctgacc aatccgtccg
     1801 ctccaagacc agcagcgtgg tcggattgag atataccacc gccgacagtt tcatttcgtc
     1861 cgggctcatt ttgggccggg gatcgaaatc tttggcaact tcaaaacgat aaacatattc
     1921 cgcggtgacc tgctcctggt cgcgatcgaa gaccaccaca cgggtgttgc gcgaggcatc
     1981 gccgatcttg cgatcgggat tggccagcgg gctttgcagc acggcataga gagtcttttc
     2041 atcggcgctc accgccagcc cctcgaagcc gcggttgatt tttcgtttgc cgaaaatcgc
     2101 cggcaacgat tcctggactg gataatccgc gccgggcagc ttgacacccg ccggtacgta
     2161 acgcttgatc accttgccgg ttttgtcggc gtgaatcagc gacgggctat actcatcggc
     2221 cagccagaat tcgccggatt tcgttcggat caatccttcg gtatcgaggc cattgacgtt
     2281 gaaaccaaac tccttctggg ccgtgtaatc atagggagtt tcatcgatgg ttctggcatt
     2341 cggcaggccg gtgatcggct tgcctgaacg gccgacaatc ggcaccgtct cgacgatttt
     2401 gatcgcgccg ccttccaact tcactcgaac gatggtggga ttaaactcgg gaacccagaa
     2461 tgtccgtcga ttagcgccat cgacgcggat ctggccattg gggccgcggt cggtgaccat
     2521 ccagaactcg ccggcggcat cctctgcgcc gcgccacaga tcgctgccaa ccgagcccaa
     2581 cagcaccttg cgattattag cgacgctgcc ggcaaaccta gcgttctgaa aatccttgat
     2641 ggatattggc ggaaactggg agatcccgac aatccgccct tgggcaaaag cttgatcaac
     2701 cccaggggat aaacatgcgg cgatacccac gcaaaccatt agccagcgaa ctcggacaga
     2761 aaataaagtc atagaaacgc gtacctccct gctgcaggtc cccggaaggt atcggcggtt
     2821 ggtaaactga cggttaaaaa taggttaagg aacggttacg aaatcgaagc cgatcggctt
     2881 tagcgcggag ggtttgtgcc ccgtgcccgc aattgtcatt ctcgagcgcc atcggagatt
     2941 attgcatgcg taattctttc tgcacttcgc gcaatagcga tagatcgcgc acctgcgaca
     3001 atgggatcgg cttatccgtg cgcaccgtcg ccttgcgcga ctcgatggcg aattcgtagg
     3061 tcttgtcgac ggtgctgccg tcgaggctga acgagggcag gatcgaatcg taggagtcgc
     3121 gcgcgacgtc ggcgctttgg ctcagccatt ttcccatgat cgccagggta tcgtcgcgcc
     3181 gctcatgcat gactctcaga ccgcgcagca tcgcccgcag cactcgtttg gtttcctcgc
     3241 ggtggctttt tagacgaacg tcggtaaccg ccacggccga gatcgggctg ccgatcttca
     3301 agtccttcgg cccgccgaga acattgtaac cttcgcgcgc caagcgcacc ggacccggcg
     3361 gcacggtgca gatggcgtcg accagccctt tgcgcagcat ttccgtgcgc accggttcgt
     3421 cgccgccgag ggtgacgaac tgcacgtctt taacgtcgat ccccttggcc tggaaaatcg
     3481 cttccgccgc cagctgatcc gagccaccga tgcgctgcga gcccatgcgt ttgccgcgca
     3541 tgtcgccgat ggttttgatc tcggggcgcg cgacgatgct atgcagcggt ttatcggtca
     3601 gcacgccgag aattttaaat ggcaatcccg ccatggcgct gccaataatg ttgccgaagc
     3661 tgagggtata gtgcacctcg ccgctgagca gcgccggcac cgtcacttgg ctgcgcattt
     3721 gaatcagcag cggctcgaga ttttcatcct tgaagaagcc tttgaccacg gcgatctgcg
     3781 ccggcaggaa agcatagctg cggctgctgt aggaaataat cactttatcg gcggcgagcg
     3841 ccggcaccga cagcaggacg gcaacaatga gtgcgaaaac attaagttgg tttttcatgt
     3901 ttttcctatc cacaaatcga tggtgcgaaa atcgatttcc gcttcgaacg aatccgtcaa
     3961 cgccgctccc gatccttgga atggcaactc accgagacag gcgacgccag tcaaacccag
     4021 cagcacttcg cgattcgtct cagcggctaa agaatcttcg ttggacagtc gattcaaaac
     4081 atagccgagc aagcgcaatt ctttgcagct ggcatgttcc aaggtgagca gcagatgatt
     4141 gatcgcgccg agccggttgg ccgcgacgac gatgaccggc aatttagcga cccggacgaa
     4201 atcagcaaag gtgtagctcg gcaagagcgg caccatcagt ccgccggcgc cttcgaccaa
     4261 tgtaatgtcg tgagccgcct tgatctcgtc gaagaccgcg agcaagtggt cgatgtcaat
     4321 tcttgttccg gcgcgctcgg cggcgatact tggcgccaag ggttcgggca aagggtatgg
     4381 gcataccttc tcgatgggca cggcgcactc ggacgctttc ttcagctgcc atgcgtcctc
     4441 gggatagagc atgccgttgc gcatgacgca gccggtctcg gccggcttca tcacaccgac
     4501 ccgatagccg tagctcctca acaaccgcgc caggccgcag gtaaaaaacg ttttgccgac
     4561 cccggtgtcc gtgccggtga tgaagatcgc ttggcccatt tattttcccg tcaccgtgac
     4621 gatgcagtcg tggac
//
LOCUS       DASQKS010000682         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_6287483, whole genome shotgun sequence.
ACCESSION   DASQKS010000682 DASQKS010000000
VERSION     DASQKS010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6287483"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VE170_17685"
     CDS             <1..546
                     /locus_tag="VE170_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HYJ17330.1"
                     /translation="PIQSAKDVPSPYDIYRKAVAVDAWSENDDEVTLPDHDRGLDFGT
                     SVRAGAEAVTGFGRLKTYLWAFTLQPALEFVLTQGPVVLGINWYSSFVPDAEGIITIR
                     PTARVAGGHALLWRGADTKRGLALLSNSWGDTWGKSGECFIPLRDLDRLIALEQGEAC
                     TAIEQRLRASVALPPPARAAA"
     gene            complement(852..1844)
                     /locus_tag="VE170_17690"
     CDS             complement(852..1844)
                     /locus_tag="VE170_17690"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17331.1"
                     /translation="MKLKIAFQHLLLCSLMTTAVSAFGAQPVTKIIMTSGSFSERDAV
                     NYIAQDQGFFRRYGLEMTFVIVRNGPVGMAALASGETQLHSGSATGAVLGSAAEGMDL
                     VFVAGIINKLIGNIMASPKIKTPADLRGKTIAVTSASGGSWMFTTLALEYWGLDAKRD
                     GITFRILGDEAVRSQALFNDTVAATHLGYTFSAPLISKGFTNLGDLAKLPIPFQSTGV
                     LTSRRYMNSSPEIVENVLRALVDSLHFIAQPENKPAVLKSLMKGLRLKSVDQATEGYD
                     TLAGIYERKIYPRADGVRNVIRLLGQTNEKIRKLKAEELVDDRFVRKLEKEGRF"
     gene            1959..2777
                     /locus_tag="VE170_17695"
     CDS             1959..2777
                     /locus_tag="VE170_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ17332.1"
                     /translation="MIPDPEKFRNFELAGWQEIPAGYHDAFGPLTRQAIAPLLKAVRL
                     KKGMSFLDIASGPGYVAAAAAKRGATVLGIDFSAAMVAHAQRSHSSVEFREGDAEELP
                     CANGLFDAAAMNFGILHLGQPEKALLEARRILRAGGRFAFSVWAKPEETIGFGIVLRA
                     VELHGEPRVELPEGPPFFRYSDPDECTRGLIVAGFESPAVTRVKQLWRLPAGDGLFDV
                     MKDSTVRTAGLLRAQKPAVLEKIREEMRGASAKYTKGGVVELPMPAWVASGVKS"
     gene            complement(2965..4572)
                     /locus_tag="VE170_17700"
     CDS             complement(2965..4572)
                     /locus_tag="VE170_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYJ17333.1"
                     /translation="MDRAPNFLFIITDQHRADHLGCYGNPIVKTPSIDRLAQAGTRFD
                     NFYVATPICMPNRATFMTGRMPSLHGARQNGIPLALTATTFVELLRAAGYDTALIGKC
                     HLQSISGARPTIGMPEADPDRIQPPEHLREADNSWLNHGRYDQELRATWQNDANFELT
                     LPYYGFNQVELAVGHGDEVVGHYDRWLKQRAVDGEALRGRKNQLPGNNYIAPQAWRTA
                     IPEELYPSAYVAERTIKYLENYSRSDRARPFFLQCSFPDPHHPFTPPGRYWDMYDPTD
                     IPLPPSFNPGERPIVPHLRRLYQERTAQEANKDGQRTFAVTEREVREATALTYGMISM
                     IDDAIGSILSWLKTLRLDDDTVVIFTSDHGDFMGDHQLLLKGALHYRGLVRVPCIWSD
                     PQIQAPGKLNAGLCGTIDLAPTILQRAGLAGHNGMQGKSLISALDGGITGHESMLIEE
                     HQRRGYMGLANNFRARSLITRQHRLTIYEGVGWGELYDFSSDPHELDNLWNERGAQPL
                     RQELTEQMARKLIEMTDTSPLATHHGP"
     gene            4693..5208
                     /locus_tag="VE170_17705"
     CDS             4693..5208
                     /locus_tag="VE170_17705"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYJ17334.1"
                     /translation="MIDAAVLIPVYRSQPGDLHIVMILRNPGGVHGGQIAFPGGKHDP
                     ADDTMLDTALRETREELGLVIPRSDILAELPMEQTRTTGYRVFPYVARITLPDRWQIA
                     EREIAEIVDVRLSDLTSPGAHDKMIERFPTWQKAEQVAYYRIGEHRLWGLSYRILQPI
                     IPRLVGGDWNV"
BASE COUNT         1073 a         1466 c         1678 g         1207 t
ORIGIN      
        1 ccgatccagt cagcaaagga tgtgccgagc ccgtacgata tctaccggaa ggcggtggcg
       61 gtcgatgctt ggtcggaaaa cgacgacgag gtaactttgc cggatcacga tcgaggcctg
      121 gacttcggta ccagcgttcg cgccggcgcc gaggcggtga ccggcttcgg gcggctcaag
      181 acttatcttt gggcgttcac cctccagcct gcgttggagt tcgtattgac tcagggaccg
      241 gtcgttttgg gcatcaactg gtacagcagc ttcgttcccg atgccgaagg gattatcacc
      301 atccgtccca ccgcgagagt ggccggcggc cacgcgcttc tgtggcgcgg tgccgacacc
      361 aagcgcggtc tcgcattgct gtcgaattcc tggggcgata cctggggcaa gagcggcgaa
      421 tgctttatcc cgctgcgtga tctcgaccgg ctgatcgcgc tcgagcaggg tgaggcgtgc
      481 acggcgatcg agcaacgcct gcgggccagt gtggcactcc cgccgccggc gcgagcggcg
      541 gcgtgaggtt ctcctgttgt tgtttggctt gcggacatgt gggcgcggtg aatctgcaac
      601 cgttctgtct aatatttcaa ggtaggaaac taaggacccg tgaggcagcg ccggtgtgca
      661 tcagacgcgg gccttgcgca aattctagct aaacggcatt cgtggcaggt cgcacgacga
      721 ctcgccacgg ttagtcacgt tcaagcctcg tttgtagatg tgcgcaatta aaatgcactc
      781 ttgtcgatgt cgcggccgtg acccgaacgg tcggtgaatc gcgcaaaagg ggtgcgatcg
      841 gctgggccgt tttagaatct gccttccttt tccagcttcc tgacaaagcg gtcgtcgact
      901 aactcttctg ctttgagctt gcggattttt tcgttggtct ggccgagcag acggatgaca
      961 ttgcgcacgc cgtcagcgcg gggataaatt ttacgctcgt aaatacccgc aagggtgtca
     1021 taaccttctg tcgcttgatc aacgcttttt agccgcaacc ccttcatcaa acttttcagc
     1081 accgccggtt tgttttccgg ttgggcgatg aagtgcaacg agtcgactag agcgcgcagc
     1141 acgttctcaa caatctctgg agacgagttc atatagcgcc ggctcgtcag gacacccgtg
     1201 ctttgaaagg gaattggcag tttcgctaag tcgccgagat tggtaaatcc tttgctgatc
     1261 agcggcgccg agaaggtgta gcccagatgt gtcgcggcga cggtatcgtt gaacagcgct
     1321 tggctgcgca ccgcttcgtc gccgaggata cgaaaggtaa tgccgtcgcg tttcgcgtcc
     1381 agaccccaat attccagcgc caaggtagta aacatccagc tgccgccgct ggcgctggtg
     1441 acagcgatgg ttttacctcg caaatccgcg ggggttttaa tttttggcga ggccatgatg
     1501 ttgccgatca gtttattgat gatgccggcg acaaacacca ggtccattcc ctcggccgcg
     1561 gaaccgagca cggcgccggt ggcggagcca gaatggagct gggtttcgcc cgaggcgagc
     1621 gccgccatgc cgaccggccc gttgcgcacg atgacaaagg tcatttccag accgtagcgg
     1681 cgaaagaaac cctggtcctg ggcgatgtag ttgacggcat cgcgctcgct gaacgagccg
     1741 ctggtcatga taattttagt gaccggttgg gcgccgaaag cggatacggc tgttgtcatc
     1801 agactgcaaa gcagcaggtg ttggaatgca attttaagtt tcatagacgg cctccgcaac
     1861 cgacagtaaa gaaaagcgct gacacggtca aggcgaaata cctttacgct tccggccatg
     1921 ctataagacc agcgagattc cagaactgtg aacggaagat gatccccgac ccggagaaat
     1981 tccgtaattt cgaattggcc ggctggcagg aaatacccgc cggttaccat gatgctttcg
     2041 ggccattgac ccggcaggcg attgcgccgc tgctcaaggc ggtgcgcttg aagaaaggca
     2101 tgagctttct cgatatcgcc agcgggccgg gctacgttgc cgcggcggct gccaagcgcg
     2161 gcgcaacggt gctggggatc gatttttccg cggcgatggt cgctcacgcc cagcgcagtc
     2221 actccagcgt ggaatttcgc gaaggcgacg ccgaggagct gccctgcgcc aacggcctgt
     2281 tcgacgccgc ggcgatgaat ttcggcatcc tgcatcttgg tcagccggaa aaagctctgc
     2341 tcgaagcccg ccgcatactg cgcgccggcg gcagattcgc tttctcggtg tgggccaagc
     2401 cggaggaaac catcggcttt ggcattgtct tgcgcgccgt cgaattgcat ggcgaaccgc
     2461 gcgtcgagtt gcccgagggg ccgccgtttt ttcgttacag cgatcccgat gagtgtacac
     2521 ggggcttgat tgtcgccggc ttcgagtcgc ccgcggtgac cagagtgaaa cagctctggc
     2581 ggttaccggc cggcgatggt ttgtttgacg tcatgaaaga tagcaccgtg cgcacggccg
     2641 ggctcctgcg cgcgcaaaag ccagccgtat tagaaaaaat ccgtgaagag atgcgcggcg
     2701 catcggccaa gtacaccaag gggggcgtcg tcgaattgcc gatgccggct tgggtggcgt
     2761 ccggggtaaa gtcttaattc gttcaatcgc tttgctcagt tcaagttgtt caaatcgttc
     2821 aagtcgattc ggccaacgga attttttgca acgccgtaac tctgttggcg gtaatattac
     2881 attggtgtga ttttgtcggt cagttgccgg agaaacgatt tgaacggctt gaacgtttta
     2941 aacgattgga acctgtttgc tcttttaagg tccgtgatgc gtcgccagcg ggctcgtgtc
     3001 ggtcatttcg atcagtttgc gcgccatctg ttcggtcagt tcttggcgca agggttgagc
     3061 gccgcgttca ttccaaagat tgtcgagctc gtgcggatcg gacgaaaaat cgtaaagttc
     3121 gccccaaccg acgccttcgt aaatcgtcaa tcggtgttgc cgggtgatca ggctgcgggc
     3181 gcggaagtta ttcgctagac ccatatagcc gcgccgctga tgctcttcga tgagcatcga
     3241 ttcgtggccg gttatcccgc cgtcaagagc ggaaattaaa cttttcccct gcatgccgtt
     3301 gtgacccgcc agtcccgccc gctgtaaaat cgtcggtgct agatcgatgg tgccgcacaa
     3361 gccggcattg agcttgccag gcgcttggat ctgcggatcg ctccagatac acggtacgcg
     3421 caccaggccg cggtaatgca gcgcgccttt taacaacagc tgatgatcgc ccatgaagtc
     3481 accgtgatcg ctggtaaaaa tcaccaccgt gtcgtcgtcg agacggaggg tttttaacca
     3541 ggacaaaatg ctcccaatgg catcgtcgat catcgagatc atgccatagg tgagcgcagt
     3601 ggcttcacga acttctcttt cggtgacggc aaaggtgcgc tgaccgtcct tattcgcctc
     3661 ctgggcggta cgctcttggt agaggcggcg caggtgcggc acgatgggtc gctcgccggg
     3721 attgaacgac ggtggcaagg gaatgtcggt cggatcgtac atgtcccagt agcgtcccgg
     3781 cggcgtgaac ggatggtgcg ggtcgggaaa tgagcattgc agaaaaaacg gtctggcgcg
     3841 gtcgctgcgc gaatagtttt ctaaatattt gatggtccgc tcggcgacgt aggcggaggg
     3901 atagagctct tccggaatcg cagtgcgcca ggcctggggc gcgatgtaat tattgcccgg
     3961 cagctgattt ttgcgcccgc gcaacgcctc gccatcaacg gcgcgctgct tgagccagcg
     4021 atcgtaatgg ccgacaactt catcgccatg gccgacggcg agttcgactt ggttaaagcc
     4081 gtagtaggga agggtaagct cgaagtttgc gtcgttttgc caagtggcgc gcagttcctg
     4141 atcgtaacgg ccatggttaa gccaactgtt atcggcctcg cgcaggtgct ccggcggctg
     4201 aattctgtcc gggtccgctt ccggcatgcc gatggtgggg cgtgcgccgc tgatactttg
     4261 caggtggcat ttgccgatca gcgcagtatc gtaacccgct gcccgcagga gctcgacgaa
     4321 tgtagtcgcg gtcagcgcca gcggaatgcc gttttgccgc gcaccatgca gcgacggcat
     4381 gcgcccggtc atgaatgtcg cgcgattggg catgcagatc ggcgtggcga catagaagtt
     4441 gtcaaagcga gtccccgctt gtgccaatcg gtcgatgctc ggggttttga cgatcggatt
     4501 gccgtagcag cccagatgat cggcgcgatg ttggtcggtt attatgaaaa gaaagttcgg
     4561 cgctctatcc atgggtgatc ctcgacatcg aatgtcttta tcatatcgct gccgctgtgc
     4621 aattgttgaa agcccgcgaa gctttgccac gagcctggga attgtagtag aagcgaacct
     4681 aggacaatca ccatgatcga cgccgccgtg ctcattcccg tgtatcgttc gcagcccggc
     4741 gacctgcata tcgtcatgat cctgcgcaat cccggtggtg tgcatggcgg tcagatcgcc
     4801 tttcccggcg gcaagcatga tccggcggat gacaccatgc tcgataccgc gttgcgtgaa
     4861 acccgtgaag agctgggctt ggtcattccg cgcagtgaca ttttggctga gttgccgatg
     4921 gagcagacgc gcactacagg ttatcgggtg tttccctatg tggcgcgcat cactctgccc
     4981 gaccggtggc agatcgccga gcgtgagatt gccgagatcg tcgatgtccg gcttagcgat
     5041 ctcactagtc ccggagccca cgacaagatg atcgagcgct ttccgacctg gcagaaggcc
     5101 gaacaagttg cgtattaccg aatcggcgaa catcggctct ggggattgtc ctatcggatc
     5161 ttgcagccga ttatcccgcg gttggttggc ggcgattgga atgtctgatg aataacgcag
     5221 aagcctcggt gcctcacttg tgtgtgcgag gttgggggta ttaagtaaaa ctggattcaa
     5281 aaaaggagat taccaatgga tttcaattta caaccggata gccgggagat tagcgcgttt
     5341 cgtcaaacgg tgcgcgcctg gttggcggaa aatatgaagg gtagcgagca tctgcgctgg
     5401 tcgggtaact ggtcgacccg ggaa
//
LOCUS       DASQKS010000683         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2060867, whole genome shotgun sequence.
ACCESSION   DASQKS010000683 DASQKS010000000
VERSION     DASQKS010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060867"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VE170_17710"
     CDS             complement(<1..312)
                     /locus_tag="VE170_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17335.1"
                     /translation="MASKAAGWRTTLSVLILFTMKALLTCIAFALGIECAAAQAQTNE
                     LRFVTWRAEAPQVWDQGIADFERQNPGVKVIREIGPQSSTQLHDLLAQKLKNRDTRLD
                     VF"
     gene            complement(447..1286)
                     /locus_tag="VE170_17715"
     CDS             complement(447..1286)
                     /locus_tag="VE170_17715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17336.1"
                     /translation="MFIIDSQVHIWAPETPEKPYAKENASAPHRAVPLGHDELLREMD
                     GAGVARCILVPPTWEADRNDTSLEAARLHPDRFRVMGRLALTDPNGPALMAAWKQQPY
                     MLGIRMVFNRGQSAQWLNDGTADWFWDAAEKYDIPVMTLAPADVPKLGEVAERHPGLR
                     LIVDHMGLNSVLRGKPLDPSIAAVIKLARHKNLAVKVSALPCYVDEAYPFPTLHPLVR
                     RVVDAFGPQRCFWGTDLSHLPCPYKQVVTLFTEEMKSLSSIELDWIMGRGLAEWLNWP
                     LPK"
     gene            complement(1449..2876)
                     /gene="hemG"
                     /locus_tag="VE170_17720"
     CDS             complement(1449..2876)
                     /gene="hemG"
                     /locus_tag="VE170_17720"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HYJ17337.1"
                     /translation="MKRIIVIGGGIAGLAAAHRIDELARSNGRSIDIKLLEASGRLGG
                     AIATERVGEFLVEAGPDSFISEKPWALKLCDRIGLGHQLVSTQSAYQKIFIVHHGRLV
                     ALPEGFFLLAPTRLWPFIQTPLFSWPGKLRMAAELFLPRGELPDDESLGAFVRRRFGR
                     EALERVAQPLLGGIYAADPDRLSLTATMPRFKDMERERRSVIYAMWSAQRQRAKKSET
                     GSGARWSLFVTLAGGMQELVDALNAGLPAGSVRLNCAARALTLSADQKNWQVAVGDDE
                     AINADAVILATPAFRAGQLLNPIAAGAAVDLTNISYASTATINLAYRREDFPQAPDSF
                     GFVVPAIEKRKIMACTFSSLKYPGRAPENCILMRAFVGGSLQPHQFDESDADMERTVR
                     DELASLLGVTAAPIFSRIWRHLTSMPQYHVGHEALVKRIEGALQPFPTLSLAGSAYHG
                     VGISDCVRTGEAAAEKVFARLLATL"
     gene            complement(2925..3860)
                     /gene="hemH"
                     /locus_tag="VE170_17725"
     CDS             complement(2925..3860)
                     /gene="hemH"
                     /locus_tag="VE170_17725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYJ17338.1"
                     /translation="MPHPYQAILLIAFGGPTRAEEIRPFLARVTHGMQIPPARLDEVV
                     RHYEAVGGKSPLNEITFRQADSLKTFLNNKLPVYVGMRNAAPYLVDTLKLMASAGVAR
                     ALGFILSSHRSEASWERYQRNIADARAELGGSAPEVDYCDGWHDHRLFIQSWVEQIQT
                     AMTKIEAGKRQSTALVFTAHSLPAAMADRSPYVEQLTNSARLIAAALNHSRWSLAYQS
                     RSGRPGDPWLEPDIGQTIRELAAHGDKRIIVAPIGFVCDHVEVLYDLDIEAKTIADGL
                     GVELIRTSCPNDHPTFVRMMADVIDRQLKKAAARE"
     gene            complement(3976..5010)
                     /gene="hemE"
                     /locus_tag="VE170_17730"
     CDS             complement(3976..5010)
                     /gene="hemE"
                     /locus_tag="VE170_17730"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HYJ17339.1"
                     /translation="MATTIHPFLAACRREPTSFTPVWLMRQAGRYMEEYRKLRAQHGF
                     LELCKKPDLATEITVTPVEKLHVDAAILFADILLILEPTGVGLEYSKGDGPVIHRPVR
                     SGKDVDGLNEFDVGSELAFVYETVAKTRKELKNKVPLIGFAGAPFTLASYLIEGSGSR
                     NYVHTKKLFYSAPEAWKRLMERLAKLIGEYLNCQIAAGADAVQIFDSWAGCLTPSDYE
                     QFVQPYTKAVIDAVTPGVPVINFSTGTSGLLKQLRATGGDVIGLDWRINLDEAWTTVG
                     HDVAVQGNLDPVALFAAPKEIKSRVAEILRRAGGRPGHIFNLGHGVLPETPVDHVIAM
                     VDAVHELSAR"
BASE COUNT         1043 a         1426 c         1613 g         1056 t
ORIGIN      
        1 aaaaacgtcg agtcgggtat cccgattttt caatttctgc gcaagcaggt catggagctg
       61 tgtcgatgat tgaggtccga tctcgcggat caccttaacc ccgggatttt gccgctcgaa
      121 gtccgcaatg ccttgatccc aaacctgggg agcttcagcg cgccaagtga caaagcgcag
      181 ttcgttggtc tgagcctggg cggccgcgca ttcgatgccg agagcgaacg cgatgcaggt
      241 taacagtgct ttcatggtaa acaggatcaa caccgacaat gtagtccgcc atccggcggc
      301 tttcgacgcc acgccgcgag cgccatggcg ccaaggttag caattattca tggcaatgaa
      361 ttcaataaaa agcaggcaag tgagatctcg gggccgagga ttcgaccaag tgcaaacggc
      421 gacgcttcga aacaaaagca agcaagctat ttaggcagcg gccaattgag ccattcggcc
      481 agcccgcgac ccatgatcca gtccaactcg attgacgata gcgacttcat ttcttcggtg
      541 aacaacgtca ccacttgttt gtacggacag ggcaagtgcg acaaatcggt gccccaaaaa
      601 cagcgttgcg ggccgaaggc gtcgacgacg cggcgcacca gagggtgcag cgttggaaaa
      661 ggataagcct cgtcgacgta gcaaggcaac gccgagactt taaccgcgag attcttgtgg
      721 cgcgccagct tgatcacggc cgcgatcgaa gggtcaagcg gcttgccgcg cagcaccgag
      781 ttcaacccca tatggtcgac gatcaaccgt aggcccggat ggcgttcggc gacttcacct
      841 agcttgggca catcggccgg tgccaacgtc atcaccggaa tgtcgtattt ctcagcggca
      901 tcccagaacc agtcggcggt gccatcgttc aaccactgcg ccgactggcc gcgattgaaa
      961 accatacgga tgcccagcat gtacggttgt tgtttccagg cggccatcag cgccgggccg
     1021 ttgggatcgg tcaatgcgag tctgcccatg acgcggaagc gatcgggatg cagccgtgcc
     1081 gcttccagtg aggtatcgtt gcggtcggct tcccaggtgg gcggcacgag aatgcaacgc
     1141 gcgacaccgg cgccgtccat ctcgcgcaac agttcgtcgt ggccgagggg cacggcgcgg
     1201 tggggcgccg aggcgttttc tttggcgtat ggcttttccg gggtctctgg agcccagata
     1261 tgaacttgtg agtcgatgat aaacatggcg cgcgcagttc gtcttattaa tgagtcgcta
     1321 gccgattcat agtccggcac tcgtgcgggc gtcaagagaa agcggcaaag atttcctgct
     1381 gcgcttgaaa tgaccgtcga cggccaatga atggacggtt gttatgcggt tgtcgggtgc
     1441 acggcgctct acaaggtcgc gagcagccgg gcaaaaactt tttccgcggc cgcttcaccg
     1501 gtgcggacgc agtcggaaat acccactcca tggtaagcgc tgccggcgag gctgagagtc
     1561 gggaacggtt gcaatgcgcc ttcgatacgc tttaccaaag cctcgtggcc gacatgatat
     1621 tgcggcattg acgtgaggtg gcgccagata cgcgaaaaaa tcggtgcggc ggtgaccccg
     1681 agcaggctgg ccaattcatc gcgaacggtt cgttccatgt ccgcgtcgga ttcgtcaaac
     1741 tggtggggct gcaagctgcc gccgacgaag gcgcgcatta aaatgcaatt ctccggcgcg
     1801 cggccggggt atttcagact gctgaatgtg caggccatga tcttacgctt ctcaatggcg
     1861 ggcacgacga agccaaaact gtccggcgct tggggaaaat cttcgcggcg gtaggcgagg
     1921 ttgatggtgg cggtggaggc gtatgagatg tttgttaaat cgaccgccgc gccagccgcg
     1981 atcggattga gcaattggcc ggcgcggaac gctggcgttg cgagaattac tgcgtccgca
     2041 ttgattgcct cgtcgtcgcc gacagcgacc tgccaattct tctgatcggc actgagcgtc
     2101 aatgctcttg ccgcgcagtt caagcgcacg ctgcctgcag gcaaacccgc gttgagggcg
     2161 tcgacgagtt cctgcatacc gccggcgagg gttacgaaga ggctccaacg cgcgccgctg
     2221 ccggtttcgg atttcttggc gcgctggcgc tgagccgacc acatggcata gatgacgctg
     2281 cggcgctcgc gttccatatc cttgaagcgc ggcatggtgg cggtgagact tagccggtcg
     2341 ggatcggcgg cgtaaatgcc gccgagcagc ggttgagcga cccgttccag ggcttcgcgg
     2401 ccgaaacggc ggcgcacgaa ggcgccgagg ctttcgtcgt cgggcaattc gccgcgcggt
     2461 aaaaacagtt cggccgccat gcgcagcttg ccgggccagg aaaacagcgg agtttggata
     2521 aacggccaga ggcgcgtcgg cgccagcaag aagaatcctt cgggaagcgc gaccagtctg
     2581 ccgtggtgca cgataaatat tttttggtag gccgactggg ttgaaaccag ctgatgacct
     2641 agaccgatcc gatcgcacaa tttgagcgcc cacggtttct cacttataaa cgagtcgggg
     2701 ccggcttcga cgagaaattc accgacgcgc tcggtagcga tggcgccgcc gagccgtcct
     2761 gaggcttcga gcagctttat gtcgatgctg cgcccgttag agcgggcaag ttcatcgatt
     2821 cgatgagcgg ccgctaggcc ggcgataccg ccgccgatga cgattattcg tttcatgaga
     2881 accaggatcg aggatggagg atcgaggatg ggttcgtcga ttctctattc tcgagctgcg
     2941 gcctttttca gttgacgatc tattacatcg gccatcattc gcacaaaggt cgggtgatcg
     3001 ttcgggcagc tggtccggat cagttcgacc cccaagccgt cggcgatggt ttttgcttcg
     3061 atatccaagt catagagcac ctcgacatgg tcgcaaacaa agccgatggg cgcgacgatg
     3121 attcgcttat cgccgtgcgc ggccagttca cggatcgttt gaccgatatc cggttcgagc
     3181 cacggatcgc ccggcctgcc gctgcggctt tgataggcga gcgaccagcg cgaatggttt
     3241 agcgccgccg caatgagccg agcgctattc gtcaactgtt cgacgtaggg agaacggtcc
     3301 gccatcgccg cgggcaggct gtgcgccgtg aaaactaacg ctgtcgactg tcgcttaccc
     3361 gcttcgatct tcgtcatggc cgtttggatc tgctccaccc agctttgaat aaatagccga
     3421 tggtcatgcc agccgtcgca atagtctact tcgggggcgc tgccgccaag ctcggcgcgg
     3481 gcatcggcga tatttcgctg atagcgctcc cagctcgctt cgctgcgatg tgaggatagg
     3541 atgaagccga gcgcgcgcgc gacgccggca gatgccatca gtttcaaggt atcgaccaag
     3601 taaggcgccg cgttgcgcat gccgacgtat accggcagct tgttgttcaa aaaagtcttt
     3661 aatgaatctg cttggcgaaa ggtgatctcg ttgagcggcg atttgccacc gaccgcttcg
     3721 tagtgacgga caacttcgtc aagacgggcg gggggtatct gcataccatg ggtcacgcgg
     3781 gctaggaacg ggcgaatctc ctcggcacgc gtgggaccgc cgaaggcgat gagcaggatg
     3841 gcctggtacg gatgcggcat gggaggtcaa tcgaatgttg gagtgttgga gtattggcgt
     3901 agtgggacgg acggggaggt cttcgaattt aacatcatcc cgacactcca gcactccatc
     3961 ttgccaacgc gtcttctacc ttgcgctcag ttcgtgaacc gcgtcgacca tagcgatgac
     4021 gtgatcgacc ggcgtttccg gcaggacgcc gtggccgaga ttgaaaatat gacctggacg
     4081 gccgccggcg cggcgcagga tttccgcaac gcggcttttg atttctttcg gcgcggcaaa
     4141 cagcgcgacc gggtcgaggt tgccttggac ggcgacatcg tggccgacgg tggtccaggc
     4201 ttcgtcgaga ttgatgcgcc agtcgagacc gataacatcg ccgccggtgg cgcgcagctg
     4261 ctttaataat ccagaggtgc cggtgctgaa gttgatcacc ggcacaccgg gagtgaccgc
     4321 gtcgatcacc gctttggtgt agggttgaac aaactgctcg tagtcggagg gagtcaggca
     4381 gccggcccag ctatcgaaga tctgcacagc gtccgcgccg gcggcgattt ggcagtttag
     4441 atattcgcca atgagtttcg ccagccgttc catcaaacgc ttccacgcct cgggtgcgct
     4501 gtagaaaagt ttcttggtgt gaacatagtt gcgcgagccg ctgccttcga tcaagtagga
     4561 cgccagggtg aacggcgcgc cggcaaagcc gatgagcggc actttgtttt tcagttcttt
     4621 gcgagtcttt gcgaccgtct cgtagacgaa tgcgagttcg ctgccgacgt caaattcatt
     4681 caatccgtcg acatccttgc cgctgcgcac cggccgatgg atcaccgggc cgtcgccttt
     4741 ggaatattcc aagccgacgc ccgtcggttc gaggatcaac aaaatatcgg cgaacaatat
     4801 cgccgcatcg acgtgtaatt tctcgactgg cgtgaccgtg atctcggtgg cgagatcggg
     4861 cttcttgcac agttcgagaa agccatgttg ggcgcgcagc ttgcggtatt cttccatgta
     4921 acggccggcc tggcgcatga gccagacggg agtgaatgaa gtaggttcgc gccggcaggc
     4981 ggccagaaaa ggatggatcg tggtagccat gatgttggtc gagttggaaa acagtatgaa
     5041 gctttaacgg caaaatcaac ttgagcgttt gcgcggcgtc gccgcgccga tggcctggtc
     5101 gccgtacttc cggcgcagtt tgtcgatgcc ttgataca
//
LOCUS       DASQKS010000684         5282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3774643, whole genome shotgun sequence.
ACCESSION   DASQKS010000684 DASQKS010000000
VERSION     DASQKS010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774643"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VE170_17735"
     CDS             <1..819
                     /locus_tag="VE170_17735"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ17340.1"
                     /translation="VIVASGGQMKILGDMYAFGLLGAFAFSSLSLDSIRWKLGRRDLG
                     FWIGVFTTLMVALAWAINIIEKPLATYFGGAVTVIGMLTAVGVRRAWFLDILVQIPFI
                     QRLQARAYHASEDLVEDELKGLMTLSEAVDVKPLYSSSTLLALRDESPGLVQEAITRV
                     RGKGESALYCIYVEEWPGLFAGETPHAPNEQGVQTLKAVLQAVRDKGIEILPIWSVSH
                     NAAEAIANAAKALDVDSVIIGASRRSAFYHMLRGHVIKGLMKKLPRDCHLMICN"
     gene            837..1802
                     /locus_tag="VE170_17740"
     CDS             837..1802
                     /locus_tag="VE170_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17341.1"
                     /translation="MTEQIEMKLRDRAEGSRLMKQFLCSSCILLFSFLAAIAAADFTG
                     RGHVHTLSETKQVFLNPDCQSTDSCDLKRFTLSKAVNEIWFSDDPKHPTYGNGVIMEY
                     ETDSVAAIERYAIVQFKKGCVFNTTQNPQGKIQRIVTDTVPSFGEQVLFCFQQWVIDS
                     HDTDPAYNSDPDYGRFYFLRWNKRGSYDNRTQKYYGAVKPHAPVVYMADYPAGAFVTG
                     TGVRNVALEFKTCIYKASDVPSVSRRENLDFAKPINCFAWENVYVYDFGNAKFQTDLA
                     SVPRWEESGAPVNDYRRVVGVALLIVLAMFILARVMTLYGRQTSR"
     gene            complement(1946..2905)
                     /locus_tag="VE170_17745"
     CDS             complement(1946..2905)
                     /locus_tag="VE170_17745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17342.1"
                     /translation="MARKATYSRRSGASGAYRAAFSEVHGEPFPTPGGSDHDVDARIR
                     DMDEEGVDVQLMLPGLPPATDDVDIEIAFIRANHRFTHEFCSEHPHRLKSLIVVSSRA
                     VIDSVQEIKTWSRAPWAAGIHPNLAEGIPIDHPDLDPIWAAANDAGLCVAHHSFTAGY
                     PGYRDLWDNPFIGRTASHPWGAMRAVSAFFGAGIMDRYPNIRYCILESGFGWLPFWAA
                     RMDDQCEYMAYAMPENLARKPSEYMTSGRFFASLVIHEGERMARMVTDFLGDGVLMYS
                     SDYPHAESRFPESTNKVLAWKSLKDPIMRKILWDNATKCFGAP"
     gene            complement(3337..4356)
                     /locus_tag="VE170_17750"
     CDS             complement(3337..4356)
                     /locus_tag="VE170_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17343.1"
                     /translation="MHFTGKHGIIYASVFILVTLAMLSAPRAQDKLLISYGGHNETVG
                     PMWVAVDKGIFRRYSLDVNMLQVRNGQISLTAVMAGDVQAFWPAVSSVISGVSGGAKL
                     GCVASPFNKIARELIVRAEIASLAALREKIFGVQSIGGGFWLQTMIVLDRLGVDADKY
                     KLQMRVIGDEPTILQALLASNIDAAVITQASAAVAKRAGLRSLANTTELKVPYQGVGI
                     CTRSDRIANSPDLIMRLVKGMVEGVVFIQSPRNKREVTDVLKKHLRLSTDQDAETSYN
                     ALRTVSSLDVAPDPEAWKNIQKYVARVTPKVGQLDIHQIINGNFVKTLEETGFVAEAK
                     KRHGQ"
     gene            5084..>5282
                     /locus_tag="VE170_17755"
     CDS             5084..>5282
                     /locus_tag="VE170_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17344.1"
                     /translation="MQKINVLAAVALISLFIGSPHRLQAQSSFYRDKTITIIVGTVPG
                     GLYDLWGRLFSRIMGKYITGNP"
BASE COUNT         1174 a         1486 c         1426 g         1196 t
ORIGIN      
        1 gttatcgtcg cgtcgggcgg acaaatgaaa atcctgggcg atatgtatgc cttcggacta
       61 ctcggcgctt ttgctttttc ttcgttgagc ctcgattcca ttcggtggaa gctcggccgt
      121 cgcgatcttg gattctggat cggcgtgttt acgacgttga tggtcgcgct ggcctgggcg
      181 atcaatatca tagagaagcc gctggccact tatttcggtg gcgcggtcac agtgatcggc
      241 atgcttaccg ctgtcggtgt ccgtcgtgcc tggttcctcg acattctggt gcagattccc
      301 ttcatccagc gtctccaagc gcgcgcctac cacgcctcgg aagatctggt ggaagatgag
      361 ctgaaaggat taatgacact ctccgaggcc gttgacgtca agccgctgta ctcgtcgtca
      421 accttgttgg cattgcgtga tgaaagcccc ggtttggtgc aggaagcgat cacccgtgta
      481 aggggcaagg gagagtcggc gctctattgc atttacgtgg aagagtggcc gggtttgttt
      541 gccggggaaa cgccccatgc gcccaatgaa cagggagtcc aaactctaaa agcggtgtta
      601 caggccgtcc gtgataaagg aatcgagatt ctgccaatct ggtcggtgtc gcacaacgcc
      661 gccgaagcga tcgccaatgc cgccaaggcc ctcgacgtcg attccgtgat catcggcgca
      721 tcgcgccgat cagctttcta ccacatgctg cgcggccatg tgatcaaagg attgatgaag
      781 aaattgccga gagactgcca tctgatgatc tgcaattgag atgaagtcga aggatattga
      841 ccgagcaaat tgagatgaag ttacgggaca gagcagaagg atcccgactc atgaagcaat
      901 ttctctgttc ttcatgcatc ctgctgttct catttctagc cgcgattgct gcagccgact
      961 ttaccggcag agggcatgtg catacgctct ccgagaccaa acaggtcttc ttgaatccag
     1021 actgccagtc caccgacagc tgcgacctca aaagattcac gctgtccaag gcggttaacg
     1081 agatttggtt cagtgatgac cccaagcatc ccacctacgg caacggcgtg attatggaat
     1141 acgagaccga ttcggtggcc gcgattgaaa ggtacgccat cgttcaattc aagaaaggct
     1201 gcgtctttaa cactacgcaa aatccccaag gcaagatcca acggatcgtc acggacaccg
     1261 tgccaagctt cggcgaacaa gtcttgtttt gttttcaaca atgggtgatc gactcccatg
     1321 ataccgatcc cgcctataac agcgatcctg actatggcag gttttacttt ttgcgctgga
     1381 acaagcgagg ctcttacgac aaccggacgc agaaatatta cggcgcggta aaaccgcatg
     1441 cgccggttgt ctacatggcc gactaccctg ccggcgcgtt cgtcaccggc accggcgtca
     1501 gaaatgtcgc gctggaattc aagacctgca tttataaggc aagcgatgtt ccttcggtat
     1561 cacgaagaga aaatttagat tttgccaagc cgatcaactg tttcgcttgg gaaaacgtct
     1621 atgtctacga tttcggaaac gcaaagtttc agactgattt ggcgagtgtt cccaggtggg
     1681 aagaatcagg cgcacccgtg aatgattacc ggcgggtggt tggggtcgca ctgttgattg
     1741 ttctggcgat gtttattctc gcacgcgtaa tgacgcttta cggccgacaa actagccgtt
     1801 aatcaacaat tcgtaggcgc tgggcccttg atcctccgca aggcatgaat cggctgggcc
     1861 gagcgtcgat aataccgaca atagtttctt gccgaacttg gaactgttgt tgcaacgtat
     1921 actcccccga atttccgaaa accttctacg gcgcgccgaa gcatttggtc gcattgtccc
     1981 agagtatttt cctcatgatc gggtctttca gggatttcca cgccagtact ttattggttg
     2041 actcggggaa acgtgattcc gcatgcgggt aatcggacga atacattaac acgccgtcgc
     2101 cgaggaagtc ggtgaccatg cgcgccatcc tctcgccttc gtggatcacg agactcgcga
     2161 agaatcgccc gctggtcatg tattcgctgg gtttgcgagc aagattctcc ggcatggcat
     2221 aagccatgta ttcgcattgg tcgtccatcc gcgcggccca aaacggtaac caaccaaagc
     2281 cggactcgag gatgcagtaa cggatgttgg gataccgatc cattatcccg gcaccgaaaa
     2341 atgcgctgac tgcccgcatc gcgccccacg gatgcgaagc cgtccggccg atgaacgggt
     2401 tgtcccacag atcgcgatag ccgggatatc cggcggtgaa actatgatgc gccacacaga
     2461 gtccagcgtc gttggccgca gcccagatcg ggtccaggtc cgggtggtcg ataggaatgc
     2521 cctcggccaa gttgggatgg atgcccgcgg cccagggcgc ccgcgaccag gttttgattt
     2581 cttgcacgga gtctatgaca gctcggctac tcacgacaat gagagacttc agacggtgcg
     2641 ggtgttcgct gcaaaattca tgggtaaagc gatggttggc ccggatgaag gcgatctcaa
     2701 tatcgacatc gtccgtggcc ggcggcaatc cgggcagcat gagctgaacg tccacaccct
     2761 cctcgtccat gtcccggatg cgcgcgtcga cgtcatgatc cgacccgccg ggcgtgggaa
     2821 acggctcccc atgaacttcg gaaaatgcgg ctcgatatgc tcccgaggcc ccgcttcgcc
     2881 tagaatacgt agcttttcgc gccagccatc tcttttctta aactggtaac ggtggcgata
     2941 tggcgggtcg agaacctcgc cggcccagcc ggtgcgaaac ggcaccttgc aactcgccca
     3001 ttccggtaat cgctggcgca tggccgcatc gaaataaggc tccagagttt cggccaaagg
     3061 attgaggtgc gtgtccgcat cgaaaatctt aaaaccgtcg cgcatgggaa atccttcctt
     3121 gaatgccgac tgggaagcca tccataacac gatcagtggc gctggtgaac aattgtcgga
     3181 ttaagaggca acagcagaag agagcttacg ggataaatga tttctgaccg cgcccgcctc
     3241 gcggcacgac gtgaaattta attacggcac attccgacga catccgcccg cgagatacaa
     3301 aacctggcgc ccctgccccg cgcgcttgtc ttgctgctat tggccatggc gctttttcgc
     3361 ttcggcgacg aagccggttt cttcgagcgt cttgacgaaa ttaccattga tgatctggtg
     3421 aatatccaac tggccaactt taggagtcac tcgtgcgaca tatttttgaa tatttttcca
     3481 agcctcggga tcgggagcga catcgagcga agataccgtg cgtagtgcgt tgtaagaggt
     3541 ctcggcgtcc tggtcagtgc tcagccggag atgctttttg agcacgtccg tgacctcgcg
     3601 tttgttacgc gggctttgaa taaacaccac gccctcgacc atgcctttta ccaagcgcat
     3661 gatcaggtcc ggcgaattgg cgatccggtc gctgcgcgta cagatgccaa cgccttgata
     3721 aggaaccttc agctccgtgg tattcgctag ggagcgcagc ccggcccgtt ttgccaccgc
     3781 ggcagaagcc tgcgtgatca ccgccgcgtc gatgttcgag gcgagtaggg cttggagaat
     3841 cgtcggttcg tcgccgatca cgcgcatctg caatttgtac ttgtcggcat cgacgccgag
     3901 ccgatccaaa acgatcatcg tctgcaacca gaagccgccg ccgatgctct gcacgccgaa
     3961 gattttctcc cgcagggcgg caagcgaggc gatttcagct cgcacgatca gctcccgcgc
     4021 gatcttattg aagggactgg cgacgcagcc gagctttgct ccgccggaga ctccgctgat
     4081 gaccgaagac accgcgggcc aaaaggcctg cacatctcca gccattacag ccgtaagact
     4141 gatctggcca ttgcgcacct ggagcatgtt gacgtcgagg ctgtatctcc tgaaaatgcc
     4201 cttgtccacg gcgacccaca taggaccgac ggtctcatta tgacctccgt aggagatcag
     4261 cagcttgtcc tgagcccgcg gtgccgagag catcgccagg gtcaccaaga taaaaacgct
     4321 ggcgtaaatt atcccgtgct tgcctgtgaa atgcatgttt acctccaaaa aatcagccta
     4381 ttcttcgatt agttccgcgc cccgcctggc gagcgcaacg tcccaccaat tccgcacatc
     4441 gacgcccctc ttagcttgcc ggcacgcttt accaaccgat cattgccgga ttcttagttt
     4501 atcttttcaa atccttcagc aagcaggctc gttattacat atatttcgac gttgcgagaa
     4561 tccaagaaac gtgacaggtc ctcgtcgtta tgaatccgat aaacgatttg aacggagcga
     4621 agcaattgaa cgattggaac gtttggaact gcccgctctg gcaatatccc gaaccacgag
     4681 cacttacacg gccgtttggt tcgtcctcgt ggaactctcc gcacgacggc cttctaccat
     4741 cgcgcccctc ggcgcttcaa tccaatcgaa aagtagcttg tccccttttc ttactttgcc
     4801 tgtcttttct ctccccttgt gccagctaat ctttcgcgct acacgctaaa aacgaggatg
     4861 attcagggag cagcgggcgt acaccacgcc gtcgtaatgg tgcagtcgtc atagaaaacg
     4921 tcacctgcga ccagcgcttg tcctggccgg attctgcggc caaagagcgt ggcagaactg
     4981 acccatgagg ttcgagttcc atgagcgcca aggggttatt ctaatcattc caaatgttcg
     5041 aattaggtga ccggtgggtt tatcagatca caggagacgc agaatgcaaa agattaacgt
     5101 gctcgccgct gtcgcgctca taagcctgtt tatcggctcg ccacacaggc ttcaagcgca
     5161 atcgagtttc tatcgggaca aaacgatcac gatcatcgtc ggcacggtgc ccggcgggct
     5221 ctacgacctc tgggggcgac tgtttagccg aatcatgggc aaatacatta ccggcaaccc
     5281 cg
//
LOCUS       DASQKS010000685         6155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4191743, whole genome shotgun sequence.
ACCESSION   DASQKS010000685 DASQKS010000000
VERSION     DASQKS010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4191743"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1153)
                     /locus_tag="VE170_17760"
     CDS             complement(71..1153)
                     /locus_tag="VE170_17760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17345.1"
                     /translation="MAPRKLLAYSVTSLLLVCSLSSNAHAASVEALYSEINKLPPAQR
                     QKRLEDGARKEGGFKFYGISAATLLGAYTAGFMKRYPFIKAEFWRGSGNKLVFRTLTE
                     HRAGQLDTDAVLVGTENVMALKRSGIWVRYYSPESVNFGKEYTDKDGFWHADSLGVST
                     LGYNTKLVKKEEAPRGYDDLFDAKWKGTLSIDLEPERALMGWLSAWGEQKTRAFVDGL
                     IKHGATVRRGHTLQAQLLCAGEFKVAVEIYPDGLLRMKQGGCPAALVFPNPTPAHISG
                     AIGIYTNTKNPHAAALFLDYMKSAEGAKILASTGRISGRKGVKSLYDELNNLEERGIP
                     LLIVGPEQSELVSKPMEKIMKDILVP"
     gene            complement(1188..2279)
                     /locus_tag="VE170_17765"
     CDS             complement(1188..2279)
                     /locus_tag="VE170_17765"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17346.1"
                     /translation="MADTPVTAEDEVKERVAYVSPYEQWKKDEGLPTYRGLAIKDVNA
                     VELAPWKSSGAGMAAFLNLEGTGGFNDSYIAEIPPGKSLKAHRHLFEETIYILKGQGA
                     TSVWIDPTKKETFEWQEGSYFAIPMNAWHEHHNASGLDSARYIAMTSAPRVIDTFNNY
                     EFVFENPFVFKDRYNSEEGYFKQAAPAQQWATNFVADVRVCETYAGGFDRGGGAKSTV
                     FRMVKQTVKSHVSHWGVGAYKKAHRHGPGIHVLIIRGTGYSILWHENKPLERVEWGPG
                     SVFVPPEMWFHQHFNGSAEPVFFLAIGWGSDKPKAGGKAYVYKSVKEGGDQIEYEDED
                     PVIHAEFETAMKKAGARCKMDYHPHCTMK"
     gene            complement(2409..3089)
                     /locus_tag="VE170_17770"
     CDS             complement(2409..3089)
                     /locus_tag="VE170_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17347.1"
                     /translation="MSHWLEIRPTIDAMVNRQFDTPEFKKLFAVKLTPGRVQVYQNHM
                     TFYANNRRDCWAFVSGKAPLEVKRAIWAHEKDELIHDERMGRAHVTDNDLQLADESQL
                     SPGAKAAHYAWLYCAREKPWLEGLIYSHILERRNNNAIVKGGGLVERWKRKQRAELGV
                     KTAEKDFQTEVHAVADEDHSDIFEPIFLRYVDSEAAVQAVLAATRDGLLIDRALRGAL
                     ADAMLAVE"
     gene            complement(3128..4264)
                     /locus_tag="VE170_17775"
     CDS             complement(3128..4264)
                     /locus_tag="VE170_17775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17348.1"
                     /translation="MATQQFISVDEHVQEHPDVWTKRLSAAKWGERIPHVAKDAQGNE
                     RWLIDGRPIAMDGVADCGAVMAPRTANPTRWADVPAMVYDARQRLRAMDAAGIDHAVL
                     YPTVAGIAGQNFGRIEDGELELACVQAYNDWLLEEWAGVSPRFVPQCLVPLAPIGSTV
                     AEIRRCIANGHRGVVYPSIPMELRDLPHINDEIYDPVWAVCQELGVPLCFHAGASAKI
                     QIPAYAGYPPATAAAIQAITGPASSVSVLVNLLISKILMRFANLKIVLAGSGLGWGAY
                     LLEYTDFQATGDQLPQNGYDLMPSELFKRQCYLVGWYDCASLRVRDYIGAENILWSSQ
                     FPQATSTWPDTRAALAKSFAGVAEADKQKILWQNAAQLYKITVA"
     gene            complement(4384..5535)
                     /locus_tag="VE170_17780"
     CDS             complement(4384..5535)
                     /locus_tag="VE170_17780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17349.1"
                     /translation="MEVKFGLFSCDSHAQLDRDAWTKRMSKAKWGERIPQIVEVDEDG
                     KRVDRWMVNGKVRSEWVCNCPAAMPGGVARGYYPRRWQEVPEIVYDPAARLKALDEDR
                     VDGEVLYPNGPVSNFAFLQADAEFELACVQAYNDALAEWREVSDRYVPVAIIPYMSEI
                     DVVVAEVERSVKRGHRGVVMLAEPAFTKQGLKRMNDPFWEPLWACCADLGVPMHWHGS
                     SGLVEQLSLPKWNGFTKKEFHTVSTSRLCATPAQLIPNLIFSGILDRHPRLNWACAET
                     GFGWVSYVLEASDHEWETRQLWKEGIKTRPSEVFRRQIYVDFWYEKAGIELREWVGVD
                     NIMWESDYPHIAATYPRSWSFVEDSLVGVPQGEREKMCYGNALRLYGVA"
     gene            complement(5603..>6155)
                     /locus_tag="VE170_17785"
     CDS             complement(5603..>6155)
                     /locus_tag="VE170_17785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17350.1"
                     /translation="APHRYADYLKPEYKNKIAFHIGTNNPLIGMAELNGEDKALAYMR
                     AFSKQGLILHNGYTKISQLLAAGEFSLVGFMQVTKLEKIRERNGPVDWIPLDPSFATV
                     SAVAVSKNAPHSNAARLLVDFYLSEEGQRALREIDKIPLRKGVEADSKRVAELIEQAP
                     HVIKYEGDAGKHIKQFNEIFLGR"
BASE COUNT         1236 a         1912 c         1682 g         1325 t
ORIGIN      
        1 tgttgatacg tcgtcacgaa ggcattctcg cgatttggac attccttttt gggttcttcg
       61 tggccattcc ctacgggacc aaaatatcct tcatgatttt ttccatcggc ttggacacca
      121 actcggactg ctccggcccg acaatcaata gcggaatccc ccgctcctcc aagttgttga
      181 gttcgtcgta cagcgatttg acgcctttac ggccggatat cctgccggtc gatgcgagga
      241 tcttggcgcc ctccgccgat ttcatatagt caaggaatag cgcggcggca tggggatttt
      301 tggtattggt gtagatgccg atggcgccgc tgatatgcgc cggcgtcgga ttgggaaaca
      361 ccagcgcggc cgggcagccg ccttgcttca tgcgcagaag cccatccgga tagatttcca
      421 cggcgacttt gaattcaccg gcgcacagaa gctgcgcctg cagcgtatgg ccgcgccgca
      481 cagtagcgcc atgtttgatc aatccgtcga cgaaggcgcg ggtcttctgc tcgccccaag
      541 cgctcaacca tcccatcaac gcccgctcgg gttccaaatc gatggataga gtgcccttcc
      601 acttggcgtc gaagagatcg tcgtagcctc tcggcgcctc ttctttcttg accagcttgg
      661 tgttgtagcc cagagtcgac acgcccaaac tatcggcgtg ccaaaaacca tccttatccg
      721 tgtactcttt gccgaagttc accgactcgg gcgagtaata gcgcacccag atgcccgagc
      781 gcttgagcgc catcacattt tccgtgccga ccagcaccgc gtcggtatcg agctgcccgg
      841 cgcggtgctc cgtcaaggtg cgaaacacca gtttgttgcc gctgccgcgc cagaactccg
      901 ccttgatgaa cggatagcgc ttcatgaagc cggcggtgta agcaccgagc agcgtggcgg
      961 cggaaatgcc gtagaacttg aaaccgcctt ccttgcgggc gccgtcctca aggcgctttt
     1021 gccgctgcgc cggcggcagt ttgttgatct cgctgtaaag cgcttcgaca gacgcggcat
     1081 gggcgtttga agacagtgaa caaaccaaca gcaatgaagt cactgaatag gccagcaatt
     1141 tcctcggcgc catcttcaat cctccattct cgatcctcga cccggaccta cttcatcgtg
     1201 caatgtggat gataatccat cttgcagcgc gcgccggcct tcttcatcgc cgtttcgaac
     1261 tcggcgtgaa tcaccggatc ttcgtcttcg tactcgatct gatcgccgcc ttctttgacg
     1321 ctcttgtaga cataagcctt gccgccggct ttcggcttgt cgctgcccca accgatggcg
     1381 agaaaaaaca ctggctcggc gctgccgttg aagtgctggt gaaaccacat ttccggcggc
     1441 acgaagacgc tgccgggtcc ccattcgacg cgctccaacg gcttattttc atgccagagg
     1501 atcgaatagc cggtgccgcg gatgatcaaa acgtgaatgc ctggcccatg ccgatgggct
     1561 tttttgtaag cgccgacgcc ccagtgcgag acatgagact tcaccgtctg cttgaccatg
     1621 cgaaacaccg tgctcttggc gccgccgccg cgatcgaaac cgccggcata ggtctcgcac
     1681 acgcgcacgt cggcgacaaa gttggtcgcc cactgctgcg ccggcgcggc ttgcttgaag
     1741 tacccttcct cagagttgta acggtctttg aagacaaagg gattttcgaa aacaaattca
     1801 taattgttga atgtgtcgat aacccgcggc gcgctggtca tggcaatata gcgcgccgaa
     1861 tcgagaccgg acgcgttgtg atgctcatgc caagcattca tcgggatcgc gaaatagctg
     1921 ccctcttgcc attcgaaggt ctctttcttg gtcggatcga tccacaccga agtcgcgccc
     1981 tggcctttga gaatgtagat ggtttcctcg aacaggtggc ggtgcgcctt tagcgatttc
     2041 cccggcggga tttccgcgat ataggaatcg ttgaagccgc cggtgccttc gagattcaga
     2101 aacgccgcca tgcccgcgcc gctggacttc cagggcgcta gctcaacggc gttgacatct
     2161 ttaatcgcca aaccgcgata ggtcggtaag ccttcgtcct ttttccactg ttcgtagggc
     2221 gaaacatacg caactcgctc cttgacttcg tcttccgccg tgaccggtgt gtctgccatg
     2281 ttcgtttcct ccgttttgct ttgactgcgt aattcgcgat taacaatcca cccaactgcg
     2341 accggcaccc tcgctcgatt ccatttccat cgcttcgacg gcgaatgagc cggcaggcta
     2401 gcgcgcgctc actctaccgc caacatcgca tcggcgaggg cgccgcgcaa ggcgcggtcg
     2461 atcagcaagc cgtcccgcgt cgccgccaac acagcctgga ccgccgcttc gctgtcgacg
     2521 taacgtagaa agatcggctc gaagatatct gaatgatcct cgtcagccac cgcgtgaact
     2581 tcggtctgga aatctttctc agcggtcttc acgccgagct cggcgcgctg cttgcgcttc
     2641 cagcgctcga ctaaaccgcc gcctttgacg atcgcgttgt tgttgcgccg ttcgaggata
     2701 tgcgagtaga tcaagccctc taaccacggc ttctcccgcg cgcagtaaag ccacgcgtaa
     2761 tgcgcggctt tggcgccggg cgagagctgc gactcgtcgg ccaattgcaa gtcgttatcg
     2821 gttacatggg cgcggcccat gcgctcgtcg tggatcagct cgtctttctc atgggcccag
     2881 atcgcccgct tgacctcaag cggcgccttg ccggaaacaa acgcccagca gtcgcgccgg
     2941 ttattggcgt agaaggtcat atgattttgg tagacttgca cacggccagg tgtgagcttc
     3001 acggcgaaaa gttttttgaa ttcaggggta tcgaactgcc ggttgaccat ggcatcgatg
     3061 gtcgggcgta tttcaagcca atgagacatg ttaatactcc gctggcgatt caggataaag
     3121 agttcattta cgcaaccgtg atcttgtaca actgcgccgc gttctgccac agaattttct
     3181 gtttgtcggc ctccgctacg ccggcgaaac ttttcgccag cgccgcccgg gtatccggcc
     3241 acgttgaagt ggcttgcggg aattgacttg accataaaat attttccgcg ccgatgtagt
     3301 cgcgcacgcg caggctggcg caatcgtacc agccgaccaa gtaacattgc cgcttgaaca
     3361 actcggaggg catgagatcg tagccgttct gcggcagctg atcgccggtg gcctgaaagt
     3421 cggtatactc cagcaaatag gcgccccagc cgagaccgct tccggccaag acgattttta
     3481 gattggcgaa gcgcattaaa atctttgaga tcaaaagatt gactaacacc gaaaccgagc
     3541 tcgccggccc ggtgatcgcc tgaatcgccg ccgccgtcgc cggcggataa cctgcatacg
     3601 cgggaatttg aatcttcgcc gacgcgccgg catgaaaaca gagcggcacg ccaagttcct
     3661 gacacaccgc ccacaccgga tcgtaaatct cgtcgttgat atgtggcagg tcgcgcagtt
     3721 ccatcggaat cgatggatag accacgccgc gatggccatt ggcgatgcag cggcggattt
     3781 ccgcaaccgt cgacccgatc ggcgccagcg gcacgagaca ttgcggcacg aagcgcggac
     3841 tgacaccggc ccactcttcg agcagccaat cgttgtaagc ctgcacgcag gcgagctcca
     3901 gctcgccatc ttcgatgcgg ccgaagtttt gcccagcgat gcccgcgacc gtcggataga
     3961 gaactgcgtg atcgatgccc gccgcgtcca tcgcccgcag acgttggcga gcatcgtaaa
     4021 ccatcgccgg cacgtcggcc cagcgggttg gatttgccgt ccgtggcgcc atcaccgcgc
     4081 cgcaatcggc gacaccgtcc atggcgatcg gccgaccgtc gatgagccaa cgctcgttgc
     4141 cttgagcatc ttttgccaca tggggaatgc gctcgcccca cttggccgcg gacaagcgct
     4201 tggtccacac gtcgggatgc tcctggacat gctcgtcgac actgatgaat tgttgcgtcg
     4261 ccataaaaaa ataattttga cttgcaaaag tattgcgcca atcaaaccgc ggccgtccag
     4321 gcgaaagtcg gcaacccggt tgccttaaca aggctgttca cggcatgacg gataagaatt
     4381 agcctaggcc acgccataca gccgcagcgc attaccgtag cacatttttt cgcgctcacc
     4441 ttgcggcaca ccgaccagcg agtcctcgac gaaactccac gaccgcggat aagtcgcggc
     4501 gatatgcgga tagtccgact cccacatgat attgtcgacg ccgacccact cgcgcagttc
     4561 gatgccggcc ttctcgtacc agaagtcgac gtagatctgc cggcgaaaca cctcgctggg
     4621 ccgggtcttg atgccttcct tccacagctg ccgcgtctcc cattcgtgat cgctggcttc
     4681 gagcacatag ctcacccagc cgaaaccggt ttcggcgcag gcccagttga ggcgcggatg
     4741 gcggtcgagg ataccggaaa aaattagatt gggaatcagc tgcgccggcg tcgcgcacaa
     4801 gcgcgacgtc gacaccgtgt gaaattcttt tttggtgaaa ccgttccact tgggcaacga
     4861 caattgctcg accagtcccg acgaaccatg ccaatgcatc ggtacgccca ggtcggcgca
     4921 gcaagcccag agcggctccc aaaacggatc gttcatgcgt ttgagccctt gcttggtaaa
     4981 cgccggctcg gccaacatca ccacaccacg gtggccgcgt ttgaccgagc gctcgacctc
     5041 ggcgacgacg acgtctatct cgctcatgta aggaatgatc gccaccggca catagcggtc
     5101 gctgacctcg cgccattccg ccagggcgtc gttatatgcc tgcacgcagg cgagctcgaa
     5161 ttcggcatcg gcctgaagaa acgcgaagtt cgacaccggt ccgttcgggt aaagcacctc
     5221 gccgtcgacc cgatcttcgt ccagcgcttt tagccgcgcg gcgggatcgt agacgatctc
     5281 cggcacttcc tgccagcggc gaggatagta accgcgcgcc acgccgcccg gcatcgccgc
     5341 cggacagttg cacacccact cgctgcgcac cttgccgttg accatccagc ggtcgacccg
     5401 cttgccgtcc tcgtcgactt cgacgatctg cggtatgcgc tcgccccact tggccttgga
     5461 catgcgcttg gtccaggcat cgcggtccaa ctgcgcgtgc gagtcacagc taaacaagcc
     5521 aaatttaact tccaccaatc gctcccctat ccgacgattt gaacgttttg aacggcttga
     5581 acggtttaaa ccgagctatt tttcatcgtc ccaaaaaaat ctcgttaaac tgtttaatat
     5641 gtttccccgc gtcgccctca tacttgatga catggggcgc ctgctcgatg agttcggcga
     5701 cccgcttgga atccgcctcg acacccttgc gcaggggaat cttgtcgatc tcgcgcaacg
     5761 cccgttggcc ttcttcggag agatagaaat cgaccagcag ccgcgcggcg tttgagtggg
     5821 gcgcattctt cgacaccgcc accgcggaga ccgtggcgaa tgacggatcc agggggatcc
     5881 aatccaccgg gccgttgcgc tcacggattt tttccagttt cgtcacctgc atgaacccca
     5941 ccagagagaa ttcaccggcc gccagcaact gagagatctt ggtataacca ttgtgcaaaa
     6001 tgagtccttg tttcgaaaag gcgcgcatgt aggcaagcgc tttgtcttcg ccgttgagct
     6061 ccgccatgcc gatcagcgga ttgttggtgc caatgtgaaa agcgatcttg tttttgtatt
     6121 ccggtttcaa gtagtcggca taacggtgcg gcgcc
//
LOCUS       DASQKS010000686         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5438455, whole genome shotgun sequence.
ACCESSION   DASQKS010000686 DASQKS010000000
VERSION     DASQKS010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5438455"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VE170_17790"
     CDS             <1..272
                     /locus_tag="VE170_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17351.1"
                     /translation="SGRRLATILLAVAQFGRPYVSSPGLPPERAKMLQSAFAKATSDP
                     EFVAEAKRRQLELNPIYGDELNTLAQEVIAQPVEVIERMKQIMGN"
     gene            287..1360
                     /locus_tag="VE170_17795"
     CDS             287..1360
                     /locus_tag="VE170_17795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17352.1"
                     /translation="MKNSIILIAFSCVAIYGISSAHALTTEEIALLSKPDRQKILEEG
                     AKKEGKVVWYTPLIVNQAVRPLKEVFEKKYPFLKVDFHRANSRGLVQKWIAENSAKRY
                     EVDVVGGSEATMLGKKAGLLLRFSSPFSREYPTELKDPQGLWSATNLYFMALAYNTRR
                     IKLNEAPKTYEDLLNPKWKGRMAWHMGSNTGTPLFIGNILSALGEKAGTAYLQNLAKQ
                     GVISTTASARGIVDLVVAGEYDIAINIFNNHAEISKDAGAPIEWQPLEPVPSPIGTTG
                     VAKNAPRPHAAMLFTDFLLSEEGQKVFQRADYLPAHPKVSAKSPELKPGGGRFAKANY
                     FHPETVMEQSDKWVAVQDKIFVK"
     gene            1747..2832
                     /locus_tag="VE170_17800"
     CDS             1747..2832
                     /locus_tag="VE170_17800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17353.1"
                     /translation="MPIIDADTHIDETEATWEYLRDFEQEYKPITQHPSRVDPSKTPA
                     RYWLVDGHRQMRFVRDDANTRTTVETRELLDVPARLRDMDAMGTEVQVLYPTLFLMEA
                     TERPEVSTALRRSYNRWLADRCAESHGRLRWVCLPPLMNMDETIKELQFAKDHGACGI
                     LKKGDMEAGKWPADQYFTPLYEEANRLDLPICVHIGSGTPDFVPAREFSLGSFMRSQL
                     PTVHAFHTIIRHQLPVKYPKLRFGIIEAGSSWIPFIAWELKRRMEKSADGSGTFSQFA
                     RYSLSSDLLKANRMYVTCQVDEDLPYILKVAGEDSLMVGSDYTHRDSSMEYEFPRLLK
                     ERADAGEISKTAVQKILYDNAKTFYGL"
     gene            complement(2931..>3459)
                     /locus_tag="VE170_17805"
     CDS             complement(2931..>3459)
                     /locus_tag="VE170_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17354.1"
                     /translation="NWPSAILVDGAKKEGKLMFYTALIVDQVVRPLKAAFEKEYPFVQ
                     LEFFRANSDRLGQKILTEYQAKRYDVDVVSGSGATTVARQAGDLQKFFSPHTAEYPAE
                     LKDRQGFWGVSNVYFMTLGYNSRMVKPNGRTEGFSSGELSTRASRGAGFAGRSQSRGR
                     QISQGALHNPGSAIR"
BASE COUNT          832 a         1010 c          908 g          709 t
ORIGIN      
        1 agtcgggccg gcgcttggcg acgattctcc tcgccgtcgc gcaattcggc cgcccctacg
       61 tgtcgtcgcc gggattgccg ccggagcggg cgaaaatgtt acagagcgcc tttgccaagg
      121 cgaccagcga tccggagttc gtcgccgaag cgaaacggcg ccaactcgaa ctcaatccga
      181 tctacggcga cgaactgaac acccttgccc aggaagtcat cgctcagccc gtcgaagtta
      241 tcgaacgaat gaaacaaatc atgggcaact gagtaaaaaa agtccgatga aaaactcgat
      301 aatcttgatc gcattctcct gcgtcgccat ctatggcatc agctcggctc acgccttgac
      361 cactgaggag atagccctgc taagcaaacc ggaccgccag aagattctcg aagaaggcgc
      421 caagaaagaa ggtaaagtgg tttggtacac gccgctgatc gtcaaccagg cggtgcgccc
      481 gctcaaagaa gttttcgaga aaaaatatcc attcctcaaa gtcgacttcc atcgcgccaa
      541 ctcgcgcggt ttggtgcaga aatggatcgc ggaaaattcc gcgaagcggt acgaagtcga
      601 cgtagtcggc ggcagtgaag cgaccatgct cggcaagaag gccggtctcc tactgcgctt
      661 ttcctcgccc ttctcgcgcg aatacccgac ggagttgaaa gacccccaag gtctctggag
      721 cgcgaccaat ctttatttca tggcgctcgc ctataacaca cggcgcatca agctcaatga
      781 agcgccgaaa acctatgagg acttattgaa ccccaaatgg aaaggtcgca tggcctggca
      841 catgggctcc aataccggca cgccactgtt tatcggcaac attctttcgg ccctgggaga
      901 aaaagcaggc accgcctatt tgcaaaattt agccaagcaa ggggttatct ccacaacagc
      961 aagcgcccgc ggcatcgtcg atctggtggt cgccggcgag tacgacatcg ccattaatat
     1021 ctttaacaac catgccgaga tcagcaaaga tgccggcgcg ccgatagaat ggcaaccctt
     1081 ggagccggtg ccgtcaccga tagggacaac cggcgtggcg aaaaacgcgc cgcggcccca
     1141 tgcggccatg ctattcaccg actttctcct atccgaggaa ggtcaaaagg tgtttcaacg
     1201 agccgattat ttaccggccc atcccaaggt ttcggcaaaa tctcccgaac tcaaaccagg
     1261 aggcggcaga ttcgccaagg ccaactattt ccatccggaa accgtcatgg aacagagcga
     1321 caaatgggtc gccgttcagg acaagatttt cgtaaagtaa cgattgctat gtcgctacga
     1381 gtacacgaag agcacaaagt tcagagaact aattttccaa aaccttctag tgcttcgtgc
     1441 ccccttcaaa aatacatgtg aggggatgat acctacaacg aagagccgtg cggtctccgt
     1501 catacccgcg aaagcgggta tccaggctaa ttggcccaga acaaacctag attcccgcgt
     1561 gcgcgggaat gacggatcga ggaggggagc aagttctgga tgccaaccat tttgatgctc
     1621 tgtaggcgag cgcaagccat gaacacttcg tgctaacgac cgactttttc tctaagttgc
     1681 acctttgcca accctaactg ttttgtgatt aaaatcgcaa aaagcgaatc tgtaaggagt
     1741 catgccatgc cgatcatcga tgccgacact catatcgacg agaccgaagc cacttgggaa
     1801 tatctccgcg atttcgagca ggagtacaaa ccgatcaccc agcatccgag ccgcgtcgat
     1861 ccgtcaaaga caccggcgcg ctattggctg gtcgacggcc atcgccagat gcgcttcgtg
     1921 cgcgatgacg ccaatacccg caccacggta gaaacccgcg agttgctcga tgtgccggcg
     1981 cggctgcgcg acatggacgc catgggcacg gaagttcaag tgctttatcc gacgttattc
     2041 ttgatggaag ccaccgagcg cccggaagtg tccaccgcgc tgcggcgcag ctataaccgc
     2101 tggctcgccg accgctgcgc ggagtcccat ggccgcttgc gctgggtctg cctgccgccg
     2161 ctgatgaaca tggatgagac catcaaggaa ttacaattcg ccaaagatca tggcgcctgc
     2221 ggcatactca agaaaggcga tatggaagcc ggcaaatggc ctgccgacca gtatttcacc
     2281 ccgctatacg aggaagcaaa tcgtttggac ttgccgatct gtgtgcacat cggctcgggc
     2341 accccggact tcgtgccggc gcgggaattt tccctgggtt ccttcatgcg atcgcaattg
     2401 ccgaccgtcc atgcatttca caccattatc cgtcatcaac tgccggtgaa gtatcccaag
     2461 ctgcgcttcg gcattatcga agccggctcc tcatggattc cgttcatcgc ctgggaactg
     2521 aaacggcgca tggaaaaatc cgccgacggc tccggcacgt ttagccagtt cgcccgctac
     2581 tcgttgtcat ccgacttgct caaagccaac cgcatgtacg tcacctgcca agtcgacgag
     2641 gatttaccgt atattttgaa agtcgccggc gaagatagct tgatggtcgg ctccgattac
     2701 acccaccgcg actcgtcgat ggagtacgaa ttcccgcggt tgctcaaaga gcgcgccgac
     2761 gccggcgaga tctcgaagac cgccgtgcag aagattctct acgacaacgc caaaacgttt
     2821 tacggactgt gagatctgtc agcggacgat ggcagcgggc gtgataaatc gcccaaacgc
     2881 gccatcattt gagaaattga ttataaaagt agtcaaccca ctcgttattc ttatcggatt
     2941 gcagatccgg ggttatgtaa tgcgccctgt gaaatctgcc gcccccggct ttgagatcgg
     3001 cctgcaaagc cggcaccccg ggatgcgcgg gtagatagtt caccgcttga aaaaccttct
     3061 gtccggccgt tgggtttgac catcctgctg ttgtatccca gagtcatgaa atagacattg
     3121 ctcacgcccc aaaatccttg ccggtccttg agctcggccg gatactccgc cgtgtgcggc
     3181 gagaaaaact tttgcaaatc gcccgcctgc cgcgccacgg tcgtggcgcc gctgccgctg
     3241 acgacatcga catcgtatct cttcgcttga tattcggtga ggattttctg gcctaggcgg
     3301 tccgaattgg cgcgaaaaaa ttcgagttgg acaaacggat actctttttc aaaggccgct
     3361 ttcagcggcc ggacgacctg atcgacgatc agcgccgtgt aaaacatcag cttgccttct
     3421 ttcttggccc cgtcgacaag aatggcgctc ggccaattt
//
LOCUS       DASQKS010000687         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4565298, whole genome shotgun sequence.
ACCESSION   DASQKS010000687 DASQKS010000000
VERSION     DASQKS010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4565298"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="VE170_17810"
     CDS             <1..1241
                     /locus_tag="VE170_17810"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17355.1"
                     /translation="PPRWRENCLTSLAVTNIKNDMEEMRNVRAADLKPALSDRRQDFF
                     KLLGSLLLCALIAIPLAVSAQSAKPVWQADWDKTIELAKKEGKVVVSLPASAELRAGI
                     EKLFEKRYGIDVEPVVGRASMLVRKMIDESKAGVRYVDLHMGGSESVVTGMLPGGVVD
                     PLEPYMLLPEVKDPKQWWGGHIWVDNPKKFVYATLAYQSESLWFNSQLMKAEDVRSFD
                     DLLDEKLKDKIGLLDPRTPGSGASMWSYLREVKGEDYLRKLAGQKLALSRDQRVLAEI
                     LAKGKIALVLGLTYYSYAPFIKAGLPVAPLPVPKEGVYVSGGSGHLVVLKNAPHPHAT
                     RLFLNWFMGREGQEVYTKAMHQPTRRLDVDSKWLGEFGVLPAKDHLTVEQYYRRENQS
                     EDKINRLREPAAALARKLLG"
     gene            1251..2363
                     /locus_tag="VE170_17815"
     CDS             1251..2363
                     /locus_tag="VE170_17815"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYJ17356.1"
                     /translation="MMQTRIATLAYLLIAALLLARPGFSQTKNPEWDKVVEAAKKEGR
                     VVVSIPTSAELRKEFESGFQKAFPGIELELNVARGSSNINKIAEEQNAGVRSIDLHIG
                     GTTSIITGLLGPNFLDPVMPSMILPEVRDPKHWWGGHIWADNAKRYVYSFTAYMTETI
                     WYNSTLVKPEEINSWDSLLDPKWKGKIVILDPRSPGSGESNWAFLLKIKGEQFLAKLA
                     AQEMTVGRNLRQLAEAVARGKSAISIGVSYYTYLPFIKAGLPVKSISNVKEGYYAGTG
                     SGNLAVLKNQLHPNASKVFVNWLLSREGQSALTKALGQPTRRFDVDTKWTREFGHPSA
                     KEVLTPEKFEELENGSEEVVFKYRKPAMQLAEKLFR"
     gene            2395..>3056
                     /locus_tag="VE170_17820"
     CDS             2395..>3056
                     /locus_tag="VE170_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17357.1"
                     /translation="MKILSSLSSLVLALLSASSLSAQSPQFRQAEWDKILEAAKKEGK
                     VVASIPPTPELRKLMEIAFSRRYGIATEFVPARGGAVVQRLVSEAKAGVQYFDLHIGG
                     TESVITAMLPENILDAIDPYLILPEVRDPKHWWGGHIYIDNAKRYIYNFVAYQTVSLW
                     SDPNQYKTSEFKSFDDLLSPKLHAKIGISDPRTPGSSSSMWSYMHYVKGEEYLRKLVG
                     QKL"
BASE COUNT          699 a          823 c          897 g          637 t
ORIGIN      
        1 ggccgccgcg ctggcgcgaa aactgtttga ctagtttagc cgtaacgaac atcaaaaatg
       61 acatggaaga aatgcgaaat gtaagggcgg cggatttaaa gcccgctctc tctgataggc
      121 gtcaggattt tttcaaactg ctcggtagtc tattgctgtg cgccctgata gcgattccac
      181 tcgctgtctc cgcgcaatct gcgaaacctg tttggcaagc cgattgggat aagaccattg
      241 agcttgccaa gaaggaaggc aaggtggtgg tgtccttgcc ggccagcgcc gagctgcgcg
      301 ctggtatcga aaagcttttt gagaagcgct acggcatcga tgtcgagccg gttgtcggcc
      361 gggcgtcgat gctggtgcgc aaaatgatcg acgagtccaa ggccggggtg cgctacgtcg
      421 acctgcacat gggcggcagc gagtcggtgg tgaccggcat gttgccgggg ggcgtcgtcg
      481 acccgttaga accctacatg ctgctgcccg aagtcaaaga tcccaagcag tggtggggcg
      541 gccacatctg ggtcgacaac ccgaaaaaat tcgtctacgc caccctggcc tatcagtcgg
      601 aaagcctctg gttcaattcc caactaatga aagccgagga tgtccgcagc ttcgacgacc
      661 tgctcgatga gaaactaaaa gacaaaatcg gcctactcga cccgcgcaca cccgggtcgg
      721 gtgcatccat gtggtcctat ctgcgcgaag tgaagggcga ggattatttg cgcaaactgg
      781 ctggccagaa gctggccctg agtcgcgacc aacgggtgct ggcggagatt ctcgccaagg
      841 gtaaaatcgc gctggtcctc ggactcacct attattccta cgcgcctttc atcaaagccg
      901 gtttgccggt ggcgccgctt cccgtgccca aggaaggtgt ctatgtgagc ggcggcagcg
      961 gccatctggt ggtgttgaag aacgcccctc atccccatgc caccaggctt tttctcaatt
     1021 ggttcatggg ccgggaaggc caggaagttt ataccaaggc gatgcatcaa ccgacgcgac
     1081 gactcgacgt cgactcaaag tggctgggcg agttcggcgt cctgccggcg aaagaccact
     1141 tgaccgtcga gcaatactac cggcgcgaga accagtcgga ggataagatc aatcgcttgc
     1201 gcgagccggc cgcggccctg gcgcgcaaac tgttaggcta gaggtgattc atgatgcaaa
     1261 ctcgaatcgc gacactggca tatcttctga tcgctgcgct tttgcttgcg cgacccggtt
     1321 tctcgcaaac gaaaaacccc gaatgggaca aagttgttga agccgcgaaa aaagaagggc
     1381 gggtcgtcgt gtcgattccg acgagcgccg agttgcgcaa ggaatttgaa agcggcttcc
     1441 aaaaagcttt tcccggcatc gaactcgagc tcaacgttgc gcgcggctcg agtaatatta
     1501 ataaaatcgc cgaagaacaa aacgccggcg tgcgctccat cgatctgcat atcggcggca
     1561 ccacgtcgat aattaccggt ctgctggggc cgaatttctt ggacccggtg atgccgtcga
     1621 tgatccttcc cgaagtcagg gaccctaagc actggtgggg cggccatatc tgggccgaca
     1681 atgccaagcg ttacgtttat agctttaccg cctatatgac cgagacgatc tggtacaact
     1741 cgaccttggt caagccggaa gaaatcaact cgtgggatag tttgttagat ccgaagtgga
     1801 agggaaagat cgtgatcctg gacccgcggt cgccgggctc gggagaatcg aattgggctt
     1861 ttctattgaa gatcaaaggc gaacagtttc tcgccaagct cgcggcgcag gagatgacgg
     1921 tcggacgtaa cctgcgccag ctcgccgagg cggtggcgcg tggcaagtcc gcgatctcca
     1981 tcggcgtctc ctattacacc tatctgccgt tcatcaaagc tgggctgccg gtcaagtcga
     2041 tttcgaacgt caaagagggc tattacgccg gcacgggtag cggcaatctg gcggtgttga
     2101 aaaatcaact tcatcccaat gcatccaagg tattcgtcaa ctggctgctg tccagagaag
     2161 gccagagcgc gctcaccaag gcattgggac agccgacgcg gcgtttcgac gtcgatacca
     2221 agtggaccag agagtttggt cacccgtcgg ccaaggaagt tcttactccg gaaaagttcg
     2281 aagagttgga gaacggctcc gaagaggtcg tcttcaagta tcgcaaaccg gctatgcaat
     2341 tggcggagaa attgttcagg tgagtctagg aactcttcgt tggtgagtgg aaacatgaag
     2401 atactatcat cattgtcatc gttggttttg gcgcttttga gcgcctcatc gctatcggcg
     2461 cagagcccgc agtttcgcca agccgagtgg gacaagattc ttgaagcggc caagaaggaa
     2521 ggcaaggtgg tcgcctcgat tccaccgacg cccgagttac gcaagctcat ggagatcgcg
     2581 tttagccggc gttacggcat cgccactgag ttcgtgccgg cgcgcggcgg cgcggtggtg
     2641 caacggctcg tcagcgaagc caaggccggc gtgcaatatt tcgatctgca tatcggcggc
     2701 acggagtcgg tgatcaccgc catgttgccg gagaatattc tcgacgccat cgatccctat
     2761 ctgattttac cggaagtgcg cgaccccaaa cattggtggg gcggccatat ctatatcgac
     2821 aatgccaagc gttacatcta caacttcgtc gcctatcaaa cggtcagtct gtggagtgat
     2881 ccgaatcagt acaaaaccag tgaattcaag tcgttcgacg acctcttaag cccgaaattg
     2941 cacgccaaga tcggcatcag cgatccgcgc acgccgggtt ccagttcgtc gatgtggtcg
     3001 tatatgcatt acgtcaaggg cgaggagtat ctgcgcaaac tcgtcggcca aaaact
//
LOCUS       DASQKS010000688         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5440797, whole genome shotgun sequence.
ACCESSION   DASQKS010000688 DASQKS010000000
VERSION     DASQKS010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5440797"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..628
                     /locus_tag="VE170_17825"
     CDS             314..628
                     /locus_tag="VE170_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ17358.1"
                     /translation="MGTKLYVGGLPYSVTEGKLQDIFSAHGTVESANVISDKFTGQSR
                     GFGFVEMSTSGEAQKAIETLNGTQLDGRTLTVNEAKPMARRDGGGGGGGGGSRDGGRN
                     RW"
     gene            867..1235
                     /locus_tag="VE170_17830"
     CDS             867..1235
                     /locus_tag="VE170_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17359.1"
                     /translation="MAENESVKQSANLLRTIIRSIDKKLEYSLIDPTQEGRFSLRLTT
                     RGREGSVSIRTDDLRRAGVDDVRRNGIRQKIKSARDHLLSNYVADVMGRRVARMLKQS
                     SAGKDDAKSSFFYRRPQGRR"
     gene            1568..3337
                     /locus_tag="VE170_17835"
     CDS             1568..3337
                     /locus_tag="VE170_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HYJ17360.1"
                     /translation="MIKINQLSKAYFTRELFADVTFQMNAGDRLGLVGRNGHGKSTLF
                     KLILGEEEIDSGDITIPRNYRIGHLEQHLHFTQRTILEEAALGLPEEEVHSIYKAEAI
                     LFGLGFSPADLEKAPGEFSGGFQIRINLAKLLLSDPNLLLLDEPTNYLDITSVRWIAK
                     FLTNFKGELILISHDRAFMDSVTTHTAVIHRQKIRKFEGGTAKAYAQIVLEDEIHEKT
                     RANEDRKRAHAEAFINRFRAQASKAKMVQSRIKMLERLPDLGELTEIESLDFEFRYAP
                     FAAKTLLEARDISFGYSADNLLFKDISLCVNARDRIGVIGNNGKGKSTLLNVLSGGLT
                     PLTGEVKLHPDLKSGFFGQTNIQRLDPKLTIEQEITNANPALTRTQVRNICGTMMFGG
                     DLALKKVTVLSGGERSRTLLAKILAHPSNLLLLDEPNNHLDMESIDALIESLQDFPGA
                     LLIVTHNEGILRALATKLIVFHRGKADVFDNTTYDEFLEKIGWEDEEPGKGKSNGVPK
                     KPTTRVNHDEKKEQAKAERKEKARIEKLEAQIIKNEAALKKYNEQLVLEANRNNLGQM
                     RELSVKISQVKREIDDLYGQYAE"
     gene            3925..4242
                     /locus_tag="VE170_17840"
     CDS             3925..4242
                     /locus_tag="VE170_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17361.1"
                     /translation="MPMTDAPVKIPDWAHLYQDSLTTEILRSERRRVTILAVLFAVAA
                     IFSSSISLVPGLLDAEFRARFQSRWPWIISLYAAVVVYELAVRAAIGWLIEHGRQPHV
                     TLR"
     gene            4243..4563
                     /locus_tag="VE170_17845"
     CDS             4243..4563
                     /locus_tag="VE170_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17362.1"
                     /translation="MNAFFETSIPTLMLSIAVPLMGPRDALGSPAFLLYFFFILLSIL
                     RLEMRLCLFTGLVAAVEHFIFAYVAIATSVPEAEAALWRVPAYHFYRSLVLFCGGTRH
                     RAAR"
     gene            complement(4560..>4796)
                     /locus_tag="VE170_17850"
     CDS             complement(4560..>4796)
                     /locus_tag="VE170_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17363.1"
                     /translation="KRTAYSNRPLPLPAGRQPEVAEVTNIQEHYADVAQFAGQFDRLS
                     KQLLGDLRRDVLTEDANRAIAPCVTNLRLGAGFV"
BASE COUNT         1145 a         1320 c         1251 g         1080 t
ORIGIN      
        1 aatcaccagc gtaatagttt cttcgatgac aaatatcaag tgcgcggcct gacgctggtg
       61 atgcaatggc tcggctgttg cttgacacct ccggtctttc cgcgtactgt gagtttccta
      121 cttcagtaaa gggtcttctg aatgaggacg atgcaaacgt ccaaggcccc taatccgtct
      181 atcttcttgc gaacgatcac taaaggatat tccgaccaga gcgtggtttc agcagtcgta
      241 agcccatgtt ctgccctttg gcagtctaat tctcagggtc aaagacagca attttctctc
      301 ttaggaggat aacatgggta ctaaattata cgtcggcggt ctgccttatt cggttaccga
      361 aggcaaactg caggacattt tctcagcgca tggcactgtc gagtcggcaa atgtcatttc
      421 cgataagttc accggacagt cgcgcgggtt tggctttgtc gaaatgagta ctagtggcga
      481 agcacaaaag gctattgaaa cgcttaacgg cacacaactc gacggccgta cgctcaccgt
      541 caatgaagcc aagccaatgg ctcggcgcga tggcggtggt ggaggcggcg gcggcggttc
      601 ccgcgatggc ggcagaaatc gctggtagtc gaactgataa ttagctaaat gctagtgggg
      661 gcaactggtc gggttgcccc ctttttttct cacaaaaccc gtgcgaatcg ctgagtcata
      721 gcggcagcgt tttttaaact gccgctcccg gcgcagcctt tcgatcgaaa gcgttgccga
      781 cttgttcacg ggtaattcca ttgggccacg aggacatgcg gtaacctcgg cgccatattt
      841 atctcgccgc aaaccacgga ggtcccatgg ccgaaaacga atccgttaaa caaagcgcaa
      901 atttgctgag aaccatcatt cgcagtatcg ataagaaact ggagtatagc ttaatcgatc
      961 cgacccaaga aggccgattt tcccttcgac tgacaacccg tgggcgcgaa ggatcggtat
     1021 ccatacgcac cgatgattta cgtcgagccg gcgtcgatga tgtccgccgg aatggtatcc
     1081 gacaaaagat caaaagtgcc cgggatcatc tcttgtcgaa ctatgtcgcc gacgtcatgg
     1141 gccggagagt cgccaggatg ctgaaacagt ccagcgccgg caaggacgac gccaaatcct
     1201 cgttcttcta cagacgcccg cagggaagac gataaccgct gctcgaattc tgagatttat
     1261 tttacgatga caagcgaacc ggcgagatgg gcctcggcct aatggtccat cttgccgccc
     1321 cgttcgttgc gcttcattag caattgcggc ttgcacattt tcaccggtgg cgagtcttct
     1381 gtcaaaaaac gatttgttag aaacccttgc ctttgcgcaa tatcaaacgg aacgaaatct
     1441 tccattccat ccgcaggcta atggcgcctg gtcaactttt cgagtcgttc cgcgtttatg
     1501 gcaagcgctg aaaaatcccc agccagtcgc ttgattaccg taaatttctc ctctaaaaat
     1561 tttcatcgtg attaagatca accaactatc taaggcgtat ttcactcggg agcttttcgc
     1621 tgacgttact tttcagatga atgctggcga ccggttgggt ctggtcggcc gcaacggcca
     1681 tggcaagtcg acgctattta aacttatttt gggcgaggaa gagatcgact ccggcgacat
     1741 caccatcccg cgcaactatc ggattggcca tctcgaacag cacctacact ttactcaaag
     1801 gacgattctc gaggaagccg cattaggctt gccggaagaa gaagttcatt cgatctacaa
     1861 ggccgaagcg atcctatttg gactcgggtt ttccccggcc gatctggaaa aagcgccagg
     1921 cgagttttcc ggcggatttc agatacgcat caacctcgcc aagcttttgc tttccgatcc
     1981 caacttgctg ttgctcgacg aaccgaccaa ctacctggac atcacctcgg tgcgttggat
     2041 cgcgaaattt ctgaccaatt tcaagggcga gctaattttg atctcccatg atcgcgcctt
     2101 catggacagc gtcacgaccc acacggcggt gatccaccgg cagaagattc gcaaattcga
     2161 aggcggcacg gccaaggctt acgcgcagat cgtcctcgaa gacgaaatcc atgagaagac
     2221 tcgcgccaac gaggaccgca aacgggccca tgcagaggcc ttcatcaacc gatttcgcgc
     2281 gcaggcttca aaggccaaga tggtccagtc acgcatcaag atgctcgagc ggctgccgga
     2341 cctcggcgaa ctaacggaga tcgagtcgtt ggattttgaa tttcgttacg cgccgtttgc
     2401 cgcgaaaacg cttctcgagg cgcgcgatat ctcgtttggc tacagcgccg acaatctgtt
     2461 gtttaaggac attagcctgt gcgtcaacgc ccgcgaccgc atcggcgtaa tcggcaacaa
     2521 cggcaaaggg aaatcgacgt tgctaaatgt tctatccggc ggcctcacac cgctgacagg
     2581 cgaagtcaaa ttgcaccccg acctgaaatc cggcttcttt ggccagacca acatacaacg
     2641 gctcgatccc aagctgacta tcgaacagga aatcaccaac gccaacccgg cattgacgcg
     2701 cacgcaggtg cgcaacattt gcggcactat gatgtttggc ggcgatctgg cgttaaagaa
     2761 ggtgactgtg ctctctggag gcgagaggag ccgtacgctc ttggccaaaa ttctcgctca
     2821 tccgtcgaat ctgcttttgc tcgacgagcc caacaaccat ctcgacatgg aatccatcga
     2881 cgctttgatc gagagcctgc aagattttcc cggtgcattg ctgatagtca cccacaacga
     2941 aggcatcctg cgcgccctgg cgacgaaact gatcgtcttt caccgcggca aagccgatgt
     3001 cttcgataat accacctacg acgagtttct cgaaaagatc ggttgggagg acgaggagcc
     3061 gggcaagggc aagtccaacg gcgtcccaaa gaaaccgacg acgcgagtca atcacgacga
     3121 gaaaaaagaa caggcaaagg ccgagcgtaa ggaaaaagcc cgcatcgaga agctcgaagc
     3181 gcagatcatc aagaacgaag ccgcattaaa gaaatacaac gagcaactcg tgctcgaagc
     3241 caatcgcaat aatcttggcc agatgcgcga actgtcggtg aagattagtc aggtgaaacg
     3301 ggaaatcgat gatttgtatg gccaatatgc agagtagagg agaagcttaa gtggccttat
     3361 tcacgggata accgagccat cctgctagaa tcgccaactc gaattgggtt ggccgatggg
     3421 acggcgcata caaaggcttt gcggcacaat aagcgaggca aacaccgttg agtcataccc
     3481 agccatagcc aaagccgagt acttagagac cgcgtcatac ccatccactc gcccctatcg
     3541 cctgcaggcc aaggtctttg caatcgacta gtttgaaccc tccgcgaaac cacaagaagc
     3601 atcagcagga tcgcagtggg cgcgtgaacg tggcaacgca cagcgtccgc tgcactgaaa
     3661 acgcgcttga aacgcgccgg tgacgcccag cgcgaagacg ataatttaat gcaggatctc
     3721 tctgtcgagg ctgataaaca tcaacggtgc caacctcctt cattcgagga gatcgatttc
     3781 ttattggcgg gtgccggaag gtgaagtaag ggaatggtga tttataatcc gaagatcacc
     3841 gcgctgcttg aagaattgaa ccatcaaaga gaatggtgca acgtctctct cacgacccaa
     3901 aaaagtaact gatcccgttt gtccatgcca atgaccgacg cgccggttaa gatccccgac
     3961 tgggcccact tatatcagga ctcgctgacc acggaaattc tccgcagcga gcggcgtcga
     4021 gtgacgattt tagcggtgct cttcgcggtc gctgccattt tctccagttc aatttcatta
     4081 gtacctggtt tgctcgatgc tgagtttcgc gctcgcttcc aatctcgatg gccttggata
     4141 atctcgttat acgctgccgt ggtcgtctac gaattggcgg tgcgtgccgc cattggttgg
     4201 ctgatcgagc atgggcgcca gcctcatgtc acgctgcgct aagtgaacgc attcttcgag
     4261 acctccatac caaccttgat gttgtccata gcggtaccct tgatgggccc gcgcgacgca
     4321 ctgggctccc cggcgtttct gctctacttc ttttttattc tgctgtcgat cctgcggctg
     4381 gaaatgcggc tctgcctctt taccggcttg gttgccgcgg tagagcattt cattttcgcc
     4441 tacgttgcaa tcgccacgtc ggtacccgag gcggaggcgg cgctgtggcg cgtacccgct
     4501 tatcattttt accgatcgct tgtgctgttt tgtggcggga ctcgtcaccg ggctgctcgc
     4561 taaacaaatc cggcgccaag tcgaaggttc gttacacacg gcgcgatcgc gcggttcgca
     4621 tcttcggtca acacgtctcg ccgcagatcg ccgagaagct gcttagacaa tcggtcgaac
     4681 tggccggcga attgcgcaac gtctgcgtaa tgttcttgga tattcgtaac ttcagcaact
     4741 tcgggctgac gccccgccgg cagcggtaat ggcctatttg aatacgctgt tcgctt
//
LOCUS       DASQKS010000689         7428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_1664036, whole genome shotgun sequence.
ACCESSION   DASQKS010000689 DASQKS010000000
VERSION     DASQKS010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664036"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VE170_17855"
     CDS             complement(<1..562)
                     /locus_tag="VE170_17855"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYJ17364.1"
                     /translation="MDSPIFNGAQLRSDGPRNGLLKLAIDGRLDADSTGRVWRESTRL
                     VAAARAATVDLDASAMDYCDGSGIALLIQLRRLQQKAGGNLEIHGLRPEFQELLDDWS
                     QGSSDTTQSKAKVRVQLVEEIGRGAVQVWEDIHELISFVGQLGLAMVKAARHPSAIRW
                     RDTLLIAEEVGVNALPIVALLSFLVGV"
     gene            complement(614..1915)
                     /locus_tag="VE170_17860"
     CDS             complement(614..1915)
                     /locus_tag="VE170_17860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ17365.1"
                     /translation="MNFPSTFATAKSKIFYGWYIVGVGFLSHVACAFHMSSTLSVFLK
                     PLTEDLGVSRGLFSLLRSGEILIGAAMAPLVGPMIDRFGGRWLMAVGALSAGLGFVLM
                     SQVGAFWQFLLLRWIFVAIGGVFMCHMIVSVTISRWFVRKRGRAIAIASMGQGFAKVC
                     IPVLTAALFTWVGWRLTWSIFGLITLVLVVIPALIFMRRSPEELGLQPDGMDAPPGTF
                     DVNQSNDDRKASLSTEEVIWTWREVIRTKAFWLICFIYGLANVGIAGLNLHVFAYVTD
                     IGFSPLVAATVLSIIASTQLGSTMVWGVISERVDIRHSSLIMFLVQSAGLALVLVTSH
                     ITPVYLGFFLYGIGLGGGWVLQELIWAANFGRISLGMVRGLGILVTHAFGAAGAPFFG
                     FVHDVTGSYRSSFLAFVVALMIAAYLSLAIRAPQKNSAPAS"
     gene            2058..2408
                     /locus_tag="VE170_17865"
     CDS             2058..2408
                     /locus_tag="VE170_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ17366.1"
                     /translation="MAKRGLSHIALKVRDLKKTEAFYVGVLGLKVAFRHPPGMIFLTT
                     PGSGDLLNFVKTAERPRANQGLEHLGFRVTSTELKKLEAKIAQAGVKIDGRRGHTAFY
                     ISDPNGYQLEYYCD"
     gene            complement(2466..3521)
                     /locus_tag="VE170_17870"
     CDS             complement(2466..3521)
                     /locus_tag="VE170_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HYJ17367.1"
                     /translation="MNTPPTLRGRGSATNPKNRFESIERVPEPPENSDEISAPHTQFY
                     PDSSKSLIAFNDSPDVGFDASINPYRGCEHGCIYCYARPSHEYLGFSAGLDFETKIMV
                     KEDAPELLRKELSSPKWKPQLVALSGNTDCYQPIEKQKRLTRRCLEVLLEFRNPAVII
                     TKNHLVTRDIDILSELAKIDCIGVTISLTTLDGKLASALEPRGSTPARRLAAIRKLAA
                     AGVPVGYLQAPMIPGLTDSEAPAIAIAAAQAGASFSGYVALRLPFAVKDLFEQWLTQH
                     YPDKKDKILHRVREIRGGKLNDPNFKSRMRGEGIYAEQMAALFQLARMKSRINERWPK
                     LTTEHFRRPEKNQLTLF"
     gene            complement(3536..3862)
                     /locus_tag="VE170_17875"
     CDS             complement(3536..3862)
                     /locus_tag="VE170_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17368.1"
                     /translation="MEANALSTAKQNIRQLAKQKAELAAATPNRKKTRLLQRKIRLLK
                     RVTRVLATEKKAVVAKAAAEAAAKEAAEKAAAAKAAADAKAAAAKAAAEADKAAESAS
                     ETEVSA"
     gene            3922..4587
                     /gene="nth"
                     /locus_tag="VE170_17880"
     CDS             3922..4587
                     /gene="nth"
                     /locus_tag="VE170_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYJ17369.1"
                     /translation="MTAKKSIPSDTDPRDRLKTILPRLEKSHPDAKLDLDFTNPLELL
                     ISLVLAAQARDDLVCKITPALFKANRSAADYAKLPLADLETQIGKINFYRNKARSIHN
                     CCVQLVERFGGKVPDNLADLVSLPGVGRKTANVVLGNGFGQQTIGVDTHVKRLSQRLG
                     FTPKTDPDKIEADLMAIVPEQHRVRFCHLMQYHGRRVCLAKKPRCPECIINDLCPFPK
                     KTV"
     gene            4729..5490
                     /locus_tag="VE170_17885"
     CDS             4729..5490
                     /locus_tag="VE170_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17370.1"
                     /translation="MSDILQPPSSSYVMREARGLIELPRLLLRFPALARLPRGNGEPI
                     LILPGYGAGDGSTMILKGYLRLLGYRARGWGLGRNRGAASESLPRLLRRLGNLSRRSK
                     QKVAIIGWSFGGYLARELARERPDLVRLVITLGTPVVGGPKYTAAAKSYRKRGIDIEA
                     IAAEVEQRSRLADLEIPVVAIYSRDDSVVAWEACIDRLSPNIEHVEIKSTHLGFGFSA
                     DVYQIIARRLAQFESQGTPLTTEPRRRRRLPKLPR"
     gene            5535..6020
                     /locus_tag="VE170_17890"
     CDS             5535..6020
                     /locus_tag="VE170_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HYJ17371.1"
                     /translation="METSILYWLGAALLVVVGLIGLVLPAIPGALLILTGLILGAWAE
                     GFAHVGLWTIAILTVMAGLTYAVDFWATMFGVKKFSASKRAVIGALIGVVAGLFLGVP
                     GVIFGPFIGAVIGELSARRNLQQATRAGIGAIIGIVLGAALKIALALAMIGIFVATRF
                     L"
     gene            complement(6122..6514)
                     /gene="gloA"
                     /locus_tag="VE170_17895"
     CDS             complement(6122..6514)
                     /gene="gloA"
                     /locus_tag="VE170_17895"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HYJ17372.1"
                     /translation="MKIVHTMIRVNNLEESLKFYCDGLGMKLIRKKDYESGRFTNVFV
                     GYGSEDTDAVIELTYNWDTHSYDIGNGFGHIALGVPDVYKTCDELRAKGVKIIRQPGP
                     MKHGSGTHAIAFLEDPNGYKIELTTRAE"
     gene            complement(6826..>7428)
                     /locus_tag="VE170_17900"
     CDS             complement(6826..>7428)
                     /locus_tag="VE170_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYJ17373.1"
                     /translation="QMPEMDGFEASRQIQILKQQAALQSSVIAITAHARKEDRDKCLA
                     WGFDDYVSKPIMPRELKAAIERTMIKSGSNQTSPAPVSDGNDGDVFNFTGALTNVEGD
                     RVLFGKISRMFLVQYPVLLAEIRRALSSEESSALAGAVHTLGSSAGQVGANRVLRLAR
                     NIEECGERSDLTKVPATLAALEAEIESVKSALVQQGWASA"
BASE COUNT         1661 a         2202 c         1990 g         1575 t
ORIGIN      
        1 tgacgccaac gagaaaactc agcaatgcga cgattggtag agcattgacg ccgacctctt
       61 cagcgatcag tagcgtatcg cgccagcgaa ttgcgctcgg atggcgcgcc gccttgacca
      121 tagccaggcc caattgaccg acgaatgaaa tcaattcatg aatatcttcc caaacctgca
      181 ccgccccacg gcctatctcc tcgaccagct gcacgcgaac tttggctttg ctttgggtag
      241 tgtcgctgct gccttgggac caatcgtcga gcagctcttg aaactccggg cgcaggccgt
      301 ggatctccag gttgccgccg gccttttgct gcagccggcg caattgaatc agcagagcga
      361 tgcccgaacc gtcacaataa tccatcgcgg aagcgtcgag atccaccgtc gccgcccgcg
      421 ccgccgctac cagccgagtc gattcgcgcc acacgcgtcc ggtgctgtcg gcgtcgagtc
      481 gcccgtcgat ggcgagtttg agcaggccgt tgcgcgggcc gtcgctgcgc agttgggcgc
      541 cattgaagat gggtgagtcc atttgaagtt aattgcggcc ggtgtcttta ttatgcgcgg
      601 tccatcggca tggtcaactc gccggggccg agtttttttg cggtgcgcgt atcgcgaggc
      661 ttaggtacgc cgcgatcatc aaggcgacga caaaagctag aaacgacgac cgataactgc
      721 ctgtaacgtc gtggacaaaa ccgaagaacg gcgctcccgc ggcgccgaac gcgtgggtta
      781 cgagaatacc cagaccgcgc accatgccga gggagatgcg gccgaagttg gccgcccaaa
      841 tcaactcttg cagcacccag ccaccgccca aaccgatgcc gtaaagaaaa aagcccaagt
      901 agaccggcgt gatatggctg gtgacgagca cgagcgccaa gcccgccgac tgaaccaaaa
      961 acatgattaa acttgaatgg cggatatcca cgcgttcgct gatcacgccc cacaccatgg
     1021 tcgacccaag ctgggttgag gcgatgatgc taagcaccgt ggccgccact agcggcgaaa
     1081 agccgatgtc ggtgacgtac gcgaacacat gcagattcaa accggcgata ccgacgttgg
     1141 ccagaccgta gatgaaacag ataagccaaa acgctttggt gcggataacc tcgcgccagg
     1201 tccaaatcac ttcctccgtc gacaagcttg ctttcctgtc gtcgttcgat tgattgacgt
     1261 cgaaagtacc tggcggcgcg tccatgccat ccggctgcag gcccagctct tccgggctgc
     1321 ggcgcataaa gattaaagcg ggaatcacca ccaacaccaa ggtaatcaga ccgaagattg
     1381 accaagtcag ccgccagccg acccaggtga acagcgccgc agtcaaaacc ggaatacaaa
     1441 ctttggcgaa gccctggccc atgctggcaa tggcgatggc acgcccccgt ttgcgcacga
     1501 accagcgtga tattgtcacg ctgacgatca tgtggcacat gaacacgccg ccgatggcga
     1561 cgaaaatcca gcgcagcaaa agaaattgcc agaacgcgcc gacctggctc atcagcacga
     1621 aacctaatcc agccgaaagc gcgccaacgg ccatcaacca gcgaccgccg aaacgatcga
     1681 tcatcggtcc caccagcggc gccatcgccg cgccaatgag gatttctccc gagcgcaaga
     1741 gcgaaaacaa accgcgcgat acccccaagt cctcggttag cggtttcaag aacacgctca
     1801 gcgtactcga catgtgaaag gcgcaagcca catgggacag gaagccgaca ccgacgatat
     1861 accagccgta gaagatcttg gatttggcag tggcaaatgt tgacggaaaa ttcaagtcgc
     1921 agccacccga ggaagagcat tcaaattatc aaggatcgcc gcgcttgtaa aagcgccggc
     1981 ggttgcacca gcaagaaccg atgcgatata aaaactcatc tgacagcgat catgaaccac
     2041 gtcacaggag ccgagccatg gcaaaaagag gcctgagcca cattgcgctt aaagtgcgcg
     2101 atttgaaaaa gaccgaggcc ttctacgtcg gcgtcttagg gctaaaagta gcgtttcgcc
     2161 acccgcccgg catgattttc ctgaccacgc ccggcagcgg cgatctgcta aacttcgtca
     2221 aaacggccga acgcccgcgc gccaaccaag gcctggagca tttgggcttc agagtcacca
     2281 gtacggagct gaaaaagctt gaagcaaaaa tcgcccaggc cggggtgaaa atcgacggcc
     2341 gccgcggcca caccgccttc tacatcagcg atcccaacgg ctatcagctg gaatattatt
     2401 gtgactaact ggcgctggat ccacacagtt tattttatcc ccacccgatc gccagcctgt
     2461 cgcgttcaaa acaaagtcaa ctgattcttc tccggccggc gaaagtgttc cgtcgtcagc
     2521 ttcggccagc gttcgttgat ccgcgatttc atgcgcgcta gttgaaacag cgccgccatc
     2581 tgctcggcgt aaatcccttc gccgcgcata cgcgatttga agttgggatc gttgagcttg
     2641 ccaccgcgga tctcgcgcac gcggtggaga atcttatcct tcttgtccgg ataatgctgg
     2701 gtcagccact gctcgaataa gtctttcacg gcgaacggca atcgcaatgc gacataaccg
     2761 gagaaggatg cgcccgcctg cgctgcggcg atggcgatgg ccggcgcttc ggagtcagtc
     2821 aggccgggaa tcatcggcgc ttgcagatat cccaccggca caccggcggc cgctagtttt
     2881 cgaatcgccg ccagacggcg cgccggagta gacccgcgcg gttccagcgc cgacgcgagt
     2941 ttcccgtcta gggtcgttaa agagatcgtc acgccgatgc agtcaatctt ggccaactct
     3001 gaaagaatgt cgatgtcacg ggtgactaaa tggtttttgg tgatgatgac cgcgggattg
     3061 cgaaactcca aaagtacttc caaacagcgc cgcgtcagcc gcttttgctt ctcaataggc
     3121 tggtagcaat cggtattacc gctcagcgcc accaactgcg gcttccattt tggactcgac
     3181 agctctttgc gcaagagttc cggcgcgtct tccttgacca tgattttggt ctcgaagtcg
     3241 aggccggcgg agaagccgag atactcatgc gacgggcgcg cgtagcaata aatgcaacca
     3301 tgctcgcagc cccgataagg attgatgctg gcatcgaagc cgacatcggg gctgtcgttg
     3361 aaggcgatca gggatttcga actgtcggga taaaactggg tgtgaggagc agaaatctcg
     3421 tcgctattct cgggcggctc gggtacccgc tcgatcgact caaaacggtt tttcggattg
     3481 gtcgccgaac cgcggccgcg cagagtaggc ggcgtattca tgacttttca aaaaactaag
     3541 cgctgacttc ggtctctgac gcgctttcgg cagccttatc ggcctcggcg gccgctttgg
     3601 cggcagcagc tttagcatcg gcggcggctt tggcggcagc agccttttcc gcagcctctt
     3661 tggcggcggc ttcggcggca gccttagcca ccacagcctt cttttccgtg gcaagcactc
     3721 gggtaacgcg cttgagcagg cggatttttc tctgtaacag gcgcgttttc ttacgattcg
     3781 gagtagcagc ggcgagttcg gccttttgtt ttgctaattg gcggatgttt tgtttggcag
     3841 tggataatgc gttggcttcc atcgagatat tcctcccttt atgagttaca gtttggtatc
     3901 atatttttcg gtgaaactaa aatgaccgcg aaaaagagca taccgtccga taccgatccg
     3961 agggatcgac ttaaaacgat cctgcccagg ctggaaaaat cacatcccga tgccaagctc
     4021 gatctcgatt tcaccaatcc gttggaattg ctcatctccc tcgtcctcgc cgcgcaagcg
     4081 cgcgacgact tggtctgtaa aatcacaccg gcactattca aagcaaaccg gagcgctgct
     4141 gattacgcga agctgccgct cgccgatttg gaaacgcaaa tcggaaaaat taatttctac
     4201 cgcaacaaag ccagatcgat tcacaactgt tgcgtgcaac tggttgagcg cttcggcggt
     4261 aaagttcccg acaatctagc agacctcgtc agtctccccg gagtcggccg taagacagct
     4321 aatgtcgtcc ttggcaacgg ttttggccag caaaccatcg gcgtcgacac ccatgtcaag
     4381 cgcctatcgc agcggcttgg cttcaccccc aaaactgacc cggacaagat cgaagccgac
     4441 ttgatggcaa tagtcccgga acaacatcgg gtgcgctttt gccacctgat gcagtaccac
     4501 gggcgccgcg tctgtttggc caagaaacct aggtgcccgg agtgcatcat caatgatctc
     4561 tgcccatttc cgaagaagac cgtttagcca ttcagagtcc tagaaccaat gaacctactt
     4621 ccaatgttat ggctcattgc acaagcagtg atcattcacc gcggcgattg ttaggccagg
     4681 gatttttgtg agcattgaac ctctcgtatt gagccaccac cgaaacccat gagtgatatc
     4741 ctccaaccgc cgtcatcgtc ctacgtaatg cgcgaagcgc gcgggctgat cgagctgcca
     4801 cggcttttgc taagattccc cgcccttgcg cgcttgcctc gcggcaatgg cgaaccgatt
     4861 ctaatccttc ctggctatgg cgccggcgac ggttcgacga tgattctcaa gggttatttg
     4921 cgtctactcg gctatcgcgc gcggggatgg ggactgggcc gcaatcgcgg cgctgccagc
     4981 gagtcgctgc ctcgactttt gcgacggttg ggaaatttat cccgacggag caagcaaaag
     5041 gtcgccatca tcggctggag cttcggcggt tacttggccc gcgagctggc gcgcgagcgt
     5101 cccgatctcg ttcgtctagt gattaccctg ggtacgcctg tcgtcggcgg ccccaagtat
     5161 acggctgcgg cgaaatccta tcgcaagcgt ggcatcgata tcgaagccat cgccgccgaa
     5221 gttgagcagc gcagtagatt ggccgacctg gagattcccg tcgttgcgat ttattcgcgc
     5281 gacgactcgg tcgtggcctg ggaggcctgc atcgatcgac tatctcccaa catcgaacat
     5341 gtcgaaatta aatccactca cctcggtttc ggtttctccg ccgatgttta ccagatcatc
     5401 gcccggcggc tggcacagtt cgaaagccaa ggcacacccc tgactacgga accacggcgg
     5461 cgccgaaggc tgcccaaact cccgcgctaa aacgaacctc agccgccagg gtcaacgaat
     5521 gcgaacgtga acccatggaa acaagcatct tgtactggct tggcgcagcg ctgctggtgg
     5581 ttgtcggctt aattggactc gtcctgcccg ccattcctgg cgcgctgcta atcctgacgg
     5641 gtctcatcct gggcgcatgg gccgaaggat ttgctcacgt cggcttatgg acgatcgcga
     5701 ttcttacggt tatggcggga ttgacctacg ccgtcgattt ctgggccacc atgttcggcg
     5761 taaaaaaatt cagcgcctct aagcgcgccg tcataggcgc tcttattgga gtcgtcgccg
     5821 gcctgtttct cggcgtgccc ggcgtcatct ttggaccgtt catcggggca gtgatcggcg
     5881 agctgtcagc ccggagaaat ttgcaacagg cgacccgtgc cggcatcggc gccatcatcg
     5941 gcatagttct cggcgccgcg ctcaaaatcg ccttggcttt agcgatgatc ggaattttcg
     6001 tggcgacaag atttttgtag tttcaggcga aaaccgtacc ggcgataatc ctcgccgctc
     6061 taacaagccg cgctagcctg aattgcgatc tatgggcttt ctgtcgagcc cgagaggacc
     6121 cctattccgc ccgtgtcgtc aactcaatct tatagccgtt aggatcttcg aggaacgcga
     6181 tagcatgggt gccgcttcca tgtttcattg ggccgggctg gcggataatt ttcacccctt
     6241 tggcgcgcaa ctcatcgcag gtcttgtaga catccggcac gccgagtgcg atatggccaa
     6301 aaccgttgcc tatgtcgtag gaatgcgtat cccaattgta agtgagttcg atcaccgcat
     6361 cggtatcttc ggaaccgtaa ccgacgaaca cgttggtgaa gcggcccgac tcgtagtctt
     6421 tcttgcgaat cagcttcata ccaagaccgt cacagtagaa tttcaagctc tcttcgagat
     6481 tattcacccg gatcatagta tggacgattt tcatgacgtt cctcgctgcc gatagctcgg
     6541 cccttcgtca aattctacac tagcaatttc aaacagtaga ttttctcctt ttaatcgtca
     6601 agcatcgcgc acaccgtgcg atctcgcagc aacagaaagg tctggaccat accggatctc
     6661 ctcgccactg caaaatcgaa aacccttcgg atagataaaa gcgcgttgcc atacccaagc
     6721 tatgggtcaa gccggcagcg agtttacctc gctgctgcgt atgacctcat gctgttggtg
     6781 agaatgaacg acccagtcaa agcgatggcc ctggcgctcg gccgatcaag ccgaagccca
     6841 accttgctgc actaacgcag atttcaccga ctcgatttcc gcctctaacg ccgccagagt
     6901 cgcaggaacc ttcgtcaggt cgcttctctc gccgcactcc tcgatattcc gggccaagcg
     6961 caggacccga ttagccccca cttgccctgc cgacgatccc agtgtgtgaa cggcgccggc
     7021 tagagctgaa gattcctcgg atgatagggc gcgacgtatt tctgccaaca gcaccgggta
     7081 ttgcaccaaa aacatccggc taattttacc gaaaagcact ctgtctccct ccacgtttgt
     7141 caacgcaccg gtaaagttaa atacatcgcc atcattacca tctgacacag gtgctggaga
     7201 tgtctggttc gagccggact tgatcatcgt ccgctcgatg gccgccttca attcccgtgg
     7261 cataatcggc ttcgacacat agtcgtcaaa accccaagcc aaacatttat cccgatcttc
     7321 tttccgggca tgggctgtta tcgcaatcac cgaactttgc agggctgctt gttgtttcag
     7381 tatttggatt tgccggctgg cttcaaaacc gtccatttca ggcatctg
//
LOCUS       DASQKS010000690         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5442324, whole genome shotgun sequence.
ACCESSION   DASQKS010000690 DASQKS010000000
VERSION     DASQKS010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5442324"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VE170_17905"
     CDS             <1..653
                     /locus_tag="VE170_17905"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HYJ17374.1"
                     /translation="GRVLTIQADCEIGGPHLYVEKMLPAAPTKLDQLIVARTAAEEID
                     HFRKVARVAGDMGVDVSFVLSQPNEKRFIESFRGLITTWEDNAVFGFLIDRVGRYQLE
                     EFYDCTYLPLQRILPDIVTEELGHIEYGYNKTCELLSKGDEQKGRVQKAIDYWYVRAL
                     DMFGRSGSKRAERYRYWGLKRRSNEQAREEYIREVNPILLSMGLAVPDPLKGRSYT"
     gene            664..855
                     /locus_tag="VE170_17910"
     CDS             664..855
                     /locus_tag="VE170_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17375.1"
                     /translation="MPQSTANAEPLDQHLLEVVRALHGAVKQGVSHNDDPKIVDQAIA
                     DCKEILDVIMGGNVKDWLN"
     gene            888..1328
                     /locus_tag="VE170_17915"
     CDS             888..1328
                     /locus_tag="VE170_17915"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYJ17376.1"
                     /translation="MSETPEYFYQTEIEWTGDKDIKLSSGKLPAIAAGAPPEFKGREE
                     NWSPEHLFVAAMNSCYVLTLLAIAELSKVSIVSLSSTACGKLEKVQGSGYQITEISVK
                     PRIVISSVNDLTRMPRILEKAKENCFVSNSIKSAVKVEPELLHH"
     gene            1670..2953
                     /locus_tag="VE170_17920"
     CDS             1670..2953
                     /locus_tag="VE170_17920"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ17377.1"
                     /translation="MKSFLSLTPVKAVLIAGGLAFFAAAIWLWLRPTQVTMAEVIVRD
                     ISPVVQGVGTVEAKMVVQIASKIAGRIVMMKVDQGDTVGLGQLLVQLEDSESSAEVER
                     ALANLDRAKLAVHAQQASVLRSQAALSAAEAIVVRAQANRSLAYANAARWDKLAAAEL
                     VAKMDLDERNNAAQVADAELKSAEALREAAVREVAVQNVALNIVPKDIAASAAALASV
                     QAKKADTVITSPIKGYVVSRELESGATVNAGTPILKLADRRTIWVTVYVDEREAGSIT
                     VGNRADVTLRSMPDRAFLGKVVRIRRESDRVTEQLTVDIDFDRPPERLILGEQAEATI
                     RPTTKNAMALPLAALVQSPKGAGAWTVVDGRLRFRRARLGMVDPAGWIEVIAGFADGE
                     HVVIAPGKLADLKNEGRRVTAGAKTSDAIASHKLP"
     gene            2950..>3486
                     /locus_tag="VE170_17925"
     CDS             2950..>3486
                     /locus_tag="VE170_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ17378.1"
                     /translation="MNLAIRDVKYRLGRFILTAIGVGLLLATVMAMGGIYRGMVEDAL
                     SIVRAPGADIWVVQKDTNGPFAETSRIPDDLYRVMRAVPGVREASPIAFQSLQLDYGE
                     ISIRAQIIGYRPGNLGAPPAVAAGRPLVASRYEMVIDRKAGIPLGARLPIGRMSFVVV
                     GLTEGIVSSSGDSTAYVSL"
BASE COUNT          784 a          924 c         1042 g          736 t
ORIGIN      
        1 tcgggcgcgt attaacaatc caggcagact gcgagatcgg cggaccgcat ctatacgtag
       61 aaaagatgct tcccgcggcg ccgaccaaac tggatcaact tatcgtcgcg cgcaccgccg
      121 cggaggagat cgatcatttc cgtaaagtcg cgcgggtcgc cggtgacatg ggcgtcgacg
      181 tctctttcgt gctgagccag cctaacgaaa agcgcttcat cgaatccttc cgcggtctga
      241 tcactacgtg ggaagacaat gccgtgtttg gattcttgat cgaccgcgtt ggccgctatc
      301 agctcgaaga gttttacgat tgcacctatc tgccattgca gcgcatcctg cccgacattg
      361 tgaccgagga gcttggacac atcgagtacg gctacaacaa aacctgcgaa ctgctctcaa
      421 agggggacga gcagaaaggg cgggtgcaaa aggcgatcga ttattggtat gtgcgggcgc
      481 tggatatgtt cggtcgaagc ggatcgaaac gcgccgaacg ctatcgctat tggggtctca
      541 aacgacgtag caacgagcag gctcgggaag agtatattcg ggaagttaat ccgattctac
      601 tgagcatggg actggcagtt ccggatcctc tcaaaggtcg tagctacact taaggaggtt
      661 tttatgccgc aatcaacagc caacgccgaa ccgctcgatc agcatcttct cgaagttgtg
      721 cgtgccctgc acggcgcggt aaaacaggga gtgtctcaca acgatgatcc gaaaatcgtc
      781 gatcaagcga tcgcggactg caaagaaatc ctcgatgtca tcatgggcgg aaatgtgaag
      841 gattggttaa attagaccga tcccagccat taataaaggg atgcccaatg agtgaaacgc
      901 cagagtattt ttaccagaca gagatcgagt ggacaggcga caaggacatc aagctgagca
      961 gcggcaagtt gccggcaatc gccgccggcg cgccgcccga attcaaaggg cgggaggaaa
     1021 actggtcgcc ggagcatctt ttcgtcgccg cgatgaacag ttgttatgta ctgactctgc
     1081 tcgcaattgc ggaattatcc aaagtgtcga tcgtgagttt gtcctcgact gcctgcggga
     1141 aattggaaaa agttcaagga agcggctatc aaatcaccga gatctccgtg aaaccgagaa
     1201 tagtcatctc gtcggtgaac gatctgacgc gcatgccgcg aatcttggaa aaggccaaag
     1261 agaattgctt cgtctcgaat tcaatcaaaa gcgccgtcaa ggtggagcca gagctccttc
     1321 atcattagct gccaacgtct tcgtatcagc tgggcggagc ttaggactct cccactttca
     1381 ttccattagg cagtttgccg ggatctgccg ctttctcact tacgcaaaac caacttgctc
     1441 gtttcgcgct cccgggaaaa ttagagagag gtcttaaaga cgcatcggtc tcttattgac
     1501 agtaatctta acctgtccgc aggtcgtcaa ttatagaacc agggcgtgac cggtctcgat
     1561 tcgcgcggca catccattgc tctttcaggg gccagcgggg caattcattc ttgccgggta
     1621 gtgccagaat ccatagtcac gcggaaagcc gtacgagagc gcaagtatca tgaaaagttt
     1681 cctgagtttg actccagtta aagcagttct tattgccgga ggtctggctt tttttgcagc
     1741 ggctatctgg ctctggctgc gaccgaccca ggtgactatg gctgaagtca tcgttcgcga
     1801 tatcagtccg gtggttcagg gcgtgggaac ggtcgaagct aagatggttg tccagattgc
     1861 gtccaagatc gcagggcgaa tcgttatgat gaaagttgat cagggcgaca ctgtgggctt
     1921 gggccagcta cttgttcaac tggaagattc cgaatcgagc gccgaggtcg agcgggcgct
     1981 ggctaactta gatagagcca agctggccgt gcacgcacag caggcatccg tgctccgatc
     2041 ccaggcggcg ctatcggccg ctgaagcgat cgtcgtcaga gcgcaggcga accggtcgct
     2101 tgcctacgcc aatgcggcgc gatgggataa actcgccgcc gctgaacttg tcgcaaaaat
     2161 ggatctggac gaacgcaaca acgctgcgca ggtggccgac gctgagttga aaagcgccga
     2221 agcgttgcgt gaagccgctg tgagagaggt cgccgtccag aacgtcgcat tgaacatcgt
     2281 gcccaaggat atcgccgcat cggcggctgc gctcgcctca gttcaagcaa aaaaggcgga
     2341 taccgtcatc accagcccga tcaaaggcta cgtggtaagt cgcgagctcg aatcaggagc
     2401 aacggtcaat gccggcacgc cgattcttaa gctcgctgat cggcgcacca tctgggtcac
     2461 ggtctacgtt gatgagcgag aagccggctc gattacagtc gggaacaggg ctgacgttac
     2521 gctgcgctca atgcccgatc gcgcattcct cggcaaggtt gtcagaattc gccgcgaaag
     2581 tgatcgcgtc accgagcaac tcaccgtgga catcgacttc gatcgcccac ctgaacgcct
     2641 tattctgggc gaacaggctg aagcgaccat ccgtcccact accaaaaacg caatggcctt
     2701 gccattagcc gcgttggtgc agtcacccaa aggagcgggc gcgtggacag ttgtagatgg
     2761 ccggttgcga ttcaggcgcg cgcgtctggg catggtagat cccgctgggt ggattgaagt
     2821 tatcgcaggg ttcgccgacg gcgagcatgt ggtcatcgcg ccggggaaac tggctgatct
     2881 gaagaacgag gggcgtcgcg tgaccgctgg cgctaaaacg tcagacgcga tcgcgtcgca
     2941 taagttgccg tgaatctggc tattcgcgac gtaaaatatc gcttggggag gtttatcctg
     3001 accgccatcg gtgtcgggtt gttgctggcg acggtcatgg ccatgggagg gatttatcgc
     3061 ggtatggtgg aagacgccct cagcatcgtg cgcgccccgg gcgctgatat atgggtggtg
     3121 caaaaggaca ccaacggccc cttcgcggaa acctcgcgca tcccggatga tctttaccgt
     3181 gtgatgcgcg cggtgcctgg ggtacgcgaa gctagtccga ttgcgtttca atcgcttcag
     3241 ctcgactacg gcgaaataag cattcgcgcg cagattatcg ggtaccgccc tggcaatctc
     3301 ggcgcgccgc ctgcggtggc agcgggtcgt ccgctggtgg cgagccggta cgagatggtg
     3361 atcgatcgga aggctgggat tcccttagga gcgcgattgc cgatcggtcg gatgtcgttc
     3421 gttgtcgtcg gcctgaccga aggcattgtt tccagctccg gggattcaac cgcctatgtt
     3481 tcgctg
//
LOCUS       DASQKS010000691         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5848610, whole genome shotgun sequence.
ACCESSION   DASQKS010000691 DASQKS010000000
VERSION     DASQKS010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5848610"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VE170_17930"
     CDS             complement(<1..295)
                     /locus_tag="VE170_17930"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYJ17379.1"
                     /translation="MANLGFVGLGLMGSRMAQRLLAAGHSVTGYNRTKSKAQRLLDAG
                     MKWADSPRAAAAASEMVFTMVANTEALRAVTEGADGILAGLSAGKIYIDMSTVS"
     gene            complement(414..1427)
                     /locus_tag="VE170_17935"
     CDS             complement(414..1427)
                     /locus_tag="VE170_17935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYJ17380.1"
                     /translation="MKIARQLTAYSFLTWSIFTVAPAFGQANFYDGKAVTILIGAKSG
                     SLEIAALIVAHHLGKYIPSKPAVILQHMPGAAHLLATNNVFNVAKPDGLTILAANPNV
                     AIAQMSKIPQARFDIRKFHWLGSSGADGVMFSIRPDLPYKSFDDLKKADRELVAGTTG
                     PGSNAHDFPLLLKEFAGAKLKLVAGYPSNSDVLLAVERKEVDAWSALATTIKLAADRG
                     AVRPLVRGRTALPGYENLPVDEDLATNALGKSLMSIKSIPQAIGRAFAVHPNTPKDRL
                     VMLREAFAKAIKDPELLAEAKRARIDFSYISAEQVTKDFNALFNQTPETLKEMGKYIK
                     AES"
     gene            complement(1529..2005)
                     /locus_tag="VE170_17940"
     CDS             complement(1529..2005)
                     /locus_tag="VE170_17940"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYJ17381.1"
                     /translation="MHRGRILFCAFLILVSATAIYLALDWPFKAALFPLAVSIPLLIL
                     AASQLRQLFLGKAETIESAAVDLEFSSDVPPEIERRRVITAFSWIAGFILCVYLIGFP
                     LTVPLFICCYLRFESGVGALPTIAATAITWMVFYGLFQKLVHLQFEQGVVQAWLGL"
     gene            complement(1998..>3026)
                     /locus_tag="VE170_17945"
     CDS             complement(1998..>3026)
                     /locus_tag="VE170_17945"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYJ17382.1"
                     /translation="AGARGQVKGFAMGLCGLLVSTIGQERQSGSLRFDMGLMYLWEGL
                     DFVPVLVGVFAIPEIIDLTVRGTAIAGERPAENLRSGVIEGIKDTFRHFWLVFRCSVV
                     GVFVGILPGAGGGVAQWMAYAHAVQGAKDAKDRDRFGKGDVRGVLGPGAANNSKEGGE
                     LIPTVAFGVPGSGAMAILLSAFLIMGLSPGPDMLTKYLPITFSMVWTLVIANIIAVLL
                     CLTILDQLAKVTNVRGGLIIPFVLLLVFVGSYTANGQITDLLVTLIFGVLSYFMVLFG
                     WPRPPFVLGFVLGKVIETYFFISVARYGFAWLRQPMVIFLIILMLFVIAYPYIQKRRQ
                     RVQSQTNA"
BASE COUNT          745 a          857 c          844 g          580 t
ORIGIN      
        1 gactgaccgt gctcatatcg atatagattt tgccggcgga aagcccggcg agaatgccgt
       61 ccgcgccttc ggtgaccgcg cgcaacgctt cggtgttggc gaccatcgtg aacaccattt
      121 cgctcgccgc ggcggcagcg cgcggactgt cggcccattt cattcccgcg tcaagcaagc
      181 gctgcgcttt ggacttggtc cggttataac ccgtgacact gtggccggca gcgagcagcc
      241 gttgcgccat gcggctgccc atcaatccga gaccgacaaa gccaagattt gccatcagcg
      301 actccagtaa aaaattcgcg acaaactaaa cgactaacga atctccaatc cgggaaaaga
      361 tccgcatcgg aattgtttgg ggaccggaat gctttcgatt tcgcgtttgc cgattaactt
      421 tccgccttga tgtacttgcc catctctttc agcgtctcgg gcgtttggtt gaacaaagca
      481 ttaaaatctt tggtgacctg ttcggcggag atgtaagaaa aatcgatgcg cgctcgcttg
      541 gcttcagcga ggagctccgg gtccttgatc gccttggcga acgcctcgcg caacatcacc
      601 aaacgatcct taggcgtatt tgggtgaacg gcaaaggcgc gtccaatcgc ttggggaatt
      661 gatttgatcg acatcagcga cttgcccagc gcgttggttg cgagatcttc atccaccggc
      721 agattttcat aaccaggcaa agccgtgcgg ccgcgcacca gcggcctgac ggcgccacgg
      781 tcggcggcga gtttgatcgt cgttgccaac gctgaccagg cgtccacttc tttccgctcg
      841 acggccagca gaacatcgct gttggatgga taaccggcga ctaattttaa tttggcgccg
      901 gcgaattcct tcagcagcag gggaaagtca tgggcgttgg agcctggccc cgtggtaccg
      961 gcaaccagtt cgcggtcggc cttcttgaga tcgtcaaaac ttttataagg aaggtctggg
     1021 cggatcgaga acatcacgcc gtcggcgccc gaggagccga gccagtgaaa cttccgtatg
     1081 tcgaagcgcg cctgcgggat cttcgacatc tgcgctatgg cgacattcgg attggctgcg
     1141 aggatggtca agccatccgg tttggcgacg ttgaatacgt tattggtcgc caacagatgg
     1201 gcagcgccag gcatatgttg caagatcacc gccggtttgc tgggaatata tttgcccaaa
     1261 tggtgcgcga cgatgagcgc cgcgatttca agactgccgc tcttggcgcc gatcaaaatg
     1321 gtgacggctt tgccatcgta aaaatttgcc tggccaaatg ccggcgcgac tgtaaaaatc
     1381 gaccaagtca aaaaggaata agccgttaac tgccgagcga tcttcatatt atcctcccgc
     1441 aacgcgttta gcttcatcca gcaattttac cgaagagcga cgcaagggta tgtgattcta
     1501 acgacgccag tcaagtcaat gaccacgatt aaaggccaag ccacgcctgc accacgcctt
     1561 gttcgaattg caagtgcacg agtttctgaa acaggccata aaacaccatc caggtgattg
     1621 ccgttgccgc aatcgtcggc agcgcgccga cgccgctttc aaagcgaagg tagcagcaaa
     1681 taaacagcgg cacggtgagc ggaaagccga tcaggtaaac gcaaagaata aaaccggcga
     1741 tccaagaaaa cgccgtgatc acgcgtcgcc gctcgatctc gggcgggacg tcgctggaga
     1801 attccaggtc gaccgccgca ctctcgatag tttccgcttt gccgagaaac agttgccgga
     1861 gctggctcgc ggccaggatc aaaagtggaa tacttaccgc caacggaaac aaagccgctt
     1921 taaacggcca atcgagcgcc aggtagatcg ccgtagcaga gactaagatg aggaaagcgc
     1981 agaagagtat gcggccccta tgcattcgtt tggctctgga cgcgctggcg ccgcttctgg
     2041 atatacggat aagcgatcac aaagagcatg agaatgatta gaaagataac catcggctgt
     2101 ctgagccagg caaagccgta gcgcgcgaca gatataaaaa aataggtctc gatgactttg
     2161 cccaagacga agccgagaac gaagggtgga cggggccaac cgaacagcac catgaaatag
     2221 ctgagcacgc caaagatcaa ggttacgagc agatcggtga tctggccatt ggcggtataa
     2281 ctgccgacaa aaaccagcag caaaacgaag ggtataatca aaccgccgcg cacgttggtc
     2341 actttggcca gttgatcgag aatggttagg cacaagagca cggcgatgat gttggcgatc
     2401 accaaagtcc agaccatcga gaaggtaatc ggcagatact tggtaagcat gtcgggtccg
     2461 ggagatagac ccatgatcaa aaaagcgctg agtaagatcg ccatggcgcc gctgccggga
     2521 acaccgaagg ctacggtcgg gatcagctcg ccgccttctt ttgaattgtt cgccgcccca
     2581 ggtccgagta caccgcgcac atcgcctttg ccgaagcggt ccctgtcctt ggcatctttg
     2641 gcgccttgca cagcgtgagc ataggccatc cactgggcga cgcccccgcc ggcgccagga
     2701 aggatgccga cgaacacgcc aaccaccgag cagcgaaaca ccagccaaaa atgacgaaag
     2761 gtatctttga tgccttcgat cacgccgctg cgcaaattct ccgccggccg ctcccccgcg
     2821 atcgcagtgc cgcggacggt aagatcgata atttccggga tcgcgaagac accgaccaac
     2881 actggcacga agtcgagacc ttcccacaaa tacatcaagc ccatgtcaaa acggagggac
     2941 ccggactggc gctcttggcc aatggtcgac accagcagac cacacagacc catggcgaaa
     3001 cccttgacct gtccgcgcgc gccggc
//
LOCUS       DASQKS010000692         4579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5443432, whole genome shotgun sequence.
ACCESSION   DASQKS010000692 DASQKS010000000
VERSION     DASQKS010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5443432"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VE170_17950"
     CDS             <1..595
                     /locus_tag="VE170_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17383.1"
                     /translation="EFAYPSLTSVVPASVGIVKGGPNPQEARAFVNYLLSEAGQTVLF
                     TPEIARLPVIPELYAKAPKDYPNPFKKKLGGVEFNDELSSSRRDVVNSLYDHIVTFRH
                     NELREAWKSIYRAEDALAKSKTANTAQGKGMIADARKLASQVPISDKRAADKEVTGAF
                     KSKSGLKSQLETEWENAARGNYSKAKELAEKAAALAK"
     gene            748..2454
                     /locus_tag="VE170_17955"
     CDS             748..2454
                     /locus_tag="VE170_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ17384.1"
                     /translation="MALTTTAVTAPPRQSIDWWQGGIMASLLAILTAFILYPVLRVLW
                     ISVTDDQSNLTLIHFGNFFRRPLFREALWNTLWSGFLVVVFSSLLALPLAYVLARYEF
                     RGKLLLQTAATLPLVIPPFVGAVALQLILGRSGMVNLLLRDWFDVSIPFMEGLTGVVM
                     VQTLHFFPFILLNTVVSLSNIDASLEEMAQNLGCHGLRLFRRVTLPLMLPGFIAGALL
                     TFIRAIDDLGTPLMLNYKNLLAPQAYLRITTIGMDDVDGYVVCVVLVVLSMVTLLAAR
                     KYLSLSEYATVQRAAPLSRQLHGKQLTLVWLIIAVILGISLLPHIGIVLLSFAKVWSF
                     SLLPSTYTLGHYEEILLRVPHFVINTLIYTLLAAALDIVLGAAIAFLLLRTGVPGRNF
                     LDAIATMPLAIPGVVLAVGYLRVFHGWDFPGLGAPLTSSWLILVVAYTMRRLPYTVRA
                     CYAALQQVHITLEESAQNLGANRLRTFIKITLPMISGGLVAGGLIAFVTSCVELASTI
                     MLVPRIELGPISYGIYLYMQSPLGRGAGAALGVVAILLVSLGTYLTHRVFGARAGNAF
                     RL"
     gene            2457..3554
                     /locus_tag="VE170_17960"
     CDS             2457..3554
                     /locus_tag="VE170_17960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ17385.1"
                     /translation="MKPAQVTLENIGKCYGQQWVVRDVNLQIQAGDFFTFLGPSGCGK
                     TTLLRMIAGFAIPDRGSIHLDDQEISRVPPWQRDIGMVFQNYALWPHMTVFENVAFGL
                     RERKVPRGEILTRVNQALATVDLKGRESHRPSQLSGGQQQRVALARILVIQPRVVLLD
                     EPLSNLDAKLRIEMRLELLKLQRDLGLTTIYVTHDQGEALAMSTKIAVINHGQVVQVG
                     PPRAIYEQPIDNFVAAFVGQANLLPGAIRSRSDHTYAVDIGDGNVVLAHAIGLTFQAG
                     DAVSLSLRPEAVELSPCEAEKVSLNALQGKIIAAAYQGAVVEYEVSAVGTTLKVRVAN
                     ATGQTLYQRGDAVCLSFAPEAVTCLAGFSNK"
     gene            complement(3590..3994)
                     /locus_tag="VE170_17965"
     CDS             complement(3590..3994)
                     /locus_tag="VE170_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17386.1"
                     /translation="METEQSIPKKSAPVVIEAAGMRIWVDYRRGIGGDEGLTFDVTTV
                     TGEEEGARILRFDCFSKTPHYHVGASPKSPVHDMLAEGITDPVRWTLEQLKTRLPSMV
                     SEAGYDDIAKAIDQQAIAQSLARVEPDIFAKI"
     gene            complement(4182..>4579)
                     /locus_tag="VE170_17970"
     CDS             complement(4182..>4579)
                     /locus_tag="VE170_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HYJ17387.1"
                     /translation="AFAELLHKRARAEWGYGKNERLTPEDLIQEKYRGIRPAPGYPAQ
                     PDHTEKPAIFDLLQVGEATGITLTESFAMYPASSVSGLYFAHKDAHYFAVNSIGKDQV
                     EAYAARKGMKVSEVERWLSPILGYDPAKQ"
BASE COUNT          879 a         1413 c         1280 g         1007 t
ORIGIN      
        1 ggagttcgcc tacccgtcgt taacctcggt agtgccggcc agcgtcggca tcgtcaaagg
       61 cggccccaat ccgcaggaag cgcgcgcctt cgtcaattat cttctcagcg aagccggaca
      121 gaccgtgttg ttcacccctg agatcgcgcg attgccggtg atcccggagc tctacgccaa
      181 ggcgcccaag gactatccca atccgttcaa gaaaaaactc ggaggggtcg aattcaacga
      241 cgagctgtcg tcgagccgcc gcgatgtagt gaattccctg tatgaccaca tcgtaacatt
      301 ccgccataac gaattacgcg aagcctggaa gagcatttac cgggcggagg acgcgctggc
      361 aaaaagtaag acggccaata ccgcccaggg caaagggatg atcgccgacg cgcgcaaact
      421 cgcatcccag gtgccgatca gcgacaagcg cgccgccgac aaggaagtca ccggcgcctt
      481 caagtccaaa tcgggcctca agtcccaact cgaaaccgaa tgggaaaacg ctgcccgcgg
      541 caactacagc aaagccaaag agctggcgga aaaagccgcg gcattggcga agtaatttca
      601 cgggagccgc gtaaagcggg ctgagccacg acaaatggaa cccgtcattc accgcgaagg
      661 cacgaagttc acgaagggtt ccaagaagca aaaatccgaa cttcgtgttt ttcgtgcttt
      721 cgtggtgaat aataactccc tccgcctatg gcgttaacca caaccgcggt cactgcgccg
      781 ccgcgccaat ccatcgactg gtggcaaggc ggcatcatgg cgagcctcct ggctatcctc
      841 accgcgttca ttctttatcc cgtgttgcga gtcctgtgga tttccgtcac cgacgatcag
      901 agcaatttga ccctgattca cttcggcaac ttttttcgcc ggccgctgtt ccgcgaggcg
      961 ctgtggaata cgctatggag cggtttcctc gtcgtcgttt tcagttctct cctggctttg
     1021 ccgctggcct acgtgctggc gcgctacgag tttcgcggca agctgctgct gcaaaccgct
     1081 gcgaccctgc cgctggtgat cccgccattt gtcggcgcgg tcgcgctgca attaattttg
     1141 ggccgcagcg gcatggttaa tctgctatta cgagactggt tcgatgtctc gatcccgttc
     1201 atggaaggcc tcaccggcgt cgtcatggtt cagacgctgc acttttttcc gttcatactg
     1261 ctcaacaccg tggtgtcgct gtcgaacatc gacgcctccc tcgaagagat ggcgcagaat
     1321 ctcggctgcc acggcctccg tttgtttcgt cgggtgactt taccgctgat gctgccgggc
     1381 ttcatcgccg gtgcgctgtt gacgttcatc cgcgccatcg acgacctcgg cacaccgctg
     1441 atgttgaact acaaaaacct gctggcgcct caggcttatc tgcgcatcac caccatcggc
     1501 atggacgatg tcgacggcta cgtggtctgc gtcgtgttgg tcgtgctttc gatggtaacg
     1561 ctcctggcgg cgcgcaaata cttgagcctc tccgaatacg ccacggtgca acgcgccgcg
     1621 ccgttgtcac gccaattgca cggcaagcaa ctcacgctag tttggctcat catcgcggtc
     1681 atcctcggca tcagcctgct gcctcacatc ggcattgttc tgctgtcgtt tgccaaggtc
     1741 tggagctttt cgctcctacc ctcgacctat actctgggcc attatgagga aatcttgttg
     1801 cgcgtgccgc acttcgtcat caacaccttg atctatacgc tgctcgccgc tgccctcgat
     1861 atcgtgctcg gcgccgccat cgcgttccta ctactgcgca ccggcgtgcc gggccgcaat
     1921 tttcttgacg ccatcgccac catgccgctg gcgattcccg gcgtggtgct ggcggtgggt
     1981 tatctgcgag tatttcacgg ctgggacttt cccggtcttg gcgcgccgct gacgtcatcg
     2041 tggctgatac tcgtggttgc ctacaccatg cgccgcctgc cctacacagt gcgcgcctgc
     2101 tacgccgcgc tgcagcaagt tcatattacg ctggaagaat cggcgcaaaa cttgggcgcc
     2161 aaccgtttgc gcacctttat caagatcact ttgccgatga ttagcggcgg cctggtcgcc
     2221 ggcggactga ttgctttcgt aacttcctgc gtggaattgg cctcgaccat catgctcgtg
     2281 ccgcgcatcg aattggggcc gatctcctat ggcatctatc tctacatgca gagcccgctc
     2341 gggcgtggcg ccggcgcggc tttgggcgtg gtcgccatcc tcttggtttc ccttggcacc
     2401 tatctcaccc accgggtttt tggcgcccgc gccggcaacg cttttcgcct gtaaacatga
     2461 aacccgctca agtcacactg gaaaatatcg gtaaatgcta cggccaacag tgggtggttc
     2521 gcgatgtcaa tctgcagatt caagccggcg atttcttcac cttcttgggc cccagcggct
     2581 gcggcaagac gaccttgcta cgcatgatcg ccggcttcgc gatcccggat cgaggatcga
     2641 ttcacctgga cgaccaggag atcagtcgcg tgccgccatg gcagcgcgac atcggcatgg
     2701 tgtttcagaa ttacgctctc tggccgcaca tgacggtgtt tgaaaacgtc gcgttcggcc
     2761 tgcgcgagcg aaaagttccc cgcggcgaaa tccttacccg ggtcaaccaa gccttggcga
     2821 ccgtcgacct caagggccgc gagtcgcacc ggccatcgca gctctccggc ggccagcagc
     2881 agcgcgtcgc cctggcgcgc atcctggtga ttcagccgag ggtggtgttg ctggacgaac
     2941 cgctcagcaa tctcgacgcc aagctgcgta tcgagatgcg cctggaatta ttgaagctgc
     3001 aacgcgacct cggactgacg acgatctacg tcacccacga tcagggagag gcattagcga
     3061 tgtcaacgaa gatcgccgtg atcaatcacg gccaagtcgt tcaagtggga ccgccgcgtg
     3121 ccatctatga gcagccaatc gacaacttcg ttgccgcctt tgtcggccaa gccaatttgc
     3181 tcccaggggc aatccgctcg cgcagcgacc acacatacgc ggtcgatatc ggcgatggca
     3241 acgtcgtcct agcccatgcc atagggttaa cgtttcaagc cggcgacgcc gtgtcgctta
     3301 gccttcggcc ggaagcggtc gaactatcgc cctgtgaggc cgagaaagtg tccctcaatg
     3361 ctcttcaggg aaagataatt gcggccgctt atcaaggcgc agtcgtcgaa tacgaagtca
     3421 gtgcggtggg aactacccta aaagtccgtg tcgccaatgc gacagggcaa accctttatc
     3481 agcgaggcga tgccgtatgc ctatcttttg ccccagaggc ggtcacttgc ctggcgggct
     3541 tcagtaataa ataaccatcc gagtaagcgg caacgaaatt gttacgccgt taaattttgg
     3601 cgaagatgtc tggttcgacc cgcgccagac tctgggctat cgcttgttgg tcgatcgcct
     3661 tggcgatgtc gtcataacca gcttcggaaa ccattgacgg cagtcgggtc ttgagctgtt
     3721 cgagggtcca gcgcaccgga tcggtgatcc cttcggcgag catgtcatgg accgggctct
     3781 tcggtgatgc gccgacgtgg taatgcggcg ttttgctgaa gcagtcgaag cgcaggattc
     3841 tcgcgccctc ctcttcgccc gtcacggtgg tgacatcgaa ggtcaacccc tcatcgccgc
     3901 cgatgccacg gcggtagtcc acccaaatcc gcattcccgc cgcctcgatg acaaccggag
     3961 cgctcttttt cgggatcgat tgttctgttt ccatggtccg cgtcctttca ggtttgatga
     4021 gtcaatccgc cgagccagcc cagcgacatt aacagcctcc cggccgcttg cgcaaaccga
     4081 ttaagcgatg gcgaacggag aagcgggcca atttgcttac ggcttttcgg cgacgccctt
     4141 gccgtccttc cagagtgact tacctgtgaa tcgagacccg ctcactgctt cgccggatcg
     4201 tagccgagta tcggcgacag ccaccgttcc acttcagaaa ctttcatccc tttgcgcgcg
     4261 gcgtaggcct cgacctgatc tttgccaatc gaattgacgg cgaagtagtg cgcgtctttg
     4321 tgggcaaagt agaggccgct caccgatgac gccggataca tggcgaaact ttctgtcagt
     4381 gtaatgccgg tggcctcgcc cacttggagc agatcgaaga tcgccggttt ctcggtgtgg
     4441 tcgggctgag cgggatagcc aggcgccggg cggatgccgc gatatttttc ttgaatcaaa
     4501 tcttccggag tcaatcgctc gtttttgcca taaccccact cggcgcgggc tcgcttgtgc
     4561 aagagttcgg caaaggctt
//
LOCUS       DASQKS010000693         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4568409, whole genome shotgun sequence.
ACCESSION   DASQKS010000693 DASQKS010000000
VERSION     DASQKS010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4568409"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VE170_17975"
     CDS             <1..1251
                     /locus_tag="VE170_17975"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ17388.1"
                     /translation="TMVQYASKLTFGPETADWQERLNSERMRNQRVARAQTIMKKHGV
                     AALLEANHHNIRYLTALRGFTYPMCRYVLFFAEHDPVMYEHDGYFHQMPDQCPWIKEW
                     RPAHSWLSGAPGPGACADEAKAFAGEIKAELEKRGLLAEKLALGSFDGNAREALVSAG
                     VKNLVDSRNLMLEARMIKNQDEINCLKTCASMVDGVWYRVWENLKPGIRDTDLMRIAS
                     GAGWEMGLEGSVPGGWRTGPTTFDRGPHATSRVIQFGDMVYGSYCGATYMGYGSCTYR
                     TFIVGRKPTAKEQDWYKEVRERLDLVISEIKPGRSTADAAKHFPPATKWGYRSEVEIL
                     ASEIGHGIGLGTNTGYDIPIINRLWSFDHPQVFEEGMTLAVESREGETRVGGARLENM
                     LVVTKNGAEIMDYFPRDEILVAPR"
     gene            1436..2629
                     /locus_tag="VE170_17980"
     CDS             1436..2629
                     /locus_tag="VE170_17980"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ17389.1"
                     /translation="MPVKILDNITVIECATFVTGPYATALLADLGARVIKIESAPDGD
                     PYRYFAPDPAFSFNFAHLNRNKESLVLDLKAPAGKAICLELLANADVFVENFRPGTAK
                     RLGIGYETVQAINAGLVYCSISAFGQSGPYAEKPGFDTLGQAMSGLLSLLSDPNDPKV
                     MGMAVSDYVTGLSAGYGILAALLARTNSGAGCRVETSLLQATLSFIGETAAGYLRTGN
                     VPNRMARVKNAHAFAVVCQDGKPLAIHCSVPEKFWLALLQSVERMDVAADERFKTRDG
                     RRQNYQALEQTLAPAFKTKTRGDWLKILDAHDVPAAPLYDVAEVLDDPQVKHLGLIEE
                     VEHPKAGKLKFVGGPVRFDDLAKDKAQPPPLVGEHSERILRELGHNDAAIQQLVTQGV
                     SQIAG"
     gene            2667..>3141
                     /locus_tag="VE170_17985"
     CDS             2667..>3141
                     /locus_tag="VE170_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYJ17390.1"
                     /translation="MNRLFTAIGFLLLCLCMALTTASQAQLIAGTTPEIFKQFSEHVV
                     KIEVVETSSAAKASTGTGFAADNSGRIVTNYHVISKLVHSPERYRIDVIDVAGGTTVG
                     TVLGVDVVYDLAILRTQRQPKGHLVFESNPVAQGTRLYSLGHPRDLGLSIVEGTHN"
BASE COUNT          653 a          905 c          960 g          623 t
ORIGIN      
        1 accatggttc aatatgcatc gaaactgact ttcggtccgg aaaccgcaga ttggcaagag
       61 cggctcaata gcgaacgcat gcgcaaccag cgcgtggcgc gggcgcaaac gatcatgaaa
      121 aaacatggcg tcgccgcgct gctcgaagcc aaccatcaca acattagata tttaacggcg
      181 ctcaggggct tcacttatcc catgtgccgt tacgtgttgt tcttcgccga gcacgatccg
      241 gtgatgtacg agcatgacgg ctattttcat cagatgccgg accaatgccc atggatcaaa
      301 gagtggcggc cggcgcactc ctggttgagc ggcgcgcccg gccctggcgc gtgtgccgac
      361 gaggccaaag cgttcgccgg cgagatcaaa gctgaactgg agaagcgcgg cctgttggcc
      421 gagaaactgg cgctcggcag tttcgatggc aacgcccgcg aagcgctggt cagcgccgga
      481 gttaaaaacc tcgtcgactc gcgcaacctc atgctcgaag cgcgcatgat caaaaaccag
      541 gatgaaatta actgcctcaa aacctgcgcg tcgatggttg acggcgtctg gtatcgcgtc
      601 tgggaaaact tgaagcccgg catccgcgat accgatctga tgcgtattgc ctccggcgcc
      661 ggctgggaga tgggactgga aggttccgtg cccggcggct ggcgcaccgg gccgacgacg
      721 tttgaccgtg gtccccacgc caccagccgg gtgatccaat tcggcgacat ggtctacggc
      781 tcttactgcg gcgcgacgta catgggttac ggcagctgca cctaccggac tttcatcgtc
      841 ggcagaaagc cgacggcgaa agagcaagat tggtacaaag aagtgcgcga gcgactcgat
      901 ctggtcatct ccgaaatcaa gccgggcagg agcacggcgg acgcggcgaa acattttcca
      961 ccggcgacca agtggggcta ccggagcgaa gtagaaatcc tcgccagcga aatcggccac
     1021 ggcatcggcc tcggcaccaa caccggctac gacatcccga tcatcaaccg gctgtggtcc
     1081 ttcgatcatc cgcaggtgtt cgaggaaggc atgaccttgg cggtggaatc gcgtgaaggc
     1141 gagacccggg tcggcggcgc gcgtttggaa aacatgctgg tggtgaccaa gaatggcgcg
     1201 gaaatcatgg attactttcc gcgcgatgaa attttagtcg cgccgcgctg agcggtcgca
     1261 agtccggctc gaatcgcacg gcttagaacc aagtcgcgct gtcgccggct taaagcggac
     1321 ggccgtagat ttcctagatt ttgactttgg cgccggcatt tatgggagaa ctcttttgcg
     1381 cgaagcgcgt tgcatgccgc gctgatccat ggaagattag cgcggaaaga tattcatgcc
     1441 ggttaaaatc ctcgacaaca tcacggtcat cgagtgcgcg accttcgtta ccggtccgta
     1501 tgctacggca ctgctcgcag atcttggcgc gcgggtaatc aagatcgaat cggctcccga
     1561 tggcgatcca tatcgttact tcgcgcccga tccggcgttc agtttcaact tcgcgcatct
     1621 caatcgcaac aaagagagtt tagtgctgga cttgaaagct ccggccggca aggcgatctg
     1681 ccttgagctg cttgcgaacg ccgatgtctt cgtggagaac tttcgccccg gcacggcgaa
     1741 acggctgggc attggctatg agacggtgca agcgatcaat gccggactag tctactgctc
     1801 gatctcggcg ttcggccaga gtggacccta tgccgagaag cctggcttcg atactttggg
     1861 ccaggcgatg agcggattat tgagcctgct cagcgatccc aacgatccca aagtcatggg
     1921 catggcggtg tcggattatg tcaccggact ttccgcaggt tacgggattc tcgccgcgct
     1981 cttggcgcgg acaaattccg gggccggatg ccgggtcgag acgtcgttgt tgcaggcgac
     2041 gctgtcgttc atcggcgaaa ccgcggcggg ttatcttcgt accggcaacg tgcccaaccg
     2101 catggcgcga gtgaaaaatg ctcacgcgtt cgcggtggtt tgccaggatg gcaaaccctt
     2161 ggcgattcat tgttcggtgc cggagaagtt ttggttggcg ctcttgcagt ctgtcgagcg
     2221 catggacgtc gccgcggatg aacgtttcaa gacccgcgac ggtcgccgac aaaattacca
     2281 agcgctggaa caaaccctgg cgccggcatt caaaaccaag acgcgcggcg attggttaaa
     2341 aattctcgac gcgcatgacg tgccggcggc gccgctttac gatgtcgccg aggtgcttga
     2401 cgacccgcag gtgaagcatc tcgggctgat cgaagaggtc gagcatccca aagccgggaa
     2461 actgaagttc gtcggcgggc cggtgcgttt cgatgatctg gccaaggata aggcccagcc
     2521 gccgccgctg gtgggtgagc atagcgagag gattcttcgc gagctaggcc acaatgacgc
     2581 cgccattcaa cagttggtga cccagggcgt gagtcaaatc gctggttagc ttgcgccaac
     2641 tgcgcacgaa gtttcctgtc catccaatga atcgattgtt caccgccatc ggctttttgc
     2701 ttctgtgctt gtgcatggcg ctcacgacgg cgtctcaagc gcagctcata gccggcacca
     2761 cgccggagat cttcaaacag ttttccgagc atgtggtgaa gatcgaagtg gtggaaacca
     2821 gttcggcggc gaaggcatcc acgggcacgg gttttgccgc cgacaactcg ggacgcatcg
     2881 tcaccaatta ccatgtgatt tccaagttgg tgcactcgcc ggagcgctat cggatcgatg
     2941 tgatcgacgt cgccggcggc accaccgtcg gcacggtgct gggcgtcgat gtggtctacg
     3001 acttggccat cttgcggacc cagcgtcagc ccaagggcca tctcgtcttc gagtccaacc
     3061 cggtggcgca gggcacccgg ctctattccc tcggccatcc acgcgacctt gggctttcca
     3121 tcgtcgaagg cacgcacaac g
//
LOCUS       DASQKS010000694         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2935112, whole genome shotgun sequence.
ACCESSION   DASQKS010000694 DASQKS010000000
VERSION     DASQKS010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2935112"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VE170_17990"
     CDS             <1..197
                     /locus_tag="VE170_17990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17391.1"
                     /translation="EDLPYILQYVGEDNLIVGSDYTHRDPSMELEFRTLLQQRADRGE
                     IPKTAVEKILYDNPKAFYGL"
     gene            403..1491
                     /locus_tag="VE170_17995"
     CDS             403..1491
                     /locus_tag="VE170_17995"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17392.1"
                     /translation="MRIIDADTHIDETDATWEFMSGGDLAYKPTTAFPSNPDPKLPPA
                     RYWVIEGKRQVRFIRSDKDSGTTVETRELLDIDARLKHMDELGTDIQVIYPTLFLMEA
                     SENPEASTATRKSYNRWLGERCSHSGGRLRWICMPPVQKIDSVLEELTWAKEHGACGV
                     VKKGDREAGKYPADPYFEPLYAAAEKLDLPICIHTGSGIPDFSPAREFSHGQFMRTKG
                     AAINGVLGLVLHKIPMKFPKLRIGCIEAGSMWTPFVAYDLKRQAIKQQGRESVGVLGV
                     PLIDIPTNVFKDNRIWVTCQVDEDLPLIIKTVGEDNLMVGSDYTHRDPSMELEFRKEL
                     QARADKGDIPQSAVQKILYDNPKTFYAL"
     gene            1561..1845
                     /locus_tag="VE170_18000"
     CDS             1561..1845
                     /locus_tag="VE170_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17393.1"
                     /translation="MIVLSPEGKAQTSAAKIAGIAQFTDLRGKVVGLLDNSKPNADKL
                     AERFAELLKERYGVAGLITRRKLTAQQGAPKQYLDELASQADFVICGLGD"
     gene            1939..2112
                     /locus_tag="VE170_18005"
     CDS             1939..2112
                     /locus_tag="VE170_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17394.1"
                     /translation="MAKSEKEAFGAPDHPVLVVRHPIGTVKIEEVNEKADAAFEQLVN
                     ILLAPEAARARVA"
     gene            2316..3368
                     /locus_tag="VE170_18010"
     CDS             2316..3368
                     /locus_tag="VE170_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17395.1"
                     /translation="MATAALAAERVEIPDDIWEAQAFFEEQGWSDGLPIIPPTEARVA
                     QMLAATKRLADEVIGAVPPRWAQATVEKIAINGVMAGCKPEYMPTLIAAVEAITDPKL
                     NLYALQATTGGPAVMIIINGPVRHQLNVNGGSNALGEGWRANATIGRFVQLVKRNIGG
                     SYPGTTCKATLGWPGKYSICVGENEEASPWEPLHVERGFKAEQSTVTAISADASIRAS
                     DLDSSKAEGVLINFAQKMEGPSGPEAIMVVCPEHAKIIADDGFSKKDVQKFVWERAAY
                     RMKDLPDETFFQRVKRRSDLKLTRDSIIPVTDKPEDILVIVAGGDGSQSQYIHVWGQS
                     TPEGGSTRSVTKLIKD"
BASE COUNT          845 a          983 c         1076 g          759 t
ORIGIN      
        1 acgaagatct gccgtacatc ctgcaatatg tcggcgagga caatctgatc gtcggctccg
       61 actataccca tcgcgacccg tcgatggagc tggagtttcg cactttattg caacaacgag
      121 ccgaccgggg cgagatccca aagacggcgg tggagaagat cctctacgac aaccccaaag
      181 cgttttatgg gttgtagcag actgctgaga aaagctggag catgcttcga caggctcagc
      241 atgaacggat ttatttcaac gatttcaaat tgatccgttc gtcctgagct cgtcgaagga
      301 ctccgagtga gctttgaagg tgccaagatc acagctcttt aaaaaaccgc cgatccgtat
      361 taagcattga agtaattaat tcactcgtaa ggagatgcca ccgtgcgcat aattgacgct
      421 gacacacata tcgacgaaac cgatgccact tgggagttca tgagcggagg cgatctggcc
      481 tacaagccga cgaccgcgtt tccatccaat cccgatccga aactgccgcc ggcccgttat
      541 tgggtgatcg agggcaagcg gcaggtgcgc ttcatccgca gtgacaagga ttcgggcacc
      601 accgttgaga cccgcgagct gctcgacata gatgcccggc tcaaacatat ggacgagctt
      661 ggcaccgata ttcaggtaat ttatccgaca ttgtttttaa tggaagcgtc ggagaatccc
      721 gaggcgagca cggcgacgcg gaaaagttac aatcgctggt tgggtgagcg ttgcagtcat
      781 tcgggcggcc gcttgcgctg gatatgcatg ccgccagtgc agaaaatcga ttctgtgctg
      841 gaagagctta cctgggcgaa agagcatggc gcttgcggcg tcgtgaaaaa aggcgaccgc
      901 gaagcgggaa agtatcccgc cgatccctat ttcgagccgc tttatgcggc agcggaaaaa
      961 ctcgatctgc cgatctgcat tcatacgggt tccggcattc cggatttttc accggcgcgg
     1021 gagttctccc atggccagtt tatgcgcacc aagggggcgg cgatcaacgg tgtgctcgga
     1081 ttggttttgc acaagatccc gatgaagttc cccaagctgc gcatcggctg catcgaagcg
     1141 gggtctatgt ggaccccgtt tgtggcctac gatttgaaac ggcaggcaat caagcagcag
     1201 gggcgcgagt cggtgggcgt tctcggcgtg ccgctgatcg atattccgac caatgtcttc
     1261 aaggacaacc ggatctgggt cacctgccag gtggacgagg acctgccgct tattatcaag
     1321 accgtcggcg aggataatct catggtcggc tcggactata cccatcgcga tccgtcgatg
     1381 gagctggagt ttcgcaagga gttgcaagcc cgcgccgaca agggcgacat tccgcagtcg
     1441 gcggttcaga aaattctcta cgacaacccg aaaacgtttt acgcgctcta ggagtcgctt
     1501 tgacacagtg agttgcggtt gatgtaaatg gcggcaaatt taataggagg gggacattaa
     1561 atgattgttc tcagcccgga agggaaagcg cagacctcgg cggcgaaaat cgccggtata
     1621 gcccagttca ccgatctgcg cggcaaagtg gtggggctgc tcgataacag caaacccaac
     1681 gccgacaagc tggcggagcg tttcgcggaa ttgttgaaag aaagatacgg cgtcgccggt
     1741 ctgatcacgc ggcgcaagct gaccgcccag caaggagcgc ccaaacagta tctcgacgaa
     1801 ctcgcgtcgc aagccgactt cgtcatttgc ggcctcggtg attgaggctc ctgcacgtcg
     1861 tggagtgtcc acgactcact cgagatccaa aagcgcggac ggttcgcgat cacactgatc
     1921 acccacgcct tcatcgcatt ggcaaagtcc gaaaaggaag cgttcggcgc tccggatcat
     1981 ccggtgttgg tggtgcggca tcccatcggc acggtcaaga tcgaagaagt gaatgagaag
     2041 gccgacgcgg catttgaaca actggtgaat attctgcttg cgcccgaggc ggcgcgggcg
     2101 agggtcgcct agcaaagata gtttcgggtt tcgagtcttg aattgaatca aagattcgaa
     2161 acccgaactt ttttcacgta tttgttcctg agtccgtcac accggcgaaa gccgggttta
     2221 ttttggtctt cggttcgccc ggcagcaaac cgggattgcc gcctgcgcgg gatgacgaat
     2281 atgaatattg agtctattct tgaagggtga aatttatggc aacggcagca ttggcagcgg
     2341 aacgagtcga aataccggat gacatttggg aggcgcaagc cttcttcgaa gaacagggct
     2401 ggtccgatgg cctgccgatt atcccgccca ccgaagcgcg cgtcgcgcaa atgctggcgg
     2461 cgaccaagcg cctggcggat gaagtgatcg gcgcagtacc gccgcgatgg gcgcaagcga
     2521 cggtggaaaa aattgccatc aatggcgtca tggccggctg caagcctgag tatatgccga
     2581 cattgatcgc cgcggtggaa gcgatcaccg accccaagtt gaatctttat gccctgcagg
     2641 ccaccaccgg cgggccggcg gtgatgatca tcatcaacgg cccggtgcgt catcaactga
     2701 acgtcaacgg cggttcgaat gccttgggtg aaggctggcg cgccaacgcc accatcggcc
     2761 gcttcgtgca gctggtgaag cgcaacatcg gcggttctta tccaggcacg acttgcaaag
     2821 cgaccttggg ctggccggga aagtattcca tctgcgtcgg tgaaaacgag gaagcgagtc
     2881 cctgggagcc gctccatgtc gagcgcggtt tcaaggccga gcaaagcacg gtgacggcga
     2941 ttagcgccga tgcgtcgatc cgcgcgtcgg acctggacag ctcgaaagcc gaaggggtgt
     3001 tgatcaattt cgcgcaaaaa atggaaggac cgtcaggtcc tgaagcgatc atggtcgtct
     3061 gccccgagca tgccaagatc atcgccgatg acggcttcag caaaaaggac gtacagaagt
     3121 tcgtctggga gcgtgctgct tatcggatga aagacttgcc cgacgaaacc ttcttccagc
     3181 gagtcaagcg ccgctccgat ttaaagttga cccgcgacag cattatcccg gtgaccgaca
     3241 agcccgaaga cattctggtc atcgtcgccg gcggcgatgg ctcgcaatcg cagtacattc
     3301 acgtctgggg ccagagcacg ccggaaggcg gttcgacgcg gtcggtgacc aagttaatca
     3361 aagattaagc gtttaaaacg ttcaagccgt tcaaatcgtt caatacgttt ccgactgatg
     3421 taactctctt accctcgccc attccgatgg gagaggggag ggtgagggtc tgatttctat
     3481 gtccgttcgc cgaatcacac tctttgctgt tcttttgctt attcttactt tgacagtgca
     3541 gtcatccttc gccgccgaca agctgcgcat cggttactcc ggcgctacca tcaccaactc
     3601 catgttgtgg gtgactcacg aaggccggat gttcgaaaag aacggcatcg acgccgaagt
     3661 cct
//
LOCUS       DASQKS010000695         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_824248, whole genome shotgun sequence.
ACCESSION   DASQKS010000695 DASQKS010000000
VERSION     DASQKS010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824248"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VE170_18015"
     CDS             <1..243
                     /locus_tag="VE170_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ17396.1"
                     /translation="WRHDRGEDIRQLSYMTKIQCAEMASRVVDRSIQVHGGLGLMREL
                     PLEWWYRQVRSLRVTEGTPEVLRWRLAEHIIRNHKG"
     gene            362..1570
                     /locus_tag="VE170_18020"
     CDS             362..1570
                     /locus_tag="VE170_18020"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ17397.1"
                     /translation="MIEITNATPLLSGYRVLDLSSSMGSFCGKVLHDLGMEVIKIEPP
                     GGDAGRLEPPFAKGHAHREGSLRFAYLNAGKKSITLDIATDTGRKIFLDLVAKSDVVL
                     ETFAPGDLAARGLGYAQLVEKHSKLILVSLTGFGQDGPYAHYQAPDIVGNAMGALLYI
                     SGDPKMTPCNPPETQSYYYASLMACYGTMLALWQREMRGIGAWVDASIQASMALHEHV
                     AFNFSSDGRVMKRAGSQHQHNAPANLFQCKNGWIALFVTQTHWPLLLKVWDHHEPELD
                     DPKWINSNLRRQQADYINAQVTSFTMRYTKEDLAELMQKHGIPGLPVNSPSDFMKDPH
                     IQARRFFSQVSHPVLGTFDAPGNFFTLDGKRGGPAPAPLTGQHNHDIFSGELGLSAND
                     LAVLAAERVI"
     gene            1624..2838
                     /locus_tag="VE170_18025"
     CDS             1624..2838
                     /locus_tag="VE170_18025"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ17398.1"
                     /translation="MATANEILKGVRILAFTTGYAGPYAGRLLANYGAEVIKIESRKG
                     GLDTFRHYGQHKDIDEAPRFIECNLGVRSLAINLKHPTGAKLIRELAGKSDAILENFR
                     PRVLNKLGLGDDELRKVNPKLVVVKLPGFGTEGPKSSYGTWGFNLTAFSGITYLWNHP
                     EQDRPVGSQGVYPDHLGFALGPTMMIAALLHSRRSGKGVTIDLAQIEGTAAVLGTTYL
                     ETSVNGDDPKPRGNHYDLAAPHGVYRCQGEDRWCVISVRDDAEWRSFCRIIDRTELCS
                     DARFITHQLRLHNRAELDAIVKEWTAARPAEEVMTQCQAAGVPASVVQTGADLLRDPQ
                     LRHRNYFSAFPESLIGPFEIPRSGFVFKGVPEDSLELPARLGSDNDQILGELLGYDKA
                     TIEQWRREEVLT"
     gene            2882..3865
                     /locus_tag="VE170_18030"
     CDS             2882..3865
                     /locus_tag="VE170_18030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17399.1"
                     /translation="MLRTISSASLVFLITLQTSSVCAQERVKLRISSATKTLGYGPFW
                     IASRMGFFAKQNLDVDVVVIRASDVGIQALAGGSLEIAGSASDAPIAAIEKGLDLVIV
                     GGLINGLTQSIMAAKKFKSYNDLRGATFGAISLTSGVTFALRQVLKEKGLEYPRDYKL
                     LVIGGTPQTYAALVAGSIDAAALSLPVNYAAEEQGFNEIGRFVEVIPNYQLAALSTKR
                     AWAEKNRPVLVRFMRAMAQTMRWIHQNKEPTADYLAKEMNLKPDHARRGWEFYTSTKT
                     WHPDGDLNMDGLHNVAQIYWEQSQSKGPTPSAAKYVDQSYLREALKDSGTR"
BASE COUNT          897 a         1188 c         1213 g          874 t
ORIGIN      
        1 tggcgccacg accgcggcga agatatccgc cagctttcct acatgacaaa aattcaatgt
       61 gccgagatgg ctagccgcgt ggtcgaccgg tcgattcaag tccacggcgg tttaggactg
      121 atgcgcgaat tgccgcttga atggtggtac cggcaggtgc gcagcctgcg cgtcaccgaa
      181 ggcacgcccg aagtgctgcg ctggcgcctg gcggaacata ttattcgcaa tcacaaggga
      241 tgaagaacgg tcgaggatgt ggagcgagga tggaagataa cgtctttcga ttctctatcc
      301 tctattctcc accgtccgtc ctcgcaatca gaggattcta cgctttgatc gaaccctgca
      361 catgattgaa atcaccaacg caactccgct tctttctggc tatcgcgtcc tagacttgag
      421 ttcgtccatg gggtcgttct gcggcaaggt gctgcacgat ctcggtatgg aagtcatcaa
      481 gatcgagccg ccgggcggcg atgccgggcg gttggagccg cccttcgcca aaggccatgc
      541 gcaccgcgag ggcagcttgc gttttgccta tctcaatgcc ggcaagaaga gtatcaccct
      601 ggatatcgcc actgacactg ggcgaaagat atttctcgat ctggtcgcga aatccgatgt
      661 cgtgcttgaa acattcgcgc cgggcgattt ggcagcgcgc ggtctcggct atgcgcagct
      721 cgtggaaaag cacagcaagt tgatcctggt ttcgcttacc ggttttggcc aagacgggcc
      781 gtacgctcat taccaggcgc cggatatcgt cggcaacgcc atgggggcgt tgctctacat
      841 cagcggcgat cccaagatga cgccgtgcaa tccgccagag acccagtcct attattatgc
      901 aagcttgatg gcttgttatg gcacgatgct ggcgctgtgg cagcgcgaaa tgcgcggcat
      961 cggcgcctgg gtcgatgcct ctatccaggc gagcatggcg ctgcacgagc atgtggcttt
     1021 caatttttct tccgacggcc gggtgatgaa acgcgccggc agccaacatc agcacaacgc
     1081 gccggcgaat ctgtttcagt gcaagaacgg ctggatcgcg ttattcgtca cacagaccca
     1141 ctggccgctg ctactcaagg tgtgggacca tcatgaaccc gaattggacg atcccaagtg
     1201 gatcaatagc aatctgcggc gccagcaagc ggattatatc aacgcgcaag taacttcgtt
     1261 cacgatgcgc tacaccaagg aagatctggc cgagctgatg cagaagcatg gcatcccggg
     1321 actgccggtg aactcgccgt cggacttcat gaaggacccg catatccagg cacgcagatt
     1381 tttcagtcag gtgagtcatc cggtgctcgg cactttcgat gcaccgggta atttctttac
     1441 gctggacggc aaacgcggcg gtcccgctcc cgcgccccta accggacagc acaatcatga
     1501 tatattttcc ggtgagttgg gattaagcgc aaatgatctg gcggtgttgg cggcggaaag
     1561 ggttatttga acgattccca ataccgattg cagaaaatcg ccactttgta atttgaaaaa
     1621 tctatggcaa cggcaaacga aatattgaag ggcgttcgca tcctcgcttt caccaccggt
     1681 tacgccggtc cgtacgccgg caggttgctg gcgaactatg gcgcggaagt gatcaagatc
     1741 gaatcgcgca agggcggact ggacaccttt cgccattatg gccagcacaa ggacatcgat
     1801 gaagcaccgc gctttatcga gtgtaatctg ggggtgcgct cgctggcgat caatcttaaa
     1861 cacccgaccg gcgccaagct gatccgtgaa ctcgccggta aatcggatgc gattttagag
     1921 aattttcgtc ctagagtatt gaataagcta ggtttaggcg atgatgagtt gcgtaaagtt
     1981 aatcctaaac ttgtggttgt gaaactgcct ggttttggta ccgaaggacc caagagcagt
     2041 tatgggactt ggggtttcaa cttgaccgca ttctccggca ttacttattt gtggaatcac
     2101 cccgagcagg accggcccgt cggcagccaa ggcgtctacc cggaccactt aggttttgca
     2161 ttagggccga cgatgatgat cgcggcgctt ttgcacagcc ggcgcagcgg caagggcgtg
     2221 accatcgacc tggcgcagat cgaaggcacg gcggcggtgt tgggcacgac gtacctggag
     2281 acttcagtca acggcgatga tcctaagccc cggggcaacc attatgattt ggcggcgcct
     2341 catggtgtct atcgttgtca aggcgaagat cgctggtgcg tgatttcggt gcgcgacgat
     2401 gccgagtggc gcagcttctg ccgtatcatc gaccgcacgg aactctgcag cgacgcgcgg
     2461 tttattaccc atcaattgcg ccttcataac cgcgcagaac tcgatgctat cgtcaaagaa
     2521 tggactgcgg cgcgcccggc ggaagaggtg atgacccagt gccaagcggc cggcgtgccg
     2581 gccagcgtcg tgcagaccgg cgccgacttg ctcagggatc cgcagctgcg ccatcgcaac
     2641 tacttctcgg cctttcccga gtcgctgatt ggcccatttg aaattccgcg cagcggcttt
     2701 gtcttcaaag gcgtacccga agattcgctc gagctgccgg cccggctcgg ctcggacaac
     2761 gatcaaatcc tcggtgaact tctgggctac gataaagcca ccatcgagca atggcgccgg
     2821 gaagaagtat tgacctaacc ctttcaagag tccggcggcc ggccggtggg gaggagaatt
     2881 catgctgcga acaatctctt ccgcctcatt agtctttctc attacgcttc agacaagttc
     2941 ggtgtgtgca caggagcgtg tcaagctacg catcagctcg gcgaccaaaa cccttggcta
     3001 cggtccattt tggatcgcct cgcgcatggg ctttttcgcc aaacaaaacc ttgacgtcga
     3061 cgtcgtggtg attcgcgctt ccgatgtcgg cattcaggcg ctggccggag gttcgttgga
     3121 gatcgccggc agcgcgtcgg atgcgcccat cgcggccatc gagaaagggc tcgatctggt
     3181 gatcgtcggc ggtctcatca acggcctgac gcaatcgatc atggccgcca agaaattcaa
     3241 atcctacaac gatctgcgcg gcgctacttt cggcgccatc agtctcactt ccggcgtcac
     3301 cttcgcgctg cgccaagtgc tgaaggagaa agggctcgaa tacccgcggg attacaaact
     3361 gctggtcatc ggcggtacgc cgcagaccta tgcagcactg gttgccggtt ccatcgatgc
     3421 ggcggcactg tcgctgcccg tgaattatgc ggcagaggag cagggattca acgaaatcgg
     3481 tcgttttgtc gaggtgattc ccaactatca actggctgca ctatcaacca agcgggcatg
     3541 ggcggaaaaa aaccgtccgg tgctcgtccg tttcatgcgc gccatggcgc aaaccatgcg
     3601 ctggattcac cagaataaag agccaacggc ggactacctc gccaaggaaa tgaacctcaa
     3661 gcccgaccac gcccggcgcg gctgggaatt ctacacgtcg accaagacct ggcatcccga
     3721 cggcgatctg aacatggacg gcctgcataa tgtcgcgcaa atctattggg aacagagcca
     3781 gtccaaaggc ccgacaccga gcgcggcgaa atacgtcgat cagagttatc tgcgcgaggc
     3841 cctgaaggat tcgggtacgc gctagtagcc tcaccgagca tttcgttgga atcctctcga
     3901 tgcactacgg cgctgcgcaa gcgaccgtct ccctgcaatc gagtacgccg ccgtttgtgc
     3961 gaaaccggac tggtaccggc gacccatgac acgacccggt cgctggcagc gaccagcggt
     4021 gcctgagcga acaggctgcg atttccgcgc tgaatctgcg gcgcgcagaa gagcgtggtc
     4081 tttttaccct tggccgcttc ggtgtggagg tactcgatag gttcgatgat cgcatcgcac
     4141 ctacggtcga acaacgccgc ttcgaactcg cg
//
LOCUS       DASQKS010000696         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_365714, whole genome shotgun sequence.
ACCESSION   DASQKS010000696 DASQKS010000000
VERSION     DASQKS010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365714"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VE170_18035"
     CDS             <1..1127
                     /locus_tag="VE170_18035"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYJ17400.1"
                     /translation="QNLRGQPSKLEVGWRFLKGAKGALDTEKIAAALEKYFYRPLWHD
                     GEPLFMHHLKFKPVDHDPVLTGEEDFNPAKHNWLRSDKVPMLVLNATTVNTGHAWQFT
                     PTWMGESPWSTHPAADSVPRLQWHLYDPESKWQMELARAVAASACVPGLFEPLRIDDA
                     YEGGYQVQLIDGGVHDNQGTIALLAHNCSVLIVSDAAGHLLLEQVPQPGLGGLASYAT
                     RAMDTLMERVRLANYADLAARVRTKLVRGLMFLHMKDGLDADIIRLKFSQDSYDLHRT
                     PLSPLGVRKDFQKAVAELRTDLDAFTDNERNALMACGYRMAATAFERDLRQFDELYVD
                     VLPGKWPFDAMLQEIKSTASSTPKRESLLTALRAGSQRTV"
     gene            complement(1155..1547)
                     /locus_tag="VE170_18040"
     CDS             complement(1155..1547)
                     /locus_tag="VE170_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17401.1"
                     /translation="MGIRRKYKKKQTSFITAVQLDLDTEGFAYNKWGGEQLCKRGDWL
                     VDNDGDKYTVSEEAFARTYEFVSPGVYTKSAPIWAEVADKPGKVKTNEGETAYEAGDY
                     LVSNNQDGTDAYAIGAEKFRQLYDLCED"
     gene            2032..2823
                     /locus_tag="VE170_18045"
     CDS             2032..2823
                     /locus_tag="VE170_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HYJ17402.1"
                     /translation="MAERVWDKFLTEQDKATLVGKADRRIGFGERPALLLIDLYRWVF
                     GDKPEAVTEGIKTWPGSCGLAGWNALPHIQKLLAKCREVGIPIIHVTGMDGVAVEPWA
                     FRRDTKKRATMSAEELDRFRRRYDIVDEVKPIPGETVFKKVSPSAFWGTPLVGHLNFL
                     GVDTIIACGESTSGCVRASVVDGTTNRLRMVVAEECVFDRHEACHAINLFDMNQKYAD
                     VLPLDDIIKYLDGWRAEKAGQVGYANNSMEYLKDLGIGETYKGLR"
BASE COUNT          650 a          834 c          907 g          656 t
ORIGIN      
        1 ctcagaacct gcgcgggcaa ccctccaagc tcgaagtcgg ttggcgattt ctcaaaggcg
       61 caaagggagc cctcgacacg gaaaagatcg cggcggcgct ggagaagtat ttttatcgac
      121 cgctgtggca cgatggcgag ccgctgttca tgcatcatct caaatttaaa cccgtcgatc
      181 acgatccggt tttgaccggc gaggaggatt tcaatccggc gaaacacaac tggctgcgca
      241 gcgacaaagt gccgatgctg gttctcaacg cgaccacggt gaacaccggc catgcgtggc
      301 aatttacgcc aacttggatg ggtgaatcgc cctggtctac tcatcccgcg gcggacagcg
      361 tgccgcgctt gcagtggcac ctgtacgatc ccgaatccaa gtggcaaatg gaactcgcca
      421 gagcagtggc cgcgtcggct tgcgtgccgg gactttttga accgctgcgc attgacgacg
      481 cctacgaagg aggctatcag gtgcagctga tcgacggcgg agttcatgac aatcaaggca
      541 ccatcgcgct cctggcgcat aattgcagcg tgctgatcgt cagcgacgcc gcaggacacc
      601 tgctgctcga acaagtgccg caaccgggac tcggagggtt ggcgagctac gcgacccgcg
      661 ccatggacac gctgatggaa cgggtccggc tggcaaacta tgccgatctt gccgcgcggg
      721 tgcgcaccaa gctggtgcgc ggattgatgt tcctgcacat gaaagacggc ttggatgccg
      781 acatcatcag actaaagttt tcccaggact cctacgattt gcaccgcacc ccgctctcgc
      841 cgctgggcgt gcgcaaggat tttcaaaaag ccgtggcgga actgcgcacc gatctggacg
      901 ctttcaccga taacgagaga aacgcgctga tggcttgcgg ctatcgtatg gcggcgaccg
      961 cattcgagcg cgacctgagg caattcgacg agttgtatgt cgatgtgttg ccgggaaaat
     1021 ggccctttga cgccatgctg caggagatca aatcaactgc gtcgtccacc cccaagcgtg
     1081 aaagtctact caccgcccta cgcgccggaa gccagcgaac ggtgtgagcg cggcacgcag
     1141 tgcagcctct cgcttcaatc ttcgcagagg tcgtaaagct ggcgaaactt ctcggcgccg
     1201 atggcgtagg cgtcggtgcc gtcttggttg ttggagacta gataatcgcc ggcctcgtag
     1261 gccgtttcac cttcgttggt tttcactttt cccggtttgt ccgcaacctc ggcccagatc
     1321 ggcgccgatt tcgtatagac gcctgggctg acgaattcgt acgtcctggc gaacgcctct
     1381 tcagataccg tgtacttgtc gccgtcattg tcgaccagcc agtcgccgcg tttgcagagt
     1441 tgctcgccgc cccatttgtt gtaagcgaaa ccttcggtgt cgaggtcgag ctgaaccgca
     1501 gtgatgaagg aggtctgctt ctttttgtat ttgcgtctga tacccattgc ctcgccttta
     1561 tctaaagttc gtcagccagt ttgctgtgga taatgtagca cgttcaggcg agattgttca
     1621 gcgcgaagag ctagcgggca cagtcgcgac caaatgggca agaattttca gcacggcgga
     1681 cacgaagggc acgcagttta gagtttggat tacccaaact tcctcgtcct ccttcgaaaa
     1741 tacacatgtg gcgatccggg cgatgaagag gcgcccggga tccgtcatac ccgcgaaagc
     1801 gggtatccag gctaattggg cgcagatcaa acctggattc ccccgtgcgc gggaatgacg
     1861 agtcgaaaaa aagatgagta tgtggagggg aatccctccg ttttcacgtt gtaggagtgc
     1921 gtaagatcat gattcacttc gtggtgaatg caatttttct cctccgggcg atgttgacga
     1981 tcgccgcggc gcggtgataa gtcttggctc aattctatat ggaggtgctg catggctgag
     2041 cgtgtttggg acaagtttct cacggagcaa gataaggcga ctttggttgg caaggcggat
     2101 cggcgcatcg gcttcggcga gcggccggcg ttgctgttga tcgatttata tcgctgggtg
     2161 tttggcgata aaccggaggc ggtgactgag ggaatcaaaa cttggccggg aagttgcggt
     2221 ctcgcgggtt ggaacgcttt gccgcatatt caaaaactgc tcgccaagtg ccgcgaagtg
     2281 ggcattccga ttatccacgt gaccggaatg gacggcgtgg cagtggagcc atgggctttt
     2341 cgccgcgaca ccaagaagcg cgcgaccatg agcgccgagg aactggaccg ttttcgccgc
     2401 cgctatgaca tcgtcgacga ggtgaaaccg atcccgggtg aaaccgtgtt caagaaagtc
     2461 tcgccgagcg ctttctgggg cacgccgctg gtcggccacc taaactttct cggcgtcgat
     2521 accatcattg cctgcggcga gagcaccagc ggttgcgtgc gcgcaagtgt cgtcgacggc
     2581 acgaccaatc ggctgcgcat ggtcgtcgct gaagaatgcg tgttcgaccg ccatgaagcc
     2641 tgccatgcga tcaacctgtt cgacatgaat cagaaatacg ccgatgtgct gccgctcgac
     2701 gatatcatca agtatctcga cggttggcgc gctgagaaag ccggccaggt cggctacgcc
     2761 aacaacagca tggagtatct gaaagatctc ggtatcggcg aaacctataa ggggttacgt
     2821 tagccgcgtt attctgttca agggttcaag gttcaaaagt tcaaggttcg gacggggagc
     2881 gaaacgttga gcttttaacc ttgaaccgtt gaaccaatga tttgtttttt cagccccagc
     2941 cagacgacga ccagtatcac cgccgatatc gcggccatgc cgtgatagcc ggtctcatag
     3001 ccaaagtgtt gcaccagtag gccggccatc aatggcccca aggtatt
//
LOCUS       DASQKS010000697         6852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5449753, whole genome shotgun sequence.
ACCESSION   DASQKS010000697 DASQKS010000000
VERSION     DASQKS010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5449753"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1035
                     /locus_tag="VE170_18050"
     CDS             94..1035
                     /locus_tag="VE170_18050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYJ17403.1"
                     /translation="MKVLKLSAAAAVTAFSLITYLYLFDFPRFSLIYGCWKNATDLSA
                     CYLGRSHDFTVELNGHVYEGNTGNLIDKSILSYGAFEKPVLFFLRDVMTSAYSNQGVF
                     LDIGANTGQHTLFMSRHAKEIHSFEPWEPVLKRLRRHIEINRIKNVVIYPFGLGNENS
                     KQPFYAPPADNLGMGSFVKEFASPNPPEGVLEIRVGDEVLEKAGVTSVALIKMDIEGY
                     EKLALKGLKRTLRRHRPIVEFELTADPKSPVSIKSYDELQSLFPENYEFLTFTEGGDS
                     LTGAYVLAPIAELLRYDKSEQHDIVAYPIEKKTFIVR"
     gene            1478..1606
                     /locus_tag="VE170_18055"
     CDS             1478..1606
                     /locus_tag="VE170_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17404.1"
                     /translation="MHNTSIGKYDKTRIPDELDDRTLCRVKHFDKATTLRRSNGTL"
     gene            1593..3023
                     /gene="sthA"
                     /locus_tag="VE170_18060"
     CDS             1593..3023
                     /gene="sthA"
                     /locus_tag="VE170_18060"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HYJ17405.1"
                     /translation="MAHYDMLVIGSGPAGQKAAIQAAKVGKKVAIVERKKVVGGICIN
                     SGTIPSKSLREAVLFLSGFRQRNLYGASYRVKRDITFEDLAQRCDHVVKAEQEVIQNQ
                     LIRNSVDFIVGAASFLDPHRIQIRQDCETKEHTADYVVIGVGTVPARPADIPFDGDSI
                     IDSDGLLLLKRLPKSLTIVGAGVIGCEYACILATLGIPVVLVEKRPRLLEFVDSEIIE
                     SLQYQMRNIGITLRLNEEVVGVTKASDNVIVLRLKSGKEIRSPLLVYSAGRIGATSNL
                     GLEKIGLQADDRGRLKVNENFQTALSHIYAVGDVVGFPALASTSMQQGRHAACHAFSL
                     GCDTSTHLLPYGIYAIPEISMVGRNEEELTRDGVSYEIGVARYREIARGQIIGDTVGM
                     LKLLVHTETRELLGIHVIGEGATELIHIGQAVMALGGKLDYFVDTVFNYPTLAECYKV
                     AALAAMNKLPANGACTTSRVSMPLAS"
     gene            complement(3105..3503)
                     /locus_tag="VE170_18065"
     CDS             complement(3105..3503)
                     /locus_tag="VE170_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HYJ17406.1"
                     /translation="MATTIPEPFKELLNKVAYAHLATVMADGSPQVTPVWYSFDGTHF
                     WVNSAKGRLKDKNMRRNKRVALSIQDPDDAYRYLAIQGDVEEITESGADAHIDALAKR
                     YLGKDSYPFRAPGEVRVIYKIRPRKISTMG"
     gene            complement(3534..4181)
                     /locus_tag="VE170_18070"
     CDS             complement(3534..4181)
                     /locus_tag="VE170_18070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYJ17407.1"
                     /translation="MKKSLELVMFDLDGTLADTGHDLVDSVNFTRGHFTLPPLPAPAV
                     HANVGRGVEHLLKQSLPEMTEANFSTVLQVFLARYENHLLDRTVLYPGAVEVLRYFRG
                     KRRAVVSNKIHRLTLAVVQGLGVAAEFDAILGGDSSADKKPHPAMLQSVLQRYHIDPA
                     NALIVGDGDTDIEAGKRAAVITCGVTYGLGDKDAMRAAGPDLTIDSLIELADFFC"
     gene            4301..5296
                     /locus_tag="VE170_18075"
     CDS             4301..5296
                     /locus_tag="VE170_18075"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17408.1"
                     /translation="MALIRCTEALASLALLLLVTANFADAQKLNTAYISTTPGTSTVI
                     QVAKEARLFDKHGIDATVIFISGSVRGIQAILAGEIPIGEGGGPGLASARLAGGDVVA
                     IAGNVNVLPYYLVSHPSIKRHEDLRGKIGGTHIAGTTAEFALKVGLKRIGLDPLKDVS
                     LRVIGGAIERMVALQKGIVQFTVVTEAGKSQAERLGYPTVVDMVTLQIPFPQNGIYTS
                     TKLIRENPDLVRRYMRAYVEALHFYKTQKEDTLRFMRKYSRIEDRRMLDEAWDWHAKF
                     MPDAPYPPIDGYQLVLQDLAERNSKAAQANVKDYIDARFVKELEDSGFIKGLYRR"
     gene            complement(5381..5836)
                     /gene="grdA"
                     /locus_tag="VE170_18080"
     CDS             complement(5381..5836)
                     /gene="grdA"
                     /locus_tag="VE170_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_081427418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5711..5713),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HYJ17409.1"
                     /translation="MQKGKKIIAIGERDGVTGPVIAECLEGAGYEVVFQATECFVUTA
                     AGAMNFENQGVIKRLADQYGPENLTVVLGNGEANGVEVFAETVTEGDPSFAGPLAGVA
                     LKIPVYHMLEEDVMGQVPEKLREEKLQLSALVIDIEPMHQVLEASRAKL"
     gene            complement(5848..>6852)
                     /gene="grdC"
                     /locus_tag="VE170_18085"
     CDS             complement(5848..>6852)
                     /gene="grdC"
                     /locus_tag="VE170_18085"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HYJ17410.1"
                     /translation="PPAELQNLCDKGALPLYSGAQLVGAFEAAHEEDASLAAEVLLEN
                     LSGKVSAAHALRLVLAQLALKPDDVDFVISCSEEAVGDRYNRGGGNLAKAIAEDVGCG
                     NASGCDVKAFCAGPMYAMVQGASMVQAGVAKRVAVVAGGSLAKLGMKFESHVKKNMPV
                     LEDMLGSFAAILSAAKPGQVCMRLDSAAFHPVRAGGSSQAMAEELAEKPLAKLGMKLT
                     DVDRYGVELHNPEITVPSGSGDVPRTNYLTLAALAVMRGQIQKNQMEDFVRSRGMLGY
                     APTQGHIPSAIVYLGHAKAALESGAIRNALLMAKGSLFLGRMTQLSDGCSFLVDRNEV
                     "
BASE COUNT         1564 a         2027 c         1801 g         1460 t
ORIGIN      
        1 aaatcagtcg cacatcgcaa ccgcgtcata gacaaaacct aagggtgccc tgcatcacgg
       61 caatcgaaca ccgacgggaa aaaaggtgaa tatttgaaag ttctcaaatt atcagcggcg
      121 gccgcggtta ccgcttttag tcttattact tatctatatt tattcgattt tccccggttc
      181 tctcttatct acgggtgctg gaaaaatgcg acggacctat cagcttgcta cctcgggaga
      241 agtcatgatt tcaccgttga gctcaacggt cacgtctacg aaggtaacac cggaaatttg
      301 atcgacaaga gcattttatc ctatggggct tttgaaaagc ctgtattatt tttcctgcgg
      361 gatgttatga catcggcgta ttctaaccaa ggcgtattcc tcgatatcgg cgctaacaca
      421 gggcaacata ctctttttat gtctcgccat gccaaggaaa ttcattcctt cgagccctgg
      481 gagccagtgt taaaaagact acgccggcat atcgaaatca atcgcatcaa gaacgttgtc
      541 atatatccgt tcggactggg aaatgagaac tcaaagcagc cattttatgc gccccccgcc
      601 gacaatttag gaatgggctc attcgtaaaa gaattcgcat cgccaaaccc gccggaaggg
      661 gttcttgaaa ttcgcgtggg tgacgaggtg cttgaaaagg cgggtgtcac gtcagtagcc
      721 ctgatcaaaa tggatattga gggctacgaa aagctggcac taaaagggct aaagcgcacc
      781 ctacgcaggc atcggcccat tgtcgaattt gagctgaccg cagatcctaa gagtccggtt
      841 agcattaaga gctacgacga actgcaatct ttgtttccag agaattatga attcctgaca
      901 ttcaccgagg gcggcgatag cctgactggc gcgtatgttt tggcgcctat cgctgaatta
      961 ctgcggtatg acaagtctga gcaacatgac atcgtcgcct acccgatcga aaagaaaaca
     1021 ttcatagtgc gctagctgac cccaccttcc ctcctcgtcg ctgaggccga ctccggccaa
     1081 ggaccgccgc gcaacggaaa cttaacttga cgatttctcg cccatcgcat tatgtctccg
     1141 gaagttaata aagccatgct tttcgccaaa aaacacaggc accatcgatc cgattctctc
     1201 acggataaaa gatcgatgtc atcgggacac cttgaaagca ttgcctctgg gcggggcgtt
     1261 gagctttgcc cggcgccacc gtcagaaaat tgacggggca gctgagtttt cagcccgact
     1321 gctgagcgtc acgcggtatt tgccttcgag ctggccgcgt tggaaattcc gctcgacgct
     1381 accgaaagcg ctgaagaagc gctggaaagt gccgatatgc tgattacggc gataaatgca
     1441 ccgacggcgg tcttgaaagc aacagtttga aggaaccatg cataatacct ccattggcaa
     1501 atacgataag acgcgcatcc ccgacgaact cgacgaccgg accctgtgcc gggtcaaaca
     1561 cttcgacaag gcgacaacgt tgaggcgatc caatggcaca ttatgacatg ctggtgatcg
     1621 gctccggccc ggccgggcag aaggccgcga ttcaggccgc caaggtcggg aaaaaagtcg
     1681 ccatcgtgga acgcaagaaa gttgtcggcg gcatctgcat caactccggc acaatcccga
     1741 gcaaatcctt gcgcgaagcg gtactgttcc tttccggatt cagacagcgc aacctttacg
     1801 gcgccagcta ccgggtcaaa agagatatta ctttcgagga cctcgcccaa cgctgcgacc
     1861 atgtcgtcaa agccgagcaa gaagtgattc aaaatcaatt gatccgcaac tcggtggatt
     1921 ttattgtcgg cgccgcctcg tttctcgatc cgcaccggat ccagatcaga caggactgcg
     1981 aaactaaaga acacacagcg gactacgtgg tcatcggtgt cggcactgtg ccagcgcggc
     2041 ccgcggatat ccccttcgac ggcgactcca tcatcgacag cgatggtctg ttattgttga
     2101 agcgcctgcc aaagtcattg accatagtcg gcgccggcgt gatcggctgc gagtacgcct
     2161 gcatcctcgc gactctgggg attcccgtgg tgctggtcga gaaacggccg cgactgctgg
     2221 aattcgtcga cagcgagatt atcgaatcgc tgcaatatca gatgcgcaac atcggcataa
     2281 cactgcggct caatgaagaa gtggtcggtg tgaccaaagc gagcgacaac gtcatcgtgc
     2341 tgcgattgaa gagcggcaaa gagatccgct cccctttact ggtttattca gcgggacgca
     2401 tcggcgccac cagcaacctc ggcttggaaa agatcggttt gcaagcggat gaccgcggcc
     2461 gcttgaaagt caacgagaat tttcagacag ccctgagtca catctatgca gtgggcgatg
     2521 tcgtgggctt tccagccttg gcgtcaacct cgatgcagca aggccgccat gctgcctgcc
     2581 acgcgttcag cctcggctgt gacacgtcga cccatttgct cccctatggc atctacgcaa
     2641 ttccagagat ctcaatggtc ggacgcaacg aagaggaact cacccgcgac ggcgtgtcct
     2701 atgagatcgg cgtcgcccgc taccgcgaga tcgcccgcgg ccagatcatc ggcgacactg
     2761 tcggcatgct caagctgctg gttcataccg agaccaggga gctcctaggc atccatgtaa
     2821 tcggcgaagg cgccaccgaa ctgatccata tcggccaagc cgtcatggcc ctgggcggca
     2881 aactcgatta tttcgtcgac acggttttca actacccgac cttagcggag tgctacaaag
     2941 tcgccgcgct ggcggcgatg aataagcttc cggccaacgg cgcatgcaca acctcgcgcg
     3001 tctccatgcc gctggcttcc tgacatcaat tatcctcatc gccatcctca tggcggagcg
     3061 gcaaaagcgc tgcgaaccag ccattttcaa cgggcaatgt aaattcatcc catcgtcgag
     3121 attttccgcg gacggatctt ataaatcacc cgcacttcgc caggagcgcg aaacggatag
     3181 ctgtctttgc ccaggtaacg ttttgccagg gcatcgatat gggcgtcggc gccgctctcg
     3241 gtgatctctt cgacgtcgcc ttgaatcgcc aggtaacgat acgcatcgtc aggatcttgg
     3301 atcgaaagcg cgactcgctt gttgcgccgc atgttcttgt ccttgagccg gcccttcgcc
     3361 gagttgaccc aaaaatgcgt gccgtcgaag ctgtaccaca ccggcgttac ctgcgggctg
     3421 ccgtcggcca tcaccgtagc caagtgagcg taggcgactt tgttaagaag ctctttgaaa
     3481 ggctccggaa tcgtcgtggc catgattaac ctcctgcttt gtgtataaac aattcaacaa
     3541 aaaaaatcgg ccaattcaat gagactgtcg atggtgagat ccggtcctgc ggcgcgcatc
     3601 gcatccttgt cgcccaatcc gtaagtcacg ccgcaggtga tcaccgcagc gcgcttgccg
     3661 gcttcgatat cggtatcgcc atcgccgacg atgagcgcgt tggcgggatc gatgtgatac
     3721 cgctgcaaaa ccgactgcaa cattgccgga tggggttttt tatccgcgct actatcgccg
     3781 cccaagatcg catcgaactc cgctgccacg cctaacccct gcaccacggc cagcgtaagc
     3841 cggtgtattt tgttgctcac cacggcgcgc cgcttaccgc gaaagtagcg taaaacttcc
     3901 accgctccgg gataaagcac ggtgcgatcg agcaggtggt tttcataccg tgcgagaaac
     3961 acctgcaaga ctgttgaaaa gttcgcctcc gtcatttcgg gcaaagactg ctttagcaaa
     4021 tgctccacgc cgcggccgac gttggcgtgc accgccggcg ccggcaacgg cggcagggta
     4081 aaatgcccac gcgtaaaatt gaccgagtcg accaggtcat ggccggtatc ggcaagggtg
     4141 ccgtcgaggt cgaacatgac tagctctaaa gattttttca ttgcattccg caacctagac
     4201 ttccattgct gccctgtcaa accagctttt cacgcagcgt ttgaaatgtt atgcgtagtt
     4261 gcaccggttc tacccgctaa ccctttctgg aggtattcgc atggctctga tccgctgcac
     4321 cgaagcgctc gcctcattgg cgctgctttt actggtaacg gcgaatttcg ccgatgccca
     4381 gaagctcaac accgcgtata tctcgaccac gccgggaact tcgacggtga ttcaagtcgc
     4441 caaggaagcg cgtctgttcg acaaacatgg catcgacgcc acggtgattt tcatttccgg
     4501 cagcgtgcgc ggtatccagg cgattctcgc cggcgaaatt cccatcggcg aaggcggcgg
     4561 cccaggcctg gccagcgcgc ggctcgccgg cggcgacgtc gtcgccatcg ccggcaatgt
     4621 caacgtgctg ccttactatt tagtttccca cccgagcatc aaacggcatg aagatttgcg
     4681 aggtaaaatc ggcggcactc atatcgccgg caccaccgcc gagttcgcct tgaaggtggg
     4741 gctgaaacgc atcggtctcg acccgctcaa ggacgtcagc ttgcgcgtca ttggcggcgc
     4801 catcgagcgc atggtcgcgc tgcaaaaagg catcgtccag ttcaccgtgg ttaccgaagc
     4861 cggcaaatct caggccgaga gactcggcta tccgacggtg gtcgacatgg tcaccctgca
     4921 aatacccttt ccgcagaacg gtatttatac atcgaccaag ctgatccgcg aaaaccccga
     4981 tctggtgcgc cgctacatgc gcgcttacgt cgaggcgctg cacttctata aaactcagaa
     5041 ggaagacacg ttgaggttca tgcgcaaata ctcgcgaatc gaggaccgcc gcatgctcga
     5101 tgaagcgtgg gattggcacg ccaagttcat gcccgacgcg ccttacccgc ccatcgacgg
     5161 ttaccaactg gtgctgcaag atctagccga aaggaattcc aaagccgccc aggccaacgt
     5221 aaaagattac atcgatgcgc gctttgttaa ggaactggaa gactcggggt tcattaaagg
     5281 gctgtaccga aggtaatacc cgttccaagt tcaaaggttc aaggttcaat cggatcgcga
     5341 tctttccttg aacttttgaa cccttgaacc ttgaacagtt ttaaagcttc gcccgcgaag
     5401 cttctaaaac ttggtgcatc ggctcaatat cgatgactaa cgccgagagc tgcaattttt
     5461 cttcacggag cttttcgggc acctggccca tcacatcttc ttcgagcata tgatagacgg
     5521 ggatcttcag cgccacgccg gccagcggcc cggcaaagct cggatcgccc tcggtcaccg
     5581 tctcagcaaa cacttcgacg ccgttagcct caccgttgcc cagcaccaca gtcaaatttt
     5641 ccggaccata ctggtccgcc aggcgcttga tgactccttg gttttcgaag ttcatcgcgc
     5701 ctgcggcggt tcaaacaaag cactctgtgg cctgaaaaac cacctcgtaa cccgcgcctt
     5761 cgagacactc ggcgatgacc gggccggtaa caccgtcccg ttcgccgatg gcgatgatct
     5821 ttttaccctt ttgcatgcgt cctccaacta aacttcgttg cgatcgacta gaaacgagca
     5881 gccatccgac agctgcgtca tgcggccgag aaacaaactg cctttagcca tcagcagcgc
     5941 gttacgaatc gcaccgcttt cgagcgctgc cttggcatgg cccaagtaga cgatagccga
     6001 cggaatatgt ccctgggtcg gcgcatagcc tagcatgccg cggctgcgca cgaaatcttc
     6061 catttggttc ttttgaattt gcccgcgcat caccgccaac gccgccaagg tcaaataatt
     6121 cgtgcgcggc acatcgccgc tcccagaggg gacggtgatc tcggggttat gcagctcgac
     6181 gccatagcga tcgacgtcgg tcaacttcat gccaagcttg gccaatggct tctcggccaa
     6241 ttcctctgcc attgcctggg acgagccgcc ggcgcgcacc ggatgaaacg ccgcggagtc
     6301 gaggcgcatg cagacctgcc ccggtttcgc cgcgctgaga atcgccgcga aactgcccag
     6361 catatcttcg agcaccggca tatttttttt cacatgggac tcgaacttca tgccgagttt
     6421 ggccaacgac ccgccggcga ctactgcgac tcttttcgcc acgccggctt gaaccatgct
     6481 tgccccctgc accatcgcat acatcggccc agcgcaaaac gctttgacat cgcagcccga
     6541 ggcgttaccg cagccgacgt cctctgcgat cgccttggct aggttgccgc cgccgcgatt
     6601 ataacggtcg cccaccgctt cttccgaaca gctaatgaca aaatcgacgt catcgggctt
     6661 gagcgcaagc tgcgctaaca ccaggcgcaa cgcgtgggcc gcggaaactt taccggataa
     6721 attctccagc aagacttcgg cggccagact ggcgtcttct tcatgggccg cctcgaacgc
     6781 gccgacgagt tgagctccag aatagagcgg cagggcgcct ttatcgcaga gattttgcag
     6841 ctcagcgggc gg
//
LOCUS       DASQKS010000698         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_369789, whole genome shotgun sequence.
ACCESSION   DASQKS010000698 DASQKS010000000
VERSION     DASQKS010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369789"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VE170_18090"
     CDS             complement(<1..388)
                     /locus_tag="VE170_18090"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17411.1"
                     /translation="MSEVLATAESDSGTAPRGSANIRHLSRMELEGGGQIVLDGKYAY
                     VGHQHRVQGTTILDIADPRKPKVVSTLMPNHPWSHSHKVRVIGDVMVVNSEFERGPGQ
                     RGDYPDGGFRIYDIKDRTAPKLITFVK"
     gene            complement(460..1449)
                     /locus_tag="VE170_18095"
     CDS             complement(460..1449)
                     /locus_tag="VE170_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HYJ17412.1"
                     /translation="MKNLTLSLACEDYDRMRPLKDGIVKPEGIELNYMVMPVEEIFWR
                     MMKYEEFDVSELSMGAFLTAASRGRRPFVAIPVFPSRTFRHRCIFVNTESGIKSIQDL
                     RGRRMGVPEYSMTAAVWLRGMFEHEYGVPPSAIHWVQAGEEHPGRKDRVDFEMPAGVT
                     MESRPDTTLNIMIENGEVDAMMSPRMPTCFLQGSPKVKRLFPNYRQVEMDYFKTTGLF
                     PIMHVLVIKRTIYEKAPWVAQTLYKAFCESKDICMRDLYDTNILRVALPWTSAEFEDT
                     TELMTTDYWPYGLDPNRNNLETLNGYLFEQGLTKQKLVLDELFARETVEAFKI"
     gene            complement(1446..2114)
                     /locus_tag="VE170_18100"
     CDS             complement(1446..2114)
                     /locus_tag="VE170_18100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HYJ17413.1"
                     /translation="MDRIKLVAELHASRLVAVVRSTSPEDAMALASAAADGGIKFIEI
                     TFSVPGALDVIKELTRRGGIHVGAGTVLASQQAERALGVGAEFIVSPSLELNLVGLCH
                     TANIACFPGAATPTEIIAAQRAHADLVKIFPADLAGGPYFIRQMQGPFPDARFMVSGG
                     VNLKNIQEYVQTGVTGICLGSAYLGGLLAAKGKKLFVKEMQQFVTLVAQAQSKNSKAR
                     KRGK"
     gene            complement(2173..>2568)
                     /locus_tag="VE170_18105"
     CDS             complement(2173..>2568)
                     /locus_tag="VE170_18105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ17414.1"
                     /translation="CARAAQPLLEASHGMIVNVSSISGVLAPSTMSSLAYGTAKAALI
                     YMTKGLAVAMAPKVRVNCVAPAFTDTPWMSQHFGVDYQQVIAKASAGYPLQRIATPED
                     IAGAILGLITGGDFVTGQTLLVDGGLSLS"
BASE COUNT          530 a          776 c          712 g          550 t
ORIGIN      
        1 ttttgacaaa ggtgatgagc ttcggtgccg ttcgatcttt gatgtcgtag atgcgaaagc
       61 cgccatcagg gtaatcgccg cgctgtcctg gaccgcgctc aaactcggaa ttgaccacca
      121 tcacatcgcc aataacccgc actttgtgcg aatgcgacca cgggtggttc ggcataagcg
      181 tgctaacgac ctttggtttg cgcggatcgg caatgtcaag gatcgtcgtt ccctggaccc
      241 ggtgctgatg gccgacatag gcatatttcc cgtcaaggac aatctgcccg ccgccctcga
      301 gctccatccg ggagagatgg cgaatgttcg ccgacccgcg cggcgccgtg ccgctatcgc
      361 tttccgctgt agctaggact tcactcatat tgcgcctccg aataacatta tctgtgtcaa
      421 taatcggcag cgggtaagtt tcaacgaacc gccgctttgc taaatcttaa acgcttccac
      481 cgtctcccgg gcgaacagct catccaaaac aagcttctgc ttggtcagac cctgctcgaa
      541 caaatagcca tttaaggttt cgagattgtt gcggttggga tccaggccgt agggccagta
      601 gtctgtcgtc atcagctcgg tggtgtcttc gaactcggca gaagtccacg gcagcgcgac
      661 gcgcagtatg ttggtgtcgt agagatcgcg catgcagata tctttcgact cgcaaaacgc
      721 tttgtaaagc gtctgggcca cccatggcgc tttttcgtag atcgtccttt tgatcaccag
      781 cacatgcatg atcgggaaca ggccggtggt tttgaagtag tccatctcga cctggcggta
      841 gttgggaaac aggcgcttca ccttcggcga cccttggaga aagcaggtcg gcatgcgcgg
      901 cgacatcatg gcgtcgacct cgccgttttc gatcatgata ttgagcgtcg tgtccgggcg
      961 gctttccatc gtcactccag ccggcatttc gaagtcgacc cggtctttgc ggcccggatg
     1021 ctcctctccc gcctggaccc agtgaatcgc cgacggcggc acgccgtact catgctcgaa
     1081 catgccgcgc aaccacactg ccgcggtcat tgagtactcc ggcacgccca tgcgccggcc
     1141 gcgtaaatct tgaatggact tgatacccga ctccgtgttg acgaatatac agcgatggcg
     1201 gaaggtgcgc gatggaaata ccggaatcgc cacgaagggc cggcggccgc gcgatgccgc
     1261 ggtcaaaaat gcccccatgg acagttcgga aacgtcgaac tcttcatact tcatcatgcg
     1321 ccagaagatt tcttccaccg gcatgaccat atagttgagc tcgatgcctt cgggtttgac
     1381 gatgccgtct ttaagcgggc gcatacggtc gtagtcctcg catgccagcg aaagagtgag
     1441 attcttcatt tgccgcgctt tcttgctttg ctattcttgc tctgcgcctg ggcgaccaag
     1501 gtgacgaatt gctgcatctc tttgacgaac agcttcttgc cctttgccgc cagcaagccg
     1561 ccaagatagg cactaccgag acaaattcca gtgacgccgg tttggacgta ttcttgaatg
     1621 tttttcagat tcacgccgcc cgacaccata aagcgcgcgt ccggaaacgg cccctgcatc
     1681 tgccgaataa aatacggccc gccggctaaa tccgccggga aaattttcac caggtcggca
     1741 tgcgcccgct gcgccgcgat aatctccgtc ggcgtcgccg caccggggaa gcaagcgatg
     1801 tttgccgtat ggcaaagacc caccagattc agctccaacg acggcgagac gatgaactcg
     1861 gcgccgacgc cgagggcgcg ctccgcctgc tgtgacgcca ggacggttcc ggccccgaca
     1921 tgaataccgc cgcgccgggt tagttctttg attacatcca gagcgcccgg cacgctgaaa
     1981 gtaatctcga taaacttgat cccgccatcg gcagcggcac tggcgagcgc cattgcatcc
     2041 tcggggctgg tcgaacgcac gacggcgact aagcggctgg cgtgtagttc tgcaactaat
     2101 tttattctgt ccataatggt tggtcgtgcg atccgctgaa tggcgagaat ctcctacctg
     2161 ttgactggaa tttcaactga ggctcaatcc gccatcgacc aacagagtct gcccagtcac
     2221 gaagtcgcca ccggtaatca aaccgagaat cgcgccggcg atgtcctccg gtgtagcgat
     2281 gcgttgcagg ggataacccg cggacgcttt ggcaatcacc tgctggtaat cgacgccaaa
     2341 gtgctggctc atccacggcg tatcggtaaa tgccggcgcg acgcagttga cgcgcacctt
     2401 gggcgccatc gccacggcca agcccttggt catgtaaatc aacgccgcct tcgccgtgcc
     2461 gtaggccagc gacgacatcg tcgacggcgc cagcacaccg gagatcgacg atacattgac
     2521 gatcatcccg tggctggctt caagcagcgg ctgcgcggca cgggcaca
//
LOCUS       DASQKS010000699         5681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3351837, whole genome shotgun sequence.
ACCESSION   DASQKS010000699 DASQKS010000000
VERSION     DASQKS010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3351837"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VE170_18110"
     CDS             complement(<1..631)
                     /locus_tag="VE170_18110"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17415.1"
                     /translation="MERNAARAILVFSLLLLWCNHGDSQTPRKLESVTLGLSARHVLN
                     LPIYMAQRHGIFESEGFDYKPITTKTNTAIAALVSGDLDYITAFNSGLGAAIGGAPLV
                     AVYVSTDRPLLYLISRPNIKDAKSLRGGVIGHGGTRGSHYHATVTMVKHLGLDAEKDV
                     QLISTLDVTQGMTALFSGNVAATTLSPPYDSIAVRRGYNRLVMGPEILPR"
     gene            complement(705..1697)
                     /locus_tag="VE170_18115"
     CDS             complement(705..1697)
                     /locus_tag="VE170_18115"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17416.1"
                     /translation="MNKWRKIGAAAIGLSGLFFTASAHSQELRKVRVGIPTLSPIIAA
                     TLVPRDKGYFKQEGLDVETIVIRSAPSVLALAAKEIDFLMTGGGGLSGVLRGLPLRVV
                     FAPLRRPTYALYAKAEIRTIAELEGKRIGISSYGSGPDLLLRDVLKKRLADGGKKVTI
                     LAVGSGTERYIALKTNVVDAAVLSSPVTLTAKQEGYRELFSFFTDKEYADIPVATFSR
                     EDMLQSNTSLVERFVRAQVKGLLYMRANRDGAASALARALKSKESETGPAFDEIRPTM
                     TEDGTISLDEQRKALEYLLAPAQQKDPPRLERIYDFALARKAYQELQGRGWKPE"
     gene            complement(1765..2937)
                     /locus_tag="VE170_18120"
     CDS             complement(1765..2937)
                     /locus_tag="VE170_18120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ17417.1"
                     /translation="MPQSESAPKETSAHPWLKDFVLTWLSRFGIFLAHHVTRPLLPLY
                     LITFGASSTVIGAVMAVFTITATVMRIPVGLLIDRVGRKPFLLYGIVLFTAGKLGYLW
                     APSILLMLPFRVLHGLGWSGCTTAVSTINADIVPQARRGELMGYAGMASSLASALGPV
                     LGFALFHRFDYSGVFIGACALLIVSFIFAVPVAEPKRPATEPYKAERWIDNIMVRESL
                     DPAIGVAFLSFGHGGILTFLPIHALKLGMANPGIWFGVYAACILLSRPIAGPVSDRVS
                     RRAVIIPGLILNLAGIAMLAFATSSYGLMAAAIVGGFGTGAAQPALMTQAVDRSDGQR
                     RGQSLAQFQFFYDLGIGIGSLTLGALLDLVNQSFRIMYLATAVVALIGLWINWRQK"
     gene            3235..4245
                     /locus_tag="VE170_18125"
     CDS             3235..4245
                     /locus_tag="VE170_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HYJ17418.1"
                     /translation="MGQNYEQVSLEERCTLCRLSQAGKTIRQIAAAMDRAPSTIMREL
                     KRNTGSQVGYQPAYADKQAKSRRWKGSRLLRDPDLQAKVLNRLRQGWSPAQVAGRLNK
                     EYRRQVISHESIYRFIDSQIRRTKDYDWRHYLPRGKSKRGWRGRKGGSSVDRIEGRVS
                     IDQRPAYIDKRRQPGHWEADLMLFAKYGQALLMTHERHSRLLCVSRQPSKAAEPVVKK
                     LTKLFRTLPLGMRRTITFDNGTEFAYHHRLHKLSMRTFFCDAHAPWQKGGIENAIGRM
                     RRGLPRKTDLATLSDHRLLSVVRSYNHTPRKCLNFYTPAEVLTRDLLHFKCESTYPLS
                     RV"
     gene            4427..5584
                     /locus_tag="VE170_18130"
     CDS             4427..5584
                     /locus_tag="VE170_18130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17419.1"
                     /translation="MLQGYRVFDADAHGSINPSMWEDLPEQFRARRPRPVTIHDENGL
                     GNFTAGWLIEGSLEPHALGPGAQQANTPRNVLNEFGANMKREHCSVGSMTLNDPSARL
                     ADMDRMGFDVQMIFPSTLYAHMTSDPEFEAALCRAYNRFVAKQCTADPRRLKWSGLLP
                     LREPRQALLAIEEMQRLGARAAVVFGTAGERLLSDPSFVPIWDEFARTELPLCIHMGM
                     SYPPLAQLGRSIYDGHIIGMSLPAQLAFMAIVGHRMLDRYQDLKVAFLEFGAEWIFYM
                     VSRMDHYLPLDRGAHPFGLSMPNPGDLPRASIRDYVKSGRIFIAAEADDRMLQQLFDL
                     IGEDHVLFSSDFPHGEGRDNAALEILERQDLAEPQKRKLLYANTVRFFGAP"
BASE COUNT         1236 a         1615 c         1672 g         1158 t
ORIGIN      
        1 agcgcggcag gatttccggg cccatgacga ggcggttata acccctgcgc acagcgatgg
       61 aatcgtaggg cggcgacaat gtcgtggcgg cgacattgcc ggaaaagagg gccgtcatac
      121 cctgggttac gtcgagggtc gaaatcaact ggacgtcttt ctcggcatcc aagccgagat
      181 gcttcaccat ggtgactgtg gcgtggtaat ggctgccacg ggtgccgcca tggccgatga
      241 cgccgccgcg cagactcttg gcgtctttaa tattcggccg gctgatcaaa taaagcagcg
      301 gtcggtcggt ggaaacgtag accgccacca aaggtgcgcc gccgatggcc gcccccaagc
      361 ccgagttgaa tgcggtgatg tagtcgagat cgccggagac cagcgcggca atggcggtgt
      421 tggtcttggt cgtgatcggt ttatagtcga agccttcgct ctcgaagatg ccgtggcgct
      481 gggccatgta gatcggcaga ttgagcacat ggcgcgccga caggccgagg gtgacggatt
      541 ccaatttcct tggcgtttga ctgtcgccgt ggttgcacca gagcagcagc agcgaaaaga
      601 cgaggatagc ccgtgccgcg tttcgttcca tagcttgctt caacgaaaac aattgtgccg
      661 gatcatattt tcatcggtgc tttggcacaa gcaaaattgc aatgctattc cggcttccaa
      721 ccgcgcccct ggagttcctg ataggccttt cgcgccagag cgaaatcgta gatgcgttcc
      781 agccgcggcg gatctttttg ctgggccggg gcgagcaaat attccaaggc tttgcgttgc
      841 tcgtcgagac tgatggtgcc gtcctcggtc atggtcgggc ggatctcgtc gaaggccggt
      901 ccggtttcgc tctctttgga tttgagggcg cgagctagcg cgctggcggc gccgtcgcgg
      961 ttggcgcgca tgtaaagcaa gcctttgact tgggcccgga cgaatcgttc gaccagtgag
     1021 gtattgctct gcagcatatc ttcgcggcta aaagtcgcca ccgggatgtc ggcgtattct
     1081 ttgtccgtga aaaatgagaa cagctcacga taaccttcct gtttggcggt caaggtcacc
     1141 ggcgatgaca atactgcggc atcgacgacg ttggtcttga gggcgatgta acgctcggtg
     1201 ccgctgccga ctgcgagtat cgtaactttc ttgccaccgt cggccaggcg cttcttcaat
     1261 acgtctcgca gcagcagatc ggggccgctg ccgtagctcg atataccgat ccgctttcct
     1321 tcgagttcgg cgatggtgcg aatctcggcc ttggcataaa gcgcgtaggt tggccggcgc
     1381 aacggcgcaa atacgacgcg cagcggcaaa ccgcgcaaca cgccgctgag gccgccgccg
     1441 ccggtcatga gaaaatcgat ttccttggcg gcaagagcga gaaccgacgg cgcggagcgg
     1501 atgacgatgg tctcgacatc gagcccttcc tgtttgaaat agcctttgtc tctcggcacc
     1561 agtgtggcgg cgatgatcgg cgacagagtc gggatgccga cgcggacttt gcgcagctct
     1621 tggctgtgcg cggaggcggt gaaaaataac ccgctaagac cgatagctgc tgcgccaatt
     1681 tttcgccatt tgttcataat gccccgacct ctctcttcga aataggcgag ggaacggtaa
     1741 aacgattgct gccgcctcag tgaactattt ttgccgccaa ttgatccata gaccgatcaa
     1801 cgccacgacc gcggtggcga gatacatgat gcgaaaactt tggttgacca aatcaagtaa
     1861 tgcgccgagg gtcaaggagc cgatgccgat gccaagatcg tagaaaaact gaaactgcgc
     1921 cagcgattgg ccgcgccgtt ggccgtcgct gcgatccacc gcctgggtca tgagcgccgg
     1981 ctgtgccgcg ccggtgccga agccgccgac gattgccgca gccatcaagc cataggacga
     2041 tgtcgcaaat gccagcatag cgataccggc gagattcaaa atcaatcccg gaataatcac
     2101 cgcgcggcgg ctgactcgat ccgaaaccgg tccggcgatg ggccggctca aaagaataca
     2161 agcggcgtaa acgccgaacc agatgccagg attggccatg ccgagtttca gagcgtggat
     2221 gggcagaaac gtcaggatgc cgccgtggcc gaaggaaaga aacgccacac cgatggccgg
     2281 atcgagggat tccctgacca tgatgttgtc gatccagcgc tcggctttgt agggttcggt
     2341 tgccggtcgt ttcggctcgg cgacagggac ggcgaagata aaactgacta tcagcaaagc
     2401 acaggcgccg ataaacacgc cggaatagtc gaagcgatga aacagcgcga agcccaatac
     2461 cgggcccagc gcggaagcga ggctgctggc catgccggcg tagcccatca gttcgccgcg
     2521 ccgcgcctgt ggcacgatgt cggcgttgat ggtcgagacc gcggtggtgc agccagacca
     2581 gccgagacca tgcaataccc gaaatggcag catgagcaaa atcgacggcg cccacagata
     2641 gcccaacttg ccggcggtga ataggacgat gccgtagagt aaaaacggtt tgcggccgac
     2701 acggtcgatg agcaaaccga cgggtatacg catcaccgtc gcggtgatcg tgaacaccgc
     2761 catcaccgcg ccgatgacgg tggacgacgc gccgaaagta atcagataaa gcggcagcag
     2821 cggccgggtg acatggtggg cgagaaaaat gccgaagcgg ctaagccagg tgaggacaaa
     2881 gtctttaagc cagggatgcg ccgatgtttc tttgggtgct gattcgctct gcggcatggg
     2941 aagtttcgat cattgcggca ggggcgagac aaagtcaatt gatgttgcga tggcgagcaa
     3001 acggaaatgc tctcttacca cgaaggcacg aagagcacga agttcggaat gttatttttc
     3061 cgaaaccttc gtgtctcctt agaaaataca tgtaagggga gggtcttaat gaagaggtca
     3121 cctgggttcc gtcatacccg cgaaagcggg tatccaggct aattgggcgc agaccaaacc
     3181 tggattccct aaattcacag accaagtgca acacttattg gataaggtgt tgccatggga
     3241 caaaactatg aacaggtttc gcttgaagag cgctgtacgc tttgccgact tagccaagcc
     3301 gggaaaacaa tccgccaaat cgcggcagct atggatcgcg cgccatcgac tataatgcgc
     3361 gagctcaaac gtaacaccgg ctctcaggtt ggctaccaac ctgcctacgc cgacaagcag
     3421 gccaagagcc ggcgctggaa aggtagccga ctattgcgcg atcccgatct tcaggccaag
     3481 gttcttaacc ggctgcgcca aggctggtcg ccggcccagg tggccggtcg gttgaacaaa
     3541 gagtaccgac ggcaggtcat cagccatgag tcgatctatc ggttcatcga ctcccagatc
     3601 cgccgcacca aggactacga ctggcggcac tacttgccgc gggggaaaag caagcgcggt
     3661 tggcgcggtc gcaaaggcgg gagctcggtg gatcgcatcg aggggcgcgt ctccatcgat
     3721 cagcgcccgg cctacatcga caagcgtcga cagccaggtc actgggaagc cgatctgatg
     3781 ctgtttgcca agtatggtca ggctcttctg atgactcatg agagacactc acgcttgctc
     3841 tgcgtcagcc gtcagcctag caaggcggct gaaccggtag tgaaaaaact tactaaactc
     3901 tttcgtacac tgcccctcgg tatgcgtcgt actatcacct tcgataacgg caccgagttc
     3961 gcctatcatc accggctcca taaactatcg atgcgaacct ttttctgtga tgctcatgcc
     4021 ccttggcaga agggcggcat cgaaaacgcc attggtcgca tgcgtcgagg cttgccgaga
     4081 aaaaccgacc tcgctaccct ctccgatcac cgcttgctct ctgtggttcg ctcgtataat
     4141 cacaccccaa gaaagtgtct taacttctat accccggccg aggtcctcac ccgtgacctg
     4201 ttgcacttca aatgtgaatc cacctacccg ctttcgcggg tatgacggag ccccgggggg
     4261 ccttcgttgt gcttattatc cccacttata ttttttcgaa ggaggcacga agggttcaga
     4321 taaatatttt tccgaacttc gtgttctttg tgccttcgtg gtgagttaaa aaaatacgaa
     4381 acatgtcaaa gcattcgaag cgtcaatcga aaggaagagt ttgatcatgc tccaaggtta
     4441 tcgcgtcttc gatgccgacg ctcatggctc gatcaatccc agcatgtggg aggatttacc
     4501 ggagcagttt cgcgcccgcc ggccgcgtcc ggtgacgatc cacgacgaaa acggcctggg
     4561 caatttcacc gccggctggc tgatcgaagg ttcgctcgaa ccccacgctc tcggccccgg
     4621 cgcccagcaa gccaatacac cgcgcaatgt gttaaacgaa tttggcgcca atatgaagcg
     4681 cgagcattgt tcggtcggca gcatgacgct caacgacccg agcgcacggc tggccgatat
     4741 ggatcgtatg ggcttcgacg tccagatgat ctttccgagc acactttatg ctcacatgac
     4801 cagcgatccg gagttcgaag cggcgctgtg ccgcgcctat aaccgcttcg tcgccaaaca
     4861 atgcaccgcg gatccgcggc ggctcaaatg gtcgggcctg ctgccgttgc gcgagccgcg
     4921 ccaggcattg ctcgccatcg aggaaatgca acgcctcggc gcccgcgccg cggtggtttt
     4981 cggcaccgcc ggcgaacggc tgctttccga tccgagcttc gtgccgatct gggatgagtt
     5041 cgcccgcacc gagctgccgc tctgcataca catgggtatg agctatccgc cgctggcgca
     5101 gttgggccgg agcatttacg acggccatat tatcggcatg tcgctgccgg cccagctggc
     5161 ctttatggcc atcgttggcc accgtatgct cgaccgctat caagacttga aagtggcttt
     5221 tttagaattt ggcgccgagt ggatttttta catggtctcg cgcatggatc attatctgcc
     5281 gcttgatcgc ggcgcccatc cgttcggttt gagcatgccc aaccccggcg acctgccgcg
     5341 cgccagcatt cgcgattacg tcaaatccgg ccgcattttc atagccgccg aggccgacga
     5401 ccgcatgctg caacagttgt tcgatttgat cggcgaagat catgtattgt tctcctccga
     5461 cttcccccat ggtgaaggtc gcgataacgc agccctggaa attctcgaac gacaggatct
     5521 tgcagaacct caaaagcgca aactgcttta tgccaacacc gtgcgcttct tcggtgcgcc
     5581 gtaaagtttc gcgagggtcg actattgctg ccaatttgtc ggggacctct caccacgaag
     5641 gcgcgaagag cacgaaggtt gttcggaggc ggcttgaaac c
//
LOCUS       DASQKS010000700         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_827403, whole genome shotgun sequence.
ACCESSION   DASQKS010000700 DASQKS010000000
VERSION     DASQKS010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827403"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VE170_18135"
     CDS             <1..879
                     /locus_tag="VE170_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYJ17420.1"
                     /translation="ADAYIERGHEVHILDDFSTGQRSNVNAKATLHQIDIADSQSSKL
                     IEQIKPDVLSHHAAQMDVRHSVADPTFDARVNILGFINLLEGCKNGGVKKVIFASSGG
                     AVYGEQESFPAAESHATQPASPYGVSKRTGELYLSYYRQAFGLPYIALRYANVYGPRQ
                     SAKGEAGVVAIFLSLLLSGKVPVINGDGKQSRDYVYVGDVVAANVAALDSAFVGPVNI
                     GTGIETDVVAIYNHLRDAVGSQVNAQHGPAKPGEQRRSCIDNRRAGEVLGWRPQVALA
                     DGLQRTAAYYRENMFK"
     gene            876..2669
                     /gene="lepA"
                     /locus_tag="VE170_18140"
     CDS             876..2669
                     /gene="lepA"
                     /locus_tag="VE170_18140"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HYJ17421.1"
                     /translation="MKLDHIRNFSIIAHIDHGKSTLADRLLEFTGAVSAREKTDQILD
                     SMELERERGITIKASPVCLRYRAKDGKEYKLNLIDTPGHVDFTYEVSRSLAACEGALL
                     IVDAAQGVEAQTVANVHMALDLNLEVLPVINKIDLPSAEPERVRQEIEDVIGLDASNA
                     IFASAKEGVGIEDILEAVIHFFPPPKGDANAPLTASIIDCWFDPYHGAVVMVRVMDGQ
                     VAKGSRIRMMSSGRNFEVTRTGVYSPGPVETSGLSAGEVGFIMAGIKEVSETRIGDTI
                     TLDDNPASKALPGYKPMKPMVFSGLYPTEAHQYQALRDALDKLRLNDSSFTFEPETSQ
                     ALGFGFRCGFLGLLHLDIIRERLEREFSLSLITTAATVIYQVTKVTGEVLLIDNPVRM
                     PSEGDIQKIEEPIIDASIHLPQEFLGGVLGLCQEKRGMQKEIRYLGPKRVMLAYELPL
                     NEIVADFYDRLKSVSRGYASMEYELAGYRESDLVKLDVRINGDIVDAMSMIVHRDNAY
                     YRGRDLVQKMRSLIPRQMYEVVIQAAIGSRIVSREAVKALRKDVIAKCYGGDISRKRK
                     LLEKQKEGKKRMKQVGKVEIPQEAFLAALKV"
     gene            2682..>3193
                     /gene="lepB"
                     /locus_tag="VE170_18145"
     CDS             2682..>3193
                     /gene="lepB"
                     /locus_tag="VE170_18145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYJ17422.1"
                     /translation="MTEKVKDQMVAEGRAAPKAKSTFREYAEAILMALLLALFIRTFI
                     VQAFKIPSGSMIPTLAIGDHILVNKLSYGVRMPFLEKYLVDFGTPARDDVIVFIYPED
                     RSKDFIKRVVGVAGDNVEVRNKKVFINGKAAEDSHAHFEGYDPQLGGTVNGDNYGPKV
                     VPEGHVFVMG"
BASE COUNT          710 a          876 c          937 g          670 t
ORIGIN      
        1 gccgatgctt atatcgagcg cggccatgag gtccatatcc tcgatgattt ttccaccggg
       61 cagcgcagca atgtcaatgc taaggcaacc ttgcaccaga tcgatatcgc cgacagccaa
      121 agcagtaagc tcatcgagca aatcaagccc gatgttttga gtcatcacgc cgcgcaaatg
      181 gatgtacgcc actcggtggc tgacccgact tttgacgcgc gcgtgaacat tttgggattc
      241 attaatctac tcgagggctg taaaaacggc ggtgtcaaga aagtgatctt cgcctcttca
      301 ggcggtgcgg tttacggcga gcaggagagt tttcccgcgg cggaaagcca tgcgacgcaa
      361 ccggccagtc cctacggcgt gagcaagcgc accggcgaat tgtatctttc gtattaccgc
      421 caggctttcg ggttgccgta catcgccctg cgttatgcca atgtttacgg cccgcgccag
      481 tcggctaaag gcgaggcggg ggtggtggca atctttctgt ctttgttatt gtctggaaag
      541 gtgccggtga tcaatggcga cggcaagcag agccgcgatt acgtgtacgt cggcgatgtc
      601 gtcgccgcca atgtcgcggc cctcgacagc gcctttgtcg gtccggtcaa catcggcacc
      661 ggcatcgaaa ccgacgtggt ggcgatctac aaccatttgc gcgatgccgt gggcagtcag
      721 gtgaatgcgc agcatggccc ggcgaagccc ggcgaacagc ggcgcagctg catcgataat
      781 cggcgcgccg gggaagtttt gggctggcgg ccgcaagtcg cgctggccga cggcctgcaa
      841 cgcacggcgg cttattatcg cgaaaatatg ttcaaatgaa actcgatcat attcgaaatt
      901 tttccattat cgctcatatc gaccacggta aatcgactct cgccgaccgc ttgctggagt
      961 tcaccggcgc ggtcagcgcg cgtgaaaaaa ccgatcagat tctcgatagc atggaactcg
     1021 agcgcgagcg cggcattacc atcaaggcca gtccggtatg cctgcgctac cgcgccaagg
     1081 atggcaaaga gtacaagctc aatctcatcg acacgcccgg tcatgtggac tttacctatg
     1141 aagtctcacg cagcttggcc gcttgcgaag gcgccttact gatcgtcgac gcggcccaag
     1201 gcgtcgaagc gcagaccgtc gccaacgttc atatggcgct ggatcttaat ctcgaagtcc
     1261 tgccggtgat caacaagatc gatttgccca gcgccgagcc ggaacgggtc agacaagaaa
     1321 tcgaggatgt catcggtctc gatgcctcga atgcgatctt tgccagcgcg aaagagggcg
     1381 tcggcatcga agatattctc gaagcggtga tccacttttt tccgccgccc aagggcgatg
     1441 ccaatgcgcc attgacggcg tcgattatcg attgttggtt cgatccgtat cacggtgcgg
     1501 tggtgatggt gcgggtgatg gacggccagg tcgccaaggg ctcgcgcatt cgcatgatgt
     1561 cgagcgggcg gaacttcgaa gtaacccgca ccggcgtcta ttcgcccgga ccggtggaaa
     1621 cctccggtct cagcgccggc gaagtcggtt tcattatggc cggcatcaaa gaagtgtccg
     1681 agacccgcat cggcgatacc attacactgg acgataatcc cgccagcaaa gcgctgcctg
     1741 gttacaaacc gatgaaaccg atggtcttca gcggtctcta tccgaccgag gcgcatcagt
     1801 atcaggcgct gcgcgatgcc ctcgacaagc tgcgtttgaa cgattcgtcg tttactttcg
     1861 agccggaaac gtcccaggca ttgggtttcg gattccgctg cggctttttg ggtctgttgc
     1921 atctggacat catccgcgaa cggcttgaac gagaatttag cctgagcctg attaccaccg
     1981 cggcaacggt catctaccaa gtgacaaagg tgacgggcga agtcttgctg atcgacaatc
     2041 cggtgcgcat gcccagcgaa ggcgatatcc aaaagatcga agagccgatt attgacgcgt
     2101 ctatccattt gccccaggaa tttttgggcg gagtcctggg gctgtgccag gagaagcgcg
     2161 gcatgcaaaa ggaaattcgc tatctcggac ccaagcgggt gatgctcgcc tatgagttgc
     2221 cgctcaatga gatcgtcgcc gatttttacg atcgcctgaa gtcggtgagc cgcggctatg
     2281 cgtcaatgga gtatgagctg gccggctacc gcgaatcgga cttggtcaaa ctcgacgtgc
     2341 gcatcaatgg cgacatcgtc gacgccatgt cgatgatcgt gcaccgcgac aacgcttatt
     2401 accgcggccg cgatctagtg caaaagatgc gctcactgat cccgcggcag atgtacgaag
     2461 tggtgatcca ggctgccatc ggcagccgca tcgtctcccg cgaagcggtc aaggcgctgc
     2521 gcaaggacgt catcgccaaa tgctatggcg gcgatatttc gcgcaaacgc aaactgctgg
     2581 aaaagcaaaa ggaggggaag aagcgcatga agcaggtcgg caaggtggaa attcctcagg
     2641 aggcttttct tgcagcgtta aaggtgtaaa ttagaggctt aatgactgaa aaagtaaaag
     2701 atcaaatggt cgctgagggc cgcgcggcgc ccaaagccaa atcgacgttt cgcgagtatg
     2761 ctgaagcgat cttgatggcg ttgctcctgg cgctgtttat ccgtactttc atcgtccagg
     2821 ctttcaaaat tccatcgggt tcgatgatcc cgaccctggc gatcggcgac catatcctcg
     2881 tcaacaaact gtcctacggc gtgcgcatgc cgtttttaga aaagtatctc gtcgacttcg
     2941 gcacgccggc gcgggacgat gtgattgtct ttatctatcc agaggatcgt tcaaaagatt
     3001 tcatcaaacg ggtggtcggg gtggccggcg acaacgtcga agtgcgcaac aagaaggtct
     3061 tcatcaatgg caaagctgcc gaggatagcc atgcccactt cgaaggctac gatccgcagt
     3121 tgggcggcac cgtaaacggc gacaactatg gcccgaaagt ggtccccgag ggccacgtct
     3181 tcgtcatggg cga
//
LOCUS       DASQKS010000701         5167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_3352287, whole genome shotgun sequence.
ACCESSION   DASQKS010000701 DASQKS010000000
VERSION     DASQKS010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3352287"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..988
                     /locus_tag="VE170_18150"
     CDS             203..988
                     /locus_tag="VE170_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ17423.1"
                     /translation="MLFGGTDDTGVVRGSEMVGDRYQDLLQEIAAITEYQWRSGTTRG
                     LVKGFMLSGPPGTGKTTLAKRLAYELGRRFEAEDEPPVALAFIDGGEISRSRYGESEE
                     RIRDIFLHARSGFTAQGQRSVLLFDDVESIFMARGSQHAKEWHFSQDSVFFHSIDDLD
                     TSRATIVLTSNRPDLIDDAIRDRFLSYVVDYPDLDTLLQMVQQIPAIEQLSGTQQTAL
                     KNDLIAAVKNGTVRSMRDGQRFAMRHFVAKLLKKESLAQQIAD"
     gene            1020..2036
                     /locus_tag="VE170_18155"
     CDS             1020..2036
                     /locus_tag="VE170_18155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17424.1"
                     /translation="MAKNFYEQWLETPNKLQDELEIGRLVAADKDIPWVSTPHDVKVK
                     LMVANHLGFATMGSNVLKSEIPVGWHTGKHRHGEESMHILEGEGFSIINGQRFNWHQS
                     STLQIPFWAEHQHFNTGTVPVLIIHGMCFDLESYLRVARIEQIETCGPNEPSMIAAVP
                     EETSQYYPDGTRAIIHLEQAPTNKEKLPDGESYNPQGAIAATKNQHDFSNYLVVPKNG
                     FRAVSVAITNRWIEPGFHESGRHKHLEAVVYAIEGEGYTDMQGKRIAWQAGDVLYVPP
                     AMWEHQHINENPNSITQLRIGFNIRQWITSIWPQGFTSTRIYNDEGDPIEAGRIVRKR
                     ERSY"
     gene            2396..3859
                     /locus_tag="VE170_18160"
     CDS             2396..3859
                     /locus_tag="VE170_18160"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HYJ17425.1"
                     /translation="MNKLVGVLALVTAVLGGALVIGVQRFVPRQIEVAPATPIHIDVD
                     AAAQRLSRAIRHRTVSSVDSASPAEFERFHAFLTESFPALHKRLARETVNQLSLLYTW
                     QGTRTELKPILLLAHMDVVPADAEESPRWRHPPFAGAVAEGYLWGRGAMDDKSSLMAI
                     LEAIEWLVKQAYQPERTIYLAFGHDEETGGHSGAAKIAAQLQARRLQFEYILDEGGNI
                     LSGLLKNVSAPVALIGIAEKGYLSLALTVETAGGHSAIPPIHSAIGKLSLALAALASN
                     PFPSRLSEPTRQMLEYLGPHMAWPERFLLANLWLFESVIRHQLARSPLTNAAIRTTLA
                     PTVITGGIQENVLPSKARAVVNLRLLPGDITAAVIERVRKIIADNAISLTPLATRVEP
                     SPVTPTSSASFQILQRTVREIIPEAIVAPGLLVATTDSRHYATLSKNIFRFFPMTLRP
                     GDTERYHGIDERIGVEDYVRSVRFYVQLIRNSQSSGK"
     gene            complement(3922..4911)
                     /locus_tag="VE170_18165"
     CDS             complement(3922..4911)
                     /locus_tag="VE170_18165"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17426.1"
                     /translation="MKRINLQSFLLFYVALAAIPALGQKLDRAVLAFGSTGPNLTTFW
                     LAREAGLYKQYGLDVDVVFFRGSTIAINALASKDAHFGAFGASSSVLAKLGGMDTVLI
                     ATATPGLLFYLVTRKEIRSASDMKGKRIGASRPGTDSDLAARVAVEKLGLGEKEVNII
                     AMGTDSERLAAMAQGVIDATVVTIGGYVAAQKMGFHAILDLSQAQVPYEAASMITTRA
                     LIKENPDMVRSFTKGFVAAIQYAQSHREFTLKVLSKYMRTNDKDVLNASYDYYVGRII
                     PRVPYVSEKGLQGVIDFIRQRNPQTPNVKASEFMDNRFIKELDDSGFIKALYR"
     gene            complement(4908..>5167)
                     /locus_tag="VE170_18170"
     CDS             complement(4908..>5167)
                     /locus_tag="VE170_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17427.1"
                     /translation="NTAIQLRVDEPFRGRVSGFYGLTGGGLRAFGGMQAGLVAEWASA
                     SFAIGAGAIALAVIGYVFLGARLKKLGGLDLTITNARDSKT"
BASE COUNT         1149 a         1508 c         1357 g         1153 t
ORIGIN      
        1 cgcagcaagg cgttttgagg ccatcgtcac aagcgcggcg aggcatgcgg ttgtttccgg
       61 ctggccgcca ctcttgatat ttttgtcggt tggattagca tcatggtttc tgcttctagc
      121 tgggggatcc tactaaatgc gagcaagcga tgggcatcat catctatctg cgggaaatgt
      181 tatatcgttc gaaaagctgt cattgctttt tggcggtacc gacgacaccg gagtggtccg
      241 cggcagcgaa atggtcgggg accgttacca agacctcctg caggaaattg ccgccatcac
      301 cgaatatcaa tggcgctccg gaacgacccg aggcctggta aaagggttca tgctcagcgg
      361 tcctcccggc acgggcaaga caaccctggc caaacggcta gcctatgaac tcggtcgcag
      421 gtttgaggcc gaggatgagc cgccagtcgc tttagccttt atcgatggcg gtgagatttc
      481 acgctcccgg tatggtgaga gcgaagaaag aattcgcgat atttttctcc atgcaagatc
      541 gggattcact gcgcaggggc agcgctcagt gctgctgttc gatgacgtcg aatccatttt
      601 catggcgcgc ggcagtcaac acgccaagga gtggcatttt tctcaggaca gcgttttctt
      661 tcattccatt gacgatctcg atacgtcccg cgccacgatc gttttgacca gcaaccgccc
      721 cgatctgatc gatgacgcca ttcgtgatcg cttcctgagt tatgtcgttg actatccgga
      781 tctcgacacg ctccttcaga tggtccagca aataccggcc atagaacaac tgtccggcac
      841 gcagcagacc gcattaaaaa acgatctgat tgccgcggtg aagaatggca ccgtccgcag
      901 catgcgcgat ggccaacgtt tcgcgatgcg tcacttcgta gcaaagttgc tgaaaaagga
      961 atcgctggcg caacagatcg ctgactaatt atttaacaaa ctgacaaagg gaatcgttta
     1021 tggcaaagaa tttctatgaa caatggctcg aaacgccaaa caaacttcaa gatgaacttg
     1081 aaatcggccg cctggtggcc gcggacaaag acattccatg ggtttcaacg ccccatgatg
     1141 tcaaagtaaa actgatggtt gcgaaccatc tcggttttgc caccatgggc agcaacgtgc
     1201 tcaagtcgga aattccggtt ggctggcata ccggcaagca tcggcacggt gaagagtcga
     1261 tgcacatcct cgagggtgaa gggtttagta ttatcaatgg ccagcgtttc aactggcacc
     1321 aaagcagcac gctgcagatt ccattttggg ctgagcatca acattttaat accggcacgg
     1381 ttccggtctt aatcattcac ggtatgtgtt tcgatctcga gtcctacctc cgggttgcgc
     1441 gcattgagca gatcgagacc tgcggcccca atgagccctc gatgatcgct gccgtgcccg
     1501 aggagacgtc acaatattac cccgatggca cccgtgcgat tatccatctt gagcaggcgc
     1561 cgaccaacaa agaaaagttg cctgacggag agagttataa tccgcagggc gccatcgccg
     1621 cgaccaaaaa ccagcacgac ttttccaact atctcgtcgt gcccaaaaac ggcttccgtg
     1681 ccgtgagcgt cgcaattacc aaccgctgga ttgagccggg cttccatgag agcggcaggc
     1741 acaagcatct tgaggctgtg gtttatgcca ttgagggtga gggatacacc gatatgcagg
     1801 gcaagcggat tgcttggcaa gctggcgacg ttctctacgt gccgccggct atgtgggaac
     1861 accagcacat taacgaaaat ccaaattcga ttacccagct tcgtatcggc ttcaatatcc
     1921 gccaatggat cacgtcaatc tggccccaag gttttaccag cacgcgcatc tataacgatg
     1981 aaggcgaccc gatagaagca ggccgtatcg tgcgcaaacg ggagcgctca tactaatcaa
     2041 aattgttccg ctgctctcgt cgcccttggt ttaagacctt cttcatgttc aggctttgta
     2101 aacttggtaa cgaggcgttc ctggcaaagt cgcgcgcggc tgcaccatct acgcgcggga
     2161 cttctttccg ttgctagcct atattcaatg tcgtcagcgc tgacaatatc gtgctcagat
     2221 ctcgcctcag atatttcgcg gccaccgtca agctctgatc ccacctacct tatgacccac
     2281 ttaggccggc tcggctctta gtcgattctc gactcgtcct cgggctctgc caagtaattc
     2341 tctgcctgat caagcgactt gccgtcatct agcaaagttg ctaccccgga gctgcatgaa
     2401 taagcttgtg ggcgtgctcg ccctggtcac cgccgtcctt ggcggcgcgc tcgtgatcgg
     2461 cgttcagcgc tttgtgccgc gtcagatcga agtcgccccc gccacgccga ttcacatcga
     2521 tgtcgatgcc gctgcacaac gacttagccg cgcgattcgc caccggacgg tctcgtcggt
     2581 cgactcggca tcaccggctg agttcgagcg ctttcatgct tttcttaccg agtcatttcc
     2641 cgcgttgcat aagcgcttgg cgagagaaac cgtcaatcag ctcagcttgc tttacacttg
     2701 gcaaggcact cgcaccgagt tgaaaccgat ccttttgctt gcccacatgg acgtcgtgcc
     2761 cgccgacgcc gaagaatccc ctaggtggcg ccacccgcct ttcgctggtg cagtcgctga
     2821 gggttacctg tgggggcgcg gcgccatgga tgacaaaagc agcctgatgg caattcttga
     2881 agcgatagaa tggctcgtca aacaagctta ccaacccgag cgcactatct acttagcctt
     2941 tggccacgac gaggaaaccg gcggccacag cggtgcggca aagatcgcgg cgcagctgca
     3001 agcgcgccga ctgcaattcg agtacatact cgacgagggc ggcaatatcc tctccggcct
     3061 tttgaaaaat gtcagcgcgc cggtggcact gatcggcatc gcggaaaaag gctatttgag
     3121 tttagccttg acggtggaaa ccgccggtgg ccattctgcc attccaccaa tccacagcgc
     3181 catcggcaaa ctgagtttgg ccttggccgc cctggcgagc aaccctttcc cttcgcgcct
     3241 gtccgagcca acccgacaga tgttggaata tcttggaccg cacatggcgt ggcccgaacg
     3301 ttttctcctt gccaacctct ggctgttcga gtccgtcatt cgccaccaat tggcgcgctc
     3361 gcctctgacc aacgcggcca tccgcacgac cctcgcgccg acagtcatta caggtggcat
     3421 ccaggagaac gttttgccga gcaaggcgcg cgcggtggtg aatttacggt tattgccagg
     3481 cgacatcacc gccgcggtga ttgaacgagt ccgcaagatt attgctgaca acgccatcag
     3541 cctaacgccg ttggccacgc gcgtcgagcc ctcccccgtg acgcccacca gctcggcttc
     3601 gtttcagatc ctgcaacgca cggtgcgtga aatcattcct gaagcgatcg tagcgccggg
     3661 cttgctcgtg gcgaccaccg attcgcgcca ctatgcgacg ctatctaaaa acatttttcg
     3721 ttttttcccg atgacacttc gaccgggaga taccgagcgc taccacggta tcgacgaacg
     3781 gatcggtgtg gaggattacg tgcgctccgt acggttttat gtgcaactga tccggaacag
     3841 ccaaagttct gggaaataac cgtgaggggt tccaacccgc tctccttttt cctacactca
     3901 atctcaaggg tacagccaac ctcaacggta cagcgcttta ataaacccac tatcgtccag
     3961 ttcttttata aaccggttat ccatgaattc ggacgctttg acgttgggcg tctgcggatt
     4021 acgctggcgg atgaaatcaa ttacgccctg caggcctttt tcgctcacgt agggaacccg
     4081 cggaatgatc cggccgacgt aataatcgta actcgcgttg agcacatctt tatcattcgt
     4141 gcgcatgtat ttgctcaaga ctttcaaggt gaattcccgg tggctttggg cgtattggat
     4201 tgccgccacg aaacccttgg tgaagctgcg caccatatcg gggttttcct tgatcaaagc
     4261 gcgcgtcgtg atcatcgacg ccgcctcgta gggaacctgc gcttgggaaa gatccaatat
     4321 ggcgtggaag cccatttttt gcgccgccac atagccaccg atggtaacca ccgtggcatc
     4381 gattacgccc tgagccatgg ccgccagtct ttcgctgtcg gtacccatgg caatgatgtt
     4441 tacctctttt tcaccgagcc ccaatttctc caccgcgacc cgggccgcga gatcggaatc
     4501 ggtgccgggc cggctcgcgc cgatccgttt gcccttcata tcgctggcgc tgcgtatttc
     4561 ttttcttgtc accagataga ataacaaccc gggcgtcgcg gtagcgatca acacggtgtc
     4621 catgccgccc aatttggcga gcacggaaga gctggcaccg aaggcgccga aatgagcgtc
     4681 tttagacgcg agggcgttga ttgcaatcgt gctgccgcga aaaaacacca catcaacatc
     4741 caatccatat tgtttataca acccggcttc gcgcgcgagc cagaaagtcg tcaggtttgg
     4801 cccggtggaa ccgaaggcga gcacggcgcg atcgagcttt tgcccgagtg ccggaatggc
     4861 cgcgagggct acataaaaaa gcaaaaatga ctgaaggttt atccgcttca tgtttttgaa
     4921 tcccttgcat tcgtaatcgt taaatccaat cctcccagct tcttcaaacg cgcgccgaga
     4981 aaaacataac caatgaccgc caaagcgata gcgccggcgc cgatggcgaa cgacgcgctc
     5041 gcccattcgg cgacaaggcc cgcttgcata ccgccaaacg cacgtaaacc gccgccggtc
     5101 aaaccgtaga aaccggacac tcggccgcga aacggttcat ctacacgcaa ctgaatcgcg
     5161 gtattga
//
LOCUS       DASQKS010000702         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5025111, whole genome shotgun sequence.
ACCESSION   DASQKS010000702 DASQKS010000000
VERSION     DASQKS010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5025111"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1122)
                     /locus_tag="VE170_18175"
     CDS             complement(91..1122)
                     /locus_tag="VE170_18175"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ17428.1"
                     /translation="MARLKRRRSRLGHFFYDLRYRVGEFWLRGFIASVPYIPLRVVER
                     VTTVLAWLSFKLMSKYRRRMEENIASALGKEIPSEAVRRDLVWRAWKNFARGVFDTTA
                     IIHRSKARIVDSIRLDGEEHLQNALANGKGVLALSAHLGGFTMIGARLAAAGYPFSVV
                     VKHPADERFARLTDDYRSQVGIHTIPAKPRRQAARGIIKALRENRIVLVIADEFKSGD
                     VMVDFFGMQLPAPRGPATLALRTGAVTLPMFATRELDDSLLLAVGPAIPTVEREDLEA
                     SVVATTATYTGYLEAAIRRYPDQWNWLGLPNRNGKLSRAELALRRKERMKDAAAREVT
                     LTRKTGTGV"
     gene            complement(1232..1690)
                     /locus_tag="VE170_18180"
     CDS             complement(1232..1690)
                     /locus_tag="VE170_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17429.1"
                     /translation="MAYENGLSMDERVTSLFQPDTLLPDQYLDTFRRKLHLEPEKKLM
                     LAILEDAVACFQKYLLARDSKGKAQFHDTEAWVFQRDDSGVFSFDIVCETLGLDPDYL
                     RRGLAQWQQRRVTQQTQAQVIQLRPARGKPRRSMPISGRPKPRMRRAASH"
     gene            complement(1754..2209)
                     /locus_tag="VE170_18185"
     CDS             complement(1754..2209)
                     /locus_tag="VE170_18185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYJ17430.1"
                     /translation="MGLMQIPRRVDYGLRAVIYLSAQDRDKCCSIAEISQQQGIPRKF
                     LEKIIQDLVRGGLLKSKRGSCGGYALARAPEEISFNDVIEAIDGPIAVNVCLDDHLGC
                     DQMPRCTMIGVWSEVQQKINAVLCGTSIAGLRRSPCRDFIGSSSLSSAT"
     gene            complement(2227..>2846)
                     /locus_tag="VE170_18190"
     CDS             complement(2227..>2846)
                     /locus_tag="VE170_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYJ17431.1"
                     /translation="ISGHKFHAPKGVGALYVRRGITFPPFIIGGHQERNRRAGTENVA
                     GIVGIGQAAEIALNRIAADSEHERQLRDRLEALLLATCPESRVNGRNQERLPNTLNIS
                     FRYLEGESMLVLLDQQGICASTGSACTAGSAEPSHVLRAMDLPADWLQGAVRFSLSRF
                     NSEAEVNYVNEKVPAIVQRLQGLSALGRLGSQGRPRDERSVAGTR"
BASE COUNT          609 a          863 c          800 g          574 t
ORIGIN      
        1 agaacaaata ccgcttcatg cgctacttgg aggcgagcga gaacaaaggc attctgaatc
       61 cgggattcga ctcgaaaata taattggcgg ctagacgccc gtgccggttt ttcgcgttaa
      121 agtgacttcc cgcgcggctg cgtctttcat ccgttctttc ctacgcagcg ccagttcggc
      181 gcgcgacaac ttaccattac gattgggtag gccgagccag ttccactgat cgggatagcg
      241 gcgaatcgca gcttcgagat aacctgtgta ggtcgccgtg gtcgccacca cactcgcttc
      301 gagatcttca cgctccaccg tggggatggc aggacccacc gccaatagca aagaatcgtc
      361 gagctcgcgg gtcgcgaaca tcggcaacgt gacggcgccg gtgcgcaggg cgagagtcgc
      421 cggaccgcgc ggcgccggca gttgcatgcc aaaaaaatcc accatcacgt cgcccgactt
      481 gaattcatcg gcgatgacca gcacgatgcg attttcgcgc agcgccttaa ttataccgcg
      541 cgccgcttgg cgccgcggtt tggcgggaat cgtgtgaatg ccgacttgcg accggtagtc
      601 gtcggtgagc cgggcaaacc gttcatcggc cggatgtttc accaccacac tgaagggata
      661 gcccgccgcc gccagacgcg cgccgatcat ggtaaaaccg ccgagatgcg cgctcaacgc
      721 gagaacgcct ttgccgttgg ccaaggcgtt ttgcagatgt tcttcgccat cgagccggat
      781 gctgtcgacg atccgcgctt tcgaacgatg tatgatcgcg gtggtatcga atacgccgcg
      841 ggcaaagttc ttccaagcgc gccataccaa atctcgacgc accgcctcgc taggaatttc
      901 cttgcctaag gccgaggcaa tgttttcctc catgcgacgg cggtacttcg acatcaactt
      961 gaaagatagc caagccagca ccgtggtaac ccgctcgacg actcgcaagg gaatgtacgg
     1021 cacagaggcg atgaatccgc gtaaccaaaa ttcgccgacg cggtagcgca ggtcgtaaaa
     1081 aaaatgtccg agccgcgagc gccgccgctt taacctagcc atacatgatt tatgaattcg
     1141 tttggcgggc ttttcaagtt ctcgcgaaag cgaaaccggc agcgctgggt catcccagcg
     1201 ctgccggtgc taaggtgagg ccaataaccg gttagtggct ggccgcccgg cgcattcgag
     1261 gtttaggccg gccggaaatc ggcatgctac ggcgcggttt accccgcgcc ggccgcaact
     1321 gaatcacttg cgcctgggtc tgctgggtga cgcgccgctg ttgccattgt gccagaccac
     1381 ggcgcagata gtcaggatcg agtcccaatg tttcgcagac gatatcgaag gagaagactc
     1441 ctgaatcgtc gcgctggaaa acccaagctt cggtgtcgtg gaattgcgcc ttgcccttgc
     1501 tgtcacgggc aagtaaatat ttttgaaaac aggcgacggc atcctctaaa atcgccagca
     1561 tgagcttttt ctcgggctcc aagtgcaatt tgcgccggaa tgtatcgaga tattgatccg
     1621 gcagcagcgt gtccggctga aacagtgaag tcacccgctc gtccatactc agtccattct
     1681 cataagccat gaaaaccacc tccccttcgg atgcggccca gaacccggct gcagcacgaa
     1741 ctttcgcatc cggctacgtc gcactcgaaa gagaagagga gccaataaag tcccggcagg
     1801 gcgaacgtcg caagcccgca atcgacgtcc cacagagcac tgcgttaatc ttctgctgca
     1861 cttcgctcca cacgccgatc attgtacagc ggggcatttg gtcgcagccc aggtgatcat
     1921 cgaggcatac attgaccgcg atcggtccgt cgatcgcctc gatgacatcg ttaaaagaaa
     1981 tctcctcggg cgcacgcgcc agcgcataac cgccgcagga accgcgtttc gatttgagca
     2041 gcccgccgcg aaccaggtct tgaataatct tttccaaaaa cttgcgcggt atgccctgct
     2101 gctgagagat ttccgcgatg gagcaacatt tgtcgcggtc ttgagccgac aaataaatta
     2161 ccgcgcgtag accgtaatcg acccgccgcg gtatttgcat gagccccata tatttttccc
     2221 tcgttgttaa cgcgtaccgg caacgctgcg ttcgtcccgc ggccggcctt ggctgcccaa
     2281 ccgcccgagc gccgatagcc cttgcaagcg ttggacgatc gccggcacct tttcgttgac
     2341 ataattgact tctgcttcgc tattgaaccg gctcaagctg aagcgcaccg ccccctgcaa
     2401 ccaatcggcc ggcaggtcca tcgctcgcag gacatgtgac ggctcagcgg agccggcggt
     2461 gcaagccgag ccagtggaag cgcagattcc ttgctggtcg aggagaacca gcatcgactc
     2521 gccttccaga taacggaagc tgatgttcaa ggtattcggc aagcgctctt ggttgcggcc
     2581 attgacccgg ctctcgggac aagtcgccag caacagagct tccaaacgat cgcgcaattg
     2641 tctctcgtgt tcactgtccg ctgcgatgcg attcagggca atctcggcgg cttggccgat
     2701 gccgacaatg cccgcaacat tttccgtgcc ggcgcgccga ttgcgctcct ggtggccgcc
     2761 gattataaac ggcgggaaag tgatgccgcg gcgcacatac agcgcgccga cgcctttcgg
     2821 tgcatgaaat ttatggccgg agatcg
//
LOCUS       DASQKS010000703         6421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_827993, whole genome shotgun sequence.
ACCESSION   DASQKS010000703 DASQKS010000000
VERSION     DASQKS010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827993"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1080..1346
                     /locus_tag="VE170_18195"
     CDS             1080..1346
                     /locus_tag="VE170_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17432.1"
                     /translation="MKYITTTALIFTFFFFLTTYARSEEHYIYKDGQGKLVISNQRPP
                     VGSNILRIMDLPEFREVQMQPVQESSSARSTVRLESSAKPEQKK"
     gene            1950..2246
                     /locus_tag="VE170_18200"
     CDS             1950..2246
                     /locus_tag="VE170_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17433.1"
                     /translation="MGIALFGLIVVSVAIGIQAQITKGRTGAGWGFLTFVVLFIIFTT
                     LWPNRTMVTTQAQPRATSTTSRDIFGPTGFAAAVAIVLGGPLMAVIVSTLPKVK"
     gene            complement(2416..2784)
                     /locus_tag="VE170_18205"
     CDS             complement(2416..2784)
                     /locus_tag="VE170_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17434.1"
                     /translation="MASSKVNRLGTKPELNAQVAKKILGWKNVHRDHKRGNSDEVLAK
                     KADKAGRWRQTKVPNFSGDIGNIPIIETRLQQLGLFDLYTKELAKIALAKELPVDWAT
                     PDQRCKAALTVVGKRSKRLK"
     gene            complement(3239..3508)
                     /locus_tag="VE170_18210"
     CDS             complement(3239..3508)
                     /locus_tag="VE170_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYJ17435.1"
                     /translation="MGIIGWIFFGLIVGIVGKFLMPGRDPGGWVVTILLGIGGALIGG
                     FVGQALGFYREGEPAGFIMAVLGSILLLVIYRMFAGKRTISDRTY"
     gene            complement(3697..4689)
                     /locus_tag="VE170_18215"
     CDS             complement(3697..4689)
                     /locus_tag="VE170_18215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ17436.1"
                     /translation="MALRKWARHSRRIDFTGKTVVISGGSRGLGLELARQFADRGANL
                     VLLARDEGNLLRSASELETLGVQVRPIICDITRSADVQQAVQSIMRQTGRIDVLINNA
                     GIIQVGPMENMTAEDYQQSMRVHFCGPLSLIQQVVPVMRAARFGRIVNIASIGGKVAV
                     PHLLPYVASKFALVGLSEGLRAELLKDGIYVTTVAPGLMRTGSHLQALFKGQYKKEYA
                     WFALAGASPLLSTSAPSAARKIVEACRYGDAEITITAQAQLLRIAHGIFPGLLADLLG
                     LADRLLPRPNGDNLSRKGWESGSALAPSFLMRSANRAAAINNELPEQVSTGINK"
     gene            complement(4938..5546)
                     /locus_tag="VE170_18220"
     CDS             complement(4938..5546)
                     /locus_tag="VE170_18220"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HYJ17437.1"
                     /translation="MPNATQMLRQDHRKVEAMFNKFEQGKAMNAKRAVAQQAMNELEV
                     HAQVEEEIFYPAVKRAIESSELVDEAKKEHQEAKSLISQLKRAGGQDDGEGSEFESMF
                     AELMQAIKHHVEEEEGEMFPQVEDSELDLAALGAQMMKRKQQLTKGLGSPSAKSRTRS
                     KSPSKSKSTLKTKSRSKTKSRSKTKSRSKTTRGSGRKRARAR"
     gene            complement(5587..>6421)
                     /locus_tag="VE170_18225"
     CDS             complement(5587..>6421)
                     /locus_tag="VE170_18225"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYJ17438.1"
                     /translation="PMLVIAISVAGFFFGREAAQNQIVETLSGFVGTDAGVVIQTMIE
                     NASKQQTTDSIFAMLVGVALLLFGAGGVVGQLQSSLNMIWGVEPRPDRGILGFIKERF
                     ISYGMVLGVGFLLLVSLVVSAALSAFDKFLGGAAPGASLVLQVIHFLVSLSLIAFMFA
                     AIYRFMPDGRIAWRDVAVGGILTALLFNVGKFFIGLYLGQSSVTSVYGAAGSLVTLLL
                     WIYYSSLIFFFGAELTQVYATRFGSGLIPSPNAQPAAPEIKRGQRVRRDDNRSAVRPT
                     R"
BASE COUNT         1629 a         1673 c         1604 g         1515 t
ORIGIN      
        1 tcggcgcgcg gcccatccgt cggtacgctg caccgaacaa attggatgat cattatcatc
       61 gcaaaaaatt gactcaatag atgtgccaac actgcggctt caaacctata aagcatgcac
      121 gaaaggatgc gccgagaaga gattgtcgtc gtctatagag tcccaacact gagactaaaa
      181 ttaccaagag gatgccaagg ctgagacgca ctctattttg ccgttacgcg ccagttccgg
      241 agcactgctt agttttatta agctcaagca aattgattgc ttataggtcg tattgcgatt
      301 cgcttattac ctgactttat tatccccctg gactgacaat tgcatttcag cttgccgctg
      361 gtcatgcgca gcgccgttat tgtctttagt cgtcaagggg tttacagacg agacgataat
      421 gttcgaagtg tacaagggct atttgatcaa gatcatttcg tctcgattat ccaaccgcca
      481 taaatggcga gcaaacgtaa tagtgcattg gtacgagggt aacgccaaga gaacgcaacc
      541 cctagacggt cctgcctctg gctttgaaag tagaaaaaaa gccgactcgt ggggaataca
      601 atctggaaga aagtggatcg ataatgggat gcccaatcca gacagccaag gctaaaagtt
      661 tttccatact cgtaatgacc ccggtctctg ctttctcctg tgttgggaat gagacttgga
      721 acggcttatg gctccggtac tctaattatt gagtcgagcg cacgattaaa atcaatcatt
      781 aacgaaggta tccgaatcta tcgaacggaa ggagggatcg cgaaatggcc gaacagaact
      841 gtgctcacag tgggtgtaac tgcaaagtgg aacaggacaa aggcgtaacc aaaggggcaa
      901 acacatattg tagcgaactt tgcgccaatg ctccgtcggt ggctgcaggc aaatgccaat
      961 gtaaacaccc cggttgctag taaaataaac agagggaggc gctttaatgt cgcctacttc
     1021 ctcacgagaa ttgccagcgc ctgtggggta tggagttggc gcaacaacgg agatcacctg
     1081 tgaaatacat cacgactacc gcactgatat ttacgttctt ttttttcctc actacctacg
     1141 cccgatccga agagcattac atttacaaag atggccaagg caagctggtc atctcaaacc
     1201 aacggccacc cgtcggaagc aacatcctac gcataatgga tctaccggaa ttccgtgaag
     1261 ttcaaatgca accggttcaa gagagcagca gcgcgcgttc caccgtcagg ttggaaagct
     1321 cagccaaacc ggagcaaaag aaataaacac ctggctcgtc gaatagagac actagactta
     1381 aacgcttgcc aatgatcaca aaaagcacgt ctttatccgc agcgaaaaaa ttgtggggta
     1441 tttatgtttc tgccttacaa gggtcattcc atcggtacca ttccgtggcg gctaccgaac
     1501 agtaataaat ggcgagccaa agtgatcgtg caatgggacg acggccatag tgtcagaacg
     1561 caacactttg atagtcctgg caatggcttc ggaagtgaag aaaaagccga tttgtgggct
     1621 atagcgactg ggagaaagtg gatcgatgac ggtaagcccg ttatcgagac agcgcatata
     1681 caagatccac taccgagtgg ataagatctc gtctacgagc ggacgagcaa ccgcttcgag
     1741 acactccggc ccgccgctat aagcgagtga gcgctcggcg accacaggag atccatgcgc
     1801 aacgaggcca tgaaagatta aaagccaact caggcacgac tatcaggtac gaatatctag
     1861 accatgtctt cgagtaatgg aactcaaatc gccacggcag gatcgagtca gcgagatttc
     1921 gccgttcgtg aacacaaccc gaggctgcta tgggcatcgc gcttttcggc ttgatcgtag
     1981 tcagtgtggc gatcggcatt caggctcaaa tcacaaaagg tagaaccggc gccggctggg
     2041 gatttttaac ttttgtcgtt ttgtttatta tcttcactac gttgtggcca aatcgtacga
     2101 tggtgactac gcaagcgcag ccacgcgcga catcgacgac gtcacgggat attttcggtc
     2161 cgactggctt cgccgcggcg gtcgcgattg tgctaggcgg gccgctcatg gcggtcatcg
     2221 tatctacgct gccgaaggtt aagtaacaac tcgtccggct gcatgaggct atgacgcctc
     2281 atgacgcggg cgtccggtgc tttttgcaca cccggcagtg gctccaatga ctcattgata
     2341 agcccttggg gactgccgca gctgtataac cagccactag tagttaccgg gcccgcaaca
     2401 gcgactacaa attatctatt tcaatcgctt cgagcgcttg ccgacgacag tcagagccgc
     2461 tttacagcgc tgatcaggcg tggcccagtc aaccggtagt tcctttgcta aagcaatctt
     2521 ggctagttcc ttagtataaa gatcgaagag tccaagctgt tgcagacgag tttctatgat
     2581 aggtatattt ccgatgtctc ctgaaaagtt aggcactttg gtttgacgcc atcgcccggc
     2641 cttatccgcc ttcttggcta gaacttcgtc cgagttgccg cgcttgtggt cgcggtgaac
     2701 attcttccag cctaagattt tcttagcaac ctgcgcgttt agttcaggct ttgttccgag
     2761 tctgttcacc ttgctgcttg ccatgggtgc tcagaacctc agtagaaagg aaggtcgttc
     2821 acgtcaaagt tgattgttaa ggatctcgga aagctttaat tacatggtcg attttagaaa
     2881 cggcgggtgg gcaaaaatgc ttggcaacgt cgaggagcat ttccgcctcc gcaacgtcac
     2941 aaggtatagc gatagtatcg gccccgatcc tgatcgaatt tttcaatctc aagtacaccg
     3001 gggaggattc attggcgtat cttaagatcc aattaaattc ctccgaagat atttcaatca
     3061 ataatttgtc agccgaccga gtagattttt gtgctctcat agatgaagtc tttacctggg
     3121 aaatgtcgga gtgaaggtgg aggggagtat ttataaaatt aaaaatgcac tcaaacttgc
     3181 agaagcccgc caataaatta acgggcttct gcaacgatca gtttgaatat taagctcttc
     3241 agtaagtccg atcagagatc gttcgtttgc cggcaaacat tcgatatata accaagagca
     3301 gaatagagcc gagtactgcc atgataaaac ctgccggctc accctcacga taaaagccaa
     3361 gcgcttggcc gacaaatccg ccaattagcg caccacctat tcccaacaat atagtaacga
     3421 cccatccacc gggatcacga cccggcatta aaaacttccc cacaatcccg actatcaacc
     3481 cgaaaaatat ccatcctata atacccataa gacctcctgt ttatactggt cgaactggct
     3541 tgaggtagtg caaagttagt accattcggt agacaacttt gttgttaaat ttcacctgaa
     3601 taaaccattc gcatctcatt agtgaagcga aaggagaatt cattctcgat aatgagacag
     3661 gttctttctt tgcctcacca tcaggtcaac agaaacctat ttattgatac cggttgaaac
     3721 ttgctcggga agctcgttgt tgatagcggc cgcgcgatta gcagatcgca tcagaaagga
     3781 tggggccagg gcggatccgc tttcccagcc cttgcgcgaa agattgtcgc cattaggcct
     3841 aggcagaagc cggtcggcga ggccgagtaa gtccgcaagt aacccaggaa aaattccgtg
     3901 cgcaattcgc agcagctgcg cttgcgcggt gattgtgatt tcggcgtcgc cgtaacggca
     3961 tgcttcgaca atttttcgcg ctgccgatgg tgccgatgtt gaaagcaaag gagaagcacc
     4021 ggcgagagcg aaccaagcgt attccttctt atattgtcct ttgaacaaag cctgcaaatg
     4081 agagccggtg cgcattagtc caggtgctac tgtcgtgacg tatatgccgt ccttcaacaa
     4141 ctcggcccgt agcccctcgg ataaaccgac cagggcgaat ttgctggcca cgtaaggaag
     4201 caaatgcggc accgctactt tgccaccgat cgatgcgatg ttcacaattc gaccgaagcg
     4261 cgcggcccgc atgacgggca cgacctgttg tatgagagaa agcgggccgc aaaaatgcac
     4321 ccgcatgctc tgctggtaat cttcagcggt catgttctcc ataggaccga cttggatgat
     4381 gccggcatta ttgatcagga catcgattcg gccggtttgg cgcatgatgg attgcaccgc
     4441 ctgctgaaca tcggcactcc gagtaatgtc gcatatgatg ggcctgactt gcacacccaa
     4501 ggtttcgagt tcgctcgcgc tgcgcaacag gttgccttca tcgcgcgcca gcaaaaccag
     4561 gttcgctccc cgatcggcaa actggcgtgc taattccaag cctaacccgc gcgacccgcc
     4621 cgaaattact acggtcttac ccgtaaaatc tatgcgacga gagtgtcgtg cccattttcg
     4681 cagggcgata agcgagccgc cggtaacagc taaggcagtc aatcgacgat agttttgcgt
     4741 catcgcggtt ttccaagcac ggggttcgaa caaaccttat ctcgtaaaac gggttggtga
     4801 acggattgtc agaacctgca atatcccagc gcaataagct ccaacatcat gatgcgaccg
     4861 agttcgctct tgtaggcagc tgtttcgcca gagagtgcgc cggccgccgc actctctggt
     4921 tgttttgcgc cactcgatta gcgcgcccgc gcgcgtttcc tgcccgagcc gcgtgtggtc
     4981 tttgaccggg acttggtttt cgatcgcgac ttggtcttag agcgcgattt ggtcttaaga
     5041 gtcgatttag atttgcttgg ggacttcgag cgggttctgg actttgcgct cggcgatccc
     5101 agacccttgg ttaattgttg cttacgcttc atcatctgcg cccctagggc agcgagatcc
     5161 agctcgctat cttcaacttg aggaaacatc tcgccttctt cttcctcgac gtggtgcttg
     5221 atcgcttgca tgagttcggc aaacatcgat tcaaattcac tgccttcacc gtcgtcttgc
     5281 ccgcctgctc ttttgagctg gctaatcagc gactttgcct cctgatgttc tttttttgcc
     5341 tcatcgacca actcgctcga ttcaatcgcc cttttgacgg cgggataaaa aatttcttct
     5401 tctacttggg cgtgaacttc aagctcgttc atggcctgtt gggcgacggc tcttttggca
     5461 ttcatagcct tgccctgctc aaatttgttg aacatggctt ccaccttcct atggtcctgg
     5521 cgtagcattt gcgtcgcgtt cggcataaaa attttctcct ttgaaattag aattctgcca
     5581 ataatgttaa cgcgtaggtc tgacagcact ccggttatca tcacgccgca cccgctggcc
     5641 ccgtttaatt tcgggcgcgg ccggctgtgc gttgggggaa gggattaatc cggatccgaa
     5701 tctggtcgca tacacctgag tcaattcggc gccgaagaaa aaaatcagcg aagagtagta
     5761 gatccataag agcaaggtga ccagcgatcc cgcggcgcca tagaccgagg ttaccgagct
     5821 ttgcccgagg taaagcccaa taaaaaactt gccgacatta aatagcaaag ccgtgagaat
     5881 accgccgacc gctacgtccc gccaagcgat acgcccgtcg ggcatgaatc gataaatcgc
     5941 ggcaaacata aatgcgatca ggctaaggga gacgagaaaa tggatgacct gaagcaccaa
     6001 actggcgccg ggtgccgccc ctccaagaaa cttatcgaag gccgaaagcg ccgcgctcac
     6061 gaccaaagaa accaataaca gaaagccgac cccgagcacc attccgtatg agatgaagcg
     6121 ctccttgata aaaccaagaa tgcctcggtc tggtcttggt tccacccccc agatcatgtt
     6181 caacgagctt tgcagttggc cgaccacgcc accggcgccg aataagagca aggccactcc
     6241 caccaacatg gcgaaaatgc tgtcggtggt ctgctgcttg ctcgcgtttt cgatcatggt
     6301 ctgaatgact acgccggcat ccgtgccgac aaaacccgaa agcgtttcga cgatttgatt
     6361 ttgtgcagct tcgcggccga aaaagaaacc cgcgacggat atcgcaatca ccagcatcgg
     6421 g
//
LOCUS       DASQKS010000704         8225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2939856, whole genome shotgun sequence.
ACCESSION   DASQKS010000704 DASQKS010000000
VERSION     DASQKS010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2939856"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1544
                     /locus_tag="VE170_18230"
     CDS             60..1544
                     /locus_tag="VE170_18230"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ17439.1"
                     /translation="MLTLDEIIAKLTDIVGPRRISTNPIDKINYQHTLAYGAYRALPD
                     VIVRIESDAQGNHKVADILKFANEHKIPIVAKGGVGMGISSPLKGGLLLDMLGMDKIL
                     EVNSTMQYVTAEGGASVYAIHHALRQHGLMLPNFGTYGPAVVIGAMINKGGIGYGMTR
                     YGWIADMVLGLEVILANGETIRLGALANQGTTFGPFQKWIHLPDLLGLFTLAAGSMGI
                     ISRVCLRVIQCQREWLHDYCYVFRRDQLQQVQDSLVALVRDEIVYDIHFTDRWQYHWP
                     MAEGLYDKNKIPDDGWFFLKTVVMARDAEELAFKEKRMRRIYESAGGVDVPEIADKAM
                     GAKAQELGLHGWPDWHVMGPDGWCGTVVRHTGMFAVTSKMYPLRFLKEMYDLDEAAKR
                     ECGIWDAEHAPQHDSYVTRDLAIKEEYFTFYNPYDDEDVARLRKWMGMVAQFSNERGV
                     VWTAPTLSTKGPVPYNRLGSLYDVVKEIKQLVDPNNILNPGALY"
     gene            1632..2426
                     /locus_tag="VE170_18235"
     CDS             1632..2426
                     /locus_tag="VE170_18235"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ17440.1"
                     /translation="MGLGRQDFSGRKLSPQEADAVIADLKKYLLDLADRWLIKGRFHS
                     EMGTGKLPMEAIKVFWQNWHGFVAEINNFHGVAYQRHLPFFKRHPELQKHFAAHVADE
                     MIHPKPPGHIQIVLEQGRNFGLSDEEMVEYEMLAECRAFLEYYRGVLYEGTMVEWWAS
                     FCVEEAVGHWARFFGTALRKVYDFPEEKIVYFRVHAKADLEEHDGIMAHADLDWMVLK
                     NLLIEGKAETRPGFNLEYCMRMGTAYFAWFFEGVYQQINKEGLYKE"
     gene            2428..3453
                     /locus_tag="VE170_18240"
     CDS             2428..3453
                     /locus_tag="VE170_18240"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ17441.1"
                     /translation="MAESDQEEMSPRPLMRILGDFANSQILDAALEYDFFTLISKGFD
                     SAEALAREAGTDARATRIVLDSLPALSLIEKRDGKYFLTPSAQAFLVKGRPSYCGDFR
                     HVALALWDGMAHLKESLKTGKPLSRMDTGTELPVWEKLVLGIIVIAEPAAKALCDILK
                     IGSERKGLRVLDVAGGSSIFGMTIVSRDPTAQVTQLDWPNVNAVAQKANKERGLEGKI
                     RFIDGEHHTANIETNHYDLVLASNFCRFESPKGNQELFAKAFSALKAGGIFVVNDFVP
                     NEERTEPTFALRFSVYTLTHTPAGECWTLAQYSDWMKKAGFNSIATHGDISKTLPGTT
                     LILATKS"
     gene            3925..5331
                     /locus_tag="VE170_18245"
     CDS             3925..5331
                     /locus_tag="VE170_18245"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HYJ17442.1"
                     /translation="MQYLAICEDDLLFQFMRNVATEQGDVRYLVPNAKIAQRIRRQRG
                     KVWHGDLLKEETYKKTKLHHVDQAIVFVRDADYQHRVCRLLRVLDKNIPIVSLTSEKN
                     GKKPAKEPDDPLLRHLQLADIFAEFCATQLLDTLNIKKVERIRSLFNDGEKIHILLQH
                     DPDPDAIGSALALRELLGRNRTTTPIVTFGTVTRPENLAMIELLEIQIEQITPDELYQ
                     DGARLALVDVQPPYFEQPLRRVDLVVDHHPKRSKFKARFADLRTGYGSTATIFTEYLR
                     AAGMEPSQRLATALLYGIKTDTLFLERGSNLADLSAFNYLYPIANKTIIARIERPALP
                     REDLEAMGRALTHLQVDSSVAVIHLGEVNREDVIPQMAEFCLQIEGVDWGVVSGLVNN
                     RVVISVRNVGYVKSAGEIMKKLYDDIGSAGGHRAMAKAVVPLDRFKERFGEVSEKVIR
                     EAMLPLIVDKDQIELELR"
     gene            5488..5841
                     /locus_tag="VE170_18250"
     CDS             5488..5841
                     /locus_tag="VE170_18250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ17443.1"
                     /translation="MECINLGQKIEAARAGKIKNNHFFGTDNFRSWMLYFEPGDDTPM
                     HFHQNPETFLVVQGECSIKDFKGGERVAKKDDIVFFPAKEYYQLINKGTEPLVMFGNR
                     SEPFGIGITRAEAAK"
     gene            5877..6269
                     /locus_tag="VE170_18255"
     CDS             5877..6269
                     /locus_tag="VE170_18255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ17444.1"
                     /translation="MPKLRHLAIKTEDTGKLATFYMDVFEMQVLHKDRKEGGAVYLTD
                     GYFNLAILPNNEQQSPNGLYHFGFEVENGEKIVERLKKFNPNKMPKQRPNGRPYAETR
                     TSDIDGNFFDISEHGFQVVQPVGGKEEN"
     gene            6305..6697
                     /locus_tag="VE170_18260"
     CDS             6305..6697
                     /locus_tag="VE170_18260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ17445.1"
                     /translation="MPKIKHLAIRTEDTGKLAGFYMDVFEMQMIHKEKDEGGAIFLTD
                     GYFNLAILPNHEQNAPNGLYHFGFEVESGEKIVDRMKKVNPNKLPKSRPNGRPYAETR
                     GSDPDGNYFDISEHGFQNVVPLGEKKSD"
     gene            6790..7827
                     /locus_tag="VE170_18265"
     CDS             6790..7827
                     /locus_tag="VE170_18265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17446.1"
                     /translation="MTRITLPHGRRRLPGACGMFVTLSLVFCLPGTSSGQPKSNLSSS
                     VTSESMTHVWVARERGLFKKYGVDMQFVLMPRNPLAIAALLAGEIDAAIIGPGHLVNA
                     GLSGAELAGIANFNQKLDFRLNTRPDIKKPEELRGKRIAVSGPGSTSHLVSMLALQGL
                     NLDPVQAKISFLTIPGTEMNRRLALESGSVDATTLRGAMGEVYANKGYPLLYNLNSAG
                     VTLPQNMMVITRRAAATKPQVIEGHLKATIEAIAMINDPANRELVSRLLATNLRLSNP
                     ADIEESYQTVITNYERAPHPSVEGMKRLQKLLIQLNPKIADVRVETLIDNSFMNKLES
                     SGFIQGLYKKN"
     gene            7876..>8225
                     /locus_tag="VE170_18270"
     CDS             7876..>8225
                     /locus_tag="VE170_18270"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ17447.1"
                     /translation="MRTENIPVVVVGGGSAAFEAAVAAKQSGAAKVVMLEKAPEPEFG
                     GNARYSHTGFRWVFSGADEIRRFLPEVDDAMFAKLNLPAYSSELFHADLQRVTRGRID
                     QTLADVLVSQSNAA"
BASE COUNT         1930 a         2233 c         2282 g         1780 t
ORIGIN      
        1 atctgcctac aatagccgag gcctaaattt ttgattgttc gccctccatt gttttttcca
       61 tgcttaccct cgatgagatc attgcaaaac tgaccgacat tgtcggcccg cggcggattt
      121 cgacaaaccc aatagacaag atcaactatc agcacactct ggcttacggc gcctatcggg
      181 cgctaccgga cgtgatcgtc cgtatagagt ccgacgccca gggcaaccat aaggtcgccg
      241 acatcttgaa attcgccaac gagcacaaaa ttcccatcgt cgccaagggc ggcgtcggta
      301 tgggcatcag ctcgccgctc aagggcggtc tgttgctcga catgctcggc atggacaaga
      361 ttctcgaagt aaattcgacg atgcaatatg tgaccgccga ggggggcgcc agcgtttacg
      421 ctattcatca cgccctgcgc cagcatgggt taatgctgcc caatttcggc acctacggac
      481 cggcggtggt cattggcgcg atgatcaaca aaggcggtat cggctatggt atgacccgtt
      541 acggctggat cgccgacatg gtgctcggct tggaagtcat tcttgccaac ggcgaaacca
      601 ttcgtttggg cgcgctggcc aatcaaggca cgacgttcgg gccgtttcaa aaatggattc
      661 acctgccgga tctgctcggt ctattcaccc tcgccgccgg ctccatgggg attatcagcc
      721 gagtttgtct acgcgtcatt caatgccagc gagaatggct gcacgattac tgctatgtgt
      781 ttcgccgcga tcaactgcag caggtgcagg attccctggt tgcattggtt cgggacgaaa
      841 tcgtctacga catccatttc accgaccgtt ggcagtatca ctggcccatg gccgaggggc
      901 tatacgacaa aaacaaaatt cccgacgacg gttggttttt cctaaaaacc gttgtcatgg
      961 cgcgtgacgc ggaagagctg gcgttcaaag aaaagcgcat gcggcgaatt tatgaaagcg
     1021 ccggcggcgt cgatgtgccc gagatcgccg acaaagcgat gggggcgaag gcgcaggagc
     1081 tgggtctgca tggctggccc gactggcatg tcatgggacc ggacggttgg tgcggtacgg
     1141 tggtgcgcca caccggtatg ttcgctgtga cttcgaaaat gtatccgttg cggttcttga
     1201 aagagatgta cgatctcgac gaagcggcca aacgcgagtg cggcatctgg gacgccgagc
     1261 acgcgccaca acatgatagt tacgtcaccc gcgatttggc gatcaaagaa gaatacttta
     1321 ctttttacaa tccctacgat gatgaagatg tcgcccggct gcgcaaatgg atgggcatgg
     1381 tggcacagtt cagcaacgaa cgcggcgttg tctggaccgc gccgactttg agcaccaagg
     1441 ggccggtgcc ttataaccga ctcggcagtc tctacgacgt cgtcaaagag atcaaacaat
     1501 tggtcgatcc caataatatt ctcaacccgg gggcgctcta ttaggtccgt tcaaaattca
     1561 agcactcaat aggttcaaga aaacagaaat cgggaaagat tcgtatcggc agagttcgta
     1621 aaggggggat tatgggatta ggcagacagg atttttctgg caggaagctt tcgccgcagg
     1681 aagccgatgc ggtgatcgcg gatttgaaga aatatttact cgacctagcc gatcgctggc
     1741 tgatcaaagg tcgtttccat agcgagatgg gcactggcaa gttgccgatg gaggcgatca
     1801 aagtgttctg gcaaaattgg catggctttg tcgccgagat caataatttc cacggcgtgg
     1861 cttaccaacg ccacctgccg tttttcaagc gccatcccga gctgcaaaag cattttgccg
     1921 cccatgtggc cgatgagatg attcatccca agccacccgg ccatattcaa atcgttctcg
     1981 aacaaggacg caatttcggg ctctccgacg aggagatggt cgagtatgag atgctcgccg
     2041 aatgccgcgc gttcttggaa tactaccgcg gcgtgcttta cgagggcacc atggtcgagt
     2101 ggtgggcgtc gttttgcgtc gaagaagccg tcggccattg ggcgcgcttc ttcggcacgg
     2161 cgctgcgcaa agtttatgat ttccccgaag aaaagatcgt ctactttcgg gttcacgcca
     2221 aggccgattt ggaagaacat gacggcatta tggcgcacgc cgatcttgac tggatggttt
     2281 tgaagaacct gctcatcgaa ggcaaggcgg agacccgtcc cggcttcaac ctggagtatt
     2341 gcatgcgcat gggcactgct tatttcgcct ggttcttcga gggtgtgtat cagcagatta
     2401 acaaggaagg tctgtacaaa gagtaacatg gccgagtctg atcaagagga aatgtcaccg
     2461 cgtccgttga tgcggatact cggcgatttc gccaatagcc aaatcttgga cgcggcgctg
     2521 gagtacgatt ttttcacgct gatcagtaaa ggtttcgata gcgccgaagc gttggcgcgc
     2581 gaagccggca cggacgcgcg cgccacgcgc attgtgctcg acagcttgcc ggcgctgtct
     2641 ctgatcgaaa agcgtgacgg aaagtatttc ctcacaccgt ccgcgcaggc ctttctggtc
     2701 aaaggcaggc catcctattg cggcgatttc cggcatgtcg ccctggcgtt gtgggatggc
     2761 atggcgcatc tcaaagaatc gctcaaaacc ggtaagcctc tcagccgcat ggataccggc
     2821 accgaactgc cggtttggga aaaactcgtg cttggtatta tcgtcatcgc cgagccggcg
     2881 gccaaagcgc tatgcgatat cctaaaaatc ggcagtgagc gcaaaggctt gcgcgtgctc
     2941 gatgtggccg gcggttcgag catcttcggc atgaccatcg tcagccgcga cccgaccgca
     3001 caggtcacgc aactcgactg gcccaacgtc aacgcggtgg cgcagaaggc gaataaagag
     3061 cgcggcttgg agggcaagat tcgttttatc gacggcgaac atcataccgc gaacatcgaa
     3121 accaatcact atgatctagt gctcgccagc aacttctgcc gcttcgaatc gcccaagggc
     3181 aatcaggaat tatttgctaa agctttttcc gcgttgaagg ccggcggaat tttcgtcgtc
     3241 aacgacttcg tgcccaacga agaacgcact gagcctacat ttgcgttgcg tttctcggtc
     3301 tatacgctga cccatacacc ggcaggcgag tgctggacgt tggcgcaata ttccgactgg
     3361 atgaagaaag cgggattcaa ttcaatcgcc acccacggcg atatttccaa gactctgccg
     3421 ggaaccacgc tgattttggc aaccaagagt tgagaattaa gccaagcctg tcggctgggc
     3481 tcgggcaacc ggcatgcaat gctggcaaag gattatcaac tagattttct atgccggttt
     3541 cttcccgctg ctttctttcc cagcggctga atgcgtcgcc tgcgacggga cctttgaacg
     3601 ggtgctgggc caagcgccga gttattttcc acctgagcgc aatgttcgct gctgacactg
     3661 acgccgattc tcggcgcggc ggtcgcgttc cagcgcgctg aattttccta ggctaaagcc
     3721 tctttcgtcg ctatttatca cgccttctgc gctcactttc tttcgataat tcatcatctt
     3781 atggccgcgg cagccatgga tcaatagttt gcctagcttt gcccaatgct agatgctaga
     3841 gtccggcggt gccgatgaaa cgccaagccg cctgttgcgc cgtgcaattc cgccgcctct
     3901 agatcccgct cgcgggatat cgttatgcaa tatctggcta tctgcgagga cgatctgtta
     3961 tttcagttta tgcgcaatgt cgccaccgag cagggcgatg tgcgctatct cgtgcccaac
     4021 gcaaaaatcg cccagcgcat cagacggcaa cggggcaaag tttggcatgg cgatctcctc
     4081 aaagaagaga cctacaagaa aaccaaattg catcatgtcg atcaggcgat cgtcttcgtc
     4141 cgtgatgccg actaccaaca tcgcgtctgc cggttgttgc gggtcctcga caaaaacatt
     4201 ccgatcgttt cgttgacctc ggaaaaaaac ggcaaaaagc ccgcgaaaga acctgacgat
     4261 ccgctgttac gtcacttaca gttagccgat atcttcgcgg agttttgcgc cacgcagttg
     4321 ctcgacaccc tcaatatcaa gaaagtcgag cgcatccgct cgctattcaa cgacggcgaa
     4381 aagattcaca tattattgca gcatgatccc gaccctgacg ccatcggcag cgctttggcg
     4441 ttgcgcgagt tactcggtcg caaccgcacc accacaccga tcgtgacgtt cggtaccgta
     4501 acccggccgg agaatttggc gatgatcgag ctgttggaaa ttcaaatcga gcaaatcact
     4561 cccgatgagt tataccaaga cggcgcgcgc ctagctttgg tcgatgtcca gccgccttac
     4621 tttgagcagc ccctgcgccg ggtcgacctg gtcgtcgacc atcatccgaa acggagcaag
     4681 ttcaaggcgc gcttcgccga tttgcgcacc ggctatggtt cgacggcgac gatctttaca
     4741 gaatatttac gtgccgctgg catggagccg tcgcagcgct tagccaccgc gctgctctac
     4801 ggcatcaaga ccgataccct gtttctcgag cgcggcagta atctcgccga cttgagtgcc
     4861 ttcaactacc tttacccgat cgccaacaaa acaataatcg cgcgcatcga acggccggcc
     4921 ctaccgcggg aagatctgga agcgatgggc cgggcattga cccacctgca agtcgacagc
     4981 tccgtcgccg tgatccactt gggcgaagtc aatcgcgaag acgtgattcc ccagatggcg
     5041 gagttctgtt tgcaaatcga aggtgttgac tggggggtcg tatcgggttt ggtgaacaat
     5101 cgtgtggtca tctcggtccg caacgtgggt tatgtcaaga gcgccggcga gatcatgaaa
     5161 aaactctacg acgacatcgg cagcgccggc ggtcaccgcg ccatggctaa agcggtggtt
     5221 cccctcgacc gtttcaaaga gcgcttcggt gaagtcagcg agaaagtcat tcgtgaagcg
     5281 atgctgccgc tgatcgtcga caaagatcag atcgagctcg aactaaggtg agtcgagcag
     5341 aactagagtg atggggtttc cgcaatcccg ccgcttcgat actccgtcct gactccattt
     5401 cagccgttgc ccaaacttca attgacaagc caacccgcgc cgcgataggt ttgatccact
     5461 gaaaaacctg gttcgggagg gggcgttatg gaatgcatca atctggggca gaagatcgaa
     5521 gcagcgcgcg cgggcaaaat caaaaataac catttttttg gcacggataa ctttcgctcg
     5581 tggatgcttt acttcgagcc aggcgacgat acaccgatgc attttcatca gaaccctgag
     5641 acgtttctgg tggtgcaggg ggaatgctcg attaaagatt ttaaaggcgg agagcgggta
     5701 gcgaagaaag atgacatcgt cttttttccg gccaaggaat attatcaatt aatcaataag
     5761 ggaaccgagc cgctggtaat gttcggtaac cgttccgaac cgttcggcat cggcatcacc
     5821 cgcgctgaag ccgcgaagta aaccctacgt tcgcaacctt ttcccaggag aattacatgc
     5881 ccaaactgcg tcatctggcg attaaaacgg aagataccgg caagctcgcg acgttttaca
     5941 tggacgtttt cgagatgcag gttttgcaca aagacagaaa agaaggcggc gcagtttatt
     6001 tgaccgacgg ctatttcaat ctcgcgatcc tgccgaacaa tgaacaacag tcacccaacg
     6061 gactgtacca tttcggtttc gaagtggaga acggcgagaa gatcgtcgaa cggctaaaaa
     6121 agttcaatcc caacaaaatg ccgaaacagc gccccaacgg ccggccctat gccgaaacgc
     6181 gcacttcgga tatcgacggc aactttttcg atatttccga acacggcttt caagtcgttc
     6241 aaccggtggg cggcaaggaa gagaattaac agtacctgag aatcatcaag gcaaggagga
     6301 ccatatgcct aagatcaaac atttggccat tcgcacggaa gataccggca agctcgcggg
     6361 cttctatatg gacgtcttcg agatgcagat gatacacaag gaaaaagacg agggcggcgc
     6421 gattttcctg accgatggct atttcaatct agcgatcctg cccaatcatg aacagaacgc
     6481 gcccaacggg ctgtaccatt tcggcttcga agtggagagc ggtgagaaga tcgtcgaccg
     6541 aatgaaaaag gtcaacccca ataagttgcc gaaaagccgt cccaacggcc gtccctacgc
     6601 cgagactcgg ggttccgatc ccgatggcaa ttatttcgac atctccgagc atggctttca
     6661 aaatgtcgtg cccctgggcg agaaaaagag cgactagtca gccgtagcga tagccgcttc
     6721 tcgtatcctc gattgttcgg cgagggcgat acgtcgccga cccaagaacg aacaaacgga
     6781 gtcagcgcga tgacgagaat cactttgcct cacggccgcc gacggttgcc tggcgcgtgt
     6841 ggtatgttcg ttaccctctc gctggttttt tgccttccgg gtacttcgag cggtcagcct
     6901 aaatcaaatc tgtcgtccag cgtgaccagc gaaagcatga ctcatgtctg ggtagcgcgg
     6961 gagcgcggct tgttcaagaa atatggcgtc gacatgcagt tcgtactcat gccgcgcaat
     7021 cccttggcca tcgcggcttt gcttgcggga gaaattgacg cggcgatcat cggtcctggc
     7081 catctcgtca atgcgggtct gagcggcgcg gagctcgcag gcatcgccaa cttcaatcag
     7141 aaattggatt ttcgcctaaa cacgcgcccc gacatcaaga agccggagga gcttcgtggt
     7201 aaacggatcg cggtcagcgg tccgggatca acgtcgcatc tggtgtcgat gttggcgctt
     7261 caaggactca atctcgaccc cgttcaagcg aagatttcct ttctgacgat accggggaca
     7321 gaaatgaacc ggcgcttggc ccttgagagc ggcagtgtcg atgccaccac actaaggggc
     7381 gccatgggcg aggtctacgc caacaaaggg taccccttgc tttataactt gaattccgcc
     7441 ggcgtgaccc tgccgcagaa catgatggta attacgcggc gagcggcggc gacaaagccg
     7501 caggtgatcg aaggccattt gaaagccacg atcgaggcga tcgcgatgat taacgatccg
     7561 gcgaatcggg aactggtgag ccggctcttg gcaactaacc tgcgtttgag caatccggcc
     7621 gatatcgaag aatcctatca aaccgtgatc accaactacg agcgcgcgcc gcaccccagc
     7681 gtggagggaa tgaagcggct gcaaaaactg ttaatccaac ttaacccgaa gatcgccgat
     7741 gtgcgcgtcg agacgcttat cgacaactcg ttcatgaata agctcgaaag ttccgggttc
     7801 attcagggct tgtataaaaa gaattgataa atcactcgtt atagtaatcg tcatcgaaga
     7861 aggagaattt gcaccgtgag aaccgaaaac attcccgtag tcgttgtcgg cggcggcagc
     7921 gcagcctttg aagcggcggt ggccgccaag cagtccggcg cagcgaaagt cgtgatgctg
     7981 gaaaaagcgc cggagccgga gtttggcggc aatgcgcgct attcgcacac cggcttccgc
     8041 tgggtatttt ccggcgccga tgagatccgg cgattcttgc cggaggtcga cgacgcgatg
     8101 tttgcgaaac ttaacttgcc ggcctattcc agcgagttgt ttcacgccga tttgcagcgg
     8161 gttacgcgcg gccgcatcga ccagactcta gccgacgtgc tggtgagcca gtcaaacgcg
     8221 gcgat
//
LOCUS       DASQKS010000705         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5856696, whole genome shotgun sequence.
ACCESSION   DASQKS010000705 DASQKS010000000
VERSION     DASQKS010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5856696"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VE170_18275"
     CDS             complement(<1..624)
                     /locus_tag="VE170_18275"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HYJ17448.1"
                     /translation="MHKPAPLAPGARIGVVAPAGCVASAALLAGVAAIEAQGYVVELA
                     PGIYQSNGYLAGAAADRARDLRDFFRRGDIAAIFCARGGFGSIQTLEYLSIDLEKYPK
                     LFVGYSDITILLDWLRRRCSMVTFHGPMVATDLARGLSDRSGEHFWGMLDGSKRGWRL
                     NLGETLRRGQAEAEMTGGCLSMLVTTLATAYEIDSRGKILFLEDVGEA"
     gene            912..1187
                     /locus_tag="VE170_18280"
     CDS             912..1187
                     /locus_tag="VE170_18280"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HYJ17449.1"
                     /translation="MKIKPEQLDRLAAQLIASYRKKDLIVSKASDAQLTAKVIEAINR
                     NFSEEEAIEEEARKMLASFAQASKDMDPFKMFLLAKQKLAAKKGFIL"
     gene            1184..1459
                     /locus_tag="VE170_18285"
     CDS             1184..1459
                     /locus_tag="VE170_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HYJ17450.1"
                     /translation="MSRLSESRISHLAHLVLESLRTAKLAEFPSEGRALTETKLVLQE
                     FFNFEDQVDDAVRQKIASLSRHVPPGSREWDVLYRKYHEEETRKHRR"
     gene            1644..1934
                     /gene="groES"
                     /locus_tag="VE170_18290"
     CDS             1644..1934
                     /gene="groES"
                     /locus_tag="VE170_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HYJ17451.1"
                     /translation="MKIRPLQDRVIVKRIAEEEKTKGGIIIPDTAKEKPQEGKVIAVG
                     KGKTNDDGKITPLDVKVNDRILFGKYSGSEIFVDNEEHLIMREDDILGIIEK"
     gene            1963..>3519
                     /gene="groL"
                     /locus_tag="VE170_18295"
     CDS             1963..>3519
                     /gene="groL"
                     /locus_tag="VE170_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYJ17452.1"
                     /translation="MAAKIVKFNRDARDAILRGVNTLADAVTVTLGPKGRNVVLDKSF
                     GAPTVTKDGVTVAKEVELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARQIFAE
                     GVKLVAAGHDPMTLKRGIDKAVTAIIGELRTLSKPTKDPKEIAQVGTIAANNDSTIGA
                     VISEAMNKVGKEGVITVEEAKGLETTLNVVEGMQFDRGYLSPYFVTDPERMECVLEDA
                     YLLIHEKKISTMKDILPILEQIAKSGKPFIIIAEDIEGEALATLVVNKIRGTLRCVAV
                     KAPGFGDRRKAMLEDIATLTGGKLIAEELGIKLDNIALTDLGRAKRIVVDKDNTTIID
                     GAGKKSDLEGRIKQIRAQTDETTSDYDREKLQERLAKLIGGVAVINVGAATEIEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRSSVVLEKLRVSEEERWGVNIVKRVCEEPL
                     RRIAINAGVEGSIALQKVKDGKGAFGFNAATEEYEDLLKAGVIDSAKVVRTALQNAAS
                     VASMLLTTEAM"
BASE COUNT          883 a         1022 c          963 g          651 t
ORIGIN      
        1 cgcttcgccg acatcttcga gaaagaggat tttgccgcgg ctgtcaatct cgtaggcggt
       61 tgccagcgtg gtcacaagca tcgataaaca accgccggtc atctccgcct cggcctggcc
      121 gcgccgcaga gtttcaccca aattcaatct ccagccgcgc ttgctgccgt ctaacatacc
      181 ccagaaatgt tccccgctgc gatcgctcaa gccgcgcgcc aggtcggtgg ccaccatcgg
      241 accgtggaac gttaccatgc tgcagcggcg ccgcagccag tcgagcagta tcgtaatatc
      301 gctgtatccg acgaagagtt ttggatattt ttccagatct atactcaaat attccagtgt
      361 ttggatcgaa ccgaaaccac cgcgggcaca gaagatcgcg gcaatatcgc cgcggcgaaa
      421 aaaatcgcgc aagtctcgtg cccgatcggc ggcggcgccg gccagatagc cgttgctttg
      481 gtagatgcca ggcgccagct cgactacata gccctgggct tcgatggcgg caacgccggc
      541 gagcagcgcc gccgacgcca cgcagccggc cggagcgacc acgccaatgc gcgcccccgg
      601 cgccagtggc gcaggtttat gcacagccaa ggaagttttc cacaacctga gtggatagat
      661 cgtctgacaa gcctgcttcc tcctgtggat atcttgcgaa tgggttgcgg acgttagcca
      721 ttgcggttgc ttatcttcgc aaaaaagccg cccgtgcaca acaatttagc cgcgccgatc
      781 ggcgctcggc gggcaattgc cgctcggtaa ttgacatcaa atcgcaggct cgataaaact
      841 ccagagggtc agacatgata ccgggtaagt caccggttca cgacaggttc cccacagatt
      901 attcacagcc tatgaagatc aaacccgaac agctggatcg tctggccgcg cagctcatcg
      961 ccagctatcg aaaaaaagac ttgatagtca gtaaagctag cgacgcccaa ctcacggcca
     1021 aagtcatcga agcgatcaac cgcaacttca gcgaagagga agcgatcgag gaagaagccc
     1081 ggaagatgtt ggccagcttc gcccaggcca gcaaagacat ggatccattc aagatgtttt
     1141 tgctcgccaa acagaagctc gcggccaaga aaggcttcat cctatgagcc gactatcgga
     1201 gagtcgcatt tctcacttag cccatctcgt gctcgagagc ttgcgcacgg cgaaattggc
     1261 cgaattcccg agcgaaggcc gtgccctcac ggagaccaag ctcgtgctcc aggaattttt
     1321 taattttgag gatcaggtcg acgatgcggt gcgtcagaag atcgcatccc tgtcgcgcca
     1381 tgtgccgccc ggcagccgcg agtgggatgt gctgtaccgc aagtaccatg aggaggagac
     1441 gcgcaagcat cgccggtagt atacatttcg aacccgcttg atatttatga gaatttcccc
     1501 aattaatacc ttgacagctc aagattgatg cttagattca gcccgctcta gttcgtcccc
     1561 ggccctgcgc cggcggcttc aattagcggc aaattacttt tagatcagtc agttaaactc
     1621 aaccgcttga aaggaggcgc gcagtgaaaa taaggccttt acaggaccgg gttattgtca
     1681 aacgcatcgc cgaggaggaa aaaaccaagg gtggtatcat tatccccgac accgctaaag
     1741 aaaagcccca ggaaggaaaa gtcatcgccg tcggcaaagg taaaacgaac gacgacggca
     1801 agatcacccc tctcgatgtc aaagtgaatg accgcatcct atttggaaaa tactccggct
     1861 cggaaatttt cgtcgacaat gaagagcatc tgatcatgcg cgaggacgac atcctcggca
     1921 ttatcgaaaa ataattaaac agataatctg aggaggaaaa cgatggcagc taaaatagtc
     1981 aaatttaatc gcgatgcgcg tgacgcgatc ttaagaggcg tgaacactct cgccgacgcg
     2041 gtcacggtga ccttgggccc gaaaggccgc aatgtcgtat tggataaatc cttcggcgcg
     2101 ccgactgtga ccaaggacgg cgttaccgtc gccaaagagg tcgagctcga agacaagttc
     2161 gaaaacatgg gcgcacaaat ggtcaaagag gtcgccagta agacctccga cgtggccggc
     2221 gacggcacga caacggcgac cgttctggcc cggcagatct tcgccgaagg cgtcaaattg
     2281 gtggccgccg gccatgatcc tatgaccctc aaacgtggca tcgacaaggc cgtgaccgcc
     2341 attatcggcg aacttagaac cctatcgaaa ccgaccaagg accccaagga aatcgcccag
     2401 gttggcacca tcgctgctaa caacgactcg accatcggtg cggtcatctc cgaggcgatg
     2461 aacaaagtcg gcaaggaagg cgtcatcacc gtcgaagagg ccaagggctt ggagacaacc
     2521 ctcaacgtag tcgaaggcat gcagttcgac cgcggctatc tctcccctta tttcgttacc
     2581 gatccggagc gaatggaatg cgtcctcgaa gacgcctatt tgctcatcca tgagaagaaa
     2641 atcagcacga tgaaagacat tctgccgatt ctcgagcaga tcgccaagtc cggcaagcct
     2701 ttcatcatca tcgccgaaga catcgaaggc gaagccctgg cgactctggt ggtcaacaag
     2761 attcgcggca ccctgcgctg tgtcgccgtc aaggcccccg gcttcggcga ccgtcgcaaa
     2821 gcgatgctcg aagatatcgc caccctcacc ggcggcaagt tgatcgccga agaattaggc
     2881 atcaagctcg acaatattgc cttgaccgac ttgggtcgcg ccaagcgtat cgtcgtcgac
     2941 aaggacaaca cgaccatcat cgacggcgcc ggcaagaagt ccgatctcga aggacggatc
     3001 aaacagattc gcgcccaaac ggatgagacc acttccgact acgaccgcga gaagcttcaa
     3061 gagcgcttgg ccaagttgat cggcggcgtc gcggtaatca acgtcggtgc ggccaccgaa
     3121 atcgagatga aagaaaagaa ggcccgcgtc gaagacgcct tgcacgccac ccgcgctgcg
     3181 gtcgaagaag gcatcgtgcc cggcggcggc gttgcattgc tgcgctcctc ggtcgtgctg
     3241 gaaaaactgc gcgtctccga agaagagcgc tggggggtca atatcgtcaa gcgcgtctgt
     3301 gaggaaccgc tgcgccgcat tgccatcaat gccggtgtcg agggctccat cgcgctgcaa
     3361 aaggtcaaag acggcaaggg cgccttcggc ttcaatgccg cgaccgaaga atatgaagac
     3421 ctgctcaagg ccggcgtcat cgattcggct aaagtcgtcc gtacggcact ccaaaacgcc
     3481 gcttcggtcg caagcatgtt gttgaccacc gaagcgatg
//
LOCUS       DASQKS010000706         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5458305, whole genome shotgun sequence.
ACCESSION   DASQKS010000706 DASQKS010000000
VERSION     DASQKS010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5458305"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3254
                     /gene="uvrA"
                     /locus_tag="VE170_18300"
     CDS             <1..>3254
                     /gene="uvrA"
                     /locus_tag="VE170_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYJ17453.1"
                     /translation="NQLTVVTGVSGSGKSTLAFDILYAEGQRRYVESFSAYARQFLDR
                     RDKPDVESIEGIPPTIAIDQSRPVRTSRSTVGTMTELHDHFKLLFAKIAVLYCRGCQR
                     IVERDSAVTVLQKLGDLAEGTPVVLTFPLPASSVSWDEVRNGLRQAGFHRMLIDRTIC
                     ELDDVDKAPAKKQPLEVVVDRFAFRVENQKRIADSLEQAFHYGKGRLNLFLPQQDWRQ
                     EPFSNHLHCPHCDISYRDPVPNIFSFNSPLGACETCKGFGRVIDIDIDLVIPNPGKSL
                     GDGAIKPWLNRKRRTKRLLDFCNGQKIPVKTPWGDLSDAQRQLVIDGDGEYKGIRGWF
                     RRLERRSYRMHVRVLLARYRAYLLCPECNGSRLKADALNYRIDGKDIAEINAMNVGVA
                     NEFFGALKPAGALDQVSSLILGEIRRRTGYLVGVGLEYLTLDRQSRSLSGGELERVDL
                     TTAIGSSLVNTLYVLDEPSIGLHPRDSRRLVEILQRLRANQNTVVIVEHDPEIIKECD
                     YILDLGPKAGEQGGEIMFAGTYDELLKSETSLTAAYLSQRKSIAIPARPRKRLLHRSL
                     KITGASANNLKNIDVEIPLGMLVCITGVSGSGKSSLVDEVLSRNLKKLKEAPSANFAN
                     CEKIDGIEKISEVILVDQSPVGTTPRSNPATYMKAFDGIRKLFAGAELARLRGYTPST
                     FSFNVEGGRCETCRGEGYEKVEMQFLSDVYTTCAECHGSRFREEVLEVAYRGKNIRQV
                     LDLTVSDALEFFKESNDVKASLEPLRAVGLEYLRLGQPVTTLSGGESQRLKLAAHMAR
                     AKKAGTLFIFDEPTTGLHFHDIDRLLWAFNELIEQGHSVVVIEHNLEVIKCADHIIDL
                     GPEGGDKGGEIVATGTPAQIAAVARSYTGVYLRPYLNKSTSLFAPVLKNDAGPRRVLP
                     AGADDNAIAIVGAKEHNLKNISVNIPRDRFIVFTGLSGSGKSTLAFDIVYAEGQRRYL
                     DSLSAYARQFLEVMARPNVDYVAGIPPTVAIEQRLSQGGRNSTVATVTEIYHYLRLLY
                     AKIGKQHCVNCGRPIHKLTRSQILDRISRSYRGKDVMVLSPLVRGRKGFHKEVI"
BASE COUNT          694 a         1047 c          889 g          624 t
ORIGIN      
        1 caaccaactc accgtggtca ccggggtcag cggctcgggt aaatccaccc tcgccttcga
       61 tattctctac gccgaaggcc agcgccgcta tgtcgaaagc ttttcagcct acgcccggca
      121 attcctcgat cgcagggaca agccggacgt cgagagcatc gaaggcatcc cgccgaccat
      181 cgccatcgat cagagccggc cggtgcgcac ctcgcgctcc accgtcggca ccatgaccga
      241 gctgcacgat cattttaaac tattatttgc caagatcgcc gtgctctact gccgcggttg
      301 tcagcgaatc gtcgagcgcg acagcgcagt tacggtcctg caaaaactcg gcgacctggc
      361 cgaaggcaca ccggtggtct tgacctttcc cctgccggcg tcgtcggtga gttgggacga
      421 ggtccgcaac ggtctgcgcc aggcgggctt ccatcgcatg ctgatcgacc gcaccatttg
      481 cgaactcgat gacgtcgaca aagcgccggc aaaaaaacag cccctagagg ttgtcgtcga
      541 ccgcttcgcc tttcgtgtag agaatcaaaa gcgcatcgcc gactctttgg agcaggcgtt
      601 tcactatggc aaaggccgct tgaatctatt cctgccgcag caggattggc gccaagagcc
      661 gttcagcaat catttgcatt gcccgcactg cgatatttcc taccgcgatc cggtgccgaa
      721 catattttcg ttcaatagcc cattgggtgc ctgtgaaact tgcaaaggct tcggccgagt
      781 catcgatatc gatatcgacc tagtcattcc caatccgggc aaatccctcg gcgacggcgc
      841 catcaaaccc tggctcaacc gcaagcgcag aaccaaacga ctgctcgact tttgtaacgg
      901 ccagaaaatt ccggtgaaaa cgccttgggg ggatctaagc gacgcgcagc gtcagctggt
      961 catcgacggc gacggtgaat acaaaggcat ccgcggctgg tttcgccgcc tcgaacggcg
     1021 cagctaccgc atgcatgtgc gcgtcttgct ggcgcgctac cgcgcctatt tgctctgccc
     1081 cgagtgtaac ggcagccggc tcaaagccga cgcgctcaac tatcgcatcg acggcaaaga
     1141 tattgccgag ataaacgcca tgaatgtcgg cgtcgccaat gaattttttg gcgcattgaa
     1201 accggccggc gccctcgatc aagtgtcgag tctgatcctc ggcgaaattc gccgccgcac
     1261 cggctacctg gtcggtgtcg gcctggagta tctcaccctc gaccgccaat cgcgctcact
     1321 ctccggcggt gagttagagc gcgtcgactt gaccacggcg atcggctcgt ccttggtcaa
     1381 cacactctac gtcctcgacg aaccgtccat cggtctccat ccgcgcgaca gccgccggtt
     1441 ggtggaaatc ctccagcgct tgcgggcaaa tcaaaatacc gtggtcatcg tcgaacatga
     1501 tcccgagatc atcaaggaat gcgattatat attggacctc ggccccaagg ccggcgagca
     1561 aggcggcgaa atcatgttcg ccggcaccta tgacgagcta ttgaagagcg aaacctcgct
     1621 caccgccgct tatctaagtc agcgcaaatc gattgccatt cccgcgcggc cgcgcaagcg
     1681 gctgttgcat cgctcactca agatcaccgg cgcctcagcg aacaatctaa aaaacatcga
     1741 cgtggagatt ccgctcggca tgttggtgtg catcaccggc gtctccggct cgggaaaatc
     1801 gagtctggtc gatgaagtgc tgtcgcgcaa tctgaaaaaa ctcaaggaag cgcccagcgc
     1861 caacttcgcc aattgtgaga agatcgacgg catcgagaaa atctccgaag tcatcctggt
     1921 cgatcaatcg ccggtcggca ccacgccgcg ctcgaacccg gcgacttaca tgaaagcctt
     1981 cgacggcatc cgcaagctgt tcgccggcgc cgagctggcg cggctgcgcg gctacacgcc
     2041 atcgacattt tctttcaacg tcgagggcgg ccgctgcgaa acctgccgtg gcgaaggcta
     2101 cgaaaaagtc gagatgcagt ttctctccga cgtctatacc acgtgcgcgg agtgtcacgg
     2161 cagccgcttt cgcgaagaag tcctcgaagt cgcctaccgc ggcaaaaaca ttcgccaggt
     2221 gctcgatctc accgtcagcg acgccttgga gtttttcaag gaaagcaatg atgtgaaggc
     2281 atctctcgaa ccgctgcgcg ccgtcgggtt ggaatacctg cgcctgggcc agccggtgac
     2341 gacgctctcc ggcggcgagt cgcagcgcct gaagctcgcc gcccatatgg cgcgggcgaa
     2401 gaaagccggc accctgttta ttttcgacga gcccaccact gggctgcact tccacgacat
     2461 cgaccggctg ctctgggcgt tcaacgaact gatagagcag ggccattcag tggtcgtcat
     2521 cgagcataac ttggaagtca tcaagtgtgc cgaccacatt atcgatctcg gcccggaggg
     2581 cggcgacaaa ggcggcgaga tcgtcgccac cggcacgccc gcccagatcg ctgcggtggc
     2641 ccgctcctat accggcgtct acctgcgccc ctatttaaac aaatcgacat cgctgtttgc
     2701 gccggtgtta aaaaatgatg ctggcccaag gcgtgttttg cccgccggcg ccgacgacaa
     2761 cgccatcgcc atcgtcggcg ctaaggagca taacttaaaa aatatcagcg tgaacattcc
     2821 gcgcgaccgc ttcatcgtct tcaccggcct cagcggttca ggaaagtcga ccctggcctt
     2881 cgacatcgtt tacgccgaag gccagcgccg ctatctcgac agcctgtccg cttacgcgcg
     2941 gcagtttttg gaagtgatgg cgcgtcccaa cgtcgattac gtcgccggca tcccgcccac
     3001 cgtcgccatc gagcagcgct tgagccaggg cggccgaaac tccaccgtcg ccacagtcac
     3061 ggaaatctat cactacctgc gcctgctcta cgccaagatc ggcaaacagc actgcgtcaa
     3121 ctgcggccgg ccgatccata agctgacccg cagccagatc ctcgaccgca tctcgcgctc
     3181 ctaccgcggc aaagatgtca tggtgctaag cccgctcgtg cgtggccgta agggatttca
     3241 caaggaagtg atca
//
LOCUS       DASQKS010000707         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5459817, whole genome shotgun sequence.
ACCESSION   DASQKS010000707 DASQKS010000000
VERSION     DASQKS010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5459817"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..477
                     /gene="ccmE"
                     /locus_tag="VE170_18305"
     CDS             34..477
                     /gene="ccmE"
                     /locus_tag="VE170_18305"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HYJ17454.1"
                     /translation="MTKGKRFLIGGVVILAALAYIIYGGMQQALVYFKMPSELRAEES
                     AMKGKFVRMGGMVVKGSMEKDLQNLTYKFHITDGGTSFPVYFKGVPPDLFVEGKGAVV
                     EGRVENDGVFHATTIMAKHSEDYSPHADGTSASKSFVPAREAGAK"
     gene            474..2435
                     /locus_tag="VE170_18310"
     CDS             474..2435
                     /locus_tag="VE170_18310"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HYJ17455.1"
                     /translation="MTASIGHSSIVIAFIAALIGILSPLVAARSAGSRFRAVASYAIL
                     GQFIFVTLAAVALIYGLVSTDFSIKYVAFNTTRATPVYYRVTGLWGALEGSLLLWEWI
                     LIIFAGIVAWVYRQRHHEMMPWVIMIFSIVSAFFLGVIGFLSNPFETVFPIPLDGRGL
                     NPLLEDANMMTHPPLLYTGFVGLTVPFAFAMAALIVGKLDDAWVVSTRRWTITAWFFL
                     TAGNLVGAWWSYHVLGWGGYWAWDPVENAAFMPWLPATAFLHSIQVQERRRMLKVWNL
                     ALIIVAFSLTIFGTFLTRSGIISSIHAFSSGPVGGFFLGFLAFILLGSFGLLAYRADL
                     LKEQPELDSMVSRESAFLLNNIVLVSALFTIFLGTIFPLISEAVAGTQVSVGAPYFNS
                     VTVPLFLVLVFLMGVGPMIAWRRASWDNLRRNFLWPAAISLSFGLLLFVWKVKDFLPL
                     LGFTLLAFVVSSILYDTALALRTRKRIAGEGILRGLISLTRRNQRRYGGLVVHLGVVL
                     IMMGIAGSMTYSSEKDATLALNQTLDIGNYRVRFEGLQGSQQPTHFRVEGAFRVFHNG
                     SDEGIVNPALKFFPTQQSPIGRAVHQSSLSEDIYLILSGFSEVNRNQATLKVLVRPLV
                     IWMWIGGFVIAFGTIICLAPMGRLKPQAD"
     gene            2464..3030
                     /locus_tag="VE170_18315"
     CDS             2464..3030
                     /locus_tag="VE170_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ17456.1"
                     /translation="MPVRRLIITLLIIAPILALLAYGFTRDAKYIKSPLLAKPATPFS
                     VTLYDGKKVTLEDFRGKAVFLNFWASWCTPCRAEARDLEAAWQKVKDKNMIFLGVALQ
                     DLDDNAKTFLQEFNVSYPNGRDESGKIAVDYGTWGIPESFFIDPQGRITYKHVGAIRA
                     ALVLSKLDEAAKGIASAQEGRGDYEGAR"
     gene            3036..>3971
                     /locus_tag="VE170_18320"
     CDS             3036..>3971
                     /locus_tag="VE170_18320"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HYJ17457.1"
                     /translation="MRYIKLWLVYVFLAAFIQTLHAASNDEIEDRTREIATELRCVVC
                     QNLSVADSPSEMAQQMRAIVREQVQAGKSPEQIKEFFVSKYGDWVLLRPKTTGVSALL
                     WILPYVVLVCGILAALWFIRRWTTRKPPAEGPISEGTTANQAQRDALREEFQLPDSAD
                     ETERAQLLRGRSHVRDELNELEFDFQSGKLSAADYTQLRQEIESKGLSMLQQLSLLPE
                     PPKAKPQDMKAKPRQDASAPNRFKRWQLIGGGVFLLLFGLTLGIMLTQSIRPRQGEGD
                     TMTGDFMTGTTPMGGAAGAAMAEGKQAFQRQEFPKA"
BASE COUNT          785 a         1192 c         1088 g          906 t
ORIGIN      
        1 accggttaaa gtcctcggag gcagttaata gccatgacaa aaggtaagag gtttctaatc
       61 ggcggtgtcg ttatactcgc agcgctcgcc tacattattt acggcggcat gcagcaggcg
      121 ctggtctatt tcaaaatgcc gtccgagctc cgcgccgaag aaagcgcgat gaagggcaag
      181 ttcgtgcgca tgggcggcat ggtcgtcaaa ggctccatgg aaaaagatct gcaaaacctc
      241 acctataaat tccatatcac cgacggcggc accagcttcc cggtctattt caaaggcgtg
      301 ccgccggatc tgttcgtcga aggcaaaggc gccgtggtcg agggccgggt cgaaaacgac
      361 ggcgtgtttc acgccaccac gatcatggcc aaacactccg aggattatag cccccatgcc
      421 gacggcacgt cggcaagtaa aagttttgtc cccgccaggg aagccggcgc caagtgaccg
      481 ctagcatcgg tcatagctcg atcgtcatcg ctttcatcgc ggctctgata ggtatcctga
      541 gtccgttggt ggccgcgcgt tcggcgggaa gtcgctttcg cgcggtggcg agctacgcga
      601 tcttgggcca attcattttc gtcaccttgg ccgcggtggc cctgatctat ggcttggtca
      661 gtaccgactt ctcgatcaag tacgtcgcct tcaacaccac ccgcgccacg ccggtttact
      721 atcgtgtcac tggactatgg ggcgcccttg aaggctcgct cttgctctgg gaatggatac
      781 tgatcatctt cgccggtatc gtcgcttggg tctaccgcca gcgccatcat gagatgatgc
      841 cctgggtgat catgatcttt tccatcgtct cggcgttctt tctcggcgtc attggttttt
      901 tgtccaatcc gtttgaaacc gtatttccga ttcccctcga cggtcgcggt ttgaacccgc
      961 tgctcgaaga cgccaacatg atgactcacc caccgctgct ttacaccggg ttcgtcggct
     1021 tgacggtgcc cttcgccttt gccatggcgg cgttgatcgt cggcaagctg gacgatgctt
     1081 gggtggtcag cacccgtcgc tggacgatca ccgcgtggtt ttttttgacc gcgggcaatc
     1141 tcgtcggcgc ctggtggtcc tatcatgtgc tcggctgggg cggttattgg gcctgggacc
     1201 cagtggaaaa cgccgccttc atgccctggc tgccggccac cgcttttttg cattcgatcc
     1261 aagttcaaga gcgccggcgc atgctcaaag tgtggaatct ggcgctgatt atcgtcgcct
     1321 tcagcttgac catcttcggc acctttctca cccgctcggg cattatctca tcgattcatg
     1381 ctttttcatc gggcccggtt ggcggtttct ttctgggctt tctcgccttc atcttattgg
     1441 gctccttcgg cctactcgcc taccgcgccg atctcttgaa agaacagccc gagctcgact
     1501 ccatggtcag ccgcgagtcg gccttcttgc tcaacaacat cgtgttggtt tccgccttgt
     1561 tcacgatttt tctcggcacg atctttccgt tgatctccga agccgtcgcc ggcacccagg
     1621 tgagcgtcgg cgcgccttat ttcaactcgg tcaccgtgcc gttgtttctt gtgttggttt
     1681 ttttgatggg ggtcgggccg atgatcgcct ggcggcgcgc ctcctgggat aatctacgac
     1741 ggaactttct ctggcccgcc gcgatctcgc taagtttcgg tttgttgcta tttgtttgga
     1801 aggtgaaaga ctttctgccg ctattgggct ttaccctgct cgccttcgtc gtgtcctcga
     1861 tcctttatga caccgccctc gcgctgcgca cacgcaagcg catcgccggc gaaggcatac
     1921 tgcgcggtct gatttccctg acccggcgca accaaagacg ctacggcggc ctggtggtcc
     1981 atctcggcgt ggtcttgatc atgatgggta tcgccggctc gatgacctac agcagtgaaa
     2041 aagacgccac cctcgcgctc aatcaaactc tcgacatcgg taattaccgg gtccgattcg
     2101 agggtttgca gggatctcag caaccgacgc atttccgcgt cgagggggcg tttcgggtgt
     2161 ttcacaatgg cagcgacgaa ggcatcgtta acccggcgct aaaattcttt ccaacccagc
     2221 agtcacccat cggccgcgcg gtccatcaga gcagcttgag cgaagatatt tatttaatcc
     2281 tctccggctt tagtgaagtg aaccgcaacc aagccacgct caaagtcctg gtccgcccgc
     2341 tggtgatttg gatgtggatc ggcggtttcg tcattgcctt cggcacaatc atctgcctgg
     2401 cccccatggg cagattgaaa ccgcaagcag actaatattg aattttaccg tgttgaaatt
     2461 ctcatgcccg tacgccgctt aatcataacc ctgctgatca tcgctccgat cctggcgctg
     2521 ctggcctacg gttttacccg cgacgccaag tacatcaagt cgccgctgct ggccaagccc
     2581 gcgacaccgt tctcggtcac gctttatgac ggcaaaaaag tcacgttgga ggattttcgc
     2641 ggcaaggcgg tgtttttaaa tttctgggcg tcgtggtgta cgccgtgccg cgccgaagcc
     2701 cgcgatctcg aagcggcctg gcaaaaagtc aaagacaaga acatgatctt cctcggcgtc
     2761 gccttgcagg atctcgacga caacgccaaa acctttttgc aagaattcaa cgtcagctat
     2821 cccaacggcc gcgacgagtc cggcaagatc gccgtcgact acggcacatg gggcatcccg
     2881 gaaagctttt tcatcgaccc ccagggtcgc attacttaca agcatgtcgg cgctatccgc
     2941 gccgcgctgg tgttgagcaa actcgacgaa gcggccaaag gcatcgccag cgcccaggaa
     3001 ggccgcggcg attatgaggg agcgcgctga caaccatgcg ctatattaag ttgtggctcg
     3061 tctatgtgtt tttagccgct ttcattcaaa ccctccacgc ggcgtccaac gacgaaatcg
     3121 aagaccgcac acgggagatt gcaaccgagc tgcgctgcgt cgtctgtcaa aatctttccg
     3181 tcgccgactc gccgtcggag atggcccagc agatgcgcgc catcgtccgg gagcaagtcc
     3241 aagcgggcaa gagccccgaa cagatcaaag aattcttcgt ctccaagtac ggcgactggg
     3301 tcttgctcag acccaagacc accggtgtca gcgcgctgct gtggatattg ccctacgtcg
     3361 tactcgtttg cggtattctt gccgccctat ggttcattcg ccgctggacg acaagaaaac
     3421 cacccgccga aggtccaatc agcgaaggca cgacagcgaa ccaagcacag cgtgacgcgc
     3481 tgcgagagga attccagttg ccggatagcg cagacgagac cgagcgcgcc cagctgctgc
     3541 gcggacgcag ccatgtgcgc gacgaactga acgagttgga gttcgatttt caatccggca
     3601 aattgtcggc agccgattac acgcaactac gacaggagat tgaaagtaaa ggcctcagca
     3661 tgctgcaaca gctgagtctc ctgcccgaac cgccaaaagc aaaaccgcaa gatatgaaag
     3721 ctaaaccgcg gcaagatgcc agcgcgccaa atcgtttcaa gcgctggcaa ctgatcggcg
     3781 gcggcgtgtt tctcctgctc ttcggtctca cccttggcat tatgctgact caatcgattc
     3841 gcccgcgcca gggcgaaggc gataccatga ccggggattt catgaccggc acgacgccca
     3901 tgggtggtgc ggcaggcgca gccatggccg aaggcaaaca agctttccag cgccaggaat
     3961 ttcccaaggc c
//
LOCUS       DASQKS010000708         4806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5861004, whole genome shotgun sequence.
ACCESSION   DASQKS010000708 DASQKS010000000
VERSION     DASQKS010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5861004"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1182)
                     /locus_tag="VE170_18325"
     CDS             complement(196..1182)
                     /locus_tag="VE170_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYJ17458.1"
                     /translation="MSLGCVLVEEFQPEVLQWLRERVDLVVVNPWVEPQRWDIEAARV
                     DAVISRKGQITREHMEKGGGRLKIVARTGVGVDPSRVDLEAAKELKVWVTNQPGSNAV
                     SVAELAFGQMIALVRHTHAADRAVKDNRWSDYLRFIGTELAGKSLGIVGMGNIGARMA
                     LRARAFEMPFLVYDPYIPESHVTALGGKWIGLSELLSDADFVTIHCPLNRETRDMIGA
                     KELALMKPSAFLINMARGGIVDEAALCRTLEQRKLAGAALDVIAREPPAKDHPLFKLD
                     NVLWTPHLGAVTLEASERGEWGAAQEVIRVLESKRPKNPVVDADEARRRLPA"
     gene            1346..2302
                     /locus_tag="VE170_18330"
     CDS             1346..2302
                     /locus_tag="VE170_18330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17459.1"
                     /translation="MTHKTILSLTLLLALLIPAVNAPAEKVRLALPSRSMGYLPMFIA
                     LHRGFLKDENLELELPALLPNIAHNALLSGEVEYHGVADSALRLAAKGAPLKSIFFSA
                     RLPNYFVMSRPAIKSVSELRGKLIAVSRFGGTTDLAARVALQANGLEPQKDVVLIMIG
                     LGNTRNAALIAGSVDANIANPPDNSMLKQKGFRELLFLGDAIEFPSNGFTTTERRLVE
                     NRDQVKRLLRAFYRGLLFSRENPEESIKIVEREWKLDAATARDSYQSIMKAASRDGAS
                     SEAGLKVHIKLIQNSDKSIGDIALTKIVDFRILEEVRRENPR"
     gene            2313..3281
                     /locus_tag="VE170_18335"
     CDS             2313..3281
                     /locus_tag="VE170_18335"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17460.1"
                     /translation="MKLTIVFVFALLAPSDVYAERVRTVIPRATLNYLSVPVAELKGY
                     FRDENLENETIVIPGSSAIAALVSGEVDYSGAGGTGMRAALRGAPIKAVMFQTEKVTW
                     YLIATPEIAKAGDLRGKRVAVGTVGDTQDSLITLYADRNGVAPRDLIRVAMPSRSTTT
                     TILGLKSGAIHAATVNGDEALIAEKEGLRTLTFLGDLFPYPFQGFMASDKKIAERPGD
                     VKRWLRAMMRALMFIRERPEEAADIAMKKIPMGNPNRPIVIEGIRRFVRALPDGIPGV
                     PSAQGIKNVIEFDVKAPLKIKDEVAPEKFLALKLAGEVREELERKR"
     gene            3317..4297
                     /locus_tag="VE170_18340"
     CDS             3317..4297
                     /locus_tag="VE170_18340"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17461.1"
                     /translation="MVPVLLLLSLLSAQLAWGQEKKNLRVVFVSLAWNAQLPFRVANH
                     RGYFRDQGLTVEQIFVSGGPTAVMALVSGNIDFATIGGAQAAIRSKARGLDMNIIAST
                     SNYTNYTLIGNKDIKRLEDLRGKIVGITGAGSFSDFTVRLYLKRNNLEPDKDVTLRSI
                     GQTVVRASALEKGLIAAAPFSAEDTVKLLDKGFAMIANFNEALRVPQSVYVSRGEMLE
                     KSPDTTKRFLKALILGMQFAKFNKQEAIKIGFAAGLQGDPEIVQRSYDLFSPGYAADM
                     SVAWDGIQTMLDDDHRTGLVDKKFTLDRVVNDRILKQAQQELKNEGRLRQ"
     gene            4339..>4806
                     /locus_tag="VE170_18345"
     CDS             4339..>4806
                     /locus_tag="VE170_18345"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HYJ17462.1"
                     /translation="MVQELTAGIPYTVDTGEKLVNETFGPNNIRRRKTGSHDLMPMSV
                     RNGRLLAGETSLERNGFILVEHKTKVADFFDSAQLESVYYPEVEQLIKQQSGASRVVI
                     FDHTLRSGDEAERETKLIREPVMSAHNDYTEWSGPNRVREFLPDEAEDLLTRRF"
BASE COUNT         1049 a         1505 c         1328 g          924 t
ORIGIN      
        1 tgaggcggaa aaaggcaagt tggaaatcac cccgatcttc gccgagtcta ttcataaaat
       61 cgtccgcgac ttcttcgcca tgccgccggc gatcaaaaca cggctggaaa aagctctgcg
      121 gaagtaagga gtaatcaaca agccagtcga gcatccaaga ctccttgcta tcaaccatac
      181 tcttcacttg ttcccttaag ccggcagccg ccgccgcgct tcatccgcgt ccaccaccgg
      241 attcttcggc cgtttgcttt cgagcacgcg gatgacttcc tgtgccgcgc cccactcgcc
      301 gcgctcggag gcttccaagg tcaccgcgcc caaatggggc gtccacagaa cattgtcgag
      361 cttgaacagc ggatgatcct tggctggcgg ctcgcgggcg atcacgtcca gcgccgcgcc
      421 ggcgagcttg cgctgctcca gggtccggca cagcgcagct tcgtcgacga tgccgccgcg
      481 cgccatattg atcagaaacg ccgagggttt catcagcgcc aattccttgg cgccgatcat
      541 gtcgcgggtc tcgcggttga gcgggcagtg gatcgtgacg aaatccgcgt ctgagagaag
      601 ctcgctaagc ccaatccact tgccgcccaa agcggtgaca tgggactcag ggatataagg
      661 atcgtagacg agaaaaggca tctcgaaagc ccgagcccga agcgccatgc gcgcgccgat
      721 gttgcccatg ccgacgatgc cgagagattt gccggcgagc tcggtgccga taaacctgag
      781 ataatcgctc cagcgattat cttttaccgc gcggtcggcc gcatgtgtat ggcgcaccag
      841 cgcgatcatc tggccgaagg ccagctcggc gaccgacacg gcgttgctgc ccggctgatt
      901 ggtcacccaa actttgagtt ccttcgccgc ctccaggtcg actcgcgacg gatcgacgcc
      961 gacgccggtg cgggcgacga ttttcaaacg cccgccgccc ttttccatat gttcgcgggt
     1021 gatttggcct ttgcggctga tcaccgcgtc gacccgcgcc gcttcgatat cccagcgctg
     1081 cggctccacc catggattga ccaccaccag gtcaacccgc tcgcgcaacc attgcaaaac
     1141 ctccggttga aactcctcga ccaacacaca tccgagtgac attttcagac ctccgcttcc
     1201 tttgcagcac atctcgcggc attttagatg cgcccgctac ggaattctac ggccaatcat
     1261 ctgtgatggg attgacctct gctagttgct tcatggcgcg atctcgctat actggccaac
     1321 aattttaccc tcgattaaaa aagctatgac ccataaaact attctcagtc ttacactcct
     1381 gttggccttg ctcattcctg cagttaacgc tccggcggag aaagtccgcc tcgccctgcc
     1441 gtcgcgctcc atgggctatc tgccgatgtt catcgcgctg catcgcggtt tcctcaaaga
     1501 cgaaaatctc gaactcgaac tgccggcgct gctgcccaat atcgcccaca acgcgctcct
     1561 gagcggcgag gtggaatacc atggtgtcgc cgactcggcg cttagactag cggccaaagg
     1621 agcgccgctg aagtcgattt ttttcagcgc ccggttgccg aattacttcg tcatgtccag
     1681 gccggcgatc aaatcggtca gcgaactgcg cggcaaacta atcgcggtga gccgttttgg
     1741 cggcaccacc gacctggcgg ccagggtcgc gctccaagcc aacggcctcg aaccgcagaa
     1801 agatgtcgtc ctgatcatga tcggcctggg caacacgcgc aacgccgcgc tcatcgccgg
     1861 ctcggtggac gccaacattg ccaacccgcc ggacaactcc atgctcaagc aaaaaggctt
     1921 tcgcgagttg ctgtttcttg gcgacgctat cgagtttccc agtaacggct ttaccaccac
     1981 cgagcgccgg ctggtggaga accgcgatca agtgaaacgg ctgctgcgcg ccttttaccg
     2041 cggcctgttg ttctcccgtg agaaccctga agagtcaatc aagatcgtcg agcgcgaatg
     2101 gaagctcgat gcggcgacgg cgcgcgactc ctatcaatcg atcatgaagg ccgccagccg
     2161 cgacggcgcc tcatcggagg ccggcctaaa ggtccacatc aaattgatcc agaactccga
     2221 caaaagcatc ggcgatatcg cgttgaccaa gatcgtcgac tttcgcatcc tcgaagaggt
     2281 ccggcgcgaa aacccgcgat gagagcatta ccatgaaact gactatcgtc ttcgtctttg
     2341 cgcttctcgc accgtctgat gtttatgcag agcgtgtgcg cacggtcatt ccgcgcgcta
     2401 cattgaacta tctgagcgtg ccggtggccg agctcaaagg ctactttcgc gatgagaatt
     2461 tggaaaacga aaccatcgtc attcccggca gcagcgccat cgcggccttg gtcagcggcg
     2521 aggtcgatta tagcggcgcc ggcggcaccg gcatgcgcgc ggcgctgcgc ggggcgccga
     2581 tcaaagcggt tatgtttcag accgagaaag taacctggta tttgatcgcc acgccggaga
     2641 tcgccaaggc gggcgatctc agaggcaaac gggtcgccgt cggcacagtg ggcgacaccc
     2701 aggattcgct gattacgctc tacgctgacc gcaacggggt cgcgccgcgc gatctgatcc
     2761 gggtggccat gccgtcgcgc tcgaccacca cgaccatctt gggactgaaa tcgggcgcca
     2821 tccacgctgc gacggtgaac ggcgacgaag cgctcatcgc agagaaggaa gggctacgca
     2881 ccctgacttt tttaggcgac ctcttcccct acccttttca aggtttcatg gcgagcgaca
     2941 agaaaatcgc cgagcggccg ggcgacgtca aacgctggct gcgcgccatg atgcgcgccc
     3001 tcatgttcat ccgcgagcgt cccgaggaag ccgccgacat cgccatgaaa aaaatcccca
     3061 tgggcaaccc aaaccgtccc atcgtcatcg aaggcatccg ccgctttgtc cgcgccttgc
     3121 ccgacggcat tcccggtgtg ccatcggcgc aagggatcaa gaacgtcatc gagttcgacg
     3181 tcaaagcgcc gctcaaaatc aaagacgagg tcgcgccgga aaagtttctg gcgctgaagc
     3241 tcgccggcga agtcagagaa gagttggaac gcaaacgcta gcacagggcg caacgatgta
     3301 ctctcgctgt tgcagaatgg tacccgttct attgctactg tcgctcctat ccgcccagct
     3361 cgcgtggggc caggaaaaga aaaacctccg tgtcgtcttc gtcagcttgg cgtggaatgc
     3421 gcagctgcct ttccgcgtcg ccaatcaccg cggctacttc cgcgatcagg gtcttaccgt
     3481 agagcaaatc ttcgtcagtg gcggcccaac ggcagtcatg gcactggtgt cgggaaacat
     3541 cgacttcgcc accatcggtg gcgcccaggc ggcgatccgc agtaaagcac gcggccttga
     3601 catgaacatc atcgcttcga cgtcgaacta caccaactac acgctcatcg gcaacaaaga
     3661 cattaaacgg ctcgaagacc tgcgcggcaa gattgtcggc atcactggcg ctgggtcctt
     3721 ttctgatttc accgttaggc tctatctcaa acgcaacaat ctcgaacccg acaaagacgt
     3781 caccttgcgc tcaatcggtc aaaccgtggt gcgcgccagc gcgttggaaa aaggactgat
     3841 cgccgccgcg cccttttccg ccgaagacac cgtcaaactg ctcgacaagg gcttcgccat
     3901 gatcgccaat ttcaatgagg ccctgcgcgt gccccagagc gtatacgtca gccgcggcga
     3961 gatgctggag aaaagcccgg atacgaccaa gcggttctta aaggcgctga ttctcggtat
     4021 gcaattcgcc aagttcaaca aacaggaagc gatcaagatc ggcttcgcag ccggcttgca
     4081 gggcgacccg gaaatcgtcc agcgttctta cgatctgttt agccccggtt acgccgccga
     4141 tatgtcggtg gcctgggacg gcattcaaac catgctcgac gacgaccacc gcaccggctt
     4201 ggtcgacaaa aaattcaccc tcgaccgggt cgtcaacgat cggattctca aacaagccca
     4261 gcaggagttg aaaaacgaag ggcgcttgag acaataagtt tgtgctaggg tagaccaagc
     4321 caggaaggag cttcagtcat ggttcaagaa ctgaccgccg gcattcccta caccgtggac
     4381 acgggtgaaa aactcgtcaa cgaaaccttc ggcccgaata acatacgacg gcgcaagacc
     4441 ggcagccacg acctaatgcc gatgtcggtg cgcaacggcc gtttgcttgc cggggagact
     4501 tcactggagc gaaacggctt catcctggtc gagcacaaaa ccaaagtcgc tgatttcttc
     4561 gacagcgcgc aattggaatc ggtctactac ccggaagtcg agcagttgat caaacagcaa
     4621 tcgggcgcgt ctcgggtggt gatcttcgat cataccttgc gttccggcga cgaagccgag
     4681 cgcgagacca aactgatccg tgaaccggtc atgtcggcgc ataacgacta tacggaatgg
     4741 tcgggaccca accgtgtgcg tgagttcctg ccggacgaag ccgaggactt gctgacccgc
     4801 cgcttc
//
LOCUS       DASQKS010000709         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_383065, whole genome shotgun sequence.
ACCESSION   DASQKS010000709 DASQKS010000000
VERSION     DASQKS010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383065"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VE170_18350"
     CDS             complement(<1..1114)
                     /locus_tag="VE170_18350"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HYJ17463.1"
                     /translation="MSEAVSNLRPEPDKLLTEIAGYAASYVSTSREAIDTARYNLLDT
                     LGCGLLALRFSECTKLLGPVVPGAMLANGARVPGTDWQLEPVQAAFNIGAMIRWLDYN
                     DTWLAAEWGHPSDNLGGILALADYLSRARQKSFTVADILQAMIKAHEIQGILALENSY
                     NRVGLDHVLLVRVATSAIATAMLGGTHEQIVSALSNAWTDGGALRVYRHTPNTGPRKS
                     WAAGDATSRGVRLALMARQGEMGYPSVLTAKGWGYYDVLFKGRPFTMGRPLGSYVMEN
                     VLFKISFPAEFHAQTAVECAVQLHEQVRNRLADIDKVTITTQESAIRIIDKSGPLHNP
                     ADRDHCIQYMTAVGLIFGELSADHYEDKIARDPRIDA"
     gene            complement(1216..2334)
                     /gene="prpC"
                     /locus_tag="VE170_18355"
     CDS             complement(1216..2334)
                     /gene="prpC"
                     /locus_tag="VE170_18355"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HYJ17464.1"
                     /translation="MAEKKSGGLEGVIAGSTAISTVGKQGVGLTYRGYSIEDLAAHST
                     FEDVAYLLLYGQLPTADELDEYKARLRNLRALPATLKTVLEQIPASAHPMDVLRTGCS
                     MLGTLEPENAGRTQIAVANRLTACFSGMLLYWHHFVTSGTRIHTETDDDDTAGHFLNL
                     LHGKKPGDLHRRAIDASLILYAEHEYNASTFTARTIAATLPDFYSAVVGGIGALRGPL
                     HGGANEAAMELIEQFSTPDAAEKGLLQMMAAKKLIMGFGHRVYKVSDPRSNVIKAWSK
                     QLGEAKGDSKLYEISERIEEVMRREKKMFPNLDFYSASAYHYCGIPTIMFTPLFVVSR
                     ITGWAAHIIEQRADNRLIRPNADYIGPEPRPFVPVNKR"
     gene            2558..>2907
                     /locus_tag="VE170_18360"
     CDS             2558..>2907
                     /locus_tag="VE170_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYJ17465.1"
                     /translation="MALPQTFKAMVVSETAEKTFVREIKQRALSDLPAGELVIEVKYS
                     SLNYKDALSASGNKGVTRKYPHTPGIDAAGVVVTSTTQMFSAGEQVTVTGFDFGMNTS
                     GGFGQYISVPALWAA"
BASE COUNT          595 a          779 c          904 g          629 t
ORIGIN      
        1 aggcatcgat gcgcgggtcg cgggcgattt tatcttcgta gtgatcggcg ctaagttcac
       61 cgaagatcaa gccgacggcg gtcatatatt gaatgcaatg atcgcggtcg gcgggattat
      121 gcagcgggcc ggacttgtcg atgatgcgga tcgccgactc ctgggttgta atggtgactt
      181 tgtcgatgtc ggccaatcta ttcctgacct gctcatgcag ttgcacggcg cactcgacag
      241 cggtctgggc gtgaaattcg gcgggaaaag aaatcttgaa caaaacattt tccatcacat
      301 aggagcccaa tggccggccc atggtgaacg gcctgccttt gaacaggaca tcgtagtagc
      361 cccaaccctt ggccgtgagt accgacggat aacccatctc gccttgccgc gccatcagcg
      421 ccaagcgcac gccgcggctg gtggcgtcgc cggcggccca ggattttctt ggcccggtgt
      481 tgggcgtatg gcggtagacg cgcagggcgc cgccgtcggt ccaggcgttg gatagcgcgc
      541 tgacgatttg ttcgtgcgtg cccccgagca tcgccgtggc gatagcgctg gtggcgacgc
      601 ggaccagcag gacgtgatcc agaccgacgc ggttgtaact attctccaac gccaggatgc
      661 cttgaatctc atgggccttg atcatcgcct gcaagatgtc ggcgactgtg aacgatttct
      721 gtcgggcgcg actgaggtag tcggcaaggg cgaggatgcc gcccaagttg tccgacgggt
      781 gaccccactc ggcggcgagc caggtatcgt tgtagtcgag ccagcgaatc atcgcgccga
      841 tattaaacgc cgcctgtacg ggctccaact gccaatcggt gcccggcact cgggcaccgt
      901 tggccagcat cgcgccggga accaccggac cgagaagctt ggtgcattcg gaaaaacgca
      961 gggctagcaa gccgcagccc agggtgtcga gcaaattata gcgcgctgtg tcgatggctt
     1021 cgcggcttgt cgagacgtaa cttgccgcat acccggcaat ctcggttaat aatttgtctg
     1081 gttcaggtcg caggtttgag acagcttcgc tcatggtttc actcaatact gttcaaaggc
     1141 ccaagcgttc aaggttcaag ggtcaatcgg accggctttc atggaacttt agaactgttg
     1201 aaccttgaac ggaagttatc ttttattgac cggcacaaag ggccgcggct cggggccgat
     1261 gtagtcggcg ttggggcgga tcaagcgatt gtcagcgcgc tgctcgatga tatgcgctgc
     1321 ccagccggtg atgcgcgaga ccacgaacag cggcgtgaac atgatcgtcg gaatgccgca
     1381 gtagtgataa gcgctggcgc tgtagaaatc caagttggga aacattttct tttcgcgccg
     1441 catcacctct tcgatgcgct cggagatttc ataaagtttg ctgtcgccct tggcctcgcc
     1501 gagttgcttc gaccaggcct tgatgacgtt ggaacgcgga tcggaaactt tatagacgcg
     1561 gtggccgaag cccatgatga gttttttcgc cgccatcatt tgcagcaaac ctttttcggc
     1621 cgcgtccggg gtcgaaaact gctcgatcaa ctccatggcg gcttcgttgg cgccaccgtg
     1681 cagcgggcca cgcaacgcgc cgatgccgcc gacgacggcg gaataaaagt ccggcagcgt
     1741 ggcagcgatg gtccgggcgg taaaagtgga agcgttgtac tcatgctcgg cgtagagaat
     1801 cagcgaagca tcgatggcgc ggcgatgcag atcgccgggc ttcttgccgt gcagcagatt
     1861 gagaaaatgg ccggcggtat cgtcgtcgtc ggtttcggtg tgaatgcgcg tgccgctggt
     1921 gacgaagtga tgccagtaga gcagcatgcc gctgaagcag gcggttagcc ggttggccac
     1981 cgcgatctgg gtgcggccgg cattttccgg ctccagggtg cccagcatcg aacagccggt
     2041 gcgcaggaca tccatcggat gggcgctggc tgggatttgt tccaaaacgg ttttcaaggt
     2101 cgccggcagg gcgcgcagat tgcgcaggcg cgctttatat tcgtccagtt cgtccgccgt
     2161 gggcagctgg ccatagagca ataagtaagc gacgtcttcg aatgtcgaat gggcggccaa
     2221 gtcttcaatg gaataacccc gataggttag accgacgccc tgtttgccga ctgtcgagat
     2281 cgcggtgctg ccggcaataa cgccttcgag accgccagat tttttctctg ccattgttca
     2341 atcctccaat tcgacaatgc tacgaattcg gtaatcaaat caagcggggg cgtcgctcct
     2401 gtccggcgtt aaaaaccaaa ttttcgttca agggttcaag agttcaaggt tcaaggtcgg
     2461 aatggggaat gagctgcttt cattgaccct cttgacagtt cgccggtgga ttggataccg
     2521 ctggtgtatt cattttttcc aaggagcaat ggactcgatg gctttaccgc aaacattcaa
     2581 ggcgatggtg gtttccgaaa cggctgagaa aacatttgtc cgcgagatta aacaaagagc
     2641 gctcagcgat ctgccggccg gcgagctggt tatcgaagtg aaatactcgt cgctcaatta
     2701 caaagacgcg ctatcggcga gcggcaacaa aggcgtgacg cgtaaatatc cgcacactcc
     2761 gggcatcgat gcggcgggtg tcgtcgttac ttcgacgacg caaatgttct cggccggtga
     2821 gcaggtgacg gtgacgggat tcgatttcgg catgaatact tcgggcggct tcggccagta
     2881 catcagcgtg ccggcgctgt gggcggc
//
LOCUS       DASQKS010000710         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_839493, whole genome shotgun sequence.
ACCESSION   DASQKS010000710 DASQKS010000000
VERSION     DASQKS010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839493"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VE170_18365"
     CDS             complement(<1..196)
                     /locus_tag="VE170_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17466.1"
                     /translation="MWRYFKKRLDGWKTIIWNGFLGVAPVVLVSLDKLASLDLSQYMT
                     WWLAILVGFVVSAVGVWLRYI"
     gene            complement(199..348)
                     /locus_tag="VE170_18370"
     CDS             complement(199..348)
                     /locus_tag="VE170_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17467.1"
                     /translation="MWSIFDIIVFVGGFAACWFAKDRLIKSYDGAANFVRRLGAKAKA
                     IRESL"
     gene            complement(354..671)
                     /locus_tag="VE170_18375"
     CDS             complement(354..671)
                     /locus_tag="VE170_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17468.1"
                     /translation="MIYRKSTIRLLAAAAFLIIARLVFDLTSATAYAYPEFDFGQNGI
                     AFVVFILAVPAVLVLALVRLFQRRFPAVILSDPKPIDVPPLTSRPATGWLAAILNVIF
                     KRR"
     gene            complement(953..1303)
                     /locus_tag="VE170_18380"
     CDS             complement(953..1303)
                     /locus_tag="VE170_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HYJ17469.1"
                     /translation="MKKLLVGALIASSFLPFEARAQERAGSAALGAVSGAVVLGPIGA
                     VAGAFIGYTAGPSIARSWGMQRPSSRSRARHATQSGRAPQQQAAVATSSPPPVRTSPP
                     SVKRAALPPVQGFE"
     gene            complement(1514..2437)
                     /locus_tag="VE170_18385"
     CDS             complement(1514..2437)
                     /locus_tag="VE170_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17470.1"
                     /translation="MVTAARARPVCRVAFAGLLVCLSGLASGRELDSDDDESATAAST
                     PNLYLDLRTNYATFPAGSLAIGFGGFGFLAGRPGLSALAGLSNLPTLPTVSSPSGQSV
                     GLDVPLTIDLNDRVSVYGGFSTSASRSDLSDWSALTIYSWSVGVQAEVYQQNGGSIPT
                     ITVQSTLSLPVPHAPLPTTSLNTILEFDYALNVDETRGLLAGIQHTGATVNSGFLSIK
                     PSIVGYLGGYYQWDNNWKFTGRVGVQSFGGLQFLNQTPFEPFTQPIVRLDLDRMDDDD
                     NRLFGVTAVIAWVPRPSYQLTVRTPLYLVKN"
     gene            2738..2956
                     /locus_tag="VE170_18390"
     CDS             2738..2956
                     /locus_tag="VE170_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17471.1"
                     /translation="MAIPDRDDVPGGKTRTRLAAMAASSIRSKIAAALKARPTPVSED
                     SDNVASHAFVALLKQFFAKDPRFSVRAV"
     gene            3047..3232
                     /locus_tag="VE170_18395"
     CDS             3047..3232
                     /locus_tag="VE170_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17472.1"
                     /translation="MYFVAAVLAIMSALFYAAGNQEIGTLGVEMCRYGSTFCRSPTYV
                     LAGAILAGLWGFFVSVR"
     gene            3478..>3785
                     /locus_tag="VE170_18400"
     CDS             3478..>3785
                     /locus_tag="VE170_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17473.1"
                     /translation="MKWVVERDLLIAQTMAFVQSVTGKKPEAEAQLESPSSPVVQIEQ
                     PESPAEVVEKPRRPLVARSPVREEIEGRVAAFRAHQQRFDRERDRHFNSVLTGIRAAI
                     T"
BASE COUNT          815 a         1114 c         1114 g          742 t
ORIGIN      
        1 tgatgtaacg tagccaaacc ccgacggctg acacgacgaa gccgacgagg atggccaacc
       61 accaagtcat gtattgcgac aggtcgagtg acgcgagctt gtcaagcgag accagcacga
      121 ccggagcgac gccaagaaat ccgttccaga tgatcgtctt ccagccatcc agcctcttct
      181 tgaaataccg ccacatggct atagcgattc cctgatcgcc ttggccttgg ctccaagtcg
      241 cctgacgaag ttggcagccc cgtcatagga tttgatgagc cggtccttgg cgaaccagca
      301 ggccgcgaag ccgccaacga atacgatgat gtcaaagatc gaccacatga ggattatctc
      361 cgtttaaaaa tgacgtttag gattgcagcc agccaacccg tcgcgggcct ggaggttaac
      421 ggcggaacat cgatgggttt gggatcggag agaatcactg cgggaaaccg cctttgaaaa
      481 agacggacca aagccaatac caggacagcc ggtacggcga ggatgaatac aacaaacgca
      541 atgccgttct ggccgaaatc gaattccgga taggcataag ccgtagccga ggtgaggtcg
      601 aacactagcc ttgctattat gaggaacgcg gcagccgcaa gaagcctgat tgtcgatttt
      661 cgataaatca cactgccatt ccttcaaacg ccccctgctg cgtttgatct tctatgcttg
      721 gggacccttc gcgtgtttcg tccctaccta tcgccgattc cttcaggaga ttttgcgcag
      781 gagaagtggc ggagcaaagg acaagcggcc ccgacacagc agaccggatt taaaaaaccc
      841 gcgaccggtt tcccggcgcg ggcttttgaa ttcttgcgat gatgaagata tgccggtgat
      901 ttgcccgacg tgtcaaatgt cctgtcgcga ccgcggattc atccccgcgc ttttattcaa
      961 atccctgaac cggtggcagc gcagctctct tgacggaagg gggcgatgtc ctgaccggtg
     1021 gcggcgaact tgtcgcgacg gccgcctgtt gctgcggcgc acggccggat tgtgttgcat
     1081 gccttgcgcg cgatcgcgat gagggtcgct gcattcccca ggagcgcgcg atcgacggtc
     1141 ctgcggtata tccaatgaac gcaccagcaa cggcgccaat cggtcccagc acgacggcgc
     1201 ccgacaccgc gcccagggcc gcgcttccgg cacgctcctg tgcccttgct tcaaacggga
     1261 gaaacgaact tgcgatcagt gcgccgacaa gcagtttctt catcgccatt tccttgagcc
     1321 cagcaacgtc accggactgc gaaaatgcgg cggcacaaag gagatgtggc ggcaacaatc
     1381 gctcgattca aatctcccga tgcaaaaagc ccgcggccgg ttccggcgcg ggcttttgaa
     1441 ttcttgcgat gctgcattta tgccggtgat ttgcccgacg tgtcaaaact ttttctgacg
     1501 cgcgcaaaac gcgctagttt ttcaccaggt aaagcggcgt tcgcaccgtc aactggtagg
     1561 atggcctcgg cacccacgcg atcaccgccg tcacgccgaa caggcggttg tcgtcgtcat
     1621 ccatccggtc gaggtcaagc ctgacgatgg gctgggtgaa aggctcgaac ggtgtctggt
     1681 tgaggaactg cagaccgccg aacgattgca cgccgacgcg gccggtgaac ttccaattgt
     1741 tgtcccactg atagtagccg ccgaggtagc cgacgatact tggtttgatg ctgagaaatc
     1801 cgctattcac cgtagccccg gtgtgctgga tgccggccag cagcccccgg gtctcgtcca
     1861 cgttcagggc gtagtcgaac tcgagaatgg tattgagcga tgtcgtcggc agcggcgcgt
     1921 gcggcaccgg cagcgaaaga gtcgactgca ccgtgatggt cgggatcgat ccgccgttct
     1981 gctgatagac ctcggcctgg actcctacgc tccagctata gatcgtgagc gccgaccagt
     2041 cagacaggtc gctgcgcgat gcgctggtgc tgaagccgcc atacaccgaa acccggtcgt
     2101 tgagatcgat cgtgagagga acatcaagcc cgacgctttg gcccgacggc gacgacacag
     2161 tcggtagcgt cggaaggttc gagaggcccg cgagggccga cagccccggg cgcccggcga
     2221 gaaatccgaa accgccaaaa ccgatcgcaa gcgagccggc tggaaaggtg gcgtagttgg
     2281 tcctcaggtc gagatagagg ttgggcgtcg aagcggctgt tgccgattca tcgtcgtcgc
     2341 tatcgagctc tctgcccgac gcgaggccgg acaagcaaac caaaagcccc gcgaacgcca
     2401 cgcgacagac cggccgggcg cgcgccgccg ttaccattcc aatgtcctcc agtaacccgc
     2461 agttataccg ccgcactgac tgcgttcaag agatacaatc gccttggaac gtctttgctg
     2521 cccgattgaa gaggccggca accgcaggag gacgtgacga gacggcgcca gctctctggt
     2581 caggcttggg cacaggcagc aagcagacca cggcagagtc cgtaatcttc cggattgggc
     2641 aaccgaagta tgccgcaact ctcgcggtgg attcttttgg agtttatcga ggtatccaat
     2701 acagcgtcgc gcttaccgtc gagccggacg tctggcaatg gcaattccag atcgcgatga
     2761 cgtcccgggc ggcaagacca gaaccaggct tgccgccatg gccgccagca gcattcggtc
     2821 gaaaattgct gcggcgctca aggctcgccc gacacccgtt agcgaggaca gcgataacgt
     2881 cgcatcacat gcatttgtcg ccttgttgaa gcaatttttc gcgaaagatc cacgtttcag
     2941 tgtcagggct gtttaagtcc tgggtcacag aataggcgac tcttgtgcgg aacgattccg
     3001 cagacttttc cagggaaaca agctccgagg aattccgagt aagcccatgt attttgtagc
     3061 cgccgtactg gcgatcatgt ccgccctgtt ctacgccgcc ggcaatcagg aaattggcac
     3121 gctcggcgtg gagatgtgcc gatacggcag cacattctgt cgcagtccaa cttacgttct
     3181 ggccggcgcc attctcgcgg ggctgtgggg cttttttgtc agcgtccgct gagcaaccga
     3241 gcgaagccat cgcgaccgcg gcagccggga aagcggctgt cgacgatcta cgctattaac
     3301 ccgcaagcgc cgcttgggag cctgtttgtt gcattctgcg cggcgtcgcg gtggcccgat
     3361 ggcactggat tcgcacattt cgaccgtcaa ttgcttcccc acgcaggcct cacaggcaaa
     3421 aaccagcttg attcatcggc cgctttttgc tgaaatcacc cttgggtgag gtgcgtgatg
     3481 aagtgggtag tggagcgcga tcttttaatc gctcaaacaa tggcgtttgt gcagtctgtc
     3541 accggcaaga agccggaggc tgaagcgcag ctcgaatcgc cctcatcccc cgtggtccaa
     3601 attgaacagc ccgaatcgcc tgctgaagtc gtggaaaaac cgcgcaggcc actcgttgcc
     3661 cggagccccg ttcgcgaaga gattgaaggc agggtggcgg cttttcgagc ccatcaacag
     3721 cgcttcgatc gggaacgcga caggcatttc aattccgtat tgacgggaat tcgcgccgcg
     3781 atcac
//
LOCUS       DASQKS010000711         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_390891, whole genome shotgun sequence.
ACCESSION   DASQKS010000711 DASQKS010000000
VERSION     DASQKS010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390891"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VE170_18405"
     CDS             <1..387
                     /locus_tag="VE170_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17474.1"
                     /translation="MDIQYLQNVLVALRGYVKTHRDLASRFMKGFVEGVAHFKRNKED
                     SLKILMKKMRIEKGKETYLERSYGLYATQYIESAPYPSVAGTRTVLEFMAKDFPKAKT
                     ADPNSFIDNSLIKPLEDSGFIKGLYR"
     gene            complement(426..1535)
                     /locus_tag="VE170_18410"
     CDS             complement(426..1535)
                     /locus_tag="VE170_18410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17475.1"
                     /translation="MYIDGDTHYWPLRFIDKVKHPGKGHLEVVEDKGDMVRYGEVVPG
                     KVATYYRDGKKVHSFKEGRWSIALRAEFMKKDGFDKQVLIPDNRPLIYECDAELGRQL
                     ARAYNDTVAEDIQGDDRFIGCAWIYLPDIKESVKELRRAVNELGLKAIKLNGGWGDGD
                     LDSEVLYPLYEEIANLNIPILLHPAARVYEAQHSHPWLVGSERFQGYQHFPTALGFPL
                     TYMVSASRLIFGGVLDRFPSLKFGFFEGGIGWVPWLMHTLDAHMPNDATKSVAVQQFF
                     EGKQGIKKQPSQYFEQFFIACVSWETYIADTVNGWPNHNIIIGSDFDHGDAVATWPRT
                     VEVIKQMKSLSATDKEKVLGGNAMRMFGLNGATAH"
     gene            complement(1603..1779)
                     /locus_tag="VE170_18415"
     CDS             complement(1603..1779)
                     /locus_tag="VE170_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17476.1"
                     /translation="MKGFTEKYNVNKLVYYEQTNDVFTALTREKEIKKWRREKKNALV
                     VQLNPHWNDLSSAI"
     gene            complement(1991..>2788)
                     /locus_tag="VE170_18420"
     CDS             complement(1991..>2788)
                     /locus_tag="VE170_18420"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17477.1"
                     /translation="AAMASNDIEVGYTGGTAVIGAVASGADLKVLSALTNRVTYELVA
                     RPGIKTPEDLRGKKFGVQAIGGTVWMGAILALEHLGLDPDRDKIAIIAAGDQTVLAQA
                     VANGTIDATVLDGVQSRPLQAKGFPILAELEKAKLPILSSSIVVREPYLQKNPQIVEN
                     VLRAVIEGAAFCLSAAQKPSTLKILQKYLRISEKDAEEGYRDMVMGLDRKPYANPAGA
                     TNVIRLMKRSNPKIESVKPENLIDDRIMKKLDQSGFIDEIMTKYGVK"
BASE COUNT          594 a          789 c          724 g          681 t
ORIGIN      
        1 atggacattc aatatttgca aaacgtcttg gttgctttgc gaggttatgt aaaaacccac
       61 cgcgatttgg cgagccgctt tatgaaaggt ttcgtcgaag gcgtcgccca cttcaaacgc
      121 aacaaagaag acagtctcaa aatcctcatg aagaagatgc gcatcgaaaa aggcaaagag
      181 acctatttgg agcgctccta cggactctat gccacgcaat atatcgagtc ggccccctac
      241 ccctccgtgg ccggcaccag gaccgtgctc gaattcatgg ccaaagactt ccccaaagcc
      301 aagaccgccg accccaactc ttttatcgac aatagtttaa tcaagccgct ggaagacagc
      361 ggatttatca aggggctgta tcgataaata tttagcgcaa gttcgtgctg aaaatcgaca
      421 gctcgtcagt gggctgtcgc gccattcagt ccaaacatcc gcatcgcgtt gccgcccaaa
      481 accttttcct tgtcggttgc cgacaaagac ttcatctgct tgatcacctc caccgtgcgc
      541 ggccaggtgg cgacggcgtc gccgtggtcg aaatcgctgc cgataataat gttgtgattg
      601 ggccaaccgt ttacggtgtc ggcaatgtag gtttcccagg aaacgcaggc gataaaaaac
      661 tgctcgaagt actggctggg ctgtttcttg atgccctgtt tgccttcgaa gaactgctgt
      721 accgcgaccg atttcgtggc gtcattgggc atatgggcat ccagcgtgtg catgagccat
      781 ggcacccagc cgatgccgcc ctcgaagaag ccgaacttca aactcggaaa ccgatcgagc
      841 acgccgccga agatcaatcg cgacgcgctg accatgtaag tgagcggaaa gcccagcgcc
      901 gtgggaaaat gctgatagcc ctgaaagcgc tcgctgccga ccagccacgg gtggctgtgt
      961 tgagcttcat acacacgcgc cgctggatgg agcaggatcg gaatattcag gttggcgatt
     1021 tcttcgtaaa gcggatagag cacttcactg tccaaatcgc catcgcccca gccgccattg
     1081 agcttaatcg ctttcagacc aagctcgttc accgcgcggc gcagttcttt tacggattcc
     1141 ttgatgtccg gcaaataaat ccaggcgcag ccgatgaaac gatcgtcgcc ctgaatgtcc
     1201 tcggccaccg tgtcgttgta ggcccgtgcc agctgccggc cgagttcggc atcgcattca
     1261 tagatcagcg gccggttatc ggggatcaaa acctgcttat cgaagccgtc tttcttcatg
     1321 aactccgcgc gcagtgcaat gctccagcga ccttctttga acgagtgaac tttcttgcca
     1381 tcgcgatagt aggtcgcgac cttgcccggc acaacctcgc cgtagcggac catgtcgcct
     1441 ttgtcctcga ccacttcgag gtggcctttg ccgggatgct tcaccttgtc gatgaaacgc
     1501 agcggccagt agtgggtatc gccgtcaatg tacatgactc aatctccctc acacatgcta
     1561 ttgcagccac ccctgcctgt catttcgacc gcagggagaa atctagatcg cactactcag
     1621 atcattccaa tgtggattga gctgtacgac caaggcgttt ttcttttctc ggcgccattt
     1681 tttgatctct ttttcccgag tcaaagcggt gaatacatcg ttagtctgtt cgtagtaaac
     1741 cagcttgttg acgttgtatt tctcggtgaa acctttcact aacttgttct ggtgtttgta
     1801 aacacgtctt gtgagattat tggttaccaa cgtacatgac tttattgttc gaactagtca
     1861 gcaaatagac gtagtagcag ttctctgccg gcatagattt ctcgctacgc tcgaaatgac
     1921 aaaacgtaag attctacttt tatgtcaaga tccctcactt tgttcgggat gacgactact
     1981 accgccgccg tcatttcact ccatacttgg tcatgatctc atcgatgaac ccgctctggt
     2041 cgagcttctt catgatccgg tcgtcgatga gattttccgg tttcacgctc tcgatttttg
     2101 gattgctccg tttcatcagc cggatgacgt tggtcgcgcc agccgggttg gcgtagggtt
     2161 tgcggtcgag acccatgacc atatcgcgat agccctcctc ggcgtctttt tccgatatgc
     2221 gcagatactt ttgcagaatc ttcagtgtgc tgggtttttg cgccgcggac aggcaaaagg
     2281 ccgcgccttc gattaccgcg cgcaatacat tctcgacgat ctgcgggttt ttctgaaggt
     2341 acggttcacg aacgacgatg gaagaactca aaatcggcag tttggctttt tcgagctcgg
     2401 ccaagatcgg aaagcccttg gcctgcaacg gccggctctg cacgccgtcg agcacggtgg
     2461 cgtcgatggt gccgttggcc accgcttggg cgagcaccgt ctgatcgccg gcggcgatga
     2521 tggcaatctt gtcgcggtcc ggatcgagac caaggtgttc gagcgctaat atcgcgccca
     2581 tccacaccgt gccgccgatg gcttgcacgc cgaatttctt gccgcgcagg tcctccgggg
     2641 tcttgatgcc cggccgcgct accagttcgt aggtgacccg gttggtcagc gccgataaaa
     2701 cctttaaatc ggctccggat gctaccgcgc cgatgaccgc ggtgccgccg gtgtacccga
     2761 cttcgatatc gttggaggcc atggcggc
//
LOCUS       DASQKS010000712         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_391588, whole genome shotgun sequence.
ACCESSION   DASQKS010000712 DASQKS010000000
VERSION     DASQKS010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391588"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VE170_18425"
     CDS             complement(<1..209)
                     /locus_tag="VE170_18425"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYJ17478.1"
                     /translation="MSSIDATGPVIFCHPSDDGAAGVVQLLLDALPLGALLTDGSGKI
                     IMLNQQAERFLGWPAAKLIGQPAHE"
     gene            complement(206..1495)
                     /locus_tag="VE170_18430"
     CDS             complement(206..1495)
                     /locus_tag="VE170_18430"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HYJ17479.1"
                     /translation="MDHEDTITKKRLAIGDVRPGMYIVGLDRSWLQTPFLFHRKLIQS
                     SEEIDQLKRHGIREVVIDPARGVDIAEGRDDTTHRARHAAGLATEDPLAATQAAAVQP
                     PLSAAELAFQPLARELDNARAVREETLTLAQTIFDGVGAGARIDGKIAAQVVTQLQAS
                     IARSPEANLLLMQMRRFQKDLFTHAVNVCVLALVVATAENLGDDIATLGMAALLHDVG
                     ETRLPRALLRKSNNFTDDEAGLLKQHPALGAALLAQTKGIDPSAQSIVLAHHERIDGS
                     GYPSGATDLSLYSQIVALTDSYDDMLSGRNQSALQPTEVLRQLYLQANTGTVERDLVE
                     KVIRSLGVYPIGSVVELNTGEHGVVVATNRAAALKPVVRVIMSRSGLVQFNGPIIGLA
                     DAAPKDAIERRIVGVLDPVKERVDPLVFMRLAPGVLA"
     gene            complement(1772..3205)
                     /gene="purF"
                     /locus_tag="VE170_18435"
     CDS             complement(1772..3205)
                     /gene="purF"
                     /locus_tag="VE170_18435"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYJ17480.1"
                     /translation="MSDKFDKFHDECAVMGIYGHPEAANMVYLGLYALQHRGQESCGI
                     VTSDGKGLISHRQMGLVADAFKEDVIKRLEGQNAIGHNRYSTQGQSHLKNAQPFVVEY
                     SQGPIAIAHNGNLVNGTLLRNELEQAGSIFQSTSDTEVIIHLIATSKEPTLMGRIVEA
                     LSRCRGAYSLQFLTLDKLIAARDPYGFRPLVLGRFPDGKNRGAYVFASETCALDLIEA
                     EYVRDVEPGEILTVGPDGLESLKPFPPVPHAKCIFEYIYFARPDSKLFGHNVYQVRKS
                     LGRQLARESGIDADLVTPVPDSGVPAAMGFAEESKIPLEFGLIRNHYVGRTFIEPQQT
                     IRNFGVKIKLNAQRDVLQGKRVIVVDDSIVRGTTSRKIIRMLRDAGAREVHLRISSPA
                     TTGPCYYGIDTPTRGELIASANSVEEIRRFVEADTLAYLSNDGMYTYFDGDKKGFCDA
                     CFTGNYPVHFEDEGHIKQLHLFDALNR"
     gene            complement(3269..>3680)
                     /locus_tag="VE170_18440"
     CDS             complement(3269..>3680)
                     /locus_tag="VE170_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HYJ17481.1"
                     /translation="PPWIDLKLELAVQSCCIGAIEQGLLESAHDVSNGGLAVALAECC
                     IGGPDKPLGVRVETHEMIRTDALLFSESQSRIIVSLKETNLERLQELAEQHRVPLLVI
                     GAVGGSRLAFPPLLQPPVEELRGIWSNGLTARLK"
BASE COUNT          782 a         1060 c         1083 g          755 t
ORIGIN      
        1 cattcatggg ccggctgccc gatcaacttg gccgccggcc aaccaagaaa ccgctcggct
       61 tgctggttga gcatgatgat tttgccggag ccatcggtga gcagcgcgcc caacggcaaa
      121 gcatcgagca acagttgcac aacaccggcg gcgccgtcgt cactgggatg gcaaaaaatc
      181 accgggccgg tagcatcgat gctgctcacg ccaaaactcc cggcgccaat cgcataaaga
      241 ccaagggatc gacgcgttct ttcaccgggt cgagcacgcc gacgattcgc cgctcgatcg
      301 catccttagg ggcagcatcc gctagtccaa tgatcgggcc attgaactgc accaatccgc
      361 tgcgtgacat gatcactcgc accaccggct tcaacgccgc cgcacggttg gtcgccacga
      421 ctacgccgtg ttcgccggtg tttagctcca ccacgctacc gatcgggtag acacctaagc
      481 tgcgaattac tttttcgacc agatcgcgct ccactgtgcc ggtattggcc tgcagataga
      541 gttggcgcag aacttcggtg ggttgaagcg cactttgatt gcgccccgac aacatatcgt
      601 cgtaactgtc ggtcagcgct acgatctgac tgtagaggct taggtcggtt gcgccggagg
      661 gatagcccga gccatcgatg cgctcatgat gggcgagaac gatgctctgg gccgacggat
      721 cgataccctt agtctgcgct agtagcgccg cgccaagagc cggatgctgc ttcagtaaac
      781 cggcttcatc gtcggtaaaa ttattggatt tgcgcagcag cgccctcggc aaacgagtct
      841 cgccaacatc gtggagcagc gccgccattc ccagcgtggc gatatcgtcg ccgagatttt
      901 ccgccgtcgc caccaccagc gccaacacgc aaacattgac cgcatgggta aatagatcct
      961 tttgaaaccg gcgcatctgc ataagcagca gattcgcttc cggggagcgg gcgatgctcg
     1021 cttgcagctg ggttaccacc tgtgcggcaa ttttgccgtc gatacgcgcc ccggcaccga
     1081 caccgtcaaa gatcgtctgc gccaaggtca acgtctcttc gcgcaccgcg cgcgcgttat
     1141 caagttcccg cgccagcggt tgaaatgcca actcagccgc ggatagtggc ggttgcaccg
     1201 cggccgcttg ggtagcggcg agaggatcct cagttgcaag acccgcagcg tggcgcgccc
     1261 tatgggtagt gtcgtcgcga ccctccgcaa tatccactcc gcgcgcggga tcgatcacca
     1321 cttcacggat gccatgcctc ttgagctggt cgatttcttc actgctttga atcagcttgc
     1381 gatgaaacaa aaacggcgtc tgtaaccaag accggtccaa accgacgatg tacatgccgg
     1441 gacggacatc gccgatcgct agccgttttt ttgtgatcgt gtcttcgtgg tccatgttgt
     1501 cctgcctatc gagagtcaac caggagctgc tggcaagcca cagcgtgccg ccaacagcta
     1561 agctatcggc agccgcacaa agaagttaaa tggcgcggcg cacatggact gagttttcac
     1621 accaggcaac cccatacgca taaaaatgga tactggcgct ctaagggaaa cttagaaata
     1681 gccgatccgg tttccactgt tagaaattga tgagcgtggc acgggacagt gcgcaaggtg
     1741 cgcatgacgc aggagaaagg cagtttagcg cttagcggtt tagcgcgtcg aaaagatgga
     1801 gctgtttgat atggccttcg tcttcgaaat gaaccggata gttgccggtg aaacaggcgt
     1861 cgcagaaacc ttttttgtcg ccgtcaaagt aggtgtacat gccgtcgttg gaaagatagg
     1921 cgagggtgtc ggcttcgacg aagcggcgga tttcctccac cgagttggcc gacgcgatca
     1981 gttcgccgcg ggtcggcgta tcgatgccat aataacaggg accggtagtt gccggcgagc
     2041 taatgcgcag atggacttcg cgggcgccgg cgtcgcgcag catacggatg atcttgcggc
     2101 tggtggtgcc gcgcactatg gaatcgtcta cgacgataac ccgcttgccc tgtaagacat
     2161 cgcgctgggc gttgagtttg atcttgacgc cgaagttacg gatcgtttgc tgcggttcga
     2221 taaaggtgcg gccaacatag tgattgcgaa tcaaaccgaa ctcgagcggg attttcgact
     2281 cttcggcgaa acccatagcc gccggcacgc cggaatcggg taccggcgtg accaggtcgg
     2341 cgtcgatgcc gctctcccgc gccagctggc ggcccagtga cttgcgcacc tggtaaacgt
     2401 tgtggccgaa aagcttgctg tcgggacggg caaagtaaat atattcgaaa atacacttgg
     2461 catggggcac cggcgggaac ggtttgaggg attccaaacc atcggggccg acggtgagga
     2521 tctcgccagg ctcgacatcg cgaacatact ctgcttcgat cagatccaac gcgcaagttt
     2581 ccgatgcgaa tacgtaggcg ccgcgatttt tcccgtcggg aaagcggccc aataccagag
     2641 ggcgaaaacc gtagggatcg cgcgccgcga tcagcttgtc cagagtcaaa aattgcagcg
     2701 agtaggcgcc gcggcagcgg ctcagcgctt cgacgatccg ccccatcaag gtcggctctt
     2761 tcgaagtggc gatcagatga atgatcactt cggtgtccga ggttgactgg aagatcgacc
     2821 cggcctgctc gagctcattg cgcaatagcg tgccgttgac gagattgccg ttatgggcga
     2881 tcgcgatggg cccctgggag tactcgacaa cgaagggttg ggcgtttttg agatggctct
     2941 gaccttgggt ggagtagcgg ttgtggccga tggcgttttg cccttcgagc cgcttgatca
     3001 cgtcttcctt gaaagcgtcg gcgaccagtc ccatctgacg atgggagatc agccccttgc
     3061 catcggaggt tacgatgccg caagattcct ggccgcggtg ctgcagcgcg tagaggccca
     3121 agtaaaccat gttggccgcc tcgggatgtc catagatgcc catcaccgcg cactcgtcat
     3181 ggaacttgtc gaatttgtcg gacatcgtca atttttgcga tcccgcgcca tccgattaaa
     3241 gatttaacga tcgaaatttt aaacgaagtt atttgagccg ggctgtaagt ccattagacc
     3301 agatcccacg caattcttct actgggggtt gcaaaagcgg tggaaacgcc agccggctac
     3361 cgccgacggc gccgatcacc agcagcggca cacggtgttg ctcggcgagc tcttgcagcc
     3421 gttcaagatt ggtttctttg agggagacga taatgcgcga ctgcgactcg ctgaacaaca
     3481 gcgcatcggt gcgaatcatt tcatgggtct cgacgcgcac ccccaggggt ttgtccggac
     3541 cgccgatgca gcactccgcg agtgccaccg ccagaccgcc gttggagacg tcatgggccg
     3601 actccaagag tccctgttcg atggcgccga tacagcagct ctgcaccgcc aattccaatt
     3661 tgagatcaat ccatggcggt
//
LOCUS       DASQKS010000713         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5865321, whole genome shotgun sequence.
ACCESSION   DASQKS010000713 DASQKS010000000
VERSION     DASQKS010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5865321"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /gene="leuS"
                     /locus_tag="VE170_18445"
     CDS             <1..1610
                     /gene="leuS"
                     /locus_tag="VE170_18445"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYJ17482.1"
                     /translation="LMEYGTGAVMAVPAHDQRDFEFAKKYSLPIRMVIQAQDKNLTAD
                     GLTEAYVDEGVMRDSGEFSGTANIAGKEMIAAYAEAHGFGKPAIRFRLRDWGVSRQRY
                     WGTPIPIIYCAKCGVVPVPEDRLPVELPKEVPFSGQGGSPLQESAAFLRVDCPQCGGD
                     ARRETDTMDTFVDSSWYYLRYTSTDSTTAPVDVSRQRYWMAVDQYIGGVEHAVLHLLY
                     ARFFTKALRDIGLTHVDEPFANLLTQGMVSKETYKCAEHGWLFPNELLRTEDGWRCPR
                     CRRAVDKGRVEKMSKSKKNIIDPEDLIAAYGADTARLFTLFAAPPEKDLEWSDQGVEG
                     AYRFLGRLWRLVYQQGDLWSAADGGAAGEKPNAELRDLRRQIHRTIKKVSDDIDGRFH
                     FNTAIAAIMELFNALSAVSQDPAKRAAGAAVLREGLESIMLLLAPFVPHVASELWEQS
                     GHRERLDQVPWPTYSEAALEEEQLLIVVQVNGKVRGKMTVPAGITQERIEASAMADAR
                     VMSFCDGKKIKRVVYVPRRLVNIVVEG"
     gene            1613..2236
                     /locus_tag="VE170_18450"
     CDS             1613..2236
                     /locus_tag="VE170_18450"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HYJ17483.1"
                     /translation="MLRMVNWSVGKAGSWRLVLAGAALILINACGYQWVGQSSLLPKD
                     AQTVYVEPFINRSRDVGMEKELTSALRSEFYRRGQLRAVDLAEQADLILSGVVRSIES
                     SVASVNRRDEVLQYESVLVLDATLRRREPNEILWRGQGLRLAELHGGSRAAVVTTSSE
                     FRNAPLNSSDVRSLTDIQLTEGESRDVRQRLMERFAKELHQRIMEMF"
     gene            complement(2347..2619)
                     /gene="rpsT"
                     /locus_tag="VE170_18455"
     CDS             complement(2347..2619)
                     /gene="rpsT"
                     /locus_tag="VE170_18455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYJ17484.1"
                     /translation="MADHPSVEKRYRQSVKRRTRNIEIKSKLRTLVKKARLAIEAKNH
                     EAATSQIQSVNKALGKAVSKGIVKKNTASRWLSRLSRSANRSKSAN"
     gene            3294..3515
                     /locus_tag="VE170_18460"
     CDS             3294..3515
                     /locus_tag="VE170_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17485.1"
                     /translation="MMGKKFYSMSAIALFAFTLTVIGCGKKEEPAPPPPPPAPAEPAA
                     PAVDQKAAEPMKDAAPPPAEEKKEEAKTK"
     gene            3916..4626
                     /gene="tatC"
                     /locus_tag="VE170_18465"
     CDS             3916..4626
                     /gene="tatC"
                     /locus_tag="VE170_18465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYJ17486.1"
                     /translation="MTLTGHLEELRWCLLRSVIAISVSSAVCYFFSNAIFAYMVAPLR
                     AHLQPGQGLIGTGVTEAFFSEIKVAIAAGVLLSSPYIFFQIWHFIAPGLSGGEKKLVL
                     PFVACATLFFLGGSYFCYRIVLPVAFKYFVEQYNVMGVTPAIRIGEYFTFFFRMVLAF
                     GVTFELPVFTFFLVRLGLWNYRLMIGSFRYAIIAIFVIAAMLTPTPDIINQSLLALPM
                     LALYVLSIGVAYVWRKTD"
     gene            4658..>5262
                     /locus_tag="VE170_18470"
     CDS             4658..>5262
                     /locus_tag="VE170_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HYJ17487.1"
                     /translation="MISFVIVISCLSLAFAVYLARQVLSKDTGTQKMRDISDAIKEGA
                     EAFMSRQNRTIIILSVVLAVLIFVLYAFVRTPSKADQVPPMETAFWTTLSFVLGAACS
                     LIAGYVGMWVSIRANIRTASAVRSSLNEALQIAMRGGAVSGLFVVAMSLLGVGGLYAL
                     LDALGHDPQKIPFTIVGYGFGASFVALFAQLGGGIYTKAADV"
BASE COUNT         1093 a         1350 c         1543 g         1276 t
ORIGIN      
        1 tgctgatgga atacggcacc ggcgcggtta tggcggtgcc ggctcatgat cagcgcgatt
       61 tcgaattcgc caaaaaatat tcgctgccga tccgtatggt cattcaagcc caagataaaa
      121 acttgaccgc cgacggcttg acagaggcct acgttgacga aggcgtgatg cgcgatagcg
      181 gcgagttttc gggcacggca aacatcgccg gcaaggagat gatcgccgcc tatgccgaag
      241 cccacggctt tggcaaaccg gcgattcgct ttcgcctgcg cgattggggc gtgtcgcgtc
      301 aacgttactg gggcacgccg atcccgatta tttactgcgc caaatgcggt gtcgtgccgg
      361 tgcctgaaga tcgtttgccg gtggagctgc ccaaggaagt tccgttctcg ggtcaaggcg
      421 gttcgccgct ccaagagagc gcggcgtttc ttcgggttga ttgcccgcag tgcggcggcg
      481 acgcgcggcg cgagaccgat accatggaca ccttcgtcga ttcgtcctgg tattatttgc
      541 gctatacgtc aaccgactcg acaaccgcgc cggtggacgt cagccggcaa cgttactgga
      601 tggcggtgga tcagtatatc ggcggcgtcg agcatgcggt gctgcatctg ctctacgcgc
      661 gctttttcac caaggccttg cgtgacatcg gtttgaccca tgtcgatgag ccgttcgcca
      721 acctgcttac ccagggaatg gtttccaaag aaacctacaa gtgcgccgag catggctggc
      781 tatttcccaa cgagttgctc cggacggaag acggttggcg gtgtccgcgc tgccgccgag
      841 cggtggacaa gggccgggtc gaaaagatgt caaagtcgaa aaagaacatc atcgacccgg
      901 aagatttgat cgccgcctat ggcgccgata ccgccagact ttttacctta tttgccgcgc
      961 cgccggaaaa agatctggaa tggagcgacc agggcgtcga aggcgcgtac cgctttctcg
     1021 gccgcctgtg gcgcttggtg taccaacagg gcgacctatg gtccgctgcc gatggcggcg
     1081 cggccggcga aaaacccaac gccgaactgc gcgacctacg ccggcagatt catcgaacca
     1141 ttaaaaaagt ttccgacgat atcgacggca gatttcattt caataccgcc atcgccgcga
     1201 tcatggagct gtttaatgcc ctcagcgccg tgtctcaaga cccggccaaa agagccgctg
     1261 gagccgccgt cctgcgtgag gggctggagt cgatcatgct gttgttggcg cctttcgtac
     1321 cccatgtggc cagcgagcta tgggagcagt cgggccatcg cgagcgtctc gaccaggtgc
     1381 cctggccgac ttattcggaa gccgcgctcg aagaggagca gttgctaatc gtcgtccaag
     1441 taaacggcaa agtccgcggc aagatgaccg tgccggccgg gattacccag gagcggatcg
     1501 aagccagcgc gatggccgat gccagagtaa tgagtttttg cgacggtaag aaaatcaaac
     1561 gcgtggtcta cgtgccacgc cggttggtta atatcgtggt ggagggatga gcatgttaag
     1621 gatggtaaat tggtcggtcg gaaaggcggg tagttggcgg ctggtgctgg ccggcgccgc
     1681 gctgatactt ataaacgcct gcggctacca gtgggtcggg caatcgtcgc tgctgcccaa
     1741 ggatgcccag acggtttacg tcgagccatt catcaatcgc agccgggatg tcggtatgga
     1801 aaaagagctg acttcggcgc tgcgcagtga gttctatcgg cgtggccagc tacgagcagt
     1861 tgatcttgcc gagcaagccg atctcatact tagcggcgtt gttcgttcga tcgagagttc
     1921 ggtagcctca gtgaaccggc gcgacgaagt gttgcaatat gaatcggtgc tggtcttaga
     1981 tgccacgctg aggcggcgcg agcccaatga gattctctgg cgagggcagg gtttgcgctt
     2041 ggcggaactg catggcggct cgcgtgccgc agtggtgacc acttcatccg aattccgcaa
     2101 cgccccgtta aactcgagcg acgtgcggag cttgaccgat attcaattga ctgaaggtga
     2161 gagcagggat gtccgccagc ggctaatgga gcgcttcgcc aaggagctgc atcagcggat
     2221 catggagatg ttttaaacgc gatcattgcg cgccccgtgc cgagacaccg gcttgccgac
     2281 cctgaattgc gggcaaaaaa atcggcgcaa ctcttgtcac cgccgatcga tgtttgccga
     2341 agttcgttaa tttgccgatt tactgcggtt cgccgagcgg gagaggcggg agagccaacg
     2401 cgaagcggtg tttttcttga cgataccttt gctcacggct ttgccgaggg ctttgttaac
     2461 actctggatc tgactcgtcg cggcctcgtg attttttgcc tcaatggcga gccgggcttt
     2521 tttcaccagc gtgcgcaatt tcgacttgat ttcgatgttg cgcgtgcgcc gtttaacact
     2581 ctgccggtaa cgtttttcga ctgatggatg atctgccaag gaatgcctcc aatagattta
     2641 cgtggtcttt tgtaacatcg gctaccctga gagtcaacgc gatatccgtc tgtacaaaca
     2701 gagatcacaa tgcggcggca ggttcatgag aatgcttact caagaggtac tcgtgatcag
     2761 cgagttgccg agaaatgtaa ttgcccatgg ggttagcgcc cgagtaggga ttcatgtgtg
     2821 gtctacggct ggctaagtta tctaccggca tttgcctagg tagtcctacg ggactcccaa
     2881 attctcgtgg gacaaaaatt actcagaaag ccaaataact gatttaatgg ctaatatatc
     2941 gccgggaatt tgagcaaaga gctgcgcgag gctatttctc gcgtgcgcgt tggaagtagt
     3001 ttggtagtta cccacatgat attcacaacc gttgtcgaca tcgaggggaa atgcacgaaa
     3061 cgggcaaata aatctttcgt catttatgct ccatcgctaa acagttgctc ttgctcttta
     3121 tcatccatga cgatgccgcg cactggtgtt tgaggttctt gactttcttg ccttgaaact
     3181 cctttccacc aacgatatgt ccgcggatta cttgaaactc ctcgccactt atgtaataaa
     3241 aattgactga tcctagtaaa tcagggatgt gtaacgtgaa agggggtgaa tcaatgatgg
     3301 ggaagaaatt ttattcaatg tcggccattg ctctgttcgc ttttacttta acggttatcg
     3361 gatgtggtaa aaaagaagaa cccgctccgc ctccaccacc gcctgcgccg gctgaaccgg
     3421 ccgctcctgc ggttgatcag aaagcagctg agcctatgaa ggatgcagcg ccgccaccag
     3481 ccgaagagaa aaaagaggaa gcaaaaacca agtaaagatg tcaatcacgg gaagggggtg
     3541 aatcaaagat gggaaagaat ttagtttcat tgtcaggtgt taccctgttc gcacttactt
     3601 tagcgttggc agggtgtgag aagaaggaaa cagtagtgac tccgccgccg ccgccgccgg
     3661 caccagcagc accagcgcca gcagggccag agggggcacc tggggctcct ggagctcccg
     3721 gagcgcctgg ggctcccggt gcgcctgggg cacccgctcc ggatgcgcca atggagaaga
     3781 aagaagaagg gaaaaccaag taaggaattt tagccttacg ctggttgatg ctaccgaaaa
     3841 aggggaaggt tttaatacct tccccttttt tgcaatttcg gtgttcagtt ctgacgcgtg
     3901 gcgatccaag ctgatatgac tcttacgggg cacctcgagg agctccgttg gtgtttactt
     3961 agatctgtga ttgcgatttc cgtcagctcg gcggtttgtt acttcttttc taacgcaatc
     4021 tttgcctata tggttgcgcc actgcgcgcc catttgcagc cggggcaggg cttgattgga
     4081 accggcgtca ccgaagcctt ttttagcgag attaaagtcg ccattgcggc gggggtgctg
     4141 ctttccagcc cctatatttt ttttcaaatc tggcacttta tcgcgccggg tttatccggc
     4201 ggcgaaaaga agctcgtgct gccgttcgtg gcttgcgcga ccttgttttt tcttggcggg
     4261 agttattttt gctatcggat cgttctcccc gtggctttta aatactttgt cgagcagtac
     4321 aacgttatgg gagtaacgcc ggcaatcaga atcggcgagt attttacatt tttttttcgc
     4381 atggtcttgg cattcggtgt gacctttgag ctgccggtct ttactttttt tctggtgcgg
     4441 ttggggctat ggaattatcg cttgatgatc ggctcgtttc gttacgccat tatcgccatt
     4501 ttcgtgatcg cggcgatgct gacgccgaca ccggatatta taaatcagag tttattggcg
     4561 ttgccgatgc tagcgttata cgtattgagt atcggcgtcg cctatgtttg gcggaaaacg
     4621 gattgaagta agtcgtttca agggatgagg aggagtcatg atttcatttg ttatagtgat
     4681 tagttgtttg tcgctggctt ttgccgtgta cctggcccgc caggtgctga gcaaggacac
     4741 gggcacgcaa aagatgcggg acatctcgga cgccatcaaa gagggcgctg aggccttcat
     4801 gtcgcggcaa aaccgcacca ttattatttt atccgtggtt ttggcggtct tgatttttgt
     4861 tctctacgcc ttcgtgcgca cgccgagcaa ggctgaccag gtgccgccaa tggaaacggc
     4921 tttttggaca acgctctctt ttgtgctcgg cgcggcttgt tcgctgatcg cgggctatgt
     4981 cggcatgtgg gtttcgattc gcgctaatat acgcacggct tctgcggttc gctccagtct
     5041 caacgaagcg ttgcagatcg ccatgcgcgg cggcgcggtt tccggcttgt tcgtcgtcgc
     5101 catgagcctc ctcggcgtcg gcggtttgta cgcattgctc gacgcgctcg gacatgaccc
     5161 gcaaaaaatt ccattcacca tcgtcggtta tggcttcggc gcgtcgttcg tcgcgctttt
     5221 cgcccaattg ggcggcggca tttacaccaa ggcggccgat gt
//
LOCUS       DASQKS010000714         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_395596, whole genome shotgun sequence.
ACCESSION   DASQKS010000714 DASQKS010000000
VERSION     DASQKS010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395596"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VE170_18475"
     CDS             <1..847
                     /locus_tag="VE170_18475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HYJ17488.1"
                     /translation="FLFIMIGARQPRDISRNLKIIEKRNVRVIQSEILGIDPIRQEIS
                     LEKEKINYDYLITSLGLQTRPDLVPGFAESSQHPWEMESAIRLRASLESFRGGRVVVG
                     VPLGPYRCPPAPYEAQWMLDGYFRQKGIRNRVQIDYFTREAEPTGEAHDPTVWMNAES
                     KGRGIKQHYEFVVRSIDPEKKVVQGLYNYNIAYDLLVMVPPHRPAQVLLDSGLAESET
                     GIRVDYDTLQTKWDNVYAIGDCADMPASKAGGVAHQEADVLAHNLAVEISGKGRPVDL
                     WLHTI"
     gene            complement(1415..2917)
                     /locus_tag="VE170_18480"
     CDS             complement(1415..2917)
                     /locus_tag="VE170_18480"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYJ17489.1"
                     /translation="MPDNNRNHWDKEVDVLVVGFGAAGGISAITAHDAGAKVLVIEKM
                     PHPGGISILSGGGVAFAHNAEGAFQYLRRTCNGTTPDDILRKMAEEMVGMIDWVTELA
                     KVSGTEPSKTETRGHGTYPFPGTNEIDSIKLKDFQAYDEFPWAKGLRGGARLFKVVYD
                     NVNQRNIEVMVSTPAKELILNPDGEVIGLVAEHNGKTIRIKANKGVILCCGGYENDDA
                     LKLQYFQAQPVYPVYLGNTGDGLKMAQKAGAGLWHMWHFHGGYGFKFAELPFAIRHVW
                     AGPRNENRKMIWIGVDKTGKRFMDEYPPAPQDTGTRALEYYDADIQDYPRIPCYLIFD
                     EEGRKLGPIGIPIVNDERYDASWSSDNLAEIDKGWIKKGNSLEDIAAQIDVNPKVLRA
                     TVERWNELCANGQDEDHKRPPKTMMPIKTGPFYVMEAWPIVNNTQGGPEHDVNQRVLD
                     PMKRPIRRLYVAGEISSIYGHLYLEAGNITECFVAGKIAGQNAAAEQAWS"
BASE COUNT          608 a          880 c          914 g          729 t
ORIGIN      
        1 gtttctgttc atcatgatcg gcgcgcgcca accccgcgat atcagccgga atttgaaaat
       61 tatcgagaaa cgcaacgtta gagtgattca gtcggagatc ctgggcatcg acccgatccg
      121 ccaggaaatt tcgctggaaa aggaaaagat caattacgat tacttgatca cttcgctcgg
      181 cttgcagacc cgtcccgact tggtgccggg ctttgccgag agctcgcagc atccctggga
      241 aatggaatcg gccatacgtt tgcgtgcgag cctggagtca tttcgcggcg gcagggtagt
      301 cgtcggtgtg ccgctcggac cgtaccgctg cccaccggcg ccctatgaag cgcagtggat
      361 gcttgacggc tatttccgtc aaaagggcat ccgcaaccgc gttcagatcg attacttcac
      421 ccgcgaggcg gaacccaccg gcgaagcgca cgatccgacg gtgtggatga acgccgaaag
      481 caaagggcgc ggcatcaaac agcactacga gtttgtcgtc cgctcaatcg atccggagaa
      541 aaaggtcgtg cagggtttgt acaattacaa tattgcctac gatctgctgg tcatggtgcc
      601 gccgcaccgg ccggcgcagg tgctgctcga tagcggtctg gccgagagcg aaaccggtat
      661 ccgcgtcgac tacgatacgc tgcagactaa atgggacaac gtttacgcca ttggcgattg
      721 cgccgacatg ccggcgtcca aggccggcgg cgtggctcat caagaagctg acgtcctggc
      781 gcataatctc gccgtcgaga tcagcggaaa agggcgaccc gtggacctct ggctgcacac
      841 catatgacta ctcgccttcg gcgctggacg cgccgccgca aaccgaacca cttatcccac
      901 cggctggccg cccttcggtg tcaccgatcc gcgcacctgg gggccatcta ggatgattta
      961 ctgggcgaag gtcggcttcg agaaatggtg gctgtggcgc tatttctaaa agtttaacgg
     1021 aatcgtctgc ggtcgaaacg atctgcttat ggataacatg actttccacc actaaggcac
     1081 gaggaacgcg acgttttcgg ataactaaga ttccgaactt cgtgcccttc gtgcctcctt
     1141 agaaaatacc tgtaaggtga cggtcgccaa aatgaacagg atgcctgggt ttcgtcatac
     1201 ccgcgaaagc ggtatccagg gtaattgggc gcagatcaaa cctggtttcc cacgtgcgcg
     1261 ggaatgaccc gtcgagtcga ctgcctatgg agcgaatccc atgcattttc atattctgta
     1321 gacgagcgta agaccatgat tcacttcgtg gtgaagacag tctttgcttt gtcgatcgcg
     1381 gcgtgatcgc gccgctctat ttacaatcgc ccgctcagga ccacgcttgc tcggccgcgg
     1441 cattctgccc ggcaatcttt cccgcgacaa aacattcggt gatgttgccg gcctccagat
     1501 agagatggcc gtagatcgag ctgatctcgc cggcgacgta gaggcgccga attgggcgct
     1561 tcatcggatc gagcacgcgt tgattgacgt cgtgctcggg gccgccttgg gtgttgttga
     1621 cgatcggcca ggcttccatg acgtagaacg ggccggtctt gattggcatc atggtcttcg
     1681 gcggccgttt gtggtcctcg tcctggccat tggcgcagag ttcattccac cgttccaccg
     1741 tggctctcag cactttggga ttgacgtcga tctgggcggc gatgtcttcc agcgagttgc
     1801 cttttttgat ccaacctttg tcgatctccg cgagattgtc cgagctccaa ctggcgtcat
     1861 aacgctcgtc attgacgatg ggaatgccga tggggcccaa cttgcggcct tcctcgtcga
     1921 agatcagata gcaggggatg cgcggatagt cctgaatatc ggcgtcgtag tattccagcg
     1981 cccgggtgcc ggtgtcctgg ggcgcgggcg ggtactcgtc catgaagcgc ttgccagttt
     2041 tgtcgacgcc gatccaaatc attttgcggt tttcgtttct cggtccggcc cagacatggc
     2101 ggatggcgaa gggcagttcg gcaaatttga agccgtagcc accgtggaag tgccacatat
     2161 gccagaggcc ggcgccggct ttctgcgcca tcttcaagcc gtcgccggtg ttgccgagat
     2221 aaaccggata gaccggttgg gcttggaagt attgcagctt caaagcgtcg tcgttttcgt
     2281 agccaccgca gcaaagtatg acgcccttat tcgccttgat acgaatggtc ttgccgttgt
     2341 gctcggcgac caggccaatc acttcgccgt ccgggttcaa aatcaattct ttcgccggtg
     2401 tcgagaccat cacttcgatg ttgcgctggt tgacattgtc gtagacgact ttgaacagcc
     2461 gcgcgccgcc gcgcagtccc ttggcccaag ggaattcgtc gtaggcttgg aagtctttga
     2521 gcttgatcga gtcgatctcg ttggtgccgg gaaatggata ggtgccgtgg ccgcgggtct
     2581 cggtcttgga cggttcggtg ccgctgactt tagcgagctc ggtgacccag tcgatcatgc
     2641 cgaccatctc ttcggccatc ttgcgcagga tgtcgtccgg cgtggtgccg ttgcaagtcc
     2701 gtcgcaaata ttggaaagcg ccttcggcgt tatgcgcgaa ggcgacgccg ccgccggaga
     2761 gaatcgagat gccgccgggg tggggcattt tttcgatcac taagactttc gcgccggcgt
     2821 cgtgggcggt gatggcggag attcctcccg ccgcgccgaa gccaacgacc agcacgtcga
     2881 cttctttgtc ccaatgattc cggttgttgt ctggcattgt tgtaccttcc ttggttttcg
     2941 tggtgaaatg ctgcgcagtt gttccgagta tctatgactc aaatacttca ccagtccaat
     3001 attctcacag tcgctcttcc aaccaatcca acatgaacgg caaattatgc gcccagtaat
     3061 cgtggctgca atgaattcgc ccgccgccgt ccgcttcgtc gtagattttt aatgttttgt
     3121 cggtggcgcc g
//
LOCUS       DASQKS010000715         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2084717, whole genome shotgun sequence.
ACCESSION   DASQKS010000715 DASQKS010000000
VERSION     DASQKS010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084717"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VE170_18485"
     CDS             <1..1323
                     /locus_tag="VE170_18485"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HYJ17490.1"
                     /translation="TKREVINESQILLTPYVDDTRIDVDALGRFINEQYKIAKIGREQ
                     VDTGALILTGVAVRRRNARAIAELFAQEAGKFVAVSAGDGLEATMAAHGSGAVAHSAK
                     MGGVVLNIDIGGGTSKFAICNNGKVQEVSAIDIGARLLAFDKDGAVVRIEEAARKHAG
                     WAGFSVAMGEKIPQENLRKMVSGMVDKLFAMLNPKAIDADTKVLLRLAPLSYTGEIDC
                     VMFSGGVSEFIYNRAKTSFGDLGPLIADEVHKRAGSLGLLMMEPNARIRATVIGASQY
                     TVQVSGNTIFILPDDAVPVRNIAVVAPEFPLGQEEFTKDAVRDALLSALRRLDMLHGR
                     QPVAVAFHWDGSATFARLQAFCSGVAEGLKDILAKGHPLVLVNDGDIGGIIGLHCQEE
                     LKLDSAIISIDGIALSDFDFIDVGALIQSSGAVPVVIKSLIFPASPEQ"
     gene            complement(1352..3082)
                     /locus_tag="VE170_18490"
     CDS             complement(1352..3082)
                     /locus_tag="VE170_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HYJ17491.1"
                     /translation="MSQLEPSGANSKWLPKLFLCLQQYSRQDLVADGVAGLTVGLVAL
                     PLAMAFGIASGVSPQAGLYTAVVAGFLISALGGSRVQIGGPTGAFVVIVAGIIAKFGL
                     SGLFMVTLMAGVLLIAMGITGFGAAVKFIPRPVTIGFTNGIALLIASTQLKDFLGLMT
                     PTVPSDFIHRMKFLAQYLSSLDGYTVTIATASLAVILYAPRLIKKIPGSIVALFLGTC
                     IVAVFDLPIATIGSKFGGIPLGLPSLAIPEFRADLIIPLLPSAFTVAILAAVESLLSA
                     VVADGMSGDKHNSNVELIAQGIANLVSPLVGGIPATGAIARTATNIRSGAKTPVAGLV
                     HALTLLLILLVAAPLARFIPLATLAAVMFVVAYNMSEWREIGVILKLSNADKAVWLTT
                     FALTVLADLTVAVEVGMVLAALLYIYRVSQTTTVATITNELIENGRPHILQDKCMPDY
                     VTILRIRGPFLFGTTDKLAEETPDVSRFAPVVILRLRHMTAIDATGLHAIELFAKRVK
                     QSGRVLLLCGARDQPAEFIEQSDFLEHFGRDNILPHVQAALDRAQEIIEGFDGVGRDV
                     AHDYSLHAPV"
     gene            complement(3079..3426)
                     /locus_tag="VE170_18495"
     CDS             complement(3079..3426)
                     /locus_tag="VE170_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYJ17492.1"
                     /translation="MPDTLRQFKASIFQALAHPTRIAIIEALREEELSAGTIIERLAI
                     EQANASQHLSILRTRQIVISRKEGNQVFYSLRDPLLNELMDIMRRYFQSHVEEAAAML
                     REIGHAEKENLQK"
     gene            3792..5078
                     /locus_tag="VE170_18500"
     CDS             3792..5078
                     /locus_tag="VE170_18500"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYJ17493.1"
                     /translation="MELELLDYAVREIAWSERTLLSGTTLSLCRSDLEKQIDDLSHGI
                     KVDFALARPGEAKRIVHVLDTVMPIAKVSAKGSTFPGFEGSTALAGTGRTVRIGNLLI
                     TVAGRFPHIDELTPIEKQREGILDMSGVGAPYSYGSDYFHLVLSLTADASVSNAGFDH
                     ALRLMALRCARFIAQAQKHNAAPQHRRVALTSVAAGLPKVVLIYQVQSQMRCARTFYY
                     GEEISRTLPTLVHPAEFFDGAVVSGNFKSERKVPTSLHCDNPFIWELLNRHGKVMNFC
                     GVILSRGYNDSFEQKKKMGLWVARLAKNLGADGALAFMEGTGNGTVDFMQTVKACEDE
                     GIKTAAVLHESNGPKGYERPLVDHPQEADGMISRGNVSEKIYIPPLASVIGGPEIDLH
                     LKARHDAREPFLFDPTIFFGSYSKMGMSGFRAAYET"
BASE COUNT         1220 a         1480 c         1428 g         1094 t
ORIGIN      
        1 accaagcggg aggtaattaa tgaatcgcaa atcctcctga ctccctacgt cgatgacacg
       61 agaatcgatg tcgatgcctt gggccggttc atcaacgagc aatataagat agcgaagatc
      121 ggccgcgaac aagtcgatac cggcgcattg atccttaccg gcgtcgccgt gcgccggcgc
      181 aatgcgcgcg ccattgccga actgttcgct caagaagccg ggaaattcgt agcggtcagc
      241 gccggcgacg gcctcgaagc gaccatggcc gcccacggtt ccggcgccgt ggcccactcg
      301 gcaaaaatgg gcggcgtggt tttgaacatc gacatcggcg gcggcactag caagtttgcc
      361 atctgcaaca acggcaaagt ccaggaagtc tccgccatcg acatcggcgc ccgcctgctc
      421 gctttcgaca aagacggcgc cgttgtgcgc atcgaggaag cggcccgcaa gcacgccggc
      481 tgggccggct tctcagtggc catgggcgaa aaaatccctc aagagaattt gcgcaaaatg
      541 gtttccggca tggtcgacaa actgttcgcc atgttgaatc cgaaggccat cgacgccgac
      601 accaaagtgc tgttgcgcct ggcgccgttg agctacaccg gcgagatcga ttgcgtgatg
      661 ttttccggcg gcgtgtcgga atttatttac aatcgcgcca agaccagctt cggcgacctg
      721 gggcctttga tcgccgatga agtgcacaag cgcgccggta gcttgggact gctgatgatg
      781 gaacccaacg cgcgcatccg cgccaccgtc atcggcgcat cccaatacac ggtgcaagtg
      841 agcggcaaca ccatcttcat cctgcccgac gacgccgttc cggtgcgtaa catcgccgtg
      901 gtcgcgcccg aattcccgct gggccaagaa gaattcacca aggatgcggt gcgcgacgca
      961 ctgctcagcg cgctgcgccg cctcgatatg ctgcatggcc gccagccggt cgccgtagcg
     1021 tttcattggg acggctcggc gaccttcgcc cgcctgcaag cgttttgcag cggcgtggcc
     1081 gagggtctca aagacattct cgccaaaggc catccactgg ttttagtcaa cgacggcgac
     1141 atcggcggca tcatcggcct gcactgccag gaagagctga agctggacag cgcgatcatc
     1201 tccatcgacg gcatcgcgct cagcgacttc gacttcatcg atgtcggcgc gctcattcaa
     1261 tcttccggcg cggtgccggt ggtcatcaag tcgctgatct tcccggcatc gccggagcag
     1321 tagcccgcat cgcaagtttt cgctgtcgta tttaaaccgg agcgtggagt gagtagtcat
     1381 gggcaacatc ccggccaact ccatcaaacc cttcgatgat ttcctgggcc cggtcgagcg
     1441 cggcttggac atggggcaga atgttgtcgc ggccaaagtg ttcgagaaaa tcagattgct
     1501 cgataaactc cgcaggctga tcccgcgctc cacatagcaa cagaactcta cccgattgtt
     1561 tgactcgctt ggcaaacagt tcaatggcgt ggagacccgt agcatctatg gcggtcatgt
     1621 gacgcaagcg tagaataact accggagcaa agcgggacac gtccggagtc tcctccgcca
     1681 gcttatccgt ggtgccaaat aggaaaggac cgcgtatccg caaaattgtc acatagtcgg
     1741 gcatgcattt gtcttgcagg atgtgcggtc ggccattttc gattaattca ttagtaatag
     1801 tcgccaccgt agtcgtttgc gaaactcgat agatataaag caaggcggcg agtaccatac
     1861 caacttcgac cgcaaccgtg aggtcggcaa gcacagtgag cgcgaaagtc gttaaccaca
     1921 ccgccttgtc tgcgttcgag agcttcagaa tgacgccgat ctctctccac tcgctcatat
     1981 tataggcgac gacaaacatg accgcagcaa gagtcgccag cgggataaaa cgcgccagcg
     2041 gcgctgccac taacaaaatc aacagcaagg tgagcgcatg taccagtccc gccaccggcg
     2101 tcttagcgcc ggagcgtatg ttggtcgccg tccgcgcgat cgctcccgtg gcaggtatac
     2161 cgcccacgag gggactcacc aagttagcaa tcccctgggc aatcaactcg acgttggaat
     2221 tgtgtttgtc tccgctcatg ccgtccgcca cgaccgccga aagtaggctt tcgacagcgg
     2281 cgagaatcgc cacagtgaaa gcagaaggca aaagcggaat gattagatca gcgcgaaact
     2341 cgggaatcgc caacgacggc aggccgaggg ggatgccacc gaacttgctg ccgatcgtgg
     2401 caatcggcaa gtcaaacacg gcgacgatgc aagtgcctag aaagagcgca acgatcgatc
     2461 cgggaatttt ttttatcagg cgcggtgcat aaagaatgac agcgagcgat gccgttgcaa
     2521 tcgttaccgt gtacccgtcg agcgatgaaa gatactgcgc caggaatttc attcggtgaa
     2581 taaagtcact gggtaccgtt ggggtcataa gccccaggaa atctttgagc tgggtcgaag
     2641 caataagcaa agcgatgcca ttcgtaaagc cgatggtgac cggtctggga atgaatttga
     2701 ccgctgcgcc aaatccggtg atccccatcg caatgagcaa aacaccagcc atcaaagtca
     2761 ccatgaacag gcccgaaagt ccgaatttgg cgatgatgcc ggcaactatg acaacaaaag
     2821 cgcccgtagg gccgccgatc tgtacccgtg atccgcctag tgcagaaatc aaaaatccgg
     2881 cgacgaccgc cgtgtagaga ccggcctgag ggctcacgcc ggaagcaatg ccgaaggcca
     2941 tcgccaacgg taaggccacc aggccgaccg ttaaaccggc cacgccatcg gcaaccaagt
     3001 cttgacgcga gtactgctgc agacacagaa acagtttggg cagccattta ctgtttgccc
     3061 ccgatggttc taattgactc acttttgcaa gttctccttt tcggcgtggc cgatctccct
     3121 gagcatggct gccgcctctt cgacatggct ctgaaaatag cgccgcataa tatccatcag
     3181 ctcattcagt aacggatcac gtaaagagta gaacacctgg tttccttctt tacggctgat
     3241 aacgatttgc cgagtgcgta agatcgagag gtgttgcgac gcgttggctt gttcgattgc
     3301 caatcgctcg atgatggttc cagcggacaa ctcttcctcc cgcaatgctt caattatcgc
     3361 gatccgcgtg ggatgggcga gggcttgaaa aatactcgct ttaaattgcc ttaatgtgtc
     3421 tggcatacga aatctccgat gaaattgaat gtagacctgt attgtaataa cagtttatta
     3481 tattcgcaat atcgaatata cttatataaa tgtcgctagc ccctgaacag atcttcaaat
     3541 tagatattcg tttgtttttt caatagctta taggcagctc gattagttct ctttaggcgc
     3601 tacgaaaata tttcatctcg gtaatttccg aatgacggga cgggtcgacg cgcgatatta
     3661 acgcacgaag taggtatcca gtcgccaggt gtctcccgaa tagcaattcg acactccatg
     3721 aattgttcat tgacaccaag cttactgcca cgataaggat cggtcgttcc gcctaccgaa
     3781 agggatatgc gatggaactg gaactacttg attacgctgt ccgtgaaatt gcctggagcg
     3841 agcgcacgct gcttagcggt accaccctct cactctgccg gagcgacctt gaaaagcaaa
     3901 tcgacgatct tagtcacggc atcaaagttg acttcgcatt ggctcggccc ggcgaagcca
     3961 agcggattgt ccacgtcctc gatacggtca tgccgatcgc caaagtctct gctaaaggct
     4021 cgacttttcc gggattcgaa ggttccactg ccctcgcggg gacgggtaga accgtgcgca
     4081 tcggcaacct gctcatcacc gtcgctggtc gcttcccgca catcgatgaa ttgacgccca
     4141 tcgagaaaca acgcgagggg attctcgaca tgagcggcgt cggcgctccc tactcctacg
     4201 gctcggatta ttttcatctg gtgctgtcac tcactgccga tgcttcggtc tccaacgccg
     4261 gcttcgatca cgcgctgcgg ctcatggcgt tgcgctgcgc gcgctttatt gcgcaagcgc
     4321 aaaagcacaa cgccgcgccg cagcaccgca gggtcgcact cacttcggtc gccgcgggcc
     4381 tgcccaaagt ggtcttgatc tatcaagttc aaagccaaat gcgttgcgct cggacttttt
     4441 actacggcga agaaatttcg cgaaccttgc cgaccttggt tcatccggcg gaatttttcg
     4501 acggcgcggt ggtcagcggc aacttcaaga gcgaacgcaa agtgccgact tcgctgcatt
     4561 gcgacaaccc gtttatatgg gaattgctca accgccacgg caaggtcatg aacttttgcg
     4621 gcgtgatcct ttcgcgcggt tataacgaca gcttcgagca gaagaaaaaa atgggcctgt
     4681 gggtggcgcg gctggcaaaa aatttgggcg ccgacggcgc gctggctttc atggaaggca
     4741 ccggcaacgg caccgtcgat ttcatgcaaa ccgtaaaagc atgcgaagat gaagggatca
     4801 agaccgctgc ggtgttgcat gaatcgaacg gccccaaggg ctatgagcgg ccgctcgtcg
     4861 atcatccgca agaggccgac ggcatgatca gccgcggcaa tgtttcagaa aaaatttata
     4921 taccgccgct cgccagcgtc atcggcgggc cggagatcga tctccacttg aaagcgcgcc
     4981 atgacgcgcg tgagcctttt ctcttcgacc cgacgatttt tttcgggtcc tatagcaaaa
     5041 tgggcatgag cggatttcgc gcggcgtatg aaacatagtg gctgtcaggt cgcaagagtt
     5101 gtctcgcgcc atcagcgcaa agaacgccaa acgcggcatt ttatctgcta atctttgcgt
     5161 gctttgcacg aaaaatgttc gtagaggaga cacgacatgc ctaaagtgct ccattatctg
     5221 aa
//
LOCUS       DASQKS010000716         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_5869268, whole genome shotgun sequence.
ACCESSION   DASQKS010000716 DASQKS010000000
VERSION     DASQKS010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5869268"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VE170_18505"
     CDS             complement(<1..591)
                     /locus_tag="VE170_18505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ17494.1"
                     /translation="MPGVFDADTHIAESEAMWSFIDKEMYPRRPVLARIPDDTWYHER
                     NAFWLIDGEIFPKPAGKASFSLITPSAQKKESGRGDIHLAVRELTDPLARLKDMDKIG
                     VEAQVIYPTLFLVYLTDDAQLDIALSRAYNRFIASACAKAPERLKWVAILPLRDIAAS
                     LEEMKWAKQQGAVGVFFRGMEGNLTLDNPQLHPIYAL"
     gene            complement(793..1788)
                     /locus_tag="VE170_18510"
     CDS             complement(793..1788)
                     /locus_tag="VE170_18510"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17495.1"
                     /translation="MKRIIWIPLLMLFFSMSIAPAKAQNRFRAVSGGFGTAIHAVLWV
                     GYEKKIFQKYGLDMEYLAIENGTVSMQTLLANETQAAFTTGALAVTSNLQGGDATIIA
                     GGINFIPNKLVTRPDIKRPEDLKGKRIAISRFGSASDYATQLALEKLNINPKDVTIIQ
                     AGGNSTRFSAIVGGSIHATLLSEPLTTMALRDHKMNALIDLAESGVPFPQNAFLVRRS
                     YLEANRAKVVNFMKGVIEGYYTLKNDKPLAIQMIKKYIRVSDEDAAIGYDYYLAKYGD
                     GVMVMPERKGIEFILGQVAKDNPKAKGQTAESLRLLDGSVLDEIRKSGFIDKFKK"
     gene            1983..2948
                     /locus_tag="VE170_18515"
     CDS             1983..2948
                     /locus_tag="VE170_18515"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17496.1"
                     /translation="MIASAAMFYSPRPSHAQEPEKLVIGHSNLRNDIAALWVPKELGL
                     FRKHGVEVTVVLITGGARMTQAMLSGSSPMAFTGATPVVSAVGGGSDSVLILGINNKL
                     TYDIWARAEIKKPEELKGKTIGISGFGSSSHVASFLMLQHFNLDEKRDRISFVAIGDE
                     PTRAQAVLAGRIDATLMDASISGQIKGRGPTYLGNLEQLGVPFVNNALVTTRSFLKER
                     PKTAEAVVKVIVEGNAFILNPANRSTVTNIVARNLRLSGDDAKHAYDDLIPKVERRPY
                     PNLDAVKAAIQIMGARNPKIAQLKTEDIVDTSILRRLEQSGFVRQ"
     gene            2977..>3575
                     /locus_tag="VE170_18520"
     CDS             2977..>3575
                     /locus_tag="VE170_18520"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYJ17497.1"
                     /translation="MIFLTNEQIEQVLDMKTCIDAMEDAYRELNELKAGYRPRIDFYV
                     PNEPHYYRWGTMEGASRKLGVFAIRMKSDMLAWENQDGFMVEDKYCMERGTYCGLIFL
                     TSTRNGEPLAIMNDGYLQHMRVGACAGLGTKYLARKDAKVMAMIGSGGMARSYTEAIK
                     HVRPLEVVRVFSPTKKNREAFAAEMTEKLDIEVIAVDSP"
BASE COUNT          789 a         1057 c          943 g          786 t
ORIGIN      
        1 caacgcatag attggatgaa gctgtggatt atcgagggtc agattgcctt ccatgccgcg
       61 aaaaaacacg cccaccgcgc cttgctgctt ggcccacttc atctcttcca gcgacgcagc
      121 gatatcgcgc agcggcagga tcgccaccca cttgagccgt tccggcgctt tcgcgcaggc
      181 gctggcgata aagcgattgt aagcacggct cagcgcaata tccagttgcg catcatcggt
      241 gaggtagacc agaaacagag tcggataaat cacctgcgcc tcgacgccga tcttatccat
      301 gtctttcaac cgcgccaaag ggtcagtcaa ctcgcgcacg gccagatgaa tatcgccgcg
      361 cccggactct ttcttctgcg ccgatggcgt aatcaaacta aagctcgcct tgcctgcagg
      421 cttggggaaa atctcgccgt cgatcaacca gaacgcgttt cgttcatggt accaagtatc
      481 atccgggatg cgcgccaaca ccggccggcg cggatacatc tctttgtcga taaaactcca
      541 catcgcttcg gactcagcga tatgggtgtc ggcgtcgaaa actccgggca tcttcagctc
      601 cttaacttcc gttcaatcgg ttcaagccgt caagcgctgc gctccgttca agccccctcc
      661 tcgatcctcc ccctcgtgtc acgagtgagg atcgaagata gtgatccgcc tttgcgacct
      721 ttgcgttctc tgcggccaat ctccgacttc gattcttctc tctgcgaccg ccgcgcctcc
      781 gcgcacctgt tattacttct taaacttgtc gataaatccg ctcttgcgga tctcatcgag
      841 cacgctgccg tccaagagcc gcaaactttc cgccgtctgc ccctttgcct tcggattatc
      901 ctttgccacc tggccaagaa tgaattcaat ccccttgcgc tccggcatga ccatcacgcc
      961 atcgccatat ttggcgagat aatagtcgta gccgatagcg gcgtcttcgt cgctgacgcg
     1021 gatatatttc ttgatcatct ggatcgccag gggtttgtcg tttttcaatg tgtaataacc
     1081 ttcgatcacg cctttcatga agttgacaac cttggcacgg ttggcttcca ggtaactgcg
     1141 ccggaccagg aaagcatttt gcgggaacgg cacgccgctt tcggcaagat cgataagtgc
     1201 gttcatcttg tgatcgcgca gcgccatggt cgtcagaggt tcggacaaga gcgtcgcgtg
     1261 aatactgcca cccacgatag ccgagaagcg cgtcgaattg ccgcccgctt gaatgatcgt
     1321 gacatccttg gggttgatgt tgagcttttc caatgccaac tgagtagcgt aatcggacgc
     1381 cgaaccgaat cgactgatgg cgatgcgttt acccttcaag tcttcaggcc gtttgatgtc
     1441 cggccgggtc accaacttgt tgggaataaa attgatgccg ccggcgatga tcgtcgcatc
     1501 gccgccctgc aaattggacg tcaccgccaa ggcgccggtg gtaaacgccg cctgagtttc
     1561 attggccaac aacgtctgca ttgacacagt gccattctcg atggcgagat attccatgtc
     1621 gagtccatat ttttgaaata tctttttctc gtagccgacc cacagcaccg catggatcgc
     1681 cgtgccaaaa ccaccgctga cggcgcggaa gcgattctgc gccttggctg gcgcaatcga
     1741 catgctgaaa aacagcatga gtagcgggat ccagatgatc cttttcacga ttgtctctcc
     1801 ctaaaattgc cggcgaaacc gttgcggtgg catatctagg atgtttgtac gccgtgagca
     1861 ggttgcgagc aagaagtaaa atggccttga tccagagtca gattgggata ttctcccagg
     1921 cgatgaagtc tgatcgcgct tggcgccgaa acgtcgcaca tcgtcgccgg cttttttgcg
     1981 tcttgattgc ctcggccgcg atgttctaca gcccgcgccc gagccacgcg caagagccgg
     2041 agaaactcgt catcggtcac tccaacctac gcaacgacat cgccgcactc tgggtaccga
     2101 aagaactcgg cctgttccgt aagcatggcg tcgaggtcac ggtggtgctg atcaccggcg
     2161 gcgcgcgcat gacccaagcc atgttgtcgg gctcctcgcc aatggcgttt accggtgcaa
     2221 ccccggtggt aagcgctgtc ggcggcggca gcgatagtgt gctgatcctc ggcatcaata
     2281 ataaacttac ctacgacatc tgggccaggg ccgagatcaa gaagcccgag gaactcaaag
     2341 gcaagaccat cggcatcagt ggtttcggca gctcttccca cgtcgcgtca tttttgatgc
     2401 tgcaacattt caatttggac gagaaacgcg accgaattag ctttgtcgcc atcggcgacg
     2461 aacccacgcg cgcccaagcg gtcttggccg ggcgcatcga cgcgaccttg atggacgcat
     2521 cgatttcggg tcagatcaaa ggccgcggac ctacttatct cggcaatctt gagcaactgg
     2581 gcgtaccctt cgtcaacaac gcactggtca ccactcggtc atttctcaag gagcggccga
     2641 aaactgctga ggcggtggtc aaggtgatag tcgaaggtaa tgcttttatc ctcaatcccg
     2701 cgaatcgctc gacggtcaca aatatcgtcg ccagaaacct gcggctttcc ggcgacgatg
     2761 ccaagcacgc ctatgacgac ttgatcccca aagttgagcg tcgcccttat cccaacctcg
     2821 acgcggtcaa ggccgccatc cagatcatgg gcgcgcgaaa ccctaaaatc gctcagctga
     2881 aaaccgaaga tatcgtcgac acgagtattc tccggcgtct cgaacaaagt ggatttgttc
     2941 gccaataaca actgctacat cggccaaagg ataattatga tttttctcac caatgaacag
     3001 atcgaacagg tgctcgacat gaagacctgt atcgacgcca tggaagatgc ttaccgggaa
     3061 ctcaatgagc tcaaagccgg ttaccggccg cgcatcgact tctatgtgcc taacgagccg
     3121 cattactatc gttggggcac catggagggc gcttcgcgca agttgggggt gtttgcgatt
     3181 cgcatgaagt cggacatgct cgcctgggag aatcaggacg gcttcatggt cgaagacaag
     3241 tattgcatgg agcgcggcac ctattgcggc ttgatctttc tcaccagcac gcgcaatggc
     3301 gagccgctgg ccatcatgaa cgacggctac ctgcagcata tgcgcgtcgg cgcctgcgcc
     3361 ggcctcggca ctaaatatct cgcccgcaaa gacgccaaag tcatggccat gatcggctcc
     3421 ggcggcatgg cgagaagtta caccgaagcg atcaaacatg ttcgaccgct cgaagtcgtg
     3481 cgcgtgttta gcccgacgaa aaagaaccgc gaggccttcg ccgccgagat gaccgagaag
     3541 ctcgatatcg aagtaatcgc cgtcgactcg ccgga
//
LOCUS       DASQKS010000717         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2093360, whole genome shotgun sequence.
ACCESSION   DASQKS010000717 DASQKS010000000
VERSION     DASQKS010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093360"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="dinB"
                     /locus_tag="VE170_18525"
     CDS             complement(<1..1095)
                     /gene="dinB"
                     /locus_tag="VE170_18525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYJ17498.1"
                     /translation="MSWFLHIDMDAFFASVEEALDPSLKGKPVIVGGRNGRGVVTSAS
                     YAARKFGVRSAMPGFQAKKLCPQGIFLPNRRRVYVEFSDKVFAILEHYSPEVHKLSID
                     EALVDLTGTERLFGPPLKTADAMIRRIEHELGLPSSAGLSTSRVAAKIAATLAKPQGL
                     VQIPAGAEADFLAPLAVETIPGVGPKTHKGMLQRGINTIGDLMARPELAARYLDLSDT
                     AAHRHSHDHSIGNETTLGQPLTTIAAMEEVLWELVEEVGCRLRQQESFARCLTVKIRY
                     SNFQTITRSRTLASPTCFDNEIFQVVSDLLRRNIAEGRAVRLLGVSASSLQISGWQEP
                     LLNRERRASFGRLYQGIDKLRRKYGDQAIGA"
     gene            complement(1254..2258)
                     /locus_tag="VE170_18530"
     CDS             complement(1254..2258)
                     /locus_tag="VE170_18530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17499.1"
                     /translation="MKRLQFFRICRAATLAVLLSLLLPASRPLSAAEAPSMRIVYNAL
                     GGSMAPLWLGQELGLFAKHGLQHSLNYLAATTSVQAIVAGSEDIGMVGNQCIDVALEG
                     ADTIYVAGNINRFVFHLYGDPAIKTVQDLKGKTVAATQAAASTDYATRIVLRRYGLVP
                     DKDVKILFTGSMPALVTTVKSGNAAAGLMSAPTTIQALELGMKPILNVTELNIPFVFV
                     GVCTTRKMIQQRPDHISRFLRAYIESLAIIRNDKETTFKAMGKFLKTDNRQVLESIYD
                     DYGSAYQRVPLMTKQEVQAVLDVVKSPKGVQARPEVFYDNSFVQKIEASGFINSLYAR
                     "
     gene            complement(2283..2756)
                     /locus_tag="VE170_18535"
     CDS             complement(2283..2756)
                     /locus_tag="VE170_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17500.1"
                     /translation="MLKSGNAAAGLINAPVIYQAQELGMKPIVNLTELNIPFIFVAVC
                     TTKKVIQQKPDAIMRYLRAYTEAISIILRDKETVLKIMAKHMKMDNRQWVEAVYDDIA
                     RVLQRTPYMTKPEVQAVLDAVRNPKGTQVKAEEFFDNSFLQKIDNSGFINALYAR"
BASE COUNT          591 a          771 c          846 g          655 t
ORIGIN      
        1 cgcgccgatg gcctggtcgc catacttccg gcgcagcttg tcgatgcctt gatacaggcg
       61 gccgaaagat gcgcgccgct cgcggttcaa caagggttcc tgccagccgg aaatttgcag
      121 cgagctggcg ctcacgccta acagacgcac cgcgcggccc tcggcgatgt tccggcgcag
      181 taagtcgctc accacctgga aaatctcgtt atcaaagcat gtgggcgagg cgagggtgcg
      241 cgatcgggta attgtctgga aattgctata gcgaattttc accgtcaagc agcgggcaaa
      301 ggactcttgc tggcgcagtc tgcagccgac ctcttcgacc agctcccaaa gcacttcctc
      361 cattgccgcg atggtcgtga gcggctggcc caaggttgtt tcattgccga tggaatgatc
      421 gtgggagtgg cggtgtgccg cagtgtcgct gagatcgaga tagcgcgcgg ccaattcagg
      481 ccgtgccatt agatcgccaa tggtgttaat gccgcgctgc agcatgccct tgtgggtctt
      541 ggggccgaca ccggggatcg tctccacggc cagcggcgcg agaaagtctg cctcggcccc
      601 ggccgggatc tggaccagcc cttggggttt tgccaaggtg gcggcaattt tggcagcgac
      661 gcggctagtc gacaaaccag ccgacgacgg cagccccaac tcatgttcga tgcgccggat
      721 cattgcatcg gcggttttga gcggcggacc gaataacctc tccgtgccgg tgaggtcgac
      781 cagcgcctcg tcgatggaca gcttgtgcac ttcaggcgag taatgttcga gtatggcgaa
      841 tactttgtcg gagaattcca catacactcg ccgacggttc ggcagaaaga tcccctgtgg
      901 gcagagtttt ttagcctgaa aacccggcat cgccgagcgc acgccgaact ttcgcgccgc
      961 gtaagacgcc gaggtgacca cgccgcggcc gttgcggccg ccgacaatca ccggtttgcc
     1021 cttcaaactc ggatcgagag cttcttccac cgaggcgaag aaggcgtcca tgtcgatgtg
     1081 gagaaaccag gacaatgtgt gggtagcctt attgaacgag cctatcgccc cacgaaggac
     1141 acacgaaggt tctttgtagg ggcgaaagat ttttcgcccc tactcggatc atccgaaccc
     1201 tttgtgccct tgattttcgt agtggtgaaa tcgagatctt tcattatttt gcctcagcgg
     1261 gcatagagcg aattgataaa gcccgaggct tcgatctttt gcacgaagga gttgtcataa
     1321 aatacttcgg gtctggcctg gacacccttg gggcttttta cgacatcgag aaccgcctgg
     1381 acttcctgtt tggtcatcaa cggcacgcgt tgataggcgc tgccgtaatc gtcgtagatg
     1441 ctttccaaga cttgccggtt atcggtcttt aaaaatttac ccatggcttt gaacgtggtt
     1501 tctttgtcgt tgcgaataat cgccagagat tcgatataag ctctgagaaa ccggctgata
     1561 tgatcgggtc tttgctggat catttttcgc gttgtgcaga cgccgacgaa gacgaaggga
     1621 atatttagct cggtaacgtt gaggatcggt ttcataccca gctcaagggc ttgaatcgtc
     1681 gtcggcgccg acatcaggcc ggcggcggcg ttgccggact tcaccgtagt caccagcgcc
     1741 ggcatgctgc cggtgaagag aatttttaca tccttgtctg gtaccaaacc gtaacggcgc
     1801 aggacgatgc gggtcgcgta gtcggtggag gcagcggctt gggtagccgc gacggttttg
     1861 cccttcagat cctgcacggt tttgatcgcc ggatcgccgt ataagtggaa gacaaagcga
     1921 ttgatgttgc cggcgacgta gatggtgtcg gcgccttcga gggcgacatc gatgcactga
     1981 ttgccgacca tgccgatgtc ctcgctaccg gcgacgatgg cctgcaccga ggtcgtcgcg
     2041 gccaagtaat tcaaactatg ttgcagacca tgtttggcga acaggcccaa ctcttggccc
     2101 aaccacagcg gcgccatgga accgccgagg gcgttataga cgatgcgcat gcttggcgct
     2161 tcggcggcac tgagcgggcg actcgctggc agcaataagc tgagcagcac ggcaagcgtc
     2221 gcggcacggc agatgcgaaa gaactggagg cgtttcattt tgttctccct gatttgcaaa
     2281 gatcaacgag cgtagagcgc attgataaag ccgctgttgt cgattttctg caagaatgag
     2341 ttgtcgaaaa actcttctgc tttgacctgt gtgcctttgg gatttctcac ggcatccaat
     2401 accgcttgga cttccggctt ggtcatgtac ggcgtgcgct gcagtacccg cgcgatgtcg
     2461 tcatagaccg cctcgaccca ctggcgattg tccatcttca tgtgtttggc catgatcttg
     2521 agcacggttt ccttgtcgcg caggataatc gagatcgctt cggtataggc tctgagataa
     2581 cgcataatcg catcgggttt ttgctgaata acttttttgg tcgtgcaaac ggcgacgaag
     2641 ataaacggaa tattcaactc ggtgagattg acgatcggct tcatgccgag ctcttgcgct
     2701 tgatagatca ccggcgcgtt gatcaagcca gccgcagcgt tgcccgattt taacaccggc
     2761 tatgtgcgcg atgaaaatga ctttgacaaa tccccaccgg cgaatcataa gcacctcccg
     2821 tgagctgtgc tggtcgggag agtaggagag cgaatgggac ctg
//
LOCUS       DASQKS010000718         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4594996, whole genome shotgun sequence.
ACCESSION   DASQKS010000718 DASQKS010000000
VERSION     DASQKS010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4594996"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(501..1832)
                     /locus_tag="VE170_18540"
     CDS             complement(501..1832)
                     /locus_tag="VE170_18540"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HYJ17501.1"
                     /translation="MIVRIPFANRVLVSLCVFAAASLISPISHADLQRYVDKPEPAFE
                     WQVKSKIGREQSGDRVYDLQFVSQIWRGEKWRHQLQVYRPADATPGNKMLLWVTGGSA
                     SAQTNALGLELARKIKAPVAFVYHIPNQPLLEGKLSEDDLIAETFVRYLKSGDDNWPL
                     PLPMVKSVVKAMDVLQAFSKQEWNASVDQFVVAGASKRGWTTWLTAAVDRRVAAIAPV
                     VIDVLNMRAQMSRQIEAFGAYSARLIAYSSRGLLPIPDTPAGNKLLDMVDPWAYRDRL
                     TMPKFIVNGTNDFYWATDALNLYWDGIPNDKWVLYVPNAGHNLRRQDRPPQEQLNDLI
                     IGLAAFTRHQINGTAMPSLRWRHENADGKLRLIIDATPAPRSARLWVARSPSSDFRTI
                     EWRQENLRVADGRVIGEVTRPEQGRVAFFGELEYEIDGLSYTLSTQMRLTQ"
     gene            2025..3644
                     /locus_tag="VE170_18545"
     CDS             2025..3644
                     /locus_tag="VE170_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17502.1"
                     /translation="MNPNKFFISVTASLILFASILIAQEKATEKLGRVQFPVSCTAAA
                     QKQFDQALGALHSFWYEEALRMFTAVTETDPGCAMGHWGIAMSIYYPLWVPPSQPTLQ
                     KGMNAIEKAKSIGAKTDREGAYIAAIEAFYKDSDKLDHRTRAGAYEKALERIHLQYSN
                     DSEAAIFYALALNATAPPTDKTYANQLKAGEILEKVFSEQPDHPGVAHYIIHSYDNPA
                     LASRGIPAAQRYTKIAPSVPHVQHMPSHIFTRIGLWQESIQSNLGAVAASKAYAAKAH
                     PEAAYYEQLHALDYLAYAYLQGAQDREAKNVLDELRAIKKVQPEAFQAAYAFAAIPAR
                     YTLERRRWSEAAALTVTPTTFPWSRFPWSEAVTHFARAMGSARSGNVANARKDIEKLE
                     SLQDALVKAKDSYWAKQVDIQRRVATAWYLRTERKNDEALQLMQSAADLEDSTDKHPV
                     TPAPIQPARELLGEMLLELGDPAQALKEFEASHRIEPNRFRGLYGAAKAAQTAGDREK
                     ARHYYQRLVSLCERADTDRPEIKEAKAYLTK"
BASE COUNT          968 a         1099 c         1135 g          834 t
ORIGIN      
        1 agttttctga cagcgaagga ggcggaaatg gagggccacc tgtttgccaa aaggagggtc
       61 gacgacggta aaccaaatct cgaatactaa cggtaagtga gcccaagaaa gccccagccg
      121 tagttgcggt ttggagtctg cactgcacct gcagaagata gggcctgctc aaggagtgga
      181 gtggggagct ttagcggctc ttttaattgc gcattcgacg atatttttcc cgcaggcgcg
      241 cacactacga acattgccgg agtttcagca aagttgcgta ttttatgtca gcgaaaacag
      301 ggcgcagaat gccaagccaa gcgcgccccg ttaactcgca aatcaaccgg cgacgaacgg
      361 atcgtgggcg aatttcgccc aaaggttcct tagggatagg gcagacagga gggaaactgc
      421 tgacaggcaa tcgagacgtt cgatgcaaaa gccggagcgc caaacaactt ctttcgcaaa
      481 cacgagtacg cgcagtccgc ttattgcgtc agacgcattt gcgtcgacaa ggtgtagctc
      541 aagccatcga tttcgtattc cagctcgccg aaaaaggcca cacggccttg ctccgggcgc
      601 gtgacttcgc cgatgacccg gccgtcggcg acgcgcagat tttcctggcg ccattcgatc
      661 gtgcggaaat cgctgctcgg tgagcgggcg acccatagtc gcgcgctgcg cggcgccggc
      721 gtcgcgtcga tgatcaagcg cagtttgccg tcggcgtttt cgtggcgcca gcgtagactc
      781 ggcatcgccg taccgttgat ttgatggcgc gtgaaggcgg ccaggccgat gatcaagtcg
      841 ttcaattgct cttgcggcgg gcggtcttgg cgccgcagat tatgtcccgc gttgggaacg
      901 tagagcaccc atttgtcgtt cgggatgcca tcccaatata gattcaacgc gtcggtggcc
      961 cagtaaaaat cgttggtgcc gttgacgatg aacttgggca tcgtcagccg gtcgcgatag
     1021 gcccagggat cgaccatatc caaaagtttg ttaccggcag gcgtatcagg aatgggtaga
     1081 agaccgcggc tgctgtaggc gatgaggcgc gcgctatagg cgccgaaagc ttcgatctgg
     1141 cgcgacattt gtgcgcgcat gttgagcacg tcgatgacca ccggcgctat ggcggcgacg
     1201 cgccgatcga ccgcggctgt cagccaggtg gtccaaccgc gcttcgaagc gccggcgacg
     1261 acaaactggt caacggacgc gttccattcc tgcttgctaa acgcctgcaa aacgtccatc
     1321 gccttgacga cgctcttcac catcggcaac ggcagcggcc agttgtcgtc cccggatttg
     1381 agataacgga caaaagtttc cgcgatcagg tcatcttcgc tcagtttgcc ctctagcagc
     1441 ggctgattag gaatgtgata aacgaaagcg accggcgcct tgatcttacg cgccagctcg
     1501 agccctagcg cgttggtttg cgccgacgcc gagccgccgg tcacccagag taacatcttg
     1561 ttgcccggtg tcgcgtcggc tggccgatag acctgcaatt ggtgacgcca tttctctccg
     1621 cgccaaattt gcgacacgaa ctgcagatca taaacccgat cgccgctctg ctcgcgtccg
     1681 atcttggact tcacctgcca ctcgaaagcg ggctcgggct tatctacgta tcgttgtagg
     1741 tcggcgtggc taattggtga aatcaaactc gcggcggcga atacgcaaag tgaaacaagg
     1801 accctgttcg caaacgggat tcgcacgatc attggttcta tttctccgcg gtgaacgttt
     1861 ggcgcacgca catattgtat cagttctttc ggtggaccga agaccaaaag atccgatttt
     1921 aattggcttg tgtttgtggc atatccatta ccggataagg taagggccac ctgcgagcga
     1981 gggcggacca gactgaatca aaaaatgatc gaaaggtaag aaccatgaac cctaataaat
     2041 tctttatctc tgtgactgcc tcgctgattt tgtttgcatc tattttaatc gcgcaggaga
     2101 aggcgacgga aaaactgggc cgagtgcaat ttccggtttc ctgcactgcg gcggcccaga
     2161 aacagttcga tcaggctctg ggcgcgctac actccttctg gtacgaggaa gcgctcagaa
     2221 tgtttactgc cgtcacggaa acggatcctg gttgtgctat gggccactgg ggaatcgcga
     2281 tgagcattta ctatcctctt tgggtgccgc cgagtcagcc gacattgcag aaggggatga
     2341 atgcaatcga aaaagccaag tcgatcggcg ccaaaacgga ccgcgaaggc gcctatattg
     2401 cggcgataga ggctttttat aaggactcgg acaagctcga tcaccgaacc cgggccggcg
     2461 cgtatgagaa agcattggag cggatccatc tccaatattc aaacgacagc gaagcggcga
     2521 tcttctacgc gcttgcgctc aacgctacgg cacctccaac cgacaaaact tacgcaaatc
     2581 agctaaaggc cggtgaaatc ctcgaaaagg ttttttccga gcagccggat catcctgggg
     2641 tggcccatta catcatccac agctatgata accccgccct ggcaagccgt ggaattcctg
     2701 cagcacaacg ctacaccaag atcgccccct cagttcctca cgtgcagcac atgccttctc
     2761 acatttttac gcggatcggt ctgtggcagg aatcgattca atcgaatctc ggtgccgtgg
     2821 ccgcatcgaa agcgtacgca gctaaggctc atcctgaagc cgcgtactac gagcaacttc
     2881 atgccttgga ctacctggca tatgcgtact tgcaaggggc tcaggatcgg gaagccaaga
     2941 atgtgctcga cgaactccgg gccatcaaaa aagtccaacc ggaagccttt caggcagcct
     3001 atgcctttgc ggcgattccg gcgcgctata cactcgagag gcgtcgctgg tctgaggccg
     3061 cagcgttgac cgtaactcca accacgttcc catggagccg tttcccctgg tcagaggcgg
     3121 ttacgcattt cgcccgcgcc atgggatctg cacggagcgg caatgttgcg aatgctcgaa
     3181 aggacattga aaaactcgag tcgctccagg atgccttagt caaggctaag gacagctact
     3241 gggccaagca ggttgacatc cagcgccgag tggccacggc ttggtacctc cgaacagaac
     3301 gcaaaaatga cgaggcactg cagctcatgc aatccgcagc agatctggaa gattcgactg
     3361 acaaacaccc ggtcactccg gcgccgatcc agcctgcgcg cgaactgttg ggtgaaatgc
     3421 ttctcgaact cggcgatcct gctcaagcat taaaagaatt cgaggcatcg catcgaattg
     3481 aacctaaccg ctttagggga ctctatggcg cggcgaaggc tgcccaaacc gccggggacc
     3541 gtgaaaaagc ccgccattac tatcaaaggc tagtttctct ctgcgaacgg gcagacacgg
     3601 atcgtccgga gatcaaagaa gccaaggcgt atctgacgaa atagaatctt agttacaaag
     3661 gcatgaagcg ctggaacaaa cagaatggga ctgaattctc cttctgtatc tgcaagaatc
     3721 ctcgagagtg ggccattgaa aaatatgcac gaagccatgg gccgataatg aagaggcatg
     3781 gggtcgtttg cgcaaaaatt ggtgcacaaa cgagctgaca gacatggggc tcagccagtc
     3841 atcgagcgta gagatacgac gtggcggggg gtgccaaaac acttgcgagc gtcacataat
     3901 gtgatgagag tcaaagaatg cgattgagca aaccctccta gagtcctaac ggtgagattt
     3961 agaccccacc ctggaacaca gttgagacga aatttattta ctttaaccga attgttggtc
     4021 gagccaatgg ctctcg
//
LOCUS       DASQKS010000719         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_2096189, whole genome shotgun sequence.
ACCESSION   DASQKS010000719 DASQKS010000000
VERSION     DASQKS010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096189"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VE170_18550"
     CDS             <1..821
                     /locus_tag="VE170_18550"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYJ17503.1"
                     /translation="TTQGEVPDSAMQLATRLSSLQLIVAIKRRMSDQRRRLVIRGVIF
                     IGLMLLLFFFSRYIPRPFSAFAPPGAELDDFEGIYSSSDKLGKFLQQLGPYSPAVFVV
                     FQILQVIAAPFPGELTGVAGGFVYGETIGFILSTLGLSIGSWVAFELASILGRPFVER
                     FVKREVLEKFNFLTTKTGVAICFVLFLFPGFPKDYLCYVLGLSRMSVTTFLLVSVIGR
                     IPGTYLLTMQGATIRNQEYRTFAIIMAVTLLALVIGYIYRNQIFAYIKHRHDHD"
     gene            complement(868..1692)
                     /locus_tag="VE170_18555"
     CDS             complement(868..1692)
                     /locus_tag="VE170_18555"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYJ17504.1"
                     /translation="MNVFAIAAVLGIVEGLTEFLPVSSTGHLIIAGHLLGFVGDKAAS
                     FEVAIQLGAILSVVWLYWRRFVGLVPGTRQFTAMAASSLNGWPGLWRIALATLPALAA
                     GYLARHWIKEKLFNPEAVTLALAVGGIAILLVERLAARRRANDLDEVTVVQALGVGLF
                     QILALWPGTSRAAATIVGGMILGLERKAAAEFSFLIAVPVLCAASGYELLKMRDRLVF
                     DDAVTLVIGLVVSFAVALLAIKGFVSYLSHGKLAPFAWYRIVVAPIFYYLTRGSGL"
     gene            complement(1781..>2535)
                     /locus_tag="VE170_18560"
     CDS             complement(1781..>2535)
                     /locus_tag="VE170_18560"
                     /inference="COORDINATES: protein motif:HMM:NF037277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17505.1"
                     /translation="HYILPSFPPLAILLGTAVSRLLSDSQDRLRYGFGASWVVLSSAF
                     IYLAVGVIVPEIFPKIIRGRYDSIAILFWSSALVSTALVYFACKRDFWTAANVRSVYF
                     SQGFGLLILLLTMSEMMILIAPARSSQEIAVKALPYITPTTQLVSYDTYSEALPFYLK
                     TDKPLWVVTHSQKKRTFLGNFYEMTNQPDPITRWGKSLLTFDEFADRWKSSDTAFVIL
                     VKEKNLRAMERLVGVAAKRLAAHGEYLIVARP"
BASE COUNT          577 a          765 c          661 g          532 t
ORIGIN      
        1 gaaccacgca aggcgaagtg cccgatagcg ccatgcaact ggcaacgcgg ctctcgtctt
       61 tgcaattaat agttgctata aagcgccgga tgagcgatca gcggcgccgg ctcgtcatac
      121 gcggggtaat cttcatcggc ctcatgttgt tgctgttttt tttcagccgc tacattccgc
      181 ggccgtttag cgccttcgcg cctcccggcg ccgagctcga cgatttcgag ggcatctatt
      241 ccagcagcga taagctgggc aagtttctcc agcagctggg cccctattcg ccggcggtct
      301 tcgtggtgtt tcaaatcctc caagtgatcg ccgcgccctt ccccggcgag cttaccggcg
      361 tcgccggcgg ttttgtctac ggcgagacca tcggatttat tctctccacc ctcggcttat
      421 ccatcggctc ttgggtcgcc tttgagctcg cgagtatcct cgggcggccc tttgtcgagc
      481 gcttcgtcaa gcgcgaggtc ctggaaaaat tcaatttcct caccaccaag accggcgtgg
      541 cgatctgctt cgtgctgttt ctgtttcccg gcttccccaa agattacctc tgctacgtgc
      601 tcggcttgag ccgtatgagc gtcactacct tccttttggt atccgtcatc ggccgcatac
      661 ccggcaccta tcttctcacc atgcaaggcg caacgattcg caatcaggag tatcgcactt
      721 tcgcgatcat tatggctgtc accttgctcg ccctcgtaat cggttatatt tatcgcaatc
      781 aaatcttcgc atacatcaag caccgccatg atcatgacta gaaaccggct ggtcagggcg
      841 gtcgcacttc gcgcttaatc gtgatgctta cagccccgag ccgcgcgtga ggtagtaaaa
      901 tatcggcgca acgacaatgc ggtaccaggc aaatggcgca agtttgccgt ggcttagata
      961 gctgacaaag cctttgatcg ccagtaacgc cacggcgaat gaaaccacca agccgatcac
     1021 cagggtaaca gcatcgtcga ataccaatcg atcgcgcatt tttagcagct cgtagccgga
     1081 cgccgcgcac aataccggga cggcgatcag aaacgaaaat tccgccgccg ccttgcgctc
     1141 aagtccgagt atcataccgc cgacaatcgt tgccgccgct cgtgatgtgc cgggccagag
     1201 cgccaagatt tgaaataacc cgacgcccaa tgcctgcacc accgttactt cgtccaaatc
     1261 gttggcgcgg cggcgcgcgg cgagccgctc gacgagtaga atcgcaatac ccccaactgc
     1321 caatgccaag gtgacggctt ccggattgaa gagtttttcc ttgatccagt ggcgcgccag
     1381 gtagcccgcc gccaacgccg gcagcgtcgc cagagcgata cgccacagcc ccggccaacc
     1441 attcaaactc gatgcagcca tcgccgtgaa ttgcctcgta ccgggcacca aacccacaaa
     1501 acgtcgccag taaagccaaa ccacggagag gatcgcaccc aattgaatcg ccacttcgaa
     1561 gctggcggcc ttatcgccga cgaagccgag caaatgaccg gcgataatca ggtggccggt
     1621 ggaggacacc ggcaaaaact cggtgagacc ctcaacgatc cccaacaccg cggcgatggc
     1681 gaaaacattc atgcagttcc ttccgattga cagtcgtgct gcttacgccg atggcgcccg
     1741 gcgctgccca acaagtgcaa cgtcaggcac atcaggttgg ctatggtcgg gcaacgatta
     1801 aatattcgcc gtgcgccgca agtcttttgg cagccacgcc gacgagccgt tccatagcgc
     1861 gtagattctt ctcttttacc aggattacaa atgccgtgtc ggagctcttc caacgatcgg
     1921 cgaactcgtc aaaagttaac agagattttc cccagcgcgt gatcgggtcg ggctgatttg
     1981 tcatctcata aaaattaccc aagaacgtgc gtttcttttg gctgtgggtg accacccaca
     2041 gcggcttgtc tgttttgaga taaaatggca acgcttcgga ataagtatcg taggaaacaa
     2101 gctgtgtcgt tggggtgata tacggcagcg ccttcacggc gatttcctgg gaggatctgg
     2161 ccggcgcgat caagatcatc atctcgctca tcgtaagcaa taagatcaac aagccgaagc
     2221 cctgactgaa gtaaacagat cggacgttcg ccgctgtcca gaaatcccgc ttgcatgcga
     2281 aataaaccag cgccgttgaa accaacgcgc tgctccagaa caaaatggcg atgctatcgt
     2341 agcgcccgcg gataatcttg ggaaaaatct cgggcacgat cacgccgacg gccaagtaga
     2401 tgaatgctga acttaaaaca acccagctcg cgccgaagcc gtagcgcagc cggtcttgag
     2461 aatcgctcaa taggcgactc acggcggtgc cgagcaagat cgccagtggt ggaaagctcg
     2521 gcaaaatata atgcg
//
LOCUS       DASQKS010000720         5405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4597562, whole genome shotgun sequence.
ACCESSION   DASQKS010000720 DASQKS010000000
VERSION     DASQKS010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4597562"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="argH"
                     /locus_tag="VE170_18565"
     CDS             complement(<1..805)
                     /gene="argH"
                     /locus_tag="VE170_18565"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYJ17506.1"
                     /translation="MNKPKQKLWGGRFNANTADSVEAFTASIAIDARLYRHDIMGSVA
                     HAKMLGKQRIIPRRDAQKIVRGLAAIEREIENGKFEFSPADEDIHMNIERRLTERIGS
                     AGGKLHTARSRNDQVALDMRLYLRDEVAAILALLKTLQQELVKAAKKHLDVIMPGYTH
                     LQRAQPVLLSHHLLAYYDMFERDRERYAGCLERINVLPLGSGAIAGTTFPIDRAYVAK
                     LLGFPRISKNSIDAVSDRDFLLEFLAASSILFVHMSRFADELVLWSSQEF"
     gene            complement(895..2094)
                     /locus_tag="VE170_18570"
     CDS             complement(895..2094)
                     /locus_tag="VE170_18570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYJ17507.1"
                     /translation="MNVKKVVLAYSGGLDTSVILRWLKEEYGCEVIAFCADLGQGEDL
                     QAVKKKALQTGASKVVIGDLREEFVKNYVFPMLRANAVYEGSYLLGTSIARPLIAKGQ
                     IDVAVQEKADAVSHGATGKGNDQVRFELTYYALKPGIKVIAPWREWNLHARSTLIDYA
                     RKHGIPVPVTKAKPYSTDRNLFHISYEGGVLEDPWKEPPADMFVWCRPVEQAPNRAEY
                     IEIDYLRGDPLAVNGKKLSPANLLARLNDIGAKHGIGRADVVENRYVGMKSRGVYETP
                     GGTILHGAHRAIESITMDREVLHLRDSLMPRYAEMIYYGYWFAPEREVLQKAIDAAQA
                     NVTGRVRMKLYKGNVTVVGRKSDASLYDANVATFEADQVYRQADAEGFIRLNALRLRM
                     RRLAKAK"
     gene            complement(2091..3071)
                     /gene="argF"
                     /locus_tag="VE170_18575"
     CDS             complement(2091..3071)
                     /gene="argF"
                     /locus_tag="VE170_18575"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYJ17508.1"
                     /translation="MNKRDLLNFDGMSRREFDGLLQLADELKRKQRRGIAHAWLAGKQ
                     LAMVFEKPSLRTRATFNVGMIQLGGSAVYLGPAEVGLGTRETPADCARNLDRWFDLIT
                     VRTFGHQIIEELAEHAAVPVINALTDGYHPCQVLADCQTLIEHKGTLDGLKIAFVGDG
                     NNMVHSWLEAAAILPFAFALACPKGYEPNAAILQKARDQGAKITITQSVKEAADGADA
                     IYTDVWTSMGQEKEVQNRLRAFRAYQVNGQVVALAKPDALVMHCLPAHRGEEITHEVL
                     ESPQCVAFDQAENRLHAQKAVMVWLLKGFKGSRVQGSMKTTGSRSSKKSK"
     gene            complement(3092..4279)
                     /locus_tag="VE170_18580"
     CDS             complement(3092..4279)
                     /locus_tag="VE170_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYJ17509.1"
                     /translation="MKNKDIAAITDKFIAKTYARTPLALVKGRGLKVWDADGKEYLDF
                     LAGIAVNSFGHCHPAIVKAIKQQAQTLMHVSNLYHMQPQSELARELCRNSFADRVFFC
                     NSGAEANEAAIKLARRYGLEKLGGKYEIISTHNSFHGRTLATLTATGQEKVRAGYDPL
                     PAGFRQVPYNKLDAMEAAIDEKKTVAILIEPIQAEGGINVPHETFMLGLREICDQRRI
                     LLIFDEVQTGMGRTGKLFGYENFGVKPDIMTLAKALGGGLPLGAMLAREDVANSFGPG
                     SHASTFGGNPVSCAAGLAVMQLLKTGALKNCVQMGKYFVKGLEALKKRFSFIRDIRGK
                     GLLIGVELEIDGNRIAEACMLEGLLMNCTAAKVLRFVPPLTIKKSEIDRGLAILEKVL
                     ARQ"
     gene            complement(4276..5160)
                     /gene="argB"
                     /locus_tag="VE170_18585"
     CDS             complement(4276..5160)
                     /gene="argB"
                     /locus_tag="VE170_18585"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYJ17510.1"
                     /translation="MDDFIGKAEVLMEALPYFQRFYGKTFVVKYGGHAMENEELKASF
                     AQDIVLLKYVGINPVVVHGGGPQIDAALDKMGVKSHYVRGMRVTDQATLDIVEMVLVG
                     KVNKEIVNMINQHGGMAVGLSGKDGQLILAKKMNMTLASANGESPEIIDIGMVGEIIG
                     INPLIIDSLDANKFIPVIAPVGVGKNGETYNINADLVAGEVAEALHAEKLLLMTDVEG
                     VKDKKGELLSHLTEKQARKMIQDGVVGAGMIPKVECCINALKGGVEKTHIIDGRVKHA
                     VLLEIFTKEGVGTEVVRK"
BASE COUNT         1065 a         1614 c         1478 g         1248 t
ORIGIN      
        1 cgaactcttg actcgaccac aacaccaact cgtcggcgaa ccggctcata tgaacaaaca
       61 ggatcgaaga ggccgcgagg aactccagca gaaaatctcg atcgctcacc gcgtcgatgc
      121 tgttttttga aatccgcgga aaacccagga gcttggcgac atacgctcta tcgatgggga
      181 aagtcgtgcc ggcgatggcg ccggagccca gcggcagcac gttaatgcgc tccaaacagc
      241 cggcatagcg ctcgcggtcg cgttcaaaca tgtcgtagta agccagcaaa tgatgcgata
      301 acagcaccgg ctgcgcccgt tgcaaatggg tatagcctgg catgatgacg tcgaggtgct
      361 tcttcgccgc cttaaccagc tcctgttgca gagttttgag cagggcaaga atcgccgcca
      421 cctcgtcgcg caaatagagc cgcatatcca aggcgacctg gtcgttgcgg ctgcgcgccg
      481 tgtgcagctt gccgccggcg ctgccgatcc gctcggtcag ccggcgctct atgttcatgt
      541 gaatgtcttc atccgccggt gaaaactcga acttaccgtt ttcaatctcc cgctcgatcg
      601 ccgccaggcc gcggacaatt ttctgcgcat cgcgtcgggg aataatccgc tgcttgccga
      661 gcattttggc atgagcgaca ctgcccatga tgtcatggcg gtacaggcgg gcatcgatag
      721 cgatggacgc ggtaaaggcc tccaccgaat cggcggtgtt ggcgttaaat ctaccgcccc
      781 aaagtttttg tttcggcttg ttcacgataa aattttgcgc ccttgcgtca acggagattt
      841 gacaatgagc gacggcggca ttggcgaatt tccattctta gttttcagtt tgtctcactt
      901 cgccttggcc aaccgccgca tgcgcagccg caacgcattg agccggatga acccttcggc
      961 gtcggcctgg cggtaaactt gatcggcctc gaaggtcgcc acgttggcat cgtagagcga
     1021 ggcgtcggac ttgcgcccga ccaccgtgac gttgcctttg tataatttca tccgtacccg
     1081 gccggtgacg ttggcctgag cggcgtcaat ggctttttgc agcacttcgc gctccggcgc
     1141 gaaccagtag ccgtaataaa tcatctcggc atagcgcggc atgagcgaat cgcgcaagtg
     1201 cagcacctcg cgatccatgg taatcgactc gatggcacga tgggctccat ggagaatagt
     1261 cccgcctgga gtttcgtaaa caccgcgcga tttcatgccg acataacggt tctcgaccac
     1321 atcggcacgg ccgatgccat gcttggcgcc gatgtcgttc aagcgcgcca agagattcgc
     1381 cggcgagagt tttttgccgt tcactgccag cggatcgccg cgcagataat cgatctcgat
     1441 gtattcggcg cggttgggtg cctgctccac cggtcggcac cagacgaaca tatccgccgg
     1501 cggttccttc cagggatcct cgagaacgcc gccttcgtaa ctgatgtgaa acaagttgcg
     1561 atcggtgctg tagggtttgg ccttggtgac cggcaccggg atgccatgct tgcgcgcata
     1621 gtcgatcaag gtcgagcgcg cgtgtaaatt ccattcgcgc cagggagcga tcacttttat
     1681 gccgggtttg agcgcatagt aagtcagctc gaagcgcact tgatcgttgc ccttgcccgt
     1741 ggcgccatga gagacggcgt cggctttttc ttgcacggcg acgtcgatct gccctttggc
     1801 gatcaacggc cgggcaatgg atgtgccgag caaatacgat ccttcgtaaa cggcgttagc
     1861 tcgcagcatc gggaatacat aatttttgac aaactcttcg cgcagatcgc cgatcaccac
     1921 tttgctggcg cccgtctgca gcgctttttt cttgaccgcc tgaagatctt cgccctgccc
     1981 caggtcggcg cagaaggcta tgacttcgca gccatattcc tctttgagcc agcgtaaaat
     2041 gaccgacgta tccaaaccgc ccgagtaagc taatactact ttcttgacgt tcatttagat
     2101 ttcttcgaac tcctgctccc ggttgtcttc attgaacctt gaacccttga acctttgaac
     2161 ccttttagaa gccagaccat caccgccttt tgtgcatgca ggcggttctc cgcttgatcg
     2221 aaggcgacac actgcgggct ttccagcact tcgtgggtga tctcctcgcc gcggtgcgcc
     2281 ggcaggcaat gcatgaccaa agcatccggt ttcgccaagg cgacgacctg gccgttgacc
     2341 tggtaggcgc gaaaggcgcg caggcggttt tgcacctctt tttcctggcc catgctggtc
     2401 cagacatcgg tatagatcgc gtcggcgccg tccgccgcct ctttgaccga ttgcgtaatc
     2461 gtgatcttcg ccccttggtc gcgcgccttt tgcaaaatcg ccgcattggg ttcgtaacct
     2521 ttggggcagg ccagggcgaa cgcaaacggc aagatggcgg cggcttccaa ccaggaatga
     2581 accatgttgt tgccgtcgcc gacgaaagcg atcttcagac cgtcgagcgt gcccttgtgc
     2641 tcgattaacg tttggcaatc ggcaagcacc tggcagggat ggtagccatc ggtgagcgcg
     2701 ttgatcaccg gtaccgcggc atgttctgcc aactcttcga tgatctggtg gccaaacgtg
     2761 cgtaccgtga tcagatcgaa ccagcggtct aaattccgag cgcagtccgc cggcgtttcc
     2821 cgcgtcccca agccgacctc ggcgggaccc aagtaaaccg cgctgccgcc caactggatc
     2881 atgccgacgt taaaggtcgc gcgcgtgcgc agactgggct tttcaaatac cattgcgagc
     2941 tgtttgccgg ccaaccaagc atgcgcgatg ccgcgccgct gtttgcgctt caactcgtcg
     3001 gcaagttgca acaggccgtc gaactcccgg cggctcatgc cgtcgaaatt aagcaaatcg
     3061 cgtttgttca tcgaatttat gatgctgatg gctattggcg ggccagcact ttttccagga
     3121 tcgctaagcc gcggtcgatt tcgcttttct tgatcgtcag cggcggcacg aagcgcagca
     3181 ctttggccgc cgtgcaattc atcagcaagc cttcgagcat gcacgcttcg gcgatcctgt
     3241 tcccgtcgat ttccagttcc acgccgatga gcagcccctt gccgcgaatg tcgcggataa
     3301 acgaaaaccg ttttttcaaa gcttcgagcc ctttgacgaa gtatttaccc atctgcacgc
     3361 aattcttgag ggcgccggtt ttgagcagct gcatcaccgc caaaccggcg gcgcaagaaa
     3421 ccggattgcc gccgaacgtc gacgcgtgac tgcccggacc gaaactgttg gcgacatctt
     3481 cgcgcgccag catggcaccc aggggcaagc cgccgccgag cgccttggcc aaggtcatga
     3541 tatcgggttt gacgccaaag ttttcgtaac cgaacagctt gccggtgcgg cccatgccgg
     3601 tctggacttc gtcgaagatc agaagaatgc gccgttgatc gcagatctcg cgcagaccca
     3661 acataaaagt ctcatggggc acattgatgc cgccctcggc ctgaatcggc tcgatcagga
     3721 tcgccacggt ttttttctcg tcgatcgccg cttccatggc gtcgagcttg ttgtacggca
     3781 cctggcgaaa gccggcgggc aacggatcgt agccggcgcg aactttttcc tgccccgtgg
     3841 cggtcagggt cgccaaggtg cggccgtgga aagagttgtg ggtcgagatg atttcatact
     3901 tgccgccgag cttttccagg ccgtaacggc gcgcgagttt tatcgccgct tcgttagcct
     3961 cggctccgct attgcaaaaa aacacccggt cggcaaaaga gttgcggcag agttcgcgag
     4021 cgagctccga ttgtggctgc atgtggtaga gattggacac atgcatcaag gtttgtgcct
     4081 gctgtttgat tgccttgacg atcgccggat ggcaatggcc gaaactattg accgcaatgc
     4141 cggcgagaaa atcgagatac tccttgccgt cagcatccca aactttgagg ccccggccct
     4201 tgaccagcgc cagcggcgtg cgcgcgtagg tcttggcgat aaacttgtcg gtgattgcgg
     4261 cgatatcctt atttttcatt tgcgcaccac ctccgtcccc acgccctctt tggtgaaaat
     4321 ttccagcagc acggcatgct tcacccggcc gtcgatgatg tgagttttct ccacgccgcc
     4381 tttgagcgca ttgatacagc attcgacctt gggaatcata ccggcgccga ccacgccatc
     4441 ctgaatcatc ttgcgcgcct gcttctcggt gagatgactc aataactcgc cttttttatc
     4501 ctttacgcct tcgacgtcgg tcatcaataa tagtttttcc gcgtgcagcg cttcggcgac
     4561 ttcgccggcg accaggtcgg cgttgatgtt ataagtctcg ccgtttttgc cgacacccac
     4621 cggcgcgatc acgggaatga acttgttggc gtccaacgaa tcgataatca gtggattgat
     4681 gccgataatc tcaccgacca tgccgatgtc gatgatctcc ggcgactctc cgttggctga
     4741 ggctaaggtc atattcattt ttttcgccaa aatcagttgg ccatccttgc cgctcagccc
     4801 caccgccatg ccgccatgct ggttgatcat gttgacgatt tctttgttga ccttgccaac
     4861 cagaaccatc tctacgatat ccaaagtcgc ctgatcagtg acccgcatgc cgcgcacata
     4921 gtggcttttg acccccatct tgtcgagcgc cgcgtcgatt tgcggaccgc cgccgtggac
     4981 caccacggga ttgataccga cgtacttgag cagaacgata tcttgggcga agctcgcttt
     5041 gagttcctcg ttctccatgg cgtggccgcc gtatttgacg acaaaggtct taccgtaaaa
     5101 gcgctggaaa taaggcagcg cctccatcaa tacctcagcc ttgccgatga aatcgtccat
     5161 ctcgatcctt aatttatcgg ttatgcagat ttgccgtgaa gggccacgaa gatcatggtt
     5221 cgcctccgct caccgctgac ccgcgcaggc agttgtcggg cgaggtgcgc cgtcccctat
     5281 gatcgaacta ttcgcgtcct acgacagttc aaagtctacg ccgccgagtt tccgctgtgc
     5341 cactccaaag gcggattcat cccgtccgtg tatccaatgg aatttcttgc gacttgtgct
     5401 cttcg
//
LOCUS       DASQKS010000721         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4597758, whole genome shotgun sequence.
ACCESSION   DASQKS010000721 DASQKS010000000
VERSION     DASQKS010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4597758"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1043
                     /gene="murB"
                     /locus_tag="VE170_18590"
     CDS             102..1043
                     /gene="murB"
                     /locus_tag="VE170_18590"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYJ17511.1"
                     /translation="MARATDNAPLVHDLSSVSGARLKLDEPLARYTSMKIGGPADYFI
                     EADNGAALAQVLIALRRHQVPLYLLGNGSNVLVSDRGVRGAVIHLTGEFKNIKWHEGG
                     DHIEVEVGAAHAVTQLVRQAARKGYAGLEFAEGIPGSVGGALVMNAGAYGSEIEKVVE
                     RVDAINPDGQAMAFSRAQMTFSYRDSHLPAGTVVTGVVLRLRRDEAAQVNAKLRDLVS
                     RRKSSQPSGFPNSGSMFRNPPGDFAGRLIEACGLKGQRIGQAQIAERHANFIVNLGGA
                     KATEVRQLMELARSEVRRRFNINLVAEVKYLGEWLDK"
     gene            1054..1869
                     /locus_tag="VE170_18595"
     CDS             1054..1869
                     /locus_tag="VE170_18595"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HYJ17512.1"
                     /translation="MQLKVHRRDNHKRDKRLVKRRWLIAAIVPLLLIAGALIYRLSEP
                     LAIAARSTRDFFADNRYFAVRQIEVHSGDKVGGDEIVAIAGLRQGMNIWNVDAVAIEK
                     KIARHPWVRRVLVRRDFPRRVVIDVEERSPKAIVGVRKLYYIDADAVVFKEVASGESI
                     KYPMLTGVYAEGLIRGDPAARQRIRDAIHLTDLMAQHSHTLSEIHFEALDRLVVYPTK
                     FPVAWRLGWGDWEEKIARLERLLELWKGNEERLASLDMSFRDQVVARLREIKQ"
     gene            1910..3136
                     /gene="ftsA"
                     /locus_tag="VE170_18600"
     CDS             1910..3136
                     /gene="ftsA"
                     /locus_tag="VE170_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HYJ17513.1"
                     /translation="MAKKNTIVVGLDIGTSKVCAVVGEMTERGVDIIGVGSHASAGLR
                     KGVVINIESTVNSIKRAVQEAGLMAGCEIHTVFAAVSGGHIKSFNSHGIVAVKHKEVT
                     QRDLDRVIDAAKAVAIPLDREVLHVLPQDYIIDEQDGIKEPLGMSGVRLEAKVHVVTG
                     AVTSVQNIVKCCNRSGLHVAEIVLAPLAAAEAVLTDEERDLGVVLFDMGGGTTDITLF
                     HDGTVKHTAVLSIGGNHVTNDIAAGLRTPFGDAERIKQRYGYAKAVMAMDQERVEVPS
                     IAGKEAGTVSRQILCEIIEPRLDEIFELVQKEIAKSGFDGSLASGIVATGGGMLLSGA
                     VKMAERSFGLPVRLGVPMQIGGLVDVIDNPIHATAVGLVLHGMKGQERGVARQRDDRM
                     LAKVKHRMSDWLSEFF"
     gene            3179..4408
                     /gene="ftsZ"
                     /locus_tag="VE170_18605"
     CDS             3179..4408
                     /gene="ftsZ"
                     /locus_tag="VE170_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYJ17514.1"
                     /translation="MFEIVERQNSLTARIKVIGIGGGGGNAVNTMIGSKLTGVDFMVA
                     NTDAQSLEASQAPMRIQLGGLVTKGLGAGANPEIGRRAALEDQEQIREYMEGADMIFI
                     TAGMGGGTGTGGAPVLARIAREVGALTVGVVTKPFIFEGKKRQRQAEQGIEELKQSVD
                     TLIVIPNQRLLSIAAKTTTMIEAFHKADDVLLQAVRGISDLIITPGLINLDFADVRTV
                     MAEMGLALMGAASATGENRAIEAAQKAISSPLLEDISIQGARGVLINITGGPNLCLHE
                     VNEAASMIQEEAHDDANIIFGAVIDETMGDEIRITVIATGFGEEKHAHKTPVQVQPPA
                     ATSIPNIAGVPPKNKKVVHLGTIVDDLEGPTWHRKKSGSEDLESVTLNKNNFQSSANQ
                     EDDDKYDIPTFLRRQMD"
BASE COUNT         1023 a         1278 c         1507 g          923 t
ORIGIN      
        1 ggaacggagt aatggagtgg gagagtatcg gagtatcggc ggatgggggg cagcgcctca
       61 ctccattact ccttcacgcc aatcctactg agatgtttgc catggctaga gcaactgaca
      121 atgcgccgct ggttcacgac ctctcgtcgg tctccggcgc gcgccttaaa ctcgacgagc
      181 cgctggcgcg ctatacctcg atgaagatcg gcggtccggc ggattatttt atcgaagccg
      241 acaacggcgc ggcgctggcc caagttctca tcgctctgcg ccgccatcag gtgccgttat
      301 atttattggg caatggcagc aacgtcctgg tcagtgaccg cggcgtacgc ggcgcggtga
      361 ttcatctaac cggtgagttc aaaaacatta aatggcacga aggcggcgac catatcgaag
      421 tcgaagtcgg cgcggcccac gccgtcaccc agttggtgcg ccaagcggcg cgcaagggat
      481 atgccggtct ggaattcgcc gaggggattc ccggcagcgt cggcggcgcg ctggtgatga
      541 acgcgggagc ctatggttcc gagatcgaaa aggtggtcga gcgtgttgac gcgatcaatc
      601 ctgacggcca agcgatggct ttttcccggg cgcagatgac gtttagttat cgcgattcgc
      661 atctacccgc gggcaccgtg gtgaccggcg tcgtgctgcg tttgcgccgg gacgaagcgg
      721 cacaggtgaa tgccaaattg cgcgacctgg tgagcagacg aaaaagcagc cagccgtcag
      781 gatttcccaa ctctggctcg atgtttcgta atccgccggg ggatttcgcc ggccgattga
      841 tcgaagcctg cggtttgaag ggccagcgca tcggccaggc gcagatcgcc gagcgccatg
      901 ccaatttcat cgtcaatctc ggcggcgcca aagccaccga agtgcggcag ttgatggaac
      961 tggcccggag tgaggtgcgc cgacgtttta atatcaattt ggtcgccgaa gtgaagtatc
     1021 tcggcgaatg gctggacaaa tagtcgaatc cctatgcaat tgaaagttca ccggagagac
     1081 aatcacaagc gcgataagcg gcttgtcaag cgccgctggt tgatcgcggc cattgtgccg
     1141 ttgctgctga ttgccggcgc gctgatctat cgtctcagcg aaccgctggc cattgccgcg
     1201 cgctcgacgc gggatttttt cgccgacaac cgttatttcg ccgtgcgcca gatcgaggtg
     1261 cacagcggcg acaaggtcgg cggcgacgag atcgtcgcca ttgccggcct gcgccaggga
     1321 atgaatatct ggaacgtcga cgccgtcgcc atcgagaaaa aaatcgccag acacccttgg
     1381 gtgcgccggg tgttggtgcg ccgcgatttc ccgcgccggg ttgttattga cgtcgaggaa
     1441 cgcagcccca aagcaatcgt cggcgtccgt aagctgtact atatcgatgc cgatgccgtg
     1501 gtgttcaaag aagtcgcctc gggtgagagc atcaagtatc ccatgctcac cggtgtctat
     1561 gccgaagggc taatacgcgg cgatcctgcg gcgcgtcagc gcatccgcga cgccatacac
     1621 ttgaccgatt tgatggcgca gcattcccat acgctgtcgg agattcactt cgaagcgctg
     1681 gaccggctgg tggtgtaccc gacgaaattc ccggtggctt ggcgcctggg ttggggagac
     1741 tgggaggaga aaatcgcgcg cttggaacgc ttgctggaat tatggaaggg caacgaggag
     1801 cggttggcat ctttggatat gagcttccgc gatcaagtgg tggcgcgctt gcgagagata
     1861 aagcagtagc ggagccaaag gatcaactag caactgcaga tcgaaggcta tggcgaagaa
     1921 aaatactata gttgttggct tggatatcgg cacgtcaaaa gtttgcgccg tggtcggtga
     1981 gatgaccgaa cgcggcgtcg acatcatcgg tgtcggtagt cacgcgtcag caggcttgcg
     2041 caagggcgtt gtcatcaaca tcgaaagcac cgtcaactca atcaagcgcg cggtccaaga
     2101 ggcgggattg atggccggct gcgaaattca cacggtgttc gccgctgtct ccggcggcca
     2161 tatcaaatcg ttcaacagcc acggcatcgt cgcggtgaag cataaggagg tgacgcagcg
     2221 cgatctcgac cgggtgatcg acgccgccaa ggcggtggct atcccgctcg accgggaagt
     2281 gctgcatgtc ctgccccagg actacatcat tgacgagcag gacggcataa aagaacccct
     2341 gggcatgtcc ggggtccgct tggaagccaa agtccatgtg gtgaccggcg cggtcacctc
     2401 ggtgcaaaac atcgtcaagt gctgcaatcg cagcggcctc catgtcgccg agatcgtgct
     2461 cgcgccgttg gccgccgccg aagcggtgct tacggatgaa gagcgcgatt tgggagtggt
     2521 gctattcgat atgggcggtg gcaccaccga tatcacgcta tttcatgacg gcacggtgaa
     2581 acatacggcg gtcctcagca tcggcgggaa tcatgtcacc aatgatatcg ccgccggttt
     2641 gcgcacgccg tttggcgatg ccgagcgcat caagcaacgt tatggctacg ccaaagcagt
     2701 gatggcgatg gatcaggaac gggtcgaggt gccgagcata gccggcaagg aggcgggaac
     2761 ggtatcgcgg caaattcttt gtgaaatcat cgagccgcgc ctagacgaga tcttcgaatt
     2821 ggttcagaaa gaaatcgcca aatccggttt cgacggctcc ttggcgtcgg gaatcgtcgc
     2881 taccggcggt ggcatgctac tgtccggcgc cgtcaagatg gccgaacgct ccttcgggct
     2941 gccggtgcgc ttgggtgtgc cgatgcagat cggcgggttg gtcgacgtca tcgataatcc
     3001 gatacacgcc accgcggtcg ggctggtgct ccatggcatg aagggccagg agcgcggcgt
     3061 ggcgcggcag cgcgacgaca ggatgcttgc caaggtgaag caccgcatgt cggattggct
     3121 cagcgagttt ttttaaacgg ggataacgat tttcaagttt agtggaggag gagagaatat
     3181 gtttgaaata gtcgaacggc aaaatagcct gaccgcgcgc atcaaggtca ttggtatcgg
     3241 tggcggcggc ggcaacgcgg tcaataccat gatcggttcc aaacttaccg gcgtcgattt
     3301 catggtggcg aacaccgacg cgcagtcctt ggaagcgagc caagcgccga tgcggatcca
     3361 actgggcggc ttggtgacca aggggttagg tgcgggcgcc aatcccgaga tcggccggcg
     3421 cgctgcgctg gaggatcaag aacagatcag ggagtacatg gaaggcgccg acatgatttt
     3481 tattaccgcc ggcatgggcg gcggcaccgg cactggcggc gcgccggtgt tggcgcggat
     3541 cgcgcgtgaa gtcggcgcgc tgaccgttgg tgtggtgacc aagcctttca tcttcgaggg
     3601 caaaaaacgc cagcgccagg cggaacaggg catcgaggag ttgaaacaaa gcgtcgatac
     3661 cttgatcgtg attccgaacc agcgcttgct cagcattgcg gccaagacca cgaccatgat
     3721 cgaagcgttt cacaaggccg acgatgtctt gctccaagcg gtgcgcggca tctcggattt
     3781 gatcatcact cctgggttga tcaatcttga cttcgccgat gttcgaacgg tgatggcgga
     3841 aatgggcttg gcgctaatgg gcgcggcgtc ggcgaccggt gagaaccggg ccatcgaagc
     3901 ggcgcaaaaa gcgatctcca gtccgttgct cgaagatatc tcgattcagg gagcacgagg
     3961 cgtgttgatc aatatcaccg gggggccgaa tctgtgcctg cacgaagtca acgaagcggc
     4021 atcgatgatt caagaagaag cgcatgatga tgccaatatt atttttggcg cggtgatcga
     4081 tgaaaccatg ggcgacgaaa ttcgcatcac cgtgatcgct accggtttcg gcgaagaaaa
     4141 acatgctcat aaaacgccgg tgcaagttca gccacccgcg gcgaccagta tccctaacat
     4201 cgccggcgtc ccgccaaaaa acaaaaaggt ggttcatctc ggtaccatcg tcgacgatct
     4261 cgaagggccg acctggcacc gcaagaaatc tggcagtgaa gatctcgaga gcgtgacgct
     4321 caacaagaat aattttcaat cgagcgccaa tcaggaagac gacgataagt acgatatccc
     4381 gacgttcctg agacgacaga tggattagca atccgataat ctaggccctg ctccgctggg
     4441 tcggccgtcc gcagtccccg ctgtggacgg ccggctattc gccaaaaaca gctgggcggc
     4501 ggcagtcgtt cgcccgcgct gttattggcc ggcggcggcg cggtgtcggt gggggaaagc
     4561 ctgagtctcg ctatcgaatt atttcagata gcgcacacgg gtgccatggg ttaccgacag
     4621 gatgatttct tgcgctccaa tgtccttggg gaagagcacg ccggaaatcc gcgcgctatt
     4681 gaagctcgcc ccttcgagcg atgcttgcga gagatcgatg ccgcgcaaat c
//
LOCUS       DASQKS010000722         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4598388, whole genome shotgun sequence.
ACCESSION   DASQKS010000722 DASQKS010000000
VERSION     DASQKS010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598388"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1993
                     /locus_tag="VE170_18610"
     CDS             281..1993
                     /locus_tag="VE170_18610"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYJ17515.1"
                     /translation="MAAPASDRSQYFFHIVLLLALCGVLYFPYLGSTPMFDKGEPREA
                     LAVQDIVQRGEWLFPLKRATAIPSKPPMFHWSAALTYQVSGVLNEATVRFPSALYATL
                     GVVFLYLLGAKLFGAQVGLLGGAILATTMIYQTQAVNARVDMTLCFYVTLSLILFYAI
                     YRDFLRHPLWPYGMYLLIALATLSKGPLGIVLPGLVIAVFLAVKRNWQMFFKFAFHPG
                     VLLALVVIAGWYGMAITRGGEGFVSRQLLSENLSRFVGGSGHSHPVYYYLSYLFSLAA
                     PWGLFYPLMFWDWVKARRSTDGDDLLFLQTWFGVMLLFFSLSSGKRAVYLLPIYPALA
                     LLLAVWCRGQMNFGMVRTWFLRVVAGVAGITAVILLIIALGDLWTHDPTWLYRPIERM
                     LKPKDRANFLVIVDRIADFGWKFDAIALAWAGLWSLLGYYLWRGRMIAAGGSLVALSI
                     VMAFLSRGLVEATIAETKSYRDFMVAVNRLVDPAEKLVLYGGFNGDAVAFYRGGTVEV
                     AERPVGNNLAQIAAGSGYIITTEKVWNRIQQAQPYLTPPLLRSRGTGPEGDAPLVLLH
                     AVLS"
     gene            complement(1996..2976)
                     /locus_tag="VE170_18615"
     CDS             complement(1996..2976)
                     /locus_tag="VE170_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ17516.1"
                     /translation="MTAIKSLVGFLFVIALVMDSQPVLSQTLDKIRLGYSGTGINNYV
                     LDMGKRTGIFKKNGIDIEVVYVNSGSLLSQALIAGTFDLSFSQGSEAMMAKIRGADVR
                     IAGVVANRFNHVYLAAPSITSIKQLKGKKVAVSRFGSGSHFQTNLALKEGGLDPEKDV
                     TILQIGNSGARITAILSGVVDGTIMAADFVPRAKREGFNVLADLADTKIEYPFLSLNF
                     LGSYLDRNQKQVKALIRSMSESIRALQSDPATAKTVIRSALRTDDAATIDYALARSVQ
                     VLDRRPFPTPGGIQTVLDELGKDAKTKAWKFEDFVDLRALRELEKEGFFK"
BASE COUNT          567 a          825 c          896 g          774 t
ORIGIN      
        1 catgctacta tgcgtgatat tagcctccta gtgacgcatg gtcttacacg ggcccggaaa
       61 atgaatccag ggatttcttc cactaacctt tgtgcgttcc ctcatttttc caaagaggac
      121 gaacgacacc aagagttcga ataaactaat ctccgaactc gtgctccttg gtctttcgcg
      181 ggggaaacga tcttatgacg gaatgatccg gtattttgcc tcttccgatt tcctcgtttc
      241 tctttaacaa gccggatgaa aaggttatct aactgaattg atggcagcgc ccgcgtcgga
      301 tcgatcgcaa tacttctttc atatagttct cctgctggcg ctttgcggcg tgctctactt
      361 tccctacctc ggcagcacgc cgatgttcga caaaggcgag ccgcgcgagg ctttagccgt
      421 gcaggatatt gtccagcgcg gcgagtggtt gttcccgctc aagcgcgcca ccgcgattcc
      481 gtccaagccg ccgatgtttc attggtcggc ggcgctaacc taccaggtaa gcggggtgct
      541 aaacgaagcg acggtgcgtt tcccgtcggc gctgtacgcg actctcgggg tggtttttct
      601 ctaccttctc ggcgctaaac ttttcggcgc ccaggtcggt ttgctgggcg gcgccatcct
      661 ggcgacgacg atgatctatc aaacccaggc ggtgaacgcg cgggtcgata tgactttgtg
      721 tttctacgtt accctgagct tgatcctgtt ctatgccatt tatcgcgatt ttttgcgcca
      781 tccgctgtgg ccctacggga tgtatttgtt gatagctctg gcgactcttt ccaagggacc
      841 gctagggatt gttctgccgg ggttggtgat cgcggtcttc ctggcggtca aacgcaattg
      901 gcaaatgttt ttcaaattcg cctttcatcc cggtgtgctg ctcgcactag ttgtgatcgc
      961 cggctggtat ggcatggcga ttacccgagg cggcgagggt tttgtcagcc gccagctgct
     1021 gtcggaaaat ctcagccgct ttgtcggcgg ctcgggccat agtcatccgg tttattacta
     1081 tttgtcttat ctcttcagcc tggcggcgcc gtggggattg ttctatccgt tgatgttttg
     1141 ggactgggtc aaggcgcgcc gcagtacgga cggggacgac ctgctgtttt tgcaaacttg
     1201 gttcggcgtc atgctgctgt ttttctcgtt gtcttccggc aagcgcgccg tttatctctt
     1261 acccatatat cccgctttgg cgctgctctt ggcggtctgg tgtcgtggcc agatgaattt
     1321 cggcatggtt cgcacctggt ttcttcgggt tgttgccggc gttgccggga tcaccgccgt
     1381 gatcctgctg attatcgcct tgggcgatct atggactcat gatccgacct ggttgtaccg
     1441 acccatcgag agaatgctca agcccaaaga ccgtgctaac ttcctggtga tcgtcgatcg
     1501 gatcgccgac tttggttgga agttcgacgc catcgccctc gcatgggccg gtctatggtc
     1561 attgttgggt tattacctgt ggcgcggccg gatgatcgcg gccggcggct cgctggtcgc
     1621 gctgtcgatt gtcatggcat ttttgagccg tggcttagtg gaggcgacaa tcgccgagac
     1681 caagagctat cgtgacttca tggtcgcagt gaaccgactg gtcgatccag cggaaaagtt
     1741 ggttttgtac ggcggcttta atggtgatgc ggtggcgttc tatcgcggtg gaaccgtgga
     1801 ggttgccgaa cggccggtgg gcaataactt ggcgcaaatc gctgccggca gcggctacat
     1861 catcactacc gaaaaagtgt ggaacagaat ccagcaagct cagccttact tgacgccgcc
     1921 gttattgcgg agccgcggca ccgggcctga aggcgacgcg ccgctggtgc tgctgcacgc
     1981 ggtcctatcc tagcgctatt tgaaaaatcc ttctttctcc aactcgcgca gcgcgcgcag
     2041 atcgacgaag tcttcgaact tccacgcttt ggttttggca tcttttccta attcgtcgag
     2101 cacagtttga atgccgcccg gagtcgggaa cggccggcga tccaatacct ggacgctgcg
     2161 cgcgagagcg tagtcgatcg tcgccgcatc atcggtgcgc agcgccgacc ggatcactgt
     2221 cttggccgtg gccggatcgc tttgaagagc gcggatcgat tcggacatgc tcctgatcaa
     2281 cgccttgacc tgtttctgat tgcgatcgag atagctgccg agaaagttga gcgacaaaaa
     2341 gggatactcg attttggtgt cggcgagatc ggcgaggacg ttgaaacctt cccgtttggc
     2401 gcgcggcacg aagtccgccg ccatgatcgt gccgtcgacg acgccgctca ggatcgccgt
     2461 gatgcgcgcg ccggagttgc cgatttgtaa aatagtgacg tccttttccg gatcaaggcc
     2521 gccttccttg agcgctaagt tggtctggaa gtgggagccg gagccgaagc ggctgacggc
     2581 gaccttcttg cctttgagtt gcttgatcga ggtgatggac ggcgcggcga gatagacatg
     2641 gttaaagcgg ttggcgacaa cgccggcaat gcgcacgtcg gcgccgcgta tcttcgccat
     2701 catcgcctcc gagccctgtg aaaaggatag atcgaaggtg ccggcgatga gcgcttggct
     2761 gagcagcgag ccgctgttga cgtaaacgac ttcgatatcg atgccgttct ttttgaaaat
     2821 gcctgtgcgt ttgcccatat ccagcacata gttattgatg cccgtgccgc tataaccgag
     2881 gcgaatcttg tcgagggttt gactcaggac cggctgagag tccatcacca aggctatgac
     2941 aaataaaaaa ccgacgagcg atttgatggc tgtcattgaa tcggtctcct catggcgaaa
     3001 tgagcgcatc gtaatgccgg cggacggcaa gcacaagagt aaaatctgag catcatccat
     3061 ca
//
LOCUS       DASQKS010000723         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4609560, whole genome shotgun sequence.
ACCESSION   DASQKS010000723 DASQKS010000000
VERSION     DASQKS010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4609560"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VE170_18620"
     CDS             complement(<1..386)
                     /locus_tag="VE170_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17517.1"
                     /translation="MTWNNLRTNRANSPPSNSGFSARRLSASLLLSFAISLPLGCDPQ
                     SNLENGSYGKYLASPKLISSDKPAKVDRRQKIANNTVLEHNAEHRGRIRILFAGRGFE
                     PQSFDPVADGQTLIAPGIYHKAKAQGS"
     gene            complement(508..792)
                     /locus_tag="VE170_18625"
     CDS             complement(508..792)
                     /locus_tag="VE170_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17518.1"
                     /translation="MIEAQGIATLMIGTAHDIMSQVTPPRALFVDHPVGRTFGPPHDR
                     VRNQAVLARALEELPKFTRCGEIRDPGLQWLSDGSRTWEDELRAEMSLDH"
     gene            complement(829..1299)
                     /locus_tag="VE170_18630"
     CDS             complement(829..1299)
                     /locus_tag="VE170_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ17519.1"
                     /translation="MPFQYLPVMAQNSAPKPPRVLVSPTKLAWCQITKPLSESRVALL
                     SSAALRLAHQEPFIPREDFNYRRVPSNPSAGEIIIDHHSGIGRVPKQDPEIVFPRTAL
                     ANLHNRGVVGSLAPLHFSFMGGLRDHGKIENELAPAIANDLKQAQVDVALLVPY"
     gene            1606..2298
                     /locus_tag="VE170_18635"
     CDS             1606..2298
                     /locus_tag="VE170_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYJ17520.1"
                     /translation="MIDVGANYRAIVERIAVAAAKAGRDARDVRLLAASKSQPVNSIR
                     AALTAGVALVGENYVQEAEEKKFQLADAKVEWHMIGHLQRNKAKLAVELFDVVESLDN
                     FALAQELDKAAAKRSKIVRAFIEVNLAGEESKTGIAKGEVAGLLEKISGLAHMSVRGL
                     MTVPPFRENPEEVRPCFRELRELRDSLDSLHLPNVHLSELSMGMTHDFIVAIEEGATI
                     VRVGTALFGPRG"
     gene            2305..2604
                     /locus_tag="VE170_18640"
     CDS             2305..2604
                     /locus_tag="VE170_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HYJ17521.1"
                     /translation="MFLVANLLVAIAQILDYILWAYAWILLARVVISYINADPNNPLI
                     RFLYGATEPILSRVRAKLPLSSGGFDFSPVIVWLAVMFLQRFLVRSLYDLAQAIS"
     gene            2623..3576
                     /locus_tag="VE170_18645"
     CDS             2623..3576
                     /locus_tag="VE170_18645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYJ17522.1"
                     /translation="MKSLFAADIRDNQAVDAMFLVAAKTHGVTKTGNSYLTLRLIDRT
                     GDIEARVWERAEDLSRGFDKNDFVRVRGQATLYQGKMQLRVQDVMRVDESKIAPEDFL
                     PKSAFDPQSMLDELQTILRGIKNPYLLALAEACFADEELMRLLRQAPGAKTIHHPYLS
                     GLLEHTLSLMKLIQKVVENYQDVDVDLLLMGGFLHDIGKVYEFTFERTVDYTDAGQLL
                     GHLVMEVEMVTKKIEAISEFPTELGLLLKHLLVSHHGAYEFGSPKLPQTVEAVILHSL
                     DDLDGKIQAIQNLPDKEPGSKWTAFHRAYGRSFYRVKPEQH"
     gene            3668..5086
                     /locus_tag="VE170_18650"
     CDS             3668..5086
                     /locus_tag="VE170_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ17523.1"
                     /translation="MKNFVAGAWIDKAKKIEVTNPFDNSVIDAVPRADAGDLDMALAF
                     AERGAKVMAKLSSYERWKILRKAADMMAARNEELGQIISQEEGKIIAEGRGEAMRAVE
                     TIMGSAEEAKRLHGETVPLDADPTGGKKLGFTLRVPCGVVAAIAPFNFPLNLVCHKVG
                     PALAAGNTVIIKPASDTPLSAIKLTEILLEAGLPPEGIQCLTGSGGEIGDALVADRRV
                     RKVTFTGSREIGERICRTAGIKKVTMELGSNSPVIVMPDADMEKVATVLAMTGYGNAG
                     QTCISTQRVLAAKKVYGDLLSALKPKVEALTTGNQLDEKIKVGPMVKESEAVRVDAWI
                     NEAVSGGAKLVAGGGRHGAIYLPAVVADVHPDMRISRDELFGPAVALTPFETIDEAIA
                     MANDSIYGLAAGIFTDNVEWAMKFAREAEAGNLHVNWGSQWRVDLMPYGGLKDSGFGK
                     EGPKYAVNEMTELKMIVFHLNS"
BASE COUNT         1165 a         1266 c         1549 g         1158 t
ORIGIN      
        1 gatccttggg ccttagcctt gtgataaata cccggagcaa taagggtttg gccgtctgcg
       61 acagggtcga aagattgcgg ctcgaagcct ctaccggcaa ataggattct aatcctaccg
      121 cgatgctcag cattatgctc caacacggtg ttattcgcta ttttttgtcg gcgatcaacc
      181 ttagcaggtt tgtctgagct gattaacttt ggactcgcga ggtatttacc gtaagacccg
      241 ttttcaagat tcgattgagg atcgcacccc agcggcaggg agatagcaaa agagagcagt
      301 aaactcgcac tgagccgccg tgcagaaaat ccagagttgc ttggcggaga gtttgctcta
      361 tttgtgcgca aattgttcca ggtcacgaca ttaagtaact agtatatcac aatgtttcta
      421 tttcgtgtta ggggtctggg tgtgatggtt gctccagtta cgccgattcg gttgcgcagt
      481 cactagcggc ataagcagcc aatccgctca gtgatcgaga ctcatctcag cgcgcaactc
      541 atcctcccaa gtgcggctgc catccgacaa ccattgtaag cctggatcgc ggatttcgcc
      601 gcaacgtgtg aacttgggca gttcttcgag cgcgcgggcg agcaccgcct ggttacgaac
      661 gcgatcatgg ggcgggccga aggtgcggcc caccggatga tcgacaaaca gcgcgcgcgg
      721 cggcgtgact tggctcatga tgtcgtgcgc cgtgccgatc atgagagtcg caatcccctg
      781 ggcttcgatc acatgagcga tgagaccgac ggtctcgtgg cagaagggtc aatagggcac
      841 gagcaaagcg acatcgacct gagcttgctt caagtcgttg gcgatcgccg gcgccaactc
      901 attttcaatc ttgccgtgat cgcgcaaacc gcccatgaag gaaaagtgca acggcgctaa
      961 gctgccgacg actccgcggt tgtgcagatt cgccaacgcc gtgcgtggaa aaacaatctc
     1021 agggtcctgc ttcggcaccc gtccgatgcc ggaatgatga tcaatgataa tttctccggc
     1081 gctcggattt gacggcacgc gccgataatt aaagtcttca cgcggaatga acggctcctg
     1141 atgcgccaaa cgcaacgccg cgctgctcag tagcgcaacc cgcgattcac tcaatggttt
     1201 cgtaatctga caccaggcta gttttgtcgg cgacaccagc acacgcggcg gtttcggcgc
     1261 ggagttttgc gccatgacgg ggagatattg gaaaggcata aaatgattat cctccgttgc
     1321 cgcagttcta ctgcaggaac taatagggtg caacgcaagc gtcgcgtaaa gtagctttgt
     1381 tacaggactt cgcgaatgca aaacttcggg ggagatcccc atccagcccg gcgaattgtt
     1441 tggttccacg tattattggc gatcattctg tggccgcctg gaatgttagt ggccgacacg
     1501 catcttcata gccttcccaa aagaaattta ctccgctttg tttggcgatc atgaaacgcg
     1561 ccggtgtgtg aaatcgctcc tgcgttgtgg taagcagttg ccctcatgat cgatgtcggg
     1621 gcgaactatc gggcgattgt tgagcggatc gccgttgcgg cggcaaaggc ggggcgggat
     1681 gcgcgcgacg ttcggctgct ggcggcttcg aagtcgcagc cagtaaattc gatccgcgct
     1741 gctctgacgg caggggtggc gctggtcggt gagaattacg tccaggaggc ggaggaaaag
     1801 aagttccaat tggcagacgc taaggtcgaa tggcatatga tcggccattt acaacgcaat
     1861 aaggccaagc tggcggtgga gttgttcgac gtcgtcgaat cgctggataa ctttgcgcta
     1921 gcgcaggagt tggataaagc ggcggcgaag cggagcaaaa tcgtgcgggc gtttatcgaa
     1981 gtgaatttgg cgggtgagga gagtaagacg gggattgcca aaggcgaagt tgccggcttg
     2041 ctagaaaaaa tctccggttt ggcccacatg agcgtccgag gtctaatgac cgtgccgccg
     2101 tttcgggaaa atcccgaaga ggttcggccg tgctttcgcg aactgcgcga gttgcgcgac
     2161 tcgctcgatt cactccattt gcctaacgtg catcttagcg agttgtccat gggtatgact
     2221 cacgacttta tcgtggccat cgaagagggc gcgactatag tgcgcgtcgg cacggcgtta
     2281 ttcgggccgc ggggatgacg acacatgttt ctggtcgcta atttgctggt tgcgatagcg
     2341 cagattctcg actacatcct gtgggcctat gcttggatat tattggcgcg tgtggtgatt
     2401 tcttacatca atgccgatcc gaataatcca ttgatccgtt tcctatatgg cgcaaccgaa
     2461 ccgatattgt cgcgggtgcg ggcgaaattg ccgctgtcct ccggtggctt tgatttttcg
     2521 ccggtcatag tttggctggc ggtaatgttc ttgcagcgtt ttttggttcg ctctttgtac
     2581 gatttggcgc aagcgatcag ttaagagtgg gaggagatcg agatgaagtc tctttttgcc
     2641 gccgatatac gagacaatca agctgtcgat gccatgtttt tggttgcggc gaaaactcac
     2701 ggcgtgacca agaccggcaa cagttattta acgttgaggc tgatcgatcg cactggcgac
     2761 atcgaagcgc gggtatggga gcgtgccgag gatctcagcc gcggcttcga taaaaatgac
     2821 tttgttcgcg tgcgcggcca ggcgacgctt tatcaaggca agatgcaact gcgggtgcaa
     2881 gatgtcatgc gcgtcgacga atcgaagatc gcgccggaag attttttgcc caagagtgct
     2941 ttcgatccgc agtccatgct cgacgagctg caaacgattc tccgcggtat aaaaaatccc
     3001 tatctgctcg ccctggcgga agcgtgcttc gccgatgagg agctaatgcg cttgttgcgc
     3061 caggcaccgg gagccaagac gattcatcat ccgtatttaa gcggcttgtt ggagcatacg
     3121 ctttccttga tgaaactgat tcagaaagtc gtcgagaatt atcaagacgt cgatgtcgac
     3181 ctgttgctga tgggcggctt tctccacgac atcggcaagg tttatgagtt tacgtttgaa
     3241 cggacggtgg attacaccga cgccgggcag ttattgggcc atctggtcat ggaagtggag
     3301 atggtgacca aaaagatcga agcgatttcg gaattcccga ctgagctggg gttgctgctc
     3361 aagcatttac tggtgagcca tcatggcgct tatgagttcg gctcgccaaa gctgccgcag
     3421 accgtcgaag cggtgatcct tcattccctc gatgacctag acggcaagat ccaagcgatc
     3481 caaaaccttc cggataaaga gccgggatcg aaatggaccg cgtttcaccg cgcctacggg
     3541 cggagttttt atcgagtcaa gcctgaacag cattgaagtt tgcatcatca tgggcaatgc
     3601 gagatttaac ttgaattttg tgaaatcaga aatatgaaat ccgagattca aacggaggtt
     3661 tttcatcgtg aagaattttg ttgcaggcgc atggatcgat aaagcgaaaa aaatagaagt
     3721 aaccaatccg ttcgataact cggtgatcga cgccgtgccg agggcggatg ccggagactt
     3781 ggacatggcc ttggcctttg ccgagcgtgg cgccaaggta atggcgaaac tatcgagcta
     3841 cgagcgctgg aagattcttc gcaaagcggc cgatatgatg gcggcgcgca acgaagagtt
     3901 gggtcagatc atttcccagg aagagggaaa gatcatcgcc gaaggacgcg gcgaggcgat
     3961 gcgcgccgtc gaaaccataa tgggctcggc ggaggaggcc aagcggttgc acggcgagac
     4021 cgtgccgctg gatgccgatc ctacgggcgg caagaaactc ggcttcacgc tgcgcgtgcc
     4081 ttgcggcgtc gtcgcggcca tcgcgccgtt caattttcca ttgaacctcg tctgccacaa
     4141 agttggcccc gcgctggcgg cggggaacac ggttattatc aaaccggcct cggatacacc
     4201 actgtcagcg atcaaattga ctgagatctt gcttgaagca ggcttgccgc ccgaagggat
     4261 tcaatgcctc acgggttcag gcggtgaaat tggcgacgct ctagtcgccg atcgtagagt
     4321 tcgtaaggtg acttttaccg gcagccgcga gatcggtgaa cggatttgcc ggacggcggg
     4381 catcaagaaa gtcacgatgg agctgggctc caacagcccg gtgatcgtca tgccggatgc
     4441 cgacatggaa aaagtcgcga cggttttggc gatgaccggt tacggcaacg ccggccaaac
     4501 ttgcatctcg acacaacggg tattggcggc aaaaaaagta tacggtgatc tcttgagcgc
     4561 gttgaagccg aaagttgaag cgttgaccac cggcaatcag cttgacgaga aaattaaagt
     4621 cggcccgatg gtgaaggaga gtgaagcggt gcgcgtcgat gcatggatca atgaagcggt
     4681 cagcggcggt gcgaagttgg tcgccggcgg tggccggcac ggcgcgattt atcttccggc
     4741 agtagtcgca gacgttcatc ctgacatgcg catttcgcgt gacgagttgt tcggcccagc
     4801 ggtggctttg acgccattcg agacgattga cgaagcgata gccatggcga acgatagtat
     4861 ctatggatta gccgccggga tttttaccga taacgtcgaa tgggcgatga agttcgcgcg
     4921 cgaagcggag gcgggcaacc tgcacgttaa ctggggctcg cagtggcgcg tcgatctgat
     4981 gccctacggc ggactgaaag actccggctt tggtaaggag gggcccaaat acgcggtcaa
     5041 cgagatgacc gaactgaaga tgatagtgtt tcatttgaat agttgagctg gctccagcca
     5101 ttggccggcg atagcaagca atagattagg aagaaatt
//
LOCUS       DASQKS010000724         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate
            SMAG_U11918 k141_4619815, whole genome shotgun sequence.
ACCESSION   DASQKS010000724 DASQKS010000000
VERSION     DASQKS010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945641
            Sequence Read Archive: SRR6958552
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 13:31:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,736
            CDSs (total)                      :: 3,715
            Genes (coding)                    :: 3,690
            CDSs (with protein)               :: 3,690
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4619815"
                     /isolate="SMAG_U11918"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.2639 N 110.8632 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VE170_18655"
     CDS             complement(<1..478)
                     /locus_tag="VE170_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HYJ17524.1"
                     /translation="MRLVAARFSGEDIDCAKMARPERLSIGTANAGGCQLVNWVDLML
                     LAVLGIFGLRGFFRGLFREILSLAGLIVGFLVAARYGDVAARYAAQHWQFAPILLKGA
                     GFVAIFFVIYFGFSLAGWLLHRSAKMLFLTTVNRAGGIVIGVGKGAAVAALAIFLLL"
     gene            complement(530..976)
                     /locus_tag="VE170_18660"
     CDS             complement(530..976)
                     /locus_tag="VE170_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HYJ17525.1"
                     /translation="MDLKAEIQQAVKAAMKGGDTVTLSTLRLLLAALQNEEIRLRRAL
                     NAEEIHKIIGTLCKQRGEAIELYRKGGREELALKEEAELAILKEFLPQQLSDEEIKSA
                     IQASIDEAGASGPADLGKVMKLAMPKVAGRSDGKRVSDLAKALLGG"
     gene            complement(1003..1197)
                     /gene="rpsU"
                     /locus_tag="VE170_18665"
     CDS             complement(1003..1197)
                     /gene="rpsU"
                     /locus_tag="VE170_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HYJ17526.1"
                     /translation="MTGVRVRDNESIESAIRRFKKLCEKAGILAELRKREHYEKPSVK
                     RKKKAIAAKKRALRRVRHAF"
     gene            complement(1387..2118)
                     /locus_tag="VE170_18670"
     CDS             complement(1387..2118)
                     /locus_tag="VE170_18670"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HYJ17527.1"
                     /translation="MASFKKIAHRGASGHYPENSRLAFEKAIAAGADMIELDCQLSAD
                     GHVVVFHDERLARTTGARGKVYDKTLAQLRTLDIGKWRKPAFRGEKILTLEEALDILA
                     GNVGLCLEIKVAVGAPSGIELKLLFILSHYDYLERTVISSFDYRSLARVRELAPEAAL
                     GVIYGAGCREDPLAAAERLAATSIHVQKQLAQREFCHRAWQAGLDLYVWTVNDRREIG
                     MFVDLGVQGIFSDFPEKLALVGRTE"
     gene            complement(2353..3102)
                     /locus_tag="VE170_18675"
     CDS             complement(2353..3102)
                     /locus_tag="VE170_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HYJ17528.1"
                     /translation="MGKRQRLDLLLVERQLVASREEGRRRILAGEILVNRQPSTKAGN
                     LIDSAAPITVKASRPYVSRGGVKLEKALREFAIEVAGKYAIDIGASTGGFTDCLLVHG
                     AAKVIAVDVGYGQLDWKLRNDPRVVVFEKTNIRYLEPASLPFVPELATIDASFISLKL
                     ILPKVRSLIATQATVVALIKPQFEVGKGKVGKGGVVRSAQEHSRVIDEISRMALDCGY
                     QVLAVTESPLLGPKGNKEFLLHLAMNSFAAG"
     gene            complement(3081..>4638)
                     /gene="dxs"
                     /locus_tag="VE170_18680"
     CDS             complement(3081..>4638)
                     /gene="dxs"
                     /locus_tag="VE170_18680"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HYJ17529.1"
                     /translation="ISAALGMVEANALAGGKRKVVAVISDGGLSAGLTYEGLNSAGHL
                     DRDLIVILNDNEHFIDPRVGAVSSFLSRQFTTDLGIRLQKNLSTLLKSLPSGEHIKQA
                     ARKIRDSFLSLVTPGFLFESLGFQYVGPIDGHDIAAMIATLENIKKVEGPTLVHVLTK
                     KGKGFALAEQDPIKFHGVTPFHVLTGKAKKDKSPVPTYTEIFGRALVRLAKENPKIVG
                     ITAAMGSGTGIDKLQRELPERTYDVGIAEQHAVTFAAGMATEGYVPVVAIYSTFLQRG
                     YDEILHDVCLQNLHVVLALDRGGLVGADGPTHHGVFDFAYLRSIPNMVIMAPKDENEL
                     QHMLKTAIEHSGPISLRYPRGEGWDVGLDEQLHTLEIGTAELLRDGTDLIIAAIGNTA
                     IPALKAAQDLAPLGINAAVVNARFVKPLDGSLFRNLLTKMPRLITVEDHAISGGFGSA
                     LVEFLADEGITGVDIKRLGVPDRFIPHGTQDELKKICGFDKDAIAQAALQMMRRGKKK
                     SREGWERGSA"
BASE COUNT         1062 a         1400 c         1178 g          998 t
ORIGIN      
        1 aaagtaacaa gaaaatcgcc agcgccgcca ccgctgcgcc cttaccgaca ccgatgacga
       61 tgccgccggc gcgattgacg gttgttaaaa ataacatttt tgccgaacgg tgcagcagcc
      121 aaccggccaa gctaaaaccg aaataaatca caaagaagat cgcgacaaac cctgcaccct
      181 tgagcaatat cggggcaaac tgccaatgct gcgcggcata ccgtgccgcc acgtcgccgt
      241 aacgcgcggc aacgaggaaa ccgacaatga gtccggcaag ggataatatt tcacggaaga
      301 gacctctgaa gaatcctcgc aggccaaata ttccaagaac ggcaagcagc atcaaatcga
      361 cccaattcac caactggcac ccacccgcgt tggcggtacc gatcgacaat cgctccggcc
      421 gcgccatctt cgcacagtcg atatcttctc cagaaaatcg ggcggcaacc agtcgcatcg
      481 ggtgggtcgc tcaagcactc gatggccgga gcgacccaat cccgatacac taaccgccta
      541 acaatgcctt ggcgagatcg ctaacccgct tgccgtcgct gcgtcccgcc actttgggca
      601 tcgctaactt cataactttt ccgaggtcgg caggcccgct ggcgccagcc tcgtcgatac
      661 tcgcttggat tgccgatttg atctcttcgt cgctaagttg ctgcgggaga aattccttga
      721 ggatcgcaag ctcagcttcc tctttgagcg ccagttcctc gcggccaccc ttacggtaca
      781 actcaatcgc ttcgccacgt tgcttgcaca gtgtaccgat aatcttgtga atctcttcgg
      841 cgttgagcgc ccggcgcaga cggatttctt cattctgcag cgccgccaac aacaatcgca
      901 acgttgacag cgtcaccgtg tcgccgcctt tcatggcggc tttcacggct tgttgaatct
      961 ccgcttttaa gtccatcact gcgttggctc ttgatgtcac tcttaaaacg catgacgcac
     1021 gcgccgcagg gcacgttttt tcgcggcaat cgcttttttc ttacgtttaa cgctaggctt
     1081 ttcgtagtgt tctcgtttac gcaattcggc gagaatgccg gctttttcgc atagtttttt
     1141 gaagcggcgg atggcgcttt caatcgactc gttatctcga acacgaactc cagtcataga
     1201 aatgactcac ccccgatcca ggcagaccgc acataaaccg gaattgattt taaccgataa
     1261 atccttcaag tcaagcaaat cacttttaaa tctaactgtt tgcattaatt gatgagaggc
     1321 gaaacgaaat cgaacccggg tttgcagagg gcttgcaagc ggacaaacag agggcaattt
     1381 tgcttatcac tcagtgcggc caaccagcgc tagcttctcg ggaaaatcgg aaaaaatccc
     1441 ctgcacaccc aaatcgacga acatgccaat ttcacgccga tcattgaccg tccagacgta
     1501 gagatctaag cccgcctgcc aagcgcggtg gcaaaattcc cgctgcgcca gctgcttttg
     1561 cacgtggatc gacgtggcgg cgagccgttc agcggccgcg agagggtctt cgcggcaacc
     1621 ggctccgtat atgaccccta acgctgcctc gggagccaac tcgcgtaccc tggccaggct
     1681 gcggtaatcg aaagatgaaa tcaccgtccg ctccaaataa tcatagtggc tcaggatgaa
     1741 cagcagcttc aactcgatgc cggacggagc gccgaccgcg actttgattt ccagacacag
     1801 accgacgttg ccggcaagaa tgtccagcgc ctcttccagg gtcaagatct tctccccgcg
     1861 aaaggccggc ttacgccatt tgccgatgtc tagggtgcgc agctgcgcca gcgtcttatc
     1921 gtatactttc ccacgggcgc cggtagtacg cgcgagccgc tcatcgtgga acaccaccac
     1981 atgtccatcg gcgctcagtt ggcagtcgag ctcgatcata tcggcgcccg cggcaatcgc
     2041 tttttcgaac gccaaccggc tattttccgg gtaatgtccc gaggcgccgc ggtgggcaat
     2101 tttcttgaac gatgccatga gctattttta gattgccagg cacgacatgt taacgatgcg
     2161 cgaaaatttt cccgacaata gctctaaatt gccgtgccct gagagcatac ccatcaacaa
     2221 gaagttcacg aaaagttagg atgacacaaa ggcaaaaaat aatcgccccg acaatgcatt
     2281 cgtcatcttg gtggcgcgaa cctcgcttcc ctggtaatgg cggcgccgca ctagcgccac
     2341 gcgaaagcaa atttagcccg ccgcaaacga attcatagca agatggagca agaattcttt
     2401 atttcccttg ggccctaaga gcggcgattc ggtgacggcc aagacctgat agccgcagtc
     2461 gagcgccatg cgagaaatct cgtcgatgac ccggctatgc tcttgcgccg agcgcaccac
     2521 accgccttta ccgactttgc ccttgcccac ctcgaactgc ggcttgatca gcgccacgac
     2581 agttgcttgt gtcgcgatca gcgaacgcac tttcggcaga atcaacttga gagaaataaa
     2641 cgaggcgtcg atggtcgcga gttctggaac gaatggcaga ctggccggct ccagataacg
     2701 aatattggtt ttttcgaaaa ccacaacacg tggatcgttg cgcagtttcc aatcgagttg
     2761 gccataaccg acatccaccg cgatgacttt ggcagcgcca tggacgagca gacaatcggt
     2821 aaagccaccg gtggaagcgc cgatgtcgat ggcatatttg ccggcgacct cgatcgcaaa
     2881 ctcgcgcaac gccttctcta atttaacgcc gccgcggctg acataaggtc gcgaggcttt
     2941 aaccgtgatc ggcgccgcac tgtcgatcaa gttacccgct ttagtcgacg gttgccgatt
     3001 gactagaatt tcgccggcga gaatgcgccg ccgcccttcc tcccggctcg ccaccagttg
     3061 gcgctcgacc aagagcaggt ctaggcgctg cctctttccc atccctctct acttttcttt
     3121 ttacctcggc gcatcatctg caaggccgcc tgagcgatgg catctttgtc gaatccacag
     3181 attttcttga gttcatcctg tgtgccatgg gggatgaatc gatccggcac gccgagtcgc
     3241 ttgatatcca caccggtaat gccctcgtct gcgagaaatt ccaccagcgc gctgccaaag
     3301 ccgccgctga tcgcgtggtc ttcgactgtg ataagccgcg gcattttggt gagcagattg
     3361 cgaaagagac tgccatccag cggtttgaca aagcgcgcat tgaccaccgc cgcattgata
     3421 cccagagggg ccaagtcttg cgccgctttc agcgccggaa tagcggtgtt accgatagcg
     3481 gcgatgatca gatcggtgcc atcgcgcagc agctccgctg tgccgatctc caacgtatgc
     3541 agctgttcgt cgagccccac gtcccaaccc tcgccgcgcg gataacgcaa tgaaatcggc
     3601 cccgaatgct cgatagcggt cttgagcata tgctgcaact cgttttcgtc cttgggcgcc
     3661 atgatcacca tattggggat cgaacgcaga tatgcgaaat cgaacacccc atgatgcgtc
     3721 ggaccatcgg caccaaccaa gccaccgcgg tcgagggcca gcaccacgtg aagattctgc
     3781 aaacaaacgt catgaagaat ctcgtcgtag ccccgctgga gaaaagtcga atagatcgcc
     3841 accaccggca cgtacccctc ggtcgccata ccggcggcga aagtaaccgc atgctgttcg
     3901 gcaatgccga cgtcataggt gcgctcaggc aactcgcgct gcaacttatc gatgccggtg
     3961 ccactgccca tagccgcggt gatgccgacg atcttgggat tttccttcgc caagcgaacc
     4021 agtgctcgac caaatatctc ggtgtaagtc ggcaccggac ttttgtcttt ttttgccttg
     4081 ccggtgagca cgtgaaacgg cgtaacgccg tgaaatttga tcgggtcctg ttcggccagc
     4141 gcgaaccctt tgcctttttt ggtcaagaca tggaccaagg tcggcccttc gaccttcttg
     4201 atattttcca aagtcgcgat cattgccgcg atatcatgcc cgtcgatggg cccgacatac
     4261 tgaaatccca aactctcgaa aagaaatccg ggcgtgacca ggctcaaaaa agaatcgcga
     4321 atctttcgcg ccgcctgttt gatatgctcg ccgctgggca aactcttgag caaggtcgag
     4381 agattctttt gcaatcttat gccgaggtcg gtggtgaact gccggcttaa gaaagatgac
     4441 accgcaccga ctcgcggatc gataaagtgt tcgttgtcgt tgagaataac gatcaagtcc
     4501 cgatccagat ggcctgccga gttgagccct tcgtaggtca atcccgcact caagccgccg
     4561 tcgctgatca ccgcgaccac tttgcgtttc ccgccggcga gagcattggc ttccaccatg
     4621 cccagcgccg ccgaaatc
//
